LOCUS       DASVXS010000001         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1132115, whole genome shotgun sequence.
ACCESSION   DASVXS010000001 DASVXS010000000
VERSION     DASVXS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132115"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VF752_00005"
     CDS             complement(<1..628)
                     /locus_tag="VF752_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853958.1"
                     /translation="MRLEAAIVPDRGALAVTDDFFRSRELYDAEGVSHTLGVGDSMVA
                     PLVVREIEGEPLHDAVSPYGYPGAVVRAPVAIDEVDWSKSGLVSVFLRDRIGAEPSLH
                     GATLRSSVQIADPSKPLDLREQHRRHIRRNERDGYSVRVLEGAASEPEDREQFREAYR
                     ETMVRTEASARYFFDGAWFDTVLSSPLSWLVLVDPPGGGPIAAGAIAVE"
     gene            complement(636..1169)
                     /locus_tag="VF752_00010"
     CDS             complement(636..1169)
                     /locus_tag="VF752_00010"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8853959.1"
                     /translation="MSDANVLLRDFEPGDAAAVQRWFNNTDATHSLMEYRDSMDEEGA
                     RRWVDAAMRKDGRDRKWAVLVEGFDEPVGFTALYGVGGQTAPELGAMLGADGLRGKGV
                     GKHAESLTIQKGFDEFGAWRILGLIPAHNLPAQAVVKRLGFKHEGTMREHIRRPDGEL
                     IDCEVYGLLKSDWKGYP"
     gene            complement(1207..1770)
                     /locus_tag="VF752_00015"
     CDS             complement(1207..1770)
                     /locus_tag="VF752_00015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8853960.1"
                     /translation="MISRAIDIVVAGVALVFTSPIIAAAMIAVRLESPGSPIYKQRRV
                     GLDGEPFEIWKLRTMYVGSDRGPDRAIPKDDPSITRVGAFLRRTSLDELPNLINVLRG
                     EMAIVGPRPTIQVQVDQYTPRQRRRLEVKPGLTGWAQVNGRVGLPWPERIELDVWYVD
                     HRSLRLDLQILAKTVRLLVTGHGLYTS"
     gene            complement(1767..>2031)
                     /locus_tag="VF752_00020"
     CDS             complement(1767..>2031)
                     /locus_tag="VF752_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853961.1"
                     /translation="LRYASPFLHVIAFGTNIALLGSGWVYVLTFAAQLGLLLAAALAR
                     CIRARPFAIAYYYVTITASIAAGLFDWLRRGMPLTWEKPEGTR"
BASE COUNT          316 a          722 c          698 g          295 t
ORIGIN      
        1 tctcgaccgc gattgcgccg gcggcgatgg ggccaccgcc cggcgggtcg accagcacga
       61 gccacgagag cgggctcgag agcacggtgt cgaaccacgc gccgtcgaag aagtagcggg
      121 cgctcgcctc cgtgcgcacc atcgtctcgc ggtacgcctc gcgaaactgc tcgcggtcct
      181 ccggctcgga ggccgcgccc tcgagcactc gcaccgaata tccgtcccgc tcgttgcgac
      241 ggatgtggcg gcggtgttgc tcgcgcaggt cgagcggctt cgacggatcg gcgatctgca
      301 cgctcgagcg gagcgtcgcg ccgtgcagcg acggctcggc gccgatgcga tcccgcagga
      361 agacgctcac gaggccactc ttggaccagt ccacctcgtc gatcgcgacc ggcgcgcgca
      421 cgaccgcgcc cggatagccg tacggcgaca ccgcgtcgtg cagcggctcg ccctcgatct
      481 cgcggaccac gagcggcgcg accatcgaat cgcccacgcc cagggtgtgc gagacgccct
      541 cggcgtcgta gagctcgcgg gagcggaaga agtcgtccgt gaccgccaac gccccgcggt
      601 ccggcacgat cgcggcctct agacgcatgc gggagctacg ggtagccctt ccagtcggac
      661 ttcagcaggc cgtagacctc gcagtcgatc aactcgccgt ccggccgcct gatgtgctcg
      721 cgcatcgtgc cctcgtgctt gaagccgagc cgcttcacga ccgcctgtgc cggcaggttg
      781 tgcgcgggga tcagaccgag gatccgccag gcgccgaact cgtcgaagcc cttctggatc
      841 gtgagcgact cggcgtgctt gccgactccc ttgccgcgca ggccgtcagc gccgagcatc
      901 gcgccgagct ccggagccgt ctgcccgccc acgccgtaga gcgcggtgaa gccgaccggc
      961 tcgtcaaagc cctccaccag cacggcccac ttgcggtcgc gcccgtcctt gcgcatcgcg
     1021 gcgtccaccc agcggcgcgc accctcctcg tccatcgagt cgcggtactc catgaggctg
     1081 tgggtggcgt cggtgttgtt gaaccagcgc tgcacggccg cggcgtcgcc cggctcgaag
     1141 tcgcgcagta agacattggc gtcgctcatc gcggggccat cgtagtcagg acccgcgcca
     1201 tcgtggttag gacgtgtaga ggccgtgacc cgtcacgagc agacgaacgg tcttggcgag
     1261 gatctggagg tcgagtcgca gcgagcggtg gtccacgtac cagacgtcga gctcgatccg
     1321 ttccggccac ggcagtccga cgcggccgtt cacctgtgcc cagccggtca gccccggctt
     1381 cacctcgagc cgccgccgct ggcgcggcgt gtactggtcg acctgcacct ggatcgtcgg
     1441 ccgcgggccc acgatcgcca tctcgccgcg caggacgttg atcagattcg gcagctcatc
     1501 gagcgacgtg cggcggagga aagcgcccac gcgtgtgata ctggggtcgt ccttcgggat
     1561 cgcgcggtcg gggccgcgat cggagcccac gtacatcgtt cgcagcttcc agatctcgaa
     1621 cggctcgccg tccaatccca cgcgccgctg cttgtagatc ggcgagccgg gcgactcgag
     1681 ccgaacggcg atcatcgccg cggcgatgat cggcgacgtg aacacgagag cgacgcccgc
     1741 gacgacgatg tcgatcgcgc gcgagatcac cgcgtgccct ccggcttctc ccaggtgagc
     1801 ggcatgcccc gccgcagcca gtcgaacagg ccggcggcga tcgaggcggt gatcgtcacg
     1861 tagtagtagg cgatcgcgaa cgggcgggcg cgtatgcacc gcgcgagcgc cgcggccagg
     1921 agcagaccca actgcgcggc gaaggtgagt acgtagaccc agccgctgcc gagcagcgcg
     1981 atgttcgtcc cgaacgcgat cacgtgcagg aacggcgagg cgtagcggag g
//
LOCUS       DASVXS010000002         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_990849, whole genome shotgun sequence.
ACCESSION   DASVXS010000002 DASVXS010000000
VERSION     DASVXS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990849"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..107)
                     /locus_tag="VF752_00025"
     tRNA            complement(35..107)
                     /locus_tag="VF752_00025"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Gly,seq:gcc)
     gene            complement(222..294)
                     /locus_tag="VF752_00030"
     tRNA            complement(222..294)
                     /locus_tag="VF752_00030"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(259..261),aa:Ala,seq:ggc)
     gene            600..1490
                     /locus_tag="VF752_00035"
     CDS             600..1490
                     /locus_tag="VF752_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX8853962.1"
                     /translation="MTEVKRRRRRAAPAAGIAPNEPERDILDQYLYEVSTYPLLKAEE
                     EIQLAKKIRAGDQDALQELVKRNLRFVISVAKKYQNRGLPLIDLIGEGNVGLLTAARK
                     FDPDQGVKFISYAVWWIRQAILSALARQGRTVRVPLNRTADLSRIIKASEILRQKLNR
                     EPSPEELSQLTGLSVDVVQSLAALNTSDVRLDAPMDPEGDRALIERFVADEMPDTEEE
                     AMNRFLTDEIEQALNTLPPRDAKVLRLYFGLEGGREHTLEEIGSMLGVTRERVRQLRD
                     RALKRLREGDVGRALGSFAA"
BASE COUNT          286 a          571 c          532 g          241 t
ORIGIN      
        1 gacggctgct gccgagccgc agccggcgta ccgaagcggg aaacgggact cgaacccgcg
       61 accccaacct tggcaaggtt gtgctctacc aactgagcta ttcccgcgga cggcgctgct
      121 ggtgacggcg ggggagcccg gcgggttccg cgacgtgacc aggtggtgcc gatactaaca
      181 tgccggggta gtacgctgta ctaccgacgc ccttcgagga gtggagctga ggggaatcga
      241 acccctgacc tcttgaatgc cattcaagcg ctctcccaac tgagctacag ccccgagccg
      301 tcgttcccgc gggcggcgtg atccacgcaa ccctcaggaa acacacagta tagcgggggg
      361 tatactggaa gtcaaggaaa cacgggctgg cggaaatttg tccggccggt attttgcagc
      421 gcctttcgtg cgcaccgcgc ggagggtttt cggagggggc ttggcggccc caccggatcc
      481 gtttcagtag cgatgcgacc tgcttcacgg ccttcgaagc tcgcgaggcc acgcacgccc
      541 gcacgtcgca gctcaccata gatgcctacc tcaaccgccg cgttcgaggc acccagacca
      601 tgaccgaagt gaagcgtcgc cgtcgccgcg ccgcgcctgc cgctggaatc gcgcccaacg
      661 agccggaacg tgacatcctc gaccagtacc tgtacgaggt cagcacgtac ccgctcctca
      721 aggccgagga agagatccag ctggccaaga agatccgcgc gggcgatcag gacgcgctcc
      781 aggagctcgt gaagcgcaat ctccgcttcg tgatctccgt cgccaagaag taccagaacc
      841 gcgggctccc gctcatcgac ctgatcggcg aggggaacgt cgggctgctc accgccgcgc
      901 gaaagttcga tcccgaccag ggcgtgaagt tcatctccta cgccgtctgg tggatccgcc
      961 aggccatcct ctcggcgctc gcgcgccagg ggcgcaccgt gcgcgtgccg ctcaaccgca
     1021 ccgccgacct ctcgcgcatc atcaaggcgt cggagatcct ccgccagaag ctcaaccgcg
     1081 agccgagccc cgaggagctg tcgcagctca ccgggctctc cgtggacgtc gtgcagtcgc
     1141 tcgccgcgct caacaccagc gacgtgcgcc tcgacgcgcc gatggacccg gagggcgacc
     1201 gcgcgctcat cgagcgcttc gtcgccgacg agatgcccga caccgaggag gaggcgatga
     1261 accgcttcct caccgacgag atcgagcagg cgctcaacac gctgccgccg cgcgacgcca
     1321 aggtcctccg cctgtacttc gggctcgagg gcggccgcga gcacacgctc gaggagatcg
     1381 gctcgatgct cggcgtcacg cgcgagcgcg tgcgccagct gcgcgaccgc gcgctcaagc
     1441 ggctgcgcga gggcgacgtc ggccgcgcgc tcgggagctt cgcggcgtaa cgaagggagc
     1501 gggtgcggaa tcgggcatcg attccgcgct ccgagacgac gagcggacgc gagcctgcgg
     1561 gcctcgcgtc cgcttctcgt ttccggcccc gcgcatcgcg cgtccgcggc acaccgtgca
     1621 tatttgtgtg
//
LOCUS       DASVXS010000003         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_283869, whole genome shotgun sequence.
ACCESSION   DASVXS010000003 DASVXS010000000
VERSION     DASVXS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283869"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..485
                     /locus_tag="VF752_00040"
     CDS             291..485
                     /locus_tag="VF752_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853963.1"
                     /translation="MSEQRESSDGSTSGNRERSVAQDAERIADEWSGRMADWLVRAAA
                     RAKEEAEDIWAEAQAKRRGE"
     gene            942..>1748
                     /locus_tag="VF752_00045"
     CDS             942..>1748
                     /locus_tag="VF752_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853964.1"
                     /translation="MDEVRKVSPSPLTGRVLVEFAEAVEDIQPVLDAIADLEPPPDYS
                     EPEIPPHPLDPGPIIEGASKLAGAAIGLGLLMVRRATGANEAPVARAGPGEIAGCLAL
                     LETEAGIADRIEAAIGHRNKEIAFGLVGIVSMTSAGSALGLLYTAASAIPLVNEARAR
                     RGAWREYQRRLGDAQVGYAGAVVTLSPGERSPLRGVVRDGFGVAVALDGTPQRLAPGA
                     ELTSGSRVYGGPVTVELQEDGGFEPRPRPGPPPNTFYDRYLKTIPYAALAY"
BASE COUNT          275 a          594 c          635 g          244 t
ORIGIN      
        1 gcgatgtctc ccaaggtccg acggtgggcg cgcgagggag cggtctacgg cctggccggc
       61 gcgatgaagg cgggtgacgt gctggttgct acggcgcgtg gtgccgcaca cggggctaag
      121 gagggagtct cgggtaacca ccacgaaacg cgctcggcga gcgcttcgcg atcgagctcg
      181 tcgagctccc ggccgagcgg cgcctcgcgt tcgcagcgca agacagggtc gacgcgttca
      241 cgggcgtcca agcgatcgag cggtacgcgc gcaaagcaat cagctagcgg atgagcgagc
      301 agcgcgaatc cagcgacgga agtaccagcg gcaaccgcga gcgaagcgtc gctcaggacg
      361 ccgagcggat cgctgacgag tggtccggtc ggatggccga ctggttggtg cgggccgccg
      421 cacgggcgaa agaggaagcc gaggacatct gggcggaggc ccaggcaaag cggcgcgggg
      481 agtgaggaaa ggctccgctc cgcaatgacc gcgcggcgcg ccacggcctc gaggagctgg
      541 gtccgttgag cacggccgcg atgccgcggg tcgtgcactc gctgccgggc cgggtgcggg
      601 tgcacctgcc ggcggacccg ccgatcgatg agaccgcgct ggagcgtcgc ctctcctcag
      661 ttgccggcgt tcgaaaggtg cgtgccaatg cgcgcacgcg aaacgtgctc gtggagtacg
      721 accgcgcgcg cctggacgaa acgaacatcc tccgccggct gggctggacc gcccctcgag
      781 ctcccagccg ggagccgacc gcttcagaac atcattcgcg atcgcgctcg cggcgcgcac
      841 cgctcgtcac tcggagcagc gcgcgcaggg cacggatcgc cgtgcacggc ttggaccgag
      901 accccagcct cgggcgccag ctcgtcgatc gcctcgatcg catggacgag gtacggaagg
      961 tgagcccgag ccccctcaca gggcgtgtgc tcgtggagtt cgcggaagcg gtggaggaca
     1021 tacagccggt tctcgacgca atcgccgatc tcgagccgcc gcctgattac tccgagcccg
     1081 aaatcccgcc gcaccccctg gaccccggcc cgatcatcga gggcgcctcg aagctcgccg
     1141 gagctgcgat cggtctcgga cttctgatgg tccgccgcgc cactggcgcg aacgaggctc
     1201 cggtggcgcg cgccggacct ggcgagatcg ccggctgtct agcccttctc gagaccgagg
     1261 ccggtatcgc ggaccgcatc gaggcggcga tcgggcaccg gaacaaggag atcgcgttcg
     1321 gccttgtcgg catcgtctcg atgacgtcag ccggcagtgc cctcggactg ctgtacacgg
     1381 cagcttcggc gatccctctc gtgaacgaag cgcgtgcgcg gcgcggcgcg tggcgcgagt
     1441 atcaacggcg gcttggcgac gctcaggtcg gttatgccgg agcggtcgtc acgctctccc
     1501 cgggcgagcg ctcgcccctc cgaggcgtgg tgcgagacgg ctttggcgtc gcggtcgcgc
     1561 tcgatggcac gcctcagcgg ctggcgcccg gcgcagagct cacctcgggg tcgcgcgtct
     1621 acggaggacc ggtcaccgtc gagcttcagg aggacggcgg cttcgagccg cgcccgcgtc
     1681 cgggtccgcc cccgaatacg ttctacgacc gctacctcaa gacgattccg tacgccgcgc
     1741 tggcatac
//
LOCUS       DASVXS010000004        25542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_425589, whole genome shotgun sequence.
ACCESSION   DASVXS010000004 DASVXS010000000
VERSION     DASVXS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 25542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25542
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425589"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF752_00050"
     CDS             complement(<1..623)
                     /locus_tag="VF752_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8853965.1"
                     /translation="MSGLADAGLLVRRSVIRLLRQPANIVFSIAFPLLLLAVNAGGLD
                     AATKIPRFPADSYITFALAVPFVQGALFAANSAGTNFAMDIESGFLSRLSLTPLTRGA
                     LLGGQLAGVIVLGIAQAVIFLAVGEAAGAGFAAGAPGVLVLLALSVLITLGFGTLGAL
                     AALRTGSGEAVQGMFPLFFVLLFLSSMALPRNLIEIDWFRTVATYNPV"
     gene            complement(620..1636)
                     /locus_tag="VF752_00055"
     CDS             complement(620..1636)
                     /locus_tag="VF752_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8853966.1"
                     /translation="MSEANGIEVEGLVRVFKKGPRAVDGIDLEVAPGEIYGFLGPNGA
                     GKSTTVLMLTTLLPPTAGRARVAGHDIVEDGAAVRRSIGAALQDAALDPYLTGREHMR
                     LQAGLHGLSKADREARSNELLTRVGLTEAADRKVGGYSGGMKRRLDLGLALVHRPRIL
                     FLDEPTTGLDIQSRSALWDEVSRLSKEEGVTIFLTTQYLEEADVLADRVGIIDHGRIV
                     AEGTPAQLKAEIGRSSIEVVPADPGDRERLAGVLERFGDCSETSGDGVATRFGNGDEL
                     ADVVRALDAAEIRVARLSVHEPSLDDVFLEKTGRSLEGAGEEEDESVSEPGRVRGAPV
                     EASA"
     gene            1703..2137
                     /locus_tag="VF752_00060"
     CDS             1703..2137
                     /locus_tag="VF752_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8853967.1"
                     /translation="MGTATTKAQTEPASEAWELFMELFGTVRPRMLDIQAEYGLKPPQ
                     FFALDALDEPAPMSSIADLLRCDRSAVTWITDRLEERGYVERRSDGRDRRVKLLALTD
                     EGRRVREEIRARLAIPPEALTRLSPAEQRALRDLLRKALERE"
     gene            complement(2235..2633)
                     /locus_tag="VF752_00065"
     CDS             complement(2235..2633)
                     /locus_tag="VF752_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX8853968.1"
                     /translation="MLPIAADYPFLDVFWTVLIFFLWVAWIWVLVTVLIDIFRRDDIG
                     GWAKAGWVIFVVVLPWLGVLIYLIAQHDGMRERSVRQAQAQKQQFDEYVRDAASGRSS
                     ASEIAQAKQLLDNGTITQQEFEAIKAKALA"
     gene            complement(2792..2865)
                     /locus_tag="VF752_00070"
     tRNA            complement(2792..2865)
                     /locus_tag="VF752_00070"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2829..2831),aa:Asp,seq:gtc)
     gene            complement(2904..3503)
                     /locus_tag="VF752_00075"
     CDS             complement(2904..3503)
                     /locus_tag="VF752_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853969.1"
                     /translation="MTGSEGTSSRGRRGHALAALVAVCALTALALVPGTAAASPISCK
                     GHWGALSGDLKADVDSDNAIGIAFACSQNVRGFTVVTNRDIDVFGVQAMSFAPQDHAP
                     GVLTSCEGDIPGRGISCAADNAKDLIKAGYFVRSGIGFDVSPCVYDRSSAGALTASLI
                     VSSANGQTFGPFPLKAPKGCTKKYRRSEAAATKKAAKSR"
     gene            complement(3500..4981)
                     /locus_tag="VF752_00080"
     CDS             complement(3500..4981)
                     /locus_tag="VF752_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8853970.1"
                     /translation="MSRCGDCAAPLGPGQGWCLECGARQPGAREPVRAAAEPEPVAET
                     GSLPVRALPNPRVAAALVVGVLTLGIVIGGAAGGSGSLANTRQVVLLPPPATQPAAAP
                     QADSAPSGGNQDLSAGGSSTSRERHGVEAVSSFGGNAATAPAPQEAPAPAPDTTQPDD
                     TPAPAPSSGIKHVFTVVLAGASYDAAFGPASIAPYLANELAPQGVLLTQYSGLGVSSI
                     AGFDALVSGQKPTDATRAGCAVYGGDCLFPAKTKTIADQVGDAGLSWKVYAGDLERGP
                     DPAITACRHPAEGSPDDTTTERQGDGYALAHNPLVWFHSVLDGPDCGVRDVGLDSLEA
                     DLASADTAANWSLIVPNLCDGGWAVPCVDGRPGGLPQADAFLKQWVPKILDSPAYTDD
                     GLLAIVFDDAPAGARDKRTGALLLSPLLDAGTTSDKPFDHYALLGDVEELLGLKRLAA
                     AGDKRLPDLTALFHKGVTASSHRTHSLERRMPPAGTSQQAEDR"
     gene            complement(4978..5364)
                     /locus_tag="VF752_00085"
     CDS             complement(4978..5364)
                     /locus_tag="VF752_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX8853971.1"
                     /translation="MARLRDRLPGRRAARPAAPPAAEQPDTRRAELAARRERLARELA
                     ELQWDLGGLTYEMAIRDHFRLDVLVRRAARLQEVDAELGEIERLLKMEDAGATGSCPS
                     CGALHSRGAVYCWQCGTTLMEVTPAA"
     gene            complement(5370..6275)
                     /locus_tag="VF752_00090"
     CDS             complement(5370..6275)
                     /locus_tag="VF752_00090"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8853972.1"
                     /translation="MSTPETLELPRLGEPGESCGNCGAPLAPDQRYCLNCGLRRAEPR
                     VDYERALSAPPAGPPSQGRPAQPAAPPPGGLPVTWPIAAGGAAVLVVALAMGVLIGNT
                     TSGGKSSGSPQVITVGGAAGGAASGGATPAAAFKEDWPSGKSGWTVQVQALPKSGNGA
                     SQVAAAKQAATSKGAKDVGALDSDSHASLKGGFYVVYSGVFDTKKQAQKALAGLKKKF
                     PDARVVQVGDSAGASGGKNVKSLSGKESATANKKQLQQLNNLSPDQYQKQSKKLPSTL
                     KLPGKPPPVDKSKPVGGGSPTETIK"
     gene            complement(6283..7014)
                     /locus_tag="VF752_00095"
     CDS             complement(6283..7014)
                     /locus_tag="VF752_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853973.1"
                     /translation="MTRAVLLAAGLVATVVASGCQSTQNKNDELAKQAKKLTLQKGLK
                     VTRTNKHVDVVHTTVLQDANGAAAVVTLRNLTSKPLPRQPISIAVLGGGGKKLFANDA
                     PGLEPSLVNTPPIPARGTAAWIDDQLQLDGRPKKVNAKVGVASAAAKVPAREPKLIVS
                     RPTFHDDPISGIAVVGTVKNASKVEQRKLVISVIARKGGRIVAAGRGQIERLKPGKTG
                     NYKVFFIGNPRGARLSVSAPPTQFK"
     gene            complement(7011..7916)
                     /locus_tag="VF752_00100"
     CDS             complement(7011..7916)
                     /locus_tag="VF752_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX8853974.1"
                     /translation="MDQVMPPPVRRIVVDGEPTTVAPPPPRPETANGHPTGVTPLRRR
                     ARTSSPGRERMRVEELSVAYAGKLAVKEVTLPVRQGEVLALIGPSGCGKTTLLRSLNR
                     LTELTPTASRSGRILLDDADVERFEVTDLRRRVSMVFQQPNPFPMSVFDNVAYALREQ
                     GSKRPRKHDLQPAVHDALARAGLLAEVQDDLDRPALRLSGGQQQRLCIARSLAARPEV
                     LLLDEPCSALDPQSTAKIEELILELRRELAVVIVTHNLQQAFRIADHVGFMYLGHLEE
                     YGPVDQVFGTAKSKRTRDYVSGAFG"
     gene            complement(7904..8869)
                     /gene="pstA"
                     /locus_tag="VF752_00105"
     CDS             complement(7904..8869)
                     /gene="pstA"
                     /locus_tag="VF752_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX8853975.1"
                     /translation="MAAHAHAVAPRTRAVESTRSWRTLDRIGLLFCWAAGIALCLITA
                     AIVLYMLVKGVQFLRPHLLVTHPQPDLDQSKTGGFLDPILGTLILTVIGISVALPLGV
                     ATAVWLTEYGRPASLARAVESGVEIVAGTPSIVLAIFGLVVFQQHIFSWMSFTAQGNA
                     VFARSFLTAGAMMSLIALPLVVGSTREALQSIPRHVREASYALGKSRFATIRRVLLPA
                     ARPGMATGATLGMGRIAGDTAIVVILLGASLQLQAEGSTPLLKTLRGTGSTLTSYVYN
                     NSPAGEGNAPQKAYAAAFVLLLIVVALNFAVDVLVRRRKEPQWIR"
     gene            complement(8869..9858)
                     /gene="pstC"
                     /locus_tag="VF752_00110"
     CDS             complement(8869..9858)
                     /gene="pstC"
                     /locus_tag="VF752_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8853976.1"
                     /translation="MADLPGRVSGQRQDRRAELGLGALASCVLLLIVGMVVFVFGKAW
                     PSFAHNGLAWFGGGGNVDRQLTDIFNSPANPSNYVYKMHAWPLLYGTAVTTLGAVVIG
                     ITFALLSAVFIVEFAPAQLRRVLEPVVRLLAAVPSVIYGLIGILVIVPFVGNHLISQG
                     RKESVQFVVQLNGTSLLVAVLILTVMIVPIMIAIMVDALRAVPSAWTEGSAALGVNRW
                     RTIWSISVRAARPAIVAAAVLATARALGEAIMLSMVSGSVGFAPNPIDGLTFFVEPTR
                     PLAATIVDNAEGLSVKPFGQTLYAFAAVLLVSSMFLSLAGWAAKQPLRKYGNA"
     gene            complement(9858..10667)
                     /locus_tag="VF752_00115"
     CDS             complement(9858..10667)
                     /locus_tag="VF752_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8853977.1"
                     /translation="MSSKRARVAGCLTLAALAAVPAAAAAKPTVTMSGSTSVAPLAAK
                     LASAYNKQFHHSTVFKLAQGGSDIGVADVAAGRVTIGNSSRDPKPTDPGGLVFNKIAR
                     DAVCIVTSPQNKIANLSQAQVQGLFSGKISDWNQVPGSTVSGAPDVIVRTAPSGTQDA
                     FQKIFMGTAKVTTAASQKASNGLVAQAVKSDPRAVGYVSLAFAKGLNDAGYKGVACNL
                     RNAKSNQYGGVRNFWMVTRGPAKGAAKKFISWIKHSSTANRIIATGWVPLH"
     gene            complement(10799..11206)
                     /locus_tag="VF752_00120"
     CDS             complement(10799..11206)
                     /locus_tag="VF752_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8853978.1"
                     /translation="MRAGHTVPPVGTSSQAAPIRARLGGEDELLRFEELELLPSDYQV
                     RVHGVRVPLTMREFETLLALAERPGRVVMRPEIYGRVWGGEMPRRDRSVDVFVRKVRL
                     KLAVASPEWRYIHTHFGIGYRFTAERVPDGGSK"
     gene            complement(11250..13319)
                     /gene="ppk1"
                     /locus_tag="VF752_00125"
     CDS             complement(11250..13319)
                     /gene="ppk1"
                     /locus_tag="VF752_00125"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX8853979.1"
                     /translation="MTQTARTQETTETTRYANRELSWLDFNDRVLQLAEDDSTPLLER
                     AKFMAIYASNLDEFFMVRVAGLHDQVDAGIDVRGPDGFTPSEAIDAICERVRGMCTRL
                     TTLFETEIRGALAEQGIRIVSCETCDQVQLSAVDKVFREQIFPVLTPLAVGPGRPFPY
                     ISNLSLSLAVWVRDPMSGTETFARVKVPKEVLPRFMPIGDGVFVPLEDVIARHLGSLF
                     PGMEIVHHSVFRVTRDADFSVSDDADDLLRAVEDELRRRRFGEVVRLEVSAALDPELR
                     ERLIEWIDVEEREVYDVDGLLDYSDMWEIHNVEGHAELREPPWAPVTKPELLDEEREE
                     VDVFAAMRKGDILVHHPYDSFASSVERFVAQAVRDPDVLAIKLTVYRTSDDSALVPSL
                     IEAAERGKQAVCLVELKARFDERRNIGWARALEEAGAHVVHGLPGLKTHAKALLIVRR
                     EGGGVRHYVQIGTGNYHAVTARLYEDFGLFTMDPDITADVADLFNLLTGYARLSRQRK
                     VLVAPANLREPLIEQIRETAAAHQRGEHARIVMKMNSLVDRRCIDALYEAAEAGVRID
                     LIVRGICCLIPQENIRVVSIVGRFLEHSRVFQFERGDERVCFIGSADLMPRNLDNRVE
                     LVVPIQDEKLRAELEDTLQRCLADDTNAWVLDASAAWQRRRGGTRSVHRELIERTLER
                     AAAAAAD"
     gene            complement(13316..13873)
                     /locus_tag="VF752_00130"
     CDS             complement(13316..13873)
                     /locus_tag="VF752_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8853980.1"
                     /translation="MKARAVKGVNADASLAENARRIALVRLDELYSFAPKALDPTKKR
                     ALHDMRIAAKRLRYLLEVAEPALGSAAKRGAKEAKALQGLLGDIHDCDEMLLFVDDHV
                     SRLRADDSAAVHLAAADADDLDPALVRAAPNRTRYRGVESLRTWLQARRALLHQRFAE
                     RWAKLEAAGFRQKLESSLAAEDGAR"
     gene            complement(13870..14367)
                     /locus_tag="VF752_00135"
     CDS             complement(13870..14367)
                     /locus_tag="VF752_00135"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8853981.1"
                     /translation="MARAADVAGIGPDTPFREAAAVVVETRAEELFSLQENVLDTDDI
                     ERVHDMRVASRRLRAVLEVFAPCFPKKGHRAVLRDVKAIADALGERRDPDVAIDILVR
                     VEDALPTHDAAGLRGVVDELRERQQQANERLAEALRQMEETGLRGRIAVLVAHARGSV
                     EEAAA"
     gene            complement(14360..15250)
                     /locus_tag="VF752_00140"
     CDS             complement(14360..15250)
                     /locus_tag="VF752_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853982.1"
                     /translation="MPYASIDVGSNTTRLLVAEVRDGRLRELMSQRAYTRLGKSITKG
                     TDIPESKIAETAAIVERQARQARELGAREIVVVGTAAVRAAANRDDLVDAIEREAGLT
                     IRVLNEDEEATLSFIGATRTAPAPLEGTVAVIDVGGGSTEIVFGTLSDGVTWQSSFSI
                     GSGFLADSYLHSDPPSVGELEDVRRHVAGVFEGLELPQADQAVAVGGTANALRRIVGT
                     VFEHETLERAIRILASEKRDNVSRRFEIDMERVHILPGGVLIFEQLSDQMGLPLAMGK
                     GGLRDGVLLEIAANEAGAHG"
     gene            complement(15254..15694)
                     /locus_tag="VF752_00145"
     CDS             complement(15254..15694)
                     /locus_tag="VF752_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8853983.1"
                     /translation="MIWLLRHAEAADGEPDAERPLTEKGQRQARAAGRALAALEVRID
                     ACVSSPKVRAVQTAELACEPLGLEVQVDDRLAGGQFDPGEVADRRGEHVLLVGHDPDF
                     SMAVHNVTGAQVRMKKGGLAAIDKGELLVLLRPRELTAVAKAYG"
     gene            15937..16677
                     /locus_tag="VF752_00150"
     CDS             15937..16677
                     /locus_tag="VF752_00150"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX8853984.1"
                     /translation="MHFHLLPGLDDGPAELGESVELARAAAAEGTRAIVATPHVRHDY
                     VTDVSILPSLVREVQRELDSSGVGITVECGGELGADMVGVLNQDELETIAVGPRGRRW
                     LLLETPFGGIDRQFHDAAEELRDRGFAVVLAHPERCVTLFEHDSLGLRRELARGALLQ
                     VNGGSIAGVHGPGPEEAALRLISSGLVAAIASDAHSAERLPMLENAYLAMLDNDIHPA
                     RAAHVTDCAPSRLLRAGIPAHKPVPAAA"
     gene            complement(16792..17436)
                     /locus_tag="VF752_00155"
     CDS             complement(16792..17436)
                     /locus_tag="VF752_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX8853985.1"
                     /translation="MSLFRRVRKADPVLLSLLEESGRNVERTGALLRDMLADFPERAD
                     LARDVLICEQEGDRITHDIIHRLGGNGHGRGASHGPIPGDDAYELARVLDDIVDYAEE
                     AADALGVYGVEAPMEQAQQLAEVLVEAGREVSASLATLPSGEGLTPHLIEIHRLENEG
                     DRISRDAMASLFHSGTDPMIVIRWKDIFESLEEAVDSCEKVAHILEGMSLRLPH"
     gene            complement(17433..18218)
                     /locus_tag="VF752_00160"
     CDS             complement(17433..18218)
                     /locus_tag="VF752_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8853986.1"
                     /translation="MRVQPGPIRLAIVDSDSGFVRVLAKRVEEMGWQHRILSGPVRPD
                     ELVSMRVHAVVVDLALLGSSAWEFLERLCDELPALGVIVCTGQSTVAQRVRGLRLGAD
                     DWVTKPTHPQEVVARVESVVRRRKRADSSEDAGPVHAGELEIRPDQFQTFVGGQSIDL
                     TRREFELLHLLAGSEGRVLEREEVYRHVWGYTMVRGDRSVDVFVRKLRQKIERFSPDW
                     TYIHTHFGIGYRFEPVPAEGTLPPEAPADEVAAEADAELPMPS"
     gene            18381..18761
                     /locus_tag="VF752_00165"
     CDS             18381..18761
                     /locus_tag="VF752_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8853987.1"
                     /translation="MESAIQGEVLHAGALEVRPAEYSAVVAGRQLSLTVRELQLLTAL
                     TRRAERIVSREELYEEVWGRQLDKCDRSVDVYVSKLRQKLELALPGSNYIHTHFGFGY
                     RLSAGGSSPYSGSSHLFHTDATSR"
     gene            18823..19932
                     /gene="pstS"
                     /locus_tag="VF752_00170"
     CDS             18823..19932
                     /gene="pstS"
                     /locus_tag="VF752_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX8853988.1"
                     /translation="MKSRQWLAMAAASVLAIGVAACGSSNNDNAGSTGGGGGKSSGTA
                     DGAGSTFAAPIYQQWGSTLKGDGATINYQPVGSGAGIAQLTAGTVAFAGSDPPMKPEE
                     ISAAKKKGDPVHIPVAFGAITVSYNLSGVKSGLKLDGATIADIFLGKVKKWNDPEIAK
                     LNPGTSLPSSNITVIHRSDESGTTKGFTDFLQSYSSEWKSKVGSDKTVKWPTGTGAKG
                     NDGVAAAIKQTAGSIGYVEQAYALQNNFTTADVKNQSGKFVAPSLESTTAAGEGVKVP
                     SNLQFTIINSPNPAAYPIASQTFLIVYKDDCKAGVLKKADAPALGTFLNYAVGQGQSV
                     LGQLQYAKLPSAIQTKAQDAVKGLQCNGSPLGGSA"
     gene            19937..20929
                     /gene="pstC"
                     /locus_tag="VF752_00175"
     CDS             19937..20929
                     /gene="pstC"
                     /locus_tag="VF752_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8853989.1"
                     /translation="MEARSLELRGGRSLLERSPLSGIGDKILRWVLTALAAGILALIA
                     FFFIRLYVEAKPAFDRFGIFGFTFDNNWDVSRQIFGALPLVVGTLITSAVALLIGVPV
                     AVGTALYVTEFAPRPIRAVLSFTIELLAAFPSVVLGLWGVYVLIPKLKPAEQWFSDTF
                     SFLPFVGGPVAGPNYFIGGLLLAIMLIPIVTAISREVISTVPAEHKEAALALGATRWE
                     MIKMAVLPYSRAGITGAAMLGLGRAIGETIAVTLVIGNAPQIGKQLFDQGYTLAAVIA
                     NEFGEAASDPVHRAALIAAGLVLFVLTLAVNAIARLLVNRGVHGRRATKEAAPA"
     gene            20926..21792
                     /gene="pstA"
                     /locus_tag="VF752_00180"
     CDS             20926..21792
                     /gene="pstA"
                     /locus_tag="VF752_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX8853990.1"
                     /translation="MTAMPVVSARRRRSDLVARGLLATAAIVALIPLVLVLFYLVKKG
                     AGALSGTFFSSDPTGAFLGNQGGVKSAILGTIELVALATAIAVPLGVGVALYLTEYGR
                     DKPFAKVVRYFVDVMTGVPSIVFGLFIYITLVLTGVSGTGFAGWKGSVALALLMLPVV
                     TRASEVVLLLVPESLREAALALGAPRWRVVARVVLPTALPGIVTGTLLAVARAAGETA
                     PLLFTSAIVNGTNFNLNERTNSLPIQIFNDITQPNNQLVERAWGAALTLVLMILLLTL
                     VARLVSRRSRLT"
     gene            21789..22682
                     /gene="pstB"
                     /locus_tag="VF752_00185"
     CDS             21789..22682
                     /gene="pstB"
                     /locus_tag="VF752_00185"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX8853991.1"
                     /translation="MTYERAKDAEQEAGTVPTPFPRSQQPRTESSATRTSAYGAARPS
                     GTLARNVSVRGLNAWYGDNHAVKNLNFEIEPNKATALIGPSGSGKSTVIRCINRMHEE
                     IPGARADGEVLLDEVNVYDPKIDVVAIRRLVGMVFQKPNPFPTMSIFDNVAAGLRLTG
                     TKGVGLRERVEKSLRAAGLWEEVKDRLKEPGISLSGGQQQRLCIARTIAIEPEVILMD
                     EPASALDPLSTLRIEELVDELKARYTIVIVTHNMQQAARVADSTAFLLDGELIEHAPT
                     EKMFTNPDDERTERYVTGKFG"
     gene            22682..23374
                     /gene="phoU"
                     /locus_tag="VF752_00190"
     CDS             22682..23374
                     /gene="phoU"
                     /locus_tag="VF752_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX8853992.1"
                     /translation="MQETRVHFQEELAQLESQALGGLDLVVEALNRVMEALEHQDVEL
                     ATMVIADDDRIDGRYLEVHQGILSLLARQAPVATDLRLVAALLDVVKHMERMGDQCVN
                     IAKMLPVAGNEPPVEEEILKRLLKMGRQAAFQVAQAKQAFASRDVALAEDLVIQDDVI
                     DRLNREIFREVLDCSGDADTREWSLLMMLVARSIERIGDNAVDIGEQTAFVVTGLFRE
                     FQDASHPDLNSE"
     gene            23471..24904
                     /locus_tag="VF752_00195"
     CDS             23471..24904
                     /locus_tag="VF752_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX8853993.1"
                     /translation="MRRLFLLVAVLAAAAIAVPLSLGSSHREAPLTALDPTGDDTDVY
                     AFTANDAPGAITVVSNWIPFEDPAGGPNFYRFDDRAHYYINIDNTGDGVADVRYQFKF
                     KTKTLNKNSFLYAFPGVSSIRDSKLNVQQSYTVTREKYKNGKLKSAKVVADNLPVAPN
                     NVGPKTIPNYDAVANQAIQSVKGGGKVFAGQRDDPFFVDLGSTFDAINLRMGTGNAGG
                     GKDDLAGYGVHSIVLQVPESKVTRDGKPVSGPAASNAVVGVWSSTERPRLQVNGSANA
                     AKKKRHSRRSQDENFVQVSRLGNPLINEVVIPLGQKDRFNRTQPKDDAKNFGQYALNP
                     ELAKLMNQLFHVNAPETNRTDIVQALLTGIPGKTMISKDAVPADTLKLNLGVPPTSTP
                     NRFGVLAGDLAGFPDGRRLADDVTDIELRVIAGFLKDNKVPLGDGVDQNDKPFLSTFP
                     YVAAPTSGFDSTLKRLEPPHEPTPAQP"
     gene            24968..>25542
                     /locus_tag="VF752_00200"
     CDS             24968..>25542
                     /locus_tag="VF752_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8853994.1"
                     /translation="MTLVRHKLTRTIAAAALAFAAALAVFMLMNHSSHPARSSADSSA
                     SPASLVGASTDTRIRSFQALVRAHPRDSRGYSFLGDAYLQKVRETGDASFYTRADGVY
                     GRALELNPRDAAATTGMGTLALARHDFRAGLRYGLRAHALAPAVVKPLGVIVDAYVEL
                     GRYHQAADALQRMIDLKPNLASYARVSYFREL"
BASE COUNT         4031 a         8918 c         8623 g         3970 t
ORIGIN      
        1 accgggttgt acgtcgcgac cgtacggaac caatcgatct cgatcaggtt ccgcggcagc
       61 gccatcgacg acaggaacag caggacgaag aagagcggga acatgccctg taccgcctcg
      121 cccgatccgg tgcggagcgc cgccagtgct cccagcgttc cgaacccgag cgtgatgaga
      181 acggacagcg cgagcagcac gaggacgcct ggggcgccgg cagcgaagcc ggcgccggca
      241 gcctcgccga ccgcaagaaa gatgacggcc tgggcgattc cgagcacgat cacaccggcc
      301 agctgaccac cgagcagggc gccccgggtc agcggcgtca gcgatagccg ggagagaaaa
      361 ccgctctcga tgtccatggc gaagttggtc ccggcgctgt tcgcggcgaa gagcgcgccc
      421 tgcacgaacg gcacggcgag cgcgaacgtg atgtacgagt cggccgggaa cctcgggatc
      481 ttggtggcgg cgtcgaggcc gccggcattt accgccagca gaagcagcgg gaacgcgatg
      541 gagaacacga tgttggccgg ctgccggaga agccggatga ccgaccggcg cacgagcagc
      601 cccgcgtcgg cgagtccgct cacgcagagg cctccaccgg cgctcccctg acccgaccgg
      661 gctcgctgac cgactcgtct tcctcctcgc cggccccctc gagcgagcgg ccggtcttct
      721 cgaggaacac gtcgtcgagg ctcggttcgt gaaccgacag acgggcgacg cggatttccg
      781 cggcgtcgag ggcacggacg acgtcggcga gctcgtcgcc gtttccgaac cgtgtggcca
      841 ccccgtcgcc agaagtctcc gagcagtcgc cgaagcgctc gagtacgccg gccaaccgct
      901 cccgatcgcc ggggtcggcg ggtacgacct caatcgagga gcgaccgatc tccgccttga
      961 gctgtgccgg cgtgccttcg gcgacgatcc gcccgtggtc gatgatgccc acgcggtcgg
     1021 cgagcacgtc cgcctcctcg aggtactggg ttgtcaggaa gatcgtcaca ccctcctcct
     1081 ttgacagccg cgagacctcg tcccagagcg cgctacggct ctggatgtcg aggcccgttg
     1141 tgggctcgtc gaggaagagg atccgggggc ggtggaccag tgccaagccg aggtcgaggc
     1201 ggcgcttcat gccgcccgag taaccgccga ccttacggtc ggcggcttcc gtgagaccca
     1261 cgcgcgtgag cagctcgttg ctgcgcgcct cgcggtctgc cttgctgagc ccgtgcaggc
     1321 ccgcctggag ccgcatgtgc tcgcgccctg tgaggtacgg gtcgagcgcc gcatcctgga
     1381 gcgctgcgcc gatcgaccgg cggacggccg cgccgtcttc gacgatgtcg tgaccggcga
     1441 ctcgcgcccg tcccgctgtc ggagggagca gcgtggtgag catcagcacg gtcgtcgact
     1501 tgccggctcc gttggggccg aggaagccgt agatctcgcc cggcgcgacc tccaagtcga
     1561 tgccgtcgac cgcgcgcggg cctttcttga atacgcgtac gaggccctcg acctctatgc
     1621 cgttcgcttc gctcatggac cgcaaggtag cacaactatt gaaggcttca atcgttctat
     1681 aagttgctct atactcgctt acgtgggaac cgcgaccacg aaggcgcaaa ccgagcccgc
     1741 gagtgaagcg tgggagctgt tcatggagct cttcggcacc gtccgcccgc ggatgctcga
     1801 catccaggcg gagtacggcc tcaagccgcc gcagttcttc gcgctcgacg cgctcgacga
     1861 gcccgcgcct atgagcagca tcgccgacct ccttcgctgc gaccgctccg cggtgacctg
     1921 gatcaccgac cggctcgagg agcgcgggta cgtggagcgg cgaagcgacg ggcgcgaccg
     1981 ccgcgtcaag ctcctagccc tcacagacga gggcaggcga gtacgcgagg agattcgcgc
     2041 tcggctggcc atcccacccg aggccctcac gcgcctctcg cccgccgagc agcgcgctct
     2101 gcgagacctg ctgcgcaagg cactcgagcg cgagtgacgc gttcgctgcc ggacttgatg
     2161 ccgaggaccg cgacaacgaa gaagccccgc cgaagcgggg cattcctcta gcgcgagcga
     2221 cgagactcga acctctaggc gagcgccttt gccttgatcg cctcgaactc ctgctgggtg
     2281 atcgtgccgt tgtcgagcag ctgcttggcc tgcgcgatct cggatgcgga agaacgaccg
     2341 ctcgccgcgt cgcgcacgta ctcatcgaac tgctgcttct gggcctgcgc ctgcctcacg
     2401 ctgcgctcgc gcatgccgtc gtgctgggcg atcagataga tcagcacgcc gagccacggg
     2461 aggaccacga cgaagatcac ccatccggcc ttggcccaac cgccgatgtc gtcccggcgg
     2521 aagatgtcga tcagcacggt gacgaggacc cagatccagg ccacccacag gaagaagatg
     2581 aggaccgtcc agaacacgtc caggaacgga tagtcggccg caatcggtaa cacgcgagtc
     2641 tcccctcgat cgccggtgag tcctggtcag atcgtcgcgc atgtattgga ctgccgctag
     2701 tgcgtgtcgg gctaggccgc gcgggccccg tccgcgatcc ggatgtggtg ctgagagcag
     2761 aacgccccgc cgaagcgggg cattcctcta gcgcgagcga cgggactcga acccgcgacc
     2821 tccggcgtga caggccggcg ttctaaccaa ctgaactacg cccgctgagc gcccctacgt
     2881 tagcgaagcg ccggggcgag cggctacctg ctcttcgccg cctttttcgt cgcagcggcc
     2941 tccgatcgcc ggtacttctt cgtgcatccc ttgggcgcct tgagcgggaa cggaccgaag
     3001 gtctggccgt tggcgctcga cacgatcagc gacgcggtca gtgcgcccgc ggacgacctg
     3061 tcgtagacgc aggggctcac gtcgaagccg attccactgc gtacgaaata gcccgccttg
     3121 atcaggtcct tggcgttgtc cgcggcacag ctgatcccgc ggccgggaat gtcgccttcg
     3181 cacgacgtga ggacgcccgg cgcatgatcc tgcggcgcga acgacatcgc ctgcacaccg
     3241 aagacgtcga tgtcgcggtt cgtcacgacc gtgaatccgc gcacgttctg cgagcaggcg
     3301 aacgcaatac cgatcgcgtt gtccgaatcc acgtcggctt tgagatcgcc actgagcgcg
     3361 ccccagtggc ccttgcagga gatcggggaa gccgccgccg tcccgggcac gagtgcgagc
     3421 gctgtaagcg cgcacaccgc gaccagagcg gccagcgcat gcccgcgcct gcctctacta
     3481 ctcgtccctt cagaacctgt catcgatcct ccgcttgttg gctggtcccc gccggcggca
     3541 tcctccgctc caggctgtga gtccggtgtg aagatgccgt gacgcccttg tgaaacagcg
     3601 ctgtcaggtc cggcagccgc ttgtcgcccg cggcggccag gcgcttcagt ccgagcagct
     3661 cttccacgtc gccgagcagc gcgtagtggt cgaacggctt gtcactggtc gtccccgcgt
     3721 cgagcagtgg cgagagcagc agcgcgccgg tgcgcttgtc ccgcgcgccc gcgggggcgt
     3781 cgtcgaacac gatcgccagc aggccgtcgt cggtgtaggc cggcgagtcg aggatcttcg
     3841 gcacccactg cttcaggaag gcgtcggcct gcggaaggcc gccgggccgc ccgtccacac
     3901 agggcacagc ccagccgccg tcgcagaggt tcggcacgat cagcgaccag ttcgcggcag
     3961 tgtcggccga ggcgaggtcg gcctccagtg agtcgaggcc gacatcgcgc acgccgcagt
     4021 cggggccgtc gagcaccgag tggaaccaca cgagcgggtt gtgggcgagc gcgtagccgt
     4081 cgccttgccg ttccgtcgtc gtgtcgtcgg gcgagccctc ggccgggtgg cggcaggccg
     4141 tgatcgcggg gtcgggcccg cgctcgaggt cgcccgcgta gaccttccaa gacaggccgg
     4201 cgtcgccgac ctggtcggcg atcgtcttcg tctttgccgg gaagaggcaa tcgccgccgt
     4261 agaccgcgca gcccgcgcgc gttgcgtccg tgggcttctg ccccgacacc agggcgtcga
     4321 agccggcgat gctcgagacc ccgagacccg agtactgcgt gaggagcact ccctgcggcg
     4381 cgagctcgtt cgcgaggtat ggcgctatcg acgccggccc gaaggccgcg tcatacgacg
     4441 cgccggcgag cacgactgtg aacacgtgct tgatgcccga gctcggcgca ggcgccggcg
     4501 tgtcgtctgg ctgcgtcgtg tccggcgcag gcgcgggcgc ctcctgcggc gccggtgcgg
     4561 tcgcggcgtt tccgccgaag ctcgacacgg cctccacccc gtgccgctca cgcgaggtgc
     4621 tggaaccacc cgccgagaga tcctggttgc cgcccgaggg cgcggagtcg gcctgtggcg
     4681 cagccgccgg ctgcgtcgcg ggcgggggca ggagcaccac ctgccgggtg ttcgcgagcg
     4741 atccgctgcc gcctgcggcg ccgccgatca cgatgccgag cgtgagcacg ccgaccacga
     4801 gcgccgctgc tacgcgaggg ttcggcagcg ctcgtaccgg cagcgagcca gtctccgcga
     4861 cgggctccgg ctctgccgcc gcgcgaacgg gctcgcgcgc gcccggctgg cgggcgccgc
     4921 actcgaggca ccagccctgg cccggcccga gcggcgctgc gcagtcgccg caacggctca
     4981 tgccgcggga gtcacctcca tgagggtcgt cccgcactgc cagcagtaga cggcgccgcg
     5041 gctgtgcagc gcgccgcacg atgggcagct gcccgtcgct cccgcgtcct ccatcttcag
     5101 cagccgctcg atctcgccga gctcggcgtc gacctcctga aggcgcgccg cgcggcgcac
     5161 cagcacgtcc aaccggaagt gatcgcggat cgccatctcg taggtgaggc cgccgagatc
     5221 ccactggagc tcggcgagct cgcgcgcgag gcgttccctg cgggcggcga gctccgctcg
     5281 tctcgtgtcg ggctgctcgg ccgcgggcgg cgctgcgggt cgcgccgccc tgcggccggg
     5341 cagccggtcc cggagtctgg ccaccgcgct cacttgatgg tctccgtcgg cgagccgccg
     5401 cccacgggct tgctcttgtc gaccggcggc ggcttcccgg gcagcttgag cgtggacggc
     5461 agcttcttcg actgcttctg gtactggtcg gggctcaggt tgttcagctg ctggagctgt
     5521 ttcttgttcg cggtcgccga ctccttgccg gacagcgact tcacgttctt gcccccgctc
     5581 gcgcccgcgc tgtcgcccac ctgcaccacg cgcgcgtccg ggaacttctt cttcaggccc
     5641 gcgagcgcct tctgagcctg cttcttcgtg tcgaacacgc ccgagtagac gacgtagaag
     5701 ccgcccttca ggctcgcgtg gctgtcggag tcgagcgcgc ccacgtcctt ggcccccttc
     5761 gacgtggcgg cctgcttggc ggccgccacc tgtgaagcgc cgttgccgct cttcggcagc
     5821 gcctgcacct gcacggtcca gccgctcttc ccggacggcc agtcctcctt gaaagcggcg
     5881 gccggcgttg cgcccccgct cgcggcgccg cccgccgcgc cgccgacggt gatcacctgc
     5941 ggagagccgc tcgacttgcc gcccgacgtg gtgttcccga tcagcactcc catggcaagc
     6001 gccaccacga gcacggcggc gcccccggcc gcgattggcc acgtgacagg caggccgccc
     6061 ggcggcggcg cggcaggctg cgccggccgg ccttgagaag gaggccctgc cggaggagcg
     6121 gacaaggcgc gttcgtaatc cacgcgcggc tccgcgcggc ggagcccgca gttgaggcag
     6181 taacgctggt ccggggcgag cggcgcgcca cagttgccgc agctctcgcc cggctctccc
     6241 agccgcggca gctcaagggt ctcgggtgtg ctcatgcctg cctcacttga actgggtcgg
     6301 cggggcgctc acgctgagcc gggccccgcg ggggttgccg atgaagaaga ccttgtagtt
     6361 gcccgtcttg ccgggcttga ggcgctcgat ctgacctcgg ccggcagcca cgatccggcc
     6421 gcccttgcgg gcgatcaccg agatcacgag cttgcgctgc tccaccttcg aggcgttctt
     6481 gacggtcccg acgacggcga tgccgctgat cgggtcgtcg tggaaggtcg ggcgcgagac
     6541 gatcagcttc ggctcgcgtg cgggcacctt tgccgccgct gaagcgactc cgaccttcgc
     6601 gttcaccttc ttgggccgac cgtcgagctg cagctgatcg tcaatccacg cggcagtgcc
     6661 gcgcgcagga atcggtggcg tgttcacgag cgagggctcg agtccgggag cgtcgttggc
     6721 gaacagcttc ttaccgccgc ctccgagcac ggcgatggag atcggctgac gtgggagcgg
     6781 cttgctcgtg aggttgcgca gcgtgacgac cgcggctgcc ccgtttgcgt cctggagcac
     6841 cgtggtgtgc accacgtcga cgtgcttgtt cgtgcgcgtg accttgaggc ccttctgaag
     6901 cgtgagcttc tttgcctgct tggcgagctc gtcgttcttg ttctgggtgg actgacagcc
     6961 actcgcgacg acggtcgcga cgagcccagc ggcgagcagc acggcccgcg tcatccgaac
     7021 gccccgctca cgtagtcgcg cgtgcgcttg ctcttcgcgg taccgaagac ctggtcgacg
     7081 gggccgtact cctcgaggtg gccgaggtac atgaagccca cgtggtccgc gatccggaac
     7141 gcctgctgga ggttgtgggt gacgatcacg accgcgagct cgcgccggag ctcgaggatc
     7201 agctcctcga tctttgccgt ggactgcggg tcgagcgcgg aacagggctc gtcgagcagc
     7261 aggacctccg ggcgagccgc cagcgaccgc gcgatgcaga ggcgctgctg ctggccgccg
     7321 gacaggcgca gggcgggacg gtcgagatcg tcctggacct ccgcgagcag gcccgcgcgc
     7381 gcgagcgcgt catgcaccgc aggctgtaga tcgtgcttgc gcggccgctt cgatccctgc
     7441 tcgcgcagcg cgtacgccac gttgtcgaac acgctcatcg ggaacgggtt cggctgttgg
     7501 aacaccatgc tcacgcgacg gcgcaggtcg gtgacctcga agcgctcgac gtccgcgtcg
     7561 tcgagcagga tgcgcccgct ccgggatgcg gtcggtgtaa gctcggtgag ccggttgagc
     7621 gagcgcagca acgtggtctt gccgcagccc gaggggccga tcagcgcgag cacctcgccc
     7681 tggcggacgg gcagcgtgac ctcctttacg gcgagcttgc cggcgtaggc cacgctcagc
     7741 tcctcgactc gcatgcgctc acggcccggt gaggaggtcc gtgcccggcg cctgagcggc
     7801 gtgacaccgg tcgggtggcc gttcgcagtc tctggtcgcg ggggcggtgg cgcgaccgtc
     7861 gtcggctcgc catccaccac gatgcgccga acgggcggcg gcatcacctg atccattggg
     7921 gctccttcct tctccggacc agcacgtcaa cggcgaagtt gagggcgacc acgatcagca
     7981 gcagcacgaa tgccgcggcg taagccttct gcggcgcgtt gccctcgccg gcgggagagt
     8041 tgttgtagac gtagctcgtg agcgtgctgc cggtcccgcg cagcgtcttc agcagtgggg
     8101 tcgagccctc cgcctgaagc tggagcgacg cacccagcag gatcacgacg atcgcggtgt
     8161 cgccggcgat gcggcccatc ccgagcgtcg cgccggtcgc catgccgggt cgagcggccg
     8221 gcagcagcac tcgccggatc gttgcgaagc ggctcttccc gagcgcgtac gaggcctcac
     8281 gcacgtgtcg cgggatcgac tgcagcgcct cgcgcgtcga gccgacgaca agcggcagcg
     8341 cgatcagcga catcatcgcg cccgcggtga ggaacgagcg cgcgaacacc gcgttgccct
     8401 gagccgtgaa cgacatccag ctgaagatgt gctgctggaa caccaccagg ccgaagatcg
     8461 cgagcacgat gctcggcgtg ccggcaacga tctccacgcc cgactcgacc gcgcgcgcga
     8521 gcgaggcggg ccgcccgtac tcggtgagcc acaccgcggt ggccacgccg agcggcagcg
     8581 cgaccgagat gccgatcaca gtgaggatca gcgtgccgag gatcgggtcg aggaagccgc
     8641 ccgtcttgct ctggtcgaga tccggctgcg ggtgcgtgac cagcaggtgc ggccgcagga
     8701 actgcacgcc cttcacgagc atgtagagca cgatcgccgc ggtgatcagg cagagggcga
     8761 tgccggcggc ccagcagaac agaagtccga tgcgatcgag cgtgcgccac gagcgggtgg
     8821 actcgactgc ccttgtgcgc ggggcgacgg cgtgtgcgtg cgcagccatc aggcgttccc
     8881 gtacttgcgc agcggctgct tggccgccca cccggcgagc gaaaggaaca tgctcgacac
     8941 gagcaggacc gccgcgaacg cgtagagcgt ctggccgaag ggcttcacgg acagcccctc
     9001 cgcgttgtcc acgatcgtcg ccgcgagcgg gcgtgtgggc tcgacgaaga aggtgaggcc
     9061 gtcgatcggg ttgggggcga atccgaccga ccccgagacc atcgagagca tgatcgcctc
     9121 gccgagggca cgcgccgtgg cgagcaccgc ggccgccacg attgcggggc gcgcggcgcg
     9181 caccgagatc gaccagatcg tccgccagcg attcacgccg agcgccgcag agccctcggt
     9241 ccacgcgctc gggacggcgc gcagcgcgtc gaccatgatc gcgatcatga tcggcacgat
     9301 catcaccgtg aggatcagca cggccacgag cagactcgtg ccgttcagct gtaccacgaa
     9361 ctgcacggac tccttgcggc cctggctgat caggtgattg ccgacaaacg gcacgatcac
     9421 gaggatcccg atcagtccgt agatcaccga cggcaccgcc gccaggagcc gcacgacggg
     9481 ctcgagcacc cggcgcagct gagccggcgc gaactcgacg atgaataccg ccgacaggag
     9541 agcgaaggtg attccgatca cgaccgcccc gagcgtcgtg acggccgtgc catacagcag
     9601 cggccaggcg tgcatcttgt agacgtagtt gctcgggttc gcgggcgagt tgaagatgtc
     9661 ggtgagctgg cggtcgacgt ttccgccgcc gccgaaccac gccagcccgt tgtgggcgaa
     9721 cgacggccac gccttcccga agacgaagac caccatgccc acgatgagga gcaggacgca
     9781 ggacgccagc gcgccgagcc ccagctcggc gcgccggtcc tgcctctggc ccgacactcg
     9841 gccgggcaag tcggccatca gtggagcgga acccatcccg tcgcgatgat tcggttcgcg
     9901 gtcgagctgt gcttgatcca gctgatgaac ttcttcgcgg cgcccttcgc gggaccgcgg
     9961 gtgaccatcc agaagttccg gacgccgccg tactggttcg acttggcgtt gcggaggttg
    10021 caggcgacgc ccttgtagcc ggcgtcgttc aggcccttcg cgaacgccag gctcacgtag
    10081 ccgacggcgc gtgggtcgga cttgaccgcc tgcgcgacca ggccgttcga agccttctgg
    10141 gaagccgcgg tcgtcacctt cgcggtgccc atgaagatct tctggaacgc gtcctgggtt
    10201 ccagacggag cggtgcggac gatcacgtcg ggcgcgccgc tcaccgtcga tcccggaacc
    10261 tggttccagt cgctgatctt gccgctgaac agcccctgaa cctgggcctg cgagaggttc
    10321 gcgatcttgt tctgcgggct cgtgacgatg cagacggcgt cacgggcgat cttgttgaac
    10381 accagtccgc ccggatctgt cggcttcggg tcgcggctcg agttgccgat cgtcacacgc
    10441 cccgcggcca cgtcggcgac gccgatgtcg gatccgccct gggcgagctt gaacacggtg
    10501 gagtggtgga actgcttgtt ataggcactc gccagctttg ccgcgagcgg ggcgaccgag
    10561 gtcgatccgc tcatggtcac cgtcggcttc gccgcggcgg cggccggcac ggcggcaagt
    10621 gcggccagcg tcaggcagcc cgcgacgcga gctcgtttgg aggacaacac gactcctttc
    10681 gagagaacta agggcagcgc tcgccgctgc ccggcggcgc ggcgcgagta tccgcgcgca
    10741 ggcgccgcct ccgcgttaag gaggcgtgca tcggtggaga aaaagtggat aaggcgcctc
    10801 acttcgagcc gccatccggt acgcgctcgg cggtgaagcg gtagccgatc ccgaagtggg
    10861 tgtggatgta gcgccattcc ggtgacgcca ccgcgagctt cagccgcacc ttgcgcacga
    10921 acacgtcgac cgagcgatcg cggcgcggca tttctccgcc ccatacgcgc ccgtagattt
    10981 ccgggcgcat caccacccgg ccgggcctct cagcgagcgc gagcagcgtc tcgaactcgc
    11041 gcatcgtcag cggcacacgg accccgtgca cccttacttg gtagtcggag ggaaggagct
    11101 ccagctcctc gaaacgcagc aactcgtcct cgcccccgag ccgcgctctt atcggcgcgg
    11161 cctgcgaact cgtgcccacg ggcggcacgg tatggcccgc gcgcatcgcg gctcgtgaag
    11221 aagtggtgat cgtttggaga aagccgcgct cagtcggccg ctgcggctgc ggcgcgctcg
    11281 agcgtgcgct cgatcagctc acggtgcacg ctgcgggtgc cgccgcgcct gcgctgccag
    11341 gcggccgagg cgtcgagcac ccaggcgttc gtgtcgtcgg cgaggcagcg ctgaagggtg
    11401 tcctcgagct ctgcgcgcag cttctcgtcc tggatcggaa cgacgagctc gacacggttg
    11461 tcgagattgc gcggcatgag gtcggcggat ccgatgaaac acacgcgctc gtcgcctcgc
    11521 tcgaactgga acacgcgcga gtgctcgagg aagcgcccca cgatcgacac cacgcggatg
    11581 ttctcctgcg ggatgaggca gcagattccg cgcacgatca ggtcgatccg gacgccggcc
    11641 tcggcggcct cgtagagcgc gtcgatgcag cggcggtcca cgagcgagtt catcttcatc
    11701 acgatgcgtg cgtgctcgcc gcgctgatgc gccgcggccg tctcgcgaat ctgctcgatc
    11761 agcggctcgc gcaggttggc gggagcgacg agcaccttgc gctgacggct gaggcgcgcg
    11821 tagccggtga gcaggttgaa caggtccgcc acgtcggcag tgatgtccgg gtccatggtg
    11881 aagagcccga agtcctcgta gagccgcgcc gtaacggcgt ggtagttgcc ggttccgatc
    11941 tgcacgtagt gacgcacgcc gccgccctcg cgacgcacga tcaggagcgc cttcgcgtgt
    12001 gtcttcaggc cgggtagccc gtgcaccacg tgcgcgcccg cctcctccag cgcgcgcgcc
    12061 cagccgatgt tccggcgctc atcgaagcgc gccttcagct ccaccaggca cacggcctgc
    12121 ttgccgcgct cggcggcctc gatcagcgac ggcacgagcg cggagtcgtc ggacgtccgg
    12181 tacacggtga gcttgatcgc gagcacgtcg gggtcgcgca cggcctgcgc cacgaagcgc
    12241 tcgacgctgc tggcgaacga gtcgtacggg tgatgaacga ggatgtcgcc cttgcgcatc
    12301 gcggcgaaca cgtccacctc ctcgcgctcc tcgtcgagca gctcgggctt ggtgaccggc
    12361 gcccagggcg gctctcgcag ctcggcgtgg ccctccacgt tgtggatctc ccacatgtcg
    12421 ctgtagtcga gcaagccgtc cacgtcgtag acctcgcgct cctccacgtc gatccactcg
    12481 atgagccgct cgcgcagctc gggatcgagg gcggccgaga cctcgagccg cacgacctcg
    12541 ccgaaacgcc gccgccgcag ctcgtcctcg accgcgcgca gcaggtcgtc ggcgtcgtcg
    12601 gacacgctga agtcggcgtc gcgggtcacg cggaacacgc tgtggtgcac gatctccatt
    12661 cccgggaaga gcgacccgag gtggcgggcg atcacgtcct cgagcggcac gaacacgccg
    12721 tcgccgatcg gcatgaagcg cggcagcacc tccttcggca ccttcacgcg cgcgaaggtc
    12781 tcggtgcccg acatcgggtc gcgcacccac acggcgagac tgagcgagag gttcgagatg
    12841 tacgggaacg gccgccccgg gccgaccgca agcggcgtca gcacggggaa gatctgctcg
    12901 cggaagacct tgtcgactgc ggacagctgc acttggtcgc aggtctcaca cgacacgatc
    12961 cggatgccct gctcggcgag cgctccgcgg atctcggtct cgaagagggt ggtgagccgt
    13021 gtgcacatgc cgcgcacccg ctcgcagatc gcgtcgatcg cctctgacgg cgtgaacccg
    13081 tccggcccgc gtacgtcgat tccggcgtcc acctgatcgt gcaggcccgc gacgcggacc
    13141 atgaagaact cgtccaggtt cgacgcgtag atcgccatga acttcgctcg ctcgagaagc
    13201 ggcgtggagt cgtcctcggc cagctggagc acgcggtcgt tgaagtcgag ccaggagagc
    13261 tcgcggttcg cgtaccgcgt ggtttcggtc gtctcctggg tgcgtgccgt ctgcgtcatc
    13321 gagcgccgtc ctcagcggcg agcgagctct cgagcttctg gcggaagcct gccgcctcga
    13381 gcttcgccca gcgctccgcg aagcgctggt ggagtaacgc gcggcgggct tgcagccacg
    13441 tcctcaacga ctctactcct cggtagcggg tgcgattcgg ggccgcccgc acgagcgccg
    13501 gatcgaggtc gtcggcgtcc gcggccgcca gatgcacggc cgcggagtcg tccgcgcgca
    13561 gccggctcac gtggtcgtcc acgaacagca gcatctcgtc gcagtcgtgg atgtcgccga
    13621 gcagcccctg cagcgccttc gcctccttcg cgcctcgctt ggccgccgaa ccgagcgccg
    13681 gctccgccac ctcgagcagg tagcgcagcc gcttggcggc gatgcgcatg tcgtgcagcg
    13741 cgcgtttctt cgtcgggtcg agcgccttcg gcgcgaacga gtacagctcg tcgagccgca
    13801 cgagcgcgat acggcgcgcg ttctcggcta gcgaggcgtc ggcgttcacg cccttgaccg
    13861 cgcgcgcctt caagccgccg cctcctccac gctgccgcgc gcgtgggcga cgagcaccgc
    13921 gatgcgaccg cgcaggccgg tctcctccat ctgtcgaagc gcctcggcga ggcgctcgtt
    13981 cgcctgctgc tggcgctcgc gcagctcgtc cacgaccccg cgcagccctg ccgcgtcgtg
    14041 tgtgggcagc gcgtcctcga cccgaaccag tatgtcgatg gccacatcgg ggtcgcgacg
    14101 ttcgccgagc gcgtccgcga tcgccttcac gtcgcgcagc acggcgcggt gccccttctt
    14161 cgggaagcag ggcgcgaaca cctcgagcac cgcgcgcagc ctgcggctcg cgacgcgcat
    14221 gtcgtgcacg cgctcgatgt cgtcggtgtc gagcacgttc tcctgcagcg agaagagctc
    14281 ctcggcacgc gtctcgacga cgacggccgc ggcctcgcgg aatggtgtgt cgggaccgat
    14341 cccggcgaca tcggctgccc tagccatgcg cgccagcctc gttcgcggcg atctcgagaa
    14401 gcacgccgtc gcgcaggccg cccttcccca tcgcgagcgg cagacccatc tggtcggaca
    14461 gctgctcgaa gatcagcacc ccgccgggaa ggatgtgcac acgctccatg tcgatctcga
    14521 agcgccgcga cacgttgtcc cgcttctcac tcgcgagtat tcgtatggcg cgctcgagcg
    14581 tctcgtgctc gaacaccgtg ccgacgatcc ggcggagcgc gttggcagta ccgcccacag
    14641 ccaccgcctg atcggcttgc ggaagctcga ggccctcgaa gacgcccgcc acgtgccgcc
    14701 gcacgtcctc gagctcaccc acggacggcg ggtcggaatg caggtacgag tcggcgagga
    14761 agcctgaccc gatcgagaac gagctctgcc acgtgacacc gtcggacagc gtcccgaaaa
    14821 cgatctcggt ggagccgccg cccacgtcga tgacagcgac ggtcccctcg aggggcgccg
    14881 gcgccgtacg cgtcgcgccg atgaacgaca gcgtggcctc ctcgtcctcg ttgagtactc
    14941 gaatcgtgag tccggcctcg cgctcgatcg cgtccaccaa gtcgtcgcga ttcgcggcgg
    15001 cgcgcacggc cgcggtaccg accaccacga tctcccgtgc cccaagctcg cgcgcctggc
    15061 gagcctggcg ctccacgatc gccgcggtct ccgcgatctt cgactccgga atgtcggtgc
    15121 ccttggtgat cgacttgccg agccgcgtgt aggcgcgctg gctcatcagc tctcgcagcc
    15181 tgccgtcccg aacctcggcc accagcaggc gcgtcgtgtt gctcccgacg tctatcgagg
    15241 cgtacggcat cgcctaaccg tacgccttgg caaccgcggt caattcgcgc ggccgcaaaa
    15301 gtacgagcag ctcgcccttg tcgatcgccg cgaggccgcc cttcttcatt cgcacctgcg
    15361 cgccggtgac gttgtgcacc gccatcgaga agtcggggtc gtgcccgacg agcaggacgt
    15421 gctcgccacg gcggtcagcg acctcgccgg gatcgaactg gccgccggcg agccggtcat
    15481 cgacctgcac ctccaacccg agcggctcgc aggcgagctc cgcggtctga acggcgcgga
    15541 ccttcgggct cgagacgcat gcgtcgatac gtacctccag cgcggcgagt gcgcgccccg
    15601 cagcgcgtgc ctgccgctgt cccttctcgg tgaggggcct ctccgcgtcg ggctcgccgt
    15661 cagcggcctc ggcgtggcga agaagccaga tcatgagcgc tcgaagatac ggacgggccg
    15721 ggttccagat gtgaacgaga tgtttacggc cgatcttgtg acgagccggt gaacacctcg
    15781 ttacggggca gtttacgact cgttcgaagg cccgactgcg aaccatggcg ccttcacccg
    15841 gcctgctcgc gaccgcgtga acgtgcagtg aagtcaccgc ttcttcacaa aaagtcctgc
    15901 tatgccgacg aatctctaag gcatatgaga accgatgtgc atttccattt gctgccgggc
    15961 ttggacgatg gcccggctga gctcggcgag agcgtcgagc tcgcccgcgc ggcggccgca
    16021 gagggcaccc gcgcgatcgt ggcgacgccg cacgtgcgcc acgactacgt gaccgacgtg
    16081 agcatcctcc ccagcctcgt gcgcgaggtg cagagggagc tcgactcgag cggcgtcggc
    16141 atcaccgtcg aatgcggcgg cgagctcggc gccgacatgg tcggcgtgct caaccaggac
    16201 gagctcgaga cgatcgcggt tggcccgcgg ggccgccgct ggctcctgct cgagacgccg
    16261 ttcggcggca tcgaccgcca gttccacgac gcggccgagg agcttcgcga ccgcggcttc
    16321 gcggtggtgc tcgcacaccc cgagcgctgc gtgacgctct tcgagcacga ctcgctcggg
    16381 ctccgccgcg agctcgcgcg gggcgcgctg ctgcaggtga acggtggctc catcgcaggc
    16441 gtccacggac cgggtccgga agaggcggcg ctacggctga tcagctctgg gctggtggcc
    16501 gcgatcgcct cggacgctca ctcggcggaa cggctgccga tgctcgagaa cgcctacctg
    16561 gcgatgctcg acaacgacat ccaccccgcc cgagcggcgc acgtgacgga ctgcgctccg
    16621 agccgcctgc tgcgcgctgg aataccggcc cacaagccgg tccctgccgc cgcctgagtc
    16681 gccagctcgg cggtggccga ggcgatcttt gaagactttt gggggctata gacccccaaa
    16741 gtcttcaatc ttcgctcggg ccctctcgtt actgcctgcc gttggcggcg gtcaatgcgg
    16801 cagccggagg gacatgccct caaggatgtg ggcgaccttc tcgcacgagt cgaccgcttc
    16861 ctcgagggac tcgaagatgt ccttccagcg gatcacgatc atcgggtccg tgccgctgtg
    16921 gaagagcgac gccatcgcgt cgcgcgagat gcggtcgccc tcgttctcga ggcggtggat
    16981 ctcgatcagg tgcggggtga gcccttcgcc cgacggcagc gtcgccagcg acgccgatac
    17041 ctcgcgcccg gcctcgacga gcacctcggc gagctgctgc gcctgctcca tcggcgcctc
    17101 gacgccgtag acgcccaggg cgtccgccgc ctcctcggcg tagtcgacga tgtcgtcgag
    17161 gacgcgcgct agctcgtagg cgtcatcgcc ggggatcggc ccgtgcgacg cgccccggcc
    17221 gtgcccgttc ccgccgaggc gatggatgat gtcgtgggtg atgcggtcgc cctcctgctc
    17281 gcagatcagc acgtcgcgtg ccaggtcggc gcgttcggga aagtccgcca gcatgtcgcg
    17341 caggagcgcg ccggtgcgct ccacgttgcg gcccgactcc tcgagcaggg acagcaggac
    17401 ggggtcagcc ttgcgaacgc gccggaacag gctcacgacg gcatgggcag ttctgcgtct
    17461 gcctctgcgg cgacctcgtc ggcgggggcc tcgggtggga gggtcccttc agccgggacc
    17521 ggctcgaagc ggtagccgat gccgaagtgc gtgtggatgt aggtccagtc cggcgagaag
    17581 cgctcgatct tctgccgcag cttgcgaacg aagacgtcca ccgaccggtc gccccgcacc
    17641 atcgtgtagc cccacacgtg gcggtagacc tcctcgcgct cgagcacgcg cccctcggac
    17701 ccggcgagca gatgcagcag ctcgaactcg cgccgggtga ggtcgatgct ctggcccccc
    17761 acgaacgtct ggaactggtc gggccggatc tcgagctctc cggcgtggac cggccctgcg
    17821 tcctcggacg aatcagcgcg cttgcgccgc cgcacgaccg actccacgcg agcgacgacc
    17881 tcctgcgggt gggtgggctt ggtgacccag tcgtcggcgc cgagccgaag gccgcgcacg
    17941 cgctgggcga ccgtcgactg gcccgtgcag acgatcaccc cgagagccgg gagctcgtca
    18001 cacagccgct cgagaaactc ccacgccgac gatccgagca gcgcgaggtc gacgaccacc
    18061 gcgtgcactc gcatcgacac gagctcgtcg ggccgcaccg gccccgagag gatccggtgc
    18121 tgccagccca tctcctcgac gcgcttcgcg agcacccgga cgaagccgct gtcggagtcg
    18181 acgattgcga gcctgatcgg acctggctgg acgcgcacag taggagcgag ggggctcgtg
    18241 gcaagtgtca atggctgccc catccgcagt tgcaagttta ggtagccggc gagcggcgcg
    18301 tggcgctttc acaggttctt tacacccagg tcacggctcg gtaacggcga tctgcggagg
    18361 atgcgagaac gtgatcaccc atggagagtg cgatccaggg agaggttctt cacgcgggag
    18421 cgctcgaggt tcgaccggcc gaatactccg cggtcgtcgc gggccgccag ctctcgctca
    18481 ccgtgcgtga gcttcagctc cttaccgcgc tcactcgccg ggcggagcgg atcgtgagcc
    18541 gcgaagagct gtacgaggag gtctggggac gccagctcga caagtgcgat cgctcggtcg
    18601 acgtgtacgt gtcgaagctg cgccagaagc tcgagctcgc tctcccgggc agcaactaca
    18661 tacatacgca tttcgggttc ggctaccgat tgtctgcggg cggcagttcc ccctattcgg
    18721 ggtcttcaca tctttttcac accgacgcca caagtcggta acagactgcc gcccagtgcg
    18781 tggcacgctc gcgccgccac gcaaccgaac gaaggagctt cagtgaagtc acgccagtgg
    18841 ctggccatgg ctgccgcctc cgtgcttgcc atcggagtgg cagcgtgcgg cagcagcaac
    18901 aacgacaacg cgggttcgac gggtggaggc ggtgggaagt ccagcggcac cgccgacggc
    18961 gccggctcga cgttcgcagc gcccatctac cagcaatggg gcagcacgct caagggcgac
    19021 ggcgcgacca tcaactacca gccggtcggc tccggcgcgg gcatcgcaca gctcaccgcg
    19081 ggcacggtcg ccttcgcggg cagcgacccg ccgatgaagc ccgaggaaat ctcggccgcc
    19141 aagaagaagg gcgaccccgt ccacatcccg gtcgcgttcg gggcgatcac ggtctcgtac
    19201 aacctcagcg gggtgaagtc ggggctcaag ctcgacgggg cgaccatcgc cgacatcttc
    19261 ctcggcaagg tcaagaagtg gaacgacccg gagatcgcca agctcaaccc gggcacgagc
    19321 ctgccgtcat cgaacatcac ggtgatccac cgctcagatg agtcggggac gaccaagggc
    19381 ttcacggact tcctgcagtc ctacagctcg gagtggaaga gcaaggtcgg ctcggacaag
    19441 accgtgaagt ggcccaccgg aacgggtgcc aagggcaacg acggtgtagc cgccgcgatc
    19501 aagcagaccg ccggctcgat cggctacgtc gagcaggcgt acgccctgca gaacaacttc
    19561 acgaccgctg acgtgaagaa ccagtcgggc aagttcgtgg caccgtcgct cgagtcgacg
    19621 accgcggcag gcgagggcgt gaaggttccg tcgaacctgc agttcacgat catcaactca
    19681 ccgaacccgg ccgcctaccc gatcgcctcg cagacgttcc tgatcgtcta caaggacgac
    19741 tgcaaggcgg gtgtgctgaa gaaggccgac gccccggcgc tcggcacctt cctcaactac
    19801 gccgtgggcc agggccagtc cgtgctcgga cagcttcagt acgcgaagct gcccagcgcg
    19861 atccagacca aggcccagga cgcggtgaag ggtctgcagt gcaacggcag cccgctggga
    19921 ggctctgcgt aaccggatgg aagctcgctc actagagctc cgcggcggcc gctcccttct
    19981 cgagcggtcg ccgctttccg gtattgggga caagatcctt cgctgggtcc tgaccgcgct
    20041 cgcggccggg atcctcgccc tgatcgcgtt cttcttcatc cgcctctatg tggaggcgaa
    20101 gccggcattc gaccgcttcg gcatcttcgg cttcaccttc gacaacaact gggacgtgtc
    20161 gaggcagatc ttcggcgcgc tgccgctggt cgtgggcacg ctcatcacct cggcagtcgc
    20221 gctcttgatc ggcgtcccgg tagctgtcgg cacggctctc tacgtaaccg agttcgctcc
    20281 ccgcccgatt cgggccgttc tctcgttcac gatcgagctg ctcgccgctt tcccctccgt
    20341 cgtgctcggt ctctggggcg tgtacgttct gattccgaag ctgaagccgg cggagcagtg
    20401 gttctccgac acgttcagct tcctgccgtt cgtcggcggc ccggttgccg gtcccaacta
    20461 cttcatcggt ggcctcctgc tcgcgatcat gctgatcccg atcgtcacgg ccatcagccg
    20521 cgaggtgatc agcaccgtgc ccgcggagca caaggaggcc gctctcgcgc ttggtgcgac
    20581 gcggtgggag atgatcaaga tggcggtgct cccctactcg cgcgcgggta tcacgggcgc
    20641 ggcgatgctc gggctcggcc gtgcgatcgg cgagacgatc gcggtcaccc tcgtgatcgg
    20701 caacgcgccg cagatcggca agcagctctt cgaccagggc tacacgctgg cggcggtgat
    20761 cgccaacgag ttcggcgagg cggcgagcga cccggtccac cgggcggcgc tcatcgcggc
    20821 cggcctcgtg ctgttcgtgc tcacgctcgc ggtcaacgcg atcgcgcggc tgctcgtgaa
    20881 ccgcggcgtg cacggccgtc gcgccaccaa ggaggccgcg cccgcatgac cgcaatgcca
    20941 gtcgtctccg cccgccgacg gcgctccgac ctggtcgcgc gcggccttct cgcgaccgcg
    21001 gcgatcgtgg cgctgatccc gctcgtgctc gtcctcttct acctcgtcaa gaagggtgcg
    21061 ggggcgctga gcggcacctt cttctcgagc gatccgactg gcgccttcct cggcaatcag
    21121 ggcggcgtga agagcgcgat cctcgggacg atcgagctcg ttgccctcgc gaccgcgatc
    21181 gccgtgccac tcggcgtcgg cgtcgcgctc tacctcaccg agtacggccg cgacaagccg
    21241 ttcgcaaaag tggtgcgcta cttcgtggac gtgatgacgg gtgtgccgtc gatcgtcttc
    21301 ggcctcttca tctacatcac gctcgtgctc accggagtga gcggcacggg cttcgcgggc
    21361 tggaaggggt ccgtggctct cgcgctgctg atgctcccgg tcgtcacgcg cgcgtccgag
    21421 gtggtgctgc tgctcgtgcc cgagtcgctg cgcgaggcgg cgctcgccct cggtgccccg
    21481 cgctggcggg tcgtggcacg tgtggtgctg cccaccgccc tgcccggcat cgtgaccggt
    21541 acgctgctcg cggtcgcgcg cgccgccgga gagaccgcgc cgctcctgtt cacgtcggcg
    21601 atcgtgaacg gcaccaactt caacctgaac gagcggacga actcgctgcc gatccagatc
    21661 ttcaatgaca tcacgcagcc caacaaccag ctcgtcgagc gcgcctgggg cgcggcgctc
    21721 acgctcgtgt tgatgattct gctgttgacc ctagttgcga gactagtctc gcgaaggagc
    21781 cgactgacat gacgtacgaa cgcgccaagg acgccgagca ggaggctggc accgtgccca
    21841 caccatttcc gcgctcgcag cagccgcgca ccgagtcgtc ggctacgagg acgagcgcgt
    21901 acggggcggc gcgcccgtcc gggacgctcg cacgcaatgt gagcgtccgg ggcctgaacg
    21961 cctggtacgg cgacaaccac gccgtcaaga acctgaactt cgagatcgag ccgaacaagg
    22021 cgacagcgct catcgggcca tccggatcgg gcaagtcgac cgtgattcgc tgcatcaacc
    22081 gcatgcacga ggagatcccg ggcgcacgcg cggacggcga ggtgctgctc gacgaggtga
    22141 acgtgtacga cccgaagatc gacgtcgttg ccattcgccg gcttgtgggg atggtcttcc
    22201 agaagccgaa cccgttcccg acgatgtcga tcttcgacaa cgtcgcggca ggcctgcgcc
    22261 tcaccggcac gaagggagtg ggactgcgcg agcgcgtcga gaagtcgctc cgcgcggcgg
    22321 gtctctggga agaggtgaag gatcggctca aggagcccgg catcagcctc tcgggcgggc
    22381 aacagcagcg gctctgcatc gcgcgcacga tcgccatcga gccagaagtg atcctcatgg
    22441 acgagcccgc atcggctctc gacccgctct cgaccctgcg aatcgaggag ctcgtggacg
    22501 agctcaaggc gcgctacacg atcgtgatcg tgacccacaa catgcagcag gccgcgcgcg
    22561 tggccgactc caccgcattc ctcctggatg gggagctgat cgagcacgcg cccaccgaga
    22621 agatgttcac gaaccccgac gacgagcgca cggagcgcta cgtcaccggg aagttcggtt
    22681 gatgcaggag acccgggtcc acttccagga ggagctggct cagctcgagt cgcaggcgct
    22741 cggcggcctc gacctcgtcg tggaggcgct gaaccgggtg atggaggcgc tcgagcacca
    22801 ggacgtggag ctcgccacga tggtgatcgc cgacgacgac cggatcgacg ggcgctacct
    22861 cgaagtgcac cagggcatcc tgtcgctgct cgcgcgccaa gcgccggtcg cgactgacct
    22921 gcgcctcgtc gccgcgctgc tcgacgtggt gaagcacatg gagcgcatgg gcgatcagtg
    22981 cgtgaacatc gccaagatgc tgccggtcgc cggcaacgag ccgcccgttg aggaggagat
    23041 cctcaagcgc ctcctcaaga tgggccgtca agccgcgttc caagtcgcgc aggcgaagca
    23101 ggcgttcgcg tcgcgcgacg tcgcgctcgc ggaagacctg gtgatacagg acgacgtgat
    23161 cgaccgcctg aaccgcgaga tcttccgaga agtcctcgac tgcagcggcg atgccgacac
    23221 acgcgagtgg tcgctgctga tgatgctcgt ggcgcgcagc atcgagcgca tcggcgacaa
    23281 cgccgtcgac atcggcgagc agacagcgtt cgtggtcacc gggctgttcc gggagttcca
    23341 ggacgcgtcg catccggatc tgaactcgga gtgatccgcg cgccgcgggc gcgcgaataa
    23401 gtcggccaga taccccgtct ggccccaccc atcgacgggg ccgccctcaa gaccgatcaa
    23461 aggagcgagg atgcgacggc tcttcctgct ggtcgccgtg ctggcggccg ctgccatcgc
    23521 ggtaccgctg agcctcggct cgagccaccg cgaggcacca ctcacggcgc tcgaccccac
    23581 cggggacgac accgacgtgt acgcgttcac cgccaacgac gcgcccggcg cgatcacggt
    23641 ggtgagcaac tggatcccgt tcgaggatcc cgcgggcggc ccgaacttct accggttcga
    23701 cgaccgcgcg cactactaca tcaacatcga caacacgggc gacggcgtgg ccgacgtccg
    23761 ttaccagttc aagttcaaga cgaagacctt gaacaagaac tcgttcctct acgccttccc
    23821 gggcgtcagc tccattcgcg actcgaagct gaacgtgcag cagtcgtaca cggtcacgcg
    23881 cgagaagtac aagaacggca agctgaagtc cgcgaaggtc gtcgcggaca atctgccggt
    23941 cgcgcccaac aacgtcgggc ccaagacgat cccgaactac gacgccgtcg ccaaccaggc
    24001 gatccagagc gtcaagggcg gcggcaaggt gttcgccggc cagcgtgacg atccgttctt
    24061 cgtggatctc ggcagcacgt tcgacgcgat caacctgcgc atgggcacgg gcaacgccgg
    24121 cggcggcaag gacgacctgg ccggatacgg cgtgcactcg atcgtcctgc aggtgccgga
    24181 gtcgaaggtc acccgcgacg gcaagcccgt ctcgggccct gcggcgagca acgccgtcgt
    24241 gggcgtctgg tcgtccactg agcgcccgcg cctgcaggtg aacggctcgg cgaatgccgc
    24301 gaagaagaag cggcacagcc gccgctcgca ggacgagaac ttcgtccagg tgagccggct
    24361 cggcaacccg ctgatcaacg aggtcgtgat cccgctcggg cagaaggacc ggttcaaccg
    24421 cacgcagccg aaggacgatg ccaagaactt cggccagtac gcgttgaacc cggagctggc
    24481 caagctgatg aaccagctct tccacgtgaa cgcgccggag accaaccgca cggacatcgt
    24541 gcaggcgctg ctcaccggca tcccgggcaa gacgatgatc tcgaaggacg ccgtgcccgc
    24601 cgacacgctc aagctgaacc ttggcgtgcc gcccacgtcg acacccaacc ggttcggcgt
    24661 gctcgcgggc gacctcgccg gcttccctga cggccgccgc ctcgccgacg acgtcacgga
    24721 catcgagctg cgcgtgatcg ccggcttcct gaaggacaac aaggtgcccc tcggggacgg
    24781 tgtggaccag aacgacaagc cgttcctgtc cacgttcccg tatgtcgccg cgccgacctc
    24841 gggcttcgac tccacgctga agcgcctgga gccgccgcac gagccgacgc cggcccagcc
    24901 gtaaccaccg gatccggggg accgcgccac agtgcggtcc cccgctccgc tccgttctga
    24961 gatcccaatg acgctcgtac gacacaagct cacaagaacc atcgccgccg cggcccttgc
    25021 gttcgccgcg gcgctcgccg tgttcatgct gatgaaccac tcctcccatc cggcgcgctc
    25081 gagcgcagac tcctcagcga gtcctgccag cctcgtgggc gcgagcaccg acacacggat
    25141 ccgcagcttc caggcgctcg tgcgcgcgca cccgcgtgac tcgcgcggct acagcttcct
    25201 tggcgacgcg tacctgcaga aggtgcgcga aacgggggac gcgagcttct acacgcgcgc
    25261 cgatggggtg tacggacgtg cgctcgagtt gaacccgcgc gacgcggccg ccaccaccgg
    25321 catgggcacg ctcgcgctgg cgcgccacga cttccgcgcc ggcctccgct acgggcttcg
    25381 cgcgcatgcg ctcgcaccgg ccgtcgtgaa gccgctcggc gtgatcgtcg acgcctacgt
    25441 ggagctcggg cgctaccacc aagcggccga cgcgctgcag aggatgatcg acctgaagcc
    25501 gaacctcgcg tcctacgcgc gcgtctccta cttccgcgag ct
//
LOCUS       DASVXS010000005         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_566757, whole genome shotgun sequence.
ACCESSION   DASVXS010000005 DASVXS010000000
VERSION     DASVXS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566757"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..947)
                     /locus_tag="VF752_00205"
     CDS             complement(144..947)
                     /locus_tag="VF752_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8853995.1"
                     /translation="MPDARGAASFHVPAEAYDRHVGRYGPALAAELMKAAEIHPDQRA
                     LDVGCGPGALTRALADALGADAVAAVDPSPPFVDACRHRVPGADVRLGAAEQLPFDDR
                     RFDAVLSQLVVNFMADPDLGVSEMCRVAKPGAVVAACTWDYAGGMTLLRRFWDAALLV
                     DREGASARDEAAVMRYGTPPELEVLWRDARLAEVTSGELNVSARYESFDDLWDPIESG
                     VGPAGAYAVALEPRRRAALRSELHRLLGSPDGPFTLPARAWYVTGRVAA"
     gene            971..1393
                     /gene="dtd"
                     /locus_tag="VF752_00210"
     CDS             971..1393
                     /gene="dtd"
                     /locus_tag="VF752_00210"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX8853996.1"
                     /translation="MRAVVQRVSFARVMLGPQIVTEIRAGLLVLLGVHSSDGPEQADR
                     MAAKLEALRIFQDDEGKMNLSVRDVDGEILCVPNFTLYGDTRKGNRPAFVEAAPPEEA
                     ERLYEAVRSSLGALGGRFGEHMSVELANDGPVTLVIET"
     gene            complement(1632..2567)
                     /locus_tag="VF752_00215"
     CDS             complement(1632..2567)
                     /locus_tag="VF752_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8853997.1"
                     /translation="MRLEGIHHVTCITGDGPRNVEFYARVMGLRLSKKTVNQDDPTAY
                     HLFFTDEQGSHGSDLTFFEYPGSTPGRAGAGMIHTIVYRVGSEDSLAFWEERLAGEGV
                     PSERSPGRLRFQDPEGLGLELVVVETTDEPLIAFAADVPDEHALQGFDAVRAYVANPT
                     STERLLRDFMGFQAVAPHDWEARGESRGGHIALEAIQQRGTPGAGTVHHVAWASPDEE
                     HAEWRKQVESGGMHATDIIDRFYFRSIYFREPNGILFEIATEGPGFTADEPLESLGEK
                     LSLPPAYEQLRPQLEPILTPLPDTRKWRRAGVPSS"
     gene            2765..3139
                     /gene="rimP"
                     /locus_tag="VF752_00220"
     CDS             2765..3139
                     /gene="rimP"
                     /locus_tag="VF752_00220"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX8853998.1"
                     /translation="MEPDVEVLLAEQVSAGKVRLVIDRAGGVDLATCERVTHHLRELL
                     NDYALEVSSPGPERPLTKPEHFRRFVGRRAHVKTSVEHGGSKNFTGELVGATDDEVTV
                     AADAGVVSIPYKDINRSNLLEG"
     gene            3144..4607
                     /gene="nusA"
                     /locus_tag="VF752_00225"
     CDS             3144..4607
                     /gene="nusA"
                     /locus_tag="VF752_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX8853999.1"
                     /translation="MSREILEAVTVLEREKGISSERLMSALEDALLSAYKKQPGAARY
                     ARVDMDRESGDFTVYELLVPPELEEQLLAEVEAEAEEAAVDPETGEMREPTEPELDPE
                     RLAEYSDQIDERDVTPHDFGRIAAQTAKQVILQRIREAERDMMFEEYQDRVGELITGI
                     VQQSDSRYTLVQLRERVEALLPKSEQVDNERYDHGQRIKAVITDVSAQAKGPSIIVSR
                     RSPELIRKLFELEVPEIADGLVDITNVAREPGYRSKIAVVSHANGVDPVGACVGPRGS
                     RVRMVVSELRGEKIDIIPYNDEPARFVAKALSPARVREVLVDDDNKQATVIVPDDQLS
                     LAIGKEGQNARLAARLTGWRVDIRSETEFAQEEAEMGYEEEEVAGRCAAILSNGKRCP
                     NAALPGSRYCGLPAHQALADVEGDEVAAVIGGPTAEAEEEAGVDPGAELEGAETIEEE
                     VVEATAAEEEAAQPEAQAEAELEVEPAEQERAEETAS"
BASE COUNT          820 a         1687 c         1802 g          718 t
ORIGIN      
        1 gtacgccaac gtcgagcggg tagccgccgg cgtgctcggc gtcgacggag atgccccact
       61 cggtgtgggc cagcctccgg gtctcgccgg gcagggcctg gaggtagctg tcgacgacgt
      121 cggcggcgga catggccctg actctaggcg gcgaccctgc cggtcacata ccaggcgcgc
      181 gccgggaggg tgaacgggcc gtccggggag ccaagcagcc ggtggagctc tgagcggagc
      241 gctgcgcgcc ggcgaggctc gagcgccaca gcgtacgcgc cggccgggcc gacgccgctc
      301 tcgatcgggt cccacaggtc gtcgaagctc tcgtaacgcg cgctcacgtt gagctcgccg
      361 gatgtgacct ccgcgagacg tgcgtccctc cagaggacct cgagctccgg cggtgtgccg
      421 tagcgcatca cggctgcctc gtctcgagcg gacgcaccct cacgatccac gaggagcgcc
      481 gcgtcccaga agcggcgcag aagcgtcatg ccccccgcgt aatcccacgt gcaggccgcg
      541 accacggcac ccggcttcgc gacccggcac atctcgctga cccctaggtc cggatcggcc
      601 atgaagttca cgacgagctg cgagagcacc gcatcgaacc tgcggtcatc gaacgggagc
      661 tgctccgcgg cgccgagccg cacgtctgcg cccggcaccc gatgccgaca tgcatccacg
      721 aacggcggcg aggggtccac ggccgccacc gcatcagcac cgagcgcgtc tgccagcgcg
      781 cgcgtgagag ctcccggccc gcagcccacg tcgagcgcgc gctggtccgg gtggatctcg
      841 gcggccttca tcagttcggc ggcaagggcc gggccgtagc gtcctacgtg ccggtcgtac
      901 gcctccgcgg gcacgtggaa gctggctgcg cctcgggcat cgggcattcg cggagagtac
      961 ggttccaccg atgcgggcgg tggtccagcg cgtgagcttc gcgcgggtga tgctcggccc
     1021 ccagatcgtg accgagatcc gcgccgggct cctggtcctg ctcggcgtgc actcgagcga
     1081 tgggcctgag caggcggacc ggatggcggc gaagctcgag gcgctgcgga tcttccagga
     1141 cgacgagggc aagatgaacc tctcggtgcg cgacgtggac ggcgagatcc tgtgcgtgcc
     1201 gaacttcacg ctgtacggcg acacgcggaa gggcaatcgc ccggcgtttg tggaggcggc
     1261 gccgccggag gaggccgagc ggctctacga ggcggtgcgt tcttcgctcg gggcgctcgg
     1321 cgggcggttt ggggagcaca tgagcgtgga gctggcgaac gacgggccgg ttacgttggt
     1381 catcgagacg tagctggttg gctctggtcc cgcgcgctcg ctcttgcgtt ggttggggca
     1441 gtgaggggga ggggcagggg tgcataagtt ccatctcgac cccaatcgcc gggtgcgatg
     1501 gactttgtgg tccgtatcgg tctatttctt ccatcgcgaa ctggacgagc gctcgcgatg
     1561 gaagctttga acccctcccg ggcccacccc taagtgctca accgaaccgc gccgcggcca
     1621 aggccgctca gttacgacga aggcacgccc gcccggcgcc acttccgcgt atcaggaagc
     1681 ggcgtcagga tcggctcgag ctgcgggcgc agctgctcgt acgccggcgg gagcgacagc
     1741 ttctcgccga gcgactcgag cggctcgtcg gcggtgaacc cgggcccctc ggtcgcgatc
     1801 tcgaacagga tcccgtttgg ctcgcggaag tagatcgagc ggaagtagaa gcggtcgatg
     1861 atgtcggtcg cgtgcatgcc gccgctctcc acctgcttgc gccactcggc gtgctcctcg
     1921 tcgggcgacg cccaggccac gtggtgcacg gtgcccgcgc ccggcgtgcc gcgctgctgt
     1981 atggcctcga gggcgatgtg gccccctcgt gactcgccgc gcgcctccca gtcgtgaggc
     2041 gccaccgcct ggaagcccat gaagtcccgc agcagacgct cggtggacgt cgggttggcg
     2101 acgtaggcgc gcaccgcgtc gaagccctgc agcgcgtgct cgtcagggac gtcggcggcg
     2161 aacgcgatca gtggctcatc ggtggtctcg accacgacca gctccaggcc cagtccctca
     2221 ggatcctgga agcgaagccg ccccggcgag cgctcgctcg gcacgccctc gccggccagg
     2281 cgctcctccc agaacgccag cgaatcctcg gagccgaccc gatacacgat cgtgtggatc
     2341 attcccgcgc cggcgcgccc tggcgtcgat ccggggtact cgaagaaggt caggtccgag
     2401 ccatgcgagc cctgctcgtc ggtgaagaag aggtgatacg cggtcggatc gtcctggttc
     2461 accgtcttct tcgagagcct cagccccatc acgcgtgcgt agaactcgac gttgcgaggg
     2521 ccgtcgccgg tgatgcaggt cacgtgatgg atgccttcga gccgcatggg cactcccact
     2581 cctaaagacg cctggcctat acgtcaggga gggtacagcg cgtctatact cgaacccgct
     2641 gaaactttca tctgcggccg cagagccgca cctggaggtg ggcttcgcaa gcccaccttt
     2701 tttattcgat tggacgagga cgatgcgacc ggaacagacc gagatagcgc agaggctgcg
     2761 ggccgtggag cccgacgtcg aggtgctgct cgcggagcag gtctccgccg ggaaggtgcg
     2821 cctcgtgatc gaccgtgccg ggggcgtgga tctcgcgacc tgcgagcgcg tcacccacca
     2881 tctgcgcgag ctgctgaacg actacgcgct cgaggtgtcg tctccgggcc cggagcgtcc
     2941 gctcaccaag ccggagcact tccgccgttt cgtcggccgc cgcgcgcacg tgaagacgag
     3001 cgtcgagcac ggcggcagca agaacttcac gggcgagctc gtcggcgcga ccgacgacga
     3061 ggtcacggtc gcggccgatg ccggcgtggt ctcgatcccg tacaaggaca tcaacagaag
     3121 caatctgctg gagggctgat cgcatgagta gagagattct cgaagcagtc acagtcctcg
     3181 agcgtgagaa gggcatctcc tcggagcgcc tcatgagcgc cctcgaggac gccctcctgt
     3241 cggcatacaa gaagcagccg ggcgctgcgc gctacgcgcg cgtcgacatg gaccgcgagt
     3301 cgggcgactt cacggtctac gagctgctcg tcccgccgga gctcgaggag cagctgctcg
     3361 ccgaggtcga ggccgaggcc gaggaagctg ccgtcgaccc cgagaccggt gagatgcgcg
     3421 aaccgaccga gccggagctc gaccccgagc ggctcgcgga atacagcgac caaatcgacg
     3481 agcgcgacgt cacgccgcac gacttcggcc gcatcgccgc tcaaaccgcc aagcaggtga
     3541 tcctccagcg catccgcgag gcggagcggg acatgatgtt cgaggagtac caggaccgcg
     3601 tgggcgagct gatcacgggg atcgtgcagc agtccgattc gcgctacacg ctcgtgcagc
     3661 tgcgcgagcg cgttgaggcg ctgcttccga agtccgagca ggtcgacaac gagcgctacg
     3721 accacggcca gcgcatcaag gccgtgatca ccgacgtctc ggcgcaggcg aagggcccga
     3781 gcatcatcgt gtcgcgccgc tcgccggagc tcatccgcaa gctgttcgag ctcgaggtgc
     3841 ccgagatcgc cgacggcctc gtggacatca cgaacgtcgc gcgcgagccg ggctaccgct
     3901 cgaagatcgc agtcgtgtcg cacgccaacg gcgtggaccc ggtcggcgca tgtgtgggcc
     3961 cgcgcggctc gcgcgtgcgc atggtcgtgt ctgagctgcg cggcgagaag atcgacatca
     4021 tcccgtacaa cgacgagccc gcccgcttcg tcgccaaggc gctctcgccc gcgcgcgtgc
     4081 gcgaggtgct cgtcgacgac gacaacaagc aggcgaccgt gatcgtgccg gatgaccagc
     4141 tgtcgctcgc gatcggcaag gagggccaga acgcccgcct cgccgcgcgg ctcaccggct
     4201 ggcgcgtcga cattcgctcg gagaccgagt tcgcccagga agaggctgag atgggctacg
     4261 aggaggaaga ggtcgccggc cgctgcgccg cgatcctctc gaacggcaag cgctgcccga
     4321 acgccgcgct gccgggctcg cgctactgcg gcctcccggc gcaccaggcg ctcgccgatg
     4381 tggagggcga cgaggtcgcc gcggtgatcg gcggtcccac ggctgaggcc gaggaggagg
     4441 ccggcgtgga cccgggcgcc gagctggagg gtgccgagac gatcgaggaa gaggttgtcg
     4501 aggcgacggc ggccgaggag gaggccgccc agcccgaggc gcaggcggag gccgagctcg
     4561 aggtagagcc cgcggaacag gaacgggcgg aggaaaccgc ctcatagggc acgtcccgca
     4621 gaggcgctgc gtgggctgcg gccggtcgcg cccgaaggcc gagctgctgc gcttcgtggc
     4681 gggcccggag gggctcgagc gggacttcga cgggaggcgc ccagggcggg gcgcctacct
     4741 ctgcagggaa gccgacgccg accgacccgc agccgaatgt gcgcgcctgg cggttgagcg
     4801 gaagggcttc gaacgggcgc tgcggcggcc tgtgcgcata gagggacagg tcatagactt
     4861 cgactccacc aggagctaga tggcaaagaa gagagttcac gagatcgcca aggagcgcgg
     4921 gatcccctcg aaggaggtga tcgccgtgct gcagcgcgcc ggcctcgacg tgaaggccgc
     4981 cgcctcgagt gtcgacgagc gcgacatctc tcgcgccttc tcgaacg
//
LOCUS       DASVXS010000006        17993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1062227, whole genome shotgun sequence.
ACCESSION   DASVXS010000006 DASVXS010000000
VERSION     DASVXS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062227"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VF752_00230"
     CDS             <1..595
                     /locus_tag="VF752_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8854000.1"
                     /translation="RRDAPPQRPRPEALEESDPMSPSAAVQELADAFPADGIVVLESP
                     SATLALRNRLRLDKPGSYYFGAGGGLGFGVSAALGVQLAQPERPVVCVVGEGSAQYAI
                     QGFWTASAYSVPVTFLVLRNEEYAILKWFGMLENVSGAPGLDLPKLDVASVAEGYGVS
                     ARRVTETGELGDALREAIAAEEPRVVEVRVTPGMALT"
     gene            complement(597..1607)
                     /locus_tag="VF752_00235"
     CDS             complement(597..1607)
                     /locus_tag="VF752_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX8854001.1"
                     /translation="MRRLLPLALLVCLVAGCGSTKVEREPPLSVTGAQSPSVPNPGTP
                     GARGVRIIVVTHGQASSPFWAIVRNGVEAAARQMDVLVSYRAPDVYSLPLMKKLIEQA
                     VASRPDGLVVSIPEPGVAPAIRRAVKDGIPVVSINSGSDIAPKLGVLAHVGQPEDRAG
                     LQAGERLARAGVRRALCVNQQVGNVGLDARCRGLAKAMRAVHGSAEVIAVNDQSSETP
                     KRIANAITVGRIDGVLTLNSTTGTEAVSAVRSLHMEGSVKIGTFDLGPDVLTAVKDGQ
                     LDFAVDQQAYLQGYLPVIYLAQRARYGLFPSQGDVVATGPNFVTKQNAGKAIELSRRS
                     IR"
     gene            complement(1604..3172)
                     /locus_tag="VF752_00240"
     CDS             complement(1604..3172)
                     /locus_tag="VF752_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX8854002.1"
                     /translation="MIARLELGRRVEHASQAIAYLLANVPIAALGLLAVLALILGAAL
                     SVIGIGLPIVLGAAAACRWLIRLDRRAANSLLATQIPPLPGGPRTSGSPWRRSLDVLS
                     DRVLWRAVAVLATKPLLIGVLLAAALAPLALVAELLNLGVQGVTGLGGMDFVGPLSFG
                     FGTGVVLLVLALPAAVVAIATLEALRTLLCTITRALVAPRVMAAGPVREMLAESLGDR
                     SISVAYWLPDRQVFVDEVGRRVTLPDPGSGRAWTAVERNGRPVAAIVHDAALDTSREL
                     VQAAAAASSLAIDNERLKADLRARLEELRVSRLRIVEATDAARRRIERDLHDGAQQQL
                     VSLRLELRTLKTRTKNLDSETAKLVDRLAVRLEEAISELRELARGIHPAVLTERGLGP
                     AVETLAERTPVAVEREVAVEERLPARLESAAYFTVAEALTNVVKYAKASKARVEIRRA
                     GAELVVVVTDDGIGGADMSKGTGLSGLQDRLAAVDATLAVDSPPNGGTRVEARLPLDR
                     GLESAERPVAEVRV"
     gene            complement(3169..3903)
                     /locus_tag="VF752_00245"
     CDS             complement(3169..3903)
                     /locus_tag="VF752_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX8854003.1"
                     /translation="MATEARTRRLSPWARVVTVSAVIVVGSLVALGAWALASREKRVV
                     SYDVRGPVSGVALDLGDGDVKIVGGGRRSGIAVRRSERFTFGHDADTRRSVDGSTFRV
                     RSRCPETVLHSCSVSYTLVVPDNVPVSVRTTSGDVGFDGYRGSAQVDTTSGDVRMTGF
                     CGFSLQARAESGAVDVATSCPPPQLSLRTTSGDIHVVVPPGRYSIDAESSSGSHSFRG
                     VSSGADAPLSIQALSNSGDVLVEGRR"
     gene            4125..4550
                     /locus_tag="VF752_00250"
     CDS             4125..4550
                     /locus_tag="VF752_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8854004.1"
                     /translation="MIQTIAVGTDGSETAGAAVDAAVEIAERFGAKVVLLSAFENGES
                     AYPVEGATLELQWAKNPASLVREILQRTEDEVRRRGLECATLVDEGHPGDVIVRLAAE
                     CNADMLVVGNKGMQRRVLGSVPNTVAHKAPCSVYVVKTT"
     gene            complement(4537..5313)
                     /locus_tag="VF752_00255"
     CDS             complement(4537..5313)
                     /locus_tag="VF752_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8854005.1"
                     /translation="MGGNGKPVLWHIPFSHYSEKARWALAFKGIAHDRHVAVAGTHPA
                     VAFALTRGQCGTFPVLQIDGRNIGDSTAIIAALEEAQPDPPLYPADPDERRRALELED
                     WFDEHLGPQARLLGWHEVVMDPESLESLSLKMAPALRRFPAVAAWATRSFVNARFKVS
                     SREAAERARADVLTAFDRLESELGDSDYLVGDSFSVADLTAAALFYPLVLPPEAPRQI
                     DNPPERYERFREPLKERRGYRWVEEMYRRHRRPAPVATSS"
     gene            5376..5708
                     /locus_tag="VF752_00260"
     CDS             5376..5708
                     /locus_tag="VF752_00260"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8854006.1"
                     /translation="MVQYSPVDQTLAALADPTRRAVLERLGGGSASISELAEPFGMSL
                     TGMKKHVRVLENAELVSTEKVGRTRHCTLGPRRLEDLEQWIADYRRMLDERLERFGEL
                     LERQKGVS"
     gene            5705..6202
                     /locus_tag="VF752_00265"
     CDS             5705..6202
                     /locus_tag="VF752_00265"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8854007.1"
                     /translation="MTSQEQQAQAATITTPSDREIRVERVFNAPRDQVFSVYTNPDLI
                     PEWWGPRGMTTVVDEMDVRRGGRWRFVMREPDGSESGFHGTYREVTPPERIVQTFEWE
                     GMPGHVCVETATFEDLGDRTRVTTVSIFHTTEERDGMLASGMEGGLNETYARLDELLE
                     RLAAR"
     gene            complement(6262..6639)
                     /locus_tag="VF752_00270"
     CDS             complement(6262..6639)
                     /locus_tag="VF752_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854008.1"
                     /translation="MTTLITGTDFITIPTRDFDKAVEFYGGVLGLPEGKRWGQMPGAE
                     FETGNLTVAVMQSDAFGLEFRPHSHPVEFHVDDFDAAHAELESRGVSFKGDTIDSGVC
                     FQAFFEDPDGNMLAIHHRYAPKD"
     gene            complement(6686..6862)
                     /locus_tag="VF752_00275"
     CDS             complement(6686..6862)
                     /locus_tag="VF752_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854009.1"
                     /translation="MGSVGLCIVLWIRAKTVDQDERGNAERRALFVGLWPPTLYLIGE
                     SLERQEQHGRRLLW"
     gene            6998..8443
                     /locus_tag="VF752_00280"
     CDS             6998..8443
                     /locus_tag="VF752_00280"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HEX8854010.1"
                     /translation="MPGLPLSRRLATAARWPIGVGLTSWRYMWRTTPFHRGERAGSPA
                     EDLAPPLPENVSREAIQGPEDGIGPLFHRRYGVRVRDPHMPARELMSRLQADPDAAAP
                     GEFATFIKLQGAEGRMRVGDEFVVRMAGPWDGPVRVIETTDTSFRLATLSRHLEAGQI
                     EFSAADTDAGLEFRIESWARSADRLADILYQHLRMAKEIQLHMWASFLERVAEIAGGR
                     VTGGIAIDTRRVEAPVQPAVAHLRRLALNFDPECYEQHVPENGWHVDDLRTRLPPEPP
                     GPPVEGGSWEVARRLMRGYEFADPSIVRASYDPDEPLEGRNMLLELRFHGLRFHGGVR
                     VSQVYDEEVTRDRRTARVWGWAYQTLEGHLEMGQMDWQVWKWLDTGEVEFRIHAYSRR
                     ARDRNAIVRFGFWLFGRREQLAFLHSTLERMRRLTETALEKGGGEPVRKTAEQLTARP
                     ARGTARAHSRLAAYLEDGHEHSTPTEPPNRA"
     gene            complement(8564..8637)
                     /locus_tag="VF752_00285"
     tRNA            complement(8564..8637)
                     /locus_tag="VF752_00285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8601..8603),aa:Pro,seq:ggg)
     gene            complement(8639..8709)
                     /locus_tag="VF752_00290"
     tRNA            complement(8639..8709)
                     /locus_tag="VF752_00290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8675..8677),aa:Gly,seq:tcc)
     gene            8857..9120
                     /locus_tag="VF752_00295"
     CDS             8857..9120
                     /locus_tag="VF752_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854011.1"
                     /translation="MSEEKKPPRSTNASGVPVGSDEHSLTAGPNGPTVLHDAYTVQKM
                     QQVAIGTRRRTPRVVGRYAGIQQRAEVRQIHPKRVLDPVVPVD"
     gene            9130..9441
                     /locus_tag="VF752_00300"
     CDS             9130..9441
                     /locus_tag="VF752_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854012.1"
                     /translation="MLAEAITFSALLVLTAAAAPYLRSRLPLSADNVIRTIMPAPLVL
                     MASGTLAVGDHGEAALAVAYVGVQMTAIACLFVPTRRSRWSRFERQFRAYATKAEHVR
                     Q"
     gene            complement(9483..10019)
                     /locus_tag="VF752_00305"
     CDS             complement(9483..10019)
                     /locus_tag="VF752_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8854013.1"
                     /translation="MRRTSKELERLGRRAAREVDGTVQRVRHLRSEPKPDMDDVTLAR
                     KVETVLFRRLPDVKGHVNINVVDHVVVLHGQAKNPTRIRQIESIVRGIPEVTGIESNL
                     HLPKTPARTRADSPGARKGGTRKTSKPRDTGKRFNAEKTPAEPGDTPAEVAAGGSGRR
                     AAPLGSTGDEETEPDSTA"
     gene            complement(10161..12470)
                     /gene="lon"
                     /locus_tag="VF752_00310"
     CDS             complement(10161..12470)
                     /gene="lon"
                     /locus_tag="VF752_00310"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8854014.1"
                     /translation="MSTTNEGTTLENLILIPLEDTVVFPNMNVTLAIDTGDEDRVLLV
                     PRHENEFGRVGTVAEVVEKVRLPGGAQAVSLFGQHRAIIGAASTGPDGRLRVQVEPHP
                     DDTPVDGRTRNAEREYRAVVEEILELRGDDGRIAAFVRSIVEPGALADTAGYSPDLGF
                     DQKRELLETLDVTERLELALEFQRERLAEQQVRDRIREDVRSGAEKQQREYFLRKQME
                     SIQKELGEDEGSVIDEYRAKIEEAGMPEHVREQADKELGRLERMGEQSPESSTIRSYL
                     DWLIAVPWSKRSDERLDPVHAREVLDADHAGLEDVKQRIVEYIAVKKLREERGIAEDK
                     RSGAILTLIGPPGTGKTSIGESIARATGREFVRMSLGGVRDEAEIRGHRRTYIGALPG
                     RLVRALRDADTMNPVIMLDEVDKVGADWRGDPSAALLEVLDPAQNHAFRDHYLDVEID
                     LSQVLFIATANVADTIPAPLLDRMETIRFDGYTTEEKVAIAKGYLWPRQRERNGLNED
                     EVTVSDDVLRQVVTEYTREAGVRNLERELGRVLRKTATRIASGEATAPVEIGTEQVRD
                     ALGRQRFHQEAAERTAVPGVATGLAVTGVGGDVLFVEATGMRGNDGLVLTGQLGEVMK
                     ESARIALSYVRSHADALGIDPDEFDKREFHVHVPAGATPKDGPSAGITMTTALASLLS
                     GRPVRHTVGMTGEVTLQGRVLPIGGLKQKVLAAHAAGLTDVILPERNRGDLDDVPEDV
                     RDQMTFHPVMTIGEVLELALEPAPAPAVA"
     gene            complement(12802..13920)
                     /locus_tag="VF752_00315"
     CDS             complement(12802..13920)
                     /locus_tag="VF752_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX8854015.1"
                     /translation="MHTQLPAPALVSPYGKRADLIVHQAEPFNAETPPAALAEGPVTA
                     TDAFYVRGHGPVPDLDPSAWHVRVHGLVERTLEVSIGTLREAFQERELTATLQCAGNR
                     RAGLVSIRDIPGEAPWGPGATGTATWTGVALVDVLALAGPLREAAHVGFEGADLCSQA
                     KPSERFGGSIPLDKACRPEVLLAWGMNGEPLPPLHGGPLRVVVPGYIGARSVKWLERI
                     EVRSTPWHGYFQDVAYRLLPEDGRPGPGAGMPLGLVALNADVLSPADGATVTAGPVVV
                     RGYAFAGGERHVARVDVSLDGGASWSQAELLDDLGRWAWRHWRTAVELEPGEHEIVVR
                     AWDSSAATQPEDEAALWNPKGYVNNARPRVRIRATAAP"
     gene            complement(13993..14982)
                     /locus_tag="VF752_00320"
     CDS             complement(13993..14982)
                     /locus_tag="VF752_00320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8854016.1"
                     /translation="MDSHGTERILSSAWGGVWTTLLSCGAAGAYVASTWDGPNRTAIA
                     ALIGGALGFSLLVPFVPLRRLLTTRTREVAFTLSWNLGVIVLIAAGAALDGGVASPLS
                     YLGFVALVFASVSYSSTRLVAAVAAVDMGSFVLVGFLASETAWPHVMLFLGSLTIVSG
                     ASVWQTSIGRRQRRELALASRTDPLTACLNRRGLEERLEAALADARRDELPLSLIQLD
                     FDNFKAVNDTNGHAAGDAMLVEAVKRMRSVVRDTDVLGRMGGDEFAIVAVGAGRRDTE
                     ALAKRLEAAIAPASASVGFALFPVNGETADDLQRAADARMYEVKRARAAAAAA"
     gene            complement(15221..16795)
                     /locus_tag="VF752_00325"
     CDS             complement(15221..16795)
                     /locus_tag="VF752_00325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8854017.1"
                     /translation="MGVGLGRAPAEGYTDAPGGQTREEIGPGRFRGRRSSLVLFKRRP
                     DPAFEIREGDRDVAVLGRDATFKRALVAADLLAALAALVFIAGVGGDTLQPGALALLP
                     LVVLASKTAGLYDRDEHVLGKGTLDEATSLFHVAIAFTLLTWLASPAVFDGRLDRLEV
                     VGVLAALFAALVANRIAARRLGLAVTLPERCLVLGDAVAARELMRRTERSKLVNAIVV
                     GRVPLEGRRPHGIDTSVRPATVPGDPPVLGTLPGLAAVLWAQQIDRVIIAPGSDAGSD
                     RLLDKIRLIKAVGVKVTLVPRLLEVVGSAVEFDDVDGMQLLGVRVSQLSRSSRRLKRL
                     LDVTGSLLGLIVLAPLFALVAVAIKLDSRGSVFFGQRRVGLWAREFPLLKFRTMVEDA
                     DSHKGELAALNEAEDGFFKIADDPRLTRVGKVLRRLSIDELPQLFNVLRGEMSLVGPR
                     PLVPEEDIRVQGWLRRRLDVQPGMTGVWQVLGSSRVPLRDMVRIDYLYLANWSLWLDL
                     KILLRTVPLVLGRRGQ"
     gene            17319..>17993
                     /locus_tag="VF752_00330"
     CDS             17319..>17993
                     /locus_tag="VF752_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX8854018.1"
                     /translation="MGGLAGISQGGGRAWRRIRFTLWALRLRGRLARHGCRLDVSAPH
                     GAPLFERTPVVRLHTEGDGRGSLTLRFGAGVSFGRDVVLEVWARQANVLELGAGTRLE
                     DRVHVALRGGHVHLGSESTVHEGGEIHVEGLFITGRRAYVGSGAVVRCTQRIELGERV
                     TLAERCSISDLGHAQAGSGSNGGSEPVIEPVEIPSDTLVGPNSVLTQGVRLGEGSIVE
                     AGALVYP"
BASE COUNT         2756 a         6341 c         6232 g         2664 t
ORIGIN      
        1 ccgccgcgac gctccgccgc agcgtccgcg tccggaggcg ctcgaggagt cggacccgat
       61 gagcccctcg gccgcggtgc aggagctcgc cgacgccttc cccgccgacg ggatcgtggt
      121 gctggagtcg ccgtccgcga cgctggcgct gcgaaaccgc ctgcggctcg acaagcccgg
      181 gagctactac ttcggcgcgg gcggcgggct cggcttcggg gtctcggccg cgctcggcgt
      241 gcagctcgcg cagccggagc ggccggtggt gtgcgtcgtc ggcgagggct cggcgcagta
      301 cgcgatccag ggcttctgga ccgcatccgc ctacagcgtt ccggtcacct tcctcgtgct
      361 gcgcaacgag gagtacgcga tcctcaagtg gttcggcatg ctcgagaatg tgagcggcgc
      421 gccgggtctc gacctgccga agctcgacgt ggcctcggtg gccgaggggt acggcgtgag
      481 cgccaggcga gtgaccgaga cgggcgagct cggcgacgcg ctgcgcgaag cgatcgccgc
      541 cgaagagccg cgcgtggtcg aggtgcgcgt gacgccgggg atggcgctca cctgagtcag
      601 cggatcgagc gccgcgagag ctcgatcgcc ttgccggcgt tctgcttcgt cacgaagttc
      661 gggccggtcg ccaccacgtc gccctgcgac gggaacaacc cgtaacgcgc acgctgcgcg
      721 aggtagatga ccggcaggta gccctgcagg taggcctgct ggtcgaccgc gaagtcgagc
      781 tggccgtcct tcaccgccgt cagcacgtcc ggcccgaggt cgaaggtgcc gatcttcacc
      841 gagccttcca tgtgcaggct gcggaccgcg ctcaccgcct ccgttccggt ggtcgagttt
      901 agcgtgagca cgccgtcgat ccggccgacg gtgatcgcgt tcgcgattcg cttcggggtc
      961 tcggagctct ggtcgttcac ggcaatgacc tcggcgctgc cgtgcaccgc gcgcatcgcc
     1021 ttcgcgaggc cgcggcagcg cgcgtccagg cccacgttcc ccacctgctg gttcacgcac
     1081 agcgcccggc gcacgccggc gcgcgcgagc cgctcgcccg cctggagccc cgcccggtcc
     1141 tccggctggc caacgtgcgc gagcacgcca agctttggcg cgatgtcgct tccggagttg
     1201 atcgagacga ccggtatgcc gtccttcacc gcccgccgga tcgcgggcgc gacgcccggc
     1261 tcggggatcg acaccacgag accgtcaggg cggctcgcca ccgcctgctc gatcagcttc
     1321 ttcatcagcg ggaggctgta gacgtcgggc gcgcgatagc tcaccagcac gtccatctgc
     1381 cgcgcggctg cctctacccc gtttcgcacg atggcccaga agggactcga tgcctggccg
     1441 tgcgtgacga cgattatccg cacgccgcgc gccccgggcg tgcccgggtt cggcaccgac
     1501 ggggactgcg cgccggtcac gctgagcggc ggctcgcgct cgaccttcgt gctgccgcac
     1561 cctgcgacca agcacacgag cagcgcgagc ggcagcaggc gcctcacacg cggacctccg
     1621 ctacggggcg ctcggccgac tcgagtccgc ggtccagagg gagccgcgcc tcgacgcgcg
     1681 tgccgccatt cggcgggctg tcgacggcga gcgtcgcgtc gaccgccgcc agccggtcct
     1741 gaaggccgct caggccggtg cccttcgaca tgtccgcgcc gccgatgccg tcgtccgtga
     1801 ccacgaccac gagctcggcg cctgcgcggc ggatctcaac gcgcgccttc gaggccttcg
     1861 cgtacttcac cacgttggtg agcgcctcgg ccaccgtgaa gtaggccgcg gactcgagcc
     1921 gcgccggcag ccgctcctcc acggccacct cgcgctcgac ggccacgggc gtgcgctcgg
     1981 cgagcgtctc gacggccggc ccgagcccgc gctcagtgag cacggcggga tggataccgc
     2041 gcgcgagctc gcgcagctcg ctgatcgcct cttcgaggcg aaccgccagg cggtcgacga
     2101 gcttggccgt ctcggagtcg aggttcttcg tccgagtctt cagcgtgcgc agctcgagcc
     2161 gcagcgagac gagctgctgc tgcgcgccgt cgtggaggtc gcgctcgatc cggcgccgcg
     2221 cggcgtcggt ggcctcgacg atacgcagcc gcgacacgcg cagctcctcg agacgcgctc
     2281 ggaggtcggc cttcaggcgt tcgttgtcga tcgcgagcga cgaggccgcc gcggccgcct
     2341 gaaccagctc acggctcgtg tcgagcgcgg cgtcgtgcac gatcgcggcc accggccgcc
     2401 cgttgcgctc gaccgcggtc caggcccggc cggacccggg gtcgggaaga gtcaccctcc
     2461 tccccacctc gtcgacgaac acctgccggt ccggcagcca gtacgcgacg gatatcgagc
     2521 gatcaccgag gctctcggcg agcatctcgc gcaccgggcc ggcggccatc acgcgcgggg
     2581 ccacgagcgc gcgtgtgatg gtgcagagca gtgtgcgaag cgcctcgagc gtcgcgatcg
     2641 ctacgaccgc ggcaggcagc gcgagcacga gcagcaccac gccggttccg aagccgaagc
     2701 tcaaggggcc gacgaagtcc atgccaccca ggcccgtgac tccctgcacg ccgagattca
     2761 ggagctcggc gacgagcgcg agcggagcga gtgccgccgc gagcagcacc ccgatcagca
     2821 gcggcttggt cgcgagcacg gccaccgcac gccagagcac gcggtcggac aggacgtcga
     2881 gcgagcgccg ccacgggctg ccggaggtgc gcgggccccc cggaagaggc gggatctgcg
     2941 tggccagcag gctgttcgcg gcgcgccggt cgaggcggat cagccagcgg caggccgcgg
     3001 cggcgccgag cacgatcggc agcccgatgc cgatcacgct gagcgctgcg ccgaggatga
     3061 gcgcaaggac cgcaagcagc ccgagcgcgg cgatcggcac gttcgcgagc aggtaagcga
     3121 tcgcctggct ggcgtgctcg acgcggcggc cgagctcgag ccgggcgatc atctgcgccc
     3181 ctccaccagg acgtcgcccg agttgctgag cgcctggatc gacagcggcg cgtcagcccc
     3241 actgctgaca cccctgaagc tgtgcgagcc cgagctgctc tcggcatcga tgctgtagcg
     3301 gccgggcggc acgaccacgt ggatgtcacc gctcgtggtg cgcagcgaca gctgcggggg
     3361 cgggcacgaa gtcgcgacat caacggctcc gctctccgcg cgcgcctgca gcgagaagcc
     3421 gcagaagccc gtcatccgga cgtcgccgct ggtggtgtcc acctgggcgg agccgcggta
     3481 gccgtcgaag ccaacgtcgc cgctcgtggt tcgaacactc accggcacgt tgtccggcac
     3541 caccagcgtg taggagacag agcacgagtg cagcaccgtc tccgggcagc gtgaacgcac
     3601 ccggaaggtg ctcccgtcga cgctccttcg tgtatcggcg tcgtgcccga acgtgaagcg
     3661 ctcgctccgc cgcaccgcga tcccggatcg ccggccaccg cccacgatct tcacgtcgcc
     3721 gtcgcccagg tcgagcgcca ccccgctcac cggtccgcgc acgtcatacg agacgacgcg
     3781 cttctcgcgc gatgcgagcg cccaggcacc gagggcgacc aggctgccga ccacgatgac
     3841 cgcggagacc gtgaccaccc gcgcccacgg cgagagccgt cgagtccgag cttcggtggc
     3901 cacgagcgga ctctatcccc gaccgcgggc ctacttgcgg cggagcggcc ggacgagcag
     3961 gctccgcccc gccgtcacga gggtcgtctt ctcaggcgtg gagtcgcgca cagccactcg
     4021 agccaaccct gccacaaaac ggtcgctcgg gccacctttt cggggtgggt tcctacccgt
     4081 tcggacatgg actcgggcac tcccgctcgg agatactcgt tcccatgatc cagacgatcg
     4141 cagtgggcac ggacgggtcg gagacggcgg gcgcggcggt cgacgcggcg gtggagatcg
     4201 cggagcgctt cggcgcaaag gtcgtcctcc tgagcgcgtt cgaaaacgga gagtccgcgt
     4261 accccgtgga gggcgccacg ctcgagctcc agtgggcgaa gaatccagcg tcgctcgtgc
     4321 gggagatcct ccagcggacg gaggacgagg tccggcgtcg cgggctcgaa tgcgccacgc
     4381 tcgtggacga gggccatccc ggcgacgtga tcgtgcgcct ggccgcggag tgcaacgccg
     4441 acatgctcgt ggttggcaac aagggcatgc agcggcgcgt gctcggaagc gttccgaaca
     4501 ccgtcgcgca caaggcgccg tgctcggtct acgtggtcaa gacgacgtag cgaccggcgc
     4561 cggccggcgg tgccgccggt acatctcctc gacccagcgg tagcctcgcc gctccttgag
     4621 cggttcgcgg aagcgctcgt agcgctccgg cggattgtcg atctggcgcg gggcctcggg
     4681 cggcagcacc agcggataga acagagcggc ggccgtgaga tcggcgacgc tgaagctgtc
     4741 gcccacgagg tagtccgaat cgcccagctc cgactcgagg cggtcgaacg ccgtgagtac
     4801 gtccgccctc gcccgctcgg ccgcctcgcg gctgctcacc ttgaaacgcg cgttcacgaa
     4861 cgagcgtgtc gcccacgccg ccacggccgg gaagcgtcgt agcgccggcg ccatcttgag
     4921 cgaaagcgat tcgaggctct cgggatccat gacgacctcg tgccagccca gcaggcgcgc
     4981 ctgcgggccg aggtgttcgt cgaaccagtc ctcgagctcg agtgcgcggc ggcgctcatc
     5041 aggatcggcc gggtagagcg gcggatcggg ctgagcctcc tcgagcgcgg cgatgatggc
     5101 cgtcgagtcg ccgatattcc gcccgtcgat ctgcaggacg gggaaggtcc cgcactgccc
     5161 gcgagtcagg gcgaacgcca cagcaggatg ggtccctgca accgccacgt gccgatcgtg
     5221 cgcgatgccc ttgaaggcca gcgcccagcg ggccttctcg ctgtagtgcg agaaggggat
     5281 gtgccacagg accggcttgc cgttaccgcc caccgccgct gagcgtaaag aacggtgcct
     5341 tgcattgcgg cggttgatgc gtaatactga accgtatggt tcagtattcc cctgtcgacc
     5401 agacgctcgc agccctcgcg gaccccactc gcagagcggt cctcgagcgg ctcggcggcg
     5461 gcagcgcgag catcagcgag ctcgccgagc ccttcggcat gtccctcacc gggatgaaga
     5521 agcacgttcg ggtgctcgag aacgccgagc tggtctccac cgagaaggtc ggccggacgc
     5581 gtcactgcac gctcgggccg cggcggctcg aggacctcga gcagtggatc gccgactacc
     5641 ggcggatgct ggacgaacga ctcgagcggt tcggggagct cctcgaacgc cagaaaggag
     5701 tgtcatgacc agccaggagc agcaggccca ggcagccacg atcaccacgc cgagcgatcg
     5761 cgagatccgc gtggagcgcg tcttcaacgc accgcgtgac caggtgttct cggtctacac
     5821 gaaccccgac ctgatccccg agtggtgggg tccgcgcggc atgaccaccg tcgtcgacga
     5881 gatggacgtc cggaggggcg gccgctggcg cttcgtgatg cgcgagccgg acggcagcga
     5941 gtccggcttc cacggcactt accgcgaggt cacgccgccg gagcggatcg tgcagacctt
     6001 cgagtgggag ggcatgcccg gccacgtatg tgtcgagacc gccacgttcg aggacctcgg
     6061 cgaccgcacc agggtcacca ccgtgtcgat cttccacacc accgaggagc gcgatggcat
     6121 gctcgcgtcc ggcatggaag gcggcctgaa cgagacctac gcgcgcctcg acgagctgct
     6181 ggagcggctc gccgcgcgct gatcagcccg aaacgcgagg aggcccgcaa aagcgggcct
     6241 ccttggttct tcgggcgcgc gtcagtcctt cggcgcgtag cggtgatgga tcgcgagcat
     6301 gttgccgtcg ggatcctcga agaacgcctg gaagcagacg cccgagtcga tcgtgtcgcc
     6361 cttgaagctg acgccgcgcg actcgagctc cgcatgggcg gcatcgaagt cgtcgacgtg
     6421 gaattcgacc gggtggctgt gggggcggaa ctcgagcccg aacgcatccg actgcatcac
     6481 agcaaccgtc aggtttccgg tctcgaactc ggcgcccggc atctgccccc agcgcttgcc
     6541 ctcgggcagg cccaggacac cgccgtagaa ctcgactgcc ttgtcgaagt cccgggtcgg
     6601 gatcgtgatg aagtcggtgc cggtgatcag ggtcgtcatg aagctggctc ctccgtcggg
     6661 gtctgggcac gtgtatgaag cgtgcttacc agagcagccg tctcccatgc tgctcctggc
     6721 gctcgagcga ctcgccgatc aggtacagcg tcggcggcca gaggccgacg aagagcgctc
     6781 gccgctccgc gtttccgcgt tcgtcctggt cgaccgtttt cgcccggatc cagagaacga
     6841 tgcacaggcc cacggatccc atcgaagcga tttgcaggtg ccagcctcgc atccccaggg
     6901 cgtagagctt ctgtatcagc acgaaggctc ggtctaccca tcgcgcgcgg gttcaaccac
     6961 agggtgctac gcgcctaccg tcgggtagga cgaggtcgtg cctggactcc cgctctcgcg
     7021 acgcctggcc accgccgccc gctggccgat cggcgtcggg ctcacctcgt ggcggtacat
     7081 gtggcgtacg acgcccttcc atcgaggtga gcgtgcgggc tcgcccgcgg aagaccttgc
     7141 gccgccgctg cctgagaatg tctcacgcga ggcgatccag ggaccggagg acggcatcgg
     7201 tcccctcttc catcgcaggt acggtgtgcg agtcagggat ccgcacatgc ccgcccgcga
     7261 gctgatgtcg cggctgcaag ccgacccgga tgctgcggct cccggcgagt tcgcaacgtt
     7321 cataaagctt cagggcgccg agggccggat gcgcgtcggc gacgagttcg tggttcgcat
     7381 ggccgggccg tgggacgggc cggtccgcgt gatcgagacg accgacacct cgttccggct
     7441 ggcgacgctc tcacgccatc tagaggccgg tcaaatcgag ttctcggccg ccgacaccga
     7501 cgccggtctg gagttccgga tcgagtcgtg ggcgcgcagc gcggaccgcc tcgcggacat
     7561 tctctaccag cacctgcgca tggctaagga aatccaactg cacatgtggg cctccttcct
     7621 cgagcgcgtg gccgagatcg cgggcggccg ggtgaccggc ggaatcgcta tcgacactcg
     7681 ccgggtagaa gctcccgtcc agccggcggt cgcgcacctg agacgcctcg cattgaactt
     7741 cgaccccgag tgctatgagc agcatgtgcc ggagaacggc tggcatgtcg acgacctgcg
     7801 cacccgcctg ccgccggagc ctccaggccc gcccgtcgaa ggaggaagtt gggaggtcgc
     7861 ccggcggctg atgcgtggct acgagttcgc agacccgtcg atcgtccggg cgagctatga
     7921 ccctgacgag ccgctggagg gacgcaacat gcttctcgag ctgcgcttcc acggattgcg
     7981 cttccacggc ggcgtccgcg tctcgcaggt ctacgacgag gaggtcacgc gtgaccgccg
     8041 taccgcccgc gtgtggggct gggcctatca gacgcttgaa ggtcatctcg agatgggcca
     8101 gatggactgg caggtttgga agtggctcga caccggcgag gtggagttcc gcatccacgc
     8161 ctactcccgc cgcgcccgcg accggaatgc gatcgtccgc ttcggcttct ggttgttcgg
     8221 ccggcgcgag cagctggcgt tcctgcacag cactctcgaa cggatgcgcc gcctcacaga
     8281 gactgctctc gaaaaaggag gcggagaacc ggtccgtaag acggccgaac agctgacagc
     8341 gcgccccgct aggggcacag cgcgcgcaca ctcaagactc gcggcgtatc tggaggacgg
     8401 ccacgaacac agcactccaa ctgagccgcc caatcgggcc tgagccaggg cctcgcccca
     8461 ccgacgagat cacgtggtga gcccggtcgg cggcgagata tagcgacgat cggcggtcgt
     8521 ctcacgcagg gctctgcgga gcggcacgtg ctgctacctg taatcggggc gactggattc
     8581 gaaccagcga ccgctcggcc cccagccgag tgcgctacca gactgcgcca cgccccgaag
     8641 cgggcgacgg gaatcgaacc cgccctagaa gcttggaagg cttctgtgca accacaacac
     8701 ttcgcccgcc gggtgaggcc attctagagc gatgaacggg cggcgacacc tcgggatggc
     8761 gcgagcccgg ctcgataccg actgatagcc gcgcgctttc ctgctaggcg gttttcgtat
     8821 tgacccggca catcgggcga ccgatcatcg cccgtcatga gcgaagaaaa gaagccaccg
     8881 cgaagcacca acgcctcggg cgttcccgtc ggaagcgacg agcactcact caccgccggc
     8941 ccgaacggcc cgaccgttct gcacgacgcc tacaccgtgc agaagatgca gcaggtcgcg
     9001 atcggcacga ggcgtcgcac gccccgggta gtcggccgct acgcggggat ccagcaacga
     9061 gcggaagtgc gtcaaattca cccgaaacgg gtgctggacc cggtggtgcc tgtcgattag
     9121 gttggcacga tgctcgccga agcgatcacc ttctcggccc ttctggtcct gaccgccgcc
     9181 gccgcgccgt acctgcgttc gcggctgccg ctgtctgccg acaacgtgat tcgcacgatc
     9241 atgcccgctc ccctcgtgct catggcatcc ggcacgcttg ccgtcggtga ccatggcgag
     9301 gccgcgctgg cagttgccta tgtgggggtg cagatgaccg ccatcgcgtg tctcttcgtg
     9361 cccacaagac gatcgcgctg gtcgcgcttc gagcgccagt tcagggccta tgcgacaaag
     9421 gccgagcacg tccgccagta gcggacgcgc ccggctgtcg agaatccgga ggcaaccccg
     9481 ccctaggcgg tgctgtccgg ctccgtctcc tcgtcgcctg tcgagccgag gggcgctgcg
     9541 cggcgccctg aaccgcctgc tgcaacctcg gccggggtgt cgcctggctc cgccggcgtc
     9601 ttctccgcgt tgaagcgctt gccggtgtca cgcggctttg aggtcttgcg tgtgccgccc
     9661 ttgcgcgctc cgggtgagtc cgcgcgtgtc ctcgccggag tcttcggaag gtggaggttc
     9721 gattcaatgc cggtcacctc cggaatgccc cgcacgatcg actcgatctg cctgatgcga
     9781 gtggggttct tggcctgccc gtgaagcacg acgacgtggt cgaccacgtt gatgttcacg
     9841 tggcccttga cgtccggcag ccgccggaag agcacggtct cgaccttgcg cgcgagcgtg
     9901 acgtcatcca tgtcgggctt gggctccgac cggaggtgac ggacccgctg caccgtgccg
     9961 tccacctcgc gggcggcacg cctgcccagc cgttcgagct ccttggaggt gcgtcgcacc
    10021 gctcccaggc ctctgtccgc caagggcttc agctgtccca acatcgccgg cctcccagat
    10081 tccgcttgct cggatggggg ctgcccggcg cccgtgccca gccaaacgcg gaaatggagg
    10141 accggggccc ggctgccgct ctacgcgaca gccggggccg gcgccggctc gagcgcgagc
    10201 tcgagcacct cgccgatcgt catcaccggg tggaacgtca tctggtcgcg gacgtcctca
    10261 ggcacgtcat cgaggtcgcc gcggttgcgc tccggcagaa tcacgtcggt gagccccgcg
    10321 gcgtgtgccg cgagcacctt ctgcttcagc ccgccgatcg gcagcacccg gccctgcagc
    10381 gtgacctcac cggtcatgcc gaccgtgtgc cgcacggggc ggcccgacag cagcgacgcg
    10441 agtgcggtgg tcatcgtgat gcccgcactc gggccgtcct tcggcgtggc gcctgccggc
    10501 acgtgcacgt ggaactcgcg cttgtcgaac tcgtcggggt cgatcccgag cgcgtccgcg
    10561 tgcgagcgca cgtaggagag ggcgatccgc gccgactcct tcatcacttc gccgagctga
    10621 cccgtcagga cgaggccgtc gttgccgcgc atcccggtcg cctcgacgaa cagcacgtcg
    10681 ccgccgacgc cggtcaccgc gagaccagtg gccacgcccg gaacagccgt gcgctcggcc
    10741 gcctcctggt ggaagcgctg gcgtccgagg gcgtcgcgca cctgctcggt gccgatctcc
    10801 acgggtgccg tcgcctcacc ggacgcgatg cgagtggcgg tcttgcggag cacgcgcccg
    10861 agctcgcgct cgaggttgcg cacacccgcc tcgcgcgtgt actcggtcac gacctgccgc
    10921 agcacgtcgt ccgacaccgt cacctcgtcc tcgttgaggc cgttgcgctc gcgctggcgg
    10981 ggccacaggt agcccttggc gatcgcgacc ttctcctcgg tggtgtatcc gtcgaagcgg
    11041 atcgtctcca tgcggtcgag cagcggcgcg gggatcgtgt ccgccacgtt cgccgtcgcg
    11101 atgaagagca cctgggagag gtcgatctca acgtcgaggt agtggtcgcg gaacgcgtgg
    11161 ttctgcgcag ggtcgagcac ctcgagcagg gccgccgaag gatcgccgcg ccagtcggcg
    11221 ccgaccttgt cgacctcgtc gagcatgatc accgggttca tcgtgtcggc gtcacgcagg
    11281 gcgcggacca gccggcccgg cagggcgccg atgtaggtgc gccggtgacc gcggatctcg
    11341 gcctcgtcgc gcacgccgcc gagcgacatg cggacgaact cgcgacccgt ggcgcgcgcg
    11401 atcgactcgc cgatcgaggt cttgcccgtg ccgggcggcc cgatcagcgt gaggatggcg
    11461 cctgagcgct tgtcctcggc gatgccgcgc tcctcgcgca gcttcttcac cgcgatgtac
    11521 tcgacgatgc gctgcttaac gtcctcgagg cccgcgtggt cggcgtcgag cacctcacgc
    11581 gcgtgcacgg gatccaggcg ttcgtccgag cgcttgctcc acggcacggc gatcagccag
    11641 tcgaggtagc tgcggatggt cgagctctcg ggcgactgct cgcccatgcg ctcgagcctg
    11701 ccgagctcct tgtcggcctg ctcgcgcacg tgctccggca tgccggcctc ttcgatcttg
    11761 gcgcggtact cgtcgatcac cgagccctcg tcctcgccga gctccttctg gatcgactcc
    11821 atctgcttgc gcaggaagta ctcgcgctgc tgcttctcag cgccagagcg cacgtcctcg
    11881 cggatgcggt cgcgcacctg ctgctcggcg agccgctcgc gctggaactc gagggccagc
    11941 tcgagccgct cggtcacgtc gagggtctcg aggagctcgc gcttctgatc gaagccgaga
    12001 tcgggcgagt agccggcggt gtccgccagc gcgcccggct caacgatgga gcgcacgaaa
    12061 gccgcaatgc ggccgtcgtc gccgcgcagc tcgaggatct cctcgacgac ggcgcggtac
    12121 tcgcgctcgg cgttacgggt gcgcccgtca acaggcgtgt cgtcggggtg tggctcgacc
    12181 tgcacgcgga ggcggccgtc cgggccggtc gaggccgcac cgatgatggc gcggtgctga
    12241 ccgaacaggc tgacggcctg ggccccgccg gggaggcgaa ccttctcgac cacttcggcc
    12301 accgtgccga cacggccgaa ctcgttctcg tgccggggca cgagcaacac tcgatcctcg
    12361 tcaccggtgt cgatggcgag ggtcacgttc atgttcggga agacgaccgt gtcttccagc
    12421 ggtatcagga tcaagttctc caatgtggtg ccttcgttgg tggtactcac ggaaagtaag
    12481 tttagcggcc tggacttaga tcgtacgggc cctcggattc cccgtggcgc tgcggctgac
    12541 cgccgtttac agcggcaatg aggggatgga cgaggggcgg cggggtgtcc gcgaagccgc
    12601 gcgtaccgcg cttgggttac ctcggtctag gcgtgggttg accttttccc tgcataccgg
    12661 ggacaaagtc acccacgtcc tcctgcctac cgcgcttggg ctaccgcgtc ctaggcgtgg
    12721 atgacgttac tcgctcatag ggcgagaaaa gtcacccacc ccctcgcggc cagcccttaa
    12781 ttgccgctgt agacgctcgc actaaggagc cgcggtggcg cggatgcgca cccgcgggcg
    12841 cgcattgttc acgtagccct ttgggttcca cagcgcagcc tcgtcctccg gctgagtggc
    12901 ggccgaggag tcccaggcgc ggacgacgat ctcgtgctcc ccaggctcca gctcgaccgc
    12961 ggtccgccag tgccgccacg cccatcggcc gaggtcgtcg agcagttccg cctgcgacca
    13021 gctcgcgcca ccgtcgagcg agacgtcgac acgcgcgaca tgtcgctccc cgcccgcgaa
    13081 cgcatagccg cgcaccacaa ccggaccggc cgtgacggtc gcgccgtcgg ccggagacag
    13141 cacgtccgca ttgagcgcga ccaggccgag cggcatcccc gcgcccgggc ccggccttcc
    13201 gtcctccggc aagagccggt aggcgacgtc ctggaagtaa ccgtgccagg gcgtcgagcg
    13261 cacctcgatc cgctcgagcc acttgacgct gcgtgcgccg atgtagccgg gcacgaccac
    13321 gcgcagcggg ccgccgtgga gcggcggaag cggctcgccg ttcatccccc aggcgagcag
    13381 gacttcggga cggcacgcct tgtcgagcgg gattgacccg ccaaagcgct cggagggctt
    13441 ggcctgtgag cagagatcgg cgccctcgaa gcccacgtgg gcggcctcgc gaagcgggcc
    13501 ggcaagcgcg agcacgtcga cgagcgcgac tcccgtccaa gtggcggtgc ccgtcgcgcc
    13561 gggaccccat ggcgcctcgc cgggaatgtc gcggatggag acgaggcccg cgcgccggtt
    13621 gcccgcgcac tgaagcgtcg ccgtcagctc ccgctcctgg aacgcctcgc gcagcgtgcc
    13681 aatggacacc tcgagggtgc gttcgacgag gccgtggacc cgcacatgcc acgcggaggg
    13741 gtcgagatcc ggcaccgggc cgtgcccccg cacgtagaac gcgtccgtcg cggtcaccgg
    13801 accctcggca agggcggccg gcggagtctc ggcgttgaac ggctccgcct ggtgcacgat
    13861 cagatccgcc cgctttccgt aggggctcac caaagccggc gccggcagct gcgtatgcat
    13921 ggttccgctc ctcggggcga cgctacgccc cgccggcccg agggtcaaga agggcgatgt
    13981 agtgcgcccg agctatgcgg cagccgctgc cgctcgggcc cgcttgacct cgtacatgcg
    14041 cgcatcggct gctcgctgga ggtcatcagc ggtctcaccg ttgaccggga acaaggcaaa
    14101 ccccaccgac gccgacgccg gcgcgatcgc cgcctcgagc cgcttggcca gcgcctcggt
    14161 gtcgcgccgt ccggcgccca cggcgacgat cgcgaactcg tcgcccccca tgcggccgag
    14221 cacgtcggtg tcgcgcacca cggaccgcat gcgcttcacg gcctcgacca gcatcgcgtc
    14281 gccggcggcg tggccgttcg tgtcgttcac ggccttgaag ttgtcgaagt cgagctggat
    14341 cagcgagagc gggagctcgt cgcgccgcgc gtccgccagc gccgcctcga gccgctcctc
    14401 cagcccgcgg cggttgaggc aggcggtcag cggatcggtg cgggaggcca gcgccagctc
    14461 gcgccgctgg cgacggccga tgctggtctg ccaaacggaa gccccgctca cgatcgtgag
    14521 cgatccgagg aacagcatca cgtgcggcca ggcggtctca ctcgcgagga acccgactag
    14581 cacgaacgag cccatgtcca cggcggccac cgccgccacc aggcgcgtgc tcgagtagga
    14641 gacgctcgcg aacacaagcg ccacgaagcc gaggtaggag agcgggctgg cgacgccgcc
    14701 gtcgagcgcg gcgcccgcgg cgatcaggac gatgacgccg aggttccagg agagcgtgaa
    14761 cgcgacctcc cgcgtgcgcg tggtgagcag gcggcgcagc ggcacgaacg gcacgagcag
    14821 gctgaatccg agcgccccgc cgatcagcgc ggcgatcgcc gtgcggttcg gtccgtccca
    14881 ggtgctcgcc acataggcgc cggcggcgcc gcacgagagc agcgtcgtcc acacgccacc
    14941 ccaggcgcta ctaaggatcc tctcggttcc gtgggaatcc atccccacgg cgtacatcgt
    15001 ccaaatgggt cactcccctt gagcggtgca aggccgcgag cttcccagcc gtggcgatgt
    15061 cccgcacctg agggcgccac cacgcggcga tccacgtggc cagcatcccg cagaggaacg
    15121 ccgcccacag ggcgatgccc ggcactccga gctccacgaa cgagcgcatg tagtcgttgt
    15181 gcgccgtgaa gcactgttgt gaggaacagg atcctcgccg tcattgacct cgccgtccga
    15241 gcaccagcgg cacggtgcgg agcaggatct tcaaatcaag ccaaagcgac cagttggcga
    15301 ggtagaggta gtcgatcctc accatgtcgc gcagcggcac gcgcgaggag ccgagcacct
    15361 gccacacccc ggtcatgccg ggctggacgt cgaggcggcg gcggagccac ccttgtacgc
    15421 ggatgtcttc ctcgggcacg agcgggcggg ggccgaccag gctcatctcg ccgcgcagca
    15481 cgttgaacag ctgcggcagc tcgtcgatcg acaagcgccg aagcaccttg ccgacgcgcg
    15541 tcagccgggg gtcgtccgcg atcttgaaga agccgtcttc agcctcgttg agcgcggcga
    15601 gctcaccctt gtggctatcg gcgtcctcga ccatcgtgcg gaacttgaga agcgggaact
    15661 cccgggccca gagccccacg cggcgctgac cgaagaacac ggaaccgcgc gagtcgagct
    15721 tgatggcgac ggcgacgagg gcgaacaggg gtgcaagcac gatcagtccg agcagcgagc
    15781 cggtcacatc gagcaggcgc ttcaggcggc gcgaggagcg cgagagctgc gacacccgca
    15841 cgccgagcag ctgcatgccg tctacgtcgt cgaactcgac cgccgagccg actacctcga
    15901 gaagccgcgg cacgagcgtc accttcactc cgaccgcctt gatcaggcgg atcttgtcga
    15961 gcaggcgatc cgatcccgca tcgctccccg gcgcgatgat cacacggtcg atctgctgcg
    16021 cccacagcac cgccgcgagg ccgggcagcg tgccgaggac gggcgggtcg ccggggactg
    16081 tggcgggccg aacactggtg tcgatcccgt gagggcgcct gccctcgagc gggacgcgcc
    16141 cgacaacgat cgcgtttacg agcttgcttc gctccgtacg gcgcatgagc tcacgcgcgg
    16201 caacggcatc gccgagcacg aggcagcgct cagggagagt gacggcaagg cccaagcgtc
    16261 tcgccgcgat ccggttcgcc acgagcgcgg cgaagagagc ggcgagcacg cccaccacct
    16321 cgagtcgatc gaggcgaccg tcgaagaccg ccggcgacgc gagccacgtg agcagcgtga
    16381 aggcgatggc cacgtggaag agcgaggtgg cctcgtcgag cgtgcccttc ccgagcacgt
    16441 gctcgtcgcg gtcgtagagg ccggccgtct tgctagccag cacgacgaga ggcaaaagtg
    16501 ccaacgcacc gggttggagc gtgtcgccac caactccggc tatgaataca agcgcggcaa
    16561 gcgccgcgag gaggtccgcg gcgaccagcg cacgtttgaa ggtcgcgtcg cgtccgagta
    16621 ccgcgacgtc gcgatcgccc tcgcggatct cgaacgcggg atcggggcgt cttttgaaga
    16681 ggacaagcga cgatcgtcgt ccccgaaacc ggcccggtcc gatttcttcc cttgtttgcc
    16741 ccccgggggc gtctgtgtac ccctccgccg gggccctgcc gagcccgaca cccatcggcg
    16801 gcctcccgta ccgtccgaag tgcgagtttg cgaatttagc gcacctcgag agccggcgcg
    16861 cagcttcggt gtgaacggcg ccgtacgagc ggcagccggg tacattgcgc cccccatgga
    16921 gctggcgggg agcgcttccg cgcgcgcgtc cgagaggggc gagacgcggc cggccgtgga
    16981 tgtggtggtg cccttccacg ggacttctca ggagctcgcg gatccgcgcg tcaggctcgc
    17041 caagctacgc ccgcgcgacg gcgactcgct cacgatcgtg gacaaccggg ccgacgcgga
    17101 gggcacggca ctcgggggtc aacggagggt cgtggccgcc cgcagcgcca gtcctcctac
    17161 ctcgccccgc gctcagccac gcggtattga tcgcgggcaa ccggttgcgg ttgctcgcgg
    17221 tcgcgcgcac ctgtgcccgg cggctcgcat tcaagcgcag gctccgcgcc acggcgaacc
    17281 ggcgtaccac ccgaagcgct tccatatgat cgcgcctggt ggggggcttg gcgggcattt
    17341 cacagggcgg ggggcgcgcg tggcggagga tccgcttcac gctgtgggca ctgcggctgc
    17401 ggggtcggct cgcccggcac ggctgccgcc tcgacgtttc ggcgcctcac ggcgcgccgc
    17461 ttttcgagcg cacccccgtc gtacggctgc acactgaggg tgacggccgc ggcagcctca
    17521 cgctgcggtt cggcgcgggc gtctcattcg ggcgcgacgt ggtgctcgag gtgtgggcgc
    17581 gacaggcgaa cgtgctggag ctcggggccg gtacgcgcct cgaggaccgc gtgcacgtgg
    17641 ccctgcgcgg cgggcacgtg cacctcggct cggagtcgac cgtccacgag ggcggcgaga
    17701 tccacgtgga gggactgttc atcaccggtc gccgcgccta cgtaggcagc ggcgcggtgg
    17761 tgcgctgcac gcaacggatc gagctcggcg agcgcgtgac tctggcagag cgctgcagca
    17821 tctcggacct cggccacgcg caggcgggca gcggttccaa cggcggcagc gagccggtca
    17881 tcgagcccgt ggaaattccc tcggacacgc tcgtcgggcc caactccgtg ctcacccagg
    17941 gcgtgcgcct gggcgagggc tcgattgtcg aggccggcgc gctcgtttac ccc
//
LOCUS       DASVXS010000007         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_708627, whole genome shotgun sequence.
ACCESSION   DASVXS010000007 DASVXS010000000
VERSION     DASVXS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708627"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VF752_00335"
     CDS             complement(<1..562)
                     /locus_tag="VF752_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8854019.1"
                     /translation="MRVLVTGGSGFVGSHVVDRLNAHGHEAVIFDLVPSPWHPDVDAV
                     IGSLMDAGALDEALDSCDAIVHMAAVADVREVDREPGHAEHVNTRGTARLLEAARRNG
                     VERLAYASTIWVYSDCEPQEVDERTPVIPPSHLYTATKFAGELYCKSYSEAYGIETTV
                     LRLGIPYGPRAREAAVIPTFVGKALRG"
     gene            complement(559..1590)
                     /locus_tag="VF752_00340"
     CDS             complement(559..1590)
                     /locus_tag="VF752_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8854020.1"
                     /translation="MTRPAFPRPAPRASGLGSFRIGFDERDRVRLHELWDGVIDSGRW
                     SEGELTARFEQKWKALHRAEAVAMSSWAGGALAALEFAEVRGKPVLCPSNTFMATPLA
                     IIKAGGRPVFVDCNREDLCMRFEDFERTADAAKPAAAVLVHIGGHIAFESERIADYCR
                     EHGIFLLEDCAHAHGATWNGRRAGTYGDAGVYSMYATKTVSTGEGGVLVTRRPELAEF
                     ARAYRNYGKPSYEVQGLNFRMSEFTAAIGLVQAERMDEIVAWKNTAARTHLDPVHSAR
                     IELPEGMTSGLYKYIVFEPIERSTGRVYDEPCHRVLGDPSDLPNTDWVAQNHWCVPLY
                     YHGGGEVAE"
     gene            complement(1662..>2693)
                     /locus_tag="VF752_00345"
     CDS             complement(1662..>2693)
                     /locus_tag="VF752_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8854021.1"
                     /translation="AARDELLGATVTLAGQSDAAPRLCLDGHVDVVDPGTAPWQFDPW
                     SGDEVDGCIRGRGAADMKGGVIAALHAMAAVRAALGAAPGEVVLYGVSSEEDGGLGTF
                     AALERDDRFDACLIPEPTGFDIVCAQAGALTFEGVVPGVSAHAALRLEGESAIDRYVP
                     IHAALHEHERRVNANVDHPLMRELELPYPLLVGRLEAGTWSSRVPDRLRFEGRLGVQV
                     DQTPGEARGAFEAVVRQACPDAELGWTGGQFAPAETPLDHPFPQLVASVIEEETDRRP
                     RFIGSSYGSDMRLFCERGIPSVMAGTAGIELAHAVDERVSLSEVVALARSIARLIVGF
                     GELASAASS"
BASE COUNT          399 a          979 c          910 g          405 t
ORIGIN      
        1 cgccgcgcag cgccttgccc acgaaggtgg gtatcaccgc cgcctcgcgg gcgcgcgggc
       61 cgtacgggat gccgagccgc agcaccgtcg tctcgatccc gtaggcctcg gaatacgact
      121 tgcagtagag ctcgcccgcg aacttggtgg ccgtgtagag atggctcggc gggatgaccg
      181 gcgtgcgctc gtcgacctcc tgcggctcgc agtcgctgta cacccagatc gtgctcgcgt
      241 acgcgaggcg ctcgacgccg ttgcgccgcg ccgcctcgag cagccgggcg gtgccgcgcg
      301 tgttcacatg ctcggcgtgg ccgggctcgc ggtcgacctc gcgcacgtcg gcgacggccg
      361 ccatgtgcac gatcgcgtca cacgagtcga gcgcctcgtc gagcgcgccg gcgtccatca
      421 ggctgccgat caccgcgtcc acgtcggggt gccagggcga cgggacgagg tcgaagatga
      481 ccgcctcgtg gccgtgtgcg ttcaggcggt cgaccacgtg cgaccccacg aagcccgatc
      541 cgcctgtgac gagcaccctc actccgccac ctcaccgccg ccgtggtagt agagcggcac
      601 gcaccagtga ttctgggcga cccagtcggt attcgggagg tcgctcggat cgccgagcac
      661 ccggtggcag ggctcgtcgt agaccctgcc ggtcgagcgc tcgatcggct cgaacacgat
      721 gtacttgtag aggcccgagg tcatgccctc ggggagttca atgcgggcgc tgtggaccgg
      781 gtcgagatgc gtgcgcgcgg ccgtgttctt ccaggccacg atctcgtcca tgcgctcggc
      841 ctgcacgagc ccgatcgccg cggtgaactc gctcatccgg aagttcaggc cctgcacctc
      901 gtagctcggc ttgccgtagt tgcggtaggc gcgcgcgaac tccgccagct ccgggcgacg
      961 cgtgacgagc actccgccct cacccgtcga gaccgtcttc gtcgcgtaca tcgagtagac
     1021 gcccgcgtcg ccataggtgc cggcgcgacg cccgttccac gtggcgccgt gcgcgtgggc
     1081 gcagtcctcg agcaggaaga tcccgtgctc ccggcagtag tcggcgatgc gttcgctctc
     1141 gaaggcgatg tggccgccga tgtgcacgag caccgccgcc gccggcttcg cggcgtcggc
     1201 cgtgcgctcg aagtcctcga accgcatgca gaggtcctca cggttgcagt cgacgaacac
     1261 cggccgccca cccgccttga tgatcgcgag cggcgtggcc atgaacgtgt tcgacggaca
     1321 cagcaccggc ttgccccgga cctctgcgaa ctcgagcgcc gcgagcgccc cgccggccca
     1381 gctcgacatc gcgacggcct cggcgcgatg gagggccttc catttctgct cgaagcgtgc
     1441 ggtcagctcg ccctccgacc aacggccgga gtcgatcacg ccgtcccaga gctcgtgcag
     1501 ccgaacgcgg tcgcgttcgt cgaagccgat tcggaaggag ccaagccccg atgcacgcgg
     1561 ggctggtcga gggaatgcag gtcgagtcat agccgcgccc actcccctcc ggaccggaca
     1621 ctacacggct ttcccgcgct cagccggact tcatcaacgc tttagcttga ggccgcagat
     1681 gccagctcgc cgaagccgac gatcagccgc gcgatcgagc gcgcgagcgc gaccacttcg
     1741 ctgagcgata cgcgttcgtc gactgcgtga gcgagctcga tgccagccgt ccccgccatc
     1801 acggacggga ttccgcgctc gcagaagagc ctcatgtcgg agccgtagct gcttccgatg
     1861 aagcgcggac ggcggtcggt ttcctcctcg atcacgctgg cgacgagttg cgggaacggg
     1921 tggtcgagcg gcgtctctgc gggcgcgaac tggccaccgg tccagccgag ctcggcgtcg
     1981 gggcaggcct gccttacgac ggcctcgaac gcgccacgcg cttcgcccgg cgtctggtct
     2041 acctgcacgc cgagccggcc ttcgaagcgc agccggtccg gcacccggct cgaccaggtg
     2101 ccggcctcga gccggccgac cagcagcggg tacggcagct cgagctcgcg catcaatggg
     2161 tgatcgacgt tcgcgttgac ccgtcgctca tgctcgtgga gggcggcatg gatcggcacg
     2221 tagcggtcga tcgcggactc gccctccaac cggagcgccg cgtgagccga cactcccggc
     2281 acaacgccct cgaacgtgag cgcgcccgcc tgcgcgcaga cgatgtcgaa ccccgtcggc
     2341 tcggggatca agcaggcgtc gaagcggtcg tcgcgctcaa gtgccgcgaa ggtgccgagc
     2401 ccgccgtcct cctcggagct cacgccgtag aggacgacct caccgggcgc ggcgccaagc
     2461 gccgcccgca ccgcggccat cgcgtgcagc gcggcgatca cgccgccctt catgtcggcg
     2521 gctcctcgtc cgcggatgca gccatctacc tcgtcgcctg accagggatc gaactgccac
     2581 ggcgcggtgc ccggatccac gacatccacg tggccatcga gacagaggcg cggagctgcg
     2641 tcgctctggc cggccagcgt gacggttgcg ccgagcaact cgtcccgcgc agc
//
LOCUS       DASVXS010000008        37920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1346524, whole genome shotgun sequence.
ACCESSION   DASVXS010000008 DASVXS010000000
VERSION     DASVXS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 37920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37920
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346524"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VF752_00350"
     CDS             <1..276
                     /locus_tag="VF752_00350"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX8854022.1"
                     /translation="GFIYTVSLAGTTGERAELPPGLAETVRRVRAVATVPVAVGFGIS
                     TGEQARRVAELADGVIVGSRVVRAAGEGGAGAVRDVVAELAAGLTAD"
     gene            313..495
                     /locus_tag="VF752_00355"
     CDS             313..495
                     /locus_tag="VF752_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854023.1"
                     /translation="MAVLVLLVLGLVIWVVGWSITGNGFDFFMITLLLLVIGAAIHIV
                     TPHLRRMLGHEPPPLD"
     gene            524..1294
                     /locus_tag="VF752_00360"
     CDS             524..1294
                     /locus_tag="VF752_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854024.1"
                     /translation="MSPTTKRDPWDSDPLWGDARDRVRGEVQAAFKPKAQTCPSCGRT
                     EETAARNCPHCGSSYVFTRQRGLSKRVRVALGASLAVLVAGIAVALIVFVPKIGDTKR
                     ENAARDRAAEAAFIKRETARLKQDQAPHRARSEAAAPSAVVADLQRSIFADAKARLEA
                     GSFKVPVKTVQCTRITLGPLATGPRGGYDCLAVTSYVSSNGNNQAGALGYPFWAVVDF
                     KRRTYTWCKVNPKPGERGVQSKEPIVPLVPACQIKPND"
     gene            complement(1310..2332)
                     /locus_tag="VF752_00365"
     CDS             complement(1310..2332)
                     /locus_tag="VF752_00365"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX8854025.1"
                     /translation="MGVLTGGGDCPGLNAVIRAIVRKGIDHYGHEVVGFRDGWRGPIE
                     GDAEELTIESVRGILPRGGTILGSSRTNPFKRDDGVDRIRENLTAERLDGLIAIGGED
                     TLGAAAKLHAAGLPVLGVPKTIDNDLGATDMTFGFDTAVQVATEAIDRLHTTAESHHR
                     NLVVEVMGRHAGWIALYSGLAGGADVILIPEKPFDIERVCGLIRRRHARGRYFAIVVV
                     AEGAVPIEGTMEQLGGELDEFGHMRLGGIGQRLEHEIENRTGFETRTTVLGHIQRGGT
                     PTAFDRVLATRFGVAAIDAATDGAWGMMPALRSTKIELVPLEDAVRELHTVSPEEIEV
                     AEVFFG"
     gene            2519..3784
                     /locus_tag="VF752_00370"
     CDS             2519..3784
                     /locus_tag="VF752_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8854026.1"
                     /translation="MRGRFAVSGCLLVAAAVGLTACGGGGGGGGGTKAQGNTLTIYSS
                     LPLQGASRPQSEALVNGMKLALKQANNKAGKWSINYKSLDDSTAQAGKWDPGQVSSNA
                     RKAAQDKSTILYLGEFNSGASAISIPILNRATIGQISPSNTAVGLTADVPGAEPGEPA
                     KYYPTGKRTYVRVVPKDTIQGAALAVVMKDEGCKKPYILNDKEVYGQGLGKNTQNSLK
                     TAGIPALANEGWDPKAPNYRSVASKIAGKGADCILESGITDNNAVQLTKDLASGVPNA
                     KIYGPDGVCESAWADPKKGGIPTSVDSKVECTVATLDPNSYPPEGKKFFADYKATYGE
                     SNPDPYAVYGYETMSLALDAIKRAAANGNDRQAVINALFNTKDRKSVLGTYSIDKNGD
                     TSITDYGLYKVKDGQLNFDRVIKAKGASA"
     gene            3915..4925
                     /locus_tag="VF752_00375"
     CDS             3915..4925
                     /locus_tag="VF752_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8854027.1"
                     /translation="MRTFLSRYGLILVLLILPVAYGIHDLQVDGNLTRLGENLKDGIS
                     NGAIWALVAIGYTLVYGIIELINFAHGDVFMIGSFVSFSFWSTLGLTLTSKGFGLFFG
                     LLITLVIAMLACGTLNVLIERVAYRPLRNAPKLAPLITAVGFSFILQNVGLEWFGGSQ
                     RGVDDLINAQKTFTTIGGVTITHADVLAVAVTAPLVALLAWFVTSTRLGKAMRATAQE
                     PEAARLMGVNVDTTISLTFLLGGIMAGAAGLIYALYQTTIWFFQGFQAGLIAFTAAVM
                     GGIGNIQGAVLGGLIIGCIQQISDNRIGGEWTPAIVFAYLVLIMVFRPQGLLGEETRE
                     AG"
     gene            4922..6103
                     /locus_tag="VF752_00380"
     CDS             4922..6103
                     /locus_tag="VF752_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8854028.1"
                     /translation="MSAIAAGLRDSRERFNQLPRRTIWAIGAVVLVLLILLPFAYQPA
                     SGFIDDCVTALAYVVMALGLNIVVGFAGLLDLGYVAFYAIGAYVIGWYGSGFFASING
                     SKGFHFVAESASHLPGIHINFLFILVIAALFAALAGTVIGLPTLRLRGDYIAIVTLAF
                     GEITRVFAVNGTDIHPFGKNYPLTLGRQGITPIDPIDLPGLKAFDPLNLRPWYWFGLA
                     LVLLVLFVNFRLRDSRLGRAWIALREDEVAAASMGVPLVKTKLMAYATGAAFGGMAGA
                     FLASYQNTVNADQFEFSFSIFVLAMVILGGLGSIWGVVLGAVALSFINSRLIPDVLNG
                     VPHKIGLNFDLTELSFGIFGFLLVIMMVLKPEGLLPERRRQIEFEEGVGADEALYEAR
                     A"
     gene            6100..6948
                     /locus_tag="VF752_00385"
     CDS             6100..6948
                     /locus_tag="VF752_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854029.1"
                     /translation="MSDGATTSQPDRTAPAGANGGDGGSALILQSVAVGKVFGGLVAV
                     DNVDFEIPRHSIVSIIGPNGAGKTTFFNMLTGLYKPSSGRIAFEDGDITGMRPDKITA
                     LGVARTFQNIRLFATMSGVENVMVGQHARMRAGLFGSILRLPRVRSEEARVEEHAREM
                     LTYVGLRPGLHDQMAANLSYGDQRRVEIARALASDPKLLLLDEPTAGMNPNESARLTD
                     FMETLRSDRGLTILLIEHDMKVVMGVSDRITVLDHGEKIAEGPPQEVRSDKRVVEAYL
                     GKQEGE"
     gene            6957..7658
                     /locus_tag="VF752_00390"
     CDS             6957..7658
                     /locus_tag="VF752_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854030.1"
                     /translation="MLEVNDIHTFYGNIEALKGISLEVNEGEVVTLIGSNGAGKSTTL
                     RSISGLSPPREGSIRFEGNEIGETPPQDIVRLGISQAPEGRKCFPRMSVRENLELGAY
                     LRRDARVSDDMDRVFDLFPRLKERERQKAGTMSGGEQQMLAIGRALMANPKLLLLDEP
                     SMGLAPVLVDRIYETIAEINKQGTTILLVEQNANYALDVSNRAYVLETGAIALADDSA
                     SLRENPEVQKAYLGT"
     gene            7655..7945
                     /locus_tag="VF752_00395"
     CDS             7655..7945
                     /locus_tag="VF752_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854031.1"
                     /translation="MTLVFALLGAKALYLTYVWLLSTIAASWLSDRKGYGTKPGLASG
                     LLLTFVGTLIWLIIPARASSPWKVQGPIPKRGGQTVAQARAAQHASESDRQS"
     gene            complement(7942..9213)
                     /locus_tag="VF752_00400"
     CDS             complement(7942..9213)
                     /locus_tag="VF752_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8854032.1"
                     /translation="MDLPPGPRAPAAWQTIAWMARPAAFLRRVHERFGDPATIRTYWT
                     EQPMVLFSDPDAVRDVFRLDPDIAPAGQSWEFLRDFAGRHSILVLDGEEHLRERRLLQ
                     GPFHGERMRAFAPLIGELARRELGTWSGRVTTLDRTRHLALETILRVIFGARDDEEVA
                     RLRTAVETTLDDVRSLPRMLAMALVQRDFGPRSPWGRFRLAVERFDALLVDTVARRRA
                     QPNGDSVLDMLLEQRDEDGNPPSDRHLRDELVALLMGGHDTSAATLAWAFERLARHPD
                     VVVRMREGDPAYLDAVVKEVLRIRPALTIAPRLLLEPVEIAGRRVPAGVQVAACIWLA
                     LRREDLWPQASAFRPERWLEGEPPNPMSFIPFGGGVRRCAGAPFAEMELREVLQVAAD
                     LTFRPVRREPEQPRRSLLVVVPDRGGEILVG"
     gene            9264..9908
                     /locus_tag="VF752_00405"
     CDS             9264..9908
                     /locus_tag="VF752_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8854033.1"
                     /translation="MFGVVALTALLLAASQSFQSSIEPLPRPVQAQLKSAHVWQKGCP
                     VPLSGLRLLTVSYRDFNGRTQTGQLVVNKAAARPLSRVFRKLYRLHFRIRHMQLDDVY
                     GKPSERPRDNDVTGSFECRQAVPSPCTGGRGTGSWSMHAYGLAVDINPRENPYVGCGQ
                     SHDPAARLYRDRSRHRRGMVTAGVIRAFRSIGWGWGGSWSGNTKDYMHFSSTGH"
     gene            complement(9905..10819)
                     /locus_tag="VF752_00410"
     CDS             complement(9905..10819)
                     /locus_tag="VF752_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX8854034.1"
                     /translation="MRIAVTGATGMIGRRVVELLLERGDEVTAFTRDQSRLEEVFGER
                     VQPAVWREPESQPAPAQALADRDGVIHLLGENVAQRWTADAKRRIRDSRVLGTRNLVA
                     GMRDVQPTPSVLVSQSASGYYGPRGDERVDETAPPARGDFLADVVVDWEAEAARAEEL
                     GVRVVRTRTGVVLSPKGGALDRMLPFFKAGLGGPVAGGRQYVPWIHVDDVANAMLFLL
                     DNEAASGPVNLAAPEPATNLELSKALGRALHRPAFAPVPAFALKALYGEMASVVTTGV
                     REVPKRLEELGYQFSHPALEPALRDVVG"
     gene            complement(10830..12275)
                     /locus_tag="VF752_00415"
     CDS             complement(10830..12275)
                     /locus_tag="VF752_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX8854035.1"
                     /translation="MPVTPSAQFSLTLRVELDRRPGQLGLLSSAIGEAGGSIGAIDTV
                     ETGERKAVREITVFCTDQEHVDRLVAAVQAVEGARLIGVTDRTFELHRGGKLHTGLNS
                     PIKTRDDLSMAYTPGVGRVCMAIHGDRERAFEFTIKANTVAVVSDGTAVLGLGDIGPE
                     AAMPVMEGKAMLFKEFGGVDAVPICLDTKEVDEIVRAVKLIAPTFGGINLEDISSPRC
                     FEIENRLIEELDIPVFHDDQHGTAIVVLAALINACRVTGRRISEIDAVMVGAGAAGVA
                     VAKILLGYGIRSIVACDRFGAIHSGRSDYEEGSMNAPKQWLAENTNADHRDGIPAEVL
                     RGADLFIGLSGPGIIQPEDLGAMNSDPFVFAMANPVPEVPPELAAKYARIVATGRSDY
                     PNQINNVLAFPGIFRGALDVRARRITEEMKIAAAEGIARVVGEDELAEDYIVPSVFNH
                     FVSSCVATAVAKEAERSGATRVHADPLTSTA"
     gene            12442..15036
                     /gene="polA"
                     /locus_tag="VF752_00420"
     CDS             12442..15036
                     /gene="polA"
                     /locus_tag="VF752_00420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX8854036.1"
                     /translation="MSDDRRELFLIDGNSLAYRAFFALPETIATSRGMPTNAIFGFAS
                     MLVKILTDYGPKPTIVAWDAGMSGREEAYEDYKAERRTRPDLLAEQWPHLQPLVDAFG
                     YRNVRQEGYEADDVIATLARRAREAGIEVMVVTGDRDMFQLIEPGIRVMATSRGITDT
                     KIYDREDVIERYGIAPELIPDFYGLKGDTSDNIPGVPGIGDKTAAQLLQEYGDLEGVL
                     SHIDDISGAKRKENLRNHADDARVSKVLATAIDDVPVDVDIEHLVAQEPDRSTLRSTF
                     REFELRDPLRRLEEALGEGEEAAPRQVAAVEISAQAFEVPAAEVARLEGEHAALAAER
                     PEREDGAAGRALEFAAYAGTEEVLVGEAETLGALMLAWGPRPVVAHDWKSIASSEAPC
                     DMPPLEHDTMVAAYLIDPARRKYPLDELADDESISANVEGADGLAANAVITRALAERQ
                     LAAIEEQGLTKLLREVELPLVRVLVNMEHTGVMLDTRRLAQIGETFEARIAALEREIW
                     DLAGEEFTIGSPQQLGEILFERLGLSRKRRGKTGFSTDARVLAAIRDEHPIIEKIERW
                     RELTKLKSTYLDALPELVSPQDGRLHTTFNQTATTTGRLSSTNPNLQNIPIRTEIGRE
                     IRACFVAPAGAKLISVDYSQIELRVIAHVADEGVLKEIFRRGEDVHAATAGEMFGVTP
                     DQVDPGTRSKAKMINFGIVYGLSAFGLSDRLNIPQEEAAEFIERYLARFPQVQKFITD
                     TITSATNDGYVKTLFGRIRRIPELRSNNRNTRQLGERLAVSTVIQGTAADIIKVAMVR
                     CNEALAHEGLKTRLVLQIHDELLFEAPDDEVERACAIVEREMVSAFPLEPPLAVDTGV
                     GQNWLEAK"
     gene            complement(15038..15478)
                     /locus_tag="VF752_00425"
     CDS             complement(15038..15478)
                     /locus_tag="VF752_00425"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8854037.1"
                     /translation="MERVLAVLATACVGGLIAFQPPVNSQLAKHTSVLGAAFVSTAIS
                     AVVMAFLLAVAGGYGELRGLTNTPLGYMTGGLLGAALVSVSLVTVRTLGAGGVVAATV
                     SAQLIVSAVLDRLGILGLDKIGVTPLRMAGFALLLAGTALVTIR"
     gene            15620..15994
                     /locus_tag="VF752_00430"
     CDS             15620..15994
                     /locus_tag="VF752_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854038.1"
                     /translation="MSEADAIRARVPDALRAYGETRATVLEAGVVDQRLKELCARYIA
                     EDDEVVNYSTSDAFDERDRAALDWVHAIAWNSDMADADLWNRLRANFSEPELVELGYF
                     IAFTLGQSHWLRTLGLEKSETI"
     gene            16018..16263
                     /locus_tag="VF752_00435"
     CDS             16018..16263
                     /locus_tag="VF752_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854039.1"
                     /translation="MPNISYYPVEKLEDPELRAYLEHAAAHGTPRPESQAIRAHVPAV
                     LHSFSKNWDAVFRKGVLDHPTKELCRVYVSKTIDCHY"
     gene            16396..16599
                     /locus_tag="VF752_00440"
     CDS             16396..16599
                     /locus_tag="VF752_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854040.1"
                     /translation="MWNPEGADDALWERLRLHFSEEELVELGYFIAITYGQQRWIKTL
                     GIGHGEVLDLTKAGLAPQGEQAR"
     gene            complement(16596..17879)
                     /locus_tag="VF752_00445"
     CDS             complement(16596..17879)
                     /locus_tag="VF752_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX8854041.1"
                     /translation="MTLPWSELRHRLDESRAFNTMRDTPYYRDAVYEQFSETEYSRRH
                     AALRQKMHERELDCVILPGGPSHWSFGGGMLWLSGHWEWHALAAYVVFPIDGEPTLVY
                     AMGGTHIEAVRRETAGALSDVRASRGGRFAEVIAERIHELGLERTRIGLLEVDPRHGD
                     YLPVNQYDVLRRLLPGAELVFTERLMHELLSVHSEEELDCVRAAGRLVQHAMEAIVAR
                     AAPGVAEYELRAAAGSAILEGGGDIDFLIIGSTPMSDPAMVFGNPRPSKRVLSEGDII
                     IMELAAGYRGYSAQIGSPVCVGEPTVEIRGFWEEVAKPGYERMVAEIVPGKPIEALRE
                     AGRFFRDNGVQSRPLHAHGIDFVSDGPHVHAERIDAEPFEEVMRPGMVVMAEPNPITP
                     DGMLGMFVGHTFIVREDGNECVDDFPLELAVARWT"
     gene            complement(17876..19453)
                     /locus_tag="VF752_00450"
     CDS             complement(17876..19453)
                     /locus_tag="VF752_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854042.1"
                     /translation="MWSAFFGSQLVVWMNTVGAATVIATLGGSPTLVALVQTATSLPA
                     VLLAFLAGAVTDIVDRRRLLLATQSWMLISTATAATLTLAGAATPVSILLLTFSLGAG
                     MAASIPTFQSLTPELAGPGRLAAGVALNSVAINLARAVGPALAGVVIAVVSAGALFAL
                     EAAGVAAIIALVLSLRSPGPAADEAPEQVFEAMRAGARFVRFSPPVRAVLVRALVFVA
                     GASAFWALLPVVAFGPLELGASGLGVLVGCAGAGAIAGATLLPRLRRRTTLDLAVSFG
                     TVGLAAAVAVLAFVHEPVLAGVATFAAGAAWLSVLSTLNTAAQLAAPDWVRGRALAAF
                     QFSMQGGLAAGALVWGLVADGFSVEAAFLSAAVVLLVSLAARGRASLIGIEEPDLTPA
                     TAWTEPVLPIEPAAGDGPVLVTLEYRVREDDVLAFAAAMEELGRIRRRDGARQWRLYQ
                     ELEDRACWLETFIVDSWSEHLRQHRRMTVADLDVERRAKGFHVGPGEPETRHMLAAEA
                     ALRASNRVTRTRKRGDP"
     gene            19380..20624
                     /locus_tag="VF752_00455"
     CDS             19380..20624
                     /locus_tag="VF752_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8854043.1"
                     /translation="MAITVAAPTVFIQTTSCDPKKADHSERNCRSRSGAHGPLCDDSG
                     GDVAVVVLFMWEATANACIMLPVTARNAKEQLRVTLRDVARLAGVHPGTASRALNEQT
                     RSLVNAETAQRVLAAADELGYHPNPIARGLKTNRSYTVGVLIPDIENPLFPPIIRGIE
                     DRLDQAGYTPVIANTENDAERERIDFRAMRARQVDGFITATARLDHELLDEVAGVGVP
                     LVLVNRRVEDESLPSATADDRAGVRMAVDLLADLGHEHIAFLGGPTELSTGLQRKEGF
                     IAGMQDRGLKPDPALVATGERFTLDEGQRLCHEAIESGRQFTAIVAGNDLMALGCYDA
                     LAEHGLACPGDVSVVGYNDMPFSDHFNPPMTTIRIPHYEIGRSAADLLLRLLEQGPDG
                     STQLVLEPTLVVRGSTAALDKK"
     gene            20768..21964
                     /locus_tag="VF752_00460"
     CDS             20768..21964
                     /locus_tag="VF752_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8854044.1"
                     /translation="MAVSALIGVGCGGGGGSSDKGTIKVGASLPLTGDFSEPGKAAKQ
                     GYQVWQAIVNEQGGLVDGRKVQMVIKDDASNQNTVVADYNALISKDKVQMLLGTFSSL
                     LNLPASAVAERNRMLYVEPAGGSPEMFSRGFKYLFFAQQATADHQGDVFSNYVKGLPP
                     DQRPKTAAYPTLDDPFAQPTSQGIEKILKAAGIKTVYRKTYTIDQKNFDSIANSVKAS
                     GADLVVNGATFEDGVGFVRALLKIGYTPKMLYQTTAPSLGDQYAKALGKGNTEGIFYA
                     VSHSAKAKTPGNEQFVAKYKQMFGGTEVPEDAADAYATAQVMQAAVKAVGGIDDQAKL
                     ADWLRNNKVDTILGPLEWDEAGRPQGQFLVGQWQNGAPEIILPKGATATTQSIVPEWK
                     PSGAGT"
     gene            22021..22902
                     /locus_tag="VF752_00465"
     CDS             22021..22902
                     /locus_tag="VF752_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8854045.1"
                     /translation="MTEFVQTLILGLLVGGVYALLASGLTLIFGVMNVINIAHGAFLI
                     LAAFITYSIWRAIGLDPLAGILITTPVMFVFGWVVYNLTLRRIRTGPAAATVLLTFGL
                     ALVVEGLMGIIWGNTAHAVTAPYFNESFHIGSLFLPKAQVYGGVLALVVLAVLWIVLT
                     QSWLGRAIRAAAVNPQGAALVGVNVSAVSAATFAVGVASAGAGGSIASVLYTFLPGSH
                     YVWISRLLGIIVLGGMGSLPGALIGALTLGIAETMTVTYISPSWATAVPYALVFVVLL
                     VRPYGILGTRLREDVVT"
     gene            22899..24767
                     /locus_tag="VF752_00470"
     CDS             22899..24767
                     /locus_tag="VF752_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX8854046.1"
                     /translation="MTRAKMLSAPAAAWSSWTPPEWVSSRRLRYGLIGAAALFIAAYP
                     LLSDDRYYQNMIILSLVFAIGATGLNIITGYGGYVSLGQGAFIGLGGYTAAILSTKVG
                     GNPFVWVPVAGVVAGLFAAALGVIAMRARGHTFVILTIVFLFLLQIVAINWRELTNGT
                     AGLTMPLPRWSPDIANWPFYYALLGLLVVSIAMSWWIRRTKFGMGLVSIREDEDKAET
                     VGINTPVYKILGFVASTVFVGMAGAVYGYYLSFVDPLGMFNILLSVQIILSVLLGGRG
                     TLWGPVLGAFIVEPLNEFANNSLGGGNARLLVFGGLMALVVLFMPRGIVPAISEFLER
                     RRTRGRAGLVGERLGAPIELKEREVPTAPAAVSGQTLLEVKNVAKRFGGLRAVDSCGF
                     AVPEGSITALIGPNGSGKTTLFNLIDGTMPLDSGEVWFDGRRIDRQRPWNRAHAGVGR
                     TFQITRLFKEMTVLENVVAPIREFRLRQLNADAVSGPEAERAEELLEFVGMLKYRDVP
                     ASALSYGQQKLVELAQVLMLDPKLILLDEPAGGINPTLIERMGDMIRELNSRGKTFLI
                     VEHNMPFVLGLCDPVRVLGRGKLMAQGTPEEVQRDPRVLDAYLGDDFAVEPQVVEA"
     gene            24773..25522
                     /locus_tag="VF752_00475"
     CDS             24773..25522
                     /locus_tag="VF752_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854047.1"
                     /translation="MLSLKAVVAGYGGGDVLQGVDIEIAEGSVSCIVGPNGAGKSTVL
                     RAISGLLNPRLGEITLEGKRINGHAPSEILTMGIAQVPQSNGLFPNITVKENVLMGAY
                     LRRRDRAFLKRRYAELEEFFPVLTERGGDKAGNLSGGQRRMVEFARSLMMEPKIVMLD
                     EPSLGLDPKALKMVHESVNRMIERGRTVLMVEQNVRFGLRLADHGIVMESGRVLLDGK
                     ADDVLNNPEMAALYFGGSVKEPAPEAPPVRA"
     gene            25524..26402
                     /locus_tag="VF752_00480"
     CDS             25524..26402
                     /locus_tag="VF752_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8854048.1"
                     /translation="MTGALWAAASGIGFGLFQGLNRRAVRDIEDPYASTFIQLSIAAL
                     VLVSVTLLSGEQSAVRHASAWGVTAFALAGIVHFLGGWTFLNISQQRIGAARSSPLLT
                     VAPIFGVVIAAIVFQELPSALALLAMVPMVVGAYLVSSRSRGPEMDWTDALPGLATAL
                     MWGSSPVLTVEGLKTLHSPLAGVSIGMLAAVAAYGLALAWRGAGLRLATTSRGALNFK
                     VAAAVLVAIATWWRWLALDDASVGVVLALSLLSVPVVLFVAPLLVGRHLEHVTARVWV
                     GSALVVGGALALIAVG"
     gene            26407..27420
                     /locus_tag="VF752_00485"
     CDS             26407..27420
                     /locus_tag="VF752_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8854049.1"
                     /translation="MVTDIHAHVIVPEILRDAAPEEEWRPLVRWEHGVQVVEVAGRGV
                     RSMVEEVVDIEGIVAAQLEAGVDRVVLCPFVPLLFYDAEPEEGLRRCRIQNEGLARLV
                     RARRERVAALGAVPLQDPELAAYELRAVMAGGELSGVEIAASVRGLYLGDDRFEPFWA
                     AAEETRAVVFVHPTTRGFDASVFGEYYLWNAVGNPMETTVTAAHMVLAGVLERHPGLR
                     VVLAHGGGALPALRGRLRHAYEVQPPARARLRESPDESIKRFYFDTVVHDPTLLRALV
                     HYAGPEHVLLGSDYPFDMGDLRPADTVRACELNRDVEAAILSGNTERLLATVGARREE
                     IIR"
     gene            27417..29021
                     /locus_tag="VF752_00490"
     CDS             27417..29021
                     /locus_tag="VF752_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8854050.1"
                     /translation="MSETAHIVVAGGGHNSLVTAAYLAKAGHECLVLDARPIPGGGAA
                     TEELLIPGYKLDSCSTGHTLIQVNPLMLNDELGLKSDYGLEYLEPDPIAHVAFPDGQQ
                     LTHWLDLDRACEELARFSPRDADIYRRFMAEYDDVKDAFARARSAPIGFGPSLDELLA
                     EHPRGRIWMRRQAISAWDIIKREFESRHVQAYLLWQAFQTLVPVDAAGSGVNAYSIIF
                     GRQRRSWTIPRGGSGSLTDALVRCIEDHGGTVLCDRRVTRLILEDGRCVGVETDDGEQ
                     YLAGEAVLSSIHVKHLVDMAPADAWGEEFVYGVETYDIGMPGMAAYFATSEPPLFETP
                     DGSRSAVSAGTVGWPEEVVKLGRDIKDGRFVEDVRWLLAATPTIVDPSRAPEGMHTMK
                     LLSPQTWETPAGRSWEEMKDEVAQRQLEHLRRFAPNLTDDKIVARYVKSPADIERSNP
                     HMVHGAFHGGDRSYAFSGKLRPVPGWASHRMPIPGLYQTGATTSVGGSITGIPGRNAA
                     IVMLSDLGTSLEEVVAGAGRARAGSR"
     gene            28990..30045
                     /locus_tag="VF752_00495"
     CDS             28990..30045
                     /locus_tag="VF752_00495"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8854051.1"
                     /translation="MPDARVQAAVDHWGPRFMQAGVEYGDFVATMARIETWNEWLDEW
                     CVTAEMHAGLARDAEEAGRPRTAGEAWLRAAVGYHTAKFVWMLDHEKSREAADKAVDA
                     LRRAHAHLDPAAERLEAPLDGGRVVANLRRPPNGERPQLAVLIPGLDSTKEEFFNLEE
                     FFLNRGMATLSMDGPGQGECGYDVPIRPDYEVGLSAALEAVAGHDDLDLNRIGVVGVS
                     MGGYHAPRAATFEPRVKAVVGLSGPFCFGEIWDQLPQLTRETFTEKSRAASEEEARDK
                     ACELDLDGVIDELDKPALFVTGRLDRLIPWEQTAKQAERAPQGEFVCWEEGNHGCSNI
                     PYKARSLMADWMAEQLA"
     gene            30042..31016
                     /locus_tag="VF752_00500"
     CDS             30042..31016
                     /locus_tag="VF752_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8854052.1"
                     /translation="MTAVALVGTGMWGPRLAAAAERAGLRLVTCFSRDDERRGEFAER
                     FSCQAAASFEDAIGHPEVGGVILATPNDVHAEQALACAERGRHVFVEKPIADTTEAGE
                     RMREACETAGVTLMVGHAFRRLGAARRAKELVDEGALGRVVLAEANFSLPGKLGPQAW
                     RYYRDRNPGGPLMQLGIHHVDTLAHLLGPVRRSSGRFAHVHTQAEIDDVGVVVLEFQS
                     GALGSVTGSYVSPKTFDIRLFGTDAVIEYRTDMSVWPDAEKMDPATTLTLSGSRVEFD
                     ERDMLAEELAEFGRCIRGEAEPETGADEGIAALRVVLDAVASGAEVAA"
     gene            31016..31978
                     /locus_tag="VF752_00505"
     CDS             31016..31978
                     /locus_tag="VF752_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8854053.1"
                     /translation="MRAAIVRQAGTPPEVAERDAPAPGEGQTLVRVRAATLNPVDLHI
                     AGGRFFTGPPQFPYVPGGEGVGTLAGGNGKRVRFECWYVGYGGDGSLGELALAATESL
                     TELPDEIDDALAAALGTVGTTAWLALERAQLSEGETVLVLGATGAIGQVAVQAARLQS
                     AGRVIAAGRNTERLEHARELGADAVVELRDGMGRHELTAAFREAGSGGVDVVVDPLFG
                     EVAAAALEAGNPGARLVNIGRVAGTESVFTPATLRNKAAIGLSTHYSPVEEKKRVYRV
                     LVDLALKGKLTLEHEVLPLDEIGQAWSMQAGSPGRKLIIDPAAS"
     gene            31975..32439
                     /locus_tag="VF752_00510"
     CDS             31975..32439
                     /locus_tag="VF752_00510"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8854054.1"
                     /translation="MTLHDTEQHVVVNHASGEMVGPMATALHLRDFPTLVSDEPPSRG
                     GEDRGPTPLELLLASLCACTTVSTGRMAAKLRFEYSDLTTRAEGELDTRGRKGAADVP
                     VHYRTVRLRVRIATEESDARIARLADLVGRYCPVDSLMRAAVPDYEVTWERL"
     gene            32424..34661
                     /locus_tag="VF752_00515"
     CDS             32424..34661
                     /locus_tag="VF752_00515"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX8854055.1"
                     /translation="MGTAVDATRARLIGQPLPRREDERILRGRTSYLDDIELPELAHA
                     AFVRSPHAHARVNEIRAPAEAPGLLTVITAESLGERVRPFPVQAPPEMETADEPHPVL
                     AAGEIRYVGQAVAAVVAETRALAEDAADLVEVDYEPLEPVLDPRAGGPRLTHWTRSSG
                     DVEGSFERADHVVSTSHSIPRVVASPMETRGTVASWDSGSGMLTMWVSAQDPHRPLSQ
                     LAHSLRRPEDSIRVIVPDVGGAFGSKGVAAPESAALAIAAMDLGRPVKWAEDRLENFL
                     SAYQGRGMDVDVELALAADGRMLALRARIFADLGGYLLTTTAIPAHTTAMLMTGVYDI
                     GAAEVNVVGAQTHKVPTGPYRGAGRPEAAYALELTVDAAARELEMDPVELRRVNLISE
                     FPHETPLGFTYDSGEYARCLQRAAELAEFTEQDAGTGRRLGTGVGMYVERAGGMWESA
                     HVTVEPDGRVIVRSGSNPHGQGHDTVFAQIAAERLGVDIGDVALRFGDSAIVPRGVGS
                     FASRSVAMGGSAIVRAIDDIRDKCATIAAHLLEAPPEEIAWTSDGGLSAGDGRELSLR
                     EIAAAAYQPPRLPAGMEPGLEASARFASNQVFSSGAYAAAVAIDEATGRLEVLKLVAV
                     DDAGVIVNPLLADGQVIGGAAQGLGATLVEEAVYDENGQLRTGSYADYSLMTAVEMPH
                     IETDFVESPSPLNPLGAKGIGEGGAIGTPAAIGNAVVQALGGRIVHPPYTAEKLWHAM
                     QGEST"
     gene            34658..35251
                     /locus_tag="VF752_00520"
     CDS             34658..35251
                     /locus_tag="VF752_00520"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8854056.1"
                     /translation="MKLNNEFVVAAPLERTWSALLDVPRVARALPGATIDSEPADGAY
                     RGTMKVKFGAVTAEYAGTAMLQDVDEDERVASFRVEARERRGQGTAAATITSRLAEDS
                     AGTRVSVETDLAVTGRQAQLGRGIMEDVAGGVLERFADGLEQELAGGSPGPVQPPGSE
                     EALDLGGAVLGPLRERAAVFGAGVALGFLAGWLLRRR"
     gene            35248..36045
                     /locus_tag="VF752_00525"
     CDS             35248..36045
                     /locus_tag="VF752_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX8854057.1"
                     /translation="MKPAPFEYAAPRSVDEALGLLGEDSKALAGGQSLVPLLNFRFAR
                     PELLVDLNRVSELSYIRRSGGVLHIGALTRQATLERSGVVWQHWPLLAQAVRHAGHPQ
                     TRSRGTVGGSAVHADPTAELPVALTALDARFHLSSARGRRTLTAPEFFVATLISAVEP
                     DELLVEVEVPALPEGARTAFVEYQRTHGDFAVAGAAVVRGPGDGSAIALLGAGTTPVR
                     AHEAERALTAGAGAREAAELAAGALTDGWRRALTTELVREAIERAAA"
     gene            36042..36503
                     /locus_tag="VF752_00530"
     CDS             36042..36503
                     /locus_tag="VF752_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8854058.1"
                     /translation="MRIGVEINGTLYQGEVEPRTLLSDFIRHQAGLHGTRVGCEHGVC
                     GACTVQLDGEPIRSCLMLAVQANGRAVRTVEGLAPDGELQPLQRAFHEKHALQCGFCT
                     SGFLMSLEPFLRDHPEAGEDEIREALAGNLCRCTGYEAIVEAVKSAAEEMR"
     gene            36500..37573
                     /locus_tag="VF752_00535"
     CDS             36500..37573
                     /locus_tag="VF752_00535"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8854059.1"
                     /translation="MKQETDLEAMYRLFSWRITSNYVPHWDFAQMKAEISEWDEWCRV
                     WSRYASRHAGLGDAAAADGDERAAGAHYVRAGLFYHWASFVFPHRPDDFRAALAGMGE
                     CWRRAAPFTEPPMELVDVPFDGTLLPGYLRKPAGVERPPVAVLVPGADSTKEELFNLS
                     EHILERGVATFVFDGPGHGAVSFELKLRPDYEVPIGAVVDWLEQRDDLDTARLGVCGI
                     SYGGLFACRAAALDARVKAAVSMSSWYTPAGRFQNQDHISQVGLRQYMGEDPGAVQDA
                     MTLEGVADKITVPLLQVYGGRDPASPPEHAYRVEAEAQGPTTTVVYEDGVHVCNNLWY
                     EARPLVGHWLARALQPARSSPRS"
     gene            complement(37543..>37920)
                     /locus_tag="VF752_00540"
     CDS             complement(37543..>37920)
                     /locus_tag="VF752_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX8854060.1"
                     /translation="TGLYEELHEVADAAFDAIAGRLRAGATAAELVQASQVIEDAGFT
                     IRDDLVHGFVGGYLPPVLGSTSRALTEVPDFSFEAGMTVVVQPNVVTPDETAGVQTGE
                     LLLVTESGAESLHDYERGLLRAG"
BASE COUNT         5920 a        12911 c        13369 g         5720 t
ORIGIN      
        1 gggttcatct acacggtgtc gctcgcgggc acgacgggcg agcgcgcgga gttgccgccg
       61 ggcctggcgg agactgtgcg ccgcgtgcgc gccgtggcga ccgtgcccgt cgcggtcggg
      121 ttcggcatct cgacgggcga acaggcgcgg cgcgtggccg agctcgccga cggcgtgatc
      181 gtcggcagcc gcgtggtgcg agctgcgggc gagggcggcg ccggcgcagt gcgcgacgtc
      241 gtggccgagc tggccgcggg cctgaccgcc gactgacgcg ggtccgtgcg gatcgctagg
      301 ctgccgggcc gcatggcagt tctcgttcta ctcgtgctcg gactcgtgat ctgggtcgtg
      361 ggctggtcga tcaccgggaa cggcttcgac ttcttcatga tcacgctgct cctgctcgtg
      421 atcggcgcgg ccatccacat cgtcacgccg cacctccggc ggatgctggg gcacgagccg
      481 ccgccgctgg attagccgcg ggccggatta ccatggtcgt gccgtgagcc cgaccacgaa
      541 acgcgacccc tgggactctg atccgctctg gggtgatgcc cgcgaccgtg tgcgcggcga
      601 ggtgcaggcc gcgttcaagc cgaaggccca gacctgcccg tcgtgcgggc gtaccgagga
      661 gacggcggct cgcaactgcc cccactgcgg ctcctcatac gtgttcacgc ggcagcgcgg
      721 gctgtcaaag cgcgtgcggg ttgccctcgg cgcgtcgctg gcagtcctgg tcgcgggaat
      781 cgccgtcgcg ctgatcgtct tcgtgccgaa gatcggcgac acgaagcgcg agaacgcggc
      841 gcgcgatcgc gccgccgagg cggcgttcat caagcgtgag accgcgcggc tcaagcaaga
      901 tcaggcgcct caccgcgcga ggtcggaagc cgccgcgccg tcggccgtgg ttgcggacct
      961 ccagcgttcg atcttcgccg acgcgaaggc ccggctcgag gccggctcgt tcaaggtccc
     1021 ggtgaagacg gtgcagtgca cgcgcatcac gctcggcccc ttggcgacgg gaccccgcgg
     1081 cggctacgac tgcctggcgg tgacgagcta cgtcagctcg aacggcaaca accaggcggg
     1141 cgcgctcggc tacccgttct gggcggtggt cgacttcaag aggcgcacct acacctggtg
     1201 caaggtcaac ccgaagccgg gcgagcgcgg ggtgcagtcg aaggaaccga tcgtgccact
     1261 cgtgccggcc tgccagatca agccgaacga ttaggcgccg gccaggctcc tagccgaaga
     1321 acacctcggc cacctcgatc tcctcgggcg acaccgtgtg cagctcccgc acggcgtcct
     1381 cgagcggcac cagctcgatc ttcgtcgagc gcagcgccgg catcatcccc caggcgccgt
     1441 cggtcgccgc gtcaatcgcc gcgacgccga aacgtgtggc cagcacgcgg tcgaacgccg
     1501 tcggagtccc gccccgctgg atgtgcccga gcacggtggt acgcgtctcg aagccggtgc
     1561 ggttctcgat ctcatgctcg aggcgctgcc cgatcccgcc gaggcgcatg tgcccgaact
     1621 cgtcgagctc gccgccaagc tgctccatcg tcccttcgat cggcacggcc ccctcggcca
     1681 ccacgacgat cgcgaagtag cgcccgcgcg cgtgccgtcg gcggatgagg ccgcagacgc
     1741 gctcgatgtc gaacggcttc tccgggatca ggatcacgtc ggcgccaccg gccaggccgg
     1801 agtagagagc gatccagccg gcgtgccggc ccatcacctc gaccaccaga ttgcgatggt
     1861 gcgactcggc ggtcgtgtgc aggcgatcga tcgcctccgt cgccacctgc accgccgtgt
     1921 cgaatccgaa tgtcatgtcc gtcgcgccga ggtcgttgtc gatcgtcttc ggcacgccga
     1981 gcactggcag ccccgcggcg tgcagcttcg cggccgcgcc cagggtgtcc tcgccgccga
     2041 tcgcgatcag cccgtcgagg cgctcggcag tcaggttctc tctgatccgg tcaacaccgt
     2101 cgtcgcgctt gaacgggttg gtccgcgatg agccgagaat cgtgccgccg cgcggcagga
     2161 tcccgcgaac ggactcgatc gtgagctcct ccgcgtcgcc ctcgatgggc ccgcgccagc
     2221 cgtcgcggaa tccgacgacc tcgtggccgt agtggtcgat gcccttgcgg acgatcgcgc
     2281 ggatgaccgc gttgagaccg gggcaatcgc cgccccccgt gagcacgccc atgcgcacgc
     2341 cgcgcaacat accgactaga cgaagcccgc ttcacgaacc cccaccaaaa cccgctttgg
     2401 agagccgcat taagccagcg ttaggccgcg gtatggcgcg cttgtcattg gtccgcgctg
     2461 gggaatagga tgcgcgaccg ttcagcgcaa tggctggtca ccagaggagg gaagcaactt
     2521 gcgaggacgt ttcgcggtat ccggctgcct gctggtcgcc gccgcggtgg ggctcaccgc
     2581 gtgtggcggt ggaggaggcg gtgggggcgg aaccaaggct cagggcaaca cgctcacgat
     2641 ctactcgagc ctgccgctcc agggcgcatc ccgcccgcag tcagaggcac ttgtcaacgg
     2701 catgaagctg gccctcaagc aggccaacaa caaggccggc aagtggagca tcaactacaa
     2761 atcgcttgac gactcgacgg ctcaggccgg caagtgggat ccgggtcagg tgtcctcgaa
     2821 cgcccgcaag gcggctcagg acaagtccac gatcctgtat ctcggcgagt tcaactcggg
     2881 cgcgagcgcg atctcgatcc cgatcctgaa ccgagcgacc atcgggcaga tcagcccgtc
     2941 gaacacggcc gtcggcctca ccgccgacgt tccgggcgcc gagccgggcg agccggcgaa
     3001 gtactacccg acgggcaagc gcacgtatgt gcgcgtggtc ccgaaggaca cgatccaggg
     3061 tgcggcgctc gccgtggtca tgaaggacga gggctgcaag aagccctaca tcctcaatga
     3121 caaggaggtg tacggccagg gtctcggcaa gaacacccag aactcgctga agacggccgg
     3181 catccccgcg cttgccaacg agggctggga tccgaaggct ccgaactacc gctcggtcgc
     3241 ctcgaagatc gccggcaagg gtgcggactg catcctcgag tccggtatca cggacaacaa
     3301 cgccgtgcag ctcaccaagg acctcgcgtc cggcgtgccg aacgcgaaga tctacggccc
     3361 ggacggcgtg tgcgagtccg cgtgggcgga cccgaagaag ggcggcatcc cgacgagcgt
     3421 cgacagcaag gtcgagtgca ccgtcgcgac gctggatccg aacagctacc cgccggaggg
     3481 caagaagttc ttcgcggact acaaggccac ctacggcgag tcgaacccgg atccgtacgc
     3541 cgtctacggc tacgagacga tgtctctcgc cctggacgcg atcaagcgcg cggctgccaa
     3601 tggcaacgat cgccaggcgg tgatcaacgc cctgttcaac acgaaggacc ggaagagcgt
     3661 gctcggcacg tacagcatcg acaagaacgg tgacaccagc atcaccgact acgggctgta
     3721 caaggtcaag gacggccagc tgaatttcga ccgcgtcatc aaggccaagg gggcttccgc
     3781 ctaggcccga tcaggagcat gattgggggc ggacgggtct tcccccgtcc gcccttagct
     3841 ctgatagacc ttccgccagc ttggaagcca cctcagttcc agtacgggcc gcgcgcactg
     3901 cgccggtcgc cgccgtgcgc actttcctgt cgcgctacgg cctcatcttg gtgctcctga
     3961 ttctcccggt cgcgtacggg atccatgacc tccaggtgga cggcaacctc acgcgcctcg
     4021 gcgagaacct caaggacggg atctcgaatg gcgcgatctg ggcgctcgtc gcgatcggct
     4081 acacgctggt ctacggaatc atcgagctga tcaacttcgc ccacggcgat gtgttcatga
     4141 tcggctcgtt cgtcagcttc agcttctgga gcacgctcgg gctcacgctc acgtcaaaag
     4201 gcttcggcct gttcttcggg ctcctcatca cgctggtgat cgcgatgctc gcctgcggca
     4261 cgctcaacgt gctgatcgag cgggtggcgt acagaccgct gcgcaacgca cccaagctcg
     4321 cgccgctcat cacggcggtg ggcttctcct tcatcctgca gaacgtcggc ctcgagtggt
     4381 tcggcggctc gcagcgcggc gtcgacgacc tcatcaacgc acagaagacc ttcacgacca
     4441 tcggcggtgt gacgatcacg cacgcggacg tactcgccgt ggctgtcacc gcgccgctcg
     4501 tcgccctgct cgcgtggttc gtgacgagca cgcgcctcgg caaggccatg cgcgccacag
     4561 cgcaggagcc cgaggccgcg cgcctgatgg gcgtgaacgt ggacaccacg atctcgctca
     4621 ccttcctgct cggcggcatc atggccggcg ccgccggcct gatctacgcg ctttatcaga
     4681 ccacgatctg gttcttccag ggcttccagg ccggcctgat cgccttcacc gcggccgtca
     4741 tgggtggcat cggcaacatc cagggcgctg tgctcggcgg cctgatcatc ggctgcatcc
     4801 agcagatctc cgacaaccgc atcggcggcg agtggacgcc cgcgatcgta ttcgcctacc
     4861 tggtgctgat catggtgttc aggccgcagg ggctgctcgg cgaggagacg agggaggcgg
     4921 gatgagcgcg atcgcggcgg gcttgcgcga ctctcgcgag cggttcaacc agctgccgcg
     4981 ccggaccatc tgggcgatcg gcgccgtggt gctggtgctt ctgatcctcc tgcccttcgc
     5041 ctaccagccg gccagcgggt tcatcgatga ctgcgtcacc gcgctcgcgt acgtggtgat
     5101 ggcgctcggc ctcaacatcg tcgttggctt cgcgggcctg ctcgacctgg gctacgtcgc
     5161 gttctacgcc atcggcgcct acgtgatcgg ctggtacggg tcaggcttct tcgcctcgat
     5221 caacggcagc aagggcttcc acttcgtcgc cgagtcggcg agccacctgc ccggcatcca
     5281 catcaacttc ctcttcatcc tcgtaatcgc ggcactgttc gccgcgctcg ccggcaccgt
     5341 gatcggcctc ccgacgctgc ggctgcgcgg cgactacatc gcgatcgtca cgctcgcgtt
     5401 cggcgagatc acgcgcgtgt tcgcggtcaa cgggacggat atccacccgt tcggcaagaa
     5461 ctacccgctc acgctcggcc gccaggggat cacgccgatc gacccgatcg acctcccggg
     5521 cctgaaggcg ttcgatccgc tcaacctgcg cccctggtac tggttcggcc tcgcgctggt
     5581 gctgcttgtg ctgttcgtga acttccgcct gcgcgactcc cgcctcgggc gcgcctggat
     5641 cgcgctccgg gaggacgaag tggcggcggc gagcatgggc gtgccgctcg tgaagacgaa
     5701 gctgatggcg tacgcgaccg gcgctgcatt cggcggcatg gcgggcgcct tcctcgcgtc
     5761 ctatcagaac acggtgaacg cggaccagtt cgagttctcg ttctcgatct tcgtgctggc
     5821 gatggtgatc ctcggcggcc tcggttcgat ctggggcgtc gtgctaggcg cggtggcgct
     5881 ctcgttcatc aacagccgcc tgatccccga cgtgctcaac ggcgtcccgc acaagatcgg
     5941 cctgaacttc gacctcaccg agctgagctt cggaatcttc ggcttcctgc tcgtgatcat
     6001 gatggtgttg aaacccgagg gcctcctgcc cgaacggcga aggcagatcg agttcgaaga
     6061 aggcgtcggt gcggacgagg cgctctacga ggcgcgcgca tgagcgacgg cgccaccacc
     6121 tcgcagccgg accggaccgc accggcgggc gcgaacggcg gggatggcgg ctcggcgctg
     6181 atcctgcagt cggtcgccgt tgggaaggtg ttcggcggtc tggtggcggt cgacaacgtc
     6241 gatttcgaga tcccgcgtca ttcgatcgtg tcgatcatcg gcccgaacgg cgctgggaag
     6301 accaccttct tcaacatgct caccgggctc tacaagccga gctcgggccg gatcgcgttc
     6361 gaggacgggg acatcacggg catgcggccg gacaagatca ccgcgctcgg cgtggcgcgc
     6421 acgttccaga acatccgcct cttcgcgacg atgagcgggg tcgagaacgt gatggtcggc
     6481 cagcacgcgc gcatgcgcgc gggcctcttc gggtcgatcc tccgcctgcc ccgcgttcgg
     6541 agtgaggaag cgcgtgttga ggagcacgcg cgcgagatgc tcacctacgt gggcctgcgc
     6601 ccggggctgc acgaccagat ggccgcgaac ctctcctacg gcgatcagcg cagagtcgag
     6661 atcgcgcgcg cgctcgcctc ggatcccaag cttctgctgc tcgacgagcc gacggccggg
     6721 atgaacccga acgagtcggc gcgtttgacg gacttcatgg agacgctgcg cagcgatcgc
     6781 ggcctcacga tcctgctgat cgagcacgac atgaaggttg tcatgggcgt gtccgaccgc
     6841 atcaccgtgc tcgaccacgg cgagaagatc gcggagggcc cgccgcagga ggttcgcagc
     6901 gacaagcgcg tggtggaggc gtacctcggc aagcaggagg gcgagtagcc gtggcgatgc
     6961 tcgaggtcaa cgacatccac accttctacg ggaacatcga ggcgctgaag ggcatctcgc
     7021 tggaggtgaa cgagggcgag gtggtcacgc tgatcggctc gaatggcgcc ggcaagtcga
     7081 ccacgctgcg ctcgatttcg ggtctcagcc cgccgcgcga gggctcgatc cgcttcgagg
     7141 gcaacgagat cggcgagacg ccgccgcagg acatcgtgcg gctcggcatc tcgcaggcgc
     7201 ccgagggccg caagtgcttc ccgcgcatga gcgtgcgcga gaacctcgag ctcggcgcct
     7261 atctgcgccg cgatgccagg gtcagcgacg acatggatcg cgtgttcgac ctgttcccgc
     7321 gcctgaagga gcgcgagagg cagaaggcgg gcacgatgtc cggcggcgag cagcagatgc
     7381 tcgcgatcgg ccgggcgttg atggcgaacc ccaagctgct gctcctcgac gagccctcga
     7441 tggggctggc gcccgtcctg gtagatcgga tctacgagac gatcgcggag atcaacaagc
     7501 agggcaccac gatcctgctc gtcgaacaga acgcgaacta tgcgctcgac gtgtcgaatc
     7561 gcgcgtacgt gctcgagacg ggcgcgatcg cgctcgccga cgactccgcc tccctgcgcg
     7621 agaaccccga ggtgcagaag gcgtatctgg gcacatgacg ctcgtcttcg cactgctcgg
     7681 cgccaaggcg ctctacctca cctacgtctg gctgctgtcg accatcgccg cgtcgtggct
     7741 gtcggaccgc aagggctacg ggacgaaacc cggtctcgcc agcgggctgc tgctcacctt
     7801 cgtgggcacg ctgatctggc tcatcattcc cgcgcgcgcg agctcgccgt ggaaggtgca
     7861 ggggccgatc cccaagcgcg gcgggcagac cgtcgcgcag gcgcgcgcgg cgcagcacgc
     7921 gagcgagtcg gaccgccagt cctagcccac gagtatctcg ccgccgcggt cgggcacgac
     7981 cacgagcagg ctgcgccggg gctgctcggg ctcccggcgg actgggcgga acgtgaggtc
     8041 ggccgcgact tgcagcacct cgcgcagctc catctcggcg aacggcgcgc cggcgcagcg
     8101 tcgcaccccg ccgccgaacg ggatgaacga catcgggttc ggcggctcgc cctcgagcca
     8161 gcgctccggc cggaacgccg acgcctgcgg ccataggtcc tcgcggcgaa gcgcgagcca
     8221 gatgcaggcg gcgacctgca cgccggcggg cactcgccgt ccggcgatct ccactggttc
     8281 gagcagcagg cgcggggcga tggtgagcgc cgggcggatg cgcaggacct ctttgaccac
     8341 ggcgtcgagg taggccggat ctccctcgcg catccgcacg accacgtccg ggtggcgggc
     8401 gagccgctcg aacgcccacg ccagcgtggc cgcagaggtg tcgtggccgc ccatcaacag
     8461 cgcgaccaac tcgtcgcgca ggtggcgatc gctcggcggg ttgccgtcct cgtcgcgctg
     8521 ctcgagcagc atgtcgagga ctgaatcgcc gttcggctgc gcccgcctgc gtgcaaccgt
     8581 gtcgaccagc agcgcgtcga aacgctcgac agcgagccgg aaccgtcccc acggactgcg
     8641 tggcccgaag tcgcgctgca cgagcgccat cgccagcatc cgcggcagcg aacgcacatc
     8701 gtcgagcgtc gtttcgaccg cggtgcgaag ccgtgcaacc tcctcgtcgt cgcgcgcacc
     8761 gaatatcacg cgcaggatgg tctcgagtgc gaggtgtcgc gttcggtcga gcgtcgtgac
     8821 gcgcccgctc caggtgccga gctcgcggcg cgccagctcg ccgatcaagg gcgcgaacgc
     8881 gcgcatccgc tcaccgtgga acggaccctg caggaggcgg cgctcgcgca ggtgctcctc
     8941 gccgtcgagc acgaggatgg agtgtcggcc cgcgaagtcg cggaggaact cccagctctg
     9001 gcccgccggc gcgatgtcgg ggtcgaggcg aaagacgtcc cgcaccgcgt cggggtcgga
     9061 gaacagcacc atgggctgct ctgtccagta ggtgcggatc gtcgccgggt cgccgaaccg
     9121 ttcgtgcacg cgccgcagga acgcggcggg tcgcgccatc cacgcgatcg tctgccacgc
     9181 ggcgggagcg cgcggtccgg gaggcaggtc catgcgctca gcctacgcgg ggtcgctgaa
     9241 gcgaccggta cagtcggggc cgagtgttcg gcgtcgtcgc gctaaccgcg ctgctgctag
     9301 cagcgtcgca atcgtttcag tcctcgatcg agccgctccc ccgcccggtg caggcccagc
     9361 tcaagtccgc gcacgtgtgg cagaagggct gccccgtgcc gctgtcgggc ctccgcctgc
     9421 tcactgtgtc gtaccgcgat ttcaacgggc gcacgcagac cggccagctc gtcgtgaaca
     9481 aggcggccgc gcgcccgctc tcgcgtgtgt tccgcaagct ctaccggctg cacttccgaa
     9541 tccgtcacat gcagctcgac gacgtgtacg ggaagccgag cgagcgcccg cgcgacaacg
     9601 acgtgacggg ctccttcgag tgcaggcagg cggtgccgtc accctgtacc ggtgggcgcg
     9661 gcacgggctc atggtcgatg cacgcgtacg ggctggcggt ggacatcaac ccgcgcgaga
     9721 acccctacgt cggctgcggc cagagccacg accccgccgc gaggctctac agggaccgct
     9781 cgcggcaccg gcgcgggatg gttaccgcgg gcgtgatccg cgcattccgc tcgatcggct
     9841 ggggctgggg cggatcctgg agcggcaaca cgaaggacta catgcacttc tcgtcgacgg
     9901 gtcactaacc caccacgtcg cgcagcgccg gttcgagcgc ggggtggctg aactggtagc
     9961 ccagctcctc gagtcgcttc ggcacctcgc gcacgcccgt cgtgacgacc gatgccatct
    10021 cgccgtagag cgccttcagg gcgaacgcgg gcacaggggc aaacgcgggg cggtgcagcg
    10081 cccgcccgag cgccttcgac agctcgaggt tggtggcggg ttcgggcgcg gccaggttca
    10141 ccggaccgct cgcggcctcg ttgtcgagca ggaacagcat cgcgttcgcc acgtcgtcga
    10201 cgtggatcca cggcacgtac tgacgcccgc cggccacggg cccgcccagc ccggccttga
    10261 agaacggcag catccggtcg agcgctccgc ctttcggcga aagcaccacg ccggtgcgcg
    10321 tgcgcaccac gcgcacgcct agctcctcgg ctcgcgcggc ctcggcctcc cagtccacca
    10381 cgacgtcggc caggaagtcg ccgcgcgccg gcggcgcggt ctcgtcgacg cgctcgtccc
    10441 cgcgcggacc gtagtagccc gacgccgact gcgacacgag cacggacggc gtgggctgga
    10501 catcgcgcat gccggccacg aggttgcgcg tgccgagcac gcgcgagtcg cggatgcgcc
    10561 gctttgcgtc cgccgtccag cgctgggcga cgttctcgcc gagcagatgg atcaccccgt
    10621 cgcggtcggc gagcgcctgc gcgggcgccg gctgcgactc cggctcgcgc cagaccgccg
    10681 gctgcacccg ttcgccgaac acctcctcga gtcgcgactg gtcgcgcgtg aacgcggtca
    10741 cctcgtcgcc tcgttcgagc agcagctcca cgacgcgccg gccgatcatg ccggtcgcgc
    10801 cggtgacagc gatcctcaac ggagcctcct tatgccgtgg acgtgagcgg gtccgcgtgc
    10861 acgcgtgtgg cgccgcttcg ctccgcttcc ttggctacgg ccgtggccac gcacgagctc
    10921 acgaagtggt tgaacacgct cgggacgatg tagtcctcgg cgagctcgtc ctccccgacc
    10981 acgcgcgcta tgccctcggc ggcggcgatc ttcatctcct cggtgatccg gcgcgcgcgc
    11041 acgtccagcg cgccgcggaa gatgcccggg aaggcgagca cgttgttgat ctggttcggg
    11101 tagtccgagc gccccgtagc gacgatgcgc gcgtacttgg ccgcgagctc tggcggcacc
    11161 tccggcaccg ggttcgccat cgcgaacacg aacgggtcgg agttcatcgc gcccaggtcc
    11221 tcgggctgga tgatgcccgg ccccgacagc ccgatgaaga gatcggcgcc ccgcagcacc
    11281 tcggcaggga tgccgtcgcg gtggtcggcg ttcgtgttct cggcgagcca ctgcttcggg
    11341 gcgttcatgc tgccctcctc gtagtcgctg cggcccgagt ggatcgcgcc gaagcgatcg
    11401 cacgccacga tcgagcgaat gccgtagccg agcaggatct ttgccacggc cacgcccgcg
    11461 gcaccggcgc cgaccataac cgcgtcgatc tcggagatgc gccggccggt cacccggcag
    11521 gcgttgatca gcgcggcgag caccacgatc gcggtgccgt gctggtcgtc gtggaagacc
    11581 gggatgtcga gctcctcgat gaggcggttc tcgatctcga agcagcgcgg tgaggagatg
    11641 tcctcgaggt tgatgccgcc gaacgtgggc gcaatgagct tcaccgcccg cacgatctcg
    11701 tcgacctcct tcgtgtcgag gcagatcggc acggcgtcca cgccgccgaa ctccttgaag
    11761 agcatcgcct tgccctccat cacgggcatg gcggcctccg gaccgatgtc cccgaggccg
    11821 agcaccgcgg tgccgtcgct caccacggca acggtgttcg ccttgatcgt gaactcgaag
    11881 gcgcgctcgc ggtccccgtg gatcgccatg cacacgcgcc cgacgccggg cgtgtacgcc
    11941 atcgagaggt cgtcgcgcgt cttgatcggt gagttgagcc cggtgtggag cttgccgcca
    12001 cggtgcagct cgaaggtgcg gtcggtcacc ccgatcagtc gcgcgccctc gacggcctga
    12061 accgccgcca ccagccggtc cacgtgctct tggtcggtgc agaacacggt gatctcgcgt
    12121 accgccttgc gctccccggt ctcgacggtg tcgatcgccc cgatcgagcc tccggcttcg
    12181 ccgattgccg aggacagcag tcccagctgc cctggccggc ggtcgagctc gacgcggagc
    12241 gtcagcgaga actgggcgct cggcgtgact ggcatggtcg agaacttccc tccgggggag
    12301 tcgcttgaaa ggcgggccct gggtaacgcg cgtacgcgcg cccgcgaggc cggctcatct
    12361 tataggcggg aatttcgcag attgcgtgcg aaatcccgcc tggactggcg cccgcctcgc
    12421 ccacaactag gatgcagcgc gatgtcagac gaccgacgcg agctcttcct gatcgacggg
    12481 aacagcctcg cgtaccgggc gttcttcgcg ctcccggaga cgatcgccac gtcgaggggc
    12541 atgcccacga acgcgatctt cggtttcgcc tcgatgctgg tgaagatcct caccgactac
    12601 gggcccaagc cgaccatcgt ggcctgggac gcggggatgt cgggacgcga ggaggcgtat
    12661 gaggactaca aggccgagcg ccgcactcgg ccagatctgc tggccgagca gtggccgcat
    12721 ttgcagccgc tcgtcgacgc tttcggctac cgcaacgtga ggcaggaggg ctacgaggca
    12781 gacgacgtga tcgccacgct ggcgcgccgc gcccgggagg ccggcatcga ggtgatggtg
    12841 gtcacgggcg accgcgacat gttccagctg atcgagcccg gcatccgcgt gatggccacg
    12901 agccggggga tcacggacac gaagatctac gaccgcgagg atgtgatcga gcgctacggc
    12961 atcgcgcccg agctgatccc ggatttctac ggcctcaagg gcgacacctc cgacaacatc
    13021 ccgggcgtcc ccggcatcgg cgacaagacg gccgcgcagc tgctgcagga atacggcgac
    13081 ctcgagggcg tgctgtcgca catcgacgac atctccggcg cgaagcgcaa ggagaacctg
    13141 cgcaatcacg ctgacgacgc gcgcgtgtcg aaagtgctcg cgacggcgat cgacgacgtg
    13201 cccgttgacg tggacatcga acacctggtg gcccaggagc ccgaccgctc gacgctgcgc
    13261 agcaccttcc gcgagttcga gctgcgcgac ccgctccggc gcctcgagga ggcgctcggc
    13321 gagggggagg aggcggcacc gcgccaggtc gcggcggtgg agatcagcgc gcaggccttc
    13381 gaggttccgg ccgcggaggt ggcgcggctc gagggcgagc acgcggcgct ggccgcggaa
    13441 cgcccggagc gggaggacgg cgcggctggg cgcgcgctcg agttcgccgc ctacgcaggc
    13501 accgaggagg tgctcgtggg cgaggccgag acgctcggtg cgctcatgct cgcctggggc
    13561 ccgcggccgg tcgtggcgca cgactggaag tcgattgcga gtagcgaggc gccctgcgac
    13621 atgccgccgc tcgagcacga cacgatggtg gccgcgtatc tgatcgaccc cgcgcgccgc
    13681 aagtacccgc tcgacgagct ggccgacgac gagtcgatca gcgcgaacgt cgagggcgcg
    13741 gacggcctgg cggcgaacgc ggtgatcacg cgcgccctcg ccgagcgcca gctcgcggcg
    13801 atcgaggagc agggactgac caagctgcta cgcgaggtgg agctgccgct cgtgcgcgtg
    13861 ctcgtgaaca tggagcacac cggcgtgatg ctcgacacgc gccggctcgc ccagatcggc
    13921 gagacgttcg aggcgcgcat cgccgcgctc gagcgcgaga tctgggacct ggcgggcgag
    13981 gagttcacca tcggctcgcc gcagcagctc ggcgagatcc tgttcgagcg cctcggcctg
    14041 tcgcgcaagc gccgcggcaa gacgggcttc tcgaccgacg cgcgcgtgct cgcggcgatt
    14101 cgcgacgagc atccgatcat cgagaagatc gagcgctggc gtgagctgac caagctgaag
    14161 agcacctacc tcgacgcact gcccgagctc gtgtcgcccc aggacggccg cctgcacacc
    14221 acgttcaacc agacggcgac gaccacgggc cgcctctcga gcaccaaccc gaacctgcag
    14281 aacatcccga tccgcaccga gatcggccgc gagatccgcg cctgcttcgt ggcgccggcc
    14341 ggcgccaaac tgatctcggt cgactactcg caaatcgagc tgcgcgtgat cgcgcacgtc
    14401 gcggatgaag gcgtgctcaa ggagatcttc cgtcgcggcg aggacgtgca cgccgcgacc
    14461 gccggcgaga tgttcggagt gacccccgac caggtcgacc cgggcacgcg ctccaaggcg
    14521 aagatgatca acttcggcat cgtctacggg ctctcggcgt tcgggctctc cgaccggctg
    14581 aacatcccgc aggaggaggc tgcggagttc atcgagcgct acctcgcgcg cttcccgcag
    14641 gtgcagaagt tcatcaccga cacgatcacg agcgccacga acgacggata cgtgaagacg
    14701 ctgttcggcc ggatccggcg catcccggag ctgcgctcga acaaccgcaa cacgcgccag
    14761 ctcggcgagc gacttgctgt cagcacggtg atccagggaa cggcggccga catcatcaag
    14821 gtggccatgg tgcgctgcaa cgaggcgctc gcccacgagg ggctgaagac gcgcctcgtg
    14881 ctgcagattc acgacgagct gctgttcgag gcgcccgacg acgaggtgga gcgtgcgtgc
    14941 gcgatcgtcg agcgggagat ggtgagcgcc ttccctctcg agccgccgct cgctgtcgac
    15001 accggcgttg ggcagaactg gctcgaggcc aagtagctca gcggatcgtc acgagagcgg
    15061 tcccggcgag cagcagagcg aatcccgcca ttcgcaatgg ggtgaccccg atcttgtcga
    15121 ggcctaggat cccgagccgg tcgagcactg cggaaacgat caactgcgcc gataccgtgg
    15181 cagccaccac gccgccggcg ccgagcgtgc gcacggtcac gagcgacact gacacgagcg
    15241 ccgcgccgag caggccgccg gtcatgtacc cgagcggagt gttggtcagc ccacgcagct
    15301 cgccgtagcc accggcgacc gcgagcagga aggccatcac caccgcggag atcgccgtgc
    15361 tcacgaacgc cgcgccgagc acgctcgtgt gcttggcgag ctgcgagttc acgggcggtt
    15421 ggaaggcgat cagaccgccg acacacgccg tcgccagcac tgcgagtact cgttccatcg
    15481 gtggccacct ccggcgtcaa ggtcgagaag cggcgcaagc ctagaggctc gcggcggctc
    15541 atggccggac gacgacgcaa tcgtttgcag cggcgcgagg gcagcgctac gcttcgaccc
    15601 gatggcgagg atccgtcctg tgagcgaggc cgatgcaatt cgcgcgcgcg tgccggacgc
    15661 gctgcgcgcg tatggagaga cgcgagcgac ggtgctcgag gcgggcgtcg tggaccagcg
    15721 cctgaaggag ctgtgcgcgc gctacatcgc cgaggacgac gaggtcgtca actactcgac
    15781 gtcggacgcc ttcgacgagc gcgatcgcgc ggcgctcgac tgggtgcacg cgatcgcgtg
    15841 gaactcggac atggccgacg ccgatctctg gaaccggctc cgggccaact tcagcgagcc
    15901 cgagctcgtc gagctcggct acttcatcgc cttcacgctc gggcagtcgc actggctgcg
    15961 cacgctcggc ctcgagaaga gcgaaacgat ctgacgcaga cacaacggga ggggaacttg
    16021 ccgaacatca gctattaccc cgtggagaag ctcgaggacc cggagctcag ggcctacctg
    16081 gagcacgcgg cggcgcacgg gaccccgcgt cccgagagcc aggcgatccg ggcgcacgtc
    16141 ccggcggtgt tgcactcgtt ctcgaagaac tgggacgcag tgttccgcaa gggcgtgctc
    16201 gaccacccga ccaaggagct gtgtcgcgtc tacgtctcga agacgatcga ctgccattac
    16261 tgaggctctc aacggtctct cccgggtgga gagcaagggt taactgaaga tcactacgac
    16321 gagctcctcg agttcgagca ctccgatcgc ttcgacgagc ggcaaaaggc ggcactgctc
    16381 tacacgagcg cgatcgtgtg gaatcccgag ggcgctgacg acgcgctgtg ggagcggctg
    16441 cggctgcatt tcagcgagga ggagctcgtc gagctcggct acttcatcgc gatcacctac
    16501 ggacagcagc gctggatcaa gacgctcggg atcggccacg gcgaggtgct cgacctgacg
    16561 aaggccggtc tcgcaccgca gggtgagcag gcgcgctagg tccagcgggc cacggcgagc
    16621 tcgagcggga agtcgtcgac acactcgttg ccgtcctcgc gcacgatgaa cgtgtggcca
    16681 acgaacatgc caagcatgcc gtcgggggtg atcgggttcg gctcggccat cacgaccatc
    16741 ccggggcgca tcacctcctc gaacggctcg gcatcgattc gctcagcgtg cacgtgcggg
    16801 ccgtccgaca cgaagtcgat cccgtgagcg tggagcgggc gcgactgaac gccgttgtcc
    16861 cggaagaacc tgcccgcctc gcgcagtgcc tcgatcggct tgcccgggac gatctcggcc
    16921 accatgcgct cgtagcccgg cttcgcgacc tcctcccaga agcccctgat ctcgacagtc
    16981 ggctcgccca cgcacacggg tgagccgatc tgcgcgctgt acccgcggta tccggcggcc
    17041 agctccatga tgatgatgtc gccctccgaa agcactcgct tcgagggacg cggattgccg
    17101 aacaccatgg ccgggtcgga catcggcgtg gacccaatga tgaggaagtc gatgtcgccg
    17161 ccaccctcca ggatggcgct gcccgctgcc gcgcgaagct cgtactcagc cactcccggc
    17221 gcggcccgcg cgacgatcgc ctccatggcg tgctgcacca gccgccccgc cgcgcgcacg
    17281 cagtcgagct cctcctcgct gtgcacggac agcagctcgt gcatgaggcg ctcggtgaac
    17341 acgagctccg cgccgggcag cagcctgcgc aggacgtcgt actggttcac cggcaggtag
    17401 tcgccgtgac ggggatccac ctcgagaagg ccgatgcgcg tgcgctccag cccgagctcg
    17461 tggatccgct ccgcgatcac ctccgcgaag cgcccgccgc ggctcgcgcg cacgtccgac
    17521 agcgcgcctg cggtctcgcg cctcacagcc tcgatgtggg tgccgcccat tgcgtatacg
    17581 agcgtcggct ctccgtcgat ggggaagacc acgtacgccg cgagcgcatg ccactcccag
    17641 tggccgctca gccagagcat cccgccaccg aagctccagt ggctcggtcc gcccggcagg
    17701 atcacgcagt cgagctcgcg ctcgtgcatc ttctgccgga gcgccgcgtg gcgccggctg
    17761 tattcggtct ccgagaactg ctcgtacacc gcgtcgcggt aatacggcgt gtcgcgcatc
    17821 gtgttgaacg cgcggctctc atcgagacgg tggcgcagct cggaccaggg cagcgtcatg
    17881 gatctcctct cttcctcgtg cgggtgacgc ggttgctcgc ccgcagcgcg gcttccgcgg
    17941 cgagcatgtg ccgcgtctcc ggctcgcccg ggccgacgtg gaatcccttc gcacgacgct
    18001 ccacgtccaa gtcggcgacc gtcattcgcc ggtgctgacg cagatgctcg ctccaggagt
    18061 ccacgatgaa ggtctcgagc cagcaggcgc ggtcctcgag ctcctggtag agcctccact
    18121 gccgggcgcc atcgcgccgc cggatccgac cgagctcttc catagccgcc gcgaacgcca
    18181 gcacgtcgtc ctccctcacg cggtactcga gcgtgaccag caccgggccg tcaccggccg
    18241 ccggctcgat cggaagcacg ggctccgtcc aggcggtggc gggcgtcagg tcgggctcct
    18301 cgataccgat gagcgaggcg cgtccccggg ccgccaggct cacaaggagc accacggcag
    18361 ccgacaggaa cgcggcctcg acgctgaagc cgtctgcgac gagcccccac acgagcgctc
    18421 cggcagcgag gccgccctgc atcgagaact ggaacgccgc gagcgcgcgt ccccgaaccc
    18481 agtcgggggc ggccagctgg gctgcggtgt tgagtgtcga gagcaccgac agccacgcgg
    18541 ctcccgccgc gaacgtggcc acgcccgcca gcacgggctc atgcacgaat gccagcacgg
    18601 ccacggcggc ggccagcccg acggtcccaa acgacaccgc caggtcgagc gtggtccgcc
    18661 ggcgcagccg aggcagaagg gtggcaccgg cgatggcgcc cgcaccggcg catcccacca
    18721 gcacgcccag cccgctcgcg ccgagctcga gcggcccgaa cgcaacgaca ggaaggagtg
    18781 cccagaaggc gctcgctccc gctacgaaca ccagcgctcg caccagcacg gcgcgcacgg
    18841 gcggcgagaa gcgcacgaag cgcgcgccgg cgcgcatggc ttcgaacacc tgctcaggcg
    18901 cttcgtcggc ggccggccca ggcgagcgca gcgagagcac cagcgcgatg atcgccgcca
    18961 cgccggccgc ctccagcgcg aagagggcac ctgcgctgac caccgctatg accacgccgg
    19021 cgagcgcggg gccgacggcg cgggcgaggt tgatggccac gctgttgagc gctactcccg
    19081 cggccagacg accggggccc gcgagctcgg gcgtgagcga ctggaaggtc ggaatgctcg
    19141 ccgccatgcc ggcgccgagc gagaaggtga ggagcaggat cgataccggt gtggccgctc
    19201 ccgcgagcgt cagcgttgcc gccgtggcgg tggagatcag catccaggac tgcgttgcca
    19261 ggagcagccg gcggcggtcc acgatgtcgg tgaccgcgcc cgcgaggaac gcaagtagca
    19321 ctgcggggag gctggtggcc gtctgaacca gcgccacgag ggtcggcgag ccgcccagag
    19381 tggcgatcac cgtagcggct cccacggtgt tcatccagac cacgagctgt gacccgaaga
    19441 aggccgacca cagcgaacgg aactgtcgct cgcggagcgg ggcccacggg ccgctttgcg
    19501 atgactctgg cggcgatgtg gcggtggtcg tgttgttcat gtgggaggcg actgcaaacg
    19561 catgcataat gctccctgtg acggcccgca atgcgaaaga gcagttgcgg gtcactctgc
    19621 gcgacgtggc ccggctggcc ggcgtgcatc ccggcacggc gtcgcgagcg ctcaacgagc
    19681 agactcgttc gctcgtcaac gcggagacgg cgcagcgcgt gctcgccgcg gccgacgagc
    19741 tgggctacca cccgaacccg atcgcccgcg ggctgaagac caaccgctcg tacaccgtcg
    19801 gcgtcctcat cccggacatc gagaacccgc tcttcccgcc gatcatccgc ggcatcgagg
    19861 atcgcctcga ccaggccggg tatacgccgg tgatcgcgaa cacggagaac gacgccgagc
    19921 gcgagcggat cgacttccgc gccatgcgcg cccgccaggt cgacggcttc atcacggcca
    19981 cggcgcggct ggatcacgag ctcctcgacg aggtggcggg cgtgggcgtc ccgttggtgc
    20041 tggtgaaccg gcgggtcgaa gacgagtcac tgccgtcggc cacggccgac gatcgcgcgg
    20101 gcgtccggat ggcggtcgac ctcctggccg atctcgggca cgagcacatc gcgttcctcg
    20161 gcgggcccac cgagctgtct accgggcttc agcgcaagga gggcttcatc gctgggatgc
    20221 aagaccgcgg gctgaagccc gaccccgcgc ttgtcgcgac cggcgagcgc ttcacgctcg
    20281 acgagggaca aaggctctgc cacgaggcga tcgagagcgg caggcagttc accgcgatcg
    20341 tcgcgggcaa cgacctgatg gcgctcgggt gctacgacgc gctcgccgag cacgggctcg
    20401 catgccccgg cgacgtgtcg gtggtcggct acaacgacat gccgttctcg gaccacttca
    20461 acccgccgat gacgaccatc cgcatcccgc actacgagat cggccggtcg gcggccgacc
    20521 tgctgctccg tctgctcgag caaggccccg atgggagtac tcagctggtg ctcgaaccca
    20581 cgctggtggt gcgggggtcg accgctgcgc ttgacaagaa gtgacccctg cgattagctt
    20641 tgcacacgtt tgccgcaaac gtttgcacaa gcgcgtttga cgtgagggag cttcccacgc
    20701 ggttcgccgc ggggaccgat ccgggggaag agggatgaca agaatgaggt tccgtctttt
    20761 ggccctcgtg gccgtgtcgg cgctgatcgg cgtgggttgc gggggcggtg gcggcagcag
    20821 cgacaaggga accatcaagg taggcgcgtc gctgccgctg accggcgact tctcggagcc
    20881 gggcaaggcc gccaagcagg gctaccaggt ctggcaggcg atcgtcaacg agcagggcgg
    20941 cctagtcgac ggccgcaagg tccagatggt gatcaaggac gacgccagca accagaacac
    21001 ggtcgtcgcg gactacaacg cgctgatcag caaggacaag gtccagatgc tgctgggcac
    21061 gttctcgtcg ctgctcaacc tgccggcgtc ggcggtggcc gagcgcaacc gcatgctgta
    21121 cgtggagcct gcaggcgggt cgccggagat gttcagccgc gggttcaagt acctgttctt
    21181 cgcgcagcag gcgaccgccg atcaccaggg cgacgtcttc tccaattacg tcaagggcct
    21241 gccgccggat cagcggccga agaccgcggc gtatccgacg ctggacgatc ccttcgccca
    21301 gccgacctcg caggggatcg agaagatcct caaggccgca gggatcaaga ccgtctatcg
    21361 caagacgtac acgatcgacc agaagaactt cgactcgatc gcgaattccg tgaaggcgag
    21421 cggagccgat ctcgtggtca acggcgcgac gttcgaggac ggcgtgggct tcgtccgcgc
    21481 gctcctcaag atcggataca cgccgaagat gctctaccag accacggctc catcgctcgg
    21541 cgaccagtac gcgaaggcgc tcggcaaggg caacaccgaa gggatcttct acgcggtgag
    21601 ccacagcgcg aaggcgaaga cgccgggcaa cgagcagttc gtcgccaagt acaagcagat
    21661 gttcggcggc accgaggtgc ccgaggacgc ggccgacgcc tacgccacgg cgcaggtgat
    21721 gcaggccgcg gtcaaggcgg tcggcggcat cgacgaccag gcgaagctcg cggactggct
    21781 tcgcaacaac aaggtggaca ccatcctcgg cccgctcgag tgggacgaag cgggccggcc
    21841 gcagggccag ttcctcgttg gccagtggca gaacggcgcg ccggagatca tcctgccgaa
    21901 gggggcgacc gccaccaccc agtcgatagt gcccgagtgg aagccgagcg gcgcggggac
    21961 ttaagggggt gaggcagcgc ggggcgccac cgcgccccgc gccaacgcct cgcacacgcg
    22021 atgacggagt tcgtacagac actcatcctt ggactgctcg tagggggcgt ctacgcgctg
    22081 ttggcgtccg ggctcacgct gatcttcggc gtgatgaacg tcatcaacat cgcccatggc
    22141 gcgttcctga tcctcgcggc gttcatcacc tactcgatct ggcgcgccat cgggctcgac
    22201 ccgctggctg gcatcctgat cacgaccccg gtgatgtttg tcttcggctg ggtcgtctac
    22261 aacctcacgc tgcggcgcat acgcacgggg ccagcggccg ccaccgtgct tctcacgttc
    22321 ggcctcgcgc tcgtggtcga gggcctcatg ggcatcatct ggggcaacac cgcccacgcg
    22381 gtcacggcgc cgtacttcaa cgagtcattc cacatcggat cgctgttctt gcccaaggcg
    22441 caggtgtacg ggggtgtgct cgcgctcgtg gtgctcgccg tgctctggat cgtgctgact
    22501 cagtcgtggc tcggccgcgc gatccgggca gccgcggtca atccgcaggg agcggcgctc
    22561 gtgggcgtga acgtgtccgc cgtctccgcg gccacgttcg cggtcggcgt cgcgagtgca
    22621 ggtgcgggcg ggtcgatcgc cagcgtgctc tacaccttcc tgccgggttc gcactacgtg
    22681 tggatctcgc gcttgctcgg catcatcgtg ctcggcggga tggggagcct gccgggcgcc
    22741 ttgatcggcg ccctgacact cggcatcgcg gagacgatga ccgtcacgta catctccccg
    22801 tcgtgggcga ccgcggtgcc gtacgcgctc gtgttcgtgg tgttgctcgt tcggccctac
    22861 ggcatcctcg gcactcggct ccgcgaggac gtggtcactt gacgcgcgcg aagatgctct
    22921 ccgcgcccgc ggcggcgtgg agctcgtgga cgccgcccga atgggtcagc tcgcgccggc
    22981 tgcgctacgg gctgatcggc gccgccgccc tcttcatcgc cgcgtacccg ctgctctccg
    23041 acgaccgcta ctaccagaac atgatcatcc tgtcgctggt cttcgcgatc ggcgcgacgg
    23101 ggctgaacat catcacgggc tacggcgggt acgtctcgct gggccagggc gcgttcatcg
    23161 gcctcggcgg ttacaccgcg gccatcctgt ccacgaaggt cggcggcaac ccgttcgtct
    23221 gggtgccggt tgccggcgtg gtcgcgggtc tgttcgcggc cgcgctcggc gtgatcgcca
    23281 tgcgcgcccg cggtcacacg ttcgtgatcc tcacgatcgt ctttctcttc ctactccaga
    23341 tcgtcgcgat caactggcgc gagctgacaa acggcacggc cggtctcacg atgccacttc
    23401 cgcgctggag tcccgacatc gcgaactggc cgttctacta cgcactgctc gggctcctcg
    23461 tggtctcgat agcgatgtcc tggtggatcc ggaggacaaa gttcgggatg ggtctcgtgt
    23521 cgatccgcga ggacgaggac aaggccgaga ccgtcggtat caacacgccc gtctacaaga
    23581 tcctcggatt cgtggcgagc acggtcttcg tgggcatggc cggagccgtg tacggctact
    23641 acctctcgtt cgtcgatccg ctcgggatgt tcaacatcct gctgagcgtc cagatcattc
    23701 tgtccgtgct gctcggcggg cgtgggacgc tctggggacc tgtactcggc gccttcatcg
    23761 tcgagccgct gaacgagttc gcgaacaact cgctcggcgg gggcaacgcg cggctgctcg
    23821 tcttcggcgg gctgatggcg ctcgtcgtgc tcttcatgcc gcgcggcatt gtgcccgcca
    23881 tcagcgagtt cctcgagcgc aggcgcacgc gtggcagggc gggattggtc ggcgagcgcc
    23941 tcggcgcccc gatcgagctg aaagagcgcg aggtgccgac cgcgccggct gccgtctcgg
    24001 gccagacgct gctggaagtc aagaacgtgg cgaagcgctt cggcggtctg cgcgcggtcg
    24061 acagctgcgg cttcgccgtg ccggagggct cgatcaccgc gctgatcggc ccgaacggct
    24121 cgggcaagac gacgctcttc aacctgatcg acggcacgat gccgctcgac tcgggcgagg
    24181 tctggttcga cggacgccgg atcgaccgcc agcgcccgtg gaaccgcgcg cacgcgggcg
    24241 tcggccggac gttccagatc acgcgcctgt tcaaagagat gaccgtgctc gagaacgtgg
    24301 tcgcgccgat ccgtgagttc cgcctgcgac agctgaacgc ggacgccgtg agcgggccgg
    24361 aggcggagcg cgcggaggag ctgctcgagt tcgtgggcat gctcaagtac cgcgacgtgc
    24421 ctgcttccgc tctctcctac ggccagcaga agctcgtcga gctcgcgcag gtgctgatgc
    24481 tcgacccgaa gctgatcctc ctcgacgagc cggccggcgg catcaacccc acgctcatcg
    24541 agcggatggg ggacatgatt cgcgagttga acagccgggg gaagacgttc ttgatcgtcg
    24601 agcacaacat gccgttcgtc ctcgggctgt gcgaccccgt gcgggtgctg gggcgcggca
    24661 agctgatggc gcagggcacg cccgaggagg tccagaggga cccgcgggtg ctcgacgcct
    24721 acctcggcga cgacttcgcg gtggagccac aagtcgtgga ggcgtgacga agatgctcag
    24781 tctcaaggcg gtggttgcgg gctacggcgg cggcgacgtc ctccagggag tggacatcga
    24841 gatcgcggag ggctcggtgt cctgcatcgt cgggccgaac ggcgccggca agtccacggt
    24901 gctgcgcgcg atcagcgggc tgctcaaccc acggcttggc gagatcacgc tcgaaggcaa
    24961 gcgcatcaac ggccacgcac cgtcggagat cctcacgatg ggcattgcgc aggtgccgca
    25021 atcgaacggg ctcttcccga acatcaccgt gaaggagaac gtgctgatgg gcgcgtatct
    25081 ccgccggcgc gaccgagcct tcctcaagcg ccgctacgcg gagctcgagg agttcttccc
    25141 ggtgctcacg gaacgcgggg gcgacaaggc gggcaacctc tctggcggac agcggcgcat
    25201 ggtcgagttc gcgcgctcgc tgatgatgga gccgaagatc gtcatgctcg acgaaccctc
    25261 gctcgggctc gacccgaagg cgctcaagat ggtgcacgag agcgtcaatc gcatgatcga
    25321 gcgcggcagg accgttctga tggtcgagca gaacgtgcgt ttcgggctgc ggctcgccga
    25381 tcacgggatc gtgatggaga gcggccgagt gctgctcgac gggaaggcgg acgacgtcct
    25441 caacaacccg gagatggcgg ctctgtactt cggcggctcc gtgaaggagc cggcgcccga
    25501 ggcgccgccg gtccgggcat gacgtgacgg gggcgctgtg ggcggcggcc tccggaatcg
    25561 ggttcggcct cttccagggg ctgaaccggc gtgcggtccg cgacatcgag gatccgtatg
    25621 cctcgacctt catccagctt tcgatagcgg cgctcgtgct cgtgtccgtc acgcttctgt
    25681 cgggcgagca gtccgccgtc cgtcacgcca gtgcctgggg cgtgaccgcc tttgcgcttg
    25741 cgggcatcgt gcacttcctc ggcggctgga ccttcctcaa catcagccag cagcgcatag
    25801 gcgcggctcg cagctcgcct ctgctcaccg tggctccgat cttcggcgtt gtcatcgccg
    25861 cgatcgtgtt tcaggaactg ccgagcgcgc tcgcgctgct ggccatggtg ccgatggtgg
    25921 tgggcgcgta cctcgtgtcg tctcgctcgc gcggccccga gatggactgg accgacgccc
    25981 tgccagggct cgcgacagcg ctgatgtggg gcagcagccc cgtgctcacg gtggaggggc
    26041 tgaagacgct ccactcgccg ctggcgggcg tttcgatcgg catgctcgcg gccgtggcgg
    26101 cgtacgggct ggctctcgcc tggcggggtg ccgggctgcg gctggcgacg acgagccgcg
    26161 gcgcgctcaa tttcaaggtc gcggccgcgg tgctcgtcgc gatcgcgacc tggtggcgct
    26221 ggctggcgct cgacgacgca tcggtggggg tggtcctggc gctcagcctg ctgtccgtgc
    26281 cggtcgtgct gttcgtggcg ccgctgctcg tcgggcggca tctggagcac gtcaccgcgc
    26341 gcgtgtgggt gggctccgcg ctcgtagtgg gcggcgcgct ggcattgatc gcggtggggt
    26401 gagccgatgg tgaccgacat ccacgcccat gtgatcgtgc cggagatcct gcgcgatgct
    26461 gcccccgagg aggagtggcg gccgctcgtg cgctgggagc acggcgttca ggtcgtcgag
    26521 gtcgccggcc gtggcgtgcg ctcgatggtc gaggaggtgg tcgacatcga gggcatcgtg
    26581 gccgcgcagc tcgaggccgg cgtcgatcgc gtcgtgctct gcccattcgt gccgctcctc
    26641 ttctacgacg ccgagccgga ggagggccta cggcgctgcc gcatacagaa cgagggcctg
    26701 gcccggcttg tgcgcgcccg gcgcgagcgt gtggcagcgc tcggagctgt gccgttgcag
    26761 gacccggagc tggccgcgta cgagctgcgc gccgtcatgg ccgggggcga gctcagcggc
    26821 gtggagattg ccgcgagcgt gcgcggtctc tacctcggcg acgatcgctt cgagcccttc
    26881 tgggcagccg ccgaggagac gcgggcggtc gtgttcgtgc atccgaccac ccgcgggttc
    26941 gacgcatcgg tcttcggcga gtactacctg tggaacgcgg tcgggaaccc gatggagacg
    27001 accgtgaccg ctgcgcacat ggtgctggcc ggcgtgctcg agcgtcatcc cggcctgcgc
    27061 gtggtcctcg ctcacggcgg cggcgcgctg ccggcgcttc gcgggcgcct ccgccacgcg
    27121 tacgaggtgc agcctccggc gcgcgcccgg ctgcgcgaat cgcccgacga atcgatcaag
    27181 cgcttctact tcgacaccgt cgttcacgac cccacgctgc tgcgcgccct tgttcattac
    27241 gcggggcccg agcacgtgct gctcggatcc gactatccgt tcgacatggg cgacctgcgc
    27301 ccggccgaca ccgtgcgcgc gtgcgagctc aatcgggacg tggaggccgc gatcctgagc
    27361 ggcaacaccg agcggctgct cgcaaccgtc ggcgcgcgga gagaggagat catccgatga
    27421 gcgagaccgc gcacatcgtc gtcgcgggcg gtggccacaa cagcctggtc acagctgcct
    27481 acctggcaaa ggccggtcac gagtgcctgg tgctcgacgc gcgaccgatc cccggcgggg
    27541 gcgccgccac ggaggagctc ctgattcccg gctacaagct cgactcctgc tcgaccggcc
    27601 acaccctgat ccaggtgaat ccgctgatgt tgaacgacga gctcggcctg aagtcggact
    27661 acgggctcga gtacctcgag cccgacccaa tcgcccacgt ggcgttcccc gacggccagc
    27721 agctcacgca ctggctcgac ctggaccggg cctgcgagga gctggcgcgc ttctccccgc
    27781 gcgacgcgga catctatcgc cgcttcatgg cggagtacga cgacgtgaag gacgcattcg
    27841 cccgcgcccg ctccgcgccg atcggattcg gtccctcgct cgacgagctg ctcgcggagc
    27901 atccgcgcgg acgcatctgg atgcggcgcc aggccatcag cgcctgggac atcatcaagc
    27961 gggagttcga gagccggcac gtgcaggcgt acctgctctg gcaggcgttc cagaccctcg
    28021 tgccagtcga cgccgccggc tccggcgtga acgcctactc gatcatcttc ggccgccagc
    28081 gccgcagctg gaccatcccg cgcggcggct cggggtcgct caccgacgcg ctcgttcgct
    28141 gtatcgagga ccacggcggc accgtgctct gcgaccgccg cgttactcgg ctgatcctcg
    28201 aggacgggcg ctgtgttgga gtggagaccg acgacggcga gcagtacctc gcgggggagg
    28261 cggtgctctc gagcatccac gtgaagcacc tcgtggacat ggcgccggcc gacgcctggg
    28321 gcgaggagtt cgtgtacggc gtcgagacct acgacatcgg catgcccggc atggcggcct
    28381 acttcgccac gagcgaaccg ccgctcttcg agacgcccga cgggtcccgc agcgccgtgt
    28441 cggccggaac ggtgggctgg ccggaagagg tggtgaagct cggccgcgac atcaaggacg
    28501 gccggttcgt ggaggacgtc cgctggctgc tggcagcgac gccgacaatc gtcgatccat
    28561 cacgcgcgcc cgaaggcatg cacacgatga agctgctcag cccccagacc tgggaaacgc
    28621 ctgccgggcg gagctgggaa gaaatgaagg acgaggtcgc tcagcggcag ctcgagcacc
    28681 tccgccgctt cgcgcccaat ctcacggacg acaagatcgt cgcgcggtat gtgaagagcc
    28741 cagcggacat cgagcggtcg aacccgcaca tggtccacgg cgccttccac ggcggcgacc
    28801 gcagctacgc attcagcggc aagctccggc cggttccagg atgggcgtcg caccgaatgc
    28861 ccatcccggg cctctaccag accggggcga ccaccagcgt gggcgggtcg atcactggaa
    28921 taccggggcg caacgccgcg atcgtgatgc tcagcgacct cgggacgagc ctggaggagg
    28981 tggtggccgg tgccggacgc gcgcgtgcag gcagccgttg accactgggg tccgcgcttc
    29041 atgcaagccg gcgtggagta cggcgacttc gtggcgacga tggcgcgcat cgagacctgg
    29101 aacgaatggc tcgatgagtg gtgcgttact gcggagatgc acgccgggct ggcgcgcgac
    29161 gccgaggagg ccgggcgccc acgcacggcc ggcgaggcgt ggttgcgtgc ggccgtcggc
    29221 taccacaccg cgaagttcgt gtggatgctc gaccatgaga agagccgtga ggctgccgac
    29281 aaagcggtcg acgccctgcg gcgggcgcac gcgcacctcg acccggcggc cgagcgcctc
    29341 gaggcgccgc tcgacggcgg gcgggtggtg gcgaacctca ggcgcccgcc caacggcgag
    29401 cggccacagc tcgcggtgct gatcccggga ctcgactcca ccaaggagga gttcttcaac
    29461 ctcgaggagt tcttcctgaa caggggcatg gccacgcttt ccatggatgg ccccggtcaa
    29521 ggcgaatgcg ggtacgacgt gccgatccgc cccgactacg aggttggcct ctccgcggcg
    29581 ctcgaggccg tggctggtca cgacgacctc gatctcaacc ggatcggagt cgtgggcgtg
    29641 agcatgggcg gctaccacgc gccgcgggcc gccacattcg agccgcgcgt gaaggcggtc
    29701 gtcgggctca gcgggccgtt ctgcttcgga gaaatctggg accagctgcc gcagctcaca
    29761 cgcgagacgt tcaccgagaa gtcgcgcgcg gcctcggagg aggaggcgcg tgacaaggcc
    29821 tgcgagctcg acctggacgg tgtgatcgac gagctcgaca agccagcgct gttcgtcacc
    29881 ggccggctgg atcgcctgat cccgtgggag cagaccgcga agcaggccga gcgcgcgccg
    29941 cagggcgagt tcgtctgctg ggaggagggg aaccacggct gctcgaacat cccttacaag
    30001 gcgcgctcgc tgatggccga ctggatggcg gagcagctcg cgtgacggcg gtcgcgctcg
    30061 ttggcaccgg gatgtgggga ccccgcctgg ccgcggctgc ggagcgggcc gggctgcgcc
    30121 tcgtcacctg cttcagccgc gacgacgagc ggcgcgggga gttcgccgag cgcttctcct
    30181 gccaggcggc ggcgagcttc gaggatgcga tcggccaccc ggaggtcggg ggcgtgatcc
    30241 tggccacgcc gaatgacgtg catgccgagc aggcgctcgc gtgcgcggag cgagggcggc
    30301 acgtgttcgt ggagaagccg attgccgaca caaccgaggc cggggagcga atgcgcgagg
    30361 cgtgcgagac cgcgggcgtc acgctcatgg tcggacacgc gttccggcgg cttggcgcgg
    30421 cccgccgcgc caaggagctg gtcgacgagg gcgcgctcgg gcgcgttgtg ctggcggagg
    30481 cgaacttctc gctgcccggg aagctcggtc cccaggcttg gcggtactac cgggatcgca
    30541 atcccggtgg cccgctcatg cagctcggca tccaccacgt ggacacgctg gctcacctcc
    30601 tgggcccggt gcggcggtcg tctggccgct tcgcgcacgt tcacacgcag gcggagatcg
    30661 acgatgtcgg cgtcgtggtt ttggagttcc agtcgggcgc gctcggctcc gtcaccggca
    30721 gttacgtgtc gccgaagacc ttcgacatcc gcctgttcgg cacggatgcc gtgatcgagt
    30781 accgcaccga catgtccgtc tggccggacg ccgagaagat ggatcccgcg acgacgctca
    30841 cgctctcggg tagccgtgtg gagttcgacg agcgcgacat gctcgccgag gagctggcgg
    30901 agttcggacg atgtatacgc ggggaggcgg agcctgagac gggcgccgac gaggggatag
    30961 cggcgctacg cgtcgttctt gacgcggtgg cttccggtgc ggaggtggcc gcctgatgcg
    31021 cgctgcgatc gtgcgccagg ccggcacgcc cccggaggtg gccgagcgcg acgcgccggc
    31081 gcccggcgag ggtcagacac tggtgcgggt gcgggctgcc acgctcaacc ccgtggacct
    31141 gcacatcgcg ggcgggcgct tcttcaccgg ccctccgcag tttccatacg tgcccggagg
    31201 cgagggcgtg ggcactctgg ccggtgggaa cggcaagcgg gtgcgtttcg agtgctggta
    31261 cgtgggctac ggcggcgacg gctcgctggg cgagctggcg ctcgcggcca ccgagtcgct
    31321 gacagagctg cccgatgaga tcgacgacgc gctcgccgcg gcgctcggca cggtcggcac
    31381 gacggcgtgg cttgcgctcg agcgcgccca gctctcggag ggcgagaccg tgcttgtgct
    31441 gggcgccacc ggggcaatcg gccaggtggc ggtgcaggcg gcgagactgc agagcgcggg
    31501 ccgcgtgatc gcggccggcc gcaacactga acggctggag catgcgcgag agctcggagc
    31561 tgacgcggtc gtcgagctgc gtgacgggat ggggaggcac gagctgacgg cagcgttcag
    31621 ggaagcgggg agtgggggcg tggacgtggt cgtggacccg ctgttcggag aggtggcggc
    31681 cgccgcgctc gaagccggaa acccgggggc gcgcctcgtg aacatcggtc gcgtcgcagg
    31741 caccgagtca gtgttcacgc ccgcgacgct caggaacaag gccgcgatcg gcctctccac
    31801 tcactactcg ccggtcgagg agaagaagcg cgtgtaccgc gtgctggtcg acctcgccct
    31861 gaagggcaag ctcaccctcg agcacgaggt gctgccgctt gatgaaatcg gccaggcatg
    31921 gagcatgcag gccggctcgc cgggtcgcaa gctgatcatc gatcccgccg cctcatgacg
    31981 ctccacgaca ccgagcagca cgtcgtggtc aaccacgcga gcggggagat ggtcggcccg
    32041 atggcaaccg cactgcacct gcgcgacttc ccgaccctgg tgagcgacga gccgccttcg
    32101 cgaggagggg aggatcgcgg gccgacgccg ctggagcttc tgcttgcatc gctctgcgcg
    32161 tgcaccacgg tctccaccgg caggatggcc gccaagctca ggttcgagta ctccgatctc
    32221 acgacgcgtg cggagggcga gctcgacacg cggggccgga agggggccgc cgacgtgccc
    32281 gtgcactacc gcaccgtgag gctgcgggtg aggatcgcga cggaggagtc cgacgcgcgc
    32341 attgcgcggc tcgccgacct cgtcgggcgc tactgcccgg tggacagcct gatgcgcgcc
    32401 gccgttcccg actacgaggt gacgtgggaa cggctgtaga cgccacgcgc gcgcgcctca
    32461 tcgggcagcc gctgccacgg cgcgaggacg agcggattct ccgcggccgg accagctacc
    32521 tggacgacat cgagctgccg gagctggcgc acgcggcctt cgtgcgcagc ccacacgcgc
    32581 acgcgcgcgt gaatgagata cgcgcgccgg cggaagcgcc cgggctgctt accgtcatca
    32641 ccgccgagag cctgggcgag cgcgtgcgcc cgtttcccgt ccaggcaccg cccgagatgg
    32701 agacggccga cgagccgcat cccgtgctgg cggcgggtga gattcgctac gtgggccagg
    32761 cggtggcggc ggtggtggcg gagacacgcg cgctggcgga agatgccgcc gacctcgtcg
    32821 aggtggacta cgagccgctc gagccggtac tcgatccgcg cgcgggcggg ccgcgcctca
    32881 ctcactggac gcgatcgagc ggcgacgtcg agggcagctt cgaacgggcg gaccacgtgg
    32941 tgagcacgag ccactcgatc ccccgggtgg tggcatctcc gatggagacc cgcggcacgg
    33001 ttgcgtcctg ggactcgggc tcggggatgc tcaccatgtg ggtgtccgcg caggacccgc
    33061 accgcccgct ctcgcagctc gcgcactcgc tgcggcggcc ggaggactcc atccgggtga
    33121 tcgtgccgga cgtggggggc gccttcggga gcaagggcgt cgcggccccc gagtcggccg
    33181 cgctggcaat cgcggcgatg gaccttgggc gtccggtgaa atgggctgag gaccggctcg
    33241 agaacttcct gtccgcctac cagggccgcg gcatggacgt ggacgtggag ctggcgctcg
    33301 cggccgatgg ccggatgctg gcgctgcgcg cacggatctt cgccgacctc ggcggctacc
    33361 tcctgacgac cacggcgatt ccggcgcaca cgacggccat gctgatgacc ggcgtgtacg
    33421 acatcggcgc ggccgaggtg aacgtcgttg gcgctcagac ccacaaggtg cccaccgggc
    33481 cgtaccgcgg cgcggggcgg cccgaggcgg cgtacgcctt ggaactcact gtcgacgccg
    33541 ccgcgcgcga gctggagatg gatccggtcg agttgcgccg ggtcaacctc atctcggagt
    33601 tcccgcatga aacgccgctc ggcttcacct acgactcggg tgagtacgcg cgctgcctac
    33661 agcgggcggc cgagcttgct gagttcaccg agcaggacgc cggcaccgga cgccggctcg
    33721 ggactggcgt gggcatgtac gtggagcgtg ccggcgggat gtgggagagc gcgcacgtga
    33781 ccgtggagcc ggacggccgc gtgatcgtgc gcagcggctc gaacccgcac gggcagggcc
    33841 atgacacagt gttcgcgcag atcgccgcgg agcggctggg cgtggacatc ggcgacgtgg
    33901 cgctgcgctt cggcgattcg gcgatcgtcc cccgcggtgt tggcagcttc gccagccgct
    33961 cggtggccat ggggggctcg gcgatcgtgc gtgccatcga cgacatccgc gacaagtgcg
    34021 cgaccatcgc ggcgcacctg ctggaggctc cgccggagga gatcgcgtgg acctccgacg
    34081 gcgggctgtc cgcaggtgat gggcgcgagc tcagcctccg cgagatcgca gccgcggcgt
    34141 accagccgcc gcgactgccg gcgggcatgg agccgggcct ggaggcgtct gcccgcttcg
    34201 cctcgaacca ggtgttctcc tcgggcgcgt acgccgcggc ggtcgcgatc gacgaggcca
    34261 cgggacgcct cgaggttctg aagctcgtgg ccgtggacga cgctggcgtg atcgtcaatc
    34321 cgctgctggc ggacggccag gtgattggcg gtgccgcaca gggtctcggg gccacgctcg
    34381 tggaggaggc ggtgtacgac gagaacgggc agctgcgcac cgggtcgtac gccgactaca
    34441 gcctcatgac cgcggtggag atgccgcaca tcgagacgga cttcgtcgag agcccatcgc
    34501 cgctcaatcc cctcggcgct aaggggatag gcgagggagg cgcgatcggg acgcccgccg
    34561 cgatcggcaa cgccgtggtt caggcgctcg gcgggcgcat cgtgcacccg ccgtacaccg
    34621 ccgagaagct ctggcacgcg atgcagggcg agtcgacatg aagctgaaca acgaattcgt
    34681 cgtggccgcg ccgctcgagc gcacgtggtc cgcgctgctc gacgtgccgc gcgtcgcacg
    34741 cgctttgccc ggcgcgacca tcgactcgga gccggcggac ggcgcctacc gcgggacgat
    34801 gaaggtcaag ttcggggcgg tcacagcgga gtacgccggc accgcgatgc tccaggacgt
    34861 ggacgaggac gagcgggtcg cgagcttccg cgtggaggcg cgcgagcggc gcgggcaggg
    34921 aacggcggcc gccacgatca ccagccgtct cgcggaggac agcgccggca cgcgcgtgtc
    34981 tgttgagacg gacctcgcgg tcacaggtcg ccaggcgcag ctcgggcggg ggatcatgga
    35041 ggatgtggcc ggaggcgtgc tcgagcgctt cgcggacggc ttggagcagg agctcgcggg
    35101 tggcagcccc ggacctgtgc agccgcccgg atccgaagag gcgctcgatc ttggcggggc
    35161 cgtgctcgga ccgctacgcg agcgcgcggc ggtcttcggc gcgggtgtcg cgctcggctt
    35221 cctggcgggc tggctcctgc ggagacgatg aagccggctc ccttcgagta cgcggcgccg
    35281 cgctcggttg acgaggcgct tggcctgctc ggcgaggact cgaaagcgct cgccggcggg
    35341 cagagcctcg tgccgctgct caacttccgg ttcgcgcgcc ctgagctgct cgtggacctg
    35401 aaccgtgttt cggagctctc ctacatcagg cgcagcggcg gcgtgctcca catcggagcg
    35461 ctcacccggc aggccacgct cgagcgctcc ggcgtggtgt ggcagcactg gcccctactc
    35521 gctcaggcgg tgcggcatgc cgggcacccg cagacgcgca gccgcggcac cgtgggcgga
    35581 tccgccgtcc acgccgaccc cacggccgag ctgccagtcg cgctcaccgc gctcgacgca
    35641 cgctttcacc tgagctctgc gcggggccgg cggacgctca ctgcgccaga gttcttcgtg
    35701 gccaccctca tctccgcggt ggagccagac gagctgctcg tggaggtgga ggtgccggcg
    35761 ctgcctgaag gcgcgcgcac tgcgttcgtg gaataccagc ggacgcacgg ggacttcgct
    35821 gtggcgggcg cggcggtggt ccgcggtccg ggcgacggct cggcgatcgc gctgctgggc
    35881 gcgggcacga caccggtccg ggctcacgag gctgagcgcg cgctgacggc cggcgctggc
    35941 gcccgcgagg cggccgagct cgcggctgga gcgctcacgg atggctggag gagagcgctc
    36001 accacggagc tcgtgcgtga ggcgatcgaa agagcggccg catgaggatc ggcgtggaga
    36061 tcaacggaac gctgtatcag ggagaggtcg agccgcgcac gctgctatct gacttcatcc
    36121 gtcaccaggc tggtctccat ggcacgcggg tcggctgcga gcacggggtg tgcggggctt
    36181 gcacggtgca gctcgacggc gagccgatcc ggtcctgtct gatgctcgcc gtgcaggcga
    36241 acggccgtgc cgttcgcacg gtggaggggc tcgccccgga cggcgagctt cagccgctcc
    36301 agcgagcctt ccacgagaag cacgcgctcc agtgcggctt ctgcacgtcc ggcttcctca
    36361 tgtcgctcga gccgttcctc cgcgaccacc ccgaggcggg cgaggacgag atcagagagg
    36421 cgctggccgg aaacctgtgc cgctgcacgg gctacgaggc gatcgtcgaa gcggtgaaga
    36481 gcgcagccga ggagatgcga tgaagcagga gacggacctc gaggcgatgt accgcctctt
    36541 ctcgtggagg atcacgtcca actacgtgcc ccactgggac ttcgcccaga tgaaggccga
    36601 gatatcggag tgggacgagt ggtgccgggt gtggagccgc tacgcgagcc ggcatgccgg
    36661 gctgggcgat gcagccgccg ccgacggcga cgagcgcgcc gccggtgccc actacgtgcg
    36721 ggccggtctc ttctaccact gggcgagctt cgtgttcccg caccgtcccg acgacttccg
    36781 agccgcgctt gccgggatgg gggagtgctg gcgcagggca gctccgttca cggagcctcc
    36841 gatggagctg gtcgatgtgc cgttcgacgg cacgctcctc ccgggatacc tgcgcaagcc
    36901 ggccggagtc gagcgcccgc cagtggccgt gctggtgcct ggggccgact ccaccaagga
    36961 ggagttgttc aacctcagcg agcacatcct ggagcgtggc gtcgccacct tcgttttcga
    37021 cggccccgga cacggggcgg tgagcttcga gctgaagctg cgtcccgact atgaggtccc
    37081 gatcggggca gtggtcgact ggctggagca gcgcgacgac ctcgacaccg cgcggctcgg
    37141 cgtctgcggc atctcgtacg gggggctgtt tgcctgtcgc gccgccgcgc tcgacgcgcg
    37201 cgtgaaggcc gccgtgtcca tgagctcgtg gtacacgccg gccgggcgct tccagaatca
    37261 ggaccacatc tcgcaggtcg gcctgcgcca gtacatgggt gaggatccgg gcgctgtcca
    37321 ggacgccatg acgctggagg gcgtcgcgga caagatcacg gttccgcttc tacaggtcta
    37381 cggaggccgc gatcccgcct cgccgcccga gcacgcgtac cgcgtggaag cggaggcgca
    37441 gggccctacg acgacggtgg tctatgagga tggcgtgcac gtctgcaaca acctctggta
    37501 cgaggcgcgg ccgctggtgg ggcactggct ggcgcgcgcg cttcagcccg cgcgcagcag
    37561 cccgcgctca tagtcgtgca ggctctccgc gccggactcg gtcacgagca gcagctcgcc
    37621 ggtctgcact ccggccgtct cgtcgggcgt gaccacgttc ggttgcacga ccacggtcat
    37681 gcccgcttcg aagctgaagt ccggcacctc cgtcagggcg cggctggtcg agcccagcac
    37741 cggtggtagg tagccgccca cgaagccgtg cacgaggtcg tcgcggatgg tgaagcccgc
    37801 gtcctcgatc acctgcgaag cctgcaccag ctcggcagcg gtggcgcccg cgcgcagccg
    37861 cccggcgatg gcgtcgaagg cggcgtccgc cacttcgtgc agctcctcgt agaggcccgt
//
LOCUS       DASVXS010000009         4887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1276422, whole genome shotgun sequence.
ACCESSION   DASVXS010000009 DASVXS010000000
VERSION     DASVXS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276422"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF752_00545"
     CDS             complement(<1..391)
                     /locus_tag="VF752_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854061.1"
                     /translation="MPSPEREILDFGFDAFNRGDFEAAVRYFHPGIVWRDPAEVPDAS
                     EHHGPDQVRALWDSFSEQWTGMRMDPVEFQEDGERTLVRVHFSGAGRESGAPMELEFF
                     QVWTIRDLQARQVDSFMSRDLAERALHG"
     gene            complement(442..1551)
                     /locus_tag="VF752_00550"
     CDS             complement(442..1551)
                     /locus_tag="VF752_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX8854062.1"
                     /translation="MFDLQAALRRVIEQEGSDLHLKVPSRPLIRTHGILEPIPDSEPL
                     SPEDTEKVFHEMLTNDERLEEFAREHEVDFSYAVPGLARFRVNAFRQRGVISLVCRAI
                     PHTIRTVEELNLPAVIGDLAEEERGIILVTGTTGSGKSTTLAAMIDRINTTKSKHIVT
                     IEDPIEFLHRDKRSIINQREVGNDTTSFKRALRRVLRQDPDVILVGEMRDEETVATAL
                     SAAETGHLVFSTLHTVDAPETVNRIIDFFPPHMHQQVRAMLAGTLKGVISQRLVRTPD
                     GKGRVATCEVLRMTGRVRDMIVNPEETGRLTEAIAEGEYYGMQTFDQALLAHVQAGRV
                     SMEEALTAATSPHDFKLMIASDGQRSTSVDQLLAT"
     gene            complement(1612..2919)
                     /locus_tag="VF752_00555"
     CDS             complement(1612..2919)
                     /locus_tag="VF752_00555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX8854063.1"
                     /translation="MTRTCRTNSSRVRLAAALALACATALLAVGPAQAVPPSASGRLL
                     VGFEKGVSKDRQQSIISSLEGRIAHRFGPIRGGRLVLVRARSSKATDVLRKRLLRNPA
                     VAYAEPDFFQFASATTPDDPYYSLDYAVVNSPQGHDIDAPDAWGTRTSCSKVATLDTG
                     VDTDHPDLTANILKTSDKPNNDKDDDHNGYVDDTYGWNVINGKGSGEDDNGHGTHVAG
                     IIGALGNNANGIAGVCWSTKIVPVKFMNSKGKGSTSNAIDGIHYAVHAGVKIINCSFG
                     SSAKSSSLEDAVQYAKSNGVLIVVAAGNDSENIDKSPIYPASYTDGNILTVAASTSSD
                     TLASFSNYGPKGVDVAAPGDSIFSTYLGGGYKTLSGTSMAAPYVSGVAALLRKQESDA
                     TYSDLRTAIRQHVDKPPALNGKDVYDGRLNASKALGAIASLVH"
     gene            3031..>4887
                     /locus_tag="VF752_00560"
     CDS             3031..>4887
                     /locus_tag="VF752_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8854064.1"
                     /translation="MATDTHAVHHLLEREHELETLSAAAVRAAGGAGGVVLIEGAAGV
                     GKSRLVASARACGRELGMQMLEGRGAVLEREFAFGVVRQLFEGVVTPGAAERRERVFA
                     GAAGLAAALLGDGAAGLTPAPGDASFGALHGLYWLTVNLADEGPVLLAVDDVQWSDRS
                     SLSFLNYLSRRLEGLPVLLVAAGRSPDPEGDELWRQLAEDPVAEVIRPHPLSEAATAE
                     VVRNRLGPDAHDEFCRACHRATRGNPLFLRELVGALADAHVVPTENAAAAVTDIGPSA
                     VSRFVLHRLERIGGSATALARAVAVLGGDVDVGLAAQVAGIEPAEARAVADLLVQADV
                     LAPDQKLGFVHPVVQAAVYEDLMPGERTARHLAVAQLLDEHGAPAERVATHVLQCEPA
                     GEQRWVSVLCSAAEDAAEHGDPQAAVAYLRRALEEPPGEGTRAEVLCELGRWEHANRD
                     VENAQEHLQASLEARGDSEVHARAAAWLSRSAIAFGGPSEARLALDATLAQLETAEGE
                     LALELDAEALALAQLELTLRPLAPERLAEFGRRAAGDSRFEPVARIHAAVERMVHGEA
                     AAAVADEIEACLAGGAPADAYALAMAIDALVVTDRYETAARWLDLALEAARAL"
BASE COUNT          690 a         1621 c         1770 g          806 t
ORIGIN      
        1 ggccgtgcag cgcccgctcc gcgaggtcgc ggctcatgaa cgagtcgacc tggcgggcct
       61 gcaggtcccg gatcgtccag acctggaaga actccagctc catcggcgcc ccgctctcgc
      121 ggccggcgcc gctgaagtgc acgcgcacga gcgtccgctc gccgtcttcc tgaaactcca
      181 cggggtccat gcgcatgccc gtccactgct cggagaacga gtcccacagc gcgcgcacct
      241 ggtcggggcc gtggtgctcg ctcgcgtccg ggacctcggc gggatcacgc cacacgatcc
      301 ctgggtggaa gtagcggaca gccgcctcga agtcgccgcg attgaaggcg tcgaagccga
      361 agtcgaggat ttcgcgttcg ggcgagggca cgcggcccga ttgtcccccg agggtcgcgc
      421 cgtgtcgacc ccgacgccga attaggtggc gagcagctgg tcgaccgacg tggagcgctg
      481 gccgtcggag gcgatcatga gcttgaagtc gtgcgggctg gtggcggcgg tgagcgcctc
      541 ctccatcgac acgcggccgg cctgcacgtg cgcgagcagg gcctggtcga acgtctgcat
      601 cccgtagtac tcgccctcgg cgatcgcctc ggtgaggcgg cccgtctcct ccgggttcac
      661 gatcatgtcg cgaacgcggc cggtcatccg gagcacctcg cacgtggcca cgcggccctt
      721 gccgtcgggc gtgcgcacca agcgctgcga gatgacgccc ttcagcgtgc ccgcgagcat
      781 cgcgcgcacc tgctggtgca tgtgcggcgg gaagaagtcg atgatccggt tcacggtctc
      841 gggcgcgtcc acggtgtgga gcgtcgagaa cacgaggtgg ccggtctcgg cggcggacag
      901 cgcggtggcg accgtctcct cgtcgcgcat ctcgccgacg aggatcacgt ccgggtcctg
      961 acgcaggacg cggcggagcg cgcgcttgaa cgaagtcgtg tcgttaccca cctcgcgctg
     1021 gttgatgatc gagcgcttgt cgcggtgcag gaactcgatc ggatcctcga tcgtgacgat
     1081 gtgcttcgac ttcgtcgtgt tgatccggtc gatcatcgcc gcgagcgtgg tcgacttacc
     1141 ggagccggtc gtgccggtca cgaggatgat cccgcgctcc tcctcggcga ggtcgccgat
     1201 cacggccggg aggttgagct cctcgaccgt gcggatcgtg tgaggaatcg cgcggcacac
     1261 gagcgagatc acgccgcgct ggcggaaggc gttcacgcgg aagcgcgcca gtcccggcac
     1321 ggcgtaggag aagtccacct cgtgctcgcg cgcgaactct tcgaggcgct cgtcgttcgt
     1381 gagcatctcg tggaacacct tctcggtgtc ctccgggctc agcggctcgg agtccgggat
     1441 gggctcgagg attccgtgtg tgcggatcag cgggcgggag ggaaccttca ggtgcaggtc
     1501 cgacccctcc tgctcgatga cgcgccgaag cgctgcctgc agatcgaaca tgctctccct
     1561 gtatcggacg gcgggcggac ggacttgaga gcgtgcgccc gcccaattca gctagtgcac
     1621 aagtgatgcg atggcgccga gcgccttgct cgcgttcagg cgcccgtcgt agacgtcctt
     1681 gccgttgagc gccggcggct tgtcgacatg ctggcgaatg gccgtgcgca ggtcgctgta
     1741 cgtggcgtcc gactcctgct tgcgcagcag cgcggcgact cccgacacgt agggagcggc
     1801 catcgaggtg ccggacagcg tcttgtaacc gccgcccaga taggtcgaga agatgctgtc
     1861 gccgggggcg gcaacgtcga ctcccttggg cccgtagttc gagaacgagg caagtgtgtc
     1921 gctcgacgtc gacgccgcca cggtgaggat gttgccgtcg gtgtaggagg cgggatagat
     1981 cggcgacttg tcgatgttct cgctgtcgtt gcctgcggcc accacgatca ggacgccgtt
     2041 gctcttggcg tactgcaccg cgtcttccag cgagctcgac ttggcgctcg agccgaacga
     2101 gcagttgatg atctttacgc cggcgtggac ggcgtagtgg atgccgtcga tggcgttgga
     2161 cgtcgagccc ttgcccttcg agttcatgaa cttgacgggc acgatcttgg tggaccagca
     2221 gacgcccgca atcccgttcg cgttgttccc aagcgcgccg atgattccgg ccacgtgcgt
     2281 gccgtgcccg ttgtcgtcct cgcccgaccc cttgccgttg atcacattcc agccgtaggt
     2341 gtcgtcgacg tagccgttgt ggtcgtcgtc cttgtcgttg ttcggcttgt cggaggtctt
     2401 caggatgttg gcggtgagat ccgggtgatc ggtgtcgacg cccgtgtcga gggtggcgac
     2461 cttcgaacag ctggtgcgcg tcccccacgc gtccggcgcg tcgatgtcgt ggccctgcgg
     2521 gctgttcacg acggcgtagt cgagcgagta gtaaggatcg tcaggcgtcg tcgcggacgc
     2581 gaactggaag aagtccggct cggcatacgc cacggccgga ttgcggagca gccgcttgcg
     2641 caggacgtcg gtcgccttgc tggaccgtgc ccgcacgagg accagccgtc cgccgcggat
     2701 cggcccgaag cggtgcgcga tccggccctc gagcgacgag atgatgctct gctggcgatc
     2761 cttcgagacg cccttctcga agccgacgag cagccgccca gaggcggacg gcggcacagc
     2821 ctgcgccggg cccacggcga gcagcgcggt ggcgcacgcc agcgcgagcg cagccgcgag
     2881 gcgcacgcgc gacgagttcg tgcggcaggt gcgagtcatg agcccagtgt catgtggcgc
     2941 cgtcacaagg acaacgggga acaccccagg atttgccctg gtttttgctc tccattaccc
     3001 tgagccggga ggcgccgggc tcgcccccag atggccaccg acactcacgc ggtccaccac
     3061 ctgctcgagc gtgagcacga gttggagact ctgagtgccg cggcagtgcg cgcggccggc
     3121 ggagctggtg gcgtggtgct gatcgaaggg gccgccgggg tgggaaagag caggctcgtc
     3181 gcgagcgcgc gcgcttgcgg ccgcgagctc ggtatgcaga tgctcgaagg gcgcggcgcg
     3241 gtgctcgagc gcgaattcgc cttcggcgtc gtgcgccagc tcttcgaagg ggtggtcacg
     3301 ccgggcgcgg ccgagcggcg cgagcgcgtt ttcgcgggcg cggccggact ggccgcggcg
     3361 ctcctcggcg acggcgccgc cggtctgaca cccgcgcctg gggatgcgtc gttcggcgcc
     3421 ctgcacggcc tgtattggct cacggtcaac ctcgccgacg agggcccggt gctcctcgcg
     3481 gtcgacgacg tgcaatggtc cgatcgctcg tcgctgagct tcctcaacta tctctcgcgc
     3541 cgcttggaag ggctgcccgt tctgctggtg gcggcggggc gctcacccga tcccgagggc
     3601 gacgagctct ggcgccagct ggccgaggat ccggtcgctg aggtcattcg tccgcacccg
     3661 ctgtccgagg ctgcgaccgc tgaggtcgta cgcaatcggt tgggcccgga cgcgcacgat
     3721 gagttctgcc gcgcgtgcca ccgtgccacg cgcggcaatc cgctgttcct gcgggagctg
     3781 gttggcgcgc ttgccgacgc gcatgttgta cccaccgaga acgccgcggc ggcggtcacg
     3841 gatatcggcc cgtcggcggt gagcagattc gtcctccatc gactggagcg gatcggcggc
     3901 tccgccaccg cgctcgcgcg ggctgtggcg gtgctcggag gcgacgtcga cgtcgggctg
     3961 gccgctcagg tggccggaat cgagccggcc gaggcgcgag ccgtggccga cctcctcgtc
     4021 caggccgacg tgctcgctcc cgaccagaag ctcggcttcg tccacccggt cgtacaggca
     4081 gcggtctatg aagatctgat gccgggcgag cggacggccc ggcacctcgc cgttgcgcag
     4141 ctgctcgacg agcacggtgc gccggcggag cgggtggcga cccacgtact ccagtgcgag
     4201 ccggccggag agcagcgatg ggtgagcgtg ctgtgttcgg cggcggagga cgcagccgag
     4261 cacggcgacc cgcaggcggc tgtcgcctac ctgcggaggg cgctcgaaga gccaccgggt
     4321 gaaggaacca gggcggaagt tctctgcgag ctcggtcgct gggagcacgc caaccgcgac
     4381 gtcgagaacg cgcaggagca cttgcaggcg agcctcgagg ctcgcggcga ttcggaggtg
     4441 cacgcgcgcg ccgccgcttg gttgagccgc tcggccatcg cgtttggggg gccgagcgag
     4501 gcgcgcctag ctctcgacgc cacgctcgcg cagctcgaga ccgcagaggg agagcttgcg
     4561 ctcgagctgg acgccgaagc cctcgcgctc gcccaactcg agctgaccct gcgcccgctc
     4621 gccccggagc gactcgcgga gttcgggagg cgtgccgccg gcgacagtcg cttcgagccc
     4681 gtcgcgcgta tccacgcggc ggtcgaacgg atggtccacg gcgaggcagc cgcggcggta
     4741 gcggacgaga tcgaggcctg ccttgcgggt ggagcacctg ccgatgccta cgccctcgcc
     4801 atggccatcg acgcgcttgt cgtgaccgat cgctacgaga cagcggctcg ctggctcgat
     4861 ctcgcgctcg aagcggcgcg cgcgctc
//
LOCUS       DASVXS010000010         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1276842, whole genome shotgun sequence.
ACCESSION   DASVXS010000010 DASVXS010000000
VERSION     DASVXS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276842"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF752_00565"
     CDS             complement(<1..253)
                     /locus_tag="VF752_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854065.1"
                     /translation="MGRPLSESAERAAAAVDPAAIAADLGALVRVPSLTGRERPAVEL
                     LATLAEERGLAAVVHQHDLAQLRAADGYPGEEAARDELLG"
     gene            300..373
                     /locus_tag="VF752_00570"
     tRNA            300..373
                     /locus_tag="VF752_00570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:334..336,aa:Thr,seq:cgt)
     gene            1138..1416
                     /locus_tag="VF752_00575"
     CDS             1138..1416
                     /locus_tag="VF752_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854066.1"
                     /translation="MSSKERDRAMRRLEAALVEQDRLGERFDAAVGTSTEFGAYVRLR
                     AAGDQVRALEAWLHWVEDGGYRGLNAGPFELLAESPRTLACIRRGPEG"
     gene            3617..3889
                     /locus_tag="VF752_00580"
     CDS             3617..3889
                     /locus_tag="VF752_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854067.1"
                     /translation="MTSRARDDAGRAGELAEAPRPDNVLALALPVWVETSVARPAGEG
                     NVSGYAIALGWNTPQKSSAYLISDDAIARPVWVAESELVSNSVRRR"
     gene            3889..4812
                     /locus_tag="VF752_00585"
     CDS             3889..4812
                     /locus_tag="VF752_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX8854068.1"
                     /translation="MSRRWRAPTTLAVSLAATALLAVLLVGRRHEFAAAASSAAPWVL
                     GVAVLGQVVALLARSEAWHLSIRAAGGTVERRALYRASSMQVLGSVLSGQLGVAARIA
                     ALRRSSGTRCPQVGTLVAAEFPILAVEATLAGLASFTLIGPLGLPWWAPLIGLVAVTG
                     ASTGLRRLALRTGHAVWKGLAVLRCRRGGSRVIAFVLVAVLAQVARNWLLLHAVGADA
                     SLFDAIAVLIAVVTLGQLPVGPGLGAAAAVLILGRNGVAAAAAAGVLMTATGTVGGMC
                     FAAWAGADRLLSAARAVRAAVRIPAVAGPEA"
BASE COUNT          759 a         1645 c         1787 g          806 t
ORIGIN      
        1 cgccgagcaa ctcgtcccgc gcagcctcct cgccggggta gccgtccgcc gcgcgcagct
       61 gagcgaggtc gtgctgatgg accactgccg cgagcccacg ctcttccgcc agcgtggcca
      121 gcaactcgac cgccggtcgc tcccgccccg tcagactcgg cacgcgtacc agcgcgccga
      181 gatccgccgc gatcgcggcg gggtcgaccg cggccgcggc gcgctccgcg gactctgaga
      241 gaggtctgcc catgggtccg gtccgcgagc gtactgacgg ctagactcac gcagtcgctg
      301 ccgacgtagc tcagttggcc agagcaccgc tctcgtaaag cgggggtcga gggttcgaat
      361 ccctccgtcg gcttgttgag gaaaggcctg caaatcaggc atttcttatg gcggctgacg
      421 cacgctcact ttcaagcggg ggtcgtactc gacgttccgc tctggaaagc gagatccgtt
      481 cgcttcgtag gtcgtgagtg gagccgagct ggagcggggg tcgaagtcca gggcggcctc
      541 gttgccgttt gatgctcacg agtgattggt ccaacttgtc ggggtgttca cgacttgcgc
      601 tgcacggaga cacctcctct ccgcgcgacg cgggctaggg atgcccgttg tcccgaatcg
      661 ccaaaagcag gagcctgacc acgcggccgc tcgtgaggat ctgcagtagt cccgcaggtg
      721 gctcgtccga attcctgctc tctggcggag caggagggcc acgaggctgc tgaagacttc
      781 cactgtcttt ccgaacgagc actccggtgt ggctcgtcga gagccctgga cagtcctgct
      841 tcgcggcgga ggcggacgtc gcgggagtct ctccgacagc gctcgctgct cgccggagtg
      901 ggagccagtt cggtgccggc cgagacgctc gcatatcgcg tggggagcgg cggactcggt
      961 gcggagggct agcgagacga ctggcggaac tcgcgctatt ccggtgagat gggtctcgcg
     1021 cttcaaactg ccactcatcg gagtagtctc tgttctaagg actgaatcgt tcgttaggtc
     1081 tcactctctc tcttcctttc gtgcggcccc ccacagaggg actaaaggtt gagggccatg
     1141 tcaagcaagg aacgcgaccg cgcaatgcgg cggctcgagg cggcgctggt cgagcaggac
     1201 cggctgggcg agcgctttga cgccgccgtc ggcacgtcga ccgagttcgg agcctacgtc
     1261 cggctgcggg ccgcgggcga tcaagttaga gcgttggagg cctggctcca ctgggtcgag
     1321 gacgggggtt accgagggct caacgcgggc ccgttcgaac tgctcgccga gagtccgcgc
     1381 accctcgcct gtattagaag aggaccggaa ggttgagatc atgacgcgca cagaacgaca
     1441 ccgcacggcg acagggccgg cacaacggag gggcgagctc catcgcgccg atggcgaccg
     1501 ccgggttggg caggccatca aggccaggcg cgaactggcc acccagcgag ttggtggccg
     1561 tgcctggata aacggccgcg aggtcggcgg tgcagatcct cgctacatgc accttgaggc
     1621 ttcgcacgac taagcaagcc gcccaagcgg cgagaacggg aacacgccgg gccgccaact
     1681 gtctggcgtg cgtcccgtga agcggggtgt ccgcagccta atgaagaagc tgctgcccac
     1741 ccgtggggcc tgacgaggtc gccgtatgct gccgccgtgc gggcgcgctc ctagaggacg
     1801 ggcagcggag tatcgttctg gtcaagctgg cctcgcagcc tcgatgccag ccgaggcgcg
     1861 atggccgccg aaagcgcctt aggcgcggac gaggcgagga gccgaggcga atggcgttcg
     1921 ctgtggggcc agcagctatg agcgacgacg ctggccgccg gattatgcga gtccgggcac
     1981 gtaggtcagg gcgcgagccg gcacggcgga tagcgatcgg tgacgagcag gaaggcgtag
     2041 gcgccgacgc ggttcgcgca gcgcatcacc cccaccaaga aatcgagatt ccccgcggta
     2101 gcggcccgtg aagaggatcg cgaaccacgc gcctatcgcg gcgaagaaga ccctacgtcg
     2161 cctcggcgcc gcctgccgac ccgtcttgtc ctggtgcgct gtacgggcgc tcagatggat
     2221 cgacatctgg gttggtcgcc cattgcggcg gcctgccgca ggccatccca ggcaggagca
     2281 tgagcgtgtg tcgctgacat ccgaaaaccg gacgcggcat tgatgggcgt atgccgcgag
     2341 cgataagccg ggcgagcgag cggcgctggc ctatgagcgg gaagtgcgtg cgtaccggcg
     2401 agctggcgcc gcacacgccg cgcttctcgg ttggagctcg ccgcggtcga gtcttgggag
     2461 cggcgtggta agccgcaagt cgccgagcga caccggcacg ccgcacaaga ccacagcaga
     2521 ttggcggcag aagaccgaaa acgggccgac gcgaagcctc tcgacggcta cggggcgtct
     2581 gttctttggc taagcgaggc gctcaaaggc ggtgtcaatc tcggccgcgt tggcgctgaa
     2641 tcacagcgtc agcgcgcctg accgcctcgg cggcgtccga tgccagttcg tgcagcttga
     2701 cagccgcctc atggatgtcg gcggcgcgtt cctcttccca ctcacggctt cgctcggccc
     2761 gctcgcgtgc atcgtcggca cgcgtcttcg cggcatcccg gcgcgcgcgc ccctcgctgc
     2821 accttctctg acacgaccct gtccgtaccg gccatgcgct tgggcaagct gaggtgtacg
     2881 cgaccgaggc cttcgatggc agagccgagt cccccaacct cctcccgttc cgcgactgag
     2941 gctcgcctcg acgatacgcg atccggtcgg gcacgcccgc accgtgacga agacagtcct
     3001 ggtcggcctg ctgaacaagc actgagtctt ggcaaagcag ttggccgcga cgccccgcat
     3061 tcgccgccgg cacggaatcg cttgcagcta ggcgcaaccc ggctccgcgc ggaaggcagc
     3121 ggcgggccac ttggtggcgg cccttcgtgt tctgagcagc gcgtgcagtt gcttgcgagc
     3181 tgccgattgc tccccaatgc cgccgggcgg tcgcgccgtg gatagcacag aggccccaca
     3241 cccagccgaa ctcctcgctc tgcctccggt gttccgctat agaaacaacc agctcacacc
     3301 ggaagcaacc aagaacgaaa gaacgtcgac aagcggtcgc ctgctggtag cagggcccgc
     3361 acgtgagcct accgtagtgg agagtctcaa cgcgctggag tgcattgccc acagtctgta
     3421 cgatcgcgag ggcgggctac acttggttaa gtcgttcgcc tctgggttct cctgtctcct
     3481 ttgatcctcc ggcgtgctcg ttcgtagagg aggccgcaac agaggaggca gtcgtcgtcc
     3541 gtttcgcctt cgccgggccc tcgcgcacca ggatgtcggg gccgcagcgg cgactgccgc
     3601 cttggggaac aggaccgtga cgagccgggc gcgggatgac gccgggcgcg cgggcgaact
     3661 tgcggaggcg ccgcggccgg acaatgtgct cgcgctggcg ctgcctgtct gggtggagac
     3721 ttccgtggct cgccccgcgg gcgagggcaa tgtgagcggc tacgcgatcg cgctgggctg
     3781 gaacacgccc cagaagtcga gcgcgtatct gatcagcgac gacgcgatcg cccgtccggt
     3841 gtgggtggcc gagagcgagc tcgtgagcaa ctccgtccgg cggcgatgat gagtcgtcgc
     3901 tggcgcgcgc ccacgacgct ggcggtgtcg ctggccgcga cggcgctgct ggccgtgttg
     3961 ctcgtcggcc ggcgtcacga gttcgctgcc gcggcctcta gcgccgcgcc gtgggtgctt
     4021 ggcgtcgcgg tgctcgggca ggtcgtcgcg ttgctcgcgc ggagcgaggc gtggcacctg
     4081 tcgatcaggg cagcgggcgg aacggtcgag cgccgggcgt tgtaccgcgc gtcgagcatg
     4141 caggtcttgg gcagcgtgct gagcggacag ctgggggttg cggcgcgcat cgccgcgctc
     4201 cggcgctcgt cggggacgcg ctgtccgcag gtcgggacgc tggtcgccgc cgagttcccg
     4261 atcctcgcgg tcgaagcgac gctcgcgggc ctggcgtcgt tcacgctgat cgggccgctc
     4321 ggtctgccgt ggtgggcgcc tctgatcggc ctcgtcgcgg tcacgggcgc cagcacgggc
     4381 ctgcgtcggc tcgcccttcg caccggtcac gcggtctgga aggggctcgc ggtgctccgc
     4441 tgccggcgcg gcgggagccg cgtgatcgct ttcgtgctgg tagcggtgct cgcgcaggtc
     4501 gcgcgcaact ggttgcttct ccacgccgtc ggcgccgacg cgtccctctt cgacgcgatc
     4561 gcggtactga tcgccgtcgt caccctcggt cagctaccgg ttgggccggg gttaggcgcg
     4621 gccgccgcgg tcctcattct cggcagaaac ggcgtcgcgg ccgcggcggc cgccggggtt
     4681 ctgatgacag cgacgggcac ggtcggcggc atgtgcttcg cggcgtgggc gggggccgac
     4741 cgcctgctga gcgccgcgcg cgccgtccgc gcggcagtac ggataccagc agtcgcgggg
     4801 cccgaggcgt gacggacaag cgcgagccgc cgcagcagcc acctccctcc gacgggaagc
     4861 gcgtgatggg aaatcagcgc tcggaggcca gtcgcaagag cttcctgcgc gaactcaagc
     4921 gagcggcccg cgctcgccta gacaagcgaa gcccccagct gtagccgggc ttcgagggct
     4981 gattactgct agcgtcg
//
LOCUS       DASVXS010000011         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_2743, whole genome shotgun sequence.
ACCESSION   DASVXS010000011 DASVXS010000000
VERSION     DASVXS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2743"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VF752_00590"
     CDS             complement(<1..1242)
                     /locus_tag="VF752_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8854069.1"
                     /translation="MSDAVQTERDLEVLLKTEDRFPPPEDFKQQANFSDPAVYDEAES
                     DWQGWWESWAKKLDWFEPWQTVLEWNPPWAKWFKEGKLNASHNCLDRHVEAGKGDKVA
                     FHWVGEDGATRDVTYADMLEETKRFANVLKSIGVGKGDVVGIYMPMVPETPTAMLACA
                     RIGAVHNVVFGGFSVEAVRERMEVSDAKVLVTADATLRRGKPTPMKAEVDKILGDLPK
                     MEHVVVVRRTEEDVPMTEGRDLWWNECVEHADTECPPEPLEAEHPLYILYTSGSTAKP
                     KGILHTTGGYLTGVAATHYAVFDLKAETDVYWCAADIGWVTGHSYIVYGPLCNGATSV
                     LYEGAPDYPDKDRWWQIVEDYKVTVFYTAPTAIRACIKWGREYPEKHDLSSLRLLGTV
                     GEPINPKAWIWYHKVIGGERCP"
     gene            complement(1311..1991)
                     /gene="rpe"
                     /locus_tag="VF752_00595"
     CDS             complement(1311..1991)
                     /gene="rpe"
                     /locus_tag="VF752_00595"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX8854070.1"
                     /translation="MARALPDGRRVAPSILSADFSRLGDQIREVLDAGARVIHFDVMD
                     GHFVPPITIGPLVLESIAGLVHDAGAWLDVHLMVERPERQVPDFARAGADSINVHFEA
                     TPHVHYALKSVREQGCLAGLAINPATPAEAVTAAVDELDILLCMTVNPGWGGQAFIES
                     STAKVARLRELLPERVCIAVDGGVDTDTVGRLAEAGASLFVAGSAVFGSPDPADSYRR
                     IADAAGAA"
     gene            complement(1994..>2618)
                     /locus_tag="VF752_00600"
     CDS             complement(1994..>2618)
                     /locus_tag="VF752_00600"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX8854071.1"
                     /translation="EAAATSEEEVRSAVSVLPPDERAVMLAELVGERENRRHKPGRGF
                     EALRYRFEIVGDYGAFRDLQRHRMLTVQWQPLTPYLGAGVPEELDQAGVGDDYRAALE
                     RSEAEYERLVDIGRAEVAPYALALGFRIRYVLDMNAREAMHLIELRSGREGHPTYRAV
                     AHELHRQIAQVHPGVAAAMTHVDRDTEPRLERMLSEIRAHARRAERV"
BASE COUNT          388 a          933 c          856 g          441 t
ORIGIN      
        1 cgggcagcgc tcgccgccga tcaccttgtg gtaccagatc cacgcctttg gattgatcgg
       61 ctcgccgacc gtgccgagca ggcgcagcga cgagagatcg tgcttctccg ggtactcacg
      121 gccccacttg atgcaggcgc ggatcgcggt cggcgccgtg tagaacaccg tgaccttgta
      181 gtcctcgacg atctgccacc agcggtcctt gtccggatag tcgggcgcgc cctcatacag
      241 caccgaagtt gctccgttgc acagcggccc gtagacgatg tagctgtggc cggtcaccca
      301 gccgatgtcc gcggcgcacc agtacacatc ggtctccgcc ttcaggtcga acaccgcgta
      361 atgcgtggca gcgacgccag tgaggtaacc gccggtcgtg tgcaggatgc ccttcggctt
      421 cgctgtcgag cccgaggtgt agaggatgta gagcgggtgt tccgcctcga gcggctcggg
      481 cgggcactcc gtgtccgcgt gctcgacgca ctcgttccac cagagatcgc ggccctcggt
      541 catcggcacg tcctcctcgg tgcgccgcac cacgaccacg tgctccatct tcggcaggtc
      601 gccgaggatc ttgtcgacct cggccttcat cggcgtcggc ttcccgcgcc tcagcgttgc
      661 gtcggcggtc accagcacct tcgcgtccga tacctccatg cgctcgcgca ccgcctccac
      721 cgagaagccg ccgaacacca cgttgtgcac ggcgccgatg cgggcgcacg cgagcattgc
      781 ggtgggcgtc tcgggcacca tcggcatgta gatgccgacg acgtcgccct tgccgacgcc
      841 gatcgacttg agcacgttcg cgaagcgctt cgtctcctcg agcatgtcgg cgtaggtgac
      901 gtcccgcgtc gcgccgtcct caccgaccca gtggaacgcg accttgtcgc ccttgccggc
      961 ctccacgtgc cggtcgaggc agttgtgcga ggcgttcagc ttgccttcct tgaaccactt
     1021 cgcccacggc gggttccact cgagcaccgt ttgccacggc tcgaaccaat caagcttctt
     1081 tgcccaactc tcccaccagc cctgccaatc gctctcggcc tcgtcgtaga cggcggggtc
     1141 tgagaagttc gcctgctgct tgaagtcctc gggcggtggg aagcgatcct cggtcttgag
     1201 caggacctcg agatctcgtt cggtttgtac ggcgtccgac attcgcgcct ccctcgtctc
     1261 ttccacggcg aggttacccg ggcccttggg tcaggattcc tgttacggag ttaggcagcg
     1321 cccgcggcgt cagcaatccg acggtacgag tcggccggat ccgggctgcc gaaaaccgcc
     1381 gagcccgcca cgaacagcga ggcgccagcc tcggcgaggc ggcccaccgt gtcggtgtcg
     1441 actccgccgt caaccgcgat gcacacgcgc tcggggagca actcacggag gcgcgcgacc
     1501 ttcgccgtcg agctttcgat gaaggcctgg ccgccccagc ccggattcac cgtcatgcac
     1561 agcaggatgt cgagctcgtc gacggccgcg gtgaccgcct ccgcaggcgt cgccgggttg
     1621 atcgcgagtc cggcgaggca gccctgctcg cgcaccgact tgagcgcgta gtgaacgtgt
     1681 ggggtggctt cgaagtggac attgatcgag tccgcgcccg ctcgtgcgaa gtcgggcacc
     1741 tggcgctcgg gtcgctcgac catcaggtgc acgtcgagcc acgcgcccgc gtcgtgcact
     1801 agcccggcaa tcgactcgag caccagcggc ccgatcgtga tgggcggcac gaaatggccg
     1861 tccatcacgt cgaagtggat cacccgcgcg ccggcgtcga gcacctctcg tatctgatcg
     1921 ccgagtcgcg agaagtcggc cgacaggatg gagggcgcga cacgccggcc gtcaggcagc
     1981 gcgcgcgcca tcgctacacg cgctccgccc gccgcgcgtg cgcccgtatc tcggagagca
     2041 tgcgctcgag gcgcggctcg gtgtcgcggt ccacgtgcgt catcgccgcc gcgacccccg
     2101 ggtgcacctg cgcgatttgc cggtgcagct cgtgggctac cgcgcggtag gtgggatggc
     2161 cttcgcggcc ggagcgaagc tcgatcaggt gcatcgcctc gcgcgcgttc atgtcgagca
     2221 cgtagcggat gcgaaaaccc agggcgagcg cgtagggcgc gacctctgcg cggccgatgt
     2281 cgaccagtcg ctcgtactca gcctccgagc gctcgagcgc cgcgcgatag tcgtcgccta
     2341 cgcccgcctg atcgagctcc tcgggaacgc ccgcaccgag atacggagtc agcggctgcc
     2401 actgcacggt gagcatgcgg tggcgctgga ggtcgcggaa ggcgccgtag tcgccgacga
     2461 tctcaaagcg atagcggagc gcctcgaagc cgcgccccgg cttgtggcgg cggttctccc
     2521 gctcgcccac gagctcggcg agcatcaccg cgcgctcgtc cggcggcagc acgctgaccg
     2581 cagagcgcac ctcctcctcc gaggtggccg ccgcctcg
//
LOCUS       DASVXS010000012         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_923339, whole genome shotgun sequence.
ACCESSION   DASVXS010000012 DASVXS010000000
VERSION     DASVXS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923339"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF752_00605"
     CDS             <1..543
                     /locus_tag="VF752_00605"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8854072.1"
                     /translation="MSLFHANKPVVCKVHGFCVAGGTDMALCSDLLVIEDEARIGYPP
                     ARVWGVPTTALWADRIGMQRAKRLLFTGDMISGRQAVDWGLAIECAPAAELDERFEAL
                     LERIAHVPVNQLMLHKLLLNQAAFAQGLHATQVLGTVFDGIARHTPEGYAFQQRAADA
                     GFKQAVRERDEPFGDSQPKG"
     gene            560..1918
                     /locus_tag="VF752_00610"
     CDS             560..1918
                     /locus_tag="VF752_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854073.1"
                     /translation="MYRWGFVGPERVADAAFDSQLTITLREAPGDATELTLVHERLET
                     IDAAMPGMADATSHGRAYALRSTPSLPHSRKPRDDRLPGVDAPAAAPERHIVLGLVAE
                     PGVSAELAKDLAAELPEHLSQHVSDRVEWEVPVVVEPLDTAGGGGIELIEAARRLMMR
                     EGWDMGIALTDLPIRIGRRPVVADASAQHRIGIISLPALGALHLNRRAREAVLRLVEG
                     LVGESMELDAAGAARTRRVGRRLVEMAAPVRRVAPADGDVDIRFVAAVVRGHLRLLAG
                     MVRANRPWRLIAGLSRALAASLAAVAFAIVTADIWRLADDLSWPRLLAITVMTIVATV
                     VWLIADHDLWEWPSGRASREQVVLFNAATAATVALGVVFLYLALYVLTLVAAELVITS
                     HEFGSRLGHPVDFGDYVKLAWFVSSLATVGGALGSGLESDEAVREAAYGYRPDRGSEQ
                     RL"
     gene            complement(1921..2700)
                     /locus_tag="VF752_00615"
     CDS             complement(1921..2700)
                     /locus_tag="VF752_00615"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX8854074.1"
                     /translation="MPFELIFPWHGVWDSLPAARAAAITFDLLTMVALFFVGRRLRPG
                     REGRLLGVVLAYAWAAFPYAVYTTQSAANDALVSLCVTAALAAFALPWLRGAFIGLGA
                     AAKFAPLALAPLFATGRESLGTRNVRQAIVFVVALAVVLLATFVPLAPPGGVHELYDR
                     TVGYQASGSSPFSIWGLAPGLHPLRPVVEAAAALLALGVAVVPRRRSLVQVAALGAAV
                     LVAIQLPAKHWFYIYAVWFAPLMFAALFGEHTRLFGRMQRR"
     gene            complement(2685..3074)
                     /locus_tag="VF752_00620"
     CDS             complement(2685..3074)
                     /locus_tag="VF752_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854075.1"
                     /translation="MSLYKDGAEKAQVYVSDADARATETWTGRQVDTYLARGYSGQFV
                     GKLNAPWLWLPLCLLFVLPFIDPRRPFRLLHLDLLVLVAGLGLSHFFFERGNIDASVP
                     LVYPVLAYVLARMLYAGLRPSRTCRSS"
BASE COUNT          551 a         1196 c         1242 g          446 t
ORIGIN      
        1 atgagcctgt tccacgccaa taagcccgtg gtgtgcaagg tgcacggctt ctgcgtggcg
       61 ggtggaacgg acatggcgct gtgctcggac ctgctcgtga tcgaggacga ggcgcgcatc
      121 ggctacccgc ccgcgcgcgt gtggggcgtg ccgacgacgg cgctgtgggc cgaccggatc
      181 ggcatgcagc gcgccaagcg gctgctcttc accggcgaca tgatcagtgg ccgccaggcc
      241 gtggactggg gcctcgcgat cgagtgcgcg ccggcggccg agctcgacga acgcttcgag
      301 gccctgctcg agcgcatcgc acacgtgccg gtgaatcagc tcatgctcca caagctgctt
      361 ctgaaccagg cggcgttcgc gcagggtctg cacgcgacgc aggtgcttgg caccgtcttc
      421 gacggcatcg cgcgccatac acccgagggc tacgcgttcc agcagcgcgc ggccgacgct
      481 ggcttcaagc aggcggtgcg cgagcgcgac gagccgttcg gagactctca accaaaaggc
      541 tgacaattcg ctgatcttcg tgtaccgctg gggcttcgtc ggccccgagc gagtggccga
      601 tgcggccttc gactcgcagc tcacgatcac gctgcgggag gcacccggcg acgcgaccga
      661 gctgacgctc gtgcacgagc gtctcgagac gatcgacgcg gcgatgcccg gcatggccga
      721 cgcgacgtca cacggccggg cctacgcgct gcgctcgaca ccctcgctgc cacactcgcg
      781 gaagcctcgt gatgacagac tgccgggcgt ggacgcgccg gcagccgcgc ccgaacgaca
      841 catcgtgctc ggcctcgtgg ccgagccggg cgtgtccgcc gagctggcaa aggatctcgc
      901 ggctgagctt ccggagcacc tgtcccagca tgtgagcgac cgcgtcgagt gggaggtgcc
      961 cgtcgtggtc gaaccactcg atacggccgg cggtggaggc atcgagctga tcgaggccgc
     1021 gcgccggctg atgatgcgtg agggctggga catgggcata gcgctcacgg atcttccgat
     1081 ccgcatcggg cgcaggcccg tggtggcgga cgcgagcgcg cagcaccgca tcgggatcat
     1141 ctccctcccc gccctgggcg cgctacatct gaaccgcaga gcgcgcgaag ccgtgcttcg
     1201 gctggtggag ggcctcgtcg gcgagagcat ggagctcgac gccgccggag ctgcgcgcac
     1261 tcgccgcgtc ggccgccggc tcgtggagat ggccgcgccc gtgcgacgag tggcacccgc
     1321 ggacggagac gtcgacatcc gcttcgtcgc ggcagtggtc cgcgggcacc tgcgcctgct
     1381 cgccggcatg gtccgcgcga accgcccctg gcggctgatc gcagggctgt cgcgggcgct
     1441 cgcggcctca cttgcggcgg tcgcgttcgc gatcgtgacc gcggacatct ggcgcctcgc
     1501 cgatgacttg agctggccgc ggctgctcgc aatcacggtc atgacgatcg tcgcgacagt
     1561 ggtctggctg atcgctgacc acgacctctg ggagtggccg tcgggccggg cgagccgcga
     1621 gcaggtggtt ctgttcaacg cggctaccgc cgccacagtc gcgcttggcg tggtcttcct
     1681 gtacctcgcg ctgtacgtgc tgacactcgt cgcggccgag ctcgtgatca cgagccacga
     1741 gttcggaagc cggctcggcc acccggtgga cttcggcgac tacgtgaagc tggcgtggtt
     1801 cgtgagctcg ctggcgaccg tgggtggcgc tctcggctcc ggcctcgaga gcgacgaggc
     1861 agtgcgcgag gccgcgtacg gataccgtcc ggaccggggc agcgaacagc ggctctagat
     1921 ctagcgccgc tgcatgcgcc cgaataggcg cgtgtgctcg ccgaacagcg ccgcgaacat
     1981 gagcggggcg aaccagaccg cgtagatgta gaaccagtgc ttggccggca gctggatggc
     2041 cacgagcacg gcggctccga gagccgcgac ctgcacgagg ctacgacggc gcggcaccac
     2101 cgcgacaccg agggcgagca acgcggccgc cgcctccacg accgggcgga gcggatgcag
     2161 gcccggcgcg agcccccaga tcgagaacgg cgagctgcca ctcgcctggt agccgaccgt
     2221 gcggtcgtag agctcgtgca ccccgccggg cggggccagc ggtacgaagg tcgcgaggag
     2281 caccacggcg agcgcaacca cgaagacgat cgcctgacgc acgttgcgcg tgccgagtga
     2341 ctcgcgaccc gtcgcgaaca agggcgccag cgcaagcggc gcgaacttcg ccgcggcgcc
     2401 gagcccgatg aacgcgccgc gcagccacgg cagcgcgaac gcagccagcg cggctgtcac
     2461 gcacagcgac acgagcgcat cgttcgccgc cgactgggtg gtgtagacgg cgtacggaaa
     2521 cgcggcccac gcgtacgcga gcacgacacc gagcaggcgg ccctcgcggc ccgggcgcag
     2581 acgccgcccg acgaagaaca gcgcgaccat cgtgagcagg tcgaacgtga tcgccgccgc
     2641 gcgcgccgcc gggagcgagt cccagacgcc gtgccacggg aagatcagct cgaacggcac
     2701 gtacgcgatg ggcggaggcc ggcgtagagc atgcgcgcga gcacgtacgc cagcaccggg
     2761 tagacgagcg gcaccgacgc atcgatgttc ccgcgctcga agaagaagtg cgagaggccg
     2821 agtcccgcca ccagcacgag caggtcgagg tgcagcagac ggaatgggcg acgcgggtcg
     2881 atgaacggaa gcacgaacag caggcagagc ggcagccaca gccacggcgc gttgagcttt
     2941 ccgacgaact ggcccgaata gccgcgggcg agataggtgt cgacctgccg accggtccag
     3001 gtctccgtcg ccctcgcatc ggcgtcgctc acgtacacct gcgccttctc ggcgccgtcc
     3061 ttgtagaggc tcactcgcca ggagccctcg ccgcgagtcc agacccgagc gcgtcccgcc
     3121 gctcacgccc ttcgccgccg cggccgcgcg agcgatccgc acgacctgcg cgcccgtgag
     3181 ctcgaagccg gggggcctcg gcgcgaggga gcgcacgcga cgcagcggtt gcgcgggctc
     3241 ccggatgccg atgatcgcca ggtgcgatcg atcagctggc cgccgcctcc gggcacggcg
     3301 gcgcccgacc ggacctgcgc gtgagccgga ccggcgagtg agagggccgc ggcgaagagc
     3361 accgcggccg ccgcagctat tgctgcgaga cctctaggaa ggcctcatag cggcgcacgt
     3421 cgcgctcgca gcgtc
//
LOCUS       DASVXS010000013        13315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_214196, whole genome shotgun sequence.
ACCESSION   DASVXS010000013 DASVXS010000000
VERSION     DASVXS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13315
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214196"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VF752_00625"
     CDS             <1..642
                     /locus_tag="VF752_00625"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8854076.1"
                     /translation="PHARRIRWGWPWRGPMPRPPRCCRSCATRLDVLLRRHLIAARRT
                     LLGEAADTGYETQRLAVGFVDLVGSTALAQQLSTRELGAVLTEFETLAADIVAAAGGR
                     VVKLIGDEVLYTAADPRSACRIAVEMTSRLADHPRVPPARAGVASGEVLLRDGDVFGP
                     TVNLAARAVKVARPGEVVIPAALAGEAGITARRLGSMTLRGFPAEVELARVAP"
     gene            772..1647
                     /locus_tag="VF752_00630"
     CDS             772..1647
                     /locus_tag="VF752_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854077.1"
                     /translation="MTLNVGIGLALICAALTQLGFLCKHRGANAVPAIALGRPLQSAR
                     ALLRSRWFAIGLGVTAGAWLMHVAALAIAPLSFVQAVLSTGVVLLAVLGASLFGCKVS
                     RRQWLGVAMTAIGLVLLVLTLPHRGGSHSAYAWPALLAFQGGMLVVGALLIAAPRLGA
                     PSHHHGTALGAAAGILFGVSDVAVKALTGLAGHGALAVVASPWLIVAGTAAVLAFLAS
                     ARGFQQGDAVPVIACTSTAANVTCIVGGIVVFGDALASHTLLIIVQVIAFALVAVAAM
                     LTPTAHGRAPSQAAA"
     gene            1706..2881
                     /locus_tag="VF752_00635"
     CDS             1706..2881
                     /locus_tag="VF752_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8854078.1"
                     /translation="MAVPTAPGAEAAAARVVGRLVADIEGELDALVARIVERIRDEIP
                     DFRRLPVEALQRAVRGNVARALAALRDLRPPTPEELERAAEIGRERAEQGLTVDAVLH
                     AYRISVTAVWSRFGELARERGADVASVLAFSETLWLWADAVMDIVGAAHRQVELQLAR
                     EEQQHRDAFVLALLMGTIDAADLRRDSAAFGLDPECDYIPFRARSTHGEGAPPLPSRV
                     ALALAGDRGLVTALDHDLIGIAARPPVPIPEVAAGIGPPARLAALPGAFALASRALQT
                     ALAFGQEGVFALSDLSIRPAILADDALGDAFVSRYLEPLGALGRLGADLEATLRAWFE
                     HGMRIDDTARRLHMHPNTLRHRLNRFEEATGANLRHPTDLIELWWALERRRLGAESR"
     gene            complement(2886..4151)
                     /gene="aceA"
                     /locus_tag="VF752_00640"
     CDS             complement(2886..4151)
                     /gene="aceA"
                     /locus_tag="VF752_00640"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX8854079.1"
                     /translation="MTSRAGNGSHDVPGRWDGIKRPYAPEDVERLRGTVHIEYTLARI
                     GAERLWDMLGRDEPVRTLGALTGQQAVQEVKAGLPAIYVSGWQVAADANLAGEMYPDQ
                     SLYPANSVPEIVRRINHALQRADEIEHAEGNDGIDWFAPIVADGEAGFGGALNVFELT
                     KAMIDAGAAGVHYEDQLASEKKCGHMGGKVLLPTSHAVRNLIAARLAADVLGVPTLIV
                     ARTDALDADMVLSDGDPADHEFITGERTEEGFFRTRSGMDQAVARARAYAPYADLLWC
                     ETSTPDLGEAREFARAVHAEHPGKLLAYNCSPSFNWKAKLGAEGIASFQDELGGLGYK
                     FQFVTLAGFHTLAHSMFDLARRYRESGMTAYADFQDGEFASEREGFTATQHQREVGAG
                     YFDEVAQAITGGDTSTAALAGSTEEQQFR"
     gene            complement(4199..5287)
                     /locus_tag="VF752_00645"
     CDS             complement(4199..5287)
                     /locus_tag="VF752_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEX8854080.1"
                     /translation="MFWASLSRHERRTLAAMVAAVAGLHVLGFVALVAFSAPRPYHLG
                     TAPVLPIGIGVTAYTLGLRHAFDADHISAIDNTTRKLMAEGKRPLSVGFWFSLGHSTV
                     VFALVLLISIGVRSLDGPVQDGHSSLRQTTSTVGTAVSGGFLYLIAAINIVILLGIVK
                     VFREMRSGRFDETGLERQLDSRGLMNRLFGRLSKAVAKPRQMYPVGLLFGLGFDTASE
                     VALLVIAGGAGAAGLPWYAILCLPILFAAGMSLMDSIDGSFMNFAYGWALSKPVRKVF
                     YNLTITGLSVAVALVIGTIEVGGLISSKLGLSGTFWNSFENVDINFLGLVIVVMFVAT
                     WVIALLIWRLGRIEERWSAHLKDSGPVD"
     gene            complement(5333..6325)
                     /locus_tag="VF752_00650"
     CDS             complement(5333..6325)
                     /locus_tag="VF752_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX8854081.1"
                     /translation="MSNHFSAAMLKFPGDDARLDLTDLFVFESAESPGKTVLIIDVNP
                     FMTGADFHPDAVYRINVDNDGDTQADAAFSFVFSESNGGSQTGTVRYATGSQAREAEP
                     VGEVLIEGTPVSFDTMATPVEAGSCRLFIGVRSDPFFADGEGAFHDFQFTGDDTFAGK
                     NILSMALEVPNDMLGPGPEVGLWGTVSVRRGGKLVQVDRIGNPSFNPFFVDELKNEFN
                     AGHPANDVADYLEPWSRLLESRGYPPAEARDTVLTVLPDILHYDRNRPAHYPNGRALT
                     DDVYDTRMAFLTYGRVTSDGVGPHDDYLAEFPFLGPPNAEAPDQDTGGGALLTS"
     gene            complement(6410..6985)
                     /locus_tag="VF752_00655"
     CDS             complement(6410..6985)
                     /locus_tag="VF752_00655"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX8854082.1"
                     /translation="MGFSDAVFAITVTLLVLEIRPPTDDRNLLHGLLALWPSYLAYAV
                     TFLFIGQVWANHHVMFDHITSADRVVLLLNTLLLMVVAFLPFATSVLAGSVRSGHGQR
                     TAVVFYAIAFDVTALTFNAVWQYARRHRLLSEALDPAGATNISRRFQLALAWLSTGAL
                     LGVVLPVLGLAVIAAFNAFYWLPIRGESPHS"
     gene            complement(7125..7358)
                     /locus_tag="VF752_00660"
     CDS             complement(7125..7358)
                     /locus_tag="VF752_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854083.1"
                     /translation="MADLRRLDEFLRAEAGDAGCTAGEDILDAYIELELAGEDPARVF
                     PGTAIHLRSCPGCRADHNGLLEAARRFADAQPE"
     gene            complement(7358..7981)
                     /locus_tag="VF752_00665"
     CDS             complement(7358..7981)
                     /locus_tag="VF752_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8854084.1"
                     /translation="MPQDRECEQWVKQLHPGHPRRDEAVASLRVVLLRVAYHELSRRR
                     GQLASIMGAEFDDLAQQAANDALINVLARLDDFRGLSRFTTWAYKFVMFEVSGKVARH
                     IWRGQSPSREGLAFEQLPDLLAPQPGDRIEQREQLKALAVAIGELTDRQRQVFVAIAL
                     NEVPIDVLAVQLGTNRNAIYKNLFDARRNLRASMAAAGHPVPEEDAA"
     gene            8184..8900
                     /locus_tag="VF752_00670"
     CDS             8184..8900
                     /locus_tag="VF752_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8854085.1"
                     /translation="MDDGGPRFDDLRALFVNCTLKRSPEPSNTDGLIELSSGIMRRRG
                     VVVDALRAADHDIATGVWPDMTEHGWAVDEWPAISERVLAADILVLCGPIWLGDNSSE
                     MKRVIERLYASSTLLNARGQYAYYGRAAGCLITGNEDGVKHCAMNVLYSLQHLGYTIP
                     PQADAGWIGSIGPGPSYLDPDSGGPENDFTNRNTSFMTFNLMHVAAMLKRSGGLPAFG
                     NQRTEWDAGCRPDFANPEHR"
     gene            8926..9579
                     /locus_tag="VF752_00675"
     CDS             8926..9579
                     /locus_tag="VF752_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX8854086.1"
                     /translation="MTTGPDTVPDAVLDRVACASLVDAMGRLHSHRAHILSLVSPAPR
                     RLFGPVATIAYLPYRDDLRQTELGFGALFHQAVGESAAGTVLVLSSGGYPEASHGGGT
                     KLSRLQHTGAAGVLADGRLRDFEQLRGYAFATWCRGEATRWGGDTVMPFAANVAVEIR
                     GVCVIPGDYLYADASGAVVIPRASLKQVIDEALRVEAEDATSLEEIATERLEGTSRD"
     gene            complement(9603..11363)
                     /locus_tag="VF752_00680"
     CDS             complement(9603..11363)
                     /locus_tag="VF752_00680"
                     /EC_number="2.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="HEX8854087.1"
                     /translation="MSSPVGALLSVNVGMPKDVPWQGKTVFTGVFKEPVTGPRRVRKL
                     NVDGDGQGDLAGHGGEQRAVFVYQIDSYRYWERELGRNDFVYGQFGENFTIEGLSDDE
                     VCIGDRYQIGSAVFEVTQPRVTCYRVGIRMNDPRIPALLVSHHRPGFYFRVLQEGDVQ
                     AGDQILKLASGPEQMTVAEVNALLYLPGHPRQQLLRALRIPALSPGWQASFRGLLDGE
                     PGGGNAGLSTASPPPAWPGFRRLTVTGIERESDSVISIRLEDPGGAPVPPARPGQYLT
                     LRIQPEKEQRSVLRNYSLSGPPDAGYYRITVKREPEGVASGYLHTRLAVGDQLDIAAP
                     RGTFILDQTSAPVLLISAGIGATPVLAMLQALATEHAQREVWWLHGARSGREHSFAAE
                     TRALLRSLPNVRIHVYYSRPDTNDLEGRDFDSAGRLTGSLLAALGPPPNAEAYLCGPA
                     PFMDEISAGLAAIGLDASRIHTEPFGPAPGLTPGIAATAARTPHPPAGHPGTGPTIEF
                     ARSDLAVPWSDDYSSLLELAEACDVPVRWSCRTGVCHTCETTLTAGEVDYSPDPVEPP
                     ADGSVLICCSKPRDNVVVDL"
     gene            complement(11366..12259)
                     /locus_tag="VF752_00685"
     CDS             complement(11366..12259)
                     /locus_tag="VF752_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX8854088.1"
                     /translation="MGLSWQQGPLGRDPNGTFLTQSPMPERVLYVEPLRRRMSVELGG
                     SIVARSDDAVLLFEPARYPVAYFPIGDIAGGVLLPTDRETAHPDLGKTRWFDVVGGDG
                     QIAKRGAWQHVDPPPQAAALRDTVAFAWRGMDAFYEEDERIFGHAADPYHRIDIRRSS
                     RHLVVRQDDQVVADTHAPLVLYESGFAPRWYVPRGDVVANALRPVEGQTFCPYKGLAS
                     YYDIGNARNAAWSYRVPFEEMARIADLVSFEPDKVTITIDGEKLEPAAGQTVLPHGPD
                     RNLSVDEVGGIHVVEPATSGA"
     gene            complement(12536..12976)
                     /locus_tag="VF752_00690"
     CDS             complement(12536..12976)
                     /locus_tag="VF752_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854089.1"
                     /translation="MTTPTFSLEVITLPVSDVDRALRFYVDQVGFTLDVDYAPNVAFR
                     VVQLTPPGSSCSIQIGTGLTDAAPGSVRNAYLVVSDLESARSRLLERGVEVSEIRHKT
                     PIGDWDGGFAPGLDPAHGDYASFAGFSDPDGNSWTLQERGHAGA"
BASE COUNT         2051 a         4431 c         4612 g         2221 t
ORIGIN      
        1 ccgcacgcgc ggaggatccg gtggggctgg ccgtggcgcg ggccaatgcc gaggccgccg
       61 cgctgctgcc ggtcgtgcgc gacacggctc gacgttctgc tgcgccgcca cctgatcgcg
      121 gcgcggcgca ccctcctcgg cgaggcggca gacaccggct acgagacgca gcggctggcc
      181 gttggcttcg tagacctggt gggatcaacg gcgctcgccc agcaactctc cacacgcgag
      241 ctcggcgccg tcctgaccga attcgagacc ctggccgccg acatcgtcgc ggcggccggc
      301 ggccgcgtgg tgaagctgat cggtgacgag gtgctctaca ccgccgcgga cccacgctcc
      361 gcctgccgga tcgccgtcga gatgaccagc cgcttagcag accacccgcg agtgccgccc
      421 gcgcgcgcgg gcgtggccag cggcgaggtg ctgctgcgcg acggggacgt gttcggaccc
      481 acggtcaatc tcgccgcgcg cgcggtgaag gtggcccggc ccggcgaggt cgtgatccct
      541 gccgccctgg ctggcgaagc cggcatcacc gcgcgacgct tggggagcat gacgctgcgc
      601 gggttcccgg cggaggtaga gctggcccgt gtggcacctt aggcggcgtt gtgggaatcc
      661 ccaatggacc acacgctagc tttggaccgg tggccgatgc attcatagac ggtggctggg
      721 atcctgccac ccaatgacga tgctgatcgc tcaggtcggc ggctctagcc ggtgacgctg
      781 aacgtaggga tcgggctcgc gctgatttgc gcggccctga cgcagttggg attcctctgc
      841 aagcaccgcg gggcgaacgc cgtgccggcg atcgcgctcg gacgcccgct gcagagcgcg
      901 cgtgcgctgt tacggtcgcg ctggttcgcg atcggattgg gtgtgacagc cggcgcctgg
      961 ctgatgcatg tcgccgcgct cgccatcgcg ccactctcct tcgtccaggc agtgctctcc
     1021 acgggcgtgg tgctcttggc ggtgctgggc gcgtcgctct tcggctgcaa ggtctcccgc
     1081 cggcaatggc tcggggtggc catgaccgcg atcggcctcg tgctgcttgt gctgactctc
     1141 ccgcaccgcg gcggctcgca ctccgcctac gcgtggcctg cgctgttggc cttccagggc
     1201 ggcatgctcg tcgtcggggc gctcttgatc gcggcaccgc ggctgggagc gccgtcccac
     1261 caccacggca ccgctctcgg cgccgccgcc ggcatcctgt tcggcgtctc ggacgtggcg
     1321 gtgaaggcgc tcaccggtct cgccggtcac ggcgcactgg cggttgtcgc gtcaccctgg
     1381 ctgatcgtcg ccgggaccgc ggccgtcctc gcctttctgg cctctgcgcg tggcttccag
     1441 cagggtgacg ccgtgcccgt gatcgcctgc acctccacgg cggcgaacgt cacgtgcatc
     1501 gtcggcggga tcgtggtctt cggagacgcg ctcgctagcc acacgctgct gatcatcgtc
     1561 caggtcattg cgttcgcgct ggtcgccgtg gccgcgatgc tcacgccgac ggcgcacggc
     1621 cgggcccctt cgcaggccgc cgcgtaagcg gtagattcgc gctagggccg cctcgcggcg
     1681 gtcggcgaca cgtctggagg attcggtggc ggtaccgaca gcgcccggcg cagaagccgc
     1741 agctgcgcgc gtcgtcgggc gcctcgtcgc cgacatcgag ggcgagctcg acgctctggt
     1801 cgcccggatc gtcgagcgca tccgtgacga gatccccgac ttccgccgcc tgccagtcga
     1861 agcactgcag agggcggtgc gcggcaacgt cgcgcgggcc ctggccgcgc ttcgcgacct
     1921 gcgaccgccc accccggaag agctggagcg cgcggccgag atcgggcgcg agcgcgccga
     1981 gcaggggctc acggtcgacg cggtcctgca cgcctatcgg atctccgtga cggcggtgtg
     2041 gtcgcgcttc ggcgagctgg ctcgcgagcg cggcgccgac gtggcgagcg tgctcgcttt
     2101 ctctgagacg ctgtggctct gggcggacgc cgtgatggac atcgtcggcg ccgcccaccg
     2161 ccaggtggag ctgcagctcg cgcgcgagga gcaacagcat cgcgacgcgt tcgtgctcgc
     2221 cctcctgatg gggacgatcg acgccgccga cctgaggcgc gacagtgccg cgttcggcct
     2281 cgaccccgag tgcgactaca tcccgttccg cgcccgttcg actcacggcg agggcgctcc
     2341 gccacttccg agccgcgtgg cgctcgcgct ggcgggcgac cgcggcctcg tgaccgcgct
     2401 cgaccacgac ctcatcggca tcgcagcgcg tccgcccgtt cccatccccg aggtggccgc
     2461 aggaatcggg ccgcccgcgc ggctcgcagc tctgcccggc gccttcgcgc tcgcctcgcg
     2521 cgcgctgcag accgcgctcg ccttcgggca ggagggggtg ttcgcgctct ccgacctgtc
     2581 gatccggccc gcgatcctcg ccgacgacgc gctgggcgac gccttcgtaa gccgctacct
     2641 cgagccgctc ggggcgctcg ggcgcctcgg cgcagatctg gaggcgacgc tccgcgcctg
     2701 gttcgagcac ggcatgcgca tcgacgacac ggcccgccgg cttcacatgc acccgaacac
     2761 gcttcgccat cgcctcaatc ggttcgagga ggcgacgggc gcgaacctcc gccacccgac
     2821 cgatctgatc gagctgtggt gggcgctcga gcggcgccgg ctcggagccg aaagccgcta
     2881 gggactcagc ggaactgctg ctcttcggtc gagcccgcga gcgcagcggt agacgtatcg
     2941 ccgccggtga tcgcctgcgc cacctcgtcg aagtatccgg cgccgacttc gcgctggtgc
     3001 tgcgtcgccg tgaagccttc tctctcgctc gcgaactcac cgtcctggaa gtctgcgtag
     3061 gccgtcatgc cgctctcgcg gtagcggcgt gcgaggtcga acatcgagtg ggccagcgtg
     3121 tggaagccgg cgagcgtgac gaactggaac ttgtagccga ggccgccgag ttcgtcctgg
     3181 aacgacgcga tcccctccgc gccgagcttc gccttccagt tgaacgacgg cgaacagttg
     3241 tacgcgagca gcttgccagg gtgctcggcg tgtacggctc gcgcgaactc gcgcgcctcg
     3301 ccgaggtcgg gcgtcgaggt ctcgcaccag agcaggtcgg cgtagggcgc gtacgcgcgc
     3361 gctcgagcca ccgcctggtc catccccgag cgcgtccgga agaagccttc ctcggtgcgc
     3421 tcgcccgtga tgaactcgtg atcggcggga tcgccgtcgc tcaggaccat gtcggcgtcg
     3481 agggcgtcgg tgcgggcgac gattagcgtc ggcacgccga gcacgtcggc ggcgaggcgc
     3541 gccgcgatca ggttgcgcac ggcgtgcgat gtcggtagca gcaccttccc gcccatgtgc
     3601 ccgcacttct tctccgaggc gagctggtcc tcgtagtgca cgcccgcggc gcccgcgtcg
     3661 atcatcgcct tcgtcaactc gaacacgttg agcgcaccgc cgaaacccgc ctcaccgtcg
     3721 gcaacgatcg gggcgaacca gtcgatcccg tcgttcccct cggcgtgctc gatctcatcg
     3781 gcccgctgga gcgcgtggtt gatccgccgc acgatctcgg gcacgctgtt cgccgggtag
     3841 aggctctggt cggggtacat ctcccccgcg aggttcgcgt ccgctgcgac ctgccagccg
     3901 ctcacgtaga tcgccggcag acccgccttc acttcctgca cggcctgctg gccggtgagc
     3961 gcgccgagcg ttcgcacggg ctcgtcacgc ccgagcatgt cccacaggcg ctcggctcct
     4021 atgcgggcga gcgtgtactc gatgtggacg gttccccgca gccgctccac atcctcgggg
     4081 gcgtacggcc gcttgatccc gtcccagcgc ccggggacat cgtggcttcc atttcccgct
     4141 cgagacgtca tggcagccgt tttcccgagc ggcggcggtg gaaactccga cacttcgact
     4201 aatcgacggg tcccgagtcc ttcaggtgcg cggaccagcg ctcctctatc cgcccaagcc
     4261 gccagatcag tagcgcgatc acccaggtgg cgacgaacat gacgacgatc accaacccga
     4321 ggaagttgat gtcgacgttc tcaaacgagt tccaaaaggt tccggacagg ccgagctttg
     4381 agctgatcag accgccgacc tcgatcgtgc cgatcacgag cgcgaccgcc acagataggc
     4441 ccgtgatcgt gaggttgtag aacaccttgc gaaccggctt ggagagcgcc cacccatacg
     4501 cgaagttcat gaaggacccg tcgatcgagt ccatcagtga catccccgcg gcgaacagga
     4561 tcggcaggca gaggatcgcg taccacggca acccggccgc ccctgcgccg ccggcgatga
     4621 ccaggagcgc cacctcgctc gccgtgtcaa acccgagccc gaacaacaac ccgaccggat
     4681 acatctgccg cggcttggcc acggccttgc tcaggcgtcc gaatagccgg ttcatcaatc
     4741 cgcggctgtc cagctgccgc tcgagcccgg tttcgtcgaa gcgcccgctc ctcatctcgc
     4801 ggaacacctt cacgatgccg agcaggatca cgatgttgat cgccgcgatc aggtagagaa
     4861 accccccgga caccgcggtg ccgacagtgc tcgtggtctg gcgcaacgac gagtgaccgt
     4921 cctggacggg cccgtcgagc gagcgcacgc cgatcgaaat caagagcaca agtgcgaaga
     4981 cgactgtcga gtgtccgagt gaaaaccaga aaccgacgct caacggccgc ttgccttcgg
     5041 ccatcaactt gcgtgtggtg ttgtcgatcg cggagatgtg atcggcatcg aacgcgtgtc
     5101 gcagcccgag cgtgtaagca gtgaccccaa tcccgatcgg cagtaccggg gccgtgccga
     5161 ggtggtaagg ccttggcgct gagaacgcga ccagcgcgac gaagccgagc acgtgcaagc
     5221 cggcaaccgc cgcgaccatc gccgccagcg tccgccgctc gtgacggctc agcgatgccc
     5281 agaacactgc gcggtgtgtg tggtgtgtgt gtctacgcgg cgcgcttcgt ggtcacgacg
     5341 tgagcaacgc tcctccgccc gtgtcctggt caggcgcttc cgcgttcggt gggccgagga
     5401 acgggaactc ggcgaggtag tcgtcgtgcg gcccgacgcc atcggaggtc acacgtccgt
     5461 acgtgaggaa tgccatgcgc gtgtcgtaga cgtcgtcggt cagagcccgg ccgttggggt
     5521 agtgcgccgg ccgattgcgg tcgtagtgga ggatgtcggg gagcacggtg aggacggtgt
     5581 ctctggcctc ggcgggcggg tagccgcgac tctccagtag ccgcgaccag ggctccaggt
     5641 agtccgccac gtcgttcgcc gggtgtccgg cgttgaactc gttcttgagc tcgtcaacga
     5701 agaacgggtt gaacgacggg ttcccgattc ggtcgacctg gacgagcttg ccgccgcggc
     5761 gcacactcac tgtgccccaa agtccgacct ccggaccggg gccgagcatg tcgttgggca
     5821 cctccagcgc catgctgagg atgttcttgc cggcgaacgt gtcgtcgccg gtgaactgaa
     5881 agtcgtggaa tgcgccttcg ccgtcggcga agaacggatc gctgcggacg ccgatgaaca
     5941 gccgacacga accggcttcg accggcgtcg ccatggtgtc gaagctgacg ggcgtgccct
     6001 cgatgagcac ctcgccaacg ggctcggcct cccgagcctg gcttcctgtc gcataacgca
     6061 ccgttccggt ctggctgccg ccgttcgact cggagaacac aaatgagaag gcagcgtcgg
     6121 cctgcgtgtc tccgtcgttg tcgacgttga tgcggtagac cgcatcggga tgaaaatcgg
     6181 cgcccgtcat gaatggattc acgtcgatga tcagcacggt cttgcccgga ctctctgccg
     6241 actcgaaaac gaagagatcg gtcaagtcca ggcgcgcatc gtcacccggg aacttgagca
     6301 tcgcggcact gaagtggttg gacatcgtgg atcctttgat tgctggttag ggcatcgctc
     6361 acgtgggata ggtgtgcgac gggcaaccgc gtcttaccgc cgctcaggct caggagtggg
     6421 gactctcgcc acggatcggg agccagtaga aggcgttgaa cgcggcgatc acggccaagc
     6481 ccaggacggg cagcaccacg ccaagcagtg cgccggtcga gagccaggcc agggcgagct
     6541 ggaagcgcct gctgatgttt gtcgcgccgg cagggtcaag agcttcgctg agcagccggt
     6601 gacggcgcgc gtactgccag acggcgttga acgtgagtgc cgtcacgtcg aacgcgatgg
     6661 cgtagaagac gacggcggtt cgctgaccgt gtccgctgcg caccgacccg gcgaggacgg
     6721 acgtggcgaa cgggaggaac gcgacgacca tcagcagcag ggtgttcagg agcagtacga
     6781 cccggtcggc cgaagtgatg tggtcgaaca tgacgtggtg gttggcccac acctgtccga
     6841 tgaacaggaa tgtgacggcg taggcgagat aggacggcca gagcgcgagc aggccgtgca
     6901 gcaagttcct gtcgtcggtc ggcggtctga tctccagcac gagcagggtg acggtgatcg
     6961 cgaacaccgc atcgctgaac cccaccgacc gaatcgtgtc gcggtgcacc gacgctgggt
     7021 cggcccccgc ccccttgcct ccaggcgctc gcgccatcgc ctgggatagt gcgcggacgc
     7081 aggcgcgtct taccggcccg cctcgccttc aatgctcggg gcgcctactc gggctgggcg
     7141 tcggcaaacc ggcgggccgc ttcgagcaag ccgttgtgat cggcacggca gccggggcag
     7201 ctccgaaggt ggatcgccgt gccggggaac acgcgtgccg ggtcttcccc ggcgagctcg
     7261 agctcgatat aggcgtcgag aatgtcctcg ccggcggtgc agcccgcatc cccggcctcg
     7321 gcgcgcagga actcgtcgag gcgacgaaga tcggccatca ggctgcgtcc tcctcgggca
     7381 caggatgtcc ggcggcggcc atgctggcac gcaggttacg cctggcgtcg aagaggttct
     7441 tgtagatcgc gttgcggttg gtgccgagct ggaccgctag gacatcgatg ggcacctcat
     7501 tgagcgcgat cgcgacgaac acttgccgct gccggtcggt gagctcgccg atcgcgaccg
     7561 ccagggcctt gagctgctct cgctgctcga tccggtcgcc gggctgaggc gcaagcaagt
     7621 cgggcagctg ctcgaatgca agcccttctc ggctcgggga ctgtccgcgc cagatgtggc
     7681 gcgccacctt gccggagacc tcgaacatca cgaacttgta cgcccaagtg gtgaagcggc
     7741 ttaatccgcg gaagtcgtcg agcctggcga ggacgttgat gagcgcgtcg ttcgcggctt
     7801 gctgggcgag atcgtcgaac tcggctccca tgattgacgc cagttgaccc cgtcgccgcg
     7861 agagttcgtg gtaggcgacg cgcagcagta ccacgcggag cgaggctacc gcctcgtcac
     7921 ggcgcggatg tccggggtgc agctgtttga cccactgttc gcactcgcga tcctgaggca
     7981 ttcggctcgg ttggttgccg ctggtcggcg ctggctcacc actcaccgtg tggccccccg
     8041 ctgatggcga tgctcctcag atggactgcc atcacggctg cgcatcgggc ggattatcgc
     8101 gcccgcaggt ttcctgcagg taagaactct ccgccggccg accctaatcc gattgacagc
     8161 gagagcccca ctggagggac aagatggacg atggcggtcc acgcttcgac gacttgagag
     8221 ccctattcgt caactgcacg ctgaagcgtt ctccggagcc cagtaacacc gacggtctga
     8281 tcgagctgag cagcgggatc atgcgccgcc gcggcgtcgt ggttgacgcg ctgcgtgcag
     8341 ccgatcacga catcgccacg ggcgtgtggc cggacatgac cgagcacggc tgggcggtcg
     8401 atgagtggcc ggccatttcc gagcgggtcc ttgctgcgga catcctggtg ctgtgcggtc
     8461 cgatctggct gggcgacaac agctcggaga tgaagcgggt gatcgagcga ctctatgcgt
     8521 cctccacgct gctcaatgcg cgtggtcagt acgcctatta cggccgcgcg gccggatgtc
     8581 tgattacagg caatgaggac ggcgtgaagc actgcgcgat gaatgtgctt tacagcctcc
     8641 agcacctcgg ctacaccatc cctccacagg ccgacgctgg gtggatcgga tcgatcggcc
     8701 ccgggccgtc ctacctcgac cccgactcgg gcggcccgga aaacgacttc accaaccgca
     8761 acacgtcgtt catgaccttc aacctcatgc atgtggccgc gatgctcaag cgcagcggcg
     8821 gcttgcccgc gttcggcaat cagcgcactg agtgggatgc ggggtgccga ccggacttcg
     8881 cgaacccgga gcaccgatga gccaggccgt gataacgaca cggcgatgac caccggtccc
     8941 gacaccgtgc ccgacgccgt gctcgatcgc gtggcgtgcg cgtccctggt tgacgccatg
     9001 ggtcgacttc acagccatcg tgcgcacatc ctcagccttg tcagccccgc cccgcggcga
     9061 ctgttcgggc cggtggccac gatcgcctat ctcccgtatc gcgacgatct tcggcagacc
     9121 gagcttgggt tcggcgcctt gtttcaccag gcggtcggcg agtcggcagc gggcacggtg
     9181 ctggtgctct ccagtggcgg ctaccccgaa gcctcccacg gcggcgggac gaagctctcc
     9241 cgcctccagc acaccggggc cgccggggtc ttggcggatg ggcggctgcg cgacttcgag
     9301 cagctacgcg ggtatgcgtt tgcgacctgg tgccggggcg aggccacgcg ctggggtggc
     9361 gacacggtca tgcctttcgc cgccaatgtc gcggtggaga tccgcggcgt ctgcgtgatc
     9421 cccggcgatt acctgtacgc ggacgccagt ggcgcggtcg tcattccccg agcaagcttg
     9481 aaacaggtga tcgacgaagc gctgagggtc gaagctgagg acgcgacgtc gctcgaggaa
     9541 atcgccaccg agcgactcga ggggacatcg cgcgactgag gatcaccgag cgctgcgccg
     9601 agtcagagat caacgacgac gttgtccctc ggctttgagc agcagatgag tacgctgccg
     9661 tcggcgggcg gctcgacggg gtcggggctg tagtcgacct cgcccgctgt gagcgttgtc
     9721 tcgcaggtgt ggcagacgcc ggtgcggcac gaccagcgga cgggtacgtc gcaggcttcg
     9781 gcgagttcga ggaggctgct gtagtcgtcg ctccacggca ccgcgaggtc gcttcgggcg
     9841 aactcgatcg tcgggccggt tccggggtgg ccggccggcg ggtggggcgt ccgcgccgcc
     9901 gttgctgcga tgccgggcgt gaggcccggt gcgggtccga acggctcggt gtggatgcgt
     9961 gaggcgtcga ggccgatcgc cgccaagccc gcgctgatct catccatgaa tggggctggt
    10021 ccgcagaggt atgcctcggc gtttggcggt ggcccgagcg cggcgagcag cgacccggtg
    10081 aggcgcccgg cgctgtcgaa gtcgcggcct tcgagatcgt tcgtatcggg ccggctgtag
    10141 tagacatgga tgcgcacgtt cggaagggaa cggaggagcg cccgggtctc ggcggcgaaa
    10201 gagtgttcgc ggccgctacg cgcgccatgg agccaccaga cttctcgctg ggcgtgctcg
    10261 gtggcgagtg cttgaagcat ggcgagcacc ggggtcgcgc cgatgccggc gctgatcagc
    10321 agtaccggcg cgctggtttg gtcgagaatg aaggtgccgc ggggtgctgc gatgtcgagc
    10381 tggtcgccca ctgcgaggcg ggtgtggaga tagccgctgg cgacgccctc gggttcgcgc
    10441 ttgacggtga tcctgtagta gccggcgtcg ggcggtccgg acagggagta gttccgaagc
    10501 accgacctct gctccttctc gggctggata cgaagcgtga ggtactggcc gggacgcgcg
    10561 ggagggaccg gtgcgccgcc cgggtcctcg agccgtatgg agatcacgga gtcgctctcc
    10621 cgctcgattc cggtcactgt cagccgccgg aatcccggcc aggcgggcgg cgggctggcg
    10681 gtgctcaggc cggcattgcc gccgccgggt tctccgtcga gcaggccgcg aaatgacgct
    10741 tgccagccgg ggctgagcgc gggaatgcgc agcgcgcgga gcagctgctg gcgcgggtgc
    10801 cccggcaggt agaggagtgc gttgacctcg gcgaccgtca tctgttccgg gccggaggcg
    10861 agcttgagga tctggtctcc cgcttgtacg tcgccttctt ggagcacgcg gaagtagaag
    10921 ccgggacggt ggtgtgagac gagcagcgcc ggtatgcgcg ggtcgttcat ccgaatcccg
    10981 acgcggtagc aggtgacgcg cggttgcgtc acttcgaaga cggcgctccc gatctggtac
    11041 cggtcgccga tgcagacctc gtcgtcgctc agtccttcga tggtgaagtt ctcgccgaac
    11101 tgtccgtaga cgaagtcgtt tcgtccaagc tcgcgttccc agtaccgata ggagtcgatc
    11161 tggtagacga acacagcgcg ttgctcgccg ccgtgcccgg ccagatcgcc ctggccgtcg
    11221 ccgtccacgt tgagcttgcg cacacggcgg ggcccggtga cgggctcctt gaacacaccg
    11281 gtgaagacgg tcttcccttg ccacggcaca tccttcggca tgccgacgtt gaccgacagc
    11341 aaggcgccca ccggcgagct catgctcacg cgcctgacgt cgccggttcg acgacgtgga
    11401 tgccgccgac ttcgtcgacg ctgaggttgc ggtcgggacc gtggggaagg acggtttgcc
    11461 cggcggcggg ttcgagcttc tcgccatcga tcgtgatcgt caccttgtcg ggttcgaacg
    11521 agacgaggtc ggcgatacga gccatctcct cgaacgggac ccggtacgac caggcggcgt
    11581 tgcgcgcgtt cccaatgtcg tagtaggacg cgagaccctt gtaagggcag aaggtctgcc
    11641 cctcgacggg acgaagggcg ttggcgacga cgtcgccgcg aggcacgtac cagcgcgggg
    11701 cgaagcccga ctcgtagagc accagcggtg cgtgggtatc tgcgacaact tgatcgtcct
    11761 gccgcacgac gaggtggcgc gagctgcgcc ggatgtcgat ccggtgatag gggtcggcgg
    11821 cgtgcccgaa aatccgttcg tcctcttcgt agaaggcgtc cattccccgc catgcgaacg
    11881 cgaccgtgtc gcgcaacgca gcagcctgcg gcggcggatc gacgtgctgc caagcgccac
    11941 gcttcgcgat ctggccgtcg ccaccgacga cgtcgaacca tcttgtcttg ccgagatctg
    12001 ggtgtgctgt ctctcggtcc gtgggcagca ataccccgcc tgcaatgtct ccgatcggga
    12061 agtaggcaac tgggtagcgg gccggctcga agagaagcac cgcgtcgtcg ctccgggcga
    12121 cgatgcttcc gccgagctca acgctcatcc tccggcggag cggctcgacg tagagcacac
    12181 gctcgggcat cggcgactgt gtgaggaagg ttccgttggg atcgcgcccc agcggcccct
    12241 gctgccatga aagtcccatg actgctccga tcgtccgtgg gctatctgtc agttttggtt
    12301 gtcgcgtcca ccgccccgct cttaccgagg ctgagcgcga ggagcggagc cggctccccg
    12361 cgacccgcag ccgcggcggc gaagccgtcg gggctgacga gccagctgcg gagccgcctc
    12421 ataaaggacc tgttgttctg atggcggctc gacattgcgc acgctccttc cggtgggtgt
    12481 ggcttagaca gtgcgcggcg ggcgccggtt cttaccgggc atcagccgtc cacgatcacg
    12541 ccccggcgtg gccccgttcc tgaagggtcc agctgttgcc gtcgggatcc gagaagccgg
    12601 caaagctggc gtagtccccg tgcgccgggt cgaggccagg cgcgaatccc ccgtcccagt
    12661 caccgatcgg cgtcttgtgc cgtatctcgc tgacctcgac accgcgttcg agcaggcggc
    12721 tccgtgcgct ctcgaggtcg ctaacgacga gataggcgtt gcggaccgat ccgggggccg
    12781 cgtcggtgag cccggtgccg atctggatcg agcagctcga ccccgggggc gtcagctgca
    12841 cgacccggaa cgcgacgttc ggcgcatagt cgacgtcgag cgtgaagccg acctggtcga
    12901 catagaaccg caacgcgcga tcgacgtcgc tcaccggcag cgtaatcacc tcgagtgaaa
    12961 acgttggcgt tgtcacaact gattcgtgcc aagactgaca ccggtcttac cgtcagccag
    13021 cacgacgcgt cgcgacagtg cgttgagcag taagaaccgg ccgcggggcc actctcatgt
    13081 tgccgaacgg gccaatccac acgggcctaa tcggaaggaa gaagacatga ccgcaaattc
    13141 gaacgcgggc acgatcgacc ggcaagcatg acttcgactc agaacacggc tgcctgtgct
    13201 cgcggcggtc tcgctgtggt gcagtccagg gaacccgctg ccccagaagg agacgaaccc
    13261 gcttcctcgg cggtgcgtgt cctggtggcc gacggtgagc agctggttcg cgccg
//
LOCUS       DASVXS010000014        13624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1135958, whole genome shotgun sequence.
ACCESSION   DASVXS010000014 DASVXS010000000
VERSION     DASVXS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135958"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VF752_00695"
     CDS             <1..697
                     /locus_tag="VF752_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854090.1"
                     /translation="VETAESYYDEVSTTVRYEGRIRTIEAVKEAGLETCVGGILNLGE
                     SREQRVEMAFQLASINPTSVPINLLNPRPGTKFGDRELIDPWEAVKWIAIFRLILPDA
                     LFRLCGGRVENLGELEPLAVKAGLNGVMMGNFLTTLGSEPEDDRRMFEQLGLNTARQP
                     DNGSNPRPDNRSGWLEGESPARAEDGLVDRQDEAPFWDPATQLRHRKKTSVPPRPDGA
                     ANTYPTVIELPAA"
     gene            710..1927
                     /gene="bioF"
                     /locus_tag="VF752_00700"
     CDS             710..1927
                     /gene="bioF"
                     /locus_tag="VF752_00700"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX8854091.1"
                     /translation="MNEIAERLDEIKSRGLHRRMRMVSGPQGPRVLLDGKPVLLLCSN
                     NYLGLADHPRVREAAADAAMRWGVGSGASRLVSGNMTIHRRLEDKLAAFKRTESCLLF
                     GSGYLANTGIVAALASEGDVVFSDELNHASIVDGCRLAKAETFVYDHCDMEHLEWGLQ
                     QAAGRGSLIVTDGVFSMDGDIAPLTEIVELAGRYDTRVMVDDAHGTGALGPGGRGSVA
                     AAGLEHEIDVLVGTLGKSLGSYGAYACCDSEMAEYLVNCARTLIFSTALPPPAVAGAM
                     AALDLLIAQPERVDKLQRNGAALRAALADEGLDTGGSETQIVPLVVGAADDAMSACEK
                     ALKRGVFAQAIRPPTVPSDTSRLRLAVMASHTATELRGAAKVLAQSLPDVVREAAPKQ
                     ERPRVFDGWAEAA"
     gene            1936..2610
                     /gene="bioD"
                     /locus_tag="VF752_00705"
     CDS             1936..2610
                     /gene="bioD"
                     /locus_tag="VF752_00705"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX8854092.1"
                     /translation="MRGLFVAGTDTGAGKTVLAAAICAALAARGERVAAFKPIVTGLD
                     EQVDEWPRDHELLAATASAGQMAADVATRSFGPPVSPHFAAQLADEMLDPAALVEAAR
                     AAAEGADALVCEGVGGLLVPLTPEYLIRDFAADLGLPVVIASRPALGTINHSLLTVEA
                     ARARGLDVRGIVMTPWPAEPDGMLVSNRETVERLARVEVATLRETSPGALAEAGAALP
                     LDRWLD"
     gene            complement(2607..4268)
                     /locus_tag="VF752_00710"
     CDS             complement(2607..4268)
                     /locus_tag="VF752_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8854093.1"
                     /translation="MSEKGVEMSYRKYRLGSYEEMVAAHRWDVPERYNIASDVCDKHP
                     PDKLAMIWEDWRGQERSVTFGELQGLSNRFANALEAHGVERGDRVATLLPSLPETAAV
                     FLGIYKRGAILLSMSVLYGDEGIAHRINDSGAKAIVTDTKNRHRIPEGVDVVFVLDTG
                     EGERQAGDVVFSEALDAASDRYDVADTAADDPAQLYYSSGTTGLAKGILHAHRYLLGH
                     EEFEFCHDVQENELFHGMGEWAWAAGICPLLGPWRYGATQLVLARKAGFDPEEQLRFL
                     SKHGVQNMFTTPTALRAMRGVDNAGQRYPLDQLRVTCSAGEPLNPEVIHWFRDQYGLT
                     VLDYYGLTESYPLCGNYPTVEVREGSMGLPMPGWDVAILDEDEKPLPPGERGEICLKA
                     RTNPHYPLGYWNRPEDSEEVFGGEWFHTKDAAQTDEDGYVWYAGRADDVIISAGYRIG
                     PFEVESACVEHPAVLEAAAVASPDELRGTIVKAFIVLTEGKSPSDDLASEIKTHVRTQ
                     LSAYAYPREIEFVDDLPKTRTGKIRRIELREAERAKKEAQAASPS"
     gene            complement(4261..5754)
                     /locus_tag="VF752_00715"
     CDS             complement(4261..5754)
                     /locus_tag="VF752_00715"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX8854094.1"
                     /translation="MPPLGLLVFVVGAGSLGAEIAAVRLLAPDFGASTIVWANTIGVV
                     LVALAAGYWLGGRLADRHPHIRGLCMLSLVAAALLALVPFAADPLLDVGVKALDSVSA
                     GAFAGSLLAVLVLVAVPVLLLGGVSPWALRLAVSDVRTAGSVAGRLYALSTAGSLFGT
                     LLSALVLIPLVGTRRTFLVFALSIALVAMLGVWGARRYVLVPAAIASLMAIPVGTLRA
                     NPDGRVIYETETPYQYAQVVQHEDGSRALELNEGQARHSLYRPGTVLTGDYWDEFLVL
                     PFASADRAPRRIAILGNAAGTTARAYGRLHPNTAVDGVEIDGKLSEIGRRYFDMRGPR
                     LNVITQDARPFLRRTRQSYDSIFVDAYRQPYIPFYLATREFFELVEGALRPGGSVIVN
                     VGQPEGSHALERVLAATMRTSFTTVLRDRAQRTNTLLLASDSSASAARLRANAERVHP
                     SVSQLARDAAQRLEPALRNGEIYTDDRAPVEWLVDGSLFDYARGARE"
     gene            5790..7091
                     /gene="bioA"
                     /locus_tag="VF752_00720"
     CDS             5790..7091
                     /gene="bioA"
                     /locus_tag="VF752_00720"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX8854095.1"
                     /translation="MTLASLDRDHLWHPFTQQRGWAEEEQLVIERAEGTDLIDTEGRR
                     YIDGVSSLWCNVHGHRHPKIDEAVRDQLDRVAHSTMLGLSHPPAIELARRLVEIAPPG
                     LSRVFYSDSGSTAAEIALKMAFQYWQQQGGDAAKKTKFVSLREAYHGDTIGSVSVGGI
                     DLFHATYRPLLFDTLKAETGDAADMERLLVENEGEVAAVIVEPLVQGAAGMLLQPDGY
                     LRQVREMCDRHGALLVCDEVAVGFGRTGRMFACEHEDVAPDFLCLAKGITGGYLPLAA
                     TLATERVYEGFLGEHHEFRTFFHGHTYTGNPLACAAALASLDVFEEEDVIGRLQGKIA
                     LLERLLEPIAALPCVSEVRQKGFMVGIELGGFAVEERMGHQVTLAARERGAVIRPLGD
                     VVVLMPPLGIDESELIRLVEITGEAIAAATGIARAEQRQAA"
     gene            7129..7341
                     /locus_tag="VF752_00725"
     CDS             7129..7341
                     /locus_tag="VF752_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854096.1"
                     /translation="MAALLTITPGPEIAVVARSALERGFGPAVWRAISRLTGETPEFG
                     SAYENQSQELDLILRGVRATLVPKRN"
     gene            complement(7396..7575)
                     /locus_tag="VF752_00730"
     CDS             complement(7396..7575)
                     /locus_tag="VF752_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX8854097.1"
                     /translation="METATQQLWICESCGFIYDPAEGDPDGGVPPGTLFQDIPDDWFC
                     PVCGARKRDFVPYEG"
     gene            7615..8232
                     /locus_tag="VF752_00735"
     CDS             7615..8232
                     /locus_tag="VF752_00735"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8854098.1"
                     /translation="MRFDRPPPGEELNPGEVTKAREAASVIVLRDSEAGPEVLLVQRN
                     PEARFMGGAWVFPGGALGKEDAGLEQTALRELDEEAGIALGDSSLLVPFSRWITPAQV
                     KVRFDTLFYVTEAPDGAEPVCDGKECVDLRWIRPADALDAGRRGDLMLVFPTIKHLEQ
                     LAAFESVAHALEVARSRTVEPVQPRVVVNESSAQVLLPGEPGYDD"
     gene            8281..9051
                     /locus_tag="VF752_00740"
     CDS             8281..9051
                     /locus_tag="VF752_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8854099.1"
                     /translation="MRRTVFTATAAVAACLILAAIAFADSFIGSSGDDVLAGTPGPDQ
                     IYGEDGNDTLNGGDANDYLEAGPGDDQVNAGPGGDLVIGGTGSDRVDAGPGPDVAYAG
                     PGSDELLGGDGPDVLFGQIGDDRIFAGSGDDRLYTGRGQDFADGGAGDDRIAADLGDS
                     VLNGGAGRDFIVAVAGGSFTVDGGPGDDRITLGPGNDKVREGGGADRISSGAGDDTID
                     VKDGRRDRVDCGASQDTVVADDKDRLVHCELRSTAANR"
     gene            9065..10090
                     /locus_tag="VF752_00745"
     CDS             9065..10090
                     /locus_tag="VF752_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8854100.1"
                     /translation="MARNGLTVAVTGPTGDLGMAIVSALERSRRVQKIIGMARSPFDP
                     AAAGWRKTEYRQGDVQDRASVRHAVKGADVVVHLAFAVVEASDTTREMNVEGSRIVFE
                     EASAGGADRICYASSVAAYGFHEDNPNWLTEDIPPRGTPEHFYSQQKAEVEEVLGQVL
                     LRRPATTAYVFRPCIVAGPRAQTMLEEIPYLRLSERMPDAVVHLLSFMPILKPVIPDP
                     GVRFQLVHEDDVASAFLAGVLGKGSPGPYNLAANGTLKVPDLADALGWYSIPVPELAV
                     EATAEVIARLPIGPAQLSWIQSARKPVLMKTDRARKELGWRPQHTARATLKELVAAYR
                     GDAERVR"
     gene            complement(10087..10356)
                     /locus_tag="VF752_00750"
     CDS             complement(10087..10356)
                     /locus_tag="VF752_00750"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX8854101.1"
                     /translation="MCRNIRTLHNFDPPATSEEVQAAALQYVRKVSGFTKPSKANEEA
                     FNRAVHEVAHVTAHLLEDLVTNAPPKDREVEAAKRRARAAERYAA"
     gene            complement(10366..10836)
                     /locus_tag="VF752_00755"
     CDS             complement(10366..10836)
                     /locus_tag="VF752_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEX8854102.1"
                     /translation="MIVDAVFDERYWYPEDGGEVWLSGYSIVDPESGEYLARDAPQLA
                     ERGLRTSNVAGAGQHHREALESDGAAPGQPLELRRDPGNEHDPNAIAVLSGSGEQVGW
                     VPRELAAELAPELDAGRPWSAIVLRERRASPRDPRDGITMLLAPADRIELRARG"
     gene            complement(10833..12059)
                     /locus_tag="VF752_00760"
     CDS             complement(10833..12059)
                     /locus_tag="VF752_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8854103.1"
                     /translation="MEAGEAFGARQMTGFKDFYQYNATTRVVAGRGLIEGIGFEYMKE
                     GASRVMVVTDQVIRGTGLVERVEEGLTDGGLEVAGVFDDVPQDSSTDSVARCAEAARE
                     AGADSFLAVGGGSVMDTAKVADVLFAHGGSVRDYEGLYALPREQDGMGSPLPIAPLAC
                     IPTTAGTGSEVSWAAVVKDHEERLKIELVDVPLFPRLALLDPEATRTLPPAVAAASGM
                     DAMTHAIESYVSTEWSPHGDALVLHALRLIRDNLERAVTEPGDEDARGNMLVAASLAI
                     AACGSGAFFGIAHSMSHACGGQYGVPHGMANAINLPHAIRFNAAGDEDIAERYRDVAD
                     VLGVDSRGDVGESLAGHVRSLVDSLGLPTRLSQAGVPEDAVPELAAAAVGDGCTLVNP
                     REVTEEDFAELYRAAL"
     gene            complement(12059..12478)
                     /locus_tag="VF752_00765"
     CDS             complement(12059..12478)
                     /locus_tag="VF752_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8854104.1"
                     /translation="MPYFRDEQEVYDYIGRLFQELVADEELAPKFQKANTTVQYRYRN
                     PESQITVKMVEGEEGQVELGESQLEPEVIMTMEADTAHKFWLGKVNVTVALARGQMKA
                     KGPVAKILKLVPLVKPVFPRYRKMLEDAGREDLVEAA"
     gene            12699..>13624
                     /locus_tag="VF752_00770"
     CDS             12699..>13624
                     /locus_tag="VF752_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEX8854105.1"
                     /translation="MPDSLRRALGAAVLTAAFLAVPAGAFAQAPAEAPTEKVLYKDGY
                     DGRYLLDGTWWFRLDPQDQGLSQGFQRQSSTTGWTQVTVPNAWNATDMSPESQRGTVG
                     WYRKDFRVPRGAKGATWMIRFESVNYRATVFVNGNQVGTHEGAYLPFEVPSGKLRSGV
                     NHLVVRVDSRRSNTDLPPLVDQDNGTPGGGWWNYGGLLREVYLRKVNQVDMTDFLARP
                     LLPCRNCDATVLIRATLHNLGRKKKVRVSARVGSQAARFRPVTLPARGTRQVSAKVKI
                     ANPRLWEPGDPQLYTVRGAVNVGRKVGASWSG"
BASE COUNT         2070 a         4791 c         4722 g         2041 t
ORIGIN      
        1 cgtggagacc gccgagagct actacgacga ggtctccacg accgtgcgct acgagggccg
       61 cattcgcacg atcgaggcgg tcaaggaggc cgggctcgag acctgcgtgg gcggcattct
      121 gaacctcggc gagtcgcgtg agcagcgcgt cgagatggct ttccagcttg cgtcgatcaa
      181 ccccacgagc gtgccgatca acctgctgaa cccccgtccg ggcaccaaat tcggagaccg
      241 cgagctgatc gacccgtggg aggcggtcaa gtggatcgcg atcttccgcc tgatcctccc
      301 agacgccctg ttccggctct gcggcggccg cgtcgagaac ttgggcgagc tcgagccgct
      361 cgcggtcaag gcggggctga acggcgtgat gatgggcaac ttcctcacca cgctcggctc
      421 tgagcccgag gatgaccgcc gcatgttcga gcagctcggc ttgaacaccg cccgccagcc
      481 ggacaacggc tcgaacccga ggcccgacaa ccgctcgggc tggctcgagg gcgagtcgcc
      541 cgcgcgggct gaggatggcc tggtcgaccg tcaggacgag gccccgttct gggatccggc
      601 cactcagctg cgtcatcgga agaagacctc agtcccgccg cggccagacg gcgcggcaaa
      661 cacctatccc acagtcatag aactgccggc cgcgtaggga gggcctcagt tgaacgagat
      721 agcggagcgc ctggacgaga tcaagagccg ggggcttcat cggcgcatgc ggatggtgtc
      781 ggggccgcag gggccgcgcg tgctgctcga cggcaagccg gttctgttgc tctgctcgaa
      841 caactacctg ggccttgcgg accacccgcg tgttcgcgag gcggctgccg acgcggcgat
      901 gcgctggggc gtgggctcgg gggcgtcgcg gctcgtgtcg ggcaacatga cgatccaccg
      961 ccgcctcgag gacaagctcg ccgcgttcaa gcgcacggag tcgtgcctgc tgttcgggtc
     1021 gggctacctc gcgaacacgg gcatcgtggc cgctctcgcg agtgagggcg acgtggtgtt
     1081 ctcggacgag ctgaaccacg cgagcatcgt cgacggctgc aggctcgcga aggctgagac
     1141 gttcgtctac gaccactgcg acatggagca cctcgagtgg ggcctccaac aggccgccgg
     1201 gcgcggcagc ctgatcgtca ccgacggcgt cttctcgatg gacggcgaca tcgcgccgct
     1261 caccgagatc gtggagctgg ccggccgcta cgacacgcga gtgatggtcg atgacgcgca
     1321 cgggactggc gcactcgggc ctggcggccg gggcagcgtc gcggccgcgg ggctcgagca
     1381 cgagatcgac gtcctcgtgg gcacgctcgg gaaatcgctc gggagctatg gcgcctacgc
     1441 atgctgcgac tcggaaatgg ccgagtacct cgtgaactgc gcccgcacgc tgatcttctc
     1501 gaccgccctc ccaccgcccg ccgttgcggg cgcgatggcg gcgctcgacc tgctgatcgc
     1561 gcagccggag cgcgtcgaca agctccagcg caacggcgct gcgctgcgcg ccgcgctcgc
     1621 cgacgagggc ctcgacaccg gcggctcgga gactcagatc gtgccactcg tggtcggcgc
     1681 ggccgacgac gcgatgtcgg cctgcgagaa agcgctcaag cgcggcgtgt tcgcccaggc
     1741 gatcaggccg ccgacggtgc cgtccgacac gtcgcggctc aggctcgcgg tgatggcgtc
     1801 gcacacggcc acggagctgc ggggcgcggc gaaggtgctc gcccagtcgc tcccggacgt
     1861 cgtgcgcgag gcggccccga agcaagagcg cccgcgggtg ttcgacggct gggcggaagc
     1921 cgcctagatc cggcgttgcg cgggctcttc gtcgccggca ccgacaccgg cgccggcaag
     1981 acggtcctcg ccgcggcgat ctgcgctgcg ctggcggccc gcggcgagcg ggtagcggcg
     2041 ttcaagccca tagtcaccgg gctggatgag caggtcgacg aatggccgcg cgaccacgaa
     2101 ctgctcgcgg cgaccgcctc cgccggccag atggccgcgg acgtcgcgac gcgctctttc
     2161 ggcccgccgg tgtccccgca cttcgcggcg cagctcgcag acgagatgct cgatcccgcg
     2221 gcgctcgtcg aggcggcccg cgctgccgcc gaaggcgcgg acgcgctcgt gtgcgaggga
     2281 gtcggcggac tgctcgtgcc gctcacgccc gagtacctga tccgcgactt tgcggccgat
     2341 ctcgggctgc ccgtggtgat cgcctcgcgc ccggccctcg gcaccatcaa ccactcgctg
     2401 ctcacggtcg aggccgcccg cgcgcgaggt ctcgacgtgc gcggcatcgt gatgacgcca
     2461 tggcccgccg aacccgacgg gatgcttgtc tcgaaccgcg agacggtgga gcggctcgcg
     2521 cgcgtggagg tggcgacgct gcgggagacg tcaccgggcg ccctggcgga ggccggcgcg
     2581 gcgttgccgc tcgatcgctg gctcgactag ctcgggctcg ccgcctgcgc ctccttcttc
     2641 gcacgctcgg cctcgcgcag ctcgatccgg cggatcttgc ctgtgcgggt cttcgggagg
     2701 tcgtccacga actcgatctc gcgtggatac gcgtaggccg agagctgagt gcgcacgtgc
     2761 gtcttgatct cggacgcgag gtcgtcgctc ggcgatttgc cctccgtgag cacgatgaac
     2821 gccttcacga tcgtgccgcg cagctcgtca ggtgaggcga ccgccgccgc ctcgagcacc
     2881 gccggatgct cgacgcaggc cgactccacc tcgaacgggc cgatccggta gccggccgag
     2941 atgatcacgt cgtcggccct gccggcatac cagacgtaac cgtcctcgtc cgtctgcgcg
     3001 gcgtccttcg tgtggaacca ctccccgccg aacacctcct cggagtcctc ggggcggttc
     3061 cagtagccaa gcggatagtg cgggttcgtg cgcgccttga ggcagatctc gccgcgctcg
     3121 cccggaggca gcggcttctc gtcctcgtcg aggatcgcga cgtcccagcc cggcatcggc
     3181 aggcccatcg agccctcgcg cacttcgacc gtcgggtagt tcccgcacag cgggtagctc
     3241 tcggtgagcc cgtagtagtc gagcaccgta aggccgtact gatcgcggaa ccagtggatc
     3301 acctcggggt tcagcggctc cccggccgag caggtcacgc gcagctgatc cagcggatag
     3361 cgctgaccgg cgttatccac tccgcgcatc gcgcgcagcg ccgtcggcgt cgtgaacatg
     3421 ttctggaccc cgtgcttcga gaggaagcgc agctgctctt cgggatcgaa gccggccttg
     3481 cgcgcgagga ctagctgcgt ggcgccgtag cgccagggac cgagcagcgg gcagatcccg
     3541 gccgcccagg cccactcgcc catgccgtgg aacagctcgt tctcctgtac gtcgtggcag
     3601 aactcgaact cctcgtgccc gagcaggtag cggtgggcat gaaggatgcc cttcgcgagc
     3661 ccggtcgtac ccgacgagta atagagctgc gccgggtcgt cggcggcggt gtcggcgacg
     3721 tcgtagcggt ccgaggcggc gtcgagcgcc tcgctgaaga cgacgtcgcc cgcctgcctc
     3781 tccccctcgc cggtgtccag tacgaacacc acgtccacgc cctcggggat gcgatggcgg
     3841 ttcttcgtgt cggtcacgat ggccttggcg cccgagtcgt tgatgcggtg cgcgatgccc
     3901 tcgtcgccgt agagcaccga catcgacagc aggatcgcgc cgcgcttgta gatgccgagg
     3961 aacacggcgg cggtctccgg cagcgacggc agcagcgtgg cgacgcgatc gccgcgctcc
     4021 acgccgtgcg cctcgagggc gttcgcgaag cggttcgaaa gcccctggag ctcgccgaag
     4081 gtcacgctgc gctcttgccc gcgccagtcc tcccagatca tcgcgagctt gtccggcggg
     4141 tgcttgtcgc acacgtcgga ggcgatgttg tagcgctcgg gaacgtccca gcggtgcgcc
     4201 gcgaccatct cctcgtagct cccgaggcgg tacttgcggt agctcatctc cactcctttc
     4261 tcactcacga gctcccctcg catagtcgaa cagggagccg tccacgagcc actccaccgg
     4321 cgcccggtcg tcggtgtaga tctcgccatt acgcagggcg ggctcgagcc gctgcgcggc
     4381 atcgcgggcg agctgcgaca ccgacgggtg cacgcgctcc gcgttcgcgc gcaggcgcgc
     4441 cgcggaggcg ctcgagtcgc tcgcgagcaa cagcgtgttg gtgcgctgag cccgatcgcg
     4501 cagcaccgtc gtgaacgacg tgcgcatcgt cgcggccagc acccgttcga gcgcgtgcga
     4561 gccctcgggc tgacccacgt tcacgatcac gcttcctccc ggccgcagcg ccccttcgac
     4621 gagctcgaag aactcgcgcg tcgccagata gaacgggatg tagggctggc ggtaggcgtc
     4681 cacgaagatc gagtcgtagc tctgtcgggt cctacgtagg aacgggcgcg catcctgggt
     4741 gatgacgttc agccgcggcc cgcgcatatc gaagtaacgg cgacctatct cgctcagctt
     4801 gccgtcgatc tcgactccgt cgacggcagt gttgggatga agtcgaccgt aggcgcgcgc
     4861 ggtcgtaccg gcggcgttgc cgaggatcgc gatccgccgg ggcgcgcggt cggcgctcgc
     4921 gaacggcagc acgaggaatt cgtcccagta gtcgccggtg agaaccgtgc caggccggta
     4981 gagcgagtga cgcgcctggc cctcgttcag ctcgagcgct cgcgatccgt cctcgtgctg
     5041 cacgacctgc gcgtactggt agggcgtctc cgtctcgtag ataacgcgtc cgtccgggtt
     5101 cgcccgcagg gtcccgaccg gaatggccat gagcgatgcg atggcggccg gcacaagcac
     5161 gtaccgcctc gccccccata cgccgagcat cgccaccagc gcgatcgaga gcgcgaacac
     5221 caggaacgtg cggcgagtgc cgacgagcgg tatcaagacg agggcggaca gcagcgtgcc
     5281 gaacaggctg ccggccgtgg acagcgcgta caggcgcccg gcaacgctgc ccgccgtgcg
     5341 cacgtccgac accgcgagcc ggagcgccca cggcgacacg ccgccgagca gcagcacggg
     5401 cacggccacc agcacgagca ccgcgagcag cgagcccgcg aaggctcccg cggaaacgct
     5461 gtcgagcgcc ttcacaccga cgtcgagcag cgggtcggcc gcgaagggga ccagcgcgag
     5521 cagcgccgcc gccacgagcg agagcatgca gaggccgcgg atgtgcgggt ggcgatcggc
     5581 caggcggccg cccagccagt agcccgccgc aagtgcaacc agcacgacgc cgatggtgtt
     5641 cgcccacacg atcgtcgagg caccgaagtc gggcgcgaga agccgcacag ccgcgatctc
     5701 agctcccaga gagccggcgc ctacgacgaa caccaggagg ccgagcggcg gcatcgttcc
     5761 gacctggacg atagttacct tggctggccg tgacgctcgc ctcgctcgac cgcgaccacc
     5821 tctggcaccc gttcacccag cagcgcggct gggccgagga ggagcagctg gtgatcgaac
     5881 gcgccgaggg caccgacctc atcgacaccg agggccggcg ctacatcgac ggggtctcgt
     5941 cgctctggtg caacgtgcac gggcaccgcc atccgaagat cgacgaggcg gtgcgcgacc
     6001 agctcgaccg cgtggcccac tccacgatgc tcggcctctc gcatccgccg gcgatcgagc
     6061 tggcgcggcg cctcgtggag atcgcgccgc ccgggctgag ccgcgtcttc tactcggatt
     6121 cgggctcgac agcggccgag atcgctctga agatggcgtt ccagtactgg cagcagcagg
     6181 gcggagacgc agcgaaaaag acgaagttcg tctcgctccg cgaggcgtac cacggtgaca
     6241 cgatcggctc ggtctccgtc ggcggcatcg atctcttcca cgccacctac cggccgctcc
     6301 tcttcgacac gctgaaggcc gagaccggcg acgccgccga catggagcgg cttctcgtcg
     6361 agaacgaggg cgaagtcgcg gccgtgatcg tcgagccgct ggtgcagggc gcggccggca
     6421 tgctgctgca gccagatggc tacctgcgcc aggtccgcga gatgtgcgac cggcacggcg
     6481 cgctgctcgt gtgcgacgag gtcgcagtgg gcttcgggcg taccggccgg atgttcgcgt
     6541 gcgagcacga ggatgtcgcg cccgacttcc tctgtctcgc gaaggggatc accggcggct
     6601 acctgccgct cgccgccaca ctcgccacgg agcgtgtcta tgaaggcttc ctcggcgagc
     6661 accacgaatt ccgcaccttc ttccacggcc acacctacac cggcaatccc ctcgcctgcg
     6721 ccgcggctct cgcctcgctc gacgtgttcg aggaggagga cgtgatcggg cgcctccagg
     6781 ggaagatcgc gctgctcgag cgcctactcg aaccgatcgc ggcgctcccg tgcgtctcgg
     6841 aggtgcgcca gaagggcttc atggtgggca tcgagctcgg cggctttgcc gtcgaggagc
     6901 gcatgggcca ccaggtcacg ctcgcggcgc gcgagcgcgg tgcggtcatc cggccgctcg
     6961 gcgacgttgt ggtgctgatg ccgccgctcg ggatcgacga gagcgagcta atccgcctcg
     7021 tcgagatcac aggcgaggca atcgctgccg ccaccggcat cgcccgcgct gagcagcgcc
     7081 aggcggccta gtacagaatc cgccatacgc tcgcgggcat ggctgggcgt ggccgcgctg
     7141 ctcacgatca cgcccggccc cgaaatcgcg gtggtcgcgc gcagcgcgct cgagcgcggc
     7201 ttcggccccg ccgtgtggcg cgcgatcagc aggctcacgg gtgaaacacc tgagtttggt
     7261 tccgcatacg agaaccaaag tcaggagttg gatctcatcc tccggggcgt ccgggcgacc
     7321 ctcgtcccga agcgaaactg atattgggcc cctatatggg gctcaactca agtctcgtct
     7381 ctggagccct agcggctagc cctcgtaagg gacgaagtcc cgcttgcgcg cgccgcagac
     7441 ggggcagaac cagtcgtccg gaatgtcctg gaacagcgtt cccggcggga cgccgccgtc
     7501 ggggtcaccc tcggcggggt cgtagatgaa tccgcacgac tcgcagatcc acagctgttg
     7561 cgtggctgtc tccactgccc gcaggttagc gtccgtgcca ggtagggtgc gtccgtgcgc
     7621 ttcgaccgac cgccgcccgg agaggagctg aacccgggcg aagtgaccaa ggcgcgcgag
     7681 gcggcgtcgg tgatcgtcct ccgcgactcc gaggcgggtc ccgaggtgct gctcgtgcag
     7741 cgcaatccgg aggcgcgctt catgggcggc gcgtgggtgt tccccggcgg agcactcggc
     7801 aaggaggacg ccgggctcga gcagacggcg ctgcgagagc tcgacgagga ggccggtatc
     7861 gcgttgggcg acagcagcct cctcgtgccg ttctcgcgct ggatcacgcc ggcccaggtg
     7921 aaggttcgct tcgacacctt gttctacgtg acagaagcgc cagacggcgc cgaaccggtc
     7981 tgcgacggca aggagtgcgt cgaccttcgc tggatccggc cggccgacgc gctcgacgcc
     8041 gggcgccgcg gcgacctcat gctcgtgttc cccacgatca agcatctcga gcagctcgcc
     8101 gccttcgagt ccgtcgctca cgcgctcgag gtcgctcgct cgcgcaccgt cgagccggta
     8161 cagccgcgcg tggtggtgaa tgaaagctcc gcgcaggtgc tcttgcccgg tgaaccgggt
     8221 tacgacgatt gagcgctgcg ccgcaaccga gcggtcacgc ccgggttcat tcacttgcca
     8281 atgcgccgaa ccgtcttcac agccacggcg gccgttgccg catgcctgat ccttgccgcg
     8341 atcgcctttg cagacagctt cataggcagc tcgggcgacg acgtgctcgc aggcacgcct
     8401 ggtccggacc agatctacgg cgaggacggc aacgacacgc tgaacggcgg cgacgcgaac
     8461 gactacctcg aagccgggcc cggcgacgac caggtgaacg ccggcccagg cggggacctc
     8521 gtgatcggag gaaccggtag cgaccgcgtg gacgccggcc ccgggcccga cgtcgcctac
     8581 gccggaccgg gcagtgacga gctgctgggc ggagatggtc ccgacgtgct cttcggccag
     8641 atcggcgacg accgcatctt cgccggctcg ggcgacgatc gcctgtacac cggccgcggg
     8701 caggatttcg cggacggtgg cgcaggcgac gaccgcatcg ccgccgacct gggcgactcc
     8761 gtgctgaacg gcggcgcggg gcgagacttc atcgtcgccg tcgccggcgg cagcttcaca
     8821 gttgatggcg ggccgggcga cgaccgcatc acgctggggc ctggcaacga caaggtgcgt
     8881 gagggcggcg gagctgaccg gatctccagc ggcgcgggcg acgacacgat cgacgtgaaa
     8941 gacggccgcc gcgacagggt cgactgcggc gcgagccagg acacggtcgt cgccgacgac
     9001 aaggacaggc tcgtgcactg cgagctgcgc agcaccgccg ccaatcggta atgcttcccg
     9061 cgcgatggcg cggaacggac tgactgttgc tgtcacgggg ccgaccggcg acctcggcat
     9121 ggcgatcgtc tccgcgctcg agcgctctcg gcgcgtccag aagatcatcg gcatggcgcg
     9181 cagcccgttc gacccggcag cggcgggctg gcggaagacc gagtaccggc agggcgacgt
     9241 tcaggaccgt gcgagcgttc gccatgcggt gaagggggct gacgtggttg tgcacctcgc
     9301 cttcgccgtc gtcgaggcga gcgataccac gcgtgagatg aacgtcgagg gctcgcggat
     9361 cgtgttcgag gaggcttcgg cgggcggcgc cgaccgtatc tgctacgcgt cgagcgtcgc
     9421 ggcctacggc ttccacgagg acaacccgaa ctggctcacc gaggacatcc caccccgcgg
     9481 cacgcccgag cacttctact cacagcagaa ggccgaggtg gaggaggtgc tcggtcaggt
     9541 gctgctgcgc cgcccggcca cgacggcata cgtgttccgg ccatgcatcg tcgccggccc
     9601 gcgcgcccag acgatgctcg aggagatccc gtacctgcgg ctgtccgagc gcatgccgga
     9661 cgcggtcgtg cacctgctct cgttcatgcc gatcctcaag ccggtgatcc ccgaccctgg
     9721 cgtgcgcttc cagctagtgc acgaggacga cgtggcgtcg gcgttcctcg ccggcgtcct
     9781 cggaaagggc tcccccggtc cctacaacct cgcggcgaac ggcacgctca aggtgccgga
     9841 cctggccgac gcgctcggct ggtactcgat cccggtgccc gagttggccg tcgaagcgac
     9901 cgccgaggtg atcgcgcgcc ttccgatcgg cccggctcag ctgtcatgga tccagtcggc
     9961 gcgcaagccg gtgctgatga agacggaccg cgcccgcaag gagctcggct ggcgcccgca
    10021 gcacaccgcg cgggccacgc tcaaggagct cgtcgccgcc taccgcggcg acgccgagcg
    10081 cgtgcgctag gcggcgtagc gctcggcggc ccgcgcccgc cgcttcgcgg cctccacctc
    10141 gcggtccttc ggcggggcgt tggtcacgag gtcctcgagc aggtgcgcgg tcacgtgtgc
    10201 cacctcgtgc acggcgcggt tgaaggcctc ctcattggcc ttcgacggct tggtgaagcc
    10261 gctcaccttg cgcacgtact gcagcgcggc tgcctggacc tcctcactgg tcgcgggcgg
    10321 gtcgaagttg tggagcgttc ggatgtttct gcacatgcga cgagcctagc ctctcgctcg
    10381 aagttcgatc cggtccgcgg gcgcgagcag catcgtgatc ccgtcgcgcg gatcgcgcgg
    10441 ggacgcgcgt cgctcgcgca gcacgatcgc cgaccagggg cggcccgcgt cgagctccgg
    10501 tgcgagctcc gcggccagct cgcgcggcac ccagcccacc tgctctcccg agcccgagag
    10561 cacggcgatc gcgttcggat cgtgctcgtt gcccggatcg cgccgtagct cgagcggctg
    10621 cccgggcgcg gcgccgtccg actcgagcgc ctcgcggtga tgctggcctg cgcccgccac
    10681 gttcgacgtg cgcagaccgc gttcggcgag ctgcggggcg tcccgcgcga ggtactcgcc
    10741 ggactcggga tcgacgatcg aatagcccga gagccacacc tcgccgccgt cctccgggta
    10801 ccagtagcgc tcgtcgaaga cggcgtcgac gatcacagcg ccgcgcggta cagctccgcg
    10861 aagtcctcct ccgtgacctc gcggggattc acgagcgtac acccgtcgcc gacggccgcc
    10921 gccgcgagct ccggcacggc gtcctccggc acgcccgcct gcgacaagcg ggtgggcagc
    10981 ccgagcgagt cgacgagcga gcgcacgtgg ccggcgagag actcgccgac atctccgcgc
    11041 gaatccacgc cgagtacatc ggcgacgtcg cggtagcgct cggcgatgtc ctcgtcgccg
    11101 gcggcgttga agcggatcgc gtgcggcagg ttgatcgcgt tcgccatgcc gtgcggcacg
    11161 ccgtactggc cgccgcacgc gtgcgacatc gagtgcgcga tcccgaagaa cgcgcccgac
    11221 ccgcaggccg cgatcgcaag cgacgcggcc acgagcatgt tgccgcgcgc gtcttcgtcg
    11281 ccgggctcgg tgacggcacg ctcgaggttg tcgcggatga gccgcagcgc gtgtagcacc
    11341 agcgcgtcgc cgtgcgggct ccactccgtc gacacgtacg actcgatggc gtgcgtcatc
    11401 gcgtccatcc cgctcgcggc cgcgaccgcg ggtggcagcg tgcgcgtcgc ctcggggtcg
    11461 agcagagcga gccgcgggaa gagcggcacg tcgaccagct cgatcttcag ccgttcctcg
    11521 tggtccttca caaccgccgc ccacgagacc tcggaacccg tgccggccgt ggtggggatg
    11581 caggcgagcg gcgcgatcgg aagcgggctc cccatgccgt cctgttcgcg cggcagcgca
    11641 tagaggccct cgtagtcccg aaccgagccg ccgtgggcga acagcacatc ggccaccttg
    11701 gcggtgtcca tcacggagcc accgccgacc gctaggaacg agtccgcgcc cgcctcacgg
    11761 gcggcctccg cgcaacgcgc gaccgagtcg gtcgatgagt cctgcggcac gtcgtcgaac
    11821 acgcccgcga cctcgagccc gccgtcggtg agaccctcct ccacgcgctc gaccaggccg
    11881 gtaccgcgga tcacctggtc ggtcaccacc atcacgcgcg aggcgccctc cttcatgtac
    11941 tcgaagccga tgccctcgat cagcccgcgg ccggcgacga cccgggtggt cgcgttgtac
    12001 tggtagaagt ccttgaagcc cgtcatctga cgagctccga acgcctcgcc tgcctccatc
    12061 acgccgcctc gacgaggtct tcgcgccccg cgtcctcgag catcttgcgg tagcgcggga
    12121 acaccggctt cacgagcggc acgagcttga ggatcttcgc gaccgggccc ttcgccttca
    12181 tctgcccgcg cgccagagcg accgtcacgt tcaccttgcc gagccagaac ttgtgggccg
    12241 tgtcggcctc catcgtcatg atcacctcgg gctcgagctg gctctcgccg agttcgacct
    12301 ggccctcctc gccctcgacc atcttcaccg tgatctgcga ctcggggttg cggtaccggt
    12361 actggacggt ggtgttggcc ttctggaact tgggcgccag ctcctcgtcg gcgacgagct
    12421 cctggaacaa ccggccgatg tagtcgtaga cctcttgctc gtctctgaag tagggcatca
    12481 aggcttcctg tcttcgagtg cgggggcgac agattgttgt ggattgggga gatgtgacca
    12541 ccgcggaagg ccgccgcgct taccgttacc ctgttcaccg gcccgcaggc ggggatgtgt
    12601 gcggcgccgg aacctggggc gcctcagcgt gttccagcca ctgacacggg cccggcatgg
    12661 gcctgttttc cgcttcctcc cgacaccgga acagacactt gccagacagc ctccgacgcg
    12721 cgctcggcgc agccgtactc accgccgcgt tccttgcggt ccccgccggc gcgttcgccc
    12781 aggcgccggc tgaggcgccc accgaaaagg ttctctacaa ggacggctac gacgggcggt
    12841 acctgctcga cggcacttgg tggttccggc tggacccgca ggatcaggga ctctcgcaag
    12901 gcttccagcg ccagagctcg acgaccggtt ggacccaggt gaccgtgccg aacgcgtgga
    12961 acgcaactga catgtccccg gagagccagc gcggcacggt tggctggtac cggaaggact
    13021 tccgcgtacc gcggggggcc aagggcgcga cctggatgat ccgcttcgag tccgtgaact
    13081 accgcgcgac cgtgttcgtg aacggcaacc aggtgggtac gcacgagggc gcctatctgc
    13141 cgttcgaggt gccatccggc aagctgcgct ccggcgtgaa ccacctcgtg gtgcgggtcg
    13201 acagccgccg ttcgaacacc gaccttcccc cgctggtgga ccaggacaac ggcacgcccg
    13261 gcggcggctg gtggaactac ggcggtctgc tgcgcgaggt gtatctgcgc aaggtcaacc
    13321 aggtcgacat gaccgacttc ctcgcgcgtc cgctcctgcc gtgccgcaac tgcgatgcga
    13381 ccgttctcat ccgcgcgacg ctccacaacc tcggccgcaa gaagaaggta cgcgtgagcg
    13441 cgagggttgg cagccaggcc gcgcgcttcc ggcccgtaac cctgccggcg cgcggcacac
    13501 gccaggtgag cgcgaaggtg aagatcgcga acccgcgtct gtgggagccc ggcgaccctc
    13561 agctctacac ggtccgtggc gcggtgaacg tgggccgcaa ggtcggcgcg agctggtcgg
    13621 gcca
//
LOCUS       DASVXS010000015        16943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_3378, whole genome shotgun sequence.
ACCESSION   DASVXS010000015 DASVXS010000000
VERSION     DASVXS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16943
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF752_00775"
     CDS             <1..244
                     /locus_tag="VF752_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854106.1"
                     /translation="PKAHRVWVGTDIPGVEIPGQNKTERIVFRDAATGRELARSARVL
                     QMTQGSAVQPGYGGSVFFPVATGTLIKVTPRKAVAH"
     gene            complement(275..347)
                     /locus_tag="VF752_00780"
     tRNA            complement(275..347)
                     /locus_tag="VF752_00780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Arg,seq:ccg)
     gene            515..1861
                     /locus_tag="VF752_00785"
     CDS             515..1861
                     /locus_tag="VF752_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8854107.1"
                     /translation="MRRSALAIAALLPALAVGACGGGGSGGNSSSKAPPKYDGKPVTI
                     TLWHGFTSREAGILNGVVKDFERTHPTIKVKTVGGVTDDKIVAALRGGNPPDVAQSFS
                     TDNTGAFCGSGGWIDLKPYMDRDKVAVAQFPKAVQTYTTFDGKRCALPMMADVYGLYY
                     NKDMFKKAGIASPPKTFSELTADAKKLTVKNGDGSIKVAGFDPTFGFYESVHAHYAPL
                     FNAQWQDTSGKSTLATDPWMKLLRWQKDLVDWYGYGKLTKFNAGAGEEFSASNAFEKG
                     KLAMNMDGEFRTAFIADEAPHLNYGVAPMPVDDSQPELYGGGYVTGNIIGIPKTAKNK
                     AAAWELMKYLATDPHALATLTNGLKNVPTTATGLKSPEIKADGNFQPFLDIFENEHTQ
                     TNPPTAIGSANQELFQNFLAKWQAGKVPDSQLASGLKGVDQQIDAQIQNATAGQAP"
     gene            1869..2879
                     /locus_tag="VF752_00790"
     CDS             1869..2879
                     /locus_tag="VF752_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8854108.1"
                     /translation="MTVSGVAGARPGALAVAPERRAAKRKAQWRRRRMVLLMMSPWIV
                     GFTVFFGYPLIANAYLSFTHYDLLSSPRWIGTRNYDFMFSGDKQIWPAVKNTLWFIAI
                     AVPLQVIFAFGVAQIVARARSGVGFFRTVFYLPTLAPPVAATLAFVFLFNPATGPVNT
                     ILSKIGIEGPLWFQSPSWSKPSLTLLALWGVGNAMIIFLAAILDVPKHLHESAELDGA
                     GWFNRMRYVTLPSISPVILFSVVIGVIQGLQYFDQAYVASTVAAPGAAGNAADSSINL
                     GYPEGSTLMYPVLLYQQGFRYFNMGYAAAMSIMLLLVSLVVTLLIVRNSNRWVHAAGG
                     VK"
     gene            2880..3764
                     /locus_tag="VF752_00795"
     CDS             2880..3764
                     /locus_tag="VF752_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8854109.1"
                     /translation="MATAVPVSRPLVRREVPREIRRRRFLVAVAEHSVLIGLAIAFLA
                     PFVFIVLTSVMTDHQSLSTNLWPQPAKFSNYSQVFKDAPLLTYAGNTFLYATLATVGM
                     LISSVPVAYALACMRWRGRNVVFGLVLVSMMLPPQVTVVPQYVMWSKLHLIGTIWPLV
                     LPNWFGDAFSIFLLRQFFLTIPRDYADAARVDGLGEFRIMASVMTRLARPALVAVALF
                     SFLYAWNDFFGPLLYTGENQGHWTLSVGLAQFRHLHSVQWNLTMAATLLFMAPVIALF
                     FAAQKKFVEGVNLTGVKG"
     gene            3761..5008
                     /locus_tag="VF752_00800"
     CDS             3761..5008
                     /locus_tag="VF752_00800"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEX8854110.1"
                     /translation="MKVAVVGAGSTYTPELVSGLSRERERIDVHELALHDIDAERREV
                     VGGLAKRMLDRQRFDGELSVTGDLERALDGADAVLIQIRVGGQAARLADETIPAACGC
                     VGQETTGAGGFAKAMRTVPVVLEIAERARELAADGAWIVDFTNPVGIVTRALLDQGHR
                     AVGLCNVAINFQRRIAGLLGVEPARLRVDQVGLNHLTWVRAVWVDGENVLPDLLERHG
                     GVLADEVQLPLRLLEDLGAIPSYYLRYFYSEREVVAEQASGVPRARTVAEIEAELLAR
                     YRDESLAEKPALLERRGGAFYSEAATQLIASLRQGSGDVQVVDVRNGDTLAGLRPDDV
                     VEVPARIESGGPRPLEQRPLAPELLGLVQHVAAYERLTAEAAVTRDRATARLALLANP
                     LVREYELADELLESLLNPAGAVS"
     gene            5005..6048
                     /locus_tag="VF752_00805"
     CDS             5005..6048
                     /locus_tag="VF752_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX8854111.1"
                     /translation="MIALAVDGGGFKTDLALVRSDGELLSLVRGPMSSPHHVGLDGCI
                     EVIDGLLAEAVAGAGLDAGARPLTEVAHVLMAGADLPDEERELTEAVDASGWAAQTSA
                     ENDTFALLRAGSSNGWGVAVVCGAGFNCVGVSPDGRHARFLALGAITGDWGGGYDVGL
                     AAQMAAARAADGRGSSTSLESAVPAHFGLTDPFELAEEIHRGRVAARRLNELPPVVFA
                     ESEHDEVAAGIVDRLAGEVGTFAAAAIERLSLQDEQVPVVLGGGVMRGAPARLIDVVA
                     EQLSTVAPRARVVLELSPPIVGAALLGLDELGAGEEAQERVRREIEAAVARLGDGAAP
                     APAAVESGGPIDG"
     gene            6041..7129
                     /locus_tag="VF752_00810"
     CDS             6041..7129
                     /locus_tag="VF752_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854112.1"
                     /translation="MAEVRFEEATRLYQGTDSPAVDSLDLVLEDGELLVLVGPSGSGK
                     STALRMLAGLEEVDAGAIYIGDRDVTYVPPKERDVAMVFQNYALYPYLDVAANIGFPL
                     KMARVPKAERERRVSEAADMLGLTHLLSRKPGELSGGERQRTAMGRAIVRNPRVFLMD
                     EPLSNLDAKLRVQMRADIAALQQRLGVTTLYVTHDQVEAMTMGHRVAVLRDGKLQQCE
                     SPRVLYDHPANMFVAGFIGSPAMNLCHAELTPDGTAALDGWHVDIPRDAVSAAGARGL
                     RDVVLGLRPESLEFDAPEGIPARVEVVEELGADAYVFCRASLPGGDEKLVARTDCQCD
                     APQGQRVLLRPRPGQAHVFDPVTGERLD"
     gene            7129..8274
                     /locus_tag="VF752_00815"
     CDS             7129..8274
                     /locus_tag="VF752_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX8854113.1"
                     /translation="MPNGPTSATPPMLRDLNERTVLETIRAHAPISRAEISRRVGISK
                     PTVSQALRSLLDAGLVREAEANGGGLSYGATFFEPVAEAALVLGLDLGARFLRAAICD
                     LRGEVRARQDVDTGGRDALELLDTAVELKERLLAASGLEGDAVERVVLGVPGVIHTGS
                     MRVHLAENVPGLEGRAIGADLEERLGIRGSVENDVNLAAVGERWRGVARGVEDFVFLS
                     VGTGLGAGLVLGGELRAGRNGFAGELDFAHAGGGANDVDPSAPAIAEYAAKTGVRGDI
                     RSIFAAARSGDAPAQAVVDEEARRIALHITPIAAVADVELVVVGGGIGANGDLLLAPI
                     RERLADLLPFPPRVEVSSLGDAAVLTGALGVGLRGALDSVFANRGRR"
     gene            8293..8781
                     /locus_tag="VF752_00820"
     CDS             8293..8781
                     /locus_tag="VF752_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8854114.1"
                     /translation="MIVAIVSDTHLPRAGRTFPDRCVELMESAELILHAGDFIDPIVL
                     AEMEILGPPVKGVQGNIDLPELQRELPAELELELEGTRLAMLHDAGPSPGRLVRMRRR
                     FPNADALVFGHSHIPILEEHDGFQIFNPGSPTDKRRAPRHTMGLARVEDGRISFEHVD
                     LD"
     gene            8804..9772
                     /gene="rnz"
                     /locus_tag="VF752_00825"
     CDS             8804..9772
                     /gene="rnz"
                     /locus_tag="VF752_00825"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX8854115.1"
                     /translation="MDLDVFFLGTAGSAPTARRGLPATLIRRGGDRLLFDCGEGTQRQ
                     LLRSVGLLDLEEVFIGHFHADHFLGLPGLLKTYSLRGRDTPLTLHGPPGLRTLVEVLR
                     PVIGKTSFPVSINEVAPNEQLERDGYSVAAFEVAHRVRAYGYALIEDDRPGRFDEVRA
                     RELGVTPGPDFGRLQSGETVGDVRPEQVLGEPRRGRKIVLTGDTGPCEMTEIVAHGAD
                     LLVHEATFSIEEAERARETGHTTAHDAAMIAERAGVAMLALTHISQRYAGGEILDEAR
                     AVFENTIVPRDFDRVEIPFPERGGPVHHRANGERLPAQPVETEAGG"
     gene            9846..10274
                     /locus_tag="VF752_00830"
     CDS             9846..10274
                     /locus_tag="VF752_00830"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX8854116.1"
                     /translation="MAVGPLEYAVVEFEGNNFTGEIASAIGELVERNVVRVVDMVFVQ
                     KDEDGSVIALEVEDLDADVAAGYRSIGQDLVKVLNEDDVNIISDGLVPGSSVLLVVWE
                     NVWAARLSHAVRAANGRVLLQERVPEEVVQDALEAATARS"
     gene            10602..11189
                     /locus_tag="VF752_00835"
     CDS             10602..11189
                     /locus_tag="VF752_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX8854117.1"
                     /translation="MEAAEQQHHHEHHDHEAHARGSRNDVALSATLHCLTGCAIGEIA
                     GMAIGTAAALSNFGTVVLSIALAFVFGYSLTSMPLLRAGMALAAVIPIALASDTLSIG
                     TMEIVDNAIVVAVPGALDAGLGDLLFWGSLAFSLAVAGAAAFPVNRWLIDRGRGHAVV
                     HETGIHGGPDTRVVGVVTVFGFVFGAAVLIAEAVG"
     gene            complement(11192..14191)
                     /locus_tag="VF752_00840"
     CDS             complement(11192..14191)
                     /locus_tag="VF752_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8854118.1"
                     /translation="MRENWERFLSGDAVHGVRAPVADSWRRSLDAQVDPAASRPAPLA
                     ADADEASARWEVHPLAEAAALIRDRLASIANESQHLIVVSDADGMLLQLVGDTRVRSL
                     AADSMNFAEGALWSEAGAGTNAIGTALVADHAVQIFAAEHFMEVVQPWTCSAAPVHDP
                     ETGELLGVIDLTGLVKDVHPHSLALAISAARAVEAQLRYRRLERDDRLRARYHGQIIG
                     GSDRRALVGPTGGLLTDESRGWLRGADRLELPRGGGELVLPWGARAFAEPVGHEEAFI
                     VRKVGAQRPSRSRPQDDLHRLAEEQAALRRVAALVAAGAPPHEVFTAVTKELGQLLGV
                     SAMHMVRYSPDRTATGVASWSADGRDLPLGTRAHLDGTTAAGLVFMSGRPARKSGYDD
                     ASGEMAAVVRELGVRSSVGGPITVDGQLWGALIASSNKDEPLPRDTESRIAAFTELVA
                     TAISNTEARVDVARLAEEQAALRHVATLVAEAVPQKELFAAVAEEVGTLLRAEFATMV
                     CYESDRTANAVAAWASVVEHVEPSGRWTLDESDLAATILRTGRAARADEYDGAPGRIA
                     AYLRESFGVTCAVGSPIVVDGRVWGALFVHSTNARPLPPDTESRLESFTELVATAISN
                     AHARAEVRRLADEQAALRRVATLVARESPPAVVFGKVAEEVGLLVGADAAWMHCHMPD
                     GSARIVASWGSLDGFPVGTRLDLDGESVAAVVARTQRPARVDYAAASGALAASLHGLG
                     IRAGVGSPIVVDGHLWGGIVAATMRPEPMPADAESRIGEFTELVATAISNVQARADLA
                     ASRARIVAATDEERRRVVRDLHDGAQQRMVHTIVTLKLARRSLHRDRELTRTQLSEAL
                     EQEERAMVELRELAHGILPDVLTRGGLNAAVDSLASRTTVPVENGVSVDRLPSALEAT
                     AYFVVAEALTNVAKHAHARRAQVTVLVADDTLQVRVRDDGVGGARADGSGFQGLADRL
                     AVLEGSLRVESPANGGTLVEATIPVPR"
     gene            14341..14748
                     /locus_tag="VF752_00845"
     CDS             14341..14748
                     /locus_tag="VF752_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854119.1"
                     /translation="MSQRNVETVRRGYDAFNRGDLDDVRTIYAPDVTAHAGALWPAAG
                     DVSGVDGIIEAFASILATFENSEIVPEEFIELGDSVVVPTCWRGTLQSDSVIEQHLVA
                     VYTMRDGLVVRIGYFGDMDEALEDLREREGRTS"
     gene            14938..15483
                     /gene="pyrR"
                     /locus_tag="VF752_00850"
     CDS             14938..15483
                     /gene="pyrR"
                     /locus_tag="VF752_00850"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX8854120.1"
                     /translation="MTEKVVLDSEDFSRTLRRIAHEVVEKTGDERIAMVGIHRRGAVI
                     AKRLRELVEELADAPVPLGDLDISFYRDDLTLRSEQPEVHDTHIDFPIDGITVVLVDD
                     VLYTGRTVRAAIDSLFDYGRPQRVQLAVLADRGHRELPFRADYVGKNFPTARSERVNV
                     RIEEIDGTDEVTISEHAEVHT"
     gene            15483..16424
                     /locus_tag="VF752_00855"
     CDS             15483..16424
                     /locus_tag="VF752_00855"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX8854121.1"
                     /translation="MRNLLSIDDLTRDDIERILSRAHSFAQVSGREIKKVPTLRGRTV
                     INLFYESSTRTSSSFELAAKRLSADLVNIKSAGSSVDKGESLKDTVQTLSAYDPTAIV
                     IRSPHAGAANLVARWTAASVINAGDGKHEHPTQALLDLYTLRSRRRQIEGTKIWIVGD
                     VLHSRVARSNVLAFTRMGAEVTVCGPPTLIPRDFESLGCDVRYSLDGLEEADVVYALR
                     MQMERMTGSYVPSLREYAARFQIDSRRLAPHQLLMHPGPVNRGVELAAEVIDSPQALI
                     VQQVESGVVVRMAVLYELLAAGNAPSPVPAHTEPQPA"
     gene            16421..>16943
                     /locus_tag="VF752_00860"
     CDS             16421..>16943
                     /locus_tag="VF752_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854122.1"
                     /translation="MTPLDPRLDRPDTPPSDLLIKRAHLLDPRAGIDEPADLLVRRGE
                     VAEVGAPGSLEAPEGAEVVDGEGLHAFPAFVDPHVHLRTPGREDEEDIGSGTRAAAAG
                     GFCAILAMPNTDPVTDSASVLLSLRESAGSSSCSASTRPASRWYWSACRITPAERTGS
                     PSSVNAAAPASASS"
BASE COUNT         2550 a         5873 c         5956 g         2564 t
ORIGIN      
        1 cccgaaggct caccgcgtct gggtcggcac cgacattccc ggagtcgaga tccccggcca
       61 gaacaagacc gagaggatcg tcttccgcga cgcggccacg ggtcgagagc tcgcacgctc
      121 ggcgagagtc ctccagatga ctcagggaag cgcggttcag cccggctacg gcgggagcgt
      181 gttcttcccc gtcgccacgg gcacgttgat caaggtgacg ccgcgcaagg cggtcgctca
      241 ctagcgcact tgctgttcga acagcgatag aaagcgcgcc cggcaggatt cgaacctgcg
      301 acttctgcct ccggagggca gcgctctatc cactgagcta cgggcgcgag gctctgggag
      361 tctagccgcg gccggcatct ccatgggcac gtaacgggcc ttaacgcaat cagcccgtag
      421 agcccttgac tcggctgctt caagctgttt agagtcactt ctgggaaagg ttcctaacga
      481 gactaggttc tcggggtgag gagaggtgac tcggatgcgg cgtagcgctt tggcgattgc
      541 ggctctgctc ccggcgctcg cggtgggcgc gtgcggaggt ggcggaagtg gcggcaacag
      601 cagctcgaag gcgccgccga agtacgacgg caagcccgtc acgatcacgc tctggcacgg
      661 cttcacgagc cgcgaggccg gaatcctcaa cggcgtggtg aaggacttcg agaggacgca
      721 tccgactatc aaggtgaaga cggtcggcgg cgtgacggac gacaagatcg tggccgcact
      781 tcgcggcggc aatccgccgg acgtcgccca gtcgttctcg accgacaaca cgggcgcgtt
      841 ctgcggctcg ggcggctgga tcgacctgaa gccgtacatg gaccgcgaca aggtcgccgt
      901 ggcgcagttc cccaaggcgg tgcagaccta tacgaccttc gacggcaagc gctgcgcgct
      961 gccgatgatg gccgacgtct acggcctcta ttacaacaag gacatgttca agaaggcggg
     1021 gatcgcctcc ccgccgaaga cgttctccga gctgaccgcc gacgccaaga agctcaccgt
     1081 caagaacggt gacggctcga tcaaggtcgc cggcttcgat cccacatttg gcttctacga
     1141 gagcgttcac gcgcactacg cgccgctctt caacgcgcag tggcaggaca cctccggaaa
     1201 gtccacgctc gccaccgacc cgtggatgaa gctgctgcgc tggcagaagg acctcgtgga
     1261 ctggtacgga tacggcaagc tcacgaagtt caacgcgggc gcgggcgagg agttctcggc
     1321 ctcgaacgcg ttcgagaagg gcaagctcgc gatgaacatg gacggcgagt tccgcaccgc
     1381 cttcatcgcc gacgaggcgc cgcacctcaa ctacggcgtc gcgcccatgc ccgtcgacga
     1441 ctcgcagccc gagctatacg gcggcggcta cgtgacgggg aacatcatcg gcatcccgaa
     1501 gaccgccaag aacaaggcgg ccgcgtggga gctcatgaag tacctcgcga ccgacccgca
     1561 cgcgctcgcg acgctcacga atgggctgaa gaacgtgccg accacagcta ccgggctgaa
     1621 gtcgcccgag atcaaggcgg acgggaactt ccagccgttc ctcgacatct tcgaaaacga
     1681 gcacacgcag acgaacccgc ccacggcgat cgggagcgcg aaccaggagc tgttccagaa
     1741 cttcctcgcc aagtggcagg cgggcaaggt gccggattcg cagctggcgt cggggctcaa
     1801 gggcgtggac cagcagatcg acgctcagat ccagaacgcc accgccgggc aggctccgtg
     1861 agcgcgcgat gaccgtctcg ggcgtggcag gcgcccggcc gggcgcgctc gcggtcgctc
     1921 ccgagcgacg cgcggcgaag cgcaaggctc agtggcggcg gcgccggatg gtgctgctga
     1981 tgatgagccc gtggatcgtc ggcttcaccg tgttcttcgg ctatccgctg atcgcgaacg
     2041 cgtacctgtc gttcacgcac tacgacctgc tcagctcgcc gcgctggatc ggcacgcgga
     2101 actacgactt catgttcagt ggcgacaagc agatctggcc cgcggtgaag aacacgctct
     2161 ggttcatcgc gatcgccgtc ccgctgcagg tcatcttcgc gttcggcgtc gcgcagatag
     2221 tcgcccgcgc gcgcagcggg gtgggcttct tccgcacggt gttctacctg cccacgctgg
     2281 cgccgccggt cgcggccacg ctcgcgttcg tgttcctgtt caacccggcc accgggcccg
     2341 tgaacacgat cctcagcaag atcggcatcg aggggccgct gtggttccag tcgccaagct
     2401 ggtcgaagcc gtcgctcacg ctgctcgcgc tgtggggcgt gggcaacgcg atgatcatct
     2461 tcctcgcggc gatcctcgac gtgccgaagc acctgcacga gtcggccgag ctcgacggcg
     2521 ccggctggtt caaccggatg cgctacgtga cgctgccgag catcagcccg gtgatcctgt
     2581 tctcggtcgt catcggggtc atccaggggc tccagtactt cgaccaggcg tacgtggcga
     2641 gcacggtcgc cgccccgggc gcagcgggca acgcggccga cagctcgatc aacctcggct
     2701 accccgaggg ctcgacgctg atgtaccccg tgctgctcta ccagcagggc ttccgctact
     2761 tcaacatggg ctacgcggcc gcgatgtcga tcatgctgct gctggtctcg ctcgtggtca
     2821 cgctgctgat cgtgcgcaac tcgaaccgct gggtgcacgc ggcgggaggg gtgaagtagg
     2881 tggcgaccgc cgtcccggtc agccgcccgc tcgtccgccg tgaggtgccc cgcgagatac
     2941 gtcgccggcg cttcctggtc gcggtcgcgg agcactcggt gctgatcggg ctcgcgatcg
     3001 cgttcctggc gccgttcgtg ttcatcgtgc tcacgtcggt gatgacggac catcagtcgc
     3061 tgtccacgaa cctgtggccg cagccggcga agttctcgaa ctactcgcag gtcttcaagg
     3121 acgcgccgct gctcacctac gcgggcaaca cgttcctgta cgcgacgctc gcgaccgtcg
     3181 gcatgctgat ctcgagcgtg cccgtcgcgt acgcgctcgc gtgcatgcgc tggcgcggcc
     3241 ggaacgtcgt attcgggctc gtgctcgtgt cgatgatgct tccgccgcag gtgacggtgg
     3301 tgccgcagta cgtgatgtgg tcgaagctgc acctgatcgg cacgatctgg ccgctcgtct
     3361 tgccgaactg gttcggcgac gcgttctcga tcttcctgct gcggcagttc ttcctcacga
     3421 tcccgcgcga ctacgcggac gccgcgcgcg tggacggcct cggcgagttc cggatcatgg
     3481 cgtcggtgat gacgcgcctc gcgcggccgg cgctcgtggc ggtggcgctt ttcagcttcc
     3541 tctacgcctg gaacgacttc ttcgggccgc tgctctacac cggtgagaac cagggccact
     3601 ggacgctgtc ggtcggcctc gcccagttcc gccatctgca ctcggtgcag tggaacctga
     3661 cgatggccgc gacgctcttg ttcatggcgc cagtgatcgc gctcttcttc gccgctcaga
     3721 agaagttcgt cgagggcgtg aacctgacgg gggtgaaggg atgaaggtcg cggtcgtggg
     3781 cgcgggctcg acctacacgc ccgagctcgt gtcgggtctc tcgcgcgagc gcgagcggat
     3841 agacgtgcac gagctcgcgc tgcacgacat cgacgccgag cgccgcgaag tggtgggcgg
     3901 gctcgcgaag cggatgctcg accggcagcg attcgacggc gagctgtccg tcaccggcga
     3961 cctcgaacgc gcgctcgacg gcgccgatgc ggtgctgatc cagattcgcg tgggcggcca
     4021 ggcggcgcgg ctcgccgacg agacgatccc ggccgcctgc ggctgcgtgg gccaggagac
     4081 gaccggcgcc ggcggcttcg ccaaggccat gcgcacggtg ccggtggtgc tcgagatcgc
     4141 ggagcgcgcg cgcgagctcg cggccgacgg cgcctggatc gtcgacttca ccaaccccgt
     4201 ggggatcgtc acgcgcgcgc tgctcgacca gggccaccga gccgtcggcc tctgcaacgt
     4261 cgccatcaac ttccagcgcc ggatcgccgg cctgctgggg gtcgagcccg cacggctaag
     4321 ggtggaccag gtcggcctca atcacctcac ctgggtccgc gccgtgtggg tcgacggcga
     4381 gaacgtgctg cccgacttgc tcgagcgcca cgggggcgtg ctggccgacg aggtccagct
     4441 gccgctgcga ctactcgagg acctcggcgc gatcccgtcc tactacctgc gctacttcta
     4501 ctcggagcgc gaggtggtgg cggagcaggc gtcgggcgtg ccgcgcgctc gcacggtggc
     4561 cgagatcgag gccgagctac tcgcgaggta ccgcgacgag tcgctcgccg agaagcccgc
     4621 gctgctcgag cgccgcggcg gcgccttcta cagcgaggcg gccacccagt tgatcgcgtc
     4681 gcttcgacag ggcagcggcg acgtgcaggt ggtggacgtg cgcaacggcg acacgctcgc
     4741 gggtctccgc cccgacgacg tggtcgaggt gccggcgcgc atcgagtccg gcgggccgag
     4801 gccgctcgag cagcgcccgc tcgcgccgga gctgctcggg ctcgtgcagc acgtggccgc
     4861 ctacgagcgg ctgacggcgg aggccgcggt cacgcgcgac cgcgcaaccg cgcggcttgc
     4921 gctgctggcg aatccgctcg tgcgcgagta cgagttggcc gacgaactgc tcgagagcct
     4981 gctgaacccg gcgggggctg tctcgtgatc gcgctcgcgg tcgacggcgg cggcttcaag
     5041 acggatctcg cgctcgtgcg ctcggacggc gagctgctgt cgctcgtgcg cgggccgatg
     5101 agctcgccgc accatgtggg cctcgacggc tgcatcgagg tgatcgacgg cctgctcgcc
     5161 gaagcggtgg cgggcgctgg gctcgacgcg ggcgcgcgcc ccctcaccga ggtagcccac
     5221 gtgctgatgg caggcgccga cctgcccgac gaggagcgcg agctgaccga ggccgtggac
     5281 gcgagcggtt gggcagcgca aacctccgcc gagaacgaca cgttcgcgct cctgcgcgcg
     5341 ggctcctcga acggatgggg cgtcgcggtc gtttgcggtg cgggattcaa ctgcgtgggc
     5401 gtctcgcccg atggccgcca cgcgcgcttc ctcgcgctcg gcgcgatcac cggcgactgg
     5461 ggcgggggct acgacgtcgg gctcgcggcg cagatggcgg ctgcgcgggc ggctgatggc
     5521 cgcggctcga gtaccagcct ggagtccgcc gtgcctgcgc atttcgggtt gaccgatccc
     5581 ttcgagctcg ccgaggagat tcaccgcgga cgcgtggccg cgaggcgcct gaacgagttg
     5641 ccgccggtcg tgttcgcgga gtccgagcac gacgaagtgg cggcgggcat agtcgaccgc
     5701 ctcgccggcg aggtcggcac gttcgccgcg gcggcgatcg agcgcctgtc gctccaggac
     5761 gagcaagtgc cggtcgtgct tggcggcggc gtcatgcggg gtgcgcccgc gcgcctcatc
     5821 gacgtcgtcg ccgagcagct cagcacggtc gcgccgcgtg cccgggtggt cctggaactg
     5881 tcgccgccga tcgttggagc ggcgctgctc gggctcgacg agctcggcgc gggggaggag
     5941 gcgcaggagc gcgtgcggag agagatcgaa gcagccgtcg cccgacttgg cgacggcgcc
     6001 gcgccggcgc ccgctgctgt ggagtcagga gggccgatcg atggctgaag tgcgcttcga
     6061 ggaggcgacg aggctgtatc agggaaccga ctcgccggcg gtcgactcgc tcgacctagt
     6121 gctcgaggac ggcgagctgc tcgtgctcgt gggcccgtcg ggatccggca agtccaccgc
     6181 gctgcgcatg cttgcggggc tcgaagaggt cgacgcgggt gcgatctaca tcggggaccg
     6241 cgatgtgacg tacgtgccac cgaaggaacg cgacgtggcc atggtgttcc agaactacgc
     6301 gctctacccg tacctggacg tggcggcgaa catcggcttc cccctgaaga tggcccgtgt
     6361 gccgaaggcc gagcgcgagc gccgcgtgtc ggaagcggcg gacatgctcg gcctcacgca
     6421 cctgctttcg cgcaagcccg gcgagctgtc gggcggcgag cgccagcgca ctgcgatggg
     6481 ccgcgcgatc gtgcgcaacc cgcgcgtgtt cctcatggac gagccactct cgaacctcga
     6541 cgcgaagctg cgcgtgcaga tgcgcgccga catcgcggcg ctgcagcagc ggctcggggt
     6601 cacgacgctg tacgtgaccc acgaccaggt ggaggcgatg acgatgggcc accgcgtggc
     6661 cgtgctgcgc gacgggaagc tgcagcaatg cgaatcgccg cgcgtgctct acgaccaccc
     6721 ggcgaacatg ttcgtggcgg gcttcatcgg gtcgccggcg atgaacctct gccacgccga
     6781 gctcacgccc gacggcaccg ctgcactcga cggctggcac gtcgacatcc cgcgcgatgc
     6841 cgtgtcggcg gcgggtgcgc gcggattgcg tgacgtcgtc ctcggcctgc ggcccgagtc
     6901 gctcgagttc gatgcgcccg aggggatccc ggcgcgcgtc gaggtcgtcg aggagctcgg
     6961 cgccgacgcc tatgtctttt gcagggcctc gctgcctggc ggcgacgaga aactcgtcgc
     7021 gcgaaccgac tgccagtgcg acgcgccgca gggccagcgg gtgctgcttc ggccgcgtcc
     7081 cggccaggcg cacgtgttcg accccgtcac cggcgagcgg ctcgactgat gcccaacggc
     7141 cccacctcag ccaccccgcc gatgctgcgg gacctgaatg agcggaccgt gctcgagacg
     7201 atccgcgcgc acgctccaat ctcacgcgcg gagatctcgc ggcgggtggg catctcgaag
     7261 cccaccgtct cccaggcgct gcgatcgctg ctcgacgccg gcctcgtgcg cgaggccgag
     7321 gcgaacgggg gagggctcag ctacggtgcc acgttcttcg agccggtcgc cgaggctgcg
     7381 ctcgtgctcg gcctcgacct cggcgcgcgc ttcctgcgtg ctgcgatctg cgacctgcgc
     7441 ggcgaggtcc gggcgcgaca ggacgtggac acgggcgggc gcgatgcgct cgagctgctc
     7501 gacacggcgg ttgagctcaa ggagaggctg ctcgccgcat ccggcctgga gggtgacgcg
     7561 gtggagcgcg tggtgctcgg cgtgccgggc gtgatccaca ccggctcgat gcgtgtgcac
     7621 ctcgccgaga acgtgccggg gctcgagggc cgcgcgatcg gagccgacct cgaggagcgc
     7681 ctcggcatcc gcgggagcgt ggagaacgac gtgaacctcg cggcggtggg ggagcgctgg
     7741 cgcggcgtgg cgcgcggcgt cgaggacttc gtgttcctct cggtcggcac cggccttggc
     7801 gcagggctcg tcctcggggg cgagctgcgc gccgggcgaa acggcttcgc cggcgagctc
     7861 gacttcgcgc acgcgggcgg cggcgcgaac gacgtcgatc cctcggcccc ggcaatcgcc
     7921 gagtacgccg cgaaaaccgg cgtgcgcggg gacatccgct cgatcttcgc cgccgcccgc
     7981 tcgggcgatg cgccggcaca ggcggtggtg gacgaggagg cgcggcggat cgccctgcac
     8041 atcactccga tcgccgcggt cgccgacgtg gagctcgtcg tggtcggcgg tggtatcggc
     8101 gcgaacggcg acctgctgct tgcgccgatc cgcgagcgcc tcgccgacct gctgcccttc
     8161 ccgccgcgtg tggaggtgtc gagtctcggc gacgccgcgg tgctcacggg cgctctcggc
     8221 gtgggccttc ggggcgctct cgacagcgtg ttcgccaacc gcggccgccg ctgagcacta
     8281 cgctcccgcg gcgtgatcgt cgcgattgtc tccgacaccc acctgccacg tgccggccgc
     8341 acgttccccg accgctgcgt cgagctcatg gagtccgccg aactgatcct gcacgccggc
     8401 gacttcatcg atcccatcgt gctcgccgag atggagatcc tcggcccgcc ggtgaaggga
     8461 gtgcaaggca acatcgatct gcccgagctg cagcgcgagt tgccggcgga gctcgaactc
     8521 gagctcgagg gcacgcggct cgcgatgctg cacgacgccg gtccgtcgcc ggggcggctc
     8581 gtgcgcatgc gccggcggtt cccaaatgcc gacgccctcg tgttcgggca ctcgcacata
     8641 cccattctcg aagagcacga cggcttccag atcttcaatc ccggaagccc caccgacaag
     8701 cgccgggcgc cgcgtcacac gatgggtctc gcgcgcgtgg aggacggcag gatctccttc
     8761 gagcacgtcg acctcgactg agcggcgcga agataggcct ctgatggacc tcgacgtgtt
     8821 ctttctcggc accgccggct ccgccccaac ggcgcggcgc ggcctgcccg ccacgctgat
     8881 ccggcgcggc ggcgaccgcc tgctcttcga ctgcggcgag ggcacgcagc gccagctgct
     8941 gcgctcggtc ggattgctcg atctcgagga ggtgttcatc ggccacttcc acgccgacca
     9001 cttcctcgga ctgccggggc tgctgaagac ctacagcctg cgcgggcgcg acacaccgct
     9061 cacgctccac ggaccgccgg ggcttcgcac gctcgtcgag gtgctgcggc ccgtgatcgg
     9121 caagacgagc ttcccggtga gcatcaacga ggtcgcgccg aacgagcagc tcgagcgcga
     9181 cggatacagc gtcgcggcct tcgaggtggc acaccgagtt cgcgcgtacg gctacgcgct
     9241 gatcgaggac gaccgcccgg gccgcttcga cgaagtgcgc gcgcgcgagc tcggcgtcac
     9301 gcccgggccc gacttcggcc gcctgcagtc gggcgagacg gtcggcgacg tgcgcccaga
     9361 gcaggtgcta ggcgagccgc ggcgcgggcg caagatcgtg ctaacgggcg acaccgggcc
     9421 gtgcgagatg accgagatcg tggcccacgg cgcggacctg ctcgtgcacg aggccacgtt
     9481 cagcatcgag gaggcggaac gcgcgcgcga aacgggccac acgacggcac acgacgcagc
     9541 catgatcgcc gagcgcgccg gtgtggcgat gctcgcgctc actcacatct cacagcgcta
     9601 cgcgggcggt gaaatcctcg acgaggcgcg cgccgttttc gagaacacga tcgtgccgcg
     9661 cgacttcgac cgcgtggaga tcccgttccc ggagcgcggc ggccccgtgc accaccgtgc
     9721 gaacggcgag cgcctgccgg cgcagcccgt cgagaccgaa gccggcggct gaatcacccc
     9781 tttcgggcga tgccgcggcg catgccctcc gtgatcgtgg ggggcgattc ggaaaggagg
     9841 tccgcgtggc cgtgggaccg ctggaatatg ccgtcgtcga gttcgagggc aacaacttca
     9901 ccggtgagat cgcctcggcg atcggcgagc tagtcgagcg caacgtggtg cgcgtcgtgg
     9961 acatggtctt cgtccagaag gacgaggacg ggtcagtcat cgcgctcgag gtagaggacc
    10021 tcgacgcgga cgtggccgcc ggctaccgga gcatcgggca ggacctggtg aaggtcctca
    10081 acgaggacga cgtgaacatc atcagcgacg ggctcgtgcc gggctcgtcg gtgctgctcg
    10141 tggtctggga gaacgtctgg gccgcgcggc tgtcgcacgc tgtgcgcgcc gctaacggcc
    10201 gggtgctgct ccaggagcgc gtcccggaag aagtggtgca ggacgcactc gaggccgcaa
    10261 cggcgcgctc gtagcgagaa aggagacggg acatgcttag aggaggacgc ggcggcccgg
    10321 gcttggtccg gggtgtggcg cgcacagccg tagtcgccgg cactgcgacc ggcgtttcga
    10381 accgggtgag ccgtcggcag gcggcccgct gggaacagca ggatgaggaa gcggccgcgg
    10441 ctcaggctcc accggaagcg gcagcggcac ccgcgacaag ctcgaccgac gagctcgaaa
    10501 ggctcgcgca gttgcattcg agcggagccc ttacggacga agagttcgcg tccgccaagg
    10561 cgcagctgct cggctaacta agggccctag gcttcctggt tctggaagcc gccgaacaac
    10621 agcatcatca cgagcaccac gaccatgagg cccacgcccg cggcagccgt aacgatgtcg
    10681 cgctaagcgc aacgctccac tgcctgaccg gctgtgcgat cggcgagatc gccggcatgg
    10741 cgatcggtac cgcggcggct ctgagcaact tcgggaccgt tgtcctctcg atcgccctcg
    10801 cgttcgtctt cggctacagc ctcacgagca tgccgctgct acgggcgggc atggccctcg
    10861 ccgccgtgat cccgatcgcg ctcgcgtcgg acacgctcag catcgggacg atggagatcg
    10921 tcgacaacgc gatcgtcgtg gccgtgccgg gcgcgcttga cgcagggctc ggcgatctgc
    10981 tcttctgggg cagtctcgcg ttctcgctgg cggtcgcggg tgcggcggcg ttccccgtga
    11041 accgctggct gatcgaccgc ggccgcggcc acgcggtcgt gcacgagacg ggcatccatg
    11101 gtggcccgga cacccgcgtg gtgggcgtcg tcactgtctt cggcttcgtg ttcggcgcgg
    11161 cggtgctgat cgccgaggcg gtgggatagc gctagcgggg caccgggatg gtggcctcga
    11221 ccagtgtgcc gccgttcgcg gggctctcca cccggaggct gccctcgagc acggccagcc
    11281 ggtcggcgag cccttgaaag ccgctcccgt cggcccgggc gccgccgaca ccgtcgtcgc
    11341 gcacgcgtac ctgaagcgtg tcgtccgcga cgagcaccgt gacctgcgct cgcctcgcat
    11401 gggcgtgctt cgcgacgttg gtcagggctt cggcaacgac gaagtaggcc gtggcctcca
    11461 gcgcggaggg caggcggtcc acggacacac cgttctcgac tggaaccgtc gtccgcgaag
    11521 ccagtgagtc cacagcggcg ttcagccctc cgcgggtgag tacgtccggc aggatcccgt
    11581 gcgctagctc ccgcaactcc accatcgcgc gttcttcctg ctccagggcc tcggacagct
    11641 gcgtgcgggt cagctctcgg tcgcggtgca gcgaccggcg cgcgagcttc agcgtgacga
    11701 tggtgtggac catgcgctgc tgagccccgt cgtgcaggtc gcgaaccaca cggcggcgct
    11761 cctcgtccgt cgccgcgacg atccgtgcgc gcgaggcggc caggtccgcg cgcgcctgga
    11821 cgttcgagat cgccgtcgca accagctctg tgaactcgcc gatgcgcgac tccgcgtccg
    11881 ccggcatcgg ctccggccgc atcgtcgccg cgacgatccc gccccaaaga tgcccgtcga
    11941 cgacgatcgg gctgcccacg ccggcacgta tgcccaaccc gtgcagcgac gcggcgagcg
    12001 ccccactggc ggcggcgtag tcgactcgag ccgggcgctg cgtccgagcc acgaccgcgg
    12061 cgacgctctc cccatcgagg tcgaggcggg tgcccaccgg gaagccgtcg aggctgcccc
    12121 aggaagcgac gattctggcg ctgccgtcgg gcatgtggca gtgcatccac gcggcatcgg
    12181 cgccgacgag cagcccgacc tcctcagcta ccttgccgaa gaccacagcc ggcggcgact
    12241 cccgcgccac cagggtcgcc acgcgccgga gcgccgcctg ctcgtcggca agacggcgca
    12301 cctcggcgcg agcgtgggcg ttcgaaatgg cggtggccac gagctcggtg aagctctcca
    12361 gacgcgactc ggtgtccggg gggagcggcc gggcgttcgt cgagtgcacg aacagcgcgc
    12421 cccacacacg cccgtctacg acgatcgggc tcccaacggc gcaggtgacc ccgaagctct
    12481 cgcgcagata ggccgcgatg cgcccgggcg caccgtcgta ttcgtctgcc cgcgcggctc
    12541 gtccggtccg caagatcgtg gccgccagat cgctttcgtc gagcgtccac cgaccgctgg
    12601 gctccacgtg ctcgacaaca cttgcccacg ccgccacggc gttcgccgtg cggtcactct
    12661 cgtaacagac catcgttgcg aactccgccc tcaggagcgt gccgacctct tcggcgactg
    12721 cagcgaagag ttctttctgg ggcacggcct ctgcgaccag cgtcgccacg tggcgcagcg
    12781 cggcctgctc ctccgccagc cgtgccacgt cgactcgcgc ttcggtgttg gagatcgccg
    12841 ttgcgacaag ttccgtgaac gccgcgatcc tcgactcggt gtcgcgcgga agcggctcgt
    12901 ctttgttcga cgacgcgatc agcgcgcccc acagctgccc gtcgaccgtg atcggccctc
    12961 cgaccgacga gcgaactccc agctctcgga ccaccgcggc catctcgccg gaagcgtcgt
    13021 cgtagccgct ctttcgcgcc gggcgcccgc tcatgaacac cagtccggcg gcggttgtgc
    13081 catcaaggtg ggcgcgtgta ccgagcggca ggtcgcgccc gtcggcgctc cagctcgcga
    13141 caccggtcgc ggtgcgatca ggcgaataac ggaccatgtg catcgcgctc acgccgagca
    13201 gctggcccag ctctttcgtg acggccgtga atacctcgtg cggcggcgcg ccggctgcga
    13261 ccaacgccgc cacgcgccgc agcgctgcct gttcctccgc gagcctgtga aggtcgtctt
    13321 ggggacgact gcgtgacggg cgctgggcgc caaccttgcg cacgatgaag gcctcctcgt
    13381 gccccaccgg ctccgcaaac gcgcgcgctc cccagggaag cacgagctcg ccgcctcccc
    13441 gagggagctc gagacgatcg gcgccgcgca gccatccgcg cgattcgtcg gtgagtagcc
    13501 cgccggtcgg gccgaccagc gcgcgacgat cggagccgcc gatgatctgg ccgtggtagc
    13561 gagcgcgtag ccggtcgtcg cgctccagcc gccgatagcg aagttgcgct tcgaccgccc
    13621 gcgcggcgct gatcgcgaga gccaggctgt gcgggtggac gtctttcacg agcccggtga
    13681 ggtcgatgac gccgagcagc tcgcccgtct ccgggtcgtg tacgggggcg gccgagcagg
    13741 tccacggctg gacgacttcc atgaagtgct cggcggcgaa gatctgcact gcgtgatccg
    13801 cgaccagcgc cgtcccgatc gcgttggttc cggcgcccgc ctcgctccac agtgcaccct
    13861 cggcgaagtt catcgagtcg gcggctagcg agcggactct ggtgtcgcca acgagctgga
    13921 gcagcatccc gtcggcgtcg ctcacgacta tcaggtgctg cgactcgttg gcgatgctcg
    13981 cgaggcggtc acggatcagc gccgccgctt ccgccagcgg atgcacctcc caccgagctg
    14041 acgcctcgtc agcgtcggcc gcaagcggag ccggccggct cgcggccggg tcgacctgag
    14101 cgtctagcga ccgccgccac gagtccgcga ccggcgcgcg tacgccgtgg acggcgtcgc
    14161 cgctgagaaa ccgctcccag ttctcccgca cttcgcgggc ccgcgaagcg ggcggcgtcc
    14221 tcgcatcgat ggccagccat ggatgaggga tggacggatt gtcccacccg gctgaaccgg
    14281 gcgcacgggg cggatcgtgt acctggtcag cgcctcgcgt ggcgcagata atgggcgcag
    14341 atgtcgcagc gcaacgtgga gaccgtgcgc cgcgggtacg acgctttcaa tcgaggcgac
    14401 ctcgacgacg tacgcacgat ctacgcaccg gacgtgaccg cccacgccgg agcgctgtgg
    14461 cccgcggcgg gcgatgtctc cggcgtggac gggatcatcg aggcgttcgc atcgatcctc
    14521 gcgacgttcg agaacagcga aatcgtcccg gaggagttca tcgaactcgg cgattcggtc
    14581 gtggtgccga cgtgctggcg cggcaccctg caaagcgaca gcgtcatcga gcagcacctg
    14641 gtggccgtgt acacgatgcg cgacggcttg gtcgtccgca tcggatactt cggggacatg
    14701 gacgaagctc tcgaggatct gcgggagcgg gagggccgga cgtcctagcg ctgccgctcg
    14761 gttgccaggg gaactgggcc tcccttttcg atcgttgaat cgatcaacgg tcaaaaaggc
    14821 aataagggga gcggtgcgcg aaagcgcggc cagccgtgcg cacggcgtcg gatcgcgttg
    14881 ctacagtcgc gcctgcaagt cctttaacca ggtccggtga ggccaggaag gatgggaatg
    14941 accgagaagg tggtcctcga cagcgaggac ttcagccgta cgctgcgccg catcgcgcac
    15001 gaggtggtcg agaagaccgg cgacgagcgt atcgcgatgg tcgggatcca tcgccgaggc
    15061 gcggtcatcg cgaagcggct ccgggagctc gtcgaggagc tcgccgacgc gccggtcccg
    15121 ctcggcgatc tcgacatctc cttctaccgc gacgacctca cgctgcgctc cgagcagccc
    15181 gaggtgcacg acacccacat cgatttcccg atcgacggga tcaccgtggt cctcgtcgac
    15241 gacgttctct acaccggccg cacggtgcgc gccgcgatcg actcgctctt tgactacggg
    15301 cgcccgcagc gggtgcagct cgcggtgctg gcggaccgcg gccaccgcga actgcccttc
    15361 cgcgcggact acgtggggaa gaacttcccg acggccagaa gcgagcgcgt gaacgttcgt
    15421 atcgaggaga tcgacggaac cgacgaggtc acgatctccg agcatgcgga ggtacacacc
    15481 tgatgcggaa cctgctgtcg atcgacgacc tcacgcgcga cgacatcgaa cgcatcctca
    15541 gccgcgcgca cagcttcgcc caggtgtccg gccgtgagat caagaaggtg ccgacccttc
    15601 gcggccggac cgtgatcaac ctcttctatg agtcctccac gcgcacgagc tccagcttcg
    15661 agctcgccgc caagcgactc agcgctgacc tcgtgaacat caagtccgcg ggctcgtcgg
    15721 tggacaaggg cgagtccctc aaggacacgg tccagacgct ctcggcctac gacccgaccg
    15781 cgatcgtgat ccgctcgccg cacgccggcg ccgccaacct ggttgcgcgc tggaccgccg
    15841 cctcggtgat caacgcgggg gacggcaagc acgagcaccc gacgcaggcg ctgctggacc
    15901 tttacacgct acgaagccgg cgccggcaga tcgagggcac gaagatctgg atcgtcggcg
    15961 acgtgcttca cagccgcgtg gcgcgctcga acgtgctcgc cttcacgcgc atgggcgccg
    16021 aggtgaccgt gtgcgggccg ccgacgctga tcccgcgcga cttcgagtcg ctcggctgcg
    16081 acgtgcgcta ctcgctcgac ggcctcgagg aggccgacgt ggtgtacgcg ctgcgcatgc
    16141 agatggagcg catgacgggc tcctacgtgc cctcgctgcg ggagtacgcg gcgcgcttcc
    16201 agatcgacag ccgccggcta gccccgcacc agctcctgat gcaccccggc ccggtgaacc
    16261 gcggcgtgga gctggccgcg gaggtgatcg actcgccgca ggcgctgatc gtgcagcagg
    16321 tcgagtccgg agtggtggtg aggatggcgg tcctctacga gctgctcgcc gccggcaacg
    16381 ctccctcacc ggtcccggcg cacacggagc cgcagcccgc atgacgccgc tcgacccgcg
    16441 actcgatcgc ccggacaccc cgccgagcga tctgctcatc aagcgcgcac acctgctcga
    16501 cccgcgcgcc ggcatcgacg agcccgccga cctgctcgtg cgccgcggcg aggtggcgga
    16561 ggtcggggcg cccggctcgc tggaggcgcc tgagggcgcc gaggttgtcg acggcgaggg
    16621 gctccacgca ttccccgcgt tcgtggaccc gcacgtacac ctgcgcacgc ccggccgcga
    16681 ggacgaggag gacatcggct cgggcacgcg tgccgcagcg gcgggcggct tctgcgcgat
    16741 cctcgcgatg ccgaacaccg atccggtgac cgactcggcg tccgtcctgc tttccttgcg
    16801 cgagagcgct gggtcctcct cgtgcagcgc cagcacgcgc cccgcgagtc gctggtactg
    16861 gagcgcctgt cgcatcacac ccgccgagcg caccggcagt ccgtcgtcgg tgaacgcggc
    16921 ggctccagcc tccgccagct ccc
//
LOCUS       DASVXS010000016         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_924164, whole genome shotgun sequence.
ACCESSION   DASVXS010000016 DASVXS010000000
VERSION     DASVXS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924164"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1327
                     /locus_tag="VF752_00865"
     CDS             407..1327
                     /locus_tag="VF752_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX8854123.1"
                     /translation="MRKLILLLVAVSSLVAATAAELARPAASATTTVTITHTGYKPKS
                     VSVSVGDSVAFTNSDTVAHTVRFKPTTGISCTRPLPLVLPPGQSATCTFRSAGSFNFS
                     DPVVKSKAFRGTVVVGAAPAVSLSATPKVVVYGRSVTLSGALASQQTGQSLQVLAQPC
                     GQASATKVATVTTTTGGAYSFQTKPLKTTTYTVKFKSSTSSAVTVNVRPRMRLGKVAP
                     HRYSLRVFAAQSFAGHYATFQRYRPALRRWVSVKRVLLRANSTGVAPTVVTAARFRSS
                     LRAGLRVRAVLSRTQVGTCYLAGRSNTIRS"
     gene            complement(1461..1533)
                     /locus_tag="VF752_00870"
     tRNA            complement(1461..1533)
                     /locus_tag="VF752_00870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1498..1500),aa:Lys,seq:ctt)
     gene            1560..>2200
                     /locus_tag="VF752_00875"
     CDS             1560..>2200
                     /locus_tag="VF752_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX8854124.1"
                     /translation="MKVFSVVGNRPQFIKAAPTSVALRERGIEEVVLHTGQHYDPELS
                     QVFFEELALAEPRYRLDLRTADTAQMQAAVAGPLGDERPDWVLVYGDTNSTLAGALAA
                     VEAKIPLAHVEAGLRSGDLSMPEERNRIETDRVAQVLFCPDERSRETLNAEAVGGHAE
                     VVGDVMADATRTFAPIARRRADVLELDSPYAVLTIHREANTQPERLRSLLEAAG"
BASE COUNT          350 a          781 c          741 g          328 t
ORIGIN      
        1 ctgaccgtcc tcctagttat ggcccgcagg gagggtcgcc tcgcgtgcac tccggcagac
       61 gcgtgccgtg gagcgaggca atcgcgcaga agcggacggc gagccgttcg gctggctact
      121 catcgccggc tgtggctcga gcgaggtgag gggccgcgcc taacccggcg gcttctacct
      181 ccgagggaat ccgctatgcg ttccccctcc cgttccaggt aggcgggaga acggcacccg
      241 tgcggctcgg ggtcggcaca cgcttcgcgg gcccaggccc gcgcgcggtg cggaaacgat
      301 cggcgccaaa gtccggtcga atttacagct tctttacgcg ggcgagggta gtggattcgc
      361 gtctggtggg atcttctcgg tagtgaaagc cagagggggg tctcacatgc gcaagctcat
      421 tctgttgctc gtcgcggtca gctcgctcgt cgcggcaacc gccgccgagc tggccaggcc
      481 cgcggccagc gctacaacca cggtgacgat cacgcacacc ggctacaagc cgaagtccgt
      541 ctcggtgagc gtcggcgaca gtgtcgcttt cacgaacagc gacaccgtcg cgcacaccgt
      601 gcgcttcaag ccgacgaccg gcatcagctg cacgcgtccg cttccgctcg tgctcccgcc
      661 ggggcagagt gcaacctgca ccttccgcag tgccggcagc ttcaatttct ccgaccccgt
      721 cgtgaagagc aaggccttcc gcggcacggt cgtggtcggt gccgcaccag cggtcagtct
      781 ctcggccacg ccgaaggtgg tcgtctacgg acgcagcgtc acgctctcgg gcgcgctcgc
      841 aagccagcag accggccagt cgctgcaggt cctcgcgcag ccgtgtgggc aggcgtcggc
      901 gacgaaggtc gccaccgtca ccacgacgac cggcggtgcc tacagcttcc aaacgaagcc
      961 gctgaagacc accacctaca cggtcaagtt caagagctcg acaagcagcg cggtcacggt
     1021 caacgtgcgg ccgcggatgc gcctcggcaa ggtcgctccg caccgctact cgctgcgagt
     1081 gttcgccgcg cagagctttg ccgggcacta cgcgaccttc cagcgctacc gccccgcgct
     1141 ccggcgctgg gtaagcgtca agcgggtgct gctgcgcgcg aactcgaccg gggtcgcacc
     1201 caccgtcgtt acagccgccc gcttccgctc gagcctcagg gctggtctgc gagtgcgggc
     1261 cgtgctcagc cgtacgcagg tcggcacctg ctacctggcc ggccggagca acacgatccg
     1321 cagttgaacc tccgcgacgg cgagccggag gtcaccgcct ccggctcgcc gtagccctca
     1381 ctcctgggca gaagcgctcc cgactaccgc gagcgctgga aggggttcct cacgcttctt
     1441 gaagctcaag cgccgggaga tgggccgtgt agggatcgaa cctacgacct tgggattaag
     1501 agtcccctgc tctaccagct gagctaacgg cccgaagcgg cgaatagtgt agccgcgcca
     1561 tgaaggtctt ctcggtggtc ggaaaccggc cccagttcat caaggcggcg ccgacctccg
     1621 tcgccctgcg ggaacgcgga atcgaggagg tcgtgctgca cacgggacag cattacgacc
     1681 ccgagctgtc gcaggtcttc ttcgaggagc tcgcgctcgc cgagccgcgc taccggctcg
     1741 acctgcgcac cgccgacacc gcgcagatgc aggcggccgt cgccggcccg ctcggcgacg
     1801 agcgccccga ctgggtgctc gtctacggcg acacgaactc cacgctcgcg ggcgccctcg
     1861 cggcggtcga ggcgaagatc ccgcttgccc acgtcgaggc cggcctgcgc agcggcgacc
     1921 tgtcgatgcc cgaggagcgg aaccggatcg agaccgaccg cgtggcgcag gtgctgttct
     1981 gtccggacga gcgctcgcgg gagacgctga acgccgaggc cgtgggcggt cacgccgagg
     2041 tcgtcggcga cgtgatggcc gacgcgaccc gcaccttcgc gccgatcgcg cggcggcgcg
     2101 cggacgtgct cgagctcgac tcgccgtacg cggtgctcac gatccaccgc gaggcgaaca
     2161 cgcagcccga acgcctgcga agcctgctcg aggcggcggg
//
LOCUS       DASVXS010000017         5486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_3720, whole genome shotgun sequence.
ACCESSION   DASVXS010000017 DASVXS010000000
VERSION     DASVXS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VF752_00880"
     CDS             <1..517
                     /locus_tag="VF752_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854125.1"
                     /translation="TAVVPHYAMSGGTLIALAADKIVLDSHTALGPVDPQLGEYPARS
                     ILEAVEENGNHEDKTLIMADVARKALEQVEVFTTSLLERNLEPKRARETARILATGTW
                     THDHPLLAPDLMALGLPVEVGIRDEERELMTLYPQPRGRTPAVEYSPGPPSPTFPQRR
                     NPQRAGRELKR"
     gene            complement(507..2264)
                     /locus_tag="VF752_00885"
     CDS             complement(507..2264)
                     /locus_tag="VF752_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8854126.1"
                     /translation="MARASPRRSDGRRDVTGARRGATDTFEETLDLYGAFPRLSDYQV
                     RALEQHGERVHTAPGEVLFREGDDYSCFWVVLEGKVATYSDYGSDEEMQIAVHGAGRF
                     LGELGVLTGQAAFLTAVACEPAELLRVPVDELRKLVTQDPVLGDLVLRAYLARRSILL
                     EVGAGLRIVGSCFSPDTRRLREFAARNRLPHRWIDLEEDQEAEALLNALGVSPQETPV
                     VIWRGSVLRNPSNAELARVIGLPSKVPQWADCDLLVVGTGPAGLAASVYGASEGLRTM
                     TFDAIATGGQAGTSPRIENYLGFPTGISGSELAERATLQAEKFGAHLHLAAEAVGLEY
                     RDGHHVVLFDDGSAVSGHALLIASGARYRKLHVARLDEFEGHSVHYAATHVEAHTCIG
                     DPVVVVGGGNSAGQSSLFLARHASLVHLLIRHEDLGRDMSRYLVNEIERDPQIHVMRC
                     TEVRELVGEHGVLNAVVAQNTRTGERWRLAARALFVFIGARPHTQWLGDQIALDDHAF
                     VLTGPAAANASNGGWDTGREALPLETSRPGVFAAGDVRAGSVKRLASAVGEGSMAVRL
                     VHEHLEKSAHWQIHAAVNA"
     gene            complement(2219..2662)
                     /gene="trxA"
                     /locus_tag="VF752_00890"
     CDS             complement(2219..2662)
                     /gene="trxA"
                     /locus_tag="VF752_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8854127.1"
                     /translation="MAATIVTCPHCGKKNRVKPVAQGVPRCANCHNLLPWMVDADSSS
                     FDDEINASVPVVVDFWAEWCGPCKMIEPALKALAEEDAGRLKIVRLNVDENPDIAARF
                     EVQGIPLLVLFRDGNEADRLVGAAPKPRLEQWLEPHLGAATGAGT"
     gene            complement(2694..3659)
                     /locus_tag="VF752_00895"
     CDS             complement(2694..3659)
                     /locus_tag="VF752_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8854128.1"
                     /translation="MPPFPKPKFAYDYDLGQEVKALRKWRDKEPGRQIPAKADGKLLL
                     ATWNVANLGVQDRSDDDCALIAEVMSWFDLVAVQEVNDDLRGISAIHSHMPDRYRLLF
                     SDASGNQERQAFLYDENKVSQLEEVGRLSIPPSQLKKIKPPGITSPFPGFDRGPYLAT
                     FASNGFEFELVNVHLFYGSDSDPIDLGRRIQETFAVAWWAKAKHDDDHSYVSNVIPLG
                     DFNLPKAAEGDRVYDALTSQGLELPPHSSQIGSAIASDNHYDQIAFFPGATHDHFTGA
                     MNVFDFDGALFHDLWETHKRSEFMAYTRFHVSDHRPLWAEFETAG"
     gene            3737..4837
                     /locus_tag="VF752_00900"
     CDS             3737..4837
                     /locus_tag="VF752_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8854129.1"
                     /translation="MARPGETIDNPSTGERITFLRTREETGGDVLEFEVVLERGARIG
                     GFPHRHPVEERFVVEEGVFATWVAGRGRRTHGPGDEVVIPPLRGHLVWNAGETEARAI
                     AEARPADDIETMFETFFGIAADGGGRLRGAPPPLHSALLAHTYELYVPLVPVALQKPL
                     LALLARAAENRGYEAVYSPRPVDPEGPSHVVPPPGGPGDLRTARTESFVASRRERVWR
                     LLTDPERYEDWVFGVSDFVDADAAWPDPGSSAKFRHGIGPASLGGSYTILHEAEPSEL
                     ELQVRIPPLIETRKRFDLTETPDGTHVTLRETPVGGALARISVIASELLVELAGTLSL
                     FALRRLLGSAGGLRARSAGSAETTAIGGAVTT"
     gene            4834..5058
                     /locus_tag="VF752_00905"
     CDS             4834..5058
                     /locus_tag="VF752_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854130.1"
                     /translation="MTDEEQLNQFEKAVEEKKRKAEEASHGRLPQNPNEGEIHGDEPD
                     IYDDSRPPDELSPRAKSTQHRKMTADKWNQ"
     gene            5081..>5486
                     /locus_tag="VF752_00910"
     CDS             5081..>5486
                     /locus_tag="VF752_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8854131.1"
                     /translation="MAGRLSLDSLEHPVVLAPLGGGPSTPALAAAVSEAGGLGFLAAG
                     YKSPDAVWDDIAALRALTDRAFGVSVFTPPPPEGDQEAVRAYAESLRGDAERLGAELG
                     TPLHDDDAWAAKLALIAEERVPVVSFTFGCPAR"
BASE COUNT          848 a         1895 c         1919 g          824 t
ORIGIN      
        1 gaccgctgtc gtgccccact acgccatgag cggcggaacg ctgatcgcgc tcgccgcgga
       61 caagatcgtg ctcgactcgc acaccgcgct cggcccggtc gacccgcagc tgggcgagta
      121 cccagcgcgg tcgatcctcg aggccgtcga ggagaacggc aaccacgagg acaagacgct
      181 gatcatggcg gacgtcgccc gcaaggcgct cgagcaggtg gaggtcttca cgacctcgct
      241 gctcgagagg aacctcgagc ccaaacgagc acgcgagacc gcccggatcc tggccaccgg
      301 cacctggacg cacgaccatc cgctgctcgc gccggacctg atggccctcg gcctaccggt
      361 cgaggtcggg atccgggacg aggagcgtga gctcatgacg ctctatccgc agccgcgcgg
      421 gcgcacgccg gcggtcgagt actcgccggg gccgccgtcc cccaccttcc cgcagcgccg
      481 aaatccccag cgcgcggggc gcgagctcaa gcgttgaccg cagcgtggat ctgccagtga
      541 gccgactttt ccaggtgctc gtgcacgagc cgcaccgcca tggaaccctc gccgacggcg
      601 gaggccagcc gcttcacgga tccggcgcgc acgtcgcctg ccgcgaacac cccaggtcgg
      661 ctcgtctcga gcggtagcgc ctcgcgtccg gtgtcccagc cgccattcga tgcgttagcg
      721 gcggcggggc cggtcaggac gaaagcgtgg tcgtcgagcg cgatctgatc gccgagccac
      781 tgggtgtgcg ggcgcgctcc gatgaagacg aacagcgcac gcgccgcgag cctccaccgc
      841 tcgcccgtcc gcgtgttctg cgcaaccacc gcgttcagca ctccgtgctc gcccaccagt
      901 tcgcggactt cggtgcagcg catcacgtgg atctgcgggt cgcgctcaat ctcgttcacc
      961 agatagcgcg acatgtcgcg tccgaggtcc tcgtgccgga tcagcaggtg aaccagagag
     1021 gcgtgcctcg cgaggaacag cgaggactgc ccggctgagt tgccaccgcc gacgaccacc
     1081 acggggtcgc cgatgcaggt gtgcgcctcc acgtgcgtgg ccgcgtagtg cacgctgtgc
     1141 ccctcgaact catcgaggcg cgccacgtgt agcttccggt agcgggcgcc ggatgcgatc
     1201 aggagagcgt ggcctgagac ggcgcttccg tcgtcgaaca gcacgacgtg atgcccgtcg
     1261 cggtactcga gcccgaccgc ctccgcggcg agatgcaggt gggcaccgaa cttctccgcc
     1321 tgcagcgtcg cgcgctccgc cagttcggag cccgagatgc cggtggggaa gcccaggtag
     1381 ttctcgatgc gcggcgacgt gccggcctgg ccgccggtcg cgatcgcgtc gaaggtcatc
     1441 gtcctgagcc cttcggaggc cccgtagacg gaggcggcga ggcctgccgg gccggtaccc
     1501 accacgagca ggtcgcagtc ggcccactgc ggaaccttcg acggaagccc gatcacacgc
     1561 gccagctcgg cgttgctcgg gttgcgcagc acgctgccgc gccagatcac cacgggcgtc
     1621 tcctgtggcg acacgccgag cgcgttgaga agcgcctcgg cctcctggtc ctcctccagg
     1681 tcgatccagc ggtgcggcag ccgattccgc gcggcgaact cgcgtagccg gcgcgtgtcg
     1741 ggggagaagc acgagcccac gattcgaagc ccggcaccaa cctcgagcag tatcgagcgc
     1801 cgcgcgagat aggcacgcag gacgaggtcg cccagaacgg gatcttgggt gaccagcttt
     1861 cgcagctcat cgaccggaac gcgcaagagc tccgcgggct cgcacgccac ggccgtaaga
     1921 aacgccgcct ggccggtgag gacgccgagc tcgccgagga agcggccggc gccatgaacc
     1981 gcgatctgca tctcctcatc gctgccgtag tccgagtacg tggcgacttt gccctcgagc
     2041 accacccaga agcagctgta gtcgtcgccc tccctgaaga gaacctctcc gggcgcggta
     2101 tgcacccgct cgccgtgctg ctccagcgcg cggacctgat agtcgctcag ccgcggaaac
     2161 gcgccgtaga ggtcgagggt ctcctcgaag gtgtccgtcg cgccgcggcg ggcgccggtc
     2221 acgtcccggc gcccgtcgct gcgccgaggt gaggctcgag ccactgctcg agtctcggtt
     2281 tcggggcggc gcccacgagc cggtcggctt cgttcccgtc ccggaacagg acgagcaggg
     2341 ggatgccctg cacctcgaag cgagccgcga tgtccgggtt ctcgtcgacg ttcagccgga
     2401 cgatcttgag cctgccggcg tcctcttccg cgagcgcctt caaggcgggc tcgatcatct
     2461 tgcaggggcc gcaccactcg gcccagaagt cgacgacgac gggcacggac gcgttgatct
     2521 cgtcatcgaa gctgctcgag tcagcgtcca ccatccacgg caggaggttg tggcagttcg
     2581 cgcagcgggg cacgccctgg gcgacgggct tcacccggtt cttcttgccg cagtgcgggc
     2641 aggtgacgat cgtggccgcc acaaggttga tcctaggtcg cccggcgcgt ttctcaaccc
     2701 gctgtctcga actccgccca gagcgggcgg tggtccgaga cgtggaagcg cgtgtacgcc
     2761 atgaactcgc ttcgcttgtg cgtctcccag aggtcgtgga agagcgcgcc atcgaagtcg
     2821 aacacgttca tcgcgccggt gaagtggtcg tgcgtggctc cagggaagaa ggcgatctgg
     2881 tcgtagtggt tgtcggacgc gatcgccgaa ccgatctgcg aggagtgcgg aggcagctcg
     2941 agcccctgtg aggtgagcgc gtcgtatacg cggtcgccct cggcagcctt cggcagattg
     3001 aaatcgccga gcgggatcac gttcgacacg taggagtggt cgtcgtcgtg cttcgccttc
     3061 gcccaccagg cgaccgcaaa ggtctcctgg attcgcctgc cgaggtcgat cgggtcgctg
     3121 tccgagccgt agaagagatg cacgttcacg agctcgaact cgaacccgtt agacgcgaac
     3181 gtggcgaggt acgggccgcg gtcgaagcca ggaaacggcg acgtgatccc cggcggcttg
     3241 atcttcttca gctggctcgg tggaatcgag aggcggccga cctcctcgag ctgggagacc
     3301 ttgttctcgt cgtagagaaa ggcctgccgt tcctggttgc ccgacgcgtc cgagaacagc
     3361 agccggtagc ggtccggcat gtgcgagtgg attgcgctta tgccgcgcag gtcgtcgttc
     3421 acctcctgaa cggccacgag gtcgaaccag ctcatcacct ccgcgatcag cgcgcagtcg
     3481 tcgtcggagc ggtcttgcac gccgaggttc gccacgttcc acgtggccag gagcagcttg
     3541 ccgtccgcct tggccgggat ctgccggccc ggctccttgt cgcgccactt gcggagcgcc
     3601 ttgacctcct gcccaaggtc gtagtcgtac gcgaacttcg gcttcgggaa tggcggcatg
     3661 gtccacttct acacccgggc gccggcagcg gcaaagccct gtttcgtgag aagcgtcacg
     3721 ggtagtcggt gccttcatgg cccggcccgg cgagacgatc gacaacccct cgacgggaga
     3781 acgcatcacg ttcctccgca cgcgggagga gaccggcggg gacgtgctcg agttcgaggt
     3841 ggtgctcgag cgcggcgcgc gtatcggcgg cttcccgcac agacatccgg tcgaggagcg
     3901 gttcgtggtg gaggagggcg tgttcgccac ctgggtcgct ggccggggcc gtcgcacgca
     3961 cgggccgggc gacgaagtcg tgatcccgcc gctgcgcggg cacctcgtgt ggaacgccgg
     4021 cgagacggag gcacgtgcga tcgccgaggc gcgtcctgcc gacgacatcg agacgatgtt
     4081 cgagaccttc ttcggcatcg cggccgacgg cggtggccgc ttgagaggtg cgccgccgcc
     4141 gctgcactcg gcgctgctgg cccacaccta cgagctctac gtgccgctcg tgccggtcgc
     4201 cctgcagaag ccgctgctcg ctctgctggc gcgcgcagcg gagaaccgtg ggtacgaggc
     4261 cgtgtactcg ccgcggccgg tcgatcccga gggcccgtcg cacgtggtcc cgccgcccgg
     4321 cggtcccggt gacctgcgca ccgcccgcac cgagagcttc gtcgccagcc ggcgcgagcg
     4381 cgtatggcgg ctgctcaccg accccgagcg ctacgaggac tgggtcttcg gggtctccga
     4441 cttcgtggac gccgatgcgg cctggccaga cccgggcagc tcggcgaagt tccggcacgg
     4501 gatcggacct gcctcgctcg gcggctcgta cacgatcctg cacgaggccg agccgagcga
     4561 gctcgagctc caggtgcgga tccccccgct gatcgagacc cgcaagcgct tcgacctgac
     4621 cgagacgccc gacggcacgc acgtcacgct gcgggagaca ccggtgggcg gcgcgctggc
     4681 gcgaatctcc gtgatcgcga gcgagctgct ggtggagcta gccggcacgc tcagcctgtt
     4741 tgcgcttcgg cgcctgttgg gctccgcggg cggtttgcgg gcgcgctccg cgggtagcgc
     4801 cgagacgacg gctatcggag gagcagtcac cacatgaccg acgaagagca gctcaatcag
     4861 tttgagaagg cggtcgagga gaagaagcgc aaggccgagg aggcctcgca tgggcggctg
     4921 ccccagaatc cgaacgaagg cgagatccac ggggatgagc cggacatcta cgacgacagc
     4981 cggccgcccg acgagctgag tccgcgtgcg aagagcacgc agcaccgcaa gatgaccgcg
     5041 gacaagtgga accagtagcg gtgccggtag cgtgcccgcc gtggcgggcc gactgagcct
     5101 cgacagcctg gagcaccccg tcgtgctggc gccgctcggc ggcgggccct ctacgcctgc
     5161 gctcgcggcc gcggtgtccg aagcgggcgg gctcggcttc ctcgccgccg gctacaagtc
     5221 gccggacgcc gtgtgggacg acatcgccgc gctgcgcgcg ctcaccgatc gcgcgttcgg
     5281 cgtgagcgtg ttcacgccgc caccgccgga gggcgaccag gaggccgtgc gcgcctacgc
     5341 ggagtcgctg cgcggcgacg ccgagcgcct cggcgccgag ctcggcacgc cgctgcacga
     5401 cgacgatgcc tgggccgcca agctggcgct gatcgcggag gagcgggtgc ccgtggtgtc
     5461 gttcacattc ggctgcccgg cgcgca
//
LOCUS       DASVXS010000018         9502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_287550, whole genome shotgun sequence.
ACCESSION   DASVXS010000018 DASVXS010000000
VERSION     DASVXS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287550"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3760)
                     /gene="gltB"
                     /locus_tag="VF752_00915"
     CDS             complement(<1..3760)
                     /gene="gltB"
                     /locus_tag="VF752_00915"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX8854132.1"
                     /translation="MPLAKRPEAVGLYDPRFEHDACGVGAVADLSGEPRHETVVRALD
                     VLDRLEHRGATGPEIDTGDGAGILLQLPDEFLRAVAGVELPEPGRYAAGMVFLPRDDA
                     RRAEIESLIERTVTSEGIQPLGWRDVPVDSSIPGPSARDVEPVIRQVFIATELDDQDA
                     FERKLYVARRQIEQNAGEDVAIPSFSSRTLVYKGMLTSPQLRRYYPDLNDARVKSALA
                     LVHSRFSTNTFPSWELAHPYRLIAHNGEINTLRGNVNWMAARESQLASELFGDDIEKI
                     KPVIRPGGSDSATFDNVLELLVLGGRSLPHALMMMIPEAWEGREDMPEEVRNFYAYES
                     CLMEPWDGPAAVAFTDGRVIGATLDRNGLRPGRWQLTRDGIVVLASETGVLEYEPEEV
                     VTKGRLQPGKIFLVDVEQGRIVEDGEVKHEVASQKPYGRWYGERTVHLDDLPDVAPEE
                     LPADPLGTRQQLFGYTQEDLRVTLAQMGGAKAEEPTGSMGNDFSLAVLSDRSPLLYSY
                     FKQLFAQVTNPPIDPIREKIVMSLSTSVGPEGNLLDETAEHAHQLIIPQPLLTSSELE
                     KLRQVDHHVFTAATLDATWPVSEGVEGLERALARLCRESDEHIANGDNILIISDRAAG
                     ADRAPIPALLALAGVHHHLVREHTRLRTGLVVESAEPREVHHIATLLGYGASAVNPYL
                     AFETLYEMFDRGLLPGVEGADVAERNMIKAVGKGLLKTISKMGISTVRSYSGAQIFEA
                     VGLEPELIDKYFTGTTSRIGGIGLDVIATETLARHRRAYAAEKPAEAERLANGGVHAW
                     RRDGEHHQWNPETIATVQHAVRADGREAYEEFSRRVNEESSRKATIRGLLKLRPQPEP
                     IPLDEIEPAAEIVKRFSTGAMSLGSISTEAHETLAIAMNRLGGKSNTGEGGEDPRRFK
                     PDPNGDNRRSAIKQVASGRFGVTAHYLVNADELQIKMAQGAKPGEGGQLPGHKVDEYI
                     ASVRHSTPGVGLISPPPHHDIYSIEDLKQLIFDLRCANPSARISVKLVSEVGVGTVAA
                     GVAKANSDHVVIAGHDGGTGASPVSSIHHAGVPWEIGLAETQQTLVLNRLRDRIIVQT
                     DGQLKTGRDVVVAGLLGAEEFGFATAPLIAMGCIMMRACHLNTCPVGIATQDPELRKR
                     FAGKPEHVVNYFFLLAEEVRELMAQLGVRTFDELVGRTDLLEADEAIEHWKARGVDLS
                     HILAPIDMPPEVARRKVRPQDPVLDDHLDHALIEVAAPALERGERVAAG"
     gene            3942..4790
                     /locus_tag="VF752_00920"
     CDS             3942..4790
                     /locus_tag="VF752_00920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX8854133.1"
                     /translation="MKLGIAMFPTDNAVRPDELARLVEERGFESLWFAEHTHIPVSRE
                     TPYAGGGELPDRYSRTHDPFVALTAAAAATSALLIGTGICLVIERDPIVTAKEVASVD
                     QLSGGRFLFGIGGGWNREEMVNHGTDPDHRFRLMRERVEAMKAIWAEDEAEYHGRYVN
                     FDPIWSWPKPVQEPHPPIVVGGNGENVLKRVVSYGEEWMPNRLLDEPERLGERIVDLQ
                     RMAEDAGRGEIPVTVSGIRPDPGLIERHEAIGVSRATIYIPPVDRDEAESRLDHLAQK
                     LGPVRT"
     gene            4924..6135
                     /locus_tag="VF752_00925"
     CDS             4924..6135
                     /locus_tag="VF752_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8854134.1"
                     /translation="MSSSVDTATDIRPFQVDIPDEALQDLRRRIAGTQWPEKETVGDE
                     SQGVQLETIQRLARYWATEYDWRKCEAKLKALPQFITEIDGLDIHFIHVLSQHEDALP
                     LIVNHGWPGSIIEQLKIIDPLTNPTAHGASASDAFHVVIPSMPGYGFSGKPTSTGWGP
                     ERMGQAWAELMKRLGYTRYVAQGGDWGAFVVDQMGLQAPDGLLAIHSNMPATVPDDVD
                     KALQAGEPPPSGLSTEERRAYEQLVRTFKQVEYAHMMAARPQTLYGIADSPVGLAAWL
                     LDHNDADGQPAAAVTAGLERTESATGELTRDEVLDNITLYWLTNTGVSASRLYWEYKG
                     GFFNTKGVSIPTAITVFPGEQYQAPRSWADRAYPNLIYYNEVDKGGHFAAWEQPELFS
                     QEIRAAFKSVR"
     gene            complement(6379..6751)
                     /gene="rnpB"
                     /locus_tag="VF752_00930"
     ncRNA           complement(6379..6751)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF752_00930"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7139..7750)
                     /gene="lexA"
                     /locus_tag="VF752_00935"
     CDS             complement(7139..7750)
                     /gene="lexA"
                     /locus_tag="VF752_00935"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX8854135.1"
                     /translation="MDLKLTKRQQEIFDFIKRYSAKHGYPPTVRDIGKAIGLTSSSTV
                     HAHLANLEKLGLLRRDPTKPRALELLNKAKEAVTGSAGLPLVGRVAAGEPILAEENVE
                     DYVDVPGAIGGDEADYLLRVAGDSMKDVGILEGDFVVVRSQETANDGEIVVALVGEEA
                     TVKRFYREADHIRLQPENDAYEPIRTREAQVLGRVVGVFRRVT"
     gene            7884..7969
                     /locus_tag="VF752_00940"
     tRNA            7884..7969
                     /locus_tag="VF752_00940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7918..7920,aa:Leu,seq:gag)
     gene            complement(8321..8860)
                     /locus_tag="VF752_00945"
     CDS             complement(8321..8860)
                     /locus_tag="VF752_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854136.1"
                     /translation="MSANPEAIRAPHATRRRSVVARTALYLGALAVVATGVAHAQQYY
                     GNNYSTIPTIGTLFFLNFVAAVTLAVGLVVPLRRVIPRYAEAVRALFAIGGICLGVLS
                     LIALFVSESSGLFGFVENGYRTAIVLAIIVEAAAVVFLAVYLAADAAGPRQLRDLAAK
                     AVTRTRAVGPRHRGSEERA"
     gene            complement(8895..>9502)
                     /locus_tag="VF752_00950"
     CDS             complement(8895..>9502)
                     /locus_tag="VF752_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854137.1"
                     /translation="MRRKLVTLTWTRVAAGVVAIAVLAAGLVVAFVGSGDSSRGAASG
                     SFAWLHPASPPAGWKVARTARGAALAYPPGWTPIESDRGTASVALLGSGGRIAGYLNA
                     TPKQGAETLANWSRFRPQHNSNEGSRDEHLIASTTDARFRSAHGACVIDSYSTSRAAY
                     REIACLAAGPRSTAVVVAAAPEDLWAREAATLERAVSSFVP"
BASE COUNT         1488 a         3204 c         3256 g         1554 t
ORIGIN      
        1 tgccggccgc cacgcgctcg ccgcgctcga gcgccggcgc cgcgacttcg atcagcgcgt
       61 ggtcgaggtg gtcgtcgagg acggggtcct gcggccgaac cttgcggcgg gccacctcgg
      121 gcggcatgtc gatcggggcg aggatgtgcg agaggtcgac gccgcgcgcc ttccagtgct
      181 cgatcgcctc gtcggcctcg agcaggtccg tgcggccaac gagctcgtcg aacgtgcgca
      241 cgccgagctg tgccatcagc tcgcgaacct cctctgccag gaggaagaag tagttcacga
      301 cgtgctcggg cttgccggcg aagcgcttgc gcagctcggg gtcctgggtg gcgatgccga
      361 ccgggcacgt gttgaggtgg caggcgcgca tcatgatgca gcccatcgct atcagcggcg
      421 cggttgcgaa gccgaactcc tcggcgccga gcagcccggc aaccaccacg tcgcggcccg
      481 tcttcagctg accgtcggtc tgcacgatga tgcggtcgcg caggcgattc agcacgagcg
      541 tctgctgcgt ctccgccagg ccgatctccc acggcacgcc ggcgtggtgg atcgacgaga
      601 cgggagacgc gccggtgccg ccgtcgtgcc ctgcgatgac cacgtggtcc gagttggctt
      661 tcgcgacgcc cgcggcgacc gtgccgacgc cgacctcgga cacgagcttc acgctgatgc
      721 gcgccgacgg gttggcgcag cgcaggtcga agatgagctg cttgagatct tcgatcgagt
      781 agatgtcgtg gtgtggcggc ggtgagatga ggccgacgcc aggcgtcgag tggcgcacgc
      841 tcgcgatgta ctcgtccacc ttgtggccgg gcagctgacc gccctcgccg ggcttcgcgc
      901 cctgcgccat cttgatctgg agctcgtcgg cgttcacgag gtagtgcgcg gtcacgccga
      961 agcgcccgga cgccacctgc ttgatcgccg atcggcggtt gtcaccgttc gggtccggct
     1021 tgaaacgacg cgggtcctcc ccgccctcgc cggtgttcga cttgccgccg aggcggttca
     1081 tcgcgatagc gagcgtctcg tgcgcctccg tggagattga gccgagcgac atggcgccgg
     1141 tcgagaagcg cttcacgatc tcggcggcgg gttcgatctc gtcgagcggg atcggctcgg
     1201 gctgcggccg cagcttcagg aggccgcgga tcgtcgcctt gcgcgaggac tcttcgttca
     1261 ccctgcgcga gaactcctcg taggcctcgc gtccgtccgc gcgcacggcg tgctgcacgg
     1321 tcgcgatcgt ctccgggttc cactggtggt gctcgccgtc gcggcgccat gcgtggacgc
     1381 cgccgttcgc cagccgctcg gcctcggccg gcttctccgc ggcgtacgcc ctgcggtggc
     1441 gcgcgagcgt ctcggtcgcg atcacgtcga ggccgatacc gccgatgcgc gaggtcgtcc
     1501 ccgtgaagta cttgtctatc agctcgggct cgagcccgac cgcctcgaag atctgcgcgc
     1561 ccgagtacga gcggacagtg ctgatgccca tcttcgagat cgtcttgagc aagcccttgc
     1621 cgaccgcctt gatcatgttg cgctccgcca cgtcggcgcc ttcgacgccg ggcagcaggc
     1681 cgcggtcgaa catctcgtag agcgtctcga acgcgaggta ggggttcacc gcggaagcgc
     1741 cgtagccgag cagcgtggcg atgtggtgca cctcgcgcgg ctcggccgac tcgaccacca
     1801 gcccggtgcg caagcgcgtg tgctcgcgca cgaggtggtg gtgcacgccg gcgagcgcga
     1861 gtagcgccgg gatcggggcg cggtcggcgc cggcggcgcg gtcactgatg atgaggatgt
     1921 tgtcgccgtt agcgatgtgc tcgtcggact cgcggcagag tcgtgcgagc gcgcgctcga
     1981 gcccctcgac gccttcgctc accggccacg tcgcgtcgag cgtggccgcc gtaaacacgt
     2041 ggtggtcgac ctggcgcagc ttctcgagct cgctcgaggt gaggagcggc tgggggatga
     2101 tcagctgatg ggcgtgctcg gcggtctcgt cgagcaggtt gccctcgggc cccaccgacg
     2161 tggagaggct catcacgatc ttctcgcgga tcggatcgat cggcggattc gtgacctgcg
     2221 cgaagagctg cttgaagtag ctgtagagca gcggcgaacg atctgagagg accgccagcg
     2281 agaagtcgtt gcccatcgag ccggtcggct cctcggcctt ggcgccaccc atctgcgcga
     2341 gcgtgacccg caggtcctcc tgcgtgtagc cgaagagctg ctggcgggtg ccgagcgggt
     2401 cggcgggtag ctcctcgggc gcgacgtccg gcaggtcgtc gaggtgcacg gtgcgctcgc
     2461 cgtaccagcg gccatacggc ttctggctgg cgacctcgtg cttcacctcg ccgtcctcga
     2521 cgatgcggcc ctgctccacg tcgaccagga agatcttgcc gggctgcagg cgtcccttcg
     2581 tgaccacttc ctcgggctcg tactcgagca cgccggtctc ggacgccagc accacgatgc
     2641 cgtcgcgcgt gagctgccag cggccgggtc gcaagccgtt gcggtcgagc gtcgcgccga
     2701 tcacgcggcc gtcggtgaac gcgaccgccg ccgggccgtc ccacggctcc atcaggcagg
     2761 actcgtacgc atagaagttg cgcacctctt cgggcatgtc ctcgcggccc tcccaggcct
     2821 cggggatcat catcatcagc gcgtgcggca gcgagcggcc gccgagcacg agcagctcga
     2881 gcacgttgtc gaacgtggcc gagtccgagc cgcccgggcg gatgaccggc ttgatcttct
     2941 cgatgtcgtc gccgaacagc tcgctcgcaa gctgcgactc gcgcgcggcc atccagttca
     3001 cgttgccgcg cagcgtgttg atctcgccgt tgtgggcgat caggcggtag gggtgcgcaa
     3061 gctcccagct cgggaaggtg ttcgtggaga agcgcgagtg cacgagcgcg agcgcacttt
     3121 tcacgcgcgc gtcattcagg tcggggtagt agcggcggag ctgcgggctc gtgagcatgc
     3181 ccttgtagac gagcgtgcgc gaagagaacg aggggatcgc gacgtcctcg ccggcgttct
     3241 gctcgatctg gcggcgcgcg acgtagagct tgcgctcgaa cgcgtcctgg tcgtcgagct
     3301 cggtggcgat gaacacttgg cgaatgacgg gctcgacgtc gcgggcactc ggtccgggga
     3361 tcgacgaatc gaccggcacg tcgcgccagc cgagcggctg gatgccctca ctcgtgacgg
     3421 tgcgctcgat gaggctctcg atctccgcgc gccgcgcgtc gtcgcgcggg aggaacacca
     3481 tgccggcggc gtagcgcccg ggctcgggca gctccacgcc ggcgaccgcg cgcaggaact
     3541 cgtcgggcag ctgaaggagg attcccgcgc cgtcccccgt atcgatctca ggacccgtcg
     3601 cgccgcggtg ctcgagcctg tcgagcacgt cgagcgctcg aacgacggtc tcgtgcctcg
     3661 gctcgccgga gaggtcggcg actgcgccga cgccacacgc gtcgtgctcg aatctagggt
     3721 cgtagaggcc gacggcctca ggtcgctttg cgagtggcat aagtcttcgt agggagcggc
     3781 cttcggccgg gcggcggcca aagcgaacgc aggatcttac cggtcaggcg gccggaaacc
     3841 gttccttggt ccggggtgcc gccttcgcgc cgcattgaga gtgccgcacg ttccggcgat
     3901 cgtcaatgag ccgcccaaca cgcctctgta cgctcccgcc ggtgaagctc ggcatcgcga
     3961 tgtttcccac cgacaacgcc gtccgcccgg atgagctagc ccggctggtg gaggaacgcg
     4021 gcttcgagtc tctttggttc gccgagcaca ctcacatccc cgtgagccgg gagacgccat
     4081 acgccggcgg cggcgagctc cccgataggt acagccgcac gcatgacccg ttcgtcgcgc
     4141 tgacggccgc tgcggccgcg acgtcggcgc tgctcatcgg aaccggcatc tgcctcgtca
     4201 tcgagcgcga cccgatcgtc accgccaaag aggtcgcgag cgtcgatcag ctttccggcg
     4261 gccgcttcct gttcggcatc ggtggcggct ggaaccgcga ggagatggtc aaccacggca
     4321 ctgaccccga tcaccgcttc cgcctgatgc gcgagcgcgt cgaggcgatg aaggcgatct
     4381 gggccgaaga tgaggccgag taccacgggc gctacgtcaa cttcgacccg atctggtcgt
     4441 ggccgaagcc ggtccaggag ccgcacccgc cgatcgtggt cggtggcaac ggcgagaacg
     4501 tgctgaagcg agtcgtctcg tacggcgagg agtggatgcc gaaccgcctg ctcgacgagc
     4561 ccgagcgcct cggcgagcga atcgtcgatc ttcagcgcat ggccgaggat gccggccgcg
     4621 gagagattcc cgtgacggtc tccgggatcc ggcccgaccc ggggctgatc gagcgccacg
     4681 aagcgatcgg ggtctcgcgc gcgactatct acatacctcc cgtcgaccgc gacgaggcgg
     4741 agagccgcct cgaccacctc gcccagaagc tcgggccggt acgcacctag cggacctcat
     4801 ctgacgcgct cgccggcgcg tacgtggggc agcacctata ccccgatccc cgcagcgacg
     4861 aggactagga gcaaaggccc ggacgtcact cgggcccggc cccaccgacc aacggaggtc
     4921 gtcatgagca gcagcgtcga caccgcgacc gacatccgtc ccttccaggt cgacatccca
     4981 gacgaggcac tgcaagacct gcgtcgacgc atcgcgggca cgcagtggcc cgagaaggag
     5041 accgtgggtg acgaatcgca gggcgtgcag ctcgagacga ttcagaggct cgcgcgctat
     5101 tgggcgacgg agtacgactg gcgcaagtgc gaggcgaagc tgaaggccct accgcagttc
     5161 atcaccgaga tcgatgggct cgacattcac ttcattcatg tcctttcgca acatgaagac
     5221 gcgttgccgc tcatcgtcaa tcacggatgg cccggctcga tcatcgagca actgaagatc
     5281 atcgatccac tgacgaatcc cacggcacat ggcgcgagcg catcggacgc gttccatgtg
     5341 gtgattccgt cgatgccggg ctacgggttc tcaggcaagc cgacgagcac cggttggggc
     5401 cccgagcgca tgggccaagc ctgggcggaa ctgatgaagc gcctcggcta cacgcgctac
     5461 gtcgcccagg gcggcgactg gggcgcgttc gtcgtcgacc agatgggcct gcaggcacct
     5521 gacggattgc tagccatcca cagcaacatg ccggccaccg ttccggatga cgtcgacaag
     5581 gctctccagg cgggcgagcc gccgccatcc ggtctctcga ctgaggagcg ccgcgcctat
     5641 gagcagctcg tcagaacgtt caagcaggtc gagtacgcac acatgatggc ggcgcgcccg
     5701 cagacccttt acggcattgc ggattcaccc gtcggcctgg ccgcctggct tctcgaccac
     5761 aacgacgccg acggccagcc ggccgctgcg gtcaccgctg ggctcgagcg gactgagagc
     5821 gccacgggcg agctgacgcg tgacgaggtg ctcgacaaca tcacgctcta ttggctaacg
     5881 aacaccgggg tctctgcgtc tcgcctctat tgggaataca agggtgggtt cttcaacacc
     5941 aagggtgtct ccatccccac tgcgataacc gtgtttcccg gtgagcagta tcaagcgccg
     6001 cgtagctggg cggaccgggc ttatccgaac ctcatctact acaacgaggt cgacaagggc
     6061 ggtcacttcg ccgcgtggga gcagccggag ctattcagcc aggaaatccg ggcggcgttc
     6121 aagtcggttc ggtagcgctg ggaacggaag ccacgagcgg aatatccgcg ctcgccgcaa
     6181 cagaagcgag caaggaaggc ggcgcgcggc cgggccgacg cactcacgaa cgccgcgccc
     6241 gcgggtggcg cgcgacgggg tgtctgccga ggaaccgcag tcccggtccg caggcagaca
     6301 cccggagcag cgacaggacc cgcagccgac cacgtagcgg gcccaagaac gtagaaaggc
     6361 cccgccgaag cggggccttc ccttagctgg cctgtaagcc ggattctgtc gcgagcaacc
     6421 atccatctcc cccggtctgc gaccggggtg ggctttcgcc ctgcggccta cctgggactc
     6481 agcgggcagc gtcgtcgtcc ctgcttggcc ttgcaccggg tggggtttgc ctagccgccg
     6541 tgtcgccacg acgctggtgc gctcttaccg caccgtttca cccttgcctg caacggacct
     6601 cacgtgaggg cccgccacag gcggtttgtt tctgtggcac tttcccgcgg gtttccccgg
     6661 gtcagtctcc tgaccaccct gccctgcggt gtccggactt tcctcgagaa ccggttttct
     6721 ctccggtccc cgcggctgct cagccagccc tacaagtcta gattcggcac ctgcgcgcgg
     6781 ctactcgcaa gagtgctttc acgacaccgt cgcgccgcag tttccgcagt gcccatcgtt
     6841 cccgcgccgc tcgagcgggc cgcggcagac ggggcagtcg gccgctccag tggcaaggag
     6901 accctcccat acgctggaga tccgctgctc caacgtgagt ctcctgccac cggaacgaac
     6961 gcctctcgcg accgagtcgc cgaacagcgg ctcggggcga agccttgttt caagcgttgc
     7021 tgtactcatt tttcgctccc ttcatgagcc gaaaaccgag ctatgcaagg acgcagggtc
     7081 gaagcggtat gccaataccg cgagacccga ggacgcagca gagcgacggt ttgcaggctc
     7141 atgtaaccct tcggaagacg cccacaacgc gaccgaggac ttgcgcctcg cgcgtgcgaa
     7201 tgggctcgta cgcatcgttc tccggctgga gccggatgtg gtcggcctcg cggtagaagc
     7261 gcttcacggt cgcctcctcg cccacgagcg ccacgacgat ctcgccgtcg ttggctgtct
     7321 cctgcgaacg cacgaccacg aagtcgccct cgaggatgcc gacgtccttc atcgagtcgc
     7381 cggcgacgcg gagcagatag tccgcctcat cgccgccgat ggcgcccggc acgtcgacgt
     7441 agtcctccac gttctcctcg gccaggatcg gctcgccggc agccacgcgg ccgacaagcg
     7501 gcaggccggc ggagccggtg acggcctcct tcgccttgtt caggagttcg agcgcgcgcg
     7561 gcttggtcgg atcgcggcga agaagcccga gcttctcgag gttcgccagg tgcgcgtgca
     7621 ccgtggacga cgacgtgagc ccgatcgcct taccgatgtc gcggacggtc ggcgggtatc
     7681 cgtgcttcgc cgaataccgc ttgatgaagt cgaagatctc ctgctggcgc ttggtcagct
     7741 tcaggtccat cggttctcca tgccggggcc gttgagatgc tcaattgcga acatgtgttc
     7801 gtagactagc cacccccgcg gacggcatcc agtcgacgtg ggcatttgca cccccagggc
     7861 tatactcagc gccgaattcg cctgcgcctg tggcggaatt ggtagacgcg caaggttgag
     7921 ggccttgtcc ggggtatccc gggtgggagt tcgagtctcc ctgggcgcac tactcggttg
     7981 tcgagcgtgt gcagagtcgg ccgggggctg gctgtcgatt cgcgagctag tagccactgc
     8041 tgccccccga ggtagacgtc gagctcgagc agtcgcgcac gcaccggagc acacgctccc
     8101 cgttcccgag tccttctgga acgggtagag cgtgcggcct cgggagtcga ccaggatctt
     8161 cccaagttgc gggctggcga cgctgaccgt ggcggcctgt ccggtcgagg tcttgggcgg
     8221 gcggtaacgc cgctcccgcc tcacccacat gcggtcacgg cgacagaact gaacgcgacc
     8281 gcgccgagtg gcatgacgaa aacgagaggt ctgttccagg tcatgctcgc tcctccgagc
     8341 cgcggtgcct cgggccgacc gctcgtgttc tggtcacggc cttcgctgcc aggtcgcgta
     8401 gttgccgagg gcccgcagcg tccgcggcga ggtagaccgc gaggaagacg acggccgccg
     8461 cctcaacgat gattgccagc acgatcgcgg tgcggtagcc gttctccacg aatccgaaga
     8521 ggccggacga ttccgagacg aacagggcga tcagcgagag cacacccagg cagatgccgc
     8581 caatcgcgaa cagcgcacgt accgcctccg cgtaacgagg gatgacgcgg cggaggggta
     8641 cgaccagtcc gacagccagg gtgacggcgg ccacgaagtt caggaagaac agggtgccga
     8701 tcgtcgggat ggtcgagtag ttgttgccgt agtactgctg ggcgtgggcg acaccggtcg
     8761 cgacgacggc gagcgcgccg aggtatagcg cggtccgcgc gacgaccgac cggcgtcgcg
     8821 ttgcgtgcgg cgcgcgtatt gcttcgggat tggcactcat agagactcct ttgcgtgttg
     8881 ggcattgccg tggctcacgg gacgaagctc gagaccgccc gctccaatgt cgccgcctct
     8941 cgggcccaca ggtcttccgg cgccgcggcg acgaccaccg cggtcgatcg cggccccgcg
     9001 gcaagacagg cgatctcgcg gtaggccgct ctcgaggtgg agtaggagtc gatgacgcac
     9061 gccccgtgcg cggagcgaaa gcgcgcgtct gtggtcgagg cgatcaggtg ctcgtcgcgg
     9121 gagccctcgt tgctgttgtg ctgcggccgg aaccggctcc agttcgcgag cgtctcggcg
     9181 ccctgcttcg gggtcgcgtt gaggtagccg gcgatccggc cgccgctgcc gaggagggcc
     9241 accgaagccg taccgcggtc gctttcgatc ggggtccatc cgggcggata cgcgagcgcg
     9301 gctccgcgtg ccgtacgagc gaccttccat cccgccggcg gggacgcggg gtgcagccag
     9361 gcgaacgacc ccgacgcggc gccgcggctc gagtcgccgc tgcccacgaa ggccacgacc
     9421 agccccgcgg cgagcacggc tatggcgacc acgcctgcgg ccactcgcgt ccaagtcagc
     9481 gtcacgagct ttcgcctcat ac
//
LOCUS       DASVXS010000019         7174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_287801, whole genome shotgun sequence.
ACCESSION   DASVXS010000019 DASVXS010000000
VERSION     DASVXS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287801"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /gene="nadA"
                     /locus_tag="VF752_00955"
     CDS             <1..623
                     /gene="nadA"
                     /locus_tag="VF752_00955"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX8854138.1"
                     /translation="AEVKAETDYCCTSSNAVQVVEHIRREHGDDVEILFGPDMFLGAY
                     VEKVTGKRMSVWMGECHVHAGIRPSDIAQVREANPGAEFLIHPECGCSTSVMEYVASG
                     DVDPDHTHMLSTGGMLDFARYSDADTFIVATETGMLHPLEQENPGKTFVPANRAAVCQ
                     FMKMITLPKLRDALRDGGEAYTVKVDPELAERARLPIERMVAISGA"
     gene            complement(620..1225)
                     /locus_tag="VF752_00960"
     CDS             complement(620..1225)
                     /locus_tag="VF752_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8854139.1"
                     /translation="MTTAPRATQAQRSARTRSALLESAARGLSRYGYGKLRLEEVARD
                     AGYTRGALYHQFKDKDELVLAALEWVDETWRAEVGTAIERASDPASALIALARAHAVF
                     CRRDVARFEIALRLEFSSRDHPVAREVERISETLVKRCARLINAGRKAGTIPPGPPAR
                     AVALAYIGALEGTVIALAGQAPHDEILAARAVAGVLGLPTP"
     gene            1271..1879
                     /locus_tag="VF752_00965"
     CDS             1271..1879
                     /locus_tag="VF752_00965"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX8854140.1"
                     /translation="MDVHRFRLPDSAHTSQPWRIHELTRDFRLEDVWALPTPGGPHDF
                     PRLIDLIGSADPSRSSLPGRVLWAIRWRLGEVLGWDAPAAGVGSRVPTLRDRLPADLR
                     ETRPARAIDALPFSPLYLLDDEWAAEIANQTMHGVMHLGWVPDGTGSYRGQMAVLVKP
                     NGLLGMAYMAAIKPFRHLIVYPAMLGQLEREWRARVPDPVQA"
     gene            complement(1943..2767)
                     /locus_tag="VF752_00970"
     CDS             complement(1943..2767)
                     /locus_tag="VF752_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854141.1"
                     /translation="MRTLKRLCVAAAALALLALPASALASSKDRNHDRIPDRWEKRHH
                     LSLRVNQAKRDQDNDGLNNLGEFRSHTDPRDPDTDNDGVEDGDEDADRDGVDNENEIR
                     ERTNPEARDSNHNGRSDGREDADHDGLNNTGEDRTGNDPVDPDTDDDGVKDGKEVAGT
                     IASFNGTTLVINLAEGGQRTGTVNASTEIKCETEDELNDDESGSGHDSTRLSRDGSDD
                     GPNHDSGDDNSGPGSDAEHDGDEDNRCTTADLTTGTGVHEAELRLDGSFEEIELIK"
     gene            complement(2861..4105)
                     /locus_tag="VF752_00975"
     CDS             complement(2861..4105)
                     /locus_tag="VF752_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8854142.1"
                     /translation="MAGVLVTPRRLIEPARLAGSRMLLGTQSDERLVDLARAGYAAAF
                     EAIVERYRQPLLRYCLRFLPSGRAEDAVQQAFASAYRSLTTSDAHVELRPWLYRIAHN
                     AALNQLRDRGFHNEELSEEIDGVERPDQVAERRQRFSSVVAAVRDLPERQRDAILLRE
                     FEGRSYDEIAAALGVGRGAVRQLLHRARAGVRSAATALTPYGVAERLVGESGSRVAEA
                     AAGAGLGAVLTKASAAVLVTGAVVGGATQVPHHHHSGAQSGGGSTHESVVADAAAAPV
                     ASRHAAVARPEQTTREKARRRGHWRGTSKHGGHSGDRSGSGEHGENRRERTVSDDHRS
                     RGSTPDADEHTSGGGSSGSGSDDGLAAPDSSGQPQPVMTVEDTSEHDGSGTSGSSSGT
                     SGSGSSGSDSGSDGTHHVSDRR"
     gene            4188..4937
                     /locus_tag="VF752_00980"
     CDS             4188..4937
                     /locus_tag="VF752_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8854143.1"
                     /translation="MRLERLHPDGHADTTPTEAIGGLRFHERAPAERPYLALNMVSTA
                     DGKAAVEGRTRAISSDIDRDIFHQLRTQFDAVMVGAGTVRTERYGPITKTPELREKRR
                     HEGVREEALAVIVSGSLNLPEDVPLLQDRDSQVVILTQSEGELEGVKAQVSYLRSPGT
                     LDMRPLVEQLRSEYGVRSVLCEGGPTLNASLIPYGLVDELFLTLAPSIAGGANALTIV
                     AGMPLPELRGLQLLSVLRAEDELFLRYALRR"
     gene            4998..5831
                     /gene="ftsE"
                     /locus_tag="VF752_00985"
     CDS             4998..5831
                     /gene="ftsE"
                     /locus_tag="VF752_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX8854144.1"
                     /translation="MGATETHIRAREAARSKPAQPRGSGVPVVEFQDVTKVYDGGSLA
                     VDRVSMKIGGGEFVFLVGPTGCGKSTCIRLLMRELMPSSGEIFIAGRNIGEIPAKRVP
                     HLRRNIGVVFQDYKLLPNRTVYANVAYALEVVGESRDSIRRKVPDILRLVGLSTKLHA
                     YPDELSGGEQQRVSIARAFVNHPPLLLADEPTGNLDPETSIGIMQLIYRINRTGTTVV
                     VATHDKEMVDKMRRRVIELEEGRIVRDQLSGMYRPDESTREFAIRLRGELGVGDEGHP
                     N"
     gene            5831..6727
                     /gene="ftsX"
                     /locus_tag="VF752_00990"
     CDS             5831..6727
                     /gene="ftsX"
                     /locus_tag="VF752_00990"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX8854145.1"
                     /translation="MRLGFFVKEAFRALSRNAAPSLAAMLTILLTALVLGVFIPVVQA
                     TTSTANQVRSRVVVDVYVAQGTTPADKLALRQKLEQAPNVKRVEYISKEQALATEKKR
                     HPNSGWELLGSNPLPDSFRVTPVDPDGVQQIVDRLFPAGADGKRHGVVSSIGDVRNRE
                     QDTKKILSATGIVKLLAAGLAGLLVFASIALVANTIRLSIFARRREVEVMRLVGATNW
                     FIRWPFVIEGIFVGFMGGALAILLLWIVKDTFLDPLSDRFALIALPDTITFPALVAVL
                     LGSCVAVSAVGSGVTLRRFLKV"
     gene            6783..>7174
                     /locus_tag="VF752_00995"
     CDS             6783..>7174
                     /locus_tag="VF752_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854146.1"
                     /translation="MYRSRLLLAALLPALAILALGLWLGGHPDRLPQFLRDAFVDDQQ
                     AVHAEVIDKVRSDFYRPVSTEQLEQSSLRGIVQSLDDPYSRYLSPAEARRFSQDVRGE
                     FEGVGLSVRHHPRGLEVTTVFDGSPAKAA"
BASE COUNT         1014 a         2500 c         2475 g         1185 t
ORIGIN      
        1 ccgccgaggt gaaggcggag accgactact gctgcacctc ttcgaacgcg gtgcaggtgg
       61 tggagcacat ccgccgcgaa cacggcgacg acgtggagat cctcttcggc ccggacatgt
      121 tcctcggcgc ctacgtcgag aaggtgaccg gcaagcgcat gagcgtgtgg atgggcgagt
      181 gtcacgtgca cgcgggcatc cgcccgtccg acatcgcgca ggtgcgcgag gccaacccgg
      241 gagccgagtt cctgatccat ccggagtgcg gctgctcgac ttcggtcatg gagtacgtcg
      301 catctggcga cgtggacccg gaccacacgc acatgctctc cactggcggc atgctcgact
      361 tcgcgcgcta ctccgacgcc gacaccttca tcgtcgcgac cgagaccggc atgctccatc
      421 cgctcgagca ggagaacccc ggcaagacgt tcgtgcccgc gaatcgcgcg gccgtctgcc
      481 agttcatgaa gatgatcacg ctgccgaagc tgcgcgacgc gctgcgcgac ggcggcgagg
      541 cctacaccgt gaaggtcgac cccgagctcg ccgagcgggc gcggctgccg atcgagcgga
      601 tggtggcgat ctcgggcgcc tagggcgtcg gcagtccgag cacgcccgcg accgcgcggg
      661 cggccagtat ctcgtcgtgc ggggcctgtc cggcgagggc gatcacggtg ccttcgagcg
      721 ccccgatgta cgccagcgcg accgccctgg ccggtgggcc cggtggaatc gtcccggcct
      781 ttcggcccgc gttgatgaga cgcgcgcagc gctttaccag cgtctcggaa atccgctcga
      841 cctcgcgcgc caccggatga tcgcggctgc tgaactcgag ccgcagggcg atctcgaaac
      901 gggcaacgtc gcggcggcag aagacggcgt gagcgcgcgc caatgcgatc agcgcagagg
      961 ccgggtcgct cgcgcgctcg atcgccgtgc cgacctccgc gcgccaggtc tcgtccaccc
     1021 actcgagcgc ggccagcacc aactcgtcct tgtccttgaa ctggtgatag agcgcgccgc
     1081 gggtgtagcc ggcgtcccgt gccacctctt cgagcctcag cttgccgtag ccgtagcggg
     1141 acaggccccg agccgcggac tccagcagcg cgctgcgcgt gcgggcgctc cgttgggcct
     1201 gagttgcacg cggagcagta gtcattgagt tcgccttaca tacatgcgtg tatgtatgat
     1261 acggagaccg atggacgtcc accgtttcag gctcccggac agcgcgcaca ccagccagcc
     1321 ctggcggatc cacgagctca cccgcgactt caggctcgag gacgtgtggg cgctgccaac
     1381 ccccggtggg ccgcacgatt tccctcggct gatcgacctg atcggatcgg cggacccgtc
     1441 gcgatcgtca cttccgggcc gtgtgctgtg ggcgatccgg tggaggctcg gggaggtgct
     1501 cgggtgggac gcccccgcgg ccggcgtcgg atccagggtg ccgacactgc gcgaccgctt
     1561 gccggcggac ctacgtgaga cccggcccgc ccgcgccatc gatgcactgc cgttcagtcc
     1621 gctctacctg ctcgacgacg agtgggcggc cgagatcgcg aaccagacga tgcacggggt
     1681 catgcacctc ggctgggttc cggacggaac gggtagctac cgcggccaga tggccgtgct
     1741 cgtgaagccg aacggactcc tcggaatggc atatatggcc gcgatcaagc cgttccgcca
     1801 tctgatcgtc tacccggcga tgctgggaca gctcgagcgg gagtggcgcg cacgagtccc
     1861 cgatccggtt caagcctgaa acagcgcggg gcgcggcatc gtgccgcgcc ccgcgcttcc
     1921 tcgggtggtt cgcccgaatt cgctacttga tgagctcgat ctcctcgaag ctcccgtcca
     1981 agcgaagctc ggcctcgtga acgcccgtgc ccgtggtgag atccgccgtg gtgcagcggt
     2041 tgtcctcgtc cccgtcgtgc tcggcgtcgc tgcccgggcc gctgttgtcg tcgccgctgt
     2101 cgtggttggg gccgtcgtcg ctgccgtcgc gcgagagccg ggtcgagtcg tggcctgagc
     2161 ctgactcgtc gtcgttcagc tcatcctcgg tctcgcactt gatctcggtg gaggcgttca
     2221 cggtgccggt ccgctggccg ccctcggcga ggttgatcac cagggtggtc ccgttgaagg
     2281 acgcgatcgt gccggcgacc tccttgccgt ccttgacgcc gtcgtcgtcg gtgtccgggt
     2341 cgaccgggtc gttgcctgtc cggtcctcgc ccgtgttgtt gagtccgtcg tggtctgcgt
     2401 cctcgcggcc gtcgctgcgg ccgttgtggt tcgagtcgcg agcctcggga ttcgtgcgct
     2461 ccctgatctc gttctcgttg tccacgccat cgcgatcggc gtcctcgtcg ccgtcttcga
     2521 caccgtcgtt gtcggtgtcg ggatcgcgcg gatctgtgtg cgacctgaac tcgccgagat
     2581 tgttgagtcc gtcgttgtcc tggtcgcgct tcgcctggtt gaccctcagc gacaggtgat
     2641 gccgcttctc ccagcgatca gggatgcggt cgtgattgcg gtccttcgag ctcgcaagtg
     2701 cgctggccgg tagcgcgagc agggcgagcg ccgccgcggc cacgcagagt cgtttcagtg
     2761 tcctcatcga gttcctcctg aatgtgggcc acccgcggtg gctcttcgtg cctgaaacac
     2821 cggcacgcgg cggaactcac gctgggctgg cgcgaaagcg ctagcgcctg tccgagacgt
     2881 ggtgggtgcc gtcgctgccc gagtccgagc cgctcgagcc ggaaccgctc gtgcccgagc
     2941 tggagccgct ggtgcccgag ccgtcgtgct cgctcgtgtc ctcgacggtc atgaccggct
     3001 gaggctgacc actggaatct ggcgcagcga ggccgtcgtc ggagccgctc ccgctacttc
     3061 cgccgccgct cgtgtgctca tcagcatcgg gcgtggagcc gcgcgagcgg tggtcgtcgc
     3121 tcaccgtccg ttcgcggcga ttttccccgt gctcaccaga gcccgagcgg tcgccgctgt
     3181 gaccgccgtg cttcgacgtg ccgcgccagt ggccgcggcg acgggctttc tcgcgtgtcg
     3241 tctgttcggg acgcgccaca gccgcgtgac gtgacgcgac cggggcggcg gccgcgtctg
     3301 cgacgaccga ctcatgggtg gatccaccac cgctctgcgc gccggagtga tggtggtgcg
     3361 gcacctgcgt cgcgccgccc acgacggccc cggtgacgag caccgcggcg gaggccttcg
     3421 tcaacaccgc tccgagcccc gcgccggcgg ccgcctccgc cacgcgcgag ccgctctcgc
     3481 cgaccaggcg ctccgccact ccgtacggag tgagcgcggt cgcggcggag cggacgccgg
     3541 cgcgggcgcg atggaggagc tgacgcaccg caccgcggcc gacgccgagc gccgctgcga
     3601 tctcgtcgta gctccgcccc tcgaactcgc gcaacaggat cgcgtcgcgc tggcgctcgg
     3661 gcaggtcgcg caccgccgcg actaccgagc tgaagcgctg gcgccgctcg gccacctggt
     3721 cgggacgctc gacgccgtcg atctcctccg acagctcctc gttgtgaaaa ccgcggtcgc
     3781 gcagctggtt gagcgcggcg ttgtgcgcga tccggtagag ccatgggcgc aactccacgt
     3841 gggcgtcgct cgtggtgagc gagcggtagg cgctggcgaa cgcctgctgc accgcgtcct
     3901 cggcgcgccc ggagggcagg aagcgcaggc agtagcgcag cagcggctgc cggtagcgct
     3961 cgacgatcgc ctcgaacgca gccgcgtacc cggcgcgcgc gagatccacc agccgttcgt
     4021 cggactgcgt gcccaggagc atccggctgc ccgcgaggcg ggcgggctcg atgaggcggc
     4081 ggggcgtcac gagcacccct gccactcggg ggagattcgg cgcatcacaa gcatgagacg
     4141 tatcgacaat gcgaattcgt ccctgaaggc acagtaccct gccgcggatg cggctcgagc
     4201 gtctgcatcc cgacggccac gcggacacga cgccgaccga ggcgatcggc ggccttcgtt
     4261 tccacgagcg cgcgcccgcc gagcgcccgt acctggccct gaacatggtg tcgaccgcgg
     4321 acggcaaggc agcagtcgaa ggccggacgc gcgcgatctc gagcgatatc gaccgggaca
     4381 tcttccacca gctgcgcacg cagttcgacg cggtgatggt gggtgcaggc actgtgcgaa
     4441 ccgagcgcta cgggccgatc accaagactc ccgagctgcg ggagaagcgc cggcatgagg
     4501 gcgtgcgcga ggaagccctc gccgtgatcg tgagcggctc gttgaacctg cccgaggacg
     4561 tgccgctgtt gcaggaccgg gactcgcagg tcgtgatcct cacgcagtcg gagggcgagc
     4621 tcgagggagt gaaggcacag gtcagctacc tgcgctcgcc gggcacgctc gacatgcgcc
     4681 cgctggtgga gcagctgcgc tccgagtacg gcgtgcggag cgtgctctgc gagggcggtc
     4741 cgacgctcaa cgcctcgctc attccctacg gcctcgtgga cgagctgttc ctcacgctcg
     4801 cgccctcgat cgccggtggg gcgaacgccc tcacgatcgt cgccggcatg ccgctgccgg
     4861 agcttcgggg gctccagcta ctctctgtgc tgcgtgcgga ggacgagctg ttcctacgct
     4921 acgcgcttcg tcgctgagtg caggttccgt tgcacctgcc cccggcggcc cggaaactcg
     4981 atacaaccat ggaccgcatg ggggcgaccg agacgcacat ccgcgcgcgc gaggccgcgc
     5041 gcagcaagcc tgcgcagccg cgcggctcgg gcgtgcccgt cgtcgagttc caggacgtga
     5101 cgaaggtgta cgacggcggc tcgctcgccg tcgatcgcgt gtcgatgaag atcggcggcg
     5161 gcgagttcgt gtttctggtg ggcccgaccg gctgcggcaa gtccacgtgc atccgcctgc
     5221 tgatgcgcga gctcatgcct tcgtcgggcg agatcttcat cgccggtcgc aacatcggcg
     5281 agatcccggc caagcgcgtc ccgcacctgc gccgcaacat cggcgtggtc ttccaggact
     5341 acaagctgct gccgaaccgc acggtctacg cgaacgtggc ctacgcgctc gaggtggtcg
     5401 gcgagagccg cgactcgatc aggcgcaagg tgcccgacat cctgcggctg gtcgggctgt
     5461 caaccaagct tcacgcctac ccggacgagc tgtccggagg cgagcagcag cgcgtgtcga
     5521 tcgcgcgcgc tttcgtgaac cacccgccgc tgctgctggc cgacgagccg acgggcaacc
     5581 tcgatcccga gacctcgatc gggatcatgc aactgatcta ccgcataaac cgcaccggca
     5641 cgacggtcgt ggtcgccacg cacgacaagg agatggtcga caagatgcgg cggcgcgtga
     5701 ttgagctcga ggagggccgg atcgtgcgcg accagctgtc cggcatgtac cggccggacg
     5761 agtcgacccg cgagttcgcg atccgccttc gcggcgagct cggcgtgggc gacgagggcc
     5821 accccaactg atgcggctcg gcttcttcgt caaggaggcg ttcagggcgc tctcccgcaa
     5881 cgccgcgccg agcctggccg ccatgctcac gatcctgctg acggcgctcg tccttggcgt
     5941 gttcatcccg gtcgtgcagg ccacgacgag caccgcgaac caggtgcgca gccgcgtggt
     6001 ggtcgacgtg tacgtggctc agggcaccac gccggcggac aagctcgcgc tgcggcagaa
     6061 gctcgagcag gcgccgaacg tgaagcgggt cgagtacatc tcgaaggagc aggcgcttgc
     6121 caccgagaag aagcgccacc cgaactcggg ctgggagctt ctcggctcga acccgctgcc
     6181 ggactcattc cgcgtgacac cggtcgaccc cgacggggtt cagcagatcg tggaccggct
     6241 gtttccggcg ggcgctgacg gcaagcggca cggcgtcgtt tcctcgatcg gcgacgtccg
     6301 caaccgcgaa caggacacga agaagatcct ctcggcgacg ggcatcgtga agctgctcgc
     6361 cgcagggctg gccggcctgc tcgtgttcgc ctcgatcgct ctcgtggcca acacgatccg
     6421 gctctcgatc ttcgcccgcc gccgcgaggt cgaggtgatg cgcctcgtcg gcgccaccaa
     6481 ctggttcatc cgctggccct tcgtgatcga gggcatcttc gtgggcttca tgggcggcgc
     6541 gctcgcgatc ctgctgctgt ggatcgtcaa ggacacgttc ctcgacccgc tgtcggaccg
     6601 cttcgcgctc atcgccctgc cggacacgat caccttcccg gcgctcgtgg cagtgctgct
     6661 cggttcgtgc gtggcggtgt cggcggtcgg ctccggcgtc acgctccgcc gcttcctcaa
     6721 ggtctgaggg cctctaagta ccctctagtt gccgcgaggc gctgccttcg cggcctccct
     6781 ccatgtaccg ctcgagactc ctcctcgccg cgctgctccc cgcgctcgcg attctcgctc
     6841 ttgggctgtg gctgggcggc caccccgacc ggctgccgca gttcctgcgg gatgccttcg
     6901 tcgacgacca gcaggccgtc catgccgagg tgatagacaa ggtgcggagc gacttctaca
     6961 ggccggtcag cacggaacag ctcgagcaga gctcgctgcg cgggatcgtg cagtcgctcg
     7021 acgatcccta ctcgcgctac ctgtcgcccg ctgaggcgcg gcgcttcagc caggacgtga
     7081 ggggcgagtt cgagggcgtc ggcctctcgg tgcgtcacca cccgcgcggg ctcgaggtga
     7141 cgaccgtctt cgacggctcg ccggctaagg ccgc
//
LOCUS       DASVXS010000020        41895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_993773, whole genome shotgun sequence.
ACCESSION   DASVXS010000020 DASVXS010000000
VERSION     DASVXS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 41895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41895
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993773"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VF752_01000"
     CDS             complement(<1..506)
                     /locus_tag="VF752_01000"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX8854147.1"
                     /translation="MGTLAGASMRGDAALSRRGTRARALRSSLAIWACAFWPLIAAAV
                     LFRFAIANHFFGYDFQATIYDPGRAVIEGHSPYPSPTLAALIGRPTFVYPPLILWLDV
                     PIALLPLAAAQAVWAVLLVAAVAAGLWLLGVHDWRCYGLALGSVPVLDGLVMGNVTIL
                     FVLGLALA"
     gene            complement(510..1076)
                     /locus_tag="VF752_01005"
     CDS             complement(510..1076)
                     /locus_tag="VF752_01005"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX8854148.1"
                     /translation="MPSPETTAAQALPTPIGRASSTARLIASVGVGVAAAGAIARFGF
                     GAKGLIAACFASVLIVLAAVDLERRIIPNRIVLPATAVIMVAQIAHAPGRTPEWILAS
                     VGTALFLFLPSLIRKNAMGMGDVKLGLLLGAGLGKHVLTGVTLGFLAVWPVAIVLFVR
                     RGRDGVRDTAIPLGPFLAAGALVALFLD"
     gene            complement(1063..1473)
                     /locus_tag="VF752_01010"
     CDS             complement(1063..1473)
                     /locus_tag="VF752_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854149.1"
                     /translation="MTGVGAAARPVHCGRVLVATRDRGFAQAAAALLARHGYLVESTD
                     RPSRVPEMVGTVRPDVVIVDASGGCFFVPYVLAVVEAADSPAGVLQVGDADEPVARGA
                     FSLWKHGPPERLLAEVERVHAGVLAERGSVLAFS"
     gene            complement(1470..1877)
                     /locus_tag="VF752_01015"
     CDS             complement(1470..1877)
                     /locus_tag="VF752_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX8854150.1"
                     /translation="MSEPHTRTVLSDRAATVCERCQVANTAVPRMKGLLGRKRLAEEE
                     GLLLTPAPSIHTWFMRFPIDVVFLDRDLEVIDVVEALRPWRAAGRRGARAVLELAAGR
                     ARRCGLRIGDKLSLVAPGSIQTPVVFVRAGSAT"
     gene            2085..2285
                     /locus_tag="VF752_01020"
     CDS             2085..2285
                     /locus_tag="VF752_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8854151.1"
                     /translation="MDGRKRTRWEEVKMFDFIRTLREREEGQALVEYALILFLVSIAA
                     VAILTTVGSNIVTVLTSVANHL"
     gene            2469..2900
                     /locus_tag="VF752_01025"
     CDS             2469..2900
                     /locus_tag="VF752_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8854152.1"
                     /translation="MEFTLVLPIVLLLLLGMVDFGKAFNYWIDTTHLANEGARYAVVN
                     KNPSTTPGQSLQQYIQGEADTGELRNGGSSSLPTAAQVCIDFPNSTSNVGDPVRVTVR
                     ADYHLLPFLGDKLSISVPKALDSTATMRLEAPPSNYAAGCS"
     gene            2897..4870
                     /locus_tag="VF752_01030"
     CDS             2897..4870
                     /locus_tag="VF752_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX8854153.1"
                     /translation="MTARRGPSGERGGVLVMFALWLPILVLFITLVVDVANWFEHKRH
                     LQLQADAAALAGGGRFGAPCDDTAITDTATAYGGATYNAQVGGTAPANVHMEFNSQTY
                     FGQSSPVDSTVVTGSPCAALMLDVKMTETDLPWLFSVAKFVPFINAHARVSVRQIDNQ
                     QGALPVGVPDVNPKLAKVTFVDESDGTVLGSASMTRVGSDAQGNALWDNSGSPVPVTF
                     QKSGNPQRMDVGVRVALSGGNSTNCTDPLVNCYDAGSTNGGLDYIRGYSNAGSGTAAV
                     PIAREISFLPANGSCADPYFHVGTTACTVVLQAKVDFGTGTGNPTVSPTNADLQAFVD
                     GKNPAVNLTYNSTTHLWRSNPLTIQPQTGPVPIELRWTSNSSSCGKNGKCSGTFGNVQ
                     RSFSGLDDRSGPIQLLQVWEGLQQDTNAFEMCSSVQSSCTHNLVVHMAIKGSLKNAAD
                     VNDPIVSLRLVGNDSNSSRNQSLDCDPGYPNLKEELAHGCRPAYAKNTGQACPGSTTT
                     LWATAQPWNCVAIQTGTAINQVSQGMNERIQGSANSNTCVSPNNWSKFGTAQWDWSDP
                     RITPLFLVPYGSFGGSGSGTLPVIDFAYFYVTGYSGQGNGTDPCAGKGDDPAQPGYIV
                     GHFIKYVSSLGNSSGSTPCDPNAFGGCVVQLTE"
     gene            4882..5754
                     /gene="cpaB"
                     /locus_tag="VF752_01035"
     CDS             4882..5754
                     /gene="cpaB"
                     /locus_tag="VF752_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8854154.1"
                     /translation="MQVQTPPAQRPKKDRSKRDLRTLVSTRNGAMAVAAISALIAGVV
                     LLVFLNQYKDSLNGSDKPATVLVAQRLIEKGSSGDVIATQGLYQTTRIPKGQLKEGAL
                     SDPSVLRGKAAAHDILPGQQLTTADFGASTDPILQNLAGNQRAISVPVDNAHGMIGDI
                     HAGDRVDVIAGFNWQPNGMATATPVTKVLLQNVLVLRAPSGGARSSGPGGSDKSVVLE
                     VPDGKTGEIAFSSDNGKVWIVKRPKAGAKDTSQGIVSTETVLFGVKPIRTDSIQKTIS
                     AEIGKALKKAAKGK"
     gene            5754..7016
                     /locus_tag="VF752_01040"
     CDS             5754..7016
                     /locus_tag="VF752_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8854155.1"
                     /translation="MTEPIKILVVLDSGVEPELIQTVLAIEPEVQILAMADSLHQDWG
                     RTAEESADILVVACSGYSDRVLHFISAATAEHAERPLVVLSEGAANGFVRRVFEAGAD
                     DIVSLPDSPSAHVNGALAEEIVFTLQKALARKRGSMATLRDALGTLVCVLGPKGGIGK
                     TLTATNLAVALAEEGERTAIVDLDLQFGDVALAVGLAPERTIYDLATSSGALDAEKIE
                     AYMAQHDSGVYALLAPIRPDQASAVTVEFLREVYELLRGSYDYVVVDTPPGFTPEVIA
                     SIDASSHVCMVGTLDSLSLKNTKLGIETLDLMGYDQSRVSVVLNRADSRVGITREDVA
                     EIVGRPPDVLVPSHRDIARSVNQGAPIVLSGKRSEAARAFADLAGLYITNRSHALAPA
                     ANGAADANGRHNGKPRKRRSRRLLGRRG"
     gene            7016..8368
                     /locus_tag="VF752_01045"
     CDS             7016..8368
                     /locus_tag="VF752_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX8854156.1"
                     /translation="MELHERLYSQRPAEQPPQRDLFAELKNRIHVAVIGELGPQLFNT
                     DMDPAVLRTRVMSDIEGHLSQEVGLARPDRVRLSNEIADDILGHGPLEGPLADESVTE
                     VMINGPFDVWVERQGRLHETSIRFTDESHLRRIINKIVAQVGRRIDESSPMVDARLPD
                     GSRVNAVLPPLSLSGPLVTIRKFGNKRWDLNDLVRLGTLSRETVEFFDKCIRAELNIL
                     ISGGTGSGKTTLLNAMSTAIPDDGRIVTIEDAAELRLNQRHVLRLEARPKNIEGEGEI
                     PIRALVRNSLRMRPDRIIVGEVRGAEALDMLQAMNTGHDGSLCTVHANSPRDALSRIE
                     TMVLMAGFDLPVRAIRQQVASALDLIVHLERLHDGSRKVTAITEVQRMESDVITLQDI
                     YTFHVEGVTSGSAVIGKMVATGLRPTFQQKFEKHGIELPLDIFANGVGSPEMLRGAGL
                     "
     gene            8365..10236
                     /locus_tag="VF752_01050"
     CDS             8365..10236
                     /locus_tag="VF752_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8854157.1"
                     /translation="MRRLALAAACAQLALAVFAASVLAAAPTLTPAGGLGFPNRQFVL
                     SLPPGSGFDPSRIDVRENGRPVDALSVLPAGAAGSRQLGVVLVIDTSRSMKGDAIAGA
                     MAATRAFASHRNPQQQLGVVTFNGTASTLLPLTTDQAAIDKALAGTPALGPNTHIYDA
                     TEAAISLLKAAHISAGSVVVLSDGSDTGSGATNASVVADAKAAGIRIFAVGLRSGAFD
                     PATLSTLAKGGAGSYTEAGSKEELAKIYDELGAELSSQYIVRYRSSAAPHANVHVTAS
                     VTGTPLQATAAYNTPALPVNPPPPYHRSFWQSPGAVAAISVLCAGLLGLGLLTVLTSS
                     PAKRSLRARMRDFVSSPGEERGPRERVTSRLFVGAEKTLERRQWWATFKEELDVARID
                     IPPVRILLGTFTATVFLTWLLWFITGAAVVAVVGLATPLAVRFAIKRKLEHERRLFSD
                     ELADNLQVIASAMRAGQSFVGALSVAVQDAPEPARREFERVVADERLGVPLEEGLGVV
                     VRRMANHDLEQVQLVAALQRQTGGNMAEVLDRASDTIRERAELRRMIKTLTAQGRLSR
                     WVVSALPPSMVLILSAINPGYLDPLFKSTGGLTVLVISAGMVVGGSLVIKRIVDIEV"
     gene            10247..11161
                     /locus_tag="VF752_01055"
     CDS             10247..11161
                     /locus_tag="VF752_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8854158.1"
                     /translation="MTLILLTGLILVGFAVALTTRALTYSRTDAARRLAEIQSYGFGA
                     GAVEEAPPPASLGSALDHTAGRLGGWAARHLGSFREDDVRKQLTIAGIYSLTPITFLG
                     YRVLCTALFPALFVWCSSALGLPALLAVIGTLASFAVGWVAPMTYVRRRARQRFDKIE
                     RDLPELIDLLVLTVEAGVGFSGALQLAAQRLRGPLGDELRLTLQEQSMGLATNAALAN
                     MLSRSDTPSMRSFVRSVIQGESLGVSIGQILRNLATEMRKRRRAHAEERAQKAPIKIL
                     FPLVFLIFPPMFAVLLYPALSTFSNTFG"
     gene            11161..11832
                     /locus_tag="VF752_01060"
     CDS             11161..11832
                     /locus_tag="VF752_01060"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8854159.1"
                     /translation="MRRAAITSVFLLLAGPTAAAAGERVASAIDHPVLREWLVAGYWT
                     LKAAIVIAFATFVFTRGPARRPAREPVAFAACTVAIASAMALRSPSASDATAVVLAGE
                     VVSLVGCAWLFASAMALGRCFSVLPEARGLVTNGPFRLVRHPLYLGEMTIGAGLVIAS
                     PSLFNLTFASAFVGSQTVRMLLEERALTAEFPEYADYAASTPRIMPALSPLVGGPRTQ
                     REQRA"
     gene            11829..12881
                     /locus_tag="VF752_01065"
     CDS             11829..12881
                     /locus_tag="VF752_01065"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8854160.1"
                     /translation="MTAIPALGRRLGRVLSDTSETSEQPGAIRIRRAMATLFCFYGTV
                     LCVMAVTRGEVPSLSYVIIFMAAASLCVNRGGSFVRYWLPVLLGLYAYGVAGAYAQKL
                     KFGVHYLPQIDIERVISLGGTLPTTWLQEHLYHGHTDALAIAASITYLSHFFVPFLFA
                     FYLWWRQRKDAFTALMFGLLTVSILGEITFVLAPTAPPWMAAQEGLIPPVHHVIKQAL
                     FDMHFTKAAEFYGNSANYNTVAAMPSLHAAWPIVSYLVARNYGLPRWTRAVLAAQWLA
                     IVFAIVYTGEHYLADAVVAVPYALAASWLVGKALVRSRGHEKGGSPVTESGLALPGSV
                     PAPVTSPGALPEPVEG"
     gene            12885..13058
                     /locus_tag="VF752_01070"
     CDS             12885..13058
                     /locus_tag="VF752_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8854161.1"
                     /translation="MRIEIDSRWVAAGGAQDGQALVEYALITSIVSIVAIAILGTIGS
                     DLVTLITSVANAF"
     gene            13062..15113
                     /gene="ftsH"
                     /locus_tag="VF752_01075"
     CDS             13062..15113
                     /gene="ftsH"
                     /locus_tag="VF752_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8854162.1"
                     /translation="MVDAGPSTGAGARRGADAASPWRVEGSDSPPAATRKRGLRGWRA
                     PGGRRFWLLVALLLSVNIYVSAQITKGPERVGVPYTFFRDQVESGHVADVTTRGDMIQ
                     GEFKPPVAYPAGSKDNATRFQTTRPSFADDDLFGLLVKKNVTVNAKAIDQGRPLWLVL
                     ITSILPTLAIVALFLIVSRSMGGGLLRLGRSRAKLYDASAHRTNFEDVAGIDEAEEEL
                     AEIVSFLRDPGRYWELGAEMPRGVLLSGLPGTGKTLLARAVAGEADVPFYSLSASEFI
                     EMVVGVGASRVRDLFNQAKQNAPAIIFIDELDAIGRARGGSGGRAAGHDEREQTLNQI
                     LVEMDGFSGSEGVIVLAATNRPEILDPALLRPGRFDRRVTVNPPDAAGRERILAVHTR
                     RVPLSADVDLRQVAGGTSGMVGAELKNLVNEAALAAARRGSAEVSLIDFGEALEKIVL
                     GAERRITLSPSERERTAYHEAGHAVLGMLEPGADQVRRVSIVPRAQSLGVTFQSSETD
                     RHAYDADYLRGRIIGALGGRAAEELVYGKVTTGAESDLAQVTRIARQMVGRWGMSKAV
                     GLVSVVPGPGEESRVVADAGMAFSPATGELVDLEVRRIVAECYEAAVHRLRDNRPRLD
                     ALAAALLEHETLDEEAAYSAAGFEPGRVADSGHDGASAADARASAFSAAALVTPKLGQ
                     Q"
     gene            15276..15995
                     /locus_tag="VF752_01080"
     CDS             15276..15995
                     /locus_tag="VF752_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854163.1"
                     /translation="MKVLLADSDRLTLQGILRALEGVDDINVVGEARSGGEMLRLVER
                     VRPDMVLMDAHLPDIDGLECLDLMRRRHRDVKVVLLSASDGRERIQEALDRGATAYIV
                     KSVNPTDLPSALRQAFEGTVFSGFGRVKSEATAGSPAEGVLTERELAILRAVASGLSN
                     KAIGRELWVTEQTVKFHLTNIYRKLGVANRTAAAQYAHANGLLGASPGSEPARAGQIR
                     GGEAGFRTARPSMLDRVLPAR"
     gene            16159..16653
                     /locus_tag="VF752_01085"
     CDS             16159..16653
                     /locus_tag="VF752_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8854164.1"
                     /translation="MSVIDELVDANTHFADALGGVESPALPPRRRVAIVTCMDARIVP
                     ARIFGLEEGDAHVIRNAGGLAREALRSLVISQRLLGTSAIAVVHHTDCGMLTFQNEDV
                     REKVQQDLGADASDIDFMPFAELDQSVRDDVAFLRDSPLIAPDTEIRGFVYDVSSRRV
                     NELA"
     gene            complement(16661..17473)
                     /locus_tag="VF752_01090"
     CDS             complement(16661..17473)
                     /locus_tag="VF752_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854165.1"
                     /translation="MLERQRAGPNGASDLSIDVWRSRGRELNLRMPFPGLEQNPDDGG
                     RRRWPMLDSLGRTLALLEQDADGLVTTDPETGREIHRERTGRLSLQGRGAMADGALES
                     SQALVAFDARDRSELAPGASIVPFRIRAFVSRDALPRRVGFSRVRAKVDTARTDRGEC
                     DLTPLSRVPLQDPGFRSDERFVGEDGRARTYATYSAKRPYGGAMYICVNTTGVHGGGI
                     VRAVAREGDEVDVLDELPEPDSNAASGTPLACWRFLRLAGTSICGWVPARAG"
     gene            17504..18946
                     /locus_tag="VF752_01095"
     CDS             17504..18946
                     /locus_tag="VF752_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8854166.1"
                     /translation="MSDVQASLEEYRSVRQTLEKAVLPLATSVDGRRFSFQTSLHGLD
                     LEAGGYVKLDGAASSRLGQVLSLNLSSQDGSAPGLPTVRIRVGTGDGVVLAGDGHPFH
                     DALVSPARPEDVATWLDRIRPERAAIEVGELALAPGVPFWLDAGGFGRHTFICGQSGS
                     GKTYSLGVLLERLLMGTRVRVVVLDPNSDYVRLGEMRENADVDTAERFATVAPDVAVR
                     SGAGGPDGIRVRFRELSPAQQGALLRLDPLADREEYAELLSLVEDEGIRSVAELEGVE
                     SDALRLRARNLGVDRWAIWPGREGGSLAAELEDPNGARCIVVDLGSLGSREEQAITAG
                     TVLERLWRRRARREPIVIVIDEAHNICPAEPADAITAIATEDAIRIAGEGRKFGLYLI
                     VSTQRPQKVHENVLSQCDNLVLMRMNSIADLAHVGSVFSFVPPGLLDRATTFGQGEAL
                     VAGKIASHPAVIRFASRITHEGGADVAGWA"
     gene            complement(18949..20154)
                     /locus_tag="VF752_01100"
     CDS             complement(18949..20154)
                     /locus_tag="VF752_01100"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX8854167.1"
                     /translation="MDAGVTRRVLVQRGLLAAGGAAGGLSLSRSGAIGAPRPGPAAGT
                     WLPGDLHTHSTYSHDVYGGPMDDNTGPDEAYTLGLPVAGKYAEARERGLRFLAVSDHN
                     DVRSVPELGSAAGGLIAIPAYEHSIKGHAQMLGATRLHDAGDQAADVINAMADELRGA
                     GGVFQANHPAYRLPLDAEFHSCGSGGCADCQSINWTYGFDVRPDTIEVWNPSVARSDV
                     SETYWECWLDRGERIAGTGGSDSHWLSLHAVAGPGQPTTWVYAPEASVAGVLQGLRAG
                     RTSVSGQPPSLGGAQLLLDAPGEGIIGDAVAPGTAMRVTAPGLTVPAVVRVRANGQQI
                     LERELEPGGEVRFAAPRETGWVRAILLGREALPVDTTTDVGNATPQRDGMPLLALTSP
                     MYLSRRRRG"
     gene            20270..20785
                     /locus_tag="VF752_01105"
     CDS             20270..20785
                     /locus_tag="VF752_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8854168.1"
                     /translation="MSIGTHDHVDRVLDEWSRERPELDTTAMGVIGRLMLVARLADGR
                     LSAPLRERGLDRGWFDLLAALRRAGRPYELNPKALMESVMLSSGGMTKRLDNLERAGL
                     VERRPDPADRRGTLVRLTPSGRRAVDRAVELHIANEDALLDSLDDDELGALDDLLRKL
                     VAALERKEAAA"
     gene            20782..21702
                     /locus_tag="VF752_01110"
     CDS             20782..21702
                     /locus_tag="VF752_01110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8854169.1"
                     /translation="MTPTTMRAVLLREPGGPEHLGCEQVATPAPGPGEVLVRVHAAAI
                     TRGELEWPVDRLPAIPSYEVSGVVAALGPGVDDVAAGDEVYGMTAFDRDGVAADYAAI
                     PSELLAPKPRGLSHAEAAAVPLAALTAWQGLFDHGTLAPSQRVVIHGATGGVGQFATP
                     LAKRHGAHVIATASADAAERARQLGADEVVDYTVGQWATGIEPVHLVFDTAGGERLAA
                     SPRVVRSGGRLVSVAEEPPEATGISSVYFVVEASRDQLTEIARLLDEGFPAPAVDSTF
                     SLEEAKSAFERSMAADKRGKVALTSHAEKA"
     gene            complement(21721..22230)
                     /locus_tag="VF752_01115"
     CDS             complement(21721..22230)
                     /locus_tag="VF752_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8854170.1"
                     /translation="MNPEQTKSVSPQRREEIRQRQRAANEVRNALRELRIQLALLNYR
                     VGLQVGLRDVDLDCLDILDTSGPLSPSELARRAGLHPATLTGILDRLERGGWIVRERD
                     PNDRRAVVVRVVHDRYGELLGLYSAMSRSMNKILSDYSDEQLEVIADFLRHTVEAGRN
                     ATEGLASED"
     gene            22290..23765
                     /locus_tag="VF752_01120"
     CDS             22290..23765
                     /locus_tag="VF752_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854171.1"
                     /translation="MSTPASTPSFLATRRGKLTLVLLAAIAFLDFVDASITNIALPSI
                     RHDLGVSVQDLQWVPSGYLLTYGGFMLLGGRAADLLGRRAVLVAGMSVIGISSLIGGI
                     AGSPGVLIASRLAQGLGAAMTLPAALSILTTTFKEGSDRTKALGIWGAAGALASAAGV
                     MLGGLLTEGPGWRWVFFVNPIVVVLLLLATFRLISGERPNAQRLRNFDIPGAVLATGG
                     MLLLVYTLVKAPDIGWGTGRTIAGLAGALALLATFVVNELRSRNPIAPLSIFRINGLG
                     FADATQLIAFAGFLAMFFFLTLYMQNVLGLSAIQTGAAYLPLTFVGGIAAGASSQLVS
                     RVGTRPVIVAGAVISAAGLYWLSRIPADGSYFGSVLPGMLVLSLGFGPVFVGVTTAAN
                     AGVPADKAGLAAALLNASQQLGGALGLAIFSALATSRTHDLLAAHTPPAEALTSGFQR
                     ALLAGSIFILASAVIALRATNTRGETPDAAAHRPVPVPEGA"
     gene            complement(23987..25501)
                     /locus_tag="VF752_01125"
     CDS             complement(23987..25501)
                     /locus_tag="VF752_01125"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX8854172.1"
                     /translation="MSTTTVRTIQHRVGGQETSGSSTRTSPVWDPATGEQQAEVLLAE
                     REDVDAAVAAARAAFESWRDVSVTRRAKIMFAFRELVERHVDDLARVVASEHGKIVED
                     AKGEVIRGLEVVEYACGIPQLLKGEFSEQISTDVDSFSFRQPLGVCAGITPFNFPVMV
                     PMWMHPMAIASGNTFVLKPSERDPSASNLVAELYAEAGLPDGVFNVMHGDKVAVDALL
                     DNQDVAAISFVGSTPVAKYIHARATENRKRVQALGGAKNHAIVLPDANLEFAANQLTA
                     AGYGSAGQRCMAISVGVAVGPAGDELVQQLSERARSIKVGPGLDPESEMGPVVTAQAR
                     DRIAGYIQQGADDGADVVVDGRELETGSGGFYLGPTLIDRVTPDMSVYRDEIFGPVLS
                     MVRAEDLDEAIALINANSFANGAAIFTSSGRAARRFQRDVQVGMVGVNVPIPVPMAFH
                     SFGGWKQSLFGDTHIHGPEGVRFYTRGKVVTSRWPEEVPGAAQERGHLHFPTAE"
     gene            complement(25645..27279)
                     /locus_tag="VF752_01130"
     CDS             complement(25645..27279)
                     /locus_tag="VF752_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8854173.1"
                     /translation="MATEEVARTTTPQTGLFVRNATGLVREVKPWQAMAINFITGAPP
                     FVIGIALFGALSGFPGGNFLVATLLTIPLALSVVYTFGLLTAAMPRSGGDYVLVSRIL
                     HPAAGVVSSVCISLSSFLSIAFEALAMVTLGLAPALLIVGLIGDSGTLVKWSNSVATE
                     HGWQFGLGVVGILAAGIAVGAGWTWAKRFMFGLLGFSLLGLLVSALIALFTSKSSFIG
                     HFNSFAQPITGNPDTYHSVLSTAQKNGVDLSTGFSFSQTIPMVAVVAGISIYAYWSTF
                     FAGELRQGNTMKTANRMGAASALILLSVIVFVAIFFNSFGRQFMTAAFGGGLPDKLGT
                     SGAYFVLTSAQVNGIVFAVFLCLSFVLFWPVIMAETALQPPRTLFAWSFDGIAPKAVT
                     QVSRRGVPLTATIITIVLAIAAYAWAIFISNSFFQVVVYATLIQLITHTLVAISAISF
                     PYRKPELYRASVSAKTVAGIPLVVITGIGAILTTVFLYFCYFHYSFFGLKDKSQLWIW
                     LGGAIAFGLAWYYGARAIRRNEGVNVDRVYAEIPPE"
     gene            complement(27297..28286)
                     /locus_tag="VF752_01135"
     CDS             complement(27297..28286)
                     /locus_tag="VF752_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854174.1"
                     /translation="MRRLFSRRGHAPDEDALELYYASDIHGSDRCWRKFLGAARFYGL
                     SDVIMGGDLVGKAVVPVEHRPDGTYMCRFLGDERVAVDDEQLAEIVDAIRFNGMYPWI
                     APPEELARYAGNGAGQDELFDRVVREDLDRWIALAAERLPENGTHAFVMAGNDDPWYV
                     DEMLASADAIEFCDDRVVRVGDHEMISCSYANPTPWDSPRELDEEALYRRLKSLAEQV
                     EAPERAIFNLHVPPYDSGLDKATELKPDLTPRFSGGQPVEIPVGSHAVRQIIEEYQPL
                     LGLHGHIHESRGEAWIGRTLALNSGSEYNTGRLHGVIVKLGRERVISHQFVVG"
     gene            complement(28288..29058)
                     /locus_tag="VF752_01140"
     CDS             complement(28288..29058)
                     /locus_tag="VF752_01140"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8854175.1"
                     /translation="MSDDAPGAELAPRADIVAEARRVLDAANARDVPLRLIGGLAIAL
                     VAGDNRLLPRDYKDIDFVARRGMGPEVVALLDSLGYEGNQQFNALNGHRRLLYYDNAH
                     GRQLDVFLGSFQMSHQLPIQERLLVARDTLPLADLLLTKLQVFALNEKDQRDVLTLLF
                     HRPLSDDDADGAINCDYVASLCSRDWGLWRTCTLNLERIRSAVARYDLAPEQQDVIVS
                     RIDELRRRIDDAPKSTRWKLRARVGERVRWYDEPEEVT"
     gene            complement(29371..30420)
                     /locus_tag="VF752_01145"
     CDS             complement(29371..30420)
                     /locus_tag="VF752_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8854176.1"
                     /translation="MKATQSRAPGRNPTIFDVAALAGVSKSTVSNVVRGNDGVSAATR
                     ARVERAIEILGYRPNVLARQFVSQRTTILGVLVGDLDNPFYGDLAKRLERLAFRSGYT
                     TMFCNIEGAGDEEADFAASRVEALLEQRPAGVVFLAFFGRSPDIAAALGGVPVVFAGL
                     REDWGDSVAVSNSAGGKLATEHLLDLGHRRIAYLTTHSLERRAHVARHRGYQAAMRGA
                     GVDPAPPIHWGPGSEEATVKGADLSLLDQLRDTTAVFCSNDIGAIAVLEYADRHGLRV
                     PEDLSVVGFDDVHLASLARISLTTIRQPFDDIARIAVDTLLGRLSGTLEGPPRHTQLA
                     PALVVRSSTAAPAAT"
     gene            30600..31739
                     /locus_tag="VF752_01150"
     CDS             30600..31739
                     /locus_tag="VF752_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8854177.1"
                     /translation="MERRKRVGVIGCGLIAQVMHLPYLTELSYRFEIAALCDVSEEVL
                     EGCAERYGVTRTFTRWEELLEEPLDAVMVLTAGNHAPMAIAAARKGLHIFAEKPMAIS
                     ASEGAAMLGAADEAGVRLMVGTMKRYDPAYERFVQLAPEIDDLRLVRVTTLESPLEPY
                     VAHYPMVRPSSPLPPELIAQWNAEEAPIIDAAIGADASEDDRYCYRNILLDNLVHEFN
                     MLRGLLGEPSAVTYANLAPQCVAVNMRFGDAECHLSWVDLPGIARYSQQVALYGPDRR
                     LVLTLPSPFLRSAPSKLTTEGGEPGTSHGWDRDEIVSYDEAFKRELVEFHEAMLEGRD
                     ARTSGVDGLRDLMLCEAIARVHRTREPVTNPTELPAAVAAEVGGS"
     gene            31739..32695
                     /locus_tag="VF752_01155"
     CDS             31739..32695
                     /locus_tag="VF752_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX8854178.1"
                     /translation="MTQEIGERRLQEPATSEIVVANAPVSYGAFEITVGVYDNVPQPL
                     ELLDAVSSAGYEGVDLGPLGYLGTGSELAERLGSRGLSLAGGYFELPFSEPDRLKEAL
                     PELDALLDVFAAVPDGRVKARPTLADAGSPERAAYPGRAATDRSVGLDEKGWNNFADG
                     LARAVERCRERGYEPTFHHHTATYIEAQWEIERLLELSDVGVTLDTGHLLLGRGDPVK
                     AVHDWGERINHVHLKDAHRDVLEAIVADRAPVMEIWTRKAFCALGDGDVAIDDVLSGL
                     REIGYSGWLVVEQDTIPAAGESVDKAAGEQARNREYLRERGL"
     gene            32700..33641
                     /locus_tag="VF752_01160"
     CDS             32700..33641
                     /locus_tag="VF752_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8854179.1"
                     /translation="MAEAVRIAMVGAGRMGRVHLDALTRCRHAEAVAVVDPVPAARDA
                     AGAAGLAGYATLDELLDAGGFDAVLVAAPSDRHRRIVTTLARAGLPVLCEKPCGTSAD
                     EAAETATAVEAAGTPLQIGYWRRFVPELAALRERISEGELGEITLVACHQWDRELPHP
                     AFRASSGGITVDLGVHEYDQVRWLLGQEFETVAAVPAGSSDADAGPTDPDSAAVLARL
                     SGGTAASITLGRHFGQEDSCWFEAFGRESYERIPFMWGDAGQEAFVTALTAQADAFAG
                     LVRGEPLRGASAADAVAALETAGRVAEALERAPAEVG"
     gene            33645..34598
                     /gene="iolC"
                     /locus_tag="VF752_01165"
     CDS             33645..34598
                     /gene="iolC"
                     /locus_tag="VF752_01165"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEX8854180.1"
                     /translation="MAPFDVITTGRVGVDLYPQQSNVGLADVETFAKYLGGSATNVAV
                     QAARLGSRSAVVTKVGDDPFGPFVRRALEEFGVDPRWVGTDPSLRTPIVFCEVHPPDN
                     FPLLFYREPKAPDMNLTPEDFDAQAVRDATVFWTTGTGLSDEPSRSTTLAMLEERSGD
                     GARGITIHDLDHRPMFWEDEADATRWAREALRHATVAVGNQDEVAVAVGTRDPDEAAQ
                     ALLDLGVETAIVKRGPEGVLARTREERVDLPPVRLEVVNGLGAGDAFGGTLAHGLGHG
                     WELERTIRAANAAGAIAASRLACADDFPTLAEIDEVLAAHA"
     gene            34591..35400
                     /gene="iolB"
                     /locus_tag="VF752_01170"
     CDS             34591..35400
                     /gene="iolB"
                     /locus_tag="VF752_01170"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX8854181.1"
                     /translation="MPELLIHPGEPRPGEPVVRVTPESAGWDYTGFEVHRLADGEERS
                     FDADGRELCAVMLGGRADVGAGDRSFEGVGDRANVFDGLPAAVHAPAGHALSVRAVGG
                     EAEVALCWSPAEGTDALEPALIEPGDIQVEIRGSGSMERRVHPILMADRQAERLLVVE
                     VITPGGNYSSYPPHKHDRDDPPRETLLEEVYYHRIDPAQGFGMQRVYTDDRSLDETIT
                     FSDGDVVLVPRGYHTVSAPPGYDVYYLNVMVGPRREWHFVNDPDHEWLFGR"
     gene            35397..36563
                     /locus_tag="VF752_01175"
     CDS             35397..36563
                     /locus_tag="VF752_01175"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8854182.1"
                     /translation="MNRPAGTVRAAVMKEPGRLELERFPLPDPEPGAVLLKMSYSGIC
                     GTDKHTFRGETLQYAGTPHERRLEYPLICGHENVGVVAAVGGSEPVLDSEGVPLRDGD
                     RVVPAANVACGSCWFCRNDQPYYMCEHLDDYGNSLNAKRPPHLFGGWAEYMYILPGTP
                     LFRVPDELPDEIAVLTEVMAVTHGFELARRLYNTNFGESVAVIGVGPLGMCHLIKAKL
                     LGCGKLIAIDRLPSRVEMAGRFGATLGLDASQLDDEELIARVREHTGGRGADIVLDCS
                     GVPETFVTSLHMARVGGTVVESGAFVDMGPVAINPNADICTKNVNVLGVGGETAESYQ
                     PAMELLARNMERLPLHELVTHRMALEDAQAALEIAQNDGAMKVVLSGAVAPAGD"
     gene            complement(36560..37591)
                     /locus_tag="VF752_01180"
     CDS             complement(36560..37591)
                     /locus_tag="VF752_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8854183.1"
                     /translation="MNHSDGTQFRRPTINDVAALSGVSKSTVSNVMRGTGSVSAGTRA
                     RVLEAVAALGYRPNATARNLVLQRTNLIGVVVGDLANAFDAELVKRIEQQASGRGYTT
                     LVCNTGGHAEREGSRIEALLEQRVDGIALLDFTGDRAVLSQLLGERVPVVMVSCWSDY
                     TDCVAVDDMVGVELAVEHLVALGHKRIAHVVDPLMEVTTRRARAEAFERAMLRGGVSP
                     RSEWTLVFDPDDEDGERELMSLFSAAERPTAVAAANDYTAIRLMELLERHGIQVPADV
                     SLVGFDGIEIAGLSRIALTTVTQPRDELAARAVELLLHRIEGDDDAPPARHRMNPALV
                     VRHSTAPPA"
     gene            complement(37588..38628)
                     /locus_tag="VF752_01185"
     CDS             complement(37588..38628)
                     /locus_tag="VF752_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8854184.1"
                     /translation="MKPGRPRWGSQRPTIVDVANLSGVSKSTVSNVIRGSGRVSETTR
                     RRVLESIEALGYRPNALARDLVRRRANTIGVLVGDLSNTFYAELVKLMELEASESAYT
                     TMVCNTDGDLALERERVETLLERAVLGVVLLQFSGDRTVLDELRAAQMPVVVVSCADE
                     RADCVAVDDAAGMAAAVEHLYALGHRSIAHVSGHLVEETTRATRHAGYERAMRAHGLQ
                     PRLLSADDWEDESPSALRSLRELVDGGGGLTAFAAGNDVMAVRLIDALEAAGLRVPED
                     VSVVGFDGIELGAHERIGLTTVSQPRTELTTRGLELLLDRIHAGGVSAPPRRVTLVPE
                     LIVRHSTARART"
     gene            38833..39798
                     /locus_tag="VF752_01190"
     CDS             38833..39798
                     /locus_tag="VF752_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX8854185.1"
                     /translation="MRLALLTDALEDRSQEDAFAWCAERGIAGVELGVGGYSPAPHLD
                     LAALLASEAERERLTETLEEHGLELVALNASGNPLHPDPAIARDHDRALRGALELAAA
                     LDVRRIVAMSGCPGGPGSGGWPVFAGGAWLPDMEGLWDHQWENAIAPYWRELSAWAHE
                     AAAGVDICLELHPGTSIYNAASFDLLSEATGDNVKVNLDPSHFWWQGIDPVTTVRALG
                     DRIRFVHGKDTIVYPDRVALQGVLDFRWPADADTMPWHFCAVGHGRPLDEWRELFDAL
                     SEAGYDGPVSIEQEDPTLSPEQGIEASLEGLRAVMEATVPGQPER"
     gene            39816..40922
                     /locus_tag="VF752_01195"
     CDS             39816..40922
                     /locus_tag="VF752_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854186.1"
                     /translation="MDVQYKGVSKTFGSTEALAPLDLTVPDGKFLAMLGPSGCGKTTA
                     LRLLAGLEQPTTGSIFIGDRDVTRLEPRHRDVAMVFQSYALYPHKSVADNIAYPLRLR
                     RGSSAERDERVRNVAELLGISELLDRAPRQLSGGQRQRVALARAIIRHPQAFLMDEPL
                     SNLDAQLRLQMRVEIKRLQRELGVTTLYVTHDQIEAMTMADLIAVMRDGHLQQLATPA
                     ELYAHPTNLFVARFCGSPPMNILDGEVTDGAFRHAGGSISLRDAPVRGPVKLGFRPEH
                     AAFAEPGTADALEGEIYVVEPLGNETLVTMRVDGQLLNVRTSAGFSRPVGERCAVRPV
                     PEQVHLFDTESGGALAQTGARSAESAGQPATAGF"
     gene            40946..>41895
                     /locus_tag="VF752_01200"
     CDS             40946..>41895
                     /locus_tag="VF752_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8854187.1"
                     /translation="MSDQERDAARPEGSRITRRKVIERGAVGAAGIFGASFIAACGGS
                     SSSSSTATNASKGASGTITIGSFQDNAMAPFRDTFIKRFTKETGINVKYNETSYDAWY
                     QNAKNDGLNKTGAYDIYVMDDNWVPEFAAGNIIKNLDEMGFKVNQDILPKGLNQGYWP
                     PKEGARLKDFANDDPALYAIVIIDDVELLYYNTDHFKDAPKTWDEVFSGMKSKANPPK
                     LYGWSARGVKGNPIMMTYLPLLNSYGGEFVKDDWSPGFAGPEGVGALERLMTFIPYMP
                     SGVAEFDTDQETQSMLQGKCMALTEYTGLVHRVDDKKSSQV"
BASE COUNT         6632 a        14458 c        14390 g         6415 t
ORIGIN      
        1 caagcgagcg ccagcccgag cacgaacagg atcgtcacgt tccccatgac caggccgtcg
       61 agcaccggaa cgctgccgag cgcgaggccg tagcagcgcc agtcgtggac gccgagcagc
      121 cataggcccg ccgcgaccgc cgcgacgagc agcacggccc agaccgcctg cgccgcggca
      181 aggggcagaa gcgcgatcgg cacgtcgagc cataggatca gcggcgggta cacgaaggtg
      241 ggtcgtccga tcagcgccgc gagcgtgggc gaggggtacg gcgaatggcc ctcgatcacc
      301 gcccggccgg ggtcgtagat cgtcgcctgg aagtcgtatc cgaagaagtg attggcgatc
      361 gcgaagcgga atagaaccgc ggctgcgatg agcggccaga acgcgcacgc ccagatcgcc
      421 agagatgaac gaagggcgcg ggcgcgggtg cccctgcggg acagggcggc atcgccgcgc
      481 atcgacgcac ccgccagcgt gcccacggct cagtccagga agagcgcgac gagcgccccc
      541 gcggcgagga acggtccgag cggaatcgcg gtgtcgcgca ccccgtccct tccccgacgc
      601 acgaacagga cgatggcgac cggccatacc gcgaggaacc cgagcgtcac tcccgtgagc
      661 acgtgcttgc cgaggccggc gccgagaagc agcccaagct tcacgtctcc catgcccatc
      721 gcgttcttgc ggatgaggct cgggaggaag aggaacagcg cggtgcccac gctcgccagg
      781 atccactccg gagtccggcc cggcgcgtgc gcaatctgcg cgaccatgat caccgcggtc
      841 gccggcagca cgatgcggtt ggggatgatg cgtcgctcga ggtcgactgc cgcgagcacg
      901 atcaagacgc tggcgaagca cgcggcgatc agccccttgg cgccgaagcc gaagcgcgcg
      961 atcgcacccg cggcggccac gccaacgccc acgctcgcga tcagccgcgc ggtggagctc
     1021 gcccgtccga tcggcgtcgg gagcgcctgt gccgcggtgg tctcaggaga aggcaaggac
     1081 cgagcccctc tccgcgagaa cgcctgcatg gacacgctcc acctcggcga gcaggcgctc
     1141 gggcggcccg tgcttccaga gcgagaacgc cccgcgggca acgggctcgt ccgcgtcccc
     1201 cacctgcagt acgcctgcgg ggctgtcggc cgcctcgacg accgccagca cgtacgggac
     1261 gaagaaacac ccgccgctcg cgtccacgat caccacgtcg ggccgcacgg tgccgaccat
     1321 ttccggcacg cggctagggc ggtcagtgga ctccacgagg tagccgtggc gcgcgagcag
     1381 cgcggcggcc gcctgcgcga agccccggtc gcgagttgcc acgagcacgc gcccgcaatg
     1441 caccggcctg gccgctgcgc cgacgccggt cacgtggcgc tcccagcgcg cacgaacacc
     1501 accggtgtct gaatcgagcc gggggcgacg aggctcagct tgtcgccgat ccggaggccg
     1561 caccgtcgcg cccgcccagc tgcgagctcg agcactgccc gcgcgcctcg ccgtcccgcg
     1621 gcgcgccagg gccgcagcgc ctctacgaca tcgatcacct cgaggtcgcg gtcgagaaag
     1681 accacgtcga tcgggaagcg catgaaccac gtgtggatcg agggagcggg cgtgagcaat
     1741 aggccctctt cctccgcgag gcgcttccgc ccaagcagtc ccttcatgcg ggggacggcg
     1801 gtgttcgcga cctggcaacg ctcgcaaacc gtcgcggctc gatcgctcag caccgtgcgc
     1861 gtgtgaggct cactcatgcc gccccactat cgccttgatc gcataggccg gcaccacctg
     1921 caggtctagc ttcgaactgg accttcgggt ggtgcgtgtg tgcacaggcc gggcttacgg
     1981 tcgtccacgg cgaggcgaat ccgccatctc cagatgatca ctcgaaacga acatcaggag
     2041 cgagttccgg cgtcgggcct ctgccagtcg attacacggc gcccatggat gggcgcaagc
     2101 ggactcgctg ggaggaggtg aaaatgttcg acttcatccg cacgctccgt gagcgcgagg
     2161 agggccaggc gctcgtcgag tacgccctga ttctcttcct cgtctccatc gcagccgtgg
     2221 ccatcctgac cacggtgggc agcaacatcg tgaccgtgct cacgtcggtt gcgaaccacc
     2281 tctaggccag cggccggaag caacgatcca atcgccgtcc cgccggcgcg ggccttacgg
     2341 ccggatgaat gtccggcggg acggcgacat ggattctccc aaaaggatct cgtacgccgt
     2401 tgattacgtc cactccattt tcacgttttg tcagaagtgc acatgcagac cagcgcggtg
     2461 cggcgctcgt ggagttcacc ttggtgcttc cgatcgtgct gctgctgcta ctcggcatgg
     2521 tcgacttcgg caaggcgttc aactactgga tcgacacgac ccatctcgcg aacgaggggg
     2581 cgcgctacgc agtggtcaac aagaacccgt cgaccacccc aggccagtcg ctccagcagt
     2641 acatccaggg cgaggcggac accggcgaac tccggaacgg agggtcgagc tcactgccga
     2701 cggcagcgca agtctgcatc gattttccga acagcacttc gaacgtcggt gatcccgtgc
     2761 gcgtgaccgt cagggccgac taccaccttc tgccgttcct cggcgacaag ctgagcatct
     2821 ccgtaccaaa ggcgctcgac tcgaccgcca cgatgcgctt ggaggcgccg ccctcgaact
     2881 atgccgccgg ctgctcatga cagcgcgcag aggaccatcg ggcgagcgcg gcggcgttct
     2941 cgtgatgttc gctctctggc ttccgatcct cgtcctcttc atcacgctcg tggttgacgt
     3001 ggcgaactgg ttcgaacaca agcgccacct ccagctccaa gcggatgcgg ctgctctggc
     3061 gggcggtggg cggtttggcg cgccgtgcga tgacaccgcc atcactgaca cggctaccgc
     3121 ctatggcggc gcaacctaca acgctcaggt gggcggcacg gcgcccgcga atgtgcacat
     3181 ggagttcaac tcgcagacgt acttcgggca atcgtcgccg gtggactcca ccgtcgtgac
     3241 gggctcgccg tgcgcggcgc tgatgctcga cgtgaagatg accgagaccg acctgccgtg
     3301 gctcttctcg gtcgcgaagt tcgtgccgtt catcaacgcc cacgcccgtg tgtcggtgcg
     3361 gcagatcgac aaccagcagg gcgcgcttcc ggtcggcgtt ccggatgtga atccgaagct
     3421 cgcgaaggtc accttcgtgg acgagagcga tggaaccgtg ctcggcagcg cgtcgatgac
     3481 gagggtcggc agcgacgccc agggcaacgc gctctgggac aacagcggct cgccggtgcc
     3541 tgtcactttc cagaagtccg gcaatccgca gcgcatggac gtgggtgtcc gcgtcgcgtt
     3601 gagcggcggc aacagcacca actgcacgga cccgctggtc aattgctacg acgccggctc
     3661 cacgaacggc gggctggact acatccgtgg gtactcgaac gcaggctcgg gcaccgcagc
     3721 cgtgccgatc gcgcgcgaga tctcgttcct gcccgccaac ggcagctgcg ccgatccgta
     3781 cttccacgtc gggacgacgg cctgcacggt cgtgctccag gccaaggtcg acttcggaac
     3841 gggcactggg aaccccaccg tgagcccgac gaatgcggac ctccaggcgt tcgtggacgg
     3901 caagaacccg gccgtcaatc tcacctacaa ctcgacgacg catctgtggc gatcgaatcc
     3961 gctgacgatc cagccccaaa ccgggccggt cccgatcgag ctcaggtgga cgagcaactc
     4021 cagcagctgc ggcaagaacg gcaagtgcag cggcactttc ggcaacgtgc agcggagctt
     4081 cagcgggttg gacgaccgct cgggccccat acagctcctg caggtctggg agggcctaca
     4141 gcaagacacg aacgcgttcg agatgtgcag ctccgttcag tcgagctgca cgcacaacct
     4201 cgtcgtgcac atggcgatca agggcagtct caagaacgcc gccgacgtga acgacccgat
     4261 tgtgagcctg cgcttggttg gcaacgactc gaactcgagc cgtaaccagt cactcgactg
     4321 cgatcccggt taccccaacc tcaaagagga gctcgcacac ggctgccggc ccgcctacgc
     4381 gaagaacacg ggccaggctt gccccggcag cacgacgacg ctgtgggcaa ccgctcagcc
     4441 ctggaactgc gtggcgatcc agaccggaac cgcgatcaac caggtgtccc agggcatgaa
     4501 cgagcggatc cagggatccg cgaattcgaa cacctgcgtg agcccgaaca actggagcaa
     4561 gttcggcacc gcccagtggg actggagcga tccgcgaatc acccctctct tcctcgtccc
     4621 ctacggatcg ttcggcggga gcggcagcgg cacattgccc gttatcgact tcgcctactt
     4681 ctacgtgacc ggctacagcg gccagggcaa tggcaccgat ccgtgtgccg gcaaggggga
     4741 tgaccccgcc cagcccggct acatcgtcgg ccacttcatc aaatacgtct cgagcctcgg
     4801 aaactccagc ggttccacgc cctgcgaccc gaacgcattc ggtggctgcg tggtccagct
     4861 caccgaatag gaggagcagc agtgcaagtc caaacacccc ccgcacaacg gcccaagaag
     4921 gaccgatcga agcgagacct gcgaaccctg gtctccacgc gcaacggagc gatggcggtt
     4981 gccgcgatat cggctctcat cgccggcgtg gtcctgttgg tgttcctgaa ccaatacaag
     5041 gacagcctca acggctccga caagccggcg accgtgctcg tcgcacagcg tctgatcgag
     5101 aaggggagct cgggagacgt gatcgccacg cagggtctct accagaccac gcgcatcccg
     5161 aagggccagc tcaaggaggg cgccttgtcc gacccgtcgg tgctgcgggg gaaggcagcg
     5221 gcccacgaca tcctccccgg gcagcagctg accacggctg acttcggtgc gagcacggat
     5281 ccgatcctcc agaacctggc cggcaaccaa cgggcgattt ccgttcccgt tgacaacgct
     5341 cacgggatga tcggcgacat tcacgccggc gaccgagtgg acgtgatcgc cgggttcaac
     5401 tggcagccga acggcatggc gaccgcgacg ccggtgacga aggtcctgct gcagaacgtg
     5461 ctcgtgctgc gcgctccgag cggcggcgct cggtcgtccg ggccgggcgg ctccgacaag
     5521 agcgtcgtgc tcgaggtgcc cgacggcaag acgggcgaga tcgcgttctc gtcggataac
     5581 ggaaaggtct ggatcgtcaa gcgtccgaag gcaggcgcca aggacacaag ccagggcatc
     5641 gtctcgaccg agaccgtcct cttcggtgtc aagccgatcc gcacggacag catccagaag
     5701 acgatcagcg ccgagatcgg aaaggccctc aagaaggccg cgaagggtaa gtgatgactg
     5761 aaccaatcaa aatcctcgtg gtcctggact caggcgtcga gcctgagctg attcagaccg
     5821 tcctcgccat cgagcctgag gtgcagatcc tcgcgatggc agacagtctc caccaggact
     5881 ggggccggac ggccgaggag tcggcagaca tcctcgtggt ggcctgcagc ggttactccg
     5941 accgggtgct gcacttcatc agcgccgcga ccgcggagca cgctgagcgt ccgctcgtgg
     6001 tcctctcgga gggagcggcg aacggtttcg tccggcgcgt gttcgaggct ggcgccgacg
     6061 acatcgtgtc actgcccgac tccccgtcgg cacatgtcaa cggagcgctc gcggaggaga
     6121 tcgtcttcac tctccagaag gcgctcgccc ggaagcgcgg ctcgatggcg acgctgcgcg
     6181 acgcgctcgg cacgctggtc tgcgtgcttg gcccgaaggg aggcatcggc aagacgctca
     6241 cggcgacgaa cctggccgtg gcgctcgccg aggaagggga gcggaccgca atcgtcgacc
     6301 tcgacctcca gttcggtgac gtggctctcg cggtcggact ggcgccggag cgcacgatct
     6361 acgatctggc gacctcgagc ggcgctctcg acgcggagaa gatcgaggcg tacatggctc
     6421 aacacgactc gggcgtgtac gcgcttctcg cacctatccg gcctgaccag gcgagcgctg
     6481 tgaccgtgga attcctgcgt gaggtctacg agttgctccg tgggagctac gactacgtgg
     6541 tcgtcgacac gccaccgggc ttcacgccgg aggtgatcgc ctcgatcgac gcgtcctcgc
     6601 acgtctgcat ggtcgggacg ctcgactcgc tgtcactcaa gaacacgaag ctgggtatcg
     6661 aaacactcga cctgatgggc tacgaccagt cgcgcgtcag cgtcgtgctg aaccgcgcag
     6721 actcgcgcgt gggcataacg cgcgaggacg tcgccgaaat cgtcggccgc ccgcccgatg
     6781 tccttgtgcc gagccatcgg gacatcgcgc gctcagtgaa ccaaggcgcg ccgatcgtgc
     6841 tctctggcaa gcgttcggag gcggcgcggg cgttcgccga cctcgctggc ctctacatca
     6901 ccaatcgcag ccacgccctg gcgcccgcgg ccaacggcgc tgcggacgcg aacggccgcc
     6961 acaacggaaa gccccggaag cgccggtctc ggcggctgtt ggggaggcga ggctgatgga
     7021 gctacacgag cggctctact cgcagcggcc ggcggagcaa ccgccccagc gcgacctgtt
     7081 cgccgagctg aagaaccgca tccatgtggc ggtcatcggg gagctgggac cgcagctctt
     7141 caacacggac atggacccgg cggtgctgcg cacgcgcgtc atgtccgaca tcgaggggca
     7201 cctgtcgcag gaggtggggc tcgcgagacc ggatcgggtg aggctctcta acgagatcgc
     7261 cgacgacatc ctcggtcatg gaccgctgga gggaccgctc gcagacgaga gcgtgaccga
     7321 ggtgatgatc aacggcccct tcgacgtctg ggtggagcgc cagggcaggc tgcacgagac
     7381 gtcgattcgc ttcaccgacg agtcgcacct ccgccggatc atcaacaaga tcgtcgccca
     7441 ggtcggccgc cggatcgacg agtcgtctcc gatggtggat gcccgtctac ccgacggcag
     7501 ccgcgtgaat gcggtgctgc ctccattgtc cctgtcaggg ccgctcgtca ccatccgcaa
     7561 gttcggcaac aagcgctggg atctgaacga cctcgttcgg ctcggcacgc tcagccggga
     7621 gaccgtcgag ttcttcgaca agtgcatccg ggcagagctg aacatcctga tctcgggggg
     7681 cacgggctcc ggcaagacga cgctgctcaa cgcgatgtca accgcgatcc cggatgacgg
     7741 tcgcatcgtc acgatcgagg atgcggcgga actccgcctg aaccagcgcc acgtgctccg
     7801 gctcgaggcg cgcccgaaga acatcgaggg cgagggcgag atcccgatcc gtgcgctcgt
     7861 ccggaactcg ctgcgcatgc gacccgaccg catcatcgtc ggcgaggtcc gcggcgcaga
     7921 ggcgctggac atgctccagg cgatgaacac cggccacgac gggtcgctct gcaccgtcca
     7981 tgcgaacagc ccccgcgatg cgctctcgcg catcgagacg atggtgctga tggcgggctt
     8041 cgacctgccg gtgcgggcca tccggcagca ggtggcgtcg gcgctcgacc tgatcgtgca
     8101 cctcgagcgc cttcatgacg gctcacgcaa ggtcacggcg atcacggagg ttcagcgcat
     8161 ggagtccgac gtgatcacgc tgcaggacat ctacacgttc cacgttgagg gggtgacctc
     8221 gggcagcgcg gtgatcggca agatggtcgc cacggggttg cggccgacct tccagcagaa
     8281 gttcgagaag cacggcatcg agcttcctct cgacatcttc gcgaacggcg tgggctcgcc
     8341 cgagatgctc aggggcgcag gactgtgagg cgtctggccc tcgccgctgc gtgcgcgcag
     8401 ctcgcccttg cggtcttcgc ggcaagtgtg ctcgcggcgg cgccgacgct cacaccggcc
     8461 ggcgggctcg gcttcccgaa ccggcagttc gtgctctccc tgccgcccgg ctccggattc
     8521 gatccatcca ggatcgacgt ccgcgagaac ggcaggccgg tggatgcact gtcggtgctg
     8581 ccggccggcg cggcgggatc ccgtcagctc ggggtcgtcc tggtgatcga cacgagccgc
     8641 agcatgaagg gcgacgccat cgccggtgcg atggcggcga cgcgagcatt cgcgagccat
     8701 cgcaatcccc agcagcagct cggcgtcgtg accttcaacg ggacggcgtc cacgcttctt
     8761 ccgctgacga ccgatcaggc cgccatcgac aaggccctgg ccggcacgcc agccctcggc
     8821 ccgaacacgc acatctacga cgcgaccgag gcggcgatca gcctgctcaa ggccgcgcac
     8881 atcagcgcgg gctcggtcgt ggtgctctcg gacggctccg acacgggcag tggcgccacg
     8941 aacgcctctg tggtcgccga cgccaaggcg gcaggcatcc gtatcttcgc ggtgggcctg
     9001 cgctcgggcg cattcgatcc ggccactctg agcacgctcg cgaagggagg tgcaggaagc
     9061 tacaccgagg ctggctcgaa ggaggagctc gccaagatct acgacgagct gggcgccgag
     9121 ctgtcgagcc agtacatcgt gcgctaccgc tcgtcggcgg ctccgcacgc gaacgtacat
     9181 gtgacggctt cggtgacggg cacccctctc caggcgacgg ccgcctacaa cacgcccgcc
     9241 ctgccggtga acccgccgcc gccatatcac cgcagcttct ggcagtcgcc gggcgcggtg
     9301 gcggcgatca gcgtgctctg cgcgggtctc cttggcctcg ggctgctcac ggtgctcacc
     9361 tccagccctg ccaagcggag cctgcgggcg cgcatgcgcg acttcgtgtc cagtcccggc
     9421 gaggaacgcg gcccgcgtga acgcgtcacg tcacgtctgt tcgtgggcgc ggagaagacg
     9481 ctcgagcgaa ggcagtggtg ggcgacgttc aaagaggagc tcgacgtcgc ccgcatcgac
     9541 atccctccgg tgcgcatcct gcttggcact ttcacggcga ctgtctttct gacctggctt
     9601 ctctggttca tcaccggcgc cgcggtcgtc gccgtggtcg ggcttgcaac cccccttgcg
     9661 gtgcgcttcg cgatcaagcg gaagctcgag cacgagcgcc gtctcttctc cgatgagctc
     9721 gccgacaacc tgcaggtgat cgcctccgcg atgcgcgcgg gccagagctt cgtgggcgcc
     9781 ctttcagtgg ccgtgcagga cgcgccggag cccgcccgcc gcgagttcga gcgcgtcgtt
     9841 gcggacgagc gcctgggcgt gccgctcgaa gagggcctcg gagtggtcgt gcggcggatg
     9901 gccaaccacg acctcgagca ggtccagctc gtcgcggcgc tacagcgcca gaccggcggc
     9961 aacatggccg aggtactcga ccgggcgtcc gacaccatac gcgagcgcgc cgagctgcgc
    10021 cggatgatca agacactcac cgcacaaggg cgcctctcgc gctgggtcgt ctccgctctg
    10081 ccgccgagca tggtgctgat cctgtcggcg atcaaccccg gctacctcga cccgctgttc
    10141 aagagcaccg gcggtctcac cgtgctcgtg atttcggcgg gaatggtcgt gggcggctcg
    10201 ctcgtgatca agaggatcgt ggatatcgag gtgtgaggag agcgcgatga ccttgatcct
    10261 gctcacaggc ctgatcctcg tcggattcgc ggtcgcgctc accacgcgcg cgctcacgta
    10321 ttcccgaacc gacgcggcga ggcgactcgc cgagatccag agttacggct tcggcgccgg
    10381 ggccgtggag gaggcgccgc cgccagcgag cctcggctcc gctctcgacc acaccgccgg
    10441 ccgactcggc ggatgggcgg cacgccacct cgggagcttc cgcgaggacg acgtgcgtaa
    10501 gcagctcacg atcgcgggga tctactcgct cacgccgatc accttcctcg gctaccgggt
    10561 gctctgcacg gctctcttcc cggccctgtt cgtctggtgc tcgtccgcgc tgggcctgcc
    10621 ggcgctgctg gccgtgatcg gaacgctggc gtcgttcgcc gtgggctggg tcgcgccgat
    10681 gacctacgtg cgccggcgcg cgcggcagcg cttcgacaag atcgagcgtg atctgccgga
    10741 gctgatcgac cttctggtgc tgacggtcga agccggggtt ggcttcagcg gcgcgctgca
    10801 gctggcggcg caacgcctgc gggggcctct tggcgacgaa cttcgcttga ctctgcagga
    10861 gcagagcatg gggctcgcca ccaacgccgc gctcgcgaac atgctcagcc gctccgacac
    10921 accgtccatg cgctcgttcg tgcgatcggt gatccaggga gagagcctcg gcgtgtcgat
    10981 cggccagatc ctccgcaatc tcgccaccga gatgcggaag cgccgccggg cccatgccga
    11041 ggagcgtgcc cagaaggcgc cgatcaagat tctcttcccg ctcgtcttcc tgatcttccc
    11101 gccgatgttc gcggtgttgc tgtacccggc tttgtccacg ttctcgaaca ccttcggctg
    11161 atgaggcgcg cggcaataac gagcgtgttc ctgctgctcg cggggccaac ggcggccgcc
    11221 gccggagagc gcgtggcctc cgccatcgac cacccggtct tgcgcgagtg gctggtcgcc
    11281 ggctactgga cgctcaaggc ggcgatcgtg atcgcgttcg ccacgttcgt cttcacccgc
    11341 ggcccggcga ggcggccggc gcgcgagccg gtggcgttcg cggcttgcac ggtcgcgatc
    11401 gcaagcgcga tggctcttcg gagcccgagc gcgtccgacg cgaccgcggt cgtgctcgcc
    11461 ggcgaggtcg tgagcttggt cggctgcgcc tggcttttcg cgtcggcgat ggcgctggga
    11521 cgctgcttca gcgtgctgcc cgaggcgcgc ggactcgtga cgaacggccc ctttcgtctc
    11581 gtccgccatc cgctctacct gggcgagatg acgatcggcg caggactcgt gatcgcatcg
    11641 ccgagcctgt tcaacctcac gttcgcatcc gccttcgtcg gctcgcagac ggttcgcatg
    11701 ctgctcgaag agcgggcgtt gacggcggag ttccccgagt acgccgacta cgccgcctcc
    11761 acacctcgga tcatgccggc gctgtcgccc ctcgtcggcg gtccgaggac gcagagggag
    11821 cagcgcgcat gaccgcgatt ccggcgctgg gccgacgtct cggacgtgtg ctgagcgaca
    11881 cgtccgagac gagcgagcag cccggcgcca tccgcatcag gcgcgcgatg gccaccctgt
    11941 tctgcttcta cgggaccgtg ctgtgcgtca tggcggtcac ccggggcgaa gtcccgtcgc
    12001 tcagctacgt gatcatcttc atggcagccg cgtcgctctg cgtgaaccgc ggcggctcct
    12061 tcgtgcgcta ctggctaccg gtgctcctcg gcctgtacgc ctatggggtg gcaggggcat
    12121 acgcgcagaa gctcaagttc ggcgtgcatt atctgccgca gatcgacatc gagcgcgtga
    12181 tctcactcgg cgggacgctt cccaccacgt ggctccagga gcacctctat cacgggcaca
    12241 cggacgcgct cgcgatcgcg gcttcgatca cgtacttgtc ccacttcttc gtgccgttct
    12301 tattcgcctt ctacttgtgg tggcgccaac gcaaggacgc gttcacggcc ctgatgttcg
    12361 gcctgctgac ggtctcaatt ctcggtgaga tcacattcgt gctggcgccc acagcgccgc
    12421 catggatggc cgcacaggag gggttgatac cgccggtcca ccacgtgatc aagcaagcgc
    12481 tcttcgacat gcacttcacg aaggccgctg agttctacgg caactcggcg aactacaaca
    12541 ccgtggccgc gatgccctcc ctccatgccg cgtggccgat tgtgtcctac ctggtggccc
    12601 gcaattacgg cctgccgcgc tggacgcgtg ccgtgctggc tgcccagtgg ctcgcgatcg
    12661 tgttcgcgat cgtctacacg ggggagcact acctggccga cgccgtggta gcggtcccat
    12721 acgcgctggc cgcctcctgg ctcgtgggca aggcgctcgt gcggtcgcgc gggcacgaga
    12781 agggtggaag tcctgtaacc gagagtggcc tggcgctgcc gggcagcgtc cccgcgccgg
    12841 tcacgagtcc cggcgcccta cccgaaccag tcgagggatg accgatgcga attgagatcg
    12901 atagtcgctg ggttgcggcg ggcggggccc aggacgggca ggcgctcgtg gagtacgcgc
    12961 tgatcacgag catcgtgtcc atcgtggcca ttgcgatcct cgggaccatc gggagcgatc
    13021 tggtcacgct catcacgagc gtcgccaacg ccttctaggc gatggtcgac gcgggaccaa
    13081 gcaccggcgc tggtgcccgc cgcggagcag acgccgcttc accgtggcgc gtcgagggca
    13141 gcgattcgcc gcccgccgcc acgcggaagc gcgggctgcg cggctggcgg gcgcccggcg
    13201 gccgccgttt ctggctgctc gtggcgctgc tgctctcggt gaacatctac gtgagcgcgc
    13261 agataacgaa ggggccggag cgagtcggcg tcccctacac cttcttccgc gaccaggtgg
    13321 aatcagggca cgtcgccgac gtgaccacgc ggggcgacat gatccagggc gagttcaagc
    13381 cgcccgtcgc gtaccccgcc ggatcgaagg acaacgccac acgcttccag accacgcggc
    13441 cctccttcgc cgacgacgac ctgttcgggc tcctggtgaa gaagaacgtg actgtgaacg
    13501 ccaaggcgat cgaccaggga cggccgctgt ggctcgttct gatcacgtcc atcctgccga
    13561 cgctcgcgat cgtggccctg ttcctgatcg tgagccgcag catgggcggc ggccttctca
    13621 ggctcggccg ctcgcgcgcc aagctctacg acgcgagcgc gcaccgcacg aacttcgagg
    13681 acgtggcggg aatcgacgag gccgaggagg agctcgccga gattgtcagc ttcctgcgcg
    13741 atcccggccg ctactgggag ctcggcgcag aaatgccgcg cggcgtgctc ctctccggcc
    13801 tgcccggcac cggcaagacg ctgctcgccc gcgccgtggc cggcgaggcc gacgtgccct
    13861 tctactcgct gtccgcctcg gagttcatcg agatggttgt tggcgtcggt gcgagccgcg
    13921 tgcgggacct cttcaaccag gcgaagcaga acgccccggc gatcatcttc atcgacgagc
    13981 tcgacgcgat cggccgggct cgtggcggca gcggcgggcg cgcggcaggc cacgacgagc
    14041 gggagcagac gctgaaccag attctcgtcg agatggatgg cttctctggc tcagagggcg
    14101 tgatcgtcct cgcggccacc aaccgcccgg agatcctcga cccggctctg ctgcggcccg
    14161 gccggttcga tcgccgcgtc acagtgaacc cgcccgacgc cgccgggcgc gagcgcatcc
    14221 tcgccgtgca caccaggcgc gtcccgctgt cagccgacgt tgacctcagg caggtggccg
    14281 gtggcacgtc tggcatggtc ggcgccgagc tgaagaacct ggtcaacgag gcggcgctcg
    14341 cggccgctcg ccgcggcagc gccgaggtct cgctcatcga cttcggcgag gctctcgaaa
    14401 agatcgtgct gggcgcggag cggcgcataa cgctctcgcc atcggaacgc gagcgcaccg
    14461 cctaccacga ggccggtcat gcagtgctgg ggatgctcga gcccggcgct gaccaggtgc
    14521 ggcgcgtctc catcgtgccg cgagcacagt cgctcggcgt cacgttccag agctcggaga
    14581 ccgacaggca cgcctatgac gccgactacc tgcgcggccg catcatcggc gcgctgggcg
    14641 gccgcgccgc cgaggagctc gtatacggga aggtgaccac gggcgcagag tccgatctcg
    14701 cgcaggtcac ccgcatcgcg cgccagatgg tgggccggtg ggggatgtcg aaggccgtcg
    14761 gactggtctc ggtcgtgccc gggcccggcg aggagtcacg ggtggtcgcc gacgccggca
    14821 tggcgttctc tccggcgacc ggcgagctgg tggatctcga ggtccgccgg atcgtggccg
    14881 agtgctatga agccgcggtc caccggcttc gcgacaaccg gcctcgtttg gatgcgctgg
    14941 cggccgctct cctcgagcac gaaaccctcg acgaggaggc cgcctactcg gcggcgggct
    15001 tcgagccggg acgcgtagcc gactcaggtc acgacggcgc ctcggcggca gacgcgaggg
    15061 cttccgcgtt ctcggcggcc gccctcgtga cgccgaaatt ggggcagcaa tgatttgcct
    15121 cttgacgcgg tggtcagctc gggttacgtg tagatggacg taccgcaaaa gtggtacgcc
    15181 agatctcccc caaggaaggg tcactggctc tcccaccggc gcaatttgcg tacagcggga
    15241 tacgagccgg agcgaggcga cagggagggc gtgacgtgaa ggttctgttg gcggactcgg
    15301 accgcttgac cctccaaggc atcctgcggg cacttgaggg agtcgatgac atcaatgtcg
    15361 tcggcgaggc gcgctcgggc ggcgaaatgc tgcggcttgt ggagcgcgtc aggccggaca
    15421 tggtgctgat ggatgcccac ctgccggaca tcgacgggct cgaatgtcta gacctcatgc
    15481 gccggcgcca tcgcgacgtc aaggtcgttc tgctctcggc ctccgatggt cgggagcgca
    15541 ttcaggaggc gctcgaccgt ggagcgacgg cttacatcgt caagtcggtg aacccaaccg
    15601 atctcccgtc ggcgctgcgc caggcgttcg aaggaacggt tttcagtggg ttcggtcgcg
    15661 tgaagtcaga ggcaacagcc ggctcgcccg cggagggtgt tctcaccgag cgggagctcg
    15721 ccatcctccg cgcagtggcc agcgggctct cgaacaaggc gatcgggcgg gagctctggg
    15781 ttaccgagca gacggtcaag ttccacttga ccaacatcta tcggaagctc ggcgtggcga
    15841 ataggacggc cgcggcgcag tacgcgcatg ccaacggctt gctcggcgca agcccgggaa
    15901 gcgaacccgc gcgcgcaggt cagatccgag gcggtgaggc gggcttccgc acggcccgac
    15961 caagcatgct ggacagagtc ttgccagcga gataactgcg cggcctcgag cgcggcaggt
    16021 tgccgaccgc catgctcagc tcgaacgcgt tcctcgccgg tcgttctcga tgtcgcgcgc
    16081 gcctaatttg cagagtcccg cccggattta ggtagatttc gccgcacatt tcaggctgcg
    16141 caacccagga ggctccccat gtcggtcatc gatgagctcg tcgacgcgaa cacgcacttc
    16201 gcagatgccc tcggcggggt cgaaagcccg gcgctcccac cgcgtcgccg ggtcgcgatc
    16261 gtcacgtgca tggacgcccg gatcgtcccg gcgcgcatct tcggcctcga agaaggggac
    16321 gcgcacgtga ttcgcaacgc cggtggcctc gcccgcgagg cgctccggtc gcttgtgatc
    16381 agtcagcgcc ttctcggcac gagcgcgata gcggtcgtac accacacgga ctgcgggatg
    16441 ctgacgtttc agaacgagga tgtgcgcgag aaggttcagc aagacctcgg cgccgacgcg
    16501 agcgacatcg acttcatgcc gttcgccgaa ctcgaccaga gcgttcgcga cgacgtggcc
    16561 ttcctgcgcg attcgccgct gatcgctcct gacaccgaga tccggggctt cgtgtacgac
    16621 gtgagcagcc ggcgcgtgaa cgagctcgcg tgacgcgcgt ctagcccgcg cgggccggca
    16681 cccagccgca gatcgacgtg ccggccagac gcaggaagcg ccagcacgcg agcggcgtgc
    16741 cggacgcggc gttcgagtcc ggctccggta gctcgtcgag cacgtccacc tcgtcgccct
    16801 cccgcgcgac ggcgcgcaca atcccgccgc cgtggacgcc ggtcgtgttc acacagatgt
    16861 acatggcgcc gccatagggc cgcttcgcgc tgtacgtcgc gtacgtgcgc gcgcgtccat
    16921 cctcgcccac gaatcgctcg tccgagcgga agccgggatc ctgcagcggc acgcgcgaga
    16981 gcggcgtgag gtcgcactcg ccgcggtcgg tccgcgccgt gtcgaccttg gcgcgaaccc
    17041 ggctgaagcc gacccggcgc gggagcgcat cgcgggacac gaacgcgcgg atccggaagg
    17101 gcacgatgct cgcgccgggc gccagctccg agcgatcacg tgcgtcaaac gcgacgagcg
    17161 cctggctgct ctcgagcgcg ccgtcggcca tagcgccgcg cccctggagc gagagacggc
    17221 cggtgcgctc gcgatgaatc tcgcggccgg tctccgggtc ggtggtaacg agaccgtcgg
    17281 cgtcctgttc gagcagcgcc agcgtgcggc cgagcgagtc gagcatcggc cagcggcggc
    17341 gtccgccgtc gtcggggttc tgctccagcc ccgggaacgg catccgcagg ttgagctcgc
    17401 gcccgcgcga gcgccagacg tcgatcgaca gatccgacgc gccgtttggt ccggcgcgct
    17461 gccgttcaag cacaacgcga gtatcgctga gtagagtcgc aacgtgagcg atgtgcaggc
    17521 ctcgctcgag gagtaccgca gcgtccgcca gacgctcgag aaggcggtgc tgccgcttgc
    17581 cacgtccgtc gatggacgcc gtttcagctt tcagacgtcg ctccacgggc tcgacctcga
    17641 agccggcggc tacgtgaagc tcgacggagc cgcctccagc cggctcggcc aggtgctctc
    17701 cctgaacctg agcagtcagg acggaagcgc gcccggactt ccaaccgtgc gcatccgggt
    17761 cgggacgggc gatggagtcg tgctcgcggg ggatgggcat ccgttccacg acgcgctggt
    17821 gagcccggcc aggccagagg acgtggcgac gtggctcgac cggatccgtc cagagcgcgc
    17881 cgcgatcgag gtgggggagc tggcgcttgc gccgggtgtc ccgttctggc tcgacgccgg
    17941 cggcttcggg cgccacacat tcatctgcgg acagtcggga tccggcaaga cgtactcgct
    18001 cggcgtcctg ctcgaacgcc tgctgatggg cacacgggtc cgggtcgtgg tgctcgaccc
    18061 gaactccgac tacgtacgcc tcggggaaat gcgggagaac gcggacgtag acacggcgga
    18121 gcgctttgcc accgtcgccc ccgacgtggc ggtccgcagc ggcgccggtg gtcccgacgg
    18181 catccgcgtc cgcttccgcg agttgagccc ggctcagcag ggggcgctgc tgcggctcga
    18241 cccgctcgcc gaccgagagg agtacgccga gctcttgagt ctggtggagg acgagggcat
    18301 ccgcagcgta gccgagctcg agggggtcga atccgacgcg ctgcgcctgc gcgcgcgcaa
    18361 cctcggtgtc gaccgctggg cgatatggcc cggccgcgag ggcgggtcgc tggcggcgga
    18421 gctcgaggac ccgaacggcg cccgctgcat cgtcgtcgac ctgggttcac tcggcagccg
    18481 cgaggagcag gcgatcacgg cgggcacggt gctcgagcgc ctctggcggc gacgcgcgcg
    18541 tcgcgagccg atcgtcatcg tgatcgacga ggcccacaac atctgccctg ccgagcccgc
    18601 cgacgcgatc accgcgatcg cgaccgagga cgcgatccgg atcgcgggcg agggccgcaa
    18661 gttcggtctc tacctgatcg tctccacgca acgcccccaa aaggtgcacg agaacgtcct
    18721 gtcccagtgc gacaacctcg tgctcatgcg gatgaactca atcgcggacc tggcccacgt
    18781 cggcagtgtc ttctcgttcg tcccgcccgg cctgctggat cgcgccacca ccttcggcca
    18841 gggcgaggca ctcgtggccg gcaagatcgc ttcacaccca gcagtgatcc gcttcgcgtc
    18901 gcggattacc cacgagggtg gggcagacgt ggcgggctgg gcctgacgtc acccgcggcg
    18961 tcggcggctc agatacatcg gcgacgtgag tgcgaggagc ggcatgccgt cacgctgcgg
    19021 cgtggcgttc ccaacgtcgg tggtggtgtc gaccgggagc gcctcgcgac cgagcaggat
    19081 cgctcgcacc cacccggtct cgcgcggcgc cgcgaagcgc acctcgccgc cgggctcgag
    19141 ctcgcgctcg agtatctgct gtccgttcgc acgcacgcgg acgaccgccg gtacggtcag
    19201 cccgggtgcg gtgacccgca tcgctgtccc gggcgcgacg gcgtcaccga tgatgccctc
    19261 gccgggcgcg tcgagcagta gctgcgctcc cccgagggag ggcggctgtc cggacacgct
    19321 cgtgcggccg gcgcgcagcc cctgaagcac gccggcgacg ctcgcctcgg gcgcgtatac
    19381 ccacgtggtc ggctgtcccg ggccggcgac ggcgtggagg gagagccagt gggaatcgct
    19441 gccgccggtg cccgcgattc gctctccacg gtcgagccag cactcccagt aggtctcgga
    19501 gacgtcgctg cgggcgacag acggattcca gacctcgatc gtgtccggcc gcacgtcgaa
    19561 cccataggtc cagttgatcg attggcagtc ggcgcagccg ccgctgccac acgagtggaa
    19621 ctctgcgtca agcggcagcc ggtaggcggg gtggttggcc tggaagacgc cgccggcccc
    19681 gcgcagctcg tccgccatcg cgttgatcac atcagctgcc tggtcgcctg cgtcgtgcag
    19741 acgcgtggcg ccgagcatct gcgcgtgacc cttgatcgag tgctcgtagg ccgggatggc
    19801 gatcaagccg cccgcagccg agccgagctc tgggaccgag cgcacgtcgt tgtgatcgga
    19861 cacagcgaga aagcggagcc cgcgttcgcg cgcctcggcg tacttgccgg cgaccggcag
    19921 gccgagcgtg tacgcctcgt cgggacccgt gttgtcgtcc atgggtccgc catagacgtc
    19981 atgtgagtac gtggagtgcg tgtgcaggtc ccccggcagc cacgtgcctg cggccggtcc
    20041 gggccgcggc gcaccgatcg cacccgagcg cgacagcgaa agcccgccgg cggctccgcc
    20101 cgctgccagt agcccgcgct gtaccagcac gcgcctcgtg acccctgcgt ccacgcgcgc
    20161 ggatgattac cagctcacgc gccccgtttg tcgcgattcg gtgaacaagc cgcggaataa
    20221 gcacgctcga aagaatcttg ctagcgagat acctggtact ctgcccgctg tgtccatcgg
    20281 tactcacgat cacgtcgacc gcgtcctgga cgagtggagc cgggaacggc cggaactcga
    20341 cacgaccgcc atgggcgtga ttggcaggct gatgctggtc gctcggctgg cggacgggag
    20401 gctgtcggcg ccgcttcggg agcgcggcct cgaccgcggc tggttcgacc tgctggcggc
    20461 gctgcggcgc gccggccgcc cctacgagct caatcccaag gcgctcatgg agtccgtgat
    20521 gctctcgtcc ggcggcatga cgaagcggct cgacaacctg gagcgcgccg gattggtcga
    20581 gcggcgcccc gacccggcgg accggcgcgg gacgctcgtc cggctcacgc cgtcaggccg
    20641 gcgcgcggtc gaccgcgcgg tcgagctcca catcgccaac gaggacgcgc tgctcgactc
    20701 gctcgacgac gacgaactcg gagcgctgga cgacctcctg cgcaagttgg tcgcggcgct
    20761 cgagcggaag gaggcggcgg catgacgccc acgacgatgc gagccgtcct tctacgcgag
    20821 cccggcggcc cggagcactt gggctgcgag caggtcgcga ccccggcgcc gggtcctggt
    20881 gaggtgctgg tgcgcgtcca cgcagcggcg atcacgcgcg gcgagctcga gtggcccgtc
    20941 gaccggcttc cggcgatccc ctcctacgag gtgtccggcg tggtcgcggc actcgggccg
    21001 ggcgtcgatg acgtcgccgc cggcgacgag gtgtacggga tgaccgcgtt cgaccgcgac
    21061 ggtgtcgccg ccgactacgc cgccattccg agcgagctgc tcgcgcccaa gccgcgcgga
    21121 ctgagccacg ccgaggcggc ggccgtcccg ctggcggctc ttaccgcgtg gcaagggctc
    21181 ttcgaccacg gcacgctggc gccgagccag cgtgttgtga tccacggggc gacgggcggc
    21241 gttggacagt tcgcaacacc cctcgcgaag cgccacggcg cccacgtgat cgccaccgcg
    21301 tcagccgacg cggccgagcg cgcccggcag ctcggagccg acgaggtcgt cgattacacg
    21361 gtcggacaat gggcgacggg catcgaaccg gtccacctcg tgttcgacac cgccggcggg
    21421 gagcggctgg cagcgtcgcc ccgggtggtg cggagcggcg gtcggctcgt gtcggtggcc
    21481 gaggagccac cggaggcaac cggtatcagc agcgtctact tcgtcgtcga ggctagccgc
    21541 gatcagctga cggagatcgc gcggctgctg gacgaaggct tcccggcgcc ggcggtcgac
    21601 tccacattca gcctcgagga ggcgaagagt gcgttcgagc gcagcatggc cgccgacaag
    21661 cgcgggaagg tcgcgctcac gtcgcacgct gagaaggcct agacccggcg tccgacggcg
    21721 tcagtcctcg ctcgcgaggc cctcggtcgc gtttcggccc gcctcgaccg tgtggcgcag
    21781 gaagtcggcg atcacctcga gctgctcatc cgagtagtcg gacaggatct tgttcatcga
    21841 gcggctcatc gcggagtaga gcccgagcag ctcgccgtag cggtcgtgca cgacgcggac
    21901 cacgacggct cggcggtcgt tggggtcgcg ctcgcgcacg atccagccgc cgcgctcgag
    21961 ccggtcgagg atgccggtga gggtcgccgg gtggagcccg gctcgacggg ccagctcgct
    22021 ggggctgagg ggtccggagg tatcgaggat gtcgagacag tcgaggtcga cgtccctcag
    22081 cccgacctgc aggccgacgc ggtagttgag cagagcgagc tggatgcgta gctcgcgcag
    22141 ggcgttcctg acctcgttcg ccgctcgctg ccgctgccgg atttcctcgc ggcgctgagg
    22201 tgatacggat ttcgtttgtt ctgggttcaa tatgctatgg ttctcatatc gtatgtagtc
    22261 gataacactc tactcccccg aggcccgcct tgtcgactcc cgcttccaca ccctcattcc
    22321 tcgcgacccg ccgcggcaag ctcactcttg tcctcctggc cgcgatcgcg ttcctcgact
    22381 tcgtggacgc gtccatcacc aacatcgctc tcccatcgat caggcacgac ctcggcgtct
    22441 ccgtgcaaga tctgcaatgg gttccgagcg gctacctcct gacgtacggc ggcttcatgc
    22501 tgctcggggg ccgcgcggcg gaccttctcg gacgccgtgc cgtcctcgtt gccggaatgt
    22561 ccgtgatcgg catctcgtcg ctgatcggcg ggatagccgg gagtcccggc gtactgatcg
    22621 cctcccggct cgcacagggc ctcggcgctg cgatgacgct gccagccgcc ctctcgattc
    22681 tcacgaccac cttcaaggag ggctcggacc gcaccaaggc tctcgggatc tggggcgctg
    22741 ccggtgccct ggcttcggct gcaggcgtga tgctcggcgg cctgctgacc gagggccccg
    22801 gctggcgctg ggtgttcttc gtgaacccga tcgtggtggt gctcctgctc ctcgcgacgt
    22861 tccgcctcat cagtggcgag cgtcccaacg cgcagcgtct gcgcaacttc gacattccgg
    22921 gcgccgtgct ggcgacgggc gggatgctcc tgctcgtcta caccttggtg aaggcgccgg
    22981 acatcggctg gggcaccggc cgcacgatcg ccgggctcgc aggcgcactc gcgttgctcg
    23041 ctacgttcgt cgtcaacgag ctgcgtagcc gcaacccgat cgctccgctc tcgatcttca
    23101 ggatcaacgg gctgggcttc gcggacgcga cgcagctcat cgcattcgcc ggtttcctcg
    23161 cgatgttctt cttcctgacg ctgtacatgc agaacgtcct cgggctctcg gcgatccaga
    23221 cgggagcggc ctacctaccc ctgacgttcg taggcggcat tgccgccggc gcgtcgtcgc
    23281 aactcgtgtc tcgcgtcggc acgcggcccg tcatcgtggc cggggccgtg atctcggccg
    23341 ccgggctgta ctggctgtcg cgcatcccgg ccgacggttc gtacttcggc agtgtgcttc
    23401 cgggcatgct ggtgctgtcg ctcggcttcg gccccgtctt cgttggagtg acgacggcgg
    23461 cgaatgccgg agtgccggcc gacaaggcgg gcctggcggc cgcgctgctc aacgcctcgc
    23521 agcagcttgg cggcgcgctt gggctggcga tcttctccgc cctggccacg agccgcacgc
    23581 atgacctgct ggccgcgcac acgccaccgg cggaagcgct caccagcggc ttccagcgag
    23641 cgctgctcgc cggaagcatc ttcatcctcg cctcggccgt gatcgcgctg cgcgcgacca
    23701 acacgcgtgg cgagacaccc gacgccgcgg cgcacaggcc cgtgcccgta cccgaaggag
    23761 cgtgattgcg cgctagcgcc tagagccggg caatgacggg tgtgccgcct agggtgctgg
    23821 cccgaagcgg cacaccccgt tgtagcaagg gtgcatttct tctataccaa ggcgtcgccg
    23881 caaacgagat ggatcttttg cacctatgcg gtgcataact tccatctcga tctcggccgg
    23941 cgcgccgcga tggatgaaat gcacccgccc gcgctgagcg ccccggctac tccgcggtcg
    24001 ggaaatggag gtgcccgcgc tcctgcgcgg cgccgggtac ctcctccggc cagcgactcg
    24061 tgaccacctt cccgcgcgtg tagaagcgga ctccctccgg cccgtggatg tgcgtgtcgc
    24121 cgaacagcga ctgcttccag ccgccgaacg agtggaacgc catcggcacc gggatcggca
    24181 cgttcacgcc gaccatgccc acctgcacgt cgcgctggaa gcgacgcgcg gcgcggccgc
    24241 tgctcgtgaa gatcgccgcg ccgttcgcga acgagttcgc gttgatgagc gcgatcgcct
    24301 cgtccaggtc ctcggcgcgc accatcgaca gcaccgggcc gaagatctcg tcgcggtaga
    24361 ccgacatatc tggcgtcacg cggtcgatca gcgtcggacc gaggtagaag ccgccgctgc
    24421 cggtctcgag ctcgcggccg tcgaccacca cgtcggcccc gtcgtcggcg ccctgctgga
    24481 tgtagccggc gatgcggtca cgcgcctggg ccgtcacgac gggacccatc tccgattccg
    24541 ggtctagccc cgggccgacc ttgatcgagc gcgccctctc actcagctgc tgcaccagct
    24601 cgtcgccggc cggaccgacc gcgacgccca ccgaaatggc catgcagcgc tgaccggccg
    24661 agccatagcc cgcggccgtg agctggttgg cggcgaactc gaggttggcg tcgggcagca
    24721 cgatcgcgtg gttcttcgca ccgccgagcg cctgaacgcg cttgcggttc tcggtcgcgc
    24781 gcgcgtgtat gtacttcgcg accggggtcg aaccgacgaa cgagatcgcc gctacgtcct
    24841 ggttgtcgag cagggcgtcg acggccacct tgtcgccgtg catgacgttg aagacaccgt
    24901 ccggcaggcc cgcctccgcg tacagctcgg cgaccaggtt cgaggccgac gggtcgcgct
    24961 ccgacggctt cagcacgaac gtgttgccgc tggcgatcgc catcgggtgc atccacatcg
    25021 gcaccatcac cgggaagttg aacggggtga tccccgcgca cacgccgagc ggctggcgga
    25081 acgagaacga gtcgacgtcg gtcgagatct gctccgagaa ctcgcccttc agcagctgcg
    25141 ggatcccgca ggcgtattcc acgacctcga ggccgcggat cacctcgccc ttggcgtcct
    25201 cgacgatctt gccgtgctcc gacgccacca cccgcgccag gtcgtcgacg tgccgctcga
    25261 ccagctcgcg gaaggcgaac atgatcttgg ctcgtctggt gacggacacg tcgcgccagc
    25321 tctcgaacgc agcgcgcgcc gccgctaccg cggcgtcgac gtcctcgcgc tcagccagca
    25381 gcacctcggc ctgctgctcg ccggtcgccg gatcccagac tggggacgtg cgagtcgagc
    25441 tgccggacgt ctcctggccg ccgactcggt gctgaatggt tctcacggtg gtggtgctca
    25501 tcgcctgatc gctttccgcg ggatttcaga aggggttgac ccccgatgct aactggagcg
    25561 ttccagcgat gggaggggcg gcccggtccc aaaaggggcc gggccgtgtc catcaccaac
    25621 gagcgccgcg aggcgcccgc cgaatcactc tggcgggatc tccgcgtaga cccggtcgac
    25681 gttcacgccc tcgttgcgcc tgatcgcccg ggcgccgtag taccaggcga gtccgaacgc
    25741 gatcgcgccg ccgagccata tccacagttg gctcttgtcc ttcagcccga agaagctgta
    25801 gtggaagtaa cagaagtaga ggaagaccgt ggtgaggatc gcgccgatcc ctgtgatcac
    25861 caccagcgga atcccggcca ccgtcttcgc cgacaccgac gcgcggtaca gctccggctt
    25921 gcggtacggg aacgagatcg cggagatcgc gacaagcgtg tgcgtgatca gctggatcaa
    25981 ggtcgcgtac acgaccacct ggaagaagct gttcgagatg aagatcgccc acgcgtacgc
    26041 ggcgatggcc agcacgatcg tgatgatggt ggccgtcaac ggcacaccgc gccgagacac
    26101 ctgggtaacg gccttcggcg cgatgccgtc gaaggaccag gcgaacagcg tgcgcggcgg
    26161 ctggagcgcc gtctcggcca tgatcaccgg ccagaacagc acgaacgaca ggcagaggaa
    26221 caccgcgaac acgatgccgt tcacctgagc cgaggtcagc acgaagtagg cgcccgacgt
    26281 gccgagcttg tcgggtagtc cgccgccgaa cgcggcggtc atgaactgcc ggccgaagct
    26341 gttgaagaag atcgcgacga acacgatcac gctcaacagg atcagggcgc tcgccgcgcc
    26401 catacggttg gcggtcttca tcgtgttgcc ctggcgcagc tcgcccgcga agaacgtcga
    26461 ccagtaggcg tagatcgaga tcccggccac caccgcgacc attgggatcg tctgcgagaa
    26521 cgagaagcca gtgctcaggt cgaccccgtt cttctgggcc gtgctgagca ccgagtggta
    26581 ggtgtcggga ttgccggtga tcggctgggc gaacgagttg aagtgcccta tgaacgagct
    26641 cttcgacgtg aacagggcaa tcagcgcgga caccagcagc ccgagcagcg agaagccgag
    26701 caggccgaac atgaagcgct tcgcccacgt ccagccggcg cccactgcga tgccggcggc
    26761 gaggatgccg accacgccga gcccgaactg ccagccgtgc tcggtggcca ccgagttgct
    26821 ccacttcacg agcgtgccgc tgtcgccgat caacccgacg atcagcaacg ccggcgcgag
    26881 gccgagcgtg accattgcga gcgcctcgaa ggcgatcgac aggaagctcg acagcgagat
    26941 gcagaccgac gagacgactc ccgccgccgg gtgcaggatg cggctcacga gcacgtagtc
    27001 gccgcccgac ctcggcatcg cggcggtcag gaggccgaac gtgtacacca cgctcagcgc
    27061 gagcgggatg gtgagcagcg tcgcgaccag gaagttgccg cccgggaacc cggacaacgc
    27121 gccgaacagc gcgatgccga tcacgaacgg cggcgccccg gtgatgaagt tgatcgccat
    27181 cgcctgccac ggcttgacct cgcgcacgag acccgtggcg ttgcggacga acaggcctgt
    27241 ctggggggtg gttgttcgag ctacctcttc agtggccact gcaccctctc ctttggtcaa
    27301 ccgactacga actgatgtga gatgacgcgc tcgcggccca gcttcacgat caccccgtgc
    27361 agccggccgg tgttgtattc cgagccggaa ttgagggcga gagtgcggcc gatccacgcc
    27421 tcgccccgcg attcgtggat gtggccgtgg agaccgagca gcggctggta ctcctcgatg
    27481 atctgccgca ccgcgtggct cccgaccggg atctcgaccg gctgcccgcc gctgaaccgc
    27541 ggcgtcaggt cgggcttgag ctcggttgct ttgtcgagcc cgctgtcgta cggcgggacg
    27601 tggaggttga agatcgcccg ctccggcgcc tcgacctgct cggccaggct cttcaggcgc
    27661 cggtagagcg cctcctcgtc gagctcgcgc gggctgtccc acggggtcgg gttggcgtac
    27721 gagcacgaga tcatctcgtg gtcgcccacg cgcacgacgc ggtcgtcgca gaactcgatc
    27781 gcgtccgcgg aggcgagcat ctcgtcgacg taccaggggt cgtcgttgcc ggccatcacg
    27841 aacgcgtgcg tcccgttttc cggcaggcgt tcagcggcga gcgcgatcca gcgatcgagg
    27901 tcctcgcgca ccacgcggtc gaagagctcg tcctgtccgg cgccgtttcc ggcatagcgc
    27961 gccagctcct ccggcggtgc gatccacggg tacatcccgt tgaagcggat ggcgtcgacg
    28021 atttccgcca gctgctcgtc gtcgacggcg acacgctcgt cgcccagaaa gcggcacatg
    28081 tacgtgccgt cgggacggtg ctcgaccggc acgaccgcct tgcccacgag gtcgccgccc
    28141 atgatcacgt cggagagccc gtagaagcgc gccgctccga ggaacttgcg ccagcagcgg
    28201 tctgagccgt ggatgtcgga cgcgtagtag agctcgagcg cgtcctcgtc gggagcgtga
    28261 ccgcggcgcg agaacaggcg acgcacgtca ggtcacctcc tcgggctcgt cgtaccagcg
    28321 cacgcgctcg cccactcgcg cgcgaagctt ccagcgggtg ctcttcggcg cgtcgtcgat
    28381 acggcggcgc agctcgtcga tgcgcgaaac gatcacgtcc tgctgctcgg gggcgaggtc
    28441 gtaacgcgcg accgcgctgc ggatgcgctc gaggttgagc gtgcacgtgc gccacaggcc
    28501 ccagtcgcgc gagcagaggc tcgcgacgta gtcacagttg atcgcgccgt cagcgtcgtc
    28561 gtcggacagc gggcggtgga acaggagcgt cagcacgtcg cgctggtcct tctcgttgag
    28621 cgcgaacacc tgcagcttcg taagcagcag gtcggcgagc ggcaaggtgt cccgcgcgac
    28681 gagcagccgc tcctggatgg gcagctgatg ggacatctgg aaggagccga ggaacacgtc
    28741 gagctgccga ccgtgggcgt tgtcgtagta gaggagccgg cggtggccgt tcagcgcgtt
    28801 gaactgctgg ttgccctcgt agccgagcga gtccagcagt gctaccacct cgggtcccat
    28861 cccgcggcgc gcgacgaagt cgatgtcttt gtagtcgcgc ggcagtaacc ggttgtctcc
    28921 ggccaccagc gcgatcgcga gacccccgat cagccgcagc ggcacgtcac gcgcattcgc
    28981 tgcgtcgagc actctgcgcg cctcggcaac gatgtcagct cgcggggcga gctccgcgcc
    29041 gggcgcgtcg tcgctcatgc catcggctcc agcgcgccgg cgtacgcggc gtcgcgctcg
    29101 cgccggccga ccaagatcgc tcggctacgc ggtgcccacg cgcggatcgc acgaaatctc
    29161 gcgattgccc acatgtcgca gcgcgattgg agggttccag attcgcgccc agcaatcttc
    29221 taccgctcac tccgttggga gtcaagtatg gctccataac ggtcaatagt ggagccgctt
    29281 gacagcgttt ccgggctctc tttagagtcg tggaacattc catggtgcgt gcctcgtcag
    29341 ccctgcccga ggtcgaggac gccggctgag ctacgtggcc gccggcgcgg cggtcgagct
    29401 ccgcaccacc agcgccgggg cgagctgcgt gtggcgcggc gggccctcca gcgtgccgct
    29461 cagccgcccg agcagcgtgt cgacagcgat gcgggcgatg tcgtcgaacg gctgccggat
    29521 ggtggtgagc gagatgcgcg ccagcgacgc cagatgcacg tcgtcgaagc ccacgaccga
    29581 caggtcctcc ggcactcgca gtccgtggcg gtcggcgtac tcgagcaccg cgatcgcccc
    29641 gatgtcgttc gagcagaaga ccgccgttgt gtcccgaagc tggtcgagga gcgacaagtc
    29701 ggcgcccttg acggttgcct cctcacttcc cggcccccag tggatgggtg gcgccggatc
    29761 gacgccggcg ccccgcatcg ccgcctggta gccgcggtgg cgcgccacgt gcgcgcgccg
    29821 ctcgaggctg tgcgtggtga ggtatgcgat ccgacggtgt ccgaggtcga gcaggtgctc
    29881 ggtcgcgagc ttcccgccgg cggagttgct cacggcgacc gaatcgcccc agtcctcgcg
    29941 caggcccgcg aacacgaccg gcacaccgcc gagtgccgcg gctatgtcgg gcgagcggcc
    30001 gaagaaggcg aggaacacca cgcctgcggg gcgctgctcg aggagggcct cgacccggga
    30061 ggctgcgaag tccgcctcct cgtcgcccgc gccctcgatg ttgcagaaca tcgtggtgta
    30121 tccgctgcgg aacgcgaggc gctcgagtcg cttcgccaag tcgccgtaga acgggttgtc
    30181 gaggtcgcca acgagcacgc cgaggatcgt cgtgcgctgg ctcacgaact gtcgcgccag
    30241 gacgttcggc cggtagccga ggatctcgat tgctcgctcc acccgggcac gtgtcgccgc
    30301 ggacacgccg tcgtttccgc gcaccacgtt cgagaccgtc gatttcgata cgccggcaag
    30361 cgcggccacg tcgaagatcg tcgggttacg tcccggcgcg cgagactgcg ttgccttcaa
    30421 ccggcctgcc cctcctcgcc cgtcgcctcc ggccggccga gtgctggagt gctccacggc
    30481 agttttctac ccgtgcttcg cggacccgtc aagctacgca tccttgacag cgccgtgggc
    30541 gtcggattag atgcctggaa cgttccacca cggcgtccac cgcgtactga ggagaccgat
    30601 tggagagacg taagcgcgtc ggcgtgatcg gctgcgggct gatcgcgcag gtgatgcacc
    30661 tgccctacct gaccgagctg agctaccgct tcgagatcgc cgcgctctgc gacgtctcgg
    30721 aggaggtcct cgaggggtgc gccgagcgct acggcgtcac gcgcacgttc acgcgttggg
    30781 aggaactgct cgaggagccg ctggatgcag tgatggtgct caccgccggc aaccacgcgc
    30841 ctatggcgat cgccgccgcg cgcaagggcc tccacatctt cgccgagaag ccgatggcga
    30901 tctcggcctc cgagggcgcc gcgatgctgg gcgccgccga cgaggcgggg gtgcgcctca
    30961 tggtcggcac gatgaagcgc tacgaccccg catacgagcg cttcgtgcag ctcgctcccg
    31021 agatcgacga cctccggctc gtgcgcgtga ccacgctcga gtcgccgctc gagccctacg
    31081 tggcgcacta cccgatggtg cggccgagct cgccgctgcc gcccgagctg atcgcgcagt
    31141 ggaacgcgga ggaggcgccg atcatcgacg ctgcgatcgg cgcggacgcg agcgaggacg
    31201 accgctactg ctaccgcaac atcctgctcg acaacctcgt ccacgagttc aacatgctgc
    31261 gtggcctcct gggcgagccg agcgccgtca cgtacgcgaa cctcgcgccc cagtgcgtcg
    31321 ccgtgaacat gcgctttggc gacgccgagt gccatctctc ctgggtcgac ctgcccggca
    31381 tcgcgcgcta cagccagcag gtcgcgctct acggtcccga ccgccgcctc gtgcttacgc
    31441 tgccgtcgcc cttcctgcgc agcgcgcctt cgaagctcac gaccgagggc ggcgagcctg
    31501 gcacatcgca cggctgggat cgcgacgaga tcgtgtccta cgacgaggcc ttcaaacgcg
    31561 aactcgtgga gttccacgag gcgatgctcg agggacgcga cgcgcgcacc tcgggcgtgg
    31621 acggcctgag ggacctgatg ctgtgcgagg cgatcgcgcg cgtgcaccgc acccgcgagc
    31681 ccgtgaccaa cccgaccgag ctgcccgcgg cggtggccgc agaagtagga ggaagctgat
    31741 gacgcaggag atcggcgagc gccgcctgca ggaaccggcc accagcgaga tcgtggtcgc
    31801 gaacgcgccg gtgagctacg gcgctttcga gatcacggtc ggcgtctacg acaacgtgcc
    31861 gcagccgctc gagctgctcg acgccgtctc gagcgcgggc tacgagggtg tcgacctcgg
    31921 accgctcggc tacctcggca ccgggtccga acttgccgag cgcctcggga gtcgcgggct
    31981 gtcgctcgcc ggcggctact tcgagctgcc cttcagcgag cccgacaggc tgaaggaggc
    32041 gctgcccgag ctcgacgcgc tgctcgacgt gttcgccgcc gtccccgacg ggcgcgtgaa
    32101 ggcgcgcccg acgctcgcgg acgccggctc gcccgagcgc gccgcctacc ccggccgcgc
    32161 cgccaccgac cgcagcgtcg gccttgacga gaagggctgg aacaacttcg ccgacggcct
    32221 cgcgcgcgcc gtcgagcgct gtcgcgagcg cggctacgag cccacgttcc accaccacac
    32281 ggcgacgtac atcgaggctc agtgggagat cgagcggttg ctcgagctga gcgacgttgg
    32341 cgtgacgctc gacaccggcc atctcctgct cggtcgtggc gaccccgtga aggctgtgca
    32401 cgattggggc gagcgcatca accacgtgca cctgaaggac gcgcaccgcg acgtgctcga
    32461 ggcgatcgtg gccgaccgcg cgccggtcat ggagatctgg acgcgcaagg cgttctgcgc
    32521 gctcggtgac ggcgacgtgg cgatcgacga cgtgctctct gggctgcgcg aaatcggcta
    32581 cagcggctgg ctcgtggtgg agcaggacac gattcccgcg gcgggcgagt ccgtcgacaa
    32641 ggcagccggc gagcaggcgc gcaaccgcga gtacctgcgc gagcgcggac tgtaggccca
    32701 tggcggaggc ggtgcgcatc gccatggtcg gcgccggccg gatgggccgg gtccacctcg
    32761 acgcgctcac gcgatgccgc cacgccgagg cggtcgcggt ggtcgacccc gtccccgccg
    32821 cgcgtgacgc cgccggcgcg gccggcctgg cgggctacgc cacgctcgac gagctgctcg
    32881 acgcgggcgg cttcgacgca gtgctcgtcg cagcacccag cgaccggcat cggcggatcg
    32941 tgaccacgct cgctcgcgcc gggctgccgg tgctgtgcga gaagccctgc ggcacgagcg
    33001 ccgacgaggc agcggagacc gctaccgcag tggaggcggc gggcacgccg ctgcagatcg
    33061 gttactggcg ccgcttcgtg ccggagctcg ctgcgctgcg tgagcggatc tctgagggcg
    33121 agctcggcga gatcacgctc gtcgcgtgcc atcagtggga tcgcgagctg ccgcacccgg
    33181 ccttccgtgc gagcagcggc ggcatcaccg tcgacctcgg tgtgcacgag tacgaccagg
    33241 tgcgctggct gctgggccag gagttcgaga ccgtcgcggc cgtaccggca ggctcgagcg
    33301 acgcggacgc gggaccgacg gatcccgact cggccgcggt gctcgcacgt ctttcgggtg
    33361 gcacagccgc gagcatcact ctcgggcgcc atttcggcca ggaggactcg tgctggttcg
    33421 aggccttcgg gcgcgagagt tacgagcgca tcccgttcat gtggggcgat gccggccagg
    33481 aggcgttcgt cacggcgctg accgcgcaag ccgacgcgtt cgctgggctg gtgcgcggcg
    33541 agccgcttcg cggggccagc gcggcggatg ccgtcgctgc gctcgagacc gccggacgcg
    33601 tggccgaggc gcttgagcgc gcacccgcgg aggtcggctg agcggtggcg ccgttcgacg
    33661 tgatcaccac gggcagggtc ggcgtggacc tgtatcccca gcagtcgaat gtgggcctcg
    33721 ccgacgtgga gacgttcgcc aagtacctcg gtggaagcgc gacgaacgtc gccgtccagg
    33781 ctgcgcggct cggcagtcgg agtgccgtcg tcacgaaggt cggcgacgac ccgttcggcc
    33841 cgttcgtgcg ccgcgcgctc gaggagttcg gcgtcgaccc tcgctgggtc gggaccgatc
    33901 ccagcctgcg cacgccgatc gtgttctgcg aagtccaccc gccggataac ttcccgctgc
    33961 tcttctatcg cgagccgaag gcgccggaca tgaatttgac tcccgaggac ttcgacgccc
    34021 aggccgtccg cgacgcgacc gtgttctgga ccacgggcac cggcttgtcg gacgagccga
    34081 gccgcagcac gacgctcgcg atgctcgagg agcgaagcgg tgatggggcg cgcggcatca
    34141 cgatccacga cctcgaccac cggccgatgt tctgggagga cgaagccgac gcgacccgct
    34201 gggcgcgcga ggcgctcagg cacgcgaccg ttgcagtcgg caaccaggac gaggtggcgg
    34261 tggccgtcgg cacccgcgac cccgacgagg cggcgcaggc actgctcgat ctcggtgtgg
    34321 agacagcgat cgtgaagcgc gggcccgagg gcgtgctcgc gcggacacgc gaagagcgcg
    34381 tggacctgcc gcctgtccgc ctcgaggttg tgaacggact tggtgccggc gacgccttcg
    34441 gcggcacgct ggcccacggc ctcggtcacg gctgggagct cgagcgcacg atccgcgcgg
    34501 cgaacgccgc cggcgcgatc gcggcgtcgc gcctcgcgtg cgctgacgac ttccccacgc
    34561 tcgcagagat cgatgaggtc ctcgccgctc atgcctgaac tgttgattca ccccggcgaa
    34621 ccgcgccccg gcgagcccgt ggtgcgcgtc acgcccgagt cggccggctg ggactacacc
    34681 ggcttcgaag tgcatcgcct cgccgacggc gaggagcgca gcttcgacgc cgacggccgt
    34741 gagctgtgcg cggtgatgct cggggggcgc gctgacgtgg gcgccggtga ccgctcgttc
    34801 gagggcgtcg gcgaccgggc gaacgtgttc gacggcctgc ccgccgcggt gcacgcgccc
    34861 gccgggcacg cgctgtcagt tcgtgcggta ggcggtgaag cggaagtggc actgtgctgg
    34921 tcgcccgccg agggcacgga cgcgctcgag cctgcactga tcgagcctgg cgacatccag
    34981 gtggagatcc gcggttcggg cagcatggag cgccgagttc atccgatcct tatggcggac
    35041 cgccaggccg agcgcttgct cgtggtggag gtgatcaccc cgggcggcaa ctactcgagc
    35101 tatccgccgc acaagcacga ccgcgacgac cccccgcgcg agacgctcct cgaggaggtg
    35161 tactaccacc gcatcgatcc ggcgcagggg ttcgggatgc agcgcgtcta caccgacgac
    35221 cgctcgctcg acgagaccat cacgttctcc gacggcgacg tcgtccttgt gccgcgcggc
    35281 taccacacgg tgtccgcgcc acccggctac gacgtctact acctaaacgt gatggtcggt
    35341 ccgcggcgcg agtggcactt cgtgaacgac cccgaccacg aatggctgtt cggccggtga
    35401 accgacccgc gggaacggtt cgcgccgcgg tgatgaagga gccgggacgc ctcgagctcg
    35461 agcgcttccc gttgccggat ccggaacccg gcgcggtgct gctgaagatg tcgtactccg
    35521 gcatctgcgg caccgacaag cacacgttcc gcggcgagac gctgcagtac gccggcacgc
    35581 cgcacgagcg caggctcgag tacccgctca tctgcggtca cgagaacgtc ggggtggtgg
    35641 cggcggtcgg cgggagcgag cccgtgctcg acagtgaggg cgtcccgctg cgcgacggcg
    35701 accgcgtggt gcccgccgcc aacgtcgcgt gcggcagctg ctggttctgc cgcaacgacc
    35761 agccgtacta catgtgcgag cacctcgacg actacggcaa cagcctcaac gccaagcggc
    35821 cgccgcacct cttcggcggc tgggccgagt acatgtacat cctccccggc acgccgctgt
    35881 tccgcgtgcc cgacgaactg cccgacgaga tcgccgtgct caccgaggtc atggccgtca
    35941 cgcacggatt cgagctcgcg cgacgcctct acaacaccaa cttcggggag agcgtggcgg
    36001 tgatcggcgt cggcccgctc ggcatgtgcc atctgatcaa ggcgaagctg ctcggctgcg
    36061 gcaagctgat cgcgatcgac cgcctgccct cccgcgtgga gatggcgggc cgcttcggcg
    36121 cgacgctcgg cctggacgcg tcacagctcg acgacgaaga gctgatcgcg cgcgtgcgcg
    36181 agcacaccgg cggacgcggc gccgacatcg tgctcgactg cagcggcgtg ccggagactt
    36241 tcgtcaccag cctgcacatg gcgcgcgtgg gcggaacggt ggtcgagtct ggcgcgttcg
    36301 tggacatggg gccggtcgcc atcaacccga acgccgacat ctgcacgaag aacgtgaacg
    36361 tgctcggcgt ggggggcgag accgccgagt cctaccagcc cgcgatggag ctgctagccc
    36421 gcaacatgga gcggctgccg ctgcacgagc tcgtgacgca ccggatggcg ctcgaggacg
    36481 cgcaggccgc gctcgagatc gctcagaacg acggcgcgat gaaggtggtg ctgtcgggcg
    36541 ctgtcgcgcc cgcgggcgac taggccggcg gcgccgtgga gtggcggacg acgagcgccg
    36601 ggttcatgcg gtgacgggcg ggcggagcgt cgtcgtcacc ctcgatgcgg tgcaggagca
    36661 gctcgaccgc gcgcgcggcc agctcgtccc gcggctgggt gaccgtggtg agcgcgatgc
    36721 gggagaggcc cgcgatctcg atcccgtcga agcccacgag cgagacgtcc gcgggcacct
    36781 ggatgccgtg ccgctcgagc agctccatca ggcgtatcgc cgtgtagtcg ttggcggcgg
    36841 cgaccgccgt ggggcgctcc gccgccgaga acagcgacat cagctcccgc tcgccgtctt
    36901 cgtcgtccgg gtcgaatacc agcgtccact cgctgcgggg cgagacgccg ccgcggagca
    36961 tcgcgcgctc gaacgcctcc gcacgcgcac ggcgcgttgt cacctccatg agcggatcga
    37021 ccacgtgcgc gatgcgcttg tgcccgaggg cgacgagatg ttcgaccgcc agctccacgc
    37081 cgaccatgtc gtcgacggcc acgcagtcgg tgtaatcgga ccagcagctg accatcacga
    37141 ccggcacgcg ctcgccgagc agctgcgaga gcacggcgcg gtcccccgtg aagtcgagca
    37201 gcgcgatgcc gtccacccgc tgctcgagaa gcgcctcgat gcgggacccc tcgcgctcgg
    37261 cgtgcccgcc cgtgttgcat acgagcgtgg tgtagccgcg tccggacgcc tgctgctcga
    37321 tccgcttcac gagctcggcg tcgaaggcgt tcgcgagatc gcccacgacc acgccgatca
    37381 ggttggtgcg ctggagcacc aggttgcgcg ccgtcgcgtt cggccggtac ccgagtgccg
    37441 cgaccgcttc gagcacccgc gcgcgtgtgc cggccgacac gctgcccgtg ccgcgcatca
    37501 cgttcgagac ggtggacttc gacaccccgc tcagcgcggc gacgtcgttg atcgtcggcc
    37561 gcctgaactg ggtcccatcc gagtgattca tgtcctcgcg cgtgcagtcg agtgccgcac
    37621 gatcagctcc ggcacgagcg tcacccgccg cggcggtgcg gagacgccgc ccgcgtggat
    37681 gcggtcgagc agcaactcga ggccgcgagt ggtgagctcg gtgcggggct gggacaccgt
    37741 ggtgaggccg atgcgctcgt gggccccaag ctcgataccg tcgaagccga cgaccgaaac
    37801 gtcttccggc acgcgtagtc cggccgcctc gagcgcgtcg atcagccgca ccgccatcac
    37861 gtcgtttcca gcggcgaagg ccgtgagccc gccgcctccg tccacgagct cgcgcaggct
    37921 gcgcagagcg gaagggctct cgtcctccca gtcgtcggcg ctcagcaatc gcggctgcag
    37981 cccatgagcg cgcatcgccc gctcgtagcc ggcgtggcgc gtggcgcgcg tcgtctcctc
    38041 cacgaggtgg ccgctcacgt gcgcgatcga gcggtggccg agcgcgtaga ggtgctccac
    38101 cgcggcggcc atgccggcgg cgtcgtcgac ggcgacgcag tcggcgcgtt cgtccgcgca
    38161 gctcacgacc accaccggca tttgcgccgc gcgcagctcg tcgagcaccg tgcggtcgcc
    38221 gctgaactga agcagcacca cgccgagcac cgcgcgctcg agcagcgtct ccacgcgctc
    38281 gcgctcgagc gcgagatcgc cgtcggtgtt gcacaccatc gtcgtatacg cgctctccga
    38341 ggcctcgagc tccatcagct tcacgagctc cgcgtagaac gtgttcgaga ggtcgccgac
    38401 cagcacgccg atcgtgttcg cccgccgccg caccaggtcg cgcgcgagcg cgttcgggcg
    38461 gtagccgagc gcctcgatcg attcgagcac tcgccgtcgc gtagtttcgg atacgcgccc
    38521 ggacccgcgg atcacgttcg acaccgtcga cttcgagaca ccgctgaggt tcgcgacgtc
    38581 gacgatcgtc gggcgctggg agccccagcg cggccgaccc ggtttcaggg tatgcccact
    38641 ttcatctgga acggtttacc aacggtcgtc gcgacgatac ggccaagcgg acgctcgcgt
    38701 caagcgggag cccactcttg ccgcacaatc caggcttaac gtggcgtatt cgggggacgt
    38761 gcttgacaag cccgtgcggt ttgtggaagg ttccacagca cggatggaac cttccatcga
    38821 atggcccttg gcatgaggct cgccctgctg acggacgccc tcgaggaccg gtcgcaggag
    38881 gatgccttcg cgtggtgtgc tgagcgcggg atcgccggcg tcgagctggg cgtgggcgga
    38941 tactcgccgg cgccgcacct cgatctcgcg gcgctgctcg cgagcgaggc cgagcgtgag
    39001 cgtctgacgg agaccctcga ggagcatggc ctcgagctcg tcgcgctgaa cgcctccggc
    39061 aacccgcttc accccgaccc ggcgatcgcg cgtgaccacg accgcgccct gcgcggtgcg
    39121 ctcgagctgg cggccgcgct cgatgtgcgg cgcatcgtgg cgatgagcgg ctgtccgggc
    39181 ggcccgggca gcggcggctg gcccgtgttc gccgggggcg cgtggctgcc cgacatggag
    39241 ggcctttggg accaccagtg ggagaacgcg atcgcgccct actggcgcga gctctccgct
    39301 tgggcgcacg aggccgcggc gggcgtcgac atctgcctcg agctgcaccc cggcacctcg
    39361 atctacaacg ctgcgtcgtt cgacctgctc tccgaggcaa ccggcgacaa cgtgaaggtg
    39421 aacctcgacc cgagccactt ctggtggcag ggcatcgacc cggtcacgac cgtccgcgcg
    39481 ctcggcgatc gcatcaggtt cgtgcacggc aaggacacga tcgtgtaccc cgaccgggtg
    39541 gcgctgcagg gggtgctcga cttccggtgg ccggccgacg cggacacgat gccgtggcac
    39601 ttctgtgcgg tcggccatgg gcgcccgctc gacgagtggc gcgagctgtt cgacgcgctg
    39661 agcgaggcgg gctacgacgg gccggtctcg atcgagcagg aggacccgac gctgagcccc
    39721 gagcagggga tcgaggcctc gctcgagggg cttcgcgcag tgatggaagc gaccgtgccg
    39781 ggacagcccg agcgctgagg ggggaccgcg ccgcgatgga cgtccagtac aagggagtct
    39841 cgaagacgtt cggctcgacg gaagcgctcg cgccgctcga cctcaccgtg ccggacggca
    39901 agttcctcgc gatgctcggc ccctccggct gcggaaagac caccgcgctg cggctgctcg
    39961 ccgggctgga gcagcccacg accggcagca tcttcatcgg cgaccgcgac gtgacgaggc
    40021 tcgagccgcg ccaccgcgac gtggcgatgg tgttccagag ctacgcgctc tacccgcaca
    40081 agagcgtggc cgacaacatc gcgtacccgc tgcgcctgcg ccgcggctca tccgccgagc
    40141 gtgacgagcg cgtgcgtaac gtcgccgagc tgctcggcat cagcgagctg ctcgaccgcg
    40201 cgccgcgcca gctctccggg ggccagcgcc agcgtgtcgc gctcgcgcgg gcgatcatcc
    40261 gccacccgca ggcgttcctc atggacgagc cgctgtcgaa cctcgacgcg cagcttcgcc
    40321 tgcagatgcg cgtggagatc aagcggctgc agcgcgagct aggcgtcacc acgctctacg
    40381 tgacgcacga ccagatcgag gcgatgacga tggccgatct gatcgccgta atgcgcgatg
    40441 gccacctgca gcagctcgcc accccggccg agctgtacgc gcacccgacc aatctgttcg
    40501 tcgcccgctt ctgcggctct cccccgatga acatcctcga cggcgaggtc accgacggcg
    40561 ccttccggca cgccggcgga tcgatctcgc tgcgcgacgc ccccgtccgc ggccccgtga
    40621 agctcggctt ccggccggaa cacgccgcgt tcgcggagcc cggcaccgcg gacgcgctcg
    40681 agggcgagat ctacgtcgtc gaaccgctcg ggaacgagac gctcgtgacc atgcgggtcg
    40741 atgggcagct gttgaacgtc cgcacgtcgg ccggcttcag ccggccggtc ggggagcggt
    40801 gcgcggtgcg gcctgtgccg gagcaggtcc acctgttcga cacggagtcg ggaggagcgc
    40861 tggcgcaaac cggcgcacga agcgcggaga gcgctggaca gccggcgaca gccgggttct
    40921 gaccgagggg ataggaggga aacgaatgtc ggatcaggag agggacgcag cccggccaga
    40981 agggagccgc ataacgcgga ggaaggtgat cgagcgcggc gcggtcggcg ccgccgggat
    41041 cttcggcgcg agcttcatcg ctgcgtgcgg cggcagcagc agtagcagca gcaccgccac
    41101 gaacgcgtcg aagggcgcga gcgggacgat cacgatcggc tcgttccagg acaacgcgat
    41161 ggcgccgttc cgggacacct tcatcaagag gttcacgaag gaaaccggta tcaacgtcaa
    41221 gtacaacgag acgagctacg acgcctggta ccagaacgcc aagaacgacg ggctcaacaa
    41281 gaccggcgcc tacgacatct acgtcatgga cgacaactgg gtgcccgagt tcgccgccgg
    41341 caacatcatc aagaacctcg acgagatggg gttcaaggtg aaccaggaca tcctcccgaa
    41401 gggcctgaac cagggctact ggccgccgaa ggagggcgct cgcctgaagg acttcgccaa
    41461 cgacgatccg gcgctctacg cgatcgtgat catcgacgac gtcgagcttc tctactacaa
    41521 caccgaccac ttcaaggacg cgcccaaaac ctgggacgag gtcttctccg ggatgaagtc
    41581 gaaggccaac ccgccgaagc tctacggctg gtccgcccgc ggcgtgaagg gcaacccgat
    41641 catgatgacc tacctgccgc tgctgaactc gtacggcggc gagttcgtga aggacgactg
    41701 gtcgccgggc ttcgccggtc cagagggcgt gggcgcgctc gagcggctga tgaccttcat
    41761 tccgtacatg ccgtcgggcg tcgccgagtt cgacaccgac caggagaccc agtcgatgct
    41821 ccagggcaag tgcatggcgc tcacggagta cacgggcctc gtgcatcggg tggacgacaa
    41881 gaagtcgtca caggt
//
LOCUS       DASVXS010000021         8262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_429481, whole genome shotgun sequence.
ACCESSION   DASVXS010000021 DASVXS010000000
VERSION     DASVXS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429481"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VF752_01205"
     CDS             complement(<1..302)
                     /locus_tag="VF752_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8854188.1"
                     /translation="MARPDDLLLPGTPRLLRAINERALLEDLRRRGPASRADLARASG
                     LSKPTVSQALANLESVGLVRAVGPATPARGRTALLYEFDPTAGFVVGVDIGRAWIR"
     gene            379..1344
                     /locus_tag="VF752_01210"
     CDS             379..1344
                     /locus_tag="VF752_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8854189.1"
                     /translation="MESPTPPPSGNSDFVLAIDFGGTKIALATADVEGHILEADRLDT
                     NAGDGAAQAIDRALGRAAALVAATAGQCLAVGAVSPGIVYPDRVWLAPNVPGWEGLAL
                     AQLLRESLGVARVELANDAKAAALAETRWGSLRGFDPAVLLNLGTGLSAGIVVGGDIL
                     EGANGAAGEIGYNLRGLADERGAADGRAPLEEFAGGRAIGERGSRLLGETFRAADVFA
                     HGDVRARLLIDEALAELSLHVANLAILLNPARIAVGGGLMSHADLVLAALERRISFAV
                     PFPPELVPAQFLHDGPLRGAVALALEAARGEAATDTDLLNARATS"
     gene            1389..2495
                     /locus_tag="VF752_01215"
     CDS             1389..2495
                     /locus_tag="VF752_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX8854190.1"
                     /translation="MRGTHRRLIIVALALLSAIGVAACGGGGDNGGGGGGGGGGKKIA
                     LLLPETKTTRYEAKDRPLFTAKVKQLCPDCQILYSNASQDAAKQQQQAEAAITNGANV
                     LVLDAVDVKSVGSIVKHANQKKIPVIAYDRLISNADIDYYVSFDNVKQGRMQAQSLLD
                     GLGSGAKGDPIVMVNGAPTDPSAGDYKKGAHEVLDKSGVKIAKEYDTPDWSPDKAQRQ
                     MEQSITALGKDGFDGVLSANDGMASGIIAAMKSAGIDPSKKPTTGGDSEAAAIQRILT
                     GEQYSTIYLAIKQQAETSAQLAVNAAQGKPPPTGIVNAKVNNGTEDVPSVLLSPIAVT
                     KDNINDTVIKDGFLTPDEICTGKYKSACQEAGIG"
     gene            2500..3324
                     /locus_tag="VF752_01220"
     CDS             2500..3324
                     /locus_tag="VF752_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8854191.1"
                     /translation="MTREVPGGAPTGAPASPTPLLEMRGIEKYYGPVHALTEVEFAVR
                     AGEVMGLVGDNGAGKSTLIKTISGVGPADAGEIRFAGELVRVRNPQHAAALGVAAVYQ
                     DLALCDNLDVVGNLFLNREERRWGRRGLLDEVAMEEQARRLLESLSIGGLPDVRREVA
                     TMSGGQRQSVAITRALLGEAKLVILDEPTAALGVVQTEQVLQLVRRLRDRGLGVVLVS
                     HNLADVFTVADRITVLRLGRNAGTFPAEPAFRQEVVAAITGVTGDLAPTGSEDADS"
     gene            3321..4544
                     /locus_tag="VF752_01225"
     CDS             3321..4544
                     /locus_tag="VF752_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854192.1"
                     /translation="MSALPSQVVAEPSAPSSELPGPRAALRKLGRGELGSLRVLIVLA
                     IIWTVFGIAHDRFLSAVNLTNLTLQIAAVGTISIGVVLVLLLGEIDLSVGAVSGLAGA
                     VMASLSVKAGWPAVPAISAALATGALIGLFQGTVATRLEIPTFVVTLAGLLAWQGALL
                     QVLGKTGTLNLTDPGIVNLANTFYGDAVGWILAVGSIAAICAAALLGRRRRAAAKLPL
                     EPLPALVLRVGAAAAAIVAAMAVLNSDRGVPLALLILVVLVVLFDTIAMRTGFGRHIY
                     AVGGNEEAARRSGIRVNRVRTIVFVLASTLAATGGVLEASRLLAANQSSGGGDLLLLA
                     IAGPVIAGVSLFGGRGRVWGALAGALVIGSISNGMDLLNFESSVKFMVTGGVLLAAVS
                     LDAIARMRRRAHGRL"
     gene            4600..5109
                     /locus_tag="VF752_01230"
     CDS             4600..5109
                     /locus_tag="VF752_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8854193.1"
                     /translation="MATPKEQIPSCAYVVGDDGISRWSDDSARAWIGLLETHKKLTRE
                     LDAELEAQHGLSLSGLEVLGRLAASDDTRMRLSSLAAETGLSLSRVSRIVDSLEDRAL
                     VERRPCPGDGRAINAHLTDAGLALAREAQSAHFAAVQERFFDQLSAEEVTTLSGVFAR
                     FTPPSCRGE"
     gene            5114..5326
                     /locus_tag="VF752_01235"
     CDS             5114..5326
                     /locus_tag="VF752_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854194.1"
                     /translation="MKVSTPLGDYPYEVVKVGFRDGRILVTGRMGEWETTMQVEPSDW
                     APPLALAGVTGLALLALGAFARGRRR"
     gene            5440..5970
                     /locus_tag="VF752_01240"
     CDS             5440..5970
                     /locus_tag="VF752_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8854195.1"
                     /translation="MTTQTQQGLPTGTWNADPIHSTVGFAVDYMAGTFQGSFSKFEAS
                     LADGSLRGSAEVSSIQVKDENLGAHLQAPDFFDVERHPTLTFEAREISRSGDDLTISG
                     EITIKGHTESIEIRGHVNDPFQDPWGNQRFGLQLEAKLDRTTFGLNWNNPLPSGEPAL
                     SDEVTLIADLQFVQGA"
     gene            5976..6533
                     /locus_tag="VF752_01245"
     CDS             5976..6533
                     /locus_tag="VF752_01245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX8854196.1"
                     /translation="MRILGISGSLRRDSHNTKLLRVAGRTAEAEGAEFVEFDGLKGVP
                     PYDEDDDVADAPAAVARLRGAIAGADAVFFATPEYNSSLPGQLKNAIDWASRPVATNP
                     LRNKPVAVVGASAGMFGAVWAQAELRKVLAATGARVAEGEVAVGHAQDRFDEDGNLND
                     ENLADQLREVVEALVAETKPAEIAA"
     gene            complement(6530..7030)
                     /locus_tag="VF752_01250"
     CDS             complement(6530..7030)
                     /locus_tag="VF752_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8854197.1"
                     /translation="MAPFTHSSGSEQQDAASAWFSHEAAFAALDEAPSHRQVVMIERS
                     AAEGSMAPLHRRDEDERYRVIEGAVTFYIGAEVITAETGDVVVAPRGAARTFRVESGA
                     ARWRVLTRVASLQRYEDFARAICPPVESPADGWPSPEEESAVAAIAEANGIELIGPPG
                     LLPEGS"
     gene            complement(7093..>8262)
                     /locus_tag="VF752_01255"
     CDS             complement(7093..>8262)
                     /locus_tag="VF752_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8854198.1"
                     /translation="GWVRLRRDAESPAGDEAAVIEIEAGALSGLFAAPKWLRDLGLTA
                     WLLFGVVAALTGAVVLLGLTKTIVLPVITAAIIASVMSPAVGWLEGRGVPRALGAVLV
                     FLVLILIGVAVFLGVVGGITSQSGKIGAHLHSATAKMQGWLEDLGVSDRTAAKANQDA
                     TGSASAGVKALLQGVRFGIQSLASLVVFLSFTLLSLFFLLKDAPTIRGWAQRHMGVPI
                     PVAQVITGRTTTALRGYFAGCTIVSAFTAVIVGIGALVLGVPLVGTIMVVTFLAGYVP
                     YLGAWTAGIFAVLIALGSQGTGAAVALALITLLGNGILQQMVQPVAFGATLGLHPLAV
                     LIVTIAGGSLFGTVGLILAAPLTSAVVQISSALAGARAEREEPLPAPGEPAEGSV"
BASE COUNT         1327 a         2878 c         2881 g         1176 t
ORIGIN      
        1 atgcggatcc aggcgcggcc gatgtccacc cccaccacga agccggcggt cggatcgaac
       61 tcatagagca gcgcggtccg gcctcgcgcg ggcgtggcgg ggccgaccgc acggacgaga
      121 cccacgctct cgaggttcgc cagcgcctgg gagacggtcg gcttcgatag tccgctcgcc
      181 cgcgcgaggt cggcgcgcga cgccggcccc ctccggcgca ggtcttcgag cagtgcgcgt
      241 tcgttgatcg cacgcagcag gcgcggcgtg cccggcagca gcaagtcgtc cggtcgagcc
      301 attcagccgt cgccttcgct tgatctcacg tattggcccc ttgactcgga cttagttagg
      361 aaagtatctt atctcgcgat ggagtcgccg actccaccgc ccagcggcaa cagcgacttc
      421 gtccttgcca tcgactttgg cggcacgaag atcgccttgg caaccgccga tgttgaggga
      481 cacatactcg aggccgaccg gctcgacacc aacgccgggg acggggctgc ccaggcaatc
      541 gaccgcgccc tggggcgagc ggcggcgctt gtcgccgcga ccgcggggca gtgcctcgcg
      601 gtgggcgcgg tcagcccggg catcgtctat cccgaccgcg tgtggctcgc gcccaacgtg
      661 ccgggctggg agggccttgc gctcgcgcag ctcttgcgcg agagcctcgg ggtcgcgcgg
      721 gtggagctcg ccaacgacgc aaaggctgct gccctcgccg agacgcgctg gggaagtctc
      781 cgtggcttcg atccagcggt tctcctcaac ctcggcacgg ggctgtccgc gggcatcgtc
      841 gtgggcggcg acatcctcga gggggcgaac ggggccgcgg gggagattgg ctacaacctc
      901 cgcggcctcg ccgacgagag gggtgcggcc gacggccgcg ccccgctcga ggagttcgcg
      961 ggcgggcggg caatcggcga gcgcggcagc cggcttctcg gcgagacatt ccgcgcggcc
     1021 gacgtcttcg ctcacggcga cgtcagggcg cggctgctca tcgacgaggc gctcgcggag
     1081 ctctcgctcc acgtcgccaa tctcgcgatc ctgctcaacc cggccaggat cgcggtgggg
     1141 ggcggcctca tgagccacgc cgatctggtg ctcgcggcgc tcgagcgccg gatcagcttc
     1201 gcggtgccgt tcccacccga gctggtccca gcccagttcc tgcacgacgg accgctgcgc
     1261 ggcgccgttg cgctggcgct cgaggccgcc cgcggcgagg cggcgaccga caccgatctt
     1321 ctgaatgcga gggccaccag ttgaaggcaa gtggacacct gcaaccccat caaaaggaga
     1381 gggacaccat gcggggcacg cataggcgat tgatcatcgt cgcgctcgcg ctgctgagcg
     1441 cgatcggagt tgccgcctgc ggcggcggag gcgacaacgg cggaggcggg ggtggtggcg
     1501 gcggcggcaa gaagatcgcg ctgctgctgc cggagaccaa gaccacgcgg tacgaggcca
     1561 aggaccggcc gctcttcacg gcgaaggtga agcagctctg ccccgactgc cagatcctgt
     1621 actcgaacgc gagccaggac gccgcgaagc agcaacagca ggccgaggcc gcgatcacta
     1681 atggagcgaa cgtgctcgtg ctcgacgcgg tcgacgtgaa gtcggtcgga agcatcgtca
     1741 agcacgcgaa ccagaagaag atcccggtga tcgcgtacga ccgcctgatc tcgaacgccg
     1801 acatcgacta ctacgtgtcg ttcgacaacg tcaagcaggg caggatgcag gcgcagtcgc
     1861 ttctcgacgg cctcggatcg ggcgccaagg gcgaccccat cgtgatggtc aacggcgctc
     1921 ccacggaccc gagcgcgggt gactacaaga agggcgcgca cgaggtgctc gacaagagcg
     1981 gagtgaagat cgccaaggag tacgacaccc cggactggag cccggacaag gctcagcgcc
     2041 agatggagca gtcgatcacc gcgctcggca aggacggatt cgacggcgtc ctttccgcga
     2101 atgacggcat ggcgagcggg atcatcgcgg ccatgaagag cgccggaatc gacccctcga
     2161 agaagcccac cacgggcggc gactcagagg ccgcggcgat tcagcgcatc ctcaccggtg
     2221 agcagtactc gacgatctac ctggcgatca aacagcaggc ggagacctcg gcccagctcg
     2281 ccgtgaacgc cgcgcagggc aagccgccgc cgaccggcat cgtcaacgcg aaggtgaaca
     2341 acggcaccga ggacgttccc tcggttctgc tgtcgccgat cgcggtgacc aaggacaaca
     2401 tcaacgacac ggtgatcaag gacggcttcc tcacgccgga cgagatctgc accggcaagt
     2461 acaagtccgc gtgccaggag gccgggatcg gctgatggga tgacccgcga ggtacccggc
     2521 ggcgccccga cgggggcgcc ggcctccccc actccgctgc tcgagatgcg cgggatcgag
     2581 aagtactacg ggcccgtgca cgcactcacg gaggtggagt tcgccgttcg tgccggcgag
     2641 gtgatggggc tcgtcggcga caacggcgcg ggcaagtcga ccttgatcaa gacgatctcg
     2701 ggggtgggac cggcggacgc gggcgagatc aggtttgcgg gagagctcgt ccgcgtccgc
     2761 aatccgcagc acgccgccgc gctcggcgtc gccgccgtct atcaagacct cgcgctgtgc
     2821 gacaacctgg acgtggtggg caacctcttc ctcaaccgcg aagagcgccg ctggggacgc
     2881 cgtggcctgc ttgacgaggt ggcgatggag gagcaggccc gcaggctgct ggagtccctc
     2941 tcgatcggcg ggctcccgga cgtgcgccgc gaggtcgcga cgatgtccgg cgggcagcgt
     3001 cagtcggtgg ccatcacgcg cgcgctgctc ggcgaggcga agctcgtgat cctcgacgag
     3061 ccgacggccg ccctcggggt cgtccagacc gagcaggtgc tccagctcgt gcggcggttg
     3121 cgcgaccgcg gtctcggcgt ggtgctcgtc tcgcacaacc tcgccgacgt cttcacggtc
     3181 gccgaccgga tcacggttct tcgcctgggt cggaacgcgg gcacgtttcc cgcggagccg
     3241 gcgttccggc aggaggtggt cgcggcgatc accggcgtga cgggcgacct cgcgcccacc
     3301 gggtcggagg acgccgactc atgagcgcgc tcccgtcgca ggttgtcgcc gagccgagcg
     3361 cgccgtcgtc cgagcttccg ggcccgcgcg ccgccctgcg caagctgggc cggggcgagc
     3421 tcggctcgct gcgggtgctg atcgtgctcg cgatcatctg gacggtcttc gggatcgcgc
     3481 acgatcgctt tctcagcgcg gtcaacctca ccaacctgac gctccagatc gccgccgtcg
     3541 ggacgatctc gatcggcgtc gtgctcgtgc tgctgctcgg tgagatcgac ctctccgtgg
     3601 gcgcggtcag cgggctggcg ggcgcggtca tggcaagcct ctcagtcaag gcggggtggc
     3661 cggccgtgcc ggccatctcc gccgccctcg cgaccggcgc gctgatcggc ctcttccagg
     3721 gcaccgtggc gacgcgcctc gagataccca cgttcgtcgt caccctggca gggctgctgg
     3781 cgtggcaggg cgcgctgctg caggtgctcg gcaagaccgg cacgctcaac ctcaccgacc
     3841 cgggcatcgt gaacctcgcc aacacgttct acggcgacgc cgtcggctgg atcctcgcgg
     3901 tcggctcgat cgccgccatc tgcgcagcgg cgctactcgg ccgccggcgg cgcgctgcgg
     3961 ccaagctccc gctcgagccg ctcccggcgc tcgtgctgcg cgtcggtgcc gccgccgccg
     4021 cgatcgtcgc ggccatggcg gtactcaact ccgatagagg cgtcccgctc gcgctgctga
     4081 tcctggtcgt gctcgtggtg ctgttcgaca ccatcgcgat gcggaccggc ttcgggcgcc
     4141 acatctacgc ggtagggggc aacgaggagg cggcgcggcg ctcaggtatc cgagtcaacc
     4201 gggtgcgcac catcgtcttc gtgctcgcgt cgacgctcgc cgcgacgggc ggtgtgctcg
     4261 aggcctcgag gcttcttgcc gccaaccaat cgtccggcgg cggagatctg ctgctgctgg
     4321 cgatcgcggg gccggtgatc gcgggcgtaa gcctcttcgg tgggcgaggg agggtatggg
     4381 gcgcgctcgc gggagcgctt gtgatcggct cgatctccaa cgggatggac ctgctcaatt
     4441 tcgagtcgtc ggtgaagttc atggtcaccg ggggcgtgct gctcgccgcc gtctcactcg
     4501 acgcgatcgc ccgcatgcgc cgccgcgcgc acggccgcct ctgagccccg gaaggctcgt
     4561 cgccgggcga ttcccgtcct atacttgcta gagcaagtta tggcaacacc caaggaacaa
     4621 attccctcct gcgcctacgt cgtcggggac gacggcatct cccgctggtc ggacgactcg
     4681 gcgcgcgcgt ggatcgggct gctcgagacc cacaagaagc tcacgcgcga gctcgacgcc
     4741 gagctcgagg cccagcacgg cctgagcctc agcgggctcg aggtgctcgg acgcctcgcc
     4801 gcaagcgacg acacgcgcat gcgactctcg tcgctagccg cggagacggg gctcagcctg
     4861 agccgcgtct cccgcatcgt cgactcgctc gaggaccgcg cgctcgtgga gcggcggccc
     4921 tgccctggcg acggccgggc catcaacgct catctcaccg atgcagggct cgcgcttgcg
     4981 cgcgaggccc agtcggcgca cttcgcggcg gtgcaggagc gcttcttcga ccagctgagc
     5041 gccgaagagg tcaccacgct gtccggcgtg ttcgcccgct tcacgccgcc gtcctgccgc
     5101 ggcgaatagc gccgtgaagg tctcgacgcc gctcggcgac tacccctacg aggtcgtgaa
     5161 ggtggggttc agagacggcc ggatcctcgt gaccgggcgc atgggcgaat gggagacgac
     5221 gatgcaggtg gagccttcgg attgggctcc accactggct ttggcgggcg tcacggggct
     5281 ggcgctgctg gcgctcggcg cgttcgcgcg cggacgccgg cgctgaatca cgggtttaac
     5341 ccgccaggga tggcagcaaa aaggttgtca acgcaagtat ccctgctaca cttgcttgcg
     5401 cacgcaaata aagcctgtct cctgaggagg aaaacagcag tgactacgca gacgcagcag
     5461 ggccttccca ccggaacctg gaacgccgac cccatccact ccacggtcgg cttcgcggtc
     5521 gactacatgg ccggtacgtt ccagggttcg ttctcgaagt tcgaggcttc gctcgccgac
     5581 ggctcgctcc gcggctccgc cgaggtctcg agcatccagg tgaaggatga gaacctcggg
     5641 gcgcaccttc aggcgccgga cttcttcgac gtcgagcgcc acccgacgct caccttcgag
     5701 gcacgtgaga tcagccgctc cggggacgac ctcacgatct cgggcgagat caccatcaag
     5761 ggccacaccg agagcatcga gatccgcggc cacgtgaacg atcccttcca ggacccgtgg
     5821 ggcaaccagc gcttcggtct ccagctcgag gcgaagctcg accgcaccac gttcggcctg
     5881 aactggaaca acccgctccc gagcggcgag ccggcgctct ccgacgaggt cacgctgatc
     5941 gcggacctgc agttcgtcca gggggcgtaa gccagatgcg gatcctcggg atcagcggca
     6001 gcctccgccg cgactcacac aacacgaagc tgctccgggt cgccggccgc acggcggagg
     6061 ccgagggcgc tgagttcgtg gagttcgacg gcctcaaggg cgtgccgccc tacgacgagg
     6121 atgacgacgt cgccgacgcc cccgccgcag tcgcgcgcct gcgcggtgcg atcgcggggg
     6181 cggacgccgt cttcttcgcg acgccggagt acaactcgtc cttgccgggg cagctgaaga
     6241 acgcgatcga ctgggcctcc cggccggtcg cgaccaaccc cctgcgcaac aagcccgtcg
     6301 ccgtggtcgg cgcgagcgcc ggaatgttcg gcgccgtctg ggcccaggcc gagctgcgca
     6361 aggtgctcgc cgcgaccggt gcgcgggtgg ccgagggcga agtcgccgta ggccatgccc
     6421 aggaccgctt cgacgaggac ggcaacctca acgacgagaa tctcgcggat cagctccgcg
     6481 aggtggtcga ggcgctcgtc gccgagacca agcccgccga aatcgcggcc tagctgcctt
     6541 cgggcagcag gccgggcggg ccaatcagct cgatcccgtt cgcctcggcg atcgccgcga
     6601 cggcgctctc ctcttcgggg gagggccagc cgtcggccgg cgactcgacg ggcgggcaaa
     6661 tcgcgcgggc gaagtcctcg tagcgctgca gcgaggcgac tcgcgtgagc acgcgccagc
     6721 gcgcggcgcc cgactccacc cggaacgtcc gcgccgctcc gcgcggcgcg accacgacgt
     6781 cgcctgtctc ggccgtgatc acttcggcgc cgatgtagaa ggtcacggct ccctcgatca
     6841 cccggtagcg ctcgtcctcg tcgcggcggt ggagcggggc catcgatccc tccgcggcgc
     6901 tgcgctcgat catcaccacc tggcggtggc tgggggcctc gtccagcgcg gcgaacgccg
     6961 cctcgtgcga aaaccaggcg ctcgccgcgt cctgctgctc gctgccggag gagtgcgtaa
     7021 acggggccac gctgaaaagt tcgcagccgg gcggcgcgcc gaacacgcgg gtaactcgcg
     7081 tctcgaagtg cgctaaaccg aaccttcagc tggctcgccg ggcgccggca gcggctcctc
     7141 gcgctcggcg cgggcgccgg cgagagccga cgagatctgc accacggccg acgtgagagg
     7201 ggcagcgagg atcaggccga cggtgccgaa gaggctgccg cccgcgatag tgacgatcaa
     7261 gaccgcgagc gggtgcaagc cgagcgtcgc accgaaggcg accggctgga ccatctgctg
     7321 caggatgccg ttgccgagca gcgtgatgag ggccagagcc actgccgcgc ccgtcccctg
     7381 actgccgagc gcgatcagca ccgcgaagat gccggccgtc cacgcgccga gatagggcac
     7441 atagccggcc agaaacgtca cgaccatgat cgtgcccacg agcgggacgc cgagcaccag
     7501 cgcccctatt cccacgatca ccgcggtgaa ggcgctgacg atcgtgcagc cggcgaagta
     7561 cccgcgcagc gcagtggtgg tgcgcccagt gatcacctgc gcgaccggaa tcgggacgcc
     7621 catgtggcgc tgcgcccagc cacggatggt cggggcgtcc ttgagcagga agaacaggct
     7681 cagcagcgtg aacgagagaa agaccacaag cgaggcgagg ctctggatac cgaacctgac
     7741 gccttgcagc agcgccttca cgccggcact tgccgagccc gtcgcgtcct ggttcgcttt
     7801 cgcggcggtt cggtcgctca cgcccaggtc ctcgagccag ccctgcatct tggccgtcgc
     7861 gctgtgaagg tgagcgccga tcttgccgga ctggctcgtg atcccgccga cgacgccgag
     7921 gaacaccgcg acgccgatga ggatcagcac caggaagaca agcaccgccc cgagcgctct
     7981 cgggacgccc cgaccctcga gccagcccac ggccggagac atgaccgacg cgatgatcgc
     8041 ggccgtgatc acgggcagga caatcgtctt ggtgaggccg aggagcacca cggcgccggt
     8101 aagcgccgcg acgacgccga acaagagcca cgctgtgaga cctaggtcgc gcagccattt
     8161 cggggccgcg aacaggcccg acagcgcgcc ggcctcgatc tcgatcaccg cggcctcgtc
     8221 tccagcgggc gactcggcat cacgacggag ccggacccac cc
//
LOCUS       DASVXS010000022         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_639283, whole genome shotgun sequence.
ACCESSION   DASVXS010000022 DASVXS010000000
VERSION     DASVXS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639283"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2469)
                     /locus_tag="VF752_01260"
     CDS             complement(<1..2469)
                     /locus_tag="VF752_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854199.1"
                     /translation="MRIRAIVVALLVVLVGGSAFAALKLTSGAGREEARSCPKGFVTR
                     EQAAARERHTEIALGHKPGKEDGDREAEPEERKQGECVPQKKPESYAELAAANSQLAS
                     RSTAPFKALKAGAYEHAVAQKAQLVKAGSGGVAGADGNWSPYGDGPLITDNTDYDQSN
                     GSTREGFGTVAGRTSDFAYDAPSGKLWAAASNGGIWESSNLGDSWHSIGDNLPTQVVS
                     SVKYSPAGGGTLLVLTGDGSFGFDSESGLGVYRSTDDGASWQKAGGIPDRALGFKLAV
                     DPTNPSIVYAATGLGLFRSTDAGASFTNVNLPTGDGVDPGQPDCTGDSSIEKGCYLAN
                     VVTDVVVQDSPHAPITGTGGAVMAAVGWRAGNKLNDDGTIESPNNGVYVSDDGTPGTF
                     TKVAPTSGSGDFPNQDKVGRTELGIANGPDQDHKIVYAVIEDAQKFRGGIPGIDAEGS
                     QTGAPYPSVFDAVYVTKDFGQTWTQLEDWMQLGTDPTSGSSLFAAGCAQLYCPGVQAW
                     YNEWIQPDPLQQTASGVPTRVMFGLEEVWSSKNPGSAQDNALTKFKVIGRYFGGDTCA
                     FLNSPLPVCPTNNPPTPSSTTHPDQHGSIVIPDGSGGETLVVGNDGGVYKQHVASGED
                     FDNTKWGRGANEGFHTLQPYDAEMAKDGTVYAGLQDNGEMKILADGRQYGVYGGDGFF
                     SAVDPDNSDVAYEEYTGGDISVTTDGGKNWTDIAPPFSAASFSNPFKMDPTDAKHLMA
                     PGREVFETVYGPDTTSELDGSKDWNQVFDLGTRKSPGTSASTSGSDDSDNKASALDLK
                     GDKAYVAYCGFCDVMTEGRPFESGI"
     gene            2611..3300
                     /locus_tag="VF752_01265"
     CDS             2611..3300
                     /locus_tag="VF752_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8854200.1"
                     /translation="MRLLLWDIDGTLIDSGGMGLDAFNEAFERIVGRPPTQWVPFAGR
                     TDLEIAADLAHVNGVNDGGEFVERFADELHDALVARVDVLRERGRALPGAGEALERLS
                     EEPGVIQSLLTGNVERNAPVKLSPFGLDRFLDFEVGAYGSDHHIRAELPAIARAKAER
                     KHAVEIPREDVVLIGDTPLDVAAARAAGARAVGVATGPYDEEALAQSGAHAVLPSLAE
                     LDAVLEAVLAP"
     gene            complement(3297..3512)
                     /locus_tag="VF752_01270"
     CDS             complement(3297..3512)
                     /locus_tag="VF752_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854201.1"
                     /translation="MPRLEAAYKRAADTHERAAATHDRAALQFDRVGKHELADRERER
                     ARVDRAGAVMDRERARLRRERTAARQS"
     gene            complement(3572..5026)
                     /locus_tag="VF752_01275"
     CDS             complement(3572..5026)
                     /locus_tag="VF752_01275"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8854202.1"
                     /translation="MNTVLEITEPQRHLIGGERRESSADEAIEVLNPATGEVLGRIPA
                     GTEADADAAVTAARAALPEWSARPVAERSAFLKQAARLLRDRVDELAELQTLENGKPL
                     ADSRGGVMAGIEAIDQYAELGPLHRGRSLNGAEGATDAMVHEPRGVVALLVPWNDPLA
                     VAAGQIAAALVAGNTVVFKPSEKTPLSAIRMVELMEPPAGVLNLLLGDSRAGRPLVAH
                     QGVNLVMHTGSVETGREIARVCAEQMKKALLELGGNDPLIVDDGVDPDWAAEQAASGA
                     FANAGQICTSVERIYVHEAVAEPFIDALVRKAEALAVGNGMDAGVEMGPLIDEGQREV
                     VHRHVSEAVGAGARVLTGGEPAEGPGTFYPPTVLVDVSDNMALMRDETFGPVAPVMVV
                     PSFEAALEAANSGRYGLAASVLTPSEEHARRACRELEAGTVKVNAVWGGAPGGAAHPR
                     KSSGSGFGYGPELLDELTCTKVVHMETAGPLLSA"
     gene            5073..>5356
                     /locus_tag="VF752_01280"
     CDS             5073..>5356
                     /locus_tag="VF752_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854203.1"
                     /translation="MQRYYRIDPADRDPERLLDPEHQESEPWSGTVYGRCDKCGGSGR
                     TQFDCDSCRSGPDADCPVCHGEVTYEDDCPACKGTGEIDDSSRDGVSVFPT"
BASE COUNT          716 a         1893 c         1855 g          892 t
ORIGIN      
        1 gattccgctc tcgaacggac ggccctcggt catgacgtcg cagaagccgc agtaggccac
       61 gtacgccttg tcgcccttga ggtcgagcgc cgacgccttg ttgtcggagt cgtccgagcc
      121 gctcgtgctc gcgctcgtgc cagggctctt gcgcgtgccg agatcgaaca cctggttcca
      181 gtccttcgag ccgtcgagct cagaggtcgt gtcagggccg tagacggtct cgaacacctc
      241 gcgccccggt gccatcaggt gcttggcgtc ggtcgggtcc atcttgaacg ggttcgagaa
      301 gctcgcggcg ctgaacggcg gagcgatgtc ggtccagttc ttgcctccgt ccgtggtcac
      361 gctgatgtcg ccgcccgtgt actcctcgta ggcgacgtcc gagttgtccg ggtcgaccgc
      421 gctgaagaag ccgtcaccgc cgtacacccc gtactgcctg ccgtcggcga ggatcttcat
      481 ttcgccgttg tcctgcaggc cggcgtacac ggtgccgtcc ttggccatct cggcgtcgta
      541 gggctggagc gtgtggaagc cctcgttcgc gccgcggccc cacttggtgt tgtcgaagtc
      601 ctcgccggac gcgacgtgct gcttgtacac gccgccgtcg ttacccacga ccagcgtctc
      661 gcccccggac ccgtccggga tcacgatcga gccgtgctgg tccgggtgcg tcgtgctgga
      721 cggcgtgggc gggttgttgg tcgggcagac gggcagcggg ctgttcagga acgcgcaggt
      781 gtcgccgccg aagtagcgcc cgatcacctt gaacttcgtg agcgcgttgt cctgcgcgga
      841 accggggttc ttgctcgacc agacctcctc gaggccgaac atcacccgcg tcgggacgcc
      901 gctcgcagtc tgctggagcg ggtccggctg gatccactcg ttgtaccagg cctgcacgcc
      961 cgggcagtag agctgagcac agcccgccgc gaacagcgag gaccccgatg tcggatcggt
     1021 gccgagctgc atccagtcct cgagctgcgt ccaggtctgg ccgaagtcct tcgtcacgta
     1081 gaccgcgtcg aacacggacg ggtagggggc accggtctgg ctgccctcgg cgtcgatgcc
     1141 gggaatgccg ccgcggaact tctgcgcgtc ctcgatcacg gcgtagacga tcttgtggtc
     1201 ctggtcgggc ccgttcgcga tgccgagctc cgtgcggccg accttgtcct ggttgggaaa
     1261 gtcgccgctg ccgctcgtcg gcgcgacctt ggtgaaggtg ccgggcgtgc cgtcgtcgga
     1321 cacgtagacg ccgttgttcg gcgactcgat cgtgccgtcg tcgttcagct tgttgccagc
     1381 gcgccagccg acggccgcca tcaccgcgcc gcccgtcccg gtgatcgggg cgtgcgggct
     1441 gtcctgcacc accacgtcgg tcacgacgtt cgcgaggtag cagcccttct cgatgctcga
     1501 gtcgccggtg cagtcgggct ggcccgggtc gacaccgtcg cccgtcggca agttcacgtt
     1561 ggtgaagctc gcgccagcgt cggtggagcg gaagaggccg aggccggtgg ccgcgtagac
     1621 gatgctcggg ttcgtcgggt cgaccgccag cttgaagccg agcgcgcggt caggaatgcc
     1681 gcccgccttc tgccaactgg cgccgtcgtc ggtcgaccgg tacacgccca ggccgctctc
     1741 cgagtcgaag ccgaaggagc cgtcgccggt gagcacgagc agcgtgcccc ctccggcggg
     1801 cgagtacttg accgaggaga cgacctgcgt gggcaggttg tcgccgatcg agtgccagct
     1861 gtcgccgagg ttgctcgact cccagatgcc gccgttcgag gcggcggccc acagcttgcc
     1921 gctgggcgcg tcgtaggcga agtcgctcgt gcgtccggcc accgtgccga agccctcgcg
     1981 cgttgagccg ttcgactggt cgtagtcggt gttgtcggtg atcagcggcc cgtcgccgta
     2041 gggcgaccag ttgccatccg ccccggcgac gccgccgctg cccgccttca ccagctgcgc
     2101 cttctgggcg accgcgtgct cgtaggcgcc cgccttcagc gccttgaagg gagcggtcga
     2161 gcggctggcg agctggctgt tcgccgccgc gagctcggcg tagctctcag gcttcttctg
     2221 aggcacgcac tcgccctgct tgcgctcctc gggctcggcc tcacggtcgc catcttcctt
     2281 gcctggtttg tggccgaggg cgatctcggt gtggcgctcg cgcgcggcgg cctgctcacg
     2341 cgtgacgaag cccttcgggc acgagcgtgc ctcctcgcgg cctgccccgc tcgtcagctt
     2401 cagcgcagcg aaggcgctcc cgccgacaag caccaccagc aacgcgacga cgatcgccct
     2461 gatgcgcatt cctgcctcct tggtctgccc acacagacca gcccctgatt tccatgcccg
     2521 ttgtgctgcg ccgctccctg cggcgagcgc aggatacccc tatggggtcg ccgttagtct
     2581 cactaggggg gtcctagact cggcggacgg atgcgcctgc tgctctggga catagacggg
     2641 acgctgatcg actcgggcgg catggggctc gacgcgttca acgaggcgtt cgagcggatc
     2701 gttggccgtc cgcccacgca gtgggtccca ttcgcggggc ggacggacct cgagatcgcg
     2761 gccgacctcg cacacgtgaa cggcgtgaac gacggcggcg agttcgtgga gcgcttcgcg
     2821 gacgagctcc acgacgcgct cgtggctcgc gtggacgtgc tgcgcgagcg cggccgcgcg
     2881 ctgccgggag cgggggaggc gctcgagcgc ctctccgagg agcccggcgt gattcagtcg
     2941 ctgctcaccg gcaacgtgga gcgcaatgcg cccgtgaagc tgagcccgtt cgggctcgat
     3001 cgcttcctcg acttcgaggt gggcgcctac ggctccgacc accacatcag ggctgagctg
     3061 cccgcgatcg cgcgcgcgaa ggcggagcgc aagcacgccg tcgagatacc gcgcgaggac
     3121 gtggtgctga tcggcgacac gccgctcgac gtcgcggcgg cgcgagcggc cggcgcgcgc
     3181 gccgtcgggg tggcgaccgg accctacgac gaggaggcgt tagcgcagtc gggcgcgcat
     3241 gcggtgctgc cgtcgctcgc ggagcttgac gctgtgctcg aggccgtgct cgcgccctaa
     3301 ctctggcgcg cagcggtccg ttcgcggcgc agccgcgccc gttcacggtc catcaccgca
     3361 ccagcgcgat ccacgcgtgc gcgctcacgc tcgcgatcag ccaactcatg cttgccgacg
     3421 cgatcaaatt gcagcgccgc ccggtcatgg gttgcggcgg cccgctcatg ggtgtccgca
     3481 gcacgcttat aggcagcctc aaggcgaggc accctcccag cgtacgctca gtgggcgcga
     3541 cttgctagtt gcttcgccag acacacctgc gtcatgcgct caacaaggga ccggccgtct
     3601 ccatgtgcac gaccttcgtg cacgtgagct cgtcgagcag ctccggcccg tagccgaagc
     3661 cgctcccgct cgacttgcgt ggatgagcag cgccgccggg tgcgcctccc cagaccgcgt
     3721 tcaccttcac ggtccccgcc tcgagctcgc ggcacgcgcg cctggcgtgc tcctccgagg
     3781 gcgtgagcac cgatgcggcc agcccgtagc gcccggagtt cgccgcctcg agcgcggcct
     3841 cgaagctggg caccaccatc acgggcgcga cggggccgaa cgtctcgtcg cgcataaggg
     3901 ccatgttgtc gctcacgtcc accaaaacgg tcggtgggta gaacgtgccg ggtccctcgg
     3961 caggctctcc gcccgtgagc acgcgcgcgc cggcgccgac cgcctcggac acgtgccggt
     4021 gcaccacctc gcgctggccc tcgtcgatca gcggacccat ctccacgccc gcgtccatgc
     4081 cgttgccgac ggcgagcgcc tcggccttcc gcacgagcgc gtcgatgaac ggctcggcga
     4141 cggcctcgtg cacgtagatg cgctcgaccg acgtgcagat ctgcccggca ttcgcgaacg
     4201 ctcccgaggc cgcctgctcg gccgcccagt ccgggtcgac gccgtcgtcg acgatcagcg
     4261 gatcattccc accgagttcg agcagcgcct tcttcatctg ctccgcgcac acgcgagcta
     4321 tctcgcggcc ggtttcgacc gagcccgtgt gcatcaccag gttgacgccc tggtgcgcga
     4381 ccagcggacg gcccgcgcgc gagtcgccga gcaacaggtt cagtacgcct gcgggcggtt
     4441 ccatcagctc gaccatgcgg atagcggaga gcggcgtctt ctccgacggc ttgaagacga
     4501 cggtgttgcc ggcgacaagc gcggcggcga tctgtcccgc agcgaccgcc agcgggtcgt
     4561 tccagggcac gagcagtgcc actacgccgc gcggctcgtg gaccatcgcg tcggtcgcgc
     4621 cttcggcgcc gttcaggctg cggccgcgat gcagcggccc gagttccgcg tactggtcga
     4681 tcgcctcgat cccggccatc acgccgccgc gcgaatcggc gagcggcttg ccgttctcga
     4741 gggtctggag ctccgcgagc tcgtcgactc tgtcccgcag caggcgtgcg gcctgcttga
     4801 ggaatgcgga acgctcggcg accgggcgcg ccgaccactc cggaagcgcg gctcgcgcgg
     4861 cggtgaccgc ggcgtctgcg tccgcttcgg tacccgcagg gatgcggccg agtacctcgc
     4921 cggtcgcggg gttgagcacc tcgatcgctt cgtcggcgct cgactcgcgg cgttcgccgc
     4981 cgatcagatg gcgctgcggc tcggtgatct cgaggacggt gttcatgggc ggcggtttac
     5041 ccgcgccgag cggcggggaa ccactggcgg ccatgcagcg ctactaccgc atcgaccccg
     5101 cggaccgcga cccggagcgc ctgctcgacc cggagcacca ggagtcggag ccctggagcg
     5161 gcactgtgta cgggcgctgc gacaagtgcg gcggctcggg caggacccag ttcgactgtg
     5221 attcgtgccg ctctggaccg gacgccgact gccccgtgtg tcacggcgag gtgacgtacg
     5281 aggatgactg cccggcgtgc aagggcaccg gcgagatcga cgactcgagc cgcgacggag
     5341 tgagcgtgtt cccaac
//
LOCUS       DASVXS010000023         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1349677, whole genome shotgun sequence.
ACCESSION   DASVXS010000023 DASVXS010000000
VERSION     DASVXS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349677"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..816)
                     /locus_tag="VF752_01285"
     CDS             complement(100..816)
                     /locus_tag="VF752_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854204.1"
                     /translation="MTRDRFTSLVAGAALIPLTALAAGCGSSGSSTAATRGAAPSPAH
                     ASSARAATLRVANSRLGKILVDSQGRTLYLFKKDSLNKSACSGQCVTFWQPLRTSGKP
                     VASSGVTASLLGTIKRSDGKSQVTYNGHPLYTFAMDHKAGDTNGEGVTAFNARWFAVS
                     RSGKQVSPKAQTHTRGSTTAPAPKAATPAAPPKSAAPAAPKPPPAAKPPAARNGIPQN
                     GGGDGDSDNNGGPDDGDGGV"
     gene            1329..>2426
                     /locus_tag="VF752_01290"
     CDS             1329..>2426
                     /locus_tag="VF752_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8854205.1"
                     /translation="MVTKEPRELLLGRRTEIDVLDRLLADVAGGHSGVLVLRGEPGVG
                     KTALLKHVISSASAFRVVRAVGIESEMELPFAVVQQVCAPLMDRIERLPGPQRDALCV
                     AFGLTAGDPPDRFLVGLAVLSLLSTVAEEQPLLCVVDDSQWIDHASTDALAFVARRLL
                     AEPLAFVFASRQPAGSFTGLPELVVQGLREGDACALLDSVIGWPLDERVRDRIVAETR
                     GNPLALLELPRGLTAAELAGGFVLPQAHALPSRIEESFRRRIEGLPAETQRLLLVAAA
                     DPVGDPRLMWLAADGLGIGASAADAAEAEGLLEVGAVVTFRHPLVRSAVYRAAVPDEK
                     RTVHRALAQATDPEVDPDRRTWHLAQATVGFD"
BASE COUNT          304 a          789 c          903 g          430 t
ORIGIN      
        1 gcgggcatca ctccgggctt ggcagtcaca ggtgctcctt cgggtggttg tgttgttgac
       61 cgggcaggcg ctctcgcggt acatcgccct cgggtccgct cagacgccgc cgtcgccgtc
      121 gtccgggccg ccgttgttgt cggagtcgcc gtcaccgcca ccgttctgcg gaatgccgtt
      181 gcgggctgcg ggcggcttcg cggcgggcgg aggcttcgga gctgccgggg cggccgactt
      241 cggcggggct gcgggggtgg cggccttcgg agccggcgcc gttgtgctgc cgcgcgtgtg
      301 cgtctgcgcc ttaggcgaaa cctgcttgcc ggaccgggaa accgcgaacc agcgcgcatt
      361 gaacgcggtc acaccctcgc cgttcgtgtc gccggccttg tgatccatcg cgaacgtgta
      421 gagcgggtgc ccgttgtagg tcacctgcga cttgccgtcc gagcgcttga tcgttccgag
      481 cagcgaggcg gtcacgccac tgctcgcgac cggctttccg ctcgtgcgca gcggctgcca
      541 gaacgtcacg cattggccgg agcaggcgct cttgttcagt gagtccttct tgaacaggta
      601 gagggtgcgc ccctgcgagt cgacgaggat ttttccgagg cgactgttcg cgacgcgcag
      661 cgtcgccgcg cgcgctgaag aggcgtgggc gggagatggc gccgcgcccc tcgtcgccgc
      721 ggtgctggag ccactgctgc cgcagccggc ggccagcgcg gtgagtggta tcagcgccgc
      781 gccggcgaca agggatgtga atctgtcgcg agtcatctcg ctcctttggt gctggggacg
      841 gcccagatgt tccctttgga gctcggcgat ttcgtcgtct agccgcggtc tctttggcct
      901 gcggcgagcg ctgtatcggc accgcggact acggcagtcg cggcatcgaa aagatcggct
      961 tggcgagtga cgacaagcgg ggccgcaggc gtggacgatt gtcgcgtgag accgccgagg
     1021 cgtctcgagt gagccaagtg ggacagccga atatcgtgag cacgcgaatc tccgcgtccg
     1081 cccaagtcgt cttcgagttc ctcgaacgcc cgcggtccga cggctgtcct gctgcgtcgc
     1141 cgctgcgagc cccgggccgc gcggtccggg cggctacgag tcccgcttcg taggcgaaag
     1201 cgacgagccg cgcgcgcgtg tgcgcgtgta acttgagcat cgttgagcat cgctcccgtg
     1261 gcgagtgcga tcagcgggat cgcaaacgtg tagtccaggt cgctcaggcg cggtagggtc
     1321 gctctggcat ggtcacgaag gagccgaggg agctcctcct cggccgtcgg acggagatcg
     1381 atgtgctgga ccggctgctg gcagatgtgg ccggcggtca cagcggtgtg ctcgttctgc
     1441 gcggcgagcc gggtgtgggc aagacggctc tcctgaagca cgtgatctcg tcggcttccg
     1501 cgttccgagt cgtccgcgcg gtcggcatcg agtcggagat ggagctcccg ttcgcggtgg
     1561 tgcagcaggt gtgcgcgccg ctgatggacc ggatcgaacg gttgcccggc cctcagcgcg
     1621 acgcgctttg cgtggcgttc ggcctgactg ccggcgatcc gcccgaccgg ttcctggtcg
     1681 ggctggcggt gctgagcctg ctgtccaccg tcgccgagga gcaaccgctg ctgtgcgtgg
     1741 tcgacgactc gcagtggatc gatcacgcct cgaccgacgc gcttgcgttc gtcgcgcgcc
     1801 ggctgctggc ggagccgctc gcattcgtat tcgcgtcgcg ccagccggca ggatcattca
     1861 ctgggctgcc cgagctggtg gtccaagggc tgcgcgaggg cgacgcgtgc gcgctgctcg
     1921 attcggtgat cggttggccg ctggacgagc gcgtccgcga ccggatcgtc gccgagacgc
     1981 gcggcaaccc gctggccctc ctcgagctgc cgcgaggatt gacggcggcc gagctggccg
     2041 gcggctttgt gctgccccag gcgcacgcgc tcccctcgcg gatcgaggag agcttccggc
     2101 gacggatcga gggccttccg gccgagaccc agcggctgct gctggtcgcg gcggcggacc
     2161 cggtcggcga tccgaggctg atgtggctcg cggccgatgg acttgggatc ggcgcctcgg
     2221 cggcagacgc agccgaggct gaggggttgc tcgaggtcgg cgccgtggtg acctttcgcc
     2281 atccgctcgt gcgctcggcc gtctaccggg ccgcggtgcc cgacgagaag cgaacggtac
     2341 atcgtgcgtt ggcccaggca acggaccccg aggtcgatcc cgatcgccgc acctggcacc
     2401 ttgcgcaggc aaccgtaggg ttcgac
//
LOCUS       DASVXS010000024         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_73590, whole genome shotgun sequence.
ACCESSION   DASVXS010000024 DASVXS010000000
VERSION     DASVXS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73590"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF752_01295"
     CDS             <1..214
                     /locus_tag="VF752_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854206.1"
                     /translation="IDTLERVAPDEYLVLLCFGVRGSVSGAPAEGVPLAHLVRVRDGR
                     VDRIRAFFKIREAREATVLTQHFDAS"
     gene            447..953
                     /locus_tag="VF752_01300"
     CDS             447..953
                     /locus_tag="VF752_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX8854207.1"
                     /translation="MTWPREKLRRNVTDGRPGTPLTLQLSVVDASSCKAIQRATVEIW
                     HCDASGAYSHFNAGGNFLRGAQKTDAKGVATFQTIYPGWYQGRTVHIHVKVHLGGSVV
                     HTGQLYFSDGLTDAVYRRSPYKSRGPRDTRNSADAIYRNGGKRSMLSVRKSGSGYVGT
                     IAMGVQRA"
     gene            complement(950..>1642)
                     /locus_tag="VF752_01305"
     CDS             complement(950..>1642)
                     /locus_tag="VF752_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX8854208.1"
                     /translation="GEVKVASGRKPDLGGRATERIPGLRGIPKLAEAFAIVPVVKKNL
                     PEAQLPMEDFRALLGMAGASLAGQLVRRSGPRTVAREAAVALISLAPAMLVLRGGDIA
                     SYHGVEHKAIGAYETGEETVDATKEHERCGSNLVAPLLASTVIGNVAARRAGLRGPKA
                     DAVVGLGSIAAAVEVFAWSERHADSGLARALRKPGYEIQRLLGTREPSAEQIEVGEAA
                     LAEILRVEQRAA"
BASE COUNT          242 a          607 c          561 g          232 t
ORIGIN      
        1 gatcgacacg ctcgagcgag tcgcgccgga cgagtatctg gtgctgttgt gcttcggagt
       61 gcggggaagc gtgagcggag cgcccgcgga gggcgtcccg ctcgctcacc tcgtgcgtgt
      121 gcgggacggc agagtcgacc gcatccgggc gttcttcaag atccgcgagg cgcgcgaggc
      181 gaccgttctt acccaacact tcgacgcctc ttagacggcc ctgggttggc ggccgtaggt
      241 tcgctcgatg cctgacgaac ccatcacccg gcgcggctcc ctgatcaagg ccggcggcct
      301 gctcgcggcg accctcggcg cggccggctg cgcctccgaa tcgtcgaagg aatcgacctc
      361 caccgctgag gcatcgacgg gccccgtcgg cgtggccagc ggcgcggtga agtgcgtgct
      421 cgctcccgac cagaccgagg gcccgtatta cgtggccccg ggagaagcta cggcgcaacg
      481 tcaccgacgg gcggccgggc acgccgctca cgctgcagct gagcgtggtg gacgcctcca
      541 gctgcaaggc gatccagcgt gcgaccgtgg agatctggca ctgcgacgcg agcggcgcct
      601 actcccactt caacgctggc ggcaacttcc tgcgaggagc gcagaagacg gacgcgaagg
      661 gcgtcgccac cttccagacg atctaccccg gctggtacca gggccgcacc gtgcacatcc
      721 acgtgaaggt gcacctcggc ggaagcgtgg tccacactgg ccagctctac ttctcagacg
      781 ggctgaccga cgcggtgtac cggcgctcgc cgtacaagag ccgcggaccg cgtgacacgc
      841 gcaactccgc cgacgcgatc taccgcaacg gcggcaagcg ctccatgctc tcggtgcgca
      901 agagcggatc gggctacgtg ggcacgatcg cgatgggcgt gcagcgcgcc taggcggccc
      961 gctgctccac ccgcaagatc tccgcgagcg ccgcctcgcc cacctcgatc tgctcggcgc
     1021 tcggctcgcg cgtgccgagc aggcgctgga tctcgtagcc aggcttgcgc agggccctcg
     1081 cgagcccgct gtcggcgtgg cgctccgacc acgcgaaaac ctcgaccgcc gcggcgatcg
     1141 agccgaggcc caccacagcg tcggccttcg gtccacgcag tccggcgcgc cgcgccgcca
     1201 cgttgccgat cacggtcgac gcgagcagcg gcgccaccag gttcgacccg cagcgctcgt
     1261 gctccttggt cgcgtcgacg gtttcctcgc cagtctcgta ggcgccgatc gccttgtgct
     1321 cgacgccgtg gtaggacgca atgtcgccgc cgcgaagcac gagcatcgcg ggcgcgagcg
     1381 agatcagcgc gacggccgcc tcgcgggcga ccgtgcgcgg cccgctgcga cgcacgagct
     1441 gcccggcgag cgacgcgccc gccatcccga gcagcgcgcg gaagtcctcc atcggcagct
     1501 gcgcttcggg caggttcttc ttcacgaccg gcacgatcgc gaacgcctcc gcgagcttcg
     1561 gaatgccgcg cagtcccggg atccgctcgg tcgccctgcc gccgaggtcg ggcttgcgtc
     1621 ccgaggcgac cttgacctcg cc
//
LOCUS       DASVXS010000025         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_215335, whole genome shotgun sequence.
ACCESSION   DASVXS010000025 DASVXS010000000
VERSION     DASVXS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215335"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF752_01310"
     CDS             <1..210
                     /locus_tag="VF752_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854209.1"
                     /translation="AVLAAVSYVVWWGLDKALGRALWAQVVSVGGGISAGFVAYAAVV
                     WALGIDEARQIWNLFAGRFKRRQNT"
     gene            245..2068
                     /gene="lepA"
                     /locus_tag="VF752_01315"
     CDS             245..2068
                     /gene="lepA"
                     /locus_tag="VF752_01315"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX8854210.1"
                     /translation="MAAPAQNIRNFSIIAHIDHGKSTLADRILEVTGAVDPRNHRPQL
                     LDSMELERERGITIKAQAVRVEYTARDGETYRLHLIDTPGHVDFTYEVSRSLAACNGA
                     LLVVDASQGVEAQTLANTYMAIDAGLELIPALNKIDLPGAEPERVAGEISELLGEPAD
                     DVLRVSAKTGEGVEELLEAIVHRIPPPDGDADAPPRALIFDSEFDQYRGVIAYVRVVD
                     GELKKNDAIKAMQTGTEADIDEIGFFGPQMTTVDSLRAGEVGYLITGIKDVSKLRVGD
                     TITTRANAAAEALPGYREVKPMVFCGLFPVDTDDFEDLRDALEKLALNDAALTYEPET
                     SQALGFGFRCGFLGLLHMDIVRERLEREYDLALLATTPNVEYDVVLTNGEEVRVHSPS
                     DMPDRARIAEIREPYIRATILCPKEHVGAVMELCQERRGTHVGMSFLSTTRVQLQYDL
                     PLAEIVLDFFDQLKSRTKGYASLDYELIGERPSDLVKLDILLAGDMVDALSMVVHKDK
                     AYGAGRAMTERLRKQIPRQQFEVAIQASIGSKIIARESVKPVRKDVIAKCYGGDITRK
                     RKLLEKQKEGKKRMKQVGRIEVPQEAFLSVLQIGDGDGRAS"
     gene            2098..2171
                     /locus_tag="VF752_01320"
     tRNA            2098..2171
                     /locus_tag="VF752_01320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2132..2134,aa:Pro,seq:cgg)
     gene            complement(2319..2777)
                     /locus_tag="VF752_01325"
     CDS             complement(2319..2777)
                     /locus_tag="VF752_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854211.1"
                     /translation="MATRWDGDNRGLGIGDALQLVSGASELVAAFRQPAWVAEQPEIH
                     LLPHVEAWCERDRRLRLIDAYTDDTNAYILDCEWQRAPASVGEARAAVFSLIGSFAES
                     ATYVRQRRVASNGGGSTMKLQFEVGTGELAPDTRFVPHGHVVVINLAGVL"
     gene            2872..>3142
                     /locus_tag="VF752_01330"
     CDS             2872..>3142
                     /locus_tag="VF752_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854212.1"
                     /translation="MLGVAPAHWARRKPGIVALGAATARALVLGAPLIYVVALAITIA
                     SWGLPLARDQLFMWLGLGMAAFSLSAWRTWGGMLLEWLPFFGLLVA"
BASE COUNT          526 a         1050 c         1067 g          499 t
ORIGIN      
        1 gccgtgctgg ccgcggtgag ctacgtcgtg tggtggggac tcgacaaggc gctcggccgc
       61 gcgctgtggg cgcaggtggt gtcggtgggc ggcggcatca gcgcgggatt cgtcgcctac
      121 gcggccgtgg tctgggcgct cggaatcgac gaggcacgac agatctggaa cctcttcgca
      181 ggacgcttca agcggcgcca gaacacctga gcagagcgct tttgctcacc tatcctcgac
      241 tgtcgtggct gcgcctgccc agaacatccg caacttctcg atcatcgccc acatcgacca
      301 cgggaagtcg accctggccg accgcatcct cgaagtcacg ggcgctgttg acccgcgcaa
      361 ccaccggccg cagctgctcg actcgatgga gctcgagcgc gagcgcggca tcacgatcaa
      421 ggcgcaggcg gtgcgcgtgg agtacacggc gcgcgacggc gagacctacc ggctgcacct
      481 gatcgacacc cccggtcacg tggacttcac ctacgaggtg tcgcgctcgc tcgccgcgtg
      541 caacggcgcg ctgctcgtgg tggatgcgtc acagggcgta gaggcgcaga cgctcgccaa
      601 cacctacatg gcgatcgacg caggcctcga gctgatcccg gcgctgaaca agatcgacct
      661 gcccggcgcc gagccggagc gagtcgctgg cgagatctcc gagctgctcg gcgagccggc
      721 cgacgacgtc ctgcgggtgt cggccaagac gggggagggg gtggaggagc tgctcgaggc
      781 gatcgtccac cgcatcccgc cgcccgacgg cgacgcggac gcgccgccgc gcgcgctcat
      841 cttcgactcg gagttcgacc agtaccgcgg tgtgatcgct tacgtgcgcg tggtcgacgg
      901 agaactgaag aagaacgacg cgatcaaggc gatgcagacg ggcaccgagg ccgacatcga
      961 cgagatcggc ttcttcggcc cgcagatgac gacggtcgac tcgctgcggg caggcgaggt
     1021 cggctatctc atcaccggca tcaaggacgt gtcgaagctg cgcgtgggcg acacgatcac
     1081 gacacgcgcg aacgccgcgg cagaggctct gcccggctac cgcgaggtga agccgatggt
     1141 gttctgcggc ctcttcccgg tcgacaccga cgacttcgag gacctgcgcg acgcgctcga
     1201 gaagctggct ctgaacgacg ccgccctcac ctacgagccc gagacgagcc aggcgctcgg
     1261 cttcggcttc cgctgcgggt tcctgggcct gctgcacatg gacatcgtgc gcgagcgcct
     1321 cgagcgcgag tacgacctcg ccctgctcgc gaccacgccg aacgtggagt acgacgtcgt
     1381 cctgacgaac ggtgaagagg tgcgcgtgca ttcgccgagc gacatgccgg accgggccag
     1441 gatcgccgag atccgcgagc cctacatccg agcgacgatc ctctgcccga aggagcacgt
     1501 cggcgccgtg atggagctgt gccaggagcg gcgcggcaca cacgtgggga tgagcttcct
     1561 gtccacgacg cgcgtacagc tgcagtacga cctgccgctc gccgagatcg tgctcgactt
     1621 cttcgaccag ctgaagtcac gcaccaaggg gtatgcatcg ctcgattacg agctgatcgg
     1681 cgagcgcccc tcggacctcg tgaagctcga catcctgctc gccggcgaca tggtcgacgc
     1741 gctgtcgatg gtggtgcaca aggacaaggc ctatggcgca gggcgcgcga tgaccgagcg
     1801 gctgcggaag cagatcccgc gccagcagtt cgaggtggcg atccaggcct cgatcggctc
     1861 gaagatcatc gctcgcgaga gcgtgaagcc cgtgcgcaag gacgtgatcg ccaagtgcta
     1921 cggcggcgac atcacgcgca agcgcaagct gctcgagaag cagaaggaag gcaagaagcg
     1981 catgaagcag gtcggccgga tcgaggtgcc gcaagaggcc ttcttgtcgg tgctgcagat
     2041 cggcgacggc gacggccgcg cctcctgatc gggcgctaga ctcgcgattc cacgcttcgg
     2101 ggcgtggcgc agcctggtag cgcgcaccgt tcgggtcggt gaggtccccg gttcaaatcc
     2161 gggcgccccg atttggctgg cgtctgagcg cccctggctt tgtcgccgct gctggggccg
     2221 tgcccagcac tgtccccagc agcgctccgc gccaggaacg ctcagccgca agcccaatcg
     2281 gggcgccttc ggcccccaat ttctggcgac cgctttgcct agagcacacc cgccagattg
     2341 atcacgacca cgtggccatg cggaacgaat ctcgtgtcgg gagcaagctc gccggtgccg
     2401 acctcaaact gcagtttcat cgtcgagcca ccgccgttgc ttgcgacgcg gcgctggcga
     2461 acataggtcg cactttcggc gaatgatccg atgagtgaga acacagccgc ccttgcctcc
     2521 ccgacgctgg caggcgcgcg ttgccattcg cagtcgagga tgtacgcatt cgtgtcgtcg
     2581 gtgtaggcgt cgatcaaccg aagtctgcgg tctcgctcac accacgcttc gacatgcggg
     2641 aggagatgga tttccggctg ctcggctacc caggcgggct gcctgaatgc tgcgacgagc
     2701 tcgctggcgc ccgaaacgag ctggagggca tcaccgatgc cgagcccacg gttgtcgccg
     2761 tcccaccggg tcgccacaga cgcacgctac accagggact ggcctcgacc agcgctctcc
     2821 tgagcggcgt ttccgcacgc gggctccgac ccgctgtagg aagatctgct catgctggga
     2881 gtcgctcccg cccactgggc ccggcggaag cccgggatcg tggcactcgg ggcagccacc
     2941 gcgcgagcac tcgtcctcgg tgcaccgctc atctacgtcg tagcgctcgc gatcacgatc
     3001 gccagctggg gccttccgct cgcgcgggac cagctcttca tgtggctggg actggggatg
     3061 gccgcgttca gcttgagcgc gtggcgcacc tggggcggga tgctgctcga gtggctgccg
     3121 tttttcggtc tgctcgtcgc gt
//
LOCUS       DASVXS010000026         6971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_147873, whole genome shotgun sequence.
ACCESSION   DASVXS010000026 DASVXS010000000
VERSION     DASVXS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147873"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF752_01335"
     CDS             <1..762
                     /locus_tag="VF752_01335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8854213.1"
                     /translation="LSTLLLLAVAALGGGEAVWLCVPAALVLARAARTPGQAYASAAV
                     AAIASVVPALAGHGALPSLPLVAIVVGSSTALLVSMRSRLEHERDAQRATSLRDPLTG
                     LANRRAFDERLAYEAARHTRQSRTFAVVALDLDGFKSVNDRFGHQAGDDVLRDVAVAL
                     DRAVREQDTVARMGGDEFCVLAPETDRAGAEHLADRAEQAVRNATRGLAVLSASVGVG
                     MFPEDGREAAQVLEAADAAALYAKRQSRRARQRAA"
     gene            812..1846
                     /locus_tag="VF752_01340"
     CDS             812..1846
                     /locus_tag="VF752_01340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8854214.1"
                     /translation="MDPRFLAPALDVPVRPGARPSFSVMIAAYQAAATIGEALDSVFA
                     QTEPPQEVIVVDDGSTDDLDGALAPYAGRIRLIRQENAGVAAARNTALRAATGEFVVI
                     LDADDVFAPGRLEALGDLAVARPDLDILTTDAYMTLGGRVVRRYYDQGLNFEVDSQRE
                     RLLRGNFVYPLAAVRRERALVAGAFDEHMPPGEDWDMWLRLVLDGSRVGLVNEPLAEY
                     RLSESSISADARRMARGCMTVLEKHRADPRLTDRERAVLEAAYADYARELEREDARAA
                     LAACEQHARRKSLRIVLGRGHRPATRAKALLAAVAPRAARRRMESSMEQYFSGGFTVS
                     KYPSGRSRNR"
     gene            1893..3194
                     /locus_tag="VF752_01345"
     CDS             1893..3194
                     /locus_tag="VF752_01345"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6411 family protein"
                     /protein_id="HEX8854215.1"
                     /translation="MDQRFVAPAPDAPITRGTRPSFTVVIAAYQAAATIGDALDSVFE
                     QTEPAHEVIVVDDGSTDDLAAALAPYLDRIDLIRKRNGGVASARNRALERATGDFLVI
                     LDADDRFLPGRLEALGDLAVERPDLDILTTDAYMTLDGAVVRRYYEDGLRFEASDQLG
                     RLVRGNFVYPLAAVRRERLLAAGGFDEKLLTASDWEIWLRLALDGSRVGLVDEPLAEY
                     RLSTSSISSDGARMARACMAVIDKHRGDARLTETQRAAMGQARRGYELERDREEARAA
                     LRGDAPHARRKLLRIAARPGHGTRTRVKAALAALVPHTAHRRLRGSAAQYLTGGYTVA
                     DEYTPFGRGMPVMDTEEVLMLIAAIVGVCILLLILGFLVPRLSRGPQRGVDKALGLGS
                     RSASKAPGPLGRWFSKPFRTSMKAADKSAATGRRGRRKLPM"
     gene            3328..3789
                     /locus_tag="VF752_01350"
     CDS             3328..3789
                     /locus_tag="VF752_01350"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8854216.1"
                     /translation="MSVCRQQAQLDVPLELVWALVSDPDRYREWWPRFRDAQCPEGLR
                     EGCEFPAVMAGPLGTLEDHRLVVDDFEDCRELNIRCVGTGTYTHWTLTESRGGTFIDA
                     EFGMDPTTVPYRVFDVLAGKRYFRRWLKESLTALSSAAEGGVAARPESAID"
     gene            3833..4036
                     /locus_tag="VF752_01355"
     CDS             3833..4036
                     /locus_tag="VF752_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854217.1"
                     /translation="MERNTTTPTIEEEDRSLDWACAGRTPTSEDRRAAVNPAANPGPR
                     GNQEIDEHDCSRSRERFLAVLGG"
     gene            complement(4033..4971)
                     /locus_tag="VF752_01360"
     CDS             complement(4033..4971)
                     /locus_tag="VF752_01360"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX8854218.1"
                     /translation="MSALDAARAWADARTFHHSEFPPERLRDERSGSVSVCLPAREEA
                     ATIGATVEQLIELRKLGAIDQVVVIDAASADGTADIAAHLGAEVHQQSELLPELGNVQ
                     GKGDALWRALTVLDGDVICFLDADSLDFGAHFATGMVGPLVCGPPEIQLVKGAYRRPL
                     KLGEVALPEGGGRVTELMARPLLNLFYPELAGVRQPLAGEIAARRSLFERLPFTSGYG
                     IEIAMLIDAYREVGLDGLAQVDLEVRQNRHQPLGDLSAMAYAVLRAVALRLEREGRLR
                     GLETEGLLLPRDGGLEEAAPALVERPPLTSFLAGRS"
     gene            complement(4978..5385)
                     /locus_tag="VF752_01365"
     CDS             complement(4978..5385)
                     /locus_tag="VF752_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854219.1"
                     /translation="MAKKILALVSEPISADALKSAVGKHEVERAEVLVVAPALNSKIK
                     FWTSDTDEAIGRAEAVQEETVERLDEEGIDAAGDTGEADPLLALQDALQTYPADEIVL
                     FTHARGERNWQEEGIVDEAKARFDRPVSHLVVE"
     gene            complement(5415..5795)
                     /locus_tag="VF752_01370"
     CDS             complement(5415..5795)
                     /locus_tag="VF752_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX8854220.1"
                     /translation="MSAAEVVGALAIGFAAGVASGLLGIGGGALFVPGLAVVLGLSQV
                     NAEATSLLAIIPVSLVGAWRQRRYGNVRLQDGVLVGVLSPIGVVAGVVAAHALPNRVL
                     EVMFAVVLLYFAFTLARLALRHEG"
     gene            complement(5795..6151)
                     /locus_tag="VF752_01375"
     CDS             complement(5795..6151)
                     /locus_tag="VF752_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8854221.1"
                     /translation="MSRRVVRLALVATVAGAFSGLFGVGGGTVIVPLLIIWFAYEPRV
                     ATGTSLLAIAVIATFAASTHGAYGSVDVAKGVLIGLPAIAGVVAGTWLQQRVPQRVIA
                     GIFALVLVASAISLVV"
     gene            6171..>6971
                     /gene="otsB"
                     /locus_tag="VF752_01380"
     CDS             6171..>6971
                     /gene="otsB"
                     /locus_tag="VF752_01380"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX8854222.1"
                     /translation="MPAPTAETLADALRPLTADPARAAVFCDIDGTLAPIVQRAGDAK
                     VPQRTSQLLGRIAKRYGCVACISGRAAAEARRLVGVGGIMYAGTHGAEILRPGATRPE
                     VPEELASWTGRVREFARERDNARLRTLRVRIEDKGPIVAFHWRGAPDEEAAHARVEQL
                     AGEAEAAGFATHWGRKVLEVRPPVPISKGRAVFELVRGSRAETAMFGGDDATDLDGWD
                     ALDELVEDGTLKAAVRVGVRSDEGPEAIVARADLLVDGVPGYQEVLTVL"
BASE COUNT         1039 a         2451 c         2491 g          990 t
ORIGIN      
        1 ctatcgacgc tgctgctgct cgccgtggcc gcgctcgggg gaggcgaggc cgtgtggctc
       61 tgcgtgcccg ctgcgctcgt gctcgcccgt gccgcaagaa cgccgggcca ggcgtacgcg
      121 tcggccgccg tggcggcgat cgcctccgtc gttccagcgc ttgcgggtca cggcgcgctc
      181 ccgtcgctgc cgctcgtggc gatagtcgtc ggctcgagca ccgcgctgct cgtctcgatg
      241 cgcagccgcc tcgagcatga gcgcgatgcg caacgcgcca cgtcgctgcg cgatcccctc
      301 accggcctcg ccaatcggcg ggcgttcgac gagcgcctgg cctacgaggc cgcgcgccac
      361 acgcgccagt cgcgcacgtt cgccgtcgtc gcgctcgacc tcgacgggtt caagtccgtg
      421 aacgaccgct tcgggcacca ggcgggcgac gacgtgctgc gcgatgtcgc cgtcgcgctc
      481 gaccgcgccg tgcgcgagca ggacaccgtg gcgcgcatgg gcggcgacga gttctgcgtg
      541 ctcgctcccg agaccgaccg agcgggtgcc gaacacctcg ccgatcgcgc agagcaggct
      601 gttcggaatg cgacgcgcgg cctcgcggtg ctcagcgcga gcgtcggcgt ggggatgttc
      661 cccgaggacg gtcgcgaggc agcgcaggtg ctcgaggccg cggatgctgc ggcgctctac
      721 gcgaagcgcc agagcaggcg cgcgcggcag cgggcggcct gagcgcttac cggtgtggcg
      781 agttcgccgg cgccgtgtta ccgatgtcgg gatggatccc cggttcctcg caccggccct
      841 ggacgtgccg gtcaggccgg gcgcccggcc gagcttcagc gtcatgatcg ccgcgtacca
      901 ggcggcggca acgattggcg aggcgctcga ctcggtgttc gcccagacgg agccgccgca
      961 ggaggtgatc gtggtcgacg acggctcgac cgacgacctc gacggcgctc ttgcgccgta
     1021 cgccggccgc attcgcctga tccggcagga gaacgcgggc gtcgcggccg cccgcaacac
     1081 ggcgctgcga gccgcgaccg gcgagttcgt cgtgattctc gacgcagacg acgtgttcgc
     1141 gccaggccgg ctcgaagccc tcggcgatct ggcagtcgcg cgacccgacc tggacatcct
     1201 caccaccgac gcctacatga cgctcggcgg gcgggtggtg cggcgctact acgaccaggg
     1261 gctcaacttc gaggtcgata gccagcgcga gcgactgctc cgaggcaact tcgtctatcc
     1321 gctcgcggcc gtgaggcgtg agcgcgcgct ggtggcgggc gccttcgacg agcacatgcc
     1381 gccgggcgag gactgggaca tgtggctgcg gttggtgctc gatggctcgc gcgtgggcct
     1441 cgtgaacgag cccctcgccg agtaccggct atccgagtcg agcatcagcg cggatgcacg
     1501 ccgcatggca cgcggatgca tgacggtgct ggaaaagcac cgcgcggacc cgcgcctgac
     1561 cgaccgggaa cgcgccgtgc tcgaggccgc gtacgctgac tacgcgcgcg agctggagcg
     1621 agaggatgcg cgagccgctc tggccgcctg cgagcagcac gcgcgccgga agtcgctccg
     1681 aatcgtgctt gggcgagggc accggccggc cacgcgggcg aaggcgctgc tcgctgcggt
     1741 ggcgccgcga gcggcgcggc gacgtatgga gtcgtcgatg gagcagtact tctcgggcgg
     1801 cttcaccgtt tctaaatatc cgagcgggcg cagtcgcaac cggtagcggt tcgtggcgtg
     1861 gtgcgtgggc gtcgtgctac gcctttgcgt gcatggatca gcgcttcgtc gcgccggcgc
     1921 cggatgcgcc aatcacgcgg ggcaccaggc cgagcttcac cgtcgtcata gcggcgtacc
     1981 aggctgcggc cacgatcggc gacgcgctcg actcggtgtt cgagcagacc gagcccgctc
     2041 acgaggtgat cgtcgtcgat gacggctcga ccgacgacct ggcggcggcg ctcgctccgt
     2101 acctcgaccg catcgacctg atccgcaagc gcaacggcgg cgtcgcgagt gcgcgcaacc
     2161 gcgcgctcga gcgcgcgacc ggcgacttcc tcgtgatcct cgatgccgac gaccgattcc
     2221 tacccggccg cttggaggct ctcggcgacc tcgcggtgga gcggccggac ctcgacatcc
     2281 tcacgaccga cgcatacatg acgctcgacg gggcggtcgt gcgccgctac tacgaggacg
     2341 ggctccgctt cgaggcgagc gatcagctgg ggcggttggt ccggggcaac ttcgtgtatc
     2401 cgctggccgc agtgcggcgg gagcgcctcc tggctgccgg cggcttcgac gagaagctgt
     2461 tgaccgcatc ggactgggag atctggctgc gactcgcgct cgacggctca cgcgtggggc
     2521 ttgtcgacga gcccctcgcg gagtacaggc tgtcgacgtc gagcatcagc tccgacggcg
     2581 cgcggatggc ccgcgcgtgc atggcggtga tcgacaagca ccgcggcgac gcgcgtctca
     2641 ccgagacgca acgtgcggcg atgggccagg cgcgccgtgg ctacgagctc gagcgcgatc
     2701 gtgaggaggc ccgtgccgcg ctgcgcggcg acgcgccgca cgcgcggcgc aagctgctcc
     2761 gaatcgcagc ccgccccggc cacgggacgc gaacccgggt gaaagcggcg ctcgcggcgc
     2821 tcgtgccgca cacggcgcat cgcaggcttc gcgggtcagc ggcccagtac ctcaccggcg
     2881 gttacaccgt cgcggacgag tacaccccgt tcgggcgggg tatgcccgtg atggacacgg
     2941 aggaggtcct gatgctcatc gcagcgatag tcggtgtctg catcctgttg ctcatcctcg
     3001 gcttcttggt gccgaggctg tcgcgcgggc cgcagagagg agtagacaag gcgctcgggc
     3061 tcggaagccg cagcgcgtcg aaggcgcccg ggccgctcgg gcgctggttc agcaagccgt
     3121 tccgcacgtc gatgaaggcc gccgacaaga gtgcggcgac aggccggcgc ggccgccgca
     3181 agcttcctat gtgaccgtct gcccgccccc ggcacggggc gggcctcgcc taacggtcgt
     3241 gcgagatgct catagaacag agcaggggtt actcgtgcga acgtaaccca ggttgcgcgc
     3301 cgccgcaagc gcgcacagaa ttcatgcatg tccgtgtgcc gccagcaggc ccagctcgac
     3361 gtcccgctag agctggtgtg ggcactcgtg agcgaccccg accgctaccg cgagtggtgg
     3421 ccgcgcttcc gggacgcgca gtgtccggag gggctccgcg agggctgtga gttcccagcg
     3481 gtcatggcgg gtccattggg cacgctcgag gatcaccggc tggtggtgga tgacttcgag
     3541 gattgccgcg agctgaacat ccgctgcgtc gggacgggca cctacacgca ctggaccctc
     3601 acggagtcgc gcggaggcac gttcatcgat gccgagttcg ggatggaccc gacgaccgtc
     3661 ccataccggg tgttcgacgt gctcgccgga aagcgctact tccgccgctg gctcaaggag
     3721 tcgctcaccg ctctcagcag cgccgcggag ggcggagtag cggcgcgccc agagtcggca
     3781 atcgattgac cggtcaggtt tagctcggcc tcttcctgga atgcgaatgg gtatggagag
     3841 gaacacgact acaccgacaa tcgaagagga agaccggtcc ctggactggg cctgcgcggg
     3901 gcgcacgccc acgagcgagg atcgccgcgc cgccgtgaac ccggcggcaa atcccggtcc
     3961 gcgcggcaac caggaaatcg acgagcacga ctgctcccgc agccgcgagc gtttcctcgc
     4021 ggtactcggg ggctaactcc ggccggccag aaaagaggtg agcggcgggc gctccacaag
     4081 cgccggcgcc gcctcctcga ggccgccgtc gcggggaagc aggaggccct ccgtctcgag
     4141 accgcggagg cgcccttcgc gctccagccg aagtgcgacc gcgcgcagca ccgcgtacgc
     4201 catcgcggag aggtcgccga gcggctgatg gcggttctgc cgcacctcga ggtccacctg
     4261 cgcgagtccg tcgagcccca cctctcggta ggcgtcgatc agcatggcga tttcgatgcc
     4321 gtacccgctc gtgaacggca gacgctcgaa caacgaccgc cgcgccgcga tctcaccggc
     4381 gagcggctgg cgcacgcccg cgagctcggg atagaaaagg ttgaggagcg gccgcgccat
     4441 cagctcggtc acgcgcccgc cgccctctgg cagcgcgacc tcgccgagct tcagcggacg
     4501 ccggtaggcg cccttcacga gctggatctc cggcgggccg cacacgagcg ggcccaccat
     4561 gccggtcgcg aagtgcgcgc cgaagtcgag cgaatcggcg tcgagaaagc agatgacgtc
     4621 cccgtcgagc acggttagag cccgccacaa cgcgtcgccc ttcccctgca cgttgccgag
     4681 ctccggtagc agctcggact gctggtgcac ctccgcgccg agatgggcgg cgatgtccgc
     4741 cgtgccgtca gccgaggccg cgtcgatcac gacaacctgg tcgatcgctc cgagcttgcg
     4801 cagctcaatc agctgctcca cggtcgcgcc gatcgtcgcc gcctcctcgc gcgcggggag
     4861 gcacaccgag accgagccgc tgcgctcgtc gcgcagccgc tccggcggga actcggagtg
     4921 gtggaacgtg cgcgcgtccg cccatgcgcg ggcggcgtcg agcgcgctca cgtgcggcta
     4981 ctccacaacg aggtggctga ccgggcggtc gaagcgggcc ttcgcctcgt cgacgatgcc
     5041 ctcctcctgc cagttgcgct cgccgcgcgc gtgggtgaac agcacgatct catccgccgg
     5101 ataggtttgc agggcgtcct ggagcgcgag cagcgggtcc gcctcgccgg tgtctccggc
     5161 ggcgtcgatg ccttcctcgt cgagtcgctc gactgtctcc tcttgcaccg cctcggcgcg
     5221 gccgatcgcc tcgtcggtgt cggacgtcca gaatttgatc ttgctgttga gcgccggcgc
     5281 taccacgagc acctccgccc gctcgacctc gtgcttgccg actgcgctct tcagcgcgtc
     5341 ggccgagatc ggctctgaca ccagtgcaag gatcttctta gccatgggcg ggtcttaccc
     5401 acagcgcccg gggatcaccc ctcgtggcgt agcgccagac gcgcgagcgt gaacgcgaaa
     5461 tagagcagga ccacggcgaa catcacctcg agcacgcggt tcggcagcgc gtgagccgcg
     5521 accacaccgg cgaccacgcc gatgggcgac agcacgccga cgagcacgcc gtcctggagc
     5581 ctcacattgc cgtatcggcg ctggcgccaa gctcccacga gcgatacggg gatgatcgcc
     5641 agcagcgagg tcgcctctgc gttcacctgg ctgagaccga gcacgaccgc gagcccgggt
     5701 acgaacagcg ctccgccgcc gatcccgagc agcccgctcg cgaccccggc ggcgaagccg
     5761 atcgcgagcg cgccgacgac ctctgcagca ctcactagac gaccagcgag atcgccgagg
     5821 ccaccagcac cagcgcgaag atccccgcga tcactcgctg ggggacgcgc tgctggagcc
     5881 atgtcccggc cacgacgccc gcgatggccg gcaggccgat gaggacgccc ttcgccacgt
     5941 cgaccgagcc gtaggcgccg tgcgtgctcg cagcgaatgt ggcgatcacg gcgattgcga
     6001 gcagggatgt tccggtcgcg acccggggct cgtaggcgaa ccagatgatc agcagtggga
     6061 cgatgaccgt gcccccaccc acgccgaaca gcccgctgaa tgccccggcc accgtcgcca
     6121 cgagcgcgag ccgcacgacc ctccgtgaca tggcgggcat actaaggctc gtgcccgctc
     6181 caaccgccga aacgctcgca gacgcgctcc gcccgctgac cgcggatccg gcgcgagcag
     6241 cggtcttctg cgacatcgac ggcacgctcg ccccgatcgt gcagagggcc ggcgacgcca
     6301 aggtgcccca gcgcacgtcg cagctcctcg ggcgcatcgc gaagcgctac ggctgcgtcg
     6361 cgtgcatctc cgggcgcgcg gcggcggagg cccggcggct ggtcggcgtg gggggcatca
     6421 tgtacgccgg cacccacggc gcggagatcc tgcggccggg cgcgacgcgt cccgaggttc
     6481 ccgaggagct cgcttcctgg acgggccgag tgcgggaatt cgcgcgtgag cgcgacaacg
     6541 ctcgtctgcg cacgctgcgc gtgaggatcg aggacaaggg cccgatcgtc gctttccact
     6601 ggcgcggcgc cccggacgag gaggccgccc acgcacgcgt ggagcagctc gcgggcgagg
     6661 ccgaagcggc ggggttcgcc acgcattggg ggcgcaaggt gctggaggtg cgccctccgg
     6721 tcccgatctc gaaggggcgt gccgtatttg agctcgtgcg cggctcgcgc gcggagaccg
     6781 cgatgttcgg cggcgacgac gcgaccgacc tcgacggctg ggacgcgctc gacgagcttg
     6841 tcgaggacgg cacgctcaag gccgccgtgc gcgtaggtgt gcgctcggac gagggcccgg
     6901 aggcgatcgt tgcgcgcgcc gatctcttgg tcgacggcgt gcccgggtat caggaagtac
     6961 tcaccgtgct t
//
LOCUS       DASVXS010000027         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1352247, whole genome shotgun sequence.
ACCESSION   DASVXS010000027 DASVXS010000000
VERSION     DASVXS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352247"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VF752_01385"
     CDS             <1..708
                     /locus_tag="VF752_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX8854223.1"
                     /translation="AAAVGALTYAPMSNLALWLILAGVPLIFGLWAQTRVKKTFNRYS
                     DVPTANGVTGAQVGEAVVRSSGLPDVSVRPVQGQLTDHYDPRTRTLNLSEGVYQSHTV
                     AAAGVAAHEAGHAIQDAQHYRPMRIRQTLVPAAQIGQSLWFLPVFIGLIIGATGLVTI
                     GLILFAAVVLFQLVTLPVEFDASKRALVALEHQAILAPQELNGAREVLRAAALTYVAG
                     FVAALGQLLYFFIASRR"
     gene            877..1338
                     /locus_tag="VF752_01390"
     CDS             877..1338
                     /locus_tag="VF752_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8854224.1"
                     /translation="MSTTTTRAQKRAAIAAFNQSFDDFVDAIRRARGRAERDRSEGLS
                     LSQFKLLQPLLTFEPRPVGELAAAACVSAPSATRMIDGLERDGLVTREHSPEDRRVVA
                     VRLTDEGRQAVEAKRDYVEGKRRAIYESLSPNEREQAQRLLTHIAQVIDEL"
     gene            1328..2746
                     /locus_tag="VF752_01395"
     CDS             1328..2746
                     /locus_tag="VF752_01395"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854225.1"
                     /translation="MSSDAGVGRQHYNVTLGVLAISALAYALSQTMVAPALPDIQHTL
                     GTSTTTVTWVLTAYLLSASVATPIIGRLGDIFGKERVLVLTLLVFGFGSLVAALSHSI
                     GILILGRAIQGTGGAVFPLAFGIIRDEFPPGKVASGIGLISATFGIGGGAGLVISGLL
                     VDHLNYEWIFWLGLIFVAIAIVATHRYVPESPVKAKAKIDWLGAALMSVGLVALLIGV
                     SEGNSWGWGSDRVLGLFAASIVILAIWVWVETRVEEPLVDMRIMRERAVFTTNLTALF
                     VGFGMFGSFILIPQFVQAPLSTGYGFGASVTTAGLFLLPSALVMLPAGPIAGTLGERY
                     GSKLPLLAGSVLAALAYLFLAVAHDQRWEIYAATTLLGFGIGFAFAAMANLIVEAVPQ
                     DQTGVATGMNTIARTIGGALGAQLSATILAADHLASGYPAESGYTGAFAMSAVGVAAA
                     FFVGLAIPARRHPAAALAHEGA"
     gene            2756..3043
                     /locus_tag="VF752_01400"
     CDS             2756..3043
                     /locus_tag="VF752_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854226.1"
                     /translation="MAKLPDARWWSLRRAQNRKPATYTCPFCGGYLASMSEHMLIAPE
                     GDSSRRRHAHMACVLAARKSGRLPTRDEWLRSERGERPSFVARLLGRLQRQ"
     gene            complement(3120..>3958)
                     /locus_tag="VF752_01405"
     CDS             complement(3120..>3958)
                     /locus_tag="VF752_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8854227.1"
                     /translation="PDGYADYVRMATERSLERCGVDSFDLLLLHNPDRIGYTSEAVWK
                     ALESVRGVGLARQLGVAPGPANGFTLDLIGCFERFGSSIDWAMIILNPMEPWPGELAL
                     PAASEHGVELITRVVDYGGLFWDDVRDGHQFAQFDHRKFRPEGWVEAGRERLERMRPI
                     AERHGLTPMQLACEWNLSQPPVRVVAPTLIQEAGEGARPIEEKRAELASLPAEAVLSP
                     DEVAEVRAIGDNTNCMALKGASPEHEGEERPDRWPLTPELEQLAERWHIAPERDLVKS
                     AA"
BASE COUNT          552 a         1413 c         1340 g          653 t
ORIGIN      
        1 gcggctgccg ttggcgcgtt aacgtacgcg ccgatgtcga acctcgccct ctggctgatc
       61 ctcgccggcg tgccgctcat ctttggcctg tgggcgcaga cgcgggtgaa gaaaaccttc
      121 aaccgctaca gcgacgtgcc gaccgcgaac ggcgtgaccg gtgcgcaggt gggcgaggcg
      181 gtggtgcgat cgtcgggtct ccctgacgtg tcggtccgac cggtgcaggg acagctcacc
      241 gaccactacg acccgcgcac acgcactctc aacctgtccg agggcgtgta ccagtcgcac
      301 acggtggcgg ccgccggcgt ggcggcgcac gaggcgggcc acgccatcca ggacgcacag
      361 cactaccgcc caatgcgtat acgccagacg ctcgtgccgg cggcgcagat cggccagtcg
      421 ctgtggttcc tgccggtgtt catcgggctg atcatcggcg ccaccggcct ggtcacgatc
      481 gggctgatcc tgttcgcggc cgtcgtgctg ttccagctgg tcacgctgcc cgtcgagttc
      541 gacgcctcca agcgggcgct cgtcgcgctc gagcatcagg cgatcctcgc gcctcaggag
      601 ctgaacggcg cacgcgaggt gctgcgcgcg gccgcgctca cctacgtggc cgggttcgtc
      661 gccgcgctcg ggcagctcct ctacttcttc atcgcctcac gccgctagcg agtcgagtac
      721 cgcggcgagc gcgcgcgggt cccgccgcga gcgccggcct tccgtgaggt gggccacata
      781 agcggagcga ccgccgggac gcatagcggg ggcgtcccgg ccgcgatgac tctcagtgac
      841 gcttagtgta cctaatcgtt tgctaggcta aagatcgtga gcacaactac cactagagcg
      901 cagaagcggg ccgcgatcgc ggccttcaac cagtcgttcg acgacttcgt cgacgcaatt
      961 cgccgcgcac gcgggcgcgc cgaacgcgac cgctcggagg gcctttcgct gtcacagttc
     1021 aagctgctgc agccgctgct cacgtttgag ccgcgccccg tcggcgagct cgccgccgcg
     1081 gcctgcgtct ccgcgccgag cgccacacgc atgatcgacg gcctagagcg cgatggactc
     1141 gtcacgcgcg agcactcgcc tgaggaccgc cgcgtggtgg cggtccggct caccgacgag
     1201 ggccgccagg ccgtcgaggc caagcgcgac tacgtggagg gcaagcgccg cgcgatctac
     1261 gagagcctca gcccgaacga gcgggagcag gcgcagcgcc tgctcacgca catcgctcag
     1321 gtgatcgatg agctctgacg ccggagtcgg gcgccagcac tacaacgtca cgctcggggt
     1381 cctcgcgatc tccgcactgg cgtacgcgct ctcgcagacc atggtggcgc cggcgctgcc
     1441 ggacatccag cacacgctgg ggacgtcgac caccacggtc acgtgggtgc tcaccgcgta
     1501 cctgctgagc gcgtcggtgg ctacgccgat catcggccgt ctcggcgaca tcttcggcaa
     1561 ggagcgcgtg ctcgtgctca cgctgctcgt cttcggcttc ggctcgctcg tggccgcgct
     1621 ctcacactcg atcggcatcc tcatcctcgg ccgcgcgatt cagggcaccg gcggcgcggt
     1681 gttcccgctc gccttcggga tcatccgcga cgagttcccg cccgggaagg tggcatcggg
     1741 catcggcctg atcagcgcca ccttcggcat cggcggcggc gccggcctcg tgatcagcgg
     1801 cctgctggtg gaccacctga actacgagtg gatcttctgg ctcggcctga tcttcgtcgc
     1861 gatcgcgatc gtggccacgc accggtacgt accggagtcg ccggtgaagg ccaaggcgaa
     1921 gatcgattgg ctcggcgccg cactcatgtc cgtcggcctc gtcgcgctgc tgatcggcgt
     1981 gagcgagggc aactcctggg gctggggctc tgaccgcgta ctcggcctgt tcgccgcatc
     2041 gatcgtgatc ctcgcgatct gggtctgggt cgagacgcgc gtggaggagc cgctcgtgga
     2101 catgcggatc atgcgcgagc gtgcggtgtt caccaccaac ctgacggcgc tgttcgtggg
     2161 cttcggcatg ttcggctcgt tcatcctcat ccctcagttc gtgcaggcgc cgctgtccac
     2221 cggctacggc ttcggcgcgt cggtcacgac ggccggcctg ttcctgctgc cctcggcgct
     2281 agtgatgctg ccggccggcc cgatcgccgg cacgctcggc gagcgctacg gctcgaaact
     2341 gccgctgctc gccgggagcg tgctggcggc actcgcctac ctcttcctcg cggtcgcgca
     2401 cgaccagcgc tgggagatct acgcggccac gacgctgctc ggcttcggca tcggcttcgc
     2461 cttcgccgcg atggcgaacc tcatcgtcga ggccgttccg caggaccaga ccggcgtcgc
     2521 gaccggtatg aacacgatcg cccggacgat cggcggcgcg ctcggcgcgc agctctcagc
     2581 gacgattctc gcggccgatc atctcgcgag cgggtaccca gccgagtcgg gctacaccgg
     2641 cgccttcgcg atgtcggccg tcggcgtggc tgccgccttt ttcgtcggtc tggcgatccc
     2701 ggcgcggcgc cacccagccg ccgcgctcgc tcacgaaggc gcgtagattc gccgcatggc
     2761 gaagctcccg gacgcgcgct ggtggtcgct ccggcgggca cagaaccgca agccggccac
     2821 ctacacctgc ccattctgcg gcggctacct cgcgtcgatg agcgagcaca tgctgatcgc
     2881 gccggagggc gactcctcgc ggcggcggca cgcgcacatg gcgtgtgtct tggcggcgcg
     2941 gaaatcgggg cgtttgccta cgcgcgacga gtggttgcgc tccgagcgcg gcgagcggcc
     3001 cagcttcgtt gcccggttgc tcgggcgttt acagcggcag taaggggacc accctctgcg
     3061 ctctggtcga tctgtgaacc tttgacccgg ctatagccgg gcaaagcgtt cacacatcgc
     3121 tatgccgcgc tcttcacaag gtcgcgctcg ggcgcgatgt gccagcgctc cgccaactgc
     3181 tcgagctcgg gcgtgagcgg ccagcggtcg ggacgctcct cgccctcgtg ttcaggcgag
     3241 gctcccttca gtgccatgca gttcgtgttg tcgccgatgg ctcggacctc ggcgacctcg
     3301 tccggcgaga gaacggcctc cgcgggcagc gacgccagct ccgcgcgctt ctcctcgatc
     3361 ggccgcgcgc cctcgcccgc ctcctggatc agcgtggggg ccaccacgcg taccggcggc
     3421 tgcgagaggt tccactcaca ggcaagctgc atcggcgtca ggccgtggcg ctccgcgatc
     3481 gggcgcatgc gctcgagccg ctctcgcccc gcctcgaccc agccctccgg ccggaacttt
     3541 cggtggtcga actgcgcgaa ctggtggccg tcgcgcacgt cgtcccagaa cagcccgccg
     3601 tagtcgacca cgcgcgtgat cagctcgacg ccgtgctcgc ttgctgcagg gagcgccagc
     3661 tcgccaggcc acggctccat cgggttgagg atgatcatcg cccagtcgat cgaggagccg
     3721 aagcgctcga aacagccgat gaggtcgagc gtgaagccat tggccgggcc gggcgcgacg
     3781 ccgagttgcc gcgcgagccc cacgccgcgc accgactcga gcgccttcca caccgcctcg
     3841 ctcgtgtagc cgatgcggtc ggggttgtga aggagcagca ggtcgaagga gtcgacgccg
     3901 cagcgctcga ggctgcgctc agtggccatg cgaacgtagt ccgcgtagcc gtcaggcc
//
LOCUS       DASVXS010000028        20181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_927723, whole genome shotgun sequence.
ACCESSION   DASVXS010000028 DASVXS010000000
VERSION     DASVXS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927723"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2608
                     /gene="gyrA"
                     /locus_tag="VF752_01410"
     CDS             <1..2608
                     /gene="gyrA"
                     /locus_tag="VF752_01410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8854228.1"
                     /translation="LTEVTTPPPGDRVEPVGLEVEMQRSFLDYAMSVIVSRALPDVRD
                     GLKPVHRRVLYGMNEAGMQPNRPYKKSARIVGDVMGSFHPHGDQAIYDTLVRLAQDFA
                     QRYPLVDGQGNFGSIDNDPPAAMRYTESRLARLALEMLRDIDADTVDFGPNYDESRLE
                     PLILPSRFPNLLVNGAQGIAVGMATNIPPHNLRETVDAVVAYIDDPQIGIAGLMKHIK
                     GPDFPTGGIIIGHGGIKDAYETGRGRVVVRARAHIEPLRQGKEAIIVTEMPYQVSKGD
                     GRNDGSGLIRKIAEVVNDKKVPEVSDLRDESDKSGTRIVIELKRDAIPKVVLNKLYKH
                     TSMQTTFGVNMVALVDGVPRTLGLEQLIKNYVDHQREVIVRRTKHELREREARLHILE
                     GLLVALTDLDAVIELIRASRDPEAARNGLMERFKLSQIQAQAILDMRLARLTALEADK
                     VRQEHADTLERIKELREILGDEERVMTLIKEELLEIKDRFGDERRTEITLSEDEIDIE
                     DLIADQQMVISITKSGYIKSLPLSTYRQQKRGGVGVIGMDMKDEDYIEHLFVTSSHDY
                     LLFFTNRGKVYRQKVYELPEAARTAKGRALVNLLPLREGERVHAVLSTRDFNEGKYLV
                     FATRKGQIKKTEFQAYNTPIKADGIIAINIRDDDELVAVRRTSGDDDIIMVSRSGQAA
                     RFNESEARPMGRDTGGVRGMNVSRGENAVIAMDIARDDTELLVVTENGYGKRTPISDY
                     PRKGRGTMGVKTISLTEKKGALAGALIVREHDGLVFISQNGMVQRTGVKGISRYGRAS
                     QGVRVMNMREDDVVSAVALVMEDSTPGAAVQEGIEAADTDGDVVAIDAVENAEAVEDS
                     SDGASPGADDQE"
     gene            complement(2934..3290)
                     /locus_tag="VF752_01415"
     CDS             complement(2934..3290)
                     /locus_tag="VF752_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854229.1"
                     /translation="MIEVERIDFISVAVRDRVRARRFYGDVLGLPENERTPDEFEAGN
                     VTLGLWEPEADGVPFAPNGAGLALRVPDVDEARSRLEAAGVEFIGETVDTGVCHMGFF
                     KDPDGNVLILHRRYAD"
     gene            complement(3303..3920)
                     /locus_tag="VF752_01420"
     CDS             complement(3303..3920)
                     /locus_tag="VF752_01420"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8854230.1"
                     /translation="MAKTSTGSAALVVEETSEQPGPVAVLPAERPGQHGSSTRSRVLV
                     LNASYEPINVCTVRRATVLVLKRRAEVVEESDWALHAESFTMQRPLVIRLTAYVKIPR
                     DAHSRKITRRAVFARDRWTCQYCGSERGSLTVDHVVPRSKGGGSGWDNIVACCAPCNR
                     RKGDRLPKDANMHPRRRPKAPHSTIFVYVATPSIPDAWHPYLIAA"
     gene            complement(3975..4811)
                     /gene="pheA"
                     /locus_tag="VF752_01425"
     CDS             complement(3975..4811)
                     /gene="pheA"
                     /locus_tag="VF752_01425"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX8854231.1"
                     /translation="MRVAFLGPAGTYAEEALRASTPEDVEEIPHPTVYEAVMAVQDGD
                     ADRAVVPIENSLEGSVSATLDVLAAEATGVRIAAEVDLPIHHCLIARDSTELGAIERV
                     LSHPQATAQCARFLRERLPDAERTSTPSTAEAVRMVAAEDRPWAALGSRFAAELYGCQ
                     VLDEQVADSEDNVTRFVWLAPSAEPADPDATKTSIVFWGFNDQSPGALVDVLRELADR
                     NINLTKIESRPRRVQLGHYMFFADLDGAEHEPVIREALEGLRTRVETLRVLGSYSAIA
                     AR"
     gene            complement(4813..5319)
                     /locus_tag="VF752_01430"
     CDS             complement(4813..5319)
                     /locus_tag="VF752_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX8854232.1"
                     /translation="MDDLTSTAGIVALAAAGVAVVAFLFAIVLAVKLRRMRTAQQAVL
                     GDGQRDLVDHATRLERGFVDLREWVEDAMHRLDERLGTAEERIDGCIAYSALIRYDAY
                     GEMSGRQSSSIALLDSRRSGVVLSSILHREQARLYAKPIRNGESEFDLSPEEVEAIEA
                     ALNAGARA"
     gene            complement(5351..6532)
                     /locus_tag="VF752_01435"
     CDS             complement(5351..6532)
                     /locus_tag="VF752_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8854233.1"
                     /translation="MPPGRASYWRNPGQIACLGSKPPEEQLLNFLEEREERSSRSTSG
                     RRPPRRSSGDAQTVRTRRLIALGVGILVLFLIGLGIKGCLDARKKQAFKDYGRDVAAL
                     LQESDQQSKDLFDLLRGTQQTSTPVDVQSAVNGARVQAEQLVERAKGISHPGELSTAQ
                     RYLEETLSFRRDGLTGIARLLPTALGDQGRAEAANQIAGQMRAFDASDVIYSQRFTPE
                     FQQQIKNESLTGEVTVPKSQFLPDIEWLRPNVVSDRVARIRGGSSASQPATPGLHGTG
                     LGSVVVKPGGQTLASAQVADIPVSPNISFDVQVVNQGENDEQNITVTVKLSGAGKPIT
                     VTEKLPQIARGETKTVNLPLAATPTTGQPVTINVSVSPVPGEKKTDNNKATYRSVFTS
                     G"
     gene            6571..8391
                     /locus_tag="VF752_01440"
     CDS             6571..8391
                     /locus_tag="VF752_01440"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8854234.1"
                     /translation="MERPPPTDELEPTTAAMPTAPTRRFAREPVPDGFGGELLLERYR
                     LERRLGAGGFGTVWLARDERLERDVAVKVIPRGDSDEGSPRAQREATAAARLNHPGIV
                     ALYELASDDDHVYLVSELVHGSTFAELLREGALSDRDVANIGVALCEALAHAHSRGVV
                     HRDVKPQNVMVVAEPAAGAGFAKLADFGVAHLADVEPLTHTGDVVGTLAYMAPEQAEG
                     ERVSEAADVYSLALTLYEGWAGRNPVRAASPAATARRLGRRLPSLASRRRDLPLELCA
                     AIDTALDPDPDRRLPLDLLREELERTERELSDEGGLVEPENIERFGLRRADRPLRLAW
                     PARLTAGVAAGALVAAAIALLGPPAPASPAAIAAGCALLVAVLPRAGWLAAAAALLGW
                     LIGEGEPGTALVIAPALAAVPLLLPRSPLVWSAPVLAPLLGLIGLAPAFAALAGFGGR
                     LRRRAALAVAGVIWLVAAEALTGRSLLYGPADGTQPRSDWAGSLTKSAEHAVWPAISS
                     PALLSALAFGLLAALVPLAVRARNLTADIVLGLLWSAGLVAALRALDDVMDPHMRLGS
                     ARGAVAGALTGAVAAIALSRVRRDRSGPDSHESSLGWPSE"
     gene            8388..9134
                     /locus_tag="VF752_01445"
     CDS             8388..9134
                     /locus_tag="VF752_01445"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX8854235.1"
                     /translation="MSVLRNLEQKIEAMVEGAFSRAFKSRVQPVELARKLAKEMEENQ
                     TVSISRVYVPNEYCVFLSPDDREQFSGYEDALRKELSDYLLEHARNENFALVTRPTVD
                     FRTDERLSLGEFGIQAQLVRAPEDEAGEPEAGDFGHTMVYSPDREARKLVPPGGTGRA
                     MLVGAGRRTVLSGERVVMGRSRDSDIVLDDPNVSRQHAELRNRGEGWVVRDLGSTNGI
                     KVNGRRVEQASLQPGDEITLGLSRLTFEQE"
     gene            9138..9632
                     /locus_tag="VF752_01450"
     CDS             9138..9632
                     /locus_tag="VF752_01450"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8854236.1"
                     /translation="MTDPVAVALKFGFLIVLYLFLLWVARSSMRDLSRHEDASMVMEG
                     APRNAAARSAERQRPAGPDLRAGVRPRLEVVAAMGHDPGARFDISQGATLGRSNASDI
                     EVNDPFASSAHARIFPRGQFMYIEDMGSTNGTYLNGRQLRRPEQLKVADTVRIGDTEY
                     RYQE"
     gene            9637..10869
                     /locus_tag="VF752_01455"
     CDS             9637..10869
                     /locus_tag="VF752_01455"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX8854237.1"
                     /translation="MGLRIVEEAALTDVGRQRNANEDAYYESSPVFAVADGMGGARAG
                     EVAARIAVEEFDQVADETASPEELLAGIARSANKRIYELAQRDETHAGMGTTFIAAMV
                     SGNEVAVGHVGDSRAYRFRDEELERLTQDHSLVEELRRQGKLTEAQAEAHPQRSIITR
                     ALGPEPDVDVDTFSFPARDGDVYMLCSDGLTGMVSEERIAQILRVRSSLRQAAEELVS
                     EANARGGRDNITVVLFKLGGDEERDQDSDTLSGQETSVALGDGAPAADPAHETMAMPA
                     AEANAARAAVAEREAPPPEAPAAEPPPAQPARRGKRWLKAAVGVLVVAAVLAGLWIGS
                     RQFWFVGTDNQGAITLFRGLPYELPLGVKLYTEEYQSSVPALSLPKLQRQRILDHQLR
                     GRSDAVDLVRSLEQTHAS"
     gene            10869..12170
                     /locus_tag="VF752_01460"
     CDS             10869..12170
                     /locus_tag="VF752_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX8854238.1"
                     /translation="MSARTREFFGLIPVALMVTAGFTAVLITRSSQISTATLTYGAVF
                     LGLCVFAHFFVRARLPDADPYMFPLAALLAGFGLVMIYRINPDLARKQAQWFVFGLIV
                     FCATIVFLRDHRTLERYRYTIAAVSIALLLAPRLPGIGGATNGAYLSVSFGAFQFQPA
                     EFAKIGVIIFLASYLRDTRDVLVRGRFPHLSLKHFGPLLIIWGAAMLMLIFIRDLGSS
                     LMFFGAFLALLYVATSRLSFVAGGLGLFALGAWFLGSHIQHVQDRVTVWKDPFKPGLV
                     EHEGYQIAQGLFAQADGGLFGKGFGQALLQSPYGGTLLPAPHTDLIYAVITDELGLFG
                     AVGLLVVYLLFIDRGFKTALVAGDAFSKLLATGLTAVVAIQVFVIVGGVTKVIPLTGV
                     TLPLVSYGGSSIVANLLLLALLLIVSDHARREPHAAGGVVR"
     gene            12167..13612
                     /locus_tag="VF752_01465"
     CDS             12167..13612
                     /locus_tag="VF752_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8854239.1"
                     /translation="MNRQITQVFGLMLILFAVLVGASSWWTVFGADGLKDNSANRRDL
                     LQEQRIPRGLILTDNGDTAARSTRLGSGNSLRYVRTYPTGNLFSHVFGYSFIQLGRAG
                     LERSENDALAGNQNEFSSIVDELLNRPQEGFDLHTTLDAGAQRLARQLLAGKGRGSVV
                     AIEPKTGRVRVMLSLPDYDPNNIPQDFKQLNRDPSSPLFNRATQSGYPPGSTMKVVTA
                     SAALDSGRYTPNSVVSGKSPKIVGGVPLSNCCTEGSGNFGALTLTEALAKSVNTVWGE
                     VGEKLGKNTMFEYMRRFGFDSKPPIDLPRDELRSSGVYGRGGKLLDEHDAIDIGRVAI
                     GQERLNVTPLQMAEVAATIANGGVRMRPQLVDRVVARDGRVKERRKPEEVQRVIKEST
                     AREVTQMMQGVVANGTATAAQIPGVQVAGKTGTAEVDNGAANQAWFIAFAPANDPKVA
                     IAVTVERTQGEGGTVAAPIAKQIMQYLLGKG"
     gene            13612..15459
                     /locus_tag="VF752_01470"
     CDS             13612..15459
                     /locus_tag="VF752_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX8854240.1"
                     /translation="MAEVAENTVVDNRYRILNRIGSGGMADVYCAEDTHLGRQVALKV
                     LHRRFAQDDEFVERFRREASAAAALQHPNVVNVYDRGKHDGTYYIAMEFLQGRTLKEI
                     IQAEAPLSQERAIELAIPILRAAGFAHKRGVIHRDFKPHNVMVSPSGAVKVTDFGIAR
                     AGASEMTETGSIMGTAHYLSPEQAQGHGVTAASDLYSIGVMLYEMLAGQVPFQGDSAV
                     SIALKHLSEPPPRLAQFARVNPRLEAVLNRALAKQPEQRFQSADDFIAALEEARAAIA
                     AGDNGDAQDTATWTAVPPPPIAPPPAERGRRRWPWVVLAVLLAAGIAAGVVALVLGGN
                     RVTVPKVVGETIEQASPQLTQKGFHVRTTRRTSEQSAGLVLAQDPAGGDKADKGSTVN
                     LTVSTGPGKKTVPDVRGMTGQEALTALSKRGFLVEEHQRHSTSVKDGFAIGTDPANGT
                     SQEVGSRVQLFISSGPRQVAVPDVVGLDVDSAKSTLSDQGFTYTTTQQASDQPEGRVL
                     SQTPSSGTKVDEGSRVSLVVSKGPDTADIPAVVGLTEQQATDQIRAAGFEVQVKDKSV
                     NDPTQDGQVVQQRPKSGKLKKGRTVVIYVGRAAQQPGGTGTPPMGGGTG"
     gene            15459..16427
                     /locus_tag="VF752_01475"
     CDS             15459..16427
                     /locus_tag="VF752_01475"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX8854241.1"
                     /translation="MRVAVLAGGRSSEHDVSLASATSVAEGLAQAGHEPLLVEISREG
                     VWSLDGEPLALTPGKQVAGADIVFPVLHGPYGEDGTVQGLLETIGTPYVGAGVLSSAL
                     CMDKVVFKELMSQAGIPQVDYVALREGDDSRLASRLGMPLFVKPARLGSSVGISKVSS
                     GEAELEQALSLAWEHDPVALVEAFSGGMEVECSVIGNDAPETSQPGEVVPHADWYDFE
                     AKYSPGGMELVVPPRLDEHAIERVRALAVEVYRLVGCSGFARVDFFVENGHNVLVNEL
                     NTIPGFTPTSGFPKMFEASGVPYPELLDRLLGLGMERFERERRYGF"
     gene            complement(16415..17911)
                     /locus_tag="VF752_01480"
     CDS             complement(16415..17911)
                     /locus_tag="VF752_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8854242.1"
                     /translation="MIGTLLSGRFRLEEKIGSGGMSTVYRAFDETLERWVAIKLLHSE
                     ISSDSDQIERFRREARAVARFSHPNVVTVIDAGEDDGQPYIVFEYVAGETLKERIRRL
                     ARLPVPEAIAYAIEIGRALEAAHAERLVHRDVKPQNVLIDADGRAKVTDFGIARSLEA
                     DGLTAAGRVLGTTDYVSPEQALGEPVTPQSDIYSLGVVLYEMLTGDVPYRADAPVAVA
                     MKHVREPMPDVQRMRPEVSAATAAIVDTATAKETRNRYRSVADMLTDLEQALAIEAAR
                     AGQTSGEATTVLRALPDKTTQRFLPWRLRSPRRALVITALLAGGIALAAILLLTQTKK
                     GSPGSAPPSKASATLRTVSFSPTGVHDYDPLGDRREHPEAVRNVIDDNPSTTWTTEQY
                     TGGNLGKPGVGLYLDAGHGVLARWLDLTTGPGSWTMSVYGTSADPVPQTIAGWTKLSG
                     DQTVNGPSHVKLNTGGKQYRNYLIWITKLPPSGRAEIREVTLRVTQNP"
     gene            17933..18814
                     /locus_tag="VF752_01485"
     CDS             17933..18814
                     /locus_tag="VF752_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8854243.1"
                     /translation="MPVRVRYFTDPACSWSWNNEPVVRKLMVEFGTELSWSWVMGGLA
                     RDYTQGLSGGDAGASGDDAVYAFLTRRWLQAAEWGRMPLDPLIWHEGPLSSSYPACMA
                     VKAAAEQSPDDDGYRYLRALREGILCFRRKLDTTEALVEEARRTGLDVQRFRIDLGSH
                     AIVEAFGADLELTRNPPETARREGKVEQRHGGERLPFPSAVFIGEDGSEHGVFGRAPY
                     ETWAAAAEAAGARRTREEQLSPLAAVHRFGRIATAEVEAVCDLPTPRAAAKLWELASD
                     WKLKPVRVLTGWLWEPA"
     gene            complement(18811..19296)
                     /locus_tag="VF752_01490"
     CDS             complement(18811..19296)
                     /locus_tag="VF752_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8854244.1"
                     /translation="MASGPIVIGFDGSEAAERAVREAGSVLTGKPALVVAVWEPGTAL
                     EYTTSSLGVDAALPPIDVRASLEVDQALAEASQRTAQRGAEIARQAGFEAEALAVADE
                     VSVAQTLVRLARERDSAAIVVGSRGQGGLKSLVLGSTSHGVLQHADRPVLVVRAAADE
                     D"
     gene            complement(19306..>20181)
                     /gene="purM"
                     /locus_tag="VF752_01495"
     CDS             complement(19306..>20181)
                     /gene="purM"
                     /locus_tag="VF752_01495"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX8854245.1"
                     /translation="SKVILAEQLGRFDTVGIDCIAMNVNDLVCVGAEPIALVDYIAVE
                     AANPEMLRQIGEGLKAGAEQAGIEIPGGELAQLPELIRGHPSPFGFDLAASAFGTVQL
                     DRVVTGDHIEPGDALIGVPSSGLHSNGYTLARSVLSELDETPRELGGASVGEVLLEPT
                     VIYVRAVLELLRSETAVRGLAHITGDGMLNLLRLNDDVGFRVDEPLPAPPVFGLIAER
                     GGIAPADLWETFNMGVGMVAVVPEADAERATQLLAKHHPGAARIGSVTSEAGLVEIPS
                     AGITGRRERGFAPNP"
BASE COUNT         3068 a         6968 c         7075 g         3070 t
ORIGIN      
        1 gttgactgag gtcactactc cgccgcccgg agatcgcgtc gagccggtcg gtctcgaagt
       61 cgagatgcag cgcagcttcc tcgactatgc gatgtccgtc atcgtctcgc gggcgctgcc
      121 cgacgtgcgc gacggcctga agcccgtgca ccggcgcgtg ctgtacggca tgaacgaggc
      181 cggcatgcag ccgaaccggc cgtacaagaa gtcggcgcgc atcgtcggcg acgtgatggg
      241 ttcgttccac ccgcacggcg accaggcgat ctacgacacg ctcgtccgcc tcgctcagga
      301 cttcgcgcag cgctatccgc tcgtcgacgg ccagggcaac ttcggctcga tcgacaacga
      361 cccgccggcg gccatgcgct acaccgagtc gcgcctcgcg cgcctcgcgc tggagatgct
      421 gcgcgacatc gacgccgaca ccgtcgactt cgggccgaac tacgacgagt cgcggctcga
      481 gccgctgatc ctgccttcgc gcttcccgaa cctgctggtc aacggcgcgc agggaatcgc
      541 ggtgggcatg gccacgaaca tcccgccgca caacctgcgc gagacggtcg acgcggttgt
      601 cgcctacatc gacgacccgc agatcggcat cgccggcctg atgaagcaca tcaagggccc
      661 ggacttcccg accggcggca tcatcatcgg ccacggcggc atcaaggacg cctacgagac
      721 cggccgcggc cgcgtggtcg tgcgcgcacg cgctcacatc gagccgctgc gccagggcaa
      781 ggaggcgatc atcgtcaccg agatgcccta ccaggtgtcg aagggtgacg gccgcaacga
      841 cggaagcggg ctgatccgga agatcgccga ggtcgtgaac gacaagaagg tgcccgaggt
      901 ctcggacctt cgcgacgagt ccgacaagag cggcacgcgc atcgtcatcg agctgaagcg
      961 cgacgcgatc ccgaaggtcg ttctcaacaa gctctacaag cacacgtcga tgcagacgac
     1021 cttcggcgtg aacatggtcg ccctggtcga cggcgttccg cgcacgctcg ggctcgagca
     1081 gctgatcaag aactacgtcg accaccagcg cgaagtcatc gtccgccgca cgaagcacga
     1141 gcttcgcgag cgcgaggcgc ggctgcacat cctcgaggga ctgctcgtcg cgctcaccga
     1201 tctcgacgcc gtgatcgagt tgatccgcgc ctcgcgcgat ccggaggcgg cgcgcaacgg
     1261 cctgatggag cggttcaagc tctcgcagat ccaggcgcag gcgatcctcg acatgcgcct
     1321 cgcacgcctc accgcgctcg aggccgacaa ggtcaggcag gagcacgccg acacgctcga
     1381 gcgcatcaag gagctgcgcg agatcctcgg cgacgaggag cgcgtcatga cgctcatcaa
     1441 ggaggagctg ctcgagatca aggatcgctt tggcgacgag cgccggacgg agatcacgct
     1501 ctcggaggac gagatcgaca tcgaggacct gatcgccgac cagcagatgg tcatctcgat
     1561 cacgaagtcc ggctacatca agtcgctgcc gctctccact taccgccagc agaagcgggg
     1621 aggcgtcggc gtaatcggga tggacatgaa ggacgaggac tacatcgagc acctcttcgt
     1681 cacgtcaagc cacgactacc tgctgttctt tacgaaccgg ggcaaggtct accgccagaa
     1741 ggtctacgaa ctgccggaag ccgcgcgcac cgccaagggc cgcgcgctcg tgaacctgct
     1801 gccgctacgg gagggcgaac gggtgcacgc cgtgctctcc acgcgcgact tcaacgaggg
     1861 caagtacctc gtcttcgcga cgcgcaaggg ccagatcaag aagaccgagt tccaggccta
     1921 caacacgccg atcaaggccg acggcatcat cgcgatcaac atccgcgacg acgacgagct
     1981 cgtggccgtg cgccgcacga gcggcgatga cgacatcatc atggtgtcgc gctccggaca
     2041 ggcggcgcgc ttcaacgagt cggaggcgcg gccgatgggc cgcgacacgg gcggtgttcg
     2101 tggcatgaac gtctcgcgcg gcgagaacgc cgtgatcgcg atggacatcg cgcgcgacga
     2161 caccgagctg ctcgtggtga cggagaacgg ctacggcaag cgcacgccga tctcggacta
     2221 tccgcgcaag ggtcgcggca ccatgggcgt gaagacgatc tcgctcaccg agaagaaggg
     2281 cgccctcgct ggtgcgctca tcgtgcgcga gcacgacggc ctcgtcttca tctcgcagaa
     2341 cggcatggtt cagcgcacag gcgtgaaggg catttcgcgc tacggccggg cgtcacaggg
     2401 cgtgagggtc atgaacatgc gggaggacga cgtcgtgagc gcggtcgcgc tcgtgatgga
     2461 ggactcgact ccgggtgccg cggtgcagga gggcatcgag gctgccgaca ccgacggcga
     2521 cgtcgtcgcg atcgacgcgg tggagaacgc ggaggccgtc gaggactcga gcgacggcgc
     2581 gtcgcctggc gccgacgacc aggaatagcc ggccgccgaa acgtagagag ggcgcccccg
     2641 aaggagcgcc ctctcgagca tgctgaacct gatcggcgac gtttttcgcg gcgaggcggc
     2701 ctggtgagcc gaacgccggg cgagcgggac ctacctctcg cctatgcctg ccgacggtgc
     2761 acgggttgga ctccgcaaga cgggtccagc tgcgtgctag cggccctgca cctccagggt
     2821 cccaaaacta ccgtctgaca agttcggacc acctcctttc cctggtagcc cttatgtaag
     2881 cagacgggca ggcggagtcc cagatgacgc gcgcgccatt tttctcgtac cggctagtcg
     2941 gcgtagcgtc ggtggaggat caggacgttg ccgtccgggt ccttgaagaa gcccatatga
     3001 cacacgccgg tgtcgaccgt ctcgccgatg aattccacgc ccgccgcctc gagccgcgag
     3061 cgagcctcgt cgacgtccgg tacccgcagt gcgaggccag cgccgttcgg agcgaacggc
     3121 acgccgtcgg cctcaggctc ccacaggccg agcgtcacgt tgcccgcctc gaactcatcg
     3181 ggcgtgcgct cgttctccgg caggccgagc acatcgccgt agaagcgccg cgcgcggaca
     3241 cggtcgcgaa ccgcgaccga gatgaagtcg attcgctcga cctcgatcat cggtttgccg
     3301 cgtcaggcgg cgatgaggta cgggtgccac gcgtccggga tgctcggcgt ggcgacatac
     3361 acgaagatcg tggagtgcgg cgccttcggc cgccggcgcg ggtgcatgtt cgcgtccttg
     3421 ggcagccggt cgcccttgcg gcggttgcag ggcgcgcagc aggcgacgat gttgtcccag
     3481 cccgacccgc cgcccttgga ccggggcacg acgtggtcga ccgtcagtga gccgcgctcg
     3541 gagccgcagt actggcaggt ccagcggtcg cgcgcgaaca cggcgcggcg ggtgatcttg
     3601 cggctgtggg cgtcccgcgg gatcttcacg taggcggtga gccggatcac gagcgggcgc
     3661 tgcatcgtga agctctcggc gtggagggcc cagtcgctct cctcgaccac ctcggcacgg
     3721 cgcttcagca cgagcacggt cgcccgccgc acggtgcaga cgttgatcgg ctcgtacgag
     3781 gcgttcagga cgaggacgcg gctcctggtg ctgcttccgt gctggcccgg ccgctcagcg
     3841 ggcagcacgg ccactggccc aggttgttcg ctcgtctcct ctacgacgag cgccgcgctg
     3901 ccggtgcttg tcttcgccac ccttggctga gtgtactcgg cgaccccggc gtttccgcgc
     3961 cggttcacga gtccctaacg tgccgcgatt gcgctgtatg agccgagcac ccggagcgtc
     4021 tccacgcgag tgcggagacc ctcgagcgcc tcgcggatca cgggctcgtg ctcggcaccg
     4081 tcgaggtcgg cgaagaacat gtagtgcccc agctgcaccc tgcgcggccg ggactcgatc
     4141 ttcgtgaggt tgatgttccg gtcggcgagc tcgcggagca catccacgag cgcgcccggc
     4201 gactggtcgt tgaatcccca gaacacgatt gacgtcttgg tcgcgtcggg gtcggcaggc
     4261 tcggcgctcg gcgccagcca gacgaagcgc gtcacgttgt cctcgctgtc ggccacctgc
     4321 tcgtcgagca cctggcagcc gtagagctca gccgcgaagc gcgagccgag cgccgcccac
     4381 ggacggtctt cggcggcgac cattcgcacg gcctcggccg tcgacggcgt gctggttcgc
     4441 tccgcgtccg gcagccgctc gcgcaggaag cgcgcgcact gcgcggtcgc ctgggggtga
     4501 gagagcacgc gctcgatcgc gccgagctcc gtgctgtccc gcgcgatcag gcagtggtgg
     4561 atcggcaggt ccacctcggc ggcgatgcgg acgccggtcg cttcagcggc caacacgtcg
     4621 agcgtcgcgc tcaccgagcc ctcgagcgag ttctcgatcg gcaccacggc acggtcggcg
     4681 tcgccgtcct gcaccgccat cacggcctcg tacacggtgg ggtgcgggat ctcctcgacg
     4741 tcctcggggg tggacgcccg cagcgcctcc tccgcgtagg tgcccgccgg ccctaggaac
     4801 gcgacgcgca cttcaggcgc gagcgccggc gttcagtgcg gcctcgatcg cttcgacctc
     4861 ctcgggggag aggtcgaact ccgactcgcc gttgcggatc ggcttggcgt agaggcgcgc
     4921 ctgttcgcgg tggaggatcg acgacagcac gacgcccgag cgccgcgagt cgagcagcgc
     4981 gatcgagctc gactgccggc ccgacatctc gccgtacgcg tcgtaccgaa tcagcgcgct
     5041 gtacgcgatg cagccgtcga tccgctcctc agccgtgccg agccgctcgt cgaggcggtg
     5101 catggcgtcc tccacccact cgcgcagatc cacgaaaccg cgctcgagcc gggtcgcatg
     5161 gtccacaagg tcgcgctggc cgtcgccgag cacggcctgc tgggccgtgc gcatgcgccg
     5221 cagcttcacc gccaggacga tcgcaaacaa gaacgctacg accgcgacgc ccgccgcggc
     5281 gagcgccacg atgccggcag ttgacgtcag gtcatccacg acgcctcaat gtacaaggcc
     5341 tgcgcgcgac tcagcccgac gtgaagacgg acctataggt cgccttgttg ttgtccgtct
     5401 tcttctcacc cggcaccggc gacacggaca cgttgatcgt caccggctgg cccgtcgtgg
     5461 gcgtcgccgc cagcggcagg tttaccgtct tggtctcacc gcgggcgatc tgcgggagct
     5521 tctcggtgac ggtgatcggc ttgcccgcgc cgctcagctt cacggtcacc gtgatgttct
     5581 gctcgtcgtt ctcgccctgg ttcacgacct gcacgtcgaa cgagatgttc gggctgacgg
     5641 ggatatcggc gacctgggca ctggcgagcg tctgtccgcc cggcttcacc accacgctgc
     5701 cgaggccggt gccgtggagg cccggcgtgg ccggctggct agccgacgac ccgccccgga
     5761 tgcgcgccac gcggtcggac accacgttcg gccgcagcca ctcgatgtcc ggcaggaact
     5821 ggctcttcgg aaccgtgacc tcgccggtga gactctcgtt cttgatctgc tgctggaact
     5881 cgggcgtgaa gcgctgcgag tagatcacgt cgctcgcgtc gaacgcgcgc atctggccgg
     5941 cgatctggtt ggcggcctcg gcacggccct ggtcgccgag cgcggtgggc agaaggcgcg
     6001 cgatgccggt cagcccgtcg cgccgaaacg agagcgtctc ctcgagatag cgctgtgccg
     6061 tcgacagctc accgggatgg ctgatgccct tcgcccgctc cacgagttgc tcggcctgca
     6121 cccgcgcgcc gttcacggcg ctctggacgt cgaccggcgt ggatgtctgc tgcgtgccgc
     6181 gcaggagatc gaagaggtcc ttgctctgct gatccgactc ctggagcaga gcggccacgt
     6241 cgcggccgta gtccttgaac gcttgcttct tgcgcgcgtc gaggcagccc tttatcccga
     6301 ggccgatcag gaacaacacg aggatcccga cgccgagcgc gatcaggcgg cgagtgcgaa
     6361 cggtctgcgc atcgcccgag ctgcgccggg gcggacggcg cccggatgtg ctccggctag
     6421 atcgttcttc gcgttcttcc aggaagttca ggagctgctc ctcaggcggc ttggagccga
     6481 ggcaggctat ctgacctggg ttacgccagt atgaagcccg ccccggcggc atggagggag
     6541 cgccgtcatc ggccagaatc acaagccgag atggaacgcc ctccgccgac cgacgagctg
     6601 gagccgacca cggctgccat gccgaccgcg ccgacgcgac gcttcgcgcg cgagcccgtg
     6661 cccgacggtt tcggcggcga gcttctgctc gagcgctacc gcctcgagcg ccgcctcggc
     6721 gccggcggct tcgggaccgt ctggcttgcg cgcgacgagc gcctcgaacg cgacgtggcg
     6781 gtgaaggtga tcccgcgtgg agattcggac gagggatcgc cgcgggccca gcgcgaggcg
     6841 accgccgccg cccggctgaa ccacccggga atcgtcgccc tctacgagct cgcgtcggac
     6901 gacgaccacg tctacctcgt ctcggagctc gttcacggca gcaccttcgc tgagctcttg
     6961 cgcgaaggcg cgctgtcgga ccgcgacgtg gcgaacatcg gcgtggcgct gtgcgaggcg
     7021 ctcgcgcacg cacactcgcg cggagtcgtt caccgcgacg tgaagccgca gaacgtgatg
     7081 gtggtcgctg agcccgcggc gggcgcgggc ttcgcgaagc tcgccgactt cggtgtggcg
     7141 cacctcgccg acgtcgagcc gctcacgcac accggggacg tggtgggcac gctcgcgtac
     7201 atggcgcccg agcaggcgga gggcgagcgc gtttccgagg cggcggacgt ctattcgctc
     7261 gcgctgacgc tctacgaggg ctgggcggga cgcaatcccg tacgcgcggc gagccccgcg
     7321 gccaccgcgc gccggctcgg ccgccgcctt ccgtcgctcg catcgcggcg gcgcgatctg
     7381 ccactcgagc tgtgcgcggc catcgatacc gctctcgatc ccgaccccga ccgccgcctg
     7441 ccgctcgacc tgctgcgcga ggagctggag cgaacggagc gcgagctgtc cgacgagggc
     7501 ggtctcgtcg aacccgagaa catcgagcgc ttcggactgc gccgggcgga ccgtccgctg
     7561 cggctcgcct ggccggcgcg cctcaccgca ggcgtcgcgg cgggagcgct cgtggccgcc
     7621 gcaatcgcgc tgctcggccc gcccgcgccc gcctcgcccg ccgcgatcgc ggctgggtgc
     7681 gcgctactcg tggcggtcct cccgcgagcg ggctggcttg cggcggcggc cgcgttgctg
     7741 ggctggctga tcggggaggg cgagccgggc acagcgctcg tgatcgctcc cgcgctcgct
     7801 gccgtgccgc tcctgctccc gcgctcgcca ttggtctggt cggcgccggt gctggcgccg
     7861 ctcctcggct tgatcgggct ggcgcccgcc ttcgcagcgc tcgcgggatt cggcggacgc
     7921 ctccggcgtc gcgcggcgct cgctgtcgcc ggtgtgatct ggcttgtcgc cgcggaggcg
     7981 ctcaccggaa ggtcgctcct ctacgggccc gccgacggga cgcagccgcg ctcggactgg
     8041 gcggggtcgc tcacgaagtc ggccgagcac gcggtctggc cggcgatcag ctcgcccgcc
     8101 ctgctgtccg cgctcgcctt cggtctcctc gcggcgctcg tgccgctcgc ggtgcgcgcg
     8161 cggaacctca cggccgacat cgtgctgggc ctgctgtggt ccgccgggct ggtcgcagca
     8221 ctgcgcgcgc tcgacgacgt gatggacccg cacatgcgcc tcggatcggc gcgtggcgcc
     8281 gtggctggcg cgctcacggg ggcggtcgcg gcaatcgctc tgagccgggt tcggagggat
     8341 cgcagcgggc ctgactcaca cgagtcttcc ctaggatggc cttcggaatg agcgtgctcc
     8401 gcaacctcga gcagaagatc gaggcgatgg tcgagggcgc cttcagccgc gcgttcaagt
     8461 cgcgcgtgca gcccgtcgag ctcgcccgca agcttgcgaa ggagatggag gagaaccaga
     8521 cggtctccat ctcccgcgtg tacgtgccca acgagtactg cgtgttcctg tcgccggacg
     8581 accgtgagca gttctcgggc tacgaggacg cgctgcgcaa agagctctct gactacctgc
     8641 tcgagcatgc gcgcaacgag aacttcgcgc tcgtgacgag gccgactgtc gatttccgca
     8701 ccgacgagcg tctgagcctg ggcgagttcg gaatccaggc gcagctcgtg cgcgcgcccg
     8761 aggacgaggc gggcgagccg gaggccggcg acttcgggca cacgatggtc tactcgcccg
     8821 atcgcgaggc gcgcaagctc gtcccgcctg gcggaaccgg ccgggcgatg ctcgtcggcg
     8881 caggacggcg caccgtgctc agcggcgagc gcgtggtgat gggccgcagc cgcgactccg
     8941 acatcgtgct cgacgatccg aacgtatcac gtcagcacgc cgagctgcgc aacaggggcg
     9001 aaggctgggt cgtgcgcgac ctcggctcta ccaatggcat aaaggtgaac ggcaggcggg
     9061 tagagcaggc tagtctgcag ccgggcgacg agatcacgct cggcctttcg cgcctcacct
     9121 tcgagcagga gtaatccgtg accgaccccg tggccgtcgc gctcaagttc ggctttctca
     9181 tcgtgctgta cctcttcctc ctgtgggtgg cccggagctc gatgcgtgac ctctcgcggc
     9241 acgaggacgc gagcatggtg atggagggcg ctccgcgcaa cgccgccgcg cgcagcgcgg
     9301 aacggcagcg cccggcgggc ccggacctgc gcgccggcgt gcggccgagg ctcgaggtgg
     9361 tggcggcgat gggccacgac cccggcgcgc gcttcgacat ttcgcagggg gcaacgctcg
     9421 gtcgctcgaa cgcgtcggac atcgaggtga acgacccgtt cgcctcgtcc gcgcacgcga
     9481 ggatcttccc gcggggccag ttcatgtaca tagaggacat ggggtccacg aacgggacct
     9541 acctcaacgg ccgccagctc cggcggccgg agcagctgaa ggtcgcggac accgtccgca
     9601 tcggcgacac cgagtaccgc tatcaggagt agcccgttgg gcctgcggat agtcgaggaa
     9661 gccgcgctca ccgacgtggg gcgccagaga aacgcgaacg aggacgccta ctacgagtcg
     9721 tcgccggtgt tcgccgtggc cgacggcatg ggcggagccc gcgccggcga ggttgcggcg
     9781 cggatcgccg tcgaggagtt cgatcaggtc gcggacgaga ccgcaagccc cgaggagcta
     9841 ctggccggca tcgcgcgctc ggccaacaag cgcatctacg agctcgcgca gcgcgacgag
     9901 acgcacgccg gcatgggcac cacgttcatc gcggcaatgg tctctggcaa cgaggtggcg
     9961 gtcgggcacg tgggagacag ccgcgcctac cgcttccgtg acgaggagct cgagcggctg
    10021 acgcaggatc actcgctcgt ggaggagctg cggcgacaag gcaagctcac cgaggcgcag
    10081 gcggaggctc acccgcagcg ctcgatcatc acgcgcgcgc tcggccccga gccggacgtg
    10141 gatgtcgaca ccttcagctt cccggcgcgc gacggtgacg tctacatgct ctgctccgat
    10201 gggctcaccg gcatggtgtc ggaggagcgg atcgcccaga tcctgcgcgt ccgttcgtcg
    10261 ctgcgtcagg ccgccgagga gctcgtgtcc gaggcgaacg cgcgcggggg acgcgacaac
    10321 atcacggtcg ttctcttcaa gctcggcggc gacgaggagc gcgaccagga ctccgacacg
    10381 ttgtcgggtc aggagacctc ggtggcgctc ggcgacggcg cgccggcggc cgacccggcg
    10441 catgagacga tggcgatgcc agccgctgag gcgaacgcgg cgcgcgcggc cgtcgcagag
    10501 cgtgaggcac cgcctccgga ggcgcccgcg gccgagccgc cacccgcgca gccggcgcga
    10561 aggggcaagc gctggctgaa ggccgccgtc ggtgtgctcg tggtcgctgc cgtgctcgcc
    10621 gggctctgga taggcagccg ccagttctgg ttcgtgggca ccgacaacca gggggcgatc
    10681 accctcttca gaggcctgcc gtacgagctt ccactgggcg tcaagctcta cacggaggag
    10741 taccagagca gcgtcccggc gctctcgttg ccgaagcttc agcggcagcg catcctcgac
    10801 caccagctac gcggccgctc ggacgcggtc gacctcgtcc gctcgctcga gcagacgcac
    10861 gcctcctgat gtccgcgcga actcgcgagt tcttcggcct catcccggtc gcgctcatgg
    10921 tcacagccgg cttcacggcc gtgctgatca cgcgctcgtc gcagatcagc accgcgacgc
    10981 tcacctacgg cgcggtgttc ctcgggctgt gcgtgttcgc gcacttcttc gtgcgcgcgc
    11041 ggctgcccga cgccgacccc tacatgttcc cgctcgccgc cctgctcgcc ggcttcgggc
    11101 tggtaatgat ctatcggatc aacccggacc tcgcccgcaa gcaggcgcag tggttcgtgt
    11161 tcgggctgat cgtgttctgc gcgacgatcg tgttcctgcg ggaccaccgt acgctcgagc
    11221 gctaccgcta cacgatcgcg gcggtgagca tcgcgctgct gctggcgccg cggctgccgg
    11281 ggatcggcgg cgcgacgaac ggcgcctacc tatccgtgag cttcggcgcg ttccagttcc
    11341 agccggccga gttcgcgaag atcggcgtga tcatcttcct cgcgagttac ctgcgcgaca
    11401 cgcgcgacgt gctcgtgcgc gggcgcttcc cgcacctcag tctcaagcac ttcgggccgc
    11461 tcctgatcat ctggggtgcg gccatgctga tgctgatctt catccgcgat ctgggcagct
    11521 cgctgatgtt cttcggcgcc ttcctcgcgc tgctctacgt cgccacctcg cggctgtcgt
    11581 tcgtggccgg cgggctggga ctgttcgcgc ttggggcgtg gttcctgggc agccacatcc
    11641 agcacgtcca ggaccgcgtg acggtctgga aggacccgtt caagcccggg ctcgtcgagc
    11701 acgagggcta ccagatcgcc cagggcttgt tcgcccaggc cgacggcggg ctgttcggca
    11761 aaggcttcgg ccaggcgctg ctgcagtcgc cgtacggcgg cacgctgctg ccggcgccgc
    11821 acacggacct catctacgcg gtgatcacgg atgagctcgg cctgttcggc gctgtcggcc
    11881 tgctggtcgt gtacctgctg ttcatcgacc gcggcttcaa gaccgcgctc gtcgccggcg
    11941 atgccttctc gaagctgctc gcgacggggc tcaccgcagt cgtcgcgatc caggtgttcg
    12001 tgatcgtggg tggcgtgacc aaggtaatcc cgctcaccgg cgtcacgctg ccgctcgtgt
    12061 cctacggcgg cagctccatc gtcgcgaacc tgctgctgct cgccctcctc ctgatcgtgt
    12121 ccgaccacgc gcggcgcgag ccgcacgctg ccgggggtgt ggtgcggtga acaggcagat
    12181 cacccaggtg ttcgggctga tgctgatcct ctttgcggtg ctcgtgggcg cgagctcgtg
    12241 gtggacggtg ttcggcgccg acggcctcaa ggacaacagc gcgaaccggc gcgacctgct
    12301 ccaagagcag cgcatcccgc gcgggttgat cctcaccgac aacggcgaca ctgcggcacg
    12361 cagcacgcgc ctcggcagcg gcaactcgct gcgctacgtg cgcacctatc ccaccggcaa
    12421 cctgttctcg cacgtcttcg gctactcgtt catccagctc gggcgcgccg gcctcgaaag
    12481 atccgagaac gacgctcttg cgggcaacca gaacgagttc tcatcgatag tggatgagct
    12541 gctgaaccgc ccgcaggagg gcttcgattt gcacaccacg ctcgacgcgg gcgcgcagcg
    12601 cctggcgcgg cagctgctcg ccggcaaggg gcgcgggtcg gtcgtcgcga tcgagccgaa
    12661 gaccggacgg gtgcgggtga tgctgagctt gccggactac gaccccaaca acatccccca
    12721 ggacttcaag cagctcaacc gcgatccgag ctcaccgctc ttcaaccggg ccacgcagtc
    12781 gggctatcca cccggctcga ccatgaaggt ggtgaccgcg tcggccgccc tcgacagcgg
    12841 caggtacacg ccgaactcgg tcgtgtccgg caagtcgccg aagatcgtgg gcggcgtgcc
    12901 cctctcgaac tgctgcacgg agggcagcgg caacttcggg gcattgactc tcaccgaggc
    12961 gctcgccaag tccgtgaaca cggtgtgggg cgaggtcggt gagaagctcg gtaagaacac
    13021 gatgttcgag tacatgcgcc gcttcggctt cgacagcaag ccaccgatcg acctgccgag
    13081 ggacgagctg cgctcgagcg gcgtctacgg ccgcggcggc aagctgctcg acgagcacga
    13141 cgccatcgac atcggccgcg tcgcgatcgg gcaggagcgc ctcaacgtga cgccgctcca
    13201 gatggccgag gtggccgcga cgatcgcgaa cggcggcgtt cgcatgcggc cccagctcgt
    13261 ggaccgcgtg gtggcgcgcg acgggcgcgt gaaggagcgg cgcaagccgg aagaggtcca
    13321 gcgcgtgatc aaggagtcca cagcgcgcga ggtcacgcag atgatgcagg gcgtcgtggc
    13381 caacggcacg gcgaccgcgg cgcagatccc cggcgtgcag gtggcgggca agaccggtac
    13441 ggccgaggtc gacaacggcg cggcgaacca ggcgtggttc atcgccttcg cgcccgcgaa
    13501 cgacccgaag gtcgcgatcg ccgtgacggt cgagagaact cagggagagg gcggcacggt
    13561 ggccgctccg atcgccaaac agatcatgca gtacctgctc gggaagggct gatggccgag
    13621 gtcgcagaga acacggtcgt cgacaaccgc taccggatcc tcaacaggat cggctcgggc
    13681 ggcatggccg acgtgtactg cgcggaggac acgcacctcg gccgccaggt ggcactgaaa
    13741 gtgctccacc ggcggttcgc gcaggacgac gagttcgttg agcggttccg gcgcgaggcc
    13801 tcggcggctg cggcgctgca gcacccgaac gtggtcaacg tgtacgaccg cgggaagcac
    13861 gacggcacct actacatcgc gatggagttc ctccaggggc gcacgctcaa ggagatcatc
    13921 caggcagagg cgccgctgtc gcaggagcgc gcgatcgagc tcgccatccc gatcctgcgg
    13981 gccgcgggct tcgcgcacaa gcgcggcgtg atccaccgcg acttcaagcc gcacaacgtc
    14041 atggtcagcc cgtccggtgc ggtgaaggtc acggacttcg gcatcgctcg cgccggcgcc
    14101 tccgagatga ccgagacagg ctcgatcatg ggcaccgccc actacctgtc gcccgagcag
    14161 gcgcagggcc acggcgtgac cgccgcttcg gacctctact cgatcggcgt gatgctctac
    14221 gagatgctgg cggggcaggt gcccttccag ggcgacagcg ccgtctcgat cgcgctgaag
    14281 cacctgagcg agccgccgcc gcgcctcgcg cagttcgcgc gggtgaaccc gcgactcgag
    14341 gcggtgctga accgcgcgct cgcgaagcag cccgagcagc gcttccagtc cgcggacgac
    14401 ttcatcgccg cgctcgagga ggcgcgcgcc gccatcgccg ccggcgacaa cggcgacgct
    14461 caggacaccg ccacctggac cgcagtcccg ccgccgccga tcgcgccgcc tccggcggag
    14521 cgcggacgtc gccgctggcc gtgggtcgtg cttgcagtgc tgcttgcggc cgggatcgcc
    14581 gccggtgtcg tcgcgctcgt gctcggcggc aatcgcgtca ccgtgccgaa ggtggtcggc
    14641 gagacgatcg agcaggcgag cccacagctc acccagaagg gcttccacgt ccgcacgacc
    14701 cggcggacca gcgagcagag cgcgggccta gtgctcgcac aggacccggc cggcggcgac
    14761 aaggccgaca agggctccac cgtgaacctc accgtctcga cagggcccgg caagaagacg
    14821 gtcccggacg tgcgcggcat gacgggccag gaggcgctca cggcgctgtc gaagcgaggc
    14881 ttcctcgtgg aggagcacca gcgccactcc acctccgtga aggacggctt tgcgatcggc
    14941 accgacccag cgaacggcac gagccaggag gtcggcagcc gcgttcagct cttcatcagc
    15001 tcgggcccgc gccaggtcgc ggtgccggac gtggtcggcc tcgacgtgga ctcggcgaag
    15061 tccaccctca gtgatcaggg tttcacctac acgaccacgc agcaggcctc ggaccagccg
    15121 gagggtcgtg tgctgtcgca gacgcccagc tccggcacga aggtcgacga gggcagccgc
    15181 gtgagcctcg tcgtgtcgaa ggggcccgac acggcagaca ttccggcggt cgtcggcctc
    15241 acggaacagc aggccaccga ccagatccgc gccgccggct tcgaggtgca ggtgaaggac
    15301 aagtccgtga acgaccccac gcaggacggc caggtcgtgc agcagcgccc gaagagcggc
    15361 aagctgaaga aggggcgcac cgtggtgatc tacgtgggcc gcgcggccca gcagccgggc
    15421 ggtacaggca ctccaccgat gggcggaggg accggctagt gcgggtggcc gtgctcgcgg
    15481 gcggtcgctc tagcgagcac gacgtgtcgc tcgcatccgc gacatccgtg gccgaggggc
    15541 tcgcgcaggc gggccacgag ccgctactcg tggagatctc gcgtgaggga gtctggagcc
    15601 tcgacggtga gccgctcgcg ctcactcccg gcaagcaggt ggccggcgcc gacatcgtct
    15661 tccccgtgct tcacggtccc tacggtgagg acggcacggt gcagggcctg ctcgagacga
    15721 tcggcactcc ctacgtgggc gccggcgtgc tgtcgtcggc tctctgcatg gacaaggtgg
    15781 tcttcaagga gctgatgtcg caggctggca tcccgcaggt cgactacgtg gcgttgcgcg
    15841 agggcgacga ctcgcggctc gcgtcgcgtc tcggcatgcc gctcttcgtg aagcccgcgc
    15901 gcctcggctc gtcggtgggg atctcgaagg tcagcagcgg cgaggcggag ctcgagcagg
    15961 cgctctcgct ggcatgggag cacgacccgg tggcgctcgt cgaggccttt tcaggcggca
    16021 tggaggtcga gtgctcggtg atcgggaacg acgcgcccga gacgtcgcag ccgggcgagg
    16081 tggtgccgca cgccgactgg tacgacttcg aggcgaagta ctcgccgggc gggatggagc
    16141 tggtcgtgcc gccccggctc gacgagcacg cgatcgaacg cgtccgcgcg ctcgccgtcg
    16201 aggtgtaccg gctggttggc tgctcgggct tcgcgcgcgt ggacttcttc gtcgagaacg
    16261 gccacaacgt gctcgtgaac gagctcaaca cgattccggg cttcacgccc acgagcggct
    16321 tcccgaagat gttcgaggcc tctggcgtgc cgtacccgga gctgctcgac cggctgctcg
    16381 ggctcgggat ggagcgcttc gagcgcgagc gccgctacgg gttctgagtc acgcgcagcg
    16441 tgacctcacg gatctcggca cggcccgagg gcggcagctt cgtgatccag atcaggtagt
    16501 tgcggtactg cttgccgccg gtgttcagct tcacgtgcga ggggccgttc accgtctggt
    16561 cgccgctgag cttggtccat ccagcgatcg tctggggcac ggggtcggcg ctcgtgccgt
    16621 acacggacat cgtccacgaa ccgggtcctg ttgtgagatc gagccagcgc gcgagcacgc
    16681 cgtggcccgc gtcgaggtag agcccgacac ccggcttccc gaggttgccg ccggtgtact
    16741 gctcggtggt ccaggtggtc gacgggttgt cgtcgatcac gttgcgcact gcttccgggt
    16801 gctcgcggcg gtcgccgagc gggtcgtagt cgtgcacgcc ggtgggggag aagctcacgg
    16861 tgcggagcgt cgcgctcgcc ttgctcggag gcgcgctgcc gggcgagccc ttcttcgtct
    16921 gcgtgagcag caggatggcc gccagcgcga tgccgccggc gagcagcgcg gtgatcacga
    16981 gcgcccgccg cggtgagcga aggcgccatg ggaggaagcg ctgcgtggtc ttgtccggca
    17041 gcgcgcgcag cacggtggtg gcctccccgg aggtctgccc cgcacgtgcc gcctcgatcg
    17101 cgagcgcctg ctcgaggtcg gtgagcatgt ccgccacgct gcggtagcgg ttgcgcgtct
    17161 ccttggccgt ggcggtgtcg acgatcgcgg cggtggcggc cgacacttcg gggcgcatgc
    17221 gctgcacgtc cggcatcggc tcgcgcacgt gcttcatcgc cacggccacg ggcgcgtcgg
    17281 ccctgtacgg cacgtcgccg gtgagcatct cgtagagcac cacgccgagc gagtagatgt
    17341 cggactgggg agtgaccggc tcgccgagag cctgctcggg cgacacgtag tcggtggtgc
    17401 cgagcacccg cccggccgcg gtgagcccgt cggcctcgag tgagcgtgcg atgccgaagt
    17461 cggtcacctt cgcgcggccg tctgcgtcga tcagcacgtt ctgcggcttc acgtcgcggt
    17521 gcacgagccg ctcagcgtgc gcggcctcga gcgcgcggcc gatctcaatg gcgtaggcga
    17581 tcgcctccgg aaccggcagc cgcgcgagcc gccggatgcg ctccttgagc gtctcgccgg
    17641 cgacgtactc gaacacgatg tagggctggc cgtcgtcctc gccggcgtcg atgacagtta
    17701 cgacgttcgg atgggagaag cgggccacgg cgcgggcctc gcgccggaag cgctcgatct
    17761 gatcgctgtc cgaggagatc tccgagtgga gcagcttgat cgccacccag cgctcgagcg
    17821 tctcatcgaa agcccggtag acggtggaca tgccaccgga cccgatcttc tcctccagcc
    17881 tgaagcggcc gctgagaagg gtgccgatca ctgtttctct attttggggt ccttgccggt
    17941 ccgagtccgc tacttcaccg atcccgcctg ctcctggtcg tggaacaacg agccggtggt
    18001 tcggaagctc atggtggaat tcggcacgga gctgtcgtgg tcctgggtca tgggcggact
    18061 cgcccgcgac tatacgcagg gtctcagcgg cggcgatgca ggtgcgagcg gcgacgacgc
    18121 ggtgtacgcc ttcctcacca ggcgctggtt gcaggccgcg gaatgggggc ggatgccgct
    18181 tgacccgctg atttggcacg aaggcccact cagctcgtcg tatccggcat gcatggccgt
    18241 gaaggcggcc gccgagcagt cgcccgacga cgacggctac cgctacctgc gcgccctccg
    18301 cgaggggatc ctctgcttta ggcgcaagct cgacaccacc gaggcgcttg tagaggaggc
    18361 acggcgcacc ggtctcgacg tccagcggtt ccgcatcgac ctcggctccc acgcgatcgt
    18421 ggaggcgttc ggcgccgatc tcgagctcac gcgcaacccg ccggaaaccg cacggcggga
    18481 ggggaaggtg gagcagcggc atggcggcga gcgtctgccg ttcccgagcg ccgtgttcat
    18541 cggcgaggac ggctccgagc acggcgtctt cggccgcgcg ccctacgaga cgtgggcggc
    18601 cgccgcggag gcagccggcg cgcggcgaac gcgcgaggag cagctttcgc ccttggcggc
    18661 cgtgcatcgg ttcggccgga tcgcgaccgc cgaggtcgag gctgtgtgcg acctgcccac
    18721 gccgagagcc gccgccaagc tgtgggagct ggcatcggac tggaagctga agccggtgcg
    18781 cgtgctcacc ggctggcttt gggagccggc ctagtcctcg tccgcggccg cgcgcactac
    18841 gagcaccgga cggtcagcgt gctgcagcac gccgtgggag gtgctgccga gcaccagcga
    18901 tttcagcccg ccctgccccc tcgagcccac cacgattgcg gccgagtcac gctcgcgagc
    18961 gagcctgacg agcgtctggg cgactgagac ctcgtcggcg acggccagcg cctcggcctc
    19021 gaagcccgcc tggcgcgcga tctccgctcc gcgctgagcc gtccgctgtg acgcctccgc
    19081 cagcgcctgg tcgacctcca aggacgcgcg cacgtcgatc ggcggcaacg ccgcgtccac
    19141 gccaagcgac gaggtcgtgt actcgagtgc cgtgcccggc tcccacaccg ccacgacgag
    19201 ggcgggcttg ccggtgagca cgcttccggc ctcgcgcaca gcgcgctccg cggcctcaga
    19261 gccgtcgaag ccgatcacaa ttggaccgga agccacgggg caagcctatg ggtttggagc
    19321 gaagccgcgc tcgcggcggc cggtgatccc cgcggacggg atctccacga ggccggcctc
    19381 ggaggtgacg gagccgatcc gcgccgcgcc tgggtggtgc ttcgcgagca gctgggtcgc
    19441 gcgttcggca tcggcctccg ggacgaccgc caccatgccg acgcccatgt tgaacgtctc
    19501 ccacaagtct gcgggtgcga tcccgccccg ctcggcgatc aggccgaaca ccggcggcgc
    19561 cggcagtggc tcgtcgaccc ggaagcccac gtcgtcgttc agtcgcagca ggttcagcat
    19621 gccgtcaccg gtgatgtggg cgaggccgcg cacggcggtt tccgagcgca gcagctccaa
    19681 caccgcacgc acgtagatca cggtcggctc gagcaatacc tcgccgacgc ttgcgccgcc
    19741 gagctcgcgc ggcgtctcgt cgagctcgct gagcacgctg cgcgcgagcg tgtacccgtt
    19801 cgagtggaga ccgctcgacg gcacgccgat cagcgcgtcc ccaggctcga tgtgatcgcc
    19861 agtgacgacc cggtcgagct gcaccgtgcc gaatgccgac gctgcgaggt cgaagccgaa
    19921 gggcgacgga tgtccgcgga tcagctctgg gagctgcgcg agctcgccgc ccggaatctc
    19981 gatgccggct tgctcggctc ccgctttcag gccctcgccg atctggcgca gcatctcggg
    20041 gttggccgcc tcgaccgcga tgtagtccac gagcgcgatc ggctcggcgc cgacgcacac
    20101 gaggtcgttc acgttcatgg cgatgcagtc gatgccgacc gtgtcgaagc gcccgagctg
    20161 ctcggcgagg atcaccttcg a
//
LOCUS       DASVXS010000029        15179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1352276, whole genome shotgun sequence.
ACCESSION   DASVXS010000029 DASVXS010000000
VERSION     DASVXS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15179
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352276"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VF752_01500"
     CDS             <1..592
                     /locus_tag="VF752_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8854246.1"
                     /translation="RPQQRARRADWRLRFVIFWAGMRGAVSLAAALAIPLETDAGAPF
                     PQRDLVIFLTFAVILVTLVAQGLTLPTVIRVLRVHDDGSAEQQEEVTARLAAADAALA
                     RLDELQEEDWTRDDTIERMRALFQYRQRRFAARVDGDDPEGIETRSIAFQRTQRSVLE
                     AQRAAIWDLQRRGVISNDVTHRLERELDLEDERLEI"
     gene            626..943
                     /locus_tag="VF752_01505"
     CDS             626..943
                     /locus_tag="VF752_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854247.1"
                     /translation="MLSDSLEERLEALAYWRQRRARLPWYRRSARREAERMVAAWEPR
                     VRSALVLDPRAPLPVRLNAAGLVLRTHAGRAVQRVRRAALMTVAAVSAAFLASVALIH
                     LIA"
     gene            1100..2449
                     /locus_tag="VF752_01510"
     CDS             1100..2449
                     /locus_tag="VF752_01510"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX8854248.1"
                     /translation="MAALTAAAVEAGRRVGPRGQIDAAAVRRDAGRELRRRAPRGSHA
                     HWEAPPDRPDPVDLLSASNDGRVPEVAAIRIGRMAASPSAFLSGSAGQIAFDLQDSPM
                     TGLTSWICGDAQLSNFGVCSSPEGELVCDISDFDESIVGPWEWDVKRLAASIVLAGRE
                     RGFRDADCRDAVETAVGSYRRRLGEMAARPLIDAHSARWQDGDVVGAALRHGSVPPRP
                     TVRRENGGWHFDESPPLLVRLGDEHTEKVLGGLDRYVSTLRSDLRRLVRGYALRDVAL
                     EVLGVGTAGTRNVLGLMVGNGDSDVLFLQITEARASQLRPYLASQPRRHEGRRVVDAQ
                     HAMQALPDPFLGWTAIDGREYYVRQLKDIKRSAAVGELQRLRDLRDYGHLAGGTLGMA
                     HARCFDATTLTAYLGSGARFAAAMAEFGVRYADQTERDHSELVRAIKRGRVEAELGI"
     gene            2611..4551
                     /locus_tag="VF752_01515"
     CDS             2611..4551
                     /locus_tag="VF752_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8854249.1"
                     /translation="MTSATVKERRADGKAPGPRLKRAPTELSAAAPSPFPPIADYAFL
                     SNCHTGALVAPDGAIGWLCVPSFDSPSIFATLLDREAGYFRVGPFGMNSTSQRIYEPG
                     TNTIITTWNTPSGWVVVRDALTMGPRHAEDTITPHTRPPADDDADHVLVRTVECIRGS
                     VEIEVVCEPVFDYGRTPAEWKMVGSDRLTADASGAGQTVRLRTDIPLGIEGSQARGRH
                     VLREGETLYTSLSWAEGLAGPATAVEANEALARTTRFWRGWLANARLPDHRFRQIVER
                     SALAIKGLTYMPTGATVAALTTSLPETPGGERNWDYRFTWIRDSTFTLQALHYLDLDW
                     EAQDFMQFVADLEPNDDGALQIMYGIDGRRDLTEWTVEELSGYAGARPVRVGNGAYDQ
                     RQNDVFGAVLDSILLHTRRSQRLPRRLWPIVESQAKCATDVWRNPDQGIWEARGKPRH
                     YVSSKLMCWVALDRAAQLAVIRGHDHLSAGWRETADEIKAEILDRGVDSRGVLKQHYE
                     TDALDASSLLAAMFGFLPGNDKRMQATVLAIADELTDHGYVLRYRTGETDDGMSGKEG
                     SFLICSFWLVSALARVGEMQRARDLIDKLLRVASPLGLYAEEFDADTGRHLGNFPQAF
                     SHLALLEAAAHIIVAERLAEFR"
     gene            complement(4556..4699)
                     /locus_tag="VF752_01520"
     CDS             complement(4556..4699)
                     /locus_tag="VF752_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854250.1"
                     /translation="MEPDAGTPPMAAPVAIYLRGTPSPRSKSVSQAFLDLRPGALIVV
                     ASD"
     gene            complement(4710..6047)
                     /locus_tag="VF752_01525"
     CDS             complement(4710..6047)
                     /locus_tag="VF752_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854251.1"
                     /translation="MAETRPGARQHRVLVASLLALAFVVGLVASLSLWANRQALNTDN
                     WVDTSSRLLADQKIQQAVGAYLVDELFSSVDVSAELQQVLPPRASALAGPAAAGLRDL
                     ADRAAPRLLDSPRAQTYWENANRTAHKELLAILNGGGKNVSTQNGEVVLNLHGLVNQL
                     ATGAGLSQSQVNAARGKVQGGAGAKAQKKLGVTLPPSSGELVILRSDQLSAAQDVADA
                     IRHLAFWLTGIFVALCALAVWFARGWRRVALRRVGWSFVALGVLVLFARRVGGNELVD
                     TLVKGQTVKPAAHDAWNIGTSLLYDIGIAAVAYGVAFVFAAWLAGPTVLAVGARRALA
                     SPLRYSLPAVYAVVAFLYLLLLLWGPTPALRKPIGILLFAVLIVLGVELLRRQTAREF
                     PDAQPGDTAARIRRSFAERRGRGRAATDERDGFSRGGVDEASDEASTPPARPA"
     gene            6218..6646
                     /locus_tag="VF752_01530"
     CDS             6218..6646
                     /locus_tag="VF752_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854252.1"
                     /translation="MTVQTPAAGETTAYAAYEEHGSGWVTFAGIMLMIVGTMNVISGI
                     AAIDNANFYVGNAQFFFSDLNTWGWIVLIIGAAQLLSALGIWARNQFARWLGVLFASL
                     NAIAQLLFLPAQPLFSLALFTIDILVIYGLIAYGRHEPAV"
     gene            complement(6650..7339)
                     /locus_tag="VF752_01535"
     CDS             complement(6650..7339)
                     /locus_tag="VF752_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854253.1"
                     /translation="MSIRVVIAEDSYLVRESLTQLLASAPDIEVVAVAEDTQSLRDAI
                     DREEPDVVLTDIRMPPALEREGIRVATHLRETRPEIGVVVLSLYAEPGFALELFDGGS
                     AGRAYLLKERVGNRGELTAAIRAVAEGGSVVDPKIVDTLIAERSRVADSRLADLTARE
                     REVLAEVATGKSNAAVASSLFLTKRAVEKHINAIFMKLGLRDSDDVSRRVKATLIFLA
                     EEGRAAPPPVV"
     gene            complement(7385..9562)
                     /locus_tag="VF752_01540"
     CDS             complement(7385..9562)
                     /locus_tag="VF752_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8854254.1"
                     /translation="MQTLTSLAPRRARSEIVDRPRLVRRLMDSAQATFAVVVAPAGYG
                     KSTLLRQWDARDSRPFAWLTLDESHDDPAVLCTSIARALDALEPAGGGVVKALPRRQP
                     PADALLSRLVRRAPFVLVLDDAHVLRDADAVDALRALARHIPAGSQLALGTRSEPPLP
                     IARLRANRELAEVRARELAMTTAEAASLLRNAGVSAGADVLDTLMRRTEGWPAGLYLA
                     ALSVGEQAGSTDAAARFGGDDMMVSEYIRDELLADLPAAAAEFLVHTAVLEELSGPAC
                     DLVLGRTGSERVLQGLARRNVMLVPLDRHGTTYRCHTMLKDTLDGELRRAAPRRLAEL
                     QHRASEWHEQNGDLGAALRHAVAAHDIERAATLLWRAAPQQLASGRHEDLAKALGQFT
                     EDQQRAHPALALAAAHLYAARGQYEAAQLWEQVAYEASDRSPEPALDAGFAILSATLA
                     RGGASGMVRDAGRAYELAQGSSAWRSICCLLEGVGLYLSGETARAEERLEEGVRRSAA
                     SAPALQAQCLAQLSLIAIEREEWDEGAALIGRAAAQLDRHALRDEPASAIVFAAAAAL
                     RAHLGRTDDARRDARHAVRLLRCKAAFQRWYEVQAKLALARTELRLGNVAEAGRLLDE
                     AARAAAGAGKAPGLESWVASVGASVASVSGSSGERASPLTAAELRILGFLPTHLSFRE
                     IAGRLYVSANTVKTQAHSVYRKLDASSRSEAVAHAAKLGLIDA"
     gene            9845..10342
                     /locus_tag="VF752_01545"
     CDS             9845..10342
                     /locus_tag="VF752_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX8854255.1"
                     /translation="MADLVAIGYNDETTAAAAADEVRRLSADLIIEPEAVAVISRDAD
                     GKYHVTTDHHPVGGGASWGMFWGLLFGLLFFIPVFGMAVGAGLGALFGKIEKTGIDKQ
                     FQEQVRDMVKPGTSALFVVVDKVTPDKAVEALSRFGGTVLKSSLSKDAENELQEALHG
                     APVSA"
     gene            10390..10809
                     /locus_tag="VF752_01550"
     CDS             10390..10809
                     /locus_tag="VF752_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854256.1"
                     /translation="MTAPGGTLKDRPADGRVGVLVVDDQLLFREAAREVIAATPGFEL
                     VGEAVSGEQALRAADELCPDLILLDVRMPGMGGVEAAARLHKAHPDQLVVLISTEQPD
                     VVERRVKTHDAAAIVPKHCFGAALLRSLWRMYGSAPG"
     gene            10973..12685
                     /locus_tag="VF752_01555"
     CDS             10973..12685
                     /locus_tag="VF752_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8854257.1"
                     /translation="MAANAADEDRALSMVTRGLAIALPIGLGAFRLNRNPGDRFAKLL
                     VAAGLVFSLTSLAEADGAVPYSIGRALVWVVEPVVVYLLLAFPTGRITTAVERRLVWA
                     VVLLAGLLYLPTALIEEGFPTPNPWASCGEQCPHNALALGSAPEVMDVVRPLREVLSV
                     LLFGAVVVVLWRRTRRVRPMLQHALVPVVAIALGRVVALAAYDVLRATDGQSDATGVI
                     GWLYLFSLPAITVCFAIGLVNQRVFAAGALERLALELGPHANAANLRLAMAEQLDDPE
                     LRVVYWLPGDPGRWIDETGWPVKPPQQENGQVVTKVNDGGQPVAAVVHDAALAQDPKF
                     VEAASVYALATLENERLIGRLHGSLRELAQSRARLVAVADEERRRIERDLHDGAQQRL
                     VALRIKLELIAERLQELSPHDAAALRELEDVVETTIEQVRSFARGIYPALLAERGLDE
                     ALRALARDCPLPTAVYTEDVERYSHEIEATVYFTVAEALQNATKHARGVTAATIHVRG
                     GERLEFDVHDDGEGFAPETAFTGVGLVNFRDRLEAVGGEASVRSTPNYGTTVSGSIPL
                     EVGT"
     gene            12682..13731
                     /locus_tag="VF752_01560"
     CDS             12682..13731
                     /locus_tag="VF752_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8854258.1"
                     /translation="MNWPARRRSYDADGLTPALLENLADAVVACDAEGSIVLLNRVAR
                     DLLAPAGAPVVEVPLERALRGEDVRDEQLELPSADGPSVFLNVSGGPLRNRRGSIDGA
                     VLVMQDCTARVAMENELRLQSVIAERMAEGVVLVRASDGVIVYANERWERMFGAGRGE
                     LLGQHVSVVNAPTEKPPEERAQEIIGALERDGVWSGEIRNIRRDGTLIWTVAHISTFE
                     HRDHGTVWISVQSDVTPRKAAQDAMREAEERFRGVFEHSPFGIALLGQDLRLRETNRA
                     FGEITGYSPDELVGESLPEFSVVFNGHQSNHRVVTRLATKSGETVPVAITGTAVHGAD
                     GDPLYGIAVVQPIDA"
     gene            13800..14030
                     /locus_tag="VF752_01565"
     CDS             13800..14030
                     /locus_tag="VF752_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854259.1"
                     /translation="MEGSQETGQVTGVKDKDYNIIWFTEQSLSNALRLETYIQDAQRE
                     GDSELADFFRRAQEASRKGGQQGKELLRARLG"
     gene            complement(14052..>15179)
                     /locus_tag="VF752_01570"
     CDS             complement(14052..>15179)
                     /locus_tag="VF752_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854260.1"
                     /translation="GRTREALAVVERGREVVSGFSRPRDWLSVMIAELKWQLGEWDEA
                     EAWMPPSERRYVATTTMYVLLQRATFALGRGDHATARRYLEEIEEFVAESTEPQFLGT
                     WGALRASLSLREGDIDAARAAVDEALDRIEFCSEDANRIVRLSAIGVAIEAAAIERAR
                     DLGEDTALAQFRAEAMVSRLRAGAEGDRPVERAWLAMAEAEGTRVDGIPEPSCWAEAA
                     SAWDRLEWPYEAALARWRGAEAHVQAGDREQAALWAGNALRTARELGAGWLEREVEGL
                     IARARLRLEDEAAAEEPEPGEVEEEEPFGLTPRERQVLALVADGRTNREIGDTLFMAE
                     KTASVHVSRILSKLGVRSRTEAAAVAHRLGLDSREPAQRDA"
BASE COUNT         2324 a         5343 c         5268 g         2244 t
ORIGIN      
        1 ccgaccccag cagcgcgcgc ggcgcgccga ctggcgcctt cgcttcgtga tcttctgggc
       61 ggggatgcgc ggcgcggtgt cgctcgccgc tgcgctcgcg atcccgctcg agaccgacgc
      121 gggcgcaccg ttcccgcagc gcgacctcgt catcttcctg acgttcgcgg tgatcctcgt
      181 aacgctcgtg gcgcaggggc tcacgctgcc cacggtgatc cgtgtgctcc gggtgcacga
      241 cgacggctcc gccgagcagc aggaggaggt gaccgcgagg ctcgcggcgg cagacgcagc
      301 gctcgcgcgc ctcgacgagc tgcaggaaga ggattggacc cgtgacgaca cgatcgagcg
      361 gatgcgcgcg ctgttccagt accggcagcg ccgcttcgcc gcgcgcgtcg acggggacga
      421 tcccgagggc atcgaaacgc gctcgatcgc ctttcagcgc acccagcgct cggtgctcga
      481 ggcgcagcgg gcggcgatct gggacctgca gcgccgcggc gtgatctcca acgacgtgac
      541 gcaccggctc gagcgggagc tcgacctcga ggacgagcgg ctcgagatct agtcggtgtt
      601 gcgtgttggt gcctagactg acgccgtgct gagcgactcg ctcgaggaac ggctcgaggc
      661 gctcgcctac tggcgccagc gccgggcccg actgccgtgg taccggcgga gcgcgcgccg
      721 cgaggcggag cggatggtgg ccgcgtggga gccgcgcgtt cggagcgcgc tcgtgctgga
      781 tccgcgggcg ccgctgccgg ttcggctgaa cgccgccggc ctggtgctgc ggacgcacgc
      841 gggccgcgcg gtgcagcgtg tgcggcgcgc ggcgctcatg acggtcgcgg ccgtcagtgc
      901 ggctttcctg gcatccgtcg cactcatcca tttgatcgcc tgagcggtct ggcgagccct
      961 cgacggggcg cacgcaccag caacatcgtg cggcgcgcac cattgcgacg cgcggcggcg
     1021 gtcggtcaga gtgccagcga tggcggtctt cgccggctcc tcaacgtccc cagccgctga
     1081 ggccgaaccg gccgccacga tggctgcact caccgccgcc gcggtcgagg cgggccgccg
     1141 cgtaggcccg cgcggccaaa tcgacgcggc cgccgtgcgg cgtgacgccg gccgcgagct
     1201 ccgtcgccgc gcgccccgcg gaagccacgc gcactgggag gcgccgccgg accggccaga
     1261 tccggtcgat ctcctgagcg cctcgaacga cggtcgcgtg cccgaagtcg cggctatcag
     1321 gatcggccgc atggcggcct cgccttccgc gttcctctcc ggcagcgccg gacagatagc
     1381 gttcgatctc caggactcgc cgatgaccgg cctcacgagc tggatatgcg gcgacgcgca
     1441 gctgtcgaac ttcggcgtgt gctcgtcgcc ggagggcgag ctggtgtgcg acatcagcga
     1501 cttcgacgaa tcgatcgtcg gcccctggga gtgggacgtg aagcggcttg cggccagcat
     1561 cgtgctggcc ggccgggagc gcggcttccg tgacgccgac tgccgcgacg cggtcgagac
     1621 ggcagtgggc tcctaccggc ggcggctggg cgagatggcc gcgcgcccat tgatcgacgc
     1681 gcactccgcg cgctggcagg acggcgacgt cgtaggcgcg gcgttgcggc atggcagcgt
     1741 acccccgcgc cccaccgtgc gtcgcgagaa cggcggctgg cacttcgatg agtcgcctcc
     1801 gcttctcgtg aggctcggcg acgagcacac ggagaaggtg ctcggtggtc tcgaccgcta
     1861 cgtctccacc ctgcgcagcg acctgcgccg cctcgttcgc ggctacgcgc tgcgcgacgt
     1921 ggccctcgag gtcttgggag tcggaaccgc cggcacccgc aatgtcttgg gcctgatggt
     1981 cggcaacggc gacagcgatg tgctcttcct gcagatcacg gaggcgcgcg cctcgcagct
     2041 tcgcccctat ctcgcctcgc agccgcggcg ccacgagggc cgccgcgtgg tggacgctca
     2101 gcacgccatg caggcgctgc ccgacccgtt cctcggctgg accgcgatcg acggtcgtga
     2161 gtactacgtg cgccagctca aggacatcaa gcgctcagcg gccgtgggcg agttgcagcg
     2221 cctgcgcgac ctccgcgact acgggcacct ggccggcggg accctcggca tggcacacgc
     2281 gcgctgcttc gacgcgacca cgctcacggc gtacctcggc tccggcgcgc gctttgctgc
     2341 ggccatggcg gagttcggtg tgcgctacgc ggaccagacc gagcgcgacc actccgagct
     2401 cgtgagggcc atcaagcgcg gacgcgtgga ggccgagctt ggaatctgac gggccgattc
     2461 acctacttcg ggtgatgcgc cctccgctcg ggttgttgac gatgcacgag acccggggcg
     2521 cggtgcacct ccgccgggcc ccagcggcgc tgccggcact gccccctcgt catgccggca
     2581 gcgccaaacc ttttcgcgga gaggaaggtc atgactagtg caacggtgaa agaacggcgc
     2641 gcagatggga aagcgccagg accccgcctc aagcgcgccc ccaccgagct gagcgcggcg
     2701 gcgccctcgc cgttcccgcc gatcgcggac tatgccttcc tctcgaactg ccatacgggt
     2761 gcgctcgtag cgcccgatgg ggcgatcggg tggctctgcg taccgagctt cgactcaccg
     2821 agcatcttcg cgaccctcct cgaccgcgag gccggctact tccgggtcgg gccgttcggg
     2881 atgaactcca cctcgcagcg catctacgag ccgggtacga acaccatcat caccacgtgg
     2941 aacacgccga gcggctgggt cgttgtgcgc gacgcgttga cgatggggcc acgccacgcg
     3001 gaggacacga tcaccccgca tacgcggccg cccgccgacg acgatgccga ccacgttctc
     3061 gtgcgcaccg tcgagtgcat ccgtgggagt gtggagatcg aggtggtgtg cgagccggtc
     3121 ttcgactatg gccgcacacc ggccgagtgg aagatggtag gaagcgaccg cctcacggcg
     3181 gacgcgagcg gggccggcca gaccgtgcgg ctgcgaacgg acatcccgct ggggatcgag
     3241 ggcagccagg cgaggggacg acacgtgctc cgcgagggcg agacgctgta cacctcgctg
     3301 tcgtgggccg agggactcgc aggcccggcg accgcagtcg aggcaaacga ggcgctcgcc
     3361 cggaccaccc gcttctggcg cggctggctc gcaaacgcgc gcctccccga ccaccggttc
     3421 cgccagatcg tggagcgctc agcgctcgcg atcaaggggc tcacctacat gccaaccggc
     3481 gccacggtgg cggcgctcac cacgtcgctg cccgagacgc ctggcggtga gcgcaactgg
     3541 gactaccgct tcacctggat acgcgactcg accttcacgc tacaggcgct ccactatctg
     3601 gacctcgact gggaggccca ggatttcatg caattcgtcg ccgacctcga gccgaacgac
     3661 gacggcgcgc tccagatcat gtacgggatc gacggtcgcc gcgacctcac cgaatggacg
     3721 gtggaggagc tctccggcta cgcgggcgct cggccggtgc gcgtgggaaa cggtgcgtac
     3781 gaccagcgcc agaacgacgt gttcggagcc gtgctcgact ccatcctcct gcacacgcga
     3841 cgcagccagc gactgcctag gcggctgtgg ccgatcgtgg agtcccaggc gaagtgcgcc
     3901 acggacgtgt ggcggaaccc ggaccagggg atctgggaag cccgcggcaa gccacggcac
     3961 tacgtctcct cgaagctcat gtgctgggtc gcgctagatc gcgcggcgca actcgcggtc
     4021 atccgcggcc acgaccactt gagcgccggc tggcgcgaga ccgccgacga gatcaaagcc
     4081 gagatcctcg accggggcgt cgacagccgc ggcgtgttga agcagcacta cgagaccgac
     4141 gcgctcgacg cctccagcct cctggccgca atgttcgggt tcctgcccgg caacgacaag
     4201 cgcatgcaag cgaccgtgct ggcgatagcc gatgagctca cggaccacgg ctacgtgctg
     4261 cgctatcgga ccggcgagac ggacgatggc atgtccggta aagagggctc gttcctgatc
     4321 tgctcgttct ggctggtgtc agcgctcgcg cgggtgggcg agatgcagcg tgcacgcgac
     4381 ctgatcgaca agctcctgcg cgttgcctca ccgctcggcc tctacgcgga ggagttcgac
     4441 gccgacacgg gccggcacct gggcaacttt ccgcaggcgt tctcacacct cgccctgctc
     4501 gaggccgcgg cgcacatcat cgtcgccgag cggctcgcgg agttccgctg agtcctcagt
     4561 ccgaggcgac gacgatgagc gcgcccggac gaaggtcgag gaaggcctgc gaaacgctct
     4621 tggagcgcgg gctcggagtg cccctcaggt agatcgcgac cggcgcggcc atcggcggag
     4681 tgccagcgtc cggctccatg ccgagcgact catgcgggtc tggcgggggg agttgaggcc
     4741 tcatcagagg cttcatctac cccaccgcgg gaaaatccat ccctctcgtc ggtagccgcc
     4801 cggccgcgtc cccgccgctc ggcgaagctc cgcctgatgc gcgcggccgt gtcgcccggc
     4861 tgtgcgtcag gaaactcgcg cgcggtctgc cgccgcaaca gctccacgcc cagcacgatc
     4921 aatacggcga acaggaggat cccgatcggc ttacgaagcg ccggcgtggg tccccagagc
     4981 aggagcagca ggtacaggaa ggcgaccacc gcgtagacgg ccgggaggct gtagcgaagt
     5041 ggcgacgcga gggctcggcg ggcacctacg gcgagcaccg tcggaccagc cagccaggcg
     5101 gcgaagacga acgccacgcc gtatgcgacc gctgcgatac cgatgtcgta gagcagggac
     5161 gtgccgatgt tccaagcgtc gtgcgctgcc ggcttcaccg tctgcccctt cacgagcgta
     5221 tcgacgagct cgttgccgcc cacccgacgc gcgaacagga ccagcacgcc cagcgcgacg
     5281 aaggaccagc ccacgcggcg cagggcgacg cggcgccaac cacgcgcgaa ccacacagcg
     5341 agcgcacaca aggcgacgaa gattcccgtg agccagaagg cgagatggcg gatcgcgtcg
     5401 gccacgtcct gagcagccga aagctggtcc gagcggagga tcacgagctc gccgctcgac
     5461 ggcggcagcg tgacgccgag cttcttttgc gccttcgcac ccgctccgcc ctgaaccttg
     5521 ccccgggcag cgttcacctg gctctgtgag agcccggcgc ccgtggcgag ctggttcacg
     5581 agcccgtgga ggttgagcac cacctcgccg ttctgggtcg agacgttctt gccgcctccg
     5641 ttcaggatcg caagcagctc cttgtgagcg gtgcggttgg cgttctccca gtaggtctga
     5701 gcgcgaggcg agtcgagcag gcgcggcgcg gcgcgatcgg cgagatcgcg cagcccggcc
     5761 gcggccggac ccgccagagc agacgcgcgc gggggaagca cctgctgaag ctccgccgag
     5821 acgtcgaccg agctgaacag ctcgtccacg aggtaggcgc ccaccgcctg ctggatcttc
     5881 tggtcggcca ggagccggct gctcgtgtcg acccagttgt ccgtgttgag cgcctggcgg
     5941 ttcgcccaga gagacaggct cgcgaccaac ccgaccacaa aggcgagcgc gagcaggctc
     6001 gcaacgagca cgcggtgctg gcgcgctccg gggcgggtct cagccatgcc gagtcacgtg
     6061 ttgaagatcg ccccagcggg cagccggggc atcgcccaaa agggatgaat cggcgcgtgt
     6121 gcgcgcctcc ccccgagcgt ggtgggcgca cccttgagcg cgatctggac gggtgcgagc
     6181 gttgccgcaa cgccgtcctc aaccacagga ggagctcatg acggtccaaa ctccagccgc
     6241 gggcgaaacg accgcctacg ccgcgtacga ggagcacggc agcggctggg tcacgttcgc
     6301 cggcatcatg ctgatgatcg tcggaacgat gaacgtgatc tccggcattg ccgcgatcga
     6361 caacgccaat ttctacgtcg ggaacgcgca gttcttcttc agcgacctga acacgtgggg
     6421 atggatcgtg ctgatcatcg gcgcggcgca gctgctgtca gcgctcggga tatgggcgcg
     6481 caaccagttc gcccgctggc tcggtgttct attcgcgagc ctgaacgcga tcgcgcaact
     6541 actgtttctg ccggcccagc cactcttctc gctggcgctg ttcaccatcg acatcctggt
     6601 gatctacggc ctcattgcct acggccgcca cgagcctgcc gtgtagccgt cacaccacgg
     6661 gtggaggcgc cgcgcgccct tcctcggcca ggaagatcag cgtcgccttc acccggcggc
     6721 tcacgtcgtc cgagtcgcgc aagccgagct tcatgaagat cgcgttgatg tgcttctcga
     6781 ccgcgcgctt cgtgaggaag agcgacgatg ccaccgcggc gttgctcttg cccgtcgcca
     6841 cctcggcgag cacttcgcgc tcgcgtgccg tgaggtcggc gagccgcgag tcggccactc
     6901 gcgaccgctc cgcgatgagt gtgtccacga tcttcgggtc gacaaccgag ccgccctcgg
     6961 cgacggcgcg gatcgccgcc gtcagctcgc cgcggttccc aacccgctcc ttgagcaggt
     7021 aggcgcggcc ggctgagccg ccgtcgaaga gctcgagcgc gaagccgggc tccgcgtaca
     7081 ggctcaaaac caccaccccg atctcgggcc gagtctcgcg cagatgcgtg gctaccctga
     7141 tgccctctcg ttccagcgcc ggcggcattc ggatatcggt cagcacaacg tcaggttcct
     7201 cgcggtcgat cgcatcgcgc agactttggg tgtcctccgc gaccgccacc acctcgatgt
     7261 ccggagcgga ggcgagcagc tgcgtgaggc tctcacgcac caagtagctg tcctccgcaa
     7321 tcaccacgcg gatactcatc tcccccgagc gtacgcgccg ccccgaacgg cagtcaacca
     7381 ccggtcaggc gtcgatcagg cccagtttcg ccgcgtgcgc gaccgcctcc gagcgcgagg
     7441 acgcgtcgag cttgcggtac acggaatgcg cttgcgtctt cacggtgttc gccgacacgt
     7501 agagccgtcc cgcgatctcg cggaaggaca ggtgtgtcgg gaggaaaccg agtatgcgga
     7561 gctcggccgc cgtgagtggt gaggctcgct cgccgctcga gccggacact gacgcgacgc
     7621 ttgccccgac cgaagccacc cagctctcga gccccggagc tttccccgcg cccgcagccg
     7681 cgcgcgcggc ttcgtcgagc agcctgccgg cttcggcaac gttcccgagg cggagctccg
     7741 tccgggccaa cgcgagcttc gcctgcacct cgtaccagcg ctggaacgcc gccttgcagc
     7801 ggaggaggcg gacggcgtgc ctggcgtcac ggcgagcgtc gtcggtgcgg cccagatgcg
     7861 cccgcagggc ggcggcggcg gcaaacacga tcgcggaagc cggctcatcc cgcagggcgt
     7921 gccggtcgag ctgcgccgcg gcgcggccga tcaatgctgc tccctcgtcc cactcctcgc
     7981 gctcgatcgc gatgagagac agctgcgcga ggcactgtgc ctgcagtgcg ggtgccgacg
     8041 cggcgctccg gcgcactccc tcttcgaggc gctcctccgc ccgcgcggtc tcgcctgaca
     8101 gatacaggcc gacaccctcg agcaggcagc agatcgaacg ccacgcgctc gagccctgcg
     8161 cgagctcata ggcgcgaccg gcatcgcgaa ccatgccgga cgcgccccct cgcgcgagcg
     8221 tcgcgctcag gatcgcgaat cccgcgtcga gcgccggctc cggcgatcgg tcggaggcct
     8281 cgtacgcgac ctgctcccag agttgcgccg cctcgtattg gccgcgcgcg gcatacaggt
     8341 gggcggccgc gagcgcgagg gccggatggg ctcgttgctg atcctcggtg aactgaccga
     8401 gggccttcgc gaggtcctcg tggcggccgc tcgcaagctg ttgcggagca gcgcgccaca
     8461 gcagtgttgc cgcccgctcg atgtcatggg ccgccacggc gtgccgaagc gccgccccca
     8521 gatcgccgtt ctgctcgtgc cattcgctgg cacggtgctg gagctcggcc aggcggcgcg
     8581 gcgcggcgcg gcgcagctcg ccgtcgagcg tgtccttcaa catcgtgtgg cagcggtacg
     8641 tcgtcccgtg cctgtcgagc gggacgagca tcacgttgcg ccgggcgagc ccctgcagca
     8701 cacgctccga cccagtgcgc ccgagcacca gatcgcatgc cggtcccgaa agctcttcga
     8761 gtacggcggt gtggaccagg aactctgccg ccgcggccgg aaggtctgcg agcagctcgt
     8821 cgcggatgta ctcggacacc atcatgtcgt cgccgccgaa gcgcgccgcc gcgtcggtgg
     8881 agcccgcctg ttcgccgacg gataacgccg ccagatagag gcccgcgggc cagccctcgg
     8941 tgcggcgcat caacgtgtcc agcacatcgg cgccggccga tacaccggcg ttccgtagca
     9001 gcgaggctgc ctcggcggtc gtcatcgcga gctcgcgggc ccgcacctcg gcaagctcgc
     9061 ggttcgcgcg cagtcgtgcg atcggcagcg gcggctcgga ccgcgtgccc agtgcgagct
     9121 gcgatccagc aggtatgtgc cgcgccaggg cgcggagagc atctaccgca tcggcatcgc
     9181 ggagcacgtg cgcgtcgtcg agcacgagga cgaacggcgc gcgccgcacg aggcgcgaca
     9241 gcagggcatc ggccggcggc tgacgccgcg gcagtgcctt caccacacct ccgcccgccg
     9301 gctcgagcgc gtcgagggcg cgcgcgatcg acgtgcagag caccgcagga tcgtcatgcg
     9361 actcgtcgag cgtcagccat gcgaacggcc gcgaatcgcg agcatcccac tgcctgagca
     9421 aagtgctctt gccgtagccc gcgggggcga cgaccaccgc aaacgtcgcc tgcgccgagt
     9481 ccatcagccg gcgcacgagg cgcgggcgat cgacgatctc gctgcgggcg cgccgaggcg
     9541 ccagggatgt gagtgtctgc accgcgacag cgcttgaagc gcgcaccgcg cctgatcgtg
     9601 atcgttcgcc catgtgtgcc gtcctctgct gcgactccga ccctctcggg agacgagccg
     9661 gtgggcaagg aggctcacct gcctgtgctg atggggcctg caccacccgg cgcaagggtg
     9721 cggcgagaca cagcgttcac cccgttcgga tgatgccgct ttgccggtac tggcctaggt
     9781 tcggaggtcc ggtgccgcaa cgccgtccac ggcatcggct cgtcagccac accaaggagg
     9841 acacatggca gatctcgtag cgattggcta caacgacgag actacggccg cagcagcagc
     9901 ggatgaagtc aggcgcctct cggccgatct gatcatcgaa cccgaggcgg tcgccgtgat
     9961 ctcgcgcgac gccgacggga agtaccacgt gaccaccgac catcacccag tcggcggtgg
    10021 cgcgtcgtgg ggcatgttct ggggtctgct tttcggcctt ctcttcttca tcccggtgtt
    10081 cggcatggcc gtcggggcgg gcctcggcgc gctcttcggg aagatcgaga agacgggcat
    10141 cgacaagcag ttccaagagc aggtccgcga catggtgaag cccgggacat ccgcgctgtt
    10201 cgtggtcgtc gacaaggtca cgcccgacaa ggcggtcgaa gcgctgagcc gcttcggcgg
    10261 caccgttctg aagtcctcgc tctcgaagga tgccgagaac gagcttcagg aagctctcca
    10321 cggcgcgccc gtgagcgctt gacgggacag actgctccgg ctccatgcgg ggagccgggg
    10381 cccgcggcga tgactgcacc tggcggaacc ctgaaagacc ggcccgcgga cggcagggtc
    10441 ggcgtcctcg tggtcgatga ccagctgctc ttccgcgaag ccgcgcgcga ggtgatcgcc
    10501 gcgactccgg gattcgaact ggtcggcgag gcggtgtcag gcgagcaggc gctccgcgcc
    10561 gccgacgagc tctgccccga cctgatcctg ctcgacgtgc ggatgccggg catgggcggt
    10621 gtggaggccg cggcgcgcct gcacaaggct caccccgatc agctcgtggt gctgatctcc
    10681 acggagcagc ccgacgtcgt ggagcgacgc gtgaagacgc acgacgcggc ggcgatcgtc
    10741 cccaaacact gcttcggcgc ggcactcctg cgcagtctct ggcgcatgta cggatcagct
    10801 cccggataac gcccgaattt gccaagggcg gcgcctccgg cggtacgatc tgtgtagcgc
    10861 catcatggcc gatatcgccg agaccacgtc gacgcggctg cgccccagaa cgtcgactgg
    10921 gaccgctgtt gcactaaccg gagcccttgt cacgaccgca gcgttgtggt tcgtggccgc
    10981 gaacgctgcc gacgaggatc gtgcgctttc gatggtcacg cgcggcctcg cgatcgcgct
    11041 gccgatcggg ctgggagcat tccggctcaa ccgcaaccca ggcgatcgct tcgccaagtt
    11101 gctggtggca gccgggcttg tgttctcgct gacgagtctc gccgaggccg atggcgctgt
    11161 gccgtacagc atcggccggg cgctcgtgtg ggtcgtcgag ccagtcgtgg tctacctgct
    11221 gctcgccttc cccaccgggc gcatcacgac cgctgttgag cggcgtctcg tctgggcagt
    11281 cgtcctcctg gccggcctcc tctacttgcc cacagcgctg atcgaggagg ggttcccgac
    11341 gcccaatccg tgggccagct gcggcgagca gtgcccgcac aatgcactcg cgctcggctc
    11401 tgctccggaa gtgatggacg tcgtccggcc tctccgcgag gtgctcagcg tgctgctttt
    11461 cggggccgtc gtggtcgtcc tatggcggcg tacacgccgc gtccggccga tgctccagca
    11521 cgcgctcgtc ccggtggtgg cgatcgcact gggccgtgtg gtggcgctcg ccgcatacga
    11581 cgtgcttcgc gcgactgacg ggcagtccga cgcgacgggg gtgatcggat ggctgtacct
    11641 gttctcgctc ccggcgatca ccgtctgctt cgcgatcggc ctggtgaacc agcgtgtctt
    11701 cgctgcggga gcgctcgagc gcctagccct cgagcttggg ccgcacgcga acgccgccaa
    11761 cctgcgactg gcgatggcgg aacagctcga cgatccggag ctgcgtgtcg tgtactggct
    11821 gcccggcgat ccgggccgct ggatcgacga gaccggctgg cccgtgaagc cgccgcagca
    11881 ggaaaacggg caggtggtca ccaaggtcaa cgacggcggc caaccggtcg cggcggtggt
    11941 acacgacgct gcgcttgcgc aagacccgaa gttcgtggag gcggcctcgg tatacgcgct
    12001 ggctacgctc gagaacgagc gcctcatcgg ccggctgcac ggctcgctcc gcgagctcgc
    12061 gcaatcacgg gcccggctcg tcgccgtggc cgacgaggaa cgccgcagga tcgaacgtga
    12121 cttgcacgac ggcgcgcagc agcgcctcgt cgcccttcgg atcaagctcg agctgatcgc
    12181 tgagcggctc caggagctgt ccccgcatga tgctgcagcc ttgcgggagc tggaggacgt
    12241 ggtggagacg acgatcgagc aggtgcgctc gttcgcacgc gggatctacc cggcgctgct
    12301 tgcggagcgg gggctcgacg aggcgctgcg cgcgctcgcg cgcgactgcc cgctgccgac
    12361 ggccgtgtac accgaggacg tggagcgcta ctcccatgag atcgaggcga ccgtgtactt
    12421 caccgtggcc gaggcgctgc agaatgccac gaagcacgcg cgcggcgtca ccgccgcgac
    12481 catacatgtg cgaggcggcg agcgcctcga gttcgacgtg cacgacgacg gcgagggctt
    12541 cgcgcccgag accgcgttca ccggcgtcgg cctcgtcaac ttccgcgacc ggctcgaggc
    12601 cgtcgggggc gaggcgagcg ttcgctcgac cccgaactac ggcaccacgg tctccggcag
    12661 catcccgctc gaggtgggca cgtgaactgg ccggcccgga ggcgctcgta cgacgcggac
    12721 ggcctcaccc cggctctcct cgagaacctg gcagacgccg tggtcgcctg cgacgcggag
    12781 ggcagcatcg tcctgctcaa ccgcgtggca cgcgacctgc tggcgccggc cggggcgccc
    12841 gtcgtcgagg tcccgctcga acgcgccctc cgcggggagg acgttcgcga tgagcagctg
    12901 gagctgccat ccgccgatgg accctccgtc ttcctcaacg tgagcggagg gccgcttcgc
    12961 aacaggcgcg gttccatcga cggtgcagtg ctggtcatgc aggactgcac cgcgcgcgtc
    13021 gcgatggaga acgagcttcg cctccagagc gtgatcgccg agaggatggc ggagggggtc
    13081 gtgcttgttc gcgcgagcga cggcgtgatc gtctatgcga acgagcggtg ggagcggatg
    13141 ttcggcgccg ggcgcggcga gctgctcggg cagcacgtct cggtcgtgaa cgcgccgacc
    13201 gagaagccgc ccgaggagcg agcgcaggag atcatcgggg cgctcgagcg cgacggagta
    13261 tggagcggcg agatccgcaa cattcgccga gacggcacgc tgatctggac ggtggcgcac
    13321 atctccacat tcgagcatcg cgaccacggg accgtctgga tctcggtcca gagcgatgtg
    13381 acgccgcgga aggccgcgca ggacgccatg cgtgaggccg aagagcgctt ccgaggagtg
    13441 ttcgagcaca gcccgttcgg gatcgcgctc cttgggcagg acctgcgcct ccgcgagacc
    13501 aaccgtgcct tcggcgagat caccggctac tcacccgatg agctcgtcgg cgagtcgctg
    13561 ccggagttct cagtcgtgtt caacggccat cagtcgaatc accgcgtggt gacgcggctc
    13621 gccacgaaga gcggcgagac ggttccggtc gcgatcaccg gcacagcggt ccacggcgcg
    13681 gacggggatc ccctgtacgg catcgcggtc gtgcagccga tcgacgcctg acgctccggt
    13741 gtccatcccc gcagaacggg taacggcagt cccaacgcaa cccgaccagg ggagatgtga
    13801 tggaaggcag ccaggagacc ggccaggtca caggcgtcaa ggacaaggac tacaacatca
    13861 tctggttcac ggagcagtcg ctgagcaacg cgctgcgcct ggagacctac atccaggacg
    13921 cccagcgtga aggcgactcc gagctcgccg acttcttccg gcgcgctcag gaggcgagcc
    13981 gcaagggcgg tcagcagggc aaggagctgc tgcgcgcccg cctaggctga cctgaacggc
    14041 ccggccgcga gctaggcgtc gcgttgcgcc ggctcgcggc tgtcgagccc aagccggtgc
    14101 gccaccgcgg ccgcctcggt tcggctgcgc acgccgagct tcgagaggat gcgggagacg
    14161 tgcacgctcg cggtcttctc ggccatgaag agcgtgtcgc cgatctcgcg gttggtcctg
    14221 ccgtcggcca cgagcgcgag cacctgtcgt tcgcgcggcg tcaggccgaa gggctcttcc
    14281 tcctcgacct cgccgggctc gggctcttcc gcggcggcct cgtcctcgag ccgcaggcgc
    14341 gcgcgggcga tcaggccttc aacctcgcgc tcgagccatc cggccccgag ctcacgagcg
    14401 gtacgaagtg cattgccggc ccacaacgcc gcctgctcgc ggtcgccggc ctgcacgtgg
    14461 gcctctgccc cgcgccatct cgcgagcgcc gcctcgtagg gccactcgag gcggtcccag
    14521 gcggaggctg cctcggccca gcacgagggc tcggggatgc catcgacccg cgtcccctct
    14581 gcctcggcca tggcgagcca ggcacgctcg accggccggt cgccttccgc gcctgcgcgc
    14641 agacgcgaga ccatcgcctc cgcgcggaat tgcgcgagtg ccgtgtcctc gcccaggtcg
    14701 cgtgcacgct cgatcgcggc cgcctcgatc gcgacgccga tggccgacag gcgcacgatc
    14761 cggttggcgt cctcgctgca gaactcgatc cggtcgagcg cctcgtcgac cgcggcccgc
    14821 gcggcgtcga tgtcgccctc gcgcaacgag agcgatgcgc gtagcgcacc ccaggtgccg
    14881 aggaactgcg gctcggtcga ctcggcgacg aactcctcga tttcctccag gtaccggcgc
    14941 gccgtggcgt gatcgccacg tccgagcgca aacgtcgcgc gctgtagcag cacgtacatc
    15001 gtggtggtcg ccacgtaacg ccgttcggaa ggcggcatcc aggcctccgc ctcatcccac
    15061 tcgccgagct gccacttcag ctcggcgatc atcaccgaca gccagtctcg gggccgggag
    15121 aaccccgaca ccacttcgcg gccacgctcc acgacggcaa gcgcctcgcg cgtgcggcc
//
LOCUS       DASVXS010000030         5454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_149841, whole genome shotgun sequence.
ACCESSION   DASVXS010000030 DASVXS010000000
VERSION     DASVXS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149841"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VF752_01575"
     CDS             <1..979
                     /locus_tag="VF752_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX8854261.1"
                     /translation="AGGALRVGPRSAGAVPGPAAYGRGGTEPTVTDANLLLGYLDATS
                     PLAGGVELDRDAAERAVGGLAGELGLSTIEAAEGIVRVASSEMARAVRVMTVERGTDP
                     RELVLLPFGGAGPLHAAAIAEELDMTRVIVPRASGVLSALGLLVSERRRDLVESVLLS
                     GDELAEERVADVVERLAERGREELSDPTAEVRAAYDLRYRGQAFELTVDGEPAPDPAD
                     LRRAFEAEHERRYGYADPDAQLELVTVRVAVALPGAELAAPRAAAEPTVSEREVTIRG
                     ERLRATIHRGSFERVEGPAICELAEATLVVPPGWSGGEDVDGTISIERR"
     gene            979..2472
                     /locus_tag="VF752_01580"
     CDS             979..2472
                     /locus_tag="VF752_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX8854262.1"
                     /translation="MDPVTLQVMLGALRAACDEMGVVLVRSAHSANIKERRDSSTALF
                     DAEGQMVMQAEHIPVHLGAMPSAVEAVRAEEQRPGDAWILNDPYRGGTHLPDITMVSP
                     IFAAGELTGFAASRAHHADVGGPFPGSMPAGSRTLEEEGVVIPPSRLVLEGRLDQPLL
                     DELVSRMRNPGQRRADLRAQLAANRTGEERMRELADRYGRATLDQAMDETLDYAERRT
                     RARIAEMEDGEREATDVLEAPEGDLELHLRATVRGASMQLDFGGSAAQHDGNLNCPLA
                     VTLSACYFALRVLTDPDVPPCAGAYRPLTVTAPEGSLLNARPPAAVVAGNVETSSRVA
                     DLVLAAFGRALGQGTMNNLTLGNDRFTYYETLGGGQGACPDADGPRAVHVAMSNTLNT
                     PIEALELEFPLRAVEYSLRHGSGGAGRFSGGDGVVRELEALEPMRFSLLTERRRHAPP
                     GAAGGEAGAPGRNLLNGAELPPKASGELQAGDRLRIETPGGGGNGRR"
     gene            2523..3401
                     /locus_tag="VF752_01585"
     CDS             2523..3401
                     /locus_tag="VF752_01585"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8854263.1"
                     /translation="MEAIERVAFIGLGIMGSRMAANLCRAGFEVHAWNRTREKAEQLA
                     EQHGAQVADTPRDAAAASQAVITMVVDSPDVEAVIFGPDGAAAGMHEGDIAIDCSTIA
                     PEASRAIGERLAKQGVAFLDAPVTGSSPKAEDGTLTIMVGGEKEAFDRARPLLDAMGE
                     LIVHVGDQGQGEMAKLINNMVAATNAAALAEALLLAQATGVNSDALVRIMAAGSGNSA
                     MLGLKARPMLERHFDPLFKLDHMLKDVRHALDSARDAKAAMPLGERIEGLYARAAEEG
                     HGDEDFAAVILSIEEE"
     gene            3554..4183
                     /locus_tag="VF752_01590"
     CDS             3554..4183
                     /locus_tag="VF752_01590"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX8854264.1"
                     /translation="MPIALKEWAVTVRALAEGEQLLTLRKGGIREEHKHFEIEHDRFF
                     LYPTFDHQRNDLVRESHKSELGRALEEGVWAAGEPPARALLQDGGVPQPERVRIRAWA
                     EVAASYLITDPRCVDALSPYYVWSTDYAEKRLAWKRRHPLHVIMLRTYRIPRPVTVKV
                     RDEYAGCRSWVELHRELPFEGTPVLSDDEFERAAAEIEAIASEAVPVLA"
     gene            4220..>5454
                     /locus_tag="VF752_01595"
     CDS             4220..>5454
                     /locus_tag="VF752_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8854265.1"
                     /translation="MPAGLRRLVAGTAVALGLLAGVGSPALAAGSKSSQLPIPLPIPV
                     PPGSDSGPAPGGYQQNDGKGFWNILPSGENGLANGPQLAAFLTTGQRPPHSNDQLAMY
                     GDLVYATPGLTAADIPKYFKDASFGVKDGDVERRYSPRSDVTIVRDKGFGVPHIYGST
                     RAGAMFGIGYATAEDRLFFIDVLRHLGRAQLSSFAGGAPANRAFDEEQWQVAPYTEKD
                     LEDQINKFPRYGADGVMLHDDLVKYVAGINQYISEAKLDPTKMPGEYIAINKPLGPDP
                     WKGTDVVATAALVGAIFGKGGGGELNDAAVLQAAQKRFGLKRGLRVWKDFRSANDAER
                     PTTVHKARFPYELTPKKIARGSLAIPDRGSFKELDTASGNTSSTSASASSSGIGGLLR
                     FPGAMSNALVVSGAESQSGR"
BASE COUNT          840 a         1908 c         1964 g          742 t
ORIGIN      
        1 cgccggcggc gcgctccgtg tggggccgcg ctcggcgggc gccgtgccgg gcccggccgc
       61 ctacggccgc ggcggcaccg agccgaccgt gacggacgcg aacctgctgc tcggctacct
      121 cgacgcgacg tcgccgctcg ccggtggcgt ggagctcgac cgcgacgcgg ccgagcgcgc
      181 cgtgggcggg ctggctggcg agctcggcct gagcacgatc gaggccgccg agggcatcgt
      241 gcgcgtcgcg agctccgaga tggcgcgcgc cgtgcgcgtg atgacggtgg agcgcggcac
      301 cgatccccgc gagctcgtgc tgctcccgtt cggcggcgcc gggccgctcc acgcggcggc
      361 gatcgcggag gagctcgaca tgacgagggt gatcgtgccg cgcgcttcgg gcgtgctctc
      421 ggcgctcggc ctgctcgtgt ccgagcggcg ccgcgacctc gtggagagcg tgctgctctc
      481 cggcgacgag ctcgccgagg agcgcgtggc agatgtggtc gaaaggctcg ccgagcgcgg
      541 gcgcgaggag ctgtccgatc ccactgcgga agtgcgcgcc gcgtacgacc tgcgttatcg
      601 cggccaggcg ttcgagctga ctgttgacgg cgagcctgcg ccggacccag ccgacctgcg
      661 ccgagcattc gaggcggagc acgagcgccg ctacggttac gccgacccgg acgctcagct
      721 cgagctggtg accgtgcggg tggcggtcgc gctgcccggc gccgagctcg ccgcaccgcg
      781 ggcagccgcc gaaccgactg tgtcggaacg cgaggtgacg attcggggcg agcggcttcg
      841 cgcgaccatc caccgcgggt cgttcgagcg cgtggagggc cccgctatct gcgagctcgc
      901 ggaggcgacg ctggtcgtgc cgcccggatg gagcggcggc gaggacgtcg acggaacgat
      961 ctcgatcgag aggcgttagt ggacccagtc acgctccagg tgatgctcgg cgcgctccgc
     1021 gcggcctgcg acgagatggg agtggtgctc gttcgatcgg cgcactcggc gaacatcaag
     1081 gagcggcgcg attcctccac cgcactgttc gacgccgagg gccagatggt gatgcaggcc
     1141 gagcacatcc cggtgcacct cggcgcgatg ccgtccgccg tcgaggcggt gcgcgcggag
     1201 gagcagcgcc cgggcgacgc ctggatcctc aacgacccct accgcggcgg cacccatctt
     1261 cccgacatca cgatggtgtc ccccatcttc gccgccggcg agctcaccgg attcgcggcc
     1321 agccgcgcgc atcacgccga cgtcggcggg ccgttccccg gcagcatgcc cgccggctcg
     1381 cgcacgctcg aagaggaggg cgtcgtgatt ccgccgagcc gcctcgtcct cgagggacgc
     1441 ctcgaccagc cgctgctcga cgagctcgtc tcgcggatgc gcaatccggg gcagcggcgg
     1501 gccgacctgc gcgcgcagct ggccgcgaac cgcaccggcg aggagcgaat gcgcgagctg
     1561 gcggaccgct acggccgcgc cacgctcgac caggcaatgg acgagacgct cgactacgcc
     1621 gagcgccgga cgagagcccg catcgccgag atggaggacg gcgagcgcga ggcgaccgac
     1681 gtgctcgagg ccccggaagg cgacctcgag ctgcacctgc gcgcgaccgt gcgcggcgcc
     1741 tcgatgcagc tcgacttcgg cggttcagcc gcgcagcacg acggcaacct caactgcccg
     1801 ctcgccgtga cgctctcggc ctgttacttc gccctccggg tgctgaccga ccccgacgtg
     1861 ccgccctgcg ccggggccta caggcctctc accgtgacgg cgccggaggg cagcctgctg
     1921 aacgcccgcc cgccggccgc ggtggtcgca ggcaatgtgg agacgtcctc gcgcgtcgcc
     1981 gatctggtgc tggcggcgtt cgggcgcgcg ctcggccagg gcacgatgaa caacctcacg
     2041 ctcgggaacg accgcttcac ctactacgag acgctcggcg gcggccaggg cgcctgcccc
     2101 gacgccgacg ggccaagggc cgtgcacgta gcgatgagca acacgctcaa caccccgatc
     2161 gaggcgctcg agctcgagtt ccccctgcgc gccgtggagt actcgctgcg gcacggctcg
     2221 ggcggtgccg gccgcttcag cggcggcgac ggcgtggtcc gcgaactcga ggcgctcgag
     2281 ccgatgcgct tctcgctgct caccgagcgc cgccgtcacg cgccgccggg cgcggccggc
     2341 ggcgaggccg gcgcaccggg gcggaacctc ctgaacggcg cggagctgcc gccgaaggcg
     2401 agcggcgagc tgcaggccgg cgaccggctg cggatcgaga ccccgggcgg cggcggaaac
     2461 gggcggcgct gagccgcgcg ttacgctgcc ccgccgggaa gaacgctcac cgaaggaggg
     2521 caatggaggc gatcgaacgc gtcgcgttca tcgggctcgg gatcatgggc tcgcggatgg
     2581 cggcgaacct ctgccgcgcc gggttcgagg tccacgcctg gaatcgcacg cgcgaaaagg
     2641 cggagcagct cgcggagcaa cacggggccc aagtggccga cacgccgcgt gacgcggccg
     2701 cggcgtcgca ggcggtgatc acgatggtgg tcgactcgcc cgacgtggag gccgtgatct
     2761 tcgggccgga cggcgcggcg gccggaatgc acgagggaga catcgcgatc gactgctcca
     2821 cgatcgcgcc cgaggcgagt cgggcgatcg gcgaacggct cgccaagcag ggcgtcgcct
     2881 tcctcgacgc gcccgtgacc ggctctagcc cgaaggccga agacggcacg ctcacgatca
     2941 tggtcggagg cgagaaggag gctttcgacc gcgcccggcc gttgctcgac gcgatgggcg
     3001 agctgatcgt gcacgtgggc gatcagggcc agggcgagat ggcgaagctg atcaacaaca
     3061 tggtcgccgc caccaatgcg gcggcgctcg ccgaggcgct tctgctggct caggcgaccg
     3121 gcgttaactc cgacgcactc gtgagaatca tggccgccgg atcgggtaac tcggcgatgc
     3181 tcgggttgaa ggcgcgcccc atgctcgagc ggcacttcga tcccctgttc aagctcgacc
     3241 acatgctcaa ggacgtccgg cacgcactgg actcggcgcg cgatgcgaag gccgcgatgc
     3301 cgctcggaga gcggatcgag ggcctctacg cgcgggccgc ggaagaggga cacggggacg
     3361 aggactttgc ggccgtgatc ctctcgatcg aggaggaatg agacgaaaat cacgttgact
     3421 ggcgcgtcag ccaacttgaa ttcggacatc aaatccgtac aatccctggc ggttccgcgc
     3481 cagccccggt gcgtcagccc ggtgcgcgac ggaacctttc tcttctagtt ctttcttctt
     3541 ctgacctcta tctatgccga ttgctctcaa agaatgggcc gtgaccgtgc gcgccctcgc
     3601 cgaaggtgag cagctgctca ccctccgcaa gggcgggatc cgcgaagagc acaagcactt
     3661 cgagatcgag cacgaccggt tcttcctcta tccgaccttc gatcaccagc gcaacgacct
     3721 cgtgcgcgag tcccacaagt ccgagctcgg gcgcgcgctc gaggagggcg tgtgggccgc
     3781 cggcgagccg ccggcgcgcg cgctgcttca ggacggcggc gtgccgcagc cggagcgcgt
     3841 tcgcatccgc gcgtgggccg aggtggccgc gagctacctg atcacggacc cgcgctgcgt
     3901 tgacgcgctc tcgccctact acgtgtggtc caccgactac gcggagaagc gcctggcgtg
     3961 gaagcgccgt catccgcttc acgtgatcat gctccgcacc taccgcatcc cgcgtccagt
     4021 caccgtcaag gtgcgcgacg agtacgcggg ctgccgctcg tgggtggagc ttcatcgcga
     4081 gctcccgttc gagggcacgc ccgtgctctc cgacgacgag ttcgagcggg ccgccgccga
     4141 gatcgaggcg atcgcgtcgg aagcggttcc cgtcctcgcg tagcacaacc ggcgcggcac
     4201 cagcacgctt tgtgattgag tgcccgccgg tttgcgccgg ttggttgcgg ggacagctgt
     4261 cgcgcttgga ctcctggcgg gggtaggttc gcccgcgctc gcggccggat cgaagtcctc
     4321 gcagctcccg atcccgctgc cgataccggt gccgcccggc tccgactccg ggccggcgcc
     4381 cggcggttac cagcagaacg acggcaaggg cttctggaac atcctgccgt cgggcgagaa
     4441 cggactggcc aatgggcccc agctggcggc cttccttacg accgggcaac gcccgccgca
     4501 cagcaacgac cagctcgcga tgtacggcga cctggtctac gccacgccgg ggctcacggc
     4561 ggcggacatc cccaagtact tcaaggacgc gagcttcggc gtgaaggacg gcgacgtcga
     4621 gcgaaggtac agcccgcgca gcgacgtcac gatcgtccgc gacaagggct tcggcgtgcc
     4681 gcacatctac ggctccactc gcgcgggagc aatgttcggg atcggctacg cgaccgccga
     4741 ggaccgcctc ttcttcatcg atgtgctgcg ccacctcggt cgcgcgcagc tctcgagctt
     4801 cgcgggcggg gcgccggcca accgcgcctt cgacgaggag cagtggcagg tggctccgta
     4861 cacggagaag gacctcgagg accagatcaa caagttcccg aggtacggcg ccgacggcgt
     4921 gatgctgcac gacgacctcg tgaagtacgt ggccggcatc aaccagtaca tcagcgaggc
     4981 aaagctcgac ccgacgaaga tgccgggcga gtacatcgcc atcaacaagc cgctcggtcc
     5041 ggatccatgg aagggcaccg acgtcgtcgc gaccgccgcg ctcgtgggcg cgatcttcgg
     5101 aaagggcggc ggcggcgagc tgaacgacgc cgccgtgctc caggccgctc agaagcgctt
     5161 tggcctcaag agggggctgc gcgtgtggaa ggacttccgc tccgcgaacg acgcggaaag
     5221 gccgaccacg gtgcacaagg cgaggttccc gtacgagctc acgccgaaga agatcgcccg
     5281 cggcagcctc gcgatccccg accggggctc gttcaaggag ctcgacacgg cgagcgggaa
     5341 cacgagctcg accagcgcgt ccgcaagcag ctctggcatc ggcggcctgc tccgcttccc
     5401 aggcgcgatg tccaacgcgc ttgtggtgtc gggcgcggag tcgcagtcgg ggcg
//
LOCUS       DASVXS010000031         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1282252, whole genome shotgun sequence.
ACCESSION   DASVXS010000031 DASVXS010000000
VERSION     DASVXS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282252"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..772)
                     /locus_tag="VF752_01600"
     CDS             complement(23..772)
                     /locus_tag="VF752_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX8854266.1"
                     /translation="MVDTHCHLDSIREPTAAVVERAREAGVRRLASVGMHDDSIATTL
                     RIAHEHDEVFAIVGRHPHHSEGFDRADLARIEEAATDERVRAIGETGLDWFRDRAPRD
                     DQRRAFEAQIQLAARLELPLVIHTRDAEEDTFAMLREHAADVTVVMHCFSSPSRLDEC
                     VERRYLCSFAGNVTYPKNVELQAAARDVPEELLLVETDSPYLAPQPVRGKPNEPRNVT
                     MTAEAVARMRGVPYEDLEAAVERNAARVFKW"
     gene            complement(781..2295)
                     /gene="metG"
                     /locus_tag="VF752_01605"
     CDS             complement(781..2295)
                     /gene="metG"
                     /locus_tag="VF752_01605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX8854267.1"
                     /translation="MSFYVTTPIYYVNAQPHLGHAYTTIAADVLARHMRQRGEDVFFL
                     TGTDEHGEPVALAAEKLGVSPRELADQNSVRFRDLVAAMNASNDFFIRTSDPEHEAKV
                     QEVVQRIHGNGYVYPGHYEGWYCPRCADFKTDAELIDGNKCPIHLIELEREVEDNWFF
                     KLSAFQEPLEKLYAEHPDFVLPDVRFNEALSFIKMGLNDISLSRARISWGVPVPWDES
                     QVIYVWIDALLNYYTALSYAREGEDLTERFWPADVHVIGKDILKFHAVIWPAMLMAAG
                     IEVPKRVFIHGYLLMDEHKMSKSLGNVIDPFEVIDMYGVDALRFYVLREVAFGQDGNV
                     STGGFESRYTTELANGYGNLASRTLAMIARYRDGVVPDAEPDPALDPDIAGVAERVCE
                     HFDRQDVSGALEEIWQVVRRLNRYVQDEQPWQLAKDDAQTQRLDQVLYSLAEGLRVVS
                     VLVHPVIPESAEKLLEALGRDDLSLGNARFGAVPGGAETSELAPLFPRVERAPA"
     gene            complement(2356..3123)
                     /locus_tag="VF752_01610"
     CDS             complement(2356..3123)
                     /locus_tag="VF752_01610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854268.1"
                     /translation="MATDGTPATFYDDLASDYHLIFADWNASIHRQAGVISALLREHG
                     LTRGGVLDASCGKALGLAEAGYSVTATDISPVSVERCAREAAARGLTVTTGVADLRTL
                     DRDVAGPFDAVVSFDNALPHLLDDADLLAAAESVRRVLRPGGVLLASIRDYDTLLKER
                     PSGDLPRRFQSDSGERVVVQLWDWFETDRYTARHFIIDGSPAGCWTVAERTTSYRALS
                     RATLSDVLQHAGLEAICWQMPERTGFYQPVVTARAPA"
     gene            complement(3226..>3489)
                     /locus_tag="VF752_01615"
     CDS             complement(3226..>3489)
                     /locus_tag="VF752_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854269.1"
                     /translation="GVGRPSVPRASAGPAAALEPRSLRHARAATTQPANSHRGIDYGW
                     LAACAGLIAVASVLGRPERAKTAARASRSAVRRIRHTAARASG"
BASE COUNT          533 a         1179 c         1217 g          560 t
ORIGIN      
        1 ctggcctggc gggtctctta ggtcaccact tgaagacacg cgcggcgttg cgctccaccg
       61 ccgcctcgag gtcctcgtac ggcacgcccc tcatgcgcgc gaccgcctcc gcggtcatcg
      121 tcacgttgcg cggctcgttc ggcttgccgc gcacgggctg aggggcgagg tacggcgagt
      181 ccgtctcgac cagcagaagc tcctccggca cgtcacgagc cgccgcctgc aactccacgt
      241 tcttcgggta ggtgacgttg ccggcgaacg agcacaggta ccgccgctcg acgcattcgt
      301 cgagccggct cggcgaggag aagcaatgca tcaccaccgt cacgtcggca gcgtgctcgc
      361 ggagcatcgc gaaggtgtcc tcctcggcgt cgcgcgtgtg tatgacgagc ggcagctcga
      421 gccgagcggc gagctggatc tgcgcctcga aggcgcgcct ctggtcatcg cgcggggcgc
      481 ggtcgcggaa ccagtcgagg ccggtctccc cgatcgcgcg tacgcgctcg tccgtcgctg
      541 cctcctcgat tcgcgcgaga tcggctcggt cgaagccctc cgagtggtgc ggatggcgcc
      601 cgacgatcgc gaatacctcg tcgtgctcgt gcgcgatccg gagcgtggtc gcgatcgagt
      661 catcgtgcat cccaacgctc gccagcctgc ggacgccggc ctccctggcg cgctccacca
      721 ccgcggcggt gggctcccgg atggagtcga ggtggcagtg ggtgtcgacc accgcgcctc
      781 ctacgcgggg gcgcgctcga ccctcgggaa cagcggcgcg agctcgctcg tctcagcgcc
      841 gcccggcact gccccaaagc gcgcgttgcc gagcgacaag tcgtcgcgcc cgagcgcctc
      901 gagcagcttc tccgccgact cggggatcac cgggtgcacg agcaccgaca cgacgcgcag
      961 cccctcggca agcgagtaga gcacttggtc gagccgctgg gtttgggcgt cgtccttggc
     1021 cagctgccac ggctgctcgt cctggacgta gcggttcaga cgccgcacta cctgccagat
     1081 ctcctcgagc gccccgctca cgtcctgccg atcgaagtgc tcgcagacgc gttcggcgac
     1141 gccggcaatg tcggggtcca gcgcgggatc gggctcggca tcgggtacga cgccgtcgcg
     1201 gtagcgcgcg atcatcgcga gcgtgcggct cgcgaggttg ccgtacccgt tggcgagctc
     1261 ggtcgtatag cggctctcga agccgccggt cgacacgttg ccgtcctggc cgaaggcgac
     1321 ctcgcgcagc acgtagaagc ggagggcgtc gacgccgtac atgtcgatga cctcgaacgg
     1381 gtcgatcacg ttgccaagcg acttcgacat cttgtgctcg tccatcagca ggtagccgtg
     1441 gatgaagacg cgcttcggca cctcaatgcc ggcggccatc agcatcgccg gccagatgac
     1501 ggcgtggaac ttgaggatgt ccttgccgat cacgtgcacg tcggccggcc agaagcgctc
     1561 ggtgaggtcc tcgccctcgc gcgcgtatga gagcgccgtg tagtagttga gcagcgcgtc
     1621 gatccagacg tagatcacct gactctcgtc ccacgggact ggcacgcccc agctgatgcg
     1681 cgcgcggctc agcgagatgt cgttgaggcc catcttgatg aacgagagcg cctcgttgaa
     1741 gcggacgtcc gggaggacga agtccgggtg ctcggcgtac agcttctcca gcggctcctg
     1801 gaacgcggac agcttgaaga accagttgtc ctcgacctcg cgctcgagct cgatcaggtg
     1861 gatcgggcac ttgttgccgt cgatcagctc cgcgtccgtc ttgaagtcgg cgcagcgcgg
     1921 gcagtaccag ccctcgtagt ggccggggta cacgtagccg ttgccatgga tccgctgcac
     1981 gacctcctgc accttcgcct catgctcggg atccgaggtg cggatgaaga aatcgttgct
     2041 cgcgttcatg gcggccacga gatcgcggaa gcgcaccgag ttctggtcgg cgagctcgcg
     2101 cggcgagacg cccagcttct cggcagcgag cgccaccggc tcgccgtgct cgtcggtgcc
     2161 ggtgaggaag aacacgtcct cgccccgctg gcgcatgtgc cgcgcgagca cgtcggcagc
     2221 gatcgtggtg taggcgtggc ccagatgcgg ctgggcgttc acgtagtaga tgggcgtggt
     2281 gacgtagaag gacatggaga gtcgatgcta cttgcggcgg aacgtccgac ttggcgtcga
     2341 attgtgaagt ggtcctcagg ctggcgctcg tgcggtgacg accggctggt agaagcccgt
     2401 ccgctccggc atctgccagc agatggcctc gagccccgcg tgctgcaaca cgtcggagag
     2461 cgtcgcgcgg gacagcgcgc ggtagctggt cgtccgttcg gcgacggtcc agcatccggc
     2521 tgggctgccg tcgatgatga agtggcgcgc tgtgtagcgg tccgtctcaa accagtccca
     2581 gagctggacg acgacgcgct cgccggagtc gctctggaat cggcggggta ggtcaccgga
     2641 cgggcgttcc ttcaggagcg tgtcgtagtc gcgaatggag gccagcagca cgccgccggg
     2701 gcgcagcact cggcgaaccg actcagcggc ggcaagcagg tcggcatcgt cgagcaggtg
     2761 cggaagcgcg ttgtcgaacg acaccaccgc atcgaacgga cccgctacgt cgcggtccaa
     2821 tgtccgcagg tcggccacgc cggtggtgac cgtcaacccg cgcgcagccg cctcgcgggc
     2881 gcaccgctcg acggaaaccg ggctgatgtc ggtcgcggtc acggagtacc ccgcctccgc
     2941 gagaccaagg gccttgccgc acgacgcgtc cagcacgccg ccgcgggtca gtccgtgctc
     3001 gcgcagcagt gcgctgatga cgccggcctg tcgatgaatc gacgcattcc agtcggcgaa
     3061 gatgaggtgg taatcgctgg ccagatcgtc gtagaacgtc gctggagtgc cgtcggtggc
     3121 catgcgttga cgataagggg cagcacgtac ttcgcttgcc ggcagcctca gcgcggttcc
     3181 ctcccgcgca tcgccccacg tcgaagcgac cggacgggaa tcctcttagc ccgaggcccg
     3241 cgcggccgta tgccggattc ggcgcacggc tgagcggctt gcccgcgcag cggtcttggc
     3301 ccgctcgggc cgtccgagga cgctcgcgac ggcgatcagt ccggcgcaag cggcgagcca
     3361 gccatagtca attccgcgat gcgagttggc cggctgcgtg gtcgcggcgc gtgcgtgccg
     3421 caaagaccgc ggctcgagcg cggcggccgg ccccgccgag gcacgcggca cagacgggcg
     3481 accgactcc
//
LOCUS       DASVXS010000032        12265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_570526, whole genome shotgun sequence.
ACCESSION   DASVXS010000032 DASVXS010000000
VERSION     DASVXS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12265
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570526"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VF752_01620"
     CDS             <1..432
                     /locus_tag="VF752_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854270.1"
                     /translation="TLTQGHLAQHVLFHYFHQPGLGIRARSIFGVSARRYQRLRLSGR
                     SPAEIAAAHGVSRARLAKRAMKRFRAIQLRGVALHATTRRQAARFLRRQRAGLSHWLD
                     SHIRKPGFGKTRRHRRTHSLPRRALLCFLLRGHSDPRGESR"
     gene            complement(439..1467)
                     /gene="trxB"
                     /locus_tag="VF752_01625"
     CDS             complement(439..1467)
                     /gene="trxB"
                     /locus_tag="VF752_01625"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX8854271.1"
                     /translation="MSEARTENVIIIGSGPAGYTAALYTSRADLEPFVAEGFLWGGLL
                     QQTTDVENYPGFPDGIMGPEMMQKFREQAERFGTRFVTEDVTKVELSQNGGVHRVWIG
                     DEEHRARAVILAMGAEPKKLGVPGEEELAARGVSYCATCDAAFFRGKQTIVVGGGDTA
                     MEDAIFLSKFAEKVTIVHRRDDFRASQIMLDRARAAENIELLTPYVVERFEDSGVGQL
                     GHAILRNAETGEEKTLELDGAFVAIGHRPNSHLVDGQIELDENGYVLTDGNSTRTKVP
                     GVFAAGDLVDHTYRQAVTAAGSGCQAALDAEWFLRDTPPDAEREWEPDPDKVAAAAER
                     AAAQAAQS"
     gene            complement(1464..2660)
                     /locus_tag="VF752_01630"
     CDS             complement(1464..2660)
                     /locus_tag="VF752_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX8854272.1"
                     /translation="MSEELRKDTAQPEVHAPETEEIEQAGTRLLEDKRRIVGLLLAFV
                     LLIVAIYVLLPKVAGVGGALHRLGNPKWGWIVVALAFTVCAFLAYGACFRELMTTRAR
                     RQIVQRLQFGPTYEITVAAFGATALFSAGGAGGIALTYWALRRAGMPRRETVGREVAF
                     LAVLYTIYLGTVLVFGVLLRTGVLNGAAPVAGTIVPAGLAGGALLIIGLLALIPGDME
                     RRIRQLGSGGRFSRVAERVAAAPATVSTGVRFALSLLSRPRYASMAILGAFGYWAAML
                     GILWASFKAFGGGVPLGVLVQGFFVGMAANLAPSPAAGVGTLDAGLIGSFLLFDQPSH
                     TVFPAVLAFRLVGFWLPIIPGVIAYFRVRRRVAEWQREDAADRYTIKSKVSQEARTAR
                     LTAEES"
     gene            complement(2660..3541)
                     /locus_tag="VF752_01635"
     CDS             complement(2660..3541)
                     /locus_tag="VF752_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8854273.1"
                     /translation="MGAVAVGIAAPIVRRRLRLRPPVVSVLAWQAPVAMALAVRRSPA
                     RDAAIYALQMWAYIAHYEMPHDDPEALLARLRVDYPIRGDTLIGVGVPPTLRLQRSLG
                     RPGQVTRLDLALSWIHWSWFFVPHGTVAYVLLRHRDQFPRAASLTAAVFDLGLVFYWT
                     LPTAPPWWAGANGNMEHVRRIMVETGEHFWGRAWRPLYDLLGGNPFAAMPSLHFATSV
                     MAAHVLSDVGPVQGAIGWTYAVTLGFALVYLGEHYVVDLAAGLGLAEAVRRLSPAAQP
                     LLLRAARTIQRFEPGMP"
     gene            3738..4124
                     /locus_tag="VF752_01640"
     CDS             3738..4124
                     /locus_tag="VF752_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8854274.1"
                     /translation="MEFQLAGGPYAGTAARLALGNLDGRLDPVMAQDLRLLVTEAVKN
                     SVEHAGVGEDDSIGLKLSLDNARVRVEVSDDGPGFEPDASHPGEETSGWGLFLIDQMS
                     DRWGVVRDNGLTRVWFELDRPEQASA"
     gene            4363..5193
                     /locus_tag="VF752_01645"
     CDS             4363..5193
                     /locus_tag="VF752_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8854275.1"
                     /translation="MKQGYAAAELPTASRPGDCSDSTAAYMRWAQALCRLWLPVIAAV
                     YAYGHHAYASHTSATPIWDFGPQPGGVVHVTVVGRDARSRPGVRVHAVGWLDKRDVLI
                     RHNIPVTSPARTIVDLAETLPPRRLRRTIDEAITSRLATEAQIREALERAPTKKGKRN
                     LSAILDAAVEPALTRSEAEEVFLGIVEAGGLPRPKANAVVHGYEVDFYWPERRLVVEI
                     DGYRYHGHRSAFEHDHARRADLASKGIDVMPFTWRQISETREIVLAQTAAALATRSAP
                     "
     gene            complement(5174..5824)
                     /locus_tag="VF752_01650"
     CDS             complement(5174..5824)
                     /locus_tag="VF752_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8854276.1"
                     /translation="MLIDALDWLGHSGFRLRVGRANVYIDPYRVAAGAPKADLILITH
                     GHYDHFSPQDVELLSHAGTWLVGPAAVAERVSGQVISLRPGETVEDELVRGVHVTAVA
                     AYNTSKRDQSGNVFHPPDAGFLGYDLNVAGERLYHAGDTDVIPEMDEVAGVDVALLPV
                     SGRYVMTPGEAAEAARRIQPRLAVPMHWGSNVGTREDAEEFARKAPVEVRIMEQSE"
     gene            5876..6676
                     /locus_tag="VF752_01655"
     CDS             5876..6676
                     /locus_tag="VF752_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8854277.1"
                     /translation="MAGSYETVIWEVQDGVGRLTLNRPDTLNAWTAEFGAELKQVVED
                     DAAAPAVRAVLVTGAGRGFSSGADLKAGFDPHPDDGMPDVRKELHDVYHPVIAGIRRL
                     EKPVVAAVNGPAVGIGCSLALACDLVLAAESAFFGLAFVNIGLMPDGGSTLFVPAAVG
                     KTRAFEMALLGERVPAPKALEWGLVNFVHADDRLLEEAEGLAARLAAGPTRSYASSKK
                     ALNNMIYPNLDEQLALEAELQHELARTGDFTEGVSAFVDKRQPAFTGA"
     gene            6760..7644
                     /gene="coxB"
                     /locus_tag="VF752_01660"
     CDS             6760..7644
                     /gene="coxB"
                     /locus_tag="VF752_01660"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8854278.1"
                     /translation="MRPDASLLRRRALVLALVGAALVLLLAPAGALADFISPESGGSP
                     NADKIDTLYKIVFFIGVAIFLLVEGLLITSIIRFRARRRGPEPVQVHGNTPLEIGWTI
                     GAAVLVLVIAGITFAFLGPIRHAPNTTVSNLSASEGVQFASAPQVSNPTSKKALHINV
                     TGMQYIWRFDYIDAKRLTENRPVYSYFRMVVPTDTTVIAKINAADVIHSWWIPKLGGK
                     ADGVPGYNNYTWFKISKPGLYKGQCAELCGEGHADMRAQVLALPPDQYRAWIETQRNE
                     LLASQKALAAQRKAGIGQ"
     gene            7666..9381
                     /gene="ctaD"
                     /locus_tag="VF752_01665"
     CDS             7666..9381
                     /gene="ctaD"
                     /locus_tag="VF752_01665"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX8854279.1"
                     /translation="MSTSEYVGQRRPVTALPRPEIVLHGVRDRPRGWLEWLTTTDHKK
                     IGILYIYATFIFFLIGGVEALMMRLQLAVPENTLLAPKAYNAVMTMHGTTMVFLFVVP
                     VLAGFGNYLVPLMIGARDMAFPRLNALSFWLLLFGGIVFYVSIFFHPPEAGWTMYTPL
                     SEEAFSATGGVDAWIFLIHLTGLSSLLGAINFVATIHNMRARGMGWARMPLFIWTILI
                     YSYLLILALPAVAAAVTMLLADRHLDTGFFNPANGGDPMLWQHLFWFFGHPEVYIMIL
                     PSFGIISEVVPVFARKPIFGYKAIAASTAAIAFLGMLVWAHHMFATPMPAVVLSFFML
                     SSFAIGVPTGIKIFNWLATMWRGSIVFKTPMYFACGFIAQFVIGGISGVILAVFPVDW
                     QLHDTYFVVAHFHYVLFGGSIFGLFAGLYYWFPKITGRMMSEGLGKASFWLMFIGFNT
                     TFLIQHSLGMSGMPRRIYTYYPNGDWAAWNLVSTIGSFILGIGVLLTVLNVLLSLKNG
                     KRAGNDPWKANTLEWFVPSPPPENNFDVVPRIRSAEPMKDIRRDVAAAGTPAGSVYQP
                     VAPGT"
     gene            9383..10291
                     /locus_tag="VF752_01670"
     CDS             9383..10291
                     /locus_tag="VF752_01670"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX8854280.1"
                     /translation="MEAGARTRVAVAPPRVGSLRQVVSDYVDMTKPRVQSLLLFTTVT
                     TMYVAGTPSLSRVVLTCLGGALSAGGAGAINHYLDRDIDAQMKRTANRPVPSGRVAPG
                     AALAWGIFLGSASFVLMALTINLLAASLALAGLLGYVVVYTIWLKRTTPQNIVIGGAA
                     GAMPPLVAWAATTGHLAGAALYLFAIVFYWTPPHFWALSLLMKDEYASAGVPMLPVVR
                     GETETRWQILLYTLLLVGVTLLPVAFGVFDGIYLIAALLLGSAFTAMAIRLRRSAERR
                     DALRLYLFSLAYLALLFAAMVADARL"
     gene            10297..11322
                     /locus_tag="VF752_01675"
     CDS             10297..11322
                     /locus_tag="VF752_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8854281.1"
                     /translation="MPGTLAERLTSQLLAGPPATSAVDVAERLLAIQAQDPRGARLAV
                     RARSEGVGASDVDRALTDERTLLITTLNRGTLHMVRSEDYPWLQAVTTPPLVQWNARR
                     LEQEGLSPADSERAVTLLERWLGEDGPLTRPQLRERLTAAGIRIQGQALPHVVMAASL
                     RGLIVRGPMVGKQHAFALVRNWLGEQRPVDRDTARAELARRYLAGHGPASDRDLARWS
                     GLPLRDVRAGLEASARELVQRDDGLLDLAGKPTAADVPEPRLLGSFEPVLLGWTSRDD
                     ILGENEGAIVRGGLFRPFAMVRGRGVATWRLNGNSVELEPFEPISRADMAALRRDAGG
                     VERFLST"
     gene            complement(11312..11806)
                     /locus_tag="VF752_01680"
     CDS             complement(11312..11806)
                     /locus_tag="VF752_01680"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX8854282.1"
                     /translation="MKSGSGRVKYFAYGSNMAPERLAAYSPDCQYVGPARLTDHRLAF
                     NRRSVRTKTGVADISFAYGMTVWGALYDIDEADLATIDEKEGTTIDAYKRMRVTVLTL
                     AEEPVEAITYKVVKEEPAEVTPSSEYVDGLIAGARACGLPQPYLAFLDAIRADCEGSA
                     DVRY"
     gene            11951..>12265
                     /locus_tag="VF752_01685"
     CDS             11951..>12265
                     /locus_tag="VF752_01685"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8854283.1"
                     /translation="MPTTEELASCRGQQVVGPSGDKIGKLEEVYVDSATGKPEWFAVS
                     TGLFGTRLSLIPIAGANLRDDTVQVAYDKAKVKDAPNVDPDGDLSQDQEARLYGHYGL
                     AYS"
BASE COUNT         1904 a         4352 c         4082 g         1927 t
ORIGIN      
        1 acgctcacgc agggccacct cgcgcagcac gtgctgttcc actacttcca ccagccgggg
       61 ctcggcatcc gcgcacgcag catcttcggc gtgtcggcgc ggcggtacca gcgtctgcgc
      121 ctgtccggac gctcacccgc agagattgcg gccgcgcatg gcgtgtcgag ggcccggctg
      181 gcgaagcgag cgatgaagcg cttccgggcg atccagctgc ggggcgtcgc actgcacgcg
      241 accacgcggc gccaggccgc gcgcttcctt cgccggcagc gcgccggcct gtcgcattgg
      301 ctcgactcgc acatccgcaa gccgggcttc gggaagacgc gccgtcatcg ccgcacgcac
      361 tcgctgccgc gacgcgcgct cctttgcttc ctgctgcgcg ggcactccga tccgcgcggc
      421 gagagccggt agtccgggct aactctgcgc tgcctgcgcc gcggcgcgct cggcggcagc
      481 ggcgaccttg tccgggtcgg gctcccactc gcgctcagcg tccggcggcg tgtcgcgcag
      541 gaaccactct gcgtcgagtg ccgcctggca gcccgagcct gcggcggtga ccgcctggcg
      601 gtaggtgtgg tccaccaggt cgcccgcggc gaaaacgccg ggcaccttcg tgcgcgtgga
      661 attgccatcg gtgagcacgt agccgttctc gtcgagctcg atctgcccgt ccacgaggtg
      721 cgaattcggc cggtggccga tcgccacgaa cgcgccgtcg agctcgagcg tcttctcctc
      781 gccggtctcg gcgttgcgca agatggcgtg accgagctgg cccacgccgc tgtcctcgaa
      841 gcgctcgacc acgtacgggg tcagaagctc gatgttctcg gccgcgcgag cgcggtcgag
      901 catgatctgc gacgcgcgga agtcgtcgcg ccggtggacg atcgtcacct tctcggcgaa
      961 cttggagagg aagatcgcgt cctccatcgc cgtgtcgccg ccacccacca cgatcgtctg
     1021 cttgccgcgg aagaaggcgg cgtcgcaggt ggcgcagtac gagacgccgc gcgcggcgag
     1081 ctcctcctcg ccgggcacgc cgagcttctt cggctcggcc cccatcgcga ggatgaccgc
     1141 gcgggcgcga tgctcctcgt cgccgatcca gacgcggtgc acgccgccgt tctgcgacag
     1201 ctcgaccttc gtcacgtcct cggtcacgaa gcgagtgccg aagcgctccg cctgctcgcg
     1261 gaacttctgc atcatctccg ggcccatgat cccgtcgggg aagcccgggt agttctccac
     1321 gtcggtcgtt tgctggagca ggccgcccca gaggaagccc tcggccacga acggctcgag
     1381 atcggcgcgc gacgtgtaca gcgcggcggt gtagcccgcc gggccactcc cgatgatgat
     1441 cacgttctca gtgcgcgctt cgctcacgac tcctccgctg tcagccgggc cgttctggcc
     1501 tcttggctta ctttactttt tatagtgtag cgatcggcgg cgtcttcccg ctgccattcg
     1561 gctacccgcc ggcggacgcg gaaatacgcg atcacgccag ggattatcgg gagccagaac
     1621 cccacgagcc ggaacgccag cactgcgggg aagaccgtgt gactgggctg gtcgaagagt
     1681 aggaacgagc cgatcaagcc ggcgtcgagg gtaccgaccc ccgcggcggg cgacggcgcg
     1741 aggttcgccg ccattccgac gaagaagccc tggaccaaca cgcccagcgg gacacctccg
     1801 ccaaaggcct tgaagctcgc ccagaggatg ccgagcatgg ccgcccaata gccaaaagcg
     1861 ccgaggatcg ccatgctcgc gtagcgcggg cggctcagca gtgagagcgc gaagcgcacg
     1921 ccggtcgaga cggtcgcggg cgccgccgca acccgctcgg ccacgcgtga aaagcgtcct
     1981 ccgctcccca gctgtctgat gcggcgctcc atgtcgcccg ggatcagcgc gaggagtccg
     2041 atgatcaaga gcgcgccccc ggccaagccc gcaggaacga tggtgccagc gacgggcgca
     2101 gcaccgttca gcacaccggt ccgcagcagt acgccgaaca cgagcaccgt gccgaggtag
     2161 atcgtgtaca gcaccgcgag aaacgcgacc tctcttccga ccgtctcgcg ccgtggcatg
     2221 ccggccctgc gcagcgccca gtaggtcagc gcgattccac cggcgccgcc agcggagaag
     2281 agcgccgtgg ccccgaaggc cgcgacggtg atctcgtacg tcgggccaaa ctggagccgc
     2341 tggacgatct gccggcgggc gcgcgttgtc atcagctctc ggaagcaggc cccgtacgcg
     2401 aggaacgcgc agacggtgaa ggccagcgcg accacgatcc agccccactt cgggttgccc
     2461 agacggtgga gcgcgccacc gacgcccgcc accttcggaa gcagcacgta gatggcgacg
     2521 atgagaagga cgaaggcgag caggaggccg acgatccgcc gcttgtcctc gagcagccgc
     2581 gttccggcct gctcgatctc ctccgtctcg ggagcgtgca cctcgggctg cgccgtgtcc
     2641 ttccgcagct cctcggacat cagggcatcc cgggctcgaa tcgctggatc gtgcgcgcgg
     2701 cgcgcagcag cagcggctgt gcggcgggag acagccggcg taccgcctcg gccagaccga
     2761 gtccggccgc caggtccacg acgtaatgct caccgaggta cacgagcgcg aatccgagcg
     2821 tcaccgcgta agtccagccg atcgcgccct ggactggtcc gacgtcgctg agcacatgtg
     2881 ccgccatcac agaggtagcg aagtggagcg aaggcatcgc cgcgaacgga ttgcctccaa
     2941 ggagatcgta gagggggcgc cacgcccggc cccagaaatg ctctcccgtc tcgaccatga
     3001 tccttcgcac gtgctccatg ttcccgttcg cacccgccca ccacggcgga gcggtcggaa
     3061 gcgtccagta gaacacgaga cccaggtcga agacagccgc tgtgagggag gcggcacgcg
     3121 ggaactggtc acgatggcgc aaaagcacat atgcgaccgt cccgtgtggt acgaagaacc
     3181 agctccaatg gatccacgag agcgccaggt cgagccgcgt gacctggccg ggccgcccca
     3241 gggagcgctg aaggcggagc gtcggcggca cgccgacgcc tatcagggtg tcgccgcgaa
     3301 tcgggtagtc caccctcagg cgcgcgagca gcgcctcggg gtcgtcgtgg ggcatctcgt
     3361 agtgggcgat ataggcccac atctggagcg cgtagatggc ggcgtcgcgc gcgggcgacc
     3421 ggcgcacggc gagcgccatg gcgactggcg cctgccacgc gagcacgctg acgacgggcg
     3481 gccgcaggcg caggcggcgt cggacgattg gcgcggcgat acccacggcc acggcgccca
     3541 tggcggcgat ccgtagggcg cggcgcgact gcaaagcgcg cgcagtatag gcggggcagc
     3601 ggccaattcc cccgcccgta gtagtaactt tcggcactcg caaaagcgcc ttcggccgct
     3661 cacggaaggg gaggagaggg agtctcatgt ccgcgcaggg ggacgttcgg aggagcgcag
     3721 acggttccgc aagcgacgtg gagttccaac tagcaggcgg tccatatgcc ggcactgccg
     3781 cgcgcctcgc cctcggaaac ctcgacgggc ggctagatcc tgtgatggca caggacctgc
     3841 gcctgctggt gacggaggcc gtgaagaaca gcgtggagca cgccggagtg ggcgaggacg
     3901 actcgatcgg cctgaagctc tccttggata acgcgcgcgt gcgcgtagag gtgtccgacg
     3961 acggaccggg cttcgagccc gacgcgagcc acccgggcga ggagacctcc ggctggggcc
     4021 tgttcttgat cgaccagatg tcggaccgct ggggcgtggt acgcgacaac gggctgacgc
     4081 gcgtgtggtt cgagctggac cggcccgagc aggcgtccgc gtagcggcgc gcccctcggc
     4141 gcgtctacag cagcaacaag gaggggtccg ggcgggggcg aagccgcgtt cggtcatgca
     4201 tttctgcagg tgactggggg ctagcttgag gtcgcctgca cttagtcatg catgtcatgc
     4261 cttcgtgcag gcggttgacg gctgtggcgc ggctgtctgc agatatgcag gttgcgcgcc
     4321 gagcgcaggg gcagcacgga gtggtgaagc acgcccatct cgttgaagca gggctacgcc
     4381 gcggcggaat tgcccaccgc gtcgagaccg ggcgactgca gcgactccac cgcggcgtat
     4441 atgcgctggg cgcaggccct ctgccgcctc tggctccccg ttattgctgc tgtttacgcc
     4501 tacggccacc acgcgtatgc gagccacacc agcgcgacgc caatctggga cttcggccct
     4561 cagccaggcg gcgttgtgca cgtgacggtg gtgggcaggg acgcgcggag ccgccccggc
     4621 gtccgggtgc acgcggtcgg atggctggac aagcgcgacg ttctaatccg ccacaacatc
     4681 cctgtgacct caccagctcg cacgatcgtg gacctggcgg aaaccctccc ccctcgccgc
     4741 ctccgccgaa cgatcgatga ggcaataaca agccggctcg ccaccgaagc gcagatcaga
     4801 gaggcgctcg agcgagcgcc gaccaagaaa ggcaagcgca acctcagcgc gatcctcgat
     4861 gctgccgttg agcccgccct gacccgcagc gaggccgaag aggtattcct tggcatcgtg
     4921 gaagccggcg gcctcccccg ccccaaggcg aacgcggtcg tccacggcta cgaggtcgac
     4981 ttctattggc ccgagcgccg gctcgtcgtc gagatcgacg gttaccgcta ccacggtcac
     5041 cgctccgcgt tcgagcacga ccatgcgcgc cgggccgacc tcgcatccaa gggcatcgac
     5101 gtgatgccct tcacctggcg ccagatcagc gaaacgcgcg agatcgtgct cgcccaaacc
     5161 gccgcggcgc tcgctactcg ctctgctcca tgatccgcac ctcgaccggt gccttccgcg
     5221 cgaactcctc cgcatcctcg cgcgtgccca cgttcgagcc ccagtgcatc ggcacggcca
     5281 gccgcggctg gattcgccgc gccgcctcgg ccgcctcgcc gggcgtcatc acgtagcggc
     5341 cgctgacggg aagcagcgcc acatccacgc cggcgacctc gtccatctca gggatcacgt
     5401 cggtgtcccc ggcgtggtag agccgctccc cggccacgtt caggtcgtag ccgaggaagc
     5461 ccgcgtcggg cgggtggaac acgttgcccg actggtcgcg cttcgacgtg ttgtaggccg
     5521 cgaccgcggt cacgtgcacg ccgcggacca gctcgtcctc caccgtctcc cccggccgta
     5581 gcgagatcac ctggcccgac acgcgctccg ccaccgcggc cggcccgacc agccacgtgc
     5641 ctgcgtgcga gagcagctcc acgtcctgcg gcgagaagtg atcgtagtgc ccgtgcgtga
     5701 tgaggatgag gtccgccttg ggtgccccgg cagccacgcg gtacgggtcg atgtacacgt
     5761 tcgcgcgccc gacacgtagg cgaaaaccgg aatgcccaag ccagtcgagg gcgtcgatca
     5821 gcaccgatcg aggatagttc cgcacgatcc gcccacgacc ttggaggagc agacgatggc
     5881 cggcagctac gagaccgtga tctgggaggt gcaggacggc gtcgggcggc tcacgctcaa
     5941 ccgccccgac acgctcaacg cttggaccgc cgagttcggc gcggagctga agcaggtcgt
     6001 tgaggacgac gcggccgccc cggcggtgcg ggccgtgctc gtgaccggag cgggccgcgg
     6061 cttctcgtcg ggcgccgacc tcaaggcggg cttcgacccc catcccgacg acggcatgcc
     6121 cgacgtccgc aaggagctgc acgacgtcta ccacccggtg atcgccggga tccgccgcct
     6181 cgagaagccc gtggtggccg ccgtgaacgg gccggcggtc ggcatcggct gctcgctcgc
     6241 gctcgcgtgc gacctcgtgc tcgcggcgga gtcagccttc ttcgggctcg ccttcgtgaa
     6301 catcggactg atgccggacg gcggctccac gctgttcgtg cccgcggcgg tcggcaagac
     6361 gcgcgccttc gagatggcgc tgctcggcga gcgcgtgccc gctccgaagg cgctcgagtg
     6421 gggcctcgtg aacttcgtcc acgccgacga ccggctgctc gaggaggccg aggggctggc
     6481 ggcgcgtctc gcggccggcc cgacccgttc ctatgcctcc tcgaagaagg cgctgaacaa
     6541 catgatctat ccgaacctcg acgagcagct ggcgctcgaa gccgagctcc agcacgagct
     6601 cgcgcgcacg ggagacttca ccgagggcgt gagcgcgttc gtggataagc ggcagccagc
     6661 gttcaccggg gcttgagcgc catcccgctt ggggagcgga ttttggggca tttgtcacga
     6721 ccatctgacg acggccgcgc aggtagtatt ccgcgcgcct tgcgacccga cgcgtctctt
     6781 ctgcgccgcc gcgccctggt tcttgccctg gtcggggccg ccctcgtgct gctcctggcg
     6841 cccgcgggcg cgctcgccga cttcatctcc ccggagtccg gcggctcgcc gaacgccgac
     6901 aagatcgaca cgctctacaa gatcgtcttc ttcatcggcg tggcgatttt cctgctggtc
     6961 gagggtctcc tcatcacctc gatcatccgc ttccgcgccc gccgccgcgg gccggagccc
     7021 gtccaggtcc acggcaacac gccgctcgag atcgggtgga cgatcggtgc tgcggtgctg
     7081 gttctggtca tcgcgggcat cacgttcgca ttcctcggcc ccatccgtca cgcgccgaac
     7141 acgaccgtga gcaacctcag cgcctcggaa ggcgtccagt tcgcgagcgc tccgcaggtg
     7201 tcgaacccga ccagcaagaa ggcgctccac atcaacgtga ccggcatgca gtacatctgg
     7261 cgcttcgact acatcgacgc gaagcgcctc accgagaacc ggccggtcta ctcgtacttc
     7321 cgcatggtcg tgcccaccga cacgaccgtg atcgcgaaga tcaacgcggc cgatgtgatc
     7381 cactcctggt ggatccccaa gctcggcggc aaggcggacg gcgtcccggg ctacaacaac
     7441 tacacctggt tcaagatctc gaagcccggc ctctacaagg gccagtgcgc agagctctgc
     7501 ggcgagggcc acgcggacat gcgcgcccag gtgctggcgc tgccgcctga ccagtaccgc
     7561 gcctggatcg agacgcagcg caacgagctg ctcgcctcgc agaaggctct ggcggcgcag
     7621 cgcaaggccg gcatcggtca gtagcgacga aagggaaagc agagagtgag cacaagcgag
     7681 tacgtaggcc agcgccgccc cgtcaccgcg cttccgcggc cggagatcgt gctgcacggc
     7741 gtgcgcgatc gcccgcgcgg ctggctcgag tggctcacga ccaccgatca caagaagatc
     7801 gggatcctct acatctacgc caccttcatc ttcttcctca tcggtggcgt cgaggcgctg
     7861 atgatgcgcc tgcagctggc tgttccggag aacacgctgc tggcgccgaa ggcctacaac
     7921 gccgtgatga cgatgcacgg caccacgatg gtcttcctgt tcgtggtgcc ggtgctcgcg
     7981 ggcttcggca actacctcgt gccgctcatg atcggcgcgc gcgacatggc gttcccccgc
     8041 ctcaacgcgc tgtccttctg gttgctgctg ttcggcggga tcgtcttcta cgtcagcatc
     8101 ttcttccacc cgcccgaggc gggctggacg atgtacacgc cgctctcgga ggaggccttc
     8161 tcggcaacag gaggcgtgga cgcgtggatc ttcctgatcc acctgaccgg cctctcatcg
     8221 cttctgggcg cgatcaactt cgtcgcgacg atccacaaca tgcgcgcgcg aggcatgggc
     8281 tgggcgcgca tgccgctgtt catctggacg atcctgatct acagctacct gctgatcctc
     8341 gccctgccgg cggttgcggc ggccgtcacg atgctcctgg ccgaccgtca cctcgacacg
     8401 ggcttcttca acccggcgaa cggcggcgac ccgatgctgt ggcagcatct cttctggttc
     8461 ttcggccacc ccgaggtcta catcatgatc ctgccctcgt tcgggatcat cagcgaggtg
     8521 gttccggtat tcgcccggaa gccgatcttc ggctacaagg cgatcgccgc atccacggcc
     8581 gccatcgcgt tcctcggcat gctcgtgtgg gcgcaccaca tgttcgccac gccgatgccg
     8641 gcggtggtgc tgtccttctt catgctgagc tcgttcgcga tcggagtgcc gaccgggatc
     8701 aagatcttca actggctggc gaccatgtgg cgcggctcga tcgtcttcaa gacgccgatg
     8761 tacttcgcct gcggcttcat cgcccagttc gtgatcggcg gcatatcggg cgtgatcctc
     8821 gccgtgttcc cggtcgactg gcagctgcac gacacgtact tcgtggtcgc gcacttccat
     8881 tacgtgctct tcggcggctc gatcttcggc ctgttcgccg gcctgtacta ctggttcccg
     8941 aagatcaccg gccgaatgat gtcggagggc ctcggcaagg cgtcgttctg gctgatgttc
     9001 atcggcttca acaccacgtt cctcatccag cactcgctcg gcatgtcggg catgccgcgc
     9061 cgcatctaca cgtactaccc gaacggcgac tgggcggcgt ggaacctcgt ttccaccatc
     9121 ggctccttca tcctcgggat cggcgtgctc ctcacggtgc tgaacgtgct gttgagcctg
     9181 aagaacggca agcgcgccgg caacgacccg tggaaggcga atacgctgga gtggttcgtg
     9241 ccctcgcccc cgcccgagaa caacttcgac gtcgtgccgc gcattcgcag cgccgagccg
     9301 atgaaggaca tccgccgcga cgtggcggcc gccggcacgc ccgccggctc cgtgtaccag
     9361 cccgtagcgc cggggacgta gcttggaggc gggcgcccgc acgcgcgttg ccgtagcccc
     9421 gccgcgagtc ggctcgctcc ggcaggtcgt gtccgactac gtggacatga cgaagccgcg
     9481 cgtgcagtcg ctgcttctgt tcacgaccgt caccacgatg tacgtcgcgg gcacgccctc
     9541 gctctcgcgc gtcgtgctca cctgcctcgg cggcgcgctg tcagccggcg gcgccggtgc
     9601 gatcaaccat tacctcgacc gcgacatcga cgcccaaatg aagcgcaccg ccaaccgacc
     9661 ggtgccatcg ggcagagtcg cgccgggcgc ggcgctcgcc tggggcatct tcctcggcag
     9721 cgcttcgttc gtgctgatgg cgctcacgat caacctgctg gccgcctcgc tcgcgctcgc
     9781 tggcctgctg ggctacgtcg tcgtctacac gatctggctc aagcgaacca cgccgcagaa
     9841 catcgtgatc ggcggcgccg ccggcgcgat gccgccgctc gtggcctggg ccgccaccac
     9901 cggccacctc gcgggcgcgg cgctctacct gttcgcgatc gtcttctact ggacgccgcc
     9961 ccacttctgg gcgctgtctt tgctcatgaa ggacgagtac gcgagcgcgg gcgttccgat
    10021 gctgccggtc gttcgcggtg agaccgagac ccgctggcag atcctgctct acacgctgct
    10081 gctcgtgggc gtcacgctcc tgccggtcgc cttcggcgtc ttcgacggca tctacctgat
    10141 cgcggcgctg ctgctcggtt ccgccttcac ggcgatggcg atccgcctgc gccgcagcgc
    10201 cgagcgccgc gacgctctcc ggctgtatct cttctcgctg gcctaccttg cgctgctgtt
    10261 cgccgcgatg gtggccgacg cccgcctcta gcgcgcgtgc cgggcacgct cgccgagcgg
    10321 ctcacctccc aactgctcgc cggcccgccc gcgacgagtg ccgtggacgt ggctgagcgc
    10381 ctgctggcga tccaggcgca ggacccgcgc ggcgcgcgac tcgccgtgcg cgcacgcagc
    10441 gagggcgtag gcgcgtcgga tgtcgaccgt gccctcacgg acgagcgcac cctcctcatc
    10501 accacgctca accgcggcac gttgcacatg gtgcggagcg aggactatcc gtggcttcag
    10561 gccgtcacca ccccgccgct cgtccagtgg aacgcgcgcc ggctcgagca ggagggcctc
    10621 tcgcccgcgg actccgagcg cgccgtgacg ttactcgagc gctggctcgg cgaggacggg
    10681 ccgctcacgc ggccccagct gcgggagcga ctcaccgcgg ccggcatccg catccagggc
    10741 caagccctcc cccacgtcgt gatggcggcg tcgctgcgcg ggctcatcgt gcgcgggccg
    10801 atggtcggga agcagcacgc gttcgcgctc gtccgcaact ggctcggcga gcagcggccg
    10861 gtcgaccgcg acaccgctcg ggccgaactg gcgcgtcgct acctcgcggg ccacgggccc
    10921 gcgagcgacc gcgacctcgc gcgctggtcc ggcctgccat tgcgcgacgt ccgcgccggc
    10981 ctcgaggcga gcgcgcgcga gctcgtgcag cgcgacgacg gcctcctcga cttggcgggg
    11041 aaaccaaccg ccgcggatgt ccccgagccg cgcctgctcg gctcgttcga gcccgtgctg
    11101 ctgggttgga cgtcgcgtga cgacatcctg ggcgagaacg agggcgcgat cgtgcggggc
    11161 gggctcttcc ggcccttcgc catggtccgt gggcgcggcg tcgcgacctg gcgcctgaac
    11221 ggcaacagcg tcgagctcga gccgttcgag cccatctcgc gagcggacat ggccgcgctg
    11281 cggcgcgacg ccgggggggt cgagcgcttc ctcagtacct gacgtccgcg cttccctcgc
    11341 agtcggcgcg aatcgcgtcg aggaacgcaa gatacggctg cggaaggccg caggcgcgag
    11401 cccccgcgat caggccatcc acgtactcgc tcgacggcgt cacctccgcg ggctcctcct
    11461 tcacgacctt gtaggtgatc gcctcgaccg gctcctccgc gagcgtcagc accgtgaccc
    11521 gcatgcgctt gtacgcgtcg atcgtggtgc cctccttctc gtcgatcgtg gcgaggtccg
    11581 cctcgtcgat gtcgtagagc gcgccccaca ccgtcatccc atacgcgaag ctgatgtcgg
    11641 cgaccccggt cttcgtgcgc accgagcggc ggttgaacgc cagtcggtga tcggtcaggc
    11701 gcgccggccc gacgtattga cagtccggcg agtacgcggc caggcgctcg ggagccatgt
    11761 tgctcccgta cgcgaagtac ttgactcggc ccgagcctga cttcacggcg agagtctacg
    11821 tcgggccatt ggcagctgcg tcctgaaggc ccacagtcac aaactggtaa tcggcggaga
    11881 ggcaaccggt accccacacc ttcgcgcccg gcgaaaccgg gtagaccaga attcaaggag
    11941 gggcaagcga atgcctacta cagaggaact cgcgagctgc cgtggccagc aagtggtcgg
    12001 cccgagcggc gacaagatcg gcaagctgga ggaggtctac gtcgactccg cgaccggcaa
    12061 gcccgaatgg ttcgccgtta gcaccggcct gttcggcacc cggctcagcc tgattccgat
    12121 cgccggggcg aacctgcgcg atgacactgt gcaggtggcc tacgacaagg ccaaggtaaa
    12181 ggatgcgccg aacgtggatc cggacggcga cctctctcag gaccaagagg cgcggctgta
    12241 tggccactac ggactcgcgt actcc
//
LOCUS       DASVXS010000033         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_150890, whole genome shotgun sequence.
ACCESSION   DASVXS010000033 DASVXS010000000
VERSION     DASVXS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150890"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..934)
                     /locus_tag="VF752_01690"
     CDS             complement(320..934)
                     /locus_tag="VF752_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8854284.1"
                     /translation="MLRLGETERIEIAGGIAWRPLRRPLGVTAFGANAYTADAGEEVI
                     EEHDETGAGAGGHEEMYVVMSGRAQFTIDGEEIDADAGTFVFIPERSARRHAVASEDG
                     TAILAIGGPAGAALPVSPWEYWMAAEPAYQAGDYERAIEIASEGLEDYPDHGHLHYQL
                     ACYHALAGHMDEALQHLRRAYEIEPKTREWAAGDSDVDSVRDLV"
     gene            complement(954..2030)
                     /locus_tag="VF752_01695"
     CDS             complement(954..2030)
                     /locus_tag="VF752_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX8854285.1"
                     /translation="MTVATSARADRLVDQLADRELDAIIVTNLVNVRYLTGFTGSNGA
                     AIVSPDMRVFITDFRYVEQAEKQVPDFELARGKQDLLSDVVKRLSGRVGFEDTHVTVR
                     QYERLQELVPEGVELVGAGGAIEELRAVKDEEELRLIRNAAELSDQIFQYIASGPIVG
                     RTEREVAVDLEHWMRQHGAERPSFPSIVASGPHGALPHAEPRDEPIVADTLVTFDLGC
                     VVDGYCSDCTRTFATGEISDEMREVYELVRSAQLTALDGVRAGAENRAVDTASREPIA
                     AAGHGDHYGHGLGHGVGLEIHEAPRLAQTAEGTLDAGNVVTVEPGVYVPGKFGVRIED
                     LVAVTDDGAEIFTHFPKDLITLGS"
     gene            complement(2027..2467)
                     /locus_tag="VF752_01700"
     CDS             complement(2027..2467)
                     /locus_tag="VF752_01700"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX8854286.1"
                     /translation="MSNRVGVMHGVNLDQLGRRDPGHYGDNSLMELERLVRRFGNELG
                     LEVAFSQTNHEGEFCEMLHRAPERADGLILNPGAWTHYSWAIHDALEIAALPAVEVHL
                     SAIDEREEWRRFSVIRDLCIGSVQGKGVDGYREALEMLKEKLDT"
     gene            complement(2464..>2754)
                     /locus_tag="VF752_01705"
     CDS             complement(2464..>2754)
                     /locus_tag="VF752_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854287.1"
                     /translation="RRYRHGEAIAIGLLAALRLSGRGSLRAEVADLLAAHGLPTRLEG
                     ATPEQVFELVGRDKKRSGGRVPFVLVQSPGDVTHGHELPDAEVLGAIQELAA"
BASE COUNT          398 a         1006 c          910 g          440 t
ORIGIN      
        1 gcccccgtag tgctcgcggc cctcgccatc gccccacgtg gtgaacggct cgggctcgat
       61 ttcgaagctg ccacgacggc gttcgcggcg gccagtccag gcggctcatt acgatcgccg
      121 gcccccagaa cgcgggcgcg aaactcgaac cgctgtcgcg gacgggaccg gtggcggagg
      181 aggatcacga gctcggatcc gtccgccctg atcccctcca gttgcacgcc gtcgcgaccg
      241 agcgcctcag tggcgaatgg gcccagcgca cggacaaact cagcgagcca cgcagcgctg
      301 tccaatccgt cgccgcccac tagaccaggt cgcgcacaga gtcgacatcg gaatcgccgg
      361 ccgcccactc gcgcgtcttc ggctcgatct cgtaggcgcg cctaaggtgc tgcagcgctt
      421 cgtccatgtg tcccgcgagc gcgtggtagc aggccagctg gtagtgcaag tgcccgtggt
      481 cgggatagtc ctcgagcccc tcgctcgcga tctcgatcgc ccgctcgtag tcacccgcct
      541 ggtacgccgg ctcggccgcc atccagtact cccacggcga caccggtagc gccgccccgg
      601 ctggcccgcc gatcgcgagg atcgcggtgc cgtcctcgct cgcgacggcg tgtcgccgtg
      661 ccgaccgctc ggggatgaat acgaaggtgc cagcgtcagc gtcgatctcc tcgccgtcga
      721 tcgtgaactg tgcccgaccg ctcatcacca cgtacatctc ctcatggccg cccgcgcccg
      781 cgccggtctc gtcgtgctcc tcgatgacct cctcaccggc atcggccgtg tacgcgttgg
      841 cgccgaaggc agtcacgccg aggggacggc ggagcggccg ccacgcgatg cctcctgcga
      901 tctcgatgcg ctcggtctcg ccgagtcgca gcaccttgaa cggggcggcc gttctagctc
      961 cccagggtga tgaggtcctt cgggaagtgc gtgaagatct cggcgccgtc gtcggttacc
     1021 gcaacgagat cctcgatgcg cacgccgaac ttgccgggga cgtagacgcc cggctcgacc
     1081 gtcaccacgt tccccgcatc gagcgtgccc tcggcggtct gcgcgagccg cggcgcctcg
     1141 tggatctcga ggcccacgcc gtgcccgagg ccgtggccgt agtgatcgcc gtgcccggcc
     1201 gcggcgatcg gctcccgcga cgcggtgtcc accgcgcggt tctcggcgcc ggcccgcacg
     1261 ccgtcgagtg cggtcagctg cgccgagcgc accagctcgt acacctctcg catctcatcc
     1321 gagatctcgc cggttgcgaa cgtgcgcgtg cagtccgagc agtagccgtc aacgacgcaa
     1381 ccgaggtcga aagtgacgag cgtgtccgcg acgatcggct cgtcgcgtgg ctccgcgtgc
     1441 ggcagtgcgc cgtgcggccc gctcgcgacg atgctcggga aggagggccg ctccgcgccg
     1501 tgctggcgca tccagtgctc gagatcgacc gcgacctcgc gctcggtccg cccgacgatc
     1561 gggcccgacg cgatgtactg gaagatctgg tcggagagct ccgccgcgtt gcggatcagc
     1621 cgcagctcct cctcgtcctt caccgcgcgc agctcctcga tcgcaccgcc cgctcccacg
     1681 agctcgacgc cctcgggcac cagctcctgc aggcgctcgt actggcgaac cgtgacgtgc
     1741 gtgtcctcga agcccacgcg cccggacagg cgcttcacca cgtcgctcag aaggtcctgc
     1801 tttccgcgcg ctagctcgaa gtcgggcacc tgcttctccg cctgctcgac gtagcggaag
     1861 tcggtgatga acacgcgcat gtccggcgac acgatcgccg cgccgttcga gccggtgaat
     1921 cccgtgaggt agcgaacgtt cacgaggttc gtgacgatga tcgcgtcgag ctcgcgatcg
     1981 gcgagctggt ccacgaggcg gtcggcgcgg gcgctggttg caacggtcat gtgtcgagct
     2041 tctccttcag catctcgagc gcttcacggt agccgtctac gccctttcct tgcaccgagc
     2101 cgatgcagag gtcacggatc accgagaagc gccgccactc ctcgcgctcg tcgatggcgc
     2161 tgaggtgaac ctccacggcg ggcagcgcag cgatctcgag cgcgtcgtgg atcgcccacg
     2221 agtaatgagt ccacgcgccc gggttgagaa tgagcccgtc cgcgcgctcg ggcgcgcggt
     2281 ggagcatctc gcagaactcg ccctcgtggt tggtttggga gaacgccacc tcgaggccga
     2341 gttcgttacc gaacctccgc acgaggcgct cgagctccat gagtgagttg tcgccgtagt
     2401 gcccggggtc gcggcggccg agctggtcga gattcacgcc gtgcatcacc cccacccggt
     2461 tgctcatgcg gcgagctcct gaatcgcccc tagcacttcc gcgtcgggca gctcgtgacc
     2521 gtgcgtcacg tccccgggcg actgcacgag cacgaacggc acgcggccgc cgcttcgctt
     2581 cttgtcgcgg ccgacgagct cgaagacctg ttcgggcgta gcgccctcca agcgggtcgg
     2641 aaggccatgc gcggcgagga ggtcggccac ctcggcgcga agcgagccgc gccccgacag
     2701 ccgaagggcc gcgagcaggc cgatggcaat cgcctcgccg tgcctgtagc ggcg
//
LOCUS       DASVXS010000034        30172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_996291, whole genome shotgun sequence.
ACCESSION   DASVXS010000034 DASVXS010000000
VERSION     DASVXS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 30172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30172
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996291"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF752_01710"
     CDS             <1..247
                     /locus_tag="VF752_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854288.1"
                     /translation="RLERAAELLAHRPWRFCAAFGLLAGLVVAAGHAIGDGALPLRAG
                     ALAAGAALTGIELLAVAVGFATLGRWLRLWSPSDHRA"
     gene            complement(228..509)
                     /locus_tag="VF752_01715"
     CDS             complement(228..509)
                     /locus_tag="VF752_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854289.1"
                     /translation="MWGYPQRRSRAQTAGDVHEEGRWSHDSGARSGWRPRCRPSRRPY
                     YRSRLDIGLSQGGRADCEDLRAGCAQLVTDDGEPADYSYLAAALTPDGR"
     gene            567..1871
                     /locus_tag="VF752_01720"
     CDS             567..1871
                     /locus_tag="VF752_01720"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX8854290.1"
                     /translation="MSTDTATTTVLRPTGGGGVRAVLRAPFEARTWRASAYLLLSFPI
                     GVAAFVVLVTGFSLAFGLLITLIGIPILLGTLAVARGFAMVERRRAAVLEGTPIPDPY
                     RPIPEGRIFGRLWAWLKQASTWKDVAWLFLLFPLGIFDFTLVVTVWAVAIGMLTLPAW
                     FWAVNSDPDSLDFGLFHANTLPKALIVAAVGLLATLLVPWIVRGWAHVELLMQRGLLG
                     PGREELEQRVGELTATRAGAVEAQASELERIERNLHDGAQARLVALAMDLGMAEEKLD
                     SDPDHAKQLVGEARSQAKQALTEMRDLVRGMQPHILRDRGLDAAIPALAGSTRLHTTV
                     RVDVPRRLPAGVEAAAYYVVSESLANVAKHSGSQLAAIDVALNGDWLVVQVTDQGSGG
                     ADQNGGGLAGLAKRVRALDGTFDVTSPPGGGTRVRAVIPCAS"
     gene            1859..2503
                     /locus_tag="VF752_01725"
     CDS             1859..2503
                     /locus_tag="VF752_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854291.1"
                     /translation="MRVVIAEDLALLRDGLTRMLRDNDMEVVAAVEDGTSLVESIVEH
                     KPDVAVVDVRLPPSFTDEGLRAALEARQKVPGTAVLVLSQYVEQTYASELLAEGSGGI
                     GYLLKERVADVRDFVDAVRRVAEGGTAMDPEAIAQLVAKRGADGPLEELTPREREVLG
                     LMAEGRSNAAIAEELVVTEGAIEKHISNIFWKLSLPPSDTHHRRVLAVLAYLRG"
     gene            complement(2492..2896)
                     /locus_tag="VF752_01730"
     CDS             complement(2492..2896)
                     /locus_tag="VF752_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854292.1"
                     /translation="MLERLWRPPLFAELPAAIKVVNAGVLPIVSGAATGLLLGVSGTW
                     YVVANVLAAIGGFAAGREHVTPRTGALRGTWGGLLFGSALLIAHELADNGEEIQALPH
                     PPVLLLVFTIAIGALLGALGARSRGAEQALPA"
     gene            complement(2901..3467)
                     /locus_tag="VF752_01735"
     CDS             complement(2901..3467)
                     /locus_tag="VF752_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8854293.1"
                     /translation="MSKTQYYCASSLDGYIAEADDTLDWLTGYEGSSAVEGAEPGGRG
                     SYERFFENVGAMISGSVTYEWVLDHVDEWPYPGKPFWVLSTRELRVPEGNAVDVRIVN
                     AKVPEIYDEMAGAAGEGNLWVIGGGNVASQFADEGLLDEVIVTVVPVVLGSGKPLFDR
                     RVPGPPLTLTGTTAFDSGMVELRYEVTR"
     gene            complement(3480..3971)
                     /locus_tag="VF752_01740"
     CDS             complement(3480..3971)
                     /locus_tag="VF752_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HEX8854294.1"
                     /translation="MAAAGAAPTAASRRSSRRRSVIVQERLDKVVVHVDGGARGNPGP
                     AAVAAVATATDGSVLAERNLYIGEATNNVAEYRAVLLGLELARELGANEVEVVNDSEL
                     VARQIGGQYKVKHAGLRPLFVETMNQLREFESWSVRTVRRESNERADELVNEALDEAL
                     GAG"
     gene            complement(3913..4113)
                     /locus_tag="VF752_01745"
     CDS             complement(3913..4113)
                     /locus_tag="VF752_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854295.1"
                     /translation="MIYCVVPQALADELYDRLVEYYRDDPNVEVIIDRRKQGGDPTNP
                     QPPDRRRRRRAYGSFPSIEPPA"
     gene            complement(4110..4847)
                     /locus_tag="VF752_01750"
     CDS             complement(4110..4847)
                     /locus_tag="VF752_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854296.1"
                     /translation="MPRELRPTHDVVTCDVCGRTILKGERAEPYLVPGGQRRLVCDLC
                     IGRAQHEGWIRESAHGDMPTAHPRREPRRGLLGRLRGRREDNGHPEPEWAPPPALAYD
                     DEHQPAAEAAPEPPPDRKRTRAWRPKDPRHVRAVPTNAQVKVERALEVFNGSEHPRTI
                     LGIARSLGDPWVYAAPLETKPSEVEIVVAWELSWYRYRVDLGDADDPVTMLEKGEDLD
                     QIDEQLRAWNAAMTADGRLAVGVGSAE"
     gene            complement(4915..6990)
                     /gene="fusA"
                     /locus_tag="VF752_01755"
     CDS             complement(4915..6990)
                     /gene="fusA"
                     /locus_tag="VF752_01755"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8854297.1"
                     /translation="MPHESAKRIRNVALVGHRGSGKTSLNEALLFEAGALNRLGSVAD
                     GTTVSDSAADEKSRQMSITSSLSSLHWEDRKINLIDTPGEPSFVADALAALRVSEGAV
                     FVVNAVMGAEVGTARLWDRARELGLARVVFVNMLDRERADFFRTLESLKEAFGPHVVA
                     TEIPIGMEHEVRGVIDLVDMKAYEYEGSDRENCREIEIPEELQDQAQEYREKLLDEVA
                     ENSDELMERYLEGEEISHEETVEALKAGVTAGRLFPVTCGVATHNLGTNRLLDAFVED
                     LPSPGKKGPVQAGDITLEPSEDGDLVAYVFKTLADPYTGRINLFRVYQGVINADSQVY
                     NARSGQKERIGQLLEIQGKESQQATDFGPGDIGAVAKLKDTHTGDVLSSSDAKIELPL
                     PAMPAPVMAFALEPKSKGDEDKVFSALRRLQEEDPTIDVHRDPQTGEQILAGLSQMHV
                     EVIVDRLHERFGADVNLKPPRVPYRETIKTAAKAHGRYKKQTGGRGQFGDCHIEIEPL
                     PDGQGFEFVNAIKGGVIPSGFIPAVEKGIAEAMRDGVVAGYPMSGVRVRLYDGSYHSV
                     DSSEMAFKVAGSMAFKEAAGNAGAVLLEPIMTVTLSVPNDSVGDVIGDLNSRRGRPLG
                     MDPKGSVTEVKAEVPMAEMLSYAPDLRAITGGQGDYTMDLARYEEVPQHLAQKVIQAA
                     SQEAEAVKA"
     gene            complement(7261..8190)
                     /gene="whiG"
                     /locus_tag="VF752_01760"
     CDS             complement(7261..8190)
                     /gene="whiG"
                     /locus_tag="VF752_01760"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX8854298.1"
                     /translation="MHPRAARPCDDQHNPDLHSGRVETPETGLRSGSPTSLSFRISGD
                     ILETNVKEIELRELWRRYKDDADKAARERLVLAYSPLVKYVAGRMSTGLPSHVEESDL
                     ISYGLLGLISAIERFDPAREIKFETFAITRIKGSIIDELRSLDWVPRSVRAKAREIEK
                     VNAALEHKLHRAPTDQEMAEALETTVEDFQASLTRISNSSVVALDELWTLSDASGDQV
                     SLLDTIQDPQATDPAHAMDTSETKDRLADAIARLPEREKLVIALYYYENLTLREIGEV
                     LGVTESRVSQLHTKAVLRLKSRLQTDMLEDAAG"
     gene            complement(8079..8993)
                     /locus_tag="VF752_01765"
     CDS             complement(8079..8993)
                     /locus_tag="VF752_01765"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX8854299.1"
                     /translation="MDEISPPPSWADALRGFDRALRAHGMAEKTRRAYGTDLEQLAEW
                     ASRSGLEPKGIGPRELRRYAAVLSERGASKSTVARKLAAVRSFYRQMVERGELEASPA
                     DLVASPKRDAYLPRVLRPGEVAQLLERIPAATPLELRDRAMFELAYAAGLRAEEIVNL
                     DLTDPDADGEELRVQGKGGRTRIVPAGELAWKALESYLDRGRPALAVEGARVEPALFL
                     SKTGQRLSTSDVRRRLGLWTRRAALAGGVSPHTLRHSFATHLLEGGADLRSIQELLGH
                     ATISTTQTYTRVESKRLKRAYAQAHPRA"
     gene            complement(9039..10079)
                     /locus_tag="VF752_01770"
     CDS             complement(9039..10079)
                     /locus_tag="VF752_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEX8854300.1"
                     /translation="MRHRSFIALAVALVVMIGGAVAVYAYDSTRTDKVAKGISVSGID
                     LGGMTRSQARTALDQQLAAPLSRPLVVKYGRERFRLSARRAHLRTNVDAIVDEAMRRS
                     RQGNIVTRSWRSLTGGEVHAQLTPEISYSHAAVERLVRRVKSQLDRPAVDATVSFTSG
                     AFQPVPSHQGNAIRGVELTRNIEAALVMPDGDRVVRAHSKITKPKVTTDQLAQRYPAI
                     IIVDRNAKQLKFYRHLSLNHTYRIAVGMVGLETPAGLYHIQNKQINPPWNVPHSSWAG
                     KLAGKVIPGGAPNNPLKARWLGIFNGAGIHGTDNIASLGTAASHGCIRMSIPDVEQLY
                     PHVPVGAPVYIS"
     gene            complement(10209..11585)
                     /locus_tag="VF752_01775"
     CDS             complement(10209..11585)
                     /locus_tag="VF752_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8854301.1"
                     /translation="MNSKRVLVTGISTYWGGRLAQALERDSEIEAIVGVDSRDPTREL
                     DRTEFVKVGTQHALLRRIVEAAEIDTVIDTRLVPDSSVLKPSAAHENNVIGTMNILAA
                     CSGPNSTVRKFVFKSSAHYYGTAQDDPAFFTEAMRRPHPPTTPIERDIVEAEAAVADF
                     ADKNEDVSVTVMRFANVLGPDVETSHTKLFSLPAVPMILGFDPRYQFVHEDDVVAALK
                     HGVEFELRGIFNVAADGVLALSEVAGLLGRPYAPILPPWGTGLAANVLSRLGVRVPPE
                     MLSQLRFGRGLDNRKLKATRFEYRCTTRETVAKFAEYLRLSPILRGVQEPYRYEREVE
                     DFLRWSPHVKNPAYRREGTLDPRQAFQLRKFLEEYDEGNGQSEAPPRAVRRSRTPKAK
                     PAEPPAPPVAHYDDLEAEEVISLLGSLELADLEALREYERRAQARQPVIAAIESILAR
                     RAASSSHP"
     gene            complement(11582..12490)
                     /locus_tag="VF752_01780"
     CDS             complement(11582..12490)
                     /locus_tag="VF752_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8854302.1"
                     /translation="MADSTRTSLRPSEELVARPASGEGLPAPADADLRRYLPAIEPER
                     RLNDWGRSERVEGVFDRYLLEFFYRYWFRVEVEGIENVPDTGGGLLVSNHAGALPPDA
                     AMIGKAIKEEHPHPRPLNVTVEHFFKGYPGFSMLIPKIGCVAAHPANVHRLLYDEEQL
                     VLVFPEGRKGTEKLYKDRYKLRRFGRGGFVEAAMRARAPIVPVCVVGAEEAAPIFAQV
                     GLLRRLTGLIYFPITPTFPHLGPLGMLGYLPAKFRIRFLEPIEFPDEDMADDRGLVQS
                     YAHDIRARIQENVFEMVEQRESVWFG"
     gene            12581..13297
                     /locus_tag="VF752_01785"
     CDS             12581..13297
                     /locus_tag="VF752_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX8854303.1"
                     /translation="MRSKDEVRREVWRAMDREGVSRFPGAEGRIPNFAGAKAASERLA
                     RHPDWVRAETIKANPDAPQTHSRRLALAQGKCLVMAVPRLRDEHPFRLLDPRRLSEEQ
                     QREAATIKGALRHGKVIDLEQVPPLDFILTGSVAVNLKGVRIGKGGGFSDLEYGLLAE
                     SGKVDRRTVVATTVHPIQIVRENLMVTGHDIPVDIVSTPRAVIEVERAHERPTGILWD
                     HLQPPQIHEIPVLERMGYAG"
     gene            complement(13294..13878)
                     /locus_tag="VF752_01790"
     CDS             complement(13294..13878)
                     /locus_tag="VF752_01790"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854304.1"
                     /translation="MAKIALDIDSTLHHYWDVFDAIVQRRFGVELPYEEQTDWGIALL
                     ERDQLIACVEETHSVENIMGAKPYPHAVETVRAWHDAGHFIHVTSHRAESAREATEGW
                     LDEIGLPYDDLHCSYDKVSRCVELEIAVLVDDSPVNIQRALEVGIVPATLIHPWNAHL
                     ADDERVIVSEDWRALRDRVAPLLSSIDAEEPAPS"
     gene            13900..14895
                     /locus_tag="VF752_01795"
     CDS             13900..14895
                     /locus_tag="VF752_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8854305.1"
                     /translation="MPIFPSKARPASLRAARRADSDYGVGASPNWRDIDWREHLRSTE
                     IDGRRVNYVDIGSGDGPPVVFIHGLGGAWQNWLENLPRVAQERRAIAVDLPGHGQSGM
                     PAEKVSISGYGRCVDALTEQLGFSEVALVGNSMGGFIAAEMGIQFPERVAQIVLVAPA
                     GITTTELRRAPALTWARTAAALGAWASTRSEIFVKRPALRHVALQIVTRHPSRIAPDM
                     AWEMIAHSGKDGFVDATRALLEYDFRDRLPEIRCPTLIVWGDRDALLDVGDADEYARL
                     IPDSRKVLMQDTGHMAMLERPVAFNDCLLEFLHGQIDTSGDSDAERAAAGEEAAA"
     gene            complement(14899..16572)
                     /gene="ilvD"
                     /locus_tag="VF752_01800"
     CDS             complement(14899..16572)
                     /gene="ilvD"
                     /locus_tag="VF752_01800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX8854306.1"
                     /translation="MSVDIRRRSRAILEGPDRAAARAYLKGIGFDDDALKKPIVGVAN
                     TWTETMPCNFHLRRISERVKAGVREAGGTPMEYNTVAISDGITMGTEGMKTSLVSREV
                     IADSIELVARGHMFDAVIAISGCDKTIPGTVMALARLDIPSLMLYGGSILPGCFRGQD
                     VTIQDVFEAVGAHAAGSMSDEELQELEDVASPGPGACGGQYTANTMAGVFEMMGISPM
                     GSNSVPAVDEHKGDVAERAGQLVLDLLERDVTARSLITRESLENAIASVAATGGSTNA
                     VLHLLAVAHEAGVPLDIDDFDRIAWKTPLLADLKPGGRFTAPDMHAAGGMRVVANRLS
                     EAGLLQEDAPTVSGKTIGEEADAANETEGQEVIRPLSNPIQPTGGFAILRGNLAPEGC
                     VVKLAGHDRREHRGPARVFEGEEEAFAAVKAQQIQPGDVVVIRNEGPSGGPGMREMLA
                     VTAALVGEGLGESVALLTDGRFSGATHGFMAGHVAPEAPRGGPIAAVEDGDIVVFDVP
                     DRRLDVELDQDEIDRRVAAYEPKPSAYEGGVLGKYARHVGSASRGAVTT"
     gene            complement(16569..17204)
                     /locus_tag="VF752_01805"
     CDS             complement(16569..17204)
                     /locus_tag="VF752_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854307.1"
                     /translation="MLLALLALTLVAVVLALPVLVQAWSSWRREHGPLFRTRRVERVM
                     LHDPGRERRAEQRARELLRSCVNDEEWSMYRDLGFIKVNGKLADGSAPRRPREAADAP
                     EPEAPYAYLIYPHKPIVAFVPKTRRLLSEYCVEFPDLTRAISHSRLPDSDDVLAKWMA
                     LTGDERNLIRNANMHLPGRQIDPGRVRRDLWRLAEWERQRRSSGSYEARRR"
     gene            complement(17223..17456)
                     /locus_tag="VF752_01810"
     CDS             complement(17223..17456)
                     /locus_tag="VF752_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854308.1"
                     /translation="MKPGFGEVLLTEGDPRVREDEERLVEEFRRQLDEGMWAAVPTVG
                     EDSGRREAQMVQRYADIPTDAERVIFFPRASGG"
     gene            complement(17586..18392)
                     /gene="thiD"
                     /locus_tag="VF752_01815"
     CDS             complement(17586..18392)
                     /gene="thiD"
                     /locus_tag="VF752_01815"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX8854309.1"
                     /translation="MQSGARRIPRVLTIAGSDSGGGAGIQADLKAFAHAGVYGMSAIT
                     AITAQNTVAVTGIYPLPPEAIVDQVRAVAEDIGVDAVKIGMLGNTETIEAVARALDLV
                     GQAPTVLDPVMVAESGAELLEPGARDALRALLVPRVTAITPNLPEARLLAAAPGGAPE
                     DLARALQALGPEFVVVTGGHRDQAVDVFFDGATLAEIAGERYDSGAAHGSGCTHSSTL
                     AAQLALGRTPLEAAREAKRVASAAVRDGLDEIGAGAGPVDVLGLARRGGD"
     gene            complement(18414..19529)
                     /gene="dprA"
                     /locus_tag="VF752_01820"
     CDS             complement(18414..19529)
                     /gene="dprA"
                     /locus_tag="VF752_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX8854310.1"
                     /translation="MSACDACLRRSHLIAFLAARVAAVLAEPRPRPSAILGLPELEFI
                     EAVAGGRKDAAERFIEDFDADVARNMAERAGIGELCAHDPLYPERLGDLYDPPAVLYH
                     TGSAARLAQITELPAVTIVGARRASSAGVAMAHELGRGATTAGITVVSGLALGIDGAA
                     HRGALDAAGAPLAVLAGGADIPYPRNHVRLYEQVRERGAVVSELPPGVRPFRWSFPAR
                     NRIMAALGRVTLVVEARDRSGSLITADFATQLNRGLAVVPGPATSPFADGCLKLLRDG
                     AASVIRGVADLVDDVYDGEARPEGWDPSGRAYVLEQLSDFDRTVLDAVEEGQHAGAIA
                     REHAVGAGRVRAALARLEMAGLIVPDGRGAYQPSPAR"
     gene            complement(19526..21034)
                     /locus_tag="VF752_01825"
     CDS             complement(19526..21034)
                     /locus_tag="VF752_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX8854311.1"
                     /translation="MVSRVATFAIEGVEGREVTVEVDVRSGLPVFTIVGLPDAAVREA
                     RERVRAAVLNSGLEFPMRRLTANLAPADIRKAGPGFDLALAVALLGSSGQLPGVDLDE
                     WAFCGELALEGYLRPVRGIVAMAAGARRSGRRRIVVPTENAAEAAVIEGIEVYGLHRL
                     RDIGALLAGELGVSPAVPAVPAQVDRSYGPDLAEVRGQEDARRALELAAAGGHNLLMF
                     GPPGSGKTMLARRLPGILPPPSLEEALEITRIHSVAGKSDGTLARRRPFRAPHHTVSA
                     SGLIGGGSTPRPGEVTLAHRGVLFCDELAEFSRPSIEALRQPLEDKRVEIVRGQQSLT
                     FPASVMFVAASNPCPCGRSLAKCECTETDVRRYQRRLSGPLLDRLDLVCQVQPPSDAP
                     GEGGESSEVVAARVAAAREWQTQRLSGSAAMCNADMDVRETRRHAALDPAAARRLAEA
                     VVAGAISERGHDRVLRVARTIADLDGRDAICDEDVDEALAYRILPPSMAVAA"
     gene            21164..21544
                     /locus_tag="VF752_01830"
     CDS             21164..21544
                     /locus_tag="VF752_01830"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX8854312.1"
                     /translation="MASDPRRRLGAIGEDLAARHLECAGYEVLERNARTRTGEIDIVA
                     ARAQVLVFCEVKTRVTRSTGGPTGPFAAIGPRKRQQVRLLAREWLAGASGRPHYPDLR
                     FDAIAVALTASGELVALDHLEDAF"
     gene            complement(21550..22131)
                     /locus_tag="VF752_01835"
     CDS             complement(21550..22131)
                     /locus_tag="VF752_01835"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX8854313.1"
                     /translation="MAGADEAGRGSLAGPLVTAAVLFDLERLTLSDRRALSRLNDSKQ
                     HDEEGREELYPIVMRAAAKVSIVSRCVRGIDARGLHVTNIAALRAALMRVACPGSICL
                     VDGFKLPDFGYEQRAVIGGDSRSAAIAAASVLAKVTRDRYMRRAHERHPDWEFDSNVG
                     YSTPEHRAAIAANGICPLHRMSFASIAYQQLAL"
     gene            complement(22193..22822)
                     /gene="lepB"
                     /locus_tag="VF752_01840"
     CDS             complement(22193..22822)
                     /gene="lepB"
                     /locus_tag="VF752_01840"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8854314.1"
                     /translation="MSRPARVPGSRKSDSKTGSLVELVTIVAVALGLALLIQAFIVKP
                     FRIPSESMVPTLAVGQRVLVDRVTLHFSDPHRGDILVFKPPKGADTDSCGKAMPANTA
                     CDTPTAQHSDTNFIKRVVGVPGDKLFVKAGRVYINGKLQKEPFIRPDANCEICNETNP
                     ITIPPGHFFMMGDNRGQSADSREWGPIPKKWIIGEAFFTYWPPKRIGTL"
     gene            complement(23163..23495)
                     /gene="rplS"
                     /locus_tag="VF752_01845"
                     /pseudo
     CDS             complement(23163..23495)
                     /gene="rplS"
                     /locus_tag="VF752_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(23610..24284)
                     /gene="trmD"
                     /locus_tag="VF752_01850"
     CDS             complement(23610..24284)
                     /gene="trmD"
                     /locus_tag="VF752_01850"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8854315.1"
                     /translation="MNIDVFTLFPGWFDWFREQRHVRNAEALGHRLEAIDMRATTPLG
                     AGQVDDTPYGGGAGMVIRVDVVEAALRARYGHDPLELPKERRVIALAASGRQFDDELA
                     TELAAEPELTVLCGRYEGFDQRVHDHLASDVLSIGPYVLAGGELAAMVICDAVIRKLP
                     GALGHEDSAVEESFSPALEGGPEYPHYTRPAEWHGHAVPEVLVSGNHARVREWRLEQS
                     RRRGGR"
     gene            complement(24281..24775)
                     /gene="rimM"
                     /locus_tag="VF752_01855"
     CDS             complement(24281..24775)
                     /gene="rimM"
                     /locus_tag="VF752_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX8854316.1"
                     /translation="MTEQRHVAVGRVGRAHGRDGSFWVEAPEHALDVGTDVRIGGVSG
                     RVERRDGDAERALLRVSGVDDRDAAAALSGEPIEVAESELPLAEDEWLVEDLLGCKVP
                     GIGEVVAVLAGPSCDLLEVGDRGVLVPLVSDAVREVDLERRVIEVDHDFLALSDQEQE
                     GGGA"
     gene            complement(24772..25005)
                     /locus_tag="VF752_01860"
     CDS             complement(24772..25005)
                     /locus_tag="VF752_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX8854317.1"
                     /translation="MEDLLEYLARSLVDEPDEVSVEAFEEDDGTVVLELSVGEDDVGK
                     LIGRGGRTINAVRTVIRASAVKHGCRVLVDVVD"
     gene            complement(25013..25270)
                     /gene="rpsP"
                     /locus_tag="VF752_01865"
     CDS             complement(25013..25270)
                     /gene="rpsP"
                     /locus_tag="VF752_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX8854318.1"
                     /translation="MRLTRVGSKKNPIWRVVVADQRSPRDGRVIETIGRYNAQVEPSE
                     IVLDQERLRHWLDRGAQPSDTVKKLMRATGFQQAGAGDSTG"
     gene            complement(25324..26652)
                     /gene="ffh"
                     /locus_tag="VF752_01870"
     CDS             complement(25324..26652)
                     /gene="ffh"
                     /locus_tag="VF752_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX8854319.1"
                     /translation="MFEALSERLEAALDDVRSRGKLTEDDVNKAMRQVRLALLEADVN
                     FQVVKRFTADVKERALGADVLQSLNPGQQVVKIVGDELTELMGGAGRDLVISPRAPTV
                     ILMAGLQGSGKTTATAKLARHLQEEEGKSVALVAADVYRPAAVDQLVKLGSQVGADVY
                     EQGTDRDPVDIAEWAVNRAKSEGKDVVIIDTSGRLHVDQALMDELAKIRKRTKPHDVL
                     LVVDAMTGQDAVNVAEQFAEVANFDGVVLSKLDGDARGGAALSVKAVTGKPILYASTG
                     EKLEQFERFHPDRMAQRILGMGDVRTLIEKAEQQMDERQAEELERKIRKQQFGLDDFL
                     DQLHQLRRMGPLTSIMKMIPGMGSQLGNLKVDERELDRIAAIITSMTPEERANPALLN
                     GSRRKRIAQGSGTNVSAVNQLVKQFGQMQKVMRQLSQGKMPSLQQLARGR"
     gene            complement(26682..27806)
                     /locus_tag="VF752_01875"
     CDS             complement(26682..27806)
                     /locus_tag="VF752_01875"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF005758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HEX8854320.1"
                     /translation="MAREDFGDGTRAVRAGLPEAGQGEPFLPGPTFAAPYHLRGRPED
                     SDFVYGRYGNPTWSRYEAALAELEGGAAAVVFASGMAALSALLLPRLRPGDVLVAPAD
                     SYRGTRILAERYLQPMGVEVRLAPTPGDAMAAQLDGATFLLLETPTNPQLEVCDIRAL
                     ADAAHERGALVAVDNTFATPLGQRPLELGADFSVASASKMLTGHADLILGYVATRDSA
                     HPAITAWRTMAGSVPGPFEVWLAHRSLATLGVRLERQCATALALAQALAERDDVSAVR
                     YPGLEGDPSHELAARQMTRFGPILSFDVGSQERADRFLAECRLVSDATSFGGVHTSAE
                     RRARWSGDAVSEGFIRFSAGCEETADVVGDVLRALDTARS"
     gene            complement(27809..28801)
                     /gene="ftsY"
                     /locus_tag="VF752_01880"
     CDS             complement(27809..28801)
                     /gene="ftsY"
                     /locus_tag="VF752_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX8854321.1"
                     /translation="MPRTWTELFVTGDAPAPETDASEAQEERRGFFRRLRDNMSKTRQ
                     ALGAELQASVFQNLDDEAWERLEETLIYADVGAPTTARIVERLETEAASGDLSTGEEL
                     SDRLRELLAESARTGDATIDLRHDPTVILMVGVNGTGKTTTIGKIAWHLQNELGKSVL
                     LAAGDTFRAAAVEQLTAWAERAGTDIVKAAQGSDPGAVAYDAIEAARARGHDVVIIDT
                     AGRLHTQDHLMDELAKVRRVIQKLIPDAPHETLLTIDATTGQNGLRQALLFREAVDVD
                     GIVLTKLDGTAKGGIALAIAQELGVPVKLIGIGESLEDLRPFDADDFARAILEA"
     gene            complement(28847..>30172)
                     /locus_tag="VF752_01885"
     CDS             complement(28847..>30172)
                     /locus_tag="VF752_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8854322.1"
                     /translation="EDAQEAVSRLERAIARRREEPTGGPADVRHAELAAELRAERRLL
                     EQARREQAERTARIERLRARIERDDSLAQAAERAAAALSAAAEAVAARRDQLTAELEA
                     DHEAGEATAAELRVCALQEAGLQEKLREASQEVTAAEVRAQQARDFGAEAGSELERLA
                     ERLGLDPAPAAEPLAEERQAELESRVERLARRREQLGPVNPLAKQEYEEAVAHVEELE
                     EQRRDLEAALAELDGLIKETDKRIRESFEETFEAAAKNFEEVVQHLFPGGRGRLRLVH
                     PDGPRPVIGGEDGKPAVDSAEEGGAEPGDVVAAEPDPDEAPAVDEPGVEVEVTPAGKS
                     MKRLSLLSGGEKSLVALAFLFAVFLARPCPFYILDEVEAALDDLNIDRFLQLVRQFSN
                     RAQFIVVTHQKRTMDAADCLYGVSMGGDGVSKVVSRKLAAIPAGDEAAA"
BASE COUNT         4252 a        10815 c        10410 g         4695 t
ORIGIN      
        1 tcgcctcgag cgtgccgccg aactgctcgc gcaccggccc tggcgcttct gcgcagcctt
       61 cggcctgctc gccgggttgg ttgtcgcggc gggtcacgcg ataggcgacg gtgccctccc
      121 gctcagggca ggcgcgctcg ccgcgggcgc ggcgctcacc ggcatcgagc tgctcgctgt
      181 cgccgtcggc ttcgccacgc tggggcgctg gctgcgcctc tggagcccta gcgaccatcg
      241 ggcgtgagcg ccgccgcgag atacgagtag tccgccggct cgccgtcgtc ggtcacgagc
      301 tgggcgcatc cggcgcgtag gtcctcgcag tcggctcgcc ctccctggct cagcccgatg
      361 tcgagccgcg agcggtagta cggccggcgc gagggccggc agcgcggccg ccagcccgag
      421 cgtgcccctg aatcgtgtga ccaacgtccc tcctcgtgaa cgtcccctgc ggtctgagcg
      481 cgcgagcgcc gctggggata accccacacg ccattcgcct gtcagcggga ttgcagcaag
      541 ggcgcgcgat ctcgatcatg aagcccgtga gcaccgacac agccacaacc accgtcctcc
      601 ggccaacagg cggcggcggc gtgcgcgccg tgctgcgcgc tccgttcgag gcgcgcacct
      661 ggcgggcgag cgcctacctg cttctgagct tcccgatcgg ggtcgccgcg ttcgtcgtgc
      721 tcgtgacggg gttcagcctc gcgttcggct tgctgatcac gctgatcggc atcccgatcc
      781 tgctcggaac cctcgcggtg gcgcgcggct tcgcgatggt ggagcgccgc cgggctgctg
      841 ttctcgaggg cacgccgatc ccggatccgt accggccgat tccggagggc cgcatcttcg
      901 ggcggctgtg ggcgtggctg aagcaggcga gcacctggaa agacgtcgcg tggctcttcc
      961 tgctcttccc gctcggcatc ttcgacttca cgctggtcgt gaccgtctgg gccgtcgcga
     1021 tcggcatgct gacgctgccc gcctggttct gggcggtgaa cagcgacccc gacagcctcg
     1081 acttcgggct cttccacgcg aacacgctgc cgaaggcgct gatcgtcgcc gcggtcgggc
     1141 tgctcgcgac gctgctcgtg ccctggatcg tgcgcggctg ggcgcacgtc gagctgctga
     1201 tgcagcgcgg cctgctcggc cccgggcgcg aggagctcga acagcgcgtg ggcgagctga
     1261 ccgccacgcg ggccggcgcc gtggaggcgc aggcgagcga gctcgagcgc atcgaacgga
     1321 acctgcacga cggcgcgcag gcacggctcg tggcgctcgc gatggacctc ggcatggccg
     1381 aggagaagct cgactccgat ccagaccacg ccaagcagct ggtgggcgag gcgcgctcgc
     1441 aggcgaaaca ggcgctcacc gagatgcgtg acctggtgcg cggcatgcag ccgcacatcc
     1501 tgcgcgaccg cgggctcgac gccgcgatac ccgcgctcgc cggcagcacg cggttacata
     1561 cgaccgtgcg cgtggatgtg ccgcggcgac tgccggccgg cgtggaggct gctgcctact
     1621 acgtcgtctc ggaatcgctc gcaaacgtgg cgaagcacag cggttcgcag ttagcggcga
     1681 tcgacgtggc gctcaacggc gactggctcg tggtgcaggt caccgaccag ggcagcgggg
     1741 gcgccgacca gaacggcggc ggactcgccg ggctggccaa gcgcgtgcgc gcactcgacg
     1801 gcaccttcga cgtcacgagc ccgcccggcg gcggcacacg cgtgagggcg gtgatcccgt
     1861 gcgcgtcgtg atcgccgagg atctcgcgct gcttcgtgac ggcctcacac gcatgctgcg
     1921 cgacaacgac atggaggtgg tggccgcagt cgaggacggc acctcgctcg tcgagtcgat
     1981 cgtggagcac aagccggacg tggccgtggt cgacgtccgc ctccctccca gctttaccga
     2041 cgagggcctg cgcgcggcgc tcgaggctcg ccagaaggtg ccgggcacgg ccgtcctcgt
     2101 gctctcgcag tacgtagagc agacctacgc cagcgagctg ctcgccgagg gcagcggcgg
     2161 catcggctac ctgctgaagg agcgagtggc cgacgtgcgc gacttcgtag acgcggtgcg
     2221 ccgggtcgcg gagggcggca ctgcgatgga tcccgaggcg atcgcccagc tcgtggccaa
     2281 gcgcggcgcc gacggcccgc tcgaggagct caccccgcgc gagcgcgagg tgctcggcct
     2341 gatggcggag ggtcgctcga acgccgccat cgccgaggag ctcgtggtca ccgagggcgc
     2401 gatcgagaag cacatctcga acatcttctg gaagctctcg ctgccaccgt cggacacgca
     2461 ccaccggcgc gtgctggcgg tgcttgcgta cctacgcggg tagcgcctgc tcggcgccgc
     2521 gtgaacgggc gccgagagcc ccgagcagcg cgccgatggc gatcgtgaac acgagcagca
     2581 gcaccggcgg atgagggagt gcctggatct cctcgccgtt gtcggcgagc tcgtgcgcga
     2641 tcagcagcgc tgagccgaac agcagcccgc cccacgtgcc gcggagagcg ccggtgcgcg
     2701 gcgtgacgtg ctcgcgcccc gccgcgaagc cgccgatcgc ggcaagcacg tttgcgacca
     2761 cgtaccaagt gcccgacacg ccgagcagaa gcccggtggc ggcgcccgag acgatcggga
     2821 gcacgccggc gttgacgacc ttgatcgccg cgggcagctc ggcaaagagc gggggtcgcc
     2881 agagccgctc cagcacgacg ctaacgggtg acctcgtagc gcagctcgac catgccggaa
     2941 tcgaacgcgg tcgtgccggt gagcgtgaga ggcggtcccg ggacgcgacg gtcgaacagc
     3001 ggcttacccg agccgagcac cacgggcacg acagtcacga tcacctcgtc caggagcccc
     3061 tcgtcggcga actgcgaggc gacgttgccg ccgccgatga cccacaagtt gccctcgccg
     3121 gccgccccgg ccatctcgtc gtagatctcc ggcaccttcg cgttcacgat ccgaacgtcc
     3181 acggcgttgc cctcgggcac gcgcagttcc ctcgtgctca gcacccagaa cggctttccg
     3241 gggtacggcc attcgtcgac gtggtcgagc acccactcgt acgtgacgga gccgctgatc
     3301 atcgcgccga cgttctcgaa gaagcgctca tacgagccgc ggcccccggg ctccgcgccc
     3361 tcgaccgcgg aactgccctc gtagcccgtg agccagtcga gcgtgtcgtc tgcttcggcg
     3421 atgtacccgt cgagcgagga ggcgcagtag tactgggtct tgctcacggc cgcggcaggc
     3481 tatccggcgc cgagcgcctc gtccagcgcc tcgttcacga gctcgtcggc gcgctcgttc
     3541 gactcgcgcc gcacggtccg cacgctccag ctctcgaact cgcgaagctg gttcatcgtc
     3601 tccacgaaca gcgggcgcag gccggcgtgc ttgaccttgt actgcccgcc gatctggcgc
     3661 gcgacgagct cggagtcgtt cacgacctcg acctcgttgg cgccgagctc gcgagccagc
     3721 tcgaggccga gcagcactgc gcggtactcg gccacgttgt tggtggcctc gccgatgtac
     3781 aggttgcgct cggcgagcac cgagccgtcg gtcgcggtcg cgacggccgc tacggccgcc
     3841 ggccccgggt tgccgcgcgc accgccgtcc acgtggacga cgaccttgtc gagccgctcc
     3901 tggacgatga cgctacgccg gcggctcgat cgacgggaag ctgccgtagg cgcggcgcct
     3961 gcggcggcga tccggcggct gcgggttggt cgggtcgccg ccctgcttgc gccggtcgat
     4021 gatgacctcg acattcgggt cgtcgcggta gtactccacg agacggtcgt agagctcgtc
     4081 cgcgagggcc tgtggcacga cgcagtagat cactccgcgg accctacacc gaccgccagc
     4141 cgtccatccg ccgtcatcgc ggcgttccag gcgcgcagct gctcgtcgat ctgatcgagg
     4201 tcctcgccct tctcgagcat cgtgaccggg tcgtccgcgt cgcccagatc cacgcgatag
     4261 cggtaccagg agagctccca ggcgaccacg atctccacct cgctcggctt ggtctcgagc
     4321 ggcgccgcgt agacccacgg gtcgccgagg ctccgagcga tgccgaggat ggtgcgcgga
     4381 tgctccgatc cgttgaagac ctccagcgcg cgctccacct tcacctgggc attggtgggc
     4441 acggcgcgga cgtggcgcgg atccttgggg cgccaggcac gcgtgcgctt gcggtccggc
     4501 ggcggctcgg gagcggcttc ggcggcgggt tgatgctcgt cgtcgtaggc gagcgcgggt
     4561 ggcggtgccc actccggctc cggatggccg ttgtcctcgc ggcgtcctcg cagacgcccc
     4621 agcaggccgc ggcgcggctc gcggcgcggg tgcgccgtcg gcatgtcgcc atgcgcggac
     4681 tcgcggatcc agccttcgtg ctgggcccgg ccgatgcaga ggtcgcacac cagccggcgc
     4741 tggcctccgg ggacgagata aggctccgcg cgctcgccct tcaggatcgt gcgcccgcag
     4801 acgtcgcacg tgaccacgtc gtgtgtgggt cgtagctctc taggcatagg caaccttcga
     4861 tcctagcgcc ctgtttcggg cgcattggcg cgcttcctcc ccggttgtcg ctagctacgc
     4921 cttgacggcc tccgcctcct gggacgccgc ctggatcacc ttctgggcca gatgctgcgg
     4981 cacttcctcg tagcgggcga ggtccatcgt gtaatcgccc tggccgccgg tgatcgcacg
     5041 caggtccggt gcgtagctga gcatctcggc catcggcacc tcggccttga cctcggtcac
     5101 cgaccccttg gggtccatgc cgagcggccg cccgcgccgg gagttgaggt cgccgatcac
     5161 gtcgccgacc gagtcgttcg ggaccgacaa ggtgacggtc atgatcggct cgagcaggac
     5221 cgcgccggcg ttcccagcgg cctccttgaa cgccatcgag cccgccacct tgaacgccat
     5281 ctccgacgag tccaccgagt ggtagctgcc gtcgtagagg cgcacccgca cgccgctcat
     5341 cgggtagccg gcgacgacgc cgtcgcgcat cgcctcggcg atccccttct ccacggccgg
     5401 gatgaagccg gaggggatca ccccgccctt gatcgcgttt acgaactcga agccctggcc
     5461 gtcgggcagc ggctcgatct cgatgtggca gtcgccgaac tgaccgcggc cgccggtctg
     5521 cttcttgtag cggccgtgcg ccttggcagc ggtcttgatc gtctcgcggt acggcacgcg
     5581 cggcggcttc aggttcacgt ccgcgccgaa gcgctcgtgc aggcggtcga cgatcacctc
     5641 cacgtgcatc tgcgagagcc cggcgaggat ctgctcgccg gtctgtgggt cgcggtgcac
     5701 gtcgatcgtc gggtcctcct cctgcaggcg gcgaagcgct gagaacacct tgtcctcgtc
     5761 gcccttcgac ttcggctcga gcgcgaacgc catcaccggc gccggcatgg cgggcagcgg
     5821 cagctcgatc ttcgcgtcgg agctcgacaa cacatcgccg gtgtgcgtgt ccttcagctt
     5881 ggccaccgcg ccgatgtcgc cgggtccgaa gtccgtcgcc tgttgcgatt ccttgccttg
     5941 aatctcgagc agctgaccga tgcgctcctt ctggcccgag cgcgcgttgt acacctgcga
     6001 gtcggcgttg atcacgccct ggtacacgcg gaacaggttg atccggcccg tgtagggatc
     6061 ggcgagggtc ttgaacacgt aggcgaccag gtcgccgtcc tcgctcggct cgagcgtgat
     6121 gtcgccggcc tgcaccggtc ccttcttgcc cggcgacggc agatcctcga cgaacgcgtc
     6181 gagcaggcgg ttcgtgccga ggttgtgcgt cgccacgccg cacgtgaccg ggaacagccg
     6241 cccagccgtc acgcctgcct tgagcgcctc gactgtctcc tcgtgggaga tctcctcgcc
     6301 ctcgaggtag cgctccatca gttcgtcgga gttctcggcc acctcgtcga ggagcttctc
     6361 gcggtactcc tgcgcctggt cctgaagctc ctcggggatc tcgatctcgc ggcagttctc
     6421 gcggtccgag ccctcgtact cgtaagcctt catatccacg aggtcgatca caccccgcac
     6481 ctcgtgctcc atgccgatgg ggatctcggt cgccaccacg tgtggcccga aggcctcttt
     6541 cagcgactcg agcgtgcgga agaagtcggc ccgctcgcgg tccagcatgt tcacgaacac
     6601 gacccgcgcc aggcccagct cccgcgcgcg atcccacagg cgcgccgtgc ccacctctgc
     6661 gcccatgacc gcgttgacca cgaacacagc tccctcgctc acacgaagcg ccgcgagcgc
     6721 gtcagccacg aagctcggct cgccgggggt gtcgatcagg ttgatcttgc ggtcctccca
     6781 gtggagcgag gagaggctcg atgtgatcga catctggcgc gatttctcgt ccgctgccga
     6841 gtcggacacc gtcgttccgt ctgccaccga gccaaggcgg ttgagcgcgc ccgcctcgaa
     6901 caggagcgcc tcgttgagtg aggtcttccc gctgccccgg tggccgacta gggccacgtt
     6961 gcggatcctt ttcgccgatt cgtgcggcat ggcacggctc ctctcctcga ctaagtggca
     7021 gacccaacat acctgcctgt caaccgaacg cacgttcgac ccctcgcctg cgcccctacg
     7081 aacgcttcgg ggtcgaacgt gaagcggagt tcgggctaga ggctccgaaa accgaggatg
     7141 gcaaggacgc agggccgaag gcgtgctcgg cacgccgagc gcccgaggac gcagccagcc
     7201 gaaggtttgc aggggcctca ggcgcccgag gacgcagcca gccgaaggtt tgcaggggcc
     7261 tcagccggcg gcgtcttcga gcatgtcagt ctgcaggcgc gacttgaggc ggagcaccgc
     7321 cttggtgtgc agctgcgaca cgcgcgactc ggtcacgccc agcacctcac cgatctctcg
     7381 gagggtgagg ttctcgtagt agtagagcgc gatcacgagc ttctcccgct ccggcaggcg
     7441 tgcgatcgcg tccgcgagac ggtccttcgt ctccgacgtg tccatcgcgt gggccgggtc
     7501 ggtggcctgc ggatcctgga tcgtgtcgag gagcgacacc tggtcgccgg acgcgtccga
     7561 cagcgtccag agctcgtcga gcgccaccac cgacgagttc gagatccgcg tgagcgacgc
     7621 ctggaagtcc tccaccgtgg tctcgagcgc ctccgccatc tcctggtcgg tcggcgctcg
     7681 gtgcagcttg tgctcgagcg cggcgttcac cttctcgatc tcgcgcgcct tcgcccgcac
     7741 cgagcgcggc acccagtcga gcgagcgcag ctcgtcgatg atcgagccct tgatgcgcgt
     7801 gatcgcgaag gtctcgaact tgatctcgcg cgccgggtcg aagcgctcga tggccgagat
     7861 caggccgagc aggccgtagg agatgaggtc ggactcctcg acgtgcgagg gcaggcccgt
     7921 ggacatccgc cccgccacgt acttcacgag cggcgagtag gcgagcacga gacgttccct
     7981 ggcggccttg tccgcatcgt ccttgtagcg gcgccaaagc tccctgagct cgatctcctt
     8041 gacgttcgtc tccaggatgt cccccgaaat tcgaaagctc aggctcgtgg gtgagcctga
     8101 gcgtaagccc gtttcaggcg tttcgactct acccgagtgt aagtctgggt tgtgctgatc
     8161 gtcgcatggc cgagcagctc ttggatggat ctcaggtcag cccctccctc gagcaggtga
     8221 gtcgcgaagg agtgcctgag cgtgtgcggc gagacgccgc cggcgagtgc ggcacggcga
     8281 gtccacagcc cgagtctcct gcgcacgtcg gacgtagaga gccgctgacc ggtcttcgag
     8341 agaaacagcg cgggctccac gcgagcgccc tcgacggcca gcgcgggtcg gccgcggtcg
     8401 aggtacgact cgagcgcctt ccacgcgagc tcccccgccg ggacgatccg cgtacggccg
     8461 cccttgccct gcacgcgcag ctcctcgcca tcggcgtcgg ggtctgtgag gtcgaggttc
     8521 acgatctcct cggcacgcag cccggctgca taggcgagct cgaacatggc gcggtcgcgg
     8581 agctcgagcg gcgtcgcggc cgggatgcgc tcgagcagct gcgccacctc gccgggtcgc
     8641 aggacgcgcg gcaggtaggc gtcgcgctta gggctcgcga cgaggtcggc cggactcgcc
     8701 tcgagctcgc cgcgctcgac catctgcctg tagaagctgc gcacggccgc gagcttccgc
     8761 gccaccgtgg actttgaggc gccgcgctcc gagagcaccg cggcatagcg ccgcagctcg
     8821 cgcgggccga tgcccttcgg ctcgaggccg ctgcgcgagg cccactcggc gagctgctcg
     8881 aggtccgtcc cgtacgcgcg gcgcgtcttc tcggccatgc cgtgggcgcg cagcgcgcgg
     8941 tcgaaccctc gcagcgcgtc agcccaggac ggcggcgggg agatctcatc catgcggggg
     9001 acgttttcga cgccatagac gggacgcctc ccgtgcggtc aggagatgta gacgggggcg
     9061 cctacgggca cgtgcgggta gagctgctcg acgtcgggaa tcgacatgcg gatgcagccg
     9121 tgcgacgcgg ccgtgcccag cgaagcgatg ttgtcggtgc cgtggattcc tgcgccgttg
     9181 aagatcccga gccagcgcgc cttgagcggg ttgttcgggg cgccacccgg aatcaccttg
     9241 ccggcgagct tgcctgccca ggacgaatgc ggcacgttcc agggcgggtt tatctgcttg
     9301 ttctggatgt ggtagagccc ggcgggcgtc tcgagcccca ccatgcccac cgcaatccgg
     9361 tacgtgtgat tgagcgagag gtgcctgtag aacttgagct gcttggcgtt gcggtcgacg
     9421 atgatgatcg ccggatagcg ctgggccagc tggtcggtgg tcaccttcgg cttcgtgatc
     9481 ttcgaatggg cgcggacgac gcggtcgccg tcgggcatca cgagcgcggc ctcgatgttc
     9541 cgcgtcaact cgacaccccg gatggcgttt ccctggtgcg agggcaccgg ctggaacgcg
     9601 cccgaggtga aggacacggt agcgtcgacg gccgggcggt cgagctgcga cttgacgcgc
     9661 cggacgagcc gctcgacggc tgcgtgggag tagctgatct ccggcgtcag ctgcgcgtgc
     9721 acctcgccgc cggtcagcga gcgccagctg cgcgtgacga tgttgccctg ccggctgcgg
     9781 cgcatggcct cgtccacgat cgcgtcgaca ttggtcctga ggtgcgcgcg ccgcgccgac
     9841 aggcggaagc gctcacgccc gtacttgacc acgagcgggc gggaaagcgg cgcggcgagc
     9901 tgctggtcga gagccgtgcg ggcctggctg cgggtcatcc cgccgaggtc gatgccgctc
     9961 accgatatgc ccttcgcgac cttgtcggtg cgtgtcgagt cataggcgta gacggccacc
    10021 gctccgccga tcatcacgac gagagcgact gcaagagcga tgaaggagcg gtgcctcatt
    10081 gtttgaatcg gtgcaaggta gccggtatgc cgcgctttgg cgagtgggtc cttcccctgc
    10141 aacgccgctt gcgaaaaacg cgtgctattg cgggcattcc cggcgccacg cctgtgcaat
    10201 cgctcttgtc aggggtgtga cgaggaggcg gcgcggcgcg cgaggatgga ctcgatcgcg
    10261 gcaatcacag gttggcgcgc ctgtgctctc cgctcatatt cgcgcagcgc ctcgaggtca
    10321 gccagctcga gagaaccgag cagtgagatg acctcctcgg cctcgagatc gtcgtagtgc
    10381 gcgaccggcg gggcgggcgg ctcggccggt ttcgccttcg gcgtcctcga cctgcgcacc
    10441 gctcgcgggg gtgcctcgct ctggccgttt ccctcgtcgt actcctcaag gaacttgcgg
    10501 agctggaacg cctgccgcgg gtcgagcgtg ccctcgcggc ggtaggcggg gttcttcacg
    10561 tgcgggctcc agcgcaggaa atcctcgacc tcacgctcgt agcggtatgg ctcctggacg
    10621 ccgcgcagga tcgggctgag ccgcaggtat tcggcgaact tcgcgacggt ctcgcgcgtg
    10681 gtgcagcggt actcgaaacg ggtggccttc agcttgcggt tgtcgagccc gcgcccgaag
    10741 cgcagctggc tcagcatctc cggagggacg cgcacgccca agcggctgag cacgtttgcg
    10801 gcgagccccg tcccccacgg cggcaggatc ggcgcatagg gcctgccgag caggcctgcg
    10861 acctcgctga gcgcgagcac gccgtctgcg gccacgttga agatcccgcg cagctcgaat
    10921 tcgacgccgt gcttcagtgc ggcgaccacg tcgtcctcgt ggacgaactg gtagcgcgga
    10981 tcgaagccga gaatcatcgg gaccgccggg agcgagaaga gcttcgtgtg cgaggtctcg
    11041 acgtccgggc cgagcacgtt cgcgaagcgc atgaccgtga cggacacgtc ctcgttcttg
    11101 tcggcgaagt cggcgacggc ggcctccgcc tcgacgatgt cgcgctcgat cggcgtggtc
    11161 ggcgggtgcg ggcggcgcat ggcctcggtg aagaaggccg ggtcgtcctg cgccgtgccg
    11221 tagtagtgcg ccgagctctt gaagacgaac ttcctcaccg tgctgttcgg gcccgagcag
    11281 gcggcgagga tgttcatcgt cccgatcacg ttgttctcgt gcgcggcgct cggcttgagc
    11341 acggaggagt ccggcacgag ccgcgtgtcg atcaccgtgt cgatctcggc cgcctcgacg
    11401 atccggcgca gcagcgcgtg ctgggtgccg accttcacga actcggtgcg gtcgagctcg
    11461 cgcgtcgggt cgcgcgagtc gacccccacg atcgcctcga tctcggaatc ccgctcgagc
    11521 gcctgcgcga ggcggccgcc ccagtaggtc gagatgccgg tgacgagtac gcgcttcgaa
    11581 ttcatccgaa ccacaccgac tcgcgctgct cgaccatttc gaacacgttc tcctggatgc
    11641 gcgcccggat gtcgtgggcg tagctctgca caaggccgcg gtcgtcggcc atgtcctcgt
    11701 ccgggaactc gatgggctcg aggaagcgga tgcggaactt ggccggtagg tagccgagca
    11761 tgccgagcgg cccgaggtgc gggaacgtgg gcgtgatcgg gaagtagatg agccccgtga
    11821 gacggcgcag caggccgacc tgcgcgaaga tcggcgcggc ctcctccgcc cccactacgc
    11881 acaccggcac gatcggcgcc cgcgcgcgca tggccgcctc cacgaagccg ccgcgcccga
    11941 agcggcgcag cttgtagcgg tccttgtaga gcttctccgt gcccttgcgc ccctcgggga
    12001 acacgagcac gagctgctcc tcgtcgtaga gcaggcggtg cacgttggcc gggtgcgccg
    12061 ccacgcagcc gatcttcgga atcagcatcg agaagccggg atagcccttg aagaagtgct
    12121 cgaccgtcac gttcagtggc cgcggatggg gatgctcctc cttgatcgcc ttgccgatca
    12181 tggcggcgtc tggcgggagc gcgccggcgt ggttcgacac gagcaacccg cctcccgtgt
    12241 cgggcacgtt ctcgatgccc tcgacctcga cgcggaacca gtagcggtag aagaactcga
    12301 gcagatagcg gtcgaagacg ccctcgacgc gctcggagcg gccccagtcg ttcagccggc
    12361 gctctggctc aatggcgggc aagtagcggc gcagatcggc gtccgcgggc gcgggcaggc
    12421 cctcgccgct ggctggccgg gcgacgagct cctcagacgg gcgcaggctc gttcgcgtgc
    12481 tatcggccat gtggcccctc tgccatgcga gcgattctcg caggatccgt cgcgacctac
    12541 gctgtgaccg gctgccgcgc gtactacgat ccgccctcgg atgcgctcca aggacgaggt
    12601 gcggcgcgag gtgtggcggg cgatggaccg ggagggggta tcgcgcttcc cgggcgccga
    12661 gggacggatt cccaacttcg cgggggcaaa ggccgcgtcc gagcgtctag cgcgccaccc
    12721 cgactgggtg cgtgccgaga cgatcaaggc gaacccggac gcgcctcaga cccactcccg
    12781 gcgcttggcg ctcgcacagg gcaaatgcct cgtgatggcg gtccctcgac tacgcgacga
    12841 gcacccgttc cggctgctcg acccgcgccg gctctcggag gagcagcagc gcgaggcggc
    12901 cacgatcaag ggcgcgctgc ggcacgggaa ggtgatcgac ctcgagcagg tgccgccgct
    12961 cgacttcatc ctcaccggct cggtggcggt gaacctgaag ggcgtgcgga tcgggaaggg
    13021 cggcggcttc tcggatctcg agtacgggct gctcgcggag tccggcaagg tcgatcggcg
    13081 caccgtcgtc gcgaccaccg tccacccgat ccagatcgtg cgcgagaacc tgatggtcac
    13141 cgggcacgac atcccggtgg acatcgtctc gacgccgcgg gcggtgatcg aggtcgagcg
    13201 ggcccacgag cggcccaccg ggatcctctg ggatcacctc cagccgccgc agatccacga
    13261 gatcccggtg ctcgagcgga tgggctacgc gggctagctc ggcgccggct cttcggcgtc
    13321 gatgctcgag agcagcggcg cgactcggtc gcgaagcgcc cgccagtcct cgctcacgat
    13381 cacgcgctcg tcgtccgcga ggtgcgcgtt ccaggggtgg atgagcgtgg ccgggacgat
    13441 ccccacctcg agggcgcgct ggatgttcac cgggctgtcg tccacgagca cggcgatctc
    13501 gagctccacg cagcggctga ccttgtcgta gctgcagtgc aggtcgtcgt agggcaggcc
    13561 gatctcgtcg agccagccct cggtggcctc gcgcgcgctc tcggcgcggt ggctcgtcac
    13621 gtggatgaag tggccggcat cgtgccatgc cctcacggtc tcgacggcgt gcgggtaggg
    13681 cttcgccccc atgatgttct cgaccgagtg cgtctcctcc acgcacgcga tcagctggtc
    13741 gcgctcgagc agcgcgatgc cccagtcggt ctgctcctcg tacggcagct cgactccgaa
    13801 tcgccgctgc actatggcgt cgaagacgtc ccagtagtga tgaagcgtgc tgtcgatgtc
    13861 cagcgcgatc tttgccacgg gccggtacgg tactcggcca tgccgatctt cccgtcgaag
    13921 gctcggcccg cgagcctccg ggctgcgcgg cgcgccgaca gcgactacgg ggtgggcgcc
    13981 tccccgaact ggcgcgacat cgactggcgc gagcacctgc gctcgaccga gatcgacggc
    14041 aggcgggtga actacgtcga catcggctcg ggcgacgggc ctcccgtcgt gttcatccac
    14101 gggctgggcg gcgcctggca gaactggctc gagaacctgc cgcgcgtggc gcaggagcgc
    14161 cgcgcgatcg cggtcgacct accgggccac ggccagtcgg ggatgccggc cgagaaggtc
    14221 tccatctctg gctacggccg ctgcgtcgat gcgctcaccg agcagctcgg cttctccgag
    14281 gtcgcactcg tgggcaactc gatgggcggc ttcatcgcgg cggagatggg gatccagttc
    14341 cccgagcgag tggcacagat cgtgctggtt gcaccggccg gcatcaccac cacggagctg
    14401 cgccgtgcgc ccgcgctcac gtgggcgcgc accgcggcgg cgctcggagc ctgggccagc
    14461 acccgctcgg agatattcgt gaagcgcccg gcccttcgcc atgtagcgct ccagatcgtc
    14521 acccgtcacc ccagcaggat cgcgcccgac atggcgtggg agatgatcgc ccactccggc
    14581 aaggacgggt tcgtcgacgc gacgcgcgca ctgctcgagt acgacttccg ggaccggcta
    14641 cccgagatcc gctgccccac gctgatcgtc tggggcgacc gcgacgcgct gctcgacgtg
    14701 ggcgacgccg acgagtacgc gcgcctgatc ccggactccc gcaaggtgtt gatgcaggac
    14761 accggccaca tggcgatgct cgagcggccg gtggccttca acgactgcct actcgagttc
    14821 ctccacggcc aaatcgacac gagcggcgac tcggacgccg agcgcgccgc cgcaggcgag
    14881 gaagcggccg cctagcgctc aggtcgtgac ggcgccgcgc gacgcggagc cgacgtgacg
    14941 cgcgtacttc ccgagcacgc cgccctcgta agcggatggc ttcggctcgt aggcggcgac
    15001 gcggcggtcg atctcgtcct ggtcgagctc gacgtcgagc ctgcgatccg gcacgtcgaa
    15061 cacgacgatg tcgccgtcct cgacggccgc gatcggccca ccgcggggag cctcgggcgc
    15121 cacgtgcccg gccatgaagc cgtgcgtcgc gcccgagaag cggccgtcgg ttaggagcgc
    15181 caccgattct ccgagcccct cccctacgag cgccgcggtg accgccagca tctcgcgcat
    15241 gccggggccg ccagaggggc cctcgttgcg gatcacgacc acgtcgcccg gctggatctg
    15301 ctgcgccttc actgccgcga aggcctcctc ctcgccctcg aacacgcgcg ccggtccgcg
    15361 gtgttcgcgg cgatcgtggc ccgccagctt gaccacgcaa ccctcgggcg cgaggttgcc
    15421 gcgcaggatc gcgaatccgc cggtcggctg gatcgggttc gacagcggcc ggatgacctc
    15481 ctgaccctcc gtctcgttcg ccgcgtcggc ctcctcgccg atggtcttgc cggagaccgt
    15541 gggcgcgtcc tcctgcagca gcccggcctc gctcagccgg ttggccacga cccgcatgcc
    15601 gcccgcggcg tgcatgtccg gcgcggtgaa gcgcccgccc ggcttgaggt ccgccagcag
    15661 aggggtcttc cacgcgatcc tgtcgaagtc gtcgatgtcg agcggcacgc cggcctcgtg
    15721 cgcgactgcc aggagatgga gcacggcgtt cgtggagcca ccggtggctg ccacggaggc
    15781 gatcgcgttc tcgagcgatt cccgcgtgat cagcgagcgc gcggtgacgt ctcgctcgag
    15841 caggtcgagg acgagctgac ctgcccgctc cgcgacatcg cccttgtgct cgtcgacagc
    15901 cgggacgctg ttcgagccca tcggcgagat gcccatcatc tcgaacactc ccgccatggt
    15961 gttcgcggtg tactggccgc cgcaggcccc cgggccgggc gaggcgacgt cctcgagctc
    16021 ctgcagctcc tcgtcgctca tgctgccggc ggcgtgcgcg ccgaccgcct cgaagacgtc
    16081 ctggatcgtg acgtcctgac cgcggaagca gccgggcagg atcgagccgc cgtacagcat
    16141 caggctcggg atgtcgagcc gcgccagcgc catcaccgtg ccggggatcg tcttgtcgca
    16201 gcccgagatc gcgatcacgg cgtcgaacat gtggccgcgg gcaacgagct cgatcgagtc
    16261 ggcaatcacc tcgcggctca cgagcgacgt cttcatgccc tcggtgccca tcgtgatccc
    16321 gtccgagatc gcgacggtgt tgtattccat cggcgtcccg ccggcctcgc gcaccccggc
    16381 cttcacgcgc tccgagatcc ggcgcaggtg gaagttgcag ggcatcgtct cggtccaggt
    16441 gttcgcgacc cccacgatcg gcttcttgag cgcgtcgtcg tcgaagccga tgcccttcag
    16501 gtatgcgcgc gcagcggccc ggtccgggcc ttcgaggatc gcccggctcc gacgtcggat
    16561 gtcgacgctc atcggcggcg cgcctcgtac gagccggagc tcctgcgctg gcgctcccat
    16621 tcggccaggc gccagaggtc gcggcgtacc cgcccagggt cgatctgccg cccgggcagg
    16681 tgcatgttcg cgttgcggat gaggttgcgc tcgtcaccgg tgagcgccat ccacttcgcc
    16741 agcacgtcgt ctgagtccgg cagccgcgag tgcgagatcg cccgcgtgag gtccgggaac
    16801 tcgacgcagt actcgctcag cagccggcgg gtcttcggca cgaaggccac gatcggcttg
    16861 tgcgggtaga tcaggtaggc gtacggcgcc tccggctcgg gcgcgtcggc cgcttcgcgc
    16921 gggcgccggg gggcgctgcc gtcggccagc ttgccgttga ccttgatgaa gccgaggtcg
    16981 cggtacatcg accactcctc gtcgttcacg cacgagcgca gaagttcgcg cgcgcgctgc
    17041 tcggcgcggc gctcgcggcc cgggtcgtgc agcatcactc gctcgacgcg gcgcgtgcgg
    17101 aacagcggcc cgtgctccct gcgccagctc gaccaggcct gcacgagtac cggcagcgcc
    17161 agaacgacgg caaccagggt cagggccagc agcgcgagca acatcgtcga gccgtcggcg
    17221 cctcagccgc ccgaggcacg cgggaagaag atcacgcgtt cggcgtcggt cgggatgtcg
    17281 gcgtacctct gaaccatctg ggcctcgcga cggccgctgt cctccccgac cgtgggcacg
    17341 gcggcccaca tcccctcgtc gagctggcgc cggaactcct cgaccagccg ctcctcgtcc
    17401 tcgcgcaccc gcgggtcgcc ctcggtcaac aggacctctc caaaaccggg cttcatacgc
    17461 aggaacttga tggctgtact cctgtgctcg tcgaacttga cgtcgcgatc cggccttgcc
    17521 ggcggctgtc ccgattatgc cagaggccgt tatccatgcg ggaaagccct ggaacggccc
    17581 atttctcagt cgccaccgcg gcgagccagc ccgagcacgt cgaccggacc ggccccggca
    17641 ccgatctcgt cgagcccgtc gcgcaccgcg gctgacgcga cgcgcttggc ctcccgcgcc
    17701 gcctccagtg gcgtccggcc gagtgcgagc tgggccgcca gcgtcgacga gtgcgtgcag
    17761 ccggacccgt gtgcggcacc actgtcgtag cgctcgccgg cgatctcggc cagcgtcgcg
    17821 ccgtcgaaga agacgtccac ggcctggtcg cggtggccgc ccgtgacgac gacgaactcg
    17881 ggcccgagcg cctgtagcgc ccgcgcgaga tcctcgggcg cgccgccggg agcagcggcg
    17941 agcagccgcg cctcgggcag gttcggcgtg atcgcggtaa cccgtggcac gagcagtgcg
    18001 cggagggcgt cgcgagcgcc cggctcgagc agttccgcac cgctctcggc gaccatcacc
    18061 gggtcgagta cggtcggcgc ctgcccgacc aagtcgagcg cccgagccac ggcctcgatc
    18121 gtctcggtgt tcccgagcat cccgatcttg acggcatcca cgccgatgtc ctcggcgacc
    18181 gccctcacct ggtcgacgat cgcctctggc gggagcggat agatcccggt cacggccacc
    18241 gtgttctgcg ctgtgatcgc cgtgatcgcg ctcatcccgt acacgcccgc gtgggcgaac
    18301 gccttgaggt cggcctggat gcccgccccg ccgccggaat ccgagcctgc gatcgtgagt
    18361 actcgcggta ttcgccgcgc gccgctctgc atggcgcccg aggatatgag gcttcaccgc
    18421 gcgggcgacg gctggtaggc gccgcggccg tccgggacga tcaagccggc catctcgagc
    18481 cgagccagcg ccgcgcgaac gcggccggcg cccaccgcgt gctcgcgagc gatggcgccc
    18541 gcgtgctggc cctcctcgac cgcgtcgagc accgtgcggt cgaagtccga gagctgctcg
    18601 aggacgtagg cccgccccga cgggtcccag ccctccggcc gcgcttctcc gtcgtagacg
    18661 tcatcgacga gatccgcgac gccgcggatg acgctcgcgg ctccgtcccg gagcagcttc
    18721 agacagccgt cggcgaaggg cgaggtggcc ggcccgggca ccacggcgag gccgcggttc
    18781 agttgcgtcg cgaagtcggc ggtaatcagc gacccggatc ggtccctcgc ctcgaccacc
    18841 aatgtcacgc gcccgagcgc cgccatgatc cggttgcggg ccgggaagct ccagcgaaac
    18901 ggcctgacgc cgggcggcag ctcggacacg accgcgcctc gctcgcgtac ctgctcgtag
    18961 aggcgcacgt gattgcgcgg gtacgggatg tcggcacctc ccgcgagcac cgcgagcgga
    19021 gcgccggcgg cgtcgagcgc tccgcggtgt gccgcgccgt cgatgccgag cgcgaggccg
    19081 ctcacgactg tgattcccgc tgtggtcgcg ccacgcccga gttcatgcgc catcgcgaca
    19141 cctgccgacg aggcgcgtct cgcaccgacg atcgtgacgg cgggtagctc cgtgatctgc
    19201 gcgagccggg ccgcggagcc cgtgtggtac agcaccgccg gcgggtcgta gagatcgccg
    19261 agccgctccg ggtagagcgg gtcgtgagcg cagagctcgc cgatgccggc acgctcggcc
    19321 atgttgcggg cgacgtccgc gtcgaagtcc tcgatgaagc gctcggccgc gtccttgcgt
    19381 ccgcccgcca ccgcctcgat gaactccagc tcgggcagcc cgagaatggc cgacggccga
    19441 gggcggggct cggcgaggac cgccgccacg cgcgccgcga ggaacgcgat caggtggctc
    19501 cgccgcagac aggcgtcgca ggcgctcatg ccgcgaccgc catcgacggc ggcaggatgc
    19561 ggtaggcaag cgcctcgtcc acgtcctcgt cgcagatcgc gtctcgaccg tcgaggtcgg
    19621 cgatggtccg tgcgacgcgg agcacgcgat cgtgcccgcg ctcggagatc gcgcccgcca
    19681 ccaccgcttc cgcaagccgt cgggccgcag ccggatcgag cgcagcgtga cggcgggtct
    19741 cgcggacgtc catgtcggcg ttgcacatcg cagcgcttcc cgacaggcgt tgcgtctgcc
    19801 actcacgggc ggccgcgaca cgcgcggcga ccacctcgga tgactcgcct ccctcacccg
    19861 gcgcgtcgga gggcggctgg acctgacaga ccaggtccag ccggtcgagt agcggacccg
    19921 acagccggcg ctggtagcgg cgtacgtcgg tctcggtgca ctcgcacttc gcgagcgagc
    19981 gcccgcacgg acagggattc gaggccgcca cgaacatcac agatgccgga aaagtgagcg
    20041 actgctggcc gcggacgatc tccacgcgct tgtcctcgag cggctgccgc aacgcctcga
    20101 tcgacgggcg cgagaactcc gcgagttcgt cacagaacag gacgccgcgg tgcgcgagcg
    20161 tgacttcgcc ggggcgcggc gtcgagccgc cgccgatcag gcccgaggcc gacaccgtgt
    20221 ggtgcggtgc gcggaacggc cggcgcctcg cgagtgtgcc gtcgctcttc ccggcgacgc
    20281 tgtggattcg cgtgatctcg agcgcctcct cgaggctcgg cgggggcagg atcccgggca
    20341 agcgccgtgc gagcatcgtc ttgcccgacc ccggcggccc gaacatgagc aggttgtgtc
    20401 ccccggccgc cgctagctcg agcgctcgac gcgcgtcctc ctgaccgcgc acctccgcca
    20461 ggtccgggcc gtacgagcga tcgacctgcg ccggcaccgc cggcaccgcc ggcgagactc
    20521 cgagctcgcc cgccagcagc gcgccgatgt cgcgcagacg gtgaagcccg tagacctcga
    20581 tcccctcgat cacggcggct tcggcggcgt tctcggtcgg gaccacgatc cgtctgcgcc
    20641 cgctgcgcct agcgccggcc gccatagcca cgatcccgcg caccggtcgc agatagccct
    20701 cgagcgccag ttcgccgcaa aacgcccact cgtcgaggtc cacgcccggc aattgaccgc
    20761 tcgagccgag cagtgcaacc gcaagcgcca ggtcgaatcc cggcccggcc ttgcggatgt
    20821 ccgcgggcgc gaggttcgcg gtcaggcgtc gcatcggaaa ctcgaggccc gaattcagga
    20881 ccgcggccct cacgcgctcg cgcgcctcgc gcacggccgc gtcaggcagg ccgacgatcg
    20941 tgaagacggg cagcccgctt cgcacgtcca cctcgacggt cacctcgcgt ccctcgacgc
    21001 cctcgatcgc gaatgtcgca actctcgaaa ccacggcggc caagctaggc cgtgccgtgc
    21061 gactggtgtg ttacggacgc aacagttcgc cgaactccgc gtttttgcgg ttctgtaacg
    21121 ctcgtaaccc acgcgtcgaa cggggctcct taggttcgcc gccatggcat ctgacccccg
    21181 gaggcgactc ggagcgatcg gcgaggacct cgcggcgcgc catctcgagt gcgcgggcta
    21241 tgaggtcctg gagcgcaatg cgcggacgcg gaccggcgag atcgacatcg tggccgcgcg
    21301 cgcgcaggtg cttgtgttct gcgaggtcaa gacgcgggtc acgcgctcga cgggcggacc
    21361 aacgggcccg ttcgcggcga tcggcccgcg caagcgccaa caggtgcgcc tgctcgcgcg
    21421 cgagtggctg gccggcgcga gcggccgccc tcactacccc gatctgcgct tcgacgcgat
    21481 cgcggtcgcc ctcacggcaa gcggcgagct cgtggcgctc gaccacctcg aggatgcgtt
    21541 ctgatgttcc tacagagcga gctgctggta cgcgatcgag gcgaaggaca tgcgatgcag
    21601 cgggcagatg ccattcgcgg cgatcgcggc gcggtgttcg ggcgtggagt agccgacgtt
    21661 cgagtcgaac tcccagtcgg gatggcgctc gtgcgcgcgg cgcatgtagc gatcgcgagt
    21721 aaccttggcg agcaccgacg cggccgcgat ggcggcgcta cgcgagtccc ctccgatcac
    21781 cgcgcgctgc tcgtagccga agtccggcag cttgaacccg tcgacgaggc agatcgagcc
    21841 gggacaggcg acccgcatca gcgccgcgcg aagggccgcg atgttcgtca catgcaggcc
    21901 gcgggcgtcg atgccgcgca cgcagcggga gacgatcgag accttggcag cggcacgcat
    21961 cacgatcggg tagagctcct cgcggccctc ctcgtcgtgc tgcttggaat cgttgaggcg
    22021 cgacagcgcg cggcgatcgg agagcgtcag ccgctcgagg tcgaacagga ccgccgcggt
    22081 gaccagtggc cccgcgaggg aaccgcgccc ggcctcgtcg gcgccggcca cgaaacgccg
    22141 ctccagcgaa cggtcgaacg cgaacaggcg gcggcccgtg tgtgacggct tcttagagcg
    22201 tcccgatgcg tttcgggggc cagtaggtga agaaggcctc tccgatgatc cacttcttgg
    22261 ggatgggtcc ccactcgcgg ctatctgcgc tctgcccacg gttgtcgccc atcataaaaa
    22321 agtgccctgg cggaatggtg atggggttcg tctcattgca gatctcgcag ttggcgtcgg
    22381 ggcggatgaa gggctccttc tggagcttgc cgttgatgta gacgcgcccg gccttgacga
    22441 agagcttgtc gccgggcacc cctacgacgc gcttgatgaa gttcgtgtcc gagtgctgcg
    22501 ccgtcggcgt gtcgcaggcc gtgtttgccg gcatcgcctt gccgcagctg tcggtgtccg
    22561 cgcccttcgg cggcttgaac accaggatgt ccccgcggtg cggatccgag aagtgaagcg
    22621 tcactcggtc gacgagcacg cgctgcccga cggcaagcgt cggcaccatc gactcgctag
    22681 ggatccggaa aggcttgacg atgaaggcct ggatcagtag cgcgagcccg agggccacgg
    22741 ccacgatcgt gaccagctcg acgagcgagc cggtcttcga gtccgacttg cgcgacccgg
    22801 gcacgcgtgc aggccggctc accgcaccat caggacttct cgccgccctc gctcgcctca
    22861 ctggcgacgg gctcgtcggc gggcgcgtct tcgggcgcgc cctcctcgcc gtcggcctgc
    22921 tcggcagcgg ccggctcgtc ttcagccggc gactcgacgg ccggggcgtc ctcagcgggc
    22981 gcctcctcca ccgcggcctc gccggccgga acctcctcga cggtctcctc gccgaccgcg
    23041 gcctcagccg cgccggcctc cccagcgtcc tccgtctcgg gcgcgaggtc ggccggcggc
    23101 gcggcctcct gtgtcccgag ctcgtccctc gccacggcca gctccgggcc ggtgtactgc
    23161 cgctcgcgga cgcgcgcgcg gcggccgacg cggccacgga ggtagtagag cttcgcgcgg
    23221 cgcacgtcgc cgcggctcgc cacctcgatc cgctcgatct tcggcgaatg gacggggaac
    23281 gtgcgctcca cgccaacgcc gaaagactgc ttgcggacgg tgaatgtctc gcgcacgccc
    23341 ttgccctgcc gcctgatcac gacgccctcg aacacctgcg tccggcggcg gttcccctcg
    23401 accacctgga agtggacctt gaggcgatcg ccggcctgga acgcgggcac gcgcttcagc
    23461 tgggctcgct cgagactctc gattactcgg ctcatgacaa aagaatggcg tcaggagggg
    23521 gctcgtccct cacccgacgc gcgtcgcgca atggtagcga agggtcgcga gaccgacctg
    23581 gccgtcttgc gacgatctgc gggcggagat tagcggccgc cgcgacggcg actctgctcg
    23641 agccgccact cacggacacg cgcgtgattt cccgacacga gcacctccgg caccgcgtgc
    23701 ccatgccact ccgcaggccg cgtgtagtgc gggtattccg ggccgccctc gagcgccggg
    23761 ctgaacgact cctccaccgc gctgtcctcg tgcccgagcg cgccgggcag tttgcggatc
    23821 accgcgtcgc agatcaccat cgccgcgagc tcgccgcccg cgagcacgta cggcccgatc
    23881 gagagcacgt cggaagcgag gtggtcgtgc acgcgctggt cgaagccctc gtaccgaccg
    23941 cagaggaccg tgagctcggg ttctgcggcg agctcggtcg cgagctcgtc gtcgaactgg
    24001 cggccggatg cggcgagagc gatcacccgc cgctccttgg gcagttccag cgggtcgtga
    24061 ccgtaacgcg cccggagcgc ggcctcgacc acgtccacgc gtatcaccat gccagccccg
    24121 ccgccgtagg gcgtgtcatc gacctgtccg gcgcccagcg gcgtggtcgc ccgcatgtcg
    24181 atcgcctcaa ggcgatgacc gagcgcctcc gcgtttcgta cgtgacgctg ctcgcggaac
    24241 cagtcgaacc acccggggaa gagcgtgaac acgtcgatgt tcacgcgccg ccgccctcct
    24301 gttcctggtc gctcagcgcg aggaagtcgt ggtcgacctc gatcacgcgc cgctcgagat
    24361 cgacctcgcg aaccgcgtcc gaaaccagcg gcaccagcac gccgcggtcg cccacctcga
    24421 gcaggtcgca ggacggaccg gcgaggacgg cgaccacctc gccaatcccc ggaaccttgc
    24481 agccgaggag atcctccacc agccattcgt cctcggcgag cggcagctcg gactcggcga
    24541 cctcgatcgg ctcgccgctg agcgccgctg cggcatcgcg gtcgtccacg cccgacaccc
    24601 gcagcagcgc gcgctcggca tcgccgtcgc gtcgctccac gcgaccgctc acgccgccaa
    24661 tgcgcacgtc cgtccccacg tccagcgcgt gctcgggcgc ctccacccag aagctgccgt
    24721 cccgaccgtg cgcccgtccc acgcgaccca cggccacgtg tcgctgctcg gtcaatccac
    24781 cacgtcgacg aggacccggc agccgtgctt gaccgccgac gccctgatca cggtgcgcac
    24841 cgcgttgatc gtccggcctc cgcggccgat cagcttgccc acgtcgtcct cgccgacgga
    24901 gagctccagc acgaccgtgc cgtcgtcctc ctcgaaggcc tcgaccgaga cttcatccgg
    24961 ctcgtcgacg agcgagcgcg ccaggtactc gaggagatcc tccatcgagc tctcagccgg
    25021 tcgagtcgcc cgcgccggcc tgctggaacc cggtcgcgcg catcagcttc ttcacggtgt
    25081 cgctcggctg cgctccgcgg tcgagccagt gccggagacg ctcctgatcg aggacgatct
    25141 ccgacggctc cacctgcgcg ttgtagcggc cgatggtctc gatcacgcgg ccgtcacgcg
    25201 gcgagcgctg gtctgcgacg accacgcgcc agatcgggtt cttcttgctg cccacgcggg
    25261 tcagcctcac acgtactgcc aagtctcagg tcacctcggt ttcttgaaag agcgggcgga
    25321 acgttagcgg ccccgcgcga gctgctgtag cgacggcatc ttcccctggg acagctggcg
    25381 catgaccttc tgcatctggc cgaactgctt caccagttgg ttgaccgcgc tcacgttggt
    25441 tccggagccc tgcgcgatgc gcttccggcg cgagccattc aggagcgccg ggttcgcgcg
    25501 ctcctccgga gtcatcgagg tgatgatcgc cgcgatccgg tcgagctccc gctcgtcgac
    25561 cttgagattc ccgagctggc tgcccatccc ggggatcatc ttcatgatgc tcgtgagcgg
    25621 gcccatccgg cggagctggt gcagctggtc gaggaagtcg tcgagaccga actgctgctt
    25681 gcggatcttc cgctcgagct cctcggcctg gcgctcgtcc atctgctgct cggccttctc
    25741 tatgagcgtc ctgacgtcgc ccatcccgag gatccgctgt gccatgcggt ccgggtggaa
    25801 gcgctcgaac tgctcgagct tctcgccggt ggacgcgtag agaatcggct tgccagtgac
    25861 ggccttcacg gacagcgcgg cgccgccgcg cgcgtcgccg tcgagcttcg agaggacgac
    25921 cccgtcgaag ttcgccacct cggcgaactg ctcagccacg ttcacggcgt cctgaccggt
    25981 catggcgtcg accaccaaca gcacgtcgtg cggcttcgtc cgcttgcgga tcttggcgag
    26041 ctcgtccatc agcgcctgat ccacatgcag ccgcccggag gtgtcgatga tcacgacgtc
    26101 cttgccctcg gacttcgcgc ggttcacggc ccattcggcg atgtcgaccg ggtcgcggtc
    26161 cgtcccctgc tcgtacacgt ctgcgccgac ctgcgagccg agcttcacga gctggtcgac
    26221 cgccgccggc ctatacacgt ctgcggcgac gagcgcgacg ctctttccct cctcctcctg
    26281 caggtggcga gcgagcttcg cggtggcggt cgtcttgccg gagccctgga ggcccgccat
    26341 caggatcacg gtgggcgcgc gcgggctgat cacgaggtcc cggccggcgc cgcccatcag
    26401 ctcggtgagc tcgtcgccga cgatcttcac cacctgctgg cccgggttga gcgactgaag
    26461 cacgtcggcg ccgagcgcgc gctccttcac gtcggcggtg aagcgcttca ccacctggaa
    26521 gttcacgtct gcctcgagca gggcgaggcg cacctggcgc atcgccttgt tgacgtcgtc
    26581 ctcggtcagc ttcccgcggg agcggacgtc gtcgagggct gcttcgagac gctcggaaag
    26641 ggcttcgaac atggcctgat gaggctagcg ggcgcgcgca gctacgaacg ggccgtgtcg
    26701 agcgcgcgaa gcacgtcgcc aaccacgtcg gcggtctcct cgcaaccggc gctgaagcgg
    26761 atgaagccct cggagaccgc gtcgcccgac cagcgggcgc gccgctcggc gctggtgtgc
    26821 acaccgccga agctggtcgc gtcggacacg agccggcact cggcgaggaa gcggtcggcg
    26881 cgctcctggc tgcccacgtc gaagctgaga atcggcccga agcgggtcat ctggcgtgcc
    26941 gctagctcgt gcgacggatc gccctcgagc ccgggatagc ggaccgcgct cacgtcgtcg
    27001 cgctcggcga gcgcctgcgc gagcgcgagc gccgtcgcgc actggcgctc gagccgcacc
    27061 ccgagcgtcg ccagcgaacg gtgcgcgagc cagacctcga acggtcccgg cacactgccc
    27121 gccatcgtcc gccaggcggt gatcgctggg tgcgcggagt cgcgggtcgc tacgtagccc
    27181 aggatcaggt ccgcatggcc ggtcagcatc ttcgaggcgc tcgcgaccga gaagtcggcg
    27241 cccagctcga gtggccgctg cccgagcggc gttgcgaacg tgttgtcgac ggccaccagc
    27301 gcgccccgct cgtgagcggc gtcggcgagc gcgcggatgt cgcacacctc gagctgcgga
    27361 ttagtgggcg tctcgagcaa caggaacgtg gcgccgtcga gctgagccgc catcgcgtcg
    27421 cccggcgtag gtgcgagccg cacctccacc cccatcggct gcaggtagcg ctccgccagg
    27481 atgcgcgtgc cgcggtagga gtccgcgggc gccacgagca cgtcgccggg gcggagccgc
    27541 ggcaggagca gcgcggacag cgccgccatg cccgaggcga acaccacggc ggcggcgcca
    27601 ccctcgagct cggcgagcgc ggcctcgtag cgcgaccacg tcgggttccc gtagcgcccg
    27661 tagacgaagt cggagtcctc cggtcggccg cgcaggtggt agggcgcggc gaatgtcgga
    27721 cccgggagga acggctcgcc ctggccggcc tcgggcaggc ccgcacgcac cgcgcgcgtg
    27781 ccgtcgccaa agtcctcgcg cgccacgact acgcctcgag gatcgcgcgg gcgaagtcgt
    27841 cggcgtcgaa cgggcgcagg tcctcgagcg actcgccgat cccgatcagc ttcaccggca
    27901 cgccgagctc ctgagcgatc gcgagtgcga tcccgccctt ggcggtcccg tcgagcttgg
    27961 tcagcacgat cccgtccacg tcgaccgcct cacggaagag cagcgcctgc cgcaagccgt
    28021 tctggccggt ggtggcgtcg atcgtgagca gcgtctcgtg cggagcgtcg gggatcagct
    28081 tctggatcac gcgccgcacc ttcgcgagtt cgtccatcag gtgatcctgg gtgtgcagac
    28141 gcccggcagt gtcgatgatc acgacgtcgt ggccgcgcgc tcgggccgcc tcgatcgcgt
    28201 cgtaggcgac cgcgccgggg tccgagccct gcgccgcctt gacgatgtcc gtaccggcgc
    28261 gctcggccca ggcggtgagc tgctccacgg cagccgcccg gaacgtgtct ccggcagcga
    28321 gcagcaccga cttgccgagc tcgttctgca ggtgccacgc gatcttcccg atcgtcgtcg
    28381 tcttgcccgt gccgttcacg cccaccatga ggatcacggt cgggtcgtgg cgcaagtcga
    28441 tcgtcgcgtc cccggtgcgc gcggactccg ccagcagctc gcgcaggcgg tcgctcagct
    28501 cctcgccggt cgagaggtct ccggaagctg cctcggtctc gagccgctcg acgatcctcg
    28561 cggtcgtcgg cgcacccacg tcggcgtaga tgagcgtctc ctcgagccgc tcccaggcct
    28621 cgtcgtcgag gttctggaac acgctcgcct gcagctccgc gccgagcgcc tggcgagtct
    28681 tcgacatgtt gtcgcggagc ctccggaaga agccgcgacg ctcctcctgc gcctcggagg
    28741 cgtcggtctc gggcgccggc gcatcgccgg tcacgaacag ctctgtccac gttcgcggca
    28801 tggccgcgtg aggataggcc ctgcgagcta ccggctgggc gccgcgctag gcagcggcct
    28861 cgtcgcccgc gggaatggcc gcgagcttgc gcgacaccac cttggatacg ccgtcgccgc
    28921 ccatgctcac cccgtagagg cagtcggctg cgtccatcgt ccgcttctgg tgtgtcacga
    28981 cgatgaactg cgcgcgattg gagaactggc gcacgagctg caggaagcgg tcgatgttga
    29041 ggtcgtcgag cgccgcctcc acctcgtcga ggatgtagaa cgggcacggc cgggcgagga
    29101 acacggcgaa caggaacgcg agcgccacga gcgacttctc ccctcccgag agaagcgaca
    29161 gccgcttcat cgacttgccg gccggcgtga cctcgacctc cacgccgggc tcgtcgaccg
    29221 cgggggcctc atccgggtcg ggctcggccg cgacgacgtc gcccggctcg gcgccgccct
    29281 cctccgccga gtccacagcg ggcttcccgt cctcgccgcc gatcacggga cgcgggccgt
    29341 ccgggtgcac gaggcgcaaa cggccgcggc cgcctgggaa caggtgctga acgacctcct
    29401 cgaagttctt cgccgccgcc tcgaaggtct cctcgaagct ctctcggatt cgcttgtcgg
    29461 tctccttgat gagcccgtct agctcggcga gcgccgcctc gaggtcgcgc cgctgctcct
    29521 cgagctcctc gacgtgcgcg actgcctcct cgtactcctg cttcgcgagc gggtttacag
    29581 gaccgagctg ctcgcggcgc cgcgcgagcc gctccacgcg cgattcgagc tccgcttggc
    29641 gctcctccgc cagcggctcc gcggccggcg ccgggtcgag cccgaggcgc tcggcgagcc
    29701 gctcgagctc cgagccggcc tcggcgccga agtcgcgcgc ctgctgcgcg cgcacctccg
    29761 cggcggtcac ctcctgcgat gcctcgcgga gcttctcctg cagccctgcc tcctggaggg
    29821 cgcagacgcg cagctccgcc gccgtcgcct cgcccgcctc gtgatcggcc tcgagctcgg
    29881 ccgtgagctg gtcgcgccgc gcggcgaccg cctcggcggc ggccgaaagg gccgcggccg
    29941 cgcgctcggc cgcctgcgct agcgagtcat cgcgctcgat ccgtgcgcgc aggcgctcga
    30001 tgcgcgcagt gcgctcggcc tgctcccgcc gcgcctgctc gagcagccgg cgctcggccc
    30061 gcagctcggc ggcgagctct gcgtggcgaa cgtccgcggg cccgccggtg ggctcctcgc
    30121 gccgcctcgc gatcgcgcgc tcgaggcggc tgaccgcctc ctgcgcgtcc tc
//
LOCUS       DASVXS010000035         9615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_9122, whole genome shotgun sequence.
ACCESSION   DASVXS010000035 DASVXS010000000
VERSION     DASVXS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9122"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF752_01890"
     CDS             complement(<1..530)
                     /locus_tag="VF752_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX8854323.1"
                     /translation="MSRLRHYGPGARAAIPLAIAVGVFGVSFGVLAQKAGMGTLAPIV
                     MSVITFAGSAQFAAVSLLSTGAGVAAACIAAILLNLRYLPIGVSAASALKGGLLKRFA
                     EAQLVVDESWALGQPDPGRFDRRLMIGAGLLLYFAWVVGTTVGVLAGDLLGDPEKLGL
                     DAAFPALFLALLAGQLR"
     gene            complement(527..1390)
                     /locus_tag="VF752_01895"
     CDS             complement(527..1390)
                     /locus_tag="VF752_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8854324.1"
                     /translation="MRRHTFGSLLIASCALSWGFIGIVVREIALPPLLIVFFAALLSA
                     AAIAAFLALTGRRRRLRPPGRPVLILGLLLGGHWCSYFAAINETSVASAVLVTYAGPV
                     IMALIAPALIGERVPSVTVGALAVSVAGIAAISLSGSSGGVHLLGGLLALVAAITFAL
                     QVVMLKRYTLEMDPLSVQLWESIVVAIALAPVAILTSWSLHGSDVGYIVLLGIVLTGF
                     TGAIYIASLRWVAVTTAGILGYLEPVTAAIFASLLLGQELTVAVIIGGVAIVASGIAV
                     VVKAAEPVAVR"
     gene            complement(1403..2059)
                     /gene="cpaB"
                     /locus_tag="VF752_01900"
     CDS             complement(1403..2059)
                     /gene="cpaB"
                     /locus_tag="VF752_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8854325.1"
                     /translation="MIALAVVCGGLAASRVGSREAEVEAQIGKPVSVLVARADIPAGR
                     RIGSEMLGVRRVPARFAPPDALIDPAEARSLRAAVALERGAYITASSLTDRAAPVAAG
                     SPLRRGERAVQVAVAGGETLAGAAPGARVDVLVTSEPRSGAGRTWVALEDVELLALRS
                     GGASLETAKGSATTLATLRVTLRQAVFLTAAQSFSRELRLLPRPPGEPRRGMRIEASL
                     "
     gene            complement(2086..2745)
                     /locus_tag="VF752_01905"
     CDS             complement(2086..2745)
                     /locus_tag="VF752_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8854326.1"
                     /translation="MTALLLCGWALAVAAAARCVILGRRLDLVALAAHELRSPLTVIG
                     LELERLSGPRARRLETQMQRVRLGLADLDCARTGRRAAARARLVELGELLGRAGAGWE
                     RAASQAGGSLRLRWGAGRARAHVHDGRLLQALDNLVANAVEHGGPRLELVGERREDRV
                     RVELRDSGGGFDSGPPPDRRAGRGRGLEIARRAVEDSGGRLEVGPGAKVAVELPLASE
                     R"
     gene            complement(2742..3452)
                     /locus_tag="VF752_01910"
     CDS             complement(2742..3452)
                     /locus_tag="VF752_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854327.1"
                     /translation="MIEDRKSLLVVEDDAATAEFLSDNLAVDGFDVTTAGGAGEAIRR
                     MQVSAPDLLVLDLVLEDGSGFIVLDQVRAADGLASRIDPDLPVLVLSGRSSDVDRVRG
                     FARGADDYLVKPFSYAELVARVHALLRRAGLRRHAGVIRVGDLTLDPSTRAVRLAGEP
                     IELSPKEFALLHALAQEPDRVLTKHELLRDVWGYRAYGATRTLDAHACRLRKKLAASP
                     RPFVLNVRGVGYRLVERA"
     gene            3602..4063
                     /locus_tag="VF752_01915"
     CDS             3602..4063
                     /locus_tag="VF752_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854328.1"
                     /translation="MDLTKRGNYESGSDYVLEYGELRFTFNERDFTERVEQAAVKLGF
                     VDGPLESDEREDLVNLAVNGEVIDPASELGEHINTCWGELHGASERSLVHWLRRLVFR
                     GAWLDQRVKEGELEIVFDSDTHSFGYAQPDRGWELVELSPEPSWASIAYRR"
     gene            4109..4387
                     /locus_tag="VF752_01920"
     CDS             4109..4387
                     /locus_tag="VF752_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX8854329.1"
                     /translation="MPDAVEQDHHGKPSFRVGGKIFATLWSPNEMNVMASEPRIRMAV
                     ADDPDVCSEVMWGRKLAAVRVKLDAASEALLEDLLGEAWAQRAPKQQQ"
     gene            complement(4524..5708)
                     /locus_tag="VF752_01925"
     CDS             complement(4524..5708)
                     /locus_tag="VF752_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX8854330.1"
                     /translation="MSVLDRKELEQSPLADLHAIAAEVGVEGFRRLRRDDLIAAILGE
                     AGGDGAEDRADDEEEAEPRGRRRGRRGGRARRARSSDPGDVADVEELEAEIAEEPEEE
                     APAGVEAEEPEPEEPEEELVRTGLLDILPNGSGFLREDPFSHSREDVYVSPAQIRRCE
                     LRAGDEVTGPVRPPRRSERYPSLIRVDAVNGNAPEPASERPRFEELTPVFATERLSSP
                     DELASIAYGKGSRVALSGPPGAGITTLLRHIASTLAEKHSDLDVTVILVGVRPEEVTE
                     WRRDSGLRVAGGGFDAAGEHQSQAAEMAVELAKRTVEKGRDAVLVVDALDALPPGVAR
                     RVFGAARNAEEGGSLTVFAAIGAAHEALRVATTRILLEAAPGGGAQLAPGSGTTHADL
                     LS"
     gene            complement(5712..6407)
                     /gene="fsa"
                     /locus_tag="VF752_01930"
     CDS             complement(5712..6407)
                     /gene="fsa"
                     /locus_tag="VF752_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX8854331.1"
                     /translation="MKLFIDTGSTAEVEEIAAWGVLSGATTNPSLLAKEEGDPGDIIR
                     RICELVDGPTSAEVVSDDARGMVQEGRALARLHEHIVVKTPFSKDGLAACNELSEDGI
                     RVNMTLVFSANQALLAAEAGATYISCFMGRLDDISVDSEAVVAEIVSALRPSGTQSQV
                     LAASLRHPGHVIQAAQLGCEVATAPAKVLKAMLDHPLTTAGIERFKADWETRPEFAEW
                     LRGLVEQQAVTAS"
     gene            complement(6404..7300)
                     /locus_tag="VF752_01935"
     CDS             complement(6404..7300)
                     /locus_tag="VF752_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854332.1"
                     /translation="MSETIVYKRDHGLPYSKGLMAQSLSASGLSPGRAFELARVVERR
                     LDKRGDGRIDVQSLYALVEDVLAEEEGEGAVRRFHQWQRLGNLDQPLILLLSGTTGVG
                     KSTLATMAANRLGITRVIATDVIRQVLRAFFSHDFMPSVHYSAFEAGSAVESLSGEQG
                     DPDLIGFAQQAEAIATGVDAIVERACLERTPMVVEGVHLVPGSLGPEIRGRCVAVEAV
                     LVVGDEELHRAHFSMRGGHRPAQRYLEHFEQIRKLQDYLVERGRKRGVAVIENESIDQ
                     ALPQLMDLVLESVGRVSSSNAE"
     gene            complement(7293..8543)
                     /locus_tag="VF752_01940"
     CDS             complement(7293..8543)
                     /locus_tag="VF752_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854333.1"
                     /translation="MPGDGPVIALVDGEHHPSAVREALDRVQSDRGLAAVVFCGGEEK
                     VRTEALEDPYRYYGRSIERGDPAEVVERLARTTGAVTVLDLADEPVLAAAAKMRLACH
                     ALNGGLSFEGPGFRVRAPVYERVAFEGPKLAVIGTGKRTGKTAVAGHWATLLRERGAA
                     PVIVSMGRGGPAEPQLAGADTSLDDLLEIAANGLHAASDYLEDAAIARVPAVGCRRVG
                     GGLAGEAYTSNVLAGAELARSLDPSALIFEGSGATIPPVEVNRTVCIVGERSEALGEL
                     GPYRLLRAQLALVMRGDSELARDVAEICRGRVLTCELRAEPTEEVPRDARVAAFTTGA
                     PPPSGAIVESTNLARRSTLEADLERAVRERCDVWLVEIKAAGIDTVAERARREGARIV
                     FLRNRPVALDGDLDAELVKLHADV"
     gene            8590..9000
                     /locus_tag="VF752_01945"
     CDS             8590..9000
                     /locus_tag="VF752_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854334.1"
                     /translation="MARDLAWFQDLYARGTRPEAVPDCWTEDVVVIQSSDIPDTAGTF
                     RGHAGVLAAGREMAESVRGMTWVLSEVQELGGDRYLVLLHMRGEGQGSGVPVDVEAGH
                     IVTLDGDRIARLEAFLGWDTARTVAAARAGPGLA"
     gene            9089..>9615
                     /locus_tag="VF752_01950"
     CDS             9089..>9615
                     /locus_tag="VF752_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX8854335.1"
                     /translation="MARSETPGEGSGDATRSDAQTTREGREGGAGPDSVRATRRIPSS
                     LVSPVERDRMVERARDVEFPSALRGYEREAVDRYVEQVNRLISELELSSSPESAVRHA
                     LEEVSEETRELLQRAHETADEITMRSRSKADDRLQQAEKEAAEAREAASRDVQETRDA
                     AAREVQELTERARREA"
BASE COUNT         1514 a         3476 c         3359 g         1266 t
ORIGIN      
        1 cggagctgac cggcgagcag cgcaaggaac agtgccggga aggcggcgtc gagacccagc
       61 ttctccgggt cgcccagaag gtcgccggca agcacaccga cggtcgtgcc gacgacccag
      121 gcgaagtaga gaagaagtcc cgcgccgatc atcagccgcc tgtcgaagcg gcccgggtcg
      181 ggctgtccga gcgcccacga ctcgtccacc accagctgag cctccgcgaa gcgcttgagc
      241 aacccgccct tcagcgccga cgcagccgac acgccgatcg gcaggtagcg caggttcagc
      301 aggatggccg cgatgcacgc tgccgccact ccggctccgg tgctgagcag cgaaacggcg
      361 gcgaactgag ccgaccccgc gaaggtgatc accgacatga cgatcggcgc gagagtgccc
      421 atgccggcct tctgcgcgag tacgccgaac gagacgccga acacgccgac cgcgatcgcg
      481 agcgggatcg cggcgcgcgc gccgggccca tagtggcgaa ggcggctcac cggacggcga
      541 ccggctcggc agccttcacg accacagcga tgcccgaggc cacgatcgcg acgccgccga
      601 tgattaccgc gaccgtgagc tcctggccga gcagcagcga cgcaaagatc gccgctgtga
      661 cgggctcgag gtatccaagg attcccgcgg ttgtgaccgc cacccagcgc agcgaagcga
      721 tgtagatcgc gccggtgaag ccagtgagca cgatgccgag caggacgatg tagccgacgt
      781 cggagccgtg cagcgaccag ctagtgagga tcgcgacagg cgcgagagcg atcgccacca
      841 caatcgactc ccatagctga accgacagcg ggtccatctc gagcgtgtag cgcttgagca
      901 tcaccacctg gagcgcgaac gtgatcgcgg ccaccagcgc gagcagaccg ccgagcaggt
      961 gcacgccgcc ggagcttccc gaaagcgata tcgcggcgat gcccgccacc gacaccgcga
     1021 gggcaccgac ggtgacactg ggcacccgct ctccgatcag cgccggcgcg atcaatgcca
     1081 tgatcaccgg cccggcgtac gtgacgagga ccgcgctcgc caccgaggtc tcgttgatgg
     1141 ccgcgaagta ggagcaccag tggccgccta gaagcaggcc gagaatgagc acgggacggc
     1201 ccggcgggcg cagtcgccta cgccgaccag tgagcgcgag gaacgccgcg attgccgcgg
     1261 ccgacaggag cgccgcgaag aacacaatca agagcggtgg gagggcgatc tcgcgcacga
     1321 cgattccgat gaagccccaa ctgagggcgc acgaggcgat caggagcgat ccgaaggtgt
     1381 ggcggcgcat cggggctgca cgctacagag aggcctcgat ccgcatgccg cggcgcggct
     1441 cgccgggcgg ccgcggcagt aggcgcagct cgcgcgagaa gctctgggct gcggtgagga
     1501 acaccgcctg gcgcagggtg acgcgcaggg tcgcgagcgt ggtggccgac cctttggccg
     1561 tctcgagcga cgcgccaccg cttcggagcg cgagcaactc cacgtcctcg agcgctaccc
     1621 acgtcctgcc cgctcccgag cgcggctcgc tcgtcacgag cacgtccacg cgcgcgccgg
     1681 gggccgctcc ggcaagcgtc tcaccgccgg cgacggccac ctgcaccgca cgctcgccac
     1741 ggcgcagcgg cgagccggcg gcaaccggcg ccgcgcgatc cgtgagcgat gacgccgtga
     1801 tgtaggcgcc ccgctcgagc gcgacagccg cgcgcagcga acgggcctcg gcgggatcga
     1861 tcagggcgtc cggcggcgcg aaccgcgccg ggacgcggcg gacaccgagc atctccgaac
     1921 cgatccgccg gccggccgga atgtcggcgc gcgcaacgag cacggacacc ggctttccga
     1981 tctgcgcctc gacttcggcc tcgcgactgc cgacgcgcga cgccgccaag ccgccacaga
     2041 ccacggcaag cgcgatcaac gtcagcgctc tcctgcgccg ggtgctcacc gctcggaggc
     2101 gagcggaagc tccaccgcga ccttcgcgcc cggaccgacc tcgagcctgc cgcctgagtc
     2161 ctccacggcg cggcgtgcga tctcgagacc acggccgcga ccggctcgtc gatccggcgg
     2221 cggtccagag tcgaacccgc cgcccgagtc gcgaagctcg acgcggaccc tgtcttcgcg
     2281 ccgctcgcca acgagctcca gccgcgggcc gccatgctcg accgcgttcg ccaccaggtt
     2341 gtcgagcgcc tgcaggagcc gcccatcgtg cacgtgggca cgggcgcgtc cggcgcccca
     2401 gcgcaggcgg aggctgccgc cggcctgcga ggcggcgcgc tcccagccgg caccggcgcg
     2461 ccccagcaac tccccgagct ccacgaggcg ggcccgcgcg gctgcgcggc ggccggtgcg
     2521 cgcgcaatcg aggtcggcga ggccgagcct cacgcgctgc atctgcgtct cgaggcgccg
     2581 agcacggggc ccggacagcc gctcgagctc caacccgatc accgtcagcg gcgagcgcag
     2641 ctcgtgggcc gccagggcga caaggtcgag ccgacgcccc aggatcacgc agcgggcggc
     2701 tgcggctacc gcgagcgccc agccgcagag gagcagcgcc gtcatgcacg ctccacgagc
     2761 cggtagccga cgccgcgcac gttgagcacg aacggccgcg gcgacgccgc gagcttcttc
     2821 ctcagccggc aggcgtgggc gtcgagcgtg cgcgtggcgc cgtaggcgcg gtagccccag
     2881 acgtcgcgga gcagctcgtg cttggtgagc acccgatcag gctcctgcgc aagtgcatgg
     2941 agcagcgcga actccttcgg ggagagctcg atcggctcac cggcgaggcg gaccgcgcgc
     3001 gtggacgggt cgagcgtgag gtcgccgacg cgaatcacgc cggcgtggcg gcgcaacccg
     3061 gcgcggcgca gcagggcatg cacgcgcgcg acgagctcgg cgtacgagaa cggcttcacc
     3121 agatagtcgt ccgccccgcg ggcgaagccg cgtacgcgat ccacgtcgct cgagcgcccg
     3181 ctcaggacga gcaccggcag gtccggatcg atccgcgagg cgaggccatc ggctgcacgt
     3241 acctggtcga gcacgatgaa cccgcttccg tcttccagca cgaggtcgag gacgagcagg
     3301 tctggcgcgc tcacctgcat gcgcctgatc gcctcgccgg ccccgccggc ggtcgtcacg
     3361 tcgaagccgt cgaccgccag gttgtcggac aggaactcgg ccgtggcggc gtcgtcctcc
     3421 accacgagca gcgacttgcg gtcttcgatc acacctgcct acgtcgcgcg gcggccgatt
     3481 tcgcccccct cggctgcaaa tccctttgca ccccttctgg cgagagagga atccggctta
     3541 tgatgccgac gccgtggcgc ccggggtttg ggggtaccgg agcctgggag ggcaacgact
     3601 gttggatctg accaagcgag gaaattacga gtccggttcg gactatgtgc tcgagtacgg
     3661 cgagctgcgc ttcacgttca acgagcgcga cttcaccgag cgcgtggagc aggccgccgt
     3721 gaagctcggc ttcgtggacg gtccgctcga gtcggacgag cgcgaggacc tcgtgaacct
     3781 ggccgtgaac ggcgaggtga tcgaccccgc gtccgagctt ggtgagcaca tcaacacgtg
     3841 ctggggcgag ctgcacggcg cgagcgagcg ctccctggtg cactggctcc gtcgcctggt
     3901 gttccgtggc gcctggctcg atcagcgcgt gaaggagggc gagctcgaaa tcgtgttcga
     3961 ctccgacacc cacagcttcg gctacgcgca gcccgaccgc ggctgggagc tcgtggagct
     4021 gtctcccgag ccgagctggg cctcaatcgc gtaccgccgt taggtcgccg tgcttagcgc
     4081 ggacgacgcg cgtcggctgg ctctctcgat gcccgacgcg gtcgagcagg accaccatgg
     4141 caagccctcg ttccgggtag gcggcaagat cttcgccacg ctctggagcc cgaacgagat
     4201 gaacgtgatg gcgagcgagc ctcgtatccg gatggcggtg gccgacgatc cggacgtgtg
     4261 ctcagaggtg atgtgggggc ggaagctcgc cgccgtgcga gtgaagcttg acgcggcctc
     4321 cgaggcgctg ttggaggatc tcttgggcga ggcctgggcg cagcgcgccc ccaaacagca
     4381 gcaatgagga gagcgcgctc tcgtttcggc ggacctgggc cgttttgacc atgactgtca
     4441 tggtcaaaac agcccacccc accgggctct ccctaagtgc tcagcagccc cgcccgtccc
     4501 gcgccgcgca ccgcggacga gcgctaactc agcagatcgg cgtgcgtggt gcccgaaccg
     4561 ggcgcaagct gcgcgccccc gccgggtgcc gcctcgagca ggatgcgcgt ggtcgccacc
     4621 cgcagcgcct cgtgcgccgc cccgatcgcc gcgaagacgg tcagcgagcc gccctcctcc
     4681 gcgttgcgcg ccgcgccgaa cacgcgccgc gccacacccg gcggcagcgc gtcgagggcg
     4741 tcgacgacca acaccgcgtc gcgccccttc tcgacggtcc gcttcgcgag ctccacggcc
     4801 atctcggcgg cctgcgactg gtgctcgccc gcggcgtcga agcctccgcc ggccacgcga
     4861 agcccggaat cgcgccgcca ctcggtgacc tcctccgggc gaacgcccac gaggatcacc
     4921 gtgacgtcga ggtcgctgtg cttctcggcg agcgtgctcg cgatgtggcg cagaagcgtc
     4981 gtgatgcccg ccccgggcgg gcccgagagc gccacgcgtg accccttgcc gtacgcgatc
     5041 gacgccagct cgtcaggcga gctgagccgc tcggtcgcaa agactggcgt gagttcctcg
     5101 aagcgaggac gttcagacgc cggctcgggg gcattgccgt tcaccgcgtc cacgcgaatg
     5161 agcgacgggt agcgctcgga gcggcgtggc gggcgcaccg ggccggtcac ctcatcgccg
     5221 gcgcgcagct cgcagcggcg gatctgcgcg ggcgacacgt acacgtcttc gcgcgaatgg
     5281 gagaacgggt cctcgcgcag gaagccgctg ccgttcggaa ggatgtcgag caagccggtg
     5341 cgcaccagct cctcctcggg ctcctcgggc tccggctcct cggcctcgac gcccgcgggc
     5401 gcctcctcct cgggctcctc ggcgatctcg gcctcgagct cctcgacgtc cgcaacgtca
     5461 cctggatcgg acgagcgcgc ccggcgcgcg cggccgccgc gccgtccccg gcgacgaccg
     5521 cgcggctcgg cttcctcctc gtcgtcggcg cgatcctccg cgccgtcgcc gccggcctcg
     5581 ccgagaatcg ccgcgatcag gtcgtcgcgc cgcaggcggc ggaagccctc cacccccacc
     5641 tccgcggcga tcgcgtgcag atcggccagg gggctctgct cgagctcctt acggtcgaga
     5701 accgacatgg gctaactcgc tgtgaccgcc tgctgctcca cgagcccgcg gagccactcc
     5761 gcgaactcgg ggcgggtctc ccaatcggcc ttgaagcgct cgatgccggc cgttgtgagc
     5821 gggtggtcaa gcattgcctt cagcaccttc gccggcgcgg tggcgacctc gcagccgagc
     5881 tgcgcggcct ggatcacgtg acccgggtgg cggagcgacg ccgcgagcac ctgcgactgg
     5941 gtgcccgacg gacgcaatgc cgacacgatc tcggcgacga cggcctccga gtcgaccgag
     6001 atgtcgtcga ggcggcccat gaagcaagag atgtacgtgg cccccgcctc agcagcgagc
     6061 agcgcctggt tcgcggagaa cacgagcgtc atgttcacgc gaatcccgtc ctccgacagc
     6121 tcgttgcacg cggcaagccc gtccttcgag aacggcgtct tcaccacgat gtgctcgtgc
     6181 aggcgcgcca gcgcgcggcc ctcctgaacc atgccgcgcg cgtcgtcgga cacgacctcc
     6241 gcggacgtgg ggccgtcgac cagctcgcag atgcggcgga tgatgtcgcc gggatcgccc
     6301 tcctccttcg ccagcagcga cggattggtg gtagcgcccg agagcacgcc ccaggccgcg
     6361 atctcctcta cctctgcagt tgagcctgtg tcgatgaaga gcttcattcg gcgttcgatg
     6421 atgacacacg gccgacggac tcgagcacga ggtccatgag ctgcggcagc gcctggtcga
     6481 tgctctcgtt ctcgatcacc gcgacaccgc gcttgcgccc gcgctcgacg agatagtcct
     6541 gcagcttgcg gatttgctcg aagtgctcga ggtagcgctg ggcgggccgg tgacccccgc
     6601 gcatggagaa gtgcgcccgg tggagctctt catcgcccac gaccagcacc gcctcgacgg
     6661 cgacgcagcg gccgcggatc tccggcccga ggctgccggg cacgagatgg acgccctcca
     6721 cgaccatcgg tgtgcgctcg agacaggcgc gctccacgat cgcgtcgact cccgtcgcga
     6781 tcgcctcggc ctgctgtgcg aagccgatga ggtcggggtc gccctgctcg cccgagagcg
     6841 actcgaccgc gcttccggcc tcgaacgccg agtagtgcac cgagggcatg aagtcatgcg
     6901 agaagaatgc cctcaggacc tgacggatca cgtcggttgc gatcacccgc gtgatgccga
     6961 ggcggttggc cgccatcgtc gcgagcgtgg actttcccac cccggtcgtg cccgacagca
     7021 gcaggatcag cggctggtcg aggtttccga gccgctgcca ctggtggaag cggcggacgg
     7081 cgccctcgcc ctcctcctcg gcgagcacgt cctccacgag cgcgtagagg ctctgaacgt
     7141 cgatgcggcc gtctccgcgc ttgtccagcc ggcgctccac cacgcgcgca agctcgaacg
     7201 cgcgccccgg cgagaggccg gacgccgaca gcgactgcgc catcagcccc ttcgaatagg
     7261 gcagaccgtg atcgcgcttg tagacgatcg tctcagacat cggcgtgaag cttgacgagt
     7321 tccgcgtcga ggtcgccgtc gagcgcgacc gggcggtttc gcaggaacac gatccgcgcc
     7381 ccctcgcgcc gcgcgcgctc cgcgaccgtg tcgatgcctg ccgccttgat ctccacgagc
     7441 caaacgtcgc agcgctcccg caccgcgcgc tcgaggtccg cttcgagcgt ggagcggcgc
     7501 gcgagattcg tcgactcgac gatcgcgccc gacggcggcg gcgcgcccgt ggtgaacgcc
     7561 gccacccgcg cgtctcgcgg cacctcctcc gtgggctccg cacgaagctc gcacgtgagc
     7621 acgcgaccgc ggcagatctc ggccacgtcg cgcgcgagct ccgaatcgcc gcgcatcacg
     7681 agcgcgagct gcgcgcgcag cagccggtac gggccgagct cgccaagcgc ctcggagcgc
     7741 tctccgacga tgcacacggt gcggttcacc tcgacgggcg ggatggtcgc cccggagccc
     7801 tcgaagatca aggcgctcgg gtcgagcgag cgcgcaagct ccgcgcccgc gagcacgttc
     7861 gacgtgtacg cctcccctgc gagcccgccg cccacgcgcc ggcagccaac tgccggcacg
     7921 cgcgcgatcg ccgcatcctc caggtagtcc gaggccgcgt gcagcccgtt cgccgcgatc
     7981 tcgagcagat cgtcgagcga cgtgtcggcg cccgcgagct gcggctcggc cggtcctccc
     8041 ctgcccatcg agacgatcac cggagcggcg ccgcgctcac gcagcagcgt cgcccagtgg
     8101 cccgcgaccg cggtcttgcc ggtgcgcttg ccggtgccga tcaccgcaag cttcggacct
     8161 tcgaacgcaa cgcgctcgta cacgggagcg cgcacacgga acccgggtcc ctcgaacgag
     8221 agcccgccgt tcagcgcgtg gcaggcgagc cgcatcttcg cggccgccgc gagaaccggc
     8281 tcgtcggcga ggtcgagcac cgtcaccgcg ccggtcgtcc gcgcgagcct ctccaccacc
     8341 tcggccgggt cgccgcgctc gatcgagcgc ccgtaatacc ggtacggatc ctcgagcgcc
     8401 tcggtacgaa ccttctcctc cccgccgcag aacaccacgg ccgcgaggcc ccggtcggac
     8461 tgcactcggt cgagcgcctc gcgcaccgcg ctcgggtggt gctccccgtc caccagcgca
     8521 atcaccggcc cgtcgcctgg cacgcggcgc attcttgctg gtcgtgaggc ggtggtctac
     8581 aactgcggca tggcccgcga cctggcctgg ttccaggatc tgtacgcgcg cggcacgcga
     8641 ccggaggcgg tccccgactg ctggaccgaa gacgtcgtgg tgattcagag cagcgacatt
     8701 cctgataccg cgggcacttt ccgcggccac gcaggcgtgc tggctgcggg ccgagagatg
     8761 gccgaatcgg tgcgcgggat gacttgggtg ctcagcgaag tgcaggagct cggcggcgac
     8821 cgctacctcg tgctcctgca catgcgcgga gagggtcagg ggagcggcgt gccggtggat
     8881 gtggaggccg ggcacatcgt gacgctcgac ggggaccgga tcgcccggct ggaggcgttc
     8941 ctcggctggg acacggcgag aacggtcgcg gctgccaggg ctgggccggg tcttgcatag
     9001 ctctcttgac caggggagta gcgtcgcggt agacgcttgt ggcctcacga cgggggccct
     9061 gaccgcgacc agcacaggag ggtggacgat ggcacgttcc gaaactcccg gcgagggctc
     9121 cggcgacgca acgaggagcg atgcccagac cacgcgtgag gggcgcgaag gcggggcggg
     9181 acccgactcg gtccgcgcca cacgacgcat cccgagctcg ctcgtgagcc cagtggagcg
     9241 cgaccggatg gtcgagcggg cgcgggacgt tgagttcccg agcgcactgc ggggttacga
     9301 gcgcgaagct gtcgatcgct acgtggagca ggtgaatcgc ctgatcagcg agctcgagct
     9361 gtcctcttcg ccagagtcgg cggttaggca cgcgctcgag gaggtgagtg aggagacccg
     9421 cgagcttctc cagcgtgccc acgagacggc cgacgagatc accatgcggt cgcgctcgaa
     9481 ggcggacgac cggctccagc aggccgagaa ggaggcggcg gaggctcgcg aggctgcaag
     9541 ccgcgacgtc caggagacgc gagatgcggc cgcccgagag gttcaggagc tgacggagag
     9601 agcgcgccgc gaagc
//
LOCUS       DASVXS010000036        20810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1283435, whole genome shotgun sequence.
ACCESSION   DASVXS010000036 DASVXS010000000
VERSION     DASVXS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20810
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283435"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VF752_01955"
     CDS             complement(<1..528)
                     /locus_tag="VF752_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX8854336.1"
                     /translation="MTRLVLRRGTVASVEQTGPVVELTVDVLGERRPAIAYSDLTGPV
                     EVGDDVVVNTAAADLGLGSGGFDLVHVNLTRGLGGVGEAGAHVMKLNYTSLQHAVHPF
                     EEAAPELRGGGGPVAVISLHGQLPCVAWAAAKAVPGARIGFVQTAGGALPGELSEVVA
                     ELRERGMLAAHVTAGP"
     gene            complement(525..1664)
                     /locus_tag="VF752_01960"
     CDS             complement(525..1664)
                     /locus_tag="VF752_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8854337.1"
                     /translation="MEAAAALRATREEKDRLLHDFIRLCEIPSPSGRERRVADVVRAE
                     LEGAGIEVLEDETGAETGSDSGNLLARIPGPHDARTILLGAHLDTVPLAAPVEVSNEE
                     GMLTNRNEAILGADNKTAVAILVAVARRLARAGSPVGVDLLFTTCEEPGLLGARFFDS
                     SQLRAEYGYVFDHASPVGEVIVAAPTYYRIGAHFHGHAAHAGVRPEDGHNAIAAAARA
                     IDRMRFGRLDEGTTANVGTIEGGSARNVVAEHCVVEFEARSLDQARAASLVSEMVDAI
                     TEAASDFECDVETDVAELSRGFRLAPSSQAVTVATRALEGIGVEPVLRPTGGGSDANA
                     FNQQGIPCVNLADGVQHNHEPTERVSVDSLERMLDVALGLVAHSA"
     gene            complement(1669..3366)
                     /locus_tag="VF752_01965"
     CDS             complement(1669..3366)
                     /locus_tag="VF752_01965"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8854338.1"
                     /translation="MPDRRTPTKFIFVTGGVVSALGKGIAAASIGRLLVSRGFKVAIQ
                     KFDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDANTTRQSNVTTGAIYN
                     TVIRRERRGDYLGGTVQVIPHITDEIKNRIRLVADSQDVDFVITEIGGTVGDIESLPF
                     LEALRQFQVDQGRRSCMFVHVTLVPFLGHAGELKTKPTQHSVQELRRIGIQPDMIMCR
                     SEGQLDRDIRQKIALFANLPVESVVSARDVDTIYKVPLYFRAEGVDDQILEHFGVDAP
                     AAEMSEWEAIVKRSDSASDTVKIGLVGKYIQLEDAYLSVIEALKHAGFHHGVRVEVSW
                     IDAEDLDHAEAERQLEECDGILIPGGFGIRGVEGKIRAARVAREREIPYLGICLGMQV
                     AVSEFARHLVGMDGANSTEFDPETPYPVIDLLPEQKEVRDMGGTMRLGADPVKLHDDT
                     RAREIYGEPVIYERHRHRYEVNNHLRKHLEAAGLVCSGTSPDDRLVEMIELPEHPFFV
                     ASQFHPEFKSRPLRPQPLFRDFVGASYERSRGRAPEAEPSVTAGERDRSAERAEQRA"
     gene            complement(3359..4231)
                     /locus_tag="VF752_01970"
     CDS             complement(3359..4231)
                     /locus_tag="VF752_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854339.1"
                     /translation="MQSLPLWVSLVVALAVVPPAVRGLRAQGFLRENYRGALVAFPGG
                     VAAVAAAVVALIPLSLVAELGDADVFTSDAGTVAFYGLGVALLGALDDLIGSGALVPN
                     GATASAPERPRGWRGHARATLVGSFSTGALKAAGALGLALFVLQGRGLSAGEYLLAAA
                     VLVLATNLFNLLDLRPGRSLKALVLLGAGLTIGARDADPLWSVGMFVGPLLVLMPFDL
                     REQAMLGDTGSNLAGAVAGLWIVLALGTLGQAIALGVMAVLTIYGEFRSISQLIERTP
                     LLRSLDSLGRATHA"
     gene            complement(4266..5126)
                     /locus_tag="VF752_01975"
     CDS             complement(4266..5126)
                     /locus_tag="VF752_01975"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX8854340.1"
                     /translation="MFDFRYHALSLVAVFLALGIGIVLGVTIGDSVVSDANKGLRSSL
                     RGDVVAARKAAKDAQANVSERDRWIDSAVPYLIDGRLLGERVALVSTGSLEGDIKKDV
                     DSSVKDAGGTVDSTLDLALPAQTQDLASAMRVTLATDTDEQGLNLLGRRVARAIVRGR
                     GLARVDKALPDRVDGDFTGADAVVLYRSPPPDDATEADNNARQAFENGLIQGLKRQNV
                     PVVGVEATATEPSQIGFFNDRDVSSVDDVDMPGGRAALVLALNGQKGAFGVKGTADAP
                     LPKLQGNGGG"
     gene            complement(5133..6338)
                     /gene="steA"
                     /locus_tag="VF752_01980"
     CDS             complement(5133..6338)
                     /gene="steA"
                     /locus_tag="VF752_01980"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX8854341.1"
                     /translation="MVVPRITRRAPRSGPRSAGTNGRDAAVPSFTATARLGKRTKRLV
                     KRLGPDDIAVIDHRDIDRVSAEDLAATGVKCVVNVAASSSGRYPNAGPLILTEAGIHL
                     VDIPGAPLFDTLKDGEQITVRGGDVLRDGKRIASGEALDLEAVRLAQERGKRQIGDAL
                     ESFAANTIEHMREERDLLVGRVDLPEFSTRFRDRPALVVVRGVDHQKDLRTLRPYVRD
                     MKPVLVAVDGGADAILEQGWKPDMIVGDMDSATDTALRCGAELVVHAYPDGRAPGRER
                     LDKLGLPYKVVPAPATSEDVAMIIAAERGAALIVAVGSHFNLMEFLDKNRKGMSSTFL
                     TRLRIGEILVDAKGVSRLYRPSAGKGPIVVLTLAALATLIVVLVTSPSLGPLLDLLWL
                     KMKIVLGLD"
     gene            complement(6379..6648)
                     /locus_tag="VF752_01985"
     CDS             complement(6379..6648)
                     /locus_tag="VF752_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX8854342.1"
                     /translation="MADRREAVLQRVRATPPGFVRTYGDISPGAPRFGGQVLSETHDP
                     EIPWHRIVRADGSLAKGDRQRRLLEREGVPFAGGRVDMRAARLPD"
     gene            complement(7038..8717)
                     /gene="recN"
                     /locus_tag="VF752_01990"
     CDS             complement(7038..8717)
                     /gene="recN"
                     /locus_tag="VF752_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX8854343.1"
                     /translation="MLLELRIENLLLIERAELRLGAGLNAITGETGAGKTMLAQALDL
                     LLGGKPKSGIVRPGAGEAYVEGVFSVPPGLLDEPALADLRERLPDEDEEIVLSRRVGA
                     DGRTRAFVQGRSASAADLRELGGRLVAFYGQHEHRRLAVGSVQLEILDGFCGDKHLAE
                     RAALADAHLRVTALERELEELRARAGASDRERDLLAYELEEIDELDPSEEEKAGLLGE
                     RERLRRLDALREASGAGAEALAPEAVEGFGVSGLLAQVEQLADGVQGVDPALDALAER
                     LRALRIEADDLGAELRRYESGLEGEPGRLEQVEERLTLYDRLERKHGGTVEAVLEHAR
                     RCRDALERLDDLEGALERIEAELAQARREEDERAGKVSRGRVKAAPKLAERVLAELSE
                     LAMDGASFEVRLQPREVRARTGDERVELLIAPNPGVPAQPLRETASGGEMSRVMLALM
                     SVASTGGAETLVFDEVDAGVGGQTARVVGEKLRALAAGRQVLCITHLPQIASLAEHHF
                     RIEKSAEGDLARTSVEPLADEAVLEELCRMLGADASDAGARKHAEELLAAA"
     gene            complement(8790..9647)
                     /locus_tag="VF752_01995"
     CDS             complement(8790..9647)
                     /locus_tag="VF752_01995"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX8854344.1"
                     /translation="MTRPRTVEIFTHDQPSETDEALKRVVYELVDAGVQVRLPQDEVE
                     KHGFTDGENVVTGGEAGADVDVAVVLGGDGTILQAMRLYAGSGVPVFAFNYGAIGFLA
                     TMERDQLEEGIRNVLESDYDLLRMPALAIEAAGEHHKAVNDISFHRRPNRRVAELAYS
                     VEGEQLGKVRCDGLVVSTPVGSTGYNLANGGPVLAWGVDGFVVSFIAPHTLTARALVV
                     APDAVMTVQNLSSSDPVDVTTDGRIVCPLEPQDEMHVRFDHDQALLAQLPGSSFYHRL
                     RDKFGRLAY"
     gene            complement(9644..10432)
                     /locus_tag="VF752_02000"
     CDS             complement(9644..10432)
                     /locus_tag="VF752_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX8854345.1"
                     /translation="MAKTRLDTLLVERGLFESRSRAAAAVIAGDVRLGDERRRADKPG
                     QMVGGDVPVEVIGGPPFVSRGGVKLANALETLGIEPSGRRVLDVGASTGGFTDCLLQH
                     GAASVIALDVGYGELHWRLRNDPRVTTMERTNARDLDCAALPYRPDLVVADVSFISLE
                     KVLPAVLSCAEERFDCLALVKPQFEVGRERLGKGGVVRDASDRRDALVSVGRFAVDQG
                     WTLLGFASSGLPGPAGNRETFAWIVEGGRGGGVDPEAAALEVEP"
     gene            10508..10867
                     /locus_tag="VF752_02005"
     CDS             10508..10867
                     /locus_tag="VF752_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854346.1"
                     /translation="MTHTGHMPIQMHAAAEFAFGVLLVAAAFALGLGATAIGIAILAG
                     ILLVTLALGATEPGGRGSIPVSAHATYDWAIGFGMVAAGIVFAFAGNLGAFGLFAVGG
                     AIELLLASRTRYSAAAR"
     gene            complement(10964..11707)
                     /locus_tag="VF752_02010"
     CDS             complement(10964..11707)
                     /locus_tag="VF752_02010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854347.1"
                     /translation="MDPRAAQGFSASADSYQEARPSWPPEAIDAAFAHWGLDPASAHV
                     VDVAAGTGRLTAVLAARCPHVIAVEPLAEMRAHITAAEALEGTAEQLPLEDASADAIF
                     VAEAFHWFDQPAALAEFARVLKPRGGLALLWNEHVTTAEDEEAMASAFGGMFGPGGQF
                     QPPDKSGWDEGPQWAPFEPIVKREFEHTQHLTRDGLVKLVASFSFIGALQPEARREVL
                     SRVEKGLAEEGLDEHEQRWRCELYLTRLR"
     gene            complement(11710..13662)
                     /gene="dxs"
                     /locus_tag="VF752_02015"
     CDS             complement(11710..13662)
                     /gene="dxs"
                     /locus_tag="VF752_02015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX8854348.1"
                     /translation="MSLLEKIDGPNDLHGLNDEQLAQVAQEVRQLIIDTIGEIGGHFG
                     ANLGTCELAVALHSLLDSPRDKILWDVGHQAYPHKVLTGRRDRLDTIRKYGGLAPFCS
                     IAESEHDIMGAGHASTSVSYAVGVKEAMLRGAAPDGKVLAVIGDGALTGGVAFEALHN
                     AGGMGLPIVIVLNDNGMSIAPNVGALSKYFNRVRLNPKLHKAREDVEEGLTKLPAGIG
                     ERIERLGPQLKEAIKAFWAPGLLFEELDLAYVGVIDGHDVGQLREAIGKALKADRPVV
                     VHIKTVKGKGFAPAEEGGLEGMEKWHAAKPASIVNGQPAPKPKQNGSKRFAPTPPPQY
                     TNVFGDAMVEECNRDSRVCGITAAMNSGTGLNILQKAMPDRYYDVGIAEQHAVLLAAG
                     MALQGMKPVAAIYSTFLQRAFDQIVHDVCLQKLNVVFAMDRAGLVGDDGPTHHGAFDI
                     AYQRCLPNIVLMAPRDEALLKQMLRTALLHDDGPVAIRYPRGEAVGVEMPAEIEPIAI
                     GRGELLREGERVAFLGYGDGVNIALGAADQLAEHSVNATVADARFAKPLDEELIEQLV
                     SEHEVLVTVEDGCLKGGFGSAVGEVLLDRQLAGEARVMRFGLPDRYVTHGKPALLRQE
                     IGLTPEAIAEQVADVVLDPRGALQSA"
     gene            complement(13659..14549)
                     /locus_tag="VF752_02020"
     CDS             complement(13659..14549)
                     /locus_tag="VF752_02020"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX8854349.1"
                     /translation="MSTAAPAAGYPTHLQSEVDAYLSELGFGDEPATAGLSEAMRYSL
                     LAGGKRIRPVLALATAEAVGRPSGDVMPLAAAIELIHTYSLIHDDLPAMDDDELRRGQ
                     PTCHVVYGEDVAILAGDGLFAEAVKLVLERQQGEPANLLAAVHELVSAAAVSGMVGGQ
                     YVDVTAPGDLDEAGLRHLHELKTGRLIAASVQSVLALTGAPEPATIAYRRFAAELGVL
                     FQIVDDILDVTGDEAALGKPQGSDQRHGKRTYVSVHGLDRARELARESHAKARAALAD
                     AGGRTQTLEQITDYILTRQT"
     gene            complement(14546..14764)
                     /locus_tag="VF752_02025"
     CDS             complement(14546..14764)
                     /locus_tag="VF752_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854350.1"
                     /translation="MTAEAGQDSLSRLVQELEQAASQLRSGELEGEQAAALVERCADL
                     AGEVAGELERRDRAVASGESVPGQERLL"
     gene            complement(14761..15144)
                     /gene="nusB"
                     /locus_tag="VF752_02030"
     CDS             complement(14761..15144)
                     /gene="nusB"
                     /locus_tag="VF752_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX8854351.1"
                     /translation="MRRTEQRRAAVVALYQHDVTGRPLDEIVDRNAKPFTRELVECTA
                     AVQPRLDELIERHSVGWTLDRIAPLERNILRVALYELLDRDDVPDEVAIDEAVEAAKE
                     LCGAEAPAFINGILGSVQREAVASQ"
     gene            complement(15154..15714)
                     /gene="efp"
                     /locus_tag="VF752_02035"
     CDS             complement(15154..15714)
                     /gene="efp"
                     /locus_tag="VF752_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX8854352.1"
                     /translation="MISTNQFKNGSHIDVDGTVFKIVEFQHVKPGKGGAFVRTKLKRT
                     ADGAVIDKTFRAGEKFRQVRTESRKMQFLYTDGTDAHLMDMETYEQSTVPEGQVRDAL
                     KWVLPSQEVDVLYIDGNAADLQVPSAVELSVSETEPGLRGDTASGGGDKPATLETGAA
                     VRVPLFVNVGDRVRVDTRSGEYVARA"
     gene            complement(15736..16188)
                     /locus_tag="VF752_02040"
     CDS             complement(15736..16188)
                     /locus_tag="VF752_02040"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX8854353.1"
                     /translation="MSDHYQRPGWFTKNVFNRTVAALTRVGISVAGSRVLEVKGRKTG
                     EPRRTPVNLLTYEGGRYLVAPRGHTQWVKNLRASPQGRLLVGPRSESFTATELPDEQK
                     TELLRAYLRKWKWEVGAFFGGVDADSSDADLQRIAPDHPVFRVESRAS"
     gene            complement(16202..17146)
                     /locus_tag="VF752_02045"
     CDS             complement(16202..17146)
                     /locus_tag="VF752_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8854354.1"
                     /translation="MPNEIDIEHLYGAQLKGRSADLLLAQLAARQHGVVDRDELLGIG
                     LGRGAIRKRVDSGRLHLVYRGVYAVGHPKLTREGEWMAAVRAGRPDAHLSHRSAASLY
                     CLLKFQPATHEVTAPRQKQSRGPLKFYWRELPEDEVTTHDGIPATTIPRTIFDLAAVA
                     PKRHVERAIDEAEVRRLTDKLSLPDLLERYPGARGTAVIRAILEAGQLGATITRGDLE
                     ELFIAFCDEHGLPRPQMNVPIQLPDRWIEADCVWHDKRVIVELDDRMSHDRTAAFEKD
                     RSRDRMLSVNGWTVIRITAQQLMYDAREIAADLRRLLH"
     gene            complement(17199..17627)
                     /locus_tag="VF752_02050"
     CDS             complement(17199..17627)
                     /locus_tag="VF752_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8854355.1"
                     /translation="MAEEPRERALRFWEDVFNAHEIDSIGDWVSDDFVNHNALEGTPR
                     GPEGARMAFERMWTAFPDMRFEVEDVIADGNKVACVGHMTGTHSGEFEGMKPTNNGFR
                     ARHAHILTSGDEGKWTEHLAIRDDVEMLRQLGVMPGPPGR"
     gene            complement(17630..18727)
                     /locus_tag="VF752_02055"
     CDS             complement(17630..18727)
                     /locus_tag="VF752_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX8854356.1"
                     /translation="MAGRIVLFGATGYTGRLVAHALEKRGARPVLAARSREKLEQLAS
                     ERGGLETQVADVSDPKSVRALVERGDVLVSTVGPFVRWGRPAVEAAVDAGAHYIDSTG
                     EPPFIRAVFEEYGAAADKAGCGLLTAFGYDYVPGNLAGALALERAGEHAEKVSVGYFF
                     TGRGSIKGAASGGTLASMGGVMLEPHFAFREGRVVTERPAKRVRSFDRNGRAMTGISI
                     GSSEHFGLPRVHPSLREVDAYLGWFGPASRAVQAVSVTGDIPGMRSLANGLVTRFVKG
                     STGGPDAEARSKTGSWFPAEAADSGGNVLARAELSGPNGYTFTGDIMAWGAQRAASEG
                     LEGTGALGPVEAFGLRVLEAGSADVGLKEAG"
     gene            18765..19184
                     /locus_tag="VF752_02060"
     CDS             18765..19184
                     /locus_tag="VF752_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8854357.1"
                     /translation="MTVTESLRETRDRIVREHMESENRHEFDVTLATFDHPRYEIVPT
                     GDVYDGPQEVSAYFTESRTAFPDQRNELIALHHADDAVIAEFWLKGTHRGPLRGIPPT
                     GREFSCRCVAIFVFEDDRLVCERAYFDAATILRQLTG"
     gene            complement(19174..19923)
                     /locus_tag="VF752_02065"
     CDS             complement(19174..19923)
                     /locus_tag="VF752_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8854358.1"
                     /translation="MSEVERVTEDVVRVRASNASAFTLGGTNSYIVRDWVVDPGPRDS
                     SHVEALLEAAGGRIDGIAITHRHLDHYGGAAELAERAGGVPVVWPVDGDDVGPFRAIA
                     TPGHSADHVALVYRKICFTGDAVLGLGSVFISAEGGGLTAYMYALRLLRSLDLELLCP
                     GHGPVVRDPAAKLDEYIAHRMEREEKLIAALDGGARSVDELLAQAWDDVPPQLMEHVR
                     PAAEATLAAHLEKLASEGRLPEGVERPGVSL"
     gene            19986..>20810
                     /locus_tag="VF752_02070"
     CDS             19986..>20810
                     /locus_tag="VF752_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8854359.1"
                     /translation="MAPDELMWKSAAEQAELVRSGEVSSRELVKASLEAIDRHNGELN
                     AVVTLMTEEALEQADAVQPGDDRPLAGVPMLVKDLIALTKGVRTTFGMRGMGEWAPSV
                     DSSTVRRLRDAGAIIVGKTNTPEMGILPVTEPLRFGPARNPWDTSRTPGGSSGGSGAA
                     VGSGMVAIAHGNDGGGSLRIPGSCNGLVGLKPSRGRISWAPEFEELVAGFAIDGVLSR
                     TVLDTAIGLDVLSGYETGDPYWAPDPSAPFVEAVGREPGRLRVLWTAHAPNGAPVDD"
BASE COUNT         3070 a         7503 c         7016 g         3221 t
ORIGIN      
        1 aggtccggcg gtgacgtgcg cggcgagcat gccacgctcg cgcagttcgg cgaccacctc
       61 tgagagctca cccggcagcg cgccgcctgc ggtctgcaca aaaccgatcc gcgcgcccgg
      121 cactgccttc gccgccgccc acgccacgca cggcagctga ccgtgcagcg agatcaccgc
      181 caccggccca cctccgccgc ggagctcggg agccgcctcc tcgaagggat gcacggcgtg
      241 ctggagcgag gtgtagttca gcttcatcac gtgcgcgccg gcctctccta cgccgccgag
      301 gccgcgcgtg aggttcacgt gcacgaggtc gaagccgccg ctgcctagcc cgagatcggc
      361 tgcggcggta ttcacgacca cgtcatcccc gacctctact ggccccgtga ggtccgagta
      421 ggcgatcgcc gggcggcgct cgccgagcac gtccaccgtc agctcgacga cgggtcccgt
      481 ctgctcaacc gaggcgacgg tgccgcgtcg caggacgagc cgggtcacgc gctgtgggcc
      541 accaggccga gcgcgacgtc gagcatgcgc tcgagcgaat cgaccgacac ccgctcggtc
      601 ggttcgtggt tgtgctgtac gccgtcggcg aggttcacgc acgggatgcc ctgctggttg
      661 aaggcgttcg cgtcgctgcc gccgccggtg ggccgcaaca cgggctcgac gccgatcccc
      721 tcgagcgccc gggtcgcgac cgtcacggcc tgagagctcg gcgccagccg gaacccgcgc
      781 gagagctcgg cgacgtccgt ctcgacgtcg cattcgaagt cgcttgcggc ctcggtgatc
      841 gcgtcgacca tctcggacac gagcgaagcc gcgcgcgcct gatcgaggct tcgcgcctcg
      901 aactcgacca cgcagtgctc ggcgaccacg ttgcgcgcgc tgccgccctc gatcgttccg
      961 acgttcgcgg tcgtgccctc gtcgaggcgc ccgaagcgca tgcggtcgat cgcccgagcc
     1021 gccgccgcga tggcgttgtg cccgtcctcc ggccgcacgc cagcgtgcgc ggcgtggccg
     1081 tggaagtggg cgccgatccg gtagtaggtg ggcgcagcaa cgatcacctc tcccactgga
     1141 gaggcgtgat cgaacacgta gccgtactcg gctcgaagct gcgagctgtc gaagaacctg
     1201 gcgccgagca gtccgggctc ctcgcacgtg gtgaagagca gatccacgcc gaccggcgaa
     1261 ccggcgcgtg cgagccggcg cgcgaccgcc acgaggatcg ccaccgccgt cttgttgtcg
     1321 gccccgagga tcgcctcgtt gcgattcgta agcatgccct cctcgttcga cacctcgacc
     1381 ggcgcggcga gcggcaccgt gtcaaggtgg gcgccgagca ggatcgtgcg cgcgtcgtgt
     1441 ggtccgggga tgcgcgcgag cagattgcca gagtcggagc cggtctcagc accggtctcg
     1501 tcctcgagca cttctatgcc tgcgccctcg agctcggcgc gaaccacgtc cgccacccgc
     1561 cgctcgcggc cggacgggct cggaatctcg cagaggcgga tgaagtcgtg gagcaggcgg
     1621 tccttctcct cgcgcgtagc gcgcaatgcg gccgccgcct ccatgggact acgcgcgctg
     1681 ttcggcgcgc tccgcggagc gatcgcgctc gcccgcggtc acgctcggct cggcctccgg
     1741 cgcgcggccg cgcgagcgct cgtacgacgc gccgacgaag tcgcggaaga gcggctgcgg
     1801 gcgcagcgga cgcgacttga actccggatg gaactgcgat gccacgaaga acgggtgctc
     1861 cggcagctcg atcatctcga cgagccggtc gtcgggcgac gtgcccgagc agacgaggcc
     1921 ggcggcttcc aggtgcttgc gcaggtggtt gttgacctcg tagcggtggc ggtggcgttc
     1981 gtagatcacg ggctcgccgt agatctcgcg ggcacgcgtg tcgtcgtgca gcttcaccgg
     2041 gtcggcgccg agccgcatgg tgccgcccat gtcgcgcact tccttctgct cgggcagcag
     2101 gtcgatcacg gggtaagggg tctcggggtc gaactcggtg gagttcgcgc cgtccatgcc
     2161 caccaggtgc cgcgcgaatt ccgacacggc cacctgcatg ccgaggcaga tgccgaggta
     2221 cgggatctcg cgctcgcgcg cgacccgcgc cgcgcggatc ttgccctcca cgccgcggat
     2281 gccgaagccg cccgggatga ggatgccgtc gcactcctcg agctgccgct ccgcctcggc
     2341 gtggtcgagg tcctcggcgt cgatccagct gacctccacg cgcacgccgt ggtggaaccc
     2401 ggcgtgcttg agcgcctcga tcaccgacag gtaggcgtcc tcgagctgga tgtacttgcc
     2461 cacgagcccg atcttcacgg tgtcgctggc cgagtcgctc cgcttgacga tcgcctccca
     2521 ctccgacatc tccgccgccg gcgcgtcgac accgaagtgc tccaggatct ggtcgtcgac
     2581 tccctccgcg cggaagtaga gcggcacctt gtagatcgtg tcgacgtcgc gcgccgagac
     2641 gaccgactcg accggcaggt tcgcgaacag cgcgatcttc tgccggatgt cgcggtcgag
     2701 ctggccctcg gagcggcaca tgatcatgtc cggctggata ccgatgcggc ggagctcctg
     2761 caccgagtgc tgcgtgggct tcgtcttcag ctcgcccgcg tggccgagga acggcacgag
     2821 cgtgacgtgc acgaacatgc acgagcgacg cccctggtcg acctggaact ggcgcagcgc
     2881 ctcgaggaac ggcagcgact cgatgtcgcc caccgtgccg ccgatctccg tgatcacgaa
     2941 gtcgacgtcc tgcgaatcgg cgacgaggcg gatgcggttc ttgatctcgt ccgtgatgtg
     3001 cgggatcacc tggacggtgc cgccgaggta gtcgccgcgg cgttcgcggc ggatgaccgt
     3061 gttgtagatc gccccggtcg tgacgttcga ctggcgcgtg gtgttcgcgt cggtgaagcg
     3121 ctcgtagtgg ccgaggtcga ggtctgtctc ggcgccgtcc tcggtcacga acacctcgcc
     3181 gtgctggaac gggctcatcg tgccgggatc gacgttgatg tacgggtcga acttctgaat
     3241 cgcgaccttg aagccgcgcg acaccagaag tcggccgatc gatgcggccg cgatcccctt
     3301 gccgagagcg gagaccacgc ccccggtcac gaagatgaac ttggtcggcg tgcgtcgatc
     3361 aggcatgcgt tgctctcccc agcgagtcta gtgatctaag cagcggagtc ctttcgatca
     3421 actgcgagat cgagcggaac tccccgtaga tggtcaaaac ggccattacg ccgagcgcga
     3481 tcgcctggcc gagcgtcccg agcgcaagca cgatccagag cccagcgacg gctcccgcga
     3541 ggttcgagcc ggtgtcgccg agcatcgcct gctcgcgcag gtcgaacggc atcagcacaa
     3601 gcagcggacc gacgaacatg cccacgctcc agagagggtc ggcgtcccgc gccccgatgg
     3661 tgagcccggc cccgagcagg acgagcgcct tgagcgaccg cccgggtcgc aggtccagca
     3721 ggttgaacag gttcgtagcg agcacgagca ccgccgccgc gagcaggtac tcccccgcgc
     3781 tcaggccgcg gccttggagc acgaagagcg caaggccgag cgcccccgcg gccttcaagg
     3841 caccggtcga gaagctgccg acgagcgtgg cgcgcgcgtg accgcgccag ccccgcgggc
     3901 gctcgggagc cgaggcggtc gcgccgttcg gcacgagcgc gccgctgccg atcaggtcgt
     3961 cgagcgcccc gaggagcgcg accccgagcc cgtagaaggc gaccgtgccg gcgtcgctcg
     4021 tgaacacgtc cgcgtcgccg agctccgcga cgagcgagag cgggatcagc gcgacaacag
     4081 ctgcggcgac ggccgccacc ccacccggaa aggcgaccag cgcgcccctg tagttctccc
     4141 gcagaaagcc ctgtgcgcgc agcccccgga ccgccggcgg caccactgcg agggcgacga
     4201 ccagcgagac ccagagcggg agagattgca cctcaggaac ctacagccgg tggcggacgg
     4261 cggtgctatc cgccgccgtt cccctggagc ttcggaagcg gcgcgtccgc tgtgcccttc
     4321 acgccgaagg cacccttctg gccgttcagc gcgagcacga gcgctgcgcg ccctcctggc
     4381 atgtccacgt cgtcgacact cgagacatcg cggtcgttga agaacccgat ctgcgatggc
     4441 tcggtggcgg tggcctcgac cccgacgacc ggaacgttct gccgcttcag cccctggatc
     4501 agcccgttct cgaatgcctg cctggcgttg ttgtccgcct ccgtggcgtc gtcgggcggc
     4561 ggcgagcggt agaggaccac ggcgtcggct ccagtgaagt cgccgtcaac acgatcgggg
     4621 agcgccttgt cgacgcgagc gagcccgcgg ccgcgcacga tcgcgcgggc cacccgccgc
     4681 ccgagcaggt tgaggccctg ctcgtcggtg tcggtggcga gcgtcacgcg catggcgctc
     4741 gccagatcct gcgtctgcgc gggcagcgcg aggtccaagg tcgagtcgac cgtgccgccc
     4801 gcgtccttca ccgacgaatc gacgtctttc ttgatgtcgc cctcgagcga acccgtggac
     4861 accagcgcca cccgctcacc gagcagccgc ccgtcgatca ggtatggaac cgccgagtcg
     4921 atccagcggt cgcgctcact cacgttggcc tgcgcgtcct tggcagcttt gcgagcggca
     4981 acgacatcgc cccgcagcga cgagcgcagg cccttgttcg cgtcggacac caccgaatcg
     5041 ccgatcgtca ccccgagcac gatcccgatg ccaagggcga gaaagaccgc cacgagcgag
     5101 agcgcgtggt agcggaagtc gaacacgggt ctctagtcca atcccaggac gatcttcatc
     5161 ttcagccaca gcagatcgag cagcgggccg aggctgggcg aggtgacgag gaccacgatc
     5221 agcgtggcca gcgccgccaa ggtgaggacg acgataggac ccttgcccgc cgaagggcgg
     5281 tagagccggc tcacgccctt cgcgtccacg aggatctcgc cgatccgcag gcgcgtgagg
     5341 aaggtcgacg acatgccctt gcggttcttg tcgaggaact ccatcaggtt gaagtgggag
     5401 ccgacggcga cgatcagcgc cgcgccgcgc tcggccgcga tgatcatcgc gacgtcctcg
     5461 ctcgtggccg gcgccggcac gaccttgtac ggcagcccga gcttgtcgag gcgctcgcgg
     5521 cccggcgcgc gcccgtcggg gtaggcgtgg acgaccagct cggcgccgca gcgcagcgcg
     5581 gtgtccgtgg cggagtccat gtcgccgacg atcatgtccg gcttccagcc ctgctcgagg
     5641 atggcgtccg cgccgccgtc gaccgccacg agcaccggct tcatgtcgcg cacgtagggc
     5701 cgaagcgtcc gcaggtcctt ctgatggtcg accccgcgca ccacgacgag cgccggccgg
     5761 tcgcggaagc gcgtgctgaa ctcgggcagg tcgacccggc ccacgagcag gtcgcgctcc
     5821 tcgcgcatgt gctcgatcgt gttcgcggcg aacgactcga gagcgtcgcc gatttgccgc
     5881 tttccgcgct cctgtgcgag ccggaccgcc tcgaggtcga gcgcttcgcc actcgcgatc
     5941 cgcttgccgt cgcgcagcac gtcgccgccg cgcacggtga tctgctcgcc gtccttgagc
     6001 gtgtcgaaga ggggcgcgcc cggaatgtcg acgaggtgga tgccggcctc ggtgaggatc
     6061 agcgggcccg cgttcggata gcggccgctc gacgacgccg ccacgttcac gacgcacttc
     6121 acgccggtcg cggcgagatc ctcggccgag acgcggtcga tgtcgcggtg atcgatgacc
     6181 gcgatgtcgt ccgggcccaa gcgcttaacg aggcgcttgg tgcgcttgcc cagccgggcc
     6241 gtggccgtga acgagggaac ggccgcgtca cgcccgttag tgccagcgct gcgcggcccc
     6301 gaacgcggcg ctctgcgtgt gatccgcgga accaccatcg gatctgtgaa ctgtacgcgc
     6361 gctggtggaa gcgcgcgctc agtcaggcag ccgggcggcc ctcatgtcga cacgcccgcc
     6421 cgcgaacggc acgccctccc gttcgagcag acgccgctgc cgatcgccct tcgcaagcga
     6481 accgtcggcc cgcacgatgc ggtgccaggg gatttcgggg tcgtgcgtct cggacagcac
     6541 ctgcccgccg aatcgtggcg cgcctggcga gatgtcgccg taggtgcgca cgaagccggg
     6601 cggtgtggcg cggacgcgct ggagcaccgc ttctcgcctg tctgccatgg cggtgaatgt
     6661 acgggacgcg gacacagcgg caatgagggt tgggctcgca ggagttgccg gtgcctacca
     6721 atcgacctcc gtggcgcgga aatcgtcgaa gggtcctgga ttacccccgt ctatgggcgg
     6781 taatccagga cggtagggcg aaaaacgccc cgccgagctt cgtaagtgct ttgctgttcg
     6841 cccgtgaatt ggggacggca gcggtgaagg tctctcgacc tgactttctt gccgcatacc
     6901 ggtaagaaac tcaggagttg ggctcgccgg ggcgtaagtc ctgactttgg ttctccatat
     6961 cggaaccaac ctcaggagtt gcggggcggc cgcgccgagc tcacgggtga tcatccagat
     7021 gcacggtcgt cgggcggcta tgccgcggcg agcagctctt cggcgtgctt gcgcgctccc
     7081 gcgtccgatg cgtccgcgcc gagcattcgg cacagctctt cgagcacggc ctcgtccgcg
     7141 agcggctcta cggacgtccg agccagatcg ccctccgcgc tcttctcgat ccggaagtga
     7201 tgctccgcca gcgaggcgat ctgcggcagg tgcgtgatgc acaggacctg gcgcccggcg
     7261 gcgagcgccc gcagcttctc ccccaccacg cgcgcggtct ggccgccgac gcccgcgtcg
     7321 acctcgtcga acacgagcgt ctccgcgccg cccgtgctcg ccaccgacat cagcgcgagc
     7381 atcacgcgcg acatctcgcc gcccgaggcg gtctcgcgca gcggctgcgc cggcacgcca
     7441 ggattcggcg ctatcagaag ctcaacccgc tcgtcgcccg tgcgggcgcg gacctcgcgt
     7501 ggctggagcc tcacctcgaa cgacgcaccg tccatcgcca gctcggacag ttcggcgagt
     7561 acgcgctccg cgagcttcgg cgccgccttc acgcgcccgc gcgacacctt cccggcgcgc
     7621 tcatcctcct cgcgccgcgc ctgcgccagc tcagcctcga ttcgctcgag cgccccctcc
     7681 aagtcgtcga gccgctcgag cgcatcgcga catcggcgcg catgctccaa cacggcctcg
     7741 accgtgccgc cgtgctttcg ctcgagccgg tcgtagagcg tgaggcgctc ctccacctgc
     7801 tcgagccgcc ccggctcgcc ctcgagcccc gactcgtagc ggcgtagctc ggcgccgagg
     7861 tcgtcggcct cgatccgcag cgctcgcagg cgctccgcaa gcgcatcgag cgccgggtcc
     7921 acgccctgca cgccgtccgc gagctgctcc acctgcgcca ggagcccgct cacgccgaag
     7981 ccctcaacag cttccggggc gagcgcctca gcgccggcgc ccgacgcctc acgcagcgcg
     8041 tcgagacgcc ggaggcgctc gcgttccccg agcaggccgg ccttctcctc ttcgctcggg
     8101 tcgagttcgt cgatctcctc gagctcgtag gccagcaggt cgcgctcgcg gtcggacgcg
     8161 ccggcccgtg cccgcagctc ctcgagctcg cgctcgagcg cagtcacgcg caagtgcgcg
     8221 tcggcgagcg ccgcgcgctc agccaggtgc ttgtcgccgc agaagccgtc gaggatctcg
     8281 agctgcaccg agcccaccgc caggcggcgg tgctcgtgct ggccgtagaa ggccaccaac
     8341 cgcccgccga gctcgcgcag gtcggccgcg ctcgccgagc gcccctgcac gaatgcgcgt
     8401 gtgcgcccgt cggccccgac gcggcgactg agcacgatct cctcgtcctc gtccggcaat
     8461 cgctcgcgta gatcggccag cgccggctcg tcgagcaacc ccggcggcac cgagaacacg
     8521 ccctccacgt acgcctcgcc ggcaccgggc ctcactatgc cgctcttcgg cttgccgcca
     8581 agcagcaggt cgagcgcctg ggcgagcatc gtcttgcccg cgcctgtctc gcccgtgatc
     8641 gcgttcaggc ccgctccgag ccggagctcg gccctttcga tcagcagcag gttctcgatc
     8701 cgcagctcga ggagcacaag ccgaggtgta ccaacggcgc ccgacggagc cgcgacgcgc
     8761 gtaacgctgc ggtcacagag atttgcggtt cagtaggcca agcggccgaa cttgtcgcga
     8821 agccggtggt agaagctcga ccccggcagc tgcgccagga gcgcctggtc gtggtcgaag
     8881 cgcacgtgca tctcgtcctg cggctcgagc gggcacacga tccgcccgtc cgtggtcacg
     8941 tccaccggat cggagctcga gaggttctgc acggtcatga ccgcgtcagg ggcgaccaca
     9001 agcgcgcggg ccgtgagcgt gtggggcgcg atgaacgaga ccacgaaccc gtccactccc
     9061 caggcgagca cgggtccgcc gttggcgagg ttgtagccgg tcgagccgac cggcgtggag
     9121 acgaccagac cgtcgcaacg gaccttgccg agctgctctc cctcgaccga gtaggcgagc
     9181 tcggcaaccc gcctgttcgg gcgccggtgg aacgagatgt cgttgaccgc cttgtggtgc
     9241 tcacccgcgg cctcgatcgc gagcgccggc atgcgcagca ggtcgtaatc gctctcgagc
     9301 acgttccgga tgccctcctc gagctggtcg cgctccatcg tcgcgaggaa gccgatcgcg
     9361 ccgtagttga acgcgaacac cggcacgccg ctgcccgcgt agaggcgcat cgcctggagg
     9421 atcgtgccgt cgccgccgag cactaccgcg acgtcgacgt ccgcccccgc ctcgccgccc
     9481 gtcaccacgt tctcgccgtc ggtgaagccg tgcttctcga cctcgtcctg cggcaggcgc
     9541 acctgcacac ccgcgtccac gagctcgtac acgacgcgct tgagcgcctc gtcggtctca
     9601 gacggctggt cgtgcgtgaa gatctcgacg gtgcggggcc tggtcatggc tccacctcga
     9661 gcgccgccgc ctcgggatcg acgcccccgc cgcgaccgcc ctcgacgatc cacgcgaacg
     9721 tctcgcggtt gcccgcgggg ccgggcagcc ctgaggaggc gaagccgagc agcgtccatc
     9781 cctggtcgac cgcgaagcgt cccaccgaca cgagcgcgtc acggcggtcc gacgcgtcgc
     9841 gcaccacgcc gcccttgccc aagcgctcgc gcccgacctc gaattgcggc ttcacgagcg
     9901 cgaggcagtc gaagcgctct tcggcgcacg acagcacggc gggcagcacc ttctcgagcg
     9961 agataaacga tacgtccgcc acgacgaggt cgggacggta cggcaacgcg gcgcaatcga
    10021 ggtcgcgcgc gttggtgcgt tccatcgtcg tgactcgggg atcgttgcgc agccgccagt
    10081 gcagctcccc gtagcccacg tcgagtgcga tcacggacgc ggcgccgtgc tggagcaggc
    10141 agtcggtgaa gccgcccgtc gaagcgccga catccagcac acgtctcccg gacggctcga
    10201 tacccaaggt ttccagggca ttcgcgagct tcacaccccc gcgcgagacg aatggcgggc
    10261 caccgatcac ctccaccggc acgtcgcccc cgaccatctg accgggctta tcggcccgac
    10321 gccgctcgtc gcccagcctc acgtcgccgg cgatcacggc cgcggcggcc cgcgagcggc
    10381 tctcgaacag cccgcgctcc acgagcaggg tgtccaggcg ggtcttcgcc acgggccgga
    10441 gcgtatacgg ggcgggcgtg acacccgtca ccgacaagct ggatacgcag gcgtacattt
    10501 cttgtcgatg acgcacacag ggcacatgcc gattcagatg cacgccgccg ctgagttcgc
    10561 cttcggcgtg ctcctcgtgg ccgccgcctt cgctctcgga ctgggcgcga ccgccatcgg
    10621 gatcgcgatc ctggccggca tcctgctcgt cacgctggcg ctcggcgcca ccgagccggg
    10681 aggccgcgga tcgatccccg tgagcgcgca cgcgacctac gactgggcga tcggcttcgg
    10741 gatggttgca gccgggatcg tcttcgcgtt cgccggcaac ctcggcgcct tcggcctctt
    10801 cgccgtcggc ggggcgatcg agctcctgct tgcgtcacgc acccgctact ccgccgcagc
    10861 gcggtagcgc gggcaaaaac ttccctcatc gatcaaaaac acacgcatca ctcctccctc
    10921 gagcggcccg ccaccccggc gggtcgctca cttttccagg aggtcagcgc aggcgggtga
    10981 ggtagagctc gcagcgccag cgctgctcgt gctcatccag gccctcctcc gcgaggccct
    11041 tctcgacgcg tgagagaacc tcccggcgcg cctccggctg cagtgcgccg atgaaggaga
    11101 acgacgcgac gagcttcacc agcccatcgc gggtcagatg ctgcgtatgc tcgaactcgc
    11161 gcttgacgat cggctcgaac ggcgcccact gaggcccctc gtcccagccg ctcttgtcgg
    11221 gcggctgaaa ctgcccgccg gggccgaaca tgccgccgaa ggcgctggcc atcgcttcct
    11281 cgtcctcggc agtggtcacg tgctcgttcc acaggagcgc gagcccgccg cgcggcttca
    11341 gcacgcgtgc gaactcggcg agcgccgcgg gctgatcgaa ccagtggaac gcctcggcca
    11401 cgaagatcgc gtcggcgctc gcgtcctcga gcggcagctg ctcggcggtg ccttcgagcg
    11461 cctccgccgc cgtgatgtgc gcgcgcatct cggcgagtgg ctcgacggcg atcacgtgcg
    11521 ggcagcgcgc cgccaggacg gcggtgagcc ggccggtgcc tgccgcgacg tcgaccacat
    11581 gcgcgctcgc ggggtcgagg ccccagtgag cgaaggcggc gtcaatcgcc tcgggcggcc
    11641 acgacgggcg cgcctcctga tacgagtccg ccgaggcgct gaagccctgg gcggcccttg
    11701 gatccatgcc tatgcgctct gcagcgcgcc gcgcggatcg agcacgacgt cggccacctg
    11761 ctcggcgatc gcctccggcg tgagcccgat ctcctggcgc agaagggccg gcttgccgtg
    11821 cgtgacgtac cggtcgggca ggccgaaccg catgacccgc gcctcgcctg ccagctggcg
    11881 gtcgagcagc acctcaccca cggccgagcc gaagccgccc ttcaggcagc cgtcctcgac
    11941 cgtcacgagc acctcgtgct cggacacgag ctgctcgatc agctcctcgt cgagcggctt
    12001 cgcgaagcgg gcgtcggcca ccgtggcgtt cacgctgtgc tcggcaagct ggtcggcggc
    12061 gccgagcgcg atgttcacgc cgtcgccgta gccgaggaac gccacgcgct cgccctcgcg
    12121 aagcagctcg ccgcggccga tcgcgatcgg ctcgatctcg gctggcatct cgaccccaac
    12181 ggcctcaccg cgcgggtagc ggatcgcgac cgggccgtca tcgtggagca gcgcggtgcg
    12241 cagcatctgc ttgagcagcg cctcgtcgcg cggcgccatc agcacgatgt tcggcaggca
    12301 gcgctggtag gcgatgtcga aggccccgtg gtgcgtgggt ccgtcatcgc cgacgagccc
    12361 ggcgcggtcc atcgcgaaga ccacgttcag cttctgcagg cacacgtcgt gcacgatctg
    12421 gtcgaacgcg cgctggagga aggtcgagta gatcgccgcg accggcttca tgccctgcag
    12481 cgccatgcca gcggcgagga gcaccgcgtg ctgctcggcg atgcccacgt cgtagtagcg
    12541 gtccggcatc gccttctgga ggatgttgag cccggtgccg gagttcatcg cggcggtgat
    12601 cccgcacacg cgggagtcgc ggttgcactc ctccaccatc gcgtcgccga agacgttcgt
    12661 gtactgcggc ggcggcgtcg gcgcgaagcg cttcgagccg ttctgcttcg gcttgggcgc
    12721 gggctggccg ttcacgatcg aggcgggctt ggcggcgtgc cacttctcca tgccctcgag
    12781 gccgccctcc tcggcgggcg cgaagccctt ccccttgacg gtcttgatgt ggactaccac
    12841 cgggcggtcg gccttcagcg ccttgccgat cgcctcgcgc agctgcccga cgtcgtggcc
    12901 gtcgatcacg cccacgtagg ccaggtcgag ttcctcgaac agcaggccgg gcgcccagaa
    12961 ggccttgatc gcctccttga gctgagggcc gaggcgctcg atgcgctcgc cgatcccggc
    13021 gggcagcttc gtgagcccct cttcgacgtc ctcacgcgcc ttgtgaagct tcggattgag
    13081 gcgaacgcgg ttgaagtact tcgacaacgc gccgacgttc ggggcgatcg acatgccgtt
    13141 gtcgttcagc acgatcacga tcgggaggcc catgcctccc gcgttgtgca gcgcctcgaa
    13201 cgcgacgccg ccggtgagcg cgccgtcgcc gatcaccgcg aggaccttgc cgtccggggc
    13261 ggcgccccgc agcatcgcct ccttgacgcc aaccgcgtag gagacagagg tggaggcgtg
    13321 cccggcgccc atgatgtcgt gctccgactc ggcgatggag cagaacgggg cgaggccgcc
    13381 gtacttgcgg atcgtgtcga gccggtcgcg gcggccggtg agcaccttgt gcgggtaggc
    13441 ctggtggccg acgtcccaga ggatcttgtc gcgcggcgaa tcgagcaggc tgtgcagggc
    13501 gaccgccagc tcgcacgtgc cgaggttggc gccgaagtgc ccgccgatct cgccgatcgt
    13561 gtcgatgatc agctggcgca cctcctgtgc cacctgcgcg agctgctcgt cgttcagccc
    13621 gtggaggtca ttcggtccgt cgatcttctc caggaggctc atgtttgtct cgtgaggatg
    13681 tagtcggtga tctgttcgag cgtctgcgtg cggccgccgg cgtcggcgag cgcggcacgc
    13741 gccttggcgt gcgactcccg cgctagctcc cttgccctgt cgagtccatg cacgctcacg
    13801 tatgtccgct ttccgtggcg ctgatcggag ccctgcggct tgcctaaggc cgcctcgtct
    13861 ccagtcacgt cgagtatgtc gtcgacgatc tggaacagca cgccaagctc cgctgcaaag
    13921 cgtcggtagg cgattgtcgc aggttccggc gcgcctgtaa gggcgagcac ggactgaacc
    13981 gacgcggcga tcagccggcc ggtcttgagc tcgtgcaggt gccggaggcc cgcctcgtcg
    14041 agatcgcccg gcgccgtgac gtccacgtac tggccgccaa ccatgcctga caccgctgcc
    14101 gcggacacca gctcgtgcac cgcggcgagc agattcgccg gctcgccctg ctggcgctcg
    14161 agcacgagct tgacggcctc ggcgaacagc ccgtcgccgg cgaggatcgc aacgtcctcg
    14221 ccgtagacca cgtggcaggt cggctgcccg cggcgaagct cgtcgtcgtc catggcgggg
    14281 aggtcgtcgt ggatcagcga gtaggtgtgg atgagctcga tggcggcggc gagcggcatt
    14341 acgtcgccac tcgggcgacc taccgcctcc gcagtggcga gcgccaaaac ggggcggatg
    14401 cgcttgccgc cggcgagcag cgagtagcgc atggcctcgc tcagcccggc ggtcgcgggc
    14461 tcgtcgccga agccgagctc cgagaggtag gcgtcgacct cggactgcag gtgggtcggg
    14521 tacccggccg cgggagccgc ggtgctcaca ggaggcgctc ctggcccggc acggactcgc
    14581 cgctcgcgac ggcgcgatcc cggcgctcca gctcgccggc gacctcgccg gcgaggtcgg
    14641 cgcagcgctc gacgagggcc gcggcctgct cgccctcgag ctcgcccgac ctcagttggg
    14701 aggccgcttg ctcgagctcc tggacgaggc gcgagaggct gtcttgtccg gcctccgcgg
    14761 tcactgactt gccaccgctt cccgctgcac tgagccaagt atgccgttga tgaaggctgg
    14821 agcctcggcc ccacacaatt ccttcgccgc ctcgaccgcc tcgtcgatcg cgacctcgtc
    14881 cggcacgtcg tcgcggtcga gtagttcgta gagcgccacg cgcagaatgt tccgctcgag
    14941 cggggcgatg cggtcaagcg tccagcccac cgagtggcgc tcgatcagct cgtcgaggcg
    15001 tggttgcacg gcggccgtgc actccacgag ctcgcgcgtg aacggcttgg cgttgcgatc
    15061 gacgatctcg tcgagcggcc ggccggtcac gtcgtgctga tagagcgcca cgacggctgc
    15121 cctgcgctgc tcggtgcgcc gcaagccgcc ctcctacgcg cgcgcgacgt actcgccgct
    15181 gcgggtgtcg acgcgcacgc ggtcgcccac gttcacgaag agcggcacgc gcacggccgc
    15241 gcccgtctcg agtgtggcgg gcttgtcgcc gccgccggag gccgtgtcgc cgcggaggcc
    15301 cggctccgtc tcggaaacgc tgagctcgac cgcgctcggc acctgcaggt ccgctgcgtt
    15361 gccgtcgatg tacaacacgt ccacttcctg gctcgggagg acccacttga gcgcgtcgcg
    15421 cacttgcccc tccgggacgg tgctctgctc gtaggtctcc atgtccatca ggtgggcgtc
    15481 ggtgccgtct gtgtaaagga actgcatctt gcggctctcg gttcgcacct ggcggaactt
    15541 ctcgccggcg cggaaggtct tgtcgatcac tgccccgtcg gccgttcgct tcagcttggt
    15601 tcggacgaac gcgccaccct taccgggctt gacgtgctgg aactcgacga tcttgaagac
    15661 cgtgccgtcc acatcgatgt ggctgccgtt cttgaactgg ttggtcgaaa tcacggggct
    15721 gaagggtaga cgccgtcagg acgctcgaga ttcgacccgg aaaacagggt gatcgggcgc
    15781 gatccgctgt aggtccgcgt cagacgagtc ggcgtccaca ccgccgaaga acgcgccgac
    15841 ttcccacttc catttcctca ggtacgcgcg cagaagctcg gttttctgct cgtctggtag
    15901 ctcggtagca gtaaaggact cactccgggg gccgactagc agtcttccct gaggagatgc
    15961 cctcaggttc ttcacccact gcgtgtgccc gcgcggggcg acgaggtagc gcccaccttc
    16021 gtaggtgagc agattcaccg gcgtgcgtcg cggctctccg gtcttgcggc ccttcacctc
    16081 gagtacgcgc gaaccagcca cgctgatgcc cacgcgcgtg agcgcggcga ctgtccggtt
    16141 gaacacgttc ttcgtgaacc agccggggcg ttgatagtgg tcgctcacca ccgcgccaag
    16201 gctagtggag caggcgccgc agatcggcgg cgatttctct cgcgtcgtac atgagctgct
    16261 gcgcggtgat gcggatcacc gtccagccgt tgaccgacag catccggtcg cgcgagcggt
    16321 ccttctcgaa cgcggcggtg cggtcgtggc tcatgcggtc gtcgagctcg acgatgaccc
    16381 gcttgtcgtg ccagacgcag tcggcctcga tccagcggtc ggggagctgg atcgggacgt
    16441 tcatctgggg ccgcggcagg ccgtgctcgt cgcagaaggc gatgaagagc tcctcgaggt
    16501 cgccccgtgt gatggtcgcg cccagttgcc ccgcttccag gattgctctt atcactgcgg
    16561 ttccccgtgc gcccgggtag cgctccaaga ggtcgggcaa tgagagcttg tcggtcaggc
    16621 gccgcacttc cgcctcgtca atggcgcgct ctacgtggcg tttgggcgct acggcggcaa
    16681 ggtcaaagat cgtgcgcggg atggtggtag ccgggatgcc gtcgtgggtc gtgacctcgt
    16741 cctcgggcag ttctcgccag tagaacttca gcggaccccg cgactgcttc tgccttggcg
    16801 ccgtgacctc gtgtgttgcg ggttgaaact tcagtaagca gtaaagagag gcagctgacc
    16861 gatggctcag gtgagcatca ggtcgccctg ccctcacagc cgccatccac tcgccttccc
    16921 gagtcagttt cgggtgcccg accgcgtaca cgccccggta cacgaggtgc agccgcccgc
    16981 tgtccacacg cttgcgaatg gcgccgcggc ccagcccaat ccctaaaagc tcatccctat
    17041 ccactacgcc gtgctgccgg gctgcgagct gcgctaataa aaggtctgcc gaacgacctt
    17101 taagttgagc accgtatagg tgctcaatat caatctcgtt cggcacgggc tcatcgtggc
    17161 ggcccggaac gcgcggttca gcgcgcgtgt gtgacgaatc agcgaccggg tgggcccggc
    17221 atcacgccga gctggcgcag catctccacg tcgtcgcgga tggcgaggtg ctcggtccat
    17281 ttgccctcgt cgccgctggt gaggatgtgc gcgtggcggg ccctgaagcc gttgttcgtg
    17341 ggcttcatgc cctcgaactc gcccgagtgc gtcccggtca tgtgacctac gcaggcgacc
    17401 ttgttgccgt cggcgatcac gtcctcgacc tcgaagcgca tgtcggggaa ggctgtccac
    17461 atccgctcga acgccattcg cgcgccctcc ggccccctcg gcgtgccctc aagagcattg
    17521 tgattcacga agtcatccga gacccagtcg ccgatcgagt cgatctcgtg tgcattgaag
    17581 acgtcctccc agaagcgaag cgcgcgttct cgaggctcct cggccacacc taccccgcct
    17641 ctttcaaccc gacgtcggcg gacccggctt caaggactcg cagcccgaac gcctcgaccg
    17701 gcccgagcgc acccgtcccc tcgagaccct ccgaggcagc gcgctgcgcg ccccaggcca
    17761 tgatgtcgcc ggtgaacgtg tagccgttcg gccccgagag ctcggcccgg gcgagcacgt
    17821 tgccgccgga gtcggccgcc tccgccggga accacgagcc cgtcttcgag cgagcctcgg
    17881 cgtcgggccc tccggtcgac cccttcacga agcgcgtcac gagcccgttg gcgagcgacc
    17941 gcatgccggg aatgtccccc gtcactgaca ccgcctggac agcgcgcgaa gccggcccga
    18001 accagccgag gtaggcatcc acctcgcgca aagatgggtg cacccgcggc aagccgaagt
    18061 gctcagaaga cccgatcgag atccccgtca tcgccctgcc gttgcggtcg aatgagcgca
    18121 cgcgcttcgc gggccgttcg gtcaccacgc gaccctcgcg gaacgcgaaa tgcggctcga
    18181 gcatcacgcc gcccatcgag gcgagcgtcc cgccgctcgc cgcgcccttg atcgacccgc
    18241 gcccggtgaa gaagtagccc acgctcacct tctccgcgtg ctcgccggct cgctcgagcg
    18301 ccagcgcgcc agcgaggttg cccggcacgt agtcgtagcc gaacgccgtg agcagtccgc
    18361 agcccgcctt gtccgctgca gccccgtact cctcgaacac cgcccggatg aacggcggct
    18421 cgccggtcga gtcgatgtag tgcgcgcccg cgtcgaccgc ggcctcgacc gccggccgcc
    18481 cccagcgcac gaacggcccg accgtgctca cgagcacgtc gccgcgctcc accagcgcgc
    18541 gcaccgactt cggatccgac acgtccgcca cctgcgtctc gagcccgccg cgctcggacg
    18601 ccagctgctc gagcttctcg cgcgagcgcg cggcgagcac cggacgcgcg ccgcgcttct
    18661 cgagtgcgtg cgccaccaag cgcccggtgt acccggtcgc gccgaacaga actatccggc
    18721 ctgccacgcg cgcagcctaa ccggaacgcg gttacggttg gcggatgacc gtgaccgagt
    18781 cgctccgaga aacgcgcgac cgcatcgtgc gagagcacat ggagtccgag aaccgtcacg
    18841 agttcgacgt gacgctcgcg acgttcgacc acccgcgcta cgagatcgtc ccgaccggcg
    18901 acgtctacga cgggccccag gaagtgtccg cctacttcac cgagagccgc accgcgttcc
    18961 cggaccagcg caacgagctg attgccctcc accacgccga tgacgcggtg atcgcggagt
    19021 tctggctgaa gggcacgcac cgcggtccgc tgcgcggtat cccgcctacc ggccgcgagt
    19081 tcagctgccg ctgcgtcgcc atattcgtgt tcgaggacga ccgcctggtg tgcgagcgcg
    19141 cctacttcga cgcggccacg atcctgaggc agctcacagg ctgacgcccg ggcgctcgac
    19201 gccctcgggc aagcggccct ccgacgccaa cttctccagg tgcgcggcga gcgtcgcctc
    19261 ggccgccggg cgaacgtgct ccatcaactg aggcggcacg tcgtcccacg cctgcgcgag
    19321 cagctcgtcg acgctgcgcg cgccgccgtc gagggcggcg atcagcttct cctcccgctc
    19381 catgcggtgc gcgatgtact cgtcgagctt cgccgccggg tcgcgcacca ccggcccatg
    19441 gcccgggcag agcagctcga gatcgagcga gcgcagcagc cgcagcgcgt acatatacgc
    19501 cgtcagccca ccgccctcgg ccgagatgaa cacgcttccc aacccaagca ccgcatcgcc
    19561 cgtgaagcag atcttgcggt agacgagcgc gacgtggtcc gcgctgtggc ctggcgtcgc
    19621 gatcgcgcgg aaggggccca cgtcgtcgcc gtccaccggc cacactacgg gcacgccccc
    19681 cgcgcgctcc gcgagctcag cggcgccgcc gtagtggtcg aggtggcgat gcgtgatcgc
    19741 gataccgtcg atccgaccac ccgccgcctc gagcagcgcc tcgacatgcg acgaatcgcg
    19801 cggccctgga tctaccaccc agtcgcgcac gatgtagctg ttcgtgccgc cgagagtgaa
    19861 ggccgacgcg ttcgacgctc tgacgcgcac gacgtcctcc gtgactctct ccacctccga
    19921 catctggcgc agatcttcgc agcccggata ggctccccgg cccacccgcg aagcaaggag
    19981 agcagatggc ccccgacgag ttgatgtgga agagcgcggc cgagcaggcc gagctggttc
    20041 gctcggggga agtgtcctcg cgcgagctcg tcaaggcgag cctcgaggcg atcgaccgcc
    20101 acaacgggga gctgaacgcc gtcgtgacac tcatgaccga ggaggcactc gagcaggccg
    20161 acgccgtgca gccaggggac gaccgcccgc tcgcgggggt gccaatgctg gtgaaggatc
    20221 tgatcgccct caccaagggc gtgcgcacga cgttcggcat gcgcgggatg ggcgagtggg
    20281 cgccgtcggt cgactcctcg accgtgcgcc ggctgcgcga cgcgggcgcg atcatcgtcg
    20341 gcaagacgaa cacgccggag atgggcatcc tgccggtcac cgagccgctc cgcttcggcc
    20401 cagcgcggaa tccctgggac acctcacgta cgcctggcgg ctcatccggt ggaagtggcg
    20461 cggcggtcgg ttcgggcatg gtcgcgatcg cgcacgggaa cgacggcggc ggctcgttgc
    20521 gcatccccgg gtcttgcaat gggctcgtgg ggctgaagcc gtcgcgcggg cgtatctcgt
    20581 gggcgccgga gttcgaggag ctggtcgcgg gcttcgcgat cgacggcgtg ctctcacgga
    20641 cggtgctcga caccgcaatc ggcctcgacg tgctctcggg ctacgagacg ggcgacccct
    20701 attgggcgcc cgacccgtcg gcgcccttcg tcgaggccgt cggccgcgag ccgggcaggc
    20761 tccgtgtgct gtggaccgcc cacgcgccga acggcgcgcc ggtcgacgac
//
LOCUS       DASVXS010000037         4771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_641949, whole genome shotgun sequence.
ACCESSION   DASVXS010000037 DASVXS010000000
VERSION     DASVXS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641949"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VF752_02075"
     CDS             <1..527
                     /locus_tag="VF752_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8854360.1"
                     /translation="QDREGVTPDLTILGKVIGGGLPAAAYAGRRDLMERVAPAGDVYQ
                     AGTLSGNPLAVAAGLATLNLLNADAYELLTQKTHVLAEGLERAAAEAGVPAAVVQAPG
                     LLTLFFREGAPRNLADVQECNFDAHAAFCRGMLERGVYLPPSQYEAWFPSLAHSDDQL
                     GQTVETAREVLAAL"
     gene            524..976
                     /locus_tag="VF752_02080"
     CDS             524..976
                     /locus_tag="VF752_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854361.1"
                     /translation="MSLLTEIVAAVDPTLAAYAVDEPGPGRFEDAPLERDRAFVLEVV
                     HEGYQMHYGEPRAFAGMDSDMRLLAGDALYALGLARLAESGDLEAVRELADLIALCAW
                     AHADKRPEVIDELWRASAEALSPKGGPGARAAVQERLVTGATGPAQSV"
     gene            1052..1726
                     /locus_tag="VF752_02085"
     CDS             1052..1726
                     /locus_tag="VF752_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX8854362.1"
                     /translation="MADTPQNPRKKKSPYTLDRGTPGAFEGETVTRRTLMSGGALAAG
                     GIATAAIALPALGFALGPVAEDTIQDNWRDVGPESDFNATTYVPKVITIVPEVGGEVG
                     KTTIYVRKAIAADKSPSDKGAPLPYVAISTRCMHLGCPVRYVQASQRFICPCHGGVYD
                     YEGKVVGGPPVRPLDRFYTKVENGRVLVGKRFSLDSHLRRYKPRDPSNHVDGLWKYLY
                     PSRPTT"
     gene            1723..2595
                     /locus_tag="VF752_02090"
     CDS             1723..2595
                     /locus_tag="VF752_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX8854363.1"
                     /translation="MTPPKPPLPNSLKRRPDRPGSQPLTGAPGGNGPNGRNGKAAVAL
                     RKQQQREKRKQQLLEIPISVVGWLDERSGVSPFLRAFMFRKTPRGTNWYYTLGSATLF
                     AFLSQAVTGVFLAMYYDPSPSGAYGSIRHIINEVWLGELVRGMHRWGATVMVVLIFVH
                     MGRTFVFGAYKYPRELNWVIGVVLLVLTLAMAFTGYLLPFDQRSFWATVVGVNINASG
                     PIVGPYLADFLRAGPEFTATTLSRFYAIHMLLIPVLIAALIGAHIYLVTKLGTTAPPW
                     LKAEKSRDLAKEEV"
     gene            2599..3309
                     /locus_tag="VF752_02095"
     CDS             2599..3309
                     /locus_tag="VF752_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8854364.1"
                     /translation="MDARQKEQYLREYSILKNQGKPFFPYAIMKDSLMACLVLAVIIL
                     LAILLGAELGPKADPTTTSYVPRPEWYFFFLFELLRIIKPPAIVFIATIGIPTICLVL
                     LILLPFIDRGPERNPLRRPIAITAAITVFAAMAYLTIEGAVAGAPGEITLKTAPQYEK
                     GKLVAAASGCQGCHKIGENGNAGPGPDLTKIGARLPRQAIARTLVNPTAPMPSYATLR
                     QKNPQQFNALVDFLASLR"
     gene            3317..4039
                     /gene="ubiE"
                     /locus_tag="VF752_02100"
     CDS             3317..4039
                     /gene="ubiE"
                     /locus_tag="VF752_02100"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX8854365.1"
                     /translation="MSARPSAEGGTGTLPEQQVRAMFDRIASVYDLMNSVMTVGMHHQ
                     WRERAADLARLGPGDRALDVATGTGDLAIALKRRVGPDGEVVGSDFSEKMLELARQKS
                     GDVRFEWANALELPYDDDSFDAATVGFGARNFADLAKGLVEMVRVVGPGGRVVVLELT
                     TPRRPPLSWFFTLWFDRAVPVLGRLAGDPDAYSYLPSSVKRFPGSHELAALMASLGLD
                     EVRYVLTAGGIVALHSGTVSQA"
     gene            4096..>4771
                     /locus_tag="VF752_02105"
     CDS             4096..>4771
                     /locus_tag="VF752_02105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8854366.1"
                     /translation="MLNAGGRRLIDLLVRTEGRLAELANGHGDGLARHAGETLTAGGK
                     RLRPITVFLCSGEADGEDLVRAATAVELVHMATLVHDDVLDRAMLRRGRATVFHAGGR
                     PAATATGDFLFSRAFEELVRTGSGDAVRTLSDASSALASGELMQRADAWSDDVSAERY
                     LERCELKTARLFWASCRLGALLEGNGNGSKVDALAAFGSRIGIAFQIFDDVLDVSGPV
                     ERTGKPR"
BASE COUNT          697 a         1700 c         1630 g          744 t
ORIGIN      
        1 cgcaggatcg cgagggcgtg acgcccgatc tcacgatcct cggcaaggtg atcggcggcg
       61 gcctgcccgc agcggcgtac gcggggcggc gcgacctgat ggagcgcgtg gcgcccgccg
      121 gcgacgtcta ccaggcgggc acgctctccg gcaatccgct cgccgtggcc gccgggctgg
      181 cgacgctcaa cctgctgaac gcggatgcct acgagctgct gacgcagaag acgcacgtgc
      241 tcgccgaagg tctcgagcgc gcggccgcgg aggccggtgt gcctgcggcg gtggtgcagg
      301 cgcccggact cctgacgctc ttcttccgcg agggcgcacc ccgcaacctg gcggacgtcc
      361 aggaatgcaa cttcgacgcc cacgccgcct tctgccgcgg gatgctcgag cggggcgtgt
      421 acctgccgcc gtcgcagtac gaggcctggt tcccctcgct cgcccacagc gacgaccagc
      481 tcggtcagac ggtcgagacc gcgcgggagg tgcttgccgc gctgtgagcc tgctcaccga
      541 gatcgtggcc gccgtcgacc cgacgctcgc cgcctacgcc gtggacgagc ccggcccggg
      601 acgcttcgag gacgctccac tcgagcgcga tcgggcgttc gtgctggagg tcgtccacga
      661 gggctaccag atgcactacg gcgagccgcg agccttcgcc gggatggact cggacatgcg
      721 cctgctcgcg ggcgacgcgc tctacgcgct cgggctcgcg cggctggccg agagcggcga
      781 cctcgaggcg gtccgcgagc tcgcggacct catcgccctg tgcgcgtggg cacatgccga
      841 caagcgcccc gaagtgatcg acgagctctg gcgcgccagc gccgaggcgc tgtcgccgaa
      901 gggaggaccc ggggcccgag cggccgtgca ggaacggctc gtgacaggtg ccacaggtcc
      961 cgcccagtcc gtgtgatcgc cggtgtcact ggggatgtaa tgtttccgcc cggcggcgcg
     1021 gatccgcgcg ccctggacac tatttctccc cttggcagac acaccgcaga accctcgtaa
     1081 gaagaagtct ccctacaccc tcgaccgtgg cacgccgggc gccttcgagg gtgagacggt
     1141 cactcgccga acgctgatgt cgggcggcgc gctcgccgcc ggcggcatcg ccactgccgc
     1201 catcgcgctg ccggcgctcg gctttgcgct cggcccggtc gccgaggaca ccatccagga
     1261 caactggcgc gatgtcggcc cggagtccga cttcaacgcg accacctacg tgccgaaggt
     1321 gatcacgatc gtgccggagg tgggcgggga ggtgggcaag accacgatct atgtgcgcaa
     1381 ggccatcgcc gcagacaaga gcccgagcga caagggcgcg cccctgccgt acgtggcgat
     1441 ctccacgcgc tgcatgcacc tcggctgtcc ggtgcgctac gtgcaggcgt cgcagcgctt
     1501 catctgcccg tgccacggcg gcgtctacga ctacgagggc aaggtcgtcg gcgggccgcc
     1561 cgtgcgcccg ctcgaccgct tctacaccaa ggtggagaat ggccgggtgc tcgtcggcaa
     1621 gcgtttctcg ctcgactctc acctgcgccg ctacaagccg cgcgacccgt cgaaccacgt
     1681 cgacggcctg tggaagtacc tctacccctc gaggccgacg acatgacccc tccaaagccg
     1741 cccctcccga actcgctgaa gcgccgtccc gaccgccccg gctcgcagcc gctcaccggc
     1801 gcgcccggcg gcaacgggcc gaacgggcgc aacggcaagg cggcggtcgc cctccgcaag
     1861 cagcagcaga gggagaagcg gaagcagcag ctgctcgaga tcccgatctc ggtcgtgggc
     1921 tggctcgacg agcgaagcgg tgtgagcccg ttcctgcggg cgttcatgtt ccgcaagacc
     1981 ccgcgcggca ccaactggta ctacacgctc ggctcggcga ccctgttcgc cttcctctcg
     2041 caggcggtca cgggcgtgtt cctcgcgatg tactacgacc cctcgccgtc gggcgcctac
     2101 gggtcgatcc gtcacatcat caacgaggtc tggctcggcg agctggtgcg cggcatgcac
     2161 cgctggggcg cgaccgtgat ggtggtgctc atcttcgttc acatgggccg cacgttcgtg
     2221 ttcggcgcgt acaagtaccc gcgcgagctg aactgggtca tcggcgtcgt gctgctcgtc
     2281 ctcacgttgg cgatggcctt caccggctac ctgctgccgt tcgaccagcg ctccttctgg
     2341 gccaccgtcg tgggcgtgaa catcaatgcc tccggtccga tcgtcggtcc ctacctggcc
     2401 gacttcctgc gtgcggggcc cgagttcacc gccaccacgc taagccgctt ctacgcgatc
     2461 cacatgctgc tgatcccggt cctcatcgcc gcgctgatcg gcgcgcacat ctacttggtc
     2521 acgaagctcg gcacgaccgc tccgccgtgg ctgaaggcgg agaagtcccg tgatctcgcg
     2581 aaggaagagg tctagcgggt ggacgcacgc cagaaggagc agtacctccg cgaatactcg
     2641 atcctgaaga accagggcaa gccgttcttc ccgtacgcga tcatgaagga cagcttgatg
     2701 gcgtgcctcg tgctcgccgt catcatcctc ctggcgatcc tgctcggcgc ggagctcggc
     2761 ccgaaggcgg atccaaccac cacgagctac gtgccgcgcc cggagtggta cttcttcttc
     2821 ctcttcgagc tgctgcgcat catcaagccg ccggcgatcg tcttcatcgc gacgatcggc
     2881 atcccgacga tctgcttggt gctgttgatc ctgctgccgt tcatcgaccg ggggcccgag
     2941 cgcaacccgc tgcgccggcc gatcgcgatc acggcggcga tcaccgtgtt cgccgcgatg
     3001 gcgtatctca cgatcgaggg cgcggtcgcg ggcgctccgg gcgagatcac gctgaagacg
     3061 gccccgcagt acgagaaggg caagcttgta gcagccgcct ccggctgcca gggctgtcac
     3121 aagatcggcg agaacggcaa cgccggacct ggtcctgacc tgaccaagat cggggcgcgc
     3181 ctgccacggc aggcgatagc gcggacgctc gtgaacccga cggcgccgat gccgtcgtac
     3241 gcgacgctgc gtcagaagaa cccccagcaa ttcaacgccc tcgtcgactt cctcgcctcg
     3301 ctccgctgac gctcctatga gcgcgcggcc cagcgcagag ggcggcaccg ggaccttgcc
     3361 cgagcagcag gtccgggcga tgttcgaccg catcgcgtcg gtctacgacc tgatgaactc
     3421 ggtcatgacg gtcggcatgc atcaccagtg gcgggagcgg gcggccgacc tcgcgcgcct
     3481 cgggccgggt gaccgcgcgc tcgacgtggc caccgggacg ggtgacctcg cgatcgccct
     3541 gaagcgccgg gtcggccccg acggcgaggt cgtcggctcc gacttctccg agaagatgct
     3601 cgagctggcg cgccagaagt cgggcgatgt gcgcttcgag tgggcgaacg cgctcgagct
     3661 cccgtacgac gacgacagct tcgacgccgc cacggtcggc ttcggcgcgc gcaacttcgc
     3721 cgacttggcg aaggggctcg tcgagatggt gcgcgtggtg ggccccggcg gacgcgtggt
     3781 ggtgctggag ctgaccacgc ctcggcggcc gccgctgtcg tggtttttca cgctctggtt
     3841 cgaccgcgcc gtgccggtcc tcggccggct ggccggcgac ccggacgcct acagctacct
     3901 gccgagctcg gtgaagcgct tcccagggtc gcacgagctc gccgcgctga tggcgtctct
     3961 gggcctcgac gaggtccgat acgtactcac cgcagggggc atcgttgccc tccactcggg
     4021 tacggtctct caagcgtgac ggctatcagc tctggggaca cgggctctgc gcctgccctc
     4081 gcgcagctag agcctgtgct gaacgcaggc ggacggcgcc tgatcgatct cctcgtgcgc
     4141 accgagggtc gtctcgccga gcttgcgaac ggtcatggcg acggcctcgc gcgacacgcc
     4201 ggcgagaccc tcacggctgg ggggaagcgg ctgcgcccga tcaccgtctt cctctgctct
     4261 ggcgaggcgg acggcgaaga cctcgtgcgg gcggctaccg ctgtcgagct cgtgcacatg
     4321 gccacgctcg tccacgacga cgtgctcgac cgcgcaatgt tgcgccgcgg tcgcgcgacg
     4381 gtattccacg ccggcgggcg ccccgccgcc acggctaccg gcgacttcct gttctcgcgg
     4441 gcgttcgagg agcttgtgcg caccggcagc ggcgacgcgg tgcgcacgct gtcggacgcg
     4501 tcgagcgcgc tggcgagcgg cgagctgatg cagcgcgccg acgcctggtc cgacgacgtg
     4561 agcgccgagc gctacctcga gcgctgcgag ctgaagacgg cccgcctgtt ctgggcgtcc
     4621 tgccggctgg gggctctgct cgagggcaac ggcaatggct cgaaggtcga cgcgctcgct
     4681 gccttcggct cgcgcatcgg gatcgcgttc cagatcttcg acgacgtgct cgacgtctcc
     4741 gggccggtcg agcgcacggg caagccacgc g
//
LOCUS       DASVXS010000038        10412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_712796, whole genome shotgun sequence.
ACCESSION   DASVXS010000038 DASVXS010000000
VERSION     DASVXS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712796"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VF752_02110"
     CDS             complement(<1..763)
                     /locus_tag="VF752_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8854367.1"
                     /translation="MPLQEVARRMKVLYERVAGIDVHKDMIKVAIRSPGDKQWTRKTE
                     ILEYRTFYGVLQQMARELRKRGVTHVVMEASGVYTEPVYYALCEQDFTEVAVINPAHA
                     KALKGHKTDARDCARLAELFECGLLRGSYIPAPELREVRDLTRYRMKTVQARTSEIQR
                     LAKALESAGIKLGSVASSITGASATAMIEALIDGERRGGVLADLAKGRMRAAGKLADL
                     SMALAGRFTGHHALLCRLHLDRIAVFDDAVDGLDKR"
     gene            complement(1152..1466)
                     /locus_tag="VF752_02115"
     CDS             complement(1152..1466)
                     /locus_tag="VF752_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8854368.1"
                     /translation="MLAKIAEMQEQDRAMAERLHTIVKSAAPDLSPRTWYGMPAYAKD
                     GKVVCFFQSAQKFNTRYATLGFSDEANLDEGSMWPTSFALKELGATEEARISALVKKA
                     VS"
     gene            complement(1865..3988)
                     /locus_tag="VF752_02120"
     CDS             complement(1865..3988)
                     /locus_tag="VF752_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8854369.1"
                     /translation="MARIAAFVNRRAKRILVVAGVFFVVAGVVGGPVAGQLSAEDEDF
                     QDPSAENIVANERLVAAGGGSEDAGLVALVSPGGDVRRDPDARVRVRAIQRTIEADPG
                     VVAARSYIDTRNPALVSRDGRQTLVLAAFTDDELEAADRLRSKLAGQGVKIGGPDVVQ
                     PEIGDRVSSDLARAELIAFPLLFLLSLWVFRSLVAALLPPLVGALSIVTTFLLMRFVD
                     SSVTSLSIYALNLVTGIGLGLAIDYSLFMVSRYREELAQGNAGVAAITRTLQTAGRTV
                     LFSSLTVAAALAALLVFPLRFLYSMGIGGVIVALVAMLVSLTVLPAVLAALGPRVNAG
                     APRFLRRAADSAARPATSGGWYRLAMWVMRRPAAVAIVTAGVLLVAGLPFLRVAFVPA
                     DYKMLPQGSESRQVAETIDRNFGTFAADPIQVVARATPTEDDQVARYASALRDEPGAG
                     AVSEPRRLSSNTVLIDVLPPGDALSKRNRELVRTVRAVDAPVPVAVGGAAARFVDQQD
                     TLGDYLPFALALLALTTFVLLFLLTGSVILPLKALLMNVLTLSATFGILVLVFQDGRF
                     EGVLDFVSAGGVEATQPVLLFAIAFGLATDYGVFLLSRIKEAHDSGLENRAAVAFGVE
                     RTGRIVTAAALLFCVAIGAFATSGILFIKLLGLGTALAVAIDATVIRSLLVPALMALL
                     GKWNWWAPAPLRRLHARIGLSEAPT"
     gene            complement(4082..4759)
                     /locus_tag="VF752_02125"
     CDS             complement(4082..4759)
                     /locus_tag="VF752_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854370.1"
                     /translation="MIRVALVDDQAMVRTGLARILSPADGFDVVAECADGQQAVEELP
                     HVHPDVVLMDIRMPRLDGIAATAELRRAANAAPVLVLTTFGEDEVLWGAIEAGAAGFV
                     LKDSSAEDLIAAVRAVAGGAAWFDPGVTPRVLERYRQTVAPAIRDAARLDELTDREHE
                     VLRLMARGATNAEIAATLFVAEATVKSHVGSIFMKLGVRDRAAAIVFAYDHGEVRPGP
                     PRPPAAA"
     gene            complement(4756..5919)
                     /locus_tag="VF752_02130"
     CDS             complement(4756..5919)
                     /locus_tag="VF752_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8854371.1"
                     /translation="MSGGLRPEADQTDQLRALIPVCALGVALAAAVGDPSSATDLLLV
                     VVAVGAFAVWAHVRNVPLWALSLGVLVPVVVTQRSGALEPLFFEASILAFVVGGWARS
                     LVEAVALGLLTVASPVVVSLIQDPSEIAVGIWVLGIAFPWVIGLAMARQRHLTAELDA
                     ARRELAEQALLDERRRIARDVHDFVGHGLAAVMLQVTSARHVLRRDSDAAEDALRSAE
                     EVGRRSMQELRRTLTLLRSDDEPEVAPPLPSSADIPALVDHARAGGLAVEFRTRGDGV
                     RIGPTVGLALYRIAQEALANAARHAPRAQTVLVLDVADAQASLVAETIGPMLPGAASD
                     PQRPRYGLIGMQERVAAFGGEFAAGPTSDGWRVSCRIPLEATDAASAGDSGAR"
     gene            complement(6010..7095)
                     /locus_tag="VF752_02135"
     CDS             complement(6010..7095)
                     /locus_tag="VF752_02135"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX8854372.1"
                     /translation="MAAASVLKRRGHEVVVLVSGETRDAANQLGFEVTSYRRSPDPDV
                     SVAFEDQADLLMATMAGAEIALDARAVLNELRPDLAVVDCMLPAAIAAARSTGTPTVS
                     LVHFLYGLARTQMLQAGGGWTTDLHSLAETHRTLGLAPALDGLTAWEAPELVLVTAPR
                     WLDIDCDAPPNVVHAGPLGVAVASQRQGRADSSRKRVLLTFSSTVMHGQAALIERVSE
                     AVADLELDAVLTLGSVVDRDAVRVPEGVEVIAFGDHDRLMPGMAAVIGHGGLGTVLRA
                     LAHGVPHLVLPLGRDQAFNAARVAQIDGGIQLATDAPAGRIRTALSALLSDSRFAAGA
                     TRAARRIAADEADRTAAEAVERTARQE"
     gene            7283..7933
                     /locus_tag="VF752_02140"
     CDS             7283..7933
                     /locus_tag="VF752_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8854373.1"
                     /translation="MGKIVISENVSLDGVIEDPTGAEGLRVGGWFFETDRGEDGERFT
                     LDKTLSTEALLLGRRSYEFFAATWPSRGGELADKLNSLPKYVVSSTIEDPDWNNSTVL
                     TGDVVEEVSKLKRELSGEIVVLGSPQLARTLIEHDLVDELRLMIYPVQLGAGARLFGE
                     TSDKKPIRLVDTQTVGDGIAILTYERVRDAERAPDGANLQDKRAAALRQAGIRESA"
     gene            complement(8000..8887)
                     /locus_tag="VF752_02145"
     CDS             complement(8000..8887)
                     /locus_tag="VF752_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8854374.1"
                     /translation="MDLRQLSYFVAVAEEGQFTRAANRVSVAQPAVSAQIRRLERELG
                     EALFHRDQRAVTLTGAGEALLPHARAALAAAERGRDTIASLRDMLHGRLSVGVAGPVD
                     DRFAEALGAFHRAHPAVEIALTQQHNEPLLAAVADGAIDAAIVGAGVQPLPPRVRTRV
                     VATEPLVLAVRRGDPLSSRRTVTLAQLRDQPMITLVRGSGLRTVLESACRDAGFIPRI
                     TAEAGELTSLSELAAEGLGVAILPRSATDGADLAVLKITRPRLRRRTALAWNEATTSP
                     AARAFLALANKRFDSSPSR"
     gene            8959..9267
                     /locus_tag="VF752_02150"
     CDS             8959..9267
                     /locus_tag="VF752_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854375.1"
                     /translation="MREYIDNRPGARRWLAPVATTVGAWLVAFLVVTSPLSVFGDELG
                     SLSLMPRAAVISGVLVAFMVNVVMPVLSAAVAHWMGRPPQTRPDAGRPRTTSGEEGRA
                     "
     gene            9248..9727
                     /locus_tag="VF752_02155"
     CDS             9248..9727
                     /locus_tag="VF752_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854376.1"
                     /translation="MRRAVPDRNTPTAREHPQLEVLPDWPTRTIAVLATVDLGPHAIP
                     ISAPVRAGDRRILLSLRHGRGSLARLRSQSSVALAILTERNIAFTAHGHARIVRDQLA
                     GASDYAAVAIDVEHIDDHRQADFRIESGIDRSWVDGNEQSALARRVESLRELATTDR"
     gene            complement(9758..>10412)
                     /locus_tag="VF752_02160"
     CDS             complement(9758..>10412)
                     /locus_tag="VF752_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854377.1"
                     /translation="VLGTKLAVRSGTKLVVAGGLVALAAGLAWTSSASTGTSYLTIIG
                     QMVLIGAGIGLTSAPATESIMGAVPAAKAGVGSAINDATRILGATLGVAVIGSVYASI
                     YGSRLGSALSAQLPPAAEDVAHKSVGGAFQAAAQLDSGHHAALARSLHDAASKAFFDG
                     FSVACLVATGVALLGAVMAAALIPPQPPQARTEAGETLSGRRGSSVPDTPTRACCSC"
BASE COUNT         1723 a         3619 c         3537 g         1533 t
ORIGIN      
        1 tccgcttgtc caggccgtcg accgcgtcat cgaagacggc gatccggtcc aggtgcaggc
       61 ggcacagcag cgcgtgatgc ccggtgaacc ggccggccag cgccatcgac aggtcggcca
      121 gcttccccgc cgcccgcatc cggcctttgg ccaggtctgc cagcacgccg ccgcggcgct
      181 ccccgtcgat cagcgcctcg atcatcgcgg tggccgacgc gccggttatg ctgctggcca
      241 ccgagccgag cttgatgccg gccgattcca gcgctttagc tagccgctgg atctccgagg
      301 tgcgggcctg gaccgtcttc atcctgtacc gggtcaggtc ccgcacctcc ctcagctccg
      361 gcgccgggat atacgacccg cgcagcagcc cgcactcgaa caactccgcc agccgggcgc
      421 agtccctggc gtcggtcttg tggcccttca gcgccttggc gtgcgccggg ttgatcaccg
      481 cgacctcggt gaagtcctgc tcgcacagcg cgtaatacac cggctcggtg tataccccgc
      541 tggcctccat caccacgtgg gtcacgcccc gcttacgcag ctcgcgggcc atttgctgga
      601 gcaccccgta aaaagtccgg tactccagga tctcggtctt gcgggtccac tgcttgtctc
      661 ccggggaccg gatcgcgacc ttgatcatgt ccttgtgcac gtcgatcccg gcgacgcgct
      721 cgtatagcac cttcatgcgc ctggccacct cctgcagcgg caccccccac ggataggaca
      781 aggcgcgccg ccccgccaga gccccggatc agtacaggag actgacacac gcgcacgcag
      841 cagcaacggg ggttacccgg catggggctc cggcagcaat ctaatcaacg gcctcgaacg
      901 gtccataggc agtcggtgac ggcaggcggc gcgctagcac aacagtgcac cttccattcg
      961 gcccgccgcc agccgtcacc cccccgaaag accccggcca gccacgatcg gcactcaccg
     1021 acggcgcagg acccagtttc cggatcccac ggcagcgcca cgccagcggc gcatgatttc
     1081 taatcaccca atggctgccc aggcgcctat ggaacacggc ctagggcggg gtccagtggc
     1141 ttgctcagtc ctcagctcac cgctttcttc acgagcgcgc tgatccttgc ctcttcggtg
     1201 gcgcccaact ccttcagcgc gaaggaggtc ggccacatgg agccttcgtc gaggttcgcc
     1261 tcgtcgctga agccgagcgt cgcgtacctc gtgttgaact tctgcgcgct ttggaagaag
     1321 cagacgacct tgccgtcctt ggcatacgcg ggcatcccgt accaggttct cggcgagagg
     1381 tcaggcgcgg cggatttgac gatcgtatgg agccgctcgg ccatggcgcg atcctgttcc
     1441 tgcatctcgg cgatcttcgc gagcacgtcg ctttccccgt ccgccttgct cgcgcgcccg
     1501 ccgcggcgcg cggccgcctt ctgctcttga acgcgctccc ccatcgcggc gcgttcctcg
     1561 tcagtgaatc ccttggacgc cttgccggtg gttctcccgg cggatcgctg cgtggccttc
     1621 tttgcggatt tcctctcagc cattgcgtag caccttcgtg gtcgagtaaa tcgtgatgtc
     1681 gtggcggtca ggcaagttgc ggcccggcga cctgcgcttc ccgcttcgta atcggtcgcc
     1741 gctcacgcgc cgaccaagcc cactccgagg gtggccgcgc cgatgctgag cgacaggccg
     1801 ccaagtggga cgaaatagat gggcgaaatg aactgagtca gtggacatcg tgatgcctcc
     1861 ctcatcaggt tggcgcctca ctgagcccga tccgggcgtg gaggcgacgc agcggcgcgg
     1921 gcgcccacca gttccacttg ccgagcagcg ccatcagagc agggacgagc agagagcgga
     1981 tgactgtcgc gtcgattgcc accgcgagcg cggttccaag gccgagcaac ttgatgaaca
     2041 ggatgcccga cgtggcgaag gcgcctatgg cgacgcagaa caacagcgct gcggccgtca
     2101 cgatccgtcc cgtccgctca accccgaacg ccaccgccgc acggttctcg agaccgctgt
     2161 cgtgcgcttc cttgattcgc gacagcagga atacgccgta gtcggtcgcc aggccgaagg
     2221 cgatcgcgaa cagcagcacg ggttgcgtcg cctccacgcc gcccgcgctc acgaagtcca
     2281 gaacgccctc gaaccggccg tcctggaata cgagcacgag gatcccgaac gtcgcgctga
     2341 gcgtgaggac gttcatgagc agcgccttga gcggcagaat gaccgagccg gtcagcagga
     2401 acagcagcac gaacgtcgtg agcgccagca gcgcgagcgc gaacggcagg tagtcgccaa
     2461 gcgtgtcctg ctgatcgacg aacctcgcgg ccgcgccacc gaccgccacc ggcaccggag
     2521 cgtcgacggc gcgtacggtc cggaccagct cgcggttgcg cttcgagagc gcgtcgcccg
     2581 gcggtagaac gtcgatcagc accgtattgc tgctcaagcg ccgcggctcg gagaccgcgc
     2641 cggcgcctgg ctcatcgcgg agagcgctgg cgtagcgtgc cacctggtcg tcctcggtcg
     2701 gcgtggcccg cgcgacgact tggatcggat cggcagcgaa cgtgccgaag ttgcggtcga
     2761 tcgtttccgc gacctggcgt gactccgagc cctgcgggag cattttgtag tcggcgggaa
     2821 cgaaggccac gcgcaggaac ggcaagcccg cgacgagcag cacgccggcc gtgacgatgg
     2881 ccacggcggc gggccgccgc atgacccaca tcgcgaggcg ataccagcca cccgatgtcg
     2941 ccggccgtgc cgccgagtcc gccgcgcggc gcaagaaccg gggtgcgccc gcgttgacgc
     3001 gcgggccgag ggcggcaagc accgccggga gaacggtgag cgacacgagc atcgccacga
     3061 gcgccacgat gacgcccccg atgcccatcg agtacaagaa gcggagcggg aagacgagca
     3121 gtgccgcaag cgctgcagcg acggtcagag agctgaacag cacggtccgg cccgccgtct
     3181 gcagcgtccg agtgatggcc gccacgcccg cgttgccctg ggcgagctcc tcgcgatagc
     3241 gcgagaccat gaacaggctg tagtcgatcg ccaggccgag gccgatgccg gtcacgaggt
     3301 tcagcgcgta gatcgacagg ctagtgacgc tcgagtcgac gaagcgcatc agcagaaacg
     3361 tcgtgacgat cgacagtgct ccgacgagcg gaggcaggag cgccgccacg aggcttcgga
     3421 acacccacag cgaaaggaga aacagcagtg gaaaggcaat gagctccgcg cgagcgaggt
     3481 cggacgaaac ccggtcaccg atctccggct ggaccacatc ggggccgccg atcttcacgc
     3541 cctggcccgc gagcttcgat cgcaaccggt ccgcggcctc gagctcgtca tcggtaaacg
     3601 ccgcaagcac gagcgtttgt cgcccgtcgc gcgagacgag cgccgggttg cgggtgtcga
     3661 tgtaactgcg cgctgcgacg acgccggggt cggcctcgat cgtacgctgg atcgcccgga
     3721 ccctcactcg agcgtcggga tccctcctta cgtcgccacc gggcgacacc agcgcgacga
     3781 gcccggcatc ctcgctgcct ccgcccgccg cgacgagtcg ttcgttcgcg acaatattct
     3841 ccgcgctcgg gtcctgaaag tcctcgtcct cggcgctcaa ttggccggcc accgggccgc
     3901 cgaccacgcc tgcgactacg aagaagacgc cggcgacgac caggatccgc ttcgcgcgac
     3961 ggttgacgaa tgcggcgatc cgagccatgg ccgcgacgtt aggtcgcggc gtggcgcttg
     4021 gcatcgaccc atgggatcta cctgcgtccg accttcgtcg gaggggcgcc gaccgctggg
     4081 actaggcggc ggcgggcgga cgcggcgggc cgggacgaac ctcgccgtgg tcgtacgcga
     4141 agacgattgc cgcggctcgg tcgcggacac cgagcttcat gaagatgctg ccgacgtgac
     4201 tcttcaccgt cgcctccgcc acaaagagcg tcgcggcgat ctcagcgttc gtcgcaccgc
     4261 gtgccatcag ccggagcacc tcgtgctcgc gatcggtgag ctcgtccagg cgggccgcgt
     4321 cgcggatcgc gggcgccacg gtctggcggt accgctcgag cactcgcggg gtgaccccgg
     4381 ggtcgaacca ggcagcgccg ccggcgacgg cgcgcacagc cgcgatgaga tcctccgccg
     4441 agctgtcctt gagcacgaac ccggcggcgc cggcttcgat ggctccccac agcacctcgt
     4501 cctcaccgaa cgtcgtgagg acgaggaccg gggccgcgtt cgctgcccgg cgcaactctg
     4561 cggtcgccgc gataccgtcg agcctcggca tgcgtatgtc catgaggacg acgtctggat
     4621 gcacgtgcgg gagttcttcg acggcctgct ggccatcggc acactccgcg acgacgtcga
     4681 acccgtcggc cggggaaagg atccgagcca agcccgtccg gaccatggcc tgatcgtcga
     4741 cgagcgcgac ccggatcatc gggcccccga gtccccggcg gacgccgcgt ccgtcgcctc
     4801 caacggaatc cggcagctca ctcgccagcc gtcggatgtc ggccccgcag cgaactcgcc
     4861 gccgaacgcg gccacccgct cctgcatccc gatcaggccg taccggggtc gctgcggatc
     4921 ggaagccgcc ccggggagca tcggtccgat cgtttcagca acaagagacg cctgcgcgtc
     4981 cgccacgtcg agcacgagaa cggtctgagc gcgcggcgcg tgccgcgccg cgtttgcgag
     5041 cgcctcttgc gcgattcggt agagcgcgag ccctacggtc ggcccgatgc gtacgccatc
     5101 ccctcgcgtc cgaaactcga cggcgagccc gcccgcccgc gcgtggtcga ccagtgcggg
     5161 gatatcggcc gacgacggga gtggcggcgc cacctccggc tcgtcgtcgc tgcgtagcaa
     5221 ggtgagcgtg cggcgcagct cttgcatgct ccggcggccg acctcctcgg cagatcgcag
     5281 tgcgtcctcg gcggcgtcgc tgtcgcggcg cagcacgtgc cgggcgctcg taacttggag
     5341 catgactgca gcaagcccgt gtcccacgaa gtcgtgaaca tcgcgagcga tccgacgtcg
     5401 ctcgtcgagc agtgcctgct cggcgagctc gcgccgcgcg gcatcgagct cggctgtcag
     5461 gtgcctttgg cgagccatcg caaggccaat gacccacgga aacgcgatac ccaggaccca
     5521 gatgccgaca gcaatctccg acgggtcctg gatcaggctc acgaccactg gcgaggcgac
     5581 ggtcagcagc ccgagcgcta ccgcttcgac gagcgaccgc gcccagcctc caacgacgaa
     5641 cgcgagaatg gaagcctcga agaagagtgg ctcgagcgct ccagatcgct gcgtcacaac
     5701 cacggggacg agcacgccaa gcgacaacgc ccagagcggc acgttgcgca catgcgccca
     5761 cacggcgaaa gcgccgaccg ctactacgac aaggaggagg tccgtcgccg acgacgggtc
     5821 gccgaccgcg gcagcgagcg cgactcctag cgcgcagacc gggatcagcg cgcgcagctg
     5881 gtcggtttga tctgcctccg gacgtaatcc tccggacacg gctcgaccat actgcggcac
     5941 gttgctccag acatcccccg cgagtctgag gcggatctcc gacgacgggc ggagctcacg
     6001 ccggtgcggt cactcttgcc gggctgtgcg ctcgaccgcc tcagcggcgg tccggtcggc
     6061 ttcgtcggcg gcgatgcggc gggcggcgcg ggtggccccc gccgcgaatc gcgagtcact
     6121 caagagtgcg ctcagggcgg tccggatccg cccggctggc gcgtccgtgg cgagttggat
     6181 cccgccgtcg atctgcgcga cgcgggccgc attgaaggcc tggtcgcgcc ccagcggcag
     6241 caccaggtgc ggcacgccgt gtgcgagtgc gcgcagcacc gttcccaatc cgccatgtcc
     6301 gatcaccgcc gccatgccgg gcatgagacg atcgtggtcg ccgaaggcga tgacctcgac
     6361 accctcgggg acccggacgg catcccggtc gaccaccgag ccgagcgtga gcacagcatc
     6421 cagctcgaga tcggcgactg cctcgctgac gcgttcgatc agtgccgcct gaccgtgcat
     6481 cacggtgctg ctgaacgtaa gcagcacccg cttgcgcgag ctgtcggccc ggccttgccg
     6541 ctgcgaagcg acagcgaccc ccagcggccc agcgtggact acgttcggcg gtgcgtcgca
     6601 atcgatgtcc agccagcgag gcgcggtgac gagcaccagc tcgggggctt cccaagccgt
     6661 gaggccgtca agggcgggtg cgagtcccag cgtccgatgc gtctccgcga ggctatgaag
     6721 atcggtcgtc cagccgcctc cggcttgcag catctgggtt ctcgcgaggc cgtagaggaa
     6781 gtgcacgagc gagacggtcg gcgtacccgt ggaccgagca gccgcgattg cggccgggag
     6841 catgcagtcg acgaccgcga ggtcggggcg cagctcgttg aggacggcgc gggcgtcgag
     6901 ggcgatctcc gcgccggcca tggtcgccat caggagatcc gcctggtcct cgaatgcgac
     6961 actgacgtcc ggatcggggc tccgccggta ggaggtcacc tcgaacccga gctggttcgc
     7021 ggcgtcgcgc gtctcgcccg agacgagaac cacgacctcg tggccgcgcc gcttgagcac
     7081 agaagccgcg gccatcatcg gaggcaggtt cccgccaggt gcccaagtaa ccacgagcgc
     7141 gcgcacaatc gcagcgaatc acggggacgc gatggggaga ccgtaccgat cgaaggcgcg
     7201 gaggttgccc cgtacgagcg gccgttccgt tatcgccgcc gtcggtgtct gaactgataa
     7261 cgccgacgac aggaggatcg aaatgggaaa gatcgtcata agcgagaacg tctcgctcga
     7321 cggcgtcatc gaggacccga cgggcgccga gggcttaagg gtcggcggct ggttcttcga
     7381 gaccgatcgt ggcgaggacg gcgaacggtt cacgctcgac aagacgctga gcaccgaggc
     7441 cctgcttctg ggtcgtcgga gctacgagtt cttcgccgcg acctggccat cccggggtgg
     7501 cgagttggca gacaagttga acagcctgcc caagtacgtg gtgtcctcga ctatcgagga
     7561 tcccgactgg aacaactcga cggtactgac gggagacgtg gtcgaagaag tctcgaaact
     7621 gaagcgagag ctgagcgggg aaatcgtcgt cctcggcagc ccccagctcg cgcgtacgct
     7681 gatcgagcac gacctcgtcg acgagctgcg gctgatgatc tacccggtcc agctcggggc
     7741 cggcgcgcgc ctctttgggg agacgagcga caagaaaccc atacgcctcg tcgacaccca
     7801 gacggtcggt gacggcatcg ccatcctcac ctacgagcgc gtccgagacg ccgagcgggc
     7861 gccggacggc gccaaccttc aggacaagcg cgcggccgct ctccgccagg ccggcattcg
     7921 ggaaagcgcc tagagagcag cgagcgacaa ggagggccgc ggcgctggcg attcaccgca
     7981 acggacagcg ggcgtccgct caccggctag gtgaggagtc gaagcgtttg ttggcgagcg
     8041 ccaggaacgc ccgcgctgcg ggcgacgtgg tggcctcgtt ccaggcgagg gccgtgcgtc
     8101 ggcgaaggcg cgggcgggtg atcttcagaa ccgcgaggtc tgctccgtcc gtggcggagc
     8161 gcggaaggat cgccacgccg agcccttcgg cggcgagctc gcttagggag gtgagctccc
     8221 ccgcctcggc agtgatcctg gggatgaagc cggcatcccg gcaggcgctc tcgagcaccg
     8281 tgcgcaggcc gctgccgcgc accagcgtga tcatcggttg gtcacgtagc tgagcgaggg
     8341 tgacggtcct gcggctgctg agcggatcgc cgcggcggac cgcgagcacg agcggttcgg
     8401 tggcgaccac gcgcgtgcgg actcgtggcg gcagaggttg aacgcctgcg ccgacgatcg
     8461 cggcatctat ggcgccgtcg gctacggctg cgagcagcgg ctcgttgtgc tgctgggtga
     8521 gcgctatctc gaccgcggga tgggcgcgat ggaaggcgcc gagcgcctcg gcgaagcgat
     8581 cgtcgacggg ccccgcgaca ccgacactca gtcgcccgtg gagcatgtcg cggagggaag
     8641 cgatcgtgtc gcggcctcgc tcggcggcgg cgagagcggc gcgcgcgtgt ggcagaagcg
     8701 cttcgcctgc tccggtaaga gtgactgcgc gctgatcgcg gtgaaagagc gcctccccga
     8761 gctcgcgctc caggcgacgg atctgtgcgc tgacggccgg ctgggccacc gacacccggt
     8821 tagccgcgcg cgtgaattgc ccctcctcgg ccacggcgac gaagtacgag agctgcctaa
     8881 ggtccataac gatacgttct gccgcatagt ttaaacctgt ctttgactta tgagcggatc
     8941 gtcgcctacc gttcgcctat gcgcgagtac atcgacaatc gccccggcgc tcgccggtgg
     9001 ctggcgccgg tagcgacgac ggttggagcg tggctcgtgg cgttcctcgt cgtgacctcg
     9061 cccctctccg tgttcggcga cgagctgggg tcgctgtcgc tcatgccgcg ggcggcggtg
     9121 atcagcggcg tgctggtcgc ctttatggtc aacgtggtga tgccggtcct gagcgcggcc
     9181 gtcgcccact ggatgggccg tccgccgcag acgcgcccgg acgcggggcg cccgaggact
     9241 acttctggtg aggagggccg tgcctgaccg caacaccccc actgcgcgcg agcacccgca
     9301 gctcgaggtc ctcccggatt ggccaacgag aacgatcgcc gtactcgcca cggtggatct
     9361 ggggccccac gcgatcccga tctccgcccc ggtgcgcgcc ggggatcgcc gcatcctgct
     9421 gagcctgcgt cacggccgcg gatcgcttgc gcgcctgcgt agtcagtcca gcgtggcgct
     9481 cgccatcctc accgagcgaa atattgcctt taccgcacac ggccacgcgc gaatcgtccg
     9541 agatcagctg gcaggggcat ccgactacgc ggcggtcgcg atcgacgtcg agcacatcga
     9601 tgaccatcgc caggcggact tccgcatcga gtccggcatc gaccggagct gggtcgacgg
     9661 aaacgagcaa agtgccctcg ctcggcgggt cgagtcgctc agagaactcg cgaccaccga
     9721 caggtagatg gccccgggac cgtcgggcag acgagcgtca gcacgagcag cacgcccgcg
     9781 tcggcgtgtc cggcacggaa gaacccctcc tgccggaaag ggtttcaccc gcctcggtgc
     9841 gtgcctgcgg cggttgcggc gggatgagcg cggcggccat tacggcccca agtagggcga
     9901 ctccggtcgc aaccaagcaa gcgactgaga acccgtcgaa gaacgctttc gaggcggcgt
     9961 cgtggagcga ccgggccagc gccgcgtggt gacccgaatc gagttgagcg gcagcctgga
    10021 aggcgccgcc caccgacttg tgtgcaacat cctcggcagc cggcggcagc tgcgcggaca
    10081 gcgcggagcc aagccggctg ccgtatatcg aggcgtagac gctgccgatc acggcgacgc
    10141 cgagggtcgc tccgagtatc cgggtggcgt cgttgatggc cgaccccacg cccgccttcg
    10201 ctgcgggcac cgcgcccata atcgattcgg tcgccggggc actcgtcagg ccgatccccg
    10261 cgccgatgag caccatctgg ccgatgatcg tcaggtagct ggtgcccgtg gacgccgagg
    10321 atgtccacgc cagtccggcg gcaagggcca cgagcccgcc cgctacgacc agcttggtgc
    10381 cgctgcgcac cgccaacttc gtgccgagga cc
//
LOCUS       DASVXS010000039         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1142735, whole genome shotgun sequence.
ACCESSION   DASVXS010000039 DASVXS010000000
VERSION     DASVXS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142735"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF752_02165"
     CDS             complement(<1..345)
                     /locus_tag="VF752_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854378.1"
                     /translation="MAAPGRTAAELSEQLLAGDKRALARAISLVENDDPEGWALVREV
                     YPRTGNAAMVGFTGPPGVGKSTLIGALVKEARSKDREVAVLSIDPSSPFTQGALLGDR
                     IRLTEHFLDPGVF"
     gene            464..1015
                     /locus_tag="VF752_02170"
     CDS             464..1015
                     /locus_tag="VF752_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8854379.1"
                     /translation="MLDAALDAFDEHGFSGASIEEVRHRSGASIGSIYHHFGGKEGLA
                     AELYVGGLRSYQEGLLEALEQSASAQAGVEAAVRHHLRWIGQNPKLARFMFENSIATP
                     ELRAQNRRFFAAVHEWLERQAERGEIRRLPGDLYYALLIGPSQELARHSLAGRIEIPL
                     GDAADELAHAAWMSLRNDQGGPS"
     gene            1012..1842
                     /locus_tag="VF752_02175"
     CDS             1012..1842
                     /locus_tag="VF752_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8854380.1"
                     /translation="MTVTVYEDIVDAARADTRELVRVERDGDHAIVRMDDPESLNALS
                     APLTLQLHDALAALAADRAIRSIVLTGSDPAFSAGGDLRLMQDAHEGIDEGDQGATLA
                     WRWIRYQFGGIARLITRTDKAFIAAVNGAAAGVGLAFALACDIVIASERARIVPAFMK
                     IGLLPEVGTSWHLTRRIGYQRTFELFAGGRILSAQEADDLGMVNAVVPHDALMDAALE
                     WCKRISQLPPHALAMTKPLLRNAADMPWEGAITMEELAEPNCFTTTAHRGAVQALLAH
                     "
     gene            complement(2050..2178)
                     /locus_tag="VF752_02180"
     CDS             complement(2050..2178)
                     /locus_tag="VF752_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854381.1"
                     /translation="MLAHLSSVIGVILGLLLGLHGALLHLPGSLRGIVRALHPDLD"
     gene            2191..2763
                     /locus_tag="VF752_02185"
     CDS             2191..2763
                     /locus_tag="VF752_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX8854382.1"
                     /translation="MTEVNRETERQVKQRVAEAEKAAAEAVKGERRAAEADAEEERQE
                     VRGEVDEEIEAAERDAEAARQRAQELVADATEQLADARRLAEEAAEAARAAAEEAHRQ
                     AQQLADEAQQQASDAEARVKAAEQVREQSAGAARHTARELERNPTNGGLQSYNKPELI
                     ELAASIGIEGRRSMSKGELVDAIAKASRRR"
     gene            2781..3005
                     /locus_tag="VF752_02190"
     CDS             2781..3005
                     /locus_tag="VF752_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854383.1"
                     /translation="MQLFNRKSELERMLEKLTDPLEATSKLTFDMPSGGAGKALKVGL
                     ISAGGLAGLTAASAAISSLRRKSEGASGDS"
BASE COUNT          562 a         1022 c         1100 g          399 t
ORIGIN      
        1 gaagacgccc ggatcgagga agtgctcggt cagccggatg cggtcgccga gcagcgcgcc
       61 ctgcgtgaac ggcgacgagg gatcgatcga cagcaccgcc acctcgcggt ccttcgagcg
      121 cgcctccttg acgagcgcgc ctatgagcgt cgacttgccc acaccgggcg ggccagtgaa
      181 gccgaccatc gccgcgttgc ccgtgcgcgg gtagacctcg cgcaccagcg cccagccctc
      241 gggatcgtcg ttctccacga gcgagatcgc gcgcgcgagc gcgcgcttgt ctcccgcgag
      301 caactgctcg ctcagctccg ccgctgtgcg tccgggggcc gccatgggcg gcgaatccta
      361 cggcgcaccg cggcaagccg aattttggaa tcgtattcta gaatctcgtt ctatcgccgc
      421 tcccaccgcc cgctccagct caaccgccgc gcgccgccac gcggtgctcg acgcggcgct
      481 cgacgccttc gacgaacacg gattcagcgg cgcgagcatc gaggaggtgc ggcaccgctc
      541 gggggcgagc atcggctcga tctaccacca cttcggcggt aaggagggcc tcgcggcgga
      601 gctgtacgtc gggggactgc gctcctacca ggaaggcctc ctcgaggcgc tcgagcagag
      661 cgcgagcgcg caggccggcg tggaggcagc ggtccgccac cacctgcgct ggatcggcca
      721 gaacccgaag ctcgcgcgct tcatgttcga gaacagcatc gccacgccgg agcttcgagc
      781 gcagaaccgc cgattcttcg ccgccgtgca cgagtggctc gagcggcagg ccgagcgcgg
      841 cgagatccgc cggctgccgg gcgacctcta ctacgcgctg ctcatcggcc cgagccagga
      901 gctcgcccgg cactcgctcg ccggtcggat cgagataccg ctcggggatg cggccgacga
      961 gctcgcgcac gccgcgtgga tgtcacttcg caacgaccaa ggaggaccgt catgaccgtg
     1021 accgtgtacg aggacatcgt cgacgcggcg cgcgccgaca cacgcgagct cgtgcgggtg
     1081 gagcgcgacg gcgaccacgc gatcgtgcgc atggatgacc cggagtcgct caacgcgctg
     1141 tcggctccgc tcacgctcca gctccacgac gcactcgccg ccctggccgc cgaccgggcg
     1201 attcgctcca tcgtgctcac aggcagcgac cccgccttct ccgccggagg cgatctgcgc
     1261 ctgatgcagg acgcgcacga gggcatcgac gagggcgacc agggcgcgac gctcgcgtgg
     1321 cgatggattc ggtaccagtt cggcggaatc gcgcggctga tcacgcgcac ggacaaggcg
     1381 ttcatcgccg cggtgaacgg cgccgcggcc ggcgtcggcc tcgcgttcgc gctcgcctgc
     1441 gacatcgtga tcgcttccga gcgcgcgcgg atcgttcccg cgttcatgaa gatcgggctg
     1501 ctgcccgagg tgggcacgag ctggcacctc acgcgcagga tcggctacca acgcaccttc
     1561 gagctgttcg ccgggggccg gatcctgtcg gcgcaggagg cagacgacct cggcatggtg
     1621 aacgccgtcg tgccgcacga cgcgctgatg gacgcggcgc tcgagtggtg caagcggatc
     1681 tcgcagctgc cgccccacgc gctcgccatg acgaagccgc tgctgcggaa cgccgcggac
     1741 atgccatggg aaggcgcgat cacgatggag gagctagcgg agccgaactg cttcaccacc
     1801 accgcgcacc gcggcgcggt gcaggcgctg ctcgcgcact aaccgaccgc ctatcgacgg
     1861 acatgtttcg gcgtgtacgg ggtgggcatg ctcgctatct cagatcgccg taggaggagt
     1921 ttgaagatgt cgttgtcaga gggtctgcga gagatgccgt ccaacgctgc ttggctactg
     1981 tcgcgagctc gcgaccgacg gcggaaagta agcgcagcca tgctcgacgc cgctcccgtg
     2041 ggaggggact cagtcgagat ccggatgaag cgcgcgcacg atgccgcgga gcgagcccgg
     2101 gaggtggagc agcgcgccgt ggaggccgag caggaggcca aggattactc cgatcacgct
     2161 cgagaggtga gcgagcacgg ccgcgcccgc gtgaccgagg tgaaccggga gacggagcgg
     2221 caggtcaaac agcgggtcgc cgaggctgaa aaagccgcgg ccgaggccgt gaagggcgaa
     2281 cgccgagctg ccgaagcgga tgcagaagag gaacggcaag aagtccgcgg cgaagtcgac
     2341 gaggagatcg aggctgccga gcgtgatgcc gaggcggccc gacagcgagc gcaggagctc
     2401 gtcgcggatg ccacggaaca actggcggac gcgaggcggc tagcggaaga ggccgctgaa
     2461 gctgcacgcg ccgccgcgga ggaggcacac cgccaggcgc agcagcttgc ggacgaagca
     2521 cagcagcaag ccagcgacgc cgaggctcgg gtcaaagctg ccgagcaagt acgcgagcag
     2581 tcagcggggg ccgcgaggca caccgcacgc gagctcgagc gaaatccaac gaacggcggt
     2641 ctacagtcct acaacaagcc ggagctgatc gaactcgcgg ccagcatcgg aatcgaggga
     2701 cggaggagca tgagcaaggg cgagctcgtc gacgcgattg cgaaggcgtc tcgtaggcgc
     2761 tgaaacggag gcggacccac atgcaactgt tcaaccggaa gagcgaactg gagcgcatgc
     2821 tcgaaaaact caccgacccg ctcgaggcga cgagcaagtt gacgttcgac atgccgagcg
     2881 gaggggcggg caaggcactg aaggtgggct tgatcagcgc tggtggactc gccggcctaa
     2941 cggcggcgag cgcagcgatc tcttctctca ggcgaaagag tgagggagcc agcggcgatt
     3001 cgtgacggcc tagggcgcgg tggcgctcac gatccaggta gacaccggcg cgcgcactcc
     3061 atccggtcct gcgtattcgg cga
//
LOCUS       DASVXS010000040         8161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_152341, whole genome shotgun sequence.
ACCESSION   DASVXS010000040 DASVXS010000000
VERSION     DASVXS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152341"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1988
                     /gene="infB"
                     /locus_tag="VF752_02195"
     CDS             <1..1988
                     /gene="infB"
                     /locus_tag="VF752_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX8854384.1"
                     /translation="ARRPERPTRPQRGGPGGPGGPGGGGRRRVVIDSQASRRQQGPPP
                     PQQQQPPRRRGRRRRTPWVEPDLTAPPEAPKEEPATQINSGATVKEVAESLGLGAPEV
                     IKKLMELGEMATLTQTLSDEAIGVLADEFDKKIEIVHAADDVVAEPEVVEDESELVER
                     PPVIVIMGHVDHGKTSLLDAIRESEVAAGEAGGITQHIGAYQVTHDGHTLTFLDTPGH
                     QAFTAMRARGAQVTDIAVIVVAADDGVMPQTQEAIDHAKAAEVPIMIAVNKIDKEGAD
                     PNRVRGELAQLGLTPADWGGDTEFSDVSAKTKQGLDELLENIVTLAELEELRANPEAE
                     ASGSVIESRLDPGRGPVVSLLIQRGTLAVGDAVVAGPNWARVRAMQDFRGERMKAARP
                     GDPVEILGFDGVPEAGEHFRVVENDRRARQLAGERANRLKTEQLARRQGVRVSLEDVF
                     ARAQKGELKELNLIVKADVAGSLEALEDEIAKLPHDQVTVNVIHDGVGGINESDVMLA
                     AASDAIIVGFNVRPVGDARQVAEREGVEIRTYSVIYRVVEELRQAMEGLLEPEEVEQT
                     IGTAEVRQTFRASRVGTIAGCFVRDGVIRRGARARLVRGGTIVYDGRIDTLRRFQDDV
                     REVEAGYECGVTLENYDDIKEEDVLEVYETRQIEREL"
     gene            1990..2292
                     /locus_tag="VF752_02200"
     CDS             1990..2292
                     /locus_tag="VF752_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX8854385.1"
                     /translation="MSYVVLLEIHLHFPENGSLKGKRRELNSLKAHLRQRFGASVAET
                     DHHELWQRATVSAALVGRDAHTVEDAAAKLERYVLSQYPDGVRFERGLISRDEVLS"
     gene            2371..2721
                     /gene="rbfA"
                     /locus_tag="VF752_02205"
     CDS             2371..2721
                     /gene="rbfA"
                     /locus_tag="VF752_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX8854386.1"
                     /translation="MRRVNEAVREVLSAHIAADLKDPRIGFVTVTYVDTTPDLRHARV
                     YVSVLGGHSERGAAMDGLRSAAGFLQSRVGDELRMKRTPTLEFRYDESIDRGMRISEI
                     LDSELPEKPEGGDE"
     gene            2718..3704
                     /locus_tag="VF752_02210"
     CDS             2718..3704
                     /locus_tag="VF752_02210"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX8854387.1"
                     /translation="MSVGTELDQIIEELRQSDKLLLTTHENPDGDALGSLLATHMILR
                     LLGKDVVMFMSSNEFPLPWEYRDMPFDGVLHEPPADADERTIVFLDCGNIDRMPVDWL
                     RQDHLHILNIDHHHDNTRFGTVNLVDPDASCTAEIVWRIAKELDVEITPPIADALYVG
                     LVTDTGRFMYENTTPEAHRMAADLIEAGVEPHDVYRRLYEDLPFARLQLLARALARVR
                     RFYEGALTLTYLTREDYEETGSIETDSEGVVDHIRAVEGTAVAALVRDLLSDDREGMR
                     KVSLRSTDGRVDVSRIARSHGGGGHRQAAGFSTELSIEELVDSLRREVAEQL"
     gene            3862..4668
                     /gene="truB"
                     /locus_tag="VF752_02215"
     CDS             3862..4668
                     /gene="truB"
                     /locus_tag="VF752_02215"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX8854388.1"
                     /translation="MIDHGVILFPKPAGKTSHDIVAGVRRTLSERLGRRVKVGHAGTL
                     DPFATGLLLVLVGTATRAQRFLLGLSKTYRAVARLGWTSTTGDRDGEVTHTGKVPEEL
                     DLPTGEMMQRPHAYSAVKVGGERLYKKARRGEEASAEPRPITVYRFEQIWREGDRVGL
                     EIECSAGTYVRQLVADLGDAYCEELERTAIGPFRLEDADPDRVVPLSEALSFLPERPL
                     DDEEAAKVANGVAVEGEATNGFVRLTHGGDVVAVAEPRDHSLRPRVVFPQ"
     gene            4665..5549
                     /locus_tag="VF752_02220"
     CDS             4665..5549
                     /locus_tag="VF752_02220"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX8854389.1"
                     /translation="MKVTGLWDAERRPRKVAVGTFDGVHLGHREVIRGADTVLTFRPH
                     PVAVTRPEALPKLIYPFEIKRDLIDGLGVDELVVIPFDKEFSQRSAENFIEEILIGRL
                     GAEQVSIGENFRFGKGARGDAELLRSYDQFETRVVPLVEVSGETVSSSHIRGLIAAGE
                     VDKASEFLGGPFLFEGEVVPGDKRGRELGIPTANIVPDDRFVSPGHGVYAAWAHGHPS
                     AVNVGVRPTFETGRGLLVEAYLLDFDDDLYGQTLRIAFLERMRGEKRFDSADALVAQM
                     RRDVEQAREICSATVTRR"
     gene            5546..5815
                     /gene="rpsO"
                     /locus_tag="VF752_02225"
     CDS             5546..5815
                     /gene="rpsO"
                     /locus_tag="VF752_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX8854390.1"
                     /translation="MTLTTDEKREIVTRFGKGDNDTGSTEVQVALLTRRINDLTEHLR
                     EHKKDHHSRRGLLMLVGQRRRQLKYLQRKDLDRYRSLIQELGLRR"
     gene            5812..6276
                     /locus_tag="VF752_02230"
     CDS             5812..6276
                     /locus_tag="VF752_02230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8854391.1"
                     /translation="MIAPGTPAPDFALKDQDGNVVSLDDLKGQTTLLVFYPLDFSPVC
                     TDQLNVYQEVLDELRERGVQMYGISVDSAYSHKAFQEKLNVTIPLLSDFEPKGQVARS
                     FGVYADDYGVAQRALVLIGPDGVVKWSHVSPSPLEVPGVNLIFDALDAAPAA"
     gene            6285..6797
                     /locus_tag="VF752_02235"
     CDS             6285..6797
                     /locus_tag="VF752_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8854392.1"
                     /translation="MADSLSSAQPPAPSAEDHVRGESGLLVIEYGDYECPYCAKTEML
                     LADLPLRRVFRHFPVPSKHPRAPALSHAAEAVALQGRFWEMHESLFTDQGHLDDPHLW
                     NRIRDLGLDLDRFEADRRSDRVRERVERDFRSGLRAGVMTTPTLFVNGEAYAGVPDAE
                     LLTRLRAGPG"
     gene            complement(6794..7468)
                     /locus_tag="VF752_02240"
     CDS             complement(6794..7468)
                     /locus_tag="VF752_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854393.1"
                     /translation="MRRVLITLAALGGALTVAAPAAAATPLDEFGIVAYEIRSASGYE
                     SVSFHGDGTDSCRKAGLCDVSGQARYEFRSRGRGFAGLFGLGTPDVGGFGGFSVSGTT
                     TSSVSQTGASAACTDFVKHDEDSFTMEPKGRRILFGFHLDPYQPDYLRTHCAGPGESD
                     LALARKLPQAIVSPKELVKRRLTVTLVRNGPFHVGAFSGTVDSRIVIVLRQSKRALDL
                     LDLFDS"
     gene            complement(7478..8071)
                     /locus_tag="VF752_02245"
     CDS             complement(7478..8071)
                     /locus_tag="VF752_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854394.1"
                     /translation="MAASRAAFCAAALASAVLIPAAPAVAKSERYSVTYARGLVQMSF
                     QGNGTSGLVKHTIRSAKRRGAVLYDPSNGKGSGSFPVDARTVAESDSCTDNVKVRRER
                     IDLLPRSNRREQIVFAPAPDRAHDVLATKCAAPGLSDLDAAQIPEVVVPRAPFTNDTL
                     RLTMRFSAHFQAGAYSGRVQMRLRLVMRRSTRDASGR"
BASE COUNT         1332 a         2769 c         2873 g         1187 t
ORIGIN      
        1 gcgcccggcg tcccgagcgc cccacgcggc cgcagcgcgg cggccctggc ggtccgggtg
       61 gccccggcgg cgggggacgc cgtcgcgtgg tcatcgactc ccaggcttcg cggcggcagc
      121 agggtccgcc gcctccgcag cagcagcagc cgccccgccg ccgcgggcgc aggcgccgca
      181 cgccgtgggt cgagcccgac ctgaccgcgc cgcccgaggc gccgaaggaa gagccggcaa
      241 cgcagatcaa ctccggcgcg acggtcaagg aggtcgctga gtcgctcggg ctcggcgcgc
      301 ccgaggtgat caagaagctg atggagctcg gcgagatggc cacgctcacg cagacgctct
      361 ccgacgaggc gatcggcgtg ctggccgacg agttcgacaa gaagatcgag atcgtgcacg
      421 cggccgacga cgtggtcgcg gagccggagg tggtcgagga cgagagcgag ctcgtcgagc
      481 gcccgccggt gatcgtgatc atgggccacg tcgaccacgg caagacgtcg ctgctggacg
      541 caatccgcga gtccgaggtg gcggccggcg aggcaggcgg catcacgcag cacatcggcg
      601 cctaccaggt cacgcacgac ggccacacgc tcacgttcct cgacacgccg ggccaccagg
      661 cgttcaccgc catgcgcgct cgcggcgcgc aggtcaccga catcgcggtg atcgtggtcg
      721 cggccgacga cggcgtgatg ccgcagacgc aggaggcgat cgaccacgcg aaggcggccg
      781 aggtgccgat catgatcgcg gtcaacaaga tcgacaagga aggcgccgac ccgaaccgcg
      841 tgcgtggcga gctcgcgcag ctcggcctca cgccggcgga ctggggcggc gacaccgagt
      901 tctcggacgt ctccgcaaag accaagcagg gcctcgacga gctgctcgag aacatcgtca
      961 cgctcgccga gctcgaggag ctgcgcgcca acccggaggc tgaggcgtcg ggcagcgtga
     1021 tcgagtcgcg gctcgacccg ggccgcggtc ccgtggtatc gctcctgatc cagcgcggca
     1081 cgctcgcggt gggcgacgcc gtcgtggcgg gccccaactg ggcgcgcgtg cgcgcgatgc
     1141 aggacttccg cggcgagcgc atgaaggccg cgaggccggg cgacccggtc gagatccttg
     1201 gcttcgacgg cgtgcccgag gccggcgagc acttccgcgt ggtggagaac gaccgccgcg
     1261 cccgccagct cgcgggtgag cgtgcgaacc gcctgaagac cgagcagctc gcgcgccgcc
     1321 agggcgtgcg tgtgtcgctc gaggacgtct tcgcccgtgc ccagaagggc gagctcaagg
     1381 agctgaacct gatcgtgaag gccgacgtgg ccggctcgct cgaggcgctc gaggacgaga
     1441 tcgccaagct gccgcacgac caggtgaccg tgaacgtgat ccacgacggc gtgggcggca
     1501 tcaacgagtc cgacgtgatg ctggccgccg cctccgacgc gatcatcgtg ggcttcaacg
     1561 tccgcccggt cggcgacgct cgccaggtcg ccgagcgcga gggcgtcgag atccgcacct
     1621 actcggtgat ctaccgcgtg gtggaagagc tccgccaggc gatggagggt ctgctcgagc
     1681 ccgaagaggt cgagcagacg atcggcaccg ccgaggtgcg ccagaccttc cgcgcgtcgc
     1741 gcgtgggcac gatcgctggc tgcttcgtgc gcgacggcgt gatccgtcgc ggcgcgcgcg
     1801 cccgtctcgt gcgcggcggc acgatcgtct acgacggccg gatcgacacg ctccggcggt
     1861 tccaagacga cgtgcgcgag gtagaggccg gctacgagtg cggcgtcacg ctcgagaact
     1921 acgacgacat caaggaagag gacgtgctgg aggtctacga gacccgccag atcgagcgcg
     1981 aactctgatt tgagctacgt cgtcctactc gagatccacc tgcacttccc cgaaaacgga
     2041 agcctcaagg gaaagcgtcg cgagctcaac tcgctcaagg cgcatctgcg ccagcgcttc
     2101 ggcgcgagcg tcgccgagac tgaccatcac gaactgtggc agcgggccac ggtctcggct
     2161 gcgctcgtgg gccgcgacgc ccacaccgtc gaggacgcgg cggcgaagct cgagcggtac
     2221 gtgctgtcgc aataccccga cggagtgcgg ttcgagcggg ggctgatctc ccgggatgag
     2281 gtgctctcgt gactggccgt tcagtcggaa atcaattgac aacgttgacc tgtgacgggg
     2341 gcgtcattgt ctaggatccc ttccggccgc atgagaaggg tgaacgaggc ggtgagagaa
     2401 gttctctcag cacacatcgc ggccgacctc aaggaccctc ggatcggatt cgtgaccgtg
     2461 acgtacgtcg ataccacccc agatttgcgc catgcgcgcg tttatgtgag cgtgctcggt
     2521 gggcactccg aacggggggc cgcgatggat gggctccgct ccgcggcggg cttcctccag
     2581 tcgcgcgtag gagacgaact acgtatgaag cgaactccca cgcttgagtt ccgctacgac
     2641 gagtcgatag accgggggat gcggatctcg gagattctcg actccgagct gccggagaag
     2701 cccgaagggg gcgacgagtg agcgtcggca ctgagctcga ccagatcatc gaggagctgc
     2761 gccagtcgga caagctcctc ctcacgacgc acgagaaccc cgacggcgac gccctcggct
     2821 cactgctcgc tacgcacatg attctgcgcc tgctcggcaa ggacgtcgtg atgttcatgt
     2881 cgtcgaacga gttcccgctg ccctgggagt accgcgacat gccattcgac ggtgtgctcc
     2941 acgagccacc ggcggacgcc gacgagcgca cgatcgtgtt cctcgactgc ggcaacatcg
     3001 accggatgcc ggtcgactgg ctgcgccagg accacctgca catcctcaac atcgaccacc
     3061 accacgacaa cacccgcttc ggcacggtga acctcgtgga cccggacgcg tcgtgcaccg
     3121 ccgagatcgt ctggcggatc gcgaaggagc tcgacgtgga gatcacgccg ccgatcgccg
     3181 atgcgctcta cgtcgggctg gtcaccgaca ccggccgctt catgtacgag aacacgacgc
     3241 ccgaggcgca ccgcatggcc gccgacctga tcgaggcggg ggtcgagccg cacgacgtgt
     3301 acaggcggct ttacgaggac ctgccgttcg cgcggctgca gctgctggca cgggcgctcg
     3361 cgcgggtgcg gcggttctac gagggtgcgc tcacccttac ctatctcacg cgcgaggact
     3421 acgaggagac gggctcgatc gagaccgact ccgagggagt tgtcgaccac atccgcgcgg
     3481 tggaggggac ggcggtggcg gcgctcgtcc gcgatctgct ctccgacgac cgcgagggca
     3541 tgcggaaagt gagcctgcgc tcgacagacg gtcgcgtgga tgtgtcgcgg atcgcgcggt
     3601 cgcacggcgg cggggggcac cgccaggcgg cggggttctc caccgagctg tcgatcgaag
     3661 agctcgtgga ctcgctgcgg cgcgaggttg ctgagcagct gtaaacggca gcatttaggg
     3721 gccgagggag gttgagtggg ctcgctgagc ccaggggcgc tgggggtggg ccgttttgac
     3781 catgactatc aatgtcaaaa cggcccaggt ccgttctcgc cgagagtggc cagccgccgg
     3841 cgctcggtag gctcccgcgc cgtgatcgac cacggagtga tcctctttcc caagcccgcc
     3901 ggcaagacct cgcacgacat cgtcgctggg gttcggcgaa cgctgagcga gaggctcggg
     3961 aggagggtga aggtcggcca cgccggcacg ctcgatccct tcgccaccgg cctcctgctc
     4021 gtgctggttg gcaccgccac ccgcgcccag cgcttcctct tggggctgtc gaagacctac
     4081 cgcgccgtgg cgcgcctcgg ctggacgtcc acgacgggcg accgcgacgg cgaggtcacg
     4141 cacaccggca aggtgcccga agagctcgat ctcccgaccg gcgagatgat gcagcgcccg
     4201 cacgcctact cggcggtcaa ggtaggcggc gaacgcctct acaagaaggc gcggcgtggg
     4261 gaggaggctt cagccgagcc ccggccgatc acggtttacc gcttcgagca gatctggcga
     4321 gagggcgacc gcgtgggtct cgagatcgaa tgctccgccg gcacctacgt gcgccagctc
     4381 gtggccgacc tcggcgacgc ctactgtgag gagctcgaac gcaccgcgat cgggccgttc
     4441 cgcctcgagg acgccgatcc cgaccgcgtc gtccccctca gcgaggcgct ctcgttcctg
     4501 cccgagcgcc cgctcgacga cgaggaggcc gcgaaggtgg ccaacggcgt ggcggtagaa
     4561 ggggaggcga ccaacggctt cgtccgcctc acgcacggcg gagacgtcgt ggccgtcgcg
     4621 gagccgcgcg accactcgct gcgtccccgg gtggtgttcc cgcagtgaag gtcacagggc
     4681 tttgggacgc cgagcggcgg ccccgaaaag tcgcggttgg cacgttcgac ggggtgcacc
     4741 tcggccaccg cgaagtgatc cgcggggcgg acaccgtgct cacctttcgc ccgcacccgg
     4801 tcgcggtcac gcggccggag gcgctgccga agctgatcta cccgttcgag atcaagcgcg
     4861 acctgatcga cggcctcggc gtggacgaac tcgtggtcat cccgttcgac aaggagttct
     4921 cgcagcgctc agccgagaac ttcatcgagg agatcctgat cggccgccta ggcgccgagc
     4981 aggtctccat cggcgagaac ttccgcttcg gcaagggcgc caggggcgat gcggagctgc
     5041 tccgcagcta cgaccagttc gagacgcgcg tggtgccgct cgtcgaggtg tcgggtgaga
     5101 ccgtgtcctc gagccacatc cgcggcctga tcgcggcagg tgaggtggac aaggcctccg
     5161 agttcctggg cggcccgttc ctgttcgagg gggaggtcgt gcccggcgac aagcgcggac
     5221 gcgagctcgg cataccgacc gccaacatcg tcccggacga ccgcttcgtg agcccgggcc
     5281 acggcgtcta cgcggcctgg gcgcacgggc acccatcggc ggtcaacgtg ggcgtgcgac
     5341 cgaccttcga gaccgggcgc ggcctgctcg tcgaggccta cctgctcgac ttcgacgacg
     5401 acctctacgg ccagaccctg cgtatcgctt tcctcgagcg gatgaggggc gagaagcgct
     5461 tcgactcggc cgacgcgctg gtggcccaaa tgcgccgtga cgtggagcag gcgcgcgaga
     5521 tctgctctgc tacggtaacg cgccgatgac tctgaccact gacgagaagc gagagatcgt
     5581 cacccgcttc gggaagggcg acaacgacac gggctccacc gaggtccagg tggccctgct
     5641 cacacggcgc atcaacgatc tcacggagca tctccgcgag cacaagaagg accatcactc
     5701 ccgccgcggg ctcctgatgc tcgtcggtca gcgccggcga cagctcaagt acctgcagcg
     5761 caaggacctg gatcgctacc gctcgctgat ccaggagctc ggcctgcggc gatgatcgcg
     5821 cccggcactc ccgccccgga cttcgcgctg aaggaccagg acggcaatgt ggtgtcgctc
     5881 gacgatctga agggccagac cacgctgctg gtcttctacc cgctcgactt cagcccggtc
     5941 tgcacggacc agctcaacgt gtaccaggag gtgctcgacg agctgcgcga gcgcggcgtg
     6001 cagatgtacg gcatctccgt cgactccgcc tactcgcaca aggccttcca ggagaagctg
     6061 aacgtcacga ttccgcttct ctccgacttc gagccgaagg gccaggtggc gcgcagcttc
     6121 ggcgtctacg cggacgacta cggggtcgcc cagcgcgcgc tcgtcctcat cggacccgac
     6181 ggcgtcgtga agtggtcgca cgtctcgccg agcccgctcg aggtcccggg cgtgaacctg
     6241 atcttcgacg cgctcgacgc ggcccccgcc gcctaggcgg gcccgtggcg gacagcctct
     6301 cgagcgccca gccgccggcg ccgagcgccg aggaccacgt ccgtggtgag tcgggtctgc
     6361 tcgtgatcga gtacggcgac tacgagtgcc cctattgcgc gaagaccgag atgctgctcg
     6421 cggacctgcc gctgcggcgg gtgttccggc acttcccagt gccgtcgaag cacccgcgcg
     6481 caccggcgct ctcgcacgcg gccgaggcgg tcgcgctcca ggggcgcttc tgggagatgc
     6541 acgagtcgct cttcaccgac cagggccacc tcgacgaccc gcacctgtgg aaccgcatac
     6601 gcgacctcgg gctggacctc gaccgtttcg aggcggaccg ccgctcggac cgcgtgcgcg
     6661 agcgcgtcga gcgcgacttt cgctcggggc tcagggcggg cgtgatgacc acgcccacgc
     6721 tgttcgtgaa cggcgaggcc tacgcgggcg tgccggacgc cgagctgctg acccggctgc
     6781 gcgcgggccc gggctaggag tcgaagaggt cgagcaggtc gagcgcgcgc ttcgactggc
     6841 gcagcacgat cacgatgcgc gagtcgaccg tccccgagaa ggcgcccaca tggaaggggc
     6901 cattgcgcac gagcgtgacg gtgagccggc gcttgacgag ctccttaggc gagacgatcg
     6961 cctgcggcag cttgcgtgcg agcgccagat cgctctcccc gggcccggcg cagtgcgtgc
     7021 gcaggtagtc cggctgatag gggtccaggt ggaagccgaa caggatgcga cggcccttcg
     7081 gctccatcgt gaagctgtcc tcgtcgtgct tcacgaagtc agtgcacgcc gccgacgcgc
     7141 cggtctggct gacggacgag gtggtggtgc cgctgacgct gaacccgccg aagccgccca
     7201 cgtccggggt ccctagcccg aacagcccgg caaagccgcg accgcgcgag cggaactcgt
     7261 accgggcctg cccactcacg tcacagagcc cggccttgcg gcacgagtcg gtgccgtctc
     7321 cgtggaagct cacgctctcg tagccgctcg cgctgcggat ttcgtacgcg acgatcccga
     7381 actcatccag cggagtcgcg gcagcggccg gcgcggcgac cgtcagcgcg cccccaagag
     7441 ccgcgagcgt gatgagaacc cggcgcatct accggggtta tcggcctgac gcgtcgcgag
     7501 tggagcgtcg catcacgagc cggaggcgca tctgcacccg cccggagtag gcgcccgcct
     7561 ggaagtgagc gctgaagcgc atggtcagcc ggagcgtgtc gttggtgaag ggcgcgcgcg
     7621 gcacgacgac ctcgggtatc tgcgccgcgt cgagatcgga caagcccgga gccgcgcact
     7681 tcgtggccag tacgtcatgg gcgcggtcgg gcgccggcgc gaacacgatc tgctcgcgcc
     7741 ggttcgaccg cggcaggagg tcgatccgct cacgtctgac cttcacgttg tccgtgcatg
     7801 agtcgctctc ggcgacggtg cgtgcgtcga ccgggaagct ccccgatccc ttgccgttgc
     7861 tcgggtcgta cagcactgcg ccgcggcgtt tggcgctcct gattgtgtgc ttcacgaggc
     7921 cgctcgtgcc gttgccctgg aagctcatct gcacaaggcc gcgagcgtac gtgaccgagt
     7981 aacgctcgga cttggccacc gccggcgcag cgggaatgag gaccgcagac gcgagggcgg
     8041 cggcgcagaa cgcggccctg gaagctgcca tgtaggcacg actctgtgcc gaaaaggtgt
     8101 ggttgtcaac acttgtgggg gttcgttgag cgctctacgt tgaccgcgat ggccggtcca
     8161 c
//
LOCUS       DASVXS010000041         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_152367, whole genome shotgun sequence.
ACCESSION   DASVXS010000041 DASVXS010000000
VERSION     DASVXS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152367"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /gene="trpA"
                     /locus_tag="VF752_02250"
     CDS             complement(<1..415)
                     /gene="trpA"
                     /locus_tag="VF752_02250"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX8854395.1"
                     /translation="MADVSAPAGAERIAAAFTTHGKRAALMPYLMGGFPSIDKSLAVG
                     EACADAGADLVELGVPFSDPLADGPVIHAAATEALAAGATVDGVLGVCESLSARLPVV
                     LMIYANPVLQRGAEAFVKRAAGAGAAGLIVPDLPHD"
     gene            complement(408..1580)
                     /gene="trpB"
                     /locus_tag="VF752_02255"
     CDS             complement(408..1580)
                     /gene="trpB"
                     /locus_tag="VF752_02255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX8854396.1"
                     /translation="MSAGKVEPGVEERFGPYGGRYVPETLIPALDELEQAWLEAREDP
                     AYHADLGRLLRDYAGRPTPLYLAERLSSEVGHPVYLKREDLLHTGAHKINNALGQALL
                     ARRMGKTRVIAETGAGQHGVATATACALLGIDCLVYMGTEDMRRQRPNVERMNLLGAT
                     VEPVDAGARTLKEATSAAIRDWVASVGHTHYVIGSAVGPSPYPALVRDLQRVIGDEMR
                     AQLLEAEGRLPQRVIACVGGGSNAIGSFVPFYDDDVELVGVEAAGEGIESGRHGAPLT
                     TGARGILHGSLSAVLQDDEGQILEAHSISAGLDYPGSGPEHAFLRDSRRARYEAVTDE
                     QALEAFRLLARLEGIIPALESAHALAWLLADRESELDALTLSGRGDKDLDEVLERG"
     gene            complement(1577..2215)
                     /locus_tag="VF752_02260"
     CDS             complement(1577..2215)
                     /locus_tag="VF752_02260"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX8854397.1"
                     /translation="MTRVKICGVTQLEDARAAAELGAWAIGMILWEGSERRCDIAAAE
                     EIGAEMKRHVEVAGVFVNATLDEVAYAADRCSLTMVQLHGDEGPAFCREAARRTGAKV
                     IKAARVKDAASIQALSAFHTDFHLLDAHVPGRRGGTGETFQWDLVRLHGREVPVILSG
                     GLTPDNVGEAIEATHPFAVDTASGTEASPGVKDHARMLAFFRAVQHADRVPA"
     gene            complement(2297..3529)
                     /locus_tag="VF752_02265"
     CDS             complement(2297..3529)
                     /locus_tag="VF752_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8854398.1"
                     /translation="MRRNVLSGVAGGLLVLVVGSILVATDVIDTGDTTKTVVQQASLP
                     GPTTPASSKRAAGLTVENIYKQDGPGVVFIQSRIVEQTQSPFGFPQAQQGVATGSGFV
                     LDKKGYILTNAHVVEGAKQIGVRFGNGSLIDAKLVGTDPSTDLAVLKVDPGEGTLVPL
                     NLGDSSKVNVGDPAIAIGNPFGYDRTVTTGIISALQRQIQAPNGFKINNVLQTDAAIN
                     PGNSGGPLLDGAGRVIGINSQIATGGSGNGNVGIGFAIPINTAKAVIPQLIGAGKASH
                     AYLGISTAGISAQEAKDLNLPTSKGALVQQVVKGGPAAKAGLKAGDSQTSSGLVIGGD
                     LIVKVDSTTIAKPDDIAGAIASHKPGDQVTIHFYRGTKLHTAKITLGNRPSGFDQSQQ
                     SQGGQGGSGQLPFPFPSP"
BASE COUNT          510 a         1336 c         1305 g          577 t
ORIGIN      
        1 cgtcgtgcgg caggtcgggc acgatcagcc cggccgcacc cgcgcccgcc gcccgcttca
       61 cgaaggcctc ggcccctcgc tgcagcaccg ggttcgcgta gatcatcagg acgaccggca
      121 accgggcgga gagcgactcg cacacgccga gcaccccgtc gaccgtcgcg cccgcggcga
      181 gcgcctccgt cgcggcggcg tggatcaccg gcccatccgc gagcgggtcg gagaacggca
      241 ctccgagctc gacgagatcg gcgccagcgt cggcgcacgc ctcgcccacc gcgagcgact
      301 tgtcgatgct cgggaagccg cccatgagat acggcatcag cgcggcgcgc ttgccgtgcg
      361 tggtgaacgc ggcggctatc cgctcggcgc ccgccggcgc cgacacgtca gccacgctcg
      421 agcacctcgt cgaggtcctt gtcgccacgc ccggagagcg tgagcgcatc gagctccgac
      481 tcccggtcgg cgagcagcca cgcgagcgcg tgcgcggact cgagcgccgg aatgatcccc
      541 tcgagccgcg cgagcagccg gaatgcctcg agcgcctgct cgtccgtgac cgcctcatag
      601 cgtgcgcggc gcgagtcgcg caggaatgcg tgctcggggc ctgagcccgg gtagtcgagg
      661 ccggccgaga tcgagtgggc ctcgagaatc tgaccctcgt cgtcctggag cacggcggac
      721 agcgaaccgt gcaggatgcc gcgcgcgccg gtggtcagcg gcgcgccgtg ccggcccgac
      781 tcgatccctt cgcccgcggc ctccacgccg accagctcga cgtcatcgtc gtagaagggc
      841 acgaagctcc cgatcgcgtt cgagccgccg cccacgcagg cgatcacgcg ctgcggcaac
      901 cggccctcgg cctcgagcag ctgcgcgcgc atctcgtcgc cgatcacgcg ctgaaggtcg
      961 cgcacgagcg cgggatacgg cgagggcccg acggccgagc cgatcacgta gtgcgtgtgg
     1021 ccgacactcg ccacccagtc gcggatcgcg gcgctggtcg cctccttcag cgtgcgcgcc
     1081 ccggcgtcga cgggttcgac tgtcgcaccg agcaggttca tgcgctccac gttcggacgc
     1141 tggcgccgca tgtcctcggt gcccatgtag accaggcagt cgatgccgag cagcgcgcac
     1201 gctgtcgcgg tcgcaacgcc gtgctggcct gcaccggtct ccgcgatcac gcgcgtcttg
     1261 cccatgcggc gggcgagcag cgcctgaccg agcgcgttgt tgatcttgtg cgcccccgtg
     1321 tggagaagat cctcgcgctt cagatatacg gggtggccca cttcggatga gagccgctcc
     1381 gcgagataga gcggggtcgg ccggccggcg tagtcgcgca gcagccgccc cagatccgcg
     1441 tggtacgccg ggtcctcgcg ggcctcgagc caggcctgct cgagctcgtc gagcgcggga
     1501 atcagggtct cgggcacata gcggcccccg taggggccga agcgctcttc gacgccgggc
     1561 tccaccttgc ccgcgctcac gccggcaccc ggtctgcgtg ctgcacggcg cggaagaagg
     1621 cgagcatgcg cgcgtggtcc ttcacgcccg gcgacgcctc ggtgccgcta gcggtgtcga
     1681 ccgcgaacgg gtgggtcgcc tcgatcgcct cccccacgtt gtcgggcgtc aggccgcccg
     1741 agaggatcac cggcacctcg cggccgtgaa gccgcacgag gtcccactgg aacgtctcac
     1801 ccgtgccgcc gcggcggccc gggacgtgcg cgtcgagcag gtggaagtcg gtgtggaagg
     1861 cggagagtgc ctggatcgac gcggcgtcct tcacgcgcgc ggccttgatc accttggcgc
     1921 cggtgcggcg ggcggcctcg cggcagaacg ccggtccctc gtcaccgtgc agctggacca
     1981 tcgtgagcga gcagcggtcg gcagcgtacg ccacctcgtc gagcgtcgcg ttcacgaaca
     2041 cgcccgccac ctcgacgtgc cgcttcatct cggcgccgat ctcctcggct gcggcgatgt
     2101 cgcaacggcg ctcgctgccc tcccagagga tcatcccgat cgcccaggcg ccgagctcgg
     2161 ccgcggcgcg cgcgtcctcg agctgcgtga ctccgcagat cttcaccctg gtcacgcgcc
     2221 aatggtggca tggaagtagc gccgcgtccc gaaggacgcg acgcttggcc ctggcgactc
     2281 actccgctgg gactcgctac ggcgagggga acgggaaggg gagttgcccg ctcccccctt
     2341 gaccgccctg gctctgctgg ctctgatcga agcccgacgg gcggttgccg agcgtgatct
     2401 tggccgtgtg cagcttggtg ccccggtaga agtggatcgt gacctgatcg ccgggcttgt
     2461 ggctcgcgat cgcgcccgcg atgtcatcgg gcttcgcgat cgtcgtcgag tcgaccttca
     2521 cgatcaggtc gccgcctatc acgaggcccg acgaggtctg gctgtcgccc gccttgaggc
     2581 ccgcctttgc agccgggccg cccttcacga cctgctgcac gagcgcgcct ttcgaggtcg
     2641 gcaggttcag gtccttggcc tcctgcgcgg agatccccgc ggtcgagatc ccgaggtatg
     2701 cgtgcgaggc cttgcccgcg ccgatcagct gggggatcac cgccttcgcg gtgttgatcg
     2761 ggatcgcgaa gccgatgccc acgttgccgt tgccgctgcc gcccgtcgcg atctgcgagt
     2821 tgatgccgat cacgcgaccg gcgccgtcga gcagcggacc gcctgagttg cccgggttga
     2881 tcgcggcgtc ggtctgcagg acgttgttga tcttgaagcc gttcggcgcc tggatctggc
     2941 gctggagcgc ggagatgatc ccggtcgtga cggtgcggtc gtagccgaac gggttgccga
     3001 tcgcgatcgc cgggtctccc acgttcacct tgctcgagtc gccgaggttg agcggcacga
     3061 gcgtcccctc gcccgggtcg accttgagca ctgccagatc agtcgagggg tcggtgccga
     3121 cgagcttcgc gtcgatcagg ctcccattgc cgaagcgcac gccgatctgc ttcgcgccct
     3181 cgaccacatg cgcgttcgtg aggatgtagc ccttcttgtc gagcacgaat ccggagccgg
     3241 tcgcgacgcc ctgctgcgcc tgcgggaacc cgaacgggga ctgcgtctgc tcgacgatcc
     3301 gcgactggat gaagacgacg cccgggccgt cctgcttgta gatgttctcg acggtgaggc
     3361 cggcggctcg cttcgagctg gcgggcgtgg tgggcccggg aagacttgcc tgctgcacca
     3421 cggttttcgt ggtgtcgccg gtgtcgatca catcggtggc gacgaggatc gagccgacca
     3481 ccagcacgag caagcctccg gccacacctg agagcacgtt cctgcgcatg cgccgagtct
     3541 cggcggatct gctgaatcgg ttctaaagcg actcgaaaac tgacctaaga gctacgcgcg
     3601 tcggggcttt cgcccgagcc cgggcggcag cgcgagcagc gcgcggatcc tgtgcaacgg
     3661 cccgcctccg cggccgagcg cccgccggag cgccttgcgg tctgcgcgcg tggggcccgc
     3721 gcccggga
//
LOCUS       DASVXS010000042         6282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_365222, whole genome shotgun sequence.
ACCESSION   DASVXS010000042 DASVXS010000000
VERSION     DASVXS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365222"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF752_02270"
     CDS             complement(<1..259)
                     /locus_tag="VF752_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854399.1"
                     /translation="MRGRLGFGLVVLAVGGTAVALAASGGDGVIGPSNHIQPSGRKLE
                     PVGKLTKLGNLPAGGALTVDGRFAWTLSAGRGKNDIRIVRVK"
     gene            494..2053
                     /locus_tag="VF752_02275"
     CDS             494..2053
                     /locus_tag="VF752_02275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX8854400.1"
                     /translation="MLAVTRLVRSERDLSSLLDAITRTITSSFGFRTAVISLYRPAWD
                     DFQVTAVHGSDGARDALMGAVRTWENWAPLLDERFHHAGAYLILHGEFDWSVVGSHSY
                     TPDLEPVDDPRAWHAEDALIVPLMHTDGHYLGMISVDEPHDGLRPGDEELEVLAAVAA
                     HAGLAIQSVQERENAARYQAALEQLLRVSARLSETLSLDSILGAVCEGIQSALGFGKV
                     SVDLVDAEAGLVRPRAAAGWDTNDADLASPVERVKALFDPQFEIQGCFLLPNEEALAR
                     MGLTRPGYESQLNGRGPHAWHHHWLVVPLRDRDGELRAVIWADEPYDRMLPARQRLQA
                     LRMFANQAWTALDSVAHVEELRYLADHDPLTRLGNRRAFMTRLEEEVRRSDRYGTRFT
                     LVVCDLDGFKDVNDRYGHLAGDLALTRMANVLRDTVRGTDGAYRLGGDEFGLILIEAE
                     RDDAVDVATRISETLASDTESSLGEIHASFGVAVYPDEHRTPEALFHAADAQMYAAKR
                     AGGGIGEPVGA"
     gene            2127..3836
                     /locus_tag="VF752_02280"
     CDS             2127..3836
                     /locus_tag="VF752_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854401.1"
                     /translation="MCRSAALSARLASGRWLTVVTGLAAAAPVIAATLRALHDGWMPV
                     GDQGIIATRAYDVFSSHMPLLGQYSEASVSAGHAIYSPGPMLYWLLALPAYSGVTATL
                     TLTIATMNCAAIVGSVALARRRGGLALMLAAAIAVALLSRSFSPESLHDLFNPSAALF
                     PLVLLIFLCWSLACGEYRLLPLTALVASFVAQAHLAYVAPVAGALAVGLVGLALSRTR
                     LARLATPPAAGRRSLLVWSLAAVAVTLVCWSAPIADQIEHHPGNLSLIAQRATASGPT
                     QGAQKGRWALERAVGVPPRWLRSPTRGSGQLALVSGGDYGDTRLSDVSQAPSAGQTGS
                     AIALLAALLVVIALAAWRRRGDLVAGAAIAVVLCVALVEDVAKTPITASNTLGYSIWW
                     GSIVGMWAWLILGWSAWSLLTETVPRGWRLRVPAMARALGLAAVAAVATALALGQGAD
                     THAPYYRPLSAVAGALDRAIPPQTTVGLVQRGGVAVPIEPAIRYTLRIHGVRALGAFA
                     SRRPGAWYELDHRRVNSWVSVNGDRPSRFRPARVVARAVLTDPQGSHRVTATLSPPRA
                     VRV"
     gene            complement(3833..4588)
                     /locus_tag="VF752_02285"
     CDS             complement(3833..4588)
                     /locus_tag="VF752_02285"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX8854402.1"
                     /translation="MTSAGQHRVARVHVPRAPRGTRLPLVVAFHGAGATGQFMENYSG
                     LSGLADQKRFVVAYPSAGGSHPFWSLNETYPNGREDVRFVSDLIDTLRKRLCVDSNRV
                     YATGVSNGGGFTARIGCGLSERFAAIAPVGGGYRAIPDCDADRPLSVLEIHGTSDNAV
                     PYFGKPPDYRGSVPAYVQGWRQRDACAPSTAARRLAYATVLVAWSKCKAGTQVAQIKR
                     YGRGHEWPGAFGAPGFSATAAVWDFFKDKVRAR"
     gene            complement(4755..5930)
                     /locus_tag="VF752_02290"
     CDS             complement(4755..5930)
                     /locus_tag="VF752_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8854403.1"
                     /translation="MSVLPTPAVRQPAPPFTEEHERFREVTRAFVETELRPHAGEWED
                     ARWFPNEVFEKLAEHGYLGLKYPEEYGGQGGDYLHDAVFNEELSRCGSGGLAAGIGAH
                     IGIATPPIWKFGTEDQKQRFLAPAIRGDKIAALGITEPDAGSDVAGIRTFAKKVDGGY
                     VVNGSKTYITNGVRADFVVTAVKTTREGGHHGLSFLVIEKGMEGFSVSRKLEKMGWHA
                     SDTGELAFQDVFVPDENLLGQENRGFYLIMANFQWERLLMALGAVGSMQVTLDRTIEF
                     ALERQAFGRPIAKHQAIRHKLAEMAVKLETGRAMTYNALRLFAEGHDAVQEVTMAKLK
                     TQRDAFDVADEALQIHGGAGYMKEYEIERAARDTRLGPIGGGTDEIMKEILGKTLGL"
     gene            complement(5927..>6282)
                     /locus_tag="VF752_02295"
     CDS             complement(5927..>6282)
                     /locus_tag="VF752_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854404.1"
                     /translation="QEVASFFTDDAVHYYTRLGPHAGARTIGEHTQLAVEHLEGRWTI
                     ENGIEDGEQAVIEWTMTWRDPKSGDRRLDRGTEWFRFRDGRISEVRAYHHSNEKNRSG
                     DLLGFDHAGRGYTTL"
BASE COUNT          841 a         2232 c         2181 g         1028 t
ORIGIN      
        1 ccttcacgcg gacgatccgg atgtcgttct tgccgcgacc ggccgagagc gtccaggcga
       61 agcgcccgtc gaccgtgagc gcgccaccgg ccggcaggtt gccgagcttg gtcagcttgc
      121 cgaccggctc gagcttgcga ccgctcggct ggatgtggtt ggacggcccg atcacgccgt
      181 caccgccgct cgcggcaagt gcgacagcag tgcccccaac agcgaggacc acgagcccaa
      241 atccgaggcg cccacgcatt ccggggcggg aacctagtgg agcgcgccgc aacttgcgac
      301 ggtaacaaca gctttttcac gagtggtggg ctcgcgccaa ccattggccg ggatccgccc
      361 atacgctcaa gcgccccgtt cagcacgtcg atctacctcg ggcgagcccg tctcgtcgcg
      421 agcccgttca gacctgatga aacgccacga ttcctcgccc agcgcccccc cggcggtcga
      481 tcgcctgcac ggcttgctcg cggtcacgcg actggtgcgc agcgagcgcg acctatcgtc
      541 gctgctcgac gcgatcacgc gcacgatcac gagctccttc ggcttccgca cggcggtgat
      601 ctcgctctac cgcccggcct gggacgactt ccaggtgacc gcggtgcacg gatccgacgg
      661 ggcgcgcgac gcgctcatgg gagccgtccg cacgtgggag aactgggcgc cgctgctcga
      721 cgagcgcttc caccacgcgg gcgcctacct gattctgcac ggcgaattcg actggagcgt
      781 cgtcggctcg cacagctaca cgcctgacct cgagccggtg gacgacccgc gcgcgtggca
      841 tgccgaggac gccctgatcg tgccgctcat gcacaccgac gggcactacc tcgggatgat
      901 ctcggtcgat gagccgcacg acggcctgcg gcctggcgac gaggagctcg aggtgctcgc
      961 cgcggtcgcc gcccacgccg ggctcgcgat ccagtccgtg caggagcgcg agaacgccgc
     1021 ccgctatcag gccgcgcttg agcagctgct gcgggtgtcg gcgcgcctca gcgagacgct
     1081 ttcgctcgac tcgatcctcg gagcggtgtg cgagggcatc cagtccgcgc tcggcttcgg
     1141 caaggtgtcc gtcgatctcg tggacgccga ggcggggctc gtccgcccgc gcgccgccgc
     1201 cggctgggac acgaacgacg ccgacctcgc gagcccggtc gagcgcgtga aggcgctgtt
     1261 cgacccgcag ttcgagatcc agggctgctt tctgctgccg aacgaggagg ccctcgctcg
     1321 catgggcctc acgaggccgg gctacgagtc gcagctgaac gggcgcgggc cgcatgcctg
     1381 gcatcaccac tggctggtgg tgccgctgcg cgaccgcgac ggcgagctgc gcgcggtgat
     1441 ctgggccgac gagccctacg accgcatgct tccggcgcgc cagcggctgc aggcgctgcg
     1501 catgtttgcg aaccaggcct ggacggcgct cgactcggtc gcccacgtgg aggagcttcg
     1561 ttacctggcc gaccacgatc cgctcacgcg cctcgggaac aggcgcgcgt tcatgacgcg
     1621 gctcgaggag gaggtgcgcc gttccgaccg ctacggcacg cgcttcacgc tcgtggtctg
     1681 cgacctcgac ggattcaagg acgtgaacga ccgctacggc caccttgccg gcgacctcgc
     1741 gctcactcgc atggccaacg tgctgcgcga cacggtgcgc ggtaccgatg gcgcctaccg
     1801 gctcggcggc gacgagttcg ggctgatcct gatcgaggcc gagcgcgacg acgcggttga
     1861 cgtcgcgacg cgcatctcgg agacgcttgc gagcgacacc gagtcgagcc tcggcgagat
     1921 ccacgccagc ttcggtgtcg cggtgtatcc ggacgagcac cgcacgcccg aggcgctctt
     1981 ccacgcggcc gacgctcaga tgtacgcggc gaagcgcgcc ggcggcggga tcggcgagcc
     2041 ggtcggcgct tagcgccgcc cgccggcctc gatatttctt cgcgagctcc taacgggagt
     2101 aaatgaagcg cgccgtactt tgaaacgtgt gccgctcagc tgcgcttagc gcgcgccttg
     2161 cgtccgggcg ctggctcacg gtcgtcaccg gcctggccgc cgccgcgccg gtgatcgcgg
     2221 ccacgctccg tgcgctgcat gacggatgga tgcccgtggg cgatcagggg atcatcgcca
     2281 cgcgcgcgta cgacgtgttc agctcgcaca tgccgctgct gggccagtac tccgaggcgt
     2341 ccgtgtcggc cgggcacgcc atctacagcc ccgggccgat gctgtactgg ctgctcgcgc
     2401 tgcccgccta ctccggcgtc acggcgacgc tcactctcac gatcgcgacg atgaactgcg
     2461 ccgccatcgt cgggtcggtg gcgctcgcgc ggcgtcgcgg cggccttgcg ctgatgctcg
     2521 ccgccgccat tgcagtggcc ctgctgtccc ggtcgttctc gccggagagc ctccacgacc
     2581 tgttcaaccc gtcggccgcg ctctttccgc tcgtgctgct gatcttcctg tgctggtcgc
     2641 tcgcgtgcgg ggagtaccgc ctgcttccgc tcaccgcgct cgtcgcgtca ttcgtggcgc
     2701 aggctcacct cgcctacgtc gcgcctgtag ccggcgcgct ggccgtcggg cttgtcggcc
     2761 tggcgctgtc acggacccgg ctcgctcgcc tcgctactcc gccggcagcc ggcaggcgct
     2821 cgctcctggt ctggtcgctc gcggccgtcg cggtcacgct cgtctgctgg agcgctccga
     2881 tcgccgacca gatcgagcat caccccggga acctgagcct gatcgcgcag cgagccaccg
     2941 cgagcgggcc cacgcaggga gcgcagaagg gccgctgggc gttggaacgg gccgtgggcg
     3001 ttccgcctcg atggctgcgg tctccgacac gtggcagcgg gcagctcgct ctcgtgtccg
     3061 gaggggacta cggcgacacg cggctgagcg acgtgtcgca ggcgccgagc gccggtcaga
     3121 cgggctcggc gatagcgctg ctcgctgcgc tcctcgtcgt aatcgcgctc gccgcttggc
     3181 gcaggcgcgg cgacctggtg gccggagccg cgatcgccgt cgtgctgtgc gtggcgctag
     3241 tcgaggacgt ggccaagacg ccgatcacgg ccagcaacac cctcggctac tcgatctggt
     3301 ggggatcgat cgtcgggatg tgggcgtggc tgatcctggg ctggtcggcc tggtcgctgc
     3361 tcacagagac cgtcccgcgc ggttggcggc tccgggttcc ggcgatggcg cgcgccctcg
     3421 gcctcgccgc ggtggcggcg gtcgcgaccg cactcgccct agggcagggc gctgacaccc
     3481 atgcgccgta ctaccggccg ctgagcgctg tcgccggcgc gttggatcgc gccatcccgc
     3541 cgcaaacgac ggtcggcctg gtccagcgcg gcggcgttgc ggtgccgatc gagccggcga
     3601 tcaggtacac gttgcgcatc cacggcgtcc gcgctctcgg cgctttcgcg tcccggcggc
     3661 cgggcgcctg gtatgagctg gatcaccggc gggtcaactc ctgggtctcc gtcaacggcg
     3721 acaggccgtc tcggttcagg ccggcgcgag tggtcgcccg cgccgtcctc acggacccgc
     3781 agggaagcca ccgggtcact gcgacgctgt cgcctccgcg ggcggtgcgc gtctagcggg
     3841 cgcgcacctt gtccttgaag aaatcccaca ccgctgcggt ggccgagaag ccgggcgcac
     3901 cgaaggcgcc tggccattcg tggccgcgcc cgtaacgctt gatctgggca acctgcgtcc
     3961 ccgccttgca tttcgaccac gccacgagca cggtcgcgta ggccaggcgg cgcgcggcgg
     4021 tggacggcgc gcaggcgtcg cgctgcctcc atccctgcac gtaggcgggg acgctgcccc
     4081 tgtagtcagg cggtttgccg aagtacggga ccgcgttgtc gctcgtgccg tggatctcga
     4141 gcacggagag cgggcgatcg gcgtcgcagt cagggattgc tctgtagccg cccccaacgg
     4201 gcgcgattgc ggcgaagcgc tcgctgagcc cgcaaccgat cctggcggtg aagccgccgc
     4261 cgttcgacac gccggtcgcg tacacgcgat tgctgtcgac gcagaggcgc ttgcgaagcg
     4321 tgtcgatcag gtcgctgacg aagcgcacgt cctcccggcc gttcgggtag gtctcgttga
     4381 gactccagaa cgggtgcgac ccgcctgccg acggataggc gaccacgaac cgcttctggt
     4441 ccgccagccc cgacaggccg gagtagttct ccatgaactg cccggtcgct ccggctccat
     4501 ggaatgcgac gacgagcgga aggcgggtgc cgcggggagc gcggggcacg tgcacgcgcg
     4561 ccacccggtg ctgcccggcg ctcgtcatgt tgaggtcgta gctgccggtc cgatgcccag
     4621 ccccgcatcc ggacgctgga gccggcgttt gagccggtgc gggggccagc gctggaacgg
     4681 aggacgcaac taggagcacg accgcagcaa gcagtcgctt ggcactcact cggcggccat
     4741 cgtagcggcc gtttttaaag gcccagcgtc ttcccgagga tctccttcat gatctcgtcg
     4801 gtgcccccgc cgatcggtcc gaggcgcgtg tcgcgggcgg cgcgctcgat ctcgtattcc
     4861 ttcatgtagc cggcgccgcc gtggatctgg agcgcctcgt cggccacgtc gaaggcgtcg
     4921 cgctgggtct tgagcttcgc catcgtcacc tcctgcacgg cgtcgtggcc ctcggcgaac
     4981 agccgtaggg cgttgtaggt catcgcgcgg ccggtctcga gcttcaccgc catctccgcg
     5041 agcttgtgcc ggatcgcctg gtgcttggca atcgggcggc cgaacgcctg gcgttcgagc
     5101 gcgaactcga tcgtgcggtc gagcgtcacc tgcatcgatc ccacggcgcc gagcgccatc
     5161 agcagccgct cccactggaa gttggccatg atcaggtaga agccgcggtt ctcctggccg
     5221 agcaggttct cgtccggcac gaacacgtcc tggaaggcga gctcgccggt gtcggacgcg
     5281 tgccagccca tcttctcgag ctttcgcgac acgctgaagc cctccatgcc cttctcgatc
     5341 acgaggaacg agagcccgtg gtggccgccc tcccgcgtgg tcttcacggc ggtcacgacg
     5401 aagtccgcgc gtacgccgtt ggtgatgtac gtcttcgagc cgttcacgac gtagccgccg
     5461 tcgaccttct tggcgaacgt gcgtatgccg gccacgtcgg agccggcgtc cggctccgtg
     5521 atcccgagtg cggcgatctt atccccgcgg atggcaggcg cgagaaagcg ctgcttctgg
     5581 tcctcggtgc cgaacttcca gatcggcggt gtggcgatcc cgatgtgcgc cccgatgccc
     5641 gccgccagcc cgcccgagcc gcagcgtgat agctcctcgt tgaagacggc gtcgtgcagg
     5701 tagtcgccgc cctggccgcc gtactcctcc gggtacttca ggccgaggta gccgtgctcg
     5761 gcgagcttct cgaacacctc gttcgggaac cagcgcgcgt cctcccactc gcccgcatgc
     5821 gggcgaagct ccgtctcgac gaacgcgcgc gtgacctcgc ggaagcgctc gtgctcctcg
     5881 gtgaacggcg gcgccggctg gcggacggcg ggtgtgggga ggacgctcac agcgtggtgt
     5941 agccgcggcc cgcgtggtcg aagccgagca ggtcgccgga acggttcttc tcgttcgagt
     6001 ggtggtaggc gcgcacctcg gagatgcgcc cgtcccggaa gcggaaccac tctgtgccgc
     6061 ggtcgaggcg gcgatcgccg ctcttcggat cgcgccaggt catcgtccat tcgatcacgg
     6121 cctgctcacc atcttcgatg ccgttctcga tcgtccagcg gccttcgagg tgctcgaccg
     6181 ccagctgggt gtgctcgccg atcgtgcgcg cgcccgcgtg cgggccgagc cgcgtgtagt
     6241 agtgcacggc gtcgtcggtg aagaacgacg cgacctcttg gg
//
LOCUS       DASVXS010000043         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1142968, whole genome shotgun sequence.
ACCESSION   DASVXS010000043 DASVXS010000000
VERSION     DASVXS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142968"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VF752_02300"
     CDS             <1..1509
                     /locus_tag="VF752_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8854405.1"
                     /translation="RHEQTTVYAADGFARATGRPGIALTTTGPGAANTLGALGEAWAS
                     GSPVVVIATDIPAALRRPGVYRGTLHEATDQEAMFRPVTKHTMRVGDPAALGEAVVHA
                     CEIAVAPPSRPVYLEVPTDVLSAAVDEPTSPLPTPLSYPRHSPPENRLRDAAELIARA
                     ERPLIWAGGGALRADAREAVAELAERLCAPVITTYMARGLLGAQHPCAVGLPPHLPEV
                     GRIWDEADVVIAIGSDFDGMMTQNWAMPQPPALIAVNVDAADAAKNYHPDVLLEGDAF
                     TATAELARLVEPRGRIQELAHELAELRARVRDRLREEYPAEIEFLESFEGALPDDALV
                     VADMCIPGYWLGAFHPVPGPRRLAYPMGWGTLGYGFPLSIGSALAEEAPVVSVAGDGG
                     FLYAVGELATVAQEQIPLTQVIVDDGGYGMLRFDQQQAGDPTFGVDLDSPDFEALARS
                     FGVEAETVEGLGDEFAAALARQVNDERPTVLVARAALEPPPTTSPRWYRRRQ"
     gene            1509..2120
                     /locus_tag="VF752_02305"
     CDS             1509..2120
                     /locus_tag="VF752_02305"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX8854406.1"
                     /translation="MAFELDTPHGPARVHLQPVDDAVGALVLGHGAGGGVGAPDLVAA
                     AKAAEALGITVALVEQPYRVAGRRSQAPPAQLDAAWIAVVDQLSENGLSGLPLITGGR
                     SAGARVACRTAAKVGAVAVLCLAFPVHPPGKGDDPSKSRLPELDAVEVPVLVVQGDRD
                     PFGMPPDGPNRKVVRVPGNHSLRSTGAVGAAVSEWLAARMSMV"
     gene            2198..3748
                     /locus_tag="VF752_02310"
     CDS             2198..3748
                     /locus_tag="VF752_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8854407.1"
                     /translation="MERPVSPSDLLHAGLDENPDGLALISADTRWTWSMLDDFSDRLA
                     ASFLRLGLKPGDRVASLMPNRPALIVHYLACFKAGLVATPLNYRYMAPEIDHALAVSN
                     VRLLLAHAEREDDLAASELTPQLPLGKISYGASTGAGPVFDELVKGDRPSSPLPAPPP
                     GAPAVIFFTSGSTGRPKGVTHTHETLGWMLATGASGLELGPADVLLGGSSLSHVGAFY
                     VSFAALSVGARLIVARTFDADELLPLLREDRPTVLSMLPSALFALTRDHGARHDDFAS
                     LRLCRAAGDKVSAELEREFTALSGFPIDEAYGLTEMGLVTVSPPSARIKLGSVGQVVP
                     AVSLSIRNDEGDELPPGSEGRLWIKTPAATTGYWDDPDATDAAFSGAWFDSGDVMRAD
                     DEGYFCFCGRKKQIIVHDGSNICPQEVEGALLEHPSVASAGVIGIHDAVHGENVRAYV
                     TLTEGSKRPTRQELIKCARARVGYKAPEEIFFLDEMPRTVSGKVDRTSLKRMAEENLA
                     TVATANTN"
     gene            complement(3808..4122)
                     /locus_tag="VF752_02315"
     CDS             complement(3808..4122)
                     /locus_tag="VF752_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854408.1"
                     /translation="MSPPATLLMWFELRSPDLAEDFERLMTEDRDIVRGSLDTVSDWR
                     LTRPADVPGQSIGQADYVLIAEIVEVDRFEQQASEHVQRLVDDLAHLVSSRGMLVVRP
                     VL"
     gene            4190..>4371
                     /locus_tag="VF752_02320"
     CDS             4190..>4371
                     /locus_tag="VF752_02320"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX8854409.1"
                     /translation="MAAGAGQVIPELPIMRSRAEGEQRATFFELFFDLVYVFGVTQPS
                     HHLAGDISWEGAAQTA"
BASE COUNT          664 a         1498 c         1521 g          688 t
ORIGIN      
        1 cggcacgagc agacgactgt gtacgcggcg gacgggttcg cgcgcgccac cgggaggccg
       61 ggcatcgcgc tcaccaccac gggccccggc gcggccaaca cgctcggcgc tctcggggag
      121 gcgtgggcat ccggctcccc cgtcgtcgtc atcgccacgg atatcccggc cgcgctgcgg
      181 cgcccaggcg tgtaccgggg cacgctgcac gaggccaccg accaggaggc gatgttcagg
      241 ccggtgacca agcacacgat gcgagtgggc gacccggcgg cgctgggcga agcggtcgtc
      301 catgcgtgcg agatcgcggt ggcgccaccg agccggccgg tctacctgga ggtaccgacc
      361 gacgtgctct cggccgcggt ggatgagccc acctcgccgc tgccgacacc tctgtcctac
      421 ccccgtcaca gcccgccgga gaaccggcta cgcgacgcag cggagctgat cgcacgcgcg
      481 gagcggccac tcatctgggc gggcggcgga gcgctgcgcg ccgacgcgcg cgaggcggtc
      541 gcggagctgg cggagcgcct ctgtgcgccg gtgatcacga cctacatggc gcgcggcctg
      601 ctaggcgcac agcacccgtg cgcggtcggt ctccctccac acctgcccga ggtcggccgc
      661 atctgggacg aggccgacgt ggtgatcgcc atcggcagcg acttcgacgg gatgatgact
      721 cagaactggg ccatgcccca gccgcccgcg cttatcgccg tgaatgtgga tgccgccgac
      781 gccgcgaaga actaccaccc ggatgtcctc ctcgagggcg atgccttcac cgcgacggcc
      841 gagctcgcgc ggctggtcga gccgcgcgga cgcatacagg agctggcgca cgagctggcc
      901 gagttgcgtg cacgggtgcg cgaccggctg cgcgaggagt acccggcgga gatcgagttc
      961 ctcgagtcgt tcgagggcgc gctgcccgac gacgcgctcg tggtggcgga tatgtgcatc
     1021 cccggctact ggctcggcgc cttccacccc gtccctgggc cgcgccgact cgcctacccg
     1081 atgggctggg gcacgctcgg ctacggcttc ccgctctcga tcggctccgc gctcgcggag
     1141 gaggcaccgg tcgtgagcgt cgcgggggac ggcggcttcc tgtacgccgt cggcgagctc
     1201 gcgacggttg cgcaggagca gatcccgctc acccaggtga tcgttgacga cggcggctac
     1261 gggatgctgc gcttcgacca gcagcaggcg ggcgatccga ccttcggcgt ggacctcgac
     1321 tcacccgact tcgaggcgct cgcccgctcc tttggtgtcg aggccgagac ggtcgagggg
     1381 ctgggcgacg agttcgccgc cgcgctcgcg cggcaggtga acgacgagcg gcccacggtg
     1441 ctcgtggcac gcgccgcgct cgagccgccc ccgacgacct cgccgcgctg gtatcgcagg
     1501 aggcagtgat ggcgttcgag ctcgacaccc cgcacggccc ggcccgcgtc cacctgcagc
     1561 cagtcgacga cgcagtcggc gctctggtgc tgggccacgg cgccggcggc ggcgtcggcg
     1621 ctcccgatct cgtcgcggcc gcgaaggccg ctgaggcgct cgggatcacg gtcgcccttg
     1681 tggagcagcc ctaccgggtc gcaggccgcc gcagccaggc accgcccgcg cagctcgatg
     1741 cggcgtggat cgcggtggtc gatcagctga gcgaaaacgg gctgagcggc cttccgctga
     1801 tcaccggtgg ccgctcagcc ggcgcgcgcg tggcgtgccg cacggccgcc aaggtgggag
     1861 ccgtcgccgt tctctgcctc gcgttcccgg tgcacccgcc cggcaagggc gacgacccgt
     1921 cgaagagccg gctgccggaa ctcgatgccg tggaggtgcc ggtgctcgtg gtgcagggcg
     1981 atcgcgaccc cttcggcatg ccgcccgatg gccccaaccg gaaggtcgta cgcgtgccgg
     2041 gcaaccacag cctgcggagc accggcgcgg tcggagcggc cgtgtcggag tggctcgcag
     2101 cccgcatgtc catggtctga cggtccgcca aggattgcgt ttggtgtcgg gctccgtcag
     2161 tgtgggccca gcggtgaaca gttatgtcgg aaggacgttg gagaggccgg tcagccccag
     2221 cgatctgctt catgccggcc tggatgagaa ccctgacggg ctcgcgctca tctcagctga
     2281 cacgcgctgg acgtggtcga tgctcgacga cttcagcgat cgcctggccg cgagtttctt
     2341 gcgtctaggg ctgaagccgg gtgatcgcgt cgcttcgctg atgccgaacc gtcccgcgct
     2401 catcgttcac tacctcgcct gtttcaaagc ggggctggtc gcaacgcccc tcaactatcg
     2461 ctacatggca cccgagatcg accacgcgct cgcggtcagc aacgttcgtc tcctcctggc
     2521 gcatgccgag cgggaggacg atctcgctgc gagcgagctc accccgcagc tgccattggg
     2581 caagatcagc tacggcgcca gtaccggagc gggtccggtg ttcgacgagc tcgtgaaggg
     2641 agaccggccg tcttccccgc tgccggcccc tcccccaggc gccccggcgg tgatcttctt
     2701 cacctcgggc agcacgggac gcccgaaggg agtcacgcac actcacgaga cgctcggttg
     2761 gatgctcgcg acaggagcgt cggggctcga gcttgggcct gctgatgtcc tcctcggcgg
     2821 atcttctctc tcgcatgtcg gcgcgtttta cgtctcgttc gccgcgctga gcgtgggcgc
     2881 gaggttgatt gtggcccgga ccttcgatgc ggacgagttg ctcccgttgc ttcgtgagga
     2941 ccgcccgacc gtgctgtcga tgctgccgtc ggctttgttc gcgctcaccc gcgaccacgg
     3001 cgctcgtcac gacgatttcg cttcgcttcg actctgccgc gcggcgggcg acaaggtctc
     3061 cgccgagctc gagcgcgagt tcaccgcgct gagcgggttc ccgatcgacg aggcctacgg
     3121 gttgacggaa atggggcttg tgaccgtgag cccaccgtcg gctcgcatca agcttggttc
     3181 ggtgggtcag gtcgttccag ccgtctcgtt gtcaattcgg aacgatgagg gcgacgagct
     3241 gccgccggga agcgaaggcc ggctctggat aaaaacgcct gccgccacca ccggctactg
     3301 ggatgacccg gacgcgaccg acgctgcctt cagcggcgcc tggttcgact caggcgacgt
     3361 gatgcgagcc gatgatgagg ggtacttctg cttctgcggc cgcaagaagc agatcatcgt
     3421 ccacgacggc tcgaacatct gccctcagga ggtcgagggc gccctgctcg agcacccgag
     3481 cgtggcgagc gcgggggtga tagggatcca cgacgctgtc cacggtgaga acgtccgcgc
     3541 gtacgtcacg cttaccgagg gcagcaagcg tcctacccgc caggagctga tcaagtgcgc
     3601 gcgcgcgagg gtcggctaca aggcacccga ggagatcttt ttcctcgacg agatgccgcg
     3661 cacagtcagc gggaaggttg atcggacgag tctgaagcga atggccgagg agaatctcgc
     3721 caccgtggcc acggccaata cgaactagcc gcggccctta tcgcctttga gtgggataaa
     3781 ggcgatcgcc ctccaccgac cgcggattca aaggacggga cgcacgacga gcatcccccg
     3841 gctcgagaca agatgcgcca agtcgtccac caagcgttgg acgtgctcgc ttgcctgttg
     3901 ctcgaagcga tccacttcga cgatctcggc gatcagcacg taatcggctt gaccgatgga
     3961 ctgtccgggc acgtccgccg gccgcgtcag ccgccaatcc gaaaccgtgt ccagcgaccc
     4021 gcggacgatg tcgcgatcct ccgtcatcag ccgctcgaag tcctcggcga ggtccgggga
     4081 acggagctca aaccacatga gaagggtcgc cggagggctc atccgcgaag cgtaatggac
     4141 aagcgcagca atcccgttgc gacacgcaac gctcgcttat gctcgcgccg tggcggcggg
     4201 cgcaggtcag gtcattccgg agctgccaat catgcgcagc cgggcggagg gcgagcagcg
     4261 ggcgacgttc ttcgaactgt tcttcgatct cgtctacgtc ttcggggtca cccagccgtc
     4321 gcaccacctg gcgggagaca tcagttggga aggtgcggcg cagacggcgt t
//
LOCUS       DASVXS010000044         7585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_996923, whole genome shotgun sequence.
ACCESSION   DASVXS010000044 DASVXS010000000
VERSION     DASVXS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996923"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VF752_02325"
     CDS             <1..759
                     /locus_tag="VF752_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854410.1"
                     /translation="HSGPAVLRNVVARNRKVPLTDIEKVLGFTPGPSTGPAAAANLKP
                     YRIPLTRARIGFATSLPAFMYGSPSAGTDPCSDTATYYMRCLDKLGANVVMQDEANPG
                     SWAANGGGAYWQPLEWMRSTWRAASDKSVRFDYNVTPFLVGNLADLSFDGQTSITQGG
                     LKGRKCTYVGNSKLNRADGDPPSAAPNAGRKSEFLAIAPWVRRDGPRSALRRTGSRLA
                     GGSGDRLENAYVETAIAADLTFPPDPSRRGCLTG"
     gene            complement(760..1227)
                     /locus_tag="VF752_02330"
     CDS             complement(760..1227)
                     /locus_tag="VF752_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854411.1"
                     /translation="MAALPIPEGYHSVTPYLAVDNAAQAIDFYTRAFGAQERMRMDAP
                     EGKIGHAELQLGDSILMLSDPFPQSNTKAPKELGGTTVTLFLYVDDVDAVVQQAVDAG
                     ATLTGAVENMFWGDRSGSVTDPFGHQWQLATHVEDVPPDEMARRAREAMAGMA"
     gene            complement(1281..1970)
                     /locus_tag="VF752_02335"
     CDS             complement(1281..1970)
                     /locus_tag="VF752_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8854412.1"
                     /translation="MKLSILMPVFNEEPTVDEAVRQVLATELPVETELVLVDDGSTDR
                     TGEILRAADWPDDKVRIYHHERNQGKGAAVRTALEHAEGDIAAILDADLEYAPTDLAP
                     LLEPLLEGETNVAFGVRAFNGYTSHSFLYVLGNRGVTLLANVLFNVYIGDLMTCHKVI
                     RTDVFRDLPLCEKGFGIEPEITARLLQRGERIYEVPVSYRARSTEQGKKLTWVDGLRV
                     VRTLTRIRFGG"
     gene            complement(2000..2971)
                     /locus_tag="VF752_02340"
     CDS             complement(2000..2971)
                     /locus_tag="VF752_02340"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8854413.1"
                     /translation="MPRVLVTGGAGTIGTAVVRRLVADPDFEVRVSDQRPPAEWVREA
                     CEIHTGDLRDVAQAREAVAGVDRVIHLAAIVGGIGNFHKLPYTLLEVNTALYNGVFQA
                     SLEQRVDRLVYVSSSMVFERAREFPTTEEYISETPIPESAYGFSKLAGEIYCRALHDE
                     HELPFTICRPFNAYGPGEMPDDEPGIAHLVPDLIRKVLSGQRPLQIFGSGEQTRTITH
                     IDDIAEGIATATLHPAAENEDFNISASDERTVAEIARIVWEECGMPAEEFELEHLPSF
                     QVDVQRRWPSVEKAKRLLGFEARIGTRDGIRQTVDWLRENDPVLAER"
     gene            complement(2971..4200)
                     /locus_tag="VF752_02345"
     CDS             complement(2971..4200)
                     /locus_tag="VF752_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8854414.1"
                     /translation="MRSVAVVGLGRIGLPLALSFADHGYDVIGIDKDAVRLEEIAAGG
                     MPFEETGTQELLERVNASGRMRTSQRVEDAAEADSIVLTLGTPAHTHIEIDISDIRSV
                     VDQLLPVLREGQQVILRSTVAPGTTDWVAGYIEQRRGFTVGEDFFVAHVPERIAVNHF
                     LDEISSLPCIVGGVGEVSGLRAGELFEPFGAEIVQTTPVQAELAKIWSNIFRYVNFAV
                     PNLLMMNCEQFGANVFEVIDLVNHDYPRGGIAQPGLTAGTCLRKDFAFSEERSSAPGM
                     LLAVSRVHETMPLFLAEGLKKRLGGSLRDKKVAVLGLTFKRDSDDLRDSLSLKLIRLL
                     ERELAHVSRHDPYAPELSEPLESALAGADGVIVATNHSVYEDLAGKLPREAVLVDPWN
                     VSGAGRVFARAEELVTA"
     gene            complement(4210..4953)
                     /locus_tag="VF752_02350"
     CDS             complement(4210..4953)
                     /locus_tag="VF752_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX8854415.1"
                     /translation="MPIAITDYEGTMDVMDGMIARRERGYFCAVAVHATTVSYLDPEM
                     MEATASASLTVPDGMPVVWAANILGARLKSRVYGPELMDRYCARSAKKRHRVWLYGGR
                     DQGSLLQLATNLRRRHPGIEIVGGYSPPFRPLTEAEEADLVAQINRDRPDVLWVGIGV
                     PKQEKWLAQMRPKLDVPVMCAVGAAFDFLGGRVSQAPAWMQARGLEWIYRIAQEPRRL
                     LPRYLYYNPRFVLAFARQLVRQRLRGSRA"
     gene            5238..5954
                     /locus_tag="VF752_02355"
     CDS             5238..5954
                     /locus_tag="VF752_02355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854416.1"
                     /translation="MSATGTLTERLRARPPTVHAGKEYLGLAWPAIEWLEHNVREDMT
                     TLETGSGASTVVFAAAGATHTAVSPDPGEHEAIRAWCAAEGISREKVTFIPASSHTAL
                     QEKWQPQPLDLVLIDGAHSFPFPVLDWFFTAPHLKPGARVLVDDAYQPAVNVLARYLR
                     RSDHWELEEVLGHRTPAFRLVGDEALAFDWDDHALGHASFDYLPPGRRAVARLRYSAF
                     ERGPLKPLVPALGKLLGRGG"
     gene            5951..6934
                     /locus_tag="VF752_02360"
     CDS             5951..6934
                     /locus_tag="VF752_02360"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX8854417.1"
                     /translation="MSPRRSLITGIGGQDGSYLAELLLDRGHEVFGVLRRPAGEYPNL
                     ESLRGRIGLIEADLLDQASMERALRESGAEEVYNLASVSFVPRSWDEPVLTAEFAAVG
                     VTTMLEAVREVDPGISFYQASSSEIFGEPDSSPQNEDTPLRPLTPYGVAKAYGFFITR
                     SYRRRYGLHASSGILYNHESPRRPLEFLPRKVSNAVARIRAGLEHELVLGDLDAGRDW
                     GYAADYVRAMAMIVAAPEPADYVVATGVTHTVRELVQCAFAHAGLDWQEHVRVDPALQ
                     RGKAELHNLVGDASRARERLGWEPSVDFEGLVRLLVDADVAALDAQLAPEG"
     gene            6936..>7585
                     /locus_tag="VF752_02365"
     CDS             6936..>7585
                     /locus_tag="VF752_02365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854418.1"
                     /translation="MHVAIPMLTLVPGKMGGSETLTRGLLRGLAAAPGEGDRYTVVAE
                     PEGAASLRPLVREPVELRTVGFPRRGDSAAARVASVVRARYAGGRLRRAVSEAAGGPI
                     DVVHYALTVSVPELKLPRVVTVFDLQHELMPEFFSVAERAYRRVFYAGAIARADAIVT
                     ISEFSKQTIVERHRVDPERVVVAYQDVDRELFRPGPVSGDEALLEPLRLPERFVFYP"
BASE COUNT         1113 a         2637 c         2673 g         1162 t
ORIGIN      
        1 cacagtggcc ccgccgtgct ccggaacgtg gtggcgcgca atcgcaaggt cccgctcacc
       61 gacatcgaga aggtgctggg cttcacgccc ggcccgagca ccgggccggc ggcggccgcg
      121 aacctgaagc cgtatcggat cccgctaacc agggcgcgca tcgggtttgc gacgagcctg
      181 cccgccttca tgtacggcag cccgtcagcg ggcaccgacc cctgctcgga cacagcgacc
      241 tactacatgc gctgcctcga caagctcggc gccaacgtgg tcatgcagga cgaggcgaac
      301 ccgggcagct gggcggcgaa tggcggaggt gcctactggc agccgctcga gtggatgcgc
      361 tcgacctggc gcgccgcctc cgacaagagc gtgcgattcg actacaacgt cacgcccttc
      421 ctcgtgggaa acctcgccga cctctcgttc gacgggcaga cctcgatcac tcagggcggg
      481 ctaaagggcc ggaagtgcac gtacgtcggc aactccaagc tgaaccgggc ggacggcgac
      541 ccgccttcgg cagcgcccaa tgccgggagg aagagcgagt tcctggcgat cgcgccgtgg
      601 gtgcgcaggg acgggccgcg aagcgcgctg cgcaggaccg gctcgaggct cgccggcggc
      661 tcgggcgacc ggctcgagaa cgcctacgtg gagaccgcaa tcgccgccga cctcacgttc
      721 ccgccggacc cgagccgccg gggctgcctc actggctgat caggccattc cggccattgc
      781 ctcgcgggcg cgcctggcca tctcgtcggg cggcacgtcc tcgacgtgtg tggcgagctg
      841 ccactggtga ccgaacggat ccgtgacgct gccggagcgg tcgccccaga acatgttttc
      901 cacagcgccc gtcagggttg cgccggcatc gactgcctgt tgcaccaccg cgtcgacgtc
      961 gtccacgtag aggaagagcg tcacggtggt tccgcccagc tccttgggcg ccttggtatt
     1021 cgactgcggg aagggatcgg agagcatgag gatcgaatcg ccgagctgga gctcagcgtg
     1081 cccgatcttg ccctccggag cgtccatgcg catacgctcc tgcgcgccga aagcgcgcgt
     1141 gtagaagtcg atcgcctgcg ccgcgttgtc gacggccagg tacggggtca cggagtggta
     1201 gccctccggg atcggcagtg cagccatggt gtccctctcc tggggtcggg tttggacaac
     1261 agagaaatcg tacgcttgcg ctatccgccg aagcggatgc gagtgagcgt gcgcacaact
     1321 cgcaggccgt ccacccaggt gagcttcttg ccctgctcgg tggaacgggc gcggtaggag
     1381 accggcacct cgtagatgcg ctcgccgcgc tgcagcagcc gcgcggtgat ctcgggctcg
     1441 atgccgaagc ccttctcgca cagcggcaga tcacggaaca cgtccgtgcg gatgaccttg
     1501 tggcaggtca tgagatcgcc gatgtagacg ttgaagagca cgttcgcgag cagcgtcacc
     1561 ccgcggttgc cgaggacgta caggaacgag tggctcgtgt agccgttgaa cgcgcgcacg
     1621 ccgaacgcca cgttggtctc gccctccagc agcggctcga gcagcggggc gaggtcggtt
     1681 ggcgcgtact cgaggtcggc gtcgaggatc gcggcgatgt cgccctcggc gtgttcgagc
     1741 gccgtgcgca ccgcggcgcc cttgccctgg ttgcgctcgt ggtggtagat ccgcaccttg
     1801 tcgtccggcc aatcggcggc ccgcaggatc tcgccggtgc ggtcggtgga gccgtcgtct
     1861 acgagcacga gctcggtctc gacgggcagc tcggtggcga gcacctgccg caccgcctcg
     1921 tcgactgttg gctcctcgtt gaacaccggc atgaggatcg acagcttcat cgaggcgcgc
     1981 agagtagcgg ctgcggggat cagcgctcgg cgagcacggg gtcgttctcg cggagccagt
     2041 ccacggtctg cctgatgccg tcgcgggtgc cgatccgcgc ctcgaagccg agcaggcgct
     2101 tcgccttctc caccgacggc cagcggcgct gtacgtccac ctggaagctg gggaggtgct
     2161 cgagctcgaa ctcctcggcc ggcatgccgc actcctccca gacgatgcgt gcgatctcgg
     2221 ccacggtgcg ctcgtcggag gccgagatgt tgaagtcctc gttctccgcc gcggggtgga
     2281 gcgtggcggt ggcgatcccc tcggcgatgt cgtcgatgtg cgtgatcgtg cgcgtctgct
     2341 cgccggagcc gaagatctgg agcggccgct ggcccgacag caccttgcgg atgaggtcgg
     2401 gaaccaaatg cgcgatgccc ggttcgtcgt cgggcatctc gccggggccg tacgcgttga
     2461 acggcctgca gatcgtgaac ggcagctcgt gctcgtcgtg cagcgcgcgg caatagatct
     2521 cgcccgccag cttcgagaag ccgtaggccg actctggaat cggcgtctcc gagatgtact
     2581 cctcggtggt cggaaactcg cgcgcccgct cgaagaccat cgacgacgac acgtagacga
     2641 gccggtcgac ccgctgctcg agcgacgcct ggaagacgcc gttgtagagc gcggtgttca
     2701 cctcgagcag cgtgtacggc agcttgtgga agttgccgat gccgccgacg atcgcggcga
     2761 ggtggatcac gcggtccacg ccggccaccg cctcgcgcgc ctgggccaca tcgcgcaggt
     2821 cgcccgtgtg gatctcgcag gcctcgcgga cccactcggc gggcggccgc tgatcggaga
     2881 cgcgcacctc gaagtcggga tcggccacga ggcgccgcac cacagcggtg ccgatcgtcc
     2941 cggccccgcc cgtcacgagc actcgcggca tcaggcggtg accagctctt cggccctggc
     3001 gaacacgcgc ccggccccgc tcacgttcca ggggtccacc agcacggcct cgcgcggcaa
     3061 cttgcccgcg aggtcctcgt acacggagtg gttggtggcg acgatcacgc cgtccgcgcc
     3121 cgcgagcgcc gactcgagcg gctcggagag ctccggggcg taggggtcat gccgtgacac
     3181 gtgcgccagc tcgcgctcca gcagcctgat caacttgagc gagagcgagt cgcgcaggtc
     3241 gtcggagtcg cgcttgaagg tgagcccgag caccgccacc ttcttgtcgc gcagcgagcc
     3301 gccgagccgc ttcttcagcc cctcggcgag gaacagcggc atcgtctcgt gcacgcgcga
     3361 cacggcgagc agcatgcccg gcgcgctcga gcgctcctcc gagaacgcga agtccttgcg
     3421 taggcaggtg ccggccgtca gcccgggctg ggcgatgccg ccgcgcgggt agtcgtggtt
     3481 cacgaggtcg atcacctcga acacgttcgc gccgaactgc tcgcagttca tcatcagcag
     3541 gttcggcacc gcgaagttca cgtagcggaa gatgttcgac cagatcttcg cgagctcggc
     3601 ctgcaccggc gtggtctgca cgatctccgc gccgaacggc tcgaacagct caccggcgcg
     3661 caggcccgac acctcgccga cgccgcccac gatgcagggc agcgacgaga tctcgtcgag
     3721 gaagtggttc accgcgatcc gctcgggcac gtgcgcgacg aagaagtcct cgccgaccgt
     3781 gaagccgcgg cgctgctcga tgtagccggc cacccagtct gtggtgccgg gcgcgaccgt
     3841 cgagcgcagg atcacctgct ggccctcgcg cagcacgggc agcagctggt ccaccacgct
     3901 gcggatgtcc gagatgtcga tctcgatgtg cgtgtgcgcc ggcgtgccga gcgtcagcac
     3961 gatgctgtcc gcctcggcgg cgtcctccac gcgctgcgag gtgcgcatcc ggcccgaggc
     4021 gttcacgcgc tcgagcagct cctgcgtgcc cgtctcctcg aacggcatgc cgccggcggc
     4081 gatctcctcg agacgcacgg cgtccttgtc gatgccgatc acgtcgtacc cgtggtcggc
     4141 gaacgacagc gccagtggga gaccgatgcg gcccagccct acgaccgcga cacttctcat
     4201 ggacgaagct taggcccgcg acccgcgtag ccgctgacgc accagctggc gagcgaaggc
     4261 gagtacgaag cgcgggttgt aatagaggta gcgcggaagc agccggcgcg gctcctgggc
     4321 gatgcggtag atccactcga ggccgcgcgc ctgcatccac gcgggcgcct gtgagacgcg
     4381 cccgcccagg aagtcgaagg ccgcaccgac cgcgcacatc accggcacgt cgagcttcgg
     4441 gcgcatttgc gcgagccact tctcctgctt cggcacgccg atgcccaccc acagcacgtc
     4501 gggccggtcg cggttgatct gcgcgacgag atcggcctcc tcggcctcgg tgagcggcct
     4561 gaacgggggc gagtagccgc ccacgatctc gatccccggg tgccggcggc ggaggttggt
     4621 ggcgagctgc agcagcgagc cctggtcgcg gccgccgtag agccagaccc ggtggcgctt
     4681 cttcgccgag cgcgcgcagt agcggtccat cagctcgggc ccgtagacgc ggctcttcag
     4741 ccgggcgccc aggatgttcg ccgcccagac gaccggcatc ccgtccggca cggtgagcga
     4801 ggcgctggcg gtcgcctcca tcatctcggg atcgaggtag ctgaccgtcg tggcgtggac
     4861 cgcgactgcg cagaagtagc cgcgctcgcg gcgggcgatc atgccgtcca tcacgtccat
     4921 cgtcccctcg tagtccgtga tggcgatcgg cacgccgatc acgtcgcact tcgggatgtc
     4981 atctggccgc gggagcgtgg catcgcggga taccggagcg agcgcgctgg gcgcctgacg
     5041 gcgtgccggg acggctgttg cgatttctgg ttgaggactc actcgtctgc tggttcggcg
     5101 aggcatgccg cgcacctgcg gagcccctgc ccgtagcggg cgaagtgtag gggtctgcct
     5161 ggctggagtg cgctcagaag ctgagaacca ttgcgcgcag cggtagttgc ggcgagcgat
     5221 cgcgtatcgt cgccgcgatg tccgcgacgg ggacactcac cgagcggctg cgagcccgtc
     5281 cgcccacggt gcacgccggc aaggagtacc tgggcctggc ctggccggcg atcgagtggc
     5341 tggagcacaa cgtgcgcgag gacatgacca cgctcgagac cggctcgggc gccagcaccg
     5401 tcgtgttcgc ggccgcgggc gccacacaca ccgcggtgag cccggacccc ggcgagcacg
     5461 aggcgattcg cgcctggtgc gccgccgagg gcatctctag agagaaggtc acgttcatcc
     5521 cggcctcctc ccacacagct cttcaggaga aatggcagcc ccagccgctc gatctcgtgc
     5581 tgatcgacgg cgcccacagc ttccccttcc cggtgctcga ctggttcttc accgcgccgc
     5641 acctgaagcc tggcgcgcgc gtcctcgtgg acgatgccta ccagcccgca gtgaatgtgc
     5701 tggcgcgtta cctgcgccgg tccgatcact gggagctcga ggaggtgctc ggtcaccgca
     5761 ctcccgcgtt caggctggtg ggcgacgagg cgctcgcgtt cgattgggat gaccacgcgc
     5821 tcgggcacgc gagcttcgac tacctgcccc cgggccggcg cgccgtcgcc cggcttcgct
     5881 acagcgcctt cgagcgcgga ccgctgaagc cgctcgtgcc ggcgctcggc aagctgctcg
     5941 ggagaggcgg gtgagtccgc gccgctcgct gatcaccggg atcggcggtc aggacggctc
     6001 ctacttggcc gagctcctgc tcgaccgggg acacgaggtg ttcggcgtgc tccgccgccc
     6061 ggccggggaa tacccgaacc tcgagtccct caggggccgg atcgggctga tcgaggcgga
     6121 cctgctcgac caggcctcga tggagcgcgc gctccgcgag agcggcgccg aggaggtcta
     6181 caacctcgcc tcggtgtcat tcgtgccgcg ttcctgggac gagcccgtac tcaccgccga
     6241 gttcgccgcg gtgggggtca cgacgatgct cgaggcggtc cgcgaggtcg acccggggat
     6301 cagcttctac caggcttcct cgagcgagat cttcggcgag cctgacagct cgcctcagaa
     6361 cgaggacacg ccgcttcgcc cgctcacgcc ctacggcgtg gccaaggcgt acggcttctt
     6421 catcacccgc agctaccggc gccgctacgg cctgcacgcg tcctcgggga tcctctacaa
     6481 ccacgagtcg ccgcggcgcc cgctcgagtt cctgccgcgc aaggtgtcaa acgcggtcgc
     6541 acgcatccgc gcgggcctcg agcacgagct ggtgctgggg gacctcgacg ccgggcgcga
     6601 ctggggctac gcagccgact acgtgcgcgc gatggcgatg atcgtggcgg cgcccgagcc
     6661 ggcggattac gtggtggcga cgggcgtcac gcacaccgtg cgcgagctcg tgcagtgcgc
     6721 gttcgcgcac gccgggctcg attggcagga gcacgtgcgc gtggatcccg cgctgcagcg
     6781 aggcaaggca gagctccaca acctcgtggg cgacgcctcg cgagcccggg agcgcctcgg
     6841 ctgggagccg agcgtggact tcgagggact ggtgcgcctg ctcgtggatg ccgacgttgc
     6901 ggcgctcgat gcgcagctcg ctcccgaggg ctgacatgca cgtggcgata ccgatgctca
     6961 ccctggtccc ggggaagatg ggcggctccg agacgctgac gcgcggactg ctgcgcggcc
     7021 tcgcggcggc gccgggcgag ggcgaccgct acacggtcgt ggccgagccc gagggggccg
     7081 cctcgctgcg gccgctcgtg cgcgagcccg tcgagctgcg cacggtcggc ttcccgcggc
     7141 gcggcgactc cgccgccgcg cgggtcgcct cggtggtccg cgcgcgctac gcgggagggc
     7201 ggctgaggcg cgctgtcagc gaggcggccg gcgggccgat cgacgtggtg cactacgcgc
     7261 tcactgtctc cgtgcccgag ctcaagctgc cgcgggtggt caccgtcttc gacctccagc
     7321 acgagctcat gccggagttc ttctcggtcg ccgagcgcgc gtaccggcgc gtgttctatg
     7381 ccggcgcgat cgcgcgcgcc gacgcgatcg tgacgatcag cgagttcagc aagcagacga
     7441 tcgtcgagcg ccaccgtgtc gaccccgagc gggtcgtggt ggcctaccag gacgtggatc
     7501 gggagctgtt ccggcccggc ccggtgtccg gcgacgaggc gctgctcgag ccgctgcgcc
     7561 tgcccgagcg cttcgtgttc taccc
//
LOCUS       DASVXS010000045         8978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_76205, whole genome shotgun sequence.
ACCESSION   DASVXS010000045 DASVXS010000000
VERSION     DASVXS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76205"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VF752_02370"
     CDS             <1..538
                     /locus_tag="VF752_02370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854419.1"
                     /translation="VTGVDANPEAHEHACLKYARPGVKFVRDLVETYSEPCDAVVFLQ
                     TIEHVQDPKAVLDRFKAMLRPGGTAYVSTPNLLTLAPPGAEKSDNPWHVKEYRVEEFR
                     ALCEGSFDQVELLGLFHARKLRVHELAIKLGWDRVHKALGISKPFYDRFVPAISASDF
                     ELRSGPLERALDFLAILR"
     gene            535..2010
                     /locus_tag="VF752_02375"
     CDS             535..2010
                     /locus_tag="VF752_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEX8854420.1"
                     /translation="MSGRPPGGGRGALALVLHAHMPYVEGFGSWPFGEEWLFEAVASV
                     YLPLLELLEDAPPLTLGLTPVLCDQLEALKGEAGDRFVTFLREIRAPIHEEDADGLER
                     GGEPGLAQEVRRAAGDYRLADEAFEARGRDLLGAFRELSERSAIELWTSNATHAVMPL
                     LATDQGRRLQTTVGPASHRRRFGGWSGGFWLPECAYVPGLEHELAEEGVHAFCLDQTE
                     PLGLGAPEQLEPVLTAAGPIAVPIDWQTVELVWGDSGYPGDARYRDYHGRTIHDLKPW
                     NNGGGGYDHEAALVLAREHAGDFVARVCGRLEAYQRERARPGLVCCALDAELLGHWWY
                     EGPQFLSAVLEKAQATGLELVSLPQGLERFEPIERDLAPASWGKPKDLSTWDSPRVAG
                     MAFAARHAELRTLVAAAGAQERGGSALERAARELLAVQASDWAFLTTCDMAADYPQRR
                     VREHLDHLDAALAALTHSRPVPEPEVRNLAPDLDLGALLAP"
     gene            2021..3202
                     /locus_tag="VF752_02380"
     CDS             2021..3202
                     /locus_tag="VF752_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8854421.1"
                     /translation="MRVLILSWEYPPLIEGGLARHVRKLSENLVSQDVDVHVLTRGME
                     ESPAEEDLGGVVVHRVREPERPRELGEFVTWIEHMNADMLAAGVELGDRFEFDVVHGH
                     DWLVAVAGDHLAKRFRCPFVVTIHATEYGRHQGWVDKHPQSHIHGVEHWMSNRAERVI
                     ACSAYMREHVSDIYGLEEERITVIPNGIDPADLVPVDDLETLRSRFAQPDEELVLLVG
                     RLVYEKGFQFALEALPRLIQRLPQVRFIVAGSGTHEGELRKQATELGLDSRGTFLGWI
                     GDDVLHSLYRIADLTVVPSIYEPFGLVALEAMASGCPCLVADTGGLREVVPNENVGLR
                     FHSRDPDSLGEMAERLLTDEGLRDRLVAEAQEHVLSFDWADVARQVAGLYRETVRERV
                     G"
     gene            complement(3183..3602)
                     /locus_tag="VF752_02385"
     CDS             complement(3183..3602)
                     /locus_tag="VF752_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854422.1"
                     /translation="MNPLEQMLEKPRLADTLVAVWVVFWVAMGIWTAITVHGLKQVAD
                     TSIRAGDAVGQAGQVLSLLGAVPLVGGQLSAQADQIRALGDSARESGEKGKSSIDRLT
                     FLLGFSIAIVPSVPLLVFYIPARLASRREDGASPRAP"
     gene            complement(3599..4195)
                     /locus_tag="VF752_02390"
     CDS             complement(3599..4195)
                     /locus_tag="VF752_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854423.1"
                     /translation="MSASSDLHPAQHRALRELIAMATQLSRHWRALAERLPDGDIVKA
                     FRSGADSADQLMTEITPAARAHGLSVGPAAESAGRVLAGLRGNVRDRALERNQAARLA
                     SLDAQHVTTLLRYLHELSDANGPPDLAALCGVWDKRFTRVESSARRAAAALGHDPESA
                     IEPLDQSALGRAGQRLGSTIGGIGEASDRVLGLRRRNG"
     gene            complement(4192..4881)
                     /locus_tag="VF752_02395"
     CDS             complement(4192..4881)
                     /locus_tag="VF752_02395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8854424.1"
                     /translation="MDPSATAEERALDPEPIVRGAPPPLHGGLPTLLRFMRSHGMLNL
                     KYARLVLRLLRRRYLTAYGRRLKLDGLAFIGPKVTLEIGKNARVELGRWSWLGTGTKI
                     RCHEGVVSIGAKTVLGQECTLSAYQHISIGRECVIADRVMLIDFDHGMVEVERPIRLQ
                     GIYKRDTRVGNNVWIGYGACILRGVTVGDNAVIGTNSVVTRDVPPNAVVGGVPAKVIR
                     MREEPRTLRWE"
     gene            complement(5198..5614)
                     /locus_tag="VF752_02400"
     CDS             complement(5198..5614)
                     /locus_tag="VF752_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854425.1"
                     /translation="MSTQTVGRVHVSEIEPHDSCQGDEPQWLPVRHQFDVKSFGVNAW
                     RAPSAGAPVIEDHTETEGGADGHEELYFVASGHARFTVGEEEIDAPAGTFVYVRDPSL
                     RRSGVGIEDGTTVLAMGGRPGKAFRVSPWEKRHFGD"
     gene            complement(5713..6651)
                     /locus_tag="VF752_02405"
     CDS             complement(5713..6651)
                     /locus_tag="VF752_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8854426.1"
                     /translation="MRVFVAGAAGVIGRSLVPMLVKAGHEVTGGTRSEARADDIRAMG
                     AEPVIRDVFDAEAVKRAVAAARPEVLINELTALPTSINPRARSEQFAGTNRLRREASR
                     ILMDAAAEAGARRVVAQSIAFVYRLTGEGLKSEDDDLFLEAPPPSGEVVAAVAELERN
                     VTRDDRVEGVVLRYGYFYGPGTAYARDGSFADLARRRRLAIVGPGTGVFSFIHVDDAA
                     RATVAALDRGSSGIYNVVDDDPAPVRDWVPAFAEAVGAPKPRRVPAWLARVVAGRYTV
                     ELMTASRGASNAKAHAELGLDLRFPSWREGFREGLG"
     gene            6810..7871
                     /locus_tag="VF752_02410"
     CDS             6810..7871
                     /locus_tag="VF752_02410"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8854427.1"
                     /translation="MASSTQESYAESIGGVPNAAPASDRAFWRAGLAPYARPHLGKSL
                     LDLVTSLLPYLALSVLMYVALDVSYLLVLALAIPAGGFLLRTYIVFHDCAHGSFLPSK
                     RANAWLGTVLGLVVYSPFLSWRHSHAVHHATAGDLDRRGVGDVPTMTVAEYRSSRWPR
                     RLGYRVFRNPAVMFGLGPIYALVLQPRLVSRSARPRIRRSVMATNVVLAVLVAALCWL
                     IGWRDYLLVQAPTALLAGSAGVWLFYVQHQFEDTYWQSAGDWSYADAALQGSSYLKLP
                     KLLQFFTGNIGLHHVHHLSARIPNYNLQRAHDTNPIFHEVPTLSLWDGLRAVRLKLWD
                     EDRRRLVTFADARADRPAA"
     gene            7874..8776
                     /locus_tag="VF752_02415"
     CDS             7874..8776
                     /locus_tag="VF752_02415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854428.1"
                     /translation="MATRDDLEFTYSLIDRIFRLSLGELADFSGAKYDGDFSLSLEEA
                     QRRKHEYVADQIGIGPGRRVLDLGCGWGPLLDFVRRRGADGVGVTLSSAQAAACRRHG
                     LDVRLRDAREVTREELGTFDAIASLGAFEHFCSPGDYRAGRQEEVYGDLFSRIASLLP
                     DSGRLYLQTMVFGRNMIPPDEVDIDAPRDSDAWYLALMERQFPGSFLPFGQEQIVRTA
                     TPHFRLVSSTSGRLDYIETIRQWRKRFAAPSARKRLLKLQLVPRWLTSRDFRLAFTSG
                     VSANGVCFERELLDHYRLVFEKAV"
BASE COUNT         1341 a         3144 c         3095 g         1398 t
ORIGIN      
        1 cgtgaccgga gtggacgcga accccgaggc gcacgagcac gcgtgcctga agtacgcgcg
       61 ccccggcgtg aagttcgtgc gcgacctcgt ggagacctac agcgagccgt gcgacgccgt
      121 cgtgttcctc cagacgatcg agcacgtgca ggatccgaag gccgtcctcg accgcttcaa
      181 ggcgatgctc cgtccgggcg gcaccgcgta cgtgtcgacg cccaacctgc ttacgctcgc
      241 cccaccgggc gccgagaagt cggacaaccc gtggcacgtg aaggagtacc gcgtcgaaga
      301 gttccgcgcg ctgtgcgagg ggagcttcga ccaggtagag ctgctcgggc tcttccacgc
      361 gcgcaagctg cgcgtgcacg agctggcgat caagctcggc tgggaccgcg tccacaaggc
      421 gctcgggatc agcaagccgt tctatgaccg cttcgtaccg gccatctcgg cgagcgactt
      481 cgagctccgc tccgggccgc tcgagcgcgc gctcgacttc ctggcgatcc tgcgatgagc
      541 gggcgcccgc ccggcggcgg gcgtggagcg ctcgcgctcg tgctccacgc ccacatgccg
      601 tacgtggagg gattcggcag ctggccgttc ggcgaggagt ggctgttcga ggcggtcgca
      661 agcgtgtacc tgcccctact cgagctgctc gaggacgcgc ctccgctgac actcggactc
      721 acacccgtgc tctgcgacca gctcgaggcg ctcaagggcg aggcgggcga ccgcttcgtg
      781 accttcctgc gcgagatccg cgcgccgatc cacgaggaag acgctgacgg actcgagcgc
      841 ggcggcgagc ccggcctcgc ccaggaggtg cgccgcgcgg cgggcgacta ccggctcgcc
      901 gacgaggcgt tcgaggcgcg cggccgcgat ctactgggcg cgtttcggga gctgtccgag
      961 cgctccgcga tcgagctctg gacgagcaac gccacgcacg ccgtcatgcc gctgctcgcc
     1021 accgaccagg gcaggcgcct gcagaccacc gttgggccgg cgtcgcaccg ccggcgcttc
     1081 ggcggctgga gcggcggctt ctggctgccg gaatgcgcgt atgtccccgg gctcgagcac
     1141 gagctcgcgg aggagggggt acacgccttc tgcctcgatc agacggagcc tctcgggctc
     1201 ggggcgcccg agcagctcga gccggtgctc accgcggcgg ggccgatcgc ggtgccgatc
     1261 gactggcaga cggtagagct cgtctggggt gacagcggct acccgggcga cgcccgctac
     1321 cgcgactacc acggccgcac gatccacgac ctgaagccgt ggaacaacgg cggcgggggc
     1381 tatgaccacg aggcggcact ggtgctggcg cgcgaacacg cgggcgactt cgttgcgcgg
     1441 gtctgcggcc gcctcgaggc ttaccagcga gagcgcgcca gaccgggcct cgtgtgttgc
     1501 gcgctcgacg ctgagctgct cggccactgg tggtacgagg gtccgcaatt cctctcggcc
     1561 gtactcgaga aggcgcaggc gacgggactc gagctcgtct cgcttccgca gggtctagag
     1621 cgtttcgagc cgatcgagcg cgaccttgca cccgcaagct gggggaagcc gaaggacctg
     1681 agcacctggg actcgccgcg cgtcgcggga atggctttcg cggcgcgtca cgccgagctg
     1741 cgcacgctcg tcgctgcggc cggcgcacag gagcgcggcg gctccgcact cgagcgcgcg
     1801 gcccgcgagc tgctcgccgt ccaggcgagc gactgggcct tcctcaccac ctgcgacatg
     1861 gccgcggact acccgcagcg gcgcgtccga gagcacctcg atcacctcga cgccgcgctt
     1921 gccgctctga cacactcccg ccccgtgcca gagccggaag tgcgcaatct ggctcccgat
     1981 ctcgacctcg gcgcgctgct cgcgccgtga ccgggaagcc atgcgagtcc tgatcctgtc
     2041 ctgggagtac ccgccgctca tcgagggcgg gctcgcgcgt cacgtgcgca agctctcgga
     2101 gaacctcgtg tcccaggacg tggatgtgca cgtactcacg cgcggcatgg aggagtcgcc
     2161 ggcggaggag gacctcggcg gcgtcgtggt gcaccgcgtc cgcgagcccg agcgcccgcg
     2221 ggagctcggc gagttcgtga cctggatcga gcatatgaac gccgacatgc tcgccgccgg
     2281 cgtcgagctc ggcgaccgct tcgagttcga cgtggtgcac ggccacgact ggctggtggc
     2341 ggtggcgggc gaccacctcg cgaagcgctt ccgctgcccc ttcgtcgtca cgatccacgc
     2401 gaccgagtac ggccgccacc agggctgggt cgacaagcac ccgcagtcgc acatccacgg
     2461 cgtcgagcac tggatgtcga atcgagcaga gcgcgtgatc gcctgttcgg cgtacatgcg
     2521 cgagcacgtg tccgacatct acgggctcga ggaggagcga atcaccgtga tcccgaatgg
     2581 gatcgacccc gcggacctcg tgccggtcga cgacctcgag acgctgcggt cgcgcttcgc
     2641 acagccggac gaggagctgg tgctgctcgt cggccggctc gtgtacgaga agggcttcca
     2701 gttcgcgctc gaggcgctgc cgcgcctgat ccagcggttg ccccaagtgc gcttcatcgt
     2761 cgccggctca ggcacgcacg agggcgagct ccgcaagcag gcgaccgagc tcggactcga
     2821 ctcgcgcggg acgttcctcg gctggatcgg cgacgacgtg ctgcactcgc tctaccggat
     2881 cgccgacctc acggtggtcc cctcgatcta cgagccgttc gggctggtgg cgctcgaggc
     2941 gatggcgtcg ggctgcccgt gcctcgtggc cgacaccggc gggctccgcg aggtggtgcc
     3001 gaacgagaac gtggggctcc gcttccactc gcgcgacccc gactcgctcg gcgagatggc
     3061 cgagcggctg ctcaccgacg aggggctgcg cgatcggctc gtggccgagg cccaggagca
     3121 cgtgctgagc ttcgactggg ccgacgtggc gcggcaggtg gcagggctct accgcgagac
     3181 cgtcagggag cgcgtgggct agctccgtcc tcgcgccgcg aagccagccg cgcggggatg
     3241 tagaacacga gcagagggac gctcggcacg atcgcgatcg agaagccgag caggaatgtg
     3301 agccggtcga tgctcgactt gcccttctcg ccgctctcgc gggcgctgtc gccgagcgcg
     3361 cggatctgat ccgcctgagc cgacagctgg ccgcccacga gcgggaccgc gcccagcagg
     3421 ctgagcacct gacctgcctg gcccacggcg tcgcccgccc ggatcgacgt gtcggcgacc
     3481 tgtttcagcc cgtggacggt gatcgccgtc cagatgccca tcgctaccca gaagacgacc
     3541 caaaccgcga cgagcgtgtc ggccagccgc ggcttctcga gcatctgctc gagcgggttc
     3601 atccgttccg ccggcggagg cctagtacgc ggtcgctcgc ctcgccgatc ccgccaatcg
     3661 tgctcccaag ccgctgcccg gcccgaccca gcgcggactg gtcgagcggc tcgatcgccg
     3721 attcagggtc gtgtccgagc gcggccgccg cgcgccgcgc gctcgactcc acgcgcgtga
     3781 agcgcttgtc ccacacacca cagagcgctg cgagatccgg cggcccgttc gcgtccgaca
     3841 gctcgtggag gtatcgaagc agcgtggtca cgtgctgcgc gtcgagcgag gcgagtcgtg
     3901 ccgcctggtt gcgctcgagc gcgcggtcgc ggacgttccc gcgcaggccg gcgagcacgc
     3961 ggccggcgct ctccgccgcc gggccgacgc tgagcccgtg cgccctcgcg gccggcgtga
     4021 tctccgtcat gagctggtca gcgctatcgg cgcccgacct gaacgccttc acgatgtcgc
     4081 cgtcgggcag ccgctccgcc agcgcacgcc agtgccggga gagctgagtg gccatcgcga
     4141 tgagctcgcg aagcgcgcgg tgctgcgcgg ggtggaggtc cgaggacgcg ctcactccca
     4201 gcgcagcgta cgcggttcct cgcgcatgcg gatcaccttg gccgggacgc cgccgacaac
     4261 ggcgttcggc ggcacgtcgc gcgtgacaac cgagttggtg ccgatcacgg cgttgtcgcc
     4321 gaccgtgacg ccgcgaagga tgcaggcgcc gtagccgatc cagacgttgt tgcccacgcg
     4381 tgtgtcgcgc ttgtagatcc cctgcagccg gatcgggcgc tccacctcga ccatcccgtg
     4441 gtcgaagtcg atcagcatca cgcggtcggc gatcacgcac tcgcgtccga tggagatgtg
     4501 ctggtaagcg gacagcgtgc actcctggcc gagaacggtc ttggcgccga tcgagacgac
     4561 tccctcgtgg cagcggatct tcgtgcccgt gccgagccag gaccagcggc cgagctccac
     4621 gcgggcgttc ttgccgatct cgagcgtgac cttggggccg atgaacgcga ggccgtcgag
     4681 cttgagccgg cgcccgtagg cggtgaggta gcggcggcgc agcaggcgca ggaccagccg
     4741 cgcgtacttg aggttgagca tcccgtgcga gcgcatgaag cgcaggagcg tcgggagtcc
     4801 gccgtggagc gggggcgggg caccgcggac gatcggctcg ggatcgagcg cgcgctcctc
     4861 ggctgtcgcg gacggatcca tcgcgagccg gatcatagtt tcggccctac agcgcgccgc
     4921 ttgcccagcg aggatccggc gcggagggcg actccgctct ttcgcgacct gctaggccag
     4981 gagcgggaga aacgtccatc cggtgccttt ctcccggtcg agatgtacga agctcccgca
     5041 tagcgggaga aacgtccctt ccattccgga tggcgcctcg agatgcacga aattttcgcc
     5101 tcgagggcac aactcctgac ttttatgccg ctatgcggca agaaactcag gaggtggcga
     5161 gcaagcccag ggtggctgga ccggagctgg ccctcgttca atcgccgaag tggcgcttct
     5221 cccacggcga cacgcgaaac gctttcccag gccgtccgcc catcgccagc acagtcgtgc
     5281 cgtcctcgat gccgacgccg gaccgccgca gcgacgggtc gcggacgtag acgaatgtgc
     5341 ccgccggagc gtcgatctcc tcttcgccca ccgtgaagcg tgcgtggccg gaggcgacga
     5401 aatagagctc ctcgtggccg tcggcgccgc cctcggtctc ggtgtggtct tcgatcacag
     5461 gggcgccggc ggacggggcg cgccacgcat tcacgccgaa cgacttgacg tcgaactgat
     5521 gccgcacggg cagccactgc ggctcgtcgc cctggcagct gtcgtgcggc tcgatctctg
     5581 agacgtggac cctgccgact gtctgggtgc tcatgttggc ctccttctcc gtcaccgtcg
     5641 ctatcgcttt accggttcca gatcaccagt aaaccagaga cgctggtgac agcgcaatga
     5701 cgcgagccac actcacccaa gcccctcgcg gaagccctcg cgccagctcg gaaagcgcag
     5761 gtcgagcccg agctccgcgt gcgccttggc gttcgaagct ccccgcgaag cggtcatcag
     5821 ctcgactgtg tagcggcccg ccaccacccg cgcgagccac gccggcacgc ggcgcggctt
     5881 cggcgcgccg acggcctcgg cgaacgcggg gacccaatcg cggaccggcg ccgggtcgtc
     5941 gtcgacgacg ttgtagatac cggatgaccc gcgatcgagc gccgccacgg tggcgcgcgc
     6001 agcgtcatcc acgtggatga aggagaagac gcccgtaccg ggaccgacga tcgccaggcg
     6061 gcgcctgcgc gcgaggtcgg cgaacgagcc gtcgcgggcg taagccgtgc cgggcccgta
     6121 gaagtagccg tagcggagca cgacgccctc cacccggtcg tcacgggtca cgttccgctc
     6181 gagctcggcg acggcggcga ccacctctcc cgacggcggc ggggcctcga ggaacaggtc
     6241 gtcgtcctcg ctcttcagcc cttcgccggt gaggcggtag acgaacgcga tgctctgcgc
     6301 gaccacccgg cgggcgcccg cttcggcggc ggcgtccatg aggatgcggc tggcctcgcg
     6361 gcgcagccgg ttcgtgccgg cgaactgctc gctacgggct ctcgggttga tgctggtggg
     6421 cagagcggtg agctcgttga tcagcacttc ggggcgcgcc gcggccaccg cgcgcttcac
     6481 ggcttcggcg tcgaacacgt cgcggatcac cggctcggca cccatcgcac ggatgtcatc
     6541 cgcgcgcgcc tcgctgcgcg tgccgccggt cacctcgtgg cccgccttca cgagcatcgg
     6601 cacgagcgac ctgccgatca cacctgcggc tcccgccacg aacacccgca tcggcgcagc
     6661 ctaatgcgtt gcacaacttc aaccggtacg ttttcgtgcg cccggtaccg gggtggatcg
     6721 ccgacgcgac gctgggtagg ctgatggtgg actgcggaac agggcgccgg tggatctgcg
     6781 gtcgccgccg ttgagcaaat ggaggggcga tggcaagcag cacccaagag tcctatgcgg
     6841 agtccatagg aggagtgccg aacgcggcgc ccgcaagcga tcgcgcgttc tggcgggcgg
     6901 gtctcgctcc gtacgcacgg ccgcaccttg gcaagagcct gctcgatctc gtcacgtcgc
     6961 tcttgccgta cctcgccctt tcggtgctca tgtacgtggc gctcgatgtg tcatacctgc
     7021 tcgtgctggc gctcgcgatc ccggccggcg gcttcctgct gcgcacctac atcgtgttcc
     7081 acgactgcgc ccacggctca ttcctaccgt caaagcgcgc gaacgcctgg cttgggacgg
     7141 tcctggggct cgtcgtgtat tcgccgttcc tgagctggcg ccacagtcac gcggtgcacc
     7201 atgcgacagc cggcgatctg gaccggcgcg gcgtgggaga tgtgcccacg atgaccgtcg
     7261 cggaatatcg ctcgtcgcgc tggccgcgcc gactcgggta ccgcgtgttc cggaaccccg
     7321 cggtgatgtt cggcctcggc ccgatctatg ccctcgtcct tcaaccgcgc ctggtgtccc
     7381 gatcggcgcg gccacgcatc cggcgcagcg tgatggccac gaacgtggtg ctcgcagtgc
     7441 tcgtggcggc cctgtgctgg ttgattggtt ggcgcgacta tctgctcgta caggcaccca
     7501 ccgcattgct cgccggctcg gccggcgtgt ggctcttcta cgtacagcac cagttcgaag
     7561 acacgtactg gcagagcgcc ggcgactgga gctacgcaga cgcggcgctg cagggcagca
     7621 gctacctgaa gctgccgaag ctcctccagt tcttcacggg gaacatcggc ctgcaccacg
     7681 tccaccatct gagcgctcgc atcccgaact acaacctcca gcgcgcgcac gacaccaatc
     7741 cgatcttcca cgaggtgccg acgctgtcgc tgtgggacgg cctccgcgcc gtgcggctca
     7801 agctgtggga cgaggaccgc cggcggctcg tcaccttcgc cgacgcgcgc gctgaccggc
     7861 cagccgccta accatggcca ctcgggacga cctcgagttc acctattcgc tgatcgatcg
     7921 catattcagg ctcagcctgg gagagctggc ggacttcagc ggagcgaagt acgacggcga
     7981 cttctcgctg tcgctcgagg aggcgcagcg tcgcaagcac gagtacgttg cggaccagat
     8041 cgggatcggc cccggccgga gggtgctcga cctgggctgc ggctggggtc cgttgctcga
     8101 cttcgtccgg aggcggggag ccgatggggt cggcgtcacc ctctcgagcg cacaggcggc
     8161 agcctgtcgc cgtcatggtc tcgacgtccg cctccgtgat gcgcgtgaag tcacacgtga
     8221 ggagctcggc acgttcgacg ccatcgcaag tctcggcgcc ttcgagcact tctgctcgcc
     8281 tggggactat cgcgccgggc ggcaggagga ggtctacggc gacctattct ccagaatcgc
     8341 cagcctgctg cccgacagtg gccggctcta cctccagacg atggtgttcg gccggaacat
     8401 gatcccgccg gacgaggtcg acatcgacgc accgcgggac tccgacgcct ggtatctcgc
     8461 gctgatggag cggcagtttc cgggctcctt cctgcccttc ggccaggagc agatcgtgcg
     8521 caccgcgacg ccgcactttc gcctggtctc gagcacaagt ggccggctcg actacatcga
     8581 gacgatcagg cagtggcgga agcgcttcgc cgctcccagc gcgagaaaga ggctgctcaa
     8641 gctccagttg gtcccacgct ggctcacgag ccgggacttt cgacttgcgt tcacctctgg
     8701 ggttagcgcg aacggtgtct gcttcgagcg cgagctgctc gatcactacc ggctcgtgtt
     8761 cgagaaagcc gtttgagcga tcgggcaagt tgttgaccag ggcgctgggg aggcgtcatt
     8821 gtcttcggat ggccaccact agagccgacc tcccgaacag cgcgaacgcc gaggcgatcg
     8881 aggcctggga cggaccgctc tacgaccgct tcgtccgctt ccgccatctc gtcaccaccg
     8941 gactcgcgcc ccacagcgag gaggcgctcc gcctgctg
//
LOCUS       DASVXS010000046        15275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_930996, whole genome shotgun sequence.
ACCESSION   DASVXS010000046 DASVXS010000000
VERSION     DASVXS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15275
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930996"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VF752_02420"
     CDS             complement(<1..1402)
                     /locus_tag="VF752_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8854429.1"
                     /translation="MALQACATCGFENPAEARFCMSCGTALERACPSCGTPAPPGARF
                     CMACGTALDGSVAQPSPVPAPPAPAPLPEERRQVTVVFADLSGYTAVAEQYDPETVKA
                     LVDQCLRRFGNEVERYGGTVDKYIGDNVMAVFGAPVSHEDDAERAIRAALAMQDAMRE
                     INSELELSHGVSFELRVGVNTGEVLAGAVGEAYTVIGDTVNVASRLQAAGRPGTVTVG
                     PRTRDVTSHAVEYRQLEPLELKGKAEPVQAFEVVGLLAAHPVRRELARSEAPLVGRDD
                     ELDLLKSLYGRVRRERRPHLITLVGQAGVGKSRLLREFEKSLGAEGPHVFRQGRCLPY
                     GSSIVYWPLGEVLRAECGIVDGDPSDIAWQKLSTRLLELWGEGEEAGERRAALIGRLL
                     GIEAPVELASGESQDPQRIRETFFAAIRSSIEGMARETPLVLAFEDIHWADQGMLDLI
                     EHLAQWARAPVMILCLA"
     gene            complement(1507..1842)
                     /locus_tag="VF752_02425"
     CDS             complement(1507..1842)
                     /locus_tag="VF752_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854430.1"
                     /translation="MPVVELAEHSRQLGRRPALHEADVHAAAHQALDRRHRVTPSFGR
                     GQHRPRLREQGLSRVAELDVSRAPFEEAGAELLLQGADRGRQPGLHDVNAARGARELP
                     LFGDRDEVL"
     gene            1956..2393
                     /locus_tag="VF752_02430"
     CDS             1956..2393
                     /locus_tag="VF752_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8854431.1"
                     /translation="MHVRPLGELRMVLAVSAQHRLANRKAVTLQALREETFVDVSRDW
                     GTRLITDRALAAAAIERRVAFELNDLFTAMDLISAGLAVAVMPELEMTRNDGLKLIPI
                     TGSRMVWEISMVSREGDLSPAAEELIRHLLEARGGPLPISTRD"
     gene            2483..3196
                     /locus_tag="VF752_02435"
     CDS             2483..3196
                     /locus_tag="VF752_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8854432.1"
                     /translation="MRARGEGEPAANRTSLLSRREREVLEQVALGNSTTAIAEELVLS
                     PHTVRAHLKNIHRKLETTSRAHAVALLMEERGEDVPTAAGQDARRIVVKALARALDAR
                     DPYTSSHSEDVTLLARLVAQELGMSGTDVRDVEDVAALHDIGKLGVPESILWKPGPLD
                     PVEETVMRRHAEIGAAIVAHVEPIAHLAIAVRAEHERWDGNGYPDGIAGGEIPIASRI
                     VLACDSYDAMVTDRAYRGR"
     gene            3464..4738
                     /locus_tag="VF752_02440"
     CDS             3464..4738
                     /locus_tag="VF752_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8854433.1"
                     /translation="MRHQLHRWAITAAVACVCALAAPGTAGAAEPTVTLGAAGNIACG
                     PLQAPSTSECQQDATASLMTSLDPDAVATLGDQQYDSATASEYSQFYANSWGTMTVPV
                     HPVPGNHEYMDPAGNAAGYFGYFGPAAGNPSRGYYSYDLGSWHIIALNSACNVTHNCA
                     PGSAQERWLRADLAAHPRVCTLAYWHHPLFTTGETRGDPNNAATRSFWHALYRHGADI
                     VLNAHDHSYQRFAPQTPNGASDPAQGIREFIVGTGGRSLFTGTVPAANLQVRDISNFG
                     VLLLTLDSGHYSWRFYPTQSGGFTDSGSGRCHETTPVLRLRAHWLRIAVKTGMGAARA
                     KCDSAAGDRCVVRVTLFVRGRKVGTGRATIPAGKLGMLRVRLNRNGRLMLSRSRTHKL
                     RVRAVGAPKNHAGVAGPIDRTLMLKGMARRRR"
     gene            complement(4920..4992)
                     /locus_tag="VF752_02445"
     tRNA            complement(4920..4992)
                     /locus_tag="VF752_02445"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4957..4959),aa:Ala,seq:tgc)
     gene            5114..6460
                     /locus_tag="VF752_02450"
     CDS             5114..6460
                     /locus_tag="VF752_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX8854434.1"
                     /translation="MTTESAGTTEELHGNGHGPPDKSPRQANLAVRAQRAEISNLEIV
                     NHWFSLAADRLGLREDIAAVLRSSYREVQVQIPVKLGDGKIHVFSGYRVQHNGARGPY
                     KGGVRFHPEVDLDEVRALAALMTWKTAVTGIPYGGAKGGVNVPASDLSQSELERISRS
                     FIDKVDKMLGPTRDIPAPDVNTNAQTMAWMMDEYGKLHGHTPACVTGKPISLEGSYGR
                     EAATGRGLIYLLREAAPRLDLTPSGTRIVVQGFGNVGSWAARIAQQLGCRIVGVSDAN
                     GAIASEAGIDVEALHQHIQLGGLITDFDGVEAITPDELLALECEVFIPAALGGMIHKE
                     NADLLNCRMVIEGANSPTTPAADEILSDKGIYVVPDVLANAGGVIVSYFEWVQNLQHF
                     RWDEREVNDRLGTIIRKAFREVAAKARKENDTLRVAAYELGIERVVEAATTRGYIS"
     gene            complement(6506..7183)
                     /locus_tag="VF752_02455"
     CDS             complement(6506..7183)
                     /locus_tag="VF752_02455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854435.1"
                     /translation="MPNPATGDNARERNRALFDGAFGAVYGLYIERERLSRLIAALVW
                     GSDSRPFYASMRAIGAVPDGGLIVDAPCGAGVAFRGLRPSQHVRYIALDLSSRMLERA
                     RHRAARLGLGQIEFVQGDAQAIPAEDSSVDLFLSYWGLHCFPDPSCAVAEMARCLRPG
                     ARVVGGMITRGQTARHRLLVHPGRGGFGPGGTVDDLQRWLDDAGFQETTTDTSGLFAY
                     FEAVKAT"
     gene            complement(7405..8079)
                     /locus_tag="VF752_02460"
     CDS             complement(7405..8079)
                     /locus_tag="VF752_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854436.1"
                     /translation="MGLLDDAIREHLELKRRHGASDEEIQQQENEALGPARREFAGAE
                     PIAPPPHEELEAEEELAYDDDELAYEDAAVGPEEPLEAEEPLAAEEPPATEERPPLPE
                     EEDTVIYEEEPAGEAEIAAPAEPPPPPPPTPPVQEQAEEPASSLPPLPDYPPAEPEDH
                     PLKDQGDKLEELLPHDRPEDVADDTEAEGEDEDVLEETPEFLQDAPEHDRLWFEQKPP
                     RDFDFD"
     gene            complement(8091..10049)
                     /locus_tag="VF752_02465"
     CDS             complement(8091..10049)
                     /locus_tag="VF752_02465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8854437.1"
                     /translation="MSFRSRLLVFFTAIVVVPMIALAFVLFSITSDAETGKADARIAG
                     GLRVAFSVYERDRGAAQSELRALVASKTTRSALASGDRTTIRRTFQEELRRHPAIESL
                     VLYDPSGRSVVQEGTQDAVAPSTAAPTTADGERLGTLAVSTTSARRYAEDVAGLTGLD
                     ARVFVGARRAASTLTETKSAEPRSGDIDVGGNSYRGRFETIREPVGPPLHVGVFDQAD
                     TLASSIDRRRIIVGGVLLAVFLAALALAIWIARSLQGEIGRFLKAARRIAGGDFEQPI
                     ETHGHDEFAALGSEFNKMSEDLARMIEEVSSKRRELEDTMRRVGEAFATGLDREGLVE
                     LAVRTAVEACEADSGRALPLDARRMNALHVGHSEKDLLDALEGAERRAFAIEADNVSE
                     LLARLEPAADEPPEQHGPTGAEVSGVHALAMPLRARLGSANDFDQVGVIAIARRGRDF
                     TETERDLFGYLASQAAVSLENANLHETVQRQAVTDELTGLFNVRHFHVTLDYEVERTR
                     RFQSEVSLVMIDIDDFKQVNDTYGHQQGDLVLIEVGRVLRELSRDIDEPARYGGEELA
                     VVLPQTDVLGAELAAERMRAAIERLEIRRVDHDGVLRVTASFGVASLPGTATTKDRLI
                     AAADQALYRAKRGGKNRVERAESAAAAR"
     gene            10177..10249
                     /locus_tag="VF752_02470"
     tRNA            10177..10249
                     /locus_tag="VF752_02470"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10210..10212,aa:Ala,seq:cgc)
     gene            complement(10632..11630)
                     /locus_tag="VF752_02475"
     CDS             complement(10632..11630)
                     /locus_tag="VF752_02475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8854438.1"
                     /translation="MKAFVVEHYGTDGLSAADLPMPEVGDADVLVKVSAASVNPLDKM
                     VRNGEFKRLLKYRTPFVLGHDVAGVVTSVGSAVRDLQVGDEVYARPRDLRIGTFAEYI
                     AIDQHDLAPKPASLTLHEAAAVPLVALAAWQALVDRAHVKPGQKVLVHAGSGGLGSTV
                     IQLAKHLGATVATTASADNAELVRSLGADEVVDYRKEHFAEVLSGYDVVLDTLGGKNL
                     EKSLTVLKPGGQAIGVAGPPDPGFAKQLGAPKLIGVAMGLLSRKVRKQARKLGVSYSF
                     LFMQANGAQLRELAALYDAGQLRPVIDKTFPFDRTLEALAYAEQGRANGKIVIALD"
     gene            complement(11627..12490)
                     /locus_tag="VF752_02480"
     CDS             complement(11627..12490)
                     /locus_tag="VF752_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8854439.1"
                     /translation="MTTVHAPTHAALSTTWKTAPTHTINAGGVEFAYRQLGPSTGVPV
                     VFLTHLAAVLDNWDPRVVDGIAAKHRVITFDNRGVGASSGTTPTTIEEMATDAVAFIR
                     ALGFEQVDLFGFSMGGMIAQVIAREEPQLVRKMIIAGTGPAGGEGIENVTRITYVDVA
                     RGVLTRQDAKQFLFFTRTPNGRRAGKEFLARLEERTSDRDKAISVRSFRAQLKAIHRW
                     GQQQPADLASIHHPVLVMNGESDRMVPTKNTVELARRLPNSELVIYPDAGHGGVFQFH
                     EDFVTRTLEFL"
     gene            12617..13195
                     /locus_tag="VF752_02485"
     CDS             12617..13195
                     /locus_tag="VF752_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8854440.1"
                     /translation="MRYSQDHKQATRQRILEAAGRRFKQDGIDGAGVATVMSDAGLTN
                     GAFYAHFTSKEDLVANVLADQLRAQRQSFDAHPSNRAGLEAFIRAYLSPQHRDEYADG
                     CPSAALLDEIARRPVATKQVFTDELMGVIDDIAARLDPTDPEAAKTDALTIFGLMLGT
                     LQLARALTDHDLSDQLLARGVETALKLLNDRA"
     gene            13858..14079
                     /locus_tag="VF752_02490"
     CDS             13858..14079
                     /locus_tag="VF752_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854441.1"
                     /translation="MGGYGYEAMASVLAAIDRAGDPLSRSSVVDAYFDGTERDSVLGT
                     YLITAERETTLDAPLAAYYVEAEALVPER"
     gene            14098..14424
                     /locus_tag="VF752_02495"
     CDS             14098..14424
                     /locus_tag="VF752_02495"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8854442.1"
                     /translation="MVVETRSKTDDVFHALADPTRRDILRRAGMDASSVSRLADVYPM
                     SFAAVQKHVVVLERAGLVTKRRRGREQLVEAAPETLAEARRALDDLEEIWRGRVERMS
                     ELLESD"
     gene            14450..14932
                     /locus_tag="VF752_02500"
     CDS             14450..14932
                     /locus_tag="VF752_02500"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8854443.1"
                     /translation="MSATTVEPDLDNLTLTVVADFEASVEEVWELWANSEKLGRWWGP
                     PEYLTTFEPYDLSAGAEMAHVMTGPDGDKHEGVWRIEAVDPPRSLELSDADVDEDGQP
                     NDGLALTGMAMELSEHNGGTRMTVRSTFFSREGMEAMAEGFAEGLRLSLSRMETLLAE
                     "
BASE COUNT         2440 a         5263 c         5199 g         2373 t
ORIGIN      
        1 gcgcgagaca caggatcatc accggcgcgc gcgcccactg ggccaggtgc tcgatcaagt
       61 cgagcatgcc ctggtccgcc cagtggatgt cctcgaaggc caggaccagc ggcgtctcgc
      121 gggccatgcc ctcgatcgac gagcggatcg cggcgaagaa cgtctcgcgg atgcgctgcg
      181 ggtcctggct ctcgccagag gcgagctcga cgggagcctc gatgccgagc aggcgaccga
      241 tcagcgcggc gcggcgctcg ccggcctcct cgccctcgcc ccagagctcg agcaggcgcg
      301 tggacagctt ctgccaggca atgtcggagg gatcgccgtc gacgatgccg cactccgcac
      361 gcagcacctc gccgagcggc cagtagacga tgctcgaccc gtagggcagg cagcgtccct
      421 ggcggaacac gtgcgggccc tcggcgccca gcgacttctc gaactcgcga agcaggcggg
      481 acttgccaac gcccgcctgg ccgacgagcg tgatgaggtg cggccgccgc tcacgccgca
      541 ctcgcccgta cagcgacttc aagaggtcga gctcatcgtc gcggccgacg agcggcgcct
      601 cgctccgtgc cagctccctg cgcaccgggt gcgcggcgag cagcccgacg acctcgaacg
      661 cctgcaccgg ctcggccttg cccttcagct cgagcggctc gagctggcgg tactcgacgg
      721 cgtgtgaggt gacgtcccgc gtgcgcggac ccaccgtgac cgtgccggga cggccggcag
      781 cctgtagtcg cgaagccacg ttgaccgtgt cgccgatcac cgtgtaggcc tcgccgaccg
      841 cgcctgcgag cacctcgccg gtgttcacgc ccacacgcag ctcgaagctg accccgtgcg
      901 agagctcgag ctcggagttg atctcgcgca tcgcgtcctg catggcgagc gcggcacgaa
      961 tggcgcgctc agcatcgtcc tcgtgcgaga ccggagcacc gaagacggcc atcacgttgt
     1021 cgccgatgta cttgtcaacg gtgccgccgt aacgctcgac ctcgttgccg aagcggcgca
     1081 ggcactggtc cacgagcgcc ttcacggtct ctgggtcgta ctgctccgca acggccgtgt
     1141 agcccgacag gtcggcgaac acgaccgtga cttggcgccg ctcctcgggc agcggcgcgg
     1201 gggcgggcgg cgcgggcacg ggcgacggtt gcgccaccga gccgtcgagc gcggttccgc
     1261 acgccatgca gaagcgggcc cccggcggcg cgggcgtgcc gcaggagggg caagcgcgtt
     1321 cgagcgccgt gccgcaggac atgcaaaagc gagcctcggc ggggttctcg aacccgcacg
     1381 ttgcgcacgc ctgcagcgcc atgtggccac gatagcccgc tgtgcggccc ttgataaggg
     1441 ggctggccac accccaaagt acgctgcgct gcgaataatg acgcgcgcgt catggaactt
     1501 cgtcagctag agcacttcgt cgcggtcgcc gaagagcggc aattcacgcg cgccgcgcgc
     1561 cgcgttcaca tcgtgcaatc cgggctgtcg gcctcgatcc gctccctgga gcaggagctc
     1621 ggcgcccgcc tcttcgaacg gcgcgcgcga cacgtcgagc tcagcgacgc gggacaggcc
     1681 ctgctcgcgg aggcgcggac ggtgctgtcc gcggccgaac gagggcgtga cgcggtggcg
     1741 gcggtccagg gcctgatgcg cggccgcgtg aacatcggct tcatgcaggg ccggacggcg
     1801 cccgagctgc cgcgagtgct cggcgagttc cacgaccggc acccggacgt cgatattcgc
     1861 ttgttccagg acgggtccgg agcgctcgcg gatgccgtgc gcagcgggcg gctggacttc
     1921 gcgtacgtct cggtgccgcg gtcggatctc gacgattgca cgtgcgcccg ctcggcgagc
     1981 tgcgcatggt gctcgccgtt tcggcccagc accgcctggc aaaccggaag gccgtcacgt
     2041 tgcaagcgct gcgagaagag acgttcgtgg acgtctcccg cgactggggc acccgcctca
     2101 tcaccgatcg cgccctcgcg gctgccgcga tcgagcgccg tgtggccttc gagctcaacg
     2161 atctgttcac ggcgatggat ctgatctccg cgggcctggc ggtcgccgtc atgccggagc
     2221 tcgagatgac tcggaacgac ggtctgaagc tcatcccgat caccggctcg cgcatggtct
     2281 gggagatctc gatggtgagc cgcgagggcg acctgagccc tgcggccgaa gagctgatcc
     2341 gtcacctgct cgaggcgaga ggcggtccgc ttcctatctc cacgagagat tgaaccgatc
     2401 gctcgttttc gttggacgga gctctcgccc gtgccgaaac tccggcacgt gaactccccc
     2461 atttggaaag taggttcccc caatgcgggc tcgtggtgag ggtgagcctg ccgcgaaccg
     2521 cacgtcgctt ctctcgcgcc gcgagcgcga ggtgctcgag caggtcgcac taggcaactc
     2581 caccaccgcg atcgcggagg agctcgtgct cagcccgcac accgtgcggg cgcacctcaa
     2641 gaacatccac cgcaagctcg agaccacgag ccgcgcgcac gcggttgctc tgctgatgga
     2701 ggaacgcggc gaggacgtgc ccaccgcggc cgggcaggat gcgcggcgaa tagtcgtgaa
     2761 ggcgctcgcc cgcgccctcg acgcgcgcga cccatatacc tcgagccaca gcgaggacgt
     2821 gaccctcctg gcgcggctgg tggcgcagga gctcgggatg agcgggaccg atgtgcgcga
     2881 tgtggaggac gtcgccgcgc tccacgacat cggcaagctc ggtgtgcccg agtcgatcct
     2941 gtggaagccg ggaccgctcg acccggtcga ggagacggtg atgcgccggc atgccgagat
     3001 cggcgccgca atcgtcgcgc acgtcgagcc aatagcgcac ctcgcgatcg cggtccgcgc
     3061 ggagcacgag cgctgggacg gcaacggcta ccccgacggc atcgcgggtg gagagatacc
     3121 gatcgcgagc cgcatcgtgc tcgcgtgcga cagctacgac gcgatggtga ccgaccgggc
     3181 gtaccgcggg cgatgaaccc gctgcgcgct cgtgaggagc tccgccgcaa cagcggctca
     3241 cagttcgacc cgcaggtggt ggacgccctg ctgggcgtgc tgcagggccg cggttgacgc
     3301 cgtctcgcca agttcgcgga cgaggcgcgc ccgatcacag gcgatagcgc cacgtgcctc
     3361 aaacgacgca ccttgcccca caagggcaca ttcgtttgat ccgcttcgac gaccgggctt
     3421 acgttcgaaa catccgccac ggaaggtccc agggatagcc acggtgcgac atcagcttca
     3481 tcgttgggca atcacggccg ctgtcgcctg cgtttgcgcc ctcgcggcgc ccggaacggc
     3541 gggtgcggct gagccaacgg tcacgcttgg cgcggccggg aacatcgcgt gcggcccact
     3601 acaggctccg agcacgagtg agtgccaaca ggacgcgacg gcctcgctca tgacatcgct
     3661 ggatcccgac gctgtagcaa cgctcggaga tcagcagtac gacagcgcga cggcgtccga
     3721 gtactcgcag ttctacgcga acagctgggg gacgatgacg gtacccgtcc acccggtgcc
     3781 cggcaaccac gaatacatgg acccggccgg caatgccgca ggctacttcg gctacttcgg
     3841 ccccgcggcg ggcaacccga gcaggggtta ctactcatac gaccttggct cgtggcacat
     3901 cattgctctc aactcggcct gcaacgtgac ccacaactgc gcacccggtt cggcccagga
     3961 gcggtggcta cgcgcggacc tcgcggcaca cccccgcgta tgcaccctcg cgtactggca
     4021 ccacccgctc ttcaccacag gggagacccg gggcgatccg aacaacgccg cgactcgctc
     4081 gttctggcac gccctctata ggcacggcgc ggacatcgtg ctcaacgccc atgaccacag
     4141 ctaccagcgc ttcgcgccac agacgccaaa cggcgcttca gacccggcgc aggggatccg
     4201 cgagttcatc gtcgggactg gcggcagaag cctctttacg ggaacggtgc cggccgcgaa
     4261 ccttcaagtc agggacatca gcaacttcgg tgtccttctg ctgacgctcg actcgggcca
     4321 ttactcatgg cggttctacc cgacccagag cggcggcttt accgactcgg ggtccgggag
     4381 gtgccacgag acgacacccg tgcttcggct gcgcgcgcac tggctgagaa tcgccgtgaa
     4441 gaccggaatg ggagccgccc gagccaagtg tgacagcgcc gcgggtgata ggtgcgttgt
     4501 gcgggtcacg ctgttcgtac gcgggaggaa ggtcggcaca ggccgcgcca cgattccggc
     4561 gggcaagctc gggatgctcc gcgtgaggtt gaaccgcaac ggccggctga tgctcagccg
     4621 cagcagaacg cacaagctgc gcgtgagagc ggtcggtgcc ccgaagaacc acgccggggt
     4681 ggcaggaccg atcgacagga cgctgatgct gaagggtatg gcgcggcggc ggcgctgagc
     4741 gcgcgtagtg agccgtggct catgaccaca acgaccgtat cgccgtgcga agccttcccc
     4801 acgcgtggtc ctcgtcaggg ccatcttcgg ccgatagcgc gatcggagga ccacgtgcgt
     4861 atacgggtcg gtgaactggt ccgacgtcat tacttcttcc gcgccccggc gcaaccggat
     4921 ggagcctgcc gggatcgaac cggcgacctc ctgcttgcaa agcaggcgct ctcccaactg
     4981 agctaaggcc cctcgcggct atggtaggcg aagggcccgc ccgggtaacg cagaaagggg
     5041 caggaggtgc tgcatcgggc ggtcccagga tggagccact gatgagatgc gtccgtgagg
     5101 gaaggagcat cgaatgacca cggaatcggc gggaacgacg gaggagcttc acggaaacgg
     5161 gcatgggccg cccgacaagt cgccccgcca ggccaacctc gcagtacgcg cgcagcgcgc
     5221 tgagatctcg aacctcgaga tcgtcaatca ctggttctcg ctcgcggccg atcggctcgg
     5281 tttgcgcgag gacatcgcgg cggtgctgcg ctcgtcctac cgcgaggtgc aggtgcagat
     5341 tccggtgaag ctcggcgacg ggaagatcca tgttttctcc ggctaccgcg tacagcacaa
     5401 cggcgcgcgc gggccctaca aaggcggcgt ccgcttccac cccgaggtgg acctcgatga
     5461 ggtgcgcgcg ctcgccgcgc tgatgacgtg gaagaccgcg gtgaccggca tcccgtacgg
     5521 cggcgccaaa ggcggcgtga acgtaccggc ctccgatctg agccagagtg agctcgagcg
     5581 catctcgcgc tcgttcatcg acaaggtgga caagatgctc ggcccgacgc gcgacatccc
     5641 cgcccctgac gtgaacacga acgctcagac gatggcgtgg atgatggatg agtacggcaa
     5701 gctgcacggg catacgccgg catgcgtgac cggcaagccg atctcgctcg agggttcata
     5761 cgggcgcgag gcggcgacgg ggcgtggctt gatctatctg ctgcgcgagg ccgctccgcg
     5821 gctcgacctc acgccgtccg ggacgcgcat cgtggtgcag ggcttcggca atgtcggctc
     5881 gtgggcggcg cggatcgcgc agcagctcgg ctgccgcatc gtgggcgttt ctgacgcgaa
     5941 cggtgcgatc gcgagtgagg ccgggatcga cgtcgaggcg ctccaccagc acatccagct
     6001 cggcgggctg atcaccgact tcgatggcgt cgaagcgatc acgccggacg agctgctcgc
     6061 gctcgagtgc gaggtgttca tccccgcggc gctcggcggc atgatccaca aggagaacgc
     6121 ggacctgctc aactgccgca tggtcatcga gggcgcaaac agcccaacga cgccggcggc
     6181 cgacgagatc ctcagcgaca agggcatcta cgtggtgccc gacgtgctcg ccaacgctgg
     6241 cggcgtgatc gtctcgtact ttgagtgggt gcagaacctg cagcacttcc gctgggacga
     6301 gcgcgaggtg aacgaccggc tcggcacgat catccgcaag gcgttccgcg aggtggccgc
     6361 gaaggcgcgc aaggagaacg acacgctccg cgtggccgcg tacgagctcg ggatcgagcg
     6421 cgtcgtggag gccgcgacta cgcgcggcta catctcgtag cggcgcctgt gtcaacgctt
     6481 atcggcgcag gcgtcggctc gcggttcatg tcgccttgac cgcttcgaag taggcgaaga
     6541 gccccgaggt gtccgtagtc gtctcttgga acccggcgtc gtcgagccag cgctggaggt
     6601 catcgaccgt tcccccggga ccaaagccgc cgcggccggg gtgcacgagc agtcggtggc
     6661 gcgcagtctg gccgcgcgtg atcatgcctc ccacgacgcg ggcgccggga cgcaggcagc
     6721 gcgccatctc cgcgaccgcg cacgaggggt ccgggaagca gtgaaggccc cagtaggaga
     6781 ggaacaggtc tacgctgctg tcctcggccg gaatcgcctg cgcgtctccc tgcacgaact
     6841 cgatctgccc tagtccgagt ctggcggcgc ggtggcgcgc ccgctcgagc atgcgggacg
     6901 agaggtcgag cgcgatgtaa cgaacatgct gggaggggcg aaggccgcgg aacgcgacac
     6961 cggcgccgca cggagcatcg acgatcaagc cgccatcggg caccgctcca atcgcgcgca
     7021 tgctcgcgta gaaggggcgg ctgtcggaac cccacacaag cgcggcgatc aggcgtgaga
     7081 gccgctcgcg ctcgatgtag aggccgtaga ccgcgccgaa ggcgccgtcg aagagcgccc
     7141 ggttccgctc acgcgcgtta tcgccggtgg ccgggttcgg cacaccgcga agtatctccc
     7201 ggcgcgcgag aagcaaccca tggcgagcgg ggaccgcatg ccccgcagtc cccgcccgcc
     7261 cacaggtcgg cggcatcgtg ccgcttccaa cgacggaacc ctccatgccg tcgtgcccct
     7321 tttatcgtcc gcgcgcgcgc acccacgcag ggcttctcgc gcaaaaccga tcaaacgtcc
     7381 aggcgccctc caggggcgag gtgatcagtc gaagtcgaag tcgcgcggcg gcttctgctc
     7441 gaaccagagg cggtcgtgct ccggcgcgtc ctggaggaac tccggcgtct cctcgagcac
     7501 gtcctcgtcc tcgccttccg cttcggtgtc gtccgcgacg tcctccgggc ggtcgtgcgg
     7561 gagaagctct tcgagcttgt cgccctggtc cttgagcgga tggtcctcgg gctcggccgg
     7621 ggggtagtcg ggcagcggcg gaaggctgct cgccggctcc tcggcctgct cctgcaccgg
     7681 cggcgttggc ggcggtggcg gaggcggctc cgccggcgcc gcgatttcgg cctcgccggc
     7741 gggctcctcc tcgtagatca cggtgtcctc ttcctcggga agcggcgggc gctcctccgt
     7801 cgccgggggc tcctctgccg caagcggctc ctccgcctcc agcggctcct ctgggcccac
     7861 ggccgcgtcc tcgtaggcga gctcgtcgtc gtcgtaggcg agctcctcct cggcctccag
     7921 ctcctcgtgc ggaggcggcg cgatcggctc cgcgccggcg aactcgcgtc gcgcgggccc
     7981 gagcgcctcg ttctcctgct gctggatctc ctcgtcgctc gcgccgtggc ggcgcttcag
     8041 ctcgaggtgt tcgcgaatcg cgtcgtcgag tagccccatg tcgccacagg ctaccgagcc
     8101 gcggccgcag actccgcgcg ctccactcgg ttcttgccgc cgcgtttcgc gcggtagagc
     8161 gcctggtccg ccgccgcgat cagccggtcc ttggtcgtcg cggttccggg cagcgacgcc
     8221 acaccgaagc tcgccgtcac ccgcagcacg ccgtcatggt ccacgcgccg gatttcgagc
     8281 cgctcgatcg ccgcgcgcat acgctcggcg gcgagctccg cgcccaatac gtctgtctgt
     8341 ggcagcacca cggcgagctc ttcgccgccg taacgcgcag gctcgtcgat gtcgcgcgag
     8401 agctcgcgta gcactcgtcc cacctcgatc agcaccaggt cgccctgctg gtggccgtag
     8461 gtgtcgttca cctgtttgaa gtcgtcgatg tcgatcatca cgaggctcac ttcgctctgg
     8521 aagcgccgag tgcgctccac ctcgtagtcg agggtcacgt ggaagtggcg cacgttgaag
     8581 aggcccgtga gctcatccgt gaccgcctgg cgctgcaccg tctcgtggag gttcgcgttc
     8641 tcgagcgaca cggcggcctg gctcgcgagg taaccgaaca ggtcgcgctc ggtctcggtg
     8701 aagtcccggc cgcggcgcgc gatggcgatc accccgacct gatcgaagtc gttggcagat
     8761 ccgaggcgcg cgcgcaacgg catcgcgagc gcgtggacgc ccgacacctc cgcccccgtg
     8821 ggcccgtgct gctccggagg ctcgtcggcg gccggctcga gccgcgcgag cagctcggac
     8881 acgttgtcgg cctcgatggc gaacgctcgc cgctcggcgc cctcgagcgc gtcgagcaga
     8941 tccttctcgc tgtggcccac gtgcagcgcg ttcatcctgc gcgcgtcgag cggcagcgcg
     9001 cggccactgt ccgcctcgca ggcctcgacc gcggtccgca ccgcgagctc gaccaggcct
     9061 tcacggtcga gaccggtcgc gaacgcctcg cccacccggc gcatagtgtc ctcgagctcg
     9121 cgcctcttgc tcgagacctc ctcgatcatg cgcgcgaggt cctccgacat cttgttgaac
     9181 tcgctgccga gcgccgcgaa ttcgtcgtgg ccgtgcgtct cgatgggctg ctcgaagtcg
     9241 ccgcccgcga tgcgccgcgc ggccttgagg aagcggccaa tctcgccctg tagcgagcgc
     9301 gcgatccaga tcgcgagggc gagcgcggcg aggaacaccg cgagcaggac gccgccgacg
     9361 atgatgcgcc gccgatcgat cgagctcgcc agcgtgtcgg cctgatcgaa gacgcccacg
     9421 tggagcggcg gcccaaccgg ttcccggatc gtctcgaaac gaccgcgata gctgttgccg
     9481 cccacgtcga tgtcgccaga gcgcggctcc gcggacttgg tctcggtgag cgtggaggcc
     9541 gcgcgccggg cgcccacgaa cacccgcgcg tcgaggccgg tgagccctgc gacgtcctcc
     9601 gcgtaccgcc gcgcactcgt ggtcgagacg gcgagcgtcc cgagccgctc gccgtcagcc
     9661 gtcgtcggcg cggcggtcga gggcgccacc gcgtcctgcg tgccctcctg cacgacggaa
     9721 cgcccgctcg ggtcgtatag aaccagcgac tcgatcgcgg gatgccggcg cagctcctcc
     9781 tggaaggtcc ggcggatggt cgtccggtcg ccgctcgcga gcgcgctgcg agtggtcttg
     9841 ctcgcgacga gcgcccgcaa ctccgactgc gccgcgccgc ggtctcgctc gtacaccgaa
     9901 aacgcgaccc tcaggccccc ggcgatgcgc gcgtccgcct tgccggtctc ggcgtccgag
     9961 gtgatcgaga acagcacgaa agcgagcgcg atcatcggta cgacgacgat cgccgtgaag
    10021 aagacgagta gccggctcct aaagctcatc cttggcgcgc gccccttgac gtggggtacc
    10081 catcctgaac ggttcgtcaa tctcgtgcat ttgtccgttc gagcgtcacg tctgccgcta
    10141 ccatggtgcc ccggtcgcgc ttcgcgcgcg tcacacgggg ctatagctca gttgggagag
    10201 cgcctggatc gcacccagga ggtcgggggt tcgagtcccc ctagctccat ctcacgaatc
    10261 catgtaacca tgccgtttcg cgcctctcgg gctcagtcct gatgtgtccg cgggcgatgg
    10321 tcgttggccg gtctttggcc cgatctccgt gctacgcgtc gtgccccact agctggaaag
    10381 ccccatttca agccatttcg gccggaagga gcggctcagg ccgggggact aatagaactc
    10441 catctaggag cgctcgggtt cgagttcgcg ggtcgcgcgc ctgccgcggc gcgcgctccg
    10501 ttcccggccg cgggagacgt gcacgagcgg gttcacgcct cctgattgtc gtcgaagaca
    10561 gggcgtctgc ggcgccgcca ttcctttgcg cttctggacc cattgcaggc gttgggcggc
    10621 agggggcgtt gtcagtcgag cgcgatgacg atcttgccgt tggcgcggcc ctgctcggcg
    10681 tacgccagcg cctcgagggt ccggtcgaac ggaaacgtct tgtcgatgac cgggcggagc
    10741 tggccggcgt cgtagagggc ggccagttcg cgcagctggg cgccgttggc ctgcatgaac
    10801 aggaacgagt agctcacgcc gagcttgcgg gcctgcttgc ggaccttgcg gctcagcagc
    10861 cccatcgcga cgccgatgag cttcggcgcc ccgagctgtt tcgcgaaccc ggggtccggc
    10921 gggccggcga cgccgatcgc ctgtccgccg ggcttgagga ccgtgagcga cttctcgagg
    10981 ttcttgccgc ctagcgtgtc cagcacgacg tcgtagcccg agaggacttc ggcgaagtgc
    11041 tccttcctgt agtccacgac ttcgtcggcg ccgaggctgc gcacaagctc cgcgttgtct
    11101 gcgctcgcgg tcgtcgccac cgtggcgccg aggtgcttgg cgagctggat gacggtcgac
    11161 ccgaggccgc cggagccggc gtggacgagg accttctggc cgggctttac gtgggcgcgg
    11221 tcgacgagcg cctgccaggc ggccagcgcc accagcggca ccgccgccgc ctcgtggagg
    11281 gtgagcgagg ctggcttggg ggcgaggtcg tgctggtcga tcgcgatgta ctccgcgaac
    11341 gttccgatcc gcaggtcgcg gggacgggcg tagacctcgt cgcccacctg gaggtcgcgt
    11401 acggcggagc cgacgctggt caccacgccg gcgacgtcgt ggccgagcac gaacggggtc
    11461 cggtacttca ggagccgctt gaactcgccg ttgcggacca tcttgtccag cgggttgacg
    11521 ctggccgcac tgaccttgac cagcacgtca gcgtcgccga cctctggcat tggtaggtcg
    11581 gcggcggaca gtccgtccgt gccgtagtgc tcgacgacga acgccttcat aggaactcga
    11641 gcgtccgcgt gacgaagtcc tcgtggaact ggaacacgcc gccgtgcccg gcgtcgggat
    11701 agatcacgag ttcgctgttc gggagtcggc gggcgagctc gacggtgttc ttggtgggca
    11761 ccatcctgtc gctctcgccg ttcatgacga gcaccggatg gtggatgctc gcgaggtcgg
    11821 cgggctgctg ctggccccat cggtggatgg ccttgagctg ggcgcggaat gagcgcacgg
    11881 agatggcctt gtcgcggtcg ctcgtacgtt cctccagccg tgccagaaac tccttgccgg
    11941 cgcggcgccc gttcggggtt ctggtgaaga agagaaactg cttggcgtcc tggcgggtga
    12001 gcactcctcg cgcgacgtcg acgtaggtaa tgcgcgtcac gttctcgatt ccttcgccgc
    12061 ccgcggggcc ggttccggcg atgatcatct tgcggaccag ctgcggctcc tcccgcgcga
    12121 tcacctgggc gatcatgccg cccatcgaga aaccgaacag atcgacctgt tcgaagccga
    12181 gcgcgcggat aaaggcgacg gcgtccgtgg ccatctcctc gatggtcgtc ggcgtggtcc
    12241 cgctggaggc accgacaccc cggttgtcga aggtgatgac ccggtgcttg gcggcgatgc
    12301 cgtccacgac tcgggggtcc cagttgtcca ggaccgcggc caggtgggtg aggaagacca
    12361 ccggcacccc ggtgctcggg ccgagctgac ggtaggcgaa ctcgacgccg ccggcgttga
    12421 tggtgtgcgt cggcgcggtc ttccacgtcg tgctcagagc cgcatgggtg ggggcgtgga
    12481 cagttgtcat ggcgtttgct ccttgctgca tcggctttgc attacggtcg taatactata
    12541 gtacgatcat aatgcaatag cgagacgcga agacgtctca cgacaggatc aagagcagtt
    12601 cggcgagaga ggtcgcatgc gctacagcca ggaccacaag caggccacga gacaacgaat
    12661 cctcgaagcg gccgggcggc gcttcaagca agacggcatc gacggcgcgg gcgtggccac
    12721 cgtgatgtcc gacgccggcc tgaccaacgg cgccttctac gcccacttca cgtccaagga
    12781 agacctcgtc gcgaacgtgc tcgccgacca gctgcgcgcc caacgccaaa gcttcgacgc
    12841 gcacccctcg aatcgggccg gactcgaagc gttcatccgc gcctacctct ccccgcaaca
    12901 ccgcgacgag tacgccgacg gctgcccatc ggccgcgctg ctcgacgaga tcgctcgccg
    12961 ccccgtcgcc accaaacagg tcttcaccga cgaactgatg ggcgtcatcg acgacatcgc
    13021 cgcgcgcctc gacccgacag accccgaggc ggctaaaacc gacgcgctca cgatcttcgg
    13081 actaatgctc ggcaccctgc agctcgccag ggcgctgaca gaccacgacc tctccgatca
    13141 actcctcgcc cgcggagtgg agacggcctt gaagttgctg aacgaccggg catgaccacg
    13201 tcgccacaca ctccccgccc ccggcaacga cgacaaccag ggctgggctg tcgcgtcgcc
    13261 gactcgcacg gctgacggga ctgagcacgc tcccgtcggc acggaccgga ttgagatccc
    13321 ggtggagctc gcgggctcgg tgctcagcgt ggtacgccgc acgcctctca cgactaccgc
    13381 ctcgattcga gcccctgaag ccaccgctcc ccctgtctct tacaccgggc actccggctt
    13441 ccgcggggtc ccacccccaa gtgcgttgcc gctcccgccc gcatgcgtgc atctccgctt
    13501 cggcgctgca gacgcccgca agagacggtt ggccgtagct cgatcagacg ggagatcggg
    13561 ccaaagaccc gcttctttgg cccgatccca gccgaaatgg gcggaaacca gcggaaaatg
    13621 cgggccacag acctggcgtc ggtcgccccg aaacccctgt aaacaagcca aattcagggc
    13681 tgggggtcgc ctggatcgca ctcaggaggt cgccggttcg agtccggcta gctccactcc
    13741 gagaacccca gcaagcgctg gggttctcgc ttttcgtggg cgagttgcgg gcgtcgaatt
    13801 tcatcatgcg aacgttgaaa tggcgcgaaa cgtgatgaac gggcgcgagc ctgggccgtg
    13861 ggcggctacg gctacgaggc catggcgtcc gtcctcgctg ccatcgatcg cgccggcgat
    13921 cccctctcgc gctcctccgt cgtcgacgcc tacttcgacg gcacggagcg cgactcggtc
    13981 ctcggcacct acttgatcac cgccgagcgg gagacgacgc tcgatgcgcc cttggccgcc
    14041 tactacgtgg aagcagaggc gctcgttccg gagcgatagc tgctaatcta caaccaaatg
    14101 gttgtagaaa cgagaagcaa gaccgacgac gtcttccacg ctctcgccga cccgacgcgg
    14161 cgcgacatcc tgcggcgcgc agggatggac gcatcatccg tctcgagact cgccgacgtg
    14221 tacccgatgt cgttcgcggc cgtgcagaag cacgtcgtcg tgctagagcg cgcgggactc
    14281 gtcacgaagc gccggcgtgg ccgcgagcag ctcgtcgagg ccgcacccga gacgctcgct
    14341 gaggcacggc gagcgctcga cgacctcgaa gagatctggc gtggccgggt cgagcggatg
    14401 tccgagctcc tcgaatccga ctgacaacac ccaacgaaag gaagcctcca tgtccgccac
    14461 caccgtcgaa cccgacctcg acaacctgac cctcacggtc gtcgccgact tcgaggcgtc
    14521 ggtcgaagag gtgtgggagc tttgggctaa ctcggagaag ctcgggcgct ggtggggacc
    14581 gccggagtat ctgacgacct tcgagcccta cgacttgagc gccggcgccg agatggccca
    14641 cgtgatgacg ggtcccgacg gcgacaagca cgagggcgtg tggcggatcg aagcagttga
    14701 tccgccgcgc tcactcgagc tgagcgacgc cgacgttgac gaagatggcc aaccaaacga
    14761 cggcctcgcg ctcacgggaa tggcgatgga actctcggag cacaacggcg gcacccggat
    14821 gacagtgcgc tccacattct tctcgcgcga ggggatggag gccatggcag agggcttcgc
    14881 cgaaggcctc cggttgtcgc tcagccggat ggagacgctc ctcgcggagt agggccagcg
    14941 gtccaagagg tccctgagcg cgggctcgag ctcggggaac cggaacgtgt agcccttgtc
    15001 gagcgcccgg cgcggaacca ctcgcaggct ggccgcgacg aagtcggcga cttcgccgcc
    15061 acccatcgcc gtcaccgcga acttcggcgc cgggaacaac gccgggcgct tcagcacctt
    15121 gccgaggagc ttcgagaacg tcttgttcgt caccgggttt ggcgccgagg cgttgaccgg
    15181 gtccgagatc ccctccgtaa accgatccac catacggcca acgccgcggc gccgccggcg
    15241 aagatgagga cagggacgag ccgccgggcg cgctc
//
LOCUS       DASVXS010000047         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_10695, whole genome shotgun sequence.
ACCESSION   DASVXS010000047 DASVXS010000000
VERSION     DASVXS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10695"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF752_02505"
     CDS             complement(<1..218)
                     /locus_tag="VF752_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854444.1"
                     /translation="MRPRALVFLTAVLSLGTASAAHAAPKQAIFDVKAKGHYFADRVV
                     DTQPGSFCVKERGHTEDHKRLDVTFHMR"
     gene            316..2346
                     /locus_tag="VF752_02510"
     CDS             316..2346
                     /locus_tag="VF752_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854445.1"
                     /translation="MQFRGIARFGAALAVLAAALAAATASAAGPLPIVDPATPAARDT
                     EPVVLTGRDFGDWSARSNVTFKIPLTDLEDCQTFDGQCAHNHYATPEVDSQQWAPQEG
                     TPVGRLLGYRWNPRANKFQQIPFQVDEVFTRYLDNSASGFSVYSGEDQHSTYSYDREG
                     FRYTKSDPSNPCLAQPDSPAMKDPVRGLDDNDELAFMASDAGPQAPAGAKLPKGVEAA
                     RTITLTDPSNPGAAPTYVYVMKAGDHGPKPAFDATNGYVKYERDANAVLFEKSESSYD
                     GYGNAATGPYCDANGNLVTNPDGTPKLDRRRPRDYAWVKTPRYQFRYDGRWLMTQLRI
                     SPDGGESYGPDLIDRWKARAFAQDPGSETPCCGFEEEDTNWGGSSTLLGERVGPVRAM
                     RETWGADSGTNVIRRETFYRDEVRQKSWLRVHVIPPLDGIYAQWDFNAGRVNRFYNSE
                     KQDGVPVDGQNDEIYGNFDDPCNERYDQNGTGSLDQGFRATYRAAQICQISPYHQSVD
                     SPDLTFSAANAALAWSEVAGPWGTIVDRISTSATDLTPGGAAQSLLAVPYYRDDSCFD
                     DGTGSDPGPKVRLRSGDEPATASDGSPRKCWQPSDGIPDGSDHFYQGSIATHGVHILA
                     IADSDNARQTVPVDEIVSDWRMVALPGDPGNVGEKYGRGFEKPLLATVQPGG"
     gene            2376..2819
                     /locus_tag="VF752_02515"
     CDS             2376..2819
                     /locus_tag="VF752_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX8854446.1"
                     /translation="MDYEAVKEGLAQAIPYNRHIGLELIEVGDGRGVVRLPDEDGLHN
                     HVGSQHAGGLFSAGEFASGGAVIGALAEHMAGITPLAKGAEIRYLKLAKGPITATGTL
                     GEDKAELLSKVESDGRVEFPVNVEMQDGDGQKVAEMTVHWHLRKN"
     gene            2831..3511
                     /locus_tag="VF752_02520"
     CDS             2831..3511
                     /locus_tag="VF752_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX8854447.1"
                     /translation="MNVGAVLAGTLVGTLIGSRLPEGLQQRVMAGLGLVTLVIGVDLA
                     LAWRDTNPLYVLGGVLLGGLVGEAIGIEAWLARLGDRVQRSLAGHRHSTISEAFVTAS
                     LLFCVGPLTVVGAIQDGLTGDYQALATKSMLDGFASIALSATLGWGVALAAGTVLVVQ
                     GAISLGAGLFKDILQGEALDAMTSAGGILIIGISLKLLNLKDVKVGNFLPALIIAPGL
                     VGIVSLFG"
     gene            complement(3508..>3743)
                     /locus_tag="VF752_02525"
     CDS             complement(3508..>3743)
                     /locus_tag="VF752_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854448.1"
                     /translation="ELYPKPPPVPPASAEDAARVHFSYVIPRVGAAASTHMFNPCLPA
                     ISDACMKHPPGDADFAPFKDKSRQPDLLPANNY"
BASE COUNT          623 a         1266 c         1312 g          542 t
ORIGIN      
        1 atgcgcatat ggaaggtcac gtcgaggcgt ttgtggtcct cggtgtggcc gcgctccttg
       61 acgcagaagc tgcccggctg ggtatcgacg acgcggtcgg cgaagtagtg gcccttcgcc
      121 ttcacgtcga agatcgcctg cttgggggcc gcgtgggccg cggaagcggt gcccaggctg
      181 agcacggcgg tgaggaaaac aagagccctt ggacgcatag gggtacgccg aagctacatc
      241 cggtgtacgc gtttccgcaa caaccggcgc gcgccaacgt gcgagccgtt acaacatccg
      301 gcgggggtac gtgcgatgca gttccgagga atagcgcgct tcggcgccgc actggctgtg
      361 ctcgccgctg cgctcgcggc ggcgacggcc tctgcggctg gtccgctgcc gatcgtggat
      421 cccgcgacgc ccgccgctcg cgacaccgag ccggtcgtgc tcaccggacg agacttcggc
      481 gactggtcgg cgcgctcgaa cgtcaccttc aagatcccgc tcacggatct ggaggactgc
      541 cagaccttcg acgggcagtg cgcgcacaac cactacgcga cgcctgaggt cgacagccag
      601 cagtgggcgc cgcaggaggg cactccggtc ggtcgcctgc tcggctaccg ctggaacccg
      661 cgcgcgaaca agttccagca gatcccgttc caggtggacg aggtcttcac gcgctacctc
      721 gacaactcgg cgtcgggctt ctcggtctac tcgggcgagg accagcacag cacgtactcc
      781 tacgaccgcg agggcttccg ctacaccaag tccgacccgt cgaacccgtg cctcgcgcag
      841 cccgactcac cggcgatgaa ggacccggtg cgcggcctcg acgacaacga cgagctcgcc
      901 ttcatggcga gcgacgcggg tccgcaggcg cccgccgggg cgaagctgcc gaagggagtc
      961 gaggcggctc gcacgattac gctcaccgat ccgtcgaatc ccggcgccgc gccgacgtac
     1021 gtgtacgtga tgaaggcggg tgaccacggg ccgaagccgg cgttcgacgc caccaacggt
     1081 tacgtgaagt acgagcgcga cgcgaacgca gtcctattcg aaaagtcgga gtcgagctac
     1141 gacggatacg gcaacgccgc cacgggcccc tactgcgacg cgaacggcaa cctcgtcacg
     1201 aacccggacg gcacgccgaa gctcgaccgc cggcgcccgc gcgactacgc ctgggtcaag
     1261 acgccgcgct accagttccg ctacgacggc cgctggctga tgacccagct ccgcatctcg
     1321 cctgacggcg gcgagagcta cggccccgac ctgatcgacc gttggaaggc gcgcgccttc
     1381 gcgcaggacc cgggctctga aacgccctgc tgcggcttcg aggaggagga cacgaactgg
     1441 ggcggctcga gcacgctcct cggcgagcgc gtcggcccgg tgcgggcgat gcgcgagacc
     1501 tggggcgccg actctggcac gaacgtgatc aggcgcgaga cgttctaccg ggacgaggtg
     1561 cgtcagaaga gctggctgcg cgtgcacgtg atcccgccgc tcgacggcat atacgcccag
     1621 tgggacttca acgcggggcg cgtgaaccgc ttctacaact ccgagaagca ggacggcgtg
     1681 ccggtagacg gccagaacga cgagatctac ggcaacttcg acgacccctg caacgagcgc
     1741 tacgaccaga acggcacggg cagcctcgac cagggcttcc gggcgacgta tcgcgccgcg
     1801 cagatctgcc agatctcgcc ctaccaccag tcggtcgatt cacccgacct cacgttctcc
     1861 gcggcgaacg cggctttggc gtggtccgag gtcgcgggcc cgtggggcac gatcgtcgac
     1921 cgcatctcca cgagcgcaac cgacctcacg cccggcggcg ccgcgcagtc gctgctcgcg
     1981 gtgccctact accgcgacga ctcgtgcttc gacgacggca ccggctcgga tcccgggccg
     2041 aaggtgcgcc tgcgctcggg cgacgagccc gccaccgcgt cggacggctc gccgcgcaag
     2101 tgctggcagc cgagcgacgg gatccccgac gggtctgacc acttctacca gggcagcatc
     2161 gccacccacg gcgtgcacat cctggctatc gcggactccg acaacgcgcg acagacggtg
     2221 ccggtcgacg agatcgtgtc ggactggcga atggtggcgc tgccgggcga cccgggcaac
     2281 gtcggcgaga agtacgggcg cggcttcgag aagccgctcc ttgcgaccgt tcagccgggc
     2341 ggctgagccg gcgcggcccg atagggtgcc gggccatgga ctacgaagcg gtcaaggagg
     2401 gcctggctca ggccattccc tacaacaggc acatcgggct cgagctcatc gaggtcggcg
     2461 acggccgggg cgtcgtgcgc ctgccggacg aggatggcct gcacaaccac gtcggctcgc
     2521 agcacgccgg cgggctcttc tcggccggcg agttcgcgtc aggcggcgcc gtgatcggcg
     2581 cgctcgcaga gcacatggcc gggatcacgc cgctggcgaa gggcgccgag atccgctatc
     2641 tcaagctcgc gaagggtccc atcacggcaa ccggcaccct gggcgaagac aaggcggagc
     2701 tgctctcgaa ggtcgagtcg gacggacgcg tggagttccc ggtgaatgtc gagatgcagg
     2761 acggtgacgg gcaaaaggtg gctgagatga ccgttcactg gcacctgcgg aagaactgac
     2821 cggcaccttc atcaacgtcg gggccgtgct ggcgggcacg ctcgtcggca cgctcatcgg
     2881 gagccgcctc cccgaggggc tgcagcagcg cgtgatggcg ggcctcggcc tcgtcacgct
     2941 cgtcatcgga gtggacctcg cgctcgcctg gcgcgacacg aacccgctct acgtgctcgg
     3001 cggcgtgctg ctcggcgggc tcgtgggcga ggcgatcggg atcgaggcat ggctcgcgcg
     3061 cctcggcgac cgcgtacagc gcagcctcgc cgggcatcgc cactccacga tcagcgaggc
     3121 gttcgtgacg gcgagcctgc tcttctgcgt ggggccactc acggtggtag gcgccatcca
     3181 ggacgggctg accggcgact accaggcgct cgcgaccaag tcgatgctcg acggcttcgc
     3241 gtcgatcgcg ctgtcggcaa cgctcggctg gggcgtggcc ctcgccgccg gcacagtgct
     3301 ggtcgtgcag ggcgcgatct cgctgggcgc aggcctgttc aaggacatcc tgcagggcga
     3361 ggcgctcgac gctatgacga gcgccggcgg gatcctcatc atcgggatct cgctgaagct
     3421 gctcaacctg aaggacgtga aggtcggcaa cttcctcccg gcgctgatca tcgcaccggg
     3481 gctagtgggg atcgtgtccc tgttcggcta gtagttgttc gcgggaagca ggtccggctg
     3541 gcgcgacttg tccttgaagg gagcgaagtc ggcgtcacca ggcgggtgct tcatgcaggc
     3601 atcggagatg gcaggcaggc acgggttgaa catgtgcgtg ctagctgcgg ccccaactcg
     3661 cgggatcacg taagagaagt gcaccctggc cgcatcctca gcggaggccg gcggcacggg
     3721 cgggggcttc gggtagagct cct
//
LOCUS       DASVXS010000048         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_931765, whole genome shotgun sequence.
ACCESSION   DASVXS010000048 DASVXS010000000
VERSION     DASVXS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931765"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1534)
                     /locus_tag="VF752_02530"
     CDS             complement(<1..>1534)
                     /locus_tag="VF752_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854449.1"
                     /translation="VTADELLPSLKRTHPPRMLITTDGRHFRAVPARGVLLRNPFGTN
                     RVIGFRTLKVWRDRLYLTLSAGLTGDGEVYEVTRPWGPNARFRQITPKDLAVFEIEKF
                     GNALYAGTGSRTTGYGVYKTSSRKAPYKWTPVVTNAAGRGARITSVVSMYPFEGRLYV
                     GASGWYNDEIPQSELISIASSGSWEVVVGAPREVNGVLKAPISGLGDGFNSIFAAHFW
                     RMELEDGGLFLGTNDWSWALQADTQWWPLLSGVLWWELGFDLWGTCNGREWFPVTRNA
                     FNSDVFNFGARNLVRTPYGLFVGSANHAEGTSVWLDRQQPCGAPVGNAARAPRSPQQV
                     LTDVQRDGTVVSWAAAARADRYRVLRAKQIPVSFSVKSPPALPNGFRLDTELPEPVPA
                     GMPGSAAVDGTVLGAFRPIGTTASRHFVDRSRKLGVGYAYRVIAVGSNGQSQPSGVQF
                     VPDLRPRVTWAALERAVGRASQAARVRAAATLLRHGHRQRSLASLERVRRSARSGSAL
                     EDL"
BASE COUNT          241 a          546 c          511 g          236 t
ORIGIN      
        1 cgaggtcctc gagcgcactc cctgaccgtg cgctgcgccg cactcgctca agcgacgcga
       61 gcgagcgctg gcgatggccg tgtcgcagga gagtcgcggc ggcccgaacg cgtgcggcct
      121 gcgatgccct gccgacggcg cgctctaggg ccgcccacgt gaccctcggc ctcaggtccg
      181 gaacgaactg cacgccggac ggctgcgact gtccgttcga ccctacggcg atgacccggt
      241 aggcgtagcc gacaccgagc ttccgcgagc gatcgacgaa gtgccggctc gccgtagtgc
      301 cgatcgggcg gaacgcgccc agcacggtgc cgtcgacggc tgcactcccc ggcatgcccg
      361 caggcaccgg ctcgggcagc tccgtgtcga ggcggaagcc gttgggcagc gctggcggcg
      421 acttgaccga gaaggacacg gggatctgct tcgcgcgaag cacgcggtag cgatcggcgc
      481 gcgcggcagc cgcccacgag acgaccgtgc cgtcacgctg cacgtccgtc agcacctgtt
      541 gcggtgaccg cggcgctctc gcggcatttc ctacgggtgc gccgcacggc tgctggcggt
      601 ccagccagac ggacgttccc tccgcgtggt tcgcgctacc cacgaagagc ccgtacggag
      661 ttcggacgag gttccgagca ccgaaattga agacgtccga gttgaaggcg ttgcgggtca
      721 ccgggaacca ctcccggccg ttgcaggtcc cccagaggtc gaagccgagc tcccaccaga
      781 gcacgccgct cagcagcggc caccactgtg tgtcggcctg caacgcccag ctccagtcgt
      841 tcgttccgag gaagagccct ccgtcctcga gctccatccg ccagaagtgg gcggcgaaga
      901 tgctgttgaa cccgtctccg agaccgctga tgggcgcctt caggacgccg ttcacctcac
      961 gcggcgcgcc gacgacaacc tcccagcttc cggacgaagc gatgctaatc agctccgact
     1021 gcgggatctc gtcgttgtac cagccactcg ctccgacgta gagccggccc tcgaacggat
     1081 acatcgacac gacagacgtg atgcgcgcgc ctcggccggc ggcgttcgtc accaccggag
     1141 tccacttgta aggcgccttg cggctactcg tcttgtagac gccgtacccg gtcgttctgc
     1201 tgccggtccc tgcgtagagc gcgttcccga acttctcgat ctcgaagacc gcgaggtcct
     1261 tcggcgtgat ctgcctgaag cgcgcgttcg gcccccacgg tcgcgtgacc tcgtacacct
     1321 cgccgtcgcc ggtgagaccg gcgctcaggg tgaggtagag ccggtcgcgc cagaccttca
     1381 gcgtccggaa cccgatgacc ctattggtgc cgaagggatt tcgcagcagc accccgcggg
     1441 ccggtacggc ccggaaatgc cgcccgtccg tggtgatgag catgcgcggc gggtgcgtgc
     1501 gcttgagcga gggcaggagc tcgtccgccg tcac
//
LOCUS       DASVXS010000049        18604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1144499, whole genome shotgun sequence.
ACCESSION   DASVXS010000049 DASVXS010000000
VERSION     DASVXS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18604
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144499"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF752_02535"
     CDS             complement(<1..402)
                     /locus_tag="VF752_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8854450.1"
                     /translation="MPSSKPSPLLAPLVAALLLAPVAEASAATPRTAALQVALTGLHL
                     YRGSIDGIAGPATRRAVRRLQRRRHLAVDGVAGPRTRRALGWRGRPDLGRRQLRRGRR
                     GWDVSELQFLLRRRGFSPGSIDGGFGRATRSA"
     gene            501..1247
                     /locus_tag="VF752_02540"
     CDS             501..1247
                     /locus_tag="VF752_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8854451.1"
                     /translation="MAEELSGKVVAITGASSGIGEATALACSWAGAAVSLAARRLDRI
                     EELARRIKYEGGRALAIETDVGDEDQANAFVERTNEELGRLDVLVNNAGVMLLGPIEN
                     APTEEWRRMIHANVFGVIYCTHAALPIMRAQSAGHIVNVSSVAGRVARAGAGVYNLTK
                     FGVGAFSEALRQEVTASGIRVTLVEPGAVATELGSHNRPEILEALRSDLGSVTRMDAG
                     DIANAILYTITAPENVSVNEMLIRPTGQVR"
     gene            complement(1240..2193)
                     /locus_tag="VF752_02545"
     CDS             complement(1240..2193)
                     /locus_tag="VF752_02545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX8854452.1"
                     /translation="MRIGYFLSCEQFGPRELVDKARRAEEAGFQDLWISDHYHPWNDE
                     QGQSPFVWTVIGQLAEATSRMRVTTAVTCPTIRIHPAVIAQAAATATVALDGRFNLGV
                     GTGENLNEHILGDHWPEAEERIEMLEEAIEVMRMLWQGGTRSHRGRHYRVENARIYTL
                     PPEPPPVVVSGFGRRSVELAGRVGDGYAITMPDRDLISHFHSAGGQGKPVQAGAKVCW
                     DEDEQRAIDEVHRLWPNEVLPGELAQVLPTPAHFEQATELVTKEMVAESVPCGPDLDK
                     HLQALQQYADAGADELYVQQIGPDLEGFFSTYSREVLPKFT"
     gene            2297..2653
                     /locus_tag="VF752_02550"
     CDS             2297..2653
                     /locus_tag="VF752_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854453.1"
                     /translation="MSTQDDDGHAISYQALRRGTPVRAADGTEVGTVRKVLENTRENI
                     FDGIVVETHKGRRFVDAPEVARVAERAVTLTISAEDVDALPAPRSVMKDRWDQATLVR
                     RARRAGRDARRRWQNR"
     gene            complement(2658..2867)
                     /locus_tag="VF752_02555"
     CDS             complement(2658..2867)
                     /locus_tag="VF752_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854454.1"
                     /translation="MVLAISTLGVIVLVVAVVLVGAVVMAVVFRGSKREPQVEHEQEV
                     VLEEQFGSGEPVDWQGDLKPPRDPY"
     gene            complement(2899..3345)
                     /locus_tag="VF752_02560"
     CDS             complement(2899..3345)
                     /locus_tag="VF752_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854455.1"
                     /translation="MSRARGILVAMDDDELRAVLRSGYEALSRGDVDAVLALLADDVE
                     LRDRAEAPEAGVYHGHDGARVAFQQTDDTFENYRIRPTAIELGPVRDGGIRNVVVVLR
                     QEGEGRLTGVRVSEDIAHVLTLKDDVAIRLRVFSTPDEARAASLES"
     gene            3395..4729
                     /locus_tag="VF752_02565"
     CDS             3395..4729
                     /locus_tag="VF752_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8854456.1"
                     /translation="MTDVAVEAPPSAIPPGPRALPLANLYAWLSRPIAMLERCQRRYG
                     DVFAMDFGEKHGRFVFVADPGLIREVFTEQRDSFVAGAPNSVLSPIVGTGSVLTTDGA
                     RHLRQRKLLLPPFHGERLEAYRSRIRGLAEQTVAGWPENRTFAVADSFQRMTLEVIVD
                     VVFGVSDEERRRRLLEMLPPLTRSATTMIFFPVLERDLGPLSVGGRVRRLREAVDELI
                     HTEIAAHRTAEREGEPRGDILAMLIAARDEEGEALSDQELRDELMTLLFAGHETTATA
                     LAWSVDLILQHPRVHGRLVEDLRTNGDEYLDAVIQETHRFRPTVPNVVREATRDVELG
                     DWLIPAGDVVAPAIHLVHRRPDLYPEPDVFRPERFLDSRAETYGWLPFGGGIRRCIGA
                     SLAQLEMRETLRVVFERVQLDAATPGRSERPKRRTVTSVPARGTRVIARPRG"
     gene            complement(4994..5824)
                     /locus_tag="VF752_02570"
     CDS             complement(4994..5824)
                     /locus_tag="VF752_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854457.1"
                     /translation="MKIRLIAACAVVAVAGVLAVAGTASAAPTWAPASSAAIHPGVQT
                     FTDGAQCTANFIYFDASNVYIGQAAHCSGTGGNTETNGCDSGTLPVGTKVDVTGASKP
                     GTMVYNSWATMQAKGESDPDTCQYNDLALIQLDPADVGKVNPSVPYWGGPVGLGGATN
                     QGDNVYSYGNSELRGGVTTLSPKKGISLGTDSGGWNHSVYTLTPGIPGDSGSGFLDKD
                     GKAFGVLSTVAIAPLAGSNGVGDLSHELGYLHANTSFTGVQLANGTEPFNGSKAPIGG
                     "
     gene            complement(5950..6708)
                     /locus_tag="VF752_02575"
     CDS             complement(5950..6708)
                     /locus_tag="VF752_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8854458.1"
                     /translation="MPILSTRELLRAAAQPSRYPAAVRRRWRMRQLRGDSVECPCCGG
                     RFRAFMTERGRPNAVCPRCGAQERHRALWLYLRDRTPLFDRPLRTLHFAPELIFRRRL
                     ESLPNLDYVSADLDSPEAMLHFDITAIPFDDDSFDAVLCSHVLEHVENDRAAMRELVR
                     VLRPGGFAIVLVPLDLSREETYEDPSVTAPEDRAREFWQHDHVRLYGRDFAERLEQSG
                     FSVTVDPYVRELPAHVRRRHALLDVEDIYVGTKD"
     gene            complement(6733..7650)
                     /gene="htpX"
                     /locus_tag="VF752_02580"
     CDS             complement(6733..7650)
                     /gene="htpX"
                     /locus_tag="VF752_02580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX8854459.1"
                     /translation="MQKTALPRDAGLQARMLVTLFLLGLVYTVLVGALVAAGAGAVTV
                     ALIAAGMFLFQLFASDKLALRMMGARRVSPAEAPELHAMIDRLCLQADLPKPHIAVAE
                     TSLPNAFAMGRSKKSATVCATTGIMDLLSQPELEGVLAHELAHIRHRDVVIMTVASFF
                     ATLAAYILQFGLFFGGDNDRNPGILGLIGISVAVYAVSFFLMQALSRYREFAADRGAA
                     IITGRPSALASALLKISGQMKRVPANDLRVAQELNAFFIVPARVKSSLAGLFSTHPPV
                     ERRIQALEQIEAQMSGIRPATSYRDLTAV"
     gene            complement(7650..7871)
                     /locus_tag="VF752_02585"
     CDS             complement(7650..7871)
                     /locus_tag="VF752_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854460.1"
                     /translation="MAGRELAAYIEAEFDRGRDLRSIIEDDFVRDRLAERPYLLEDLL
                     NDEELAQRARRSDGTLDFDQLETALGGRR"
     gene            8359..9000
                     /locus_tag="VF752_02590"
     CDS             8359..9000
                     /locus_tag="VF752_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854461.1"
                     /translation="MSTNRTRVVVADDHPLFREAIVRAVRDRPDLELVGEASSGREAL
                     ELVRELTPDVAVIDLKMPDLDGLEVARAVQRDGLPTRVAILTAFLDGTLVFQAIAAGA
                     SAYLSKDAERNAITDAIAAVARGETVLAPEAQAGIAGEVRVRGMEERPALTPREQEIL
                     EHVAAGRAAPEIAKLLYLSPATVKGHLQNLYEKLGVSDRAAAVAEGMRRGLLE"
     gene            8997..10280
                     /locus_tag="VF752_02595"
     CDS             8997..10280
                     /locus_tag="VF752_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8854462.1"
                     /translation="MSGAPTLARVPRSDAARVWAWIRVAAIPVIWAGERYVEHPAPRS
                     APFGVILACAGVYALLLLVAAYRPDMPRPGFVHQAVLDLAFISALTYTSGGPYSQLRY
                     AYFLLPIGAALRLRPSLTAVASVASVAAYLAVALLYPTDLVSIRRPGDFEFVQALYLA
                     WMGVAAVLLSLILTKRAEEVERLSATRGRLVAQALDAEDRERRRLAESLHDEAIQNLL
                     AARQELASESDGRDLSLVRLGLDRTIAQLRDAVFDLHPYVLDQAGLAAALEAVTARTA
                     ERAGLEWSVDVDAEATGVHDELFFSITRELVSNAARHSGGRRVSVRLRRRPAELVLEV
                     EDDGHGVDATRLRRAPLEGHIGLASATERVEALDGELEIATDPARGTLMRARIPLPPE
                     EEPHRIESAPLGPEARASGGGDGPVADRGQPHPAR"
     gene            10231..10458
                     /locus_tag="VF752_02600"
     CDS             10231..10458
                     /locus_tag="VF752_02600"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854463.1"
                     /translation="METDRWRIEGNLTLPAEGFRSRLSDFVNQRDREFFSIRDATLIA
                     LDHPNDVKQVPFVMVASRHIRLILPIGGEEA"
     gene            10455..11069
                     /locus_tag="VF752_02605"
     CDS             10455..11069
                     /locus_tag="VF752_02605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854464.1"
                     /translation="MTRARGVALGAGLALLGAAVWWRRNPSACPYGQRFWVEAPHPFI
                     TRARLAGALAPRSGERLLEVGPGTGYYTLAVADALGPEGRLDIVDVQQEMLDHTMRAA
                     SAGGLTNVTPHLSDARSLPFGDGEFAGAYLVTVLGEVPDQDVALRELRRVLEPGARLV
                     VGELFGDPHMVTEGSLRRRAEAAGFRYQRRFGPSFGYFARLLAV"
     gene            11200..13176
                     /locus_tag="VF752_02610"
     CDS             11200..13176
                     /locus_tag="VF752_02610"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX8854465.1"
                     /translation="MAFAIAPSPAGVQAQPGWKFGGQVNGNGPLPSDGHRPWAKPGDP
                     GIGRGQQPPAAQQPPQIGQPPSQVGQPPVQQPPQVAQPPVQKPPVEKPQVVTPAPAGP
                     GRDNNGSGNGNRIGNGSGSETGNGNGIGNGNRVTDGTGTDRKPENQIGSGNSGAGGQP
                     SLDISGPRQRPPGETPSTPADSAEQPASQPTAPTTPAPQATPPTANVTPSAKLGREQR
                     GNGLLAGGGATGRSPLTSLPAAPATLPSIIRPGVQVPSTAAAPATPAAAKRPAAAKKD
                     PTPRKRHGASVVERTVHDIVKVVPEPMKYLIATLAGLLVMAAFGWAFVTLRARSLRRQ
                     RAELLGEVGLLQAALLPEVPERLGALSMSVAYRPADGPGAGGDFYDAFQMEKGRVGIV
                     IGDVAGHGRDALARTALLRYTLRAYLETGLEPRKALQIAGRTLEEDMDAGFATAAVAV
                     YDPAAGTLTYSCAGHPPPILLGEGAHDPITVSSSPPLGAGEATGLRQTTAPLVPGATA
                     CFFTDGLAEARVDGDMLGRERLAETIASLGERPDATALLAELRSTGHVPDDMAACIIS
                     PLAARSTDRKPTERTEEIEVPHYGGRALFRFLMACGLDEQEAGEQTRLALAEGETNGG
                     AIVRVLVGDGEPRVTISPPVIELIEAYSRRRLAS"
     gene            13233..13406
                     /locus_tag="VF752_02615"
     CDS             13233..13406
                     /locus_tag="VF752_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854466.1"
                     /translation="MTTTPMHEAAELKMRELLEDNDLPQPDEIEHEETSIVLTWHESK
                     LVVMVDLTDAEDD"
     gene            complement(13384..14280)
                     /locus_tag="VF752_02620"
     CDS             complement(13384..14280)
                     /locus_tag="VF752_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8854467.1"
                     /translation="MATTSLQTSLWRLASDQHGVLSRDQLLANGLTAAAIKHRLRTGR
                     LFRVARGIYAVGRRDLTQEGCWMVAVLRCGEGAALSHASAAALWGIWRCDGVRPQVSV
                     AADVVRRPSGITVYRRTRFETTTERGIRVTTPICTLVDLAANFSPKVVEAAVNEADKL
                     DLVSPERLRAALEGLDHRPGAPALRKLLDRRTFTLTDSELERRFIPIAKRAGLPPPKT
                     QQLVNGYRVDFFWPDLGLVVETDGLRYHRTTAQQTKDHLRDQAHTAAGLVPLRFTHAQ
                     VKFEAKHVERTLRAVVSRLRRR"
     gene            complement(14362..14451)
                     /locus_tag="VF752_02625"
     tRNA            complement(14362..14451)
                     /locus_tag="VF752_02625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14415..14417),aa:Ser,seq:gga)
     gene            complement(14472..15047)
                     /locus_tag="VF752_02630"
     CDS             complement(14472..15047)
                     /locus_tag="VF752_02630"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX8854468.1"
                     /translation="MLEQLRPVLRAAAIAAFVTGLVLLLVNPPSLSGLGGLVAILAGA
                     LALVLITAWGTVALMGRDGMSEDEFERVVRRSEELARVPSLAREATEFELIVAEAIDA
                     LPPEFQAMLENTPVVVSQRGFEVHAYGRYYGDTVARGSYEDRIVIYQDTLERDFGWDP
                     EVLRAQVERTVRHELAHHLGWNEEGVRGLGL"
     gene            15113..15355
                     /locus_tag="VF752_02635"
     CDS             15113..15355
                     /locus_tag="VF752_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854469.1"
                     /translation="MTGLPLILILLILIGALVGAGSVIFTPVLAIPIGLFLIALVGIW
                     ELTKRRRTSTQRMEELREEADSDRVEFTERDRQTLA"
     gene            15374..16105
                     /locus_tag="VF752_02640"
     CDS             15374..16105
                     /locus_tag="VF752_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8854470.1"
                     /translation="MKPVHIGCSGWNYADWRDRLYPKGLPTGRWLERYAEVFETVEIN
                     SSFYRLVRPSAAENWVRQTPADFIFAAKASRYLTHIRRLRELDQGIKRFYEGIQPLVD
                     SGKLGPVVWQFPANFHRDDERLESALGKLPPGKHCFEFRHESWFVSEVYELLRRHGAA
                     LIIGDHPERPFQSYEFTTDWTFVRFHYGRRGRNGNYSKTELEEWADRLRKWRRRVEVF
                     AYFNNDWRGYAVENGKALERLLGLR"
     gene            complement(16115..16279)
                     /locus_tag="VF752_02645"
     CDS             complement(16115..16279)
                     /locus_tag="VF752_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854471.1"
                     /translation="MEHERKEESDQLPEEQPAEVVPDDVEDDAREEAEENPGVPGEEG
                     QATGEPGNAG"
     gene            complement(16296..16508)
                     /locus_tag="VF752_02650"
     CDS             complement(16296..16508)
                     /locus_tag="VF752_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854472.1"
                     /translation="MDSHESQTDKAERLPEDDDGFRLHVTPRGALVLQTENARRMMRR
                     LGRFAADADDVAADAVEPSGAAPAGP"
     gene            16705..17646
                     /locus_tag="VF752_02655"
     CDS             16705..17646
                     /locus_tag="VF752_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854473.1"
                     /translation="MKWTRTLGAVCVVVVAAAGSASAHRVPGAPRCPVFPANNQWNLR
                     VDKLPLIANSRAIVDSIGATAPVHADFGSGLYDGAPIGIPYKVVTRRQKAARVSFDYS
                     DESDHGRYPIPRNVPIEGGPKSDGDRHVIVVDRDRCRLYELFAAYPRSGGRSWHAGSG
                     AIWRLRSNKLRPSGWTSADAAGLPIFPGLARYDEVRGGSIDHALRITVPRSRRAFIYP
                     ARHFASDLTDADLPAMGQRLRLRASFPERGFPRQARVVIRALKIYGAIVADNGAPWFI
                     SGAPARGWNNDDLHSLGQVKGSDFEVVDTASLPRPGG"
     gene            complement(17655..18038)
                     /locus_tag="VF752_02660"
     CDS             complement(17655..18038)
                     /locus_tag="VF752_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854474.1"
                     /translation="MFDRLERQASEVGRVQARADRLESELERERTAHATVARRLELQN
                     AELEQLRGQARELADEREAREQLTNELADVRSVNDRLESHLGAAWQEVHAMRAELARP
                     WWRRRGRLVTSRAWAHSFEARAAQP"
BASE COUNT         2763 a         6571 c         6492 g         2778 t
ORIGIN      
        1 cgccgagcgc gtcgcgcgac cgaagccgcc gtcgatcgac cccggtgaga agccgcgccg
       61 gcgcagcagg aactgcaact cggacacatc ccacccccgg cgaccgcgcc ggagttggcg
      121 ccggccaagg tcgggacgcc cgcgccagcc gagcgccctg cgcgtgcgcg gccccgccac
      181 gccgtccacg gcgagatgcc gcctccgctg gagccggcgc acggcgcgcc gcgtggcagg
      241 accggcgatg ccgtcgatgg acccgcggta cagatgcagg ccagtcaagg ccacttggag
      301 cgcagcggtt cgcggagtgg cggccgacgc ctcggcgact ggcgcaagca gcagcgcagc
      361 gacgagcggc gccagaagag gcgacggctt cgaggagggc atcccccaaa agagatatcc
      421 cgttcgtacc ccccacgggc atcctgagaa ggctcttgca tgcaaggctc ccagcggtaa
      481 ccgtcgagaa aggacgcttc atggccgaag agctctccgg caaggtcgtc gccatcaccg
      541 gcgcgtcgtc aggcatcggc gaggcgaccg cgctcgcatg ttcgtgggcc ggcgcggcag
      601 tctcgctcgc agcccggcgg ctcgatcgca tcgaggagct ggccaggcgg atcaagtacg
      661 agggaggccg cgcgctcgcg atcgagaccg acgtcggcga cgaggaccag gccaacgcgt
      721 tcgtcgagcg cacaaacgag gagctgggcc gcctcgacgt gctcgtgaac aacgcgggcg
      781 tgatgctcct cggcccgatc gagaacgcgc ccaccgagga gtggcgccgg atgatccacg
      841 cgaacgtgtt cggcgtcatc tactgcactc acgccgcgct gccgatcatg cgcgcgcaga
      901 gcgccggcca catcgtgaac gtcagctcgg tggcagggcg cgtcgcacgc gcgggcgcgg
      961 gtgtctacaa cctcacgaag ttcggcgtgg gcgcgttctc ggaggcgctg cgccaggagg
     1021 tgacggcttc cggcatccgc gtcacgctcg tcgagccggg cgccgtcgcg accgagctcg
     1081 gctcgcacaa tcgaccggag atcctcgagg cgctgcgcag tgatctcggc agcgttactc
     1141 ggatggacgc cggcgacatc gcgaacgcga tcctctacac gatcacggcg ccggagaacg
     1201 tgagcgtcaa cgagatgctg atccgcccca ccgggcaggt caggtgaact tcggcagcac
     1261 ctctctggag taggtgctga agaagccctc aaggtcgggg ccaatctgct gcacgtacag
     1321 ctcgtccgcc ccggcgtcgg cgtactgctg aagcgcttgc agatgcttgt cgaggtcggg
     1381 cccgcagggc acggactccg cgaccatctc cttcgtgacg agctcggtcg cctgctcgaa
     1441 gtgggccggc gtgggaagca cctgcgccag ctcgcccggt agcacctcgt tcggccacag
     1501 ccggtgcacc tcgtcgatcg cgcgttgctc gtcctcgtcc cagcagacct tcgcgcccgc
     1561 ctgcaccggc ttgccctgac cgcccgcgga gtggaagtgc gagatcagat cgcggtcggg
     1621 catcgtgatc gcatagccgt cgcccactcg acccgccaac tccaccgacc tccggccgaa
     1681 gcccgagacg accaccggcg ggggctcggg cggcagcgtg tagatccggg cgttctccac
     1741 gcggtagtgc cttccgcggt ggctccgcgt tccgccctgc cacagcatcc gcatcacctc
     1801 gatcgcctcc tcgagcatct cgatgcgctc ctccgcctcg ggccagtggt cgccgaggat
     1861 gtgctcgttg aggttctccc cggtacccac gccgaggttg aagcggccgt cgagggccac
     1921 cgtggcggtg gcggcagcct gggcgatcac ggcggggtga atgcggatcg tcgggcacgt
     1981 gacggcggtc gtgacccgca ttcgcgaggt cgcctcggcg agctgcccga tcaccgtcca
     2041 gacgaacggg ctctgcccct gctcgtcgtt ccatgggtgg tagtggtccg agatccagag
     2101 atcctggaag cccgcctcct ccgctcggcg cgccttgtcc acgagctctc gcggtccgaa
     2161 ctgctcgcac gagaggaagt agccgatcct catttcgccc gtccccttcc tgtccgcctt
     2221 cgggtcggct ctcagttgct cgtttcgaga gaaggcaaac cgcgcggccg gtccggagct
     2281 cactattttt gtgtgcatga gcacgcagga cgacgacggc cacgcgatct cctaccaggc
     2341 gctgcgccgc ggcacccccg tgcgcgccgc ggacggcacc gaggtcggga ccgttcgcaa
     2401 ggtgctcgag aacacgcgcg agaacatctt cgacggcatc gtggtcgaga cccataaggg
     2461 ccggcgcttc gtcgacgcgc cggaggtcgc tcgcgtggca gagcgcgccg tgacgctcac
     2521 gatctccgca gaggacgtcg acgcgcttcc ggcgccgcgc tcggtgatga aggaccgctg
     2581 ggatcaggcg acgcttgtgc gccgtgcgag gcgcgcaggc cgcgacgctc gccgtcgctg
     2641 gcagaaccgc tgaggattca gtaagggtcg cggggcggct tcaggtcgcc ctgccagtcg
     2701 accggctcgc cgctgccgaa ctgctcttcg aggacgactt cctgctcgtg ctcgacctgc
     2761 ggctcgcgct tgctgccgcg gaatacgacc gccatgacga cggcgccgac gaggaccacg
     2821 gcgacgacga ggacgatcac accgagggtc gagatagcta ggaccatggc gcgagggtac
     2881 gctcgcggat cgtccggatc aactctcgag cgacgcggcg cgtgcctcgt ccggcgtgga
     2941 gaagacgcgc aggcggatcg cgacgtcgtc cttgagcgtg agcacgtggg cgatgtcctc
     3001 gctcacacgc actccggtga gccggccctc gccctcctgg cgcagcacga ccacgacgtt
     3061 gcggatcccg ccgtcgcgca ccgggccgag ctcgatcgcg gttggccgga tccggtagtt
     3121 ctcgaacgtg tcgtcggtct gctggaacgc cacgcgggcg ccgtcgtggc cgtggtagac
     3181 gcccgcctcg ggcgcctcgg cccggtcgcg cagctcgacg tcgtcggcga gcagcgcgag
     3241 cacagcgtcg acatccccgc gtgagagcgc ctcgtagccg ctgcgcagaa ccgctcgtag
     3301 ctcgtcgtcg tccatcgcca ccagtattcc gcgcgcgcgt gacatggcgc acacgtcgct
     3361 tgaccgcgca cagccgacaa gttgttatga gcacatgacg gacgtggccg tggaggcgcc
     3421 gccgagcgcg atcccgcctg gaccgcgcgc gctgccgctc gcgaacctct acgcctggct
     3481 gtcgcggccg atcgccatgc tcgagcgctg tcagcgccgc tacggcgacg tgttcgcgat
     3541 ggacttcggg gagaagcacg gacgcttcgt cttcgtcgcc gatcccggcc tgatccgcga
     3601 ggtgttcacc gagcagcgcg actcctttgt cgcgggcgct ccgaacagcg tgctgtcgcc
     3661 gatcgtcggc accggctcgg tgctcaccac cgacggcgct cgccatctgc gccagcgcaa
     3721 gctgctgctc ccgccgttcc acggcgagcg cctggaggcc taccgcagcc ggatcagggg
     3781 gcttgcggag caaacggtcg ccggctggcc cgagaaccgc acgttcgccg tcgccgactc
     3841 gttccagcgc atgacgctcg aggtgatcgt ggacgtggtg ttcggcgtct ccgacgagga
     3901 gcggcgccgg cgcctgctcg agatgctgcc gccgctcacg cgctcggcga ccacgatgat
     3961 cttcttcccc gtgctagagc gggacctcgg tccgctgagc gtcggcgggc gcgtgcgcag
     4021 gctgcgtgag gcggtcgacg agctgatcca cacggaaatc gccgcgcacc gcaccgccga
     4081 acgggagggc gagccgcgcg gcgacatcct cgcgatgctg atcgccgcac gggacgagga
     4141 gggcgaggcg ctcagcgacc aggagctgcg cgacgagctg atgacgctgc tgttcgccgg
     4201 acacgagacc accgcgaccg cgctcgcgtg gtcggtcgat ctgatcctcc agcaccctcg
     4261 ggtgcatggc cgcctcgtcg aggatctgcg cacgaacggt gacgagtacc tcgacgcggt
     4321 gatccaggag acgcatcgct tcaggccgac cgtcccgaat gttgtgcggg aagcgacgcg
     4381 cgacgtggag ctcggcgact ggctgatccc cgccggggac gtggtggcac ccgccatcca
     4441 cctcgtgcac cggcgccccg acctctaccc cgagccggac gtgttccggc ctgagcgctt
     4501 cctcgactcg cgcgcggaga cgtatggctg gctgcccttc ggtggcggca tccggcgctg
     4561 catcggagcg agcctcgccc agctcgagat gcgcgaaacg ctgcgcgtgg tgttcgagcg
     4621 cgttcagctc gacgccgcga cgccgggccg gtcggagcgg ccgaagcggc gcaccgtgac
     4681 ctcggtgccc gcgcgcggca cgcgggtgat cgctcgtccg cgtgggtagg cgtggaggct
     4741 taacgggagc tcgcccgccc gcgtaggcgc ggagcgctta cagcagcaat gagggtgggg
     4801 cccgagtcgt cgggttcgtc ttctgacgat tggtcgattt tgggtctctg agacccaaaa
     4861 tgcaccactc ttcaagaaaa gcggcgctcg atcagccgcc ggctgacggg gtgccctaag
     4921 cggagttcgt ggaccggcga agccggttca cccgcgtggg caccccgcag ccgggcggcg
     4981 taccgagaac gctttacccg ccgatcggcg ccttcgaccc gttgaacggc tcggtgccgt
     5041 tcgccaactg cacgcccgtg aacgaggtgt tcgcgtgcaa gtagccgagc tcgtgcgaga
     5101 ggtcgcccac gccgttcgag ccggctagcg gagcgatcgc caccgtgctc agcacaccga
     5161 acgccttgcc gtccttgtcg aggaacccgc tgccggagtc cccggggatg cccggcgtga
     5221 gggtgtagac cgagtggttc cagccgccgc tgtcggtgcc gaggctgatg cccttcttgg
     5281 ggctgagcgt cgtgacgccg ccgcggagct ccgagttccc gtagctgtag acgttgtcgc
     5341 cctggttggt ggcgccgccg aggcccaccg ggccgcccca gtacggcacg ctcgggttca
     5401 ccttgccgac gtcggccggg tcgagctgga tgagcgcgag gtcgttgtac tggcaggtgt
     5461 cggggtcgct ctcccccttc gcctgcatgg tcgcccaact gttgtagacc atcgtgccgg
     5521 gcttgctcgc gccggtcacg tcgaccttgg tgccgacggg cagcgtgccg gagtcacaac
     5581 cgttggtctc cgtgttgcca ccggtccccg agcagtgggc cgcctgtccg atgtatacgt
     5641 tggaagcgtc gaagtagatg aagttggcgg tgcactgcgc gccgtccgtg aacgtctgca
     5701 cacctgggtg aatcgcagcg ctggaagccg gcgcccacgt gggcgcagcg gacgctgtgc
     5761 cggcgaccgc gagcacgccg gcgacggcga ccacggcaca cgccgcgatc aagcggatct
     5821 tcacaacatc cccctgcggt tgattgcgtg attcggaaag gggcgccgcg tccgggactc
     5881 ccgcctccgt gcggcaactg ttcgtacccc gcgcgggaca gtacccaaac tcgggtaggc
     5941 gggcgccggt tagtccttcg tgccgacgta gatgtcctcc acgtcgagca gcgcgtggcg
     6001 ccgacgcacg tgcgccggca gttcccgcac gtagggatcg accgtcacgc tgaagcccga
     6061 ctgctccagg cgctccgcga agtcgcggcc gtacagacgc acgtggtcgt gctgccagaa
     6121 ctcgcgcgcg cgatcctcgg gcgcggtcac cgagggatcc tcatacgtct cctcgcggct
     6181 caggtcgagc ggcaccagca cgatcgcgaa acctcccggc cgcagcacgc gcacgagctc
     6241 gcgcatcgcc gcgcgatcgt tctcgacgtg ctcgagcacg tggctgcaga gcaccgcgtc
     6301 gaagctgtca tcgtcgaacg ggatcgccgt gatgtcgaag tggagcatcg cctcgggcga
     6361 gtcgaggtcc gcgctcacgt agtcgaggtt cgggagcgac tcgagcctgc ggcggaagat
     6421 cagctccggc gcgaagtgga gcgtgcgcag cgggcggtcg aacagcggcg tgcgatcacg
     6481 caggtagagc cagagcgcgc ggtggcgctc ctgcgcgccg cagcgcgggc acaccgcatt
     6541 cggcctgccg cgctcggtca tgaacgcgcg gaagcggccg ccgcagcagg ggcactcgac
     6601 cgagtcgccg cgtagttgcc gcatgcgcca gcgccgccgc acggcggctg ggtagcgcga
     6661 cggctgcgcg gccgcgcgca gcagctcgcg tgtcgagagg atcggcacgc gcgaaccgta
     6721 gtggcgcgcg ggtcagaccg ctgtcagatc gcgataggaa gtcgcgggcc ggatcccgct
     6781 catctgcgcc tcgatctgct cgagggcctg gatgcgacgc tctaccggcg ggtgcgtcga
     6841 gaagagcccg gcgagcgacg acttcacccg cgccggcacg atgaagaacg cgttcagctc
     6901 ctgcgcgacg cgtaggtcgt tcgcgggcac gcgcttcatc tgaccggaga tcttcagcag
     6961 cgctgaggcg agcgccgacg ggcggccggt gatgatcgcg gcgccgcgat cagccgcgaa
     7021 ctcgcggtag cgggacagcg cctgcatcag gaagaacgaa acggcgtaca cggcgaccga
     7081 gataccgatg agcccgagga tgcccgggtt gcggtcgttg tcgccgccga agaacaggcc
     7141 gaactgcagg atgtaggccg cgagcgtggc gaagaacgac gccacggtca tgatcaccac
     7201 gtcccgatgg cggatgtgcg cgagctcgtg cgcgaggacg ccctcgagct ccggctgcga
     7261 gagcaggtcc atgatccccg tcgttgcgca aacggtcgcc gacttcttcg agcgccccat
     7321 cgcgaacgcg ttcggcaggg acgtctcggc cacggcgatg tgcggcttgg gcaggtcggc
     7381 ctggaggcag agccggtcga tcatggcgtg gagctcgggc gcctccgcag gcgagacgcg
     7441 gcgggcgccc atcatccgca gggccagctt gtcggacgca aaaagctgga aaaggaacat
     7501 ccccgcggcg atgagcgcca ccgtcaccgc gccagcgccc gcggcgacca gtgcgccgac
     7561 gagcacggta tagacgagtc caaggaggaa cagcgtgacg agcatgcgcg cctgcagccc
     7621 ggcgtcacgc ggaagagcgg tcttctgcat cagcggcgcc ccccgagagc ggtttcgagc
     7681 tggtcgaagt cgagcgtgcc gtcgctgcgg cgcgcccgct gcgcgagctc ttcgtcgttc
     7741 agcaggtcct cgagcaggta cgggcgctcg gcgaggcgat cccggacaaa gtcgtcctcg
     7801 atgatcgagc ggagatcacg cccgcggtcg aactcggcct cgatgtaggc ggccagctca
     7861 cggcccgcca cgtaccggtc gagcagcgag aagcgcctgt cagaagtgga ggcccggcgc
     7921 gacgcccctc cgggcgccgc gccgggggaa tgcgccccgg aaccgggcag ggcaggcccg
     7981 tctccggggt tttgtgccgg cgccgtcccg acctccgagc tgcgccgtcc cgcgtgggca
     8041 gtgcgggtgg caggggcacg gaccgcaggg tccacgccat tgctcgatgg ccgttcgccg
     8101 cggccgggcg gactcacggc cggcccgggc gcctcgtgac tggcccgcca ctcgcgacag
     8161 acccaggacc gagccggagc ctgcccggcc gcggcggatg gacgtacaac cgacatggcc
     8221 gtgactctgg cgatggcggc atcccgcgca catcccgcgc gtggaccgct cgcgggcgtg
     8281 tttgcgtgat ccgatcggag gggttcgatc ggatggcacc gggcagtccc gcgcaatctg
     8341 gctggtagaa acggtgtcgt gtctacgaac cgcacgcgcg tggtagtcgc cgacgaccat
     8401 cctctcttcc gtgaggcgat cgtgcgcgct gtgcgcgacc ggcctgacct cgagctcgtc
     8461 ggcgaggcca gctcggggcg ggaagcgctc gagctcgtcc gcgagctcac gcccgacgtc
     8521 gccgtgatcg acctgaagat gcccgacctc gacggactgg aggttgcgcg cgcggttcag
     8581 cgcgacggcc tgcccacgcg ggtcgcgatt ctcacggcgt tcctcgacgg cacgctcgtc
     8641 ttccaggcga tcgcggccgg ggcgtcggca tacctcagca aggacgccga gcgcaacgcg
     8701 atcaccgacg cgatcgcggc ggtggcgcgc ggcgagacgg tgctcgcgcc cgaggcgcag
     8761 gccgggatcg ccggcgaggt acgcgtgcgc gggatggagg agcggccggc actcacgccg
     8821 cgcgaacagg agatcctcga gcacgtggcc gcgggccggg ccgcgcccga gattgcgaag
     8881 ctgctctatc tcagcccggc gaccgtgaag ggtcacctgc agaacctcta cgagaagctc
     8941 ggagtgtccg accgcgcggc ggccgtcgcc gagggcatgc gccgggggct gctcgagtga
     9001 gcggcgcgcc gacactcgcg cgcgtcccgc gcagcgatgc cgcacgggtg tgggcgtgga
     9061 tccgcgttgc ggccatcccg gtgatctggg cgggcgagcg ctacgtcgag caccccgcgc
     9121 cgcgcagtgc gccgttcggc gtgatcctcg cgtgcgcggg cgtgtacgcg ctgctgctac
     9181 tcgtcgccgc ctaccggccc gacatgccgc ggccgggctt cgtgcaccag gccgtgctcg
     9241 acctggcgtt catctcggcg ctcacctaca cgtcgggcgg gccgtactcg cagctgcgct
     9301 acgcctattt cctgctgcct atcggcgcgg cgctgcggct gcgcccgtcc ctcaccgcgg
     9361 tcgcgtccgt ggcgtccgtc gccgcctacc tggcggtggc gctcctgtat cccaccgatc
     9421 tcgtgagcat tcggcggccc ggcgacttcg aattcgtaca ggcgctgtac ctcgcctgga
     9481 tgggagtcgc ggcggtgctc ctttcgctga tcctcacgaa gcgcgccgag gaggtcgagc
     9541 gactgtccgc cacgcgcggg cggctcgtgg cgcaggcgct cgacgccgag gatcgcgagc
     9601 gccgacggct ggccgagtcg ctccacgacg aggcgatcca gaacctgctc gcggcgcgac
     9661 aggagctcgc gtccgagagc gacgggcgcg acctgtcgct cgtgcgcctc ggcctcgatc
     9721 gcacgatcgc gcagctccgt gatgcggtct tcgacctcca tccctacgtg ctcgaccagg
     9781 ccgggctggc ggcggcgctc gaggccgtga cagcccgcac ggccgagcgc gccggcctcg
     9841 aatggagcgt ggacgtggac gcggaagcga ccggcgtgca cgacgagctc ttcttctcca
     9901 tcacgcgcga gctcgtgtcg aacgcagcac gccactctgg cggccggcgc gtgagcgtga
     9961 gactgcggcg gcggcccgcc gagctcgtgc tcgaggtgga ggacgacggc cacggcgttg
    10021 acgcgacccg cctgcggcgg gcgccgctcg aggggcacat cggccttgcg tcggcgactg
    10081 agcgggtgga ggcgctcgac ggtgagctcg agatcgcgac ggaccctgcc cgcggcacgc
    10141 tcatgcgcgc gcggattccg ctgccgccgg aggaggagcc tcaccggata gagtccgcgc
    10201 cccttggacc tgaggctcga gcaagtggtg gtggagacgg accggtggcg gatcgagggc
    10261 aacctcaccc tgcccgctga aggcttccgc agccgcctgt ccgacttcgt gaaccagcgc
    10321 gaccgcgagt tcttctcgat tcgcgacgcc accctgatcg cactcgacca cccgaacgac
    10381 gtgaagcagg tgccgttcgt gatggtcgcg agccggcaca tccggctgat cctgccgatc
    10441 ggcggggaag aggcgtgacg cgcgcgcgag gcgtggcgct cggcgccggc ctcgcgctgc
    10501 tgggtgccgc cgtctggtgg cggcgcaacc cctcggcgtg cccgtacggg cagcgcttct
    10561 gggtcgaggc gccgcacccg ttcatcaccc gggcgcgcct ggcgggggcg ctcgccccgc
    10621 ggtccggtga gcggctgctc gaggtcgggc cgggcacggg ctactacacc ttggccgtgg
    10681 cggacgcgct cggcccggag ggtcgcctcg acatcgtgga cgtgcagcag gagatgctcg
    10741 accacacgat gcgcgcggcg agcgcgggcg ggttgacgaa cgtgacgccg catctctcgg
    10801 acgcacgctc gcttccgttc ggcgatggtg agttcgccgg cgcctatctc gtgacggtcc
    10861 taggcgaggt cccggaccag gacgtcgcgc tgcgtgagct gcggcgcgtg ctcgagccgg
    10921 gggcgcggct ggtggtcggg gagctgttcg gcgacccgca catggtcacc gagggatcgc
    10981 tcagacgcag ggcagaggcg gccggtttcc gctaccagcg gagattcgga ccgtcgttcg
    11041 gctacttcgc ccggctgctc gccgtctaag cgtttctgga cgcatctgga catttgttcg
    11101 atcttccctc gaagggctaa ttcgccgcgc ccagaaagcg atggcagaac catgccctcc
    11161 gcttggtccc gtcgtgcctc gatatgtgcc ctcgtggcga tggcgtttgc gatagcgccg
    11221 tcgcccgccg gcgtgcaggc gcagccaggc tggaagttcg gcggacaggt caacggcaac
    11281 ggcccgttgc cgagcgacgg ccatcggccg tgggccaagc ccggtgaccc cggcatcggc
    11341 cggggccagc agccgccggc tgcgcagcag ccgccgcaga tcgggcagcc gccttcgcag
    11401 gtcgggcagc cgcccgtgca gcagcctccg caggtcgcgc agccgcccgt gcagaagcct
    11461 ccggtggaga agcctcaggt cgtgacgccc gctccggccg ggccgggcag ggacaacaac
    11521 ggctcgggga acggcaaccg catcggtaac ggcagcggca gcgagacggg taacggcaac
    11581 gggatcggta acggcaaccg cgtcaccgac ggaactggca ccgaccgcaa gccggagaac
    11641 cagatcggtt cgggcaactc cggggctggc ggccagccgt ctctggacat ttccggcccg
    11701 cggcagcgtc cccccggcga gacgccttcg acccccgccg actcggcgga gcagccggcc
    11761 tcgcagccca cggctccgac aacgcccgct ccgcaggcca cacctccgac ggcgaacgtg
    11821 actccctccg cgaagctcgg gcgcgagcaa cgcggcaacg gcttgcttgc cggcggcggc
    11881 gcgacgggcc gctcgcctct gacgagcctt ccggccgctc cggccaccct ccccagcatc
    11941 atccgcccgg gcgtgcaggt gccgtcgacc gcggcggcac cggccacccc cgcggcggcg
    12001 aagcgtcccg ctgcggcaaa gaaggacccg acgccgcgca agcgtcacgg cgcctccgtg
    12061 gtcgagcgca ccgttcacga catcgtgaag gtcgtgcccg agccgatgaa gtacctgatc
    12121 gccacgctcg cggggctgct cgtgatggcg gcgttcggat gggcattcgt gacccttcgc
    12181 gcacgcagcc tgcgccgcca acgcgccgag ctgctgggcg aggtcggcct gctccaggcc
    12241 gcgctgctgc ccgaagtgcc cgagcgactc ggtgcgctct cgatgtcggt cgcgtaccga
    12301 ccggccgacg gaccgggagc gggtggcgac ttctacgacg ccttccagat ggagaagggc
    12361 cgcgtgggca tcgtgatcgg cgacgtcgcg ggacacgggc gtgacgcact cgctcgcacc
    12421 gcgctgctcc gttacacgct gcgcgcatac ctcgagacgg ggctcgagcc gcgcaaggcg
    12481 ctacagatcg ccgggcggac cctcgaggag gacatggacg cgggcttcgc caccgcggcc
    12541 gtcgcggtat acgaccccgc tgccggcacg cttacctact cctgcgcggg ccacccgcct
    12601 ccgatcctgc tcggcgaggg cgcccacgac ccgatcaccg tgtcgtcgtc gccgccgctc
    12661 ggcgcgggtg aggccaccgg cctccgccag acgaccgcgc cgctcgtgcc gggcgcgacc
    12721 gcatgcttct tcaccgacgg cctcgcggag gcgcgggtgg acggcgacat gctcggccgc
    12781 gagcgtctcg ccgagaccat cgccagcctc ggcgagcggc cggacgcaac cgctcttctg
    12841 gcggagctgc ggtccaccgg ccacgtgccg gacgacatgg cggcctgcat catctcgcca
    12901 ctcgcggcgc gctcgaccga ccgcaagccg acggagcgaa ccgaggagat cgaggtgccg
    12961 cactacggcg gccgcgcgct cttcaggttc ctgatggcgt gcgggctcga cgagcaggaa
    13021 gccggcgagc agacgcgcct ggcgctcgcc gagggcgaga cgaacggcgg cgcgatcgtg
    13081 cgtgtcctcg tcggcgacgg cgagccgcgg gtgacgatct ccccgcctgt gatcgagctg
    13141 atcgaggcgt attcgcgccg ccggctcgca agctgagcgc agtcgttact cgcgtaaccc
    13201 cacgttgacg atcgccgggc gatcgtccgg gcatgacgac tacgcccatg cacgaagcgg
    13261 ccgaactcaa gatgcgcgag ctgctcgaag acaacgatct gccccagccg gatgagatcg
    13321 agcacgagga gacgagcatc gtgctcacgt ggcacgagtc gaagctcgtg gtgatggtgg
    13381 atctcaccga cgccgaagac gactgaccac cgcgcgcagc gtccgctcaa cgtgcttggc
    13441 ctcgaacttc acctgcgcgt gggtgaagcg cagcgggacg agcccggcgg cggtgtgggc
    13501 ctgatcacgc agatggtcct tcgtctgctg cgccgtggtg cggtggtatc gcaggccgtc
    13561 cgtctccacc acaagcccaa gatccggcca gaagaagtcg acgcggtagc cgttgacgag
    13621 ctgctgcgtc ttcggcggcg gtagaccggc tcgtttggcg atagggatga accgccgctc
    13681 cagctctgag tcagtaaggg tgaaggtgcg gcggtcgagg agcttgcgca gtgccggcgc
    13741 cccgggtcgg tgatcgagcc cttcgagcgc ggcgcgcaat cgctcggggc tcacgaggtc
    13801 gagcttgtcc gcctcgttta cagcagcttc gacgaccttg ggcgagaagt tggccgctaa
    13861 gtcgacgagc gtgcagattg gcgtggtcac acgaatgccg cgctcggtcg tggtctcgaa
    13921 ccgcgtgcgc cggtagacgg tgatccccga cggccggcgc accacatccg ccgccaccga
    13981 tacctgcggc cgaacgccgt cgcagcgcca tatcccccac aacgcggcag cgctcgcgtg
    14041 gctcagcgct gcaccctcac cgcatctgag gaccgcaacc atccaacagc cctcttgcgt
    14101 gagatcacgg cgcccaacgg catagatgcc tcgcgccaca cggaacagcc ggcccgtccg
    14161 caaccggtgc ttgatcgccg cggcggtcag cccgttagcg agcagctgat ctcgtgacag
    14221 caccccgtgt tgatcgcttg cgagtcgcca gagggatgtt tgaagacttg tggtcgccat
    14281 cacgaccaaa agtcttcgaa cttcgcggtt cgccccggcg cggggtgcgg cggcggggcc
    14341 gagccgcgcg acggcgctca gcggagaggg agggattcga accctcgagg caggtcaacc
    14401 ccgcccacgc gatttccagt cgcgctcctt aagccactcg gacacctctc caggcgcccg
    14461 taagcgtagc ttcagagccc gaggccgcgc acgccctctt cgttccagcc gaggtggtgc
    14521 gcgagctcgt gccgcaccgt ccgctccacc tgcgcgcgca gcacctcggg gtcccagccg
    14581 aagtcccgct cgagcgtgtc ctgatagatc acgatgcggt cctcgtacga gccgcgggcc
    14641 acggtgtccc cgtaatagcg accgtacgcg tgcacctcaa acccgcgctg cgagacgacc
    14701 acgggcgtgt tctcgagcat cgcctggaac tcgggcggca gcgcgtcgat cgcctccgcc
    14761 acgatcagct cgaactcggt cgcctcgcgt gcaagcgacg gcacgcgcgc cagctcctca
    14821 ctgcgccgca ccacccgctc gaactcgtcc tcgctcattc cgtcgcggcc catcagcgcc
    14881 accgtgcccc aggcggtgat cagcaccaac gccagtgcgc cggcgaggat cgccaccaac
    14941 ccgccgagcc cggacagcga cggcgggttc acgagcaaga gcacgagccc ggtcacgaag
    15001 gcggcgatcg cggccgctcg caggacgggt ctcagctgct cgagcacacc acaagcgtag
    15061 gccgcgcgac cccggagttt ccgtgggttg cccggagggt aggtctctcg cgatgaccgg
    15121 actccccctc atcctcattc tcttgatcct gatcggggcc ctcgtgggtg ccggatcggt
    15181 gatcttcacg cctgtgctcg cgattccgat cgggctcttc ctgatcgccc tggtcggcat
    15241 atgggagctg acgaagcgcc gtcgcacgtc cacgcagcgg atggaagagc tgcgcgagga
    15301 ggctgactcc gaccgcgtgg agttcaccga gcgcgatagg cagacgctcg cctaagcgtc
    15361 acccggcggg gcagtgaagc ccgtccacat cggctgctcg ggttggaact acgccgactg
    15421 gcgtgaccgg ctctacccca agggactgcc aaccggccgc tggctcgaac gctacgccga
    15481 ggtgttcgag acggtcgaga tcaactcgag cttctatcgg ctggtacgcc cgagcgccgc
    15541 ggagaactgg gtgcgacaga cgcctgcgga cttcatcttc gcggcgaagg ccagtcgcta
    15601 cctgacgcac atcaggcgcc tgcgggaact ggatcagggc atcaagcgct tctacgaggg
    15661 catccagccg ctcgtcgatt cgggcaagct cgggccggtc gtatggcagt tcccggccaa
    15721 cttccaccgt gacgacgagc ggctcgaatc cgcgctcggc aagctgccgc cgggcaagca
    15781 ctgcttcgag ttccgccacg agagctggtt cgtgtcggag gtctacgagc tgctacgccg
    15841 ccacggcgcg gcgctgatca tcggcgatca ccccgagcgg ccgttccagt cgtacgaatt
    15901 caccaccgac tggacgttcg tgcgcttcca ctacggccgt cgcggccgca atggcaacta
    15961 ctcgaagacc gagctcgagg agtgggccga tcggctccgc aagtggcggc gtcgcgtgga
    16021 ggtgttcgcg tatttcaaca acgactggcg cggctacgcc gtcgagaacg gtaaggcgct
    16081 ggagcgactg ctcggactgc gctgagctgc cggctcagcc ggcgttgccc ggctcgcccg
    16141 tggcctggcc ttcttccccc ggcaccccgg ggttctcctc ggcctcttcg cgggcgtcgt
    16201 cctcgacatc gtcgggcacg acctcggcgg gctgctcctc cggaagctga tccgactcct
    16261 ctttgcgctc gtgctccatg gcgagcacgt tacgcctatg gccccgcagg cgccgcgccg
    16321 cttggctcca cggcgtccgc cgctacgtcg tctgcgtcgg ccgcgaatcg ccccagccgg
    16381 cgcatcatgc gacgtgcgtt ctcggtctgc agcacgagcg ctccgcgcgg agtgacgtgc
    16441 agacggaagc cgtcgtcgtc ctccggcagg cgctcggcct tgtcggtctg gctctcgtgt
    16501 gaatccatgc ggtcctcccc tgcgtcgcgc gcacgcgggc tgcgagccct tcggcgcctc
    16561 acctcgcctt accccattcg tgcataggaa aaaccgtgtg gcccgctatt tgcgggatgt
    16621 cgtcaccgaa atggcgcatg gactgcctga ccccgcctcg ggggccgcgc ggttccggcg
    16681 gtgcacgcgg ctacgctcgc gcgcgtgaaa tggacgcgga cccttggcgc ggtgtgtgtc
    16741 gtcgtggttg ccgccgccgg atcggcgtcc gcgcaccgag tgcccggcgc cccgcgctgc
    16801 ccggtcttcc cggcgaacaa ccagtggaac ctgcgcgtcg acaagctgcc tctgattgcc
    16861 aactcgcggg cgatcgtcga cagcatcggg gccacggccc ccgtgcacgc cgacttcggc
    16921 tcgggcctct acgacggcgc cccgatcggc attccataca aggtggtgac gaggcggcag
    16981 aaggcagcgc gcgtgagctt cgactactcc gacgagtcag accatgggcg ctacccgatc
    17041 ccgcgcaacg tgcccatcga gggcgggcct aagtcggatg gcgaccggca cgtgatcgtg
    17101 gtcgaccgcg accgctgtcg gctgtacgag ctattcgccg cctatccgcg aagcggtggc
    17161 cgctcctggc acgccggctc aggcgcgatt tggcgcctgc gctcgaacaa gctgcgtccg
    17221 agcggctgga cctccgccga cgcagccggc ctgccgatct tccccgggct tgcccgctac
    17281 gacgaggtgc gcggtggctc gatcgaccac gcgctgcgga tcaccgtgcc gcgctcgcgg
    17341 cgcgcgttca tttaccccgc gcgccacttc gcctcggacc tgaccgacgc cgacctgccg
    17401 gcgatgggcc agcgcctgcg cctgcgcgcg agcttcccgg agcgtggctt cccgcgccag
    17461 gcacgcgtgg tgattcgcgc gctgaagatc tacggcgcga tcgtcgccga caacggcgca
    17521 ccgtggttca tcagcggcgc gcccgcacgt ggctggaaca acgacgacct ccactcgctc
    17581 gggcaggtga agggatcgga cttcgaggtg gtcgacaccg cctcgctccc gcgcccgggc
    17641 ggctagcgcg ctgactacgg ctgagcggcg cgcgcctcga agctgtgcgc ccacgcgcgc
    17701 gaagtcacga gcctgccgcg gcggcgccac caggggcgcg ccagctcggc gcgcattgcg
    17761 tgcacttcct gccacgcggc gccgagatgc gactcgagcc ggtcgttgac actgcgcacg
    17821 tcggcgagct cgttggtcag ctgctctcgc gcctcgcgct cgtcggcgag ctcccgggcc
    17881 tgaccgcgca actgctcgag ctccgcgttc tgaagctcca ggcggcgcgc gacggtcgcg
    17941 tgggctgtgc gctcgcgctc gagctcgctc tcgagccgat cggcccgcgc ctgcacgcgc
    18001 ccgacctcgg aggcctggcg ctcgaggcgg tcgaacatgt cggagatcag cgcgagctga
    18061 gcctcgacgg tcagcggttc cctctcgatc gcctcccgtg cgactcgctg cgcctcccct
    18121 gtcctgagtg cctgtacctg agaaaaggcg atacgaagag gctaccgaga tttgctcaaa
    18181 attgcgcgag atgatgaaaa aaatcagtgc aagatctgcg ccatgaccga gccggccgcc
    18241 accagagggg gcgagcgggt aacccttcgc gggggcgagc aggtggtgat caggccgatc
    18301 cggccggcgt cggccgaggc cggcgcgccg ctcgcgacag cactccgcca cgccccccgc
    18361 ggggcgcacc acggggcggc tgcgcggcgg tccgacccgc gatcgatgaa caggtttggc
    18421 ccgcgaccgc ccttggttac cttctccgtg tgtcctcgcg ccggacactg attgcagtct
    18481 ctggcctcgt cggcgcggcg ctcgtggtca tcgtgctcgc catcgctggc gtgttcagcg
    18541 gcagcgacga caactcccgc aacgtggtga tcggcggctc cgcgttcgcg cagggcggtg
    18601 cgac
//
LOCUS       DASVXS010000050        17918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_643119, whole genome shotgun sequence.
ACCESSION   DASVXS010000050 DASVXS010000000
VERSION     DASVXS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17918
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643119"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VF752_02665"
     CDS             <1..826
                     /locus_tag="VF752_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX8854475.1"
                     /translation="DFALWKAQKEGEDTAWDAPWGAGRPGWHIECSAMAEVILGLDFD
                     VHGGGSDLIFPHHENEIAQTEAGRGHQLARLWMHNGMLQMADEKMAKSVGNVTSLNDA
                     LALAGRDALLMYFVSGHYRQPIAFSEEALREAARAVDRVRELARRLDRDAPAPEALEP
                     YADRFFDALADDFNTPAARAALFDWVSEANRRLDAGERLGVGELEDMLRVFGLENLLD
                     VEEEPADPEAEQLLREREEARQARDFSTADAKRDELAARGWEVRDTPEGPRLVRAG"
     gene            833..1528
                     /gene="rlmB"
                     /locus_tag="VF752_02670"
     CDS             833..1528
                     /gene="rlmB"
                     /locus_tag="VF752_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX8854476.1"
                     /translation="MIVYGRNPVREALRGRRRVERVWATPKAAREDWLREAPGLSQVD
                     AAEIEVLAGSPDHQGVCAEVDAYPYADADSLLEADEALVVALDEVTDPRNLGAIVRVA
                     EAAGATGVVIPERRSAEVTPVVAKASAGAVEHLPVARVCNLADWLGEAKDAGAWVYGA
                     SADGIPYERPDYRGKVVLVLGSEGRGLRPRVAAMCDELVALPLRGKVDSLNVSAAAAA
                     LLYGVLQFRSQRG"
     gene            1715..2356
                     /gene="sigH"
                     /locus_tag="VF752_02675"
     CDS             1715..2356
                     /gene="sigH"
                     /locus_tag="VF752_02675"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX8854477.1"
                     /translation="MSASRSNAQPQLDDHYLIALAKQGKKDAYDQIVRRYYGFVRLKA
                     SSYFLVGGDSDDLIQEGLVGLYKAVRDYRYDRESSFRNFAELCITRQIITAVKTATRN
                     KHTPLNQYVSFSQTPASAGDGEPTLDEVLPGPTAHDPVNQVISTEELHSLVACLSSVL
                     SELESSVLSLYLDGHSYEAIGERLVCDTKTVDNALQRVKRKVGAHLASRNVML"
     gene            2445..3269
                     /locus_tag="VF752_02680"
     CDS             2445..3269
                     /locus_tag="VF752_02680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8854478.1"
                     /translation="MPALVRPLLVALLLAMLGFMAAASASRTHHVRAEKTAAKPAKSS
                     GFADPAPAPVTQPAADVQAARPARPRSTPQGFRVVRVRQGRSILIHNSVNGRPVGRLA
                     SSTEFGSPEVLSVAKRRGRWLGVTTPAIANGKLAWIRGESKALERDTTRWSLRADLSR
                     RRLELRNGGKLVRAMTVAIGRPGSTTPTGRFQVTDKLSGANYGAYYGCCILALSGHQP
                     HTPPGWQGGNRLAIHGTNVAATIGKAASAGCLRAADRDLRVLMRRVPLGTPVFIRS"
     gene            3376..3852
                     /locus_tag="VF752_02685"
     CDS             3376..3852
                     /locus_tag="VF752_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854479.1"
                     /translation="MDESATLELLGEAFERGEPVEVPDELVSRLPGEVEDEDPDFVSG
                     VTITEEDADDAIRRFAAAASGPELKAALEGLLQQSYEQKLAFLHADEWVFPPDHVTKP
                     PSKCVRKCERICQPVMQKVCRKVCEPRVPGGPIVCRPVCELVAKTVCKQVCVVICN"
     gene            3864..4706
                     /locus_tag="VF752_02690"
     CDS             3864..4706
                     /locus_tag="VF752_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEX8854480.1"
                     /translation="MSSLEEALAQLWAGEQAVYYDAEADDRDRASYYADVSNGLDPAQ
                     LFQALSRLVSDTHRRQPEYRPHVEVYPWVDLQPDRTLRSIYTDEVYDPETLIREDAET
                     EERRRDERARLITEGLTLRDLEAELETTLPYNCEHVYCQSWFDHAEPMRGDVHHLFAC
                     ESKCNSFRGNFPYDDFPDYPEVVRDPCGRRERSGFEPAHGKAKAARAALYFLIRYPEV
                     SAASPRYDAEHVTMLQGWHAASGPEEHERHRNAAIFARQGNRNPLIDFPEWSERIDFG
                     ARPG"
     gene            4752..5402
                     /locus_tag="VF752_02695"
     CDS             4752..5402
                     /locus_tag="VF752_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8854481.1"
                     /translation="MEVLVAGGHGQVARRLLRALAEQGHRGRGLIRNPDQAADLEADG
                     AEAVVLDLEQDAIEDDLSDVVSSADAVVFAAGAGPGSGAERKQTMDFGGAVKLMDAAK
                     AAGVRRYVIVSSMGADDPESASGPMGPYLEAKAAADRALEASGLDYTIVRPGGLTNDP
                     GTGRIEAATKLGRRGQVPRDDVAQTLLATLFAPTTIGKTFEVLSGDTPIQEAVRSV"
     gene            5493..5963
                     /locus_tag="VF752_02700"
     CDS             5493..5963
                     /locus_tag="VF752_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854482.1"
                     /translation="MERSRSHLAGAIYGQILATSLVATLSEDESATAGDIFFWVGVTM
                     LVFWAAHVYANWLARQVTTGGGLSELGSVAAEQWPMAQAAAPALGLLLLGWAGVYSRD
                     TAVDLAIAAGIISLTAWGVVIARRADANQLETLATAVISAGFGLLMVGLKILVH"
     gene            5967..6566
                     /locus_tag="VF752_02705"
     CDS             5967..6566
                     /locus_tag="VF752_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX8854483.1"
                     /translation="MPKWMGVDVGGKRKQFDVALIDESRLLELLDRQRVEDVVAWVDS
                     VRPTIVAIDSPRSCAPPGHGVRPDERALRAAVCGIRWTPSRDRLDGSDYYEWIREGLQ
                     LYEALEGLAAEVIECFPTAAWTRWSGERGGRARSSWTREALARLELADVPLRTNQDQR
                     DAIAAAVTAREYAHGRCECFGEIVVPMRRQPSRRGDSNP"
     gene            complement(6540..6612)
                     /locus_tag="VF752_02710"
     tRNA            complement(6540..6612)
                     /locus_tag="VF752_02710"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6577..6579),aa:Thr,seq:tgt)
     gene            complement(7344..7820)
                     /locus_tag="VF752_02715"
     CDS             complement(7344..7820)
                     /locus_tag="VF752_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854484.1"
                     /translation="MSSSRRLTFAGLLVAVIAAFACQAGAAGGQSGGSPAVATAGQAR
                     VLLLYNCQRGRYKPRKVIVTCADANFRVRGIVWSRWTQTEARGRGTALVNDCNPNCAG
                     GTFRTYPVRLHAFLPRMTGGCVPHSLFTRLAWRFPESKPEGPRKGTAKLVCPPSGP"
     gene            complement(8578..9717)
                     /locus_tag="VF752_02720"
     CDS             complement(8578..9717)
                     /locus_tag="VF752_02720"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8854485.1"
                     /translation="MRSTRAGLAAALVAVACAFVAPAAIAQGVTKAGLQRQLEALVAA
                     DGGPPGAIVTLRRGSRMTTLRAGVADVETGRKPRPTDHMRIASVAKAFSGAVALRLVA
                     SGKLELSETIGERLPSLPSAWSAVTLQQLLNHTSGLPDYTQSDGFRMQAESDPQGHVS
                     PATILSWVEREPLVFTPGSRYEYSNTDNIVVGLMAEQAGGRPYGRLLREIVFNPLELT
                     RTSFPSNPPLPQPFIHGYLVEPGSPPEDVSTLLSPSGAWSSGAIVSTPRDLGTFIRGY
                     LGARLFPASLQRRQLRFVAGGESSPPGPGRNSAGLAVFRYRTRCGNVYGHTGNFPGYV
                     QFAAATRDGSRAVTTSLNIPAPSGALLARLRAMQQTAVCLLLLPK"
     gene            10045..11184
                     /locus_tag="VF752_02725"
     CDS             10045..11184
                     /locus_tag="VF752_02725"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX8854486.1"
                     /translation="MPCFRVFLVASAAVLGSLLFAAGSAAAASGPKVTTVVSGLDNPR
                     DLAFGPRGRLYVAEAGHGGSECVSAGEEGTACFGFSSGISRVNIQAGRSHRVVSGLAS
                     TAEETGSSATGVDGISFLGNGSMFGIMAGSRDAIPPGLSAATTTKLQQQAGRLIRVTP
                     GRGVRFGADVGHEDFTWSAAHGDLVPGQFPDANPYGVLALPGATWVVDAGSNTIDRID
                     ARGKVHVVAFIPNPPSSDAVPTCIDRSRHGVLYVGELTGGGNQPGASVVWRFTPRGHK
                     LTQWATGLTAVTGCGFAHNGKFYATEFSTAGLDNAAPGTGAVVRVAPHSTSPRTVVSN
                     LNFPGGFAAGGDGSLYVSNWSISPAGGGEMPSGEVLRIALRAKHR"
     gene            11209..11331
                     /locus_tag="VF752_02730"
     CDS             11209..11331
                     /locus_tag="VF752_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854487.1"
                     /translation="MVVNETDLPDQVVREAVDTYIEEHASMPGVNQATRNCGAM"
     gene            11579..12256
                     /locus_tag="VF752_02735"
     CDS             11579..12256
                     /locus_tag="VF752_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8854488.1"
                     /translation="MARTLEVERVYRHFCMEARALEVVGERWSLLIVRDLLPGPRRFT
                     DLIRSLNPITPTRLTDRPRRLEAAGVVEREPAERGNEVWYRLTDAGRDLGPAIDELTF
                     WGIQHATERPRADEPVHGEAAMIGTKVWLNRYSSAPPGGLVWVWRFPGEDSYSLRATD
                     GAWDLTRGEQEGAAVTVDATTADWAVFLTSPREKRRLPTKDVRLEGSKAEVTRFVKAF
                     AAKPAAR"
     gene            complement(12222..13790)
                     /locus_tag="VF752_02740"
     CDS             complement(12222..13790)
                     /locus_tag="VF752_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854489.1"
                     /translation="MNQEPEKPMVLVALLLAAFMINLDTTIVNVALPTLVRELHASTV
                     QLEWIVDAYNLMFAAFVLAAGNLGDRLGRKGVLLAGLLVFGAASFAGGLGNSPGELIA
                     ARAVMGLGAALIFPATLSLLTNVFTERRERARAIGLWGASTGVGIAVGPIIGGWLLER
                     FSWSSVFFALVPFAAVAAVLVAFAVPTSRDPEAPPADRPGLVLSTAAMAALIYTIIEA
                     PNRGWAAGLSLAGFALAGVLLATFIAWERRVREPLLDIRLFRNLRFSAASGAVTITFF
                     SLMGFIFVITLYFQFLKGYGPLSTGVRLLPVAVLTGITSVLGTRLAVRRGTKLVVASG
                     MVCLAIGLAWASTSSTSTSYLTIAASMVFIGSGIGLTSAPATESIMGAVSAAKAGVGS
                     AVNDATRITGATLGVAVIGSIYASIYASRLADSLPAAVPGSIADSAQRSVGTAFGIAA
                     RLDATGRPRLAQGLHEAASNAFFDGFQIAVLVAVAITLVGAVAAAIVIPSQPPEVACE
                     PIEISGPQVSPQRP"
     gene            complement(13812..14375)
                     /locus_tag="VF752_02745"
     CDS             complement(13812..14375)
                     /locus_tag="VF752_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8854490.1"
                     /translation="MRKLNVSTLVTLDGVIQDPGGFQELAEGGWGSRYFDDEAQSYAL
                     EHLRESDAFLCGRVTYEVFKEFWPQVKEGEYAQTLNQMPKFVASSTLKEPLEWNATLL
                     QGDVGDAVAELKRQEGRDIVTYGSAGLMATLMGRNLIDEYRIWVHPLVIGTGTRLFAE
                     GVPTSSLELAESRTLGTGVVILVYRPL"
     gene            14550..16169
                     /locus_tag="VF752_02750"
     CDS             14550..16169
                     /locus_tag="VF752_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854491.1"
                     /translation="MQQRGVLEIAEAERRRWLALYVLCVGMLMIVLDATIVNVALPSI
                     REDLGFGQNDLAWVLNAYLVAFGGLLLLSGRIGDLIGQRRVFLVGLCVFSGASLLCAL
                     SESQGLLIGARFVQGVGGALTSAVILGMIVTMFPEPRAQARAIGIYTFVAVAGGSVGL
                     LLGGVLTQTINWHWIFFVNIPIGVATALLAIRLVPDPDGIGLAAGADLPGAALLTGGL
                     MLGVYTILGVAEEGWGSIQTLALGAVAIGLLAVFLARQARVANPLIPLRLFRSRNVSG
                     ANVVQALLVVGMFGMFFLGALYMQQILGYDPLQVGLAYLPLTIAMGTMSFRFTGQLNL
                     RFGPEATLAPAMAFVLAGLLLLARTPIDATYVSDLLPPMVLIGLGAGLGFPSLMTLAM
                     SGATESDSGLASGLVNTSVQVGGAIGLAVLATLATERTDNLVADGEPAAAALNSGYHV
                     AYLTGAGLVLVAIAVAVSFLRARVAEGSPEPEPVPEVPGEPVPDAATEPGTPSLRVKP
                     LPAYAQAAVAGCQETGMRVVIGCGGCAGSAQ"
     gene            16185..>17918
                     /locus_tag="VF752_02755"
     CDS             16185..>17918
                     /locus_tag="VF752_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8854492.1"
                     /translation="MASDYAPTPVEDFLDPDDPTLFPQLTPEQIEYLAEIGTQLTFAR
                     GDVVFEHGQRETPLYVVQSGAIDIIDHAPEGDRYFTQCRAGTFAADISMFTGEPTLAA
                     GYAAEPTSLVALPPEEVRRVVATTAELGDLLLRTMVTRREWLQGRGLGQQRLIGSRWS
                     GESFAVRELLERNLVPFTWHDLASDADSRILIEGLGIGEAECPVLVRSDSVIRRATVT
                     SVADELGLRAQVDGQSFDVVVLGGGPAGLATAVYASSEGLSTLVVERFAPGGQAGTSA
                     RIENFLGFPTGLTGAELARRATLQARKFGAVLSSVHEACEISEPQDGFREIGLADGQR
                     VRGRCVVLAPGADYRRLPADNADRFEGLGLYYAATHIEAQQCSEEDVVVVGGANSAGQ
                     AVVKLADHAREVHVVARRPLEAAMSRYLVNQIEAKSNVRVWSGCEVRSLEGDEKLESV
                     VISGAGPDRRVETSALFAMIGASPRSQGLVQFVGQDDKGFVVTGEEARRHPDFPRHWN
                     QEGRGPLLLECTRPGVFAVGDVRTGSTKRVAAAVGDGALVVRSLHTALASGQAQVNDD
                     RKLRAPAPQTTP"
BASE COUNT         2778 a         5976 c         6350 g         2814 t
ORIGIN      
        1 ggacttcgcg ctctggaagg cgcagaaaga gggcgaggac accgcctggg acgctccctg
       61 gggggccggg cggccgggct ggcacatcga gtgctcggcg atggccgagg tgatcctcgg
      121 cctcgacttc gacgtccacg gcggcggctc ggacctcatc ttcccgcacc acgaaaacga
      181 gatcgcccag accgaggccg gccgcgggca ccagctcgcg cgcctgtgga tgcacaacgg
      241 catgctgcag atggccgacg agaagatggc caagtcggtc gggaacgtga cgtcgctgaa
      301 cgacgcgctc gcgctggccg gccgcgacgc gctgctcatg tacttcgtga gcggccacta
      361 caggcagccg atcgcgttca gcgaggaggc gctcagggaa gcggcgcgcg cggtcgatcg
      421 cgtgcgcgag cttgcgcgcc gcctcgatcg cgacgctccg gcgccggagg cgctcgagcc
      481 ctacgccgat cgcttcttcg atgcgctggc cgacgacttc aacacccccg ccgctcgcgc
      541 ggccctgttc gactgggtct ccgaggcgaa ccgccggctc gacgccggcg agcggctggg
      601 cgtcggcgag ctcgaggaca tgctgcgcgt gttcggcctc gaaaacctgc tcgacgtgga
      661 ggaagagccc gcggacccgg aggccgagca gctgctgcgc gagcgcgagg aggcgcgcca
      721 agcgcgcgac ttcagcacgg ccgatgccaa gcgcgacgag ctcgccgccc gcggctggga
      781 ggtgcgggac acgcccgaag ggccgcgcct cgtacgcgcg ggctgaatcg cgatgatcgt
      841 ctacggccgc aacccggtgc gcgaggcgct gcggggcagg cggcgcgttg agcgggtgtg
      901 ggccaccccg aaggccgcga gggaggactg gctgcgtgag gcgccggggc tgtcgcaggt
      961 ggatgccgcc gagatcgagg tgctggcggg gtcacccgac caccagggtg tgtgcgccga
     1021 ggtggacgcg tacccctacg cggacgccga ctccctgctg gaggcggacg aggcgctcgt
     1081 ggtggccctc gacgaggtga cggatccgcg caacctcggc gcgatcgtgc gcgtggccga
     1141 ggccgccggg gcgaccggcg tcgtgatccc ggagcggcgg agcgcagagg taacgccggt
     1201 cgtggccaag gcgtcagccg gcgccgtcga gcacctgccg gtcgcgcgcg tgtgcaacct
     1261 cgcggactgg ctcggagagg ccaaggacgc gggcgcgtgg gtgtacgggg cttctgccga
     1321 cgggatcccg tacgagcgcc cggactaccg cgggaaggtg gttctcgtgc tggggtccga
     1381 gggccgcggg ctgcgcccgc gcgtggccgc catgtgcgac gagctggtgg ctctgcccct
     1441 ccgcgggaag gtcgattcgc tcaacgtttc ggctgccgcg gccgccttgc tctacggcgt
     1501 cttgcaattt cgctctcagc gcggttgaca aggctccata acttcgacca tactgcgcgg
     1561 cgacagatgg tctgaacctt aggtagagcc ttcgagttca gctaaatctg gcgccagggg
     1621 aggtagtgcg ccacggttcg aaaagggccg tgcctgctgg gcatgtcccg aaaggggaac
     1681 gttcgtggct atcagtacct cgccggcacg gccgttgagc gcatcaaggt ctaacgctca
     1741 acctcagctt gatgatcact acctgatcgc gctcgccaag caggggaaga aggacgcgta
     1801 cgaccagatc gtgcgccgtt actacggctt cgtccgcctg aaggcctcct cctacttcct
     1861 cgtcggcggg gattccgacg acctgattca ggagggtctt gttgggctct acaaagcggt
     1921 tcgcgactat cgctacgacc gcgagtcgag ctttcgaaac ttcgcggagc tctgcatcac
     1981 gcgccagatc atcaccgcgg tgaagacggc gacacgcaac aagcacacgc cgctcaacca
     2041 gtacgtgtcg ttctcgcaga cgccggcgtc ggccggcgac ggcgagccca cgctcgacga
     2101 ggtgcttccc ggcccaaccg cgcacgaccc ggtgaaccag gtgatctcca ccgaggagct
     2161 gcacagcctc gttgcctgcc tctcgagcgt gctttcagag ctcgagagct ctgtgctctc
     2221 gctgtacctg gacggccatt cgtacgaggc gatcggcgag cggctggtgt gtgacacgaa
     2281 gacggtcgac aacgccttgc agcgcgtgaa gcgcaaggtg ggagcgcacc tcgcttcgcg
     2341 caacgtgatg ctgtagccgc tgggcgaaac gcacctggac ggacgtccag ggcgcgcctg
     2401 cctgactcgc gagccgccgg catccggccg accccttggg tcggatgccc gcactcgtca
     2461 gaccgcttct cgtcgcgctc ctgctggcca tgctgggctt catggcggct gcctctgcgt
     2521 cccgcacgca ccacgtccgc gccgagaaga cggcggcgaa acccgcgaag tcctcgggat
     2581 tcgccgaccc ggctcccgct cccgtgaccc agccggcagc cgacgtgcag gccgcgcgcc
     2641 cggcgcgccc ccggtcgact ccccaaggct tccgagtggt gcgggtgcgc cagggccgct
     2701 cgatcctcat ccacaacagc gtcaacgggc gccccgtcgg ccgccttgcg tcgtccaccg
     2761 agttcggctc gcccgaggtc ttgtcggtcg caaagcggcg cgggcgctgg ctgggcgtca
     2821 ccacgcccgc gatcgcgaac gggaagctcg cctggatccg gggcgagagc aaggcgctcg
     2881 agcgagacac cacgcgctgg tcgctccggg cggacctgtc gcgccggcgg ctcgagctcc
     2941 gcaatggcgg caagctcgtg cgcgcaatga cggtggcgat cgggcgcccc ggctcgacca
     3001 cgccgaccgg ccgcttccag gtcacagaca agctctccgg ggcgaactac ggcgcctact
     3061 acggctgctg catcctcgcg ctgtccggcc accagccgca cacgccgccc ggctggcaag
     3121 gcggcaaccg gctcgcgatc cacggcacta acgtcgccgc gacgatcggc aaggccgcat
     3181 cggcgggctg cctgcgcgcc gcggaccgcg acctacgcgt gctgatgcgc cgcgtgccgc
     3241 tcggcacgcc tgtgttcatc cgcagctaga cgagttgcgc gggtcccgac ggtccgtgta
     3301 ggctgcgcgc tcaagtttgc ctcagcgttc tctccccctt gaacgtcagc cggatccata
     3361 cgacgagggg gagcgatgga cgagagtgcg acgttggagc tgctcggcga ggcattcgag
     3421 cgaggggagc cggtcgaggt cccggatgag ctcgtgtccc ggcttcctgg tgaggtcgag
     3481 gacgaagatc cggacttcgt cagcggcgtc accatcaccg aagaggacgc ggacgacgcg
     3541 atccggcggt ttgccgcagc ggcgtctggg ccggagctga aggcggcgct ggagggcctg
     3601 ctccagcagt cctacgagca aaagctggcc ttccttcacg cggacgaatg ggtcttccct
     3661 cccgaccacg tgacgaagcc tccctcgaaa tgcgtccgga aatgcgagcg gatctgccag
     3721 cccgtaatgc agaaggtctg ccggaaggtc tgcgagccgc gggtaccagg cggcccgatc
     3781 gtctgccgac ccgtgtgtga gctggtggcg aagacggtct gcaagcaggt ctgcgtcgtg
     3841 atctgcaact agcggggccc gggatgtcgt cgctagagga agcgctcgcc cagctatggg
     3901 cgggcgagca ggccgtgtac tacgacgccg aggccgacga tcgcgatcgt gcctcctact
     3961 acgcagatgt gtcgaatggg ctcgaccccg ctcagctctt ccaagcactg tcacggctgg
     4021 tgtcggacac acaccgcagg cagccggagt accggccgca tgtcgaggtc tacccctggg
     4081 tggatctgca gccggaccga acactgagaa gcatctacac cgacgaggtc tacgaccccg
     4141 aaacactcat tcgcgaggat gcggagaccg aagaacggcg ccgcgacgag agagctcgct
     4201 tgatcaccga ggggctgacg ctacgcgatc tcgaggccga gctcgagacg acgctgccgt
     4261 acaactgcga gcacgtgtac tgccagtcgt ggttcgacca cgccgagccg atgcgcggag
     4321 acgtccacca tctgttcgcc tgcgagtcga agtgcaacag ctttcgcggc aacttccctt
     4381 acgacgactt ccctgactat cccgaggtcg tccgcgatcc ctgcgggcgg cgagaacggt
     4441 cggggttcga accggcgcac ggaaaggcga aggcggcgcg ggcagcgctg tacttcctga
     4501 tccgataccc ggaggtgagc gcggcgagcc cgcgctacga cgccgagcac gtgacgatgc
     4561 tgcaggggtg gcacgccgca tcaggcccgg aagagcacga gcgacaccgg aacgcggcca
     4621 tcttcgcccg acagggaaac cgcaatccgc tgatcgactt tccggagtgg tccgagcgga
     4681 tcgatttcgg cgcccgtccg ggataggcca cgcccgtttg tccggcggcg agccgggata
     4741 ctgagcggcg agtggaggta ctcgtcgcag gagggcacgg ccaggtggcg cgaaggcttc
     4801 ttcgcgcgct cgccgagcag ggccatcgcg ggcgtggcct gatccgcaac cccgaccagg
     4861 cggccgacct ggaggctgac ggcgcggagg ctgtcgtgct cgacctggaa caggacgcca
     4921 tcgaggacga cctcagcgac gtggtgagca gcgcggacgc cgttgtgttc gcggcgggcg
     4981 cggggccggg gagcggagcc gagcgcaagc aaacgatgga cttcgggggc gcggtgaagc
     5041 tgatggatgc cgcgaaggcg gccggcgtgc gccgctacgt gatcgtcagc tccatgggcg
     5101 ccgacgaccc ggaatccgcc tcaggtccga tgggtcctta cctcgaggcg aaggcggcgg
     5161 ctgaccgcgc gctcgaggcg agcggtctcg actacacgat cgtccgtccg ggcgggctca
     5221 cgaacgaccc cggcacgggg cggatcgagg cggcgaccaa gctcggccgc cgcggccagg
     5281 tgccgcgtga cgacgtcgcg cagacgctgc tggcaaccct gttcgcgccc acgacgatcg
     5341 gcaagacgtt cgaggtcctc tcgggcgaca cgccgatcca ggaagccgtc cgctcggtct
     5401 agttgccgct ccgcttggtg gcagccagcg cggcctccac ttgctgtctg aacgccatgg
     5461 cagggcgagt ctccgcccgt tacgttgcac ccgtggagcg gtccaggtcc cacctcgcag
     5521 gcgcgatcta cgggcagatc ctcgcgacct cgctcgtggc cactctgtcc gaagacgagt
     5581 ccgcgaccgc cggtgacatc ttcttttggg tcggggtcac gatgctcgtc ttctgggccg
     5641 cgcacgtgta cgcgaactgg cttgcgagac aggtgacgac cggcgggggc ttgagcgagc
     5701 tgggctcggt cgcggccgag caatggccta tggcgcaggc cgcggcgcca gcgcttggcc
     5761 tgctgctgct cggatgggcc ggcgtctact cgcgtgacac ggccgtcgac ctggcgatcg
     5821 ccgccggcat catcagcctt accgcctggg gagtcgtgat cgcccgccgc gccgacgcca
     5881 accagctcga gacgctggcg acggctgtga tcagcgccgg cttcgggttg ctcatggtcg
     5941 gcctgaagat cctggtgcac tgagcggtgc cgaagtggat gggcgtggac gtcggtggca
     6001 agcgcaagca gttcgacgtt gcgctgatcg acgaatcgcg cctgctcgag ctcctagaca
     6061 ggcagcgggt cgaggatgtg gtggcctggg tggattcagt gcgcccgacg atcgtggcga
     6121 ttgacagtcc gcgctcatgc gcgccgccgg gacacggcgt tcgcccggac gagcgcgcgc
     6181 tgcgcgcggc ggtgtgcggt atccgttgga cgccgtcgcg cgaccgactc gacgggagcg
     6241 actactacga gtggatccgc gaaggtctcc agttgtacga ggcgctcgaa ggtctcgcgg
     6301 ccgaggtgat cgagtgcttt ccgacggcgg cgtggacgcg ctggtccggc gaacgaggcg
     6361 gtcgagcgcg ctcgtcgtgg acgcgcgaag cgctcgcgcg actcgagctg gctgacgtgc
     6421 cgctgagaac caatcaagac cagcgcgacg cgatcgcagc cgcagtcaca gcccgcgagt
     6481 acgcgcacgg gcgctgcgag tgcttcggcg agatcgtggt gccgatgcgt cggcagccaa
     6541 gccggagagg ggactcgaac ccctgaccca cggtttacaa aaccgttgct ctaccagctg
     6601 agctactccg gcacgctgat tccagagcct agcgacggcc ttgggttcga ggccgtctac
     6661 cgaatgtcta ccgagaaagg cttggatcgt ctcgcgggtg cgcctcgcgg tcacgtcgag
     6721 acgtgaaggc acgccagctt cgcccatgtt gccgaagctg gcgtggacag cggatgactt
     6781 ggcacgtctg ggcaacttcg cgtggccgac cgtgctcgcg gcgcttgaca tgtgcgcgga
     6841 acgccccggc gtttggatcc cgcttcgcga gatcgaggag cgatccggga gaaaccgcaa
     6901 ccaggctcgt tcggaccttg ctgaactgag acgaagggtg aaacgcgagt tcggccgctc
     6961 taattggcct ttcgtagcgc ggtgggcggc gggtggcgag aagcagttgt attacggcct
     7021 cgatccgccg ctggcgacgg cctggccagc gctctcgcgg actcgagcaa gcggcgtgtt
     7081 ccgggctgtc gttctcgatc ccggtgctcc ggtgagcgga agcgcgtctg cccagtagtc
     7141 gccgcatata gggctcgcgt aggccgcctc ccgcgcgtcc aggagcggat ttccctcaga
     7201 ccgcgctgcg caaggccgcg ccggaagggc tcgagcgcca ctcgccgcgc tcgagccaga
     7261 cgcgggatcc ccagcgccta gaggaggctc ggcgattcga ttcccggctg acgtgagcgc
     7321 cgctgcggcc ggctccggat cgtctatggg ccgcttggtg ggcagacgag cttcgccgtt
     7381 cccttgcgcg gtccctccgg cttgctctcg ggaaagcgcc aggcgaggcg ggtgaacagg
     7441 gagtgaggca cgcagccacc agtcatccgc ggcaggaatg cgtgtagacg gaccggatac
     7501 gtcctgaagg tgcccccagc gcagttggga ttgcagtcgt tgacgagcgc cgtccctcgg
     7561 cccctcgcct cggtctgcgt ccagcgcgac cagacgatgc cgcgcacacg gaaattcgcg
     7621 tccgcacagg tgacgatcac cttcctcggc ttgtatctcc cgcgctggca gttgtagagc
     7681 agcagcaccc gagcctgccc ggccgtagcc acggcaggag atcccccgct ctgcccgccg
     7741 gcggcgccgg cctggcaggc gaaggccgcg atcaccgcca ccagcagtcc ggcgaacgtg
     7801 aggcgacgag aactcgacat gcgctcctcc ttcggtcgac cgtcgtcgtc cagagccgag
     7861 gcccagagtc tcggctacga agcagagtgc gtcaacgcgt tcaccatcaa cgacatttcc
     7921 tctacacgtt cctcaaggtc ccactttgga cgagtatggc tatcccgccg ctcggagcct
     7981 cttcccggct gaccgattgc ttgtccccga caccgtgggg ccgccctccc cgcccgccag
     8041 cggaacactt gggggcggct ccatctaacg gcgtgcgcgg ctcggccggc aacaaggtgg
     8101 tctggaacag ttgtccgaga gggtcgccgg gcttcgcaac cacgcttttg cgctgacggc
     8161 cctgcgatcg tcgaggtcag ttcctggcgt tggcgctccg gatccgacag gaatcggtgt
     8221 ttgagatcga gcgtgggcgc ggctgcgagg tggcgctccc ggctattcgt ccattgcgat
     8281 agccgggccg ctccccgcgt cgaagccggc gcggccccgc atccacgcga ggggaacagg
     8341 cgcattagct ggtgcctgag ccgctcggag ccgctgctga gattcgtcca cagcgatagc
     8401 gaccgcgtct ggttctagac gcccgctatt tggctgggtg gggtgggcac ccatattcgt
     8461 actcgagttc gtggagttga ccaccccacc ccatgtccga tccgcgcggg tccagctggt
     8521 cggcgtatgc ggctcaaggt gcggcgcccc cgccagagtg agcttccgcg ggatcggcta
     8581 cttcgggagg agcagcaggc agaccgcggt ctgctgcatc gcgcgtaggc gggcgaggag
     8641 ggcaccgctg ggcgcgggga tgttgagcga cgttgtgacc gctcggctgc cgtcgcgcgt
     8701 ggccgccgcg aactgtacgt agccggggaa gttgccggtg tggccgtaga cgtttccgca
     8761 cctggtgcgg tagcggaaga cggcgaggcc ggcggagttc cggccgggcc cgggcgggct
     8821 cgactcgccg cccgcgacga agcgcagctg acggcgttgc agcgatgccg ggaagaggcg
     8881 agccccgagg tagccgcgga tgaacgtgcc gaggtccctg ggcgtcgaca ctatggcgcc
     8941 ggaggaccac gctcccgacg ggctgaggag cgtgctcacg tcctcgggtg gggagcccgg
     9001 ttcgaccagg tagccgtgta tgaacggctg cggaagcggc gggttgctcg ggaagctcgt
     9061 gcgggtcagc tcgagcgggt tgaagacaat ctctcgcagc agccggccat agggccgacc
     9121 gccggcttgc tcggccatga ggccgacgac aatgttgtcg gtattcgaat actcgtagcg
     9181 gctgcccgga gtgaagacga gcggctccct ctcgacccag ctcaggatcg tcgcaggcga
     9241 cacgtggccc tggggatcgc tttcggcctg catcctgaag ccgtccgact gcgtgtagtc
     9301 gggcaggccg ctggtgtggt tgagcagctg ctgcaacgtc acggcggacc aggcggaggg
     9361 gaggctgggc agccgctcgc cgatcgtctc gctcagctcg agcttcccgc tggccactag
     9421 ccgcagcgcg actgcgccgc tgaacgcctt ggccacgctc gcgatccgca tgtgatcggt
     9481 cgggcggggt tttctgccgg tctcgacgtc ggccaccccg gcgcgaagtg tcgtcatgcg
     9541 cgatccgcgg cgcagcgtca cgatcgcgcc cggcgggccg ccgtcggccg ccacaagggc
     9601 ctcgagctgg cgctgaaggc cggctttcgt cacgccctgg gcaatcgccg ctggggctac
     9661 gaaggcgcag gcgactgcta ccagggcggc ggcaagtccc gcccgggttg atcgcatggc
     9721 cgccgagtat ctgagttcac acgtcccctg tcaacggggg cggctgacgc ttcgtggcgc
     9781 tcttgtcgcg gggtgattct cgcgcggcgg tgaaactcat ccgcaaacac atcgagggga
     9841 cggagtacat cctggcgggg ctgttcccgg gctagcgcgt aggcggcgtg accggacggg
     9901 gatcggcgct tccggtgtac gaagctgaac cttgatccgg tgggtcgcga ggtgatagag
     9961 agcgcccgac agcccttcgc gccgccggca gccgatgaca agccgtgcga ccgaagcgga
    10021 acgatccgag gaaagaagga tcccgtgcct tgttttcgcg tgttcttggt cgcctcggcg
    10081 gccgtgctcg ggtcactgct cttcgcggcc gggtcggccg ctgcggcgtc agggcccaag
    10141 gtgaccaccg tcgtgtcggg tctggataac ccgcgtgacc tggcgtttgg gccacgcggg
    10201 cgcctctacg tcgccgaggc cggccacggc ggcagcgagt gcgtatccgc tggtgaggag
    10261 ggcacggcat gctttgggtt cagcagcggc atcagccgcg tgaacatcca ggccgggagg
    10321 agccatcgcg tcgtgtccgg gctggcctca accgccgagg agacgggctc ctccgcgaca
    10381 ggcgtcgacg gcatctcgtt cctgggcaac ggcagcatgt tcgggatcat ggccgggtcg
    10441 cgcgacgcca tcccgcccgg gttgtccgcc gcaacgacca caaagttgca gcagcaggcg
    10501 ggccgtctca tcagggtgac gcccgggcgc ggggtcaggt tcggcgcgga cgtcggccac
    10561 gaagacttca catggagcgc tgctcacggc gacctggtcc cgggtcagtt ccccgacgcg
    10621 aacccgtacg gcgtgctcgc gttgcccgga gccacctggg tcgtcgatgc cggctcgaac
    10681 acgatcgacc gcatcgacgc gaggggcaag gtgcacgtcg tggcgttcat ccctaatccg
    10741 ccgtcctccg acgcggtgcc gacatgcatc gacagaagcc gtcacggcgt cctctacgtc
    10801 ggtgagctca ccggcggcgg caaccagcca ggcgcgtccg tggtgtggcg cttcacgccg
    10861 cggggacaca agctgaccca gtgggcgacg ggcctgactg cggttaccgg ctgcggcttc
    10921 gctcacaacg gcaagttcta tgcaaccgag ttctcgacgg cggggctcga caacgcggcg
    10981 ccgggcacgg gcgcggtcgt gcgcgtcgcg ccgcacagca cgtcgcccag gacggtggtg
    11041 tcgaacctca acttcccggg cggattcgcc gccggcggcg acggatcgct ctacgtgtcc
    11101 aactggagca tctcacccgc cggcggcggc gagatgccga gcggcgaggt cctgcggatc
    11161 gcgctccggg cgaagcaccg ctgacagcgc ggcgacctcg agcgcggcgt ggtcgtgaac
    11221 gagaccgacc tgccggacca ggtcgtgagg gaggcggtcg atacctacat cgaggagcac
    11281 gcctcgatgc cgggcgtcaa ccaggcgaca cgtaactgcg gagctatgtg aacaacgcgg
    11341 gctcgctgct cgccggcacg aagtactgct cgccgcgcaa taagagagag gacgcccgtg
    11401 agttgtggga gccacctcgg ttccggttat cggcttcacg gttgtgccgc ctgaggcgtc
    11461 tagcgtgcgc gttctgactc cgtgggtggc agaagcgtgg gtcacatagt gggccgggcg
    11521 cttcgcccct caggccccgc agatcaccta tcgagccgat acgatcgtct cgatagaggt
    11581 ggcacgcact ctggaagtcg aacgggtcta ccgacacttc tgcatggagg cgcgggcgct
    11641 ggaagtggtc ggcgagcgct ggtcgcttct gatcgtccgg gacctgttgc ccggcccacg
    11701 ccggtttacg gatctgatcc gcagcctgaa cccgatcact ccgacccggc tgacggaccg
    11761 gccccggcgg ctcgaggccg cgggggtggt cgagcgtgag ccggcagagc gcggcaacga
    11821 ggtctggtat cggctgacgg atgccgggcg agacctcgga ccggcgatcg acgagctgac
    11881 cttctgggga atacagcacg ccacggagcg cccacgagcc gatgagccgg ttcacggcga
    11941 ggcggcgatg attggcacca aggtctggtt gaacaggtac tcgtccgccc cgccgggcgg
    12001 gctggtctgg gtctggcgat tcccgggcga ggacagctac agcctgcgag cgacggacgg
    12061 tgcctgggat ctgactcgcg gcgagcagga gggtgcggcg gtgacggttg acgcgacgac
    12121 ggcggactgg gcggtcttcc tcacctcacc ccgggagaag cgacggctgc cgaccaagga
    12181 cgtccggctc gagggatcca aggccgaggt gacgaggttc gtcaaggcct ttgcggcgaa
    12241 acctgcggcc cgctgatctc gatcggctcg cacgccacct ctggcggctg gctcgggatc
    12301 acgatcgccg ccgcgaccgc gcccacgagg gtgatggcga ccgccaccag gaccgcgatc
    12361 tggaagccgt cgaagaaagc gttcgaagcg gcctcgtgga gtccctgcgc gagcctcggc
    12421 cggccggtgg catcgaggcg cgcggcgatc ccgaacgcgg tgccgaccga tcgctgtgcg
    12481 ctgtccgcga tcgaaccggg gacagcggcc gggagcgagt cggccaggcg gctggcgtag
    12541 attgacgcgt agatgcttcc gatcacggcg acacccagcg tcgcgccggt gattcgcgtc
    12601 gcgtcgttga ccgccgagcc gacgcccgcc ttggcggcgg acacggctcc catgatcgac
    12661 tcggtcgccg gtgcactggt gagtccgatt ccggacccga tgaagaccat cgaggcggcg
    12721 atcgtcaggt agctggtgct tgtcgatgag gtcgaggccc aggcgagccc gatcgcgagg
    12781 cagaccattc cgctggcgac taccagcttc gtcccgcgcc gcaccgccag cctggtgccg
    12841 agcaccgagg tgatcccggt caacaccgcg accggcagga gcctgacccc ggtggacagc
    12901 ggcccatagc ccttcaggaa ctggaagtag agggtgatca cgaagatgaa tcccatcagc
    12961 gaaaagaatg tgatcgtgac cgcgccgctc gccgcgctga agcgcaggtt cctgaacagg
    13021 cggatgtcca gcagcggctc gcggacgcgg cgttcccagg cgataaacgt ggcgagcagg
    13081 acgccggcca gcgcgaagcc ggcgaggctg aggccggccg cccaccctcg gttgggcgcc
    13141 tcgatgatcg tgtagatcag ggcggccatc gccgccgtcg agagcaccag ccctggccgg
    13201 tcggcgggag gcgcctcggg atcccgcgag gtcgggacgg cgaaggcgac cagcaccgcc
    13261 gcgacggcgg cgaacgggac gagcgcgaag aagacgctcg accaggagaa gcgctcgagc
    13321 agccagccgc cgatgatcgg cccgactgcg atcccgacgc ccgtgctggc gccccagagc
    13381 ccgatcgccc gggcgcgctc cctgcgttct gtgaacacgt tcgtgagcag cgacagggtt
    13441 gccgggaaga tgagcgcggc ccccagcccc atcaccgcgc gcgccgcgat cagctcaccg
    13501 ggactgttcc cgaggccgcc ggcgaagctc gccgcgccga agacgagaag ccctgcgagc
    13561 agcacgccct tgcggccgag ccggtcgccg aggttcccgg cggccagcac gaacgccgcg
    13621 aacatcaggt tgtaggcgtc gacgatccac tcgagctgca ccgtcgaggc gtgaagctcg
    13681 cgcaccagcg tcggcagcgc cacgttcacg atcgtcgtgt cgaggttgat catgaacgcc
    13741 gcgagcagca gcgcgacgag gaccatcggc ttttcgggct cttggttcaa cgcggctgac
    13801 ctccagcgac gtcagagcgg gcgataaacc aggattacca ctccggtgcc tagcgttcgg
    13861 gactcggcga gttccaagct agaggtcggc actccttcgg cgaagagccg ggtgcccgtg
    13921 ccgatcacga gcggatggac ccagatgcgg tattcgtcga tcagattgcg tcccatcagc
    13981 gtggccatca ggccggcgct gccataggtg acgatgtcgc gaccctcctg ccgcttcagc
    14041 tcggcaaccg catcgcccac gtccccctga agcagggtcg cgttccattc gagcggttcc
    14101 ttgagcgtgc tcgaggcgac gaacttgggc atctgattca gggtctgggc gtattcaccc
    14161 tccttcacct ggggccagaa ttccttgaac acctcatagg tgacccgacc gcagaggaac
    14221 gcgtcgcttt cgcgtaggtg ctccagggca taactctggg cctcgtcgtc gaagtagcgg
    14281 ctgccccatc cgccctccgc gagctcctgg aatccacctg ggtcctggat cacgccatcg
    14341 agcgtaacga gggtcgagac attgagcttc cgcattcccg tcttcctatc gacgaccgcc
    14401 cgactccata ctatcgtagt gatactatca tggcgattct agagcgccta ggcagagggg
    14461 aactcaagtg aggagcatcg tcaggtcgga tcacatctcg ctcgacgggg tcgttgaggg
    14521 ggttcggcgc gcgagaaggt aggtagcgga tgcaacagag gggcgttctt gagatcgcgg
    14581 aggcggagcg gcggcgttgg ttggcgctct acgtcctctg cgtcggcatg ctgatgatcg
    14641 tcctcgacgc gacgatcgtg aacgtcgccc tgccgtccat acgggaggac ctgggcttcg
    14701 gtcagaacga cctcgcgtgg gtcttgaacg cgtacctggt tgcgttcggc ggcctgctgc
    14761 tgctctcggg acggatcggc gatctgatcg gccagcggcg cgtcttcttg gtcgggctct
    14821 gcgtcttcag cggcgcgtcc ctgctctgcg cgctctccga gagccagggt cttctgatcg
    14881 gagcccggtt cgtccaggga gtcggcggag cgcttacctc cgcggtcatc ctcgggatga
    14941 tcgtgaccat gttcccggag ccccgggcgc aggcgagggc gatagggatc tacacgttcg
    15001 tcgcggtcgc gggtggatcg gtcggcctgc tgttgggcgg cgtgctcacg cagacaatca
    15061 actggcactg gatcttcttc gtcaacatcc cgatcggcgt cgcgaccgcg ctgctcgcga
    15121 tcaggctcgt tcccgacccc gatgggatcg gcctcgccgc gggcgccgac ctcccgggag
    15181 ccgcgctgct cactggcggc ctcatgctcg gcgtctacac gatcctcggg gtggccgaag
    15241 aagggtgggg atcgatccag acgctagcgc tcggtgcggt ggcgattggg ctgctcgccg
    15301 tcttcctggc acgccaggcg cgggtggcga atccgttgat cccgcttcgg ctgttccgct
    15361 cccgcaacgt ctccggcgcg aacgtggtcc aggcgctcct cgtcgttggg atgttcggga
    15421 tgttcttcct cggcgcgctc tacatgcagc agatcctggg ctacgacccg ctccaggtcg
    15481 gcctcgccta cctcccgttg actattgcca tgggcacgat gtcctttcgc ttcacggggc
    15541 agctcaacct gcggttcggc ccggaggcga cgctcgcccc cgcgatggcc tttgtcctcg
    15601 ccgggctgct ccttctcgcc cgcacgccga tcgacgctac gtatgtgagc gacctgcttc
    15661 cgccgatggt gctgatcggg ctgggcgccg ggctcggctt cccgtccctg atgaccctcg
    15721 cgatgtcggg cgccacggaa agcgactcgg ggcttgcgtc ggggctcgtc aacaccagcg
    15781 ttcaggtggg cggcgcgatc gggctggccg tgctcgccac gctcgcgacc gagcgaaccg
    15841 acaacctggt cgcggacggc gagcccgcgg ccgcggcgct caactccggc taccacgtcg
    15901 catacctgac cggcgccggg ctggtgctcg tcgcgatcgc ggtggcggtg agcttcctgc
    15961 gcgcgcgcgt cgccgagggc tccccggagc ccgaacccgt tcccgaggtg cccggcgagc
    16021 cggttcccga cgcggccacc gagccgggga cgccctcgct ccgagtcaag ccgctcccgg
    16081 cctacgccca agcggcggtc gcgggctgtc aggagacggg catgcgagtc gtcattggct
    16141 gcggtgggtg cgccggctcg gcgcagtaga gtcgagcgtg agcgatggcg agcgactatg
    16201 cgccgacccc ggtagaggac ttcctcgacc ccgacgatcc gacgctcttt ccccagctca
    16261 cgccggagca gatcgagtac ctggcggaga tcggcaccca gctcacgttc gcgcgcgggg
    16321 acgtcgtgtt cgagcacggc cagcgcgaga ccccgctcta cgtggtccag agcggagcca
    16381 tcgacatcat cgaccacgcg ccggaggggg atcggtactt cacccagtgc agggcgggaa
    16441 cgttcgccgc cgacatctcg atgttcaccg gcgagccgac ccttgccgcc gggtacgcgg
    16501 ccgagcccac ctcgctcgtt gcgctcccgc cggaggaagt acgccgcgtc gtcgcgacca
    16561 cggccgagct aggcgacctg ctcctgcgga cgatggtcac ccgccgagag tggcttcagg
    16621 gccgcggtct gggccagcag cggctgatcg gctcacgatg gtcgggagag tccttcgccg
    16681 taagggagct cttggagcgc aacctcgttc ccttcacctg gcacgacctc gcctccgacg
    16741 ctgacagccg gatcctgatc gaagggcttg gcatcgggga ggccgagtgc ccggtcctgg
    16801 tccgctccga cagcgtcatc cggcgagcga ctgtgacctc ggtcgccgat gagctcggac
    16861 tgcgcgcgca ggtcgatggc cagagcttcg acgtcgtcgt gctcggcggg ggcccggccg
    16921 ggctcgccac cgcggtctac gcgtcctctg agggcctcag cacgctcgtg gtcgagcgct
    16981 tcgccccggg cggccaggcg ggcacgagcg cgcggataga gaacttcctc ggctttccga
    17041 cgggcctcac cggcgccgag ctcgcccggc gggcgacgct ccaggcgcga aagttcggcg
    17101 cggtgctctc gagtgtccat gaggcctgcg agatctcgga gccgcaggac ggcttccgcg
    17161 agatcgggct ggccgacggt caacgcgtcc gcggccgctg cgtcgtcctg gcgcccggag
    17221 cggactatcg ccggctgccc gcggacaacg cggatcggtt cgagggcctg ggcctttact
    17281 acgcggcgac gcacatcgag gcgcagcagt gctcggagga ggacgtcgtg gtcgtaggcg
    17341 gagcgaactc ggccgggcag gcagtggtca agctggccga ccacgcgcgc gaggtccacg
    17401 tcgtggcccg gcgaccgctc gaggcggcga tgtcgcgcta cctggtcaac cagatcgagg
    17461 ccaagtcgaa cgtccgcgtc tggtccgggt gtgaggtgcg atcgctggag ggggacgaga
    17521 agctcgaatc cgtggtgatc tcgggtgcgg gtcccgaccg ccgggtggag acctcggcgc
    17581 tgttcgccat gatcggcgcc tccccgaggt cgcaggggct ggtccagttc gtgggccagg
    17641 acgacaaggg cttcgtggtc acgggcgagg aggccaggcg ccaccccgac ttcccccggc
    17701 actggaacca ggagggccgc gggccgctcc tgctcgagtg cacccgcccg ggcgtctttg
    17761 cggtcggcga cgtccgcaca gggtccacaa agcgcgtggc ggcggcagtg ggagacggcg
    17821 cgctggtcgt tcgctccctc cacaccgcgc tcgcttcagg tcaagcgcag gtcaacgacg
    17881 acaggaagct tcgcgccccg gcgccgcaga ccacgccc
//
LOCUS       DASVXS010000051        14044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_507905, whole genome shotgun sequence.
ACCESSION   DASVXS010000051 DASVXS010000000
VERSION     DASVXS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507905"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VF752_02760"
     CDS             complement(<1..480)
                     /locus_tag="VF752_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854493.1"
                     /translation="MTEANERVVEQLRRARDTGDDLSAAVQAHLRAAGPGELRDALAQ
                     RLREVELERSSITRRLSSLEPGPSPLEIGVTIATLPLRIGLGVARLALAPARAAVKLP
                     LEMLLGAGGGEERDPPERHAAAERRAASTFASLERLADDSGDASTAVLAERAAAAAER
                     "
     gene            605..880
                     /locus_tag="VF752_02765"
     CDS             605..880
                     /locus_tag="VF752_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854494.1"
                     /translation="MGLITFLIVLVLSGLFVGALARLAFPGREAMTIGETILVGIAGN
                     LAAGLVLFALGLRNAGSILLSVIFAAILVYLIRRSRGGTLTQPPPRR"
     gene            complement(883..1056)
                     /locus_tag="VF752_02770"
     CDS             complement(883..1056)
                     /locus_tag="VF752_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854495.1"
                     /translation="MGTGFGEIVATIVIVIAVLALVALVVTRVSMRTRGRVGSHSEAH
                     ERGRVGRIWGQKR"
     gene            1127..1528
                     /locus_tag="VF752_02775"
     CDS             1127..1528
                     /locus_tag="VF752_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854496.1"
                     /translation="MADKPMRGAVAEARRVVRGETPVHLHLRQRLVAVATFVVVFDVF
                     SSIVIFFLERNAAGTKIHTVFDAVFWTTTQLLTVSSQLPNPVSTAARVYDFLLQAVAI
                     SVVATLAGSFGSFFNHRSIQRHFEETGEKLV"
     gene            complement(1525..3036)
                     /locus_tag="VF752_02780"
     CDS             complement(1525..3036)
                     /locus_tag="VF752_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8854497.1"
                     /translation="MSADTEHAVIASALDATRLAAATILSVDTRGCITDWNRRAEGTF
                     GWSRGEVLGHSLVATIVSPRLRAQNDRELGAFLGSEPGAESRIETVASHRDGREFEAR
                     LVLVPVSLGRTPEFTAFLDDVAVRPPTHDGIEHLRRRHSNVLTSIAEALGDEPREQPP
                     GQLAGALVLVEQLSEPGVPEASEPLDIFSPPAEPPELLEAEQVEVEPEGVEVDPEPLH
                     DEPPAPDPAALPPPGISLAPSETALAVQRQERALVAPEPVPALLEGERLVLCAQPVLN
                     LGTNEISQYELQLKVADDEGRLTPPEALVFAAERYGVVGWIDRWLVRRAAALAGEHQR
                     DGVNVRLALALSGQALLDPDLVQSVERELSANGTDAERLVLQISEAAAVANVERAAEL
                     VSRLRSIGCGTALADVGSSFTSLRHLKRLPVDQLRLHGGVTESLADSRTDRLVLRALV
                     EVAHGVDALVVADLVSAPGTLEVLRREGVDLAQGVLVGRAATAAETWPGADRA"
     gene            complement(3152..3913)
                     /locus_tag="VF752_02785"
     CDS             complement(3152..3913)
                     /locus_tag="VF752_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8854498.1"
                     /translation="MGLTLSDNELLERQFASLRVFVRAIGEGSPGGSVVERPGVTGSV
                     SPAVPTRSIMNSVVYDHAGALEQAWDDLASAYDEAGVTAWTVWVAPGDEDAVRVLRER
                     GHRLDAEPTAMAFELAGFRGEQAIDPDPRPSGVESARLNDEAYGYAGDFERGIGSVME
                     DRGHLYIASADGRPASCAWALDASGDCGIFAVATTPAARGRGLAGGLIARALLDARER
                     GCETSSLQSTRMGESVYERLGYRSLGKLGMWEHRR"
     gene            complement(3921..4343)
                     /locus_tag="VF752_02790"
     CDS             complement(3921..4343)
                     /locus_tag="VF752_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854499.1"
                     /translation="MSQENVEFVKGLFAAASGMDKEALLSALPELIAQTCDPEIEWHE
                     DPQRPDARIYRGHDGVRESFERWLDEWDEYGTEGERFIDCGDDVLAVARERGRGTRSG
                     VTVSARIFIVVTFRDGKVLRYREFYDEEAAFKAAGLRE"
     gene            4545..5048
                     /locus_tag="VF752_02795"
     CDS             4545..5048
                     /locus_tag="VF752_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEX8854500.1"
                     /translation="MAHELEIRNTEDRVKVRSPWAVALLPIITLGIYHLVWWYRVNRE
                     MRDYGRAKGYDLGQNPTASVLALFPGGIIIVPALISYWRGTKRVQGAARIAGKEPPNG
                     WIALILYLLIALGFYAYLQVSLNDIWRSEAEPLRGQEPLPPAPDTMPPRLEDQQQAPS
                     EQPQPNP"
     gene            complement(5097..5975)
                     /locus_tag="VF752_02800"
     CDS             complement(5097..5975)
                     /locus_tag="VF752_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8854501.1"
                     /translation="MLRARPFLVLSLACWALLLLVYVFALGTGPGRAADRHTLLNGPA
                     GPRWQEVHDASSHLVHTIDVSSLVLAAVAIVGIALVRRLPGHAIAAAVTIVGAVGTTE
                     VLKPGLGALDPLGENADRAIGSSFPSGHATVAMSVTLALLVVVPPAWRPAAAVLAALY
                     SGVVGIGLVLLGAHFPSDVAGGYLVACAWASAACAGLAEWTRRHPEVDAVPGSRAIAA
                     GATAALSVAFVIVLLVALAAVRSTDLGKLEPLGRLHTSFFATSGGLLVLAALVPAVLA
                     AMLLRGHLRAGSALHV"
     gene            6033..7280
                     /locus_tag="VF752_02805"
     CDS             6033..7280
                     /locus_tag="VF752_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8854502.1"
                     /translation="MRQPPDHLKALVERYDPKVFDAPTGRARIRLSVAGNGDWDVVVR
                     GRKAELRESSPRADPDAQLVADASTWRRIAADVRGGMAAFEQGRLVIRKDLHLGVGLL
                     AATSGMTDEGRLRFARVKTNKGRICTASAGQGDPPVLLIHGLGGTKASFLPTVAALAA
                     DHRVIALDLPGFGDSDKPLAAPYDAPWFARSVVGLMDALKIERAHVIGNSMGGRVALE
                     LGMSYPERVDKLVLVTPSLAWMRERSWAPLLRFVRPELGLLQLAPRRVVEGVVRRLVP
                     GARDGWAAAGVDEFLRAYLTPRGRAAFYAAARNIYLEEPDGETGFWTRLAGLEPEALF
                     IWGHRDTLVPIGFKRHVSEAVPHAKHAELDCGHVPQVERPRETHAAIKRFFADTRRDS
                     ASGPGRARGSGARRRGSSRSIRG"
     gene            7370..7798
                     /locus_tag="VF752_02810"
     CDS             7370..7798
                     /locus_tag="VF752_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8854503.1"
                     /translation="MRFIVLVKATEDSEAGVMPSQQLLSDMGNFNEELVKAGVMLAGE
                     GLHPSSKGVRIAFDGDERRVIDGPFAETKELVAGYWLWQVKSLDEAIEWAKRIPNPDG
                     KGGGVVEIRQVFEADDFGEELTPELREQEERLRREAAANQ"
     gene            7806..9095
                     /locus_tag="VF752_02815"
     CDS             7806..9095
                     /locus_tag="VF752_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8854504.1"
                     /translation="MPTIRPSSSNVNRTIEAVWRIESARLIAGLARLVRDIGLAEDLA
                     QEALVAALEQWPESGVPDKPGAWLMGTAKHRAIDRLRRADVHERKTQELAREAEIGGQ
                     AENPDLDAEIDDPFDDDLLRLIFTTCHPVLSTDARVALTLRLLGGLTTKEIARSYLVS
                     ESTVAQRIVRAKRTLSAARVPFEVPERDELPDRLASVLEAIYLIFNEGYAATAGDDLI
                     RPELIEESLRLGRILAELLPSEPEVHGLVALMELQASRSRARIGPSGEQVLLLDQDRS
                     RWDWVLVSRGLAALERAQSTRRALGPYTLQAAIAACHARARVADDTDWERIVALYDAL
                     AQLTRSPVVELNRAVAVGMAYGPEAALELVDKLRSEPALSDYHLLPAVRGDLLEKLGR
                     IDQARAEFERAAALAQNSRERAVLLDRIAECSPRNGG"
     gene            complement(9101..11563)
                     /locus_tag="VF752_02820"
     CDS             complement(9101..11563)
                     /locus_tag="VF752_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8854505.1"
                     /translation="MIRMIARAIPGALVALLAISPAAGAATRDYADTALNIIPSGQWG
                     GFPLNPKADLQAKMYDGLTPLFGNVKPNDLTKYFKSERFGGKGTVEKVPRKGVRIVRD
                     SYNVPHITGKKRDDVIWGAGWVAAEDRQLLIDQARFDARIAAIDAPGLDAFTLVQQLR
                     QFVPSAQTEAVVARQTNVLKKAGAKGRSLLHDIDVYAAGMNAQYKAAGHPADPPFGRT
                     DVYAVNALAGQLFGRGGGDEVRRSMFLDGLQKRLGSGRGLSVFNDLRNYEDPASPVTI
                     DKAFAGESKMPSGVGAGNAVIDDGSFQRTPAAKLSKSAAPPDQRRSASNFLVVGAKRS
                     STGHPLFVAGPQIGYFYPGLTMEMDLHGGGIDARGATAPGLNPGAILIGRGADFAWSL
                     TSAGSDVIDQYAETLCGASDHKYMYEGKCRNMHFFNAGVIKGSGGAPDQPVTFWTTVH
                     GPVIGYATSKGVRVAISSKRSSYGKDVLWELPFVDASNGRIDSPQTFIKSFAQSPFTF
                     NSVYADDKHISMISTGLFPKRPKSVNPGLITKGNGKFEWQGFLPHKDYPRETDPKDGI
                     LVNWNNRPAKDWPAADDEWGYGSLYRMQMLRNGLVSRRKHTLATVVSAMNRAATQDFR
                     DLAVVPDIAAVLKGSTAPSPRDQKMLDLMLAWRRAGSSRLDRNNDGKVDDPGAAIVDA
                     AWPGLADAVMQPVIGPQLDALASLSPRNPAARGDFQGGWLSYMDKDLRTLAGQKVAGT
                     YRNRFCGGGNKAACQQALWGAIHAAGDTLQAAQGPDPSAWRADANAEKISFAPGLLPF
                     KMRYTNRPTGIQQVISFGGHRP"
     gene            complement(11815..12018)
                     /locus_tag="VF752_02825"
     CDS             complement(11815..12018)
                     /locus_tag="VF752_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8854506.1"
                     /translation="MATGTVKWFNDEKGFGFITPDDGGRDLFVHHTGILGSGFKSLNE
                     GAQVSYEAEQGDKGPKAVNVQPV"
     gene            complement(12185..12664)
                     /locus_tag="VF752_02830"
     CDS             complement(12185..12664)
                     /locus_tag="VF752_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8854507.1"
                     /translation="MSTPASATSIRHDVVVEAPLERAFSVFTDGFGSFKPREHNMLSV
                     DIAETIFEPRVGGHVYDRGVDGSECRWARVLVYEPPHRVVISWDISPHWQVETDLEKT
                     SEVEVRFTAEGPDRTRVELEHRNLDRHGDGWEAVREGVDSEGGWPLYLQRYAEVVAA"
     gene            complement(12661..12981)
                     /locus_tag="VF752_02835"
     CDS             complement(12661..12981)
                     /locus_tag="VF752_02835"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8854508.1"
                     /translation="MQTYQPDTWSALGDRTRRAIFERLAESPSAVGEIAGGLPVSRPA
                     VSQHLKVLKQAGLVVDEPAGNRRIYRVNPDGLVALRADLDRFWRTALTAYKAAVEQTE
                     QEIS"
     gene            complement(13148..13477)
                     /locus_tag="VF752_02840"
     CDS             complement(13148..13477)
                     /locus_tag="VF752_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854509.1"
                     /translation="MATIEQGNGFTPSVEAPQKRTVALELSVPEAEALRAWLLTATND
                     GKTALDDSMVKAATMKLGASIDLISAVVKVREELEGAGFDTADMTDEEVEALGRKISE
                     TSLHRLT"
     gene            complement(13610..>14044)
                     /locus_tag="VF752_02845"
     CDS             complement(13610..>14044)
                     /locus_tag="VF752_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854510.1"
                     /translation="ESVEAADVTWREATSTTASVAARVTTFRADERLHAVAPRSGNWW
                     DHAVMLTVGGHLPYRPRDLFQGMDGQPLERRAKVLAWRLRLTAGALPRLASPHRLFAP
                     VPALATHAEADYLAAGVDWGAVAADIGEWAASRGMELPVIAR"
BASE COUNT         2115 a         4973 c         4766 g         2190 t
ORIGIN      
        1 acgctcggct gcggccgcgg cacgctcggc gagcactgcc gtcgatgcgt caccgctgtc
       61 gtccgccagg cgctcgaggg acgcgaacgt ggacgccgcg cggcgctccg ccgcggcgtg
      121 gcgctccggg ggatcgcgct cctcgcctcc gcccgctccc agcagcatct cgagcggcag
      181 cttcacagca gcgcgcgccg gggcgagtgc aagcctggcg acgcccaacc cgatccgcaa
      241 cggaagcgtc gcgatcgtca cgccgatctc gagcggcgag ggtcctggct cgagcgacga
      301 cagccggcgc gtgatcgacg agcgctcgag ctcgacctca cgcagtcgct gcgcgagcgc
      361 gtctcggagc tcgcctggac cggcggcccg gaggtgtgcc tggacggcgg cgctcagatc
      421 gtcaccggtg tcgcgagcgc gccgcagttg ctcgacgacc ctctcgttcg cctcggtcat
      481 ctctcgagtc tttcccgttc ggggtggggt cgggggtggg gttcgggccc gaatctagtt
      541 gttgccctcg attcgggcgt gtgaatcgcg gatgaccgcg agcacgggtg ggtagcctca
      601 gggcatggga ctcatcacct tcctcatcgt cctggtcctc agcgggctgt tcgtgggcgc
      661 gctcgcccgc ttggccttcc ccggccgtga ggcaatgacg atcggcgaga cgatcctcgt
      721 cgggatcgcc ggcaacctcg cggccggcct ggttctcttc gcgctcggct tgcgcaacgc
      781 gggcagcatc ctgctgtccg tgatcttcgc cgcgatcctg gtctacctga tcaggcgctc
      841 acggggcggc acgctcacac agccgccacc tcgccgctag ggctagcgct tctgccccca
      901 gatccggccg acccggccgc gttcgtgggc ctcggaatgg gagcccacgc ggccgcgcgt
      961 ccgcatgctc acgcgggtga cgaccaaggc caccagcgcc aggacggcta tcacgatcac
     1021 gatcgttgcg actatttcgc cgaagcctgt tcccatgggg gcgcgtctac ccgagaccgc
     1081 cctcctgaac cattcttacg actgccaccg gggccatact cgccgcgtgg ccgacaaacc
     1141 catgaggggg gcggtggcag aggcgaggcg cgtcgtccgc ggcgagacgc cggtgcatct
     1201 acacctgcgg cagcggctgg tcgcggtggc gacgttcgtg gttgttttcg atgtgttctc
     1261 gagcatcgtc atcttcttcc tggagcgcaa cgcggccggg acgaagattc acaccgtgtt
     1321 cgacgcggtg ttctggacca cgacccagct gctcaccgtg tcgtcccagc tccccaaccc
     1381 ggtctccacc gcggcgcgcg tctacgactt cctgctgcag gccgtcgcta tctcagtcgt
     1441 cgccacgctg gcgggctcgt tcggcagctt cttcaaccac cgttcgatcc agcgtcactt
     1501 cgaggagacc ggcgagaagt tggtctaagc ccggtcggcg cctggccagg tctccgcggc
     1561 ggtcgccgcg cggcccacga gcacgccctg ggcgagatcg acgccctccc gcctgagcac
     1621 ctcgagcgtg ccgggcgcgg acacgaggtc cgcaacgacg agtgcgtcca ccccgtgcgc
     1681 gacttccacg agcgcgcgca gcacgaggcg gtcggtgcgg ctgtccgcga gcgactccgt
     1741 cacgccgccg tgcaggcgga gctggtcgac cggcaggcgc ttcaggtggc gaagcgacgt
     1801 gaagcttgac ccaacgtcgg cgagcgccgt cccgcacccg atcgagcgca ggcgcgagac
     1861 gagctccgcg gcacgctcca cgttcgcgac ggccgctgcc tccgagattt gcagcaccag
     1921 ccgctcagca tccgtgccgt tggcggatag ctcgcgctcg accgactgca cgaggtccgg
     1981 gtcgagcagc gcctgcccgg agagcgccag cgcgaggcgc acgttcacgc cgtcacgctg
     2041 atgctcgcct gccagcgcgg ccgctcggcg cacgagccag cggtcgatcc agccgaccac
     2101 gccgtagcgc tccgccgcga acaccagcgc ctcgggcggc gtgagcctcc cctcgtcgtc
     2161 ggctaccttg agctgcaact cgtactgcga gatctcgttg gttccgaggt tgagcaccgg
     2221 ttgcgcgcac aggacgagcc gctcgccctc tagcaaggcg gggaccggct cgggcgccac
     2281 cagcgctcgc tcctgacgtt gcaccgcgag agcggtctcg gacggggcaa gcgagattcc
     2341 gggcggcggc agtgcggcgg ggtccggagc aggcggctca tcatgcagcg gctcgggatc
     2401 gacctcgacg ccttccggct cgacctccac ctgttcggct tcgagtagct ccggcggctc
     2461 ggccggcggc gagaagatgt ccaggggctc gctggcctcg gggacgcccg gctccgagag
     2521 ctgctccacc agcacgagcg cgccggcgag ctgccccggc ggctgctcgc gcggctcgtc
     2581 gccgagcgct tccgcgatcg acgtgagcac gttcgagtgg cggcggcgca gatgctcgat
     2641 gccgtcgtgg gtcggcggcc ggaccgccac gtcgtcgagg aaggcagtga actcgggcgt
     2701 ccgaccgagc gacaccggca cgagcaccag ccgcgcctcg aactcgcggc cgtcgcggtg
     2761 gctcgccacc gtttcgatac ggctctcggc gccgggctcg gatcccagga acgcgccgag
     2821 ctcgcgatcg ttctgggcgc gcaggcgcgg cgacacgatg gtcgcgacga gcgagtggcc
     2881 gagcacttcg ccgcgcgacc agccgaaagt gccctcggcg cggcggttcc aatcggtgat
     2941 gcagccgcga gtgtcgaccg agaggatcgt ggccgccgca aggcgggtcg catcgagcgc
     3001 cgaggcaatg accgcgtgct cggtgtcggc gctcatgagc gctttccggg cttgcgtgga
     3061 cggccgatcc gttgcgacat cgaggtgctc gaaagatagg cgcgcgcgtt ggcgcccctg
     3121 tacctacttt cgtccacgcg gcccggcgcc tttagcgccg gtgctcccac atccccagct
     3181 tgcccagcga gcggtagccg agccgctcgt acacggactc gcccatacgg gtcgactgca
     3241 gcgagctcgt ctcgcagccg cgctcgcgag cgtcgagcag ggctcgcgcg atcaggccgc
     3301 cggcgagtcc gcggccgcgt gcagccggtg tggtggcgac ggcgaagatg ccacagtcgc
     3361 cgctcgcgtc cagtgcccag gcgcacgagg ccgggcgccc gtccgcgctc gcgatgtaca
     3421 gatggccgcg gtcctccatc acgctgccga tcccgcgctc gaaatcgccc gcgtagccat
     3481 aggcctcgtc gttgaggcgc gcggactcca cccccgacgg acgggggtcc gggtcgatcg
     3541 cctgctcacc gcggaacccc gcgagctcga acgccatggc agtcggctcg gcgtcgagcc
     3601 ggtgcccgcg ctcgcgaagc acccgcacag cgtcctcgtc gccgggcgcg acccaaacgg
     3661 tccaggcggt cacgcccgcc tcgtcgtagg cgctcgcgag gtcgtcccat gcctgctcga
     3721 gcgcgccggc atggtcgtac acgaccgagt tcatgatcga gcgcgtcggc acggccggtg
     3781 acacggaccc cgtcacgccg ggccgctcga ccacgctgcc gccgggcgag ccctcgccaa
     3841 tcgcgcgcac gaacacgcga aggcttgcga actgccgctc gagcagctcg ttgtcggaga
     3901 gggtcagccc cacgcggggg ctactcccgg agccccgcgg ctttgaacgc cgcttcctcg
     3961 tcgtaaaact cgcggtagcg caagaccttg ccgtcgcgga acgtgacaac gatgaagatc
     4021 cgggcgctca ccgttacccc gcttctcgtc ccgcgaccac gttcccgggc aaccgccagt
     4081 acgtcgtcgc cacagtcgat gaatcgttcg ccctcggtgc cgtactcgtc ccactcgtcg
     4141 agccatcgct cgaacgattc gcgcacgccg tcgtgaccgc ggtagatacg ggcgtcgggc
     4201 cgctgcgggt cttcatgcca ctcgatctcg ggatcacagg tttgggcgat caactccggc
     4261 agcgccgaca acaacgcttc cttgtccatg cccgaggcgg ctgcgaagag ccccttcacg
     4321 aactcgacgt tctcctgcga catcgcccgt ccagtatctc gcggcggagt ttccgctttc
     4381 catgcgactc gtgagtgtct ccgcagctct cagaggagag cggcgatcac agagtccaat
     4441 atcacctgtt ctgggtgacg cgcccgagat cccccgcgtg gatcgtgccc tgagtagtta
     4501 ccccgccgga gggtcacaag gggccccgga aggagaagtt cgtcatggcg cacgagctcg
     4561 agatcaggaa caccgaggac cgcgtgaagg ttcgcagccc ttgggccgtg gctctgctgc
     4621 cgatcatcac gctgggcatc taccacctcg tctggtggta tcgcgtcaac cgggagatgc
     4681 gcgactacgg ccgcgcaaag ggctacgacc tgggacagaa cccgacggcc tcagtgctgg
     4741 cactgttccc gggtggcatc atcatcgtgc ccgcgctgat cagctattgg cgcggcacca
     4801 agcgagtgca gggcgcggca cgcatcgcag gcaaggagcc gccgaacgga tggatcgcac
     4861 tgatcctcta cctgctgatc gcgcttggct tctatgccta cctgcaggta tcgctgaacg
     4921 atatctggcg gtccgaggcg gaaccgctgc gcggacagga gccgctcccg ccggcgccag
     4981 acaccatgcc gccgcggctt gaagaccagc agcaggcgcc gtctgagcag ccgcagccga
     5041 atccctgagt tcgtctatcg cgtcgggagc ccggcccggg ggccgagctc ccggcctcac
     5101 acgtgcagcg ccgaccccgc gcgcagatgc ccccgcagca acatcgccgc gagaaccgcg
     5161 gggaccagcg ccgcgagcac cagcagtccg ccgctcgtcg cgaagaacga cgtgtggagg
     5221 cgccccagcg gctcgagctt gccgaggtct gtgcttcgca cggcggcaag cgcaacgagt
     5281 agcacgatca cgaacgcgac gctcagcgcc gccgtcgccc ccgcggcgat cgcacgcgag
     5341 ccaggcaccg catcgacctc cgggtgccgc cgggtccact cagcaagccc ggcgcaagcc
     5401 gcagacgccc acgcacaggc gacgaggtag ccgccggcca cgtcgctcgg gaagtgcgcg
     5461 cccagcagga cgagcccgat cccgacgacg ccggaataca gcgccgcgag caccgccgcg
     5521 gcgggcctcc aggcgggcgg cacaacgacg aggagcgcga gcgtgacgga catcgccaca
     5581 gtggcgtggc cgctcgggaa gctcgacccg atcgcacgat cggcgttctc gccgaggggg
     5641 tcgagcgcgc cgagccccgg cttcagcact tcggtcgtcc cgacggcgcc gacaatcgtg
     5701 accgcggcgg caatggcgtg cccgggcagg cgccgcacca gcgcgatgcc cacgatcgcc
     5761 acggctgcca gcacgagcga gctcacgtca atcgtgtgga cgaggtggct cgaggcgtcg
     5821 tggacctcct gccagcgcgg cccggcgggg ccgttcagca gcgtgtggcg gtccgccgcc
     5881 cggcccggac cggtcccgag cgcgaacacg tagacgagca agagaagcgc ccagcaggcg
     5941 agcgacaaca ccaggaacgg acgggctcgg agcacctgtt cacgataggt cgtcgaaact
     6001 gtccgctgcc ttctctgata tccaccacga cggtgcggca gccaccggac catctcaagg
     6061 cgctcgtcga gcgctacgac cccaaggtct tcgacgcccc gaccggacgg gcccggatcc
     6121 gcctctcagt cgcggggaac ggcgactggg acgtagtcgt gcgcgggcgc aaggcggagc
     6181 tccgcgagtc gagcccgcgc gccgatcctg acgcccagct cgtggccgac gccagcacct
     6241 ggcgccgcat cgcagcagat gtgcgcggcg ggatggccgc cttcgagcag ggccgcctcg
     6301 tgatccgcaa ggacctgcac ctcggcgtcg ggctgctcgc cgcgacgagc ggcatgactg
     6361 acgaagggcg cctgcgcttc gcgcgcgtga agacgaacaa aggccgcatc tgcaccgcga
     6421 gcgccgggca gggcgatcca cccgtgctcc tcatccacgg attgggcggc accaaggcct
     6481 cgttcctccc caccgtcgcc gcgctcgccg cggaccaccg tgtgatcgcc ctcgacctcc
     6541 ctggcttcgg cgactccgac aagccgctcg cggcgcccta cgacgcgcca tggttcgcgc
     6601 gctcggtcgt cggtctcatg gacgcgctga agatcgagcg ggcgcacgtg atcgggaaca
     6661 gcatgggcgg tcgcgtggcg ctcgagctcg gcatgtcgta ccccgagcgc gtcgacaagc
     6721 tcgtgctggt cacgccctca ctggcctgga tgcgcgagcg atcctgggcg ccgctacttc
     6781 ggttcgtgag gcctgagctc ggcctgctgc agttggcgcc gcgacgcgtg gtcgagggcg
     6841 tagtgcgccg cctcgtgccc ggcgcgcggg acggctgggc ggccgcgggc gtcgacgagt
     6901 tcctgcgcgc ctacctgacc ccgcgcggcc gcgccgcctt ctacgccgcc gcgaggaaca
     6961 tctacctcga ggaacccgac ggcgagaccg gcttctggac gcggctggcc ggcctcgagc
     7021 ccgaggcgct cttcatctgg ggccaccgcg acacactcgt gccgatcggc ttcaagcgcc
     7081 acgtgtcaga agccgttccc cacgcaaagc acgcggagct cgactgcggc cacgtgccac
     7141 aggtcgagcg cccgcgcgag acgcacgcgg ccatcaagcg attcttcgcg gacacacgcc
     7201 gcgacagcgc ctcagggccg ggtcgcgcac gcggcagcgg cgctcgacgg cgagggtcct
     7261 cgcggtcgat ccgcggctag cgtcggacgg gccagcgccc gcgtgtcgat tttcgcgtag
     7321 ctcgctcgat gcataggtag agacctacca acccaaggag gacgtaccaa tgcgattcat
     7381 cgtcctggtc aaggccacag aggacagcga ggcgggcgtg atgccgagcc agcagctcct
     7441 ttccgacatg ggcaacttca acgaggagct cgtcaaggcc ggcgtgatgc tcgccggcga
     7501 ggggctgcac ccgagctcca agggcgttcg gatcgccttc gacggcgacg agcgcagggt
     7561 gatcgacggc cctttcgcgg agaccaagga gctcgttgcc ggttactggc tctggcaggt
     7621 gaagtcgctg gacgaggcga tcgagtgggc caagcggatc ccgaatcccg acgggaaggg
     7681 cggcggcgtg gtcgagattc gccaggtgtt cgaagccgat gacttcggcg aagagctcac
     7741 tcctgagcta cgggagcagg aggagcgctt gcgcagggag gccgcggcaa accagtaggg
     7801 cgccggtgcc gacgatccgc ccttcatcga gcaacgtcaa ccgcacgatc gaggccgtct
     7861 ggcgcatcga gtcggcgcgg ctgatagcgg gtttggcgag gcttgtacgc gacatcggac
     7921 tcgccgaaga cctggcccag gaggcgctcg tcgccgccct tgagcagtgg cccgagtcag
     7981 gcgtcccgga caaaccgggc gcctggctca tgggcactgc gaagcaccgc gcgatcgacc
     8041 ggctgcgccg agccgacgtg cacgagcgca agacccagga gctggcgcgt gaagccgaga
     8101 tcggcggcca ggccgagaac ccggatctcg acgccgagat cgacgatccg ttcgacgacg
     8161 acctcctgcg cctgatcttc acgacctgcc atccggtgct ctccaccgac gcacgtgtcg
     8221 cgctcacgct gcgtctgctg ggtggcctga cgaccaagga gatcgcgcgc tcgtacctgg
     8281 tctccgagtc gaccgtcgca cagcgcatcg tgcgcgcgaa gcggacgctt tcggcggcac
     8341 gggtgccgtt cgaggtgccc gagcgcgacg agctgcccga ccgcctcgcc tcggtgctcg
     8401 aggcgatcta cctcatcttc aacgagggct acgcagctac agcgggcgac gacttgatcc
     8461 ggccagagct gatcgaagag tcgcttcggc tcgggcgcat cctggcggag ttgctgccgt
     8521 ccgagccgga ggtgcacggc ctggtagctc tcatggaact gcaggcgtcc cggtcgcgcg
     8581 cgcgaatcgg cccgtcagga gagcaggtgc tgctgctcga ccaggaccgc tcgcggtggg
     8641 actgggtgct cgtgagccgt ggactagccg cgctcgaacg cgcgcagagc acgcgccgcg
     8701 ccctcgggcc gtacacgctc caggcagcga tagccgcctg ccacgcgcgc gcccgcgtgg
     8761 ccgacgacac cgactgggag cggatcgtcg ccctctacga cgcactcgcc cagctcacgc
     8821 ggtctcctgt cgtcgagctc aaccgggcgg tcgccgtcgg catggcgtac ggcccagagg
     8881 cggccctcga actggtggac aaactcaggt ccgagcccgc gctgagcgac taccacctac
     8941 tgcccgccgt gcgaggagac ctgcttgaga agctaggccg aatcgaccaa gcgcgcgccg
     9001 agttcgagcg cgccgcagcg ctcgcgcaga actcgcgcga gcgtgcggtg ctgctcgatc
     9061 ggatcgcgga gtgctcgcct cggaacggag ggtgaccgcc ctacggccgg tgcccgccga
     9121 agctgatcac ctgctggatc ccggtcgggc ggttcgtgta gcgcatcttg aagggcagca
     9181 gccccggcgc gaagctgatc ttctccgcat tcgcgtccgc cctccacgct gagggatccg
     9241 gcccctgcgc agcctgcaac gtgtcgccgg ccgcgtggat cgctccccat agcgcctgct
     9301 ggcacgccgc cttgttgcca ccaccgcaga agcggttccg gtacgtgccg gccaccttct
     9361 ggccggcgag cgtgcgcagg tccttgtcca tgtacgacag ccaccctccc tggaagtcgc
     9421 cgcgcgcggc cggattgcga gggctcagtg aggcgagcgc gtcgagctgc ggcccgatca
     9481 ccggctgcat aaccgcatcc gccaagcccg gccaggcggc gtccacgatc gccgctcccg
     9541 ggtcgtcgac cttgccgtcg ttgttgcggt cgagccggct cgagcccgcc cgacgccacg
     9601 cgagcatcag gtcgagcatc ttctgatcgc gcggactcgg cgccgtgctc cccttcagga
     9661 ccgcggcgat gtccggcacc accgcgaggt cgcggaagtc ctgcgtggcg gcgcggttca
     9721 tcgccgacac gacggtcgcg agcgtgtgct tcctcctgct cacgaggccg ttccgcagca
     9781 tctgcatgcg gtagagggag ccgtagcccc attcgtcatc ggccgccggc caatccttcg
     9841 ccgggcggtt gttccagttc acgaggatcc cgtccttcgg gtcggtttcc cgcgggtagt
     9901 ccttgtgcgg caggaacccc tgccattcga acttgccgtt gcccttggtg atcagcccgg
     9961 ggttcacgct cttcggccgc ttcgggaaca ggccggtcga gatcatcgag atgtgcttgt
    10021 cgtcggcgta cacggagttg aaggtgaacg gcgactgcgc gaacgacttg atgaacgtct
    10081 gcggcgaatc gatccggccg ttgctcgcgt cgacgaacgg cagctcccag agcacgtcct
    10141 tgccgtagct cgagcgcttc gaggagatcg ccacgcgcac gcccttcgac gtcgcgtagc
    10201 cgatcacggg accgtgcacg gtcgtccaga aagtgaccgg ctggtccggc gcgccgcccg
    10261 agcccttgat cacgccggcg ttgaagaagt gcatgttccg gcacttgccc tcgtacatgt
    10321 acttgtggtc gctggcgccg cagagggtct cggcgtactg gtcgatcacg tcagagccgg
    10381 cggaggtgag gctccaggcg aagtcggccc cgcggccgat caagatcgcc cccgggttga
    10441 gtcccggcgc ggtggcgccg cgcgcatcga tgccgccgcc gtggaggtcc atctccatgg
    10501 tcaggcctgg gtagaagtag ccgatctgtg ggcccgccac gaagagcggg tgcccggtgc
    10561 tcgagcgctt ggcgccgacc accaggaagt tgctcgcgct gcgccgctgg tccggcggag
    10621 cggccgactt ggacagcttc gccgccggcg tgcgctggaa gctgccgtcg tcgatcacgg
    10681 cattgccggc gccgacgccg ctcggcatct tcgactcgcc cgcgaacgcc ttgtcgatcg
    10741 tgaccggcga ggccggatcc tcgtagttgc ggaggtcgtt gaacacggag aggccgcgcc
    10801 ctgagccgag ccgcttctgc agcccgtcga ggaacatcga gcgccgcacc tcgtcgcctc
    10861 cgccgcggcc gaagagctgg cccgcgagcg cgttgaccgc gtagacgtcc gtgcggccga
    10921 acggcggatc ggcaggatgc cccgccgcct tgtactgcgc gttcattccg gccgcgtaca
    10981 cgtcgatgtc gtgcagcagc gagcgaccct tcgcgccggc cttcttcagc acattggtct
    11041 gtcgcgcgac gaccgcctcc gtctgcgcgc tcggcacgaa ctgccggagc tgctggacga
    11101 gcgtgaacgc atcgagcccg ggagcgtcga tcgccgcgat ccgcgcgtcg aagcgcgcct
    11161 ggtcgatcag gagctgacgg tcctcggctg ccacccagcc ggcgccccag atcacgtcgt
    11221 ctcgcttctt gccggtgatg tgcggcacgt tgtacgagtc gcgcacgatc ctcacgccct
    11281 tgcgagggac cttctcgacg gtgcccttgc cgccgaagcg ctcagatttg aagtacttgg
    11341 tgaggtcgtt cggcttgacg ttgccgaaca gcggcgtgag gccgtcgtac atcttggcct
    11401 ggaggtcggc cttcgggttc agcgggaagc cgccccactg gcccgacggg atgatgttga
    11461 gcgccgtgtc ggcgtagtca cgcgtggccg cgccggcggc gggggagatg gccaggagcg
    11521 cgacgagcgc gcctgggatc gcgcgggcga tcatccggat catgcacctg ctcctttgcc
    11581 tcggaactga acgagtccct cagtccccct ctgagagcgg gagcaggtta acagcaaacc
    11641 tgacgattcc gtcatctaaa gaaagagcgc gccaaccggc gcgctctctc gtcagtgtcc
    11701 tgcgaagtgg ttaggcgagg cagctgactg aatcgcccta cgattcagat ggctcccgct
    11761 aactgacagt cgcgctagcg cgcgaagccg tagggagccg gctgaatcgt cgtcctagac
    11821 aggctggacg ttgacggcct tcgggccctt gtcgccctgc tctgcctcgt agctcacctg
    11881 cgcgccctcg ttcaggctct tgaagcccga gccgaggatg ccggtgtgat ggacgaagag
    11941 gtcacgtccg ccgtcatcag gagtgatgaa gccgaagccc ttttcgtcgt tgaaccactt
    12001 cacggttcct gtggccatgt gttctgttcc ctccaggggt atgtgccgga catccggagg
    12061 gcgttacacg acccacagtc tgcctgcacc tgctttgccg gacgggcctg tccggctcct
    12121 tctaatgcgc aaggtagcag cggtcggcga actttgcagt attcgaacga agacgtgccg
    12181 gcgctcacgc agcgacgacc tcggcgtagc gctgcaggta cagcggccag ccgccctcgg
    12241 agtcgacgcc ttcgcgcacg gcctcccagc cgtcgccatg gcgatcgagg ttgcggtgct
    12301 cgagttcaac ccgcgtgcgg tccgggcctt cggcggtgaa gcgcacctcg acctcactgg
    12361 tcttttcgag gtcggtctca acctgccaat gcgggctgat gtcccagctg atgaccacgc
    12421 gatgcggcgg ctcataaacc agtacgcgtg cccagcgaca ctcgctgccg tccactccgc
    12481 ggtcatagac gtggccgcca acgcgcggct cgaagatcgt ctcggcgatg tcgacgctga
    12541 gcatgttgtg ttcgcgcggc ttgaagctgc cgaagccgtc ggtgaagacg gagaacgcgc
    12601 gctcgagcgg cgcctcgacc accacgtcgt ggcggataga cgtcgcggat gccggtgtgc
    12661 tcatgagatc tcctgttcag tttgttcgac ggccgccttg taggcggtca gagcggtgcg
    12721 ccagaaccgg tccaagtcgg cgcgcagcgc gactaggccg tccgggttca cgcgatagat
    12781 gcgtcggttg ccggcgggct cgtccacgac gagtccggcc tgcttcagga ccttgaggtg
    12841 ctgcgacacg gccggccggc tcactggcaa ccctccggcg atctcgccga ccgctgacgg
    12901 gctctccgct agccgctcga agatcgcgcg cctggtgcga tcaccgagcg ccgaccacgt
    12961 gtcaggttgg taagtctgca ctcaccgtaa gcatacactt acgtagttct ttccgcgtcg
    13021 tcaacagctc acggccccgt ccacccccag gcgtagcagg ggtggtcgcc gcagacgttc
    13081 accacggcgc cgtccggctt caggaactca gacaccaggg gccgagccgg cctcgcgccg
    13141 gtagcagcta ggtcaggcgg tggagcgacg tctcggagat cttccgcccg agcgcctcca
    13201 cctcttcgtc ggtcatgtcg gcggtgtcga agcccgcacc ctcgagctcc tcgcgcacct
    13261 ttacgaccgc ggagatgaga tcgatcgacg cgccaagctt catcgtcgcc gccttgacca
    13321 tgctgtcgtc gagcgccgtc ttgccgtcgt tcgtcgccgt cagcagccag gcgcggagcg
    13381 cctccgcttc gggcacgctc agctcgagcg cgaccgttcg cttctggggc gcctcgacgc
    13441 tgggggtgaa gccgttgccc tgctcgatcg tggccatttg tttcgggccc tccgtgtatg
    13501 gccgcgcccg gtgcgcggct tcccttgcgg ctctatcgga ccgcaatcgc gcattcttga
    13561 gccattccgc ggcgatctgg ccacactcga cagcgcggcg acggttgcgc tatcgcgcga
    13621 tgacgggcag ctccatcccg cgcgacgcgg cccactcgcc aatgtccgcg gcgaccgcgc
    13681 cccagtccac gcccgcggcc aggtaatcgg cctccgcgtg cgtggcgaga gcgggcacgg
    13741 gcgcgaacag cctgtgcggg gacgcgagcc tcggaagcgc cccggcggtg aggcgcagcc
    13801 gccaggcgag caccttcgcg cggcgctcga gcggttggcc gtccatgccc tggaagagat
    13861 cgcgcggccg gtaggggagg tgaccaccga cggtcagcat gaccgcgtgg tcccaccagt
    13921 tgccgctgcg cggcgcgacc gcgtggagcc gctcgtcggc gcggaaggtc gtcacgcgcg
    13981 cagctacgct ggcggtcgtg ctcgtggcct ctcgccaggt cacgtccgcg gcctcgacgg
    14041 actc
//
LOCUS       DASVXS010000052         6180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_997831, whole genome shotgun sequence.
ACCESSION   DASVXS010000052 DASVXS010000000
VERSION     DASVXS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997831"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF752_02850"
     CDS             <1..442
                     /locus_tag="VF752_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX8854511.1"
                     /translation="ADGWDAAIAGPGPGILGSATALGHGGLVALDNAHAALALGCRTL
                     LIPRMSSGDPRPRHRWLSHHTSTVLRLLLRPVTVAVPEGEAAGVRRAVGEKHRVVEVS
                     VDLDDYAGSGLPARTMGRDIADDELFFKAALAGGTALAKEIHGA"
     gene            432..962
                     /locus_tag="VF752_02855"
     CDS             432..962
                     /locus_tag="VF752_02855"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8854512.1"
                     /translation="MGLERIGAEDVWQGKIASVRIERFRDEKGEEYSREVVSHPGAVG
                     VVAHDGERLYLVRQPREAVGEEALLELPAGKLDEEGEDALQTAKRELAEEIGKGANTW
                     QHLTTFYSSPGFSDEEVHVYLATDLYDERAEPAEDERIEIETVPLSDLDRLIRENRDA
                     KTLVGLLWFKAYIQQA"
     gene            959..2323
                     /locus_tag="VF752_02860"
     CDS             959..2323
                     /locus_tag="VF752_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8854513.1"
                     /translation="MRGTLAIVAASVAALAAAPAAYAAPRHVTNGSDDGPGSLRQALA
                     DALDDDTIVFDNAGENVSVTSGPLVVDHRVTITDTRGATVTGSGPNPVIEFASGSSNS
                     GLFGVGVRRAGGTGVRVDSGVTGVKIQRATIFGAGITPIDLAAGANGDVAAPALRVGP
                     RQPDGSLPVNGSAAPGTVDLYLGDPATNAGTSFMAQVGSDGSYSYTPSPELAPGQKVA
                     ATITGATGTSEYAVATTPSDLVSPTILSARGTSQSEIYIQPSEPIDPASVAGGDFSLS
                     MGGKPRPISGGAMAPDGSWIRLNSSQPWLAGEAGTLTLGGPGAVQDMAGNWNLAPKTV
                     FVSASPGDFTPPFVTSLSVKPRTICLTKARRCKKPGAKVSFVSSEPGRLSLIVTRGTK
                     RVGVRNFTIEKAGKNQVNFNGVVRSKKLRAGTYLMEVSMADAVGNDTADPPFATFKVK
                     RTTR"
     gene            2377..3321
                     /gene="xerD"
                     /locus_tag="VF752_02865"
     CDS             2377..3321
                     /gene="xerD"
                     /locus_tag="VF752_02865"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX8854514.1"
                     /translation="MAVAGTDLRTQPFEHLMLDFLAYLEFERGLSRNTLEAYRSDLLQ
                     YGRFLESRGVEAAGASTRDVSDFLQALANGDATGGAGASPATVHRKAACLRSFYRHLR
                     RENVLETDPTASLTAPRRGQKLPNVLTRGEVASLLEQPKGTDPTSLRDRALLELMYAC
                     GLRASEAIGLEVRDVDLETEMLRARGKGSKERVVPIGRKAVESLRIYMERGRPKLVNG
                     TPEPHLFVNFRGGQLTRQGLYKIVSRHAVSAGLGDRMSPHTLRHTFATHLLAGGCDLR
                     SVQEMLGHADVSTTQVYTHLSSERLKDVYFKAHPRATT"
     gene            3346..4509
                     /locus_tag="VF752_02870"
     CDS             3346..4509
                     /locus_tag="VF752_02870"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX8854515.1"
                     /translation="MIGRRRAFVVVLDACGCGALPDAADYGDEGTNTLAHLAEASGGL
                     RLPALEHLGLGNILPLRGVAPVERPATHGRLHHIGPGKDSTAGHWELMGVSAPRAPVY
                     PEGFPPELIERFERATGRGVIGNAPREGLRAIEEFGEEHLSTGKLIVYTSQDSVFQVA
                     AHVDVVPEPELYKHCERARELLIGEHAVGRVIARPFEGEPGGFRRTDGRRDLSLTPPG
                     PTYLDAVRDAGLPVHSVGKAGDMFAGQGIDHHHPAPNNPSAIAVISRLVGELDSGLVF
                     ANLIDTDQVYGHRKDVEGFHRSLQATDAAVESWLAGMRGGDMLVLTADHGCDPLHPGT
                     DHTREHAPLLATFAGCDGRRVDGPLADVGASALVWLTGRRSERLPGEPFVELR"
     gene            4559..4855
                     /locus_tag="VF752_02875"
     CDS             4559..4855
                     /locus_tag="VF752_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854516.1"
                     /translation="MHARIGRITYSPEKADDLKKFVQETTVPRYEGADGYKGFTLLLD
                     SSSGQAIGISFWESEEAMRASDDLGDQARQGASQAASGSDQGKQYFEVAVDTMA"
     gene            complement(4868..5245)
                     /locus_tag="VF752_02880"
     CDS             complement(4868..5245)
                     /locus_tag="VF752_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854517.1"
                     /translation="MLASAALVAFVPAADLDRSDDFYGGVLGLERVESSGFANVYDAN
                     GTMLRVTRVENPMRTPYTVLGWTVKDIAAAARDLAAQGVWLKRFQGMDQDAAGIWTAP
                     GGSRIAWFEDPDGNILSLQQPPG"
     gene            5284..6144
                     /gene="mutM"
                     /locus_tag="VF752_02885"
     CDS             5284..6144
                     /gene="mutM"
                     /locus_tag="VF752_02885"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX8854518.1"
                     /translation="MPELPEVETIRRQLAPRVEGRVLERLEVLDPRWCEPAPPEALAD
                     AVRGRRVERLGRRGKYLIVSLEDEVHLVMHLRMTGNLFVVSEDDDLPDRPHLRARLVL
                     DSGDRVLFVDVRRFGTGIVLLGDDALEDYFSARLGVEPLGDDFTPDALRAQAKGRKAP
                     VKAFLLSQERVAGVGNIYADEALWLAKIHPLRPVGTLTRAQIEALRDAVVASLEAGIE
                     AKGASFDDFRHVDGAYGSFQDRFLVHQREGEPCPRCGSTIKKMRAAGRGTYVCETCQP
                     KPRPRRKVPA"
BASE COUNT          972 a         2133 c         2211 g          864 t
ORIGIN      
        1 ggccgacgga tgggacgcag ccatcgccgg ccctggcccc ggcatcctgg gctcggcaac
       61 ggcactcggc cacggcgggc ttgtcgcgct cgacaacgct cacgcggcgc tcgctctcgg
      121 ctgccgcacg ctcctcatcc cgcgcatgtc gtcgggggac ccgcggccgc gccaccgctg
      181 gctctcacac cacacgagca cggtcctgcg gctgttgctt cgcccggtca cggtcgcggt
      241 ccccgagggc gaggccgccg gggtccggcg cgccgtagga gagaagcacc gtgtcgtcga
      301 agtgagcgtc gacctcgatg actatgctgg ctccggcctg ccagcccgga ccatggggcg
      361 ggatatcgca gacgacgagc tgttcttcaa ggcggcgctg gcgggcggca ccgcactcgc
      421 gaaggagatc catggggctt gagcgcatag gcgcggagga cgtctggcag ggcaagatcg
      481 cgagcgtccg gatcgagcgc ttccgtgacg agaagggtga ggagtacagc cgcgaggtcg
      541 tgtcacaccc gggcgcggtc ggggtcgtgg cccacgacgg ggagcgcctc tacctggtgc
      601 gccagccgcg cgaggccgta ggcgaggaag cgctgctcga actgccggcc gggaagctcg
      661 acgaggaggg cgaagacgcg cttcagacag ccaagcgcga gctcgccgag gagatcggca
      721 agggcgccaa cacgtggcag cacctcacaa ctttctactc gtcgccgggt ttctccgacg
      781 aggaagttca cgtgtacttg gccacagacc tctacgacga gcgggcggag cccgccgagg
      841 acgagcgcat cgagatcgag acggtgccgc tgtcggacct cgatcgcctg atccgcgaga
      901 accgcgacgc caagacgctc gtggggctgc tctggttcaa ggcgtacatc cagcaggcat
      961 gaggggaacg ctcgcgatcg tcgcggcgtc ggtcgccgcg ctggctgccg cgcccgcggc
     1021 atacgcggcc ccccgacatg taacgaacgg gagcgacgac gggcccggtt ctctccgcca
     1081 ggcgctcgcc gatgcgctcg acgacgacac gatcgttttc gacaacgccg gcgagaacgt
     1141 gtcggttacg agcggaccgc tcgtggtgga tcaccgcgtc acgatcaccg atacgcgcgg
     1201 tgccacggtg accgggtccg ggcccaatcc ggtgatcgag ttcgcgtccg gctcgagcaa
     1261 ctccggactc ttcggcgtag gcgtacgccg ggcgggcggc acgggagtcc gtgtcgacag
     1321 cggggtcacc ggtgtgaaga tccagcgggc cacgatcttc ggggccggaa tcacgccgat
     1381 cgacctggcg gcgggcgcca acggcgacgt agccgcgccc gccctgcgcg tagggccccg
     1441 ccaacccgac gggagtctcc ccgtaaacgg gagcgcggcg ccggggacgg tcgacctcta
     1501 cctcggtgac ccggcaacca acgccggaac gagcttcatg gcccaggtcg gctccgacgg
     1561 ctcgtacagc tacacgccgt cgcccgagct cgcacccgga cagaaggtgg ccgccacgat
     1621 caccggtgcc accggcacgt cggagtacgc ggtggcgacg acaccgagcg acctcgtctc
     1681 gcccacgatc ctgagtgcgc gcggcacctc gcagagcgag atctacatcc agccgagcga
     1741 gccgatcgac ccggccagcg tcgcgggcgg cgacttctcg ctctcgatgg gcggtaagcc
     1801 gcggccgatc tcgggcggcg cgatggcacc cgacggctcg tggatccggc tcaactcgag
     1861 ccagccctgg ctcgccggcg aggcgggcac gctcacgcta ggtggcccgg gggcggtgca
     1921 ggacatggcc ggcaactgga acctggcgcc gaagaccgtt ttcgtgtcgg catctccggg
     1981 cgacttcacg ccgccgttcg tcacgagcct ctcggtcaag ccgcgcacta tctgcctgac
     2041 gaaggcgcgc cgctgcaaga agccgggcgc caaggtgagc ttcgtctcga gcgagcccgg
     2101 ccggctcagc ctgatcgtga cgcgggggac gaagcgcgtc ggcgtgcgca acttcacgat
     2161 cgagaaggcg ggcaagaacc aggtgaactt caacggggtc gtgcgctcga agaagttgcg
     2221 cgccggcacg tacctgatgg aggtctcgat ggccgacgcg gttggcaacg acacggcgga
     2281 cccgccgttc gcgaccttca aggtcaagcg caccacgcgc tgagcgagcc gttgcagcgg
     2341 gaggggaagc ccggcagcta ggcgaaaggt ccgggcatgg cggtcgccgg cacggaccta
     2401 cgcacgcagc ccttcgagca cctgatgctc gacttcctcg cgtacctcga gttcgagcgg
     2461 gggctgtcgc gcaacacgct cgaggcctac cgctctgacc tgctgcagta cgggcgcttc
     2521 ctcgaaagcc gcggcgtgga agccgcgggc gcgagcacgc gcgacgtgtc ggacttcctg
     2581 caggcgctcg cgaacggaga cgcgacgggt ggagccggcg cctcgcccgc gaccgtgcac
     2641 cgcaaggccg cgtgcctgcg ttcgttctat cgccacctgc ggcgcgagaa cgtgctcgag
     2701 acagatccga cagcgagcct caccgcaccc cggcgcgggc agaagctgcc gaacgtgctg
     2761 acgcgcggcg aggtcgcgag cctgctcgaa cagccgaagg ggaccgatcc cacctcgctg
     2821 cgcgaccgcg cgctgctcga actgatgtac gcctgcgggc tgcgcgcgtc cgaggcgatc
     2881 gggctcgagg tgcgcgacgt cgacctcgaa accgagatgc tgcgcgcccg cggcaagggc
     2941 tcgaaggagc gcgtggtgcc gatcgggcga aaggccgtcg agtcgctgcg gatctacatg
     3001 gagcgcggtc gccccaagct cgtgaacggc acgccggagc cccacctgtt cgtgaacttc
     3061 cgcggcggcc agctcacccg ccaggggctc tacaagatcg tgagccggca cgcggtcagc
     3121 gccgggctgg gcgaccgcat gagcccgcac acgctccgcc acacgttcgc gacgcacctg
     3181 ctcgccggcg gctgcgacct gcgctcggtg caggagatgc tcggtcacgc ggacgtaagc
     3241 accacccagg tgtacacgca cctctcgagc gagcggctga aggacgtcta cttcaaggcc
     3301 cacccgcggg ccacgacctg aacccgggct gctagaacgc ggctcgtgat tggtcggagg
     3361 agggcgttcg tcgtcgtcct cgatgcatgc ggctgcgggg ccctgcccga tgccgcggat
     3421 tacggcgatg aggggacgaa tacgttggcg cacctcgccg aggcgtccgg tggcctccgc
     3481 ctgcccgcgc tcgagcacct cgggctcggg aacatcctgc cgctgcgagg tgtcgcgccc
     3541 gtcgagcggc cggccacgca cgggcgcctc caccacatcg ggccgggcaa ggactcgacg
     3601 gctggtcatt gggagctgat gggcgtgtcc gcgcctcgcg cgccggtcta ccccgagggc
     3661 ttcccgcccg agctgatcga gcgcttcgag cgggcgaccg gccgcggcgt gatcggcaac
     3721 gcgccgcgag agggtctgcg cgcgatcgag gagttcggcg aggagcatct cagcacgggc
     3781 aagctgatcg tctacacctc gcaggactcg gtattccagg tggccgcgca cgtggacgtc
     3841 gtgccggagc cggagctcta caagcactgc gagcgcgcgc gcgagctgct cattggcgaa
     3901 catgcggtgg gacgagtgat cgcgcgcccg ttcgagggtg agcccggtgg gttccggcgt
     3961 acggatgggc ggcgcgacct atcactgacg ccgcccggtc ccacgtacct cgacgcggtg
     4021 cgtgacgcgg gcctcccggt gcattctgtg ggcaaggcgg gcgacatgtt cgccgggcaa
     4081 gggatcgacc atcaccatcc cgcgccgaac aacccttcgg cgatcgccgt catctcgcgg
     4141 ctggtgggcg agctcgactc ggggctcgtg ttcgccaacc tgatcgacac cgaccaggtc
     4201 tatgggcacc gcaaggatgt cgagggcttc catcgctcgc tgcaggcaac cgacgcggcg
     4261 gttgagtcgt ggctcgcggg gatgcgcggg ggcgacatgc tcgtgctgac ggccgatcac
     4321 ggctgcgacc cgctccaccc gggtaccgac cacacccgcg agcacgcgcc gctgctcgcc
     4381 acgttcgccg gctgcgacgg caggcgcgtg gacgggccgc tcgccgacgt cggcgcgagc
     4441 gcgctcgtgt ggctgacagg gcggcgctcc gagcggttgc cgggcgagcc cttcgtggag
     4501 ttgcggtgac cggggagcgg agttagcgtc gaagtgtcga ccgacggagg aggctccagt
     4561 gcacgcgcgt attggccgca tcacgtactc gcccgagaag gccgatgacc tgaagaagtt
     4621 tgtccaggag accacggttc cgcggtacga gggagccgac ggctacaagg gattcacgct
     4681 gctgctcgac agctcgagcg gccaggccat cgggatctcg ttctgggaga gcgaggaggc
     4741 gatgcgtgcg agcgacgacc tcggcgacca ggcccggcaa ggggcgtccc aggccgcgtc
     4801 gggaagcgat cagggcaagc agtacttcga ggtcgccgtc gacacgatgg cctgagcggt
     4861 tcgccgccta tccgggcggc tgctgaagcg acaggatgtt gccgtccggg tcctcgaacc
     4921 acgcgatccg gctgccgccc ggcgccgtcc agatgccggc tgcgtcctgg tccatgcctt
     4981 gaaagcgttt gagccacacg ccctgcgcag ctaagtcgcg cgcggcggcc gcgatgtcct
     5041 tcaccgtcca gccgagcacc gtgtacggag tacgcatcgg gttctcgacg cgcgtcacgc
     5101 gcaacatcgt gccgttcgcg tcgtagacgt tcgcgaaccc gctcgactcg acacgctcca
     5161 acccgagcac gccgccgtag aagtcgtcgg accggtcgag gtccgccgcc gggacgaacg
     5221 ccacgagcgc tgcggaggcg agcactgggt gagcctatcg acgccccgac tagcctcaat
     5281 tccatgcccg agcttcccga ggtagagacg atccgccgcc agctcgcgcc ccgcgtggag
     5341 gggcgcgtgc tcgagcgcct cgaggtgctc gacccgcgct ggtgcgagcc ggcgccgccc
     5401 gaggcactcg ccgacgccgt gcgtgggcgg cgggtggagc ggctcggtcg ccgcggcaag
     5461 tacctgatcg tgtcactcga ggacgaggtc cacctcgtaa tgcacctgcg catgacgggc
     5521 aacctcttcg tcgtctcaga ggacgacgac ctgccggatc gcccgcacct gcgcgcccgg
     5581 ctcgtgctcg actcgggtga ccgcgtcctg ttcgtggacg tgcgccgctt cggcaccggc
     5641 atcgtgctgc tcggcgacga cgcgctcgaa gactacttct cggctcgcct cggcgtcgag
     5701 ccgctgggcg acgactttac gcccgacgcg ctgcgcgccc aggcgaaggg gcggaaggcg
     5761 ccggtgaagg cgttccttct gtcccaggag cgggtggccg gcgtcggcaa catctacgcg
     5821 gacgaggcgc tctggctggc gaagatccac ccgctgaggc cggtcgggac gctcacgcgc
     5881 gcacagatcg aggcgctgcg cgacgccgtc gtggcgtccc tcgaggcggg catcgaggcg
     5941 aagggagcga gcttcgacga cttccgccac gtggacggcg cctacggcag cttccaggat
     6001 cgcttcctcg tgcaccagcg cgagggcgag ccatgcccgc gctgtggcag cacgatcaag
     6061 aagatgcgtg ccgccgggcg ggggacctac gtctgcgaga cgtgtcagcc caagccgcgg
     6121 ccgcgccgca aggtgcccgc ctagtcgcta ggccgccatc aggcggtcga gcacgccgtc
//
LOCUS       DASVXS010000053         5015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1357595, whole genome shotgun sequence.
ACCESSION   DASVXS010000053 DASVXS010000000
VERSION     DASVXS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357595"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF752_02890"
     CDS             complement(<1..261)
                     /locus_tag="VF752_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854519.1"
                     /translation="MSESAYARAGVDTSRSDAAVAGLVEVLRSIDPGRPTRSMVGSGH
                     YAAVLRLDERTGIALCTDGVGSKVILAEQLGRFDTVGIDCIAM"
     gene            complement(258..1028)
                     /locus_tag="VF752_02895"
     CDS             complement(258..1028)
                     /locus_tag="VF752_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8854520.1"
                     /translation="MPALSVRQALVLGALHGPAELLPVSSSAHVALIPQLLGWPYTSL
                     EPSERKSFEVAAHSGAALGLAWVARRDLGTLRSAILTTLPPALAGFALERPVEERFGS
                     PRQLALTQVFAGVLLAAADHRGATRPERDAGALDELAIGAAQAVALIPGVSRMGAVLT
                     MARALGFDRAASARLARRAALPVLAGAAALKGARGVPRRLAAPFAAGAATSLVSTLAC
                     VPLVRRLDRSRSLAPFAAYRVALGAAILAAGRSGSMAA"
     gene            complement(1032..1664)
                     /locus_tag="VF752_02900"
     CDS             complement(1032..1664)
                     /locus_tag="VF752_02900"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8854521.1"
                     /translation="MLILASITDQLVTFATNVVGDLGLGGIALLMVPESACIPIPSEA
                     TMLFAGFNVSEGKYSLLAAVAVGVAANVVGSWIAYAIGYYGRIDLLEKHGRALHVKRE
                     HLDWADRWFERYGEATVFFSRMLPIVRTFISLPAGVARMPFWRFTLFTLLGCVPWVFL
                     LAFVGKQAGQNWEDWKNSLHYVDYAVAGLIVLGIVYLVFRRRRRATAETA"
     gene            1693..2775
                     /locus_tag="VF752_02905"
     CDS             1693..2775
                     /locus_tag="VF752_02905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8854522.1"
                     /translation="MPVSLFATSLDAYQDSIAERLAAVAADGRYILGPEVASFEREFA
                     GYLGAEHCVGVANGTDALTIALRALGLQSGDEVVVPSFTFYASAEAIVNAGGVPVFCD
                     IDPQSFNVTRETVERAVTPRTRAIMAVHLFGNVAPVPELLELGLPVVEDAAQAAGATL
                     DGRHAGALGTVATFSFFPSKNLPCLGDGGAIVTNDAEVAERARLLRFHGSKDKVTYED
                     VGYNSRLDSLQAGVLRLLLPELDGWIESRRAGAHAYEEEGLGQYVELPRPAPGCEHAY
                     HLYVVRSERADELLETLTERGIGARAYYRRPVHLQPATARFGGTSLDLPGTAEAARTH
                     LALPMGTQLSREHVAEVVTACAATAL"
     gene            2924..4780
                     /locus_tag="VF752_02910"
     CDS             2924..4780
                     /locus_tag="VF752_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX8854523.1"
                     /translation="MIDACAVGVAFYLAFVLRFDAGIPHRYDELLRDTIWWVIPFKVL
                     VFALFGLYHKLWRFVDQKDFESTVRAVVVADLLLVAILFAISPGGTDPPRGVIALDLL
                     LTLVLVGGTRFLVRSLVERPFRAPLVRDMREVLVVGAGNGGQSVVDELRRNPELRQLP
                     IGFVDDDPRKQGMRVRGLKVLGSTNDLKRVLDDAEPDEVIIAIPSAPGVVRQKVVTAC
                     RERGIPVRTLPTVFEILSGGINLTRQLREVQVEDVLGREPVRVEIDRVGSYLAGEVVL
                     VTGAGGSIGAELCRQIARVGPARLVLVEHGENALFEIRRELEDDRHFTRATAVLADCK
                     DAVRMREVFLEHKPSFVFHAAAYKHVGLMEENPIEAVRNNAVATRIVAAAAGDAGARR
                     FVLVSTDKAVTPATVMGASKALAEWAVEAAQSRHAGTGYTIVRFGNVLGSSGSVVPIF
                     RRQIAAGGPVTVTDPAMTRYFMTIPEAVQLIIRSGPLGDGGEIYVLEMGDPVKIMDLA
                     ENMIRLSGQEPGVDIAISIVGRRPGEKLHEELFNPDERARPTPAEKIVAAVRERLDPV
                     WVDDAFSQVEELSYQGDAAGLAKTVAKLSSERMQAATAALEQPAGPESPTRI"
     gene            4813..>5015
                     /locus_tag="VF752_02915"
     CDS             4813..>5015
                     /locus_tag="VF752_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854524.1"
                     /translation="MTIVQEIGAYAGFAAVVGLAVLAALYFSQARDVKRLREWAGRAP
                     ERAAEQEARIRAAGQAAAAQPAKA"
BASE COUNT          751 a         1759 c         1798 g          707 t
ORIGIN      
        1 catggcgatg cagtcgatgc cgacggtgtc gaagcgcccg agctgctcgg cgaggatcac
       61 cttcgagccg accccgtcgg tgcagagcgc gatgccggtg cgctcgtcga gcctgaggac
      121 ggcggcgtag tgacccgagc cgaccatcga ccgagtgggt cgcccgggat cgatcgagcg
      181 cagcacctcc acgaggccgg ccaccgcggc gtcggacctc gaggtgtcga cgccggcgcg
      241 tgcgtaggcg ctctcgctca tgcggccatt gaacctgatc gcccggccgc gaggatcgct
      301 gcgcccagcg cgacgcggta ggcggcgaag ggcgcgagcg agcggctgcg atcgaggcgt
      361 cgcacgagcg gcacgcaggc gagcgtggat acgagcgatg tggcggcccc cgcggcgaac
      421 ggcgccgcga gccggcgcgg cactccgcgt gcgcccttca acgcggccgc cccggccagc
      481 acgggcagcg ctgcgcgtcg cgcgagccgc gccgacgccg cgcggtcgaa gcccagggca
      541 cgcgccatcg tgagcacagc gcccatgcgc gaaacgccgg ggatgagggc caccgcctgg
      601 gccgccccga tcgccagctc gtcgagcgct ccggcgtcgc gctccgggcg ggtcgcgccc
      661 cggtggtctg ccgcggcgag caggacgccg gcgaacacct gcgtgagcgc cagctgccgc
      721 ggcgagccga agcgctcctc gaccggccgc tcgagcgcaa agcccgcgag cgcaggcggc
      781 agcgtcgtga gaatcgcgga gcgcagcgtg ccgaggtctc gccgtgcgac ccaagcgagc
      841 ccgagtgccg cccccgaatg cgccgctacc tcgaacgact tgcgctcact cggctcgagc
      901 gaggtgtacg gccagccgag tagctgcggg atgagcgcca catgcgcgct gctcgacacc
      961 ggcagcagct ccgcgggccc gtgcagcgcg ccgagcacga gcgcctgccg gacgctcagg
     1021 gcgggcaacg gctaggccgt ctcggccgtg gcgcgccggc ggcgcctgaa caccaggtac
     1081 acgattccga gcacgatcag tcctgccacc gcgtagtcca cgtaatgcag ggagttcttc
     1141 cagtcctccc agttctggcc ggcctgcttg cccacgaacg cgagcaggaa gacccacgga
     1201 acgcaaccga gcagcgtgaa cagcgtgaag cgccagaacg gcatccgggc gacgcccgcg
     1261 ggcagcgaga tgaacgtgcg cacgatcggc agcatgcgcg agaagaacac ggtcgcctcg
     1321 ccgtagcgtt cgaaccagcg gtccgcccaa tcgaggtgct ctcgcttgac gtgcagcgcg
     1381 cgcccgtgct tctcgagcag gtcgatgcgg ccgtagtagc cgatcgcgta ggcgatccac
     1441 gatcccacca cgttcgcggc gacgcccacg gcgacggcgg cgagcagcga gtacttgccc
     1501 tccgacacgt tgaagccggc gaagagcatg gtggcttcgc tcggaatcgg gatgcaggcg
     1561 ctctccggaa ccatgagaag cgcgatgccg ccgagcccga ggtcgcccac gacgttggtc
     1621 gcaaaggtga ccaactgatc ggtgatcgag gcgaggatga gcattcggga gagcgaagta
     1681 gggtagcgag ccgtgccggt gtcactgttc gcaacctcgc tcgacgccta ccaggacagc
     1741 atcgcggagc gcctggcggc ggtcgcggcg gatggacgct acatcctcgg gccggaagtc
     1801 gcctcattcg agcgcgagtt cgccggctac ctgggcgccg agcactgcgt cggcgtggcc
     1861 aacggcacgg acgcgctcac gatcgcgctg cgcgcgctcg gcctccagtc gggcgacgag
     1921 gtcgtcgtgc cgtcgttcac cttctacgcg agcgccgagg cgatcgtgaa cgcaggcggc
     1981 gtgccggtgt tctgcgacat cgaccctcaa tcgttcaacg tcacgcgtga gaccgtcgag
     2041 cgcgcggtca ctccacgcac gcgcgcgatc atggcggtgc acctgttcgg caacgtcgcg
     2101 ccggtgcccg agttgctcga gctcgggctg ccagtcgtcg aggatgcggc gcaagctgcc
     2161 ggtgccacgc tcgacggccg ccacgcggga gcgctcggca cggtcgccac gttctccttc
     2221 ttcccgagca agaatctgcc ctgcctcggc gacggcggcg cgatcgtcac gaacgacgcg
     2281 gaggtggccg agcgggcgcg gctgttgcgt ttccacggct cgaaggacaa ggtcacctac
     2341 gaggacgtcg gctacaactc gcggctcgac tcgctgcagg ccggggtgct gcggctcttg
     2401 ctgcccgagc tcgacggctg gatcgagtcg cggcgggcgg gggcgcatgc gtacgaggag
     2461 gagggcctgg gtcagtacgt ggagctgccg cggccggcgc ccggctgcga gcacgcctac
     2521 cacctgtacg tggtgcgctc cgagcgcgcg gacgagctgc tcgagacgct caccgagcgg
     2581 ggcataggcg cgcgcgcgta ctaccgccgc ccggtgcatc ttcagcccgc tactgcgcgc
     2641 ttcggcggga cctcgctcga cctgccgggg accgcggagg cggcccgcac gcacctggcg
     2701 cttccgatgg gcacgcagct gtcccgcgag cacgttgcgg aggtggtcac ggcatgcgct
     2761 gcgaccgccc tctgacggcg ctaccatctc gcactggcgt cccgcgcgga tgctcggctc
     2821 gcgtgcgccc gctggcccat tccacgacag tcccagccgg ttcttgcact ctctgaggac
     2881 acgcctctcg acatccgtcg ccgcccgcag gatcgggcag gtcgtgatcg acgcctgcgc
     2941 cgtcggcgtc gcgttctacc tcgcgttcgt gctgcgcttc gacgcgggca ttccgcaccg
     3001 ctacgacgag ctgctgcgcg acacgatctg gtgggtcatc cccttcaagg tgctcgtatt
     3061 cgcgctgttc gggctctacc acaagctctg gcgcttcgta gaccagaagg acttcgagtc
     3121 gacggtgcgc gcggtcgtgg tcgcggacct gctgctcgtc gcgatcctct tcgccatctc
     3181 accgggcggg accgacccgc cacgcggcgt gatcgcactc gacctgctcc tcaccctcgt
     3241 gctcgtgggt ggcacgcggt tcctggtgcg ctcgctggtg gagcgcccgt tccgcgcccc
     3301 gctcgtgcgc gacatgcgtg aggtgctggt ggtcggcgcg ggcaacgggg gccagtcggt
     3361 cgtcgacgag ctccgccgca accccgagct gcgccagctg ccgatcggct tcgtggacga
     3421 cgacccgcgc aagcagggca tgcgcgtgcg cggcctgaag gtgctcggct cgaccaacga
     3481 cttgaagcgc gtcctcgacg acgccgagcc ggacgaggtg atcatcgcga tcccgtcggc
     3541 cccaggcgtc gtgcgccaga aggtggtgac cgcgtgccgc gagcgcggca tccccgtgcg
     3601 cacgctgccg accgtcttcg agatcctttc gggcggcatc aacctcacgc gccagctgcg
     3661 cgaggtgcag gtggaggacg ttctcggccg cgagcctgtg cgcgtggaga tcgaccgcgt
     3721 cggctcctac ctagccggcg aggtggtgct cgtcaccggc gcgggcggct cgatcggtgc
     3781 cgagctctgc cgtcagatcg cccgcgtggg ccccgcccgg ctggtgctcg tcgagcacgg
     3841 cgagaacgcg ctcttcgaga tccggcgcga gctcgaggac gaccgccact tcacgcgtgc
     3901 gaccgccgtg cttgccgact gcaaggacgc ggtgcggatg cgcgaggtgt tcctcgagca
     3961 caagccctcg ttcgtcttcc atgccgccgc ctacaagcac gtcggcctga tggaggagaa
     4021 cccgatcgag gccgtgcgca acaacgcggt ggccacgcgc atcgtggcgg cggccgcggg
     4081 tgacgccgga gcgaggcgct tcgtgctcgt ctcgaccgac aaggcggtca cgccggcgac
     4141 cgtgatgggc gcctcgaaag cgctcgcgga gtgggcagtg gaggcggcgc agagccgcca
     4201 cgcaggcacg ggctacacga tcgtgcgctt cggcaacgtg ctcggctcct cgggctcggt
     4261 ggtgccgatc ttccgccgcc agatcgcggc cggcggaccg gtgacggtca ccgacccggc
     4321 gatgacgcgc tacttcatga cgatcccgga ggcggtgcag ctcatcatcc gctccggccc
     4381 gctcggggac ggcggcgaga tctacgtgct cgagatgggc gatccggtga agatcatgga
     4441 ccttgccgag aacatgatcc ggctctcggg acaggagccg ggggtggaca tcgccatctc
     4501 gatcgtgggg cggcgaccgg gcgagaagct ccacgaggag ctgttcaacc ccgacgagcg
     4561 cgcgcgcccc acgcccgcgg agaagatcgt cgccgccgtg cgcgaacggc tcgatccggt
     4621 ctgggtggac gacgcgttct cgcaggtcga ggagctgagc tatcagggcg atgcggcagg
     4681 cctcgcgaag acggtcgcga agctctccag tgagcgcatg caggccgcca cagcagccct
     4741 ggaacaaccc gccggccccg agtcgccaac gcgcatctag tcacccgcgc gcacggttaa
     4801 ggttcacgac gaatgaccat cgtccaggag atcggcgcct acgcagggtt cgcggcggtc
     4861 gtgggactgg cagtgctggc cgctctgtac ttctcacagg cgcgcgatgt gaagcgcctg
     4921 cgcgagtggg ccggtcgtgc gccggaacga gcagccgagc aggaggcgcg gatccgcgcc
     4981 gcgggccagg ccgctgccgc ccagcccgcg aaggc
//
LOCUS       DASVXS010000054        15293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_572453, whole genome shotgun sequence.
ACCESSION   DASVXS010000054 DASVXS010000000
VERSION     DASVXS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15293
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572453"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VF752_02920"
     CDS             complement(<1..539)
                     /locus_tag="VF752_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854525.1"
                     /translation="MPLLERPEGVLIHWEERGEGPLVVLVTPMFSTHDVYEDLFDLLA
                     RDHRLVYYDVRGVGQSSRVGPYDIDTDAGDLAAVVEAAGPPAVVAASGESLHRAARVA
                     AARPDLIPLVLNVGGTPTGPQRTYGKSSGLAGSMTVIQAFVQLISSDYRGGLRSAIAP
                     ANTQLDDAGVRDRIEKLVE"
     gene            complement(517..996)
                     /locus_tag="VF752_02925"
     CDS             complement(517..996)
                     /locus_tag="VF752_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX8854526.1"
                     /translation="MSGVVERIFLAREESGPTAPVEEVEAVPGHGLVGDRYWYENGDA
                     GPGKAITLIAAEAIEGYAADTGNPLAPGEHRRQVVTRGIDVNALVGRRFFVGEVECEG
                     VELCEPCNHLQKLTGRDSLMRDLVHRAGLSADIVSGGTIRLGDSVEAVERDAASRTA"
     gene            complement(993..1457)
                     /locus_tag="VF752_02930"
     CDS             complement(993..1457)
                     /locus_tag="VF752_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8854527.1"
                     /translation="MAPVTAEGIEGLKDLIGQPVGPSEWREVTQEMIDQFAELSGDDQ
                     WIHVDVERAKTESPFGTTVAHGNLTLSLIDGFRKDLIQSNGFKLGVNYGWNKVRFPAP
                     VPSGSRVRASAEVVSVDGVGNGWWQIVTRFTIEVEGNEKPACVAESVGRALP"
     gene            complement(1488..2528)
                     /locus_tag="VF752_02935"
     CDS             complement(1488..2528)
                     /locus_tag="VF752_02935"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEX8854528.1"
                     /translation="MSSEQRLINLYSDTQTRPSREMREAMANAEVADEQRREDPTVNA
                     LQERVAYLLGHEAAVFLPSGTMCNAISFRLHIRPGGDEVVLDRTAHPIVAEAGGPASI
                     SGAMMNPLEGRNGIFSAEQVEAALRPPDRYLPRSRLVSIEQTTNMGGGRVWPLETIRE
                     VLDVARAHDMRAHLDGARLMNAVAAAGVSAAEYAGPFDTAWVDFSKGLGAPVGAVLCG
                     SHELIEEAWRYKQMWGGALRQAGVLAAACMYALDHNVDRLAEDHANAKRLAEGLAELP
                     GVELDPAAVETNIVIFEVPDAEAFVERAAAEGLQLSRTPGGGVRAVTHLDVSSEQIEQ
                     ALAIARGLVSPR"
     gene            complement(2545..2967)
                     /gene="ribH"
                     /locus_tag="VF752_02940"
     CDS             complement(2545..2967)
                     /gene="ribH"
                     /locus_tag="VF752_02940"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX8854529.1"
                     /translation="MADRWAIAVATFYEDLAEKLVEGASRVFDQAGGEVHVHEVPGAF
                     ELPLAAKYCAESRRYAGVACVGAVIRGETSHYDYVCSEAASGIARVSLDTGVPCSFGV
                     LTVENREQALERLGPKRHQGEDAARAVIRMVELHRELT"
     gene            complement(2960..4273)
                     /locus_tag="VF752_02945"
     CDS             complement(2960..4273)
                     /locus_tag="VF752_02945"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX8854530.1"
                     /translation="MSSPFSTIEEALEDIRAGRMVVVCDAEDRENEGDLTMAAQFVTP
                     EAINFMAKEGRGLICLALNPDRCDELGLDLMAAKNESPFQTAFTVSIEAREGVTTGIS
                     AHDRARTIQVAIDPRTRPHDLVQPGHIFPLKAKTGGVLERTGQTEAAVDLARLAGLNP
                     AGVICEIMNDDGTMARVPDLAAYCKRHGLKMITVADLIAYRRKHDKLIERVVETRLPT
                     DFGEFNVVGFRSLVDDKHHVAMVKGEIDGAEDVLVRVHSECLTGDVFHSQRCDCGQQL
                     EDALTRIEEEGQGVLLYLAQEGRGIGLLNKLKAYKLQEQGLDTVDANLELGLPADLRD
                     YGIGAQILVDLGLSTIRLLTNNPKKIVGLEGYGLEVTDQIPIAHAPTAHNRDYLRAKR
                     DRLGHMLHHQGLALDEEMIASERRLDRERAGAPTPERQEAEEPRG"
     gene            complement(4270..4857)
                     /locus_tag="VF752_02950"
     CDS             complement(4270..4857)
                     /locus_tag="VF752_02950"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8854531.1"
                     /translation="MFTGLVQGTGSVRAIERSEDGVRLEVATELASELGRGDSISVNG
                     VCLTAVDVADGGFRADVMAETLRRSSLGPLGEGDRVNLELALRAGDRLGGHMVQGHVD
                     GTGTVESVTDEGIAKLVRIACEPALLRYMVEKGSVAVDGVSLTIAAVDDDGFTVLLIP
                     ETIERTTLGLAGPGTVVNVEVDVLAKYVEKLAVRT"
     gene            complement(4857..5981)
                     /gene="ribD"
                     /locus_tag="VF752_02955"
     CDS             complement(4857..5981)
                     /gene="ribD"
                     /locus_tag="VF752_02955"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX8854532.1"
                     /translation="MTQLTATQRDERHLLRAIELADRARGMTSPNPLVGAVLVKDGRV
                     IGEGFHAAAGEPHAERMALASASEDPAGATMYVSLEPCCHHGRTPPCTDALIDARVGR
                     VVVASDDPTEKASGRGLGILRDEGITVDVAAGGAAQAARLLNQPFRKHAKTGHPLVVF
                     KSAMTLDGKVATHTGDSKWISSDASRARAHRWRAEMDAVAVGIGTALADNPLLTARVE
                     GVRRQPRRVVFDSEARLPAEGQLAASANEVPLTVVTSRAASRRAVAALEAAGAEVIVA
                     TGGNEAARVQSALSQLGELGVQSVLLEGGPHLAGAFMDAGEIDEARIFIAPMLLGGRR
                     ARVPLEGQGVETIEAADRALAIDSERIGDDLLITARMREW"
     gene            6039..7769
                     /locus_tag="VF752_02960"
     CDS             6039..7769
                     /locus_tag="VF752_02960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8854533.1"
                     /translation="MRRTRAAAWPAYAILALILVAGLYLRLRHNGYGLPYVYNQDEGR
                     HFTNRAVAMFGGGSWDPGYYENPSGFTYLLNIAMRFRYGHGWPFGGFDNVVRMFASPD
                     PSGVYELTRGVAALLCCAGAAGVFFVGRRLWGLREGLVAAAVLAFAFLPVAYSRIAVT
                     DVGTLLPVAIALFAAVRIYERGDLRWYLVGGAAVGFAIGFKYTAGLVLLPLLIATAFR
                     MRTDGLAPLKGLGIALVVATAAFFVTTPYFFINFSHARHQLTSQSQTVGDFGKVGQAQ
                     DNGFAYYLWSLTWGLGWVPLVAAAAGCFLVARRDPVRALLLAAFPLALFIYLSVQSRF
                     FGRWLLPVYPALALFAGYAIVQAVGAVAALRGRRLAAAGALAAVVLAALAQPLAADVR
                     TMRLLGRTDTRQLARDYLVKTYPPRLRIVIEPAVPGRYYRLARPGRKAPPERKQFVRG
                     FIRDIGETRIDYVTLLRPEVLDHYRRSGYCLVMTMSVIRGRAENDRLAPALAYYARLE
                     RESKLVFEASPYDKGVKHLKFNFDHSYDYYPSKFARPGPDVKIYRLNNCKQGYGPLPK
                     GAGVPQGVSE"
     gene            7766..9463
                     /locus_tag="VF752_02965"
     CDS             7766..9463
                     /locus_tag="VF752_02965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8854534.1"
                     /translation="MTAEAKPRTTARGATLLERVRRPTAAGWTLAGILVGALVLRLWS
                     IRHGMPWAFNYDEEHHFVPPAVSMLGGSLNPGYFENPPALTYLLFGVFKLRFHAGFPF
                     GASGFVRHFKNDPESAFVTARIVVALLGTLAVWLVHWAGSRYYGRRVGLLAAALVACA
                     FLPVFYSKQALNDVVTLVPLTVALGACLLVYERGRALDWALAGGAIGVATATKYTAGA
                     MVAVLAIAALLRLRDRRDDLGALVRGAVIAGVLFVLLFLLLNPYSLIDFSKFKSQVGG
                     QSATAGGLAKLGQDDVPGWLYYLWTLTWGFGWIPLAAAVGGAVVALRSDWRRGALLIV
                     FPIVLWLFLGGQARYFGRWFLPAYPALAILAAYGMVRLVDWFVARRGAVQAAPALLVA
                     AGFLLVVQGLAASVRVDSVLAHTDTRTLTREWLARNVPQGSGLVAEPFFPSGFFTTGG
                     RRAPDRYRLYPIKPPFQGYEKKLTPGLIDTYRSQRYCWVVTGTYQEGRGLKAGLANAK
                     AYYSRLDAESETVAVFSPWYAGEGPVEFNFDHSFDYYPRAYRRPGPLIEVHHLNGCR"
     gene            9454..9705
                     /locus_tag="VF752_02970"
     CDS             9454..9705
                     /locus_tag="VF752_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854535.1"
                     /translation="MPLDRRNIAKRVLLAPHFVRRAAASQAAAREEGWLLRQPKDVRA
                     SYVREVLDKGDGPRLAEIWMLRQRREVRESYIREVLGAD"
     gene            complement(9702..10745)
                     /locus_tag="VF752_02975"
     CDS             complement(9702..10745)
                     /locus_tag="VF752_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX8854536.1"
                     /translation="MARELKLGLNLGYWAGGPPPNAAETVKEAERLGYDSLWAAEAYG
                     SDVLTPLAWWGAQTERIRLGTAIVQMSARTPAATAMAAMTMDHLSGGRFILGLGVSGP
                     QVVEGWYGQAFAKPLARTREYISIIRQILARNGPVTFEGQHYRLPYEGGTGLAKPLKS
                     SIHPLREDIPIFLGAEGPKNVALAAELCDGWLALFFSPDHERFYRDALAEGFAREGAR
                     RSFDDFEIAASVNVVIDDDVERAADRMRPLYALYFGGMGAKKANFHADVARRMGYDAE
                     VDEIQELYLDGKKEEAAAAVPTELIERLALIGSPDKIRHDIEKWRDSMVTTLLIGADP
                     ALIRPMAELVLGA"
     gene            complement(10750..11214)
                     /locus_tag="VF752_02980"
     CDS             complement(10750..11214)
                     /locus_tag="VF752_02980"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX8854537.1"
                     /translation="MPERFSAKLTEADGGGGGRWLEVPFDPKQKFGQARAPVRGTLNG
                     TEFRGRIAVYGGRAVLGLNREIREAAGIELGDDVEVVLALDAQPREVEVPDALANALA
                     RADDARQAFDQLAFTHRKEYARWIAEAKRDDTRERRVKKAVEMLREGVKTPE"
     gene            complement(11320..12864)
                     /locus_tag="VF752_02985"
     CDS             complement(11320..12864)
                     /locus_tag="VF752_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854538.1"
                     /translation="MSKLITRGAPQPATVPPAAGLAGESRLATRAEALATRRNGARLL
                     AFVSAVSLGIFLFRPTYPNYDSYYSLVWGRALSHGHLPDYDVFRTPTPHPLWELLGAG
                     LSFFGGAADRLLVLITIASFIGLLVVLFRFAQHLLGTLIALVGVAVVCTRADLIFYAL
                     RGMVDVPFLLLVFGAALWELKSPRRGLPVLVLLGLAGLLRPEAWVLSGVYLLWLWPAI
                     SREQFIRYALLVAAAPLLWIASDWIVTGHPLHSLTSTRDVAGQFGRDRSVPEAVRLIP
                     DYVGANEKIVNVAAGGLGLLLALWMLRRRAYLPLALMAVGIGVFLMIAIAGLSVIPRY
                     LVIPSLLFNLGVGVALAGWAVITDRRARRVAIGVAALSLLLVMWRTPAWINDFRKLNG
                     QTLFVKKQHQGLKAILQDPSVTALMAKCRPITFPTHSALPVVEWETGLGKHDLQASIN
                     QTGPPTHGLLFLGSSFNFEPAAARSVTGVNQKSARKWWSNYPLSAFKFVAGNSMWRVY
                     ANCPGA"
     gene            13070..14143
                     /locus_tag="VF752_02990"
     CDS             13070..14143
                     /locus_tag="VF752_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8854539.1"
                     /translation="MKLIIQIPSWNEEETLPGTLADLPRQMPGVDVVEWLVIDDGSTD
                     RTVEVAREHGVDHIVRLTNNKGLAAAWQAGLDAALKLGADVIVNTDADNQYKGSEIPK
                     LVAPILAGDADMVIGDRQVDTIEHFSNLKVKLQQLGSAVVRRASGTHIEDTTSGFRAY
                     NREAALAMQVVSKFTYTLETIIQAGKMTVAVDHVPIGTNPKTRESRLFPSMWAYVRRN
                     AVSIFRIYALYEPLRVFMTAAIIVGIAAAAIWIRFLVAYIGGSGAGHVQSLILGAVLF
                     IAAVQLAALGVIGDVLAGSRVLSQRILERVRRVELQLGIEPSHYEPGKASTGQEATTG
                     ADAGPATGKPEEESTEEREAVKL"
     gene            14140..14760
                     /locus_tag="VF752_02995"
     CDS             14140..14760
                     /locus_tag="VF752_02995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854540.1"
                     /translation="MTQAPIEAVPTGNTFDKYGSTNPVVRRLMEGFQSTLDELWAKAA
                     PNSILDVGCGEGVLTYEWAERLGDRRIVGIDLDDDKLKAEWDTRQRANLEYVPADATS
                     LEFVDDEFDMASAIEVLEHVPEPERTLAEMARVARRHLLVSVPREPLWRGLNMARGAY
                     LKDFGNTPGHINHWSKRSFVSLLSRYGTVEEARSPFPWTMLLVRLS"
     gene            14744..>15293
                     /locus_tag="VF752_03000"
     CDS             14744..>15293
                     /locus_tag="VF752_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854541.1"
                     /translation="MSASASSPSATRSYGRGASILAVGIGVTGLVTYSYFALASHTLD
                     ATDYGQISLLWSAIFIVVSVLYRPVEQLLSRTISERDARGQHGNEHLRVAATIQLALG
                     LVFAVAALAFRDRLQNHLFGGSATLYWILIIAVLAYAASYFARGFLAGHRRFGLYGGL
                     VLMEATSRFLFALAVTVGIAHGQ"
BASE COUNT         2171 a         5472 c         5246 g         2404 t
ORIGIN      
        1 tactccacga gcttctcgat gcggtcgcgc acgccggcgt cgtcgagctg cgtgttcgcg
       61 ggcgcgatcg cgcttcggag cccgccgcgg tagtcgctcg agatcagctg cacgaacgcc
      121 tggatgaccg tcatcgaccc cgcgagcccg ctgctcttgc cgtaggtgcg ctgcgggccg
      181 gtgggcgtgc ccccgacgtt cagcacgagc gggatcaggt cgggtcgggc ggcggctacg
      241 cgcgccgctc ggtggagcga ctcgcccgat gccgcgacca ccgcgggcgg cccggccgcc
      301 tcgaccacgg cggcgagatc gcccgcgtcg gtgtcgatgt cgtaggggcc gacgcggctc
      361 gactgcccca ccccacgcac gtcgtagtag acgagccggt ggtcacgcgc gaggagatcg
      421 aagaggtcct cgtagacgtc atgcgtcgaa aacatcggcg tgacgagaac cacgagcggg
      481 ccctccccgc gctcctccca gtggatgagg acgccttcag gccgttcgag aagcggcatc
      541 ccgctcaacc gcctccaccg agtcgccgag gcggatcgtg ccgccactca cgatgtcggc
      601 gcttagccct gcccggtgca cgaggtcgcg catcaggctg tcgcgcccgg tgagcttctg
      661 caggtggttg cacggctcgc acagctcgac gccctcgcac tcgacctcgc cgacgaagaa
      721 gcggcgcccg accagcgcgt tcacatcgat cccgcgcgtg acgacctgac ggcggtgctc
      781 acccggggcg agcgggttgc cggtgtcagc ggcgtagccc tcgatcgcct cggcggcgat
      841 caacgtgatc gccttgccgg gcccggcatc gccgttctca taccagtagc ggtcacccac
      901 gagcccgtgg cctggaacgg cctcgacctc ctcgaccggc gccgttgggc cggactcttc
      961 ccgcgcgagg aagattcgtt ctacgacgcc gctcacggga gggcccgccc caccgactcg
     1021 gccacgcagg ccggcttctc gttgccctcg acctcgatcg tgaaacgggt gacgatctgc
     1081 caccagccgt tccccacgcc gtcgaccgac acgacctcgg cgctggcgcg cactcgcgag
     1141 ccggacggga cgggcgccgg gaagcgcacc ttgttccagc cgtagttcac gccgagcttg
     1201 aagccgttcg actggatcag gtccttcctg aagccgtcga tcaacgagag ggtgaggttg
     1261 ccgtgcgcga ccgtggtgcc gaacgggctc tcggtcttcg cgcgctccac gtcgacgtgg
     1321 atccactggt catcgccaga gagctcggcg aactggtcga tcatctcctg ggtgacctcg
     1381 cgccactcgc tcgggccgac cggctgtccg atgaggtcct taagtccctc gattccttct
     1441 gctgtaactg gggccatgcg ggtgagagta ccggtgggcc tgtccgatca ccgggggctg
     1501 acgagcccac gggcgatcgc gagcgcctgc tcgatctgct cggagctcac gtccaggtgc
     1561 gtgaccgcgc gtacgccgcc gcccggggtg cggctgagct ggagaccctc cgcggccgcg
     1621 cgctccacga acgcctccgc gtcgggcacc tcgaagatca cgatgttcgt ctccacggcc
     1681 gccgggtcga gctccacgcc gggcagctcc gcgagcccct cggcgagccg cttcgcgttc
     1741 gcgtggtcct cggcgagccg gtcgacgttg tggtcgagcg cgtacatgca ggcggccgcc
     1801 agcacgccag cctgacgcag tgcgccgccc cacatctgct tgtagcgcca cgcctcctcg
     1861 atcagctcgt gcgagccgca gagcaccgcc ccgaccggcg cgccgagccc cttcgagaag
     1921 tcgacccagg cggtgtcgaa cggcccggcg tactcggcgg ccgacacgcc cgcggccgcg
     1981 accgcgttca tcagccgcgc gccgtcaagg tgcgcgcgca tgtcgtgcgc gcgggcgacg
     2041 tcgagcacct cacggatcgt ctcgagcggc catacgcggc cgccgcccat gttggtggtc
     2101 tgctcgatcg acacgagccg cgagcgtggc aggtagcgat ccggcggacg cagcgccgcc
     2161 tccacctgct cggcgctgaa gatgccgttg cggccttcga gcgggttcat catcgccccg
     2221 gagatcgacg ccgggccgcc ggcttcagcc acgatcggat gagcggtgcg gtcgagcacc
     2281 acctcgtcgc cgcccggccg gatgtgcagc cggaacgaga tcgcgttgca catcgtgccg
     2341 ctgggcagga acaccgcggc ctcgtgcccg agcaggtagg ccacgcgctc ctgcagcgcg
     2401 ttcacggtcg ggtcctcgcg ccgctgctcg tcggccacct cggcgttcgc catggcctcg
     2461 cgcatctcgc ggctcgggcg agtctgcgta tccgagtaga ggttgatcag acgctgctcg
     2521 gagctcacgt gatctccgag cagatcacgt gagctcgcgg tggagctcga ccatccggat
     2581 cacagcgcgc gccgcgtcct cgccctggtg acgcttcggc ccgagccgct cgagcgcctg
     2641 ctcacggttc tcgacggtga gcacgccgaa ggagcacggt acgcccgtgt cgagcgacac
     2701 gcgcgcgatc ccgctcgcag cctcgctgca gacgtagtcg taatggctcg tctcgccgcg
     2761 gatcacggcg ccgacgcacg ccacgcccgc gtagcggcgc gactcggcgc agtacttcgc
     2821 ggcgagcggc agctcgaacg ccccgggcac ctcgtgcacg tgcacctcgc cgccggcctg
     2881 gtcgaacacg cgcgaggcgc cctcgacgag cttctcggcc aggtcctcgt agaacgtcgc
     2941 gaccgcgatc gcccagcggt cagccacgcg gctcctcggc ctcctgccgc tccggcgtgg
     3001 gcgcgcccgc gcgctcgcgg tcgaggcgcc gctcggaggc gatcatctcc tcgtcgagcg
     3061 cgaggccctg gtggtggagc atgtggccca ggcggtcgcg cttcgcgcgc aggtagtcgc
     3121 ggttgtgcgc cgtgggcgcg tgcgcgatcg ggatctggtc ggtcacctcg agcccgtagc
     3181 cctcgaggcc cacgatcttc ttcgggttgt tggtgaggag acggatcgtc gagaggccca
     3241 ggtccacgag tatctgcgcg ccgatgccgt agtcacggag gtcggcaggc aggccgagct
     3301 cgaggttggc gtcgacggtg tcgaggccct gctcctgcag cttgtaggcc ttcagcttgt
     3361 tgaggaggcc gatgccgcgg ccctcctgtg cgaggtagag cagcacgccc tggccctcct
     3421 cctcgatgcg ggtgagcgcg tcctcgagct gctggccgca gtcgcagcgc tgcgagtgga
     3481 agacgtcgcc cgtgaggcac tccgagtgca cgcgcacgag cacgtcctcc gccccgtcga
     3541 tctcgccctt gaccatcgca acgtggtgct tgtcgtcgac gagcgagcgg aagccgacca
     3601 cgttgaactc gccgaagtcg gtcggcagcc gcgtttcgac cacacgctcg atgagcttgt
     3661 cgtgcttgcg gcggtacgcg atcaggtcgg cgaccgtgat catcttgagg ccgtgccgct
     3721 tgcagtacgc cgcgaggtcg ggcacgcggg ccatcgtgcc gtcgtcgttc atgatctcgc
     3781 agatcacgcc cgccgggttg agcccagcga ggcgcgcgag atcgaccgcc gcctcggtct
     3841 gacccgtgcg ctcgagcacg ccgccggtct tcgccttcag cgggaagatg tggccgggct
     3901 gcaccaggtc gtgcgggcgg gtgcgtgggt cgatcgccac ctggatcgtg cgcgcgcgat
     3961 cgtgggcgga gatcccggtg gtcacgccct cgcgcgcctc gatcgacacc gtgaacgcgg
     4021 tctggaacgg actctcgttc ttggccgcca tgagatcgag gccgagctcg tcgcagcggt
     4081 cggggttgag agcgaggcag atcagcccgc gcccctcctt ggccatgaag ttgatggcct
     4141 ccggcgtgac gaactgggcg gccatcgtga ggtcgccctc gttctcgcgg tcctcggcgt
     4201 cgcagacgac gaccatgcga ccggcacgga tgtcctcaag cgcttcctcg atcgtgctga
     4261 acggcgagct catgtgcgca ccgccagctt ctcaacgtat ttagcgagca cgtccacctc
     4321 cacgttcacg acggtacccg gcccggcaag tccgagcgtg gttcgctcga tcgtctctgg
     4381 gatcagcaag acggtgaagc cgtcgtcgtc gactgcggcg atcgtgagcg acacgccgtc
     4441 gaccgccacc gagcccttct cgaccatgta acgcagcagg gccggttcgc aggcgatacg
     4501 cacgagcttc gctatcccct cgtcggtcac cgattcgacc gttccggtcc cgtccacgtg
     4561 gccctgcacc atgtggccgc cgaggcggtc gccggcgcgg agcgcgagct cgaggttcac
     4621 gcggtcgccc tcgccgagcg ggccgagcga cgagcgccgc agcgtctcgg ccatcacgtc
     4681 ggcgcggaag ccgccgtcag ccacgtcgac cgccgtcagg catacgccgt tcacggagat
     4741 cgagtcgccg cgcccgagct cggaggcgag ctcggtggcg acctcgaggc gcacgccgtc
     4801 ttcggagcgc tcgatcgcgc gcacgctgcc ggttccctgc acgaggccgg tgaacatcac
     4861 cactccctca tgcgggccgt gatgagcagg tcgtccccga tgcgctcgga gtcgatcgcg
     4921 agcgcgcgat ccgcggcctc gatcgtctcc acgccctggc cctcgagcgg cactcgggcg
     4981 cggcgcccgc cgagcagcat cggcgcgatg aagatgcgcg cctcgtcgat ctcgccggca
     5041 tccatgaacg cgcccgccag gtgcgggcct ccctcgagca gcaccgactg cacgccgagc
     5101 tcgccgagct ggctcagcgc cgactgcacg cgcgcggcct cgttgccacc ggtcgcgacg
     5161 atcacctcgg cgcccgccgc ctcgagcgcg gccacggcgc gccgcgaggc ggcgcgtgag
     5221 gtgaccaccg tgagcggcac ctcgttcgcg ctcgccgcga gctgcccctc cgcgggcagg
     5281 cgcgcttcgg agtcgaagac cacacgacgc ggctggcgcc tgacgccctc tacgcgcgcg
     5341 gtcaacagcg gattgtcggc gagcgcggtt ccgatgccca ctgcgaccgc gtccatctcg
     5401 gctcgccagc ggtgcgcgcg ggcgcggctg gcgtcgctcg agatccactt cgagtcgccc
     5461 gtgtgggtgg cgaccttgcc gtcgagcgtc atcgcggact tgaacaccac gagggggtgg
     5521 cccgtcttcg cgtgcttgcg gaagggctgg ttgagcagcc gggcggcttg ggctgctccg
     5581 ccggcggcca cgtcgacggt gatcccctcg tcgcgcagga tgccgaggcc gcgtccggag
     5641 gccttctccg tggggtcgtc gctcgccacc acgacccgcc cgacgcgcgc gtcgatcagc
     5701 gcgtccgtgc acgggggcgt gcgcccgtga tggcagcacg gctcgagcga cacgtacatc
     5761 gtcgcgccgg ccggatcctc gctcgcgctc gcgagcgcca tgcgctcggc gtgcggctca
     5821 ccggcggcgg cgtggaaacc ctcgccgatc acgcggccgt ccttgacgag cacggcgccc
     5881 acgagcggat tcgggctcgt catcccgcgc gcccggtccg cgagctcaat ggctctcaac
     5941 aggtggcgct cgtcgcgctg cgtcgcggtc agctgggtca ttcccgggaa aattgactcg
     6001 cgagtcaact ttaccaatgc catgtagggt ccccgcccat gcgacgcaca cgcgccgccg
     6061 cgtggcccgc gtatgcgatc ctcgctctga tcctcgtcgc gggcctgtat ttgcggctcc
     6121 ggcacaacgg ctatggcctc ccttacgtct acaaccagga cgaggggcgc cacttcacca
     6181 accgcgccgt ggcgatgttc ggcggcggca gttgggaccc cggctactac gagaacccgt
     6241 cgggtttcac ctacctgctg aacatcgcga tgcgcttccg ctacggccac ggctggccgt
     6301 tcggaggctt cgacaacgtc gtgcgcatgt tcgcgagccc cgacccgagc ggcgtgtacg
     6361 agctcacgcg cggagtggcc gcgctgctgt gctgcgcagg cgcggccggc gtgttcttcg
     6421 tcgggcgccg cctctggggc ctgcgcgagg ggctcgttgc tgcggccgtg ctcgcgttcg
     6481 cgttcctgcc tgtcgcctat tcgcgcatcg cggtgaccga cgtcggcacg ctgcttcccg
     6541 tggcgatcgc gctcttcgcc gcggtgcgga tctacgagcg cggtgacctg cgctggtacc
     6601 tcgtgggtgg cgccgccgtc gggttcgcga tcggcttcaa gtacacggcc gggctcgtcc
     6661 tgctgccgct gctgatcgcg acggcgtttc gcatgcgcac cgacgggctc gctccgctga
     6721 agggactcgg gatcgcgctc gtggtcgcga cggcggcctt cttcgtcacg acgccgtact
     6781 tcttcatcaa cttcagccac gccaggcacc agctcacgtc gcagtcgcag acggtgggcg
     6841 acttcggcaa ggtcggccaa gcgcaggaca acggcttcgc ctactacctc tggagcctca
     6901 cctgggggct cggctgggtg ccgctcgtag cggcggcggc gggatgcttc ctcgtggcga
     6961 ggcgcgatcc cgtgcgcgcg ctcctgctcg cggccttccc gctcgcactg ttcatctacc
     7021 tcagcgtgca gtcgcgcttc ttcgggcgct ggctgctgcc ggtctacccc gctctggcgc
     7081 tcttcgcggg ctacgcgatc gtgcaggcgg tgggtgcggt cgccgcgctg cgcgggcggc
     7141 ggctcgcggc ggcgggcgcg ctggcggcgg tggtgctcgc ggcgctggcc cagccgctcg
     7201 ccgccgacgt gcgcacgatg cggctgctcg ggcgcaccga cacccgccag ctcgcgcgcg
     7261 actacctcgt gaagacctac ccgccgaggc tgcggatcgt gatcgagcct gccgtcccgg
     7321 ggcgctacta ccggctcgcg cgcccaggtc ggaaggcgcc gccggagcgc aagcagttcg
     7381 tgcgcgggtt catcagggac atcggcgaga cgcgcatcga ctacgtgacg ttgctgcgtc
     7441 ccgaagtcct cgaccattac cgtcgtagcg gctattgcct cgtgatgacg atgagcgtca
     7501 ttcgcggcag ggccgaaaat gaccgcctgg cgcccgccct cgcgtactac gcacggctcg
     7561 aacgcgagtc gaagctggtc ttcgaggcca gcccctacga caagggcgtc aagcacctca
     7621 agttcaactt cgaccactcg tacgactact acccgagcaa gttcgcgcgc cccggccccg
     7681 acgtgaagat ctaccgcctc aacaactgca agcagggcta cgggccgctg ccgaagggcg
     7741 cgggcgtgcc gcagggggtg agcgagtgac ggccgaggcg aagccgcgca caaccgcccg
     7801 cggggcgacg ctgctcgagc gcgtccgccg cccgaccgca gcaggctgga ccctcgctgg
     7861 gatcctcgtg ggcgcgctcg tcctgcgact gtggtcgatc cggcacggta tgccctgggc
     7921 gttcaactac gacgaggagc accacttcgt gccgccggcc gtgtcgatgc tgggcggctc
     7981 gctgaacccc ggctacttcg agaacccgcc cgcgctcacg tacctgctct tcggtgtctt
     8041 caagctgcgc ttccacgccg gcttcccgtt cggggcgagc ggcttcgtgc ggcatttcaa
     8101 gaacgacccg gagtcggcgt tcgtcaccgc gcgcatcgtg gtcgcgctgc tcggcacgct
     8161 cgcggtgtgg ctcgtgcact gggcgggttc gcgctactac ggccggcgtg tgggactgct
     8221 cgccgcggcg ctcgtcgcct gcgcgttcct gcccgtcttc tactccaagc aggcgctgaa
     8281 cgacgtcgtg acgctcgtac cgctcaccgt ggcgctcggg gcgtgcctgc tcgtgtacga
     8341 gcgcggccgg gcgctcgact gggcgctcgc cggcggcgcg atcggcgtgg cgaccgccac
     8401 gaagtacacg gcgggagcga tggtcgccgt gctcgcgatc gcggcgctgc tgcggctgcg
     8461 cgaccggcgc gacgacctcg gggcgctcgt tcgcggcgct gtgatcgcgg gcgtgctgtt
     8521 cgtcctgctc ttcctgctgc tcaaccccta ctcgctgatc gacttctcga agttcaagtc
     8581 gcaggtgggt ggccagtcgg cgacggccgg agggctcgcg aagctcgggc aggacgacgt
     8641 gcctggctgg ctctactact tgtggacgct cacgtggggg ttcgggtgga tcccgctcgc
     8701 ggccgcggtc gggggcgccg tcgttgccct gcgctcggac tggcgccggg gcgcgctgct
     8761 catcgtcttc ccgatcgtgc tgtggctgtt cctgggcggg caggcgcgct atttcggacg
     8821 ctggttcctc cccgcgtacc cggccctggc gatcctcgcc gcctacggga tggtgcgcct
     8881 cgtggattgg ttcgtcgccc gtcgcggcgc cgtccaggcc gcgccggcgc tgctcgtggc
     8941 cgcgggcttc cttctcgtcg tccagggcct ggccgcaagc gtgcgcgtgg acagcgtgct
     9001 cgcgcacacc gatacgcgca cgctcacgcg cgagtggctc gcgcgcaacg tgccgcaggg
     9061 ctcggggctg gtggccgagc cgttcttccc gagcggcttc ttcaccaccg gcggacggcg
     9121 cgcgcccgac cgctaccggc tctacccgat caagccgccg ttccaggggt acgagaagaa
     9181 gctgacgccc gggctgatcg acacctaccg ctcgcagcgc tactgctggg tcgtgaccgg
     9241 tacctatcag gagggccgcg gcctcaaggc cgggctcgcg aacgcgaagg cctactactc
     9301 gcggctcgac gccgagtcgg agacggtcgc tgtcttctca ccctggtacg ccggcgaggg
     9361 gcccgtcgag ttcaacttcg accactcctt cgactactac ccgcgcgcct acaggcgtcc
     9421 ggggccgctg atcgaggtac accacctgaa cggatgccgc tagaccgccg caacatcgcg
     9481 aagcgagtgc tgctcgcgcc ccacttcgtg cgccgggcgg cggcgagcca agcggcggcg
     9541 cgcgaggagg gatggctcct gcgccagcca aaggacgtcc gcgcgagcta cgtgcgcgag
     9601 gttctcgaca aaggagatgg cccgcgcctc gccgagatct ggatgctgcg ccagcggcga
     9661 gaggtgcgcg agagctacat ccgcgaggtg ctgggggcgg actaggcgcc cagcaccagc
     9721 tctgccattg gcctgatcag cgcggggtcg gcgccgatca gcagcgtcgt caccatcgag
     9781 tcccgccact tctctatgtc gtggcggatc ttgtccgggg acccgatcag cgccaggcgc
     9841 tcgatgagct cggtcgggac tgccgcggcc gcctcttcct tcttgccgtc gagatagagc
     9901 tcctggatct cgtcgacctc ggcgtcgtag cccatacggc gcgcgacgtc ggcgtggaag
     9961 ttcgctttct tcgcgcccat cccgccgaag tagagcgcgt agagagggcg catccggtcg
    10021 gcggcgcgct cgacgtcgtc atcgatgacg acgttcacag aggccgcgat ctcgaagtcg
    10081 tcaaacgagc gccgcgcacc ctcgcgggcg aagccctcgg cgagcgcgtc gcggtagaag
    10141 cgctcgtggt cgggggagaa gaacagcgcg agccagccgt cgcagagctc ggccgctagc
    10201 gccacgttct tcgggccctc ggcgccgagg aagatcggga tgtcctcgcg gagcgggtgg
    10261 atcgacgact tgagcggctt cgcgaggccg gtgccgcctt cgtacggcag ccggtagtgc
    10321 tggccctcaa aagtgacggg gccgttgcgg gcgaggatct ggcggatgat cgagatgtac
    10381 tcgcgggtcc gggcgagcgg ctttgcgaat gcctggccgt accagccctc taccacctgc
    10441 gggcccgaca ctcccagccc gaggatgaag cggccgcctg agaggtggtc catcgtcatg
    10501 gcagccatgg cggttgccgc aggcgtccga gcgctcatct gcacgatcgc cgtcccgagc
    10561 cgaatgcgct cggtctgtgc tccccaccag gcgagcggag tgagcacgtc cgagccgtac
    10621 gcctcggccg cccatagcga gtcgtagccg agccgctcgg cctccttcac ggtctcggcc
    10681 gcgttcggcg gcggcccgcc ggcccagtag cccaggttga ggccaagctt gagctcgcgc
    10741 gccattcgat cattcgggcg tcttcacgcc ctcgcgcagc atctcgacgg ctttcttgac
    10801 gcgccgctca cgagtgtcgt cccgcttcgc ttctgcgatc cagcgcgcgt actccttgcg
    10861 gtgcgtgaag gcgagctggt cgaatgcttg gcgcgcgtcg tcggcccgtg cgagcgcgtt
    10921 ggcaagcgcg tccggcacct cgacctcccg cggctgggcg tcgagcgcga gcaccacttc
    10981 cacgtcgtcg cccagctcga tgccggccgc ctcacgtatc tccctgttga ggccgagcac
    11041 cgcgcggccg ccgtagacag cgatccggcc gcggaactcg gtgccgttca gagtgccccg
    11101 taccggcgcc cgcgcttgcc cgaacttctg cttcggatcg aacggcacct cgagccagcg
    11161 gccaccgccg ccaccgtccg cttccgtgag ctttgcgctg aagcgctcgg gcacgtgcgg
    11221 cagcctaacc ggcgcacggc gcccggaccg gcaccttttc gttttcagag ttgcggagcg
    11281 caacgatgca aagaaggtgt cggtcgggcc ccgggcgcct caggcgcctg gacagttggc
    11341 gtagacgcgc cacatggagt tgcccgcgac gaacttgaac gccgagagcg ggtagttcga
    11401 ccaccacttc cgcgccgact tctggttcac gccggtgacc gagcgcgccg cggcaggctc
    11461 gaagttgaag ctcgagccga ggaagagaag gccgtgcgtg ggcggaccgg tctggttgat
    11521 cgaggcctgg aggtcgtgct tgccgagtcc ggtctcccac tccaccacgg gtagggccga
    11581 gtgcgtgggg aacgtgatcg ggcggcactt cgccatcagc gcggtgacgc tcggatcctg
    11641 gaggatcgcc ttcagcccct ggtgctgctt cttcacgaac agcgtctgcc cgttgagctt
    11701 gcggaagtcg ttgatccagg caggcgtgcg ccacatcacc agcagcagcg acagcgccgc
    11761 gacgccgatc gcgactcggc gcgcgcgccg gtcggtgatc accgcccagc cggcgagcgc
    11821 cacacccacg ccgaggttga acagcagcga cggaatcacg aggtagcgag ggatcaccga
    11881 cagcccggcg atcgcgatca tcaggaagac gccgatgccc acggccatca gcgccagcgg
    11941 aaggtaggcg cggcggcgca gcatccagag cgcgagcagc agtccgaggc cgccggccgc
    12001 cacgttcacg atcttctcgt tcgcgcccac gtagtccggg atcagcctca ccgcctcggg
    12061 cacgctgcga tcgcggccga actggccggc gacgtcgcga gtggaggtga gcgaatggag
    12121 cggatggccg gtgacgatcc agtccgaggc gatccacaac agcggcgcgg ccgccacgag
    12181 cagcgcgtag cggatgaact gctcgcgact gatcgccggc cagagccaca gcaggtagac
    12241 gcccgacagc acccaggcct ccgggcggag cagtccggcc aagccaagca gcaccagcac
    12301 cggcagcccg cggcgcggcg atttgagctc ccacaacgcc gccccgaaca cgagcaacag
    12361 gaacggcacg tcgaccatcc cgcgcagcgc gtagaagatc aggtcggcgc gcgtgcagac
    12421 caccgccacg ccgaccagcg cgatcaacgt gccgagcagg tgctgtgcga agcggaaaag
    12481 caccacgagg aggccgatga agctcgcgat cgtgatgagc acgagcagcc ggtccgccgc
    12541 cccgccgaag aacgacagcc ccgccccgag cagctcccag agcggatgag gtgtgggcgt
    12601 gcggaagacg tcgtagtcgg gcaggtgccc gtgcgacagc gcgcggcccc agacgagcga
    12661 gtagtagctg tcgtagttgg ggtaggtcgg ccggaagagg aagatcccga gcgagaccgc
    12721 ggagacgaag gcgagcaacc gcgcgccgtt gcggcgcgtc gccagcgcct cagcgcgggt
    12781 ggctaggcgc gactcgcctg cgagcccggc cgcgggcggc acggtggctg gctggggcgc
    12841 gccgcgggtg atcagtttgc tcacgaacac agcggcttca cgggagccgg cggcgagcag
    12901 gtgacggggg gcagcgcgcc gctcggtgag agtcggcaga ataccagcgg tacccgccgt
    12961 taacggcggg attctccgtg ccttctggcg gcgcggagtc agcgccggaa gccgccttct
    13021 cgatccgtgc tccggcctcc gatccctgcg ctagcctgaa cccgccccga tgaagctgat
    13081 catccagatc ccgagctgga acgaggagga gacgctcccc ggcacgcttg ccgacctccc
    13141 gcgccagatg cccggcgtcg acgtggtcga gtggctcgtg atcgacgacg gctccacgga
    13201 ccggaccgtc gaggtcgcgc gcgagcacgg cgtcgaccac atcgtgcgcc tcacgaacaa
    13261 caagggcctg gcggccgcct ggcaggcggg cctcgacgcc gccctgaagc tcggcgcaga
    13321 tgtgatcgtc aacacggacg ccgacaacca gtacaagggg tcggaaatcc cgaaactcgt
    13381 cgcgccgatc cttgccggcg acgcggacat ggtgatcggc gaccgccagg tcgacacgat
    13441 cgagcacttc tcgaacctca aggtgaagct gcagcaactg ggtagcgccg tggtgcgccg
    13501 cgcctcgggc acgcacatcg aggacaccac ctccggcttc cgcgcctaca accgcgaggc
    13561 ggcgctcgcg atgcaggtgg tctcgaagtt cacctacacg ctcgagacga tcatccaggc
    13621 cgggaagatg accgtcgcgg tcgaccacgt gccgatcggg accaacccga agacgcgcga
    13681 gtcccgcctc ttcccctcca tgtgggcgta cgtgcgccgc aacgccgtct cgatcttccg
    13741 catctacgcc ctctacgagc cgctgcgtgt gttcatgacc gcggcgatca tcgtcggcat
    13801 cgccgccgcg gcgatctgga tccgtttcct cgtcgcctac atcggcggct cgggcgcggg
    13861 tcatgtccag tcgctgatcc tcggtgcggt gctgttcatc gcggccgtgc agctcgcggc
    13921 tctcggcgtc atcggcgacg tgctcgccgg cagccgcgtg ctgtcccagc gcatcctcga
    13981 gcgcgtgcgg cgcgtcgagt tgcagctcgg catcgagccc tcgcactatg agccgggcaa
    14041 ggcgagcacc ggccaggagg ccaccaccgg cgccgacgcc ggccccgcca ccggcaagcc
    14101 cgaggaggaa tcgaccgagg agcgcgaggc ggtgaagctg tgacccaggc ccccatcgag
    14161 gccgtcccca ccggcaacac cttcgacaag tacgggtcca cgaacccggt cgtgcgcagg
    14221 ctcatggagg gcttccagtc gacgctcgac gagctgtggg cgaaggccgc gccgaactcg
    14281 atcctcgacg tgggctgcgg cgagggcgtg ctcacctacg agtgggcaga gcgcctcggc
    14341 gatcgccgca tcgtgggcat cgacctcgac gacgacaagc tcaaggccga gtgggacacg
    14401 cgccagcgcg cgaatctcga atacgtgccc gccgacgcca cctcgctcga gttcgtcgat
    14461 gacgagttcg acatggcgag cgcgatcgag gtgctcgagc acgttcccga gcctgagcgc
    14521 acgctcgccg agatggcgcg cgtcgcacgt cgccacctgc tcgtgtcggt gccgcgggag
    14581 ccgctctggc gagggctcaa catggcgcgc ggcgcctacc tgaaggactt cggcaacacg
    14641 cccggccaca tcaaccactg gtccaagcgg tcgttcgtgt cgctcctctc ccgctacggc
    14701 acggtcgagg aggcccgctc gccgttcccg tggaccatgc tgcttgtccg cctcagctag
    14761 ctcgccctcc gcgacccgct cgtacgggcg gggcgcatcg atcctcgcgg tcggcatcgg
    14821 ggtcaccggc ctcgtcacct actcgtactt cgcgctagcg agccacacgc tcgacgcgac
    14881 cgactacggc cagatcagcc tgctgtggtc ggcgatcttc atcgtcgtct cggtgctcta
    14941 ccggccggtg gagcagctgc tctcccgcac gatctccgag cgcgacgcgc gcggtcagca
    15001 cggcaacgag cacctgcggg ttgccgccac gatccagctt gcgctcggcc tggtgttcgc
    15061 cgtcgcggcg ctcgccttca gggacagact gcagaaccac ctcttcggcg gctccgccac
    15121 cctctactgg atcctgatca tcgccgtgct cgcgtacgcg gcgagctact tcgcgcgtgg
    15181 cttcctcgcg gggcaccggc gcttcggcct gtacggcggc ctcgtgctga tggaggcgac
    15241 ctctcgcttc ctgttcgcac tcgcggtcac ggtcggcatc gctcacggcc agg
//
LOCUS       DASVXS010000055         8709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_438334, whole genome shotgun sequence.
ACCESSION   DASVXS010000055 DASVXS010000000
VERSION     DASVXS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438334"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VF752_03005"
     CDS             <1..745
                     /locus_tag="VF752_03005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8854542.1"
                     /translation="LSGGRMVLGLGVSHRVTVENWYGTTIDRPVAELREYVESLRAIF
                     RDEDPPEGSRFPTRFHFMGYPVRADLPIYVAALSPTMLRLAGRVADGVVLWLCNPEYI
                     ERVVVPEVKIGREKAGKTLEGFDIVAAVPAAVTDDRAATEALRNELIPYFSLPFYRAA
                     IERSGFEDEIAAYDEAMAAGDPQAAGAEISDRFIASLAAIGSADEAAATVRRYLDAGA
                     TSPCIGPVPRTDPELTLQALSGLAPSPQA"
     gene            781..1968
                     /locus_tag="VF752_03010"
     CDS             781..1968
                     /locus_tag="VF752_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854543.1"
                     /translation="MAEAPRATEAVTREIPSDTLATLAHEIPTSLLPALVTGILAASP
                     AALAAIEGIANAMGIGARLAGDLRSRDPDKRRRLRLGGYTGTAALSSLTGVSGTALVA
                     GLLRTGAWAARGVASPERHVQVAQRTQRSSLGQAYARGRLLEHAAAVAGPIIACLLLL
                     GVGIRTAIVLSVVPGVLALAFALRGWRRVPSEVPPPATPITVSRLRDLVSGKLGWSFL
                     GIAAFEFGNITVVLLILRATALFRPDHGLVGAARMAALGYALYRLAASAASGAAGRLV
                     DRIGAAPPLAAGSAILLASYTGFAFVPDNIPGLAACFATAGVAVGLVEPSEQTLIALH
                     APPDERRSLFTLLAVVDGVGVVAASLIAGVLWTVFSPAAGLLWSAPALLLCVLALGRA
                     HTR"
     gene            complement(1965..2399)
                     /locus_tag="VF752_03015"
     CDS             complement(1965..2399)
                     /locus_tag="VF752_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854544.1"
                     /translation="MGAPRRGVSHAASAAVAVAALGAFVLAGCGGKDQGPNARKPAQE
                     AGAVVNTFERDLLAGDHQHICNEIFSSQVREQAGGRGCAGFLRRTAGPVKRSRIEVKA
                     VALMGDTAKVRVETSASGQARVAETIELVRQGGRFRIASLSR"
     gene            2421..3032
                     /locus_tag="VF752_03020"
     CDS             2421..3032
                     /locus_tag="VF752_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854545.1"
                     /translation="MEGSAPESLGTSSSWLRCYRCWSRNLEVQVHYEGVLKVDPETGE
                     AVDPVEEVQEAVVQCLDCLHDQPHLSLAFYEEDGRRISRIEAVEDRWERMVAGTPWVA
                     SCTVTVDGDQVEACSGPEAADALAYGAFGDHGTREFFTHVRFHKHEADQIVIHMLVEL
                     YARSAEEAADVLEQASRGELAITSLAEESRPPAASASGGDDPH"
     gene            complement(3029..3634)
                     /locus_tag="VF752_03025"
     CDS             complement(3029..3634)
                     /locus_tag="VF752_03025"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8854546.1"
                     /translation="MSELEKALRRDAVVADLDALTHRFAERAAGEGLLDIAYAELDSP
                     LGRLVAASTDRGLVTLSYADSGVDPVLERLAAMISPRVLEAPARLDDVQREVDEYFEG
                     RRHDFDLPIDWALVHGFTARILQATARIPFGEVSSYRGVAREAGNERAVRAAGNALGA
                     NPIPIVVPCHRVLRTGGALGGYTGGVERKKHLLALEGVLPR"
     gene            complement(3631..4092)
                     /locus_tag="VF752_03030"
     CDS             complement(3631..4092)
                     /locus_tag="VF752_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8854547.1"
                     /translation="MPPFQVFLEEHRDRVFRFLVASVGPHDAEDCFQDTFISALRAYP
                     RLRPDSKLSAWVLTIAHRKALDVHRARARHAIPVDELPERGVEDQPRDGEHGLWTSAR
                     DLPAKQREALLYRFAGDLPYADVAAAMGVSEDAARQSVSQGLKKLRKELVG"
     gene            complement(4447..5991)
                     /locus_tag="VF752_03035"
     CDS             complement(4447..5991)
                     /locus_tag="VF752_03035"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX8854548.1"
                     /translation="MAKQLSPLPDAKSVRVNPSAAELKELAAKMPNARRTKYDNLNVQ
                     TEVLARSKASTFLVLDDPDGSSQQAISREEGERIARLQDEYIAGRDMVIVDGYIGNDP
                     EFRTPARLYIEESNANIAGMQKQLYFDVDDGDGFQPELTVIYTPHLAVEGYPNDRVIA
                     VDLERGVTRVCNSDYFGESKKGGLRMWNKLVFDRGGLPLHAGCKVIPTDDGDKVGLIV
                     GLSGTGKTTTTFTRQNGSLPVQDDFVAMMPGGKVYATEAGCFAKTFALSQEDEPTIYG
                     AVTTPEAYLENVSQDDNGDLDFFDTSYTQNGRATFSFSSIEAADARDLQSADFLLILN
                     RNENIIPAVAKLDAEQAAAYFMLGETQGTSAGGADEAGKFLRVPGTNPFFPDDDDHQG
                     NRFLELMAEHQLDVYLMNTGRVGGAEADERSVKVKIPHSSAIVKGIAEGTIEWEEDPD
                     FGYEVAKSVPGIDPSDPVLRPRELYAEQERIEEYDRAVEHLKRERVAFLEKFPALTQE
                     IVDAVR"
     gene            complement(6119..7804)
                     /locus_tag="VF752_03040"
     CDS             complement(6119..7804)
                     /locus_tag="VF752_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8854549.1"
                     /translation="MTALDAQAGAPSLGEEARAAAADRSVMARTLAYVFAVGSTLAVV
                     SLALPSSVDVPNVGVAAPLGALAAVATAALLAVLVLGAKRLRAWAFHLVLACGTLLVS
                     AAIYWSNNATSTYPMLYVPIALYAAYFFTPREAAVQVGVAAASYAVVLLASDADHGSV
                     AAWLLTVGTLATTAALVRTMRDRLERLIGRLSDAARTDVLTGLLNRRGFQELFGNELE
                     RATRSNRPLSLIVGDIDHFKDLNDRFGHRAGDDALERLASVLRAAKRRIDTAARVGGE
                     EFALIVPETEEHDAYVLAERLRRAVREQFEDSDRRVTISFGVASFPKHGRTAEALFHA
                     GDLALYAAKELGRDRTVIHNPELAGAIAGTRRRFERDGYLATVLALAEALEARESGSA
                     THSRRVARYAELIARELGLGEEVVDRVRIGGVLHDVGKIGVPESILSKNGPLSEEEWE
                     ELKRHPLIAARILDSGSIEDIRAWVLAHHERFDGTGYPHGLAGEEIPMEARILSVADA
                     WEAMTSDRPYRRALTTDEARAELLACSGSQFDSEVVRAFLRVLDGSDVESLVP"
     gene            7904..8584
                     /locus_tag="VF752_03045"
     CDS             7904..8584
                     /locus_tag="VF752_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX8854550.1"
                     /translation="MRQRSTHVRAVVRFLAAVVAVAGALLIADAVITVAWQEPISAMI
                     ASREQDRLEHQLGALETQSAADARSVNVRGLRHRVAVLAARARARAKTGHAIGTIRLP
                     TLHRHYVMVQGTDTGSLRRGPGHYPATPFPGQRGTTAVAGHRTTYLAPFRTIDQLKKG
                     NRIVADMPYGQITYEVESTRIVKPTALWVTRKVDHDRLVLSACHPLYSASHRIVVFAR
                     LVSVAPPA"
BASE COUNT         1282 a         3097 c         2993 g         1337 t
ORIGIN      
        1 gctgtctggc ggccggatgg tgctcggtct cggcgtctcg caccgcgtga ccgtggagaa
       61 ctggtacggc acgacgatcg accggccggt cgccgagttg cgcgagtacg tggagtcgct
      121 gagggcgatc ttccgcgatg aggatccgcc cgagggctcg cgcttcccca cccgcttcca
      181 cttcatgggc tatccggtgc gagcggacct gccgatctac gtcgcggctc tctcacccac
      241 gatgctgcgg ctcgccgggc gcgtcgccga cggcgtcgtg ctctggctgt gcaaccccga
      301 gtacatcgag cgcgtcgtgg tgcccgaggt caagatcggg cgcgagaagg ccggcaagac
      361 gctcgaaggc ttcgacatcg tcgcggccgt tccggcggcg gtcaccgacg accgcgcggc
      421 caccgaggcg ctgcgcaacg aactgatccc ttacttctcg ctcccgttct accgggcagc
      481 gatcgagcgc tcgggcttcg aggacgagat cgcggcatac gacgaggcga tggcggctgg
      541 tgacccgcaa gcggcgggcg ccgagatctc tgaccgcttc atcgcctcgc tggcggcgat
      601 cggcagcgcc gacgaggcgg ccgcgacggt ccggcgctac ctcgacgcgg gcgcgacgtc
      661 gccctgcatc ggtccggtgc cgcgcacgga ccccgagctc acgctgcagg cgctctccgg
      721 gctcgcgccc agcccgcagg cctaacgccc cgcgctgcgc ggggcgtagc cttggctcga
      781 gtggctgaag caccgcgcgc caccgaggcg gtcacgcgcg agatcccatc cgacacactc
      841 gcgacgctcg cccacgagat cccgacgtcg ctgttgcccg cgctcgtgac cggcatcctc
      901 gccgcgtcgc ctgccgcgct ggcggcgatc gagggaatcg cgaacgcgat gggcatcggc
      961 gcgcgcctcg ccggcgacct ccgctcccgc gatccggaca agcgccgacg actgaggctc
     1021 ggcggataca ccggcaccgc cgcgctgtcc agcctcactg gcgtgtcggg caccgcgctg
     1081 gtcgcagggc ttctccggac gggggcatgg gcggcgcgag gcgtcgcaag ccccgagcgt
     1141 catgtgcagg tcgcccagcg gacgcaacgc tcgtcgctcg ggcaagcgta cgcccgcggc
     1201 cggttgctgg aacacgccgc ggcagtcgcg ggacccatca tcgcctgcct gctgctgctc
     1261 ggcgtgggaa tcaggaccgc gatcgtgctg tcggtggtgc cgggagtgct agcgctcgcg
     1321 ttcgcgctgc gggggtggcg gcgcgtgccg tccgaggtgc cgccgcccgc gacgccgatc
     1381 accgtctcgc gcctgcgcga tctcgtctcc ggcaagctcg gatggagctt tctcgggatc
     1441 gcggcgttcg agttcggcaa catcaccgtg gttctcctga tcctgagggc cacagcgctg
     1501 tttcgccctg atcacggtct cgtcggagcg gcgaggatgg ctgcgctcgg gtatgcgctg
     1561 taccgactgg ccgcctccgc ggcgagcggt gccgccggtc gcctggtgga ccggatagga
     1621 gcagcgccgc cactcgctgc gggctcggcc atcctcctcg cctcctacac cgggttcgcc
     1681 ttcgtccccg acaacattcc agggcttgca gcgtgcttcg cgactgccgg cgtggccgtc
     1741 gggctcgtcg agccgtcgga gcaaacgctg atcgcgctgc acgcgccgcc ggacgagcgc
     1801 cgatcgctgt tcacgcttct cgcggtagtg gacggtgtgg gcgtcgtcgc ggcgagtctc
     1861 atagccgggg tgctgtggac ggtcttctcg cccgccgcgg gccttctgtg gtcggccccc
     1921 gccctgctct tgtgcgtgct ggcgctgggc cgggcgcaca ctcgctagcg agagagcgag
     1981 gcgatccgaa agcggccgcc ctggcgcacg agctcgatcg tctcggccac gcgagcttgc
     2041 ccgctggcgc tcgtctcgac ccgcaccttc gcggtgtcgc ccatgagcgc gacggccttc
     2101 acctcgatac gagaccgctt cacgggcccg gccgtacggc gcagaaagcc cgcgcagccg
     2161 cgcccgcccg cctgctcgcg gacctgcgag ctgaagatct cgttgcagat gtgctggtgg
     2221 tcgccggcca gcaggtcgcg ctcgaaggtg ttcaccaccg cacccgcttc ctgagcgggt
     2281 ttgcgcgcat tcgggccctg gtccttgccc ccgcacccgg ccagcacgaa cgcccccagc
     2341 gccgccaccg caaccgccgc cgaggccgca tgtgagacac ccctccttgg cgctcccata
     2401 tgcgctgtca gaatagaggc atggaagggt ctgcgcctga gtccctgggg accagctcga
     2461 gctggctccg ctgctaccgc tgctggtcac ggaacctcga agtgcaggtg cactacgagg
     2521 gcgtcctgaa agtcgatccg gagacgggtg aggcggtcga tcccgtcgag gaggtgcagg
     2581 aggcggtggt ccagtgcctg gactgcctgc acgatcagcc gcacctgagc ctcgccttct
     2641 acgaggagga cggccggcgc atcagccgca tcgaggcggt cgaggaccgc tgggagcgga
     2701 tggtggcggg gacgccctgg gtggcgtcgt gcacagtgac ggtcgacggc gaccaggtcg
     2761 aagcctgctc gggtccggag gccgctgacg cgctggccta cggggcgttc ggcgaccacg
     2821 gcacgcgcga gttcttcacg cacgtgcgct tccacaagca cgaggccgac cagatcgtga
     2881 tccacatgct cgtcgagctc tacgcgcgct ccgccgagga ggccgcggac gtgctcgagc
     2941 aggcctcgcg cggcgaactg gcgatcacgt cgctcgccga ggagtcgcgc ccgcccgccg
     3001 cctccgccag cggcggcgac gacccgcact agcgcggaag cacaccctcg agcgcgagca
     3061 ggtgcttctt gcgctcgacg ccgcccgtgt agccgccgag tgcgcccccg gtgcggagca
     3121 cgcggtggca cggcacgacg atcgggatcg ggttcgcgcc caacgcgttg cccgccgccc
     3181 gtaccgcgcg ctcattgccc gcctcgcgcg ccacgccacg gtagctcgac acctcgccga
     3241 acgggatgcg ggccgtggcc tggaggatgc gcgcggtgaa cccgtggacg agcgcccagt
     3301 cgatcgggag gtcgaagtcg tggcgccggc cctcgaagta ctcgtccacc tcgcgctgca
     3361 cgtcgtccag ccgggccgga gcctcgagca cgcgcgggga gatcatcgcg gcgagccgct
     3421 cgagcaccgg atcgacgccc gagtcggcgt agctcagcgt gacaagcccg cggtcggtgc
     3481 tcgcggccac cagccgcccg agcggcgagt cgagctcggc gtacgcgata tcgagcaggc
     3541 cctcgccggc cgctcgctcc gcgaaccggt gcgtgagcgc gtcgagatcc gcgaccacgg
     3601 cgtcccgcct caatgccttc tccagctcgc tcatcccacc agctccttcc tgagcttctt
     3661 cagcccctgg ctcacgctct ggcgtgccgc gtcctccgac acgcccatcg ccgccgccac
     3721 gtcggcgtag ggcaggtcgc ccgcgaagcg gtacaagagc gcctcgcgct gcttcgcggg
     3781 taggtcacgc gcgctcgtcc agagtccatg ctcgccgtct cttggctgat cctccacccc
     3841 ccgctcgggc agctcgtcga cggggatcgc gtggcgcgcg cgagcgcggt gcacgtcgag
     3901 cgccttgcgg tgggcgatcg tgagcaccca ggcgctcagt ttcgagtccg ggcggaggcg
     3961 cgggtatgcg cgcagagctg agatgaacgt gtcctggaag cagtcttctg cgtcgtgggg
     4021 cccgacgctc gcgacgagaa accggaacac acgatcgcgg tgctcttcga ggaagacctg
     4081 gaacgggggc aatggcactc gtgtattgaa acgcccccag ttctcgaaac gtgagaaacg
     4141 gcgggcagtc aaggaggcgg ggcatgaatt tctgcaggcg accggctcct cggcgaaacg
     4201 cgcctgcagt tattcacgcc atacatgaat ttctgcaggc gacggagacg acgcgcccac
     4261 tcgcctgcag ttattcatga cccggcccgc ggagagcgcg gcgctgtggg tcacccccac
     4321 cccaccctca ctgctcttcc gaagaacagc gggcagccgc ggacgagcgg acggcgccag
     4381 cccaaaacgc ggagagcccc cgcttgtgcg agggccctcc gcaccttccg gctgtcccag
     4441 attcgatcag cgaacggcgt ccacgatctc ctgagtgagc gccgggaact tctccaggaa
     4501 ggcgacgcgc tcgcgcttga ggtgctccac cgcgcggtcg tactcctcga tgcgttcctg
     4561 ctccgcgtag agctcgcgcg ggcgcagtac cggatcggag gggtcgatgc ccggcaccga
     4621 cttcgcgacc tcatagccga agtcagggtc ctcctcccac tcgatcgtgc cctcggcgat
     4681 gcccttcacg atcgcggagc tgtgcgggat cttcaccttc accgatcgct cgtcggcctc
     4741 ggcgccgccc acgcggccgg tgttcatgag gtagacatcg agctggtgct cagccatgag
     4801 ctccaggaag cggttgccct gatggtcgtc atcgtccggg aagaacggat tcgtgcccgg
     4861 gacgcgaagg aacttgcccg cctcatcggc accacccgcg ctcgtgccct gcgtctcacc
     4921 gagcatgaag tacgcagccg cctgctccgc atccagcttc gcgaccgccg ggatgatgtt
     4981 ctcgttgcgg ttgaggatca gcagaaagtc cgccgactgg aggtcgcgag cgtcggccgc
     5041 ctcgatcgat gagaacgaga acgtggcgcg cccgttctgc gtgtagctcg tgtcgaagaa
     5101 gtcgagatcc ccgttgtcgt cctgcgacac gttctcgagg taggcctcgg gtgtcgtgac
     5161 cgcgccgtag atcgtcggct cgtcctcctg cgagagcgcg aacgtcttcg cgaagcagcc
     5221 ggcctcggtg gcgtacacct tgccgcccgg catcatcgcg acgaagtcgt cctgcaccgg
     5281 cagcgaaccg ttctggcgcg tgaacgtggt ggtggtctta ccggtgcccg agagcccgac
     5341 gatcaggccg accttgtcgc cgtcgtcggt ggggatcacc ttgcagcccg cgtgcagtgg
     5401 gagcccgccg cggtcgaaca cgagcttgtt ccacatccgc aggccgccct tcttcgactc
     5461 gccgaagtag tcggagttgc agacgcgggt cacgccgcgc tccaggtcga cggcgatcac
     5521 gcggtcgttc ggatacccct cgacggcgag gtggggcgtg tagatcacgg tgagctccgg
     5581 ctggaagcca tcgccgtcgt cgacgtcgaa gtacagctgc ttctgcatgc ccgcgatgtt
     5641 cgcgttgctc tcctcgatgt acaggcgagc gggggtgcgg aactcggggt cgttgccgat
     5701 gtagccgtcg acgatgacca tgtcgcgccc ggcgatgtac tcgtcctgga ggcgcgcgat
     5761 gcgctcgccc tcctcgcgcg agatcgcctg ctgcgagctc ccgtccggat catctaggac
     5821 caggaacgtg gaggccttcg agcgcgcgag cacctcggtc tgcacgttga ggttgtcgta
     5881 cttcgttcga cgcgcattgg gcatcttggc cgcgagctcc ttcagctcgg cggccgaggg
     5941 attgacgcgg accgactttg cgtcggggag aggagatagc tgcttagcca ttgtgtgagt
     6001 gcgtccgctc ctttgagggt tggcaactgt aaccaccaac gcgggacgag agcggcggca
     6061 ttcggggccc gcggaaagcc caggtacccc gtttgggtca ccggcaagcg gaatttcgct
     6121 agggcacgag gctctcgacg tccgagccgt ccagcacgcg caggaaagcg cgcacgacct
     6181 cggagtcgaa ctgggagccc gagcaggcga gcagctcggc gcgcgcctcg tccgttgtga
     6241 gcgcccggcg gtagggccgg tcggacgtca tcgcctccca ggcgtccgcc acagagagga
     6301 tccgcgcctc catcgggatc tcctcgcccg cgagtccgtg cgggtagccc gtgccgtcga
     6361 agcgctcgtg gtgcgcgagc acccaggcac ggatgtcctc gatcgacccg gagtcgagga
     6421 tgcgcgccgc gatcagcggg tggcgcttga gctcctccca ctcctcctcg gaaagcggcc
     6481 cgttcttgga gagaatgctc tcgggcacgc cgatcttgcc gacgtcgtgc agcacgccgc
     6541 cgattcgcac acggtcgacc acctcttccc cgagcccgag ctccctcgcg atcagctccg
     6601 cgtagcgggc cacgcgccgt gagtgggtgg cgctgccgct ctcgcgagcc tcgagcgcct
     6661 cggcgagcgc cagcaccgtc gccagatagc cgtcgcgctc gaaacggcgc cgcgtgccag
     6721 cgatcgcgcc cgcgagctcc gggttgtgga tcacggtgcg gtcgcggccg agctccttgg
     6781 cggcgtagag cgcgaggtcg ccggcgtgaa agagcgcctc tgcggtacgg ccatgcttgg
     6841 ggaagctcgc gacgccgaag ctgatcgtca cgcgccggtc gctgtcctcg aactgctcgc
     6901 gcacggcgcg gcggaggcgc tccgcgagca cgtaggcgtc gtgctcctcg gtctccggca
     6961 cgatcagcgc gaactcctcg ccgcccacgc gggccgccgt gtcgatgcgg cgcttggcgg
     7021 cgcgcaggac cgaggcgagc cgctcgagcg catcgtcgcc ggcgcggtgg ccgaagcgat
     7081 cgttcagatc cttgaagtgg tcgatgtcgc cgacgatcag gctcagcggc cggttcgagc
     7141 gcgtcgcacg ctcgagctcg ttgccgaaca gctcctggaa gccgcggcgg ttcagcagcc
     7201 cggtgagcac gtcggtgcgc gccgcgtccg agaggcggcc gatgaggcgc tcgagccggt
     7261 cgcgcatcgt gcgaacgagc gctgcggtcg tagcgagcgt gccgaccgtg agcagccagg
     7321 ccgcgaccga gccgtggtcc gcatccgagg cgagcagcac caccgcgtag gaagcggcgg
     7381 cgacccccac ctgtacggcc gcctccctcg gcgtgaagaa ataggcggca tagagcgcga
     7441 tcgggacgta gagcatcggg taggtgctcg tcgcgttgtt gctccagtag atcgccgccg
     7501 acacgagcag cgttccgcag gcgagcacca ggtgaaaggc ccaggcgcgc agccgcttcg
     7561 cccctagtac cagcacggcc agcagcgcgg cggtcgcgac cgcggcgagg gctccgagcg
     7621 gagcggcgac accgacgttc ggcacgtcga ccgagctcgg cagcgcgagc gagacgaccg
     7681 ccagcgtcga ccccacggca aacacgtagg cgagcgtcct tgccatcacc gagcggtcgg
     7741 cggccgccgc ccgggcttcc tcaccgaggg acggcgcgcc ggcctgcgcg tccagcgcag
     7801 tcatgggtcg aatatcgacg gcgcgcaaaa gcgccgcaac tcggccattc gtccaagcga
     7861 cccacgattc gccgcacatt gcggacatac acttcctcgc gtggtgaggc agcgctccac
     7921 ccacgtgcgc gccgtggtgc gcttccttgc tgctgtcgtg gcggtcgccg gggccctgct
     7981 gattgccgac gctgtgatca ccgtcgcgtg gcaggaaccg atctcggcga tgatcgcgag
     8041 ccgcgagcag gaccggctcg agcaccagct cggtgcactc gagacgcagt ccgccgccga
     8101 cgctcgttcg gtcaacgtgc gcggcctgcg ccaccgcgtc gccgtgctcg ccgcgcgcgc
     8161 acgcgcgcgc gcgaagacgg gccacgcgat cggcacgatc cgcctcccga cgctccaccg
     8221 ccactacgtg atggtgcagg gcacggacac cggcagcttg cgaagggggc ccggccacta
     8281 ccccgcgacg ccgttccccg gccagcgcgg aaccaccgct gtggccggcc accgcaccac
     8341 ctacctcgcg cctttcagga cgatcgacca gctgaagaag ggcaaccgca tcgtcgccga
     8401 catgccctat gggcagatca cgtacgaggt cgaatcgacg cgcatcgtca agccgaccgc
     8461 gctctgggtc actcgcaagg tcgaccacga caggctcgtg ctgagcgcct gccatccgct
     8521 ctacagcgcg tcgcaccgga tcgtggtgtt cgcgcggctg gtgtcggtcg caccacccgc
     8581 ctagaggcga agcagcggca gcacgccgcc gctcagcccg ctctggtcgc gcgactcgaa
     8641 gaacagcacg agcgctacga gcagcgccag ggcgagaatc accacggcca cccagtaggc
     8701 caccttggc
//
LOCUS       DASVXS010000056         8141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_296680, whole genome shotgun sequence.
ACCESSION   DASVXS010000056 DASVXS010000000
VERSION     DASVXS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296680"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VF752_03050"
     CDS             complement(<1..690)
                     /locus_tag="VF752_03050"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8854551.1"
                     /translation="MPETRSDQVDISLVIPAFNERENLPVLVDACRQVLSPYEGSHEI
                     VLVDDGSSDGTRELLDELARDEPLLRPVHHDVGANIGCHPSELEGLKLSRGDVMVFLP
                     ADLQIHPEVVPQFVDAAQQADIVASHRVQRADAAWRRYLSGANNWFERLLMGVDVHDA
                     HSSMALTRRAVDEVIPKVVSNSALIPAEVLVRADRMGLPITEIEIEHHPRAAGVQTGA
                     KPSEILKVQLDL"
     gene            787..2574
                     /gene="asnB"
                     /locus_tag="VF752_03055"
     CDS             787..2574
                     /gene="asnB"
                     /locus_tag="VF752_03055"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8854552.1"
                     /translation="MCGICGIYAPGRALDPAEADIASMLSAISHRGPDASGVHHAPNL
                     SLGSARLAIIDLVRGNQPLYSEDRQVVAVYNGEIYNYRELQRELRGRGHKLASESDSE
                     VLTHLFEEQGISFVERLNGIFGFTLWSEHERALYIARDRFGVKPVYYHWDGETLLFAS
                     EAKSILASGRVRPVLDREAFLELLTFQNVLSHRSLFDGIKLLPPASTLRLDESGLRID
                     TWWDPLPNPQPARDRDQLPHLVGEQFEAAVERQLVSDVELASYLSGGLDTGAVTAAAA
                     PRLDRLTTFCTGFDTTGAEGMEAEFDERRDARELAQHLGTHHHELLLDPSDMEMVLPR
                     LVHRLEVPRMSFSYPNYLTAGMTSRWVKVVLSGAGGDELFGGYPWRYEFAERDDFEGA
                     YFGYWERLLRRDDMAGALAPGFARDVDLDRPRKVYDEFMAGGAQLSPLDRILYFESKT
                     FLHGLLVLEDKLSMAHSLEARVPFLDNELVDFILTIPAQEKLQSGRSKDLFREAMRGR
                     LPDNVRERKKTGFTPPQATWFQTSQREYVREVLLSERALERGIFRPGFIESVLDDHEK
                     GRSDRRLLIWTLVCMEWWHRVFIDGEHAL"
     gene            2571..3656
                     /locus_tag="VF752_03060"
     CDS             2571..3656
                     /locus_tag="VF752_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8854553.1"
                     /translation="MSKQDIRIGLAGVGYWGSKLARNIYDARGCTLAAISDAEAPRME
                     AAVERYAGSRGIDSLEQMISDPELDAVVLATPASMHAEHARAALEAGKHVLVEKPLAL
                     TSRDCDELCELAASQERVLMVGHTFIYSEPVRMLRWMIENGELGDLLYVYGQRLNLGV
                     IREDLNALWNFGPHDISILLYLLDDVPTRVSARQFSLLNRKLEDVAFLVLEFSNGVVG
                     HIHDSWLDPRKVREFTVVGQSKMAVYDDTDVESPLRVYDKGVVPMPFGEDETRRFSSL
                     VQSESDGYGEFKLEVRAGDMLAPRVNGREPLRVEIEHFADCAASGTTPLTDGVHGGKV
                     VRILEAADRSAREGGVAVDIELSRQVA"
     gene            3653..4774
                     /locus_tag="VF752_03065"
     CDS             3653..4774
                     /locus_tag="VF752_03065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8854554.1"
                     /translation="MNIPLLDIHAQHDSIREELNDAIRSVVESGRYIGGAPLSDFESS
                     FARFCGAKRAIGVSSGTDAVTLALRGCGVGPGDEVITTAFTFIATVESIAEVGANPVL
                     VDVDPDTALATVDVVEDAISDKTAAILVVHLYGQPVDLPAFRELAERRGLALIEDAAQ
                     AHGAEFDGLRVGAVGRAAAFSFFPGKNLGALGDAGGITTGDGALADRIAALRDHGRLD
                     KYRHEELGVNARMDTLQAAILAVKLKRLGEWNEARRRHATFYEEAFADVDGIEPLTVD
                     ERATHVYHQYVVRVDDRDGAVAALKAAGVAAGVHYPIPLHRQPALAESHGYLSLPACE
                     SLAERVLSLPVYPELTDEQRSHVAQAVTDHVRSRSGVAS"
     gene            4771..5889
                     /locus_tag="VF752_03070"
     CDS             4771..5889
                     /locus_tag="VF752_03070"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8854555.1"
                     /translation="MKTLVTGGAGLIGSWVVDLALEKGDDVRILDNLDSQTHPKGRPD
                     WIPADAEFIEGDVRDADTLARALDGVDEVYHQAAFGGFTTEISTYYDTNATGTSLIFE
                     TIAERGLDVRKVVAASSQAIYGEGFYRCAEHGTVQPGMRTLEQFEQGRWEPYCPECGR
                     EVTPLLTPETAGWNGETPYAVSKLAEERTVIGMGKRLGIPTAALRYAVTFGPRQSVFN
                     PYTGIVSIFSTLLLNGLKPNVYEDGHQTRDYIFVRDIARANLLVMEDYRANGRVFNVG
                     RGVPVTVVELVEKIAAAYGLEADFEMRGDFRPGDVRHLVHDATAIRELGWEPGSTLEE
                     GLEAVVEWVRSLGEVREYFSDALERLRRTGVVMSSSAS"
     gene            5889..6875
                     /locus_tag="VF752_03075"
     CDS             5889..6875
                     /locus_tag="VF752_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8854556.1"
                     /translation="MLESNETATRTLDLLRCPSCGGRLSEQGADKLECESGHTYPVVR
                     DIPRLFDKARLSLEQSRTADAFGYSWTHYPKENPYTEEQWRDWVEPLTREDFDGRLVL
                     DAGCGLCGFAEFALDWGAREVVGIDLSQAIDAARDRLGDRVGLVQGDLNEMPFEPGTF
                     DIAYSIGVLHHLPDPERGFQSLARMARPGGKVFAWVYGRENNGWIVHIVDPLRRHVFS
                     RLPRAFVKWGISLPVVGLLWPAIKLAGRLKKIPYGDYLRWLGERDLEFVHGVVFDHLV
                     APTSHYIRREEFEEWFRRGGLEDVKISWRNQNSWRGLGTVPGGPPDALPADG"
     gene            6919..7980
                     /locus_tag="VF752_03080"
     CDS             6919..7980
                     /locus_tag="VF752_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8854557.1"
                     /translation="MEARETVMEPRAAMSHAPAAAAGRINGKRITLVLLGIVVSVFFG
                     WLATRKISLDEVRRSLTDAHYGWVIPSLVLTFVGGWLRAIRWKLLFRDPERVTVTESF
                     GALSIGLMFNNLLPSRAGEVPRLFALRRTTGLSAFEIGTTIITERILDVFVLALAAVA
                     LLPLFPNRGWIHLLGVICAGIVAGCVLLAVLVAVFRQRLRSVLLWGLRKLPFLGEERA
                     RSIVEGVSAGGRILMRPRRLAVTLALTALAWGAVGLSGWVLFPAFDLHPGAAAPWLIL
                     VANSFALTIPSSSAAVGLYEASVQASLVAFGVSHSTALSYALVLHAINFFPIILVGIA
                     ASWLMSRSAPHPRLRSSPT"
BASE COUNT         1238 a         2819 c         2769 g         1315 t
ORIGIN      
        1 cagatcgagc tgcaccttga ggatctcgct cggctttgca cccgtctgca cgccggcggc
       61 acgtgggtgg tgctctatct cgatctctgt gatgggcagg cccatccggt ccgcgcgcac
      121 cagcacctcg gccgggatca gcgcgctgtt cgagacgacc ttcgggatca cctcgtccac
      181 cgcgcgacgg gtgagcgcca tgctcgagtg agcatcgtgc acgtccacgc ccatgagcag
      241 ccgctcgaac cagttgttcg cgccgctcag gtagcgccgc caggcggcgt ccgcccgctg
      301 tacgcggtgg ctcgccacga tgtcggcctg ctgcgccgcg tccacgaact gcggcactac
      361 ctcggggtgg atctggagat ccgccggtag gaagaccatg acgtcgccgc gcgagagctt
      421 cagaccctcg agctcggacg gatggcagcc gatgtttgcg ccgacatcgt ggtgcaccgg
      481 ccgcaacaag ggctcgtcgc gcgccagctc gtcgaggagc tcgcgggtgc cgtcactcga
      541 gccgtcgtcg acgagcacga tctcgtgtga gccctcgtac gggctgagga cctggcggca
      601 cgcgtccacg agcactggaa gattctctct ctcgttgaat gcggggatca cgagcgagat
      661 gtcgacctgg tcgctgcgcg tttcgggcat cgtcgaggac ccggaaagtc ggttgctaag
      721 ctcccccgcc gccattcggc ggccgattct agagcgctat cgcggcggcc cgtcgagttc
      781 tctcaaatgt gcggaatctg cggcatatac gcgccaggcc gcgcgctcga cccggccgag
      841 gcggacatcg cctcgatgct gtcggcgatc tcgcaccggg ggcccgatgc cagcggcgtg
      901 caccacgcgc cgaacctctc gcttggctcc gcccgcctag cgattatcga cctcgtgcgc
      961 ggcaaccagc cgctctacag cgaggaccgg caggtggtcg ccgtctacaa cggcgagatc
     1021 tacaactacc gggagctcca gcgcgagctg cgcggccgcg gccacaagct cgccagcgag
     1081 agcgactccg aggtgctcac ccacctgttc gaagagcagg gcatctcttt cgtcgagcgc
     1141 ctgaacggca tcttcggctt cacgctctgg agtgaacacg agcgcgcgct ctacatcgcg
     1201 cgcgaccgct tcggcgtcaa gcccgtgtac taccactggg acggcgagac gttgctcttc
     1261 gcgtccgagg cgaagtcgat cctcgcgagt ggtcgcgttc gcccggtgct cgaccgcgag
     1321 gcgtttctcg agctgctcac attccagaac gtgctctcgc accgctcgct cttcgacggc
     1381 atcaagctcc tccccccggc gagcacgctg cgactcgacg agtcggggct ccgcatcgac
     1441 acctggtggg atccgctccc caacccgcaa ccggcgcgcg accgcgacca gcttccgcat
     1501 ttggtcggcg agcagttcga ggccgccgtc gagcgccagc tcgtgagcga cgtggagctc
     1561 gcgagctacc tgagcggcgg cctcgacacg ggcgccgtca cagcggcggc tgcgccgaga
     1621 ctcgatcggc tgacgacctt ctgcaccggc ttcgacacca ccggcgccga ggggatggaa
     1681 gccgagttcg acgagcgccg cgacgcgcgc gagctggccc agcacctcgg cacgcaccac
     1741 catgagctgc tgctcgaccc gagcgacatg gagatggtgc tgccgcgcct ggtgcaccgc
     1801 ctcgaggtcc cgcgcatgag cttctcctac ccgaactacc tgacagccgg gatgaccagc
     1861 cgctgggtca aggtcgtcct ctcgggggca gggggcgacg agctgtttgg cggctacccc
     1921 tggcgctacg aatttgctga acgcgatgac ttcgagggcg cctacttcgg ctactgggag
     1981 cgccttctca ggcgcgacga catggccggg gcgctcgcgc cgggcttcgc gcgcgatgtc
     2041 gacctcgacc ggccgcggaa agtctacgac gagttcatgg cgggaggagc gcaactctcg
     2101 ccgctcgacc ggattttgta cttcgaatcc aagacgttcc tccatgggct gctcgtgctt
     2161 gaggacaagc tgagcatggc tcacagcctc gaggcgcgcg tgccgttcct ggacaacgag
     2221 ctcgtcgact tcatactcac gattccggcg caggagaagc tgcaatccgg gcgctcgaag
     2281 gacctgttcc gcgaggctat gcgcgggagg cttcctgata acgtccgcga gcggaaaaag
     2341 acgggcttca cgccgcctca ggcgacgtgg ttccagacga gccagcggga atacgtgcgg
     2401 gaggtactcc tctcggagcg ggctttggaa cggggaatct tccggcccgg cttcatcgag
     2461 tccgtcttgg acgatcacga aaagggcagg tccgaccgcc gcctactgat ctggacgctc
     2521 gtctgcatgg agtggtggca ccgggttttc atcgacgggg agcacgcgct atgagcaagc
     2581 aggacatacg tatcggcctg gcgggcgtgg gctactgggg gtcgaagctc gcgcggaaca
     2641 tctacgacgc gcgcggctgc accctcgccg ctatctccga cgccgaggcg ccccgcatgg
     2701 aggccgccgt ggagcgctac gccggctccc gggggatcga ttcgctcgag cagatgatct
     2761 ccgacccaga gctcgacgcg gtggtgctcg ccacgcccgc gtcgatgcac gccgagcacg
     2821 cccgcgctgc gctcgaggcc ggcaagcacg tgctggtcga gaagccgctc gcgctcacca
     2881 gccgagattg cgacgagctc tgcgagctcg ccgcatccca agagcgcgtc ctgatggtgg
     2941 gccacacgtt catctactcc gagcccgtcc gcatgctgcg ctggatgatc gagaatggcg
     3001 agctcggcga cctgctctac gtgtacggcc agcgcctcaa cctcggcgtg atccgcgaag
     3061 acctcaacgc gctgtggaac ttcggcccgc acgacatctc gatcctgctc tacctgctcg
     3121 acgacgtgcc aacgcgggtc agcgcccgcc agttctcgct gctcaaccgc aagctcgagg
     3181 acgtggcgtt cctggtgctc gagttctcga atggtgtcgt cggccacatc cacgactcat
     3241 ggctcgaccc gcgcaaggtc cgcgagttca cggtcgtcgg tcagagcaag atggcggtct
     3301 acgacgacac cgacgtggag agcccgctgc gcgtgtacga caagggcgtc gtgccgatgc
     3361 cattcggcga ggacgagacg cgccgcttct cgtcgctcgt gcaatcggag agcgacggct
     3421 acggggagtt caagctcgag gtgcgcgccg gcgacatgct cgcgccgcgc gtgaatgggc
     3481 gcgagccgct gcgcgtcgag atcgagcact tcgccgactg cgccgccagc ggcacgacgc
     3541 cgctcaccga cggcgtgcac ggcggcaagg tcgtgcgcat cctcgaggcg gccgatcgct
     3601 cggcgcgcga gggcggcgtg gccgtggaca tcgaactcag taggcaggtt gcatgaacat
     3661 cccccttctg gacatccacg cccagcacga ctcgatccgt gaggagctga acgacgcgat
     3721 ccgctcggtc gtggagagtg gccgctacat cggcggcgcg ccgctcagcg acttcgagtc
     3781 gtccttcgct cgcttctgcg gcgctaagcg cgccatcggc gtgtcgagcg gcaccgatgc
     3841 cgtcacgctc gcgctgcgcg gctgcggagt cggccccggc gacgaggtca tcacgaccgc
     3901 cttcaccttc atcgcgacgg tcgagtcgat cgccgaggtg ggcgcaaatc cggtgctcgt
     3961 ggacgtggat cccgacactg cgctggcgac cgtcgacgtc gtagaggatg cgatcagcga
     4021 caagaccgcc gcgatcctag tcgtgcacct gtacggccag ccggtcgacc tccccgcttt
     4081 ccgcgagctg gccgagcgcc gcggcctcgc gctgatcgag gacgccgcgc aggcccacgg
     4141 cgcagagttt gacggtctgc gagtaggcgc tgtcggccgc gcggccgcgt tcagcttctt
     4201 ccccggcaag aacctcggtg cgctcggcga cgcgggcggt atcacgacgg gcgacggcgc
     4261 gctcgccgac cggatcgccg cgctgcgcga ccacgggcgc cttgacaagt accggcacga
     4321 ggagctcggc gtgaacgccc gcatggacac gctgcaggcg gcgatcctgg cggtgaagct
     4381 gaagcgcctg ggcgagtgga acgaggcgcg ccgccgccac gccaccttct acgaggaggc
     4441 gttcgcggac gtagatggga tcgagccgct gacggtggac gagcgcgcga cccacgtcta
     4501 ccaccagtac gtagtgcggg tggacgaccg cgacggcgcg gtagccgcgc tgaaggcggc
     4561 cggcgtggcc gcgggcgtgc actacccgat cccgctccac cgccaaccgg cgctcgccga
     4621 gagccacggg tacctgagcc tgccggcctg cgagtcgctg gccgagcgcg tgctttcgct
     4681 gccggtctac cccgagctca ccgacgagca gcgctcgcac gtggcgcagg cggtgaccga
     4741 ccacgtgcgc tcgcgctcgg gggtggcttc gtgaagacgc tcgtgaccgg cggcgccggg
     4801 ctgatcggct cgtgggtcgt ggacctggcg ctcgagaagg gcgacgacgt ccgcatcctc
     4861 gacaatctcg actcgcagac gcatccgaag ggccgccccg actggattcc cgcggacgcg
     4921 gagttcatcg agggcgacgt tcgcgacgcc gacacgctcg cgcgcgcgct cgacggcgtg
     4981 gacgaggtct accatcaggc cgccttcggc gggttcacga ccgagatctc gacctactac
     5041 gacacgaacg cgaccggcac ttcgctcatc ttcgagacga tcgccgagcg cggcctcgac
     5101 gtgcgcaagg tggtggccgc ctcgtcgcag gcgatctacg gcgagggctt ctaccgctgc
     5161 gccgagcacg gcaccgtcca gccgggcatg cgcacgctcg agcagttcga gcagggtcgc
     5221 tgggagccgt actgcccgga gtgcggccgc gaggtcacgc cactcctgac ccccgagacc
     5281 gccggctgga acggtgagac gccctacgcc gtgtcgaagc tcgccgagga gcgcaccgtg
     5341 ataggcatgg gcaagcgcct gggcatcccg acggcggcgc tccgctacgc ggtcacgttc
     5401 ggcccgcgcc agtcagtgtt caacccgtac accggcatcg tctcgatctt ctcgacgctg
     5461 ctgctgaacg gcctcaaacc gaacgtgtac gaggacggcc atcagacgcg cgactacatc
     5521 ttcgtgcgcg acatcgcgcg cgcgaacctg ctcgtcatgg aggactaccg cgccaacggc
     5581 cgcgtattca acgtgggccg gggcgtgccg gtcacggtgg tcgagctcgt ggagaagatc
     5641 gccgcggcgt acggcctcga ggccgacttc gaaatgcgcg gcgactttcg ccccggggat
     5701 gtgcgccatc tggtgcacga cgcgaccgcc atccgagagc tcggctggga acccgggtcc
     5761 acgctcgagg agggcctcga ggcggtggtc gagtgggtcc gcagcctcgg cgaggtgcgc
     5821 gagtacttca gcgacgcgct cgagcgcctg cgccgcaccg gtgtcgtcat gtcgagctcc
     5881 gcgagctgat gctcgagtcc aacgagaccg caacgcgcac gctcgacctg ctgcgctgcc
     5941 cgtcctgcgg cggacgactg agcgagcagg gcgcagacaa gctcgaatgc gagtccggcc
     6001 acacctatcc ggtcgtgcgc gacatcccgc ggctgttcga caaggcgcgg ctctcactcg
     6061 agcagtcccg gacggcggac gcgttcggct actcgtggac gcactaccca aaggagaacc
     6121 cgtacaccga ggagcagtgg cgcgactggg tcgagccgct cacgcgcgag gacttcgacg
     6181 gccggctcgt gctcgacgcg ggctgcgggc tgtgcgggtt cgccgagttc gccctcgact
     6241 ggggggcgcg cgaggtggtg ggcatcgacc tctcacaggc gatcgacgcc gcgcgcgacc
     6301 gcctcggcga ccgcgtcgga ctcgtgcagg gcgacctcaa cgagatgccg ttcgagccgg
     6361 gcaccttcga catcgcctac tccatcggcg tgctccacca tctgccggac cccgagcgcg
     6421 gcttccagtc gctcgcgcgt atggcgcggc cgggcggcaa ggtgttcgcg tgggtttacg
     6481 ggcgtgagaa caacgggtgg atcgtccaca tcgtcgaccc gctccgtagg catgtgttca
     6541 gccggctgcc gcgcgcgttc gtgaagtggg gcatctcgct gccggtcgtc ggcctgcttt
     6601 ggccggcgat caagcttgcg gggcgcctca agaagatccc gtacggcgat tacctgcgct
     6661 ggctcggcga gcgcgacctc gagttcgtgc acggcgtggt gttcgaccac ctggtcgccc
     6721 ccaccagcca ctacatccgc cgcgaggagt tcgaggagtg gttccggcgc ggcggtctcg
     6781 aagacgtgaa gatcagctgg cgcaaccaga actcgtggcg agggctgggc acagtgcccg
     6841 gaggaccgcc tgatgcgctt ccagcggacg gctagcctgt ccagccgagg cgccccgggc
     6901 acggagtcct ccgctgtgat ggaggcacgc gagacagtca tggagccgcg cgcggcgatg
     6961 agccatgcgc cggcggcggc cgccgggcgc atcaacggta agcgcatcac gctcgtgctg
     7021 ctcggcatcg tcgtgtcggt ctttttcggc tggctcgcca cgcgcaagat ctcgctcgac
     7081 gaagttcgcc gtagcctcac cgacgcgcac tacggctggg tgatcccgtc gctcgtgctc
     7141 accttcgtgg ggggctggct ccgggcgatc cgctggaagc tgctgttccg ggatcccgag
     7201 cgcgtgaccg tgaccgagag cttcggcgcg ctcagcatcg gcttgatgtt caacaacctc
     7261 ctgccttcgc gcgcgggtga ggtgccgcgc ctgttcgcgc tgcgccggac gaccgggctg
     7321 tccgcgtttg agatcgggac gacgattatc acggagcgca tcctcgacgt gttcgtgctc
     7381 gcacttgcag ctgtcgcgct cctgccgctg tttccgaacc gaggctggat ccacctgctc
     7441 ggcgtgatct gcgcaggcat cgtcgcgggt tgcgtgctgc tggcagtact cgtcgcggtg
     7501 ttccggcagc ggctgcgcag cgtgctcctc tgggggctgc gaaagctccc gttcctgggc
     7561 gaggagcgcg cacggtcgat cgtggaaggc gtatcggctg gcggccgcat cctcatgcgc
     7621 ccgcgccggc tcgctgtgac cctggcgctt accgcgctgg cttggggcgc cgtgggcctc
     7681 tcaggctggg tgctgttccc ggcattcgac ctgcatcccg gcgcggcggc gccgtggctg
     7741 atcctggtgg cgaacagctt cgccctcacg atcccgtcga gctcggcggc cgtcggcctc
     7801 tacgaggcct ctgtgcaggc gtcgctcgtg gcattcggcg tctcccacag cacagccctt
     7861 tcgtacgccc tcgtgctgca cgcaatcaac ttcttcccga tcatcctcgt gggcatcgcg
     7921 gcctcgtggc tgatgagccg atccgcgccg cacccgcggc tgcgaagctc ccctacgtga
     7981 gcacggccgg agtccaggag agtccagcca ccacgccggc gcacgcgccc actcggcgcg
     8041 gccgaggcgc cctgctactc accacctcgg ctccgacctg gcgcgagttc gcggtcgcga
     8101 gcgtgctgat cggcgtagcc gcgatcgcct tctacgccga g
//
LOCUS       DASVXS010000057        29447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_998201, whole genome shotgun sequence.
ACCESSION   DASVXS010000057 DASVXS010000000
VERSION     DASVXS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29447
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998201"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF752_03085"
     CDS             complement(<1..849)
                     /locus_tag="VF752_03085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854558.1"
                     /translation="MSAREPAVTVLTPVFDGEAHLAEALDSVASQTFEDWECVVVDDG
                     SRDASAEIARRFAAADDRFRVHSQENRGQPYALNAGLGLARGRYVAILDADDVALPDR
                     LERQVGRLESDERLGLVGGAVVMIAGDGRDLGLAEYPLGDGEIRQRLEERTAFVHSAA
                     TFRRELAIAAGGYRPAARLAEDLDLWLRLAERAALANLADPVVRYRMHPGQLSITRIE
                     EQVMSVQAGLAAARERRAGRPDPLVGVERLDRELLHSLGVDDAAIARMAAETAAWWGR
                     VLERAGD"
     gene            complement(846..2447)
                     /locus_tag="VF752_03090"
     CDS             complement(846..2447)
                     /locus_tag="VF752_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX8854559.1"
                     /translation="MSTTASSSGPEPPSSDRPAAGAAATATTAASVTRGGLWVALARV
                     LPQLYTLGISVAAARYLGPDGMGRQSFIAFVEVSAASIFTGGLNVALMRYVGETLGRD
                     RPEVVRGLVTWAWKLVILGGVPAAGVLVAIGLSGSEPQAAWYLAGLVCFLLILATVPS
                     AALAGAQRWRLSTRPGVVTGAVSVPATIAVLAAGGGITGLFAVEAAVVGANLLWTSLL
                     ARRVLAELSDAHSEPIGELKRDVLGYTGLMAGGVVLTFVIWKRSEFVFLDHYSGDTQI
                     AFYSIAFAAVGALGLLPGVVSSVISPAFATLYGAGHADRLRSGYGRSIRLLLLVSLPV
                     TAGALAVGPATVQLVYGSDYAGVRTPLLIMLAVFPLVALSGGANALMVGLGRRLVPGV
                     VGVAAAVVTLGLNWLLVRSHGATGAALANSGGQVAATVPIVVYAVTSIGGLEWGVGSI
                     LRMALASAVTWLAAHLAVVAVHGGWEVPAGVAAGLLAFAVCAPPLRVLPAEEAELVEQ
                     VAGERLRPLARRLASVLSHGYAAAR"
     gene            2630..3655
                     /locus_tag="VF752_03095"
     CDS             2630..3655
                     /locus_tag="VF752_03095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854560.1"
                     /translation="MRRFVAPPPRGAPAAAGEPSFSVLIPAYQAESTVAETVESALAQ
                     TYPAQEVIVCDDGSTDGTAAALEPYRDRIVYLRKENGGGASALNHAAARASGDFLVIL
                     DSDDVYLPERLEAIAELARERPDVGMLMSDAWFEVEGERVGRFAQENGFEVADQRRGI
                     LERCFIFAPAVSRESVAAIGGWDEQLAIGYDWSCWMRLIFSGVRAGLVDEPLMHYRLR
                     EGSLASSRVEALRDRVRVLDRVSEQLELSQHERELFTSARHVHERRLKLADAELGLSD
                     GAPDVRSRLRAVATDDGFDAPTRAKALAAMAAPGVARRLVQRRASQGRTRLVRTSPTP
                     GGATRDD"
     gene            3648..5270
                     /locus_tag="VF752_03100"
     CDS             3648..5270
                     /locus_tag="VF752_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8854561.1"
                     /translation="MTEGLQPPRTVVLPEGAPPPGADAVDIKGDGAFDLPGTTSGATV
                     MRGGIWTAVSRMIPQVYTLIISLVAARFLGPDGMGRQSFIAFVELSTMQLFTGGLSLG
                     MMRNIGEALGAGRAGAVRAIISWGWRLQAIGCVGGVLVLGGAAAFGAEPTAAWVLAAV
                     ACFMGVMHTVPSAVLIGMQRWRDASIVGLVTGSIAVPATIAVLAAGGGIAGIFAVEAA
                     TTTMNLIWTTRLARRSLDGLGDGNHDPGSDYKRQIVRYTLATTVTMVLGLIVYRRSEF
                     FFLEHYSTNSEIAFYSIAFSAMSAVVLLPEAFGGALAPAIATLVGAGDHERIRSGYSR
                     ALRLAIMAALPLTAAIMTLVPPLIELVYGRAYADTRGILIIMAIIFPILPLLNMGNGL
                     MLGLGKMRVPVIASFVAAFVNVGLDFALIPDHAAKGAAVANAAAQTTVAVILVLYIWR
                     LLGGIVLRPLPIVLSALASAAAAAAALAVLAAAGTGVPGVIAASVAFVLALLGAGRIL
                     RFLPSDDAAWLTENIRGRGRKPLARLSRLLSG"
     gene            5267..6265
                     /locus_tag="VF752_03105"
     CDS             5267..6265
                     /locus_tag="VF752_03105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854562.1"
                     /translation="MRPFLAPAAKGEPEAAVQPSFSVAIAAYQAEAIVGDAIESALAQ
                     TVPAVEVIVCDDGSTDGTGAAIERFGSRVTHIRKENGGEASAKNAAARAARGDFVVFL
                     DADDLFEPERLAALGELAAHRPDLDILTTDALLELDGRVARRCYDASFPFEVDDQRRA
                     ILERNFLFGLAAVRRSRLREAGGFDEEIPYATDWDLWLRLIMSGARAGMVDEPLARYR
                     ISGGLSSHRARLFEGRARVIAKALEREHLEPHERRAAEHALAEQRKRAALEGAREALV
                     ERRPDARRRSLDVALGPGHTLPSRLKALVSAAAPGLAGRRLADRPRETTAGLLLPD"
     gene            complement(6168..7385)
                     /locus_tag="VF752_03110"
     CDS             complement(6168..7385)
                     /locus_tag="VF752_03110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8854563.1"
                     /translation="MARVSAPPELLEDAELGLAEGAARPEAAAVRLRRRGVELSWGEP
                     VPLDRRALLRAARERGRSSVAMLRADPSLVTEMQVGSWFNAGWRARALRRVVLRTRTE
                     RLLAPLGGLARRTAIDAAFWAGVRDAADADEWRRLARSSYTALCYHRLAGDMKAGQEA
                     MDMPPRLFRRQLRVLRLLGFRPLSAEELQRFHSDARSWLPRRRYLLTADDGFSDNLTP
                     LRGAEAAQLFVPTREVGRGAWWMGGEPIASWEELAQLERDGVAIGSHSGSHVALTDLP
                     EAELRGDLAASRRELAEHLSSPLALLAYPHGRHDERVRSAAIGAGFGGAYTTLNGRNG
                     AGTDAFCLRRVEPKAHDGVAGFLWKVLTGELLPRRWERRVNPAAAGRRSSPAAGPRDD
                     DPQAPAPPPTPVP"
     gene            7490..8593
                     /locus_tag="VF752_03115"
     CDS             7490..8593
                     /locus_tag="VF752_03115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8854564.1"
                     /translation="MDQPTRGNSAGPRRRLMIYSDALNVGGAEVSVGHLIAELPERFE
                     VTIAGVQPDVVQSIAARRPGTNVEVLDRVRDKLDVVAIADHVRLLRRARPDILHVNLF
                     TPWSGQYAQLAGVATPGVRTVAVEHLPMPATHRRQRFAKRALSRAVSAHVAVGERSAR
                     EIEQLVGLPTESIMTIYNGVPQNAPPPLERRADGPVVGSVGRLSEQKGYDLLVRALPD
                     LPDAQLVLVGDGPERARLESLARELGVSERLRITGWSDKVSSHLAYFDVFALPSRYEA
                     FPLAILEAMFAAVPVVAAEVGSVPEAVQPGVTGLTVAPGSASELASAIRSLLADPERA
                     RELGRNARRMALDRFSVRAMTDRYIALYDRLAG"
     gene            8670..10133
                     /locus_tag="VF752_03120"
     CDS             8670..10133
                     /locus_tag="VF752_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854565.1"
                     /translation="MSAALTDARFALPTPVRTAAVIGGLSGWAEPLAAAGIEVSASLD
                     GGPAPDLVVAPGRLARAAVTSGAAGVIVHGADASAVASAEGMTGHRLLALPRLHEPVA
                     FLALGNPDLARYALQEMLVPTRRWKEVRNRALLPVLSRGLVPSRTQVTVASHRRGDPF
                     LVAAARQLGIPEQATWLLSPGQGDVLARGAFHLFPEGSDHPSHVLKFSRIPGRSQPFD
                     RDEHGLAVAEAGGPTVAPHAPRLLGRFEEAGMHASVETAARGRQMVGFLVSDRPAADR
                     LAAVEEIADWLVRVAIETAAEPSALAPELERLAGHVVPSWKHEGASPALLEGLENVPA
                     VLQHNDLGSWNLVITPGGFTAVDWESARLHGLPLWDLWYFMADVLGHLDGVTGPEQRE
                     RHFLRLFRGELPASRVLFEWTHTAAEASRVPPEAIGRIATLCWLHHGLSPLNRLSDLH
                     SYQPGAIPPYWLKLTRRIAVSWLRDPALGPEWPALPR"
     gene            complement(10130..10900)
                     /locus_tag="VF752_03125"
     CDS             complement(10130..10900)
                     /locus_tag="VF752_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8854566.1"
                     /translation="MRQPRASAIVPFHGKGPLLREAVESALSQSVSDLELIVVDDASP
                     EPAGDALADVADERVRIVRLPRNRGAATARNAALALARAPFVSQLDADDTWEPRYLES
                     VLPRFADPDVGLVYTNARLLGHPQGRQVSYLDSTGHPVRRWEQLAHENALQAPTVTIR
                     AEAARAVGGWATWLRLVEDWHMYLKLFRAGWRFDYVDEPLATYRWPHYGLGGKYARAQ
                     LERFELIMWAGIALRVRRWPRGQIRWRVERELRRGPHD"
     gene            complement(10897..12402)
                     /locus_tag="VF752_03130"
     CDS             complement(10897..12402)
                     /locus_tag="VF752_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8854567.1"
                     /translation="MSQPGTAEFVIVGSGVAGQLVARELLAAGRDVLMLERGGLKPHA
                     QQLADGVHELDIPTAKPNHEPAPGTQDYPWAYVYGVGGTTQHWTGATPRLLPEDFEMH
                     SRFGVGRDWPFGYREVDPWYEEAEATLAVAGGRNPLIPRRKALPLPPHPFSPADKLVA
                     AAVPKFYSFPSARPTRPVDGRPACCGSARCNLCPVDARFSALQLYEREIKGKPGFTLR
                     DRTVVSRLRTSGNRVTGLDCVTADGKRFSLPARNVVLCANGIENAAILMRSDLGGGDV
                     GRYLYDHDEVVLEFGLDRSAGAGIGTTLSTGMTYEYGTGDFRSKRAAFILTPYNPGIE
                     VQELMADELVNGRSGRSLREELQRYFDRTLIIVASGDDLPQRERFVSLSPTKDSLGIP
                     LNRVHYAGPTAYYDAGLQAVREGLEQRFARYGAKLHAHRGSARGGHLIGTCRMGERDG
                     VVDPELRHHKLANLHVAGSSAFPAYSAAHPTLTLSALAIRLGRHLTGQVRQ"
     gene            complement(12399..12800)
                     /locus_tag="VF752_03135"
     CDS             complement(12399..12800)
                     /locus_tag="VF752_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854568.1"
                     /translation="MQGRRRSGLTRRQLVAGGGAAALVLLGGAYGRFAIGDEFEGHLA
                     DVLGVPMDVAKRFTSNAREHFGQGEYDLRAAGFLAATTFPGSLLHPEAVRSRAVGGFV
                     RAMMDGTADNLAYLGLQRQLTGYACNGLIRE"
     gene            12872..13984
                     /locus_tag="VF752_03140"
     CDS             12872..13984
                     /locus_tag="VF752_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854569.1"
                     /translation="MKGARRSRGRRLPAGLLPGLVLLLFALVGLKAAGVGPDLPGPKI
                     FGGDPYAPVNCSPSPRRLKDPSSPPPAPGSWTTGPETPEPGAELVGASVGGLAYLVGG
                     QDAIGKSIATVRTFDPATGRYAQVPDMPFRTDHVIAKGYRGDLYVVAGYHDSEPAAVF
                     MRYSPSERRWQMLAKPPLARGGAGGAVIGHRLYVVGGAPRSSPNARVQPYRMLAYYDF
                     RTGRWGEGTPMPTARHHIGVTALGSSLFVAGGRTPSDLSTNAFESYDTRTRRWSKLPH
                     LPLGVAAMGMVSVSHEVILVGGDDETNWQTGGGWATNASWAYNPATRRWRRLPDLPHA
                     IQAAAVVNVGGRIRTFAGNDCPGLHRTREALSLSAP"
     gene            14036..15163
                     /locus_tag="VF752_03145"
     CDS             14036..15163
                     /locus_tag="VF752_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8854570.1"
                     /translation="MRVSRRRTGRRLPAGFLPGLVLLLFALVGLKAAGVDLPGPRIFE
                     KLGLSEGFTPLQYCSPKPSQIRDPASPKPASGSWRREPNLPVPAAEQVAAVIGGLVYV
                     VGGQDYLGRTTRRVFTFDPATRRYARVPDMPTRADHTVVVAYRGDLYVVAGYLNSDPI
                     AWFLRYSPRTRKWTVMPKPPLARGGAGGGLIGDRLYVAGGAPRSDPNFGVTPYRTLEI
                     YDFKTGRWSRGPDMPTARHHVGGVTVGDKLYIAGGRSGHQQAMNDFEEYDARTNRWTK
                     LAPLPVGVSAMGLVRHGSDVIMSTGGDDDPFRTGEGFVSPQTWAYDTRAGHRGWRRLP
                     DLPRGQHAAAYALADGRLWSFAGIDCPGFHPTGNAYSLTLR"
     gene            15173..15970
                     /locus_tag="VF752_03150"
     CDS             15173..15970
                     /locus_tag="VF752_03150"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX8854571.1"
                     /translation="MIDLHSHVLAGIDDGPQDLDGSLALARAASRDGTTTLAATPHAR
                     ADHPRVQLENVANQCAELNELIPDSWGIEVVPGAEVDIAWAQSASEEQLRLATYLQRG
                     TDILVETPYGPLPSTFEDLLFNLSVRGLRVVLAHPEWNPSFQTDPGRVAAMADRGVLV
                     QITALSLVRPKRRSRARRLAYFLLEEGLAHLIASDSHSGGSWRPPQLRMGVEAASQVV
                     SPARAEWMVTEAPAAVLAGEPLTSAPVSPPSRQGRSFLRRLTGSNGR"
     gene            complement(15957..16535)
                     /locus_tag="VF752_03155"
     CDS             complement(15957..16535)
                     /locus_tag="VF752_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854572.1"
                     /translation="MSEPDTRPPEERISSELGERVRAIIASAENMAAAIRRDAEQDAQ
                     NRRRETEAEAQRYLDEARRRADELVAERVRRISELSDLIVERTESILERLDQAEAVKA
                     QLEQLVRALGATATRVANEARQDETPEAPLPPPLSQEQSEPSPDDLDTARLVALQMAV
                     AGAARDEVETHLARDLGIPDPRPVIEDVFSGR"
     gene            complement(16624..17145)
                     /locus_tag="VF752_03160"
     CDS             complement(16624..17145)
                     /locus_tag="VF752_03160"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8854573.1"
                     /translation="MSDSAMKQIATDQATREASGAHPYGRYLEEFELGAIYKHWPAKT
                     VTESDDHLFCLITMNHHPLHINDVYAKESQQGRNVVVGPLVYSLALGMSVSDVSGKAI
                     ANLATEELSHPAPVFHGDTLFVESEVLEVRPSESKPDRGIVKVRTDVYNQDGVLVATF
                     KRTVLVPRKPDAQ"
     gene            17349..18398
                     /locus_tag="VF752_03165"
     CDS             17349..18398
                     /locus_tag="VF752_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX8854574.1"
                     /translation="MRNPRDFTKPLAVGAPDPVTEIPVKPSRMIHFFDASNEKMAAKV
                     PDMARQADILLGNLEDAVPADRKEAARAGLVKVGKEADLGGTPLWTRVNSLDSPWVLD
                     DLTTLVTEIGDKLEVIMVPKVEGPWDIHYVDQLLAQLEARAGLKRPLLVHAILETAQG
                     VTNVEDIATASPRMQGMSFGPADLAASRRMKTTRVGGGHPGYRVIEDPDPDNPDAPRQ
                     SYQQDLWHYSIGRMVDACAGAGILPFYGPFGDIADVVGCEYQFRAAFLMGCVGAWSLH
                     PKQIEIAKKVFSPEPEEVRWAKKVIEAIPDGRGVHMIDGKMQDDATWKQCKVMVNLAE
                     MLAQKDPELKEAYGL"
     gene            18395..19684
                     /locus_tag="VF752_03170"
     CDS             18395..19684
                     /locus_tag="VF752_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8854575.1"
                     /translation="MRFFEYESRQILEREGIPVAKGGFAKTPEEAERIARDIGEPVVI
                     KSQVLTGGRMKAGGVKFADTPDEAREHAEHILGLEINGHTPRGVLVGHKAPIAKEFYA
                     GVIYDGTRKLPTFVFSDMGGIDIEEVAEQHPGHIGRGHFSTILPHMDFRAKDVVASVG
                     ITGSELNRLTPILSKLARLFVEYDMTLAEINPLAQLEDGSFVAVDAHMDMENEARGRH
                     KQLLVDLGVGEEETREAREPTPFEIEGEKIDSVDHRGVAGNVTEFDGNLGLVIGAGGG
                     SLTLFDAVRNYGGKPANYCEIGGNPSVSKACNLTKLVLSKPGVDKIAVMMSIVSNTRV
                     DIVARGVIKACEELGYDPAEKIAIFRIPGAWEDEGFKILEKYGVEYCDRSVSMHAAAR
                     RAVEKIGGADGKVPVKPAPAGMEDRTSGEVTEVTEAQ"
     gene            19681..20595
                     /locus_tag="VF752_03175"
     CDS             19681..20595
                     /locus_tag="VF752_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX8854576.1"
                     /translation="MSILIDESTTFIVQGITGREAVNLTKECLDYGSKVVGGVTPGRR
                     GREVHGVPVFDSVAQLVQELGYVPDGSVVTVPPAFTKDAVFEAIEAGIKIIVIVTERI
                     PRYDVAQMVELAGLRGARIIGPNCLGIIRPGVAKMGGIGGPAKDAQKAYQPGSIGVMS
                     RSGGMTTEISSTLSAAGLGVSTAISIGGDAIIGSTYAELMPLFEADEQTEAIVIYTEP
                     GGRMEAQLAQWVKQHDSRLPIVAFMAGRFMDKMPGMSFGHAGTIVEGKADTATEKIAR
                     LNEAGIEVAEEIAQIPDLVKQKLGKVAA"
     gene            20595..20837
                     /locus_tag="VF752_03180"
     CDS             20595..20837
                     /locus_tag="VF752_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854577.1"
                     /translation="MSDGMFIQVEVDDSIRGDADLAQQLEDACPVDIYKNAGGHVEIV
                     GRNLDECVLCELCIQAAPAGTVRVKKLYSGEVLQSR"
     gene            complement(20834..21955)
                     /locus_tag="VF752_03185"
     CDS             complement(20834..21955)
                     /locus_tag="VF752_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854578.1"
                     /translation="MLRVLVCGGGVAASEALLRLRKLAGDEVSLTVLAPDDDLVYRPT
                     AVREPFAGGGARRYPFAAIAEAAGADVVKDSLASVDLERSIVRTGQDTELPFDALVVA
                     VGARFSAPFRHARVFTDSSAAMLQGLVQDIEEGYSHSVAFVVPDGPSWPLPIYELALM
                     TRERANSMGADDLEIILAVPEPKPLVAFGDEVSAEVTRLLDEARVTLYSSTRPSVPEA
                     QQLLLQPPGVLLRPERIVALPHMSGPDVHGLPGGTLGGYIPIDNTCAVPGTDGRVFAA
                     GDGADFPIKHGGIGAQEADVAASHIARLAGVDVEPEIFRPHIAGTLLTGGKPLYVEAR
                     VVGNRGFQGQIHETPPWPEDAKIVAVELAPFLETLAPSS"
     gene            22049..22681
                     /locus_tag="VF752_03190"
     CDS             22049..22681
                     /locus_tag="VF752_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854579.1"
                     /translation="MLSNVNADFSARAAKLRRKTLELVDELSQAGLAQDRGASEALIR
                     VVSAANQLGVAEALLDPARGTSAAADAGRPQLSQDAAIVLALAATAVPFAASREDEAE
                     RWVRILRMHGQVGSAMQSLGVPEAPLESPAQDPGSRVEHQAEGGSDVVEAIGDRAADL
                     TRRRAADTVTTVDVFFAVLEAYGPAFDRALYARGATRDDVIARLAAAARV"
     gene            complement(22678..23031)
                     /locus_tag="VF752_03195"
     CDS             complement(22678..23031)
                     /locus_tag="VF752_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854580.1"
                     /translation="MASGDTRTQIEQALGRFIAEVPALGQLKLVMRLELRARGDVPIW
                     RVEVPGPKISKDPAGDARIDVSIDRKEFNELAAEGTLRQWANAYDRGHVRVSGDSAVT
                     KLLGNVIGRQLARAR"
     gene            complement(23047..24255)
                     /locus_tag="VF752_03200"
     CDS             complement(23047..24255)
                     /locus_tag="VF752_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8854581.1"
                     /translation="MAVATPYSEKTDEQRAITEMVRQFVDEQVIPIAEEYDHEDKFPD
                     QVVEQMKELGLFGVTIPEEYGGMGLDLTTYAMIVEELSRGWISVSGIVNTHFIGSYLL
                     MKFGTDEQKEKYLPRMATGEIRAAFSLSEPGLGSDVQAISTRARKDGDQWVINGQKMW
                     VTNGLRAGLVFLLAKSDPDADPPHRGMTCFICEKEPGKDVNEGEFAGLTIPPKIKKMG
                     YKGVESTELVFDGYRCPSVNVLGGEEAGLNKGFGQMMDALEVGRVNVAARGVGIAQRA
                     LELALRYSQERKTFGKPIAEHQAIQFKLADMGTKVEAARLLTLKAARMKDAGERSDLE
                     AGMAKLFASETGKEVVEESFRIHGGYGYSKEYEIERLYRDAPLLLIGEGTSEIQRMVI
                     GRTLLKRNKI"
     gene            24418..25584
                     /locus_tag="VF752_03205"
     CDS             24418..25584
                     /locus_tag="VF752_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854582.1"
                     /translation="MSLFGDGMVPVALAFAVLDRTGSVSGLGLVLAAQALPLALLLLA
                     GGVFADRLPRRSVMLVADLVRCGAQAATAALVLLDVATLWQLALLQALYGAASAFFNP
                     ALTGLTPMVVAGDRLQDANALRGVAAAVGRIGGPAAAGLLVAAASPGFALAADAATFG
                     VSVIALAGLSVPRSPSRRTSLFMSELREGWREFRALPWVVAVVVAVALGGIPWAAFPV
                     LGPVVAKASLGGGFAWGLISAAAGVGALGGGLLALRLRPERPVLAGCLATLSFPLPLG
                     LLALHARIAVIAIAALLAAGSTTFFNALWETALQRHVPAHALSRVAAYDWFGSMALEP
                     VGFAVIGLAAGALGVETTLWLAAVALFMTCLLPLLVPSVWALRASSVAAEEAVG"
     gene            25662..27074
                     /locus_tag="VF752_03210"
     CDS             25662..27074
                     /locus_tag="VF752_03210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8854583.1"
                     /translation="MWRVTPIHTPWGYWLEEAGPVTPEPPLEGDLDADVVIAGGGYTG
                     MWTAWWLTEHAPDVRIVLLEAELAGHGPSGRNGGFCTSLWDDLAPMRERFGNDAALAV
                     ARASSAAVDGVGEWCSRQGVDAWYRAAPHLVVSAAPAQDGAWDEAVAACAEVGEPQEY
                     VALSQDEVRARCDSPVFRGGAMMRSGASVQPARLSLGLRAKLLERGVQICEHSPVRRL
                     EPAPDGSVIAETPGGRVRAGAGVLATGGAMADVKGLRRRLTVASSHIVLTEPVPDVLD
                     EVGWTGGEPIADARAFLHYFRTTNDDRIVFGWAGGRLAYGGQTRGRIEVDPAVTKQAA
                     RDLVRTFPQLRGRRITHAWGGPIDVSPTHLPMFGTLPGRRVHYSVGYTGNGVAPSHLA
                     GQVLASLALDRRDEITRLPLVDPPPVHVPPEPFRWVGGEIIRAATLRKEAAEERGEEP
                     SAVSSFVSRVPKMMGVHIGR"
     gene            27094..27429
                     /locus_tag="VF752_03215"
     CDS             27094..27429
                     /locus_tag="VF752_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8854584.1"
                     /translation="MVDQLPFNELPHGRVAWRFEGHGRGTSVSFFITHGAPGTGPVLH
                     RHPYEEVFIVEDGRATFTVADETVEAEGGHILIVPPQTPHKFVNSGVGELRMVSIHPV
                     EQMVQEDLE"
     gene            27435..27893
                     /locus_tag="VF752_03220"
     CDS             27435..27893
                     /locus_tag="VF752_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854585.1"
                     /translation="MTTGPDHINRHREIRVALPLERAMHLFTPVGERLWAEGWDPEFP
                     AGQDGDGSTPGTVFLTASERGTTYWLVTERGDDYVRYARITPGLWAGIVEVRARKADE
                     DSAVADVGYVLTALSDEGRAELRSFENGYNDYIDGWERDIENAMEAGRIR"
     gene            27987..29141
                     /locus_tag="VF752_03225"
     CDS             27987..29141
                     /locus_tag="VF752_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX8854586.1"
                     /translation="MARSTELPIWARWGSAAAERPWTVGVEDELVLLDPHDWSAANQG
                     DDVQAVLPARLAAQVSNETHACVLELKSSPHETVAQVASELATMRERLGAALYDELGA
                     RCAAAGTHPLAVASDIALSSSERYRKIGSTMRALAHREPTMALHVHVAVPDPSSAVRA
                     LDGLRAELPLLLALSANSPFWRGVDSGFASIRTPIMSMFPRVGIPQRFGSYGRFVDVV
                     ETLIRSGAIPEVGFLWWDARLQPRLGTLEIRVTDAQTRIADVAAIAAVVQCLVRHYAN
                     NPSSNDLEPEVVAENRFLAARDGLRARLIDSSSGLRRPAIDSLAELLEGCRPYAAALG
                     CSSELLAASALAQDPGDVRQRRIAAEKGLAMLPAELAAEFAGSSEPIAAV"
     gene            29199..>29447
                     /locus_tag="VF752_03230"
     CDS             29199..>29447
                     /locus_tag="VF752_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854587.1"
                     /translation="MCRWMAWRGQPVLMDELLFKTQHGLVDQSRHARMGAETTNGDGF
                     GVGWYGTGDGPGVFRSVEPAWSDANLRELAQHIESPLFM"
BASE COUNT         4259 a        10474 c        10376 g         4338 t
ORIGIN      
        1 gtcgccggct cgctcgagca ccctccccca ccacgcggcg gtctcggcag ccatgcgagc
       61 tatcgcggcg tcgtcgacgc cgagcgagtg cagcagctcc cggtcgagcc gctccacgcc
      121 tacgagcggg tcgggacggc cggcccgtcg ctcacgcgcg gctgcgagcc cggcctgcac
      181 cgacatcacc tgctcctcga tccgcgtgat cgagagctgc ccaggatgca tcctgtaacg
      241 gaccaccggg tcagcgagat tggccagcgc ggcgcgctcg gccagccgca gccagaggtc
      301 gaggtcctcg gcaagcctcg cggcgggccg gtagccgcct gcggcgatcg cgagctcgcg
      361 cctgaacgtc gccgcagagt gcacgaacgc cgtgcgctcc tcgaggcgct gcctgatctc
      421 gccgtcgccg agcgggtact ccgccaggcc gagatcgcgc ccgtcgcccg cgatcatcac
      481 gacggcaccg cccaccagac cgagccgttc gtcggactcg aggcgcccaa cttggcgctc
      541 gagccggtcg ggaagcgcga cgtcgtcggc gtcgaggatc gcgacgtacc tcccgcgcgc
      601 cagtccgagg ccggcattca gcgcgtacgg ttgtccgcgg ttctcctggg aatgcactcg
      661 gaagcggtcg tccgcggccg cgaaccggcg cgcgatctcg gcgctcgcgt cgcgcgagcc
      721 gtcgtcgacg accacgcact cccagtcctc gaacgtctgc gacgccaccg agtcgagcgc
      781 ctccgcgagg tgagcctcgc cgtcgaagac cggggtgagt accgtcaccg ccggctcacg
      841 cgcgctcacc gggcggccgc gtagccgtga ctgagcacgc tggcgagccg ccgcgcgagc
      901 gggcgcagac gttcgcctgc gacctgctcg acgagctccg cctcctcggc cggaagcacg
      961 cgcagcggcg gagcgcaaac cgcgaaggcg agcaggccgg ccgcgacgcc cgccggcacc
     1021 tcccacccgc cgtgaacggc gaccaccgcg aggtgcgccg cgagccacgt gaccgcagag
     1081 gcaagcgcca tgcgcagtat cgagccgacc ccccattcga ggccgccgat cgacgtgact
     1141 gcgtagacca cgatcgggac ggtggccgcg acctgcccgc ccgagttggc gagcgcggcg
     1201 ccggtagcgc cgtgcgagcg cacgagcagc cagttgaggc cgagcgtgac gaccgccgcc
     1261 gcgaccccca caacgcctgg tacgagcctg cgaccgaggc ccaccatcag cgcgttcgct
     1321 ccgcccgaga gcgcgacgag cgggaacact gccagcatga tcaggagcgg cgtacgcacg
     1381 cccgcgtagt cgctcccgta cacgagctga accgtcgccg ggccgacggc cagcgccccc
     1441 gcggtgaccg gcaggctcac cagcaagagg aggcggatcg agcggccata tccagagcgg
     1501 agccggtcgg cgtgacccgc gccgtacagc gtggcgaacg cgggagagat cacgctcgac
     1561 acgacacccg gcagcagccc gagagcgccg accgcggcga aagcaatcga gtagaaggcg
     1621 atctgcgtgt cgcccgagta gtggtcgagg aagacgaact cggagcgctt ccagatcacg
     1681 aacgtgagca ccacgccgcc ggccatcagc cccgtgtagc cgagcacgtc gcgcttcagc
     1741 tcgccgatcg gctcgctgtg cgcgtcggac agctcggcga gcacacgccg ggcaagcagc
     1801 gacgtccaca gcaggttggc gcccaccacg gcggcctcga ccgcgaacag cccggtgatg
     1861 ccgccgcccg cggcgagcac cgcgatcgtt gcgggcacgg aaacggcgcc ggtgaccacg
     1921 cccgggcgcg tgctcagccg ccagcgctgc gcgcccgcga gcgcggcgct cggcaccgtc
     1981 gcgaggatga gcaggaagca gacgagcccg gcgaggtacc aggcggcctg cggctccgag
     2041 ccgctcaacc cgatcgccac gagcacaccg gcagccggga cgccgcccag gatcacgagc
     2101 ttccaagccc acgtgaccag cccgcgcacg acctcagggc ggtcgcggcc gagcgtctcg
     2161 cccacgtagc gcatgagcgc gacgttgagg ccgccggtga agatcgacgc cgcggagacc
     2221 tccacgaacg cgatgaagct ctgccgcccc atcccgtccg ggccgaggta acgcgccgcc
     2281 gcaaccgaga tgccgagcgt atacagctgc ggcagcaccc gggcgagcgc gacccacaag
     2341 ccgccgcgcg tcaccgacgc cgccgtggtg gccgtagcgg ccgctcccgc cgcgggccgg
     2401 tcgctgctcg ggggctccgg ccccgaactg gaagctgtcg tgctcatcta ccgttccgcc
     2461 agggcgtgcc gatacacggc gaggatgtcc ctgaagcgcc gctcgcagcc ttatcctagg
     2521 cccagcagag gcgccttccc gatggacggg gcgggctccc gggccgcagg cggattcaca
     2581 tgactccctc cagcgagatg accgagaggg cgcgctcagg cgcgcggaca tgaggcgctt
     2641 cgtggcgccc ccgccaaggg gcgcgccggc tgccgccgga gagccgtcgt tctcggtgct
     2701 gatacccgcc taccaggctg agtcgacggt cgccgagacg gtcgagtccg cactcgcgca
     2761 gacttatccc gcgcaggagg tgatcgtctg cgacgacggc tcgaccgatg gcacggccgc
     2821 ggcgctcgag ccataccgcg atcgcatcgt gtacctgcgc aaggagaacg gcggtggcgc
     2881 gtccgcgctg aaccacgccg ccgcgcgcgc cagcggtgac ttcctcgtga tcctcgactc
     2941 cgacgacgtc tacctgccgg agcggctcga agcgatcgcc gagctcgcgc gcgagcgccc
     3001 ggacgtcggg atgctcatga gcgacgcatg gttcgaggtc gaaggcgagc gcgtcggccg
     3061 cttcgcgcag gagaacggct tcgaggtggc cgaccagcgg cggggcatcc tcgagcgctg
     3121 cttcatcttc gctccggccg tgagccgaga gtccgtggcg gcgatcggcg gctgggacga
     3181 gcagctcgcc atcggctacg actggtcgtg ttggatgcgc ctcatcttca gcggcgtgcg
     3241 cgccggcctg gtggacgagc cgctcatgca ctaccgcctg cgcgaaggca gcctcgcctc
     3301 ctcgcgcgtg gaggcgctgc gcgaccgcgt gcgtgtgctc gaccgcgtgt cggagcagct
     3361 ggagctgagc cagcatgagc gtgagctgtt cacctccgcg cgccacgtcc acgagcggcg
     3421 gctgaagctg gcggacgccg agctcggcct cagcgacggc gcgcccgacg ttcgctcgcg
     3481 cctgcgggcg gttgccactg acgacggctt cgacgctccc acgcgcgcca aggcgcttgc
     3541 tgcgatggcg gcgccgggcg tcgcccggcg gctcgtgcag aggcgcgcca gccaagggcg
     3601 cacacgcctc gtgcggacga gcccgacacc cggcggagca acgcgcgatg actgagggcc
     3661 tgcagcctcc gcgcaccgtg gtgctgcccg agggcgctcc gccgcccggc gcggacgccg
     3721 tcgacatcaa gggcgacggc gcattcgacc tgcccggcac gaccagcggc gccaccgtga
     3781 tgcgcggcgg catctggacc gcggtgagcc gcatgatccc gcaggtctac acgctgatca
     3841 tctcgctggt ggcggcgcgc ttcctcggcc cggacggaat gggccgccag agcttcatcg
     3901 cgttcgtcga gctctccacc atgcagctgt tcacgggcgg gctctcgctc gggatgatga
     3961 ggaacatcgg cgaggcgctc ggcgcaggcc gggcgggcgc ggtgcgagcg atcatcagct
     4021 ggggctggcg gctccaggcg atcggctgcg tggggggcgt gctcgtgctc ggcggcgcgg
     4081 cagcgttcgg tgccgagccc acggcagcct gggtcctagc ggcggtcgca tgcttcatgg
     4141 gcgtgatgca caccgtgccg agcgccgtgc tgatcggcat gcagcgctgg cgcgacgcgt
     4201 cgatcgtcgg cctcgtgacc ggctcgatcg ccgtgccggc aaccatcgcg gtgctcgccg
     4261 ccggcggcgg catcgcgggc atcttcgcgg tcgaggcagc gaccacgacc atgaacctga
     4321 tctggaccac gcgcctcgcg cggcgcagcc tcgacggtct gggcgacggc aaccacgacc
     4381 ccggcagcga ctacaagcgc cagatcgtcc gctacacgct cgccacgacg gtcacgatgg
     4441 tgctcggcct gatcgtctac cgccgctcgg agttcttctt cctcgagcac tactccacga
     4501 acagcgagat cgcgttctac tcgatcgcct tctcggcgat gagcgctgtg gtgctgttgc
     4561 cggaggcttt cggtggagcg ctcgcgccgg cgatcgcgac gctcgtcggg gcgggcgacc
     4621 acgagcgcat ccgctcgggc tacagccgcg cgctccggct cgcgatcatg gcggccctgc
     4681 cgctcaccgc cgcgatcatg acgctggtac cgccgctgat cgagctggtg tacgggcgtg
     4741 cctacgcgga cacgcgcggc atcctgatca tcatggcgat catctttccg atcctgcccc
     4801 tcctcaacat gggcaacgga ttgatgctgg ggctcggcaa gatgcgcgtt cccgtgatcg
     4861 cgagcttcgt ggccgcgttc gtgaacgtgg gcctcgactt cgcgctcatc ccggatcacg
     4921 ccgcgaaggg cgcggccgtc gctaatgcgg cggctcagac cacggtggcg gtgatcctcg
     4981 tcttgtacat ctggcgcctg ctcggcggga tcgtgctgcg cccgcttccg atcgtgctgt
     5041 cggcgctcgc ctctgcagcc gccgcggcag cggccctcgc cgtgctggcc gcagccggca
     5101 ctggagtgcc cggcgtgatc gccgcctcgg tggcgttcgt gctcgcgctg ctcggggccg
     5161 ggcgcatcct gcgcttcctt ccctcggacg acgccgcatg gctcacggag aacatccgcg
     5221 ggcgggggcg caagcccctg gcgcggctct cccggctgct gtcgggatga gaccgttcct
     5281 cgcgcccgcc gccaagggcg agcccgaggc ggccgtgcaa ccctccttct cggtcgcgat
     5341 cgcggcctac caggccgagg ccatcgttgg cgatgcgatc gagtcggcgc tcgcgcagac
     5401 cgtgcccgcg gtcgaggtga tcgtctgcga cgacggctcc acggacggca ccggcgcagc
     5461 gatagagcgc ttcggctcgc gcgtcaccca catccgcaag gagaacggtg gcgaggcctc
     5521 tgccaagaac gcggctgcgc gcgccgcgcg cggggacttc gtggtgtttc tcgatgcgga
     5581 cgacctgttc gagcccgagc gcctggcggc actcggcgag ctggccgcgc accgccccga
     5641 cctcgacatc ctcaccacag acgcgctcct cgagctcgac ggccgtgtcg cgcgccgctg
     5701 ctacgacgcg agctttccgt tcgaggtgga cgaccagcgc cgagccatcc tcgagcgcaa
     5761 cttcctgttc gggctagcgg cggtgaggcg ctcgcgcctc cgcgaggccg ggggcttcga
     5821 cgaggagatt ccgtacgcga ccgactggga cctgtggctg cggctgatca tgtcgggtgc
     5881 gcgggcgggg atggtcgacg aaccgctcgc tcgctaccgc atcagcggcg ggctcagctc
     5941 acaccgggcg cggctgttcg agggtcgggc tcgggtgatc gcgaaggcgc tcgagcgcga
     6001 gcacctcgag ccgcacgagc ggcgcgccgc ggagcacgcg ctcgccgagc agcgcaagcg
     6061 ggcggcgctc gagggcgcgc gcgaggcact cgtggagcgg cgtcccgatg cacggcgacg
     6121 ctcgctcgac gtcgcgctcg gcccgggcca cacgctgccg agccggctca aggcactggt
     6181 gtcggcggcg gcgccggggc ttgcgggtcg tcgtctcgcg gaccggccgc gggagacgac
     6241 cgccggcctg ctgctgccgg attgacccgg cgctcccagc ggcgcggcag cagctcgccg
     6301 gtgaggacct tccagaggaa cccggccacc ccgtcgtgcg ccttgggctc cacgcggcgc
     6361 aggcagaacg cgtcggtgcc ggcaccgttg cgaccgttga gcgtggtgta ggcgccgccg
     6421 aagccggcgc cgatcgctgc cgaccgaacg cgctcgtcgt ggcgcccgtg cggataggcg
     6481 agcagcgcga gcgggctgct gaggtgctcg gccagttcgc gcctcgaggc agcgaggtcg
     6541 ccgcggagct ccgcttcagg gagatcggtg agtgccacgt gcgagcccga gtgcgagccg
     6601 atggccactc cgtcacgctc gagctgcgcg agctcttccc acgaagcgat cggctcgcct
     6661 cccatccacc aggcgccgcg gccgacctca cgcgtcggca cgaacagctg cgccgcttcc
     6721 gccccgcgaa gcggcgtgag gttgtcgctg aagccgtcgt ccgcggtcag caggtagcgg
     6781 cggcggggca gccacgagcg cgcgtcggag tggaagcgct gcagctcctc agcagatagt
     6841 ggccggaagc ccagcaggcg aagcacccgc agctgccgcc ggaacagacg aggcggcatg
     6901 tccatcgcct cctgccctgc cttcatgtcg cccgcgagcc ggtggtagca gagcgccgtg
     6961 taggagctgc gcgcgagccg gcgccattcg tccgcatccg ccgcgtcgcg tacgcccgcc
     7021 cagaacgcgg cgtcgatcgc ggttcgccgc gcgagaccgc ctaacggagc gagcagccgc
     7081 tcggtccgcg ttcggagcac gacgcgccgg agcgcgcgag cgcgccagcc ggcgttgaac
     7141 caggagccca cctgcatctc tgtcacaagt gagggatccg cgcgcagcat cgcgaccgac
     7201 gagcggcccc gctcgcgcgc agcgcgcagg agtgcacgcc ggtcgagcgg caccggctcg
     7261 ccccagctca gctccacccc gcgccggcgc aaccgcacag ccgcggcctc tgggcgagcg
     7321 gcgccctccg ccagcccgag ctcggcgtcc tcgagaagct ctgggggagc cgaaacccgt
     7381 gccacgacgc gtgtgaacgg tccaatgagc ggaaaaaagc tacctggcag cgcttcagca
     7441 cccgagacgc tgtggagcag ggcccgaggt gatcgataag acttgtcgcg tggatcagcc
     7501 aactcgcggc aatagtgccg gaccgcggag acggctgatg atctactccg acgccttgaa
     7561 cgtcggcggc gccgaggtga gcgtcgggca cctgatcgcc gagctcccgg agcgcttcga
     7621 ggtgaccatc gcgggggtgc agccggacgt ggtgcagtcg atcgccgcgc gccggccggg
     7681 cacgaacgtc gaggtgctcg atcgggtgcg ggacaagctc gacgtcgtcg cgatcgcaga
     7741 ccacgtgcgc ctgctgcgcc gtgcgcgtcc cgacatcctt cacgtgaacc tgttcactcc
     7801 ctggagcggc caatacgcgc agctcgcggg cgtagcgacg cccggcgtgc ggaccgtggc
     7861 agtcgagcat ctgccgatgc cggccacgca tcgccgccag cgattcgcaa agcgcgcgct
     7921 gtcgcgagcg gtgtccgcgc acgtagcggt gggcgagcgt tcggcgaggg agatcgagca
     7981 gctcgtcggg ctgccgaccg agagcatcat gacgatctac aacggcgtgc cccagaacgc
     8041 cccgccgcca ctcgagaggc gggcggacgg gccggtggtg ggctcggtcg ggcggctgag
     8101 cgagcagaag ggttacgacc tgctggtccg cgcgctgccg gacctgcccg acgcacagct
     8161 cgtacttgtg ggagacgggc cggagcgcgc ccggctcgag tcgctcgcgc gcgagctcgg
     8221 cgtgagcgag cgcctgcgga tcacgggctg gagcgacaag gtgagctcgc acctcgccta
     8281 cttcgacgtg ttcgcgcttc cgtcgcgcta cgaggccttc ccgctcgcga tcctcgaggc
     8341 gatgttcgcc gcggtgcctg tcgtagcggc cgaggtggga agcgtgcccg aagccgtcca
     8401 gccaggcgtc accggattga cggtcgcccc gggcagcgcc agcgagcttg cgagcgcgat
     8461 tcgcagcctt ctcgccgatc cggagcgggc gcgggagctc ggccgcaacg cgcggcgaat
     8521 ggcgctcgac cgcttcagcg tgcgcgcgat gacggatcga tacatagctc tgtacgaccg
     8581 gttggccggc tgaacgacgc gccgcgacat tggcgccgcg tcacgaggcg ggcttacgat
     8641 ggagagcact tgcggaggtt cgccccctga tgtccgcggc gctcacggat gcgcgctttg
     8701 cgctgccgac ccctgtccgc acagcagccg tcatcggcgg gctgagcggc tgggcagaac
     8761 cgctcgcggc cgcggggatc gaggtgagcg cgtcgctcga cggcgggccg gcgccggacc
     8821 tggtcgtggc tccgggacgc ctggcacgcg ccgccgtgac gagcggtgcc gccggcgtga
     8881 tcgtgcacgg ggccgatgcc tccgcggtcg ccagcgccga gggcatgacc ggccaccgcc
     8941 tgctggcgct gccgcgcctt cacgagcccg tcgcgtttct agcgctcggc aaccccgacc
     9001 tcgcgcgcta cgcgctgcag gagatgctcg tgccgacgcg ccgctggaag gaggtgcgca
     9061 accgcgccct gctgcctgtg ctgtcgcgcg ggctcgtgcc gtcgcggacc caggtcactg
     9121 tggcctccca caggcgcggc gacccgttcc tcgtcgcggc ggcacgccag ctcggcatac
     9181 cggagcaggc gacctggctg ctctcgccgg gccagggcga tgtgctcgcg cgcggcgcct
     9241 ttcacctgtt cccggaggga tccgatcacc cctcgcacgt gctcaagttc agccgcattc
     9301 ccggtcggtc ccagcccttc gaccgcgatg agcacggcct ggcggtggcc gaagccggag
     9361 gtcccaccgt cgcgccgcac gcgccgcggc tgctcggccg cttcgaggag gcgggcatgc
     9421 acgcgtcggt ggagacggcg gcccgcgggc gccagatggt cggtttcctg gtgtccgatc
     9481 gccccgccgc ggaccgactc gccgccgtgg aggagatcgc ggactggctc gtgcgggttg
     9541 cgatcgagac ggcggccgag ccgtcggcgc tggcccccga gctcgagcgg ctggccggcc
     9601 acgtcgtgcc ctcgtggaag cacgagggtg cctcgcccgc gctgctcgag ggcctcgaga
     9661 acgtgcccgc cgtcctccag cacaacgacc tcggcagctg gaacctcgtg atcacgccgg
     9721 gcggcttcac cgccgtcgac tgggagagcg cgcgactgca cggccttccg ctttgggacc
     9781 tctggtactt catggccgac gtgctcgggc acctcgacgg cgtgacgggc ccggagcagc
     9841 gagagcgcca tttccttcgc ctgttccgag gtgagctgcc ggcctcgcgg gtgctgttcg
     9901 agtggactca caccgccgcc gaggcgtcgc gcgtgccgcc cgaggcgatc gggcggatcg
     9961 cgacgctctg ctggctgcac cacggcctct cgccgctcaa ccggctctcg gacctccaca
    10021 gctaccagcc cggcgcgatc ccgccctact ggctgaagct cacccgcagg atcgccgtct
    10081 cctggctgcg cgatcccgcc ctgggccccg agtggcccgc gctgccgcgc tagtcgtgcg
    10141 gcccgcggcg cagctcgcgt tcgacccgcc agcggatctg tccgcgcggc cagcggcgca
    10201 cgcggagcgc gatccccgcc cacatgatca gctcgaagcg ctcgagctgg gcacgcgcgt
    10261 acttgccgcc caagccgtag tgcggccagc gataggtggc gagcggctcg tccacgtaat
    10321 cgaagcgcca gcccgcgcgg aacagcttca ggtacatgtg ccagtcctcc accagccgca
    10381 gccaagtggc ccacccaccg accgcgcgcg ccgcctcggc gcggatcgtg accgtgggag
    10441 cctgcagcgc gttctcgtgc gcgagctgct cccagcgcct gaccggatga ccagtcgagt
    10501 cgaggtacga gacctgcctg ccctgagggt gcccgagcag ccgcgcgttg gtgtagacga
    10561 gtcccacatc gggatcggcg aagcgcggca gcaccgactc gaggtagcgc ggctcccagg
    10621 tgtcgtcggc gtcgagctgc gacacgaacg gtgcgcgcgc gagcgcgagg gcggcgttgc
    10681 gagcggtcgc ggcgccgcgg ttccgcggca ggcgcacgat tcgaacccgc tcgtcggcga
    10741 cgtcggcgag cgcatcaccc gccggctccg gcgaggcgtc gtcgaccacg atcagctcga
    10801 gatcggagac gctttgcgac agcgcactct cgaccgcctc gcgcaggagc ggacccttgc
    10861 cgtggaacgg cacgatcgca gacgcgcgcg gctgcctcac tggcgcacct ggcccgtgag
    10921 gtgacggccg agacggatcg cgagcgcgga gagcgtgagc gtgggatggg ccgcgctgta
    10981 ggccgggaac gccgagctgc cggcaacgtg caggttggcg agcttgtggt ggcgcagctc
    11041 ggggtccacg accccgtcgc gctcgcccat ccggcaagtg ccgatcaggt gcccgcctcg
    11101 cgccgacccg cggtgagcgt ggagcttcgc cccgtatcgc gcaaagcgct gctcgagtcc
    11161 ctcgcgaacg gcctgcagac cggcgtcgta gtacgcggtg gggccggcgt agtgcacgcg
    11221 gttgagcggg atgccgagcg agtccttggt cggcgagagg ctcacgaagc gctcgcgctg
    11281 aggcagatcg tcgcccgagg cgacgatgat gagcgtgcgg tcgaagtagc gctgtagctc
    11341 ctcccgcagc gaccgcccgc tcctcccgtt cacgagctcg tcagccatca gctcctgcac
    11401 ttcgatcccg gggttgtagg gcgtgaggat gaacgcggca cgcttggagc ggaaatcgcc
    11461 ggtgccgtac tcgtacgtca tccccgtcga gagcgtcgtc ccgatcccgg cgccggccga
    11521 gcggtcgagc ccaaactcga gtaccacctc gtcgtggtcg tagaggtaac ggccgacgtc
    11581 tccgccgccg aggtctgagc gcatcaggat cgcggcgttc tcgatcccgt tggcgcacag
    11641 caccacgttg cgcgcgggca gcgagaagcg cttcccgtcg gcggtcacgc agtcgaggcc
    11701 ggtgactcgg ttgccgctcg tgcgcagccg cgagacgacc gtgcggtcgc gcagcgtgaa
    11761 ccccggcttg cccttgatct cacgctcgta gagctggagc gccgagaagc gggcgtcgac
    11821 cgggcagagg ttgcagcgcg ccgagccgca acacgcaggt cgcccgtcca ccggtcgcgt
    11881 cggccgcgcc gacggaaacg aatagaactt cggcaccgcg gctgcgacca gcttgtcggc
    11941 gggcgagaag ggatgaggag gcagcggcag cgctttgcgc ctcgggatca gcgggtttcg
    12001 tccgcccgcc accgcgagcg tcgcctccgc ctcctcgtac caggggtcca cctcgcggta
    12061 gccgaacggc cagtcccggc ccacgccgaa gcggctgtgc atctcgaagt cctcggggag
    12121 caggcgcggc gtcgcgcctg tccagtgctg cgtcgtgccg cccacgccgt acacgtacgc
    12181 ccatgggtag tcctgcgtgc cgggcgccgg ctcgtggttc ggcttcgcgg tcgggatgtc
    12241 gagctcgtgc acaccgtcgg cgagctgctg cgcgtgcggc ttgagccctc cgcgctccag
    12301 catgagcacg tcgcggcccg ccgcgagcag ctcgcgcgcc acgagctgtc cggccacgcc
    12361 ggagcccacg atcacgaact cggccgtccc tggttggctc actcgcgaat gagcccgttg
    12421 caggcgtagc ccgtgagctg gcgctggagg ccgaggtagg cgaggttgtc ggcggtgccg
    12481 tccatcatcg cgcgcacgaa gccaccaacg gcgcggctgc gcacggcttc cggatgaagc
    12541 aggctccccg ggaacgtggt cgcggccagg aagccggcgg cgcgcaggtc gtactcgccc
    12601 tggccgaagt gctcgcgcgc attggacgtg aagcgcttcg ccacgtccat cggcacgccg
    12661 agcacatcgg cgaggtggcc ctcgaactcg tcaccgatcg cgaagcggcc gtaggcacct
    12721 cctagcagga ccagtgcggc tgcgccccct cccgcgacga gctggcggcg ggtgagcccg
    12781 ctgcgacgtc gcccttgcat gagggtccac agtaaccccg aaggcggcgg tttggaccga
    12841 tttcgccgcc ggcggcttta ggatcgcccg cgtgaagggc gcccgccgca gtcggggccg
    12901 ccgtctgccg gcgggcctgc ttccaggcct cgtgttgttg ctgttcgcgc tcgtcggcct
    12961 caaggcggcg ggcgttggac cggacctgcc ggggccgaag atctttggtg gcgacccgta
    13021 cgcgccggtg aactgcagcc caagcccgcg caggctgaag gacccgtcga gcccgccccc
    13081 cgcccccggc tcgtggacga cgggcccgga gacacccgag cccggcgcgg agctggtggg
    13141 cgcctcggtc ggcgggctcg cgtatttggt cggcggccag gacgcgatcg gcaagtcgat
    13201 cgccacggtg cgcactttcg atcccgccac aggccgctac gcacaggttc cggacatgcc
    13261 gttccgcacc gaccacgtga tcgcaaaggg ctaccgcggc gacctgtacg tggtggcggg
    13321 ctaccacgac tccgagccgg cggccgtatt catgcgctac tcgccgagcg agcggcgctg
    13381 gcagatgctc gccaagccgc ctctcgcgcg cggcggcgcg ggtggcgcgg tgatcgggca
    13441 ccgcctatac gtggtgggcg gtgcgccgcg ctcgtcgccc aacgcgcgcg tgcagccgta
    13501 ccggatgctc gcctactacg acttccgaac aggtcgatgg ggcgagggga cgccgatgcc
    13561 cacagcgcgg caccacatag gcgtcacggc cctgggcagc agtttgttcg tcgcgggcgg
    13621 gcgcacgccc agtgacctct ccacgaacgc cttcgagagc tacgacaccc gcacgagacg
    13681 ctggtcgaag ctgccccacc tgccgctcgg cgtagcggcg atggggatgg tgtccgtcag
    13741 ccacgaggtg atcctcgtgg gcggcgacga cgagacgaac tggcagaccg gcggcggctg
    13801 ggccacgaac gcgagctggg cgtacaaccc cgccacgcgc cgctggcgcc ggcttcccga
    13861 cctgccccac gccatccagg cagcggccgt cgtcaacgtc ggaggccgga tccgcacgtt
    13921 cgccggcaat gactgccccg gcctccaccg tacgcgtgag gcactctcgc tgtcagctcc
    13981 ctgaccgcac gttttttccc gcattccgag cgattcgggc ttacgatgga cgccgttgag
    14041 agtcagccgt cgcagaacgg gccgccgcct gccggccggc tttcttccgg ggctagtgtt
    14101 gttgctcttc gcccttgtgg ggctgaaggc ggctggcgtc gacctccccg ggccgaggat
    14161 cttcgagaag ctcggccttt ccgaaggctt cacgcctctc cagtactgct cgccgaagcc
    14221 gagccagatc agggatccag ccagcccgaa gccggcctcc ggctcgtggc ggcgcgagcc
    14281 gaacctcccg gtgcccgcgg ccgagcaggt cgcggctgtc atcggcggtc tcgtctacgt
    14341 cgtcggcggc caggactatc tcggcaggac cactcgccgg gttttcacat tcgatcccgc
    14401 cactcgccgc tacgcacgtg tgcccgacat gcccacgcgc gcggatcaca ccgtcgtcgt
    14461 ggcctaccgc ggtgatctgt acgtggtcgc cggctatctc aactccgatc cgatcgcgtg
    14521 gttcctgcgc tactcgccgc gcacgcgcaa gtggaccgtg atgccgaagc cgccgctcgc
    14581 ccgcggcggg gccggcggcg ggctgatcgg ggatcggctg tacgtcgccg gcggggcccc
    14641 gcgctccgat ccgaacttcg gggtcacgcc gtatcgcaca ctcgagatct acgacttcaa
    14701 gaccggtcgc tggagccgcg ggccggacat gccgacggct aggcaccacg tcggcggcgt
    14761 gacagtcggc gacaagctgt acatcgccgg cggcaggagc ggtcaccagc aggcgatgaa
    14821 cgacttcgag gagtacgacg cccgcacgaa ccgctggacg aagctcgcgc cgcttccggt
    14881 tggagtgtcc gcgatgggcc tcgtgcggca cggctctgac gtgatcatgt ccaccggggg
    14941 cgacgacgac cccttccgta ccggcgaggg cttcgtgtcg ccccagacat gggcctacga
    15001 cacgagggcg ggccatcgcg gctggcgccg gctgcccgat cttccccggg gacagcacgc
    15061 ggcagcgtac gcgctcgccg acgggcggct ctggtcgttc gccgggatcg actgccccgg
    15121 cttccaccca accggaaacg cctattcgct cacgctacgc tgagcccgcg gcgtgatcga
    15181 cctgcactcc cacgtcctcg cgggcatcga cgacgggcca caggacctcg acggcagcct
    15241 ggcgctggcg cgcgcggcct cgcgcgacgg gacgacgacg ctcgccgcca cgccgcacgc
    15301 ccgcgccgac cacccgcgcg tgcagctcga gaacgtagcc aatcaatgcg ccgagctgaa
    15361 cgagctgatc cccgactcat ggggcatcga ggtcgtgccc ggcgcggagg tcgacatcgc
    15421 gtgggcccag tccgcgagcg aggagcagct gcgcctcgcc acctacctcc agcgcggcac
    15481 cgacatcctc gtcgagaccc cgtacgggcc gctccccagc accttcgagg acctgctttt
    15541 caacctcagc gtgcgcgggc tgcgcgtggt actcgcgcat cccgagtgga accccagctt
    15601 ccagaccgac cccggacgcg tcgccgccat ggccgaccgt ggcgtgctcg tgcagatcac
    15661 ggccctgtcg ctcgtgcgcc cgaagcggcg ctcgcgcgcg aggcggcttg cgtacttcct
    15721 gctcgaggag ggactcgcgc acctgatcgc ctccgattcg cactcgggtg gcagctggcg
    15781 tccgccgcag ctgcgcatgg gcgtcgaggc ggcgtcgcag gtggtctcgc ccgcgcgggc
    15841 cgagtggatg gtcacggagg ctccggccgc cgtgcttgcc ggcgagccgc tgacgagtgc
    15901 ccccgtgtcg ccgccgagcc ggcagggccg cagcttctta cggcgactga ccgggtctaa
    15961 cggccgctga acacgtcttc gatcacgggc cgcgggtcgg ggatcccgag gtcgcgggcg
    16021 aggtgcgtct ccacctcgtc gcgcgccgcg ccagcgaccg ccatctgaag tgccaccagc
    16081 cgcgcggtgt cgaggtcgtc gggcgacggc tcgctctgct cctgcgaaag cggcggcggc
    16141 agcggcgcct caggcgtctc gtcctggcgc gcctcgttcg cgacccgggt ggcggtggca
    16201 cccagcgccc ggacgagctg ctcgagctgc gccttcaccg cctcagcctg gtcgaggcgc
    16261 tcgagtatcg attccgtccg ctccacgatc aggtcggaca gctccgagat gcggcggacg
    16321 cgctcggcga caagctcgtc ggcccgcctg cgcgcctcgt cgaggtagcg ctgcgcctcc
    16381 gcctcggtct cgcgccggcg gttctgcgcg tcctgctcgg cgtcgcgccg gatcgcggcc
    16441 gccatgttct ccgcggaggc gatgatcgcg cgcacgcgct cgccgagctc cgaggagatc
    16501 cgttcttcag ggggccgtgt gtcgggttcg ctcacgggtc aagacagccg ggagtcgcgt
    16561 cccctgctgg cgcggctcgg aacttaccac cggacccgga agttcaacgc gagcgtgcga
    16621 tcgctattgc gcgtccggct tgcggggcac gagcactgtt cgcttgaagg tggccacgag
    16681 cacgccgtcc tggttgtaga cgtcggtgcg caccttcacg atgccgcggt cgggcttcga
    16741 ctccgacggc cgtacctcga gcacctcgct ctccacgaag agcgtgtcgc cgtggaagac
    16801 gggcgcaggg tgcgagagct cctcggtcgc gagattggcg atcgccttgc cggacacgtc
    16861 gctcaccgac atgccgagcg cgagcgaata cacgagcggg ccgacgacga cgttccgccc
    16921 ctgctgcgac tccttcgcgt acacatcatt gatgtgcagc gggtggtggt tcatcgtgat
    16981 caggcagaac agatggtcgt ctgactccgt gaccgtcttc gcgggccagt gcttgtaaat
    17041 cgcacccagc tcgaactcct cgaggtaacg gccgtacgga tgggccccgg acgcctcgcg
    17101 cgtggcctgg tccgtggcga tctgcttcat cgcgctatcg ctcatcaagt cccttggtcg
    17161 gtttgctgaa gtgcctcgcg cgccgggcgg ccgggaagcg gcgcagcccg cgcggcgcat
    17221 tggttgactg atcagtcaac ggacatcgaa tcttacggaa cggtagattt ccccgccccc
    17281 ggggcgccgc tccggcgacg ccggccacgc cgcgcgcccc gtcagtagcc ggacaggaga
    17341 cctcaacctt gcgcaatccc cgcgacttca ccaagccgct cgcagtaggc gccccggacc
    17401 cggtcaccga gatcccggtg aagccctcgc gcatgatcca cttcttcgac gcgagcaacg
    17461 agaagatggc cgccaaggtg ccggacatgg cacggcaggc cgacatcctg ctcggaaatc
    17521 tcgaggacgc cgtccccgcc gatcgcaagg aagctgcgcg cgctgggctc gtgaaggtcg
    17581 gcaaggaggc cgacctcggc ggcaccccac tctggacccg cgtgaacagc ctggattccc
    17641 cctgggtact cgacgacctc accacgctcg tcacggagat cggcgacaag ctcgaggtga
    17701 tcatggtccc gaaggtcgag ggcccttggg acatccacta tgtggaccag ctgctcgctc
    17761 agctggaagc acgcgccggg ctgaagcgcc cgctcctcgt gcacgcgatc ctcgagacgg
    17821 cgcagggcgt gacgaacgtg gaggacatcg ccaccgcgag cccgcgcatg caggggatga
    17881 gcttcggccc cgccgacctc gccgcgtcgc gccgcatgaa gaccacgcgc gtgggcggcg
    17941 ggcatccggg ttaccgcgtg atcgaggacc ccgatcccga caaccccgac gcgccgcgcc
    18001 agagctacca gcaagacctc tggcactact cgatcgggcg catggtcgac gcctgcgcgg
    18061 gcgcgggcat cctgcccttc tacggcccgt tcggtgacat cgccgacgtc gtgggctgcg
    18121 agtaccagtt ccgcgccgcg ttcctgatgg gctgcgtcgg cgcctggtcg cttcacccca
    18181 agcagatcga gatcgcgaag aaggtcttct cgcctgagcc cgaggaggtg cgctgggcca
    18241 agaaggtcat cgaggcgatc cccgacggtc gcggcgtgca catgatcgac ggcaagatgc
    18301 aggacgacgc gacctggaag cagtgcaagg tgatggtgaa cctcgccgag atgcttgccc
    18361 agaaggatcc cgagctgaag gaggcctacg gcctgtgagg ttcttcgagt acgagtcgcg
    18421 ccagatcctc gagcgcgagg gcatcccggt cgcgaagggc gggttcgcga agacacccga
    18481 ggaggccgag cggatcgcgc gcgacatcgg cgagccggtc gtgatcaagt cgcaggtact
    18541 cacgggcggg cgcatgaagg cgggaggcgt gaagttcgcg gacaccccgg acgaggcgcg
    18601 cgagcacgcc gagcacatcc tcggcctcga gatcaacggc cacacgccgc gcggcgtcct
    18661 ggtgggccac aaggcgccta tcgccaagga gttctacgcg ggcgtgatct acgacggcac
    18721 gcgcaagctg cccacgttcg tgttcagcga catgggcgga atcgacatcg aggaagtcgc
    18781 cgagcagcat cccggccaca tcggccgtgg acacttctcc acgatcctgc cgcacatgga
    18841 cttccgcgcg aaggacgtcg tggcgtccgt cggtatcacc ggctcggagc tgaaccgcct
    18901 cacgccgatt ctctccaagc tcgcgcgcct gttcgtggag tacgacatga cgctcgccga
    18961 gatcaacccg ctggcgcagc tcgaggacgg cagcttcgtc gccgtcgacg ctcacatgga
    19021 catggagaac gaggcccgcg gccggcacaa gcagctgctc gtggacttgg gcgtgggcga
    19081 agaggagacg cgcgaggcgc gcgagccgac gccgttcgag atcgagggcg agaagatcga
    19141 ttcggtcgac caccgcggcg tcgcgggcaa cgtgaccgag ttcgacggca acctcggtct
    19201 cgtgatcggc gcaggcggcg gctcgctcac gctcttcgac gccgttcgca actacggcgg
    19261 caagcccgcg aactactgcg agatcggcgg caaccccagc gtctcgaagg cctgcaacct
    19321 cacgaagctc gtgctctcga agcctggcgt cgacaagatc gcggtgatga tgtcgatcgt
    19381 ctcgaacaca cgggtcgaca tcgtggcccg cggcgtgatc aaggcctgcg aggagctcgg
    19441 ttacgacccg gcggagaaga tcgcgatctt ccgcatcccg ggcgcctggg aggacgaggg
    19501 cttcaagatc ctcgagaagt acggcgtgga gtactgcgac cgcagcgtgt cgatgcacgc
    19561 ggcggcgcgc cgcgccgtgg agaagatcgg cggcgccgac ggcaaggtgc cggtgaagcc
    19621 cgccccggcc ggaatggagg atcggacctc cggagaggtg acggaggtga ccgaggcgca
    19681 atgagcatcc tcatcgacga gagcacgacc ttcatcgtcc agggcatcac cggccgcgag
    19741 gcggtgaacc tgacgaagga gtgcctggat tacggctcga aggtcgtcgg cggcgtgacg
    19801 ccgggccgcc gcggccgaga ggtgcacggc gtgccggtgt tcgactccgt cgcgcagctc
    19861 gtgcaggagc tcggttacgt gcccgacggc tcggtggtca cggtgccgcc cgcgttcacc
    19921 aaggatgccg tgttcgaggc catcgaggcc ggcatcaaga tcatcgtgat cgtgaccgag
    19981 cgcatcccgc gctacgacgt cgcccagatg gtcgagctcg ccgggttgcg aggcgcgcgc
    20041 atcatcgggc cgaactgcct cggcatcatc cgccctggcg tcgccaagat gggcggcatc
    20101 ggcgggcccg ccaaggacgc tcagaaggcc taccagcctg gctcgatcgg cgtgatgtcg
    20161 cgctccggcg gcatgacgac ggagatctca tccacgctct cggcggccgg tctcggcgtg
    20221 tcgaccgcga tctcgatcgg cggcgacgcg atcatcggct cgacctatgc ggagctgatg
    20281 ccgctcttcg aggccgacga acagaccgag gcgatcgtga tctacaccga gccaggaggc
    20341 cgcatggagg cgcagctcgc ccagtgggtg aagcagcacg actcgcggct gccgatcgtc
    20401 gccttcatgg ccgggcgctt catggacaag atgccgggga tgagcttcgg ccatgccggc
    20461 acgatcgtgg agggcaaggc cgacaccgcc accgagaaga tcgcccgcct gaacgaagcc
    20521 ggcattgagg tggcggagga gatcgcgcag atcccggacc tcgtgaagca gaagctcgga
    20581 aaggtggccg cctgatgtcc gacggcatgt tcatccaggt cgaggtcgac gacagcatcc
    20641 gcggcgacgc ggacctcgcc cagcagcttg aggacgcctg cccggtcgac atctacaaga
    20701 acgcgggcgg ccacgtggag atcgttggtc ggaacctcga cgagtgtgtg ctctgcgagc
    20761 tctgcatcca ggcggcgcct gcggggacgg tgcgggtgaa gaagctctac agcggcgaag
    20821 tgctgcagag ccgctagctg ctcggcgcga gcgtctccag gaacggggcc agctcgacgg
    20881 cgacgatctt cgcgtcctcc ggccacggag gcgtctcgtg gatctgtccc tggaagcccc
    20941 ggttgcccac cacgcgggcc tcgacgtaca gcggctttcc gccggtgagg agggtcccgg
    21001 cgatgtgcgg acggaagatc tcgggctcta cgtcaacgcc tgccagcctt gcgatgtggg
    21061 aggcggccac gtcggcctcc tgagctccga tgcccccgtg cttgatcggg aagtccgcgc
    21121 cgtccccggc cgcgaacacg cgcccgtcgg tgccgggcac ggcgcatgtg ttgtcgatcg
    21181 gtatgtagcc gccaagcgtg ccgccgggca ggccgtgaac gtccgggccc gacatgtgcg
    21241 gcagggccac gatccgctcc ggccgcagga gcactcctgg cggctgtagc aacagctgct
    21301 gcgcctcggg aaccgagggg cgcgtggagg agtagagcgt caccctcgcc tcatcgagca
    21361 ggcgcgtgac ctcagcggac acctcgtcgc cgaaggctac gagcggcttg ggctcgggga
    21421 cggcgagaat gatctcgagg tcatcggcgc ccatcgagtt cgcccgctcg cgcgtcatca
    21481 gcgcgagctc gtagatcgga agcggccacg agggcccgtc cgggacgacg aacgcgacgc
    21541 tgtggctgta gccctcctcg atgtcctgca cgagcccctg gagcatcgcg gctgagctgt
    21601 cggtgaacac gcgcgcatgc cggaacggag cggagaagcg cgctcccact gccacgacca
    21661 gcgcgtcgaa gggaagctcg gtgtcctgcc cagtcctaac gatgctgcgc tcgaggtcga
    21721 ccgaggcgag gctgtctttc accacatccg ccccggcggc ctccgcgatc gccgcgaacg
    21781 ggtagcggcg ggcgccgccg cccgcgaacg gctcgcgcac tgcggtgggt cggtagacca
    21841 ggtcgtcgtc gggcgcgagc acggtgagcg acacctcgtc gccggcgagc ttgcgcagcc
    21901 ggaggagcgc ctcgctcgcg gcaacaccgc cgccacagac cagcacgcgg agcatcgccg
    21961 cagtctacga aggcggcgcg ttcgggtggc cgccgtccac gcgtccatgc gcttcgagcg
    22021 cgcttacaga cgtgcctgct cggcgccgat gctctctaac gtgaacgccg atttcagcgc
    22081 ccgcgccgcc aagctccgcc gcaagacgct ggagctcgtg gatgaactct cccaggccgg
    22141 acttgcccag gaccgcgggg cgtcggaggc gctgatccgc gtggtgtcgg cagccaacca
    22201 gctgggcgtg gccgaggcgc tgctcgatcc cgcgcgcggc accagtgcgg cggccgacgc
    22261 gggccgcccg cagctctccc aggacgccgc gatcgtgctc gcgctcgcgg ccacggcggt
    22321 gcccttcgcc gcctcgcgcg aagacgaggc agagcgctgg gtgcgcatcc tgcgcatgca
    22381 cggccaggtc ggatccgcga tgcagtcgct cggcgttcca gaggcgccgc tcgagagccc
    22441 cgcgcaggat cccggctcgc gcgtcgagca tcaggcggag ggcggctccg acgtggtcga
    22501 ggcaatcggg gaccgcgccg ccgacctaac acgccgccgc gccgccgaca cggtcaccac
    22561 ggtcgacgtc ttcttcgccg tgctcgaggc ttacgggccc gccttcgacc gcgcactcta
    22621 cgcacgcggg gcgacacgcg atgacgtgat cgcgcgactc gcggccgcgg cgcgcgtcta
    22681 gcgcgcgcga gcgagctggc gcccgatcac gttcccgagc agcttcgtca cggcgctgtc
    22741 acccgacacg cgcacgtgcc cgcggtcgta ggcgttcgcc cactggcgga gcgtgccctc
    22801 ggcagccagc tcgttgaact ccttgcggtc gatcgagacg tcgatgcgcg cgtcgcccgc
    22861 cgggtccttc gagatcttcg gtcccggcac ctccacgcgc cagatcggaa cgtcgccgcg
    22921 ggcgcggagc tcgagccgca tcacgagctt gagctgaccg agcgccggca cctcggcgat
    22981 gaagcgcccg agcgcctgct cgatctgggt gcgtgtgtcg ccggaggcca agggtcgtcg
    23041 gccggatcag atcttgttgc gcttcagcag ggtgcgcccg atcaccatgc gctggatctc
    23101 ggaggtgccc tcgccgatca agagcagcgg agcgtcacgg tagagccgct cgatctcgta
    23161 ctccttcgag tagccgtagc cgccgtggat gcggaacgac tcctcgacga cctccttacc
    23221 ggtctccgag gcgaacagct tcgccatgcc ggcctcgagg tcggagcgct cgcccgcgtc
    23281 cttcatgcgg gcggccttga gcgtgagcag ccgcgccgcc tccaccttcg tgcccatgtc
    23341 ggcgagcttg aactggatcg cctggtgctc ggcgatcggc ttgccgaagg tcttgcgctc
    23401 ctgcgagtag cgcagggcga gctcgagcgc gcgctgtgcg atccccaccc cgcgcgccgc
    23461 gacgttcacg cggccgacct cgagcgcgtc catcatctgg ccgaagccct tgttgaggcc
    23521 ggcctcctcg ccgccgagga cgtttaccga cggacagcgg tagccgtcga acacgagctc
    23581 ggtcgactcg acgcccttgt agcccatctt cttgatcttg ggcgggatgg tgagaccggc
    23641 gaactcgccc tcgttgacgt ccttgccggg ctccttctcg cagatgaagc acgtcatgcc
    23701 gcggtgaggc gggtcggcgt ccgggtccga cttcgccagc aggaacacga gccccgcgcg
    23761 caggccgttc gtgacccaca tcttctggcc gttgatcacc cactgatcgc cgtcctttct
    23821 cgcgcgagtg gagatcgcct gcacgtccga tccgaggccc ggttcggaca gtgagaacgc
    23881 cgcgcggatc tcacccgtgg ccatgcgcgg caagtacttc tccttctgct cgtcggtgcc
    23941 gaacttcatg agcagatacg agccgatgaa gtgcgtgttc acgattccgg agaccgagat
    24001 ccagccgcgc gagagctcct cgacgatcat cgcgtaggtc gtcaggtcga gccccatccc
    24061 gccgtattcc tcggggatcg tcactccgaa gagcccgagc tccttcatct gctcgacaac
    24121 ctggtcgggg aacttgtcct cgtggtcgta ctcctcggcg atcgggatca cctgctcgtc
    24181 gacgaactgc cgcaccatct cggtgatcgc gcgctgctcg tcggtcttct cggaataggg
    24241 cgtggcgact gccaattcgg tctcctgcgt ggctcgttga ctcgcgagtc agtttatcgg
    24301 cgcgaagccg tgccggagcc gcccggaccc gcgaagttcc cgggtcccgg catcattccg
    24361 cgccgtgctc ggggcgctcc acgaccgaaa cttccgccgc ttcttcgccg gccaagcgtg
    24421 tcgctgttcg gcgacggcat ggtgccggtc gccctggcgt tcgccgtgct cgatcgcacc
    24481 ggctcggtgt ccgggctcgg gctcgtactc gccgcgcagg cgctaccgct cgcgttactg
    24541 ctcctcgcgg gcggagtatt cgcggaccgg ttgcctcgcc ggtcggtgat gctcgtcgcc
    24601 gatctcgtcc gctgcggcgc gcaggcggcg accgctgctc tggtgctgct cgacgtcgcc
    24661 accctatggc agctcgcgct gctgcaggcg ctgtacggcg ccgcgagcgc cttcttcaat
    24721 ccggcgctca ccggcctcac gccgatggtg gtcgcgggcg atcgccttca ggacgccaac
    24781 gctcttcgcg gagttgccgc cgccgtcggg cgaatcggcg gtccggcggc tgccgggcta
    24841 ctcgtggccg ctgcgtcgcc gggcttcgcg ctcgccgcgg acgcagcgac cttcggcgtg
    24901 agcgtgatcg cgctcgccgg gctctcggtt ccgcgttccc cttcgcggag gacgtcgctg
    24961 ttcatgagcg agctgcgcga gggctggcgg gagttccgcg cgcttccctg ggtcgtcgcg
    25021 gtcgtcgtcg cggttgcact gggcggcatc ccttgggcag cgtttcccgt gctcggcccc
    25081 gtggtggcga aggcgtcgct cggcggaggt ttcgcgtggg gtctcataag tgcggccgcc
    25141 ggggtcggcg cgctcggcgg cggcttactc gcgttgcgtc tacggccgga gcggcccgtg
    25201 ctcgctggtt gcctcgcgac gctctctttc ccgctgccgc tgggcctgct cgcccttcac
    25261 gcacggatcg ccgtcatcgc gatcgccgcc ctgcttgcgg ccggatccac cacgttcttc
    25321 aacgccctct gggagaccgc gctgcagcgg cacgtccccg cccacgcgct ctcgcgagtc
    25381 gcggcatacg actggttcgg ttcgatggcg ctagagccag tgggattcgc ggtgataggc
    25441 ctggcggcgg gcgccttggg ggttgaaacg acgctgtggc tcgccgcggt tgcgctcttc
    25501 atgacctgcc tgctcccgct tctcgtgccg agcgtgtggg cgctgcgagc gagttcggtg
    25561 gccgccgagg aggcggtcgg ctgagtcggc tggggcggca agatcatgac cggatcgtct
    25621 gccgtggcga ccattgcgga tggcggccga ggttcgacag gatgtggcgg gtgacgccga
    25681 tacatacccc ctggggctac tggctcgagg aggccgggcc ggtcacgccc gagccgccgc
    25741 tcgagggcga tctcgacgca gacgtggtga tcgccggcgg cggctacacc ggaatgtgga
    25801 ccgcctggtg gctgaccgag cacgcgccgg acgtgcgcat cgtgctgctc gaggccgagc
    25861 tcgcgggcca cgggccgtcc ggccgcaacg gtggcttctg cacctcgctg tgggacgacc
    25921 tcgcgcctat gcgcgagcgc ttcggcaacg acgcggcgct cgccgtggcg cgtgcctcgt
    25981 cagcggccgt ggacggcgtc ggcgaatggt gctcgcgaca gggcgtcgat gcgtggtacc
    26041 gagcggcacc gcatctggtc gtgtcggccg cgcctgcgca ggacggcgcc tgggacgagg
    26101 cggtcgcggc ctgtgcggag gtcggcgagc cgcaggagta cgttgcgctg tcccaggacg
    26161 aggtgcgcgc ccgctgcgac tcaccggtct tccgcggcgg agccatgatg cggtcgggcg
    26221 cgagcgtgca gcccgccagg ctgagcctcg gactgcgcgc gaagctgctc gagcgcggcg
    26281 tgcagatctg tgagcactcg ccggtacgcc ggctcgagcc tgcacccgac ggcagcgtga
    26341 tcgcggagac tcccggcggg cgcgtccggg ccggcgcggg cgtgctcgcg acaggcggcg
    26401 cgatggccga cgtgaagggt ttgcggcggc gcctgaccgt tgcgtcctcg cacatcgtgc
    26461 tcaccgaacc ggtgcccgac gtgctcgacg aggtcggctg gaccggaggc gagccgatcg
    26521 ccgacgcgcg cgcgttcctc cactacttcc gcacgaccaa tgacgatcga atcgtgtttg
    26581 gctgggcggg tgggcggctc gcctacggcg gccagacgcg cggtcgcatc gaggtcgacc
    26641 ccgccgtgac gaagcaggcc gcgcgcgatc tggtccgcac cttcccgcag ctacgcggcc
    26701 gccgcatcac gcacgcctgg ggcgggccga tcgacgtctc ccccacccac ctgccgatgt
    26761 tcggcacgct ccccggccgt cgtgttcact actcggtcgg ctacaccggc aacggtgtcg
    26821 cgccgtcgca cctcgcgggc caggtactcg cctcgctcgc gctcgaccgc cgcgacgaga
    26881 tcacgcgcct accgctcgtc gacccgccgc cggtgcacgt gccgcccgag ccgttccgct
    26941 gggtcggcgg cgagatcatc cgcgcggcga ccctgcgcaa ggaggcggcc gaggagcgcg
    27001 gcgaggaacc gtcggcagtg tcgtccttcg tctcgcgggt gccgaagatg atgggcgtgc
    27061 acatcggccg gtagctaggg atagggtcgg cacatggtcg atcaactgcc gttcaacgag
    27121 ctgccgcacg gccgcgtggc gtggcgcttc gagggccacg ggcgcgggac gtcggtgtcc
    27181 ttcttcatca cccatggagc gcccggcacc ggcccggtgc ttcaccgcca tccctacgaa
    27241 gaggtcttca tcgtggagga cggccgtgcc acgttcacgg tcgcagacga gaccgtcgag
    27301 gcggagggcg gccacatcct gatcgtgccg ccgcagacgc cgcacaagtt cgtcaattcg
    27361 ggcgtcggcg agctgcgaat ggtcagcatc caccccgtcg agcagatggt gcaggaggac
    27421 ctggagtagg ccggatgacc accggccccg accacatcaa tcgccaccgc gagatccggg
    27481 tcgcgctgcc actcgagcgc gccatgcacc tgttcacgcc ggtcggcgag cggctgtggg
    27541 ccgaaggctg ggatcccgag tttcctgccg gccaggacgg cgacggctcc acgcccggca
    27601 ccgtgttcct caccgccagc gagcggggca ccacgtactg gctcgtgacc gagcgcgggg
    27661 acgactacgt ccgctacgcg cggatcacgc ccggcctttg ggccggcatt gtggaggtaa
    27721 gggcccgcaa ggcggacgaa gacagcgcag tcgcggatgt cggctacgtg ctcaccgcgc
    27781 tgagcgacga aggacgcgcg gagctgcgca gcttcgagaa cggctacaac gactacatcg
    27841 acggctggga gcgcgacatc gaaaacgcga tggaggccgg ccggatcagg tgacgtttcg
    27901 cggcttgcgc gcctttaaca accgctgatc gcgcttcatc tccatcgggt gaggcgcgtg
    27961 cccactgcac gggcgatcct ccggccatgg cgcgatcgac cgagctgccg atatgggcgc
    28021 ggtgggggag cgcggccgcg gagcggccgt ggacggtagg tgtcgaagac gagctggtcc
    28081 tcctcgaccc gcatgactgg tccgcggcca accaaggcga cgacgtgcag gcggtgctgc
    28141 ccgcccggct tgccgcgcag gtgtcgaacg agacccacgc gtgcgttctc gagctcaaga
    28201 gctccccgca cgagaccgtg gcccaggttg cgagcgagct ggccacgatg cgcgagcggt
    28261 tgggcgccgc gctttacgac gagctaggcg cgcgctgcgc ggcggcgggc acgcacccgc
    28321 tcgcggtcgc gtccgacata gcgctgtcgt cgagcgagcg ctaccggaag atcggctcga
    28381 cgatgcgggc gctcgcccat cgcgagccga cgatggcgct tcacgtgcac gtcgccgtcc
    28441 ccgacccttc gtccgcggtg cgcgccctcg atggcctgcg tgccgaactg ccgctgcttc
    28501 tcgcgctgtc ggccaactcg cctttctggc gcggagtcga ttcgggcttc gcgtccatcc
    28561 ggacgccgat catgtcgatg ttccctcgcg tcggcatccc gcaacgcttc gggagctatg
    28621 gacgcttcgt ggacgtggtg gaaaccctga ttcgctcggg cgccattccc gaggtcgggt
    28681 tcctgtggtg ggacgcccgt ctgcagccgc gactcggcac gctcgagatc cgggtcacag
    28741 acgctcagac gcgaatcgcc gacgtcgccg cgatcgccgc ggtcgtgcag tgtctcgtac
    28801 gccattacgc gaacaacccg agttcgaacg acctcgagcc cgaggtggtc gcggagaacc
    28861 ggttcctggc cgctcgcgac ggcctgcgcg cgcggctcat cgacagctcg tccggactgc
    28921 gacggcccgc catcgactcg ctcgccgagc ttctggaggg gtgccggcca tacgcggcgg
    28981 cgctcggctg cagctccgag ctcctagccg cgtctgcgct cgcgcaggat cccggcgacg
    29041 tgcgtcagcg ccgcatcgcg gcggagaagg ggctcgccat gctgccggct gagctcgctg
    29101 ccgagttcgc cggatcgagc gaaccgatcg cggcggtctg acgcccttca tccgcatcgg
    29161 atgatgcatc tggccggcgc ctcgatcacg ctcgccgcat gtgccgctgg atggcgtggc
    29221 gcgggcagcc ggtgttgatg gacgagctgc tcttcaagac tcagcacgga ctagtcgacc
    29281 agagcaggca cgcgcgcatg ggcgccgaga cgaccaacgg cgacggattc ggcgtcggct
    29341 ggtacggcac aggcgacggg ccaggcgtct ttcgcagcgt cgaaccggct tggagcgacg
    29401 caaatcttcg ggagctcgcg cagcacatcg agtcgccgct cttcatg
//
LOCUS       DASVXS010000058        10168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1068148, whole genome shotgun sequence.
ACCESSION   DASVXS010000058 DASVXS010000000
VERSION     DASVXS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068148"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..931)
                     /locus_tag="VF752_03235"
     CDS             complement(161..931)
                     /locus_tag="VF752_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8854588.1"
                     /translation="MDEVFRALADPTRRSLLDELFKEDGQTLTALEERLPMTRFGVMK
                     HLNVLEEAGLVVTRRRGREKLHFLNPVPIRLVHDRWVSKYAEPWAAGLSGLKQRLEDR
                     TMEKVFEIYIKTTPERLWEAITDSEMRQKYSFGVGVESDWTPGSSYTAVHPGAGITIS
                     EGENLEVDPPRRLVQSFNALWGDDVKSEGTSRVTWEIEPVGDSCRLTVVHDQLREGAN
                     DQLYGGWPMILSGLKTLLETGETLTTPGSLRYSGASAA"
     gene            1044..1835
                     /locus_tag="VF752_03240"
     CDS             1044..1835
                     /locus_tag="VF752_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8854589.1"
                     /translation="MTSVRITHIGGPTALIEVGGWRLLTDPTFDPPGRRYSFGWGSMS
                     RKVAGPAMQPADVGPIDAVLLSHDQHGDNLDVAGRALLPNAGAVVTTVAGAKRLGGAT
                     RGLEPWSSTTLEKPGLPAIEVTATPCRHGPPLSRPLAGDVVGFALRWDGQEHGVLWIS
                     GDTVLYDGVREVGTRLDVDIALLHLGGVRFPVSGPFRYTMTARDAVELCGLVRPRTVI
                     PVHYEGWGHFKESRESIEREFAGAPEDVRRSVRWLSIGLATDVPL"
     gene            complement(1842..3215)
                     /locus_tag="VF752_03245"
     CDS             complement(1842..3215)
                     /locus_tag="VF752_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854590.1"
                     /translation="MRFLVILAGAIATLAIAVPASASVPFKFERLPGVAAPGTPAKYN
                     KVGVLRTGARNAPNILILNPGTSASAAYFEPLAKTIVQKAKDWQVWAVERRENLLEDQ
                     SVFDRAKAGKATPQQVYDYYLGWIGNPAIKTHVRLIPDSEVAFGRQWGMRVEIEDLHR
                     VVLAAKKRARHIVMGGHSLGGSITAAYATWNFHGKPGAADLDGLVFIDGGSSPTPVSP
                     ADATRSLQDLQASTPWLAFGGLPTPFAGLFNTSGALSVIVAPNMPSLGQASPLLPASL
                     KPPVRVTNLAQYGYALDTATSPAPLIAAQAHLGHLAPGGAPRGWVRAGELTPIRRYAE
                     MFAGWGLKGLDGTAWYHPRRLTIDAGAVADGNPNPAQKVLGVRATHGRDLPRRLRIYA
                     FGAALGGQRVLGAASSLAKQSRIPKRQLTLVDRHTPYAHNDPNSASPKNDFVKFLLPF
                     LGKVGRG"
     gene            3268..3999
                     /locus_tag="VF752_03250"
     CDS             3268..3999
                     /locus_tag="VF752_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854591.1"
                     /translation="MFDHVTIRAADREASERLYATVLAALGIEQSGSIEMLAEWDDFS
                     LAQASGERPITRGLHIGFTAPSREHVDEFWEVGMAAGLRDDGEPGPRPEYRYDYYGAF
                     LLDPDGNSIEAVHHGGVRQDGNIDHLWIRVADVAASRRFYDVISPHAGYELGHEHDGR
                     AMFRGPSGSFTVLRGEPTENLHMAFPADTNDVVEAFHTAATGAGYLDNGGPGERPVYH
                     PGYYAAFVLDPDGNNIEVVNHNRAR"
     gene            4044..4631
                     /locus_tag="VF752_03255"
     CDS             4044..4631
                     /locus_tag="VF752_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8854592.1"
                     /translation="MAKVLILTGDAAESLEVMYPYQRLLEEGYEVDIAAPSKKKLQFV
                     VHDFVPEFDTYTEKLGYTWDADLAFADVDPSQYVAVVVPGGRAPEYIRNDEDFKRIVN
                     QFFDDTKPVAQLCHAPLALAAAGVLRGRRTAAYPALAPDVEAAGAEFVDGDAVVDGVM
                     VSARAWPDHPSWMREFIRVLRERAPAGAPQAAGVA"
     gene            4733..5413
                     /locus_tag="VF752_03260"
     CDS             4733..5413
                     /locus_tag="VF752_03260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8854593.1"
                     /translation="MGSSLVALLSVIAGLAGHHDSMPRHTGYVVAHVRAGRSLELRAS
                     PGGRSLLRVGDRTEFGTRKTLGVTARRHGWLAVTDPRLANGRRGWLRAGDPRVELTQT
                     EVALHADISRRKLTLERDGRTVEEARVAVGAPSSPTPAGRFAITDKLRGPDYSASYGC
                     CILAMSGSQPRTPAGWRGGNRLAIHGTSSSETIGRASSAGCLRTDDRSLRTMMRKVPV
                     GAPVFIHR"
     gene            5488..6045
                     /locus_tag="VF752_03265"
     CDS             5488..6045
                     /locus_tag="VF752_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854594.1"
                     /translation="MWISKRSLVIGLLAAMTALGMMLAVGAFGAAGGGKAAKHSRPGS
                     QKHGKRHGAPLIKESLAPSQPTDPAFHGVSPGAAPWVLKRGEVRLKSDGKLTLRVKGL
                     VIPIAPGNGTPGPVNTISASLYCGADTNTTATDTTDQVPISRKGDARIKDKTFTVPAT
                     CLAPVILVHPNGDGTHYIAVDGWRL"
     gene            complement(6042..6902)
                     /locus_tag="VF752_03270"
     CDS             complement(6042..6902)
                     /locus_tag="VF752_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8854595.1"
                     /translation="MSTAFVLAGGASLGAIEAGMAEALYERGIRADYMVGTSAGGLNA
                     GFLATNPQEVASAHKLQDTWRGVTRRQVFPFSPATVVLGLLGRRNHLVSPRGLRRLIE
                     RQLDIERLEATDTPLGIVVTDLLCGQEKLVENGSAEDALAATAAIPGIYPPVEIDGRL
                     CVDGGVADNTPISCAVERGCDEIYVLPTGMSTEIDEPPAGALAIGVHAATILLHERLR
                     REVEEYRDRVRLVVLPPPWPLDVLPTDFSKADSLISRGLENARKALAEPEPEGQPTDE
                     AMRRMTAETH"
     gene            6958..7809
                     /locus_tag="VF752_03275"
     CDS             6958..7809
                     /locus_tag="VF752_03275"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8854596.1"
                     /translation="MANQSRAVLITGCSSGIGHATADRLARAGKRVYATARRPDAIAD
                     LAESGCELLALDVTDEESTRSAVEHVEEREGAVGVLVNNAGYSQSGAIESVPMEAVRR
                     QFETNVFGLVRLTQLVLPAMRAQRWGRIVNLSSIGGRVVFPGGGFYHATKYAVEAISD
                     ALRFEVEGFGIGVTVIEPGLIRTRFGEVAASSVRDETAGRGAYDDFNEAVATTTAGAY
                     ANGLLAKFSGTPDDVAKAIEKAIDAERAPTRVPVTASAHLVIPQRKITPDRVWDRMMR
                     VAFPRPK"
     gene            complement(7810..8532)
                     /locus_tag="VF752_03280"
     CDS             complement(7810..8532)
                     /locus_tag="VF752_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8854597.1"
                     /translation="MSDFTDDHDRFTTAYLGALGIDEGWRCLDVGAGDGTIARWMSHQ
                     VAPNGRVVALDVNDERLRKHLQGTRVEVRKHDAAAPEPLPDGPYELVHSRVLLGSLPS
                     KFEVLRRMIDAVAPDGILALGELDFTVHGPVEPDEAWDRVWGTFMRGMAQSGWDPAYG
                     KQLPEALERNGLLEVQAETVSRYVPGGSLPCIITLKAIERARPRLTEVDRFEIDAAQD
                     LLSDPRRKFTAPDFVMASGRAV"
     gene            complement(8582..>10168)
                     /locus_tag="VF752_03285"
     CDS             complement(8582..>10168)
                     /locus_tag="VF752_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8854598.1"
                     /translation="VSGAESQSGRPLAVFGPQTGYFAPQILMDQDVHAPTIDARGAGF
                     PGTNLYVELGRGRDYAWSATSAGQDIIDTFALPLCNPDGSKPTLDSDHYRFHGSCRQI
                     EKLDRTNSWQPNAADQTPAGSETLHAQRTALGIVAGKGTVRGKPVVYSKLRSTYMHEV
                     DSALGFSDFNNPDKMRTPQDFQNAAYKIGYTFNWLYVDDKHDAYFNSGNNPVRPKGID
                     TNFPTMGLKRFEWRNFNPADLTADYTPQSAHPQVVDQSYITSWNNTQARGYRAADDNF
                     SFQSVYRSKPLDDGIKTRIRGAKKINLPGLIDAMEEAGTVDLRGNAVLPWMLRVLGPQ
                     KDPHLQNAINILTQWAKGGAHRRDKNRDGVYDQAEAVRIMDAWWPLWIHAQFEPVLGK
                     ELFDAIAKVNEIDNAPNNHGDHLGSAYQAGWYGYALKDLRTISGKKVKGPYSRVYCGR
                     GKVTRCRALLASTLRQAVNTPASKIYSGDEVCKAEKKDGNQWCYDAVRQRPLGGVTQP
                     LIHWINRPTFQQAVEIESHR"
BASE COUNT         1485 a         3473 c         3576 g         1634 t
ORIGIN      
        1 gacggaaagt cgaggcgaac catcacctcg ctcccccctt gctggtcgac cgccacgatc
       61 tcctgtgctc cccagtccga gacccacaga cggccatcgg ggcccgggcg cggcgactcg
      121 ccgaagatga gccccgacag cagcgtccga gtctcggcgg tcacgcggcc gaggcgcccg
      181 agtagcggag cgagcccggc gtggtgagcg tctcgcccgt ctcgagcagg gtcttgaggc
      241 cggagaggat catcggccag ccgccgtaga gctggtcgtt cgcgccctcg cggagctggt
      301 cgtgcacgac ggtcagccgg cacgagtcgc ccacgggctc gatctcccat gtcacgcgcg
      361 aggtgccctc gctcttcacg tcgtcgcccc agagcgcgtt gaagctctgc acgagccggc
      421 gcggcggatc cacctcgagg ttctcgccct cgctgatggt gatgccggcg ccggggtgga
      481 ccgccgtgta gctcgatccg ggcgtccagt cggactcgac gcccacgccg aagctgtact
      541 tctggcgcat ctcgctgtcg gtgatcgcct cccagagccg ctccggggtg gtcttgatgt
      601 agatctcgaa gaccttctcc atcgttctgt cctccagtcg ttgcttgagg ccgctgaggc
      661 ctgcggccca cggctcggcg tacttgctca cccagcggtc gtggacgagc cggatcggga
      721 cggggttcag gaagtgcagc ttctcccggc cccggcgacg ggtcacgacg agacccgcct
      781 cctcgagcac gttcaggtgc ttcatcacgc cgaagcgcgt catcgggagc cgctcctcga
      841 gtgcggtgag cgtctgaccg tcctccttga acagctcgtc gaggaggctg cgccgggtgg
      901 gatccgcgag agccctgaac acttcgtcca tggccgagag aatacgtgac catatggtca
      961 catgtcaacg cccgagtgat cgttcacaca ctcgaggttg ctcttcaagc tctccggctg
     1021 gttagctagc cgcccacgaa ccgatgacga gcgttcgcat cacccacatt ggcgggccca
     1081 ccgccctcat cgaagtcggc ggctggcggc tgctcaccga tccgacgttc gatccgcccg
     1141 gccggcggta ctcgttcgga tggggctcga tgtcgcgcaa ggtcgcggga cccgcgatgc
     1201 agcccgccga cgtcggcccg atcgacgcgg tgctactgag ccacgatcag cacggcgaca
     1261 acctcgacgt tgccggccgc gcgctgcttc cgaacgcggg cgccgtggtg acgacggtgg
     1321 cgggagccaa gcggcttggc ggggcgacac gcgggctcga gccctggagc agcacgaccc
     1381 tcgagaagcc cggcctgccc gcgatcgagg tgactgcgac tccgtgccgc cacggcccgc
     1441 cgctgagccg tccgctcgcg ggcgacgtgg tgggcttcgc gctccgctgg gacggacagg
     1501 agcacggcgt cctgtggatc tcaggtgaca cggtgctcta cgacggagtg cgggaggtcg
     1561 gcacgcgcct ggacgtggac atcgcgctgc tccatctcgg aggcgtgcgc tttccggtct
     1621 cgggcccgtt ccgctacacg atgacggcga gggacgcggt ggagctgtgc ggtctggttc
     1681 ggccgcggac ggtcatcccc gtccactacg agggctgggg gcacttcaaa gagagccggg
     1741 agtcgatcga gcgcgagttc gcaggcgccc cggaagacgt gcggcgaagc gttcgctggc
     1801 tgtcgatcgg ccttgcgacc gatgtgcctc tctgagccgg gttagccgcg cccgaccttc
     1861 ccgaggaacg gcagcaggaa cttcacgaag tcgttcttgg gcgatgccga gttcgggtcg
     1921 ttgtgcgcgt acggcgtgtg ccggtcgacg agcgtcagct ggcgcttggg tatgcgcgac
     1981 tgcttggcca gcgagctcgc ggcgccgagc acgcgctggc cgccgagcgc ggcgccgaag
     2041 gcgtagatgc gcagccgccg cggtaggtcg cggccgtggg tggcgcgcac accgagcacc
     2101 ttctgcgcgg ggttcgggtt gccgtccgcg acggcgcccg cgtcgatcgt gagccggcgc
     2161 gggtgatacc acgcggtccc gtcgaggccc ttgaggcccc agccggcgaa catctcggcg
     2221 tagcggcgga tcggcgtgag ctcgccggcc cgtacccagc cgcgcggagc gccgccgggc
     2281 gcgaggtgtc cgaggtgcgc ctgggcggca atcagcgggg ccggggaggt ggcggtgtcc
     2341 agcgcgtagc cgtactgcgc gaggttcgtg acccggaccg gcggcttgag gctcgccggc
     2401 agcagcggtg aagcctggcc aagcgacggc atgttgggag cgacgatcac gctcagcgct
     2461 ccgctcgtgt tgaacaggcc cgcgaatggc gtggggagcc cgccgaaggc gagccacggc
     2521 gtcgacgcct gcaggtcctg gagcgacctg gtcgcatcgg cggggctgac tggcgtcggg
     2581 ctgctgccgc cgtcgatgaa cacgagcccg tcgaggtccg ctgcgcccgg cttgccgtgg
     2641 aagttccagg tggcgtaggc ggccgtgatc gaaccgccga gcgagtgacc gcccatcacg
     2701 atgtgccttg cgcgcttctt ggcagccagc acgacccggt ggaggtcctc gatctccacg
     2761 cgcatgcccc actgacgccc gaaggcgacc tcggagtctg ggatcaggcg aacgtgcgtc
     2821 ttgatcgccg ggttgccgat ccagccgagg tagtagtcgt agacctgctg cggcgttgcc
     2881 ttgcccgcct tggccctgtc gaacaccgac tggtcctcga gcaggttctc ccgccgctcg
     2941 actgcccaca cctgccagtc cttcgccttt tgcacgatcg tcttggcgag tggctcgaag
     3001 taggcggcgc tcgccgacgt gcccggattg aggatgagga tgttcggcgc gttccgcgcg
     3061 cccgtcctca ggactcccac cttgttgtac ttggccggcg ttccaggtgc ggcgacgccc
     3121 ggcaggcgct cgaacttgaa cgggacggag gccgacgcgg gtacggcgat cgcgagcgtc
     3181 gcgatcgcgc cggccaggat gacgaggaag cgcacggtcg gctcttctac cagaccgccg
     3241 ccaccgcgga caagtaccct gcggccgatg ttcgaccacg tgacgatccg agcagcggac
     3301 cgcgaggcct cggagcggtt gtacgccact gtgctcgccg cgctcgggat cgagcagagc
     3361 ggcagcatcg agatgctcgc cgagtgggat gacttctcgc tcgcgcaggc gagcggcgag
     3421 cggccgataa cgcgcggcct gcacatcggg ttcaccgcgc catcacgcga gcacgtggac
     3481 gagttctggg aggtcggcat ggcagccgga ctccgcgacg acggtgagcc ggggcctcgt
     3541 cccgagtacc gctacgacta ctacggcgcg ttcctgctcg acccggacgg caacagcatc
     3601 gaggccgtgc accacggcgg cgtgcggcag gacgggaaca tcgatcacct ctggatacgc
     3661 gtggcggacg tcgccgcctc gaggcgcttc tatgacgtga tctccccgca cgccggctac
     3721 gagctcggtc acgagcatga cggtcgcgcg atgtttcgcg ggccgagcgg ctcgttcacg
     3781 gttctccgcg gcgagccgac cgagaatctg cacatggcgt tcccggcgga caccaacgac
     3841 gtggtggagg cgttccacac cgcggcgacg ggcgccggct acctcgacaa cggcggaccg
     3901 ggcgagcgcc ccgtctacca cccgggctac tacgccgcct tcgtgctcga ccccgacgga
     3961 aacaacatcg aggtcgtgaa ccacaaccgc gcgcggtagc tttccagtcg cgccctgttt
     4021 cgacttctgg gaaggagcct cccatggcca aggtgctcat cctcaccggc gatgccgctg
     4081 agtccctcga ggtcatgtac ccgtaccagc ggctgctcga ggaggggtac gaggtggaca
     4141 tcgccgcccc ctcgaagaag aagctgcagt tcgtggtcca cgacttcgtg cccgagttcg
     4201 acacgtacac ggagaagctc ggctacacct gggacgccga cctcgcgttt gccgacgtcg
     4261 atccgtcgca gtacgtcgcg gtagtggtcc cggggggccg cgcgcccgag tacatccgca
     4321 acgacgagga cttcaagcgg atcgtgaacc aattcttcga cgacaccaag ccggtcgcac
     4381 agctgtgtca cgcaccgctc gcgctggccg cggcaggcgt tctgcgcggg cgccggacgg
     4441 cggcctatcc ggcacttgcg ccggacgtgg aggccgcggg tgccgagttc gtggacggcg
     4501 acgcggtggt ggacggcgtg atggtgtcgg ctcgcgcctg gccggaccat ccctcgtgga
     4561 tgcgcgagtt catccgggtg ctgcgtgagc gcgcgccggc gggcgcgccg caggccgcgg
     4621 gcgtcgccta gcgagcccgg gcctcagcga gcgcacgggg ttcgcccctg aatccagtgc
     4681 gtctcggacc ggtggcgttt tgctgcgcgg acgagggtga gggccttatg tcgtgggctc
     4741 ttccctcgtc gcgctgctga gcgtcatcgc cgggctggcc ggccaccacg attccatgcc
     4801 gcggcacacg ggctacgtcg tggcgcacgt gcgcgccggc cgctcgctcg agcttcgcgc
     4861 gtcgcccggc ggccgctcgc tcctgcgagt aggtgaccgc accgagttcg gcacgcgcaa
     4921 gacgctcggc gtaacggcgc gcaggcacgg ctggctggcg gtcaccgatc cccgtctagc
     4981 gaacggccgc cgtggctggc tgagagcggg cgatccccgc gtcgagctca cgcagaccga
     5041 ggtggcgctg cacgccgaca tctcgcgtcg caagctcacg ctcgagcgcg acggccgcac
     5101 cgtcgaggaa gctcgcgtgg cggtcggggc tcccagctcg ccgacgccgg ccggccggtt
     5161 cgcgatcacg gacaagctgc gcggcccgga ctacagcgct tcctacggct gctgcatcct
     5221 cgcgatgtcc ggcagccagc cgcgcacgcc ggcgggctgg cggggcggca acaggcttgc
     5281 gatccacggg acgagcagct ccgagacgat cgggcgggcg agctcggccg gctgcctgcg
     5341 caccgacgac cgctcgctca ggaccatgat gcgcaaggtc cccgtcggcg cgcccgtgtt
     5401 catccaccgc tagcgcgacg ccacccgtga cttaagcgct aggcacgtgt tacggcaatg
     5461 acagctactt gaaaggagtg tccgctcatg tggatctcca agcgatcgct ggtgatcggc
     5521 ctcctggccg cgatgacagc gctcggcatg atgctggccg tcggcgcctt cggcgcggca
     5581 ggtggaggga aggcggccaa gcacagccgt ccgggcagcc agaaacacgg taagcgtcac
     5641 ggcgcaccgc tcatcaagga gtcgctcgca ccgtcacagc cgactgaccc cgcgttccac
     5701 ggtgtgagcc cgggcgctgc gccgtgggtg ttgaagcgcg gcgaggtgcg gctgaagagc
     5761 gacggcaagc tcaccctccg ggtgaagggg ctcgtgatcc ccatcgcgcc tggaaacggc
     5821 acgccgggcc cggtcaacac gatcagcgca tcgctctact gcggcgccga cacgaacacc
     5881 accgccacgg acacgacgga ccaagtgccg atctcgcgca agggtgacgc tcgcatcaag
     5941 gacaagacgt tcactgtgcc cgccacctgc ctcgcgccgg tgatcctcgt ccatccgaac
     6001 ggggacggga cgcactacat cgcagtcgac ggctggcgcc tctagtgcgt ctcagccgtc
     6061 atacgccgca tggcctcgtc ggtcggctgc ccctcgggct ccggctcggc gagcgccttg
     6121 cgcgcgttct cgagcccgcg tgagatcagc gagtcggcct tgctgaagtc ggtcggcagc
     6181 acgtcgagcg gccacggggg cgggagcacc accagccgca cccggtcgcg gtactcctcg
     6241 acctcgcgcc gcaggcgctc gtgcagcagg atcgtcgcgg cgtgcacgcc gatcgctagc
     6301 gcgcccgccg gcggctcgtc gatctccgtg ctcattcccg tgggcagcac gtagatctcg
     6361 tcgcaaccgc gctcgaccgc gcagctgatg ggcgtgttgt cggccacgcc gccgtcgacg
     6421 caaagccggc cgtcgatctc tacgggcggg tagatgcccg ggatggcggc ggtggcggcg
     6481 agcgcgtctt ccgcgctgcc gttttcgacc agtttctcct ggccgcatag gaggtcggtg
     6541 acgacgatcc cgagcggcgt gtcggtggcc tccagccgtt cgatgtcgag ctgccgctcg
     6601 atcaggcgac gcaggccgcg cgggctcacg aggtgattgc ggcgtccgag caggcccagc
     6661 accaccgtcg ccgggctgaa agggaacacc tgccgccgcg tgacgccgcg ccacgtgtcc
     6721 tgaagcttgt gggccgatgc gacctcctgc ggattggtgg cgaggaagcc ggcgttgagc
     6781 ccgccggccg acgtcccgac catgtaatcc gcgcggatcc cgcgctcgta gagggcctcc
     6841 gccatgccgg cctcgatggc gccgagcgac gcgccgccgg cgagtacgaa cgcggtggac
     6901 atgcgtcgaa tctagccgcg atgatcaact ctcgatcccg agcccgaggg aggcttgatg
     6961 gccaaccagt cgagagccgt cttgatcacc ggttgctcct ccggcatcgg tcacgccacc
     7021 gccgaccgcc tggcgcgagc cggcaagcgc gtgtacgcga ccgcgcgcag gccggacgcc
     7081 atcgctgacc tcgcagagag cggctgcgag ctgctcgctc tcgacgtgac cgacgaggag
     7141 tcgacgcgca gcgctgtgga gcacgtggag gagcgcgaag gcgccgtagg ggtgctcgtg
     7201 aacaacgctg gctatagcca gtccggggcg atcgagtcgg tgcccatgga ggccgtgcgc
     7261 cgccagttcg aaacgaacgt gttcggcctc gtgcggctca ctcagctcgt actgccggcg
     7321 atgcgagcgc aacggtgggg gaggatcgtg aacctcagct cgatcggcgg tcgtgtggtt
     7381 tttccgggcg gcggcttcta ccacgcgacc aagtacgccg tggaggcgat ctcggacgcg
     7441 ctgcgcttcg aggtcgaagg attcggcatc ggcgtgaccg tgatcgagcc gggtctgatc
     7501 agaacccgct tcggggaggt ggcggcgagc tcggtgcgcg acgagaccgc gggccgcggc
     7561 gcctacgacg atttcaacga ggccgtggcg accacgaccg ccggcgcgta cgcgaacggc
     7621 ctcctggcga agttcagcgg cacgcccgac gacgtggcca aggcgatcga gaaggcgatc
     7681 gacgcggagc gcgcacccac gcgcgtgccg gtcacggcgt ctgcgcacct ggtgatcccg
     7741 cagcgaaaga tcacccccga tcgcgtgtgg gaccggatga tgcgcgtcgc ctttccgcgg
     7801 ccgaagtagt cagaccgcgc ggcccgaagc catcacgaag tccggcgcgg tgaacttgcg
     7861 ccgcgggtca gacagcaggt cctgtgcggc gtcgatctcg aagcggtcga cctcggttag
     7921 ccgcgggcgc gcgcgttcga tcgccttcag cgtgatgatg cacgggagcg agccgcccgg
     7981 cacgtagcgc gacacggtct ccgcttgcac ctcgagcagc ccgtttcgct cgagcgcctc
     8041 gggcagctgc ttgccgtagg ccgggtccca gcccgactgc gccatgccgc gcatgaacgt
     8101 gccccagacg cggtcccagg cctcgtccgg ctctacgggc ccgtggacgg tgaagtcgag
     8161 ctcgcccagc gcgaggatgc cgtcgggcgc gaccgcgtcg atcatccgcc gcagcacctc
     8221 gaacttcgac ggcaggctgc cgagcagcac gcggctgtgc acgagctcgt acggcccgtc
     8281 ggggagcggc tccggtgccg cggcatcgtg ctttcgcacc tccacacgtg tgccctgcaa
     8341 gtgcttccgc agccgctcgt cgttcacgtc gagcgccacc acgcgcccat tcggcgccac
     8401 ctggtgtgac atccagcgcg caatcgtgcc gtcgccggcg cccacgtcga ggcagcgcca
     8461 gccctcgtcg atgccgagcg cgccgaggta cgccgtggtg aagcggtcgt gatcgtcggt
     8521 gaagtcgctc aaactgccgc cgtctggtcc cggcggagaa gctagcgaga ggccaggctg
     8581 tctagcggtg cgactcgatc tcgaccgcct gctggaaggt cggccggttg atccagtgga
     8641 tgagcggctg ggtgacgccc ccgagcggac gttggcggac ggcgtcgtag caccactggt
     8701 tgccgtcctt cttctccgcc ttgcacacct cgtcgccgct gtagatcttc gaggcgggcg
     8761 tgttcaccgc ctggcgcagc gtcgaggcaa gcagcgcccg gcagcgcgtg accttcccgc
     8821 ggccgcagta gacgcgtgag tacggcccct tcaccttctt gcccgagatc gtgcgcaggt
     8881 ccttgagcgc atagccgtac cagcccgcct ggtacgccga gccgaggtgg tcgccgtggt
     8941 tgttcggagc gttgtcgatc tcgttcacct tcgctatcgc gtcgaacagc tccttgccga
     9001 gcaccggctc gaactgtgcg tggatccaga gcggccacca ggcgtccatg attcgcactg
     9061 cctcggcctg gtcgtacacg ccgtcgcggt tcttgtcgcg gcggtgggct ccacccttcg
     9121 cccactgggt gaggatgttg atcgcgttct gcagatgcgg gtccttctgc ggcccgagca
     9181 ctcgcagcat ccagggcagc acggcgttgc ctcgcaggtc gaccgtgccc gcctcctcca
     9241 tggcgtcgat taggccgggc agattgatct tcttcgcccc gcgaatgcgc gtcttgatgc
     9301 cgtcgtcgag cggcttcgag cggtacaccg actggaacga gaagttgtcg tcggcggccc
     9361 gatagccgcg cgcctgcgtg ttgttccagc tcgtgatgta gctctggtcc accacctgcg
     9421 gatgggcgct ctgcggcgtg tagtcggcgg tcaggtcggc cgggttgaag ttgcgccact
     9481 cgaaccgctt caggcccatg gtggggaagt tcgtgtcgat gcccttcggg cgcaccgggt
     9541 tgttccccga gttgaagtac gcgtcgtgct tgtcgtccac gtacagccag ttgaacgtgt
     9601 agccgatctt gtacgccgcg ttctggaagt cctgtggcgt gcgcatcttg tcggggttgt
     9661 tgaagtcgga gaagccgagc gccgaatcga cctcgtgcat gtaggtcgag cgcagcttcg
     9721 agtacacgac cggcttcccg cgcacggtgc ccttgccggc gacgatcccg agcgcggtgc
     9781 gctgcgcgtg cagcgtctcg ctgccggccg gggtctggtc ggcggcgttg ggctgccacg
     9841 agttcgtgcg atcgagcttc tcgatctggc ggcacgagcc gtggaagcgg taatggtcgg
     9901 agtcgagggt cggcttcgac ccgtccggat tgcacagcgg cagcgcgaag gtgtcgatga
     9961 tgtcctggcc cgccgaggtg gcgctccagg cgtaatcgcg gccgcggccg agctcgacgt
    10021 agaggttcgt gcccgggaag cccgcgccgc gtgcgtcgat cgtgggcgcg tgcacgtcct
    10081 ggtccatcag gatctgtggc gcgaaatagc ccgtctgcgg gccgaacacc gcgagcgggc
    10141 gccccgactg cgactccgcg cccgacac
//
LOCUS       DASVXS010000059         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_296832, whole genome shotgun sequence.
ACCESSION   DASVXS010000059 DASVXS010000000
VERSION     DASVXS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296832"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VF752_03290"
     CDS             complement(<1..1211)
                     /locus_tag="VF752_03290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8854599.1"
                     /translation="MTSERFSIAQVTPYAWEQHHEVNRYVERLSDELCRRGHRVVVIA
                     PSDSRVLIRESRKLVRALREDPDAVFASEGCAQVVAVGQSIPFPPARRGGAVSLPVDV
                     SRTIEQLLETASFDFVHVHEPFAPSAASTALRHSRALNVGSFHSPTERVMSTQVARRL
                     VELLFGRLDGRSASFPTTRELVARYFPGDYELVPPGADVVECEKSGEPVEIFFSLEEE
                     RSALRVFLRALRRLPEDLDWRAMVWSRLPSAAPAVPLSKALRERVAFRSQADGSEASH
                     LARADILVAGSAGASPAPQLVLRGLAGGAVPVVSRLRVYEDALSDGELGLLFEPRDAP
                     MLATQLERLIRDEPLRRGLHDEARRRRDELGWDRTAERFERLYGEIAARRHSPDGAPA
                     IRNRLARRDFI"
     gene            complement(1255..>2383)
                     /locus_tag="VF752_03295"
     CDS             complement(1255..>2383)
                     /locus_tag="VF752_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854600.1"
                     /translation="TEKVRVAIVGVGNCASAFVQGVQYYKDADPAKSVPGLMHVDLGG
                     YHVRDIEFTAAFDIDADKVGKDLSEAIWSGQNNTIKFADVPKLGVKVHRGMTHDGLGK
                     YLKEKIQKAPGETADIVQILKDTKTDVVVSYLPVGSEQATKWYVEQVLEAGCGFVNCI
                     PVFIAKEEYWSKRFKKAGLPIVGDDIKSQVGATIVHRQLARLFGDRGVNIVHTSQLNV
                     GGNMDFYNMLERERLESKKISKTNAVTSVMHNELPADDVHVGPSDYVPWLTDRKWAHI
                     RVEGQAFGDVPLNLELKLEVWDSPNSAGIVTDAVRCCKLALNHGIGGPLEEPSSYLMK
                     SPPVQVPDSIARENTEKFIAKYGGAPKLPGSKPKVRTTEKS"
BASE COUNT          357 a          833 c          820 g          373 t
ORIGIN      
        1 tggatgaagt cgcggcgcgc caggcggtta cggatcgctg gagccccgtc aggtgaatgc
       61 ctccgcgcgg caatctcgcc gtagaggcgc tcgaaacgct ctgcggtgcg gtcccagccg
      121 agctcgtcgc ggcgacggcg ggcctcgtcg tggaggccgc ggcggagggg ctcgtcccgg
      181 atcaggcgct cgagctgggt ggcgagcatc ggtgcgtccc gcggctcgaa cagcagcccg
      241 agctcgccgt cagagagcgc gtcctcgtac acgcgcaggc gcgagaccac tggcacggcg
      301 ccgcccgcca ggccgcgcag cacgagctgc ggcgccggcg aggcgccggc ggagcctgcg
      361 acgaggatgt ccgcgcgcgc caggtggctg gcctcggagc cgtcggcctg cgaccggaac
      421 gcgacgcgct cgcgcagcgc cttagagagg ggcacggccg gagcggcact cggcagccgc
      481 gaccagacca tcgcgcgcca gtcgaggtcc tcgggcagcc gccgcagcgc gcgcaggaag
      541 acgcggagcg cgcttcgctc ctcctcgagc gagaagaaga tctccaccgg ctcgccggac
      601 ttctcgcact ccaccacgtc cgcgccgggc ggcacgagct cgtagtcacc cgggaagtag
      661 cgggccacca gctcgcgcgt ggttgggaag ctggcgctgc gcccgtcgag gcgcccgaac
      721 agcagctcca ccagccgccg tgccacctgc gtcgacatca ctcgctcggt gggcgagtgg
      781 aagctgccca cgttgagcgc gcgcgaatgg cgcagggccg tcgacgccgc gctcggggcg
      841 aacggctcgt gcacgtgcac gaagtcgaag ctggcggtct cgagaagctg ctcgatcgtg
      901 cgcgagacgt ccacaggcag cgacaccgcc ccgccccgcc gagcgggcgg gaaggggatg
      961 ctctggccga cggcgacgac ctgggcgcag ccctccgacg cgaacaccgc gtccgggtct
     1021 tcgcgaaggg cgcgcacaag cttgcgcgac tcgcgtatca gcacacgcga gtccgacggc
     1081 gcgatcacca ccactcggtg cccgcgcctg cacagctcgt ccgacagccg ctccacgtag
     1141 cggttcacct cgtggtgctg ctcccaggcg tacggcgtga cctgcgcgat cgagaagcgc
     1201 tccgacgtca cgcgcacaga ctagaagggg cgccccgcag ggcgccccct cgcgctacga
     1261 cttctcggtc gtcctgacct tcggcttgct gcccgggagc ttcggtgcgc cgccgtactt
     1321 cgcgatgaac ttctcggtgt tctcgcgagc gatcgagtcc ggcacctgca ccgggggcga
     1381 cttcatcagg tacgagctcg gctcctcgag cgggccgccg atgccgtggt tcagcgcgag
     1441 cttgcagcag cgcacggcgt cggtgacgat gccagccgag ttcggcgagt cccacacctc
     1501 gagcttcagc tccaggttga gcggcacgtc gccgaaggcc tggccctcga cgcggatgtg
     1561 cgcccacttg cggtcggtga gccacggcac atagtcggag ggacccacgt gcacatcgtc
     1621 ggcgggcagc tcgttgtgca tcaccgaggt gaccgcgttg gtcttcgaga tcttcttcga
     1681 ctcgagccgc tcacgctcga gcatgttgta gaagtccatg ttgccgccca cgttgagctg
     1741 cgaggtgtgg acgatgttca cgccgcggtc gccgaacagg cgggcgagct ggcggtggac
     1801 gatggtcgcg cccacctgcg actttatgtc gtcgccgacg atcgggagtc cggccttctt
     1861 gaagcgcttc gaccagtact cctccttggc gatgaacacc gggatgcagt tcacgaagcc
     1921 gcagccggcc tcgagcacct gctcgacgta ccacttggtc gcctgctccg agccgaccgg
     1981 gaggtaggac acgaccacgt cggtcttcgt gtccttgagg atctggacga tgtcggcggt
     2041 ctcgcccggc gccttctgga tcttctcctt gaggtacttg ccgaggccgt catgcgtcat
     2101 gccgcgatgc accttcacgc cgagcttcgg cacgtcggcg aacttgatcg tgttgttctg
     2161 gcccgaccag atcgcctcgg agaggtcctt gccgaccttg tccgcgtcga tgtcgaaggc
     2221 ggcggtgaac tcgatgtcgc gcacgtggta gccgcctagg tccacgtgca tcaggcccgg
     2281 cacgctcttg gccgggtctg cgtccttgta gtactgcacg ccctgcacga acgcagaggc
     2341 gcagttgccg acgccaacga tcgccacccg gaccttctcg gtc
//
LOCUS       DASVXS010000060        12716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_439448, whole genome shotgun sequence.
ACCESSION   DASVXS010000060 DASVXS010000000
VERSION     DASVXS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12716
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439448"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF752_03300"
     CDS             complement(<1..212)
                     /locus_tag="VF752_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854601.1"
                     /translation="MGRFRVTVRRGPKVEHEKHDSLAAALDSIQVRARELSEDADAHT
                     LDTKLGRKFEPVQQVVGRIELRGPGVP"
     gene            265..591
                     /locus_tag="VF752_03305"
     CDS             265..591
                     /locus_tag="VF752_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854602.1"
                     /translation="MSTLVIILIAVGVLVLLLLVAQVMRRRREQKALMRERLTGTLEG
                     HHQQVEANSAKADELRPQAEAHRRAAEQHAEQAEELDARADRAERAAAFHDDRADETR
                     QEIEKI"
     gene            complement(598..1593)
                     /gene="moaA"
                     /locus_tag="VF752_03310"
     CDS             complement(598..1593)
                     /gene="moaA"
                     /locus_tag="VF752_03310"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX8854603.1"
                     /translation="MKREPLFDGHGRRIADLRVSVTDRCNFRCQYCMPAEGLPWLERS
                     DILQFEEIERLVSLMAGMGVTDVRLTGGEPLVRRDFPKLVAMLARIDGVEDLSLTTNA
                     YLLERDAAALVDAGIQRVNVSIDSLQRDRFFQLTRRDALPQVLRGLDALAAHPEVHPI
                     KVNAVAMRGFTEQEALPFADFARSSAFQVRFIEFMPLDADHAWSPDAVLSGDELRRII
                     HAVYPLEELPREPSSTARVFRFADGRGEIGFINPVSEPFCADCNRIRLTADGKLRTCL
                     FSLHETDLRTPLREGADDVELERIVREAVWRKELKHHVGEPGFRQPARTMSSIGG"
     gene            1645..2655
                     /locus_tag="VF752_03315"
     CDS             1645..2655
                     /locus_tag="VF752_03315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8854604.1"
                     /translation="MSAGNETKGIRMERRRLRDLEVSAEGLGLMGMSEFYGPGDDDES
                     IKTISRALELGIDFFDTADMYGYGANERLAGKAIADRRDQVVLATKFGNVRDPDNPER
                     RGIDGRPEYVHQACNASLERLGTDYIDLYYQHRVDKNTPIEETVGAMAELVQEGKVRH
                     LGLSEASPETLRRANAVHPITALQTEYSLWTRDPEDEILPTVRELGIGFVPYSPLGRG
                     FLTGQIQSVDDFAEDDFRRYNPRFQGENFQRNLDLVDRVREIAEEKGCTAGQLALAWV
                     LAQGDDIVPIPGTKHVRYLEENAGAVDIELTGDDLRRIDEVAPVGVAAGERYPDMSSI
                     DR"
     gene            2652..3500
                     /locus_tag="VF752_03320"
     CDS             2652..3500
                     /locus_tag="VF752_03320"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX8854605.1"
                     /translation="MRIGIVGGGLMGSGIAEVSARAGVDVTLIEVDDAAAERARKSIE
                     RSLERAVGSGKLQAPERDEALSRLVYSSDLGDIAGADAAIEAIIESESAKQQLFRELD
                     RLLPDAEFLASNTSSVPIMKLAAETTRPERVLGLHFFNPVPVLKLVELVRSLMTSDET
                     LIRARSFAEGQLGKTTIESKDRAGFIVNALLVPYILSAIRMYESGFASKEDIDRGMVL
                     GTAHPMGPLALADLVGLDTLLYVANSLYEEFREPSAVAPALLQRMVEAGLLGRKSGRG
                     FYDYTS"
     gene            complement(3601..4938)
                     /locus_tag="VF752_03325"
     CDS             complement(3601..4938)
                     /locus_tag="VF752_03325"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX8854606.1"
                     /translation="MADEQTQGAGGDSSVATAQDTLTVTDNRTGESYDLEIKNGTIRA
                     MDLRQIKVTDDEFGMMSYDPAFTNTASCRSEITYIDGEAGILEYRGYPIEQLAESSSY
                     LEVAYLLIHGELPTREQLDGWMHEITHHTYVHENVKKFMEGFRHDAHPMGMLLGTVGA
                     LSTFYPDAKDTKDEGERHMAAVRLIAKMPTLAAFSYRHNLGLPYIYPDNDLSYPGNFL
                     GMLYKMTEVKYEPDPRLERALDVLWILHADHEQNCSTSAVRTVGSSEVDPYSAVAAGV
                     AALYGPLHGGANEAVLRMLERIETKDNIPDFIKGVKNRDEKLMGFGHRVYKNYDPRAK
                     IIKRHADEVFEVTGKNPKLEIAVELEKYALEDEFFVERKLYPNVDFYSGLIYEAMNLP
                     KDMFTVMFAIPRTSGWLAQWLEMLEDEETKIARPRQVYTGQRERDYVPIDQRS"
     gene            complement(5176..5409)
                     /locus_tag="VF752_03330"
     CDS             complement(5176..5409)
                     /locus_tag="VF752_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854607.1"
                     /translation="MNLFVVFGPIFAAWALILTFALGLRSPHFPSTLRAERIVIGISL
                     VLFVCAVGSAILTASHKIGERKGPEKEKTALVR"
     gene            5526..6503
                     /locus_tag="VF752_03335"
     CDS             5526..6503
                     /locus_tag="VF752_03335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854608.1"
                     /translation="MDANVRAYAGVKKRRPNVALVVVTRDRPDLLARYTLPSLERISC
                     EPAEIVIVDQSAGPDTYELVSDLPGVRVLRSAPGLSRGRNLAVAETDSPFIVFTDDDV
                     EFGPGWLERVERLFDEPTVGAVCGRGVDSLGRPLPFKPAGTYRYPASPFGLGHGFNVA
                     FRRAALEQVGPFDERLGAGAPVPAAEDTDMLYRVMKAGWAARCDDGITVRHHSWRSAE
                     QERAAHRNYGVGFAAQTAKHLRKRDLAAARVAASEVAGHLFWLAVSAARGDRSALVLQ
                     LSWGQGAFAGARVLRQGLPSVPPPPEPLPLEPSREDGRFRRTMSARQAL"
     gene            6523..8133
                     /locus_tag="VF752_03340"
     CDS             6523..8133
                     /locus_tag="VF752_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX8854609.1"
                     /translation="MFDRRIPVRQQDVAGNIQEAPPEGASEFAERTTGLTVLRSSAWN
                     AASLILPQLYVVAMSVMAARFLTPAEMGRQSYIAFIALSLVMLMTGGVPAAVQRFGSE
                     ALGRGHVQHLLPLVRWAARIEVVGAALGAGIMIGSAVLGSGPQAAWVFAGVYCAFGIL
                     QTVPAVVLAATQRWRGVSILAIVSGAVATVVTLVVLALGGGITGMFVVEAIMMAFNLA
                     ITARLARSAVRGLNTEPVESRELRRRTARWALIATFSGFLAFVVWRRSEFLFLNELSS
                     DSQIAVYSIAFAAINAITRLPEAAGMVVSPAFATLFGARQMARIHTGYARAIRLILLG
                     ALPLTAAIIALGPIATRLVYGSSYSEAGTLMMIMAPALPLVALVSVSRGLISGLGLQR
                     VLVTVGVFAALLNVTLDLLLIPPYDATGAAIANATAQGAAAGAYVCFARQALGPIAWE
                     RSTLLRNCVAAIAAGAAGWGVAAALDGVAGLFVGLLACLSIYTGLAIALRVVPSPDRA
                     WLDELLEHRLKGRPEVLARQMLSAASRR"
     gene            8139..9965
                     /locus_tag="VF752_03345"
     CDS             8139..9965
                     /locus_tag="VF752_03345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854610.1"
                     /translation="MRVVLISREHPSRTSGGGIGTYTMTIGPALAGRGHDVEVLTEGP
                     GEEHVEHGVHVVPLEHPSLPPRTASRLLTARQVAHAARRDGAEVVQAAEWEAEAWWIA
                     RTGGSPLVTRLATPTYLVDMLNLGAQRDQSDLVRRMERDQALRSQAIVAPSASIADRV
                     AADWRLDRARIDVIPNPIDGAAVRSAGAADPPVSLPPRYLLFIGRIERRKGVDVLAAA
                     LPKALAAHPELHAVFLGRDAGAAGGDAGRRLDDLLEAFPGRVHRPGALPREQALPVVA
                     RASLVVLPSLWEAFGFVAVEAMALGRPVIASSGSGFEEIIEHDRSGWLVPPGDAPALA
                     EALVERAGDAVELDRISRGARVRADDFEASRIAPMLERVYERAIERADSPGHFTDAIY
                     SRGYRRFFRPEDSGNPFRQIYDEKLEAVVGRLRAMPSMRILDVGCGPGRALAPLALRH
                     QMTGCDVSAEMLEEARRRCPSHVELVRADARSLPFADESFDAVIALDLLTHLPDLRAG
                     VRELARVARSGGPVIFDTSNAEPWWVLAYPRYVNWRPRRLIATMRRGGVLPEWSEIVR
                     HHRADEARAAIEDAGLAIEDVGTFGPRWTPKWHLWYTRKGLP"
     gene            9962..11971
                     /locus_tag="VF752_03350"
     CDS             9962..11971
                     /locus_tag="VF752_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854611.1"
                     /translation="MSALPRAPLLFYLARGDDPQRLAVGPALAAAAGRAGWQFECYYA
                     EARRGRHYGGGDPSGARAQLAAGSLFAGGMHAERAFRIAVGYEVAALGDPGSPLWPVL
                     DEAGGAALAQTEDPVELYVAALAALVQDVPDMVLVLDAAPQGPHRVVTAPYLYPALLA
                     GEPAWALEASAGDDQRTALERLGVRHFRGLGVDGERALDFPGSVEPGDVFPVDDGWAA
                     FTVAAARTHRAWGRGVLLGDPALVAAQLPRARRLRLLPLHGRPQTDAIDAASDIVSEA
                     REPVFGRQWDDRDFFALASAGHGLQVVDPGPPFDSEAGIAARVPEPPEDEAAPEPGDD
                     ELLRWANEGRVLVTLLLWTGMVRELDCIPRLIDLAMATDLRAGLVLTAESYEQGGAHT
                     LGPLGVPVARGGVLGQIEPLLGSTGRGVAAEAYLGGGRLRGYLTEALAAIDARAPRAL
                     RPRGWWPLLDARLVRRRELAVGRRGRRPVARFNPRAEASPVGAGASSESTKPRRDLRG
                     IAGSAIRRTGLDSFLEERRPFDGWRPGVLDEGVASAVHGAGLEYMWTKTAFGRPRVVL
                     QDGDFVALPFTAGSWDGWSPFYTVGSHHGLLRAERRLRRHGEPGWLATTIDSPLFAMS
                     GEVLEHGAALHEIARVAAQGGSSGELVNVTPNVVARYARLLAPRG"
     gene            11979..>12716
                     /locus_tag="VF752_03355"
     CDS             11979..>12716
                     /locus_tag="VF752_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854612.1"
                     /translation="MIDLTWPRARPPALIAVSGIDGSGKTTLIDALRERLGEDGYKVE
                     VVWTRLEWTTLWESSSRLERITALVDWVPARKETAPAAPEPAEPTSAWLEPAPARSAA
                     AQLRRRSRLLTRAWAGVVATLHAQAQRKAVVPHLREGKVVICDRYTLDAAVALHRRYD
                     SVRSLGLQVRLMQFLSPRPLLAWHLDLPVAVAAQRKEHELGIAERQRENVLYRRERER
                     LGWSRLDAARPVDELVQQVTREAESALA"
BASE COUNT         1863 a         4308 c         4537 g         2008 t
ORIGIN      
        1 ggcacacccg ggccgcgcag ctcgatgcgg cccacgacct gctgcacggg ctcgaacttc
       61 ctgccgagct tggtgtcgag cgtgtgcgca tcagcgtcct cggacagctc gcgagcgcgc
      121 acctggatcg agtcgagcgc agcggcgagc gagtcgtgct tctcgtgctc gaccttcggg
      181 ccgcgcctca ccgtgacccg gaatctcccc acgcccggca acctagcggg ttatcgacga
      241 gccgcaagcg ggaacgcatt gggcatgtcc acgcttgtga tcattctcat agccgtaggc
      301 gttctcgtgc tcttgctgct tgtcgcgcaa gtgatgcgca ggcgccgcga gcagaaggca
      361 ctcatgcgcg agcggctcac cggaacgctc gagggccacc accagcaggt cgaggcgaat
      421 agcgccaagg ccgacgagct gcggccgcag gccgaggcgc accgccgcgc agcggagcag
      481 cacgccgagc aggcggagga gctcgatgcg cgggccgatc gtgccgagcg ggcggcagcg
      541 ttccacgacg accgtgcgga cgagacccgg caggagatcg agaagatcta gcggcgctca
      601 gccgccaatc gagctcatcg tccgagcggg ctggcggaag cccggctcgc ccacgtggtg
      661 cttcagctcc ttgcgccaca cggcctcgcg cacgatccgc tcgagctcca cgtcgtcggc
      721 gccctcccgc agcggcgtgc gcaggtcggt ctcgtgcagc gagaacaggc acgtgcgcag
      781 cttgccgtct gcggtgaggc ggatgcggtt gcagtccgca cagaatggct ccgagaccgg
      841 gttgatgaag ccgatctcgc cacgcccgtc ggcgaaccgg aacacccgcg ctgtcgagga
      901 cggctcacgc ggcagctcct ccagcgggta gaccgcgtgg atgatccggc gaagctcgtc
      961 gccgctgagc acggcgtcgg ggctccacgc gtgatccgcg tcgagcggca tgaactcgat
     1021 gaagcgcacc tggaacgccg acgagcgagc gaagtccgcg aacggcagcg cctcctgctc
     1081 ggtgaagccg cgcatcgcta cagcgttcac cttgatcggg tgcacctcgg gatgcgcggc
     1141 gagcgcatcg agcccgcgca ggacctgcgg gagcgcgtcg cgccgagtga gctggaagaa
     1201 gcggtcgcgc tgcagcgagt cgatcgacac gttcacgcgc tggatgcccg cgtcgaccag
     1261 cgcggcggcg tcgcgctcga gcaggtaggc gttcgtcgtg agggagaggt cctccacccc
     1321 gtcgattcgc gcgagcatcg cgaccagttt cgggaagtcg cggcgcacca gcggctcgcc
     1381 gccggtgagg cggacgtcgg tcacgcccat ccccgccatc agcgacacca gccgctcgat
     1441 ctcctcgaac tggaggatgt cgctgcgctc gagccacggc aggccttcgg ccggcatgca
     1501 gtactggcag cggaaattgc agcgatccgt gacggacacg cgcaggtcgg cgatgcggcg
     1561 cccgtgtccg tcgaagagcg gttcgcgctt cacacgactc aggttacccc tggccgcccc
     1621 gccgaatcca ggccgtttgc cacgatgtca gcgggcaacg agacgaaagg aatccgcatg
     1681 gagcggcgac gactgcgcga cctcgaagta tcagccgagg gcctagggct gatgggaatg
     1741 tcggagttct acgggcccgg cgacgacgac gaatcgatca agacgatcag ccgcgcgctc
     1801 gagctcggga tcgatttttt cgacacggct gacatgtacg gctacggcgc gaacgagcgg
     1861 ctggcgggca aggcgatagc cgatcggcgc gaccaggtcg tgctcgccac caagttcggc
     1921 aacgtgcggg acccggacaa ccctgagcgg cgcggcatcg acggccggcc ggagtacgtg
     1981 caccaggcgt gcaatgcctc gctcgagcgg ctcggtaccg actacatcga cctctactac
     2041 cagcaccgcg tggacaagaa cacgccgatc gaggagaccg tcggcgcgat ggccgagctc
     2101 gtgcaggagg gcaaggtgcg ccacctcggc ctctcggagg cctcgccgga gacgctccgc
     2161 cgggcgaacg ccgtccaccc gatcacggcg ctccagacgg agtactcgct ctggacgcgc
     2221 gacccagagg acgagatcct cccgacggtg cgtgagctcg gtatcggttt cgttccgtac
     2281 tcgccgctcg gccgcggctt tctgaccggg cagatccagt cggtagacga tttcgcggag
     2341 gacgacttcc gccgctacaa cccgcgcttc caaggtgaga acttccagcg aaatctcgac
     2401 ctcgtggacc gcgtgcgcga gatcgccgag gagaaaggct gcacggccgg acagctcgcg
     2461 ctcgcttggg tactcgccca gggcgacgac atcgtcccga taccggggac caagcacgtg
     2521 aggtatctcg aggagaacgc cggcgccgtc gacatcgagc tcaccggcga tgacctccgc
     2581 cggatcgacg aggtcgcgcc ggtcggcgtc gcggcgggcg aacgctatcc cgacatgtcg
     2641 agcatcgacc gatgaggatc ggcatcgtgg gcgggggcct gatgggctct ggcatcgccg
     2701 aggtgtcggc gcgcgccggc gtggacgtga cgctgatcga ggtggacgac gccgccgccg
     2761 agcgcgcccg caagtcgatc gagcgctcgc tcgagcgcgc cgtggggtcg ggcaagctgc
     2821 aggcgcccga gcgcgacgaa gcgctcagcc ggctcgtcta cagctccgac ctcggcgaca
     2881 tcgccggcgc cgacgcggcg atcgaggcga tcatcgagtc ggaaagcgcg aagcagcagc
     2941 tcttccgcga gctcgaccgg cttctcccgg acgcagagtt cctcgcgagc aacacctcgt
     3001 cggtgccgat catgaagctg gccgcggaga ccacgcggcc cgagcgcgtc ctcgggttgc
     3061 atttcttcaa cccggtcccg gtgctgaagc tcgtggagtt ggtgcgctcg ctgatgacct
     3121 ccgacgagac gctcattcgc gcccgctcgt tcgccgaggg ccagcttggc aagacgacga
     3181 tcgaatcgaa ggaccgcgcc gggttcatcg tgaacgcgct cctcgtgccc tacatcctgt
     3241 cggcgatccg catgtacgaa tccgggttcg cctcgaagga ggacatcgac cgcggcatgg
     3301 tgctcggcac tgcacatccg atgggcccgc tcgcgctggc cgacctcgtc ggcctcgaca
     3361 cgctcctcta cgtcgcaaac tcgctctacg aggagttcag ggagccgagc gcggtggcgc
     3421 cggcgctgct gcagcgcatg gtcgaagccg ggctgctcgg ccggaagtcc ggccgcggct
     3481 tctacgacta cacctcttag gcgtcgaaaa ccaagccagg caaggacgca gggccgaagc
     3541 ttggcgatag ccaagcgagt gcccgaggac ggcgcatggc gcaggtttgc aggcgtctag
     3601 ttagctgcgc tggtcgatgg ggacgtaatc gcgctcacgc tggccggtgt acacctggcg
     3661 cgggcgcgcg atcttggtct cctcgtcctc gagcatctcg agccactgcg ccagccagcc
     3721 cgacgtgcgc gggatggcga acatgacggt gaacatgtcc ttcggcaggt tcatggcctc
     3781 gtagatgagg cccgagtaga agtcgacgtt cgggtagagc ttgcgctcca cgaagaactc
     3841 gtcctcgagc gcgtacttct ccagctcgac cgcgatctcg agcttcgggt tcttgccggt
     3901 gacctcgaag acctcgtcgg cgtggcgctt gatgatcttc gcgcgcgggt cgtagttctt
     3961 gtacacgcgg tggccgaagc ccatcagctt ctcgtcgcga ttcttcacgc ccttgatgaa
     4021 gtccgggatg ttgtccttcg tctcgattcg ctcgagcatc cgcagcacgg cctcgttcgc
     4081 gccgccgtgc aacgggccgt acagggcagc cacgccggcg gccacggccg aatagggatc
     4141 gacctcgctc gacccgacgg tgcgtaccgc gctcgtcgag cagttctgct cgtggtcggc
     4201 gtgcaggatc cagagcacgt cgagggcgcg ttcgagccgc gggtccggct cgtacttcac
     4261 ctcggtcatc ttgtagagca tcccgaggaa gttgcccggg tagctcaggt cgttgtccgg
     4321 atagatgtac ggcagcccca ggttgtggcg gtacgagaag gcagcgagcg tcggcatctt
     4381 cgcgatcagc cggaccgcgg ccatgtgccg ctcaccctcg tccttggtgt ccttggcgtc
     4441 gggatagaag gtcgagagcg cgccgaccgt gccgagcagc atccccatcg ggtgggcgtc
     4501 gtggcggaag ccctccatga acttcttcac gttctcgtgc acatacgtgt ggtgcgtgat
     4561 ctcgtgcatc cacccgtcga gctgctcgcg cgtgggcagt tcgccgtgga tgaggaggta
     4621 cgcgacctcg aggtaactcg agctctcagc gagctgctcg atcgggtagc cgcggtattc
     4681 gaggatcccg gcctcaccgt cgatatacgt gatctcgctg cgacacgacg ccgtgttcgt
     4741 gaacgcgggg tcgtaggaca tcatcccgaa ctcgtcgtct gtgaccttga tctggcggag
     4801 atccatcgcg cggatggtcc cgttcttgat ctcgaggtcg tagctctcgc cggtccggtt
     4861 gtcggtgacc gtgagcgtgt cctgagcggt ggctaccgag ctgtcaccgc ccgcgccctg
     4921 tgtctgctcg tcagccatac atctcctccg tggcgacttc cgtcatttcc tctcacactg
     4981 gattgtgacg gacgcgcgtc cccgggtggt cccgggggtc ccattcgagc gctgcccgaa
     5041 agggcactca cacgtgcacc gcccgaaggt ccagtgcgaa cctagacctg cgggtggtgt
     5101 cggctcgtgc aaccaaggcg atagtggggc cccatgggtg agcctcagat gcacataccc
     5161 cggatcggat tgtgctcagc gaacgagtgc ggtcttttcc ttctccgggc ccttgcgctc
     5221 gccgatcttg tggctcgccg tgaggatggc gcttccgact gcgcagacga agaggaccag
     5281 ggagatgccg atcacgatgc gctcggcgcg cagcgtggac gggaagtgcg ggcttcgcag
     5341 ccccaaggcg aacgtgagta tgagggccca cgcggcgaag atcggcccga acaccacgaa
     5401 gaggttcacg ccgccggaga gtacctgacc gcacggcagc agggcggcgc gatttcgtgg
     5461 gtatgcgggg tttgagcgcg cggccccggg tacggggtgt cggacggatc tgaaggggag
     5521 tcggaatgga cgccaatgtg cgtgcgtacg cgggggtgaa gaagcgccgg cccaacgtcg
     5581 cgctggtagt cgtcactcgg gaccgccccg acctgctggc ccggtacacg ctgccgagtc
     5641 tcgagcgtat ctcgtgtgaa ccggccgaga ttgtcatcgt cgatcagagc gccggtcccg
     5701 atacgtacga gctcgtcagc gacctgccgg gtgtgcgcgt cctccggagc gcccccgggc
     5761 tctcgcgggg tcgcaatctc gcggtcgccg agaccgactc acccttcatc gtcttcaccg
     5821 acgacgacgt cgaattcggg cccggatggc ttgagcgcgt cgagcggctg ttcgacgagc
     5881 caaccgtggg agccgtgtgc gggcggggag ttgactcgct cggacgtccg ctgccgttca
     5941 agccggccgg cacctatcgc tatcccgcga gccccttcgg gctggggcac ggattcaatg
     6001 tcgcgttccg ccgcgccgcg cttgagcagg tcgggccgtt cgacgagcgg ctcggagcag
     6061 gcgcgccggt accggccgcg gaggacaccg acatgctcta tcgggtcatg aaggcgggat
     6121 gggcggctcg ctgcgacgac gggatcacgg tgcgacatca cagctggcgc agcgccgagc
     6181 aggagcgcgc cgcacaccgg aactacggcg tcggcttcgc cgcgcaaacc gccaagcacc
     6241 tgcggaagcg cgacctcgcg gccgcccggg tggccgcatc cgaggtcgcc ggccacctgt
     6301 tctggctcgc cgtctctgcg gcgcggggcg atcgctcggc gctcgtgctg cagctgtcct
     6361 ggggtcaggg cgcgttcgcc ggggcccgcg tcctgcgcca agggctaccc agcgtccctc
     6421 cgcctccgga accactgccg cttgaaccct cgcgcgagga cgggcgcttc agacgtacga
     6481 tgtcggcgcg ccaggcgcta tgacggaaat cgcatcggcg cgatgttcga tcggcggatc
     6541 ccggtgcggc agcaagacgt agcggggaat atccaggagg cgccgcccga aggcgcgtcg
     6601 gagttcgctg agcgcaccac cggcctcacg gtgctgcggt cgagcgcctg gaacgccgcc
     6661 agcctgatcc tgccgcagct gtatgtggtc gccatgtcgg tcatggcggc gcgcttcctg
     6721 acgcccgccg agatgggtcg gcagagctac atcgcgttca tagcgctgtc gctcgtgatg
     6781 ctgatgacgg gcggcgtgcc cgccgcggtc cagcgcttcg gaagcgaagc gctcgggcgc
     6841 ggccacgtgc agcacctctt gccgctcgtg aggtgggccg cgcgcatcga ggtggtcggt
     6901 gcggcgcttg gcgctggaat catgatcggc tccgccgtgc ttggctcggg gccgcaggcg
     6961 gcgtgggtat tcgccggcgt gtactgcgcc ttcgggattc tgcagacggt tcccgccgtc
     7021 gtcctcgcgg ctacgcagcg ctggcgcggc gtgtcgatcc tcgcgatcgt gtcgggcgca
     7081 gtcgcgacgg tcgtgacgct cgtggtgctc gcactggggg gcggcatcac cgggatgttc
     7141 gtggtcgagg cgatcatgat ggcgttcaac ctcgctatca ccgcgcgact ggcgcgaagc
     7201 gcggttcgcg ggctcaacac cgagccggtg gagtcgcgcg aactgcgccg gcgcacggcg
     7261 cgctgggcgc tgatcgcaac gttctccggc tttctcgcct tcgtcgtctg gaggcgctcg
     7321 gagttcctct tcctcaacga gctgtcgtcg gactcgcaga tcgccgtgta ctcaatcgcg
     7381 ttcgccgcga tcaacgcgat cacgcgcctg ccggaggcgg cgggcatggt cgtgtcgccc
     7441 gccttcgcga ccttgttcgg cgcgaggcag atggcacgga tccacaccgg ctacgcgcgc
     7501 gcaatccggc tgatcctgct cggggcgctt ccgctcacgg cggccatcat cgctctgggc
     7561 ccgatcgcga cgcggctcgt ctatggcagc tcgtacagcg aggccgggac gctgatgatg
     7621 atcatggccc ccgccctgcc gctggtcgcg ctcgtgagcg tcagccgcgg gttgatctcc
     7681 gggctcgggc tgcaacgagt gctcgtgacc gtgggcgtct tcgcggcgct gctgaacgtc
     7741 acgctcgacc tgctgctgat cccgccgtac gacgcgaccg gcgccgcgat cgccaatgcg
     7801 accgcgcagg gagcggcggc tggcgcctac gtgtgcttcg cgcggcaagc tctcggcccg
     7861 atcgcgtggg agcgcagcac gctgctgcgc aactgcgtcg cggccatcgc agctggggct
     7921 gcgggatggg gagtggccgc ggcgctggac ggcgtggccg gtctattcgt cggcttactc
     7981 gcgtgcctga gcatctacac cggacttgcg atcgcattgc gcgtagtccc ctcccccgac
     8041 cgcgcatggc tcgacgaact gctcgagcat cgcctgaagg gccggcccga ggtcttggcg
     8101 cgccagatgc tctcggctgc atcgaggagg tgagcgggat gcgagtcgtc ctgatctcgc
     8161 gcgaacaccc gagccggacg agcggtggcg ggatcggcac ctacacgatg acgatcggac
     8221 cggcgctcgc cggccgggga cacgacgtgg aggtgctgac cgagggcccg ggtgaggagc
     8281 acgtggagca cggcgtgcac gtggtaccgc tcgagcaccc gtcgctgcca ccacgaacag
     8341 ccagccgcct gctcaccgcc cgccaggtgg cgcacgcggc acgccgtgac ggtgcggagg
     8401 tggtccaggc ggccgagtgg gaggcggagg cgtggtggat cgcgcgaacg ggcggctcgc
     8461 cgctcgtgac gcgcctcgcg acgcccacgt acctggtcga catgctgaac ctgggcgccc
     8521 agcgcgatca gagtgacctc gtccggcgga tggagcgcga ccaggcgctg cgaagccagg
     8581 cgatcgtcgc gccgagcgcc tcgatcgccg atcgtgtggc ggcagactgg cgactcgacc
     8641 gcgcgcgcat cgatgtgatt ccgaacccga tcgacggcgc ggctgtccgc agcgccggag
     8701 ccgcggatcc tccggtctcg ttgccgccgc gttacctgct cttcatcgga cggatcgaac
     8761 ggcggaaggg cgtcgacgtc ctggccgccg ccctgcccaa ggcgctcgcc gcgcacccag
     8821 agctgcacgc tgtgtttctc ggccgggacg ccggggcggc gggtggcgac gcgggtcgcc
     8881 ggctcgacga tctactcgag gcattccccg gtcgtgtgca ccggcccgga gcgctgccgc
     8941 gtgagcaagc gctgcccgtg gttgcgcgtg cgtcgcttgt ggtgctgccg tcgctttggg
     9001 aggcgttcgg cttcgtcgcg gtggaggcga tggcacttgg caggcctgtg atcgcgagct
     9061 caggtagcgg cttcgaggag atcatcgaac acgaccgaag cggctggctc gtgccgcccg
     9121 gtgacgcgcc cgcgctcgcg gaggcgctgg tggagcgagc gggcgacgcg gtggagctcg
     9181 accggatctc gcgtggcgcg cgcgtgcggg cggacgattt cgaggcgtcg cggatcgcgc
     9241 cgatgctcga gcgcgtctat gagcgcgcca tcgagcgagc ggacagcccg ggccacttca
     9301 ccgacgcgat ctacagccgc ggctaccggc gattcttccg gccggaggac agcggcaacc
     9361 ccttccgcca gatatacgac gagaagctcg aggcggttgt cggacgcctc cgcgccatgc
     9421 cgtcgatgcg catcctcgac gtggggtgcg gccccgggcg ggcgctggcg cctctcgcgc
     9481 tgcgccacca gatgacgggc tgcgacgtgt cggccgaaat gctcgaagag gcacgacgac
     9541 gctgcccgtc acacgttgag ctcgtgcgcg cggacgcccg ctcactgccg ttcgccgacg
     9601 agagcttcga cgcagtgatc gcgctcgacc tgctgacgca tcttcctgac cttcgagcgg
     9661 gcgtccgcga actcgcgcga gtggcgcgca gcggcggtcc ggtgatcttc gacacgtcca
     9721 atgccgagcc gtggtgggtg ctcgcgtatc cgcgctacgt gaactggcgg ccacgccggc
     9781 tgatcgccac gatgcgtcga ggcggggtcc tgcccgagtg gagcgagatc gttcgccacc
     9841 accgagccga tgaggctcgc gccgcaatcg aggacgccgg gctggcgatc gaggacgtcg
     9901 gcaccttcgg accgcggtgg actcctaagt ggcacctctg gtacacgcgc aaggggttgc
     9961 cgtgagcgcg ctcccgcgcg caccgctgct cttctacctc gctcgggggg acgatccaca
    10021 acggcttgcg gtcggtccgg cacttgcggc ggcggccggg cgggccggct ggcagttcga
    10081 gtgctactac gccgaggcac gccgaggccg ccattacgga ggcggcgacc cctccggagc
    10141 gcgcgcgcag ctcgccgcgg gctcgctgtt cgcgggagga atgcacgccg agcgagcgtt
    10201 ccggatcgcg gtgggctacg aggtcgccgc gctcggcgat cctggctcgc ccctgtggcc
    10261 cgtgctcgac gaggccggcg gcgcggcgct cgcgcaaacg gaggatcccg tcgagttgta
    10321 cgtcgccgca ctcgccgcgc tggtccagga cgtgccggac atggtcctgg ttctggatgc
    10381 cgctccgcag ggaccccacc gcgtggtcac cgcgccgtac ctgtatccgg cgctgctcgc
    10441 gggcgagccc gcgtgggccc tcgaggcgtc ggcgggcgat gaccaacgga cggcactcga
    10501 gcggctcggg gtccggcact tcagaggcct cggggtggac ggcgagcgag cgctcgactt
    10561 cccgggatcg gtcgagccgg gcgacgtctt tcccgtggac gacgggtggg ccgcgttcac
    10621 ggtcgccgcc gcgcggacgc accgcgcctg gggccggggc gtgctgctgg gcgatcctgc
    10681 gctggtggcc gcgcagctgc cgcgcgcgcg ccggttgcgg ctgctgccgc tccatggaag
    10741 gcctcagacg gacgccatcg acgcggcgag cgacatcgtc agcgaggcgc gcgaaccggt
    10801 cttcgggcgc cagtgggacg accgtgattt cttcgcgctt gcctcggctg ggcacggcct
    10861 ccaggtcgtc gatccggggc ctccgttcga ctcggaggcc ggcatcgccg cgagggtgcc
    10921 ggaaccgccc gaggacgagg cggcgcccga gccgggcgac gacgagctct tgcgctgggc
    10981 gaacgagggg cgcgtgctcg tgacgctgtt gctctggacc ggcatggtgc gagagctcga
    11041 ctgcataccg cgcctgatcg acctcgcgat ggccaccgat cttcgcgcgg gcctggttct
    11101 gacggccgag agctacgagc agggcggagc gcacacgctt ggcccgctcg gcgtcccggt
    11161 cgcccgcggg ggtgtgctcg gccagatcga gccactgctc ggaagcaccg gtcgcggcgt
    11221 ggcggcagag gcctacctcg gcggcggacg gcttcggggg tacctcaccg aggcgctggc
    11281 cgcgatcgac gcgcgagcgc cacgagcgct gcgcccccgc ggttggtggc cgctgctcga
    11341 cgcgcgtctc gtgcgccgcc gcgagctcgc ggtgggtcgt cgcgggcggc gcccggtcgc
    11401 gcgcttcaac ccccgcgcgg aggcatcgcc ggtcggagcg ggtgcttctt ctgaatcaac
    11461 aaagccgcgg cgcgatctgc gcggcatcgc cgggagcgcg atcaggcgca ccggcctcga
    11521 ctcgttcctc gaggagcggc gtccgttcga cggctggcgc cccggtgtgc tcgacgaggg
    11581 cgtggcgtcg gcagtccacg gcgcgggcct cgagtacatg tggacgaaga cggccttcgg
    11641 caggccgcgg gtcgtcctcc aagatggcga cttcgtcgcg ctgcccttca cagccggctc
    11701 gtgggacgga tggtcgcctt tctacacggt cggatcccac cacggtctgc ttcgggcgga
    11761 gcgccggctt cgccgccacg gagagcccgg atggctcgcc acgacgatcg actcgcctct
    11821 cttcgccatg tccggcgagg tgctcgagca tggtgcggcc ttgcacgaga tcgcgcgcgt
    11881 tgccgcgcag ggtggctcga gcggcgagct cgtgaacgtg accccgaacg tggtcgcgcg
    11941 ctatgcgcgg ctgctcgccc caagggggta gacgcacgat gatcgatctg acctggcctc
    12001 gggcgcggcc ccccgccctg atcgcagtct ccggaatcga cgggtcggga aagacgacgc
    12061 tcatcgacgc ccttcgagag cggctcggcg aagacggcta caaggtcgag gtcgtgtgga
    12121 cgcggctcga atggaccacg ctgtgggagt cctcgagtcg gctcgagcgc atcaccgcgc
    12181 tcgtggactg ggtgcctgcg cgcaaggaga ccgcgccggc ggcgcccgag cccgccgagc
    12241 cgacttcggc gtggctcgag ccggcgcccg cccgcagcgc ggcggcacag ctcaggcgtc
    12301 gcagccgact gctgactcgc gcctgggccg gtgtcgtggc gacccttcac gctcaggcgc
    12361 agcgtaaggc cgtcgtgccc cacctacgcg agggcaaggt tgtcatctgc gaccgctaca
    12421 cgctcgacgc ggccgtggcc ctccaccgtc gctacgacag cgtccgttcg ctcggcctgc
    12481 aggtgcgcct gatgcagttc ctgagcccgc ggccgctgct cgcctggcac ctcgaccttc
    12541 ccgtcgccgt ggccgcgcaa cgaaaggagc acgagctcgg gatcgccgag cgacagcgcg
    12601 aaaacgtgct ctacaggcgg gagcgggagc gcctcggatg gagccggctc gacgccgcgc
    12661 gaccggtcga cgagctcgtg cagcaggtca cccgcgaagc cgaatcggcc ttggca
//
LOCUS       DASVXS010000061         9773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_998546, whole genome shotgun sequence.
ACCESSION   DASVXS010000061 DASVXS010000000
VERSION     DASVXS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998546"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /gene="murJ"
                     /locus_tag="VF752_03360"
     CDS             complement(<1..1524)
                     /gene="murJ"
                     /locus_tag="VF752_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX8854613.1"
                     /translation="MAAGETTTTTDPAGTGTVESPTQRGRRLAVNTAFFSAATGLSRI
                     AGLVREVVAAKYFGVTGPMSAFTIAFQVPNLVRALFADAALQGAFVPVFTELLETGKK
                     KEAFRVASTLFLMILMILGGVTLLFELVAPFVMKIVAPGFADNPVLKDLTVTMGRILF
                     PTVLLLALSGVIVGMLNSFEHFAVPALAPLVWNLVIIGGLVGLTPLWSGPQEIYGYAW
                     GVLIATFVQLLLPVPWLRGRGGRFTLTLDWRNPHVARVLKLMLPVTIALGLINFALLI
                     NSFFGTLVSNQAPAAIDKAFRIYQLPQGVFSIAIATVAFPTLSRFAARKNYDDLRRTM
                     ANAMRQIFMLLIPSAVIMAVLATPITRLIYQRGEFGPGATHLVAQAMFWWSFSLPFQG
                     VSLLFSRTFFSLQRPWITTALAGGNLVVNAGLAAALYKPFGVGGVVLGTVIGTIAMAV
                     AQGYMLRGILGGIQGARTLSVISRMLVAAAVLAAVSYVVWWGLDKAFRIYQLPQGVFS
                     "
     gene            1578..1838
                     /gene="rpsT"
                     /locus_tag="VF752_03365"
     CDS             1578..1838
                     /gene="rpsT"
                     /locus_tag="VF752_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX8854614.1"
                     /translation="MANIASQEKRIHRSERERLENRRYTSTIKTYFRRLETAVEAGDA
                     ETAESEHRTLVSTIDRAVKRGALHANNGARKKSRAAALRSRL"
     gene            complement(1946..2383)
                     /locus_tag="VF752_03370"
     CDS             complement(1946..2383)
                     /locus_tag="VF752_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX8854615.1"
                     /translation="MARVSTYDEELAADLRAGGQRVTSQRLLINRTLREMNRHVTAEE
                     VLSEVSERLPGISLPTIYSTLELFERLGVVRRVSAGGGAVLYDPRREEHSHLICERCG
                     RVEDLDATLDASTALSAAKRSGFKPARAELVVHGLCSACAAQQ"
     gene            2449..2955
                     /locus_tag="VF752_03375"
     CDS             2449..2955
                     /locus_tag="VF752_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854616.1"
                     /translation="MAALTAALERWRTADDRERLLRVVQPGLIGLIDGTLSTLAPIFA
                     LAYFAGSKAALVAGLAAALGAAISMGMSEGLSDDGELTGRGSSLVRGMVTGLATFVGG
                     TFHALPFLIQDVHTALAVAYVVVAIELAVIAWVRKRFLKVGFTQSLVQVIIGGIVISA
                     VGITVGAA"
     gene            complement(2936..3343)
                     /locus_tag="VF752_03380"
     CDS             complement(2936..3343)
                     /locus_tag="VF752_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854617.1"
                     /translation="MSERNVRFIRAAFDAFSRGDFEAVLQVTDPDIEITQPRELPDYR
                     TYHGREGVLEAFRDWPAQWDQFDVDLVDVIDLDDEHVVSVTRQHLRARELEFDQDVIN
                     LHTFRGEKAIGWHMFFTVEEAMEAARAAKPRPR"
     gene            complement(3346..4308)
                     /gene="holA"
                     /locus_tag="VF752_03385"
     CDS             complement(3346..4308)
                     /gene="holA"
                     /locus_tag="VF752_03385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX8854618.1"
                     /translation="MADLKSVYLICGEDDAKIDAWRARLRKRAEAENGPGALEIFDGR
                     ENAPDDVAAAMSMLTFGTGTRYLLVDGAEAWKAGELEPLERQLADPPPDTVLVLLARG
                     KGAPKRLEKAVEKAGGEQRSYSAPKPWELPSWVVERAREEGLRLDKDAAKVLIASVGQ
                     SQQRLSREVERLAIAAHPAGHLSADEVQELSAGDAGRGAYDLADAVVAGDLRATFAIA
                     ERLVQRDERPSRLMYTVVRRLREVHRAAALLDAGMPEQKVGESLGGPPWAAKKVVAAA
                     RGADPEALERALCALADLEVELRGGGVGFDEDTAFSLALAQAAG"
     gene            4483..4992
                     /locus_tag="VF752_03390"
     CDS             4483..4992
                     /locus_tag="VF752_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8854619.1"
                     /translation="MLSAGPTPHTPLDEWSFSLQGAVEPGRSWTWDEFQALPSETVTK
                     DIHCVTKWSKLDTVWEGVSVDVLLDGAQLDGGFTTVFCDGGYTTNLAIEDVTGGKAWV
                     AYKFGGEPLAPEHGGPARLLVPHLYFWKSAKWVRGIQVDRRDEPGFWESFGYHNRGDP
                     WLEQRYAGD"
     gene            4967..5737
                     /locus_tag="VF752_03395"
     CDS             4967..5737
                     /locus_tag="VF752_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX8854620.1"
                     /translation="MSSATRATEFTRPVRWQLATVADAIAEAEGARTLVLDLPNWPGH
                     LAGQHVDVRLTAEDGYQAERSYSIGSPPEDARLALTIELLEDGEVSPYLVDEVREGDM
                     FEVRGPIGGWFTWQASDGGPLLLVAGGSGIVPLMAMIRHRANVHSEVPTRLLVSARSL
                     EALLYRAELDSLASAGDGLEVVTTLTREAPDDWTGHTGRVDGNLLREVAFPPDELPRV
                     YVCGPTPFVENVAESLVRLGHDPALVKTERFGPTGGTK"
     gene            5734..6006
                     /locus_tag="VF752_03400"
     CDS             5734..6006
                     /locus_tag="VF752_03400"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX8854621.1"
                     /translation="MIETQRHLDGNAVAGLLAQVFNADMTSAMCTCGTCGNTSPLAMA
                     MVYPGGPAHVLRCAVCDSVVMKMAELPGRIVLEASGVMRMQFSDAS"
     gene            complement(5990..8332)
                     /locus_tag="VF752_03405"
     CDS             complement(5990..8332)
                     /locus_tag="VF752_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX8854622.1"
                     /translation="MPDAALQKALFELRSRPHLCLVVAFSAGLLACRQAIVLALLALV
                     GAAALSAAGDRRLAAMAAAALVAGGLAGSARLHAIDAPAAHLRSGQRLAATVELVEAP
                     RPGRFGGWSAEARVITGAARGARLLARSDVNTRLRRGARAAVAGLVIRPRRSPGDSFD
                     YGAYLRRRGIAYALELESLRPLPGRPGGIDTLRRRAEHALAQGVSGERAALAAGMVLG
                     EDERISPELKDDFRRAGLGHLLAVSGQNVVLLGALALPVLAAAGLGIRARAVVLLCLI
                     AVYVPLAGGEPSLQRAGAMGAAGLLALLAGRPASRWYALALAAAATLLVNPRVGGDPG
                     WQLSFAAVTGLLLLGSPLRRVFAPLPHALAEGIGATVAATLATLPLAAFHFGSLPAWS
                     LVANLAALPAVAPAMWLGMVQVALAPFAPDAVQAAIGQATTLALGWIELVARWFSGAP
                     GARLELGLASAPALAGVYAALAAACVGAWRLAHRAEPRLRAGAAEWRRLPRRERRLAA
                     VAAALALVGVVYVATGPARPPRHLTVSFLDIGQGDATLIQDPHGTAVLFDGGPPEARV
                     ALKLRRLGVRRLSLMVSTHQSRDHQGGLHEVLQRIPTGLLLENGDGTSDPDYRRLIAE
                     ADRRGIRHVAARAGQVLHAGALTIRLLNPKPLPPGAPRPSDPNPRGVAAIVSSGSFDL
                     WLSADAESDAILPLPLRRVEAMKVSHHGSADPGLPQVLRRLRPQIAAIEVGAHNLYGH
                     PRPSTLAALNVAVPHVYRTDRDGTVQLTVEGGKIQVRTHR"
     gene            complement(8811..9410)
                     /locus_tag="VF752_03410"
     CDS             complement(8811..9410)
                     /locus_tag="VF752_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX8854623.1"
                     /translation="MDESDRLRLVVWGAIALLVLFAGWRLLGDRAGASEASVKFDGPG
                     AAAPSRARPAREPPARVYVAVVGSVRRPGLYRVPTGARVAAAVARAGGLGRGADLTGV
                     NLAARVHDGQQIVVPKLGAAAAGAVAGGPTSATTGPISLSTATVEQLDGLEGIGPALA
                     QRIVAYRQSHGGFRSIEELGQVEGIGDKRLAALRSAVTP"
     gene            9476..>9773
                     /locus_tag="VF752_03415"
     CDS             9476..>9773
                     /locus_tag="VF752_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8854624.1"
                     /translation="MKVHRYNAHAHWRGSTGAGWEHYSRAHEVSAPPAEPALTVTTGE
                     AKGDPRQLNPEQLLLIAASTCQLLWFLHLASKARIDVVEYRDEAEAEMHQDDKPV"
BASE COUNT         1536 a         3480 c         3399 g         1358 t
ORIGIN      
        1 cgagaacacg ccctgcggca gctggtagat ccggaacgcc ttgtcgagtc cccaccacac
       61 gacgtagctc accgcggcca gcacggcggc cgccacgagc atgcgcgaga tgaccgagag
      121 cgtgcgcgct ccctggatcc cgccgaggat cccgcgcagc atgtagccct gggcaaccgc
      181 catcgcgatc gtgccgatca cggtgccgag cacgacgccg ccgaccccga acggcttgta
      241 gagcgccgcg gcgagaccgg cgttcaccac caggttgccg ccggccagcg cggtcgtgat
      301 ccacgggcgc tggaggctga agaaggtgcg cgagaagagc aggctcacgc cttggaacgg
      361 cagcgagaac gaccaccaga acatcgcctg cgcgaccagg tgcgtggcgc ccgggccgaa
      421 ttcgccgcgc tgatagatga ggcgcgttat cggcgtcgcg agcacggcca tgatcaccgc
      481 cgacgggatc agcagcatga agatctgccg catcgcgttc gccatcgtgc ggcgcaggtc
      541 gtcgtagttc ttacgcgccg cgaagcggga gagcgtcggg aacgccacgg tcgcgatcgc
      601 gatcgagaac acgccctgcg gcagctggta gatccggaac gccttgtcga tcgcggccgg
      661 cgcctggttg ctcacgagcg tgccgaagaa gctgttgatc aggagcgcga agttgatgag
      721 accgagcgcg atcgtgaccg gcagcatcag cttcaggacc cgcgccacgt gcgggttgcg
      781 ccagtcgagc gtgagcgtga acctccctcc gcgcccgcgc agccagggga ccggcaggag
      841 cagctgcacg aaggtcgcga tcaggacgcc ccaggcatag ccgtaaatct cctgcgggcc
      901 ggaccagagc ggcgtgagcc ccacgagccc gccaatgatc acgaggttcc acaccagcgg
      961 cgcgagcgcc ggcaccgcga agtgctcgaa cgagttgagc atccccacga tcacgcccga
     1021 cagcgcgagg agcaggacgg tcgggaagag gatccgcccc atcgtcaccg tgaggtcctt
     1081 gagcaccggg ttgtccgcga agcccggcgc cacgatcttc atcacgaacg gcgcaaccag
     1141 ctcgaacagc agcgtcacgc cgccgaggat catcaggatc atcaggaaga gcgtcgaggc
     1201 gacccggaag gcctccttct tcttgccggt ctcgagcagc tcggtgaaga ccggcacgaa
     1261 cgccccctgg agcgccgcgt cggcgaacag cgcgcgcacg aggttcggca cctggaaggc
     1321 gatggtgaac gccgacatcg ggcccgtgac gccgaagtac tttgcggcga ccacctcgcg
     1381 caccagccca gcgatccgtg acaggccggt ggccgcggag aagaacgcgg tattgaccgc
     1441 cagccggcgg cctctctgag tgggcgactc gaccgttccg gtgcccgccg ggtcggtcgt
     1501 ggtcgtggtc tcaccggccg ccacgaggcc gctatagtag gccgctgccg cgacccgctc
     1561 gcggctgctc tgtgtctatg gcgaacatcg cttcacaaga aaagcgcatt caccggtctg
     1621 agcgcgagcg cctggagaac cggcgctaca cctcgacgat caagacctac ttccgccgcc
     1681 tcgagacggc ggtcgaggcg ggcgacgcgg agaccgccga gagcgagcac cgcacgctcg
     1741 tgtcgaccat cgaccgggcc gtgaagcgcg gcgcgcttca cgcgaacaac ggcgcacgta
     1801 agaagtcccg cgccgctgct ctccgcagcc gcctctaaag gcgactgcaa accttcgtct
     1861 ggcggcgtca tcggtcgctc gtgtgcagag cacacgtcgc tccctgcttc cttgccagac
     1921 tcggttttcg ccgcacaact cggacttact gctgtgctgc gcacgcggag cagagcccgt
     1981 gaaccactag ctcggcgcgg gcgggcttga acccgcttcg cttcgcggcg ctcagagcgg
     2041 ttgacgcgtc gagcgtcgcg tccaggtcct cgacccggcc acagcgctcg cagatgaggt
     2101 ggctgtgctc ctcgcggcgc gggtcgtaca gcaccgcccc gccacccgcg ctcactcgcc
     2161 gcacgacgcc gagccgctcg aacagctcga gcgtggagta gatcgtcgga agcgagatgc
     2221 ccgggaggcg ctcgctcacc tcggagagga cctcctcggc ggtgacgtgg cgattcatct
     2281 cgcgcagcgt gcggttgatc agcaggcgct gagaggtcac ccgctggccg ccggctcgga
     2341 ggtcggccgc cagctcttcg tcgtatgtgc tcacccgtgc catgcgcttc aagtgtagtt
     2401 cctattaggg agaccttatt ttaactcatt tgctattgtg atctaccgat ggcagccctc
     2461 accgcagcac tggagcgttg gcgcacggcg gacgaccgtg aacgcctcct gcgcgtggtc
     2521 cagccgggcc tgatcggcct gatcgacggg actctctcca cgcttgcgcc gatcttcgcg
     2581 ctcgcctact tcgccggctc gaaggccgcc ctcgtggcgg gcctcgcagc cgcgctgggc
     2641 gccgcgatca gcatgggcat gtccgagggc ctgtccgacg acggcgagct caccggacgc
     2701 ggatcctcgc tcgtgcgagg catggtcacg gggctcgcca ccttcgtggg cggcaccttc
     2761 cacgccctgc cgttcctcat ccaggacgtg cacaccgcgc tcgcggtggc ctacgtggtg
     2821 gtggcgatcg agctggcagt gatcgcctgg gtgcgcaagc gcttcctgaa ggtcggcttc
     2881 acccagtcgc tcgttcaggt gatcatcggc ggcatcgtga tctccgccgt cggcatcacc
     2941 gtgggcgcgg cttagccgcc cgcgcggctt ccatcgcctc ctcgacggtg aagaacatgt
     3001 gccagccgat ggccttctca ccgcggaagg tgtgcaggtt gatcacgtcc tgatcgaact
     3061 cgagctcccg agcacgcagg tgctggcggg tgaccgacac gacatgctcg tcatccaagt
     3121 cgatcacgtc gacgaggtcg acgtcgaact gatcccactg cgccggccag tcgcggaacg
     3181 cctcgagcac gccctcgcgg ccgtggtagg tgcggtagtc cggtagctca cgcggctgcg
     3241 tgatctcgat gtccgggtcg gtgacctgga ggaccgcctc gaagtcgccg cgactgaacg
     3301 catcgaacgc cgcgcggatg aagcgcacgt tccgctcgga catgctcagc ccgcggcttg
     3361 cgcgagtgcg agggagaacg cggtgtcctc gtcgaagccc acgccgccgc cgcgcagctc
     3421 gacctcgagg tcggcaagcg cgcacagggc gcgctcgagc gcctcggggt ccgctccgcg
     3481 agccgcggcg accaccttct tcgcagccca gggcggaccg ccgagcgact cccccacctt
     3541 ctgctccggc atcccggcgt cgagcagcgc ggccgcgcgg tgcacttcgc gcaggcgccg
     3601 cacgacggtg tacatgaggc ggctcgggcg ctcgtcgcgc tgcaccaggc gctcggcgat
     3661 cgcaaacgtg gcccgcaggt cgcccgcgac caccgcgtcg gcaaggtcgt aggcgcctcg
     3721 cccagcgtcg cccgcggaga gctcctggac ctcgtcggcg ctgaggtgcc cggccgggtg
     3781 ggcggcgatc gcgagccgct caacctcacg ggagagccgc tgctggctct gaccaaccga
     3841 cgcgatcagc accttcgccg cgtccttgtc gagacgtagc ccctcctcgc gggctcgctc
     3901 gaccacccag gacggcagct cccacggctt cggcgcgctg taggagcgtt gctcgccgcc
     3961 cgccttctcg accgccttct cgaggcgctt cggcgcaccc ttgccgcgag ccagcaacac
     4021 caggacggtg tcgggcggcg ggtcggccag ctggcgctcg agcggttcga gctcacccgc
     4081 cttccacgcc tcggccccgt ccaccagcag gtagcgggtg ccggtgccga aggtgagcat
     4141 cgacatcgcg gcagccacgt cgtccggcgc gttctcgcgg ccgtcaaaga tctcgagcgc
     4201 gcccggcccg ttctccgcct ccgcccgctt gcggagccgc gcccgccagg cgtcgatctt
     4261 ggcgtcgtcc tcgccgcaga tcagatagac ggacttcaga tcggccatcg ggcgagtgta
     4321 ttgacgccgc ccggacgggt tggcttgatc cagggcgtac gctgttcgtg ttctcggaga
     4381 aagtgggcct gcatgtcccc gatctctcgc ggcttccgcg gccgcccccg acaagaaagc
     4441 gacaggcttc cgccaggcca gtacctggtc gaggatttcc cggtgctctc cgccgggccg
     4501 accccgcaca cgccgctcga cgaatggagc ttctcgctgc agggagcggt cgaaccggga
     4561 cgcagctgga cttgggacga gttccaggcc ctcccgagcg agacggtgac gaaggacatc
     4621 cactgcgtga ccaagtggtc gaagctcgac accgtctggg agggcgtctc tgtggacgtg
     4681 ctgctcgacg gcgcgcagct cgacggcggc ttcaccacgg tgttctgcga cggcggctac
     4741 accaccaacc tcgcgatcga agacgtgacc ggcggcaagg cgtgggtcgc ctacaagttc
     4801 ggcggcgagc cgctcgcgcc ggaacacggc ggtccagcgc ggctgctggt gccacacctg
     4861 tacttctgga agagcgccaa atgggtgcgc ggcatccagg tggaccgccg cgacgagccg
     4921 ggcttctggg agagctttgg ctaccacaac cgcggagacc cgtggcttga gcagcgctac
     4981 gcgggcgact gagttcacga ggccggtccg ctggcagctc gcgacggtcg ctgacgcgat
     5041 cgccgaggcg gagggcgcgc gcacgctggt cctcgatctg cccaactggc ccggccacct
     5101 ggccggccag cacgtggacg tccgcctcac cgccgaggac ggctatcagg ccgagcgcag
     5161 ctattcgatc ggctcgccgc ccgaggacgc gaggctggcc ctcacgatcg aactcctcga
     5221 agacggcgag gtgtcgccct acctcgtgga cgaggtgcgg gagggcgaca tgttcgaggt
     5281 gcgcggcccc atcggcggct ggttcacctg gcaggcgagt gacggcgggc cgctgctgct
     5341 agtggcgggt ggctcgggca tcgtgccgct gatggcgatg atccgccacc gcgcaaacgt
     5401 gcacagcgag gttccgacgc gcctgctcgt gtccgcgcgc agcctcgagg cgctgctcta
     5461 ccgggccgag ctcgactcgc tcgcttcggc gggcgacggc ctggaagtgg tgacgacgct
     5521 cacgcgcgag gcgcccgacg attggaccgg ccacaccggc cgcgtggacg gcaacctgct
     5581 gcgcgaggtc gcatttcctc cggacgagct gccgcgcgtg tacgtgtgcg ggccgacgcc
     5641 cttcgtggaa aacgtggccg agagcctggt ccggctgggc cacgacccgg cccttgtgaa
     5701 gacggaacgc ttcggtccca cgggaggcac aaaatgatcg agacccagag acacctcgac
     5761 ggtaacgccg tagccggcct gctcgcgcag gtgttcaacg ccgacatgac gagcgcgatg
     5821 tgcacctgcg gcacctgcgg caacacgagc ccgcttgcga tggccatggt ctacccgggc
     5881 gggcccgcac acgtcctacg ctgcgcggtg tgcgactcgg tggtgatgaa gatggccgag
     5941 ctgccgggcc gcatcgtgct cgaagcgagc ggcgtgatgc gcatgcagtt cagcgatgcg
     6001 tcctgacctg gatcttcccg ccctcgaccg tcagctgaac cgtgccgtcc cgatcggtgc
     6061 ggtagacgtg cggcacggca acgttcagag ccgcgagcgt ggacggtcgc gggtggccat
     6121 agaggttgtg cgcgccgacc tcgatcgcgg cgatctgcgg gcgtaagcgc ctcagcacct
     6181 gaggcagccc cggatcggcg ctgccgtggt gcgacacctt catcgcctcg acacgtcgga
     6241 gcggcagcgg caggatcgcg tcgctctcgg cgtccgctga gagccagagg tcgaagctcc
     6301 ccgagctcac gatcgccgca acgccgcgcg ggttcgggtc cgagggccgc ggcgcgcccg
     6361 gcggaagcgg cttcgggttg aggaggcgaa tcgtgagcgc acccgcgtga agcacctgcc
     6421 cggcccgcgc ggccacgtgc cggatgccgc gccggtcggc ttcagcgatc aggcggcggt
     6481 agtcggggtc gctcgtgccg tcgccgttct cgagcagcag gccggtcggg atccgctgca
     6541 gcacctcgtg cagaccgccc tggtggtcgc gcgactggtg cgtcgacacc atcaggctca
     6601 ggcgccgcac gcccaggcgc cgcagtttga gggcgacgcg cgcctcgggc gggccgccgt
     6661 cgaacaggac cgccgttcca tgcgggtcct gaatcagcgt ggcgtcaccc tggccgatgt
     6721 cgaggaagga gacggtcagg tggcgcggcg ggcgcgccgg ccctgtcgcc acgtacacga
     6781 cacctacaag cgcgagcgcg gcggcgacgg cggccagccg gcgctcccgg cgcgggagcc
     6841 gccgccactc ggcggccccg gcgcgcaatc gaggctcggc gcggtgcgcg agtcgccacg
     6901 cgccgacgca ggcggccgca agcgcggcat agaccccggc cagggccggc gccgacgcga
     6961 ggccgagctc cagccgggcc cccggcgcac cggagaacca gcgcgcgacg agctcgatcc
     7021 atccgagcgc gagcgtggtc gcctgcccga tcgccgcctg gactgcgtcg ggcgcgaaag
     7081 gcgcgagcgc gacctgcacc atgccaagcc acatcgccgg cgccaccgcg gggagcgccg
     7141 ccaggttcgc gacgagcgac caggcgggca gcgagccgaa gtggaacgca gcgagaggca
     7201 gcgttgcgag cgtcgcggcg acggtcgcgc cgatgccctc ggcgagcgcg tgcggaaggg
     7261 gcgcgaagac gcggcgcagc ggcgagccga gaaggagcag tccggtcacg gcggcgaacg
     7321 acagctgcca cccgggatcg ccgcctaccc gcgggttcac gagcagcgtc gcggcggcgg
     7381 caagcgcgag cgcgtaccag cgagaggccg gccgccccgc gagcagcgcc agcaagcctg
     7441 cggcgcccat cgcgccggcg cgctggagcg acggctcgcc tcctgccagc ggcacgtaaa
     7501 cggcgatgag gcagagcagc acgaccgcac gggcgcggat cccaagaccc gccgccgcga
     7561 gcaccggcag cgccagcgcg ccgagcaaca ccacgttctg accgctcacg gcgagcaggt
     7621 gaccgaggcc cgcgcggcgg aagtcgtcct tcagctccgg cgagatgcgc tcgtcctctc
     7681 cgagcaccat cccggcggca agcgcggcgc gctcgccgct cacgccctgc gcgagcgcgt
     7741 gctcggcgcg ccggcgcagc gtgtcgatgc cgccggggcg gccggggagc ggccgaagcg
     7801 actcgagctc gagcgcgtag gcgatgccac ggcggcgcag gtacgcgccg tagtcgaacg
     7861 agtcgcccgg cgagcggcgc ggcctgatca caagccccgc gaccgcggcg cgcgcgcccc
     7921 gtctaagccg cgtgtttacg tccgagcggg cgagcagccg tgcgcctcgc gcggcacccg
     7981 tgatgaccct cgcctcggcg ctccagccgc cgaagcggcc gggccgaggc gcctcgacga
     8041 gctctaccgt cgcggcgagg cgctgacccg agcggagatg ggccgccggt gcgtcgatcg
     8101 cgtgtagccg agccgagcca gcgagcccgc ccgccacgag ggctgcggcg gccatcgcgg
     8161 cgagccgcct gtcaccagcg gccgagagcg cagccgctcc gaccagcgcg aggagtgcga
     8221 ggacgattgc ctgccggcat gcgagcagcc ccgcactgaa ggcgacgacg aggcacaggt
     8281 gcgggcgcga gcggagctcg aagagcgcct tctgcagcgc ggcgtccggc atccgcgcga
     8341 cgctacggcg gccgggctca cggcactgga acgagcgtta caactccggc gcggcgatcc
     8401 gccgcgcggg ctgagcgact ccgagtgtcg ctacgcggct tgcttgtgcg caggcttggg
     8461 tgcgtggcac gccgaagcgt ggattgcacg cggcttgcca tggcggtaac tcgccaggat
     8521 tcggtcgccc ggcttgagct gggcgtggca gatcccgcag caaagctctg caaagcggct
     8581 gtcccgctca accagaagca ttctgcgcct ccctgtggtg aatacgcctc actgccatat
     8641 cgcagacctt aggactctgt tcggcggaaa gcgggtgctt ccttgatgtc cccagaatgc
     8701 ggtacacgtt ctatgcccta tcacaagaat gacgaaaccg tcatgaaatt ggcattaatg
     8761 tatgcgcaac agaccatttc ccttcgcaaa atggtcggta atcgttcctc tcacggcgtg
     8821 acggcgcttc gcagggcggc caaccgcttg tcgccgatcc cctccacctg accgagctcc
     8881 tcgatcgagc gaaagccgcc gtgcgactgg cggtaggcca caattcgctg agcgagcgcc
     8941 ggcccgatgc cctccaggcc gtcgagctgc tcgaccgtcg cggtcgagag gcttatcggt
     9001 ccggtggttg cggacgtcgg tccgccggcc acggctcccg cagccgccgc tcccagcttc
     9061 ggcaccacga tctgctgccc gtcgtgcacg cgcgcggcga ggttcacgcc cgtgaggtcg
     9121 gctccacggc cgaggccacc ggcgcgagct acggcggccg ccacacgcgc gccggtggga
     9181 actcggtaca ggcccggcct tcgaaccgag ccgacgaccg cgacatagac cctcgccggc
     9241 ggctcccgcg ccggccgtgc gcggctcggc gccgccgcgc cgggcccgtc gaacttcacc
     9301 gacgcctccg acgcgccggc gcggtccccg agcagccgcc agccggcaaa cagcacgagc
     9361 agcgcgatcg caccccacac gaccagcctc agcctgtcgc tctcgtccat ggcgggaacc
     9421 tacgaatgcc cgtgtgacgg gtgggttacg tgtgcgcccg cttaggatcg ggcgcgtgaa
     9481 ggtccaccgc tacaacgctc acgcgcattg gcgcggctcg acgggcgcgg gctgggagca
     9541 ctactcccgc gctcacgagg tgagcgcacc gcccgctgag ccggcgctga ccgtgacgac
     9601 cggcgaggcg aagggcgacc cgcgccagct gaacccggag caactgctct tgatagcggc
     9661 gtcgacctgc cagctcctgt ggttcctgca cctcgcgtcc aaggcgcgca tcgacgtggt
     9721 cgagtaccga gacgaggcgg aggccgagat gcaccaggac gacaagccgg tcc
//
LOCUS       DASVXS010000062         9129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_156409, whole genome shotgun sequence.
ACCESSION   DASVXS010000062 DASVXS010000000
VERSION     DASVXS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156409"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..298
                     /locus_tag="VF752_03420"
     CDS             152..298
                     /locus_tag="VF752_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854625.1"
                     /translation="MSKNGRNVHVEPWERQVGEPAERWEAFSVYRDQLVNTCDPEPVL
                     DARI"
     gene            complement(554..1861)
                     /locus_tag="VF752_03425"
     CDS             complement(554..1861)
                     /locus_tag="VF752_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX8854626.1"
                     /translation="MPRSVKGLWVAGFAGAATLPLAVALVGSPPSGRGFVIELGSGFG
                     IVALSVLALQLLLPARVRPVTAWLGAEVAIRLHRRLADLLVAAIVAHVALVVVGTPAN
                     LALFDLLGAPWRARAAFASSAALAALVASSVLRRRLRLSYARWRGLHLLLGLSALALA
                     ILHALGVGRYLSSPAGLTVAALAALAVLAVVQLRLLRPRRLAGRPYLIERLVPERGGA
                     TTLALRAEGHRGRAFRPGQFAWLKLADAPHALAEHPFSYASSASRPERPMFTIKAYGG
                     FSRRIAQLAPGTRVMVDGPHGSYRPRPGAERLVFIAGGIGITPIVSLLRSAAEAGDRR
                     PMLLLYGSLRWEEIIFREELERLERRLALRVVHVLTEPAVGWAGETGFIDLELLQRHL
                     PCDPHRADIFVCGPPAMLAPVLQGLDRLGVAREQVHAEQFATV"
     gene            2594..5530
                     /locus_tag="VF752_03430"
     CDS             2594..5530
                     /locus_tag="VF752_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8854627.1"
                     /translation="MTTADSQRAGSLVGRARELAELEQTLDRVESGTHWAIEIVGEPG
                     IGKSRLIAELGRIAEARGYLVLDGRAAEFESDIPFGVIVDALNDYVGSLGPAVLRGLE
                     AGVVQELALILPSLSTLSGEQVGPRAQTERYRVHYAIRALLELLAKRQPVLLAIDDVH
                     WADAASFEVMAHLLRRFRGPMLAAFAFRYAPARLVGALEATARAGFGSRLELAPLSAA
                     EAATLLDPDLDGATRDALYLESGGNPFYLEQLARGPTGRSPAASGRGERLGEAWEPPP
                     GVVAAIDQELDRLSAGQRRTLDAAAVAGDSFDPLLVSAIAEQDEPTALAALDGLIEVG
                     LVRPTAVPRSFRFRHPIVRRAVYDALPGGWRVGAHARAAAGLASIHASGSELAHHVAR
                     SASIGDEHAISLLIDAARATAARAPLTAGQWLLAAARLVPSGDYERRVRLLGEAGASL
                     TSGGGFDDALTSLDEALSLAHPDSTGVRAGLIANRAEARRRGGRPFDSRGELERALES
                     PAGPEGPTAIAARLELAMDRYWYADFAAVHEHAAQALVFAREDRDPLLICLSAALDSL
                     ASSSRNLADDAFAQFRDAQAAYAALPDEQLAERVYLCHYMSEAALRLERSDDALAHHR
                     RGSEVARMTGQDATSHSWPGLAVYALLLKGELNEAARVAAGAINTADLARDDWKMIWV
                     LAADALASLSTGQNERALASAREMITRAERSHPGTSLNGLARVQLAAALAAAGDPAGA
                     HAELVGLDSEQSRWLLDLNCGQGWEVLIGARLAAGDVAAAGDAAATAESRVNSTRLQQ
                     RTAALRSARASVMLASGDAHAAAEAAEAAVQIAERTGNPVLTGRCLSQAGATLAAAGQ
                     RERGARQLERAERTLSACGAVREADAAARELRRLGRRVPRRARPNRGVGLAALSPRER
                     EVAEEVAAGKTNRDVAATLFLSEKTIESHLVRIYSKLDVHSRSALTAIVARQRGAADR
                     AAGRHS"
     gene            5863..6246
                     /locus_tag="VF752_03435"
     CDS             5863..6246
                     /locus_tag="VF752_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854628.1"
                     /translation="MSGASRASFESVALQLAPAERPATKSVGPSEQGLHVCPGCDSKL
                     VYPTSWEECPDNRWYLTLRCPECEFTSLGEFEQSAVDELELELERGDADLAADLSLFI
                     QANMVDFVNRFVRAIGADAIQPMDF"
     gene            6332..6457
                     /locus_tag="VF752_03440"
     CDS             6332..6457
                     /locus_tag="VF752_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854629.1"
                     /translation="MHQMRHIKSHTPAGMTAPTYRINRPRRHSAIRRFAGYGIVR"
     gene            6863..7027
                     /gene="rpmG"
                     /locus_tag="VF752_03445"
     CDS             6863..7027
                     /gene="rpmG"
                     /locus_tag="VF752_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX8854630.1"
                     /translation="MARGDVRIGVTLACEECKRRNYQTEKSKRNDPNRIELRKYCPWC
                     GKHTPHRETR"
     gene            7065..7529
                     /gene="secE"
                     /locus_tag="VF752_03450"
     CDS             7065..7529
                     /gene="secE"
                     /locus_tag="VF752_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX8854631.1"
                     /translation="MARNRQRAKQRQAERRAARIAQAGADTAPPLPENPDPELVEEAA
                     HLAAGAPPEDEGRSDTVLEEPSFEEEEEILGESEAHGRERALERDGRSHRRHGRVIAF
                     LIAVWAELKRVQWPDRQTLTVLTGVVLLFVLIMGGYLGLLDAAFSRLIQAIL"
     gene            7554..8075
                     /gene="nusG"
                     /locus_tag="VF752_03455"
     CDS             7554..8075
                     /gene="nusG"
                     /locus_tag="VF752_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX8854632.1"
                     /translation="MFRWYAVNTYSGHEKKVKQNLEHRVVSLNQGRNVRQIVIPTEQV
                     SEVKDGQKVQTERRTMPGYVLVNMDLNEDSWTLVKGTPGVTGFVGPQNKPVPLSQGEV
                     DRLLNREVAERPRTRAQFSIGESVKVVSGPLSDFSGEISEINEDGAKLKVLVSIFGRE
                     TPVEVGFDQVKKI"
     gene            8078..8500
                     /gene="rplK"
                     /locus_tag="VF752_03460"
     CDS             8078..8500
                     /gene="rplK"
                     /locus_tag="VF752_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX8854633.1"
                     /translation="MAKKVLTLIKLQVPGGAANPAPPVGPALGQHGVNIMEFCKAFNA
                     QTQDQNGRIIPVEITVYEDRSFTFITKTPPAAVLIKEAAGLDKGSAEPNRTKVARITP
                     DQVRQIAETKLQDLNARDLDAASKIIEGTARSMGVEVG"
     gene            8500..>9129
                     /gene="rplA"
                     /locus_tag="VF752_03465"
     CDS             8500..>9129
                     /gene="rplA"
                     /locus_tag="VF752_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX8854634.1"
                     /translation="MPKHGKKYREAYENVDRERDYEPNEAVSLVKSLQRAAFPETVEV
                     HIRTGLNVRHADEQLRGTITLPHGGGRDVTIAVFAKGDKAREAQDAGADVVGDEDLAK
                     RVEEGFTDFDVAIATPDMMPVVGRLGRILGPQGKMPNPKVGTVTNDVAQAVEESKAGK
                     VEYRTDRSAIVHLAIGKTDFEERALLENYAALIDEIVRAKPAAAKGRYLR"
BASE COUNT         1529 a         3133 c         3180 g         1287 t
ORIGIN      
        1 gttcgagtcc gacagtcggc tctctcagaa aggcccgcaa aggcgggtct tttccgtgcc
       61 gggaccgccg ccgccgaggc ccggagacgg ctcctgggca cactattggg caacacgagc
      121 ctccgccgcc gcgctcccta gggtcgtccc cgtgagcaag aacggccgca acgtccacgt
      181 cgagccgtgg gagcggcagg tcggtgagcc cgccgagcgc tgggaggcgt tcagcgtcta
      241 ccgggaccag ctcgtcaata cctgtgaccc cgagcctgtg ctcgacgcgc ggatctgatg
      301 gaccgcaacg tcgaaggcag aggttccatt ggtgaccggc caggcccgtc ggcgctgcga
      361 cgcgtcgcac gatccatgca tacggtcagg agaacggcgt gtgcgccggt ctcgagcgga
      421 gtcgcgcggg tgcgatcgcg gtcatctcgt caggtgcttt gcagggcggc gaaggcgact
      481 gtggcgatca ggcaccagag ctcgatcgcg gtggccatcc agtgggtctc atcgcggcgc
      541 atacgcatct cctttagacg gtcgcgaact gctcggcgtg tacctgttcg cgagcgacgc
      601 ccagccggtc gagcccctga agaacggggg cgagcattgc tggcggcccg cagacgaaga
      661 tgtcggcgcg gtgtgggtcg caggggaggt ggcgctggag cagctccagg tcgatgaacc
      721 cggtctcgcc cgcccagccc accgccggct cggtgaggac atggacgacg cgcagcgcga
      781 gccggcgctc aaggcgctcg agctcctcgc ggaagatgat ctcttcccag cgcaggctgc
      841 cgtagaggag cagcatcggc cggcgatcgc cggcttcggc ggcactgcgg agcagactga
      901 cgatcggggt tatgccgatg ccgcccgcga tgaacacgag acgttcagcg ccgggccgcg
      961 gccggtagga gccgtgcggc ccgtcgacca tcacgcgcgt gccgggcgcg agctgcgcga
     1021 tccggcggct gaagccgcca taggccttga tcgtgaacat gggccgctcc ggacggctgg
     1081 cgctggaggc gtacgagaag gggtgctcgg cgagcgcgtg cggcgcgtcg gccagcttca
     1141 gccaggcgaa ctgtcccggc cggaaggctc ggccgcggtg cccttccgcg cgcagcgcca
     1201 gcgtggtggc gccgccgcgc tcgggcacca gtcgctcgat caggtagggg cgcccggcca
     1261 gccgccgcgg gcgcagcagc cgaagctgga ccacggccag aaccgccagc gcggccagcg
     1321 cggccaccgt aaggccggcg ggcgagctca ggtagcgacc gacaccgagc gcgtgcagaa
     1381 tcgccagcgc cagcgcagag agccccagca ggaggtgcag gccgcgccag cgcgcgtaag
     1441 agagccgcaa ccgccggcgc agaacggagc tggcgacaag cgccgccagc gccgcggacg
     1501 aggcaaatgc cgcccgcgcc cgccagggcg ccccgagaag atcgaacagc gccaggtttg
     1561 cgggcgtgcc gacgaccacg agcgccacgt gcgcgacgat cgcggccacc agcaggtcgg
     1621 ccaggcggcg gtgtaaacgg atcgcaacct ccgcccccaa ccacgccgtc acggggcgca
     1681 ctcgcgccgg caacagcagc tggagcgcga gcaccgacaa cgccacgatc ccgaaacccg
     1741 accccagctc gatcacgaag ccacgccccg atggcggcga gccgaccagc gccaccgcca
     1801 acggaagcgt ggctgccccc gcgaatcccg caacccacaa acccttgaca gagcgcggca
     1861 cgtgcggccg agcagcagga aaccggcccg gccggcctgg ctcgccacgt gggcccgtat
     1921 cgatcacgcc gctgccttct tcagaacaga gacgcgggcc ctacaagtgg cctgcctgta
     1981 gcgcagcgca ttaagccggc tcgcgtgtgc agcagaccac agaccgcttc cgtcgcgggc
     2041 cgagccggtc ccgatctgct gctcaatgtg ggcaagctcg agttcagcca ggtgaagctc
     2101 gtcgcgtgtt gtggcttcct gcgccatcat ggggccaacg atcgcgcccg gtggtccggt
     2161 ccggtatggg gcaaatcccg caatcgcccc cgccccgtta ccgcggcctc acggctcctc
     2221 gatccccttg cccaaatggc gcaagggccg ggaatacccg gccctcgcat tcgcgtccca
     2281 cggcgtggta aatcaaccga ccttgaagct ccccttcagc gggatcgggt ggtggacctc
     2341 atgtcaggac ccgatcctga aacgtctcgc gcacgacgtt cgagatgggc ctaaggccac
     2401 cggccgggac ggtgacaccc tcaccggctg gctgcgggcc gggcgccgca ggcggtcgag
     2461 cgagcgcggg cagtcgtgtt tgatgcatga gggtgagctc cttttgttgt ggcgggccac
     2521 ccgccccgtc ggccgcctcg aacggtgttg caccttgcgg gcctgggggc atacgctcgc
     2581 caccggagat tccatgacga cggctgattc gcagcgggcc ggctcgcttg tcggacgtgc
     2641 gcgcgagctc gccgagctcg agcagacact cgaccgagtg gaatccggca cccactgggc
     2701 gatcgagatc gtcggcgagc cgggaatcgg caagtcgcgg ttgattgccg agctcggtcg
     2761 gatcgcggag gcacgcggat atctggtgct cgacggccgc gcggccgagt tcgagtcgga
     2821 catccctttc ggggtgatag tcgacgccct gaacgactac gtcgggtcgc tcgggccggc
     2881 ggtactgcgc gggctcgaag ccggtgtcgt gcaagagctc gcgttgatcc tcccctcgct
     2941 gtcgacgcta tccggcgagc aggtggggcc gcgggcgcag acggagcgct atcgggtcca
     3001 ctatgcgatc cgcgccttgc tcgagctgct tgccaagcgt cagccggtgc tactcgctat
     3061 cgacgatgtc cactgggcgg atgccgcctc attcgaggta atggcgcacc tcctgcgccg
     3121 cttccgcgga cctatgcttg cggctttcgc gtttcgttac gcgccagcgc gactggtcgg
     3181 ggcgctcgag gcgaccgcgc gggccggctt cggaagccgg ttggagcttg ccccgctgag
     3241 cgccgcagag gcggcgacgt tgctggatcc ggatctcgac ggcgcgaccc gtgacgcgct
     3301 ctacctcgaa agcgggggca acccgttcta tctcgagcag ctcgcgcggg gcccaaccgg
     3361 tcgcagtcct gcggcgtccg ggcggggcga gcgcctcggc gaggcgtggg agccgccccc
     3421 gggcgtggtc gccgcaatcg accaggagct ggatcgtctc agcgcgggcc aaaggcgcac
     3481 gctggacgcg gccgcggtcg ctggcgactc ctttgacccc ttgctggtct ccgcgattgc
     3541 ggagcaggac gagccgacgg cgctggcggc gctcgacggg ctgatcgagg tcggtcttgt
     3601 ccgacccaca gccgtgccgc gcagcttccg cttccgacac ccgatcgtgc gcagggccgt
     3661 gtacgacgcg ttgccgggcg gctggcgggt gggagcgcac gcgcgcgcgg cagccggcct
     3721 cgcctcgatt cacgcctcgg ggagcgagct ggcgcaccat gtcgctcgct cggccagcat
     3781 cggagacgag cacgcgatct cgctgctcat cgacgcggct cgcgccaccg ctgcacgggc
     3841 tccgctgacg gcgggacagt ggcttctcgc ggcggcgagg ctcgtgccca gcggggatta
     3901 cgagcgtcgc gttcgcctcc ttggggaggc gggcgcgtca ctcacctcag ggggcgggtt
     3961 cgatgacgcg ttgacgtcgc tggacgaggc cctctcgctt gcccacccgg attcaaccgg
     4021 cgttcgcgcc ggcctgatcg ccaaccgcgc cgaggcgagg cgccggggcg gccgtccttt
     4081 cgactcccgt ggcgagctcg agcgggcgct cgagtcaccg gccgggcccg aggggccgac
     4141 ggccattgcc gctcgactcg agctcgcgat ggatcgatac tggtacgccg atttcgccgc
     4201 ggttcatgag catgccgccc aagcgttggt cttcgcccga gaagacaggg atcccctcct
     4261 catctgcctc agtgcggcgc tcgatagcct cgcgagcagc tcgcgcaacc tcgccgatga
     4321 cgcgttcgcg cagttccgcg atgcgcaggc agcttacgcc gctcttccgg atgagcagtt
     4381 ggcggagcgg gtctacctct gccactacat gagtgaggcg gcgcttcgct tggagcgttc
     4441 cgacgatgcc ctggcccacc accgccgggg gtccgaggtc gcacggatga ccggccagga
     4501 tgccacgtcg cactcctggc ccgggctcgc ggtctacgcg ttgttgttga agggggagct
     4561 gaacgaggcg gcgcgcgttg cggcaggcgc catcaacact gcggacctgg cgagggacga
     4621 ctggaagatg atctgggtgc tggcggcgga cgcgctcgcg agcctatcga ccgggcagaa
     4681 cgagcgggcg ctcgccagcg cgcgcgagat gatcacgcgg gccgagcgaa gtcatcccgg
     4741 cacctccctg aacggcctag cgcgcgtgca gctcgccgcc gccctggcag ccgccggcga
     4801 cccggccggc gcgcacgccg agctcgtcgg gctggactcc gagcagagtc gctggctgct
     4861 cgacctgaat tgcggccaag ggtgggaggt gctcatcggc gcacggctcg ccgccggcga
     4921 tgtcgccgcc gcaggtgacg ctgctgccac agcagagtcg cgagtgaatt ccactcggct
     4981 gcagcagcgg acggccgcct tgcgtagcgc ccgcgcctcg gtaatgctcg cctcgggcga
     5041 cgcgcatgcg gccgccgagg cagcagaggc ggcggttcag attgcggagc ggaccggaaa
     5101 tccggttctg accggccgct gcctgagcca ggccggggcc acgctcgccg ctgcgggcca
     5161 gcgcgagagg ggagcccgac agctcgagcg tgccgagcgg acgttgtcgg cgtgcggggc
     5221 ggtgcgcgag gcggacgccg ccgcacgcga actccggcgc ctcggccgcc gcgtcccgcg
     5281 ccgggcgcgg ccgaaccgcg gcgtgggatt ggcggccctg agtccgcgcg aacgcgaggt
     5341 agcagaagag gtggccgcgg gcaagaccaa ccgcgacgtc gcggccacgc tttttctcag
     5401 cgagaagacg atagagagcc acttggtccg gatctattcc aagctcgacg tgcactcacg
     5461 gtccgcgcta accgccatcg tcgctcggca gagaggcgcc gccgacaggg cggccggccg
     5521 ccacagctga ccggaccttg aggcccgacc caccccggat tgggggtgcg attccagggt
     5581 tcgccccgta ccgcggtcgc acgccgcttg cgagccttgg cgcatgccca ccttcatcct
     5641 ctgtcacggc catcgacccg aggaatgtgc gatagcggtc gccgcctggc ggggatttcg
     5701 cagcccgctg cgtcacgggc gcccccttgg ctcgtgcgta actgggggac atcggctgtg
     5761 gtggacggtc gaagccgctg acgaagccgc cgcgctggga cagcttccgc cctacgtggc
     5821 cgagcgagcc atggtcgagc aggtccgcga ggtgccgctg ccatgagcgg agcaagccga
     5881 gcctcgttcg agtccgtcgc acttcagctc gcccccgcgg agcgccctgc caccaagtcc
     5941 gtggggccgt ccgagcaggg cctgcacgtc tgccccggct gcgactcgaa gctcgtctac
     6001 ccgacctcat gggaggagtg cccagacaac cgttggtatc tgacgctccg ctgcccggaa
     6061 tgcgagttca cgagcctagg ggaattcgag cagagcgcgg tcgatgagct cgagctagag
     6121 ctcgaacgcg gcgacgccga cctcgccgcc gacttgtccc tcttcatcca ggcgaacatg
     6181 gtcgacttcg tcaatcgctt cgtccgcgcg atcggcgcgg acgcaattca accgatggac
     6241 ttctgacatg gagccgcgac gtgcgggaac gctttgcaga ccacgggcgt cctcgtctaa
     6301 gccgctgaca gcgacacacc gggagcaaga catgcaccag atgcgacaca tcaagagcca
     6361 cactccggcg ggcatgacgg cgcccacgta ccggatcaat cgtccccggc gccactcggc
     6421 gatccgccgc ttcgccggct acggcatcgt caggtgaggt catcgcagcg atcgactatc
     6481 acgtctgaga aaccagggac acgacgacgc aacgactcct caatcccggt ccgcgaacgg
     6541 ggcggcatgt gggcaacgcc tgggcaacgt cacagccggg aatcgccggg gaacgcgtcc
     6601 gcccgtggaa ccacagaaag cccccactag cgagcaaaag gggaccagcc gggaacaccc
     6661 gggaacggtg ccactagatg gtaaggaacg ggtcatcggt tcaagtccga tagtcggctc
     6721 tctcagaaac ggccgccctg ccgaaaaccc atgccctgag cgcagatcgg acgcgatccg
     6781 accgcgctag actgacactt gacctcgcgc tccgggcggg gtcttcgttc gcccggggtg
     6841 catacaccgc cgagagggag caatggctcg cggagacgtt cgaattgggg ttacgctcgc
     6901 gtgcgaggag tgcaagcgac gcaactacca gactgagaaa agcaagcgga acgatccaaa
     6961 ccggatcgag ctgcgcaagt actgcccgtg gtgtggcaag cacacgcccc accgcgagac
     7021 ccgctgacgc gcagctcgtc gcgcgtcccg cctgagcatc cgtcatggca cgcaatcgtc
     7081 agagggccaa gcagcgccag gccgagcgcc gcgccgcgcg catagcgcag gccggcgccg
     7141 acacggcgcc gccgctgcct gagaaccccg accctgagct ggtcgaggag gccgcgcatc
     7201 tcgcggccgg cgcgccgccg gaggacgagg gccgcagcga caccgtgctc gaggagccgt
     7261 ccttcgagga ggaagaggag atcctcggcg agtccgaggc gcacgggcgc gagcgtgcgc
     7321 tcgagcggga tggacgctcc caccgcaggc acggtagagt catcgccttc ctgatagccg
     7381 tctgggcgga gctcaagcgc gtccaatggc cggaccggca gacgctcacc gtcctcaccg
     7441 gcgtcgtgtt gctcttcgtc ctgatcatgg gcggctatct cgggctactg gacgcagcgt
     7501 tctcgcgcct gatccaagcc atcctctaac acagactttc gagaaggaca cagatgtttc
     7561 gctggtacgc cgtcaacacc tactcaggac acgagaagaa ggtcaagcag aacctcgagc
     7621 accgcgtggt ttcgcttaac cagggccgca acgtgcgcca gatcgtgatc cccaccgagc
     7681 aggtttccga agtgaaggac ggccagaagg tgcagaccga gcgccgcacc atgccgggct
     7741 acgtgctcgt gaacatggac ctcaacgagg actcctggac gctcgtgaag gggacccccg
     7801 gcgtcaccgg tttcgtgggt ccgcagaaca agccggttcc gctcagccag ggcgaggtgg
     7861 accggctgct caaccgcgag gtcgccgagc gcccgcgcac gcgcgcgcag ttctcgatcg
     7921 gcgagtcggt caaggtcgtc tccggcccgc tgtccgactt ctcgggcgag atctcggaga
     7981 tcaacgagga cggcgccaag ctgaaggtgc tggtatctat cttcggtcgc gagacgccgg
     8041 tcgaagtggg cttcgaccaa gtgaagaaga tctagccgtg gcgaagaagg ttctgacact
     8101 catcaagctg caagttccgg gcggtgcggc caatccggct ccgccggtcg gccctgcgct
     8161 gggccagcac ggggtcaaca tcatggagtt ctgcaaggcg ttcaacgcgc agacccagga
     8221 ccagaacggc cgcatcatcc cggtcgagat cacggtctac gaggaccgct cgttcacctt
     8281 catcacgaag acgccgccgg ccgccgtgct gatcaaggag gccgccgggc tcgacaaggg
     8341 gtcagccgag ccgaaccgca cgaaggtcgc gaggatcacc cccgaccagg tgcgccagat
     8401 cgccgagacc aaactccagg acctgaacgc gcgcgacctc gacgccgcgt cgaagatcat
     8461 cgagggcacc gcccgctcga tgggagtgga ggtcggctga tgcccaagca cggcaagaag
     8521 taccgcgagg cttacgagaa cgtcgaccgc gagcgcgact acgagccgaa cgaggccgtc
     8581 tccctcgtca agtcgctgca gcgcgcggcc ttccctgaga ccgtggaggt gcacattcgc
     8641 accggcctga acgtacgcca cgcggatgag cagctgcgcg gcacgatcac gctgccccac
     8701 ggcggggggc gcgacgtgac gatcgccgtc ttcgccaagg gcgacaaggc gcgcgaggcg
     8761 caggacgccg gcgccgacgt ggtcggcgac gaggatctcg ccaagcgtgt cgaggagggc
     8821 ttcaccgact tcgacgtcgc gatcgccacg ccggacatga tgcccgtggt gggtcgcctc
     8881 ggccgcatcc tcggtcccca gggcaagatg ccgaacccga aggtcggtac ggtcacgaac
     8941 gacgtcgcgc aggcggtcga ggagtcgaag gccggcaagg tcgagtaccg caccgaccgc
     9001 tcggcgatcg tgcacctcgc gatcggcaag accgacttcg aggagcgtgc actgctcgag
     9061 aactatgcgg cgctgatcga cgagatcgtc cgagcgaagc cggccgcggc gaagggccgc
     9121 tatctgcga
//
LOCUS       DASVXS010000063         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1359533, whole genome shotgun sequence.
ACCESSION   DASVXS010000063 DASVXS010000000
VERSION     DASVXS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359533"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VF752_03470"
     CDS             <1..287
                     /locus_tag="VF752_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854635.1"
                     /translation="LDRVEQVRRQFPGVGFVGVVSGDDRAKVAKIVRERGLRYPVAVD
                     HDGELVNVYGIGVCPATVFAKPGGKVLVTKLGRLTDQQLSAQVRALLKAS"
     gene            284..925
                     /locus_tag="VF752_03475"
     CDS             284..925
                     /locus_tag="VF752_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEX8854636.1"
                     /translation="MSEPAIERGWVAAPLRDEFPFLGLMHVTLDVRPRKSPPEVRERL
                     REMSNRFTGGRAVQLRREPVPWAYRVFFRQIGIDPDHRRTPPEEAALERLRAGGFQTR
                     NLVDDALLIATVETGVALAAFDADRVQLPIGLRLAESGERLGGDGRDVPAGQIVIADQ
                     LRPLAVLFADMSDGVGVTPASRRMLIAAIRVKGVPEISVDEALWTVTEVVTGG"
     gene            979..1482
                     /locus_tag="VF752_03480"
     CDS             979..1482
                     /locus_tag="VF752_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854637.1"
                     /translation="MPELMQQHAPTQTPGFLIQPGIDERDARRTLLDQIARLEGELAS
                     LFCAAFPRPGFDWRVNSRGGPRILSLGELERVRDELAAKLQQNRRELSERTWIEEANR
                     RRIEQMLLDPAAHKWERVRNTDIGEQGCKQWHVLPRYGVIGMLMGWWRVKISSGCPLA
                     RGRGRRP"
     gene            1498..>2043
                     /locus_tag="VF752_03485"
     CDS             1498..>2043
                     /locus_tag="VF752_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854638.1"
                     /translation="MGRRSRKRASAGTGPEAVSTTGSTRAERDAARSRRAQALERGEA
                     PGRPHRVSARPRTRPKPPAPWGSFPLSELVILLGIVFVIAGLVVWGDRGRTLLVAGMV
                     LGSLGGLEVSIREHFAGYKSHTTLLAASTAVLSMILVSAIGGASGLAVLGIVVAVGVL
                     VFVLAFWGFRQAFMRRSGGLGF"
BASE COUNT          269 a          682 c          783 g          309 t
ORIGIN      
        1 agctcgatcg cgtggagcag gtgcggcggc aattcccggg cgtcggcttc gtgggcgtgg
       61 tgagcggcga cgaccgcgcg aaggtggcga agatcgtgcg cgagcgcggc ctgcgctacc
      121 cggtcgcggt cgatcacgac ggcgagctcg tgaacgtcta cggcatcggc gtctgcccgg
      181 cgacggtgtt cgcgaagccg gggggcaagg tgctggtgac gaagctgggc aggttgacgg
      241 atcagcagct gagcgcgcag gtgcgcgcgc tgctgaaggc gtcgtgagcg agccggcgat
      301 cgagcgcggc tgggtcgcag cgccgctgcg cgacgagttc ccgttcttgg gactcatgca
      361 cgtgacgctc gatgtgcggc cccgcaagag cccgcccgag gtgcgcgagc ggctgcggga
      421 gatgagcaac cgcttcaccg gtgggagggc cgtgcagctc cgccgcgagc ccgtgccgtg
      481 ggcgtaccgc gtcttcttcc gccagatcgg gatcgatccc gaccatcggc gcacgccccc
      541 cgaggaggcg gcgctcgaac gcttgcgcgc aggcggcttc cagacgcgca acctcgtcga
      601 cgacgcgctg ctgatcgcca cggtggagac cggcgtggcg ctcgcggcgt tcgacgccga
      661 ccgcgtgcag cttccgatcg gcctgcgcct cgccgagtcc ggcgagcggc tcggcggcga
      721 cggccgcgac gtcccggcgg gccagatcgt gatcgccgac cagctccgcc cgctcgcggt
      781 gctgttcgcc gacatgtcgg acggggtcgg agtcacgccg gcctcgcggc gcatgctgat
      841 cgccgcgatc cgtgtgaagg gcgtgcccga gatcagcgtg gacgaggcgc tctggaccgt
      901 cacggaggta gtgacaggtg gatgaggggt accaagttgg gtggtacgat cgaggcaacg
      961 agtggggagg aaagcgccgt gcccgagctg atgcagcaac acgcgccgac gcagacaccc
     1021 ggtttcctga ttcagccggg gatcgacgag cgcgacgccc ggcggactct tctcgaccag
     1081 atcgctcggc tcgagggcga gttggcgtcg cttttctgcg ccgccttccc gcgcccgggc
     1141 ttcgactggc gtgtgaactc gcgcggcggg ccacgcatcc tctcgctggg ggagctcgag
     1201 cgggtgcgcg acgagctcgc ggcgaagctc cagcagaacc gccgggagct ctccgagcgc
     1261 acctggatcg aggaggcgaa ccggcgtcgc atcgagcaga tgctgctcga tcctgccgcg
     1321 cacaagtggg agcgcgtgcg caacaccgac atcggcgagc agggttgcaa gcagtggcac
     1381 gtgctgccgc gctacggcgt cattggcatg ctgatggggt ggtggcgcgt gaagatctcc
     1441 tccggttgtc cgttagccag ggggcgcggc cggcggccgt gaccccgttc cgggcagatg
     1501 ggtaggcgaa gccgcaagcg cgcctcggcc ggtaccggcc cagaggccgt ctcgacgacg
     1561 gggtcgacgc gcgccgagcg cgacgccgcg cgcagccgcc gcgcccaggc cctcgagcgc
     1621 ggagaggcac ccggccgccc gcaccgcgtg agcgcgcggc cccgcacgcg gccgaagccg
     1681 ccggcgcctt ggggctcgtt ccccctaagc gagctggtca tcctcctcgg gatcgtcttc
     1741 gtgatcgccg ggctcgtggt ctggggcgac cggggccgca cgctgctggt ggctggcatg
     1801 gtgctcggct cgctcggcgg gctcgaggtg tcgatccgcg agcacttcgc cggctacaag
     1861 tcgcacacga cgctgctggc cgcgagcacc gccgtgctgt cgatgatcct cgtctcggca
     1921 atcggcggcg cgagcgggct cgccgtcctg ggtatcgtcg tggccgtggg cgtgcttgtg
     1981 ttcgtgcttg cgttctgggg cttccgccag gccttcatgc gccgttcggg cgggctcggg
     2041 ttc
//
LOCUS       DASVXS010000064        12704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1288395, whole genome shotgun sequence.
ACCESSION   DASVXS010000064 DASVXS010000000
VERSION     DASVXS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288395"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VF752_03490"
     CDS             <1..464
                     /locus_tag="VF752_03490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8854639.1"
                     /translation="LTGVGNYRTLYERLAYEIARHERHNRRFVLTLLDLDRFKQVNEE
                     HGHLEGDRVLREVGRVLAFSVREQDTLARQGGDEFSVLAPEADASDAEQLAARLSAAL
                     ASIQVDGRPLTASIGWAVFPEDGVSPDALVARADHALLAGKRDRRSRQYAV"
     gene            complement(461..994)
                     /locus_tag="VF752_03495"
     CDS             complement(461..994)
                     /locus_tag="VF752_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX8854640.1"
                     /translation="MPAGRFVEALNEQIGNEFGASQSYAAMAVHYDAETLPQLATFFY
                     RQSVEERNHAMMMVRYLLDAGAEVTMPGVEPPRTQFADIVEPVAAALEQEKRVSDQIE
                     RLAQVAREQNDFTSEQFIQWFLKEQVEEVATMSALLAVAQRSRDNAMLVEDFIAREHS
                     GREDSDPTAPPAAGGAL"
     gene            1081..1884
                     /locus_tag="VF752_03500"
     CDS             1081..1884
                     /locus_tag="VF752_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8854641.1"
                     /translation="MPLDLSGQRALITGASGGIGNAIARELHSRGAHVILSGRRADAL
                     ESLAGDLGPRAEVIAADLADAFDVRSLLERAGKVDVLVANAALPGSGEVDSFSPEEID
                     RAIDVNLRAPIQMARELVPGMVERGRGHLVFVSSLSGKVAALGTSVYSATKFGLRGFA
                     FSLHEDLRGTGVGVTTVFPSFIRGAGMWADTGLELPAGVRTRTPEQVAAAVVRGIDKN
                     VAEIDVAPVSLRLGGWLFGFAPGVTAALSRLGGGRKLSAATARAQLGKR"
     gene            complement(2489..6787)
                     /locus_tag="VF752_03505"
     CDS             complement(2489..6787)
                     /locus_tag="VF752_03505"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX8854642.1"
                     /translation="MSTRTTVQITMPQMGESVTEGTILEWRKQVGEAVEQDEPLVDVS
                     TDKVDTEIPSPASGTLVSISVGADETVPVGTVIGEIAVGDGEDGAPAPAAAPPDSGNG
                     AAAPEQPAAASPERPAAASGELVDLVFPQMGDSVAEGTILEWTKQVGDQVEAEETVVE
                     ISTDKVDAEVPSPVSGTIAEILVQADETVSVGTVLARIAAGAGAPAPAPQATPPSAET
                     AALEGQPQPRPSAGNGAENATPVAARMASAHGLDVNELSGSGPRGRVTKSDVEAAIGG
                     NGAGAPAPAAAAPAPSVPGVKPIRGPAAMLAKFMNESRSIPTATSFRTLSVDELDRRR
                     KALKGAGKRLSFTHLIAWAIVDAAREMPVMGNAYAEVDGKPQRVEPATVSLGIAVDVE
                     RRDGTRSLVVPVIRDASEMDFPSFIARYDELVAGARDNTLAPDDYQGANVSLTNPGGI
                     GTVASVPRLMPGQGTIVATGAIGYPPGLQQVAPERLTELGVSKVMTMTSTYDHRVIQG
                     AESGAFLRRIDQLLQGDDNFYESVFASVGVDPGAPALDGAGPVTADEPAPSTVTPDVV
                     GKPDEALLQAVQAATSIVKAHRMHGHLSARLDPLGSEPVGDPALDPTTVHLTPELMRA
                     IPASVLRVAVPGETFADALPHLQETYCGTIAYEIEHISDHEQRVWLRQTIESGEHRTP
                     LTADEKRALLERLSQVEALETYLHKAFLGKKQFSVEGLDMLVPMLDETIEESAEAGSR
                     ETLIGMAHRGRLNVLAHTVGRPYETILVEFEGEGSLDADTAKPEEGTGDVKYHYGASG
                     TYKTREGKGVTVSLSHNPSHLEYVNPVVEGRARADQTSRKGREEHHDPTVVLPVLIHG
                     DAAFPGQGVVSETLNLQALAGYATGGTLHIIANNQVGFTTDPWEGRSTRYASDLAKGF
                     DIPIIHVNADDAEACIAAVRLAMEFRHRFGRDALIDLIGYRRFGHNETDEPAYTQPQM
                     YEAIKKHPTVRKIYAELLTEQGVVTAEEAEQMATAAYQAVADAHNELKASMAAPGPDT
                     GEFQLDRSASAEPRTAVPAEQLLSLNEQLLKVPEGFNIHRKLKPQVERRRDGVADGAI
                     DWAQAESLAWASLLTQGVPIRLTGQDSERGTFSQRHLVFHDAKSGERHTPIQNLPNAT
                     VPFELYNSPLSETACLGFEYGYSVQAPEALVLWEAQFGDFVNSGQVIVDQFIASGLAK
                     WGQSSRLTLLLPHGYEGSGPEHSSGRLERFLQLAAEGNMRVANCTTPAQYFHLMRRQA
                     LVSKERPLVIMTPKSLLRLPAAASQLEDLSEGSFQPVIDDATLPGSRDEVTRLVLCTG
                     KVYYDLVSHEERANARNVAVARVELLYPFAASEIQALMASYPSLREVVWVQEEPKNMG
                     ARAFMKPRLDEILPDPIAYDYVGRELRASPGEGYPAAHRAEQNRIVRAALKTAS"
     gene            6847..8421
                     /locus_tag="VF752_03510"
     CDS             6847..8421
                     /locus_tag="VF752_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8854643.1"
                     /translation="MKFWGWGFEDQQPSHEQVAEAAAGIRAHVGFGDGEVERPPRLED
                     LELPAPRVEPPASLARICSSDVHERASHAYGKAYRDVVRSFRGRIDHPPDVVARPTRE
                     PEVEALLEWCEQARLAAIPFGGGTSVVGGVEPRVGGDYAGTVSIDLKALDRVLEVDPV
                     SRAARIQAGATGPLLEDQLREHGLTLRHYPQSFEYSTLGGWIATRAGGHFATLYTHID
                     DLVESVRAITPRGTWESRRLPASGAGPSPDRLLLGSEGILGVITEAWVRVQERPRFRA
                     SAGVHFPGFEEGAAAVRALAQSGLHPSNCRLLDPTEAEITGAAPGGVALLVLGFESAD
                     HELGPWMARALELCGDHGGRAVEEKSGSGDGTGAWRDVFLQAPYLRDTLVAAGIVADT
                     FETAITWDRFGGFVAQVRETAERAVASECGAGRVSCRLTHVYPDGAAPYFTVLAPARR
                     GSEIEQWDAIKRAASEAVEAAGGTITHHHAVGRDHRPWYDCQRPEPFAAALRAAKGSV
                     DPAAILNPGVLIEPLA"
     gene            8418..9422
                     /locus_tag="VF752_03515"
     CDS             8418..9422
                     /locus_tag="VF752_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8854644.1"
                     /translation="MSTPRRKRELARADQVMRGLWRLRLPIVVWPGVPHVNAFAIAAG
                     NGVVLVDTGLAEAGGLGRLERALDQAGLNLEHVRLLVCTHAHSDHYGLAQPIVEASGA
                     ELWMHPNHEHMTKAAANPERSFERRIEVARQSGVPEEAIRRYEEQRKSRGYGIAGIVL
                     PDRDLVPGVKVETDLGAWDVYETPGHAPSHVVLHQPERGLLLSGDHLLGRVSLYYDYG
                     WSPDPAGEFLSSLDVVDELDVGLCLAGHGRPFRDVKGHIQANRQLVAEKVGRVRAAID
                     GEPRTAYEIVPFLLGEDSPSQLMMIWGMSETLVYLRHMERLGEAEKVDSEPERWRLVA
                     "
     gene            9590..11233
                     /locus_tag="VF752_03520"
     CDS             9590..11233
                     /locus_tag="VF752_03520"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HEX8854645.1"
                     /translation="MGSQAKLLRTLMATALAVIGVFAFASVAQADTYTVTKEADTADG
                     TCDSDCSLREAVIAANSTEADDTIVLDPGHYNLTIEGSEEDQAADGDLDLLDNGSITI
                     TGTSARNTIIDGLGTADADNLIESERVFDVYARASISNVTIQGGSQFGGGGGIRIRGL
                     MGPIQTPAGFVGSASLSLDHVSVSGNEVSGSGGGIENDGGDLTISRSLLSNNFAESDG
                     GAVVSYDGATTHIENSTIQDNTADSNAGCAGIGAVTGFNADITLLNDTIVDNVNLDGT
                     CSADPTAGLASEDFSSDGVSEEPSGSMTVQNTVVANNQTLGPVEGEGTLSVGGTPELN
                     NCAGPGITSAGYNIEDDTDCSFANTGDLQNTDPKLGGLDNNGGQTDTRALLDGSPAIN
                     TAGPGAPATDQRDVSRPQGAASDIGAFEVEVPAPPTTQPVQPVQQQQKPGAPKLAVAG
                     VRRACTSSARVSVKVKATVASGTTLKQVRVTLDGKRLYTSKKGTFTLKVNATRLKAGR
                     HRLRVTVLDSAGGSKTTTKVLARCAQAKPKAKVQPRFTG"
     gene            complement(11328..12245)
                     /gene="metF"
                     /locus_tag="VF752_03525"
     CDS             complement(11328..12245)
                     /gene="metF"
                     /locus_tag="VF752_03525"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX8854646.1"
                     /translation="MRIDRILDDRYPCFSFEFFPPKTDEGMENLFNAVAELKHDEPGF
                     VSVTYGAMGSTRDRTIEITKRIKRDLGVEAMSHLSCVGTTREELRAILDEIGEAGIEN
                     VLALRGDPPQGETEWKPTPGGLKYSTELAELITQNYEYAVGAACFPEVHPEAADMESD
                     LRFLKKKVGAGARFLITQLFYDNELYFNFVDKAREFGIDVPIIPGIMPITNYQQIKRI
                     TGMCGATIPAELERELSARQENPAAVQDLGVAYATLQSADLLARGAPGIHFYTLNRSP
                     ATRAILSALRAWRPWEGGERPKPVLAAAG"
     gene            complement(12274..12525)
                     /locus_tag="VF752_03530"
     CDS             complement(12274..12525)
                     /locus_tag="VF752_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854647.1"
                     /translation="MFVNGVPQQEGTDYRLEGRELVFERRLTKEGRLGFWRWTSIFLG
                     LIGTYRQNDSVDVQYRLHGRERVATGLDIVPPPDDQPQG"
BASE COUNT         1808 a         4417 c         4486 g         1993 t
ORIGIN      
        1 cgctgaccgg cgtaggcaac taccgcacgc tgtacgagcg gctcgcctac gagatcgccc
       61 ggcacgagcg ccacaaccgc cgcttcgtgc tcacgctgct cgatctcgac cgcttcaagc
      121 aggtgaacga ggagcacggg cacctcgagg gcgaccgcgt gcttcgcgag gtcggccgcg
      181 tgctcgcctt cagcgtgcgc gagcaggaca cgctcgcacg ccagggcggc gacgagttct
      241 cggtgctcgc ccctgaggcc gacgcaagcg acgccgagca gctcgcagct cgcctgagcg
      301 ccgcgctcgc ctcgatccag gtcgacggtc gcccgctcac cgccagcatc ggctgggccg
      361 tgttccccga ggacggcgtg agcccggacg cgctcgtggc gcgcgccgat cacgcgctcc
      421 tcgcgggcaa gcgcgaccgg cgcagccgcc agtacgcggt ctagagcgcg cctccagcgg
      481 ccggcggcgc cgtcggatcg ctgtcctcgc ggcccgagtg ctcgcgggcg atgaagtcct
      541 ccacgagcat cgcgttgtcg cggctgcgct gcgccaccgc gaggagcgcc gacatcgtgg
      601 cgacctcctc gacctgctcc ttgaggaacc actggatgaa ctgctcgctc gtgaagtcgt
      661 tctgctcgcg ggcgacttgg gcgagccgct cgatctggtc gctcacgcgc ttctcctgct
      721 cgagcgcggc ggcgaccggc tcgacgatgt cggcgaactg cgtgcgcggg ggctccacgc
      781 ccggcatcgt gacctccgcg cccgcgtcga gaaggtaccg gaccatcatc atcgcgtggt
      841 tccgctcctc taccgactgg cggtagaaga aggtggcgag ctggggcagc gtctcggcgt
      901 cgtagtggac ggccatcgcg gcatacgact gcgacgcgcc gaactcgttt ccgatctgct
      961 cgttgagagc ctcgacgaag cgtcctgcgg gcatccgacc tcctggaaag acgatggcgt
     1021 gcggggatac gatctcacgc gaatgccgcc cgggcgtcct cacccaacgg agcgaagccg
     1081 gtgccgctcg acctgagcgg ccagcgggcg ctcatcacgg gcgcctcggg cggcatcggg
     1141 aacgcaatcg cgcgggagct tcatagcagg ggcgcccacg tgatcctcag cggacggcgt
     1201 gcggacgcgc tcgagtcgct ggccggcgat ctcggccctc gtgcggaggt gatcgcggcc
     1261 gatctcgcag acgccttcga cgtgcgctca ctactcgagc gagccggcaa ggtcgacgtg
     1321 ctcgtggcga acgccgcgct gccgggttcc ggtgaggtcg acagcttctc gccggaggag
     1381 atcgaccggg ccattgacgt gaacctgcgc gcgccgattc aaatggcgcg cgagctcgtg
     1441 cccgggatgg tggagcgcgg gcgtgggcat ctcgtgttcg tgtcgtcgct gtccggcaag
     1501 gtcgccgcgc tgggcaccag cgtctacagc gcgacgaagt tcgggctgcg cggcttcgcc
     1561 ttctcgctgc acgaggacct gcgcggcaca ggcgtgggcg tcacgacggt gttcccgagc
     1621 ttcatccgtg gcgccggcat gtgggctgac acggggctcg agctgccagc cggcgtgcgc
     1681 acgcgcacgc ccgagcaggt cgcagcagcc gttgtccgcg gaatcgacaa gaacgtcgcg
     1741 gagatcgacg tggcgccggt ctcgctgcgc ctcggcggct ggctgttcgg attcgcgccc
     1801 ggagtgacgg ccgcgctctc gcgcctcggg ggcggccgca agctctcggc cgcgaccgcg
     1861 cgcgctcagc tcggaaagcg ctgacctcga tcgagctgtt cgggaagcgg cggcggccgc
     1921 cgcttaggca gcatttcgtc gggaagctcg cttccgccca ccgaaaccgg acgttttgcg
     1981 cgccacaccc gcatttcgtc ggttggcgtc tcgccgaatc gcggttcccg acctttcgcg
     2041 cgtacgtggc ggacagctcc gagaagggcg gccattccgc gactccggaa ccgctatcgc
     2101 ggtttagccg gacactggct gggaatcacg gtcgttgcag ggggagcttc tcggacgctg
     2161 cgcgggggat tggcgcgtcc acaaggtttt tcgaaacggc tctggcgggc gcggctggta
     2221 gctatgcggg gggagctagc gatccagccc ccgccggggc ctgatggcgt cgcctggcgg
     2281 cgtcctcgcc ccctcgcgtg cagagcacgc cacgggggct gcgtccttgc catgctcgcc
     2341 ctcagacccc ggcgccgccg gggccctcgg ccctcagtgt ccgtctcgct ccggcgagcg
     2401 gccgaacggg ggatgagatc ctgctcgacc ccctgttatt gctgctgttc gggccccggg
     2461 cgttgagagc tcgaacgagt cgaaggcctc aggacgccgt cttcagcgcc gcgcgcacta
     2521 ttcggttctg ctccgcgcgg tgcgccgccg ggtagccctc gccgggggag gcgcgcagct
     2581 cgcggcccac gtagtcgtag gcgatcgggt cgggcaggat ctcgtcgagg cgcggcttca
     2641 tgaacgcgcg cgcgcccatg ttcttcggct cctcctgcac ccacacgacc tcgcgcaggc
     2701 tggggtagct cgccatcagc gcctggatct cgctcgcggc gaacggatag agcagctcaa
     2761 ctcgcgcgac cgccacgttg cgagcgttcg cgcgctcctc gtgggagacg aggtcgtaat
     2821 agaccttgcc ggtgcagagc acgaggcgag tgacctcgtc gcgtgagccg ggaagcgtcg
     2881 cgtcgtcgat caccggctgg aacgagccct cgctcaggtc ctcgagctgc gaggcggcgg
     2941 ccggcaggcg cagcaggctc ttcggagtca tgatcacgag cgggcgctcc ttcgacacca
     3001 gcgcctggcg gcgcatcagg tggaagtact gcgcgggcgt ggtgcagttt gcgacgcgca
     3061 tgttgccctc ggctgccagc tgtaggaagc gctccaggcg gccgctcgag tgctcggggc
     3121 ccgagccctc gtagccgtgg ggaagcagca gcgtgagccg ggacgactgg ccccacttcg
     3181 caaggcccga ggcgatgaac tggtcgacga tcacctggcc cgagttcacg aagtcgccga
     3241 actgcgcctc ccagagcacg agggcctcag gggcctgcac gctgtagccg tactcgaaac
     3301 ccaggcacgc ggtttccgac agtggcgagt tgtaaagctc gaacgggacc gtggcgttcg
     3361 gcagattctg gatcggcgtg tggcgttcgc cgctcttcgc gtcgtggaac accaggtggc
     3421 gctgactgaa cgtgccccgc tcggagtcct ggccggtgag acggatcggc acgccctgag
     3481 tgagcagcga cgcccacgcg agcgactccg cctgcgccca atcgatggcg ccgtctgcga
     3541 cgccgtcgcg tcgccgctcg acctgcggct tgagcttccg gtggatgttg aagccctccg
     3601 gcaccttgag cagctgctcg ttcagcgaca ggagctgctc ggccggcacc gccgtgcgcg
     3661 gctcggcgct cgcggagcgg tcgagctgga actcgccggt gtccggcccg ggcgcggcca
     3721 tcgaggcctt cagctcgttg tgcgcgtcgg ccaccgcctg gtaggccgcc gtcgccatct
     3781 gctcggcttc ctcggcggtc acgacaccct gctccgtgag cagctcggcg tagatcttcc
     3841 gaaccgtcgg gtgcttcttg atcgcctcgt acatctgcgg ctgggtgtac gcaggctcgt
     3901 cggtctcgtt gtggccaaag cggcggtagc cgatcaggtc gatcagcgcg tcgcggccga
     3961 agcggtggcg gaactccatc gccagccgga cggcggcgat gcaggcctcg gcgtcgtcgg
     4021 cgttcacgtg gatgatcggg atgtcgaagc cctttgcgag gtccgacgcg taacgggtgg
     4081 agcgcccctc ccacggatcg gtcgtgaagc caacttggtt gttggcgatg atgtgcagcg
     4141 tgccgccggt ggcgtagccg gcaagcgcct gcaggttgag cgtctcggag acgacgccct
     4201 ggccggggaa ggcggcgtcg ccgtgaatca gcacgggcag cacgacggtc ggatcgtggt
     4261 gctcctcgcg gcccttgcgg ctcgtctgat cggcgcgcgc gcggccctct accacggggt
     4321 tcacgtactc gaggtggctt gggttgtgcg agagcgagac cgtcacgccc ttgccctcgc
     4381 gcgtcttgta ggtgcccgag gcgccgtagt ggtacttcac gtcgccggtg ccctcctcgg
     4441 gcttggcggt gtcggcgtcg agcgagccct cgccctcgaa ctccacgagg atcgtctcgt
     4501 acggccgccc gacggtgtgc gccagcacgt tcaggcgccc gcgatgggcc atgccgatca
     4561 gcgtctcgcg cgagcccgcc tccgcggact cctcgatcgt ctcgtcgagc atcggcacga
     4621 gcatgtcgag cccctccacc gagaactgct tcttgccgag gaacgccttg tgcaggtagg
     4681 tctcgagcgc ctccacctgt gagagtcgct cgagcagcgc gcgcttctcg tcggcggtca
     4741 gcggcgtgcg gtgctcgccc gactcgatcg tctggcgcag ccatacgcgc tgctcgtggt
     4801 ctgagatgtg ctcgatctcg tacgcgatcg tgccgcagta ggtctcctgc aggtgcggca
     4861 gcgcgtcggc gaacgtctca ccgggcaccg ccacgcgcag caccgacgcc gggatcgcgc
     4921 gcatcagctc gggcgtgagg tgcactgtgg tggggtcgag cgccgggtcg ccgacgggtt
     4981 ccgatcccag cgggtcgagc cgggcggaga ggtgcccgtg catgcggtgc gccttcacga
     5041 tcgaggtggc cgcctgcact gcctggagga gcgcctcgtc cggcttgccg acgacgtcgg
     5101 gcgtgacggt cgacggtgcg ggctcgtcgg cggtgaccgg gccggcgccg tcgagcgcgg
     5161 gtgcgcctgg gtcgacgccc accgatgcga acaccgactc gtagaagttg tcgtccccct
     5221 gcagcagctg gtcgatcctg cgcaggaatg cacccgactc cgcgccctgg atcacgcggt
     5281 ggtcgtaggt gctcgtcatc gtcatcacct tgctcacgcc aagctcggtg agccgctcgg
     5341 gcgcaacctg ctgcagaccg ggcgggtagc cgatggcgcc ggtggcgacg atcgtgccct
     5401 ggccgggcat cagccgcggc acggaagcca ccgtgccgat gccgccgggg ttcgtgaggc
     5461 tgacgttcgc gccctggtag tcgtccggcg cgagggtgtt gtcgcgtgcg ccggccacca
     5521 gctcgtcgta gcgagcgatg aaggacggga agtccatctc gctggcgtcg cggatgaccg
     5581 gcacgaccag cgagcgcgtg ccgtccctgc gctccacgtc gaccgcgatt cccaggctca
     5641 cggtggcggg ctcgacgcgc tgcggcttgc cgtcgacctc ggcgtaggcg ttgcccatga
     5701 ccggcatctc gcgcgccgcg tcgacgatcg cccaggcgat caggtgcgtg aacgagaggc
     5761 gcttgccggc tcccttgagc gccttgcggc gccggtccag ctcgtcgacg gagagcgtgc
     5821 ggaagctcgt ggccgtcggg atcgagcggc tctcgttcat gaacttggcg agcatcgccg
     5881 ccggtcctcg gatcggcttc acgccgggca cgctgggcgc cggtgctgcc gcggcaggtg
     5941 caggcgcgcc ggcgccgttg ccgccgattg cggcctcgac gtcgctcttc gtcacgcgcc
     6001 cgcggggacc actgccgctc agttcgttca cgtcgaggcc gtgggcgctc gccatgcgcg
     6061 ctgcgacggg cgtcgcgttc tcggcgccat tgcccgcgct cgggcgtggc tgcggctggc
     6121 cctcgagcgc tgcggtttcg gcgctcggcg gcgtggcttg cggtgcgggc gctggcgcgc
     6181 cggcgcccgc cgctatccgc gcgagcacgg tgccgaccga cacggtctca tcggcctgca
     6241 cgaggatctc cgcgatcgtc ccggacaccg gagatggcac ctcggcgtcg accttgtcgg
     6301 tggagatctc gacgaccgtc tcctcggcct ccacctgatc ccccacctgc ttggtccact
     6361 cgaggatcgt gccctcggca acggagtcgc ccatctgcgg gaagaccagg tccacgagct
     6421 cgccgctcgc ggcggcgggc cgctcggggg acgcggcggc gggctgctcg ggcgcggcgg
     6481 caccgtttcc gctgtccggc ggggccgcgg cgggagcggg cgcgccatcc tcgccgtcgc
     6541 cgacggcgat ctcgccgatc accgtgccga ccggaacggt ctcatccgcc ccgaccgaga
     6601 tcgagacaag cgttccggaa gccggcgagg ggatctctgt gtcgaccttg tcggtcgaga
     6661 cgtcgacgag cggctcgtcc tgctcgaccg cctcgccgac ctgcttgcgc cactcgagga
     6721 tcgtcccctc ggtcaccgac tcgcccattt ggggcatggt gatctggacg gtggttcgcg
     6781 tcgacatgtg cccgccgcgt aactctagag cgtgcgacag ttcccggccg gatgaattcg
     6841 cgccgaatga agttctgggg ctggggcttc gaggaccagc agccgtcgca cgagcaggtg
     6901 gccgaggcgg ccgccggcat ccgggcgcac gtcggcttcg gcgacggcga ggtcgagcgc
     6961 ccgccgcggc tggaggatct ggagctgccg gcgccgcgcg tcgagccacc cgcctcgctc
     7021 gcccggatct gctcgagcga cgtccacgag cgggcctcgc acgcctacgg caaggcgtac
     7081 cgcgacgtgg tgcgctcctt tcgcggccgg atcgaccatc caccagacgt cgtggcccgc
     7141 ccgacgcgcg agccggaggt ggaggccctc ctcgagtggt gcgagcaggc gcggctggcg
     7201 gcgatcccgt tcggcggcgg caccagcgtg gtgggcggcg ttgagccgcg cgtgggcggc
     7261 gattacgccg ggacggtctc gatcgacctg aaagcgctcg atcgcgtgct cgaggtggac
     7321 ccggtgtcgc gcgccgcgcg catacaggcg ggcgcgaccg gtcccctact cgaggaccag
     7381 ctgcgcgagc acgggctcac gctgcgccac tacccgcagt cgttcgagta ctccacgctc
     7441 ggcggctgga tcgccacccg cgcgggcggc catttcgcca cgctctacac gcacatcgac
     7501 gacctggtgg agtccgtccg cgcgatcaca ccgcgaggaa cctgggagag ccggcgcctg
     7561 ccggcgtcgg gcgccggccc gagccccgac cggctgctgc tcggctcgga gggcatcctc
     7621 ggcgtgatca ccgaggcgtg ggtgcgggtt caggagcgac cgcgcttccg ggcgtcggcc
     7681 ggagtccact tcccgggttt cgaggagggt gcggcggcgg ttcgcgcgct cgcgcaatcc
     7741 ggcctgcatc cctcgaactg ccgcctgctc gaccccacgg aggcggagat cacgggcgcc
     7801 gcgcccggtg gcgtggcgct gctcgttctg ggcttcgagt cggccgacca cgagctcggc
     7861 ccgtggatgg cgcgcgcgct cgagctctgc ggcgatcacg gcgggagagc ggtcgaggag
     7921 aagagcggtt ccggcgacgg cacgggcgcc tggcgcgacg tgttcctgca ggcgccttac
     7981 ctgcgcgaca cgctcgtggc ggcgggcatc gtggccgaca cgttcgagac cgcgattacc
     8041 tgggaccgct tcggcggctt cgtcgcccag gtccgtgaga ccgcggagcg agcggtggcc
     8101 tccgagtgcg gcgcgggccg cgtcagctgc cggctgacgc acgtctatcc cgacggggcc
     8161 gcgccgtact tcacggtgct cgcgcccgcc cggcgcggct ccgagatcga gcagtgggac
     8221 gcgatcaagc gtgcggcgag cgaggccgtc gaggcggcgg gcgggactat cacccaccac
     8281 cacgcggttg ggcgcgacca ccgcccttgg tacgactgcc agcggcccga gccgttcgcc
     8341 gcggcgctgc gcgcagccaa gggctcggtc gaccccgctg ccattctcaa tcccggcgtc
     8401 ctgattgagc ccctcgcgtg agcaccccgc gccgaaagcg cgagctcgct cgcgccgacc
     8461 aggtgatgcg cggactctgg cggctacgcc tgccgatagt cgtgtggccc ggagtaccgc
     8521 acgtcaacgc ctttgcgatc gccgcgggca acggcgtcgt gctggtcgac accggcctgg
     8581 cggaggcagg agggctcggc cggctcgagc gcgcgctcga ccaagccggt ctgaatctcg
     8641 agcacgtgcg cttgctcgtg tgcacgcacg cgcactccga ccactacggc ctcgcgcagc
     8701 cgatcgtgga ggcgtcgggc gccgagctct ggatgcatcc gaaccacgag cacatgacga
     8761 aggcggccgc caatccggag cgctcattcg agcgccggat cgaggtggcg cgccagagcg
     8821 gcgtaccgga ggaggcgatc cgccgctacg aggagcagcg aaagagccgg ggctacggaa
     8881 tcgcggggat cgtgctcccg gaccgcgacc tcgtgcccgg ggtgaaggtc gagaccgacc
     8941 tcggcgcctg ggacgtctac gagacgcccg gccacgcgcc gtcgcacgtg gtcctgcacc
     9001 agcccgagcg cgggctgctc ctctcgggcg accacctgct cgggcgcgtc tcgctctact
     9061 acgactacgg ctggtcaccc gaccccgcag gtgagttcct gagctcgctc gacgtcgtcg
     9121 acgagctcga cgtgggcctg tgcctcgccg ggcacggccg ccccttccgc gacgtgaagg
     9181 gacacatcca ggctaatcgc cagctggtcg cggagaaggt cgggcgtgtg cgggccgcga
     9241 tcgacggcga gccgcgcacc gcttacgaga tcgtgccgtt tctgctcggt gaggacagcc
     9301 ccagtcagct gatgatgatc tggggcatgt ccgagacgct cgtctacctg cgccacatgg
     9361 agcgcctcgg cgaggcggag aaagtcgact cagagcctga gcgctggcga ctggtcgcat
     9421 gagttgtcgc ccgttcgggt ggccgcacca ttccctgtac gggtagcacc cgaatcctca
     9481 tctggacgcc tctgcggccc caaaggaggt gtcgaaaccg cttgacgggg gtcccctggc
     9541 gggcttagga catccgttga ggatttcttc cctaaaggag gcccaagaag tgggatcgca
     9601 agccaagcta ctgcgcacgc tgatggcgac cgcgctcgcc gtgatcggcg tgtttgcatt
     9661 cgcgtccgtg gcgcaggctg acacctacac ggtgaccaag gaggccgaca ccgccgacgg
     9721 cacctgcgac agcgactgct cgctgcgcga ggccgtcatc gcggccaact cgaccgaggc
     9781 cgacgacacg atcgtgctcg accccggtca ctacaacctg acgatcgagg ggtcggaaga
     9841 ggaccaggcc gccgacggtg acctcgatct gctcgacaac ggctcgatca cgatcacggg
     9901 cactagcgcc cggaacacga tcatcgatgg gctcgggacc gccgacgcgg acaacctgat
     9961 cgagtccgag cgcgtgttcg acgtctacgc ccgtgcatcg atctcgaacg tgacgattca
    10021 gggcggcagc cagttcggcg gcggcggcgg catccgtatt cgcggcctga tgggcccgat
    10081 tcagacgccc gccggcttcg tgggttcggc gagcctcagc ctcgatcacg tgtccgtgag
    10141 cggcaacgag gtcagcggca gcggcggcgg catcgagaac gacggcggtg atctgacgat
    10201 ctcgcgcagc ctcctgagca acaacttcgc cgagtccgac ggcggcgcgg tcgtgagcta
    10261 cgacggcgcg accacgcaca tcgagaactc gacgattcag gacaacacgg ccgactcgaa
    10321 cgcgggctgc gccggcattg gcgccgtcac cggcttcaac gccgacatca cgctgctcaa
    10381 cgacacgatc gtcgacaacg tgaacctcga cggcacctgc agcgccgacc cgaccgcggg
    10441 cctcgcgtcc gaggacttct cgagcgacgg cgtctccgaa gagccctctg gctcgatgac
    10501 cgtgcagaac acggtcgtcg ccaacaacca gacgctcggt cccgtggagg gcgagggaac
    10561 gctcagcgtg ggcggcacgc cggagctgaa caactgcgcg ggccccggca tcaccagcgc
    10621 gggctacaac atcgaggacg acacggactg ctcgttcgcc aacaccggcg acctgcagaa
    10681 caccgatccg aagctcggtg ggctcgataa caacggcggc cagaccgaca cccgcgcgct
    10741 gctcgacggc agtccggcga tcaacaccgc cggcccgggt gctccggcca ccgaccagcg
    10801 cgatgtctcg cgcccgcagg gtgcggcctc cgacatcggc gccttcgagg tcgaggttcc
    10861 ggcaccgccc acgactcagc cggtgcagcc ggtgcagcag cagcagaagc ccggcgcgcc
    10921 gaagctggcc gtggcgggcg tgcgccgcgc ctgcacgtcc tcggctcgcg tcagcgtgaa
    10981 ggtcaaggcc accgttgcct cgggcacgac gctgaagcag gtgcgcgtca cgctcgacgg
    11041 caagcgcctc tacacgagca agaagggcac cttcacgctg aaggtgaacg ccacgaggct
    11101 gaaggccggt cgtcaccgcc tgcgtgtgac cgtgctggac tcggccggcg gcagcaagac
    11161 caccaccaag gtgctggctc gctgcgcgca ggccaagccg aaggcgaagg tgcagccgcg
    11221 cttcaccggc tgaccgctcg atagcaaagc cgaagcgcca atcccagggc gctcgcggaa
    11281 ggcactggcg cctgctgccc aaccggcagc gggcgccttt gccgttctta gcctgccgcg
    11341 gcgagcacag gcttcggccg ctcgccgccc tcccacggcc gccaggcgcg cagcgcggag
    11401 aggatcgcgc gcgtggccgg ggagcgattc agcgtgtaga agtgaatgcc cggcgcgccg
    11461 cgcgcgagca ggtcggcgga ctgcagcgtg gcgtacgcca cgcctagatc ctgcacggcg
    11521 gccgggttct cctggcgtgc tgagagctct ctttcgagct ccgccgggat ggtcgcgccg
    11581 cacatgccgg tgatgcgctt gatctgctgg tagttcgtga tcggcattat gccggggatg
    11641 atcgggacgt cgataccgaa ctcgcgcgcc ttgtccacga aattgaagta cagctcgttg
    11701 tcgtagaaga gctgcgtgat caggaagcgt gcgcccgccc ccaccttctt cttgagaaag
    11761 cgcaggtccg actccatgtc cgccgcctcc ggatgcacct ccgggaagca ggccgcgcct
    11821 accgcatact cgtagttctg cgtgatcaac tccgcgagct ccgtggagta cttcaggccg
    11881 cccggcgtgg gcttccattc ggtctccccc tgcggcgggt cgccgcgcag cgcgagcacg
    11941 ttctcgattc ccgcctcgcc gatctcgtcg aggatcgcgc gcagctcttc ccgcgtggtg
    12001 ccgacgcagc tgaggtgcga catcgcctcg accccgagat cgcgcttgat gcgcttcgtg
    12061 atctcgatgg tcctatcgcg cgtggagccc atcgctccgt acgtgaccga cacgaagccg
    12121 ggctcgtcgt gcttcagctc cgcgacggcg ttgaagaggt tctccatgcc ctcgtcggtc
    12181 ttcggcggga agaactcgaa cgagaagcac gggtagcggt cgtcgagtat ccggtcgatt
    12241 cgcatggtgt ggaagggtac gcggttggga ccgttatccc tgcggctgat cgtccggggg
    12301 cggcacgatg tcgagtccgg tcgcgacgcg ctcccgcccg tgcagccggt attgcacgtc
    12361 gacggagtcg ttctgacggt aggtgccgat cagcccgaga aatatcgatg tccagcgcca
    12421 gaagcccagg cggccttcct tcgtcagccg gcgctcgaac acgagctcac gcccctcgag
    12481 ccggtaatcg gtgccctcct gctgcggcac gccattcacg aacacgcgat agggcggttc
    12541 ggctccccgc gggagcggga cccgccacac cggcttcatt ggggcgtgac ccggcggcga
    12601 agtgcctcgt aggcgtcttc gccgtcgcgc tgctcgatca gctgccggcg gatccagccg
    12661 gtgtagctct cggtggagcc gtcgccgcgt acgtcgatac ccga
//
LOCUS       DASVXS010000065        22222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_219955, whole genome shotgun sequence.
ACCESSION   DASVXS010000065 DASVXS010000000
VERSION     DASVXS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22222
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219955"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /locus_tag="VF752_03535"
     CDS             <1..2269
                     /locus_tag="VF752_03535"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854648.1"
                     /translation="VVDYLRAARTGLGGVLPTKDDIVFERFFDETGGMQLIVHAPLGA
                     RINRALGLALRKKFCLTFDFELQAAASNDAVLLSLGPQHSFPLEDVPAFLRPHTVEDA
                     VCQAVLRSPMFIARWRWNLNRSLAVLRRKGGRVNPFNIQRMEAADLMAAVFPSLAACQ
                     DNAPAGPIEIPDHPLVRETLHDCLSEAMDIDGLKALVRRYEEGTVRLHFVDTVEPSVL
                     AHEILGGAPFTYLDEDTEIGERRSRALPLPRGLPVEPRELGRLDPDAIERVRAEATPD
                     VRDRDELHDVLLSLVVARPRPEWAEHFGALASSGRAFELHVPDRGDVLWAPTERRAEI
                     DALFPGASFVPDHPLPPGLVPVLDAEAADDAAATLLRGHLDISGPVTTEELAAATGLS
                     QSTVTISLEALRAAGFAVSGRFEPDRGEQWCARRLLARIHGYTRERRRAEVRPISQEE
                     WQRFLQSWLHAAPGTQLEGRAGLAEVIEQLQGFELPAGEWERIFAERVEAYRREWLDD
                     LCLSGEVVWGRLSVLEPAGEGDDPAPGTPDSASKTPSRRTPITFMLRQDLPWLLNAHR
                     GDVAPAEPAAGAGRDVLDALRGRGALFHSELQALTDRLPTEVEEGLWDGVARGLISAD
                     GFNAVRSLLEARTRFASRERPYRRSGSRGRRGTWRQGVEGRWTLLPAGDPIDDVDELA
                     ETVAWQLLLRWGVVFRDVYLKERLAVPWREILWALRRLEARGLIRGGRFVTGVTGEQF
                     ADELALPLLRGRRAA"
     gene            2357..2848
                     /locus_tag="VF752_03540"
     CDS             2357..2848
                     /locus_tag="VF752_03540"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX8854649.1"
                     /translation="MSAVPQGTPDLSAAYAAEHDELGPIDLVVIAYPPGAPLTGEAVP
                     LLLDLVDRGIIRVLDAMFVQKNADGSFSGFDARGLDQNSVGDLTVFEGASSGLLDDED
                     AAIAAEAIEPGTAAVLILYENRWAAPFAAAVRRNGGVVVDNHRIPHQAVIDALDRIEA
                     ASP"
     gene            2867..3163
                     /locus_tag="VF752_03545"
     CDS             2867..3163
                     /locus_tag="VF752_03545"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX8854650.1"
                     /translation="MGLIGGMARTAVVAGTATAVSNRVSRRQAERWSQQGSGYAPVQR
                     VGYAQEEPPAAAPAPAEPPARDIAAQLTDLAQLKEQGVLTEQEFAAQKARVLGT"
     gene            complement(3186..3404)
                     /locus_tag="VF752_03550"
     CDS             complement(3186..3404)
                     /locus_tag="VF752_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854651.1"
                     /translation="MASYAADIKPLFREHDRESMKSFFDLWSYDDVVENSDVILTRLA
                     AGDMPCDGAWPSEQVELFRGWVEAGAPA"
     gene            complement(3431..6016)
                     /locus_tag="VF752_03555"
     CDS             complement(3431..6016)
                     /locus_tag="VF752_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8854652.1"
                     /translation="MTKAAVKGLLGRKVRTSLTAFAVVLGVAMVSGTYVLTDTISKAF
                     DSIFNDSYKGTSAVISGKEVVTGSTSGTAPVPASLLPKVKNVNGVDAAAGAIFDVRGN
                     TDFAKLIDSNGKVISSPGGAPNFGWGIDTSQPRFNPFTLEKGKWAAGPRDVVIDSNTA
                     SKKHFNVGDTIGVSAQGPTRQFHISGVAKLGSVSSIGGATFALFTVPTAQQVLGKQGQ
                     YDEIFIAAKPGVSEQAVANEVKPLLPASATVKTARDQAKDVSSDAQSGVDFIRYLMLA
                     FAAIALVVGSFVIFNTISMTLTQRVRELATLRTLGASRKQVRRSVLLEGLIIGVVASV
                     IGLALGIALAKGLNALFVALGIDMPQQGTVVRGRTILVSLLVGVAVTVLATWGPARRA
                     TRVPPISAVREGATLPRTRLARNSTRTAAIVLGLAAGLLIYGLFAEGLATGTVLLTVG
                     LGCILLFVGVGLISSRLVGPLAAVVGAPAERFGGAPGRLARENSVRNPTRTARTAGAL
                     MIGLALVALIATLGAGVKNTDKNAVRKQVTADYVVTSSNGFESFIAGAGDAAAKAPGV
                     EVASSVRADKARALGSDIDVTGVDPATIGQVYAYGWTKGSDATLGTLGTNGAVVKKDF
                     ANDNHLAVGSNFTLTTPAGKNVPVQVKGIYEPPNKDFDPLLGSVSIPQRTFDSTFPRP
                     KNLFTFLNMSGGESSQAAAGIERALAPFPDAKVRTESSFVDSRTADFDKILSIFYVLL
                     ALSVIISLFGVVNALVLAVYERTRELGMLRAVGMTQRQTRRMVRDESVIMALIGAALG
                     LPLGIFLAAIITRALRSQSFAFSVPVLILVIMAVFAFVAGVVASMLPARRASRLNVLN
                     ALQYE"
     gene            complement(6013..6783)
                     /locus_tag="VF752_03560"
     CDS             complement(6013..6783)
                     /locus_tag="VF752_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854653.1"
                     /translation="MAIRVATGNGAGSAVASAVDAIDVTRRFGEGDSAVDALRGVSLD
                     VPEGQFTAVMGPSGSGKSTLMHILAGLDSPTSGRVSIGGEDITDMDDQELTLLRRRHI
                     GFIFQFFNLLPMLTAEENIVLPLSIAGEKPDRAWIDELTEKVGLADRRNHRPSELSGG
                     QQQRVAIARALLSRPTVLFADEPTGNLDSKTSEEVLALLRDSVDTYGQTTLMVTHDPP
                     AACTADRVLFLADGKLVRDLGRSSSHEVLEAMEEFSVR"
     gene            complement(6985..8019)
                     /locus_tag="VF752_03565"
     CDS             complement(6985..8019)
                     /locus_tag="VF752_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854654.1"
                     /translation="MADLPLPAHFDPGRVGEVWRVPYEVRALQAESWAETHGLSVASE
                     DSFRVCLLAVDVQNTFCIPEFELFVAGRSGTGAVDDNRRLCGFVYRNLGTITQIFPSL
                     DTHHAMQVFHAIWLVDEDGDHPAPYTLVSAEDVQAGRWRMNPAVAEALGIDLDYAQRH
                     LAHYTQRLAEGGKYDLTIWPYHAMLGSIGYALVSAVEEAVFFHTIARRSQPDFQVKGH
                     NPLTEHYSMLGPEVTEGPDGDALDGRTTELIEKLLTFDAVVVAGQAKSHCVAWTIDDL
                     LTDAEVRERLAPKTYLLEDCTSPVVVPGVVDYTDEANAAFERYAAAGMHVVRSTEPIA
                     SWPGLAKRVA"
     gene            8082..9152
                     /gene="ligD"
                     /locus_tag="VF752_03570"
     CDS             8082..9152
                     /gene="ligD"
                     /locus_tag="VF752_03570"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8854655.1"
                     /translation="MATTEATIIDAGGRDLRVTSPDRVIFPATERTGEITKLDIVNYY
                     VAVGDGILRALNRRPTTLERWPKGVHPGIRLSTRERGGGDAFFQKRIPKGAPDYVETA
                     RIEFPSGRTADEICPKEIADVAWAAQMGTITFHPWPVRSSDVDHPDELRLDLDPQPGT
                     DFDDAVRVAAEARVLLDDLGYVGFPKTSGGRGVHIYVRIEPRWTFTDVRHAAIAFGRE
                     LERRLPGQVTTKWWKEERGERIFVDYNQNARDRTIASAYSVRPKPGAPVSAPVTWDEL
                     PEVAPEDFTVATMPARFAEIGDRHAAIDDAAYSLQPLLDLYERDASDGLTDMPYPPDY
                     PKMPGEPKRVQPSRDRDRKKTE"
     gene            complement(9162..10022)
                     /locus_tag="VF752_03575"
     CDS             complement(9162..10022)
                     /locus_tag="VF752_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8854656.1"
                     /translation="MRIATWNVNSVKQRVPRLLPWLDERQPDVVCLQETKLADDAFVA
                     LLGDELSNRGYALALHGEATWNGVAILSRVGLEDVVPGIEGAPGFPHPESRAVSATCD
                     GIRVVSVYVPNGRTPGSEHYAYKLAWLASLREMVASGPEATIVCGDMNIAPTDDDVFD
                     PDAYIGQTHVTPPEREALAELQAVGLHDVVRDRWPNERVFTYWDYRAGMFHQDLGMRI
                     DLVLASAPVADRVKAAWVDRQARKGSGPSDHAPVIVDLDEAPDGDIGPVVPPPSRPVT
                     KRGAKKLPQA"
     gene            complement(10043..10702)
                     /locus_tag="VF752_03580"
     CDS             complement(10043..10702)
                     /locus_tag="VF752_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854657.1"
                     /translation="MSEERVRVMVVDDDDLMRAGLRGVLSSDEAIEVVGEARDGRDAL
                     YRARMLNPEVVLMDVRMPDLDGIAATRELLAAFPELKVVILTTFEQDDYIFGALSAGA
                     SGFLLKRTSPEDLVAAIHTIAAGDSLLSPSVTSRVIERMAGHPPRGDSADGRLAELTP
                     REKEVLQLVARGLSNAEIADALVIEESTVKTHVKRVLAKLALRDRVQAVIFAYENGLV
                     P"
     gene            complement(10699..11901)
                     /locus_tag="VF752_03585"
     CDS             complement(10699..11901)
                     /locus_tag="VF752_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8854658.1"
                     /translation="MATHAAFPRAPRLVRDERLSDAGIAVVVFAVSLALFAAGTHLEG
                     HRGQIDVPGVALTALASLPLAARRVAPLAVFVVTAVASAALLGIALPAGPPLGPTLAL
                     YYLSAAEQPSRGRLRLTAVVVVAMLIAHVTASGLAADRFPGPELVFGVVVWGGAWLAG
                     ERSRLRRERIAELEERALRAEREADRERRLAAAEERARIARDLHDSAGHAINVILVHA
                     GLGRLRSANEPEAARDEFETIEQVARETIGEIDKLVGALREEPAHAQRRDEVEPPPGL
                     AALDALVERHRAAGLEVTKTIRGDRRSLSPSVDRGAYRILQEALTNAARHGDGGAQLE
                     LNFEPDLLELSVVNPLRNEPGTDANGGGHGLIGMRERATLLGGSLVAGARQGRFEVRA
                     RLLTDRET"
     gene            12108..14264
                     /locus_tag="VF752_03590"
     CDS             12108..14264
                     /locus_tag="VF752_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8854659.1"
                     /translation="MSSITRWVLAHKRTVVLTWIVLTIAGFAAAGPASKALDSEFSVP
                     NKEGWETNVAIAKRYFGTGGDSSPLVPVVALPAGKSVDSPGVRADLRKVDADLRKALP
                     RARIASYASTGDRTFVSKDGRTTFALAYPTPSADSTFGENPEAEKAARAALKDTTVGG
                     APVHLTGFDALQQDSGGDGGPGVLLEALLGGAGALIVLSFVFASFLAIVPLLMAIVAI
                     PTTFLMLLGLTKVASISPIVQFLIALVGLGVAIDYSLLVVTRWREERAHGHSGDEAVQ
                     RAMETAGRAVVFSGITVAIGLLALIALPLSFLRSMGYGGMLIPLVSTLVAITLLPVIL
                     AKVGDRLDWPHRRTDNKASRTWTRWADAVARRRWVAAGFGMAIVLALVIAATDLQLGA
                     SDADTVARSGDAKQGLVALEKAGIGEGSILPHEILVDQGSDPDRVAAALRKVEGIHGA
                     VAPQGSDWRRGGTAIVEAVPVPDSGSGQGETTLDEVRDTAHASGPGVRVGGQPASNAD
                     FIDAVYGSFPLMIALITVTTFILLARAFRSLLLPLKAILLNVLSVAAAWGVMVLVWQK
                     GYGSELIWGIDATGSIPSWMPLMVFAFLFGLSMDYEVFILSRMREEYDRTGSTRTAVV
                     QGIGRTGRLVTSAALILFLAFVSMASGPGTDLKMFATGLGAGILLDATVIRALIVPAV
                     IALMGRWNWWLPEWPARLLRVEPSLPRRVAAGEADG"
     gene            14292..15149
                     /locus_tag="VF752_03595"
     CDS             14292..15149
                     /locus_tag="VF752_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HEX8854660.1"
                     /translation="MTAHDDRELVTARLARAGFVASDEEAGELVACAGGDRELLESLV
                     ERRLTGEPLAYVTGRLMFCGYEIRVDPGVYVPRWQSEALAQRAVERLPHDGAAIDICT
                     GTGAIAKTLVVEHPGARVLATDIDERAVACARANGVDAYVGDLFAPVPSELEGEVDVV
                     VGVVPYVPTPELRLLQRDTFTFESPLSYDGGADGTDILRRAITDSTRFLRRGGGLLLE
                     LGGEQADLLRDDLARLGYADARVLTDEDGDVRGIEATFAQAATSTSSVTSAPGSGDEK
                     TIAPSSIGP"
     gene            complement(15062..16588)
                     /locus_tag="VF752_03600"
     CDS             complement(15062..16588)
                     /locus_tag="VF752_03600"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8854661.1"
                     /translation="MSDATDTTGLPREHFDVLIVGAGISGIGAGYHIESRFPGKTYAI
                     LEARDDMGGTWDLFRYPGIRSDSDMHTLGFKFHPWTEAEAIADGPAILRYVHDTAREY
                     GIDRNVRFNHRVVRAEWSTPDARWTVEAERTDTGETVELTCGFLFSCSGYYRYDEGYL
                     PDFEGVERFKGVFVHPQHWPEDLDYAGKRVLVIGSGATAVTLVPAMADDAAHVTMLQR
                     SPSYIVSLPGQDPIANFLRRVLPDKTAYAVTRWKNVFIQALVYQLCRRRPDLMKRLIR
                     RGVMRQLPPGYDVDKHFTPKYNPWDQRLCLVPDGDLFKSISEGRASMVTDTIETFTEN
                     GIKLSSGEELEADIIVSATGLNLLVFGGISLSVDGLEVEVPKAMAYKSMMLSGVPNFA
                     FALGYTNASWTLKVDVTCEYVCRLLAYMDERGYRQCTPQINDPSVTEEPFLDFTAGYV
                     LRSIHKFPKQGNKQPWRLPMNYALDIRTLRYGPIEDGAMVFSSPEPGAEVTEEVEVAA
                     "
     gene            complement(16637..16930)
                     /locus_tag="VF752_03605"
     CDS             complement(16637..16930)
                     /locus_tag="VF752_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854662.1"
                     /translation="MLVAAAVVAVFAGSASAVPAVGCGKVRVGHHHYSVRAHVLACRK
                     ARSWTVSYLKGRGAPRGYECRRFSRKLTRVVFVCENPRTRSRSDGPQSFSASR"
     gene            17043..17951
                     /locus_tag="VF752_03610"
     CDS             17043..17951
                     /locus_tag="VF752_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854663.1"
                     /translation="MALKSGSPRRRPAAGLSLALLAASLLALATGCSGTALQVVDAKS
                     KRPFIDAFAVNPKGGSLLLATNKGLFHIDADGKKGRLLRSEVKVSNKGSGFGDKVSAF
                     AFVGPDKLIGSGHPDDPTVPGNLGLMQSNNGGASWKAVSRVGLSDLHVIKPQGGLVFA
                     YDTVLGGVIVSSDGGKTFAERSAPEEPVTDLASDPRDANYLLAASVNSLFVSTDQARS
                     WKKLGPADSPQLAWGEHGLYRADAGGIVGTSSDRGRSWKQVGRLEGNPGKLVETSDDK
                     LYVALDSGIVQVSSDAGRSWKRLFKP"
     gene            17948..19012
                     /locus_tag="VF752_03615"
     CDS             17948..19012
                     /locus_tag="VF752_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854664.1"
                     /translation="MSRRALAATVTLAVSGALASAADAHNDYVKVDGSTLIYQQYPGL
                     APGQPDNALSITYKTIDGDQYFQVDDPNSTGITNELPCVPHTFDDESVLCPAAGITSL
                     LIDTGTGTDQVTITAPTAARIYGGPGNDTLRGGAGATQFFGQAGNDALVAGAGGGNVL
                     DPGTGTNSLDAANGFADTVNRCTGLDTVTSDVADTLLGACPTTDVPSPLPPPDFPARP
                     TPAPHDPAGGPTPSGVLLSSLRLIPASFRAAPRGASFAVRTGARLSFRLSAAADVTFR
                     VDKLRRGRHRTFWTALGSNFKLRKRPAGITRTRFTGRVPGAVLRPGSYRLVAAPVDPD
                     GSQGGTTKVRFRIVASPKKR"
     gene            complement(19009..19470)
                     /locus_tag="VF752_03620"
     CDS             complement(19009..19470)
                     /locus_tag="VF752_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854665.1"
                     /translation="MDVLAAIRPDSWNFPLLVHLIGATTLVGALVLAGSALLFAWRDG
                     NATLAMVGYRTMLLGVLPAWIIMRGGAQWIASKEHLEDSNASWVGIGFTTADAGLAVL
                     VVATLLAWLAVRRTRREGGTPGGLGVASAFLVAVLVIAYLVAVWAMTTKPV"
     gene            complement(19472..19882)
                     /locus_tag="VF752_03625"
     CDS             complement(19472..19882)
                     /locus_tag="VF752_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854666.1"
                     /translation="MYDTLLFLHMLSAFALIATVVIFSGFVLRLRGDARILSIGNVLW
                     AIGGLGTIVFGVWLAIYVDGYDLLDGWVIAAIVLWVIASGLGGRVQAAFNEAFSAREG
                     GTAVALPSQAVTLHWLRTLAVLLLLIDMVYKPGH"
     gene            complement(19912..21705)
                     /locus_tag="VF752_03630"
     CDS             complement(19912..21705)
                     /locus_tag="VF752_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854667.1"
                     /translation="MASSGPALPPEARVPLKRIARLFRSYRGRLSVLLLLIVVAAGVG
                     VISPFLLRAVLDDAIPNKDTELLTFLVLGMIAISVLSGVIGVAQTWLSNTVGQRVMHD
                     LRSAVYSHLQRMSLAFFTRTHTGEVQSRIANDIGGIDNVVTSTATSIMSNVTTVLATL
                     VAMIALDWRLAAFSLCLLPFFVWLTRRVGKERKRITSVRQGRLADMSTLVEESLSVSG
                     ILLGKTMGRGPELAGRFRDESAELADIEVRSRMAGRWRMASVQMSFAILPAAVYWFAG
                     MNIASGGHAISIGTVVAFTTLQTRLLFPIQSLLSVGLDVQTSLAMFARIFEYLDLPVD
                     IDERRHPVELRDVRGDVTFDDVWFRYDEDAPWTLADVDAVVPAGTTTAIVGETGSGKT
                     TLAYLVARLYEPERGRVTIDGVDVAEASLSSLAATVGLVSQETYLFHASIRENLRFAR
                     PDATDAEIEQAARAAQIHDLIVSLPDGYETMVGERGYRFSGGEKQRIAIARTILRNPP
                     VLILDEATSALDNETERALQEALDELARGRTTIAIAHRLSTVRDADQILVLDHGRIVE
                     RGTHAELLELDGRYAELARGTVAGEEPSVAA"
     gene            complement(21736..>22222)
                     /locus_tag="VF752_03635"
     CDS             complement(21736..>22222)
                     /locus_tag="VF752_03635"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8854668.1"
                     /translation="RFQRAQGLRSDGIAGPATIKALKHPRRRARAHGAPRGPVRFLRP
                     VDAPIGDGFGWVSGRRHTGIDFLADAGAPVGAAGVGVVASAGWDDGGYGNLVVVQHRL
                     HWQTWYAHLSSITVAQGQAVSGGTLIGYVGSTGRATGPHLHFETRHRGVPVDPMPRLL
                     P"
BASE COUNT         3502 a         7769 c         7598 g         3353 t
ORIGIN      
        1 tgtcgtcgac tacctccgcg ccgcgcggac cgggctcggc ggagtgctac ccacgaagga
       61 cgacatcgtg ttcgagcgct tcttcgacga gaccggcggg atgcagctca tcgtccacgc
      121 gccgctcggt gcgcgaatca accgcgcact cggccttgcg ctgcggaaga agttctgcct
      181 gaccttcgac ttcgagcttc aagcggcggc cagcaacgac gccgtactcc tgtcgctcgg
      241 cccgcagcac tcgttcccgc tcgaggacgt gcccgccttc cttcgcccgc acaccgtcga
      301 ggacgccgtc tgccaggccg tgctgcgcag cccgatgttc atcgcccgct ggcgctggaa
      361 cctgaaccgg tcgctcgcag tgctgcgacg aaagggcggc cgcgtcaacc cgttcaacat
      421 ccagcgcatg gaagcggccg acctgatggc ggccgtcttc ccctcgctcg ccgcctgcca
      481 ggacaacgcc ccggccggtc cgatcgaaat cccggatcac ccgctcgtgc gcgagacgct
      541 gcatgactgc ctgagcgagg ccatggacat cgacggccta aaggcactgg tccgccgcta
      601 cgaggaaggg accgttcggc tccacttcgt cgacacagtc gagccaagcg tgctggctca
      661 cgagatcctt ggcggcgcgc cgttcactta cctcgacgag gacaccgaga tcggcgagcg
      721 ccgatcgcgc gccctaccgc tgccgcgtgg cctgcccgtc gagcctcgcg agctcggaag
      781 gctagacccc gacgccatcg agcgggtgcg ggccgaggcg actcccgacg tgcgcgaccg
      841 cgacgagctt cacgacgtgc tcctctcgct cgtagtcgcc cggccgcgac ccgagtgggc
      901 cgagcatttc ggggctcttg cgagctctgg ccgcgccttc gagcttcacg ttcccgaccg
      961 aggcgatgtc ctgtgggcgc cgaccgagcg acgcgccgag atcgatgcgc tcttccccgg
     1021 ggcgagcttc gtaccggatc acccgcttcc gcctgggctg gtccccgtcc ttgacgcaga
     1081 agcggccgac gacgcggcag cgactctcct tcgcggtcac ctcgacatct ctggtccagt
     1141 taccaccgaa gagctcgccg cggccacggg tctctcacag tcaacagtca cgatcagcct
     1201 cgaggcgctc cgcgcggccg ggttcgcggt gagcggccgg ttcgagccgg atcggggcga
     1261 gcagtggtgc gcccgccggt tgctcgcccg catccacggc tacacgcgcg agcgccggcg
     1321 cgccgaggtc cgcccgatca gccaggaaga gtggcagcgg ttcctccagt cctggctgca
     1381 cgccgctccc ggcacccagc tcgagggccg ggccggcctt gctgaagtga tcgagcagct
     1441 ccagggcttc gagctcccgg cgggcgaatg ggagcggatc ttcgccgagc gcgtcgaggc
     1501 ctaccgccgc gagtggctcg acgatctctg tctgtccggc gaggtggtgt ggggccgcct
     1561 gtccgtgctc gagcccgccg gggagggcga cgatcccgcg cccgggacac ccgacagcgc
     1621 gagcaagacg ccgtcgcgcc ggacgcccat caccttcatg ctccggcagg acctgccttg
     1681 gctcctcaac gcccatcgcg gcgacgtcgc gccggccgag cctgccgccg gcgccgggcg
     1741 ggacgtgctc gacgcgctac gcgggcgcgg cgccctgttc cactctgagc tccaggcgct
     1801 gaccgatcgc ctccccactg aggtcgagga aggcctgtgg gacggcgtcg cgcgcgggct
     1861 catcagcgca gatggattca acgccgtccg gtcacttctc gaggcgcgca cccggttcgc
     1921 gagccgcgag cggccctacc ggcgttcggg ttctcgcggc cggcgcggca cctggcgaca
     1981 gggtgtggag ggccgctgga cgctgctgcc cgccggcgat ccgatcgacg acgtcgacga
     2041 gctggccgag accgttgcct ggcagctcct gctgcgctgg ggcgtggtct tccgcgacgt
     2101 gtatctcaag gaacggctcg ccgttccttg gcgggagatc ctctgggccc tgcgccgcct
     2161 cgaggcccgc ggcctgatcc ggggcgggcg cttcgtgacc ggtgtgacgg gcgagcagtt
     2221 cgccgacgag ctggcgttgc cgctgttgcg cggccgccgc gccgcctgag ctccgcggac
     2281 attcatccgg ttcgggcgat gcgagcagtt gcctcggcgc tcaatgatcg cgtttacctg
     2341 atcaggagga cgccacatga gcgcagtgcc acagggcacc ccagacctct ccgcggccta
     2401 cgccgcggag catgacgagc tcggccccat cgacctcgtg gtgatcgctt atccgcccgg
     2461 tgcaccgctc accggcgagg cggtaccgct cctcctcgat ctcgtcgatc gcggaatcat
     2521 ccgcgtgctg gacgcgatgt tcgttcagaa gaatgcggac ggaagcttct ccggcttcga
     2581 cgcgcgagga ctcgaccaga actcggttgg cgatctcacg gtgttcgagg gcgcgtcctc
     2641 cgggctcctg gatgacgagg acgctgcaat agccgccgaa gcgatcgagc cggggacggc
     2701 ggccgtcctg atcctctatg agaaccgctg ggccgcgcca tttgcggccg cggtgcgacg
     2761 caacgggggc gtcgtggtgg acaaccaccg aattccgcac caagccgtga tcgacgccct
     2821 ggaccggatc gaagcggcgt cgccgtgagt cgaagggaga gctgaaatgg gcctcatagg
     2881 agggatggcg cgtaccgcgg tagtcgcggg gacggccacg gccgtttcaa accgcgtgag
     2941 ccggcgtcag gccgaacgct ggtcgcagca gggctcgggc tatgcgcccg tgcaacgcgt
     3001 gggctacgcc caggaggagc cgcccgcagc ggcgccggct ccagccgagc cgcccgcacg
     3061 tgacatcgcg gctcagctca cggacctcgc acagctgaag gagcagggcg tgctgaccga
     3121 gcaggaattc gccgcccaga aggcgcgcgt cctcggaacc tgacgaccaa acctgacgac
     3181 cacggttacg caggggcgcc cgcctctacc cagcccctga agagctctac ttgctccgag
     3241 ggccaggcac cgtcgcaggg catgtcgccc gccgcgaggc gagtgaggat cacgtcgctg
     3301 ttctcgacga cgtcgtcata ggaccagagg tcgaagaaac tcttcatcga ctcgcggtcg
     3361 tgttcccgga agagcggctt gatatcggct gcgtagctcg ccatgaggcg tcaatcaggc
     3421 gtgtgcatgg ctactcgtac tgcagcgcgt tcagcacgtt gaggcgcgac gccctcctgg
     3481 ccggcagcat cgaggcgaca acgccggcca cgaacgcaaa caccgccatg atcaccagga
     3541 tcagcaccgg cactgagaac gcgaacgact gcgagcgcag cgcccgcgtg atgatcgccg
     3601 ccaggaagat cccgagcggc aggccgagcg ccgcgccgat cagcgccatg atcacgctct
     3661 cgtccctcac catgcggcgg gtctgccgct gcgtcatgcc aaccgcccgc agcatcccga
     3721 gctcccgcgt gcgctcgtac acggccagca ccagcgcgtt caccacaccg aacaggctga
     3781 tgatcaccga cagcgcaagc agcacgtaga agatcgacag gatcttgtcg aagtcggcgg
     3841 tgcggctgtc gacgaagctc gactccgtgc gcaccttggc gtcggggaac ggcgccagcg
     3901 cgcgctcgat ccccgctgcc gcctgtgagc tctcgccgcc actcatattc aggaaggtga
     3961 ataggttctt cgggcgcggg aatgtcgagt cgaaggtccg ctgggggatg ctcaccgacc
     4021 cgagaagtgg gtcgaagtcc ttgttcggcg gctcgtagat gcccttcacc tgcaccggca
     4081 cgttcttgcc cgccggtgtg gtgagcgtga agttactgcc cacggcgagg tggttgtcgt
     4141 tggcgaagtc cttcttcacg actgcgccgt tcgtcccgag cgtgccgagc gtcgcatctg
     4201 acccctttgt ccagccatac gcgtagacct ggccgatcgt tgccgggtcg actcccgtca
     4261 catcgatgtc cgagccgagc gcacgcgcct tgtcggctct cacactcgac gcgacctcga
     4321 cgccaggcgc cttggcggcg gcgtcgccgg cgcccgcgat gaagctctcg aagccgttgc
     4381 tcgaggtgac cacgtagtcg gccgtcacct gcttcctgac ggcgttcttg tcggtgttct
     4441 tgacgcctgc gccgagcgtc gctatcagcg cgacgagcgc gagcccgatc atcaatgcgc
     4501 cggcggtcct cgcagtccgc gtcggattgc gcacggagtt ctcgcgcgcg agccgccccg
     4561 gtgcgccgcc gaaccgctcc gccggcgcgc cgacgaccgc ggccagcggt cccacgagcc
     4621 gggaagagat cagccccaca cccacgaaga gcaggatgca gccaaggccg acggtgagca
     4681 gaacggtccc ggttgcgagg ccctcggcga agagcccgta gatgagcagc cccgccgcga
     4741 gaccgagcac gattgcggcg gtcctcgtgc tgttgcgggc gagccgcgta cggggcaggg
     4801 ttgcgccctc gcgcaccgcc gatatcggcg gcacgcgcgt cgcccgtcgc gcggggcccc
     4861 aggtcgcgag cacggtcacg gcgacgccga cgagcagcga gacgaggatc gtcctgccgc
     4921 ggacgaccgt tccctgctgc ggcatgtcga tccccagggc gacgaagagt gcgttcagtc
     4981 ccttcgcaag cgcgatcccg agcgccagcc cgatcaccga ggcaaccacg ccgatgatga
     5041 gcccttcgag cagcacagac cgcctgacct gcttccgcga ggcaccgagc gtccgcagcg
     5101 tcgcgagctc gcgcacgcgc tgagtgaggg tcatcgagat cgtgttgaag atgacgaacg
     5161 agcccaccac cagcgcgatc gcggcaaacg ccagcatgag gtagcggatg aagtcgacgc
     5221 cgctctgagc gtcgctcgag acgtccttcg cctggtcgcg cgccgtcttc accgtcgccg
     5281 aggcgggcaa cagaggcttc acctcgttgg caaccgcctg ctcggacacg cccggcttcg
     5341 ccgcgatgaa gatctcgtcg tactgaccct gcttgcccag cacctgctgt gcggtgggca
     5401 cggtgaagag ggcgaaggtg gcgccgccga tcgagctcac cgagccgagc ttcgccacac
     5461 cggagatgtg gaactgcctc gtcggcccct gcgcggacac gccgatcgtg tcaccaacgt
     5521 tgaagtgctt cttgctcgcg gtgttggagt cgatcaccac gtctcggggt ccggccgccc
     5581 actttccctt ctcgagcgtg aacgggttga agcgcggctg gctcgtatcg atgccccagc
     5641 cgaagttcgg cgcgccgccc gggctggaga tcaccttgcc gttcgaatcg atcagcttcg
     5701 cgaagtcggt gttgccacgg acgtcgaaga tcgctccggc ggccgcgtcc acgccgttca
     5761 cgttcttgac cttgggcagc aaggaggccg gtacgggtgc agtgccactt gtcgacccgg
     5821 tcacgacttc cttgccgctg atgacggcgc tcgtgccctt gtacgagtcg ttgaagatcg
     5881 agtcgaacgc cttcgagatc gtgtcagtga ggacgtaggt gccgctgacc atcgccacac
     5941 cgagcacgac ggcaaacgcg gtcaggctcg tgcggacctt ccgcccgagt agccctttta
     6001 cggcggcctt ggtcatcgca cgctgaactc ctccatcgct tcgagcacct cgtgcgagct
     6061 cgagcggccg agatcacgga ccagcttgcc gtcggcgagg aacagcaccc gatcggccgt
     6121 acaagctgcc ggcgggtcgt gggtcaccat cagcgtggtc tgaccgtagg tgtccaccga
     6181 gtcgcgcagg agcgcgagca cttcttcgct cgttttcgag tcgaggttgc cggtcggctc
     6241 gtcagcgaac agcaccgtcg ggcgcgaaag gagtgcacgc gcgatcgcga cgcgctgctg
     6301 ctgtccgcct gacagctccg acggccggtg gttgcggcga tccgcgagcc cgaccttctc
     6361 ggtgagctcg tcgatccaag cgcggtccgg cttctcgccc gcaatcgaca gcgggagaac
     6421 gatgttctcc tcggccgtca gcatcggcag gaggttgaag aactggaaga tgaagccgat
     6481 gtggcggcgg cggagcagag tcagctcctg gtcgtccatg tcggtgatgt cctcgccgcc
     6541 gatcgacact cgcccgctgg tcggcgagtc gaggccggcg aggatgtgca tcagcgtgga
     6601 cttgcccgag cccgacggtc ccatgaccgc ggtgaactgc ccctcgggca catcgagcga
     6661 gacgcctcga agcgcgtcga ccgccgagtc gccctcgccg aagcggcgcg tgacgtcaat
     6721 cgcatcgacg gcactggcga cggccgatcc ggcaccgttt ccggtggcga cgcggatggc
     6781 catgattgcc tccttcaggc cccgtcggac cgctggcgct acgagcctca ggttgccgag
     6841 tcgtggaggc cggtctgtcg ctgcgcacct accgtgaagc gccggcggga ccagatcgcc
     6901 gggccgggct tgcgcggacg ctcggggcga agcggccgat caaccctttc ttgtgcgcga
     6961 cgctaaaccg ttcaagaaag cgggtcaagc gacccgcttc gcgagcccgg gccagctcgc
     7021 gatgggctct gtcgagcgca ccacgtgcat gcccgcggcg gcgtaacgct caaaggcggc
     7081 gttcgcctca tcggtgtagt cgacgacgcc gggcacgacc accggcgacg tgcagtcctc
     7141 gaggaggtac gtctttggcg caagtcgctc gcgcacctcg gcatccgtaa gcaggtcgtc
     7201 gatagtccag gcgacgcagt ggctcttcgc ctgcccggcg acgacgacgg cgtcgaaggt
     7261 cagcagcttc tcgatgagct cggtagtccg tccgtccagg gcatcgccgt ccggtccctc
     7321 ggtgacctcg gggccgagca tcgagtaatg ctcggtgagc gggttgtggc ccttcacctg
     7381 gaagtcgggc tggctgcgcc tggcaatcgt gtggaagaag accgcctcct cgactgcgga
     7441 cacgagcgcg tacccgatgc tgccgagcat cgcgtggtag ggccagatgg tgaggtcgta
     7501 cttcccgccc tccgccagcc gctgcgtgta gtgggcgagg tggcgctgcg cgtagtcgag
     7561 gtcgattccg agcgcctcgg ccaccgccgg gttcatcctc cagcggccgg cttggacgtc
     7621 ctcggccgag acgagcgtgt agggcgcggg gtggtctccg tcctcgtcga cgagccagat
     7681 cgcgtggaac acctgcattg cgtggtgcgt gtccaggctc gggaagatct gggtgatggt
     7741 gccgaggttg cggtagacga acccgcagag gcggcggttg tcgtcgacgg cgccggtgcc
     7801 cgaacgcccg gccacgaaca actcgaactc cgggatgcag aacgtgttct gaacgtcgac
     7861 cgcgagaagg cagacgcgga aggagtcttc agacgccacg ctcagcccgt gagtctccgc
     7921 ccacgactcc gcctggagcg cccggacttc gtatggaacg cgccacacct ctcccacgcg
     7981 gcccgggtcg aagtgcgcag gcagcggtag atcagccatt acgcacggga gtaaaccagc
     8041 ctcgccaagg gcgaaagggt tcccctgtag aactagtggg catggccacc accgaagcga
     8101 cgatcatcga cgccggcggg cgcgatctgc gcgtaacgag ccccgaccgt gtgatcttcc
     8161 cggcgaccga gcgcaccggc gagatcacca agctcgacat cgtcaactac tacgtcgccg
     8221 tgggcgacgg catcttgcga gcgctcaacc gccggcccac aaccctcgag cgctggccga
     8281 agggcgtgca cccgggcatc cgcctctcga cccgcgaacg cggcggcggc gacgccttct
     8341 tccagaagcg catcccgaag ggcgcgcccg actacgtgga gacggcgcgg atcgagttcc
     8401 cttcgggccg caccgcggat gagatctgcc ccaaggagat cgcggacgtc gcgtgggccg
     8461 cgcagatggg cacgatcacg ttccatccct ggccggttcg cagcagcgac gtcgatcatc
     8521 cggatgagct ccgcctcgac ctcgaccccc agccgggcac cgacttcgac gacgccgtgc
     8581 gcgtcgccgc tgaggcgcgc gtcctgctcg acgacctcgg atacgtgggc ttcccgaaga
     8641 cgtcgggcgg ccgcggcgtc cacatctacg tccgcatcga gccgcgctgg acgttcacgg
     8701 acgtgcggca cgccgcgatc gcattcggtc gtgagctcga gcgtcgcctg ccgggccagg
     8761 tgaccacgaa gtggtggaag gaggagcgcg gcgagcgcat cttcgtcgac tacaaccaga
     8821 acgcgcgcga ccgcacgatc gcctcggcgt acagcgtgcg gccgaagccg ggtgcgccgg
     8881 tctcggcgcc tgtcacatgg gacgagctcc cggaggtcgc gcccgaggac ttcaccgtcg
     8941 ccacgatgcc ggcccgcttc gccgagatcg gcgaccgcca cgcggccatc gacgacgccg
     9001 cgtactcgct gcagccgctg ctcgacctgt acgagcgcga cgcctccgac ggcctgaccg
     9061 acatgccgta cccgcccgac tacccgaaga tgccgggcga gccaaagcgc gtgcagccgt
     9121 cgcgcgaccg ggaccggaag aagaccgagt agcgcgcgcg gctacgcctg cggcagcttc
     9181 ttcgcgccgc gcttcgtgac cggccgcgac ggcggcggca cgacggggcc gatgtcgccg
     9241 tccggcgcct cgtcgaggtc gacgatcacg ggcgcgtggt cgctcggacc gctgcccttg
     9301 cgggcctggc ggtccaccca cgccgccttc acccgatcgg ccaccggcgc gctcgcgagc
     9361 acgaggtcga tccgcatgcc gaggtcctgg tggaacattc cggcgcggta gtcccagtac
     9421 gtgaacacgc gctcgttcgg ccagcgatcg cgcaccacgt cgtgcagccc caccgcctgc
     9481 agctccgcga gcgcctcccg ctcgggcggc gtcacgtgtg tctggccgat gtacgcgtcg
     9541 gggtcgaaca cgtcgtcgtc cgtgggcgcg atgttcatgt cgccgcagac gatcgtcgcc
     9601 tcggggccgg aggcgaccat ctcgcgaagc gacgccaacc aggcgagctt gtaggcatag
     9661 tgctcggagc caggcgtgcg gccgttaggc acgtacaccg acaccacgcg gatgccgtca
     9721 caggtggctg acacagcgcg cgactccgga tgcgggaagc ccggcgcgcc ctcgataccc
     9781 ggcacgacgt cctccaggcc gacccgcgag aggatcgcga cgccgttcca ggtcgcctcg
     9841 ccgtggagcg ccagcgcgta gccacggttc gagagctcgt cgcccagcag cgccacgaac
     9901 gcgtcgtccg ccagcttggt ctcctgcagg cacaccacgt ccggctggcg ctcgtccagc
     9961 cacggcagca gccgcggcac gcgctgcttc accgagttga cgttccaggt cgcgattcgc
    10021 acggaccgga aggtagcgag ggctacggga ccaggccgtt ctcgtaggcg aagatcacgg
    10081 cttgcacgcg gtcgcgcagg gcgagcttcg caagcacccg cttcacgtgc gtcttgacgg
    10141 tcgactcctc gatcaccagc gcgtcggcga tctcggcgtt ggacagaccg cgcgccacca
    10201 gctggagcac ctctttctcg cgcggagtta gctcggcaag acgcccgtcg gcagagtccc
    10261 cgcgcggcgg gtgcccggcc atgcgctcga tcacccgact cgtgaccgag ggcgagagca
    10321 gagagtcgcc ggcggcgatc gtgtggatcg cggccacgag atcctccggc gaggtgcgct
    10381 tgagcaggaa ccccgaggcg cctgcgctga gcgcgccgaa gatgtagtcg tcctgctcga
    10441 aggtcgtgag gatcaccacc ttcagctccg ggaacgccgc gagcagctcg cgcgtcgccg
    10501 cgatgccgtc gagatcgggc atgcgcacgt ccatgagaac gacctcgggg ttcagcatgc
    10561 gtgcgcggta gagcgcatcg cgcccgtcgc gagcctcgcc gacgacctcg atcgcctcgt
    10621 cgctcgagag cacgccgcga agcccagccc gcatgagatc gtcgtcgtcg acgaccatga
    10681 cccggacgcg ctcttcgctc atgtctcccg gtcggtcaga agccgcgccc tgacctcgaa
    10741 ccggccctgt cgcgcgcccg ctacgagact gcccccgagc agcgtcgccc gctcacgcat
    10801 tccgatgagc ccgtgtccac cgccgttcgc gtcggtgccc ggctcgttgc gcagcgggtt
    10861 gaccacgctg agctcgagaa ggtccggctc gaagttgagc tcgagctgcg cgccgccgtc
    10921 gccatgcctc gcggcgttgg tgagcgcctc ctggaggatg cggtacgcgc cgcggtccac
    10981 ggacggcgaa agcgaccgcc ggtcaccgcg gatcgttttt gtcacctcta ggccggcagc
    11041 gcgatggcgc tcgacgagcg catccagcgc ggccaggccc ggcggcggct cgacctcgtc
    11101 gcgacgctgt gcgtgcgccg gctcctcacg cagggcaccc acgagcttgt cgatctcccc
    11161 gatggtctcg cgcgccacct gctcgatggt ctcgaattcg tcgcgcgcgg cctcgggctc
    11221 gttggcggag cgaaggcgcc caaggcccgc atgcacaagg atcacgttga tggcgtggcc
    11281 cgccgagtca tgaaggtcgc gggcgatgcg tgcccgctcc tcggccgccg ccagccgtcg
    11341 ctctcgatcg gcttcgcgct cggcgcgcag cgcccgctcc tcgagctcgg cgatccgctc
    11401 gcgccgcagc cgtgaccgct caccagcgag ccaggcgccg ccccacacga ccacgccgaa
    11461 cacgagctcg ggccccggga agcggtcggc cgcgagcccg ctggcagtca catgcgcgat
    11521 cagcatcgcg acgacgacga ctgcggtgag acgcaggcga cctcgcgacg gctgctcggc
    11581 cgcgctcagg tagtagagcg cgagcgtggg cccgagcggc gggccggcag gcagcgcgat
    11641 ccccaacagc gccgcgcttg cgaccgccgt gaccacgaag accgctaagg gcgctacgcg
    11701 tcgcgccgcc agcggcagcg aggcgagcgc cgtgagtgcg acgccgggca cgtcgatctg
    11761 gccgcgatgc ccttcgaggt gcgtcccggc ggcgaacagc gcaagcgaca cggcgaacac
    11821 cacgacggcg atccccgcgt cggagaggcg ctcgtcgcgc acgagccgtg gagcccgagg
    11881 gaatgccgcg tgtgttgcca tccgagtcga gcgtagcggt cagtgcgtgc accgaaatcg
    11941 ctcgccgggt ggacgtcgca tccacccgtg gtggtacgcg gaatcccacc gccgggcgtt
    12001 tccgatccgc cgtcgcttcc ctacctttcc gggacccggc gatgagtttc gacactcttc
    12061 ccggtctcaa gacggacgga tcagacgacc accctggaga taacgctttg tcctctatca
    12121 cccgctgggt cctcgcccac aaacgcaccg tagtcctcac ctggatcgtg ctcacgatcg
    12181 ccggcttcgc ggcggctgga cccgcctcga aggccctcga ctccgaattc tccgtgccga
    12241 acaaggaggg ctgggagacg aacgtggcga tcgccaagcg ctacttcgga accggaggcg
    12301 acagctcgcc gctcgtgccg gtcgtggcgc tccctgcggg taagtcagtc gattcgccgg
    12361 gtgttcgcgc cgacttgagg aaggtcgacg ccgacctgcg caaggcgctg ccgcgcgctc
    12421 gcatcgcctc ctacgcgtcg accggggaca ggacattcgt gtcgaaggac ggccgaacca
    12481 ccttcgcgct cgcgtacccg acgccgagtg cggactccac cttcggcgag aaccccgagg
    12541 ccgagaaggc ggcgcgcgcc gcgctcaagg acacaaccgt gggcggagca ccggtgcacc
    12601 tgaccgggtt cgacgcgctt cagcaggact ccggcggcga cggcgggccg ggcgtgctgc
    12661 tcgaggcgct gctgggcggg gcgggcgcgc tgatcgtgct cagcttcgtg ttcgcgtcgt
    12721 tcctagcgat cgttccgctg ctgatggcga tagtcgcaat ccctacgacg ttcttgatgc
    12781 tgctcgggct gacgaaggtg gcgagcatct cgccgatcgt gcagttcctg atcgcgctcg
    12841 tgggactcgg cgtggcgatc gactactcgc tgctcgtggt cacccgctgg cgcgaggagc
    12901 gcgcgcacgg gcactccggc gacgaggccg tccagcgcgc gatggagacc gccgggcgcg
    12961 cggtcgtgtt cagcgggatc acggtcgcga tcgggctcct cgcgctgatc gcgctgccgc
    13021 tgtcgttcct ccgctcgatg ggctacggcg ggatgctcat cccgctcgtc tcgacgctcg
    13081 tcgcgatcac gctgctgccg gtgatcttgg ccaaggttgg cgaccggctc gattggccgc
    13141 accgtcgcac cgacaacaag gcgagccgca cgtggacccg ctgggccgac gccgtcgcgc
    13201 ggcggagatg ggtggccgcc ggcttcggca tggcgatcgt gctggcgctc gtaatcgcgg
    13261 ccaccgatct gcagctcggc gcgtccgacg ccgacaccgt cgcgcggtcc ggcgatgcca
    13321 agcagggcct cgtcgctctc gagaaggcag ggatcggcga aggctcgatc cttccccacg
    13381 agatcctcgt cgatcagggc agcgaccccg accgtgtggc agcagcgctc cgcaaggtgg
    13441 aaggcatcca tggcgctgtc gctccgcagg gatccgactg gcgccgcggc ggaacggcga
    13501 tcgtggaggc ggtccccgta cccgacagcg gttcaggtca gggagagacg acgctcgacg
    13561 aggtccgcga caccgcgcac gccagtggac ccggcgtgcg cgtcggcggt cagccggcct
    13621 cgaatgccga cttcatcgac gccgtctacg gcagcttccc gctgatgatc gcgctgatca
    13681 cggtgacgac gttcatcctg ctcgcgcgcg ccttccggtc gctcctgctg ccgctcaagg
    13741 cgatcctgct gaacgtgttg agcgtcgccg cggcctgggg cgtaatggtc ctcgtctggc
    13801 agaagggcta cggctccgag ctgatctggg ggatcgacgc gacgggctcg atcccgtcgt
    13861 ggatgccgct gatggtgttc gccttcctgt tcggcctctc gatggactac gaggtgttca
    13921 tcctgtcccg catgcgggag gagtacgacc gcacgggctc gacccgcacc gcggtggtcc
    13981 agggcatcgg acggactggc cggctggtca cgagcgcagc tctgatcctg ttcctcgcgt
    14041 tcgtctcgat ggcgtccggc ccaggtaccg acctgaagat gttcgccacg gggctcggcg
    14101 ccgggatcct gctcgacgcc accgtgatcc gcgcgctgat cgtgcccgcg gtgatcgcgc
    14161 tgatgggccg ctggaattgg tggctccccg aatggccggc gcgcctgctg cgggtggagc
    14221 cctcgctgcc ccgccgcgtg gccgccggcg aggcggatgg atgaggtgcc gtgcggcgat
    14281 actgagccgg catgacggca cacgacgacc gtgagctggt cacggcccgg ctcgcacggg
    14341 ccggtttcgt cgcgtccgac gaggaggcgg gcgagctcgt cgcgtgcgcg ggcggcgacc
    14401 gcgagctgct cgagtccctc gtcgagcgcc gcctcaccgg tgagccgctc gcgtacgtca
    14461 ccggacgtct gatgttctgc ggctacgaaa tccgcgtaga cccgggcgtc tacgtgccgc
    14521 gctggcagag cgaggcgctg gcgcagcgag ccgtggagcg cctgccgcac gacggtgcag
    14581 cgatcgatat atgcaccggc acgggcgcga tcgccaagac attggtcgta gagcatccag
    14641 gcgcacgcgt gctcgcgacc gacatcgacg aacgggcggt cgcgtgtgcg agggcgaatg
    14701 gggtcgacgc gtacgtcggc gatctcttcg cgcccgtgcc gagcgagctc gagggggagg
    14761 tggacgtggt ggtcggcgtc gtgccctacg tcccgacgcc cgagctgcgc ctgctgcagc
    14821 gcgacacgtt cacgttcgag tcgccgctct cctacgacgg cggcgctgac ggcaccgaca
    14881 tcctccggcg cgccatcacc gacagcactc gctttctacg ccgcggcggc ggcctgctgc
    14941 tggaactggg aggcgagcag gccgacctgc tgcgcgacga cctggctcga ctcggctacg
    15001 ccgacgcccg cgtgctcacc gacgaagacg gcgacgtccg tggcatcgaa gccacgttcg
    15061 ctcaggccgc tacctcgacc tcctcggtca cttccgcgcc gggctcgggc gacgagaaga
    15121 ccatcgcgcc gtcctcgatc ggtccgtagc gcagcgtcct gatgtcgagc gcgtagttca
    15181 tcggcagccg ccacggctgc ttgttgccct gcttcgggaa cttgtggatc gagcgcagca
    15241 cgtagcccgc cgtgaagtcg aggaacggct cttcggtgac ggacgggtcg ttgatctgcg
    15301 gcgtgcactg gcggtagccg cgctcgtcca tgtaggcgag cagacggcac acgtactcgc
    15361 atgtcacgtc gaccttcagc gtccaggagg cgttggtgta accaagcgcg aacgcgaaat
    15421 tcggtacgcc gctcagcatc atgctcttgt aagccatcgc cttcgggacc tcgacctcga
    15481 ggccgtcgac cgacaggctg ataccgccga agacgagcag gttgaggccg gtcgccgaca
    15541 cgatgatgtc cgcctcgagc tcttcgcccg agctcagctt gatcccgttc tcggtgaagg
    15601 tctcgatcgt gtcggtgacc atcgacgcgc gcccctcgct gatcgacttg aacaggtcgc
    15661 cgtcgggaac gaggcacagg cgctggtccc aggggttgta cttgggcgtg aagtgcttat
    15721 cgacgtcgta gccgggcggc agctggcgca tcacgcctcg gcggatcagg cgcttcatca
    15781 ggtccggccg gcggcggcag agctggtaca cgagcgcctg gatgaagacg ttcttccagc
    15841 gggtcaccgc gtacgcggtc ttgtcgggta gcacacggcg taggaagttc gcgatcgggt
    15901 cctgccccgg cagcgagacg atgtagctcg gcgagcgctg aagcatcgtc acgtgagcgg
    15961 cgtcgtcggc catcgccggc acgagcgtga ccgctgtggc gccgctgccg atcacgagga
    16021 ctcgcttgcc cgcgtaatcg aggtcctcgg gccagtgctg cggatgcacg aacacgccct
    16081 tgaagcgctc gactccctcg aagtcgggca ggtagccctc gtcgtagcgg tagtagccgc
    16141 tgcacgagaa caggaagccg caggtgagct ccaccgtctc accggtgtcc gtgcgctcgg
    16201 cctcgaccgt ccagcgcgcg tccggtgtag accactccgc gcgcacgacg cggtggttga
    16261 agcgcacgtt gcgatcgatg ccgtactcgc gcgcggtgtc gtgcacgtag cgcaggatcg
    16321 ccgggccgtc ggcgatcgcc tcagcctcgg tccacgggtg gaacttgaac ccgagcgtgt
    16381 gcatgtcgga gtcggagcgg attccggggt agcggaagag atcccaggtg ccgcccatgt
    16441 cgtcgcgcgc ctcgaggatc gcgtacgtct tgccggggaa cctcgactcg atgtggtaac
    16501 ccgcgccgat gccggagatt ccggcgccaa cgatcagcac atcaaagtgt tcgcgtggca
    16561 acccggtcgt gtcagtggcg tccgacattt gtgtactgac gcgtacctta ctgtttcgcg
    16621 gccgcgcgtg cagcgcctag cgcgatgcgc tgaagctctg cggtccgtcc gagcggctgc
    16681 gcgtcctcgg gttctcgcac acaaagacga cacgcgtgag cttccgcgag aagcgccggc
    16741 attcgtaccc gcgcggcgcg ccgcgcccct tgaggtacga aaccgtccac gagcgcgcct
    16801 tccggcaggc gagcacgtga gcacggacgg aatagtgatg gtgcccgacc ctcaccttgc
    16861 cgcagccgac cgccggaacc gccgaggccg accccgcaaa caccgccacc acagcagccg
    16921 caacgagcac gaggcatcca acgagcgcaa gcgctcgacg gggcctggcc gagcttgagg
    16981 gggccgatct caatcgcgat ccaattcgcg tgcgagccga agcgatcgta tctttggtcg
    17041 cagtggcgct caaatctggc tcaccgcgcc gccggcccgc cgccgggctg tcactcgcac
    17101 tcctggcggc ctcgctcctc gcccttgcga ccggctgcag cggcactgcg ctccaggtgg
    17161 tcgacgcgaa gtcgaagcgg ccgttcatcg acgcgttcgc cgtgaacccg aagggcggat
    17221 ctctcctgct cgccacgaac aaaggcctat tccacatcga cgccgacggc aagaaggggc
    17281 gccttctgcg cagcgaggtg aaggtgtcga acaagggcag tggcttcggc gacaaggtgt
    17341 ccgcgttcgc tttcgtggga ccggacaagc tgatcggctc gggccacccc gatgacccga
    17401 ccgtgcctgg caatctcggg ctgatgcagt cgaacaacgg cggcgcgagc tggaaggcag
    17461 tctcgcgggt cggtctctcg gatctgcatg tgatcaagcc gcagggcggt ctcgtgttcg
    17521 cctacgacac ggtgctcggc ggggtgatcg tgagtagcga cggcggcaag accttcgccg
    17581 agcgcagcgc accggaggag ccggtgaccg acctggcgtc cgacccgcgc gacgcgaact
    17641 acctgctagc cgcgagcgtg aactcgctct tcgtctcgac cgaccaggct cgctcttgga
    17701 agaagctggg gccggcggat tcgccccagc tcgcgtgggg cgagcacggc ctgtaccgcg
    17761 cggacgctgg aggaatcgtt gggacgagca gcgatcgcgg gcgcagctgg aagcaggtcg
    17821 ggcggctcga gggcaacccc ggcaagctgg tcgagacgtc cgacgacaag ctctacgtgg
    17881 cgctcgacag cggcatcgtc caggtctcga gcgacgccgg ccgcagctgg aagaggctgt
    17941 tcaagccgtg agccgccggg cgctcgcggc gacggtcacg ctcgctgtgt cgggcgcgct
    18001 tgcctccgcg gctgatgcgc acaacgacta cgtgaaggtc gacggctcca cgctgatcta
    18061 ccagcagtac cccgggctcg cgccgggcca gcccgacaac gccctctcga tcacctacaa
    18121 gacgatcgac ggcgaccagt acttccaggt cgacgacccc aacagcacgg gcatcacgaa
    18181 cgagctcccg tgcgtgccgc acacattcga cgacgaaagc gtgctctgcc ctgccgcggg
    18241 gatcacgagc ctgctgatcg ataccggcac gggcaccgac caggtgacga tcacggcgcc
    18301 cacggcggct cggatctacg gaggcccggg caacgacacg ctacgcggcg gcgcaggggc
    18361 aacacagttc ttcggccagg ccggaaacga cgcgctggtc gcgggcgccg gaggaggcaa
    18421 cgtactcgac ccgggcaccg ggacgaactc gctcgacgcg gcgaacggat ttgccgacac
    18481 cgtgaaccgc tgcaccggcc ttgacacggt cacgagcgac gtggcggaca cgctgctcgg
    18541 agcctgcccg acgaccgatg tgccctcgcc gctcccgccg cccgactttc cggcgcgacc
    18601 cacgcctgcc ccgcacgatc cggccggggg ccccacgcca tccggcgtgc tgctcagctc
    18661 gctgcggctc atcccggcgt cgttccgcgc cgccccgcgg ggagcgagct tcgccgtgcg
    18721 caccggcgcg cgtctcagct tccgcttgag cgcggcggcc gacgtcacgt tccgggtgga
    18781 caagctccgc agggggcggc accgcacctt ctggaccgct ctcggcagca acttcaagct
    18841 caggaagcgg cccgcgggaa taacccgcac tcgcttcacc ggccgcgtcc ccggcgcagt
    18901 gctcaggccc gggagctacc ggctggtcgc cgcgccggtg gatcccgacg gcagccaggg
    18961 cggcaccacg aaggtgcgct tccgcatcgt cgcctcgccg aagaagcgct agaccggctt
    19021 cgtcgtcatc gcccagaccg cgacgaggta ggcgatcacg agcacggcca cgaggaacgc
    19081 cgacgcgacg ccgaggccgc cgggcgtgcc gccttcacgg cgcgtgcgcc gaaccgcgag
    19141 ccaggcgagc agcgtggcga ccaccagaac cgcgagaccg gcgtcggcgg tggtgaagcc
    19201 gatcccgacc cacgatgcgt tcgagtcctc caggtgctcc ttggaggcga tccactgcgc
    19261 accgccgcgc atgatgatcc aggcaggaag gacgcccagc agcatcgtgc ggtagcccac
    19321 catcgcaagc gtggcattgc catcgcgcca cgcgaacagc agcgcggacc cggcgagcac
    19381 gagcgcgccg acgagcgtgg tggcgccgat cagatgcacg aggagcggga agttccacga
    19441 gtcgggacga atggcagcga gcacgtccat gtcagtgccc cggcttgtag accatgtcga
    19501 tgagcagaag gagcaccgcg agcgtccgca gccagtggag cgtcacggcc tgtgagggca
    19561 gcgcgacggc cgtcccgccc tcgcgcgcgg agaacgcctc attgaacgcc gcctgcacgc
    19621 ggccgcccag cccggaggcg atcacccaca gcacgatcgc cgcgatcacc cagccgtcga
    19681 gcaggtcgta gccgtccacg tagatcgcga gccagacgcc gaacacaatc gtgccgagtc
    19741 ctccgatcgc ccacagcacg ttgccgatcg agaggatccg cgcgtcgccc ctgaggcgga
    19801 gcacgaagcc gctgaagatc accaccgtcg cgatcagcgc gaacgcagac agcatgtgca
    19861 ggaagagcag cgtgtcgtac acgcgcggaa ccctaaaggg cggcttggac gctatgcggc
    19921 tacggacggc tcttcgccgg ccaccgtgcc gcgcgcgagc tcggcgtagc gcccgtcgag
    19981 ctcgagcagc tcggcgtgcg tgccgcgttc gacgatccga ccgtggtcga gcacgaggat
    20041 ctggtcggcg tcgcgcacgg tcgagaggcg gtgcgcgatc gcgatcgtcg tgcgcccgcg
    20101 cgcgagctcg tcgagcgcct cctgcagcgc ccgctcggtc tcgttgtcga gcgcgctcgt
    20161 ggcctcgtcg aggatgagca cgggcgggtt gcggaggatc gtccgggcga tggcgatgcg
    20221 ctgcttctca ccgccggaga agcggtagcc gcgctcgccc accatcgtct cgtagccgtc
    20281 ggggagcgac acgatgagat cgtggatctg ggcggcgcgt gccgcctgct cgatctccgc
    20341 gtcggtcgcg tcgggccgtg cgaagcgcag attctcgcgg atgctcgcgt ggaagaggta
    20401 cgtctcctgc gacaccagcc cgacggtggc ggcaagcgaa gagaggctcg cctcggccac
    20461 gtcaacgccg tcgatcgtga cccgcccgcg ctctggctca tacaggcgcg cgaccaggta
    20521 ggccagtgtc gtcttacccg agccggtctc accgacgatg gcggtggtgg tcccggcggg
    20581 cacgaccgcg tcgacgtcgg cgagcgtcca cggcgcgtcc tcgtcatagc ggaaccacac
    20641 gtcgtcgaag gtcacgtcgc cgcgcacgtc gcggagctcg accggatgcc ggcgctcgtc
    20701 gatgtccacc ggcaggtcga ggtactcgaa gatgcgcgcg aacatcgcca gcgacgtctg
    20761 cacgtcgagc cccaccgaca ggagcgactg gatcgggaac agcagccgcg tctgcagcgt
    20821 cgtgaacgcg accaccgtgc cgatcgagat cgcgtggccg ccgctcgcga tgttcattcc
    20881 ggcgaaccag tagaccgcgg ccggcaggat cgcgaagctc atctggacgg atgccatccg
    20941 ccagcggccc gccatgcggc tgcggacctc gatgtctgcg agctctgccg actcgtcacg
    21001 gaagcgcccc gcgagctccg ggccgcggcc catcgtcttg ccgagcagga tgccggacac
    21061 cgacagcgac tcctcgacca gcgtcgacat gtcggcgagg cgcccctggc gcaccgacgt
    21121 gatgcgcttg cgctccttgc cgactcgccg cgtgagccag acgaagaacg gcaacaggca
    21181 gagcgagaac gccgcgagtc gccagtcgag cgcgatcatc gcgacgagcg tggcgagcac
    21241 cgtcgtgacg ttcgacatga tcgacgtggc ggtgctcgtc acgacgttgt cgatcccgcc
    21301 gatgtcgttg gcgatgcgcg actgaacctc gcccgtgtgc gtgcgcgtga agaacgcgag
    21361 cgacatgcgc tgcaggtgcg agtagaccgc cgagcgcagg tcgtgcatca cgcgctgccc
    21421 gaccgtgttc gacaaccaag tctgcgcgac cccgatcacg ccgctcagca ccgagatggc
    21481 gatcatgccg agcaccagga acgtgagcag ctcggtgtcc ttgttcggga tcgcgtcgtc
    21541 gagcacggcg cgaaggagga acgggctgat gacgcctacg ccggccgcga ccacgatcaa
    21601 gagcagcagc acggagagcc gcccgcggta ggagcggaag agccgtgcta tgcgcttgag
    21661 tggaacgcgc gcctcaggcg gcagcgccgg gccagaagaa gccattaaga gagcgtagtg
    21721 aggcgcgtcg agcgctcagg ggagcaggcg cggcatgggg tcgaccggga cgccacggtg
    21781 gcgcgtctcg aaatgcaggt gcgggcccgt cgcacggccc gtcgagccga cgtacccgat
    21841 cagcgtgccg ccgctcaccg cctgaccctg cgcgaccgtg atcgaggaga ggtgggcgta
    21901 ccacgtctgc cagtggagtc gatgctgaac gactacgagg ttgccgtatc cgccgtcgtc
    21961 ccagccggca gatgcgacga cgcccacgcc ggcggcgccc acgggcgcgc ccgcgtcggc
    22021 gagaaagtcg atcccggtgt gccggcgacc ggacacccag ccgaacccgt cgccgatcgg
    22081 cgcgtcgacg ggtcgcagga agcgcaccgg tccgcgcggg gcgccgtggg cccgcgcccg
    22141 gcggcgcggg tgcttcagcg ccttgatcgt ggccgggccc gcgattccat cggacctcag
    22201 cccctgcgcg cgttggaagc ga
//
LOCUS       DASVXS010000066        17657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1360260, whole genome shotgun sequence.
ACCESSION   DASVXS010000066 DASVXS010000000
VERSION     DASVXS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17657
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360260"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VF752_03640"
     CDS             complement(<1..1794)
                     /locus_tag="VF752_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HEX8854669.1"
                     /translation="MTMSRATRPSPPTGQGRLAEVLASRGGKAAPEAPFVIEHREALI
                     YMLCEAAELEHGIMCQYLFAAFSLKQREDEGLTRGELAAVDRWRKAISHVATEEMLHL
                     ALVHNLLSAIGAAPHFGRPNLPAPAHHYPAGVNLTLVPFGEQALQHFIFLERPEGMEY
                     EGAEGLDMPAHEAVPLMAERDIVPQPQDFATVGHLYRSIEQGLAHLSEKFGEQNLFVG
                     PQRAQAIPENFGWDELVTVTDLASAQQAIDTILEQGEGARGRWQHAHFGQFVQILDEY
                     RAMVAANPNFDPVRPVMFATVRRCEHDDTVPRIGERVTSRCGDLFNVSYELLLQIFER
                     YFAHTEETDAQLGTLADATIALMLRVLKPLGNLITTLPVGPEYPGMNAGPSFELFYEN
                     DYLMPHRDAAWALLEERLREAANFCGLIQEIASEQVAGELASVHKALGDVADSLAGRF
                     AEWGATSRFARDAEQEPSSDGRAAKALARRAGALARTVASADGGGTSTKRLVSLFSAA
                     HAAFDQAGNREPAKRLLESVLRPLAEAVAGKRLRARTNIAAQGGADNAAALDGLLWAL
                     AQDATRLLGRWRGDPQRRTLLMEATAALQDLS"
     gene            complement(2032..2274)
                     /locus_tag="VF752_03645"
     CDS             complement(2032..2274)
                     /locus_tag="VF752_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854670.1"
                     /translation="MDGEQGETIYYLSAEGPREVVDERARWLREQRTPIESGPTEYDY
                     TPGYYAVFFYDPDGIKLERMHRPTYWEWLRGAPDDP"
     gene            complement(2424..3104)
                     /locus_tag="VF752_03650"
     CDS             complement(2424..3104)
                     /locus_tag="VF752_03650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8854671.1"
                     /translation="MHELASSQPLRHPSGDARRYLPGMGRIWLLPLYDPFTRLIGIEP
                     VHRKLADQAELASAQRVLEIGCGTGNLALLVKRMRPQLEVAGLDPDPKALARAARKAR
                     RAGIDLKLVRGFADQLPYPDASFDRVLSALMFHHLEADLRVASLREVLRVLRPGGSLH
                     LVDFGGNSHNLHGLARLSRRTHTLQDNWDDRIPTLMRDAGLSDPTEIDHLTRRIGRLT
                     YYRATRAE"
     gene            3302..4141
                     /locus_tag="VF752_03655"
     CDS             3302..4141
                     /locus_tag="VF752_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8854672.1"
                     /translation="MLRFSAADLPARDHAHAHDFLVLAYCERGGGTIGLDNRQWPLAA
                     GDAFLIAPGEVVRLSDHGHGMAAEGWSVFFPPEIIASGALGGSLGWAAHPLLFPFVGR
                     TAGGAQRLRVPPSERAAWSGHVAAIERELRRPRDGSSEAALAHLTLLLVSVARISVDL
                     GDHLRLRSEPLLAAVFEFIEAHYHEPISLSDIAAAVGLTAGHLTTVVRRKTGRPAQRW
                     ITERRMAEARRLLRETDLTVEAIATRTGYRQPSFFIKQFRRDHTLTPAAWRRSTRNPT
                     QPP"
     gene            complement(4317..4610)
                     /locus_tag="VF752_03660"
     CDS             complement(4317..4610)
                     /locus_tag="VF752_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854673.1"
                     /translation="MATTPESEFRELDRRTNDGIDVRLLWNSHTNRVSVTVKDERSGE
                     SFEFEIDSADALAGFHHPFAHAQPARPPGAMLAWLAGGHGEADDIDARAGKRQ"
     gene            complement(4730..6073)
                     /locus_tag="VF752_03665"
     CDS             complement(4730..6073)
                     /locus_tag="VF752_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8854674.1"
                     /translation="MTENLTELRALVTGEVLGPSDPEFEQARLCFNLLIDRRPAAIAR
                     CANARDVATALAFAQEHDLEIAVRGGGHNPAGHCAVDDGLVIDLSRMRKVEVDADARL
                     ARSEGGATWLDFDTATQARGVVAPGGVVGSTGVAGLTLGGGIGHLTAQCGLTCDNLVA
                     AELVTPTGEVIHANGDETPELLWALRGGGGNFGVATLLTFRLHPLSTVVGGRFDYAGE
                     GVREALRTFRNVDAGADDDLSCQAQLKLDEAVIPTLTVAPCYTGSDENPEEHRALRAA
                     PGLVSDGVRRHGFLDQQRVFDPAYGIDRNYWKSHFVRELPDELIDLLIDSMSALGRPP
                     GAILIESLRGAPKRIDPGSAALGYRGAAFNVSVMASWIDPALDDEEIAWARETAAAIE
                     PWSVSGGYANYMQADEPLERISAAYGPESFGRLRVLKSRYDPTNVLHRNQNIPPL"
     gene            complement(6134..6967)
                     /locus_tag="VF752_03670"
     CDS             complement(6134..6967)
                     /locus_tag="VF752_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8854675.1"
                     /translation="MTFAALAWGSEAAPLALMVHGYPDTAWTWRHLGPYLAERGWHAV
                     APFTRGYAPTDLAPNDSYMIADQFDDVLALHTAIGGDQRAVLIGHDWGAATLWALTER
                     EPTRFHRYVAIAVPPTPVLLRPWTSRKTLGDAARQARMSWYFLYNQLPGLSERGLDRV
                     VPKLWRDWSPGYDASEDLDHVWESLGGPGRRRAALRYYRNNLQKGLVKSFTIKAGAPA
                     LSLHGATDGCMNPKIAADNTDALPPGSRFELLPGVGHFVQLEDPPRVNALISDWIGSP
                     S"
     gene            complement(7027..7956)
                     /locus_tag="VF752_03675"
     CDS             complement(7027..7956)
                     /locus_tag="VF752_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8854676.1"
                     /translation="MPSKQSEAVKNLYRSWTEARITDPDRSLEEQRDDLERWGDLTAE
                     PGGVDYIETDAGEVPAMWAVPKGCVEDRVILCLHGGGFVGGSMYTHRKLFGHLAKTIG
                     ARALIPNYRRTPEHPHPAPVDDATAAYRWLLEQGVSANHIALTGDSSGGGLAITAMLH
                     ARVLGLPTAAAFMLISPWVDMELTGNSYESNREKDAFFYKEVVQGLVDMFLGERGNGK
                     DPLANPLYADLTGLPPIHIQVGGDETLLDDSRRLAEHARGAGVDVRVEIFPDQQHTFH
                     MAAGRAPEADEAIGALARWVRPKLGLADAARAA"
     gene            8088..9011
                     /locus_tag="VF752_03680"
     CDS             8088..9011
                     /locus_tag="VF752_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8854677.1"
                     /translation="MRPDVRQLQYFIAVAEELNFTRAAERLNLVQQSLSTAIAQLEAL
                     LGIKLFERTTRSVTLTNAGAAWLPYARDALAAAERAAAAARDLAAGRAGQLRVGLAAT
                     AALDVTPRLLRTFAERFPLVELAIEHFDCQDPSGGLRERRSDVAIVRPPFNDDGLELL
                     VIATEPRFAVLAADHPLADRAALDFAELADEPWMDIATDPIWWDFWRVAELRSGPPKV
                     GAICRSCEELLEAARAGTATGLVGESVVRAQSWSQLAFVEVRDIAPSTVAVAWHSDNH
                     QLAVRNFVDLAIDLSTPSPSSSGPSAQPAAM"
     gene            complement(9213..9848)
                     /locus_tag="VF752_03685"
     CDS             complement(9213..9848)
                     /locus_tag="VF752_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8854678.1"
                     /translation="MQLEQEVIERVRAQFGRPTGFPGRIAGWVMAHRSSNLRRNAWAV
                     SLLEVQRDDRVLEIGFGPGIAIRELARLAPDGYVCGLDHSEVMLRQAARRNADGVRLG
                     RVELRLGSVERLPAFEAPFDKILAVNALQFLDQPVEPLRELRRGLRSGGRIAIAFQPR
                     GPGASDHAARTRGQKIAAALRDAGFSQVRLETLELKPAVVCALGVNASGDD"
     gene            9921..10334
                     /locus_tag="VF752_03690"
     CDS             9921..10334
                     /locus_tag="VF752_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8854679.1"
                     /translation="MPDNDARLAIGELARRAGTAPSALRYYERIGLLPPADRAGGKRH
                     YSPASAERLALVRLYQYAGFTLKEIRKLLAAGSRRGASWTSLAEHKIAELDARIAEAQ
                     RAKDLLEHVLNCPHHDLLTCPNFRAAFQARLEAGS"
     gene            complement(10377..10829)
                     /locus_tag="VF752_03695"
     CDS             complement(10377..10829)
                     /locus_tag="VF752_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854680.1"
                     /translation="MPNESTEALGRLEALIGRWRTEGWTTDASGGLGGRIDAVDTYER
                     LPGGALLHIVDARVGEQRVEGAELIGYDPASASYVTHYFGSDGPNAYEARLVDEDGAL
                     VWTMRSERDRFTGSFNDARDVITSHWEALDADSGWQPWMDVALTKDAS"
     gene            complement(10819..11373)
                     /locus_tag="VF752_03700"
     CDS             complement(10819..11373)
                     /locus_tag="VF752_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8854681.1"
                     /translation="MTDGPLSKSSKTSAGLLLYRLRDDRLEVFIAHMGGPFWASKDEG
                     GWSMVKGEYQDDEDPFTAACREFLEETGSHPPPGRTLELGEIKQPSGKRIVAWAIESD
                     FNEASVRSNTFRLEWPRGSGQQREFPEIDRAGWFDTATARQKLVKGQMPFIEVLERQV
                     LGVEQNQEHAEAWQADREGDPNAE"
     gene            complement(11370..12395)
                     /locus_tag="VF752_03705"
     CDS             complement(11370..12395)
                     /locus_tag="VF752_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8854682.1"
                     /translation="MTEVGPAVHAWLQPNGLLGESNAGLVIGEGASLLVDTLWDARLT
                     RRMLAAMAPLIEKAPIETLVNTHSDGDHWWGNQEVQGAEIIATESATAVMEAQSPTEM
                     KRFRALTGALRLAGSLPLPYPRRADAAVIAAYAGEALKPFDFDGVRLVQPTRRFSGEL
                     VLDVGGREVRLLEVGPAHTLGDLIVWVPDAHIAFAADILFIGITPIMWAGPLERWVAA
                     LERLLELDAERFVPGHGPVCGPDEVRRLVDYWRWLDQAARQRLAAGTSPSDTAREIVL
                     GDEIAERGFADWLAPERALVSVRTIDAHRRGVANLPGPRELIAAFFGMALLARDHAAR
                     RGVRQMP"
     gene            12543..13475
                     /locus_tag="VF752_03710"
     CDS             12543..13475
                     /locus_tag="VF752_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8854683.1"
                     /translation="MSTQDQTRSDLARLLWLSVAAAVATISLKTVAWLVTGSVGLFSD
                     AAESVVNLVAAVFALVVVHWASRPPDEEHAYGHEKADYLSAGVEGALILFAAVGIAYS
                     AIGRLINPEPLSDVALGLGVSVAASAINLFVARKLITTGRSSNSIVLEADGRHLMTDV
                     WTSAGVVVGVAAVAVSGWDRLDAIIALVVAANIVATGISLIQRFTGGLMDRALEPQER
                     AAVESVLADFAGEDVEFHALRTRRAGQRAFVSVHVLVPGEWSVKRGHDLVEQIEGRLR
                     DVLRHATVFTHLEPVEDPKSFADTELDRREKPAA"
     gene            complement(13508..14884)
                     /locus_tag="VF752_03715"
     CDS             complement(13508..14884)
                     /locus_tag="VF752_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8854684.1"
                     /translation="MRDWTRRGCAIAGAVACVCVLASPGTAHAVTLAAAGNIACGPVQ
                     TPSASECQQGATASLVSTLDPDAVAALGDQQYDRGTSSEYSQFYANTWGTLTLPVHPV
                     PGNHEYMDPAGNAAGYFGYFGPAAGDPSTGYYSYDLGSWHIIALNSACNVINNCAPGS
                     AQEQWLRADLAAHVGVCTLAYWHHPLFTSGETSGDPNNAATRSFWQALYEHGADVVLN
                     AHDHSYQRFAPQTPNGASDPAHGIREFIVGTGGRSLFTGTVPAANLEFRDVSNFGVLL
                     LTLDADHYSWQFRTQSGGVTDPGSASCHKTAPPSPSPPPASPPPRSSPPSSGTPPSSR
                     PPSSSSPLSHSAVLRLSAHWVTINVRTGKGAVRARCGNAPADRCVVALTLFRRGTRIG
                     TMSGTIAGGKFGTLRVKLNRTGRLMLRRSKTYRLGVNAVGASRNVAGQATGIDRPLRL
                     KGTARRHR"
     gene            15209..16714
                     /locus_tag="VF752_03720"
     CDS             15209..16714
                     /locus_tag="VF752_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8854685.1"
                     /translation="MSAARWGAGQVQAQVVDAVGGPARARVILLFGGVLALNTADAST
                     VGAVAAQLEPDLHIGNTKVGLLTSVSLIVGALAAIPIGILVDRIHRVRLLSISIVLWS
                     VATFAGGLSGTYSHLLLSRLALGAVAATAGPAIASLTGDYFPVRERVKVYGYILSGEI
                     AGSAVGFLVSGAIAGLLSWRWAFFALSLPGFWLARALWRDLPEPRRGGTSRLERGAVD
                     LVAAAENPAGDQQPEEFVRDDEAAQEAVRARGIRPDPELVLTEDAAGMPLRRAVPYVL
                     RVRTNVELIISSALGYFFLAGLQTFAVVFIRAHYGASQTTATGVLGLLVLGALVGTLV
                     SGPLTDWLLRRGLISARIWVPAISYMVAAVLLVFGIINTSLTPAIWFFMAGAAALSAA
                     NPPLDAARLDIMPPGLWGRAESVRTVLRTVGQAIAPLLFGAVADLVAGIAPEQAPVGT
                     KPALPPGTGRGLEVSFLLMLIALAAAGYILLRARRTYPQDVATAAASASNA"
     gene            complement(16722..>17657)
                     /locus_tag="VF752_03725"
     CDS             complement(16722..>17657)
                     /locus_tag="VF752_03725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX8854686.1"
                     /translation="SLMSELYHPNDLLAQAARAEAAGFDFVTISDHIHPWLYSHHHSP
                     YAWSVLGALAAQTRELEMVSFVTCPTIRYHPVIVAQKAATVAAMSGGRFHLGLGAGEN
                     LNEHVVGRGWPPASLRHEMLEEAIEIIKALWSGGFHSHEGRHYTFHDARVYSLPDEPP
                     PIHVAASGRESVALAARAGGGLIATEPRAELTRSFDEATGGGRRKYAQPAVAVDDDEA
                     RARRLAHELFRFSAGAWKVQSELPNPINFEAATANVREEDIAEMVSCGPDPERHAEAL
                     RKWTDAGFDHIAIIQVGDPERFFQMWQSELRPRLD"
BASE COUNT         2589 a         6045 c         6022 g         3001 t
ORIGIN      
        1 ggacaggtcc tggagcgccg ccgttgcttc catcagcagc gtcctccgct gaggatcgcc
       61 gcgccaccgc ccgagcagcc tggtcgcgtc ctgggctagc gcccacagga ggccgtcgag
      121 cgcggcggcg ttgtcggcac cgccctgggc ggcgatgttg gttcgcgccc gaagccgctt
      181 gccggcaacg gcctcggcga gcgggcggag aacactctcg agcaggcgct tcgcgggctc
      241 gcgattgccg gcctgatcga acgcggcatg cgccgcgctg aagagactta caagccgctt
      301 ggtgctcgtg cccccgccgt cggccgacgc gacggtccga gcaagcgccc ccgctcgtct
      361 cgcgagcgcc ttcgccgccc ggccgtcgga actgggctcc tgctcggcgt cacgtgcgaa
      421 ccggctcgtg gctccccact cggcgaagcg gcccgccagc gagtcggcga cgtcgcccag
      481 tgctttgtgt accgacgcga gctcgcccgc gacctgctct gaggcgatct cctggatcaa
      541 tccgcagaag ttggcggcct cgcggaggcg ctcctcgagc agggcccacg ccgcatcacg
      601 gtggggcatc aggtagtcgt tctcgtagaa aagctcgaag ctcgggcccg cgttcattcc
      661 cgggtactcg ggcccgacgg gcagtgtcgt gatcaggttc ccgagcggct tcaaaacccg
      721 gagcatgagc gcgatcgtcg cgtcggccag cgtccccagc tgcgcatcgg tctcctcggt
      781 gtgcgcgaag tagcgctcga agatctgcag caggagctcg tagctgacgt tgaacaggtc
      841 gccgcatctc gaggtgaccc gctcgccgat ccgcggcacg gtgtcgtcgt gctcgcaccg
      901 gcgcaccgtc gcgaacatca ccggtcgaac gggatcgaag ttcgggttcg ccgcgaccat
      961 cgcgcggtac tcgtcgagga tctgcacgaa ctggccgaaa tgcgcgtgct gccagcgccc
     1021 gcgtgcgccc tcgccctgct caaggatcgt gtcgatcgcc tgttgcgcgc tcgcaaggtc
     1081 cgtcaccgtc acaagctcat cccaaccgaa gttctccggg atcgcctgcg cccgctgagg
     1141 cccgacgaac aggttctgct cgccgaactt ctctgacagg tgcgcgaggc cctgctcgat
     1201 cgagcgatac aggtggccta ccgtcgcgaa gtcctgtggc tgggggacga tgtcgcgttc
     1261 ggccatcagc ggcaccgcct cgtgcgccgg catgtcgagc ccttcggcgc cctcgtactc
     1321 catcccctcc ggtcgctcga ggaagatgaa gtgctgaagc gcctgctccc cgaaaggcac
     1381 gagcgtcaag ttcacaccgg ccggatagtg gtgggcgggg gcaggcaggt tgggtcgtcc
     1441 gaagtgcggt gccgcaccga tcgccgagag caggttgtgt accagcgcca ggtggagcat
     1501 ctcctcggtc gccacatgcg agatcgcctt tcgccagcga tcgaccgcgg caagctcgcc
     1561 gcgtgtcagc ccctcgtcct cacgctgctt gagcgagaag gcggcgaaca ggtactggca
     1621 catgatcccg tgctcgagct ccgccgcctc acagagcatg tagatcagcg cctcgcggtg
     1681 ctcgatcacg aacggcgcct cgggtgctgc cttgccgccg cgtgaggcca ggacctccgc
     1741 gagacggccc tgtccggttg ggggggacgg gcgggtggct cgcgacatcg tcatcggaaa
     1801 ggctggcgtc ccatgctgct caaatttctc agagtccgag ggcctcgatc aagggctgag
     1861 tgaccctccg gcgcgccgcg ctccttgatc gacgcttctg gctgccgtgg cccactggcc
     1921 aaataggctg aacctgcgtc cgggcgcgag caacacacgt cgggtctcga tctacaccgc
     1981 ttgggtatag agcgctcgaa ccgggcctaa gctcttgtgt cgcgcccgca gctaggggtc
     2041 gtcgggggcg ccgcgcagcc actcccagta cgtcgggcgg tgcatccgtt cgagcttgat
     2101 cccgtccggg tcgtagaaga agaccgcgta gtagcccggc gtgtagtcgt actcggtggg
     2161 cccgctctcg atcggggtgc gctgctcgcg gagccaccga gcgcgttcat cgacgacctc
     2221 gcgcgggcct tcggcggaga ggtagtagat cgtctcgccc tgctcgccat ccacctcgcg
     2281 aaggcccgac cagccgatcg gctcgagcag gccgcgatag aagggcaggc ttcgctcgtt
     2341 cgagctgacc gagatcgacg tggctgatct tcgagagcgg cgctttcacg tggtcgcctg
     2401 cgcgccggcg cggggtggcc ttctcactcg gcacgggtcg cgcggtagta ggtgaggcgg
     2461 ccgatgcgcc tggtgaggtg atcgatttcg gtggggtcgc tgagtcccgc gtcgcgcatc
     2521 agggtgggga tgcggtcgtc ccagttgtct tgcagggtgt gagtgcggcg tgagaggcgg
     2581 gccagcccgt gcagattgtg gctgttgccg ccaaagtcga cgaggtgcaa tgagccgccg
     2641 gggcgtagca cgcgcaggac ttctcgcagt gacgccacgc ggaggtctgc ctcgagatgg
     2701 tggaacatga gcgccgagag cacgcggtcg aagctcgcgt cgggataggg aagctggtcg
     2761 gcgaagccgc ggacgagctt gaggtcgatc ccggcgcggc gggccttgcg ggccgcgcgg
     2821 gcgagcgctt tggggtcggg gtcgagtccg gcgacctcga gctgcggccg catccgcttg
     2881 acgagtagcg cgagattgcc ggtgccgcag ccgatctcga gcacccgctg ggctgacgcg
     2941 agctcggctt gatctgcgag cttgcggtgg acgggctcga tcccgatcag gcgcgtgaac
     3001 gggtcgtaga gcggcaggag ccagatgcgg cccatgcccg gcagatagcg gcgggcgtcg
     3061 ccggagggat ggcggagcgg ttgcgatgaa gcgagctcgt gcattcgatt ctccgttggt
     3121 atgaggatgc ttagtgcttt gacgagtgat aatccctgtg ttgtcgggct tccagagcga
     3181 ttcgttggcc ggtatgcaac gatctgtcgt tctagtggag acgacacggc tgactgggcg
     3241 gcgggccgac ggggtcccga tctatgcata cgttcgcggc cctaacgcgc ctccagtcac
     3301 cgtgctgcgc ttctctgccg cggacctacc ggcgcgcgat cacgcgcacg cgcacgactt
     3361 cctcgtgctg gcgtactgcg agcgcggcgg gggcacgatc ggccttgaca accggcagtg
     3421 gccgctcgcg gctggtgacg cgtttctgat cgccccgggt gaggtcgtgc gcctgagcga
     3481 tcacggccac ggtatggcgg ccgagggctg gagcgtcttt ttcccgccgg agatcatcgc
     3541 ctccggagcg ctgggagggt cgctgggctg ggcagcgcat ccgctgctgt tcccgttcgt
     3601 cggccgcacg gcgggcggcg ctcagcggct acgcgtcccg ccgagcgagc gagcggcgtg
     3661 gtcaggtcac gtcgcggcga tcgagcggga actgcgcagg ccgcgcgatg gctccagcga
     3721 agcggcgctc gcgcacttga cgctgctgct ggtcagtgtc gcgcggatct cagttgatct
     3781 cggcgaccac ctgcggcttc gcagcgagcc gctgctagcg gccgtgttcg agttcatcga
     3841 ggctcactat cacgagccga tctcgctatc cgacatagcc gccgcggtcg ggctgacggc
     3901 aggtcacctg acgacggtcg tccgccgcaa gactgggcgc cccgcgcagc gctggatcac
     3961 cgaacgccgg atggccgaag ctcgcagact gctgcgcgaa accgatctaa ccgtcgaggc
     4021 gatcgcgacc cgcaccggct accgacaacc gagtttcttc atcaagcagt tccggcgcga
     4081 tcacacgctc acgcccgccg cgtggcgccg cagcacgcga aatccgacac agccgcctta
     4141 actcgccgcg cgcgatccac gcctgaggca cacctcgacc aaccccgcgc tggtgaggga
     4201 acggctcgga tgcgtccgcg ataacccggc gccggagcgg cacggcgact tcccgcgacg
     4261 cccagccacc caggcacgcc cggggacgag cacgagtggc ctgcgggcct cgtcgctcat
     4321 tgacgcttcc cggcgcgcgc gtcgatgtca tcggcttcgc cgtgcccgcc ggcgagccat
     4381 gcaagcatcg cgcccggcgg cctggccggt tgcgcgtggg cgaaggggtg gtggaatccg
     4441 gcgagcgcgt cggccgagtc gatctcgaac tcgaatgact cgccggagcg ctcgtccttg
     4501 acggtcaccg agacgcggtt tgtgtgtgag ttccagagca acctcacgtc gattccgtcg
     4561 ttcgtcctgc gatcaagttc gcggaactcg ctctccggtg ttgtggccat ggtcatgtcc
     4621 tgttcctctc gaggtgttcg ttggtcacag cgcatacggg ttcgttactc acttgacacg
     4681 gcgacccaag ccgatgtgac atgaccgctg tcgggccttc gagatcccgt cagagcggcg
     4741 ggatgttctg gttgcggtgc aggacgttgg tggggtcgta gcggctcttt agcacgcgga
     4801 gccggccgaa tgactcgggg ccgtaggcgg cgctgatccg ttcgagcggc tcgtccgcct
     4861 gcatgtagtt ggcgtagccg ccactgaccg accagggctc gatcgccgcc gccgtctcgc
     4921 gcgcccaggc gatctcctcg tcgtcgaggg ctgggtcgat ccagctggcc atgacgctga
     4981 cgttgaaggc cgccccgcgg tagccgagtg cggcactccc cgggtcgatc cgcttcggcg
     5041 cgccgcgcag cgactcgatc aggatcgcgc ccggcggccg gccgagcgcg ctcatgctgt
     5101 cgatcagcag gtcgatcagc tcatcgggaa gctcgcggac gaaatggctc ttccagtagt
     5161 tccggtcgat cccgtaggcg gggtcgaaga cccgctgctg atcgaggaac ccgtggcggc
     5221 ggacgccgtc gctgacgagc ccgggcgccg cacgcagcgc gcggtgctcc tccggattct
     5281 cgtccgagcc cgtgtagcag ggcgcgaccg tgagggtcgg gatcacggcc tcgtcgagct
     5341 tgagctgggc ctggcagctc agatcgtcgt cggcccccgc gtcgacattg cggaacgtcc
     5401 ggagcgcctc gcggacgcct tctccggcgt aatcgaagcg accgccgacc accgtcgaaa
     5461 gcggatggag gcggaaggtg agcagcgtcg ccacaccgaa gttgccaccg ccgccgcgca
     5521 gcgcccagag cagctcgggc gtctcgtcgc cgttggcgtg gatcacttcg cccgtgggcg
     5581 tgaccagctc ggcggcgacc aggttgtcgc aggtcaagcc gcactgcgcc gtcaggtggc
     5641 caatcccgcc gccgagcgtg aggcccgcga ccccggtcga gccgacgacg cccccgggcg
     5701 cgaccacgcc gcgggcctgc gtcgcagtgt cgaagtcgag ccaggtcgcc ccgccctcgg
     5761 agcgggctag ccgcgcgtcg gcatcgacct cgaccttccg catccgcgag aggtcgatca
     5821 cgagaccgtc gtccaccgcg cagtgcccag ccgggttgtg gccgccgccg cggaccgcga
     5881 tctcaaggtc atgctcctgg gcaaacgcca gagccgtcgc gacatctcgg gcattagcgc
     5941 accgtgcgat cgccgccggc cggcggtcga tgagcaggtt gaagcagagg cgcgcctgct
     6001 caaactcggg gtcggagggc cccagcacct cgccggtgac taaagcgcga agttcggtga
     6061 ggttctcggt catcaaacct ggagaccgca ttacgcacct gaactcatcg gcacgccatg
     6121 agatggctgg gcctcaggac ggcgagccga tccagtcgct gatcagcgcg ttgacccgtg
     6181 gaggatcctc gagctgcacg aagtgcccga cgccggggag caactcgaag cggcttccgg
     6241 gcggtagcgc gtcggtgttg tcggccgcga tcttggggtt catgcagccg tcggtggcac
     6301 cgtgcagtga cagcgccggc gcgcccgcct tgatcgtgaa cgacttgacg agccccttct
     6361 ggaggttgtt gcggtagtag cgcagcgccg cgcggcggcg tcccggccca cccagcgact
     6421 cccacacgtg atctaggtcc tcactcgcgt cgtaaccggg cgaccagtct cgccacagct
     6481 ttgggaccac gcggtcgagc ccgcgctccg acaatcccgg gagttggttg tagaggaagt
     6541 accagctcat ccgcgcctgc cgcgccgcgt cgccgagcgt cttgcgcgaa gtccagggtc
     6601 taagcagtac gggggtaggc ggcacggcaa tcgccacgta gcgatggaac cgggtcggct
     6661 cgcgctcggt cagcgcccac agcgtcgctg cgccccagtc gtgtccgatg agaacagccc
     6721 gctgatcgcc gccgatcgcg gtgtggagcg cgaggacgtc gtcgaactgg tcggcgatca
     6781 tgtagctgtc gttcggcgcc aggtcggtgg gtgcatagcc gcgggtgaac ggtgcgacgg
     6841 catgccagcc gcgctccgcc aggtacgggc cgaggtggcg ccaggtccag gccgtgtccg
     6901 gatagccgtg gaccatgagc gccagcggag ctgcctcgga accccacgcg agcgctgcga
     6961 acgtcatccc cgaggcttgc acacggatcg attggggctc ggcggcgctg gtgacgctca
     7021 ggctgttcac gcggcccggg cggcgtcggc gaggccgagc ttgggtcgta cccaccgggc
     7081 caacgcaccg atcgcttcgt cggcttcggg tgcgcgccct gcagccatat ggaaggtgtg
     7141 ctgctggtcg gggaagatct cgacgcggac atccacgccg gccccgcggg catgctcggc
     7201 gagccggcgg ctgtcgtcca agagcgtctc gtcaccgccc acctggatgt ggatcggcgg
     7261 taggccggtg aggtcggcat agagcgggtt ggcgagcggg tccttaccgt tgccgcgttc
     7321 gcccaggaac atgtctacca gcccctggac aacctccttg taaaagaagg cgtccttctc
     7381 ccggttcgac tcgtaggagt tcccagtgag ttccatgtcg acccagggcg agatcagcat
     7441 gaacgccgcc gcggtcggca ggcccagcac gcgggcgtgg agcattgcgg tgatggcgag
     7501 tccgccaccg gacgaatcgc ccgtgagcgc gatgtggttg gcgctgactc cctgttcgag
     7561 cagccagcgg taggcggctg tcgcgtcgtc gaccggcgcc gggtgcgggt gctcaggcgt
     7621 acggcggtag ttcgggatca gcgcccgggc gccgattgtc ttggcgaggt ggccgaagag
     7681 tttccggtgc gtatacatcg aaccgccgac aaagcccccg ccgtgtaggc agaggatcac
     7741 gcggtcctcg acgcatcctt tcggcaccgc ccacatcgcg ggcacttcgc cggcgtcggt
     7801 ttcgatgtag tcgacgccgc cgggctcggc ggtgaggtca ccccaacgct cgaggtcgtc
     7861 gcgctgctcc tcgagcgacc ggtccggatc cgttatccgg gcctcggtcc agctccggta
     7921 gaggttcttt accgcttctg attgcttgct tggcatggtg ctctcgcgcc aacgctgtcc
     7981 gatgatgtgt tggcgaacct aggcgtccgg ctcctaaacc ggaaacagaa cttggtggtg
     8041 tgctcacgcg gtgctgcgat gaatcggggg tggtaacttc gcctgcgatg cgtccggacg
     8101 tgcgtcagct tcagtacttc atcgcggtcg ctgaggagct gaacttcaca cgcgcggccg
     8161 agcgactcaa cttggtgcag cagtcgctga gcacggcgat cgcccagctc gaggcgctgc
     8221 tggggatcaa actattcgag cgcacgacgc gcagcgtgac gctgacgaac gccggagccg
     8281 cgtggcttcc gtacgcgcgc gatgccctcg ctgcggccga gcgggcggcc gcggcggcgc
     8341 gggatctggc ggccggtcga gcgggccagc ttcgcgtcgg gctcgcggcg acggcagcgc
     8401 tggacgtcac gccgcggctg ctgcgcacgt tcgccgaacg ctttccgctt gtagagctgg
     8461 cgattgagca cttcgactgc caagatccgt cgggcgggct ccgcgagcgc cgcagcgacg
     8521 tcgcgatcgt ccggccgccg ttcaacgacg atgggctcga gctgctcgtg attgcgacgg
     8581 agccccgctt tgccgtcttg gcggccgatc acccgctcgc cgaccgcgcg gccctcgact
     8641 tcgccgagct cgccgatgag ccgtggatgg atatcgcgac cgatccgatc tggtgggact
     8701 tctggcgggt cgccgagctg aggtcaggac cacccaaggt cggcgccatt tgtcgcagct
     8761 gcgaagaact cctcgaggcc gctcgggccg ggacggcgac cggccttgtt ggggaatcgg
     8821 tggtccgcgc gcagtcttgg tcgcagctcg cgttcgtcga ggtccgcgac atcgccccct
     8881 cgacggtcgc ggtcgcctgg cactcggata accaccagct tgccgtacgt aacttcgttg
     8941 acctcgcgat cgatctctcg acgccctcgc catcgtcttc gggtccgtcg gcccagccgg
     9001 ccgcgatgta gcgactgacc cgcctgcggc ctgctgagaa gcatctatcc ccaagagaac
     9061 ggtgcgcctc cgccgagctc agacgccttg gtgtggctgg ctaagtcgct gcatgaccag
     9121 ggcgtgcgct gcgcgcagct cgccctcggc ggggcggcac ctcagagccc atccaggatc
     9181 cgagtggacc gggcctcgcg cccgtgctgg agtcaatcgt cgcccgacgc attgacgccg
     9241 agcgcgcaca ctacggcggg cttgagctcg agcgtctcaa gccgaacctg cgagaagcct
     9301 gcgtcgcgca gcgccgcggc gatcttctga cctcgcgtcc tcgccgcgtg gtcgctcgcc
     9361 ccggggccgc gtggctgaaa ggcgatcgcg atccgacccc cgctcctcag cccgcggcgt
     9421 agttctctga gcggttcgac cggttggtcc aagaactgca gcgcgttgac agccaagatc
     9481 ttgtcgaaag gcgcctcaaa cgccggcaag cgctcgacag aaccgagcct cagctcgacc
     9541 cggccgagtc gcactccgtc cgcgttgcgc cgtgcagcct ggcgcagcat cacctcggag
     9601 tggtcgagcc cgcacacata gccgtctggg gcgaggcgcg ccagctcgcg gatcgcgatc
     9661 cccggcccga agccaatctc gagaacgcgg tcgtcgcgct gcacttcgag cagtgacacc
     9721 gcccaggcgt tgcgccgaag gttcgaggag cgatgggcca tgacccaacc cgcgatccgg
     9781 cccggaaagc cggtcggtcg gccgaactga gcgcggacgc gctctatgac ctcttgctcc
     9841 aactgcaccc tcggtacctc ctgtccccgt cgggtcccta tggtgcaggt tgaagcgcac
     9901 ttcaagtcaa ggggaaaccg atgcccgaca acgacgcccg gctcgccatc ggcgagctcg
     9961 cccggcgcgc cggaaccgcg ccctcggcgt tgcgctacta cgagcgaatc ggcctccttc
    10021 cgcccgccga ccgcgcgggc ggcaaacgcc actactcgcc cgcgagtgcc gagcgcctcg
    10081 ccctagtgcg cctctaccag tacgccggct tcacgctcaa agagatccgc aagctgctgg
    10141 ccgcaggcag tcgccgcggc gcgagctgga cctcgcttgc cgaacacaag atcgcggagc
    10201 tcgacgcccg tatcgccgag gcacaacgcg caaaggacct cctcgaacac gtcctcaact
    10261 gcccccatca cgaccttctc acctgcccca atttccgcgc cgcgttccag gcccgactcg
    10321 aagcgggctc ataacccgct cggtgcggaa ccagccacca acgccggcgc ccacaatcag
    10381 ctggcgtcct tggttagggc cacgtccatc cagggctgcc agccggagtc ggcgtcgagc
    10441 gcctcccagt ggctcgtgat gacgtcgcga gcgtcgttga aacttccggt aaaccggtcc
    10501 ctctcgctgc gcatggtcca caccagcgcc ccgtcctcgt cgaccaggcg cgcctcgtac
    10561 gcgttcggcc cgtcgctacc gaagtagtgc gtgacatagc tcgcgctggc cgggtcatag
    10621 ccgatgagct cggcgccctc gaccctctgc tctccgaccc gggcgtcgac gatgtgcagc
    10681 agcgccccgc ccggcaaccg ctcataggta tcgacagcat cgattcgccc ccccagacct
    10741 cccgaagcat cggtggtcca tccctcggtc ctccaccggc cgatcaaggc ctcgagcctc
    10801 cccagtgcct cagtggattc attcggcatt tgggtctcct tcgcgatctg cctgccaggc
    10861 ctcggcgtgc tcttggttct gttccacgcc gagaacctgg cgctcgagaa cctcgatgaa
    10921 cggcatctga cccttgacca gcttctgccg cgcggtcgcg gtgtcgaacc acccggcgcg
    10981 gtcgatctcc gggaattcgc gctgctgacc cgacccgcgc ggccactcga gtctgaacgt
    11041 attgctccga acgctcgcct cgttgaagtc gctctcgatc gcccacgcca cgattcgttt
    11101 gccgctcggt tgctttatct cgccgagctc gagcgttcgg ccgggcggtg gatggctgcc
    11161 ggtctcctcg aggaactcac gacaggcggc ggtgaacgga tcctcgtcgt cttggtattc
    11221 gcccttgacc atcgaccagc cgccctcgtc cttgctggcc caaaacggcc cgcccatgtg
    11281 ggcgatgaag acctcgagcc gatcgtcgcg cagccggtag agcagtaggc ccgcgctcgt
    11341 cttgctcgac ttgctcagtg gaccgtcggt cacggcatct gtcgcacgcc gcgccgcgcc
    11401 gcgtggtctc gcgccagtag cgccatgccg aagaacgcgg cgatcagctc gcgggggcca
    11461 ggtaggttgg cgacgccgcg acgatgtgcg tcgatcgtgc ggacgctgac cagcgcccgc
    11521 tccggagcaa gccagtcggc gaagccccgc tcggcgatct cgtcgccaag gacgatttca
    11581 cgcgcggtgt cagagggcga cgttcccgca gcgagacgtt gtctggccgc ctggtcgagc
    11641 cagcgccagt agtcaaccag gcggcgcacc tcgtcggggc cgcagactgg accgtgcccg
    11701 gggacgaacc gctcggcgtc gagctcgagc aaccgctcga gcgccgccac ccagcgttca
    11761 agcggcccgg cccacatgat cggggtgatg ccgatgaaga ggatgtcggc cgcgaacgcg
    11821 atgtgcgcgt ccggcaccca gacgatcagg tcacccaggg tgtgtgcggg gccgacctcg
    11881 agtaggcgaa cctctcgccc cccgacgtca agcaccaact ctccggagaa cctgcgggtc
    11941 ggctgtacaa gccgcacccc atcgaagtcg aagggcttga gcgcctcgcc cgcgtaggcc
    12001 gcgatcacag cggcgtcggc tcgccgcggg tagggaagcg gcagcgatcc ggcgagccgc
    12061 agggcgcccg tgagcgccct gaaccgcttc atctcagtcg gcgactgcgc ttccatcaca
    12121 gccgtggccg actcggtggc gatgatctcc gccccttgga cctcctggtt tccccaccag
    12181 tgatcgccgt cggagtgcgt gttaacaagc gtctcgatcg gcgccttctc aatcagcggc
    12241 gccatcgcgg cgagcatgcg gcgcgtaagc cgcgcatccc acagcgtgtc gacgagcagc
    12301 gacgcgccct cgccgatcac caggccggcg ttactctcgc cgagcagccc gttgggctgt
    12361 agccaagcat ggacggcagg gccgacctcg gtcaagccgc cctcgaatcg cgccgggcct
    12421 ggatgaagcg gtgtgaccgc aaccatcagt gcacccattc tgccgagacg agcgtgtcca
    12481 ggttcacgtc gtgcgtgccc cgccaaccct gcccctcatc gctgccaaga tccatctgag
    12541 tggtgagtac gcaggaccag acccgtagcg acctcgcgcg gcttctctgg ctgtcggtcg
    12601 ccgctgcggt cgcgacgatc agcctcaaga ccgtcgcctg gctcgtgacg ggctcggtag
    12661 gcctgttctc ggacgcggcc gagtcggtgg tgaacctggt ggccgcggtc ttcgccctcg
    12721 tcgtcgtgca ctgggcttct cggcctccgg acgaggagca cgcatacggt cacgagaagg
    12781 ccgactacct ctccgcgggc gtggagggcg cgctgatcct gttcgccgcg gtcgggatcg
    12841 cctactcagc catcggccgg ctgatcaacc ccgagccgct ttccgatgtt gcgctaggcc
    12901 tcggcgtgtc ggttgccgca tcggcaatca acctgttcgt tgcgcggaag ctgatcacga
    12961 ctggacgttc aagcaactcg atcgtgctcg aggccgacgg caggcacctc atgaccgacg
    13021 tttggacgtc cgccggcgtc gtcgtcggcg ttgcagcagt cgccgtctcg ggctgggacc
    13081 gcctcgacgc gatcatcgcg ctagtcgttg cggcgaatat cgtggcgacc gggatctcgc
    13141 tgattcagcg gttcaccggc ggactcatgg atcgcgccct cgagccgcag gagcgcgcgg
    13201 cggtcgagtc cgtcctcgcc gacttcgccg gcgaagacgt cgagtttcat gcgctacgaa
    13261 ctcggcgtgc tgggcagcgc gcgttcgtgt cggttcacgt gctcgtcccg ggtgagtggt
    13321 cggtgaagcg gggacacgat ctcgttgagc aaatcgaggg ccggctgcgc gatgtgcttc
    13381 gccacgcgac cgtcttcact catctcgaac cggtcgaaga tccgaagtcg ttcgcggaca
    13441 cggaactcga cagacgggag aagccggctg cgtgaccgac gacctggtgt cttcgtcgct
    13501 tacgcggtca gcggtgtcgc cgcgccgtgc ccttcagtct cagcggccgg tcgatccccg
    13561 ttgcttggcc cgccacgttc ctcgaggccc cgaccgcatt tacgccgagt ctgtaggtct
    13621 tgctgcggcg gagcatcagc cggccggtgc ggttcagctt cacgcgcaga gtcccgaact
    13681 tgccgcccgc gatcgtgccg ctcatcgtgc cgatcctcgt gcctcgtctg aacagcgtga
    13741 gcgcgacgac gcacctgtcc gcaggggcgt taccacacct ggctcggacc gcccccttgc
    13801 cggttctcac gttgatcgtc acccagtgcg cgctgaggcg aagtacggcg ctgtgggaaa
    13861 gcggcgacga cgaggagggt ggccgggagg acggcggtgt acctgaggac ggcggagacg
    13921 agcgcggcgg aggagacgcg ggtgggggag acggcgacgg aggggccgtc ttgtggcagc
    13981 tggcggaccc cggatcggta acgccgccgc tctgggtccg gaactgccac gagtaatggt
    14041 ccgcgtcgag cgtcagcagg aggacaccga agttgctgac gtctctgaat tcaagattcg
    14101 cggccggcac cgttcccgta aagaggcttc tgccgccagt cccgacgatg aactcgcgga
    14161 tcccgtgagc cgggtctgaa gcgccgtttg gcgtctgtgg cgcgaagcgc tgatagctgt
    14221 ggtcatgggc gttgagcacg acgtctgcgc cgtgctcgta gagggcctgc cagaacgagc
    14281 gagtcgctgc gttgttcgga tcgccgctcg tctcccctga ggtgaacagc gggtgatgcc
    14341 agtacgcgag ggtgcatacg ccgacgtggg ccgcgaggtc cgcgcgcagc cactgctcct
    14401 gggcggaacc cggcgcgcag ttgttgatca cgttgcaggc cgagttgaga gcaatgatgt
    14461 gccaggagcc aaggtcgtat gagtagtaac ccgtgctcgg gtcgcccgcc gcggggccga
    14521 agtagccgaa gtaacccgcg gcgttgccgg ccggatccat gtactcgtgg ttgccgggga
    14581 ccgggtggac cggcagcgtc agcgtccccc aggtgtttgc gtagaactgc gagtactccg
    14641 atgaggtccc gcggtcgtac tgctggtccc ccaacgctgc tacggcgtcg ggatccagcg
    14701 tggagaccag cgaggccgtc gcgccctgct ggcactcact cgcactcgga gtctgcactg
    14761 ggccgcacgc gatattgccg gccgccgcga gcgtgaccgc atgtgccgtt cctggcgatg
    14821 cgagcacaca cacacaggcc accgcgcccg cgatcgcgca cccgcgacga gtccaatctc
    14881 tcacccgggc tccttctacg tcgttctgct gggggtgttt cgaccgtacg cccgccgatc
    14941 aaagcgcgtc aaaccgctgt gcccttatgg gtagggacgc tccccttttg cgctccgggc
    15001 ctgctacgtg tgcgggaacg agtggcgctc atgggcgacg atccagcgcc cgttttcttt
    15061 actcagcccg agcctgagcc acaggcggtt cgccgatctg cttctcgtcg tcggacatgt
    15121 gccgttggag cgaccagcgc tctagcgtgg ggctatcgag ctaggcagcc tcctcagcga
    15181 catcccacgg cgcgcggtcg caggcgtagt gtcggctgcc cgctggggcg caggccaggt
    15241 tcaggcgcag gtcgtcgacg cggtcggggg tcctgcgcgc gctcgcgtga tcctcctgtt
    15301 cggcggcgtt ctggccctga acacggcgga cgcctcgacg gtcggcgcgg tggccgcgca
    15361 gctcgagccg gaccttcaca tcggcaacac gaaggtcggc ctgctcacgt ccgtctcgct
    15421 gatcgtgggc gccctggcgg cgatcccgat cggcatcctg gtcgaccgca tccaccgggt
    15481 ccgcctgctc tcgatcagca tcgttctctg gagcgttgcc acgttcgccg gcgggctttc
    15541 cgggacgtac tcgcacctgc tgctttcacg gctggcgctc ggtgcggtcg ccgccactgc
    15601 cggtcccgca atcgcctcgc tcaccggcga ctacttcccg gtccgcgagc gcgtcaaggt
    15661 ctacggctac atcctcagcg gggagatcgc cggcagcgcg gtcggcttcc tggtcagcgg
    15721 cgcaatcgcc gggctcttgt catggcggtg ggcgttcttc gccctctcgc taccggggtt
    15781 ctggcttgcg cgcgcgctgt ggcgcgatct gcctgaacct cgtcgcggag gaaccagccg
    15841 cctcgagcgc ggcgcggtcg acctggtggc tgccgcggag aatcccgccg gcgatcagca
    15901 gcctgaggag ttcgtccggg acgacgaggc cgcccaggag gcggtgcgag cgcgcggcat
    15961 tcgccccgac cctgagcttg tgttgaccga ggacgcggcc ggaatgccgc tccgccgtgc
    16021 cgtcccgtac gtgctccggg tccgcaccaa cgtggagctg atcatctcgt cggccctcgg
    16081 ctacttcttc ctcgccgggc tgcagacgtt cgccgtcgtc ttcatccgcg cccactacgg
    16141 ggcgagtcag accaccgcga ccggggtcct cggcctgctc gtgctcggcg cgctcgtagg
    16201 cacgctcgtg agtggtccgc tcaccgattg gctgttgcgc cgggggctca tcagcgcccg
    16261 catctgggtg cctgcgatct cttacatggt cgccgccgtg ctgctcgtct tcggcatcat
    16321 caacacctct ctgacgccgg ccatctggtt cttcatggcc ggcgcagcgg cgctctcggc
    16381 ggccaaccca ccgctcgacg ctgcgcgcct cgacatcatg cctccggggc tgtggggacg
    16441 ggccgagagc gtgcggaccg ttctccgtac ggtgggccag gcgatcgccc ctttgctgtt
    16501 cggcgcggtc gctgatctgg tggcaggcat agcgccggag caggcgccgg tcggcacgaa
    16561 gcccgcgctt ccgcctggca ccggccgcgg actcgaggtg agcttcctcc tgatgctcat
    16621 cgcgcttgcc gcggccggct acatcctgct ccgggctcgc cgcacctatc cgcaggacgt
    16681 tgcgaccgca gcagcgtcgg cgtcgaacgc gtgagagcgc gttagtcgag ccgcggccgc
    16741 agctcgctct gccacatctg gaagaagcgc tcggggtcgc cgacctgaat gatcgcgatg
    16801 tggtcgaagc cggcgtccgt ccacttccgc agcgcttcag catggcgctc gggatccgga
    16861 ccgcaggaga ccatctcagc gatgtcctcc tcgcgcacgt tcgcggtggc agcttcgaag
    16921 ttgatcgggt tcgggagctc ggactgcacc ttccatgcgc ccgcggagaa gcggaacagc
    16981 tcgtgcgcga gccgccgcgc gcgtgcctcg tcgtcgtcga ccgcgaccgc gggctgcgcg
    17041 tactttcgcc tgccgccgcc ggttgcctcg tcgaacgagc gagtgagctc ggcgcgcggc
    17101 tcggtggcga tcagaccgcc gccggcgcgt gccgcgaggg cgaccgactc gcgcccgctc
    17161 gccgcgacgt ggatcggcgg tggctcgtcc ggcaggctgt acacgcgggc gtcgtggaag
    17221 gtgtagtgcc tgccctcgtg cgagtggaag ccgcccgacc agagcgcctt gatgatctcg
    17281 atcgcttcct cgagcatctc gtggcgaagg ctcgccggcg gccagccgcg gcccaccacg
    17341 tgctcgttca ggttctcgcc ggcgccgagt ccgaggtgga agcgaccgcc gctcatcgcc
    17401 gccaccgtcg ccgccttctg ggccacgatc accgggtggt agcgaatggt ggggcaggtg
    17461 acgaacgaga ccatctcgag ctctcgcgtc tgcgcggcga gcgccccgag cacgctccac
    17521 gcgtacgggc tgtggtgatg cgagtacagc cacggatgga tgtggtcgga gatggtgacg
    17581 aagtcgaagc ccgccgcctc ggccctcgct gcttgggcta ggagatcgtt cgggtggtac
    17641 agctcggaca tgaggct
//
LOCUS       DASVXS010000067         9537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_857259, whole genome shotgun sequence.
ACCESSION   DASVXS010000067 DASVXS010000000
VERSION     DASVXS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857259"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF752_03730"
     CDS             <1..460
                     /locus_tag="VF752_03730"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8854687.1"
                     /translation="RGMAAQPAGLTFEIKRVLPVEPAVVFRSFSTAHELAQWWGPAGF
                     SVRSLDFDPGVGAGYRIEMQPPEGDPFRLTGEFTEIDPPVRLAFTFVWENPDPDDVET
                     LADLSFRDLDGSTEVVLTQGPFKTEGRRELHRDGWTDTFDKLEQHLANGG"
     gene            499..1050
                     /locus_tag="VF752_03735"
     CDS             499..1050
                     /locus_tag="VF752_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEX8854688.1"
                     /translation="MPDRLYYTNSDEANQMIASDPLALLVGFVLDQQITVQHAFTGPL
                     VMKERLGAFDADTLANADLEPVFREKPAIHRYPGSMARRVHDLAVHIRDNYDGDAARV
                     WTEATDSSQLRENLSALPGFGEMKIKSVGAVLSKRFGLAVADDLVPPHPTLGDVDSPQ
                     ALLDYQAAKRIHKAEWTKARAAS"
     gene            1481..2056
                     /locus_tag="VF752_03740"
     CDS             1481..2056
                     /locus_tag="VF752_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8854689.1"
                     /translation="MTRDGIPVTTIARTLLDLAAVLNERQLTYAIEQAERQQRFDKKA
                     ITEACKRNPRHSGLRALRAAVQAFEPELTHTRSDLEREFVSFCRDWDIPVPAFNVVVC
                     GYTVDAAWQRERLIVELDTRDYHDDGAGFEEDRRRDAELQRRGYRVIRITAKRLREEP
                     QAIAELLRELLAQANAGAPATTRLRPSSFAR"
     gene            complement(1996..3057)
                     /locus_tag="VF752_03745"
     CDS             complement(1996..3057)
                     /locus_tag="VF752_03745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8854690.1"
                     /translation="MVATGSSKPPGRQGSWLCADDFARRRLLDMERHLKPARSAVFAL
                     LAVSLMVLSPWVGWWTLGPLAGAVIVFVIADQMMVHSDRPEYWVAGAWAFCQLMIGVA
                     VVIQGGAGSSFFPWLAIPVATLSARFTARGMIVGVAWTAAIAVAVALSRQPSAVLHNP
                     QILVVPLSVIGGIAVLTTALMRSDVLHRAEAALDPLTGLFNRHALHRRAVELVEQAHL
                     TDAPIAVLVGDLDHFKEVNDEHGHLVGDNVLRDVAYILRTALRSFDLVYRYGGEEFVV
                     LLPGASQGDAVAIAERMRKAVSEARPGGLDISISFGVCSARGLALDFERLFHSADELL
                     YRAKLEGRNRVVAGAPAFA"
     gene            3188..4573
                     /locus_tag="VF752_03750"
     CDS             3188..4573
                     /locus_tag="VF752_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8854691.1"
                     /translation="MTTPTTRELDRGRIRALTARELDALNERTQKSGETYRRAEQVLS
                     GGVASSYQLREPWPIYLEYGEGARVWDVDGNEYFDFHNGFGSMVQGHAHPAIGAAVQK
                     RYAQGTHFAAPTEDAIVVGEELAKRFRLPRWRYTNSGSESTMDAIRIARGLTGRDDVL
                     KIFGSYHGHHDTVMVSIGVAYDQIGPPDRPNSLPYGAGIPQSTVDHVHAVHFNDAETM
                     DRRIAELDAEGRKPACVLMEAAMMNLGVVLPEDGYLEAVREITRKHGVVLIFDEVKTG
                     LTIAAGGAVERFGVEPDMVTLAKALGGGLPTGAIGMSEEVARVVEDGSVYQVGTYNGN
                     PLGMAAARANLQDVMTPDAYAHLHHLNDRILASCEEVVDRHGLPAYTVGIGSKGCVTF
                     AAEKVVDYPSFKRSQDPELSELAWLWSMNRGIFMTPGREEEWTLSVTHTDEAIDRYVS
                     VFAELAAELTA"
     gene            complement(4570..5424)
                     /locus_tag="VF752_03755"
     CDS             complement(4570..5424)
                     /locus_tag="VF752_03755"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8854692.1"
                     /translation="MDFEEIRYEVADNILTITLNRPDRLNAFTRKMGEELVAAFDAAD
                     ADDDVRAIVVTGEGRGFCAGADLEAGGATFDWRGRSEGESVPRDGGGQVVLRIHECTK
                     PVIAAINGPAVGVGITMTLPMDIRLVAEGTKIGFVFTRRGIVPEACSSWFLPRIVGIS
                     QAMEWAATGRVFSSEEALAGRLVRSLHPADQVLAEARALAAEIAENAAPVSVALARRL
                     MWAGLGFSHPMDAHRADSRAMFSRGQSADAVEGVTSFLEKRPPRFTDRVSDGLPEIFP
                     GREEPVFS"
     gene            5569..6387
                     /locus_tag="VF752_03760"
     CDS             5569..6387
                     /locus_tag="VF752_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEX8854693.1"
                     /translation="MNLRTAVGAAAVVTALCAGVTAQASASPLAHGHFVKIQKHRTKA
                     NANQSTNWFGYNQGTLEQGGKQFNSITGDWTVPTASQHTKDQDAYSSDWIGIGGGCVD
                     ANCTIGDSTLIQTGTEQDVDTAGNSSYSAWWELIPGPSIDISNFPVSPGDHMNAEIAE
                     TVAGANVWKITLKNVTKGQTFTTTVPYSSTHATAEWIEETPLLIGTSPGLAPLPNLTK
                     TPFSAGTTNGKNAGLKASEEIQLIDSNGHVIGTPSAPSATLDGLNVCAWATSCS"
     gene            complement(6433..7902)
                     /locus_tag="VF752_03765"
     CDS             complement(6433..7902)
                     /locus_tag="VF752_03765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8854694.1"
                     /translation="MALAPPLGMKVGRYRIEAEAGRGGMGVVYRARDPQLDRVVALKV
                     IAPELASDSTFRARFARESQVTAGLDHPNVIPVYDAGEDDGRLFIAMRFVEGTDLQRV
                     LRERGALEPALAVELISQAASALDAAHARGLVHRDVKPANLLIAGEERPHVYLTDFGL
                     AKRDGSSGQLTSTGGWLGTPDYSSPEQGEGRELGPASDVYSLGCVLYAALTGAAPFAD
                     VPRAGKGWAHMNERPPRLRDRRPELPAALEPVLARALAKDSRDRHHSAGELARAAQAA
                     IAGGRTRTLLQRRRPSGPSTARTTKLVTSRRRRRVAPALAALVLFAAAGLAAILLLPA
                     GGGSSDAGERAAKPAAPRPAPTPAANPVDTVSCAATECTQNGEKVITPIEGQTCGSGE
                     RAGSWSRIEADGAPMFACVADSPPAVPGVVTMPHIEGAQLDKAEDYLDSLGLDYSVSG
                     GGMLGVIIDSNYTVCATAPGAGSAIQPGSSARLFAEHSC"
     gene            7896..8438
                     /locus_tag="VF752_03770"
     CDS             7896..8438
                     /locus_tag="VF752_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX8854695.1"
                     /translation="MPLPGYSEHRRRQRVNSLSEGAVKDLLLHRPAWYVLGPLLGLVV
                     LGLYAAISERFGVVGGYSNVVERISGRLPKLGWKAWVLFGVLGGSLVYRLAAGRSNIG
                     DGYGWLTRTFGGSGGHLIVAGLLVAAGVLIGFGAKTAGGCTSGNGLGGCSAGSTSSFA
                     ATATFMGTAIVCSFLIKAIT"
     gene            8435..8905
                     /locus_tag="VF752_03775"
     CDS             8435..8905
                     /locus_tag="VF752_03775"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX8854696.1"
                     /translation="MTRTKATAALFGAAFGFLISWGQFADPDRIRDMLLLRDPYLYLM
                     MASAVAVGFVGLRLLTRRGARAFLTGRPITYERTRPQPRHFAGAAIFGVGWSIADSCP
                     APIAAQLTQGVVWSVFTIAGIWLGILLFLKRQERREQVPERRALGGLAVDKPPG"
     gene            8952..>9537
                     /locus_tag="VF752_03780"
     CDS             8952..>9537
                     /locus_tag="VF752_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8854697.1"
                     /translation="MSTDTASTRAAERDLLRTIAAGTATVTGRAFFRSLVHHVALALG
                     ADVAWASEMQAGPGERAVVIASWGREDIRLPEGHDYDLSQTPCEGTIERDVVSIPENV
                     GEAYPNDTFIAEHGLSGYLAVVMRSSDDERLGYIGVMSRGRLEATEDELAVLSIFASR
                     AAAEIERRRHEVALRAREAEVAQSRSRVVSAADEE"
BASE COUNT         1538 a         3292 c         3295 g         1412 t
ORIGIN      
        1 tcgagggatg gcggcgcagc cagccgggct gaccttcgag atcaagcgcg tccttcccgt
       61 cgagcctgcc gttgtatttc gctcattcag cactgcgcac gagctcgcgc agtggtgggg
      121 gccggctggg ttcagcgttc ggagcctcga cttcgaccct ggcgtcggag ccgggtatcg
      181 cattgagatg cagccgcccg agggcgatcc gttccgtctg acgggcgagt tcaccgagat
      241 cgatccgccc gtgcgcctcg ccttcacgtt cgtatgggag aacccggatc cagacgatgt
      301 cgagaccttg gcggatctct cgtttcgtga tctggatggc tcgacggagg tcgtcctcac
      361 tcagggtccc ttcaagaccg aagggcgccg cgagcttcac cgggacggct ggacggacac
      421 gttcgacaag ctcgagcagc acctggcgaa cggcgggtag tgccggcgcc cggtgccttc
      481 tcgtctacga tcggctaggt gccggaccgc ctctactaca cgaactccga cgaagcgaac
      541 cagatgatcg cctcggaccc gctggcgttg ctggtggggt ttgtcctcga ccagcagatc
      601 actgtgcagc acgcgttcac cggtcccctc gtgatgaagg agcggctggg cgcgttcgac
      661 gccgacacac tcgccaacgc ggatctcgag cccgtgttcc gcgagaagcc ggcgatccac
      721 cgctaccccg gctcgatggc gcggcgagtg catgacctcg ctgtccacat acgcgacaac
      781 tacgacggcg acgctgcgcg tgtgtggacc gaggcgaccg actcatcgca gctccgggag
      841 aacctgtcgg cgctgcccgg cttcggcgag atgaagatca agtcggtcgg cgccgtgctc
      901 tctaaacgat ttgggctcgc ggttgcggac gacctcgtgc cgccgcatcc caccctgggc
      961 gacgtcgact ccccgcaggc gcttctcgac taccaggcgg cgaagcgcat ccacaaggcg
     1021 gagtggacga aggcgcgcgc cgcgtcatag ctctctccag ggacgagaat cccttcctcc
     1081 tgactttggt tctgatatgc agaaccaaac tcaggagttg gaccgccgca tcgcggcttt
     1141 ggcgcgtcgg cagcatggtg ttgttgctca ctggcagctg ttgacgcttg ggctggggaa
     1201 gacggctatt cattggcgag tgagcgcggg acgcctgcat cgcgtccacc gcggcgtgta
     1261 cgcggtcggt cacacggttc tcacacccaa cggacgactg atggctgcgg tgctcgcgtg
     1321 tggcgatggg gcggtgctga gccaccgaag cgccgccgac ctgtggggtc tacgccgttc
     1381 tctaagtgct gccgtagacg taacggtgca gagcagcggc cggcggaggc gcgaaggaat
     1441 ccgggttcat caagtcacgc gccttgacaa gcgggacgtc atgacccgtg acgggattcc
     1501 ggtcacaacc atcgcccgca cgctcctcga cctcgcggcc gtcctcaacg agcgtcaact
     1561 gacgtacgcg atcgaacagg ccgagcgcca gcagcgcttc gacaagaagg cgataacgga
     1621 ggcgtgcaag cgaaaccctc gccactccgg gctgagagct ctaagagctg ctgttcaggc
     1681 gttcgagccc gagctgaccc acacacggtc agacctggag cgcgaattcg tgtccttctg
     1741 ccgggactgg gacatcccgg ttccggcctt caacgttgtg gtgtgcggct acaccgtgga
     1801 cgccgcctgg caacgcgagc gcttgatcgt cgagctcgac acccgtgact accacgacga
     1861 cggcgcagga ttcgaagaag accgcaggcg ggacgccgaa ctacaacgca ggggctaccg
     1921 ggtaataagg atcaccgcga aacgattgcg agaggagccg caagccatcg cggagctact
     1981 gagagaactg ctcgctcagg cgaacgccgg cgcgcccgcc accactcggt tgcgcccctc
     2041 gagcttcgct cggtagagca gttcgtccgc gctgtggaag aggcgctcga agtcgagcgc
     2101 cagcccgcgc gccgagcaca cgccgaacga aatcgagatg tcgagcccgc cggggcgcgc
     2161 ctccgacacc gccttccgca tgcgctccgc gatcgccacc gcgtcgccct gcgaggcgcc
     2221 cggcagcagc accacgaact cctctccgcc gtagcggtac acgaggtcga acgagcgcag
     2281 cgccgtccgg aggatgtacg ccacgtcgcg cagcacgttg tcaccgacca ggtgtccgtg
     2341 ctcgtcgttg acctccttga agtggtcgag gtcgcccacg agaaccgcga tcggcgcatc
     2401 cgtcaggtgc gcctgctcca cgagctcgac cgcccggcgg tgcagcgcat ggcggttgaa
     2461 caggccagtc aaaggatcga gcgcggcttc cgcgcggtgg agcacatctg accgcatcag
     2521 cgccgtcgtc aggacggcga tgccgccgat cacgctcagc ggcacaacga ggatctgcgg
     2581 gttgtggagc acggcgctcg gctggcggct gagcgcgacg gcgacggcga tcgcggcggt
     2641 ccacgccacg ccgacgatca ttccgcgcgc ggtgaagcgc gcgctcagcg tcgcgacggg
     2701 gatcgccagc cacgggaaga agctcgatcc cgctccgccc tggatcacca cggccacgcc
     2761 gatcatcagc tggcagaacg cccaggcccc cgcaacccag tattccggac ggtcgctgtg
     2821 caccatcatc tggtcggcga tcacgaacac gatcaccgcc ccggccagcg gtccgagcgt
     2881 ccaccagccc acccacgggc tgagcaccat gagtgacaca gcgagcaggg cgaagaccgc
     2941 gcttcgcgcg ggcttgaggt ggcgctccat gtcgagcagc ctgcggcgcg cgaagtcgtc
     3001 cgcgcaaagc cagcttccct gccggcctgg gggcttcgag gatccggtgg cgaccattcg
     3061 gttagtcgcc tctttcatcg gcgcgcggcg cgaccgcata aagagtccgg tcaggacatc
     3121 gacaaacccg cagacttgcc gcacgggaca agtgaaaatc ggcgctgccc cactaggctg
     3181 cgcgtctatg accaccccca ccacccgtga gctcgaccgt ggccgcatcc gcgcgctcac
     3241 cgcccgggag ctcgacgcgc tcaacgaacg aacgcagaag tccggggaga cctatcgccg
     3301 cgccgagcag gtgctgtccg gcggagtcgc ctcctcctac cagctgcgtg agccgtggcc
     3361 gatctacctc gagtacgggg agggcgcacg cgtgtgggac gtggacggga acgagtactt
     3421 cgacttccac aacggcttcg gctcgatggt gcaggggcac gcgcaccccg cgatcggcgc
     3481 cgccgtgcag aagcgctacg cgcagggcac gcacttcgcg gcgcccaccg aggacgcgat
     3541 cgtggtgggg gaggagctcg ccaagcgctt ccggctcccg cgctggcgct acacgaactc
     3601 gggctccgag tcgacgatgg acgcgatccg catcgcccgc gggctcacgg gccgcgacga
     3661 cgtgctcaag atcttcggct cataccacgg ccatcacgac accgtgatgg tctcgatcgg
     3721 cgtggcgtac gaccagatcg gcccgccgga ccgcccgaac tcgctgccct acggcgccgg
     3781 cattccgcag tccacggttg accacgtgca tgccgtgcac ttcaacgacg ccgagacgat
     3841 ggaccgccgc atcgccgagc tcgacgccga ggggcgaaag cccgcctgcg tgctcatgga
     3901 ggcggcgatg atgaacctcg gggtcgtgct gcccgaggac ggctacctcg aggcggtgcg
     3961 cgagatcacg cggaagcacg gcgtggtctt gatcttcgac gaggtgaaga ccggcctcac
     4021 gatcgccgcc ggcggggcgg tcgagcgctt cggcgtcgag cccgacatgg tgacgctcgc
     4081 gaaggcgctc ggcggcggcc tccccacggg cgcgatcggc atgagcgaag aggtcgcccg
     4141 cgtggtcgag gacggcagcg tctaccaggt gggcacctac aacggcaacc cgctcggcat
     4201 ggccgccgcg cgcgcgaacc tccaggacgt gatgacgccg gacgcctacg cgcaccttca
     4261 ccacctgaac gaccgcatcc tcgcgagctg cgaggaggtg gtggaccggc acgggctgcc
     4321 cgcctacacc gtcggaatcg gctcgaaggg ctgcgtcacg ttcgcggccg agaaggtggt
     4381 ggactaccca agcttcaagc gctcgcagga tcccgagctg tcagagctcg cgtggctgtg
     4441 gtcgatgaac cgcggcatct tcatgacgcc gggccgcgag gaggagtgga cgctgtcggt
     4501 gacgcacacc gacgaggcca tcgaccgcta cgtgagcgtg ttcgccgagc tggccgccga
     4561 gctgaccgct taggagaaga ccggctcctc ccgcccgggg aagatctccg gcagtccgtc
     4621 gctcacgcga tcggtgaacc gcggcgggcg cttctcgagg aacgacgtga cgccctcgac
     4681 cgcgtcggcc gactggccgc gcgagaacat cgcgcgcgag tccgcgcggt gtgcgtccat
     4741 ggggtgcgag aaaccgagtc ccgcccacat cagccgccgc gccagcgcca ccgagacggg
     4801 cgccgcgttc tccgcgatct cggccgcgag cgcccgggcc tcagcgagta cctgatcggc
     4861 ggggtggagg ctgcggacga gccgcccggc gagcgcctcc tcggagctga acacccgccc
     4921 ggtcgcggcc cactccatcg cctgcgagat gccgacaatg cggggcagga accagctcga
     4981 gcacgcctcc ggcacgatgc cgcgccgcgt gaagacgaaa ccgatcttcg tgccctcggc
     5041 gacgagccgg atgtccatcg gcagcgtcat cgtgatgccg actcccacgg ccggcccgtt
     5101 gatcgcggcg atcaccggct tcgtgcactc gtggatgcgg agcacgactt gtccaccacc
     5161 gtcgcgcggc accgactcgc cctccgaacg cccacgccaa tcgaaggtcg cgccgccggc
     5221 ctcgaggtcc gcgcccgcgc agaagccgcg cccctcaccg gtcacgacga tcgcgcgcac
     5281 gtcgtcgtcg gcgtcggcgg cgtcgaacgc ggcgaccagc tcctcgccca tcttgcgcgt
     5341 gaacgcgttg agccgatccg gccggttgag ggtgatcgtg aggatgttgt cggccacctc
     5401 gtagcggatt tcctcgaagt ccatgtgcag ttccttccgt tccgggagcg ggcagcgcgc
     5461 ggcggcgctc agctgcgtat cctgccgccc cgcgggccca cgtgggcagg gcgaaccggc
     5521 ggcttctcga tcgggcccgt tgcgtccgaa gctgccaaag ggagcgacat gaacctcagg
     5581 accgcggtcg gcgcggccgc cgtcgtcacg gcgctgtgtg cgggagtcac ggcgcaggca
     5641 tcggcgagcc cgctcgcgca cgggcacttc gtgaagatcc aaaagcaccg cacgaaggcc
     5701 aacgccaacc agagcacgaa ctggttcggc tacaaccagg gcacgctcga gcagggcgga
     5761 aagcagttca actcgatcac cggcgactgg accgtgccga ctgcgagcca gcacaccaag
     5821 gaccaggacg cctactcctc agactggatc gggataggcg gcggctgcgt ggacgccaac
     5881 tgcacgattg gcgacagcac gctgatacag accggcacgg agcaggacgt ggacaccgcc
     5941 ggaaattcgt cttacagcgc ctggtgggag ctgatcccgg ggccgtcgat cgacatctcg
     6001 aacttcccgg tgtcgcccgg cgaccacatg aatgcggaga tcgccgagac ggtcgcgggc
     6061 gcgaacgtct ggaagatcac gctcaagaac gtgaccaagg ggcagacctt cacaacgacc
     6121 gttccctaca gctccacgca cgcgaccgct gagtggatcg aggagacgcc gctgctgatc
     6181 ggcacgagcc cgggcttggc gccgctgccg aacctcacga agacgccgtt ctcggcggga
     6241 accaccaacg gcaagaacgc cggtctgaag gcctcggagg agatccagct gatcgactcg
     6301 aatggtcacg tgatcggcac tccgtcggct ccgagcgcga ccctcgacgg cctcaacgtc
     6361 tgcgcctggg cgaccagctg cagctgatgc acacgcgagc gaggggcccc gcgacgcgcg
     6421 gggctcctcc gctcagcagc tgtgctcggc gaacaggcgc gcgctcgagc cgggctggat
     6481 cgccgagccg gcgcccggcg cggtggcgca gacggtgtag ttgctgtcga tgatcacgcc
     6541 gagcatgcct ccgccggaca ccgagtagtc gagtccgagg ctgtcgaggt agtcctcggc
     6601 cttgtcgagc tgcgcgccct cgatgtgcgg catggtcaca acgccgggaa cggcgggcgg
     6661 tgagtcggcc acgcacgcga acattggcgc cccgtcggcc tcgatccggc tccagctgcc
     6721 ggcgcgctcg ccgctcccgc aggtctggcc ctcgatcgga gtgatcacct tctcgccgtt
     6781 ctgcgtgcac tccgtggcgg cgcagctgac cgtgtccacc gggtttgccg cgggggtggg
     6841 ggcggggcgc ggggccgcag gcttggcggc acgctcgccc gcgtcgctgc tgccgccgcc
     6901 ggcgggcaga aggaggatgg cggccaagcc tgctgccgcg aacagcacca gcgcggcgag
     6961 ggcaggagcc acgcggcggc gccggcgcga cgtgacgagc ttcgtcgtgc gcgccgtcga
     7021 gggcccggac ggacgccggc gctgaaggag cgtgcgcgtc cgcccacccg cgatcgcggc
     7081 ctgcgccgcg cgcgccagct cgcccgccga gtgatggcga tcgcgcgagt ccttggcgag
     7141 cgcccgcgcg agcacgggct cgagtgcggc cggtagctcg gggcggcggt cgcgcaggcg
     7201 cggtgggcgc tcgttcatgt gcgcccagcc ctttcccgct cgcggcacgt cggcgaaggg
     7261 agcggcgccg gtcagtgccg catagagcac gcagccgagc gagtagacgt cgctcgcagg
     7321 acccagctcg cgcccctcgc cctgctcggg cgacgagtag tccggcgtgc cgagccagcc
     7381 acctgtcgac gtcagctggc ccgacgagcc gtcacgcttg gcgaggccga agtccgtgag
     7441 gtagacgtgc gggcgctcct ccccggcgat caggaggttc gccggcttca cgtcgcggtg
     7501 caccaggccg cgggcgtgcg cggcgtcgag cgccgacgcg gcctgcgaga tgagctcgac
     7561 ggcgagcgcc ggctcgagcg cgccgcgctc gcgcagcacg cgctgaaggt cggtcccctc
     7621 cacgaagcgc atcgcgatga acaggcgtcc atcgtcctcg cccgcgtcat agaccgggat
     7681 cacattgggg tggtcgaggc cggcggtcac ttgggactcc cgcgcgaagc gcgcgcggaa
     7741 cgtcgagtcg cttgcgagct cgggggcaat gaccttcagc gccacaaccc ggtcgagttg
     7801 agggtcgcgg gcccggtaga ccacgcccat cccaccgcgg ccagcctcgg cctcgatgcg
     7861 gtagcgcccg actttcatgc cgagcggcgg tgcgagtgcc actcccgggt tatagcgaac
     7921 accggaggag acaacgtgta aattcgctct cggaaggcgc cgtgaaggat ctcctgctgc
     7981 accgcccggc ttggtacgtc ctgggcccgc tgctcgggct cgtcgtgctc ggcctctacg
     8041 ccgcgatcag cgagcgcttc ggcgtcgtgg gtggctattc gaacgtggtc gagcgcatca
     8101 gcggccgcct gccgaagctc ggctggaagg cgtgggtgct gttcggagtg ctcggcggga
     8161 gcctcgtgta ccggctcgct gcaggtcgtt cgaacattgg cgacggctac ggctggctca
     8221 cgcgcacgtt cggtgggagt ggcgggcacc tgatcgtggc agggcttctc gtcgccgccg
     8281 gcgtgctgat cggctttggc gcgaagacgg ccggtggctg cacgtccggt aacggtcttg
     8341 gcggctgctc cgccgggtcg acctcgagct tcgccgcgac cgcgacgttc atgggcacgg
     8401 cgatcgtctg ctcgttcctg atcaaggcga tcacatgact cgcaccaagg cgacagcagc
     8461 gctgttcggg gccgccttcg gcttcctcat ctcatggggc cagttcgccg acccggaccg
     8521 catccgcgac atgctgctcc tgcgcgatcc ctacctgtac ctgatgatgg cgagcgcggt
     8581 ggccgtcggc ttcgtgggcc tccggctcct gacgcggcgg ggcgcccggg cgtttctcac
     8641 cggccggccg atcacatacg agcgcacgcg tccacagccc cgccacttcg cgggcgccgc
     8701 gatcttcggc gtgggctgga gcatcgccga ctcgtgcccc gcgccgatcg ccgcgcagct
     8761 gactcagggc gtcgtgtgga gcgtgttcac gatcgccggc atctggctcg gcatcctgct
     8821 cttcctcaag cgccaggagc ggcgcgagca ggtacccgag cgccgcgcgc tcggcggact
     8881 ggctgtggac aagccaccgg gctgaagcgt cggggtaata gcatcgggcc ttcccgagga
     8941 ctcgcccgag gatgagtaca gacactgcct ctacgcgcgc ggcagagcgc gacctgctcc
     9001 ggacaatcgc tgcgggtacg gccaccgtga ccgggcgcgc gttcttccgc tcgctcgtac
     9061 accacgtcgc actcgcgctc ggtgccgacg tggcctgggc ttccgagatg caagccgggc
     9121 cgggggagcg cgcggtcgtg atcgcgtcct gggggcgtga ggacatccgg ctgcccgagg
     9181 gacacgatta cgacctgtcg cagacgcctt gcgaggggac gatcgagcgc gacgtcgtct
     9241 cgatcccgga gaacgtcggc gaggcgtacc ccaacgacac gttcatcgcg gaacacggcc
     9301 tctcgggcta cctggccgtt gtgatgagaa gctcggacga cgagcggctc ggctatatcg
     9361 gcgtgatgtc gcgggggcga ctcgaggcca ccgaggacga gctcgcggtg ctgtcgatct
     9421 tcgcctcgcg agcggccgcg gagatcgagc ggcgacgcca cgaggtggcg ctgcgtgcgc
     9481 gtgaagccga ggtcgctcag tcgcggtcac gcgtggtcag cgcggccgac gaggagc
//
LOCUS       DASVXS010000068        14370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1069340, whole genome shotgun sequence.
ACCESSION   DASVXS010000068 DASVXS010000000
VERSION     DASVXS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069340"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..535)
                     /locus_tag="VF752_03785"
     CDS             complement(179..535)
                     /locus_tag="VF752_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8854698.1"
                     /translation="MGRALAAAVSLVVAGTLVLALAAGAGGASRKTVRLGDNYYTPPK
                     LTVSKGTTIVWKWPSDTGDTHDVTLGKRPKGVKKFQSELASIDYSYKRTLKVPGRYYI
                     YCSLHDDMRQTITVKR"
     gene            complement(520..1827)
                     /locus_tag="VF752_03790"
     CDS             complement(520..1827)
                     /locus_tag="VF752_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8854699.1"
                     /translation="MRAKLAIAAGLGLALASAAPAMAVDYPPPKNPGGVSKPPTGPHH
                     TLRVGKHEQYKTIQSAVKAAKAGDMIRVAHGTYREGVKITGSAKRYLKLIGDPRHPDK
                     VVIEAKKAQNGVFINGANQITVQGFQAQHYKANGFFAVNVTGYKFDRLIATDGGVYGV
                     YAFNSKGGTISNSTASWNNDSGFYIGQTPRQTKPIRSVVKNITSYGNVLGWSGTNMRY
                     VTITKSRFFNNGTGIVPNALDSEKFPPPEDNVISNNEVFWNNFDYYKGAPFKLRPPAT
                     GDVPYPTGVGILLFGGRGNTIQSNRVYGNWGVGVGMLQQFLLKQKDAQDLKNNQVKNN
                     QFGLNGTDLNGRDLFYDGNGSGNCFSGNTGVQVTLPEDGSTFVNCPFSGNNTFNQQAQ
                     TTAIGFAVAPDQETSWIQHPHAAKKGYNALVRWKKGKDIWGGP"
     gene            complement(1839..2906)
                     /locus_tag="VF752_03795"
     CDS             complement(1839..2906)
                     /locus_tag="VF752_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX8854700.1"
                     /translation="MSDEGGFERRSFLASAGSLAFVCGLGGQPLAASTASDVLKADTA
                     ARAIKRPKTVAKDPVDSLTFETPQPQPGGQAREYWVLARSVTWDPCPTGRDEWMKHPV
                     PGRRKFRAFVYQRYTAGFAQPIGPPQMPGPTLEAEVGDTIVVHFRNGDRHFGQAVTMH
                     PHGVRYTPDYDGAYYGDFTRAGGFVAPGEEFTYTWEATPDSVGVWPYHDHGPNHVINL
                     SRGLFGAVIVREKGARKPDVEQVLFMHALLPQITGNRVPLQCFNGRAFAGNTPTIRAK
                     VGSDVALHVFGADGNFHTFHVHGHRWKDSGGADVDSPTVGPNESLTARWTEDNPGRWL
                     YHCHVFAHQDAGMAGWYLVDP"
     gene            3021..3644
                     /locus_tag="VF752_03800"
     CDS             3021..3644
                     /locus_tag="VF752_03800"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8854701.1"
                     /translation="MAGSDRQPQPNGTTDRRDAILRAALQLIGRQGLHAVTHRDVAAE
                     AGVPLASTTYYFESKEELLTEALLLFVRDELERLEQAAASFEGVSTSPEQVADAMTAE
                     VQRTLDRPELQVAQFELYLQATRDPQLARASVQAFDAYMKTTKAALRAAGAENAEELA
                     PLFQALARGLVFAQLVDRQPDWAEKILKPALLRLFRALSPQAERQLP"
     gene            complement(3641..4384)
                     /locus_tag="VF752_03805"
     CDS             complement(3641..4384)
                     /locus_tag="VF752_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX8854702.1"
                     /translation="MSLPQLVSLDEWLAARKRLLEKEKELTRARDALSAERRRLPMVR
                     IEKDYRFEGPGGQASLRDLFGDHRQLIVRHFMFDPSWENGCPSCSAAADELSAGLLVH
                     LRARDTEFVTVSRAPIEKIADYKARRGWDFPWYSSYGSDFNYDFAVTLDESVAPLEYN
                     YRSKAEHDAAGTGYYLEGEQPIELPGHSCFLRVGDEVFHTYSVYARGAEMLGGSYYFL
                     DMTALGRQEDWEEPKGRAAAVREARPDFA"
     gene            complement(4486..5022)
                     /locus_tag="VF752_03810"
     CDS             complement(4486..5022)
                     /locus_tag="VF752_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8854703.1"
                     /translation="MSEYVLRPSDGRYVQLGGLGVRHMIESDRTGGAFALVEHPIAPR
                     ALAAPTHTHTHEDEYSYVLEGRVGVQIGDEVLVAGPGELVFKPRGIPHAFWNAGDEPA
                     RLLELIAPAAFAAYFDEIAPALPPTVREPDLQRIAEIQARYGLTMDMDSIGPLVERHG
                     LVMEALPPSTSADYSRTV"
     gene            5113..5718
                     /locus_tag="VF752_03815"
     CDS             5113..5718
                     /locus_tag="VF752_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8854704.1"
                     /translation="MTSRSYKLKQRAESMADTRRRITEATAALHQEVGPARTTVSEIA
                     RRAGVGRLTVYNHFPDERELFAACQAHYLAENPPPDLSELAAERDPDARLTQVLEALY
                     GYYRSTQLMTGNVDRDVRAMPVLREVVEAWRAPMFDALTGALMRGRALRGKRAARTRA
                     AITLALEFRTWDTLASRGGLTDADAAGVAAGAVRAAAGPAS"
     gene            complement(5744..7654)
                     /locus_tag="VF752_03820"
     CDS             complement(5744..7654)
                     /locus_tag="VF752_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX8854705.1"
                     /translation="MDPEARRALTKSAVVVGALGVVFGDIGTSPIYTIQTAFNPGDPH
                     PIQTSLQSVYGVVSLIFWSVTLIVTVKYVGLVMSADNEGEGGIMALISQIREIGKEVP
                     ARTKFVLASVGVFGAALFFGDSMITPAISVLSAVEGTKSISSSMDSFVIPITLAIVVV
                     LFAIQRYGTGSVGRLFGPIMLAWFATIAVLGVRGISMRPEVLRALSPSYAFDFLFHSG
                     STGFFSLASVVLAITGVEALYADMGHFGRPAITRAWLALVFPACILSYLGQGALILDD
                     PDAIGAPFFRLMPHGGLVPLVVLATAATVIASQAVISGAFSIAHQAAQLGYLPRLRVI
                     HTSERTYGQVYVPFINWLLMGAVVALVLAFQSSQKLAFAFGMAVTGTIMITTILFFVI
                     VRRRWGHPLWLVVLGAVPFLAVELAFFAANLTKFTHGAWLPLLIGVIVFTVMTTWYRG
                     RELVTAERFRIEGPLQAFVDGLRNMDPPLTRTRGTGVFMNRGKETAPLSMRACVDHLD
                     ALQEHAVILSLETLPTPRIKPADRLEIDDLRYRDDGITFVRAKHGYAEHYDVPTLVTQ
                     IAEADIESRVDLRGTSFYLSRVELKATDRPGMSRWRKRLFLVTALISAEPADYFQLPR
                     DRTVTLGAEVEF"
     gene            7881..9365
                     /locus_tag="VF752_03825"
     CDS             7881..9365
                     /locus_tag="VF752_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8854706.1"
                     /translation="MFYATYVLSELRRRKGRTLLTALGLAVGVGLVVTVSALSKGLDN
                     AQSKVLEPLTGVGTDMSVSRPLKVSGSGNSQTFGPGGGPRLSPNEQKQLRDENGGARL
                     GLRNVAKPGERFSRDDFISTSQLSFSESEVAKIAALDGVQQASGALTLSSVHVSGRVP
                     KDAGSNSGPRVFAQPGPAAGAGSAGPKAINFDARTVTGVDVSKQAALGGQITKGRYLA
                     SSGKKYDAVLNESYAKRKGLSVGDTFTLSGKKFTIVGLMSAPIGGQASDIYMRLDVLQ
                     KLSGREGRVNTVQVRAASTDAVGRVKSEIQRTFAGAEVTTAKDLADRVGGSLVDAKNL
                     SNKLGTALEVVGLAAAFLIASLLTLSSVAKRTRELGTLKALGWRQRLVVRQVTGEALA
                     QGALGGIVGVLLGICGAALLNAFGPTLKATVAAAQPSGPGPLPFGQGQVTSGSTTVAL
                     HAPVDAGLILLAVALALLGGLLAGSVGGLRAARLRPAEALRSVE"
     gene            9362..10087
                     /locus_tag="VF752_03830"
     CDS             9362..10087
                     /locus_tag="VF752_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854707.1"
                     /translation="MTNYETRNQVLYQVEGVVKRYGEDLTEVRALDGVTLTIDEGEFV
                     VVAGPSGSGKSTLLQLLGGLDRPTSGRVVFEGRDLATMGDGELAELRLRTLGFIFQQF
                     NLIPTLSARENVEVALAPRGIPAAQRRETAVRLLDQVALSGRVDHLPSQLSGGEQQRV
                     AVARALSNEPRVLLADEPTGNLDSATGDDLFALLRRLNDEGQTIVLITHDRSIAERAP
                     RRIEMRDGRLQDGLQPLAGASAG"
     gene            10097..10777
                     /locus_tag="VF752_03835"
     CDS             10097..10777
                     /locus_tag="VF752_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854708.1"
                     /translation="MTRVLVVDDERSVRDAIRRALTLEGYEVDLADGGLSALSHLTAR
                     PDAMVLDVLMPDLDGLEVCKRVRSAGDRTPILMLTARDGVSDRVAGLDAGADDYLVKP
                     FALEELLARLRALLRRSLPDGDGDVLVFSDLMLDTAAHEARRGERRIDLTRTEHQLLE
                     LFMRNPRRVLTRSQINERVWGYDFGPASNSLGVYVGYLRRKLEEGGEPRLIHTVRGVG
                     YALREEDR"
     gene            10774..12192
                     /locus_tag="VF752_03840"
     CDS             10774..12192
                     /locus_tag="VF752_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8854709.1"
                     /translation="MTLRNRLAVAAAVAVALAVAGGCIASYLAVRAKMRDEVDTSLRQ
                     RANELSSLAQNGPGQMDDEPFSKPAPAPRFGGAPGYAQVVAPDGDVLLPPGERTQLPV
                     AGEARRVTQGNNEPVLRDVRVNGAHLRVITAPLREGFAIQIARPLSEVDSVLHSLVIV
                     FLFITAAGVGLAVVLGRLVARTALAPVERFTAQTEQIAEHPDEGRRIDVEGEDELARL
                     AASFNATLDALEHSLASQRQLVADASHELRTPMASVRTNIEVLAKGDRLDPTERERLV
                     SDIIEQIDELTALVADVVELARGAKPDEAKEQVRLDELAAACTDRARRHAPDVTFETR
                     LEPVAVTGNPQRLHRAISNLLDNAAKWSPPGGVVEVEVRDGSVTVRDHGPGIKPQDLP
                     HVFDRFYRAPDARSMPGSGLGLAIVKQVAESHGGTATAMNAEDGGALLRLSFPSWTRP
                     STSEGPALGASVQQPQSQVRHE"
     gene            complement(12297..12575)
                     /locus_tag="VF752_03845"
     CDS             complement(12297..12575)
                     /locus_tag="VF752_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX8854710.1"
                     /translation="MSCTHLDMIKLEAVPAEVAGCEECLKTGDRWVHLRTCLTCGHVG
                     CCDSSPNRHASKHAAEVSHPIVRSAEPGEDWSWCYVDEEAFIVDIPVV"
     gene            complement(12594..13130)
                     /locus_tag="VF752_03850"
     CDS             complement(12594..13130)
                     /locus_tag="VF752_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8854711.1"
                     /translation="MTDLAEAVGPRDHSRGAEGATTLLVYGDYECPYTRAAYRSVEVL
                     ERRLDGAFRFVFRHFPLTEVHPHALAAAQAAETAAALGSFWEMHELLFEHQKALERED
                     LEGYASTLGLDLAAFRAGIDSSEYLPGIEHDVRTGIDSGVRGTPTIFLDGEVYDGSYM
                     AAALQPEIERRLAAGRGA"
     gene            13214..13840
                     /locus_tag="VF752_03855"
     CDS             13214..13840
                     /locus_tag="VF752_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8854712.1"
                     /translation="MNIGIIGSGRIGATVAWLLTRAGHEVALANSRGPDTLAEHVGKL
                     GPRAHATTAAKAATFGDVVVVAVPVKAFPDLPAEQLSGKVVVDTGNYYPQRDGNIEEL
                     DDDTTTSTELLGRQLSGARLVKAFNTMYWENLRDDGRPDAPRDDRLAVFLAGDDEDAK
                     KIVSGLIEEIGFAPVDTGSLAEGGRRQQPGSPIYGSLLKAPEAEAALA"
     gene            complement(13837..>14370)
                     /locus_tag="VF752_03860"
     CDS             complement(13837..>14370)
                     /locus_tag="VF752_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX8854713.1"
                     /translation="SGLSGLASRVEALGGTLRVDSPPGEGTRLEVSIPLSPWRDAREP
                     FLEFGHEDDGGLGERLIDKVISGEKTAVISLAREWDLEGGPPRMGQRLPVMDHTGRRR
                     ATIEVVRMAGVPFGEITKDIVAAQTGSSEPLESWRAVQREYYDGCRDEIAVLLGEPGW
                     RLTEDEPMIIVWFRVAD"
BASE COUNT         2278 a         4878 c         4933 g         2281 t
ORIGIN      
        1 gacgcttggt cacttacggg gggagcgccg tcacttaggg gccactatgg cccctgatcg
       61 accacaccga gggagccagg ggcgagcgtc gtcactcagg gtgaatagtt caccgcgagc
      121 gacgacagcc gctcaacccc acaccgggcg ccctaaatgc taagccgtca agcgacccct
      181 accgcttgac cgtgatggtc tgcctcatgt cgtcatgcag cgagcagtag atgtagtacc
      241 tgcccggcac cttcagcgtc cgcttgtacg agtagtcgat cgacgcgagc tccgactgga
      301 acttcttcac gcccttcgga cgcttgccga gggtcacgtc gtgcgtgtcc cccgtgtcgc
      361 tcggccactt ccaaacgatc gtcgtgccct tcgagaccgt cagcttcgga ggggtgtagt
      421 agttgtcgcc gagtctcacc gtcttgcgcg aggcgccgcc cgccccggcc gccaaggcca
      481 ggacgagcgt gcccgcgacg acgagtgaga cggcggccgc tagggcccgc cccatatgtc
      541 cttccccttc ttccaccgca cgagcgcgtt gtagcccttc ttggcggcgt gcggatgctg
      601 aatccacgac gtctcctggt cgggagcgac cgcgaagccg atcgccgtcg tctgcgcctg
      661 ctggttgaac gtgttgttgc cgctgaacgg gcagttcacg aacgtggagc cgtcctcggg
      721 cagggtcacc tgcaccccgg tgttgccgga gaagcagttg cccgagccgt tgccgtcgta
      781 gaacaggtca cgcccgttga ggtccgtgcc gttcaggccg aactggttgt tcttgacctg
      841 gttgttcttc aggtcctgcg cgtccttctg cttgaggaga aactgctgca gcatcccgac
      901 gcccacgccc cagttcccgt acacgcggtt gctctggatc gtgttaccgc gtccgccgaa
      961 caacaggatc cccacgccgg tcgggtacgg cacgtcgccg gtcgccggcg ggcggagctt
     1021 gaacggcgcg cccttgtagt agtcgaagtt gttccagaag acctcgttgt tcgagatcac
     1081 gttgtcttcc ggcggcggga acttctcgga gtcgagggcg ttcgggacga tgcccgtgcc
     1141 gttgttgaag aagcgcgact tcgtgatcgt cacgtagcgc atgttcgtgc cggaccagcc
     1201 gagcacgttg ccgtacgagg tgatgttctt cacgaccgag cggatcggct tcgtctggcg
     1261 cggcgtctgc ccgatgtaga agccgctgtc gttgttccac gacgccgtgc tgttcgagat
     1321 cgtgccgccc ttcgagttga aggcgtagac gccgtagacg ccgccgtccg tggcgatcag
     1381 gcggtcgaac ttgtagccgg tcacgttcac cgcgaagaag ccgttcgcct tgtagtgctg
     1441 ggcctggaag ccctgcaccg tgatctggtt ggctccgttg atgaacactc cgttctgggc
     1501 tttcttggcc tcgatcacga ccttgtccgg gtgccggggg tcgccgatca gcttcaggta
     1561 gcgcttggcg gagccggtga tcttcacgcc ttcgcggtag gtgccgtggg ccacgcgaat
     1621 catgtcgccg gccttggcgg ccttcaccgc cgactgaatg gtcttgtact gctcgtgctt
     1681 gccgactcgg agcgtgtggt gcggccccgt aggcggcttg ctcaccccgc ccgggttctt
     1741 cggcggtggg tagtccacgg ccatcgcggg cgctgcgctc gcgagcgcga ggcccagtcc
     1801 agcggcgatt gccagctttg ctctcaaatc ccctcctctc agggatcgac caggtaccag
     1861 cctgccattc ccgcgtcctg gtgtgcgaac acatggcagt ggtagagcca ccgcccggga
     1921 ttgtcttcgg tccagcgagc cgtgagcgat tcgttcggac ctacggtcgg ggagtccacg
     1981 tccgccccgc cgctgtcctt ccagcggtgg ccgtgcacgt ggaaagtgtg gaagtttccg
     2041 tcggcgccga acacgtgcag cgccacgtcg gagcccacct tcgcgcggat cgtgggcgtg
     2101 ttgccggcga atgcgcgccc gttgaagcac tgcagcggca cgcggttgcc ggtgatctgc
     2161 gggagcagcg cgtgcatgaa cagcacctgc tcgacgtcgg gcttgcgggc gcccttctcg
     2221 cgcacgatca ccgctccgaa caggccgcgc gagaggttga tcacgtggtt cgggccgtgg
     2281 tcgtggtagg gccagacgcc gacagagtcg ggcgtcgcct cccaggtgta ggtgaactcc
     2341 tccccgggag ccacgaagcc gccggcgcgc gtgaagtcgc cgtagtaggc gccgtcgtag
     2401 tccggcgtgt aacgcacgcc atgcgggtgc atggtcacgg cctggccgaa gtgccggtcg
     2461 ccgttgcgga agtgcacgac gatcgtgtcg cccacctcgg cctcgagtgt tggcccgggc
     2521 atctgaggcg gcccgatcgg ctgcgcaaag ccggccgtgt agcgctggta gacgaacgcg
     2581 cggaatttgc gcctcccggg taccgggtgc ttcatccatt cgtcgcggcc cgtggggcat
     2641 gggtcccagg taaccgagcg ggcgaggacc cagtactcgc gcgcttgccc gccgggttgg
     2701 ggctgcgggg tttcgaacgt gagcgagtcg accgggtcct tcgccaccgt cttcggccgc
     2761 ttgattgccc gggcggcggt gtccgccttc agcacatcgg aggccgtgct cgccgcaagc
     2821 ggctgtccgc caaggccgca gacgaacgcc agcgagcccg cgctggccag gaagctgcgg
     2881 cgttcgaatc cgccctcgtc gctcatccaa agagtcccgc gccgggtgac gggggagtct
     2941 tgtccccggc gcgggcttgt acatttgtac cagaaggctg tacaggtgta ccaatccctt
     3001 tagattccgc ggtgaggcgg atggcgggtt cggataggca gccccagccg aacggcacga
     3061 ccgacaggcg cgacgcgatc ctgcgcgcgg cgcttcagct gatcgggcgg cagggtctgc
     3121 acgccgtcac gcaccgcgac gtggccgccg aggccggcgt cccgctcgcc tccaccacct
     3181 actacttcga gagcaaggag gagctcctca ccgaggcgct cctgctgttc gtccgcgacg
     3241 agctcgagcg cctcgagcag gctgccgcct cgttcgaggg cgtgagcacc tccccggaac
     3301 aggtcgctga cgcgatgacc gccgaggtgc agagaacgct cgaccggccc gagctgcagg
     3361 tggcccagtt cgagctctac cttcaggcga cccgcgaccc gcagctggcg agggcgtcgg
     3421 tacaggcgtt cgacgcctat atgaagacca ccaaggcggc gctgcgcgcg gccggcgccg
     3481 agaacgcaga ggagcttgcg ccgctgttcc aggcgctcgc ccgcggactc gtgttcgcgc
     3541 agctcgtgga tcgccagccc gactgggcgg agaagatcct caaacccgcg ctgcttcggc
     3601 tcttcagggc gctgtcgcca caggccgagc gccagctccc ctaggcgaag tccggtcgcg
     3661 cctcacgcac cgcggcggcg cgccccttcg gctcctccca gtcctcctgg cggccgagtg
     3721 cggtcatgtc gaggaagtag tacgagccgc cgagcatctc ggcgccgcgc gcgtacacgg
     3781 agtacgtgtg gaagacctcg tcgcccacgc gcaggaagca actgtgcccc ggcagctcga
     3841 tcggttgctc gccttcgagg tagtagccgg tgcccgcggc gtcgtgctcg gccttgctcc
     3901 ggtagttgta ctcgagcggt gcaaccgact cgtcgagcgt cacggcgaag tcgtagttga
     3961 agtcgctgcc gtacgacgag taccagggaa agtcccatcc cctgcgcgcc ttgtagtcgg
     4021 cgatcttctc gatcggcgca cgcgagacgg tcacgaactc ggtgtcgcgc gcgcgcaggt
     4081 gcacgagcag gccggccgag agctcgtccg cggcggccga gcagctcggg cagccgttct
     4141 cccaggaggg atcgaacatg aagtggcgca cgatcagctg tctgtgatcg ccgaacaggt
     4201 cccgcaggct cgcttgtccg ccgggaccct cgaagcggta gtccttctcg attctcacca
     4261 tcggcagcct gcgacgctcg gcactgagcg cgtcgcgcgc tcgcgtcagc tccttctcct
     4321 tctcgagcaa ccgcttgcgc gcggccagcc attcgtctag cgagacgagc tgcggaagac
     4381 tcatcgttca cctccgggga gttcgagact gtctcgttac ccgggacgag gcggggacgc
     4441 gaaactcatc ggtcgcgccc ccgctgaggt ctccgctgtg tcgaatcaga cggtgcgcga
     4501 gtagtccgca ctcgtgctcg ggggcagggc ctccatgacg aggccgtgcc gctcgacgag
     4561 cggaccgatc gagtccatgt ccatcgtcag cccgtagcga gcctggatct ccgcgatccg
     4621 ctggaggtcg ggctcacgca cggtcggcgg gagtgccggc gcgatctcat cgaagtacgc
     4681 agcgaaagcc gcgggcgcga tcagctcgag cagacgagcc ggctcgtcgc ccgcgttcca
     4741 gaacgcgtgc gggatcccgc gcggcttgaa cacgagctcg cctggcccgg ccactagcac
     4801 ctcgtcaccg atctggactc cgacgcgacc ctcgagcacg tatgagtact cgtcctcgtg
     4861 cgtgtgggtg tgcgtcggcg ctgcaagggc acggggcgcg atcggatgct cgacgagcgc
     4921 gaaggcgccg ccggtgcggt cgctttcgat catgtgccgc actccgagac cgcccagctg
     4981 gacgtagcga ccgtccgagg ggcgaagcac gtactcactc atggctggct cctgtatttc
     5041 ggttgaacat ggtgttcagc gaatatacgc tccattggag gctgagtcaa gtttcgctat
     5101 acactgtgtt cagtgacttc acgcagctac aagctcaagc agcgggccga gtcgatggcc
     5161 gacactcgac gccgcatcac cgaggcgacc gcggcgcttc accaggaggt cggcccggcg
     5221 cgaacgacgg tgagcgagat agcgcgccgc gccggcgttg ggcggctgac ggtctacaac
     5281 cacttcccgg acgagcgcga gctcttcgcg gcctgtcagg cgcactactt ggcggagaat
     5341 ccgccgccgg acctctcgga gctcgccgcc gagcgggacc cggacgcgcg cctgacacag
     5401 gtgctcgagg cgctctatgg ctattaccgc tccacccagc tgatgacggg gaacgtggac
     5461 cgcgacgtgc gcgcgatgcc ggtcctgcgc gaggtggtcg aggcgtggcg ggcgccgatg
     5521 ttcgacgcgc tcaccggcgc gctgatgcgc gggcgagcgc ttcgcgggaa gcgagcggcg
     5581 cgcacgcgcg ccgcgatcac cctggcgctc gagttccgca cctgggacac gctcgcctcg
     5641 cgcgggggcc tcaccgacgc cgacgccgcg ggggtcgccg ccggcgccgt ccgcgccgca
     5701 gcaggcccgg cgtcgtaggt aatcgcctcc gccggtgagt ccctcagaac tcgacctcgg
     5761 cgccgagcgt gaccgtccgg tcgcgaggga gctgaaagta gtccgcaggc tcggcggaga
     5821 tcaatgccgt cacgagaaag aggcgcttcc gccaccggct catcccagga cggtcggtgg
     5881 ccttcagctc caccctgctg aggtagaaag aagtgcctcg taagtcgaca cggctctcga
     5941 tgtcagcctc cgcgatctgc gtgacgaggg tgggcacgtc gtagtgctcg gcgtagccgt
     6001 gcttcgcacg gacgaacgtg atgccgtcat ctctgtagcg cagatcgtct atctcgagcc
     6061 ggtccgcggg cttgatccgc ggggtcggca gggtctcgag cgaaaggatg accgcgtgtt
     6121 cctgcagcgc gtccaggtga tcgacgcaag cgcgcatcga gagcggcgca gtctccttcc
     6181 cccggttcat gaacacgcct gtgccgcgcg ttctggtcag cggcgggtcc atgttccgga
     6241 gtccgtcgac gaaggcctgt agcggtccct caatccggaa gcgctcggcg gtgacgagct
     6301 cgcgcccgcg gtaccaggtc gtcatgacgg tgaaaacgat caccccgatt aggagcggaa
     6361 gccaggcgcc gtgggtgaac ttcgtcaggt ttgccgcgaa gaaggcgagc tccacggcga
     6421 ggaagggcac cgcccccaga accaccagcc agagcggatg cccccagcgg cgacggacga
     6481 tgacgaagaa gaggatcgtc gtgatcatga tcgtgccggt cacggccatc ccaaaggcga
     6541 acgcgagctt ttgagagctc tggaaggcga ggaccagtgc cacaacggct cccatcaaga
     6601 gccaattgat gaacggtacg tagacctgcc cgtatgtgcg ctcggaggta tggatcacac
     6661 gcaggcgtgg caggtagccg agctgggccg cctggtgggc gatcgagaag gcgcccgaga
     6721 tcaccgcctg cgacgcgatg acggtggccg cggtggccag gaccacgagg gggaccaggc
     6781 cgccatgcgg catcagccgg aagaacggcg caccgatcgc atcggggtcg tcgaggatga
     6841 gcgccccctg acccaggtag ctgaggatgc atgccggaaa gaccaacgca agccaggcgc
     6901 gcgtgatcgc ggggcgcccg aaatgaccca tgtctgcata gagcgcctcg accccggtga
     6961 tcgcgagcac cacggaggcg agggagaaga agccggtcga cccgctgtgg aaaaggaagt
     7021 cgaaggcata actcggcgac agtgcccgga gcacctcggg gcgcattgat attccgcgaa
     7081 ccccgagcac ggcgatcgtc gcgaaccacg cgagcatgat cggcccgaac aggcggccga
     7141 cagagccggt gccatagcgc tggattgcga agaggaccac gacgatcgcg agcgttatgg
     7201 ggatcacgaa gctgtccatc gaggacgaga tcgacttcgt tccctccacc gccgagagca
     7261 ccgagatcgc cggtgtgatc atgctgtcgc cgaagaacag ggccgctcca aaaacgccga
     7321 cgctcgcgag cacgaatttg gtgcgtgccg ggacctcttt cccgatctcg cgaatctgcg
     7381 agatcagcgc catgatcccg ccctcgcctt cgttgtcggc gctcatcacc aggccgacgt
     7441 acttgacggt gacgatcaag gtcaccgacc agaagatcag ggagacgact ccgtacacgc
     7501 tctggagcga cgtctggatc ggatgcggat cgcctgggtt gaacgcggtc tggatggtgt
     7561 agatggggct tgtgccgatg tcgccaaaga cgacaccgag cgcgccgacg acgaccgccg
     7621 acttggtcag agcgcgcctt gcctcggggt ccatgtggca agcgtatgcc cgtcgtgcct
     7681 cttccccttg ccgcgggatg tgcggactgc agctgtgatg acaggcccgc gatcgagggg
     7741 ccgaacgggt tcttcgaaat ttcttacgcc gctctcccgc gcgtcttaga agcgcgctgt
     7801 tggctgttac tgcgctcagc ggcacgccgt ctcaagctct gccgctgagc gtcaaatctt
     7861 cagaccgaca ggagagccgc ttgttctacg ccacctacgt cctctcggag ctcaggcgcc
     7921 gcaagggtcg cacgcttcta accgcactgg gcctcgcggt gggcgtcggc ctcgtcgtca
     7981 ccgtgagcgc gctttccaaa ggcctcgaca acgcgcagtc gaaggtgctc gagccgctca
     8041 ccggcgtcgg taccgacatg tcggtttcgc ggccgctcaa ggtgagcgga agcggaaaca
     8101 gccagacgtt cgggcccggg ggcggtccgc ggctctcacc gaacgagcag aagcagctcc
     8161 gcgacgagaa cggcggcgcg cgcctcggtc tgcgcaacgt ggccaagccg ggggagcgct
     8221 tcagccgcga cgacttcatc tccacgtcgc agctcagctt ctcggagtcc gaggtggcga
     8281 agatcgcggc tctcgacggc gttcagcagg cgagcggggc gctcacgctc tcgagcgtgc
     8341 acgtgtccgg gcgggtgccg aaggacgccg gctcgaacag cggcccccgg gtcttcgcgc
     8401 agcccggccc cgccgcgggc gccggatccg ctggacctaa ggcaatcaac ttcgacgccc
     8461 gcacggtgac cggcgtggac gtgtcgaagc aggcggcgct cggcggacag atcaccaagg
     8521 gccggtacct cgcgagcagc ggcaagaagt acgacgccgt gctgaacgag tcctacgcca
     8581 agcgcaaggg gctctcggtc ggcgacacgt tcacgctcag cggcaagaag ttcaccatcg
     8641 tcgggctgat gagcgcgccg atcggcggcc aggcctccga catctacatg cgcctcgacg
     8701 tgctccagaa gctgagcggg cgtgaggggc gcgtgaacac cgtgcaggtg cgcgcggcga
     8761 gcaccgacgc tgtgggccgc gtgaagagcg agatccagcg gacgttcgcc ggcgccgagg
     8821 tgacaaccgc gaaggacctc gccgaccgcg tcggcggctc gctcgtcgac gcgaagaacc
     8881 tgtcgaacaa gctcggcacg gcgctcgagg tggtcgggtt ggccgccgcg ttcctgatcg
     8941 cgtcgcttct caccctgtcg tcggtagcca agcgcacacg cgaactcggc acgctgaagg
     9001 cgctcggctg gcgccagcgc ctggtcgtgc gccaggtgac cggggaggcg ctcgcgcagg
     9061 gcgcgctcgg cggcatcgtc ggcgtgctgc tcggcatctg cggcgcggcg cttttgaacg
     9121 cgttcggccc cactctcaaa gccaccgttg cggccgcgca gccgagcggg cccggaccgc
     9181 tgccgttcgg ccaggggcag gtcacgagcg gctcgacgac ggtcgcgctg cacgcgccgg
     9241 tcgatgcggg gctgatcctg ctggccgtcg ccctggcgct gctcggcggc cttctcgccg
     9301 gctcggtcgg cggccttcgc gccgcgcggc tgcgtccggc cgaggccctc aggagcgttg
     9361 aatgaccaac tacgaaacga ggaaccaggt gctctaccaa gtggaaggcg tggtcaagcg
     9421 gtatggcgag gacctcaccg aggtgcgcgc gctcgacggt gtgacgctca cgatcgacga
     9481 gggcgagttc gtggtcgtcg ccggaccgag cggctcgggc aagtccacgc tcctgcagct
     9541 gctcggcggc ctcgatcggc cgacctccgg ccgcgtggtg ttcgaggggc gcgatctcgc
     9601 cacgatgggc gacggcgagc tcgctgagct acgcctacgc acgctcggct tcatcttcca
     9661 gcagttcaat ctgatcccga ctctgtcggc cagagagaac gtagaggtgg cgcttgctcc
     9721 ccgcggcatc ccagccgcgc agcgccgcga gaccgcggtg cggctgctcg accaggtcgc
     9781 tctctccggc cgcgtggacc acctgccctc gcagctctcc ggcggcgagc agcagcgagt
     9841 ggcggtcgcc cgtgcgctct cgaacgagcc gcgcgtgctg ctcgcggacg agccgaccgg
     9901 caatctcgac agcgcgaccg gcgatgacct cttcgcgctg ctccgcagac tcaatgacga
     9961 gggccagacg atcgtgctga tcacgcatga ccgctcgatc gccgagcggg cgccgcgccg
    10021 gatcgagatg cgcgacgggc gcttgcagga cggtctgcaa ccgctcgccg gtgcatccgc
    10081 gggctagcct ggaagcgtga cgcgcgtact ggttgtcgat gatgagcgga gcgtgcgcga
    10141 tgcgatccgc cgcgcgctca cgctcgaggg ctacgaggtg gacctggccg acggcggcct
    10201 gtccgccctc agccacctga cagcgcggcc ggacgcgatg gtgctcgacg tgctcatgcc
    10261 ggacctcgac gggctcgagg tctgcaagcg cgtgcgcagc gcgggcgacc gcacgccgat
    10321 cctgatgctg accgcccgcg acggcgtgtc cgaccgcgtg gcgggcctgg acgccggcgc
    10381 cgacgactac ctcgtcaagc ccttcgcgct cgaggagctg ctcgcacgcc tgcgcgcgct
    10441 gctccggcgc agcctgccgg acggcgatgg cgacgtgctc gtgttctccg atctcatgct
    10501 cgacactgct gcccacgaag cccgccgcgg cgagcgccgt atagacctga cccgcaccga
    10561 gcaccagctg ctcgagctct tcatgcgtaa cccgcggcga gtgcttacgc gctcgcagat
    10621 caacgagcgc gtgtggggct acgacttcgg cccggcgtcc aattcgctcg gcgtctacgt
    10681 gggctacctg cgacgaaagc tcgaggaggg cggcgagccc cggctcattc ataccgtgcg
    10741 aggcgtgggc tacgcactcc gcgaggagga tcggtgacgc tgcgcaaccg cctcgctgtt
    10801 gctgccgcgg tggccgtggc gcttgcggtg gcgggcggct gcatcgcgtc gtacctggcg
    10861 gtgcgcgcga agatgcgcga cgaggtcgac acgtcgctgc gccaacgcgc gaacgagctg
    10921 tcgagcctcg cgcagaacgg tcccgggcag atggacgacg agcccttctc gaagcccgcg
    10981 cccgcgccgc gcttcggtgg tgcgccggga tacgcgcagg tggtcgcccc cgatggtgat
    11041 gtactgctcc caccgggcga gcggacgcaa ctcccggtgg ccggcgaggc tcgccgggtc
    11101 acgcagggga acaacgagcc ggtcctgcgc gacgtgcgcg tgaacggcgc acacctgcga
    11161 gtgataacgg ccccgctgcg agagggcttc gccatccaga tcgcccggcc gctcagcgag
    11221 gtggacagcg tcctgcactc gctcgtgatc gtgttcctgt tcatcaccgc ggcgggcgtc
    11281 gggctggccg tggtgctcgg ccgcctcgtg gcccgcaccg cgctcgcgcc ggttgagcgg
    11341 ttcacggcgc agactgagca gatcgccgag cacccggacg aggggcggcg gatcgacgtc
    11401 gagggcgagg atgagctcgc ccgcctggcc gcgagcttca acgcgacgct cgatgcgctc
    11461 gagcactcgc tcgcgtcgca gcgccagctc gtggccgacg cgtcgcacga gctgcgcacg
    11521 ccgatggcca gcgttcgcac gaacatcgaa gtgctcgcga aaggcgaccg gctcgacccc
    11581 acagagcgag agcgcctcgt gtcggacatc atcgagcaga tcgacgagct gaccgcgctc
    11641 gtggccgacg tcgtggagct ggcgcgcggc gcgaagccgg acgaggcgaa ggagcaggtg
    11701 cgcctcgatg agctggccgc tgcgtgcacc gaccgcgcgc gccggcacgc gcccgacgtg
    11761 acgttcgaga cgcgcctcga gccggtcgcc gtcaccggca atccgcagcg tctccatcgc
    11821 gcgatctcga acctgctcga caacgcggcg aagtggagtc cccccggggg cgtcgtggag
    11881 gtggaggtgc gcgacggctc ggtcaccgtg cgcgaccacg gccctggcat caagccacag
    11941 gaccttccac acgtgttcga ccgcttctac cgcgcccccg acgcgcgcag catgcccggc
    12001 tcagggctcg ggctagcgat cgtgaagcag gtggccgagt cgcacggcgg cacggccacc
    12061 gcgatgaacg ccgaagacgg cggcgcgctg ctgcggctca gcttcccgtc gtggacgcgg
    12121 ccgtcgacct ccgaaggacc ggcgctgggt gccagcgttc aacagccgca atcacaagtc
    12181 cgccacgaat gacttctgaa gacttttagg cgctatagcg accaaaagtc ttcgatcttt
    12241 cgtctggtcc gcccgttatc gctgccgttt acgcgacggc gagcgctacg ccgaggtcag
    12301 acgaccggga tgtcgacgat gaaggcctct tcgtcgacgt agcaccatga ccagtcctcg
    12361 ccgggctcgg ccgagcggac gatcgggtgt gagacctcgg ccgcgtgctt gctcgcgtgg
    12421 cggttcggcg agctgtcaca gcaaccgacg tgaccgcagg tgaggcaggt gcgcaggtgc
    12481 acccagcggt cgccggtctt gaggcactct tcgcagcccg caacctccgc aggaaccgct
    12541 tcgagcttga tcatgtcgag gtgcgtgcag ctcacgccgg ccatgaccta agactacgca
    12601 ccgcgtccag cggccaagcg gcgctcgatc tccggctgga gtgcggcggc catgtacgac
    12661 ccgtcgtaca cctcgccgtc gagaaagatc gtcggcgtgc cgcgcacgcc gctgtcgatc
    12721 ccggtccgca cgtcgtgctc gatgccgggc aggtactcgc tcgagtcgat gcccgcccgg
    12781 aacgccgcaa ggtccagccc gagcgttgag gcatacccct cgaggtcctc ccgctcgagc
    12841 gccttctggt gctcgaacag cagctcatgc atctcccaga acgagccgag cgcggcggca
    12901 gtctccgcgg cctgtgccgc cgcgagcgca tgcgggtgca cctcggtgag cgggaaatgc
    12961 cggaacacga agcggaaggc gccgtcgagc cggcgctcga gcacctccac cgagcggtac
    13021 gccgctcgcg tgtaggggca ctcgtagtcg ccgtacacga ggagggtggt ggcgccctcc
    13081 gccccgcggc tgtgatcgcg cgggccgacg gcctcggcca ggtcggtcac gccacctgta
    13141 cggctctcgc gccgaccccg gcccatttgc gcgcgggtag gctggtcacc actggccctg
    13201 attggagacg atgatgaaca tcggaatcat cggcagcgga cggatcggag cgacggttgc
    13261 gtggctgctc acccgggcgg gacatgaggt cgcactggcc aactcgcgcg gcccggacac
    13321 gctagcggag cacgtcggca agctcggccc gcgcgcgcac gccacgacgg ccgcaaaggc
    13381 ggcgaccttc ggcgacgtgg tggtcgtcgc cgtaccggtc aaggccttcc ccgacctccc
    13441 ggccgagcag ctgagcggga aggtcgtcgt ggatacgggc aactactacc cccagcgcga
    13501 tggcaacatc gaggagctcg acgacgacac cacgacctcc accgagctcc tcggccgcca
    13561 gctctcgggc gctcgcctgg taaaggcgtt caacacgatg tactgggaga acctgcgcga
    13621 cgacggtcgc ccggacgcgc cgcgcgacga tcggctcgcg gtgttcctcg caggcgacga
    13681 cgaagacgcg aaaaagatcg tgagcggcct gatcgaggag atcggattcg cgccggtcga
    13741 caccggctcg ctcgccgagg gcgggcgccg gcagcagccg ggctcgccca tctacggcag
    13801 cctgctgaag gccccagagg cggaagcggc gcttgcctag tccgcgacgc ggaaccagac
    13861 gatgatcatc ggctcgtcct cggtgagccg ccagccgggc tcgccgagca gaaccgcgat
    13921 ctcgtcgcgg catccgtcgt agtactcgcg ctggacggcg cgccaactct cgagcggctc
    13981 gctgcttccc gtctgggccg cgacgatgtc cttggtgatc tcgccgaacg gcacgccggc
    14041 catgcgcacg acctcgatcg tcgcccggcg ccggccggtg tggtccatca ccggaaggcg
    14101 ctgccccatg cgaggcgggc cgccctcgag atcccactcg cgcgcgagcg agatgaccgc
    14161 cgtcttctcg ccgctgatga ccttgtcgat caggcgttcg ccgagcccgc cgtcgtcctc
    14221 gtggccaaac tcgaggaacg gctcgcgcgc gtcgcgccag ggagaaagcg ggatggagac
    14281 ttcgagcctc gtgccctcac caggtgggct gtcgaccctg agcgtcccgc cgagcgcctc
    14341 gactcggctg gcgaggcccg acaaccccga
//
LOCUS       DASVXS010000069         6697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_786805, whole genome shotgun sequence.
ACCESSION   DASVXS010000069 DASVXS010000000
VERSION     DASVXS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786805"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VF752_03865"
     CDS             <1..496
                     /locus_tag="VF752_03865"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8854714.1"
                     /translation="IVGVTVHRYNARAHWRGSTGAGWEHYSRAHEVSAPPAEPTLTVT
                     TGEAQGDPRQLNPEQLLLMAASTCQLLWFLHLASKARIDVVEYRDEAEAEMHQDDKPV
                     RITHITLRPTVVVRVPEGGEPPSEERIRHLCDLAHRECYIANSLTSAVKVEPRLQLAH
                     AEAV"
     gene            complement(473..748)
                     /locus_tag="VF752_03870"
     CDS             complement(473..748)
                     /locus_tag="VF752_03870"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX8854715.1"
                     /translation="MSERVAKRVVAHGRVQGVFFRDSVRQHARQRGVAGWVTNRPDGA
                     VEAFFEGDPEAVESLVEFAREGPRSASVRHVEVEDAEPQGLDGFSVR"
     gene            complement(745..1989)
                     /locus_tag="VF752_03875"
     CDS             complement(745..1989)
                     /locus_tag="VF752_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX8854716.1"
                     /translation="MTTPAEAVRGERLWGDLMAMAEVGATPEGGSQRLALTDDDRAGR
                     ELFGEWARSAGLELRADRAGNLFARRPGSDPSRPAVMTGSHLDTQPNGGRFDGVFGVL
                     AGLEAVRALNDAGVETEAPIDVVVWANEEGGRFPEFCTGSAVYAGVESLDAVRALRAF
                     DGPAYGEELDRVGFAGDEEPGAAPAGAYVELHIEQGPRLENEGVPLGIVLGIRGIRKF
                     RVTLTGREQHAGSPMTGRRDALRAAARVIEALAALGDRSQGASITVGRIEVSPNAPSV
                     VAGLVELEVDVRGAGLDSPDGLVREAADAARDAVAASAVEAGIEPPYMEYGPVRFDPE
                     VLDVVRQAARRTGNPSLDMVSPAGHDAGYVAARVPTAMLFVPCRDGLSHHPAEWAEPE
                     HLALATRALADSLATLAGARAS"
     gene            complement(1986..2996)
                     /locus_tag="VF752_03880"
     CDS             complement(1986..2996)
                     /locus_tag="VF752_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX8854717.1"
                     /translation="MSASRETTEQAGIAPPQAHQAAMASAAEPPFLDELAEVWGAPWG
                     AADEVGPLRRVLVRRPGDELRAIREDAWDERLGALVDPEGRWYWTDRHAPDLDLVAQQ
                     HDGLVRALLAEGVEVDVADSLGGRFVKAVYARDPSITVPGGAIVGRMGVRMRRGEEPQ
                     VTRTLAGLGMPILGTITGTGTLEGGSFVKLRPGLAALGTSIRCNHEGERQLRALLELI
                     GVDLIRVPVAGYSIHIDLHFAMVDVDRALIDPAGLPYDFLVTLRELGIDTIEAARDEE
                     WGLNLLCLRPGRVLMAEGSPRTAELLDRAGVEVVTIPYDEIHKNGGGVHCSTIELVRE
                     PA"
     gene            complement(2993..4519)
                     /locus_tag="VF752_03885"
     CDS             complement(2993..4519)
                     /locus_tag="VF752_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8854718.1"
                     /translation="MAHVEPRRAVRPGVPDEDRRPTQEETPYLDALRAYAARKPGRFH
                     VPGHKGGPAADPALREAVGERALEIDIPALMEGIDAGPEPTPFSEAQRLAAEAWGARR
                     TWFLINGASQGNHVACLTLAHQGQRVAVQRNMHSSAIDGLVLSGLLPTFMAPEIDAEL
                     LIAHCVTPESLARALDETPGAVGAMVVSPTYFGAVADVEGLAEVAHECGVPLVVDEAW
                     GAHLAFSDRLPVNALRLGADLVISSTHKIVGSITQSAMLHLGPDSMLDERVVDRCVTL
                     IESTSPNSLLCGSLDAARRHAAVHGAELLDETLRALDVTREAVREIPGLDVLDERIAG
                     RPGAFAYDPLRLVVDVRGTGATGYELAPLMLGQDDINLELFGENVLVAVFGMGESGAE
                     QGVRLVEALRKALDSLDVPGDRRREDFAPPPAWGPFELSPRDAFLGPQEVVRLTDAVG
                     RVAAESLAAYPPGIPNVLPGERLTAETVDYIQQTLQLGGTLRGASDRSFETIRVVVEE
                     "
     gene            complement(4531..4968)
                     /locus_tag="VF752_03890"
     CDS             complement(4531..4968)
                     /locus_tag="VF752_03890"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX8854719.1"
                     /translation="MGVVGPGEASPDELHAAHDVGRRLAELGAQVVCGGLGGVMEAAC
                     RGAKEGGGRTLGLLPGNDRRAANRYVDVAVPTGLGEARNALVVRAADGLIAVGGAYGT
                     LSEIALALKAGKPVVALGSWEIDGVTAAESPADAVERLRSALA"
     gene            complement(4986..5588)
                     /locus_tag="VF752_03895"
     CDS             complement(4986..5588)
                     /locus_tag="VF752_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854720.1"
                     /translation="MRHRIEAGPLIGALGALILLVSLFLDWFEPDLSAWTVFEVLDLV
                     LAGLALAALIPVARSITPNVPPGPRHDLLIIGLAALVVVASQLIQHPPAGVGRAPELG
                     AWLGLGGAVLMALGGALSEAKISVALDVEPRERATPAPHDEAASEAAAVEPEVQDELY
                     PEPRGGGPIGSDDPEPWRAGPDDKTTALDDEPPPPPRSGG"
     gene            complement(5646..>6697)
                     /locus_tag="VF752_03900"
     CDS             complement(5646..>6697)
                     /locus_tag="VF752_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8854721.1"
                     /translation="AAEDWVNTTCPRCGGPAKRETDTMDTFVDSSWYFLRYCDAHNDQ
                     AAWDRDVVDEWMPVDQYIGGVEHAILHLMYARFFTKALADLDLLGFQEPFANLFTQGM
                     ITRDGAKMSKSRGNVVSPRDYVERYGADTARCYILFIGPPDQDADWSDAGVAGINRFL
                     SRLYRLAVEVAERTDASELEPIGEPTALLRKAHWAIQKVTTDLGERFAMHTAISAVME
                     LVNELSSSKDVLYETPEGRSQVRFAAATAASLIFPFAPHLGSEAYELLTGRRVWEDPW
                     PEYDPALLETATVEVIVQVNGKLRDRIQVAAGATEEEQTEVARASQKVAQHLDGRAIV
                     KTVVVPGKLVNFVVR"
BASE COUNT          954 a         2503 c         2316 g          924 t
ORIGIN      
        1 gatcgttggc gtgacggtcc accgctacaa cgctcgcgcg cactggcgcg gctcgacggg
       61 cgcgggctgg gagcactact cccgcgccca cgaagtgagc gcgccgcccg ctgagccgac
      121 gctgaccgtg acgaccggcg aggcgcaggg cgacccgcgc cagctgaacc cggagcaact
      181 gctgttgatg gcggcgtcga cctgccagct gctgtggttc ctgcacctcg cgtccaaggc
      241 gcgcatcgac gtggtcgagt accgagacga ggcggaggcc gagatgcacc aggacgacaa
      301 gccggtccgc atcacgcaca tcacgctgcg accgacggtc gtcgtgcgcg tgcccgaggg
      361 cggcgagcct ccgagcgagg agcgcatcag gcatctgtgc gacctcgcgc atcgcgagtg
      421 ctacatcgcc aacagcctca ccagcgcggt gaaggtggag ccgcggcttc agctagcgca
      481 cgctgaagcc gtctaggccc tgcggctcgg catcctccac ctcgacgtgc cgcaccgatg
      541 ccgagcgcgg gccctcgcgc gcgaactcga cgagcgactc aaccgcctcg ggatcgccct
      601 cgaagaacgc ctcgactgcg ccgtcgggcc ggttcgtgac ccagcccgcc acgccgcgct
      661 gccgtgcgtg ctggcgcacg ctgtcgcgga agaagacgcc ctgcacgcgc ccgtgggcga
      721 ctacccgctt cgccacccgc tcgctcacga tgcccgtgct ccggcgaggg tcgcaagcga
      781 gtcggccagc gctctggtgg cgagcgccag gtgctcgggc tcggcccact cggcgggatg
      841 gtgactgagc ccgtcacggc atgggacgaa gagcatcgcg gttgggacac gcgcggccac
      901 gtaaccggca tcgtgacccg caggactgac catgtcgagc gaagggttcc cggtgcgccg
      961 cgcggcctgg cggacgacgt cgagcacctc ggggtcgaag cgcacgggcc cgtattccat
     1021 gtacggcggc tcgatgccgg cctcgaccgc gctcgccgcg accgcatcac gcgccgcgtc
     1081 cgcggcctcg cgcaccagcc cgtccggcga gtcgagtccc gcgccccgta cgtcaacctc
     1141 gagctcgaca agtccggcga cgacggacgg cgcgttggga gagacctcga tgcgcccgac
     1201 ggtgatgctc gcgccttgcg agcggtcgcc cagcgcggcg agcgcctcga tcacgcgagc
     1261 cgccgcccgg agggcgtcac ggcgcccggt catcgggctg cctgcgtgct gctcgcgccc
     1321 ggtcagcgtg acgcggaact tgcggatgcc ccggatgccg aggacgatgc cgagcggcac
     1381 gccctcgttc tccagccgtg gcccttgctc gatgtgcagc tccacgtagg cccccgcagg
     1441 cgcggctccc ggctcctcgt caccggcgaa gccgacccgg tcgagctcct cgccgtaggc
     1501 cgggccgtcg aacgcacgca gggcccgcac ggcgtcgagc gattcgacgc cggcgtacac
     1561 cgccgagccg gtgcagaact ccggaaagcg cccgccctcc tcgttggccc agacgaccac
     1621 gtcgatcggc gcctcggttt ccacgccggc gtcgttgagc gctcgcaccg cctcgagccc
     1681 ggcgaggacc ccgaagacgc cgtcgaagcg gccgccgttc ggttgcgtgt cgaggtggct
     1741 gcccgtcatc accgccggcc gtgacgggtc gctgccggga cggcgggcaa acaggttccc
     1801 ggcgcgatcg gcgcgcagct cgaggccggc cgagcgcgcc cactcgccaa acagctccct
     1861 acccgcgcga tcgtcgtctg tgagcgcgag tcgctgtgag ccaccctccg gcgtggcgcc
     1921 cacctccgcc atcgccatga ggtcgcccca caggcgctcc ccgcgcaccg cctcggcggg
     1981 tgtggtcacg cgggctcgcg caccagctcg atggtcgagc agtgcacgcc gccgccgttc
     2041 ttgtggatct cgtcgtacgg gatggtcacg acctccaccc ccgcgcggtc gagcagctcg
     2101 gcggtgcgag gactcccctc ggccatgagc acgcggccgg ggcgcaggca gagcaggttg
     2161 agcccccact cctcgtcgcg cgccgcctcg atcgtgtcga tgccgagctc gcgcaaggtg
     2221 acgaggaagt cgtacggcag ccccgccgga tcgatgagcg ccctgtcgac gtcgaccatc
     2281 gcgaagtgga ggtcgatgtg gatgctgtag ccggcgaccg ggacgcggat cagatcgacg
     2341 ccgatcagct cgagcagcgc gcgcagctgc cgctcgccct cgtggttgca ccggatcgac
     2401 gtgccgagcg cggcgaggcc cggacgcagc ttcacgaacg agccgccctc gagagtgcct
     2461 gtgccggtga tcgttccgag tatcggcatg ccgagccccg cgagcgtgcg agtcacctgc
     2521 ggctcctcgc cgcggcgcat gcgtacgccc atgcggccga cgatcgcgcc gcctggcacg
     2581 gtgatcgagg ggtcgcgcgc gtagacggcc ttcacgaaac ggcccccgag cgagtccgcg
     2641 acgtccacct ccacgccctc ggccaggagc gccctgacca gtccgtcgtg ctgctgtgcc
     2701 acaaggtcca ggtccggcgc gtggcgatcg gtccagtacc agcggccctc cgggtccacg
     2761 agcgcaccga gacgctcgtc ccacgcgtcc tcgcggatcg ctcgcaactc gtcccctggc
     2821 cggcgcacga gcacccggcg cagcggcccg acctcgtcag cggcgcccca gggcgcgccc
     2881 cacacctcgg cgagctcgtc gaggaacggc ggctcggccg cactggccat cgccgcctga
     2941 tgcgcctgcg gcggcgctat gcccgcctgc tccgtcgtct cgcgggatgc gctcactcct
     3001 ccaccaccac ccttatcgtc tcgaagctgc ggtcgctcgc gccgcgcagc gtgccaccga
     3061 gctggagcgt ctgctggatg tagtccaccg tctccgcggt gaggcgctct ccagggagca
     3121 cgttcgggat gcccggcggg taggcggcga gcgactcggc ggccacgcga cccacagcat
     3181 ccgtcaaccg gacgacctcc tgcggaccga ggaaggcgtc cctgggcgag agctcgaacg
     3241 gcccccacgc cggcggcggc gcgaagtcct cgcggcggcg gtcacccggc acgtcgagcg
     3301 aatcaagcgc cttacgcagc gcctcgacca gccgcacgcc ctgctcggct ccactttctc
     3361 ccatcccgaa gaccgctacg agcacgttct caccgaacag ctcgaggttg atgtcatcct
     3421 ggccgagcat gagcggcgcc agctcgtagc cagtcgcgcc ggtgccgcgc acgtccacca
     3481 cgagccgcaa cgggtcgtat gcgaacgcgc cgggtcgccc ggcgatgcgc tcgtcgagca
     3541 catcgaggcc ggggatctcg cgcaccgcct cgcgcgtgac atccagagcc ctgagcgtct
     3601 cgtcgaggag ctccgccccg tgcaccgccg cgtgcctgcg agccgcgtcg agcgagccgc
     3661 agagcagcga gttcgggctc gtggactcga tcagcgtgac gcagcgatcc actacgcgct
     3721 cgtcgagcat gctgtcgggg ccgagatgca gcatggccga ctgggtgatc gagccgacga
     3781 tcttgtgcgt gctcgagatc acgaggtcgg cgccgagcct gagcgcgttc accgggaggc
     3841 gatcgctgaa ggcgaggtgc gcgccccagg cctcgtccac taccagcggc acgccgcact
     3901 cgtgcgccac ctcggcgagg ccctccacgt ctgcgaccgc gccgaagtaa gtgggcgaga
     3961 ccaccatcgc cccgaccgct ccgggcgtct cgtcgagcgc gcgggccagc gactcgggcg
     4021 tcacacagtg cgcgatcagc agctcggcgt cgatctcggg cgccatgaag gtggggagaa
     4081 gcccggagag gacgaggccg tcgatggcgc tcgagtgcat gttgcgctgc accgcgaccc
     4141 gctggccctg atgggcgagc gtcaggcagg ccacgtggtt gccctgcgac gcgccgttga
     4201 tcaggaacca ggtgcggcgc gcgccccagg cctcggccgc gagacgctgc gcctcggaga
     4261 acggcgtggg ctcgggtccg gcgtcgatcc cctccatgag cgcagggatg tcgatctcga
     4321 gggcgcgctc gcccaccgct tcgcgcaacg ccgggtcggc tgcgggcccg cccttgtggc
     4381 ccggcacgtg aaagcggccc ggcttgcgcg cggcgtacgc gcgcagtgcg tcgaggtagg
     4441 gcgtctcctc ctgcgtgggg cggcgatcct catccggcac gccgggacgg actgcccgcc
     4501 ttggttccac gtgggccata gggcgcgagt ctaggcgagc gccgaccgga gtctctcgac
     4561 ggcgtcggcg ggcgactccg ccgccgtgac gccgtctatc tcccaactgc cgagcgccac
     4621 gactggcttc ccagccttca gcgcgagggc gatttccgag agcgtgccgt aggcgccgcc
     4681 gaccgcgatg aggccgtcgg cggcccgcac cacgagtgcg ttgcgcgcct cgccgaggcc
     4741 cgtcggcacc gccacgtcca cgtaccggtt ggcggcgcgg cgatcgttgc cgggcaacag
     4801 tccgagcgtg cggccaccgc cctcctttgc gccgcgacag gcggcctcca tcacgccgcc
     4861 gaggccgccg cacaccacct gcgcacccag ctcggccagc cgccttccga cgtcgtgcgc
     4921 cgcgtgcagc tcgtcggggg aggcttctcc cgggccgacg acccccacat atagggagtc
     4981 gccgctcagc ctccgctccg tggaggcgga ggcggttcgt cgtcgagcgc ggtcgtcttg
     5041 tcgtccggcc cggcgcgcca cggctcagga tcgtcggagc cgatgggccc gccgccgcgc
     5101 ggctcgggat acagctcgtc ctgcacctcc ggctcgacgg ctgccgcctc gctcgccgct
     5161 tcgtcgtgcg gcgccggggt ggcgcgttcg cgcggctcga cgtcgagcgc gacggagatc
     5221 ttcgcctccg acagcgctcc gcccagcgcc atgagtacgg ccccgccgag ccccagccac
     5281 gccccgagct cgggcgcacg cccgacgccc gcgggcgggt gctggatcag ctgcgaggcg
     5341 acgaccacca acgccgcgag cccgatgatc agcaggtcgt ggcgcggccc gggcggaacg
     5401 ttcggtgtga tcgaacgcgc gaccgggatc agcgccgcga gcgcgagccc ggcgaggacg
     5461 agatcgagca cctcgaacac ggtccacgcg ctcaggtcgg gctcgaacca atcgaggaac
     5521 aggctcacca gcaggatcag cgccccaagc gcgccgatca gcggccccgc ctcgatcctg
     5581 tgccgcatcg caacctccct agtcgccgtc cccccgcaat atgacggaag cggcaggcgc
     5641 ggcgcctagc gaaccacgaa gttcacgagc ttgcccggca ccacgaccgt cttcacgatc
     5701 gcgcggccgt cgagatgctg ggccaccttc tgcgacgcgc gggccacctc cgtctgctcc
     5761 tcttccgtcg cgccggcggc cacctggatg cggtcgcgca gcttgccgtt cacctgcacg
     5821 atcacttcga ccgtcgctgt ctcgagcagc gcggggtcgt actccggcca tgggtcctcc
     5881 cacacgcgcc gcccggtcag cagctcgtac gcctcggagc cgagatgcgg ggcgaacggg
     5941 aagatcagcg acgccgccgt cgccgcggcg aagcgcacct gggagcggcc ctccggtgtc
     6001 tcgtacagca cgtccttcga gctgctcagc tcgttcacaa gctccatcac cgccgagatt
     6061 gcggtatgca tcgcgaagcg ctcgcccaga tcggtcgtga ccttctggat cgcccagtgc
     6121 gccttgcgca gcagagcggt cggctccccg atgggctcga gctcggaggc gtcggtgcgc
     6181 tcggcgacct cgacggccag tcggtagagc cgcgaaagga agcggttgat cccggcgacg
     6241 ccggcgtcgg accagtcggc gtcctggtcg ggcgggccga tgaagaggat gtagcagcgc
     6301 gccgtgtccg cgccgtagcg ctcgacgtag tcgcgcgggc tcacgacatt gccgcgcgac
     6361 ttggacatct tcgcgccgtc gcgcgtgatc atgccctgcg tgaagaggtt ggcgaacggc
     6421 tcctggaagc cgagcaggtc gaggtccgcg agcgccttcg tgaagaagcg cgcgtacatc
     6481 aggtgcagga tcgcgtgctc gacgccgccg atgtactggt cgacgggcat ccactcgtcg
     6541 accacgtcgc ggtcccacgc cgcctggtcg ttgtgggcgt cgcagtagcg caggaagtac
     6601 caggacgagt cgacgaaagt gtccatcgtg tcggtttcgc gcttggcggg accgccgcac
     6661 cgcgggcacg tggtgttcac ccagtcctcg gccgccg
//
LOCUS       DASVXS010000070        33594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1289938, whole genome shotgun sequence.
ACCESSION   DASVXS010000070 DASVXS010000000
VERSION     DASVXS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 33594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33594
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289938"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /gene="mptB"
                     /locus_tag="VF752_03905"
     CDS             <1..1397
                     /gene="mptB"
                     /locus_tag="VF752_03905"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEX8854722.1"
                     /translation="ARVAAPIRAAQQPALGNSARASAGRAGPLALGGLCVVYLALALL
                     WAAPGSNLVLATAGGSPGWLLGPLRFGGIERMANGSLGGPLFYAGLWLALALYVVVLL
                     RRADLRARTVLWTIAGLHLLFLLAPPLLSQDVFSYIAYARLGVEHSLNPYTHSPSDIP
                     MDAIYPFAGSKDASSVYGPVFTLLTYPLSPLGVPAAFWVLKVVTAAASFGVVLLVWRT
                     AELLGRDPVLPAMIVGLNPHVVVHVVGGAHNEALMMLVAMGGVLAWVAGRRTLGPAIT
                     AAGAGVKATSLLFAPYFALAQTGRPRPEARRAVLRAVVASALALALVALIGLVGFGSH
                     ALDAVNVLSNNQGRTSRWSFPYKTAQLLGAVLPGDRLDYRDAVRSIYAAVFAGVAIWL
                     MWRTWRGDDPIRMAGWATFAILVASAWLVPWYLLWLLPLAALARDRRLVIASIALSAW
                     TLVIAVPLGTFGYG"
     gene            1390..2361
                     /gene="thiL"
                     /locus_tag="VF752_03910"
     CDS             1390..2361
                     /gene="thiL"
                     /locus_tag="VF752_03910"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX8854723.1"
                     /translation="MGELELIAAIERALAERGGTVLRGPGDDAAVARLPGAVSVTSID
                     AVVEGVHFRRETHSPADVGHKALAVALSDLAAMGADGAHAYVALAVPSWLTEPDALGL
                     VEAMERLAETTDTTIAGGDVVSGPALFAGVTVTGWAESEQALVYRDGANPGDIVGVTG
                     ALGGSGAGLLVLDGLEAGIDEATREALVTRHRRPVPRLAEGRALAAAGATAMIDVSDG
                     IATDAQHLAERSGAAVEIDLARLPLAEGVTAVAAAAGHNAAELAASAGEDFELLFSVP
                     ADRRAAIEAAAPVTWIGEVRQGSGLRLLDAAGREAQLSGYEHLGEPH"
     gene            complement(2643..4424)
                     /locus_tag="VF752_03915"
     CDS             complement(2643..4424)
                     /locus_tag="VF752_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8854724.1"
                     /translation="MIEPRIYRAAFLPALLAIVIGMFSLENRPAPLPQGLAADVLFDG
                     NMAVTGTRALVTSFPNRRPGTTGDLSSARSVAASFSHSGFDTRISHFEDSGKSLANVV
                     GTRVGATRRQIVVIAARDAASVPDATGSAADTAALLEMARVLEGRATRKTLVLVSVDG
                     STLGSVGARHFLQSDADRGLVDGVIVLSNLAAERSRGPLLVTWGNDATRTGISLKRTL
                     SNALVQELDKVPGDEHAFGQLVREAFPLGIGDQGPLVSDGLDAVRVSGSGELPPPHSE
                     RGVNDIDPNRVGSLGRGVLRAVASLDTGGKLRDGPHAYVVVGRKVLPGWSLAMLGFTL
                     ILPALVASIDAFARARRRRQAVGRWWRWVLAGSVPFLLGLVLAELLGLFGAAPDVSSS
                     PVSPDVNPLSGGAWAQLAAVAAVIAAGWAFVRVPLIRRVGASGDPATVGAPCAAALLM
                     SGLAVVTWLINPFAALFLLVPLHVWMLATLGPEDRRGAAPFLLVGLGFLPIALVALYY
                     MVRLQLGPLDSLWYLYQLVAGHHIGFASALIGCLVLGVFGSDVSTLIARARRPLPQAV
                     PAHPPIRGPGGHAGPGSLGGTRSAIRR"
     gene            4483..5184
                     /locus_tag="VF752_03920"
     CDS             4483..5184
                     /locus_tag="VF752_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854725.1"
                     /translation="MSTHPARILLVDDEQSVQKLLSYPLRKEGYEVVPALDGEEALDR
                     ARGQSFDLIVLDVMLPKMDGFEVCRKLRADSTVPIIMLTAKAEEFDKVLGLELGADDY
                     ITKPFSMREFRSRVKAVLRRAERAREEQREEEPLEDGELRIDFAKRTVQVRDETVRLT
                     YVEFEILSILARNPGRVFSRTALLDRIWGDSAYRDPRTIDVHIRHLREKLERDAKEPE
                     LIFTVRGVGYHFRDR"
     gene            5188..6612
                     /locus_tag="VF752_03925"
     CDS             5188..6612
                     /locus_tag="VF752_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8854726.1"
                     /translation="MFRLRSLINKIALVIFAITALVVGLIFLIVVPQLTSSLENQRLQ
                     DLDRVATASSRAFESVISLEISSGDLNDLVRSVSDGAEAEVTLLDTNPKSANGKQSVF
                     PLSNSRASRNFAYNQPLALRALKANDIESEISQVQGQRTAQVAVPLHVGGDKRPDWVV
                     LYSRTLGDVRDTVSLIRDRVLIASVIALVVALAVGYAVASAMARRVKRLETAANKLAA
                     GERVEPLPVDSDDELGQLTRSFNEMQAQLAKVERARREFIATASHELRTPIFSLGGFV
                     ELLQDEDLDPETREEFLATMSEQVARLQKLAVDLLDLSRIDAGSLELNPEDVNLGELA
                     RTIVGEFTPAVTQHHSDLELELEGDEVQAWCDRERVAQIMRILLDNAVRHTPEGTRIS
                     VSAGRENGAAELRVSDAGPGIRDSDQVFERFYTADAARGSGLGLAIAQELAERMSGHV
                     TVDSAPGHTTFTLELPTAKNGGTA"
     gene            6609..7793
                     /locus_tag="VF752_03930"
     CDS             6609..7793
                     /locus_tag="VF752_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8854727.1"
                     /translation="MTRGRLAAATACAVALVALPAAGCGGGGGSAGQSGPTTVKNTDV
                     KVVANGSKGGGLDAQTLYKQLSPGVVTVISLFSGGGGPLGGGGNGDAGLGSGFVLDGD
                     GYVATNAHVVTTGDKAPLRRAGQVFVEFADGNRVGAKIVGTDPDSDVALLKMDPKGLT
                     LTPLKFDDSANLVVGQPVAAIGSPFGEEQSLSVGVISALDRSIASLTEFQIGNAIQTD
                     AAINHGNSGGPLLDANGRVIGINSQIKSSSGGGEGVGFAIPANTAKRSLTQLRQSGKV
                     RYGFLGVSSQPLYPQLAEHLSLKVKRGALVSKVQAAPARRAGLKGATGAETFQGQDID
                     TGGDVIVSLDGEAIRSPGDLGDFVALRNPGDKVKLGIIRDGRARTLTVTLGSRPSGSQ
                     GG"
     gene            7786..8748
                     /locus_tag="VF752_03935"
     CDS             7786..8748
                     /locus_tag="VF752_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8854728.1"
                     /translation="MAEPAALRELVRELALALREQVRPQLGSHAARAHGEYAEGGDVT
                     FALDANAEELVHEFLIGAGRGVAYFSEDKGLVLPEGDAETVLVVDPIDGTRPALAGLE
                     SCCVSVAAAPLRGDVTMGEVTHACVVEIPSGAVFLAERGAGLVEGPPVRLSANERVDR
                     MFWSYGFRGRPARPMVEVLGDMIDLSSVGGGTFELGSACFDMTRVATGQLDAYVEPGP
                     RLVEEVPGLRAEFERVGGGAVLNNTPYDLAAAALCLDEAGALVSDAAGRPLSDRPLLG
                     SGADFQMSVVVSANQALHAQILEHLDAGLDRLRFTNARVEETEI"
     gene            8748..9125
                     /locus_tag="VF752_03940"
     CDS             8748..9125
                     /locus_tag="VF752_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8854729.1"
                     /translation="MTVESGTSTVRLTVPAKPEYVVLTRLALSAVCRLTSLQPEDVAD
                     LKLAITEAATGLVHDGSDAQLDCGFELHDDRLVLEVGGPAQSTVSAEEQELGRAIIEA
                     TVDEYEYGIDSVRLVKHLSPREQ"
     gene            9161..10399
                     /locus_tag="VF752_03945"
     CDS             9161..10399
                     /locus_tag="VF752_03945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854730.1"
                     /translation="MLQPVGVGHKSLADYTHLVGRPLVAEIKELAEQLQGLKVLHLSA
                     TAFGGGVSEILYTLVPLMRDVGIEAEWEIMIGREEFYNVTKLMHNSLQGADQEIDADG
                     WRIYEQYNRMNADELSGGWDVIIVHDPQPAAVRQHVPEKARTWVWRCHIDLSTPNLHT
                     MDGLIPFIKDYDGAVFHLQQYVPAALDGSSPHVYICPPAIDPLSPKNMALSPEDAAFV
                     CDQFGIDVDRPLMCQVSRFDPWKDPLGVIDAYRSVTAELPEVQLALVGSMATDDPEGW
                     EFFNSTLEYAQGDPDIKILNNLNNVGAIEVNAFQSQADVVIQKSTREGFGLTVTEALW
                     KARPFIGGDVGGIPLQVQNGETGFLVKTPEECAERSLEILREPDLGKKLGRAGKEHVR
                     KHFLTPRLLRDWLRIFAESK"
     gene            10396..11898
                     /locus_tag="VF752_03950"
     CDS             10396..11898
                     /locus_tag="VF752_03950"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX8854731.1"
                     /translation="MTRQTPIVLVSNRGPATFHRDESGQLAAARGGGGLVTALTGLVN
                     HRDALWVASAMTDEDAEASRSRGGRSFPCELDGTSYNVRLVESDPEAYDRFYNVVANP
                     LLWFIQHYLWDLSNAPDIRKDEIDAYDEGYCVVNNDLAEAVLDEIDGHDAVVMLHDYH
                     LYAAPRTIRQARPDVFLHHFVHIPWTQPDAWRILPRDMREDIYEGILSNDIIGLHTRS
                     YKRNFLLCCRELFGLDVDEEAGIVNFEGREVWVRAYPLPISAESFHRTAARAEVHEFE
                     QELLRRRREHLILRVDRADLSKNVLRGFTAFDMFLTQHPEFRENVTFIAQLMPSRQDV
                     PEYVEYLEKIEALVAVVNHRHGTTDWMPIDLKLRENLDEAIAAYKHYDLLIVNAMFDG
                     MNLVAKEGPLVNEHAGVSLLSENTGAHEELGQFSLSVNPFDVQEQADAIYRALIMSAD
                     ERQWRADGLKKIVTARNPGDWVDEQLRDIEAKRNGERPPPLEHELSTERI"
     gene            12353..12754
                     /gene="rpsF"
                     /locus_tag="VF752_03955"
     CDS             12353..12754
                     /gene="rpsF"
                     /locus_tag="VF752_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX8854732.1"
                     /translation="MPLYDLMLLLDPNATDERREEIVSNTRSTIEADGQLVGLHNWGM
                     RRMAYEIDHRGEADYRLFQLEAAPELIDRLSHTLKITDGVLRFRVIRLKPGAPPPPEP
                     RAQPARLESREEEGTAQVAARSAADAPARSR"
     gene            complement(12767..15004)
                     /locus_tag="VF752_03960"
     CDS             complement(12767..15004)
                     /locus_tag="VF752_03960"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8854733.1"
                     /translation="MDARSALSFLAEASAVLAGSLEYERTLARVASLAVPDFADWCSV
                     DVVQPDGTLRGITSRHPDKRKEEFLMELRRRYREQKGRSEGVAHVIATGEPELVSYVP
                     ADADEGLLSGPEEEPLYRALAASSYMIVPMSARGRTIGALTFLSLSADRHYTESDLDF
                     AQHLARRFALAVDNSRLYDEAESARAQLDTIFTSIPVGLALYDLDLRCLRVNQALAEM
                     NGIPAEEHVGKTVEELLGPETGPEVAEQYRRVIASGEPLIDVEHSGETLARPGEVRTW
                     LASYMPVRGSDGQVMGITVVVTDVTERRHLLDAERAARRRAAFLAQAGEILDSSLDYE
                     ETLTNVTRIAVPAIADWCAIQLVDERGVIRQVAVAHSDPERERYAWELSERFAPDPDA
                     PTGAPHVLRTGETEWVPEIPDEMLDEVIPDPELRRIIRDLGLTSAITAPLQSRGRTLG
                     VISFVTAESGRIFGADDVRLAEDVARRAGVAVENARLYTERSHIAHTLQTRLLPGRLP
                     EIPVGELAVRYRAAGELQEVGGDFYDVFDRDGGWLFVIGDVVGKGPEAAAATAFARYT
                     LRASAMPSGAPSEALHALNAAMQTEASDLCTVCVASLTCAPDRCEIVVALGGHPPGLL
                     LRVDGTVEPIGEFGTIVGAAVEERYTDVRAEMLPGDTLVLYTDGVIEAGPLEEHLGEQ
                     GLIDVLAGLRGRSAEEIVSEVERTAVESARGRTRDDIALIAFRFQPTSVAAGSGSAGS
                     AQASA"
     gene            complement(15113..15583)
                     /locus_tag="VF752_03965"
     CDS             complement(15113..15583)
                     /locus_tag="VF752_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854734.1"
                     /translation="MEPQEAPDPDSRAWLAALHADDPAERRRSEARLQRLVIRVARRE
                     LKRRGDPDATEVAEEAVEQAGERLRESLGDYSGASRFSVWAARFPIAELARRGSARRP
                     DCEECLDRLDEHVELELVGADPDGALPLVARHLHSCSACAEARAALLDFALRSG"
     gene            15704..16330
                     /locus_tag="VF752_03970"
     CDS             15704..16330
                     /locus_tag="VF752_03970"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8854735.1"
                     /translation="MADSTARDRRPGRPREDRATSAAIAATLDLVAENGIGGMTTDAV
                     AARAGVSKATMYRRWPSKDALLVDAVGSLVSEIAVPDTGTLREDVRLLLRRAVRLYAD
                     SRPEKLIPELVSEMRRNPDLADAVRSGFLAQRRAALAEILERARARGELRDDIDRELA
                     LDLLGGVIYYRFLITGGPLDDALADSLTEALLHGIAVPSPRNTRKGSR"
     gene            16327..17190
                     /locus_tag="VF752_03975"
     CDS             16327..17190
                     /locus_tag="VF752_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX8854736.1"
                     /translation="MKIHAIQTGTVAVKQRQPRGEGGDRTRLLRTMLDSRWTEPLPIL
                     AWAIEHPEGLIVVDTGETARVNEPGYLPSWHPYFRTNLREWIEPEQEIGPQLRRLGLS
                     PDDARWVVLTHFHTDHAGGMHHFPRSDMLVTRADFEYSSGRRGMVRGFLPQHWPDWFA
                     PTFIEFTDQAIGPFERSLPLTDAGDVVLVPTPGHTPGHVSVAVRTDEAIVLLAGDASY
                     NQALMLEGAIDGVSPDGAAAHDTLRRLQRLAESEPTVYLPAHDHRSAARLAAMEVARA
                     RREDSEAATVR"
     gene            17335..17787
                     /gene="ssb"
                     /locus_tag="VF752_03980"
     CDS             17335..17787
                     /gene="ssb"
                     /locus_tag="VF752_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8854737.1"
                     /translation="MAATNINRVVLTGNLTRDPELRSTSGGTSVCSLRIACNTRRRNA
                     SGEWEDKPNYFDVTVWGAQGENCATYLQKGRPVAVDGRLEWREWQDQQGNKRQSVDII
                     ADSVQFLGSRDGGENGGRFTPQSDVPADTGDFSPAPAGPGNSDDDIPF"
     gene            17801..18079
                     /gene="rpsR"
                     /locus_tag="VF752_03985"
     CDS             17801..18079
                     /gene="rpsR"
                     /locus_tag="VF752_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX8854738.1"
                     /translation="MAKARGGSRQQTRRRDRKGGPGSGRRKPCAFCRDKVDVVDYKDF
                     GTLRRVMSEKGKIRSARITGACRRHQRQLAIAIKRARELGLLPYVAER"
     gene            18082..18531
                     /gene="rplI"
                     /locus_tag="VF752_03990"
     CDS             18082..18531
                     /gene="rplI"
                     /locus_tag="VF752_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX8854739.1"
                     /translation="MAQAILLQPVETLGDRGDVVDVSPGYLRNFLVPRKLAQPATPGA
                     IAEAQRRIEAAKRAEEEQTERAAENAALLTKTVLTIRQQAGDDGRLFGSVTSQDIVDA
                     IKNARGLKLDRRKVQLEHPIKTTGTHMVTVEVADGVTADVKTIVTGE"
     gene            18589..19992
                     /gene="dnaB"
                     /locus_tag="VF752_03995"
     CDS             18589..19992
                     /gene="dnaB"
                     /locus_tag="VF752_03995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX8854740.1"
                     /translation="MAVSVAPDQGAGKLPPQNVEAETSVLGAILLAEQALDGVLIDVR
                     LKPDDFYRPRHQLIFRAMIRLKEKADPEAVDAVTVSEELARAGELEEAGGTAYIHSLP
                     STVPAAANARHYARIVKQHALMRRLLEASRDIADEVYSFSGDAHELVERAEATFFRIG
                     HEDRTGELRAIEDVLHDELDKLEMVSREGVALTGTPSGFKDLDELTGGFQPGNLIVLA
                     ARPSMGKSALVVNIAENAAVDHDRPVALFSLEMSETELAQRFIASQAKLNGDDLRKGR
                     VKPDRWPKVVKATERLARAPLYVDDSSDIGILELRAKARRLHARKELGLLVVDYLQLM
                     RPEDSSASRVEQIGQISRGLKILARELKIPVIAVSQLSRAVETRPDKRPLLSDLRESG
                     QIEQDADLVMFIYRDEYYNRDSDRPGEADVIVAKHRNGPVGDVVLTFLSRYPKFANLY
                     RERPGEPAIAGIREEGL"
     gene            19989..20771
                     /locus_tag="VF752_04000"
     CDS             19989..20771
                     /locus_tag="VF752_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8854741.1"
                     /translation="MTQPAGEPMFDHEPEKNGYADVSHIGIEAADPGFCPEGICDGSG
                     WILGDDNAARPCACREPRINRAHSRRMGTGIPKRFRGISFERKPVVDMDPFVLRTVRE
                     FTDRIAERLEQGRGLWFYGDVGTGKTSLAMLVSRTALEAGRSVAIYSVPRLLADIKET
                     YERDSGDSYLSFFRRLCDVDLLHLDDLGAEKRTEWVLEQLYSIVNERWQEESSIVVTT
                     NIIDMDELREQVGTRTVSRLTEICGEPIPIMGRDLRRATPLR"
     gene            20796..22100
                     /locus_tag="VF752_04005"
     CDS             20796..22100
                     /locus_tag="VF752_04005"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX8854742.1"
                     /translation="MPGLVVVGAQWGDEGKGKVTDLIAEQADVVVRFQGGNNAGHTIV
                     RGNEIYKFHLVPSGILYPRKLCLIGNGVVVDPKVIIAELDELRQRGIDTSGLRISANA
                     HLIMPYHMLLDHEGETKLGKLQIGTTRRGIGPAYADKAARLGIRVQDLLDEKILKQKI
                     ATALEPKRQSLRPYAKDPSLDLHTMTEQYLAYGHRIEAHIADTQRIVWDALDEDGLVV
                     FEGAQGTMLDIDHGTYPFVTSSNPIAGAACVGAGVGPRDIDDVWGIAKAYVTRVGAGP
                     FPTELDDDTGARLREVGAEFGTTTGRARRTGWLDLVALRYAARLNGLTGLVVTKLDVL
                     TGIDPLYVATRYLGPEGARFDEFPYHQSIVHKVRGDYVELPGWSEDISGARSIEDLPQ
                     AARDYLEFIADHIGVPVVMVSVGPERDQMIWTEAARRLQKVA"
     gene            complement(22102..22749)
                     /locus_tag="VF752_04010"
     CDS             complement(22102..22749)
                     /locus_tag="VF752_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854743.1"
                     /translation="MALRCRRRPLYGRGTTGLAIVAGISSLAVIGNEVARVWRRGSAP
                     LPTETPHVLGAAEEAVAETVTVAVAGYQRASERENALLNLFSSFVITFLSARGIAYLL
                     RDRSSLGPFRDVFLGRRHIHHFVPGILLTFAAGSVAIATRGERLKPKLALAFGTGMGL
                     TLDESALLLELDDVYWRREGVVGVQITLAVTALLASLVLAVRLLRRGEAVVLPDA"
     gene            complement(22740..23450)
                     /locus_tag="VF752_04015"
     CDS             complement(22740..23450)
                     /locus_tag="VF752_04015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8854744.1"
                     /translation="MLQPSDRAPGLLVGEGVQVPDGVEIGGNVVIHAGTVLGEGVRIQ
                     DGAILGKPVALGPLSTASREVPPPAVVEAGATICAGAVVLAGARIGEGAVVGDQAHVR
                     ERSSVGAGSVVGRGSAVDNDVRIGSRVRIQTGCYITANSVVEDEVFVAPGVMTTNDNT
                     AGRHGDDYPIVGATLRRACRVGGRAVLLPGVEVGEEAFVAAGAVVTRHVPPRTLVMGV
                     PARPMGEVGDEELIERWR"
     gene            complement(23434..24438)
                     /locus_tag="VF752_04020"
     CDS             complement(23434..24438)
                     /locus_tag="VF752_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8854745.1"
                     /translation="MSRPVQVGVVGLGYWGPNLARNFDRLPGSDLRWICDASAEALAR
                     WSPEFPRARATTDLEEMLADPELEAVAVATPVPAHAEAAQRVLASRKHCFVEKPLAQS
                     VAEAEAVVAAAREADRMLMVGHLLEYHPGVQMLKEIADSGELGDIRYLYSNRLNLGKL
                     RDNENALWSLGAHDVSVLLYLVDEEPTECHAFGESYMQPGVEDVAFAYLRFPSGIAAH
                     LHLSWLDPHKERRVTVVGSKKMATFDDMEIERKLTVYDKGFDQNFSSYGEYIQRSGDI
                     WSPRVSNEEPLRVECSHFIERVRDGGEPRSGGESGLRVVRVLEALQRSLDRSSRAAAV
                     "
     gene            complement(24435..25682)
                     /locus_tag="VF752_04025"
     CDS             complement(24435..25682)
                     /locus_tag="VF752_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8854746.1"
                     /translation="MKIGIVGLGYVGLPLAVAFCEAGHEVIGLDIDSRRVSMLESGES
                     YVEDISSEQVRGMGHLFAATTRYEDLRPADAVVIAVPTPLTRNREPDLGPLISAGTSL
                     AGVVRAGQLVVLESTTYPGTTRERLVPLLEESGLAAGRDLHVAYSPERVDPGRTDYTM
                     KSTPKVVGGLTPACLERAVALYSEVCDHVIPVSGPDTAEMTKLLENIFRSVNIALVNE
                     LAIVADRMGLDIWEVVDAAATKPYGFMRFDPGPGMGGHCLPVDPFYLAWRAREFEVQT
                     EFVELAGEVNQAMPYFCVEKISHALNDHAKPVRGSRIAIIGVSYKPGVGDLRESPALK
                     IMRLLGRYGAQLVYHDDFVPDLPEQGLSNMPLGEALSGADAAVIVTAHPDLDVQAIVD
                     TAPLVVDFRGVTRGIEAKNLVRL"
     gene            complement(25679..26758)
                     /gene="wecB"
                     /locus_tag="VF752_04030"
     CDS             complement(25679..26758)
                     /gene="wecB"
                     /locus_tag="VF752_04030"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX8854747.1"
                     /translation="MRIATIVGNRPQFVKAAAVSRRLREGHEELLIHTGQHYDDELSR
                     IFFEELGVPSPDRELGVGPGTPIEQTSRLLASLEPELLGYQPDLVLVYGDTTSTLAGA
                     LGAAQEQIPLAHVEAGMRSFDRRMPEELNRVLTDHASDILLCSTETAVRNLERESVMG
                     EAHLVGDVMADVSLAFADVAEQRSNALEEQGVEAGEYLLVTAHRAGNVDDPQRLAMLV
                     EVLEALPLRTIFPVHPRTRSRLEGTGLIARLQNAPNVKLAPPLGYLDFITLERHARAI
                     LTDSGGVQKEAYVLGVPCVTLRDTTEWVETVGSGWNVLTDLDREAVLRALERPPPSGE
                     RPELYGGGHAGEHIFQVLSAYTRRR"
     gene            26867..27034
                     /locus_tag="VF752_04035"
     CDS             26867..27034
                     /locus_tag="VF752_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854748.1"
                     /translation="MDSVVRRKLIVGAIEAVGMAADVARHVAWYVSYQIDGGAARPQP
                     EAPKPTVPRPS"
     gene            27048..27785
                     /locus_tag="VF752_04040"
     CDS             27048..27785
                     /locus_tag="VF752_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8854749.1"
                     /translation="MSALDIAAALAVTAGALIQSATGFGFALVAAPLLFAATTPERAV
                     GLMIVLAAEVNLLTLFGERRSAEPLTAESRGLLLFSAPGAVAGVLVLRSLSATALQVA
                     VTVGVLASLALRRIHRHRHYGPGGVRLAAAGLAAGALTTSTTTNGPPLVLYLLGRAAR
                     PTQVRDTLVVCFLGLSVIGALALVATGTLHAAPSPLALAGLVPLVVAGHLAGRRLFAG
                     LSDERYETALTALLLATALAGLLLAIF"
     gene            complement(27782..28591)
                     /locus_tag="VF752_04045"
     CDS             complement(27782..28591)
                     /locus_tag="VF752_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX8854750.1"
                     /translation="MADQELASLDGRIAPAEETAIPVTDEGFLRGDGVFEVARVYLGA
                     PYALREHFDRMERSAANLRLGERVPREELERETRELLAARGDAFDGCLRYVLTRSGRR
                     LIITEPLKDPPHDLAVCFVTYAPTRILDGVKSLSYASNMLATRIACERGFDEAVLVTP
                     HGRVLEAPTSSIFWVGDDGRLCTTPLADHILASITRHRLMGLVEVEERPVTADELLGA
                     NEAFLASTTREVHPIARIEDSRLESPGPRTREAVDAFAAHLAEVLGRPVAS"
     gene            complement(28676..29134)
                     /locus_tag="VF752_04050"
     CDS             complement(28676..29134)
                     /locus_tag="VF752_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8854751.1"
                     /translation="MATYQTTTTVVVNPEYVLDVLTDPDRIAVWSPIAFELEEFDGDR
                     LRSGSRARVTGKLAGRRLSFDVDIIEAEGNRLALRATGPVDLDVEDDVQQCNDTSELR
                     ALVSVRSAGGLLGRVISGATEGLLGAGMLNTAVARIAREAEAGPAPALAA"
     gene            complement(29316..29522)
                     /locus_tag="VF752_04055"
     CDS             complement(29316..29522)
                     /locus_tag="VF752_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854752.1"
                     /translation="MASQNSTRDSWDGTTQEIAAIHLQTDSNAVTGDPRTESERYAEG
                     CEERMLAMERTTRLTAWRRICFNR"
     gene            complement(29680..29752)
                     /locus_tag="VF752_04060"
     tRNA            complement(29680..29752)
                     /locus_tag="VF752_04060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29717..29719),aa:Lys,seq:ctt)
     gene            complement(29821..30180)
                     /locus_tag="VF752_04065"
     CDS             complement(29821..30180)
                     /locus_tag="VF752_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8854753.1"
                     /translation="MNFQINDESIDGDTHVIELGGEIDLYTAPEFKERMVQVIEDGKK
                     QVIVDLSKATFIDSTTLGVLVGGVKRLRPAGGTLALVCSDQNIVKIFEITGLDRVFPI
                     HATREEALSAVKQGGPA"
     gene            complement(30247..30921)
                     /locus_tag="VF752_04070"
     CDS             complement(30247..30921)
                     /locus_tag="VF752_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854754.1"
                     /translation="MLFDLKGRRRHVVQAVYLLLAVLIGGGLVLFGIGGGSLNGGLLD
                     AFKGGGGSGGDSAVKKRIENEKKQLALNPQNEIVLKALVRDNYQLAAAKVDPNTSQFD
                     KDANGDLQQAALYWQKYLATNPKKIDPSLAQTMIQVYGQGGLNKPAPAAQAAEVVAEA
                     APNPNAYLQLAQYATLAGQTRKAELAGQKALALAPKSQRSSVKALLKQYKTGGAQPTS
                     TTGGGG"
     gene            complement(30941..31711)
                     /gene="tatC"
                     /locus_tag="VF752_04075"
     CDS             complement(30941..31711)
                     /gene="tatC"
                     /locus_tag="VF752_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8854755.1"
                     /translation="MARRLRPVSHEDRLTVVEHLDELRTRIIVSLLAFVTAFGLCMWQ
                     RHAILDIVNKPLHGREPITLGVTEPFMTTVTLAAYAAIVIALPVILYQLYAFVLPAFS
                     PSERRVALPLMLMVPFLFVGGVVFGYFVVLPPALKFLLNFNQHDFNVQIRARDYYSFV
                     SLTLVSCGILFQVPVGILALTRLGVLTPAQLRKNRRYALLVCAVLAALLPSVDPVSML
                     IEMVPLVVLYELSILLASAFGRPSSADVSERLAQPEGS"
     gene            complement(31730..32071)
                     /gene="tatA"
                     /locus_tag="VF752_04080"
     CDS             complement(31730..32071)
                     /gene="tatA"
                     /locus_tag="VF752_04080"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8854756.1"
                     /translation="MPNIGPLEIIIVLVIVLVIFGPKRLPELGRSMGRGMREFKDSIT
                     GNDKDDEEDRPAELSRAPVDQGTAPTQTQRVHADPPAAPTGGAGQPTPPAVPADDTDP
                     AQPVQTGEPRG"
     gene            complement(32134..32715)
                     /gene="dcd"
                     /locus_tag="VF752_04085"
     CDS             complement(32134..32715)
                     /gene="dcd"
                     /locus_tag="VF752_04085"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX8854757.1"
                     /translation="MVLSDRSIREEIDSGRITIDPYDPAMIQPSSVDVRVDSRFRVFH
                     NARYPYIDVRQPMDDLTELVEVQGDDPFILHPGEFVLGQTLERVTLPDDLVARLEGKS
                     SLGRLGLLIHSTAGFVDSGFSGNLTLELSNVANLPITIYKGMPIGQISFMRMDGPVEH
                     PYGSDETGSKYQGQGEPTPSRFYLNFERESQGT"
     gene            32848..33279
                     /locus_tag="VF752_04090"
     CDS             32848..33279
                     /locus_tag="VF752_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854758.1"
                     /translation="MSHSEYNESVKRIVLPSGKTIEVVSFSEVAQELTARPEAPRIDE
                     APAHGLHVCCDCGSHLVYPTEWEEAGRDAWHVWLHCPNCDWVGDGVFEQDLLDEFDEE
                     LDRGMEELAADLARLTRSNMSEDIDRFVAALEADAILPMDF"
     gene            complement(33276..>33594)
                     /locus_tag="VF752_04095"
     CDS             complement(33276..>33594)
                     /locus_tag="VF752_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854759.1"
                     /translation="SGPPERTGKHRGADLLDGTITLPLILARRRDDSLRELDLRNDVT
                     DAGHAEEVCERIAETGALADAREVALRYVAKAKRSLEGLDLSPRQRTALELVADGVVE
                     RYA"
BASE COUNT         5204 a        11738 c        11699 g         4953 t
ORIGIN      
        1 gggcaagggt ggcggcaccg atcagggcgg cgcaacagcc ggccctggga aatagcgcgc
       61 gggcgagcgc gggccgagcc ggccctctgg cgctcggcgg cctgtgcgtc gtctatctgg
      121 cgctcgccct gctgtgggcc gcgcccggct cgaacctcgt gctcgccacg gcgggcggct
      181 cgcccggctg gcttctcggc ccgctgcgct tcgggggcat cgagcgcatg gccaacggga
      241 gcctcggcgg gccgctgttc tacgccgggc tgtggctcgc gctcgcgctg tacgtcgttg
      301 tgctgctgcg gcgcgccgat ctccgcgcgc gcacggtgct gtggacgatc gccggcctgc
      361 atcttctctt cctgctcgcg ccgccgctgc tgtcgcagga cgtgttctcc tacatcgcct
      421 acgcgcgact cggcgtcgag cactcgctga atccctacac gcactcgccg agcgacatcc
      481 cgatggacgc gatctacccg ttcgcgggct cgaaggacgc gagctcggtg tacgggccgg
      541 tgttcacgct gctcacctat ccgctctcgc cgctgggcgt accggccgcg ttctgggtgc
      601 tgaaggtggt caccgccgca gcctcgttcg gcgtcgtgct tctcgtgtgg cgaacggccg
      661 agttgctcgg ccgcgacccg gtcctccccg cgatgatcgt ggggctgaac ccgcacgtgg
      721 tggtgcacgt ggtcggcggc gcgcacaacg aggcgctgat gatgctggtg gcgatgggcg
      781 gtgtgctcgc ctgggtggcg ggccgccgca cgctcggccc ggcgatcacc gctgcgggcg
      841 cgggagtgaa ggccacctcg ctgctgttcg cgccgtactt cgcgctggcg cagaccggcc
      901 ggcctcggcc ggaggcgcgc cgcgcggtgc tgcgcgcggt cgtggcctct gcgctggcgc
      961 tcgcgctggt cgccctgatc ggtctcgtcg gcttcggctc gcatgcgctc gacgccgtga
     1021 acgtgctctc gaacaaccag ggccggacct cgcgctggag cttcccgtac aagactgccc
     1081 aactgctcgg cgcggtgttg cccggcgacc ggctcgacta ccgcgacgcc gtgcgcagca
     1141 tctatgcggc cgtgttcgcc ggcgtggcga tctggctgat gtggcgcacc tggcgcggcg
     1201 acgacccgat ccgcatggcg ggctgggcca cgtttgccat tctcgtcgcc tcggcctggc
     1261 tcgtgccgtg gtacctgctg tggctcctgc cgctcgcggc gctcgcccgc gaccggcgcc
     1321 tcgtgatcgc atcgatcgcg ctctccgcgt ggaccctcgt gatcgccgtt ccgctcggta
     1381 ccttcggcta tgggtgagct cgagctgatc gcagcgatcg agcgcgccct cgccgagcgt
     1441 ggcggcacgg tgctgcgcgg gcccggcgac gacgctgccg tggcacgcct gcccggagcg
     1501 gtgtccgtca catcgatcga cgccgtggtg gaaggcgtgc acttcaggcg cgagacgcac
     1561 tcgcccgccg acgtggggca caaggcgctc gccgtagcgc tttccgacct cgccgcgatg
     1621 ggcgccgacg gcgcgcacgc ctacgtggcg ctcgccgtcc cgtcctggct cacggagccg
     1681 gacgccctgg gactggtcga ggcgatggag cggctggcgg agacgaccga cacgacgatc
     1741 gcgggcggcg acgtggtgag cgggccggcg ctcttcgcgg gcgtgacggt gacgggctgg
     1801 gctgagagcg agcaggcgct cgtgtaccgc gacggtgcca atcccggcga catcgtgggc
     1861 gtgaccggag cgcttggcgg ctcgggcgcc gggctgctcg tgctcgacgg gctcgaggct
     1921 gggatcgacg aagcgacccg cgaagcgctc gtgacccgcc accgccgccc ggtgccgcgg
     1981 ctcgcagagg gccgcgcgct cgcggccgcc ggcgccacgg cgatgatcga cgtgagcgac
     2041 ggcatcgcga ccgacgcgca gcacctcgcc gagcgaagcg gcgccgcggt cgagatcgac
     2101 ctggcacgcc tgccgctcgc agagggagtg accgcggtgg cagccgcggc gggacacaac
     2161 gcggccgagc tcgcggcttc cgccggcgag gacttcgaat tgctcttcag cgttccggcc
     2221 gaccgccgcg cggcaataga ggcggccgcg ccggtcacgt ggatcggcga ggtgcgccag
     2281 ggaagcgggc tacgtcttct ggacgccgcc gggcgggaag cgcagctcag cggctacgag
     2341 catctgggtg agcctcactg agtcgcgcgt gcgcgccgac cagcgcagga tcgcgtcagc
     2401 gacggcgtgc gggtcgacga cgtattcccc gcgctccagc ctttcggcga gatcggtctg
     2461 accggcgttc atgagtcttc cttgacctga gcgagcgttc acgcgtatgc gggcaggctc
     2521 aggagagccg cctggatcag ccctcccgga acggccctct ttcggtgcct ccatgcccct
     2581 tatcggcgat acgtcattca ccttttcgcc ggtgcgccga tcgctaggac gctcgtccac
     2641 ggtcacctcc ggattgcgga tcgggtgccg cccagcgagc cgggccccgc atggccgccc
     2701 ggcccgcgaa tcggcggatg ggccggcacg gcctgcggca gcgggcgccg cgcgcgtgcg
     2761 atcagcgtgg agacgtctga cccgaagact ccgagcacga ggcagccgat cagcgcgctc
     2821 gcgaagccga tgtggtggcc cgcgaccagc tgatacaggt accagagcga gtcgagcggg
     2881 ccgagctgga gcctcaccat gtagtagagc gccacgagtg ctatcggcag gaagcccagt
     2941 ccgaccagca ggaacggggc ggctcccctg cggtcttccg gcccgagcgt cgcgagcatc
     3001 cacacgtgca gcggcaccag caggaacagc gccgcgaacg ggttgatcag ccaggtgacg
     3061 accgccagcc cgctcatcag cagtgcggcc gcgcagggcg cgccgaccgt tgcggggtcc
     3121 ccggaggcgc cgacccgccg gatcagcggc acgcgtacga acgcccagcc cgcggcgatc
     3181 acagccgcca cggccgcgag ctgcgcccag gcgccgcctg agagcgggtt cacgtcgggc
     3241 gacacgggcg aggacgacac gtcaggtgcg gcgccgaaga ggccgagcag ctctgcgagc
     3301 accagcccga gcaggaacgg caccgagccg gcaagcaccc agcgccacca gcgcccgacc
     3361 gcctggcgcc ggcgcctcgc gcgcgcgaag gcgtcgatcg acgcgacgag cgcgggcagg
     3421 atcagcgtga agccgagcat cgcgagcgac cagccgggaa gcaccttccg gcccacgacc
     3481 acgtaggcgt gcgggccgtc gcgcagcttg cctcccgtgt cgagagaggc gaccgcccgg
     3541 agcacgccgc gccccagcga gccgacgcgg ttcgggtcga tgtcgttcac gccgcgctcc
     3601 gagtgcggag gcggcagctc accgctcccc gagacgcgca ccgcatcgag gccgtccgac
     3661 accagcggtc cctggtcgcc gatccccagc gggaacgcct cgcgcacgag ctggccgaac
     3721 gcgtgctcgt cgcccggcac cttgtcgagc tcctgcacga gagcgttcga gagcgtgcgc
     3781 ttcaacgaga tcccggtgcg cgtggcgtcg ttgccccagg tcacgagcag cgggccgcgc
     3841 gagcgttcgg cggcgaggtt cgagagcacg atcacgccgt ccacgaggcc gcggtccgcg
     3901 tcgctctgga ggaagtggcg ggcaccgacg ctgccgagag tcgagccgtc cacagacacg
     3961 agcacgagcg tcttgcgcgt tgcgcggccc tccagcacgc gcgccatctc gagcagcgcc
     4021 gcggtgtcgg ccgcgctgcc ggtggcgtcg ggtactgagg cagcgtcgcg cgcggcgatc
     4081 accacgatct gccggcgggt cgcgcccacg cgcgtgccga ccacgttcgc gaggctcttg
     4141 cctgagtcct cgaagtggct tatgcgtgta tcgaagccgg aatgggagaa gctcgcggcg
     4201 accgagcgcg cggacgacag gtcgcccgtc gtcccggggc gccggttcgg gaagctcgtc
     4261 acgagcgcgc gcgtgcccgt gacggccatg ttgccgtcga acagaacgtc ggccgccagc
     4321 ccctgcggca gcggagcggg cctgttctcg agcgagaaca tcccgatcac gatcgccagc
     4381 agggccggta ggaacgccgc gcggtagatg cgcggctcga tcacgcttca ggcctcgcga
     4441 tcccctcaac ctacaatctc cctgggtcga cacaaacctc acatgagcac ccatcccgca
     4501 cgcatcctcc tcgtagacga cgagcagtcg gttcaaaagc tgctctccta cccgctgcgc
     4561 aaggagggct acgaggtggt gccggcgctc gacggcgagg aagcgctcga ccgtgcccgc
     4621 ggccagagct tcgacctgat cgtgctggac gtgatgctgc cgaagatgga cggcttcgag
     4681 gtgtgccgca agctgcgcgc cgacagcacc gtgcccatca tcatgcttac cgcaaaggcg
     4741 gaggagttcg acaaggtgct gggcctggag ctaggggccg acgactacat caccaagccc
     4801 ttctcgatgc gggagttccg cagccgcgtg aaggccgtgc tgcggcgggc ggagcgcgcc
     4861 cgcgaagagc agagggagga agagccgctc gaggacggcg agctgcgcat cgacttcgcc
     4921 aagcgcactg tgcaggtgcg cgacgagacg gtgcgcctca cctatgtgga gttcgagatc
     4981 ctgtcgatcc tcgcccgcaa ccccggccgc gtcttctcgc ggaccgcgct gctcgaccgc
     5041 atctggggcg actccgcgta tcgcgatcca cgcacgatcg acgtccacat ccgccacctc
     5101 cgggagaagc tcgagcgcga cgccaaggag ccagagctga tcttcacggt gcgcggcgtg
     5161 ggctaccact tccgcgaccg ctgagccatg ttcaggctgc gctccctgat caacaagatc
     5221 gctctcgtga tcttcgcgat caccgcgctc gtggtcgggc tgatcttcct gatcgtggtg
     5281 ccgcagctca cctcgagcct cgagaaccag cgccttcagg acctcgaccg ggtggcgacc
     5341 gcctcctcgc gcgccttcga gtccgtgatc agcctcgaga tctcgtcggg cgacctgaac
     5401 gacctcgtgc gctcggtgtc cgacggcgcc gaggccgagg tgacgctcct cgacaccaac
     5461 ccgaagtccg ccaacggcaa gcagagcgtg ttccccctgt cgaactcgcg tgcctcgcgc
     5521 aacttcgcct acaaccagcc gctggcgcta cgggcgctga aggcgaacga catcgagagc
     5581 gagatctcgc aggtgcaggg acagcgcacc gcgcaggtgg cggtgcccct ccacgtcggc
     5641 ggcgacaagc ggccggactg ggtggtgctc tactcgcgca cgctcggcga cgtgcgcgac
     5701 accgtgagcc tgatccgcga ccgcgtgctg atcgcgtccg tgatcgcgct ggtggtggca
     5761 ctggccgtgg gctacgcggt cgcctcggcg atggcgcggc gcgtgaagcg gctcgagacg
     5821 gccgccaaca agctggccgc gggtgagcgg gtggagccgc tgcccgtgga ctcagacgac
     5881 gagctgggcc agctcacgcg cagcttcaac gagatgcagg cgcagctcgc gaaggtggag
     5941 cgcgcgcggc gcgagttcat cgccaccgcc tcgcacgagc tgcgcacgcc gatcttctcg
     6001 ctcggcggat tcgtcgagtt gctgcaggac gaggacctcg atcccgagac gcgcgaggag
     6061 ttcctcgcca ccatgtccga gcaggtggcg cggctccaga agctcgccgt cgacctgctc
     6121 gacctctcgc gcatcgatgc cggctcgctg gagctgaacc cggaggatgt gaacctcggc
     6181 gagctggcgc gcacgatcgt gggagagttc acacccgccg tgacccagca ccactcggat
     6241 ctcgagctcg agctagaggg cgacgaggtt caagcgtggt gcgaccggga acgggtcgcc
     6301 cagatcatgc gcatcctgct cgacaacgcc gtccgccaca cacccgaggg cacgcgcatc
     6361 agcgtgtcgg ccggccgcga gaacggggcg gccgagctgc gcgtgtccga cgccggaccc
     6421 ggcatccgcg actccgacca agtgttcgag cgcttctata cggccgatgc ggcccgcggg
     6481 tccgggctag gcctcgcgat cgcgcaggaa ctcgcggagc gcatgagcgg ccacgtgacg
     6541 gtcgactcgg cgccgggtca tacgaccttc acgctcgagt tgcccaccgc caagaacggc
     6601 ggcacggcgt gacgcgcggc cggctcgctg cggcgacggc ctgtgccgtc gcactcgttg
     6661 ccctgcccgc cgccggctgc ggcggcggtg ggggctccgc cggtcaatcc ggcccgacga
     6721 ccgtcaagaa caccgacgtg aaggtggtcg cgaatggaag caagggcggc ggcctcgacg
     6781 cacagaccct ctacaagcag ctgtcgccag gcgtcgtgac cgtcatctcg ctgttctcgg
     6841 gcggcggcgg cccgctcggg ggcggcggca acggagacgc cgggctcggc tcgggcttcg
     6901 tgctcgacgg cgacggctac gtggccacga acgcgcacgt ggtcacgacc ggcgacaagg
     6961 ctccgctgcg gcgcgccggc caggtgttcg tggagttcgc cgacggcaac cgcgtgggcg
     7021 cgaagatcgt cggaacggat ccagactcgg acgtggcgct gctcaagatg gatccaaagg
     7081 ggctcacact cacgccgctc aagttcgacg actcggccaa tctcgtggtc gggcagccgg
     7141 tagcggcgat cggcagcccg ttcggcgagg agcagtcgct ctccgtcggc gtcatctcgg
     7201 ccctcgaccg ctcgatcgca tcgctcacgg agttccagat cggcaacgcg atccagaccg
     7261 acgccgcgat caaccacgga aactccggcg gcccgctgct cgacgccaac ggacgcgtca
     7321 tcgggatcaa ctcccagatc aagtcatcga gcggcggcgg cgagggcgtg ggcttcgcga
     7381 tccccgccaa cacggcgaag cgctcgctca cccagctccg ccagagcggc aaggtgcgct
     7441 acggcttcct cggcgtcagc tcgcagccgc tctatccgca gctcgcggag cacctgagcc
     7501 tgaaggtgaa gcgcggcgcg ctcgtgtcga aggtgcaggc cgcaccggcg cgccgggccg
     7561 gattgaaggg tgcgaccggc gccgagacgt tccagggaca ggacatcgac accggcggcg
     7621 acgtgatcgt gtcgctcgac ggcgaggcga tccgcagccc gggtgacctc ggcgactttg
     7681 tcgccctgcg caaccccggc gacaaggtca agctcggcat catccgcgac ggccgtgcgc
     7741 gcacgctcac cgtgacgctc ggctcgcgcc cgagcggctc ccagggtggc tgagcccgcg
     7801 gcgctccgcg agctcgtgcg cgagctcgca ctggcgcttc gcgagcaggt gcggccgcag
     7861 ctcggctcgc acgcggcccg tgcccacggc gagtacgccg agggcggcga cgtgacgttc
     7921 gcgctcgacg cgaacgcgga ggagctcgtg cacgagttcc tgatcggggc cggacgcggc
     7981 gtggcgtact tctccgagga caaggggctg gtgctgccgg agggggatgc cgagaccgtg
     8041 ctcgtggtcg acccgatcga cggcacgcgg cccgcactcg ccgggctcga gtcgtgctgc
     8101 gtgtccgtag cggcggcgcc gctgcgcggt gacgtgacga tgggcgaggt gacccacgcg
     8161 tgcgtggtcg agatcccgag cggcgccgtg ttcctcgccg agcgcggcgc cggcctcgtg
     8221 gagggaccgc cggtgaggct gtcggcgaac gagcgcgtcg accgcatgtt ctggtcgtac
     8281 ggcttccggg ggcggccggc gcgaccgatg gtggaggttc tcggcgacat gatcgacctg
     8341 tcttcggtcg gcggcggcac gttcgagctc gggtcggcct gcttcgacat gactcgcgtc
     8401 gccaccggcc agctggacgc ctacgtcgag cccggcccgc gcctcgtgga ggaggtgccc
     8461 ggcctccgcg cggagttcga gcgcgtcggc ggcggggcgg ttctcaacaa caccccgtac
     8521 gatctcgcgg cggccgcgct ctgcctcgac gaggcgggcg cgctcgtgag cgacgcggcg
     8581 ggccgcccgc tgtcggaccg cccgctcctc ggatccggcg ccgacttcca gatgtcggtg
     8641 gtggtgtcgg cgaaccaggc cctgcacgcg cagatactgg aacatctcga cgccgggctg
     8701 gatcgccttc ggtttacaaa cgctcgagtg gaggaaacgg agatctgatg acagtcgaga
     8761 gcggcacgag cactgtgagg ctcacagtgc cggcaaagcc tgagtacgtc gtgctcactc
     8821 gacttgcgct ctccgcggtc tgccggctca cgtcgctgca gcccgaggac gtcgccgacc
     8881 tcaagcttgc gatcaccgaa gccgccaccg gactagttca cgacggctca gatgcgcagc
     8941 tcgactgcgg gttcgagctg cacgacgatc gcctcgtgct agaggtcgga ggaccggctc
     9001 agtccacggt ttcggccgag gagcaggagc tcgggcgggc gatcatcgag gctacggtgg
     9061 acgagtacga gtacggaatc gactccgtca ggctcgtcaa gcacttgagc ccgcgcgaac
     9121 agtaggctcc gcgagtcctt cgtccaggta ggagaaaagg ttgcttcagc ccgttggggt
     9181 aggtcacaag tcgctcgcgg attacaccca cctggtgggg cggccactcg tcgcggagat
     9241 caaggagctc gccgagcaac tccaagggct gaaggtgctg cacctgtcgg cgacggcgtt
     9301 cggcggcggc gtctccgaga tcctctacac gctcgtcccg ctcatgcgcg acgtcggcat
     9361 cgaggccgag tgggagatca tgatcgggcg cgaggagttc tacaacgtca ccaagttgat
     9421 gcacaactcg ctccaaggcg cggaccagga gatcgacgcc gacgggtggc gcatctacga
     9481 gcagtacaac cgcatgaacg cggacgagct ctccggcggc tgggacgtga tcatcgtgca
     9541 cgacccacag cccgcggccg tgcgccagca cgtgcccgag aaggcgcgca cgtgggtctg
     9601 gcgctgccac atcgacctct ccacccccaa cctgcacacg atggacgggc tcatcccgtt
     9661 catcaaggac tacgacgggg cggtctttca cctgcagcag tacgtgcccg cggctctcga
     9721 cggatcctcg ccgcacgtgt acatctgccc gcccgcgatc gacccgctgt cgccgaagaa
     9781 catggccctc tcgcccgagg acgcggcgtt cgtgtgcgac caattcggca tcgacgtcga
     9841 ccgcccgctg atgtgccagg tgtcgcgctt cgacccctgg aaggatccgt tgggcgtgat
     9901 cgatgcgtac aggtctgtga cggccgagct gccggaggtc cagctcgcgc tcgtgggctc
     9961 catggccacg gacgaccccg agggctggga gttcttcaac tcgacgctcg agtacgccca
    10021 gggtgacccc gacatcaaga tcctcaacaa tctgaacaac gtcggagcta tcgaggtgaa
    10081 cgcgtttcag tcgcaggccg acgtggtgat ccagaagtcc acacgcgagg gcttcggcct
    10141 gaccgtgacc gaggcgctct ggaaggcgcg cccgttcatc ggcggcgacg tgggcggcat
    10201 cccgcttcag gtgcagaacg gcgagaccgg cttcctcgtg aagacgcccg aggagtgcgc
    10261 cgagcgctcg ctcgagatcc tgcgcgagcc cgacctcggc aagaagctcg ggcgagcggg
    10321 caaggagcac gtgcgcaagc atttcctcac gccgcgtctg ctgcgtgact ggctgcgcat
    10381 attcgctgag tcgaaatgac ccgacagact ccgatcgttc tcgtctcgaa ccgcggcccg
    10441 gccacgttcc acagagacga gagcggtcaa ctcgcggccg cgcgcggcgg cggtggcctg
    10501 gtgaccgcgc tcaccggact agtgaaccac cgcgacgcgc tctgggtcgc gtcggcgatg
    10561 accgacgagg atgccgaggc gagccgctcg cgcggcgggc gcagcttccc ctgcgagctg
    10621 gacggcacct cctacaacgt gcggctcgtg gagtcggacc cggaggccta cgaccgcttc
    10681 tacaacgtcg tggccaaccc gctcctctgg ttcatccagc actacctctg ggacctctcg
    10741 aacgcgccag acatccgcaa ggacgagatc gacgcctacg acgagggcta ctgcgtcgtc
    10801 aacaacgacc tggccgaagc ggtgctcgac gagatcgacg ggcatgacgc cgtggtgatg
    10861 ctccacgact accacctcta cgcggcgccg aggacgatcc gccaggcccg cccggacgtc
    10921 ttcctccacc acttcgtgca catcccctgg acgcagccgg acgcctggcg catcctgccg
    10981 cgcgacatgc gggaggacat ctacgagggg atcctgtcga acgacatcat cggcctgcac
    11041 acgcgctcct acaagcgcaa cttcctgctc tgctgccggg agctgttcgg gctcgacgtg
    11101 gacgaggagg cgggaatcgt gaacttcgag ggccgcgagg tgtgggtgcg cgcctacccg
    11161 ctgccgatct ccgcggagag cttccaccgg accgcggcgc gcgccgaagt gcacgagttc
    11221 gagcaggagc tgctccgccg ccggcgggag cacttgatcc tgcgcgtcga ccgtgccgac
    11281 ctctcgaaga acgtgctgcg cggcttcacc gccttcgaca tgttcctcac ccagcatccg
    11341 gagttccgcg agaacgtcac cttcatcgcg cagctcatgc cctcgcggca ggacgtgccc
    11401 gagtacgtgg agtacctcga gaagatcgag gcgctcgtgg ccgtcgtgaa ccaccggcac
    11461 ggcaccaccg actggatgcc gatcgacctg aagctgcgcg agaacctcga cgaggcgatc
    11521 gccgcctaca agcactacga cctcctgatc gtgaacgcga tgttcgacgg catgaacctc
    11581 gtcgcgaagg agggcccgct ggtgaacgag cacgcgggcg tctcgctgct gtccgagaac
    11641 accggcgcgc acgaggagct cgggcagttc tcgctctcgg tgaacccctt cgacgtgcag
    11701 gagcaggccg acgcgatcta ccgcgcgctc atcatgtccg cggacgagcg gcagtggcgg
    11761 gcggacggcc tcaagaagat cgtgacggcg cgcaacccgg gcgactgggt ggacgagcag
    11821 cttcgcgaca tcgaggcgaa gcgaaacggc gagcgcccgc caccgctcga gcacgagctg
    11881 tccaccgagc gcatctaagg cccgaactcg ccgccgacgg cgggtgcggg ctgtggaggg
    11941 agcggagccg gcgacggtgc gggctggggc atggccggcg caggagcggg cgcgggcacg
    12001 accgcctccg cggcgcggtt acgccggccg tgccgcttcg gatggcgcgc gtgacggcgc
    12061 gaccgtgggc gcgactcacg cgcctccgcg cgccgcgaaa ccgcaggggg cgggtccgcg
    12121 accgccggct cgtcggccac cggtggctgc gccgtcgtgg gtccgcatcc cttcggcgga
    12181 cccacgagga tgagcaggcc ggcggcgagc acggcggcca gccgccctac attcgcccat
    12241 cgcacccggc caaggaggcc gggagccgct ccagctcgcc gccgtgccat gttccggtaa
    12301 gcgccggcgg agcggttctc gcccccgtgc cgctgctatg gtctcgcgcc gaatgccgct
    12361 ctatgacttg atgctgctgc tcgacccgaa cgccacggac gagcgtcgcg aggagatcgt
    12421 ctccaacacg cgctcgacca tcgaggcgga cgggcagctc gtcggccttc acaactgggg
    12481 catgcggcgc atggcctacg agatcgacca tcgcggcgag gcggactacc gcctgttcca
    12541 gctcgaggcc gcgcctgagc tgatcgacag gctctcgcac acgctgaaga tcaccgacgg
    12601 cgtgctccgc ttccgcgtga tccgcctgaa gcctggagcg cctccgccgc ccgagccgcg
    12661 cgcccagcct gcgcgcctcg agtcgcgcga ggaagagggc acggcacagg tggccgcccg
    12721 ctcggctgcc gacgctcccg cccgctcgcg ctaaccagcc ccgccgctag gcactcgcct
    12781 gcgccgagcc cgctgagccg ctgcccgcgg ccacgcttgt cggctggaag cggaacgcga
    12841 tcagcgcgat gtcgtcgcgc gtccgcccac gagcggactc gaccgccgtg cgctccacct
    12901 cactcacgat ctcctcggcg gagcggccgc gcaggcccgc cagcacgtcg atcaagccct
    12961 gctcgccgag gtgctcttcg agcggcccgg cctcgatgac cccgtcggtg tagagcacga
    13021 gcgtgtcacc cggcagcatc tccgcgcgca cgtcggtgta acgctcttcc acggccgctc
    13081 ccacgatcgt gccgaactcg ccgatgggtt cgaccgtgcc gtccacccgc agcagcagcc
    13141 cgggcgggtg gccgccgagc gccacgacga tctcgcagcg atcgggcgca caggtgaggc
    13201 tcgcgacgca gaccgtgcag aggtcggatg cttccgtctg catcgccgcg ttgagcgcgt
    13261 gcagcgcctc ggacggcgcg cccgagggca tcgcggatgc tcgcaacgtg tagcgcgcga
    13321 aggcggtggc cgcggccgcc tccgggccct tgccgaccac atccccgatc acgaacagcc
    13381 agccgccgtc gcggtcgaac acgtcgtaga agtcgccgcc cacctcctgc agctcgccgg
    13441 ccgcgcggta gcgcaccgcc agctcaccga ccgggatctc cggcaggcgg cctggaagca
    13501 ggcgcgtctg gagcgtgtgg gcgatgtggc tgcgctccgt gtagagccgc gcgttctcga
    13561 ccgccacacc ggcgcggcgg gcgacgtcct cggccagccg cacgtcgtcc gctccgaaga
    13621 tgcggcctga ctccgcggtc acgaagctga tcacgccgag cgtgcggccg cgcgactgga
    13681 gcggagcggt gatcgcggag gtgaggccca gatcgcggat gattcggcgc agctcgggat
    13741 caggaatcac ctcgtcgagc atctcgtccg ggatctccgg cacccattcg gtctccccgg
    13801 ttcggagcac atggggagcg ccggtgggcg cgtccggatc gggcgcgaag cgctcgctca
    13861 gctcccaggc gtagcgctcg cgctcggggt cggagtgcgc tacggccacc tgccggatga
    13921 ccccgcgctc gtccacgagc tggatcgcgc accagtcggc gatcgccggc accgcgatgc
    13981 gggtcacgtt cgtgagcgtc tcctcgtagt cgagcgagga gtcgaggatc tcgccggcct
    14041 gcgcgaggaa ggcggcacgc cgccgcgcag cgcgctcggc gtcgagcagg tggcgccgct
    14101 cggtcacgtc ggtcacgacc acggtgatgc ccatcacctg gccatcggac ccgcgcaccg
    14161 gcatgtagga cgccagccag gtgcgcacct cgcccggccg cgcaagagtc tcgcccgagt
    14221 gctccacgtc gatcagcggc tcgccagagg cgatcaccct tcggtactgc tccgcgacct
    14281 caggcccggt ttcgggtcca agcagctcct ccacggtctt gccgacgtgc tcctccgccg
    14341 gaatgccgtt catctcggcg agcgcctgat tcacccgcag gcagcggagg tcgaggtcgt
    14401 acagggcgag ccccaccggg atcgaggtga agatggtgtc aagctgcgcc cgggcgctct
    14461 cggcctcgtc gtagagccgc gagttgtcca cagccagggc gaaccggcgg gccaggtgct
    14521 gggcgaagtc gaggtcggac tcggtgtagt ggcgatcggc gctcagcgag aggaacgtga
    14581 gcgcgccgat cgtccggccg cgcgcgctca tcggcacgat catgtaggag ctggccgcga
    14641 gcgcacggta gagcggctcc tcctccggac ctgagagaag gccctcgtcg gcgtcggccg
    14701 ggacgtacga gacgagctcc ggctcgccgg tcgcgatcac gtgcgcgacg ccttcgctgc
    14761 ggcccttctg ctcgcggtag cggcgacgga gctccatcag gaactcctcc ttccgcttgt
    14821 ccggatggcg gctggtgatc ccgcgcaggg tgccgtcggg ctggacgacg tcaaccgagc
    14881 accagtccgc gaagtccggg acggcgagcg acgcgacccg cgcgagcgtg cgctcgtact
    14941 cgagcgaacc cgccagaacc gcgctcgcct cagccagaaa gctcagcgcg gatcgtgcat
    15001 ccatgacggc tcgttgctgc ggtgctggct ttgtcacgcc ccccgcgcgc cctcgctgaa
    15061 tacggcacgc ggcacggagg ctacatgcat tgcgcgcgcg gcggcggtga tttcagccgc
    15121 ttctgagcgc aaaatccagc aacgcggcgc gcgcctcggc gcaggcgctg caactgtgga
    15181 gatggcgcgc gacgagcggc agtgctccgt cgggatcggc tcccacgagc tcgagctcca
    15241 cgtgctcgtc gagccggtcg aggcattcct cgcagtcggg ccggcgcgcg ctaccgcggc
    15301 gtgcgagctc ggcgatcggg aagcgagcgg cccagacgct gaagcggctc gctcccgagt
    15361 agtccccgag gctctcgcgc agccgctcac ccgcttgctc gaccgcctcc tcggcgactt
    15421 cggtcgcgtc tggatcgccc cggcgtttga gctcgcgtcg cgccacgcgg atcaccagcc
    15481 gctggaggcg cgcctctgaa cgccggcgct cggccgggtc gtcggcgtgc aacgcagcga
    15541 gccacgcgcg cgaatcgggg tccggtgcct cttgtggttc cactgacgcc aaagcatgcc
    15601 gccggcggac gacgccatcg gccactgaca cgaactcctg accatgtcca cgacacgctg
    15661 cccgcctctt gacattacga aaccgtacgg taccgtttcg ttcatggccg actccactgc
    15721 acgcgaccga cggccgggac gcccgcggga ggaccgcgcg acgagcgccg cgattgcggc
    15781 gacgctcgac cttgttgccg agaacgggat cggcggtatg accaccgacg ccgtcgcagc
    15841 gcgcgcgggt gtgagcaagg ccacgatgta ccggcgctgg ccgtccaagg acgcgctgct
    15901 cgtggacgcg gtcggctcgc tggtaagcga gatcgccgtc ccggacaccg gcactctgcg
    15961 cgaagacgtc cggcttctgc tccggcgggc cgtgcggctc tacgcagact cgcggccgga
    16021 gaagctcatc cccgagctcg tctcggagat gaggcgcaac cccgacctcg ccgacgcggt
    16081 gcggtccggc ttcctggcac aacgccgggc cgcgctcgcc gagatcctcg agcgcgcgcg
    16141 ggcacgaggc gagcttcgcg acgacatcga ccgtgagctc gcgctcgacc tgctcggcgg
    16201 cgtcatctac taccgcttcc tgatcaccgg cggtccgctc gacgacgccc tcgcggacag
    16261 cctcaccgag gcactgctgc acgggatcgc cgtgccatct ccccgaaaca cccgaaaggg
    16321 atcacgatga agatccacgc catacagacc ggaaccgtcg cggtgaagca gcgccagccc
    16381 aggggcgaag gcggcgatcg cacgcgtctg ctcaggacca tgctcgactc gcgctggacc
    16441 gagccgctgc cgatcctcgc ttgggcgatc gagcatccgg aggggctcat cgtggtcgac
    16501 acgggcgaga cggcacgcgt gaacgaaccc ggctacctgc cgtcctggca tccctacttc
    16561 cggaccaacc tgagggagtg gatcgagccc gagcaggaga tcgggccgca gctgcgccgc
    16621 ttggggctgt cgcccgacga cgcccgctgg gtggttctca cgcacttcca cacggaccac
    16681 gccggcggaa tgcatcactt cccgcgcagc gacatgctcg tcacgcgcgc cgacttcgag
    16741 tattcgtcgg ggaggcgcgg gatggtccgc gggttcttgc cgcagcactg gccggactgg
    16801 ttcgcgccga cgttcatcga gttcaccgac caggccatcg ggcccttcga gcgcagcctg
    16861 ccgctcactg acgctggaga cgtcgtactc gtaccgacgc ccgggcacac gccgggccac
    16921 gtgtcggtcg ctgtccggac ggacgaagcg attgtgctgc tcgcaggcga cgcctcgtac
    16981 aaccaggccc tgatgctcga gggcgcgatc gacggcgtga gtcccgacgg agccgcggct
    17041 catgacactc tgcggcggct gcagcggctg gccgaatccg agccgacggt gtacctcccc
    17101 gctcacgacc accgctcggc cgcgcggctc gccgcgatgg aggtcgcgcg ggcccggcgc
    17161 gaggattccg aagcagcgac tgtgcgctaa agcgcacttg tgtgaacgga gagcgaaaac
    17221 tctccgacaa tccggctact tctctgtctc gaggcggctt tgcgtcggtg accccttgta
    17281 ccctcgcctc acgagcccaa gactgcccga tcagatccga aggagcacca gctaatggca
    17341 gcaacaaaca tcaaccgcgt cgtgctgacg ggcaatctca cccgtgatcc cgagctgcgc
    17401 agcacgagcg gcggcacctc agtctgcagc ctccgcattg cctgcaacac gcgccggcgc
    17461 aacgcctcgg gcgagtggga ggacaagccg aactacttcg acgtcaccgt gtggggtgcg
    17521 cagggcgaga actgcgcgac ttacctccag aagggccgtc ccgtcgcggt cgacgggcgc
    17581 ctcgaatggc gcgagtggca ggaccagcag gggaacaagc gtcagtcggt ggatatcatt
    17641 gccgattcgg tccagttcct cggatcgcgg gacggcggcg agaacggtgg tcgtttcacg
    17701 ccgcagagcg acgtgccggc cgacaccggc gacttctctc ctgcgcccgc gggccccggg
    17761 aactcggacg acgacatccc gttctaagga gttcattcaa gtggcaaagg cacgcggagg
    17821 cagccggcag cagacccggc gccgcgatag gaagggtggc cccggcagcg gccggcggaa
    17881 gccctgcgct ttctgccgcg acaaggtcga cgtcgtcgac tacaaggact tcggcaccct
    17941 gcgccgcgtc atgagcgaga agggcaagat ccgctcggct cgcatcacgg gcgcctgccg
    18001 caggcaccag cgccagctcg caatcgcgat caagcgcgcg cgtgagctgg ggctcctgcc
    18061 gtacgtcgcg gagcggtagg catggctcag gcgatcctgc tgcagccggt ggagacgctc
    18121 ggagaccgcg gcgacgtggt cgacgtctcc cccggctacc tgcgcaactt cctcgtccca
    18181 cgcaagctcg cccagcccgc cacgcccggc gccatcgccg aggctcagcg gcgcatagag
    18241 gcggcaaagc gcgcggagga ggagcagacg gagcgggccg cggagaacgc cgccctgctc
    18301 acgaagaccg tgctcacgat tcgccagcag gctggcgacg acgggcgtct gttcgggtcg
    18361 gtcacatccc aggacatcgt cgacgcgatc aagaacgcac gcggactgaa gctcgaccgc
    18421 cgcaaggtcc agctcgagca cccgatcaag acgacgggca cgcacatggt gaccgtagag
    18481 gtcgccgacg gcgtgaccgc agacgtcaag acgatagtta cgggcgaata ggtgctcgtg
    18541 gctcgcttga ccgcatctga ccccgaccgc cccgccgttt gaacgcgcgt ggcggtctcg
    18601 gtcgctcctg accagggcgc gggcaagcta cctccgcaga acgtcgaggc ggagacctct
    18661 gtcctcggcg ccatcctgct cgcagagcag gcgctcgacg gcgtcctgat cgacgtccgg
    18721 ctgaagccgg acgacttcta ccggccgcgc caccagctga tcttccgggc catgatccgc
    18781 ctgaaggaga aggcggatcc cgaggcggtg gacgccgtca cggtctccga ggagctggca
    18841 cgcgcgggcg agctcgagga agccggcggc accgcgtaca tccactcgtt gccgagcacg
    18901 gtcccggcgg cggcaaacgc gcggcactac gcgcgcatcg tcaagcagca cgcgctgatg
    18961 cgcaggctgc tcgaagcgtc gcgcgacatc gccgacgagg tgtactcgtt ctcgggcgac
    19021 gctcacgagc tcgtcgagcg ggccgaggcg acgttcttcc ggatcggcca cgaggaccgc
    19081 acaggcgagc tgcgcgcgat cgaggacgtc cttcacgacg agctcgacaa gctcgagatg
    19141 gtctcgcgcg agggcgtggc gctcaccggg acgccgtcag ggttcaagga cctcgacgag
    19201 ctcaccggcg ggttccagcc cggcaacctg atcgtgctcg ccgcgcgccc gtcgatgggc
    19261 aagtcggcgc tcgtggtgaa catcgccgag aacgccgcgg tggatcacga ccgcccggtc
    19321 gcgctgttct cgctcgagat gtcggagacc gagctggcgc agcgcttcat cgcttcgcag
    19381 gcgaagctga acggcgacga cctgcgcaag ggtcgcgtca agcccgaccg ctggccgaag
    19441 gtcgtgaagg cgaccgagcg tctcgcgcgc gcgcctctgt atgtggacga ctcgagcgac
    19501 atcggcatcc tcgaactgcg cgcgaaggcg cggcgcctgc acgcgcgcaa ggagctcggc
    19561 ctcctcgtcg tcgactacct gcagctgatg cgtcccgagg acagctcggc cagccgcgtc
    19621 gagcagatcg gccagatcag ccgtgggctg aagatcctcg cgcgcgagct gaagatcccg
    19681 gtgatcgccg tctcgcagct gtcccgtgcg gtggagactc gtccggacaa gcggccactg
    19741 ttgtcggacc tccgcgagtc cggccagatc gagcaggacg cggacctcgt gatgttcatc
    19801 taccgcgacg agtactacaa ccgcgactcc gatcgtccgg gcgaggccga cgtgatcgtc
    19861 gccaagcacc gcaacggccc ggtcggcgac gtcgtgctca cgttcctgtc gcgctatccg
    19921 aagttcgcga acctctaccg cgagcgtccc ggtgagcccg cgatcgcggg cattcgcgaa
    19981 gaggggctat gacgcagccg gcgggggagc cgatgttcga ccacgagccg gagaagaacg
    20041 gctacgccga cgtctcgcac atcggcatcg aggcggccga ccccggcttt tgccctgagg
    20101 ggatctgcga cggcagtggc tggatcctcg gcgacgacaa cgccgctcgg ccgtgcgcat
    20161 gccgcgagcc acgcatcaac cgcgcgcaca gccggcgcat gggcaccggc atcccgaagc
    20221 gcttccgagg catctcgttc gagcgcaagc ccgtcgtcga catggacccg ttcgtgctgc
    20281 ggaccgtgcg cgagttcacc gaccggatcg ccgagcgcct cgagcaggga cgcgggctgt
    20341 ggttctacgg cgacgtcggc accggcaaga cttcgctcgc gatgctcgtg tcgaggacgg
    20401 cgctcgaggc cggccgctca gtcgcgatct actcggtgcc gcgcctgctt gcggacatca
    20461 aggagaccta cgagcgcgac tccggcgact cgtacctgag cttcttccgg cgcctctgcg
    20521 atgtggacct gctgcacctc gacgacctcg gggccgagaa gcgcaccgag tgggtgctcg
    20581 agcagctcta ctcgatcgtg aacgagcgct ggcaggagga gtcctcgatt gtcgtcacca
    20641 cgaacatcat cgatatggat gagctccgcg agcaggtcgg cacgcgaacg gtctcgcgtc
    20701 tcaccgagat ctgcggtgag ccgattccga tcatgggccg cgatctgcgc cgggcaacgc
    20761 cgcttcgtta gtccgctccc tacactcgcg atgaaatgcc aggactagta gttgtcggag
    20821 cacagtgggg cgacgaaggg aagggcaaag taaccgacct catcgccgag caggcggacg
    20881 tcgtcgtgcg cttccaaggt gggaacaacg ctgggcacac catcgtacgc ggcaacgaga
    20941 tctacaagtt ccacctcgtc ccctccggca tcctgtatcc gcgcaagctc tgcctgatcg
    21001 gtaacggggt cgtcgtggac ccgaaggtga tcatcgcgga gctcgacgag ctgcggcagc
    21061 gcggcatcga cacgagcggc ctgcggatct ccgccaacgc gcacctgatc atgccgtacc
    21121 acatgctgct cgaccacgag ggcgagacga agctcgggaa gctgcagatc ggcaccaccc
    21181 ggcgcggcat cggccccgcg tacgccgaca aggcggcgcg cctcgggatc cgtgttcagg
    21241 acctgctcga cgagaagatc ctcaaacaga agatcgccac agcgctcgag ccgaagcgcc
    21301 agtcgctgcg tccctacgcg aaggacccgt cgctcgacct gcacacgatg accgagcagt
    21361 acctcgctta cggccaccgc atcgaggctc acatcgctga cacgcagcgc atcgtctggg
    21421 acgcgctcga cgaggacggg ctcgtggtgt tcgaaggcgc ccagggaacg atgctcgaca
    21481 tcgaccacgg cacctatccg ttcgtcacct cgtcgaaccc gatcgcaggc gccgcctgcg
    21541 tgggcgcggg cgtggggccg cgcgacatcg acgacgtgtg gggcatcgcc aaggcgtacg
    21601 tgacgcgcgt gggcgcgggg ccgttcccga cggagctcga cgacgacacc ggcgcgcgcc
    21661 ttcgcgaggt cggcgcggag ttcgggacca ccaccggtcg cgcgcgccgc acgggctggc
    21721 tcgacctcgt cgcgctccgc tacgcggcgc ggctgaacgg cctcaccggc ctcgtggtca
    21781 cgaagctcga cgtgctcacc gggatcgacc cgctctacgt ggccacgcgc tacctcggcc
    21841 cggagggcgc gcgcttcgat gagtttccgt accaccagtc gatcgtgcac aaggtgcgcg
    21901 gcgactacgt cgagctcccg ggctggagcg aggacatcag cggcgcgcgt tcgatcgagg
    21961 atctgccgca ggccgcgcgc gactacctcg agttcatcgc cgaccacatc ggtgtgccgg
    22021 tcgtgatggt cagcgtgggt ccggagcgcg accagatgat ctggaccgag gccgcgcgcc
    22081 ggctccagaa ggtcgcctga ctcaggcgtc cggcagcaca accgcctcgc cgcggcgcag
    22141 cagcctgacc gccagcacca gcgacgcgag cagcgcggtc accgccaggg tgatctgcac
    22201 gcccaccacg ccctcgcggc gccagtagac gtcgtcgagc tccaagagca gagcggactc
    22261 gtccagcgtc agtcccatgc cggtgccgaa cgcgagcgcg agcttgggct tcagccgctc
    22321 gccgcgggtg gcgatcgcca ccgagccggc cgcgaacgtg agcaggatcc ccggcacgaa
    22381 gtggtggatg tgccggcggc cgaggaagac gtcgcggaac ggacccaggc tgctgcgatc
    22441 gcgcagcagg taggcgatgc cgcgcgcgga tagaaaggta atgacgaacg aactgaacag
    22501 gttgagcagc gcgttctccc gctcggacgc gcgctggtag cccgccacgg cgacggtgac
    22561 tgtttcggcc acggcctcct cggcggcgcc gagcacgtgc ggggtctcgg ttggcagggg
    22621 cgccgatccc cggcgccaca cgcgggcgac ctcgttgccg atcaccgcga ggctggagat
    22681 cccggcgacg atcgccaggc cggtggtgcc gcgaccgtag agcggccgcc gccgacacct
    22741 cagcgccacc gctcgatcag ctcctcgtcc cccacttcgc ccattgggcg agcgggtacc
    22801 cccatcacca acgttctagg cggcacatgg cgcgtgacca ccgcgccggc ggccacgaag
    22861 gcctcctcgc caacctccac ccctggcagc aggacggcac ggccgcccac gcggcaggcg
    22921 cggcgcagtg tcgcgccgac gatcgggtag tcgtcgccgt ggcggccggc ggtgttgtcg
    22981 ttcgtggtca tcacgcccgg cgcgacgaac acctcgtcct cgaccacgct gttggcggtg
    23041 atgtagcagc cggtctgaat ccgcacgcgc gagccgatgc gcacgtcgtt gtcgaccgcc
    23101 gagccgcgcc cgacgaccga accggcgccg accgacgagc gctcgcgcac gtgcgcctgg
    23161 tcgccaacga ccgcgccctc gccgatccgc gcgccggcga gcaccaccgc accggcgcag
    23221 atcgtcgcgc cggcctcgac gacggccggg ggcgggacct cgcgagaagc agtcgagagc
    23281 ggcccgagcg cgaccggctt gccgaggatc gccccgtcct ggatgcgcac gccctcgcca
    23341 aggaccgtgc cggcgtgaat cactacgttg ccgccgatct cgacgccgtc cggcacctgc
    23401 acgccctcgc cgaccagcag gccgggcgcg cggtcagacg gctgcagcac gcgagctccg
    23461 gtcgagcgag cgctgcagcg cctcgagcac tcgcaccacc cgcaggccgc tttcgccgcc
    23521 ggagcgcggc tcgccgccgt cccggacacg ctcgatgaag tgactgcact cgacgcgcag
    23581 cggttcctcg ttcgacaccc gcgggctcca gatgtcgccc gagcgctgga tgtactcacc
    23641 gtacgacgag aagttctggt cgaagccctt gtcgtacacc gtcagcttgc gctcgatctc
    23701 catgtcgtcg aacgtggcca tcttcttcga gcccacgacc gtgacacggc gctccttgtg
    23761 cgggtcgagc caggagaggt gcaggtgcgc ggcgatcccc gacgggaagc gcaggtaggc
    23821 gaaggcgacg tcctccaccc ccggttgcat gtagctctcc ccgaaggcgt ggcactccgt
    23881 cggctcttcg tcgaccaggt agagcagcac ggacacgtcg tgtgcgccga gcgaccacag
    23941 cgcgttctcg ttgtcgcgaa gcttcccgag gttcagccgg ttcgagtaga gatagcggat
    24001 gtcgccgagc tcgccggagt cggcgatctc cttgagcatc tgcacgccag ggtggtactc
    24061 gagcaggtgg ccgaccatca gcatccggtc ggcctcacgc gcggcggcca cgaccgcctc
    24121 ggcctcggcg acagactggg ccagcggctt ttcgacgaag cagtgcttgc gggacgcgag
    24181 cacgcgctgc gccgcctccg cgtgcgcggg caccggagtg gccaccgcga ccgcctcgag
    24241 ctccgggtcg gccagcatct cctcgagatc ggtggtggcg cgggcgcgcg ggaattcggg
    24301 cgaccagcgc gccagcgcct ccgccgaggc atcacatatc cagcgcaggt cggagccggg
    24361 cagtcggtcg aagttgcgag cgaggttggg gccccagtag ccgaggccga ccacgcccac
    24421 ctggacgggg cggctcacag gcgcacgagg ttcttcgcct ctatcccgcg cgtcacgccg
    24481 cggaagtcca caacgagcgg cgccgtgtcc acgatcgcct gcacgtcgag gtcgggatgc
    24541 gcggtcacaa tcaccgcggc gtcggcccct gatagcgcct cgccgagcgg catgttcgac
    24601 agcccctgct ccggcaggtc cggcacgaag tcgtcgtggt agacgagctg ggcgccgtag
    24661 cggccgagca gcctcatgat cttcaacgcg ggcgactcgc gcaggtcgcc tacacccggc
    24721 ttgtaggaga cgccgatgat cgcgatgcgc gaaccgcgca ccggcttcgc gtggtcgttc
    24781 agcgcgtgcg agatcttctc gacgcagaag tacggcatgg cctggttgac ctcgcccgcg
    24841 agctccacga actcggtctg cacctcgaac tcgcgggcac gccaggcgag gtagaaggga
    24901 tcgaccggca ggcagtgccc gcccatgccc gggcccgggt cgaagcgcat gaagccgtac
    24961 ggcttcgtgg cagcggcgtc gacgacctcc catatgtcga gccccatgcg gtcggccacg
    25021 atcgcgagct cgttgacgag cgcgatgttc accgagcgga agatgttctc gagcagcttg
    25081 gtcatctcgg cggtgtccgg gccggagacg gggatcacgt ggtcgcagac ctcgctgtac
    25141 agggcgacag cgcgctcgag gcacgccggg gtgaggccgc cgacgacctt cggggtgctc
    25201 ttcatcgtgt agtcggtgcg gccggggtcg acgcgctccg gcgagtacgc cacgtgcagg
    25261 tcgcggcccg cggcgaggcc ggactcctcg agcagcggga cgaggcgctc gcgcgtggtg
    25321 cccggatacg tggtcgactc gagcacgacc agctgccccg cgcgcacaac cccggcgagc
    25381 gaggtgcccg ccgagatcag cgggccgagg tcgggctcgc ggttgcgcgt gagaggcgtg
    25441 gggacggcga tgaccaccgc gtccgccggc ctgaggtcct cgtaccgcgt ggtggccgcg
    25501 aacaggtggc ccatcccgcg cacctgctcg ctggagatgt cctccacgta gctctcgccg
    25561 gactcgagca tcgacacccg gcgggagtcg atgtcgaggc cgatcacctc gtgccctgcc
    25621 tcacagaagg ccacggcaag cgggaggccg acgtagccga ggccaacgat cccgatcttc
    25681 atcgcctacg agtgtaagcc gacagcactt ggaaaatgtg ttctccggcg tggcccccgc
    25741 cgtagagctc gggacgctcc ccactcggcg gcggccgctc cagcgctcgc aaaacggcct
    25801 cgcggtcgag gtcggtgagc acgttccagc ccgatcccac ggtctccacc cactcggtgg
    25861 tgtcccgcag cgtgacgcac ggcacgccga gcacgtaggc ctctttctga acgccgcccg
    25921 agtcggtgag tatcgcgcgc gcgtgccgct cgagggtgat gaagtcgagg tagccgagcg
    25981 gcggtgcgag cttcacgttg ggcgcgttct gcaggcgcgc gatcaggccc gtgccctcca
    26041 gccgcgagcg cgtccgcggg tgcacgggga agatggtgcg gagcggaagc gcctcgagca
    26101 cctcgaccag catggcgagg cgctgggggt cgtccacgtt gcccgctcgg tgcgcggtca
    26161 cgagcaggta ctcgccggcc tccacgccct gctcttcgag tgcgttggag cgctgctccg
    26221 cgacgtcggc gaacgcgagc gacacgtcgg ccatcacgtc gcccaccagg tgcgcctcac
    26281 ccatcaccga ctcccgctcc agattgcgga cggccgtctc cgtcgagcag agcaggatgt
    26341 cgctcgcgtg gtcagtgagc acgcgattga gctcctccgg catgcggcgg tcgaaggagc
    26401 gcatgcctgc ctcgacgtgc gcaagcggaa tctgctcctg cgcggcaccg agggcgccgg
    26461 cgagcgtcga ggtcgtgtcg ccatagacca gcaccagatc cggctggtag ccgagcagct
    26521 cgggttcgag cgaggcgagc agccgcgacg tctgctcgat cggcgtgccc ggcccgaccc
    26581 cgagctcgcg gtcgggcgac ggcacgccga gctcctcgaa gaaaatccgg gagagctcgt
    26641 cgtcatagtg ctgcccggtg tgaatcagca gctcctcatg cccctcgcgg aggcggcgcg
    26701 acacggctgc ggccttcacg aactgcgggc ggttcccgac gatggtggct atgcgcatgg
    26761 ccgcgcgggg atcctagtaa ctgcagtttc ggcgcctctg gtcgatcacc ttggctagtg
    26821 cggatcagca tcaagtttcc gcagttcgac tgaatgcggg cgtatcatgg actcagtggt
    26881 caggcgaaag ctgatcgtcg gtgcaatcga ggccgtcggc atggccgcgg atgtcgcgcg
    26941 gcacgtggcc tggtacgtga gctaccagat cgacggcggc gcggcgcgac cgcagccgga
    27001 ggcgccgaag cccaccgtcc cgcggccgtc ctagctgtaa tgggcgcgtg agcgccctcg
    27061 acatcgccgc cgccctcgcc gtgacggcgg gcgcgctgat ccaatcggcc accgggttcg
    27121 gcttcgcgct ggttgccgcg cccctactgt ttgccgcgac cacgccggag cgcgcggtcg
    27181 ggctcatgat cgtgctggcc gccgaggtga acctgctcac gctcttcggc gaacgccgct
    27241 cggctgagcc gctgactgcg gagtcgcgtg ggctgctgct cttctccgcg ccaggcgcgg
    27301 tggccggcgt gctcgtgctg cgctcgctct ccgcgaccgc actgcaggtg gccgtcacgg
    27361 tcggcgtgct tgcgagcctc gcgctgcgcc gcatccaccg ccaccggcac tacgggccgg
    27421 gcggggttcg actggccgcg gccgggctgg cggcaggcgc gctcaccacc tccaccacca
    27481 cgaacgggcc gccgctcgtg ctctacctgc taggccgcgc agctcgccct acacaggtgc
    27541 gcgacacgct cgtcgtctgc ttccttgggc tgtcggtgat cggcgcgctc gcgctggtcg
    27601 ccaccggcac gttgcacgca gcgccgagtc ccctcgccct ggccgggctc gtgccgctcg
    27661 tggtcgccgg gcatctggct gggcggcgac tgttcgcggg gctttccgac gagcgctacg
    27721 agacggcgct cacggcgctg ctgctggcca cggcgctggc cggactgctg ctcgcgatct
    27781 tctagctcgc cacgggccgg ccgagcacct cggcgaggtg tgcggcgaag gcgtcgacgg
    27841 cctcacgcgt gcgcgggccc ggcgactcga gccgcgagtc ctcgatgcgc gcgatcggat
    27901 ggacctcgcg cgtggtcgag gcgaggaacg cctcgttcgc tccgagcagc tcgtcggcgg
    27961 tcacgggccg ttcctcgacc tccacgagcc ccatcagccg gtgccgagtg atcgaagcga
    28021 ggatgtggtc cgcgaggggc gtggtgcaca gccgtccgtc gtctccgacc cagaagatcg
    28081 aggaggtggg cgcctcgagc acacggccgt gcggcgtcac caggacggct tcgtcgaacc
    28141 cgcgctcgca tgcgatccgc gtcgcgagca tgttcgatgc gtacgacagc gacttcacgc
    28201 cgtccaggat gcgcgtcggc gcgtaggtga cgaagcaaac cgcgagatcg tgcggcgggt
    28261 ccttgagcgg ctcggtgatg atgagccgcc ggccgctgcg cgtgagcacg tagcgcaggc
    28321 agccgtcgaa ggcatcgccg cgggcggcga ggagctccct ggtctcgcgc tcgagctcct
    28381 cccgcggcac acgctcgccg agccgcagat tggcggccga gcgttccatg cgatcgaagt
    28441 gctcgcgcag cgcgtacggc gcacccaagt agacgcgcgc gacctcgaat acgccgtcgc
    28501 cgcggaggaa gccctcgtcg gttaccggga tcgcggtctc ctccgccggc gcgatgcgtc
    28561 cgtctagaga ggcgagctcc tgatcagcca tggcgcggca ccttactcca cgcccgcgcc
    28621 gctcctctcg gggggcggga ggagcggcgc gcggggcggg ggctgaggga ggggatcagg
    28681 cagcgagggc cggggcgggg ccggcctcgg cctcgcgtgc gatgcgggcg acggcggtgt
    28741 tgagcatgcc ggcgcccagg agcccttcgg tcgcgccgga gatgacgcgg ccgaggagcc
    28801 cgccggcgct gcgcactgag acgagagcac gcagctccga cgtgtcgttg cactgttgca
    28861 cgtcgtcttc gacgtcgagg tccaccgggc cggtggcgcg gagggcgagg cggttgccct
    28921 cggcctcgat gatgtccacg tcgaaggaga gccgccggcc ggccagcttg ccggtcacgc
    28981 gcgcgcggct gccgctgcgg aggcggtctc cgtcgaactc ctcgagctcg aacgcgatcg
    29041 gcgaccacac ggcgatccgg tccggatcgg tgaggacgtc gagcacgtac tccgggttca
    29101 cgacgacggt ggtggtggtc tggtaggtgg ccattgcggg agtcgatgca gcgagtgcga
    29161 gtcggtgcaa gatctgcgcg catactctct ccgcggatca ccctggccaa tgcggtggcc
    29221 tccacacttg gtggtggtgg tagccacact ccgcacaagg tcgggcggag cggcgctgca
    29281 gggcggcgct gcagggggag actcaggtca ccgcgtcagc ggttgaagca gatccgacgc
    29341 caggccgtga ggcgcgtcgt tcgctccatg gcgagcatgc gctcctcgca cccttcggcg
    29401 tagcgctccg actcggttcg cggatcaccc gtgaccgcgt tcgagtcggt ttgcagatgg
    29461 atggcggcga tctcctgagt cgttccgtcc cagctgtccc tggtggagtt ctgcgaagcc
    29521 atcacagttc gtgtgcccag cgcacatagg cagaaacccc cgcataccgg gccggtttcg
    29581 caagttccta cccgctgtac aaggccttgc agatgcttgg acgcggtgtc ccaaaccgag
    29641 cacgaaaaag gcccccggcg cggcgggggc ctcagcagat gggccgtgca ggaatcgaac
    29701 ctgcaacctt gggattaaga gtcccctgct ctgccaattg agctaacggc ccgggaacag
    29761 cgtgcaattg tattcgcagc ctgatgctgc gaacaggccc gcggaagcgc gcgaaacgcc
    29821 ctaggcgggg ccgccctgct tgaccgcgga gagggcctct tcgcgtgtcg cgtggatcgg
    29881 gaagacccgg tcgaggccgg tgatctcgaa gatcttgacg atgttctggt cgctgcacac
    29941 gagcgcgagc gtgccaccgg ccggccgcag gcgcttcact ccacccacga gcacgccgag
    30001 cgtggtggag tcgatgaacg ttgccttcga gaggtccacg atgacctgct tcttgccgtc
    30061 ctcgatgacc tggaccatgc gctccttgaa ctcgggcgcc gtgtagaggt cgatctcgcc
    30121 gcccagctcg atgacgtggg tatcgccgtc gatggactcg tcgttgatct ggaagttcac
    30181 ctgactcctc gtctctcgat tggcactgcg atgtgcgccg cctcgccccc cgagccggcg
    30241 tttgcgctag ccacccccac ccgtggtgct cgtgggctga gcgccgccag tcttgtactg
    30301 cttcagcagg gccttaacgc tactacgctg gctcttcgga gcgagcgcca gcgccttctg
    30361 ccccgcgagc tcggccttgc gcgtctgccc ggcgagcgtc gcgtactggg cgagctgcag
    30421 atacgcgttg ggatttgggg cggcctcggc caccacctcg gccgcctgcg ccgcgggtgc
    30481 gggcttgttc agcccgccct gcccgtagac ctggatcatg gtctgggcga gcgacggatc
    30541 gatcttcttc gggttcgtgg ccaggtactt ctgccagtag agggcggcct gctggagatc
    30601 gccgttcgcg tccttgtcga actggctggt gttcgggtcc accttcgccg ccgcgagctg
    30661 gtagttgtcg cgcacgagcg ccttgagcac gatctcgttc tgcggattca gtgcgagctg
    30721 cttcttctcg ttctcgatcc tcttcttcac cgcggagtcg cccccggagc cgcctccgcc
    30781 cttgaaggcg tcgagcaggc cgccgttgag cgagccgccg ccgatgccga acagcacgag
    30841 acccccgccg atgaggacgg cgagcagcaa gtagaccgcc tgcacgacat ggcggcgtct
    30901 gcctttcaga tcgaagagca tgcggtgcgc gtctcccggc ctacgagccc tcaggttgag
    30961 cgagtcgctc cgatacgtcc gccgacgagg ggcggccgaa ggcgctcgcg agaaggatac
    31021 tcagctcgta gagcaccacc agcggcacca tctcgatgag catcgagacc gggtcgacgc
    31081 tcggcagcag ggccgcgaga accgcgcaga cgagcagcgc gtagcgccgg ttcttacgca
    31141 gctgggccgg cgtgagcacg cccaggcgcg tgagggcgag gatgccgacg ggcacctgga
    31201 acaggatccc gcacgacacg agcgtgagcg acacgaagct gtagtagtcc cgcgcgcgga
    31261 tctgcacgtt gaagtcgtgc tggttgaagt tgagcaggaa cttcagcgcg ggcggcagca
    31321 ccacgaagta gccgaagacc acgccgccga cgaagaggaa cggcaccatc agcatgagcg
    31381 ggagcgccac gcgccgctcg ctcggggaga acgcgggcag cacgaaggcg tacagctggt
    31441 agaggatcac cggcagcgcg atcacgatcg ccgcgtaggc cgccagcgtg accgtcgtca
    31501 tgaacggctc cgtgacacca agggtgatcg gctcgcggcc gtggagcggc ttgttgacga
    31561 tgtcgaggat cgcgtgccgc tgccacatgc agaggccgaa cgcggtcacg aacgcgagca
    31621 gcgagacgat gatgcgcgtg cgcagctcgt cgagatgctc gacgaccgtg aggcggtcct
    31681 cgtgcgagac gggtctcagt cgcctggcca cttcacgcgg ccgcggcccc tagccgcggg
    31741 gctcgccggt ctggaccggc tgcgcaggat ccgtgtcgtc cgccgggacg gccggagggg
    31801 taggctggcc ggcgccgccg gtcggcgcgg cgggcggatc ggcgtgcaca cgctgagtct
    31861 gcgtgggcgc cgtgccctgg tcgacaggcg cgcgcgacag ctcggccggg cggtcctcct
    31921 cgtcgtcctt gtcgttgcca gtgatcgagt ccttgaactc gcgcataccg cgccccatcg
    31981 agcggccgag ctccggcaac cgcttcggcc cgaagatcac gagcacgatg actaggacga
    32041 tgatgatctc aagcgggccg atgttcggca taaggctgat gctcctccag aaggggttcc
    32101 ccaaccccta agggtagcgg cgaaaggcgg gcttcaggtg ccctgtgact cgcgctcgaa
    32161 gttgaggtag aagcggctgg gagtgggctc gccctgcccc tggtacttgc tcccggtctc
    32221 gtccgagccg taagggtgct ccaccgggcc gtccatgcgc atgaacgaga tctgcccgat
    32281 cggcatgccc ttgtagatcg tgatgggcag gttcgccacg ttcgagagct cgagcgtgag
    32341 attgccgctg aagcctgagt cgacgaagcc cgcggtcgag tggatcagca ggccgaggcg
    32401 cccgagcgag ctcttgccct cgagacgcgc cacaaggtcg tccggaagcg tgacccgctc
    32461 aagcgtctgc ccgagcacga actcccccgg atgcaggatg aacggatcgt cgccctggac
    32521 ctccaccagc tcggtgaggt cgtccatcgg ctggcgcacg tcgatgtagg gatagcgcgc
    32581 gttgtggaag acgcggaagc ggctgtccac gcgcacgtcg acgctcgacg gctggatcat
    32641 cgcggggtcg taggggtcga tcgtgatgcg gcccgaatcg atctcctcgc ggatgctgcg
    32701 gtcgctcaaa accacgggcg ggtgagtgta ttggctggcc ccggcgtcag ggctttctgc
    32761 gcaacctcga accgggtctc aagttcctcc gtgagcgacc gataccgggc gtacaggcag
    32821 cttcccccga gccgcacgcg agcaagcatg agccactccg agtacaacga gagcgtcaag
    32881 cggatcgtcc tgccgagcgg caagacgatc gaggtggtct ccttcagcga ggtcgcccag
    32941 gagctgaccg cgcgccccga ggcgccgcgg atcgacgagg cccccgcgca cgggctccac
    33001 gtctgctgcg actgtggctc gcacctggtc taccccaccg agtgggagga ggccggccgc
    33061 gacgcctggc acgtgtggct gcactgcccc aactgcgact gggtcggcga cggcgtgttc
    33121 gagcaggatc tgctcgacga gttcgacgag gagctcgacc gcggcatgga ggagctcgcc
    33181 gcggacctcg cccgcctcac ccgctcgaac atgtcggagg acatcgaccg cttcgtggca
    33241 gccctcgaag cggacgccat cctccccatg gacttctagg cgtagcgctc gaccacgccg
    33301 tcggcgacga gctcgagcgc ggtgcgctgc cgcgggctga ggtcgaggcc ctcgagcgac
    33361 ctcttcgcct tggccacgta gcggagcgcg acctcgcggg cgtccgcgag cgcgccggtc
    33421 tcggcgatcc gctcgcacac ctcctcggcg tgcccggcgt cggtgacgtc gttccggagg
    33481 tcgagctcgc gcaggctgtc gtcgcgccgg cgggccagga tcagcggcag cgtgatggtg
    33541 ccgtcgagca ggtccgcgcc gcggtgcttg cccgtgcgct ccggcggccc ggag
//
LOCUS       DASVXS010000071         8869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1290934, whole genome shotgun sequence.
ACCESSION   DASVXS010000071 DASVXS010000000
VERSION     DASVXS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290934"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF752_04100"
     CDS             <1..229
                     /locus_tag="VF752_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854760.1"
                     /translation="EYTAVANRSARRELLAPIRYTTLPERRLIPTVKGLAATAREAPE
                     VLREPAAWSLLAGSLLGARGVERLRRFKPAS"
     gene            259..1395
                     /locus_tag="VF752_04105"
     CDS             259..1395
                     /locus_tag="VF752_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX8854761.1"
                     /translation="MGTILLYGDTVRYPAMRHEVPLVVIDPFLFVQSDGRAHVLTSNL
                     ERDRIREVLPDADVLVLDELGFFELLEGGMQREEAVRETAVRALERWGVRHAVVPADL
                     AVAVADRLRSEGIELDVDGQAIDARRRAKTPSELAGITRAQRAAEAGMAAGEGLIRAA
                     QPRDGRLVYDGHELTAEEVRAAVRAACAEAGAPAPPEIMVISALSGGGHDPGSGPLPA
                     DLPIEIDLWPRDEDSGCWADMTRTFVNGEISDEVARLRDLARDSLEAAREAARPGVTG
                     RELYDRAAQVIEDAGYPTQRTRKPGETLTTGFYFSLGHGVGLEVHEPPALGLSGTDPL
                     VPGDVIAIEPGIEGLDGIGGVRYEDLLLITDDGCETLTRYSYDL"
     gene            complement(1370..1822)
                     /locus_tag="VF752_04110"
     CDS             complement(1370..1822)
                     /locus_tag="VF752_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854762.1"
                     /translation="MAGELARLEPLVGEWSIEASIPDAPLGRVVFEWALGGSFLLQRT
                     DVPHPDAPDSLCLIRYDDGSGVYSQHYFDSRGVVRLYAMTFGDGVWTLRRDAPDFSPL
                     NFHQRFIGRFWDDGDTIDGHWETSPDGVDWELDFTLIYRRIRDRTNSA"
     gene            complement(1825..2511)
                     /locus_tag="VF752_04115"
     CDS             complement(1825..2511)
                     /locus_tag="VF752_04115"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX8854763.1"
                     /translation="MKSVLVLSPHADDEAMGCSGLLAKLGSEGARIHIVYLAVDGFHH
                     WGLDRETSYEERVKEIDRVVELFGDNCSYEIAYRNQDLIEKLDTLPRRDLVDLFETRI
                     NEHRPDLLLVPAGEDYDQDHIAVFGAAFAAARPIGEQFGKWLVPHMLTYEMTKIQWAR
                     DPLPLRPAYVDITDFMETKLESVRRYETQFRPPPHIRSLDSVKALATIRGAEIGVMYA
                     EAFGVMRTVL"
     gene            complement(2508..3506)
                     /locus_tag="VF752_04120"
     CDS             complement(2508..3506)
                     /locus_tag="VF752_04120"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8854764.1"
                     /translation="MQRVIVTGGLGFIGSHVADAFLARGDEVVIIDSMVAAVTDGYEY
                     EEHPRCTVIRRPVEDYLADGGEFAGYDKVVHAASHVGPAGILKYQGRLGVEIVTTTQA
                     VLDASLAADCQLVVFSSAEVYGRSGQLAESDSIVVPTNYNARLEYAIAKTLTEAAAIN
                     SRVHGVEAIVVRPFNVAGARQSSAGGFVMPTFVQQALAGEPLTVFAGGEQVRAFLAAS
                     DLARFMVENLDDAFASGDTIFNLGNPGNATTIWNLAERTKELLGSDSEIIHADAEAIH
                     GPLYVEAESFEKLPVLSAAAKIGWKPQASLDQLILETSAEYREHGDARVERTASLP"
     gene            3808..4734
                     /locus_tag="VF752_04125"
     CDS             3808..4734
                     /locus_tag="VF752_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX8854765.1"
                     /translation="MTTAAEPLPAELPLPGGVIGSVVRLSAFLSATCLAPPAYLHRIQ
                     GRLASVRALGLGVSREDYVRIPIQSFLLEHPGVGHVLIDTGFHPSVAVDPKKNLGRLG
                     TLAFSGIEMDAADAVPARLRAMGIDPHDVGVVVMTHLHSDHASAISEFGGATFVFSRA
                     EWEAAGEPGGTRRGYVKSQFDHAFDYRTIDFDSPEVDSFATFGRAVDLFGDGSVRLVY
                     TPGHTRGHVSVIARLADREALVAGDAIYTMRTLREGAVPWLTQDEHRFKRSLREIQLY
                     AKQTPGALIVPGHDLEAFRAAERELSEVPGGA"
     gene            complement(4709..5497)
                     /locus_tag="VF752_04130"
     CDS             complement(4709..5497)
                     /locus_tag="VF752_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8854766.1"
                     /translation="MKPQVYKDPRPAEYFDRFHERARRRRPDWIYDFCRLVLTPIMVL
                     VYRTRAIDTDKVPSHGAAIVAPNHFSFLDHFFVAVYLRRKVQFMAKSQLFARPMQFIY
                     THGGVFPVRRGQRDEKSFETARKVLDRGDIVLMYLEGGRSRSGRLGEAKPGVGRLALE
                     TGVPVVPTAIVGSAKARNWKRGQFPKVTVQYGDAIRFEPVENPSREQAQAAATIVFAE
                     VEKLYYGVQEGGRKRAVRASRAARRAGRKAMDAAEAATRRPVPR"
     gene            complement(5510..6985)
                     /gene="glpK"
                     /locus_tag="VF752_04135"
     CDS             complement(5510..6985)
                     /gene="glpK"
                     /locus_tag="VF752_04135"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX8854767.1"
                     /translation="MILAIDQGTTGTKCIVFDDQARVAGRAYSEFQQHFPRPGWVEHD
                     AHEIWEVTRRVAHEALDAAGISGRDLDAIGITNQRETVVAWEPITGEPLHRALVWQDR
                     RTAARCAELREQGHEELFRSRTGLVLDPYFSGTKIEWLIKEAGVDPSACFGTIDSWLI
                     FKLTGHQATDYTNASRTLLFDIHDLVWDEELCGLLGVDAESLPDARPSSGVFGHTSEF
                     GGHVPVAGVAGDQQAALYGQACLQVGLGKNTYGTGSFVLQNAGTDAPDPPDGLLTTVA
                     WGANGRVDYALEASIFVTGAAVQWLRDGLGIISEAAETEPLARSLRSNDGVYFVPALT
                     GLGSPHWDPYARGTIVGLTRGSGRAHLARAALESIAYQTVDAVRATERAAGKELEELR
                     ADGGAAVNGWLMEFQADVLGVPVLVPEVSETTALGAAFLAGVAVGAWTEDDVQAIWRE
                     RARYEPRMSADERESLLDGWHRALERSRGWDQSSNESPQGG"
     gene            complement(6982..7659)
                     /locus_tag="VF752_04140"
     CDS             complement(6982..7659)
                     /locus_tag="VF752_04140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854768.1"
                     /translation="MRLELAAVVAARNEADRIADTLAALRRTFPDALLLVADDGSTDE
                     TAAIARAAGAEVHSTGSDIGKGGAMTEAVERVLAAGDPPEVLLLCDGDLGRTAEKLIA
                     LVHAVQDDIADVAVAAFLRRVGGGFGIALGFARWAVRRRCGRELDQPLSGQRALRGEL
                     VEHLLPFARGFGMETGMNIDALRAGMRVVEVEVELEHRATGRTAAGFAHRGRQLRDIA
                     RAYAKRR"
     gene            7704..7916
                     /locus_tag="VF752_04145"
     CDS             7704..7916
                     /locus_tag="VF752_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEX8854769.1"
                     /translation="MPDVEAHITGTVWKIECQIGQEVEEGDTLVILESMKMEMPVEAE
                     DEGVVSEIRCEEGQSVSEGDTLVVLE"
     gene            7913..8716
                     /locus_tag="VF752_04150"
     CDS             7913..8716
                     /locus_tag="VF752_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8854770.1"
                     /translation="MSPTQELASGKLLLDEPAERVARLRISNPAKRGALDHEILDTLA
                     ETVAHLDARCLIVTGDGHVFSAGYDIGNFDDGNFEEAAEKLVAHPFTAAIEALDAYPY
                     PTLAAVNGHAIGGGLELAVTCDIRLAASGVRMGMPPAKIGLIYSHTGLKKFIDACGVA
                     NTNELFHVGRNVDAERGRQMGLVNQVLEPEELERASVEMAAEIARNAPLSLAGNKRVI
                     RTLRDYPTRLPAEVERQLVELRESCFRSEDFREGIRAFAEKRQADWKGR"
BASE COUNT         1363 a         3071 c         3078 g         1357 t
ORIGIN      
        1 cgagtacacc gcggtcgcga accggtcggc gcgccgcgaa ctgctggcgc ccatccgcta
       61 cacgacgctc cccgagcgga ggctcattcc gaccgtgaag ggtctcgccg ccaccgcgcg
      121 tgaggcgccc gaggtgctgc gcgaaccggc tgcctggtcg ctgctggcgg ggagcctgct
      181 cggcgcccgt ggcgtcgagc ggctgcggcg attcaagccg gcgagctaaa cgcggcagtc
      241 cgctaaggtc cgcgcctcgt gggcacgatc ctgctgtacg gcgacaccgt tcgctatccg
      301 gcgatgcggc acgaggtgcc gctcgtcgtc atcgacccgt tcctgttcgt gcagagcgac
      361 ggccgcgccc acgtgctcac aagcaacctc gagcgggacc gaatcaggga ggtcctgccc
      421 gacgccgatg tgctcgtact cgacgagctc ggattcttcg agctcctcga aggcggcatg
      481 cagcgcgagg aagccgtgcg ggagaccgcc gtgcgtgcgc tggagcgatg gggtgtgagg
      541 cacgccgtcg tgcctgcgga tctggcggtg gcggtggccg accggctccg cagcgagggc
      601 atcgagctcg atgtcgatgg tcaggcgatt gacgcccgcc gccgcgcgaa gactccgagc
      661 gaactggcag gcatcacgcg cgcgcagaga gcggccgagg ctgggatggc ggccggcgag
      721 ggcctcattc gagcggccca gccgcgcgac ggccgactgg tgtacgacgg ccacgagctg
      781 actgccgagg aggtgcgcgc tgccgtgcga gccgcctgcg cggaggccgg cgcacccgcg
      841 ccgccggaga tcatggtcat atcggcactt tcgggcggcg ggcacgaccc gggctcggga
      901 ccgctcccgg ccgacctgcc gatcgagatc gatctgtggc cgcgcgacga ggacagcggc
      961 tgctgggcgg acatgacgcg cactttcgtg aacggtgaga tcagcgacga agtcgcgaga
     1021 ctgcgcgacc tcgcgcgcga ctcgctcgag gcggcccgcg aagctgcccg gcctggcgtc
     1081 accgggcgag agctctacga cagggcggcc caggtgatcg aggacgcggg gtatccgacc
     1141 cagcgcacgc ggaagcctgg cgagacgctc acgaccggct tctacttcag cctcggccac
     1201 ggcgtcgggc tcgaggtgca cgagccgccg gcgctcgggc tgtcggggac agacccgctc
     1261 gtgccgggcg acgtgatcgc catcgagccg ggcatcgaag ggctcgacgg gatcggcggc
     1321 gtccgctacg aggacctgct gctgatcacg gacgacggct gcgagacgct cacgcgctat
     1381 tcgtacgatc tctgattcgc ctgtagatca gcgtgaagtc gagctcccag tcgactccgt
     1441 cgggcgacgt ctcccagtgc ccgtcgatcg tgtcgccgtc gtcccagaag cggccgatga
     1501 accgctggtg gaagttgagc ggcgagaagt ccggtgcgtc cctgcggagc gtccagacgc
     1561 cgtcgccgaa cgtcatcgca tagagccgga ccacgcctcg tgaatcgaag tagtgctggg
     1621 agtagacgcc gctgccgtcg tcgtagcgga tcaggcagag gctgtccggg gcgtctgggt
     1681 gcggcacgtc ggtgcgctgg aggaggaagc tgccgcccag cgcccactcg aacaccaccc
     1741 gcccgagcgg agcgtccggg atcgacgcct cgatgctcca ctcgccgacc agcggctcga
     1801 ggcgagcgag ctcacctgcc atcgctacag aaccgtgcgc atcacgccaa acgcctcggc
     1861 gtacattacg ccgatctccg cgccccggat cgtggcgagc gccttcacgg agtcgaggct
     1921 gcggatgtgc ggcggcgggc ggaactgcgt ctcgtagcga cggacgctct cgagcttcgt
     1981 ctccatgaag tcggtgatgt ccacgtacgc aggccgcaac ggcagcggat cgcgcgccca
     2041 ctggatcttg gtcatctcgt aagtgagcat gtgcggcacg agccacttgc cgaactgctc
     2101 gccgatcggc cgggcagcgg cgaatgccgc gccgaacacc gcgatgtggt cctggtcgta
     2161 gtcctcgccg gccggcacga gcagcaggtc cggccggtgc tcgttgatac gcgtctcgaa
     2221 gagatcgacg aggtcgcggc gcggcagcgt gtcgagcttc tcgatcaggt cctggttgcg
     2281 gtacgcgatc tcgtacgagc agttgtcgcc gaagagctcg accacccggt cgatctcctt
     2341 cacgcgctcc tcgtagctcg tctcgcggtc gagtccccag tggtggaacc cgtcgaccgc
     2401 gagatacacg atgtggatgc gggcgccttc agacccgagc tttgcgagca ggcccgaaca
     2461 ccccatcgcc tcgtcgtcag cgtggggtga aaggaccagg acggacttca agggagcgag
     2521 gccgtgcgct ctacgcgcgc gtcgccgtgc tcgcggtact cggccgacgt ctcgagaatc
     2581 agctggtcga gcgacgcctg cggcttccag ccgatcttcg cggccgcgga gagcaccggc
     2641 agcttctcga acgactcagc ctccacgtac agcgggccgt gaatcgcctc ggcatccgcg
     2701 tggatgatct cggagtcgct gcccagcagc tccttcgtcc gctcggcgag gttccagatc
     2761 gtcgtcgcgt tgcctgggtt gccgaggttg aagatcgtgt cgccactggc gaaggcgtcg
     2821 tcgaggttct caaccatgaa gcgggcgaga tccgacgccg cgaggaaggc gcggacctgc
     2881 tcaccgccgg caaagaccgt gaggggctcg ccggccaggg cctgctgtac gaacgtgggc
     2941 atcacgaagc cgcccgcgct cgactggcgc gcgccggcga cgttgaacgg ccgcacgacg
     3001 atcgcctcga cgccgtgaac gcgcgagttg atcgccgcag cctcggtgag ggtcttggcg
     3061 atggcgtatt cgaggcgagc gttgtagttc gtcggaacca cgatcgagtc gctctcggcg
     3121 agttggccgc tgcggccgta gacctcggcg gagctgaaga ccacgagctg gcagtccgcc
     3181 gcgaggctcg cgtccaggac cgcctgcgtg gtggtgacga tctcgacgcc gagccgcccc
     3241 tggtacttca agatgcccgc cggtcccacg tgggaagcgg catgcacgac cttgtcgtag
     3301 ccggcaaact cgccgccgtc ggcgagatag tcctcgacgg gcctgcggat gactgtgcac
     3361 cgcggatgct cttcgtactc gtagccgtcc gtgacggccg ccaccatcga atcgatgatc
     3421 acgacctcat caccgcgggc gaggaatgca tccgcgacat gggagccgat gaagcccaag
     3481 ccccctgtca cgatcactcg ttgcatttgc gtccccctac ttccttatat ggcgatccgg
     3541 gccacggcgg aggcgtcttc ctgacgccgg gacacccaag ccccgttccg tgaggccgtt
     3601 tccaaaccct ttgcgagata aaggcaaaac ttcggcttcg ttcgactatg ccctgagtac
     3661 tgagatatcg caagcgcgcg cgctggaatt gaaaaaaacc ggtgtatccg gcttgaatac
     3721 ccgtccgggg gccagttccc ggcccctgcc ccggcaccgt cgagcgtacc gcaggttggg
     3781 tacgcgcgcg aacctttggt tagtgcgatg acgaccgctg cggaaccgct gcctgccgaa
     3841 ctgccgcttc ccggcggagt cataggctcc gtcgtgcgcc tgagcgcgtt cctgtccgcc
     3901 acctgcctgg cgccccctgc ctatcttcat cgaatccagg gccgtctcgc ctcggttcga
     3961 gccctgggcc ttggtgtttc ccgggaagat tacgttcgaa tcccgattca aagcttcctc
     4021 ctggagcacc ccggcgtcgg ccacgttctg atcgacaccg gcttccatcc gtccgtcgcc
     4081 gtcgatccaa agaagaacct cggtcgcctc ggcactttgg ccttcagcgg tatcgagatg
     4141 gacgccgccg acgcggtccc ggcgcgccta cgcgcgatgg ggatcgatcc gcacgacgtg
     4201 ggtgttgtgg ttatgacgca cctccactcc gatcacgcga gcgcgatctc cgaattcggc
     4261 ggagcgacgt tcgtgttcag ccgcgcggag tgggaggcgg ccggtgagcc cggcggtacg
     4321 cgccggggct atgtgaagag ccagttcgac cacgcgttcg actaccggac gatcgacttc
     4381 gactccccgg aagtcgactc gttcgcgacc ttcggccgcg cggtggacct gttcggcgac
     4441 ggcagcgtca ggctcgtata cacgcccggg cacacacgcg gccacgtctc ggtgatcgcg
     4501 cggctggccg accgcgaggc gctcgtcgcc ggcgacgcga tctacacgat gcggacgctg
     4561 cgcgaggggg cggtcccgtg gctcacccag gacgagcacc gcttcaagcg ttcgctacgc
     4621 gagatccagc tctatgccaa gcagacaccg ggcgcgctca tcgtgccggg ccacgacctc
     4681 gaagcgttca gggccgccga gcgggagctc agcgaggtac cgggcggcgc gtagcggctt
     4741 cagccgcgtc catcgccttg cgaccggcgc ggcgtgcggc acgcgaggcg cgcacggcac
     4801 gcttgcgccc gccctcctgc acgccgtagt agagcttctc gacctcggcg aacacgatgg
     4861 tcgccgccgc ctgcgcctgc tcgcgactgg ggttctcgac gggctcgaag cggatcgcat
     4921 cgccgtactg caccgtgacc ttcggaaact gcccgcgctt ccaattgcgg gccttggcgg
     4981 atccgacgat cgccgtcggc acgaccggca cgccggtctc gagcgcgagc cgccccacgc
     5041 cgggcttcgc ctcgcccaga cgaccgctcc gcgacctccc gccctcgagg tacatgagca
     5101 cgatgtcgcc ccggtcgagc accttgcgcg cggtctcgaa cgacttctcg tcgcgctggc
     5161 cgcggcgcac cgggaacacg ccgccgtgcg tgtagatgaa ctgcatcggc cgggcgaaca
     5221 gctgcgactt cgccatgaac tgcaccttgc ggcgcaggta cacggcgacg aagaagtgat
     5281 cgaggaagga aaagtggttc ggcgcgacga tcgccgcccc gtggctcggc accttgtcag
     5341 tgtcgatcgc ccgcgtccgg tagacgagca ccatgatcgg cgtgagcacc aggcggcaga
     5401 agtcgtagat ccagtcgggg cgccgccggc gagcccgctc gtggaagcgg tcgaagtact
     5461 cggcggggcg cggatccttg tacacctggg gcttcactac tgggggttcc tacccgccct
     5521 gtggggattc gttactcgac tggtcccagc cgcggctgcg ctcgagcgcg cgatgccagc
     5581 cgtcgaggag ggactcgcgc tcgtcagccg acatgcgagg ctcgtatcgc gcgcgctctc
     5641 gccagatcgc ctggacgtcg tcctcggtcc aggcgccgac cgcgacgccc gccaggaagg
     5701 cggcgccgag cgcggtcgtc tccgacacct ccggtaccag gaccggcacc cctaggacgt
     5761 cggcctggaa ctccatcagc cagccgttca ccgcggcgcc gccgtcggca cgcagctcct
     5821 cgagctcctt tcccgcggcc cgctccgtgg cacggaccgc gtcgaccgtc tggtaggcga
     5881 tcgactcgag cgccgcgcgc gcgaggtgcg cgcggccgct cccgcgcgtg aggcccacga
     5941 tcgtgccgcg cgcatagggg tcccagtgcg gtgagccgag gccagtgagc gccgggacga
     6001 agtagacgcc gtcgttcgag cgaagcgagc gagcgagcgg ctcggtctct gccgcctccg
     6061 agatgatccc gagcccgtcg cgtagccact gcaccgccgc gccggtcacg aagatgctcg
     6121 cctcgagcgc gtagtccacg cgcccattcg cgccccaagc cacggtggtg agcagcccgt
     6181 cgggcgggtc gggcgcgtcc gtacccgcgt tctgcagcac gaagctgccc gtgccgtacg
     6241 tgttcttgcc gaggccgact tgcaggcagg cctggccgta gagcgccgcc tgctgatctc
     6301 cggccacgcc ggccaccggc acgtgaccgc cgaactcgct cgtgtggccg aacacgcccg
     6361 agctcgggcg agcgtcggga agcgactcgg cgtcgacgcc gagcagtccg cacagctcct
     6421 cgtcccagac caggtcgtgg atgtcgaaca gcagcgtgcg cgaggcgttc gtgtaatcgg
     6481 tggcctggtg gcctgtgagc ttgaagatca gccacgagtc gatcgtgccg aagcaggcgc
     6541 tcgggtccac accggcctcc ttgatcagcc actcgatctt cgtgcccgag aagtaggggt
     6601 cgagcacgag gccggttcgc gagcggaaca gctcctcgtg gccctgctcg cgaagctccg
     6661 cgcagcgcgc ggcggtgcgg cggtcctgcc acaccagcgc ccggtggagc ggctcgccgg
     6721 tgatcggctc ccaggccacg accgtctcgc gctggttggt gatgccgatc gcgtcgaggt
     6781 cccggccgga gatgccggcc gcgtcgagag cctcgtgcgc gacccggcgc gtcacctccc
     6841 agatctcgtg cgcgtcgtgc tccacccagc ccggtcgcgg gaagtgctgc tggaactcgg
     6901 agtaggccct gccggccacg cgcgcctggt cgtcgaagac gatgcacttg gtgccagtgg
     6961 tgccctggtc gatcgcgagg atcatcggcg cttagcgtaa gcgcgcgcga tgtcccggag
     7021 ctggcgaccg cgatgtgcga aaccggcggc ggttcggccc gtggcgcgat gctcgagctc
     7081 cacctccacc tccaccacgc gcatgccggc tcgcagcgcg tcgatgttca tgccggtctc
     7141 catgccgaag ccgcgggcga acggcagcaa atgctccacc agctcgccgc ggagcgcgcg
     7201 ctggcccgac agcggctgat cgagctcgcg cccgcagcgc cttcggaccg cccagcgcgc
     7261 gaagccgagc gcgatgccga agccgccgcc cacgcgcctg aggaacgcgg cgaccgccac
     7321 gtcggcgatg tcgtcctgga ccgcgtgcac aagcgcgatc agcttctctg cggtgcgccc
     7381 gaggtcgccg tcgcacagca gcagcacctc gggcgggtcg ccggcggcga gcacgcgctc
     7441 gaccgcctcc gtcatcgccc ctcccttgcc gatgtcgctg ccggtgctgt ggacttccgc
     7501 gccggccgcg cgcgcgatcg cggccgtctc gtcggtggag ccgtcgtcgg ccaccagcag
     7561 cagcgcgtcg gggaacgtcc ggcgcagcgc cgcgagcgtg tcggcgatgc ggtcggcttc
     7621 gtttcgagcg gccacgaccg ctgcgagttc cagtcgcacg ggcgagtatc gtgccgcaaa
     7681 cccgcgaccc aggaggaaca cggttgcctg acgttgaggc tcacatcacc ggcacggtct
     7741 ggaagatcga gtgccagatc ggccaggagg tcgaggaggg cgacacgctc gtgatcctcg
     7801 agtccatgaa gatggagatg ccggtggagg cggaggacga aggcgtggtc tcagagatcc
     7861 gctgcgaaga aggtcagtcc gtgtcggagg gtgacacgct cgtggtcctg gagtgagccc
     7921 cacgcaggag cttgcgtcgg gcaagctgct gctcgacgag cccgcggagc gggtggcgcg
     7981 tctgcggatt tcgaaccccg ccaagcgtgg tgcgctcgac cacgagatcc tcgacacgct
     8041 cgcggagacg gtcgcccatc tcgacgcccg ctgcctgatc gtgaccggtg acggccacgt
     8101 gttctccgcc ggctacgaca tcggcaactt cgacgacggc aacttcgagg aggcggccga
     8161 gaagctcgtc gcccacccgt tcacggcggc gatcgaggcg ctcgacgcct acccgtaccc
     8221 cacgctcgcc gccgtcaacg gccatgcgat cggcggcggg ctcgagctcg cggtcacgtg
     8281 cgacatccgc ctcgccgcga gcggtgtccg catgggcatg ccccccgcga agatcggcct
     8341 gatctactca cacaccgggc tgaagaagtt catcgacgcc tgcggcgtgg cgaacacgaa
     8401 cgagctcttc cacgtggggc gcaacgtgga tgccgagcgc ggccggcaga tgggcctcgt
     8461 gaaccaggtc ctggagcccg aggagctcga gcgcgccagc gtggagatgg cggccgagat
     8521 cgctcgcaac gcgccgctct cgctcgccgg caacaagcgc gtgatcagga cgctgcgcga
     8581 ctacccgaca cggcttcccg ctgaggtgga gcggcagctc gtggagctgc gcgagtcgtg
     8641 cttccgcagc gaggacttcc gcgaggggat ccgcgcgttc gcggagaagc gccaggccga
     8701 ctggaagggc cgctgagctg aactagggcg agggggccgg gtcaggactc ggctcgggcg
     8761 ccgggtcggg cctcggctcg accgggatca ctggcacgct cggcttccgt ggcgccggct
     8821 tgggcgcggg agccggccgt gacggcgcgc ggtacaccgg gggccgata
//
LOCUS       DASVXS010000072        10732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_300621, whole genome shotgun sequence.
ACCESSION   DASVXS010000072 DASVXS010000000
VERSION     DASVXS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300621"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /gene="ftsH"
                     /locus_tag="VF752_04155"
     CDS             complement(<1..1983)
                     /gene="ftsH"
                     /locus_tag="VF752_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8854771.1"
                     /translation="MPDQPPSRESQQRRPDEHPRPWRVEGHPDEPETPQQTQTRWRPP
                     GGRWFWPILVALLAVNFVVSLTTTKGPTRISVPYTFFRQQVDQGNVKEITSQSDKIQG
                     TFKQKVKYPPTNGDTSDKFKTVKPAFDDPGLLQLLISKGVVVNAHPLDEGTPWWQTLL
                     FGFGPTLLLVGLFILFARRAAAGAGGFGGLTRSRARLYEPSKQRVTFKDVAGIDEAED
                     ELIEIVDFLRNPNRYRRLGGTIPKGVLLSGPPGTGKTLLARAVAGEANVPFFSLSASE
                     FVEIVVGVGASRVRDLFQQAKQVAPSIIFIDELDAIGRSRGAGAGFGGHDEREQTLNQ
                     ILTEMDGFSGTEGVIVLAATNRAEILDPALLRPGRFDRRVIVNPPDQAGRRKILEIHT
                     RSVPLADDVDLDAIASSTPGMVGADLRNLVNEAALTAARRNHDRVQAQDFTDALEKIV
                     LGAARRITLTQEDRERTAYHESGHAVLGMLQPGADPVRKVSIVPRGRALGVTFQAPDV
                     DRYGYGAAYLRGRMIGALGGRAAEEIIYGDVTTGAESDLEQVTAIARQMVGRWGMSDA
                     IGPVSVLPRDEDAPLIPGANSAADATRELVDAEVRRIIDECYDIAVSTLRDNRAHLES
                     LSRALLEHETLDEGDAYRAAGIEPPARDGGPPAAAPE"
     gene            complement(2035..2895)
                     /locus_tag="VF752_04160"
     CDS             complement(2035..2895)
                     /locus_tag="VF752_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX8854772.1"
                     /translation="MLIGGHVSTAGGLVNAHARGIEIGASAIQVFNQSPRMWRPTRWK
                     DDDIAEFKELMANGPIESVVIHAVYLINPATKDKEMRKKSLHSLVHAASMGESIDADG
                     VVIHPGSTVGEPHAAAIKRAGAAFKHALEETDRCPILLEDTAGAGNTLGRDFGELAAI
                     IDKAGGSKRLGLCLDSCHLFASGFDVSTPEGLAKVLDDFDAEIGLERLKCLHVNDSQT
                     GLGSNRDRHAPLGTGKLGEEGCATFLSEPRFEDLPVLFEGPGFAGKAPAKEDVDCARK
                     LREQGLKSRA"
     gene            2914..4179
                     /gene="gltX"
                     /locus_tag="VF752_04165"
     CDS             2914..4179
                     /gene="gltX"
                     /locus_tag="VF752_04165"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX8854773.1"
                     /translation="MRVRFAPSPTGALHIGGARTALYNWLLARGNRGTFVLRIEDTDR
                     ERSTPENVAQIIDALEWLELDWDEGPYSQAERRPRHQEVIEQLKASGDAYEEDGATRL
                     KVPDEGQTVVHDAIRGEIVFNHADIDDFTIARSDGSPLYNLAVAVDDVDMGITHVIRG
                     DDHISNTPRQLMIMNALGHEPPVYAHLPLLYGPDGKKLSKRHGAASVQELRAAGYLPE
                     AVRNYLALLGWGYDDTTTFFTTGELVERFSLERVSKSSAVFDEQKLRWMNGRYLREMG
                     TLELKDKIERLIGRAVPREAVEISQEKMQTLADFWPLAGFLVERREIDPKAWEKVMRD
                     GAADNLARAREALAGTDPFDTEHVEQSLRGLVEALGVKPKDVFQPIRVAIAGTTVSPG
                     IFESVAALGRDETLSRLDAALERAGTNGR"
     gene            4326..5390
                     /locus_tag="VF752_04170"
     CDS             4326..5390
                     /locus_tag="VF752_04170"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX8854774.1"
                     /translation="MARARAEGVRGPQPPQPDGHGRRLTAAFEALEGFPALVESRNRL
                     LRLAREERSSVGEMVAAVESDVSLVIAVLRLANRAPGAKKGKVTSIPKAIEILRPSGV
                     EALASRTAVFDFFERTPGWDAQPERFRLHSVATQRAAERLAREIGYEDRDELLVSALL
                     HDIGQLVLVHAYPGFPGQVHGDARTPEERVHRERRELGVDHALVGGVLARRWGLPNRL
                     AAAIERHHSDEAEGEAAIIRLADMLAHYTHGQPVEPAEMLKVSRSLGLSAESLRAVMY
                     ELPYGGGQSRRNVEPCPLSNREVDVLKRLAEGKVYKQIALELSLSTSTVRTHLHNTYT
                     KLGAVDRAQAVLVATERGWI"
     gene            complement(5395..6594)
                     /locus_tag="VF752_04175"
     CDS             complement(5395..6594)
                     /locus_tag="VF752_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8854775.1"
                     /translation="MIHLGRPPIASREPVVIFAAIRLAVALVAGIAVVASGFPYGGML
                     ALGIYAVGLPWAIGIYVLARRNPSAALHPLVAAGDLIVLAVIELLVPETYGAVRFFAL
                     FLIAAHGQFQGIRGGMLVAAFGVILLLPIAAVTDPPVIGEDLLAYYESLFVVCAFGSA
                     WVGANLRTAETAGRLRARELTRRMIETENDVRRRVADAIHDGPVQELTSVDMMLSAAE
                     AAAERGDSERATQLIEEARAITARNIDALRAELVGLGPQAYEGLSFEDALMQSVPKWS
                     NRYDVHIDVDVDSVDAPPELSGTLFQITQEAVVNVGRHADATRVAIRLSDDGDAVELR
                     VEDDGHGFYVDPLGAGAVGHIGLASMRERAELINGHLTIDSSPDGAVVRVHVPLHHGS
                     SRRTRFG"
     gene            complement(6591..6956)
                     /locus_tag="VF752_04180"
     CDS             complement(6591..6956)
                     /locus_tag="VF752_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX8854776.1"
                     /translation="MTLTSRTALWLPPFVLMGVIFFFSAQPDLNSGLGLADLIGRKII
                     HASEYALLTFLWWRAFVRTSFRRAAPLIGFSLAVGYAGTDEFHQTFIHGRDGSPVDVC
                     IDAFGAGLCALFLHRRTRR"
     gene            6983..7384
                     /locus_tag="VF752_04185"
     CDS             6983..7384
                     /locus_tag="VF752_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8854777.1"
                     /translation="MLTITTKSPYAVSALIELAKCGASVPVPIGTLAERRGIPVQFLE
                     SLFAALRRAGVLRSQRGVRGGYSFERSPSEISVLEVVELLEGDLSAEAAKMGEIWDEA
                     ASALRGVLGGVTIADLAEREARESGALMYHI"
     gene            7901..8152
                     /locus_tag="VF752_04190"
     CDS             7901..8152
                     /locus_tag="VF752_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854778.1"
                     /translation="MTGPLYHLDPRSPTCEPTITNAWEVVERGRATVKATFSDRSETN
                     LLFVYDTDELSFTMAELIGLTAAEARTLRHKRDVAYLRS"
     gene            8416..9468
                     /locus_tag="VF752_04195"
     CDS             8416..9468
                     /locus_tag="VF752_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX8854779.1"
                     /translation="MPETPLISLDHEELVIRRGRRTGAYTIVAVHSTALGPALGGCRM
                     WRYESSADGARDALRLSRAMTFKSAACGLDVGGGKGVICLEPGTPPTGTVRRDLLLDM
                     ADTINVLDGNYITAEDVGTSSRDMAVIAEGTKWVTGLDRRRGGSGDPSPYTAMGVEYA
                     IRACCENRFGSRDLKGRSVAVVGAGRVGSQLAKRLAKAGAKLIVADIDERQREAMEKL
                     GARWTDPSSAMLADVDVLAPCALGGVIDQVNVGLLRCRIVCGSANNQLAHEGLADDLA
                     ARGILYAPDFIANAGGIINVSLELGNYDPALAREGARHIEHTMSELLAIAEETGDTPL
                     AAAYRLARARLERARR"
     gene            9478..10425
                     /gene="cysK"
                     /locus_tag="VF752_04200"
     CDS             9478..10425
                     /gene="cysK"
                     /locus_tag="VF752_04200"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX8854780.1"
                     /translation="MSSGPRTSAIPANLADIVGGTPMVRMTRIDPEGGAEIVAKLEAY
                     NPAGSVKDRIGVAMIEAAEAEGRIEPGRTTIVEPTSGNTGIALAFVCAAKGYNLILSM
                     PEGMSREREGLLRLYGARVELTESLGGMTEAVQQAERMAKELGDVFIPDQFSNPANPE
                     IHRRTTAEEIWRDMDGSLDVLVAGVGTGGTITGAGERLKDRNPDLYVVAVEPAASPVL
                     SGGRPGPHKIQGIGAGFVPTVLNRDVIDEIIAVDDEDALDTARLASRTEGVLAGISGG
                     AALWAGIQLGRRPEFAGKRIVVIVPDSGERYVSTPFFAP"
     gene            10431..10628
                     /locus_tag="VF752_04205"
     CDS             10431..10628
                     /locus_tag="VF752_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX8854781.1"
                     /translation="MLDLTGVPCPMNWVRTKLALESLEPGEELSVALDDGEPIESVPR
                     SAREDGHEVTVQGNTVNIVKR"
BASE COUNT         1693 a         3670 c         3787 g         1582 t
ORIGIN      
        1 ctcgggcgcg gcggccggcg ggccgccgtc gcgcgccggt ggctcgatgc ccgcggcccg
       61 gtatgcgtcc ccctcgtcga gcgtctcgtg ctcgagcagc gcgcgggaga gcgactcgag
      121 gtgcgcacgg ttatcgcgca gggtggagac cgcgatgtcg tagcactcgt cgatgatgcg
      181 gcgcacctcg gcgtccacca gctcccgcgt cgcgtcggcg gcgctgttcg ccccggggat
      241 gagcggcgcg tcctcgtcgc gcggcagaac cgagaccggg ccgatcgcgt ccgacatgcc
      301 ccagcgcccg accatctggc gtgcgatcgc ggtcacctgc tcgagatcgg actcggcgcc
      361 ggtcgtcacg tcgccgtaga tgatctcctc ggccgcacgc ccgccgagcg caccgatcat
      421 gcgcccgcgc aggtaggccg cgccgtagcc gtagcggtcc acatccggcg cctggaaggt
      481 gacgccgagc gcgcggccgc gcggcacgat cgacaccttg cgcaccggat ccgcgcctgg
      541 ctgaagcatg ccgagcacgg cgtgaccgga ctcgtggtag gcggtccgct cgcggtcctc
      601 ctgcgtgagc gtgatgcggc gggcggcgcc cagcacgatc ttctcgagcg cgtcggtgaa
      661 gtcctgcgcc tggacgcggt cgtggttacg gcgtgccgcc gtgagcgccg cctcgttcac
      721 gaggttgcgc aggtcggcgc ccaccatgcc gggcgttgag gaggcgatcg cgtcgagatc
      781 gacatcatcc gcgagcggca cactccgcgt gtggatctcg aggatcttgc gtcggccggc
      841 ctgatccggt gggttcacga tcactcggcg gtcgaagcgc cccggccgca gaagcgccgg
      901 atcgagaatt tccgcccggt tggtagcggc cagcacgatc acgccctctg tgcccgagaa
      961 gccgtccatc tcggtgagga tctggttgag cgtctgctcg cgctcgtcat gcccgccgaa
     1021 gccggcgccg gctccgcggg agcgcccgat cgcatcgagc tcgtcgatga agatgatcga
     1081 cggcgccacc tgcttcgcct gctggaacag atcgcgcacg cggctcgcgc cgacgcccac
     1141 cacgatctcc acgaactcgg acgcggacag cgagaagaac ggtacgttcg cctcaccagc
     1201 aacggcgcgc gcgagcagcg tcttgccggt gccgggcggt ccggacagca gcacgccctt
     1261 cgggatcgtg ccgccgagac gccggtagcg gttcgggttg cgcaggaagt ccacgatctc
     1321 gatgagttcg tcctcggcct cgtcgattcc ggcgacgtcc ttgaaggtga cgcgctgctt
     1381 gctcggctcg tacagccgcg cgcgcgagcg cgtgagcccg ccgaagccgc cggcgccggc
     1441 cgcggctctt cgcgcgaaga ggatgaacag gccgaccagc agcagcgtgg ggccgaagcc
     1501 gaacaagagc gtctgccacc acggcgtgcc ctcgtcgagc gggtgcgcgt tcaccaccac
     1561 acccttcgag atcaggagct gcagcaggcc ggggtcgtcg aacgccggct tgaccgtctt
     1621 gaacttgtcg gaggtgtcgc cgttggtggg tgggtacttg accttctgct tgaaggtgcc
     1681 ctggatcttg tcgctctgcg acgtgatctc cttcacgttc ccctggtcca cctgctggcg
     1741 gaagaacgtg tagggcacgc tgatgcgagt cgggcccttg gtggtggtca gcgagacgac
     1801 gaagttgacc gcgagcagcg cgaccaggat cggccagaac cagcgcccgc cgggtggccg
     1861 ccagcgcgtc tgagtctgct gcggcgtctc gggctcatcc gggtgccctt ccacccgcca
     1921 cgggcggggg tgctcatccg ggcgccgctg ctgagactcg cgggaggggg gctgatcggg
     1981 catggccttc cagccacatt actcagccgg ctcaccgtgc acagggggtt tcgctcaggc
     2041 gcggctcttg agaccctgct cgcgcagctt gcgggcgcag tccacgtcct ctttcgcggg
     2101 cgccttgccg gcgaatccgg ggccctcgaa caacaccggg aggtcctcga acctcggctc
     2161 cgagaggaac gtcgcgcagc cctcctcgcc gagcttgccc gtgccgagcg gagcgtggcg
     2221 gtcgcggttc gagccgaggc cggtctgcga gtcgttcacg tgcaggcact tgaggcgctc
     2281 gaggccgatc tcggcgtcga agtcgtcgag caccttcgcg agcccctcgg gcgtgctcac
     2341 gtcgaagccg ctcgcgaata gatggcacga gtcgaggcag agccccagcc gcttcgatcc
     2401 gcccgccttg tcgatgatgg cggccagttc gccgaaatcg cgtccgagcg tgttgcccgc
     2461 tccggcggtg tcctcgagca gaatcgggca gcgatcggtc tcttcgagcg cgtgcttgaa
     2521 cgccgcgccg gcccgcttga tcgcggctgc gtggggctca ccgacggtcg atccggggtg
     2581 gatcaccacg ccgtcggcat cgatcgactc acccatcgac gccgcgtgga cgagggagtg
     2641 aagcgacttc ttgcgcatct ccttgtcctt ggtcgccggg ttgatgaggt agacggcgtg
     2701 gatcaccacg gactcgatcg gcccgttggc catgagctcc ttgaactcgg cgatgtcgtc
     2761 gtccttccag cgcgtgggcc gccacatccg tggcgactgg ttgaacacct ggatcgcgct
     2821 cgctccgatc tcgatgcccc gggcgtgggc gttcactagc ccgcccgccg tcgagacgtg
     2881 gccgccgatt agcatcgagg tgtggccgat tccatgaggg tgaggttcgc gccgtcgccg
     2941 acgggcgcgc tgcacatcgg cggggcgagg acggcactgt acaactggct tctggcccgc
     3001 ggaaaccgcg ggaccttcgt gctgcggatc gaagacaccg accgcgagcg ctccacgcct
     3061 gagaacgtgg cgcagatcat cgacgcgctc gagtggctcg agctcgactg ggacgagggg
     3121 ccctactcgc aggccgagcg ccgcccgcga caccaggagg tgatcgagca gctgaaggcc
     3181 tccggggacg cctacgagga ggacggcgcg acccgcctga aggtcccgga cgagggccag
     3241 accgtggtcc acgacgcgat ccgcggcgag atcgtcttta accacgccga catcgacgac
     3301 ttcacgatcg cgcgctcgga cggcagcccg ctctacaacc tggcggtcgc ggtggacgat
     3361 gtggacatgg gcatcacgca cgtgatccgc ggcgacgacc acatctcgaa cacgccgcgc
     3421 cagctgatga tcatgaacgc gcttgggcac gagccgccgg tctacgccca cctgccgctg
     3481 ctctacggcc cggacggcaa gaagctctcg aagcgccatg gcgccgcatc ggtgcaggag
     3541 ctgcgcgcag ccggctacct gccggaggcc gtgcgcaact atctggcgct gctcggttgg
     3601 ggctacgacg acacgaccac cttcttcacc acgggcgagc tggtggagcg cttctcgctc
     3661 gagcgcgtgt cgaagtcgtc cgcggttttc gacgagcaga agctccgctg gatgaacggg
     3721 cgctacctgc gggagatggg cacgctggag ctgaaggaca agatcgagcg gctgatcggg
     3781 cgtgcggtgc cgcgcgaggc ggtcgagatc tcgcaggaga agatgcagac gctcgccgac
     3841 ttctggccgc tcgccggctt cctcgtggag cggcgcgaga tcgacccgaa ggcctgggaa
     3901 aaggtcatgc gcgacggggc cgcagacaac ctcgctcgcg cgcgcgaggc tctggcaggc
     3961 acggacccgt tcgacaccga gcacgtcgag cagtcgctgc gggggctggt ggaggcgctc
     4021 ggcgtgaagc ccaaggacgt cttccagccg atccgtgtgg ccatagcggg gacaacggtt
     4081 tcacccggaa tcttcgagtc ggttgccgct ttggggcgcg acgagactct ttcgaggctg
     4141 gacgccgcgc tcgagcgcgc gggcacgaac ggccgatagc cacaatttcc ccggaaactg
     4201 cggcaaaacg cgagaaatct cgcatcgggc ctcaaccttt cgtccacccc tgccgatagc
     4261 caaggtgcag gctcagtccc ctgacccagg caggcattca gaacttggaa gcagcaactt
     4321 ctcagatggc acgcgctcgc gcggagggag tgcgtggccc ccagcctccg cagccggacg
     4381 gccacggccg caggctcacg gccgcgttcg aggccctcga gggcttcccg gccctcgtcg
     4441 agtcgcgtaa ccggctgctg cgcctcgcac gcgaggagcg ttcctcggtc ggcgagatgg
     4501 tcgccgccgt ggagtccgac gtgtccctcg tgatcgccgt gctgcgcctg gcgaaccgcg
     4561 cccccggcgc gaagaagggc aaggtcacga gcattccgaa ggcgatcgag atcctgcgcc
     4621 cgtcgggcgt cgaggcgctc gcctcgcgca ccgcggtgtt cgacttcttc gagcgcaccc
     4681 ccggctggga cgcccagccc gagcgcttcc gcctgcactc ggttgccacc cagcgcgccg
     4741 ccgagcggct cgccagggag atcggctacg aggaccgcga tgagctgttg gtgtccgcgc
     4801 tgctccacga catcggccag ctcgtgttgg tgcacgccta tcccggcttc ccggggcagg
     4861 tgcacggcga tgcccgcact ccggaggagc gcgtgcaccg cgagcgccgc gagctcgggg
     4921 tcgaccacgc gcttgtgggc ggcgtgctcg cccgccgctg gggcctgccg aaccggctcg
     4981 ccgcggccat cgagcgccat cactccgacg aggccgaggg cgaggccgcg atcatccgcc
     5041 tggccgacat gctggcccac tacacgcacg gccagccggt cgagccggcc gagatgctca
     5101 aggtgagccg ctcactcggc ctgtcggcgg agtcgctgcg cgcggtgatg tacgagcttc
     5161 cctacggtgg cggccagtcg cgccgaaatg tggagccgtg cccgctctcg aaccgcgagg
     5221 tcgacgtgct caagcgcctc gccgagggca aggtgtacaa gcagatcgcg ctcgagctga
     5281 gcctctccac gagcacggtg cgcacgcacc tccacaacac ctacacgaag ctcggcgccg
     5341 tcgatcgcgc acaggccgtc ctggtcgcaa ccgagcgcgg ctggatctag gccgctagcc
     5401 gaagcgggtc cgccggctgc tcccgtggtg gagcggcacg tgcacacgca cgacggcgcc
     5461 gtcgggcgag ctgtcgatgg tcaggtgccc gttgatcaac tcggcacgct cgcgcatgct
     5521 ggcgaggccg atgtggccga cggccccggc gccgagtggg tccacataga agccatggcc
     5581 gtcgtcctcg acgcgcagct cgacggcatc accgtcgtca gagaggcgga tcgcgacgcg
     5641 cgtcgcatcg gcgtgccggc ccacgttcac gacggcttcc tgcgtgatct ggaagagcgt
     5701 gccgctcagc tcgggcggcg cgtcgacgct gtcgacgtcc acgtcgatgt gcacgtcgta
     5761 gcggttcgac cacttcggga ccgactgcat cagcgcgtcc tcgaacgaga ggccctcgta
     5821 agcctgcgga cccaggccga ccagctcggc gcgcagcgcg tcgatgttgc gcgcggtgat
     5881 ggcgcgcgcc tcttcgatca gctgtgtggc gcgctcgctg tcgccgcgct ccgcggccgc
     5941 ctctgcggcg ctcagcatca tgtcgacgct agtcagctcc tgcaccggcc cgtcgtggat
     6001 ggcgtcggcc acgcgccggc gcacgtcgtt ctcggtctcg atcattcggc gggtgagctc
     6061 tcgcgcgcga aggcggcctg cggtctcggc cgtgcgcagg ttcgcgccga cccaggccga
     6121 gccgaacgcg cacaccacga acagcgactc gtagtacgcg agaaggtcct cgcctatgac
     6181 tggcgggtcc gtgaccgccg cgatcggcaa caagaggatc accccgaacg cggcaaccag
     6241 cattccgccg cggatgccct ggaactggcc gtgtgcggcg atcaggaaca gcgcgaagaa
     6301 gcggaccgcg ccgtaggtct ccggcacgag cagctcgatc acggcgagca cgatcaggtc
     6361 gcccgccgcg acgagcggat gcagcgccgc ggacggatta cgccgcgcga gcacgtagat
     6421 gccaatcgcc cacggcagac ccacggcgta gatcccgagc gcgagcatgc cgccgtaggg
     6481 gaagccgctc gccaccaccg cgatgccggc gacgagcgct accgcgaggc gtatcgccgc
     6541 gaatatgacg acgggctctc gcgaggcgat cggcggccgg ccgagatgga tcatcttcgt
     6601 gttcgcctgt gaaggaacag cgcgcagagc ccggcgccga aggcgtcgat gcagacgtcg
     6661 accggtgagc catcccggcc gtggatgaag gtctggtgga actcgtcggt tcccgcgtag
     6721 ccaaccgcga gcgagaaacc gatgagcggc gccgcgcggc ggaagctcgt gcggacgaaa
     6781 gcacgccacc acagaaacgt gagcagcgcg tactccgatg cgtggatgat cttccgcccg
     6841 atcaggtcgg ccagaccgag cccggaattc aggtccggct gcgccgagaa gaagaagatc
     6901 acgcccatca gaacgaaggg cgggagccag agcgccgtgc gggaggtcag cgtcacgtcc
     6961 ataacctaga ctcgccgggc acgtgctgac cattacgacg aaatccccgt acgcggtaag
     7021 cgcgctcatc gagctggcga aatgcggggc gtcggtgccg gtgccgatcg gcacgctcgc
     7081 ggagcgtcgc gggatcccgg tccagttcct cgagagcctg ttcgcggcgc tgaggcgcgc
     7141 aggggtgctg cgctcgcagc gcggcgtgcg cggcggctac tcgttcgagc gctcgccgtc
     7201 cgagatctcc gtgctcgagg tggtggagct gctcgagggc gacctctcgg ccgaggccgc
     7261 gaagatgggc gagatctggg acgaggccgc gtccgccctg cggggcgtgc tcggcggcgt
     7321 cacgatcgcc gacttggcgg agcgtgaggc gcgcgagtcc ggcgcgctga tgtaccacat
     7381 atgagttgct ccgcttccta cagcgctttg gcgccctcgg gctgcagaca accggattcg
     7441 cggcagctac cacgaccacc acgctgtcgg atgggtggcg atcgggtgat ggctcgtccc
     7501 ggcgcacgtt ggctcggtcg gttcgacacc aatcggcaga tctgacccgc ccgtcgacag
     7561 tctccggcgc ggctgaattt gggcgtttct cggctccgcg cccggaaaga attcgcgtag
     7621 ggcgccgggc ttcgcatctg gcaaaccgcc accgcccgac gctttcccgc ggtggtggct
     7681 ggggaggacc cggcgcccgg aaacagcggc gagtctggcg tggacagctt cagatggcag
     7741 cctccgggtc gcgcatgagc gttgtgattc gcaccgcggc tggctcggac tgaccctcac
     7801 ggacacccca gcaaacgctg ggggaaagcc tggggaccct cccattgtgc cgccctgcaa
     7861 gcggatccaa gcttcgaccg caagcaagaa ggtcactcag atgactggtc cgctctatca
     7921 cctcgatccg cgatcaccga cctgcgagcc gacgatcacg aacgcgtggg aggtcgtaga
     7981 gcgcggccgc gcgacggtca aggccacgtt ctccgatcgc tccgagacga acctgctgtt
     8041 cgtgtacgac acggacgagc tgagcttcac gatggccgaa ctgatcggcc tcaccgctgc
     8101 ggaggcccgc acgctgcgtc acaagcgcga cgtcgcgtac ctgcgctcgt gagaaccgtt
     8161 ccgcttgcca atgtggccgg gtggtggctc gccagcggca agtcgggaaa gatgcgactt
     8221 gcacaaaacc gttccgggag ccggttcgat aacgcacttg cttggtcctg gccgacgaac
     8281 acgagtccac ctgccaaccg acgccgtaac cggagtcgac gcggcgccgt aaccgcactc
     8341 gcaaccgtgc gggccacgag cgccgcgtgc cgccgtccag ctgcccggac cgatagaaca
     8401 cggggcggag gacgtttgcc agagaccccg ctgatcagcc tcgaccatga ggagctcgtc
     8461 atccggaggg gccggaggac gggtgcctac accatcgtcg cggtgcactc gacggcgctc
     8521 ggcccggcgc tcggcggctg ccgcatgtgg cgctacgaga gctcggccga cggcgcgcgc
     8581 gacgccctcc gcctctcacg cgcgatgacc ttcaagtccg ccgcctgcgg cctcgacgtc
     8641 ggcggcggta agggggtgat ctgcctcgag cctggcacgc cgcccacggg gacggtgcgg
     8701 cgcgatctgc tgctcgacat ggcggacacg atcaacgtgc tcgacggcaa ctacatcacc
     8761 gctgaagacg tgggcacgag ctcgagggac atggcggtga tcgcggaggg gaccaagtgg
     8821 gtcaccggcc tcgaccggcg ccgcggcggc agtggcgacc cgagcccgta caccgcgatg
     8881 ggcgtggagt acgcgatacg cgcctgctgc gagaaccgct tcggcagccg cgacctcaag
     8941 ggccggagcg tggcggtcgt cggcgccggg cgggttggat cgcagctcgc gaagcgcctt
     9001 gcgaaggccg gcgcgaagct gatcgtggcc gacatcgacg agcggcagcg cgaggcgatg
     9061 gagaagctgg gcgcgcgctg gactgacccg tcctcggcca tgctcgccga tgtcgacgtg
     9121 ctcgccccgt gcgcgctcgg cggcgtgatc gaccaggtga acgtggggct gctgcgctgc
     9181 cggatcgtgt gcggctccgc caacaaccag ctcgcgcacg agggcctcgc ggacgacctc
     9241 gcggcgcgcg gcatcctcta cgcacccgac ttcatcgcca acgcgggagg gatcatcaac
     9301 gtctcgctcg agctcggcaa ctacgacccg gcactcgcgc gcgagggcgc caggcacatc
     9361 gagcacacga tgtccgagct gctcgcgatc gcggaggaga ccggcgacac gcccctcgcg
     9421 gccgcctacc ggctcgctcg tgcccggctc gagcgggcaa gaaggtaggt tcaaagggtg
     9481 agcagcggcc cccgcaccag cgcgatcccg gccaacctgg cagacatcgt cggcggcacg
     9541 ccgatggtgc gcatgacgcg catcgacccc gagggtggcg ccgagatcgt cgcgaagctc
     9601 gaggcctaca acccggccgg gagcgtgaag gaccggatcg gggtggcgat gatcgaggcc
     9661 gccgaggccg agggccgcat cgagcccggg cgcaccacga tcgtggagcc gacgtcgggg
     9721 aacaccggga ttgcgctcgc gttcgtgtgc gccgcgaagg gctacaacct gatcctctcg
     9781 atgcccgagg ggatgagccg agagcgcgaa gggctgcttc gcctctacgg ggcgcgcgtg
     9841 gagctgacgg agtcgctcgg cggcatgacg gaagccgtgc agcaggccga gcgcatggcg
     9901 aaggagctcg gcgacgtgtt catcccggac cagttctcga accccgccaa ccccgagatc
     9961 caccggcgca cgaccgccga ggagatctgg cgcgatatgg acggcagcct ggacgtgctc
    10021 gtagcgggcg tgggaaccgg cggcacgatc accggcgccg gggagcggct gaaggaccgc
    10081 aatcccgacc tctacgtcgt ggccgtggag cccgcggcct cgcccgtgct ctcgggcggg
    10141 cgccccggac cgcacaagat ccaggggatc ggcgcgggct tcgttccgac ggtgctaaac
    10201 cgcgatgtga tcgacgagat catcgccgtg gacgacgagg acgccctcga cacggcgcgg
    10261 ctcgcgtccc gcaccgaggg cgtgctcgca ggcatctcgg gaggcgcggc gctgtgggcg
    10321 gggatccagc tcggacgccg gccagagttc gcggggaagc ggatcgtggt gatcgtgccg
    10381 gactccggcg agcgctacgt gtccacgcct ttcttcgcgc cgtgaccccg atgctcgatc
    10441 tgacgggcgt gccctgcccg atgaactggg ttcgcacgaa gctggcgctc gagtcgctcg
    10501 agccgggcga ggagctgagc gtggcgctcg acgacggcga gccgattgag tcggtgccgc
    10561 ggtccgcgcg cgaggacgga cacgaggtga ccgtccaggg aaacaccgtc aacatcgtga
    10621 agcgatgagc ctctcggacc aggagctcga gcgctactcc cgccagctcg tgctgcccga
    10681 gtggagcggg gcagcgcaag agcagcttaa gagcgcgacc gtggtggtcg tc
//
LOCUS       DASVXS010000073        24064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_513357, whole genome shotgun sequence.
ACCESSION   DASVXS010000073 DASVXS010000000
VERSION     DASVXS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24064
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513357"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF752_04210"
     CDS             complement(<1..314)
                     /locus_tag="VF752_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854782.1"
                     /translation="MKRILVFAAALIALLALAGGPAVARDSDAPPGASPRWLPCEHWV
                     MFHWLPYDETRLYSLLGVHRATVLRWARDDQHRTLGDLARRRGWKPDALASALVRPWR
                     GRV"
     gene            376..1704
                     /locus_tag="VF752_04215"
     CDS             376..1704
                     /locus_tag="VF752_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX8854783.1"
                     /translation="MASLWRNWTGDQRCAPAEIAEPGSEAELAEVIARARARGLPVRA
                     VGSGHSFTDIACTDGVMVRLRRMNRLVHADPATGRVRVQAGITLRELGQRLAEHGLAL
                     ENMGDIDAQTLAGALATATHGTGARYGNLATQVASLRLMTADGLVAELSEDDDDRETW
                     LAARVSLGALGIVTEVELRCVPAFALHRIDEPRPLAETLERFHSLVEENEHFEFFAMP
                     YTDVAMCRKTNRTEQPPQPRERVAAYVQDVLLENRALDLFCRAGRAVPRAIPALNRRL
                     AGFFSRTELVDRSHRVHPNRRDVRFTEMEYAVPREAGPDAVRRIFDLIERRRLTVNFP
                     IEVRAVAADDAFLSPAHDRPTTYIAVHSYQGMEFESYFRGVEAIMDEHEGRPHWGKRH
                     YQSAPTLRERYPRWDSFQDVRARLDPDGVFANDYTRRVLGPVGAELRAPA"
     gene            complement(1701..2720)
                     /locus_tag="VF752_04220"
     CDS             complement(1701..2720)
                     /locus_tag="VF752_04220"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX8854784.1"
                     /translation="MTERQGGARTAIHETGYPALVLDRLARHTCRILDCEQSWIVIRD
                     RLDPRAAMAVAAHGVDEHTIAERVGTDEGVAGSVLAGFGSRQTAPFVIQHDGLGVSAC
                     APVSWEGSVRAALCVTSMRPGRRFGQRELEELSELAELASAALAHAEARERPDATVAA
                     RVDALAAAMDMRDGYTAQHSDDVVDLALEVGERLRLDRAALVELEFAARLHDVGKIAV
                     PDAILRKRGHLDDGEWEMMRCHPVWGAETLVRIPGLEVVATIVRFHHERWDGTGYPDG
                     LKGDRIPLASRIISACDAYKAMTSNRPYRSALRRDSALAEIEAGAGTQFDPLVVTELV
                     AVVRG"
     gene            complement(2816..4003)
                     /locus_tag="VF752_04225"
     CDS             complement(2816..4003)
                     /locus_tag="VF752_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEX8854785.1"
                     /translation="MSELDRYGRAFEGIDAPFAFVDLDAMWENATAMAARARGKPLRL
                     ASKSVRCRRLLRDVLDRDVGFRGLMTFTLAESLWLNELGFDDILLAYPTADREALAQL
                     TRIDGERPPALMVDSLEHLDFIEAAAGDPARPVRVCIEIDLSWWALGGRLKVGPKRSP
                     IRTPQQAQLLAREIARRPRLELVGLMGYEGHIAGVGDAPAGRRVRGAVIRRIQEASAR
                     EIAERRASVVEAVNAVALLEFVNGGGTGSIDRTAAEPAVTEVTAGSGLYAPTLFDAYS
                     SFSLRPAAMFALPVVRRPSRRTVTVLGGGYIASGAPGRDRLPSPHLPPGLRLDPMEGA
                     GEVQTPVSGAAAERLRVGDRVYFRHAKAGELCERFDRLYLVSGAELVGEVATYRGEGR
                     CYL"
     gene            complement(4000..5064)
                     /locus_tag="VF752_04230"
     CDS             complement(4000..5064)
                     /locus_tag="VF752_04230"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX8854786.1"
                     /translation="MSAPELHELVCVVHVHSIYSDGTGTVPEIADAAARAGADVVLLT
                     DHDTLSARDNGEEGWHGPVLLLVGTEISPPNRDHFLAFGINRLVSRRLSGPEIAAAVR
                     EAGGFGFAAHPFSHGSERFSRVEPMPFAEVTDDRVDGLELWSFLNDTAERLASLPAVA
                     RFIAAPRRVVDHPPAGNVAAWDAVCARRRMVAIAGTDAHQVGLRMRGRVPLRLMSYRR
                     SFSMLRTHALCEEPLSRDLDHDRTQVYSALREGRCYMAFDGAAPAHGFSFWAEGEAGT
                     LRMGEEAELGEGYELHARSPRHATLRLLRNGDELARNAGRAIDFEASEPGAYRVDARL
                     DLKGREVTWVLTNPVYLRAR"
     gene            5206..6309
                     /locus_tag="VF752_04235"
     CDS             5206..6309
                     /locus_tag="VF752_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854787.1"
                     /translation="MAVIESPEKIGIRPFHEFDDANDTWPRGERPDAIRDAAREFRGR
                     FKAQGEIHAVRTIDLVSAGYPTAFALAGAAKGLNPYINIRNRLVVVQFDDFDGQRRTL
                     VWEPTVPEGSVEAPFYAQLLERYGEFLSEKVFAKYFNTVGEALALCGLRPEDVDYVSF
                     DHLHVQDMRILMGTTEPVDGEPVPRPPFFPNAKFLCQRKEVDTLHSMHPMQWAWYVPH
                     GMDKVIDDNLVLVDGDVELGVGVALVWTPGHTDGNQSLCINTPDGIWITSENGVCADS
                     WHPRLSKIPGVRKYAEFYNREVVLNSNTLEDSIDQYDSMIKEKALADPNRRDPRFLNV
                     FPSSEMESFRRQWPVVPTFVYGGMNYGEIQRAA"
     gene            6341..6988
                     /locus_tag="VF752_04240"
     CDS             6341..6988
                     /locus_tag="VF752_04240"
                     /inference="COORDINATES: protein motif:HMM:NF006743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854788.1"
                     /translation="MQSGGRRALIGITGPPAAGKTTLMEALARRLRADGYAAAGVPMD
                     GFHLSNAELAARGLADRKGEPETFDARAFVARVRELGSGEPVSWPAYSRVLHEPVADA
                     VSVEPSARVVLVEGNYLLLDDEPWRDLRALLDEIWFVDADLGAIERRLMERHLAGGRS
                     QEEAERHLRESNLANVRLVLGVRDQADLCVTIDRADPDVRNLTDPATGKVIVPDA"
     gene            complement(7017..7334)
                     /locus_tag="VF752_04245"
     CDS             complement(7017..7334)
                     /locus_tag="VF752_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854789.1"
                     /translation="MATFAVIAEHPPQLCPTSNAVTKQMLKEGAGQIPQLAEQLGINI
                     VTLRVFGPDHILLGVLDADNIDAVRDFVFQSRLIQWNTTRIHATYSMEEAVAMVDEVE
                     TIF"
     gene            7422..10256
                     /locus_tag="VF752_04250"
     CDS             7422..10256
                     /locus_tag="VF752_04250"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX8854790.1"
                     /translation="MAEGNSFGARDTLSVGGSEYEIYRLDALQQKYDVARLPYSLKIL
                     LENLLRTEDGMAVTAEDVENLARWVASDEPSKEIAFSPSRVLMQDFTGVPAIVDLTAM
                     RDAMAELGGDPERINPLAPAELVIDHSIQVDEFLSRMSFARNAELEFERNRERYAFLR
                     WGQTAFDNFSVVPPNTGICHQVNLEYLARVVYTRDGVAFPDTLVGTDSHTTMINGLGV
                     LGWGVGGIEAEAAMLGQPVSMLIPQVIGFKLTGELAEGATATDLVLTVTQMLREKGVV
                     GKFVEYYGSGLANLPLADRATIGNMSPEYGATCGIFPVDAETLRYLEFSGRPKELVDL
                     VEAYTKEQGLFHDESSEEATYSDTLELDLSTVEPSIAGPKRPQDRVPLRVAKPEFEEA
                     LADLIGPVDDGDGTLNGDTPDEAVAETFPASDPPAFMAGTAPHPDNAPPHAPQEGAGA
                     AVADPPSTSVTATLDDGTEVDLDHGKVVIAAITSCTNTSNPSVMLGAGLLAKKAVERG
                     LTRKPWVKTSLAPGSKVVTEYLERAKLIEPLEELDFNLVGYGCTTCIGNSGPLPEAIS
                     NAIQENDLAAVAVLSGNRNFEGRIHPDVKMNYLASPPLVVAYALAGTMDIDLLNDPLG
                     DGSDGEPVYLRDIWPSGQEVAQAVESAVESDMFRKSYGEVFEGDENWNALEVPTGERY
                     AWDPESTYVKHPPYFEGMDAEPADELPQIGGARILALLADSVTTDHISPAGAIKPDSP
                     AGRYLIDHGVERKDFNSYGSRRGNHEVMMRGTFANVRLRNQLAPGTEGGFTVHLPDGE
                     QTTIFDAAMQYADEGVPLGVLAGREYGSGSSRDWAAKGPRLLGVRFVIAQSYERIHRS
                     NLVGMGVLPLQFPAGESVESLGLTGRERFDLEPLAEGARTLKVTATPDGGDPIEFEAT
                     VRIDTPNEWQYYRHGGILHFVLRSLAARAS"
     gene            10253..10705
                     /locus_tag="VF752_04255"
     CDS             10253..10705
                     /locus_tag="VF752_04255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8854791.1"
                     /translation="MSLPEGRLDHHDLCFGCGIANLFGLQMEIVRAERGGGVVGRFFV
                     KQDHQGPPGYAHGGVIAAALDDAMAVLLHASGKLAVTRSIEVDFRAPVPVGVFVVVEA
                     EVERREEGRVWLRSIARTEGEGATVVAEATAQFKELGDLPPEALSRAT"
     gene            10795..11187
                     /locus_tag="VF752_04260"
     CDS             10795..11187
                     /locus_tag="VF752_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX8854792.1"
                     /translation="MNVDVLKSVPFFKGLGRRDFKELAGHTDELDVRAGTVLCREGDF
                     GHEFFVIESGEFEVAKADGRTATLGPNDFFGEIALLEADRRNATVTAKTDGTVIVMNR
                     SDFRHLEHDHPEISARVREAIEQRRVPA"
     gene            complement(11184..11549)
                     /locus_tag="VF752_04265"
     CDS             complement(11184..11549)
                     /locus_tag="VF752_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854793.1"
                     /translation="MPELIDSPTRFEAGGEPPKAIEEFVGRVNSGEARVSVARMRSPA
                     GWHEPGQRPEFDEYSLVLDGLLRVESEAGTLEVRAGQAVLVRSGEWVRYSTPDGADYV
                     AVCLPAFSPETVHRDSSSA"
     gene            11647..12156
                     /locus_tag="VF752_04270"
     CDS             11647..12156
                     /locus_tag="VF752_04270"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8854794.1"
                     /translation="MQRARAAIGSFVFLLIAPGVAAGLIPWLITDWEADDWSLPIRIA
                     GALLIAAGAAVLLSAFARFVIEGLGTPAPVAPPQQLVIGGLYRYVRNPMYLAVEATIV
                     GQALFLGQPSLLLYALAFAAIVAAFVYGYEQPTLERQFGDQYEEYKKRVPAWIPRRPS
                     DSRKTGVGS"
     gene            12162..14150
                     /locus_tag="VF752_04275"
     CDS             12162..14150
                     /locus_tag="VF752_04275"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX8854795.1"
                     /translation="MAENLTRQILKEHLVDGELTPGSPIALRVDQTLLQDATGTMACM
                     QFEQLDVGRVQVKRAVQYVDHNVIQLDFKNPDDHRMLQALARKYGLDYSRPGNGICHY
                     VHIERYARPGEILVGADSHTTTSGALGMIAIGAGGLDVAVAMGGYPYEIACPEIVQVH
                     LTGKLNRPWVQAKDIILELLRRLSASGGKNKIFEFTGEGTADLIVPERGTIANMIAEL
                     GATSAVFPPDDQTRDWLKKQQRPEDFSEIGADEGAEYDDRIEIDLNELGPLVAKPQNP
                     DNVVPIEEVAGTELAQVCIGSSVNSGYADLALPGAILAHQGGQVVHESITATATPGSR
                     QILTAIAESGVYRQLVEGGVRMLEPVCGPCVGMGQAPPSNANSLRTFNRNFPGRSGTP
                     EDSVFLCSPAVAAVSMLTGKIEDPRQYGGAPEILPAPELKPYVDDRHIFKPADADEAE
                     RIEIPRGPNIKTPPEHTPLPDSIEARIATVQPDNISTGDLAPDGVIVMSYRSNIPAIA
                     EFTFQHRDPEFRKRMQEWGSGFIVGGENYGQGSSREHAALAPLQLGVRAVFAKSFARI
                     HRRNLVAQGILALTFKDDADYDRAEVGQCWELPNIKQELEDGAEEIIVRIKDSGDEFT
                     VSHDFAPKEREILIEGGLLHYLKERGEQVTGEQPVAGA"
     gene            complement(14151..14960)
                     /locus_tag="VF752_04280"
     CDS             complement(14151..14960)
                     /locus_tag="VF752_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8854796.1"
                     /translation="MQRSELRAMIELDDRHWWYRGRRRVLSSVLDRLELPPAAQILDA
                     GCGSGRTLDELQRYGSVTGIDASRRALAAARARGHSRVFAARVERLPFEDASFDLITC
                     LDVIEHTPDDVASLRELRRVARPGARMLVTVPAYQWLWSRHDDANLHYRRYRRGTLRD
                     SAEVAGWSVERTTYFDSLLLPPAAVVRLAQRLLPSPRGARSDLRLTPRALDPALALPM
                     YAEAVVLRLGVKLPAGLSLLAELVNAQPAGLRAIAWPLSEPAREVARQMFA"
     gene            15089..15811
                     /locus_tag="VF752_04285"
     CDS             15089..15811
                     /locus_tag="VF752_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8854797.1"
                     /translation="MTPAFLPGLTIVLPCFNEEDNVREAVAAALRAGRRFAEHCEVLV
                     VDDGSTDATGEIGVELVELHPEVRLLLHPVNLGYGAALRTGIRAATEPFVLLTDADLQ
                     FDLGELADFVPLTEDAEVIAGYRIDRQDSFSRRLAAAGWNALVRSLYGLRVRDVDCAF
                     KLIRRDLIQSFTLRSTGAMISTELLVKCIDAGGRVAEVGVHHYPRPAGRASGGDPRVV
                     AHAFRELAQLHSALTPVAKHGA"
     gene            15813..17612
                     /locus_tag="VF752_04290"
     CDS             15813..17612
                     /locus_tag="VF752_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8854798.1"
                     /translation="MGSVALSARRRVAALPAAVPSAWLAAGGIAGVAAALRLPGIGGM
                     RENFFYDAGVRSMSLSWHNFFVGAFDPSASLAVDKPPIDLWLQVASVKLLGFNRVALS
                     LPQALAGSVAVLIVYDLGRRLFGTAAGLCAAAALAVLPVSVVTSRSDTMDSLMMALAL
                     LAAWLVVRAVETGRARWLYLAALVLGIDFNVKLLEALVPLPAIALLYLIAAREPVRAR
                     AARLGAGLVVLVATSLSWAAAVSTLAVRNRPYPIGSTNGSVWNVLFVYNGLDRIRPPA
                     GTRVHAAAPPGLSRLFSSIFSGYIGVELLPALLAGVLALLAAAVVFWARREGTRLSMG
                     GAAMVAVWLLTGFLLFSRMGAVRLRYLEAMTPAVALALGAGTAALARQRPALAAPTVG
                     AIALVGAVYAFHVPVAGTVVALAVIAALVAVVLMLLRRPSAAVVGAMVAVLAAPTAFA
                     AKLAADHSSDAPAGGGGLTARWAAKIDRYARAHRHGARYLLAAAAPGKAAALIARDPQ
                     PILFLTSYRGRQFTSARELARLVRRGEVSTVVMDHRCSPSTPAGCAPAVNWARRHGID
                     VSKRAHLRKRGQIYSVGPRISARRARVQIHMNR"
     gene            17640..18341
                     /locus_tag="VF752_04295"
     CDS             17640..18341
                     /locus_tag="VF752_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8854799.1"
                     /translation="MNQAGSLDILVVDDESPLRETLRQSFTREGHRVMAVADGRAAID
                     RATTQGFDIVLLDVALGQGPDGYEVCRTLRARRNVVPIIMLTALDSEADAVLGLEAGA
                     DDYVTKPFGLAELRSRIRAVLRRAGGRGLGDEILSAGRVTLDRRRREVAADGQPVQLT
                     FSEFELLDTMMGEPGRLFNRQELLRSIWGESAYRDPRAIDVHVRHLREKLEEDPQTPR
                     LIVTVRGAGYRLQAP"
     gene            18338..19756
                     /locus_tag="VF752_04300"
     CDS             18338..19756
                     /locus_tag="VF752_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8854800.1"
                     /translation="MRPVRLIPHGLRARIVLAMILTAVVTLAVAALALLPPLEHRLKR
                     EELQALADTAVAARPGFLSLEEGQPTAGSRRLARLSRALARRTGGRVVVFDSHGQSLT
                     NTDPAPDSFEDVGQAAVRGKTVKSVASNGSGSDVARVAMPLRVRGHRYVIVLRKTLDD
                     APLAARVVKHAFLVASLAGLAIAVLLGVAITGRVLRRVRRLRDATSLVARRGPAVELA
                     PDHARDEVGDLARSFATMQTRLREQEQARRTFVATASHELRTPLASLRMMLELLEEDL
                     AREPADVEDAREQAASAGAQARRLASLAADLLDLSRIDSEVQLRSEPVALAEVGRAVT
                     AEFAGMAEFEADAEPCWASGDPGAVARIARILVDNAIRFSPPSHPVAATVKEEHGLAV
                     LRVQDSGPGVPERERDLIFNRFQRGSATGDQAGFGLGLAIGRELAERMGGTLRLEPSA
                     EGGARFALRLGLAVPTREPVPG"
     gene            19813..20694
                     /locus_tag="VF752_04305"
     CDS             19813..20694
                     /locus_tag="VF752_04305"
                     /inference="COORDINATES: protein motif:HMM:NF017468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8854801.1"
                     /translation="MSASVLGLFVAVAASVALNVSYLIQRVGSAGAPAISPLRPVATA
                     RGLLGSPVWVGGFALGSAGTAMQVVALVLAPISLVQGFIAGGLALAVPLARRVLGEEL
                     RPAEVAAVGIMVAGLACISLAGTGGHEPLRTEPAATYVLISSGIGLALVLVPGLRRTG
                     ERLGLAGGIFYGAAMVAVKALAHAGHGSVPAVLASPWLEVAVVLGAAGFFAFQRGLQV
                     GPAIPVAALMMAGTNVVAVLAGFLVLGDPLGATPLLAVARIAGFVAVGAATWMLAPAQ
                     VRVTRPSLVATAATRER"
     gene            20691..22142
                     /locus_tag="VF752_04310"
     CDS             20691..22142
                     /locus_tag="VF752_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854802.1"
                     /translation="MNTTTRLGLVRQDAALRIARPELRAILTSVGVLGLAAYGVLCWR
                     IVEASFVQPRLLVPSAHRHFPLWMRGVLRANGSPLPFHDFLNVLVALTMLYVVIVVCA
                     RAIPALIGLGAVAALTVLFTLAPPLLSTDVFNYIAYSRAAQLHGLNPYVHGPAALPND
                     PVYPFVGHLWNHVPSAYGPLFTLLTYELEPLGIHGELWALKAIAGAACIGCVALVWLC
                     AKRLEVPAVPAALFFGLNPLVLVWGVGGAHNDMVMTFALLLGLYLLLSRRPALGGAAL
                     TVATAVKLTAGLALPFAIVGVRPRWRVAAGALMAAGVGLAITYAEFGSRIRGMLDALR
                     FQGSLHFVGANVPGYVGYLLGHPTAGYVHPHILTELVVVGSALLLLRTVLQRSWLEST
                     GWALLLLLVTASWILPWYVVWALPMAALARRPWLRAGAVALTGVLVAMQISHLALIHD
                     HHHRHRHHHHRHHHALVHHHTAHGPRFAVRTGG"
     gene            22153..22305
                     /locus_tag="VF752_04315"
     CDS             22153..22305
                     /locus_tag="VF752_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854803.1"
                     /translation="MSEHQPRTEEEAEETVEQREAEPEETGDKSGEDEPGVNPEPDQG
                     YGGQFE"
     gene            complement(22327..22638)
                     /locus_tag="VF752_04320"
     CDS             complement(22327..22638)
                     /locus_tag="VF752_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX8854804.1"
                     /translation="MATADLTVLALGRPEVSASEYIAEIQRRLASQDKVRYRMHAMGT
                     SLEGSTRDILDVVAELHSVPFEQGIPRVYTVLKLDERRDKPDQTLDDKVRSVEERLAA
                     D"
     gene            complement(22640..>24064)
                     /locus_tag="VF752_04325"
     CDS             complement(22640..>24064)
                     /locus_tag="VF752_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8854805.1"
                     /translation="RLLIGGDWVDAADGRTFETIDPATGQPICAVAEAGAEDVDRAVS
                     AARGAFEGPWRSMPAAGRSLLINKLADLIEKNAEELAQLESLDNGKPVTIAGAVDVPF
                     TVAHLRYYAGWPTKMEGETIPVSWPNMFVYTLKEPVGVAGQIIPWNFPLLMTGWKLGP
                     ALASGCTVVLKPAEQTPLTALRVGQLIQEAGFPEGVVNIVTGGGETGAAIVDHPGVDK
                     IAFTGSTVVGRQIGEKCGRSLKRVTLELGGKSPNIILPDADLDAAIKGAFQGIYFNTG
                     QACQAGSRLFVPREHYDAVTGALAEAAKGAKVGPGLDPSTQFGPVVSAEQYERVIGYI
                     ESGKEQGAEVAAGGNGGDGGNGYFINPTLFTGVEDDMRIAREEIFGPVLVAMPYDDLE
                     EVARRANDTEYGLAAGVWTRDVGNAHRLAQLLQAGSVYINVWGPSDAAAPFGGVKASG
                     IGREMGHANLDAYLEIKTVWTNLA"
BASE COUNT         3534 a         8392 c         8520 g         3618 t
ORIGIN      
        1 acccggccgc gccacggcct gacgagcgcc gaagcaagcg cgtccggctt ccagccacgc
       61 cggcgcgcca ggtcgccgag cgtccggtgc tgatcgtcgc gggcccagcg aagcacggtc
      121 gcccggtgca cgcccagcag cgagtacaga cgtgtctcgt cgtagggcag ccagtggaac
      181 atcacccagt gctcgcaggg cagccagcgg ggactcgcgc ccggcggtgc gtcggaatcg
      241 cgggcgaccg cgggcccacc cgcgagcgcc agcaacgcga tcagcgcggc tgcgaaaacg
      301 aggatcctct tcatgcccct gttagtcgac attttcgacg caggtcgcta cctccctgcg
      361 gctataaatg gccccatggc ctcgctctgg cgtaactgga ccggcgacca gcgctgcgca
      421 ccggccgaga tcgccgagcc gggcagcgag gcggagctcg ccgaggtgat cgcgcgcgcc
      481 cgcgcgagag gactgccggt ccgcgcggtg ggcagcggtc actcgttcac ggacatcgcc
      541 tgcaccgacg gcgtgatggt gcggctcagg cgcatgaacc gtcttgtcca cgccgatccg
      601 gcgaccggtc gcgtccgcgt gcaggccggg atcacgctgc gcgagctggg ccagcgcctg
      661 gccgagcacg gcctggcgct cgagaacatg ggcgacatcg atgcgcagac gctcgcgggc
      721 gcgctagcga ccgccaccca cggcaccggc gcgcgctacg gcaacctcgc cacgcaggtg
      781 gcatcgctgc gtctgatgac cgccgacggt ttggtcgccg agctcagcga ggacgacgac
      841 gatcgcgaaa cgtggctcgc agcgcgcgtg tcgctcggcg cgctcgggat cgtgaccgag
      901 gtcgagctgc gctgcgtccc cgcgttcgcg cttcaccgca tcgacgagcc gcgtccgctc
      961 gccgagacgc tcgagcggtt ccactcgctc gtcgaggaga acgagcactt cgagttcttc
     1021 gcgatgccgt acaccgacgt cgcaatgtgc aggaagacga atcgcacgga acagccgccg
     1081 cagccgcgcg aacgcgtcgc cgcgtacgtg caggacgtcc tgctcgagaa tcgcgcgctc
     1141 gacctcttct gccgcgcggg ccgtgccgta cccagggcca tcccggcgct taaccggcgg
     1201 ctagcgggct tcttcagccg caccgagctc gtcgatcgga gccaccgggt gcatccgaac
     1261 cgccgagacg tgcgcttcac ggagatggag tacgcggttc cgcgcgaggc gggacccgac
     1321 gccgtgcggc ggatcttcga cctgatcgag cggcgccgcc tgaccgtgaa cttcccgatc
     1381 gaggtgcgcg ccgtggccgc ggacgacgct ttcctcagcc cggcgcacga tcgcccgacg
     1441 acctacatcg ccgtgcacag ctaccagggg atggagttcg agtcgtactt ccgcggcgtc
     1501 gaggcgatca tggacgagca cgagggccgc ccgcactggg gcaagcgcca ttaccagagc
     1561 gcccccacgc ttcgcgagcg ctatccgcgc tgggacagct tccaagacgt gcgggcccgc
     1621 ttggaccccg acggcgtgtt cgcgaacgac tacacgcggc gggtgctcgg cccggtcggc
     1681 gccgagctgc gcgcgccggc ctagccgcga acgaccgcta cgagctcggt cacgacaaga
     1741 ggatcgaact gggtgcccgc gcccgcctcg atctcggcga gcgccgagtc cctccggagc
     1801 gcgctgcggt acgggcggtt gctcgtcatc gccttgtagg cgtcgcaggc ggagatgatc
     1861 cggctcgcga gcgggatgcg gtcgcccttc agcccgtcgg ggtagccggt gccgtcccag
     1921 cgctcgtggt gaaagcgcac gattgtcgcc acgacctcga ggcccgggat gcggacgagc
     1981 gtctccgcgc cccacacggg gtggcagcgc atcatttccc actcgccgtc gtcgaggtgg
     2041 ccgcgtttgc gaaggatcgc gtcgggcacc gcgatcttgc cgacgtcgtg tagccgggcc
     2101 gcgaactcga gctccacgag cgcggcgcgg tcgagccgga gccgctcgcc gacctcgagg
     2161 gccaaatcca ccacgtcgtc cgaatgctgc gccgtgtacc cgtcgcgcat gtccatcgcc
     2221 gcggcgagcg cgtctacacg tgccgccacc gtcgcgtccg ggcgctcacg cgcctccgcg
     2281 tgcgcgagcg ccgcgctcgc gagctcggcg agctcggaca gctcctcgag ctcgcgctgg
     2341 ccgaagcgac ggcccggccg catgctcgtc acgcaaaggg cggcgcgcac gctgccctcc
     2401 cagctcaccg gcgcgcacgc gctcacgccg agtccatcgt gctggatcac aaacggggcg
     2461 gtctggcgcg acccgaagcc tgcgagcacc gagccggcca cgccctcgtc ggtgcccact
     2521 cgctccgcga tcgtgtgctc gtcgacgccg tgcgcggcaa ccgccatcgc cgcgcgcggg
     2581 tcgaggcggt cgcggatcac tatccaggac tgctcgcagt cgaggattcg gcaggtgtgg
     2641 cgtgcgagcc ggtcgagcac gagcgccggg tagccggtct cgtggatcgc cgtccgggct
     2701 ccgccttggc gctccgtcat ccgcggcgaa tgttcccgcc tggagttacg tacgtgcctg
     2761 ggataaacgc atgttcgacc aatgcagagg tgtgcctgca taaatcgggc agtaactaca
     2821 ggtaacagcg cccctcgccc cggtaagtgg ctacctcgcc caccagctcc gcgcccgata
     2881 cgagatacag gcggtcgaag cgctcgcaca gctcgccggc cttcgcgtga cggaaataca
     2941 cgcgatcgcc cacacgcagg cgctcggccg cggcgccaga cacgggcgtc tgcacctcgc
     3001 cggcgccctc catggggtcg agcctcagcc cgggcgggag gtgagggctc gggaggcggt
     3061 cgcgcccagg tgcgccgctc gcgatgtagc cgccgccgag caccgtgacg gtccggcgcg
     3121 atggccggcg cacgaccggc agcgcgaaca tcgcggccgg ccggaggctg aaggacgagt
     3181 atgcgtcgaa gagggtgggg gcgtagagcc cggaccccgc ggtcacctcg gtcaccgccg
     3241 gctcggcggc cgttcggtcg atgctgcccg tgcccccgcc gttcacgaac tcgagcagcg
     3301 cgacggcgtt cacggcctcg acgaccgagg cccgccgctc ggcgatctcg cgagcggacg
     3361 cctcctggat gcgccggatc acggcgccgc ggacccgccg cccggcgggc gcgtcgccca
     3421 cgcccgcgat gtggccctcg tagcccatga gccccacgag ctccaggcgc gggcggcgcg
     3481 cgatctcgcg cgcgagcagc tgggcctgct gcggagtgcg gatcggcgag cgcttcgggc
     3541 cgaccttcag gcgcccgccg agcgcccacc acgacaggtc gatctcgatg cacacgcgca
     3601 cgggccgcgc ggggtcgccc gcggcggcct cgatgaagtc gaggtgctcg agcgagtcga
     3661 ccatcagcgc cggcgggcgc tcgccgtcga tgcgcgtgag ctgcgcgaga gcctcccggt
     3721 cggccgtcgg ataggcgagc aggatgtcgt cgaagcccag ctcgttcagc cagagcgact
     3781 ccgccagcgt gaacgtcatg aggccacgga agccgacgtc gcgatcgagc acatcgcgca
     3841 gcaggcgcct gcagcgcacc gacttgctcg ccaggcgcag cggcttgcca cgtgcgcgcg
     3901 cggccatggc ggtcgcgttc tcccacatgg catcgaggtc cacgaacgcg aacggcgcgt
     3961 cgatgccctc gaaggcacgc ccgtagcggt cgagctcgct caccgggcgc gcaggtagac
     4021 ggggttcgtg agcacccacg tgacctcgcg acccttgagg tcgagccggg cgtccacgcg
     4081 gtaggcgccc ggctcggacg cctcgaagtc gatcgctcgg ccggcattgc gggcgagctc
     4141 gtcgccgttg cgcaacagac gcagcgtcgc gtgccgcggc gagcgcgcat gcaactcgta
     4201 gccctccccg agctcggcct cttcgcccat ccgcagcgtg ccagcctcgc cttcggccca
     4261 gaaggagaag ccgtgcgcgg gagcggcgcc gtcgaacgcc atgtagcagc ggccctcgcg
     4321 cagcgccgag tacacctgcg tacggtcgtg gtcgaggtcg cggctgagcg gctcctcgca
     4381 cagcgcgtgc gtgcgcagca tcgagaacga gcgccggtag ctcatcagcc gcagcgggac
     4441 gcggccgcgc atccgcaggc cgacttggtg ggcgtcggtg cctgcaatcg cgaccatccg
     4501 gcgccgcgcg cacaccgcgt cccaggccgc gacgttgccg gccggcgggt gatccaccac
     4561 gcgccggggg gccgcgatga agcgcgccac cgcgggcagg ctcgcgagcc gctcggccgt
     4621 gtcgttcagg aagctccaca gctcgagccc gtccacgcgg tcgtcggtga cctcggcgaa
     4681 cggcatcggc tcgacgcggc tgaagcgctc agaaccgtgg gagaaggggt gagccgcgaa
     4741 gccgaagccg ccggcctcgc gtaccgcggc ggcaatctcg gggccgctca ggcggcggct
     4801 cacgaggcgg ttgataccga acgcgaggaa gtggtcgcgg ttcggcggag agatctcggt
     4861 gcccaccagg agaagcaccg gaccgtgcca gccctcctcg ccgttatcgc gcgccgagag
     4921 cgtgtcgtgg tcggtgagca ggaccacgtc ggcgcccgct cgagcggcgg cgtcggcgat
     4981 ctccggcacc gtccccgtcc cgtccgaata gatcgaatgc acgtgcacta cgcacacgag
     5041 ctcgtgcagc tccggcgcgc tcacgaggcg agcgtacagg tgaagatctc ccagacagcg
     5101 cgaagggcgg caagagcgcc aacgcgaagc gcaagctcaa gctgcagctg gcgcgccggt
     5161 aggcttcggg ccgcgggagg cccggagccg tcaggaggat cagccatggc agtgatcgag
     5221 agcccagaga agatcggaat tcgcccgttc cacgagttcg acgacgcaaa cgacacctgg
     5281 cctcgcggtg agcggccgga tgcgatccgc gacgccgcgc gcgagttccg cggccgcttc
     5341 aaggcccagg gagagatcca cgcggtgcgc acgatcgacc tggtctctgc cggctatccg
     5401 accgcgttcg cgctcgccgg cgcggcgaag ggcctcaatc cgtacatcaa catccgcaac
     5461 cgactcgtgg tcgtccagtt cgacgacttc gacgggcagc gcaggacgct cgtgtgggag
     5521 ccgaccgtgc cggagggctc ggtcgaggca cccttctacg cgcagctgct cgagcgctac
     5581 ggcgagttcc tctcggagaa ggtgttcgcg aagtacttca acaccgtcgg cgaggcgctc
     5641 gcgctgtgcg ggctgcgccc ggaggacgtg gactacgtgt ccttcgacca cctgcacgtg
     5701 caggacatgc gcatcctgat gggcacgacc gagccggtcg acggcgagcc ggtgccgcgg
     5761 ccgccgttct tcccgaacgc gaagttcctc tgccagcgca aggaggttga cacgctccac
     5821 tcgatgcacc cgatgcagtg ggcctggtac gtgccgcacg ggatggacaa ggtgatcgac
     5881 gacaacctcg tcctggtcga cggggacgtg gagctcggcg tcggcgttgc gctcgtctgg
     5941 acgccggggc acaccgacgg caaccagtcg ctctgcatca acacgccgga cggcatctgg
     6001 atcacgtcgg agaacggcgt gtgcgcggac tcgtggcacc cgcggctctc gaagattccg
     6061 ggcgtgagga agtacgccga gttctacaac cgcgaggtcg tgctgaactc gaacacgctc
     6121 gaggactcga tcgaccagta cgactcgatg atcaaggaga aggcgctcgc cgacccgaac
     6181 cggcgcgacc cgcgcttcct caatgtcttc ccgtcgtccg agatggagag cttcaggcgg
     6241 cagtggccgg tcgtgcccac gttcgtgtac ggaggcatga actacggcga gatccagcga
     6301 gccgcatagc gtcgcggcgc tggccgaccg cgctgggcgc ctgcagagcg gcgggcgtcg
     6361 cgcgctgatc ggcatcacgg gcccgcccgc ggccggcaag acgacgctga tggaagcgct
     6421 ggcgcggcga ctccgcgccg acggctatgc cgccgcgggc gtgccgatgg acggttttca
     6481 cctctcgaac gccgagctcg ccgcgcgcgg gttggccgac cgcaagggcg agccggagac
     6541 cttcgacgca cgcgcgttcg tggcgagggt gcgagagctc gggagtggag agcccgtgag
     6601 ctggcccgcg tacagccgcg tgcttcacga gccggtcgcg gacgccgtca gcgtcgagcc
     6661 gtcggcgcgc gtggtactgg tggaggggaa ctacctgctg ctcgacgatg agccctggcg
     6721 cgacttgcgc gcgctgctcg acgagatctg gttcgtggac gccgacctcg gcgcgattga
     6781 gcgacggctc atggagcgac acctcgccgg cggccgctct caggaggaag ctgagcgcca
     6841 cctgcgcgag tccaacctcg cgaacgtgcg cctcgtgctc ggcgtgcgcg accaggcaga
     6901 cctttgtgtg accatcgatc gtgctgaccc ggacgtgcgg aacctgaccg accccgccac
     6961 cggcaaggtg atcgtgcccg acgcctgatt tcccggcgtg ttgtgttcgt cttcggctag
     7021 aagatcgtct cgacctcgtc caccatcgcg acggcctcct ccatcgagta cgtggcgtgg
     7081 atcctcgtgg tgttccactg gatgaggcgg ctctggaaga cgaagtccct gacggcgtcg
     7141 atgttgtccg cgtcgaggac cccaaggagg atgtggtccg gcccgaagac gcgcagggtg
     7201 acaatgttga tgcccagctg ctcggcaagc tgcggtatct ggccggcgcc ctccttcagc
     7261 atctgcttcg tgacggcgtt ggaggtcggg cagagctggg gcggatgctc cgcgattacg
     7321 gcgaacgttg ccatgaggaa cctccagggt ttgccggacc tcaccctaac tctctgaccc
     7381 gtggcctgct agcggcagcc gacattcact agattccaag catggccgag gggaacagct
     7441 tcggggcgcg cgacacgctg agcgtgggcg gcagcgagta cgagatctac cggctcgacg
     7501 cgctgcagca gaagtacgac gtcgcccggc tgccctactc gctgaagatc ctgctcgaga
     7561 acctgttgcg cactgaggac ggcatggcgg tgacggccga ggacgtcgag aacctcgccc
     7621 gctgggtggc gagcgacgag ccgagcaagg agatcgcgtt cagcccgagc cgcgtgctga
     7681 tgcaggactt caccggagtg ccggcgatcg tggatctgac cgcgatgcgc gacgcgatgg
     7741 cggagctcgg gggcgacccg gagaggatca acccgctcgc gccagcggag ctcgtgatcg
     7801 atcactcgat ccaggtcgac gagttcctct cgcgcatgtc gttcgcgcgc aacgcggagc
     7861 tcgagttcga gcgaaaccgc gagcgctacg cgttcctgcg ctggggccag accgcgttcg
     7921 acaacttcag cgtggtgccg ccgaacaccg ggatctgcca ccaggtgaat ctcgagtacc
     7981 tcgcccgcgt cgtgtacacg cgcgacggcg tggcgttccc cgacacgctg gtcggcaccg
     8041 actcccacac gacgatgatc aacggccttg gcgtgcttgg ctggggagtg ggcggcatcg
     8101 aggccgaggc cgcgatgctc ggtcagccgg tgtcgatgct gatcccgcag gtgatcggct
     8161 tcaagctcac cggtgagctc gccgagggag ccaccgcgac ggacctggtc ctgaccgtca
     8221 cgcagatgct gcgcgagaag ggcgtggtcg ggaagttcgt cgagtactac ggctcgggtc
     8281 tcgcgaacct gccgctcgcg gaccgcgcga cgatcggcaa catgtcgccc gagtacggcg
     8341 cgacctgcgg catcttcccg gtcgacgccg agacgctccg ctacctcgag ttcagcggcc
     8401 gccccaagga gctcgtggat ctcgtcgagg cctacaccaa ggagcagggc ctcttccacg
     8461 acgagagctc tgaggaggcg acctactcgg acacgctcga gctcgatctc tcgacggtcg
     8521 agccgtcgat cgcagggccg aagcgcccgc aggaccgtgt gccgttgcgc gtcgcgaagc
     8581 ctgagttcga ggaggcgctc gcggacttga tcgggccggt cgacgacggc gacggcacgc
     8641 tcaatggcga cacgccggac gaggccgtgg ccgagacgtt cccggcctca gatccgcccg
     8701 cgttcatggc cgggacagcg cctcatccgg acaacgcgcc gccgcacgcg ccccaagagg
     8761 gcgccggcgc cgcggtggct gacccgccgt cgacgagcgt gaccgcgacc ctcgacgacg
     8821 gcactgaggt ggacctcgac cacggcaagg ttgtgatcgc cgcgatcacg agctgcacga
     8881 acacctcgaa cccgtcggtg atgctgggcg ccggcctgct tgccaagaag gcggtggagc
     8941 gcggcctcac tcgcaagccg tgggtgaaga cctcgctcgc gcccggttcg aaggtcgtga
     9001 ccgagtatct cgagcgggcg aagctgatcg agccgctcga ggagctcgac ttcaacctgg
     9061 tcggctacgg ctgcaccacc tgcatcggca actcgggacc gctgcccgag gcgatctcga
     9121 acgcgatcca ggaaaacgac ctcgccgccg tcgccgtgct gtcggggaac cgcaacttcg
     9181 aggggcgcat ccaccctgac gtgaagatga actacctcgc gtcgccgccg ctggtcgtgg
     9241 cctacgcgct cgccggcacg atggacatcg atctcctgaa cgatccgctc ggcgacggct
     9301 ccgacggcga gccggtctac ctccgcgaca tctggccctc cggccaggag gtggcgcagg
     9361 cggtggagag cgcagtcgaa tcggacatgt tccgcaagag ctacggcgag gtgttcgagg
     9421 gcgacgagaa ctggaacgcg ctcgaggtgc cgaccggcga gcgttacgcc tgggatccgg
     9481 agtcgacgta cgtgaagcac ccgccgtact tcgagggcat ggacgccgag ccggcggacg
     9541 agctgccgca gatcggcggc gcgcgcatcc tcgcgctgct ggccgactcc gtgaccacgg
     9601 accacatctc accggcgggc gcgatcaagc ctgattcgcc cgcgggcagg tacctcatcg
     9661 accacggggt cgagcggaag gacttcaact cgtacgggtc gcggcgcggc aaccacgagg
     9721 tgatgatgcg cggcacgttc gcgaacgtgc ggctgcgcaa ccagctcgct cccggcaccg
     9781 agggcggctt caccgtgcac ctgccggacg gcgagcagac gacgatcttc gacgcggcga
     9841 tgcagtacgc cgacgagggc gtgccactgg gcgtgctcgc cgggcgcgag tacgggtccg
     9901 gctcctcgcg cgactgggcc gcgaaggggc cgcgcctgct cggcgtgcgc ttcgtgatcg
     9961 cgcagagcta cgagcgcatc caccggtcga acctcgtggg catgggcgtg ctgccactgc
    10021 agttcccggc cggcgaatcg gtcgagtcgc tcgggctaac gggccgcgag cgcttcgacc
    10081 tggagccgct cgcggaggga gcgcgaacgc tgaaggtgac cgcgacgccc gacggcggcg
    10141 acccgatcga gtttgaggcc accgtccgca tcgacacacc gaacgagtgg cagtactacc
    10201 ggcacggcgg catcctgcat ttcgtgctgc gctcgctcgc cgcgcgcgcc tcttgagtct
    10261 cccggaggga cggctcgatc accacgacct ctgcttcggc tgcggaatcg caaatctgtt
    10321 cggcctccag atggagatcg ttcgggccga gcggggcggg ggagtcgtgg gtcgcttctt
    10381 cgtgaagcag gaccaccagg gcccgccggg ctacgcgcac gggggcgtga tcgccgccgc
    10441 gctcgacgac gcgatggccg tgctgctgca cgccagcggc aagctggccg taacgcgctc
    10501 gatcgaggtc gatttccgcg cgcccgtgcc ggtcggcgtg ttcgtggtgg tggaggccga
    10561 ggtggagcgc cgcgaggagg ggcgggtgtg gctccggtcg atcgcacgga ccgagggcga
    10621 gggcgcgacc gtggtcgccg aggcaaccgc ccagttcaag gagctgggcg acctgccgcc
    10681 ggaagcactt tcccgagcaa cctgagcggt cgccgcactg agccgggcgg tgcggttgcc
    10741 gcggtggtct tcgcgcgcat acgatcgctc taacccgaag gggaggagtg ttccgtgaac
    10801 gtcgatgtgc tgaagagcgt gccgttcttc aaggggcttg ggaggcggga tttcaaggaa
    10861 ctcgccggtc acaccgacga gctggacgtg cgcgccggaa cggtgctctg ccgcgagggc
    10921 gacttcggcc acgagttctt cgtgatcgag tcgggcgagt tcgaggtcgc gaaggccgac
    10981 ggccgcaccg cgacgctcgg gccgaacgac ttcttcggcg agatagcgct gctcgaggcc
    11041 gaccgccgca acgccactgt gaccgccaag accgacggga ccgtgatcgt gatgaaccgg
    11101 tccgatttcc ggcacctcga gcacgatcac cccgagatct cggcgcgggt ccgcgaggcg
    11161 atcgagcagc ggagggtgcc cgcctaagcg ctactcgagt cgcgatggac cgtctccggc
    11221 gagaacgccg gtaggcacac ggccacgtag tccgcgccgt ccggcgtgga gtaacgcacc
    11281 cattcaccgc tgcgcacgag tacggcctgc cccgctcgaa cttcgagcgt cccagcctcg
    11341 ctctccaccc gcagtagtcc gtcgagcacg agcgagtact cgtcgaactc gggccgctgg
    11401 ccaggctcgt gccagcccgc cggcgagcgc atgcgagcga cgctcacgcg cgcctcgccg
    11461 ctgttgacac ggccgacgaa ctcctcgatc gccttcggcg gctcgccgcc ggcctcgaag
    11521 cgggtgggcg agtcgatcag ctcgggcacg cccgcagcgt aaccggaggc agtgaggggg
    11581 gtgggctgtt ttgacattga tagtcatggt caaaacggcc caggtccgtt gagggaggca
    11641 cgttgaatgc agagagcgcg tgcggccatc ggcagcttcg tcttcctcct cattgccccc
    11701 ggcgtggcgg ccggtctcat cccttggctg atcaccgact gggaggccga cgactggtcg
    11761 ctgccgattc gcatcgccgg cgcgctcctc atcgcggctg gcgccgcggt gctactcagc
    11821 gcgttcgctc gcttcgtgat cgagggactc gggacgccgg ctccggtggc gcccccgcag
    11881 caactcgtga ttggaggcct ctaccgctac gtgcgcaacc cgatgtacct ggccgtcgag
    11941 gccacgatcg tcggccaggc cctcttcctc ggacagccct cattgctgct gtacgcgctt
    12001 gcgttcgccg cgatcgtcgc ggcgttcgtc tacggctatg agcagccgac cctcgagcgg
    12061 cagttcggcg accagtacga ggaatacaag aagcgtgttc ccgcctggat tccgcgccgc
    12121 ccgagtgaca gccggaaaac cggggtaggt tcttagccgc gatggccgag aacctcaccc
    12181 gccagatcct caaggagcac ctggtggacg gcgagctcac gccgggctcg ccgatcgcgc
    12241 tcagggtcga ccagacgttg ctccaggacg ccaccggaac gatggcgtgc atgcagttcg
    12301 agcagctgga cgtggggcga gtgcaggtga agcgggccgt gcagtacgtc gatcacaacg
    12361 tgatccagct cgacttcaag aacccggacg accaccggat gctgcaggcg ctggcgcgca
    12421 aatacgggct cgactactcg cggcccggca atggcatctg ccactacgtc cacatcgagc
    12481 gctacgcgcg accgggcgag atcctcgtgg gcgccgactc gcacaccacc acgagtggtg
    12541 cactcggcat gatcgcgatc ggcgccggcg gcctcgacgt ggcggtcgcg atgggtggct
    12601 acccctacga gatcgcctgc ccggagatcg tgcaggtgca cctcaccgga aagctgaacc
    12661 ggccctgggt ccaggcgaag gacatcatcc tcgagctgct gcgccggctg tcggcgagcg
    12721 gcggaaagaa caagatcttc gagttcaccg gcgaggggac ggcggatctg atcgtgcccg
    12781 agcgcggcac gatcgccaac atgatcgccg agctcggcgc cacctcggcc gtgttcccgc
    12841 ccgacgacca gacgcgcgac tggctcaaaa agcagcagcg ccccgaggac ttcagcgaaa
    12901 tcggggcgga cgaaggcgcc gagtacgacg accgcatcga gatcgacctg aacgagctcg
    12961 gcccgctggt cgcgaagccg cagaacccgg acaacgtggt gccgatcgag gaggtcgcgg
    13021 gcaccgagct ggcgcaggtg tgcatcggct cgtcggtgaa ctcgggctac gccgacctgg
    13081 cgctacccgg cgcgatcctc gctcaccagg gcggccaggt cgtgcacgag tcgatcacgg
    13141 ccaccgcgac gccgggctcg cggcagatcc tcaccgcgat cgcagagtcg ggcgtctacc
    13201 ggcagctggt ggagggaggc gtgcggatgc tcgagccggt gtgtggcccg tgcgtgggta
    13261 tggggcaggc gccgccgtcg aacgcgaact ccctgcgcac gttcaaccgc aacttccccg
    13321 gccgctcggg cacgccggag gacagcgtgt tcctctgctc gcccgcggtc gccgcagtgt
    13381 cgatgctcac gggcaagatc gaggacccgc gccagtacgg cggtgctccg gagatcctcc
    13441 cggcgcccga gctcaagccc tacgtggacg accgccacat cttcaagccg gccgacgcgg
    13501 acgaggccga gcgcatcgag atcccgcgcg ggccgaacat caagacgccg cccgagcaca
    13561 cgccactgcc cgactcgatc gaggcgcgca tcgcgactgt gcagcccgac aacatctcga
    13621 ccggcgacct cgcgccagac ggcgtgatcg tgatgagcta ccgctcgaac atccctgcga
    13681 tcgcggagtt cacgttccag caccgcgacc cggagttccg caagcgcatg caggagtggg
    13741 ggagcggctt catcgtcgga ggcgagaact acggccaggg ctcctcacgc gagcacgccg
    13801 cgctcgcgcc cctccagctc ggcgtaaggg ccgtgttcgc gaagtcgttc gcgcgcatcc
    13861 accgccgcaa cctggtggcg cagggcatcc tcgcgctcac gttcaaggac gacgccgact
    13921 acgaccgcgc ggaggtcggc cagtgctggg agctgccgaa catcaagcag gagctcgagg
    13981 acggagcgga ggagatcatc gtgcgcatca aggattcggg ggacgagttc acagtgagcc
    14041 acgacttcgc cccgaaggag cgcgagatcc tgatcgaagg tggcctcctg cactacctca
    14101 aggaacgcgg cgagcaggtg acaggcgagc agccggtcgc tggcgcctga tcaggcgaac
    14161 atctggcgcg ctacttctcg ggccggctcg gataggggcc aggcgatcgc acgtaggccc
    14221 gcgggctggg cgttcaccag ctccgccagc agcgacagtc cagccggcag cttgaccccg
    14281 agccgcagca cgaccgcctc cgcgtacatc gggagcgcga gcgccggatc gagagcgcgg
    14341 ggcgtgagcc gtaggtcgga acgcgcaccg cgtggcgacg gcagcagccg ctgggcaagc
    14401 ctcacgactg cggcgggcgg caggagaagg ctgtcgaagt aggtcgtccg ctcgacgctc
    14461 cagccggcga cctccgccga gtcgcgcagc gtgccgcgcc gatagcggcg gtagtgcagg
    14521 ttcgcgtcgt cgtggcgcga ccacagccac tggtaggcgg gcacggtcac gagcatgcgt
    14581 gcgcccggac gcgcgacccg gcgcagctcg cgcagcgagg cgacgtcgtc cggcgtgtgc
    14641 tcgatcacgt cgaggcacgt gatcaggtcg aagctcgcgt cctcgaacgg caggcgctcg
    14701 actcgtgccg cgaacacgcg cgagtggccc cgcgcacgcg ccgcggcgag cgcgcggcgg
    14761 ctcgcgtcga tgccggtgac cgacccatac cgctgcagct cgtcgagcgt acgaccggaa
    14821 ccgcagccgg cgtcgaggat ctgcgcggca ggcggcagct cgagccggtc gagcacgctc
    14881 gacagcaccc gtcgcctgcc gcggtaccac cagtggcgat cgtcgagctc gatcatcgcc
    14941 ctgagctccg atctctgcat cgcgcggatg ctgcccccgc gccggtgtgc gatggcggaa
    15001 attctgttgc gatcgcgtta gacggggtct aacccgagcg acccgctgcc ctaaccgtcc
    15061 ttaacgcgcc cggggtgacc ctgctggcat gacgcctgcc ttccttcccg gcctgaccat
    15121 cgtgcttccc tgtttcaacg aagaggacaa cgtgcgcgag gcggtggccg cggcgcttcg
    15181 tgcagggcgt cgcttcgccg agcactgcga ggtgctggtc gtggacgacg gcagcaccga
    15241 cgccacgggg gagatcggcg tcgagctcgt cgagctacat cccgaggtgc ggttgctgct
    15301 ccacccggtg aacctcgggt acggtgcggc gctccgcacc ggcatccgcg cggccacgga
    15361 gccgttcgtg ctgctcaccg acgccgacct ccagttcgac ctcggcgagc ttgcggactt
    15421 cgtgccgctc acggaggacg cggaagtgat cgcgggctac cggatcgacc ggcaggactc
    15481 gttcagccgt cgcctcgccg cagccggctg gaacgccctg gtgcgctcgc tgtacgggct
    15541 tcgtgtgcgc gacgtggact gcgcgttcaa gctgatccgg cgggatctga tccagagctt
    15601 cacgcttcgc tcgaccggcg cgatgatcag caccgagttg ctcgtgaagt gcatcgatgc
    15661 gggcgggcgg gtggcggagg tgggggtgca tcactaccct cgccccgcag gacgcgcgag
    15721 cgggggcgac ccgcgcgtgg tggcgcacgc cttccgtgag cttgcacagc tccacagcgc
    15781 gctcacgcca gtggccaagc acggagcgta ggttgggtag cgtcgcgctg agcgcccgcc
    15841 ggcgcgtggc cgcgctgccg gcagcggtgc cgagcgcttg gctcgccgcg ggcggcatcg
    15901 ccggcgtggc agccgcgctc cggctgccgg gcatcggcgg gatgcgcgaa aacttcttct
    15961 acgacgccgg cgtgcgcagc atgtcgctgt cgtggcacaa cttcttcgtc ggcgcgttcg
    16021 acccgagcgc atcgctcgcg gtcgacaagc cgccgatcga cctgtggctg caggtggcga
    16081 gcgtgaagct gctgggcttc aaccgggtgg cactgtcgct gccacaggca ctcgccgggt
    16141 cggtggcggt gctcatcgtc tacgacctcg ggcgccgcct gttcggcacc gccgccggcc
    16201 tgtgtgccgc cgcggcgctc gcggtgctgc cggtgtccgt ggtcacctcg cgcagcgaca
    16261 cgatggattc gttgatgatg gcgctcgccc tgctcgcggc gtggctcgtc gtgcgggcgg
    16321 tggagacggg ccgcgcgcgc tggctgtacc tggccgcgct cgtgctcggc atcgatttca
    16381 acgtgaagct gctcgaggcg ctggtgccgc tcccggcgat cgcgctgctc tacctgatcg
    16441 ccgcgcgtga gccggtgagg gcacgcgcgg cgcggctagg agccgggctg gtggtgctgg
    16501 tcgccacctc cctgtcgtgg gccgccgccg tctccacgct tgccgtgcgc aatcgcccct
    16561 acccgatcgg atctacgaac ggcagcgtct ggaacgtgct gttcgtctac aacggcctcg
    16621 accggatccg gccccccgcg gggacgcggg ttcacgccgc tgctccgccc ggcctcagcc
    16681 ggctcttcag ctcgatcttc tccggctaca tcggcgtgga gctgctgccc gcgttgctcg
    16741 ccggggtgct cgccttgctt gccgccgcgg ttgttttctg ggcgcgtcga gagggcaccc
    16801 gcctgagcat gggcggcgcc gcgatggtgg cggtgtggtt gctcaccggc ttcctgctct
    16861 tttcgcgcat gggcgccgtc aggctgcgct acctcgaggc gatgacgccc gctgtcgcgc
    16921 ttgcgctcgg cgcgggtaca gcggcgctcg cgcggcagcg cccggctctg gccgcgccca
    16981 ctgtcggcgc gatcgcgttg gtcggcgcgg tgtacgcgtt ccacgtacct gttgcgggca
    17041 ccgttgtggc cctcgcggtg atcgctgcgc tggtcgcggt ggtcctgatg ctcctgcgac
    17101 ggccgtccgc ggcagtcgtc ggcgcgatgg tggccgtgct cgccgcgccc acggccttcg
    17161 ccgcgaagct ggcggccgat cattcgtcgg acgcgccggc gggtgggggc gggctcaccg
    17221 cgcgctgggc agcgaagatc gaccgctacg cccgcgcgca tcgtcatggc gcgagatacc
    17281 tgctcgccgc cgcggcgccg gggaaggccg ccgcgctgat cgcgcgcgac cctcagccga
    17341 tcctcttcct cacgagctac cggggacggc agttcacgag cgcgcgcgag ctcgctcggc
    17401 tcgtgcggag gggcgaggtg agcacggtgg tgatggatca ccggtgctcg ccgagcacgc
    17461 cggcgggctg cgctccggcg gtgaactggg cgcgccgcca cggcatcgac gtgtcgaaac
    17521 gcgcgcactt gagaaagcgc ggccagatct actcagtggg accgcggatc agtgcgcgtc
    17581 gtgcacgcgt gcaaatccac atgaaccgtt gagattccag ggatagcttg acccgcggga
    17641 tgaaccaagc cggcagcctg gacatcctcg tggtcgacga cgagtctccg ctcagggaga
    17701 cgcttcgcca gtcgttcacg cgcgagggcc accgggtgat ggcggttgcg gacggccgcg
    17761 ccgcgatcga ccgagcgacc actcaaggct tcgacatcgt gctgctcgac gtggcgctcg
    17821 gccaggggcc ggatggctac gaggtgtgcc gcactctgcg cgcccgccgc aacgtggtgc
    17881 cgatcatcat gctcacggcg ctcgacagcg aggccgatgc cgtgctcggg ctcgaagcgg
    17941 gtgcggacga ctacgtgacg aagcccttcg gcctcgccga gcttcgaagc cggattcgcg
    18001 cggtgctacg gcgcgcagga ggccggggcc tcggcgacga gatcctctcg gcgggtcgcg
    18061 tcacgctcga caggcggcgc cgcgaggtcg ctgcggacgg ccagcctgta cagctcacgt
    18121 tctccgagtt cgagctgctc gacacgatga tgggcgagcc cggacggctc ttcaaccgcc
    18181 aggagctgct gcgctcgatc tggggcgaga gcgcctaccg agacccgcgc gcgatcgacg
    18241 tgcatgtgcg gcacctgcgc gagaagctcg aggaagaccc gcagacgccg cgcctgatcg
    18301 tgaccgtgcg cggcgcgggc tacaggctgc aggcgccatg aggccggtcc ggctgatccc
    18361 gcacgggctg cgcgcgcgga tcgtgctcgc gatgatcctc actgcggtcg tgacgctggc
    18421 ggtcgcggcg ctggcgctgc tgccgcctct cgagcaccgc ctgaagcgcg aggagctgca
    18481 ggcgctggcc gacaccgcgg tggccgcacg tccgggcttc ctctccctcg aggagggcca
    18541 gccgacggcc ggctccaggc ggctcgcgcg actgtcccgg gcgctcgcga ggcgcacggg
    18601 cgggcgcgtg gtcgtgttcg acagtcatgg ccagtcgctg accaacaccg acccggcgcc
    18661 ggacagcttc gaggacgtgg ggcaggcagc ggtacgcggc aagaccgtga agagcgtggc
    18721 gagcaacggc tcaggcagcg acgtcgcgcg cgtggcgatg ccactgcggg tgcgaggcca
    18781 tcgttacgtg atcgtgctgc gcaagacgct cgacgacgct ccgctcgccg cgcgcgtggt
    18841 caagcacgcc ttcctcgttg ccagcctggc gggcctcgcg atcgccgttc tgctcggcgt
    18901 cgccatcacc ggccgggtgc tgcgcagggt gcggcggctg cgcgacgcga cttctctcgt
    18961 ggcccggcgc ggccccgccg tcgagctcgc ccccgaccac gcacgcgacg aggtgggcga
    19021 cctggcccgc tcgttcgcga cgatgcagac gcgcctccgc gagcaggagc aggcgcggcg
    19081 caccttcgtg gccacggcgt cgcacgagct ccgcacgccg ctggcgtcgc tgcggatgat
    19141 gctcgagctg ctcgaggagg acctggcgcg ggagcccgcc gacgtggagg acgcccgcga
    19201 gcaggcggca agcgccgggg cgcaggctcg gcgcctcgcg tcgctcgcgg cggacctgct
    19261 cgatctcagc cggattgaca gcgaggtgca gctgcgcagc gaaccggtcg ccctcgcgga
    19321 agttggccgg gcggtgacgg ccgagtttgc gggcatggca gagttcgagg cggacgcaga
    19381 gccgtgctgg gcaagcggcg atcccggcgc ggtggcacgc atcgcgcgca tcctcgtgga
    19441 caacgccatt cgcttctcgc ctccgtcgca cccggttgcc gcgacggtca aggaagagca
    19501 cggcttggct gtgctgaggg tccaggactc cggccccggc gtccccgaac gcgagcggga
    19561 cctgatcttc aaccgcttcc agcgcggcag cgcgacgggc gaccaggcgg gcttcggtct
    19621 ggggctcgcg atcgggcgcg agctggccga gcgcatgggc gggacgctgc ggctggagcc
    19681 ctcggctgag ggaggcgccc gcttcgctct ccggctgggt ctcgcggtgc ccacgcgcga
    19741 gcccgtgccg ggctaacgcg atcccaacat cgaggacgcg attgctaacg gcgtcgcgga
    19801 cagattctcg gggtgagcgc atccgtgctg ggtttgttcg tcgcggtggc cgcctcggtc
    19861 gccctgaacg tgagctacct gatccagcgg gtggggtcgg cgggcgcgcc ggcgatatca
    19921 cccctgcggc ccgtcgcgac cgcccgcggc ctgctcggct cgccggtgtg ggtcggcggc
    19981 ttcgcgcttg ggagcgccgg gacggccatg caggtcgtgg cgctcgtcct ggccccgatc
    20041 tcgctcgtgc agggcttcat cgccggcggg ctcgcgctgg ccgtgccact cgctcggcgc
    20101 gtgctgggcg aggagttgcg cccggcggag gtcgccgccg tggggatcat ggtcgcgggg
    20161 cttgcgtgca tctcgctggc aggtaccggc gggcatgagc cgctccgcac cgagcccgcg
    20221 gccacctacg tcctgatctc cagcgggatc gggctggcgc tcgtgctggt gccgggcctg
    20281 cgccggaccg gcgagcggct gggcctcgca ggcgggatct tctacggcgc cgcaatggtg
    20341 gcggtgaagg cgctcgcgca cgcggggcac ggcagcgtgc cggcagtgct cgcgtctcct
    20401 tggctcgagg tcgcggtcgt gctgggggcc gccggcttct tcgccttcca gcgcggcctc
    20461 caggtgggcc cggcgattcc cgtcgcggcg ctgatgatgg ccggaacgaa cgtggtcgcc
    20521 gtgctcgcag gcttcctcgt actcggcgac ccgctcggcg ccacgccgtt gctcgcggtg
    20581 gcgcgaatcg ccggcttcgt tgcggtcggg gcggccacgt ggatgctggc tccggcacag
    20641 gtccgcgtta cgcggccgag cctggttgcg actgccgcca cgcgcgagcg atgaacacca
    20701 ccacccggct cggtctcgtc cgccaggacg cggcgctgcg gatcgcgcgc ccggagctgc
    20761 gggcgatcct gacgtcggtg ggcgtactcg ggctcgccgc gtacggcgtg ctctgctggc
    20821 gcatcgtcga ggcgagcttc gtgcaaccgc ggctgctcgt cccgtccgcg cacaggcact
    20881 tccctctctg gatgcgcggc gtgcttcgcg ccaacgggtc accgctgccg tttcacgact
    20941 ttctgaacgt tcttgttgcg ctcacgatgc tgtacgtggt gatcgtcgtg tgcgctcgcg
    21001 cgattcccgc gctgatcggt ctgggcgcgg tggcagcgct cactgtgctc ttcaccctcg
    21061 cgccgccact gctttcgacg gacgtcttca actacatcgc gtactcccgc gccgcccagt
    21121 tgcacgggct gaatccgtac gtgcacggcc cggcggcgct cccgaacgat cccgtctatc
    21181 cgttcgtggg tcacctctgg aaccacgtgc cgagtgcata tggcccgctc ttcacgcttc
    21241 tcacgtacga gctcgagccg ctcggcatcc atggcgagct gtgggcgctc aaggcgatcg
    21301 cgggcgccgc gtgcatcggc tgcgttgcgc tcgtctggct gtgcgccaag cggctcgagg
    21361 ttccggccgt ccccgctgcg ctcttcttcg ggctgaaccc gctcgtgctc gtgtggggcg
    21421 ttggcggagc ccacaacgac atggtgatga cgttcgctct gctgctgggc ctctacctcc
    21481 tgctgtcgcg tcgcccggcc ctcggcggcg ccgccctgac ggtcgcaacc gcggtgaagc
    21541 tcacggctgg gcttgcgctg ccgtttgcga tcgtgggcgt gcgtccccgc tggcgcgtgg
    21601 cggcgggcgc gctcatggcg gcgggcgtgg ggctggcgat cacgtacgcg gagttcgggt
    21661 cccggattcg cggaatgctc gacgcgctca gattccaggg cagcctgcac ttcgtcggcg
    21721 ccaacgttcc cggctatgtc ggctacctgc tcgggcaccc cacggcgggg tacgtgcacc
    21781 ctcacatcct cacggagctc gtcgtggtgg ggtcggcgct gctgcttctc cgcaccgtgc
    21841 ttcagcgatc gtggctcgag tcgacgggct gggcgctgtt gctcctgctg gttaccgcca
    21901 gctggatcct gccttggtac gtcgtgtggg cgctgccgat ggcggcgctc gcgcgcaggc
    21961 cgtggctccg cgctggcgcg gtcgcgctca cgggcgtgct cgtggcgatg cagatcagtc
    22021 acctcgcgct cattcatgac caccatcaca ggcaccggca ccaccaccac aggcaccacc
    22081 acgcgcttgt ccaccatcac accgcgcatg gcccgcgctt cgccgtccgt accggcgggt
    22141 agacgacgac agatgagcga acaccagcca cgcaccgagg aagaggcgga ggaaaccgtc
    22201 gagcagcgcg aggccgagcc cgaggagact ggcgacaagt cgggcgagga cgaaccgggc
    22261 gtgaaccccg agcccgacca gggctacggc ggccagttcg agtagcgctg cggcgccccg
    22321 acgcggctaa tcggcagcga gccgctcctc caccgagcgg accttgtcgt cgagcgtctg
    22381 atccggcttg tcgcggcgct cgtcgagctt caggaccgtg tacacgcgcg ggatgccctg
    22441 ctcgaacggc acggagtgca gctcggccac cacgtcgagg atgtcccgcg tgctgccctc
    22501 gagcgaggtg cccatggcgt gcatgcggta gcgcaccttg tcctgactag cgaggcgacg
    22561 ctggatctcg gcgatgtact cgctcgcgct cacctcgggg cgacccagcg ccagcacggt
    22621 gaggtcggcg gtcgccatct caggcgaggt tggtccagac ggtcttgatc tcgaggtagg
    22681 cgtcgaggtt cgcgtgcccc atctcgcgcc cgatccccga ggccttgacg ccgccgaacg
    22741 gggcagcggc gtcgctcggg ccccagacgt tgatgtagac cgagcccgcc tgcagaagct
    22801 gcgcgagcct gtgcgcgttg cccacgtcgc gggtccagac gccggccgcg aggccgtact
    22861 ccgtgtcgtt cgcacgtcgc gcgacctcct cgaggtcgtc gtagggcatc gcgacgagga
    22921 ccggcccgaa gatctcctcg cgcgcgatcc gcatgtcgtc ctccacaccc gtgaacaggg
    22981 tggggttgat gaagtagccg ttcccgccgt cgccgccgtt gccgcccgcg gccacctctg
    23041 cgccctgctc cttgccggac tcgatgtagc cgatcacgcg ctcgtactgc tccgccgaca
    23101 ccaccgggcc gaactgcgtc gagggatcga ggcccgggcc caccttcgcg ccctttgcgg
    23161 cctcggccag cgcgcccgtg accgcgtcgt agtgctcgcg cggaacgaac aatcgcgagc
    23221 ccgcctggca cgcctggcct gtgttgaagt agatgccctg gaaggcgccc ttgatcgccg
    23281 cgtcgaggtc ggcgtcgggc aggatgatgt tcgggctctt gccgccgagc tcgagcgtga
    23341 cgcgcttcag cgagcggccg cacttctcgc cgatctgacg tccgaccacc gtggatccgg
    23401 tgaaggcgat cttgtcgact cccggatggt cgacgatcgc cgcgccggtc tcgcctccgc
    23461 cggtgacgat gttcacgacg ccctcgggga agcccgcctc ctggatcagc tgccccacgc
    23521 gcagcgcggt gaggggcgtc tgctcggctg gcttcagcac cacggtgcag cccgaggcga
    23581 gcgcgggacc tagcttccaa cccgtcatca gcagcgggaa gttccacgga atgatctgcc
    23641 ccgccacgcc gaccggctcc ttgagcgtgt agacgaacat gttcggccag gagaccggga
    23701 tcgtctcgcc ctccattttg gtgggccagc ccgcgtagta gcgaaggtgc gcgaccgtga
    23761 acggcacgtc cacggcgccc gcgatcgtga ccggcttgcc gttgtcgagc gactcgagct
    23821 gcgccagctc ctcggcgttc ttctcgatca ggtccgcgag cttgttgatc agcagcgaac
    23881 ggcccgccgc aggcatcgat cgccacggtc cctcgaacgc cccgcgcgcg gcactcaccg
    23941 ctcggtccac gtcctcggcg ccggcctcgg cgacggcgca gatcggctgg ccggtcgccg
    24001 ggtcgatcgt ctcgaacgtg cgcccgtcgg ccgcgtccac ccagtcgccg ccgatcagca
    24061 gcct
//
LOCUS       DASVXS010000074         6559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_938289, whole genome shotgun sequence.
ACCESSION   DASVXS010000074 DASVXS010000000
VERSION     DASVXS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938289"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VF752_04330"
     CDS             <1..483
                     /locus_tag="VF752_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854806.1"
                     /translation="GAGQWLTGTSEGVIAKQGDAPYSPGERTGMLKIKRVRTADTVVH
                     AFRFGKEEGTIGSLILALYDDDENLREVGHTSGFTAKQKRELVDKLEPYRTYERGSGE
                     PSRWKSDEELVWEGLRPELVVEVAFDHITGNRIRHGAKFIRWRPDKKPEECRLEQLRA
                     "
     gene            503..2008
                     /locus_tag="VF752_04335"
     CDS             503..2008
                     /locus_tag="VF752_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8854807.1"
                     /translation="MRKAGKLFIAAAFLTLGLAAPAAAYTVPPDNPFVSTAGAKPEIH
                     AYGLRNPFRWSFDRLTGDILIGDVGEGAREEIDYRPASQLAGTNFGWDCWEGTVAGPG
                     PCNPVPPNTPPAYEYPHTSAQILWAVTGGVVARDPGLGALNGRYLFTDFYASQVQSLP
                     FPPTGSPAPTGLPAVSHVVAFGQDAVGHVYVVSLDGQVNRLTSATTSVPVGPSFSAPM
                     YAAAPPNDPHRLFVVERAGRVKLMVDDGAPTTFLDISSQVSTAGEQGLSTIAFPPDYA
                     ASGLFYVYFSDRAGNIHVDQFRRSAANPNAADPGSQQNVLVIPHPGETNHYGGQLNFG
                     GDGYLYVSTGDGGNANDPNGNAQNLGSLLGKLLRIDVRPAGPGNGTVQTQDRVPPRVQ
                     LSARHRQHIGKPRSIVVSARANESATGGAYSLVSVKRARAGRTVRSRTATVRLPANAR
                     KRVRIFFSGKVLRVLRAEVRKRHRTVARVRLTVRDAAGNQSVWRKNVRLVR"
     gene            2038..2529
                     /locus_tag="VF752_04340"
     CDS             2038..2529
                     /locus_tag="VF752_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX8854808.1"
                     /translation="MQLEVLQGDVTTLDVDAIANAANTQLMHGGGVAGAIARAGGPAV
                     QRESHERAPIALGEAVETTAGDMPARWVIHAATMELGGPTSGEIIERATRSTLEKADE
                     LGARSLALVAFGTGVGGFPLDDAARIMTAVVRDYEPRSLERVVFAVRGEDAEQAFRDA
                     ASG"
     gene            2539..3348
                     /locus_tag="VF752_04345"
     CDS             2539..3348
                     /locus_tag="VF752_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8854809.1"
                     /translation="MPIAEVNGQRIYWEEAGEGEPLMLVMGLTADHLAWALQVRELQK
                     DFRVITFDNRDAGQSSECKGEYEVTDMAADAIRLADELGLDSFHLVGASLGGAIAQEM
                     ALSSPERIRTLTLAITWAGAGNWGRKFHRLWSAQVERTPWEEHIDNLMLLVFSEEFFD
                     DQERVTFMRQTMLGNPFPQSKESFTRQVGAAGRHETRERLPHLKMPVHVIGAEHDRLV
                     PVWKSEEIAALIPGAKLTVIEGAAHSVNIERADEFNAAVRDFILAAERKAA"
     gene            complement(3549..3803)
                     /locus_tag="VF752_04350"
     CDS             complement(3549..3803)
                     /locus_tag="VF752_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854810.1"
                     /translation="MATTKQRAAAKRNVKKAQAGARSKQTLKNLPKSTRSALGKEAAA
                     VRRGESPTRAELLDEARKLDIKGRSKMGKAELKRAVSRAK"
     gene            complement(3857..4744)
                     /locus_tag="VF752_04355"
     CDS             complement(3857..4744)
                     /locus_tag="VF752_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX8854811.1"
                     /translation="MNAQVLVDSSLIVFREGLEAVLIFAAVTASMQGANTVHKRPVAA
                     GAGVAFVACVGVWFLFQAVLGAFSEKYGPELQAITGLIAVLVLLVILNWFLHRVYWTG
                     WISHHHKRRRTLLAMPRGGFLSAQVVGLVALGFTSVFREGFEVVLFLQNLQLKAGTPT
                     VMAGTAIGLALTGLVGLATFKLQRRLPYRKMLVVTGALIAFVLVVMIGGTARSFVDLG
                     WLPGTQSDLGVSFPNWLARWLEVVPSVATLSVQAGAAVAVLGSYLVAEHLKVRRPRKR
                     GEQAASRPEQPPVLAEQLA"
     gene            complement(4814..5635)
                     /locus_tag="VF752_04360"
     CDS             complement(4814..5635)
                     /locus_tag="VF752_04360"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX8854812.1"
                     /translation="MAIRCVYTDLDGTLLGRGASLFRDADGAFTLLPARALEACFRAG
                     VEVVIKSGRRRAQVMEDARLLGQTAYIFEVGSGMVIDGEETWLTGDLVPQDGVTIHEQ
                     IEESGAPALLLDAYEGRLEYHSPWHVDREVSHLLRGLVDTEEANTLLEVNGLGHLRLV
                     DNGAISPKESLLGLEGTPHAYHLIPRPASKAGAVEAHMRARGYAREETIAVGDSREDV
                     EVADVVGRFFLVANALEKDPEAVAAAGTRPNAEITEGRNGDGFYEAVVRSLAESR"
     gene            5662..>6559
                     /locus_tag="VF752_04365"
     CDS             5662..>6559
                     /locus_tag="VF752_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8854813.1"
                     /translation="MATVRSKMPWTDLLEAARGEQLVRQSRLGVRPAELRPVPAEVHP
                     ALRDALARTGVTQLYSHQAEALESAFEGHTIVTTGTASGKSLAFNLPVLHTLAGDPSA
                     RALYLYPTKALAQDQARKLHELKAPFLRHAIYDGDTPREERRQIRQRSNLILTNPDML
                     NVGVLPHHRNWGDVLANLAWVVVDEAHVYRGVFGSHVANVLRRLRRLARAYGTEPRFI
                     LTSATIANPLELAEELAGVEFELVDEDGAPRSERQVAMWNPPLLDEQTGARKSSLSEA
                     SDVLARLVEDGVRTICFLKSRKG"
BASE COUNT         1058 a         2293 c         2239 g          969 t
ORIGIN      
        1 ggcgcaggcc agtggctaac cggaacctcc gagggcgtga tcgcgaagca gggcgacgcg
       61 ccttacagcc ccggcgagcg caccggcatg ctgaagatca agcgcgttcg cacggccgac
      121 acggtggtgc acgccttccg cttcggcaag gaagagggca cgatcggctc gctcatcctc
      181 gcgctctacg acgacgacga gaacctgcgc gaggtgggcc acacctccgg cttcacggcc
      241 aagcagaagc gcgagctcgt ggacaagctc gagccctacc gtacctacga gcgcggctcc
      301 ggtgagcctt cgcgctggaa gtccgacgag gaactcgtct gggagggact gcgcccggag
      361 ctcgtcgtcg aggttgcctt cgaccacatc accggcaacc gcatccgcca cggcgccaag
      421 ttcatccgct ggcggccgga caagaagccc gaggagtgcc gcctcgagca gctccgcgcg
      481 tagggcctct accatcctgc cggtgcgcaa ggcagggaag ctcttcatcg ccgcggcgtt
      541 cctgaccctc gggctcgcgg caccggccgc cgcgtacacc gtcccgcctg acaatccgtt
      601 cgtttcgacg gccggcgcga agccggagat tcacgcgtac ggactgcgta atccattccg
      661 ctggtccttc gacaggctga cgggcgacat cctgatcggc gacgtaggcg aaggcgcgag
      721 agaggagatc gactaccgcc ctgcctccca gctcgccggc acgaacttcg gctgggattg
      781 ctgggaggga acggtcgccg ggcctggccc ctgcaatcca gttcccccga acacgccgcc
      841 cgcgtacgag tatccgcaca cctcggccca gatactgtgg gcggtcaccg gtggcgtggt
      901 ggcccgcgat ccggggttag gtgcgctcaa cggcagatat ctcttcactg atttctacgc
      961 gagccaggtg cagtcgcttc cgttcccgcc gacaggctcc ccggcaccaa ccggtctccc
     1021 ggccgtgagc catgtcgtgg cgttcgggca ggacgctgtc ggacacgtat acgtcgtttc
     1081 gctcgatggc caggtcaacc ggctcacctc ggcgacaacg agcgttccgg tcgggccctc
     1141 gttcagcgct ccgatgtacg ccgcggctcc accgaacgac ccacaccggc tcttcgtggt
     1201 cgaacgggcc ggcagggtca agctgatggt ggacgacggc gcgcccacca ccttcctcga
     1261 catctcgagc caagtctcga ccgccggcga gcaggggctc agcacgatcg cgttcccgcc
     1321 tgactacgcg gcatccggtc tgttctacgt ctacttctcc gaccgcgcag gcaacatcca
     1381 cgtcgaccag ttccgtcgtt cggccgcgaa cccaaacgcg gccgatccag ggtcgcaaca
     1441 gaacgtgcta gtcatccctc atccgggcga gacgaaccac tacggcggtc agctgaactt
     1501 cggcggcgac ggctatttgt acgtgtccac gggcgacggt ggcaacgcga atgatcccaa
     1561 cggaaacgcg cagaaccttg gttcgctgct cggcaagctc ctccgcatag acgtccgtcc
     1621 cgcgggtccc ggcaatggga ccgtgcagac acaggaccgt gtgcctcccc gcgttcagct
     1681 ctcggctcgg catcgccagc acatcggcaa gccacgctcg atagtggtat cggcacgggc
     1741 caacgagtca gcgacgggcg gtgcctactc gctcgtgtcc gtgaagcgcg ccagggccgg
     1801 gcggacggtt cggagccgca cggcgacggt gaggctcccc gcgaatgccc gcaaacgcgt
     1861 ccgtatcttc ttctcgggca aagtgctgcg cgtcctgcgt gccgaagtgc gaaagcgcca
     1921 tcgtacggtt gcgcgagtac gcctcacggt gcgcgacgcc gccggcaatc agagcgtctg
     1981 gcgtaagaac gtgcgccttg tgcgctagcg cgcttgcacg tgcgcgacga tattggcgtg
     2041 cagctagagg tgctccaagg cgacgtgacg acgcttgacg tcgacgcgat cgcgaacgcc
     2101 gccaacacgc agctcatgca cggcggcgga gtggccggag cgatcgcgcg agcgggcgga
     2161 ccggcggtgc agcgcgagtc gcacgagcgc gctccgatcg cgctcggcga ggcggtcgaa
     2221 acgactgccg gcgacatgcc ggcgcgctgg gtgatccacg ccgcgacgat ggagctcggt
     2281 gggccgacct cgggcgagat catcgagcgc gccacccgct cgacgctcga gaaggccgac
     2341 gagctcggtg ctcgctcgct ggcgctggtg gcttttggca ccggcgtcgg cggcttcccg
     2401 ctggacgacg ccgcgcgcat catgacggcg gtcgtccgcg actacgagcc gcgttcgctc
     2461 gagcgcgtcg tgttcgcggt gcgcggtgag gacgcggaac aggcgttccg cgacgccgcc
     2521 tccggatagg ctccgccggt gccgatcgcg gaggtcaacg gccagcgcat ctactgggag
     2581 gaagcgggcg agggcgagcc gctgatgctc gtgatggggc tcacagcgga ccacctcgcc
     2641 tgggcgctcc aggtgcgaga gctccagaag gacttccgcg tcatcacctt cgacaaccgc
     2701 gacgcgggcc aatcgtccga gtgcaagggc gagtacgagg tgaccgacat ggcggcagat
     2761 gcgatccggc tcgccgatga gctcggcctc gacagcttcc acctcgtggg tgcgtcactc
     2821 ggcggcgcga tcgctcagga gatggcgctc tcctcaccgg aacggatccg cacgctcacg
     2881 ctcgcgatca cctgggcggg cgccggcaac tggggccgca agtttcaccg gctgtggagc
     2941 gcacaggtgg agcgcacgcc gtgggaggag cacatcgaca acctgatgct cctcgtgttc
     3001 tcggaggagt tcttcgacga ccaggagcgg gtcacgttca tgcgccagac gatgctcggg
     3061 aacccgttcc cgcagtcgaa ggagtcgttc actcgccagg tcggcgcggc cggccgccac
     3121 gagacgcgcg agcggctgcc gcacctgaag atgccggttc atgtgatcgg cgctgagcac
     3181 gaccggctcg tgccggtgtg gaagtcggag gagatcgccg cgctgatccc cggcgccaag
     3241 ctgaccgtga tcgagggcgc cgcccacagc gtcaacatcg agcgcgcgga cgagttcaac
     3301 gcggcggtgc gcgacttcat ccttgcggcc gagcgcaagg cggcttagcc gcagcgacca
     3361 aacggatgag cgccccttgc ggggcgctca ccttgccttg cctgcgtcgc gtagccgctt
     3421 gcgccagcca cgccgcagat gtcaccgcat ccccgccagg gagcggaatc tcgtacctcc
     3481 gatggtacac ccgtcccgtg acgtttgtta caaagatttt gaccgagtcc gcgctcggcc
     3541 gagcgctctt acttcgcgcg cgacaccgcc cgcttcagct ctgccttgcc catcttcgag
     3601 cgccccttga tgtcgagctt gcgcgcctcg tccaagagct cggcccgggt gggggactcg
     3661 cctcgcctca ccgctgccgc ctctttgccg agcgcactgc gagtgctctt gggcaggttc
     3721 ttgagcgtct gcttactgcg tgcgccggct tgcgccttct tgacgttccg ctttgcggct
     3781 gcacgctgct tcgtggtagc catgggcccg agctaccccg gccgcacctc gcgaaacgtc
     3841 gcgctcgagc gtgcgttcac gcgagttgct cggccagcac cggcggctgc tccggtcgcg
     3901 aggcggcctg ctcgccgcgc ttgcgcgggc ggcgcacctt gaggtgctcg gcgacgaggt
     3961 aggagccgag aacggcgacc gcggcaccgg cctgaaccga cagcgtcgcg accgacggca
     4021 ccacctcgag ccagcgcgcg agccagttcg ggaaggagac gccgaggtcg gactgcgtgc
     4081 ccggcagcca gccgaggtcg acgaaggagc gcgccgtgcc gccgatcatc accacgagca
     4141 cgaaggcgat cagcgcgccg gtgaccacga gcatcttgcg gtagggcagc ctgcgctgga
     4201 gcttgaaggt cgcgaggccc acgagcccgg tgagcgccag gccgatcgcg gtgccggcca
     4261 tgacggtcgg cgtgcccgcc ttcagctgca ggttctgcag gaacagcacg acctcgaagc
     4321 cttcgcggaa gacggaggtg aagccgagcg caacgagccc cacgacctgc gccgaaagga
     4381 agccgccgcg cggcatcgcg agcagtgtgc ggcgccgctt gtggtggtgc gagatccagc
     4441 cggtccagta cacgcggtgg aggaaccagt tgaggatcac gagcaggacc agcaccgcaa
     4501 tcaggccggt gatcgcctgc agctcgggcc cgtacttctc ggagaaggcc ccgagcacgg
     4561 cctggaacag gaaccagaca cccacgcatg cgacgaaggc gacaccggcg ccggcagcga
     4621 ccgggcgctt gtgcacggtg ttcgccccct gcatgctcgc ggtgaccgcg gcgaagatga
     4681 ggaccgcctc gagcccctcg cggaagacga tcagcgagga gtcgacgagt acctgcgcgt
     4741 tcaaagccga cctgcggggt aggacatagt tagggcaccc taacaagtcc tccgactcgg
     4801 cgcgtcgccg aagctagcgg ctctccgcga gggagcgcac gaccgcctcg tagaagccgt
     4861 cgccgttgcg cccctccgtg atctcggcgt tcggtcgggt gcccgcggcc gcgacagcct
     4921 cggggtcctt ctcgagcgcg ttcgcaacga ggaagaagcg gccgaccacg tcggcgacct
     4981 ccacgtcctc gcgcgaatcg cccaccgcga tcgtctcctc gcgcgcatac ccgcgcgcgc
     5041 gcatgtgagc ctccacggcc cctgccttcg acgccggccg cgggatcagg tggtacgcgt
     5101 gcggcgtgcc ctcgagcccg agcagggact ccttcgggga gatcgcgccg ttgtcgacga
     5161 ggcgcaggtg cccgaggccg ttgacttcga gcagggtgtt ggcctcctcg gtgtccacga
     5221 gcccacgcag caggtgcgac acctcgcggt ccacgtgcca gggggagtgg tactcgaggc
     5281 gcccctcata cgcgtcgagc agcagcgcgg gcgcgcccga ctcctcgatc tgttcgtgga
     5341 tcgtgacgcc gtcctgcggc acgaggtcac cggtgagcca ggtctcctcg ccgtcgatca
     5401 ccatgccgga gcccacctcg aagatgtagg cggtctgccc gagcaggcgc gcgtcctcca
     5461 ttacctgagc gcggcggcgc cccgacttga tcacgacctc gacgcctgcg cggaagcagg
     5521 cctcgagcgc gcgcgcgggc agcagcgtga aggctccgtc ggcgtcgcga aacagcgacg
     5581 cgccgcgccc gagcagcgtt ccgtcgaggt cggtgtacac gcagcggatg gccattcggg
     5641 cggcgcctac tatggcaacc cgtggccact gtccgctcga agatgccctg gaccgatctg
     5701 ctcgaagccg cgcgcggcga gcagctcgtg cgccagagcc gcctgggcgt gaggccggcg
     5761 gagctccggc cggtccccgc ggaggtccac ccggcgctgc gcgacgcgct tgcgcgcacc
     5821 ggcgtcacgc agctgtactc gcaccaggcg gaggcgctcg agtcggcctt cgagggccac
     5881 acgatcgtca ccaccggcac ggcgagcggc aagtcgctcg cgttcaacct gccggtgctg
     5941 cacacgctgg ccggcgaccc atcggcgcgg gcgctctacc tataccccac gaaggcgctc
     6001 gcccaggacc aggcgcgcaa gctgcacgag ctgaaggcgc cgttcctgcg ccacgcaatc
     6061 tacgacggcg acacgccgcg cgaggagcgc cggcagatcc gccagcgctc gaacctgatc
     6121 ctcacgaacc ccgacatgct gaacgtggga gtgctcccgc accaccgcaa ctggggggac
     6181 gtgctcgcga acctcgcgtg ggtcgtggtg gacgaggcgc acgtgtaccg cggcgtgttc
     6241 gggtcgcacg tggcgaacgt gctgcgcaga ctgaggcgcc tcgcgcgcgc ctacgggacg
     6301 gagccgcgct tcatcctcac gagcgccacc atcgcgaacc cgctcgagtt ggcggaggag
     6361 ctggccggcg tcgagttcga gctcgtcgac gaggacggcg cgccccgctc cgagcgacag
     6421 gtggcgatgt ggaacccgcc gctcctcgac gagcagaccg gcgcgcgcaa gtcgtcgctg
     6481 tccgaggcct cggacgtgct ggcgcggctg gtggaggacg gcgtcaggac gatctgcttc
     6541 ctgaagtcgc gcaagggcg
//
LOCUS       DASVXS010000075        15879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1363028, whole genome shotgun sequence.
ACCESSION   DASVXS010000075 DASVXS010000000
VERSION     DASVXS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363028"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1758)
                     /gene="sufB"
                     /locus_tag="VF752_04370"
     CDS             complement(358..1758)
                     /gene="sufB"
                     /locus_tag="VF752_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8854814.1"
                     /translation="MATTDKTRIQKPGAIAENEQGQLVARKGISREVVEELSKIKNEP
                     QWMRDKRLKSLEIFERKPIPTWGVDLSGLDFDELTLYSPPEAGRYNSWDEVPAEIKQT
                     YEDLGIPQAEREHLAGVVGVWRQEPVYEGLKEEYRKQGIIFCSMDTAVQEYPELVEPY
                     FMTKNVPPQDNKFSALHGAIWSGGSFVYVPKGVKVDLPLQAYFRMEGANEGTFEHTLI
                     IADEGSDVHYIEGCTAQTYSVNSMHSAVVEIFVKEGAKARYTTVQNWSKDVYNLNTKR
                     AVVEANGTVEWVGGSMGAKFVMLYPGSFLLGEGARADHLNVGVASGEVWKDTGAKVMH
                     LAPNTTSNILAKSISKDGGIMGYRGLVRMGHNSQGSKAHVQCDGLMMDEYSRSDTWPD
                     IQIQNPHVTVAHEAVVGKISDEQLFYLQSRGLSEEEAAAMIVNGFIEPVTKELPMEYA
                     VELNRLIQLEMIGSIG"
     gene            1916..2719
                     /locus_tag="VF752_04375"
     CDS             1916..2719
                     /locus_tag="VF752_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854815.1"
                     /translation="MKTTLLAALAALALAANAQADSPASVTATLTDEGAAANGIHTLR
                     VDVAATCPAGTNPWVQADLKGHWKNEVKPPFDPDPRDIDALGADDSVQAASASFKYRL
                     PGGFIASAIGSVQCLDSEGNMTASADSAPTASIRLPVRLAKWESAGTTATFKRASCRV
                     PSRGMQVGAGYDIKWELEADLRGAFGSGAFDIRVADLKQITLHISGGGRRALKFRSSS
                     SGFKHFNGALVNGFYYQPRKAKPVKLWVTVNAVDSNVIAVKVKPKPRGC"
     gene            complement(2716..3219)
                     /locus_tag="VF752_04380"
     CDS             complement(2716..3219)
                     /locus_tag="VF752_04380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8854816.1"
                     /translation="MSDTNGHARKLEIEWEDPRSAAARGLELAGIDYVRGIQAGELPP
                     PPIALLLGMTIVEVEDGRAVFGVRPDERHYNPIGVVHGGLAATLIDSATGCAVQTKLP
                     AGVGYTTVDLNVTFVRPIHADTGDIECEATVIHSGSRVATAEARVRRVSDGKVLAHGT
                     STCLVLG"
     gene            complement(3216..4616)
                     /locus_tag="VF752_04385"
     CDS             complement(3216..4616)
                     /locus_tag="VF752_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8854817.1"
                     /translation="MERRWKVLMVTAAAVFMGFLDVTIVNIAFPDIRGDFNGDSLSSL
                     SWILNAYNVVFAALLIPAGRLADRLGRKRLFMSGLAVFGVASLLCAVAPSVPFLVGAR
                     VVQAAGGALLVPASLGLLLPEFPIEKRATATALWGATGAVAAATGPSLGGLLVDAAGW
                     RWVFLVNVPIAVVALGVGRGLLRESREPSANRLPDPIGVALLGLGVAAVSLGIVKSAD
                     WGWGSTGVVASLAVGAALLIATVARSTSQPAPVFEPALFRVRSFAVANGAIVLFATGF
                     YALLLCNVLFLTQVWHYSVLTAGVMLTPSPIMAAISAAVGGRLADRFGQRVIAVPGLA
                     LFAIGGFLMASVIGAERDYAGELLPIQFVTGIGIGLSFASLSSAAVAELPPMRFATGT
                     AIFGCGRQVGAVLGVALLIAVIGTPAPAEALTAFHHGWELVGATGAAAMLLAAGLGRV
                     RARVPESEVVLAEVGA"
     gene            complement(4619..4786)
                     /locus_tag="VF752_04390"
     CDS             complement(4619..4786)
                     /locus_tag="VF752_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854818.1"
                     /translation="MTPENIPGLRLPRDLTLWTPLQELDITEDEIIAELQSVAGAAPF
                     PVREPAEREAA"
     gene            4885..5349
                     /locus_tag="VF752_04395"
     CDS             4885..5349
                     /locus_tag="VF752_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8854819.1"
                     /translation="MLKRDYDNQVCSIARSLEVIGERWTMLILRDCFVGVRRFDDFQR
                     SLGIARNVLSTRLQRLVDAGILEKRAYEERPVRYEYRLTAKGKDLWPVLMTLSQWGDR
                     YESEAGAPWVFEHRGCGGQVDAHLCCDRCGEPLDPRKLERKPGPGYESIKAA"
     gene            5369..5713
                     /locus_tag="VF752_04400"
     CDS             5369..5713
                     /locus_tag="VF752_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854820.1"
                     /translation="MTAVEAGQVAGVETRDLPATPVGPKKDLARTKLLVSVAVGFAVG
                     LPVAFVASAEFLPLVVWDVTCLVFMAWVWAKIWRADSEETAALAVPEDPSARSPTSCC
                     WSRRWQASWRWA"
     gene            5686..6069
                     /locus_tag="VF752_04405"
     CDS             5686..6069
                     /locus_tag="VF752_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX8854821.1"
                     /translation="MASLVAVGVMLVHAAHSEGLAKGIRAGLGVLSVALSWGLVHTIY
                     TLCYARLYYTGKDGGVDFHGDEPPNYKDFAYLAFTIGMTFQVSDTDLESREIRVDALH
                     HAMLSFVFGTGILATAINLVANLGH"
     gene            6121..7419
                     /locus_tag="VF752_04410"
     CDS             6121..7419
                     /locus_tag="VF752_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX8854822.1"
                     /translation="MALDRPNTVRPITGTSADYDGLLERSLRARYVMIGEASHGTHEF
                     YRHRAELTKRLVDEAGFGAVAVEADWPDAYRVNRYVRDANYDASADEALSDFRRFPVW
                     MWRNTEVADFVGWLRDWNDSVGDDRRKAGFYGLDLYSMHRSMEEVVAYLEVIDPPAAA
                     RARERYSCFDHFGTDPQVYGYETGLGGVEPCEQQAVAQLVVLYRMAADPAMRALLDED
                     RHFYATRNAELVVNAEEYYRSMFRGGVESWNLRDRHMAETLEALASHLRQRQGETKVV
                     VWEHNSHIGDARATELGTTGQFNVGQLEREMYDGEAMLVGCTTYSGAVTAASDWGGVA
                     ERKRVRPALGGSWEELLHEQAGQDMIVETAGMLGPRLERAIGVVYRPETERISHYFHA
                     QLADQFDAVIHIDDTRAVEPLEITSEWERGELPETYPFGV"
     gene            7435..7869
                     /locus_tag="VF752_04415"
     CDS             7435..7869
                     /locus_tag="VF752_04415"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX8854823.1"
                     /translation="MTVGSVDAVERTVHKTNEWLGDMAGELGTDDKEDAWRALSGYLQ
                     VLRDRLTLDEGAQLAAQLPHLLRGVFYEGFDPGHQPEKIRDRDVFLSLLAERAVLADA
                     EEAAVTATAGTRVLRRHVTEGELEDVFVQLPREIRALLEDVR"
     gene            complement(7866..8249)
                     /locus_tag="VF752_04420"
     CDS             complement(7866..8249)
                     /locus_tag="VF752_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX8854824.1"
                     /translation="MDALYRDFILDHYKNPRNFGELEPHDLEAHDHNPLCGDELGVHL
                     RVEDGKITDVAFHGQGCAISQAAASIVSEELEGMSVEEVGQLPASWVIEQLGIEISPT
                     RRKCALLNLKVLRGATTGDASWPEG"
     gene            complement(8261..9505)
                     /locus_tag="VF752_04425"
     CDS             complement(8261..9505)
                     /locus_tag="VF752_04425"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEX8854825.1"
                     /translation="MTTVANRPLSGVRADFPILEREVNGHPLAYLDSAATAQKPRQVL
                     DAMDAYYQASNANVHRGVYELGREATELFEGARERVAEFAGWDPKTTIFTANATAAIN
                     LVAYSWGEAYLSEGDEVVVTEMEHHSNLVPWQEACRRARAKLRYLHVSDEGLLSLDEL
                     DEVLAGGRVKMVAVAHISNVIGTINPVAEIAARAKAAGAVTLVDGSQAVPQLPFSVSD
                     VDADFYAWTGHKALGPTGVGVLHGRRALLESMPPFLSGGHMISSVSAESSTWDELPAK
                     YEAGTSPIAEAVGLGVAVDYLSSVGMENVRAHEAELTAYALERLPEVPGIHIPGPSAD
                     QRGGVVSFGIDGIHPHDIAEICDRFGVCVRAGHHCAQPLMRCIGMPATARASFQVYNT
                     REEVDRLVEALHEARRVFGLTE"
     gene            complement(9505..10800)
                     /locus_tag="VF752_04430"
     CDS             complement(9505..10800)
                     /locus_tag="VF752_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX8854826.1"
                     /translation="MPSPTFLEERRKAALEVFEREPLPTWRRSGFWTTSLRKLRLDEL
                     EPRRYDPVSSLDELNPVVSEALRDEELAGLIVQRGASTVYTQLDPAAAEQGVMVMSLE
                     QALEEHPDLVERWYMKRLSENEGKFPAASAAFWTGGAFVYVPRDVVVDKPIQVVWLID
                     EAGTAQYAHTLGIVEDGSKAYIREYNLAPDFEGQALHAGGFELYCRPSSNVKLAHLQD
                     WGSGEVHDISTKRVEIAADAHCGWVPAVLGGHLTKQTLDIVTHERGSDMRQNGLYFTE
                     GNEHLDIFTTDLHEAGDTTGDTVWKGALTGESRASYEGLIEIVFGAQNSHTYLQTHSM
                     LLSPKAKADSIPSLIVKTDNVSASHGGTVGEVDEEQVFYMMTRGLHRLEAVRILVEGY
                     FEEVIQKLEDDRLEEVVRERIAGKLTEAAEQVEDYIAQR"
     gene            complement(10793..11545)
                     /gene="sufC"
                     /locus_tag="VF752_04435"
     CDS             complement(10793..11545)
                     /gene="sufC"
                     /locus_tag="VF752_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX8854827.1"
                     /translation="MSLEIQNLHVRTEDREILHGVDLVVRKGETHALMGPNGSGKSTL
                     ANTIMGNPNYEVTEGKILLNGEDITEADPDERARAGVFMAFQYPATIPGVSVANFLRM
                     AVNAKRPDDPIKVKEFGQLLRQNMDLLRIDPQFTSRYLNEGFSGGEKKRAEILQLAML
                     KPEFAVLDETDSGLDIDALKIVADGVNALRGPEMGGLIITHYTRILNYVRPDFVHIMI
                     DGRIVREGGPELADHLEEQGYDWIREEVGAGA"
     gene            complement(11542..11988)
                     /locus_tag="VF752_04440"
     CDS             complement(11542..11988)
                     /locus_tag="VF752_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8854828.1"
                     /translation="MFSTKAEYGVRIMAHLAAHDGTAPVALGSIAEAEALPLAYLEHL
                     VQRLRRAGLVESRRGAHGGYTLARPAEEITMAEVVSALEGEIAPIECITADPDGRVMC
                     AREANGERACPSKLLWTRVQGAIVRTLEDVTLSELIRPVARKKAAV"
     gene            complement(12051..12419)
                     /locus_tag="VF752_04445"
     CDS             complement(12051..12419)
                     /locus_tag="VF752_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEX8854829.1"
                     /translation="MKPRIDATDFGSIVVEGNVFDHDVVISPDGEVRKRKKKLSKAVY
                     GTSHTISLEEAKHVYKQAKGADRLIVGAGQYGRVHLSPEAADFFAQKACEIVLLPTPQ
                     VIGIWNETEGRAVGLFHVTC"
     gene            12636..13292
                     /locus_tag="VF752_04450"
     CDS             12636..13292
                     /locus_tag="VF752_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEX8854830.1"
                     /translation="MPDPLVRSRTLLGRSVEKRPITAVELGDPNASHRVLVFGVIHGN
                     EVAGRAITHVLERGPLPRATDLWVVDSLNPDGVAHHTRQNARRVDLNRNFTYRWRPLG
                     RPGDLQYSGRGPLSEPESRIAHSLISRLRPDVTIWFHQPLGVTDLSGGDPQIERRFGR
                     VSGLPVKQLPRYPGSASTWQNHRFRSATAFVVELPPGRGVPGDVARYVRAVRVASSLE
                     "
     gene            complement(13294..14931)
                     /locus_tag="VF752_04455"
     CDS             complement(13294..14931)
                     /locus_tag="VF752_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX8854831.1"
                     /translation="MRLDQTSDQWWKNAVIYCLDVETYFDSDGDGVGDIQGLIGKLDH
                     LAGLGVTCLWLMPFYPTPNRDDGYDIRDHFGVDRRLGTHGDFVELVRTATDRGMRVIV
                     DLVVNHTSDEHPWFVDARRGRDAAHRDWYVWRDEPSDEPKGLAFPGEESSNWQYDEQS
                     GQWYLHRFYHFQPDLDTANPAVRDQIARIMGFWLEVGVSGFRIDAVPSMLETAGLPEH
                     VDDDPQEWLRSLRAFANRRRGDAMLLGEVNVELRQLERYFGEHGDQLHMQFAFLINQH
                     LWLALARGQAEPIEVVVRELPQVPPDNAWVTFLRNHDELTLDKLTLPQREDVFAAFGP
                     DENMRLYGHGLRRRVASMLGGDQDRLRMAWSLLFTLPGTPLLLYGDEIGMGEQLELSD
                     RLSVRTPMQWAPGPSGGFSSARPDELVRPLVDGDFGPDRVSVEAQRRDPDSLLNWMER
                     VIRTRKETPELGWGSSTLLETPAAPLFAHRCDWEGSTVVAVHNLGEGTETATLELGED
                     ATGVDDLLEQRDHELLDGGRLEVKLGRYGYLWLRVRR"
     gene            complement(14934..>15879)
                     /locus_tag="VF752_04460"
     CDS             complement(14934..>15879)
                     /locus_tag="VF752_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854832.1"
                     /translation="WPDMGVLWDLAARTEMTCFGTSAGYIAACMKDGVEPAAARDLSK
                     LRSLGSTGSPLSPEGFDWVYQHVGGDTWLFSTSGGTDVCTAFVGGVPLLPVYRGEIQA
                     RALGAKVEAWDPDGHPLVDEVGELVIAEPMPSMPIFFWNDPDGTRYRESYFDMYPGVW
                     RHGDWIKITSRGTAVIYGRSDSTINRGGVRMGTAEIYRSVLGLDEVTDALVVDVNRGD
                     GSSWMPLFVVLREGLDLDDELRDRIRARLREDCSPRHVPDEISQIAEVPRTLSGKVLE
                     VPVKRILMGADPTKAASRDSLANPAALDYFVELAAARS"
BASE COUNT         2491 a         5434 c         5410 g         2544 t
ORIGIN      
        1 ggccgttggc ctgcgactgg atcgtgaagc aacggttggc gagctgagga ccggcaccgg
       61 cggcggcgct cgccggtgca gacgacgccg caacagctcc tgcggcgacc aacaagccag
      121 cggcgcgccg agcaaacgat cccctcgtca tgcggcggca gcataaaggc gcgggcgacg
      181 gacgagagtg catttgaccc cgatacacgg ggttgacgtc cgcctcgttc cggcgcagca
      241 ccccaactcc tgactttggt tctcgtatgc ggaaccaaac tcaggtggtc acccgtgtgc
      301 caaacgaaac ggggcgccga tggcgccccg ttcaaatgct gcgttcggaa ggcggcgtta
      361 gccgatcgag ccgatcatct cgagctggat caagcggttc agctcaaccg cgtactccat
      421 cggcagctcc ttcgtgaccg gctcgatgaa gccgttcacg atcatcgcgg ctgcctcctc
      481 ctcgctgagc ccgcggctct ggaggtagaa cagctgctcg tcggagatct tgccgacgac
      541 cgcctcgtgc gccaccgtga cgtgcgggtt ctggatctgg atgtcaggcc acgtgtccga
      601 gcgcgagtac tcgtccatca tcaggccgtc gcactgcacg tgggccttcg agccctgtga
      661 gttgtggccc atgcggacga gcccgcggta gcccatgatc ccgccgtcct tcgagatgga
      721 cttcgcgagg atgttcgacg tggtgttcgg cgcgaggtgc atgaccttgg cgccggtgtc
      781 cttccagacc tcgccgcttg cgacgcccac gttcaggtgg tcggcacgcg cgccctcacc
      841 gagcaggaac gagcccgggt agagcatcac gaacttggcg cccatcgagc cgccgaccca
      901 ctccaccgtg ccgttggcct ccacgacagc gcgcttggtg ttcaggttgt agacgtcctt
      961 cgaccagttc tggacggtcg tgtagcgggc cttcgcgccc tccttcacga agatctcgac
     1021 gacggccgag tgcatcgagt tcacgctgta ggtctgcgcc gtgcagccct cgatgtagtg
     1081 aacgtcggag ccctcgtcgg cgatgatcag cgtgtgctcg aaggtgccct cgttggcgcc
     1141 ctccatgcgg aagtaggcct gcagcggcag gtccaccttc acgcccttcg ggacgtacac
     1201 gaacgagccg cccgaccaga tcgcgccgtg gagcgccgag aacttgttgt cctgcggcgg
     1261 cacgttcttc gtcatgaagt acggctcgac gagctcgggg tactcctgca ccgccgtgtc
     1321 catggagcag aagatgatcc cctgcttgcg gtattcctcc ttgaggccct cgtacaccgg
     1381 ctcttgccgc cacacgccga cgacgccggc caggtgctcg cgctcggctt gcgggatccc
     1441 gaggtcctca taagtctgct tgatctccgc gggaacctcg tcccaggagt tgtagcgccc
     1501 agcctcgggc gggctgtaga gggtcagctc gtcgaagtcg agcccggaga gatcgacgcc
     1561 ccaggtgggg atgggcttgc gctcgaagat ctcgagcgac ttcagcctct tgtcccgcat
     1621 ccactgcggc tcgttcttga tcttcgacag ctcctccacg acctcgcgcg agatgccctt
     1681 gcgcgcgacg agctggccct gctcgttctc cgcgatcgcg ccgggcttct ggattcgcgt
     1741 cttgtcagtg gttgccatct aattcctcgt tccgaagtcg gtattcgctc cgaatgatag
     1801 agatcgccgg agccggaccc atcgaaacgg ggtctggcgc gcaggtgcca cgttcggtgc
     1861 gcgcgcgtac ctcgattgtg gcgaagggca cgcttcgggc ttaggatcgg cccgcatgaa
     1921 gaccaccctc ctcgccgctc tcgcggcgct cgccctcgcc gcgaacgccc aagccgactc
     1981 cccggcctcc gttaccgcca cgctcacgga tgagggagcc gccgcgaacg gcatccacac
     2041 cctgagggtc gacgtggccg ccacctgccc cgccggcacg aacccctggg tgcaggccga
     2101 cctcaagggg cactggaaga acgaggtcaa gccgccgttc gaccccgacc cgcgggacat
     2161 cgacgccctc ggtgccgacg actccgtgca ggccgcgagc gcctccttca agtaccgcct
     2221 tccgggcggc ttcattgcga gcgcgatcgg ctcggtgcag tgcctcgact ccgagggcaa
     2281 tatgacggct tcggccgact ccgcgcccac cgcgtccatc cggctgccgg tgcgcctggc
     2341 gaaatgggag tccgccggta caacggcaac cttcaagagg gcgagctgca gggtcccgtc
     2401 ccgcggcatg caggtgggag cgggctacga catcaagtgg gagctcgagg cggatctcag
     2461 gggcgcgttc ggctcaggcg cgttcgacat ccgcgtagcc gacctgaagc agatcacgct
     2521 gcatatctcc ggtggcggca ggcgtgcgct gaagttccgc tcgagcagca gcggcttcaa
     2581 gcatttcaac ggcgcgctcg tgaacggctt ctactaccag ccgcgcaagg ccaagccggt
     2641 gaagctctgg gtgaccgtga acgcggtgga ctcgaacgtc atcgcggtga aggtaaagcc
     2701 caaaccgcgc ggctgctagc caaggacgag gcaggtgctc gtgccatgcg cgagcacctt
     2761 gccgtcgctg acccggcgca cgcgcgcctc ggcggtggcg acgcgggagc ccgagtggat
     2821 caccgtcgcc tcgcactcga tgtcgccagt gtccgcgtgg atcggtcgca cgaaggtcac
     2881 gttgagatcg accgtcgtgt agcccacgcc cgccggcagc ttggtctgca cggcgcagcc
     2941 ggtggcggaa tcgatcagcg tcgctgccag gccgccgtgc acgaccccaa tcgggttgta
     3001 gtggcgctcg tcgggccgaa cgccgaacac ggcgcggccg tcctcgacct cgacgatcgt
     3061 catgccgagc agcagcgcga tcggcggggg cggcaactcg cccgcctgga tcccgcgcac
     3121 gtagtcgatg cccgcgagct cgaggccgcg ggcggcggcg ctgcgcggat cctcccactc
     3181 gatctcgagc ttgcgcgcgt ggccgttcgt gtcgctcacg cgcccacctc ggcgagcact
     3241 acctcgcttt ccggaacgcg ggcgcgcacg cgccccagcc cggcggcgag cagcatggcg
     3301 gcggcgccgg tcgcgccgac cagctcccat ccgtggtgga aggcggtcag ggcctcggcc
     3361 ggcgcgggcg tgccgatcac ggcgatcaga agcgccactc cgagaaccgc gccgacctga
     3421 cggccgcagc cgaagatcgc ggtgccggtt gcaaagcgca tcggcgggag ctcggccact
     3481 gccgcactcg acagcgacgc gaagctgagc ccgattccga tgcccgttac gaactggatt
     3541 ggcagcagct cgccggcata gtcgcgctcg gcgccaatca cactcgccat gagaaagccg
     3601 ccgatcgcga agagcgccag ccccggcacc gcgatcaccc gctgcccgaa gcggtcagcg
     3661 agccggccgc ccacggccgc cgagatggcg gccatgattg gtgagggcgt gagcatcacg
     3721 ccagcggtga gcaccgagta gtgccacacc tgggtcagga agaggacgtt gcagagcagc
     3781 agggcgtaga agccggtcgc gaacagcacg attgcgccgt tggcgacggc gaaggagcgc
     3841 acgcggaaca gcgcgggctc gaagaccggt gccggctgag acgtcgagcg cgccaccgta
     3901 gcgatgagca aggcggcacc gacggcaagc gaagcaacga cgcccgtgct gccccagccc
     3961 cagtcggcgc tcttcacgat gccgagcgaa acggccgcga cgcccaggcc gagcagcgcc
     4021 acgccgatcg ggtcgggcag gcggttggcg gagggctcgc ggctctctcg gagcaggcct
     4081 cgccccacgc cgagcgcgac cacggcgatc ggcacgttca cgaggaacac ccagcgccag
     4141 cccgcggcgt ccacgaggag cccgccgagc gacgggccgg tcgccgcggc caccgcgccg
     4201 gttgcgcccc agagcgcggt ggcggtggcc cgcttctcga tcgggaactc aggcagcagc
     4261 aggccgagcg acgccggcac gagcagcgcc ccgcccgcgg cctgcaccac ccgcgcgccg
     4321 acgaggaacg gaacggacgg cgctacggca cagagcagcg acgccacgcc gaagacggcg
     4381 aggccgctca tgaaaagccg cttgcggccg aggcggtccg ccagccggcc cgcggggatg
     4441 agcagcgcgg cgaacaccac gttgtaggcg ttcaggatcc aggagaggga ggagagcgag
     4501 tcgccgttga agtcgccgcg gatgtccggg aaggcgatgt tgacgatcgt cacgtcgagg
     4561 aagcccatga acacggccgc agccgtgacc atgaggacct tccagcggcg ctccatcttc
     4621 aggccgcctc gcgctcagcg ggctcgcgaa ccgggaacgg cgccgcgccg gcgaccgact
     4681 ggagctcggc aatgatctcg tcctcggtga tgtcgagctc ctggagcgga gtccacagcg
     4741 tcaggtcgcg ggggaggcga aggccgggga tgttctcggg agtcatggcg tgctccagtc
     4801 ggtccggaga tgggttgctt agagaaacgc actattgcag agtcagttgc atcacgcaac
     4861 ctgcgtgact acaatcacgt atcgatgctg aagcgcgact acgacaacca ggtctgctcc
     4921 atcgcccgct cgctggaggt gatcggggag cgctggacga tgctgatcct ccgcgactgc
     4981 ttcgtcggtg tgcggcgctt cgacgacttt cagcgcagcc tcggcatcgc gcgcaatgtg
     5041 ctgagcacgc ggctgcagcg gctggtggac gccgggattc ttgagaagcg cgcgtacgaa
     5101 gagcgtcctg tgcgctacga gtaccgtctc actgccaagg ggaaggacct atggcccgtc
     5161 ctgatgaccc tgagccagtg gggtgaccgc tacgagtccg aggccggcgc gccgtgggtc
     5221 ttcgagcacc ggggctgcgg cggtcaggtg gacgcgcatc tctgctgcga tcgctgtggc
     5281 gagccgctcg atccgcgcaa gctggagcgc aagccgggcc cgggctacga gagcatcaag
     5341 gccgcctgac ccttaccaag tctctaggtt gaccgccgta gaggccgggc aagtggccgg
     5401 cgtggagaca cgtgatcttc ccgccacgcc cgtcgggccg aagaaagacc tcgcacgcac
     5461 aaagctgctc gtgtccgtcg ccgtcggctt cgcggtggga ctccccgttg cctttgtcgc
     5521 gtcggccgag ttcctgccgc tggtcgtctg ggacgtgacg tgcctcgtct tcatggcctg
     5581 ggtctgggca aagatctggc gcgctgacag cgaggagacc gccgccctcg ccgtgcccga
     5641 ggacccgagc gcgcgttcgc cgacctcctg ctgctggtcg cgtcggtggc aagcctcgtg
     5701 gcggtgggcg tgatgctcgt gcacgccgcc cacagtgagg gcctcgcgaa gggaatccga
     5761 gccggcctcg gcgtgctctc ggtggcgctc tcttggggcc tcgtgcacac gatctacacg
     5821 ctctgctacg cccgcctcta ctacacgggc aaggacggcg gagtggactt ccacggcgac
     5881 gagcctccga actacaagga tttcgcctac ctcgcgttca cgatcgggat gacgttccag
     5941 gtctccgata cggacctgga gagtcgcgag atccgcgtgg acgcgctgca ccacgcgatg
     6001 ctgtcgttcg tgttcgggac gggcatcctc gcaacggcga tcaacctggt cgcgaatctc
     6061 ggccactagc ggttgaccgc ctcggtctgc gccttactca cccagaaggg aggcaagcgc
     6121 ttggctctgg atcgacccaa caccgtccgg cccataacgg gcacctccgc cgattacgac
     6181 ggcctgctcg agcgatcgct gcgagcccgc tacgtgatga tcggcgaggc ctcacacggc
     6241 acgcacgagt tctaccggca tcgcgccgag ctcaccaagc gcctggtcga cgaggcaggc
     6301 ttcggcgccg tcgcggtcga ggccgactgg cccgacgcgt atcgagtgaa ccgttacgtg
     6361 cgagacgcca actacgacgc gagcgccgac gaggcgctca gcgatttccg ccgtttcccc
     6421 gtgtggatgt ggcgtaatac cgaggtggcc gacttcgtcg gctggctgcg cgactggaac
     6481 gactcggttg gcgacgaccg ccggaaggcc gggttctacg ggctcgatct ctacagcatg
     6541 caccgctcga tggaggaggt cgtcgcctat ctcgaggtga tcgacccgcc ggcggcggcg
     6601 cgtgcgcgtg agcgctattc ctgcttcgac cacttcggca ccgacccgca ggtctacggc
     6661 tacgagaccg ggctaggcgg cgtcgagccg tgcgagcagc aggcggtcgc ccagctcgtg
     6721 gtgctgtatc gcatggcggc cgatccggca atgcgggcgc tgctcgacga ggatcgccac
     6781 ttctacgcca cgcgcaacgc cgagctcgtg gtgaatgcgg aggagtacta ccgctccatg
     6841 ttccgcgggg gcgtggagag ctggaacctg cgcgaccggc acatggccga gacgctcgag
     6901 gcgctcgcca gccacctgag gcagcggcag ggcgagacca aggtggtcgt gtgggagcac
     6961 aactcgcata tcggcgacgc gcgggccacc gagctgggaa cgactggtca gttcaacgta
     7021 ggccagctcg agcgggagat gtacgacggc gaggcgatgc tcgtcggctg cacgacctat
     7081 tccggggccg tcaccgccgc gtcggactgg ggcggcgtcg ccgagcgcaa gcgcgtgcgc
     7141 ccggcactcg gcggtagctg ggaggagcta ctgcatgagc aggccggcca ggacatgatc
     7201 gtcgagacgg cggggatgct cgggccgcgg ctcgagcgcg ccatcggggt cgtgtaccgg
     7261 ccagagaccg agcgcatctc ccactacttc cacgcgcagc tggcggatca gttcgacgcg
     7321 gtgatccaca tcgacgacac gcgggcggtc gagccgctcg agatcacgag cgaatgggag
     7381 cgcggcgagc tgccggagac gtacccattc ggcgtgtagg cgagaggagg tcttatgacg
     7441 gttggaagcg ttgacgccgt ggagcgcacg gtgcacaaga cgaacgagtg gctcggcgac
     7501 atggccggcg agctcggaac agatgacaag gaagatgcct ggcgcgcgct gagcggctac
     7561 ctgcaggtgc tgcgcgaccg cttgaccctg gatgagggcg cccagctggc ggcgcagctt
     7621 cctcacctgc tgcgcggcgt gttctacgag ggcttcgacc ccggccacca gccggagaag
     7681 atccgcgatc gtgacgtctt cctgagccta ctggccgagc gcgcggtact tgccgatgcc
     7741 gaggaggccg ccgtcacggc aaccgcgggg acgcgcgtgc tccgccgcca cgtgaccgag
     7801 ggcgagttgg aagacgtgtt cgtgcagctc ccgcgggaga ttcgggcgct actcgaggat
     7861 gtgcgctagc cctcgggcca ggacgcgtcg ccggtagtgg cgccgcgcaa gaccttgagg
     7921 ttgagcagcg cgcacttgcg gcgcgtggga gagatctcga tgccgagctg ctcgatcacc
     7981 cagctcgccg gaagctggcc gacctcctcg acgctcatgc cctcgagctc ctcggaaact
     8041 atcgaggcgg ccgcctgcga gatcgcgcag ccctgcccgt ggaaggcgac gtcggtgatc
     8101 ttgccgtcct cgactcgcag gtgcacgccg agctcgtcgc cgcacagcgg attgtggtca
     8161 tgcgcctcga ggtcgtgcgg ctcgagctcc ccgaaattcc gggggttctt gtagtgatcc
     8221 aagatgaagt ctcggtacag cgcgtccatc gccatcgacg ttactcagtg agtccgaata
     8281 cccgtcgcgc ttcgtggagc gcctcgacca ggcggtccac ctcttcgcgc gtgttgtaga
     8341 cctggaacga ggcccgcgcg gtcgccggca tgccgatgca gcgcatcagc ggctgggcgc
     8401 agtggtggcc cgctcgcacg cacacgccga agcggtcgca gatctccgcg atgtcgtgcg
     8461 ggtggatgcc gtcgatgccg aacgagacga cgccgccgcg ctggtcggcg gacggccccg
     8521 ggatgtggat gccgggcacc tccggcaggc gctcgagcgc gtacgccgtc agctccgcct
     8581 cgtgcgcgcg cacgttctcc atcccgaccg acgacaggta gtcgaccgcc acgccgagcc
     8641 cgaccgcctc cgcgatcggc gaagtgccgg cctcgtactt cgccggcagc tcgtcccagg
     8701 tggagctctc ggcgctgacc gacgagatca tgtggccgcc cgacaggaac ggcggcatcg
     8761 actcgagcag cgcgcggcgc ccgtgaagca ccccgacgcc ggtcgggccg agggccttgt
     8821 ggccggtcca ggcgtagaag tcggcgtcca cgtcagacac gctgaacggc agctgaggca
     8881 ccgcttggga gccgtccacg agcgtgacgg cgccggctgc cttggcgcgg gcggcgatct
     8941 cggcgacggg gttgatcgtt ccgatcacgt tcgagatgtg cgcgaccgcg accatcttca
     9001 cgcggccccc tgcgagcacc tcgtcgagct cgtcgagcga gagcaggccc tcgtcggaga
     9061 cgtgcaggta gcgcagcttg gcgcgggcgc gccggcacgc ctcctgccac ggcacgaggt
     9121 tcgagtggtg ctccatctcg gtgacgacga cctcgtcgcc ctcggacagg tacgcctcgc
     9181 cccacgagta cgcgaccagg ttgatcgcgg ccgtggcgtt cgcggtgaag atcgtggtct
     9241 tcggatccca gccggcgaac tccgcgacgc gctcgcgcgc gccctcgaac agttccgtcg
     9301 cctcgcggcc gagctcgtac acgccgcggt gcacgttcgc gttcgacgcc tggtagtagg
     9361 cgtccatcgc gtcgagcacc tggcgcggct tctgcgcggt cgccgccgag tcgaggtagg
     9421 ccagcgggtg accgttcacc tcgcgctcga ggatcgggaa gtcggcgcgc acgccggaca
     9481 ggggcctgtt cgcaaccgtg gtcatcagcg ctgcgcgatg tagtcctcga cctgctcggc
     9541 cgcctcggtg agcttgcccg cgatccgctc gcgcacgacc tcctcgagcc ggtcgtcctc
     9601 gagcttctgg atgacctcct cgaagtagcc ctcgacaagg atccgcaccg cttcgaggcg
     9661 gtgcaggcca cgggtcatca tgtagaagac ctgctcctcg tcgacctcgc cgacggtgcc
     9721 gccgtgcgac gccgacacgt tgtcggtctt cacgatcagc gatgggatcg agtcggcctt
     9781 cgccttgggt gagagcagca tcgagtgcgt ctgcaggtac gtgtgggagt tctgcgctcc
     9841 gaacacgatt tcgatcaggc cctcatacga ggcgcgcgac tcgccggtga gagcgccctt
     9901 ccagacggtg tcgccggtgg tgtcgccggc ctcgtggagg tcggtcgtga agatgtcgag
     9961 gtgctcgttg ccctcggtga agtagagccc gttctggcgc atgtccgaac cgcgctcgtg
    10021 ggtcacgatg tcgagcgtct gcttcgtgag gtgaccgccg agcacggccg ggacccaacc
    10081 gcagtgagcg tccgccgcga tctccacgcg cttcgtggag atgtcgtgca cctcgccgct
    10141 gccccagtcc tggaggtggg cgagcttcac gttcgagctc gggcggcagt acagctcgaa
    10201 gccgcccgca tggagcgcct ggccctcgaa gtcgggcgcg aggttgtact cgcggatgta
    10261 ggccttcgac ccgtcctcga cgatcccgag cgtgtgcgcg tattgcgccg tgccggcctc
    10321 gtcgatcagc cacacgacct gaatcggctt gtcgacgaca acgtcgcggg gcacgtacac
    10381 gaacgcgccg ccggtccaga aggccgctga ggcggcgggg aacttgccct cgttctctga
    10441 gagccgcttc atgtaccagc gctcgacgag gtccgggtgc tcctcgagag cctgctccag
    10501 cgacatcacc atcacaccct gctcggccgc tgccgggtcg agctgcgtgt agacggtgct
    10561 cgcgccgcgc tggacgatca ggccggcgag ctcctcgtcg cgcagcgcct cggacacgac
    10621 cggattcagc tcgtcgagcg acgagacggg gtcgtaccgc cgcggctcga gctcgtcgag
    10681 gcggagcttg cgcagcgacg tggtccagaa gcccgaacgc cgccaggtgg gcagcggctc
    10741 gcgctcgaac acctcgagcg cggccttccg cctctcctcg aggaaggtgg ggctaggcac
    10801 cggcgccgac ctcctcgcgg atccagtcgt atccctgctc ctcgaggtgg tcggccagct
    10861 cggggccgcc ctcgcgcacg atcctgccgt cgatcatgat gtggacgaag tccgggcgca
    10921 cgtagttcag gatgcgcgtg tagtgcgtga tgatcaggcc gcccatctcc gggccgcgca
    10981 gcgcgttgac gccgtcggcg acgatcttca gggcgtcgat gtcgagaccg gagtcggtct
    11041 cgtcgagcac cgcgaactcc ggcttgagca tcgccagctg gaggatctcg gcgcgcttct
    11101 tctcaccgcc tgagaagccc tcgttcaggt agcggctcgt gaactgcggg tcgatacgca
    11161 gcaggtccat gttctggcgc aggagctggc cgaactcctt gaccttgatc gggtcgtcgg
    11221 ggcgcttggc gttcacggcc atgcgcagga agttggccac cgagacgccc gggatggtgg
    11281 ccgggtactg gaacgccatg aacacgcccg cgcgtgcacg ctcgtcgggg tccgcctcgg
    11341 tgatgtcctc cccgttcagg aggatcttgc cctcggtgac ctcgtagttc gggttgccca
    11401 tgatggtgtt ggcgagcgtc gacttgcccg agccgttcgg gcccatcagc gcgtgtgtct
    11461 cgcccttgcg cacgacgagg tcgaccccgt ggaggatctc tcggtcctcg gtgcgcacgt
    11521 gaaggttctg gatttccagg ctcatacagc cgctttcttc ctcgctactg gtcggatcag
    11581 ttcggacagg gtcacgtcct cgagcgttct cacgatggcg ccctgaacgc gcgtccacag
    11641 cagcttgctt ggacaggcgc gctcgccgtt cgcctcacgg gcgcacatca cgcggccgtc
    11701 gggatcggcc gtgatgcatt cgatcggggc gatctcgccc tcgagcgccg agacgacctc
    11761 ggccatcgtg atctcctccg ccggtcgcgc gagcgtgtag ccgccgtggg cgccccggcg
    11821 cgactccacg agcccggcgc ggcgaagacg ctgcaccaga tgctcgaggt acgcgagcgg
    11881 cagcgcctcg gcctcggcga tggagccgag cgccacgggc gccgtgccgt cgtgtgcggc
    11941 gaggtgcgcc atgatgcgca cgccgtactc ggcctttgtc gagaacatca tcggtgaata
    12001 cctacctgga aggtaggata cgcacccgtg cggaggggcc tacccgcccc tcagcaagtc
    12061 acgtggaaga gaccgaccgc cctgccctcg gtctcgttcc agatcccgat cacctgaggg
    12121 gtcggcagca gcacgatctc gcacgccttc tgggcgaaga agtcggccgc ctccggcgag
    12181 aggtgcaccc tgccgtattg gccggcgccg acgataaggc ggtctgcccc ctttgcctgc
    12241 ttgtagacgt gcttggcctc ttcaagcgag atcgtgtgcg aagtcccgta gaccgccttc
    12301 gagagcttct tcttgcgctt tcttacctcg ccgtccggac taatgacgac gtcgtggtcg
    12361 aagacattcc cctcgaccac gatcgagccg aagtccgtcg cgtcgatccg aggcttcatc
    12421 tcgcacctcc aatttgccag cgagccagca tccgggaccc gcggtgcagt ggatgccccg
    12481 agaggcataa cgcgcgcgcg acacgacgtc aacgctccgg gtgtggttcg ccaacgccca
    12541 gcgataatcg atccgtgcgg cgagtcgccg gcgcgctagt gctcgcaacc ctcttggttg
    12601 cttcctcggg tttcactgga cgccgagacc ctgccgtgcc ggacccgctc gttcgcagcc
    12661 gcacgctgct cgggcgctcg gtggagaagc gcccgatcac agccgtcgag ctcggtgacc
    12721 cgaacgcatc gcaccgcgtg ctcgtgttcg gtgtgatcca cggaaacgag gtcgccgggc
    12781 gggcgatcac gcacgtgctc gagcgcgggc cgctcccgag ggcgaccgac ctctgggtgg
    12841 tcgacagcct caacccggac ggcgtcgcgc accacacccg ccagaacgcg cgccgggtgg
    12901 acctcaaccg caacttcacc taccgctggc gcccgctggg ccggcccggc gatctccagt
    12961 attcggggcg cggtccgctc tccgagcccg agagccgcat cgcgcactcg ctgatctcac
    13021 ggctgcgacc cgacgtgacg atctggttcc atcagccgct cggcgtcact gacctctccg
    13081 gcggcgatcc ccagatcgaa cggcgcttcg ggcgggtcag cgggttgccg gtgaagcagc
    13141 tgccccgcta tcccggtagc gccagcacct ggcagaacca tcgcttccgc tcggccacgg
    13201 cgttcgtggt ggagttgccg cccgggcgag gggtgcccgg tgacgtggcg cgctacgtgc
    13261 gcgccgtgcg agttgccagc tcgttggagt gacctagcgc cgcacgcgca gccacaggta
    13321 gccgtagcgg ccgagcttca cttcgaggcg tccgccgtcg agcaactcgt ggtcgcgctg
    13381 ctcgagcagg tcgtcaacac cggtcgcgtc ctcgccgagc tcgagcgtcg ccgtctcggt
    13441 gccctcgccg aggttgtgca cggccacgac ggtgcttccc tcccagtcgc agcggtgggc
    13501 gaacagcggc gcggctgggg tctcgaggag cgtgctcgag ccccagccca gctcgggggt
    13561 ctccttgcgc gtccggatca cgcgctccat ccagttgagc agcgaatcgg ggtcgcgccg
    13621 ctgcgcttcc acgctcaccc gatccggacc gaagtcgcca tcaaccagcg ggcgcacgag
    13681 ctcgtccggg cgcgcgctcg agaagccccc gctcgggccg ggcgcccact gcatcggcgt
    13741 gcgcacgctg agccggtcgg acaactcgag ctgctcgccc atcccgatct cgtcgccgta
    13801 gagcagcagc ggcgtgccgg gaagtgtgaa cagcaggctc caagccatcc gcagccggtc
    13861 ctggtcgccg cccagcatgg aggcgacccg ccggcgcagg ccgtgcccgt acaggcgcat
    13921 gttctcgtcg ggcccgaagg ccgcgaacac gtcctcacgc tgcggcagcg tgagcttgtc
    13981 gagcgtcagc tcgtcgtggt ttcgcagaaa cgtgacccag gcgttgtccg gcggcacctg
    14041 tggcagctcc cggaccacca cctcgatcgg ctcggcctgg ccgcgagcca gcgcgagcca
    14101 caggtgctgg ttgatgagga acgcgaactg catgtgcagc tggtcgccat gctcgccgaa
    14161 gtagcgctcg agctggcgca gctccacgtt cacctcgccg agcagcatcg cgtcgccgcg
    14221 acgacggttc gcgaacgcgc gcaggctgcg cagccactcc tgcgggtcgt cgtcgacgtg
    14281 ctcgggcagg cccgcggtct cgagcatcga cgggaccgcg tcgatccgga agcccgatac
    14341 gccgacctcg agccagaagc ccatgatgcg cgcaatctgg tcgcgcaccg cgggattggc
    14401 cgtgtcgaga tcaggttgga agtggtagaa gcggtgcagg taccactggc cggactgctc
    14461 gtcgtactgc cagttgctcg attcctcgcc ggggaacgcg agccccttcg gctcgtccga
    14521 cggctcatcg cgccacacgt accagtcgcg atgcgcggcg tcccggcccc ggcgcgcgtc
    14581 gacgaaccat gggtgctcgt ccgacgtgtg gttcacgacg aggtcaacaa tcacgcgcat
    14641 cccgcggtcc gtcgccgttc gtaccagctc gacgaaatcg ccgtgggttc ccaggcgccg
    14701 gtcgacaccg aagtggtcgc ggatgtcgta gccgtcgtcg cggttcggag tggggtagaa
    14761 gggcatgagc cagaggcacg tgacgcccag ccccgcgagg tggtccagct tgccgatcag
    14821 gccctggatg tcaccgacac cgtcaccgtc ggagtcgaag tacgtctcga cgtccaggca
    14881 gtagatgacg gcgttcttcc accactggtc gctcgtctgg tcgagtcgca ccgctagctc
    14941 cgcgcggccg ccagctctac gaagtagtcg agcgcagcgg ggttcgcgag cgagtcgcgg
    15001 ctggcggcct tcgtggggtc ggcgcccatc aggatccgct tcaccggcac ctccagaacc
    15061 ttcccgctca gcgtgcgcgg cacctcggcg atctgcgaga tctcgtcggg cacgtgccgc
    15121 ggcgagcagt cctcgcggag gcgggcgcgg attcggtccc gcagctcgtc gtcgaggtcg
    15181 agtccttcgc gcagaacgac gaacagcggc atccaggacg agccgtcgcc gcggttcacg
    15241 tcgaccacga gcgcgtcggt cacctcgtcg agcccaagca ccgagcggta gatctctgcg
    15301 gtgcccatcc gtacgccgcc gcggttgatg gtggagtcag agcgcccata gatcacggcg
    15361 gtgccgcgcg aggtgatctt gatccagtcg ccgtggcgcc acacgccggg gtacatgtcg
    15421 aagtagctct cgcggtagcg cgtgccgtcc gggtcgttcc agaagaagat cggcatcgat
    15481 ggcatcggct cggcgatcac gagttcgccc acctcgtcca cgagcggatg accgtcaggg
    15541 tcccaggcct cgaccttggc gccgagcgcc cgcgcctgaa tctcgccgcg gtacacgggc
    15601 agcagcggca cgccgcccac gaacgccgtg cagacatcgg taccgcccga ggtcgagaag
    15661 agccaggtgt caccgccgac gtgctgatac acccagtcga agccctcggg cgagagcggc
    15721 gagccggtcg agcccaggct acgaagcttc gagaggtcgc gcgcggcggc tggttcgacg
    15781 ccgtccttca tgcatgcggc gatgtacccg gccgaggtgc cgaagcaggt catctcggtg
    15841 cgcgctgcga gatcccacag cacacccatg tccggccag
//
LOCUS       DASVXS010000076        15025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1000476, whole genome shotgun sequence.
ACCESSION   DASVXS010000076 DASVXS010000000
VERSION     DASVXS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15025
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000476"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF752_04465"
     CDS             complement(<1..388)
                     /locus_tag="VF752_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8854833.1"
                     /translation="MKRELGDGYELDDDPARIDIDAVFRYLSGESYWARGRSRETVER
                     LVREAQRVVGIYKEGRQVGFSRTVTDGVAFAYLADVYILPEHQGRGLGKELVRETVVL
                     GPYSKRRWLLHTYDAHDLYRQFGFGKP"
     gene            complement(385..1167)
                     /locus_tag="VF752_04470"
     CDS             complement(385..1167)
                     /locus_tag="VF752_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8854834.1"
                     /translation="MSQPRLSERLGIAGSGAIATGLARTAAPFGDVVLWARSDDSADR
                     AQGRVDDGVRVVTDLGELDGSTMIVEAIVEDPEAKAEMLQRLHDLAHEDTVIASTTSS
                     LSISELADASGRPERVVGLHVFNPVEKMKLVELAFPNAASEDTRRRARAFCDELGKTP
                     VEVPDTPGFVVNRLLFPYLFDAVRLMDETGLEPEAIDECMKLGAGHPMGPLALLDFVG
                     VDVSIAIGESIGADVPQRLRDLAAEGRLGRKSGAGLYDYPPR"
     gene            1273..2448
                     /locus_tag="VF752_04475"
     CDS             1273..2448
                     /locus_tag="VF752_04475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX8854835.1"
                     /translation="MPMQNNGREVVIVEAVRTPIGRGHPEKGYYRDTHPNELLGKTYS
                     EVISRAGIDASEVEDVVTGCVQQYGEQMFNVGRNAWLQAGLPIETPATTVDRQCGSAQ
                     QAVNFAAALIASGVHDAAIGSGVEHMGHIPMGVGFKWVDEVGSPWPEELMAKYNLVPQ
                     GISAEMIADKWEIPRSELDEIGVRSHKLAAQATEEGRFEREIVPFQVNGDTYVTDQGI
                     RPDTNLETLANLKPAFKEDGKITAGNSSQISDGAAAVLLMERQKAEALGLKPRAVIVD
                     QTTVGVDPVIMLTGPIPATKKLLDRNKMTINDIDLFEVNEAFASVLAAWRRELEPDMD
                     RVNVNGGAIALGHPLGSTGARLITTLLHELERSDKEIGLVTMCCGGGLGTGTIIQRV"
     gene            2530..3981
                     /locus_tag="VF752_04480"
     CDS             2530..3981
                     /locus_tag="VF752_04480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8854836.1"
                     /translation="MAVRTNGQLFAGRYRFVKTLGRGGMASVVLAEDERLGRRVAIKR
                     LHADSPEDMRQRLQREARIGASLNHPNLVSVFDIETYDEGVLVVMEYVEGETLGEALR
                     DGPLSERRGLEVISAVASALDHVHEAGVVHRDVKPGNVLLGSNGAVKLVDLGIGTAVD
                     QTRITRTGTVLGTPAYMAPEQVEGGQVTRAVDIYALATVAFETLSGRKAREGRTPMEI
                     AHRVATEPPPNLAAVSPDAPPEAAALLKRAMSPDPSKRPRSAGALAEELEAAYARRRA
                     QRGRTRATRALPATARVASRPQRARPAWLPAAAVVALLALVGVVVGLLAAGGGDSGSG
                     SHNTADRGQSTAKHRSSNRNGSSGQGSTSTPATPVAPPADNGPALPEQAPPGKGDVEQ
                     ASALNDQGFAKLQQGDASGAIPLLQQSLSLFPKKTTDIRYAYALFNLGDAYLRAGQPE
                     KAIQVLEKRLKIPNQTSAVQAKLDEARAAAGKK"
     gene            4043..4726
                     /locus_tag="VF752_04485"
     CDS             4043..4726
                     /locus_tag="VF752_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX8854837.1"
                     /translation="MGTEETRRFHDRRHAGHLLAARLRHYAGRNDVIVLGLPRGGVPV
                     AYEVARELDAPLDVFLVRKLGVPGQEELALGAIASGGIVVINRGLVETVGLTREDIAG
                     IVARERRELERREREYRGDRPPPDLSGRVVILVDDGLATGSTMFAAVHAVRQENPARV
                     VVAVPVSDRDVCDRLGREADEAVCVFTPRPMAAVGFWYEDFSQTSDEEVRELLERARR
                     PAPARTRGG"
     gene            4723..5421
                     /locus_tag="VF752_04490"
     CDS             4723..5421
                     /locus_tag="VF752_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8854838.1"
                     /translation="MSGAAARHGTHADVAVSVRGAELAGLLAVPPHARGIVVFAHGSG
                     SGRFSPRNLAVATALEHAGLATLLLDLLTPEEEAEDVRTRRLRFDVRLLGEGVVGAID
                     SIAADAFVGDLPPSLSSLPVGCFGASTGAAAALIAAAVRSERVRAVVSRGGRPDLAGD
                     ALQRVSAPTLLIVGGRDTQVVELNRMAQRLLAGPSRVELVPGAGHLFEEPGALERVAE
                     LAIDWFLEHLARTR"
     gene            5542..6678
                     /locus_tag="VF752_04495"
     CDS             5542..6678
                     /locus_tag="VF752_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8854839.1"
                     /translation="MDLTLSPQEQSFRDQLRSWLDENNPGKEPAGDEAAFEFRRDWQK
                     KLHEAGYAGLSWPKEFGGRGATLVEQAIFNEEMARSRAPATANVLGLVMGGPVVIAHG
                     TEEQKKRWLEPILSAEEIWCQGFSEPESGSDLASLKTKAVKSNGEWIVTGQKVWTTFA
                     HESKWCMLLARTDQDAPKHKGLTYFILDMEQEGVQVRPLRQITGEAEFNELFFEEARI
                     PDENVIGRPGEGWMVGITTLMHERAGIAFGSAIAVKIALGELIALAKDRGVADDPVTR
                     QRIAQLWIETETLRLNAYRGLTHQMKHGAPGPEGSLPKWQWSDINQALTELAMDIRGE
                     EGPIADSSWTYRFLRARANSIEGGTTEILKNIIAERVLGLPKLR"
     gene            6686..7702
                     /locus_tag="VF752_04500"
     CDS             6686..7702
                     /locus_tag="VF752_04500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8854840.1"
                     /translation="MNFDFSDDQQAIKRTAREFLASRFKAEKVRELAEQGEYDDSLWT
                     EISELGWPGIFLDEGHGGMELGAVELVIVQEELGYALAPTPFLSNVAAALVLQHAGSD
                     EQKQRWLPGIASGESRGAVGVARNGEALLVPDADSADVIVLIDGDSATLHEGSSVERE
                     LRDTIDSTRRYATVRGNGGQPLPGDVGPALDRIEIAIAAENVGIAQRAMEMAVEYAKD
                     RKQFDRPIGAYQAVSHRCAQMLLETESARSAVYYAGWVADNEPETLPIAASMAKAYAS
                     DAGWRVTAASLQVHGGIGFTWEHDLQFFLKRARTNGTLFGSARQHRERVAELAGLTRE
                     AAAV"
     gene            7699..9069
                     /locus_tag="VF752_04505"
     CDS             7699..9069
                     /locus_tag="VF752_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8854841.1"
                     /translation="MTTRVAPELIDLLGEEAVLSEPPAIYGHDATEHEGLEGKPDLVV
                     VPRDTEQVRAAVKWCYANDVPIVPRGGGSGFSGGAVPVRGGVVISLERMTRVRSFDPG
                     LWRMEVEAGLRTADVRRLARENGLFYPVDPGAAEQSHIGGNIATNAGGPHAFKYGVTG
                     HWVTGLEAVVPPGEIVSVGGPVRKDVAGYDLRGLLIGSEGTLGIVTAAWLRLIPAPEA
                     ALPVVAAYPGIAEGCAAIEAVLASGVLPAAVEYLDENAVAASRGAFPEQLPSDARFVV
                     IAEADGSAEEAARVAGELREAMADGAVLVHEPSVRGDVEALWRWRDGVSIAVAAVRGG
                     KMSEDIAVPLDRLRDAIQGTLDIAGRHGLDACSWGHAGDGNLHSTFMIDSRNDDEVRR
                     AHDAVSELFELAAELGGTISGEHGMGWVKRGRLGLQWSDRAVELHREIKRMFDPKGLM
                     NPGKKE"
     gene            complement(9017..10231)
                     /locus_tag="VF752_04510"
     CDS             complement(9017..10231)
                     /locus_tag="VF752_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854842.1"
                     /translation="MATVVVSAYRVATKPEIGGHFWVYLQYAHALRRLGCDVVWLEKV
                     PNGALSRSPGTIPAFLSRMARYGLSGKVILYEPTRNGDLRFLGMERADAEAVLGRADL
                     LLNFHYAIDPSVLSRFRRTALVDIDPGLLQFWIAGGQLQVAPHDFYLTTGETVGTPGS
                     RIDDCGLPWMHIRPPVCLDLWPYKHDAACRRFTTVSSWLAREYLTEGDRVIYQNDKSV
                     SFLRFAELPSRASCELELALYLEGRVDAEDRELLERHGWRVRHALEVAGSPERYRAYI
                     QSSRGEFSCVKPSCVELQNAWVSDRSLCYLASGKPVVVQDTGASAFLPDGEGMFRFST
                     IDEAADALAAIDADYERHCRAAREIAEKFFDAETILAEVLELTETSPSRVNPSVTPSS
                     RGSSSPSGRTSA"
     gene            complement(10244..10912)
                     /locus_tag="VF752_04515"
     CDS             complement(10244..10912)
                     /locus_tag="VF752_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX8854843.1"
                     /translation="MPYLVVNADDFGLCSGVSRGIVDAHRHGIVTSTSLMTRGRRSTD
                     AARLAANCPRLSVGLHVVISNEHDVAASRAELEDQLVRFRELMGRGPTHLDSHHHVHL
                     QPKLAPAFRDAAQRCGVPLRACSEVRYCPSFYGQWAGDSHPEQVGVKGFMRVVDTEVA
                     DGITELACHPGYPDPELHSTYSSERALELQTLRDPRIRHFLQRREISLIGFEEVPRLL
                     ATPA"
     gene            complement(10913..13708)
                     /locus_tag="VF752_04520"
     CDS             complement(10913..13708)
                     /locus_tag="VF752_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8854844.1"
                     /translation="MAEGRKYSDTALYRRLAALARPYWAHLSVLFVLSLLATPLALLV
                     PVPLKIAVDSALGSQPLPGFVDALVPGAAPSQSSALAIAIVLLVVVAVLTQLQQLAVT
                     VLSSYTGEKLLIRLRGRMFGHVQRLSLSYHDTRGTADANYRIQYDATAIQWIAVTGLI
                     PVIEAVFMLIGMIYVTALIDWQLALVAMTIVPVLLVVTNAYRQRLRRRWRTAKELESS
                     AMSVVQEVLTGLRLVKAFGQEEREQDRYVRRAAEGTSARIRLAVIDGVFGLILGLTAA
                     AGSGIVLYVGVHRVQSNAITLGELLVVMGYLTQLYAPLQTVSRQAGKVQSSLASAERV
                     FVLLDQEPDVPERRRARRLKRAAGEIECRDVTFAYGGGRPALEGVSFHVPPGARVGIA
                     GTTGSGKSTLASLLMRFYDPTGGRVLLDGVDLRNYRLPDLRNQFALVLQDPILFSTTV
                     LENIAYARPGARTKEIVDAARAANAHDFIMRLADGYDTVLGERGVTLSGGERQRISLA
                     RAILKDAPILVLDEPTSSVDVRTESAIMEAMERLMSDRTTFMIAHRLGTLRSCDIRLQ
                     IEDGRVTSVKDRRDAAKAARARRRSGPAPRKVMDLARHTVTLPGEDARLVADEPLNKP
                     LKRSVRRLRFEAEGRTESVVVKRLTRRRAETTRMLADRWLPATGLEWACARVLAATDE
                     PGGRGVWLVYEDLQGDMLDCRSLDPTRVGPVVELIAELHARFCGHDLLAECRKYGEEL
                     GMDFFTSEVARCIRHLRSIKSAPRRLAADDAVLTDRLLERVERLYGERQERALTFEAL
                     AGRDTLLHGDLWTTNTLVVDGSTGPQPRLIDWDHTGVGPATYDISTFLYRFPGEHRPW
                     ILDRYREALAWRGWQLPDDRELNALFETSEYARYACCLAEAALAASQGKSWGREQMAE
                     IDTWFASMEPVLALEGS"
     gene            complement(13701..>15025)
                     /locus_tag="VF752_04525"
     CDS             complement(13701..>15025)
                     /locus_tag="VF752_04525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8854845.1"
                     /translation="FNEGDWAAVGGPNLAPSDGAVADCVANAPGGPVHVLLSDREAEH
                     IPGCNMAFRRSTLSEIGGFDPRFRVAGDDVDICWRLRARALKIGFNPAAVVWHHRRRS
                     VRAYLSQQTGYGRAEALLERKWPEKYNRARNVVWRGRLYRNGGTGELGGVRRWRVYYG
                     SQGSAPFQSIYPPASKGVAALALIPEWSLVIALLTALSALGATWRPLLIAAAPLAALA
                     LGVVLLHAIRGAAGADFRRTGASPLRTFGMRALTALLHVAQPMARLHGRLSQGLSPWR
                     CRAALRPRVPTPRSWAFWSTRWIDPQERLRAIERRIAIAGANVTRGGNFDHWDIEVQV
                     GMLVRERLLMAVEEHARGAQLIRLRASPHFSAAGLAVVAILYALAVPAAIDHAGIVAA
                     VLSALLLVLVGWGVWEWAVVAGTVADAVEQADESVTPIRRRKGNWSRG"
BASE COUNT         2411 a         5134 c         5260 g         2220 t
ORIGIN      
        1 acggcttgcc gaagccgaac tgccggtata ggtcgtgcgc gtcgtacgta tgcagcagcc
       61 agcggcgctt gctgtaggga ccgagcacca ccgtctcgcg gacgagctcc ttgccgagcc
      121 cgcggccctg atgttcgggg aggatgtaga cgtcggcgag gtacgcgaac gcgacgccgt
      181 ccgtgaccgt gcgcgagaag ccgacctggc ggccctcctt gtagatgccc accacccgct
      241 gcgcctcgcg gacgagtcgc tcgacagtct cgcgcgagcg cccgcgcgcc cagtaagact
      301 cgccgctcag gtagcggaag accgcgtcga tgtcgatgcg cgcggggtcg tcgtcgagct
      361 cgtagccgtc gcccagttcg cgcttcatcg cggcggatag tcgtagagcc cggcgccgga
      421 cttgcgtccg agccggccct ccgcggcaag gtcgcgcagg cgctgcggca cgtcggcgcc
      481 gatcgactcg ccgatcgcga tcgacacgtc tacgcccacg aagtcgagca gcgcaagcgg
      541 gcccatcggg tggccggcgc cgagcttcat gcactcgtcg atcgcctcag gctcgaggcc
      601 ggtctcgtcc atcaggcgaa ccgcgtcgaa caggtagggg aaaagcagcc ggttcacgac
      661 gaacccgggc gtgtcgggca cctccaccgg cgtcttgccg agctcgtcgc agaacgcgcg
      721 ggcgcgccgg cgcgtgtcct cgctcgccgc gttcgggaag gcgagctcga ccagcttcat
      781 cttctcgacg gggttgaaca cgtgcagccc gacgacgcgc tcgggcctcc cgctcgcgtc
      841 cgcgagctcg gagatcgaga gcgaggaggt ggtcgaggcg atcacggtgt cctcgtgcgc
      901 gagatcgtgg aggcgttgca gcatctcggc cttggcctcc gggtcctcga cgatcgcctc
      961 gacgatcatg gtggagccat cgagctcgcc gaggtcggtc accacgcgca cgccgtcgtc
     1021 aacacgcccc tgcgcacgat cggctgaatc gtcgctcctg gcccacagga cgacgtctcc
     1081 aaagggagcc gcggtgcgcg ccagaccagt cgcaatggcc ccgctgcccg ctatccccag
     1141 cctctcggaa agccttggtt gactcaccag tcaattcagt acagtacctg tagttggcgc
     1201 ggcagagcgt cgtcaccaag gcctcaggcc cgcgccgcct gagctcagaa acacgcttcc
     1261 ggagggacca gaatgccgat gcagaacaac ggccgcgagg tcgtcatcgt cgaggcggtt
     1321 aggactccga tcgggcgcgg tcatcccgag aagggctact acagggacac gcatccgaac
     1381 gagctgctcg gcaagacgta ctccgaggtg atctcgcgcg ccggcatcga cgcctccgag
     1441 gtcgaggacg tagtgaccgg ctgtgtccag cagtacggcg agcagatgtt caacgtgggc
     1501 cgcaacgcgt ggctccaggc cgggctgccg atcgagacgc ccgccacgac cgtcgaccgc
     1561 cagtgcggct cggcgcagca ggccgtgaac ttcgccgcag cgctgatcgc ttccggcgtg
     1621 cacgacgccg cgattggctc cggcgtcgag cacatgggcc acatcccgat gggcgtcggc
     1681 ttcaagtggg tcgacgaggt cggctcgccg tggccggagg agcttatggc gaagtacaac
     1741 ctcgtgcctc agggcatctc ggccgagatg atcgccgaca agtgggagat cccccgctcc
     1801 gagctcgacg agatcggcgt gcgctcgcac aagctggccg cgcaggccac cgaggagggc
     1861 cgcttcgagc gcgagatcgt gcccttccag gtgaacggcg acacttacgt gaccgaccag
     1921 ggcatccgcc cggacaccaa cctcgagacg ctcgcgaacc tcaagccggc cttcaaggag
     1981 gacggcaaga tcacggccgg caactcgtca cagatctccg acggcgccgc cgccgtgctg
     2041 ctgatggagc gccagaaggc cgaggcgctc ggcctcaagc cgcgcgcggt gatcgtcgac
     2101 cagacgaccg tcggcgttga tcccgtgatc atgctcacgg gcccgatccc ggccacgaag
     2161 aagctcctcg accggaacaa gatgacgatc aacgacatcg acctgttcga ggtgaacgag
     2221 gcgttcgcgt ccgtgctcgc cgcatggcgg cgcgagctcg agccggacat ggaccgggtg
     2281 aacgtgaatg gtggcgcgat cgcgctcggt cacccgctcg ggtcgacggg cgcccgcctg
     2341 atcaccacgc tgctgcacga gctcgagcgc tccgacaagg agatcggcct cgtcacgatg
     2401 tgctgcggcg ggggattggg cacgggcacc atcattcaga gggtctgatt cgcgaggggg
     2461 gcgccctgcg gggcgcccct ttctctacgc ggcgtttcac cgcttcgccg acgcgggtag
     2521 acggatcttg tggccgtaag gacgaacggt cagctgttcg cggggcgcta ccgcttcgta
     2581 aagacgctcg gcaggggagg gatggcctcg gtcgtcctcg ctgaggatga gcggcttggt
     2641 cgccgcgtcg ccatcaagcg cctgcacgcg gacagccccg aggacatgcg ccagcggctc
     2701 cagcgggagg cgaggatcgg cgcatccctg aaccacccca acctcgtctc ggtgttcgac
     2761 atcgagacct acgacgaagg cgtgctcgtg gtcatggagt acgtggaggg cgagacgctc
     2821 ggcgaggcgc tgcgcgacgg gccgctgtca gagcgccgcg ggctcgaggt gatctcggcg
     2881 gtcgcgtcgg ctctcgatca cgtgcacgag gccggagtgg tgcaccgtga cgtgaagccc
     2941 ggcaacgtac tcctcggctc gaacggggcg gtgaagctgg tggacctcgg aattggcacg
     3001 gccgtggacc agacacggat cacccgcacg ggcaccgtcc tcggcacgcc cgcgtacatg
     3061 gcccccgagc aagtggaagg cggtcaggtg acgcgggcgg tcgacatcta cgcgctcgcg
     3121 accgtcgcgt tcgagacgct gagcgggcgc aaggctcgcg aagggcggac gccaatggag
     3181 atcgcccatc gcgtggcaac cgagccgccg ccgaatctcg ccgcggtgtc gcccgacgcc
     3241 ccacccgagg ccgccgccct cttgaagcgc gcgatgtcgc cggatccgtc caagcgaccg
     3301 cggtctgcgg gcgccctcgc cgaggagctc gaggccgcgt acgcacggcg gcgcgctcag
     3361 cgcggacgca cgcgtgccac gcgggcgctg ccggccacgg ctcgggttgc gtcccggccg
     3421 cagcgtgcac ggcccgcctg gctgccggcg gcagcggtcg tcgcgctgct cgcactcgtc
     3481 ggcgttgtcg tcggcttgct cgctgccggt ggaggcgact caggcagcgg ctcgcacaac
     3541 accgcggatc gcggccagag caccgcgaag caccgaagct cgaaccgcaa cggatcgtcc
     3601 ggccagggct cgacgtcgac tcccgccacc ccggtggcgc cgccggcaga caacggcccc
     3661 gccctgccgg agcaggctcc gcccgggaag ggcgacgtcg agcaggcgtc ggcgctgaat
     3721 gaccagggct tcgcgaagct ccagcagggt gacgcgtcgg gggcgatccc gctgctccag
     3781 caatcgctgt cgctcttccc gaagaagacg acagacatcc gctacgcata cgcgctcttc
     3841 aacctcggcg acgcgtacct gcgcgcgggg cagcccgaga aggcgattca ggttctcgag
     3901 aaacgcctga agatcccgaa ccagacgagc gccgtgcagg cgaagctcga cgaggcccgg
     3961 gcggctgccg gcaagaagta acccgagcac gttgcgcgct tgcgcggcac gcgcgcggcc
     4021 ctaccgtttg cggtgagcgc ccatggggac ggaggagaca cggcggttcc atgaccgacg
     4081 ccacgccggc cacctgctcg cagcgcgctt gcgccactac gccggccgca acgacgtgat
     4141 cgtcctcggc ctgccgcgcg ggggcgtccc ggtggcatac gaggtggcgc gcgagctgga
     4201 cgccccgctc gacgtgttcc tggtgcgcaa gctcggcgtg ccgggccagg aggagctcgc
     4261 gctcggcgcc atcgccagcg gcgggatagt cgttatcaac cgcgggctgg ttgaaacggt
     4321 cgggctcacc agggaggaca tcgcggggat cgtcgcgcgg gagcggcgcg agctcgagcg
     4381 gcgcgagcgt gagtaccggg gcgaccggcc cccgcccgac ctgagcggcc gggtggtgat
     4441 cctggtcgac gacgggcttg cgacgggctc gacgatgttt gcggccgttc atgcggtccg
     4501 gcaagagaac cctgcgcgcg tggtcgtggc cgtgccggtg tccgatcgag acgtctgcga
     4561 ccggctcggc cgggaggcgg acgaggcggt ctgcgtgttc acgccgcggc cgatggccgc
     4621 tgtcgggttc tggtacgagg acttttcgca aacgagcgac gaggaggtac gtgagctcct
     4681 cgagcgcgcc cgccggcccg cgccagcgcg cacgaggggc ggatgagcgg cgcggccgcg
     4741 cgacatggca cgcacgccga cgtcgccgtc tccgtccgcg gcgccgagct cgccggtctg
     4801 ctcgccgtac caccgcacgc gcgcgggatc gtcgtcttcg cgcacgggag cgggagcggg
     4861 cgattcagcc cacgcaacct cgcggtggcg accgccctcg agcacgcggg tctcgcaacg
     4921 ctgctgctcg atctgctcac gcccgaggag gaagcggagg acgttcggac cagacgcctg
     4981 cgattcgacg ttcgtctgct gggcgagggc gtggtggggg cgatcgactc gatcgctgcg
     5041 gacgcgttcg tcggcgatct gccgccgtct ctctcatcgc tgcccgtagg ttgcttcggc
     5101 gcgagcaccg gcgcggcggc ggcgctcatc gccgcggcag tgaggtccga acgcgtgcgg
     5161 gcggtcgtct ctcgcggcgg gcgtcccgac ctcgccgggg atgcgctcca gcgggtcagc
     5221 gcgcccacgc tcctcatcgt cgggggacgt gacacccagg tggtcgagct caaccggatg
     5281 gcacagcggc tgctcgccgg cccctcgcgc gtcgagctcg tgcccggggc ggggcatctg
     5341 ttcgaggagc cgggcgcgct cgagcgcgtc gccgaactgg cgatcgactg gttcctcgag
     5401 catctcgcgc gcacgcgctg acgcgtcgcc gatcagtcgc ggtgcgcgat ctcggatgcc
     5461 cgtgcgcggt ccacgacctg agcaccctag ccagcgagtt gcaaacgctc gtgtctccca
     5521 cccggtagga tcagcgccgg tttggacctg acgctttcgc cccaggagca atccttccgc
     5581 gatcagctcc gctcgtggct cgacgagaac aacccgggca aggagccggc cggcgacgag
     5641 gccgcgttcg agttccgcag ggactggcag aagaagcttc acgaggccgg ctacgcgggc
     5701 ctctcgtggc cgaaggaatt cggcggccgg ggcgcgacgc tggtcgagca ggcgatcttc
     5761 aacgaggaga tggctcgctc gcgcgcgccg gcgacggcga acgtgttggg cctcgtgatg
     5821 ggcgggccgg tggtgatcgc gcacggcacc gaggagcaga agaagcgctg gctcgagccg
     5881 atcctgtccg ccgaggagat ctggtgccag ggcttctccg agccggagtc gggctcggac
     5941 ctcgcctcgc tcaagaccaa ggcggtcaag tcgaatggcg agtggatcgt gaccggccag
     6001 aaggtctgga ccacgttcgc tcacgagtcg aagtggtgca tgctgctcgc gcgcaccgac
     6061 caggacgcgc ccaagcacaa gggcctgacc tacttcatcc tcgacatgga gcaggagggc
     6121 gtgcaggtgc ggccgctccg ccagatcacg ggcgaggccg agttcaacga gctcttcttc
     6181 gaggaagcgc ggatacccga cgagaacgtg atcgggcgcc cgggcgaggg ctggatggtg
     6241 ggcatcacca cgctcatgca cgagcgggcc gggattgcgt tcgggtctgc gatcgcggta
     6301 aagatcgcgc tcggcgagct gatcgcgctg gccaaggatc gcggcgtggc ggacgacccg
     6361 gtcacccgcc agcgcatcgc ccagctctgg atcgagaccg agaccctccg gctgaacgcg
     6421 taccgcggcc tgacgcacca gatgaagcac ggcgcgcccg gacccgaagg ctcgttaccg
     6481 aagtggcagt ggtcggacat aaaccaggcg cttaccgagc tcgcgatgga catccgcggc
     6541 gaggaggggc cgatcgccga ctcgagctgg acctaccggt tcctccgtgc ccgcgcgaac
     6601 tcgatcgagg gcgggaccac agagatactc aagaacatca tcgccgagcg cgtgctcggg
     6661 ctcccgaagc ttcgctagga acggcatgaa cttcgacttc tccgacgacc agcaggcgat
     6721 caagcgcacc gcccgcgagt tcctggcgtc tcgcttcaag gcggagaagg tgcgcgagct
     6781 cgccgagcag ggcgagtacg acgactccct ctggacggag atctccgagc tcggctggcc
     6841 ggggatcttc ctcgacgagg gccacggcgg catggagctc ggcgccgtgg agctcgtgat
     6901 cgttcaggag gaactcgggt atgcgctcgc tcccacgccc ttcctctcga acgtcgccgc
     6961 cgcgctcgtg ctccagcacg ccggcagcga cgagcagaag cagcgctggc tgcccggaat
     7021 cgcgtccggt gagtcgcgcg gcgctgtggg cgtggctcgc aacggcgagg cgctgctcgt
     7081 gccggacgcc gactccgcgg atgtgatcgt gctgatcgac ggcgactcgg ccaccctcca
     7141 cgagggcagc tctgtcgagc gcgagctgcg cgacacgatc gactcgaccc gccgctacgc
     7201 caccgttcgc ggcaacggcg gccagccgct gccgggcgat gtgggcccgg cgctcgaccg
     7261 catcgagatc gcgatcgctg ccgagaacgt gggcattgcc cagcgcgcca tggagatggc
     7321 cgtcgagtac gccaaggacc gcaagcagtt cgaccgcccg ataggcgcct accaggccgt
     7381 gtcgcaccgc tgcgcgcaga tgctgctcga gacggagagt gcgcgctccg ccgtctatta
     7441 cgcgggctgg gtggccgaca acgagccgga gacgctgccc atcgcggcct cgatggcgaa
     7501 ggcgtatgca tccgacgccg gctggcgcgt gacggccgct tcgctgcagg tgcacggcgg
     7561 gatcggcttc acctgggagc acgacctcca gttcttcctc aagcgcgcac gcacgaacgg
     7621 cacgctcttc ggcagcgccc gccagcaccg cgaacgcgtg gccgaactcg ccggtctgac
     7681 tcgcgaggcc gcggccgtgt gaccacgcgg gtcgcgcctg agctgatcga tctcctcggt
     7741 gaggaggccg tgctcagcga gccgcccgcg atctacggcc acgatgcaac cgagcacgag
     7801 gggctcgagg gcaagcctga cctggtcgtg gttccacgcg acaccgagca ggtacgcgcg
     7861 gcggtgaagt ggtgctacgc aaacgacgtg ccgatcgtgc cgcgcggcgg cggcagtggc
     7921 ttctcgggcg gtgccgtgcc ggtccgcggc ggcgtggtga tctcgctcga acgcatgacc
     7981 cgcgtgcgca gcttcgaccc cggcctgtgg cgaatggagg tcgaggccgg cctccgcacc
     8041 gcggacgtcc ggcgcctcgc tcgcgagaac ggtctcttct acccggtcga ccccggcgcc
     8101 gccgagcagt cgcacatcgg cggcaacatc gcgacgaacg ccggcggccc gcacgccttc
     8161 aagtacggcg tcacgggcca ttgggtgacg ggcctcgagg cggtggtacc gccgggggag
     8221 atcgtgtcgg tcggcgggcc ggtgcggaag gacgtcgccg gctatgacct gcgcggcctg
     8281 ctgatcggct cggagggcac gctcgggatc gtgacggcgg cttggctgcg gctcatcccc
     8341 gccccggagg ctgcgctgcc ggtggtcgcc gcgtatcccg gaatcgccga gggctgcgcc
     8401 gcgatcgagg cggtgctggc atcgggcgtc ctgcccgcgg cggtcgagta cctcgacgag
     8461 aacgcggtcg ccgcatcgcg cggcgcgttc ccggagcagc tgccgagtga cgcgcgcttc
     8521 gtggtgatcg ccgaggccga cgggtctgcc gaggaggctg cgcgggtcgc cggcgagctg
     8581 cgagaggcga tggcggacgg cgccgtgctc gtgcacgagc cgagcgtgcg cggggacgtc
     8641 gaggcgctct ggcgctggcg cgacggcgtc tcgatcgcgg tggccgctgt gcgtggcggg
     8701 aagatgagcg aggacatcgc ggtgccgctc gaccgcttgc gcgacgcgat acagggcacg
     8761 ctcgacatcg ccggccggca cggcctcgac gcgtgcagct ggggccacgc cggcgacggc
     8821 aacctgcact ccacgttcat gatcgactcg cgcaacgacg acgaggtgcg gcgagcgcac
     8881 gacgctgtga gcgagctgtt cgagctcgcc gcggagctcg gcggcacgat ctcgggcgaa
     8941 catggcatgg gctgggtgaa gcgcggccgc ctcggcctcc agtggtccga ccgcgcggtc
     9001 gagctgcacc gcgagatcaa gcggatgttc gacccgaagg gcttgatgaa ccccgggaag
     9061 aaggagtaac cgacgggttc actcggcttg gcgaggtctc cgtgagctcc aggacttcag
     9121 ccaggatcgt ctcagcgtcg aagaatttct ccgcgatctc tcgcgctgct cggcagtgcc
     9181 gttcgtaatc ggcgtcgatc gcggccagcg cgtcggcggc ctcgtcgata gtggagaaac
     9241 gaaacatccc ctcgccgtcc ggcagaaagg cactcgcgcc ggtgtcctgc accaccaccg
     9301 gcttgccaga ggccagatag cagaggctgc gatcgctaac ccacgcgttc tgaagctcga
     9361 cgcaggaggg cttcacgcag ctgaactcac cacgggacga ctggatgtag gcgcgatatc
     9421 tctcagggct accggccacc tcgagtgcgt ggcggacgcg ccatccatga cgctcgagca
     9481 gctcgcggtc ttcggcgtcg acccgtccct cgaggtaaag ggcgagctcg agttcgcaag
     9541 aggctctcga ggggagctcg gcaaatcgca ggaacgagac actcttgtcg ttctggtaga
     9601 tgacgcggtc tccctcggtc aggtactccc tcgccagcca actcgacacc gtcgtgaacc
     9661 gccggcacgc cgcgtcgtgc ttgtagggcc agagatcgag gcagacgggt ggtcggatgt
     9721 gcatccacgg gaggccgcaa tcgtcgatgc gactgccggg cgtcccgacg gtctcgcctg
     9781 tcgtcaaata gaaatcgtgc ggagcgactt gcagctgccc accggcgatc caaaactgca
     9841 gcagcccagg gtcgatgtcc acgagagcgg tgcgccggaa gcggctcagg acgctcgggt
     9901 cgatggcgta gtggaagttg aggagcaggt cggcgcgccc gagtactgcc tcggcgtctg
     9961 cccgttccat gccaaggaac cggagatctc cgttgcgcgt cggctcgtag aggatcacct
    10021 tcccgctcag tccgtagcgt gccatgcggc tcaggaaggc cgggatggtt cccggacttc
    10081 ggctcagggc gccattgggc accttctcga gccagacgac gtcacatccg agccgccgca
    10141 gcgcgtgtgc gtactgcagg tagacccaga agtggccgcc gatctctggc ttggtagcca
    10201 ccctgtacgc agagacgacg accgtggcca tgccagaccg ctctcacgcc ggcgtggcga
    10261 ggaggcgggg cacctcctcg aagccgatca gtgagatctc gcgccgctga aggaagtggc
    10321 ggatccgggg atcgcgcagc gtctgcagct cgagcgcgcg ttcggaggag tacgtggaat
    10381 gcagctcggg gtcggggtag ccggggtgac aggcgagctc cgtgatgcca tcagccacct
    10441 cggtgtcgac cactcgcatg aagcccttca caccgacttg ctcgggatgg ctgtcgccag
    10501 cccattgtcc gtagaagctt gggcagtacc gcacctccga gcacgcccgc agtggaactc
    10561 cgcatcgctg tgccgcgtcc cggaacgcgg gcgcgagctt cggctgaaga tggacgtggt
    10621 ggtgcgagtc gaggtgggtg ggcccgcgac ccatcagctc cctgaagcgc acaagctgat
    10681 cctcgagctc ggcacggctc gccgcgacgt cgtgctcgtt cgagatcacg acgtggagcc
    10741 ccacgctgag gcgcgggcag ttggcggcca gccgggctgc atccgtgcta cggcgacccc
    10801 tcgtcatcag gctcgtgctc gtcacgatcc cgtgccggtg cgcgtcgacg atccccctac
    10861 tcacgccgga gcagaggccg aagtcgtcgg cattcacgac cagatacggc attcaggaac
    10921 cttccagcgc aaggacgggt tccatggacg caaaccaagt gtctatctcg gccatctgct
    10981 cgcgccccca gctcttcccc tgcgaagccg ccagggcagc ctcggccagg cagcacgcat
    11041 agcgcgcgta ctccgaggtc tcaaacagcg cgttcagttc gcggtcgtcc ggcagttgcc
    11101 acccccgcca ggcgagggcc tcgcgatagc ggtcgaggat ccaaggccgg tgctcgccag
    11161 gaaagcggta caggaacgtc gagatgtcgt acgtagccgg ccccacgccc gtgtggtccc
    11221 agtcgatcag cctcggctgc ggtccggtgc tcccgtcaac cacgagcgtg ttggtggtcc
    11281 acagatcccc gtgcagcagt gtgtcgcgac ccgcgagcgc ctcgaacgtg agcgcgcgct
    11341 cttgacgctc gccgtagaga cgctccactc gctcgagcag gcggtctgtg agcacggcgt
    11401 cgtcggccgc gagccgcctg ggtgcggact tgatcgaccg gagatggcga atgcaccgcg
    11461 ctacctctga agtgaagaag tccatcccga gttcctcgcc gtacttgcgg cactcggcga
    11521 gcaggtcatg tccgcagaag cgcgcgtgca gctcggcgat cagctccacc acgggcccga
    11581 cgcgtgtcgg atcgagggag cggcagtcca gcatgtcgcc ctgcaggtcc tcgtagacaa
    11641 gccagacacc tcttcctccg ggttcatcag tcgccgccaa cacgcgcgcg caggcccact
    11701 cgagcccggt ggcggggagc catcggtcgg ccaacatccg cgtcgtttcg gcgcgccggc
    11761 gagtgagccg tttcaccacg acggactcgg tacggccttc ggcctcgaac cgcaggcgtc
    11821 gaaccgaccg cttgagcggc ttgttgagcg gctcgtcggc cacgagcctt gcgtcttcgc
    11881 ccgggagcgt gacggtatgc cgtgcgagat ccatgacctt ccgcggtgcc ggaccactcc
    11941 ggcgtcgcgc gcgagccgcc ttcgccgcgt ccctgcggtc tttcacactc gtcacccgtc
    12001 cgtcttcgat ctggagccgg atgtcgcagc tgcggagcgt tccgagtcgg tgggcgatca
    12061 tgaacgtcgt ccggtccgac atgagcctct ccatcgcctc catgatcgcc gactcggtcc
    12121 ggacgtcgac cgagctcgtc ggctcgtcga gcaccaagat cggcgcgtcc ttcaggatcg
    12181 cgcgcgcgag cgagatgcgc tggcgctcgc caccagacag cgtcacgccg cgctcgccta
    12241 gcaccgtgtc gtacccgtcc gccaggcgca tgatgaagtc gtgtgcgtta gccgcccgcg
    12301 cggcgtcgac gatctccttc gtgcgcgcgc ccggtcgcgc gtacgcgatg ttctcgagga
    12361 cggtggtgga gaagaggatc gggtcttgta ggacgagcgc gaactggttg cgtagatccg
    12421 gtaggcggta gttgcgcagg tccacgccgt cgagtaggac gcggccgccg gtggggtcat
    12481 agaaccgcat cagcaggctg gcgagcgtgc tcttgccgga tccggtcgtg ccggcgattc
    12541 caacgcgcgc gccgggaggc acgtggaacg agacgccctc cagcgcgggt cggccaccgc
    12601 cgtaggcgaa cgtcacgtcc cgacactcga tctcaccggc agcgcgcttc agacggcgcg
    12661 cgcggcggcg ctccggcaca tctggctcct ggtcgagcag cacgaagacg cgttcggcgc
    12721 tggcgagtga ggactggacc ttgccggcct ggcggctcac ggtctgaagt ggcgcataga
    12781 gctgggttag gtagcccatc accacgagga gctcgcccag tgtgatcgcg ttcgactgga
    12841 cgcgatgcac gcccacgtac agcacgatgc ccgagcccgc ggctgccgtg agacccagga
    12901 tcagtccgaa aacgccgtcg attacggcga gccgtattcg tgcggaggtt ccctccgctg
    12961 ctctgcgcac gtaccggtcc tgctcgcgct cctcctgacc gaacgccttc accaggcgca
    13021 ggccggtgag cacctcctgc acgaccgaca tggcggagct ctcgagctcc ttcgccgtgc
    13081 gccagcgccg gcgcaagcgt tgccggtagg cgttggtcac cacgagcagc accggcacga
    13141 tcgtcatcgc gacgagcgcc agctgccagt cgatcagcgc ggtcacatag atcatcccga
    13201 tcaacatgaa gaccgcctcg atcaccggga tcagaccggt cacggcgatc cactgaatgg
    13261 cggtcgcgtc gtattggatc cggtaattgg cgtccgcggt gccacgcgtg tcgtggtacg
    13321 acaacgagag ccgctggacg tggccgaaca tacggccgcg caggcgaatg agcagcttct
    13381 cgcccgtgta ggagctgagc acggtcaccg cgagctgctg aagctgtgtc agcacggcga
    13441 ccaccacgag cagcacgatc gcgatcgcca acgcgctgct ctgcgacggg gccgcgccgg
    13501 gcacgagtgc gtccacgaag cccggaagcg gttgcgaccc gagcgcgctg tcgacggcga
    13561 tcttcagtgg caccgggacg aggagcgcca gcggggtggc gagcaggctc aggacgaaca
    13621 gcaccgagag gtgcgcccaa tacggccgcg ccagtgcggc caggcgccgg tagagcgcgg
    13681 tgtccgagta ctttcgccct tcagccacgg ctccaatttc ccttgcgccg gcggatcggc
    13741 gtaacgcttt catccgcctg ctctacggcg tcagcgactg tgccggcgac cactgcccac
    13801 tcccagacgc cccagccgac cagcaccagg agcagagcgg aaagcacggc ggcgacgatc
    13861 ccggcgtggt ctatcgccgc tggcaccgca agcgcgtaga gaatcgcgac caccgccaag
    13921 cccgcagccg agaagtgcgg gctggcgcgc agccgtatca gctgtgctcc gcgtgcgtgc
    13981 tcctccacgg ccatcagcag ccgctcgcgt accaacatgc ccacctggac ctcgatatcc
    14041 cagtggtcga agttgccgcc gcgcgtgaca ttcgcgccgg caatcgcgat tcggcgctcg
    14101 atcgcccgaa gcctctcttg cggatcgatc cagcgcgtgc tccagaacgc ccagctgcgc
    14161 ggcgtcggga cgcgcggccg caacgcagcc cggcatcgcc acggcgagag tccctggctc
    14221 aggcgtccat gcaggcgggc catcggctgg gcgacgtgga gcagcgcggt cagcgctcgc
    14281 attccaaacg tgcgcagcgg cgaggctcct gttcggcgga aatccgcgcc cgctgcgccg
    14341 cgtatggcgt gcaataggac cacgccgagg gccagcgccg cgagcggcgc ggcggcgatc
    14401 agaagcggtc gccacgtggc gccgagggcg ctcagggcgg tgagtagagc gatcaccaac
    14461 gaccactcgg gaatgagcgc gagcgctgcg acgcccttcg atgccggggg gtagatcgac
    14521 tggaacgggg cgctcccctg gctcccgtaa tacacgcgcc agcgccgcac gccaccgagt
    14581 tcgccagtgc cgccgttccg atacagccgc ccgcgccaga cgacgttccg agcgcggttg
    14641 tacttctcgg gccacttgcg ctcgagaagc gcctccgccc ggccatagcc tgtctgctgc
    14701 gagaggtagg cgcgcacgga gcggcggcgg tgatgccaca cgacggccgc cgggttgaat
    14761 ccgatcttga gcgcacgtgc tcgcagccgc cagcagatgt ccacgtcgtc ccccgccaca
    14821 cggaaccgcg ggtcgaagcc gccgatctca ctgagcgtcg agcgacggaa tgccatgttg
    14881 cagccgggga tgtgctcagc ctcgcgatcc gacagcaaca cgtggacggg gccgccgggc
    14941 gcgttagcca cgcagtcggc gaccgcgccg tcactcgggg cgaggttcgg gcctccgact
    15001 gctgcccagt cgccttcgtt gaacg
//
LOCUS       DASVXS010000077        17674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_17758, whole genome shotgun sequence.
ACCESSION   DASVXS010000077 DASVXS010000000
VERSION     DASVXS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17674
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17758"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VF752_04530"
     CDS             <1..1204
                     /locus_tag="VF752_04530"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8854846.1"
                     /translation="AAKIGCPVIGINDSGGARIQEGVVSLGAYGDVFVRNVKCSGVIP
                     QISLIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPDVIKTVTGEEPTFEELGGAMT
                     HNTKSGVAHFAADDEEQCLEDVRYLMSFLPQNNLETAPRVDSGDDPERMDLELDTIVP
                     DNPNKPYDMRDVIHRVVDNGEFFEVHEHYARNIVVGLSRLNGYPVGIVGNQPAQLAGV
                     LDIDASVKGARFVRFCDAFNIPLITFTDVPGFLPGTTQEWGGIIRHGAKLLYAFTEAT
                     VPKITVVTRKAYGGAYDVMNSKHMLADFNFAWPTAEVAVMGPEGAVNIIFRRDISSSP
                     TPDERRQKLISDYKARFANPYSAAERGYIDDVLIPHETRPRLCQALDMLQTKRVERPK
                     RKHGNIPL"
     gene            1320..2546
                     /locus_tag="VF752_04535"
     CDS             1320..2546
                     /locus_tag="VF752_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854847.1"
                     /translation="MRRCVAALFGVAIGLALLPAATSAAPPGMRDVLVVGNNWEGTAD
                     VVDARTFERLTRLNIIPDKAERMAEIQTDPAALGFFTGINLLIGEGHDQYVDDAFTSH
                     DGRYLYVSRPSFKDVVAFDLSTRQIVWRFQVEGYRADHMAISPDGARLLVSASTANKV
                     HEIDTATGEEIGQFPSGDSPHENNFSRDGQLIYHASIGRVYTPLDDPAFDATKGERYF
                     QVVDANTLQILKRVDMGKKLAEAGYPNMSSAVRPMALAPDEKRVYLQISFLHGFVEYD
                     LEHDKVLRVANLPVSEEAQKLRRDEYVLDSAHHGLAMNPEGTKLCVAGTMSDYAAIVS
                     RRTFAYQIAAHGEKPYWATNNGDGRYCFVSFSGDDRVSVISYAKQKEVASIPVGDHPQ
                     RMRMGVMREEYLQPPA"
     gene            complement(2543..3487)
                     /locus_tag="VF752_04540"
     CDS             complement(2543..3487)
                     /locus_tag="VF752_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX8854848.1"
                     /translation="MPEIPVFDYDSVLAAVSPAEAIDAVRNGFVQFASGEWAMPPKLY
                     LDVPNGDFRAMPAKGGGLAILKWVTSFPGNPASGVPVVMGVVCVSSSDDGEPLGLVDA
                     RAVTALRTGAVAAIAAQELAREDARNVGIIGCGVYGAWAARCLAAAEYGPGVCFDPDP
                     DAAGALAGELGWEAGSREEALEQDVVTCVTPGHEPVVHAPELRPGVHLNMLGADGPGK
                     AEAEVEAVVRCRLFCDEWAQASHGGELTGAVAAGVVDRERVTELGDVLTGRAPGRESA
                     EEMTLFDSTGLAIQDLAICQAVMHAHRQGAARAQTVRL"
     gene            3514..3783
                     /locus_tag="VF752_04545"
     CDS             3514..3783
                     /locus_tag="VF752_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854849.1"
                     /translation="MSRNGKHAPARPRIELSYGSASPEEAAAITAAIEQFLRDTAPAP
                     ATHSRPIDRWQRAALFEGAGLAGDAPSPWGDDQPWGHATVPPHGH"
     gene            3773..4141
                     /locus_tag="VF752_04550"
     CDS             3773..4141
                     /locus_tag="VF752_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8854850.1"
                     /translation="MATDEQQTPTPATELPPDEVDTTSVQIVDVRTDEERAAAHIPGD
                     VYIPLERLQEDAGTLDRERTVVFYCRGGDRSRAAAEAFRASGWDAYSVAGGLVAWAER
                     GLPVEPEGAQVVQRSQLPPY"
     gene            4251..6068
                     /locus_tag="VF752_04555"
     CDS             4251..6068
                     /locus_tag="VF752_04555"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX8854851.1"
                     /translation="MEPTTVKAPGRKGTLENPEVYENVPGHVIPILEREFDDFDNEAE
                     KFLAGDTPEDQFIGFRLKQGVYGQRQADVQMVRVKLPMGGITPEQMETFADVIEEYAP
                     LRKGHITTRQNIQIHHVPLPDAAKLIRRISDAGLSSREGCGNTVRNVTGDPWAGVSEG
                     ELFDITPYAGAYVRYFVRHPTTQLMPRKVKTAFTATDEDRAITGIHDIAFIPRIRDGV
                     RGVEVRVGGGTSIMPRIAPTLYEFVELDNGDYLKVAEAVFRIFDRQEWLRVNRARARI
                     KVLIDKIGIDAFREQVEEELQGDWLAERNFDPTPLLLIHDEEANAPARGLAYAQPNGD
                     LSEFERFSASNVQAQRQEGFSSVQVRVPRGDLSPEQLRGLAQIMRDYSGGYARTTVHQ
                     NFLLRWVRDESVYEVWDALGKLGLNAAGADEIVDVVSCPGTDSCKLGITSSMGLNRAV
                     QERVASMQIDDPLTKRIHIKMSGCPNGCSQHHIANIGFYGASIKVGDKTMPAYVAHIG
                     GQYEGGEVVYGNRLKVRLPAKRVPDAVERWLRWYEEDRNDGEEFNAFAERVGTGEFEA
                     RVKDLAMPVEFNLENLGHFIDWNRRDPYKVERGEGECAV"
     gene            6073..6729
                     /locus_tag="VF752_04560"
     CDS             6073..6729
                     /locus_tag="VF752_04560"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX8854852.1"
                     /translation="MAIAATFDVEHLDAEDVLAHVVERFHPRLSVACSFQKEASVIMD
                     MLLKIEPEARFFTLDTGVLFPETYATWRKAEERYGIKVDVYQGMSLARQADLHGDELW
                     ARDPDACCGIRKVAPLDEALADVDCWISGLRRDQSPTRANTPKLHWDEKHGLHKANPL
                     ADWSEKDVWTYIAEHDVPYNPLHDQGYPSIGCTYCTLAGDGRDGRWAGTGKTECGLHG
                     "
     gene            6746..7330
                     /gene="cysC"
                     /locus_tag="VF752_04565"
     CDS             6746..7330
                     /gene="cysC"
                     /locus_tag="VF752_04565"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX8854853.1"
                     /translation="MSDGYTLWFTGLSGAGKSTIAGIVESELRLRGQKVEVLDGDVVR
                     TNLSKGLGFSKEDRDTNIRRIAFVADLLSRNDVAAITAAISPYRETRDEARALHGERF
                     IEVYVKASVDECARRDVKGLYEKAFAGEIKEFTGVSDPYEEPLAAEIVVDTEAETPEE
                     SAAKVLAYIDARDRSNGNGHVARKQEEPAWQASN"
     gene            7312..8385
                     /gene="sat"
                     /locus_tag="VF752_04570"
     CDS             7312..8385
                     /gene="sat"
                     /locus_tag="VF752_04570"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX8854854.1"
                     /translation="MASIELNARQTADFELLANGGFAPLTGFQGSADWQSVIERMRLA
                     DGQPWPIPVTLASESGEVGETLELIGHEGTRLGTITIEEVYDRDLQREAELVYKTTED
                     AHPGVAALYREPARTIAGPISAEQLPSHIDAVQPYLLTPAEAKAAFAERGWKTVVAFQ
                     TRNPIHRAHEYITKGALELVDGLFIHPIVGETKSDDIPADTRMRCYEVLIENYYPRDR
                     AVLGVNPAAMRYAGPREAIFHALVRRNYGCTHFIVGRDHAGVGSYYGTYDAQEIFDEF
                     EPGELGIEPMKFEHAFFCKVTKTMATAKTSPSTPEQRVHLSGTAVRELLGRGERPPEE
                     FSRPEVADVLIEAYAAEGERAAA"
     gene            complement(8513..8983)
                     /gene="greA"
                     /locus_tag="VF752_04575"
     CDS             complement(8513..8983)
                     /gene="greA"
                     /locus_tag="VF752_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8854855.1"
                     /translation="MTEKLMTQAELDDLRGEIDRLEGEGRRQIAERIKTAREWGDLKE
                     NAEYHAAKEEQAHLETRILRLRDRLQNASVVEEGAAAAAGVAGLGATVEFVDETSGAS
                     QRFRLTSAGQARPGDGTLSFDSPVGRALAGHRAGETVVVHTPRGDRRLCITSIS"
     gene            complement(9037..9996)
                     /locus_tag="VF752_04580"
     CDS             complement(9037..9996)
                     /locus_tag="VF752_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEX8854856.1"
                     /translation="MGIEVFGISKSFGDFAALTDVSLNVPSGSLTALLGPSGSGKSTL
                     LRVIAGLELPDEGSVRIGGEDVTDAPAQRRGVGFVFQHYAAFKHMTVRDNVAFGLKIR
                     KLPKNAIEARVRELLALVHLGGWADRYPAQLSGGQRQRMALARALAVQPDVLLLDEPF
                     GALDARVRSELREWLRRLHDEIHVTTVFVTHDQQEAMEVAEQIAVMQDGRIEQTGSPA
                     EVYDRPASDFVMGFVGPVTKLDGSAVRPHDLELSREPEPGASEAVVERVVALGFEVRV
                     ELSQPGNGQLNAQITRAQAAELDPSPGETLYVRAREPDYASLA"
     gene            complement(9996..10790)
                     /gene="cysW"
                     /locus_tag="VF752_04585"
     CDS             complement(9996..10790)
                     /gene="cysW"
                     /locus_tag="VF752_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX8854857.1"
                     /translation="MRARFGLRSLALGYLFVLLVVPVGMVFYRAFEHGFAAAWDAVTT
                     PDALHALQLTLLMVVVAVPLNTIFGIAAALLIVRKRFRGKAVVEALIDVPFAVSPVVV
                     GLALILVYGRTGWLGDWLSQNGIRVIFSTPGMILATIFVSLPFVVREVAPVLREIGTE
                     QEEAARTLGASPWQTFSRITLPAIRWGVGYGVVLTTARAVGEFGAVSVVSGRLAGQTE
                     TLTLFVQDHFERFDLAGAYAASVVLAAIALSTVAVMTLLQRRRRTA"
     gene            complement(10780..11517)
                     /gene="cysT"
                     /locus_tag="VF752_04590"
     CDS             complement(10780..11517)
                     /gene="cysT"
                     /locus_tag="VF752_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX8854858.1"
                     /translation="MWLSVIVLLPLAAVVAKSTEGGLDGFWQAVSSREAVAAIKFTLI
                     ASIVVVAVNAVMGTVIAWVLVRDEFPGKRFVSALIDLPFALPTIVAGLTLLALWGPRS
                     PVGVNLAFSKAAVILALLFVTLPFVVRAVQPVLIELDREMEEAAASLGASPLVIFRRI
                     VLPNLRPAIVAGCSLGFARALGEFGAVVLISGNIPFKTEVASVFVFSQIGNDNVTGAA
                     AVSVVLLLLSLITLGLVARLGRVRHEG"
     gene            complement(11597..12622)
                     /locus_tag="VF752_04595"
     CDS             complement(11597..12622)
                     /locus_tag="VF752_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8854859.1"
                     /translation="MSQFKRPRSFALAALAVMCIALVAAGCGGSSSSSSGGGSGKLSL
                     VAYSTPQEAYAKIIPDFQKTSPGKGVKFTQSYGASGDQSRAVAAGLPADVVALSLEPD
                     VTKLVDGGLVSPDWNKDKWHGFVTDSVVALAVRKGNPKHIETWADLVKPGVQVVTPNP
                     FSSGGAKWNIMAAYGSQIEQGKTPAQAQTFLQAVLKNTVVQDKSARDALQTFTSGKGD
                     VLITYENEAITAQQKDQQLDYVIPDQTILIQNPVAVVKNGNTAKAQAFIDFLHTSTAQ
                     KVFAEKGYRSTDPALVDKKTYPTPAGLFKIDKFGGWSAVNDKFFDPQNGIVTKIEQGL
                     GVSTAKG"
     gene            complement(12793..14217)
                     /locus_tag="VF752_04600"
     CDS             complement(12793..14217)
                     /locus_tag="VF752_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854860.1"
                     /translation="MRPRVVLGAIFAIAAMFAAAAPSEAAPFHSDNVQLLGKLPEAVG
                     AISARFQGQHMYVTTTNGLNVYDISQPESPKLVGALPLPHFENEDVDLGGDTLIISND
                     PSEGVGVLYVIDISDPTNPTIRSVMPNGLIGGLNTVFGLGDSYTGHIANCLQGCDWLW
                     LTGTSQGVAVVDLRDKDNPKFVKAFETPAPKQRGTEVNPGDGGFTHDVFVDRAGIAWI
                     TGQDGTFGYDAADPVNPKLVYRSDENVFNSGQDGPSGDGSNPIDFLHHNSIRTGLFAD
                     RKTKPQRSSTRGGLGDVVAVTEEDYTRPTCQGQGSLQTWQITSDRNSDGTRKLRMLDM
                     WTTELNELANQTGRSPATGLCSAHWFDEDRGLLAQGWYDQGVRFTDISNPRDIRQVGY
                     WVTTGTFWAAYFAPTDPDREVVYGLDLTGGVDVLHIDRGGDGDPSGSPAVKAPVLDRW
                     FTSGPVAGTASSKWGFVCRVPSLQ"
     gene            14349..14942
                     /locus_tag="VF752_04605"
     CDS             14349..14942
                     /locus_tag="VF752_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEX8854861.1"
                     /translation="MTDRKPGRWPSPAILISLLAIVLALTGTATAAGVIKIKKPSQLA
                     TGVVQSRAIADKGVGTKELLPSALGALDGKPGARGPDGARGPAGPVGEKGIAGDGAST
                     LWAVVEPNGGAVRKTSHVEDTFNLTTPSNKYEINFDRNVSLCEYGVTVDGSTSPFPGR
                     ATVNRALLANDKVIVFTYNAAGSPARLGFNVVVDCPT"
     gene            14944..15525
                     /locus_tag="VF752_04610"
     CDS             14944..15525
                     /locus_tag="VF752_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854862.1"
                     /translation="MRKRPSASFVISLIALVLAATGTATAAGVIKIKKPSQLGKNVVT
                     SKALADGKAVGIRDLTRSARQQLKGKDGARGAAGEPGGIGPQGDRGPKGDSPPALWGV
                     VSSTGALTQGVHAISASKTATGAYVVTFDQNVAGCAYLASIGPRDGSIAEPAQGEITV
                     NAGSAATDVVVHTYQFAPPGSAEDHPFHLGVYC"
     gene            15622..16191
                     /locus_tag="VF752_04615"
     CDS             15622..16191
                     /locus_tag="VF752_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854863.1"
                     /translation="MKRLRLRRPSPGLVVAVIALVCATAGGATAATLITGKQIKNSSI
                     TGVDVKNKSLTKADFKGSVTGPPGARGPAGPQGPPGSTAFGSLHYVTSGTVANPNGTQ
                     SFAEAVCNSDERVVGGGVLTSGAFVADQRVNSSFPSDGTGNALGAFGTAGWAAFVDNH
                     GTDTQNFEVWAICAKATAVTKAAGKASKR"
     gene            16333..>17674
                     /locus_tag="VF752_04620"
     CDS             16333..>17674
                     /locus_tag="VF752_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX8854864.1"
                     /translation="MSVLAGLVTLSLLPSAAHAAGPNLHAPGQQVERIGAVASGGVRF
                     QRYQQEIDGVPVIGSQATVTDAPGSRGDLLIDRTRSRLPSDRPSAAIAGSRAIELARD
                     ALGVRSLRGPEAARLALLPESGGVRLVWRVTIPSRDPLASFEALIDARSGELVRSRNL
                     LQHFTGSANVFDPNPVVTQGSTSGLSDSNDADSVALTNLRQTKTLERLDASTCLSGQW
                     VQALRGPGDTPVCRASRDWTDVTRHDPQFEALMAYFHLDREQSYIQLLGFTNVMNRQI
                     VVHTDATTSDNSFFDALARDITTGTGGVDDAEDAEVIEHEYGHAIQDDQVPGFGFPDE
                     DSGAIGEGFGDYISSAMSATFTPNPTFDPCFAEWDTLGSGDTAPVPCLRRVDGTQTVS
                     QGHAACGTEVHCMGEVWSGALWAIRNAIGGATADRLVIQSHFSLTQDPTFNDASR"
BASE COUNT         2987 a         6212 c         5906 g         2569 t
ORIGIN      
        1 ggcggccaag atcggctgcc cggtcatcgg gatcaacgac tccggcggcg cccgcatcca
       61 ggagggcgtc gtctcgctcg gcgcctatgg cgacgtcttc gtgcgcaacg tcaagtgctc
      121 gggcgtcatc ccgcagatct cgctgatcat gggaccgtgc gcgggcggcg ccgtctactc
      181 cccagcgatg accgacttca tcttcatggt gaaggagacc tcgcacatgt tcatcacggg
      241 ccccgacgtg atcaagacag tcacgggcga ggagccgacg ttcgaggagc tcggcggcgc
      301 gatgacgcac aacacgaagt cgggcgtcgc gcacttcgcc gctgacgacg aggagcagtg
      361 cctcgaggac gtgcgctacc tcatgtcgtt tttgccgcag aacaacctcg agacggcgcc
      421 gcgcgtcgac tcgggcgacg accccgagcg gatggacctc gagctcgaca cgatcgtccc
      481 ggacaacccg aacaagccgt acgacatgcg cgatgtgatc caccgtgttg tcgacaacgg
      541 cgagttcttc gaggtgcacg agcactacgc gcgcaacatc gtggtcggcc tgtcgcggct
      601 gaacggctat cccgtgggca tcgtcggcaa ccagcccgcc cagctcgcgg gtgtgctcga
      661 catcgacgcg tccgtgaagg gcgcgcgctt cgtgcgcttc tgcgacgcct tcaacatccc
      721 gctgatcacg ttcacggacg tgccgggctt cctcccgggc acgacccagg agtggggcgg
      781 catcatccgg cacggcgcga agctgctcta cgccttcacc gaggcgaccg tgccgaagat
      841 caccgtggtc acgcgcaagg cgtacggcgg cgcgtacgac gtcatgaact cgaagcacat
      901 gctcgccgac ttcaacttcg cctggccgac cgccgaggtg gcggtgatgg gcccggaggg
      961 cgcggtcaac atcatcttcc ggcgcgacat ctcgagctca cccacgccgg acgagcgccg
     1021 ccagaagctc atctccgact acaaggcgcg cttcgccaac ccctactcgg cggccgagcg
     1081 cggctacatc gacgacgtgc tgatcccgca cgagacgcgc ccgcgcctgt gccaggcgct
     1141 cgacatgctg cagacgaagc gcgtcgagcg gccgaagcgc aagcacggga acatcccgct
     1201 ctaaccgtcg ggaggcgccc cttcgagccg gcgcgcgcgc ggcgcggcgc ggttacgatc
     1261 cccggtccac gattggcagg gggtgcctcg aagtggtccg aagctgggga attcggagga
     1321 tgcgccgttg cgtcgcggcg ctgttcggtg ttgcgattgg gctggcgttg ctgccggccg
     1381 cgaccagtgc ggcccctccc ggcatgcgcg acgtgctcgt cgtcggcaac aactgggaag
     1441 gcacggcgga cgtggtcgac gcgcgcacgt tcgaacggct gacgcggctc aacatcatcc
     1501 ccgacaaggc cgaacggatg gccgagatcc agaccgaccc ggcggcgctc ggcttcttca
     1561 ccgggatcaa cctgcttatc ggcgagggcc acgaccagta cgtcgacgac gccttcacct
     1621 cgcacgacgg gcgctacctc tacgtctcac gaccgagctt caaggacgtc gtcgcgttcg
     1681 acctctccac gcgccaaatc gtgtggcgct tccaggtcga gggctaccgc gcagatcaca
     1741 tggcgatctc gcccgacggc gcgcgcctgc tcgtatccgc ctcgaccgcg aacaaggtgc
     1801 acgagatcga cacggcgaca ggcgaggaga tcgggcagtt cccctccggc gactcgccac
     1861 acgagaacaa cttctcgcgc gacgggcagc tgatctacca cgcgagcatc ggccgcgttt
     1921 acacgcccct cgacgatccc gccttcgacg cgacgaaggg cgaacgctac ttccaggtgg
     1981 tcgacgcgaa cacgctccag atcctcaagc gcgtggacat gggcaagaag ctcgccgagg
     2041 ccggctaccc gaacatgagc tccgcggtgc ggccaatggc gctggcgccg gacgagaaac
     2101 gcgtgtacct gcagatctcg ttcctgcacg ggtttgtgga gtacgacctc gagcacgaca
     2161 aggtgctgcg ggtcgcgaat cttccggtca gcgaggaggc gcagaaactc cgccgcgacg
     2221 agtacgtgct cgactccgcc caccacgggc tcgcgatgaa ccctgagggg acgaagctgt
     2281 gcgtggcggg cacgatgtcc gactacgcgg caatcgtctc gcgccggaca ttcgcgtacc
     2341 agatcgccgc gcacggcgag aagccctact gggccacgaa caacggcgac ggccgctact
     2401 gcttcgtctc gttcagcggc gacgaccgcg tgtcggtcat ctcctacgcg aagcagaagg
     2461 aggtcgcgag catcccggtc ggcgaccacc cgcagcggat gcggatgggc gtcatgcgcg
     2521 aggagtacct gcagccgccc gcttagagcc ggaccgtctg agcacgggcg gcgccctggc
     2581 gatgcgcgtg catcaccgcc tgacagatgg cgaggtcctg gatcgcgagt cccgtcgagt
     2641 cgaacagcgt catctcctcg gccgactcgc gcccgggcgc gcggccggtg agcacgtcgc
     2701 cgagctcggt cacgcgctcg cggtcgacca cgccggccgc caccgcgccc gtgagctcgc
     2761 cgccgtgcga ggcctgcgcc cactcgtcgc agaacagcct gcagcggaca acggcctcga
     2821 cctccgcctc ggccttgccc ggcccgtcgg ccccgagcat gttgaggtga actcccgggc
     2881 gtagctctgg cgcgtgcacc accggctcgt gccccggcgt cacgcaggtc accacgtcct
     2941 gctcgagcgc ctcctcgcgg ctgcccgcct cccagccgag ctcgccggcg agcgcgcccg
     3001 ccgcgtcggg atccgggtcg aagcagaccc cggggccgta ctccgccgcc gcgaggcagc
     3061 gcgccgccca cgcgccgtac acgccgcagc cgatgatccc gacgttccgt gcgtcttcgc
     3121 gcgcaagctc ctgagccgcg atcgccgcca cggcgccggt acgaagcgcc gtcacggcgc
     3181 gcgcatccac cagcccgagc ggctcgccgt catcggagct cgacacgcac accacgccca
     3241 tcaccaccgg cacgccggag gccgggttgc ccggaaacga ggtgacccac ttcaggatcg
     3301 cgaggccgcc gcccttcgcg ggcatcgccc ggaagtcgcc gttaggcacg tcgaggtaga
     3361 gcttcggcgg catcgcccac tcgccgctcg cgaactgcac aaagccgttg cggaccgcgt
     3421 cgatcgcctc cgcgggagac accgcggcga ggaccgagtc gtaatcgaag acagggatct
     3481 ccggcatggg ctggcacggt accctccccg tccgtgagca ggaacggcaa gcacgctccg
     3541 gcgcgcccgc gcatcgagct gtcctacggc agcgcctcac cagaggaggc ggcggcgatc
     3601 acggccgcga tcgagcagtt cctgcgggac accgcaccgg cacccgctac gcacagccgc
     3661 ccgatcgacc gctggcagcg cgccgcgctg ttcgagggcg ccggcttggc gggtgacgct
     3721 ccttcaccct ggggcgacga ccagccctgg ggccacgcta ccgtccctcc ccatggccac
     3781 tgacgagcag cagacaccca cgccggcgac ggaattgcct ccggacgagg tcgatacgac
     3841 cagcgtccag atcgtcgacg tacgcacgga cgaggagcgc gcggcggccc acattccggg
     3901 cgacgtctac atcccgctcg agcgcctgca ggaagacgcc ggcactctcg accgcgagcg
     3961 cacggtcgtc ttctactgcc gtggcggtga ccgctcgcgc gcggccgccg aggccttccg
     4021 cgcctccggc tgggacgcat acagcgtcgc cgggggcctc gtggcgtggg ccgaacgtgg
     4081 cctcccggtg gagccggaag gcgcgcaggt ggttcagcgc agccagctgc cgccgtatta
     4141 ggcgcgcgct gcaaggctaa tttccgaaaa ctcgctcggg ttttgttata ttcgcccgac
     4201 gcgctttgac ccgcgccgag ctacgtcccc gcgcaggaga acgacgacgc atggaaccga
     4261 ccacggtcaa ggccccaggc aggaagggca cgctcgagaa tcctgaggtc tacgagaacg
     4321 tgcctgggca cgtgatcccg atcctcgagc gcgagttcga cgacttcgac aacgaggccg
     4381 agaaattcct cgccggcgac acgcccgagg accagttcat cggcttccgc ctcaagcagg
     4441 gcgtctacgg ccagcgccaa gccgacgtgc agatggtccg ggtgaagctg ccgatgggcg
     4501 gcatcacgcc cgagcagatg gagaccttcg cggacgtgat cgaggagtac gcgccgctcc
     4561 gcaagggcca catcaccacc cggcagaaca tccagatcca ccacgtgccg ctcccggacg
     4621 ccgccaagct gatccgcagg atctcggacg cgggcttgtc ctcgcgagag ggctgcggca
     4681 acacagtccg caatgtgacc ggcgacccat gggcgggcgt gagcgagggc gagctattcg
     4741 acatcacgcc gtatgccggc gcctacgtcc gctacttcgt gcgccatccc acgacgcagc
     4801 tgatgccgcg caaggtgaag acggcgttta cggcgaccga cgaggaccgc gcgatcaccg
     4861 gtatccacga catcgccttc atccctcgca tccgcgacgg agtgagaggc gtcgaggtcc
     4921 gcgtcggcgg cggcacatcg atcatgccgc gcatcgctcc cacgctctac gagttcgtcg
     4981 agctcgacaa cggcgactac ctgaaggtcg ccgaggcggt gttccgcatc ttcgaccgcc
     5041 aggagtggct acgtgtgaac cgcgcccgcg cacgcatcaa ggtgctgatc gacaagattg
     5101 gcatcgacgc cttccgcgag caggtcgagg aggagcttca gggcgactgg ctggccgagc
     5161 gcaacttcga ccccacgccg ctgctcttga tccacgacga ggaggcgaac gcaccggccc
     5221 gggggctcgc ctacgcgcag cccaacggcg acctgtcgga gttcgagcgc ttcagcgcct
     5281 cgaacgtgca ggcgcagcgc caggaagggt tctcgtccgt ccaggtgcgc gtgccgcgcg
     5341 gcgacctctc gcccgagcag ctccgcgggc tcgcgcagat catgcgcgac tactccggcg
     5401 gctacgcccg caccacggtg caccagaact tcctgctgcg ctgggtgcgc gacgagtcgg
     5461 tctacgaggt ctgggacgcg ctcggcaagc tgggcctgaa cgccgccggc gcagacgaga
     5521 tcgtcgacgt ggtcagctgc ccgggcacgg acagctgcaa gctcgggatc acgagctcga
     5581 tggggctcaa tcgggccgtc caggagcgcg tggcatcgat gcagatcgat gacccgctca
     5641 cgaagcgcat ccacatcaag atgagcggct gcccgaacgg ctgcagccag caccacatcg
     5701 cgaacatcgg cttctacggc gcctcgatca aggtcggcga caagacgatg cccgcgtacg
     5761 tcgcgcacat cggcggtcag tacgagggcg gcgaggtcgt ctacgggaac cgcctcaagg
     5821 tgcgcctccc ggccaagcgc gtgccggacg cggtcgagcg gtggctgcgc tggtacgagg
     5881 aagaccgcaa cgacggtgag gagttcaacg cgttcgccga gcgcgtcggc accggcgagt
     5941 tcgaggcccg cgtcaaggac ctggcgatgc ccgtcgagtt caacctcgag aacctggggc
     6001 acttcatcga ctggaaccgc cgcgacccgt acaaggtcga gcgcggcgaa ggcgagtgcg
     6061 cggtctgaca ccgtggcgat agccgcgaca ttcgacgtcg agcacctcga cgcggaagac
     6121 gtcctcgccc acgtcgtcga gcgcttccac cctcgcctct ccgtggcctg ctccttccag
     6181 aaggaggcct cggtgatcat ggacatgctc ctgaagatcg agccggaggc gcgcttcttc
     6241 acgctcgaca ccggcgtgct cttccccgag acctacgcca cctggcgaaa ggccgaagag
     6301 cgctacggga tcaaggtcga cgtgtatcag ggcatgagcc tcgcgcgcca ggccgacctc
     6361 cacggggacg agctctgggc gcgcgacccc gacgcctgct gcggcatccg taaggtcgcg
     6421 ccgctcgacg aggcgctggc ggacgtggac tgctggatct cgggccttcg ccgcgaccag
     6481 agccccacgc gcgcgaacac acccaagctg cactgggacg agaagcacgg cctccacaag
     6541 gcgaatcccc tggccgactg gtcggagaag gacgtctgga cctacatcgc cgagcacgac
     6601 gtcccctaca acccgctgca cgaccagggc tacccgtcga tcggctgcac gtactgcacg
     6661 ctcgccggcg acggtcgcga cggtcgctgg gccggcacgg gcaagaccga gtgcggactg
     6721 cacggctgaa gacaagaaag gacccatgag cgacggctac accctctggt tcaccggcct
     6781 gtcaggggca ggcaagtcca cgatcgcggg catcgtcgag agcgagctgc gccttcgcgg
     6841 gcagaaggtc gaggtgctcg acggcgacgt cgtgcgcacc aacctctcga agggcctcgg
     6901 cttctccaag gaggaccgcg acacgaacat ccgccggatc gccttcgtag cggatctgct
     6961 ctcccgcaac gacgtggcag cgatcaccgc ggcgatctcg ccctaccgcg agacgcgcga
     7021 cgaggcgcgc gcgctgcacg gtgagcgctt catcgaggtc tatgtgaagg catccgtaga
     7081 cgagtgcgcg cggcgcgacg tgaagggcct ctacgagaag gccttcgccg gcgagatcaa
     7141 ggagttcacc ggcgtctccg acccgtacga ggagccgctc gccgccgaga tcgtggtcga
     7201 caccgaggcc gagactccgg aggagtcggc agccaaggtg ctcgcctaca tcgacgcgcg
     7261 agaccgctcg aacggcaacg gccacgtggc gcggaagcag gaggagcccg catggcaagc
     7321 atcgaactga acgcgcgcca gaccgcggac ttcgagctgc tcgcaaacgg cggcttcgcg
     7381 ccgctgacgg gcttccaggg ctccgccgac tggcagtccg tgatcgagcg catgcgcctg
     7441 gccgacggcc agccgtggcc gataccggtc acgctggcga gcgagtcggg cgaagtcggc
     7501 gagacgctcg agctgatcgg ccacgagggc acccgcctcg gcacgatcac gatcgaggag
     7561 gtctacgacc gcgacctcca gcgcgaggcc gagctcgtct acaagacgac cgaggacgct
     7621 cacccgggcg ttgccgcgct ctaccgcgag ccggcacgca cgatcgccgg gccgatctcg
     7681 gctgagcagc tgccgtcgca catcgatgcg gtgcagccgt acctgctgac gcccgctgag
     7741 gcgaaggcgg cgttcgcgga gcgcggctgg aagacggtgg tcgcgttcca gacgcgcaac
     7801 ccgatccacc gcgcgcacga gtacatcacc aagggcgcgc tcgagctggt cgacggcctc
     7861 ttcatccacc cgatcgtggg cgagacgaag tcggacgaca ttcccgccga cacgcgcatg
     7921 cgctgctacg aggtgctgat cgagaactac tacccgcgcg atcgggcggt gctcggcgtg
     7981 aaccccgccg cgatgcgcta cgcagggccg cgcgaggcga tcttccacgc gctcgtgcgc
     8041 cgcaactacg gctgcaccca cttcatcgtg ggccgcgacc acgcgggcgt cggcagctac
     8101 tacggaacct acgacgcgca ggagatcttc gacgagttcg agcccggcga gctgggcatc
     8161 gagccgatga agttcgagca cgcgttcttc tgcaaggtca ccaagaccat ggcgacggcg
     8221 aagacctcgc cctcgactcc ggagcagcgg gtgcacctgt cgggcaccgc cgtgcgcgag
     8281 ctgctcggcc gcggcgagcg gccgcccgag gagttctcgc ggccggaggt ggccgacgtg
     8341 ctgatcgagg cgtacgcagc cgaaggcgag cgggccgcgg cgtagccatt tcgtttctac
     8401 cgtggtcgat tttgccgcgt agagcggcac gatcgaccac ggtacgaaga agcgccccaa
     8461 acgggcccgc ctcccttatt gctgctgtaa gcgcggagag cccaggaagg cgctacgaaa
     8521 tcgacgtaat acacagccgc cgatcccccc gcggcgtatg caccaccacg gtctcaccgg
     8581 cccgatggcc ggccaacgcc cgcccgaccg gtgaatcgaa cgacagcgtc ccgtcgccag
     8641 gcctcgcctg ccccgccgag gtcaaccgaa accgctgact agcgccgctc gtctcgtcga
     8701 cgaactccac agtcgcaccg aggccggcca cgcccgcggc cgccgccgcg ccctcctcca
     8761 cgacggaggc gttctgcagc cggtcgcgca ggcgcaggat gcgcgtctcc aggtgagcct
     8821 gctcctcctt tgccgcgtgg tactcggcgt tctccttcag gtcgccccac tcgcgcgcgg
     8881 tcttgatccg ctcggcgatc tggcggcgcc cttcgccctc gaggcggtcg atctccccgc
     8941 ggaggtcgtc gagctcggcc tgcgtcatca gcttctcagt cattccctgc cctaggtttc
     9001 gagtccgttg aggcaggcag catacgctgc gcctgctcag gccagcgagg cgtagtccgg
     9061 ctcgcgagcc ctcacgtaga gcgtctcgcc cggagaaggg tcgagctcgg cggcctgcgc
     9121 gcgcgtgatc tgcgcgttca gctggccgtt gccgggctgc gacagctcca cccgcacctc
     9181 gaagccgagc gcgacaacgc gctccacgac cgcctccgac gcaccaggct ccggctcgcg
     9241 cgagagctcg aggtcgtgcg gacgcacggc cgagccgtcg agcttcgtca ccgggccgac
     9301 gaaccccatt acgaagtcgg acgcggggcg gtcgtacacc tccgcggggc tgccggtctg
     9361 ctcgatgcgc ccgtcctgca tcacggcgat ctgctcggcg acctccatgg cctcctgctg
     9421 gtcgtgcgtg acgaacaccg tggtcacgtg gatctcgtcg tgcaggcgcc gcagccattc
     9481 gcgcagctcc gagcgcacgc gcgcgtcgag cgcgccgaag ggctcgtcga gcagcagcac
     9541 gtcgggctgc acggccagcg ctcgcgcaag cgccatccgc tgccgctggc cacccgagag
     9601 ctgcgccgga tagcggtcgg cccagccgcc caagtgcacc agcgcgagca gttcgcgcac
     9661 gcgcgcctcg atcgcgttct tcggcagctt gcggatcttc agcccgaacg ccacgttgtc
     9721 gcgcacggtc atgtgcttga aggccgcgta gtgctggaac acgaacccca cgccgcgccg
     9781 ctgagccggt gcgtcggtca cgtcctcgcc gccgatccgc acgctgccct cgtccggcag
     9841 ctcgagcccc gcgatcaccc tcaggagcgt ggacttgccg ctgccgctcg gccccaggag
     9901 cgccgtgagc gatccgctcg gcacgttgag ggacacgtcc gtgagcgccg cgaagtcgcc
     9961 gaagctcttg gagatcccga aaacctcgat gcccatcagg cggtcctcct tctcctctgg
    10021 agcaaagtca tgacggcgac cgtcgacagc gcgatcgcgg cgagcacgac cgacgcggcg
    10081 taggcgccgg ccaggtcgaa gcgctcgaag tggtcctgca cgaagagcgt gagcgtctcg
    10141 gtctgcccgg caagcctgcc cgacacgacg ctcacggccc caaactcgcc gacagcacgc
    10201 gccgtggtga gaacgacgcc gtagcccacg ccccacctga tcgcgggcag cgtgatgcgg
    10261 ctgaacgtct gccacggcga ggcgccgagc gtgcgcgccg cctcctcctg ctcggtgccg
    10321 atctcgcgca gtacgggcgc gacctcgcgc accacgaacg gcagcgagac gaagatcgtc
    10381 gcgaggatca tgccgggggt cgagaagatc actcggatcc cgttttggct gagccagtcg
    10441 ccgagccacc ccgtgcgccc atagacgagg atgagggcga gcccgaccac cactggcgaa
    10501 acggcgaacg gcacgtcgat cagcgcctca acgaccgcct tgccgcggaa gcgcttgcgc
    10561 acgatgagca gcgccgcggc gatcccgaag atggtgttga gcggcaccgc gaccacgacc
    10621 atcagcagcg tgagctggag cgcgtgcagc gcgtcgggcg tggtgaccgc gtcccaggcc
    10681 gcggcgaagc cgtgctcgaa ggcgcggtag aagaccatgc cgaccggcac cacgagcagg
    10741 acgaagaggt agccgagggc gagcgagcgg aggccgaatc tagccctcat gccgcaccct
    10801 cccgagccga gcgaccagcc ccagcgtgat cagcgagagg agcagcagga cgactgatac
    10861 ggcggccgcg ccggtcacgt tgtcgttgcc gatctgcgag aagacgaaca ccgacgcgac
    10921 ctcggtcttg aacgggatgt tcccgctgat cagcaccacg gctccgaact cgccgagggc
    10981 ccgcgcgaag ccgagcgagc agccggcgac gatggcgggg cgcaggttgg gcaggacgat
    11041 gcggcggaag atcacgagcg gcgaggcgcc gagcgacgcg gccgcttcct ccatctcgcg
    11101 gtcgagctcg atgagcacgg gctgcacagc gcggaccacg aacggcagag tcacgaacag
    11161 cagcgcgagg atcaccgcgg ccttgctgaa cgcgaggttc acgccgaccg gcgagcgcgg
    11221 cccccagagg gcgagcagcg tgaggccggc cacgatcgtg ggcagggcga acggcaggtc
    11281 gatcagcgcg ctgacgaagc gcttgccggg gaactcgtcg cgcacgagca cccacgcgat
    11341 cacggtgccc atcacggcgt tgaccgcgac gaccacgatc gacgcgatca gcgtgaactt
    11401 gatcgccgcg acggcctcgc ggctgctgac cgcctgccag aagccgtcga gaccgccttc
    11461 ggtcgacttc gccaccaccg ctgccagcgg cagcagcacg atcacgctca gccacaccag
    11521 cacgatcccg cccgccagtg gcggacggga tcgcgaacgt gagcgggggc gcggccggac
    11581 ggccgcgggg atggcctcag cccttggcag ttgagacccc cagcccctgc tcgatcttcg
    11641 tgacgatccc attctgggga tcgaagaact tgtcgttgac cgcgctccag ccgccgaact
    11701 tgtcgatctt gaagagcccg gccggagtcg ggtacgtctt cttgtccaca agcgcggggt
    11761 cggtcgagcg gtagcccttc tcggcgaaca ccttctgcgc cgtcgacgtg tgcaggaagt
    11821 cgatgaacgc ctgcgccttc gccgtattcc cgttcttgac gaccgccaca gggttctgga
    11881 tgaggatcgt ctggtcgggg atcacgtagt cgagctgctg gtccttctgc tgcgcggtga
    11941 tcgcctcgtt ctcgtaggtg atcagcacgt cgcccttgcc ggacgtgaac gtctgcaggg
    12001 cgtcgcgggc gctcttgtcc tgaaccaccg tgttcttgag caccgcctgc aggaacgtct
    12061 gggcctgagc gggcgtcttg ccctgctcga tctgcgagcc gtaggcggcc atgatgttcc
    12121 acttcgcacc acccgagctg aaggggttcg gcgtgaccac ctgcacgccg ggcttcacca
    12181 gatcggccca ggtctcgatg tgcttcggat tgcccttgcg cactgcgagc gcgacgaccg
    12241 aatcggtcac gaagccgtgc cacttgtcct tgttccagtc cggcgacacc agcccgccgt
    12301 ccacgagctt cgtcacgtcg ggctcgagcg agagcgcgac gacgtcggcc ggcagcccgg
    12361 ccgcaaccgc gcgggactga tcgcccgagg caccgtatga ctgcgtgaac ttgacgccct
    12421 tgccgggaga ggtcttctgg aagtccggga tgatcttcgc gtacgcctcc tgcggagtgg
    12481 agtacgcgac gagcgacagc ttgccgctgc ccccgccgga cgacgagctg ctcgagccgc
    12541 cgcagccggc cgcgaccagg gcgatgcaca tcaccgcgag tgcggcgagt gcgaatgatc
    12601 ggggtcgttt gaactggctc atcggttgga gtcctccgtc ggggatcccg gcgcccagcc
    12661 gggcgcgccg cttggcgcga aggcggcata ttaaccgaaa gccagtcgag ttttcacctt
    12721 ttttggccga aagggcaaga gaaagccgct ccaggagcgg ccttcccgtc attcctcgct
    12781 gtccggcagg atctactgaa gcgacggcac gcgacagacg aagccccact tcgagctggc
    12841 tgtgccggcc accgggccgc tcgtgaacca gcggtcgagc accggcgcct tcaccgccgg
    12901 cgagccggaa gggtcgccat cgccgccacg gtcgatgtgc agcacgtcga cgccgccggt
    12961 tagatcgagc ccgtagacca cctcgcggtc cggatccgtg ggcgcgaagt acgcggccca
    13021 gaaggtgccg gtcgtgaccc agtagcccac ctggcggatg tcgcgcgggt tcgagatgtc
    13081 cgtgaagcgc acgccctggt cgtaccagcc ctgtgcgagg aggccgcggt cctcgtcgaa
    13141 ccagtgggcc gagcagagcc cggtcgcggg cgagcggccg gtctggttcg cgagctcgtt
    13201 cagctcggtg gtccacatgt cgagcattct gagcttgcgc gtgccgtcgc tgttgcgatc
    13261 ggacgtgatc tgccaggtct gcagcgagcc ctggccctgg caggtcggac gtgtgtagtc
    13321 ctcctccgtc accgccacca cgtcgccgag gccgccgcgc gtgctcgagc gctgcggctt
    13381 cgtcttgcga tccgcgaaca ggccggtgcg gatcgagttg tggtggagga agtcgatcgg
    13441 gttcgagccg tcgccgctcg ggccgtcctg ccccgagttg aagacgttct cgtcgctccg
    13501 gtagacgagc ttcgggttca ccggatcggc ggcgtcgtag ccgaaggtgc cgtcctgtcc
    13561 cgtgatccag gcgatgcccg cgcggtccac gaacacgtcg tgggtgaagc cgccgtcgcc
    13621 cgggttgacc tcggtgccgc gctgcttggg cgccggcgtc tcgaacgcct tcacgaactt
    13681 ggggttgtcc ttgtcgcgca ggtccaccac cgcgacgccc tgcgacgtgc cggtgagcca
    13741 gagccagtcg cagccctgga ggcagttggc gatgtggccg gtgtaggagt cgccgaggcc
    13801 gaacaccgtg ttgagcccgc cgatcaggcc gttcggcatg accgagcgga tcgtcgggtt
    13861 ggtgggatcg gagatgtcga tcacgtacag cacgccgacc ccctcgctcg ggtcgttcga
    13921 gatgatcagc gtgtcgccgc cgaggtccac gtcctcgttc tcgaagtgcg gcagcggtag
    13981 cgccccgacc agcttcggcg actcgggctg cgagatgtcg tagacgttca acccgttcgt
    14041 cgtcgtcacg tacatgtgct ggccttggaa gcgcgcgctg atcgcgccga ccgcctcggg
    14101 cagcttcccg agcagctgga cgttgtcgga atggaacggc gccgcctcac tgggcgctgc
    14161 cgcggcgaac atggcggcga tcgcgaagat cgcgccgagt accacgcgtg gacgcatttt
    14221 gaaatcgact cccctgccga agttgcgccg aggatgatgc ccaaccgcga gctcggatgc
    14281 gccgctcttg cggggcgagg cgactggcaa tagcgtgtgc tcacctcctg gccttcgatt
    14341 ggactcgaat gacggaccgc aaaccgggcc gctggcccag cccggcgatt ctcatatccc
    14401 tgctcgcgat cgtgcttgcc ctcacgggca ccgccacggc cgccggcgtc atcaagatca
    14461 agaagcccag ccagcttgcg acgggcgtgg tgcagagccg cgcgatcgcc gacaagggcg
    14521 tgggcacgaa ggagctgctc ccctcggcgc tcggcgcgct cgacggcaag ccgggcgccc
    14581 gcggccccga cggcgcgcgt ggtccggccg ggccggtcgg agagaagggc atcgccggcg
    14641 acggcgcctc gacgctgtgg gcggtggttg aacctaacgg cggtgcggtc cggaagacct
    14701 cgcacgtgga ggacacgttc aacctgacca ccccgagcaa caagtacgag atcaacttcg
    14761 acaggaacgt ctcgctgtgc gagtacggcg tgactgtcga cgggtcaacg agcccgttcc
    14821 ccggacgggc gaccgtcaac agggcgctcc tcgcgaacga caaggtgatc gtcttcacct
    14881 acaacgccgc cggctcgccg gcacggctcg gcttcaacgt cgtcgtcgac tgccccacgt
    14941 gaaatgagaa agcgcccgag tgcgtccttc gtcatctccc tgatcgcgct ggtgctcgcc
    15001 gcgaccggta ccgcgacggc agccggcgtg atcaagatca agaagccgag tcagctcggc
    15061 aagaacgtgg tcacgagcaa ggcgctcgcc gacggtaagg ccgtcggcat ccgggacctc
    15121 acgcgctcgg cccggcagca gctcaagggc aaggacggcg cgcgcggcgc ggccggggag
    15181 ccgggtggga tcgggccaca gggcgatcgc gggccgaagg gcgactcgcc gccggcgctc
    15241 tggggcgtgg tgtcgtccac cggggcgctc acgcagggcg tgcacgcgat cagcgcatcg
    15301 aagaccgcga ccggcgcgta cgtggtcacg ttcgaccaga acgtcgccgg ctgcgcctac
    15361 ctggcgagta tcggcccgcg cgacggttct atcgcggagc cggcgcaggg agagatcacc
    15421 gtcaacgccg gctctgcggc cacggacgtg gtcgtccaca cctaccagtt cgccccgccc
    15481 ggcagcgccg aggaccaccc gttccacctc ggcgtgtact gctgaacagc tccgccatca
    15541 atgatcacgc ggcctggcgc gtaagggcgc cttgctgcca tccgtacatg acgaaacgtt
    15601 cccgttcgag gaaggactcg catgaaacga ctgagactgc gccggcccag tcccgggctc
    15661 gtagtcgccg tcatcgcgct ggtctgcgcg actgcgggtg gtgccaccgc ggcgacactg
    15721 atcactggca agcagatcaa gaacagctcc atcaccggcg tcgacgtgaa aaacaagtcg
    15781 ctcacgaagg cggacttcaa gggctcggtc actgggccgc ccggagcgcg gggcccagct
    15841 ggaccccagg gcccgccagg cagcactgcc tttggctctc ttcactacgt aacgagcggg
    15901 acagtcgcca accccaacgg gacccagagc ttcgccgagg ccgtgtgtaa ctcggacgag
    15961 cgggttgttg gtggcggcgt tctcacctcc ggcgcatttg tcgcggacca gagagtgaac
    16021 tcgtcgttcc cgagcgacgg caccggaaac gctctcgggg cgttcgggac agctggctgg
    16081 gccgcgtttg tcgacaacca cggcaccgat actcagaact tcgaagtctg ggcaatatgc
    16141 gccaaggcca ccgcggtcac gaaggcggcg ggcaaagcca gcaagcgcta aatacacccg
    16201 acgcgcacgt gtaggagcgg cgccgcgccg agatccggcg ccgctccagg tagtgcgatt
    16261 gcggtgcccg cgaagcgggg ttagggtcgc cctacgtcgc gcctccttcc cccgaagctg
    16321 cttcgcggcc ggttgagcgt cctagccggc ctggtgacgc tgagcctgct cccctccgcg
    16381 gctcacgccg cgggaccgaa cttgcatgcg cccggtcaac aggtcgagcg catcggggca
    16441 gtcgccagcg gcggcgtgag gttccagcgc tatcagcagg agatcgatgg cgtcccggtc
    16501 ataggcagcc aggccacggt cacggacgcg cccggctccc gcggagacct gttgatcgac
    16561 cgcacacgct cgcgccttcc gtccgaccgc ccgtcggcgg cgatcgcagg gagccgcgcc
    16621 atagagctgg cgcgggacgc acttggtgtg cgttcgctgc gcggacccga ggcggcgcgc
    16681 ctggcactgc tccccgagag cggcggggtg cggctggtct ggcgcgtcac gatcccctcg
    16741 cgagacccgc tcgcgagctt cgaggcgctg atcgacgcgc gcagcggcga gctggtgcgc
    16801 tcgcgcaacc ttcttcagca cttcacgggt agcgcgaacg tgttcgaccc caatcccgtc
    16861 gtcacgcagg gttcgaccag cgggctgtca gacagcaacg acgccgacag cgtcgcgctt
    16921 accaatctgc ggcagaccaa gacgctcgag cggctcgacg cgagcacctg cctgagcggt
    16981 caatgggtgc aggcccttcg aggcccgggt gacaccccgg tgtgccgagc gagccgcgac
    17041 tggacggacg taacccgcca cgaccctcag ttcgaggcgc ttatggccta cttccacctc
    17101 gaccgcgaac agagctacat ccagctcctc ggattcacga acgtgatgaa ccgccaaatc
    17161 gtggtccaca ccgacgcgac cacctcggac aactcgttct tcgacgcgct cgcgcgagac
    17221 atcaccaccg gcacgggagg ggtcgacgac gccgaggacg ccgaggtgat cgagcacgaa
    17281 tacgggcacg cgatccagga cgaccaggtg cccggcttcg ggttcccaga cgaagactcc
    17341 ggcgcgatcg gcgaaggctt cggcgactac atctcttcag ccatgtcggc caccttcact
    17401 ccgaacccca ccttcgaccc gtgctttgcg gagtgggaca ccctaggctc gggcgacacc
    17461 gctcccgtcc cctgccttcg ccgggtcgac gggacccaga cggtctctca aggccacgcc
    17521 gcgtgcggca cagaggtgca ctgcatgggc gaggtctggt ccggcgccct ttgggcgatc
    17581 cgaaacgcga tcgggggcgc cactgccgac cggctcgtga tccagtctca cttcagcctg
    17641 actcaggatc caacgttcaa cgatgcgtcg aggg
//
LOCUS       DASVXS010000078        17982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1001050, whole genome shotgun sequence.
ACCESSION   DASVXS010000078 DASVXS010000000
VERSION     DASVXS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17982
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001050"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2236)
                     /locus_tag="VF752_04625"
     CDS             complement(<1..2236)
                     /locus_tag="VF752_04625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX8854865.1"
                     /translation="MPDLLDGLNESQLRAVTHPGGPLAVIAGAGTGKTRVITTRFAWL
                     VEQGAAPDSVLALTFSAPAAAEMRERLEVLIEQPWEELWVSTFHSFCARLLQEEALEA
                     GVNPFFAPVTPADRLSLLLDRIDDLTLRRHEIRGNPVPLLASFIARIDRLKDEMVTAA
                     DYAAYARRLEQRARDGGDAERTHAARELEFAQVYGDHDRLLAESGSLDFGDLILCAFS
                     MLHEKPHVRARVAGRFRHVLVDEYQDTNFAQGVLLRALVQEHGNVTVVGDDDQSIYRF
                     RGASQKNLVDFQREFPEADVVRLERNYRSGSKILRAATAVVVPDPERISKTLRGGRGG
                     DVRFWRARSDRAQAQAAAAHAEKLIASGVPPQEIAVLVRSVRSEGQVVGAALEERAVP
                     FRLTGAAAYFQRTEVRDLLAWLRLLAAPGDSGAVVRALSRPPIELRSVDIARLTQFAR
                     RRKLDMVAGVRAALDTPTLSPEGRDRALAFLRLYRAASTAFEEMRPDAFVHRLIERIG
                     LRRQQVYAAQADTVERLVNIAKLSELAGAYVRRQPDATPRDFVRYISAVAEAGLREEE
                     ATPTGLPPAVQVMTMHSAKGLEFDHVLVLGLSAARMPGVRRAGPADQIPDELLKERLP
                     GDSRAMHELEMRRLLHVAMTRARRSLVLAWPEGGERSRPSPFYEEARELLGADEEVFE
                     EELFGPAEGLHSTFRMLRDELLDSVPRTGARLGEMRLDTYVDVAQSVVRYLELLKVAA
                     LIERA"
     gene            2298..3371
                     /locus_tag="VF752_04630"
     CDS             2298..3371
                     /locus_tag="VF752_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854866.1"
                     /translation="MARLRRSDCSGPGITRRRRGRGFAYYDEDGEKVDEPEVLERINE
                     LRIPPAWKGVWICPYPMGHLQATGLDARGRKQYLYHSKWRERRDQQKFDDMIAFAHAL
                     PKMRERMDADLARTGMDRERVLAAAVRLLERGFFRVGGEDYAVENETYGLATMKKSHV
                     TLDRDGSIRFDYPSKSGQRQVRSIVAPEVFDIVKQLKQRRGGSDELLAYRDGRTWVDV
                     RSSDINAYLKEATGGDFSAKDFRTWNATVLAAVALAVSPSASASKTSRKRAIARAVKE
                     VALYLGNTPAVCRASYIDPRVFDRFQAGYTISGVIAELGEQNDLERSALHGNVEEAVL
                     DLLAEDVDAEGVESVDEVMEVVA"
     gene            3371..4024
                     /locus_tag="VF752_04635"
     CDS             3371..4024
                     /locus_tag="VF752_04635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8854867.1"
                     /translation="MPTTAILDVDGTLVDTNYQHAIAWFRAFRQHGFVLPVWRIHRHI
                     GMGGDKLVAALTSEAVDEAHGDDIRTAEGALYFSLIAEVEPMRGARELLEDLRRGGHP
                     VVLASSAKAAEVEHYLDLLEARELADGWTTSSDVQATKPDPDLVQAALDKVGAKEAVM
                     VGDTPWDVKAAKSAGIETIGVLTGGFSRAELDEAGAACVFDSIEELRLKLDETPLAS"
     gene            complement(4021..4626)
                     /locus_tag="VF752_04640"
     CDS             complement(4021..4626)
                     /locus_tag="VF752_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX8854868.1"
                     /translation="MSALSESQTVGLRVLVADEDHEALDRLASMLDELGHEVTARAVS
                     VRELSREINETHPDVAIVKLHGDEDHALDLIDELVEEAECAVLALMEVEKPEFVATAA
                     EKGLFGFVAPATPEAIQSEIEVTVRRHGELRRLSDEVEQLETALARRAIIERAKGILM
                     ERHGVDEKEAFQMLRDHARSSNLKLIDVARSVATGHKLLSR"
     gene            4810..5601
                     /locus_tag="VF752_04645"
     CDS             4810..5601
                     /locus_tag="VF752_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX8854869.1"
                     /translation="MTATDPSPAKPLPRNGGRIEQDRRLIERYQQSGDQAARDELVRR
                     FIPLVRQLARRYQRGNEPLEDLVQVASIGLVKALDRFDGSRGTAFSSYAVPTILGELK
                     RYFRDSGWAVHVPRGMQERVLEVNRAVTRLSRELGHSPSAGEVAEATGLETEQVLEAM
                     EAAVAYETVSLDAPRPADEEESEGYERRLGAEDENFELVEYGATLAPALKQLDVRDRL
                     VLHLRFKEDMTQSEIAERIGVSQMHVSRLIRRALTRLRESTAEAE"
     gene            5632..6684
                     /locus_tag="VF752_04650"
     CDS             5632..6684
                     /locus_tag="VF752_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8854870.1"
                     /translation="MRVVVVGATGNVGTSVLRALSAEPQVDSIVGVARRVSDWHLEKV
                     EWQAADIRSSNLVALFEGADVVVHLAWLIQPSRDHAELRSVNVEGSARLFDAVAEAQV
                     PALVYASSVGAYSPGPKDRAVDESWPTDGIQSSFYARHKAEVERLLDRFEQDHPEVRS
                     VRLRPALIFKREAGSEIRRLFAGPFLPSRLVRKEAIPVVPDIPRLRFQAVHSYDVGEA
                     YRLVALGDAAGAFNIAADPVLDPPELARLLDARTVPVPAGLVRTVADLSWRLRLQPTP
                     PGWVDMALAVPIMDPSRARKELGWEPRRSSGEAVLDVLAGIRESPGEETPPLAADAGG
                     PARAREIATGVGRENP"
     gene            complement(6960..8222)
                     /locus_tag="VF752_04655"
     CDS             complement(6960..8222)
                     /locus_tag="VF752_04655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8854871.1"
                     /translation="MALVDARRRARAAYTTRFDRGAGPSGGARLREANQTSARSGGRV
                     AALDGLRGVAALSVLVYHVWLYTTDDSGHPSTWHDHLLRQGYLGLVLFFVLSGYLLYS
                     PWLRAALERRGAPALCRYVRSRLARIAPGYYVALAGASVLMLTANDAPGTKLIDFSQL
                     PLFLVFAQNWSPDTLLRLDPPMWTLAIEASFYVALPLIGLAAIRFARSRRRQLLLLLA
                     LVAAGVLTRALTADYAASEPLLWSLPAYIDYFACGMLAAVAVHDRTIGRTAGCALLAA
                     GAALVAGNAVVHATLRDTAALQAVQDLPAALGLAVLIAVAAASRGRRRLLASRPLENL
                     GRVSYGLYLWHLPVIVWMRGHGLFPNDLVLALAAVLPPALVAATASWHLVERPALGRL
                     RGRKPEPRRLPGPRQPANERRRPLAQAA"
     gene            8437..8901
                     /locus_tag="VF752_04660"
     CDS             8437..8901
                     /locus_tag="VF752_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854872.1"
                     /translation="MALTRQQMDDQVNQHFMYEATDDIDGVVGSFTENEEIRHEAVPS
                     PVGPLTSLAQVRSYYEMLFPSVRGESVTPIMRLYGDDFLVDETLWTGEIIDGHWCLSD
                     GMSGRASARLLHVFEFAADGKIKSERVWIDLAAIQQQLGAAEQPSDIAAAHR"
     gene            complement(9027..9974)
                     /locus_tag="VF752_04665"
     CDS             complement(9027..9974)
                     /locus_tag="VF752_04665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEX8854873.1"
                     /translation="MTSNTPPALDQASFTASVERHRRELHVHCYRMLGSFEESEDLVQ
                     ETFLRAWRRRETLQSRETMRAWLYKIATNACLDALDKRPRTPSANSEVLWLQPYPDEL
                     LDEAEDPHAAVVAKETIELAYLVAIQRLVPLQRAVLILRDVLGCSAKETADVLDTSVP
                     AVNSALQRAHAAMKDHLPAQRSEWPADVDATAAERELLKRYVAYSEHADPHELKRLLS
                     EDVRFSMPPQPGLWEGRDTVVDAWVEGGFGTEAFGSMRCLVTRANRQPAVACYVRGPA
                     DEHHTPLAIDVLTIAGGEVAEIVTFDGSLFGWFGLPESL"
     gene            complement(10768..10841)
                     /locus_tag="VF752_04670"
     tRNA            complement(10768..10841)
                     /locus_tag="VF752_04670"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10805..10807),aa:Ile,seq:gat)
     gene            complement(10982..11836)
                     /locus_tag="VF752_04675"
     CDS             complement(10982..11836)
                     /locus_tag="VF752_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8854874.1"
                     /translation="MGRGLAAILPRSPRDSEGLRQLLVDLVEANPSQPRRDFDPEALL
                     ALSESIKTRGVLQPIVVRPLHGGGFELIAGERRLRAAKMAGLESIPAIVRVAEEGERL
                     ELALIENMAREDLNPVEEARACATLVDDLGVTKEELGRRVGRSRVAISNLIRLLDLPD
                     EALELLEAGELSEGHGRAILMCKDQTERRRLARAARAKGWSVRETEKRAKAAEEGQSA
                     NPRRSQVVIHPDLGDLVAAAEDALTAALAREVKVRIVNGRCRVEFDLDDPREAVELAA
                     RLLHQPAA"
     gene            complement(11886..12644)
                     /locus_tag="VF752_04680"
     CDS             complement(11886..12644)
                     /locus_tag="VF752_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8854875.1"
                     /translation="MGTVYAIANQKGGVGKTTTAVNVAACVAEAGYPTLLVDLDPQAN
                     ATVGVGLPKDAEPSVYDCLCGDATVAEIARPTAIENLHVVPSTPDLAGASVELPQLPG
                     SESRLREVLGGVRDRYLFAILDCPPSLGPLTVNALVAADRVIVPVQTEYFALEGLADL
                     LDTLSLIQRELNPRLTVAGMILTMHDARTRLGQDVEREVRQHFPDLVFDTVIPRNVRV
                     GEAPSFGVPVTRHDPHCTGSLAYLQLAKEVAARG"
     gene            complement(12703..13326)
                     /gene="rsmG"
                     /locus_tag="VF752_04685"
     CDS             complement(12703..13326)
                     /gene="rsmG"
                     /locus_tag="VF752_04685"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX8854876.1"
                     /translation="MRALAAEPDPPTTVRNPDEAVRQHVADSLSGLELEEVRSAKAIV
                     DIGAGAGFPGLVLAAALPRARVDLVEATRRKVEVIERLARAAALANARAIATRAEEWG
                     AAAGHESYDLVTARALAPLGVLVEYAAPLLVEGGTLVAWKGARDERDERGGAAAAQQL
                     GFEPAGVVRVEPFEGARNRHLHAFRKISPTPPGFPRRPGMASKRPLA"
     gene            complement(13417..14106)
                     /locus_tag="VF752_04690"
     CDS             complement(13417..14106)
                     /locus_tag="VF752_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX8854877.1"
                     /translation="MTGEQVDRDSTTAEAEGSTLGEARWNAVKELEPRYPGLTADCVS
                     FETLDEGDADADVPARVRADVDVAAWERIATEEPDEPAERVRALVGRIVLALGLRATV
                     DISEEDDEIRATVNGDDLGLLIGKHGSTIDAVQHLAVRIAFRGDAERKRIVVDAAGYR
                     ARRQAALQRSADRAVDDALSFKRPVELEPMSAADRKIVHDYLAGRGDVTTHSEGDEPD
                     RRLVVSPVRPD"
     gene            complement(14113..15006)
                     /locus_tag="VF752_04695"
     CDS             complement(14113..15006)
                     /locus_tag="VF752_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX8854878.1"
                     /translation="MFLANVLQPLIDFNDAILKFWHDQVGLPWGWSIVALTFCIRLLI
                     LPLTFRQVRSMQALQRLQPELKKMQERYKDDKQRLNEEMMKFYREHKVNPLGSCLPLV
                     LQFPFFISLFYLLRGDKFKADIAGEAGFGFIPNLGEPLTGHTGPLIFIIVLYIATQLG
                     STLVSAQTMTDKNQRRIFMALPFLFVPFVINFPAGLLVYWISTNIWTIGQQLFIRRFL
                     PPPEPLPAGAAAVTSSTPAKAAKAGGGNASKAAEPARPKRRERPQRAKAEASGDGANG
                     TTRKPPPPSPRKKKKRTGRRR"
     gene            complement(15014..15277)
                     /gene="yidD"
                     /locus_tag="VF752_04700"
     CDS             complement(15014..15277)
                     /gene="yidD"
                     /locus_tag="VF752_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX8854879.1"
                     /translation="MSALRVAATGPIRLYQRLISPAIPRRCKYHPTCSEYAVQAVRSY
                     GILRGLVLAAWRLLRCNPWSHGGVDHVEHQTIFRRRDPAPRNS"
     gene            complement(15274..15675)
                     /gene="rnpA"
                     /locus_tag="VF752_04705"
     CDS             complement(15274..15675)
                     /gene="rnpA"
                     /locus_tag="VF752_04705"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8854880.1"
                     /translation="MAPAGEAGRRGRRRRLSRSAEFDRVYREGRSHANRYLVVHSFPR
                     PASEDEEPRLGVSVGRKVGGAVERNRVKRLLREAFWSAGANLPAGHDFVVVARPDAGE
                     LARTRGEEGIEEALCEVLRSAGLSDGGEAAA"
     gene            complement(15678..15812)
                     /gene="rpmH"
                     /locus_tag="VF752_04710"
     CDS             complement(15678..15812)
                     /gene="rpmH"
                     /locus_tag="VF752_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8854881.1"
                     /translation="MKRTYQPKKRKRARTHGFRARMRTRAGRTLLKRRRAKGRKRLTP
                     "
     gene            16350..17693
                     /gene="dnaA"
                     /locus_tag="VF752_04715"
     CDS             16350..17693
                     /gene="dnaA"
                     /locus_tag="VF752_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX8854882.1"
                     /translation="MPEELETTWNLVRRELRQDAPDFTFHIWLEPLELAGRRGDTLYV
                     CAPDHIRTWVRERHQELVRRAVKRALGPAAEVEVVAEDWSPEQLPTEGEPERRGASIP
                     EALNPKYTFEQFVIGDGNRLAHAAALAVAEQPGQAYNPFFIYGEPGLGKTHLLHAIGN
                     YVQAYGNGLRVRYSTIEDFTAEFVRATRGRDTTAFRERFRDPDVLLIDDVQFLADKMR
                     TKEEFFHTFNALYESGRQLVLTSDRPPADHDEFESRLRERFASGLVADVQPPELDVRL
                     AILRKRARLDALNSVPEETLFEIARSVTASVRALEGALIRVIAYASLRGESATPDLAS
                     KVLATLYPCAASAAPKPTVDAIQAAAAEEFGLDTRALLAHDRRPNIAFARQVAMYLAR
                     ELTDETLPAIGRRFGRNHATVLHAHRKIAEELQSDDKARSAVDNLRRRLRGEASL"
BASE COUNT         2845 a         6306 c         6208 g         2623 t
ORIGIN      
        1 tcgcccgctc gatcagcgcg gccaccttca gcagctcgag gtagcgaacg accgactgtg
       61 ccacgtccac gtacgtgtcg agccgcatct ctccgagccg cgcgccggtg cgcgggacgc
      121 tgtcgagaag ctcgtcgcgc agcatccgga acgtggagtg gagtccctcg gccgggccga
      181 agagctcctc ctcgaagacc tcctcgtcgg caccgagcaa ctcgcgcgcc tcctcgtaga
      241 agggcgatgg ccgcgagcgc tcgccgccct ccggccaggc gagcacgagc gacctgcgcg
      301 ctcgtgtcat cgccacgtga agcagccggc gcatctcgag ctcgtgcatg gcgcgactgt
      361 cgccgggcag acgctccttc agcagctcgt ccgggatctg gtcggccggc ccagcacggc
      421 gcacgcccgg catccgcgct gccgacaagc ccagcacgag cacgtggtcg aactcgagcc
      481 ccttcgccga gtgcatcgtc atcacctgca ccgcgggcgg gaggccggtc ggcgtcgcct
      541 cctcctcgcg caggcccgcc tcagcgacgg ccgagatgta gcgcacgaag tcgcgcggcg
      601 tggcatccgg ctggcggcgc acgtacgcgc cggcgagttc ggacagcttc gcgatgttca
      661 cgaggcgctc gacggtgtcc gcctgcgccg cgtacacctg ctgacggcgc aggccgatcc
      721 gctcgatcag gcggtgcacg aacgcatccg ggcgcatctc ctcgaacgcg gtcgacgccg
      781 cccggtagag ccgcaggaag gccagcgcgc ggtcgcgtcc ttcgggcgag agcgtcggcg
      841 tgtcgagggc agcgcgcacg ccggccacca tgtcgagctt gcgccggcgc gcgaactgcg
      901 tgagccgcgc tatgtcgacg gagcgcaact cgatcggcgg gcgcgacagg gcgcgcacca
      961 ctgcccccga gtctccgggc gcggccagca gccgcagcca ggcgagaagg tcgcgcacct
     1021 cggtgcgctg gaaatacgcc gcggcgccag tcagcctgaa aggaacggct cgctcctcga
     1081 gcgccgcgcc gacgacctgt ccctcgcttc gcaccgagcg gacgagcacc gcgatctcct
     1141 gcggcggaac gcccgaggcg atcagcttct cggcgtgcgc cgccgcggcc tgcgcctgcg
     1201 cacgatcgga ccgcgcgcgc cagaagcgca cgtcgccgcc gcggccgcca cggagggtct
     1261 tggagatccg ttcgggatcg ggcacgacca ccgcggtcgc cgcccgcagg atcttcgatc
     1321 ccgagcggta gttgcgctcg agccgcacga cgtccgcctc ggggaactcc cgctggaagt
     1381 cgaccaggtt cttctgcgac gcgccgcgga agcggtagat cgactggtcg tcgtccccga
     1441 cgaccgtcac attcccgtgc tcctgcacca gcgcgcgtag cagcacgccc tgcgcgaagt
     1501 tcgtgtcctg gtactcgtcg accagcacgt ggcggaagcg gccggcgacc cgcgccctca
     1561 cgtgcggctt ctcgtggagc atgctgaacg cgcacaggat gaggtcgccg aaatcgagtg
     1621 agccgctctc ggccagcagg cggtcgtggt cgccgtacac ctgcgcgaac tcgagctcgc
     1681 gcgccgcgtg cgtgcgctcg gcatcgccgc cgtcgcgcgc ccgctgctcg agccgccgcg
     1741 cgtacgccgc gtagtccgcc gccgtgacca tctcgtcctt cagccgatcg attcgcgcga
     1801 tgaagctcgc gagcagcgga accggattgc cgcggatctc gtggcggcgc agcgtgaggt
     1861 catcgatccg gtcgagcagg agcgacagcc ggtcggcggg cgtgaccggc gcgaagaacg
     1921 ggttgacgcc tgcctcgagc gcctcctcct ggagcaggcg cgcgcagaag gagtggaagg
     1981 tggagaccca aagctcctcc cacggctgct cgataagcac ctcgagccgc tcgcgcatct
     2041 cggcggcggc gggtgcggag aatgtcagcg cgagcaccga gtcgggcgca gctccctgct
     2101 ccaccagcca cgcgaagcga gtcgtgatca cgcgcgtctt gcccgtgccg gcacccgcga
     2161 tcaccgcaag cgggccgccc gggtgggtga cggccctgag ctgcgattcg ttcaggccgt
     2221 cgagcagatc tggcacggct caaggatacg tccgcgcgaa gggttgcccg gcgccattcg
     2281 agggaaccat cggcgccatg gccagactgc gccgctccga ctgctcgggc cccggaatca
     2341 cccgccggcg gcgtggccgc ggcttcgcgt actacgacga ggacggcgag aaggtcgacg
     2401 aacccgaggt gctcgagcgg atcaacgagc tgaggatccc gccagcgtgg aagggcgtat
     2461 ggatctgccc gtacccgatg gggcacttgc aggcgacggg cctcgacgcg cgcgggcgca
     2521 agcagtacct gtaccactcg aagtggcgcg agcggcgcga ccagcagaag ttcgacgaca
     2581 tgatcgcgtt cgcgcatgcg ctgcccaaga tgcgtgagcg tatggacgcg gacctcgccc
     2641 gcacaggcat ggaccgcgag cgcgtgctcg ccgcggccgt ccggctgctc gagcgcggct
     2701 tcttccgcgt cggcggcgag gactacgccg tggagaacga gacctacggt ctcgccacga
     2761 tgaagaagag ccacgtgacg ctcgaccgcg acggctcgat caggttcgac tacccgtcga
     2821 agagcggtca gcggcaggtg cgctcgatcg tcgcgccgga ggtcttcgac atcgtgaagc
     2881 agctgaagca gcgccgcggc ggcagcgatg agctgctcgc ctaccgcgac ggtcgcacgt
     2941 gggtggatgt gaggtcctcg gacatcaacg cgtacctgaa agaggcgacc ggcggcgact
     3001 tctcggcgaa ggatttccgc acctggaacg cgaccgtgct tgccgccgtc gcgctcgccg
     3061 tgtcgccaag cgccagcgcg tcgaagacct cgcgcaagcg cgcgatcgcg cgcgccgtga
     3121 aagaggtggc gctctatctc ggcaacacgc cggcggtctg tcgcgcctcg tacatcgatc
     3181 cacgtgtctt cgatcgcttc caggcggggt acacgatcag cggagtgatc gcggagctcg
     3241 gcgagcagaa cgacttggag cgctctgcgc tccacggcaa cgtggaggaa gcggtgctcg
     3301 acctgctcgc cgaagacgtg gacgccgagg gtgtcgagtc ggtcgacgag gtcatggaag
     3361 tcgtcgcctg atgccgacca cggcgatctt ggacgtcgat ggcacgctcg tcgacacgaa
     3421 ctaccagcac gccatcgcgt ggttccgcgc gttccgccag cacggcttcg tactgcccgt
     3481 gtggcgcatc caccggcaca tcgggatggg tggcgacaag ctcgtcgccg cgctcacgag
     3541 cgaggcggtc gacgaggcgc acggcgacga catccgcacc gccgaaggtg cgctctactt
     3601 ctcgttgatc gcggaggtcg agccgatgcg gggggcgcgc gagctgctcg aagatctgcg
     3661 ccggggcggt catcccgtcg tgctggccag ctcggccaag gccgcggagg tggagcacta
     3721 cctggacctg ctcgaggcac gcgagctcgc cgacggctgg accacgtcat ccgacgtgca
     3781 ggcgacgaag ccggaccccg acctcgtgca ggcagcgctc gacaaggttg gcgcgaagga
     3841 ggcggtgatg gtcggcgaca cgccgtggga tgtgaaggcc gcgaagagcg ccgggatcga
     3901 gacgatcggg gtgctcaccg gcggcttctc gcgcgctgag ctcgacgagg caggcgcggc
     3961 ttgcgtgttc gactcgatcg aggagcttcg cctgaagctc gacgagaccc cgctcgcgag
     4021 ctagcgcgac agcagcttgt ggcccgtcgc caccgagcgc gcgacgtcga tgagcttgag
     4081 gttgctgctc cgcgcgtggt cgcgcagcat ctgaaacgcc tccttctcgt cgacgccgtg
     4141 gcgctccatg agaatcccct tcgcgcgctc gatgatggcg cggcgggcga gcgcggtctc
     4201 gagctgctcg acctcgtccg acagccggcg cagttcgccg tggcggcgga ccgtgacctc
     4261 gatctcgctc tggatggcct cgggcgtagc cggagcgacg aagccgaaga ggcccttctc
     4321 cgcggccgtg gccacgaact cgggcttctc cacctccatc agtgcgagca cggcgcactc
     4381 cgcctcctcc acgagctcat cgatcaagtc gagcgcgtga tcctcgtcgc cgtgcagttt
     4441 cacgatcgcg acgtcggggt gcgtctcgtt gatctcgcgc gacagctccc gcaccgacac
     4501 ggcgcgcgcg gtcacctcgt gaccaagctc gtccagcatc gacgcgagcc gatcgagcgc
     4561 ctcatggtcc tcgtcggcca cgaggacgcg aaggccgacc gtttgcgact cactgagggc
     4621 gctcacatcc ccttcttgcg gtagttctcg ctgctatcgt tgtgggccag ttccggccca
     4681 gcaccccgcc ggccagcttc gcgcccgaga ccgccggcgc ctgttctttc gctgggtaac
     4741 cggattgggg cgcacacatt ggtcgacatc cccgcagcgc tgtcgtggct ttcagggcca
     4801 ggctttgcca tgacggccac agatcctagc cccgcaaagc cgctgccccg caacgggggg
     4861 cggatcgagc aggaccgcag gctgatcgag cgctaccagc agagcggcga ccaggccgcc
     4921 cgtgacgagc tggtgcgccg cttcatcccg ctggtgcgtc agctcgcgcg tcgctaccag
     4981 cgcggcaacg aacctctcga ggacctcgtg caggtcgcga gcatcggcct cgtgaaggcg
     5041 ctcgaccgct tcgacggcag ccgcggcacg gccttctcga gctacgcggt accgacgatc
     5101 ctcggcgagc tgaagcgcta cttccgggac tccggctggg cggtgcacgt gccgcgcggg
     5161 atgcaggagc gcgtgctcga ggtgaaccgc gccgtgaccc ggctctcgcg tgagctcggc
     5221 cattcgccca gcgcgggcga agtggcggag gcgaccgggc tcgagaccga gcaggtgctc
     5281 gaggcgatgg aggccgcagt ggcgtacgag accgtgtcgc tcgacgcgcc gcgcccggcc
     5341 gacgaagagg agagcgaggg ctacgagcgc cgcctcggcg ccgaagacga gaacttcgag
     5401 ctggtcgagt acggggccac gctcgcgccg gcgctgaagc agctcgacgt gcgtgaccgc
     5461 ctcgtgctcc acctccgctt caaggaggac atgacgcagt cggagatcgc cgagcgcatc
     5521 ggcgtctctc agatgcatgt gtcccgcctg atccgacggg cgctcacccg cctgcgcgag
     5581 agcacagccg aagcagagtg agctatcgct cgcccgggta gctccgcgcc gatgcgtgtc
     5641 gtcgtggtgg gagcaaccgg caatgtaggg accagcgtgc tgcgcgcgct cagtgccgaa
     5701 ccgcaggtgg actcgatagt gggtgtcgcg cggcgggtgt ctgattggca cctcgagaag
     5761 gtggagtggc aggcggccga catccgcagc tcgaacctcg tcgcgctgtt cgagggcgcc
     5821 gacgtggtgg tccatctcgc ctggctgatc cagccgtcgc gtgaccacgc cgagctgcgc
     5881 tccgtgaacg tcgagggaag cgcgcgcctc ttcgacgctg tggccgaggc gcaggtgccg
     5941 gcgctcgtgt acgcgtcgtc cgtgggcgct tactcgccgg gcccgaagga ccgcgcggtg
     6001 gacgagagct ggcctacgga cgggatccag tcatcgttct acgcgcgcca caaggccgag
     6061 gtcgagcgcc tgctcgaccg cttcgagcag gaccaccccg aggtgcggtc ggtgcgccta
     6121 cggccggcgc tgatcttcaa gcgggaggcg ggctccgaga tacggcgcct gttcgccggc
     6181 cctttcctcc ccagccggct ggtgcgcaag gaggcgattc cggtggtgcc ggacatcccg
     6241 cgcctgcgct ttcaggcggt tcactcgtat gacgtaggcg aggcgtaccg gctcgtcgcg
     6301 ctcggcgatg cggccggcgc gttcaacatc gccgccgacc ctgtgctcga cccgcccgag
     6361 ctcgcccgcc tgctcgacgc acgcacggtg ccggtgcccg cagggctcgt gcgcacagtc
     6421 gccgacctga gctggaggct gcgccttcag ccgacgcccc cgggctgggt ggacatggcg
     6481 ctggcggttc cgatcatgga cccgtcgcgg gcgcgcaagg agctcggctg ggagccccgg
     6541 cggagctcgg gcgaggccgt gctcgacgtg ctggcgggca tccgcgagtc gccaggcgag
     6601 gagacccctc cgctcgcggc ggacgcgggc gggccggcgc gcgcgcgtga gatcgcgacc
     6661 ggtgtcgggc gcgagaaccc gtaatcggac gagccggaaa tcaaacgagc gaggtgctcc
     6721 cgcaacacct cgctcgtaaa acctcccgcg ctgtcgcccg gacccgtccg aatgaagtct
     6781 tccgtgcctt ttgggcagag gcgacatcct cgccgggatg tcgcctcacc cgtgcagctc
     6841 cctcaaccac tcggttctcg ccggcacttc cctgcgccgt gcgcgcttca agtacccctg
     6901 gccacaatgg gcaaaacatc gcccccgggg cgagagtcga ttggcgcctc tgcgctcact
     6961 caggcggcct gggcgagcgg tcggcgccgc tcgttggccg gctgtcgagg ccctgggaga
     7021 cgcctaggct cgggcttgcg cccgcgcaag cgacccagcg ccgggcgctc gaccaggtgc
     7081 cagctcgcgg tagcggcgac cagcgcgggc ggcagcaccg ccgccagtgc cagcaccaga
     7141 tcgttcggga atagaccatg tccccgcatc cacacgatga ccggcagatg ccagaggtag
     7201 aggccgtacg aaacgcgtcc gagattctcg agcggccggc tcgcgagcag ccgtcgcctg
     7261 ccgcgcgacg cggccgccac cgcgatcagc acggcgaggc cgagggcggc cggcaggtcc
     7321 tgcacggctt gaagcgccgc cgtgtccctc agggtcgcgt gcacgacggc gttacctgcg
     7381 acgagcgcag cgcccgccgc aagcagggcg cagccagccg tgcggccgat cgtccggtcg
     7441 tggaccgcga cggcggccag catgccgcac gcgaagtagt cgatgtacgc gggaagcgac
     7501 cagagcagcg gctcgctcgc ggcatagtcg gcagtcagcg cgcgggtcag cacgcctgca
     7561 gccaccagcg cgaggaggag gagcagctgg cgccggcggc tgcgcgcgaa gcggatcgcg
     7621 gcgaggccga tgagcgggag agcgacgtag aagctcgcct cgatcgcgag cgtccacatc
     7681 ggggggtcga ggcgcagcag ggtgtccggc gaccagttct gcgcgaacac gaggaagagc
     7741 ggcagctgcg agaagtcgat cagcttggtg ccgggcgcgt cgttggccgt gagcatgagg
     7801 accgaggcgc cggcgagcgc cacgtagtac ccgggcgcga tgcgcgcgag ccggctccgc
     7861 acgtagcggc agagtgcggg cgcgccgcgc cgctcgagcg ccgcacgcag ccacggcgag
     7921 tagagcaggt agcccgagag cacgaagaac agcacgagtc cgaggtagcc ctgtcgcagc
     7981 agatggtcgt gccacgtgct cgggtggccg gagtcatccg tcgtgtacag ccacacgtgg
     8041 tacacgagca ccgagagcgc ggccacgcca cgcaacccat cgagcgcggc gacgcgtcca
     8101 cccgaacggg cagacgtctg gttcgcctcg cgcagacgcg caccaccgga aggaccagct
     8161 cccctgtcaa accgagtggt gtacgcggcg cgagcccggc gacgggcatc gacaagcgcc
     8221 acgagcccgg tgatacccaa agagccgaag ctctaaataa gcgcggtaag gggtgccgga
     8281 acggggagcg gagccgccga gggcgtcgga caccacacgg gctgcggacg cgacgagtga
     8341 ggcaagtaga cggttgaccc gtctcttcca cggtggttac gcacgaaatc cactgcgagc
     8401 cacgtctgag gactcgtctc agaagggagc ggcgtcatgg ccctgacgcg ccaacagatg
     8461 gatgaccagg tcaaccagca cttcatgtac gaggccaccg atgacatcga cggggtcgtc
     8521 ggcagcttca ccgagaacga ggaaatacgc cacgaggcgg tcccatcgcc ggtcggcccg
     8581 ctcacgagcc tggcgcaggt gaggtcctac tacgagatgc tcttcccatc cgtcaggggc
     8641 gaaagcgtca cgccgatcat gcgcctctac ggggacgatt ttttggtcga cgagacgctc
     8701 tggacgggcg agatcatcga cggccactgg tgcctctccg acggcatgag cggccgcgcg
     8761 agcgcccggc tgctccacgt gttcgagttc gccgccgacg ggaagatcaa gagcgaacgc
     8821 gtgtggatcg acctcgccgc catccagcaa cagctcggcg cagctgagca accaagcgat
     8881 atcgcggccg cgcatcgata agtaaggcct tgcaactcga taaacggggt ggagagccgc
     8941 ggacggcccc agcactacgc ggggcgagaa atccgaaggt ctgcagcgct ggttgctgac
     9001 gagcagcggc cgcgactcgc ggcgccctag agcgactccg gtagcccgaa ccagccgaac
     9061 agcgacccgt cgaaggtgac gatctcggcg acctcgccgc ccgcgatggt cagcacgtcg
     9121 atcgccagcg gcgtgtggtg ctcgtcggcc gggccccgga cgtagcacgc gaccgccggc
     9181 tgccggttcg cgcgcgtgac gaggcagcgc atcgagccga acgcctccgt gccgaacccg
     9241 ccctcgaccc aagcgtcgac aacggtgtca cggccctccc acagaccggg ctgcggcggc
     9301 atcgagaagc gcacgtcctc gctgagcagg cgcttgagct cgtgcgggtc cgcgtgctcg
     9361 ctgtaggcga cgtagcgctt gagcagctcg cgctcggctg cggtggcgtc gacgtcggcc
     9421 ggccactcgc tccgctgcgc gggcaggtgg tccttcatcg ccgcgtgcgc gcgctgcaac
     9481 gcgctgttga ccgcgggaac gctcgtatcg agcacatcgg cggtctcctt cgccgagcag
     9541 ccgagcacgt cgcgcaggat cagcaccgcg cgctgcagcg gcaccaggcg ctggatcgcg
     9601 acgaggtacg cgagctcaat cgtctccttg gcgacgaccg cggcgtgcgg gtcctcggcc
     9661 tcatcgagca gctcgtcggg atacggctgc agccacagga cctcgctgtt cgccgacggc
     9721 gtgcgcgggc gcttgtcgag cgcgtcgagg cacgcgttcg tggcgatttt gtagagccag
     9781 gcgcgcatcg tctcgcgaga ctgcagcgtc tcgcgccggc gccacgcgcg caggaacgtc
     9841 tcctggacga ggtcctcgga ctcctcgaac gacccgagca tccggtagca gtggacgtgc
     9901 agctcgcgac ggtggcgctc gacggaggcg gtgaaggagg cctggtcgag ggcggggggt
     9961 gtgttggagg tcatgtcagg tgtgacggcg ccgcggcgaa aactcatcgc tgccgcgaat
    10021 gatgagcagg ctcacgcacg tcaggcgcag cccgagcgac gaagaactca ccgccatatc
    10081 tgccgatccg cgcgtcgacc ccgacacggc ggtctccgag cggcggtcgc ccgctcggat
    10141 cagcgcaagt tatgcaccaa gtagtgcctc aacgatcgac tcagctgcgt cgaagggcgc
    10201 acgcagtgca cccctcgagg tggtgccgct cggcaagaaa ccccggtgag ttgtaactca
    10261 ctgataccgc tcggttcctg tcctcccaca ccaacacctt gagtgggata tccaacgcag
    10321 caagcggcgc cgcgagcatc accgtcgcgc ccacagtcgg cttcccaaac atgacgagtt
    10381 tcgagtcgcg catctgcaaa tcagctcggt cggctacgcc gctgtgatcg atgacgttga
    10441 accccgtgaa ggcccgatcc gcgatcagac gcctgagtcc gtcgatggtc tgtgggacgg
    10501 acctgctgct gggttcggtg atgaccccgt ccgaagattg cgagctgtct ggtatcaggc
    10561 ttcctttcga agtgaggcac agttgtgccc ggctgcgaga gcaggtcgtt gtctgattct
    10621 cgttcaaaat gcgtggctcg gagaaggtgt cggccgagga agagatccgc cgggcccgcg
    10681 cgaagatccg gcccacgagg cgaggagccc gctgcagcgg aggcccccgg acacgaagaa
    10741 ggccccgctg tgcagggcct tcgcgagtgg gcgatccagg actcgaacct gggacctctt
    10801 ccttatcaga gaagcgctct aaccggctga gctaatcgcc cgcggcccgc gatgatagcg
    10861 gcgcccacgg ggcccgtcag ggcggcgaaa agcgacgagg ccggtgtccg gcgagaccgt
    10921 ggcgtacgga cgtacgcgag ggctcgccgt acgtcgggat ccgacgcttt gcagccgccc
    10981 gtcaggccgc aggctggtgc agtaggcggg cggcgagctc gaccgcctcg cgcgggtcgt
    11041 cgaggtcgaa ctcgacgcgg cagcggccgt tgacgatgcg caccttcacc tcgcgcgcga
    11101 gcgccgcggt gagcgcgtcc tcggccgcag ccacgaggtc gccgaggtcc ggatggatga
    11161 ccacctgcga cctgcgcggg ttcgccgatt gcccttcctc agccgccttc gcgcgcttct
    11221 ccgtctcgcg cactgaccat cccttcgcgc gcgctgccct tgcgagccgg cgccgctcag
    11281 tctggtcctt gcacatgagg atcgcgcggc cgtgtccctc cgacagctcg ccggcttcga
    11341 gcagctcgag cgcctcgtct ggcaggtcga ggaggcggat caggttcgag atcgccacgc
    11401 ggctgcggcc cacccgccgg ccgagctcct ccttcgtcac gcccaggtcg tcgacgagcg
    11461 tcgcgcaggc gcgcgcctcc tcgaccgggt tcagatcctc gcgcgccatg ttctcgatga
    11521 gcgcgagctc gaggcgctcg ccctcttccg cgacgcggac gatcgccggg atcgactcga
    11581 ggccagccat cttcgccgcc cgcaggcggc gttcgccggc gatgagctca aagccgccgc
    11641 cgtgcagcgg gcgcacgaca atcggttgaa gcacgccgcg cgtcttgatg gattccgaca
    11701 gcgccagcag cgcctcgggg tcgaagtcgc gcctcggctg gctcggattg gcttctacga
    11761 ggtctaccag aagctggcgt agaccctcag agtcacgcgg gcttcgcggc aagatggcgg
    11821 cgagtccgcg gcccatgcca cgaggttgcc ctgcattttg cagacgctca ccctgaggtg
    11881 caggctcagc cacgtgccgc cacctccttc gcaagctgca ggtaggcgag cgagcctgtg
    11941 cagtgcgggt cgtgccgggt gaccgggacg ccgaagctcg gcgcctcgcc gacccggacg
    12001 ttgcgtggga tgaccgtgtc gaacacgagg tccgggaagt gctggcgcac ctcgcgctcc
    12061 acgtcctggc cgagccgcgt gcgcgcgtcg tgcatggtca ggatcatccc ggcgaccgtg
    12121 aggcgcgggt tcagctcacg ctggatgagc gacagcgtgt cgagcaggtc ggcaagaccc
    12181 tcgagcgcga agtattcggt ctgcaccggg acgatgacgc ggtcggcggc gacaagggcg
    12241 ttgaccgtga gcggtccgag cgacggcgga cagtcgagga tcgcgaacag gtagcggtcg
    12301 cgcacgccgc cgagcacctc gcggagccgc gactcggagc cgggcagctg cggcaactcg
    12361 acgctcgcgc cggccaggtc gggcgtggag ggcaccacgt gcaggttctc gatcgcggtc
    12421 ggtcgcgcga tctcggcgac cgtcgcatcg ccgcagaggc agtcgtacac cgagggctcg
    12481 gcgtccttcg gcaggcccac ccccaccgtg gcgttggcct gcgggtcgag atcgaccagc
    12541 agtgtcggat agccggcttc cgccacgcag gcagcgacgt tgacggcggt cgtggtcttg
    12601 ccgacgccgc ctttctggtt ggctatcgcg tagacggtgc ccatcgcccc cggaaggtag
    12661 cgaggaccgc ggtcagaact agattcgttc gggtgcgaac ggtcaggcca gtggccgctt
    12721 ggacgccatc ccagggcggc gcgggaagcc cggcggcgtg ggcgagatct tgcgaaatgc
    12781 gtgcagatgg cggttgcgcg cgccctcgaa cggctcaact cgcaccacgc cggccggttc
    12841 gaagcccagc tgctgagccg ccgccgcacc gccgcgctcg tcgcgctcgt cacgggcgcc
    12901 tttccacgcc accagagtgc cgccctccac gagcagcggc gcggcgtatt ccacgagcac
    12961 gccgagcggc gcgagcgccc gcgcggtaac caggtcgtag ctctcgtgac ccgctgctgc
    13021 gccccattcc tccgcgcggg tggcgatcgc gcgcgcgttc gcgagcgcag ccgcccgcgc
    13081 caagcgctcg atcacctcga ccttccgacg cgtcgcctcg acgagatcca cgcgcgcgcg
    13141 aggaagcgcg gcggcaagca cgagccccgg gaagccggcg cccgccccga tatccacgat
    13201 cgcctttgcg gagcgcacct cttcgagctc cagcccgctc aggctgtcgg cgacgtgctg
    13261 ccgaaccgct tcgtccgggt ttctcaccgt cgtaggcggg tcgggctcgg ccgccagcgc
    13321 ccgcaacagc gcctcaaagc gatcggagac cccagcagca agcccgtagc ggccggcgag
    13381 agcgtcgagc gtttcccgtg aaacgctcag gacaggtcag tcgggccgga cgggtgagac
    13441 gaccaagcgc cgatcgggct cgtcgccttc gctgtgcgtg gtcacgtcgc cgcgaccggc
    13501 gaggtagtcg tggacgatct tgcggtcagc ggccgacatc ggctcgagct cgaccgggcg
    13561 cttgaaggag agcgcgtcgt ccacggcccg gtccgcgctc cgctgcagag ccgcctggcg
    13621 gcgcgcgcgg tagcccgccg catcgaccac gatccgcttg cgttccgcgt cgccgcggaa
    13681 ggcgatccgc acggcaagat gctggaccgc gtcgatcgtg ctgccgtgct tgccgatcag
    13741 caggccgaga tcgtcgccgt tcaccgtggc gcggatctcg tcgtcctcct cgctgatgtc
    13801 caccgtggcc cgcaggccga gcgcgagcac gatccgccca accagcgcgc gcacgcgctc
    13861 cgccggctcg tcgggctcct cggtggcgat ccgctcccac gccgccacgt ccacgtccgc
    13921 ccgcacgcgc gctggaacat cagcgtccgc gtcgccctcg tcgagcgtct cgaagctcac
    13981 gcagtcggcg gtgagcccgg ggtagcgcgg ctcgagctcc ttgacggcgt tccagcgcgc
    14041 ctcgccgagc gtcgagccct cggcctcggc cgtcgtgctg tccctgtcca cctgctcccc
    14101 cgtcacctgc ggctaccggc ggcggccggt cctctttttc ttcttgcgcg gggagggagg
    14161 cggcggcttc cgggtcgtgc cattggcacc gtcaccgctg gcctccgcct tcgcgcgctg
    14221 aggccgctca cgccgcttcg gacgagccgg ctcggccgcc ttcgacgcgt tgcctcctcc
    14281 cgccttcgcc gccttggcgg gcgtggacga cgtgacggca gcggcgccgg ccgggagcgg
    14341 ctccggcggc ggcaggaagc gacgaatgaa cagctgctgc ccgatcgtcc agatattggt
    14401 gctgatccag tagacgagca ggcccgccgg gaagttgatc acgaacggga cgaacaggaa
    14461 cggcagcgcc atgaagatcc ggcgctggtt cttgtccgtc atggtctgcg ccgacacgag
    14521 cgttgagccg agctgcgtcg cgatgtagag cacgatgatg aagatgagcg ggccggtgtg
    14581 cccggtcagc ggctcgccca ggttggggat gaagccgaag ccggcttcgc ccgcgatgtc
    14641 cgctttgaac ttgtcgcccc tcagcaggta gaagagcgag atgaagaacg ggaactgaag
    14701 gacgagcggg aggcacgacc cgagcgggtt caccttgtgc tcccggtaga acttcatcat
    14761 ctcctcattg aggcgctgct tgtcgtcctt gtagcgctcc tgcatcttct tgagctccgg
    14821 ctggagccgc tgcagcgcct gcatcgagcg cacctgccgg aaggtgagcg gcaggatcag
    14881 cagccggatg cagaaggtga gcgccacgat cgaccaaccc cacggcagcc ccacctggtc
    14941 gtgccagaac ttcaggatcg cgtcgttgaa atcgatcagc ggctgaagga cgttggcgag
    15001 aaacatgggc tggtcaggag ttgcgtgggg ccgggtcgcg ccggcggaag atcgtctggt
    15061 gctcgacgtg gtcgacgccg ccgtggctcc aaggattgca gcggagcaga cgccaggcgg
    15121 cgagtacgag ccctcgcagt atgccgtagg agcgcaccgc ctgcaccgcg tactcggagc
    15181 acgttggatg gtacttgcag cgcctcggga tcgccggcga tatcaacctc tggtagagcc
    15241 ttatcggccc ggtggcagcc actcgcagcg cgctcatgcc gccgcctcgc cgccatcgga
    15301 caagccggcg gaccgcagga cctcgcacag cgcctcctcg atgccctcct cgccgcgcgt
    15361 ccgcgcgagc tcgcctgcgt cggggcgcgc gacgactacg aagtcgtggc cggcaggcag
    15421 gttcgcgccg gcgctccaga aggcctctcg caatagccgc ttgacacggt tgcgctcgac
    15481 ggcgccgccg accttgcggc ccaccgacac gccgagccgc ggctcctcgt cctcgctcgc
    15541 aggtcgcgga aacgagtgca ccaccaggta gcgatttgcg tgcgagcgac cctcgcgata
    15601 gacgcgatcg aactcggcgc tgcgcgacag ccgccgtcgg cggccacgcc gcccggcttc
    15661 gcccgcaggc gccatcacta cggagtcagc cgcttgcggc ccttggcgcg acgccgcttc
    15721 agcagcgtcc gccccgcgcg tgtgcgcatg cgcgcgcgga acccgtgggt gcgagcgcgc
    15781 ttgcgcttct tcggctgata ggtgcgcttc ataagacgaa agggtccttc ctgcgggacg
    15841 gacccgagag tatagggggg ccgattcagc agtctcccta cggcttcgcg cggaagtccg
    15901 gaacgccgcg gagcgggaga ctgctgcctc ggcctaccgc acggaagacc cgattcagca
    15961 ggctgcgcgg gccgcacaga acgcccgtcc actgccaccc cagcccgccg ggcgaacggt
    16021 cctcaagcgt gcactgcacg gattgggcag ccggtgggcg ggaggtggta atcggagggc
    16081 tgctgagccg cgaagcggcg taccgccgag ggccaccacc cgcccaccgg cgtaccgccg
    16141 acacaacgtg cacagcttgt ggacaagttc gaaatgaggg ccccaacgct tttcccgcga
    16201 tttgcgacct ggcgcctgaa ccggcccgcc agcgcgcttc ggcgccggta tacactcgcc
    16261 gcgctgggcc actgagtccc ttcaccggta ggcccgcacc tctccaatag ccgacccagc
    16321 aaccgcccga ggagggcagt cgacgccccg tgccggaaga gctcgagacg acgtggaacc
    16381 tcgttcgccg cgaactccgg caagacgcgc ccgacttcac cttccacatc tggctcgagc
    16441 ccctcgagct tgccggccgc cgcggcgaca cgctctacgt ttgcgccccc gatcacatcc
    16501 ggacctgggt tcgcgagcgc catcaggagc tcgtgcggcg tgcggtgaag cgggcgcttg
    16561 ggccggctgc cgaggtcgag gtcgtcgccg aggactggtc gcccgagcag cttccgaccg
    16621 agggtgagcc agaacgccgc ggagcgtcga ttcccgaggc actcaacccg aagtacacct
    16681 tcgagcagtt cgtgatcggc gacggcaacc gcctcgccca cgccgccgct ctcgcggtcg
    16741 ccgagcagcc ggggcaggcc tacaacccct tcttcatcta cggcgaaccc ggcctcggca
    16801 agacgcacct gctccacgcc atcggcaact acgtacaggc ctacggcaac ggcctgcgcg
    16861 tccgctactc gacgatcgag gacttcacgg ccgagttcgt ccgcgccacg cgcgggcgcg
    16921 acacgacggc cttccgcgag cgattccgcg accccgacgt gctcctgatc gacgacgttc
    16981 agttcctcgc cgacaagatg cgcacgaagg aagagttctt ccacaccttc aacgcgctct
    17041 acgagtccgg gcggcagctc gtactcacga gcgaccggcc gcccgcggac cacgacgagt
    17101 tcgagtcacg cctgcgcgag cgcttcgcct ccggcctggt cgccgacgtc cagccgcccg
    17161 agctcgacgt gcggctcgcg atcctccgca agcgcgcgcg cctcgacgcc ctcaactcgg
    17221 tgcccgagga gaccctcttc gagatcgcgc gcagcgtcac agcaagcgtg cgcgcgctcg
    17281 agggcgcgct catccgcgtg atcgcctacg cctctcttcg cggcgagtcg gcgacccccg
    17341 acctcgcctc caaggtgctc gcaacgctgt atccgtgcgc ggcgagcgcc gcaccgaagc
    17401 ccaccgtcga cgccatccag gcggccgcgg ccgaggagtt cggcctcgac acgcgcgcgc
    17461 tgctggccca cgaccgccgg ccgaacatcg cctttgcacg ccaggtggcg atgtacctcg
    17521 cgcgcgagct cacggatgag acgctgccag ccatcggtcg gcgcttcggc cgcaaccacg
    17581 ccacggtgct gcacgcccac cgcaagatcg ccgaggagct ccagtccgac gacaaggcgc
    17641 gcagcgctgt cgacaacctg cggcgtcgcc tgcgcgggga agcgtcgcta tgaccgatcg
    17701 cactggcaca atccacagct tctcgtcacg ccccgaaccc gtcatgcggg tgcgcaagcg
    17761 caattcggcg ggggaacgaa cttttcaaca cacctacttc cactggacag aaccatcaga
    17821 tgaagttctc tgtcgatcgt gatcagttcc tgacccagct cggcatcgcc gttcggggcg
    17881 tgtccacgcg cagcgctatc caaaccctgt cgggcgtcat gatccgcgcg gctgagggcg
    17941 gtgtggagct ctcgtccacc gatatggaga tcggtgtgcg cg
//
LOCUS       DASVXS010000079         5854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_515033, whole genome shotgun sequence.
ACCESSION   DASVXS010000079 DASVXS010000000
VERSION     DASVXS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515033"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VF752_04720"
     CDS             complement(<1..1278)
                     /locus_tag="VF752_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX8854883.1"
                     /translation="MEHLSEIVLGVMVAVVALSVLARVAGVPYPIVLVIGGLVISLIP
                     GVPEVHMNPDLVLALFLPPLLYSAAFFANLRDIRADLRSISLAAVGLVIATAVVVAVV
                     AHALIDGMPWAVAFALGAIVAPTDPLAATTIAQRLGAPRRLVTIIEGEGLLNDGTALV
                     IYRVAIAAAIGGGFSAGETVLRLFSSAAGGIAIGLAVAWVIAEVRRRLDDVPVEITIS
                     LATGYAAFLPAEAAGVSGVLAAVTAGIYMGWRSPDISTPSMRMQGSAVWQTLQFLLNA
                     VLFVLIGLQLPVVVENLGGYSTTTIIGYAAAVAGAAVGTRFIWVNTTPYIVRALDRRP
                     QQRARRADWRLRFVIFWAGMRGAVSLAAALAIPLETDAGGAFPDRQLIVFLAFGVILV
                     TLVGQGLTLGPLLGALGLAQGDERRRREAVARAR"
     gene            complement(1325..2296)
                     /locus_tag="VF752_04725"
     CDS             complement(1325..2296)
                     /locus_tag="VF752_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8854884.1"
                     /translation="MPARGGIDLGGTKIQAVVIDASNEVLSEARHDTPTSGGPPDVAE
                     AMANALTEAAGAAGVETDSLIGVGVGSPGAVDPAAGTLSNPPNLPGWEGAYPLASVLS
                     EKLGTPASLGNDVTVATDAEAKLGAGRPYSSLLGVFWGSGVGGGIVLDGKEWNGRGAA
                     GEIGHVVVELGGARCGCGRKGCMEAYAGRTRMEAYARKRVEKGDETQLFEIMEKRGRD
                     RLTSSVWARALDHGDPLAHKIMRRAVEALGAGVASCLNVLDVEAVIIGGGLGVRLGED
                     YVRKIEKAVMPHLFMDSRPPDFLLSELGDLGGAIGASLLVTEPARVR"
     gene            complement(2319..3254)
                     /locus_tag="VF752_04730"
     CDS             complement(2319..3254)
                     /locus_tag="VF752_04730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8854885.1"
                     /translation="MRAIQVTRYGGPEVLEPVELERPSPISTEVLVKVHAAGVNPVDW
                     KTRRGTGVSHWVGPPPFVPGWDVAGVVEATGYGVTRIAPGDHVYGMPWFPRAASAYAE
                     YVTAPSRHFARMPEGIGFEEAGAMPLAALTATQALDSAAVEEGTRVLVLAASGGVGHL
                     TVQLAKLRDAHVTAAGSPRNLDFLRELGADEVVDRTTIPVERFASDIDAVIDLVGGDV
                     TGAALATLREDGVLVAVPDGADDATKLEAERRAVSVIEPLVEPDGRILDEIAAFVAGG
                     RLRVVIDRVFALEEAAAAHEHLETGSVRGKVVLRV"
     gene            complement(3254..4630)
                     /locus_tag="VF752_04735"
     CDS             complement(3254..4630)
                     /locus_tag="VF752_04735"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8854886.1"
                     /translation="MAISAEQVLKVVEPATEQVMAELPEAGAQEADEAIERAKAAFPA
                     WRDISPGERAEILRSIAQKIRDNLEDMARIEARNVGKPIGDARGEVGMVADVFNYYAG
                     APERLLGDTIPVAGGVDVTFREPLGVVGLITPWNFPLPIASWKVAPALAAGNTIVLKP
                     AELTPLSALELERLAVQAGLPEGVLNVVVGPGRVAGERMVEHPDVAKVAFTGSTAVGR
                     RVGELASQSIKRVTLELGGKSANIVFADADLEKAAASAPIAVFGNAGQDCCARSRILV
                     QRSVLDRFMEGLEEAVENMRVGDPLDESTEMGPLISANQRETVASYVDDDAPVAIRGQ
                     APDGPGFWYPPTVLAPMSNDDRAAREEIFGPVACVIPFDDEADAIRIANDTIYGLSGS
                     VWTENSARALRVARALETGTISVNSNTSVRVTTPFGGFKQSGMGRELGPHALDYYTEL
                     KNVFIATS"
     gene            4804..>5854
                     /locus_tag="VF752_04740"
     CDS             4804..>5854
                     /locus_tag="VF752_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8854887.1"
                     /translation="MSEALVDSSSTRVLPPELDRRYPVIVRGEGVWMEDSAGKRYLDA
                     MSGGSMALTLGHGRRDLIETAREQAKRLAYVHNERITNPPQERLAHELTSVAPDGFTR
                     VHFVTGGAEANETAMRMARSYHVERGDTSRWRVISPAQSYHGPTMATLALTGRPGLWG
                     PFGPYLSSHLHIPPATWRFDPTGEQALEALDRALEEAGPETVSTFYCEAITAAALPAY
                     TPPERFWKGLDERRREHGFLVCFDEIVTGVGRTGRWFAASDLPIKPDIIATAKGLGAG
                     YAAIGAAICQEHVYEAVANGSRRLTLGHTWDGAPLPCAVGTQVIEILSREGWVTRVNE
                     RGPKLREQLADALGGV"
BASE COUNT          909 a         2123 c         2029 g          793 t
ORIGIN      
        1 ccgcgcgcgg gcgaccgcct cgcgccgccg ccgctcgtcg ccttgggcga ggccgagcgc
       61 gccgagcaac gggccgagcg tgagcccctg gccgaccagc gtcacgagga tcaccccgaa
      121 cgcgaggaac acgatcagct ggcggtccgg gaacgcgccg ccggcgtccg tctcgagcgg
      181 gatcgcgagc gcggcggcga gcgacaccgc gccgcgcatc cccgcccaga agatcacgaa
      241 gcgaaggcgc cagtcggcgc gccgcgcgcg ctgctggggt cggcggtcga gtgcacggac
      301 gatgtagggc gtggtgttga cccagatgaa tcgcgtgcct acggcagcgc ccgccacggc
      361 cgcggcatat ccgatgatcg tcgtcgtcga gtagccgccg agattctcga caacgacggg
      421 tagctgcagg ccgatcagca cgaacagcac cgcgttcagc aggaactgca gcgtctgcca
      481 gaccgcggag ccctgcatgc gcatgctcgg cgtggagatg tccggtgagc gccagcccat
      541 gtagatgccg gcggtcacgg ccgcgaggac gcctgagacg ccggccgcct cggcgggcag
      601 gaaagcggcg tacccggtcg cgagagagat ggtgatctcc accgggacgt cgtcgagccg
      661 gcgtcgcacc tccgcgatca cccaggccac cgcaagcccg atcgcgatgc caccggcggc
      721 gctggagaag agccgcagca ccgtctcgcc ggccgagaag ccgccgccga tcgcggcggc
      781 gatggccacg cggtagatca cgagagccgt gccgtcgttc agcaggccct caccctcgat
      841 gatcgtcacg agccgtcgtg gcgcgcctag tcgctgagcg atcgtggtag ctgccagcgg
      901 gtcggtgggt gcgacgatcg ccccgagcgc gaacgctacg gcccagggca tgccgtcgat
      961 cagcgcgtgg gccacgaccg caactaccac cgccgtggcg atcaccagcc cgacggccgc
     1021 gagcgagatc gacctcaggt cggctcggat gtctcgcagg ttcgcgaaga acgcggccga
     1081 gtagagcagt ggcggcagga acagcgccag cacgaggtcc gggttcatgt ggacctcggg
     1141 aacgcccggg atgaggctga tcacgagccc gccgatcacg agcacgatcg ggtagggcac
     1201 gccggccacg cgcgccaaca cgctcagcgc gacgaccgcc accatcacgc cgagcacgat
     1261 ctcgctcaga tgctccatca gtccaccccg tacggaggag cccgcgccgc cggccgaggc
     1321 cggcctaccg aacccgagcc ggttcggtca cgagcaagga cgcgccgatg gcgcccccca
     1381 gatcgccgag ttccgagaga agaaagtccg gcgggcgcga gtccatgaag aggtgcggca
     1441 tgacggcctt ctcgatcttg cggacgtagt cctcgccgag gcgcacgccc agcccgcccc
     1501 cgatgatcac ggcctcaacg tcgagcacgt tcaggcacga ggccacgccg gcgccgagag
     1561 cctcgaccgc gcggcgcatg atcttgtgcg cgagcgggtc gccgtggtcg agcgcgcgcg
     1621 cccagacgct cgaggtgagc cggtcgcggc cgcgcttctc catgatctcg aacagctgcg
     1681 tctcgtcgcc cttctccacg cgcttgcgcg cgtaggcctc catgcgagta cggccggcgt
     1741 aggcctccat gcaacccttc cggccgcagc cgcaacgcgc gccgccgagc tcgacgacca
     1801 cgtggccgat ctcgcccgcc gcgccgcgcc cgttccactc cttgccgtcg agcacgatgc
     1861 cgccgcccac gccggatccc cagaacacgc cgagaagcga ggagtacggg cggccggcgc
     1921 cgagcttcgc ctcggcatcg gtggcaaccg tcacgtcgtt gccgaggctc gccggggtgc
     1981 cgagcttctc agacagcacc gaggccagcg gataggcgcc ctcccaaccc ggcaggttcg
     2041 gtgggttcga gagcgtgcca gccgcggggt cgaccgcgcc gggcgagcca acgccgactc
     2101 cgatgagcga gtcggtctcg acgcccgctg cgcccgccgc ctcggtgagg gcgttcgcca
     2161 tcgcctcggc cacgtcgggc ggaccgccac tggtgggcgt gtcgtgacgc gcttcactga
     2221 gcacctcatt cgaggcgtcg atcacgacgg cctggatctt cgttccgcct aggtcgatcc
     2281 cgccgcgggc aggcacgcgg caaacgatac gccagcgctt aaacgcgcag aacgaccttg
     2341 ccgcgcacgg agccggtctc gaggtgctcg tgtgcggccg cggcttcctc gagcgcgaag
     2401 acgcgatcga tcaccacccg aagccggccg ccggccacga acgctgcgat ctcgtcgaga
     2461 atgcggccgt cgggctcgac cagcggctcg atcacgctca cggcgcgccg ctccgcctcg
     2521 agcttggtgg cgtcgtccgc gccgtccggg acggcaacga gcacgccatc ctcacgcagc
     2581 gtggcgagcg ccgcgcccgt gacgtcacca ccgacgaggt cgatcacggc gtcgatgtcg
     2641 ctggcgaagc gctcgacggg gatcgtcgtg cggtccacga cctcgtcggc gcccagctcg
     2701 cgtaggaaat cgaggttgcg cgggctcccc gccgccgtca cgtgcgcgtc gcgcagcttg
     2761 gcaagctgca cggtgagatg gcccacgccg ccgctcgccg cgagcacgag cacgcgcgtc
     2821 ccttcctcaa ccgccgccga atcgagcgcc tgcgttgcag tcagcgcagc gagcggcatg
     2881 gcccccgcct cctcgaaccc gatgccctca ggcatgcggg cgaagtgccg tgacggggcg
     2941 gtcacgtact cggcgtaggc ggacgccgcc cgcggaaacc acggcatgcc gtacacgtgg
     3001 tcgccgggcg cgatccgggt taccccgtaa ccggtcgcct cgaccactcc tgcaacgtcc
     3061 cagccgggca cgaacggcgg cgggccgacc cagtgcgaca cgccggtgcc cctgcgggtc
     3121 ttccagtcca ccgggttcac gccggcggcg tgaaccttca cgagcacctc ggtcgagatc
     3181 ggcgacggcc gctcgagctc gacgggctcg agcacctcag gcccgccgta gcgggtcact
     3241 tgaattgccc tcatcagctg gtggcgatga agacgttctt cagctcggtg tagtagtcga
     3301 gcgcgtgcgg accgagttcg cggcccatgc cggactgctt gaagccgccg aatggcgtgg
     3361 tcactcgcac cgacgtgttc gagttcaccg aaatcgtgcc ggtctcgagt gcccgcgcga
     3421 cgcgcagagc gcgcgccgag ttctcggtcc agaccgatcc cgacagcccg tagatcgtgt
     3481 cgttggcgat ccggatcgcg tcggcctcgt cgtcgaacgg gatcacgcac gccaccgggc
     3541 cgaagatctc ctcgcgcgcg gcgcggtcgt cattcgacat cggggcgagc accgtcggcg
     3601 ggtaccagaa gcccggcccg tcgggcgcct ggccgcggat cgccaccggc gcgtcgtcat
     3661 cgacgtagga ggcgacggtc tcgcgctggt tggcagagat gagcggcccc atctcggtcg
     3721 actcgtcgag cgggtcgccg acccgcatgt tctcgacggc ctcctcgagc ccctccatga
     3781 agcggtcgag cacgctgcgc tgcacgagga tgcgcgagcg cgcgcagcag tcctggccgg
     3841 cgttgccgaa cacggcgatc ggcgcggacg ccgcggcctt ctcgaggtcg gcgtcggcga
     3901 acacgatatt ggccgatttg ccgcccagct cgagcgtgac gcgcttgatc gactgcgagg
     3961 cgagctcgcc cacgcgccgg ccgaccgcgg tcgagccggt gaacgcgacc ttcgccacgt
     4021 cggggtgctc gaccatgcgc tcgccggcga cgcggcccgg cccgaccacc acgttgagca
     4081 cgccctcggg cagccccgcc tggaccgcga gccgctccag ctcgagggcg ctgagcgggg
     4141 tgagctcggc gggcttcagc acgatcgtgt tgcccgcggc gagcgccggc gccaccttcc
     4201 aggacgcgat cggcagcggg aagttccagg gcgtgatcag gccaaccacg ccgagcggct
     4261 cgcggaaggt cacatcgacg ccgcccgcga ccgggatcgt gtcgccgagc aggcgctcgg
     4321 gcgcgcccgc gtagtagttg aagacatcgg cgaccatgcc gacctcgccg cgcgcgtcgc
     4381 cgatcggctt gcccacgttg cgcgcctcga tgcgcgccat gtcctccagg ttgtcgcgga
     4441 tcttctgcgc gatcgagcgc aggatctcgg cgcgttcacc cggcgagatg tcgcgccacg
     4501 cggggaaggc tgccttcgcc cgctcgatcg cctcgtcggc ctcctgggcg cccgcctcgg
     4561 ggagctccgc catcacctgc tcggtcgcgg gctcgacgac cttcaatacc tgctcagcgc
     4621 tgatcgccac gggcacctcc ggggtgggcg gtccaaaatg gaaaggtctg caagcgagtc
     4681 ctttagcagc tgagcctgcc gggcgtcaag gcaggagcga tgtcgacgct ctctgcggcg
     4741 cctgatataa ggaccttgat ctggtcctac tgagaagtcc gaccaaagtg gaggaagttg
     4801 cgcgtgagcg aagcgctcgt tgacagctct tcaacccggg ttctgccgcc cgagctcgac
     4861 cggcgctatc cggtgatcgt gcgcggcgag ggcgtttgga tggaggactc cgccggcaag
     4921 cgctacctcg acgccatgag cggagggtcg atggccctga cgctcggtca cgggcgccgc
     4981 gacctgatcg agacggcgcg cgagcaggcc aagcgcctgg cctacgtgca caacgagcgc
     5041 atcacgaacc cgccccagga gcgtcttgcc cacgagctca cgtcggtcgc cccggacgga
     5101 ttcacgcgcg tccacttcgt gaccggcggc gccgaggcga acgagaccgc catgcgcatg
     5161 gcgcgcagct accacgtgga gcgaggagac acgagccgct ggcgcgtgat ctcgcccgcg
     5221 cagtcgtacc acggtccgac catggccacg ctcgcgctca ccggccggcc cgggctgtgg
     5281 gggccgttcg gtccctacct cagcagccac ctgcacatcc cgccggccac ctggcgcttc
     5341 gacccgacgg gcgagcaggc gctcgaggcg ctcgaccgcg cgctggagga ggccgggccc
     5401 gagaccgtgt cgacgttcta ctgcgaggcg atcaccgctg ccgcactgcc ggcgtacacg
     5461 cctcccgagc gcttctggaa aggcctcgac gagcggcgcc gcgagcacgg cttcctcgtg
     5521 tgcttcgacg agatcgtcac cggcgtcgga cgcaccggca ggtggttcgc cgccagcgat
     5581 ttgccaatca agcccgacat catcgccacg gccaagggac tcggcgcggg ctacgccgca
     5641 atcggcgcgg cgatctgcca ggagcacgtg tacgaggccg tcgcgaacgg gtcacggcga
     5701 ctcacgctcg gccacacgtg ggacggtgcg ccgctgcctt gcgcggtcgg cacgcaagta
     5761 atcgagatcc tctcgcgtga aggctgggtg acgcgcgtga acgagcgcgg gccgaagctg
     5821 cgcgagcagc tcgcggacgc gctgggcggc gtgg
//
LOCUS       DASVXS010000080        13794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1294497, whole genome shotgun sequence.
ACCESSION   DASVXS010000080 DASVXS010000000
VERSION     DASVXS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294497"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..753)
                     /locus_tag="VF752_04745"
     CDS             complement(76..753)
                     /locus_tag="VF752_04745"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX8854888.1"
                     /translation="MDIRFLGHACFELADGDTRILIDPFLTGNPKAAASPDELEPTHI
                     FLTHGHQDHYGDIVEIAKRTGAHCVSVTDIAGDLESQGVEQVTNPNFGGTAPFDGGWV
                     RIVPAFHTSTTPNGTVSPAAGLVINLNGTVVYHLGDTSLFSDLALPGRRDDIAVALIP
                     IGGHYTMDRQDAALAAGLVGANTVIPCHYDTFPLIETDAQAFKDDVEANTSSRVVILQ
                     PGETHAV"
     gene            complement(758..1090)
                     /locus_tag="VF752_04750"
     CDS             complement(758..1090)
                     /locus_tag="VF752_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854889.1"
                     /translation="MRERQFTDAEVDDALEALTDPERFSDAEARVAKIAPQLQRILAS
                     ALHSGGWLEAHESEVAKALQSDDPHERMIAVRTLLAEETRLAMLVGVAVGWELARELE
                     IPKDQRKD"
     gene            1110..2123
                     /gene="selD"
                     /locus_tag="VF752_04755"
     CDS             1110..2123
                     /gene="selD"
                     /locus_tag="VF752_04755"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX8854890.1"
                     /translation="MQELKLTALSHGAGCACKLAPGELHALLEEIPRPADADLLVGSE
                     TADDAAVYRVAPDLAIVTTIDFFTPIVDDPYEFGRIAAANALSDIYAMGGEPMTALNV
                     VAWSLEELGREALSEVLRGGADTAAQAGVAIVGGHSIDDREPKYGLAVTGRVHPDRLY
                     RNSTARAGDDLFLTKPLGGGVASTAAKRGVASPELSRRAAEVMMELNREATLLAREAD
                     VSAVTDVTGFGLLGHLHEMTIASGLAAEVDARAVPAIDGVLELLRSDEPPIAGGTRRN
                     RAWVEPHVSFNSNVPAELAWLVCDAMTSGGLLIAARVGSGAPGTRIGRLVEGAAGSIA
                     VSA"
     gene            complement(2113..2880)
                     /locus_tag="VF752_04760"
     CDS             complement(2113..2880)
                     /locus_tag="VF752_04760"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8854891.1"
                     /translation="MSQPDLFVVCRNCGSEVSPYVTECPYCGQRVRKRAPKIERGRRG
                     EEPQRRRRRPTLPRMRRGEIPGIAIEARPYATALLVVASLVATLVAVSGVISSSTDLS
                     IDDMGGLFIKPGDEWWRVLTTPFLHNNAGYAFIALFATGLFGSSLERRFGWWAPLFVF
                     IGSGAAGAALAVALNDLPALGANGAALGLVTAWLVDDRMALGRGDDRESDLIGAYVFA
                     AVLLLVAVAWPDASIFAGVGGAAAGAVFGLGLAQLKR"
     gene            complement(2937..4058)
                     /locus_tag="VF752_04765"
     CDS             complement(2937..4058)
                     /locus_tag="VF752_04765"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX8854892.1"
                     /translation="MEHRFTGPSYTIGIEEELMILDAETLDLSNSIETMLRAVPQREE
                     QVKPELLESVLEVATQPHKNTRDAGEELRALRREVSRTAAEYGLAIGSSGTHPFAMWE
                     DQRVVARSRYRDLIAALRFVARQELIFGAHVHVAVDDPDKAIHVANGMRVHLPMLLAL
                     SANSPFWRADSTGLLSTRTPIFRAFPRVGIPPTYKDFEDFAKRVQFMVDSKVIEDYTY
                     LWYDVRPHPRFGTVEIRVMDAQTRVEHTLAIAALIQALVRELAEHFDAGKRLSQFPYQ
                     MLDENKWLAARHGLDGELVDLPKTGRVQTRALVRRVLHRMREHAQDLGSADDLACIED
                     LLDRGNGASRQVVVYEANHDLKEVVAEIVEKTAPDSAPV"
     gene            4168..5097
                     /locus_tag="VF752_04770"
     CDS             4168..5097
                     /locus_tag="VF752_04770"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX8854893.1"
                     /translation="MTPGYDGKLFILAFDHRGSFQKKWFGIEGEPSEEDVNRIADAKH
                     LVFEGLLAALEQGAEASVTGALVDEQFGGPRNIPQEAKQRGLKVAMPVEKSGQNEFDF
                     EYGDQFGEHIEKFDPDFSKVLVRYNPDDDPDMNRQQLERLKKLADWLHERDRLFLFEL
                     LVPATDQQLESAGGDSERYDAELRPELMRRAIEEIQDFGIEVDIWKIEGVDERSDAEM
                     LAQQTRAGGRDNVVCVLLGRGANDDKVDHWLRQAAPVEGFVGFAIGRSIWGDPLKAFI
                     DGSLEREAAAKQIADNYLRFVRVYEEAEAATPA"
     gene            5139..5387
                     /locus_tag="VF752_04775"
     CDS             5139..5387
                     /locus_tag="VF752_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854894.1"
                     /translation="MATRMRQSLAQFEAAFREEAAEERLRRERLRREAAERSRARRHQ
                     KVQKSGTMRFGVLVFVILATTVIVTIAMFQTLSLLIGG"
     gene            complement(5425..5670)
                     /gene="csrA"
                     /locus_tag="VF752_04780"
     CDS             complement(5425..5670)
                     /gene="csrA"
                     /locus_tag="VF752_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX8854895.1"
                     /translation="MLVLTRKANQSIMIGDDIEVSVLAIMGEKVRIGIEAPRSVPVFR
                     KEVYLEIQQEKAAGAEHADRAEVNEALERLKSSQSGK"
     gene            5940..6512
                     /locus_tag="VF752_04785"
     CDS             5940..6512
                     /locus_tag="VF752_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854896.1"
                     /translation="MARRSLKSQLNQIRSWVREGRTDAWIAHQLEVSVKDIDAFKREH
                     GLAPAGEDGNGDGPRYDDEVDLRAEDDALVAAELEAEPDEDELEEDEEELEEELEEGE
                     RPTRRRRGRRGGRGRKRRAGPRELEGTFDHGDEGYGLWLDPEVQDDAIYAEHWAGHRP
                     VTVTIEPNRIVIRRAGEDVGDNDEDENDED"
     gene            complement(6548..6919)
                     /locus_tag="VF752_04790"
     CDS             complement(6548..6919)
                     /locus_tag="VF752_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854897.1"
                     /translation="MPYPVENALFQWREGERRVRDTDEPARFQLERAADAVLEELRRR
                     LGSSFSVEELADFYAGGTDWADEIAQRWASGTDTTWVVDSAFHRYAREATNYAGGRAR
                     EEIKRPDSGPATAGDRPARRS"
     gene            complement(6965..7333)
                     /locus_tag="VF752_04795"
     CDS             complement(6965..7333)
                     /locus_tag="VF752_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX8854898.1"
                     /translation="MAQATVARSRAAAARRRRPAPPGSRRTGGSGIRWDRVGRMSLLA
                     VLGVILLLYISPLHRYFSQSQTAGRQREELRSLEQENARLKQGIADLRSPQSLERRAR
                     ELGMVKQGERAYVIENIPGR"
     gene            complement(7780..9054)
                     /gene="eno"
                     /locus_tag="VF752_04800"
     CDS             complement(7780..9054)
                     /gene="eno"
                     /locus_tag="VF752_04800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX8854899.1"
                     /translation="MSAIERVHARQILDSRGNPTVEVDVFLESGASGRAAVPSGASTG
                     EFEAVELRDGGSEWGGKGVSRAVANVNGEIGSAVTGLDAADQARLDRTMIELDGTPNK
                     GRMGANAILGVSLATAKATAAEAGQPLWRHLGGDDANVLPVPMMNVLNGGAHADNKVD
                     FQEFMVVPVGFSSFSDALRAGAEVFHALKKTLHDRGLSTAVGDEGGFAPDLDSNEAAL
                     EALIEGISAAGYRPGDDVAIALDPATSEIFEGGSYVLSHEGRELSSEELAAYWADMAD
                     RYPIVSIEDGMDEEDWEGWKLLTERIGDAVQLVGDDLFVTNSERLQRGIEAGVANSIL
                     IKVNQIGTLTETLDAIRIARDAGYTAVMSHRSGETEDVTIADLAVATACGQIKTGAPS
                     RSDRTAKYNQLLRIEEALGGQATYPGRAAFRR"
     gene            complement(9051..9755)
                     /locus_tag="VF752_04805"
     CDS             complement(9051..9755)
                     /locus_tag="VF752_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEX8854900.1"
                     /translation="MSSRSRELERALVALDEITRRLRAECPWDREQDERTIVPHTVEE
                     AYELADAAHSRDDAKMLDELGDVLFQVYFLSLLLEERGKGDLAEVAEHCRQKLIRRHP
                     HVFGDTEAEDAATVLRNWDEIKRTEEGRGAGELFGDVPENLPSTLYARKLLRRASSGG
                     AYSQPSREELAESVRTASSRAERASESDERFDAVGELLFAAVALAREEGVDPELALRR
                     AADAWRQRLERSEVKR"
     gene            complement(9768..10997)
                     /locus_tag="VF752_04810"
     CDS             complement(9768..10997)
                     /locus_tag="VF752_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8854901.1"
                     /translation="MTHILRNALARLRAGRGRAIFLAVPVIVAAVAVAGCGDSVPSNG
                     VAKVGDSVIKKTQFTHWLNAAAAQQQPDPASEIGKLPTPVPPDFKACVAAAESKTVTQ
                     GKQKTKLTPDQAKRVCQEQYNSLRDQVMQFLIQSQWLTQEAKAKNVKVTDAAVQKQFQ
                     DQKKQSFPDDKAYQKFLSSSGMTQQDLLFRVRLDMLTNQLRQKIIEGKGKVTDAQITG
                     YYNKNKSRFSQPERRDLEVVLTKSKAKAAAAKKALQSGQSFKSVAKKYSIDQASKSQG
                     GKLPGVVKGQQEAAFDKAIFSAKKGRLVGPVTTQFGYYVFKVDKVYPQSQQTLDQSKD
                     TIRNLLRSQQEQKALADFVKDYQKKYKDKTNCAKGYVVTTCKNAPKPKSNNKASGGNP
                     QGQPTPSNPQGGSSTNP"
     gene            complement(11123..>13794)
                     /gene="mfd"
                     /locus_tag="VF752_04815"
     CDS             complement(11123..>13794)
                     /gene="mfd"
                     /locus_tag="VF752_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX8854902.1"
                     /translation="PEHRELAEIAATESDEDRPDVAELLPVDRFCDPLSLIPPGALVA
                     VAAEEEIAPTLAALWEDVTTSFHDTDAHHLYLDPDALESELDARAAVKLSAISGDQPF
                     QFRAQGADTSARSLREAEPELEKLVRSGYRTFVAWARQGEAERAAYNLERVQARFLDS
                     QPAPNEPGVLFTTASLRDGFLAPSLKLAVLPEHRLLRRRRAARPEGPRTGAGAIASFT
                     DLRAGDAVVHEDHGIARFTGFETKTVGGVTRDYLELEYRDGDRVFVPSDQLHKISRYV
                     GADAKDPPLSKLGGKQWEQMKIRARRAAQALAGELINLYAERRRRAGHAFELDGEWQR
                     EFEDKFPYRETPDQLDAIEQVKADMEEARPMDRLICGDVGYGKTEVALRAAFKAVQDS
                     KQVMFLVPTTVLAQQHFGTFTERLRDFPIRIEMASRFRSAKQVNETMRDYAEGKVDIL
                     IGTHRLLSRDVRPKDLGLLIVDEEQRFGVKQKELLRQLKLKVDVLSLSATPIPRTLQM
                     SLAGLRDISVIETPPEGRRPVQTYVGEYDEELVKQAIERERARGGQTFFLHNRVETID
                     ETAERVRAICPEARVLVAHGQMEEKQLENVMLSFLRGDGDVLVCTTIVESGLDIANAN
                     TLIVERADALGLAQLYQIRGRVGRSRERAYAYLFYPSSAALTEDAQKRLSTLSDYTEL
                     GSGFKIAMRDLEIRGAGNLLGDEQSGHVAAVGFELYVSMLDEAVALLSGTAADEPPEP
                     VRLDVPVDAYVPGDYVPYEAAKIEIHRRVSGAREVAQLIVLREELEDRFGPIPDPLEN
                     LIKLQDARIKLGRAGARTVGFAAGKLRVSPIELDSRGAKALRAELPEALYESGRSTVS
                     VRVPDDPAARFPAVVAAAEAILKVATEPAPVED"
BASE COUNT         2009 a         4780 c         4806 g         2199 t
ORIGIN      
        1 gttccggtgc ccgacgtgcg cttctccccg ccgccgcagc cggccaccag gagaagcgcc
       61 atcagcgcga tcgggctata ccgcatgcgt ctcaccgggc tggaggatca caacgcggga
      121 ggacgtgttc gcctccacgt cgtccttgaa cgcctgggcg tcggtctcga tcagcgggaa
      181 cgtgtcgtag tggcagggga tgaccgtgtt cgcgcctacg aggcccgcgg caagcgcggc
      241 gtcctggcgg tccatcgtgt agtggccacc gatcgggatg agcgcgacgg cgatgtcgtc
      301 gcgcctgccc gggagcgcca ggtcggagaa cagtgaggtg tcgccaaggt ggtagacgac
      361 cgtgccgttc aggttgatca cgagccctgc cgccggcgaa accgtcccgt tcggggtggt
      421 ggaagtgtgg aacgccggga cgatgcgcac ccagccgccg tcgaacggcg ccgtgccgcc
      481 gaagttcggg ttggtcacct gctcgacgcc ctggctctcc aggtccccgg caatgtccgt
      541 gaccgaaacg cagtgagcgc ccgtccgctt ggcgatctcg acgatgtcgc cgtagtgatc
      601 ctggtgcccg tgcgtgagga agatgtgcgt gggctcgagc tcgtccgggc tcgcggcggc
      661 ctttgggttg ccggtgagga acgggtcgat caggatccgt gtgtcgccgt ccgccagctc
      721 gaagcaggcg tggccgagga agcggatgtc catcgggtca gtcctttctt tggtccttcg
      781 ggatctcgag ctcgcgcgcc agctcccagc cgaccgccac gcccacgagc atcgcgaggc
      841 gcgtctcctc cgcgaggagc gtgcgcacgg cgatcatccg ctcgtgggga tcgtcgcttt
      901 gaagcgcctt cgcgacctcg ctctcgtgcg cctcgagcca gccccccgag tgcagtgcgg
      961 acgcgagtat gcgttgaagc tggggtgcga tcttggcgac gcgggcctcc gcgtcactga
     1021 accgctcggg gtcggtgagc gcctcgagcg cgtcgtcaac ctcggcgtcc gtgaactggc
     1081 gctcgcgcac ggccgcccat aataggtacg tgcaggagct caagctcacc gcgctctcgc
     1141 acggcgccgg ctgcgcgtgc aagctggcgc cgggcgagct tcacgcgctg ctcgaggaga
     1201 tcccgcgtcc cgccgacgcg gaccttcttg tgggctcgga gacggccgac gacgccgccg
     1261 tctaccgcgt cgcgcccgat ctcgcgatcg tcacgacgat cgacttcttc acgccaatcg
     1321 tggacgaccc gtacgaattc ggccggatcg cggccgccaa cgcgctctcg gacatctacg
     1381 ccatgggcgg cgagccgatg accgcgctga acgtggtggc gtggtcgctc gaggagctag
     1441 gccgggaggc gctctcggag gtgctacgcg gcggcgcaga caccgcggct caggccggag
     1501 tggcgatcgt gggcggacac tcgatcgacg atcgcgagcc gaagtatggc cttgcggtca
     1561 ccgggcgcgt gcaccccgac cgcctctacc gcaactccac cgcccgcgcg ggcgacgacc
     1621 tcttcctcac gaagccgctc ggcggcggcg tggcgtccac ggcggcgaag cgcggcgtgg
     1681 cctcgcccga gctgtcacgg cgcgcggccg aggtgatgat ggagctgaac cgtgaggcga
     1741 cgctgcttgc gcgcgaggcc gacgtgtcgg cggtcaccga tgtgacgggc ttcgggctgc
     1801 tcggccacct gcacgagatg acgatcgcaa gcgggctcgc ggccgaggtg gacgcgcgcg
     1861 cggtgccggc gatcgacgga gtgctcgagc tgctgcgctc cgacgagcct ccgatcgcgg
     1921 gcggtacccg ccgcaaccgc gcctgggtcg agccgcacgt gagcttcaac agcaacgtgc
     1981 cggccgagct ggcgtggctc gtgtgcgacg cgatgacgtc cggcgggctc ctgatcgccg
     2041 cgcgcgtggg ctcgggcgcc cccgggaccc ggatcggccg ccttgtggag ggcgcagccg
     2101 gctcgatcgc ggtcagcgct tgagctgcgc gagcccgagg ccgaagaccg cgccggccgc
     2161 ggcaccgccg acgccggcga agatgctcgc gtcgggccac gccaccgcga ccagcagcag
     2221 caccgccgcg aacacgtagg cgccgatcag gtcgctctca cggtcgtcgc cgcggccgag
     2281 cgccatccgg tcgtccacga gccatgccgt cacgagcccc agcgccgcgc cgttcgcacc
     2341 cagcgccggc aggtcgttca gcgcaaccgc gagcgccgcg cccgcagcgc cggagccgat
     2401 gaagacgaac aggggcgccc accaaccgaa gcggcgctcg agcgagctgc cgaacagccc
     2461 tgtggcgaac agtgcgatga acgcgtagcc cgcgttgttg tgcaggaacg gcgtggtgag
     2521 cacgcgccac cactcgtcgc ccggcttgat gaagagaccg cccatgtcgt cgatcgacag
     2581 gtcggtggag ctcgagatga caccggacac ggcgaccagc gtcgcgacga gcgaggcgac
     2641 gacaaggagc gcggtcgcgt agggccgagc ctcgatcgcg atgccaggaa tctcgccccg
     2701 ccgcatgcgc ggaagcgtcg gccggcggcg cctgcgctgc ggctcctcgc cgcgcctgcc
     2761 gcgctcgatc ttcggcgccc gcttgcgcac ccgctggccg cagtacgggc actcggtcac
     2821 gtacgggctc acctccgagc cacagttgcg gcaaacgacg aagaggtccg gctggctcac
     2881 tggtccgagg atactggcga cggccagtac tactgccgga ttcttaggtc ccggggctag
     2941 accggggcgg aatccggggc ggtcttctcg acgatctcgg cgacgacctc cttgaggtcg
     3001 tggttggcct cgtagaccac cacctggcgg gaggcgccgt tgccgcggtc gagcaggtcc
     3061 tcaatgcagg cgagatcgtc agccgagccg aggtcctgcg cgtgctcgcg catgcggtga
     3121 agcacccggc gaaccagcgc gcgcgtctgc acccgacccg tcttcggcag gtccaccagc
     3181 tctccgtcga gcccgtgccg ggcggccagc catttattct catcgagcat ctggtacggg
     3241 aactgcgaca gccgtttgcc ggcgtcgaag tgctcggcca gctcgcgcac gagcgcctgg
     3301 atgagcgccg cgatggcgag cgtgtgctcc acgcgggtct gagcgtccat gacgcggatc
     3361 tcgaccgtgc cgaagcgggg gtgcgggcgc acgtcgtacc agaggtaggt gtagtcctcg
     3421 atcaccttcg agtcgaccat gaactgcacc cgcttggcga agtcctcgaa gtccttgtat
     3481 gtgggcggaa tgccgacgcg cgggaaggcg cggaagatcg gcgtgcgcgt ggagaggagg
     3541 ccggtcgagt ccgcgcgcca gaacggtgag ttcgccgaca gcgccagcag catcggcagg
     3601 tgcacgcgca tgccgttggc cacgtggatc gccttgtccg ggtcgtccac cgccacgtgc
     3661 acgtgcgcgc cgaagatcag ctcctggcgc gccacgaacc ggagcgcggc gatcaggtcg
     3721 cggtagcggc tgcgcgcgac cacccgctgg tcctcccaca tcgcgaacgg atgggtgccc
     3781 gacgagccga tggccagccc gtactcggcg gctgtcctcg aaacctctcg ccgaagcgcg
     3841 cgcagctcct cgccggcgtc gcgcgtgttc ttgtggggct gagtggcaac ctccagcacc
     3901 gactcgagca gctccggctt cacctgctcc tcgcgctgag gcaccgcgcg cagcatcgtc
     3961 tcgatcgagt tcgagagatc gagcgtctcc gcgtcgagga tcatcagctc ctcctcgatc
     4021 ccgatcgtgt aggagggccc cgtgaatcgg tgctccacgc gcgcatcgta cccgcgtgcg
     4081 gggtgggtac ccacttctta gcgctctcca tttgcgcttg aatgatccgt accccatcga
     4141 cctattagga ggcgaaggca aagccccatg acaccgggat atgacggaaa gctgttcatc
     4201 ctcgcgttcg atcaccgcgg ctcgttccag aagaagtggt tcgggatcga gggtgagccg
     4261 agcgaagagg acgtgaaccg aatcgcggac gcgaagcacc tcgtgttcga gggcctgctc
     4321 gccgcgctcg agcagggcgc cgaggcgagc gtgaccggcg cgctcgtgga cgagcagttc
     4381 ggcgggccga ggaacatccc gcaggaggcg aagcagcgcg gtctcaaggt cgcgatgccc
     4441 gtcgagaaga gcggccagaa cgagttcgac ttcgagtacg gcgaccagtt cggcgagcac
     4501 atcgagaagt tcgaccccga cttctccaag gtcctcgttc gctacaaccc cgacgacgat
     4561 ccggacatga accgccagca gctcgagcgt ctgaagaagc ttgcggactg gctgcacgag
     4621 cgcgatcgcc tgttcctgtt cgagctgctc gtgccggcca ccgaccagca gctcgagtcc
     4681 gctggaggcg actccgagcg ttacgacgcc gagctgcggc cggagctgat gcgccgggcg
     4741 atcgaggaga tccaggactt cgggatcgag gtggacatct ggaagatcga gggcgtggac
     4801 gagcgctccg acgccgagat gctcgcgcag cagacccgtg ccggcggtcg cgacaacgtg
     4861 gtgtgcgtgc tgctcggccg cggcgccaac gacgacaagg tcgaccactg gctgcgccag
     4921 gccgcgcccg tcgagggctt cgtcggcttc gcgatcggcc gctcgatctg gggtgacccg
     4981 ctgaaggcgt tcatcgacgg ctcgctcgag cgcgaggcag ctgccaagca gatcgcggac
     5041 aactatctgc gcttcgtgcg cgtgtacgag gaggccgagg cggcaacgcc cgcctagggt
     5101 gccccgtcag ggctgcgcga gcgataccct tccctgccgt ggcgactcgc atgcgccaat
     5161 cgctcgcgca gttcgaggcg gccttccgtg aggaggcggc cgaagagcgg ctgcgccggg
     5221 agcggctccg ccgcgaggcc gctgaacgct cgcgcgctcg ccgccaccag aaggtccaga
     5281 agagcggcac catgcgcttc ggcgtgctgg tgttcgtgat cctcgcgacg acggtgatcg
     5341 tgacgatcgc gatgttccag acgctctcgc tgctgatcgg cggctaggcc gcccgcgctc
     5401 gcgtcggcgc gtcgtccgca gccgctactt gccggactgc gaggacttca ggcgttcgag
     5461 cgcctcgttc acctcggcac ggtcggcgtg ctctgcgccg gcggccttct cctgctggat
     5521 ctcgaggtag acctctttac ggaagaccgg gacggagcgc ggcgcctcga tgccgatgcg
     5581 gaccttctcg cccatgatcg cgagtaccga gacctcgatg tcgtctccga tcatgatgct
     5641 ctggttcgcc tttctcgtca ggaccagcat caatgcctcc ctgccccagt acatgggaca
     5701 tgcctgcgtc cgtcgagcac accaccggtc aagggcccgg tatgagcgcc tccgtccgtg
     5761 agtgtatcgg tcatcgtcac cgcgccctgc aggtcctttt gagggcagcc cacgagcctt
     5821 ctccctcgtg acggggagcc cagagtctag gccaacgctg ggatgcgatg gaagaggtct
     5881 ggacgtttgt cgtgggggaa tcagcctgct ctctgcgatt gctagtgttg ccgccgctcg
     5941 tggctagacg atcgctcaaa tcgcagctca atcaaatccg cagctgggtc cgcgaggggc
     6001 gtacggacgc ctggatcgcg caccagctgg aggtcagcgt gaaggacatc gacgccttca
     6061 agcgcgagca cgggctggct cctgcgggcg aggatggcaa cggcgacggc ccccgctacg
     6121 acgacgaggt ggacctgcgc gccgaggacg acgcgctcgt ggccgccgag ctggaggccg
     6181 agcccgacga ggacgagctc gaggaggacg aggaggagct cgaggaggag ctcgaggagg
     6241 gcgagcgccc gacccgccgc cgccgcgggc gtcgtggcgg acgcggccgc aagcgccgcg
     6301 ccgggccgcg cgagctcgag ggcactttcg atcacgggga cgagggctat ggcctgtggc
     6361 tggaccccga ggtccaggac gacgcgatct acgccgagca ctgggccggc catcgccctg
     6421 tgactgtcac gatcgagccc aaccggatcg tcatccgccg cgccggcgag gacgtcggcg
     6481 acaacgacga agacgagaac gacgaggact agcgctcagt cgcgctcgcc cggcgaggat
     6541 ccagcgctca gctacgcctg gcaggccggt cgccagccgt agctggaccg gagtccgggc
     6601 gcttaatctc ctcgcgggcg cgtccgcctg cgtagttcgt ggcctcccgc gcgtagcggt
     6661 ggaaggctga gtccaccacc caggttgtgt ccgtgcccga ggcccagcgc tgggcgattt
     6721 cgtccgccca gtccgtgccg cccgcgtaga agtccgccag ctcctcgacg ctgaacgagg
     6781 acccgagcct gcgccggagc tcttcgagga ccgcgtcggc ggcgcgctcg agctggaagc
     6841 gcgcgggctc gtcggtgtcg cggacgcgac gctcgccctc gcgccactga aacagggcgt
     6901 tctcgacggg atagggcact cccccacggt acccagggcg tccagcccgg aacgccttcg
     6961 gtgcttagcg acccgggatg ttctcgatca cgtaggcccg ctcgccctgc ttcaccatgc
     7021 cgagctcgcg cgcgcggcgc tcgagcgact gagggctccg cagatccgcg attccctgct
     7081 tgaggcgggc gttctcctgc tcgagcgaac gcagctcctc gcgctggcgg ccggccgtct
     7141 gcgactgcga gaagtagcgg tgaagcggcg agatgtagag gaggaggatc acgccgagga
     7201 ccgccaggag cgacatgcgg cccacgcggt cccagcggat ccccgatccg ccggtgcgcc
     7261 gcgagccggg cggggcgggg cgcctgcgcc gggcagcggc ggcacgggag cgggcaacag
     7321 tcgcttgggc catttgggcg gagttcgcgc cgcctagcgc catccctgcg gtcggccagc
     7381 aggtgggcgc cacctttcat ccgctttttc ggagttgcgc agcgcaacga tcggggttgc
     7441 gcgccgccac gatccgagtt gcgcgccgca actctgcaaa acgatgggcg ccgtccggcg
     7501 agccccggcc acccggctgg tcgaccgccg gaccagccct tcccttttga tcgttgaacg
     7561 aatcaacgat cgcgaggcga ggcgcgggcc gggctgcccg agtcgccggc gtactcgatc
     7621 agggcggggt cccgcagcgt cgcgtcgccg gagaagggtc ggcgcaatcg ttgaatcgat
     7681 caacgatgtt ttggacggcc gggtgctgag ggtcggcccg acgaccggtg ccgagggtcg
     7741 gcccgacgac ccctcaggcc ggagggctca tcgcgccgcc tagcgcctga aggctgcgcg
     7801 gcccgggtag gtcgcctggc cgcccagcgc ctcctcgatc cgcagcagct ggttgtactt
     7861 cgccgtgcgg tccgagcgcg acggcgcccc ggtcttgatc tggccgcatg ccgtggccac
     7921 ggcgaggtcg gcgatcgtca cgtcctcggt ctcgcccgag cggtgcgaca tcaccgcggt
     7981 gtagccggcg tcgcgggcga tccggatcgc gtcgagggtc tcggtgagcg tcccgatctg
     8041 gttcaccttg atgaggatcg agttcgcgac gcccgcctcg atcccgcgct gcaaccgctc
     8101 gctgttggtc acgaacaggt cgtcgcccac cagctggacc gcgtcgccga tccgctccgt
     8161 gagcagcttc cagccctccc agtcctcctc atccatgccg tcctcgatcg acacgatcgg
     8221 gtagcgatcg gccatgtccg cccagtaggc cgccagctcc tcgctggaca gctctcggcc
     8281 ctcgtgcgag agcacgtacg agccgccctc gaagatctcg ctggtggccg ggtcgagcgc
     8341 gatcgcgacg tcgtcgccgg gccggtagcc cgccgctgag atgccctcga tcagagcctc
     8401 gagcgccgcc tcgttggagt cgaggtcggg cgcgaagccg ccctcgtcgc cgacggcggt
     8461 ggacaggccg cggtcgtgca gcgtcttctt gagcgcgtgg aagacctcgg ctccggcgcg
     8521 cagcgcgtcg gagaacgacg agaagccgac cggcacgacc atgaactcct ggaagtcgac
     8581 cttgttgtcc gcgtgagcgc cgccgttgag gacgttcatc atcggcaccg gcaggacgtt
     8641 cgcgtcgtcg ccgcccaaat gccgccagag cggctgcccg gcctcggcgg cagttgcctt
     8701 cgccgttgcg agcgacacgc ccaggatggc gttcgcgccc atgcggccct tgttgggcgt
     8761 gccgtcgagc tcgatcatcg tgcggtcgag gcgggcctgg tcggcggcgt cgaggccggt
     8821 cacagcggac ccgatctcgc cgttcacgtt ggcgacggcg cgcgagacgc ccttgccgcc
     8881 ccactcggag ccgccgtcgc gcagctccac agcctcgaac tcgccggtgg aagcgcctga
     8941 cggaacggcg gcccgtccgg acgccccgga ctccaggaac acgtcgacct cgacggtcgg
     9001 gttgccgcgg ctgtcgagta tctgccgcgc gtgcacgcgc tcgatcgcgc tcaacgcttc
     9061 acctccgagc gctccagtcg ctgtctccat gcgtcggctg cccggcggag cgcgagctcc
     9121 gggtcgacgc cctcctcgcg cgcgagcgct accgcggcga acagcagctc gccgacagcg
     9181 tcgaagcgct cgtcgctttc ggaagccctc tcggcgcgcg aggacgccgt ccgcacggac
     9241 tccgcgagct cctctcgcga gggctgcgag tacgcgccgc cggaagatgc ccggcgcagg
     9301 agcttgcgcg cgtacagcgt cgacggcagg ttctcgggca cgtccccgaa cagctcacct
     9361 gccccgcggc cctcctcggt gcgcttgatc tcgtcccagt tgcggagcac ggtcgccgcg
     9421 tcctcggcct ccgtgtcgcc gaacacatgc ggatgacggc ggatcagctt ctgcctacag
     9481 tgctcggcca cttcggccag gtcgcccttg ccgcgctcct cgagcagcag cgagaggaaa
     9541 tacacctgga acagcacgtc gccgagctcg tcgagcatct tcgcgtcgtc gcgcgaatgc
     9601 gcggcgtcgg ccagctcgta ggcctcctcg acggtgtgcg ggacgatcgt gcgctcgtcc
     9661 tgctcacggt cccaggggca ctccgcccgc agccggcggg tgatctcgtc gagcgcgacc
     9721 agcgcccgct cgagctcacg gcttcggctg ctcatcggag cgagagctta cggattggtc
     9781 gacgagccgc cctgcggatt cgacggcgtg ggctggccct gcgggttgcc gccggaggcc
     9841 ttgttgttgc tcttcggctt cggggcgttc ttgcacgtgg tgaccacgta ccccttcgcg
     9901 cagttcgtct tgtccttgta cttcttctgg tagtccttca cgaagtccgc gagcgccttc
     9961 tgctcctgct gcgagcggag caggttgcgg atcgtgtcct tggactggtc gagcgtctgc
    10021 tggctctgcg ggtagacctt gtcgaccttg aagacgtagt agccgaactg cgtcgtcacc
    10081 ggcccgacga gccggccctt cttcgcgctg aagatcgcct tgtcgaacgc ggcctcctgc
    10141 tgtcccttca cgacgccggg caacttgccg ccctgcgact tcgaggcctg gtcgatggag
    10201 tacttcttcg ccaccgactt gaagctctgg ccgctctgca gcgccttctt cgccgccgcc
    10261 gccttggcct tcgacttggt gagcaccacc tcgagatcgc ggcgctcggg ctgcgagaag
    10321 cgtgacttgt tcttgttgta gtagcccgtg atctgggcgt cggtgacctt gcccttgccc
    10381 tcgatgatct tctggcggag ctggttcgtg agcatgtcca gcctcacccg gaagagcagg
    10441 tcctgctgcg tcatgcccga cgaggagagg aacttctggt acgccttgtc gtccgggaac
    10501 gactgcttct tctggtcctg gaactgcttc tggaccgcgg cgtcggtgac cttcacgttc
    10561 ttggccttgg cctcctgcgt cagccactgc gactggatca ggaactgcat tacctgatcg
    10621 cgcaggctgt tgtactgctc ctggcagacc cgcttggcct ggtcgggcgt gagcttggtc
    10681 ttctgcttgc cctgcgtgac ggtcttcgac tccgctgcgg ccacgcacgc cttgaagtcc
    10741 ggcgggaccg gcgtggggag cttgccgatc tccgaagccg gatccggctg ctgctgcgcc
    10801 gctgcggcgt tcagccagtg ggtgaactgg gtcttcttga tgacggaatc gcccaccttg
    10861 gcgacgccgt tcgagggcac gctgtccccg cacccggcga ccgcgactgc ggcgacgatc
    10921 acggggacgg cgagaaagat cgcacggccg cggccagcgc gcagacgagc aagagcatta
    10981 cgcaggatgt gggtcattcg agagtgtccg gaagactgtc gggggcctgt agggcgatgg
    11041 gctagtagat acgccggtat tcaccgcccg gctcagccac tgaaacggcg gaccatccta
    11101 cctgagcact ccccacagat gctcagtctt cgacgggcgc cggctcggtg gcgaccttca
    11161 ggatagcctc ggctgcggcg acgacggccg ggaagcgcgc cgcggggtcg tccggcaccc
    11221 gcacgctcac tgtgctccgg ccggactcgt agagcgcctc gggcagctcc gcacgcagcg
    11281 ccttggcccc gcgggagtcg agctcgatcg gcgacacgcg cagcttgccc gcggcgaagc
    11341 cgaccgtacg cgcgcccgcg cggccgagct tgatgcgcgc gtcctggagc ttgatcaggt
    11401 tctcgagcgg gtccggaatc gggccgaagc ggtcctcgag ctcctcgcgc agcacgatca
    11461 gctgggcaac ctcgcgggcg cccgacaccc gccggtggat ctcgatcttc gccgcctcgt
    11521 agggcacgta gtcgcccggc acgtaggcgt cgaccggcac gtccagacgc accggctcgg
    11581 gcggctcgtc cgccgcggtg ccggacagca gggccacggc ctcgtcgagc atcgacacgt
    11641 agagctcgaa gccgaccgcc gccacgtggc cggactgctc gtcgccaagc aggttgccgg
    11701 cgccgcggat ctcgaggtcg cgcatcgcga tcttgaagcc cgagccgagc tcggtgtagt
    11761 ccgagagcgt cgacagccgc ttctgcgcgt cctcggtgag cgccgcggac gacgggtaga
    11821 agagatacgc gtaggcgcgc tcgcgggagc ggccgacgcg gccgcggatc tggtagagct
    11881 gcgcgagccc gagagcgtcg gcgcgctcga cgatcagcgt gttggcgttc gcgatgtcga
    11941 gcccggactc gacgatcgtg gtgcacacga gcacgtcgcc gtcgccgcgc aggaacgaga
    12001 gcatcacgtt ctcgagctgc ttctcctcca tctgcccatg cgccacgaga acgcgcgcct
    12061 ccgggcatat cgcccggacg cgctcggcgg tctcgtcgat cgtctcgacg cggttgtgga
    12121 ggaagaacgt ctgcccgcca cgggcacgct cgcgctcgat cgcctgcttc acgagctcct
    12181 cgtcgtactc gcccacgtag gtctgcaccg ggcggcgccc ctcgggcggc gtctcgatca
    12241 ccgagatgtc gcgcaggccg gccagcgaca tctgcagcgt gcgcgggatc ggcgtggccg
    12301 acagcgacag cacgtcgacc ttcagcttca gctggcgcag gagctccttc tgcttcacac
    12361 cgaagcgctg ctcctcgtcc acgatcagca ggccgaggtc cttcggccgc acgtcgcgag
    12421 agaggaggcg gtgagtgccg atcaggatgt ccaccttgcc ctcggcgtag tcgcgcatgg
    12481 tttcgttcac ctgcttcgca gagcggaagc gcgaggccat ctcgatccgg atcgggaagt
    12541 cgcggaggcg ctcggtgaag gtgccgaagt gctgctgggc gagcaccgtg gtcggcacca
    12601 ggaacatcac ctgcttgctg tcctgcacgg ccttgaacgc cgcgcgcagc gccacctcgg
    12661 tcttgccgta gcccacgtcg ccgcagatca ggcggtccat ggggcgcgcc tcctccatgt
    12721 cggccttcac ctgctcgatc gcgtcgagct ggtcgggcgt ctcgcggtag gggaacttgt
    12781 cctcgaactc gcgctgccac tcgccgtcca gctcgaaggc gtggcccgcg cggcggcgac
    12841 gttcggcgta gaggttgatc agctcgccag cgagcgcctg cgcggcgcgg cgggcgcgga
    12901 tcttcatctg ctcccactgt ttgccgccga gcttcgagag cggcgggtcc ttggcgtccg
    12961 cgcccacgta gcggctgatc ttgtggagct ggtcgctcgg cacgaacacg cggtcaccgt
    13021 cgcggtactc gagctcgagg tagtcgcgcg tcacgccgcc gaccgtcttc gtctcgaagc
    13081 cggtaaagcg cgcgatgccg tggtcttcgt gcaccaccgc gtcgccagcg cgcaggtcgg
    13141 tgaaagaggc gatcgcgccc gcgccggtcc gcgggccctc gggccgcgcc gcccggcggc
    13201 gccgcagcag ccggtgctca ggcagcaccg cgagcttcag cgagggcgcg aggaagccgt
    13261 cccgcaggct cgccgtggtg aagagaacgc ccggctcgtt cggcgccggc tggctgtcga
    13321 ggaagcgcgc ctggacgcgc tcgaggttgt aggccgcgcg ctcggcctcg ccctgccggg
    13381 cccaggcgac gaaggtgcga taacccgagc gcaccagctt ctcgagctcc ggctcagcct
    13441 cccgcagcga ccgcgccgac gtgtccgcgc cctgcgcgcg gaactggaac ggctggtcgc
    13501 ccgagatcgc cgacagcttc accgcggcgc gcgcgtcgag ctcgctctcg agcgcgtccg
    13561 ggtccaggta caggtgatgg gcgtcggtgt cgtggaagct cgtggtcacg tcctcccaca
    13621 gcgcggccag cgtgggcgcg atctcctcct ccgcggcgac cgccacgagc gcgcccggcg
    13681 ggatgagcga gagcgggtcg cagaagcggt cgaccggcag cagctccgcc acgtcgggac
    13741 ggtcctcgtc ggactccgtc gcagcgatct ccgccagctc gcggtgctcc ggcg
//
LOCUS       DASVXS010000081        14965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_446316, whole genome shotgun sequence.
ACCESSION   DASVXS010000081 DASVXS010000000
VERSION     DASVXS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446316"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VF752_04820"
     CDS             <1..1022
                     /locus_tag="VF752_04820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX8854903.1"
                     /translation="TLRRNITEERLREGAHDERHLELLRAMGTKSLMAVPMPAGTRPV
                     GVLSFGTTESHRQFDENDLALAQELGRRAGLAVENARLYTERTQIARTLQHELLPPAL
                     PSMPGWEIEAHYQPAGEENEVGGDFYDAFPVADGWMVVIGDVAGRGIEAASLTALARY
                     TLRAAGTLLGDPVAAVSELNRELRERGDLALCSVACAMLTRSPAAASATIVCAGHPLP
                     FLLRGGVPEQVGSFGTFLGVGDEANWGVVELTLEPGDQLLLYTDGVIDARGLEERFGE
                     ERLMETLTGARDPAGAVEAVVAALAAFQAGSQADDTALVGIRLAPPEGVRHPQENPVE
                     TLSDA"
     gene            complement(1288..2355)
                     /locus_tag="VF752_04825"
     CDS             complement(1288..2355)
                     /locus_tag="VF752_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8854904.1"
                     /translation="MARDRRQALIALAAAGIGWGLTIPFSKVALAWLGPLSLGVARFA
                     IAAPVLALAGRRGLRGALSWRVIGWGALFYGGVVGLQNTGIALTSVTHAALIIGAVPA
                     LVALATLARGEGRSGRVEWTGFAAAIAGAGLVAGSGGTASTGGDALMLASAALSALFV
                     AAQPAVLRGRDPVAVTAVQMAVGVIVTLPFALAFEGVPRTLPSGDVLTAFAALVLVGS
                     LLPFALYAYGQARVAAEVAGAFLNLETVVGAAVGAVAFGDPFSRSQLLGAAAIVGGLL
                     LSVAPARGRAADSGDLHLEPNAFAPTASGNSPAARKPVEDDESVPTAACDARARLPRR
                     VKARAGVPDLHPHALRCNAEC"
     gene            2438..2944
                     /locus_tag="VF752_04830"
     CDS             2438..2944
                     /locus_tag="VF752_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8854905.1"
                     /translation="MAITLDRIDVTLLRELQTDAGRPNVELAKQVGLSPAATLNRVRR
                     LRDAGILGPARARVDANAAGFPLLVYVLATLAEHDEAAHRRFKAAVDKIPNVVSADWV
                     TGETDALLQVVARDVDELQRVLLLLSSRAGAQRLVTLLRLEELKPAAPLPVAPASLSE
                     RAAAREGA"
     gene            3018..3593
                     /locus_tag="VF752_04835"
     CDS             3018..3593
                     /locus_tag="VF752_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854906.1"
                     /translation="MADVASPAARARTGPDDQGAGEKAKETAQQAAGQAQEKAQEVAG
                     QARGRVREQVDQRSSQVGRQLSQSAQDARSIGEQLRRQGKDGPARLADQAANRAERVG
                     GYLENSDGDKLLRDVEDFGRRQPVAVVLGGLVIGFAASRFLKASSEDRYRSTSVDRYP
                     STPRPSTGRESFGGTVPPSPALGETGLPRTS"
     gene            3602..4027
                     /locus_tag="VF752_04840"
     CDS             3602..4027
                     /locus_tag="VF752_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8854907.1"
                     /translation="MTTTDENQAELRERPIGELLRQLSQETTQLVRQEIELAKAEVSE
                     KGKKAGQGAGMFGGAGVAGLLALGALTACLIVALDLAMPTWLAALIVTLIWGAVAGVL
                     ALTGRERVREAAPAVPEQTVETLKEDVQWAKTQTPSARR"
     gene            3991..4515
                     /locus_tag="VF752_04845"
     CDS             3991..4515
                     /locus_tag="VF752_04845"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX8854908.1"
                     /translation="MGQDPDAIRAQIEETRGQMGETAQAIGYKADVKERTKESVQEKT
                     DRLKSKITGAGSRLNEATPEGSEVKQTAQRAAGIAQQNPIGLAIGAVAVGFLAGLALP
                     RTRMENERLGPIADDVKERAKETGQEALERGKQVAQDAAQSAAEAAKESGREQSEGLQ
                     QSAQESAQEVRTRS"
     gene            complement(4571..4852)
                     /locus_tag="VF752_04850"
     CDS             complement(4571..4852)
                     /locus_tag="VF752_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854909.1"
                     /translation="MTCERAQAYGRVMKMIENLGPSKLQPSELDSIRETADQLLFTED
                     IDTAESALTDAIELAVRLTDSGRWIDETANQLVAELAACGPVELRPVAT"
     gene            complement(5139..6929)
                     /locus_tag="VF752_04855"
     CDS             complement(5139..6929)
                     /locus_tag="VF752_04855"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8854910.1"
                     /translation="MHLPRCVSAFAATLFLSAALTSSAFAAGPAPFGHACISQGGARF
                     CPTTDLGQRVPTFDGVPIDVDVTLPPSGNGPYPALVLMHGYGGSKAAYEAADANDPKK
                     RFANNLYYAQRGYAVITLSARGFGRSCGSVASRTPDCAKGWIHLGDQRYEARDTQLLL
                     GRLVDEGIVDPKGIGVSGGSYGGGQALELGFLKNRVRLTNGKFTAWKSPKGTPLQIAA
                     AAPIIPWSDLASALVPNGRWLDTEVPTSRIDSSPVGVPIISYIDGLYIGGAASGFVAP
                     PGADPRADLANWKAVTDAGDFADKRVPKIVSELHRFHSAISIPGNPAPLLISNGWRDD
                     LFPVGQALRAYNLIRSRHANAPVSLSFLNIGHPPGGNKTVTTEEGAKLISSFFDARLR
                     GKGRAPAPGSVYTGSVTCPQTAPDSGLVRSKSWDAAHPGTFTMSGRKAQTATSDGGNP
                     ATGKAFDPIAGFDNPCKTVAAEKAPGTAVVEAKSPGFTLLGLTTISASIRAQGVFPYL
                     AARLWEVTSGQQLLVDRGVVRLDRNQRGRIRFQLHGTNHRFARGSTVKLELLGRDANY
                     YQVSKVPFSVKLSNVSAELPTRERGERKGR"
     gene            7009..7434
                     /locus_tag="VF752_04860"
     CDS             7009..7434
                     /locus_tag="VF752_04860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8854911.1"
                     /translation="MAARPGYAHVLAIPTRWADNDVYGHVNNVEYYAFFDTVINAWLI
                     REGGLDIHRGEVIGVCAESRCEYKAPLEFPETVEAALRVGQLGRSSVRYEIALFREGA
                     DEPAATGHFVHVFVDREARRPVEIPERLRGALERLVVDA"
     gene            7431..8555
                     /locus_tag="VF752_04865"
     CDS             7431..8555
                     /locus_tag="VF752_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX8854912.1"
                     /translation="MKARGAVLRQMGLPSPYSESRPLELAEVELDPPGEGELLVRVGA
                     AGLCHSDLSVIDGSRPRVMPMLLGHEAAGEVVETGPGSTGFAVGDHVVLAFVPSCGEC
                     GPCRAGRAALCEPGAAANTAGTLLSGAHRIHGENGEVLNHHLGVSCFADHIVVSQHSA
                     VRVDPELPVEVAALFGCAVLTGVGAAVNSAQVGVGDRVAVFGLGGVGLAALLGARVAG
                     AAELIAIDVVPEKLALAAELGATHTLAAGDETVGQIRELTGGGAEKVIETVGNERVLG
                     QAYAATRRGGTTVSVGLPHPDRMLSIPAVSLVAEERTLKGSYLGSCVPERDLPRFIEM
                     YREGRLPVDRLLTHRISLDEVNEGFDRLARGEAVRQAIVF"
     gene            complement(8552..10336)
                     /locus_tag="VF752_04870"
     CDS             complement(8552..10336)
                     /locus_tag="VF752_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8854913.1"
                     /translation="MEADGSKLGLALSGGGFRASFFHIGVLARLAELGVLRRVEVIST
                     VSGGSIVGALWYLHAKKLLEQDADPSPQRYVEEVQALEDEFLEATRRNIRGRVFLNLA
                     KNFYMARLSYSRSDRIGDLYDRYFYKPAWPGERPKRYRLIDKQIEMRELLIQPAGVSE
                     PFDPDVHNAGREAKVPILLLNATSLNTGHNWRFEAVRMGEPMPADDAMREVMSDVDKN
                     ELLGQGYYGELGKPAGQKPIAEKQRDFPLGLAVAASACVPGLFHPLSISGMYRDGRRV
                     QLVDGGVHDNQGVQGLFDRGCTHLIVSDASGQMQEQTQPGARVLSVLGRSTSIYGARL
                     RNEQLVRASREGAAAGHGLRLMHLRAGLGRHVEPPLAEGDEYAYAPSEQAPHVGAEAF
                     GVAKQVQSLLARVRTDLDLFGDSEAFTLSLDGYRMTGAFFPGDGEFAQFVPDAPPGED
                     PARWQFSGVSDLVGPQAARPGWYTRALRAGSKRFLKPLFLSWFLCGAIAALVGVGIWQ
                     LVANWADVTSSLSARPKLWWTLLPLLLLVLYVADKVRMVLVRWPSQLLVSIALPFIFA
                     PLLWLFALVSLPEGWLYRRLARVRPPPR"
     gene            10401..11297
                     /locus_tag="VF752_04875"
     CDS             10401..11297
                     /locus_tag="VF752_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8854914.1"
                     /translation="MATHRQAAVNGIELHVAEEGSGPPVILAHGFPELWYSWRDQLPA
                     LADAGYRVLAPDMRGFGGSSAPADIEAYDMFTIADDLIGLLDDIGEEKAVLVGHDWGA
                     NAVWWTALAHPERVAAVVGMSVPIVPRAPAPPIGLLRQGLSDDFYMIWFQEPGVADEA
                     LSRDVRRTLTTSRVWDANWAERDDDPRRPDWLSEEELAVYVETFTRTGFTGGLNWYRN
                     IDRNWELSEPIADRRVDQPSLFIAGTRDPVLAFMPPEAMDGMVTDLRGKLMIEGAGHW
                     VQQERPDEVNSALLRFLREVGW"
     gene            11331..11453
                     /locus_tag="VF752_04880"
     CDS             11331..11453
                     /locus_tag="VF752_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854915.1"
                     /translation="MTTHQTELDIAMRVLKLVLVMVAVLVGIALLTVAANALFT"
     gene            complement(11800..12432)
                     /locus_tag="VF752_04885"
     CDS             complement(11800..12432)
                     /locus_tag="VF752_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854916.1"
                     /translation="MADWATISALATAGGTLALAAATYGSVRSANRSARTAEIALQEQ
                     RRPVLVQSRLDDAEQKIMFVDRHWARVSGGGGTAEHSDGVLYLVLSLRNVGLGIAVLQ
                     GWHARAGLARPDIAHAELEEMRPQTRDLYIPAGDIGLWQGAFRDQHDAGYPEIARALD
                     DREPISIDLLYTDQIGDQRTISRFVLNPVGDDRWIASVGRHWYLDRAGPR"
     gene            complement(12454..13410)
                     /locus_tag="VF752_04890"
     CDS             complement(12454..13410)
                     /locus_tag="VF752_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8854917.1"
                     /translation="MELRHLRYFVAVAEELHFRRAAERLHVAQPAVSEQVRKLEQELG
                     VQLLERTQRNVSLTSSGAAMLEEARSVLRQAEVARLAARNARDRESARLRIGYVASAL
                     PAMVPRALQNLSSATPQLETALETGLSQDLIEAVRNGRLDAAIVPMPAVADRLKITQL
                     GDQRAVAALPIGHRDAVKGALSLRRLAPERILLLPRDENRPFYDAVVATCHASGLSPT
                     LVELAGTHVEEALLAVASGAGMALLPESVSDRYAAPGVRFLPLDETELSFQTAAITRP
                     DSTHLPTHGLLRALSRAQRPRLDSAPEPEPEPEPQPGVVAIA"
     gene            13682..14080
                     /locus_tag="VF752_04895"
     CDS             13682..14080
                     /locus_tag="VF752_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX8854918.1"
                     /translation="MKARHSILSLIVVAAVTILAAGCGGGSSNANSSNSNTSTGASSQ
                     PAPSSGSNAVKISNFTFVPATLSVKGGASVTVTNNDSTTHTFTSDDGHSFDTGNIGTG
                     SSKTVSVSKAGRYPYHCSIHPFMHGTLVIK"
     gene            14108..14653
                     /locus_tag="VF752_04900"
     CDS             14108..14653
                     /locus_tag="VF752_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX8854919.1"
                     /translation="MSTNGSGTALLVIDMQQEVVDSCPDGTGLINRINSLSRRAAHVG
                     VPVIFIQHEGEDELLKGTPGWEFAAGLERASDSLLVPKTYRDAFANTELGDLLERLGV
                     RRVVVTGAHSDFCVQTTALSALFHGFDLVLVSDGHAAEPSPHEDGLMPEALEAFVNAR
                     FRTLRYPERTVEVVPAADVEL"
     gene            14643..>14965
                     /locus_tag="VF752_04905"
     CDS             14643..>14965
                     /locus_tag="VF752_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854920.1"
                     /translation="MSSRGIQPELWVDRAADAVAFYEAAFGARVVHRVGEGQDIVAQL
                     VVGDDAFWVSAASSEMGRFSPHAIAGATSRTLLVVDDPASVVERAVAAGATEIDIVIT
                     RQHVLT"
BASE COUNT         2386 a         5175 c         5275 g         2129 t
ORIGIN      
        1 ccacgctcag gcgcaacatc accgaggagc gcctgcgcga gggggcccac gacgagcggc
       61 acctcgagct cctgcgggcg atgggcacga agtcgctgat ggccgtgccg atgccggctg
      121 gcacccggcc ggtgggcgtg ctctcgttcg gcaccaccga gtcgcaccgc cagttcgacg
      181 agaacgacct ggcgctcgcg caggagctcg gccgccgcgc cggactagcc gtcgagaacg
      241 cgcgcctcta caccgagcgg acgcagatcg cgagaacgct tcagcacgag cttctgccgc
      301 ccgcgctgcc gtcgatgccc ggctgggaga tcgaggccca ctaccagccg gccggcgagg
      361 agaacgaggt gggcggcgac ttctacgacg ccttcccggt ggccgatggc tggatggtcg
      421 tgatcggcga cgtcgccggg cgcggcatcg aggccgcctc actcaccgcg ctcgcgcgct
      481 acacgctgcg cgccgccgga acgctgctag gcgaccccgt cgccgcggtg tccgagctga
      541 accgcgagct gcgcgagcgc ggcgacctcg cgctgtgcag cgtcgcgtgc gcgatgctca
      601 cccgctcgcc cgcagccgcg agcgccacga tcgtctgcgc cggtcacccg ctgcctttcc
      661 tcctgcgtgg cggcgtgccc gagcaggtcg gcagtttcgg cacgttcctg ggcgtcggcg
      721 acgaggcgaa ctggggcgtc gtcgagctca cgctcgagcc gggcgaccag ctcttgctct
      781 acaccgacgg cgtgatcgac gcgcgcggtc tggaggaacg gtttggggag gagcgcctga
      841 tggagactct caccggcgcg cgggatccgg cgggcgccgt cgaggctgtg gtggccgcgc
      901 tcgccgcgtt ccaggcaggc tcacaggccg acgacaccgc gctcgttgga atccggctag
      961 cgcctccgga aggcgtgcgt cacccacagg aaaaccctgt agaaacgctg tcagacgcct
     1021 gattggtgcg ggtcccggta ccgcatacct ggtagcgtgc cccacgcttc cacattggta
     1081 cgcagaggct cgcaggcggt tgtggagcag cacagggaag agctcgcagg aggacccggc
     1141 gcggctcgtc tggggcgccg gatcgtggac gacgtcgtcg gcgccgaggt ggacgacgag
     1201 acgcgctacg acctgaaggt gctcgtctcg gaactcgtta cgaacgcggt gcgacacggc
     1261 gccgcaggcg tggaggacac gatcctccta acactcggcg ttacatcgaa gcgcgtgcgg
     1321 gtggaggtct gggactccgg cccgggcttt gacccgccga ggcagccgcg cgcgcgcatc
     1381 gcaagcggcg gtgggaacgg actcgtcatc ctcgaccggc ttgcgagccg ctggggagtt
     1441 gccgctggcc gtcggcgcaa atgcgtttgg ttcgagatgg agatcgcctg agtctgcggc
     1501 acgtcccctc gcgggcgcga cgctgagcag gagcccgccg acgatcgcgg ccgcgccgag
     1561 cagctgcgaa cggctgaacg ggtcgccgaa cgcaaccgcc cccacggccg cgcccacgac
     1621 ggtctcgagg ttgagaaacg cgccggcaac ctcggcggcc acgcgcgcct ggccgtacgc
     1681 gtagagcgcg aacggcagca gcgagccgac cagcacgagc gctgcaaagg cggtgagcac
     1741 gtcgccgctc ggaagcgtgc gcggaacgcc ctcgaaggcc agcgcgaacg gcagcgtgac
     1801 tatcacgcct accgccatct gcaccgccgt gaccgcgacg gggtcgcgcc cgcggaggac
     1861 cgccggctgc gcggccacga aaagcgccga gagcgcggcc gacgcaagca tgagggcgtc
     1921 gccaccggtc gaggcggttc cgccggagcc tgcaacgaga cccgcgcctg cgatcgccgc
     1981 cgcgaagccg gtccactcga cgcgtccgct gcgcccctca ccgcgggcga gcgtggcgag
     2041 cgccaccagc gccggcacgg ctccgatgat caaggccgcg tgggtcacgc ttgtgagcgc
     2101 gattcccgtg ttctggagcc cgacgacgcc gccgtagaag agcgcgcccc agccgatcac
     2161 acgccacgag agggccccgc gcaggccgcg gcgcccggcg agagccagca cgggtgcagc
     2221 aatcgcgaag cgagccacgc cgagtgacag cgggccgagc caggcgagcg cgaccttcga
     2281 gaacggaatg gtcaggcccc agccgatccc ggctgctgcg agtgcgatca gggcttgtcg
     2341 gcggtccctt gccatgtatc gaattgtcgg cagtgaccac agatgcgccc agctcgatgc
     2401 gaccgttcgg cagttacacg aaatagaatt tcgtcgtatg gccataacgc ttgacagaat
     2461 tgacgtgacg ctcctcaggg agctgcagac ggacgccggg cggccaaacg tggagctggc
     2521 caagcaggta gggctctccc ccgccgcgac gctcaaccgc gtccggcggc tgcgcgacgc
     2581 ggggatcctg gggccagcgc gagcacgagt cgacgccaac gcggccgggt ttccgctgct
     2641 cgtgtacgtg ctcgcgacgc ttgccgagca cgacgaggcg gcgcaccggc gcttcaaggc
     2701 cgccgtcgac aagatcccga acgtcgtctc ggccgactgg gtgaccggcg agaccgacgc
     2761 gctgctgcag gtggtggcgc gtgacgtgga cgagcttcag cgcgtgctgc tgctgctctc
     2821 ctcacgcgcg ggcgcgcagc ggctggtgac gctgctgcgg ctggaggagc tgaagcctgc
     2881 ggcgccgctg cccgtggcgc ccgcatcgct gagcgagcgt gccgctgcgc gcgaaggcgc
     2941 ctgattcggg cggccgagtt accccgtgaa gcgggtgggt atgcgccacc tccacgatcc
     3001 ttcaaggagg gaacaagatg gcggacgttg cgtcccccgc tgcgagggcc cgcacaggcc
     3061 ccgatgatca gggagcggga gagaaggcca aagagaccgc tcagcaggca gcgggccagg
     3121 cccaggagaa agctcaggag gtggccggcc aggcgcgcgg ccgcgtccgc gagcaggtag
     3181 accagcgaag cagccaggtc ggaaggcaac tgagccagtc ggcccaagac gcgcggagca
     3241 tcggcgagca gctacgccgc cagggcaagg acgggcccgc ccggctcgcc gatcaagcgg
     3301 cgaaccgcgc cgagcgcgtc ggcggctacc tcgagaactc ggacggcgac aagctgctcc
     3361 gggacgtcga ggacttcggg cgccggcagc cggtggcggt cgtgctcggc gggctcgtga
     3421 tcggattcgc ggcgtcgcgg ttcctgaagg cctcgagcga ggaccgctac cggtccacga
     3481 gcgtggaccg ctacccgtcc acgccccggc cgagcaccgg acgtgagagt ttcggcggca
     3541 ctgtgccccc gagcccggcc cttggcgaaa cgggacttcc acggaccagc taggggcgct
     3601 catgaccacc acagacgaaa accaggccga gctgcgggag cggccgatcg gggagctgct
     3661 caggcagctc tcgcaggaga ccacgcagct cgtgcgtcag gagatcgagc tggccaaagc
     3721 ggaggtctcc gagaagggca agaaagcagg ccagggcgcc ggcatgttcg gcggcgcagg
     3781 agtcgccggc ctgctcgccc tcggcgcgct aaccgcgtgc ctgatcgtgg ctctcgatct
     3841 cgccatgccc acatggctgg cggcgctgat cgtgaccctg atctggggcg ccgtcgccgg
     3901 cgtcctcgct ctcaccggac gcgagcgtgt tcgagaggcc gcgcctgccg tgccggaaca
     3961 gaccgtcgag actctcaagg aggacgtgca atgggccaag acccagacgc catccgcgcg
     4021 cagatagagg aaacgcgcgg ccagatgggc gaaaccgccc aggcgatcgg ttacaaggcc
     4081 gacgtgaagg agcggaccaa ggagtccgtg caggagaaga ccgaccgcct gaagtcgaag
     4141 atcaccggcg cgggctcgcg gttgaacgag gcgaccccgg aaggctccga ggtgaagcag
     4201 acggcccaga gggcggcggg catcgcgcag cagaacccga ttggcctcgc catcggcgcc
     4261 gtcgcggtcg gattcctcgc cggcctcgcg ctcccgagga cgcgcatgga gaacgaaagg
     4321 ctcggcccca tcgcagacga tgtgaaggag cgcgccaagg agacagggca ggaagccctc
     4381 gagcgcggta agcaggtggc gcaggacgca gcccagagcg cagcggaagc cgcaaaggag
     4441 agcggtcgcg agcagagcga gggccttcaa cagagcgcgc aggagagcgc acaggaggtc
     4501 cgcacgcggt cctgaacgac gcgggacagc tgcgcccgcc gcctagacgg cgggcgcagc
     4561 gcccgggtgc ttaggtggcg acgggtcgga gctcgaccgg tccgcaggcg gccagctcgg
     4621 ccaccagctg gttcgcggtc tcgtcgatcc agcggccgga gtccgtgaga cggacagcca
     4681 gctcgatggc gtcggtcagg gccgactcgg cggtgtcaat gtcctcggtg aagagcagct
     4741 ggtcagcggt ctcgcgaatg ctgtccagct cgctcggctg cagcttgctc ggcccgaggt
     4801 tctcgatcat cttcattacg cgcccgtagg cctgcgcacg ttcacaggtc atggggactc
     4861 cttttcgggg ggagcgcccg agactaggag gcaaccggga cggcgtcgag tggtatgcgc
     4921 gtgcgagcga taggtagcgc gaatcagcac tattggccgc gcgccaccgc gaaactgcag
     4981 caataaaggg ggagcgcggc gcggcgcgac agtctgatcc ggacctgggc tgtttgaaca
     5041 ctgatagtca tggtcaaaac ggcccaggtc cggccgaggc ctccggcagc cccacccgat
     5101 cctcgttttg ctgcttttcc ggtctcgacc cggcgccgct accgcccctt ccgctccccc
     5161 ctctcgcgtg tcggcagctc cgccgacacg ttcgacagct tcaccgagaa cggcaccttc
     5221 gacacttggt agtagttcgc gtcgcgcccg agcagctcga gcttcacggt cgagccgcgc
     5281 gcgaagcggt gattcgtccc gtgcagctgg aagcggatcc gcccgcgctg gttgcggtcg
     5341 agccgcacca cgccgcggtc caccagcagc tgctggccgc tcgtcacctc ccacaggcgg
     5401 gccgccaggt acgggaacac gccctgggcc cggatcgagg cgctgatcgt ggtcagcccg
     5461 agcagcgtga agccgggcga cttcgcctcg accaccgcgg tccccggcgc cttctcggcc
     5521 gcgacggtct tgcacgggtt gtcgaagcct gcgatcgggt cgaacgcctt gccggtggcc
     5581 gggttgccgc cgtcggacgt ggcggtctgc gccttgcgac cggacatcgt gaacgttcca
     5641 gggtgcgccg cgtcccacga cttcgagcgc actagcccgc tgtccggcgc cgtctgcggg
     5701 caggtcaccg agccggtgta gacgctcccc ggcgcgggcg ctctgccctt gccgcgcaga
     5761 cgcgcgtcga agaacgacga gatcagcttc gccccctcct cggtcgtcac tgtcttgtta
     5821 ccgcccggcg gatggccgat gttcaagaac gagagcgaca ccggcgcgtt ggcgtgccgc
     5881 gaccgaatca ggttgtacgc gcgcagcgcc tggccgacgg ggaacaggtc gtcgcgccac
     5941 ccgttcgaga tcaggagcgg cgcgggattg ccggggatcg agatcgccga gtggaagcgg
     6001 tggagctccg agacgatctt cggcacgcgc ttgtccgcga agtcgcccgc atcggtaacg
     6061 gccttccagt tggcgaggtc cgcgcgcggg tcggcgcccg gcggcgcgac gaagcctgac
     6121 gcggcgccgc cgatgtagag cccgtcgatg tacgagatga tcggcacgcc caccggggag
     6181 gagtcgatcc ggctcgtcgg cacctcggtg tcgagccagc ggccgttcgg gacgagcgcg
     6241 gaggcgaggt ccgaccaggg aatgatcgga gccgccgccg cgatctgcag cggcgtgccc
     6301 ttcgggctct tccacgccgt gaacttgccg ttcgttaggc gcacgcggtt cttgaggaag
     6361 ccgagctcca acgcctgccc gccgccgtac gagccgccgc tcacgccgat gcccttcgga
     6421 tcgacgatgc cctcgtccac cagacggccg agcagcagct gcgtgtcgcg cgcctcgtag
     6481 cgctggtcgc cgaggtggat ccagcccttc gcgcagtccg gcgtgcgact tgccacgctg
     6541 ccgcacgagc gcccgaagcc gcgcgccgac agcgtgatca ccgcatagcc gcgctgggcg
     6601 tagtacaggt tgttcgcgaa gcgcttcttg gggtcgttcg cgtccgccgc ctcgtatgcg
     6661 gccttgctgc cgccgtagcc gtgcatcagt accagcgccg ggtatggccc gttgccgctg
     6721 ggcggaagag tgacatccac gtcgatcggc acgccgtcga acgtgggcac gcgctggccc
     6781 aggtcggtgg tcgggcagaa gcgcgcaccg ccctgcgaga tgcaggcgtg cccgaacgga
     6841 gccgggccgg cggcgaaagc actcgatgtg agcgcggcgg aaaggaaaag cgtggcggcg
     6901 aacgccgaca cgcagcgggg gaggtgcatg gccaaggatg ttcctaccgg tggctgcgag
     6961 cggagcgaac cctataggga cgccacgctg gtagcttcac ccgcctcgat ggcggctcgt
     7021 ccaggctacg cccacgtgct cgcgatcccc acgcgctggg cggacaacga cgtctacggc
     7081 cacgtcaaca acgtcgagta ctacgcgttc ttcgacaccg tgatcaacgc gtggctgatc
     7141 cgcgagggag ggctcgacat ccaccgcggc gaggtgatcg gcgtctgcgc cgaatcgcgc
     7201 tgcgagtaca aggcgccgct cgagttcccc gagaccgtcg aggcggcact gcgtgtaggc
     7261 cagctcggcc gctcgagcgt gcgctacgag atcgcgctgt tccgcgaagg ggccgacgag
     7321 cccgcggcca ccggccactt cgtgcacgtg ttcgtggacc gcgaggcacg gcggccggtc
     7381 gagatcccgg agcgcctgcg tggtgcgctc gagcggctgg tggtggacgc atgaaggcgc
     7441 gcggcgccgt gctgcggcag atgggactgc cgtcgccgta ctctgagtcg cggccgctcg
     7501 agctcgcgga ggtggagctc gacccgccgg gtgagggcga gctgctcgtc cgcgtgggcg
     7561 ccgcgggcct gtgccactcg gacctctccg tgatcgacgg ctcgcgtccc cgggtgatgc
     7621 cgatgctgct cggtcacgag gccgcgggcg aggtggtcga aaccggcccg ggctccaccg
     7681 gcttcgcggt cggcgaccac gtggtgctcg cgttcgtgcc gagctgcggc gaatgcgggc
     7741 cgtgccgggc ggggcgagct gccctgtgcg agccgggtgc ggctgcgaac acagcgggca
     7801 cgctgctgtc cggcgcgcac cggatccacg gcgagaacgg cgaggtgctc aaccaccacc
     7861 tcggcgtgtc gtgcttcgcc gaccacatcg tcgtgtcgca gcactccgcc gtgcgggtgg
     7921 atcccgagct tcccgtcgag gtcgcggcgc tgttcgggtg cgccgtgctc acgggcgtcg
     7981 gcgccgccgt caacagcgcg caggtcgggg tcggcgatcg cgtcgccgtg ttcggcctcg
     8041 gcggcgtcgg cctcgccgcc ctgctcggcg cacgggtcgc cggcgcggca gagctgatcg
     8101 caatcgacgt ggtacccgag aagctcgccc tcgcggccga gctcggcgcc acgcatacgt
     8161 tggcggccgg cgacgagacg gtcggccaga ttcgggagct caccggcggt ggcgccgaga
     8221 aggtgatcga gaccgtcggc aacgagcgcg tgctcggcca ggcctacgcg gccacacgcc
     8281 gcggcggcac gacggtgtcg gtcggcctcc cccaccccga ccgcatgctc tcgatccccg
     8341 cagtgagcct cgtcgccgag gagcgcacgc tgaaaggctc ctacctcggc tcgtgcgtgc
     8401 ccgagcgcga cctgccgcgc ttcatcgaga tgtatcgcga gggacggctt ccggtggacc
     8461 gcctgctcac gcatcgcatc tcgctcgacg aagtgaacga gggcttcgac aggctcgcgc
     8521 gcggcgaggc cgtgcggcaa gcgatcgtgt tctaacgcgg cggcgggcgg acccgcgcaa
     8581 gccgacggta gagccagccc tcggggagcg acacgagagc gaacagccac agcagcggcg
     8641 cgaagatgaa cggcagcgcg atcgatacga gcagctggct cggccagcgc acgagcacca
     8701 tgcggacttt gtccgcgacg tacagcacga gcaggagcag cggcagcagc gtccaccaca
     8761 gcttcggtcg cgccgacagc gagctcgtca cgtccgccca gttggccacg agctgccaga
     8821 tccccacacc caccagcgcc gcgatcgccc cgcaaaggaa ccaggacagg aagagcggct
     8881 tgaggaatcg cttgctcccc gcgcgcagcg cccgcgtgta ccagccgggt ctcgcggcct
     8941 gtgggccgac gaggtcggag accccgctga actgccagcg cgccgggtcc tcgccgggcg
     9001 gcgcgtcggg cacgaactgc gcgaactcgc cgtcgccagg gaagaacgcg ccggtcatgc
     9061 ggtagccgtc gaggctcagg gtgaacgcct cgctgtcgcc gaacaggtcg aggtccgtgc
     9121 gcacgcgcgc gagcagcgac tgcacctgct tcgccactcc gaacgcttca gcgccgacgt
     9181 gcggggcctg ctccgagggc gcatacgcgt actcgtcccc ctcggcgagc ggcggctcga
     9241 catgccttcc gagcccagcc cgcaggtgca tcagccgcag gccgtgaccc gcggccgcgc
     9301 cctcccggct cgcgcgcacg agctgctcgt tgcgcagtcg cgcgccgtag atgctggtcg
     9361 agcgcccgag cacgctgagc acgcgcgcgc cgggctgcgt ctgctcctgc atctggccgg
     9421 atgcgtcgct cacgatcagg tgcgtgcagc cgcgatcgaa cagcccctgc acaccctggt
     9481 tgtcgtgcac accgccgtcg acgagctgca cccgccggcc gtcgcggtac atgccgctga
     9541 tcgagagcgg gtggaagaga ccgggcacgc aggccgacgc ggccaccgca agcccaagcg
     9601 ggaaatctcg ctgcttctcg gcgatcggct tctgaccggc cggcttgccc agctcgccgt
     9661 agtagccctg gccaagcagc tcgttcttgt cgacgtcgga catcacctcg cgcatcgcgt
     9721 catcggcggg catcggctct cccatccgca cggcctcgaa gcgccagttg tggccggtgt
     9781 tcagcgaggt cgcattgagc aacaggatcg ggaccttcgc ctcgcggccc gcgttgtgca
     9841 cgtcgggatc gaacggctcg ctcacgccgg ccggctggat gagcagctcg cgcatctcga
     9901 tctgcttgtc gatcaagcgg tagcgcttcg gacgctcgcc aggccaggcg ggcttgtaga
     9961 agtagcggtc gtagagatcg ccgatgcggt cgctgcgcga gtaactcagg cgcgccatgt
    10021 agaagttctt cgcgaggttg aggaacacgc gcccgcggat gttccggcgc gtggcctcga
    10081 gaaactcgtc ctcgagcgcc tgcacctcct ccacgtagcg ctgcggcgag gggtccgcgt
    10141 cctgctcgag cagcttcttc gcgtgcaggt accagagcgc gccgacgatc gagccgcccg
    10201 acacggtcga aatcacctcc acccgccgca gcacaccgag ctccgccagc cgcgcgagca
    10261 cgcctatgtg gaagaacgac gcacggaacc cgcctcctga gagagccagt cccagcttcg
    10321 agccgtcggc ctccacgagt ccaacgtctc accgccgcac gaggtggtca agtcggcgcc
    10381 gttcgtggat gatgcgcgcc gtggcaaccc accgccaagc cgccgtgaac gggatcgagc
    10441 tgcacgtggc cgaggagggc tcgggtccac cggtgatcct cgcgcacggc ttcccagagc
    10501 tctggtactc gtggcgcgac cagctgccgg cgctcgccga cgcgggctac cgcgtgctgg
    10561 cgccggacat gcgcggcttc ggtggcagct cggcgcccgc cgacatcgag gcgtacgaca
    10621 tgttcacgat cgccgacgat ctgatcggcc tgctcgacga catcggcgaa gagaaggcgg
    10681 tgctcgtcgg ccacgactgg ggcgcgaacg cggtgtggtg gacggcgctc gcgcacccgg
    10741 agcgagtcgc ggcggtggtc ggaatgagcg tgccgatcgt gccgcgcgcg cccgccccgc
    10801 cgatcgggct tctgcgccag ggcctcagcg acgacttcta catgatctgg ttccaggagc
    10861 cgggcgtcgc cgacgaggcg ctcagccgcg acgtacgccg caccttgacc acctcgcgcg
    10921 tgtgggacgc gaactgggcc gaaagggatg acgaccccag gcggcccgac tggctgagcg
    10981 aggaggagct cgccgtctac gtcgaaacgt tcacgcggac aggcttcacc ggcggcctca
    11041 actggtaccg caacatcgac cgcaactggg agctgagcga gccgatcgcc gaccggcgcg
    11101 tggaccagcc gtcgctcttc atcgccggga cacgcgaccc cgtgctcgcc ttcatgcctc
    11161 ccgaggcgat ggacgggatg gtcaccgact tacgcggcaa gctgatgatc gagggcgccg
    11221 gccactgggt gcagcaggag cggcccgacg aggtgaattc agcgctgctc cgcttcctgc
    11281 gcgaggtcgg ctggtaggcg cgcagccgcg aggcgtacga tctggcggtc atgaccacgc
    11341 accaaaccga gctcgacatc gcgatgcggg tgctgaagct cgtgctcgtg atggtggctg
    11401 tgctggtcgg catcgcgctg ctgaccgttg cggccaacgc gctgttcacc tgaccccaca
    11461 cctctcggcg gtgtgacaaa ccgcaccagt cacttgggtg gtggctcgct agggatgccg
    11521 tcgcccgcgg cgtaagttgc catcagcagc tcacgccccc cacatcggag agccaccatg
    11581 accggacagc acgtctacat ggcagcacag gcaaggcacg cacaagcaca tctcgcacac
    11641 cgccactccg ctccgtcgcg caaatcccgc agcgaggccc ggtccaccaa actcgaacgg
    11701 atccggttct acctgcagcg tcgcgcctac gcgactagct gaactctcga acagccaccg
    11761 cgaagcccgc tcgatgtagc gggcttcgtc gatttcggtt cagcgaggcc ccgctcgatc
    11821 cagataccag tggcgtccga cggacgcgat ccatctgtcg tcgccgaccg gatttagcac
    11881 gaatcgactg attgttcgct ggtcgcctat ctgatctgtg tagagcaggt cgatcgagat
    11941 cggctcgcga tcgtcgagcg ctctagcgat ctcggggtac cccgcgtcgt gctggtcgcg
    12001 gaacgccccc tgccagagcc cgatgtcgcc tgccgggatg tataggtctc gcgtctgcgg
    12061 tcgcatctcc tcgagctcgg cgtgcgctat gtcgggcctc gcgagtccgg cgcgagcgtg
    12121 ccaaccctgc aggacagcga tccccaagcc gacgttccgc aggctgagca cgagatagag
    12181 cacgccgtct gagtgctcgg cggtcccacc gccgcccgag acccgggccc agtggcggtc
    12241 gacgaacatg atcttctgct cggcgtcgtc gagccgcgac tggacgagca ccggccggcg
    12301 ttgctcctgg agcgcgatct ccgcggtgcg ggccgagcgg ttcgccgagc gcacggaccc
    12361 gtacgtggcc gccgccagcg cgagcgtgcc gccggcggtc gcgagcgctg agatcgtcgc
    12421 ccagtcggcc atgccggcag atgtaaccgc cttttaggcg atcgcgacga cgcccggctg
    12481 gggctcgggc tcaggctcgg gctccggagc cgagtccagc cggggccgtt gggcgcggga
    12541 cagcgcacgc agcaggccgt gagtcggcag gtgggtggag tccgggcgtg tgatcgctgc
    12601 ggtctggaac gagagctcgg tctcgtcgag cggcaggaag cggacacccg gggcggcgta
    12661 gcggtcggac acggactccg gcaggagcgc cattccggcg ccggacgcca ccgcgagaag
    12721 cgcctcctcg acatgcgtgc cggctagctc cacgagcgtc ggggacagcc cgctcgcgtg
    12781 gcacgtagcc acgaccgcgt cgtagaacgg acgattctca tcgcgtggga gaagcaggat
    12841 ccgctccggc gcgagccggc gcaggctgag cgcgcccttc accgcgtcac ggtggccgat
    12901 cggcagggcg gcgaccgccc gctgatcgcc tagctgcgtg atcttgagcc ggtccgccac
    12961 ggccggcatc ggcacgatcg cggcgtcgag ccgcccgttg cgcacggcct caatgaggtc
    13021 ctgcgacagc cccgtttcga gggcggtctc gagctgaggc gtcgcagaag agagattctg
    13081 gagcgcgcgc ggcaccatcg cgggcagcgc cgacgcgacg tagccgatgc gtaggcgggc
    13141 tgattcacgg tcccgcgcgt tgcgggcggc cagccgcgca acttcggcct ggcgaaggac
    13201 gcttcgggcc tcctcgagca tggcggcacc tgagctcgtg agcgacacat tccgctgcgt
    13261 gcgttcgagc agctggacgc ccagctcttg ctcgagcttc cgcacctgct cgctcaccgc
    13321 gggctgcgcc acgtgcagtc gctcggccgc acgccggaag tggagctcct cagcgactgc
    13381 tacgaagtac ctcaagtgcc gaagctccat gtcattccac ctcagttgat ctcaagacag
    13441 tcagttcttg gcgatccccg gttcgagtgc gaactgtcgc gcgaatgcaa ggcccgggac
    13501 atacccccct ccccgagaac cgccttagcc cgaccggagt gccagggtcg ggctagccct
    13561 accgcggggt gcgatgaacc cgatcaagtg atcgcccgac gcgattgccg aggcacggtc
    13621 gtccacgtat cgtcggcgac gaagatttcc cccgactcgc tgagagggaa cggagcgcgc
    13681 aatgaaagcc cgccactcca tcctgtcgct catcgtcgtc gccgctgtca ccatccttgc
    13741 cgccggctgc ggcgggggat cgagcaacgc gaactcctcg aactcgaaca caagcaccgg
    13801 cgcttcctcg cagcctgcgc cgagctcggg gtcgaacgcc gtgaagatca gcaacttcac
    13861 gttcgtcccg gcgacactca gcgtgaaggg tggtgcgagc gtgacggtga ccaacaacga
    13921 cagcaccacc cacacgttca cgagcgacga cggccacagc ttcgacaccg ggaacatcgg
    13981 tacgggctcg tccaagacgg tctcggtgag caaggccggc cgctacccct accactgctc
    14041 gatccacccg ttcatgcacg ggacgcttgt gatcaagtag gacggcggcg cctgccatgc
    14101 tggcggggtg agcacgaacg gatccgggac cgccctgctg gtcatcgaca tgcagcagga
    14161 agtggtggat tcctgtccgg acggcacggg gctgatcaac cggatcaact cgctgtcgcg
    14221 gcgcgccgct cacgtcggcg tgcccgtgat cttcatccag cacgagggcg aagacgaact
    14281 gctcaaggga acgcccggct gggagttcgc cgccggcctc gagcgagcga gcgattcgct
    14341 actcgtcccg aagacctacc gcgacgcctt cgcgaacacc gagctcggtg acctactcga
    14401 gcgcctcggc gtgcgccggg tcgtggtgac gggcgctcac tccgacttct gcgtgcagac
    14461 gacggcgctt tccgcgctct tccacggctt cgacctcgtg ctcgtgagcg acgggcatgc
    14521 ggcggagccg tcgccgcacg aggacgggtt aatgccggag gcgctcgagg cgttcgtgaa
    14581 cgcgcgcttc cgcacactgc gctatccgga gcgcaccgtc gaggtcgtgc cggcggctga
    14641 cgttgagctc taggggcata cagccagagc tgtgggtcga ccgcgccgcc gacgccgtcg
    14701 ctttctacga ggcggcgttc ggtgcgcggg tggtgcaccg ggtcggcgag ggtcaggaca
    14761 tcgtcgcgca gctcgtcgtg ggcgacgacg ccttctgggt gtccgccgcg tcgtccgaga
    14821 tgggacgctt cagcccccac gcgattgctg gcgcgacgag ccggacgttg ctggtcgtcg
    14881 acgatccggc atcggtggtc gaacgcgcgg tggccgcggg cgcgaccgag atcgacatcg
    14941 tcatcacgcg ccagcacgtc ctgac
//
LOCUS       DASVXS010000082         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_647130, whole genome shotgun sequence.
ACCESSION   DASVXS010000082 DASVXS010000000
VERSION     DASVXS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647130"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VF752_04910"
     rRNA            <1..308
                     /locus_tag="VF752_04910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            385..501
                     /gene="rrf"
                     /locus_tag="VF752_04915"
     rRNA            385..501
                     /gene="rrf"
                     /locus_tag="VF752_04915"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            610..993
                     /locus_tag="VF752_04920"
     CDS             610..993
                     /locus_tag="VF752_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854921.1"
                     /translation="MSVVAIIAIVLGALLVLFFVGGLVAALRRRARHEQTFEKNVAEA
                     DRALENARAVDKGWDRSELERVAREALAAHRSGVDYPELALVLVDDPPGVIEDRAHFV
                     ARGAGKDARVVLSRSELGWKLDRVE"
     gene            990..1577
                     /locus_tag="VF752_04925"
     CDS             990..1577
                     /locus_tag="VF752_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854922.1"
                     /translation="MRRLGTRERAPASISELIPGFTPENELESRVVQSPELLEGLAWG
                     RPRPGHPEGPVAAHVADLLERLERMNEPPERRSMLRFIILVHDAFKNKVQEWRPRTGE
                     NHHATRARRFAERFTDDERLLTTIECHDRPYGIWRKWKRTGKLDEERLEQFIDRLPDP
                     DLFMDFVELDGSTEGKKPEPIAWFRDELRKRRPAG"
     gene            complement(1574..2683)
                     /locus_tag="VF752_04930"
     CDS             complement(1574..2683)
                     /locus_tag="VF752_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854923.1"
                     /translation="MRFLACLAGILAALAVVAVHPADAKTAPDGVPCGKPQPLPQIGS
                     GPTRSGGHFELYAGYGHRTDVCGRHCVADGLFDAKGEFVTGDNACGVTLRGRKLNVAV
                     EPDCERLDFLYWGPISPEVARVVITLRDGRVLEARIFAGPTGWAMNFYGIALRDRSPP
                     ARIEARDAAGRIVALLEYPFGDLGLCSLRLRPLSAVHPLAAARGPTGRLIRATGYRAQ
                     AHLGRRAARVLCVWVSGESTHRDLETVPSFCTPRRPSSRYLSGVWGSACSDHDAMSAG
                     LTSTRVRRVRLEYGDGATVTVDTRRIPAALGTNSRFWLAVAHSQEVPVRVAAYAARGR
                     LLASRKSDFPFRLNCRRGNRSDEAAGLVIARATLR"
     gene            complement(2778..3815)
                     /locus_tag="VF752_04935"
     CDS             complement(2778..3815)
                     /locus_tag="VF752_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854924.1"
                     /translation="MGGTSATAVAAELPVIGTGPMATGGTFELRAGMGRQPGNPDRDL
                     SRCAEVRVRGEEDDRDCHLELGTRETAQFRGSSGYCLRHEALYWALIAKRVGSIDAVL
                     ENRTVVRAQRFAGPEGWPFDFAVLPVHTLKPIERVDVRDMAGKLIERRYLPFGDEGAC
                     GGQPPPLTPRFRLARVRMPSGAVLRISAFRRLAREGRHRMSMLCIAHSFRLPDGDLGS
                     SSSSGEDCVRHGPTRDPVDLSFSTDCDTGDAIAQGLAKRRVGAVRVEWQDGSSAWARL
                     VTLPAQLHAHSRYWLAVAHSKRMPVALDVYDRAGRRILHDRDRYPSKLSCRRGGVLFG
                     GESRVEHASQP"
BASE COUNT          674 a         1416 c         1382 g          621 t
ORIGIN      
        1 tcgtgagaca gttcggtccc tatctgccgt gggcgttgga gaattgagag gattcgtccc
       61 tagtacgaga ggaccgggat gaacggacct ctggtgtacc tgttgtcctg ccaagggcac
      121 cgcaggttag ctacgtccgg acgggataac cgctgaaggc atctaagcgg gaagcccacc
      181 tcgagatgag ttctcccatc cccttgaggg agtaagagcc gtggtagacc accacgttga
      241 taggccgcag gtgtaagcgg ggcgacccgc ttagccgagc ggtactaatg cttcgagggc
      301 ttgacggata ctttttgatg cccgctttgt gcggccagct gtgcggtttt caagctccgg
      361 agggggcgca ggtctcgcag aatctccggt ggctatagcg agagggaaac acctcttccc
      421 attccgaaca gagcagttaa gcctctccgc gccgatggta cttggcccgc aagggcctgg
      481 gagagtaggt cgccgccggt tcttgactca cgagccgcct tcggggcggc tcgccgcgtt
      541 tgtggcgata tgcagggctt ttgttgcagg tggcgcaggg tctgagctgc cacatacact
      601 ccggcctcaa tgtcggtcgt agccatcatc gcgatcgtcc tcggagcgct cctcgtgctc
      661 ttcttcgtcg gtgggctggt ggcggcactt cgccggcgtg ctcggcacga gcagacattt
      721 gagaagaacg tggccgaggc cgatcgtgcg ctcgaaaatg cgcgcgccgt ggacaagggc
      781 tgggatcgtt ccgagctcga gcgggtcgcg cgcgaggcac tcgccgcgca ccgctccggc
      841 gtcgattacc ccgagctcgc actcgtgctg gtcgacgacc cgcccggcgt gatcgaggat
      901 cgcgcacatt tcgttgcgcg cggagcgggt aaggacgccc gtgtggtgct gtcgcgcagc
      961 gagttaggtt ggaagctgga ccgggtcgag tgaggcggct cggaacccgc gagcgtgccc
     1021 ccgcctcgat ctccgagctc atccccggct tcacgcccga gaacgagctc gagagccggg
     1081 tggtgcagtc gccggagctc ctcgaaggcc tcgcctgggg tagaccgcgc ccaggacatc
     1141 ccgagggccc cgtcgccgca cacgtagccg acctcctcga gcgtctcgaa cgtatgaacg
     1201 agccgccaga gcggaggtcg atgctccgtt tcatcatcct cgtgcacgac gccttcaaga
     1261 acaaagtgca ggaatggcgc ccgcgcaccg gcgagaacca ccacgcgacg cgcgcccgcc
     1321 gctttgccga gcgcttcacc gacgacgaac ggctgctcac gacgatcgag tgtcacgaca
     1381 ggccctacgg catctggcgc aaatggaaac gcacgggcaa gctcgatgag gaacggctcg
     1441 agcagttcat cgaccgtctg cccgaccccg acctgttcat ggacttcgtc gagctcgacg
     1501 gctcgaccga aggcaagaag cccgagccga tcgcctggtt ccgcgacgaa ctgcggaaac
     1561 ggcgccccgc cggctagcgc agggtcgccc gggctatcac cagaccagcc gcctcgtcac
     1621 tgcggttccc gcgccggcag ttgagcctga acgggaagtc ggacttgcgg ctcgccagca
     1681 gccgaccacg cgcggcgtag gcggcaaccc tcacgggaac ctcctgagaa tgcgcgacgg
     1741 cgagccagaa gcgagagttc gtccccagcg cggccgggat cctgcgcgta tccaccgtca
     1801 cggtcgcccc gtcgccgtat tcgaggcgga cgcgcctcac gcgtgtggat gtgagcccgg
     1861 cgctcatcgc gtcgtgatcc gagcacgccg agccccaaac gccggacaga tagcgtgagg
     1921 acgggcgtcg cggcgtgcaa aagctcggaa ccgtctcgag gtcccggtgc gtgctttcac
     1981 cgctgaccca gacgcacagc acgcgtgcgg cgcgacggcc caggtgagcc tgcgcgcggt
     2041 acccagtcgc acggatcagc cggcccgtcg ggccacgggc ggccgcgagc gggtgcaccg
     2101 cgctgagagg cctcagccgc aggctgcaaa ggccgagatc tccgaacgga tactccaaga
     2161 gcgccacgat ccgtccggcc gcatccctcg cctcgatgcg cgccggcggt gagcggtccc
     2221 ggagcgcgat cccgtagaag ttcatcgccc agcccgtcgg gcccgcgaag atgcgcgcct
     2281 cgagtacgcg cccatcgcgc agggtgatca ccaccctcgc gacctcgggc gaaatggggc
     2341 cccaatacag gaagtcgagc cgctcgcagt cgggctcgac agcgacgttg agcttccggc
     2401 ctcggagggt cacgccgcag gcgttgtcgc cagtgacgaa ctcacccttc gcgtcgaaga
     2461 gcccgtcggc gacgcagtgc cggccgcaaa cgtccgtgcg gtgcccgtac cccgcgtaca
     2521 gctcgaagtg gccacccgag cgagtcggac ccgagccgat ctgagggagc ggctgtggct
     2581 tcccacacgg caccccgtcc ggagcggtct tcgcatccgc cgggtgcacc gctacgaccg
     2641 cgagcgcggc caggattccc gcgaggcagg ccaggaacct catgcgccga ccctacccgg
     2701 gcgggcggcg ggtcgctcag cgcggctcga cccagaggca ggctgcgacc accggccacc
     2761 ggcctggcct gctcgactca gggctgcgac gcgtgctcga ctcgggactc accgccgaac
     2821 aagacgcccc cgcggcgaca ggacagcttc gacggatagc ggtcgcggtc gtgcaggatc
     2881 cggcgtccgg cgcgatcgta gacgtccagc gctaccggca ttcgcttcga gtgggctacc
     2941 gccagccagt atctggagtg ggcatggagc tgtgctggga gtgtcacgag ccgcgcccag
     3001 gcagaacttc cgtcctgcca ctcaacccga accgcgccca cacgccgctt ggcgaggccc
     3061 tgcgcaatcg cgtcgcccgt gtcgcagtcc gttgaaaagc tgaggtccac ggggtctctc
     3121 gtcggaccgt gacgcacgca gtcctcaccg ctgctgctgc tgctgcccaa gtcgccgtcc
     3181 ggcaatcgaa atgagtgcgc gatgcacagc atgctcatgc ggtgcctgcc ttcacgcgcc
     3241 agcctccgga acgcgctgat gcgcagcacc gcgccggacg gcattcggac gcgggcgagc
     3301 ctgaaccgcg gcgtcagagg cggtggctgt ccgccgcacg cgccctcgtc gccgaacggg
     3361 agatatcgcc gctcgatcag cttgccggcc atgtcgcgga catcgacccg ctcgatcggc
     3421 ttgagcgtat gcacggggag caccgcgaaa tcgaacggcc atccctccgg gccggcgaat
     3481 cgctgggcgc ggacgacggt gcggttctcg agcacggcgt cgatggaacc cacacgcttc
     3541 gcgatcagcg cccagtacag cgcttcgtga cgaaggcagt accccgagct gccccggaac
     3601 tgcgcggtct cgcgcgtccc tagttcgagg tggcagtctc ggtcgtcctc ttcgccccgc
     3661 acgcgcacct ccgcacagcg agagaggtct cggtccgggt tgcctggctg gcgtcccatg
     3721 cccgctcgga gctcgaacgt gccaccggtc gccatcggac cggtgccgat caccggaagc
     3781 tcggctgcga ccgccgtggc cgaggtgcct cccaagacga ggcatgcggt aagaccacat
     3841 gaggtaagcc gtttcatgcg gcgcacccta atcgggcgtg cggccgctta gcgagcctca
     3901 gcccacaaac acggctgcgg caccggccgc ggctgaccga agcgcttgcg ccgcggccgt
     3961 gaccgggcac ttcggtggag cgcacttcgg cccgcgtgcg ggcacggccg tgcgaggcgc
     4021 tccgcctcgg cgcgctgcgc cgcggcgcgg gcctcgaggg ccctgcgcgc cgcggctgtc
     4081 gcgggcggta gag
//
LOCUS       DASVXS010000083         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1366277, whole genome shotgun sequence.
ACCESSION   DASVXS010000083 DASVXS010000000
VERSION     DASVXS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366277"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..658)
                     /locus_tag="VF752_04940"
     CDS             complement(143..658)
                     /locus_tag="VF752_04940"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8854925.1"
                     /translation="MDQNNPLEWRGCEMVDRDGDKIGTINEIYLDADTRQPEWAVVKT
                     GLFGRGHKFVPLKGADREGDYVRARYDKGQVKDAPDLDLLDGDLSVDEEQRLYEYYGI
                     DSVSEVGQPAGVGQQGGVSQEAPVGQEGEVAEQGTVAQGGGVRRVRLVRYVLEEVPVD
                     TGTTGTREGNQ"
     gene            complement(714..1289)
                     /locus_tag="VF752_04945"
     CDS             complement(714..1289)
                     /locus_tag="VF752_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8854926.1"
                     /translation="MPKPEARFQTTGGIVTVEEPDFRAFKRYLDNLNSRDPERVPDMF
                     TPKVIFEDSAWLTVMHGPDEIKEMLVSRWNAFPDLHFRVIEGPYATDDTSHMAVKMRL
                     TGTMSGGLAPPGFDPTDGKVELDVASFYDFADDDRVKRGRVIFDTAYLAQQIGAMPSP
                     NSLAMQADVVFQKVTAWWMRNRGPSLGGRLR"
     gene            complement(1336..2172)
                     /locus_tag="VF752_04950"
     CDS             complement(1336..2172)
                     /locus_tag="VF752_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8854927.1"
                     /translation="MAVDPRAYAAALPQAERRDVARLGRGETLLSATSARLRKVRTGG
                     TLTLIGGRRLRVAGVLPDPLAQSAEIVVDNRDARVETAARNVVALLKPDADISAAALA
                     RRAGDGARARLVLDRSEDRRGPASGGPARPAELKARFGEPSVALPYGQDWVRLDRGFV
                     RRNIVTRRVPILGSVTCHRDMVPALRAAMSELERRGLAGLVDPGDYAGCYAPRRIQPG
                     GALSLHAWGLAVDLNASANPFGGRSHQDRRLVRTVKRHGFTWGGEWPSIHDPMHFEFR
                     GR"
BASE COUNT          338 a          839 c          833 g          397 t
ORIGIN      
        1 tggtaatggc catgtgcggc gacgcgcttg cgctccatcg tcgcggtgcg ctggcgctgc
       61 atcgcgacca tcagcagcgc gagcaggatc aaagcgacga tcacgatcac gacgatcacg
      121 ccggtgctga ttgcgagaac ggtcattggt tgccctccct ggtgcctgtt gtgccggtgt
      181 ccaccggcac ctcttcgagt acgtacctga cgagccgcac gcggcgcacg ccgccccctt
      241 gcgcgacagt gccctgctcc gcgacctcgc cctcctggcc gacgggggcc tcttggctca
      301 cgccgccctg ctggccgacg ccggccggct gcccgacctc gctgacggaa tcgatcccgt
      361 agtactcgta cagacgctgc tcctcatcga cgctcaggtc gccatcgagg aggtctaggt
      421 cgggtgcgtc cttcacttgg cctttgtcat agcgagcgcg aacgtagtcg ccctcgcgat
      481 cggcgccctt cagcggcacg aacttgtggc ccctgccgaa caagccggtc ttcacgaccg
      541 cccattccgg ctgccgggtg tcggcgtcga ggtagatctc gttgatcgtc ccgatcttgt
      601 cgccgtccct gtcgaccatt tcgcaaccgc gccactcgag cgggttgttc tggtccatcg
      661 atgcctccct actatcgctc gtttgagcga tacccacctg gcttgtgagc taactaccgg
      721 aggcgcccgc cgaggcttgg gcccctgttg cgcatccacc atgcggtgac cttctgaaag
      781 accacgtcgg cctgcatggc gaggctattc gggctcggca tcgcaccgat ctgctgggcg
      841 aggtaggcgg tgtcgaagat aaccctgccg cgcttgacgc ggtcgtcgtc ggcgaagtcg
      901 tagaaggagg cgacgtcgag ctcgaccttg ccgtccgttg ggtcgaagcc aggcggggcc
      961 agaccgccgc tcatggtgcc ggtgaggcgc atcttcaccg ccatgtgcga ggtgtcgtct
     1021 gttgcgtacg ggccctctat tactcggaag tggaggtccg ggaaggcgtt ccagcggctg
     1081 acgagcatct ccttgatctc atccggcccg tgcatgacgg tgagccaggc ggagtcctcg
     1141 aagatcacct tgggcgtgaa catgtcgggc acgcgctcag gatcgcgcga gttgaggttg
     1201 tcgaggtagc gcttgaacgc gcggaagtcg ggctcctcga cggtgacgat cccgccggtc
     1261 gtttggaagc gtgcttctgg cttcggcatg cgattgaccc tctctcagcc gccgtgcggc
     1321 gtcaagccgt gcgcgtcaac gcccgcgaaa ctcgaagtgc atcgggtcgt gaatcgacgg
     1381 ccactcgccg ccccacgtga agccatggcg cttcacggtg cggacgaggc gacggtcctg
     1441 gtgcgaccga ccgccgaacg gattggcgct cgcgttcagg tcgacggcga gcccccaggc
     1501 gtgaagcgag agcgcgccac cgggttggat gcgccggggc gcgtagcagc cggcgtagtc
     1561 gccgggatcg actaggcctg cgagcccacg gcgctcgagc tcggacatgg cggcgcggag
     1621 cgcggggacc atgtcgcgat ggcaggtgac gctgccgagg atcggcacgc gccgcgtgac
     1681 gatgttccgg cggacgaagc cgcggtcgag gcgcacccag tcctggccgt acggcagcgc
     1741 gacgctgggc tcgccgaagc gggccttcag ctccgcgggc cgcgccggcc cgccgctcgc
     1801 ggggccgcga cggtcctcgg agcggtcgag cacgaggcgg gcgcgggctc cgtctccggc
     1861 cctgcgtgcg agcgcggccg cgctgatgtc ggcgtctggc ttcaggagcg cgaccacgtt
     1921 gcgggccgcg gtttccaccc gagcgtcgcg gttgtcgaca acgatctcgg ccgactgcgc
     1981 gagcgggtcc ggtagcacgc cggcgacgcg tagccgccgt ccgcctatga gcgtgagcgt
     2041 tccgccggtg cgtaccttgc gcagcctggc cgaggtggct gagagcaggg tctcgccgcg
     2101 ccccagccgc gccacgtccc gccgctccgc ctgcggaagc gcggccgcgt atgcgcgggg
     2161 gtcgacggcc accgcgtcga gcgggacggc aaagccgcgc ctcgtttgct cgaccctccg
     2221 gccgctactc gctcgcgccg acgtgagcag cgcggtgccg cgaccgatca cgctcgtgct
     2281 ccgcaccgcc ggacgcgtcg accaagcgcc aggcaagccg tccacgccag acaccggcaa
     2341 gcggccacgg tccccgcgtg tcgcgatcgt ctggggagcc gacgacccac gcgccgccgc
     2401 acgccgg
//
LOCUS       DASVXS010000084         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1366549, whole genome shotgun sequence.
ACCESSION   DASVXS010000084 DASVXS010000000
VERSION     DASVXS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366549"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF752_04955"
     CDS             <1..268
                     /locus_tag="VF752_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8854928.1"
                     /translation="AAARELRRLGRRVPRRARPDRGEGLAGLSPREREVAEEVASGKT
                     NRDIAATLFLSEKTVESHLARIYSKLGVHSRAALTGIVARQSTP"
     gene            330..572
                     /locus_tag="VF752_04960"
     CDS             330..572
                     /locus_tag="VF752_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854929.1"
                     /translation="MPTFILCHRHRPEECAIAVSAWKGFCSPLRHGHPLGSCLTGGHR
                     IWWTVEAGDSASALGQLPPYVAERTVAEQVREVPLP"
     gene            569..862
                     /locus_tag="VF752_04965"
     CDS             569..862
                     /locus_tag="VF752_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854930.1"
                     /translation="MTALHVCPACGSALVQLVAQDESESNRWWLTLRCPECETISAGE
                     FDQTAVEALERELERGAAELEANYLLLVQANMADALDRFLRALEADAIQPIDF"
     gene            922..1248
                     /locus_tag="VF752_04970"
     CDS             922..1248
                     /locus_tag="VF752_04970"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEX8854931.1"
                     /translation="MTQSENSPIIDRIESLVEEEQRLWHDESEGRLDDDGRQRLAIIR
                     AELDRCWDHLRRRRANPTAPDVIDPVPWPPNDLEGPEPEPPHLEHGVHGADEPAPDPD
                     VPRDIP"
     gene            1349..2257
                     /locus_tag="VF752_04975"
     CDS             1349..2257
                     /locus_tag="VF752_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8854932.1"
                     /translation="MSSSPDSPPTSEGYAQVGDVKLHYVEAGDGPLILLLHGFPEFWF
                     GWRLQIAPLAAAGFRVVAPDTRGYNLSSKPDGVAAYSADKLAADITGLIRELGAESAL
                     VVGHDWGGTIAWTMAMNNPEVVQRLAILNAAHPRRLQQGLRTPGQLRKSWYFFFFQPP
                     GLPERLVRARDWRFFRRFLGDARPAFTPEEMDRYIEAWSLPGAATGMLNYYRAAVRQS
                     PKQAEAQLRPISAPTLVIWGQRDRYLGPTLAEPDRNDVPNLDRVERLPDASHWVHHDE
                     SERVTQLLTDFFAPARTTQDRQTSTT"
     gene            complement(2287..2664)
                     /locus_tag="VF752_04980"
     CDS             complement(2287..2664)
                     /locus_tag="VF752_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8854933.1"
                     /translation="MSAITHLFAGVPVSDLDASIDWYTRFFGRPPDSRVGEEILWEID
                     EHAWLFIEPNAARAGAGRITLAVAELDELLERLTAQRIEHEPIETYSNGVRHVSVPDP
                     DGNAIAFAEPPEGANASPRSAAS"
     gene            complement(2676..>3567)
                     /locus_tag="VF752_04985"
     CDS             complement(2676..>3567)
                     /locus_tag="VF752_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8854934.1"
                     /translation="MRRAFAEAAVAQPDFLVVPAGRDVATAARGLGFPVVVKPLSLSA
                     SRGVIRADDEAAAASAGARVRAIAGEEAELLLERYVPGREVALEGLLRGGELEVLALF
                     DKREPMEGPYFEETILVTPSRLPVEVQARVAELAGRAAAALGLRDGPVHAELRVDGDE
                     VVMLELAARSIGGLCSRALRFGLGASLEEVILRHALGMPLDGLWREATASGVMMIPIP
                     GAGVLEEVGGQDRARAVAGVEGLEITIPRGREVVPLPEGDRYLGFIFARGEQPDGVEW
                     SLRKAHSKLDIQIADGARAA"
BASE COUNT          570 a         1299 c         1195 g          503 t
ORIGIN      
        1 cgccgccgcg cgcgagctcc gccgactcgg gcgacgcgta ccacggcgcg cgcgcccgga
       61 tcgcggcgaa gggttggctg ggctcagccc ccgcgaacgc gaggtcgccg aagaggtcgc
      121 ctccggcaag accaaccgcg acatcgccgc gacgctcttt ctcagcgaga agacggtcga
      181 gagtcacttg gcccggatct actcgaagct cggtgtgcac tcgcgggcgg cgctgacggg
      241 catcgtggcg cgacagtcga ccccataggg ggaccggttg caggattcgc ccggtaccgc
      301 gctcgcgcgg tagcgacgaa cctgggcaca tgcccacttt catcctgtgt cacaggcacc
      361 gccccgagga atgcgcgatc gcggtgtcgg cctggaaggg gttttgcagc cccttgcgcc
      421 acgggcaccc gctcggctcg tgcctgaccg gcggccacag gatctggtgg accgtcgagg
      481 ccggcgacag cgctagtgcg ctggggcagc tcccgcctta tgtggccgag cgaaccgtgg
      541 cggagcaggt gcgcgaggtg ccgctgccat gacggctctg catgtgtgcc cggcctgcgg
      601 ctcggccttg gttcaactgg tcgcgcagga cgagtcggag agcaaccgct ggtggctcac
      661 gctccgctgc cccgagtgcg agacgatcag cgccggcgaa ttcgaccaga ccgccgtcga
      721 ggcgctcgaa cgtgagctcg agcggggtgc agcggagctc gaggcaaact acctgctcct
      781 ggtccaggcg aacatggcgg atgctctgga tcgcttcctc cgcgcgctcg aagccgacgc
      841 gatccagccg atcgacttct gagttacaag cgacgctgcc gggtacatcg cccgctacgc
      901 gggacccgta ggatcaagcc catgacccag agcgagaaca gcccgatcat cgaccgcatc
      961 gagtccctcg ttgaggagga gcagcggctc tggcatgacg agtctgaggg gcggctcgac
     1021 gacgacggtc gccagcgcct cgcgatcatc cgagccgagc tcgaccgctg ctgggatcac
     1081 ttgaggcggc gccgcgcgaa cccgactgcg cccgacgtga tcgatcccgt gccctggccg
     1141 cccaacgacc tcgagggccc ggagccggag ccgccgcacc tcgagcacgg cgtacacggg
     1201 gcagacgagc ccgcgccgga cccggacgtc ccgcgcgaca tcccctagcc gcaccaggga
     1261 gcagaccagg gcattcacgg gcgcgatgtg gaggcgcgcg gaccaacctt cgtgcgtcac
     1321 cacgctcagt cttgagagga gatccctaat gagctcgtcg cccgactccc cacccacttc
     1381 ggaaggctat gcgcaggtgg gcgacgtgaa attgcattac gtcgaggccg gcgatgggcc
     1441 gctgatcctt ctgctgcacg gcttcccgga gttctggttc ggctggcggc tgcagatcgc
     1501 gccgctcgct gcggcgggct ttcgagtcgt cgcgcccgac acgcgcggct acaacctgtc
     1561 gtcgaagccc gatggggtcg cggcctacag cgccgacaag ctggccgccg acatcaccgg
     1621 tctcatacga gagctcggtg ccgagtccgc gctggtggtc ggtcacgact ggggcggaac
     1681 catcgcctgg accatggcga tgaacaaccc cgaggtcgtg cagcggctgg ccatcctcaa
     1741 cgcggcccac ccccggcggc tccagcaggg actgcgcacc ccgggccagc tccggaagtc
     1801 ctggtacttc ttcttctttc aaccaccggg gcttcccgag cgcctggtgc gcgccaggga
     1861 ctggcgcttc ttccggcgct tcctgggcga cgcgcgtccc gcctttacgc cggaggagat
     1921 ggatcgctac atcgaggcgt ggtcactgcc aggcgccgca accggaatgc tcaactacta
     1981 tcgcgcggcg gtgcgacagt cgccgaagca ggccgaagcg cagctgcgcc cgatctccgc
     2041 gcccacactc gtcatctggg ggcagcgcga tcgctacctc ggccccacac tcgccgagcc
     2101 cgaccgcaac gacgtgccga acctcgatcg cgtcgagcgc ctgcccgacg cgtcgcattg
     2161 ggtccatcac gacgagtctg agcgcgtcac acaactgctc accgacttct tcgcccccgc
     2221 ccgcacaacc caagaccgcc aaacgtcgac aacgtaaagg ccggatcggc agcgcagcgt
     2281 cactgcctac gacgcggccg atcgaggtga cgcgttcgcg ccctcgggcg gctcagcgaa
     2341 cgcgatggcg ttcccatccg gatcggggac gctcacgtgg cggacaccgt tcgagtaggt
     2401 ctcgatcggt tcgtgctcga tgcgctgggc ggtgaggcgc tcgaggagct cgtcgagctc
     2461 ggcgacggcg agggtgatcc ggcccgcgcc cgcccgcgcc gcgttcggct cgatgaagag
     2521 ccaggcgtgc tcgtcgatct cccacagaat ctcttccccg acgcgactgt ctgggggtcg
     2581 cccgaagaac cgcgtgtacc aatcgatgct ggcgtcgaga tcggacaccg ggacaccggc
     2641 gaaaaggtgg gtgatcgcgc tcatctcagg accgcctaag cggccctagc gccgtccgcg
     2701 atctggatgt cgagcttcga gtgcgccttg cgaagcgacc actccacccc atccggctgc
     2761 tcgccgcgcg cgaagatgaa gccgaggtag cggtcgccct cgggtagcgg caccacctcg
     2821 cgcccgcgcg ggatcgtgat ctcgagcccc tcgacgcccg cgacggcgcg ggcacgatcc
     2881 tggccgccca cctcctcgag cacgccggcg ccggggatcg ggatcatcat cacgccggac
     2941 gccgtagcct cgcgccagag gccgtcgagc ggcatgccga gcgcgtggcg gaggatcacc
     3001 tcctcgaggc tcgcgccaag tccgaagcga agcgcgcgtg agcataggcc tccgatcgag
     3061 cgcgccgcca gctcgagcat cacgacctcg tcgccgtcga cgcgtagctc ggcgtgcacc
     3121 gggccgtcgc gcaggccgag cgccgccgcc gcccgccccg ccagctcggc gactcgtgcc
     3181 tgcacttcga ccggaagccg cgatggcgtg acgaggatgg tctcctcgaa atagggcccc
     3241 tccatcggct cgcgcttgtc gaacagcgcc agcacctcga gctcgccgcc gcgcagcagt
     3301 ccctctagcg ccacctcgcg accgggcacg tagcgctcga gcaggagctc cgcctcttcc
     3361 cccgcgattg cgcgcacccg cgcacccgcg gacgccgcgg cggcctcgtc gtcggcgcgg
     3421 atcactccgc ggctcgccga cagcgacagc ggcttcacga caacggggaa gccgaggccg
     3481 cgcgcggcag tggcaacgtc acgcccggcc gggaccacga ggaagtcagg ctgcgccacc
     3541 gcggcctcgg cgaaagcgcg gcgcatc
//
LOCUS       DASVXS010000085         5711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_718507, whole genome shotgun sequence.
ACCESSION   DASVXS010000085 DASVXS010000000
VERSION     DASVXS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718507"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VF752_04990"
     CDS             <1..383
                     /locus_tag="VF752_04990"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX8854935.1"
                     /translation="ALGAADSLDAIVEVLVTQLVDYLEHDADGQVVLYEMFAASRNNE
                     ELQTEMGELYRRIRSGIARVLEDKERAGVVTLPAPADSVAAVLVALGDGMMMQVLSDP
                     DWDRDAAFALGIRMARLALGAADV"
     gene            complement(409..813)
                     /locus_tag="VF752_04995"
     CDS             complement(409..813)
                     /locus_tag="VF752_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8854936.1"
                     /translation="MSQENVEVVRQGLQAFADGGLDAMAEFWDPDIDWRAAEGAIDDV
                     GEMHGPVAVRRYFQDWLDTFDDAGVVVEELRDVGDDRVLSIQRMKGRAKLSGIETDLR
                     FAVVSTVRDGKVVRVREYLSVEDALEAVGLRE"
     gene            complement(876..2258)
                     /locus_tag="VF752_05000"
     CDS             complement(876..2258)
                     /locus_tag="VF752_05000"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX8854937.1"
                     /translation="MSEPVTMDKIVALCKRRGIIFPASEIYGGIANTYDYGHYGVLLK
                     RNVTDAWWNAMIRDRDDIVALDSAIIQHPRTWEASGHLAGFTDPLVDCKTCGQRFRAD
                     HLDLEHEEARCPRKPSVPAGQGPDCDLTEAREFNLMFETTIGPISETGQKVYLRPETA
                     QGIFLDFKTVLGFARKKPPFGIAQIGKSFRNEITPGNFIFRTLEFEQMEMEFFVPPGE
                     AERWYDYWLKERMAWYEGLGIRSDHLRLRAHEQDELSHYSSATSDIEYLYPIGFQELE
                     GIANRGDFDLTQHAKFSGEKLEYFDSGSGERYVPHVIEPAAGVGRTILALLCDAYDED
                     EIGGEQRTVLKLHPQVAPVKVAILPLVRKDGQPELAREVHGLLRNRFQTEYDEGGSIG
                     RRYRRQDEIGTPWCVTIDHQSLEDRTVTVRDRDTLSQDRVAIDELGDHLQARLDAPWT
                     SPKLTGAEAA"
     gene            2558..2857
                     /locus_tag="VF752_05005"
     CDS             2558..2857
                     /locus_tag="VF752_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX8854938.1"
                     /translation="MPIYEYRCDNGHTFEVMQRMTDEPLTVHEDCGAPVQRVFHPVAV
                     HFKGSGFYNTDYGKKSRSGASSNSESSSGSDNSSSSSSESASESKSSSSAAKSDD"
     gene            complement(2854..3918)
                     /locus_tag="VF752_05010"
     CDS             complement(2854..3918)
                     /locus_tag="VF752_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX8854939.1"
                     /translation="MPDAPVPEYKVYRSRRRLFDRLRSSDALRNFRRRERGEPGPEAP
                     PRERRPITPKRVLKWVALAVLFWLALSLVLFVISAQIEKGVSTNAKDALSGGGSLLTG
                     SNVLVLGSDARPPGSKEPGANPGGPSRSDSIMLLHVGFGTVRKLSILRDSQAQIPGHG
                     VAKINAAYAIGGPALAIKTVEGFLGNGLRVNHVLEVNFQNFPKLIDALGGIDVNLKRC
                     IRSNSFDGKTFRLRKGEHHLSGDEALRYARIRENFCAPREDDRARAARQQEVLSQMRS
                     KALSPGTFFRLPWVSWEAPRTVRTDLGGPGLIALFTDLTTGGGGKTDVLPFTQVNPDG
                     SLLVSQEDRRRAVEKLQGSG"
     gene            3943..4899
                     /locus_tag="VF752_05015"
     CDS             3943..4899
                     /locus_tag="VF752_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8854940.1"
                     /translation="MPTGRVIGIDAGGTKLLAGVVDERLLVHHRVHRVIRGLGQRKLL
                     EVMVEVVEEARAAEPDVGAVGFGIPSRVDADLGVSEGSVHLPLDGVPFRALMSERLGL
                     PVAVDNDANAALLAESRAGAARGARCAAMVTLGTGIGGGLLIDGELFRGNYGGGAELG
                     HMIVDIDGPECSCGSRGCLEALASGSAIGRLGEEAARKAPDSALGRALGAREELSGRL
                     VTELAHDGDEMARDVLASVGRSLGAGIANIVNIFEPQVVVVGGGAIAGGELLLEPARK
                     VVASRALYPARDRVRIAAAEFGDEAGMVGAALLAMDVVRTPY"
     gene            4911..>5711
                     /gene="rsmI"
                     /locus_tag="VF752_05020"
     CDS             4911..>5711
                     /gene="rsmI"
                     /locus_tag="VF752_05020"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX8854941.1"
                     /translation="MVCPTPIGNLEDVTLRVLAALREADVVACEDTRRTRVLLDRYGV
                     NARLVSYHEHNEERRAAELVEDMRAGKTVALVSDAGMPLVSDPGYVLVRAAAAAGIAV
                     EVLPGPSAVLAALVASALPADRWRFVGFLPRKKGELREVLSEPGTLVAFESPKRVPAA
                     LATLAELTPDREAAVARELTKAHEEVVRGTAGELAARYMDEPPRGEVVLVIGPLPGRT
                     ADEPGEEAVDALARLVEAGAKPRAAAAVVAELTGGSANALYRSLSSRRS"
BASE COUNT          830 a         1920 c         2039 g          922 t
ORIGIN      
        1 aagcgctcgg cgcggccgac tcgctcgacg cgatcgtgga ggtgctcgtc actcagctcg
       61 tggactacct cgagcacgac gcggacggcc aggtggtgct ctacgagatg ttcgcggcgt
      121 cccgcaacaa cgaggagctg cagaccgaga tgggcgagct gtaccggcgt atccgcagcg
      181 gcatcgcgcg cgtgctcgaa gacaaggaga gggcgggcgt cgtgactctg cccgcgccgg
      241 ctgactcggt tgcggccgtt ctggtcgcgc tcggggacgg gatgatgatg caggtgctat
      301 ccgaccccga ctgggatcgg gacgccgcct tcgcgctcgg cattcggatg gcccggctcg
      361 cgctcggcgc ggcggacgtc taggaacgtc tggcgaaact cgccacgcct actcccgcag
      421 ccctacggct tcgagggcgt cctcgacact caggtactcc cgcacccgca caacctttcc
      481 gtcgcggacg gtgctgacga cggcgaagcg cagatcggtc tcgatgccgc tgagcttcgc
      541 ccgccccttc atgcgctgaa tcgaaaggac gcggtcatcc ccgacgtccc gcagttcctc
      601 caccacgacg ccggcgtcgt cgaaggtatc gagccagtct tggaagtacc gacgcacggc
      661 gaccggcccg tgcatctcgc cgacgtcatc gattgcgccc tcggccgccc gccaatcgat
      721 gtcggggtcc cagaactcgg ccatcgcatc gaggccaccg tcagcaaacg cttggagccc
      781 ctgacgcacg acctccacat tctcctgcga catcgcccgc ccagtatccc gcaggcgacg
      841 cgcggtgcta cgccgcgagc ggcgcggccc gagagctagg ccgcttcggc gccggtcagc
      901 ttcggcgagg tccagggcgc gtcgaggcgc gcctgcaggt ggtcgccgag ctcgtcgatc
      961 gcgacgcggt cctgcgaaag cgtgtcgcgg tcgcgcacag tcaccgtgcg gtcctcgagc
     1021 gactggtggt cgatcgtgac gcaccacggc gtgccgatct cgtcctggcg ccggtagcgc
     1081 ctgccgatcg agccgccctc gtcgtactcc gtctggaagc gattccggag caggccgtgc
     1141 acctctcgcg ccagctccgg ctggccgtcc ttgcgcacca gcggcaggat cgcgaccttc
     1201 acgggcgcga cctgcggatg cagcttgagc acggtgcgtt gctcgccgcc gatctcgtcc
     1261 tcgtcgtaag cgtcgcagag cagcgcgagg atcgtccggc ccactccggc ggccggctcg
     1321 atcacgtgcg gcacatagcg ctcgccgctc ccggagtcga agtactcgag cttctcgccg
     1381 gagaacttgg cgtgctgggt gagatcgaag tcgccccggt tggcgatgcc ctcgagctcc
     1441 tggaagccga tcggatacag gtactcgatg tcgctcgtgg cgctcgagta gtggctcagc
     1501 tcatcctgct cgtgcgcgcg aagacgaagg tggtccgagc ggatgccgag gccctcgtac
     1561 caggccatgc gctccttcag ccagtagtcg taccagcgct cggcctcgcc cggcgggacg
     1621 aagaattcca tctccatctg ctcgaactcg agcgtgcgga agatgaagtt gcccggagtg
     1681 atctcgttgc ggaacgactt gccgatctgg gcgatgccga acggcggctt cttgcgcgcg
     1741 aagccgagca ccgtcttgaa gtcgaggaag atgccctggg cggtctccgg gcggagatag
     1801 accttctgcc cggtctccga aatcggaccg atcgtcgtct cgaacatgag gttgaactcg
     1861 cgcgcctcgg tgaggtcaca gtccggcccc tggcccgcgg gcacgctcgg cttgcggggg
     1921 cagcgcgcct cctcgtgctc caggtcgagg tggtccgccc ggaagcgctg tccgcaggtc
     1981 ttgcagtcca cgagcgggtc ggtgaagccg gcgaggtggc cggacgcctc ccacgtgcgc
     2041 gggtgctgga tgatcgcgct gtcgagggcg acgatgtcgt cgcggtcgcg gatcatcgcg
     2101 ttccaccagg cgtccgtgac gttgcgcttc agcagcacgc cgtagtgccc gtagtcgtac
     2161 gtgttggcga tgccgccgta gatctcggag gccgggaaga tgatcccgcg ccgcttgcag
     2221 agcgcgacga tcttgtccat cgtgacgggc tcggacatcg cgcacgaacc ctagcgagcg
     2281 accagcgacg agcgccaacg cgccaaggcg gctctcaaag accccgctct ttgcagagag
     2341 ggtgcagtta gtctcggaaa gggtgggata gcgtcggccg gcatggtgag ggtggctctg
     2401 cgaggggcga atcacatccg ggcctggatc gtctggcact cgttgaaggt gtccctgctc
     2461 gacgatctgg acacgccgcg gccgcccgcc gcggaatgaa ttagggccgt agctcgcact
     2521 cataggctgt agctggcact catatactcc gagtgccatg ccgatttacg agtaccgctg
     2581 cgacaacggg cacaccttcg aggtcatgca acgcatgacc gacgagccgc tgaccgtgca
     2641 cgaggactgc ggggcgcctg tgcagcgcgt gttccatccc gtcgccgtgc acttcaaggg
     2701 gtcgggcttc tacaacaccg actacggcaa gaagagccgc agcggtgcct cttcgaactc
     2761 tgaatcgtcg tcgggctcgg acaactcctc gtcgtcgtcg tccgaatcgg cgagcgagtc
     2821 gaagtcgtcg tcctcggctg cgaagtcgga cgactagccg ctgccctgca gcttctcgac
     2881 ggcccggcgc cggtcctcct ggctcaccag caggctcccg tcggggttca cctgcgtgaa
     2941 cggcagcacg tcggtcttgc ccccgccgcc cgtggtcagg tccgtgaaca gcgcgatcag
     3001 gccggggccg ccgagatcgg tgcggaccgt gcgcggcgcc tcccacgaca cccacggcag
     3061 gcggaagaac gtccccggtg agagcgcctt cgagcgcatc tgggaaagca cctcctgctg
     3121 ccgggcggcg cgggcgcggt cgtcctccct gggtgcgcag aagttctcgc gtatgcgcgc
     3181 gtagcggagc gcctcgtccc cgctcaggtg gtgctcgccc ttgcgcagcc ggaaggtctt
     3241 gccgtcgaac gagttcgagc ggatgcagcg cttcaggttc acatcgatgc cgccgagcgc
     3301 gtcgatcaac ttcgggaagt tctggaagtt gacctcgagc acgtgattca cgcgcagtcc
     3361 gttcccgaga aagccctcga ccgtcttgat cgcgagcgcc ggtccgccga tcgcgtaagc
     3421 ggcgttgatc ttcgccacgc catgtcctgg aatctgagcc tgcgagtcgc gcaggatcga
     3481 gagcttgcgg acggtgccga agccgacgtg gagcagcatg atgctgtcgg agcggctcgg
     3541 cccgccgggg ttggcgcccg gctccttgct tcccggcggg cgcgcgtcgg acccgagtac
     3601 gaggacgttg ctgccggtga gcaggctgcc gccgcccgag agtgcgtcct tcgcgttcgt
     3661 ggacacgccc ttctcgatct gggctgagat cacgaacagc acgagcgaga gcgccagcca
     3721 gaacagcacg gccagcgcga cccacttgag cacgcgcttg ggcgtgatag ggcgcctttc
     3781 gcgcggtggc gcctccggcc ccggctctcc gcgctcgcgc cggcggaaat ttcgcagcgc
     3841 gtcgctcgaa cgcagcctgt cgaacaggcg cctgcgcgag cgatagacct tgtactccgg
     3901 tacgggggcg tcgggcatgc gtgtgcgtat atcgtcgagc ccatgccgac cggccgtgtc
     3961 atcggaatcg acgccggtgg cacgaagctg ctcgccggtg tggtggatga gcggctgctc
     4021 gtccaccacc gggttcaccg ggtgatccgc gggctcggtc agcgaaagct gctcgaggtg
     4081 atggtagagg tcgtcgagga ggcccgggct gcggagcccg acgtaggggc ggtcgggttc
     4141 gggattccgt ctcgcgtcga tgccgatctc ggtgtctccg aaggatcggt gcacctgccg
     4201 ctcgacggcg tgccgttccg ggcgctcatg agtgagcgcc tcggtctgcc ggtggcggtc
     4261 gacaacgacg cgaacgccgc tctcctcgcg gagtcgcgcg ccggcgccgc gaggggcgcg
     4321 cgctgcgcag ccatggtcac gctggggacg ggcatcgggg gcggcttgct gatcgacggc
     4381 gagctgttcc gcggcaacta cggcgggggc gcggagctcg gccacatgat cgtcgacatc
     4441 gacggtcccg agtgctcgtg tggctcgcgc ggctgcctgg aggcgctcgc ctcgggcagc
     4501 gcaatcggcc ggctcggcga ggaagccgcg cgcaaagcgc cggattctgc gctcggacgc
     4561 gcgctcggcg cccgcgagga gctcagcggc cggctcgtca ccgaactcgc gcacgacggc
     4621 gacgagatgg cccgtgacgt gctggcctcc gtcggccgca gcctcggcgc gggcatcgcg
     4681 aacatcgtga acatcttcga gccgcaggtc gtcgtcgtcg gtggcggcgc gatagcgggc
     4741 ggcgagctcc tgctcgagcc ggcgcgcaag gtggtcgcct cgcgcgccct gtatcccgcg
     4801 cgcgatcgcg tccggatcgc ggctgcggag ttcggcgacg aggcggggat ggtcggcgcg
     4861 gccctcctcg cgatggatgt ggtcaggacg ccctattgac aggccgcctt gtggtctgcc
     4921 cgaccccgat cgggaacctc gaggacgtga cgctgcgggt actggccgcc cttcgcgagg
     4981 ccgacgtggt ggcgtgtgag gacacccgcc gcacgcgcgt actgctcgac cgctacgggg
     5041 tgaacgcgcg cctcgtctcc taccacgagc acaacgagga gcggcgcgcc gccgagctcg
     5101 tcgaggacat gcgggcgggc aagacggtgg cgctcgtgtc ggatgccggg atgccgctcg
     5161 tgtccgaccc cggttacgtg ctcgtgagag ccgcggcggc tgcgggcatc gcggtggagg
     5221 tgctgcccgg accgtcggcg gtgctggcgg cgctcgtggc ctcggcgctg cccgcggacc
     5281 gctggcgctt cgtcggcttc cttccgcgca agaagggcga gcttcgcgag gtgctgtccg
     5341 agccggggac gcttgtcgcg ttcgagtcgc cgaagcgggt cccggcggcg ctcgcgacac
     5401 tggccgaatt gacgcccgac cgcgaagcgg ccgtggcgcg cgagctcacg aaggcgcacg
     5461 aggaggtggt gcgcggcacc gccggggagt tggcggctcg gtacatggac gagccgccgc
     5521 gcggcgaggt ggtgctggtg atcggaccgc ttccgggccg aacagcggac gagccgggcg
     5581 aggaggcggt cgacgcgctc gcgcggctgg tcgaggcagg cgcaaagccg cgcgcggccg
     5641 cggcagtggt cgccgagctc acgggcggga gcgccaacgc gctctaccgg tcgctttcgt
     5701 cacggcgcag c
//
LOCUS       DASVXS010000086        12974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_517484, whole genome shotgun sequence.
ACCESSION   DASVXS010000086 DASVXS010000000
VERSION     DASVXS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517484"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1954
                     /locus_tag="VF752_05025"
     CDS             287..1954
                     /locus_tag="VF752_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX8854942.1"
                     /translation="MTVLRLNPSALDYAIIAVYFLVVLGIGAVARLTIKTDLDFFLSG
                     RSLPAWITGLAFIAANLGALEILGQAANGAQYGVPAVHYYWLGAVPAMVFLGIVMMPF
                     YYGAKVRSVPEYLRLRFNEATHAFNAGTFAIATVLISGVNLFALALIIHLMLGWSVSV
                     AILVAAAIVLVYITLGGLTSAIYNEVLQFFVIVASLLPLTIVALHSVGGVDGLKHRVQ
                     SFKPLADPALHAWKGLAIGHVSNPLASDWVGLVFGLGFVLSFGYWTTNFAEVQRALSA
                     RNMSAARRTPLIGAYPKIFLPAIIIVPGLIAAVTVPGLGSSSTNLQYNTAIPHLIGNF
                     LPEGMLGIAITGLLAAFMAGVAANVTSFNTVVTYDLIQSYFVKNRDSDYYLRAGRFVT
                     IAGVLISVATAFIAKGYSNIMNYIQTLFSIFNAPLFATFIIAMFWRRATAWGGFWSLV
                     AGTAAAYAVNRLNAYGLIFHFGSPLSASFWQAIIAFLADAVVLVLVSLVTQPKSPERL
                     RGLVWGLTRKAEPEENADPRDKLWWRSPALLGGVAIALLAALNIIFI"
     gene            1967..2299
                     /locus_tag="VF752_05030"
     CDS             1967..2299
                     /locus_tag="VF752_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854943.1"
                     /translation="MADEINPLPPTGATDEEEQRSARMANRYDIRRLIGGVFLLYGVV
                     LTVLGLFGSSHVKNKAAGINIDLWTGLGMLVFAAFMIGWAVLRPVQPEPPETRGQGSG
                     RIRRAPAT"
     gene            2332..3651
                     /locus_tag="VF752_05035"
     CDS             2332..3651
                     /locus_tag="VF752_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX8854944.1"
                     /translation="MTTHPGLAAAEEKMERGGVSDAARAAFARMYEQLVSGDAFELPG
                     DELEPARDVQRLEELPGADEAALDSVVVVKLNGGLGTSMGLDAPKSLIEVKPGHTFLD
                     VIVRQLLALRQRHRARLPLVLMNSYSTRDATLAALERYADLAVDVPLDFLQSREPKLR
                     ASDLEPIEWPDDPELEWNPPGHGDIYPSLIGSGMLEALLDRGYEYAFVSNVDNLGAVL
                     DPRVVAFVAAERRPFVMEVVEGTEADRKGGHIARRDGRLLLRESAQVPEGDDSFGDFQ
                     RWRYYNTNNLWLHLRTLAENGPPPLPLIVNRKTVDPRDSSTPEVLQLETAMGAALGTP
                     DGAEVVCVPRTRFAPVKTTNDLLVVRSDAYVLHEDDALLEPAPERAGAPPPVVDLDPS
                     FYKLLPDFESRFPAGPPSLARAERLTVRGDVTFGAGTVVSGAAEVVA"
     gene            3648..4697
                     /locus_tag="VF752_05040"
     CDS             3648..4697
                     /locus_tag="VF752_05040"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX8854945.1"
                     /translation="MTPSRVVAFAPGRANLIGEHTDYNDGLSLPFAIEQGVRVTAVPS
                     AAADLVVRSVDHHQEQQFPLTGLPTARFGDWRDFVRGAIGELSAAGFELRGGELEIDG
                     SVPEGGGLSSSAALEVALCMALIALSGAPEPTDRIALSRLCSRIENQWVGARTGLLDQ
                     LASLLGTEEHALRIDFRTLETRSVPLHLGSWRLITIDSCEPHSLAASGYNERRAECER
                     ARGLLGLESLRDARIEDLERLPDPLGRRVLHVIEENARVDAAVTALQSDDLAALAPLL
                     DASHRSLRDLFDASTPAVERAVARVLAAGAAGARMMGGGFGGQVIALMPPGTEVPERA
                     REVAPAAGARLLEAV"
     gene            complement(4918..6117)
                     /locus_tag="VF752_05045"
     CDS             complement(4918..6117)
                     /locus_tag="VF752_05045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX8854946.1"
                     /translation="MNLHDAFLGFGLALVIVWVMTPVAAAIAWRVGAIDEPRERGLHQ
                     FPTPRLGGLAILIGVEVAGWIWLPHTQETRGILIGAAVIALVGALDDLFELSADVKLA
                     GQILAAIIPVAMNVRVDTMTLPFLGHIDLGWTSYPLTVIGIVACMNAVNFIDGVDGLA
                     AGVCTIAAITFGTIALSLDRDAAGVLALIVAGSCLGYLRHGFHPASIFLGDSGSNLLG
                     YLLAAIAVQGALKTNAVIALAFPLVILAVPILDSSFVIAKRVKYGRPVYKADSWHFHH
                     RFANIGFSQRRTAVYIYLWTLSLGMLALALRFVPYTDDQGHWDLRWSLVMAGFGLLAV
                     AASVYLVLVLEILKLRRFRQFQARRAALAEGGPEPEPEHVDARVAHELETGEFEAVDP
                     AKLQQRE"
     gene            complement(6170..8074)
                     /gene="asnB"
                     /locus_tag="VF752_05050"
     CDS             complement(6170..8074)
                     /gene="asnB"
                     /locus_tag="VF752_05050"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8854947.1"
                     /translation="MCGICGLASLSGDPVDAETLSAMNATLVHRGPDDEGYHREGSVG
                     LAARRLSIIDLEGGHQPISNEDGTVTVVQNGEIYNHRGLRGDLERRGHRFATRCDTEV
                     LAHLYEERGARFVEDLRGMFAIALWDARERRLLLARDRFGIKPLYYRLEGGTLSFASE
                     LKALLAQPEFSRDVDVEALEAFLAFNSIPAPLTIFREARKLPAGHFLVLQGGRCAIER
                     FARPEPAPASEVRTEGMDELAEELRGCLRDSVRAHLVSDVPVGVLLSGGIDSSALAAL
                     AAQESGERVSTFSIGFEERGFNELELARLVAKRYDTDHHELVVRPDAADLLPKLAEAF
                     DEPFADSSALPTYLVSQLAADHVKVAMSGEGGDELFGGYYTYVADLLAPRVGRIASAL
                     RPLVDLLPSTSGKASFDYKAKRFARGAHLPPLERHHAWKEIFSPEARAELFAHHRRST
                     VDPVDVYRARWAETEGAELLARLQDVDLGIYLVDDLLVKTDRASMAHSLEARVPFMDT
                     VVADFAMRLPTEMKVKRLEKKRLLRAAVRPLLPEEIYSAKKQGFSIPAAAWLRGELEP
                     FARDVLSPDNVRRQGFFDPRAVTSLIDRHVARREDLSRQIWGLLSFSLWYERYARGPE
                     PTRPTRRVAA"
     gene            complement(8077..8796)
                     /locus_tag="VF752_05055"
     CDS             complement(8077..8796)
                     /locus_tag="VF752_05055"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8854948.1"
                     /translation="MSVKQKVPTSSDTAELQAAITPAGSLAERYARMAHEGFERRAVD
                     PVLRALDVVMAGTMLVLTSPIMGVAALAIYADSGKPALYRGRRVGRAGRIFTMLKFRT
                     LELDAESRLGPYLGPQLTDLTNAEVTRVGKWLRRTHVDELPQLWNVVRGDMSLVGPRP
                     IRPRFFEELCEQIPQYWQRLVVRPGMTGLAQIRIEREMTWSEKLAHDFEFIADRSVGL
                     YLSVIAETIARVLRRIVGGGD"
     gene            complement(8793..9800)
                     /locus_tag="VF752_05060"
     CDS             complement(8793..9800)
                     /locus_tag="VF752_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX8854949.1"
                     /translation="MRIWIDMTAPAHVLVFRPIIERLRRQGHEVEITARDYAQTLELL
                     ELHGIDHTHFGRHGGATRAKKVAALVSRTRAMRRFGKGKHFDLALAHGSNDLALAASS
                     LRIPAADMFDYEFATAQHNVGCRLARRVVTPDSIPPDRLERYGAGPDKLVQYPGLKEE
                     YYLADFEPNESVMDELGVNRERVTVVVRPPPDVSLYHRKSNKLFPAVLDRLGRDDDVH
                     AIVLPRTQEQRMYVKALGLPSVIVPDHAVDAQSLIALADLVVSAGGTMNREAVALGTP
                     VYTTYGGRLGGVDEWLIREGRLRPLTNARAIELQKRGTDGARTRRDPAILVNILLGTP
                     R"
     gene            complement(9871..10788)
                     /locus_tag="VF752_05065"
     CDS             complement(9871..10788)
                     /locus_tag="VF752_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8854950.1"
                     /translation="MHSHHHVHAHPHGSHHENKSLMVVALAINVALLAIGVAGWIAFD
                     SVALLADAGHVLSDVGAIALGVFAATVAARPPTAKRTFGYARTEILAALANGVLLVAV
                     AVLVFVEAIGRLNGSPDVGGGGVLAVGLIGLAGNAVATAVLQRGDREDINLEGVLRHS
                     AADALGSLGVIVAGAVVLATGWDQADAVAGMLIGVLVLLGSWRLISEPVNVLMEGAPP
                     GVNANEVGEAMARVEGVREVHDLHVWTVTSGFPALAAHVIVEPAHDPDEVRRRVEAVL
                     KERFGIEHTTLQAMAERLLELEDRLPGNR"
     gene            10856..12061
                     /locus_tag="VF752_05070"
     CDS             10856..12061
                     /locus_tag="VF752_05070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8854951.1"
                     /translation="MPAPERNQRAAGLDGLRGLAALTVLTFHVWLYHFPDPANVRRDS
                     FGDYVASDLRLGLILFFVLSGYLLYRGFARAALRREGMVDLRSYVARRAARILPAYYI
                     ALVGTFALLWDAKGVTGLRLPDESDLPLFAIFAQNYNPHTMMSFNPVTWTLCVEILFY
                     ALLPLLGLLAYRFFAGSARRQLGMLLALVATGVFWNWLVYSQGWNMVASKALIAYLPY
                     FAAGMLVALLAEWRLARTGEPLRFGPLATAAIALAGFGAVVANSLWHISDAGIRLQAI
                     NLTMRDLPAGTGFGLLVLAVVAGTGPAIAWSRFRPLVAVGVVSYALYLWHLPLMLFEE
                     RIGAVPGPLPLRWIALLVPSLAVAAASWLLVEKRVLAKVHRVDRRRRERRTKPTSTGR
                     PALESHAAP"
     gene            complement(12070..>12974)
                     /locus_tag="VF752_05075"
     CDS             complement(12070..>12974)
                     /locus_tag="VF752_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8854952.1"
                     /translation="SDADAPMTAAAFARQLRQQATARARRARRELILLVPLLVATILA
                     YEYREDLFGVDTPIRVAAAIVLVIIGWAFARALGRALGPSLFRRMDAGTAGTVGFLIR
                     LVTIAFAVLFALRVAGVQPQTVAVGGALTAVVFGLAAQQTLGNLIAGTVLISARPFRV
                     GDRVRFQAGGLAGQVEGVVTSLGLLYTTLAHGEDSIMVPNSVVLGAAVVPLREPAGVD
                     VRARLRPGIKPSEVHDLIEHNLTVPIRDRPHVGLEEVDGDQVVVRVTATPASNADGGR
                     LADDVLEVLHQVTAEPEQLYPSSG"
BASE COUNT         1931 a         4539 c         4494 g         2010 t
ORIGIN      
        1 tccagctgcg gtttagtcgg ctgcaaacct tcgctctgcg gcgtcctcgg gcgctcgctt
       61 ggctatcgcc aagcttcggc cctgcgtcct tgcatagctc ggttttcgcc ggctaacgcg
      121 agctgtaggc cgggaggagt ccgacgcgta cgctcggaac ctgcgcggcg cgccggtcgt
      181 ccgccgcgcg acggatcttt agccgcccgc cgtaccgcca ggggagtacg gaaaagagtc
      241 aaccgagggc cgacgacgtt ccgctccccg cggagcacga tgctgggtga ccgtgcttcg
      301 cctgaacccg agcgcactcg actacgcgat catcgcggtc tacttcctgg tggtcctcgg
      361 gatcggcgcg gtagcgaggc tcacgatcaa gactgatctc gacttcttcc tgtcggggcg
      421 gtcgctgccc gcgtggataa cggggcttgc gttcatcgcc gcgaacctcg gggcgctcga
      481 gatcctgggc caggcggcca acggggcgca gtacggcgtg ccggccgtcc actactactg
      541 gctcggcgcg gtgcccgcga tggtcttcct cggcatcgtg atgatgccgt tctactacgg
      601 ggcgaaggtc cgctccgtgc ccgaatacct caggctgcgc ttcaacgagg caacgcacgc
      661 gttcaacgca ggcaccttcg cgatcgcgac ggtcctcatc tcgggcgtga acctgttcgc
      721 gctggctctg atcatccacc tcatgctggg ctggtcggtg tcggtggcga tcctcgtcgc
      781 cgcggccatc gtcctcgtgt acatcacgct cggcggcctc acgtcggcga tctacaacga
      841 ggtgctccag ttcttcgtga tcgtggcgag tctgctgccg ctcacgatcg tggcgctgca
      901 cagcgtcggc ggagtcgacg gcctcaagca ccgcgttcag agcttcaaac cgctcgcgga
      961 tccggcgttg cacgcttgga aaggcctcgc catcgggcac gtgagcaacc cgctcgcgtc
     1021 cgattgggtg ggactcgtgt tcgggctcgg cttcgtgctc agcttcggct actggacgac
     1081 gaacttcgcc gaggtgcaac gcgcgctgtc ggcgcggaac atgtcggccg cgcgccgcac
     1141 gccgctgatc ggggcctacc cgaagatctt cctaccggcg atcatcatcg tcccgggcct
     1201 gatcgcggcg gtgaccgtcc cgggtctcgg tagcagtagc acgaaccttc agtacaacac
     1261 cgcgatccct cacctgatcg gcaacttcct ccccgagggc atgctgggga tcgctatcac
     1321 cgggctgctc gccgccttca tggcaggggt ggctgcgaac gtcacctcgt tcaacacggt
     1381 cgtgacttac gacctgatcc agtcctactt cgtgaagaac cgggactcgg attactacct
     1441 gcgcgcgggc cggttcgtga cgatcgccgg cgttctgatc tcggtggcca cggcgttcat
     1501 cgccaagggc tactcgaaca tcatgaacta catccagacg ctgttctcga tcttcaacgc
     1561 gccgctgttc gcgacgttca tcatcgcgat gttctggcgc cgcgcgacgg cgtggggagg
     1621 gttctggagc ctagtggcgg ggactgcggc cgcttatgcg gtcaaccggc tgaacgcata
     1681 cggactgatc ttccacttcg gctcgccgct gagcgcgagc ttctggcagg cgatcatcgc
     1741 gttcctggcc gacgccgtag tgctcgtgct cgtttcgctc gtcacccagc cgaaatcccc
     1801 cgagcgcctg cgcggcctcg tgtggggcct cacgcgcaag gcggagcccg aggagaacgc
     1861 cgacccccgc gacaagctgt ggtggcgctc gccggcgctc ctcggcgggg tcgcaatcgc
     1921 gcttctcgcc gcgctcaaca tcattttcat ctaggaggca gcccgcatgg ccgacgaaat
     1981 caatccgctg ccgcccaccg gcgccactga cgaggaagag cagcgctcgg ctcgtatggc
     2041 caaccgctac gacatccgcc ggttgatcgg cggcgtgttc ctcctctacg gcgtcgtcct
     2101 cacggtgctc gggctgttcg gcagctcgca cgtcaagaac aaggccgccg ggatcaacat
     2161 cgatctgtgg accggactgg gcatgctcgt gttcgccgcg ttcatgatcg gctgggcggt
     2221 gctacggccc gtgcagccgg agccgcccga gacgcgcggc cagggctcgg ggcggattcg
     2281 ccgcgcgccg gcaacgtgag tgtgcgcgcg ggcccgggta cgcgcgcgcc tatgacgaca
     2341 catccgggac tggccgcggc cgaggagaag atggagcgcg ggggcgtctc ggacgccgcg
     2401 cgcgccgcgt tcgcgcggat gtacgagcag ctcgtgagcg gagacgcctt cgagctgccg
     2461 ggtgacgagc tcgagccggc tcgcgacgta cagcgactcg aagagctgcc cggcgctgac
     2521 gaggcggcgc tcgacagcgt ggtcgtcgtg aagttgaacg gcggcctcgg cacgagcatg
     2581 ggcctggacg cgccgaagtc gctgatcgag gtcaagccgg gtcacacctt cctcgacgtg
     2641 atcgtccgcc agctgctcgc gctccggcag cgtcaccggg cgcggctgcc gctggtgctg
     2701 atgaactcgt actcgacgag ggacgcaacg ctggcagcgc tcgagcgcta cgccgacctc
     2761 gcggtcgacg tgcccctcga cttcctccaa agccgtgagc cgaagctccg tgcgagtgac
     2821 ctcgagccga tcgagtggcc tgatgatccc gagctcgagt ggaacccgcc gggccatgga
     2881 gacatctatc cctcgctgat cgggtcggga atgctcgagg cgctactcga tcggggctac
     2941 gagtacgcat tcgtctcgaa cgtggacaac ctcggcgccg tgctcgaccc gcgtgtggtc
     3001 gccttcgtgg cagccgagcg gcggccgttc gtgatggagg tggtggaggg gacggaggcg
     3061 gatcgcaagg gcggtcacat cgcgcggcgc gatggccgcc tgctgctgcg cgagtccgcg
     3121 caggtacccg agggcgacga ctcgttcggc gacttccagc gctggcgcta ctacaacacc
     3181 aacaatctct ggcttcactt gcggacgctc gccgagaatg gcccgccgcc gctcccgctc
     3241 atcgtgaacc gtaagacggt cgacccgagg gattcctcga cgcccgaggt actgcagctc
     3301 gagaccgcga tgggggccgc gcttggcacg cccgacggcg ccgaggtggt gtgcgtgccg
     3361 cgtacccgct tcgcgccggt gaagacgacg aacgacctgc tggtcgtgcg ctcggacgcg
     3421 tacgtcctgc acgaggacga cgcgctgctc gaacccgcgc ccgaacgagc cggtgcaccg
     3481 ccgccggtcg tcgatctcga cccgagcttc tacaagttgc tgcccgactt cgagtcgcgc
     3541 tttcccgccg gcccaccgtc gctcgcgcgc gccgagcgcc tcacggttcg cggtgacgtg
     3601 actttcggcg ccggcacggt cgtctcaggg gcggccgagg tggtggcttg acgcccagcc
     3661 gggtggtggc gttcgcgccg ggccgggcca acctcatcgg cgagcacacc gactacaacg
     3721 acgggctgag cctgccgttc gccatcgagc agggggtacg cgttacggct gtcccgagtg
     3781 ctgcggctga cctggtcgtc cggtccgtcg accaccacca ggagcagcag ttcccgctca
     3841 cgggcctacc gacagcgcgt ttcggggact ggcgggactt cgtgcgcggg gcgatcggcg
     3901 agctcagcgc ggccggtttc gaactgcgcg gcggcgagct agagatcgac ggcagcgtgc
     3961 cggagggagg cgggctctcc tcgtcggcgg cactggaggt cgcgctatgc atggcgctga
     4021 tagcgctgtc cggggcgccc gagcccaccg accgcatagc tctcagccgg ctgtgctcgc
     4081 gcatcgagaa ccagtgggtg ggggcgcgca ccgggctgct cgaccagttg gcatcgctcc
     4141 tcggcaccga ggagcacgct ctacggatcg acttccgcac actcgagacg cgatcggtgc
     4201 cgctccacct gggcagctgg cggctgatca cgatcgactc gtgcgagcca cactcgctcg
     4261 ccgcgtcggg ctacaacgag cggcgggccg agtgcgagcg agcgcgcggg ctgctcggcc
     4321 tcgagagcct gcgcgacgcg cgcatcgaag acctcgagcg gctgcccgac ccgcttggac
     4381 ggcgcgtcct ccacgtgatc gaggaaaacg cgcgcgtgga cgccgccgtc acggcgctcc
     4441 agtccgacga cctcgcggcg ctcgccccgc tgctcgacgc ctcgcaccgg agcctccgcg
     4501 atctgttcga cgcctccacc ccagcggtcg agcgcgcagt cgcgcgcgtt ctggccgcgg
     4561 gcgcggcagg cgcgcgcatg atgggcggcg gcttcggagg gcaggtgata gcgctcatgc
     4621 cgcccggaac ggaggtaccg gagcgcgctc gcgaggtcgc gcctgccgcg ggcgcgcggc
     4681 tcctcgaggc ggtgtgatcg gaagccgcga tcgcggagaa cgtcttagcc ctcgtggcgg
     4741 ccggcatgag caatcacgag atcgccgagc acttggtcgt gagccccgcg accgcgaaga
     4801 cgcacgtgag cagggcgctc cgcaagctgc gcctgcgcga ccgcgctcag ctcgtgtccg
     4861 tggcctacga gtgcgggctg gtggtcccgc gcgcgtaccg ctatgccggc cgtgtcccta
     4921 ttcgcgctgc tggagcttgg ccggatcaac tgcctcgaac tcgcccgtct ccagctcgtg
     4981 cgcaacgcgg gcgtcaacgt gttcaggctc cggctccggc ccgccctcgg cgagcgctgc
     5041 acggcgtgcc tggaactggc gaaaccgccg cagcttgagg atctccagca cgagcacgag
     5101 atagacggac gccgcgaccg cgagcagccc gaagcccgcc atgacgaggc tccagcgcag
     5161 gtcccagtgc ccttggtcgt cggtgtaagg gacgaagcgc agggccagcg cgagcattcc
     5221 gagcgaaagc gtccacaggt agatgtagac cgcggtgcgc cgctgcgaga agccgatgtt
     5281 cgcgaagcgg tggtggaagt gccaggagtc cgccttgtag accggcctcc cgtatttcac
     5341 gcgcttcgcg atcacgaagc tcgagtcgag gatcggaacg gccaggatca ccagcgggaa
     5401 cgcgagggcg atcacggcgt tggtcttcag ggccccctgc acggcgatcg cagcgagcag
     5461 gtagccgagc aggttcgacc ccgagtcccc gaggaagatc gacgctgggt ggaagccgtg
     5521 acgcaggtag cccaggcagc ttcccgcgac gatcagagca agtacgcccg cggcgtcgcg
     5581 gtcgagcgac agggcgatcg tgccgaacgt gatcgcagcg atcgtgcaca cgcccgcagc
     5641 aagcccgtca acgccgtcga tgaagttcac ggcattcatg caggccacga tcccgataac
     5701 ggtgagcggg taggacgtcc agccgaggtc gatgtggccg aggaacggca gcgtcatcgt
     5761 gtcgacgcgc acgttcattg ccaccgggat gatcgccgcg aggatctggc ccgccagctt
     5821 cacgtccgcc gacagctcga agaggtcgtc gagcgcgccg acgagggcga tcaccgccgc
     5881 gccgatcagg atgccgcgcg tctcctgcgt gtgcggcagc cagatccagc ccgccacctc
     5941 cacgccgatg aggatcgcca gcccgccgag gcggggcgtc gggaactggt gcagcccgcg
     6001 ctcccgcggc tcatcgatcg ctccgacgcg ccaggcgatc gccgcagcga ccggcgtcat
     6061 cacccagacg atcacgagcg cgaggccgaa gcccaggaat gcgtcgtgga ggttcacggg
     6121 cccaagggta gggttcgccg cggcagcgcg tgaacctctg cgcgccagac taggcggcca
     6181 cgcggcgggt ggggcgcgtg ggctcgggtc ctcgcgcgta gcgctcgtac cagagcgaga
     6241 aggagagcag cccccagatc tgccgcgaca ggtcctctcg gcgcgccacg tgccggtcga
     6301 tcagcgaggt cacggccctc gggtcgaaga agccctgacg gcggacgttg tccggggaga
     6361 gcacatcgcg cgcgaacggc tcgagctcac cgcgcagcca tgccgccgcg gggatcgaga
     6421 agccctgctt cttcgcgctg tagatctcct cgggcaggag cggccgcacc gccgccctga
     6481 gcaaccgctt cttctcgagg cgcttcacct tcatctcggt cggcagccgc atggcgaagt
     6541 cggcgacgac cgtgtccatg aacgggacac gtgcctcgag cgaatgcgcc atgcttgccc
     6601 ggtccgtctt cacgagcagg tcgtccacga ggtagatgcc gaggtccacg tcctgcagcc
     6661 gcgcaagcag ctcggctccc tcggtctcgg cccagcgagc gcgatagacg tcgaccgggt
     6721 cgacggtgct gcgtcggtgg tgcgcgaaca gctcggcccg agcctcgggg gagaagatct
     6781 ccttccacgc gtggtggcgc tcgagcggcg gcaggtgcgc gccgcgcgcg aagcgcttgg
     6841 ccttgtagtc gaagctcgcc ttgccggacg tgctcgggag cagatcgacc agcgggcgta
     6901 gcgccgacgc gatacgcccc acccgcggcg cgagcaggtc ggccacgtag gtgtagtagc
     6961 cgccgaacag ctcgtctccg ccctcgcccg acatcgccac cttcacgtgg tccgcggcga
     7021 gctgtgacac gagataggtg ggtagcgccg acgagtccgc gaacggctcg tcgaacgcct
     7081 ccgccaactt cggcagaagg tcggccgcgt ccggccggac gaccagctcg tggtggtcgg
     7141 tgtcgtagcg cttcgccacc aggcgcgcca gctcgagctc gttgaacccg cgctcctcga
     7201 agccgatcga gaaagtgctc actcgctcgc cggattcctg cgccgcgagc gccgcgaggg
     7261 cggaggagtc gatcccgccg gacagcagca cgccgaccgg cacgtcggac accagatgcg
     7321 cgcgcacgga gtcgcgcaga cagccgcgca gctcctccgc cagctcgtcc attccctcgg
     7381 tgcgcacctc ggacgccggc gccggctccg gacgcgcgaa gcgctcgatc gcgcagcggc
     7441 cgccctgaag cacgaggaag tggccggccg gcagcttgcg cgcctcgcgg aagatcgtga
     7501 gcggcgccgg aatcgagttg aacgcgagga acgcctcgag ggcctcgacg tcgacgtcgc
     7561 gcgagaactc cggctgggcc agcagcgcct tcagctccga ggcgaacgag agcgtgccgc
     7621 cctcgagccg gtagtaaagg ggcttgatcc cgaagcggtc gcgcgcgagc aggaggcgcc
     7681 gctcgcgagc gtcccagagc gcgatcgcga acatgccgcg gaggtcctcg acgaagcgcg
     7741 cgccgcgctc ctcgtacagg tgcgcgagca cctcggtgtc gcagcgcgtg gcgaaccggt
     7801 gccctcgccg ctcgaggtcc cctcgcagcc cgcggtggtt gtagatctcg ccgttctgga
     7861 cgaccgtcac cgtgccgtcc tcgttcgaaa tcggctggtg gccgccctcg aggtcgatga
     7921 tcgagaggcg ccgggcggcg aggcccacgg acccctcgcg gtggtagccc tcgtcgtcgg
     7981 gaccccggtg caccagggtc gcgttcatcg cggagagcgt ctcagcgtcg accgggtcgc
     8041 ccgagagcga cgcgaggccg cagatcccgc acacgcctag tcaccgccac cgacgatgcg
     8101 gcggagcacg cgcgcgatgg tctccgcaat cacgctgagg tacagcccca cggagcgatc
     8161 ggcgatgaac tcgaagtcgt gcgcgagctt ctccgaccag gtcatctcac gctcgatccg
     8221 gatctgcgcc agccccgtca tgcccgggcg caccaccagc cgctgccagt actgcgggat
     8281 ctgttcgcac agctcctcga agaagcgcgg gcggatcggc cgcggcccga ccaggctcat
     8341 gtcgccgcgc accacgttcc agagctgcgg cagctcgtcc acatgcgtgc ggcggagcca
     8401 cttgcccacg cgtgtgacct cggcgttcgt gaggtccgtg agctgcgggc ccaggtaggg
     8461 gccgaggcgc gactcggcgt cgagctccag cgtgcggaac ttgagcatcg tgaagatgcg
     8521 gcccgcgcga cccacgcgcc ggccgcggta gagcgccggc ttgcccgagt cggcgtagat
     8581 cgcgagtgcg gccaccccca tgatcggcga ggtcaggaca agcatcgtcc cggccatcac
     8641 gacgtcgagc gcgcggagta cggggtcgac cgcgcggcgc tcgaagccct cgtgggccat
     8701 gcgcgcgtag cgctccgcga gcgagccggc tggggtgatc gcggcctgta gctccgccgt
     8761 gtcgctcgag gttggaacct tttgcttcac gctcatcgag gtgtgccgag cagaatgttg
     8821 acgagtatgg cggggtcgcg gcgcgtgcgt gcgccgtccg tgccgcgctt ctgaagctct
     8881 atggctcggg cgttcgtcag cgggcgcagc cgtccttcac ggatgagcca ctcgtccacc
     8941 ccgccgagcc ggccaccgta ggtcgtgtag accggggttc cgagggcgac ggcctcgcgg
     9001 ttcatggtgc cgccggcgga gaccacgagg tcggcgagcg cgatcaggct ctgggcgtcg
     9061 accgcgtggt cgggcacgat caccgacggc agcccgagcg ccttcacgta catgcgctgt
     9121 tcctgcgtgc gcggcagcac gatcgcgtgc acatcgtcgt cgcggccgag ccggtcgagc
     9181 accgcaggga agagcttgtt cgacttgcgg tggtagaggg acacatcggg cgggggcctc
     9241 accacgactg tcacgcgctc gcggttgacc ccgagctcgt ccatcacgga ctcgttcggc
     9301 tcgaagtctg cgaggtagta ctcctccttc agccctgggt actgcacgag cttgtccgga
     9361 cccgcgccgt accgctcgag gcgatcgggc gggatcgagt ccggcgtgac gacccggcgt
     9421 gcgagccggc aacccacgtt gtgctgggcg gtcgcgaact cgtagtcgaa catgtccgcc
     9481 gcgggtatcc gcagcgacga ggcggcgagt gcgaggtcgt tcgagccatg ggcgagcgcg
     9541 agatcgaagt gcttgccctt gccgaagcga cgcatcgccc gcgtgcgcga cacgagagcg
     9601 gcgaccttct tcgctcgcgt ggcaccgccg tggcggccga agtgggtgtg gtcgatgccg
     9661 tggagctcga gcagctcgag cgtctgagcg tagtcacgcg cagtgatctc gacctcgtgc
     9721 ccctgccggc gcaggcgctc gatgatcggc cggaacacga gcacgtgcgc cggggcggtc
     9781 atgtcgatcc agatcctcat gggagggagc ccgggccgca ctcggtgccc gggccggcgg
     9841 ccgactcaat atagagaggg agccgggcgc tcagcggttg cccgggagcc ggtcctcgag
     9901 ttcgagcagc cgctcggcca tggcctggag cgtggtgtgt tcgatgccga agcgctcttt
     9961 caggaccgcc tcgacccgcc ggcgcacctc gtccgggtcg tgcgccggct ccacgatcac
    10021 gtgcgccgcg agcgccggga agccgctcgt gacggtccat acgtgcaggt cgtgtacctc
    10081 gcgcacgcct tctacgcgcg ccatcgcctc gcccacctcg ttcgcgttca cgccaggcgg
    10141 cgcgccctcc atcagcacgt tgacgggctc ggagatgagg cgccacgagc cgagcagcac
    10201 gagcacgccg atcaacatgc cggccaccgc gtcggcctgg tcccagccgg tcgcgagcac
    10261 gactgcgccg gcgacgatca cgccgagcga gccgagcgcg tcggccgccg agtgacggag
    10321 cacgccctcg aggttgatgt cctcgcggtc gccgcgctgg agcaccgcgg tcgccactgc
    10381 gttcccggcc aacccgatga ggccgacggc gagcacgcca cctccgccga cgtccggcga
    10441 gccgttcagc cgccctatcg cctccacgaa caccaggacg gcgacggcca cgagtagcac
    10501 tccgttcgcc agcgccgcga ggatctcggt gcgggcataa ccgaatgtgc gcttcgcggt
    10561 gggcggacgt gccgccaccg tcgctgcgaa gactcccagc gcgatcgcgc ccacgtccga
    10621 gagcacgtgg cccgcgtctg cgagcagcgc cacggagtcg aaggcgatcc agcccgccac
    10681 tccgatcgcg agcagtgcga cgttgatcgc gagcgcgacc accatcaggc tcttgttttc
    10741 gtgatggcta ccgtgcgggt gggcgtgcac gtggtgatga gagtgcatca ccgcctattg
    10801 cagcacccgc accccttgga gcgtcgcgtt gaacctagtt gggtacaaac ctcgcatgcc
    10861 tgcgcccgaa cgcaaccagc gtgcggcggg gctcgacggc ctgcgcggcc tcgcggccct
    10921 caccgtgctc acgttccacg tgtggctgta ccacttcccg gacccggcga acgtccggcg
    10981 cgacagcttc ggcgactacg tggcgtccga tctgcggctc ggcctgatcc tcttcttcgt
    11041 cctgtccggc tacctgctct accgcggctt cgctcgcgcc gcgctccgtc gcgagggcat
    11101 ggtcgacctg cgctcgtacg tggcccgtcg cgccgcgcgc atcctgcccg cctactacat
    11161 cgccttggtc ggcaccttcg cgctgctgtg ggacgcgaaa ggcgtgaccg gcctgcggct
    11221 gcctgacgag agcgacctgc cgctgttcgc gatcttcgcc cagaactaca acccgcacac
    11281 gatgatgtcg ttcaacccgg tgacctggac gctgtgcgtg gagatcctct tctacgcact
    11341 gctgccgctg ctcggcctgc tcgcgtatcg cttcttcgcc gggagcgcgc ggcgtcagct
    11401 cggcatgctc ctcgcgctcg tcgccaccgg cgttttctgg aactggctcg tgtactcgca
    11461 gggctggaac atggtcgcgt cgaaggcgct gatcgcgtac ctgccgtact tcgcggcggg
    11521 catgctcgtg gccctgctgg ccgagtggcg actcgcgcgc accggcgagc cgctccgttt
    11581 cgggcccctc gcgacggcgg cgatcgcgct cgcgggcttc ggggctgtgg tcgcgaacag
    11641 cctgtggcac atcagcgacg ccggcatccg gcttcaggcg atcaacctca ccatgcgcga
    11701 tctgcccgcc ggcaccgggt tcggcctgct cgtgctcgca gtcgtggccg ggaccgggcc
    11761 ggccatcgcg tggagccgct tccggccact cgtcgcggtg ggcgtcgtgt cgtacgcgtt
    11821 gtacctctgg catctcccgc tgatgctgtt cgaggagcgg atcggcgcgg tgccgggccc
    11881 gctgccgctg aggtggatcg ccctgctggt gccatcgctc gcggtcgcgg ccgcgagctg
    11941 gctgctcgtc gagaagcgcg tgctggcgaa ggtgcaccgg gtcgatcgcc gccgccgcga
    12001 gcgtcggacc aagccgacct ccaccggtcg tccggcgctc gaaagccacg cagctccctg
    12061 agcgcgggct cagccggagg acgggtacag ctgctcgggc tctgctgtca cctggtgcag
    12121 cacctcgagc acgtcgtccg cgagccttcc gccgtcggcg ttcgacgccg gcgtggccgt
    12181 cacgcgaacc accacctgat cgccatcgac ctcttcgagg ccgacgtgag gccggtcgcg
    12241 gatcggaacg gtgaggttgt gctcgatcag atcgtgcacc tcgctcggct tgatgccggg
    12301 ccgtaggcgc gcgcgcacgt ccacaccggc cggctcgcgc agcggcacga cggccgcgcc
    12361 gagcactacc gagttcggca ccatgatgct gtcctcgccg tgcgcgagcg tggtgtagag
    12421 caggccgagg ctcgtgacga cgccctcgac ctggccggcg aggccgccag cctgaaagcg
    12481 cacgcggtcc ccgacgcgga acgggcgcgc gctgatgagc acggtgccgg cgatgaggtt
    12541 gccgagcgtc tgctgagctg cgagaccgaa gacgacggcc gtgagcgcgc caccaacggc
    12601 gacggtctgc ggctgcacac ccgccacccg gagggcgaac aagaccgcga acgcgatcgt
    12661 gaccagtcgg atcaggaacc cgaccgtgcc cgccgtgccc gcgtccatcc gccggaagag
    12721 cgacggaccc agcgcgcggc cgagcgcccg cgcgaacgcc cagccgatga tcaccagcac
    12781 gatggccgcc gcgaccctga tcggagtgtc gacgccgaac aagtcctccc ggtactcgta
    12841 ggcgaggatc gtcgcgacca gcagcggcac aagcaggatg agctcgcgcc gcgcgcgccg
    12901 ggcgcgtgcc gtcgcctgct gcctcagctg ccgggcgaac gcggcggccg tcatcggcgc
    12961 gtccgcgtcg cttg
//
LOCUS       DASVXS010000087         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_223538, whole genome shotgun sequence.
ACCESSION   DASVXS010000087 DASVXS010000000
VERSION     DASVXS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223538"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1020)
                     /gene="bioB"
                     /locus_tag="VF752_05080"
     CDS             complement(4..1020)
                     /gene="bioB"
                     /locus_tag="VF752_05080"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX8854953.1"
                     /translation="MPPLITHEEALALGEIEDHAEIEALVERAFEARTERFGDSTDLC
                     SLVNAKSGGCAEDCGFCAQSRFAEADTPMHAMMSPEQILEHARAAEAAGAHRFCMVTQ
                     GQGLSKRDFQNIVEGAKLVAEHTSLKRCASVGHMSVDRATALREAGIQRVHHNVETAE
                     SYYDEVSTTVRYEGRIRTIEAVKEAGLETCVGGILNLGESREQRVEMAFQLASINPTS
                     VPINLLNPRPGTKFGDRELIDPWEAVKWIAIFRLILPDALFRLCGGRVENLGELEPLA
                     VKAGLNGVMMGNFLTTLGSEPEDDRRMFEQLGLNTARQPDNGSSPRPDNRSGWLEGES
                     PARA"
     gene            1199..>1757
                     /locus_tag="VF752_05085"
     CDS             1199..>1757
                     /locus_tag="VF752_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854954.1"
                     /translation="MALALPEAWESFKYRTGSARVRARQRHPNRMRSIRYAVPALVED
                     ATWRMRTGRVAREHRSPAQIRRRRRLVPLVAVLAVGAAFAAYTLASTSDKPKGGTKVA
                     QPVVAPQAAHPAVVPAQRAVTEPVVPKVVHHKRSHAAHHRAHKRHARHIAAAPAPAPT
                     PVETTPKRTPVKHKSAPKRETPVYRP"
BASE COUNT          266 a          615 c          601 g          275 t
ORIGIN      
        1 atcctaagcc cgcgcgggcg actcgccctc gagccagccc gagcggttgt cgggcctcgg
       61 gctcgagccg ttgtccggct ggcgggcggt gttcaagccg agctgctcga acatgcggcg
      121 gtcatcctcg ggctcggagc cgagcgtggt gaggaagttg cccatcatca cgccgttcag
      181 ccccgccttg accgcgagcg gctcgagctc gccgaggttc tcgacgcggc cgccgcagag
      241 ccggaacagg gcgtctggga ggatcaggcg gaagatcgcg atccacttga cagcctccca
      301 cgggtcgatc agctcacggt ctccgaactt ggtgcccggg cgggggttca gcaggttgat
      361 cggcacgctc gtggggttga tcgacgcgag ctggaaagcc atctcgacgc gctgctcacg
      421 cgactccccg aggttcagaa tgccgcccac gcaggtctcg agcccggcct ccttgaccgc
      481 ctcgatcgtg cgaatgcggc cctcgtagcg cacggtcgtg gagacctcgt cgtagtagct
      541 ctcggcggtc tccacgttgt ggtgcacgcg ctggatgccc gcctccctga gtgccgtcgc
      601 acgatcgacc gacatgtgcc cgaccgatgc gcagcgcttc aggctggtgt gctccgccac
      661 gagcttcgcg ccctcgacga tgttctggaa gtcgcgcttc gagagcccct gcccttgagt
      721 gaccatgcag aagcgatgcg cgcccgcggc ctcggccgcc cgcgcgtgct cgaggatctg
      781 ctcggggctc atcatcgcgt gcatcggcgt gtcggcctcg gcgaagcgcg actgggcgca
      841 gaagccgcag tcctccgcgc agccgcctga cttcgcgttc accagcgagc agaggtcggt
      901 ggagtcgccg aaccgctcgg tgcgggcctc gaaagcgcgc tcgaccagtg cctcgatctc
      961 ggcatggtcc tctatctcac cgagggctag ggcctcctcg tgggtgatga gcggcggcaa
     1021 gcggggcgac ctccttggcg gaaaatgttc cgctcggacg gtaggcattg cgtcggacgg
     1081 ccaccctttc ggccgtttta gtgcatgatg ggtatgtaag gcgggctcgc tcttgctcta
     1141 cgaagcacca accccccgaa ggcacccccc gaggtccggt cccggccggg tcaggcggat
     1201 ggcgcttgca ttgcccgagg cctgggagtc gttcaagtac cggaccgggt ccgcgcgcgt
     1261 gcgcgcgcgt cagcggcacc cgaaccgtat gaggagcatc cgctatgccg ttccggcgct
     1321 ggtcgaggac gcgacctggc gcatgcggac cggccgcgtg gcacgggagc accgcagccc
     1381 ggctcagatc aggcgccgcc gccggctggt tccgctcgtc gcggtgctcg ctgtgggcgc
     1441 cgctttcgcc gcctacacat tggcctcgac cagcgacaag ccgaaggggg gaacgaaggt
     1501 cgcccagccg gtcgtcgcgc cgcaggcggc tcacccggcg gtcgttccgg cccagagggc
     1561 ggtgaccgag ccggtcgtgc ccaaggtggt tcaccacaaa cgcagccacg ccgctcacca
     1621 ccgtgcacac aagcgtcacg caaggcacat cgcagcggca ccggcgcccg ctcccacacc
     1681 cgtcgagacc actccgaaga ggacgccggt gaagcacaag agcgcgccga agcgagagac
     1741 gccggtgtat cggcccc
//
LOCUS       DASVXS010000088        12402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1072712, whole genome shotgun sequence.
ACCESSION   DASVXS010000088 DASVXS010000000
VERSION     DASVXS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12402
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072712"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF752_05090"
     CDS             <1..381
                     /locus_tag="VF752_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8854955.1"
                     /translation="AEPDGLLLPFLLHPTPELLERQSRLRSTHTSLISEILNLLSGRS
                     PAARPEDAEPLQEALSETELRVLRYLPTNLRGPEIASELFVSLNTIRAHLRNIYAKLG
                     VHSRADAVKRARELGLLAPSSLKR"
     gene            507..1502
                     /locus_tag="VF752_05095"
     CDS             507..1502
                     /locus_tag="VF752_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX8854956.1"
                     /translation="MSHGEEQRQMEAIVIEASGPPAQSLTYRSEPVPEPGPGEVLVEV
                     HAAAVNPLDIANILGLLGTPLPKIPGVDFAGVVVSDGDSLGEEVWGSGPALGMALDGN
                     RAGTHARCVAIPATWLSRKPERLTMAAAAAVGRSHWAAWETLINMMEITPGETILITG
                     GAGLVGQAATAIARWRGAEVIIADRRKPDDVMQFIDTTSSDIREAVLELTDGRGADLV
                     LDTVGGELFVPALRSLRYDGRMVGLHGGTEPVTFDLNEIYNRQLHVTGLATVFYDGAH
                     VARIFDQFRALFDHGLLDPPAVKTWPLEKAAEAYQTVLDGSAGIKQVLLPAGRVA"
     gene            1514..2149
                     /locus_tag="VF752_05100"
     CDS             1514..2149
                     /locus_tag="VF752_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8854957.1"
                     /translation="MSSDPALLPDSMRAPGLTQFAAPTAALATVRLRLPTAAQGEPLG
                     PSSSVDPFTPQELAAWRGLLELHAQVIRQLDVQLHALHGLTLSQCEVLILLDDAPGER
                     MRMTELAAGVLLSRSACTRLVDRLETLGYVTRRAATDDRRGLYAKLTDTGRHVITPAR
                     ATHREGVRAAFLDRLRTTDQVALDDIWARVRGALPSSDPTGRTVSQTARKP"
     gene            complement(2404..3108)
                     /locus_tag="VF752_05105"
     CDS             complement(2404..3108)
                     /locus_tag="VF752_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8854958.1"
                     /translation="MESVLLDVVGQRRSPATMPGYHPGRPPRNKGEHYPADPPTVEEI
                     VAVMRAVGDRAEGKRLRALIMLLWRAGLRISEALALQESDLDAARGAILVRHGKGGKR
                     REVGMDRWAWAQLEPWLEKRRELPIGALLCVIHGPTAGRHWEASAARKQLHHAAAAAG
                     VRRRFAPHQLRHAHAVEMAHEGVPLVVIQRQLGHANLGITSVYLQGIDSAEIIDTVHG
                     RPSPMISASTGLEIAP"
     gene            complement(3298..4758)
                     /locus_tag="VF752_05110"
     CDS             complement(3298..4758)
                     /locus_tag="VF752_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8854959.1"
                     /translation="MGAQDEDSLREAGKLPKGRLARTARVGGLVTGQGLRWAGMRTAN
                     RIRTPERATAAQKERTAALVQQLVDQLGQMRGAAMKVGQMISMVEFDGLPEDERDELQ
                     QRLAALRDDVPPVRFAELEKLMREEFGGPLGRVFRDFDERAFAAASIGQVHRATTIDG
                     DDVAVKIQYPGVAEAVDTDLRNAMMLLPLVKRLAPGLDAKALAAEMRERIGEELDYEL
                     EAQNQRRIGRLLRGHPFVRVPRVDTSLSTRRVLVSEYVEGERFEAVRRADEVERDRYG
                     EIVFRLYFGLLYRDRIALGDPHPGNYLLCPDGRVCFLDFGLLRGVDAARVAAERAIAL
                     AVRYRDAEALKAALREGGYLPASRADAVDADFALSLMRMAIKWYAVPGERRFSPVDRR
                     DRRDRDRERPDPEERAAMRMQMNQFTLPPEAILIRRMHGIVAVVLGQLRAGADWGAIA
                     AEYLHGEAPATPLGQAEAEFLARRGRTASAALGPAN"
     gene            4828..5883
                     /locus_tag="VF752_05115"
     CDS             4828..5883
                     /locus_tag="VF752_05115"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8854960.1"
                     /translation="MAKNGVFKRSRAEAAAAAAVAGVAAAGGKLALDRRSKRIDEERC
                     FRLRVDEAVPDGIRRIARGQIERAHDELESASGRKIGPAVHEARKSLKRLRAAVRLSR
                     DAIGDERYRQENTAFRDAGRRLSGARDAKVLIETLDSVEGAAGDELTAGMSAGLRAQL
                     ETEHEAALESLRERGATAAVLGDLEKARALVATWTLATDGFDSIEPGLRRIYRRGRNA
                     MQAVAEEQSTENLHEWRKRVKDLWHATQIVRPASPGRMKKMSRRLHDLSDLLGDDHDL
                     AVLRAYVNRHPQCLEDRDQQAALVAVIERRRGLLQGKALSLGSTLYEQSPKEFTRSLS
                     RRWRKRVGKPRRLAAAV"
     gene            complement(5897..6385)
                     /locus_tag="VF752_05120"
     CDS             complement(5897..6385)
                     /locus_tag="VF752_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX8854961.1"
                     /translation="MRGVEIERKFLVPELPQGLERSQATRIEQGYIAIAGDGTEVRVR
                     RRDGETLLTVKGGSGRSRTEEEIEIDAGRFTRLWPLTEGRRLEKTRYVIPAGSGLSIE
                     LDVYSGALTGLSVAEVEFASEEAADAFEPPAWFGAEVTDDARFKNQRLASEGAPADVR
                     RA"
     gene            complement(6440..7378)
                     /gene="tatC"
                     /locus_tag="VF752_05125"
     CDS             complement(6440..7378)
                     /gene="tatC"
                     /locus_tag="VF752_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8854962.1"
                     /translation="MEAFPLRAVAHDERLTITEHLTELRARLLLAAAVLAVLFAGCLW
                     QSHTLLHVLNVPLAHLRTSPAAQPSGRQLPQALARSADAFSRLAHSSSLGPQDQRAAL
                     SAAHSLAAASQSFQRPETRAPVTLGPGEPFSTSVTVAFAFALVLGLPFLLTQAWAFVA
                     PAIAPAERRAVRPLLILAPALFAAGVAFGYFLVLPPAVKFLQGFNHGAFDVLVRARDY
                     YRFELMTMLVLGAIFEIPVLLLALGRAGVLSSATLRRHRRYAIVGLAVLGSLLPGADP
                     VTMLLETLPLFALYEASIVLLRFNERRSRRLKSLSG"
     gene            complement(7446..7655)
                     /gene="tatA"
                     /locus_tag="VF752_05130"
     CDS             complement(7446..7655)
                     /gene="tatA"
                     /locus_tag="VF752_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8854963.1"
                     /translation="MIENILSPTHLLLILAVALIVLGPKRLPEAGRGLGSAIRGFKDS
                     LSTAESSDEQPAIPTPSEPDHAPRT"
     gene            complement(7697..8782)
                     /locus_tag="VF752_05135"
     CDS             complement(7697..8782)
                     /locus_tag="VF752_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8854964.1"
                     /translation="MENAEQSWRVTDRRSFLLKGAAVGAGAIGASRLLADPSPASADA
                     GLTDGDVAILQFLAAAELLEADLWEQYNELAGIQDREVPGGRGNRAYTKALSVLDEDM
                     NQYIHDNTDDERSHVRFINAYLEAHGAGSINLDKFRTLPSSKARGARQIGRLTNLMEL
                     TVDTSWWTRYRSDSKNPDLGDKLPQAIPDLAVGRHPAIPRSDADLTPRKHLQAIANTA
                     GFHFAAIEQGGSSLYASLAQRVTSPEVLRILLSIGPTETMHFQTWQDKAGNAPPLTDP
                     KTGLKFPDLNGHGELLQTNLIMPEPTVFLDRKFPTVSIIRPTETEGAAVGAAKALTAS
                     GLFKGQSQEFFSAVTALAQAADAAQRQ"
     gene            9416..9784
                     /locus_tag="VF752_05140"
     CDS             9416..9784
                     /locus_tag="VF752_05140"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX8854965.1"
                     /translation="MLAGLRLARAVGNSRAMSSWRNDEVFPGAAVHDGGQLQRSGSVG
                     TYFHPVGTCKMGTDPAAVVDLELRVHGVECLRIVDASVMPSLPAANTNATVRAIAERA
                     VAIITGDEHVPAEPVIGAAA"
     gene            complement(9831..10616)
                     /locus_tag="VF752_05145"
     CDS             complement(9831..10616)
                     /locus_tag="VF752_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8854966.1"
                     /translation="MHAENTSQKIAVTGATGRVGSHLVEILEQRGHDVVPIARSKGVD
                     VVTGEGLDEALVGVETIIDTATGPSPDQDEATAFFTASARNLQRAGAAAGAKRIVVVS
                     IIGIDKFQGGYNAAKVAQEQTLLEGPLPVRIVRAAQFHEFVEPLVGWTIQDGVAYVPE
                     MRTQLVAARVVAEALADAAEEPEIENGRITEVAGPQEERLAEAAAALFASRGDSIEIR
                     ESRGGPLADPGDPDAAAYAEGAALPNPGAKLAGPSFEEWLKEA"
     gene            10901..>12402
                     /locus_tag="VF752_05150"
     CDS             10901..>12402
                     /locus_tag="VF752_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854967.1"
                     /translation="MNASKRTLSAFFVVAGLLVTAVGLSAWAQTPQHSTDAPAGARPP
                     STNPPWYPSLEAFEHYNSGRSHVFSMARFGGSLHGRNRVDLVRSRRGAYPSGYNMSYL
                     NAKAAFIQGGSYGDVKNSIGPFVAKVDPKTLKPVWYTQLVNTVQTGEWDYPGAMAIMN
                     DGFIYVVSGYRIFKVDPANGKVVKTLKLPTLVHMRNNYPNTPPTYNTTLTDDAVNTSY
                     NGINALPDGTIVVKSLYRQAGCTLNGPSALLNCPEARNVPASNLISVNPETMRIIDNI
                     TLPTFAGARPTITRYRGVDYVYLLENTSNAVRYSVRHGIFTLDTKWTPAAVPYPNQTT
                     GGSLIIMNNWVLGATNSVPAAGPLTVFAINQSDAKKVFYLQPYVNDPIPPLLYRAFAT
                     AADGAQAISWADMSLEADPQNGLFYGVETLARKVAAFKLTASGIKTVWKKTQTTTEWA
                     TLIGPKAHRVWVGTDIPGVEIPGQNKTERIVFRDAATGRELARSARVLQMT"
BASE COUNT         2003 a         4225 c         4215 g         1959 t
ORIGIN      
        1 gccgagcccg acggcctgct gcttcccttc ctgctgcacc cgacgccgga gctgttggag
       61 cgccaatcgc gacttcgcag cactcacact tcgctgatct ccgagatcct gaacctgctg
      121 tcgggacgca gtccggcggc ccgccccgag gacgccgagc cgctccagga agcgctctct
      181 gagaccgagc ttcgggtgct gcgctacctg ccgaccaacc tccggggccc cgaaatcgcg
      241 tcggagctgt tcgtgtcgct gaacacgatc agggcccacc tgcgcaacat ctacgccaag
      301 ctcggcgtgc acagccgtgc ggacgcggtc aagcgggcgc gcgagctcgg cctgctggcg
      361 ccctcgtcgc tcaagcgctg acccaggaca ccgtcagctt cgtgcgggcc ggaactgcat
      421 caaccatgcg aggcgcactc gcatcgcgag ggctcaccct ggcaggcatg agcgaaaggc
      481 cgctaccgaa ctcaaggagc agggtcatgt cccacggtga agagcagcgg cagatggagg
      541 caatcgtcat cgaggcaagc gggccgccgg cgcagtcgtt gacttaccgg tccgagccgg
      601 tgccggagcc gggtcccgga gaggtgcttg tcgaggtgca cgcagcggca gtgaacccgc
      661 tcgacatcgc caacattctg ggtctgctcg gcacgccgct gccgaagatc cccggcgtcg
      721 acttcgccgg tgtcgtcgtc tccgacggcg acagccttgg cgaggaggtg tggggcagcg
      781 ggccagcgct gggcatggcg ttggacggga atcgagcggg cacgcacgcg cgctgcgtcg
      841 ccattcccgc cacgtggctg tcgcgcaagc ctgagcgcct gacgatggcc gcggccgccg
      901 cggtcggccg atcgcattgg gcggcctggg agacgctgat caacatgatg gagatcacgc
      961 ccggcgagac gatcctgatc accggcgggg caggcttggt cggccaggcc gccaccgcaa
     1021 tcgctcgctg gcgcggcgca gaggtgatca tcgccgaccg gcgcaagccc gacgacgtca
     1081 tgcagttcat cgacaccacc tcgagcgaca tccgcgaggc ggtgctcgaa ctcaccgatg
     1141 ggcgcggtgc cgacctggtg ctggacacgg tgggcggcga gctgttcgtg cctgctctcc
     1201 ggagccttcg gtacgacggg cggatggtcg ggcttcacgg tggcaccgag ccggtcacgt
     1261 tcgacctgaa tgagatctac aaccggcagc tgcacgtcac gggactcgcc accgtgttct
     1321 acgacggggc gcacgtcgcg cgcatcttcg accaattccg ggcgctgttc gatcacggcc
     1381 tcctcgaccc cccggccgtc aagacgtggc cgctcgagaa ggcggccgag gcataccaga
     1441 cggtgctgga cgggtcggcc ggaattaagc aggtgctgct gccggccggc cgcgtggcgt
     1501 agcgaggttc gcgatgagct cagaccccgc gcttcttccc gactcgatgc gggccccggg
     1561 gctcacgcag ttcgctgcgc caacggcggc actcgccacc gtgcgactgc ggctgccgac
     1621 ggccgcacag ggtgagccgc ttgggccatc gtcctccgtc gatccattca ccccacagga
     1681 actcgccgct tggcgggggc ttctggagct tcacgcacaa gtaatccgcc agctggacgt
     1741 ccagctgcac gccctgcacg gcctgacgtt gtcgcaatgc gaggtgctga tcttgctcga
     1801 cgacgctccc ggcgagcgaa tgcgcatgac agagctcgcc gcgggcgtgc tgctcagtcg
     1861 tagcgcgtgc acgcgcctcg ttgatcgtct cgagacgctc ggatacgtca ccaggcgcgc
     1921 agcgacggat gatcggcgcg gcctatacgc caagctcacc gacaccggcc ggcatgtgat
     1981 cacgcccgca cgagcgaccc accgagaggg tgtgcgcgcg gcctttcttg accgactccg
     2041 cacgaccgac caagtcgctc tcgacgacat ttgggcgcgc gtccgcgggg cgcttccgag
     2101 ctccgaccca acgggacgca cagtctctca gacagcacgg aagccgtgat cagctgcaac
     2161 cctcaaagcg accgggcgaa gggggcgaaa gccggagcac tccttcgctc acgacaccgc
     2221 cccgtccgat tgcggagcgc gctggtgcaa tcggacacaa cgagccacgg cacaacactc
     2281 gcgatacccg tgcagagcaa agcgagtctc ggccggcgcg tcgccgcgca cctccctttc
     2341 gccaggcgag ggtgtgacgg cgggtccact cgaacggtgc gggcgacccg ccgcccatcc
     2401 ggattacgga gcgatttcaa gcccggtgct tgcagagatc atgggcgacg gccgcccatg
     2461 cacggtgtcg atgatctcgg cgctgtcgat cccctgtagg tacacgctcg tgatgccgag
     2521 attcgcgtga ccgagctgcc gctggatgac cacgagcgga acgccttcgt gcgccatctc
     2581 gaccgcgtgt gcgtgccgga gttgatgcgg cgcgaaccgc cgccggactc cagccgccgc
     2641 ggccgcgtgg tggagctgct ttcgtgcagc tgacgcctcc cagtgacgtc cggccgtggg
     2701 gccgtggatc acgcacagca gcgccccgat cggcagctcg cgccgtttct ccaaccacgg
     2761 ctcgagctgg gcccacgccc atctatccat gcccacctca cggcgcttgc cacccttacc
     2821 gtggcggacc aggatcgcgc cgcgagccgc gtcgaggtcg ctctcctgga gagcgagcgc
     2881 ctcgctgatc cgcagccccg cgcgccacag aagcatgatc agggcgcgga gtcgctttcc
     2941 ctcggcgcga tcgccgacgg cgcgcatcac agcgacgatc tcctcgacag ttggcgggtc
     3001 ggctggatag tgctcgccct tgttgcgcgg cggccggccg gggtgatagc ccggcatcgt
     3061 cgccggcgac cggcgctggc cgacgacatc cagcaaaacg gattccatgg atcctccctc
     3121 cggtgggtag gttgacaatc cgctgctatc tgaaccgagc tcaacaccgc ggtcggggtg
     3181 atccaagttc gcggatgttc caccgggcgc cgccacctgg tgtccgcgct ttgcgaggct
     3241 gctgcggccg cggccgggct gcttctgtcg aggtgaatgc aggagcccgc gcgatgccta
     3301 gttggcgggc ccgagggcgg cgcttgccgt ccgaccgcgc cgtgcgagga actcggcttc
     3361 agcctggccc agcggcgtgg cgggcgcctc gccgtgcagg tactccgcgg cgatcgcgcc
     3421 ccagtctgcg ccagcgcgca gctgtcccag gaccacggcg acgatgccgt gcatccgccg
     3481 gatcaggatc gcctccggcg gcagagtgaa ttggttcatc tgcatcctca tcgcggcccg
     3541 ttcctccggg tccggccgtt cacgatcgcg gtcgcggcgg tcgcggcggt cgacggggga
     3601 gaagcggcgc tctcccggga ccgcgtacca cttgatcgcc attcgcatca agctcagcgc
     3661 gaaatcggcg tccacggcat cggcgcggct cgccggcaga tagccgccct cgcgcagcgc
     3721 cgccttgagg gcctccgcgt cccgataccg aaccgcgaga gcgatcgccc gctcggcggc
     3781 cacgcgcgcg gcgtcgacgc cgcgcagcag accgaagtcg aggaagcaca cccgaccgtc
     3841 gggacagagc aggtagttgc cgggatgagg gtccccgagc gcgatccggt cgcgatagag
     3901 caacccgaag tagagccgaa agacgatctc gccgtagcgg tcgcgctcca cttcatcggc
     3961 ccggcggacc gcttcgaagc gctccccctc gacgtactcg gacacgagca cgcgccgggt
     4021 cgacaggctc gtgtcgactc gcggcacgcg gacgaacgga tgtcctcgca gcagccgccc
     4081 gatgcggcgc tggttctggg cctcgagctc gtagtcgagc tcctcgccga tccgctcgcg
     4141 catctccgcg gcgagcgcct tcgcgtccag gcccggggcg agccgcttca cgagaggcag
     4201 cagcatcatc gcgttgcgca gatccgtgtc gaccgcctcc gccaccccgg ggtactggat
     4261 cttcacggcc acgtcgtcgc cgtcgatcgt cgtcgcgcga tggacctgac cgatcgaggc
     4321 cgctgcgaaa gcacgctcgt cgaagtcgcg gaacacgcgc ccgagcggac cgccgaactc
     4381 ctcgcgcatc agcttctcga gctccgcgaa ccgaacaggc gggacgtcgt cgcgcagcgc
     4441 cgcaagccgc tgttgcagct cgtcccgctc gtcctcggga aggccgtcga actcgaccat
     4501 cgagatcatc tgaccgacct tcatcgccgc gccacgcatc tgcccgagct gatcgacgag
     4561 ctgctggacc agcgccgcgg tgcgctcttt ctgggcggcc gtcgcgcgct caggcgtgcg
     4621 aatccggttc gcggttcgca tcccggccca gcgcaggccc tgccccgtca cgagcccgcc
     4681 gacacgcgcc gtccgggcga gtcgcccttt gggcagcttt cccgcctctc ggagcgaatc
     4741 ttcgtcctgc gcgcccattg accgagcgta gcgggggctc cgcttcggaa cggttacgaa
     4801 cgcggcggtt ccggacaacg gaacggtatg gccaagaacg gagtcttcaa acgctcgcgc
     4861 gccgaggctg ccgccgcggc ggcggtcgcc ggcgtggcgg cagcgggcgg caagctggct
     4921 ctcgaccggc gctccaagcg catcgacgag gagcgctgtt tccgcctccg cgtcgacgag
     4981 gccgtgcccg acggtatccg ccgcatcgcc cgggggcaga tcgagagagc gcacgacgaa
     5041 ctggagagcg cgtcagggcg gaagatcggc cccgccgttc atgaggctcg gaagagcctc
     5101 aagcggctgc gcgccgctgt gcggttgtcg cgcgacgcga tcggcgacga gagatacagg
     5161 caggagaaca cggccttccg ggacgccggc cgccggctgt ccggagcacg cgacgcgaag
     5221 gtgctgatcg agacgctcga ctccgtggag ggggccgccg gcgacgagct gaccgcgggc
     5281 atgagcgcgg gactgcgggc gcagctcgag acggagcacg aggcggcgct cgagtccctt
     5341 cgcgaacgcg gcgccaccgc ggccgtcctg ggcgacctcg agaaagcccg tgcgcttgtg
     5401 gccacgtgga ccctcgccac ggacggcttt gactccatcg agcctggcct gcggcggatc
     5461 taccgccgcg gccgcaacgc gatgcaggcc gtcgcggagg agcagagcac cgagaacctg
     5521 cacgagtggc gcaagcgcgt gaaggatctc tggcacgcaa cccagatagt caggccggcc
     5581 tcgccgggcc gcatgaagaa gatgtcgcgg cgcctccacg acctgtccga cctactcggt
     5641 gacgatcacg acctggccgt cctgcgcgcc tacgtgaacc ggcatcctca gtgccttgag
     5701 gaccgggacc agcaggcggc gcttgtggcg gtgatcgagc gccggcgcgg gcttttgcag
     5761 ggcaaggcgc tgagcctcgg aagcaccctc tacgagcagt cgccgaagga gttcacgcgc
     5821 tcgctatcgc ggcgctggcg aaagcgtgtg ggtaagcctc gccggctggc cgcagcggtc
     5881 tgacaaatga gcaacctcac gcgcggcgca cgtccgccgg tgccccctcg gacgccagcc
     5941 gctggttctt gaagcgagcg tcgtcggtca cctcagcccc gaaccacgcc ggcggctcga
     6001 acgcatccgc ggcctcctcg gaggcgaact cgacctcggc cacggacaaa cccgtgaggg
     6061 cgcccgaata gacgtcgagc tcgatgctca ggccggagcc ggcggggatc acgtagcggg
     6121 tcttctcgag ccggcggccc tcggtgagcg gccacaggcg agtgaaacgc ccggcatcga
     6181 tctcgatctc ctcctccgtg cgactgcggc cgctgccgcc cttcaccgtc agcagcgttt
     6241 cgccgtcgcg gcgccggacc cgaacctcgg tgccgtcgcc cgcgatcgcg atgtaaccct
     6301 gttcgatccg cgtcgcctgt gaccgctcga gaccctgcgg gagctccggg acgagaaact
     6361 tgcgctcgat ctccacgccc cgcatcgaac cacagagccg agggcgccgt tgcgggagca
     6421 gtgtcggctt gaaacgccgt tagccagaca gtgatttgag gcggcggctc ctgcgctcgt
     6481 tgaatctcaa caagacgatg ctggcctcgt agagggcaaa gagcggcagc gtttccagca
     6541 gcatggtgac ggggtcggcc cctgggagga ggctgcccag caccgcgagc ccgacgatgg
     6601 cgtagcgtcg atggcggcgc agggtggccg agctgaggac gcccgcgcga ccgagggcga
     6661 gcaggaggac ggggatctcg aagatcgcgc cgaggacgag catcgtcatt aactcgaagc
     6721 ggtagtagtc gcgcgcccgg acaagcacgt cgaaggcgcc gtgattgaac ccctgcagga
     6781 acttgacggc cggcgggagg acgaggaagt acccgaacgc cacgccggcg gcgaagaggg
     6841 caggcgcgag gatcagaagc ggccgcaccg cacgtcgctc ggcgggagcg atcgccggcg
     6901 cgacgaacgc ccacgcctga gtgagcagaa agggcagccc gagcactagc gcgaaggcga
     6961 aggcaacggt gaccgaggtg gagaacggct cgcctggacc gagcgtcact ggcgcccgcg
     7021 tctccgggcg ctggaacgac tggctggcag cggcgaggga gtgggctgcg ctcagcgctg
     7081 cccgctggtc ctgcgggccg agcgatgacg agtgggcgag tcggctgaag gcgtcggcgc
     7141 tgcgagccag cgcttggggc aactgccggc cggaaggttg cgccgccggg ctcgttcgaa
     7201 ggtgagcgag cgggacgttg aggacgtgca ggagcgtgtg gctctgccag agacaaccgg
     7261 cgaacagcac cgcgagcacc gcagcggcga gcaggagccg cgctcggagc tcagtgaggt
     7321 gctcggtgat tgttaggcgc tcgtcgtgcg cgacggcgcg tagcgggaag gcttccatac
     7381 gcttggggcc tcgagcgtca ggggcggcga caaatgccgc cgccccctaa gcgctcagca
     7441 cttggtcaag tgcgcggggc gtggtccggc tcgctcggtg ttgggattgc cggctgctcg
     7501 tcggaagact cggcggtgga gagcgagtcc ttgaagccgc gaatcgccga gccgaggccg
     7561 cgcccggctt ctggcagccg cttcgggccg agcacgatca gtgcgacggc caggattagc
     7621 agcaggtgcg tggggctgag gatgttctcg atcatggtgt gtcgtccttg tcgtttcgag
     7681 tcgctggtgt cgctcgtcac tgacgttgag cggcgtcggc agcctgtgcg agcgccgtga
     7741 cggccgagaa gaactcttgt gactgcccct tgaacagtcc gctggcggtc agggccttgg
     7801 cggcgcccac ggcggcgccc tcggtctcgg tgggtcggat gatcgagacg gtcgggaact
     7861 tgcgatcgag gaacaccgtg ggctcgggca tgatcaagtt cgtttgcaac agctcgccgt
     7921 ggccgttcag gtcggggaac ttgagtcccg tcttggggtc ggtcaggggc ggagcgttgc
     7981 ccgccttgtc ctgccaggtc tggaagtgca tcgtctcggt ggggccgatg ctgagcagga
     8041 tgcgcaacac ctccgggctc gtcactcgct gcgcgagcga ggcgtagagg cttgagccgc
     8101 cttgctcgat cgcggcgaag tgaaagcccg ccgtgttggc gatcgcttgc aagtgcttgc
     8161 gcggcgtgag gtccgcgtcg ctgcgcggaa tcgccgggtg acgacccacc gccaggtccg
     8221 ggatggcttg cggcagcttg tcgccgaggt ccgggttctt cgagtcgctg cggtaccgcg
     8281 tccaccagct ggtgtcgacg gtcagctcca tgaggttcgt cagccggccg atctgacgcg
     8341 cgccgcgtgc cttgctgctg ggcagcgtgc ggaacttgtc caggttgatc gaacctgcgc
     8401 cgtgcgcctc cagatacgcg ttgatgaacc tgacgtggct ccgctcgtca tcggtgttgt
     8461 cgtggatgta ctggttcatg tcctcgtcga gcaccgagag agctttcgta tacgcccggt
     8521 ttccgcgtcc gccggggact tcacggtcct ggatgccagc gagctcgttg tactgctccc
     8581 acaggtcggc ctcgaggagc tcagccgcag cgaggaactg aagaatcgcc acgtcgccgt
     8641 cggtgaggcc cgcatccgcg gaggccggcg agggatccgc cagcaggcgg ctggcaccga
     8701 ttgctccggc tcctaccgcc gcgcccttga gcaggaagga gcgacggtct gtcacacgcc
     8761 aagactgctc ggcgttttcc atgatttctc cttgtgagtt gcggaagcgt cggactgcac
     8821 gtagggcagg gagccgacct cccgttcgaa atacggtcgc gccatggtcc gcgccggtaa
     8881 ggcgtttgtc gtcggctcgc ggccgacact ttcggctgat gcctgcgcag tactgtcgcc
     8941 atgggcgtac agcagatacg gcactgcaaa gtgacgcccg ccggcggacg acagagaggc
     9001 tgcagagctt gagttcttac gacttcatag ttgtgtgtgg aggcacggcg ggatccgtcc
     9061 tggcgacgcg cctatccgaa aaccccgagg tgcgggtgct cctgctcgag gcgggccccg
     9121 ctcaagggcc cgagacgatg tcgatccccg gagcctggcc gttgctgatc ggcagcgagg
     9181 tcgactgggg ctacaggacc ctgcctcagc ccggattggg gggtggcgcc atcccctatc
     9241 cgcgcggcag ggtcctcggc ggctccagcg ccatcaatgc gatgttccac ctgcggggcc
     9301 acagatccgc catcgacgcc tgggcgaccg cgggcgcgac cggctggggc tacgacgacc
     9361 tactcccgta cttccgtcgc agcgagcgta cggaggggct cgataagtgg acgatatgct
     9421 cgccggactt aggcttgcgc gcgcagtcgg caactcgaga gcgatgtcgt cttggcgcaa
     9481 tgacgaggtg tttcctggcg ccgcagttca cgatggcggt cagctgcagc gaagcgggag
     9541 cgttggcacc tacttccacc ccgttggcac ctgcaagatg gggacggatc cagccgccgt
     9601 ggtcgacctc gaactgcggg tacatggggt cgaatgcctc cgcattgtcg acgcgtcggt
     9661 aatgccgtcc ttgccggcgg cgaacaccaa cgccaccgtc cgcgccatcg ccgaacgcgc
     9721 cgtggccatc atcacgggtg acgagcatgt gccggcggag cccgtcatcg gagcagcagc
     9781 gtgagcgccg ccgaccagtc gcctccggat ccggtggtga ggaactaccg ctatgcctcc
     9841 ttcagccact cttcgaagct ggggccggcg agcttcgccc ccgggttggg cagcgcggcg
     9901 ccctccgcat acgccgcggc gtccgggtcg ccggggtccg ccagcgggcc gccacggctc
     9961 tcgcggatct cgatcgagtc acctcggctg gcgaagagcg ccgctgccgc ctcggcgaga
    10021 cgctcctcct gcgggccggc gacctcggtg atcctgccgt tctcgatctc cggctcctcg
    10081 gccgcgtcgg cgagagcttc ggcgacgacg cgggcggcca cgagctgcgt acgcatctcg
    10141 ggcacgtagg cgacaccatc ctggatcgtc cacccgacga gcggctcgac gaactcgtgg
    10201 aactgggccg cgcgcacgat ccggaccggg agcgggcctt cgagcagtgt ctgctcctgc
    10261 gccaccttcg ccgcgttgta gccgccctgg aacttgtcga tgccgatgat cgagacgacg
    10321 acgatccgct tcgccccggc ggcggcgccg gctcgctgca ggttccgcgc cgaggcggtg
    10381 aagaatgccg tcgcctcgtc ctggtcgggc gagggcccgg ttgccgtgtc gatgatggtt
    10441 tcgaccccga cgagcgcctc atcgagaccc tcgccggtga ccacgtcgac accctttgag
    10501 cgcgcgatcg ggactacgtc gtgtccgcgc tgctcgagga tctcgacgag gtgagatccg
    10561 acccgtccgg tggctcccgt gaccgcaatc ttctggctcg tgttttcagc gtgcatcgat
    10621 cttcctttct cggggcgagg gcccgacatc ggcagatttc atccgtcact cgtgtgacac
    10681 ccggcggcgc gaagatgtga cacccggagt cgctcgctac accaagagca gggccaggcg
    10741 aaccagccag atgaagcccc gctcctcgcc gagaagcggg ctggcgacgt ttggcggtcg
    10801 ggtgtacgat ccgatcgcga tttgactcga tcggctgatc cgctcggctg gtccgtggtt
    10861 gtgtgccaat ggctgcttga gccgggtcgg cgggcacgca atgaacgctt ccaagcgcac
    10921 attgtcggct ttcttcgttg tagccgggct gctcgtgacg gccgtagggc tcagcgcttg
    10981 ggctcaaact ccccaacatt cgacggatgc tccggccggc gctcggcctc ctagtaccaa
    11041 cccgccgtgg tatccgtcgc tcgaggcatt cgagcattac aactccggcc ggtcgcacgt
    11101 gttctcaatg gcgaggttcg gcggatcgct gcacgggcgg aaccgggtgg atctggtccg
    11161 gtcccgcagg ggcgcgtacc cctccggcta caacatgtct tacctcaacg ccaaggccgc
    11221 cttcatccag ggcggcagct acggcgacgt caagaactcg atcggcccgt tcgtcgcgaa
    11281 ggtcgacccg aagaccctga agccggtctg gtacacgcaa ctcgtgaaca cggtgcagac
    11341 cggcgaatgg gactaccccg gagcgatggc gatcatgaac gacggcttca tctacgtcgt
    11401 gtccggctac cggatcttca aggttgatcc ggccaacggg aaagtcgtga agaccctcaa
    11461 gctgccgacg ctggtccaca tgcgcaacaa ctaccccaac acgccgccca cctataacac
    11521 gaccctgacc gacgacgcgg tcaacacctc gtacaacggc atcaacgcgc tcccggacgg
    11581 gacgatcgtc gtgaagtcgc tgtaccgaca ggctgggtgc acgctgaacg ggccgtcggc
    11641 gctcctcaac tgccccgaag cgcgaaacgt tccggcgtcg aacctgatct cggtcaaccc
    11701 ggagaccatg cggatcatcg acaacatcac tctgcccaca ttcgcgggcg cccggcccac
    11761 catcactcgc taccgcggcg ttgactacgt gtacctgttg gagaacacct ccaatgcggt
    11821 gcgttactcg gtgcgccacg ggatcttcac cctggacacc aagtggactc ccgccgccgt
    11881 tccctatccc aaccagacga ccggcggctc gttgatcatc atgaataact gggttctcgg
    11941 cgcgacgaac tcagttcctg ccgcggggcc gctgacggtg ttcgcaatca accagagtga
    12001 tgccaagaag gtcttctacc tgcagcctta tgtcaacgac ccgatcccgc cgctcttgta
    12061 ccgcgccttc gcgaccgccg ccgacggggc acaggcgatc agctgggccg acatgtcatt
    12121 ggaggccgat ccccagaacg gcctcttcta cggggtggag acgctggccc gcaaagtcgc
    12181 agcattcaag ctcactgctt caggcatcaa gaccgtgtgg aagaagaccc agaccaccac
    12241 cgagtgggcc acgctcatcg gcccgaaggc tcaccgcgtc tgggtcggca ccgacattcc
    12301 cggagtcgag atccccggcc agaacaagac cgagaggatc gtcttccgcg acgcggccac
    12361 gggtcgagag ctcgcacgct cggcgagagt cctccagatg ac
//
LOCUS       DASVXS010000089         6769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1296355, whole genome shotgun sequence.
ACCESSION   DASVXS010000089 DASVXS010000000
VERSION     DASVXS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296355"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VF752_05155"
     CDS             <1..1557
                     /locus_tag="VF752_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX8854968.1"
                     /translation="YLLNAREAIAIAKQTPQYQRTLRAHPGLRVFAYTNNGPGRWQVS
                     GFAGGKELVQVLIDDRNGRVTEAWTGPQVAWKMARGYPGAFGRSVNSPWIWIPLCVLF
                     VAPFVDPRRPFRLLHLDLLVLLSFGISHFFFNRGEISTSVPLVYPVLAYLLVRMLFAG
                     FRPRAGRGPLIPFAPTWLLVAGVVLLMGFRIGLNMTDSNVIDVGYAGVIGADRIEHGR
                     DVYGTFPRDNEHGDTYGPANYLLYVPFELAEPWDGSWSKLGSANGAAIGFDLVTAAGL
                     FLLGMRMRAGPEGRRLGAGLTWAWVSFPYALFVLETNANDSLVAASLVLALLALSRPV
                     LRGIALGVGAAAKFVPLVLAPLFAVGAGSDRGRRQALIFGATMVVVIAALVLPFVPDG
                     GLHEFWDRTIAYQAGRMTPISIWGQHNSLGWLQEECKGAAIVLALLVAFVPRRRDLRQ
                     VAALGAAVLLALQISGGYWFYLYVVWFAPFVFVALFAAHPAGLRRAPASAEPEEEHAR
                     AGREAAPALT"
     gene            1572..2810
                     /locus_tag="VF752_05160"
     CDS             1572..2810
                     /locus_tag="VF752_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854969.1"
                     /translation="MRGPLLLLGAGAMVSVFVYPWNARYISDVWLYWGYGGDIVHGHL
                     PYSAFNFEYPPLAALVIGLPAIPAASHALTYEGLFGALMLVAAIGCLLLCARLAELSG
                     GNAKRAAYAFASLPLATGALAREHLDLVPVLLLLAGLAVIARGRSTTGGFGLLGLGTM
                     TKAFPLLAAPPAVAWLWARDRRESALRGAAVLVAIVVVLAGAVYALSPSGARYAWNYQ
                     VKRPVQVESVPAIVLQAIDAAGGPEVRRFPTHNGDGMTSSASSAVSAGSTALLAGALV
                     AATLLAARGARDGPLARRRLVLAALTAVAAFPALGKVLSPQFLIWTWPLFALAFAWGE
                     LALASLVLTATVLTFVEFPFQYFAVTRGETGAVLLVAARDAVLIAAVGLAFARLAALS
                     ASREKDLLDRPRTPVFARVD"
     gene            complement(2745..3527)
                     /locus_tag="VF752_05165"
     CDS             complement(2745..3527)
                     /locus_tag="VF752_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX8854970.1"
                     /translation="MKLVFVGDVVGAPGRRALEDLLPGMRERYEPDFVVVNGENAAGG
                     LGITEEIARHFLDDLGVDAITLGNHAYRHREVYRFLDSDQRIVRPANYARANPGRGST
                     VVENGSGRLGVINLSGAVFIEGSMRSPFSEADALLSDMNGRADYLLVDFHAEATSEKV
                     AMGWHLDGRVTACLGTHTHVATADARVLPGGTAYITDVGMTGPRGGVIGVKREQALER
                     FRTLMPVRFDTSEEDPWLNAVLVDAGEDGRARSIEQILLPAG"
     gene            3566..4312
                     /locus_tag="VF752_05170"
     CDS             3566..4312
                     /locus_tag="VF752_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8854971.1"
                     /translation="MQQPGAAPPSAADSRESAVIEATGLVKRFGEREALRGVSLSAER
                     GELVAIIGPNGAGKTTLLSILARIQKPNEGGVRWPRGDVGWVPQQAAVYNKLTVSENL
                     RLFARLEKVADVEATVERMLAQTGLDDRRDDPVSELSGGNRQRVNIAIGLLSEPEVLL
                     LDEPSAALDPRQRERLWEFILDLAARGTTVVYSTHNVQEADRHANRLVVLADGELLFT
                     GSPRELEGAVGSTGLDFEQAFVAFLRQHGH"
     gene            4314..5654
                     /locus_tag="VF752_05175"
     CDS             4314..5654
                     /locus_tag="VF752_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8854972.1"
                     /translation="MRWLLLKDLQILRRSPFLVALLVLYPIVIALLIGFAVSRGPEKP
                     RVAILNQVPESAQTLSLAGENVDIDKYTQQLYKAIEPVPVQSRKEALDKVKNGDALAA
                     LIVPPDLTQKLQTGLEPGQVEVFYNAEDPVKAQFVRDTIKSQVQDANTALTKKFTQIA
                     LSYLKLISTGGRISLLGQTFDVLGLQNAERIVTEAQRELPKDSAARAQLDQVTRFARL
                     ARENLNLSNGVLASVGTPIRVNSHVIKGGSTPLSSFAVAIALTFSLMFVTVLLAAGVL
                     ALEREENAFLRLVRGLVSRTGLLIEKVSLSAICALGVSLVMLGALGLFVDLAWHRFPL
                     WLAAIVLGALGFAAMGVAIGGITREVRAASLLAVMISLPIAFLALVPSGAVSAGLYDV
                     IRVISAIFPFKPTLEALDAALNKSGSIGGPLLHLAILTVAFTAIARLALRRFAS"
     gene            5679..6653
                     /gene="hemB"
                     /locus_tag="VF752_05180"
     CDS             5679..6653
                     /gene="hemB"
                     /locus_tag="VF752_05180"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8854973.1"
                     /translation="MAFPATRMRRLRKTGVLRDLVRETELSASDLVYPMFIEHGIDSR
                     TPIEAMPGIERLSISNAVEEAGQVHTLGIPAVLLFGIPASKDEVGSGAYDDEGVIQLA
                     TRAIKEAHPDLLVITDVCLCEYTSHGHCGVLTPDGQVDNDPTVELLARTAVSQANAGA
                     DAVAPSDMMDGRVGALRSHLDAEGHADLPVISYSAKFASAFYGPFREAAGSTPAFGDR
                     RSYQMDSANAEEAVRESLLDVEEGADMLIVKPALPYLDVIRRVKEATRLPVAAYNVSG
                     EYAMIKAAAAAGYLDERAAVLEALTGIRRAGADIVITYHAKDVAGWLK"
BASE COUNT          905 a         2347 c         2371 g         1146 t
ORIGIN      
        1 tacctgctga acgcgcggga ggcgatcgcg atcgcaaagc agaccccgca gtaccagcgc
       61 acgctgcgcg cgcatcccgg cttgcgcgtg tttgcctaca cgaacaacgg cccgggccgc
      121 tggcaggtga gcggcttcgc cgggggcaag gagctcgtgc aggttctgat cgacgatcgc
      181 aacgggcgcg tcaccgaggc gtggaccggc ccgcaggtcg cgtggaagat ggctcgcggc
      241 tatcccggcg cgttcgggcg cagcgtcaac tcgccctgga tatggattcc gctctgcgtg
      301 ctgttcgtcg cgccgtttgt ggatccacga agacccttcc ggctactgca cctcgatctg
      361 ctcgtgctgc tctcgttcgg catctcgcac ttcttcttca accgcggcga gatctccacg
      421 tcggtgccgc tcgtatatcc ggtgctcgcg tatctgctgg tgcgcatgct gttcgcgggc
      481 ttccgcccgc gcgccgggcg cggcccgctg atccccttcg cgccaacctg gctgctcgtc
      541 gccggcgtgg tgctcctcat gggcttccgc atcggcctga acatgaccga ctcgaacgtg
      601 atcgacgtgg gctatgcggg cgtgatcggc gccgaccgca tcgagcacgg gcgcgacgtc
      661 tacggcacct tcccgcgcga caacgagcac ggggacacct atggaccggc gaactacctg
      721 ctctacgtgc cgttcgagct cgccgagccg tgggacggct cgtggtcgaa gctcggatcc
      781 gccaacggcg cggcgattgg cttcgacctc gttaccgcgg cggggctgtt cctgctcggg
      841 atgcgcatgc gcgcgggtcc cgagggccgg cggctgggcg cgggtctcac ctgggcgtgg
      901 gtgagcttcc cgtacgcgct cttcgtgctc gagacgaacg ccaacgactc gctcgtggcg
      961 gcctcactcg tgctggcgct gcttgcgctg tcgcggccgg tgctgcgcgg catagctctc
     1021 ggcgttggcg ccgcggcgaa gttcgtgccg ctcgtgcttg cgccgctctt cgccgtgggc
     1081 gccggcagcg accggggacg caggcaggcg ctcatcttcg gcgcgacgat ggtggtggtg
     1141 attgccgcgc tcgtgcttcc gttcgtgccg gacggcggcc tgcacgagtt ctgggaccgg
     1201 acgatcgcgt accaggccgg acgcatgacg ccgatctcga tctgggggca gcacaactcg
     1261 cttggctggc tgcaggagga gtgcaagggc gccgcgatcg tgctcgcgct tctcgtcgcg
     1321 ttcgtgccgc gacgccgtga cctccgccag gtggcggcgc tcggtgccgc ggtgctgctc
     1381 gcgctccaga tctcgggtgg ctactggttt tacctgtacg tggtctggtt cgccccgttc
     1441 gtgttcgtgg cgctgttcgc cgcgcacccc gcgggccttc ggcgggcgcc cgcgtcggcc
     1501 gagccggagg aggagcacgc gagggccggt cgcgaggcgg cgcccgctct cacatgacgc
     1561 gcctttgcac gatgcgcggg cctttgctgc tgctcggagc cggcgcgatg gtgagcgtct
     1621 tcgtctatcc ctggaacgcg cgctacatct cggacgtctg gctgtattgg ggctacggcg
     1681 gcgacatcgt gcacggccat ctgccgtaca gcgcgttcaa cttcgagtac ccgccgctcg
     1741 ccgcgctcgt gatcggtctg cccgcgattc cggccgcctc ccacgcgctg acctacgaag
     1801 gcctcttcgg ggcgctcatg cttgtcgcgg cgatcggctg cctgctgctc tgcgcgaggc
     1861 tcgccgagct gagcggcgga aacgcgaagc gcgccgccta tgccttcgcg tcgctaccgc
     1921 tcgcgaccgg cgcgctcgcg cgcgagcacc tcgacctggt gccggtgctg ctcctgctcg
     1981 ccgggctcgc cgtgatcgcg cgcggccgca gcacgacggg cggcttcggg ctgctcgggc
     2041 tcggcacgat gaccaaggcg tttccgctgc tcgccgcacc gcccgccgtc gcctggctct
     2101 gggcccggga caggcgcgaa agcgctctgc gtggcgccgc cgtactggtc gcgatcgtgg
     2161 tcgtgctggc cggggcggtc tacgcgctgt cgccgtctgg cgcgcgctac gcgtggaact
     2221 accaggtgaa gcgaccggtg caggtcgaga gcgtgccggc catcgtgctg caggcgatcg
     2281 acgccgcggg cgggcctgag gtccggcgct tccccacgca caacggcgac ggcatgacga
     2341 gctcggcgtc gagcgccgtc agcgcgggct ccaccgcgct cctcgcagga gcgctggtcg
     2401 cggccacgct tctcgcggcg cgtggcgccc gcgacgggcc gctcgcgcgt cgccggctgg
     2461 tgcttgcggc cctcacggcg gtggctgcgt ttccggctct cggcaaggtg ctgtcgccgc
     2521 agttcctgat ctggacgtgg ccgctgttcg cgctcgcgtt cgcctggggt gagctcgcgc
     2581 tcgcgagcct ggtgctgacg gcgaccgtcc tgacgttcgt cgagttcccg ttccagtact
     2641 tcgcggtgac ccgaggcgag acgggcgccg tgctcttggt ggcagcgcgc gacgccgtcc
     2701 tgattgcggc ggtgggcctt gccttcgctc ggttggcggc gctttcagcc agcagggaga
     2761 aggatctgct cgatcgaccg cgcacgcccg tcttcgcccg cgtcgactag gaccgcgttg
     2821 agccacgggt cctcttcgga ggtgtcgaag cgcacgggca tcagcgtgcg gaagcgctcg
     2881 agcgcctgct cccgcttcac gccgatcacg ccgcccctcg gcccggtcat gccgacgtcg
     2941 gtgatgtagg cggttccgcc tggaagtacg cgtgcgtcgg cggtagccac gtgtgtgtgg
     3001 gtgccgagac acgcggtcac gcggccgtcc agatgccagc ccatcgccac cttctcgctc
     3061 gtggcctcgg cgtggaagtc gacgagcaga tagtcggcgc gtccgttcat gtccgagagc
     3121 agcgcgtccg cctcgctgaa gggcgaacgc atcgagccct cgatgaagac ggcgcccgac
     3181 aggttgatca cgccgagccg cccgctcccg ttctcgacga ccgtgctgcc gcggccagga
     3241 ttggcgcggg cgtagttggc ggggcgcacg atccgctggt cggagtcgag gaagcggtac
     3301 acctcgcgat ggcggtaggc gtgattgccc agtgtgatgg cgtcgacgcc gaggtcgtcg
     3361 aggaagtggc gggcgatctc ctcggtgatg cccagcccgc cggccgcgtt ctcgccgttc
     3421 acgacgacga agtccggctc gtatcgctcg cgcatgccgg ggagaaggtc ctccagggcg
     3481 cgccgccccg gtgcgccgac cacgtcgccc acgaagacca gcttcatccg gcccccgatt
     3541 cttccattcc ttctatcttc gacccgtgca gcagcccgga gccgccccgc cgtccgccgc
     3601 ggattcgcgc gagtcggcgg tgatcgaagc caccggcctc gtgaagcgct tcggcgagcg
     3661 cgaggcgctt cgcggcgtct cgctgagtgc ggagcgcggg gaactcgttg cgatcatcgg
     3721 accgaatggc gccgggaaga cgacgctgtt gtcgatcctc gcgcgcattc agaagccgaa
     3781 cgagggcggg gtgcgctggc cgcggggcga tgtgggttgg gtcccgcagc aggccgccgt
     3841 ctacaacaag ctcaccgtgt cagagaacct gcggctgttc gcgcggctcg agaaggtggc
     3901 cgacgtggag gcgacggtcg agcgcatgct cgctcagacg ggcctcgatg accgccgcga
     3961 cgacccggtg agcgagctct cgggaggcaa tcgccagcgc gtgaacatcg cgatcgggct
     4021 gctgtcggag ccggaggtgc tgctgctcga cgagccgagc gccgcgctcg acccccgtca
     4081 gcgcgagcgg ctctgggagt tcatcctcga cctcgcggcg cgagggacga cggtcgtcta
     4141 ctccacccac aacgtgcagg aggcggaccg ccacgcgaac cggctggtgg tgcttgcgga
     4201 cggcgagctg ctgttcaccg gctcaccgcg cgagctggag ggcgccgtcg gttcgaccgg
     4261 cctcgacttc gagcaggcgt tcgtcgcgtt cctccgtcag cacggccact gacgtgcgct
     4321 ggctgcttct gaaggacctt caaatcctgc ggcgctcgcc gttcctcgtc gcgctgctcg
     4381 tgctctaccc gatcgtgatc gcgctgctga tcggcttcgc ggtttcgcgc ggtcccgaga
     4441 agccccgtgt ggcgatcctc aaccaggtcc ccgagtcggc gcagacactg tcgctcgccg
     4501 gagagaacgt ggacatcgac aagtacacgc agcagctcta caaggcgatc gagccggtgc
     4561 cggtgcagtc ccgcaaggag gcactcgaca aggttaagaa cggcgacgcg ttggccgcgc
     4621 tgatcgtgcc gccggacctc acccagaagc tccagaccgg gctcgagccc ggccaggtcg
     4681 aggttttcta caacgctgag gatccggtga aggcgcagtt cgtccgcgac acgatcaagt
     4741 cgcaggtgca ggacgcgaac accgccctca ccaaaaagtt cactcagatc gcgctcagct
     4801 atctgaagct catatcgacc ggcggacgca tctcgctgct cggccagacc ttcgacgtgc
     4861 tgggtctgca gaacgccgag cggatcgtga ccgaggcgca gcgggagctg ccgaaggact
     4921 cggcggcgcg cgcgcagctc gatcaggtga cgcgcttcgc ccgcctggcg agggagaacc
     4981 tgaacctctc gaacggcgtg ctcgcctccg tcggcacgcc gatccgtgtg aactcccacg
     5041 tgatcaaggg cggctccacg ccgctcagct cgttcgctgt ggcgatcgcg ctcacattct
     5101 cgctgatgtt cgtgaccgtg ctgctcgcgg ccggcgtgct cgcgctcgag cgtgaggaga
     5161 acgcgttcct gcggctggtg cgcggactcg tctcgcggac tggattgctg atcgagaaag
     5221 tgagcctgtc ggcgatctgc gcgctgggcg tgtcgctcgt gatgctcggc gcgctcgggc
     5281 tgttcgtgga cctcgcctgg caccgcttcc cgctctggct cgccgcgatc gtgctcggcg
     5341 cgctcggctt cgccgccatg ggtgtcgcga tcggcgggat cacgcgcgag gtgcgtgcgg
     5401 cctcgctgct cgccgtgatg atctcgctgc cgatcgcctt cctggcgctg gtcccgtccg
     5461 gcgcggtgtc ggcgggcctc tacgacgtga tccgcgtgat ctcggcgatc ttcccgttca
     5521 agcccacgtt ggaggcgctc gacgccgcgc tgaacaagtc gggctcgatc ggcggcccgc
     5581 tgctccacct ggcgatcctg acggtggcgt tcacggcaat agcgcggctg gcgctgcgcc
     5641 gcttcgccag ctaggtctgg agccctagac tgactttcat ggccttcccc gcgacccgca
     5701 tgcgccggct gcgcaagacc ggcgtcctgc gcgacctggt ccgtgagacc gagctgagcg
     5761 caagcgacct cgtctacccg atgttcatcg agcacgggat cgacagccgc acgccgatcg
     5821 aggcgatgcc cggcatcgag cggctctcga tctcgaacgc ggtcgaggag gccgggcagg
     5881 tgcacacgct aggcatcccg gcggtgctcc tgttcggcat tccggcgagt aaggacgagg
     5941 taggcagcgg cgcttacgac gacgagggcg tgatccagct ggccacgcgc gcgatcaagg
     6001 aggctcaccc agacctgctc gtgatcaccg acgtgtgcct ctgcgagtac acctcgcacg
     6061 gccactgcgg cgtgctcacg ccggacggcc aggtcgacaa cgacccgacg gtggagctgc
     6121 tcgcgcgcac ggcggtgtcg caggccaacg ccggcgccga cgcggttgcg ccgagcgaca
     6181 tgatggacgg gcgcgtcggt gctctgcgct cgcatctcga cgccgagggc cacgccgatc
     6241 tgccggtcat ctcctacagc gcgaagttcg cgagcgcctt ctacggtccg ttccgggagg
     6301 ccgccggctc cacgccggcc ttcggcgacc gccggagcta ccagatggac tcggccaacg
     6361 ccgaggaggc ggtccgcgag tcactcctcg acgtggagga aggcgccgac atgctgatcg
     6421 tgaagcccgc gctgccctac ctcgacgtga tccgccgcgt gaaggaggcc acccgcctgc
     6481 cggtcgcggc ctacaacgtg agcggcgagt acgcgatgat caaggcggcc gctgccgccg
     6541 ggtacctcga cgagcgcgcg gccgttctcg aggccctcac cgggatccgc cgcgccgggg
     6601 ccgacatcgt gatcacctac cacgccaagg acgtagccgg atggctgaag tgagcagccg
     6661 cgccgcgggc gcgatcccga aggtgcgcag ccgcagggac ggcgccgccg tgccgctcga
     6721 cgagctcgac aagaagctgc tcaacctgat gcagggcaag ttcccgatc
//
LOCUS       DASVXS010000090         6274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_719071, whole genome shotgun sequence.
ACCESSION   DASVXS010000090 DASVXS010000000
VERSION     DASVXS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719071"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VF752_05185"
     CDS             <1..1173
                     /locus_tag="VF752_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854974.1"
                     /translation="RRVEQRLRAARAPGEVDAASRSFDVVRAGFEAREPRRGASRART
                     RIGAAIVLAVLAAALVLSPAGAKVAHFVGDAFDGPGVRHAKPALVSLPAPGRLLVDSR
                     SGTWVVQQDGSKRLLGPFHDPAWSPHGLFVAAVRGRELLAVDPKGNVRWSLARDGLVE
                     LPSWSPDGFRIAYRSGSALRVVTGDGTGDRPLARTAEAVRPAWRPGAGHLVAFLARQQ
                     VRQVDADSGRVQWNTTVFNSKGKLEWTPDGRRLVVGGPLGLRVLDGHGHVVRSLDAPP
                     GWLQRDVAISPRGDSVAIVRQEARTGRSEVLRVSMNRRGSPQRLFAGAGRIQQVQWSP
                     DGRWLLLAWRDADQWLFVRSSAARGLRAYSGIAAAFNPRHPRAGLFPAAAGWCCSR"
     gene            complement(1170..1928)
                     /locus_tag="VF752_05190"
     CDS             complement(1170..1928)
                     /locus_tag="VF752_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8854975.1"
                     /translation="MSAASITLVRHATLIIEIGGRRLLLDPMLDTPGARPPIDNSPRP
                     RANPLVPLPMPAPRVVRGIDAAAITHLHADHFDGTARELLPPGTTVLCQPEDAPRLVN
                     LGDWELIPVGAELEWEDVRVIRTGGVHGFGELGENLGPVSGFVFVSGRRRIYVAGDTV
                     WCPPVAEALERYQPTAIVLNAGAARFLEGDTITMDAGDVATVARAAPDAFVMAVHMEA
                     INHCLLTREELREALWQENLTVAIPADGETIALA"
     gene            complement(1925..2734)
                     /locus_tag="VF752_05195"
     CDS             complement(1925..2734)
                     /locus_tag="VF752_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8854976.1"
                     /translation="MPEAPPAGSGERLPAAIERALPLLERSARERATPAATRHGYLDL
                     IGEQPPESTGRTQDLMLSRGMPRVYERWWRPAFGRIAKGVFGPGMEEERRIARLLLGL
                     SPGDGVLDVACGTGNFSRDFGRSVGPEGLVVGIDVSEPMLTRAVEETRNAGLDNVAYV
                     RGPAEALPFRDESFDAVCCFAAFHLFANPERALDHMTAVLTPGGRIAIFTSARGGSSA
                     LRTVESLVEAQSGMRMFERDEVVQMLEQRGYVEISQRLTGMTQFVGGRLAG"
     gene            complement(2738..3004)
                     /locus_tag="VF752_05200"
     CDS             complement(2738..3004)
                     /locus_tag="VF752_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854977.1"
                     /translation="MSEFERPDRLTREPTVEDVRELMGASTPHFALQIRNRIRNLIVG
                     LPDEHPARRLGEQEIARLDRLAYEGEHRGHPAQPDEPPLPSIEV"
     gene            complement(3030..4199)
                     /locus_tag="VF752_05205"
     CDS             complement(3030..4199)
                     /locus_tag="VF752_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX8854978.1"
                     /translation="MTRILPIAVLCSAVALAGCSLNDSKGGATPDVSAKANDSSAAQQ
                     LGLPLIATKNTTRVSGADPATDAAGVVSAVFPSTNEVNKPRAIALVDKSNWAGAVAAG
                     VLMSRPLEVPMLVSDGGKLPSVTKDTLGRLKPGGSPLAKNAQVIRVGDSAANPGGLRT
                     GIIHGKDPYTLAAAVDRFWSVVRGKPSPSVVIASGERADFAMAAGAWAARSGDAVLFT
                     QRNTVPAATLKAIRQHSRPSIVILGPDFIISKDVEKQLGKLGKVSRIDGPTPVQTAIA
                     LARTQRGGVGWGITVPGQNFTIANSTRPMDAAAAAPLAGRGVFAPLLLTDQATSLPNS
                     LESYLLDVQPGFENNPNAGVYNHVWILGDKAAVSLPAQGRIDQITELVPVQVRKP"
     gene            4333..5601
                     /locus_tag="VF752_05210"
     CDS             4333..5601
                     /locus_tag="VF752_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8854979.1"
                     /translation="MDFRLTDDQLEWQQYCRTFARDVIRPVAAKHDREQSTPWEVLKE
                     ARKRKLHGMEYLEKVASDPGGLYGVIYAEELHWGCAGIALAISASSLAAAGIAASGTP
                     EQIGQWLPECYGTGDEIKLAAYAVTEAGAGSDVKALRTTAKLEGDEWVLNGTKVFISN
                     GGIADVNVVVATVDPELGHRGQASFIVPKDTPGLSAGKKEDKIGIRASHTGEVVLEDC
                     RIPTDYLLGGMDKLNAKLERARSGKSTGRASGALATFEITRPLVGASALGIAQAAYEW
                     TLEYLDDKEEFGEPLMESQRIQQTLADVATEIEAARMLVWRAAWMGRNGIPMTAGQGS
                     MSKLKAGDVTMWATTTLMDLVGDYAQSTECPLEKWFRDAKIYQIFEGTAQVQRLVVSR
                     MQRAVYNERRADVLEQAPGTPTGDAVGATA"
     gene            5598..5840
                     /locus_tag="VF752_05215"
     CDS             5598..5840
                     /locus_tag="VF752_05215"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX8854980.1"
                     /translation="MSEAVSQRNKLRIEVDRELCFGFGDCVDSAPSVFELDAENKSVV
                     VDPDGADRDTILLAAQDCPVDAIAIIDDETGEQIFP"
BASE COUNT          902 a         2131 c         2271 g          970 t
ORIGIN      
        1 cggcgggtgg agcagcggct gcgcgcggca cgcgcaccgg gcgaggtcga tgcggcgagc
       61 cgctcgttcg atgtcgtgcg cgccggtttc gaggcgcgcg agccgcgccg tggcgcatcg
      121 cgtgcgagaa cgcggatcgg cgccgcgatc gtgctcgccg tgctggccgc ggcgctcgtg
      181 ctttcaccgg cgggtgccaa ggtcgcgcac ttcgtcggcg acgcgttcga cggcccgggt
      241 gtgcggcacg cgaagccggc gcttgtgtcg ctccccgcgc cgggcaggct gctcgtggac
      301 tcgcgctccg gcacgtgggt cgttcagcag gacggatcaa agcggctgct cggtccattc
      361 cacgacccgg cctggtctcc gcacggactc ttcgttgccg cggtgcgcgg tcgggaattg
      421 ctcgcggtcg atccgaaagg caacgtccgc tggtcgctcg cgcgcgacgg tctcgtcgag
      481 ctcccttcct ggtcgcccga cggcttccga attgcgtacc gctccggctc ggcgctgcgc
      541 gtggtcacgg gcgacggcac gggcgaccgg cctttggcga ggacagcaga ggccgtgagg
      601 cccgcttggc ggccgggcgc gggtcacctg gtcgccttcc tcgcgcgcca gcaggtgcgg
      661 caggtcgacg ccgacagcgg gcgcgtgcag tggaacacaa ccgtcttcaa cagcaaaggg
      721 aagctcgaat ggacgcccga cggtcgccgg cttgtcgtag gcgggccgct cggcctgcgc
      781 gtgctcgacg ggcacgggca cgtggtgcgt tcgctcgatg cgccgcctgg ttggcttcag
      841 cgcgacgtgg ctatctcccc gcggggtgac agtgtcgcga tcgtgcggca ggaggcccgc
      901 accgggcgga gcgaggtgtt gcgggtgagc atgaaccggc gcggctcgcc ccagcggctg
      961 ttcgcgggcg ccgggcggat ccagcaagtg cagtggtcgc ccgacgggcg ctggctcttg
     1021 ctggcgtggc gtgacgccga ccagtggttg ttcgtgcgct cgagcgccgc gcgcgggctc
     1081 cgcgcctact cgggcatcgc cgcggcgttc aatccgcgcc atccgcgtgc cggcctgttc
     1141 ccggccgccg ccgggtggtg ctgcagccgc taggcaaggg cgatcgtctc gccgtcagcg
     1201 ggtatcgcga ccgtcaggtt ctcctgccag agcgcctcac gcagctcttc gcgagtgagc
     1261 aggcagtggt tgatcgcctc catgtgcacc gccatgacga aagcatcggg tgccgcgcgg
     1321 gcgaccgtcg caacgtctcc ggcgtccatc gtgatcgtgt cgccctcgag gaagcgcgcc
     1381 gcaccggcgt tcagcacgat cgcggtgggt tggtagcgct cgagggcctc tgccacaggc
     1441 gggcaccaga cggtgtcgcc ggcaacgtag atccgcctgc ggccgctcac gaacacgaag
     1501 cccgaaaccg ggccgaggtt ctcgccgagc tcaccgaagc cgtgcacgcc gccggtgcgg
     1561 atcacgcgga cgtcctccca ctcgagctcc gcgccaaccg ggatcagctc ccagtcgcca
     1621 aggttcacca ggcgcggggc gtcctcgggt tggcagagca ccgtggtccc gggcgggaga
     1681 agctcgcgcg ccgtgccgtc gaagtggtcg gcgtgcagat gcgtgatcgc cgccgcgtcg
     1741 attccgcgca ccacgcgcgg cgcgggcatc ggaagcggca cgagcgggtt cgctcgcggg
     1801 cgcggcgagt tgtcgatcgg cggccgcgcg ccgggcgtgt cgagcatcgg gtcgagcagc
     1861 agtcgcctgc cgccgatctc gatgatcaag gttgcgtggc gcacgagcgt gatgctcgcc
     1921 gcgctcatcc tgccagccgc cctcccacga actgggtcat acccgtgagc cgctggctga
     1981 tctccacata gccgcgctgc tcgagcattt ggaccacctc gtcgcgctcg aacatgcgca
     2041 tgccgctctg ggcctcgacg agcgactcga cggtgcggag ggcgctcgat ccgccccgcg
     2101 ccgaggtgaa gatcgcgatg cgaccgccgg gcgtgagcac ggcggtcata tggtcaagcg
     2161 cgcgctcggg attggcgaag aggtggaacg ccgcgaagca gcagaccgcg tcgaagcttt
     2221 cgtctcggaa ggggagcgcc tcggccggcc cgcgcacgta cgcgacgttg tcgagcccgg
     2281 cgttacgcgt ctcctccacg gcgcgtgtga gcatcggctc cgagacgtcg atgccgacca
     2341 cgaggccctc aggccccacc gagcggccga agtcgcgcga gaagttgccg gtgccgcagg
     2401 cgacgtcgag cacgccgtcg ccgggactga ggccgaggag cagccgcgcg atgcgccgct
     2461 cctcctccat gcccgggccg aacacgccct tggcgatgcg gccgaacgcg ggacgccacc
     2521 atcgctcgta cacgcgcggc atcccgcgcg acagcatcag gtcctgtgtg cggccggtcg
     2581 actccggcgg ctgctcgccg atcaggtcga ggtagccgtg gcgtgtcgcc gcgggcgtcg
     2641 cgcgctcgcg tgcggagcgc tcaagcaggg gaagcgcgcg ctcgatcgca gccgggagac
     2701 gctcgccaga tccagccggc ggcgcctccg gcaccggcta tacctcgatg ctcgggagcg
     2761 gcggctcgtc cggctgggcg ggatgaccgc ggtgctcgcc ctcgtaggcg aggcggtcga
     2821 gccgggcgat ctcctgctcg ccgagccggc gcgccgggtg ctcgtccggc aggccgacga
     2881 tcagattccg gatccggttt cggatctgca gggcgaaatg cggcgtcgac gcgcccatca
     2941 actcgcgcac gtcctcgacg gtcggctctc gcgtgaggcg gtcgggtcgt tcgaactcgc
     3001 tcatcggacc gggagggtac atgccacgct catggctttc ggacctgcac cggcacgagc
     3061 tctgtgatct ggtcgatgcg cccctgcgcc ggaagcgaca ccgcggcctt gtcgccgagg
     3121 atccagacgt ggttgtagac gccggcgttc gggttgttct cgaagcccgg ctgcacgtcg
     3181 agcaggtagc tctcgagcga gttgggaaga gaggtggcct gatcggtgag gagcagaggc
     3241 gcgaacacgc cgcgaccggc gagcggcgcc gcggcggccg catccatcgg tcgtgtgctg
     3301 ttcgcgatcg tgaagttctg gcccgggacg gtgatgcccc agccgacgcc gccgcgctgc
     3361 gtgcgggcta gggcgatggc cgtctgaacc ggcgtgggac cgtcgatccg gctcaccttg
     3421 ccgagcttgc cgagctgctt ctcgacgtcc ttgctgatga tgaagtcggg cccgagaatc
     3481 acgatgctgg gacgcgagtg ctggcggatc gccttcagcg tggcggcggg cacggtgttg
     3541 cgctgcgtga agagcaccgc gtccccagag cgcgccgccc acgcgcccgc ggccatagcg
     3601 aagtcggcgc gctcgcccga cgcgatcacc accgacggcg acggcttgcc gcgcacaacc
     3661 gaccagaagc ggtccactgc tgcggccagc gtgtacgggt ccttgccgtg gatgatcccg
     3721 gtgcgcaggc cgccggggtt ggcggcgctg tcgcccacgc ggatcacctg tgcgttcttg
     3781 gcgagtgggg agcctccggg cttcaagcgg ccgagcgtgt ccttcgtgac cgacggcagc
     3841 ttgccgccgt cgctcaccag catcggcacc tcgagcgggc gcgacatcag cacgccggcc
     3901 gccacggcgc cggcccagtt ggacttgtcg acgagcgcga tcgcgcgcgg cttgttcacc
     3961 tcgttcgtgc ttggaaacac tgcgctgacg acgcccgcgg cgtcggtcgc ggggtcggcg
     4021 cccgacacgc gcgtggtgtt cttcgtggcg atgagcggca ggccgagctg ctgcgcggcg
     4081 gaactgtcgt tcgccttcgc gctcacgtcg ggggttgcgc cgcccttgct gtcgttgagg
     4141 ctgcagcccg ccaaggcaac cgcggagcag aggacggcga tggggaggat gcgagtcaac
     4201 gagaagcgca cgataacgag cggtccgggc ctccgcttca ggcggtattc tcagattggt
     4261 tgactggtca gtcaaccggc atcgaggcgt cgccctgcgc gacgggctct tggattagga
     4321 gaggacttca acgtggattt ccgcctgacc gacgaccagc tcgagtggca gcagtactgc
     4381 cggacgttcg cgcgcgacgt gattcgcccg gtggctgcca agcatgaccg cgagcagtcg
     4441 accccatggg aggtgctcaa ggaggcgcgc aagcggaagc tgcacgggat ggagtacctc
     4501 gagaaggtcg cgagcgaccc gggcggcctc tacggcgtga tctacgcgga ggagctgcat
     4561 tggggctgcg cgggcatcgc gctcgccatc tcggcgtcgt cgctcgcggc cgcgggcatc
     4621 gcggcttccg gcacgccaga gcagatcggc cagtggctcc ccgagtgtta tggcacgggc
     4681 gacgagatca agctggccgc gtacgccgtc actgaggcag gtgccggctc ggacgtcaag
     4741 gcgctccgca cgacggcgaa gctcgaaggc gacgagtggg tcctgaacgg caccaaggtc
     4801 ttcatctcga acggcgggat cgccgacgtg aacgtggtcg tcgcgaccgt cgaccccgag
     4861 ctcggccatc gcggccaggc cagcttcatc gtgccgaagg acacgcccgg gctgtcggcg
     4921 ggcaagaagg aggacaagat cgggatccgc gcctcgcaca ccggcgaggt cgtcctcgag
     4981 gactgccgca tcccgaccga ctacctgctc ggcggcatgg acaagctgaa cgcgaagctc
     5041 gagcgtgccc gctcgggcaa gtcgaccggc cgcgcctcgg gcgccctcgc gacgttcgag
     5101 atcacgcgcc cgctcgtggg cgcctcggcg ctcggcatcg cccaggccgc atacgaatgg
     5161 acgctcgagt acctcgatga caaggaggag ttcggcgagc cgctcatgga gtcgcagcgg
     5221 attcagcaga cgctggccga cgtcgcgacc gagatcgagg cggcgcgcat gctcgtgtgg
     5281 cgcgcggcgt ggatggggcg caacggcatc ccgatgaccg cgggccaggg ctcgatgtcg
     5341 aagcttaagg cgggcgacgt gacgatgtgg gccacgacca cgctgatgga cctcgtgggc
     5401 gactacgcgc agtccaccga gtgcccgctc gagaagtggt tccgcgacgc caagatctac
     5461 cagatcttcg agggcacggc ccaggtgcag cggctcgttg tctcgcgcat gcagcgggcg
     5521 gtctacaacg agcgccgcgc cgatgtgctc gagcaggcgc cgggcacgcc gacgggcgac
     5581 gcggtcgggg ccaccgcgtg agcgaagccg tttcgcagcg caacaagctg cgcatcgagg
     5641 tcgatcgcga gctctgcttc ggcttcggcg actgcgtgga ctcggcgccg tccgtgttcg
     5701 agctggacgc cgagaacaag tcggtggtcg tcgacccgga cggggctgac cgcgacacga
     5761 ttctgctcgc cgcgcaggac tgcccggtcg atgcgatagc gatcatcgac gacgagacgg
     5821 gcgagcagat cttcccgtag cgctgttgct gctgtacggc ctgtggagtc ggggccgttc
     5881 cccttcgggc tcccttcgcg cttcgcgctc actcttgacg cccttcgggg aaggcccctc
     5941 ctcccaggcc tacgccagcg ccgcgccagt ccactcgctt tgagaagcag gatccggcgc
     6001 cgcccaaggg ttcaaaagtt ccaactcgcc gcccgcgccc gaacgacatg gacgttatgc
     6061 accgtatagg tgcatttcgt acatctcgct tccgcgaatc gctccagtta tgcacccctc
     6121 gcgctctccc cttagtgctt aactgagccg ccgcggcgct cgagggcgac caccggcgtc
     6181 gcggccgggg gctcgagcgg gaagaagttc catctcgatc ccgttcccgc gacgccatgg
     6241 aagtttgtcc cgctatgcgg gaaggacttc catg
//
LOCUS       DASVXS010000091        11994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_448639, whole genome shotgun sequence.
ACCESSION   DASVXS010000091 DASVXS010000000
VERSION     DASVXS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448639"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1676
                     /locus_tag="VF752_05220"
     CDS             81..1676
                     /locus_tag="VF752_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854981.1"
                     /translation="MPVARLLSVHAVLAGLALASLGMVAPAARAAGGDIGYRDQSYAP
                     LGGSPTSSKPESKLWFNNGWWASMYNAGAGEHRIYKLNSALGVWSDSGVAIDKRASSR
                     ADALWDAGSKKLYVASHVYTTSGTATTSGSAGRLYRYSYDAAGDRYALDPGFPVTVNA
                     AKTETLVIDKDSTGTLWATWTQGSRVYVNHTLGGNDAAWGTPYVVPGPGTTLTSDDIS
                     SVIHFGGNKIGVMWSNQADHNFYFSVHADGAGDASWSTSAVPSAAAGSDDHINLKADS
                     AGRVYAAVKTAANNKSPDSTPEILLLVRSAGGAWGSATFGSAADSETRPIVLLDEQHG
                     LIHMFATCPQPPSTSGQAGGDICEKTTSMSSPSFAAGIGTAVIRDASSPKMNDATSTK
                     QEVDGGTGIVVMANNASTDSYWHMQESLGAPPSAPAPAPVPKPARTSPRLRITSKRWR
                     GLRLYVRGTLSRKATGRVKVSYLKGRPGKRIRARKSATIKRGHFAVSLRLPRRARGHK
                     AILWASYGGDRSYLAQAVRARVR"
     gene            1682..2932
                     /locus_tag="VF752_05225"
     CDS             1682..2932
                     /locus_tag="VF752_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854982.1"
                     /translation="MRAAARVLALPVALAAFAFATLGTVARPATAAGGDIGYRDQSYV
                     PLGGSPTGSKPESKLWFNNGWWASMFSPAAGDHHIYKLDRASRAWADTGVAIDPRNST
                     RADALWDAGSQKLYVASHVYTTSGKATTSGSSGRLYRYSYDAAGDRYSLDPGFPVTVN
                     SAKTETLVIDKDSTGTLWATWTQGSRVYVNHTLGGGDAAWGRPYIVPGSGTTLTSDDI
                     SSVIHFGGNKIGVMWSNQVDHHFYFSVHDDGSDDSASSWSTSVVPAGASSDDHINLKT
                     DSAGRVYAAVKTSETSKSRPLVLLLVRAAGGPWSSATFGTVSDSHTRPIVLLDEQHGL
                     IDMFATCPQPPKTSGQAGGDICEKTTSMSSPSFSPGIGTPLIREAGSPKMNDATSTKQ
                     NVDSATGIVVMANNASTDTYWHMQ"
     gene            2934..4517
                     /locus_tag="VF752_05230"
     CDS             2934..4517
                     /locus_tag="VF752_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8854983.1"
                     /translation="MAAAPSTLHQPSVATRAASSRHPATGSDAARRQIRGSSLLLAGR
                     MLSLAVNFATQVLIVRYLSKADFGAFAYGLSIVTLGESLAVLGLDKAISRFLPIYDEQ
                     RAYGKLFGTLLMVAGTVLSVGLAFVLLAFGLHGFAGGELGASSQALAVILILICLSPI
                     QALDDVLMGTFAVFSKPRAIFFRKYVLAPGLRLTAILLIVLAQSGVRELALGYVIAGG
                     LGVMLYVAMLVQTLRADGLLAHLHVPGLRFPVREVFGFALPLITVDLLFVVMNTTNVW
                     MLERWGSAADVADYRVVQPAARLNLLVMTSFTLLFTPLAARLFARGDRQGVRELYWRT
                     AAWIAVFSFPVFALTFVSAHELTIALFGGRYAGSAPILALLAAGYYFNAALGFNGLTL
                     RVYGLVRYTVVISVAAAVANVAINLLLIPRYGAIGAGVGTCATLLIHNVLKQAGLLKG
                     TGIGVFNREYMRVYGAIVVTIAALVALHVLLAPGAFVAIVLVAVASALLLWITRETLR
                     VGDTFPELLRVPLLRRLVR"
     gene            complement(4524..6143)
                     /locus_tag="VF752_05235"
     CDS             complement(4524..6143)
                     /locus_tag="VF752_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854984.1"
                     /translation="MLAHARASTFVAGANREREGAGAAWTFLLPSLELGLVLVVGAPS
                     ARTLRTLSRLASEVVVCCTRESDRRRVRRRAVEVGAPNVRVLRPERAYEAAREVDLAV
                     VGGARAATTLGSKEVRQAVTAAQAAFLDLAGREAWALQAGVCAPCALRMIPARGEVHA
                     AVAEGDISALAYLEQHRTRPASRPLQWHGRRSRRGVLAVPGADEARLPVPTYVRTIAG
                     AAGVGLDEHRAALWAPSEYPSRKAVLFLFRGDEPVPELVVKLTRDGAYNSRLENEWRA
                     LQWLQDEDLHGLGALPHPAFFGHHGGLALLGASAVAGSPFRERTTAQADCPLARAALE
                     WLLELGAATAHPAAADRDVAAALEDLLGRFEALYALTAVERSRLHRHVDALAQAARPM
                     PIVLQHGDPGTWNLLVTPGGRPAFLDWEAAEHEGMPLWDVFYFVRSFAVTVARAAGVR
                     SSLRGLRAQLLADTPVNRLLVAAVERQCARIALDRELVEPLFVTCWMHRALKEATRLR
                     PARLDRGHYVNLLRISLDEADSPGLRRLFAA"
     gene            complement(6328..6903)
                     /locus_tag="VF752_05240"
     CDS             complement(6328..6903)
                     /locus_tag="VF752_05240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8854985.1"
                     /translation="MDVCLAAGALLLLAPLLGVIAACVWLEEPRAPVIFRQRRCGYGG
                     RSFQLFKFRTMVRDADALKDALRARSLVPWPDFRVVNDPRVTRVGRFLRRSSFDELPQ
                     LVNVLRGEMSLVGPRPTSFDASTYALWQTGRLEYRPGITGPWQVYGRGCLDFEERCRL
                     EIGFFRRRSITRELWLLIQTLGCVMRRTGVA"
     gene            complement(6943..7770)
                     /locus_tag="VF752_05245"
     CDS             complement(6943..7770)
                     /locus_tag="VF752_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEX8854986.1"
                     /translation="MRNAAAPQLPVLAHGTTDAAERQLRIFALGRLRVETADGSISDD
                     WLDQRPGQLLRYLVCARAASADAIAEAICPNAPATAPGNVRYFVHTLRTRLEPRRPKH
                     GDSSFLVSRCGGYSLDPQRVWIDVDDFERDAAAGLSAFAHGDRCAAAELLERAIALYR
                     DDFLADDPYAVWALTERERLRALACEALRALAALRTSDPVEASSYLERLAELEPFDSE
                     VQRDLICTWLRLGRKTRALRHYQSFRVRLLREFGEQPDFELVDLVNQRRFVGAAASR"
     gene            complement(7763..9193)
                     /locus_tag="VF752_05250"
     CDS             complement(7763..9193)
                     /locus_tag="VF752_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8854987.1"
                     /translation="MALFTRFSRVMFGSLAATFVFALSLAIPASALGTGAGQDTQAGT
                     PPMASASWHGRPIRDPLQHSGAVVATSADAHAVRFGAGYDTPGGSERVRSIQAMLRTL
                     GYQPGPVDGLFGPRTRAAIQWFQIKHGFARTGAADAATLAYLRFRSTAAKGAAKAQAP
                     NAPAPLRPVVSQPVTKQLARTPADLRKTGKPARGLLVALLLIVAVVAIGAGCAATVAA
                     LRRRGRRQGRSSARKAQRRGPSVPARPAPVPEPPQPRAVGYVRHADAGGHAAAIEQAC
                     SRRGWTLVAVARDRGKADRRPALAQALGQISSGAASRLVVARLDELARSRYDLASLLD
                     WFAARRAGLVAVDVGLDTTTRDGAAAARALLPTTRIERWRGADRVRNRHQPSVRGTRR
                     TSRPAVADRPELVQRIRAMRETGMTLQAIADTLNKEGVPTARGGAQWRPSSIQSTLGR
                     RTHTSRSLARWARARIRNGIRKASHA"
     gene            9824..10003
                     /locus_tag="VF752_05255"
     CDS             9824..10003
                     /locus_tag="VF752_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854988.1"
                     /translation="MKTGPPLYTSAPRALFRALPAADKGGDRRQAAILAAAALFAVVA
                     ASGSLLRMTSRMRLR"
     gene            10000..11121
                     /locus_tag="VF752_05260"
     CDS             10000..11121
                     /locus_tag="VF752_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854989.1"
                     /translation="MKRLLVVGAAVGVVGWTMPATASAYDIKVSCTGSSGIPVACGQW
                     HNFPVTLRLVPDTVDQLTNTSGNCFAYTPQPDSPPGGSAGSCTAEWGPGTTLTKTGWV
                     FVDMTAPVATGAVADRPPDANGWYNHAVGFSFQGTDATSGIAGCEHPVYGGPDDAGAK
                     IRGRCFDNAGNSSSFTTSLRYDGTAPALSNVKADPGDREVTLSWTAPADTTSVSVTRS
                     APGAPEQVPVARIAAASGPGVTVDRKLHNNRRYSYTLSSTDHAGNVATTSVGARPHAV
                     VLGSGRVRRVLSPPKLKWHKSRRARYYNVQLYRGGRKIFSRWPHGAKLQLSHAWRYRG
                     KAHRLTRGSYHWYVWPGYGKRSAGRYGRLLARGAFRVTR"
     gene            11163..>11994
                     /locus_tag="VF752_05265"
     CDS             11163..>11994
                     /locus_tag="VF752_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854990.1"
                     /translation="MTLVLILATVGMVFAFSGTAAAVPGPGAAPAPLCATPTSPTPGP
                     CSTDWYTEDVKVTWEYDASGTPDPSTPDCLGRTQSTDTTAETFTCTVNWPASGDGEPP
                     PPSPSSSASVTIKVDKTPPTVTEALARPPDYNGWYNHAVDFTFNGTDGAGSGVASCDP
                     VTYSGPEGEGVHVTGTCTDVVGHVGTGSFPLNYDDTLPTLTNVTADDGDSEVTLTWVA
                     STDTRTIDIVRSASGEPDKSIDDVASGAAAGGPGAYTDTTVSNGTHYTYTVIAHDRAN
                     N"
BASE COUNT         1911 a         4224 c         4089 g         1770 t
ORIGIN      
        1 gagacgcgcg ccgaaagtcg tgcggagact atgcgtgcgc gggatcgaca ggcccggagg
       61 gcgccggtag cttcgccggg atgcctgtgg cccgtcttct ctccgtgcat gccgtgctgg
      121 cgggcctggc gctggcctct ctgggcatgg ttgcgcccgc ggctcgggcg gccggcgggg
      181 acatcggcta ccgggatcag tcctacgcgc ccctcggtgg ctcaccgacc agcagcaagc
      241 cggagagcaa gctctggttc aacaacggct ggtgggcgag catgtacaac gcgggcgccg
      301 gcgagcaccg catctacaaa ctcaacagcg ccctcggcgt ctggagcgac agtggcgtgg
      361 cgatcgacaa gcgggcgtcc tcgcgcgcgg acgcgctctg ggacgccggc tcgaagaagc
      421 tctacgtggc gtcgcacgtg tacaccacgt cgggaaccgc aacgaccagc gggagcgcgg
      481 ggcggttgta tcggtacagc tacgacgccg cgggcgaccg ctacgcgctc gatcccggct
      541 tcccggtcac cgtcaacgcg gcgaagacgg agacgctggt gatcgacaag gactcgaccg
      601 gcacgctctg ggccacctgg actcagggca gccgcgtcta cgtgaaccac acgctcggcg
      661 gcaacgacgc cgcctggggc actccgtacg tggtgcccgg gcccggcacg acgctgacga
      721 gcgacgacat ctcgtcggtc atccacttcg gcggcaacaa gatcggcgtg atgtggagca
      781 accaggccga tcacaacttc tacttcagcg tgcacgccga cggggccggc gacgcgtcgt
      841 ggtccacgtc cgccgtcccg agcgcggcgg ccggctccga cgaccacatc aatctgaagg
      901 ccgatagcgc aggtcgtgtc tacgccgccg tcaagaccgc cgcgaacaac aagagccccg
      961 acagtacgcc ggagatcctt ctgctcgtgc ggtcagccgg cggcgcttgg ggcagtgcca
     1021 catttggctc agccgcggac agcgagacgc ggccgatcgt tctgctcgac gagcagcatg
     1081 gcctgattca catgttcgcc acttgtccgc agccgccgag cactagcggc caggcggggg
     1141 gcgacatctg tgagaagacg acgtcgatga gcagcccctc gtttgcggcg ggcataggca
     1201 ccgcggtgat ccgcgacgcc agctccccga agatgaacga cgccacctcc acgaagcagg
     1261 aggtcgacgg cgggaccggg atcgtggtga tggccaacaa cgcgtcgacc gactcctact
     1321 ggcacatgca ggagtcgctg ggggcgcccc cgtccgcgcc ggcgcccgcc cccgtgccga
     1381 agcccgcgcg cacgagcccg cggctcagga tcacctccaa gcgatggcgt ggcttgcgcc
     1441 tctatgtgag gggcacgctg tcgcggaagg cgaccgggcg cgtcaaggtg tcgtacctca
     1501 agggccggcc aggcaagcgc atccgggcgc gcaagagcgc gacgatcaag cgcgggcact
     1561 tcgcggtctc cctgcgcctg cctcgccgcg cacgaggcca caaggcaatt ctctgggcct
     1621 catacggagg agacaggagc tacctggctc aggccgttag ggcacgcgtg cgctgagcac
     1681 gatgcgcgcg gccgctcgtg tcctcgccct tccagtcgcg ctcgcagcct tcgctttcgc
     1741 caccctgggc acggtcgcgc gccctgcgac ggcggccggc ggggacatcg gctaccggga
     1801 ccagtcctac gtacccctcg gcggctcgcc gaccgggagc aagccggaga gcaagctctg
     1861 gttcaacaac ggctggtggg caagcatgtt cagccccgcc gccggcgatc accacatcta
     1921 caagctcgac cgcgcctcgc gggcgtgggc ggataccgga gtcgcgatcg atccgcgcaa
     1981 ctccacccgg gcggatgcgc tgtgggatgc cggctcgcag aagctgtacg tcgcctccca
     2041 cgtctacacg acgtccggaa aagcgacgac cagcgggagc tcgggccggt tgtatcgcta
     2101 cagctacgac gccgcgggtg accgttactc gctcgatccc ggcttcccgg tcaccgtcaa
     2161 ctcggcgaag acagagacgc tggtgatcga caaggactcg accggcacgc tctgggccac
     2221 ctggacccag ggcagccgcg tctacgtgaa ccacacgctc ggcggcggcg acgccgcctg
     2281 gggccgcccg tacatcgtgc ctggctctgg gacgacgctc acgagcgacg acatctcgtc
     2341 ggtcatccac ttcggcggaa acaagatcgg cgtgatgtgg agcaaccagg tcgaccacca
     2401 cttctacttc agcgtgcacg acgacggaag cgacgattca gcgtcgtcct ggtcgacgtc
     2461 ggtcgtgccc gccggagcga gctccgacga ccacatcaat ctcaagacag acagcgccgg
     2521 gcgcgtgtac gcggccgtca agacaagcga gacctcgaag agccggccgc tcgtcctgct
     2581 ccttgtccgg gcggccggcg gaccgtggag cagcgccact tttgggactg tgtcggacag
     2641 ccacacgcgg ccgatcgttc tgctcgacga gcagcacggg ctgatcgaca tgttcgctac
     2701 ctgcccgcag ccgcccaaga cgagcggtca ggccggcgga gacatttgcg agaagacgac
     2761 gtcgatgagc agcccgtcgt tctcgccggg cattggcacg ccgttaatcc gcgaagcggg
     2821 ctcgccgaag atgaacgacg caacctcgac gaaacagaac gtcgacagcg cgacgggcat
     2881 cgtggtgatg gcgaacaacg cctcgaccga tacctactgg cacatgcagt gagttggcgg
     2941 ccgcgccctc cactctgcac cagccgtcgg tcgcgactcg cgcagcatct tcgcggcatc
     3001 ctgcaactgg cagcgatgcc gcgcggcgcc agatccgtgg ctcgagcctg cttctggccg
     3061 gccgcatgct ctccctcgcg gtcaacttcg ccacgcaggt gctcatcgtt cgctacctct
     3121 cgaaggccga cttcggcgcc ttcgcctacg ggctttcgat cgtcaccctc ggcgaaagcc
     3181 tggcagtact cggcctcgac aaggcgatct cgcgcttcct gccgatctac gacgagcaga
     3241 gggcatacgg caagctgttc ggcacgctgc tcatggtcgc cggaacggtg ctctccgtcg
     3301 gcttggcgtt cgtgctgctg gcgttcgggc tccacggatt cgcggggggt gagctcggcg
     3361 ccagcagcca ggcgctggcg gtcatcctca tcctgatctg cctgtcgccc atccaggccc
     3421 tggatgacgt gctgatgggc acgttcgcgg tcttctccaa gccacgcgcg atcttcttcc
     3481 gcaagtacgt cctggcgccc ggcttgcgcc tcacggcgat cctcctcatc gtgctcgccc
     3541 agagcggcgt gcgagagctc gcgttgggtt acgtcatcgc gggcggcctc ggcgtgatgc
     3601 tctacgtggc aatgctcgtc cagacgctgc gcgcggacgg cctgctcgcg cacctccacg
     3661 tgcccgggct gcggttcccg gtgcgcgagg tgttcggctt cgcgctgccg ctcatcacgg
     3721 tcgacctgct gttcgtggtg atgaacacca cgaacgtgtg gatgctcgag cgctggggta
     3781 gcgccgccga cgtggcggac taccgcgtcg tgcagcccgc cgcccggctc aacctgctgg
     3841 tcatgaccag cttcacgctg ctgttcacac ctctcgcggc acggctgttc gcgcgcggcg
     3901 accgccaggg agtccgggag ctgtactggc gcaccgcggc atggatcgcg gtcttctcgt
     3961 tccccgtctt cgcgctcacg ttcgtgtcgg cgcacgagct gacgatcgct ctgttcggtg
     4021 ggcgctacgc cggatccgca ccgatcctgg cgttgctcgc cgccggttac tatttcaacg
     4081 ccgcgctcgg gttcaacgga ctgaccttgc gggtttacgg cctcgtgcgc tacacggtcg
     4141 tgatcagcgt ggcggcggcc gtcgcgaacg tggcaatcaa cttgctgctg attccgcgat
     4201 acggggcgat cggggcgggc gtcggcactt gcgcgaccct gctgatccac aacgtgctga
     4261 agcaggccgg actgctcaag ggaaccggca tcggggtctt caaccgggag tacatgcgcg
     4321 tgtacggcgc gatcgtggtg accatcgccg cactcgtcgc gctgcacgtg ttgctcgcac
     4381 cgggagcgtt cgtcgcgatc gtgctcgtcg cggtcgcctc cgccctcctc ctctggatta
     4441 ctcgtgagac gctgcgagtg ggggacacgt tcccggagct gctgcgcgtc ccgctgcttc
     4501 gacggctcgt gcgctgacag ggttcaagcg gcgaacaaac gacggagacc aggagagtcc
     4561 gcttcgtcga ggctgatcct caggaggttg acgtagtggc cgcggtcgag gcgcgcgggc
     4621 cggaggcgcg ttgcctcctt cagcgcccga tgcatccagc acgtcacgaa cagcggctcg
     4681 acgagctccc gatccagggc gatccgcgcg cactgccgct cgactgccgc gactagcagc
     4741 cggttcaccg gcgtgtccgc cagcaactgg gctcgcaggc cccgcagcga gctcctcacg
     4801 ccggccgcgc gcgccaccgt cacggcgaag ctacgaacga agtagaagac gtcccatagc
     4861 ggcatgccct cgtgctcggc tgcctcccag tcgaggaacg ccggccgtcc cccaggcgtg
     4921 accaggagat tccaggtccc cggatcgccg tgctggagca cgatcggcat ggggcgtgcc
     4981 gcttgcgcca acgcgtcgac gtggcggtgc agccggcttc gctcgaccgc agtgagcgcg
     5041 tagagcgcct cgaagcggcc cagcagatct tcaagcgcag cggcgacgtc ccgatcggcg
     5101 gccgccggat gcgctgtggc tgctccaagt tcgagcagcc actccagcgc cgcgcgggca
     5161 agcgggcagt ccgcttgcgc ggtcgtgcgc tcgcggaagg gcgagcccgc cacggccgaa
     5221 gcgccgagca gcgccagccc gccgtgatga ccgaagaagg cggggtgggg cagtgcgccg
     5281 agcccgtgca ggtcctcatc ctgtagccac tgcagggccc gccactcgtt ctccaaacgc
     5341 gagttgtagg cgccgtcgcg cgtgagcttg accacgagct cgggcaccgg ctcgtcgccg
     5401 cggaagagaa agagcaccgc cttccgcgag gggtactcgg acggcgccca gagggccgcg
     5461 cgatgctcgt cgaggccgac acccgctgcg ccggcgatcg tccgtacata tgtggggact
     5521 ggaagtcggg cctcgtcggc tcccggcacc gcgagcacgc cacgccggct tcttcgtccg
     5581 tgccattgca gcggacggct cgccggtcgc gttctgtgtt gctcgaggta ggcgagcgct
     5641 gagatgtcgc cctcggcaac cgctgcgtgg acttcgccgc gggccggaat catccgcagc
     5701 gcgcaaggtg cgcacacacc cgcctgtagt gcccaggctt cgcggccggc caggtccaag
     5761 aacgccgcct gcgcggcggt cacggcctgg cgaacctcct tgctgccgag cgtggtcgcc
     5821 gcgcgcgcgc cgcccacaac agcgagatcc acctcgcggg cggcctcgta cgcgcgctcc
     5881 ggccgcaaga cccggacgtt cggcgcgccg acctcgacgg cacggcgtcg gacgcggcgt
     5941 cggtcgctct cgcgggtaca gcagacgacc acttctgagg cgagccgcga cagcgtgcgc
     6001 aaggtccgcg ccgacggcgc tccgacgacc agcaccaggc cgagctcgag actcggcagc
     6061 aggaacgtcc acgccgcgcc tgcgccctcg cgttcgcggt tggcgccggc gacgaacgtc
     6121 gacgcgcgcg catgagcgag cacgggagtc tcgtccgcgt gagcggcgga tctcgggcgg
     6181 gcccgcgccg tgacccgcgg gcggaccaac cccagccgtg tccccgggat gcgccagagg
     6241 tgtggcacga ccagcgcgac catccacagt gcgatgagcg cgagcgacct tccgcgcagt
     6301 gtccccaggc ggcgcactac gcccgcctca agccacgcca gtccgcctca tcacgcagcc
     6361 gagcgtctgg atgagcagcc acagctcacg tgtgatcgag cgcctgcgga agaagccgat
     6421 ctcgagacgg caccgttctt cgaagtccag gcagccccgc ccatacacct gccacggccc
     6481 ggtgatgccc gggcggtact ccagacgccc cgtctgccac agggcgtaag tcgacgcgtc
     6541 gaagctcgtc ggacgcgggc ccacgaggct catctcgcca cgcaggacgt tcacgagctg
     6601 gggcaactcg tcgaagctcg agcggcgcag gaagcgcccc acgcgtgtga cgcgggggtc
     6661 gttgacaacc cggaagtcgg gccacggaac gagactgcgc gcgcgcagag catccttgag
     6721 cgcgtcggca tcccgcacca tcgtgcggaa cttgaagagc tggaaggagc gtcccccgta
     6781 gccgcaacgg cgctgccgga agatcacggg cgcgcgcggt tcctcgagcc atacgcacgc
     6841 ggcgatcacg cccagcaatg gcgcgagaag aagcagcgcc ccggcggcca ggcagacatc
     6901 gatggccctc ttcgtgactc gatacgcgac tgtggcctcc gtttatcgcg acgcggctgc
     6961 ccccacgaat cgccgctgat tgacaagatc gaccagctcg aagtccggct gctcaccgaa
     7021 ctcgcgaagc aggcgcactc ggaacgactg gtagtgccgc agcgctcgcg tcttgcggcc
     7081 taggcgcagc caggtgcaga tcaggtcgcg ctggacctcg ctgtcgaaag gctccagctc
     7141 ggcgagccgc tcgagatacg aggacgcctc gaccgggtcg ctcgtccgca acgcggcgag
     7201 ggcccgcagg gcttcgcagg cgagcgcgcg aaggcgctcg cgctcagtca gtgcccacac
     7261 cgcgtagggg tcgtcggcca agaagtcgtc gcggtacagc gcgatggccc gctcgaggag
     7321 ctctgcggcg gcgcagcggt cgccgtgcgc gaaagcgctc aagccggcgg cggcgtcgcg
     7381 ctcgaagtcg tcgacgtcga tccacactcg ttgcgggtcg agcgaatagc cgccgcaccg
     7441 ggacaccaag aacgacgagt cgccgtgctt gggccggcgc ggttccagcc gggtccggag
     7501 cgtatggacg aagtagcgca cgttgcccgg ggcggtagcc ggggcgttcg ggcagatcgc
     7561 ttcggcgatt gcatccgccg aagcggcgcg tgcgcagacg aggtagcgga gcagctgccc
     7621 cggtcgctga tcgagccaat cgtccgaaat cgacccatcg gccgtctcga ccctcagcct
     7681 gccgagcgcg aagatacgca gctgccgctc ggcagcgtcg gtggtcccgt gcgccaagac
     7741 gggcaactga ggagccgctg cgttacgcat gggatgcctt gcgtatgccg ttgcgtatac
     7801 gcgcgcgcgc ccagcgcgca agggatctgc tcgtgtgagt ccggcggccc agcgtcgact
     7861 gaatgctcga gggcctccac tgcgcgccac cccgagccgt cggcacgcct tccttgttga
     7921 gcgtgtctgc gatcgcctgc agcgtcattc cggtctcacg catcgcacga atgcgttgga
     7981 caagctcggg ccgatcggct accgctgggc gactggtccg ccgcgtacct cggacagagg
     8041 gctggtgcct gtttcgcacg cggtcggccc cgcgccaccg ctcgatacgc gtggtgggga
     8101 ggagcgctcg cgcggcggcc gcgccgtcgc gcgtcgtggt atcaagccca acatcgaccg
     8161 caacgaggcc ggcgcgcctg gcggcgaacc agtcgagaag cgaggcgagg tcatacctcg
     8221 agcgcgcgag ctcgtccaac cgcgccacca cgagacgcga ggccgcacca gatgagatct
     8281 gcccgagcgc ctgggcgagg gccggccgcc ggtcggcttt cccgcggtcg cgtgctacgg
     8341 ccaccagcgt ccacccccgc cgcgagcacg cctgctcgat ggcagccgcg tgcccgcctg
     8401 catctgcgtg cctgacatag ccaacggcgc ggggttgcgg tggctcgggc acaggggcag
     8461 gacgggctgg aacgctagga ccgcgccgtt gagccttgcg ggcggagctg cggccctggc
     8521 gtcgtccgcg gcgcctcagt gccgcgactg ttgccgcaca gccggcgccg atcgcaacga
     8581 cggcgacgat cagcagcagc gcaacgagaa gccctctggc tggcttgccg gttttccgca
     8641 gatccgccgg tgtgcgcgcc agctgcttgg tcaccggctg gctgacgacg ggccgcagcg
     8701 gcgccggcgc gtttggcgct tgcgccttcg ctgcgccctt cgcggccgtg gaacggaatc
     8761 gcaggtaggc gagcgtggcg gcgtccgccg cgccggtgcg agcgaagccg tgcttgatct
     8821 ggaaccactg gatcgctgcc ctcgttcgtg ggccgaatag gccgtccacc ggacccggct
     8881 ggtagccgag ggtgcgcagc atcgcctgga tgctgcgcac ccgctcggag ccgcccggcg
     8941 tgtcgtaccc ggccccgaac ctcaccgcgt gcgcgtcagc gctcgtcgct acaaccgccc
     9001 cggaatgctg caacggatcg cggatgggcc ggccgtgcca gctcgcggac gccatcggcg
     9061 gagttcccgc ctgcgtgtct tgaccggcgc ccgtaccgag cgcgctcgcg gggatagcga
     9121 gcgagagcgc gaaaacgaac gtcgcggcca gcgagccgaa catgactcgt gaaaatcgcg
     9181 tgaagagagc cacgcccggc aggtaccccg caggcaacag ggacggccat ggtttcgcga
     9241 gctagagcga cggtatcgag gtcgacgttc ggtgataccg ggctgcgagt cgccagtaga
     9301 gcggcatagc cacggtcgcg acagtctgcg gcggcctccg gggaggacgg cgatatcgcg
     9361 gcgaggtgtc agctggtcgc gctccgcggg gttggttgac gaggtctaga cgttcggcgc
     9421 attgacaggt gcagcaagtc gggcgtactt tggctgcaag gagccgattt tgaaggagga
     9481 ggctccagcg ggaatgtccc tcccacgcag gtacttgcag ttggcggcca tcgcggtggc
     9541 cgcagcaata ctctccacgc cggcgtccgc cctcgccgcc agcccgagtc ccgagcgcgt
     9601 ttcctccggc ggaccgtcgc ccgagcccgc tccatccgcg ggggccgccc agccggcgca
     9661 gggtcaggcg gcgcctgtca cccgttcgcc tgtcactcgg gcggccccag tcggcgcaag
     9721 ggttgccgct ccgtcggcgc ggtcgcagcc ggcggccacg agctccaagc gcgaccgctc
     9781 gcggcaccat cgccgccggc acgccgcacc gcaggtcacg ctcgtgaaga cggggccccc
     9841 gctctacaca tctgctccgc gggcgctctt ccgcgccctt ccggccgccg acaagggcgg
     9901 cgaccggcgc caggcggcga ttctcgcggc cgcggcgctg ttcgcggtcg tggcggcgag
     9961 cgggtcgctg ctgcggatga cgtcgcgcat gaggctgcga tgaagcgcct gctcgtggtc
    10021 ggagccgcgg tcggcgtcgt cgggtggacg atgcccgcaa ccgcgagcgc ttacgacata
    10081 aaggtgtcgt gcacgggatc ctccgggatt ccagtcgcgt gcgggcaatg gcacaacttc
    10141 ccggtcacgc tgcggctggt tcccgacacg gtcgatcagc tcaccaacac gagcggcaac
    10201 tgcttcgcgt acacgccaca accggattcc ccgcctggcg gttcggcggg aagctgcaca
    10261 gcggagtggg ggcccggcac caccctgacg aagacgggct gggtcttcgt ggatatgact
    10321 gcaccggttg ccacaggggc ggttgccgat cgtccacccg acgcgaatgg ctggtacaac
    10381 cacgccgtcg gcttcagctt ccagggcacg gacgcaacct cgggcatcgc ggggtgcgag
    10441 cacccggtat acggcggccc ggacgacgcc ggcgcgaaga tccgcggccg gtgcttcgac
    10501 aacgccggca acagctctag cttcaccacc tcgctccgct acgacggcac ggcgcctgcc
    10561 ctgtcgaacg tgaaggccga ccccggcgac cgtgaggtca cgctgagttg gacggcgccg
    10621 gccgacacga cgagcgtctc agttacccgg tcggcgcccg gggcgccgga gcaggtaccg
    10681 gtggcgagaa tcgccgcggc gagcggcccg ggcgtcaccg tcgatcgaaa gctccacaac
    10741 aaccggcgct acagctacac gctctcctcc acagatcacg ccgggaacgt cgcgaccacg
    10801 agcgtcggag cgcgacccca cgccgtggtg ctcgggagcg gacgcgtgag gcgcgtgctg
    10861 tcgccgccga agctcaagtg gcacaagtca cggcgcgcgc gctactacaa cgtgcagctc
    10921 taccgcggcg gtcggaagat tttcagcagg tggccgcatg gcgccaagct tcagctctca
    10981 cacgcctggc gctaccgcgg caaggcgcac cggctcacgc gcgggagcta ccactggtac
    11041 gtctggccgg gctacggcaa gcgctcggcc ggacgctacg gacggctcct cgcgcgcggc
    11101 gcattccggg tcacgaggtg aaaggtctcg aggtgttccc ccgtctacgc tttcgcgccc
    11161 cgatgacgct cgtgctcatc ctggccaccg tgggcatggt gttcgcgttc tccgggacgg
    11221 ccgccgccgt tcccggtccc ggcgcggctc cggccccgtt gtgcgcgacc ccgaccagtc
    11281 cgacgcctgg accgtgcagc accgactggt acaccgagga cgtaaaggtc acctgggaat
    11341 acgacgcgtc cggaacaccc gacccgagta caccggactg cctgggtcgg actcaatcca
    11401 ctgacaccac cgccgaaacg ttcacatgca ccgtgaactg gccggcctcg ggcgatggtg
    11461 agccgccgcc gccgtcgccg tcgtcatccg ccagcgtgac gatcaaagtc gacaagacgc
    11521 cgcccacagt gacggaggcg ctcgctcggc ctcccgacta caacggttgg tacaaccatg
    11581 ccgtcgattt caccttcaac ggcacagacg gtgcgggatc tggcgtcgcc tcttgcgacc
    11641 ccgtcacata ctcgggtccg gagggtgaag gcgtgcacgt gacgggaacc tgcaccgacg
    11701 tcgtcggcca tgtcgggacc ggcagcttcc cgctgaacta cgacgacacc ctgcccacgc
    11761 tcacgaacgt gaccgcggac gacggcgact ctgaggtgac gctcacgtgg gttgcctcga
    11821 ccgacacgag aaccatcgat atcgttcgtt ccgcatctgg cgagcccgac aagagcatcg
    11881 acgacgtcgc gagcggagcg gctgctggcg gccccggcgc ctacacggac acgaccgtct
    11941 cgaacggcac gcactacacg tacacggtca tcgcacacga tcgcgcgaac aacg
//
LOCUS       DASVXS010000092        13681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1215346, whole genome shotgun sequence.
ACCESSION   DASVXS010000092 DASVXS010000000
VERSION     DASVXS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13681
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215346"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..543
                     /locus_tag="VF752_05270"
     CDS             67..543
                     /locus_tag="VF752_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854991.1"
                     /translation="MRLRLSISALLGLALLLAAAAPAGALIHVQKGIAGVRIGMTQQQ
                     MKDVLGKPRSAKQGSNDFGPYTQFIYPGRIAVTFQGNRRVTGIYTSGTKERTADGVGV
                     GSSERLVKRKVEDVVCETIGSAETCHVGNFDPGHRVTVFLFKDDRVKRVTIGLVLD"
     gene            506..1561
                     /gene="mtnA"
                     /locus_tag="VF752_05275"
     CDS             506..1561
                     /gene="mtnA"
                     /locus_tag="VF752_05275"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX8854992.1"
                     /translation="MSSGSPSGSSSTDELPFQPVRWAGDALELIDQTRLPTEEVWLRC
                     ERPEEVADAIRRLAVRGAPAIGVAAAYGLALGARDGAPERVDEVHELLAATRPTAVNL
                     RWALDRAQSAREGGYEAVLAVAHEIAAEQMRADRAMARHAAALFSEGDRALTHCNTGP
                     LATAGFGTALGALNQAWIDGRLSQVWVDETRPLLQGARLTAWELERLGIPFQVVPDAS
                     AGALMSRGLVDRIVVGADRIAANGDVANKIGTYTLAVLAQRHAVPFYVIAPLSTIDPA
                     TPTGADIPIEERDPSEVTSVGGVPLAPDGAPALNLAFDVTPAELVTAIVTEAGVLEPP
                     YEKSITQALAQVTRGSG"
     gene            complement(1521..2963)
                     /locus_tag="VF752_05280"
     CDS             complement(1521..2963)
                     /locus_tag="VF752_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8854993.1"
                     /translation="MSALEAAIRRPRELAAPRLAWLLAALVAAIFASPWVVLSPDARY
                     SLAWGAQLARGHLPDITDPQVSTDHPLPIAIGGLLSVLGARTAADVYAVLAVLSFFLL
                     VYACFRLGRALAGTAAGVLAAVVLATRPRVDFFAAHSFVDVPFAALVLLATALAVEAR
                     ENGRRVLWLLLLAGLLRPEAWGLTIAWGAWLLWTERREPKRTLGGIAALAIAAPVIWI
                     GFDWIVAGDPLHAVHGTRQGAAFLERTRGVGNLGPTIASAVTAIVGWPLAAAGAVAGA
                     WRLAWAWRAGDRETVRRVGLVLGLALAGFAAFAALAIADLPLNDRYLLVPTLALAVLW
                     ACSLGWLSARSGVVRAVPAGALALGLASVLITAPIDARVTKDMLAASHEKHAADADLE
                     NLVARQEVRRAIDHCPHLIVAGSGRATSAALLGRDPVSIPIGRSAFPPFGTGSLATSD
                     KVEPGFPGSVREGAWVWINRCHGLPGPAPG"
     gene            3023..3964
                     /locus_tag="VF752_05285"
     CDS             3023..3964
                     /locus_tag="VF752_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HEX8854994.1"
                     /translation="MHDAESALSEARRPLVIGIGGGGDVVGALATAELCRVYNGADPV
                     LGGVTWERRVVDPGPGPRRLAEVDGAELMGESVALAGPDTHAGDVVFAEAHMARFVGK
                     PTVLVDPNDGPAAIAEDLMAVANQLGCDLFVFVDVGGDVLATGTEPGLSSPLCDAVLL
                     AAAARIERTGAKVLGGVFGVGCDGELTFDEAMARIAELGRARALGGVRGLTAAIAYRL
                     EQAAREVPTEASALAVRAFHGETGTAEIRGGRRTVELSPVCTLTFYFDPMRAIRSAAP
                     LALAVADAKSLEEANDILNELGVTTELDLEREFAAAG"
     gene            complement(4126..5178)
                     /locus_tag="VF752_05290"
     CDS             complement(4126..5178)
                     /locus_tag="VF752_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX8854995.1"
                     /translation="MTQQVNGHGANGGSPLPTFTKKDFSSDQETRWCPGCGDYAILAT
                     VQSLMPELGVPPERTVFVSGIGCAGRFVYYMDTYGVHGIHGRAPAIATGIAVARPDLS
                     VWVVTGDGDSLSIGGNHLIHALRRNVPLKILLFNNQIYGLTKGQYSPTSEQGKVTKST
                     PFGSQDHPFNPVALALGAEASFVARTVDVDRDHVADTLRAAATHPGSSFVEIYQNCNV
                     FNDGAFDTVREKGLREANQIRLVHGEPIRFGRELERGVARGDDGRLQIVDVGEVGEDA
                     VLVHDAERLEPTLAFELAHLAERPTGPTPIGVFRSVVRPTYAEALGRELAEAHQTADE
                     AALGELLAAGDTWTVS"
     gene            complement(5175..7013)
                     /locus_tag="VF752_05295"
     CDS             complement(5175..7013)
                     /locus_tag="VF752_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX8854996.1"
                     /translation="MTKDVREKERVVVRFAGDSGDGMQLVGSRFTDATAVLGNDLATL
                     PDFPAEIRAPAGTLAGVSAFQIHFASRDILTPGDHPNVLVAMNPAALKANIGALEKGG
                     TVLVNEDAFSKNNLKKAGYADNPLEDGSLADYQLVRVAMTTMTVAATEDIEGITPRDA
                     ARSKNLFALGLISWMYGRPTDVSIEWIEKKFAGKPAICDANLAAFRAGYNFGETAELF
                     AVHYEVKPAPAEPGVYRNVNGTTATALGLIAASVKSRLPLFLASYPITPASELLHELS
                     RHKRFGVRTMQAEDEIAAANIALGAAFGGQLGVTATSGPGLDLKAETIGLAVALELPL
                     VVIDVQRAGPSTGMPTKTEQGDLLTAMYGRHGESPLPIVASSTPGQCFDAALEAVRIA
                     VRYRTPVILLNDTFLANSSEPWRLPSLDDLPDIDPAFATEENTEGGFWPYVRNESLAR
                     PWAVPGTPGLQHRIGGLEKEDLTGNISYDPENHERMTKLREAKVAKVAEGIPALEADR
                     VDGSDVLVLSWGSTYGVARAAVRRVRLSGGKVDHAHLHHLNPMPANTGELLHSYRKVI
                     VPETNMGQLVKIVRADYLVDADSVTKVQGLPFFAEELEEAIRERLA"
     gene            7190..8329
                     /locus_tag="VF752_05300"
     CDS             7190..8329
                     /locus_tag="VF752_05300"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX8854997.1"
                     /translation="MRIGVPNESIGGERRVALVPEVVRRLTGKGGHEVVVEPGAGEAA
                     LIPDSAYEEAGATVGGDVWQADVVVKVAPPTTDEISRLRHGGVLIGFLAPLTSPETSR
                     ALAQQGVTAFAMESIPRITRAQSMDALSSQATVSGYRTALIAAQELGRFFPMLTTAAG
                     TVKPAQVLVLGAGVAGLQAIATARRLGAVVTAFDVRSAVREQVESLGAKFLDLGLESD
                     AEAAGGYARQLTEEEQQAQQTALNEALAKFDVVITTALVPGRPAPRLVTAAGVANMPH
                     GAVIVDLAAEAGGNCELTEPGETVVREGVTIAGPINLPATMPEHASQLYARNLMALIE
                     LITGEDGSLQLDFEDEVVAGAVITRDGEIVHEGAREATMAAQGGS"
     gene            8326..8658
                     /locus_tag="VF752_05305"
     CDS             8326..8658
                     /locus_tag="VF752_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX8854998.1"
                     /translation="MNLVTEIAILVLAAFVGFEVISRVPNTLHTPLMSATNAIHGIVL
                     LGGLLIIGESDGETVNEILGTIAIMFGTINIVGGFLVTERMLEMFRKRPEPKRQAEEP
                     AEEERKAA"
     gene            8658..10076
                     /locus_tag="VF752_05310"
     CDS             8658..10076
                     /locus_tag="VF752_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX8854999.1"
                     /translation="MALPLATSWVQDADFINSLYIVAFSLFIIGLRMLRHPGTAFHGN
                     RVAALGMAIAVGATLLDPRIGNWGLIALGIGLGTAIGIPAARSVKMTAMPQMVALFNG
                     VGGGAVALIAWAEFRHSGGDLATSVIVPTVFAAIIGSISFWGSNIAFAKLQEILSGRP
                     VMLPYQHVINAVLLTGAIAIAVILSAGSDSEALFVGMLVLAAILGNMVVLPIGGADMP
                     VVISMLNALTGLSAAAAGIALDNTALIVAGMLVGASGSILTNLMAQAMNRSIPAIVAG
                     GFGGGGPVGGDDGPALAEGQTVRSTSPDDVAVQLAYASKVIVVPGYGMAVAQAQRAVR
                     DLAVELERRGVDVSYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDEVNPEFPQTDVS
                     LVIGANDVTNPAARSQPDSPIYGMPILDVDKSQSVIVLKRSMNPGFAGIENQLYYEPQ
                     TSMLFGDAKSSVEAVLAELKQL"
     gene            10116..11759
                     /locus_tag="VF752_05315"
     CDS             10116..11759
                     /locus_tag="VF752_05315"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8855000.1"
                     /translation="MARLVLGPLLRHTSDREATIWVETDSACEVEVLDCRDPTFEIAG
                     HHYALACVKGLDPGRTYEYEVRLDGEKVWPEPDSDFPPSVIRTYEDGAPFRLMFGSCR
                     VGYPHEEPYVQASDENKEGREVDALYALALRMKERPNHEWPHALVLLGDQMYADEVSP
                     ATQEFIRSRRDPSKPPGYEVADFEEYTRLYWESWGDPVIRWLLSTVPSCMVFDDHDVH
                     DDWNTSQAWRDEYRAKPWWRSRITGAYMSYWVYQHLGNLAPEHLDTDEFYDRVMKSDD
                     PTRELYEFAVRADESPDGTQWSYRRDFCGNTRLVMIDSRGGRLLEENERAMVDEDEWN
                     WIEGEATGGFDHLLIGTSLPFLLAPGMHHLEAWNEAVCAGAWRRRAAREGERIRQGLD
                     LEHWAAFQRSFRRLAKLIEDVGAGRRGEAPTSILVLSGDVHHAYIADVAFRKGAGVRS
                     RVHQLVCSPFRNPLDKHERAAVNLAMTKPFGRLARALARSAGVEDPPIRWRIDDHWFD
                     NQVASLEFEGRSVNFKLEKTKPEDWPHPKLETVIEQRLA"
     gene            11800..12447
                     /locus_tag="VF752_05320"
     CDS             11800..12447
                     /locus_tag="VF752_05320"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8855001.1"
                     /translation="MGAGDERQEWPRGEARGRIIALLREALADELALARRLRSHIASA
                     PRGPYRAGLERYLRETRSHVTRVGERLAELGEPPSKLRAARSRLVGWLLAPFAALRRR
                     SETKMLASAQFGCATEGLVVATYLALEQLAERAGDTRTADLARGIRLEEQQMLEWLRH
                     EILKLTDSFLMTELDLALGEPPVAVEGRRPPRRWHELGELPADTVRDALAQQERH"
     gene            12448..13083
                     /locus_tag="VF752_05325"
     CDS             12448..13083
                     /locus_tag="VF752_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX8855002.1"
                     /translation="MTEPAGIAFDAFGTLFDLDALSPMLGDAFEGFTSRLVPWTWLVT
                     AAGSFRPLPEVAEAAARAAGADDERVAGTLDALKRLPLKDDVLAGLDALRGRRLAVLS
                     NGTTEGIQALVGNAGLSGRFEHLLTADQVKRYKPAPELYGLAASAFGVPLERVLLVSG
                     NEWDVAAGRLSGMTTAWLARGRELAPVLGVEPHIVADTLPELAEKIERGAL"
     gene            13080..13211
                     /locus_tag="VF752_05330"
     CDS             13080..13211
                     /locus_tag="VF752_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855003.1"
                     /translation="MTAPNERPEWDPDEVPTEEQLDIEGPNEGATDHQPEEEDEDVE"
     gene            13261..13554
                     /locus_tag="VF752_05335"
     CDS             13261..13554
                     /locus_tag="VF752_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855004.1"
                     /translation="MTEERDQAKEKIHELEEGDPPEKLEDWPDDDAKYETYGGPEGEH
                     SYDEGPESKLGPSSVRHHEDGEVSVEGEKVENPEDFKGDPIPGGPTDPDAPAA"
BASE COUNT         2139 a         4688 c         4871 g         1983 t
ORIGIN      
        1 gggccagctc cacagctgcc tgaggcggtc gcaagaagca cgctcgtggg ctaccttgga
       61 cgcgcgatga ggctccgtct ctcgatctca gcgctactcg gactcgcgct gctgctcgcc
      121 gcggcggcac cggcaggcgc cctgatccac gtccagaagg gcatcgccgg ggtgcgtatc
      181 ggcatgaccc agcagcagat gaaggacgtt ctcgggaagc cgcgctccgc gaagcaggga
      241 tcgaacgact tcgggcccta cacgcagttc atctatcccg gccggatcgc cgtcacgttc
      301 caggggaatc gaagggtcac gggcatctac acgagcggga cgaaggagcg caccgccgac
      361 ggcgtgggcg tcggctcgag cgagcgcctc gtgaagcgca aggtggagga cgtggtctgc
      421 gagacgatcg gcagtgcgga aacctgtcac gtcggcaact tcgatccagg ccatcgcgtg
      481 acggtgttcc tcttcaagga cgaccgtgtc aagcgggtca ccatcgggct cgtcctcgac
      541 tgacgagctt cccttccagc cggtccgctg ggccggtgac gcgctcgagc tgatcgacca
      601 gacgcgcctg cccaccgagg aggtgtggct ccgctgcgag cgacccgagg aggtggccga
      661 cgcgatccgc cgcctggcgg ttcgaggagc gcccgccatc ggcgtggcag ccgcctacgg
      721 gctggcgctc ggagcgcgcg acggtgctcc tgagcgcgtc gacgaggtcc acgagttgct
      781 cgccgcaacg cggccgacgg cggtgaacct gcgctgggcg ctcgaccgcg cgcagtcggc
      841 gcgcgagggc ggctacgagg cggtgctggc ggtcgcgcac gagatcgcag ccgagcagat
      901 gcgcgcggat cgcgccatgg cacgccacgc cgccgcgctc ttctcggagg gcgaccgggc
      961 gctcacccac tgcaacaccg ggccactggc gacagccgga ttcggcacgg cgctgggcgc
     1021 gctcaaccag gcgtggatcg acggccgcct gtcgcaggtg tgggtggacg agacgcgacc
     1081 gctgcttcag ggcgcgcggc tgacggcgtg ggagctggag cggctcggga ttcccttcca
     1141 ggtggtgccc gacgcgagcg cgggcgcgct gatgtcgcgc gggctcgtgg accggatcgt
     1201 ggtaggcgcc gaccgcatcg ccgccaacgg cgacgtggcc aacaagatcg gcacctacac
     1261 gcttgccgtg ctggcacagc ggcacgccgt gccgttctac gtgatcgccc cgctttccac
     1321 gatcgatccc gcgactccga ctggcgccga catcccgatc gaggagcgcg acccttccga
     1381 agtcacgagc gtcggcggcg tgccgctcgc acccgacggc gcaccagcgc tcaacctggc
     1441 cttcgacgta acgccggccg agctggtcac cgccatcgtg acggaagcag gagtgctaga
     1501 gccgccgtac gagaagtcga tcacccaggc gctggcccag gtaacccgtg gcagcggttg
     1561 atccagaccc acgccccctc gcggacgctc cccgggaagc caggctccac cttgtcggag
     1621 gtcgcgagcg acccggtgcc gaagggcggg aacgcgctgc ggccgatcgg gatcgagacc
     1681 ggatcgcgac cgagcagcgc ggctgacgtc gcgcgcccgc tgccggcgac gatcaggtga
     1741 ggacagtggt cgatcgcgcg ccgcacctcc tgccgggcga cgaggttttc gaggtcggcg
     1801 tcggccgcgt gcttctcgtg cgaggcggcg agcatgtcct tcgttacccg cgcgtcgatc
     1861 ggcgcggtga tcagcacgga cgcaaggcca agggcgagcg cgcccgccgg caccgcgcgg
     1921 accactcccg accgcgcgga gagccagccg agcgagcacg cccacaggac ggcgagcgcg
     1981 agcgtcggca cgagcaggta gcggtcgttc agcggcaggt cggcaatggc gagcgccgcg
     2041 aaagcggcga aaccggcgag cgcgaggccg agcacaagcc caacgcggcg cactgtctcg
     2101 cggtcgcccg cgcgccacgc ccaggcgagt cgccacgctc cggccactgc tcccgccgcg
     2161 gcgagcggcc agccgacgat cgctgtcacc gccgaggcga tggtcggccc gagattgcct
     2221 acgccgcgtg tgcgctcgag gaacgcggcg ccctggcggg tgccgtgcac cgcgtggagc
     2281 gggtcgccgg ccacgatcca gtcgaagccg atccagatga cgggcgcagc gatcgccaac
     2341 gccgctatac ccccgagcgt tcgcttcggc tcgcgccgct cggtccacag cagccaagcc
     2401 ccccaagcga tcgtgaggcc ccatgcctcg ggccgcagga gccccgcgag caggagcagc
     2461 cagagcacgc gccgcccgtt ctcgcgcgcc tcgacggcaa gcgcggtagc gagcagcacg
     2521 agcgccgcga acggaacgtc gacgaacgag tgcgccgcga agaagtcgac gcgcgggcgc
     2581 gttgccagaa cgactgcggc gagcacgcct gcggcggtgc cggcgagcgc gcggccgagc
     2641 cggaagcacg catacacgag caggaagaag gacagcaccg ccagcacggc gtagacgtcg
     2701 gctgccgtgc gcgctccgag caccgagagc agcccgccga tcgcgatcgg cagtgggtgg
     2761 tcggtcgaaa cctgcggatc ggtgatgtcg ggcaggtggc cgcgcgcgag ctgcgcaccc
     2821 caggcgagcg agtagcgcgc gtccggcgaa agcacgaccc agggactcgc gaagatggcc
     2881 gcgaccagcg cggcgagcag ccacgcgagc cgcggggccg cgagctcacg cggccttcgg
     2941 atcgcagcct cgagcgcgct catcgcgccg aattctggcg gcgggcggtt cgccgcgggc
     3001 gcacgtgggc aacaatccgc cagtgcacga cgccgaatcc gcactgtccg aggctcgccg
     3061 cccgctcgta atcgggatcg gtggcggcgg cgacgtcgtc ggcgccctcg ccaccgcgga
     3121 gctctgtcgt gtctacaacg gggccgaccc ggtgctgggt ggagtcacct gggagcggcg
     3181 cgtcgtggac cccgggcccg gtccgcgccg gctcgccgag gtggacggag ccgagctgat
     3241 gggagagagc gtcgcgcttg ccggaccgga cacgcatgcg ggcgacgtgg tgttcgccga
     3301 ggcgcatatg gcgcgcttcg tcggcaagcc gacggtgctg gtggacccga acgacggacc
     3361 agccgcgatc gccgaagacc tgatggcggt cgcgaatcaa ctcggttgcg acctgtttgt
     3421 gttcgtggac gtgggcggcg acgtgctagc cacgggcact gagcccggcc tgtcgagccc
     3481 gctgtgcgac gccgtgctgc tggcggccgc tgcgcgcatc gagcgcacgg gcgcaaaggt
     3541 gctcggcggc gtcttcggag tcggctgcga cggcgagctg actttcgacg aggcgatggc
     3601 acgaatcgcg gagctcgggc gcgcccgtgc gcttggcggc gtgcgcggac tcaccgctgc
     3661 gatcgcctac cggctggagc aggccgcgcg cgaagtgccg accgaggcaa gcgcgctcgc
     3721 ggtacgcgcc ttccacgggg agaccggcac cgcggagatc cggggcgggc gccgcaccgt
     3781 cgagctctcc cccgtgtgca cgctcacctt ctacttcgac ccgatgcgcg cgatccgctc
     3841 ggcggcgcca ctcgcgctgg ccgtggcaga cgcgaagagt ctcgaggagg cgaacgacat
     3901 cctcaacgag cttggcgtga cgacggagct cgatctggaa cgcgagttcg cggcggcggg
     3961 ttaggcaata acgacagctg ggccagcgcc gtcgtcactt cgggcccata gtgggcccaa
     4021 atcgacgaca caagccactt ggtcccgaac ggcgacccgc cgactgccgg atcgccgtca
     4081 ttgctaattc gggggtagtc ggggcgaacc gggcgcgcgc gccgtctacg aaacggtcca
     4141 ggtatcgccg gcagcgagaa gctcccccag cgccgcctcg tcggctgtct ggtgcgcctc
     4201 ggccagctcg cgccccagcg cctccgcata cgtcggccgc accaccgagc ggaacacgcc
     4261 gatcggagtt ggcccggtcg ggcgctcggc caggtgcgcc agctcgaagg cgagcgtcgg
     4321 ctcgagccgc tctgcgtcgt gcacgaggac cgcgtcctcg cccacctcgc ccacgtccac
     4381 gatctgcaga cgcccgtcgt cgccgcgcgc aaccccgcgt tcgagctcac gcccgaagcg
     4441 gatcggctca ccgtgcacga gacggatctg gttcgcctct cgcaggccct tctctcgcac
     4501 ggtgtcgaag gcgccgtcgt tgaacacgtt gcagttctgg tagatctcga cgaacgagga
     4561 gcccgggtgc gtggcggccg cgcgcagcgt gtcggccaca tggtcgcgat cgacgtccac
     4621 ggtgcgcgcc acgaacgagg cctcggcgcc gagcgccagc gcgacggggt tgaagggatg
     4681 gtcctgcgag ccgaacggcg tggacttcgt caccttcccc tgctcgctgg tcggcgagta
     4741 ctggcccttg gtgagcccgt agatctggtt gttgaagagc aggatcttca gcggcacgtt
     4801 gcggcgcagc gcgtggatca ggtggttgcc gccgatcgag agtgagtcgc cgtcgcccgt
     4861 cacgacccac accgacaggt cgggccgcgc gacagcgatg ccggtcgcga tcgccggcgc
     4921 acggccgtgg atgccgtgga cgccgtaggt gtccatgtag tagacgaagc ggccggcgca
     4981 gccgatgccg gacacgaata cggtccgctc cggcggtacg cccagctctg gcatcagcga
     5041 ctggaccgtg gcgaggatcg cgtagtcgcc gcagcccggg caccagcgcg tctcctggtc
     5101 gctcgagaag tccttcttcg tgaaggtcgg tagcggcgag ccgccgttcg cgccgtggcc
     5161 gttcacctgc tgcgtcatgc gagccgctcc cttatcgcct cttccagctc ctccgcgaag
     5221 aacgggaggc cctgcacctt cgtcacgctg tcggcgtcca cgaggtagtc cgcgcgcacg
     5281 atcttcacga gctgacccat gttcgtctcc ggcacgatca ccttccggta ggagtggagc
     5341 agctcgccgg tgttcgccgg catcgggttg aggtggtgga gatgcgcgtg gtcgactttc
     5401 ccgcctgaga gccgtacgcg cctcaccgcc gcgcgcgcga cgccgtacgt cgagccccag
     5461 ctgagcacga gcacatccga gccgtcgacg cggtctgcct cgagcgccgg gatcccctcc
     5521 gccaccttcg ccaccttggc ctcgcgcaac ttcgtcatgc gctcgtggtt ctcggggtcg
     5581 tagctgatgt tgcccgtgag gtcttccttc tcgagcccgc cgatgcggtg ctgcagaccc
     5641 ggcgtgcccg gcacggccca gggccgcgcg agcgactcgt tgcgcacgta cggccagaag
     5701 ccgccctcgg tgttctcctc ggtggcaaag gcgggatcga tgtcgggcag gtcgtccagg
     5761 gacggcagcc gccacggctc cgacgagttc gccaggaagg tgtcgttgag caggatcacc
     5821 ggcgtgcggt agcggacggc gatccgcacc gcctcgagcg cggcgtcgaa gcactgaccc
     5881 ggtgtcgagg aggcaacgat cggcagcgga gactcgccgt gtcgcccata catcgcggtg
     5941 aggagatcgc cctgctcggt cttggtgggc atgccggtcg agggcccggc gcgctgcacg
     6001 tcgatcacga ccagcggcag ctcgagcgcg accgccagcc cgatcgtctc cgccttcagg
     6061 tcgagtcccg ggccgctcgt cgcggtcact cccagctggc cgccgaacgc cgcgccgagc
     6121 gcgatgttcg ctgccgcgat ctcgtcctcc gcctgcatcg tgcgcacgcc gaagcgcttg
     6181 tggcgcgaga gctcgtgcag cagctccgac gccggcgtga tcgggtagct cgcgaggaac
     6241 agcggcagcc ggctcttcac gctcgcggcg atcagcccga gcgccgtcgc cgtcgtgccg
     6301 ttcacattgc ggtacacccc cggctcggcc ggcgcgggct tcacctcgta gtggacggcg
     6361 aagagctcgg cggtctcgcc gaagttgtag cccgcgcgga acgccgcgag gttcgcgtcg
     6421 cagatcgccg gcttgcccgc gaacttcttc tcgatccact cgatcgagac gtcggtcgga
     6481 cggccgtaca tccaggagat caggccaagg gcgaagaggt tcttcgagcg cgcggcatcg
     6541 cgcggggtga tgccctcgat gtcctcggtc gcagcgactg tcatggtcgt catcgcgacg
     6601 cgcaccagct ggtaatccgc cagcgacccg tcctcgagcg ggttgtcggc ataacccgcc
     6661 ttcttgaggt tgttcttgct gaaggcgtcc tcgttcacga ggactgtgcc gcccttctcg
     6721 agcgcgccga tgttcgcctt gagcgccgcc gggttcatcg cgacgaggac gttcgggtga
     6781 tcgcctggcg tgaggatgtc gcgcgaggcg aagtggatct ggaaggcgga aacgccggcg
     6841 agggtgccgg cgggagctcg gatctcagcg gggaagtccg gaagcgtggc gaggtcgttg
     6901 ccgagcaccg ccgtggcgtc cgtgaagcgg ctcccgacga gctgcattcc atcgcccgag
     6961 tcgcccgcaa agcggacaac tactcgctct ttttccctga cgtcctttgt cacggtggcg
     7021 tgccgtctcg acggtggggc cggagtcgga cacgtacttc ttattccggg taaggacgat
     7081 tgtattccgg caatcggcgg ccacccgagc gcattcgcgc cagcggggcg ggagcgcgcc
     7141 ttccggcgcg tttcattacg ctccccggtc cctccggggg gttattgact tgaggattgg
     7201 ggttccaaac gagtccatag ggggcgagcg gcgcgtcgcg ctcgtgcccg aggtcgtgcg
     7261 ccgactcacc ggcaagggcg gccacgaggt ggtcgtcgag cccggcgcgg gcgaggccgc
     7321 cctaatcccc gattccgcgt acgaggaggc cggcgccacc gtcggcggcg acgtctggca
     7381 ggccgacgtg gtggtgaagg tcgctccgcc gactacggac gagatctctc gcctcagaca
     7441 cggaggcgta ttgatcggct tcctcgcgcc gctcacgtcg cccgagacct cgcgcgcgct
     7501 cgcccagcag ggcgtgaccg ccttcgcgat ggagtcgatc ccgcgcatca cgcgcgcgca
     7561 gtcgatggac gcgctctcct cccaggcgac cgtctccgga taccggacgg cgctgatcgc
     7621 ggcccaggaa ctcggccgct tcttcccgat gctcaccacg gcggccggca cggtgaagcc
     7681 ggctcaggtg ctggtgctcg gcgccggcgt cgccggcttg caggcgattg ccaccgcgcg
     7741 ccggctcggc gcggtggtga ccgcattcga cgtccgctcg gcggtgcgcg agcaggtgga
     7801 gtcgctcggg gcgaagttcc tcgacctcgg cctcgagagt gacgccgagg cggcgggcgg
     7861 ctacgcgcgc cagctcaccg aggaggagca gcaggcccag cagacggcgc tgaacgaggc
     7921 gctcgcgaag ttcgacgtgg tgatcacgac agcgctcgtg ccgggccggc ccgcaccacg
     7981 cctcgtgacc gccgccggcg tggcgaacat gccgcacggc gcggtgatcg tcgacctcgc
     8041 cgcggaggcc ggcggcaact gcgagttgac cgagcccggg gagacggtcg tgcgcgaggg
     8101 cgtgacgata gccgggccga tcaacctgcc ggccacgatg cccgagcacg cgtcccagct
     8161 ctacgcacgc aacctgatgg cgctgatcga gctcatcacc ggcgaggacg gctccctgca
     8221 gctcgacttc gaggatgaag tcgtcgccgg cgcggtaatc acgcgcgatg gagagatcgt
     8281 tcacgaaggt gcccgcgaag cgacaatggc cgcccaggga ggctcatgaa cctcgtcacg
     8341 gagatagcaa tcctcgtact ggccgccttc gtcggcttcg aggtgatctc gcgcgtaccg
     8401 aacacgctcc acaccccgct gatgtcggcc acgaacgcca tccacggcat cgtgctgctc
     8461 ggcggcctgc tgatcatcgg tgagtcggat ggcgagaccg tcaacgagat cctcgggacc
     8521 atcgcgatca tgttcggcac gatcaacatc gtcggcggct tcctggtgac cgagcgcatg
     8581 ctcgagatgt tcaggaagcg tcccgagccg aagagacagg cggaagagcc ggccgaagaa
     8641 gagcgaaagg cggcgtagtg gcccttccgc tcgcgacgtc ctgggtgcag gacgcggact
     8701 tcatcaacag cctctacatc gtcgcgttct cgctgttcat catcgggctg cggatgctgc
     8761 gccaccccgg cacggcgttt cacggcaacc gggtggccgc gctcggcatg gcgatcgcgg
     8821 tcggcgcgac gctgctcgac ccgcggatcg gcaactgggg cctgatcgcg ctcggcatag
     8881 gactgggcac ggcgatcggc atcccggccg cgcgctcggt gaagatgacc gcgatgccgc
     8941 agatggtcgc gctgttcaac ggcgtcggcg gcggtgcggt ggcgctgatc gcctgggcgg
     9001 agttccgcca ctcaggcggc gacctcgcca ccagcgtgat cgtccccacg gtgttcgcgg
     9061 cgatcatcgg ctcgatctcg ttctggggct cgaacatcgc attcgcgaag ctccaggaga
     9121 tactcagcgg gcggccggtc atgcttccgt accagcacgt gatcaacgcg gtgctgctca
     9181 cgggagcgat cgcgatcgca gtcatcctct ccgccggctc ggattccgag gcgctgttcg
     9241 tgggcatgct cgtgctcgcg gcaatcctcg ggaacatggt cgtgctaccg atcggcggag
     9301 cggacatgcc cgtggtcatc tcgatgctga atgccttgac cggcctctcg gctgccgcgg
     9361 ccggtatcgc gctcgacaac accgcgctga tcgtggcggg catgctcgtg ggcgcctccg
     9421 gctcgatcct cacgaacctg atggcgcagg cgatgaaccg ctcgatcccg gcgatcgtgg
     9481 ccggcggctt cggcggaggc ggcccggtcg gtggagacga cgggccggcg ctcgcggagg
     9541 gccagaccgt gcgctcgacg agcccggacg acgtcgcggt acagctcgcc tatgcctcga
     9601 aggtgattgt cgtccctggc tacggcatgg ccgtcgccca ggcgcagcgc gctgtgcgcg
     9661 atctcgccgt cgagctcgag cgccgcggcg tcgacgtctc ctacgcgatc catccggtcg
     9721 ccggccgcat gccgggccac atgaacgtgc tgctcgccga agccgacgtg ccgtacgagc
     9781 agctcaagga gatggacgag gtcaacccgg agttcccgca gaccgacgtg tcgctcgtga
     9841 tcggcgcgaa cgacgtgacc aatcccgccg cccggagcca gccggacagc ccgatctacg
     9901 gcatgccgat cctggacgtg gacaagtcgc agtcggtgat cgtgctcaag cgaagcatga
     9961 acccgggctt cgcgggcatc gagaaccagc tgtactacga gccgcagacc tcgatgctgt
    10021 tcggcgacgc gaagtcgtcg gtcgaggcgg tgctcgccga gctgaagcag ctctagcgcg
    10081 cataaccccc gccgcacctc gggtaggacc cgggcatggc ccggctcgta ctaggtccac
    10141 tgctccgcca cacgagcgac cgcgaagcga ccatctgggt agagacggat tccgcctgcg
    10201 aggtggaggt gctcgactgc cgcgacccga ccttcgagat cgccggccac cactacgcgc
    10261 tcgcgtgcgt gaaggggctc gatccggggc gcacctacga gtacgaggtg cggctcgacg
    10321 gcgagaaggt ctggcccgag ccagattctg acttcccgcc gagcgtgatc cgcacctacg
    10381 aagacggcgc gccgttccgg ctcatgttcg gctcgtgccg cgtcggctac ccgcacgagg
    10441 agccgtacgt gcaggcgagc gacgagaaca aggaggggcg cgaggtcgac gcgctctacg
    10501 cactcgctct gcggatgaag gaacgcccca accacgagtg gccgcacgcg ctcgtgctgc
    10561 tcggcgacca gatgtacgcg gacgaggtct ctcccgccac gcaggagttc atccgctcgc
    10621 gccgcgaccc ctccaagccg cccgggtacg aggtcgccga cttcgaggag tacacgcgtc
    10681 tctactggga gtcctggggc gaccccgtga tccgctggct gctctccacc gtgccgagct
    10741 gcatggtgtt cgacgaccac gacgtgcacg acgactggaa cacctcccag gcgtggcgcg
    10801 acgagtatcg cgcgaagccc tggtggaggt cgcgcatcac cggcgcctac atgagctact
    10861 gggtctacca gcacctcggg aacctcgcac cggagcacct cgacaccgac gagttctacg
    10921 accgcgtgat gaagtcggac gaccccacgc gcgagctcta cgagttcgcc gtgcgcgcag
    10981 acgagagtcc ggacggcacg cagtggagct accggcgcga cttctgcggc aacacgcggc
    11041 tcgtgatgat cgactcgcgg ggcggccgcc tgctcgagga gaacgagcgc gcgatggtcg
    11101 acgaggacga gtggaactgg atcgagggcg aggcgacggg cggcttcgac cacctgctga
    11161 tcgggacgtc gctgccgttc ctgctcgccc ccggcatgca ccacctcgag gcctggaacg
    11221 aggcggtgtg cgcgggcgca tggcgacggc gtgccgccag ggagggagag aggatccgcc
    11281 aagggctcga cctcgagcac tgggccgcgt tccagcgctc gttccggcga ctggcgaagc
    11341 tgatcgagga cgttggcgcg ggccgccgcg gcgaggcgcc cacgagcatc ctggtcctct
    11401 cgggcgacgt ccaccacgcg tacatcgccg acgtcgcgtt tcggaagggc gccggcgtgc
    11461 gcagccgcgt gcaccagctc gtctgctcgc ccttccgcaa cccgctcgac aagcacgagc
    11521 gcgcggcggt gaacctcgcg atgacgaagc ccttcggccg gctcgcgcgg gcgctcgcgc
    11581 gctcggccgg cgtggaggat ccgccgattc gctggcggat cgacgaccac tggttcgaca
    11641 accaggtggc gtcgctcgag ttcgaaggcc gctccgtgaa cttcaagctc gagaagacga
    11701 aaccggagga ctggccgcac ccgaagctcg aaacggtgat cgagcagcgc ctggcgtaat
    11761 gctcgacacc gaccgccgag ggagtatttt tcccggtcga tgggcgcagg ggatgagcgg
    11821 caggagtggc cgcgcggaga ggctcgcggg cgcataatcg cgctccttcg cgaggcgctc
    11881 gcggacgagc tcgcgctggc caggcggctt cgttcccaca tcgcctcggc gccgcgcggc
    11941 ccctaccggg ccgggctcga gcgttacctg cgcgagacgc gctcccacgt gacccgcgtg
    12001 ggcgagcggc tcgccgagct cggagagccg ccgtcgaagc tgcgcgctgc gcggagccgc
    12061 ctcgttgggt ggctgctcgc gccgttcgct gcgctgcggc gccgcagtga gacgaagatg
    12121 ctcgcgagcg cgcagttcgg ctgcgcgacg gagggcctag tggtcgcgac atacctcgcg
    12181 ctcgagcagc tcgccgaacg cgccggcgac acgcgcaccg ccgaccttgc gcgcggcatc
    12241 cgcctcgagg agcagcagat gctcgagtgg ctgcggcacg agatcctcaa gctcaccgac
    12301 tcgttcctga tgaccgagct cgacctggcc cttggcgagc cgccggtggc ggtggaggga
    12361 cgccgcccgc cgcggcgctg gcacgagctc ggcgagctgc cggcggatac tgtgcgtgac
    12421 gcactcgcgc agcaggaacg gcactgagtg actgagccgg ccgggatcgc gttcgacgca
    12481 ttcggcacgc tcttcgacct ggatgccctc tcaccgatgc tgggcgacgc cttcgagggg
    12541 ttcacgagcc ggctcgtgcc gtggacctgg ctggtcacgg ccgccgggtc gtttcggccg
    12601 ctcccggagg tcgccgaggc ggcggcgcga gccgctggag cagatgacga acgggtcgcg
    12661 gggacgctcg acgcgctgaa gcgcctgcca ttgaaggacg acgtcctcgc ggggctcgac
    12721 gcgctccgcg gccgccgtct cgcggtcctc tcgaacggca ccactgaggg gattcaggcg
    12781 ctggtcggga acgccggcct gagcggccgc ttcgagcacc ttctcaccgc cgaccaggtg
    12841 aagcgctata agcccgcgcc cgagctgtac gggctcgccg cgagcgcgtt cggggtgccg
    12901 ctcgagcgcg tgctgctcgt cagcggcaac gagtgggacg tggccgccgg gcggctctcg
    12961 ggtatgacaa cggcgtggct ggcgcgcggg cgagagctcg cgccggtgct gggcgtggag
    13021 ccgcacatcg tggccgatac gctgcctgag ctggcggaga agatcgaaag gggagcgcta
    13081 tgacagcccc gaacgaacgc cccgagtggg atcccgacga ggtgccgacc gaggagcagc
    13141 tcgacatcga aggcccgaac gagggcgcca ctgaccacca gccggaagaa gaggacgagg
    13201 acgtggagtg atctcacccg tccggggtat gcctctctct ggtctacaga gggaggcgac
    13261 gtgacggagg agcgagacca ggccaaggag aagatccacg agctcgagga aggggatccg
    13321 cccgagaagc tcgaggactg gccggacgac gacgcgaagt acgagaccta cggcggcccc
    13381 gagggcgagc actcgtacga cgaaggacct gagtcgaagc tcggcccatc gtcggttcgc
    13441 caccacgagg acggcgaggt gagcgtggag ggcgagaagg tcgagaaccc tgaggacttc
    13501 aagggggatc cgatcccggg cgggcccact gatccggatg cgcccgccgc gtgaacagcg
    13561 gcaataaaga gtgaactccc tcagctgggc tagctcgatc taggcgtggg tgaggttagg
    13621 gtcgaatagc gacccaaagg tcacccaccc cctctcgagc actccctaag agcgtgtttt
    13681 t
//
LOCUS       DASVXS010000093         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_944464, whole genome shotgun sequence.
ACCESSION   DASVXS010000093 DASVXS010000000
VERSION     DASVXS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944464"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2080)
                     /locus_tag="VF752_05340"
     CDS             complement(<1..2080)
                     /locus_tag="VF752_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX8855005.1"
                     /translation="MAETSSVVASSPVSRDDLDRAEKFAREPFLHEGEEASAVLAPGS
                     PRRRALDAALEEIDQGRDYPSVEWRQEYSLMLGLERLLNEEEPHLADGTVLNAHQVDA
                     LSGTLIALLSELQVSARNGNGRNGQYEELPSGEEELEGDEELGDDEEPLDWDPGAERV
                     DEDEQLPEAPEDPGSSRRFWFEHATGAGKTVAALGFVEASRTGGVLILTHRRNLVDQF
                     HGELFDRGYKKRVTPALLKNDRVPDVGAGVVTVETYQWFVRNAGQVSDAYTIVICDEA
                     HTALGEKTSETIRRWQGPTFIGMTATGALIARHVTDLFPTQTSRFDLAQAARRGVIAP
                     LRCVRIPPGVGVRTIAKVPLRKGEVDQDFDQEELAALLDQGPFNAAVADLYRVRFKDL
                     PGVVYAAGVRHAYNVAKAFQDAGMKARAVSGETPKRELTRVLSAYERGEIDVLVNAQL
                     LAEGWNSPRATICMHLAPTASRRVYQQRVGRVTRRNPGKEAGIVVDFVHPATTHDDPV
                     VTLHSLLDRDVYRGGAIVVGPVRRGRGRRIRVERRVVPVTADTDKRAQVFERELWRIA
                     VENLSWAEQHAWAALAGARVASNNWRRAKAMLHFDQTGELRRRYLITCLQRNRNSQLR
                     IRALQEVAALRDAEAFDEAVDIVGTWSRDERREAVKVLLQALAERSIGRRDQATAWLW
                     RLAEYTREVHE"
     gene            2301..2891
                     /locus_tag="VF752_05345"
     CDS             2301..2891
                     /locus_tag="VF752_05345"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8855006.1"
                     /translation="MNRRRVITVLGAAALIAVVVIGLISAGGSGPSSSPKSAAPSATQ
                     VSKELAGAPAPLAALHNQANQLLPGGASAFQKRLAELRGHPVVVNKWASWCGPCRAEF
                     PHFQQASVEHGKQVAFIGVDSSDNNGQAQAFLKKFPVSYPSYEDGDLKIAQKLNAVGA
                     FPTTVYYDSKGRRVGEHIGVYQKASDLESDIKRYAQ"
     gene            2916..>3473
                     /locus_tag="VF752_05350"
     CDS             2916..>3473
                     /locus_tag="VF752_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855007.1"
                     /translation="MPELRIDPLSGLRTIVAGERASRPGGGFHADPQPPLDPESDPFL
                     EGHEDRTPPEVFALRPNGGAPNTPGWVVRVVPNLYPALSEDASARATADPLELGRGEP
                     ELFATSPAHGAHEVIVNAPDPVTSLRALAPEQVETAMGVWRERIRVHSDASVVHVLVN
                     EGREAGASLPHTHAQLYALPFVPAQV"
BASE COUNT          488 a         1218 c         1240 g          527 t
ORIGIN      
        1 cctcgtgcac ctcgcgcgtg tactcggcga ggcgccagag ccaggcggtg gcctggtcgc
       61 ggcggccgat cgagcgctcc gcaagagcct gcagcagcac cttcacggcc tcgcggcgct
      121 cgtcgcgcga ccaggtgccg acgatgtcga ccgcctcgtc gaacgcctcg gcgtcgcgca
      181 gcgcggcgac ctcctgtagc gcgcggatgc gcagctgcga gttgcggttg cgctgcaggc
      241 aggtgatcag gtagcgacgg cgcagctcgc cggtctggtc gaagtggagc atcgccttgg
      301 cgcggcgcca gttgttcgag gcgacgcgcg cgccggcgag cgcggcccag gcgtgctgct
      361 cggcccacga caggttctcg accgcgatcc gccacagctc gcgctcgaac acctgcgcgc
      421 gcttgtcggt gtcggcggtg acgggcacca cgcggcgctc cacgcggatg cgccggccgc
      481 ggccgcggcg caccggcccg accacgatcg cgccgccgcg gtagacgtcg cggtcgagca
      541 gcgagtgcag cgtgaccacc gggtcgtcgt gcgtggtggc cgggtgcacg aagtcgacga
      601 cgatgcccgc ctccttgccc gggttgcggc gcgtgacgcg gccgacgcgc tgctggtaga
      661 cgcggcggga ggcggtcggc gcgagatgca tgcagatcgt ggcgcgcggc gagttccagc
      721 cctcggcgag cagctgtgcg ttcacgagca cgtcgatctc gccgcgctcg tatgcggata
      781 gcacgcgcgt cagctcgcgc ttcggtgtct cacccgacac cgcgcgcgcc ttcatgcctg
      841 cgtcctggaa ggccttcgcg acgttgtagg cgtggcggac gccggcggcg tagacgacgc
      901 ccggcaggtc cttgaagcgc actcggtaga ggtcggccac ggccgcgttg aacgggccct
      961 ggtcgagcag tgcggccagc tcttcctgat cgaagtcttg gtccacctcg cccttacgga
     1021 gcggcacctt ggcgatcgtt ctcacgccca cgcccggcgg gatgcgcaca catcgaagcg
     1081 gcgcaatcac gcctcggcgc gcggcctggg cgaggtcgaa gcgcgacgtc tgggtcggga
     1141 agaggtcggt gacgtgccgc gcgatgagcg cgccggtggc cgtcatgccg atgaaggtgg
     1201 ggccctgcca gcggcggatc gtctcgctcg tcttctcgcc gagagccgtg tgagcctcgt
     1261 cgcagatgac gatcgtgtag gcgtccgaga cctgccccgc gttgcgcacg aaccactggt
     1321 acgtctcgac cgtgacgacg ccggcgccca catccggcac gcggtcgttc ttcaacagcg
     1381 caggcgtgac gcgcttcttg tagccgcggt cgaacagctc gccgtggaac tggtccacga
     1441 ggttgcggcg gtgggtgagg atcaggacgc cgcccgtgcg gctcgcctcg acgaagccga
     1501 gcgcggccac ggtcttgccg gcgccggtcg cgtgctcgaa ccagaagcgg cgcgaggagc
     1561 ccggatcctc gggcgcctca ggcagttgct cgtcctcgtc gacacgctcc gccccgggat
     1621 cccagtcgag cggctcctcg tcgtcgccaa gctcctcgtc gccctcgagc tcctcctcgc
     1681 cggacggaag ctcctcgtac tggccgttgc ggccgttgcc gttccgcgcc gagacctgaa
     1741 gctcggacag gagcgcgata agggtgcccg agagggcgtc gacctggtgg gcgttcagca
     1801 cggtgccgtc agcgagatgc ggctcctcct cgttcagcag gcgctcgagg ccgagcatga
     1861 gcgagtactc ctggcgccac tcgaccgacg ggtagtcgcg gccctggtcg atctcctcga
     1921 gcgcggcgtc gagcgcgcgc cgcctgggcg agccgggcgc gagcacggcc gatgcctcct
     1981 caccctcgtg gaggaacggc tcccttgcga atttttcggc gcggtcgaga tcgtcgcgcg
     2041 ataccggcga cgatgcgacg acggaactgg tttcagccat atacggctct cgttggagac
     2101 gagtctgtaa atgcgccggc tgcggtggga ccgccgcggt ctggtggcga gtccctccgg
     2161 cactcctgcc tcggcgggga ctgccagcgg ctggatccgc gccctcccat tcagcgcggc
     2221 gtgtgcatct attaaagcac cgttaggcga tcgggtatgc ccgggggcga attcggcctt
     2281 gtgccatcct catccgcgcg atgaaccgca ggcgcgtgat cacggtgctc ggcgccgccg
     2341 cgctgatcgc cgtggtggtg atcgggctga tcagtgcggg cggctcgggg ccctcgtcat
     2401 cgccgaagag cgccgcaccg agcgccacgc aggtgtcgaa ggagctggcc ggcgcgcccg
     2461 cgccgctcgc cgcgctccac aatcaggcga accagctgct gcccggcggc gcgtcggcgt
     2521 tccagaagcg tctcgcggaa ctgcgcgggc acccggtcgt ggtgaacaag tgggcgtcct
     2581 ggtgcgggcc gtgccgggcc gagttccccc atttccagca ggcgtcggtc gagcacggca
     2641 agcaggtcgc cttcatcggc gtcgactcga gcgacaacaa cggtcaggcc caggctttcc
     2701 tgaagaagtt cccggtgagc tatccgagct acgaggacgg cgacctcaag atcgcgcaga
     2761 agctgaacgc ggtcggcgcc ttccccacca ccgtctacta cgactcgaag gggcggcgcg
     2821 tgggcgagca catcggcgtg taccagaagg cctcggacct cgagtccgac atcaagcgct
     2881 acgcgcagta gcgaggcggg tacgctccgc gcgctttgcc cgagctcagg atcgatccgc
     2941 tcagcggact gaggacgatc gtcgccggag agcgcgcttc gcgcccgggc ggcggcttcc
     3001 acgccgaccc gcagccgccg ctcgatccgg agagcgatcc gttcctcgag ggacacgaag
     3061 accgcacgcc gccggaggtc ttcgcgctgc ggccgaacgg aggcgcgccc aatacgccgg
     3121 gctgggtcgt acgcgtggtg ccgaacctct accccgcgct gagcgaggat gcttcggctc
     3181 gcgcgaccgc cgacccgctc gagctgggac gcggcgagcc cgaactgttc gcgacctcgc
     3241 ccgcacacgg cgcccacgag gtgatcgtga acgccccgga cccggtgacg tcgctgcgcg
     3301 cgctcgcacc cgagcaggtg gagaccgcga tgggcgtgtg gcgtgagcgc atccgcgtgc
     3361 attccgacgc ctccgtggtt cacgtgctcg tgaacgaagg ccgcgaggcc ggtgcgtccc
     3421 tgccgcacac ccacgcgcag ctctacgccc tgccattcgt gcccgcgcag gtg
//
LOCUS       DASVXS010000094         7683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_224633, whole genome shotgun sequence.
ACCESSION   DASVXS010000094 DASVXS010000000
VERSION     DASVXS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224633"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF752_05355"
     CDS             complement(<1..262)
                     /locus_tag="VF752_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855008.1"
                     /translation="MSSRRRPALAGSPVLIGAVTVLVTVVAVFLAYNANNGLPFVPTY
                     QLKAQIPNGQKLVAGNEIRLGGFLVGSIKEIKPQVVTKNGKTR"
     gene            complement(259..1803)
                     /locus_tag="VF752_05360"
     CDS             complement(259..1803)
                     /locus_tag="VF752_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855009.1"
                     /translation="MRRILAIALVVIGVPLVLVFALGANGGSGGSYQVRAVFDYVRAV
                     PGEDVKVAGARVGAIKSLDLTKDEKAAVVLNIDKPGFTPFHKDAHCTVRPQSLIGETF
                     VECTTGTTASPELDRIPEDQPGAGQHLLPVTNTSSPVDIDLLNNTLRGPYGQRLAIII
                     DEFGAGLAGRGADLNEVIHRANPALRETDKVLATLANQNRLLARLATESDRALTPLAR
                     ERTRFANFIVEANRTAQASAERRADLAATFQKLPQFLREFQPTLRDLGAFAGQASPVV
                     SDLRQAAPGLNRFLQALGPFSTAALPAFQTLGDASDTGRVALKAAQPTITKLANFASD
                     TEPVAKNLSALASSLDKTDAVSNLMKFVFFGTTSINGFDEISHFLRAGLIVNTCATYA
                     ATAPVTGCSSTFTPNKVRSAGGGSPDPILAKTRTALRDALLGKDRGKDAQSNTSAGSG
                     QSPMDIVRGLLGMKAPTTTTTPDGSKQRDKVVSGIRTGADSTPKALDTGDPAQPVLDY
                     LLGNDR"
     gene            complement(1800..3110)
                     /locus_tag="VF752_05365"
     CDS             complement(1800..3110)
                     /locus_tag="VF752_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855010.1"
                     /translation="MILAVVLVAVLMFNSGSSYQLTAYFQNAGQLVKGNQVKVGGRTV
                     GKITEVTLTNDARAKVTMNLDSDFAPVHQGTTATIRASSLSGIANRYVSLQPGPNNAP
                     KIPDDGQLSLAQTTSVVDVDQIFNTLDPKTRLGLQNIIRGSADQYRGKGREANQSLKY
                     FAPAISTTAQLTGELARDQNELRAFVRDTSGVVTTIAQRRDDLASLVRNAGQFTGAIG
                     DENQALARALDLLAPTLRQGSTTFRGLRSTLDALDPLVAASKPATKQLPEFFRALRPL
                     VRESTPTIHDLSTLVRSSGANNDLVELTQKLPKLSDEASTAFPHSISALKQSQPVLEY
                     IRPYTPDFTGWLQKFAEVAANYDANGHYARIQPVFGSFGYLNGSATPTITALSPSQRL
                     NAFSFGHQRRCPGVATQNSDGSAPFLDMGQLASSCDPGNQLPAP"
     gene            complement(3341..4285)
                     /locus_tag="VF752_05370"
     CDS             complement(3341..4285)
                     /locus_tag="VF752_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855011.1"
                     /translation="MADENRTEEPEGGRAQDASEQPTRTFDRDEDDGRAAVTHEPAPV
                     ARGEEDPYQWHTGVKRPHGADDAIEFIDVKKAFGRNAVLNGLNLGIPEGKISMILGPS
                     GTGKSVCIKHMVGLLYPDEGDVLVHGESVPNMADDQLFEMRKKFGVLFQDGALFGSMN
                     LYDNVAFPLRQHTDKGEDEIAEIVNRRLKEVGLAGAGDRMPNELSGGMRKRAGFARAL
                     VLDPQIVLFDEPDSGLDPVRTALLCELIKEIHDENGGAYVVITHDIMSARRVAEYIAI
                     LWKGRIVESGPAEELFNSDNQFVRQFLSGEAQGPLGME"
     gene            complement(4312..5112)
                     /locus_tag="VF752_05375"
     CDS             complement(4312..5112)
                     /locus_tag="VF752_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855012.1"
                     /translation="MGRTWLAVPRDWIASFGDIAKFAARDFGQIFSGRVFRFFGEALR
                     QAGILILGSSLVIWSLAFILGLQCGIEGAYFNRSVGAPAYAGVFSAWCDLREIMPYAF
                     GYMMAAKVGTGLVAEIGAMRITDEIDALEVMGISSMTFLCATRLLAAFMVLPFMYIAA
                     VGAGFFASYLAVVKQIGEVSSGGFFLIFWMFQNPPDLLYSVIKGMAMATVIVIVGTYY
                     GYTASGGPVGVGTATAKSMVLNTVMVHLVGMLGTQIFWGANPRAPIGG"
     gene            complement(5112..5918)
                     /locus_tag="VF752_05380"
     CDS             complement(5112..5918)
                     /locus_tag="VF752_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855013.1"
                     /translation="MVRAVADRAFLPTKNLLEQVGDMMILTGKTILSAIRPPYPYGTE
                     FVNQFLFALRLCWFPLLISTIAFGYGAPGLQAGNFLVLFGAIDRLGGFFVLATIREFA
                     PFVTAIILAGVAGTAITADLGARKIREELDALQVLGVDPVKNLVVPRFLALMIVTALF
                     NIYALLFGIFGGVLATLTFKAPLGPFFSTLLTNASVTDLWGSVVKTTLFGAIIAIVCC
                     YKGMTASGGAEGVGRAVNQAVVISFLGIFAFNYVFTQTLLATHPEISVIK"
     gene            6101..6691
                     /locus_tag="VF752_05385"
     CDS             6101..6691
                     /locus_tag="VF752_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855014.1"
                     /translation="MEAWGVYGGRLAAAVAAPLALLAFAGCGGGERQDANEPSGDFKV
                     QVVKADFPAKQALAQRSDMVISVKNVDTKTVPNVAVTVKSFEYNIQDSSVADPSRPIF
                     VVNQPPRGGDTAYVDTYALGALKPGQVASFHWSVTAVRPGPFKITYRVAAGLNGKARA
                     VLSEGGAPGGTFTGNVSQKAPQSKVADDGKTVVTTP"
     gene            complement(6678..6857)
                     /locus_tag="VF752_05390"
     CDS             complement(6678..6857)
                     /locus_tag="VF752_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855015.1"
                     /translation="MRRPPAGLCDSCVHQRLIRTGRGSEFSMCERSKSDPRFPKYPRV
                     PVERCPGYERRPTES"
     gene            complement(6859..>7683)
                     /locus_tag="VF752_05395"
     CDS             complement(6859..>7683)
                     /locus_tag="VF752_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8855016.1"
                     /translation="DIVPIGAIGVRSPVDDAIDRAVAAGVVVVAAAGNSGLPVCEQPS
                     AAKVLCVGAVDKRGARSFYSSFGGGLAISAPGGSSLPMAGEDILSTWNDGGYQELAGT
                     SQATPHVSGVAALLVSLGVPGQAAVNRILATAHDAGSPGPDAEYGAGIVDAAAAVSGL
                     GGGAAGGSTGGGGSGSGARISVRRVLSIRSVLRHGIPVRCTAPGSGRCAVSARAHGKK
                     IARGSRRVALGRPALVRARVTRAGKETLARVRSRVRVTIKVIFSGGSAARRITLKR"
BASE COUNT         1203 a         2573 c         2700 g         1207 t
ORIGIN      
        1 cgcgcgtctt gccgttcttg gtcacgacct ggggcttgat ctccttgatg gagcccacga
       61 ggaagccccc gagccggatc tcgttgcccg ccaccagctt ctgtccgttc gggatctgcg
      121 ccttgagctg ataggtcggc acgaacggca ggccgttgtt cgcgttgtag gcgaggaaca
      181 ccgccaccac ggtcaccagc accgtcaccg cgccgatcag gaccgggctg cccgcgagtg
      241 ccggtctgcg ccgcgagctc atcggtcgtt ccccagcagg tagtcgagca cgggctgcgc
      301 cgggtcaccg gtgtcgagcg ccttgggcgt ggagtcagcg cccgtgcgaa tcccggagac
      361 gaccttgtcg cgctgcttgg atccgtcagg cgtggtggtg gtcgtgggcg ccttcatgcc
      421 gagcaggccg cgcacgatgt ccatgggcga ctgccccgag ccggcgctcg tgttcgactg
      481 tgcgtccttg ccccggtcct tgccgagcaa cgcgtcgcgc agtgccgtgc gcgtcttggc
      541 gaggatcggg tcgggcgagc cgccgccggc ggaccgcacc ttgttcggcg tgaaggtgga
      601 cgagcacccg gtgacgggcg ccgtcgccgc gtacgtggcg cacgtgttca cgatcagccc
      661 ggcgcgcagg aagtggctga tctcgtcgaa gccgttgatc gacgtggtgc cgaagaagac
      721 gaacttcatg aggttgctga ccgcgtcggt cttgtcgagg ctcgacgcga gcgccgagag
      781 gttcttcgcc accggctcgg tgtccgaggc gaagttcgcc agcttggtga tcgtcggctg
      841 cgccgccttc agcgccacgc ggccggtgtc gctcgcgtcg ccgagcgtct ggaaggcggg
      901 caaggccgcg gtcgagaacg gcccgagcgc ctggaggaag cggttcaggc ccggcgccgc
      961 ctggcgcagg tcagacacca cgggcgacgc ctggccggcg aacgcgccca ggtcccggag
     1021 cgtgggttgg aactcgcgca ggaactgcgg cagcttctgg aaggtcgcgg cgaggtcggc
     1081 ccggcgctcg gccgacgcct gcgcggtgcg gttggcctcg acgatgaagt tcgcgaagcg
     1141 cgtccgctcg cgcgcgagcg gggtgagcgc gcggtccgac tcggtggcca gccgcgcaag
     1201 caggcggttc tggttggcga gagtggcgag caccttgtcg gtctcgcgga gcgcgggatt
     1261 cgcacggtgg atgacctcgt tcaggtcggc gccgcggccg gcgagacccg cgccgaactc
     1321 gtcgatgatg atcgcgagcc gctgcccgta ggggccgcgg agcgtgttgt tcaggaggtc
     1381 gatgtcgacc ggcgagctcg tgttcgtgac cggcaacagg tgctggcccg cgccgggctg
     1441 gtcctcgggg atgcggtcga gctccggcga ggcagtcgtg ccggtggtgc actcgacgaa
     1501 cgtctcgccg atcaatgact gcggcctgac cgtgcagtgg gcgtccttgt ggaagggggt
     1561 gaagccgggc ttgtcgatgt tcaacacgac cgcggccttc tcgtccttcg tcaggtcgag
     1621 cgacttgatc gcgcccacgc gcgcacccgc gaccttcacg tcctcgccag gcaccgcgcg
     1681 cacgtagtcg aacaccgcgc gcacctggta gctcccgccg ctgccgccgt tcgcgccgag
     1741 ggcgaacacg agcaccagcg gcactccgat caccacgagg gctatcgcga ggatccgtct
     1801 catggcgcgg gcagttggtt gccggggtcg caggacgagg cgagctggcc catgtcgagg
     1861 aacggcgccg agccgtcgct gttctgcgtg gcaacgcccg ggcaccgccg ctggtggccg
     1921 aacgagaagg cgttcaggcg ctgggacggc gacagcgcgg tgatcgtcgg cgtggctgag
     1981 ccgttcaggt agccgaacga gccgaacacc ggctggatgc gggcgtagtg gccgttggcg
     2041 tcgtagttcg ccgcgacctc ggcaaacttc tggagccagc cggtgaagtc gggcgtgtac
     2101 gggcggatgt actcgagcac gggctgcgac tgcttcagcg ccgagatcga gtgcgggaac
     2161 gcggtgctcg cctcgtcgga gagcttcggc agcttctgcg tcagctcgac gaggtcgttg
     2221 ttcgcgcccg aggagcgcac gagcgtgctc aggtcatgga tggtcggcgt cgactcgcgc
     2281 accagcggcc gcagcgcgcg gaagaactcc ggcagctgct tggtggctgg cttcgacgcg
     2341 gccacgagcg ggtcgagcgc gtcgagcgtc gagcgcaggc cgcggaaggt ggtcgagccc
     2401 tggcgcagcg tcggcgcgag caggtcgagc gcgcgcgcga gcgcctgatt ctcgtcgccg
     2461 atcgcgccgg tgaactggcc ggcgttccgc accaggctgg cgaggtcgtc gcggcgctgg
     2521 gcgatcgtcg tgacgacgcc ggaggtgtcg cgcacgaatg cgcgcagctc gttctggtcg
     2581 cgagcgagct cgccggtgag ctgcgcggtc gtcgagatcg cgggcgcgaa gtacttgagg
     2641 ctctggttgg cctcgcggcc cttgccgcgg tactggtcgg ccgagccgcg gatgatgttc
     2701 tggaggccaa gccgcgtctt cgggtcgagc gtgttgaaga tctggtcgac gtccacgacc
     2761 gacgttgtct gcgcgagcga cagctgcccg tcgtcgggga tcttcggcgc gttgttaggg
     2821 cccggttgga gcgacacgta gcggttcgcg atgccggaca gcgagctcgc gcggatcgtc
     2881 gccgtcgtgc cctggtgcac gggcgcgaag tccgagtcga ggttcatcgt caccttcgcg
     2941 cgcgcgtcgt tggtgagggt gacttcggtg atcttgccga ccgtccgccc gccgaccttc
     3001 acctgattgc ccttcacgag ctggcccgcg ttctggaagt aggcggtcag ctggtagctc
     3061 gagccgctgt tgaacatgag cacggcgacg agcaccaccg cgaggatcac ggcggcgatg
     3121 gcgaggaccc gggcgaagcc cggccttgcg cccctcgagg ggcgtcctag actgccgccg
     3181 gcctgtacag gtcctggggt agctggcgag cccaagtgcg agtccaatca caccctggcg
     3241 atgtccggtc gccaaggcaa tcgagccaaa tcacgtccgg ggcggcctgc cccattgggt
     3301 actgacgatc cacgagcgga ttaatcggcc gcggtgagcc ttactccatg cccagagggc
     3361 cctgggcttc gcccgacagg aactgccgca cgaactggtt gtccgagttg aacagctcct
     3421 ccgcggggcc tgactccacg atcctgccct tccagaggat cgcgatgtac tcggcgaccc
     3481 tgcgcgcgga catgatgtcg tgggtgatca cgacgtatgc cccgccgttc tcatcgtgga
     3541 tctccttgat gagctcgcag agcagcgcgg tgcggacggg gtcgaggccg gagtccggct
     3601 cgtcgaacag aacgatctgc gggtcgagga ccagagcgcg tgcgaagcct gctcgcttgc
     3661 gcattccacc cgacagctcg ttgggcatgc gatcgccggc accggcgagg cctacctcct
     3721 tgaggcgccg gttgacgatc tccgcgatct cgtcctcgcc cttgtccgtg tgctggcgaa
     3781 gcgggaacgc gacgttgtcg tagaggttca tggagccgaa gagcgcgccg tcctggaaca
     3841 ggacgccgaa cttcttgcgc atctcgaaca gctggtcgtc ggccatgttc ggcaccgact
     3901 cgccgtgcac gagcacgtcg ccctcgtccg ggtagagcag gccgaccatg tgcttgatgc
     3961 agacgctctt gccggtgccc gagggcccca ggatcatcga gatcttgccc tccgggatgc
     4021 cgaggttgag gccgttgaga accgcgttgc ggccgaaggc cttcttcacg tcgatgaact
     4081 cgatcgcgtc gtcggcgcca tgcgggcgct tcacgccggt gtgccactgg taggggtctt
     4141 cctcgccgcg cgctaccggc gccggctcgt gtgtcaccgc cgcgcggccg tcgtcctcat
     4201 cgcggtcgaa cgtgcgcgtc ggctgctcgg acgcgtcctg cgcccgaccc ccctccggct
     4261 cttccgtgcg gttctcgtca gccacttctc tccatccctt ctaggcggcg tctagccgcc
     4321 gattggtgct ctcggattgg caccccagaa aatttgcgta ccaagcatcc ctacgagatg
     4381 caccatcacg gtattcagca ccatagattt cgccgtcgct gtgcccacgc ccacagggcc
     4441 accgctggcc gtgtagccgt agtacgtgcc cacgatgacg atgaccgtcg ccatcgccat
     4501 gcccttgatc acgctgtata agaggtcggg cgggttctgg aacatccaga agatcaggaa
     4561 gaagccgccg gacgagacct cgccgatctg cttcaccacc gcgaggtaag aggcgaagaa
     4621 gcccgcgccc accgccgcga tgtacatgaa cggcagcacc atgaatgccg ccagcagtcg
     4681 ggtggcgcag aggaaggtca tcgagctgat gcccatcacc tcgagcgcgt cgatctcgtc
     4741 ggtgatgcgc atggcgccga tctcggccac gaggccggtg cccaccttcg cggccatcat
     4801 gtagccgaac gcgtagggca tgatctcgcg caggtcgcac cacgccgaga acacgccggc
     4861 gtacgcggga gcgcccaccg agcggttgaa gtaggcgccc tcgatcccgc actggaggcc
     4921 aaggatgaac gccaggctcc agatcacgag cgacgagccg aggatcagga tgcccgcttg
     4981 gcggagcgcc tcgccgaaga agcggaacac gcgcccgctg aagatctgcc cgaagtcgcg
     5041 cgcggcgaac ttggcgatgt cgccgaacga tgcgatccag tcgcgcggga ctgccagcca
     5101 ggtgcgtccc attacttgat caccgagatc tcgggatggg ttgcgagcaa cgtctgtgtg
     5161 aacacgtagt tgaacgcgaa gatgcccagg aacgagatca cgaccgcctg gttcaccgcg
     5221 cggccgacgc cctcggcgcc gccggaggcg gtcatcccct tgtaacagca gacgatcgcg
     5281 atgatcgcgc cgaacagcgt ggtcttcacc accgagcccc agaggtccgt gaccgaagcg
     5341 ttcgtgagca gcgtcgagaa gaacggcccg agcggtgcct tgaacgtgag cgtcgcgagc
     5401 acgccgccga agatgccgaa cagcagcgcg tagatgttga agagcgcggt gacgatcatc
     5461 agcgcgagga agcgcggcac aacgaggttt ttgaccgggt cgacgcccag aacctggagg
     5521 gcgtcgagct cctcgcggat cttgcgcgcg ccgaggtccg ccgtgatggc ggtgccggcc
     5581 acgccggcga ggatgatcgc ggtcacgaac ggcgcgaact cacggatcgt ggcgagcacg
     5641 aagaagccgc cgaggcggtc gatcgcgccg aacaggacga ggaagttgcc cgcctgaagg
     5701 ccgggcgcgc cgtacccgaa cgcgatcgtg gagatcagca gggggaacca gcagagcctg
     5761 agcgcgaaca ggaactggtt cacgaactcc gtgccgtatg ggtacggagg acggatcgcc
     5821 gacaagatcg tcttgccggt cagaatcatc atgtcgccga cctgctcgag caggttcttt
     5881 gtcggtagga acgctcgatc cgctacagca cgaaccacta agaaactcct cccagagcgc
     5941 cgtcccccac ggcgcactcc atcgcgcgtg ggaagcctac ggcatcgctc agggtcccac
     6001 aaccgacaac cgttcgcacg ttctgcccgg ttatggacgg gttatgccgc gtcccgaatc
     6061 ggcgcccggc gcgcgtttcc ggtggtacat tcagccgcgc gtggaagcct ggggagtgta
     6121 cggagggagg ctggctgcgg cggtcgctgc acccctcgcg ctgctcgcgt tcgcgggctg
     6181 cggcggcggt gagcgccaag acgcgaacga gccgtcaggc gacttcaagg tccaggtcgt
     6241 gaaggccgac ttcccggcga agcaggcgct cgcgcagcgc agcgacatgg tgatcagcgt
     6301 gaagaacgtc gacaccaaga cggtcccgaa cgtcgcggtc accgtgaagt cgttcgagta
     6361 caacattcag gacagctcgg tcgccgatcc gtcgcggccg atcttcgtgg tcaaccagcc
     6421 gccgcgcggc ggggacaccg cctacgtcga cacctacgcg ctcggcgcgc tcaagccggg
     6481 gcaggtcgcg agcttccact ggagcgtcac ggccgtgcga cccggcccgt tcaagatcac
     6541 ctaccgcgtc gcggccggtc tgaacggcaa ggcacgcgca gtgctgtcgg agggcggcgc
     6601 gcccggcggc acgttcaccg ggaacgtctc gcagaaggcg ccgcagagca aggtcgccga
     6661 cgacggcaag accgtcgtca cgactccgta ggccggcgct cgtagccggg acagcgctcg
     6721 accggcacgc gcgggtactt cgggaagcgc gggtcgctct tcgagcgctc gcacatgctg
     6781 aactccgacc cgcgcccggt gcggatcagg cgctggtgga cgcaggagtc acagagtccg
     6841 gccggtgggc gtcgcacgtc agcgcttcag cgtaatcctc cgggccgcgc ttccgccgga
     6901 gaaaatcacc tttattgtca cacgcacgcg cgaacgcacg cgggcgagcg tttctttccc
     6961 cgcacgcgtc acacgcgcgc gcacgagcgc cggccgcccg agtgccaccc gccgcgatcc
     7021 gcgcgcgatc ttcttcccgt gcgcgcgggc gctcaccgcg cagcggccgc ttccgggcgc
     7081 cgtgcagcgc accgggatgc cgtggcgcaa cacgctgcgg atgctgagca cgcgccgcac
     7141 ggagatgcgt gcgcccgagc ccgagccgcc gccgcccgtg gagcctccgg cggcgccgcc
     7201 gcccagcccg ctcaccgctg ccgcggcgtc gacgattccc gccccgtact cggcgtcagg
     7261 tccgggagag ccggcgtcgt gggccgtcgc gaggatgcgg ttcacggccg cctgacccgg
     7321 gacgccgagc gacacgagca gggcagcaac gcccgacacg tgcggcgtcg cttgcgaggt
     7381 gccggccagc tcctggtagc cgccgtcgtt ccaggtcgag aggatgtcct cgcccgccat
     7441 cggcaggctc gagccgcccg gcgcgctgat cgcgaggccg ccgccgaagc tcgagtagaa
     7501 gctccgagcg ccgcgcttgt cgacggcacc gacgcacagg accttcgccg ccgagggctg
     7561 ctcgcacacc ggcaggccgc tgttgcccgc ggccgctacg acgacgacgc ccgcagccac
     7621 cgcgcggtcg atcgcgtcgt ccacagggct gcgcacgccg atcgccccga tcggcacgat
     7681 gtc
//
LOCUS       DASVXS010000095         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1073510, whole genome shotgun sequence.
ACCESSION   DASVXS010000095 DASVXS010000000
VERSION     DASVXS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073510"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..524
                     /locus_tag="VF752_05400"
     CDS             51..524
                     /locus_tag="VF752_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855017.1"
                     /translation="MRAGASTEGGSLATKVAIIALMVLGSLSLWLGSPVFWLWLTSKL
                     QSDGTQAKMGPYALLLIGLILSSVAIAKGLASLQRLYGRVSGTTPTVRVILPWRRSLR
                     GGRSQKRETDGRLPLSVLDVVMVISVIVAVAAFLVWYVIANPTPPNVGGPGPAKH"
     gene            560..1612
                     /locus_tag="VF752_05405"
     CDS             560..1612
                     /locus_tag="VF752_05405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8855018.1"
                     /translation="MELGVYTFAELAADPATGTAISPRQRMHDLMEEIELADRLGLQV
                     FGVGEHHRPDFVVSSPAVVLGAAAAVTNNIRLTSAVTVLSSDDPVRVFEDFATIDLLS
                     DGRAEIMAGRGSFIESFPLFGYDLDDYDELFAEKLELLLKLIESERVTWEGKHRAAIE
                     DLGVYPRPEQDPLPVWIAVGGNPQSVIRAGLLGLPLAVAIIGGMPERFGPLVELYRQA
                     LTQAGHDPATPVGINSHTYVADTSEQAADEFFRPYATMMNRIGRERGWSGMNREQYEQ
                     LRGPRGALVVGSPQDVIDKILFEHEIFGNQRFLAQMSVGFLPHEKVMRSIELFATEVA
                     PAVREEVARREAAEAA"
     gene            1617..2501
                     /locus_tag="VF752_05410"
     CDS             1617..2501
                     /locus_tag="VF752_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX8855019.1"
                     /translation="MAKALDLEEQFRVAGEEFRLADQDPNHTAGLDKPRAADMRNADL
                     ESLKELQKRLYAEHRQALLVVLQGMDASGKDGAIAHVMAGVNPQGVHVTSFKRPSQVE
                     SGHDWLWRHVVALPERGDIGVFNRSHYEEVVTVRVHPEYLAPGAVHDVEPPDDAFWQR
                     RFDAITSWERHLVSSGTRVVKFFLHVSKKEQRKRLLDRASDPDKQWKFLPEDVAERAY
                     WDDYQAAYQDALRATSGDEAPWYAIPADHKWFARTAVANIIVRHLEQMDPRFPEVDEH
                     ARRTALEVLLEEDAAGGS"
     gene            complement(2498..2749)
                     /locus_tag="VF752_05415"
     CDS             complement(2498..2749)
                     /locus_tag="VF752_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855020.1"
                     /translation="MLCTALTAVSCAVLLGAAAIVPAPTAALPFAVIVCIGCPMVATL
                     EVPRAVAVLRLHRKANSRALAKLRRSLDRLPETEHPLGL"
     gene            complement(2838..>3084)
                     /locus_tag="VF752_05420"
     CDS             complement(2838..>3084)
                     /locus_tag="VF752_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8855021.1"
                     /translation="VVNLAAAEGNPASVMDISFANQALATEHIVLHGAELEPRVHEVP
                     QSVDEEIARLKLEALGVSIDELTEAQRAYLNSWELGT"
BASE COUNT          513 a         1034 c         1047 g          490 t
ORIGIN      
        1 ggatagccta acgctcacat acagcagtgt attcggatac actgacgtat gtgagggcgg
       61 gagcctcgac agaaggcggc tcgctggcca ccaaggttgc gatcatcgcg ctgatggtgc
      121 tcggcagcct gagcctgtgg ctcggcagcc cggtgttctg gctctggctc acctcgaagc
      181 tgcagtccga cggaacgcag gcgaagatgg ggccctacgc gctgcttctc atcggtctga
      241 tcctgtcgag cgtcgcgatc gccaagggcc tggcgagcct gcagcggctg tacggccggg
      301 tcagcggcac gacgccgacc gttcgcgtga tcctcccctg gcgccggagc cttcgcggcg
      361 gccgcagcca gaagcgcgag accgacggcc gcctgccgct cagcgtgctc gacgtagtga
      421 tggtgatctc ggtgattgtc gcggtcgcgg ctttcctcgt ctggtacgtg atcgcgaatc
      481 cgacgccgcc gaacgtcggc ggccctggac ctgccaagca ctgaagcccg agaacccggc
      541 gttgcaacac tgtcgggtga tggaactcgg cgtctacacg ttcgcggagc tggctgcgga
      601 tccggcaacc ggcacggcga tcagcccgcg gcagcgcatg cacgacctga tggaggagat
      661 cgagctcgct gaccgcctcg ggctccaggt gttcggtgta ggcgagcacc accggccgga
      721 cttcgtcgtg tcctcgcccg ccgtggtgct gggcgcggcg gccgccgtca ctaacaacat
      781 caggctcacg agcgccgtga ccgtgctgag ctcggacgat ccggtgcgcg tgttcgagga
      841 cttcgcgacg atcgatctgc tctcggatgg tcgcgccgag ataatggccg ggcgcggctc
      901 gttcatcgag tcgttcccgc tgttcggcta cgacctggac gactacgacg agctgttcgc
      961 cgagaagctc gagctgctct tgaagctgat cgagtccgag cgggtgacct gggagggcaa
     1021 gcaccgggcg gcgatcgaag atctcggcgt atacccgcga ccggagcagg atccgctgcc
     1081 cgtgtggatc gcggtcggcg gcaaccctca gtcggtcatc cgcgccggcc tgctcgggct
     1141 accgctcgcg gtggcgatca tcggcgggat gcccgagcgc ttcgggcccc tcgtggagct
     1201 ctaccgccag gcgctcacgc aggcaggcca cgatccggcg acgcccgtcg gcatcaactc
     1261 gcacacgtac gtggcggaca cctccgagca ggccgccgac gagttcttca ggccctacgc
     1321 cacgatgatg aatcgcatcg gccgtgagcg cggatggtcg ggcatgaacc gcgagcagta
     1381 cgagcagctc cgcgggccgc gcggtgcgct cgtcgtgggt agcccgcagg acgtgatcga
     1441 caagatcctg ttcgagcacg agatcttcgg caaccagcgc ttcctcgctc agatgagcgt
     1501 cggctttcta ccgcacgaga aggtgatgcg ctcgatcgag ctgttcgcga ccgaggtcgc
     1561 ccccgccgtg cgcgaggagg tcgcgcggcg cgaagcggcg gaagcggcgt agcgccgtgg
     1621 ccaaggccct tgacctcgag gagcagttcc gggtagccgg cgaagagttc cgcctggcgg
     1681 atcaggatcc gaaccacact gcgggactcg acaagccccg agcggcggac atgcggaacg
     1741 cggacctcga gagcctgaag gagctgcaga agcgtctcta cgccgagcac cgtcaggcac
     1801 tcctcgtggt cctgcaggga atggacgcat ctggcaagga cggtgcgatc gctcacgtga
     1861 tggcgggtgt gaacccgcag ggtgttcacg tcacgtcgtt caagcgcccc agccaggtgg
     1921 agagcggaca cgactggctc tggcgtcacg tggtggctct ccccgagcgc ggcgatatcg
     1981 gtgtcttcaa ccgctcccac tacgaggagg tcgtgacggt gcgcgtgcac cccgagtacc
     2041 tcgcccccgg ggccgtccac gatgtcgaac ctccggacga tgcgttctgg cagcgccgct
     2101 tcgacgcgat cacgtcctgg gagcggcatc tggtctcgag cggcacgcgc gtcgtcaagt
     2161 tctttctcca cgtctccaag aaggagcagc ggaaacgtct gctcgaccgc gcctccgacc
     2221 ctgacaagca gtggaagttc cttcccgagg acgtcgccga gcgggcctac tgggacgact
     2281 accaggccgc ctaccaggat gcgctgcgcg cgacgagcgg cgacgaggcg ccctggtacg
     2341 cgatccccgc ggaccacaag tggttcgcgc ggacggcggt ggccaacatc atcgtgaggc
     2401 acctcgagca gatggatccg cgcttccccg aggtggacga acacgcgcgc cggaccgcgc
     2461 tcgaggttct cctcgaagaa gatgccgccg gcggaagcta gagccccagc gggtgctcgg
     2521 tctcaggcag ccgatcaagg ctgcggcgca gcttggccag cgcccgcgag ttcgccttgc
     2581 ggtgcaggcg gaggaccgcg acggcgcgtg gcacctccag cgtcgcgacc atcggacacc
     2641 cgatgcagac gatcaccgcg aacggcaagg ctgccgtcgg cgcgggcacg atggcggcgg
     2701 cacccagcag caccgcacac gacaccgccg tcagggcagt gcagagcaag gctaagaggg
     2761 cctgccgtcg tttcccgctg gacgaccgca ttgtctgccc acattaacgc gcaagctttc
     2821 ctgactgccc cagtacctca ggtaccgagc tcccagctgt tcaggtaagc ccgctgcgcc
     2881 tcggtgagct cgtcgatcga gacaccgagc gcttcgagtt tcagccgcgc aatctcctcg
     2941 tccacactct gcggcacctc gtgcacgcgc ggctcgagct cggcgccgtg aagcacgatg
     3001 tgctcggtgg cgagcgcctg gttcgcgaag ctgatgtcca tcacggacgc cgggttgccc
     3061 tcggccgccg cgaggttcac cacg
//
LOCUS       DASVXS010000096         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_379651, whole genome shotgun sequence.
ACCESSION   DASVXS010000096 DASVXS010000000
VERSION     DASVXS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379651"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF752_05425"
     CDS             complement(<1..577)
                     /locus_tag="VF752_05425"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX8855022.1"
                     /translation="MTAHTVGTREEWLAARRELLRREKELTRLNDEVTRQRLELPWVP
                     VEKDYRFQTSEGEKTLAELFDGRPQLVVYHFMFGPDWDEGCPSCSLSADDFDGPMIHL
                     NQRGVTFVCISRAPLEKLETYKRRMGWTFEWVSSYGSDFNFDLGVSFTPEQQATQGEY
                     NFAPIEKPYEELPGMSAFALEDGVVYHTYSAF"
     gene            complement(658..1434)
                     /locus_tag="VF752_05430"
     CDS             complement(658..1434)
                     /locus_tag="VF752_05430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8855023.1"
                     /translation="MRPEQPSATARGAAAHRAAHQVLEGGAVFSDPLADALLDQTKEE
                     IAAAAREEPALEPMRLFVAKRARVAEDALARAFARGVRQAVVLGAGLDTFGLRNPHDG
                     LQVFEVDHPATQAWKRERIDSAGLEIPRTLRFAPIDFERQALGEALGAAGFHEERAAF
                     FTWLGVVPYLTREAIEATLAFIGSLENSEVVLDYGEPLDDLPAERRPLARELAERVAD
                     AGEPLTSHFKPSEMAALLERHGFTELDDSGFRLHVLHARR"
     gene            complement(1440..1841)
                     /locus_tag="VF752_05435"
     CDS             complement(1440..1841)
                     /locus_tag="VF752_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855024.1"
                     /translation="MPAGDERVIYHVGIGVRDFDESLEFYSRALKPLGIELVERREMD
                     NRAAVFGAGGQDDLLIFEGRPQARVHIAFQTTSREEVDAFHSEAIAAGGRDNGAPGVR
                     TEYAADYYAAYVLDPNGNNIEAVWRGGGDAA"
     gene            1962..2534
                     /locus_tag="VF752_05440"
     CDS             1962..2534
                     /locus_tag="VF752_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8855025.1"
                     /translation="MQVSGLEELPASGPVLLAGNHDSYWDPVAIGTAGLPRRQIRALA
                     KSSLWKVRPLAPVLDGMGQIPIERGTGDTGALDRAIAELSEGACIGVFPEGTRSLGRE
                     LRARSGFGRLARAVPEAEVVCCTVVGTTQVSRFPERPSIAVRFFRPEGGGLQDGESDA
                     DFVVRLLAEIRAQAPVTATGRKRPAPEPVA"
     gene            2731..2886
                     /locus_tag="VF752_05445"
     CDS             2731..2886
                     /locus_tag="VF752_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855026.1"
                     /translation="MFVDVVSLIAAAGGVAVMIWLVVIAFRGDPERRAEEAARAYFDE
                     HGRWPDD"
     gene            complement(2883..3092)
                     /locus_tag="VF752_05450"
     CDS             complement(2883..3092)
                     /locus_tag="VF752_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855027.1"
                     /translation="MTDRLDSLLGPGRPELSCDECFEQLDRYVEIELAGRDADAAIPG
                     MLAHLEGCPACREDHDSLVALLQAG"
     gene            complement(3089..3727)
                     /locus_tag="VF752_05455"
     CDS             complement(3089..3727)
                     /locus_tag="VF752_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8855028.1"
                     /translation="MSVQRPAVSGERSVRDDESAAWVEALSGGGPRQQEALDRLHALL
                     LRAARFEIARRRSSGDLDDIAHEAADDALVAIMGKLDSFRGDSRFTTWAYKFALLEAG
                     VKLRRRAWEGREVPLEEEGWARLSDVRSSPARSTETSELIDALTCAIRDELSPHQRSV
                     LIALTLNDVPIDVLAERLATTRGALYKTLHDARKKLRTQLAASGFETEGGSS"
     gene            complement(3741..3968)
                     /locus_tag="VF752_05460"
     CDS             complement(3741..3968)
                     /locus_tag="VF752_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855029.1"
                     /translation="MSRRKRTEVDPEPPSPVIDWRRDRLMSAGFSPELAAWLAGDCGI
                     DLHAVLELTDRGCPPELAARILAPLDGPRPC"
     gene            4067..4510
                     /locus_tag="VF752_05465"
     CDS             4067..4510
                     /locus_tag="VF752_05465"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX8855030.1"
                     /translation="MTTASTIHPQTAPRIDGTVTAAPTDWRDPRYQAFALLRVAFTVA
                     PIAFGLDKFFNVLVDWPKYLASWVNNIAPGSAQDFMYFVGGVEILAGVIVAIKPRYGA
                     YVVAAWLAGIIINLLSYSGYYDIALRDFGLMLGALTLGRLASVYD"
     gene            complement(4687..5178)
                     /locus_tag="VF752_05470"
     CDS             complement(4687..5178)
                     /locus_tag="VF752_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855031.1"
                     /translation="MSPTNHQTVRLSRGSHTSPNDGVCVMELASMLAGERFSDHPRSV
                     CPVIATVLRAYNDAIDDERRRDLYPLAADAVGTRGSRAERRQRLKMCGDFFGVRVGRL
                     PGPGGNLYTLALAVQHAATDASDSSHRSILELFQRLVGTSDRDDARPPAEEPLAVEPV
                     GQA"
     gene            complement(5286..>6311)
                     /locus_tag="VF752_05475"
     CDS             complement(5286..>6311)
                     /locus_tag="VF752_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8855032.1"
                     /translation="KSGGSAAAIAARAQQYGVNTVFVKSSDGSSSWSQFSSTFVQQLK
                     SAGVHVCAWQFVYGDHPDTEAALGARAAQMGADCLVIDAEGQYEGKYAQAQTYINSLR
                     TAVGPDYPLSLSSFPYVDYHPSLPYSVFLGPNGAQYNQPQMYWKTIGTSVDNIFSHTY
                     TWNGVYGKRIFPLGQLYSSPSTSDIKRFRGLAAGYGASGVSWWSWQSAAASGWNAIAP
                     PVPTTVSAPATGYPPLRSGSKGDVVVWAQQHLSAAGQPVQPTGVFDTATKQSVASFQT
                     ANTLPATGSIDNATWAALLRYQPEPVSWTARSGARAATAAGSRNGPRSARLRAKRNEI
                     PPASARR"
BASE COUNT          931 a         2175 c         2174 g         1031 t
ORIGIN      
        1 cgaacgccga gtacgtgtgg tacacgacgc cgtcctctag cgcgaaggcg ctcatgccgg
       61 gcagctcctc gtagggcttc tcgatcgggg cgaagttgta ctcgccctga gtcgcctgct
      121 gttcgggtgt gaacgaaacg ccgaggtcga agttgaagtc gctcccgtac gaggagaccc
      181 actcgaacgt ccagcccatc cggcgcttgt aggtctcgag cttctcgagc ggcgcgcgcg
      241 agatgcagac gaacgtcacg ccgcgctggt tgaggtggat catcgggccg tcgaagtcgt
      301 cggccgacaa cgagcagctg gggcatcctt cgtcccagtc ggggccgaac atgaagtggt
      361 agacgaccag ctgcgggcgc ccgtcgaaga gctccgcgag ggtcttctcg ccctcgctcg
      421 tctggaagcg gtagtccttc tcgaccggca cccacggcag ctcgaggcgc tgccgggtga
      481 cctcgtcgtt gagcctcgtc agctccttct cccgccggag taactcgcgc cgggctgcca
      541 gccattcctc gcgcgtgcca acggtgtggg cagtcacgat ccctcgcctc catttcagac
      601 gattcatgca gggattgcca ctaggacgcc ccggctcgcc ggaactaatc ggtccgctca
      661 gcggcgcgcg tgcaacacgt gcaggcggaa gcccgaatcg tccagttccg tgaagccgtg
      721 ccgctcgagc agcgcggcca tctcgctggg cttgaagtgg ctggtcaagg gctccccggc
      781 atcggccaca cgctcggcca actccctggc cagggggcgt cgctccgccg gcaggtcgtc
      841 gagcggctcg ccgtagtcga gcaccacctc cgagttctcc agcgagccga tgaaggccaa
      901 cgtcgcctcg atcgcctctc gcgtcagata cgggacgaca ccgagccatg tgaagaaggc
      961 ggcgcgctct tcatggaacc ctgcggcgcc aagtgcctca cccagggctt ggcgctcgaa
     1021 gtcgatcggg gcgaacctga gcgtccgcgg tatctcgagg ccggcggagt cgattcgctc
     1081 gcgcttccac gcctgcgtgg cgggatggtc gacctcgaag acctgcaggc cgtcgtgggg
     1141 attgcgcagg ccgaacgtgt cgaggccggc gccgagcacc acggcctggc gcacaccacg
     1201 tgcgaacgcc cgcgcgagcg cgtcttccgc gacgcgcgca cgcttcgcga cgaagaggcg
     1261 catcggctcg agcgctggct cctcgcgcgc ggcggccgcg atctcctcct tcgtctggtc
     1321 gagcagcgcg tcagcgagcg gatcggagaa caccgccccg ccctcgagca cctggtgcgc
     1381 cgcccggtgc gcagcggcgc cgcgggcggt ggcgctcggc tgctccggcc gcatcccggt
     1441 caggcggcgt cgccgccgcc ccgccagacc gcctcgatgt tgttgccgtt cgggtcgagc
     1501 acgtaggcgg cgtagtagtc cgccgcgtac tccgtgcgga cgccgggtgc tccgttgtcc
     1561 cggcccccgg ccgcgatcgc ctcggagtga aaggcgtcca cctcttcgcg cgaggtggtt
     1621 tggaacgcga tgtgcacccg cgcctgaggc cggccctcga agatcaggag gtcgtcctgc
     1681 ccgccggccc cgaaaacggc cgcgcggttg tccatctcgc ggcgctcgac gagttcgatc
     1741 ccgagcggct tcagcgcgcg cgaatagaac tcgaggctct cgtcgaagtc gcgcacgccg
     1801 atcccgacgt gatagatcac gcgctcgtcg cccgccggca tgcgcgagag actacttggc
     1861 gtctgcgagt atgccgcggc gtgccgcccg cagaacccat cccacccgct taccacgcag
     1921 caataaccct gtgctctccg atcatgcgct ggtggggccg tttgcaggtc tccggcctcg
     1981 aggagctgcc cgcgtccggg ccggtgctgc tggcgggcaa ccacgacagc tactgggacc
     2041 cggtcgcgat cggcacggcc ggcctgccgc gccgccagat tcgggcgctc gcgaagtcgt
     2101 cactctggaa ggtgcgaccg ctcgcgccgg tgctcgacgg gatgggccag atccccattg
     2161 agcgtggcac cggtgacacg ggcgccctcg accgcgcgat cgccgagctc agcgagggcg
     2221 cctgcatcgg cgtgttcccc gaggggaccc gctcgttagg ccgggagctg cgcgcacgaa
     2281 gcggcttcgg ccggctggct cgcgccgtgc cagaggccga ggtggtgtgt tgcacggtgg
     2341 ttgggaccac gcaggtctcg cgcttccccg agcgacccag catcgcggtg cgcttcttcc
     2401 gccccgaggg cggcgggtta caggacggcg agagcgacgc ggacttcgtt gtgcgcctgc
     2461 tggccgagat ccgcgctcag gcgccggtca cggcgaccgg ccggaagcgc ccggcgcccg
     2521 agccggtggc ctgagccggc gcggcactcg atttagcact taggggagcc ggattaaaag
     2581 gtctgcgaaa cgacctttaa gttgagcacc atataggggc tcaatatcag tctcgctcgc
     2641 ccgagggcgg catcggcctg cgctcccagc ctttattgcc gctgtttacg ctcgggccac
     2701 gcccccaccc gctgctctac cctgggccgg gtgttcgtcg acgtcgtgag cctgatcgct
     2761 gcggccggtg gcgtggccgt gatgatctgg ctcgtcgtga tcgccttccg tggcgatccg
     2821 gagcggcgcg ccgaggaggc cgcgcgcgcc tatttcgacg agcacggccg ctggccggac
     2881 gactagcccg cctgcagcaa cgccacgagg ctgtcgtgat cctcgcggca ggccgggcag
     2941 ccctcgaggt gcgcgagcat tccgggaata gcggcgtccg cgtcacgccc ggcgagctcg
     3001 atctctacgt agcggtcgag ctgctcgaag cactcgtcgc agctgagttc cggacggcct
     3061 ggcccgagta gcgaatcgag ccggtcggtc acgagctgcc tccttccgtt tcgaatcctg
     3121 acgccgcaag ctgcgtcctc agcttcttgc gcgcgtcgtg gagcgtcttg tagagcgcgc
     3181 ctctcgtggt ggcgagccgc tcggcgagca cgtcgatcgg tacatcgttg agcgtcagcg
     3241 caatcagcac cgagcgctgg tggggcgaga gctcgtcgcg gatggcgcag gtcagcgcgt
     3301 cgatcagctc gctcgtctcc gtgctccgtg ccggtgagga gcgaacgtcc gagaggcgcg
     3361 cccagccctc ctcctcgagc gggacctcgc gtccctccca tgcccgccgc cgcagcttca
     3421 cgcctgcttc cagcagcgcg aacttgtagg cccaggtggt gaaccggctg tcgccccgga
     3481 agctgtcgag cttccccatg atcgccacca gggcgtcgtc ggcggcctcg tgggcgatgt
     3541 cgtcgaggtc ccccgaactc cgccgccgcg cgatctcgaa tcgcgccgcc cgcagtagca
     3601 gcgcgtgaag ccggtcgagc gcctcctgct gacgtggccc gccgcctgac agcgcctcca
     3661 cccacgctgc cgattcgtcg tcgcgcaccg agcgctcgcc ggagactgct ggccgctgca
     3721 cgctcacgcc gcccacgatg tcagcacggg cgcgggccgt ctaggggagc gaggatgcgc
     3781 gccgcaagct caggcgggca gcctcggtcg gtcaactcga gcacggcgtg gaggtcgatg
     3841 ccgcagtcgc cggcgagcca ggcggccagt tccggggaga accccgcgga catgaggcgg
     3901 tcgcgacgcc aatcgatcac gggactcggc ggctcggggt cgacttcggt cctcttacgg
     3961 cggctcatgt ctgttttgcg tacaggcgtc tcgagttctt acgtaaggac tacctggtcg
     4021 gcgacgcaaa cgcgaataga ggcacccgac aaggaggctg ttccaaatga ccaccgcatc
     4081 caccattcat ccgcagacgg cgccgagaat cgacggcacc gttaccgcgg cgcccacgga
     4141 ctggcgtgat ccgcgctacc aggcattcgc gctgcttcgc gtcgccttca cggtcgcgcc
     4201 gatcgcattc ggcctcgaca agttcttcaa cgtgctggtc gattggccga agtatctggc
     4261 gtcgtgggtc aacaacatcg cgccgggtag cgcgcaggac ttcatgtact tcgtgggtgg
     4321 agtcgagata ctcgcgggcg tcatagtggc gatcaagcca cgctacggcg cgtacgtcgt
     4381 cgccgcttgg ctcgcgggca tcatcatcaa cctgctgagc tactccggct actacgacat
     4441 cgcgctgcgc gacttcggcc tcatgctcgg cgcgctcacg ctgggacggc tcgcgtcggt
     4501 atacgactga gacgtcaagc gaattaccgc ccgcccggca gcggggttga tctctgacat
     4561 cgcgtgctcg ccgaccccat ccggcgagca cgatcgatgc gtgcaaggcg agtcgacctc
     4621 gccgacggca gcgtgatgcc cacgcagggc agcgcgaacc ccgcgctcac gatcatggcg
     4681 ctggcatcac gcctggccga ccggctcgac agcaagcggc tcttcagcag gcggccgagc
     4741 gtcgtcgcga tctgatgtac cgaccagccg ctggaacagc tccaggatgc tgcggtgtga
     4801 gctatcactc gcgtcggttg cggcgtgctg caccgccagc gcaagggtgt acaggttccc
     4861 tccagggccg ggcagccgac cgactcttac gccgaagaag tccccgcaca tcttgagacg
     4921 ttggcgccgc tcggcgcgag agccgcgcgt gccgaccgcg tcggccgcca acgggtacag
     4981 gtcgcgccgg cgctcgtcgt cgatggcgtc gttgtaggcg cgtagcacgg tcgcgattac
     5041 cggacagacg gatcgagggt gatcgctgaa gcgctcgccg gcgagcatcg acgcgagctc
     5101 catcacgcag acgccgtcgt tcggggaggt gtgactgccg cggctgaggc ggacggtctg
     5161 atggttggtt ggagacatgg tcactccctt ctacttccgg tcacccattg cccggggcgg
     5221 tccggcgcaa acggcggcgc agtgctctgt ctggcgattg acccgccgtg aagaggcggt
     5281 gagagctagc gccgcgcgct cgcgggcggg atctcgttcc gcttggcccg gagccgggcg
     5341 gagcgaggcc cattgcgaga gcccgcggcg gtggcggcgc gcgccccgct gcgagccgtc
     5401 cacgagaccg gctcgggctg gtagcggagc agcgcggccc aggtcgcgtt gtcgatcgag
     5461 ccggttgcgg gaagcgtgtt cgcggtctgg aagctcgcga cagactgctt ggtggccgtg
     5521 tcgaacacgc cggtcggctg aaccggctgc cccgccgcgc tgaggtgctg ctgcgcccac
     5581 accacgacgt cgcccttcga gcccgagcgg agcggcgggt agccggtcgc cggggcggac
     5641 acggtcgtgg gtacaggtgg cgcgatcgcg ttccagccgg acgccgcggc cgactgccac
     5701 gaccaccagc tcacgccgga cgcgccatag ccggcggcga gcccgcggaa gcgctttatg
     5761 tcgctggtcg acggcgagct gtagagctgg ccgagcggga agatgcgctt cccgtacacg
     5821 ccgttccagg tgtacgtgtg gctgaagatg ttgtcgacgc tcgtcccgat cgtcttccag
     5881 tacatctgcg gctggttgta ctgcgcgccg ttcggcccga ggaacaccga gtacggcagc
     5941 gacgggtggt agtccacgta cgggaagctc gacagcgaca gcggatagtc gggaccgacc
     6001 gcggtgcgaa ggctgttgat gtacgtctgg gcctgcgcgt acttgccctc gtactgtccc
     6061 tctgcgtcga tcacgaggca gtcggcgccc atctgtgcag cccgcgcacc gagcgccgcc
     6121 tccgtgtcgg ggtggtcgcc gtagacgaat tgccacgcgc agacgtgcac gcccgcggac
     6181 ttcagctgct gcacgaatgt ggacgagaac tggctccacg acgagctgcc gtcagagctc
     6241 ttcacgaaca cggtgttcac gccgtactgc tgggcgcggg cggcgatcgc ggcggcgctg
     6301 ccgccgctct t
//
LOCUS       DASVXS010000097         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_167393, whole genome shotgun sequence.
ACCESSION   DASVXS010000097 DASVXS010000000
VERSION     DASVXS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167393"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VF752_05480"
     CDS             <1..1122
                     /locus_tag="VF752_05480"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX8855033.1"
                     /translation="GPQTAAGPEGLAAEARRPATPKLRSRKFGAAIPLDELDKKLLNL
                     MQGSFPIAPRPYAEVARLARITEDVVLRRVQRLIDERIIRQVTPIFDTRALGYSSMLV
                     AAKVDHENPHRAARIINSHPGVSHNYLRNHDFNLWFTIATEPESRLGLDGTLDVLTEL
                     TGAESVRQLPTLKLFKIRMDLEMEKGTRDLAKAGVAEEPRELEPIELSDLDIGAIRAT
                     QGDMPVIPEPYAPAAERLGLSQDRLLEHLESMRERKALRRVAAILFHRRAGFSANGMG
                     VWKVADDDILETGKLMASFRGISHCYERPTYPDWPYSVFTMAHGRSKEECDAILDSIA
                     EETGIEERATLYSSTEFKKIRLLYFTDEHRRWEAEHAGV"
     gene            1127..>1930
                     /locus_tag="VF752_05485"
     CDS             1127..>1930
                     /locus_tag="VF752_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8855034.1"
                     /translation="MTAGPLQQTRSEELYRKALGLIPGGVNSPVRAMRSIGRDPLFIE
                     RASGAEIVDVDGNSYIDYVCSWGPLIAGHAHPVVIGAVTEAAERGTSYGAPTPGEVEL
                     AAEVVARVPSAQMVRMTSSGTEASMSAIRVARAATGRDPVLKFAGAYHGHVDGLLAEA
                     GSGLATQAIPASPGVTAAQAADTEIVPWNDREAMERALAERPPAAILCEPYPANMGLV
                     PPDDGFLRFLREAADASGALLVFDEVISGFRVAKGGAQDREGVTPDLTIL"
BASE COUNT          316 a          673 c          668 g          273 t
ORIGIN      
        1 ggcccccaaa ccgctgcagg accggagggg ctcgcggctg aggcgcgccg gcccgccact
       61 cccaagctgc gctcgcgcaa gttcggcgca gcgatcccgc tcgacgagtt ggacaagaag
      121 ctgctcaacc tgatgcaggg cagcttcccg attgcgcccc ggccctacgc cgaggtggcc
      181 cgcctcgcga ggatcacaga ggacgtggtg cttcgccgcg tccagcggtt gatcgacgaa
      241 cgcatcatcc gccaggtcac gccgatcttc gacacgcgcg cgctgggcta cagctcgatg
      301 ctcgtggccg cgaaggtgga tcacgagaac ccgcaccggg ccgcgcgcat catcaactct
      361 caccccggcg tgtcgcacaa ctacctgcgc aaccacgact tcaacctctg gttcacgatc
      421 gcgacagagc ccgagtcgag gctcggccta gacggcacgc tcgacgtgct caccgagctg
      481 acgggcgcgg agtccgtgcg ccagctgccc acgctgaagc tcttcaagat ccgcatggac
      541 ctcgagatgg agaagggcac gcgcgacctg gcgaaggcgg gcgtggccga ggagccgcgc
      601 gagctcgagc cgatcgagct gtcggatctc gacatcggcg cgatccgggc gacccagggc
      661 gacatgccgg tgatccccga gccgtacgcg cccgccgcgg agcggctggg cctttcgcag
      721 gatcgtttgc tcgagcacct cgagtcgatg cgcgagcgca aggcgctgcg gcgagtcgcg
      781 gcgatcctct tccaccgccg agccggcttc tccgcgaacg gcatgggtgt ttggaaggtg
      841 gcggacgacg acatcctcga gacgggcaag ctgatggcct ccttccgcgg catctcgcac
      901 tgctacgagc ggcccacgta tccggactgg ccttacagcg tcttcacgat ggcgcacggg
      961 cgatcgaagg aggagtgcga cgcgatcttg gactcgatcg ccgaggagac ggggatcgag
     1021 gagcgcgcca cgctctactc ctccaccgaa ttcaagaaga ttcgcctgct ctacttcacc
     1081 gacgagcaca ggcgctggga ggcggagcac gccggcgtct gacaccgtga cggccggccc
     1141 gcttcaacag acacgctcgg aagagctcta ccgcaaggcg ctggggctga tccccggcgg
     1201 cgtgaactcg ccggtgcgcg cgatgcgttc gatcggccgc gacccgctct tcatcgagcg
     1261 ggcaagcggc gccgagatcg tcgacgtcga cggcaactcc tacatcgact acgtctgctc
     1321 gtgggggccg ctgatcgccg gccacgccca ccccgtcgtg atcggcgcgg tgaccgaggc
     1381 cgccgagcgc ggcacgagct acggcgcgcc caccccgggc gaggtggagc ttgccgccga
     1441 ggtcgtcgcg cgcgtgccga gtgcccagat ggtgcggatg acctcttcag gcaccgaagc
     1501 gtcgatgagc gcgatccggg tggcgcgggc cgccaccgga cgcgacccgg tgctcaagtt
     1561 cgcgggcgcc taccacggcc acgtggacgg cctgctcgcg gaggccggct caggtctcgc
     1621 gactcaggcg atacctgcga gcccaggcgt cacagcggcg caggccgccg acacggagat
     1681 cgtgccctgg aacgatcgag aggccatgga gcgggcgctg gcggagcgtc cgccggcggc
     1741 gatcctgtgc gagccctacc cggcgaacat ggggctcgtc ccgccggacg acggattcct
     1801 ccgctttctc cgtgaggcag ccgacgcatc gggcgcgctg ctcgtgttcg acgaggtgat
     1861 cagcggcttc agggtcgcga agggcggcgc gcaggatcgc gagggcgtga cgcccgatct
     1921 cacgatcctc
//
LOCUS       DASVXS010000098        10167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1370615, whole genome shotgun sequence.
ACCESSION   DASVXS010000098 DASVXS010000000
VERSION     DASVXS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370615"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF752_05490"
     CDS             complement(<1..248)
                     /locus_tag="VF752_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855035.1"
                     /translation="MLSKRCRSSRQVQTEHPLLSGRTYRNDFYDTSRLGRFKGSFPLE
                     YGGTDPDDGRFYDGDVGFSGGRVTFRVVIPKTRISVHY"
     gene            complement(498..974)
                     /locus_tag="VF752_05495"
     CDS             complement(498..974)
                     /locus_tag="VF752_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8855036.1"
                     /translation="MNSPTTPTTVRRSVTVDAARERAFYVFTASMATWWPGSHHIAKT
                     PMADIVLEPRSGGRWYEVDAEGRECEWGTVLEYDFPERVVLGWHLDPDFEYDPDPAMA
                     TQVEVRFIADGDSRTRVELEHRGFEVYGDSGRLMADRVSAEGGWGLLLEKFAEKFE"
     gene            complement(971..1300)
                     /locus_tag="VF752_05500"
     CDS             complement(971..1300)
                     /locus_tag="VF752_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855037.1"
                     /translation="MTSNLVHFELPVPDTQKGQEFYGRLLGWEFQDWDGYLMVGGASP
                     TGAVMEGNGGHPVVYFNTDDIDASVARVRDLGGTSDEPQDIPEVGRYTNCKDDQGTAF
                     GLFQGAA"
     gene            complement(1321..1650)
                     /locus_tag="VF752_05505"
     CDS             complement(1321..1650)
                     /locus_tag="VF752_05505"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8855038.1"
                     /translation="MVTYEQRAEGLTALADPTRRAVFERLADGPKPVVEIARGLPVSR
                     PAVSQHLKVLKEAGLVNDRPNGNRRIYEVDPDGVAALRDYFDQFWGQALAAFKKAAES
                     PLPEEET"
     gene            complement(1683..2402)
                     /locus_tag="VF752_05510"
     CDS             complement(1683..2402)
                     /locus_tag="VF752_05510"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8855039.1"
                     /translation="MEQVDGGEWVEFALYGAPGELPALPRGEAEVGGARVTVAGTEVP
                     DDWEERWKRFHRPVLVADKLYVRPPWEQPAVRPGVKEIVIDPGRAFGTGTHPTTRLCL
                     ELMLELSARGSFADLGCGSGVLSIAAAKLGFEPITAVDSDRLAVEATLQNARDNGVWL
                     DRVERHDLRRQPPPSAGTVAANLMRPLLLQVAKLMEEKPRALILSGLLDPETDEVVAA
                     FAPMRERRRLSLQGWSAVLLT"
     gene            complement(2480..3607)
                     /gene="dnaJ"
                     /locus_tag="VF752_05515"
     CDS             complement(2480..3607)
                     /gene="dnaJ"
                     /locus_tag="VF752_05515"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8855040.1"
                     /translation="MNSDPYEVLGVPRDADQAQIKKAFRKVARELHPDVNDSHDAEER
                     FKEAAAAHEILSDPERRSIYDRYGHEGLNSRGFEPDFTGFGSFADIFDAFFGGDPFGG
                     GFGRASRTGPAQGGDVAVAVDVSLEDAARGTEVDVEYDVVQRCERCHGNGAEPGTPIE
                     TCPRCGGTGQLRTVQRTAFGQLVRSVACDQCGGDGRIATQPCEQCGGRGLEAATLKLS
                     VEIPAGIADEQRIRLTGRGHAGERGGPPGDLYVLVRVLDDERFVRDGNDLVSVLHVPA
                     PLAALGTRLSVPTLDGDEEVEIAPGTQPNTVVTLKGKGMPSLRRGRRGDQRVVIDVVI
                     PRNLTERQQELLRELSESLGEDNLRDHADESLFSRVRRAFS"
     gene            complement(3604..4629)
                     /gene="hrcA"
                     /locus_tag="VF752_05520"
     CDS             complement(3604..4629)
                     /gene="hrcA"
                     /locus_tag="VF752_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX8855041.1"
                     /translation="MEITQRQTQVLRKVIEVHVAQGQPVGSRWLAEQGELPWKPSTIR
                     AELARLEELGLLQHPHTSAGRVPTDAGYRYYVDVLLESHDLPVPRQRLELGDMRREVD
                     EAMRTTSEQLSQVTNLLAIVTAPPIATTTIRHIEVLLLQPQVVMVVVITSTGGVTKRV
                     ISYGSPVDSGLVDWAASYLNEALGGMGLGARMLHQKLEEPSLSLAERAFLATLAPAFT
                     ELEDTAEDSLYVEGAARLFSEDRVQELSQINDLMRLLERRVALLRLLRGALSEPRIYL
                     RIGTENEAPELRSLSVVAANYGLPTRSLGAVSVLGPVRMDYGRAIVSVRQAAAELSRY
                     VGELYDE"
     gene            4699..6240
                     /locus_tag="VF752_05525"
     CDS             4699..6240
                     /locus_tag="VF752_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855042.1"
                     /translation="MERKWWTLIVVSVATFMLLLDITIVNVALPSIQKSLDASFSDLQ
                     WVVDAYALTLATLLLTAGSLADIFGRKRVFEIGLALFTISSLICGLSGSPLMLILARA
                     AQGIGGAVMFATSLALLAQAFRGRERGTAFGVWGAVIASAAAVGPLLGGALTDGLGWE
                     YIFFINVPIGVLAILLSVRQLEESRDPRARGVDWLGLVTFCGALFLLVFALIRANNVG
                     WGSTQTVSELIGSAVLLTAFVLVERHQRQPMFDLTLLKKPTFTGANIVAFTLSASMFA
                     MFLYIALYVQNIIGLSPFKTGLVFLPLTVTSFFAAPISGRLSGVVSPRVLFAVGMFLV
                     AVGLFLMHGVTASSGWTHLLPGFIISGAGIGFTNPTLASAAIGVVPPERSGMASGINS
                     TFRQVGVATGIAGLGAAFQAQVQSKITDLIAGTPAAGTGIAKQVATGNVKPALSQLPD
                     QARAVAGRAAREGFVSGLNHILYIGVGVALFGAICGLVLVRRSDYVAGAAHVQQPQET
                     EAVPA"
     gene            complement(6390..7238)
                     /locus_tag="VF752_05530"
     CDS             complement(6390..7238)
                     /locus_tag="VF752_05530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8855043.1"
                     /translation="MRAAASHEPASTHARAARAALEWAGAEGWNPGLDDEQRFRAADP
                     GAFFATERAGEIVATVSCALYGDAYAFIGFYIVRSDLRGRGIGSKLFDQALRRAAGRA
                     VGLDAVLAQKQSYEQRGFEFAHGNVRWRAAGGGEPPAGLAHLPSVPFDELVAFDASVF
                     GVERQRFLHAWVERPPGHALAYVTDGGLAGYGVLRSCQVGAKVGPLFADDEGVADALL
                     AGLLAAAPPGSDVFIDIPAANRRAHRLHAARGMKPSFETARMYRNGRPPEDLQRVFGV
                     TTLEFG"
     gene            complement(7243..8361)
                     /locus_tag="VF752_05535"
     CDS             complement(7243..8361)
                     /locus_tag="VF752_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8855044.1"
                     /translation="MTDAPASILVVDDDPINRMLLSRELEAQGHRVATVDDGLAALEA
                     LRADRFDIVLLDVLMPRLDGYDTLARIEDDERLRNVPVIMVSALEDMESVVRCIEMGA
                     ADYLPKPFDPVLLRARINGCLTTKRLRDLERRYLEQERERVRGLFARFVPEAVVDDVL
                     AHADEDLRLGGKRRMATVLISDLRGFTPFAESRPPEVVIEALNRYLSGMSEVILGRGG
                     TLTCFMGDGIMAAFGVPIEQPDHADRALEAAREMAGGALQAFNAWLRDEGIGEAFRMG
                     VGLSSGPVMAGNVGSERRLEYTAIGDTTNTAARLEAMTKETPHTVLMSDSTRSMLTRE
                     VSDLVHVDTRRVRGRRAPVHVWSVESMSPAVASSARQS"
     gene            complement(8358..>10167)
                     /locus_tag="VF752_05540"
     CDS             complement(8358..>10167)
                     /locus_tag="VF752_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8855045.1"
                     /translation="DTELTPEQRDLADVVARSGESLLGVINEILDFSKIEAGRLELEQ
                     RPLVLRDCIETALEMVAVTATGKGLEVACLVDPGAPAAIIGDSARLRQILVNLLTNAV
                     KFTEQGEVVLAVESEPEADKAGDVHTLHFSVRDTGIGIPADRIDRLFQSFSQVDASTT
                     RRYGGTGLGLAIGKRLCEAMGGEMWAESEPGKGSVFHFTVVAEATPGPTSQPVTAALR
                     GKRLLVVDDNAVNREVVTRQARSWGMVPRETGSPAQALGWIRRGDPLDVVILDMQMPD
                     MDGVMLAREIRRLRDADALPLVLLTSLGRRSDDREQGVWFAACLTKPIKASQLYSAVI
                     TAVGAGQEDAQQPAECEPDRRERAGPQLRVLVAEDNAVNQQLALKLLGRLGYVAEVAA
                     NGVEALEALRRQPFDVVLMDVEMPEMDGLEASRRIHQEWPGDERPRIIAMTANAMEGD
                     RETCLAAGMDDYISKPIHLDGLAAALGACAPESAQPERVLDPDALERLRAGAGDPAFV
                     AELVDMFASDAPALLDTLHHALEEADAEEVRRSAHTLKSNARVFGATRLAEVCQAIEN
                     LARAETLQGVDELLGQVDGEYARVEHALLAAARGQS"
BASE COUNT         1440 a         3654 c         3425 g         1648 t
ORIGIN      
        1 ttgtagtgga cggagatccg cgtcttcggg atcaccaccc ggaacgtgac cctgccgcca
       61 ctgaacccca cgtcgccgtc gtagaacctc ccgtcgtccg ggtcagtgcc gccgtactcg
      121 agggggaacg agcccttgaa tcgcccgagg cgcgaagtgt cgtagaagtc gttgcggtag
      181 gtccggcccg agagcagcgg gtgctcggtc tgcacctgtc gcgagctgcg gcacctcttg
      241 gacagcacgc tgctcgcgcc tctgattcgg ccggacaccg tgacctcgat ggaacgtccc
      301 acgtcgtccg ccggcgtcgt gttccgcgcc gatgcctcga ggctgttgag cgcggcggct
      361 ctcgcccgct cgccggccgc ggtcgaggtc ccggctggga atacgccggc gcgagcgccg
      421 ccgcgagaag cagctttgtc atgctcgact ctcgcgacga cgctataccg gcgccggacg
      481 ccgggcaagc ccgagcgcta ctcgaacttc tcggcgaact tctcgagcag taggccccag
      541 ccgccctcgg cggacacccg gtccgccatc agccgtccgc tgtcgccgta cacctcgaag
      601 ccccggtgct cgagctcgac gcgcgtgcgc gagtcgccgt cggcgatgaa gcgcacctcc
      661 acctgggtcg ccatcgccgg atcggggtcg tactcgaagt ccgggtcgag gtgccagccg
      721 agcaccacgc gctcggggaa gtcgtactcg agcacggtcc cccactcgca ctcgcggccc
      781 tcggcgtcga cctcgtacca gcgaccgcct gaacgtggct cgagcacgat gtccgccatg
      841 ggcgtcttcg cgatgtggtg actgcccggc caccaagtgg ccatcgatgc ggtgaacacg
      901 tagaacgccc gctcgcgcgc agcgtccaca gtcacgctcc ggcgaaccgt ggttggggtc
      961 gtgggcgagt tcatgccgcc ccctggaaca gtccgaacgc cgttccctgg tcgtccttgc
     1021 agttcgtgta tcggcccacc tcggggatgt cctgcggctc gtcgctcgtg ccgccgagat
     1081 cgcgcacgcg cgcgaccgag gcgtcgatgt cgtcggtgtt gaagtagacc acgggatggc
     1141 ccccgtttcc ttccatcacc gcgccggtgg ggctcgcccc tccgaccatc aggtagccgt
     1201 cccagtcctg gaactcccag ccgagcaggc ggccgtagaa ctcctgtccc ttctgcgtgt
     1261 ccggaaccgg caactcgaag tgaacgaggt tgctcgtcat cattgctcct tggttgaggt
     1321 tcaagtctcc tcctcgggga gaggggactc agcggccttc ttgaacgcgg cgagcgcctg
     1381 gccccagaac tggtcgaagt agtcgcgcag ggcggccacg ccgtctgggt cgacctcgta
     1441 gatgcggcga ttgccgttcg gccggtcgtt caccaggccc gcctccttca gcaccttcag
     1501 atgctgcgac acggcgggcc ggctcaccgg cagcccgcgg gcgatctcca ccacgggctt
     1561 cggcccgtcg gcgagccgct cgaacaccgc tcgccgcgtg gggtccgcga gtgcagtgag
     1621 accctccgct cgttgttcgt aagtcaccac ttaccgtaag tgtacgctta cggtagctcc
     1681 tgtcaagtga ggagcaccgc gctccagccc tgaagggaga ggcgtcgccg ttcgcgcatc
     1741 ggggcgaacg ccgcgacaac ctcgtccgtc tccgggtcga gcaggcccga gaggatcagc
     1801 gcgcgcggct tctcctccat aagcttcgcc acctgcagga ggagcgggcg catcaggttc
     1861 gcggcgacgg tgcccgcgga cggaggcggc tgccggcgca ggtcgtgccg ctccacgcgg
     1921 tcgagccaca cgccgttgtc gcgcgcgttc tgcagcgtcg cctcaaccgc caggcggtcg
     1981 gagtcgacgg ctgtgatcgg ctcgaagccg agcttcgcgg ccgcgatcga cagcaccccg
     2041 gaaccgcagc cgaggtcggc gaacgagccg cgcgccgaca gctccagcat caactcgagg
     2101 cacaggcggg tggtgggatg cgtgccagtc ccgaacgcgc ggccggggtc gatcacgatc
     2161 tccttcacgc ccggccgcac cgccggctgc tcccagggcg ggcgcacgta gagcttgtcg
     2221 gccacgagca ccggccggtg gaagcgcttc cagcgctcct cccagtcgtc cggcacctca
     2281 gtgcccgcga ctgtcacgcg tgcgccgccg acctcggcct cgccccgcgg cagggcgggg
     2341 agctctcccg gcgcgccgta gagcgcgaac tcgacccact caccgccgtc gacctgctcc
     2401 accccggtcg gcgcgagctc gaggagagcg gccagtacct gctccgcgga ctcggcgggc
     2461 gcgcggatcg ccaggcggat cagctgaagg cccgcctcac gcgcgagaac agcgactcgt
     2521 cggcgtggtc ccggaggttg tcctcgccaa gcgactcgct cagctcgcgc agcagctcct
     2581 gctggcgctc ggtgaggttg cgcgggatca ccacgtcgat caccacgcgc tggtccccgc
     2641 gacggccgcg ccgcagcgag ggcatgccct tgcccttgag cgtgactacg gtgttcggct
     2701 gcgtgcccgg cgcgatctcg acttcctcgt cgccgtcgag tgtcggcacg ctgagcctcg
     2761 tgccgagggc cgcgagcggc gcgggcacgt gcagcacgga cacgaggtcg ttgccgtcgc
     2821 gcacgaatcg ctcgtcgtcg agcacccgca ccagcacgta gaggtcaccg ggcggaccac
     2881 ccctctcccc ggcgtgaccg cggccggtga gccggatgcg ctgctcgtcc gcgatgccgg
     2941 ccgggatctc caccgacagc ttcagcgtgg ccgcctcgag gccgcgcccg ccgcactgct
     3001 cgcacggttg cgttgcgatg cggccatcgc cgccgcactg gtcgcaggcc actgagcgca
     3061 ccagctggcc gaaggccgtg cgctgcaccg tgcgaagctg gccggtgccg ccgcagcgcg
     3121 ggcaggtctc gattggcgtg ccgggctcgg cgccgttgcc gtggcagcgt tcgcagcgct
     3181 gcacaacgtc gtattccaca tccacctcgg tgccgcgcgc ggcgtcctcg agcgagacgt
     3241 ccacggccac cgctacgtcg ccgccctgcg cgggaccggt gcgcgacgcg cgtccgaagc
     3301 ctccgccgaa cggatccccg ccgaagaagg cgtcgaagat gtcggcgaac gagccgaagc
     3361 cggtgaagtc gggttcgaag ccgcggctgt tcaggccctc gtggccgtag cggtcgtaga
     3421 tcgagcggcg ctcggggtcg gagaggatct cgtgcgcggc ggccgcctcc ttgaagcgct
     3481 cctcggcatc gtgcgagtcg ttcacgtcgg gatgcagctc gcgagcgacc ttgcggaagg
     3541 ccttcttgat ctgcgcctga tcggcgtccc gggggacgcc caggacctca tacggatcgc
     3601 tgttcattcg tcgtaaagct cgcccacgta acgcgacagc tcagcggccg cctgcctcac
     3661 cgagacgatg gcgcggccgt agtccatgcg cacaggtccg agaacgctca cggccccaag
     3721 gctacgagtc ggcagcccgt agttcgccgc caccacgctc agcgagcgca gctccggcgc
     3781 ctcgttctcg gtgccgatgc gcagatagat gcgtggctcg ctgagggccc cccgcaggag
     3841 ccggagcagc gcgacgcgcc gctcgagcag cctcatcagg tcgttgatct gcgagagctc
     3901 ctgcacgcgg tcctcggaga acaggcgcgc ggcgccctcc acgtagaggc tgtcctccgc
     3961 ggtgtcctcg agctcggtga aggcgggcgc gagcgtggcg aggaaggcgc gctcggccaa
     4021 cgacaggctg ggctcctcga gcttctggtg cagcatgcgc gcgccgaggc ccatcccgcc
     4081 gagcgcctcg ttcagatagc tggcggccca gtccaccaag ccggagtcga cgggtgagcc
     4141 gtagctgatc actcgcttcg tgacgccgcc ggtggacgta atcacgacca ccatgacgac
     4201 ctgcggctgg agcagcagca cctcgatgtg gcggatggtg gtggtggcga tcggcggcgc
     4261 cgtcacgatc gcgagcaggt tcgtgacctg ggacagctgc tcgctcgtgg tccgcatcgc
     4321 ctcgtcgacc tcgcggcgca tgtcgccgag ctcgagccgt tgccgcggca cgggaaggtc
     4381 gtgcgactcg agcagcacgt ccacgtagta tcggtagccg gcatcggtcg ggacgcggcc
     4441 cgcggaggtg tgcgggtgct gaagcagccc gagctcctcg agcctcgcga gctcggcgcg
     4501 gatcgtggag ggcttccatg ggagctcgcc ctgctcggcg agccagcgcg atccgaccgg
     4561 ctgcccctgc gccacgtgga cctcgatcac cttccggagg acctgcgtct gtcgctgcgt
     4621 tatctccacc ccgtagattt tgaatggtcg cgtccagtcg agggcgcgcc ttccctcccc
     4681 gaccgaagga gccgcccggt ggagcgcaag tggtggacgc taatcgtcgt aagcgtcgcg
     4741 accttcatgc tgctgctcga catcacgatc gtgaatgtcg cgctgccgtc gatccagaag
     4801 tcgctcgacg ccagtttcag cgacctccag tgggtcgtcg acgcctacgc cctcacgctc
     4861 gccacgctgc tcctcacggc cggctcgctc gccgacatct tcgggcggaa gcgcgtgttc
     4921 gagatcggcc tcgcgctgtt cacgatctca tcgctgatct gcggcctctc cggctcgccg
     4981 ctgatgctga tcctcgcacg cgcggcgcag ggcatcggcg gcgcggtgat gttcgccacc
     5041 tcgctggctc tgctggcgca ggcgttccgc ggccgcgagc gcggtaccgc gttcggcgtg
     5101 tggggcgctg tgatcgcgag tgctgcggct gtcggcccgc tgctcggcgg cgcgctcacc
     5161 gacggcctcg gctgggagta catcttcttc atcaacgtgc cgattggcgt cctggcgatc
     5221 ctgctctccg tgcgccagct cgaggagtcg cgcgacccac gggcgcgcgg cgtcgactgg
     5281 ctcggcctcg tgaccttctg cggcgcactc ttcctgctcg tgttcgcgct gatccgggcg
     5341 aacaacgtgg gttggggaag cacgcagacc gtctcggagc tgatcggctc ggcggtgctg
     5401 ctcaccgcct tcgtgctcgt tgagcgccac cagcgccagc cgatgttcga cctcacgctg
     5461 ctgaagaagc cgaccttcac cggggcgaac atcgtcgcgt tcacgctgtc cgcctcgatg
     5521 ttcgcgatgt ttctctacat cgcgctctac gtgcagaaca tcatcgggct ctcgccgttc
     5581 aagaccggcc tcgtgttcct gccattgacc gtcacgtcgt tcttcgcggc gcctatctcg
     5641 gggcggctgt cgggcgtggt ttccccgcgc gttcttttcg ccgtcgggat gttcctggtc
     5701 gccgtcggcc tgttcctgat gcacggggtc acggcgtcct ctggctggac ccacttgctc
     5761 cccggcttca tcatctccgg cgcgggcatc ggcttcacca accccacgct cgcctcagcc
     5821 gcgatcggag tggtgccccc cgagcgaagc ggcatggcgt cggggatcaa ctcgaccttc
     5881 cgccaggtgg gtgtggcgac cggcatcgcg ggcctagggg ccgcgttcca ggcgcaggtg
     5941 cagtcgaaga tcaccgacct gatcgcgggc acgcccgctg cagggaccgg gatcgccaag
     6001 caggtcgcaa cgggcaacgt gaagccggcg ctctcgcagc tacccgacca ggcgcgcgcc
     6061 gtggcgggcc gggcagcgcg cgagggcttc gtcagcggcc tcaatcacat cctctacatc
     6121 ggcgtcggcg tcgccctgtt cggcgcgatc tgcgggttgg tgctggtgcg gcgctccgac
     6181 tacgttgcgg gagcggcgca cgtgcagcag ccgcaggaga cggaggcagt tccggcgtag
     6241 cgggtcgctg tcatcgcttt ttctgcgcga ccgtgaaccg cagcaatgag gaacgggccc
     6301 ggcccgggca gtcgccctga gcgtcgtcgt tttgggccga ctatgggccc aaatcgacga
     6361 cacccagcgt cggaatcagc tgccagctct cagccgaact cgagcgtcgt gacgccgaag
     6421 accctctgca ggtcctccgg cggccggccg ttgcggtaca tccgcgcggt ctcgaacgac
     6481 ggcttcatcc cgcgagcggc atgaaggcgg tgcgcgcggc gatttgccgc cggaatgtcg
     6541 ataaagacgt cgctgcctgg cggagccgcg gcaagtagcc cggcgagcag cgcatcggcc
     6601 acaccctcgt cgtccgcgaa cagcgggccc acctttgcgc ccacctggca cgagcgcaga
     6661 accccgtagc cggccaggcc gccatcagtc acataggcga gcgcgtgccc gggcggccgc
     6721 tcgacccagg cgtgcaggaa ccgttgtcgc tcgaccccga ataccgacgc gtcgaacgcc
     6781 accaattcat caaacggcac cgaggggagg tgcgccaggc cggcgggcgg ctcgccgccg
     6841 cccgcggcgc gccagcgcac attgccgtgg gcgaactcga agccgcgctg ctcgtatgac
     6901 tgtttctgcg ccagaacggc gtccagaccc actgcccgtc cggcggcgcg tcgaagcgct
     6961 tggtcgaaca gcttgctccc gatcccccgg cctcgtaaat cggaccgcac gatgtagaac
     7021 ccgatgaacg cgtacgcatc gccgtacagc gcgcaggaga cggtggcgac tatctcgccc
     7081 gcgcgctctg tcgcgaagaa cgcgcctgga tccgccgcgc gaaatcgctg ctcgtcgtcg
     7141 aggcccgggt tccagccctc ggcgcccgcc cactcgagcg cggctcgcgc tgcacgcgcg
     7201 tgtgtcgatg ccggttcgtg ggaagccgcc gcgcgcatgc ggtcaggact ggcgtgccga
     7261 ggacgccact gccgggctca tgctctcgac cgaccagacg tgcacagggg cgcggcgccc
     7321 gcgaacccgg cgcgtgtcca cgtgtacgag gtccgagacc tccctggtga gcatggaccg
     7381 cgtcgaatcg gacatgagca cggtgtgcgg cgtctccttc gtcatcgcct cgagccgcgc
     7441 cgccgtattg gtggtgtcgc cgatggccgt gtactcgaga cgccgctcgg agcccacgtt
     7501 gccggccatc accggcccgc tgctgaggcc cacccccatg cggaacgcct cgccgattcc
     7561 ctcgtccctc agccaggcgt tgaacgcctg cagcgctccg ccggccatct cgcgtgccgc
     7621 ctcgagggcc cggtcggcgt ggtcgggttg ctcgatcggg acgccgaagg cggccatgat
     7681 gccgtcgccc atgaagcagg tcagggttcc tccccgcccg aggatcacct cgctcatccc
     7741 gctcaggtag cggttgagcg cttctatcac tacctcggga ggacgactct ccgcaaaggg
     7801 ggtgaagccg cgcagatcgc tgatcagcac cgtcgccatg cggcgcttgc ctccgagccg
     7861 aagatcctcg tcggcgtgcg ccaggacgtc gtcgacgacg gcctcgggca cgaagcgcgc
     7921 gaagagcccg cggacgcgct ctcgctcctg ctcgaggtac cggcgctcca ggtcccgcag
     7981 gcgcttggtg gtgaggcagc cgttgatcct tgcgcgcagc agcaccgggt cgaagggctt
     8041 ggggaggtag tccgccgcgc ccatctcgat gcagcgcacc acgctttcca tgtcctcgag
     8101 cgccgagacc atgatcacgg gaacgttccg cagccgctcg tcgtcctcga tccgcgcgag
     8161 cgtgtcgtac ccgtccagcc gcggcatgag gacgtccaag aggacgatgt cgaaccggtc
     8221 ggctcgaagc gcctccagcg ccgccaggcc gtcgtccacc gtcgcgacgc ggtggccctg
     8281 cgcctcgagc tcacgcgaca ggagcatccg gttgatcggg tcgtcgtcca cgacgaggat
     8341 cgatgcggga gcgtcggtca tgactggccc cgcgccgccg ccaacagcgc gtgctcgacg
     8401 cgtgcgtact ccccgtcgac ctggcccaga agctcatcca cgccttgcag cgtttcggct
     8461 ctcgccaggt tttcgatcgc ttggcacacc tcggccagcc tcgtcgcccc gaacacccgc
     8521 gcgttggact tgagcgtgtg cgccgagcgg cgcacctcct ccgcgtccgc ctcctcgagc
     8581 gcgtggtgga gcgtgtcgag cagagcaggg gcatcgctgg cgaacatgtc gacgagctcg
     8641 gcaacgaagg ccgggtcgcc ggcccccgcg cggagccgct cgagtgcgtc ggggtccagg
     8701 acccgttccg gctgggccga ttcaggcgcg caggcgccaa gagccgccgc tagcccatcg
     8761 aggtggatcg gcttgctgat gtagtcgtcc atgccggccg cgaggcaggt ctcacgatcc
     8821 ccttccatcg cgttcgcggt catggcgatg atgcgcggcc gctcgtctcc aggccactcc
     8881 tggtggatcc ggcgcgaggc ctcgaggcca tccatctcgg gcatctcgac gtccatcagc
     8941 acgacgtcaa agggctgacg ccgaagcgcc tcgagcgcct ccacgccgtt cgccgcgacc
     9001 tcggccacgt atcccagccg tcccagcagc ttgagcgcga gctgctggtt taccgcgttg
     9061 tcctcggcga ccagcacgcg cagctgcggc cccgcgcgct cgcgtcgatc cggctcgcat
     9121 tcggccggct gctgggcgtc ttcctgtccg gcgccgacgg cggttatcac ggcgctgtag
     9181 agctgggagg ccttgatggg cttggtgagg cacgccgcga accagacacc ttgctcgcgg
     9241 tcgtcgcttc gccgcccgag cgaggtcagc agcacgagcg ggagagcatc cgcatcgcgg
     9301 aggcgacgga tctcgcgcgc gagcatcacg ccgtccatgt ccggcatctg catgtcgagg
     9361 atcaccacgt cgagcgggtc gccgcgacgg atccacccga gcgcctgcgc cggcgatccg
     9421 gtctcgcgcg gcaccatgcc ccacgagcgc gcctggcgcg tgaccacctc gcggttcacc
     9481 gcgttgtcgt cgacaacgag gaggcgcttg ccgcggagcg cggccgtgac cggttgtgac
     9541 gttgggccgg gggtcgcctc ggcaacgacg gtgaagtgga acaccgagcc cttgccgggc
     9601 tcgctctccg cccacatctc tccgcccatc gcctcgcaca ggcgcttgcc gatcgcaagc
     9661 ccgaggccgg tgcccccgta gcggcgcgtc gtggacgcgt ccacctggct gaacgactgg
     9721 aacaggcggt cgatccggtc cgcggggatc ccgatgcccg tgtcgcgcac gctgaagtgc
     9781 agggtgtgca catctcccgc cttgtctgcc tcgggctcgg actcaaccgc gagaaccacc
     9841 tcgccctgct cggtgaactt gaccgcgttc gtgagcaggt tgacgaggat ctggcggagg
     9901 cgtgcgctgt ccccgatgat cgccgcgggt gcgccggggt cgaccaggca cgcaacttcg
     9961 agaccctttc cggttgcggt cacggcgacc atctcgagtg ccgtctcgat gcagtcgcgc
    10021 aggacgagcg ggcgctgctc gagctcgagc ctccccgcct cgatcttcga gaagtcaagg
    10081 atctcgttga tcacgccgag cagcgattcg ccgcttcgcg cgaccacgtc ggcaaggtcg
    10141 cgctgttcgg gcgtcagctc cgtgtcg
//
LOCUS       DASVXS010000099        14321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_862133, whole genome shotgun sequence.
ACCESSION   DASVXS010000099 DASVXS010000000
VERSION     DASVXS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862133"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1025
                     /locus_tag="VF752_05545"
     CDS             168..1025
                     /locus_tag="VF752_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX8855046.1"
                     /translation="MGLLEAIASETGRAVAGGCPVGGGCINEGWRVDFEDGGRAFVKT
                     RQDVAPGEYATEAEGLRWLAEPRAVRLPEVLAVRDQQEPRFLALEWIDEGRLDAAGAE
                     ELGRGLARMHAAHADAFGGPRPLRIGPIALSNEPLADWPSFYAERRLRPLIGPARDNG
                     SLSRTGAEAVERACERMEELSGPPEPPARLHGDLWGGNVLGGADGGAWLIDPAAYAGH
                     REVDLAMLRLFGTPSARIFSAYEEEAPLYDGHEERVGLWQLFPLLVHAVLFGGHYGAS
                     VEQTARRYA"
     gene            1030..1818
                     /locus_tag="VF752_05550"
     CDS             1030..1818
                     /locus_tag="VF752_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8855047.1"
                     /translation="MDLGLAGRACIVTGASRGIGLATARMLGAEGANVLLTARGEDAL
                     REAVDEIGSGAQGLVLDVRDPAAGDRAVEECERHFGSVDVLVNNAGTSRNRPLDQLTD
                     EDWQEQWELNVMGPMRLTRAAAPKMAAAGWGRVVNVTSSAGKRPSSTNMAYAVTKAAQ
                     LSLSRSFADLYAARGVLVNAVAPGPVGTPLWMGEGGLGEQAAQARGVSLEQALEAQRA
                     KVPLGRFGTEDEIAAVIVFLCSERASYVTGAAWSVDGGTVATIL"
     gene            complement(1815..2696)
                     /locus_tag="VF752_05555"
     CDS             complement(1815..2696)
                     /locus_tag="VF752_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855048.1"
                     /translation="MRSSSSIAVVAATAAIAVGAAHVALGDPHPAVLASAGTGDVQIG
                     NSRAGQAIVTADRLAPGGTASGTVTISNAGSADGAFFLSAVDTVGTLISALQLRVDDL
                     SSAARVYDGSFAGLSQLALGNFAPGEAHAYRFTVALGAGAANALQGAEASVSFRWDAE
                     ARTAPITTEPQPAPAPEPAPAPVATSATPAPVITQLSRNAVKFSASCSSACTVKGAGS
                     VKVKGMRGRVKLTPVTKKLTAGGKASITLAVPGSARVALRKALKRGNKPVATATITAT
                     DASGRTTTVTRRITLKI"
     gene            complement(2683..3672)
                     /locus_tag="VF752_05560"
     CDS             complement(2683..3672)
                     /locus_tag="VF752_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855049.1"
                     /translation="MRTRRAVAGLALAALAGIGLGAPGAPTFSSFSATTASSGSSFSA
                     AADWTAPSASASVIGKSSGGVTGYVRPSGTYYVYANVADSGNPASGVSSVKADVSSVT
                     TGQTAVALSQGSYAAGGQTFNYRSAQLTASALPAGTKSYTPSLTDSAGNSTTQSGFSV
                     VMDSTGPSASGVQTANGGATVGKAEAGDTVTFTFNEPIEPLSILSGWSGSATAVSVRI
                     ANNTSGSTNDTLQVLASGTTTVLPLTSTAANTGLDLGGKSYVTADVTFNSSSMQVSGN
                     SVVVTLGTPSGATGTGSAGTMKWTPNAATTDRAGNVLSPVTAVSETGTSDAEF"
     gene            complement(3662..4186)
                     /locus_tag="VF752_05565"
     CDS             complement(3662..4186)
                     /locus_tag="VF752_05565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8855050.1"
                     /translation="MRRSALIALRFLAGTACGLGIGMVLAVSLPVLAGHRPFTVRSGS
                     MEPAIHTGDVVVDSRIPPSAMRVGDVVTFRDPEGSKRLITHRVRSLNTQAGRVHVVTK
                     GDANNTVERWTVPTGGEVGRVEYRVWKLGYALALVHSRTGMLALVAVPAVLLCILELV
                     SIWRPARPREEHAH"
     gene            complement(4183..4779)
                     /locus_tag="VF752_05570"
     CDS             complement(4183..4779)
                     /locus_tag="VF752_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HEX8855051.1"
                     /translation="MNLKRTRKMLRTGLALGLTAALAGGASWSAFSSTATNGGNSFAA
                     GTVSLSDNDAGQALLSLSGAKPGDSQASCLKATYAGTLPASVRLYASATGGLAPYLNL
                     VVTRGTDPAASFPSCGGFTADTTNYIGQGAGVVYSGTLAAFPSSYGAASDEPLASSPE
                     SWTQGESHSYRFTVTLANNAAAQGLTGSATFTWEARNE"
     gene            complement(4776..5285)
                     /locus_tag="VF752_05575"
     CDS             complement(4776..5285)
                     /locus_tag="VF752_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855052.1"
                     /translation="MSVRRLLRQGGLGVLAGLALAAALLCGWRVQAGAGTLGLDLTLR
                     SASSGELEVLPAAPLAHVSALRPGGPPLTGTMSVRNQTGLPLRAAFRAAPSLHDTDAA
                     LWLELRAGRRVVVRDPLSRLRGSTGGLRLRSGERRELTVRAWLPAGAASGWRGRIVDV
                     SLVPEVKPA"
     gene            complement(5293..5874)
                     /locus_tag="VF752_05580"
     CDS             complement(5293..5874)
                     /locus_tag="VF752_05580"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HEX8855053.1"
                     /translation="MSKSRKVLLSILVLGIAGSAAGFGVFSAFSSTTTNAGNSFAAGT
                     VNVGSNDAGQFLYNVSNQKPGDSVTKCIKVTYSGSLDSDVHLYTPDSVGSLGQYLDMT
                     VTPGTQPGAAFPGCTGFTPDSGATAWSGTLAQFAAAKNSYANGLSFNPGGSTKWATSD
                     SVVYRVSLSLQDTNSAKGASTGAHSFVWEARNQ"
     gene            6043..7389
                     /locus_tag="VF752_05585"
     CDS             6043..7389
                     /locus_tag="VF752_05585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8855054.1"
                     /translation="MFFPRGGSAHVNRYMARFLPRSGWDVTLVTGSLGAPGALSNAET
                     FFERLDVRPVDYTAAMSAPDPLLADPPLHPSYEERPDAPDLVFARVDDAAYEHLVAAW
                     AGALHDAGAAEADVLHLNHLTPINEAAEREFPGVPRVGHLHGTELLMLNRIDAGNPDG
                     WEHAEEWAERMRGWARRCERLLVLSPDAVERVPALLGVEPKRVVWAPNGFEPEGFERR
                     PVRGDARLAFWRRWLVEEPRGWDPESQRPGSVRYSEVDLAAFAGDSPVFLYVGRYTEV
                     KRIPLMIRAHQRALAATGAHAPLVILGGYPGEWEGQHPLEVIRETGARDVFLAGWRGH
                     DELPDGLNASDVVVLASVHEQFGQVLVEGMACGLPAIGVNAHGPATIIDPGETGWLAE
                     PDDEESLAQAMTEAIASPERRRELGERAYVAARQRYSWPALAERVARVYDEVADGR"
     gene            7422..8117
                     /locus_tag="VF752_05590"
     CDS             7422..8117
                     /locus_tag="VF752_05590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX8855055.1"
                     /translation="MSDAIPYRIRRSDRARRARIVVDAEGVEVVVPRRMALRQVEPFV
                     AEHSAWIEKTLRRYRRAEEALPRPRLEDGGWVPYMGRVLELRVRVEPGRVRDSVVRRG
                     EMALEVRVASGDEDSVRSALERWYRRRARVEVAARLDAATRRAGTSYRSLTIRSQRTR
                     WASCSSNGSMSFNWRLMLAPEAVLDYVVEHEVAHLEVLDHSPRFWRLLGSRCPDARVH
                     ERWLRKHGSALRL"
     gene            complement(8306..9040)
                     /locus_tag="VF752_05595"
     CDS             complement(8306..9040)
                     /locus_tag="VF752_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855056.1"
                     /translation="MSSAVLAWRQFRFERRMFWRNPSAAFFNFALPLLFLVLIASAFG
                     ADRKELNVLVPGLAGLAVMATTFTALAFNLTFLREQGILKRIYGTPMPTGSYFTGVIG
                     NAVANAVVQVALVVVIGHVAYHVSWPRDWGALILFTLLGVTCFGALGVAFSHAIPNFD
                     AAPAYTNAVFLPAIFISGVFYDANNLPAVLSGIAEALPLKHVIDGLSGGLVTGQGVSH
                     HLTALGVILAWTAVGLFFAIAKFRWE"
     gene            complement(9037..9936)
                     /locus_tag="VF752_05600"
     CDS             complement(9037..9936)
                     /locus_tag="VF752_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855057.1"
                     /translation="MQANAKGAQLAIVHAVNGSAIEVEDLRKSYGDHEAVRGVSFAVE
                     RGEVFGLLGPNGAGKTTTVEILEGYRKRSGGRVSVLGLDPERRDRTLRERVGIVLQSS
                     GIYPRATVREAVEHFSAMYPHPRDPAETIAVVGLEEKRDAYARDLSGGQLRRLDLALA
                     LVGDPELIFLDEPTTGFDPAARRTAWGAITALKELGKTIVLTTHYLDEAQSLADRVAI
                     IKDGVFVAEGSPGELAPSRFRYTVSYQRNGDRVEHVTDDPTTLLHELTGDALARGERL
                     EGLHVGRPTLEDVYFELTAEDAE"
     gene            9920..10798
                     /locus_tag="VF752_05605"
     CDS             9920..10798
                     /locus_tag="VF752_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855058.1"
                     /translation="MAFACIDVGSNTTRLLVADVRDGRLRELYAERVFTRIGKNLLDH
                     GEVEPAKVAEHALVVASQADTARELGAIGIDVVGTAVVRQAPNAADLVAAVDETCGLP
                     MRILEAREEARLSFAGAVATHDAPVDGTLAVLDVGGWSTEVALGTAAGGITWWESFPL
                     GSGLLADLHLRSDPPDREDVENIRAHVEEVLDGLDLSHAEGAIAVGGAAGSLRRLVGR
                     DLVPERLARCLDILLANRAADVAAHVDLHQERVRLMPAGVIVVSELVRRLAVPLELGR
                     GGLGEGVVMALARAAQ"
     gene            10897..11736
                     /locus_tag="VF752_05610"
     CDS             10897..11736
                     /locus_tag="VF752_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855059.1"
                     /translation="MGTAEHIEPTRHRRARLPRWAFAALGAAVALALVAVAVLALGGG
                     GSSASRSNAKSSNAATAKALTPEQKAALADHSADKSLKAHPQVVQVGGAGSDTGITAE
                     DQVAGISTGANAPHAPTDAEIKNELAAFKRHLATGVGLRHGPVAQVLSTGEAVAPIDA
                     PPIVDTIIRAGNAIAKTPYKWGGGHGAWSDTGYDCSGSVSFALAAAGLLNSPLDSTGL
                     SQWGAAGEGRWVTIYANGGHAFMVVAGLRFDTSGRSGPTGTRWQPAMRSTSGFVARHP
                     PGL"
     gene            complement(11733..12491)
                     /locus_tag="VF752_05615"
     CDS             complement(11733..12491)
                     /locus_tag="VF752_05615"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8855060.1"
                     /translation="MPEVTYERIGAAAVLTIDRPDRRNAVNGPTADLLGEGFHRFEAD
                     DEARALVLTGAGGVAFCAGADLKDIESLGPRLSSADGPMGFTRRTPSKPTIAAIDGWC
                     LAGGLELALWCDLRIATTTSTFGLPERRWGVPLIDGGTQRLPRVVGLGRALEMILTGR
                     MVGVEEAREIGLVNEIVDHGKALERSLELAETLAGFPQETMLADRHAAIAGLGLRLDE
                     GLALEANAGPELLRIAQQGAARFAGGEGRGGVGV"
     gene            complement(12638..12895)
                     /locus_tag="VF752_05620"
     CDS             complement(12638..12895)
                     /locus_tag="VF752_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855061.1"
                     /translation="MRRRALITLLGSAAFVLALQATAFASDDGEGLLGETDDRIVTAF
                     GLGLVIFFIAFVILASWLQGALERRKYEKRAAASRYRTSGW"
     gene            12941..13441
                     /locus_tag="VF752_05625"
     CDS             12941..13441
                     /locus_tag="VF752_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX8855062.1"
                     /translation="MPDDSLLMVVEIPKGSRNKYEYDPELGAIKLDRFLFSSMVYPTD
                     YGFIPDTLAEDGDPLDAMVTVSEPTFPGCVIPVKPIALFKMRDDKGIDDKVLCVPLQD
                     PAWNTFDVLDDIPDQLRDEIEHFFSVYKDLEQKLVTVDGWYPREDAIEVIRESRERHA
                     ENSRGS"
     gene            13452..13808
                     /locus_tag="VF752_05630"
     CDS             13452..13808
                     /locus_tag="VF752_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855063.1"
                     /translation="MRTAINFGILALVALLIVLIPGGGPAVGLVLTLLSIGFFVAMAF
                     FGVRLWRENRFALNTLSDFERGVLYGSIGLAFLTFTATPRLFDAGAAGALAWIALLAL
                     ASYGVFWVWTRYRAYG"
     gene            13888..14232
                     /locus_tag="VF752_05635"
     CDS             13888..14232
                     /locus_tag="VF752_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8855064.1"
                     /translation="MHTRALCGNGGQASVETVALLPVVVAVGLAGWQLAVAGHAAWLC
                     ANAARVAARAEAVGEDGRAAARSALPDSLERGLRVDRDGELLRVRVRVPWVIGHGQTP
                     LSLAASAGEVSR"
BASE COUNT         2015 a         4931 c         5258 g         2117 t
ORIGIN      
        1 acgaggctgc ggccaaggtg cgcctcttcc gagagttcga cccggcgtcg aacggctcct
       61 ccgacctcga cgtgcccgat ccctactacg gcggcgcgca gggcttcgac gacgtgctcg
      121 acctggtgga cgccgctgcg cgcggcctcc tcgagcgcat tcgctgagtg ggcctgctcg
      181 aggcgatcgc gagcgagacg ggtcgcgcgg tggcgggcgg ttgtcccgtg ggcggcggct
      241 gcatcaacga gggctggcgc gtcgacttcg aggacggcgg gcgcgcgttc gtgaagacgc
      301 ggcaggacgt cgcgccaggc gagtacgcga ccgaggccga gggcctgcgc tggctcgccg
      361 agccgcgcgc cgtccgcctg ccggaggtgc tcgccgtgcg cgatcagcag gagccgcgtt
      421 tcctcgcgct cgagtggatc gacgagggcc ggctcgacgc cgccggggcc gaggagctcg
      481 ggcgcgggct cgcgcgcatg cacgccgcgc acgcggacgc gttcggcggc ccgcggccgc
      541 tgcgcatcgg cccgatcgcg ctgagcaacg agcccctcgc cgactggccc tcgttctacg
      601 ccgagcgccg cctccggccg ctgatcggcc ccgcccgcga caacggctcg ctttcacgca
      661 ctggcgccga ggcggtcgag cgcgcttgcg agcggatgga ggagctgagc ggaccgccgg
      721 agccgcccgc gcgcctgcac ggcgaccttt ggggcggcaa cgtgctcgga ggcgccgacg
      781 gcggcgcctg gttgatcgac ccggcggcct acgccgggca tcgcgaggtg gacctggcga
      841 tgctgcgact gttcggcacg cccagcgcgc ggatcttctc cgcgtacgag gaggaggcgc
      901 cgctctacga cggtcacgag gagcgggtcg gcctctggca gctcttcccg ctgctcgtgc
      961 acgcggtgct gttcggcggc cactacggcg cgtcggtgga gcagacggcc cgccggtacg
     1021 cttgacgccg tggacctggg gctcgcgggc agggcgtgca tcgtgaccgg ggcgagtcgc
     1081 ggcatcgggc tcgcaacggc gcgcatgctc ggcgccgaag gcgcgaacgt actgctcacg
     1141 gcccgcggcg aagacgccct gcgcgaagcc gtcgatgaga tcggcagcgg cgcgcagggc
     1201 cttgttctcg acgtgcgcga tccggccgcg ggggaccggg cggtggagga gtgcgagcgc
     1261 cacttcggct ccgtcgacgt gctcgtgaac aacgcgggca cgagccgcaa ccggccgctc
     1321 gaccagctca ccgacgagga ctggcaggag cagtgggagc tgaacgtgat ggggccgatg
     1381 cggctcacgc gcgccgccgc gccgaagatg gccgcggcgg gctggggccg cgtggtgaac
     1441 gtgacgtcgt ccgcgggcaa gcgaccgtcc agcacgaaca tggcctacgc ggtgaccaag
     1501 gcggcccagc tctcgctctc ccgctcgttc gccgacctct acgcggcgcg cggggtgctc
     1561 gtgaacgcgg tcgcgcccgg tcccgtcggc acgccgctgt ggatggggga gggcggcctc
     1621 ggcgagcagg cggcgcaggc gcggggcgtc tcgctcgaac aggcactgga ggcgcagcgc
     1681 gcgaaggtgc cgctcggccg cttcgggact gaggacgaga tcgcggccgt gatcgtcttc
     1741 ctttgctcgg agcgcgcgag ctatgtgacg ggtgcggcct ggtcggtgga cggcggtacc
     1801 gtcgccacga tcctctagat cttcagcgtg atccggcgcg tgaccgtcgt cgtgcgcccg
     1861 gatgcgtcgg tcgcggtgat cgtcgccgtc gcgacgggct tgttgccgcg cttgagcgcc
     1921 ttccggagtg ccacgcgcgc tgaccccggg accgcgagcg tgatcgacgc cttgccgccc
     1981 gcggtgagct tcttcgtgac aggcgtgagc ttcactcttc cgcgcatgcc cttcaccttc
     2041 acgctgccgg cccccttcac agtgcaagcg ctcgagcacg acgccgagaa cttcacggcg
     2101 ttccgcgaga gctgcgtgat gaccggcgct ggcgtggcgg acgtcgcgac gggcgccggg
     2161 gcgggctcag gggcgggagc cggctgaggc tcggttgtga tgggagcggt ccgcgcctcg
     2221 gcgtcccacc gaaagctcac gctcgcctct gcgccttgaa gcgcattcgc cgcgcccgcg
     2281 ccgagcgcca ccgtgaagcg gtacgcgtgc gcctcaccgg gggcgaagtt cccgagcgcg
     2341 agttgcgaga gccccgcgaa cgagccgtca tacacgcgcg cggcggagct caggtcatcg
     2401 acgcgcagct gcagcgcact tatgagtgtc ccgacggtgt cgaccgcgga gaggaagaaa
     2461 gcgccgtccg cggagcctgc gttcgagatc gtcacggtgc cggacgcggt cccgccgggc
     2521 gccagccggt cggccgtcac gatcgcctgg cctgcacgcg agttgccgat ctgcacgtcc
     2581 cccgtcccgg cgctagcgag cacggccggg tgagggtcgc cgagcgcgac gtgcgcggcc
     2641 cccacggcga ttgcggccgt cgcagccacg acggcgatcg agctagaact ccgcatcgga
     2701 cgtccccgtc tcgctcactg cggtcactgg gctgaggacg ttgccggcgc ggtcagtggt
     2761 agcggcattg ggcgtccact tcatcgtccc cgcgctgccg gtgccggtcg cgccgctcgg
     2821 agtgcccagc gtcaccacga cgctgttccc ggagacctgc atcgacgagc tgttgaaggt
     2881 cacgtccgct gtcacgtagc tcttgccgcc gaggtcgagc ccggtgttgg ccgcggtcga
     2941 ggtgagcggg agcacggtcg tcgtaccact ggccagcacc tgaagcgtgt cgttcgtgct
     3001 gccgctggtg ttgtttgcga tccggacgga cacggcggtc gcgctgccgc tccacccgga
     3061 gaggatcgag agcggctcga tcggctcgtt gaacgtgaac gtgacggtgt cacccgcctc
     3121 ggccttgccg actgtcgcgc cgccgttcgc ggtctgcacg ccgctcgcgc tggggcctgt
     3181 gctgtccatc acgaccgaga agccggactg agtggtcgag ttgcccgccg agtcggtgag
     3241 cgagggcgtg tagctcttgg tccccgcggg cagcgcgctc gccgtgagct gtgcagagcg
     3301 gtaattgaac gtctggccgc cggcggcgta ggagccctgg ctgagagcca cggcggtctg
     3361 gccggtggtg acggagctca catcggcctt cacgctcgac acaccgctgg ccggattccc
     3421 gctgtcggct acgttcgcgt agacgtagta ggtcccgctc ggcctcacgt atccggtcac
     3481 gccgccgctg ctcttgccga tcacagacgc gctcgcgctg ggcgcggtcc agtcggcggc
     3541 ggccgagaac gacgagccgc tgctcgcggt ggtagcgctg aagctcgaga acgtcggcgc
     3601 accgggggcg cccaagccga tcccggcaag ggctgcgagc gccagtccgg cgaccgcacg
     3661 cctagtgcgc atgctcctcg cgcggccgcg ccggccgcca gatcgagacg agctcgagga
     3721 tgcagagcag gactgcgggg acggccacga gcgcgagcat tcctgtgcgg ctatggacga
     3781 gcgcgagtgc gtatccgagc ttccacacgc ggtactcgac ccggccgacc tcgccgccgg
     3841 tcggaacggt ccagcgctcc accgtgttgt tcgcatcgcc cttggtgacc acgtggacgc
     3901 ggccggcctg cgtgttcagg ctgcgtacac ggtgggtgat gaggcgcttc gaaccctccg
     3961 ggtcgcggaa ggtgacaacg tcgccgacgc gcatggcgct gggcggaatg cgactgtcca
     4021 cgacgacgtc gcccgtgtgg atcgcgggct ccatcgaacc ggacctgacg gtgaacggcc
     4081 gatgcccggc aagaacgggc agcgagactg cgagcaccat gccgattccg agtccgcacg
     4141 cggtccccgc gaggaacctg agcgcgatga gcgctgagcg cctcactcgt tcctcgcctc
     4201 ccaggtgaac gtggcgctgc cggtgaggcc ctgagccgcg gcgttgttgg caagggtcac
     4261 ggtgaagcgg tacgagtgcg actcgccctg cgtccagctc tcgggcgagg aggcgagggg
     4321 ctcgtcggat gccgcgccgt agctcgatgg gaacgctgcg agcgtgcccg agtaaacgac
     4381 gcctgcgccc tgcccaatgt agttcgtcgt gtcggcggtg aagccgccac acgacggaaa
     4441 cgacgcggcg gggtcggtgc cgcgtgtgac gaccaggttg aggtacggcg cgagtcctcc
     4501 ggtggccgag gcgtagaggc gcacgctcgc cggcagcgtt ccggcgtagg ttgccttcag
     4561 gcagctcgct tgagagtcgc cgggttttgc gccgctgagg gacagcagcg cctggccggc
     4621 gtcgttgtcc gagagcgaca cggtgccggc cgcgaagctg ttgccgccgt tagtggcggt
     4681 gctcgaaaac gccgaccagc tggccccgcc ggcgagcgcc gccgtgaggc cgagcgcgag
     4741 cccggtccgc agcatcttgc gtgtgcgctt caggttcatg ccggcttcac ctccggcaca
     4801 agcgagacat ctacgatgcg tcctcgccag ccagacgcag ctcctgcggg cagccacgcg
     4861 cgcacggtca gctcgcgccg ctcgccactt ctcagcctca gcccgccggt cgatccccgc
     4921 agccgcgaga gtgggtcgcg gacgacgact cggcggcccg ctcgaagctc gagccacagc
     4981 gcagcgtcgg tgtcgtggag cgaaggcgca gcgcggaagg cggcccggag cggcagaccc
     5041 gtctgattgc ggaccgacat cgtgccggtg agcggcggac cgccgggccg cagcgcgctc
     5101 acgtgagcga gcggtgctgc gggcagaacc tcgagctccc cgctcgacgc cgaccgcaga
     5161 gtcaggtcga gcccaagcgt gcccgcgccg gcctgcaccc gccagccaca cagcagggcg
     5221 gccgcaagtg caagccccgc gagcacgccc aggccaccct ggcgcaggag cctccgtacc
     5281 gacaacgcgc cgctactggt tgcgggcttc ccagacgaac gagtgcgcgc cggtgctcgc
     5341 gcccttcgcg ctgttcgtgt cctgcaggct gagcgaaacc cggtacacga ccgagtcgct
     5401 cgtagcccac ttcgtgctgc cgccggggtt gaaggacagc ccgttcgcgt acgagttctt
     5461 cgcggccgcg aactgtgcca gggtgccgga ccaagcggtc gcacccgagt cgggcgtgaa
     5521 gcccgtgcag cccgggaacg cggcgcccgg ctgcgtgccc ggcgttacgg tcatgtcgag
     5581 gtactgaccg agcgagccga ccgagtccgg cgtgtagagg tgcacatcgg agtcaagcga
     5641 cccgctgtag gtgaccttga tgcacttggt gaccgagtca ccgggcttct ggttcgagac
     5701 gttgtagagg aactggccgg catcgttcga gccgacgttg acggtgccgg cggcgaacga
     5761 gtttccggcg ttcgtggtgg tgctcgagaa cgccgagaag acgccgaacc cggctgcgct
     5821 gccggcgatc cccagcacga ggatcgacag caggaccttc ctgctcttgg acatgacgga
     5881 ggactcctgg agggttggtg ttggagtcag ggccgacgga gggacattcc gtgtcagagc
     5941 tctgacacag ctctattcgg ccccgcgccg gagaacttga gaggcttccg ccccttgcaa
     6001 gccggcccgc acacgcagaa cagacgagtc ctgatggctc taatgttctt tccccgcggc
     6061 ggatcggcgc acgtgaaccg ctacatggcg cgcttcctgc cgcggtcggg ctgggacgtc
     6121 acgctcgtga ccgggtcgct cggcgcgccg ggcgcgctgt cgaacgcgga gactttcttc
     6181 gaacggctcg acgtgcggcc ggtcgactac accgctgcga tgagcgcacc cgacccgctc
     6241 ctcgcggatc cgcccttgca cccgtcgtac gaggagcgcc cggacgcgcc cgacctcgtg
     6301 ttcgcgcggg tggacgacgc ggcctacgag cacctggtgg ccgcttgggc gggcgcgctg
     6361 catgacgcgg gcgcggctga agccgacgtg ctccacctga accacctgac gccgatcaac
     6421 gaggcggccg agcgcgagtt tcccggtgtg ccgcgggtcg gacatctgca cgggacagag
     6481 ctgctgatgc tgaatcgcat cgatgccggc aacccagacg gctgggagca cgccgaggag
     6541 tgggcggagc gcatgcgcgg atgggcgcgc cgctgcgagc ggttgctggt gctctcgcct
     6601 gatgccgtgg agcgggttcc ggcgctgctg ggcgtggagc ccaagcgcgt ggtgtgggcg
     6661 ccgaacggct tcgagcccga gggcttcgag cggcggccgg tgcgcgggga cgcgcggctg
     6721 gcgttctggc gccgctggct cgtcgaggag ccgcgcggct gggacccgga gtcgcaacgg
     6781 ccggggagcg tccgctactc ggaggtcgat ctggcggcgt tcgctggtga ctcgcccgtg
     6841 ttcctgtacg tgggccgcta tacggaagtg aagcggatcc cgctgatgat ccgcgcgcac
     6901 cagcgcgcgc tcgccgccac cggcgcccac gcgccgctcg tgatcctcgg cggctaccct
     6961 ggcgagtggg aggggcagca tccgctcgag gtcatccgcg agacgggcgc gcgcgacgtc
     7021 ttcctcgcgg gctggcgcgg gcacgacgag ctgcccgacg ggctgaacgc ctcagacgtt
     7081 gtggtgctgg cctcggttca cgagcagttc gggcaggtgc tcgtggaggg gatggcctgc
     7141 ggcctgcccg cgatcggcgt gaacgcacac ggtccggcga cgatcatcga cccgggcgag
     7201 accgggtggc tcgccgagcc ggacgacgag gagtcgctcg cgcaggcgat gaccgaagcc
     7261 atcgcgtcac ctgagcgtcg ccgcgagctg ggcgagcgcg cgtacgtggc tgctcgccag
     7321 cgctactcgt ggcctgcgct ggcggagcgg gtcgcgcgcg tgtacgacga ggtggccgac
     7381 ggccgctgac cgaccggcgc gcgtcctatc gtcgtcgccc catgagcgac gcgatcccgt
     7441 accgaatcag gcgctccgac cgggcccggc gggcccggat cgtggtcgac gccgagggcg
     7501 tggaggtggt cgtgccgcgc cggatggcgc tgcgtcaggt cgagccgttc gtcgccgagc
     7561 acagcgcgtg gatcgagaag acgcttcgtc gataccggcg cgcggaggaa gcgctgccgc
     7621 ggccacggct cgaggacggc ggctgggtgc cgtacatggg ccgggtgctc gagctgcgcg
     7681 tgcgcgtcga gcccggccgg gtccgcgact cggtggtgcg ccgcggcgag atggcgctcg
     7741 aggtgcgcgt ggcctccggc gacgaggact cggtgcgctc ggcgctcgag cgctggtacc
     7801 ggcggcgcgc gcgcgtggag gtcgccgctc ggctcgacgc cgcgacccgt cgagcgggca
     7861 cgagctaccg ctcgctcacg atccgcagcc agcggacgcg ctgggcaagc tgttcctcta
     7921 acggctcgat gagcttcaac tggcggctga tgctggcgcc cgaggcagtg ctcgactacg
     7981 tggtcgagca cgaggtggcg cacctggagg tgctcgacca ctcgccgcgc ttctggcgcc
     8041 tgctcgggtc gcgctgcccg gatgcgcggg tgcacgagcg ctggctgcgg aagcacgggt
     8101 cggcgctcag gctgtaggga ggctgaacag aagcacttac ggagatcctc ggggtgcggc
     8161 ccgcggccgc tacctccgat gtgtggaagg tttgtccggc tatagcgggg caaagtctcc
     8221 acagatcgac cgactcggcc ccgcaggagt gtgtgctcct agcccttaag tgctgctttt
     8281 ccggatcgct acccggcccg gagtcctact cccagcggaa cttcgcgatc gcaaagaaca
     8341 gccccaccgc agtccaagcc aggatcacgc ccagggccgt gagatggtgg ctcactccct
     8401 ggcccgtcac gagcccgccc gacagcccgt cgatcacgtg cttcagcggc agcgcctcgg
     8461 cgatcccgct cagcaccgcc ggcaggttgt tcgcgtcata gaacacgccg gagatgaaga
     8521 tcgccggcag gaagaccgcg ttggtgtagg ccggcgcggc gtcgaagttc gggatcgcgt
     8581 gcgagaaagc cacgccgagc gcgccgaagc acgtgacgcc gagcagcgtg aagaggatga
     8641 gcgcgcccca gtcgcgtggc cacgacacgt ggtaggcgac gtgcccgatc accaccacga
     8701 gcgcaacctg cacgaccgcg ttcgcaaccg cgttgccgat cacacccgtg aaataggacc
     8761 ccgtgggcat cggtgtgccg tagatgcgct tgaggatccc ctgctcgcgg aggaaggtca
     8821 ggttgaacgc aagcgcggtg aaagtcgtcg ccatcaccgc caagcctgcg agccccggca
     8881 cgagcacgtt cagctccttg cggtcggcgc cgaacgcgga cgcgatcaac acgaggaaga
     8941 gcaggggcag tgcgaagttg aagaaggcgg cgctcgggtt gcgccagaac atccgccgct
     9001 cgaagcggaa ctggcgccac gcgaggacgg cagagctcac tccgcgtcct ccgctgtcag
     9061 ctcgaagtag acgtcctcga gcgtcgggcg gcccacgtgc agtccctcga ggcgctcgcc
     9121 acgcgcgagc gcgtcgccgg tcagctcgtg gagcaacgtg gtcgggtcgt cggtcacgtg
     9181 ctcaacgcgg tcgccgttgc gctggtagga gacggtgtac cggaagcgtg acggcgctag
     9241 ctcgcccggg gagccctcgg ccacgaacac gccgtccttg atgatcgcca cgcggtcggc
     9301 gagcgactgg gcctcgtcga gatagtgggt ggtgagcacg atcgtcttgc cgagctcctt
     9361 gagcgccgta atcgcgcccc aggcggtgcg tcgagccgcc gggtcgaagc ccgtggttgg
     9421 ctcgtcgagg aagatgagct cgggatcgcc gacgagggcg agcgccaggt cgagccggcg
     9481 cagctgcccg ccggagagat cgcgcgcgta cgcgtcgcgc ttctcttcga ggcccacgac
     9541 ggcgatcgtc tcggccgggt cgcgcgggtg cgggtacatc gcggagaagt gctcgaccgc
     9601 ctcgcgcacg gtcgcgcgcg ggtagatgcc cgagctctgc agcacgatcc ccacgcgctc
     9661 gcgcagcgtg cggtcgcgcc gctcaggatc aagtccgagg acagagaccc ggccgcctga
     9721 gcgcttgcga tagccctcga ggatttcgac ggtcgtcgtc tttccggctc cgttcggccc
     9781 gagtaggccg aacacctcgc cgcgctcgac ggcgaagctg acgccgcgca cggcctcgtg
     9841 gtcgccgtag ctcttgcgca ggtcctcgac ctcgatggcg cttccgttca cggcgtggac
     9901 tatggcaagc tgagcgccct tggcattcgc ctgcatcgac gtcgggagca acacgactcg
     9961 gctgctggtg gccgatgtcc gggacgggcg cctgcgggag ctctacgcgg aacgcgtgtt
    10021 cacgcgtatc ggcaagaacc tgctcgacca cggcgaggtg gagcccgcga aggtcgccga
    10081 gcatgcgctg gtggtcgcct cgcaagctga caccgcgcgc gagctgggcg ccatcggcat
    10141 cgacgtggtg ggcacggcag tcgttcgcca ggcgcccaac gccgccgacc tcgtcgcggc
    10201 cgtggacgag acttgcgggc tcccgatgcg catcctcgag gcgcgcgagg aggcgcgcct
    10261 gtcgttcgcc ggcgcggttg ccacgcacga cgccccggtc gacggcacgc tcgcggtgct
    10321 cgacgtgggc ggctggtcga cggaggtggc gctcggcacc gcggccggcg gcatcacctg
    10381 gtgggagtcc ttcccgctcg gctcgggcct gctcgccgac ctgcacctgc gctccgaccc
    10441 gcccgaccgc gaggacgtcg agaacatccg cgcgcacgtc gaggaggtgc tggacggcct
    10501 cgacctgtcg cacgccgagg gcgcgatcgc ggtcggcggg gccgcaggat cgctgcggcg
    10561 gctcgtgggt cgtgacctcg tgcccgagcg gctcgcgcgc tgcctcgaca tcctgctcgc
    10621 caaccgcgcc gccgatgtcg cggcacacgt ggacctgcac caggagcgcg tgcgcctcat
    10681 gccggcgggc gtgatcgtgg tgagcgagct ggtacggcgg ctcgcggtcc cgctcgagct
    10741 cgggcggggc ggcctgggcg agggcgtcgt catggcgctc gcgcgggctg cgcagtagct
    10801 tcgcgctcag cgatacggcg cggtagcctg cgtgcgacgg gccgagggag gcgcgggtct
    10861 caaggtctgg ctccctcgga acgatgaggt cgttggatgg gtactgctga gcacatcgag
    10921 ccgacccggc accggcgcgc ccggctgccc cgctgggcct tcgcggcgct tggcgccgcc
    10981 gtcgctctcg ccctagtcgc ggtagccgtg ctcgctctcg gcggcggcgg ctcatccgcg
    11041 agccgctcga acgccaagtc atcgaacgct gccaccgcga aggcgctcac cccggagcag
    11101 aaggcggcgc tcgccgacca cagcgctgac aaatcgctca aggcgcaccc gcaggtcgtg
    11161 caggtgggcg gcgccgggag cgacaccggc atcaccgctg aggaccaggt ggcgggcatc
    11221 tctacgggtg ccaacgcccc gcatgccccc accgacgcgg agatcaagaa cgagctcgcc
    11281 gccttcaagc gccacctcgc caccggcgtc ggcctgcgcc acggcccggt cgcccaggtg
    11341 ctctccaccg gcgaggcggt ggcgccgatc gacgcgccgc cgatcgtcga cacgatcatc
    11401 cgcgcaggca acgccatcgc caagacgccg tacaagtggg gcgggggcca cggcgcctgg
    11461 tccgacaccg gctacgactg ctcgggctcg gtctccttcg cgctcgccgc ggcgggcctg
    11521 ctcaacagcc cgctcgactc gaccggcctc tcgcagtggg gcgcggcggg cgagggcagg
    11581 tgggtgacga tctacgcgaa cggcggccac gccttcatgg tggtggcagg cctgcgcttc
    11641 gacacgagcg gccgcagcgg cccgaccggc acccgctggc agcccgcgat gcgctccacg
    11701 agcggcttcg tggcgcgcca cccgccgggt ctctagacgc cgacgcctcc gcgaccttcg
    11761 ccgcccgcga aacgcgccgc gccctgctgc gcgatgcgca gcagctcggg gccagcgttc
    11821 gcctcgagcg cgagcccttc gtcgagtcgc aagccgagcc cggcgattgc ggcgtggcgg
    11881 tcggcgagca tcgtctcctg ggggaagccg gcgagcgtct cggccagctc gagcgagcgc
    11941 tcgagtgcct tgccgtggtc aacgatctcg ttcacgagcc cgatctcgcg cgcctcctcg
    12001 acgcccacca tcctgcccgt gaggatcatc tcgagcgctc ggccaaggcc gaccacgcgc
    12061 gggagacgct gcgttccgcc gtcgatcagc ggcacccccc agcggcgctc cggaaggccg
    12121 aacgtcgagg tcgtcgtggc gatgcgaagg tcgcaccaga gcgccagctc gagccccccg
    12181 gcgaggcacc agccgtcgat cgccgcgatc gtcggcttcg agggcgtgcg ccgggtgaag
    12241 cccatcgggc cgtcggcgct cgagagccgc ggcccgaggc tctcgatgtc cttcaggtca
    12301 gcgcccgcgc agaaggcgac accgcccgcg ccggtgagca cgagcgcgcg cgcctcgtca
    12361 tcagcctcga agcggtggaa gccctctccg agcaggtcgg cagtgggacc gttgaccgcg
    12421 ttgcggcggt ccggccggtc gatcgtgagg accgcggccg cgccgattcg ctcgtatgtg
    12481 acctcgggca cgcgggaagc ctctcagaaa agcgaagcgc ggcccgtggg gcgggggacc
    12541 ccgggccgcg cgctgaacga ggagggatga ggaactcgtt cgcggccgat actgcccgcc
    12601 gcgctgccgc ggaatgtgaa aagtccgata aagcgggcta ccagcccgac gtgcggtagc
    12661 gcgaggcggc ggcccgcttc tcgtacttcc gcctctcgag cgctccctgc agccagctcg
    12721 cgaggatgac gaaggcgatg aagaagatca cgagcccgag gccgaacgcg gtcacgatcc
    12781 ggtcgtcggt ctcgccgagc aggccctcgc cgtcgtccga cgcgaacgcg gtggcctgga
    12841 gggccagcac gaacgccgcc gagccgagaa gtgtgatgag ggcgcgcctg cgcatcgcgg
    12901 gaaaagataa cgattcggct cgccgggtaa tctcgccacc gtgccggacg attcgctcct
    12961 gatggtggtt gagatcccca aggggagtcg caacaagtac gagtacgacc ccgagcttgg
    13021 cgcgataaag ctcgaccgct tcctgttctc gtcgatggtc tatccgaccg actacggctt
    13081 catccccgac acgctggccg aggacggcga cccgctcgac gcgatggtca cggtgtccga
    13141 gccgacgttc cccggctgcg tgatcccggt gaagccgatc gcgctcttca agatgcgcga
    13201 cgacaagggg atcgacgaca aggtgctgtg cgtcccgcta caggacccgg cctggaacac
    13261 gttcgacgtg ctggacgaca tccccgacca gctgcgcgac gagatcgagc acttcttctc
    13321 ggtctacaag gacctcgagc agaagctcgt cacggtcgac ggctggtacc cgcgcgagga
    13381 tgcgatcgag gtgatccgcg aatcccgcga gcggcacgcc gagaacagcc ggggctccta
    13441 agcttcggcg tatgcgtacc gcgatcaact tcgggatcct ggcgctcgtc gcgctgctga
    13501 tcgtgctgat cccgggtggc ggtcccgcgg tcggccttgt gctgacgctg ctctccatcg
    13561 gcttcttcgt cgcgatggcg ttcttcggcg tgcgcctgtg gcgcgagaac cgcttcgcgc
    13621 tgaacacgct gtcggacttc gagcgcggcg tgctgtacgg ctcgatcggg ctggcgttcc
    13681 tcacgttcac cgccacgccg cggctgttcg acgccggcgc ggcaggggcg ctcgcgtgga
    13741 tcgcgctgct cgcgctcgcc tcgtacggcg tgttctgggt ctggacgcgc taccgcgcct
    13801 acggctgagc ccacttttca cgcccttgta gcggagctgt aacgctcgtc acccacgtgc
    13861 ctgccgcggc cggcgaggct gccgcgcgtg catacacgag ctctgtgcgg aaacggcggg
    13921 caggcgagcg tcgagaccgt cgcgctgctg ccggtcgtcg tcgcggtcgg gctggcgggc
    13981 tggcagctcg ccgtggccgg tcacgcggcg tggctgtgtg cgaacgccgc gcgcgtggcg
    14041 gcgcgagcgg aggcggtcgg cgaggatggc cgcgctgcgg ctcgatccgc gctgcctgac
    14101 tcgctcgagc gggggctgcg ggtcgatcgc gacggtgagc tgctgcgcgt tcgcgtccgc
    14161 gtgccgtggg tgattggcca cggtcagacg ccactctcgc tagcggcgag cgccggcgag
    14221 gtgtcgcgat gatgcgctgc gagcgcggcc agggctcggt ggaattggtc gcggctgtgc
    14281 ctgcgctgct gctggtggcg ttcgtgtgct tccagctcct c
//
LOCUS       DASVXS010000100        10995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_946483, whole genome shotgun sequence.
ACCESSION   DASVXS010000100 DASVXS010000000
VERSION     DASVXS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946483"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VF752_05640"
     CDS             <1..189
                     /locus_tag="VF752_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855065.1"
                     /translation="AALRLMRFMSDLREAMWGVVQSAVSDLDFDFTGYAAQHFERLHR
                     TAADPRFAESLEEARAAA"
     gene            176..2635
                     /locus_tag="VF752_05645"
     CDS             176..2635
                     /locus_tag="VF752_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855066.1"
                     /translation="MPPPELPGAARCVIVGGGVGGTSIAYHLAELGFEDVVLLERSQL
                     TSGSTFHSAGLVGQLRGSVSLTKIMMHSVELYRRLGAETEFDPGWVECGSLRLASSEE
                     RMEELRRQAGWAKTFGLPLDLITAEEARERFPLMSTDGVLGAAWLPTDGYLDPSQLTY
                     ALADGGRGGGCRIFTGTRVVGIDVDRGAVRRVRTNRGDIECELVVNAGGMFAAEIGRM
                     AGVRVPVIPMSHAYLVTQPFRERDGTHLPTLRDPDLLIYFREEGGGLVMGGYERNSAP
                     AFLGGDGLDHIPGDFNGRLLEDDWDRFEEIVENSKRRVPVMDEIKVTRMINGPEAFTP
                     DNEFCLGESEVRGFFVAAGFCAHGLAGAGGIGKVMAEWIADGEPSLDLWEMDIRRFGS
                     QYRSPSYTLARVRETYETYYDIRYPNHEREAGRPLRMSPANAWHRDHGAVFGEKSGWE
                     RVNWYASNDAAGDESLRPRGWAGQHWAPAIGAEHVATRERAGIFDESSFAKMEIAGPG
                     AAAFLESLCDNRVAREVGRITYTQMLNTRGGIECDFTVARLAEERFSIVTGTAFGNHD
                     MTWIRRHLPSDGSVQVHDVTSAWACFGIWGPLAREILQPLTPSDLGNGSFPYMSVREI
                     TVGHVPVRALRVTYVGELGWELYCPAEYGAGLWRLLWDAGEPHGMAAGGYRAIDSMRL
                     EKGYRVWGADITPDETPYEGGLGFAVKLDKDGGFIGRDALVEARENGPRLKLCCLVLE
                     DPRSVALGNEPVRVDGAVVGRVTSGGYGYTVERSIAYAYLPPEHAEPGTNAAVEIFGR
                     WVEGAVAAEPLFDLDGARIRA"
     gene            2639..3076
                     /locus_tag="VF752_05650"
     CDS             2639..3076
                     /locus_tag="VF752_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855067.1"
                     /translation="MDSDSLRSNRSIPSSPVIPVLVYPDVREAVEWLADAFGFVERVR
                     IGENHRSQLKVGEGAVIVADVRNDRRPPRPGEETHSVVVRIEDANAHCERARTHGARI
                     LMEPTDFEYGERQYLAEDHAGHRWTFSETLRDVAPEEWGGTTT"
     gene            3055..3921
                     /locus_tag="VF752_05655"
     CDS             3055..3921
                     /locus_tag="VF752_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855068.1"
                     /translation="MGRDDHVTAVHSILPERLTQVVDAELQAAGVRHVRHGEADPVTA
                     AEAAAADTDALALIGPFRSAAVAEAVEATAPAGLPLLAPVATWAGVTRDDEPGCDDPA
                     RHRGTVLRLVARDTEVAHRIAEHVRANGWHALVVAGEHDYGRQLDGQLRIAGLPRAED
                     AGDADLVVLGGLVGEAEIKQAAATVPLPLIAFDGVQGAELGDREVHLALPFAPVDGVP
                     PRDLFEGLDQARRASRLAIRALDDGASDRPAMLDALRRLGGFDQHGDPLDPPVWLWRA
                     GPGWNVEPDRPL"
     gene            complement(3926..4639)
                     /locus_tag="VF752_05660"
     CDS             complement(3926..4639)
                     /locus_tag="VF752_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX8855069.1"
                     /translation="MERSTALEAVFEPVAPPTTFEETVERLGTAIRLGLLPASSQLPP
                     ERDLALELGISRSTLRQAITALVQSGHLVSVRGRNGGTFVADSPPLAVPGTAKPDDEY
                     VQSVLDHRVAIEVGCVLLAAERAHHEDLDRMLELVDTMTDAIEFEDYRRADMRFHVAL
                     AEAARSPRLVAEMTDVQGRMGELIALIAHPEEVLTRSNEQHRHLVALLSRGDSGAAVK
                     LIRKHIEGTEHILAGLFPG"
     gene            complement(4694..6034)
                     /locus_tag="VF752_05665"
     CDS             complement(4694..6034)
                     /locus_tag="VF752_05665"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX8855070.1"
                     /translation="MATAQEQTTVEQVLARWREQGIRNVRFELPDIHGTSRSKLIPID
                     HAASYARSGLNMYGGTVVLDTRSDVVPGTLYNEEVAYADQRLKPDPSTAKVVPWADAT
                     GRMICDTFWDDGRPLGAAPRQVFKRVLDRCHELGYEALIGVEPEFYLLDGETKQPLFQ
                     GYHIFNTVRNTYVPAIQRIVDDSRAFGLDIITANCEYAGSQWEINFGPGIGMNGPDDA
                     FSFKNGVKEIAHLEGYLATFMSKPFADGAGCGCHNHICLLELEGGANAFSDESDEYRL
                     SPVGRAFIAGQLRHARSIYTLLAPTINCLKRRRTHTFSPTNVSWGPEDRSAFVRVKGG
                     SPESRHIENRAPTGMSNPYLACAGLLAAGLLGVTDELALEPPAAPPAEEDESKPKLPT
                     TVEEGLEALESDERIVELLGDEFVKAYGVMRRYELQRFYDHVTDWEREEYLELY"
     gene            complement(6040..6816)
                     /locus_tag="VF752_05670"
     CDS             complement(6040..6816)
                     /locus_tag="VF752_05670"
                     /inference="COORDINATES: protein motif:HMM:NF044879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855071.1"
                     /translation="MNPHAATGLRALAGIGEVRAALQQKDNLCGPFHAARVLRDAGFD
                     VDQDLIALTAGTVLPDEDPQASVPTGAVSRRDYRVELPLAAEPAVSGTAAADLAQAIE
                     QVSRGELRCVPLRGEWSAETVERLVDGAGDARLIANVRTGHFWGARPPLETLLRHLAG
                     EEVDPPSHEWDVGHFVELVSLVRGAGGSLVVVCDSYPTFGWQAHHLQPPAAVAAALLR
                     GDGREGGVLAVTDAADAARVEQLAAELGLDVETWDNGTRR"
     gene            complement(6824..8389)
                     /locus_tag="VF752_05675"
     CDS             complement(6824..8389)
                     /locus_tag="VF752_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8855072.1"
                     /translation="MTIESQPPGGGASAAEPPRGVEYRHEGGLVIESVDVNQRDDVHR
                     LHAGAVGLVGVLFLAVTGAAPLTAMLGNVPYAVGFGNGIGAPSGFWIATIVLTVFSVG
                     YVAMARKLTASGGFYSFISHGLGRPVGLASGWTAMACYMIFEASLMGIFAFFAKDTFN
                     RFLHIDIGWGWYAFAGLIIISLLTYFDVRISARVLGVALITEVLILLIMDFGILFSGG
                     GPDGLSAKPLSPSEGFSSVGGGAAAVGIFFAFWSWVGFEATVNYGEESRNPKKIVPRA
                     TYIAVIALGIFYTFTSWMVISGWGHDEAIKQAQNDATNFFFTPTQDFVGTLAKDIMQW
                     VIVTGSFACGMAFHNAASRYFYNLGRERVFPAALGRTHPKWHSPYAGSLFQSAMAAIL
                     VAIFVILDKDPYVALYGWLAVLGTFGILLVQSLTAISSFSYFERYHKEDVHWWRTRLA
                     PILGAAGMIAVLVLLITKLTEIGGDVGFIKAVPFIVAGWFVLGLLLAYYLKLRDPRRY
                     QVVGRMIDQGVDT"
     gene            complement(8475..9209)
                     /locus_tag="VF752_05680"
     CDS             complement(8475..9209)
                     /locus_tag="VF752_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX8855073.1"
                     /translation="MTAHWEPVIGICAVRETARWSFWEQPAHLVADSYVSAVQRTGAI
                     ALILPIDPRAPEALLDRIDGLLLIGGADIDPESYDEDRDPRTEATYPERDSFELRLLR
                     GALEREMPVFGICRGMQLMNVAFGGTLLQDLTAPDGTNPHRKALGSFEGTEHEVRLDR
                     GSLVARASGEEVHVARCHHHQGLDKVGEGLSVSGRAVIDGLPEAVEASDGRWVLGVQW
                     HPEADERSRLFNDFANAARSRARSLA"
     gene            complement(9213..10550)
                     /locus_tag="VF752_05685"
     CDS             complement(9213..10550)
                     /locus_tag="VF752_05685"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX8855074.1"
                     /translation="MTEADTSTFAQPAAPARVPDDAEFVLLGIPDMHGQIRGKALRPS
                     AFESALRSGTVMTDLLLALDPTDEPITDYESFGIRSGAGDMIVQPDIETLRTMAWRPG
                     WSVCLARPSWPDGSACMLDSREVLRHVLADMSELGYDVTAAFEYEIRLRDENGEFLSS
                     GISYSVGEIARFDDFIRQLGPALDGLGVDLTAVHTEAGPGLLELNIAARPGLRAADDA
                     AFVKLAAKEVAAAMGLTATFLAKTVPGQEGSSGHLHLSCWADGENAFASSGDGELGGV
                     FASAVAGVLDHLPGASLLMNPTVNSYKRLVPGWFAPVNVSWGLENRSAAVRAIRSADH
                     PERCRIECRRPGADANPYLALAALAAAAADGIRRGASPPEPVVGDASEREDLQPLPGS
                     LESAIAAFRADAVLREALGEEFSAYFEVSREWELKKWRESVSDWERERYERAV"
     gene            complement(10547..>10995)
                     /locus_tag="VF752_05690"
     CDS             complement(10547..>10995)
                     /locus_tag="VF752_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8855075.1"
                     /translation="LGHTWDGAPLPCGVGLAVLEYLEDNALLERVRERGPSLRDELEQ
                     ALDGSEIVGEVRGRGYLLGVELVDPRDGESPLPVEIDAAEMVDQTAFEHGVLVTSSHS
                     TPDGFTGEQTLLAPAYNSTDDDLAAMLDRFAAAMADVEDQVKERLA"
BASE COUNT         1712 a         3836 c         3805 g         1642 t
ORIGIN      
        1 gccgccctgc ggctgatgcg cttcatgtcc gatctgcgtg aggcaatgtg gggcgtggtg
       61 cagagcgcgg tgtccgacct cgacttcgac ttcaccggct acgcggcgca gcacttcgag
      121 cgactgcacc ggacggcagc cgatccgcgc ttcgcggagt ccctcgagga ggcgcgtgcc
      181 gccgcctgag ctacccggcg ccgcacgctg cgtgatcgtg ggcggcggcg tcggaggaac
      241 atcgatcgcc taccacctgg cagagctcgg cttcgaggac gtggtgctgc tcgagcgaag
      301 tcagctcacg tcaggttcca ccttccactc ggctggcctc gtcgggcagt tgcgcggctc
      361 cgtctcgctc accaagatca tgatgcactc ggtggagctc taccggcgcc tcggcgccga
      421 gaccgagttc gaccccggct gggtggagtg cggcagcctg cgcctcgcgt cgagcgagga
      481 gcggatggag gagctgcggc gccaagcggg ctgggcgaag acgttcggcc tgcctttgga
      541 tctgatcacc gcggaggagg cgcgggagag gttcccgctg atgagcaccg acggagtgct
      601 gggcgccgcg tggctgccca cggacggcta cctcgatccc tcgcagctga cgtacgcgct
      661 ggccgacggg ggtcgcgggg gtggctgccg catcttcacc gggacgcggg tcgtgggcat
      721 cgacgtggac cgcggcgccg tgcgccgcgt gcgcaccaac cgcggcgaca tcgagtgcga
      781 gcttgtggtg aacgcgggcg gcatgttcgc ggcggagatc gggcggatgg ccggcgtgcg
      841 cgtgccggtg attccgatgt cgcacgcgta cctcgtgacc cagccgttca gggagcgcga
      901 cggcacgcac ctcccgacgc tgcgcgaccc cgacctgctc atctacttcc gcgaggaggg
      961 cggcgggctc gtgatgggcg gctatgagcg caacagcgcg cccgcgttcc tcggcggcga
     1021 cggactcgac cacatccccg gcgacttcaa cggccgcctg ctcgaggacg actgggaccg
     1081 cttcgaggag atcgtcgaga actcgaagcg gcgcgtgccg gtgatggacg agatcaaggt
     1141 cacgcgcatg atcaacgggc cggaggcgtt cactcccgac aacgagttct gcctcggcga
     1201 gtcggaggtg cgcggcttct tcgtggcagc cggcttctgc gcgcacggcc tcgccggggc
     1261 gggcggcatc ggcaaggtga tggccgagtg gatcgccgac ggtgagccgt cgctcgatct
     1321 gtgggagatg gacatccgcc gcttcggctc acagtaccgc tcgccctcgt acacgctcgc
     1381 gcgcgtgcgt gagacgtacg agacctacta cgacatccgc tatccgaacc acgagcgcga
     1441 ggccgggcgg ccgctgcgca tgtcgccggc gaacgcctgg caccgcgacc acggggcggt
     1501 gttcggcgag aagtcgggct gggagcgcgt gaactggtac gcctcgaacg acgcggctgg
     1561 tgacgagtca ttgcggccgc gcggctgggc cgggcagcac tgggcgccgg cgatcggcgc
     1621 cgaacacgtc gcgacccggg agcgtgctgg gatcttcgac gagtcgtcgt tcgcgaagat
     1681 ggagatcgcc gggccgggtg ccgcggcgtt cctcgagtcg ctgtgcgaca accgcgtggc
     1741 ccgcgaagtc gggcgcatca cctacacaca gatgctcaac acgcgcggcg ggatcgaatg
     1801 cgacttcacg gtcgcgcgcc tggcagagga acgcttctcg atcgtgaccg gcaccgcgtt
     1861 cggcaaccat gacatgacgt ggattcgccg ccatctgccg tcggacggca gcgtgcaggt
     1921 gcacgacgtg acgtcggcct gggcgtgctt cggcatctgg ggaccgcttg cgcgcgagat
     1981 cctccagccg ctcacgccct ccgacctcgg caacgggtcg ttcccgtaca tgagcgtgcg
     2041 cgagatcacc gtgggccacg tgccggtgcg ggcgctgcgc gtgacctacg tgggcgagct
     2101 cggctgggag ctgtactgcc cggcggaata cggcgccggg ctatggcgcc tgttgtggga
     2161 cgcgggcgag ccgcacggga tggcggcggg cggctaccgc gcgatcgact cgatgcggct
     2221 cgagaagggc taccgcgtgt ggggagcgga catcacgccg gacgagacgc cttacgaggg
     2281 cgggctcgga ttcgcggtga agctcgacaa ggacggagga ttcataggtc gtgacgcact
     2341 ggttgaggcg cgggagaacg ggccgcgcct gaagctgtgc tgcctcgtac tcgaggatcc
     2401 gcgctcggtg gcgctcggca acgagcccgt gcgcgtggac ggcgcagtcg tcggccgggt
     2461 gacaagcggc ggatacggct acacggtcga gcgctcgatc gcgtacgcgt acctgccgcc
     2521 cgagcatgcc gagccgggta caaatgccgc cgtggagatc ttcggacgct gggtggaggg
     2581 cgcggtcgcg gcggagcccc tgttcgacct cgatggcgcg cgcatccgtg cgtaacgcat
     2641 ggactcagac tcgctgagga gcaatcggtc gatcccctcc tcaccggtca taccggtgct
     2701 cgtctacccg gacgtgcgtg aggcggtcga gtggctggca gatgcattcg ggttcgtcga
     2761 gcgggtgcgg ataggcgaaa accatcgctc gcagctcaag gtcggcgagg gagccgtgat
     2821 tgtggccgac gtccgcaacg accgccgccc gccgcgcccg ggcgaggaaa cccactcggt
     2881 cgtggttcgc attgaggacg cgaacgccca ctgcgagcgc gcacgcactc acggtgcgcg
     2941 gatcctcatg gaaccgaccg atttcgagta cggcgagcgg cagtacctgg ccgaagacca
     3001 cgccggacac cgttggacgt tctcggagac gctgcgggac gtcgctcccg aagagtgggg
     3061 cgggacgacc acgtgacggc ggtccactcg atcctcccgg aacggctcac gcaagtagtg
     3121 gacgccgagc tccaagctgc gggagtacgc cacgttcgcc acggagaggc ggacccggtg
     3181 accgcagccg aggccgccgc cgcggacacg gatgcgctgg cgctgatcgg ccccttccga
     3241 tcagccgccg ttgccgaggc ggtcgaggcc acggcgccgg ccggcctgcc gctgctcgcg
     3301 ccggtcgcca cttgggccgg cgtgacgcgc gacgacgagc cgggctgcga cgacccggcg
     3361 cgccatcgcg gcaccgtcct acggctcgtg gcgcgcgaca ccgaggtggc ccacaggatc
     3421 gccgagcacg ttcgagccaa cggatggcac gcactggttg tcgcaggcga gcatgactac
     3481 gggcgccagc tcgacggtca gctgcggatc gcaggcctgc cgcgcgccga agacgccggc
     3541 gatgccgacc tcgttgtgct gggcggactc gtgggcgagg cggagatcaa gcaagcggcc
     3601 gccaccgtgc ccctgccgct tatcgccttc gacggggtgc agggcgcgga actgggtgac
     3661 cgcgaagtac acctcgccct ccccttcgca cccgtcgacg gcgtcccgcc gcgcgacctc
     3721 ttcgaggggc tcgatcaagc tcgtcgggca tcccggctcg cgatccgggc actcgacgac
     3781 ggcgccagcg atcgccccgc gatgctcgac gcgcttcgcc gactcggcgg gttcgaccag
     3841 cacggcgacc cgctcgaccc gccggtgtgg ctgtggcgtg ccggcccggg ttggaacgtg
     3901 gaaccagatc gtccgctgta gcgagctacc ccgggaacag tcccgccaag atgtgctcgg
     3961 tgccctcgat gtgcttgcgg atgagcttca cggcagcgcc tgaatcgccg cgcgagagca
     4021 gcgccacgag gtgccggtgc tgctcgttcg agcgcgtcag cacctcctcg gggtgagcga
     4081 tcagggcgat cagctcgccc atccgcccct gcacgtccgt catctcggcc acgagccgtg
     4141 gcgagcgcgc cgcctcggcc agcgccacgt ggaagcgcat gtccgcgcgg cggtagtcct
     4201 cgaactcgat cgcgtcggtc atcgtgtcca cgagctcgag catccggtcc agatcctcgt
     4261 ggtgcgctcg ctcggccgct agcaacacgc agccgacttc gatcgccacg cggtggtcga
     4321 gcacggactg cacgtactcg tcgtcgggct tggccgtgcc gggcacagcg agaggcggcg
     4381 agtccgcgac gaacgtgccg ccattgcggc cgcgcacgga gaccagatgg ccgctctgca
     4441 cgagcgccgt tatcgcctgc cgcagcgtgg aacgcgagat gccgagctcg agagcgaggt
     4501 cgcgctccgg cggcagctgg gacgaggcgg gaagcagccc taggcggatc gccgtgccca
     4561 gccgctccac ggtctcctca aacgtggtcg gcggcgcaac cggctcgaaa accgcctcga
     4621 gggcggtgct gcgttccatt gccaatcgtg aaggtacagg cgccgcgcct ttgatgcgac
     4681 acctcgttca ggttcagtac agctcgaggt actcctcgcg ctcccaatcg gtcacgtggt
     4741 cgtagaagcg ctgcagctcg taccgccgca tcacgccgta cgccttgacg aactcgtcac
     4801 cgaggagctc gacgatccgc tcgtcggact cgagcgcctc gaggccctcc tcgaccgtgg
     4861 tcggcagctt cggcttcgac tcgtcttcct ccgcaggcgg tgccgccggc ggttcgagag
     4921 ccagctcgtc ggtcacgccg agcaggccgg ccgccagcag ccccgcgcac gcgaggtacg
     4981 ggttcgacat cccggtgggc gcgcggttct cgatgtgccg cgactccggc gagccgccct
     5041 tgacgcgcac gaacgcgctg cggtcctcgg gcccccacga cacattcgtc gggctgaagg
     5101 tgtgggtgcg ccggcgcttc aggcagttga tcgtcggcgc gagcagcgtg tagatcgagc
     5161 gtgcgtggcg cagctggccc gcgatgaacg cgcgcccgac cggggacagg cgatactcgt
     5221 cggactcgtc ggagaacgcg ttcgcgccgc cttcgagctc gagcagacaa atgtggttgt
     5281 ggcagccgca gccggcgccg tctgcgaacg gcttcgacat gaaggtcgcg aggtagccct
     5341 ccaggtgcgc gatctccttc accccgttct tgaagctgaa cgcgtcgtcc gggccgttca
     5401 tgccgatgcc ggggccgaag ttgatctccc actgcgagcc cgcgtactcg cagttcgcgg
     5461 tgatgatgtc gagcccgaac gcgcgcgagt cgtcgacgat tcgctggatc gccggcacgt
     5521 acgtgttccg caccgtgttg aagatgtggt agccctggaa cagcggctgc ttggtctccc
     5581 cgtcgagcag gtagaactcg ggctcgaccc cgatcaaggc ctcgtaacca agctcatggc
     5641 agcggtcgag cacgcgcttg aacacctgcc gcggcgcggc gccgagtggc cgcccgtcgt
     5701 cccagaaggt gtcgcagatc atgcgcccgg tggcgtcagc ccagggcacc accttcgccg
     5761 tcgaggggtc gggcttcaga cgctggtcgg cgtacgcgac ctcctcgttg tagagcgtgc
     5821 cggggaccac gtcggaacgc gtgtcgagca ccacggtgcc gccgtacatg ttcagcccgg
     5881 accgcgcgta cgacgcggcg tggtcgatcg ggatcagctt cgagcggctc gtgccgtgga
     5941 tgtccggaag ctcgaagcgg acgttgcgga tcccctgctc acgccaccgc gcgagcacct
     6001 gctcgacagt ggtctgctcc tgcgccgtcg ccactccgac tacctcctcg taccgttgtc
     6061 ccaagtttcg acgtcgagcc cgagctcggc tgcgagctgc tcgacccgtg ccgcgtccgc
     6121 cgcgtcggtg acggcgagca cgcctccctc ccgcccgtcg ccgcgaagca gcgccgccgc
     6181 aaccgccgcg ggcggctgca gatggtgcgc ctgccagccg aaggtgggat acgagtcgca
     6241 caccacgacg agcgatccgc ccgcgccgcg cacgagcgac acgagttcga cgaagtggcc
     6301 cacgtcccat tcgtgcgagg gcggatcgac ctcctcgccg gcaaggtgcc tgagcagcgt
     6361 ctcgagcggc gggcgcgcgc cccagaagtg gccggtgcgc acgttggcga tcagccgcgc
     6421 gtcgcccgcg ccatcgacga ggcgttccac cgtttccgca ctccactcgc cacgaagcgg
     6481 cacgcagcgc agctcgccgc gcgacacctg ctcgatcgct tgagcgagat ccgccgccgc
     6541 ggtgccagac accgccggtt ccgcggccag cggcagctcg acgcggtagt cgcgcctgct
     6601 gaccgcgccg gtaggcacag acgcctgcgg atcctcgtcc ggcagcaccg tgcccgcggt
     6661 cagcgcgatc aggtcctgat cgacgtcgaa gccggcgtcg cggagcacgc gggccgcgtg
     6721 gaacggcccg cacaggttgt ccttctgctg caaagccgcg cgcacctcgc cgatcccggc
     6781 gagtgcgcgc agccctgttg cagcgtgagg gttcaacaac cccctaggtg tcgacgcctt
     6841 ggtcgatcat ccggccgacg acctggtagc gccgtgggtc ccggagcttc aggtagtagg
     6901 cgaggaggag gcccagcacg aaccacccgg cgacgatgaa cggcaccgcc ttgatgaacc
     6961 cgacgtctcc accgatctcg gtgagcttgg tgatcagcag gacgagcacg gcgatcattc
     7021 ccgccgcgcc gaggatcggc gccaaccgcg tcctccacca gtgcacgtcc tccttgtgat
     7081 agcgctcgaa gtaggagaac gacgagatcg cggtgagcga ctgcacgagc aggatcccga
     7141 acgtgccgag cacggcgagc cagccgtaga gcgccacgta tgggtccttg tcgaggatca
     7201 cgaagatcgc gacgaggatc gccgccatcg ccgactggaa cagcgaaccg gcgtacgggc
     7261 tgtgccactt cggatgcgtg cgcccgagcg cggcggggaa cacccgctca cgccccaggt
     7321 tgtagaagta gcgcgacgcc gcgttgtgga acgccatgcc gcacgcgaac gagccggtca
     7381 cgatcaccca ctgcatgatg tccttggcga gcgtgcccac gaaatcctgt gttggagtga
     7441 agaagaagtt ggtcgcgtcg ttctgagcct gcttgatcgc ctcgtcgtgg ccccagcccg
     7501 agatcaccat ccacgacgtg aacgtgtaga agatcccaag tgcgatcacg gcgatgtacg
     7561 tggcccgtgg gacgatcttc ttcggattcc tcgactcctc gccgtagttc accgtcgcct
     7621 cgaagccgac ccacgaccag aaagcgaaga agatgccgac ggcagcggcg ccgccgccga
     7681 ccgagctgaa gccctccgag gggctcagcg gcttcgcgct cagcccgtcc gggccgccgc
     7741 ccgagaagag gatgccgaag tccatgatca gcaggatcag cacctctgtg atgagcgcga
     7801 cgcccaagac tcgcgccgag atacgcacgt cgaagtacgt gagcaacgag atgatgatga
     7861 gacccgcgaa cgcgtaccag ccccagccga tgtcgatgtg gaggaagcgg ttgaacgtgt
     7921 ccttcgcgaa gaacgcgaag atgcccatca gcgaggcctc gaagatcatg tagcaggcca
     7981 tcgcggtcca acctgacgca aggccgacgg gacgacccag gccgtgcgag atgaagctgt
     8041 agaaaccgcc cgacgcggtc agtttccgcg ccatcgccac gtaacccacg gagaacaccg
     8101 tgagcacgat cgtcgcgatc cagaagccgg acggcgcgcc gatgccgttg ccgaagccga
     8161 cagcgtacgg cacgttcccc agcatcgcgg tgagcggtgc ggcgccggtc accgccagga
     8221 aaaggacgcc tacaagtccg acagcgccgg catggaggcg atggacgtca tctctttggt
     8281 tgacgtcgac cgactcgatg accagaccac cctcgtgacg gtactccacg ccgcgcggcg
     8341 gttcagccgc cgacgcacca ccgccaggtg gctgtgactc gattgtcata catcccccct
     8401 cgttggatgt ggcccgtctc cgggtcctgg tgcctcatat cgcaggttcg gctcttggtg
     8461 tcaagtctgg tcctttaggc caacgagcgt gctctcgagc gagctgcgtt cgcgaagtcg
     8521 ttgaacagac gggagcgctc gtcggcctcg ggatgccact gcactccgag cacccaccgg
     8581 ccatcgctcg cctctaccgc ctccggcagt ccgtcgatca ccgcgcgtcc gctcacgctc
     8641 agcccctcgc cgactttgtc gaggccctgg tggtgatggc agcgggccac gtggacctcc
     8701 tccccgctcg cgcgcgcgac gagcgagccg cgatcgagcc gcacctcgtg ctcggtgccc
     8761 tcgaacgacc cgagcgcctt gcggtgcggg ttcgtgccgt ccggcgctgt caggtcctgc
     8821 agcagcgtgc cgccgaacgc cacgttcatc agctgcatcc cacggcagat gccgaacacg
     8881 ggcatctcgc gctcgagcgc gccacgcagc agccgcagct cgaacgagtc gcgctcgggg
     8941 tacgtggcct ccgtccgcgg gtcgcggtcc tcgtcgtagc tctccgggtc gatgtcggcg
     9001 ccgccgatca gcaacaggcc gtcgatccgg tcgagcagcg cctcgggcgc gcgcgggtcg
     9061 atcggcagga tcagcgcgat cgcgcctgtc cgctgcacgg ccgacacgta cgagtcggcc
     9121 acgaggtggg ccggctgctc ccagaagctc cagcgggcgg tctcgcgcac ggcgcagatg
     9181 ccgatcacgg gctcccagtg cgcggtcacc agctacaccg ccctctcata gcgttcgcgc
     9241 tcccagtcgc tcacggactc gcgccatttc ttcagctccc actcgcgcga gacctcgaag
     9301 taggccgaga actcctcgcc gagcgcctcg cgcagcacgg cgtccgcccg gaaggctgcg
     9361 atcgcgctct cgagcgagcc ggggagcggt tgcagatcct cgcgctcgga cgcgtcaccc
     9421 accacgggct ccggcgggct tgcaccgcgc cggatgccgt ccgcggccgc agccgcgagc
     9481 gctgcgagcg ccaggtacgg gttcgcgtcc gcaccgggcc gcctgcactc gatgcggcag
     9541 cgctccgggt ggtcggcgga gcggatcgcc cgcacggccg ccgagcggtt ctcgagtccc
     9601 cagctcacat tgaccggcgc gaaccagccc ggcacaagcc gcttgtagga gttcacggtg
     9661 gggttcatga gcagcgacgc tcccggcagg tgatcgagca cgcccgcgac cgccgacgcg
     9721 aacacgcccc cgagctcgcc gtcgcccgaa gaggcgaacg cgttctcgcc gtcggcccag
     9781 cacgacagat gcaggtggcc gctcgagccc tcttgcccgg gcaccgtctt cgcgaggaag
     9841 gtcgccgtca gtcccatcgc ggccgccacc tccttggcgg cgagcttcac gaaggcggca
     9901 tcgtctgcgg cacgcaggcc ggggcgcgcg gcgatgttca gctcgagcag tccgggcccg
     9961 gcctcggtgt gaacggcggt caagtccacg cccagcccgt cgagcgccgg gccgagctgg
    10021 cggatgaagt cgtcgaagcg cgcgatctcc cccacgctgt agctgatccc gctcgacagg
    10081 aactcgccgt tttcatcgcg tagccggatc tcgtactcga acgccgcggt cacgtcgtag
    10141 ccgagctcag acatgtccgc gagcacgtgg cgcagcacct cgcgcgagtc gagcatgcag
    10201 gcgctgccgt ccggccacga cgggcgcgcg agacagacgc tccagccggg ccgccacgcc
    10261 atcgtccgga gcgtctcgat gtcgggctgc acgatcatgt cgcccgctcc cgagcggatg
    10321 ccgaagctct cgtagtcggt gatcggctcg tcggtcgggt cgagcgcgag cagcaggtcg
    10381 gtcatcaccg tgcccgagcg cagcgccgac tcgaacgcgg acggccgcag cgccttgccc
    10441 cgaatctgcc cgtgcatgtc ggggatgccg agcagcacga actcggcgtc gtcgggcacg
    10501 cgcgccggcg cagccggctg ggcgaacgtg gacgtgtccg cctcggtcaa gcgagcctct
    10561 ccttcacctg gtcctccacg tccgccatcg ccgcggcaaa gcggtccagc atcgcggcga
    10621 ggtcgtcgtc ggtggagttg taggccggag cgagcagcgt ctgctcgccc gtgaagccgt
    10681 ccggcgtgga gtgcgacgac gtgacgagca ccccgtgctc gaacgcggtc tggtcgacca
    10741 tctcggcggc gtcgatctcg accggcagcg gcgactcgcc gtcgcgcgga tccacgagct
    10801 ccactcccaa gaggtagccg cgcccgcgca cctccccgac gatctccgac ccgtcgagcg
    10861 cctgctcgag ctcgtcgcgg agcgaggggc cgcgctcgcg cacgcgctcg agaagcgcgt
    10921 tgtcctccag gtactcgagc accgcgagcc ccacgccgca cgggagcggc gcgccgtccc
    10981 aggtgtgccc gaggt
//
LOCUS       DASVXS010000101        12095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1300122, whole genome shotgun sequence.
ACCESSION   DASVXS010000101 DASVXS010000000
VERSION     DASVXS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300122"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1100
                     /locus_tag="VF752_05695"
     CDS             21..1100
                     /locus_tag="VF752_05695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX8855076.1"
                     /translation="MTVESPKLAAARAGLEAARAAVAKGPAPSAEALRAAYLDLLKLT
                     LCDLAGTSTTSVGRTPDGQVMAREMDGDQLRLRSAGMDWPLHGLTMVGLGRLDDLQRC
                     VEIVVRDGVEGDLIEAGSWRGGASILMRATLDTLGESGRTVWVADSFQGFPRVDDAAR
                     GDDELDVFDFLAVSLEEVKENFARFGLEDGVSFVPGFFEETMPGLAGRRWGVARLDGD
                     TYDATWLTLRTLYPDLSIGGYLIVDDFGALDECQQAVEEFRDRHGITEPLEQVDWTCV
                     RWRKERDVAVDDEATPEHRPATAPQPVERSTRAHVPTMAELDLERRVDDLEQRLAAAE
                     AEIRRLTQSPLGAAKRRLGNKLRRG"
     gene            1114..2025
                     /locus_tag="VF752_05700"
     CDS             1114..2025
                     /locus_tag="VF752_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8855077.1"
                     /translation="MIAFGSSITSPEVYRRCAEPGIRVAAEPDSEVFAHAAAGSIFRS
                     YNLLLDRAAEVEDLEALVLLHQDAEIVDPDFCKKLREALKDPDVGVVGCVGAVGVRTI
                     AWWEGSVTWASFIHRYGELGGGDLPAFSWNGRELPPYARTGEVDTVDGFVLGLSPWVV
                     KNIRFDESLGQLHGYDFDFCLQVREAGRKVVTSDFKVIHHHSLDLVSDPETWVEAHMR
                     VAEKWQDRMPGVGNAGGDWKQRARRAEAEAAAARAQVVSTQLKSDARATALQRELEQV
                     TNSTSWRVTKPLREATLKLRGARRRGS"
     gene            complement(2022..2999)
                     /locus_tag="VF752_05705"
     CDS             complement(2022..2999)
                     /locus_tag="VF752_05705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8855078.1"
                     /translation="MDRLALNIDPYANDPGHWGASLVANAELLIPCLDAAGARSVVEV
                     GAYAGDVTSLLLEWAAGSGARVWAIDPSPQDRLVKLAEEHSNLELARAPSLEALKTIP
                     PADAVIIDGDHNYYTVSQELRLIEAAAAGGPLPLLIFHDVTWPHARRDDYFSPDVIPE
                     DERQPIVEGGGLYPGVEGIRPGGLPYHYPAAREGGPRNGVLTAVEDFVNARDGMQLAV
                     VPAFFGFGVAWHRDAPYADAIAAIVEDWDGNPLLERLEANRVLHLASSHFQMVEAAKW
                     QRRAALKDELLRELLKSKSFSLAVAISRLRQRGKPAYSKDDVRRALEAD"
     gene            3069..5816
                     /locus_tag="VF752_05710"
     CDS             3069..5816
                     /locus_tag="VF752_05710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8855079.1"
                     /translation="MTAVPEEQVAREAVHRLDVELDAPLPGELAVGRGTALFVAGWCF
                     CPQAHIRALNLVVDGERQPVMEYGMPRLDVMRALHPGVDPYDTSGMDADPASEEDPLL
                     LSYRSGFWGMARFVPRERDAPYELRLEAELEGGGSATADLATLEPRQLELPVSVDEPE
                     PAGGPLVAICMATYEPPLELFRRQVESIRAQTHRNWVCVISDDCSSAEGLAALKEVVG
                     DNPRFVVSRSPRRLGFYGNFERALSLAPHAARYVAMADQDDRWLPEKLETLVAGIGDS
                     QLIYSDARVVRADGQVVSDTYWSRRRNNHSDLLSLLVANAVTGAASLLRRDLLDYALP
                     FPPAQFAHFHDHWIGLTALALGDIAFCDRALYDYVQHGDASLGHAAANRMPSLRERLG
                     ALRRDPRERVRMWRMHYFVDVCRLTQFATVLRMRCGERMSADKRRVLDRFLHTDRSLT
                     ALARLWARGARELVGRPETLGAEWMLSHAFAWRRALSATTRERPQRGLRLDSVPPPSL
                     APAPGRRVPAAPGPRAVAEKIAPLELAVSDDAPARVNLLIPTIDLEHFFGGYIAKFNL
                     ARRLAERGVRVRVVTVDPVAPLPKSWKRQIEAYSGLAGTFERVEVAFGREAQGLEVSR
                     SDRFVASTWWSAHIAANAARALGNARFLYLIQEYEPFTFPMGTYAALARQSYDFPHFG
                     LFSTELLREYFRLHGIGVYADGTAVGDRESVSFQNAITAIPAPSAGELAARQTRRLLF
                     YARPEPHAARNMFELGVLALTRTLELDAFHGDWELHGIGTLDSGRRIELTDGAALELL
                     PRSEQSEYARILGQHDVGLSLMYTPHPSLVPIEMASAGMLTVTNSFENKTPEALAAIS
                     PNLIAAEPTVDGIAAVLAEAAAGVSDFERRARGSAVQWSRDWNESFGDDLISRLTSFL
                     D"
     gene            5813..7060
                     /locus_tag="VF752_05715"
     CDS             5813..7060
                     /locus_tag="VF752_05715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8855080.1"
                     /translation="MTATDSRSIAEPPAPSPAVAPPPGSPRFALFDSLRGIAVLFIVG
                     YHVAAVTGFINNGRVGDAIIVLGNEALILFFVISGFLLYRPYVSARAKGRPQPSTRRY
                     ARRRVLRIVPAFWVALTVLAIFPGMVGIFSDDWWRYYGFLQLYSHQTLLTGIPPAWSL
                     SVEVAFYIALPFWAMAIRKVHIGSGENAWLRAELAGLALVACFGVVIQVLAARKVITD
                     LIAQSVLGACVWLALGMCLAVASVAAERRRGRTRLVDVVTQHPALCWLGAVASLVGLV
                     ALLHPGGLFGLILATRTKQEYATTLGSIALTATLSTLLVLPAVFGERAGGLPRRLLAW
                     APIAWIGLVSYGIYLWHFPVAHVLGLTKDPQHFSATGLGLARKIHHATTPILFVLTLG
                     VTAAIAAVSYHVIELPFLRRKER"
     gene            7057..8565
                     /locus_tag="VF752_05720"
     CDS             7057..8565
                     /locus_tag="VF752_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8855081.1"
                     /translation="MRPRTLIAAAGAALLLAGPVALAFYSGGFFTEPRLVAALAAWLL
                     VLLLAIAGPAPVPRAFPGWLAVSGLALLTVWSAISISWAPLRGPALESVERLLLYLGA
                     LLVGIGVLRDRRALRAVEPAVAAGAAIVIGYGLSGRLLPGLIHLAHSARAGGRLEQPI
                     TYWNAEGALAAVGFVLCARIAGDPSRRPVTRALAAAATAPLGAGIYLSYSRGAIAAAC
                     VGLVLLVALAAGRAQLRAALVALVTGVVAAACSSAFAGVASLHGSLGDRERDGALMLV
                     ILVVLAVVAALVTLGRGGLRDEPLPGGRRLAAVAFAAVGLVAVGLAVGGLQEKVSAAD
                     LATAKATRLTTVESNRYEYWRLGARAFADHPFGGLGAGGFRTYWLNHRPIKETVQNVH
                     SLELETAAELGVPGLLALGAMLTGVGLAARRALERERALAVGVTAAAVVWLLHASIDW
                     DWQLPAVTLPAITLAGALIALSERLPARRVERVEPAHGGDHPAHRRGVVAAD"
     gene            complement(8479..8853)
                     /locus_tag="VF752_05725"
     CDS             complement(8479..8853)
                     /locus_tag="VF752_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855082.1"
                     /translation="MIARVAVALVAVLAIAWLVVLERDTRLFANGKSHTGDLARAQHD
                     FKRARLLSPDTTPDLALSLLYQKHGDRSRALTAAESILRREPENLTAWAQLYSVGRND
                     PATVRRVIAAVRRLDPLDAPRR"
     gene            complement(8850..9695)
                     /locus_tag="VF752_05730"
     CDS             complement(8850..9695)
                     /locus_tag="VF752_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855083.1"
                     /translation="MSDGAFELRDVGGPSALGGGWRRSFELLYLIAVTEFKRTYFGTA
                     LGYLWSVCRPLLLFGVLLAVFTQAFHLGSNVPHYPELLLFNIVLFTFFQEATGSAVSS
                     IVSQEAIVRKTQFPRLVIPLAVVLTAMFNLVLNLLVVFVFLLSFGVDPMWTWLLLPVV
                     ILLLFVITTAVSMIVSSLYPRFRDVGIIWTVMATALFYATPVLYPIEQVSAPLRHVIA
                     FNPLAPLFELARKWVVDPNAPGPASDIAGGPVRLAAAMVIYATICVLAVWIFNREAPR
                     IAEEL"
     gene            complement(9692..10951)
                     /locus_tag="VF752_05735"
     CDS             complement(9692..10951)
                     /locus_tag="VF752_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855084.1"
                     /translation="MTELAALEERRVQVATAPVVIEARGVHKTFRIPQHRVDSLKERA
                     THPFARQEYRELQALRDISFDVHKGEFFGIVGRNGSGKSTLLKILSSIYRADAGRVRM
                     AGRLAPFIELGVGFNPELTSRENVVLNGVLMGLSQREARRRLDAVLEFAGLEEFADLK
                     LKNYSSGMMVRLAFAVMVEADADIMLIDEVLAVGDASFAQKCMDVFREKRRAGKTLVL
                     VTHDMATVQGFCDRAMMIHDGELQYIGDPEEAALRYYRINFAEADGADAGPQPGGVPD
                     VNARLVDAWLENEAGERVDNIEQGEPIGFNVVVEARQELVNPVFAFHFLNADGVQVFG
                     LNRGLEGTDRVPARERVRVSGRIENPLLPGRYFVNCWISRNRAQGDLALHVMRLLDFV
                     VYGTRPGPGSVSVESDVTAVVEKRDDR"
     gene            complement(10965..>12095)
                     /locus_tag="VF752_05740"
     CDS             complement(10965..>12095)
                     /locus_tag="VF752_05740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8855085.1"
                     /translation="RLADGREAIVPLARVPVVEPPPAPTFEVLPAGTIAICMATFEPD
                     MNLFRAQVESLRAQTYERWTCLVSDDCSSPEHFEQITEAIGADPRFVITRSEVRRGFY
                     RNFERALEMVPDGVELVALCDHDDRWYPEKLQVLHDSLGPAQLVYSDQRLVDIDGRVL
                     RDTLWRGRRNNHTDLASMLVANTITGAASLFRREVLETALPFPDPPGIDFHDHWIALV
                     ALASGDVAYVDRPLYDYVQHSGAVFGEVTQGPKPTLSGRLRGWRAAYFCGYMQRDVQA
                     QTLLARLGSRLAAPKRRVLERYVAAQRSLPAFLWLAARSLRRFAGRSETLGSETELAQ
                     GILWKWLIEARSRLPGHRLSDASFPDPMSFQQKRLRRWRARV"
BASE COUNT         1743 a         4276 c         4231 g         1845 t
ORIGIN      
        1 caatatgatc gcgccgctcc atgaccgtgg aatcgccaaa gcttgccgct gcccgcgccg
       61 gtttggaggc cgcccgcgcc gcggtcgcga agggcccggc cccgagcgct gaggcgctgc
      121 gcgccgcgta tctcgacttg ctgaagctca cgctctgcga cctcgccggc acgagcacca
      181 cgtccgtcgg gcgcacgccg gacggacagg tgatggctcg cgagatggac ggcgaccagc
      241 tgcgcctgcg gtctgcggga atggactggc cgctccacgg gctcaccatg gtcggcctcg
      301 ggcgtctgga cgatctgcag cgctgcgtcg agatcgtcgt gcgggacggc gtggagggcg
      361 acctgatcga ggccggcagc tggcgcggcg gcgcgtcgat cctcatgcgg gcaacgctcg
      421 acacactcgg cgagagcggc cgcacggtgt gggtggccga ctcgttccag ggcttcccgc
      481 gcgtagatga cgccgcgcgc ggcgacgacg agctcgacgt tttcgacttc ctcgccgtct
      541 cgctcgagga ggtgaaggag aacttcgcgc gcttcgggct ggaggacggg gtgagcttcg
      601 tgcccgggtt cttcgaggag acgatgcccg ggctcgccgg acgtcgttgg ggggtcgcgc
      661 gtctcgacgg cgacacctac gacgccactt ggctcacgct gcggacgctg tacccggacc
      721 tctcgatcgg cgggtatctg atcgtcgacg acttcggcgc gctcgacgag tgccagcagg
      781 cggtggagga gttccgtgac cgccacggga tcacggaacc gctcgagcag gtggactgga
      841 cttgcgtgcg ctggcgcaag gagcgcgacg ttgcggtgga tgacgaggcg accccggagc
      901 accgccccgc caccgcgccg cagccggtgg agcgatcaac tcgggcgcac gtccccacga
      961 tggccgagct cgacctcgag cgacgcgtgg acgacctgga gcagcgtctc gcggccgccg
     1021 aggcggagat ccgccgcctc acgcagtcgc cgctgggcgc cgcaaagcgc cgactcggaa
     1081 ataagctcag gcgaggctga gaaagacctc gccatgatcg cgttcggctc gtcaatcaca
     1141 tctccggagg tctaccggcg ctgcgccgag ccagggatcc gcgtcgcggc ggaacccgac
     1201 tcggaggtgt tcgcgcatgc cgccgcgggc tcgatcttcc gcagctacaa cctcctgctc
     1261 gaccgcgccg ccgaggttga ggacctcgag gcgctcgtac tgctccacca ggacgccgag
     1321 atcgtcgatc cggacttctg caagaagctt cgcgaggcgc tgaaggatcc cgatgtgggc
     1381 gtcgtgggtt gtgtcggcgc cgtgggcgtg cgcacgatcg cgtggtggga gggctcggtc
     1441 acctgggcgt cgttcatcca ccgctacggc gagctcggcg gcggcgacct ccccgccttc
     1501 tcgtggaacg gcagggagct tcccccctac gcgcgcacgg gcgaggtcga caccgtggac
     1561 ggctttgtgc tcggcctgtc gccctgggtg gtgaagaaca tccgcttcga cgagtcgctc
     1621 ggccagctgc acggctacga cttcgacttc tgcctccagg ttcgcgaggc cggccgcaag
     1681 gtggtgacgt ccgacttcaa ggtgatccac caccactcgc tcgacctggt gagcgacccc
     1741 gagacgtggg tcgaggcgca catgcgagtg gccgagaagt ggcaggaccg catgcccggc
     1801 gtgggaaacg ccggcggcga ctggaagcag cgcgcgcgcc gcgccgaggc ggaggcggcg
     1861 gccgcccgcg cgcaggtcgt atcgactcag ctcaagtccg acgcgcgtgc gaccgcgctg
     1921 cagcgcgagc tcgagcaggt caccaacagc acgagctggc gcgtcaccaa gccgctgcgc
     1981 gaggccacgc tgaagctccg cggagcacgg aggcgcggct cctaatcggc ttcgagggcc
     2041 cggcgcacgt cgtccttcga gtacgcgggc ttcccgcgct ggcgcagtcg cgagatggcc
     2101 accgcaagcg agaacgactt cgacttcagg agctcccgca gcagctcgtc cttcagcgcg
     2161 gccctgcgct gccacttcgc ggcctccacc atctggaagt gagacgacgc caggtggagc
     2221 acgcggttgg cctcgaggcg ctcgagcagc gggttgccgt cccagtcctc gacgatggcc
     2281 gcgatcgcgt cggcgtacgg cgcgtcgcga tgccacgcca cgccgaagcc gaagaacgcc
     2341 ggcaccacgg cgagctgcat gccgtcgcgc gcgttcacga agtcctcgac cgcggtgagg
     2401 acgccgttgc gcgggccgcc ctcgcgcgcc gccgggtagt ggtacggcag gccgccgggc
     2461 cggatgccct cgacgccggg atagaggccg ccgccctcga cgatcggctg gcgctcgtcc
     2521 tccgggatca cgtcgggcga gaagtagtcg tcgcggcggg catgaggcca ggtgacgtca
     2581 tggaagatca gcagcggcag tggcccgccg gccgcggccg cttcgatgag ccgcagttcc
     2641 tggctgaccg tgtagtagtt gtggtcgccg tcgatgatca cggcgtccgc cggtgggatc
     2701 gtcttcagcg cctcgaggct cggcgcccgc gcgagctcga ggttcgagtg ctcctccgcg
     2761 agcttcacca gccggtcctg cggcgagggg tcgatcgccc acacacgcgc gccggagccg
     2821 gcggcccact cgagcaacag gctcgtgacg tcgcccgcat aggcgccgac ctccacgacg
     2881 gatcgcgcac ccgccgcgtc gaggcacggg atcaagagct cggcgttcgc cacaagcgac
     2941 gcgccccagt ggccgggatc gttcgcgtag gggtcgatgt tgagtgccag ccggtccacg
     3001 acagcggcga gtgtaacgac agcccgcggg cgcaaatctt cacccgtgct ctcgtctagg
     3061 ttgggcctgt gacggccgtg ccggaagagc aagtcgcgcg cgaggctgtg catcggctcg
     3121 acgtcgagct cgacgctccg ctacctggtg agctcgcggt gggacgcggc accgcgctgt
     3181 tcgtggcggg ctggtgcttc tgcccgcagg cgcacatccg cgccctgaac ctggtggtcg
     3241 acggcgagcg ccagccggtg atggagtacg gcatgccgcg cctcgacgtg atgcgcgcgc
     3301 ttcaccctgg ggtcgatccc tacgacacct cggggatgga cgccgacccc gcctcggagg
     3361 aggatccgct cctactcagc tatcgcagcg gcttctgggg aatggcccgg ttcgtgccgc
     3421 gcgagcgcga cgcgccctac gagctgaggc tcgaggccga gctcgagggc gggggcagcg
     3481 cgaccgcgga cctggcaacc ctcgagccgc ggcagctgga gctcccggtt tcggttgatg
     3541 agccggaacc ggcaggcggg ccgctcgtcg cgatctgcat ggccacgtac gagccgccgc
     3601 tcgagctctt ccgccgccag gtggagtcga tccgcgcgca gacgcatcgc aattgggtct
     3661 gcgtgatcag cgacgactgc tcgagcgccg agggtctcgc cgcgctgaag gaggtcgtcg
     3721 gcgacaaccc gcgattcgtg gtctcgcgct cgccccggag gctcggcttc tacgggaact
     3781 tcgaacgcgc gctgtcgctc gccccgcacg ccgcgcgcta cgtggccatg gccgatcagg
     3841 acgatcgctg gctgccggag aagctcgaga cgctcgtggc cgggatcggc gactcacagc
     3901 tcatctacag cgacgcgcgg gtggtgcgcg cggatggcca ggtcgtctcg gacacgtact
     3961 ggagccgccg gcgcaacaac cactccgatc tgctctccct gctcgttgcg aacgccgtga
     4021 ccggagccgc gtcgctcttg cgtcgcgacc tgctcgacta cgcgctgccc ttcccgcccg
     4081 ctcagttcgc gcacttccac gaccactgga tcgggctgac ggcgctcgcg ctcggcgaca
     4141 tcgcattctg cgatcgcgcg ctgtacgact acgtgcagca cggcgacgcg tcgctcgggc
     4201 acgcggcggc gaatcgcatg ccctcgctac gcgagcgcct cggcgccctg cggcgtgatc
     4261 cgcgcgagcg cgtgcgcatg tggcgaatgc actacttcgt ggacgtctgc cggctcaccc
     4321 agttcgccac cgtgctgagg atgcgctgcg gtgagcggat gtccgcggac aagcggcgcg
     4381 tgctcgatcg gttcctccac accgaccgct cgctcacggc gctcgccagg ctgtgggcgc
     4441 gcggcgcgcg cgagctggtg ggccgtccgg agacgctcgg ggccgagtgg atgctgtcgc
     4501 acgccttcgc ctggcgccgg gcgctgtcgg ccacgacgcg cgagcgaccg cagcgtggcc
     4561 tgcgcctcga ctcagtgccg cccccgagcc tcgcgccggc gccgggtcgg cgcgtgccag
     4621 cggctccggg gccgcgggcg gtggcagaga agatcgcgcc cctcgagctg gcggtgagcg
     4681 atgacgcgcc ggcacgggtg aacctgctca tcccgacgat cgacctcgag cacttcttcg
     4741 gcggctacat cgccaagttc aacctggcgc gccgcctagc cgagcgcggc gtgcgcgtgc
     4801 gcgtggtgac cgtcgaccct gtcgcgccgc tgccgaagtc ctggaagcga cagatcgagg
     4861 cctacagcgg cctcgccgga accttcgagc gcgtggaggt ggccttcggg cgagaggcac
     4921 aggggctcga ggtgagccgg agcgaccgct tcgtcgccag tacgtggtgg agcgcccaca
     4981 tcgccgcgaa cgctgcgcgc gcgctcggga acgctcgctt cctctacctg atccaggagt
     5041 acgagccgtt cacgttcccg atgggcacct acgccgccct cgcgcggcag tcctacgact
     5101 tcccgcactt cggcctgttc tcgaccgagc tcctgcgcga gtacttccgg ctgcacggga
     5161 tcggggtgta cgccgacggc accgccgtcg gagaccgcga gtccgtgtcg ttccagaacg
     5221 cgatcaccgc cattcccgct ccgagcgcgg gcgagctggc ggcgaggcaa acgcgccggc
     5281 tgctcttcta cgcgcggccg gagccgcacg cggcgcgcaa catgttcgag ctcggagtgc
     5341 tcgcgctcac gcgcacgctg gagctcgacg ccttccacgg tgactgggag ctgcacggga
     5401 tcggcacgct cgactccgga cggcggattg agctcaccga cggggcggcg ctcgagttgc
     5461 ttccgcgttc ggagcaaagc gaatacgcgc gcatactcgg ccaacacgac gtgggccttt
     5521 cactcatgta caccccccac ccgagtctcg tcccgatcga gatggcctcg gcgggcatgc
     5581 tcaccgtgac gaactcgttc gagaacaaga cacccgaggc gctggccgcg atctcgccga
     5641 acctgatcgc cgccgagccg accgtggacg gcatcgcggc ggtgctcgca gaggcggcgg
     5701 ccggtgtgag cgacttcgag cgccgagcgc gcggcagcgc cgtccagtgg agccgcgact
     5761 ggaacgagtc gttcggcgac gacctcatct cacggctgac ctcgttcctc gattgacggc
     5821 caccgactcc cgatcgatcg ccgaaccgcc cgcgccctcg cccgcggtcg cgccgccgcc
     5881 gggaagccct cgattcgcgc tcttcgactc cctgcgcggg atcgccgtgc tgttcatcgt
     5941 gggctaccac gtcgccgccg tcacgggatt catcaacaac gggcgcgttg gcgacgcgat
     6001 catcgtgctc gggaatgagg cgctgatcct cttcttcgtg atctcgggct tcctgctcta
     6061 ccggccctac gtctccgccc gcgcgaaggg ccgcccgcag ccgtccacgc gacgctatgc
     6121 ccgacggcgg gtgctccgga tcgtgccggc gttctgggtc gcgctcacag tcctcgcgat
     6181 cttcccggga atggtcggga tcttcagcga cgattggtgg cgctactacg gcttcctcca
     6241 gctctactcg caccagacgc tgctgaccgg gatcccgccc gcgtggagcc tgtccgtgga
     6301 ggtggccttc tacatcgcgc tgcccttctg ggcgatggcc atccggaagg tgcacatcgg
     6361 gtccggcgag aacgcatggc tgcgcgccga gctcgccggg cttgcgctcg tcgcctgctt
     6421 cggcgtcgtg attcaagtgc ttgcggcgcg caaggtgatc acagatctga tcgcgcagag
     6481 cgtgctcggg gcgtgcgtat ggctcgccct cggcatgtgc ctcgcggtgg cgagcgtggc
     6541 cgccgaacgc cgccgcggcc gcacacggct cgtggacgtg gtcacccagc acccggcgct
     6601 gtgttggctc ggcgcggtcg cgtcgctcgt gggcctcgtc gctctgctgc atcccggcgg
     6661 gctgttcggc ctcatcctgg cgacgcgtac gaagcaggag tacgcgacca cgctcggctc
     6721 gatcgcgctc accgcgacac tcagcacgct gctcgtgctg cccgccgtgt tcggggagcg
     6781 cgctggcggg ttgccgcggc gcctgctcgc ctgggcgccg atcgcgtgga ttggcctcgt
     6841 gtcctacggg atctacctct ggcactttcc cgtcgcgcac gtgctcggcc tgaccaaaga
     6901 tccgcagcac ttctcggcca cgggcctcgg cctggccagg aagatccatc acgccaccac
     6961 gccgatcctg ttcgtgctga cgctgggggt taccgccgcg atcgcagccg tgagctacca
     7021 cgtaatcgag ctccccttcc tgaggcgcaa ggagcgatga ggccgcgcac gctgatcgcc
     7081 gcggccggcg ccgcgttgct gctcgcgggc ccggtcgcgc tcgccttcta ctcgggcggc
     7141 ttcttcaccg agccgcgcct ggtagcggcg ctcgccgcgt ggctgctcgt gttgctgctg
     7201 gcgatcgcag gccccgcacc cgtgccgcgt gcgtttccgg gatggctcgc cgtatcgggg
     7261 ctcgcacttc tcacggtgtg gagcgcgatc tcgatcagct gggcgccgct gcggggaccc
     7321 gcgctcgaga gcgtcgagcg gcttctgctc tatctgggcg cgctgctcgt gggcatcggc
     7381 gtgctgcgcg accggcgcgc gctccgcgcc gtcgagccgg cggtcgcggc cggagcggcg
     7441 atcgtgatcg gctacggcct gtccggtcgg ctgcttccag gcctcatcca cctcgcgcac
     7501 tccgcgcgtg cgggcgggcg cctcgagcag ccgatcacct actggaacgc cgagggcgcg
     7561 ctcgcggccg tcgggttcgt gctctgcgcc cggatcgcgg gcgacccgag ccgccgcccg
     7621 gtcacgcgcg cgcttgcggc cgccgcaaca gctccgctcg gggccggcat ctacctctcc
     7681 tactcgcgcg gcgcgatcgc ggccgcgtgt gtcgggctgg tgctgctggt cgccctcgcc
     7741 gccgggcggg cgcagctgcg cgcagcgctc gtggcgctcg tgaccggagt ggtggccgcc
     7801 gcctgctcgt cggcgttcgc aggcgttgcc tcgctgcacg gctcgctcgg cgatcgcgag
     7861 cgcgacggcg cactcatgct cgtgatcctg gtggtgctgg cggtcgtggc cgcgcttgtc
     7921 acgctcggcc gcggcgggct gcgcgacgag ccgctgcccg gcggtaggag acttgccgcg
     7981 gtcgccttcg cggcggtcgg tctcgtggca gtcggcctcg cggtgggcgg gctccaggag
     8041 aaggtgagcg ccgccgacct cgccaccgca aaggccacgc gcctgacgac cgtcgagtcg
     8101 aaccgctacg agtactggcg cctcggcgcc cgcgcgttcg ccgaccatcc cttcggcggc
     8161 ctaggcgcag gtggtttccg cacgtactgg ctgaatcatc gcccgatcaa ggagacggtg
     8221 cagaacgtgc actcgctcga gctcgagacg gcggcggagc tcggtgtccc gggcctgctc
     8281 gcgctcgggg ccatgctgac cggggttggc ctcgccgccc ggcgggcgct cgaacgagaa
     8341 cgagcgctcg cggttggcgt cacggccgcc gcggtcgtgt ggctcctgca cgcctcgatc
     8401 gactgggact ggcagctgcc ggcggtaacg ctgccggcca ttacgctggc gggcgcgctg
     8461 atcgcgctat cggagcgctt acctgcgcgg cgcgtcgagc gggtcgagcc ggcgcacggc
     8521 ggcgatcacc cggcgcaccg tcgcggggtc gttgcggccg actgagtaaa gctgggccca
     8581 cgcggtcaga ttctcgggct cgcgccgtag gatgctctcc gccgccgtca gtgcgcgcga
     8641 gcggtcgccg tgcttctggt agagcaacga cagcgcgaga tccggtgtcg tgtccgggct
     8701 gagcaggcgg gcacgcttga agtcgtgctg cgcgcgcgcg agatcccccg tatggctctt
     8761 gccgttggcg aacagccggg tgtcgcgctc gagcacgacg agccatgcga tcgcgaggac
     8821 agcgaccagc gcgacggcga cgcgcgcgat cacagttcct ccgcgatccg cggcgcctcg
     8881 cgattgaaga tccagaccgc gagcacacag atcgtcgcgt agatcaccat cgccgccgcc
     8941 aggcgcacgg gcccgcccgc gatgtccgaa gccggcccgg gcgcgttcgg gtccaccacc
     9001 cacttgcgcg cgagctcgaa cagcggagcg agcgggttga acgcgatcac atgccgcagc
     9061 ggcgccgaca cctgctcgat cggatagaga accggcgttg cgtagaagag cgcggtcgcc
     9121 atcaccgtcc agatgatgcc cacgtcgcga aagcgtgggt agagcgacga cacgatcatc
     9181 gacaccgccg tggtgatcac gaacagcagc aggatcacga ccggcaacag cagccaagtc
     9241 cacatcgggt cgacgccgaa cgagagcagg aacacgaaca ccacgagcag gttcagcacg
     9301 aggttgaaca tcgcggtgag caccaccgcg agcgggatca ccagccgcgg gaactgcgtc
     9361 ttccggacga ttgcctcctg actcacgatc gagctgacgg cgctgccggt cgcctcctgg
     9421 aagaacgtga acagcacgat gttgaacagc agcagctccg gatagtgcgg cacgttcgac
     9481 ccgaggtgga acgcctgcgt gaacaccgcg agcaagacgc cgaacagcag cagcggacgg
     9541 cagaccgacc agaggtagcc gagcgcagtg ccgaagtaag tgcgcttgaa ctcggtgacg
     9601 gcgatcaggt acagcagctc gaacgaccgg cgccagccgc cgccgagcgc ggacgggccg
     9661 cccacgtcgc gaagctcgaa ggcgccgtcg ctcaccggtc atccctcttc tccaccacgg
     9721 cggttacgtc cgactcgact gagaccgagc cgggtcccgg ccgcgtcccg tagaccacga
     9781 agtcgagcag ccgcatcacg tgcagcgcca agtccccctg cgcgcggttg cgcgagatcc
     9841 agcagttcac gaagtagcgg cccggcagca gcgggttctc gatcctgcct gagaccctga
     9901 cgcgctcgcg agcgggcacg cgatcggtgc cctcgaggcc gcgattcagc ccgaacacct
     9961 gcacgccgtc ggcgttgagg aaatggaagg cgaacaccgg gttgacgagc tcctgccgcg
    10021 cctccaccac cacgttgaag ccgatcggct cgccctgctc gatgttgtcg acgcgctcgc
    10081 ccgcctcgtt ctcgagccat gcgtccacga ggcgcgcgtt cacgtctggc acgccgcccg
    10141 gctgcgggcc ggcgtcggcg ccgtcggcct ccgcgaagtt gatgcggtag tagcgcagcg
    10201 ccgcctcctc ggggtcgccg atgtactgca gctccccgtc gtggatcatc atcgcccggt
    10261 cgcagaagcc ctgcaccgtg gccatgtcgt gcgtgacgag cacgagcgtc ttgcccgccc
    10321 ggcgcttctc gcggaaaacg tccatgcact tctgcgcgaa cgacgcgtcg cccaccgcga
    10381 gcacctcgtc gatcagcatg atgtcggcgt cggcctcgac catcaccgcg aaggcgaggc
    10441 gcaccatcat tcccgacgag tagttcttca gcttcaggtc ggcgaactcc tcgagccccg
    10501 cgaactcgag caccgcgtcg agccgccgcc gcgcctcgcg ctggctcaaa cccatcagca
    10561 cgccgttcag caccacgttc tcgcgcgagg tcagctccgg gttgaagccc acgccgagct
    10621 cgatgaacgg agcgagccgg ccggccatgc gcaccctgcc cgcgtcggcg cgatagatgc
    10681 tcgagaggat cttcagcagc gtgctcttgc ccgagccgtt gcgcccgacg atgccgaaga
    10741 actcgccctt gtggacgtcg aacgagatgt cgcgcagcgc ctgcagctcg cggtattcct
    10801 ggcgcgcgaa cgggtgcgtc gcgcgctcct tgagcgagtc cacgcgatgc tgcgggatgc
    10861 ggaacgtctt gtgcacgccg cgcgcctcga tcacgaccgg cgcggtggcg acctggacgc
    10921 gcctttcctc gagtgcggcg agctcggtca tcggcctgag gatctcagac gcgcgcccgc
    10981 cagcggcgga gccttttctg ctggaagctc atcggatccg ggaagctcgc gtcggagagc
    11041 cggtggcccg gcaaccgtga acgcgcctcg atcagccact tccagaggat gccctgagcg
    11101 agctcggttt cgctacccag cgtctcgcta cggccggcga agcggcgcag cgagcgcgcc
    11161 gcgagccaca ggaacgccgg gagcgagcgc tgagccgcca cgtagcgctc gagcacgcgc
    11221 cgcttgggcg cggcgagtct cgacccgagc cgtgcgagaa gcgtctgcgc ctgcacatcg
    11281 cgctgcatgt agccgcagaa gtaggcggcg cgccagccgc gcaggcgccc gcttagcgtc
    11341 ggcttcggtc cctgtgtgac ctcgccgaac acggcgccac tgtgctgcac gtagtcgtag
    11401 agcggccggt ccacgtaagc gacgtcgccg gacgcgagcg ccacaagcgc gatccagtgg
    11461 tcgtggaagt cgattcccgg cggatccgga aagggcagcg ccgtctccag cacctcgcgg
    11521 cggaacaggc tcgccgcgcc cgtgatcgtg ttcgccacga gcattgacgc aaggtcggtg
    11581 tggttgttgc gccggccgcg ccagagcgtg tcgcgcagca cgcgcccgtc gatgtcgacc
    11641 agtcgctgat ccgagtacac gagctgcgcc gggcccaggg agtcgtggag cacctgcagc
    11701 ttctccggat accagcggtc gtcgtggtcg cagagcgcga caagctcgac gccgtccgga
    11761 accatctcga gcgcgcgctc gaagttgcgg tagaagccgc gccgtacctc cgagcgcgtg
    11821 atcacgaagc gcggatcggc gccgatcgcc tcggttatct gctcgaagtg ctcggggctc
    11881 gagcagtcgt cgctcacgag gcaggtccag cgctcgtagg tctgcgcgcg aagcgactcg
    11941 acctgcgcgc ggaagaggtt catgtccggc tcgaacgtgg ccatgcagat cgcgatcgtg
    12001 ccggcgggca ggacctcgaa cgtgggcgcc ggcggcggct cgacgaccgg cacccgggcg
    12061 agcggcacga tcgcctcgcg gccgtccgcg aggcg
//
LOCUS       DASVXS010000102         8515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1005223, whole genome shotgun sequence.
ACCESSION   DASVXS010000102 DASVXS010000000
VERSION     DASVXS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005223"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VF752_05745"
     CDS             complement(<1..504)
                     /locus_tag="VF752_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855086.1"
                     /translation="MSVGRWLDAFRGALTVRGRLRTRILDEVESHLLEAAAAQPSLPR
                     AQAEAAATAAFGDPADVARGFAEELGVRSATRATRQAGAAVALFAAAFVVMGSNGVRH
                     AAPWIVSSPAWPLAFVGAQVAAAATLATAVRFAAGGWPAPPERTADVARGAVVASTAG
                     VLSLGALV"
     gene            complement(501..815)
                     /locus_tag="VF752_05750"
     CDS             complement(501..815)
                     /locus_tag="VF752_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8855087.1"
                     /translation="MDAQVLKGHLDSLLLAVVAAGPAHGYAIIARLRDRSGGAFDLPE
                     GTVYPALHRLEASGLLKSSWDRSDGRRRRVYAVTPKGRRRLGAQRREWQGFASAVEAV
                     LS"
     gene            864..1727
                     /locus_tag="VF752_05755"
     CDS             864..1727
                     /locus_tag="VF752_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8855088.1"
                     /translation="MPALALGLVLAAAVAHAGWNVLAKHAGGGVGFVWLYSMLGALMW
                     APVALGVALIAHPTVGLAGLAFMAGSGTLHATYFVTLQRAYADGDLSVVYPLARGLGP
                     LISTAAAIAILGERPGPLAVVGALLITASVLTLAARGAAQHPGPEPRAGLAWAVATGV
                     LIGAYTLWDKHAVDGLDQEPILYLWGGNVFWALLLAPFALRRPGELARARRFNLRSAA
                     GVALLSPVAYVLVLFALKLAPVSYVAPAREVSILFGAAMGSRLLGEGDSAWRLAAASA
                     IVLGILGLAVG"
     gene            complement(1731..2294)
                     /locus_tag="VF752_05760"
     CDS             complement(1731..2294)
                     /locus_tag="VF752_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8855089.1"
                     /translation="MIRNLVRVIVGALFFGHGTQKLFGWFGGHGPEGTGQYFENLGLK
                     PGKHNALAAGGAEAIGGVLFGLGFLTPLGAAMMSGSMLTAIRTVHLEKGPWVTEGGYE
                     YNLVLLGAAYAFTEEGPGRLSLDNGIFGRDRWGTPWAIAELVTGAIASELTIRSGRQQ
                     AQQQAGPQAATPDGAGAEREQAVPTGS"
     gene            complement(2317..4380)
                     /locus_tag="VF752_05765"
     CDS             complement(2317..4380)
                     /locus_tag="VF752_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855090.1"
                     /translation="MPPAVATAAEREVPRGLGARLVALSFLLLFTELALIRWTAANIV
                     WLVYVTNFVLLASFLGIGIGFLRAKAKRDLLPLAPVALLVLVAFVLAFPVSVTRFSGS
                     RRIEGGFGMDPLPRWLSLAIVFVLSVAVMACIGHSLARTFAQLRPLDAYRLDIAGSIG
                     GIVAFSLLSFLELPPVAWGTVAAALLLVLIGRRVRWWQVAAALGVVLLLGVQSALPHE
                     HWSPYYKITAEPRPGQPGVLTVSANNIPHQTAYPVSRLRRSHPFYFFPYRHVNGPLRD
                     VLIVGAGTGNDVAVALSQGAQHVDAVEIDPVLQRLGRQYHANRPYQDPRVTAHVDDGR
                     AYLERTNKRYDLIVFALPDSLTLLAGQSSLRLENYLFTIESIRKVREHLRPAGVFAMY
                     NYYARFLLDRYASTLLGAFGTRPCAELGNELGGRHQAVLSATAGGTPRNCATPWNGKR
                     LETATDDHPFPYLRGRAIPPFYVKALAVLLLGSLLLVRASGGPLRPMAGYIDLFFMGA
                     AFLLLETKSVVQFALLFGTTWVVNSLVFAGVLLTVYAAVEVARRVRLPRPSVLYLALL
                     AALLVAWAIPPEKLLELDVLPRFLAATAVSFTPVFLANLVFAQRFRDVGSSTAAFGAN
                     LLGAMVGGVLEYLTLITGYRVLLIVVAALYGLAFVFRDGGAARAWAARTGIAPLARAK
                     PSVTS"
     gene            4489..4809
                     /locus_tag="VF752_05770"
     CDS             4489..4809
                     /locus_tag="VF752_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855091.1"
                     /translation="MKFLVVVTPRRDAPIPPPMLAEILKAQREWLQARAADGTFECNY
                     AFPTGGGMAVVNVDSHEGLNELVYDAPAFGIVDLEAKALSDSDASMGNAIAALERAAS
                     MMPG"
     gene            4821..4949
                     /locus_tag="VF752_05775"
     CDS             4821..4949
                     /locus_tag="VF752_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855092.1"
                     /translation="MTTPSELTATRAGRQVAATYANYPEAQRAVTASQTPGSQSSR"
     gene            4967..5290
                     /locus_tag="VF752_05780"
     CDS             4967..5290
                     /locus_tag="VF752_05780"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX8855093.1"
                     /translation="MRTIEKVTGRLTNAKAAAAGAGSGAWFGLFIGLLVGLFTSGPAW
                     LGLILGGALIGAFWGAVFGFIAHSTTRGERDFASTRGIVAARYELWVADEQAARAREL
                     LGAAQ"
     gene            5341..6018
                     /locus_tag="VF752_05785"
     CDS             5341..6018
                     /locus_tag="VF752_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855094.1"
                     /translation="MARAAEGEAAPRRRVTYAEAARILATRDPVLARLVEATGLMRLS
                     RPKMSPFEALVQAIVYQQLAGPSARAIHGRLIAAMPDGVEPEALLALSDSTLRTVGLS
                     TNKVRSLRDLATKVLDGTVVVSPRGLSRQSDDEIVACLSTVRGIGPWTAQMFLIFQLR
                     RFDVWPVGDLGIRHGYGLAWGVPTPTARELDPLGDPFRPYRTVLAWYCWRAAELYAGA
                     GESALTR"
     gene            complement(6024..7487)
                     /locus_tag="VF752_05790"
     CDS             complement(6024..7487)
                     /locus_tag="VF752_05790"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX8855095.1"
                     /translation="MTTATKTPKTTAKPPKSAPEQAPAVPHLSIAERIARGKAARAEV
                     PRSEHGRFEPSSARPDPVELLESQAETRVPELVPIRYGRMLVSPFTFYRGAAMIMADD
                     LAATPRSGLLVQCCGDAHLSNFGVFASPERQLIFDLNDFDETLPGPWEWDVKRLATSM
                     LIAAVDNGFAPKDQDRVVLEAVGMYRDSMRQFAAMNNLEVWYARLDIEAALQRFGGQF
                     KQKQVKRTEKALAKARTRDSMSAFSKLTHMVDGHVEIVDESPLIVPVGKLAGDRADEV
                     FGELRELLRQYRDSLGPDRRVLLEQFELHDFARKVVGVGSVGTRAWIALLLGRDTQDP
                     LFLQMKEAEASVLEPFLGPSDFSNHGERVVHGQRLMQAASDIFLGWLHVPAGLDGKQR
                     DFYVRQLKDWKGSAEIEQMVPSGMATYGKLCGWTLARAHARSGDRIAIASYLGGSNRF
                     DRALLEFARAYAEQNERDYKAFRKAVQSGRLNAETGL"
     gene            7652..>8515
                     /locus_tag="VF752_05795"
     CDS             7652..>8515
                     /locus_tag="VF752_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855096.1"
                     /translation="MATASFLASRVLPTGGFWVALAGGVALARAAERRGARQGYGASF
                     AAMLETVAIMGPARFGIPLTQALSAPLLGRLEARGAGRLIQTLACAAIRLLHNTATTA
                     LFIFVIAGGVDTYAGTYDKLAERISLLPEGSGWALGLTAAGLVGWAAFASTVQVLVYR
                     RGLRRWPAQEAVPERPRGERDSARPRRFDPRAIAFAAALVFGVLLASTAWPVLAAVAA
                     WLTLAALASGGDRNVVPTGLLLVAALGGSALLFGVLGGLSFDLALRRAARAALLVLVA
                     TWLRAAAGAGGL"
BASE COUNT         1229 a         2968 c         3057 g         1261 t
ORIGIN      
        1 tacgagcgcg cccaacgaga ggacgcccgc agtggatgcc accacggccc cgcgtgcaac
       61 gtcggcggtg cgctcgggcg gcgccggcca gccgccggca gcgaagcgga cagcagtggc
      121 gagcgtggcg gcggcagcga cctgagctcc gacgaaggcc agcggccatg ccgggctcga
      181 gacgatccac ggcgcagcgt gacggacgcc gtttgagccc atcaccacga aggcggccgc
      241 gaacagggcc acggcggcgc cggcttggcg cgtggcgcgc gtggcggagc ggaccccgag
      301 ttcctccgcg aagccgcggg cgacgtccgc gggatcgccg aacgcggcgg tggcggccgc
      361 ttccgcctgc gcgcggggga gacttggctg cgcggctgcc gcctcgagca ggtgcgactc
      421 gacctcgtcg aggatgcgcg tgcgcagcct tccgcgaacg gtcagcgcgc cgcggaacgc
      481 gtcgagccag cggccgacgc tcacgagagc accgcctcga cggcgctggc gaagccctgc
      541 cactcgcgcc gctgggcccc gagccgacgg cggcctttcg gcgtgaccgc gtagacgcgc
      601 cggcggcgcc catcgctgcg gtcccacgag ctcttcagca ggccgctcgc ctcgagccgg
      661 tgcagcgccg ggtagaccgt gccctcgggc aggtcgaagg caccgccgct tctatcccgc
      721 aggcgcgcga tgatcgcgta gccgtgcgca gggccagcgg cgaccacggc gaggagcagg
      781 gagtcgaggt ggcccttcag gacttgggca tccatgggcg acaccatacc tagtaatccg
      841 aggcatagca agacgaggcc tgcgtgccgg cgctagcact gggactggtg ctggccgcgg
      901 ccgtggcgca cgcgggctgg aacgtgctcg ccaagcacgc gggcggggga gtgggcttcg
      961 tctggctcta ctcgatgctc ggagcgctga tgtgggcgcc agtcgcactc ggtgtcgcgc
     1021 tgatcgcgca cccgaccgtc gggctcgcgg gcctcgcctt catggcgggc tccggcacgc
     1081 tccatgccac ctacttcgtc acgctccagc gcgcctacgc cgacggcgat ctatccgtcg
     1141 tctacccgct cgcgcgcggc ctcgggccgt tgatctcgac cgccgccgcg atcgcgatcc
     1201 tgggagagcg acctggcccg ctcgcggtag tcggtgcgct attgatcaca gcctccgtgc
     1261 tcacgctcgc cgcgcgcggt gctgcgcagc acccagggcc agagccgcgc gcgggcctcg
     1321 cgtgggcggt ggcgacgggt gtgctcatag gcgcctacac cctttgggac aagcacgcgg
     1381 tcgacgggct cgaccaagag ccgatcctct acctctgggg agggaacgtc ttctgggcgc
     1441 tgctgctcgc cccattcgcg ctgcgccggc ccggcgagct cgcgcgcgcc cggcggttca
     1501 acctgcgcag cgcggccggg gtggcgctgc tgtcgccggt ggcgtacgtg ctcgtgctgt
     1561 tcgcgctgaa gctggcgccg gtgagctacg tggcgccggc gcgtgaagtg agcatcctgt
     1621 tcggcgcggc gatgggcagt cggctgctgg gcgagggcga ctccgcgtgg cggctggccg
     1681 ccgccagcgc gatcgtgctg gggatcttgg gcctggcggt gggctgaagg tcaggagccg
     1741 gtgggaaccg cctgctcgcg ctcggcgccg gccccgtcgg gggtcgccgc ctgcgggcct
     1801 gcctgctgct gcgcctgctg gcggccggac ctgatcgtca gctccgacgc aatcgctccc
     1861 gtaacgagct cggcgatcgc ccagggcgtg ccccagcggt ccctgccgaa gatgccgttg
     1921 tcgagcgaca gcctgccggg cccctcctcg gtgaacgcgt aggccgcgcc aagcagtacg
     1981 aggttgtact cgtatccgcc ctccgtgacc cagggcccct tctccaggtg cacggtccgg
     2041 atcgcggtga gcatggatcc cgacatcatc gcggccccta acggggtgag gaaaccgagc
     2101 ccgaagagca cgccgccgat ggcctcggct cctcctgccg ccagcgcatt gtgcttgccg
     2161 ggcttcaggc cgaggttctc gaagtactgc cccgtgccct ctggcccgtg cccgccgaac
     2221 cagccgaaca gcttctgtgt gccgtgaccg aagaacagcg cgccgacgat cacgcgcacg
     2281 aggtttctga tcatgccggg cgccgtaccc gccgcctcag gaggtcacac tgggttttgc
     2341 tcgtgcgagg ggcgcgatgc cggtccgtgc ggcccaggca cgagcggcgc cgccatcgcg
     2401 gaacacgaac gcgagcccgt agagcgccgc gaccacgatc agaagcacgc gatagcccgt
     2461 gatcagcgtg aggtattcga gcacgccgcc caccatcgcc ccgagcaggt tcgccccgaa
     2521 cgccgcggtc gacgatccca cgtccctgaa gcgctgcgcg aacacgaggt tggccaggaa
     2581 caccggcgtg aacgacaccg cggttgccgc caggaagcgc gggagcacgt cgagctcgag
     2641 gagcttctcc ggcgggatcg cccacgcgac gagcagcgcc gcgagcagcg cgagatagag
     2701 cacgctcggt ctcggcagcc ggacgcggcg ggccacctcg accgccgcgt agaccgtcag
     2761 cagcacgccc gcgaacacga gcgagttcac cacccacgtg gtgccgaaga ggagcgcgaa
     2821 ctgcacgacg ctcttcgtct cgagaagtag gaacgcggcg cccatgaaga agaggtcgat
     2881 gtagcccgcc atgggccgaa gcggcccgcc gctcgcccgc acgagcagca gtgagccgag
     2941 cagcagcacc gcgagcgcct tcacgtagaa gggcgggatc gcccggccgc ggaggtatgg
     3001 gaagggatgg tcgtcggtgg cggtctcgag ccgcttgccg ttccacggcg tcgcgcagtt
     3061 gcgcggagtc ccgccggcgg tcgcgctcag caccgcctgg tggcggccgc cgagctcgtt
     3121 cccgagctct gcgcagggcc gcgtgccaaa ggcacccagg agcgtgctcg cgtaacgatc
     3181 gagcaggaag cgcgcgtagt agttgtacat cgcgaacacg cccgcggggc ggaggtgctc
     3241 gcgcaccttg cggatcgact cgatcgtgaa gaggtagttc tcgagcctga ggctcgactg
     3301 gccggcgagg agcgtgagcg agtcgggcag cgcgaagacg atcaggtcgt agcgcttgtt
     3361 ggtgcgctcg agataggcgc gcccgtcgtc cacgtgcgcg gtcacgcgcg ggtcctggta
     3421 gggacggttc gcgtggtact ggcgcccgag tctctgcagc accgggtcga tctcgaccgc
     3481 gtccacgtgc tgcgcgccct ggctgagcgc caccgcgacg tcgttgcccg tgcccgcgcc
     3541 cacgatcagc acgtcgcgga gcgggccgtt cacgtgccgg taggggaaga agtagaacgg
     3601 gtgcgaccgg cgcagccgtg agaccggata ggcggtctgg tgggggatgt tgttcgccga
     3661 gacggtcagc acgcccggct gtccgggccg cggctccgcg gtgatcttgt agtagggcga
     3721 ccagtgctcg tgcggaaggg ccgattggac tccgagcagc aacaccaccc caagcgcggc
     3781 cgccacctgc caccacctca ctctccgccc gatcaggacg aggaggagcg ccgcggcgac
     3841 cgtgccccag gcgaccggtg gcagctcgag gaacgacagc agcgagaacg cgacgatgcc
     3901 gccgatgctg ccggcgatgt cgagccggta tgcgtcgagc ggccgcagct gggcgaaggt
     3961 gcgcgccagg ctgtggccga tgcaggccat cacggccacg gacagcacga agacgatggc
     4021 gaggctgagc cagcgcggaa gcgggtccat gccgaagccg ccctcgatcc gccgcgaccc
     4081 ggagaagcgc gtgaccgaca ccgggaacgc gagcacgaag gcgacgagca cgagcagcgc
     4141 gacgggggcg agcggcaaca ggtcccgctt ggccttcgcc cgcaggaagc cgatcccgat
     4201 gccgaggaag ctcgccagga gtacgaagtt cgtcacgtag acgagccaga cgatgttcgc
     4261 ggccgtccag cggatgagcg cgagctcggt gaacagcagc aggaagctca gcgcgacgag
     4321 ccgggcgccc agcccgcggg gcacttctcg ctcggcggct gtcgcaacgg ccggcggcac
     4381 tggcgcggaa gtgtctcacg cgggtcttgg cgcgcgcggc gcttgacggg cgttccgagc
     4441 gaggaagaga atcggcgagg ccctgcccag agtcgcgagg aggtcgcgat gaagttcctg
     4501 gtagttgtca ccccccgacg cgacgcgccg attcccccgc cgatgctcgc ggagatcctg
     4561 aaggctcaac gtgagtggct gcaggcgcgc gcggcggacg gcacgttcga gtgcaactac
     4621 gccttcccga ccggcggagg gatggcggtg gtcaacgtgg actcacacga ggggctcaac
     4681 gagctggtct acgacgcgcc cgcgttcgga atcgtcgacc tcgaggcgaa ggccctcagc
     4741 gattcggatg cctcgatggg taacgccatc gcggcgctcg agcgtgcggc cagcatgatg
     4801 ccgggctagc ggagcccggc atgacaaccc caagcgaact gacggccacg cgtgcggggc
     4861 gccaggtggc cgccacctac gccaactatc ccgaggcgca gcgcgcggtg accgcctctc
     4921 agacgccggg ttcccagtcg agcaggtaga gatcatcggg ctggacctga ggacgatcga
     4981 gaaggtgacc gggcgcttga ccaacgcgaa ggcggccgcc gccggcgccg gcagcggcgc
     5041 gtggttcggc ctcttcatcg gactgctcgt gggcctgttc accagcggcc ccgcgtggct
     5101 cgggctgatc ctcggcggtg cgctcatcgg cgcgttctgg ggcgcggtgt tcggattcat
     5161 cgcgcactcg accacccggg gtgagcgcga cttcgcgtcc acgcgcggca tcgtggcggc
     5221 ccgttacgag ctgtgggtgg cggacgagca agccgcccgc gcacgcgagc tgctcggcgc
     5281 cgcccagtag cgctgtcgca tttgacgccg tgcatcggcg cccgtcacga tggagcgccg
     5341 atggctcgag ccgccgaagg cgaagccgcg ccgcggcgcc gcgtgaccta cgcggaggcg
     5401 gcccgcatcc tcgcgacgcg cgaccccgtt ctcgcgcgac tggtcgaagc gaccggcctg
     5461 atgcgcctca gccgtccgaa gatgtcccct ttcgaggcgc ttgttcaggc gatcgtgtac
     5521 cagcagctcg ccggtccgtc ggccagagcg atccacggcc ggttgatcgc agcgatgccg
     5581 gacggcgtcg agcctgaggc gcttctggcc ctttccgact cgacgctgcg cacggtcggc
     5641 ctgtccacga acaaggttcg ctcgctgcgt gacctcgcga cgaaggtcct cgacgggacg
     5701 gttgtcgtgt cgccgcgtgg tctgtcccgc cagtcggacg atgagatcgt cgcctgtctg
     5761 tcgacggttc gcgggattgg tccgtggacc gcccagatgt tcctgatctt ccagctgcgg
     5821 cggttcgacg tctggccggt cggcgacctg gggatccgcc acggctacgg cctcgcgtgg
     5881 ggagtgccga cccctacggc gcgggagctc gatcccctcg gcgatccgtt ccggccctac
     5941 cgcacggtgc ttgcgtggta ctgctggcgc gcggccgagc tgtatgccgg cgcaggcgag
     6001 agcgcgctca cgcggtaggg cgcctagagc cccgtctcgg cgttcagcct gccggactgg
     6061 actgccttcc tgaaggcctt gtagtcgcgc tcattctgct ccgcgtacgc gcgggcgaac
     6121 tcgagcagcg cgcggtcgaa ccggttgctg ccgccgaggt aggacgcgat cgcgatgcgg
     6181 tcgccgctgc gggcgtgtgc acgcgcgagc gtccagccgc acagctttcc gtaggtcgcc
     6241 atgccgctcg ggaccatctg ctcgatctcc gccgagccct tccaatcctt gagctggcgg
     6301 acgtagaagt cgcgctgctt gccgtccaag ccggccggga cgtgaagcca gccgaggaag
     6361 atgtcgctgg cggcctgcat cagtcgctgg ccgtgcacca cgcgctcgcc gtggttcgag
     6421 aagtcgctcg ggccgaggaa cggctccagt accgacgcct ccgcctcctt catttgcagg
     6481 aagagcgggt cctgggtgtc gcgaccgagt agcagagcga tccacgcacg cgtgcccacg
     6541 cttcccacgc cgacgacctt gcgcgcgaag tcgtggagct cgaattgctc gagaagcacc
     6601 cggcggtcgg ggccgagtga gtcgcgatat tggcgcagga gttcgcgcag ctcaccgaag
     6661 acctcgtcgg cccggtctcc ggccagcttc ccgacaggca cgatcagcgg cgactcgtcg
     6721 acgatctcga catgtccgtc gaccatgtgg gtgagcttgg agaacgccga catgctgtcg
     6781 cgagttcggg ccttggcgag cgccttctcg gttcgcttca cctgcttctg cttgaactgc
     6841 ccgccgaagc gctgcagcgc agcctcgata tcgagccgcg cgtaccacac ctcgaggttg
     6901 ttcatagcgg cgaactgccg catcgagtcc ctgtacatgc cgaccgcctc gaggacaacg
     6961 cggtcctggt ccttcggagc gaatccattg tcgaccgccg cgatcagcat gctcgtggcg
     7021 agccgcttca catcccactc ccacggcccc ggcaacgttt cgtcgaagtc gttgaggtcg
     7081 aagatcagct ggcgctcggg agaggcgaac acgccgaagt tcgacaggtg agcgtcgccg
     7141 cagcactgca cgagcaggcc cgagcgcggc gtcgccgcga gatcgtcggc catgatcatt
     7201 gccgcgccgc ggtagaaggt gaacggcgag accagcatgc gcccgtatct gatcggcacg
     7261 agctcgggca cgcgggtctc cgcctggctc tccagcaact cgacgggatc cggccgggcc
     7321 gaactgggct cgaagcggcc gtgctcggag cgcggcacct cggcgcgcgc cgccttaccg
     7381 cgcgcgatgc gctcggcgat cgacaggtgc gggacggcgg gcgcctgctc gggcgcggac
     7441 ttggggggct tcgccgtcgt ctttggcgtt ttcgtcgcag tggtcatgcc cgcagtctga
     7501 gataccgctt cggcccagtc ctcacccctt cggggtgaat acccatgagg gcaccctggg
     7561 tggtcgccgc tacgtcaagc ttctcgggat ggcagcccgc accgacgagc tcacgccacg
     7621 tcctggctta accgcctcag gactcgccca tgtggccaca gcatctttcc tcgcctcccg
     7681 cgtcctgccg acgggcggct tctgggtcgc cctcgccggg ggagtcgccc tcgcacgcgc
     7741 tgccgagcgg cgcggggcgc ggcagggata cggtgcgagc ttcgcggcga tgctcgagac
     7801 ggtcgcgatc atgggtccgg cccggttcgg cattccgctg acacaggcgc tgagcgcccc
     7861 cttgctcggg cgcctcgagg ctcgaggtgc cggacgcctg atccagacgc tcgcgtgcgc
     7921 cgcgattcgt ctgctacaca acacggccac aaccgccctc ttcatcttcg tgatagcggg
     7981 cggggtcgac acgtacgcgg gcacgtacga caagctcgcc gagcgaatct cgctcttgcc
     8041 cgagggctcc gggtgggcgc tcgggctcac cgccgcgggg ctcgtcggct gggcggcgtt
     8101 cgcgagcacc gtgcaggtgc tcgtctaccg ccgcggcctc cggaggtggc cggcgcagga
     8161 ggctgtccct gagcggccgc gcggcgagcg tgacagcgcg cgcccgcggc gcttcgaccc
     8221 gcgtgccatc gcgttcgccg ccgcgctggt cttcggcgtc ttgctggcga gcaccgcctg
     8281 gccggtgctc gccgccgtag ccgcctggct cacgctggcc gcgctcgcat cgggcggcga
     8341 ccgcaacgtg gttccgaccg ggctgctcct ggtcgccgcg ctcggaggca gtgccctgtt
     8401 gttcggcgtc cttggtggcc tgagcttcga cctcgcgctg cgtcgcgccg cccgcgcggc
     8461 cctcctcgtg cttgtcgcca cgtggctgcg ggcagcggcg ggcgccggcg gcctg
//
LOCUS       DASVXS010000103        23603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1217526, whole genome shotgun sequence.
ACCESSION   DASVXS010000103 DASVXS010000000
VERSION     DASVXS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 23603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23603
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217526"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VF752_05800"
     CDS             complement(<1..941)
                     /locus_tag="VF752_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8855097.1"
                     /translation="MATTEATIEVKNFINGEEVPAAGGTEPVLNPATGEQLGTMPVSN
                     AEDVDAAVRAAGGAFREWSDKTPGERAEALLKLADALEEHGDELARLESLDVGKPIES
                     MKADEIPVLVDNLRFFAGAARNMEGKAADEYVENRTSMIRREPLGVVGQIAPWNYPLM
                     MAIWKIGPALATGNTVVLKPSENTPLSTARLAEIAAEFLPKGVLNVVHGHGQPAGEAI
                     VKHPDVRLVSITGSVESGKKVAAAAAEQLKRVHLELGGKAPVLIFDDADIEAAIEGVK
                     IGGYFNSGQDCTAASRVIAGPKVYDDFVSGLSDAAKSL"
     gene            complement(952..2088)
                     /gene="ald"
                     /locus_tag="VF752_05805"
     CDS             complement(952..2088)
                     /gene="ald"
                     /locus_tag="VF752_05805"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX8855098.1"
                     /translation="MKVGVPTEIKADEYRVAITPAGVRELLEHGHEVLIQQGAGDGSV
                     IPDAEYEEQGAQIVPDAASVFDGADMIVKVKEPQADEVAMLRPEHVLFTYLHLAPAPA
                     LTQALVESGATCIAYETVEDSHGRLPLLAPMSEVAGKVATQAGAFMLEKPLGGRGVLL
                     GGVPGVPAATVLVIGGGVVGMQAAFIAIGMEAEVYVFDRNLDRLRELDVAFNGRASTI
                     YSSTLAVEQMLPRADLVIGAVLIHGARAPWVIKRDQLKLMKQRAVLVDVSIDQGGCFE
                     TSRPTTHSDPTYEVDGITHYCVTNMPGAVPVTSTFALTNATLPYVVTLADNGVRAAIA
                     RDPGLRSGVNVAAGRVTHPAVAEGVGVPYTPVEEVIGSSTPQTA"
     gene            complement(2122..3693)
                     /locus_tag="VF752_05810"
     CDS             complement(2122..3693)
                     /locus_tag="VF752_05810"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEX8855099.1"
                     /translation="MLTVQRLVGEMGLELVAGEGGAEAPVRWVHITELTDPTPWLSGG
                     ELLLTTGIQLDTPERQQEFIERLVDHHLSGLGFGTGFDHKDVPEALLERARELDFPVF
                     EVPYRLPFIAITEKAFTQLVNEQYEVLQRGIAIHKRLERLVLEERGLDELVRALAAAI
                     GGTALVLSARGETIASGAFRRGMPEQAVELVREEVERRSSGTGSNGSAAAPFVPGDAD
                     LAGRSLALPVTIRAGSSPQAWLVAVRDAGGLGDFERLILQQAVTVVALELMRQRVMRD
                     TERRLAGDLLAEALAGRLDSSELATRLRPFGVGETAAVLVFSVDDPAAAEPLLDRHLG
                     GAGVGALVAMRDRLLCAVIDSREGDAEPHELAAAARVALAAEAGDVRAAASRPSPVGA
                     LRRSFHEARCALEAAALNEAPMDHVGSYRDLGAFQLLLSLQDEEALRLYCDNLLGPLE
                     NGGGDYGDELLRSLEAFIEENGQWERAARQLFCHRHTLRYRIRRIEELTGRDLSRARD
                     RIEFWLALRGRELVG"
     gene            complement(3716..5113)
                     /locus_tag="VF752_05815"
     CDS             complement(3716..5113)
                     /locus_tag="VF752_05815"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8855100.1"
                     /translation="MATRLEGAPQTAPAEHTELQDLARQHLWMHFTRMGSYTDHEIPI
                     LVKGDGCYVWDEHGNRYLDGLSALFCVNAGHGRTELGDAAAEQTRDLAFYTNWTFAHP
                     RAIELAAKVAELAPGDLNRVFFTSGGSEAVESALKLSRQYHKLRGEAGRYKVIARKTA
                     YHGTTLGCLMATGITGIRSPYEPLPAGGCHVTNTNRYRAPEGQDPLWPAEEIEQRIEF
                     EGPETVSAVILEPVQNSGGCFTPPDGYFQRVREICDKYGVVMISDEVICSWGRLGYWF
                     GAQRYDFVPDLITTAKGITSAYSPMGAVIASDKIAEPFMDDTKMFTHGITFGGHPVAA
                     AVALKNIEIFENEDLNGRVRQYEPDFKAMLDRLADLPIVGDVRGAGYFWALELVKDKE
                     TKQSFSHEEAEELLRGYLSGELYKRGLICRADDRGDPVIQLAPPLIAGPEQFDEIEAV
                     LGPVLSEAWDRIDHK"
     gene            5314..6588
                     /locus_tag="VF752_05820"
     CDS             5314..6588
                     /locus_tag="VF752_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855101.1"
                     /translation="MARSLAEEEEKTQEVTASSDPRISGGEMPSVRLQNVTKRFGDVV
                     AVDDMSVDIARGEFFTMLGPSGCGKTTTLRMVAGFEEPTAGAILVDGRHMEGVPAFRR
                     PTNTVFQSYALFPHLSVEENVAFGLRRKGVDKKEVTRRVGEELELVGLAGERKRKPRQ
                     LSGGQQQRVALARALVNRPAVLLLDEPLGALDLKLRKGLQVELKRIQREVGITFVYVT
                     HDQEEALTMSDRIAVMNRGIVEQVDQPEAVYDRPRTTFVAGFIGVSNLMPGAVQSANG
                     HDAEVRLDSGVTVTAPTDGLRSGERVHAVVRPEKLQLDPAETPSVDDRPNVEGLLESS
                     LYLGTATQVVVRLPDGVSMTVLVPNTDEEARRRLPGAGAQVRLSWASEHMHVVREEGT
                     PVHANGAMDGDGAALPDAIPTPAEPALTEEGK"
     gene            6585..7790
                     /locus_tag="VF752_05825"
     CDS             6585..7790
                     /locus_tag="VF752_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8855102.1"
                     /translation="MTDIDRAIDELIRQEVTRRRLLRNAGAGALGVSAAAFLAACGGG
                     GIEGGSNKKESQVIPKGKIATTLNFSNWPLYIDVSGKRHPTLDEFQKKYGTKVKYTEE
                     INDNTEFFGKVRQQYAAGNSGGRDLQVVTDWMAAKMIQLGYVQKLDKSAIPNVVNNMT
                     DVLKNAPIDPKRDYTAPWQSGQTGLIYRKDKVGTPQSVNDIFDPKLKGKVTMLTEMRD
                     TVGLVMLGMGKDPQSFTNMDDALAAIDKIDKANKDGQIRRFTGNDYLKDLPKGDSWLV
                     FGWSGDAVQLESDNKNIKFQQPDEGFMLWTDNMQVPVGAPHAYTAEVYMNYVYDPEVQ
                     AKITAYVNYVPPVKGVQEIIKKDDPKLAESPLVFPDAQVLSRAKQFKNLTPDQENTLN
                     TAFQKVIGA"
     gene            7806..8678
                     /locus_tag="VF752_05830"
     CDS             7806..8678
                     /locus_tag="VF752_05830"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855103.1"
                     /translation="MSALLQRRKGVLPYLLLAPGLAWLVIFFLIPLYYMARISLETGS
                     VFSGYSFSWHFATYGDALSTYWPQLERSLIYALATTGISLLIAYPLAYAIATRGGRWR
                     NALLLMVILPFFVTYLVRTLAWETILDDSSFVVNALKTLQIVPSDGRVLATTGSVIAG
                     LTYNFLPFMILPLYASLERIDKRMLEAGYDLYGRRRDVFLRVTFPLSLPGVVAGVLLT
                     FIPAAGDYVNATLLGSPRQFMIGNVIQSRYLELTDYPTAAALSFVLMAIILVLVAAWA
                     KIAGTESLMGSEAR"
     gene            8675..9568
                     /locus_tag="VF752_05835"
     CDS             8675..9568
                     /locus_tag="VF752_05835"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855104.1"
                     /translation="MTGATVAQQPAPAVPDARRRDLLKAGRWLLNQSLNLYAALAIVY
                     LLAPIAVVIWFSFNDTHSRFNFVWQGFTLRNWKDPFGVPGLLDALKVSLEIAALSTII
                     ATVLGTMMALALVRYQFRARGATNFFIFLPLATPEIVLGASLLSLFLSLGVNTGFATI
                     LIAHVMFNISFVVVTVRSRLIGFDRSLEEAAQDLGANSLQTFMRVTLPLIAPGVLAAA
                     LLAFALSVDDFVITNFNSGATVTYPLFIWGAARVSIPPQVNIVATMMFLVTVAAAILT
                     VLQQRRAEKIASVRPEQEAVA"
     gene            9568..10629
                     /locus_tag="VF752_05840"
     CDS             9568..10629
                     /locus_tag="VF752_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855105.1"
                     /translation="MSLWERVAGLPIEVESYALEGLERDVSSDFTRLSTVIHMSGGGE
                     EGLGEDVTYDGMDHIALQDAGPVHDLSGVRSFGEFCELVGSLDLFPAEPVREVSRLYR
                     RWAFESAALDLALRQAGRSLADVVERQPRPVNFVVSLRLGDPPTMEPIRKRLDLYPEL
                     RFKLDPVQGWDDGLISDLVATGAVDSVDLKGFYKGTVVDNPGDAGLYSRVIEAFPAAW
                     VEDPDVNEETRPILEPIRDRITWDAPIHSVADIEELEWKPRMVNIKPSRLGSVQELFT
                     AYDYCDANGIGAYGGGQFELGVGRGHIQYLASLFHPDTPNDTAPGGYNSTNPEPGLPS
                     SPLAPAIAPAGFRWEAPVA"
     gene            10655..11350
                     /locus_tag="VF752_05845"
     CDS             10655..11350
                     /locus_tag="VF752_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEX8855106.1"
                     /translation="MRRVRQQLGDELVGVILAGSYATGEALDVSDVDVAVVCERELDP
                     VRKRALVAALRHDALPGPARKLELAVYRRDVVESPGQTVPFELNLNSGRYEEHVSYDP
                     AEESPHWFVLDLAIARGQGRALYGPKPSELIGEVDPDAVRAALLQALDWWQRELPDSP
                     NTLLSACRAWRWAEQGDWLSKVAAGEWAMTRSAHPEAIAAAIERRPLREPVEAALVAA
                     VSDEAREALGRRR"
     gene            complement(11398..12537)
                     /locus_tag="VF752_05850"
     CDS             complement(11398..12537)
                     /locus_tag="VF752_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855107.1"
                     /translation="MATELLVIGAGPYGLSTAAFAREHGIETLILGQPMSFWREHMPA
                     GMFLRSGPDWHIDAAGVHTLEAYLEDRKIATEDVDPIPIRTFLDYCEWFRQAKGLDVR
                     EELVAELAESNGGFEATLDGGERIEAFAVVCAPGIRHYANVPDWAASVPAGRAAHTCD
                     LVRFDELAGARVLIVGGRQSAYEWAALIREHGAERIDLVHRHDVPRFEKVSWKFVDAY
                     VENTANVPGYWRNLPKAEQDAIARKFWEVGRLTLEHWLTPRLDWHGIHRWPGTEVVEV
                     TTRGADGELRVLLSNSERLAIDRVIFASGYRADLTRVPYLATLAGRITLADGFPVLDE
                     SFATNLAGLYVTGFSATRDFGPFFGFVKGSPAAATLIVRDILSRN"
     gene            complement(12605..13762)
                     /locus_tag="VF752_05855"
     CDS             complement(12605..13762)
                     /locus_tag="VF752_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8855108.1"
                     /translation="MAATADMTYALNDEQIAFRDLVRQIAQERVAPRAAEIDEKAEYP
                     HDLRKLLAEQDILGLPFDEEHGGTGTGTLTLNIAVEELSKACASTALILMIQELGSLP
                     IKLFGTDEQKQRWLPKLASGEWSPAFALSEVEAGSDPGGMRTRAMRDGDEWVINGEKN
                     WITNLGIADLFVVFAVTDPDEGHSRGISAFVVEADRPGVSIGKLEHKMGIRGSPTGQP
                     VFSDVRVPAENLIGTVNEGFKVAMATLDRSRLGVAAQAVGIAQGATDYAAAYARERRQ
                     FGKPINSFQAIQFKLADMETRTAAARELLYKACAKVDRREPDMGKYSAMAKLFASDTA
                     MDVTVEAVQVLGGYGFVKEYPVERFMRDAKITQIYEGTNEIQRLVVARTLR"
     gene            13781..14770
                     /locus_tag="VF752_05860"
     CDS             13781..14770
                     /locus_tag="VF752_05860"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX8855109.1"
                     /translation="MTPPGPTSAPVLVAPDSFKGSFSAAEVAVALARGLEAGGLPATE
                     LPVADGGEGTMDVLVGALGGEYRTARVSDPLGRPVAARYALLDDGSAVVEMAQASGLG
                     LVDESDRDAFAASTRGTGELIAAAAEAGAASVLVTVGGSATTDGGAGALAALDEAGIK
                     PKLEVLCDVRTPFELAPRVFAPQKGADPATVKRLERRLGRIAREAPRDPRGVPMTGAA
                     GGLSGGLWAYRGAKLLPGAAYLLDAIGFDDAMRASRFVVTGEGRLDEQTLEGKVVGEV
                     ATRCRQGGVACHAVVGNDSLDEFLERVIDLASVREATTLAEIEAAGRSLASFS"
     gene            14829..16157
                     /locus_tag="VF752_05865"
     CDS             14829..16157
                     /locus_tag="VF752_05865"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX8855110.1"
                     /translation="MTSNAATRKLRGSRALTLRMPELRHALAALSLAGIVVTGAVLAA
                     GSSPHRSFLLPVSRGRAPEAITGPLAEGFGLTGEQFLLFLVLMGGFYGAFLVWGRHLS
                     ARWTMSAIGVLVVLFAVAPPILSKDIFSYVAYARMGVDHGVDPYLHGPAIAPHDPVFA
                     FVGWRHAPTAYGPLFTLGSYALAPLGVAGAMWMLKAIAAASALGTCALIWRCAGQRRL
                     AQAPAVALFGLNPMVLVYAVGGGHNDLLLLLLLTGGVALCLNERPAAGAAGAVAATLT
                     KATAAVTLPFFLLGAADRRRGLIGGLAAAVAGLAVATVAFPDHALGVLRVLRHESLFV
                     SAESVPTELSRLFGAETVTPAAHLIAALALLAGLAYLVVRVWRGMDWIAGAGWALALT
                     LVTSSWLLGWYTIWTLPFAALARDRRLKIVALAICAYYVLGRWNIFIGAG"
     gene            complement(16141..16443)
                     /locus_tag="VF752_05870"
     CDS             complement(16141..16443)
                     /locus_tag="VF752_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855111.1"
                     /translation="MLINALRDVHGARTVRTEKVAFRQWLARHGELHHVGKSVVRLGT
                     RENMVCAPLLVHHRPRRDHLKLCALTTKGPHVRVLRVTTRRKLPSQLVPAALTPRL"
     gene            complement(16484..17569)
                     /locus_tag="VF752_05875"
     CDS             complement(16484..17569)
                     /locus_tag="VF752_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HEX8855112.1"
                     /translation="MRLNSSARRAALAGLLAVVALLCLAATLDERRAVADPDATAAAR
                     ARASYAAMQHRLDHTRAHAWPFVRLVAAAQSIAELPDATRRERAEARMRLRQLRRFWR
                     SGAYESLPRPPFGPGGDLYYDDNEWIGLELMRRYAVTRQRGLLERSRAILRLVIRGWD
                     RRAPTCPGGVKWTPVPRIYQRGTVTNGPGAQLAVAIYEATGDRRALGWGQRMYAWAER
                     CMLDEQAGLYQDHFRADGSRDTTIWSYNQGSMIGAAIALYRATGDRAYLDTAAARAAR
                     ALDYFEDSRLPSEPPYFLAIYFRNLLAYEQLAGGTSFRTQLERYADQAWNQSRDPHTG
                     IFGSERPEKRLLTQAGMVEIYALVAGS"
     gene            17710..19071
                     /locus_tag="VF752_05880"
     CDS             17710..19071
                     /locus_tag="VF752_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855113.1"
                     /translation="MRIGIASTLTSAALVLVLAGPASADVKSVTMKYGPIDIGPYQAL
                     RSSTYVPTPELDGFVTEMDAHVIDAETGQEIPQYQVMLHHVAFTDEARRDGSCPRKNI
                     GERFYGTSEELRRMTLPAGYGYRIGARDKWRMIWMLMNHLKTDGKVFIEYHVTVDTSP
                     SLTPVRPYWISVVPCVNDPQYSVPGGATAGATSSRKVTWSVPVSGRIVAMGGHLHGGS
                     RSLTLTQPRCGHRVLHNALPTYAPPGDPLYTVVPLLHEPDPVNVSWWQSATGWNVQKG
                     EMLRLSANYDGHRPHMRVMGIMHVYIAEDEGVGASACGAKPADAEVLGGSAVAGGRLA
                     PPSVVVPFEAAGRDGVARPISRSPGALRRYDGDANVTVADFRYSVANLSVPRGATVRW
                     HFRDSAAHDATLVNGPRGFGTPTVRKGHVESQRFTVPGVYRIYCSIHPVYMSQVVRVR
                     SKR"
     gene            19076..19594
                     /locus_tag="VF752_05885"
     CDS             19076..19594
                     /locus_tag="VF752_05885"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8855114.1"
                     /translation="MVRVRAPRLDDQAAFLAAVERSRASHRPWSFPPDSPQLYREYVT
                     KIAADDCEGFLIETGDGDLAGFATISQIVRAPLLSGYLGYTGFEPYMGQGIMRRGLIQ
                     ILDHAFGSMGLHRIEANVQPGNERSIALVQRLGFRREGFSPRYLMVGGVWRDHERYAI
                     TAEEWPPPGVGP"
     gene            complement(19750..21375)
                     /gene="aceB"
                     /locus_tag="VF752_05890"
     CDS             complement(19750..21375)
                     /gene="aceB"
                     /locus_tag="VF752_05890"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX8855115.1"
                     /translation="MARVEVKGLEIHAPVKGRMEEVLTPDALEFLSGLHRKFEDTRQE
                     LLHRRAERMSDLPAGGSLDFLDSTREIREGDWRVAPPPPDLQNRRVEITGPTERKMVI
                     NALNSGARGFMADFEDSNSPLWRNMIEGQVNLTDAIDRTIEFTQDDGKEYRLNDEVAT
                     LLVRPRGWHLPEKHMSVDGEPIAGALVDFGFFFFHNARRLLERGSGPYFYLPKMESHL
                     EARLWNEVFNHAQDELGIERGTIKATVLIETVPAAFEMDEILYELRDHSAGLNAGRWD
                     YMFSMIKCFRDRPEFLLPDRNSVTMTVPFMRAYTELLVKTCHRRGAHAMGGMAAFIPS
                     RRDPEANERALAKLREDKRREAGDGFDGTWVAHPDSVPAATEEFDRVLGERQNQVDRQ
                     RDEVEVSAADLLNVQATPGDITDEGLRNDVNVGIQYISSWLRGNGAAGIYGLMEDAAT
                     AEIARSQVWQWVRHGAELSDGRKVNPELVHRLEHQELERIRGEIGDDEWFENQGRPEA
                     SRTLFEQVALAEDFPEFLTLPAYDYLLSEIERF"
     gene            complement(21362..22636)
                     /locus_tag="VF752_05895"
     CDS             complement(21362..22636)
                     /locus_tag="VF752_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX8855116.1"
                     /translation="MASFDTIRPPAQELIDECVHCGFCLPTCPTYEVWGEEMDSPRGR
                     IVLMSEAVVGSEISPEMVMHWDRCLGCMACVTACPSGVKYDKLIEDTRAEVERNYSRA
                     PGDRLWRSMLFELFPHPGRLRVAAPLLALSRKLGMHRLARRGGPFPRLRALASLTPDT
                     PLRKTMKRLPERLQARGERRATVGFLQGCVQRVFFHDVNAATAEVLAAEGYEVHAPRL
                     PRCCGALQMHTGYDSRDLARATINAFEHCDYVVANAAGCGSSMKDYAHLLRDDPEWSE
                     RAHNFSGKVRDVTELLAEEPPRARRHPVPAKVAYHDACHLAHAQAVRAQPRELLGQIP
                     ELELVEPAGWEFCCGSAGIYNLLNPEPARELGRRKVENLRATGAELIAAANPGCAIQI
                     TATSEQLGEPLRVVHPIELIHMSIQGGSNGTR"
     gene            complement(22636..>23603)
                     /locus_tag="VF752_05900"
     CDS             complement(22636..>23603)
                     /locus_tag="VF752_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8855117.1"
                     /translation="LDTLVEHNEGDLTAILEAGVPLGRAQQTFAAAGQMLALDPPLGE
                     GAAATIGGVIASGDSGPLRHRYGAPRDLVLGVKVALSDSTVAKSGSKVIKNVAGYDLA
                     KLFSGSFGTLGLVLQVAVRLHPRPSETVTAVGSASDPEAVARGASDLAHASLEKECVD
                     VRWGHGEGAALARLGGATAHAQAEAAARLLADAGLDTEIVEDDDGIWEAQRAGQRAAE
                     QAVVRVSGLQTELGRTMRAADGLGGSLVGRGSLGISWVTVAPERVTKLREQLAPYPCV
                     VLDAPRELRASLDVWDAESSELARRVKQRFDPAGVFASGTFAGGI"
BASE COUNT         3532 a         8181 c         8140 g         3750 t
ORIGIN      
        1 agcgacttgg ccgcgtcgga gagccccgac acgaagtcgt cgtacacctt cggccccgcg
       61 atcacccggg aggcggcggt gcagtcctgg ccggagttga agtagccgcc gatcttgaca
      121 ccctcgatcg ccgcctcgat gtcggcgtcg tcgaagatca acaccggcgc cttgcccccg
      181 agctcgaggt ggacgcgctt gagctgttcg gccgcggccg ccgccacttt cttgccgctc
      241 tcgacggagc cggtgatcga cacgaggcgc acatctgggt gcttcacgat cgcctcgccc
      301 gcaggctgac cgtggccgtg gaccacgttc aggacaccct tgggcagaaa ctcggcagcg
      361 atctcggcga gcctcgccgt cgaaagcggc gtgttctccg acggcttcag caccacggtg
      421 ttgccggtcg cgagcgccgg gccgatcttc cagatggcca tcatcagcgg gtagttccac
      481 ggggcgatct gccccaccac gccgagcggc tcacgccgga tcatggaggt gcggttctcg
      541 acgtactcgt cggccgcctt gccctccatg ttgcgtgccg cgccggcgaa gaagcgcagg
      601 ttgtccacca gcacggggat ctcgtcggcc ttcatcgact cgatcggctt ccccacgtcg
      661 agcgactcga gccgcgccag ctcatcgccg tgctcctcga gcgcatcggc cagcttcagc
      721 agcgcctcgg cgcgctcgcc cggtgtcttg tcggaccact cgcggaaggc gccgccggcc
      781 gcgcgcacgg cggcgtcgac gtcctcggcg ttggataccg gcatcgtgcc gagctgctct
      841 ccggtcgccg ggttcagcac cggctcggtg ccgccggcgg cgggcacctc ctcgccgttg
      901 atgaagttct tgacctcgat ggtcgcttcg gtcgtcgcca tgttctcctc ctcaggcggt
      961 ctgcggggtg gaggacccga tgacttcctc cacgggggtg tacgggactc cgactccttc
     1021 ggcaacggcc gggtgcgtga cgcggccggc cgcgacgtta acgccgctgc gaagcccggg
     1081 gtcgcgcgcg atcgcggcgc gcacgccgtt gtcggcgagc gtgaccacgt agggcagcgt
     1141 ggcgttggtg agcgcgaacg tggaagtcac aggcaccgcg cccggcatgt tcgtcacgca
     1201 gtagtgcgtg atgccgtcga cctcgtaggt cgggtcggag tgggtggtgg ggcgcgaggt
     1261 ctcgaagcag ccgccctggt cgatcgacac gtccacgagc acggcgcgct gtttcatcag
     1321 cttgagctgg tcgcgcttga tcacccacgg cgcccgggcc ccgtggatca gcacggcgcc
     1381 gatcacgaga tccgcgcgcg gcagcatctg ctcgaccgcg agcgtggacg agtagatggt
     1441 ggaggcgcgc ccattgaacg ccacgtcgag ctcgcgaagg cggtcgaggt tgcggtcgaa
     1501 cacgtacacc tcggcctcca taccgatcgc gatgaaggcg gcctgcatgc ccacgacgcc
     1561 cccgccgatc acgagcacgg tggccgcggg cacgccgggc acgccgccga gcagcacgcc
     1621 gcggccgccg agcggcttct ccagcatgaa tgcgccggcc tgggtggcca ccttgccggc
     1681 cacctcgctc atcggtgcga gcagcggcag gcgcccgtgt gagtcctcga ccgtctcgta
     1741 cgcgatgcac gtggcgccgg actccaccag cgcctgcgta agcgcgggcg cgggcgcgag
     1801 gtgcaggtag gtgaagagca cgtgctccgg ccggagcatc gcgacctcgt cggcctgcgg
     1861 ctccttcacc ttcacgatca tgtccgcgcc gtcgaagacc gaagccgcgt ccgggacgat
     1921 ctgagcgccc tgctcctcgt actcggcgtc gggtatcacg ctgccgtcgc ccgcgccttg
     1981 ctggatcagg acctcgtggc cgtgttccag aagctcacgc acgcccgcgg gcgtgatcgc
     2041 cacgcggtac tcgtcggcct tgatctcggt cgggactcca accttcatgc gttcagtgcc
     2101 tcctcggaat cgaagcctgg ttcaacctac gagctccctg ccgcgcaggg cgagccagaa
     2161 ctcgattcgg tcgcgcgccc gagacaggtc gcgtccggtg agctcctcga tgcggcgaat
     2221 gcgatagcga agcgtgtgac ggtggcagaa cagctgccgc gccgcgcgct cccactgccc
     2281 gttctcttcg atgaaggcct cgagtgagcg aagcagctcg tcgccatagt cgccgccgcc
     2341 gttctcgagc gggccgagga ggttgtcgca gtagaggcgc agggcctcct cgtcctggag
     2401 cgagagcagg agctggaagg cgccgaggtc acggtaggag cccacgtggt ccatcggcgc
     2461 ctcattgagc gccgccgcct cgagcgcgca gcgcgcctcg tggaagctgc gccgcagcgc
     2521 tcccaccggc gacggacggc tagccgcggc gcgcacgtcg cccgcctcgg ccgcgagcgc
     2581 gacccgcgcg gcggctgcca gctcgtgagg ctcggcatcg ccctcgcgcg aatcgatgac
     2641 cgcgcacagc agccggtcgc gcatcgccac gagcgcgcca accccggcgc cgccgaggtg
     2701 gcggtcgagg agcggctcgg cggccgcagg atcgtcgacc gagaagacga gcacggcggc
     2761 ggtctcgcca acgccgaagg gacgcaggcg agtggcaagc tcgctcgagt cgagccgccc
     2821 ggcgagcgcc tcggcgagca gatcgccggc gagccggcgc tcggtgtcgc gcatcacgcg
     2881 ctggcgcatc agctcgagcg ccacgacggt cacggcctgc tgcagtatca ggcgctcgaa
     2941 gtcgcccaga ccgccggcgt cgcgcacagc gacgagccac gcctggggcg agctgcccgc
     3001 gcggatcgtg accggtagcg ccagcgagcg cccggcgaga tcggcgtcgc ccggcacgaa
     3061 gggggcggcc gcggagccgt tcgagcccgt gccgctgctt cgccgctcca cctcctcgcg
     3121 cacgagctcg accgcctgct caggcatgcc gcgccggaac gcgccggacg cgatcgtctc
     3181 gccgcgcgcg gagagcacga gcgccgtacc gccgatcgcc gcggcaagcg cacgcaccag
     3241 ctcgtcgagc ccgcgctcct cgagcacgag gcgctcgagc cgcttgtgga tcgcgatccc
     3301 gcgctggagc acctcgtact gctcgttcac gagctgcgtg aacgccttct cggtgatcgc
     3361 gatgaacggc agccggtagg gcacctcgaa caccgggaag tcgagctcgc gcgcgcgctc
     3421 gagaagcgcc tccgggacgt ccttgtggtc gaaccccgtg ccgaagccga gccccgacag
     3481 gtggtggtcg acaagccgct cgatgaactc ctgctggcgc tccggcgtgt cgagctggat
     3541 gcccgtggtg agcaggagct cgccaccgga gagccagggc gtagggtcgg tcagctcggt
     3601 gatgtgcacc cagcggacgg gggcctccgc accgccctct ccggcgacga gctcgaggcc
     3661 catctcgcca acgaggcgct gcacggtgag cacttgccga cctccgcgtt cccttctact
     3721 tgtggtcgat ccggtcccag gcctcgctga gcaccgggcc gagcacggcc tcgatctcgt
     3781 cgaactgctc gggacccgcg atcagtggcg gggcgagttg gatgaccggg tcgccgcgat
     3841 cgtcggcgcg gcagatcagc ccgcgcttgt acagctcgcc cgacaggtag ccgcgcagca
     3901 gctcctcggc ctcctcatgg ctgaagctct gcttggtctc cttgtccttc acgagctcga
     3961 gcgcccagaa gtagccggca ccgcgcacgt ccccgacgat cggcaggtcg gcgaggcggt
     4021 cgagcatcgc cttgaaatcc ggctcgtact gacgcacgcg gccattgagg tcctcgttct
     4081 cgaagatctc gatgttcttg agcgccaccg cggccgccac cgggtgccca ccgaaggtga
     4141 tgccgtgcgt gaacatcttc gtgtcgtcca tgaacggctc ggcgatcttg tccgaggcga
     4201 tcacggcgcc catcggcgag taggccgagg tgatgccctt ggcggtcgtg atcaggtcgg
     4261 gcacgaagtc gtagcgctgc gcgccgaacc agtagcccag gcggccccac gagcagatga
     4321 cctcgtcgga gatcatcacc acgccgtact tgtcgcagat ctcgcgaacg cgctggaaat
     4381 agccgtcggg cggcgtgaag cagccgcccg agttctgtac cggctcgagg atcacggcgg
     4441 atacggtctc cgggccctcg aactcgatgc gctgctcgat ctcctcagcg ggccagagag
     4501 gatcctggcc ctcgggcgcg cggtagcggt tggtgttcgt cacatgacag ccgcccgcgg
     4561 gcagcggctc atagggcgag cggatgcctg tgatgccggt cgccatcagg cagccgagcg
     4621 tggtgccgtg gtaggcggtc ttgcgcgcga tgaccttgta gcgcccggcc tcgccgcgca
     4681 gcttgtggta ctgccgcgag agcttcagcg ccgactcgac ggcctcggag ccgccggacg
     4741 tgaagaacac gcggttgagg tcgcccggcg cgagctcggc caccttggcg gcgagctcga
     4801 tggcgcgcgg gtgcgcgaat gtccagtttg tatagaaggc aaggtcgcga gtctgctccg
     4861 cggcggcgtc acccagctcg gtgcggccgt ggccggcgtt gacgcagaag agggccgaga
     4921 gcccgtcgag ataacggttg ccgtgctcgt cccagacgta gcatccgtca cccttcacga
     4981 ggatggggat ctcatggtcg gtgtacgacc ccatgcgcgt gaagtgcatc caaaggtgct
     5041 gccgggccag atcctgcagc tccgtgtgct cggcgggggc ggtctgcgga gcgccctcga
     5101 gcctcgtcgc catacgttct cctcctgctc gcctctagac gatgcggcta aagctagctg
     5161 cgccttggag caggaacaag ggagccaaag ttcaactgcc gttaagacac ttatcctaag
     5221 cgtacaaaca gcgcgggctg ttttagggtt cgcggcagtt gcgtctccaa ccaggcgcgc
     5281 ctagttttcc ttgttgtcca ggccggtggc gcgatggccc gttcgctggc cgaggaggag
     5341 gagaagaccc aagaagtgac cgcctcgagc gatccccgga tctccggcgg cgagatgccc
     5401 tcggtccggc tccagaacgt gaccaagcgc ttcggcgacg tcgtggccgt cgacgacatg
     5461 tcggtcgaca tcgcacgtgg cgagttcttc accatgctcg gcccgagcgg gtgcggcaag
     5521 accaccacgc tccgcatggt cgcgggcttc gaagagccca ccgccggcgc gatccttgtc
     5581 gacggccggc acatggaggg cgtgcccgca ttcaggcgcc cgaccaacac ggtcttccag
     5641 agctacgcgc tcttcccgca cctgagcgtg gaggagaacg tggccttcgg cctgcggcgc
     5701 aagggcgtgg acaagaagga ggtcacccgg cgtgtgggcg aggagctcga gctcgtcggc
     5761 ctcgcgggcg agcgtaagcg caagccgcgc cagctctcag gcgggcagca gcagcgcgtc
     5821 gcccttgccc gcgcgctcgt gaaccgcccg gcggtgctcc tcctcgacga gccgctcggc
     5881 gcgctcgacc tgaagctgcg caagggcctc caggtcgagc tcaagcgcat ccagcgcgag
     5941 gtcgggatca cgttcgtcta cgtgacgcac gaccaggagg aggcgctcac gatgtcggac
     6001 cgtatcgcgg tcatgaacag gggcatcgtc gagcaggtcg accagccaga ggcggtctac
     6061 gaccggccgc gcacgacctt cgtcgccggc ttcatcggcg tctcgaacct gatgccgggc
     6121 gcggttcagt ccgcgaacgg ccacgacgcg gaggtgcggc tcgacagcgg cgtcaccgtg
     6181 accgcgccta ccgatgggct ccgcagcggc gagcgcgtgc acgccgtggt gcgcccggag
     6241 aagcttcagc tcgatccggc cgaaactccg tccgtggatg accggccgaa cgtggaaggg
     6301 ctgctggaga gctcgctgta cctcggcacc gccacgcaag tcgtggtgcg cctcccagac
     6361 ggcgtgtcca tgaccgtgct cgtgccgaac accgacgagg aggcgaggcg gcgtctcccc
     6421 ggcgcgggcg cgcaggtgcg gctgtcgtgg gcgagcgagc acatgcatgt ggtccgggag
     6481 gaggggactc cggtccacgc gaacggggcg atggacggcg acggggccgc tctgccagac
     6541 gccataccga cgccggcgga accggcgctc actgaggagg ggaagtgacg gacatcgacc
     6601 gcgcgattga cgagctcatc cgccaggagg tcacgcgcag gcggctcctg cgcaatgccg
     6661 gtgccggcgc gctaggcgtg agcgccgccg cgttcctcgc cgcgtgtggc ggcggcggca
     6721 tcgagggcgg gagcaacaag aaggagtcgc aggtcatccc gaaggggaag atcgcgacca
     6781 cgctcaactt ctcgaactgg ccgctctaca tcgacgtctc cggcaagcgc cacccgacgc
     6841 tcgacgagtt ccagaagaag tacggcacga aggtcaagta caccgaggag atcaacgaca
     6901 acaccgagtt cttcggcaag gtgcgccagc agtacgcggc cggcaactcc ggcggccggg
     6961 acctccaggt cgtcaccgac tggatggccg cgaagatgat ccagctcggc tacgtgcaga
     7021 agctcgacaa gtccgcgatc ccgaacgtcg tgaacaacat gaccgacgtt ctcaagaacg
     7081 cgccgatcga cccgaagcgc gactacacgg ctccatggca gagcggccag accggcctga
     7141 tctaccgcaa ggacaaggtc ggcactccgc agagcgtcaa cgacatcttc gacccgaagc
     7201 tcaagggcaa ggtcacgatg ctcaccgaga tgcgcgacac cgtcggcctc gtgatgcttg
     7261 gcatgggcaa ggacccgcag agcttcacga acatggacga tgcgctcgcg gcgatcgaca
     7321 agatcgacaa ggcgaacaag gacggccaga tccgtcgctt caccggcaac gactacctca
     7381 aggacctgcc gaagggcgat tcgtggctgg tgttcggctg gtcgggcgac gcggtgcagc
     7441 tcgagtcgga caacaagaac atcaagttcc agcagcccga cgaagggttc atgctgtgga
     7501 ccgacaacat gcaggtccct gtcggggcgc ctcacgcgta caccgcagag gtctacatga
     7561 actacgtcta cgaccccgag gtgcaggcga agatcaccgc gtacgtgaac tacgtgccgc
     7621 cggtgaaggg cgtccaggag atcatcaaga aggacgatcc gaagctcgcc gagagcccgc
     7681 tggtgttccc cgacgcacag gtgctcagcc gggcgaagca gttcaagaac ctcacgcccg
     7741 accaggagaa cacgctcaac acggcattcc agaaggtgat cggcgcctag cgccgcctga
     7801 ctcgcgtgtc ggccctgctc cagcggcgaa agggcgttct gccctatctg ttgctcgccc
     7861 caggcctcgc ctggctggtg atcttcttcc tgatcccgct ctactacatg gcgcggatct
     7921 cgctggagac agggtcggtc ttctcggggt actcgttcag ctggcacttc gccacctacg
     7981 gcgacgcgct cagcacctac tggccgcagc tcgagcgctc gctgatttac gcgctggcga
     8041 caaccgggat ctcgctgctg atcgcctatc cgctggcgta cgcgatcgcc acccgcggcg
     8101 gccgctggcg aaacgcgctc ctgctgatgg tcatcctgcc gttcttcgtc acgtacctgg
     8161 tgcggacgct cgcgtgggag accatcctcg acgactcgag cttcgttgtg aacgctctga
     8221 agacgctgca gatcgtgccg agcgacggac gcgtgctggc gaccacgggg tcggtgatcg
     8281 cggggctcac ctacaacttc ctgccgttca tgatcctgcc gctgtacgcg agcctcgagc
     8341 gcatcgacaa gcgcatgctc gaggctgggt acgacctata cggacggcgg cgcgacgtgt
     8401 tcctgagggt cactttcccg ctgtcgctgc cgggagtggt cgcgggcgtg ctgctcacgt
     8461 tcatcccagc ggcgggcgac tacgtgaacg ccacgctgct cgggagcccg cgccagttca
     8521 tgatcggaaa cgtgatccag agccgctacc tggagctcac tgactatccg actgctgcgg
     8581 cgctgtcgtt cgtgctcatg gcgatcatcc tcgtgttggt ggcggcctgg gcgaagatcg
     8641 ccggcaccga gtcgctgatg gggtctgagg ccagatgacg ggcgcgaccg tcgcacagca
     8701 gccggccccg gcggttcccg acgcgcggcg gcgcgacctg ctgaaggcgg ggcgctggct
     8761 tctgaaccag tcgctgaacc tctacgcggc cctggcgatt gtctacctgc tggcacctat
     8821 cgcggtggtc atctggttct ccttcaacga cacgcacagc cgcttcaact tcgtgtggca
     8881 gggcttcacg ctgaggaact ggaaggaccc gttcggcgtg cccggcctgc tcgacgcgct
     8941 gaaggtctca ctcgagatcg ccgcgctgtc gacgatcatc gccacggtgc tcggcacgat
     9001 gatggcgctc gcgctcgtgc gctaccagtt ccgcgcgcgc ggcgccacga acttcttcat
     9061 cttcctgccg ctcgccacgc ccgagatcgt gctcggcgct tcgctcctgt cgctgttcct
     9121 gtcgctcggc gtgaacaccg gcttcgccac catcctgatc gcacacgtga tgttcaacat
     9181 ctcgttcgtg gtcgtgaccg tgcgctcgcg gcttattgga ttcgacagat cgctcgagga
     9241 ggccgcgcag gacctcggcg cgaactcgct ccagaccttc atgcgcgtga cgctgcccct
     9301 gatcgcgccc ggcgtcctcg ccgccgcgct gctcgcgttc gccttgtcgg tcgacgactt
     9361 cgtgatcacg aacttcaact caggcgcgac cgttacgtac ccactcttca tctggggcgc
     9421 ggcgcgcgtg tcgatcccgc cgcaggtcaa tatcgtcgcc acgatgatgt tcctcgtgac
     9481 ggtggccgcc gcgatcctca ctgtgctgca acagcggcgc gccgagaaga tcgcctcggt
     9541 acgtcctgag caggaagccg tcgcttagtg agcctctggg agcgggtcgc cggccttccg
     9601 atcgaggtcg agtcatacgc gctcgaaggg ctcgagcgcg acgtgtcgag cgacttcacg
     9661 cgcctctcca cggtcatcca catgagcggc ggtggggagg aggggctcgg cgaggacgtg
     9721 acgtacgacg gtatggatca catcgctctc caggatgccg gtccggttca tgacctctcc
     9781 ggcgtgcgct ccttcggtga gttctgcgag ctggttggct cgctcgacct cttccctgct
     9841 gagcccgtgc gcgaggtgtc gcgtctgtat cgccgctggg cgttcgagtc ggccgcgctc
     9901 gacctggcgc tgcgccaggc gggccgctcg ctcgccgacg ttgtggagcg ccagcctcgg
     9961 ccggtgaact tcgtagtgtc gctgcgcctt ggcgacccgc cgacgatgga gccgatccgg
    10021 aagcggctcg acctctaccc ggagctgcgc ttcaagctcg atcctgtcca gggctgggac
    10081 gacggcctca tcagcgacct cgtcgcaacc ggcgcggtcg actcggttga cctgaagggc
    10141 ttctacaagg ggacggtggt cgacaacccc ggtgatgcgg gtctctactc gcgggtgatc
    10201 gaggcgttcc cggccgcctg ggtggaggac cccgacgtca acgaagagac gcgcccgatc
    10261 ctcgagccga tccgggaccg catcacctgg gacgcgccga tccactccgt cgccgacatc
    10321 gaagagctcg agtggaagcc gcgcatggtg aacatcaagc cgtcccgctt gggcagcgtg
    10381 caggagctgt tcaccgctta cgactactgc gacgcgaacg gcatcggcgc ctacggcggc
    10441 gggcagttcg agctcggcgt gggccggggg cacatccagt acctcgcgtc gctgttccat
    10501 cccgacacgc cgaacgacac cgcgccgggc ggctacaact caacgaaccc cgagcccggc
    10561 cttccctcga gcccgctcgc ccccgcgata gcgccggcgg gcttccggtg ggaggcgccc
    10621 gtcgcctgag atcggcgcgt acctcaccga gcttgtgcgc cgggtgcgcc aacagctcgg
    10681 ggacgagctc gtgggcgtga tcctcgcggg gtcctacgcc accggcgagg cgctcgacgt
    10741 gagcgacgtg gacgtggcgg tcgtgtgcga gcgggagctc gatcccgtcc gcaagcgcgc
    10801 gctcgtcgcc gccctgcggc acgacgcgtt gcccggcccg gcccggaagc tcgagctcgc
    10861 tgtctacagg cgcgacgtcg tcgagtcgcc aggacagacg gtgccgttcg agctgaacct
    10921 gaacagcggc cgttacgagg agcacgtgtc atatgacccg gcggaggagt cgccgcactg
    10981 gttcgtgctc gatctcgcga tcgcccgcgg tcagggccgc gcgctctacg ggcccaagcc
    11041 ctcggagctg atcggggaag tcgatcccga cgcggtgcgt gcggcactgc tgcaggcgct
    11101 cgactggtgg cagcgcgagc tgccggactc gccgaacacg ctcctgagcg cctgccgtgc
    11161 gtggcgctgg gccgagcagg gcgactggct ctctaaggtt gcggccggcg agtgggcgat
    11221 gaccaggagc gctcaccccg aggcgattgc cgccgcgatc gagcggcgtc cgctgcgcga
    11281 gccggtggaa gcggcgctgg tggcggcggt gagcgacgag gcacgcgagg cactcggcag
    11341 aaggcgttga catcctcgac tgcttcacgc cacggcgcag gggagtcggc ccggcgttca
    11401 gtttcgcgac aggatgtcgc gcacgatcag cgtcgcggcg gccggtgagc ccttgacgaa
    11461 gccgaagaat ggtccgaagt ctcgtgtcgc cgagaagccc gtgacgtaga ggccggcgag
    11521 gttcgtcgcg aaggactcgt cgagcacggg gaagccgtcg gccagcgtga tgcgtccagc
    11581 gagcgtcgcg agatagggga ctcgcgtgag atcggcccgg tagcccgagg cgaagatcac
    11641 gcgatcgatc gccagccgct cggagttcga cagcagcaca cgcagctcgc cgtcggcgcc
    11701 cctggtggtg acctcgacga cctcggtgcc cggccatcgg tggatcccgt gccagtcgag
    11761 ccgcggagtg agccagtgct cgagagtcag ccggccgacc tcccagaact tccgggcgat
    11821 cgcatcctgc tccgcctttg ggaggttccg ccagtagccc gggacgttcg ccgtgttctc
    11881 cacgtaggcg tccacgaact tccagctcac cttctcgaac cgcggcacgt catgccggtg
    11941 caccaagtcg attcgctccg cgccatgctc gcggatcagc gcagcccatt cataggcgct
    12001 ctgccttcca ccgacgatca ggacgcgcgc gcccgcgagc tcgtcgaagc gcacgaggtc
    12061 acaggtgtga gcggcccggc cggcagggac ggaagcggcc cagtcgggaa cgttggcgta
    12121 gtgacggata ccgggtgcac atacaacggc gaacgcctct atgcgttcgc cgccgtcgag
    12181 cgtcgcctcg aacccgccgt tcgactcggc gagctcggcg acgagctctt cgcgcacatc
    12241 gagtcccttc gcctgccgga accactcgca gtagtccagg aacgtgcgga tcgggatcgg
    12301 atccacgtcc tccgtggcga tcttccgatc ctccagatac gcctcgagcg tgtgtacccc
    12361 ggcggcgtcg atgtgccagt cgggcccgga acgcaggaac atccccgcgg gcatgtgctc
    12421 ccgccagaag ctcatcggct gcccgaggat cagtgtctcg atgccgtgct cgcgggcaaa
    12481 ggcggcggtc gagagcccgt agggcccggc gccgatcacg agaagctccg ttgccatcct
    12541 cgctcactcc agcctgcgac tgcccacgcc gggccgccca gacggggtgt gacgttcccg
    12601 gcggctacct cagagtacgt gcgaccacga ggcgctggat ctcgttggtg ccctcgtaga
    12661 tctgcgtgat cttggcgtcg cgcatgaagc gctcgacggg gtactccttc acgaagccgt
    12721 agccgcccag gacctggacc gcctccacgg tcacgtccat cgccgtgtcg gacgcgaaca
    12781 gcttcgccat cgcggagtac ttgcccatgt cgggctcgcg gcggtcgacc ttcgcgcacg
    12841 ccttgtagag cagctcgcgc gctgcggccg tgcgggtctc catgtcggcg agcttgaact
    12901 ggatcgcctg gaaggagttg atcggcttgc cgaactggcg ccgctcgcgc gcgtatgcgg
    12961 cggcgtaatc cgtcgcgccc tgagcgatgc cgaccgcctg cgccgcgacg ccgagccgcg
    13021 agcggtcgag cgtggccatc gccaccttga agccctcgtt cacggtgccg atcaggttct
    13081 ccgccggcac gcggacatcg ctgaacaccg gctggccggt cggcgacccg cggatgccca
    13141 tcttgtgctc gagcttgccg atcgagacgc cgggacggtc ggcctcgacg acgaacgccg
    13201 agatgccgcg cgagtgcccc tcgtccgggt cggtgaccgc gaacaccacg aacaggtccg
    13261 cgatgcctag gttcgtgatc cagttcttct cgccgttgat cacccactcg tcgccgtccc
    13321 gcatggcgcg cgtgcgcatg ccgcccgggt cggagccagc ctcgacctcg gagagcgcga
    13381 acgcgggcga ccactcgccg ctcgcgagct tggggagcca gcgctgcttc tgctcgtcgg
    13441 tgccgaagag cttgatcggg agcgagccga gctcttggat catcaggatc agcgccgttg
    13501 atgcgcacgc cttcgagagc tcctcgaccg cgatgttcag cgtgagcgtg ccggtgccgg
    13561 tgccgccgtg ctcctcgtcg aacggcaggc cgaggatgtc ctgctccgcg agcaacttcc
    13621 gcaggtcgtg cggatactcg gccttctcgt ctatctccgc ggcgcgtggc gccacacgct
    13681 cctgggcgat ctgccggacg aggtcgcgga acgcgatctg ctcgtcgttg agcgcatacg
    13741 tcatgtcggc ggtcgcggcc atgcggaagg atgcatccta gtgaccccgc ccgggccgac
    13801 gtcagcccct gtcctggtcg ctccagacag cttcaagggc agcttcagcg ccgccgaagt
    13861 ggcggtcgcg ctcgcccgcg ggctcgaggc gggcgggctt ccggcgacgg aactgccggt
    13921 cgccgacggc ggcgagggca cgatggacgt cctcgtgggc gcgctcggcg gcgagtaccg
    13981 caccgcgcgg gtgagcgacc cgctcgggcg gcccgtcgcc gcgcgctacg cgctgctcga
    14041 cgacggcagc gcggtggtgg agatggccca ggcgagcggc ctcgggctcg tggacgagag
    14101 tgaccgcgac gcgttcgcgg cgtccacgcg cggcaccggc gagctgatcg cagcggcggc
    14161 cgaagcgggc gccgcgagcg tgctcgtgac ggtgggcggc agcgcgacca ccgacggcgg
    14221 tgccggcgcg ctcgccgcgc tcgacgaggc gggcatcaag ccgaagctcg aggtcctctg
    14281 cgatgtgcgc acgccgttcg agctcgcacc ccgtgtgttc gcgccgcaga agggtgccga
    14341 tcccgcaacg gtgaaacgcc tggagcgccg gctcggccgg atcgcgcgcg aggctccgcg
    14401 cgatccgcgc ggcgtgccga tgaccggcgc ggcgggcggt ctctcgggcg ggttgtgggc
    14461 gtaccgcggc gcgaagcttc tgccgggtgc cgcgtacctg ctcgacgcga tcggcttcga
    14521 cgacgccatg cgcgcatcgc gcttcgtcgt gacgggcgag gggcgtctcg atgagcagac
    14581 cctcgagggc aaggtggtgg gtgaggtcgc cacacgctgc cgccagggcg gggtcgcgtg
    14641 ccatgccgtc gtgggaaacg attcgctcga cgagttcctg gagcgcgtga tcgacctcgc
    14701 gagtgtgcgc gaggcgacga cgctcgccga gatcgaggcg gctggacggt cgctcgcgtc
    14761 gtttagctga ccttcagcga ggcagtgaat cctgagcggc ggcccggcgc gccggacggg
    14821 aagtgtccat gacctctaac gcggcgacaa ggaagctgcg cggctcccgc gctctcacgc
    14881 tgcgcatgcc ggagctccgc cacgcgctgg cggcactgtc gctcgccggc atcgtggtca
    14941 cgggtgcggt gctcgcggcg gggtcgagtc cgcatcgctc gttcctgctc ccggtgagcc
    15001 gcggccgcgc gccggaggcg atcaccggcc cgcttgccga aggcttcgga ctcaccggcg
    15061 agcagttcct gctgttcctc gtcctgatgg gcggcttcta cggagccttc ctcgtgtggg
    15121 gtcggcacct gagcgctcgc tggaccatga gcgcgattgg cgtgctggtc gtcctgttcg
    15181 cggtcgcccc gccgatcctc tcgaaggaca tcttcagcta cgtcgcctac gcgcgcatgg
    15241 gcgtcgacca cggcgtcgat ccgtacctgc acgggccagc gatcgcgccg cacgacccgg
    15301 tgttcgcgtt cgtcgggtgg aggcacgcgc ccaccgccta cgggccactc ttcacgctcg
    15361 ggagttacgc gctggcgccg ctcggagtcg ccggcgcgat gtggatgctg aaggcgatcg
    15421 ccgccgcgtc ggcgctcggc acctgcgcgc tgatatggcg ttgtgctggt cagcgtcgcc
    15481 ttgcgcaggc ccccgctgtg gccctgttcg ggttgaaccc gatggtgctg gtgtacgcgg
    15541 tgggcggcgg gcacaacgac ctgctgctgt tgcttctgct caccggcggc gttgccctgt
    15601 gtctgaatga gcggcccgcg gcgggggcgg ccggcgcggt ggcggccacg ctcaccaagg
    15661 cgaccgcagc cgtcacgctg cccttcttct tgctcggcgc ggccgaccgg cgccgcggct
    15721 tgatcggcgg gctcgccgcg gctgttgcgg gcctggcggt cgcaaccgtg gcattccccg
    15781 accacgcact cggtgtgctg cgcgtgctcc gccacgagtc gctgttcgtg tcggccgaga
    15841 gcgtcccgac cgagctctcg cgcctcttcg gagccgaaac cgtcacgccg gctgcccacc
    15901 tgatcgcggc gttggccctc ctcgccgggc ttgcgtacct cgtggtccga gtttggcgtg
    15961 gaatggactg gatcgccggc gccgggtggg cgctcgcgct cacactggtc accagctctt
    16021 ggcttctcgg ctggtacacg atctggacgc tgccgttcgc ggcgctggcg cgcgaccgcc
    16081 gtctgaagat cgtcgcgctc gcgatctgcg cctactacgt gctggggcgc tggaacatct
    16141 tcataggcgc ggggtgagcg ccgcgggcac gagctgggag ggcagcttgc gccgagtcgt
    16201 caccctcagc acgcgcacgt gtggaccttt cgtggtgagc gcacagagct tcaagtggtc
    16261 ccttcgcggc ctgtgatgca cgagcagcgg cgcgcacacc atgttctcgc gggtgccgag
    16321 acgcaccacg gatttcccga cgtggtgtag ctcgccgtgt cgcgcgagcc actgacggaa
    16381 ggccaccttc tcggtcctca ccgtgcgtgc cccgtgcacg tccctgagag cgttgatcag
    16441 cacggccgcg ccgagcagca gcgcaaacag gatcaggatc gcgtcaagaa cccgcgacga
    16501 gcgcgtagat ctccaccatg cccgcctggg tgaggagccg cttttccggt cgctcgctgc
    16561 cgaagatccc cgtgtggggg tctctcgatt gattccacgc ctgatccgca tagcgctcga
    16621 gctgggtccg gaagctcgtg ccgcccgcga gctgttcgta ggccaacagg tttctgaagt
    16681 agatcgccag aaagtaaggc ggctctgaag gcaggcggct gtcctcgaaa tagtcgagcg
    16741 cgcgggcggc gcgagcggcc gccgtgtcga ggtacgcgcg gtcgccagtt gctcggtaga
    16801 gcgcgatcgc cgcgccgatc atgctgccct ggttgtagct ccagatggtg gtgtcccggc
    16861 tgccgtcggc tcggaagtgg tcctgataga ggccggcttg ctcgtcgagc atgcaccgct
    16921 cggcccaggc gtacatccgc tggccccagc cgagcgcgcg ccggtcgccg gtcgcctcgt
    16981 agatcgcgac cgcgagctgg gcgcccggcc cgttcgtgac tgtgccgcgc tggtagatgc
    17041 gcggcaccgg agtccacttg acaccgccag ggcaagtggg ggcgcggcgg tcccagcccc
    17101 ggatcacgag ccgcaatatg gcccgcgagc gctcgaggag gccgcgctgg cgggtgaccg
    17161 cgtagcggcg catcagctcg agcccgatcc actcgttgtc gtcgtagtag aggtccccgc
    17221 ctgggccgaa aggcgggcgc ggaaggctct cgtacgcgcc cgagcgccag aagcgcctca
    17281 gctgtcgcag gcgcatgcgc gcctcggctc gctcgcgacg ggtcgcgtca ggaagctcgg
    17341 caatcgactg cgccgcggcg accaggcgaa cgaacggcca ggcgtgcgcg cgtgtgtggt
    17401 cgagccggtg ctgcatcgcc gcgtagctcg cgcgcgctcg cgcggccgcg gtggcatccg
    17461 gatctgccac ggcgcggcgc tcgtcgaggg tcgcggcgag acagagcagg gccacgaccg
    17521 cgagcaggcc ggcgagcgcc gcccgccgag cgctcgagtt caggcgcatt cgggcgtaac
    17581 gctagccgct gatgccgagt tggccacagc acgcggctgc tgtccacggg cgtccatagg
    17641 atcgcgattc ggttgcagaa gcgtcgggtc gcacgtactt tggctacgga ggcaggaacg
    17701 cctcgccaaa tgcgaatcgg aatcgcgtcg acactcacct ccgctgcact cgtcctggtg
    17761 ctcgcgggcc cggcctcagc ggacgtgaag agcgtgacga tgaagtacgg gccgatcgac
    17821 atcggcccct accaggcgct ccgttcctcg acctacgtac cgacgcccga gctcgacggg
    17881 ttcgtcacgg agatggatgc tcacgtgatc gacgctgaaa ccgggcagga gatcccgcag
    17941 taccaggtca tgctgcacca cgtggcgttc accgacgagg cgcgccgcga cggctcgtgc
    18001 ccgcgaaaaa acatcggcga gcgcttctac ggcacgagtg aagagctccg ccggatgacg
    18061 ctgccggccg gctacggcta ccggatcggc gcgcgcgaca agtggcggat gatctggatg
    18121 ctcatgaatc acctgaagac cgacggcaag gtgttcatcg agtaccacgt gaccgtcgac
    18181 acctcgccga gcctcacgcc agtgcggccg tactggataa gcgtcgtgcc gtgcgtgaac
    18241 gacccgcagt actccgtccc cggaggcgcg acggccggcg ccacgagctc gcggaaggtc
    18301 acctggtccg tgccggtcag cggccggatc gtcgccatgg gcggccacct ccacggcggc
    18361 tcgcgcagcc tcacgctcac tcagccgcgc tgcggccacc gagtgcttca caacgcgttg
    18421 cccacgtacg cgccgccggg ggacccgctc tacacagtcg tgccactgct gcacgagccg
    18481 gaccccgtga acgtgagctg gtggcagtcg gcgaccggct ggaacgtcca aaagggcgag
    18541 atgctgcggc tgtcggcgaa ctacgacggc caccgcccgc acatgcgcgt gatggggatc
    18601 atgcacgtgt acatcgccga ggacgagggc gtaggggcgt cagcctgcgg cgccaagccg
    18661 gcggacgcgg aggtactggg tggaagcgcc gtggcaggtg ggcgcctcgc gccgccgtcg
    18721 gtcgtcgtgc ccttcgaggc ggccgggcgc gacggagtgg cccggccgat ctcgcgatcg
    18781 cccggtgcgc tgcgtcgcta cgacggcgac gcgaacgtga ccgtggccga cttccgctac
    18841 agcgtggcga acctctcggt gccgcgcggc gcgacggtgc gatggcactt ccgcgacagc
    18901 gcggcccacg acgcaacgct ggtgaacggg cctcgcggtt tcggcacccc gaccgtgcgg
    18961 aagggccacg tcgagtcgca gcgcttcacg gtgccgggcg tctaccgcat ctactgctcc
    19021 atccaccccg tctacatgtc gcaagttgtc cgagtcaggt ccaagcgctg aggtgatggt
    19081 gcgcgtgcgc gcgccccggc tcgacgacca ggcggcgttc ttggcggcgg tcgaacgcag
    19141 ccgtgcgtcc caccggcctt ggagcttccc gcccgacagc ccgcagctct acagggagta
    19201 cgtcacgaag atcgctgccg acgactgcga gggcttcctc atcgagactg gggacggtga
    19261 cctggcgggc ttcgccacga tcagccagat cgtgcgcgcg ccgctgctgt ccgggtatct
    19321 cggctacacc ggcttcgagc cgtacatggg ccagggcatc atgcggcgcg gcctgatcca
    19381 gatcctcgat cacgcattcg gctcgatggg actccaccgg atcgaggcga acgtgcagcc
    19441 cggcaacgag cgctcgatcg cgctcgttca gcggctcggc ttccggcgcg agggcttctc
    19501 accgcgctac ctaatggtgg gcggcgtctg gcgcgaccac gagcgctacg cgatcacggc
    19561 cgaggaatgg cccccgcctg gggtgggccc ctagccggcg aggatccggc gcgtctcggc
    19621 gcagcgccag tgtcgacaag ccgtagccgg accggagctc cggctctccc tagccggcga
    19681 ggatccggcg cgtcacggcg cagcgcagtg tcgcgacaag ccgtagccgg accggagctc
    19741 cggctctccc taaaaccgct cgatctcgct cagcaggtag tcgtacgcgg gcagcgtaag
    19801 gaactcgggg aagtcctccg cgagcgcgac ctgctcgaaa agcgtgcgtg acgcttccgg
    19861 ccggccctgg ttctcgaacc actcgtcgtc cccgatctcg ccgcggatgc gctcgagctc
    19921 ctggtgctcg agccggtgga cgagctccgg gttcaccttg cgaccgtcgc tcagctccgc
    19981 gccgtgccgc acccactgcc acacctgcga gcgcgcgatc tccgcggtcg cggcgtcctc
    20041 catcaggccg tagatgcccg cggcgccgtt cccgcgcagc cacgacgaga tgtactgaat
    20101 gccgacgttc acgtcgttgc gcaggccctc gtctgtgatg tcacccggcg tggcctgaac
    20161 gttcagcagg tcggccgcgg acacctccac ctcgtcgcgc tgccggtcca cctggttctg
    20221 gcgctcgccg agcacgcggt cgaactcctc ggtcgcagcc gggacgctgt cggggtgggc
    20281 cacccaggtc ccgtcgaagc cgtcgcccgc ctcgcgccgc ttgtcctcgc gcagcttggc
    20341 gagtgcgcgc tcgttcgcct cggggtcgcg ccggctcggg atgaacgccg ccatgccgcc
    20401 catggcgtgt gcgccgcgcc ggtggcaggt cttcacgagc agctcggtgt aggcgcgcat
    20461 gaacggaacc gtcatcgtca cgctgttgcg gtccggcagc agaaactccg ggcggtccct
    20521 gaagcacttg atcatcgaga acatgtagtc ccagcggccg gcgttgagcc cggccgagtg
    20581 gtcgcgcagc tcgtagagga tctcgtccat ctcgaacgcg gcaggcacgg tctcgatcag
    20641 cacggtggcc ttgatcgtcc cgcgctcgat cccgagctcg tcctgcgcgt ggttgaagac
    20701 ctcgttccag agccgcgcct cgaggtgcga ctccatcttc ggcaggtaga agtagggacc
    20761 gctgccgcgc tcgagcagcc ggcgggcgtt gtggaagaag aagaagccga agtccaccag
    20821 cgcgcccgcg atcggctcgc cgtccacgct catgtgcttc tccggaaggt gccagccgcg
    20881 cgggcgcacg agtagcgtcg ccacctcgtc attgaggcgg tactccttgc cgtcgtcctg
    20941 cgtgaactcg atcgtgcggt cgatcgcgtc ggtcaggttc acctggccct cgatcatgtt
    21001 ccgccacagc ggcgagttcg agtcctcgaa gtccgccatg aacccgcgcg cgcccgagtt
    21061 cagcgcgttg atcaccatct tgcgttcggt ggggccggtg atctccacgc gccggttctg
    21121 aaggtcgggc ggcggcgggg cgacccgcca gtcgccctcg cggatctcgc gcgtcgagtc
    21181 gaggaagtcg agcgagccgc ccgcaggcag gtcggacatg cgctcggcac ggcggtgcag
    21241 cagttcctgc cgcgtgtcct cgaacttgcg gtggaggccg ctcaggaact cgagcgcgtc
    21301 cggcgtcagc acctcctcca tccgcccctt gaccggtgcg tggatctcga ggcccttcac
    21361 ctcaacgcgt gccattgctg cctccctgta tggacatgtg aatcagctca atcggatgca
    21421 cgacgcggag aggctctccc agctgctcac tcgtcgcggt gatctggatc gcacagcccg
    21481 ggttggcggc ggcgatcaac tcggcgcccg tcgcgcgcag gttctccacc ttgcggcgtc
    21541 caagctccct ggcgggctcc gggttcagca ggttgtagat gcccgcggag ccacagcaga
    21601 actcccaccc ggcgggctcg accagctcga gctccgggat ctgcccaagg agctcccggg
    21661 gctgggcgcg gactgcctgg gcgtgcgcga ggtggcaggc gtcgtggtag gcgaccttcg
    21721 cgggcactgg atgccggcgc gcgcgcggag gctcctccgc cagcagctcg gttacgtcac
    21781 ggaccttgcc ggagaagttg tgcgcccgct cggaccactc cggatcgtcg cgcagcaggt
    21841 gggcgtagtc cttcatcgag gagccgcatc cggcagcgtt tgcgaccacg tagtcgcagt
    21901 gctcgaatgc gttgatcgtc gcgcgcgcga ggtcccgcga gtcgtagccg gtgtgcatct
    21961 ggagcgcgcc gcaacatcgc gggagccgcg gcgcgtgcac ctcatagccc tccgcggcga
    22021 gcacctcggc ggtcgcggcg ttcacgtcgt ggaagaacac acgctgcacg cagccctgaa
    22081 ggaagccgac ggtggcacgc cgctctccgc gggcctgcag gcgctcgggc agccgcttca
    22141 tcgtcttgcg caacggtgtg tccggcgtga gcgatgcgag cgcacgcagg cgcgggaacg
    22201 ggccgccgcg acgcgcgagc cgatgcattc caagcttgcg cgaaagcgcg agcagcggcg
    22261 cggccacccg cagccggcct gggtgcggga acagctcgaa cagcatgctg cgccagagcc
    22321 ggtcccctgg ggcgcggctg taattgcgct ccacctccgc gcgcgtgtcc tcgatcagct
    22381 tgtcgtactt cacgcccgat gggcaggcag tcacgcacgc catgcagccg aggcagcggt
    22441 cccagtgcat gaccatctcc ggcgagatct cgcttccgac cactgcctcg ctcatgagga
    22501 cgatgcgccc gcgcggcgag tccatctcct cgccccagac ctcgtacgtc gggcatgtgg
    22561 ggaggcagaa gccgcagtgc acgcactcgt cgatcagctc ctgcgcgggc ggccggatcg
    22621 tgtcgaagct cgccatcaga tgccgcccgc gaaggttcct gatgcgaaga cgcccgcagg
    22681 atcgaagcgc tgcttcacgc gccgcgccag ctccgacgac tccgcgtccc agacgtcgag
    22741 cgaggcgcgc agctcgcgcg gcgcgtcgag cacgacgcac gggtacggcg cgagctgctc
    22801 gcgaagcttc gtcacgcgct cgggcgcaac ggtgacccac gagatgccca gcgacccgcg
    22861 gccgacgagc gacccgccca ggccgtcggc cgcgcgcatc gtgcgcccaa gctcggtctg
    22921 gagcccggag acccgcacca ctgcctgctc ggctgcgcgc tgcccggccc gctgcgcctc
    22981 ccagatgccg tcgtcatcct cgacgatctc tgtgtcgagt cccgcatccg ctagaaggcg
    23041 tgctgccgcc tccgcctgcg catgcgcggt agcgccgccg aggcgcgcga gcgccgcacc
    23101 ctcgccatgg ccccagcgca cgtccacgca ctccttctcg agcgacgcgt gtgcgagatc
    23161 ggatgccccg cgcgcaaccg cctccgggtc gctcgcgctg ccgaccgcgg tcacggtctc
    23221 gctcggccgt gggtggagcc gcacggccac ctggagtacg aggccgagcg tgccgaacga
    23281 tccggagaag agcttggcca ggtcgtagcc cgcgacgttc ttgatcactt tgcttccgga
    23341 cttcgcgacc gtgctgtccg agagcgccac tttcacgccg agcacgaggt cacgcggtgc
    23401 gccgtagcgg tggcgcagcg gcccagaatc gccgctcgcg atcacgccgc cgatcgtggc
    23461 cgcagcgcct tcgccgaggg gcgggtcgag agcgagcatc tggcctgccg cggcgaacgt
    23521 ctgctgcgcg cggccaaggg gcacgccggc ctcgaggatc gcggtcaggt cgccctcgtt
    23581 gtgctcgacg agcgtgtcga gcg
//
LOCUS       DASVXS010000104         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_523510, whole genome shotgun sequence.
ACCESSION   DASVXS010000104 DASVXS010000000
VERSION     DASVXS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523510"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VF752_05905"
     CDS             <1..858
                     /locus_tag="VF752_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855118.1"
                     /translation="ERGERVAAGIKVQNKHRTVGGLLSGEVARRYGGEGLPGDTIQVR
                     LEGSGGQSFGAWLAPGVSFLLEGDANDFAGKGMSGGVLAIRPREGSTFAAEENVIVGN
                     TVLYGATSGRAFFRGLAGERFAVRNSGVSAVVEGVGDHGCEYMTGGRVVVLGPTGRNF
                     AAGMSGGIAYVLDEAGTFAERCNTELVGLETPSPEDFEEIRALVDEHGRRTDSPVAKR
                     VLGDWDQLGGAWVKVMPHDYARALAEAAVKASHDAGGLHGERTAHDRHGHVQETTVAG
                     LSPGAGERI"
     gene            861..2315
                     /locus_tag="VF752_05910"
     CDS             861..2315
                     /locus_tag="VF752_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX8855119.1"
                     /translation="MGELGGFLRLERKNGPKRPIAERVGDYREYQMTLPVAELQQQGA
                     RCMECGVPFCHSGCPLGNLIPDWNDLVYEDRWRDAIDALHATNNFPEFTGRICPAPCE
                     AACVLDINDDPVTIKQIEVSIIDRAFAEGWVVPHVPEVRTGHTVAVVGSGPAGLAAAA
                     ELNKRGHNVALYERNDRIGGLLRYGVPDFKLDKSVVQRRVDLLAAEGVEMVTSVEVGV
                     DITAEELRRRHEAVVITTGSTVPRDLPVPGRELDGVHFAMEYLEQRNRFVAGDDPPEK
                     PISAAGKHVVIIGGGDTGADCLGNSHREGAASVTQFELLPEPPPERPDDLTPWPRWPL
                     ILRISPAHEEGGERLFSVMTTEFTGADGRVTHLHGHEVGPPPEFKKVEDSEFTIQADL
                     VLLAMGFLHPQHEGLVEQLGVGLDGRGNLAASEDDYATSVPGVFAAGDARRGQSLVVW
                     AISEGRKAALSVDRYLASVDAGGELDRVEALARS"
     gene            2327..>2766
                     /locus_tag="VF752_05915"
     CDS             2327..>2766
                     /locus_tag="VF752_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855120.1"
                     /translation="MAAGPDRLPDATHIGSVLLRAAGPEQLRAFYEDTLGVPSGLMRF
                     ERADEVAPRHSTGLYHTAIRFPTREALASALRRAMHSTARMGGASDHGVSEALYMSDM
                     EGNGVELYWDRPREVWPHEPDGRLSMYTIPLDVPGLLELPEVEP"
BASE COUNT          412 a          944 c         1015 g          395 t
ORIGIN      
        1 gagcgcggcg agcgcgtggc ggccggcatc aaggtgcaga acaagcaccg cacggtcggc
       61 gggctgctgt cgggggaggt cgcacgccgc tacggcggcg agggcctgcc cggcgacacg
      121 atccaggtgc ggctcgaggg ctcgggtggc cagagcttcg gcgcctggct cgcgcccggc
      181 gtgagcttcc tgctcgaggg cgacgccaac gacttcgccg gcaagggcat gtcgggcggc
      241 gtgctcgcga tccgcccgcg cgagggctcg accttcgcgg cggaggagaa cgtgatcgtc
      301 ggcaacacgg tgctctacgg cgccaccagc ggccgcgcgt tcttccgcgg cctcgccggc
      361 gagcgcttcg cggtgcgcaa ctcgggcgtg agcgccgtcg tcgaaggggt gggcgaccac
      421 ggctgcgagt acatgaccgg tggtcgcgtg gtcgtgctcg gcccgacagg gcgcaacttc
      481 gccgctggca tgagcggcgg catcgcctac gtgctcgacg aggccggcac gttcgcggag
      541 cgctgcaaca ccgagctcgt cggcctcgag acgccgagcc ccgaggactt cgaagagatc
      601 cgcgcgcttg tggacgagca cggccgccgc accgacagcc cggtcgcgaa gcgcgtgctc
      661 ggggactggg atcagctcgg gggcgcgtgg gtgaaggtca tgccgcacga ttacgcgcgc
      721 gcgctggccg aggccgccgt gaaggcgtcg cacgatgccg gcgggttgca cggggagcgc
      781 acggcgcacg accgccatgg ccacgtccag gagaccactg tcgccggcct ctcgccgggt
      841 gccggagaac ggatttaggc atgggcgagc tgggcggatt cctcaggctg gagcgaaaga
      901 acgggccgaa gcggccgatc gccgagcgtg tcggcgacta ccgcgagtac cagatgacgc
      961 tgccggtggc cgagctgcag cagcagggtg cgcgctgcat ggagtgcggc gttccgttct
     1021 gccacagcgg ctgcccgctc ggaaacctca tcccggactg gaacgacctc gtgtacgagg
     1081 accgctggcg tgacgcgatc gacgcactgc acgccacgaa caacttcccc gagttcaccg
     1141 gccgcatctg cccggcgccc tgcgaggcgg cctgcgtgct cgacatcaac gacgacccgg
     1201 tcacgatcaa gcagatcgaa gtgtcgatca tcgaccgcgc gttcgccgag ggctgggttg
     1261 tgccacacgt tcccgaggtg cggaccggac acacggttgc cgtcgtcggg tcgggcccgg
     1321 cgggtctcgc cgcggcggcc gagctgaaca agcgcggcca caacgtcgcg ctgtatgagc
     1381 gcaacgaccg catcggcggg ctcctgcgct acggcgtgcc ggacttcaag ctcgacaagt
     1441 ccgtggtgca gcggcgcgtc gatctgctgg ccgctgaggg cgtcgagatg gtcaccagcg
     1501 tcgaggtcgg cgtcgacatc accgcggagg agctgcgccg gcgccacgag gctgtcgtta
     1561 tcacgaccgg ctcgacggta ccgcgggatc tgcccgtgcc gggccgcgag ctcgacggcg
     1621 tgcacttcgc gatggagtat ctggagcagc gcaaccgctt cgtcgcgggc gacgatcctc
     1681 ccgagaagcc gatcagcgcc gccggcaagc acgtggtgat catcggcggc ggtgataccg
     1741 gcgccgactg cctcgggaac tcgcaccggg agggcgccgc cagcgtcacg cagttcgagc
     1801 tgctgcccga gcctccgccc gagcgccccg acgacctcac accctggcca cgctggccgc
     1861 tgatcttgcg catctctccc gcgcacgagg agggtggcga gcgtctcttc agcgtgatga
     1921 ccaccgagtt cactggagcg gacggccgcg tcacgcacct tcacggccac gaggtcggcc
     1981 ccccgccgga gttcaagaag gtcgaggaca gcgagttcac gatccaagcg gacctcgtgc
     2041 tgctcgcgat gggcttcctg catccgcagc acgagggcct cgtggagcag ctcggggtgg
     2101 gcctggatgg ccgtgggaac ctcgcggcga gcgaggacga ctacgcgacc tcggttcccg
     2161 gcgtgtttgc ggcgggtgac gcgcggcgcg gccagtcact cgtggtgtgg gcgatctcag
     2221 agggccgcaa ggcagccctt tcggtcgacc gttacctcgc atcagtcgac gcgggcggcg
     2281 agctcgaccg tgtcgaagcg ctcgcgcggt cgtagtatcg gcggcgatgg ccgccggccc
     2341 ggatcgcctg cctgatgcaa cgcacatcgg ctccgtgctg ttgcgcgccg ctggtccgga
     2401 gcaactccgc gccttctacg aggacacgct tggcgtgccg tccgggctga tgcgcttcga
     2461 gcgtgccgac gaggtggcgc cgcgccactc gaccggtctg taccacaccg cgattcgctt
     2521 tcccacgcgc gaggcgctcg catcggcgct ccgccgggcg atgcactcga ccgctcgcat
     2581 gggcggggct tccgaccacg gcgtgagtga ggcgctctac atgagcgaca tggaggggaa
     2641 cggcgtcgag ctgtactggg accgcccgcg cgaggtgtgg ccacacgagc ccgacggccg
     2701 gctcagcatg tacacgatcc cgctcgacgt gccgggcctg ctcgagctgc ccgaggtcga
     2761 gccgga
//
LOCUS       DASVXS010000105        13738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_170604, whole genome shotgun sequence.
ACCESSION   DASVXS010000105 DASVXS010000000
VERSION     DASVXS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170604"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF752_05920"
     CDS             <1..502
                     /locus_tag="VF752_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX8855121.1"
                     /translation="EELASWTGRVREFARERDNARLRTLRVRIEDKGPIVAFHWRGAP
                     DEEAAHARVEQLAGEAEAAGLATHWGRKVLEVRPPVPISKGRAVFELVRGSRAETAMF
                     GGDDATDLDGWDALDELVEDGTLKAAVRVGVRSDEGPEAIVARADLLVDGVPGYQEVL
                     TVLAEA"
     gene            509..1045
                     /locus_tag="VF752_05925"
     CDS             509..1045
                     /locus_tag="VF752_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855122.1"
                     /translation="MRFTEFLKTSVLLFAGSATALVIVAVAGARAKDDTTMLYVGVGW
                     WTIAVAGGLWLGRGSTVTHGIAQLLSTARSTPAVPEVEPGRILFNRMWVLAAFTLVAG
                     GLAFLIPQVPAVASGFALGAALAWRKQARAVEAIEERDGVRFYVEPTSPFKPTRLIRA
                     PWFRKFDALRDEERASVG"
     gene            complement(1042..2796)
                     /gene="polX"
                     /locus_tag="VF752_05930"
     CDS             complement(1042..2796)
                     /gene="polX"
                     /locus_tag="VF752_05930"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX8855123.1"
                     /translation="MPKEMRNAEIAAVFDELGDLYELDGAIVHRVVAYRTAARSVRNA
                     PMSVAELAREGRATELPGVGKTLADKIDALLETGTVPALEKLKAKFPPGLIEMTHIPG
                     LGPKRARKLYEDLGVKNLDELREAAEQERIRALPGFGAKAEENILTALAAGADFRPKP
                     RTLLSRALDTAEGLVAPLRDHPAADRVEIAGSARRWTETCKDLDLIATSHDPEALAEA
                     FGSLSLIGEVHSSGPAGVKAVTNNGISVDLRIVSPANFGNLLQHFTGSKAHNEQLRAD
                     AVRRGIHVSEYGVLDDSTGVTHSCASEEEVYGLLGMAYIEPELREGRGELQAARDGTL
                     PELIRVEDIRGELHCHTTLSDGRNTVEQMAQAAIERGYEYLAITDHSATHGFGNDVQA
                     DALRARIEEVRALDEQLDGLKLLAGSEVNVLPDGSLDYDDDLLAELDWVVASLHSSFR
                     MSEREQTERMIRAMEHPLVDVIGHPTGRLIERREAYALDIDAVIEAALRTGTFLEINA
                     NPDRRDLNELNARRAADAGAMLTIDSDAHGVETLANIRFGVATARRGWITAAQVANTR
                     PWEELDSMRKRGRARARH"
     gene            2819..3535
                     /locus_tag="VF752_05935"
     CDS             2819..3535
                     /locus_tag="VF752_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX8855124.1"
                     /translation="MSGVWLVLPTYNEAENIEPFLRAVVPRLAEAAPAHRVLIVDDNS
                     PDGTGEIADRLAAELDPVEVMHRAGKEGLGRAYLAGFARALDAGADRVMEMDSDFSHD
                     PADVPRLLAACERADIALGSRYVRGGGVVNWPLHRRLLSKGGSWYARHVLGVGVRDLT
                     GGFKCIHRRVLEQLDMRSIQADGYGFQIEVTYRAIQAGFKVEEVPITFKERERGASKM
                     TPWIALEAVWKVPALRFRRD"
     gene            3856..4185
                     /gene="trxA"
                     /locus_tag="VF752_05940"
     CDS             3856..4185
                     /gene="trxA"
                     /locus_tag="VF752_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8855125.1"
                     /translation="MAGNIQEVTDNNFSAEVLESDKPVLVDFWAPWCGPCRVIAPSLE
                     EIAEERDDLRIVKLNVDENQQTAAQFEVMSIPTLIVFKHGQIAHKIIGALPKKRLEQE
                     LEPALAA"
     gene            complement(4212..4610)
                     /locus_tag="VF752_05945"
     CDS             complement(4212..4610)
                     /locus_tag="VF752_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855126.1"
                     /translation="MSSENVELIKAGAPLLRRLLDGEDVPNEEFTILHPDVEITALTG
                     SYRGVDGFKEWVRELRATFGNLPWLAQDFVDAGDLGVLVISTARVEGTHRGTEYEHEL
                     PNLYTVRDGRIVRVRSFASRTEALAAVGQR"
     gene            complement(4615..7713)
                     /gene="ileS"
                     /locus_tag="VF752_05950"
     CDS             complement(4615..7713)
                     /gene="ileS"
                     /locus_tag="VF752_05950"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX8855127.1"
                     /translation="MPHYERVDPQQSFPALEEQVLQRWRERDVFHESVRRREGSKPYI
                     FYEGPPTANGPPGSHHVLARVFKDVFPRYRTMRGHYVPRKGGWDCHGLPVEIAVEKEL
                     GFAKKEDIERYGVAEFNAKCRESVLAYIDEWEALSERIGFWIDTEDAYYTLDNEYIES
                     VWWSLKQVWEQGLLYEGNKVVPYCPRDETALSSHEVAQGYRDVKDPSVFVRFPVKGES
                     GLSLLGWTTTPWTLLSNAALAVHPDVTYVRARVDGERLIVAEPLVERVLGEGAEIEAR
                     MKGSELEGIAYEPPFPYVTDFGPRSHTVLLADFVTTEDGTGIVHTALAFGEDDFRLGE
                     KYGMTVQNPVRSDGTFDERIVPFAGRQVRDANPDIVDALRAEGRLFKEELYEHAYPFC
                     WRCGTALLYYAKASWYVRTTARRGQLLAANEAIDWHPPHIKHGRMGDWLENNVDWALS
                     RERYWGTPLPIWRCAHKHEHCIGSVAELRELAGDAPDDLHKPFIDEITFPCPECGGEM
                     RRVPEVIDVWWDSGSMPFAQWHAPFENDELFKERFPADYICEGIDQTRGWFYSLLAIS
                     TLLFGRSSYETVLCLGLILDPEGQKMSKSRGNVVEPWDVLNTYGADAFRWYYFTSKQP
                     WDGYRFSLETIGESVRQFLLTLWNTYGFLTLYANVNGISRGEPEGGEETELDRWILSR
                     LQATNQIAIERLDDYDTTFAGRAIAEFVDELSNWYVRLSRRRFWDGDPAAFRTLHRCL
                     TTVSKLLAPLTPFVADAMYENLDGSEPSVHLCDYPEPDPAYRDEALEHDMAVVRDAVE
                     LGRSARSQAKVKLRQPLREAVVVAAGREREAISRFEQLVRDELNVKLVRYASEADELG
                     SWELKPNYRALGPRFGKRMPQLADAVAALDAESAADVLRGGGTVGVSIDGEEFPLAAD
                     DLQMALQPLEGYQVERAGTHAVALNLELDDELLREGLAREVVRAIQAARKNAGLNVED
                     RIALTLAGDEELLAAVRAHEDYVTGETLTTSLQLDASGNGAGERIEIEGRSLTIAVER
                     AS"
     gene            7776..9350
                     /locus_tag="VF752_05955"
     CDS             7776..9350
                     /locus_tag="VF752_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX8855128.1"
                     /translation="MPGSGLAHSTVSPVSHDLPRALSPGSLVKSSRARYLFAVIALAG
                     LYWGAAELGYAIEFSGPVAAIVWLPVGVGITFLYLGGMSLWPGVVIGDLLANDYNTLP
                     LGSALGQTVGNTLEVVVATLLMHRLVRRGSPLNSISGLAGMLIAIAVGTAVSATVGAL
                     SLQLGDVIPLSAAASTWRTWWLGDASGALIIVPLAIAWHRPPPREWWTGRRLELTLML
                     IAVAGLSELGLRTEQPLSYLVFPALIWAALRFGQRGATLAIAVATGFTVWETTHYVGP
                     FVYDSITRSVLVTQLFIAVSAISTLCLAVVVSEREAFAEGLRASRARIVEAADTERRR
                     LEHNLHDGAQQRLTALTVRLGLATERVLEHPEIARYVLADAEGELAIAIDELRDLAHG
                     IHPTLLTQTGLANAIRDLAERSAVPVRLIQLPPTRVDDAAEATAYYVVAESLANAQKY
                     SRASRVDIRAALTPGLLHILVADDGIGGADESAGTGLQGLRDRVEAIGGSFGVESSAS
                     AGTRIAAVIPATRLLS"
     gene            complement(9586..9969)
                     /locus_tag="VF752_05960"
     CDS             complement(9586..9969)
                     /locus_tag="VF752_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855129.1"
                     /translation="MGADGLVGVMVVDDQEPFRRVVGELIDAIPEFELVGEATSGAEA
                     LSSLEELAPDFVIVDKRMPGITGIETAGLIMARRPGTVVVLVSLEAPDQTVLGSTGAA
                     AFVRKQEISTRLLRRIWHEASSGSH"
     gene            complement(9993..10427)
                     /locus_tag="VF752_05965"
     CDS             complement(9993..10427)
                     /locus_tag="VF752_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8855130.1"
                     /translation="MSRLEIGDALAHRALEALVRAESSVRRTLAAELEREGISAAGFS
                     ALVVLTTAGGELELRTLRRRLGWSKANATEVVTTLERRGLVRRRRMIDDRRAALVRIL
                     PDGLELVSRVFPHHTERVSRVFGALDETEKRSLAEICRKLAA"
     gene            10517..11722
                     /locus_tag="VF752_05970"
     CDS             10517..11722
                     /locus_tag="VF752_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855131.1"
                     /translation="MIIRKRVTNSTSPRRVLAAAAAVTAVAASSLAGAGGAQAARGME
                     VAVQDDAVFVNNSYYGRDNALDKAQALGATRIRVNAIWSRLAKNPNSSRRPSKREYHW
                     AAIDDLIDSAAARGIRVQLSLTGPVPRWAAGDHRVGVYKPKAHEFALFANDAARHFKG
                     RVDRYSIWNEPNWIGWLKPIASGPRMYRALFVSGYRAIKSADPGAKVLIGETAPRKRG
                     RLSTGPLAFLRGVTCTDANWRRHGCSSLKADGYAHHPYAYEVSPTKVTGGRDDVTMAT
                     LGRLTHALAKLRANRALRTPGGASLYLTEYGYFTHGRHSLGTRRTSAYLKAGFRLGLR
                     NRAVKQMLQYMLVSPPGGANWDTALLATNGNARSAYFSLKAWTNAAAAHNMIARPSGP
                     IYLPPRPAS"
     gene            complement(11719..12612)
                     /locus_tag="VF752_05975"
     CDS             complement(11719..12612)
                     /locus_tag="VF752_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8855132.1"
                     /translation="MSGIRTNAAAELLGVSPNTLRSWERRFGYPKPRRTSGGHRQYEL
                     AELEALRRALLETHNISSAIEVARQRGEGPTSPARLVESFDRFDESVADRTMEESLAV
                     RSVERTIEDLLLPALEIARDREGREAEYELACRWATGWLHAMRRVAPPATRQEGVLLF
                     DSSPMLDVEAIHVQALELALRRAGFRVLLLSMGLAQERVSRALRALDPTAIVVCGAGA
                     TLDVVGRLVYSVRHADSHARIYEYRDAMPVSGTHSIPSLGSRLGEATANMKALVEGRR
                     LDAPAAGQQAVEPVARLSARA"
     gene            complement(12790..13161)
                     /locus_tag="VF752_05980"
     CDS             complement(12790..13161)
                     /locus_tag="VF752_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8855133.1"
                     /translation="MGVLLAASREALPAEGMSFLVVTSDLRISAVSEAAERLFGSPEP
                     DLIGTNLLRVMTSPESDDELAVRVARAANGARGVALVAIRLIRGDRPVFGRLEARVTP
                     CGPPRGALVALEPMRLVGPSL"
     gene            13552..>13738
                     /locus_tag="VF752_05985"
     CDS             13552..>13738
                     /locus_tag="VF752_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855134.1"
                     /translation="MRGLVLVCGLAAAALASGCGGDHSLPAACREGSGAVTRALSAAP
                     GRVQLADGVPLSRCVRRA"
BASE COUNT         2036 a         4738 c         4778 g         2186 t
ORIGIN      
        1 cgaggagctc gcttcctgga cgggccgagt gcgggaattc gcgcgtgagc gcgacaacgc
       61 tcgtctgcgc acgctgcgcg tgaggatcga ggacaagggg ccgatcgtcg ccttccactg
      121 gcgcggcgcc ccggacgagg aggccgccca cgcccgcgtg gagcagctcg cgggcgaggc
      181 cgaagcggcg gggttagcca cgcactgggg acgcaaggtg ctggaggtgc gccctccggt
      241 cccgatctcg aaggggcgtg ccgtgtttga gctcgtgcgc ggctcgcgcg cggagaccgc
      301 gatgttcggc ggcgacgacg ccaccgacct cgacggctgg gacgcgctcg acgagcttgt
      361 cgaggacggc acgctcaagg ccgccgtgcg cgtaggtgtg cgctcggacg agggccccga
      421 ggcgatcgtt gcgcgcgccg atctcttggt cgacggcgtg cccgggtatc aggaagtact
      481 caccgtgctt gcggaggcct gagccgccat gcgcttcacc gagttcctga agacgtcggt
      541 gctgctgttc gccggatccg ccacggcgct cgtgatcgtg gcggtggcgg gcgcccgggc
      601 gaaggacgac accacgatgc tctacgttgg cgtcggctgg tggacgatcg cggtcgcggg
      661 aggcctgtgg ctcgggcgcg gcagcacggt cacgcacggc atcgcgcagc tcctctcgac
      721 cgcccgcagc accccggccg tgcctgaggt cgagcctggc cgcatcctct tcaaccgcat
      781 gtgggtgctc gccgcgttca cactggtcgc gggcggcctc gcgttcctga tcccgcaggt
      841 ccccgcggtg gcgtcaggct ttgcgctcgg agcggcgctg gcgtggcgaa agcaggcccg
      901 cgccgtcgag gcgatcgagg agcgggacgg cgtgcgcttc tacgttgagc cgacctcacc
      961 cttcaagccc acgaggctga tccgcgcgcc gtggttcagg aagttcgacg cgctccgcga
     1021 cgaggagcgc gcctcggtcg gctagtggcg cgcgcgggcg cggccgcgct tgcgcatcga
     1081 gtcgagctcc tcccagggcc gcgtgttcgc cacctgcgcc gcggtgatcc agccgcgcct
     1141 cgcagtagcg acgccgaagc ggatgttcgc gagggtctcg actccgtggg cgtctgagtc
     1201 gatcgtgagc atcgcgcccg cgtccgcggc gcgtcgggcg ttcagctcgt tcaggtcgcg
     1261 acggtccggg ttggcgttga tctcgaggaa ggttccggtg cgcagcgcgg cttcgatcac
     1321 ggcgtcgatg tcgagcgcgt aggcctcgcg ccgctcgatc aggcgaccag tggggtgccc
     1381 gatcacgtca acaagcgggt gctccatcgc ccggatcatc cgctctgtct gctccctctc
     1441 gctcatgcgg aacgaagagt ggaggctggc gaccacccag tcgagctcgg cgagcagatc
     1501 gtcgtcgtag tcgagcgagc cgtcgggcag cacgttcacc tcagatccgg ccaggagctt
     1561 gaggccgtcg agctgctcgt cgagcgcgcg gacctcctcg atccgcgcgc gcaacgcgtc
     1621 ggcctgcacg tcgttcccga agccgtgcgt ggccgagtgg tcggtgatcg cgaggtactc
     1681 gtagccccgc tcgatggccg cctgcgccat ctgctcgacg gtgttgcgac cgtcgctgag
     1741 cgtcgtgtgg cagtgcagct cgccgcggat gtcctccacg cggatcagct cggggagggt
     1801 gccgtcgcgc gccgcctgca gctcgcccct gccctcgcgc agctccggct cgatgtaggc
     1861 catgccgagc agcccgtaaa cctcttcctc gctcgcgcac gaatgcgtca cgccggtcga
     1921 gtcgtcgagc acgccgtatt ccgacacgtg gattccgcgg cgtaccgcgt ctgcgcgcaa
     1981 ctgctcgttg tgcgccttcg agcccgtgaa gtgctggagc agattgccga agttcgcggg
     2041 cgacacgatc cgcaggtcca cgctgatgcc gttgttcgtg accgccttca ctccggcggg
     2101 cccgctcgag tgcacctcgc cgatgagcga cagcgagccg aacgcctctg cgagcgcctc
     2161 gggatcgtgc gaggtggcga tcagatcgag gtccttgcac gtctcggtcc agcggcgggc
     2221 gctgccggcg atctcaacgc gatctgcagc gggatggtcg cggagggggg cgacgaggcc
     2281 ctccgcggtg tcgagcgcgc gcgacagcag cgtccgtggc ttggggcgga aatcggcgcc
     2341 ggctgcaaga gcggtgagga tgttctcctc ggccttcgcg ccaaagcccg gcagcgctcg
     2401 tatgcgctcc tgctcggcgg cctcccgaag ctcatccagg ttcttgacgc cgagatcctc
     2461 gtagagcttg cgagcgcgct tggggccgag gccagggatg tgcgtcatct cgatcaggcc
     2521 cggcgggaac ttggccttca gcttctcgag cgccgggacc gtgccggtct cgagcagcgc
     2581 gtcgatcttg tcggcgagcg tctttccgac gccggggagc tcggtcgccc gtccctcgcg
     2641 tgccagctcc gcgaccgaca tcggagcgtt gcggacggac cgtgccgcgg tgcggtatgc
     2701 gaccacgcga tgcacgatcg cgccgtccag ctcgtagagg tccccaagct cgtcgaagac
     2761 ggccgcgatc tcggcatttc gcatttcctt aggcactggg gcttactcta cgcggcccat
     2821 gtccggcgtc tggctggtgc taccgacata caacgaggcc gagaacatcg agccgtttct
     2881 gcgcgcggtg gtgccgcgtc tggccgaggc ggcgcccgcg caccgggtgc tgatcgtgga
     2941 cgacaactcg cctgacggca ccggcgagat cgccgaccgg ctggccgccg agctcgatcc
     3001 cgtggaagtg atgcacaggg ccggcaagga aggcctgggc cgggcctatc tcgccggctt
     3061 cgcgcgggcc ctcgacgccg gcgcggaccg cgtgatggag atggactccg acttctcgca
     3121 cgatccggcg gacgtgccgc gcctgctcgc ggcgtgcgag cgcgccgaca tcgcgctcgg
     3181 ttcgcgctat gtgcggggtg gcggcgtggt caactggccg ctccatcgcc ggctgctctc
     3241 caagggaggc tcgtggtacg cgcgccatgt gctcggcgtc ggggtccgtg atctcaccgg
     3301 aggcttcaag tgcatccata ggcgagtgct cgagcagctc gacatgcgct cgatacaagc
     3361 cgacggctac ggcttccaga tcgaggtcac ctaccgggcg atacaggccg gattcaaggt
     3421 ggaggaagtg ccgatcacgt tcaaagagcg cgagcgaggc gcctccaaga tgacgccgtg
     3481 gatcgccctc gaggcggtct ggaaggtgcc ggcgctgcgc ttcaggcgag actgattcga
     3541 gaacgcgacc gtctcctcaa ccggaggctg gacgcgagat cgcccagcgc cgaggttgca
     3601 ttcgctcgtg acggcgagac gctcgcgcaa ctcgaacctg aacgcagtcc gcctcgacgt
     3661 tacgcggacc gccgggtgtg cgcgaccgcg attcccgatc agccgatcgg cctggccgaa
     3721 ctgcgggcgt ctcggacccg tatagtcgcc gggcggttgt gcagccttcc gaggttgctc
     3781 cggtggttcc tatactttgt aaagcattgg gtatgaacaa ctggcggaga cgctgaaacc
     3841 agcggaggaa tgagagtggc tggaaacatc caggaagtaa ccgacaacaa cttcagcgcc
     3901 gaggtgctcg agtcggacaa gccggtgctc gtcgacttct gggcgccgtg gtgcggaccg
     3961 tgccgcgtga ttgcgccgag cctcgaggag atcgccgagg agcgcgacga cctgcggatc
     4021 gtaaagctga acgtcgacga gaaccagcag acggcggcgc agttcgaggt catgtcgatc
     4081 ccgacactga tcgtcttcaa gcacggccag atcgcgcaca agatcatcgg cgccctgccg
     4141 aagaagcgcc tcgagcagga gctcgagccc gccctcgcgg cgtagttttc gccgccgttc
     4201 gtgacggact gttagcgctg gccgactgcg gccagtgctt ctgttcgtga ggcgaacgag
     4261 cgcacgcgca cgatccggcc gtcgcggacc gtgtagaggt ttggcagctc gtgctcgtac
     4321 tcggtgccgc ggtgcgtgcc ttcgacccgc gcggtggaga tcacgaggac gccgaggtcg
     4381 cccgcgtcga cgaagtcctg cgcgagccac ggcaggttgc cgaacgtcgc gcgcagctcc
     4441 cggacccact ccttgaagcc atcgacgccg cgataactcc cggtgagggc ggtgatctcc
     4501 acgtccgggt ggaggatcgt gaactcctcg ttcggcacgt cctctccgtc gagcaggcgc
     4561 cgcagcagcg gggcacctgc cttgatcagc tcaacgttct cgctcgacat cgcgtcaaga
     4621 ggcgcgttcg acggcgatcg tgagcgacct cccctcgatc tcgatgcgct cgccggcgcc
     4681 gttccctgac gcgtcgagct gaaggctcgt cgtcagggtc tcgcctgtca cgtagtcctc
     4741 gtgcgcgcga acggccgcga gcagctcctc gtcgccggcc agtgtgagcg cgatccggtc
     4801 ctcaacgttg agaccggcgt tcttgcgggc tgcctggatc gcccgcacga cctcgcgcgc
     4861 aaggccctcg cgcagcagct cgtcgtcgag ctcgaggttc agcgccacgg cgtgcgtgcc
     4921 ggcgcgctcg acctggtagc cctcgagcgg ctgcagcgcc atctgcaggt cgtcggctgc
     4981 cagggggaac tcctcgccgt cgatcgagac gccgaccgtg ccgccaccac gcagcacgtc
     5041 ggccgcgctc tcggcatcga gcgccgccac ggcgtcggcg agctgcggca tccgcttgcc
     5101 gaagcggggc ccgagtgcgc ggtagttggg cttcagctcc cacgagccga gctcgtcggc
     5161 ctcgctggca tagcgcacca atttcacgtt cagctcatcg cgcacgagct gctcgaagcg
     5221 gctgatcgcc tcgcgctcac gcccggcggc caccacgacc gcctcgcgga gcggctggcg
     5281 gagcttcacc ttcgcctggc tgcgcgcgct ccgccccagc tcgaccgcgt cgcgcacgac
     5341 cgccatgtcg tgctcgagcg cttcgtcgcg gtacgccgga tcgggctcgg ggtagtcgca
     5401 cagatggacc gacggctcgc tgccgtccag gttctcgtac atcgcgtcgg ccacgaacgg
     5461 cgtgagcggc gcgagcagct tcgacacggt cgtgaggcac cggtgaagcg tgcggaacgc
     5521 cgcggggtcg ccgtcccaga aacgccggcg cgaaaggcgt acgtaccagt tggagagctc
     5581 gtccacgaac tcggcgatcg cccggccggc gaaggtggtg tcgtagtcgt cgaggcgctc
     5641 gatggcgatc tggttcgtcg cctgaagccg cgacaggatc cagcggtcga gctccgtctc
     5701 ctcgccaccc tcgggctcgc cgcggctgat gccattcacg ttcgcgtaga gcgtcaggaa
     5761 cccgtacgtg ttccagagcg tgagcaggaa ctggcgcacg ctctcgccga tcgtctcgag
     5821 cgagaagcgg tagccgtccc acggctgctt ggaggtgaag tagtaccagc ggaacgcgtc
     5881 cgcgccgtag gtgttgagca cgtcccacgg ctccaccacg ttgccacggc tcttcgacat
     5941 cttctggccc tcggggtcga ggatcaggcc gaggcacagc acggtctcgt aggaggagcg
     6001 gccgaacagc agcgtggaga tcgcgagcag cgagtagaac cagccgcgcg tctggtcgat
     6061 cccctcgcag atgtagtcgg cggggaagcg ctccttgaac agctcgtcgt tctcgaatgg
     6121 ggcgtgccac tgggcgaacg gcatcgagcc ggagtcccac cagacgtcga tcacctcggg
     6181 cacgcgacgc atctcgccgc cgcactcggg acaggggaag gtgatctcgt cgatgaacgg
     6241 cttgtgcagg tcgtcgggcg cgtcgcccgc gagctcgcgc agctcggcga cagagccgat
     6301 gcagtgctcg tgtttgtggg cgcagcgcca gatgggcagc ggcgtacccc agtagcgctc
     6361 gcgcgagagc gcccagtcca cgttgttctc gagccagtcg cccatgcgcc cgtgcttgat
     6421 gtgcggcgga tgccagtcga tcgcctcgtt ggccgcgagc agctggccgc gccgcgcggt
     6481 ggtgcgcacg taccagctcg ccttcgcgta gtacaggagc gcggtgccgc agcgccagca
     6541 gaagggatag gcgtgctcgt acagctcctc cttgaagagc cgcccctcgg cgcgcagcgc
     6601 gtcgacgatg tccgggttgg cgtcgcgcac ctggcgaccg gcgaacggca cgatgcgctc
     6661 gtcgaaggtg ccgtcgctgc gcaccgggtt ctgcacggtc atcccgtact tctcgccgag
     6721 gcggaagtca tcctcgccga aggcgagcgc cgtgtggacg atccccgtcc cgtcctcggt
     6781 cgtcacgaag tcggcgagca gcacggtgtg cgagcgcggc ccgaagtcgg tcacgtacgg
     6841 gaacggcggc tcgtacgcga tgccctcgag ctccgacccc ttcatgcgcg cctcgatctc
     6901 cgcgccctcg ccgagcaccc gctccaccag cggctccgcc acgatcagcc gctcgccatc
     6961 cacgcgagca cgcacgtagg tcacgtcggg atgcactgcg agcgctgcgt tcgacagcag
     7021 cgtccacggc gtcgttgtcc agccgagcag cgacaggccg ctctcgccct tcaccgggaa
     7081 gcgcacgaac accgaggggt ccttcacgtc ccggtagccc tgcgccacct cgtggctcga
     7141 aagcgcggtc tcgtcgcgcg ggcagtaggg cacgaccttg ttgccctcgt agagcagccc
     7201 ctgctcccac acctgcttga gcgaccacca gaccgactcg atgtactcgt tgtcgagcgt
     7261 gtagtaggcg tcctcggtgt cgatccagaa cccgatccgc tcggagagtg cctcccattc
     7321 gtcgatgtag gcgagcacgg actcacggca tttggcgttg aactcggcca ccccgtagcg
     7381 ctcgatgtct tccttcttgg cgaagccgag ctccttctcg acggcgatct caacgggcag
     7441 gccgtggcag tcccagccgc ccttgcgcgg cacgtagtgg ccacgcatcg tgcggtagcg
     7501 ggggaacaca tccttgaaga cgcgggcgag cacgtggtgc gagccgggcg gcccgttcgc
     7561 ggtcggcgga ccctcgtaga agatgtacgg cttcgagccc tcccgccggc gcaccgattc
     7621 atggaacacg tcgcgctcgc gccagcgctg gagcacctgc tcctcgaggg ctgggaacga
     7681 ctgctgcggg tctactcgct cgtaatgggg cacgagcagc ggattctatg agcgcggact
     7741 aggcacccag gcccgagtct gggatgctgc ccccgatgcc cggatccggg ctcgcccact
     7801 ccaccgtctc cccggtatcg cacgatctcc cgcgcgcgct ttccccgggg tcgctggtca
     7861 agagttctcg cgcgcgctac ctctttgcag taattgcgct cgcagggctg tattggggtg
     7921 cggcggagct cggctacgcg atcgagttct ccgggccggt ggccgcgatc gtgtggctgc
     7981 ctgtcggcgt ggggatcacg ttcctctacc tcggcggtat gagcctctgg cccggcgtcg
     8041 tgatcgggga cctgctcgcc aacgactaca acacgctccc gctcggctcc gcgctcgggc
     8101 agaccgtcgg gaacacgctc gaggtggtcg tcgcgacgct gctgatgcac cgcctggtgc
     8161 ggcgcggctc tccgctcaac agcatcagcg gcctcgccgg catgttgatc gccatagctg
     8221 tcggtaccgc ggtgagcgcg accgtcggcg cgctttcgct gcagctcggc gatgtgatcc
     8281 cgctgagcgc cgcggcgagc acgtggcgca cgtggtggct cggcgacgcg tcgggggcgc
     8341 tgatcatcgt gccgctggcc atcgcgtggc accgcccgcc gcctcgcgag tggtggacgg
     8401 gccgtcggct cgagctgacc ctgatgctta ttgcggtcgc cggcctgagc gagctcggcc
     8461 ttcgaaccga gcaaccgctg agctacctcg tgttccccgc gctcatctgg gctgcgctgc
     8521 gctttgggca gcgcggcgcc acactggcga tagcggtggc gaccggtttc accgtgtggg
     8581 agacgaccca ctacgtgggg cccttcgtct acgactcgat cacccgcagc gtgctcgtca
     8641 cgcagctgtt catcgcggtg tccgcgatct ccacgctgtg cctcgcggtg gtggtttcgg
     8701 agcgcgaggc cttcgccgag ggtctgcgcg cgtcgcgtgc ccggatcgtg gaggcggcgg
     8761 acaccgagag gcggcgcctc gagcacaacc tgcacgacgg cgcgcagcag cgactgacgg
     8821 cgctaacggt gcgactcggc ctggcgaccg agagagtgct cgagcatccc gagatcgcgc
     8881 ggtacgtgct ggcggacgcg gagggggagc tcgcgatagc aatcgacgag ttgcgcgacc
     8941 tcgcgcacgg catacacccg acgctgctca cgcagaccgg cctcgcgaac gccatccgcg
     9001 acctggccga gcgctccgcg gttcccgtgc ggctgatcca gctgccgccc acgcgggtgg
     9061 acgacgctgc ggaagccacc gcgtactacg tcgtcgcgga gagcctcgcg aacgcccaga
     9121 agtactcgcg ggcctcgcgg gtcgacatac gcgccgcctt gacgcccggc ctgctccaca
     9181 tcctcgtcgc tgacgatggc atcggtggtg cagacgagag cgccggcacg gggctgcagg
     9241 gccttcggga ccgcgtggag gcgatcggcg gaagcttcgg cgtcgagagc agcgccagcg
     9301 ccggcacgcg aatcgcagcg gtcatcccgg caacgcggct gctgtcctag tcgctcgacc
     9361 ctttcacgcg gcaggggcgt ggcggttacg gctgcgctgc ggtcgagagg gttccgagct
     9421 cctcggccac tgcttctgcg tccgactcgg tccctgccgc cagtacgact tgctctccgc
     9481 cacgagcgag atgtcgcgcc agcggctcgc cgatgcgacc gacaccaatg atcgcggtcg
     9541 tcatcaattc ctccggtcgt gacgttggcc ggaaagtacg cagcttcagt gagaaccaga
     9601 tgaggcctcg tgccagattc gccgtagcag ccgggtcgag atctcctgct ttcgcacaaa
     9661 cgccgcggcg ccggtcgagc cgaggaccgt ttggtccggc gcctcgagcg acacgagcac
     9721 aaccaccgtg cccgggcggc gagccatgat cagcccagcc gtctcgatgc ccgtgatacc
     9781 gggcatgcgc ttgtccacga tcacgaagtc aggcgcgagc tcttcgaggg acgagagagc
     9841 ttcagccccg gatgtcgcct cgcccaccag ctcgaactcg gggatggcgt cgatgagctc
     9901 gcccacgact cttcggaacg gctcctggtc gtccacgacc atgacgccca ctagtccatc
     9961 cgcgcccacc gcggaatcgt cgcacgcctg cgctacgcgg cgagcttgcg gcagatctcg
    10021 gcgagcgagc gcttctctgt ctcgtcgagc gcgccgaata cccggctcac ccgctcggtg
    10081 tgatgaggga agactcggct gacgagctcg aggccgtccg gcagaatccg cacgagtgcg
    10141 gcgcgccggt cgtcgatcat gcgccgccgg cgaacgagac cgcgacgctc gagcgttgtg
    10201 acgacttcgg tcgcgttcgc cttgctccag ccgagccggc gccggagcgt gcgcagctcg
    10261 agctcgcctc ccgcggtcgt gaggaccacg agcgccgaga agcctgcggc cgagatcccc
    10321 tcgcgctcga gctcggcggc tagggtgcgc cgaacgctgg attcggcgcg cacgagcgcc
    10381 tccagggcgc gatgggcaag tgcgtcaccg atctctagcc tcgacacagg cggatcgtag
    10441 gtacgtgtgc ggacgcgaca tccgtcactt gggggcaggg gcgcaaccaa actcgccgta
    10501 ctgagttacg gacctgatga tcattcgaaa gagagtcaca aacagcacga gcccgcgccg
    10561 ggtgctcgcg gccgccgccg cggtcacggc cgtcgctgcg agctccctcg ccggtgccgg
    10621 aggcgcccag gcggcacgcg gcatggaggt cgccgttcag gacgacgccg tcttcgtgaa
    10681 caactcgtac tacgggcgcg acaacgcgct cgacaaggcc caggcgctcg gggcgacgcg
    10741 catccgcgtg aacgcgatct ggtcgcgact cgcgaagaat ccgaactcat cgcgccggcc
    10801 gagcaaacgc gagtaccact gggccgcgat cgacgacctg atcgactcgg ccgccgcgcg
    10861 cgggatccgc gtgcagctca gcctcaccgg gccggtgccg cgctgggccg ccggcgatca
    10921 ccgcgtaggc gtctacaagc cgaaggctca cgagttcgcg ttgttcgcca acgacgccgc
    10981 gcggcatttc aagggccggg tggaccgcta ctcgatttgg aacgagccga attggatcgg
    11041 ctggctgaag ccgattgcgt caggcccgcg catgtaccgc gcgctgttcg tgagcggtta
    11101 ccgcgcgatc aagagcgccg acccgggcgc caaggtgctg atcggcgaga ccgccccgcg
    11161 caagcgcggc cggctgtcga cgggtccact cgcgtttctg cgcggcgtga cgtgcaccga
    11221 cgcgaactgg cgccggcacg gctgcagctc gctgaaggcg gatggctacg cgcatcatcc
    11281 gtacgcgtat gaggtctcgc cgacgaaggt gaccggcggt cgcgacgatg tgacgatggc
    11341 gactctcggc cgtctcacgc acgccctcgc gaagctgcgc gccaaccgcg cgctgcggac
    11401 gccgggcggc gcgtcgctgt acctcaccga gtacgggtac ttcacacacg ggcgccactc
    11461 cctcggcacg cgcaggacct cggcctacct caaggccggc ttccggctcg ggctacggaa
    11521 ccgcgcggtg aagcagatgc tgcagtacat gctcgtctcg ccgcccggag gggcgaactg
    11581 ggacacggcg ctgctcgcca ccaacggcaa cgcgcggagc gcgtacttct ccctgaaggc
    11641 gtggacgaac gccgcggcgg cgcacaacat gatcgcccgg ccgagcgggc cgatctacct
    11701 gccgccgcgc cccgcgagct aggcgcgagc gctcaggcgc gcgaccggtt cgaccgcctg
    11761 ctgcccggca gcgggtgcgt cgagccggcg cccctccacg agcgccttca tgttcgccgt
    11821 cgcctcgccg aggcgggagc cgagcgacgg gatcgagtgc gtgccggaga ctggcatggc
    11881 gtcgcggtac tcgtagatgc gcgcgtggga gtcggcgtgc cggacggagt acacgagccg
    11941 gccgaccacg tcgagggtcg cgcctgcgcc gcacaccacg atcgcggtgg gatcgagcgc
    12001 gcggagagcg cgggacacac gctcctgagc gaggcccatc gagagcagca gcacgcggaa
    12061 accggcgcgg cgaagcgcga gctcgagcgc ctgcacatga atcgcctcga cgtcgagcat
    12121 cgggctcgag tcgaacagca gcacgccctc ctggcgcgtg gccgggggcg cgacgcggcg
    12181 catggcgtga agccagccgg tggcccagcg gcaggcgagc tcgtactcgg cctcgcggcc
    12241 ctcgcggtca cgcgcgatct cgagcgccgg gaggagcagg tcctcgatcg tccgctcgac
    12301 cgagcgcacg gcgaggctct cctccatcgt gcggtcggcg acgctctcgt cgaagcggtc
    12361 gaacgactcc accaggcgcg cgggcgatgt cggcccctcg cctcgctggc gcgcgacctc
    12421 gatcgccgac gagatgttgt gcgtctcgag cagcgcgcgg cggagcgcct cgagctccgc
    12481 gagctcgtac tgacggtgac cgccactcgt gcgccgtggc ttgggatagc cgaagcggcg
    12541 ttcccagctg cgcagtgtgt tcgggctcac gccaagtagc tcggcagccg cgttcgttct
    12601 gattccactc atgtgaaagc cccctggtcg gtcgattgcg accggtcccc tgccgccaga
    12661 ctgcggaggt tttcgcgctt tttccgaacg gacacaagcg ctgaacccga tcttgcactg
    12721 cttccgccct cgatatcggc gcagtccttc acaacgccgc tgggcgggtt gttgcagtgg
    12781 acgggtgttc tacagggaag ggcccacgag ccgcatcggt tcgagtgcga ccagcgcgcc
    12841 gcgaggcggc ccgcacgggg tcacgcgtgc ttcgagccgg ccgaacacgg gccggtcgcc
    12901 gcggatcagc cggatcgcga cgagcgcgac gccgcgcgcg ccgttggcgg cgcgggccac
    12961 gcgcactgcg agctcgtcgt cgctctcggg gctcgtcatc acacgcagca ggttggttcc
    13021 gatcaggtcg ggctcgggcg aaccgaacag ccgctcggcc gcctcgctca ccgcactgat
    13081 gcgcaggtcg ctcgtcacta cgaggaacga catgccctcg gcaggcagcg cctcccgcga
    13141 cgccgcgagc agtaccccca tgccacagtc agagcaaact ctctcagggc gcctgtcgcc
    13201 gcgcggcgcg cgctcctcgc gaacgttgcc gcagcgggag cagaagcgaa tgcgatcgtt
    13261 cggcgaacgc accgcctgga ggaacgggcg tcccccatga gccaattggg tggtcacgag
    13321 tctggcgctc ctttgaatgc ctgccgaggg tccgtcctcg gttgtggagc gactagaaca
    13381 tggctcgcta tcagcgcgtt agcgcgcgcg cgtcaatgca tcgtttcgcg gccgaagtcc
    13441 gtgcgttcga tcgcccgaaa cggcctcctg gggcgcccgt gcacggctct tgcggcgaac
    13501 tggacgcaat cgctggccgc gggtgcgtgt ccagggatta cgatcgacgg cgtgcgcggg
    13561 ctcgtgctcg tatgcgggct ggccgccgcc gcacttgcat cgggatgcgg gggcgatcac
    13621 agcctgccgg ccgcctgccg tgagggcagc ggtgccgtca cgcgcgcact cagcgccgcg
    13681 ccgggacgcg tccaacttgc ggacggcgtg ccgctgtcgc gatgcgtcag gcgcgccg
//
LOCUS       DASVXS010000106         7193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_453891, whole genome shotgun sequence.
ACCESSION   DASVXS010000106 DASVXS010000000
VERSION     DASVXS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453891"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..3297
                     /locus_tag="VF752_05990"
     CDS             70..3297
                     /locus_tag="VF752_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX8855135.1"
                     /translation="MASAELDGDRWLLEPANEPLSLVRADPGDFVLRLRRQRDHELTA
                     EIALRPRLARGFGVFRDRCAARAVEVEIASASDTPSARRLAERTGLRLHIGDTADRVR
                     ELQQEGSLVSVLGDGAHAAAAFDASDLAVGLSSGVSGRFAARADLLAPGLEAVGAVVE
                     AGARRDLAVRDGAAVAVLSGAGGATWGALRTIPFKVATRPDHLGGVAAISDSLVRLRG
                     GRPARTVTQRLSDPQPERWGREGAEEVLAHLHSRPRGLTSEEARERWQRPPDEAEERG
                     LVGLMAEQVKSPLVAVLAAGAGMSVALGAMGDVAMIGAVVVANAFVGAWEEGRAGQAT
                     RALRETSARTARVLRDGDERVLAAEALVPGDVILLASGDRIAADARLLESDSLEVDEA
                     SLTGESLPVPKSADNGGASSRVVLDGTDVTTGTGRAVVVAVGDETRMGAIAAALADSG
                     DRQTPLDARLGRILWRALPLIAVGGGIVTLAGVLRGQPAMQQIALGASVAIAAVPEGL
                     PLLAGVASAGVARRLAKRRALVTRLAAVEALGRVDVACIDKTGTITTGRPAVTLVVAA
                     GGETGSPPDLDPELADVLRTAAKASPSPDAADRNAHPTDTAVLDAARLSGLTEDLPPR
                     DTEARFEPTRGFHATLADRRLSVKGAVEVLAPRCTRVRLEGREQRLTRAERERLTARA
                     ERLAGEGLRVLLVAETHADVPADQPTDLTALGFVCISDPLRPTAAAAIVRCREAGVRV
                     VMITGDHPATADAIARAVGLPYGPERLLTGAEIASLDDDALRERLERATVIARSTPLD
                     KLRIVELLRSAGRVVAMTGDGVNDAPALRLADVGVAMGDGGTEVAREAADLVVTDDDF
                     STLAEALVEGRAFWKNLRRALGLLLGGNAGEVGLMTAAGIAGLPSPLTTRQVLTVNLV
                     TDVLPAVSVAVQPPEHRNLADLRREGGVALDAPLRNDIIRRAVATAAPSVAAYVAASR
                     RMPAAAARSVAYTSIVATQLTQTVDLGWAEGRLTSSVLAAVGGSVAVLAATLTVPWLR
                     SFLGFAPLPASGLALATAAALAAPAVGRAAPVERFFGVPLSGAAA"
     gene            complement(3278..4078)
                     /locus_tag="VF752_05995"
     CDS             complement(3278..4078)
                     /locus_tag="VF752_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX8855136.1"
                     /translation="MAGEEPLGAAFFDLDKTLMEGSSAFQFGRAAYQAGLMSRRQLAR
                     DVWANIHFRLAGTTDEKSDALRERVLDALAGQRVKDLERLGSQVLAGILPRVYPEMLQ
                     VAYEHQDAGRPVFIVTAASNELAELLAHVLGFDGGIGTRAEIKDGVYTGRPEGPFVYR
                     DGKPTAIRELAEKRGIDLERSYAYSDSESDLPMMRAIGHPVAVNPDGPLEAVAREQGW
                     QIMRFDKLARRLRIAAAISTVAVLGGGGGYVAARARQRPQGRLRLPRR"
     gene            complement(4088..5272)
                     /locus_tag="VF752_06000"
     CDS             complement(4088..5272)
                     /locus_tag="VF752_06000"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8855137.1"
                     /translation="MPKTVILGTARTPFGRLGGGLSSLDATDLGGTTIAAALERAEVK
                     PEQVQHVVFGQVLQAGQGQIPSRQAQIKGGIPREVSSETVNKVCASGLRAAGMVDQSV
                     RAGDLDVAVAGGMESMSGAPYLLKQARFGYRMGDAKALDAMVHDGLTNPFTDKQMFQE
                     ATEVGDELEMTRADMDKFALRSHELAIQATDEGRLAEEIVPVTVKSKKGETTVEVDEA
                     PRRESSLEALSKLPGLAGKDGSHTAGNSPGVNDGAGAMVLSSDEWARSNGAKPIATIV
                     AQAAVADDFPYLARTPANATKAALEKAGVSIDDVDLFEINEAFASVALNSMRMLGIDE
                     SKVNVNGGAIALGHPLGASGARIIGALVHELRRRGGGLGVAALCSGGGQGDALVIEVN
                     GN"
     gene            5359..6801
                     /locus_tag="VF752_06005"
     CDS             5359..6801
                     /locus_tag="VF752_06005"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX8855138.1"
                     /translation="MGEIRVNARGGASGQTDADTRVVGLFEGEALDTPALQALVEAGE
                     AKPRLKSVAVAHDGGTRVLIAGLGKRDEFDAEKARVAAASAVAKAKELATRSLSWEAP
                     TDDPRHAAALVEGTLLRLYEFNQYKSNRDDDENNRTAVESLEIVGDGDLDAAVNEARV
                     RALAQNAARDLQNQPSNVATPEYLANRARELAAAHDALSFEAFERDQIREMNMGAFSA
                     VAQGSDRSPRLIVLRYEGGGSGPHLGFVGKAVTFDTGGISIKPAGKMHEMKFDMSGGA
                     AVIEAMGAIAQLGVPARVTAVVPATENMPSGHAMKPGDIVTAMNGKTIEINNTDAEGR
                     LILADALAYAVDQGAERIVDLATLTGAIVTTLGSTYCGYFSNDDDWYARVEAAGKATG
                     EEGWRLPLHPEYFELTKGQYADLTNSSEARKAASIYAAEFLRQFVDGRPWVHFDIAGV
                     AWGMTRPYVGSGASGFGVRTLIELAETAGG"
     gene            complement(6807..>7193)
                     /locus_tag="VF752_06010"
     CDS             complement(6807..>7193)
                     /locus_tag="VF752_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855139.1"
                     /translation="VRVGPDGRAAIRSTAHGGWRWCKQRRCEGVWGEYSGWSRVSRGS
                     HAGHIPLDSKRHSVRFGGGLPLVHGKSYSYEPLYPGAGLNERTTAAPSLRLVPLEGVR
                     DRRDAFPDISPPWRKRAWSHPESGSS"
BASE COUNT         1010 a         2525 c         2602 g         1056 t
ORIGIN      
        1 gcgggatcgc cttggggcat cctacggcgc ggccggctga ctgccgagga cggaaccttc
       61 gacgggcgag tggccagcgc tgagctcgac ggcgatcgct ggctgctcga gcctgccaac
      121 gagccgctgt cgctcgtgcg cgctgacccg ggcgacttcg tgctccgcct ccgacggcag
      181 cgggaccacg aactcacggc cgagattgcg ctccgtccgc gcctcgctcg cggcttcggc
      241 gtgttccgcg accgctgcgc ggcgcgcgcg gtcgaggtgg agatcgcgag cgcatcggac
      301 accccgtccg cgcggcggct ggccgagcga accggcctgc gccttcacat cggggacact
      361 gcagaccgag tcagagagct gcagcaagaa gggtctctcg tgagcgtcct cggggacggc
      421 gcgcatgccg cggcggcgtt cgacgcgtcg gatctcgcgg tcggcctgag ctccggcgtc
      481 agcggacgct tcgccgcccg agcggacctg ctcgcgccgg gactcgaggc cgtgggtgcg
      541 gtggtcgaag ccggcgcccg gcgcgacctg gcggtacgcg acggtgctgc cgtcgccgtc
      601 ctctcaggcg ccggtggagc gacgtgggga gctctcagga cgattccgtt caaggtcgca
      661 acacgtcccg atcaccttgg cggagtcgcg gccatctccg acagtctcgt gcgactacgt
      721 ggcggccgac cggcccgaac cgtgacccaa cggctcagcg atccgcagcc cgagcgctgg
      781 gggcgcgagg gcgccgagga ggtgctcgct cacctccaca gccggccccg cggcctgacc
      841 agcgaggagg cgcgcgaacg gtggcagcga cctccggacg aggccgagga gcgcgggctc
      901 gtcggcctca tggctgagca ggtcaagtca ccgctcgtgg ctgtgctggc cgcgggcgcc
      961 gggatgtccg tcgcgctcgg tgcgatgggg gacgtggcga tgatcggagc ggtcgtggtc
     1021 gcgaacgcgt tcgtcggcgc gtgggaggaa ggccgtgccg ggcaggcaac gcgggctctt
     1081 cgggagacgt cggctcgtac ggcgcgcgtc ctccgcgacg gcgacgaacg ggtgctcgcg
     1141 gcggaggcgc tcgtgccggg cgacgtgatc ttgcttgcct ccggggaccg gatcgcagcc
     1201 gacgcgcgct tgctcgagtc cgattcgctc gaggtcgacg aggcctcgct gaccggcgaa
     1261 tcgctgcctg tgccgaagtc ggccgacaac gggggcgcgt cgtcgcgcgt cgtcctcgac
     1321 ggcaccgatg tgaccaccgg gacggggcgg gcggttgtgg tggctgtagg cgatgaaacg
     1381 cgcatgggcg ccatcgcggc tgcgctcgcg gacagcggcg accgccagac gccgctcgac
     1441 gcgaggctcg ggcgaatcct atggcgtgcg cttccgctca tcgccgtcgg tggcgggatc
     1501 gtgactctcg cgggcgttct ccgggggcag cctgcgatgc agcagatcgc actcggagcg
     1561 agtgtcgcca tcgcggcggt cccggagggt ctgccgctcc tcgccggggt cgccagcgcc
     1621 ggtgtggccc gccggctcgc gaagcggcgc gcgctggtca cgcgcctcgc ggcggtcgag
     1681 gcgctcgggc gcgtggacgt cgcgtgcatc gacaagaccg gcacgattac gacgggacgg
     1741 ccggccgtga ccctcgtcgt ggctgccggc ggcgagacgg gctccccacc ggacctggac
     1801 ccggagctcg ccgacgtgct caggaccgcg gcaaaggcga gcccgtctcc tgatgcagcc
     1861 gacaggaacg cccacccaac cgacacagcg gttctcgacg cggcacggct ctccggcttg
     1921 accgaagatt tgccgccccg ggataccgag gctcgcttcg agccaacccg aggcttccac
     1981 gccactctcg cggatcgccg gctgtccgtg aagggcgcgg tggaggtgct ggcgcctcga
     2041 tgcactcgcg tgcggctgga gggacgcgag cagcggctca cgagggccga gcgcgaacgc
     2101 ctcacggcgc gcgcggagcg gctcgcgggc gagggcctgc gggtgctact ggtcgccgag
     2161 acgcacgccg acgtacccgc ggaccagccg actgacctca ctgcgcttgg gtttgtctgc
     2221 atcagcgatc cgctccgccc caccgcggcc gcagcgatcg tccggtgccg cgaagcagga
     2281 gttcgcgtgg tgatgatcac cggcgatcac ccggccacgg ccgacgcgat cgcccgcgcc
     2341 gtagggcttc catacgggcc ggagcgcctg ctcacgggcg ccgagatcgc gagcctggac
     2401 gacgacgcgt tgagggagcg tctcgagcgc gccacggtga tcgctcgcag cactccgctc
     2461 gacaagctgc gaatcgtgga gctgcttcgc tccgcggggc gcgtcgtggc gatgacgggc
     2521 gacggcgtga acgacgcccc cgccctgcgg cttgccgacg tgggcgtggc gatgggcgat
     2581 ggcggcacgg aggtcgcgcg cgaggccgcg gatctcgtcg tgaccgacga cgacttctcc
     2641 actctcgccg aggccctcgt ggagggccgg gcattctgga agaacctgcg ccgcgcgcta
     2701 ggtcttctcc tgggcgggaa cgccggggag gtcggcctga tgaccgcggc gggcatcgcg
     2761 gggctgccgt cgccactcac cactcgccag gtgctgaccg tgaacctggt gacggacgtt
     2821 ttgcccgccg tctcggtggc agtgcagcca cccgagcatc gcaatctcgc cgaccttcgg
     2881 cgcgagggcg gcgtcgccct cgacgcgccg ctgcgcaacg acatcatcag gcgcgccgtg
     2941 gcaactgctg cccccagcgt ggccgcgtac gtggcggcgt cacgcagaat gcccgcggca
     3001 gcggcgcgca gcgtcgccta taccagcatc gtcgccaccc agctgacgca gaccgtcgat
     3061 ctgggctggg ctgaggggcg cctgacgagc tcagtgctcg cggccgtcgg tgggtctgtt
     3121 gccgtgctgg cggcgaccct cacggttccc tggttgcgca gcttcctcgg tttcgcgccg
     3181 ctgcccgcct ccggcctcgc tctcgcgact gcagccgcgc tggccgctcc ggcggtgggg
     3241 cgcgctgcgc ccgtcgagcg cttcttcggt gtgcccctca gcggcgcggc agcctgagtc
     3301 tcccctgcgg ccgctgacgc gcacgtgcgg ccacgtagcc tccgccaccg ccgagaaccg
     3361 cgacggttga gattgccgcg gctatccgca ggcggcgcgc gagcttgtcg aagcgcatga
     3421 tctgccagcc ctgctcgcgc gcgaccgcct cgagcgggcc atccgggttc acggcgaccg
     3481 ggtggccgat ggcgcgcatc atcggcaggt ccgactcgga gtcggagtac gcgtaggacc
     3541 tctccaggtc gatgcctcgc ttctcggcta gctcacggat cgccgtcggc ttgccatcgc
     3601 ggtacacgaa gggaccttcc gggcgccccg tgtacacgcc gtccttgatc tccgcgcgcg
     3661 tgccgatacc gccgtcgaat ccgagcacat gggcgagtag ctcggcaagc tcgttagacg
     3721 ccgcggtgac gatgaacacg ggccggcctg cgtcctggtg ctcgtaggca acctgcagca
     3781 tctccggata gacgcgcggg aggatgccgg caagcacctg cgacccaagg cgctcgaggt
     3841 ccttcacgcg ctggcccgcg agcgcgtcca gcacgcgctc gcgcagcgcg tcgctcttct
     3901 cgtcggtcgt gccagcgagg cggaagtgga tattcgccca cacgtcgcgc gccagctggc
     3961 gccggctcat gagccccgcc tggtaggcgg cacggccgaa ctggaaggcg ctcgagccct
     4021 ccatgagggt cttgtccagg tcgaagaacg ccgcgcctag cggctcctcg cctgccatag
     4081 gcgactccta gttgccgttg acttcgatga ccagcgcgtc gccctgaccg ccgccggagc
     4141 agagggccgc gacgccgagt ccgcccccgc gacggcgaag ctcgtgcacg agcgcgccga
     4201 tgatgcgcgc gccgctcgcc ccaagtgggt gaccgagcgc gatcgcgccg ccgttgacgt
     4261 tcaccttcga ctcatcgatc ccgagcatcc gcatcgagtt gagagcaacc gaggcgaacg
     4321 cctcgttgat ctcgaagagg tcgacgtcgt cgatcgagac gccagccttc tcgagcgccg
     4381 ccttggtcgc attggcggga gtgcgggcca ggtacgggaa gtcgtccgcc acggcggcct
     4441 gggccacgat cgtcgcgatc ggcttcgcgc cgttcgagcg cgcccactcg tcggaggaca
     4501 gcaccatcgc gccggcgccg tcgttgacgc ctggcgagtt gcccgcggta tgcgacccgt
     4561 ccttgccggc gaggccgggg agcttcgaga gcgcttcgag cgaggactcg cggcgcgggg
     4621 cctcgtcgac ctcgaccgtg gtttcgccct tcttcgactt gaccgtgacg ggcacgatct
     4681 cctcggcgag gcggccctcg tccgtggcct ggatggcgag ctcgtggctg cgaagcgcga
     4741 acttgtccat gtccgcgcgc gtcatctcca gctcgtcgcc cacctcggtg gcctcctgga
     4801 acatctgctt gtcggtgaac gggttggtga ggccgtcgtg caccatcgcg tcgagcgcct
     4861 tggcgtcgcc catgcgatag ccgaagcgcg cctgcttgag caggtacggc gcgcccgaca
     4921 tcgactccat gccgccggcg acggccacgt cgaggtcgcc ggcacgcacc gactggtcga
     4981 ccatgcccgc cgcccgcagc cccgaggcgc agaccttgtt cacggtctcc gacgagacct
     5041 ccctggggat gccgcccttg atctgcgcct ggcgcgaggg gatctgaccc tggcctgcct
     5101 gcagaacctg cccaaagacg acgtgctgca cctgctcggg ctttacctcc gcgcgctcga
     5161 gcgcggctgc gatcgtcgtg ccgcccagat cggtggcgtc gagcgatgag aggccgccgc
     5221 cgagcctgcc gaagggtgtg cgcgcggtgc cgaggatgac tgtcttgggc atgtgttcgg
     5281 atcgtctccc tggtgcgttg acgagcgaac cttagcgatg gtttgcgtcc ggtcgcgcac
     5341 ggttacactg cccgctcgat gggagagatt cgtgtgaacg ctcgcggggg agcgtcggga
     5401 cagaccgatg ccgacacccg cgtggtcggg ctcttcgagg gagaggcact tgacaccccc
     5461 gcgcttcagg cgctcgtgga agcgggcgaa gccaagccgc gtctgaagag cgtggccgta
     5521 gctcacgacg gcggcacgcg agtgctgatc gccggactcg gcaagcgcga cgagttcgac
     5581 gctgagaagg cgcgcgtggc ggccgccagt gccgtcgcga aggcgaagga gctggcgacg
     5641 cgctcgctgt cgtgggaggc gccaaccgac gacccgcgcc acgccgcggc gctcgtcgag
     5701 ggcaccctgc ttcggctcta cgagttcaac cagtacaagt ccaaccgcga cgacgacgag
     5761 aacaaccgca cggcggtcga gtcgctcgag atcgtgggcg acggcgacct cgatgcggcc
     5821 gtgaacgaag ctcgcgtgcg cgccctcgcg cagaacgcag cgcgcgatct gcagaaccag
     5881 ccctccaacg tcgccacgcc ggaatacctg gcgaaccgcg ctcgagagct tgccgcggcg
     5941 cacgacgcgc tcagcttcga ggccttcgag cgcgaccaga tccgcgagat gaatatgggc
     6001 gccttctccg cggttgcaca gggaagcgac cgctcgccgc gcctgatcgt gctgcgctac
     6061 gagggcggcg gcagcgggcc gcacctcggc ttcgtgggca aggccgtgac cttcgacacc
     6121 ggcggcatct cgatcaagcc cgcgggcaag atgcacgaga tgaagttcga catgtcgggc
     6181 ggcgcggccg tgatcgaggc gatgggcgcg atcgcgcagc ttggcgtgcc tgctcgcgtc
     6241 accgccgtcg tgcccgcgac cgagaacatg ccgagcggcc acgcgatgaa gccgggcgac
     6301 atcgtgacgg cgatgaacgg caagacgatc gagatcaaca acaccgacgc cgaggggcgg
     6361 ttgattttgg ccgacgccct cgcctacgcg gtcgaccagg gcgccgagcg gatcgtggac
     6421 ctcgccacgc tcaccggcgc gatcgtgaca acgctcggct cgacgtactg cggctacttc
     6481 tcgaacgacg atgactggta cgcccgcgtg gaggccgccg gcaaggcgac cggcgaggaa
     6541 ggctggcggc tgccgctgca ccccgagtac ttcgagctca ccaaggggca gtacgccgac
     6601 ctgacaaaca gctcggaggc gcgcaaggcg gcctcgatct acgcggccga gttcctgcgc
     6661 cagttcgtgg acggcaggcc ctgggtgcac tttgacatcg ccggggtggc gtggggcatg
     6721 acgcgcccgt acgtgggcag cggcgcttcc ggcttcggcg tgcgcacgct gatcgagctg
     6781 gccgagacgg ccggcggcta ggcagctcac gagctgcctg actccgggtg cgaccaggcg
     6841 cgcttgcgcc acggcgggct gatgtcgggg aacgcgtcgc gccggtcgcg cacgccctcg
     6901 agcggcacga ggcgcaggct cggtgctgcg gtggtgcgct cgttgagccc ggcgcccgga
     6961 tagagcggct catagctgta gctcttgccg tgcaccaacg ggagcccgcc gccgaaccga
     7021 acgctgtggc gcttcgagtc gagcgggatg tggcccgcgt gactcccgcg cgagacccgc
     7081 gaccagccgc tgtactcgcc ccagaccccc tcgcagcggc gctgcttgca ccagcgccag
     7141 ccgccgtgcg cggtggagcg gatcgccgcc ctgccgtccg gcccgacgcg cac
//
LOCUS       DASVXS010000107        12258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_580210, whole genome shotgun sequence.
ACCESSION   DASVXS010000107 DASVXS010000000
VERSION     DASVXS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12258
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580210"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF752_06015"
     CDS             <1..247
                     /locus_tag="VF752_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855140.1"
                     /translation="TGPVHVKPKFVKPGQRFTVTITDANVRQATVSLTGVSNDPIQAD
                     ATNRTATLTVPATASCGNKLVTVEGDVSAQAYVGVGR"
     gene            complement(226..816)
                     /locus_tag="VF752_06020"
     CDS             complement(226..816)
                     /locus_tag="VF752_06020"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8855141.1"
                     /translation="MKIGIVVFDGFDELDAIGPYEVFRNAGWATDLVTLEPQERVHGS
                     HGLVIVPHAVLGEGYDLVVVPGGGWNDRGEQGAWAEAQRGDLPAALRALHERGARIAS
                     VCTGGMVLAAAGITAGRPSVTHHHAIDELRSSGAEVVDARVVDHDDLVTSGGVTSGID
                     MALWIVERELGPEAAAAVADEMEHDRRGEVYRPTPT"
     gene            complement(813..1940)
                     /locus_tag="VF752_06025"
     CDS             complement(813..1940)
                     /locus_tag="VF752_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX8855142.1"
                     /translation="MTEGVAEILSGRSICVCAGSGGVGKTTTAAAIATGMAAQGLKVA
                     VLTIDPARRLANSLGLPELGNEERRVDPAVLEAAGLGGDGELWALMLDAKRTFDELVE
                     RHAPDERSRDAILANRVYQELSNAVAGSQEYMAMEKLYELHHEQRYDLLVLDTPPTRN
                     ALDFLDAPERLSRFVDSRSLQFFLKPSRTGLRLLGRSTGLMFSVLKRVTGVDMLQDLS
                     EFFQSFGDMAEGFSERARRVNEMLADPRTVFFVVTSPRGAAVDEAVFFHRRLRERHMP
                     SGGVIVNRMTMIEDPGDDDVEDDLAALLGEGLGRKVARNFDDYRDIARGDRANLERLE
                     RELGRDPMILVPHLEDDVHDAAGLARMNEYLFAERGSRVPA"
     gene            complement(1937..2866)
                     /locus_tag="VF752_06030"
     CDS             complement(1937..2866)
                     /locus_tag="VF752_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX8855143.1"
                     /translation="MSSLLDKRLIFVTGKGGVGKTTVATALGLLAARAGKRTIVCEVA
                     KQERMSRVFRREGVGYHETELAERLFAISIDPEHAMEEYLRQRVPGPLYQLLFENRLF
                     QYFAAAAPGTREFLTIGKVWELAQLERRTAGARPYDFVILDAPATGHGLGMLHTPATF
                     RDVARAGPIQNHADRVDRFVRDPRQTGVLAVALPEEMPVNETIELEQALHTRMGMELD
                     AVVANALYPERFSGEEGEQIEAADGTHPVPAVAAALNAAHAEHVRARGQRSQLRRLRR
                     ASAANVATLPFLFEPELSLDSLERLSLELERKL"
     gene            2996..3778
                     /locus_tag="VF752_06035"
     CDS             2996..3778
                     /locus_tag="VF752_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8855144.1"
                     /translation="MTREPDKPGAKVIAFANQKGGVAKTTTTLNLAAAFVEKGHRALC
                     VDMDPQANLTMSQGIDPDTLESSMYDVLVHHISIREVIRRREVDIACASIDLAGAEIA
                     MSTQIGRERSLEKAFAPIKDDYDFICIDTPPSLGLLTINALTAADKVIVPVQCEYLSM
                     RGLIQLQNTLSMIRENLNPDVEIEGILPTLVDTRTMHAKEAIELLEENFGDRVFASRI
                     KKTVRFAEAPVKGMSVLKYDPDGKAAYAYRQLAEEVLANGTR"
     gene            3765..5192
                     /gene="ftsZ"
                     /locus_tag="VF752_06040"
     CDS             3765..5192
                     /gene="ftsZ"
                     /locus_tag="VF752_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8855145.1"
                     /translation="MERGKRASMREGPLSALFRRTEEEGIGDTPAPREEKPREEEPRR
                     EPSKEQLRPAPPSAREPQLTREARAVPSPEERLRAVFSSDIPENILDKPSQSPQPQPT
                     SEWVPAGDPTRGPVLRVVGVGGAGVNAVNRMIEAGVEGVDFIAVNTDAQSLQSSEAYE
                     TVHIGAEVTRGLGAGSNPELGRQSALEDYDHLKSLLKGADMVFIAAGAGGGTGTGAAP
                     VIARISREVGALTVGIITKPFSFEGSRRALQADQGVEELAREVDTLITIPNSRLLSVL
                     DKQTSMVEAFRVADDVLRQGVQGISDLVTLPGLINLDFADVRTIMSQAGQALLGIGMA
                     TGEHRAIEAAQRAVESPLLETSVDGARSILLSITGGRDLSLWEVNEAARAVSEAAHPD
                     ANIIFGAMVDEKVVDQVWVTVVATGYGDRPVRRAAPRAESADRTERFERPRRDAREAD
                     HEPRVTRTRVSSLGELDVPEFIPRA"
     gene            5335..6858
                     /locus_tag="VF752_06045"
     CDS             5335..6858
                     /locus_tag="VF752_06045"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8855146.1"
                     /translation="MKGVVAAGHPLTAQAGADVLRSGGNAVDAAVAAVLMSFVCESPL
                     TGPGAGGFMLVHTPDGEDVLLDFFVAAPGRGLDRLEPAPLMPIDVEFSKEAVQVFNIG
                     PSSCGAYGTTLGLAHALERFGSVPLSALVERPARAAREGVEINDIQSYLHKILGPILS
                     STPEAAAIYEPKGRPLRPGETIYLPALGDLLERLGAEGPGFLYTGDVGAAASNWLLER
                     GGLLTRDDLAAYEPIEREPVRARYHGRDVLTNPPPSSGGILIAHSLNLLERLGRRPDP
                     RTLVGVMARTNRERTEEFLSGLHSEGYLESFLAHEALDSAAREVGSRLGSTTHISVLD
                     DRGRCASVTCSNGSSSGVIVPGTGLHLNNMLGEQDLNPHGYHRHEPGRRLPSMMAPTV
                     VLRDGRPEIGLGSAGSNRIRSAIIQTALASVNDGLPAQAAVEAPRLHLEEAVVDAEPG
                     IDPAGLDALEADGWTVRRWRERNLFFGGVQAVARDPETGALSGGGDPRRGGAAIVVA"
     gene            6960..8402
                     /locus_tag="VF752_06050"
     CDS             6960..8402
                     /locus_tag="VF752_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8855147.1"
                     /translation="MRPRLAPLDGSFLRVETPNAHMHVGWCAVFRPHPDRPRPSVEML
                     RRSVAGRLHLTPRFRQRLAFPPPGFGEPFWVDAPDFDAGSHITALTCDDDAVSFETFH
                     MLTDAALSTPLDRGRPLWHVYLVPRLEDERVGMVAKLHHAMVDGLSAVELGLLLLDTE
                     RDAPLPPPPEPSERWLPSRVPGQISLAVESAASLAGDALRGARTAAAFAFSPRRQTAA
                     LAGTLRRVAAVVRDDLLPAAPDSAVNVPIGPQRTLVRGRVEMDALERARTALRASGGP
                     RVTVNDVYLAAVAGALRTLALRRGERPVPLKAMVPVSVRSDSERASLGNRISFVFLEL
                     PVHKTYPAARLEDVHSATRAFKESGRIAGGVTVLSALGFLPDPLKDIAARMAASARVY
                     NLTISNIPGPGVPVFMLGAELEEAHPVVPLSEDHALSVGVFTYRSGAFFGFYADPDAL
                     PEVSELPAAVAAEVAVLAGQRPAPRRLAVV"
     gene            complement(8540..8776)
                     /locus_tag="VF752_06055"
     CDS             complement(8540..8776)
                     /locus_tag="VF752_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX8855148.1"
                     /translation="MTTVDARGLACPLPLTLARGKMGELEDGDVLVVLATDPEAPIDL
                     AAWAADEGHAFAEREAGGWAELTLTKGGRTSMEQ"
     gene            8788..9201
                     /locus_tag="VF752_06060"
     CDS             8788..9201
                     /locus_tag="VF752_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855149.1"
                     /translation="MNGGTYEIAAVFSSGLHERLYSGLSLVVSTAAEGKRCAALAAFG
                     GLAMFIDRELMQHAQEPDATPSLTWAGRDTFARSLASLRDTALELDTLDVWACAASVE
                     TMGLTESDVQDRGLKGVLSTPRFLREAGAATLLFV"
     gene            complement(9198..9839)
                     /locus_tag="VF752_06065"
     CDS             complement(9198..9839)
                     /locus_tag="VF752_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8855150.1"
                     /translation="MGDEVERLPLFPLGIVLLPEEVVPLHIFEERYKTMITECLEEGR
                     EFGIVWLAPDGLREIGCTAEIAQLLERMEDGRMNILVRGTRPFRLVERIEDMPYPAGD
                     VEMLDDESSAESERDQLAAQARERYADLVEQLTDSRPDETGLEELGAYGMASTIDFAP
                     ELKQDLLEARSESERLRMVGELFVSTMRRLEFVERTSEQARSNGHIKPSSLGP"
     gene            9943..10926
                     /locus_tag="VF752_06070"
     CDS             9943..10926
                     /locus_tag="VF752_06070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8855151.1"
                     /translation="MAHPRPLSTLGVRRRTLPFLGALAVAFAVAPLPAVERDGSALLA
                     AGVLAVAVVAAVGLTPWHRLPAWMQALPPLAVFPVIALLRDADGGTTSGYGVLVILPV
                     FWFAMYGTRRELCIAIAAMAATLIAPLLLAGNPLYPSTEWRRVLLWAVAAPLIGFTVQ
                     NLIARVRGKTEELESAARTDTLTGLPNRRALDEEIPDELERAARYGYPLSLAMIDLDH
                     FKRLNDERGHQAGDRLLKRAAAAWRSELRHSDIVVRYAGDEFLVALPNCTPANAHALI
                     DRLVRALPPGQTCSAGVAHLRSGESVDQLVARADAALLAAKRGGRAQVVSG"
     gene            complement(10923..11840)
                     /locus_tag="VF752_06075"
     CDS             complement(10923..11840)
                     /locus_tag="VF752_06075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8855152.1"
                     /translation="MKAEAAERIKDVNVRYHDVAAETYDTKWGINYDEPGRRQVTAKL
                     QKAIGAPLPHFGRALEIGAGTGYFSLNLLRAGVIGESVATDISQGMLDALSESAERLG
                     LEVETARCEAAKLPFPDASFDLVFGHAVLHHLPDLDAAFREFRRVLRPGGMVAFCGEP
                     SYYGDRIASVPKRAALAAAPAWRRLMRAGIRPNGHVADPADEEEHRLEMVVDVHAFTP
                     ADLYGFARHAGFDQVRVRGEELAASLFGWANRTLESTADPDSVPWAWRQYAYRGYLAL
                     QAVDRALLEPRLPAALFYNLLISARAPGA"
     gene            11888..>12258
                     /locus_tag="VF752_06080"
     CDS             11888..>12258
                     /locus_tag="VF752_06080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8855153.1"
                     /translation="MAHSAAEYVQRDQPDGVPPLELTGERTLPDVPEENYWFRRHLAV
                     YEWIAARCEGLDVIDMACGEGYGTDVLARRAKTVTGVDANPEAHEHACLKYARPGVKF
                     VRDLVETYSEPCDAVVFLQTI"
BASE COUNT         1785 a         4320 c         4292 g         1861 t
ORIGIN      
        1 cactggcccc gtgcacgtga aaccgaagtt cgtgaagccg ggtcagcgct tcaccgtcac
       61 gatcaccgac gcgaacgtgc gccaggcgac cgtgagcctg acgggcgtgt cgaacgatcc
      121 gattcaggcg gacgcgacca accgcacggc aacgctcacg gtcccggcga cggcgagctg
      181 cggcaacaag ctcgtcacgg tggagggcga cgtgagcgcg caggcctacg tgggcgtggg
      241 tcggtagacc tcgccgcggc gatcgtgctc catctcgtcc gcgacggccg ccgccgcctc
      301 gggcccgagc tcgcgctcga cgatccagag cgccatgtcg atgccggacg tcacgccgcc
      361 ggaagtcacg aggtcgtcgt ggtcgaccac gcgtgcgtcg acaacctcgg cgccggatga
      421 gcgcaactcg tcgatcgcgt gatggtgcgt gaccgaaggc cgccccgcgg tgatgcccgc
      481 ggccgcgagc accatgccgc ccgtgcacac cgacgcgatg cgcgcgccgc gctcgtgcag
      541 cgcacgaagc gccgccggca ggtcgccgcg ctgggcctcg gcccaggcgc cctgctcgcc
      601 gcgatcgttc cagccgccgc ccggcaccac caccaggtcg tagccctcgc cgagcaccgc
      661 gtgcggcacg atcacgagtc cgtggctccc gtgcacgcgc tcctgtggct cgagcgtgac
      721 caggtcggtc gcccagccgg cgttgcggaa cacctcgtac ggcccgatcg cgtcgagctc
      781 gtcgaagccg tcgaacacca ctattccgat cttcatgccg ggaccctaga gccacgctcg
      841 gcgaacaggt actcgttcat gcgcgcgagc ccggcggcgt cgtgcacgtc gtcctcgagg
      901 tgcggcacga ggatcatcgg gtcgcgaccc agctcgcgct cgaggcgctc gaggttcgcg
      961 cggtcgccgc gcgcgatgtc gcggtagtcg tcgaagttcc gcgccacctt gcggccgagt
     1021 ccctcgccga ggagcgccgc gaggtcgtct tcgacgtcgt cgtctcccgg gtcctcgatc
     1081 atcgtcatcc ggttcacgat cacgccgccc gacggcatgt gccgctcgcg cagcctgcgg
     1141 tggaagaaca cggcctcgtc aactgccgcc ccccgcggcg aggtgacgac gaagaacacg
     1201 gtgcgcgggt cggcgagcat ctcgttcacg cggcgggccc gctcgctgaa gccctccgcc
     1261 atgtcgccga agctctggaa gaactccgac aggtcctgga gcatgtcgac gccggtcacg
     1321 cgcttgagca ccgagaacat cagccccgtg ctccggccga gcaggcgcag cccggtgcga
     1381 ctcggcttga ggaagaactg cagcgagcgc gagtccacga agcgcgacag gcgctcgggg
     1441 gcgtcgagga agtcgagcgc gttgcgcgta ggaggcgtgt cgagcacgag caggtcgtag
     1501 cgctgctcgt ggtgcagctc gtagagcttc tccatcgcca tgtactcctg cgagccggcg
     1561 acggcgttcg acagctcctg gtacacgcgg tttgcgagga tcgcgtcgcg cgaacgttcg
     1621 tcgggcgcgt gccgctccac gagttcgtcg aaggtgcgct tggcgtcgag catcagcgcc
     1681 cacagctcgc catcgccacc gaggccggca gcctcgagca cggctggatc gacccggcgc
     1741 tcctcgttgc cgagctccgg cagcccgagc gagttggcga gcctgcgcgc ggggtcgatc
     1801 gtgagcaccg cgaccttgag cccctgcgcc gccatcccgg tggcgatcgc agcggccgtc
     1861 gtggtcttgc ccacgccgcc cgagcccgcg cagacgcaga tcgagcggcc ggacaggatc
     1921 tcggcgacgc cctcggtcac agcttcctct cgagctcgag cgagaggcgc tcgagcgagt
     1981 cgagcgagag ctccggctcg aacaggaagg gcagcgtggc cacgttcgct gcgctcgcgc
     2041 ggcgcaggcg gcgcagctgc gagcgctggc cgcgtgcgcg cacgtgctcg gcgtgcgcgg
     2101 cgttgagcgc cgccgcgacc gcggggacgg ggtgcgtgcc gtcggccgcc tcgatctgct
     2161 cgccctcctc gccgctgaag cgctcggggt agagggcgtt cgcgacgacc gcgtcgagct
     2221 ccatccccat cctcgtatgc agcgcctgct ctagctcgat cgtctcgttc accggcatct
     2281 cctcgggcag cgcgaccgcg agcacgccgg tctgccgcgg gtcgcgcacg aaccggtcga
     2341 cgcggtccgc gtggttttgg atcggccccg cgcgcgccac gtcgcggaag gtggcgggcg
     2401 tgtgcagcat gccgagcccg tgccccgtcg caggcgcgtc gaggatcacg aagtcgtacg
     2461 gccgcgcgcc ggccgtccgt cgctcgagct gggccagctc ccacaccttg ccgatcgtga
     2521 ggaactcgcg cgttccgggc gccgcggcgg cgaagtactg gaagagccgg ttctcgaaca
     2581 gcagctggta gagcgggccg ggcacccgct gccgcaggta ctcctccatc gcgtgctctg
     2641 ggtcgatcga gatcgcgaac aggcgctccg ccagctcggt ctcgtggtag cccacgccct
     2701 cacgccggaa aacccgcgac attcgctcct gcttggccac ctcgcacacg atcgtgcgct
     2761 ttccggcgcg cgcagcgagc aggcccagcg cagtggcgac ggtggtcttt ccgaccccgc
     2821 ccttgccggt gacgaagatc agccgcttgt cgagtagcga cgacatgaag aaaagagggt
     2881 agggccgggg aaggattccc catcaatcgt gagaaatacg ggattcgaca tgggtctgtt
     2941 cgaccgaaag agcgccaccc cgagggaaga ggcgcgaaat agatcggagt ccgcagtgac
     3001 acgcgagcca gacaagcctg gggcaaaggt catcgcgttc gccaatcaga agggcggggt
     3061 ggccaagacc acgacgacgc tgaacctagc cgcggcgttc gtcgagaagg gacaccgcgc
     3121 gctctgcgtg gacatggacc cgcaggccaa tctcacgatg agccagggga tcgacccgga
     3181 cacgctcgag tcttcgatgt acgacgtgct cgtgcaccac atctccatcc gcgaggtgat
     3241 ccggcggcgc gaggtggaca tcgcctgtgc gtcgatcgac ctggccgggg ccgagatcgc
     3301 gatgtccacc cagatcggcc gcgagcgctc gctggagaag gctttcgcgc ccatcaagga
     3361 cgactacgac ttcatctgca tcgacacgcc gccgagtctc gggctgctca cgatcaacgc
     3421 gcttacggcc gcggacaagg tcatcgtccc cgtccaatgc gagtatctgt cgatgcgcgg
     3481 gctgatccag cttcagaaca cgctttcgat gattcgcgag aacctcaacc cggatgtcga
     3541 gatcgagggc atcctgccga ctctcgtcga cacgcgcacg atgcacgcca aggaggcgat
     3601 cgagctgctc gaggagaact tcggcgaccg ggtgttcgcc tcgcggatca agaagaccgt
     3661 gcgcttcgcg gaggcgccgg tgaaggggat gtcggtgctc aaatacgacc ctgacggaaa
     3721 ggcggcgtac gcctaccggc agctggcaga ggaggttctc gccaatggaa cgcggtaagc
     3781 gagcgagcat gcgcgagggc ccactgtcgg ccctcttccg ccgtaccgag gaagagggga
     3841 tcggcgacac gcccgcgcct cgcgaggaga agccgcgtga ggaggagccc cgccgcgagc
     3901 cctcgaagga gcagctgcgc ccggcgccgc cgtcggcacg cgagccgcag ctcacgcgtg
     3961 aggctcgcgc cgtgccgtcg ccggaggagc gcctgcgggc cgtcttctcc tcggacatcc
     4021 cggagaacat cctcgacaag ccgtcgcagt cgccccagcc tcagcccacg tccgagtggg
     4081 tgccggcggg cgatcccacc cgcggccccg tgctacgcgt tgtcggcgtg ggcggcgcgg
     4141 gcgtgaacgc ggtcaaccgc atgatcgagg ccggcgtcga gggcgtggac ttcatcgccg
     4201 taaacaccga cgcgcagtcg ctccagtcgt cggaggccta cgagaccgtg cacattggcg
     4261 ccgaggtcac gcgcggtttg ggcgcgggct cgaacccgga gctcggccgg cagtcggcgc
     4321 tcgaggacta cgaccacctg aagagcctgc tgaagggcgc cgacatggtg ttcatcgccg
     4381 cgggcgcggg tggcggcact ggcaccggcg cggcgcccgt gatcgcgcgc atctcgcgtg
     4441 aggtcggtgc gctgacggtc ggcatcatca cgaagccgtt ctcgttcgag ggttcgcgcc
     4501 gcgctctgca ggccgaccag ggcgtggagg aactcgcgcg cgaggtcgac accctgatca
     4561 cgatcccgaa ctcgcgcctg ctgagcgtgc tcgacaagca gacgtcgatg gtcgaggcgt
     4621 tccgcgtggc cgacgacgtg ctgcgccagg gcgtgcaggg catctccgat ctcgtcacgc
     4681 tgccggggct gatcaacctc gacttcgcgg acgtgcgcac gatcatgtcg caggcggggc
     4741 aggcgctgct cggcatcggc atggcgaccg gcgagcaccg cgcgatcgag gcggcgcagc
     4801 gcgccgtgga gtcgccgctg ctcgagacct cggtggacgg cgcgcgctcg atcctgctgt
     4861 cgatcaccgg tggccgcgac ctctcgctgt gggaggtcaa cgaggcggcc cgcgcggtgt
     4921 ccgaggcggc gcatcccgac gcgaacatca tcttcggagc catggtcgac gagaaggtcg
     4981 tggaccaggt gtgggtcacc gtcgtcgcca ccggctacgg cgaccgcccc gtgcgaaggg
     5041 ccgctccgcg agccgagtct gcggatcgga ccgagcgctt cgagcggccc cgcagggacg
     5101 cccgcgaggc ggaccacgag ccccgcgtga cccggactcg cgtgagctct ctgggcgaac
     5161 tcgacgtccc agagttcatc ccgcgcgcgt agtctgacgg ctccaaaccg ccgctctgct
     5221 gcgtcctcgg ggattcgcgg tattggcata ccgcttcacc cctgcgtcat tgcatagctt
     5281 ggttttcgct cgtcagactc cgcgcgccgc gcctcggtag acgatcatct cgccatgaag
     5341 ggcgtcgtcg cagccggaca tccgctcacc gctcaggcgg gggcggatgt tctgcgttcc
     5401 ggcggcaacg cggtcgatgc cgcggtggct gccgtgctca tgtcgttcgt atgcgagtcg
     5461 ccactcaccg gcccgggcgc cggaggattc atgctcgtgc acacgcccga cggcgaggac
     5521 gtgctgctcg acttcttcgt cgccgctccc gggcgagggc tcgaccggct cgagccggct
     5581 ccactgatgc cgatcgacgt cgagttctcg aaggaggcgg tccaggtctt caacatcggg
     5641 ccctcgtcgt gcggcgccta cggcaccacg ctcggactgg cacacgcgct cgagcgcttc
     5701 ggcagtgtgc cgctgtcggc gctcgtggag aggccggcgc gcgcggcgcg cgagggcgtg
     5761 gagatcaacg acatccagtc gtacctccac aagattctcg ggccgatcct gtcctccacg
     5821 cccgaggcgg cggcgatcta cgagccgaag gggcgcccgt tgcgtccggg cgagacgatc
     5881 taccttcccg cgctcggcga cctgctcgag cgcctcggcg ccgagggacc cggtttcctc
     5941 tacaccggcg acgtaggcgc ggccgccagc aactggctgc tcgagcgcgg agggctgctc
     6001 acccgcgacg acctcgcggc gtacgagccg atcgagcgcg aaccggtgcg cgctcgctac
     6061 cacggccgcg acgtgctcac gaacccgccg ccctcgtcgg gcgggatcct gatcgcgcac
     6121 tcgctgaacc tgctcgagcg cctcgggcgc cgcccagatc cccgcacgct cgtcggcgtg
     6181 atggcgcgca cgaaccggga gcgcaccgag gagttcctgt cgggtctcca ctccgagggc
     6241 tatctcgaga gcttcctggc gcacgaggcg ctcgactcgg ccgcccgcga ggtgggcagc
     6301 cgactcgggt cgaccacgca catctcggtc ctcgacgacc gtggtcgctg cgcatccgtg
     6361 acgtgctcga acggctcgtc gtcaggcgtg atcgtgccgg gaaccgggct gcacctgaac
     6421 aacatgctcg gcgagcagga cctgaacccg cacgggtacc accgccacga gcccggtcgc
     6481 aggctgccga gcatgatggc gccgaccgtg gtgctgcgcg acggccgccc cgagatcggg
     6541 ctgggctcgg cgggctcgaa ccgcatccgc tccgcgatca tccaaacggc gctcgcttcg
     6601 gtgaatgacg ggctgcccgc gcaggccgcg gtggaagccc cgcggctcca cctcgaggag
     6661 gccgtcgtgg acgccgaacc cggcatcgat cctgcgggcc tcgatgccct cgaggcggac
     6721 ggctggacgg tgcggcgatg gcgcgagcgc aacctcttct tcggcggtgt gcaggctgtc
     6781 gcgcgcgacc ccgaaaccgg cgcactttcc ggcggcggcg atcccagacg cggcggcgcg
     6841 gcgatcgtcg tggcctgact actccgcatt ttccggattt tcccgggtgc ggggggttgc
     6901 ctgtcgcgga gcgagtggaa ccctcgcagg agcgaggtta acccgaatgt ggggggagag
     6961 tgcgcccgcg acttgcaccg ctggatggct cgttcctgcg cgtagaaacg ccgaacgcgc
     7021 acatgcacgt tggctggtgc gcggtcttcc ggccgcaccc cgatcggccg cgcccgagcg
     7081 tggagatgct gcgtcgcagc gtggcggggc ggttgcatct cacgccgcgt tttcgccagc
     7141 ggctcgcatt cccgccgccc ggcttcggcg agcccttctg ggtggacgcg ccggacttcg
     7201 atgcaggcag tcacataact gccctcacgt gcgacgacga cgcagtctcg ttcgagacct
     7261 tccacatgct cacggacgcc gccctgtcca cgccgctcga ccgcggccga ccgctctggc
     7321 acgtgtacct cgtgccgcgg ctcgaggacg agcgcgtggg catggtggcg aagctgcatc
     7381 acgcgatggt ggatggccta tccgcggtcg agctcggcct tctcctcctc gacaccgaac
     7441 gggacgctcc tctcccgccg ccgcccgagc cgagcgagcg ctggctgccc tcgcgggtgc
     7501 cgggacagat ctcgcttgca gtggagtcgg cggcgtcact ggcgggtgac gcgctgcgcg
     7561 gggcgcggac agccgccgcg ttcgccttct caccgcggcg tcagaccgcg gcgctcgccg
     7621 ggactctgcg gcgcgtggcc gcggtagtcc gcgacgacct tcttcccgct gcgcccgact
     7681 cggccgtgaa cgtgccgatc gggccgcagc gaactctcgt ccgcggccgc gtcgagatgg
     7741 acgctctgga gagggcgcgt acggcactcc gggccagcgg ggggccgcgc gtgaccgtga
     7801 acgacgtcta tctcgcggcc gtggcggggg ctcttcgcac gctcgcgctt cggcgcggcg
     7861 agcggccggt tccgctgaag gcgatggtgc ccgtgagcgt tcgctcggac tcggagcgcg
     7921 cctcgctcgg caaccggatc tctttcgtgt tcctcgagct gccggtgcac aagacgtacc
     7981 cagcggctcg gctagaggac gtgcacagcg cgacccgggc gttcaaggag tccggccgta
     8041 tcgccggcgg cgtgaccgtc ctgagcgcgc tcggcttcct tcccgatccg ctgaaggaca
     8101 ttgcagcgcg catggcggcg agcgcccggg tctacaacct cacgatctcg aacatccccg
     8161 gccccggcgt gccggtgttc atgctcggag ccgagctcga ggaggcgcac cccgtggtgc
     8221 cgctgtccga ggatcatgcg ctgtccgtcg gggtgttcac gtaccggagc ggggcgttct
     8281 tcgggttcta tgcggatccc gatgcgctgc ccgaggtgag tgagttgccg gccgcggtcg
     8341 cggcggaggt ggcggtgctg gccgggcagc gccctgcgcc tcgcaggctc gcggtggtct
     8401 gagcgtttac agcagcactg aggggcgggc aagcggggtg ctccgatcaa ccgtggtgca
     8461 ttttggggcc tatagaccca aaatcgacca cggtagttct cggagatggc ttcggatgga
     8521 agaagtgccc gccctcccct tactgctcca ttgaagtgcg gccgcccttc gtgagcgtca
     8581 gctccgccca gccaccggcc tcccgctccg cgaacgcgtg accctcatcc gccgcccacg
     8641 ccgccaggtc gatcggagcc tcggggtccg tggcgagcac caccagcacg tctccgtcct
     8701 ctagctcgcc catcttcccc cgggcgagcg tgagcggtag cgggcaggca aggccgcggg
     8761 cgtccacggt cgtcacaatc agtgattgtg aacgggggga cgtacgagat tgcggccgtg
     8821 ttctcgagcg ggctgcacga gcggctgtac tcggggctgt cgctggtcgt gtcgacggct
     8881 gccgagggca agcgctgcgc ggcgctagcg gcgttcggcg ggctcgcgat gttcattgac
     8941 cgcgaactga tgcagcacgc gcaggagccc gacgccacgc cgtcgctcac ctgggcgggc
     9001 cgggacacgt tcgcgcgctc gctcgccagc ctgcgcgaca ctgcgctcga gctcgacacg
     9061 ctcgacgtgt gggcgtgcgc ggcgtcggtg gagacgatgg ggctgacgga gagcgatgtg
     9121 caggaccgcg gcctgaaggg cgtcctctca acgccgcgct tcctgcgtga ggcaggcgcc
     9181 gcgacgctgc tgttcgtcta ggggccgagc gacgagggct tgatgtgccc gttcgagcgg
     9241 gcctgctcgc tcgttcgctc cacgaactcg agccggcgca tcgtggagac gaacagctcg
     9301 cccaccatgc gaagccgctc gctctccgaa cgcgcctcga gcagatcctg cttcagctcg
     9361 ggcgcgaagt cgatcgtcga cgccatcccg taggcgccga gctcctcgag gccggtctcg
     9421 tctgggcgag agtcggtgag ctgctccacc aggtccgcgt atcgctcgcg cgcctgggcc
     9481 gccagctggt cgcgctccga ctcggcggac gactcgtcgt cgagcatctc cacgtcgccg
     9541 gccgggtaag gcatgtcctc gatccgttcg actagccgga acggccgcgt gccgcgcacg
     9601 aggatgttca tgcggccgtc ctccatgcgc tcgaggagct gtgcgatctc cgcggtgcag
     9661 ccgatctcgc ggagcccgtc gggcgcgagc cagacgatcc cgaactctcg cccctcctcg
     9721 aggcactcgg tgatcatcgt cttgtagcgc tcctcaaata tgtggagcgg tacgacctct
     9781 tcgggcagca agacgatccc gagagggaag aggggcaacc gttcgacctc gtctcccacg
     9841 cgccctaggc tacgcgcatc gtgccgcggg tcgcccggcg atcggtccag tgcccggccc
     9901 gcggactcaa ccgggcgcga gaacgctgac gatacgagcc gggtggctca tccgcgcccg
     9961 ctctcgaccc ttggcgtcag gcggagaacc cttccctttc tgggcgcgct cgcggtcgcg
    10021 tttgcagtcg cgccgctgcc cgcggtagag cgagacggga gcgcgctgct tgccgcaggc
    10081 gtgctcgccg tcgccgtggt tgctgcggtc ggcctcacgc catggcaccg cctgccggcg
    10141 tggatgcagg cgctcccgcc gctcgccgtc ttccccgtga ttgccctgct gcgcgacgcc
    10201 gacggtggca cgacgtcggg ctacggcgtg ctcgtgatcc tgccggtctt ctggttcgcg
    10261 atgtacggca cccgccgcga gctgtgcatc gcgatcgccg cgatggcagc caccctgatc
    10321 gcgccgctac tgctcgccgg caacccgctt tatccctcca ccgaatggcg tcgcgtgttg
    10381 ctgtgggcgg tggcggctcc gctgatcggc ttcacggtgc agaacctcat cgcgcgcgtg
    10441 cgcggcaaga ccgaggagct cgagtcggcg gcgcgcacgg acacgctcac cggcctgccg
    10501 aaccgcaggg cgctcgacga ggagataccg gacgagctcg agcgcgccgc gcgctacggc
    10561 tacccgctct cgctcgcgat gatcgacctc gaccacttca agcgcctgaa cgacgagcgc
    10621 ggacaccagg cgggcgaccg ccttctgaag cgcgccgccg ccgcgtggcg aagcgagctg
    10681 cggcactcgg acatcgtcgt gcgctacgcc ggcgacgagt tcctcgtggc gctcccgaac
    10741 tgcactccgg cgaacgccca cgcgctgatc gaccggctcg ttcgcgcgct tccgccgggg
    10801 cagacctgct ccgcaggcgt ggcgcacctg cgctccggcg agagcgtgga ccagcttgtg
    10861 gcgcgggccg acgccgcgct gctcgccgcg aagcgcggcg ggcgcgcgca ggtcgtatcc
    10921 ggctaggcgc ccggcgcgcg cgcggagatc agcaggttgt agaagagcgc tgccggcagg
    10981 cggggctcga gcagcgcgcg gtcgaccgcc tggagcgcga ggtagcctcg gtacgcgtac
    11041 tggcgccacg cccagggaac cgagtccggg tcggcggtcg actcgagcgt gcgattcgcc
    11101 cagccgaaga ggctcgccgc gagctcctcg ccgcgcacac gcacctgatc gaaccccgca
    11161 tggcgcgcga agccgtacag gtccgcgggc gtgaaggcgt gcacgtcgac gaccatctcg
    11221 agccggtgct cctcctcatc agccgggtcg gccacgtgcc cgttcgggcg gatgccggcg
    11281 cgcatcagcc gccgccacgc gggggcggcg gcgagtgcgg cacgcttcgg cacgctcgct
    11341 atgcggtcgc cgtagtacga gggctcgccg cagaacgcca ccatgccgcc gggccgcagc
    11401 acacggcgga actcgcggaa cgcagcgtcg aggtcgggca ggtgatgcag caccgcgtgt
    11461 ccgaacacga ggtcgaagga cgcgtccggg aacggcagct tcgccgcctc acagcgcgcg
    11521 gtctcgacct cgagcccgag ccgctcggct gactccgaca gtgcgtcgag catgccctgc
    11581 gagatgtcgg tggccaccga ctcgccgatc acgcccgcgc gcagcaggtt gagtgagaag
    11641 tagcccgtgc ccgcgccgat ctcgagcgcc cgcccgaaat gcggaagcgg agcgccgatc
    11701 gccttctgca gcttcgcggt gacctgacgg cggccgggct cgtcgtagtt gatgccccac
    11761 ttcgtgtcgt aggtctcggc cgccacgtcg tggtagcgga cgttcacgtc tttgatgcgc
    11821 tccgccgctt ctgccttcat gcgggcgggc acgatacagg caccggggcg ctatgctcgc
    11881 cgccgttttg gcccactccg ctgcggaata cgtacagcgc gaccagccgg acggcgtgcc
    11941 gccgctcgag ctcacggggg agcgaacgct tcccgatgtg cccgaggaga actactggtt
    12001 ccgccgccat ctcgcagtgt acgagtggat cgccgcgcgc tgtgaggggc tcgatgtcat
    12061 cgacatggcg tgcggcgagg gctacggcac tgacgtgctc gcgcggcgcg ccaagaccgt
    12121 gaccggagtg gacgcgaacc ccgaggcgca cgagcacgcg tgcctgaagt acgcgcgccc
    12181 cggcgtgaag ttcgtgcgcg acctcgtgga gacctacagc gagccgtgcg acgccgtcgt
    12241 gttcctccag acgatcga
//
LOCUS       DASVXS010000108         6004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_454387, whole genome shotgun sequence.
ACCESSION   DASVXS010000108 DASVXS010000000
VERSION     DASVXS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454387"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VF752_06085"
     CDS             complement(<1..637)
                     /locus_tag="VF752_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX8855154.1"
                     /translation="MRTQVELPNNVASELAGPGDAVLRSLEGHLEAEVFLRGNVLTLE
                     GEERDVEMAQTVVRELSDLIRQGHEIAPGTISAITSALDRHESPSRILEDVVWRHRGT
                     KVAPKTVNQKRYVDAIRNNTVTVGIGPAGTGKSFLAVAMAVAALSRREVNRIVLTRPA
                     VEAGEWLGFLPGDLMAKVDPYLRPLFDALYDMLEPERVNQHLERGVIEVAPL"
     gene            complement(637..1071)
                     /locus_tag="VF752_06090"
     CDS             complement(637..1071)
                     /locus_tag="VF752_06090"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX8855155.1"
                     /translation="MALIDRIKQDTTTAMKAGEKERVQALRLLTNELQKALKDGNGAD
                     EAAVLQRERKRRLEAADAYGEAGRTDLAEAEKREAELIAGYLPEQLSDEELHAIVGDA
                     IAESGASSAREMGQVMSMVMPRIQGRADGRRVSQLVKEKLTA"
     gene            complement(1382..2452)
                     /locus_tag="VF752_06095"
     CDS             complement(1382..2452)
                     /locus_tag="VF752_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8855156.1"
                     /translation="MSTAVALLVLVGPARAAVTTVHPGESIQAAIDAASPGDKIVVEA
                     GVYHENLTIAKNGITLRGAGARESGTVLKPAEPPTPTPCEGPPTGICVRGEMDPTTGE
                     PGTPVDGVTIKGFLVDGFPAHGVGSINATDFTVKRTEARNNGGYGIVGFVQSGIRYLD
                     DVAHDNGEPGFYIGDSPQANAEVVGNRSFHNVGPFEGFGFLFRDASHGLVKDNRAWDN
                     CLGYFLVDSGENPDPVSDWTLKGNEANGNNQGCTGGGEEQLPPFSGTGILLAGTNATV
                     VKHNRVFDNVPSLDSPFSGGIVLVDGTPLGGAVPSDNTIKRNTALGNEPADLIWDGTG
                     QGNVFDANRCDVSNPEGLCAGG"
     gene            2580..3284
                     /locus_tag="VF752_06100"
     CDS             2580..3284
                     /locus_tag="VF752_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX8855157.1"
                     /translation="MARDFDWCTFDCYGTLIDWEGGAAGFLYEMALREGDNRARSGRE
                     LRDHWEAIQFELVTGPYKPYKQILAESLEEFAQAGGWYYDSGDGEALVTSMRSWQPFP
                     DTTPALTRTREAGVKLAILSNTDRDIIEHSLKHLEVPFDEVVTAEDVGSYKPSDANFE
                     RVLDVVGAPAERVLHVAFGFKYDIGPAQRAGMKTAWVNRHVETAPGPERPDHEWRDLW
                     GLAQLATGEAPPVPSA"
     gene            3360..5606
                     /locus_tag="VF752_06105"
     CDS             3360..5606
                     /locus_tag="VF752_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8855158.1"
                     /translation="MTQTDSQMNQSARAETRPGLAARAGGWSARHRKEAIFGWLGFVI
                     VALVLGSAIGTKHMADADGSSGETHRAEQMIDRNFPPIAGESVLIQSSTHKPGSSQFQ
                     AAVKDVGRRLDAIPDVKRLKLPTRANGGYSKDGRSAIVTFDVPDPASKEAKKRVDPML
                     NAVAAAEKSHPGFRIDEFGDASANKALDDTLGKDFERAEKLSIPMTLVILIFAFGALV
                     AAGLPVLLAISAVAATMGLVAIPSQVFPVDSGIASVILLIGMAVGVDYSLFYLRRERE
                     ERARGRDPRSALAAAAATSGRAVLVSGLTVMVAMAGMFITGSKTFSSFGVGTILVVFV
                     SMIGSLTVLPAMLSWLGDRVDKGRIPFVRRLRRADGEPRVWGRVVDGVLRRPVVSVLL
                     SGAALVAIALPALGMNTKLTGFEDLPKKLAIVQTYDRLNQAFPGGQVPANVVVEAKDG
                     VRQPQVQAAVAQLEQKALASGEMFGPIDTNVSPNGKAEKIYIPLAGAGTDAKSNHALA
                     TLRNDLIPRTVGSVPGVNAATTGETAGTKDFNDLMAARTPLVFAFVLGLAFVLMLVTF
                     RSIVIPLKAIALNMLSVGAAYGLLVWVFQDGHGEKLLGFHSNGAITAWLPIFLFVVLF
                     GLSMDYHVFIISRIKEAVDRGMKTEDAVAHGIKATAGTVTSAAFVMVAVFAIFATLTT
                     IEMKELGVGLAAAILIDATIVRAVLLPATMKLLGKWNWYLPKPLRWLPRTNHAPVLPD
                     RRPEPAKA"
     gene            complement(5596..>6004)
                     /locus_tag="VF752_06110"
     CDS             complement(5596..>6004)
                     /locus_tag="VF752_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855159.1"
                     /translation="RPNQRREQRAVQEVTQLAKGADRAPLLSGRTYRNDFYDTLRLGR
                     FKGSFPLEFGGTDPDDGRFYDGDVGFSGGRVTFRVVIPKTRISVHYKVRTCRQLTATL
                     QVDVPPSNAVTAMDATATKAAASNRQGRGEVRP"
BASE COUNT          885 a         2096 c         2035 g          988 t
ORIGIN      
        1 cgagcggcgc gacctcgatc acgccgcgct cgaggtgctg gttcacacgc tccggctcga
       61 gcatgtcgta gagggcgtcg aacagcgggc gcaggtacgg atcgaccttc gccatcaggt
      121 cgccgggcag gaagccgagc cactcgccgg cctccacggc ggggcgcgtg agcacgatcc
      181 ggttcacctc gcggcggctg agcgcggcga ccgccatcgc cacggcgagg aacgacttgc
      241 cggtgccggc cggcccgatg ccgacggtga ccgtgttgtt gcggatcgcg tcgacgtagc
      301 gcttctggtt gaccgtcttc ggcgcgacct tcgtgccgcg gtggcgccag acgacgtcct
      361 cgaggatgcg gctcgggctc tcgtgccgat cgagcgcgct cgtgatcgcg gagatcgtgc
      421 cgggcgcgat ctcgtgtccc tggcggatca ggtccgacag ctcgcgcacg accgtctgcg
      481 ccatctccac gtcgcgctcc tcgccctcga gcgtgagcac gttcccgcgg aggaagacct
      541 cggcctcgag gtggccttcg agggagcgca gcaccgcgtc acccgggccg gcgagctcgc
      601 tcgccacgtt gttcggaagc tcgacctggg ttctcactag gccgtgagct tctccttcac
      661 gagctggctc acccggcgcc cgtcggcgcg cccctgaatc cgcggcatga ccatcgacat
      721 gacctgtccc atctcgcggg cggagctcgc gcccgactcg gcgatggcgt cgccaacgat
      781 tgcgtgcagc tcctcgtcgg agagctgctc gggcaggtat ccggcgatga gctcggcctc
      841 gcgcttctcg gcctcggcga gatcggtgcg gcccgcctcg ccgtaggcgt cagcagcctc
      901 gaggcggcgc ttgcgctcgc gctggagcac ggcggcctcg tcggcgccat tgccgtcctt
      961 gagggccttc tgaagctcgt tcgtgagcag gcgtagagcc tgaacgcgct ccttctcccc
     1021 cgccttcatg gcagtggtcg tgtcttgctt gatgcggtcg ataagggcca cacatcaagt
     1081 gtaggccggg gtcccggcgg cgggtggcgg tcgccgcggt gcccggaaca gcagcaataa
     1141 cgggttggac tcggttcgct ctgggcctcc ggcgaatgac atgcataact gcggatgagt
     1201 ggcccgggtg cggcaggcgt ctgcagttat tcatgacatg catgcataag tgcggatgac
     1261 cggcgcaggt gcggcaggcg tctgcagtta tgcatgtccc ggtccaggcc gcctctcatt
     1321 gctgcgtttt ggctacgggg agagtcccgg cgccggggtc ccggcggcgg gtgtcgctcg
     1381 ctcagccccc ggcgcagagc ccctcgggat tcgacacgtc gcagcggttc gcgtcgaaca
     1441 cgttgccctg tccagtcccg tcccagatca ggtccgccgg ctcattgccc agggcggtgt
     1501 tccgcttgat cgtgttgtcg ctcggaaccg ctcctcccag cggcgtaccg tccacgagca
     1561 cgatgccgcc cgagaacggc gaatcgagcg aggggacgtt gtcgaacacc ctgttgtgct
     1621 tcacgacggt cgcgttcgtc ccggccagca ggatgcccgt tcctgagaac ggcggcagct
     1681 gctcctcgcc tccgccggtg catccctggt tgttgccgtt tgcctcattg cccttcagcg
     1741 tccagtcact cacagggtcg gggttctcgc ccgagtcgac gaggaagtag ccgaggcagt
     1801 tgtcccacgc gcggttgtcc ttcacgagcc cgtggctggc gtcgcggaac aggaagccga
     1861 acccctcgaa cggtccgacg ttgtggaacg accggttgcc gacgacttcg gcgttcgcct
     1921 gaggcgagtc gccgatgtag aagcccggct cgccgttgtc gtgggcgacg tcgtccaggt
     1981 agcggatccc cgactggacg aacccgacga tcccgtagcc gccgttattg cgagcttccg
     2041 tccgcttgac ggtgaagtcg gtcgcgttga tcgagccgac gccgtgggcc gggaagccgt
     2101 cgacgaggaa gcccttgatc gtcacgccgt cgaccggtgt gccgggctcg ccggtcgtcg
     2161 gatccatctc cccgcggacg cagatgccag tcggcgggcc ctcgcacgga gtcggcgtcg
     2221 gcggctcggc gggcttgagg accgtcccgc tctcacgtgc accggcgcca cgaagcgtga
     2281 tgccgttctt cgcgatcgtg aggttctcgt ggtagacacc cgcctccacg acgatcttgt
     2341 cgcccgggct cgccgcgtct atcgcagctt gaatcgactc gcccggatgg acggtggtga
     2401 ccgccgcgcg cgcgggcccc acaaggacaa gtaacgccac cgccgtgctc accaaggtca
     2461 cgaatgtcct gagccgtagg cgcatgaggc ctcctacatc cggggtggcg tcggcaagac
     2521 cgtaggcggc ggtatagagc gggtcaagca ggctggcgcg ccgatactct gcagcgccag
     2581 tggcccgcga cttcgactgg tgcaccttcg actgctacgg caccctcatc gactgggagg
     2641 gcggcgccgc cggcttcctg tacgagatgg cgcttcgcga gggcgacaac cgtgcccgca
     2701 gtggccgcga gctgcgcgac cactgggagg cgatccagtt cgagctcgta accggtccgt
     2761 acaagccgta caagcagatc cttgccgaga gcctcgagga gttcgcacag gcgggcggct
     2821 ggtactacga cagcggcgac ggcgaggcgc tcgtcacgtc gatgcgcagc tggcagccgt
     2881 tccctgacac cacgccggcg ctcacccgca cccgtgaggc cggcgtgaag ctcgcgatcc
     2941 tctcgaacac cgaccgcgac atcatcgagc actcgctcaa gcacctcgag gtgccgttcg
     3001 acgaggtcgt gaccgccgag gacgtgggct cgtacaagcc gagcgacgcc aatttcgagc
     3061 gggtgctcga cgtggtcggg gcgccagcag agcgcgtcct tcacgtcgcc ttcggcttca
     3121 agtacgacat cggcccagcc cagcgcgccg gtatgaagac cgcctgggtg aaccgccacg
     3181 tggagaccgc gcccggcccc gagcggccgg accatgagtg gcgcgatctc tggggtctgg
     3241 ctcaactcgc cacgggcgag gctccaccgg tccccagcgc ctgacttcgg gctagcccca
     3301 aggccaagac gcctgccccc ctcattcccg cgcgggcccg cgcggacgac actcgtcgca
     3361 tgactcaaac ggactcgcaa atgaaccaga gcgcgcgcgc tgagacgcgc cccgggctcg
     3421 ccgcccgcgc ggggggctgg agcgcccgcc accgcaagga ggccatcttc ggctggctcg
     3481 gattcgtgat cgtcgccctc gtgctcggct cggcgatcgg caccaagcac atggccgacg
     3541 ccgacggctc gagcggcgag acgcaccgcg cggagcagat gatcgaccgc aacttcccgc
     3601 cgatcgccgg cgagagcgtg ctcatccaga gctccaccca caagcctggc agctcgcagt
     3661 tccaggcggc ggtgaaggac gtcggcaggc gcctcgacgc catcccggac gtgaagcggc
     3721 tgaagctccc gacgcgcgcg aacggtggct actcgaagga cggccgctcc gcgatcgtga
     3781 ccttcgacgt acccgacccc gcctccaagg aggcgaagaa gcgcgtcgac ccgatgctga
     3841 acgccgtcgc ggcggccgag aagtcgcacc ccggcttccg catcgatgag tttggggacg
     3901 ccagcgccaa caaggcgctc gacgacacgc tcggcaagga cttcgagcgc gcggagaagc
     3961 tctcgatccc gatgacgctc gtgatcctga tcttcgcctt cggcgcactc gtcgccgcgg
     4021 gtctgcccgt gctgctcgcg atctcggccg tggccgccac gatggggctc gtcgcgatcc
     4081 cgagccaggt cttcccggtg gacagcggca tcgcctcggt gatcctgctg atcggcatgg
     4141 cagtgggcgt ggactactcg ctcttctacc tgcgccgtga acgcgaggag cgggcgcgcg
     4201 ggcgcgaccc gcgctcagcg ctggcggccg ccgccgccac gtccggccgc gcggtgctgg
     4261 tctccggcct caccgtgatg gtcgcgatgg ccggcatgtt catcaccggc agcaagacct
     4321 tcagctcgtt tggcgtcggc acgatcctcg tggtcttcgt gtcgatgatc ggctcgctca
     4381 cggtcctgcc cgcgatgctc tcgtggctgg gcgaccgcgt ggacaagggc cgcatcccgt
     4441 tcgtgcggcg gctacgccgc gccgacggcg agccgcgcgt gtggggccgc gtggtcgacg
     4501 gcgtgcttcg ccgcccggtc gtgtcggtgc tgctctcggg cgccgcgctc gtggcgatcg
     4561 cgctgcccgc tctcggaatg aacaccaagc tcacgggctt tgaggacctg ccgaagaagc
     4621 tcgcgatcgt gcagacgtac gaccgcctga accaggcgtt ccccggcggc caggtcccgg
     4681 ccaacgtggt ggtcgaggcg aaggacggcg tgaggcagcc ccaggtgcag gccgccgtcg
     4741 cgcagctcga gcagaaggcg ctcgcgagcg gcgagatgtt cggccccatc gacacgaacg
     4801 tctcgccgaa cgggaaggcg gagaagatct acattccgct cgccggcgcc ggcaccgacg
     4861 cgaagtcgaa ccacgcgctc gcgacgctgc gcaacgacct gatcccgcgc acggtgggct
     4921 cagtgccggg cgtgaacgcc gctaccaccg gcgagaccgc gggcacgaag gacttcaacg
     4981 acctcatggc ggcgcgcacg ccgctcgtgt tcgcgttcgt gctcggcctg gcgttcgtgc
     5041 tgatgctggt cacgttccgc tcgatcgtga tcccgctcaa ggcgatcgcg ctgaacatgc
     5101 tgtcggtggg cgcggcttac ggcctgctcg tatgggtgtt ccaggacggc cacggcgaga
     5161 agctgctcgg cttccactcg aacggcgcca tcacggcctg gctcccgatc ttcctgttcg
     5221 tggtcctgtt cggcctctcg atggactacc acgtgttcat catcagccgc atcaaggagg
     5281 ccgtcgaccg cggtatgaag accgaagacg cggtggcgca cggcatcaag gccaccgccg
     5341 gcacggtcac gagcgccgcc ttcgtgatgg tcgccgtgtt cgcgatcttc gcgacgctca
     5401 ccacgatcga gatgaaggag ctcggcgtgg gcctggcggc ggcgatcctc atcgacgcca
     5461 cgatcgttcg cgcggtgctg ctgcccgcca caatgaagct gctcggcaag tggaactggt
     5521 acttgccgaa gccgctccgc tggctgcccc gcacgaacca cgcgccggtg ctccccgacc
     5581 ggcgcccgga accggctaag gcctgacctc cccccggcct tgccgattgg acgcggccgc
     5641 cttcgtggcg gtcgcgtcca ttgccgtcac cgcattgctc ggcggcacat ccacctggag
     5701 ggtcgcggtg agctgccggc aggtccggac cttgtagtgg acggagatcc gcgtcttcgg
     5761 gatgaccacc cggaacgtga ccctgccgcc actgaacccc acgtcgccgt cgtagaacct
     5821 cccgtcgtcc gggtcggtac cgccgaactc gagcgggaac gagcccttga atcgcccgag
     5881 gcgcaaggtg tcgtagaagt cgttgcggta ggtccggccc gagagcagcg gtgctcggtc
     5941 tgcacctttc gcgagctgcg tcacctcttg gacagcacgc tgctcgcgcc tctgattcgg
     6001 ccgg
//
LOCUS       DASVXS010000109        21915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1374111, whole genome shotgun sequence.
ACCESSION   DASVXS010000109 DASVXS010000000
VERSION     DASVXS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21915
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374111"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VF752_06115"
     CDS             <1..821
                     /locus_tag="VF752_06115"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8855160.1"
                     /translation="LIERLREERPDVAFVALHGRDGEDGTVQELLEILGIPYTGSGVL
                     ACMRCMDKVTAKHLMLEAGIPTPDFFAFNETAFRSLGAADALPAIEERLQFPIVVKPA
                     AQGSALGIKFARTPNDVPGALVAAFSYDQRVMLERHVAGRELAVSILEGAALPIVEAV
                     PTADDFYDFEARYEIGRTEFICPAQLPETVAARAEALAVSTYKLLGCFGFARVDLMLE
                     EDTGDLYVLEANAIPGLTETSLLPQAAEAAGIAFDALVERILELAFERAAGAPV"
     gene            832..1722
                     /locus_tag="VF752_06120"
     CDS             832..1722
                     /locus_tag="VF752_06120"
                     /inference="COORDINATES: protein motif:HMM:NF004526.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8855161.1"
                     /translation="MAINGQRILITGAARGIGAGSAKALAKRGARVSLVGLEPEELEK
                     VAAECGPDAIWFEADITDQDALDAAVEGTVEKFGGLDVVISNAGIGAGGPLQLMEQDM
                     WEKVVEINLIGNVRTIRTCLPHIVESKGYVLPIASAAAIVHGPLMTAYCAAKAGLEAV
                     GNGLRVEMKPHGVDVGVGYFSWIDTDMVSGGDEHPFFRKMRGELPGPMGKTYPLSDAV
                     AAVVDGVEHRRRWVMAPKWLRAMFFLRGAVAAIDNKRIIKMMPEAEHAFREEIEAKGA
                     DASAPTGAGGAAAVRAHSAT"
     gene            1742..2293
                     /locus_tag="VF752_06125"
     CDS             1742..2293
                     /locus_tag="VF752_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HEX8855162.1"
                     /translation="MRSLLVFVPVLGAPILHAPVLALDVLPGLKRPLDFGASWRGRRI
                     FGGNKTWRGALFMVAGVLIAALALWQWPAYRHALPDDIENASPALIGVVIGLGVVLGE
                     LPNSFAKRRLDIGPGRRASGLWGVALSVWDQADFVPLVALLLLPIWQIPFEDLLLAIV
                     VVGGVHFGINLIGYAIGARKTLV"
     gene            2327..2686
                     /locus_tag="VF752_06130"
                     /pseudo
     CDS             2327..2686
                     /locus_tag="VF752_06130"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            2775..3176
                     /locus_tag="VF752_06135"
     CDS             2775..3176
                     /locus_tag="VF752_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855163.1"
                     /translation="MSERTNVEIVRRVYEAWARDELPGPARLFDAEIEYVNPPNAVEP
                     GTRHGLAAFSRAVHDAFEGWETWEIEPERFIPVGERVAVVVRYRAHWQTSGVDVVAHE
                     SALWTVRGGKVVRYEWFHGPHDALEAASLRE"
     gene            complement(3330..3812)
                     /locus_tag="VF752_06140"
     CDS             complement(3330..3812)
                     /locus_tag="VF752_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX8855164.1"
                     /translation="MVIYGVSFDMVGKQPIVLLKTRDGNKFLPIWIGHPEAAAILMKL
                     QGANTPRPMTHDLISDMLSEMEVACTRVAVTELKENTFYASITLRVDGRDVEIDSRPS
                     DALALAVRTSAPIFAAEEVIAESAIEFEHEVEDTEEVVEKFKDFLDNVTPEDFMGGEQ
                     "
     gene            3902..4786
                     /locus_tag="VF752_06145"
     CDS             3902..4786
                     /locus_tag="VF752_06145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8855165.1"
                     /translation="MTLPTRIVVCTNRRPDQVAECLTALRAQAGDEAPLLVTSGLRPE
                     QVEGHREAFPGTVIEEPRPGLSLARNRALDACPNDGVIAFVDDDAVVEPGWWEALKRR
                     WEEAPDEVAVIGGPIRPRYDTPPPRWLSRPLLPALTLLDLGGRVLDLDPEVTTVYGAN
                     ISFRVAPLREVGGFDPDFGHSGKRVFFSEEDEAQRELARVGHTVRYVPDAAVLHVIPP
                     ERLTRAAFVRRRFWYGVTLGARQARPTWLALRQMVSSAAGAPVAALRRNDRLFMERLV
                     RAAENAGVLAARARSGRA"
     gene            complement(4761..5396)
                     /locus_tag="VF752_06150"
     CDS             complement(4761..5396)
                     /locus_tag="VF752_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855166.1"
                     /translation="MLHILVADDHGIVRSGLRMLIERQDGMSVVAEAEDGVEALSLAQ
                     SVRPDVAVLDVSMPKMTGLQAAREIGAHCPGTSILLLSMHDDERYVFEALKVGAAGYV
                     LKRAADTDLIDAIRAVARGEQFLSPSSESVLVKEWLADGAEQPRDLLTPRELDVVKLI
                     AEAHTNKQIAEILHVSEKTVESHRANVLAKLGMRDRVELVRYAIRRGLIEP"
     gene            complement(5396..6361)
                     /locus_tag="VF752_06155"
     CDS             complement(5396..6361)
                     /locus_tag="VF752_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8855167.1"
                     /translation="MNKRESLIGQILAVNVFLVTATIFAASMASRLNLAVRDERVQFT
                     LLALAIVLALSANIMMLRRRFNPLERLIEQLETVDPAQPASFDPPPGGTDEVERLVAS
                     FQRLLDRIEGERRRSGRLVLRAQEEERKRLARDLHDEVNQALTAILLRLEALAQGSSS
                     PEQEEEVAELKRLVNQAMDELLQLARQLRPTALDDHGLVPALESQVRRFGEQTGIRAR
                     LQTRGEPDILGDDQQLVVYRVAQEALSNVARHARASQVHVDLSAVNGGVDLCVRDDGG
                     GFDAQRPDGGGLGLNGMAERARLVGGDLSIYSSPGQGTTVTLHVP"
     gene            complement(6519..7577)
                     /locus_tag="VF752_06160"
     CDS             complement(6519..7577)
                     /locus_tag="VF752_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8855168.1"
                     /translation="MRAYVNRSVRQADVPPPSGDAAAFHAALEGYQPTPIHELRDVAA
                     ELGLRAVSLKDESDRLGLPAFKVLGASWAVERAFREQQGVRTLVAASAGNHGRAVANV
                     AARRGLNCRVYLPARASKARRDGIAAEGADVVVVEGTYEEAVAQAAAEGVQDGVLELA
                     DVGASGPASWVIDGYATLFAEVVAAGEPFDLILVPAGVGSLAAAAARFAAQAGAKVIG
                     VEPVTAACLSASLIAGEPTEVPTPGTTMAGLDCAEVSVAAWPSLRAGVHGTITIRDAE
                     AHAAMRELAALGLAIGDCGAAPLAALRALAAEPEAAALRDAVGLGPDARVLLVATEGP
                     TDPDAYRRVVEPALDQSA"
     gene            complement(7574..8749)
                     /locus_tag="VF752_06165"
     CDS             complement(7574..8749)
                     /locus_tag="VF752_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8855169.1"
                     /translation="MSDERLPGTLEASLLRDDVVALTTQLVAIDSVNPSLVPGGAGEA
                     QIATFVAGWARQAGLEAELTTGTPARPSVIVRAPGTGGGASLLLCAHMDTVGVEGMTD
                     PHAPRVDGDRLYGRGAYDMKAGLAAALVACRELAREGVAGDVVVAAVADEEHASLGVQ
                     EVLSSIGADAAIVTEPTELEVVVAHKGFVWAEFEVTGRAAHGSRPHLGVDAIVKTGPI
                     LTAIGDLDAALGARTHPLLGRGSVHASLIEGGVEMSSYPARCVLGLERRTLPGDTAGA
                     VEDELASLLDSCRAADPELVATQRTVLVREPFEVDQDADVVEVVREAAAAVLPSAPAI
                     GGASYWADAAFIAAAGIPTVMFGPGGEGAHAAEEWVSISDTVAVTRTLIAAGTRLCA"
     gene            complement(8759..10126)
                     /gene="hemA"
                     /locus_tag="VF752_06170"
     CDS             complement(8759..10126)
                     /gene="hemA"
                     /locus_tag="VF752_06170"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX8855170.1"
                     /translation="MQGLLCLGVSHKTAPVAVRERLALPEGRAVSVLVELVADPAIEE
                     AVSISTCNRTELYVVASDPVEAETVVLSALARQAELRPTELLGHLYSLRSTEAVEHLF
                     SVAAGLESMIIGEAEIQGQVKRAYELALVEGVTGPITNRLFRDALAAGKRVRTETGVG
                     RSRVSVSSVAVDLARGVMGELERRRVLVIGAGENGELTAQALRERGVSTVFVANRRYD
                     RAIGLAQRFGGVAVRFDDLPVELMEADIVVSSTGSPHTIVGRDELELVMDTRAGRPLL
                     LIDIAVPRDIDPRVRDLSGVTLYDIDDLQREVARNLSGREAEATRARALVQQDGARFA
                     EWMATLDVVPTIAALRARGDDIVEHVLQENSPHWESLSEADFERIEAMARSIVSRLLH
                     EPTLRMKRAAEDGRFYAQLQALRELFALGDDGESPAAEPRREHRERPPARVTSLDERR
                     RSRKP"
     gene            complement(10126..10290)
                     /locus_tag="VF752_06175"
     CDS             complement(10126..10290)
                     /locus_tag="VF752_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855171.1"
                     /translation="MPALPLDEAGKYVAAAYIVFLTLVVLYVAIMAMKVSRIEREVES
                     LHELLDERGK"
     gene            complement(10292..11050)
                     /locus_tag="VF752_06180"
     CDS             complement(10292..11050)
                     /locus_tag="VF752_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEX8855172.1"
                     /translation="MYSKGLRPLAAASVVFMAASLSLVFFYAPLDADQGFIQKIFYLH
                     VPLAIVALLGFIAGGVMAIKYLRTRDPSWDMRSYVAIHLSLVLAVGVLITGSIWAKGS
                     WGHWWVWDEPTLVSFLIIFLLYCCYQPLRFSIEDPERQARYAAVFAITAGAFVPVNFI
                     AVRLAQAFSHPRVLSTTGGNMPGEMRLTFYVSLIAMGLLFVTLWKFELVSKHAKFELK
                     RLRRRLELSLEEGTSRGGAPSPPTATVPAQPIGS"
     gene            complement(11091..11765)
                     /locus_tag="VF752_06185"
     CDS             complement(11091..11765)
                     /locus_tag="VF752_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX8855173.1"
                     /translation="MTRAARAILRKDLAIEVRTRESVFPMVLFSITVFVLFHFGLGRN
                     ELDGDLASGVLWVTLLLATILAVNRLFAAEHEQGGLEGILLAPIDRTAVFLAKSTALL
                     LYLVVLEVVALPAFALLLLGPNLLDAFPELLAVLVLADVGLAVVGALVSGLASETRAR
                     DLIVPLLLLPLLVPVVIGAANATEPLMSLSPHPEDLGKWLGLLALYDGVFALVAYAVY
                     DFLLED"
     gene            complement(11762..12436)
                     /locus_tag="VF752_06190"
     CDS             complement(11762..12436)
                     /locus_tag="VF752_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855174.1"
                     /translation="MNDTAVEAVGLERRYGERVALTDVSFELPAGATLGVFGQNGAGK
                     STLLRVLATLLRPHAGRVRVLGNEVPQESWRVRGQVGLLGHDPLLYRELSGRENLLFH
                     ARLYGVARERVEEVLASVGMERRAGDPVRELSRGMVQRLAAARAVLHDPPLLLLDEPR
                     AGLDPGAAELLEPLIGRASGRTRVLVTHDVEHGLAESDTAIGLRAGRQVIAGRLTVEQ
                     VRELYR"
     gene            complement(12429..12893)
                     /locus_tag="VF752_06195"
     CDS             complement(12429..12893)
                     /locus_tag="VF752_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX8855175.1"
                     /translation="MRAAVVTISTTLARGEGDDVSGPALVDLCNEAGLETVHEVVSDD
                     RDAIASALKRLADVERVRFIFTTGGTGLTLDDVTPEATRDAIDREAPGYAEAIRAESR
                     NHTPLGILTRGISGVRGRTLIINFPGSPKGVAQSWPLVEPTLRHAAETLERE"
     gene            complement(12903..13394)
                     /gene="moaC"
                     /locus_tag="VF752_06200"
     CDS             complement(12903..13394)
                     /gene="moaC"
                     /locus_tag="VF752_06200"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX8855176.1"
                     /translation="MSADPDRLSHLDERGRARMVDVGGKQVSDRRAVAEAVVRMSPET
                     AAAVAAGDAPKGDVVSTARIAGIQGAKRTAELVPLCHPLPLSFVDVGIEVDPGTGEVR
                     VTAEARTSAQTGVEMEAMTACSVAALTVYDMVKGLERGVEIGPVRLLEKSGGRHDWRR
                     DPA"
     gene            complement(13405..14163)
                     /locus_tag="VF752_06205"
     CDS             complement(13405..14163)
                     /locus_tag="VF752_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX8855177.1"
                     /translation="MPAQAYSGKECVCQLRRGICDQSCVQDMLDTPFYIACLRLNGRR
                     CVVVGGGEIGLEKVEGLLACDASVVLISPHAIEPLQELDAEGSIAWERREYRREDLEG
                     TFMVIAATSDTNVNIRVYEDAEERAMLVNVVDVPPLCNFILPAIFRSGPLAIAISTAG
                     ASPALAKRIKSEIAEEYGEPYARLAELLNEVRGWAKGTLPTYQDRKVFFESIVNGEPD
                     PVELVRQGDEQAVRDIIAAAQRTAGEELAAGRAA"
     gene            14332..15591
                     /locus_tag="VF752_06210"
     CDS             14332..15591
                     /locus_tag="VF752_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8855178.1"
                     /translation="MPAAASAVTVGQNGNNFACGGAGYSEMQVSVAGPPDYVVPSGGG
                     VITSFRVLGNADTGDTVKLQIFRPAGSGNYTVTGESAVQGPLTPNTLNGFNTRVPVRA
                     GDILGLAIVTTNGSPPCVFSTSSSGDVIGQYNVTPAVGTTGPPSASVGNLRVNVSAEV
                     EADADDDGFGDATQDQCPTDPSTQGPCPGPTVSGTAVNGQALTATRGGNPNNLSFQWL
                     RCDASGNGCAPIPGATAASYTLTFFDIGHAIRVRQLQSNASGSRTIDSAATAAVAPAP
                     GRCTNARNGTAGNDTLLGSTGGDLIHGLAGRDSINGANGGDCLYGDAGNDRVTGAAGG
                     DRVYGATGRDRLSGGAGNDRLFGGAGSDRISAGAGKNRVSGGSGNDVINTANGQRDTV
                     NCGTGTRDRIRADRIDRLRGCEIRRLV"
     gene            complement(15597..17180)
                     /locus_tag="VF752_06215"
     CDS             complement(15597..17180)
                     /locus_tag="VF752_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8855179.1"
                     /translation="MESATAAAPAELESFNPATGERLGAVPVIAPEAVQSVVDDVAEL
                     QPFWAQLPTLDRAAYMRRAAQAIVDEVEELARLLSREQGKPISESYSMELLPTIDGLH
                     WIADNGPDLLADEDVPMRQIFVRQKSAWLSYEPLGVVGVFAPWNYPWSIPFGEVAMAL
                     MCGNGVVLKPAPLTPLIGQRIQSVFERAGVPEGLVRCVHGGGAVGQALVESSVAKVFF
                     TGSVETGRSVAASCAEQVKGCVLELGGKDPQIVLADAQLPNAVSGCLWAGFANAGQTC
                     SGIERVYVAREVAAEFIDDVVAGAKALRVGDPLDPETEIGPMVSEAQFDLVRELVDDA
                     VASGAELLCGGPLDVSSMPGRFFAPAVLTGVTPEMRIMREEIFGPVVPIVTVDSEEEA
                     IQLANDSRFGLGASVWTCDRDKGARISRRLEAGMVWLNDHMFSHGLMQCAWGGVKESG
                     VGRAHSKFGFYECVNVKQVTWDPARARNFWWHPYDSSMPKALDAAATLLYGRDTQKLG
                     ALRRGAIPLARLAARMLRR"
     gene            complement(17202..17753)
                     /locus_tag="VF752_06220"
     CDS             complement(17202..17753)
                     /locus_tag="VF752_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8855180.1"
                     /translation="MASPQTARQLVDPGAMLGIVHELDSGLRLGVRLARASDAPRVRA
                     FLERLSPETLHRRFFTSVPEVQDRLVEHFTFYDPRERLVLAATAPVEGVEEIVGLADI
                     ALLETGLAELGLVVDDEHQRQGVGTLLAEAIASLAIQRGATRLKAEMLDGNRGMLQVM
                     EQLGPTMQTVERGNRVVYTRLPE"
     gene            complement(17899..19224)
                     /locus_tag="VF752_06225"
     CDS             complement(17899..19224)
                     /locus_tag="VF752_06225"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX8855181.1"
                     /translation="MEKLFETDEAPRGRTAVPPEHLPLAVRMRPISLGEFVGQEHLLG
                     EGSSLRAAIEEGRPHSMILYGPPGTGKTTLARLLAKNARAAFEELSAVNAGRAEVRDV
                     IERAEHRRKTTGEGTIFFLDEIHRFNKAQQDALLPAVEEELVVLVGATTENPYFEVNS
                     ALLSRSTVYELRTLEEPHVLALLRRSLSDERGIPDAPPVDDGALEFLAARSGGDARTA
                     LAALELAVETAAGEAVTIEVAEDAMQRKALLYDKGGDRHYDLISAWIKATRGSDPDAS
                     MLYLVTMLEAGEDPRFIARRMIVLASEDIGLADPRALEIAVAAAHAVEHVGMPECAYN
                     LTEAALYLSLAPKSDTVKRALGATTGWVREHGGADPPDYLRSAAYPGAKKLGHGVGYD
                     YPHERPEGVSPQELMPAGAEDARLVELTEHGEERELRERLERIKKARGR"
     gene            complement(19265..20449)
                     /locus_tag="VF752_06230"
     CDS             complement(19265..20449)
                     /locus_tag="VF752_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEX8855182.1"
                     /translation="MATATPERVPVPRDRENDYTREAADVRRSFLEERTGAKLDHVSQ
                     YSFDPGVAPGNIEHFIGVAQIPVGVAGPLLVNGEHAQGEFYVPLATAEGTLVASYNRG
                     MKLLYDAGGVTTTVMDDKMQRAPAFLFPSAREARAFGEWLKDNFEEIKQVAESTTRSG
                     RLQEIEQYSASRILFTRFNYTTGDAAGQNLTGKATQAACIWINQNYPDIEHYFLESNF
                     ATDKKSSQVNMLRTRGKRVIAEALISNDLLERHMHTTSEMIFKARQVSTLGGFMSGVN
                     NNGAHSANGITAVFIATGQDAANVAESSAALGYAELRENGDYYYSVTIPSLIVATYGG
                     GTGLPTQRECLELLGCYGSGKVRKLAEIVAATVLCGELSLGSAIVAEEWVAAHDLFGR
                     NR"
     gene            20539..21852
                     /locus_tag="VF752_06235"
     CDS             20539..21852
                     /locus_tag="VF752_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8855183.1"
                     /translation="MPAATDLERLTTEYLQRLIRFNTVNPPGNEEPAILWLRDRLEAA
                     GFECELLARTLGRPNLIARLPGRSDGPRLCFLSHVDTVLANPVEWSADPWGGEIRDGC
                     VWGRGALDMKSQTAAEVVAAISLAEDGWRPESGELMVLVVVDEEAGSEDGAKWLCREH
                     PDKVRSDYVINEGGGDVFEFGGKRHYGVCVAEKGVFRFTLRTHGRAGHASIPRIGDNA
                     LKKMAPLIERLSSNRPTLELSPEPEAFMRALGVPVDGDLDAALAAVEKENLLIGILLE
                     PLLGVTVAPTMISASQKINVIPSVAELKVDCRVPPGLGADHARMRVEELLGEGDYEIE
                     FNESTPGNRSPIETPLMNEVRAFIADEDPDADVVPLGLPGYTDSQSFRAAFPGCVAYG
                     WFPQREMSMFEAAPLVHGADERIPISDLGLAARFYARLAPKLLGG"
BASE COUNT         3394 a         7741 c         7554 g         3226 t
ORIGIN      
        1 acctgatcga gcgcctgcgt gaggagcggc cggacgtggc gttcgtcgcc ctccacgggc
       61 gcgacggcga ggacggcacg gtgcaggagc tgctcgagat cctcggcatt ccgtacacgg
      121 gttccggtgt gctggcgtgc atgcgctgca tggacaaagt caccgccaag cacctgatgc
      181 tcgaggcggg catcccgacg ccggacttct tcgcgttcaa cgagacggcg ttccgctcgc
      241 tcggcgccgc cgacgccctg ccggcgatcg aagagcggct gcaattcccg atcgtggtga
      301 agcccgccgc gcagggctcc gcactcggga tcaagttcgc gcgtacgccg aacgacgtcc
      361 cgggtgcgct cgtggccgcg ttctcctacg accagcgcgt gatgctcgag cggcacgtgg
      421 ccggccgcga gctggcggtg tcgatcctcg agggcgccgc actgccgatc gtggaggctg
      481 tgccgacggc cgacgacttc tatgacttcg aggcccgcta cgagatcggg cgcaccgagt
      541 tcatctgccc agcacagctg cccgagacgg tcgccgcgcg tgccgaggcg ctcgccgtct
      601 cgacgtacaa gctgctcggc tgcttcggct tcgcgcgcgt tgacctgatg ctcgaggagg
      661 acaccggcga tctctacgtg ctcgaggcca acgccatccc gggactcacc gagacgagcc
      721 ttctgcccca ggcggccgag gcggccggca tagcgttcga cgcgctggtg gagcggatcc
      781 tcgaactcgc gttcgagcgc gcagcaggcg cgcccgtata gagtcgccgc catggcgatc
      841 aacggacaga gaatcctcat cacaggggca gctcgcggca tcggcgcggg ctccgcgaaa
      901 gccctcgcga agcgcggcgc gcgcgtgtcg ctggtgggtc tcgagcctga agagctcgag
      961 aaggtggccg cggagtgcgg gcctgacgcg atctggttcg aggcggacat cacggaccaa
     1021 gacgcgctcg acgcggccgt tgagggcacc gtcgagaagt tcggagggct cgatgtcgtg
     1081 atctccaacg ccggcatagg cgccggcggc ccgctccagc tgatggagca ggacatgtgg
     1141 gagaaggtcg tcgagatcaa cctgatcggc aacgtgcgca ccatccgcac ctgcctgccg
     1201 cacatcgtcg agagcaaggg ctacgtgctg ccgatcgcct ccgccgccgc gatcgtgcac
     1261 ggcccgctga tgaccgccta ctgcgcggcc aaggccggcc tcgaggccgt cggcaacggc
     1321 ctgcgggtgg agatgaagcc gcacggcgtg gacgtgggcg tcggctactt ctcctggatc
     1381 gacaccgaca tggtgagcgg cggcgacgag catcccttct tcaggaagat gcgcggcgag
     1441 ctgccgggtc cgatgggcaa gacgtacccg ctctccgacg cggtggccgc ggtggtcgac
     1501 ggcgtggagc accggcgccg ctgggtgatg gccccgaagt ggctgcgcgc gatgttcttc
     1561 ctgcgcggcg cggtcgcggc gatcgacaac aagcggatca tcaagatgat gccggaggcc
     1621 gagcacgcgt tccgcgagga gatcgaggcc aagggcgccg acgcgagcgc tcccacgggc
     1681 gcgggcggcg ccgccgccgt gcgcgcgcac tcggccacct agcgactacg ctcgcgcggc
     1741 cgtgaggtcg ctgctcgtgt tcgtgcccgt cctgggcgct ccgatactcc atgcgccggt
     1801 gctggcgctc gacgtgctgc cgggcctcaa gcgcccgctc gacttcggcg cgtcgtggcg
     1861 cggccgccgc atcttcggcg gcaacaagac ctggcgcgga gcgctcttca tggtcgcggg
     1921 cgtgctgatc gccgcgctcg cgctctggca atggcccgcc taccgccacg cgctcccgga
     1981 cgacatcgag aacgcctcgc cggcgttgat cggcgtggtg atcggactcg gagtcgtgct
     2041 aggcgaactc ccgaacagct tcgcgaagcg ccgtctggac ataggcccag gccgccgagc
     2101 gagcggcttg tggggcgtcg cgctgagcgt gtgggaccaa gccgacttcg tgccgctggt
     2161 cgcgctgctc ctcctgccga tctggcagat cccgttcgag gacctgctgc tggcgatcgt
     2221 ggtcgtaggc ggggtccact tcgggatcaa cctgatcggc tatgcgatcg gagcgcggaa
     2281 gacgcttgtg taaggcgatg gatcacccgc gcgcgtcccg gcagaagcgg agtgtggcgg
     2341 ggcccagtac gcggtgctga gcgtgctcgc cagggacccc ggcatgtccg gcgccgacct
     2401 ggcccgggct tgcaacacga cgccccaggc gatgaacggc gtgctcgcga cgctggagcg
     2461 cgacggcctg gtcgagcgcc acccacaccc cacacatggc cgcatccttc aagtcaccct
     2521 gactcgcgaa ggggagcggc gactcaaggc ggccactccc gccgtccgag ggctcgagag
     2581 cacgatcgaa caggacttct cgccagaaca gatcgcagcc gtcaaggcct ggctggtcgc
     2641 ctcagcccag cgcctcgaaa aggccgccac gtcgcgcggg gcatagggaa gagcggggcg
     2701 cgacgggttc ggcaccgagc acgccgctgc tctgcatgga aagcgcaact gcgccggggg
     2761 atactgggcg ggcgatgtcg gagaggacga acgtcgagat cgtgcgccgc gtctatgagg
     2821 cttgggcacg cgacgagctc cctgggccag cgcgcctctt cgacgcggag atcgagtacg
     2881 tgaacccgcc caacgcggta gagccgggca cccgtcacgg gctggccgcc ttcagccggg
     2941 cagtccacga cgcattcgag gggtgggaga cctgggagat agagccggaa cggttcatac
     3001 ccgtgggcga gcgggtcgct gtcgttgtcc gataccgagc ccactggcag acgagcgggg
     3061 ttgatgtggt ggcgcatgag tcggcgctgt ggacggtccg gggtgggaag gtcgtgcgct
     3121 acgagtggtt ccacggcccg cacgacgccc tcgaagccgc cagtctgcgt gagtagccct
     3181 gcggtgtccg ctaagaccta ggagagcggg gcgccggggt aggcggagga gggtccggac
     3241 ctttcccgaa gggcgtcaag agttagggcg aagcccgaac ggagcccgaa gggaaacggt
     3301 caggcccctc cgaacgccgt acagcagcgt cactgctcgc cccccatgaa gtcctcagga
     3361 gtcacgttgt ccaggaagtc cttgaacttc tccacgacct cttccgtgtc ctcgacctca
     3421 tgctcgaact cgatcgcgga ctccgcgatg acctcctcgg cagcaaagat cggcgccgac
     3481 gtccgcaccg caagcgccag cgcatccgac ggccgcgagt cgatctccac gtcgcgcccg
     3541 tcgacccgca gcgtgatcga ggcgtagaac gtgttctcct tcagctcggt gaccgccacg
     3601 cgcgtgcagg cgacctccat ctcgctcagc atgtcgctga tcagatcgtg cgtcatcgga
     3661 cgaggggtgt tggcgccctg aagcttcatc aggatggcgg cggcctccgg gtggccgatc
     3721 cagatcggca ggaacttgtt cccgtcgcgt gtcttcagca ggacgatcgg ctgcttgccg
     3781 accatgtcga agctcactcc gtaaatgacc atttccagca tgtgtattcg cgccttgccc
     3841 ttcgcctggc cgctcccaac gagagtctag acgtgccggc tcgcaactac gctccgtcgc
     3901 cgtgaccctg cctacccgca tcgtcgtctg tacgaacagg cgtccagatc aggtcgcgga
     3961 gtgcctgaca gccctgcgcg cccaagcggg tgacgaggcc ccgcttctgg tgacgagcgg
     4021 gctccgcccg gagcaggtcg agggccatcg tgaggcgttc ccgggcacgg tcatcgagga
     4081 gccgcggccg ggactgtcgc tcgcgcgcaa ccgggcgctc gacgcctgcc ccaacgacgg
     4141 cgtgatcgcc ttcgtggacg atgacgcggt ggttgagccc ggctggtggg aggctctcaa
     4201 gcgacgctgg gaggaggcgc cggacgaggt cgccgtcatc ggcggcccga tccgaccgcg
     4261 ctacgacact ccgccgccgc gctggctctc ccgcccgctg ctcccggcgc tcacgctgct
     4321 cgacttgggc gggcgggtgc tcgacctcga cccggaggtc acgaccgtat acggcgcgaa
     4381 catctcgttc cgcgtcgcgc cgctgcgcga ggtgggcggg ttcgacccgg acttcggcca
     4441 ctccggcaaa cgcgtcttct tctccgagga ggacgaggcg cagcgcgagc tggcgcgcgt
     4501 ggggcacacc gtcaggtacg tgcccgacgc ggcggtgctc cacgtgatcc cgcccgagcg
     4561 gctcacgcgc gcggccttcg tgaggcgccg cttctggtac ggcgtgacgc tcggggcgcg
     4621 gcaggcacgg cccacctggc tcgcgcttcg tcagatggtg agctctgccg ccggggcgcc
     4681 ggtcgcggcc ctccgccgga acgacaggct gttcatggag cgcctcgttc gcgccgccga
     4741 gaacgcgggt gtgctcgccg ctagggctcg atcaggccgc gcctgatcgc gtagcgcacg
     4801 agttccacgc ggtcgcgcat gccgagcttc gcgagcacat tcgcgcggtg tgactccacc
     4861 gtcttctccg acacgtgcag gatctcggcg atctgcttgt tcgtatgcgc ctcggcgatc
     4921 agcttcacca cgtcgagttc gcgtggcgtg agcaggtcgc gcggctgctc tgccccgtcc
     4981 gcaagccact ccttcacgag cacgctctcg ctcgagggcg acaggaactg ctcgccgcgt
     5041 gcgacggcgc ggattgcgtc gatgaggtcg gtgtcggccg cgcgcttcag cacgtagccc
     5101 gcggccccaa ccttgagcgc ctcgaacacg tagcgctcgt cgtcgtgcat cgagagaagc
     5161 aggatcgacg tgcccggaca gtgggcgccg atctcgcgcg cggcctggag ccccgtcatc
     5221 ttcggcatcg agacgtcaag gaccgcgacg tccgggcgca ccgactgggc gagcgagagc
     5281 gcctccacgc cgtcctcggc ctcggccacc acgctcatgc cgtcttggcg ctcgatcagc
     5341 atgcgcagcc cggagcgcac gatgccgtgg tcgtccgcga cgaggatgtg cagcatcagg
     5401 gcacgtgcag ggtcacggtc gtgccctggc cgggagagga gtagatgctg aggtcgccgc
     5461 cgaccaggcg agcgcgttcg gccattccgt tcaggcccag gcccccgccg tccgggcgct
     5521 gggcgtcgaa gccgccgccg tcgtcgcgca cgcagaggtc cactccgccg ttgaccgccg
     5581 agagatcgac gtgcacctgc gaggcacgcg cgtgccgcgc gacgttcgag agcgcctcct
     5641 gcgcgacgcg gtacaccacg agctgctgat cgtccccgag gatgtccggc tcgccgcgcg
     5701 tctggagccg ggcgcggatg ccggtctgct cgccgaagcg ccgcacctgg ctctcgagcg
     5761 cgggcaccag cccgtggtca tcgagcgccg tcgggcggag ctggcgggcg agctggagga
     5821 gctcgtccat cgcctggttc acgagccgct tgagctccgc cacctcctct tcctgctcgg
     5881 gtgacgacga gccctgagcg agcgcctcga gccgcagcag gatggcggtg agcgcctggt
     5941 tcacctcgtc gtggaggtcg cgcgcgagcc gcttgcgctc ctcctcctgc gcgcgcagca
     6001 cgagccggcc gcttcgccgg cgctcgccct cgatgcgatc gagcaggcgc tggaacgagg
     6061 ccaccaggcg ctccacctcg tcagtgccgc cagggggcgg gtcgaagctc gcgggctggg
     6121 ccgggtcgac ggtctcgagc tgctcaatca gacgctcgag cggattgaag cgccgccgca
     6181 gcatcatgat gttcgccgac agcgcgagca cgatcgcgag cgccagcagc gtgaactgca
     6241 ctcgctcgtc gcgcaccgcc aggttgaggc gggacgccat gctcgcggcg aagatcgtcg
     6301 ccgtcacgag aaagacgttg accgcgagga tctggccaat cagggattcg cgcttgttca
     6361 tcgcaaatgg ggtccagacc ccatatcggc aattccgcgc gcgaccgaag acctgggtag
     6421 cgggaggcaa gcgacgaaaa gcctctttag agccgcagca acttgggtcc aacgcggggc
     6481 gccgaaaggc gccccgcgac ttttgacgtc gcagccgctt acgcggactg atccagcgcc
     6541 ggctcgacga cccttcgata tgcgtcgggg tcggtcggcc cctcggtggc gacgaggagg
     6601 acccgcgcgt ccggacccag gccgacggca tcgcggagcg ccgccgcctc gggctccgcg
     6661 gcgagcgcgc gaagggccgc cagcggcgcc gcgccgcagt ccccaatggc aagacccaga
     6721 gcggcgagct cccgcatcgc cgcgtgggct tccgcgtcgc ggatggtgat ggtcccgtgg
     6781 accccagctc gaagcgaggg ccatgccgcc acggacacct ccgcgcagtc gaggcccgcc
     6841 atggtggtgc cgggagtggg cacctcggtc ggctcgccgg ctatcagcga agcactgagg
     6901 caggccgcag tcacgggctc gacgccgatc accttcgcgc cagcctgggc ggcgaagcgc
     6961 gccgcggccg ctgccagtga ccccacgccg gccggcacaa gaatcaggtc gaagggctcg
     7021 cccgcagcga cgacctctgc gaagagcgtg gcgtagccgt cgatgaccca actcgcgggg
     7081 ccggaagctc cgacgtcggc cagttcgagc acaccgtcct gcactccctc ggccgccgcc
     7141 tgtgcaaccg cttcctcata ggttccctcg accaccacga cgtcggctcc ctctgcagcg
     7201 atcccgtccc ggcgtgcctt cgaggccctc gccgggaggt agacgcggca gttcaggccc
     7261 ctgcgcgccg ccacgttggc caccgcacgg ccgtggttgc cggcgcttgc cgcgaccagc
     7321 gtgcgaacgc cctgctgctc gcgaaaggcg cgctcaacgg cccacgacgc gccaagaacc
     7381 ttgaaggcgg gcaacccgag gcggtctgac tcgtccttca gcgagacagc ccgcaggccg
     7441 agctccgcgg cgacgtcccg cagctcgtgg atcggggtgg gctgatagcc ctcgagcgcc
     7501 gcgtggaagg ccgcggcgtc accgctcggc ggcggtacgt ccgcctgccg gacgctgcgg
     7561 ttgacgtaag ccctcacgcg caaagtcggg tgcccgcggc gatcagcgtg cgggtcaccg
     7621 cgacggtgtc ggagatgctc acccactcct cggcggcgtg cgctccctcg ccgccggggc
     7681 cgaacatgac cgtcgggatt cccgcggccg cgatgaacgc ggcgtcggcc caatagctcg
     7741 cgccgccgat cgccggcgcc gacggcagca ctgccgccgc ggcctcgcgt acgacctcca
     7801 cgacgtccgc gtcctggtcc acctcgaagg gctcgcgcac gagcaccgtg cgttgtgtgg
     7861 ccacgagctc cggatccgcg gcgcggcagg agtcgagcag actcgcgagc tcgtcttcca
     7921 cggcccccgc cgtgtcaccc gggagggttc ggcgctcgag gcccagtacg caccgcgccg
     7981 gatagctcga catctccacc ccgccctcga tcaacgatgc gtgcaccgag ccgcgaccca
     8041 gcagcgggtg cgtgcgcgcg ccgagcgccg cgtcgaggtc gccgatcgca gtgaggatcg
     8101 ggcccgtctt gacgatcgcg tcgacgccga ggtgcggtcg tgagccatgc gcagcgcgcc
     8161 cggtcacctc gaactctgcc cacacgaagc ccttgtgggc gaccacgacc tcgagctcgg
     8221 tgggttccgt cacgatcgcc gcgtcggcgc cgattgagct cagcacctcc tgcacgccca
     8281 ggctcgcgtg ctcctcgtcc gccacggctg cgacgaccac gtcgccggcg acgccttccc
     8341 tcgcgagctc gcggcacgcc acaagggccg cggcgagacc cgccttcatg tcataagcgc
     8401 cgcggccata gagccggtcg ccatccactc gcggcgcgtg cgggtccgtc atgccttcca
     8461 cgcccacggt gtccatgtga gcgcatagca gcaggctggc gccgccgccg gtgccgggcg
     8521 cccggacgat cacgctcgga cgcgcgggcg tcccagtggt cagctccgcc tcgaggccgg
     8581 cctggcgagc ccagccagct acgaaggtgg cgatctgcgc ctccccggcc ccgcccggca
     8641 cgagcgaggg gttcacggag tcgatggcga caagctgggt ggtgagcgcg accacgtcat
     8701 cgcgcagcag cgaagcctcc agcgtcccgg ggagtcgctc gtcagacacg tttggtgttc
     8761 agggcttgcg cgagcggcgc cgctcgtcga gcgacgtgac ccgcgcgggc ggacgctcgc
     8821 ggtgctcgcg cctcggctcc gccgccggcg actcgccgtc gtcgcccagc gcgaacaact
     8881 cgcgcagcgc ctgcagctgt gcgtagaagc gtccgtcctc ggcggcgcgc ttcatcctca
     8941 gcgtcggctc gtgcagcagg cggctcacga tcgagcgcgc catcgcctcg atgcgctcga
     9001 agtcggcctc cgacagggac tcccagtgcg gtgagttctc ctgcagcacg tgctcgacga
     9061 tgtcgtcgcc gcgggcgcgc agcgccgcga tcgtcgggac gacgtcgagc gtggccatcc
     9121 actccgcgaa gcgcgccccg tcctgctgga cgagcgcgcg agcccgcgtg gcctcggcct
     9181 cgcggccgct caggttgcgc gccacctcgc gctgcaggtc gtcgatgtcg tacaacgtca
     9241 cgccggagag gtcgcgtacg cgcgggtcga tgtcgcgcgg caccgcgatg tcgatgagca
     9301 gcagcgggcg gcccgcgcgc gtgtccatca cgagctctag ttcgtcgcgc cccacgatcg
     9361 tgtgcggcga cccggtgctc gacacgacga tgtcggcctc catcagctcc acgggcaggt
     9421 cgtcgaagcg caccgccacg ccgccgaagc gctgcgccag cccgatcgca cggtcgtagc
     9481 ggcggttggc cacgaaaacg gtcgacacgc cgcgctctcg cagcgcctgc gccgtgagct
     9541 cgccgttctc gcccgcgccg atcacgagca cgcgccggcg ctcgagctcg cccatcacgc
     9601 cgcgcgcgag gtccaccgcc accgacgaca ccgacactcg cgagcggccc accccggtct
     9661 cggtgcgcac ccgcttgccg gcggcgagcg cgtcgcggaa gaggcggttg gtgatcgggc
     9721 cggtgacgcc ctcgacgagc gcgagctcgt aggcgcgctt cacctgaccc tggatctcgg
     9781 cctcgccgat gatcatcgac tcgagccccg ccgcgacgct gaacaagtgc tctacggcct
     9841 cggtggagcg cagcgaatag aggtgcccga gcagctcggt cgggcgcagc tcggcttggc
     9901 gcgcgagcgc cgagagcacc accgtctccg cctccaccgg gtcgcttgcg acgacgtaga
     9961 gctcggtgcg gttgcaggtg gagatcgaga ccgcctcctc gatggccgga tccgccacga
    10021 gctccacgag cacggagacg gcgcgcccct cgggcagggc aagccgctca cgcacggcca
    10081 cgggcgcagt cttgtgcgac acgcccaggc agaggaggcc ctgcatcact tcccgcgctc
    10141 gtcgagcagc tcgtggaggg actccacctc acgctcgatt ctcgagacct tcatcgccat
    10201 gatcgcgacg tagagcacga ccagggtgag gaagacgatg tacgcagcgg cgacgtactt
    10261 gccggcctcg tcgagcggga gcgccggcac gtcagctccc gatcggctgg gccgggaccg
    10321 tggccgtggg cggcgacggc gctcctccgc gcgacgtccc ctcctcgagc gagagctcga
    10381 ggcggcgccg cagccgcttc agctcgaact tcgcgtgctt cgacacgagc tcgaacttcc
    10441 agagcgtgac gaagagcaaa cccatagcga tcagcgacac gtagaacgtg agccgcatct
    10501 cgccgggcat gttgccgccg gtcgtcgaga gcacgcgggg gtggctgaac gcctgcgcga
    10561 ggcgcaccgc gatgaagttc accggcacga acgcgcccgc cgtgatcgcg aacaccgccg
    10621 cgtagcgcgc ctggcgctcg gggtcctcga tcgagaagcg cagcggctgg tagcagcagt
    10681 agagcaggaa gatgatcagg aacgagacga gcgtcggctc gtcccacacc caccagtggc
    10741 cccaggagcc cttggcccag atcgagcccg tgatgagcac gcccaccgcc agcacgagcg
    10801 acaggtggat cgccacgtac gagcgcatgt cccaggacgg gtcacgcgta cgcaggtact
    10861 tgatcgccat gaccccgccg gcgataaagc cgagcagcgc gacgatcgcg agcggcacgt
    10921 gcaggtagaa gatcttctgg atgaagccct ggtcggcgtc gagcggcgcg tagaagaaca
    10981 ccagcgacaa agacgccgcc atgaagacga ccgaggcagc ggcgaggggg cgaagtccct
    11041 tcgagtacat ccggccacat ggtagaccgc gcgcctaagc gcgccgatgc tcagtcctcg
    11101 agaaggaagt cgtagacggc gtaggccacg agcgcgaaca ccccgtcgta gagcgcgagc
    11161 agcccaagcc acttccccag gtcctccgga tgcgggctga ggctcatcag cggctcggtc
    11221 gcgttggccg ccccgatcac caccggcacg agcaggggca gcaggagcag aggcacgatc
    11281 aggtcgcggg cgcgcgtctc gctcgcgagg cccgacacca gtgcgcccac caccgcgagg
    11341 ccgacatcgg cgagcacgag gaccgcgagt aactccggga acgcgtcgag caggttcggc
    11401 ccgagcaaga ggagtgcgaa cgcgggcagc gccaccacct cgaggacgac gaggtagagc
    11461 aacagcgcgg tcgacttcgc gagaaacacc gcggtgcgat cgatcggcgc gagcaggatt
    11521 ccctcgagcc cgccctgctc gtgctcggcg gcgaacaggc ggttcaccgc gaggatcgtg
    11581 gcgagcagca acgtcaccca cagcacgccc gaggcgaggt cgccgtcgag ctcgttccgc
    11641 ccgagcccga agtggaacag cacgaagacg gtgatcgaga acagcaccat cgggaacacc
    11701 gactcgcgcg tgcgcacctc gatagcgagg tccttgcgca ggatcgctcg ggccgcgcgg
    11761 gtcaccggta gagctcgcgc acctgctcga ccgtgaggcg cccagcgatc acttgacgcc
    11821 cagccctcag accgatcgcc gtgtccgatt ccgccaggcc gtgctccacg tcgtgggtca
    11881 cgagcacgcg agtgcgcccg gaggctcgcc caatcagcgg ctcgagcagc tcggcggcgc
    11941 ccggatcgag ccccgcgcgc ggctcgtcga ggagcagcaa cggcgggtcg tgaagcacag
    12001 cgcgcgcggc ggcgaggcgc tgcaccatcc cgcgcgagag ctcgcgcacg gggtcacccg
    12061 cgcggcgctc catccccacc gacgcgagca cctcctccac gcgctcgcgt gcgacgccgt
    12121 agaggcgagc gtggaacagg agattctcgc ggccgctcag ctcgcggtag agcagcgggt
    12181 cgtgcccgag gaggccgacc tgcccgcgca ccctccagga ctcctgcggc acctcgttgc
    12241 cgagtacgcg caccctgccc gcgtgcgggc gcaggagtgt ggcgagcacc ctcagcagcg
    12301 tcgacttgcc ggcgccgttc tggccgaaga ccccgagggt tgccccggcc ggcagctcga
    12361 acgacacgtc cgtgagcgcc acgcgctccc cgtagcggcg ctcgaggcct accgcctcga
    12421 cggcggtgtc attcacgctc gagcgtctcc gcggcgtgcc gcagcgtcgg ctcgacgagc
    12481 ggccacgact gcgccacgcc cttcggcgaa cccgggaagt tgattatgag cgtgcgcccg
    12541 cgcacacccg agatgccgcg cgtcaggatc ccgagcggcg tgtggttccg cgactccgcg
    12601 cggatcgcct ccgcgtatcc gggcgcctcg cggtcgatcg cgtcgcgcgt ggcctcgggg
    12661 gtcacgtcgt cgagcgtgag ccccgtgcca ccggtggtga agatgaagcg cacgcgctct
    12721 acgtcggcca ggcgcttcag tgccgaggcg attgcgtcgc ggtcgtcgct cacgacctcg
    12781 tggaccgtct cgagtcccgc ctcgttgcac aggtccacga gcgcggggcc cgacacatcg
    12841 tcgccctccc cgcgcgcgag cgtggtcgag atcgtcacca ctgcggcgcg cacgcgcgga
    12901 gcctacgccg gatcgcggcg ccagtcgtgc ctgccgccgc tcttctcgag cagccgcacc
    12961 ggcccgatct ccacgccgcg ctcgagtccc ttcaccatgt cgtacaccgt cagggcggcg
    13021 accgagcagg ccgtcatcgc ctccatctcg acgcccgtct gagcactggt gcgagcctcg
    13081 gcggtgacgc gcacctcgcc cgtgcccgga tcgacctcta tcccgacgtc cacgaatgac
    13141 agcggcaacg gatgacagag cggcacgagc tcagccgttc gcttggcgcc ctggatgccc
    13201 gcaatgcgcg cggtggagac gacgtcgccc ttcggagcat cgccggccgc gacggccgcc
    13261 gccgtctccg gcgacatgcg caccaccgcc tcggcgaccg ccctgcggtc gctcacctgc
    13321 ttgccgccga cgtcgaccat gcgcgcgcgc ccgcgctcgt cgaggtggct gaggcgatcg
    13381 gggtcggcgc tcacgcccga cccgctacgc ggcgcggccg gccgcgagct cctcgcccgc
    13441 cgtccgctgg gccgcggcga tgatgtcgcg gaccgcctgc tcgtcgcctt ggcgcacgag
    13501 ctcgaccggg tccggctcgc cgttcacgat cgactcgaag aagaccttgc ggtcctggta
    13561 ggtgggcagc gtgcccttcg cccagccgcg cacctcgttc agcagctccg cgagacgcgc
    13621 gtagggctcg ccgtactcct cggcgatctc gctcttgatg cgcttcgcga gcgccgggct
    13681 tgcgccggcc gtcgagatcg cgatcgcgag tgggcccgag cggaagatcg ccgggaggat
    13741 gaagttgcag agcggcggaa cgtcgaccac gttcacgagc atggcgcgct cctccgcgtc
    13801 ctcgtagacg cggatgttca cgttcgtgtc actcgtcgcg gcgatcacca tgaaggtgcc
    13861 ctcgaggtcc tcgcggcggt attcgcgccg ctcccaggcg atcgaaccct cggcatccag
    13921 ctcctgcagc ggttcgatcg catgcggcga gatcaggacg accgacgcgt cgcaggcgag
    13981 cagcccctcg accttctcga ggccgatctc accgccgccg accaccacgc agcgccggcc
    14041 gttcagcctc aggcaggcga tgtagaaggg cgtgtcgagc atgtcctgaa cgcagctctg
    14101 gtcgcagatc cccctgcgga gctgacaaac gcattccttg ccggaatagg cctgagcggg
    14161 cattgccact cagtgtaagc cctgctgtgg gccgctcgtt gaacagggga agccaccgtt
    14221 cgggcgacgg tcaccgccaa acaccggccc gatccagaaa ggactcgcat ggtcactcga
    14281 ttgaagcggc tggctctgtt gaccgttgtg ggcgccgcgg catacgccgc gatgccggcg
    14341 gccgccagtg ccgtgacagt tgggcagaac gggaacaatt ttgcctgcgg cggagctggt
    14401 tattcggaga tgcaggtgtc cgttgcagga ccgccggatt acgtggtgcc gtccggcggt
    14461 ggcgtcatca cgtccttccg cgtcctcggc aacgccgata caggcgacac ggtcaagctg
    14521 cagatcttcc gcccggccgg aagcggtaac tacaccgtga ccggcgagag cgcggttcag
    14581 gggccgctga ccccgaacac cctgaacggg ttcaatacac gtgtgcctgt ccgggcggga
    14641 gacatcctgg gcttggccat tgtgactacg aacggcagcc cgccgtgcgt cttctcgacg
    14701 agcagtagcg gagatgtgat cggccagtac aacgtgaccc ccgccgtggg aacgaccggc
    14761 ccgccgagcg cttcggtggg taacctccgc gttaacgtgt cggcagaggt cgaagcggac
    14821 gccgacgatg acggtttcgg cgacgccacg caggatcagt gccccaccga tccgtcgact
    14881 caggggccgt gtccagggcc gacggtgtcg ggcaccgcgg tgaacggaca ggccctcacc
    14941 gcgacgaggg gcggcaaccc gaacaacctg tccttccagt ggctgcgctg cgacgcaagc
    15001 gggaacggct gcgcgccgat tccgggagca acagccgcca gctacacact gacgtttttt
    15061 gacatcggcc atgccattcg cgtgcgtcag ctgcaaagca atgcgagtgg atcgaggaca
    15121 atcgactcag ccgcgaccgc ggcggttgca ccggcgcccg ggaggtgcac gaacgcgcgc
    15181 aacgggaccg ccgggaacga cacgttgctc ggctcgaccg gcggcgacct gatccacgga
    15241 ctcgctgggc gggacagcat caacggcgcc aacggaggcg actgcctcta cggcgatgcc
    15301 ggcaacgaca gggtgaccgg cgccgcgggc ggcgaccggg tatacggcgc caccgggcgc
    15361 gaccggctgt ccggcggggc cggcaacgac cggctcttcg gcggcgccgg cagcgacagg
    15421 atctcggccg gcgccggcaa gaaccgggta tccggcggca gcggtaacga tgtgatcaac
    15481 accgccaatg gccagaggga cactgtgaat tgcggtaccg gcacgagaga caggatccgc
    15541 gctgacagga tcgacaggct gaggggctgc gagatacgga ggctcgtgta gagcgcctac
    15601 cgtcggagca tcctcgctgc gagccgcgcg agtggaattg cgccgcgccg cagcgcgccg
    15661 agcttctgcg tatcgcggcc atacagcagc gtggcggcgg catcgagcgc cttcggcatc
    15721 gacgagtcgt acgggtgcca ccagaagttc cgggcgcgcg ccggatccca ggtcacctgc
    15781 ttcacgttca cgcactcgta gaagccgaac ttcgagtgcg cgcggcccac gcccgattcc
    15841 ttcaccccgc cccacgcgca ctgcatgagg ccgtggctga acatgtggtc gttcagccag
    15901 accatgcccg cctcgagccg gcgtgagatg cgcgcgccct tgtcgcggtc gcacgtccac
    15961 acagacgccc cgaggccgaa gcgggagtcg ttcgcgagct ggatcgcctc ctcctccgag
    16021 tcgacggtca cgatgggcac gaccggaccg aagatctcct cgcgcatgat ccgcatctcg
    16081 ggcgtcaccc cggtcaggac cgccggcgcg aagaagcggc ccggcatcga gctcacatcg
    16141 agcggacccc cgcagagaag ctcggctcca ctcgcgaccg cgtcgtccac gagctcgcgc
    16201 accaggtcga actgcgcttc cgagaccatc ggcccgatct cggtctccgg gtcgagcggg
    16261 tcaccgacgc gcagcgcctt cgcgcccgcc actacgtcgt cgatgaactc ggcggcgacc
    16321 tcacgagcca cgtacacgcg ctcgatcccc gagcaggtct ggccggcatt cgcaaagccg
    16381 gcccagaggc agcccgacac cgcgttgggg agctgggcgt cggccagcac gatctgcggg
    16441 tccttgcccc ccagttcgag cacgcagccc ttcacctgct cggcgcacga cgcagccacg
    16501 ctgcggccgg tctcgaccga gcccgtgaag aacaccttcg ccacgctcga ctcgaccagc
    16561 gcctgcccga ccgcgccgcc cccgtgcacg cagcgcacca ggccctcggg cacgcccgcc
    16621 cgctcgaaca cggactggat gcgctggccg atcagcgggg tgagcggcgc cggcttcagc
    16681 acgacgccgt tgccgcacat gagggccatc gccacctcgc cgaacgggat cgaccagggg
    16741 tagttccacg gcgcgaacac gcccaccacg ccgagcggct cgtaggacag ccaggcgctc
    16801 ttctggcgta cgaagatctg gcgcatcggc acgtcctcgt cggcgagcag gtccggcccg
    16861 ttgtccgcga tccagtgcag gccgtcgatc gtcggcagca actccatcga gtaggactcg
    16921 gagatcggct tgccctgctc gcggctcagc agccgggcca gctcctcgac ttcgtcgacg
    16981 atcgcctgcg ccgcccggcg catgtaggcg gcgcggtcca gcgttggcag ctgcgcccag
    17041 aagggctgca gttccgcgac gtcgtcaaca accgactgga ccgcctccgg agcgatgacg
    17101 ggcaccgcgc cgagccgctc gccggtcgcg ggattgaacg actccagctc cgctggcgcc
    17161 gctgccgtcg cggactccat gcccgggatt ctgcctggga gctactcggg aagtcgcgtg
    17221 tacacgacgc ggttgccgcg ctcgacggtc tgcatcgtgg gcccgagttg ctccatcacc
    17281 tgaagcatgc ctcggtttcc gtccagcatc tcggccttca ggcgggttgc tccacgctgg
    17341 atcgcgagcg acgcgatcgc ctccgcgagc agcgtgccga caccctggcg ctggtgctcg
    17401 tcgtcgacca ccagcccgag ctcggcaagc ccggtctcga gcagggcaat gtccgccagg
    17461 ccgacgatct cctcgacacc ctcgacgggc gcggtggccg cgagcacgag ccgctcgcgc
    17521 gggtcataaa aggtgaagtg ctcgaccaat cggtcctgca cctcgggaac cgacgtgaag
    17581 aagcgacggt ggagcgtctc cgggctcagg cgctcgagga acgcccgcac gcgcggcgcg
    17641 tcactcgccc gtgcgagccg cacgcccagc cgcagacctg agtcgagctc gtgcacgatg
    17701 ccgagcatcg cgccgggatc tacgagctgg cgagctgtct ggggggaagc catgacaatg
    17761 actcaagcag acgcttccgc gcgtgtgagt tagagcctcg taaatgtgac gagacgcact
    17821 gtccgggccc ggaaaagccc tgcattcgcg aagaaggggc agcgctccag ggcaccagga
    17881 cgtgccctaa agcgaccgtc agcgtccgcg ggccttcttt atccgctcca agcgctcgcg
    17941 cagctcgcgt tcctcgccgt gctcggtcag ctccactagt cgcgcatcct ccgctccagc
    18001 gggcatcagc tcctgcggag atacgccctc gggtcgctcg tgcgggtagt cgtagcccac
    18061 gccgtggccg agcttcttcg cgcccggata ggcggcgctt cggaggtagt cgggcggatc
    18121 ggctccgccg tgctcgcgca cccagccggt ggtcgcgccc agggcgcgct tcacggtgtc
    18181 cgacttcggg gcgagcgaca ggtacagcgc agcctcggtg aggttgtagg cgcactcggg
    18241 catgccgacg tgctcgaccg cgtgcgctgc cgccacggcg atctcgagcg cacgcgggtc
    18301 ggccaggccg atgtcctcgc tcgccagcac gatcatgcgg cgcgcgatga agcgcgggtc
    18361 ctcgccggcc tcgagcatcg tcaccaggta gagcatcgag gcgtcggggt ccgagccgcg
    18421 tgttgccttg atccatgccg agatgaggtc gtagtggcgg tcgccgccct tgtcgtagag
    18481 cagcgccttg cgctgcatcg cgtcctcggc gacttcgatc gtgactgctt cgcccgcggc
    18541 cgtctcgacc gccagctcga gcgcagcgag cgccgtgcgt gcgtcgcctc cagagcgggc
    18601 cgcgaggaac tcgagcgcac cgtcgtcgac cggcggggcg tccgggatgc cgcgctcgtc
    18661 gctcagcgag cggcgaagca gcgccagcac gtgcggctcc tccaaggtgc gcagctcgta
    18721 caccgtcgag cgggagagca gcgccgagtt cacctcgaag taggggttct cggtggtcgc
    18781 tccgacgaga accacgagct cctcctccac cgcggggagc agcgcatcct gctgcgcctt
    18841 gttgaagcgg tggatctcgt cgaggaagaa gatcgtgccc tcacccgtcg ttttgcggcg
    18901 gtgctccgcg cgctcgatca cgtcgcgcac ctccgcgcgc ccggcgttca cagccgacag
    18961 ctcctcgaac gcggcacgcg cgttcttggc gagcaggcgg gcgagcgtgg tcttgccggt
    19021 gcccggaggc ccgtagagga tcatcgagtg agggcgcccc tcctcgatcg ccgcgcgtag
    19081 cgaggagccc tcaccaagca ggtgctcctg gccgacgaac tcgccgagcg agatcggtcg
    19141 cattcggacc gcgaggggca ggtgctcagg cggcacggcc gtgcggccgc gcggcgcctc
    19201 gtcagtctcg aagagctttt ccacgccccg attctggcag cagaaggcag cggaaacgcc
    19261 tagttcaccg gttgcgtccg aacaggtcgt gggcggccac ccactcctcg gcgacgatcg
    19321 ccgagccgag cgagagctcg ccacagagaa ccgtggcggc gacgatctcc gcgagcttgc
    19381 gcaccttgcc gctgccgtaa cagccgagca gctcgaggca ttccctctgc gtggggaggc
    19441 cggttccgcc gccgtaggtc gcgacaatca gcgacgggat cgtgaccgag tagtagtagt
    19501 cgccgttctc gcgcagttct gcgtatccga gcgcggccga cgactccgcc acgttcgccg
    19561 cgtcctgccc ggtggcgatg aagaccgcgg tgatcccgtt cgccgaatgc gcaccgttgt
    19621 tgttcacgcc ggacatgaag ccgccgagcg tcgacacctg gcgcgccttg aagatcatct
    19681 cgctggtggt gtgcatgtgc cgctcgagca ggtcgttcga gatcagcgcc tctgcgatca
    19741 cgcgcttgcc gcgcgttcgc agcatgttca cctgcgagct cttcttgtcc gtcgcgaagt
    19801 tcgactcgag gaagtagtgc tcgatgtcgg ggtagttctg gttgatccag atgcacgccg
    19861 cctgggtggc tttgccggtg aggttctggc ccgcggcgtc gcccgtcgtg tagttgaagc
    19921 gcgtgaacag gatgcggctg gctgagtact gctcgatctc ctgaagccgg ccggaccggg
    19981 tcgtactctc cgcgacctgc ttgatctcct cgaagttgtc cttaagccac tcgccgaagg
    20041 ccctggcctc gcgagcgctc gggaagagaa aagctggcgc gcgctgcatc ttgtcgtcca
    20101 tcaccgtcgt agtgacgccg ccggcgtcgt acaggagctt catgccgcgg ttgtagctcg
    20161 cgacgagagt gccctcggcg gtggcgagcg gcacgtagaa ctcgccctgg gcgtgctcgc
    20221 cgttgacgag cagcgggccg gcgaccccga ccggaatttg cgcgacgccg atgaagtgct
    20281 cgatgttgcc cggggcgacc cccgggtcga aggagtactg agagacgtga tcgagcttgg
    20341 cgccggtccg ttcctcgagg aacgatcggc ggacatcagc cgcctcccgc gtgtagtcgt
    20401 tctcccggtc gcgcgggacg gggactcgct cgggtgtcgc ggtggccatg ccggctcctc
    20461 tcgatggctg cctttctgat cgaagtttcc ccgctacagc gccggccgtg gcgcgtttgc
    20521 gatctagact ggcgccggat gccagccgcg accgatctcg agcgcctcac gaccgagtat
    20581 ctgcagcggc tgatccgctt caacacggtg aatccgccgg gcaacgagga gccggcgatc
    20641 ctctggctga gggaccggct cgaggccgcg ggcttcgagt gcgagctcct ggctcgcacg
    20701 ctcggacggc cgaacctgat cgcgcgcctg cccgggcgct ccgacgggcc tcggctctgc
    20761 ttcctcagcc acgtcgacac ggtgcttgca aacccggtcg aatggagcgc cgacccctgg
    20821 ggcggcgaga tacgcgacgg ctgcgtctgg gggcgcggcg cgctcgacat gaagagccag
    20881 accgccgccg aggtggtcgc ggcgatctcg ctcgcggagg acggctggcg ccccgagtcg
    20941 ggcgagctga tggtcctagt ggtggtggac gaggaggcag gctccgagga cggcgcgaag
    21001 tggctctgcc gtgagcaccc cgacaaggtg cgctcggact acgtgatcaa cgagggcggc
    21061 ggcgacgtgt tcgagttcgg cgggaagcgc cactacggcg tgtgcgtcgc cgagaagggc
    21121 gtgtttcgtt tcacgctccg tacgcacggg cgagcgggcc acgcatcgat cccccgcatc
    21181 ggcgacaacg cgctgaagaa gatggcgccg ctgatcgagc gcctctcgtc gaaccggccg
    21241 acgctcgagc tgagcccgga gcccgaggcg ttcatgcgcg cactcggcgt gccggtggac
    21301 ggcgacctcg acgccgcgct cgcggctgtc gagaaggaga acctcctcat cgggatcctg
    21361 ctcgagccgc tgctcggcgt gacggtcgct ccgacgatga tcagtgcgtc gcagaagatc
    21421 aacgtgatcc cgtcggtggc tgagctcaag gtggactgcc gcgtgccgcc cgggctcggt
    21481 gccgatcacg cgcgcatgcg cgtggaggag ctgctcggcg agggcgacta cgagatcgag
    21541 ttcaacgaga gcacccccgg caaccgctcg ccgatcgaga cgccgctgat gaacgaggtg
    21601 cgcgcgttca tcgccgacga ggaccccgac gccgacgtcg tcccgctcgg gctgcccggc
    21661 tacacggact cgcagtcatt ccgcgcggcg ttcccaggtt gcgtggccta cggctggttc
    21721 ccgcagcgcg agatgagcat gttcgaggcg gccccgctcg tgcacggcgc cgacgagcgc
    21781 atcccgatct ccgacctggg tctcgccgca cggttctacg ctcgtcttgc acccaaactg
    21841 cttggcggat gacatgactg aaacacgcaa ggaagagaag ctgcggctcg gcggcatggc
    21901 gctccggaac ggtct
//
LOCUS       DASVXS010000110         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_454831, whole genome shotgun sequence.
ACCESSION   DASVXS010000110 DASVXS010000000
VERSION     DASVXS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454831"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VF752_06240"
     CDS             <1..695
                     /locus_tag="VF752_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855184.1"
                     /translation="RNVHEVVEAAGDKAIFYFPARHDHWLVQTGDGWAAHVAGAIGVS
                     TDAQASWWGGRGVGTVPHGLIAAYGGDTVAAAQAFASRFAGEMNVTVLVDFENDSPRT
                     ALEVADALGDKLWGVRLDTSENLVDRSLWHEMGEFKPTGVNPRLVERVREALDRAGHE
                     RVKIVASGGFTVDKIRSFEEAGVPVDAYGIGSSLLRGSNDYTADVVRVDGRDCAKVGR
                     RYRQNPRLEPVE"
     gene            730..2277
                     /gene="ftsH"
                     /locus_tag="VF752_06245"
     CDS             730..2277
                     /gene="ftsH"
                     /locus_tag="VF752_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8855185.1"
                     /translation="MLTLLGLSMSGFQDWAITWLPIIFMGLIVVLIGVTLRYMPRTKP
                     QEIKPESSESIRWDDVAGVEEAKEELREIVEFLRDPRRFRKLGAKVPKGVLLHGPPGT
                     GKTLLAKAVASEAKAKFFAQSASSFVEMFAGLGAARIRRLFRVARKHKPAIVFIDELD
                     AVGATRGHDISGAKDQTLNQLLVEMDGFGGSDELVVIAASNLLEKLDPALLRPGRFDR
                     QIFVPPPDLRGRQEILKVHSRNKPLQDVDLDLVARQTSGLTGADLANICNEAAIFAGR
                     DHRDVVMMRDFESALERVVAGMQSRRVINDHEKRVVAYHEAGHALCSELLPSVEKVHK
                     ISIIPRGRALGYTMNLPEEDRYLKSKDELLDHLVVLLGGRVSEHVVFGRITTGAADDL
                     KRVHEISRSMVADYGMGTELRSRRLPPDDYSVSDATRRLLDEEQAHLTDLAYRRALAM
                     IVENRPLLDALAEHLLQEEVLERDDIERIMALHRADLLRQMADRELAGGEAHIAASER
                     VDPEAAQ"
     gene            2289..2693
                     /gene="mce"
                     /locus_tag="VF752_06250"
     CDS             2289..2693
                     /gene="mce"
                     /locus_tag="VF752_06250"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX8855186.1"
                     /translation="MFGRIDHIGVVVEDLDAAVELYSKSFQMREQHRETVEAFDVEAV
                     LLEVGDGHVELLRPLSAASGVGKFLERNGPGIHHVAYQTDDIDSTLAAVKAAGIRTID
                     EQPRTGIRNSRVAFLHPKATGGVLTELVEPAH"
     gene            2695..2922
                     /locus_tag="VF752_06255"
     CDS             2695..2922
                     /locus_tag="VF752_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855187.1"
                     /translation="MAEVKRVDVGFSGGQVLALRVQEDAYRGLRDAIDGGKGGWYELP
                     TEDSEVNIDLGQVVYVRLDTETHRVGFSGGS"
     gene            2961..>3299
                     /locus_tag="VF752_06260"
     CDS             2961..>3299
                     /locus_tag="VF752_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8855188.1"
                     /translation="MRTHGHWRPLERTMQAYAHAGEHGKLWLGLRAAGALLDGPRRPL
                     YRRGLRAVGIAYAANQAIKFTVRRPRPRLQDLPPLTRTITALSYPSAHATTAFACART
                     LSRAVPAAPLY"
BASE COUNT          528 a         1116 c         1169 g          486 t
ORIGIN      
        1 tgcgcaacgt gcacgaggtg gtcgaggcgg ccggggacaa ggcgatcttc tacttcccgg
       61 cgcgtcacga ccactggctg gtgcagaccg gggacgggtg ggcggcccac gtcgcgggcg
      121 cgatcggcgt ctccaccgac gcgcaggcgt cgtggtgggg cggccgtggc gtgggcacgg
      181 tgccgcacgg cctgatcgcc gcgtacggcg gggacaccgt ggccgcggcg caggcatttg
      241 ccagtcgctt cgccggcgag atgaacgtga cggtgctcgt ggactttgag aacgactcac
      301 cgcgcacagc gctagaggtg gctgacgcgc tcggggacaa gctctggggc gtgcggctcg
      361 acacctcgga gaacctcgtg gaccgctcgc tgtggcacga gatgggcgag ttcaagccca
      421 cgggcgtgaa tccccgcctc gtggagcgcg tgcgcgaggc gctcgaccgg gcaggccacg
      481 agcgcgtgaa gatcgtcgcg tcgggcggct tcaccgtcga caagatcagg tcgttcgagg
      541 aggccggcgt gccggtcgac gcgtacggca tcggctcgtc gctgctgcgg ggctcgaatg
      601 actacacggc cgacgtggtg cgcgttgatg gacgcgattg cgccaaggtt ggccgcaggt
      661 accgccagaa cccccgtttg gagcctgtcg agtaatgctt ttggggtcac cggccgatga
      721 gataggcaga tgctgacgct tctcggtctt tccatgtccg gattccagga ctgggcgatc
      781 acgtggcttc cgatcatctt catggggctg atcgtggtcc tgatcggcgt gacgctgcgg
      841 tacatgccgc gcaccaagcc gcaggagatc aagccggagt cctccgagtc gatccgctgg
      901 gacgacgtgg cgggcgtcga ggaggccaag gaggagctgc gcgagatcgt cgagttcctg
      961 cgcgacccgc gtcgcttccg caagctcggc gcgaaggttc cgaagggcgt gctgctgcac
     1021 gggccgccgg gcaccggcaa gacgctcctc gccaaggcgg tcgccagcga ggccaaggcc
     1081 aagttcttcg cgcagtcggc ctcgtcgttc gtggagatgt tcgccggtct cggcgccgcg
     1141 cgtatccgcc gcctcttccg cgtcgcgcgc aagcacaagc ccgcgatcgt cttcatcgac
     1201 gagctcgatg cggtcggcgc cacgcgcggt cacgacatct ccggcgcgaa ggaccagacg
     1261 ctcaaccagc tgctcgtgga gatggacggc ttcggcggct ccgacgaact cgtcgtaatc
     1321 gcggcctcga acctgctcga gaagctcgat ccggcgctgc tgcgcccggg acgtttcgac
     1381 cgccagatct tcgtcccgcc gccggacctg cgcgggcgcc aggagatcct caaggtgcac
     1441 tcgcgcaaca agccgctgca ggacgtggac ctggacctcg tggcgcgcca gacgagcggt
     1501 ctcaccggtg cggacctcgc caacatctgc aacgaggcgg cgatcttcgc cggccgcgac
     1561 caccgcgacg tggtgatgat gcgcgatttc gagtcggcgc tcgaacgggt ggtggccggc
     1621 atgcagtcac gccgggtgat caacgaccac gagaagcggg tcgtcgccta ccacgaggct
     1681 gggcacgccc tctgctcgga gctcctcccc tcggtcgaga aggtgcacaa gatctcgatc
     1741 atcccgcgcg gacgcgccct cggctacacg atgaacctgc ccgaggagga ccgctacctg
     1801 aagtcgaagg acgagctgct cgaccacctc gtcgttctgc tcggggggcg cgtgtcggag
     1861 cacgtggtgt tcgggcggat caccaccggc gcggccgacg acctgaagcg cgtccacgag
     1921 atcagccgct cgatggtggc ggactatggc atgggcaccg agctgcgctc gcgccggctg
     1981 ccgccggacg actacagcgt ctccgacgcc acccgccggc tgctcgacga ggagcaggcg
     2041 cacctcaccg acctggcgta ccgccgcgcg cttgcgatga tcgtggagaa ccggccgctg
     2101 ctcgacgcgc tcgccgagca cctgctccag gaggaggtgc tcgagcgcga cgacatcgag
     2161 cgaatcatgg ccctccaccg ggcagacctt ctgcgccaga tggcggaccg ggagctcgcg
     2221 ggcggcgaag cgcacatcgc cgcgagcgag cgcgtggacc cagaagccgc tcagtagtat
     2281 ccgcgccctt gttcgggcgg atcgatcaca tcggagtcgt cgtcgaggac ctcgacgcgg
     2341 ccgtcgagct ctactcgaag agcttccaga tgcgcgagca gcaccgtgag accgtcgagg
     2401 ccttcgacgt ggaagccgtg ctgctcgagg taggcgacgg gcacgtcgag ctgctgcgcc
     2461 cgctgtcggc cgcgagcggc gtgggcaagt tcctggagcg caacggcccg gggatccacc
     2521 acgtcgcgta ccagaccgac gacatcgact cgaccctggc ggccgtgaag gcggcgggga
     2581 tcaggacgat cgacgagcag ccgcggaccg gaattcgaaa cagccgcgtg gccttcctgc
     2641 atccgaaggc gacgggtggg gtactgaccg agctggtgga gccagcacac tgacatggca
     2701 gaagtgaagc gagtagacgt tgggttctcg ggcggccagg tgctcgcgct gcgagtgcag
     2761 gaggacgcct accgcggcct ccgcgacgcc atcgacggcg gcaaaggcgg ctggtacgag
     2821 cttccaaccg aggactcgga agtgaacatc gacctcgggc aggtcgtgta cgtgcgcctc
     2881 gacaccgaga cgcaccgagt cggcttcagc ggcggctcgt aggcctgctg caggccgccg
     2941 acatcgccgt gctgaggctc atgcgcacgc atggccactg gcgaccgctg gagcgcacga
     3001 tgcaggccta cgcgcatgcg ggcgagcacg gcaagctgtg gctcgggctg cgcgccgccg
     3061 gcgcgctgct cgacggcccg cgccgaccgc tctaccgccg cgggctgagg gccgtgggga
     3121 tcgcctacgc ggccaaccaa gcgatcaagt tcaccgtgcg ccgcccgcgg ccacgcctgc
     3181 aggacctgcc accgctcacg cgcacgatca cggcgctcag ctacccgtcg gcgcacgcca
     3241 ccaccgcgtt tgcctgcgcg cgcacgctct cgcgtgccgt gcccgccgcg ccgctctac
//
LOCUS       DASVXS010000111        12008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_950335, whole genome shotgun sequence.
ACCESSION   DASVXS010000111 DASVXS010000000
VERSION     DASVXS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950335"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VF752_06265"
     CDS             <1..1374
                     /locus_tag="VF752_06265"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX8855189.1"
                     /translation="PARAKPGRLRVHHEGHEAARARLAPLRMSARAGVIVTGTEVLTG
                     RVRDANGPWLADQLLELGIELAHVGIVGDRVEDMEAQLRFMDAQDMDLIVTTGGLGPT
                     ADDKTVEVVAQHTGRPLEMDERLFDRIAEILKPMMERWPHLSPEAVLESERKQALVPR
                     GAYVIDPAGTAPGFVVPPREGSAGATIVVMPGPPRELAELWPEAVESDEFQAATRGRS
                     RYETQTLRLFGIPESEIAETLRIAEHEVPRFDDVEITTCLRRGEVEVVARWEPESDGT
                     WAAVRDLIERRHERTLFSSDGASIDEQVAALLQGRRVGLAESCTGGLLAGRLTERPGS
                     SEYMMGSIVSYSNEAKMDLLGVDPALIERHGAVSPEVAEAMADGALARFDADTAVSIT
                     GIAGPGGGTDTKPVGYVCWSVKLSDGRSLARDFRVPGDRREIRDRSTTIALHLLRRLL
                     SGQASPV"
     gene            1352..1975
                     /gene="thpR"
                     /locus_tag="VF752_06270"
     CDS             1352..1975
                     /gene="thpR"
                     /locus_tag="VF752_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX8855190.1"
                     /translation="MGKPRQSEAREGDAAPRGRHGRAGSPRARLFVALDLPEVARREL
                     AVWRDFALRGRDDLRPVPEEALHVTLVFLGYRPEKEIHEIGRTIAGAVEPLPAPTMWA
                     SSVKPVPPRGPRLFAVDLADESGRATLVAEACAMALAGEGYYEPEKRPFWPHITFARV
                     KRDRRAEPIEGMPDGEPFEATQVTLYRSHLSPRGARYEPIEVFELAS"
     gene            1989..2450
                     /locus_tag="VF752_06275"
     CDS             1989..2450
                     /locus_tag="VF752_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855191.1"
                     /translation="MGSEREKIEAELWRHEVQPWVGEQPILELGAFQRVGGIGDVIAG
                     PFSPLVDALLRRRQPLAKPARLPLTFLLAVTPETVHAFHFAHRGEHAVKIRGEVGVWL
                     RGDIRAEVEEGGLSPLILLEWEGGSAECLGGVNARATARLLASPEEGASRD"
     gene            2443..3210
                     /locus_tag="VF752_06280"
     CDS             2443..3210
                     /locus_tag="VF752_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855192.1"
                     /translation="MTDSPPVPDGQRRDGPLVSGQDLHRRFGEGESAVDALRGVSLDI
                     ASGGLTAVMGPSGSGKSTLMHILAGLDRPTSGRVFIDGAEITAMDDNHLTRLRRTHIG
                     FIFQFFNLLPMLNARENILIPLEIAGDKPDQAWVDEVVGKVGLQDRLTHRPSELSGGQ
                     QQRVAIARALVSRPKVIFADEPTGNLDSASSRDILDLLRDSVDSFGQTTVMVTHDPSA
                     AAIADRILFLADGQIVRDQGRSDASTIVAAMAELGAA"
     gene            3207..5792
                     /locus_tag="VF752_06285"
     CDS             3207..5792
                     /locus_tag="VF752_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8855193.1"
                     /translation="MRRIVLKGLAFRKTRAVLTALAVVLGVAMVSGTYVLTDTIKSAF
                     DQIFQGSYKNTSAVISGREFVSESASGNATVPEPLLAKVRKLPGVAAATGQITGVAGA
                     GDQVQLIGKDGKPLSKGGAPSLGFGIDPSLPQFNPLRLTAGTWAIGPGQVVIDKGTAG
                     NEGFKVGDTVPVAADGPRESFRIVGIAKYGNVDSLGGATLAVFDIPTAQRLLRKEGRF
                     DSISMSAKPGVSDQQLVREVRPLLPPSAKVSTGAAEANKASDDTQKFINFIQYFLLAF
                     AVIALFVGSFVIFNTLSITIAQRAREFATMRMIGASRRQVLRSVLLEGFVIGLLASVT
                     GLFLGLGLAKLLNAIFKALSLDLPQAGTVFATRTVVVSLLIGTIITVASSIVPAIRAT
                     RVPPIAAVREGSQVPPTRLSPYSGGFALLLVLASVVALVIGSFANGLSTALTLLLLGV
                     GCLALFVGVALVAKRLVPPIVEVVGQPAERLGGAPGRLARDNAVRNPARTASTAAALM
                     IGLALVTLVATLGAGLRSSDRNALEQQVRADYVVTSDNGFTQFPAAAVKELPAAPGVG
                     VASAVFDDNAKAFGKSIRVDGVDPATIARTYNFQWSQGSDATLAALGPNGAIIKQDYA
                     NDRHLKVGDTFQVTTPSGRRPTFVVRGISKPPVFDKIDPLLGKVIVSQDTFSRFFERP
                     KVLLAFITLDRGVSPQATARLKQALARFPDAKVQSKADWVDERVGGVNQLLNLLYVLL
                     ALSVIVSLFGMVNTLVLAVFERTRELGMLRAVGMTRRQVRRMIRQESVITALIGAALG
                     LPLGLFLAALVTQALQDQGLGFSVPVGSLVAFTVVAVIAGLLAAILPARRASRLDVLQ
                     ALQYE"
     gene            complement(5801..6967)
                     /locus_tag="VF752_06290"
     CDS             complement(5801..6967)
                     /locus_tag="VF752_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX8855194.1"
                     /translation="MTTRADVTEFRVDVPDADLEDLRARLRHARWPERETVVDWSQGV
                     PLAYLRDLCAHWADGYDWRATEARLNALPQFRTVIDGLGVHFIHARSPHRDALPIVIT
                     HGWPGSIVEFLKVIGPLTHPSAHGADAADAFHVVCPSLPGYGFSDKPASQGWGIERIA
                     RAWTVLMSRLGYGRYGAQGSDWGTSISASIAQQDPEHVVGIHLTPPLAPPDPATFDDL
                     SDRERAALESLEQSAEWDSGYSREHATRPQTIGYALVDSPTALCAWIIEKFWAWTDSG
                     GHPENVLTRDELLDNLMLYWVPAAGASSARLYWESIRQVNEWISGPVRDRVTVPTGCS
                     IFPRELQRPSRRWAEKRFLDIRYWNELERGGHFAAFEQPELFVEEVRAFFRLVR"
     gene            complement(6964..7731)
                     /locus_tag="VF752_06295"
     CDS             complement(6964..7731)
                     /locus_tag="VF752_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8855195.1"
                     /translation="MARRDSRRFGPGPYILGLRRGGGWGTGFPFDVPAVAAVETLSLE
                     RPVTLLAGENGSGKSTITEAIAKAIGFADEGGELERSGELPAVPRAVWDGALEPVLSA
                     TRPRNGYFLRAESFFNVAQFVDSGGTFAPDLSLYGDVPLHEQSHGQSFLALAANRFGG
                     EGLYVLDEPEAALSVSGELALLAVIVRAVRAGGQFVVATHSPILLACPDAAIFECDEQ
                     GIERRDYDDVDAVRLTRGFLDAPERFLHAALEDADRT"
     gene            7979..9094
                     /gene="recA"
                     /locus_tag="VF752_06300"
     CDS             7979..9094
                     /gene="recA"
                     /locus_tag="VF752_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX8855196.1"
                     /translation="MDKEQEKAAKARDSALNGALSQIERQFGKGSIMRMGDEDAAVKV
                     SAIPSGALSLDLALGVGGFPRGRIVEIFGPESSGKTTLIYHVLAEAQKLGGICAFIDA
                     EHAMDPAYAKRIGVDVDELLVSQPDHGEQALEIADLLIRSGAIDVVAIDSVAALTPKT
                     ELEGAMGDTTVGLQARMMSQAMRKLAGNLNRANTLCMFTNQIREKVGVMFGSPETQPG
                     GRALKFYSSQRLDIRRIETLKEGVEAVGNRVRVKVVKNKVAAPFRQAEFDIEYGQGIS
                     SEGCLLDLGLEHDIVQKSGSFFSYGDERLGQGRHNAKAFLRENPAMAREIEDKIYTAA
                     GVVPAGRATLAEVPPAEADADAEAPVGEAETAAEQAA"
     gene            9473..10972
                     /gene="rny"
                     /locus_tag="VF752_06305"
     CDS             9473..10972
                     /gene="rny"
                     /locus_tag="VF752_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX8855197.1"
                     /translation="MEIVLAAVVAALVAGGVAVAVRRPRHPAAVGPTAPSEAPAPGEA
                     RAPAPAVVVAPPGDVELELRERRKELARLEERLLSKEQSLDTRLEELARRERTLEDRT
                     RNLDRELERLKAAKQEQIRELERIGGLSAGQARQILMRELEDTVRHDCARVIRQIEEE
                     TRREADRRARNILATTMQRVAGGHAAETTVSVVELQSDDLKGRIIGREGRNIRALETL
                     TGIDFIIDDTPGAVLLSGFDGVRREVARLTLEKLLQDGRIHPARIEEAYYQARSEIDN
                     HIAEVGEQAVFEAGVGAVHPEISKLLGRLHYRTSYGQNVLAHSIECAHLAAMIADELG
                     ASAKTARRAALLHDIGKAVSHEVEGPHALVGGDIARRHKESEAVAHAMEAHHNDVDMQ
                     TVEAVIVQVADSLSGARPGARGESLEQYVKRLRGLEEIAMRHAGVQKVHAMQAGREVR
                     VIVSPEDVDDDTAALLSHKIARDVERELEYPGQIKVTVIRESRAVDYAK"
     gene            11016..>12008
                     /gene="miaB"
                     /locus_tag="VF752_06310"
     CDS             11016..>12008
                     /gene="miaB"
                     /locus_tag="VF752_06310"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX8855198.1"
                     /translation="MQTAPKRYHVTTFGCQMNEHDSERMKGMLESLGYRQADSRDEAD
                     LILFNTCSIRESADNRFIAHLGEAKRLKSEDPEKVVGVGGCWAQSVKDEVFRRFPFVD
                     VAFGPGQIPRLAEFLTSDSLTAQGYFEFESVSGHLPMKREREFQAWVQISQGCNCACS
                     YCIVPSTRGREQSRPAAELVAEIEQLTADDVSEVTLLGQNVNSYGRDLPKGAKTTFSE
                     LLAMVDAIDGVERIRYTSPHPKDMREDVIRAHAELPALCEHIHLPLQAGSSRILKAMR
                     RTYNRERYMDRVAMIRELVPDCAITTDIIVGFPSETEADFEETLEVVDEVGYDSA"
BASE COUNT         1878 a         4095 c         4271 g         1764 t
ORIGIN      
        1 cccgcccggg ccaagcccgg tcggctacga gttcaccacg aagggcacga agccgctcgc
       61 gctcggctcg cgcccctgcg catgagcgcg cgcgccggtg tcatcgtcac cggcaccgag
      121 gtcctgaccg ggcgcgtccg ggacgccaac gggccgtggc tcgccgacca gctgctcgag
      181 ctcggtatcg agctggcgca cgtcggcatc gtcggcgacc gcgtcgagga catggaggcg
      241 cagctccgct tcatggatgc ccaggacatg gacctgatcg tcacgaccgg cgggctcggc
      301 cccacggccg acgacaagac ggtcgaggtg gtggcgcagc acacgggccg cccgctcgag
      361 atggacgagc ggctgttcga ccgcatcgcc gagatcctca agccgatgat ggagcgctgg
      421 cctcatttga gccccgaggc ggtgctcgag tcagagcgta agcaggctct cgtgccgcgc
      481 ggcgcgtacg tgatcgaccc ggccggcacc gcgccgggat tcgtggttcc cccacgcgaa
      541 ggcagcgctg gcgcgacgat cgtcgtcatg cccgggccgc cgcgcgagct ggctgagctg
      601 tggcccgagg cggttgagtc cgacgagttc caggcggcca cgcgcgggcg ctcgcgctac
      661 gagacgcaga ccttgcgcct gttcggcatc ccggagtcgg agatcgccga gacgctgcgc
      721 atcgccgagc acgaggtgcc ccgcttcgac gacgtcgaga tcaccacctg cctccgtcgc
      781 ggcgaggtgg aggtggtggc ccgttgggag ccagaatccg acggcacctg ggcggcggtg
      841 cgcgacctga tcgagcgccg ccacgagcgc acgctgttct cgagcgacgg cgcctcgatc
      901 gacgagcagg tggctgcgct cctgcagggc cgccgcgtcg gcctcgccga gtcctgcacc
      961 ggaggcctgc tggccgggcg cctgacggag cgaccgggct cctcggagta catgatgggc
     1021 agcatcgtct cctactcgaa cgaggcgaaa atggacctgc tcggagtcga ccccgccctg
     1081 atcgagcgcc acggcgccgt ctcccccgag gtggctgagg cgatggccga cggggcgctc
     1141 gcccgcttcg acgccgacac cgcggtatcg atcacgggca ttgccggccc cggaggcggc
     1201 acggacacca agccggtggg ctacgtctgc tggtcggtga agctctcgga cggccgctcg
     1261 ctcgcgcgcg acttccgcgt accgggcgac cgccgcgaga tccgcgaccg atcaaccacg
     1321 atcgcgctgc acctgctgcg gcggctcctc agtgggcaag cctcgccagt ctgaggcgcg
     1381 cgaaggcgac gccgccccgc gcgggcgcca cggccgagcc ggctcgccgc gcgctcggct
     1441 gttcgtggcc ctcgaccttc cggaggtcgc gcgccgcgag ctggcggtct ggcgcgactt
     1501 cgccctgcgc ggccgcgacg acctacggcc ggtgccggag gaggcgctgc acgtgacgct
     1561 cgtgttcctc ggctaccggc ccgagaagga gatccacgag atcgggcgca cgatcgccgg
     1621 cgcggtcgag ccgctgcccg ctccgacgat gtgggcgagc agcgtgaagc cggtgccgcc
     1681 gcgcgggccg cggctcttcg ccgtcgacct ggccgacgaa agcggtcgcg cgacgcttgt
     1741 cgccgaggct tgtgcgatgg cgctcgcggg cgagggctac tacgaacccg agaagcggcc
     1801 gttctggccg cacatcacgt tcgcgcgcgt caagcgcgac cgccgcgccg agccgatcga
     1861 gggcatgcct gacggcgagc cgttcgaggc gacgcaggtc acgctctacc gctcgcatct
     1921 gtcgccgcgc ggcgcgcgtt acgagcccat cgaggtcttc gagctcgcct cctagccttc
     1981 gttcccggat gggatcggag cgcgagaaga tcgaggcgga gctgtggcgc cacgaggtgc
     2041 agccgtgggt cggcgagcag ccgattctcg agctcggcgc cttccagcgc gtcggcggca
     2101 tcggcgacgt gatcgcgggt ccgttctcgc cgctcgtgga cgcgctgctg cggcgccgcc
     2161 agccgctcgc caagccggca cggttgccgc tcacgttcct gctggcggtc acgcccgaga
     2221 ccgttcacgc gttccatttc gcgcaccgcg gagagcatgc ggtgaagatc cgaggcgagg
     2281 tcggcgtgtg gcttcgcgga gacatccgcg cggaggttga ggagggtggg ctgtcgccgc
     2341 tgatcctgct cgaatgggag ggcggaagcg ccgaatgtct tggtggtgtg aacgcgcggg
     2401 caacggctag gctgctcgca tcccccgaag aaggagcgtc ccgtgactga ttcgccccca
     2461 gttccggacg gccagcggcg cgatggcccg ctggtcagcg gccaggacct gcacagacgc
     2521 ttcggcgagg gggagtccgc ggtcgacgcg ctgcgcggcg tcagcttgga catcgcgagc
     2581 ggcggcctca ccgccgtcat gggcccgtcc ggctccggca agtccacgct catgcacatc
     2641 ctcgcgggcc tcgatcggcc gacctcgggg agggtgttca tcgacggggc ggagatcacg
     2701 gcgatggacg acaaccacct cacccggctg cgccgcaccc acatcggctt catcttccag
     2761 ttcttcaacc tgctgccgat gctgaacgcg cgggagaaca tcctgatccc gctcgagatc
     2821 gcgggcgaca agccagatca ggcctgggtc gacgaggtgg tcgggaaggt ggggttgcag
     2881 gaccgactca cgcaccgccc gagcgagctg tcgggtgggc agcagcagcg cgtggcgatc
     2941 gcccgcgcgc tcgtctcgcg gccaaaggtg atcttcgccg acgagccgac cgggaacctg
     3001 gactcggcct cgagccgcga catcctcgac ctgctgcgcg actcggtcga cagcttcgga
     3061 cagacaaccg tgatggtcac gcacgacccg agcgccgccg cgatcgcgga ccggatcctg
     3121 ttcctggccg acggccagat cgtgcgcgac cagggccgct cagacgcgag cacgatcgtg
     3181 gccgcgatgg ccgagctcgg agcggcgtga ggcggatcgt cctcaagggg ctcgcgttcc
     3241 gcaagacgcg ggcggtgctc accgcgctgg cggtcgtgct cggcgtcgcg atggtgagcg
     3301 ggacctacgt cctcaccgac acgatcaagt cggccttcga ccagatcttc cagggctcgt
     3361 acaagaacac gagcgctgtg atctccggtc gcgagttcgt gagcgagtcc gcgagcggca
     3421 atgcgaccgt gccggaaccg ctcctcgcca aggtgcgcaa gctgcccggc gtggcggccg
     3481 cgacggggca gatcacgggg gtcgccggcg ctggcgatca ggtgcagctg atcggcaagg
     3541 acggcaagcc gctgtcgaag ggcggcgcac cgtcgctcgg cttcggcatc gatccgagcc
     3601 tgccgcagtt caacccgctc aggctcacgg ccggcacatg ggcaatcggc cccggccagg
     3661 tggtgatcga caagggcacc gcgggcaacg agggattcaa ggttggcgac accgtgccgg
     3721 tggcggcgga cggcccgcgg gagagcttcc gcatcgtggg catcgccaag tacgggaacg
     3781 tggactcgct gggcggcgcc acgctggcgg tcttcgacat cccgacggcg cagcgcctgc
     3841 tgcgcaagga ggggcgcttc gactcgatct cgatgtccgc caagcccggc gtgagcgacc
     3901 agcagctggt gcgcgaggtt cggccgctgc tgccgcctag cgcgaaggtc tcgaccggcg
     3961 ccgccgaggc gaacaaggcc tccgacgaca cgcagaagtt catcaacttc attcagtact
     4021 tcctgctcgc gttcgccgtg atagcgctgt tcgtgggctc gttcgtgatc ttcaacaccc
     4081 tctcgatcac gatcgcccag cgggcgcgcg agttcgccac gatgcggatg atcggtgcgt
     4141 cgcggcgcca ggtgctgcgc tcggtgctgc tcgaaggctt cgtgatcggg ctgctcgcgt
     4201 cggtcaccgg gctgttcctc ggcctcgggc tcgcgaagct gctgaacgcg atcttcaagg
     4261 cgctgtcgct cgacttaccg caggccggca cggtgttcgc cacgcgcacg gtcgtcgtat
     4321 cgctgctgat cgggacgatc atcacggtgg cgtcgagcat cgtcccggcg atccgcgcga
     4381 cgcgcgtgcc gccgatcgcc gccgtccgtg agggctctca ggtaccgccc acacggctgt
     4441 cgccctactc gggcggattc gcgctgctac tcgtgctcgc gtcggtcgtc gcgctcgtga
     4501 tcggctcgtt cgcgaacggc ctgtcgaccg cgctcacgct gctattgctc ggtgtcggct
     4561 gcctcgcgct gttcgtggga gtggcgctcg tggcgaaacg gctcgtgccc ccgatcgtcg
     4621 aggtggtggg ccagccggct gagcggctcg gcggcgcgcc cggacgcctc gcccgtgaca
     4681 acgccgtgcg gaacccggcg cgcaccgcat cgaccgcagc ggcactgatg atcggcctcg
     4741 cgctggtgac gctcgtggcg acgctcggcg cgggcctgcg cagctcggac cgcaacgcgc
     4801 tcgagcagca ggtgcgcgcg gactacgtcg tcacgtcgga caacggcttc acgcaattcc
     4861 ccgcggcagc ggtgaaggag ctgccggcgg cgcctggcgt gggtgtggcg tcggccgtgt
     4921 tcgacgacaa cgcgaaggcg ttcggcaaga gcattcgcgt ggacggtgtc gatccggcga
     4981 cgatcgcgcg cacctacaac ttccagtgga gtcagggctc cgacgctacg ctcgccgcgc
     5041 tcggcccgaa cggggcgatc atcaagcagg actacgcgaa cgaccgccac ctgaaggtgg
     5101 gcgacacgtt ccaggtgacc acgccgtccg gcaggcgccc gacgttcgtg gtgcgaggga
     5161 tctcgaagcc gccggtcttc gacaagatcg acccgctgct cggcaaggtg atcgtctcgc
     5221 aggacacctt cagccgcttt ttcgagcgac cgaaggtgct gctcgcgttc atcaccctcg
     5281 accgcggcgt gtcgccgcag gcgaccgcga ggctgaagca ggccctcgct cgcttccccg
     5341 acgcgaaggt gcagtcgaag gcggactggg tggacgagcg cgtgggcgga gtgaaccagc
     5401 tgctgaatct gctctacgtg ctgctcgcgc tgtcggtgat cgtgtccctg ttcggcatgg
     5461 tgaacacgct ggtgctcgcg gtgttcgagc gcacgcgcga gctcggcatg ctgcgcgccg
     5521 tgggcatgac gcggcgccag gtgcggcgca tgatccgtca ggagagcgtg atcacggcgc
     5581 tgatcggcgc cgcgctcggg ctgccgctcg gtctcttcct cgcggcgctc gttacgcaag
     5641 ctttgcagga tcagggcctc ggcttctcgg tcccggtcgg ctcgctggtc gcgttcacgg
     5701 tcgtggccgt gatcgccggg ctgctggcgg cgatcctgcc cgcacggcgg gcatcgcggc
     5761 tcgacgtgct gcaggcgctc caatacgagt aggcgccgga tcagcgcacg agccggaaga
     5821 aggcgcgtac ctcctctacg aagagctccg gctgctcgaa ggctgcgaag tggccacccc
     5881 gctcgagttc attccagtag cggatgtcga ggaagcgctt ctccgcccac cggcgcgacg
     5941 gacgctggag ctccctcgga aagatcgagc agccggtggg aacggtgacc ctgtcgcgta
     6001 cgggtcccga gatccactcg ttcacctgcc tgatgctctc ccagtacagc cgcgcggagg
     6061 atgcgccagc agccggcacc caatagagca tcaggttgtc gaggagctcg tcgcgggtga
     6121 gcacgttctc agggtgcccg ccggagtcgg tccatgccca gaatttctcg atgatccagg
     6181 cgcagagggc cgtcggtgag tccacgagcg cgtatccgat ggtctgcggc cgagtggcgt
     6241 gctcgcgcga gtagccggaa tcccactccg cggactgctc gagcgactcg agcgcggctc
     6301 gctcgcggtc gctgaggtcg tcgaaggtgg cggggtctgg cggcgcgagc ggcggggtca
     6361 agtggatgcc cacgacgtgc tcggggtcct gctgggcaat gctcgcgctg atgctcgttc
     6421 cccagtcgct gccctgcgcg ccgtagcggc cgtagccgag ccgggacatc aggaccgtcc
     6481 aggcgcgcgc gatccgctcg attccccaac cttggcttgc cggtttgtcg ctgaagccgt
     6541 accccggcag tgagggacag acgacgtgga aggcgtccgc ggcatcggcg ccgtgagccg
     6601 atgggtgcgt gagcgggccg atgaccttga ggaactcgac gatcgacccc ggccagccgt
     6661 gcgtgatcac gatcggcaga gcgtcgcggt gcggcgatcg agcgtgaatg aagtgaacgc
     6721 cgagcccgtc gatgaccgtc cggaactgcg gaagcgcgtt cagacgcgcc tcggtggctc
     6781 gccagtcgta gccatcggcc cagtgcgcgc agagatcccg caggtaggcg aggggtacgc
     6841 cctgtgacca atctacgacc gtttctcgct ccggccagcg cgcgtgccgc agccgggcgc
     6901 gaaggtcctc gagatccgcg tccgggacgt ccacgcggaa ctccgtcacg tctgcgcgag
     6961 tggtcacgtc cggtccgcgt cctccaaggc cgcatggagg aagcgctcgg gtgcgtcgag
     7021 gaagccccgc gtgaggcgca ccgcgtcgac gtcgtcgtag tcacgtcgct cgatcccctg
     7081 ctcgtcgcac tcgaagatcg cggcgtcggg acaggccagc aggatggggg agtgggtggc
     7141 gaccacgaac tgcccgcccg cgcgtactgc gcgcacgatc acagcgagca gcgccagttc
     7201 gccgctcacc gacagcgccg cctcgggctc gtcgagcacg tagagcccct cgccgccgaa
     7261 gcggttcgcc gcgagagcga ggaacgactg gccgtgcgac tgctcgtgca gcgggacgtc
     7321 gccgtagagg gagaggtcgg gcgcgaacgt gccgccgcta tcgacgaact gcgcgacgtt
     7381 gaagaagctc tctgcgcgga ggaagtagcc gttgcggggc ctggtcgcgg acagcacggg
     7441 ttcgagcgcg ccgtcccaca ccgcgcgcgg cacggcgggc agctctccgg agcgctcgag
     7501 ctcgccgccc tcgtccgcga acccgatggc cttggcgatc gcctcggtga tcgtcgattt
     7561 gccgctcccg ttctcgccgg cgaggagcgt gaccggccgt tcgaggctga gcgtctcgac
     7621 cgccgcgacc gccggcacgt cgaacgggaa ccccgtgccc catccgccgc cacggcgaag
     7681 tccgaggatg tagggccccg ggccgaagcg gcggctatct cgtcgcgcca tcggcgcaac
     7741 cgtaaccgag gccgtccgag ctccggcggc gctcaggcgg cgggcacccc gtcggccgct
     7801 gcttgtagct ccgcgatgtc gagcttcttc atcttcagca tcgcggccat ggcgcgcttg
     7861 gcccgctgtg gtgcattcat gtcacacacc gggttccaaa tcgttacgaa caggtgttcc
     7921 ggtctgtccg atcccgggct tagcctgtcg acctcgccga aatcgtcagg agactgagat
     7981 ggacaaggaa caggaaaagg ccgcaaaggc ccgggattcc gcattgaacg gcgctctttc
     8041 tcagatcgag cgccagttcg gcaagggatc gatcatgcgc atgggcgacg aggacgcggc
     8101 cgtcaaggtc tccgcgatcc cgtcgggtgc gctctcgctc gacctcgccc tcggcgtggg
     8161 aggcttcccg cgcgggcgca tcgtcgagat attcgggccg gagtcgtccg gtaagaccac
     8221 gctgatctac cacgtgctcg cggaggcgca gaagctgggc ggaatctgcg cgttcatcga
     8281 cgccgagcac gcgatggatc cggcctacgc caaacgcatc ggcgtggatg tcgacgagtt
     8341 gctggtgtcg cagcccgacc acggcgagca ggcgctcgag atcgccgacc tgctgatccg
     8401 ctccggcgcg atcgacgtcg tggcgatcga ctcggtcgcg gcgctcacgc cgaagaccga
     8461 gctcgaaggc gcgatgggcg acacgacggt cggcctgcag gcccgcatga tgagccaggc
     8521 catgcgcaag ctggccggca acctgaaccg ggccaacacg ctctgcatgt tcacgaacca
     8581 gatccgcgag aaggtcgggg tcatgtttgg ctcgccggag acccagccgg gtggccgcgc
     8641 gctgaagttc tattcctcgc agcgcctcga catccgccgc atcgagaccc tcaaggaggg
     8701 cgtcgaggcg gtcggtaacc gcgtgcgcgt gaaggtcgtg aagaacaagg tggcggcgcc
     8761 gttccgccag gcggagttcg acatcgaata cggccagggc atctcgtctg agggctgtct
     8821 gctcgatctc gggctcgagc acgacatcgt ccaaaagtcg ggctcgttct tctcctacgg
     8881 cgacgagcgg ctcggccagg ggcgccacaa cgccaaggca ttcctgcgcg agaaccccgc
     8941 catggcgcgc gagatcgagg acaagatcta cacggccgcg ggcgtggtgc ccgccgggcg
     9001 cgccacgctg gcggaggtgc cgccggcgga ggcggacgcg gacgccgagg cccccgtggg
     9061 cgaggccgag acggctgccg agcaggccgc ctagaggtcg gcggccaagt ctgcagaccc
     9121 ccgaagccgt gtgctcggct ccggggggat ttgcccatac acacccggtt gcgcccgtgg
     9181 tgccccaggg gtacgcttcg agcagcggcc ggttcggccg atgcaagaca aaaagccagc
     9241 aaatcagcgc tttctgacgg acaaggcaac cgactgaaat cttgagttct tcacctttga
     9301 cacgaacgca ccgcgaccct ggagggagga tcgagggccg cagctagcgt tgccgcgcgc
     9361 gggcacgcgc gctaaccgcc acagagcacg cctggtgtct tgcggagccg aggctcgccg
     9421 ctggacagct gaactgaccc gggacatgta ggtcccggag gagaccaggg aaatggaaat
     9481 cgtgctggcg gcagttgttg cggcactcgt ggcgggagga gtcgccgtgg ccgttcgccg
     9541 gccgaggcat cccgccgccg ttgggcctac ggcgccaagc gaggcgccgg cgcccggaga
     9601 ggcgcgcgcg cccgctccgg cggtcgtcgt ggcgccgccc ggagacgtcg aactcgagct
     9661 tcgcgagcgg cgcaaggagc tcgctcggct ggaggagcgg cttctgagca aggagcagtc
     9721 gctcgacacg cggctcgagg aacttgcgcg gcgcgagcgc acgctcgagg atcgcacccg
     9781 caatctcgac cgggagctcg agcgcctcaa ggcagccaag caggagcaga tccgcgagct
     9841 cgagcggatc ggcggcctga gtgcaggcca ggcgcgccaa atactcatgc gcgaactcga
     9901 ggacaccgtg cgccacgact gcgcgcgcgt gatccgccag atcgaggagg agacgcgacg
     9961 ggaggccgac cgccgcgctc gcaacatcct cgcgaccacg atgcagcggg tcgcgggcgg
    10021 ccacgcggcg gagacgacgg tctccgtggt tgagcttcag tccgacgacc tgaagggccg
    10081 catcatcggc cgggagggcc gcaacatccg cgcgctcgag acgctcacgg gtatcgactt
    10141 catcatcgac gacaccccgg gcgccgtact gctctcgggc ttcgacggcg tgcggcgcga
    10201 agtggcgcgc ctcacgctcg agaagctcct ccaggacggc cgcatccacc cggcccggat
    10261 cgaggaggcg tactaccagg cgcgctcgga gatcgacaac cacatcgccg aggtggggga
    10321 gcaggccgtg ttcgaggcgg gcgtgggagc ggtgcatccc gagatctcga agctcctcgg
    10381 gcgccttcac taccgcacga gctacggcca gaacgtgctc gcccactcga tcgagtgcgc
    10441 acacctcgcc gcgatgatcg ccgacgagct cggcgcgagc gccaagaccg cccggcgcgc
    10501 ggcgctgctg cacgacatcg gcaaggcggt gtcgcacgag gtggagggcc cgcacgcgct
    10561 ggtcggcggc gacatcgccc gccggcacaa ggagtcggag gcggtcgccc acgcgatgga
    10621 ggcccaccac aacgacgtgg acatgcagac ggtggaggcg gtgatcgtgc aagtcgcgga
    10681 ctcgctctcc ggcgcgcgcc cgggcgcgcg cggcgagtcg ctcgagcagt acgtgaagcg
    10741 gctccgcggc ctcgaggaga tcgcgatgcg ccacgcgggc gtgcagaagg tgcacgcaat
    10801 gcaggccggt cgcgaggtgc gcgtgatcgt cagccccgag gacgtggacg acgacaccgc
    10861 cgcgctcctc agccacaaga tcgcgcgcga cgtggagcgg gagctcgagt accccggcca
    10921 gatcaaggtg acggtgatca gggagagccg cgccgtggac tacgcgaagt aggcgccgca
    10981 gccgcctgag ccgccgccgc taccctgtga gcgcgatgca gactgcgccc aagcgctacc
    11041 acgtcaccac gttcggctgc cagatgaacg agcacgactc cgagcgcatg aagggcatgc
    11101 tcgagtcgct tgggtaccgg caggccgaca gccgcgacga agccgacctg atcctcttca
    11161 acacctgctc gatccgcgag tcggccgaca accgcttcat cgcgcacctc ggcgaggcga
    11221 agcggctgaa gagcgaggac cccgagaagg tggtcggcgt gggcggctgc tgggcgcagt
    11281 ccgtgaagga cgaggtgttc cggcgcttcc cgttcgtcga cgtcgccttc gggccgggcc
    11341 agatcccgcg gctcgccgag ttcctcacga gcgacagcct cacggcgcag ggctacttcg
    11401 agttcgagtc cgtctccggc cacttgccga tgaagcgcga gcgcgagttc caggcttggg
    11461 tgcagatttc gcagggctgc aactgcgcct gctcttactg catcgtgccg tccacgcgcg
    11521 ggcgcgagca gagccgcccg gccgccgagc tcgtcgcgga gatcgagcag ctcacggccg
    11581 acgacgtgag cgaggtgacg ctgctcgggc agaacgtgaa ttcctacggc cgtgaccttc
    11641 cgaagggcgc gaagaccacc ttcagcgagc tgctcgcgat ggtcgacgcg atcgacggcg
    11701 tcgagcgcat ccgctacacg agcccgcacc cgaaggacat gcgcgaggac gtgatccgcg
    11761 cgcatgccga gctgcccgcg ctgtgcgagc acatccacct gccgctccaa gccggctcga
    11821 gccgcatcct caaggcgatg cgccgcacct acaaccgcga gcgctacatg gaccgcgtcg
    11881 cgatgatccg cgagctggtc ccggactgcg cgataacgac cgacatcatc gtcggctttc
    11941 cgagcgagac cgaggccgac ttcgaggaaa cgctcgaggt cgtcgacgag gtgggctacg
    12001 actcggcc
//
LOCUS       DASVXS010000112         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_722264, whole genome shotgun sequence.
ACCESSION   DASVXS010000112 DASVXS010000000
VERSION     DASVXS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722264"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VF752_06315"
     CDS             complement(<1..1106)
                     /locus_tag="VF752_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855199.1"
                     /translation="MTSATVVGAGSFGASIADRLAGSGYEVTLVDQFEPGDSRAASGA
                     ESRLIRGCHGSDEWYTRSARRALDLWRELGDDLFVPSGMTWFARRDDGFEADSERTLR
                     ALGIEVERHPPAAAGELMPGASQEGLAFVLYEPGAGVLRAAECVRALVRRARDRGAEL
                     VRAAARPDGADVLLGDGRRLRSDVVVWAAGAWLGKLFPELVRLRVTQQDVVFFAGPPE
                     WTTPPVPAFVEYDGAAYGLAALDGHGVKIAPDVEGPDFDPNTRERHARAESERLAREY
                     MRVRFPALAAAPLAAATVCQYSLTPDTNFIVAPHPEHDGVWIVGGGSGHGFKHGPALA
                     EYVLELLDGRAVPEPRFGLGARTADRSLRTAGAQS"
     gene            complement(1103..>1821)
                     /locus_tag="VF752_06320"
     CDS             complement(1103..>1821)
                     /locus_tag="VF752_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855200.1"
                     /translation="SGIAGCGTATYSGPDGEGVHVTGTCLDNAGNTGSASFPLNYDAT
                     PPTLTNVTADDGDSEVTLTWVASADTRTIDIVRSASGEPDKSIDNVASGAAAGGPGAY
                     TDTTVSNGTHYTYTVIAHDRANNATTSDPIGADPKAAPKQQVSPVIKKGPKLPVLKWR
                     KVTGARYYNVQLYRGRLKILSAWPRTNKLQLTSSWRYAHKKRRLTSGKYKWYVWPGFG
                     ARKAAKYGKLLKKDTFRFKA"
BASE COUNT          248 a          656 c          633 g          284 t
ORIGIN      
        1 gactgagcgc cggccgtccg taggctgcgg tcggcggttc gcgcgccgag gccgaagcgc
       61 ggctctggca ccgcgcggcc gtcgagcagc tcgagcacgt actccgccag cgcgggaccg
      121 tgcttgaagc cgtggccgga accgccgccg acgatccaga ctccgtcgtg ctccgggtgc
      181 ggcgcaacga tgaagttggt gtcgggtgtg agcgagtatt ggcacaccgt cgcggccgcg
      241 agcggcgcgg cggcgagcgc cgggaaccgc acgcgcatgt actcgcgcgc tagccgctcg
      301 ctctcagcgc gcgcgtggcg ttcgcgagtg tttggatcga agtccggccc ctccacgtcg
      361 ggggcgatct tcacgccgtg cccgtcgagc gcggcgaggc cgtaggcggc gccgtcgtac
      421 tccacgaacg cgggcaccgg cggcgtcgtc cattccggcg ggccggcaaa gaacaccacg
      481 tcctgctgcg tcacgcggag ccggacaagc tcgggaaaga gcttcccgag ccacgcaccc
      541 gcggcccaca ccaccacgtc cgagcgcagc cgacgcccgt ccccgagcag cacgtcggcg
      601 ccatccgggc gggccgcggc gcgcacgagc tccgccccgc gatcgcgcgc ccggcgtacg
      661 agcgcacgca cgcattcggc agcgcgcagc actccggccc cgggctcgta cagcacgaac
      721 gccaggccct cctggctcgc acccggcatc agctcgcctg ccgcagccgg cggatggcgc
      781 tcgacctcga tcccgagcgc gcggagcgtc cgttcgctgt cggcctcgaa gccatcatcg
      841 cgccgcgcga accaggtcat gccgctcggg acgaagaggt cgtcgccgag ctcgcgccag
      901 agatcgagcg cgcgcctagc cgagcgcgtg taccactcgt cgctgccgtg acagccgcgg
      961 atcagccggc tctccgcgcc ggacgcggcg cgcgagtcac ccggctcgaa ctggtcgacc
     1021 agcgtgacct cgtagccgct gcccgcgagc cggtccgcga tcgaggcgcc gaacgagccg
     1081 gcgcccacca ccgtggcgct cgtcacgcct taaacctgaa ggtgtctttc ttcagcagct
     1141 tgccgtactt ggctgccttg cgcgcgccga agcccggcca gacgtaccac ttgtacttgc
     1201 ccgacgtgag cctgcgcttc ttgtgggcgt atcgccagga ggacgtgagc tgcagcttgt
     1261 tggtccgcgg ccaagcactg aggatcttca ggcgcccgcg gtagagctgc acgttgtagt
     1321 agcgggcgcc cgtgaccttc cgccacttga gcaccggcag cttagggccc ttcttgatga
     1381 ccgggctgac ttgctgcttc ggcgctgcct tcgggtcggc accgatcggg tcggacgtcg
     1441 tcgcgttgtt cgcgcgatcg tgtgcgatga ccgtgtacgt gtagtgcgtg ccgttcgaga
     1501 cggtggtgtc ggtgtaggcg ccggggccgc cggcagccgc tccgcttgcg acgttgtcga
     1561 tgctcttgtc gggctcgcca gatgcggaac gaacgatgtc gatggttctc gtgtcggccg
     1621 aggcaaccca cgtgagcgtc acctcggagt cgccgtcgtc cgccgtcacg ttcgtgagcg
     1681 tcggcggcgt ggcgtcgtag ttcagcggga agctcgccga cccggtgttg ccggcgttgt
     1741 caaggcacgt gcccgtcaca tgcacacctt caccgtccgg ccccgagtac gtggccgtcc
     1801 cgcagccggc gatgcccgat g
//
LOCUS       DASVXS010000113         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1218625, whole genome shotgun sequence.
ACCESSION   DASVXS010000113 DASVXS010000000
VERSION     DASVXS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218625"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VF752_06325"
     CDS             <1..333
                     /locus_tag="VF752_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855201.1"
                     /translation="ERCDGEDPLAPFGPNAARHVRRTDGFEHCADIMLNSTYWADTDE
                     VCAFEELVGSHGGMGGTQSYPFLLHPVELELPDEEIVGPEAVHVHLRRWLVQLGHEEY
                     RGELTRVV"
     gene            complement(335..709)
                     /locus_tag="VF752_06330"
     CDS             complement(335..709)
                     /locus_tag="VF752_06330"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX8855202.1"
                     /translation="MIERLKDMPPGTIGFRATDEIEREDYDEVLTPELRRALEAGSGL
                     RTLYLIEDLDEIEPGALWADAKLGWDVGARHRDAWVRSAIVTDIDWMARATKLFAWMI
                     PGEARVFPVAELEQAKAWVAGD"
     gene            748..837
                     /locus_tag="VF752_06335"
     tRNA            748..837
                     /locus_tag="VF752_06335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:782..784,aa:Ser,seq:tga)
     gene            complement(839..1471)
                     /locus_tag="VF752_06340"
     CDS             complement(839..1471)
                     /locus_tag="VF752_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX8855203.1"
                     /translation="MSVPAAARPMTGALRALSKYWWLWLVAGVFWVIVALVILQFDQA
                     SITTVGVLIGIMFLLAAAQQFVVGALTQSGWRWLFWVFGVLFVAAGVISLVHPKNTFA
                     GVADILGFLFLLVGTFWLIEAFAEKDINSLWWLTLVAGILLVILAFWTSGQFQITKVY
                     TLLIFAGIWALTQGITDIVRAFQVRSLRDLPGVEEEPPRATTATPGAMRT"
     gene            1582..2265
                     /locus_tag="VF752_06345"
     CDS             1582..2265
                     /locus_tag="VF752_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855204.1"
                     /translation="METARESRDVILGLAVAGSRVGLATARVVLLPARIVARAPAMDG
                     ALNRVAAQGKRTRDEARRLAEATAQDVLASPEVGRVIDRALAGRLTDSIAASLAEHRV
                     VERVASQMAASGELDRALTAALEHEITQQLADEILASPGFERLLVQVIDSRLLMQLTD
                     RVLLSAEMQRMLDHLVSSPEVRRALAEQTQGMAEEMAAGVRRRTGTIDDVAERTVRGW
                     LRRPRAGVQ"
     gene            2262..2759
                     /locus_tag="VF752_06350"
     CDS             2262..2759
                     /locus_tag="VF752_06350"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX8855205.1"
                     /translation="MNAPRVPYAGIATRAVGLALDVLFADLLVLVTAGVLALVGSLVG
                     LDLSTGLKVAAAGVWFVILAGYFVFFWSTTGQTPGMRLMRLRVAGSDGRPPNFGRAVV
                     RVIGLAVSIIPLFAGFLPVLVDDRRRGLLDFLAGTVVVYADAPFPELERADVAVVAEP
                     LGPPV"
     gene            2785..3039
                     /locus_tag="VF752_06355"
     CDS             2785..3039
                     /locus_tag="VF752_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855206.1"
                     /translation="MDQVIQVIGALLILVAFAATQFGAMSPSSRWYLVLNLVGSVVLS
                     VLAVYEEQWGFVLLETVWALVSAWSLFQVLRGRSPAGAHY"
     gene            complement(3066..3842)
                     /locus_tag="VF752_06360"
     CDS             complement(3066..3842)
                     /locus_tag="VF752_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8855207.1"
                     /translation="MDRSGAGTGTGEVVRIAAAGDIHVRESNRDEMRRAFAEIEEGAD
                     IVLLAGDLTTHGEPEQGKILADACRDLDVPVYAVLGNHDLHVNRASDLIAVLEEAGIH
                     MLERSHAICEVQGVEIGIVGTKGFVGGFPGSHLPDFGEPLLREVYAETTADVEAVDEG
                     LKAVAHCPVRVVLLHYSPTGDTLEGEPPGIWTMLGTDRLAAPIREHEPDLVLHGHAHA
                     GAFRGRIGDVPVFNVSVPVMEQDFWVFEMSGVRRATTPIH"
     gene            3906..>4176
                     /locus_tag="VF752_06365"
     CDS             3906..>4176
                     /locus_tag="VF752_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855208.1"
                     /translation="MAEFHEIVQTTKRAGAALRDADVPFLLGGGLAAWAHGGPESCKD
                     VDLIVREEDAEAALDALGQAGMRPERPPEGWLLKAHDGDVLVDLIF"
BASE COUNT          602 a         1443 c         1456 g          675 t
ORIGIN      
        1 gagcgttgcg acggcgagga cccgctggcg ccgttcgggc cgaacgccgc gcgccacgtc
       61 cggcgcaccg acggtttcga gcattgcgcc gacatcatgt tgaacagcac ctactgggcg
      121 gacacggacg aggtgtgtgc gttcgaggag ctcgtcggct cgcacggcgg catggggggc
      181 acgcagtcgt atccgttcct gctgcacccg gtcgagctgg agctaccgga cgaggagata
      241 gtcgggcccg aggcggtcca cgtgcatctg cgccggtggc tcgtgcagct cgggcacgag
      301 gagtaccggg gcgagctgac gcgagtagtg tgactcagtc gcctgcgacc caggccttcg
      361 cctgctcgag ctcggccacc gggaagacgc gcgcctcgcc ggggatcatc cacgcaaaca
      421 gcttcgtggc gcgcgccatc cagtcgatgt cggtaacgat cgccgagcgc acccacgcgt
      481 cccgatggcg cgcgcccacg tcccacccga gcttcgcgtc ggcccacaac gcaccgggct
      541 cgatctcgtc gaggtcctcg atcaggtaca gggtccgtag cccgctgccg gcctcgagcg
      601 cccggcggag ctccggcgtc agcacctcgt cgtagtcctc gcgctcgatc tcgtctgtgg
      661 cgcggaaacc gatcgtgccc gggggcatgt ccttcagccg ctcgatcatc ggcaggctaa
      721 cctccgccgc gagcggctcg gctccgtgga ggggtggcag agcggttgaa tgcggcggtc
      781 ttgaaaaccg ttaggccggg ttgaccggtc tcgggggttc gaatccctcc ccctccgctc
      841 acgtccgcat agcgcccggc gtcgcggtcg tcgcacgcgg cggctcctct tccacgcccg
      901 gcaggtcgcg cagcgagcgg acctggaagg cacgcacgat gtcggtgatg ccctgcgtca
      961 gcgcccagat gcccgcgaag atcagcaacg tgtagacctt cgtgatctgg aactggccgc
     1021 tcgtccagaa cgcaaggatc acaagcagga tcccggcgac cagggtgagc caccacagcg
     1081 agttgatgtc cttttccgcg aacgcctcaa tcagccagaa ggtccccacc agcaggaata
     1141 ggaaccccag gatgtcggcg acgccggcaa acgtgttctt cgggtgcacg agcgagatca
     1201 cgccggccgc gacgaacagc acgccgaaaa cccagaacag ccacctccag ccggactgcg
     1261 tgagcgcccc gaccacgaat tgctgcgccg cggcgaggag gaacatgatc ccgatcagga
     1321 cgccgacggt cgtgatcgac gcctggtcga actgcagtat caccagcgcc acgatcaccc
     1381 agaagacgcc ggccaccagc cagagccacc agtacttcga gagggcacgc aatgcaccgg
     1441 tcatcggtct tgcggccgcg ggaacgctca ttcggatctc ctttcggttt tcgccaccaa
     1501 tcgtcgtgcg gcgatgggtg gcggtcatca cccggtctgg gtgatgccgc cggccatgct
     1561 gtcggcgatg ctcaacctgc cgtggagacg gcacgtgaat cgcgcgacgt gatccttggg
     1621 ctggccgtgg ccggctctcg cgtcggactg gctacggcgc gggtggtcct cctaccggcc
     1681 cggatcgtcg cgcgcgcccc ggccatggac ggcgcgctga atcgggttgc ggcgcaggga
     1741 aagcgcacgc gtgacgaggc gagacgactt gcggaggcca cggcgcaaga cgtcctcgcg
     1801 tcaccggagg tcgggcgggt gatcgaccgc gcgctcgcgg gtcgcctcac cgattcgatc
     1861 gccgcgtcgc tcgcggagca ccgcgttgtc gagcgcgtgg cttcgcagat ggcggcctcc
     1921 ggcgagctcg accgcgcgct caccgcggct ctcgagcacg agatcacgca gcagctcgcc
     1981 gacgagatcc tcgcgagccc gggattcgag cgcctgctcg tgcaggtgat cgacagccgg
     2041 ttgctgatgc agttgaccga ccgtgtgctg ctcagcgccg agatgcagcg catgctcgac
     2101 catctcgtct cgagccccga ggtgcggcgg gcgctcgcgg agcagacgca gggcatggcc
     2161 gaggagatgg ccgccggcgt gcggcgccgc accggcacga tcgacgacgt tgccgagcgc
     2221 accgtgcgag gctggctgcg gcgcccgcgc gcgggtgtgc agtgaacgcg ccgcgggtcc
     2281 cgtacgcagg catcgccacg cgcgccgtgg ggctcgcgct cgacgtcctg ttcgccgacc
     2341 tcctggtgct cgtgacggcc ggcgtgctcg cactcgtggg gtcgctcgtg ggccttgact
     2401 tgtcgaccgg gctgaaggtc gcggccgcag gcgtatggtt tgtgatcctc gccgggtact
     2461 tcgtcttctt ctggtcgacg accggccaga cgcccggaat gcgcctgatg cgcctccgcg
     2521 tcgcgggcag cgacgggcgt ccgccaaact tcgggcgagc agttgtacgc gtgatcgggc
     2581 tcgccgtctc gatcatcccg ctcttcgccg gcttcctgcc ggtgctcgtc gacgatcggc
     2641 gccgcgggtt gctcgacttc ctcgccggca ccgtggtcgt gtatgccgac gcgccgttcc
     2701 cggagctcga gcgcgccgac gtggccgtcg tggccgagcc gctcggtccg cctgtctgag
     2761 cggcgcctca ctaggctcgg gcacatggat caggtgattc aggtgatcgg cgcgctgctc
     2821 attctcgtgg cgttcgccgc gacgcagttc ggcgcgatgt cgcctagctc gcggtggtat
     2881 ctggtgctca acctcgtcgg ctccgtcgtg ctgtcggtgc tcgccgtgta cgaggagcag
     2941 tggggcttcg tgctgctcga gacggtgtgg gcgctggtgt cggcgtggag cctcttccag
     3001 gtgctgcgcg ggcgctcgcc cgcgggcgct cactattgag agcgggcagg cctcccgcgg
     3061 ggccgctagt ggatcggcgt agtcgcgcgc cgaacgccgg acatctcgaa cacccagaag
     3121 tcctgctcca tcacgggcac tgacacgttg aagaccggca catcgccgat ccgcccgcgg
     3181 aatgcgccgg cgtgcgcgtg gccatgcaac accaggtcgg gctcgtgctc gcgtatgggc
     3241 gcggccagcc ggtcggtgcc gagcatcgtc cagatcccgg gcggctcacc ctcgagcgtg
     3301 tcgccggtgg gtgagtagtg gagcagcacg acgcgcaccg ggcagtgcgc gaccgccttc
     3361 aggccctcgt cgacggcctc tacgtcggcg gtcgtctcgg cgtagacctc gcgtagcagg
     3421 ggctcaccga agtcgggcag gtgggaaccc gggaagccgc cgacgaaccc cttcgtgccg
     3481 acgatcccga tctcgacgcc ctggacctcg cagatcgcat ggcttcgctc gagcatgtgg
     3541 atccccgcct cctcgagcac cgcgatgaga tcggacgcgc ggttcacgtg caggtcgtga
     3601 ttgccgagta cggcatacac cgggacgtcg agatcgcggc aggcgtcggc gaggatcttg
     3661 ccctgctcgg gctcgccgtg cgttgtgaga tcgcccgcga ggagcacgat gtcggctccc
     3721 tcctcgatct ccgcgaatgc gcgcctcatc tcgtcgcgat tcgactcgcg cacgtgaatg
     3781 tcgccggccg ccgcgatccg caccacctcg cccgtgccgg ttccggcccc tgaccggtcc
     3841 atggcgtccc cttaccccgc gtcgccggga cttagaccgc cgccgcggcg ggtattgagg
     3901 ctctcgtggc ggagttccac gaaatcgtgc agaccactaa gcgagccggc gccgcgctcc
     3961 gtgacgcgga cgtgccgttc ctgctcggcg gcggcctcgc cgcctgggcg cacggcggtc
     4021 cggagagctg caaggacgtc gacctgatcg tgcgcgaaga ggatgccgag gcggcgctcg
     4081 acgcgcttgg gcaggcgggc atgcgccccg agcgcccgcc ggagggctgg ctgctgaagg
     4141 cccatgatgg cgacgtgctc gtggacctca tcttcg
//
LOCUS       DASVXS010000114         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1304161, whole genome shotgun sequence.
ACCESSION   DASVXS010000114 DASVXS010000000
VERSION     DASVXS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304161"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF752_06370"
     CDS             complement(<1..547)
                     /locus_tag="VF752_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855209.1"
                     /translation="MFVVTFRTEAPTGVFGPRRRSYSVSVTGGEGRACVVQREVVVPA
                     LPSGRRAEVWLRAAGAGEGTQWCAGPFDGRVLFNDGFACPPAGTCVPPPSAPPTTQQT
                     VGRFALAVSPRAPADVAGFEGRLRACRKPPVDLQVTGVTCRTGRRFYRRFAPAPPGRT
                     EVVLGFTCQVAKVRTSPGLRLT"
     gene            complement(702..1346)
                     /locus_tag="VF752_06375"
     CDS             complement(702..1346)
                     /locus_tag="VF752_06375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX8855210.1"
                     /translation="MAIRDPKVERYLHGLRPERSEVMREMEALAERDGVPIVEWETGR
                     FLATLVRATGARRVLEVGTAIGYSTVHMAEGLVEGGAIVTLEIDEERAAQARSFLDRA
                     GVGDRVEIVLGDARETIPTLDGSWNLIFVDAAKDEYRRYIELAEPQAAERCTLVIDNL
                     LMGGAVAAPESAETHWRPESLAAARELNRELLDSERWLGSVLPIGDGVGFAVRR"
     gene            complement(1451..2062)
                     /gene="cysE"
                     /locus_tag="VF752_06380"
                     /pseudo
     CDS             complement(1451..2062)
                     /gene="cysE"
                     /locus_tag="VF752_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            complement(2089..2862)
                     /locus_tag="VF752_06385"
     CDS             complement(2089..2862)
                     /locus_tag="VF752_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX8855211.1"
                     /translation="MSLSDQELERYSRQLVLPEWSGAAQEQLKSATVVVVGAGALGSP
                     VATYLAGAGVGRLAIVDPDPVELSNLSRQFLHFTPDIEMNKAESAAAKISFLNPEVIA
                     ESYPARVEEDNAASIFLGADVVVDCTDTFESRYVINDACCAIGLPLVEGGVLGLAGMV
                     MSIRPGESACYRCAFPTPPPDDAVPTCQEAGVLGPVAGVVGSIEALEALKLLTGFAEP
                     LTDRILQIDDGGTTQLLVRTSRRDGCPACARVPAATQSP"
     gene            complement(2859..3065)
                     /locus_tag="VF752_06390"
     CDS             complement(2859..3065)
                     /locus_tag="VF752_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX8855212.1"
                     /translation="MTSTLDLTGVPCPMNWVRTKLALESLEPGEELSVALDDGEPIES
                     VPRSAREDGHEVTVQGNIVNIVKR"
BASE COUNT          463 a         1165 c         1112 g          437 t
ORIGIN      
        1 atgtgagccg caagccgggc gaggtgcgga ccttcgcgac ctggcaggtg aagccgagca
       61 cgacctccgt tcgacccggc ggagcgggcg cgaagcggcg gtagaagcgg cgccctgtcc
      121 ggcaggtcac gcccgtcacc tgcagatcga ccggaggctt tcggcaggcg cgcagccgac
      181 cctcgaagcc ggcgacgtcc gccggcgccc gtgggctgac cgccagcgcg aagcgcccga
      241 cggtctgctg ggtcgtcggc ggcgccgacg gcggcggtac gcacgtgccc gcgggcggac
      301 aggcgaaccc atcgttgaac aacacgcgac cgtcgaatgg gccggcgcac cattgcgtgc
      361 cctcgccggc acccgccgcg cgcaaccaca cctcggccct gcgccccgac ggcagcgccg
      421 ggacgactac ctcgcgctgg accacgcagg ctcgtccctc tccgcccgta acgctcaccg
      481 aatagctgcg tcgcctcggc ccgaacacgc cggtcggcgc ctcggttcgg aacgtcacga
      541 cgaacaccga ctgagttccg cccggtccgc gcggaaggac ttgcacgtga ccgactggcg
      601 ggggttggca ccaaagccgg ggccgcgagc gtcaggaacg tcagcagaga ggtgcccaca
      661 tctcccttac gagtcggctg gcggggggag tcccggagcc ctcagcggcg caccgcgaac
      721 cccacgccgt cgccgatcgg cagcaccgag ccgagccagc gctcggagtc gagcagctcg
      781 cggttcagct cgcgcgcggc ggcgagcgac tccgggcgcc agtgggtctc ggcgctctcg
      841 ggcgccgcca cggcgccgcc catcagcagg ttgtcgatca cgagcgtgca ccgctccgcc
      901 gcctgcggct cggcgagttc gatgtagcgg cggtactcgt ccttggcggc gtcgacgaag
      961 atcaggttcc aggaaccgtc gagcgtgggg atggtctcgc gcgcgtcgcc gagcacgatc
     1021 tctacgcggt cgccgacacc agcgcggtca aggaacgagc gcgcctgcgc agcccgctcc
     1081 tcgtcgatct cgagcgtgac gatcgcgccg ccctccacga gcccctcggc catgtgcacg
     1141 gtcgagtagc cgatcgcggt gccgacctcg agaacgcggc gggcgccggt ggcgcggacg
     1201 agcgtggcga ggaagcggcc cgtctcccat tccacgatcg gcacgccgtc gcgctcagcc
     1261 aacgcctcca tctcgcgcat gacctccgag cgctcgggtc gcaggccgtg cagataccgc
     1321 tcgaccttcg ggtcgcgaat cgccacgctc agccgccggc cggatcctgg ccgcggccgc
     1381 cacggatagg ccgcacgtca gcgctcggcg gcggcctgcg cccggtggcc tcctcgagca
     1441 tcgactcgag ctccgtgatg cgctggctga gtgccttgat cgcgtcggcc accggatcgg
     1501 gcaggtgcgc ccagtccgcg tccgggccgg ccggccgctc gccgcgaacg cgcaccgggt
     1561 ggcccgggtt gccgaccacg gtcgagtcct cgggcacgtc gtggatcacg accgagttcg
     1621 cgccgatctt cgagccgcga cttacgtgga tcgggccgag cagcttcgcg ccggagccga
     1681 cgacaacgtc atcgtccacg gtcggatggc gcttgccgcg cgcgaagccc gtgccgccga
     1741 gcgtgacgcc ctggtagagg gtcacgttgt cgcccacctc ggcggtctcg ccgatcacga
     1801 ctccggctcc gtggtcgatg aacagcgcct cccccacctt ggccgccggg tggatctcga
     1861 tgccggtgat cgagcggctc aggtacgcga tcgcgtgagg caccatcggc acgccggtct
     1921 cgtgcagcgc gtgcgcgatc cggtgcgcga gcagcgcgtg cacgccgggg taggtgagca
     1981 ggatctccgc gcgcccaatt ccgcgcgccg ccgggtcgcg ctcctgtgcg gcggtcaggt
     2041 cctcgcgaag ctcgcgcagc acgcgcgtga cggtgcctac gcccaacatc aaggagattg
     2101 tgtcgcagcg ggcactcggg cacacgccgg gcagccgtcg cgccggctcg tgcggaccag
     2161 cagctgcgtg gtgccgccgt cgtcgatctg gagaatgcgg tcggtgagcg gctccgcgaa
     2221 cccggtgagc agcttgagcg cctcgagcgc ctcgatcgat cccaccacgc ccgccacggg
     2281 gccgagcacg ccggcctcct gacaggttgg gacagcgtcg tccggcggcg gcgtggggaa
     2341 cgcgcagcga tagcaggccg actctccggg gcggatcgac atcaccatgc cggccagccc
     2401 gagcacgccg ccttcgacca agggcaggcc gatcgcgcag caggcgtcgt tgattacgta
     2461 ccggctctcg aacgtgtcgg tgcagtcgac gaccacgtcg gcgccgagga agatcgaggc
     2521 ggcgttgtcc tcctcgacgc gcgcgggata cgactccgcg atcacctccg ggttgaggaa
     2581 cgagatcttc gcggccgcac tctcggcctt gttcatttcg atgtccggcg tgaagtggag
     2641 gaactgccgc gacaggttcg agagctcaac cgggtcgggg tccacgatgg ccagacggcc
     2701 gacgcccgcg ccggcgaggt aggtggccac cggcgagccg agcgcgcccg cgccgacgac
     2761 caccacggtc gcgctcttaa gctgctcttg ggctgccccg ctccactcgg gcagcacgag
     2821 ctgacgggag tagcgctcga gctcctggtc cgagaggctc atcgcttcac gatgttgacg
     2881 atgtttccct ggacggtcac ctcgtgtccg tcctcgcgcg cggaccgcgg caccgactcg
     2941 atcggctcgc cgtcgtcgag cgccacgctg agctcctcgc ccggctcgag cgactcgagc
     3001 gccagcttgg tgcgaaccca gttcatcggg cacggcacgc ccgtcaggtc gagcgtcgag
     3061 gtcacggcgc gaagaacggc gtggacacgt agcgctcgcc cgagtccggc acgatcacca
     3121 cgatccgctt gcccgcgaac tccggccggc gcccgagctg gattcccgcc cagagcg
//
LOCUS       DASVXS010000115         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1375474, whole genome shotgun sequence.
ACCESSION   DASVXS010000115 DASVXS010000000
VERSION     DASVXS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375474"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VF752_06395"
     CDS             complement(<1..295)
                     /locus_tag="VF752_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEX8855213.1"
                     /translation="MSTRSDRGQATVITLVFLVVLLAMGALVLDIGSWFRADRATQST
                     ADAAALAGAQALPENPGQAVTLAQRYATSNGGLGTGSVQLSTKIVNNDTIKVTV"
     gene            complement(292..696)
                     /locus_tag="VF752_06400"
     CDS             complement(292..696)
                     /locus_tag="VF752_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX8855214.1"
                     /translation="MTLEIRRKIDLRSEHGQSLTEFALALPILAMLLFAVIQFGIAFN
                     NYVTLTDATRAGARKAAVARQLANPVNACQSAVRSSASDLKQGDLIASCSSSWQMGAD
                     ITVNATYPYKISLLGLVVKSGRLTSTTTERVE"
     gene            complement(777..965)
                     /locus_tag="VF752_06405"
     CDS             complement(777..965)
                     /locus_tag="VF752_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855215.1"
                     /translation="MLKFFTFVQIAADSLRKREEGQTMAEYGVVLAVITLAVIGAITL
                     LSDNVRAAIENVAGILPG"
     gene            1284..>2398
                     /locus_tag="VF752_06410"
     CDS             1284..>2398
                     /locus_tag="VF752_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855216.1"
                     /translation="MYIEIRADVAAHRRPNEREHTAMPRRNSLTRRFLQHLLLISLAR
                     SILTVVGRGRAWDEWDERKLVEPAQKRSFQSRFVQTMSFGALFFAGLALSAGAGNGVR
                     SLLDEDAAVPAAEGASGASGPTGATATAAPVRTVLSSEPARTVVAHEQVAPSRPVVSA
                     APNVGARTAVVSSRSTGAAQLRASTRPSAAGTKRTAVSKTHVARPGQARTRPQHKAPG
                     KHQVAPKAPALDPEVASFSAATVWLNRAAPDPTPPAARLQIAFARQLVASSRKAHVDW
                     ALVLAMLRAEGNNGRSPAGSQALRLLSFRLSSLNAHSTPWAAALAYSSDTAVADRVVA
                     LRHYYRAVGLASLVHGLLSQRADLQDRVLHDSRIVMYP"
BASE COUNT          350 a          830 c          847 g          371 t
ORIGIN      
        1 tgaccgtgac cttgatcgtg tcgttgttca cgatcttggt cgagagctgg acgctgccgg
       61 tgcccagccc cccgttgctg gtggcgtacc gctgtgcgag cgtgaccgcc tggccggggt
      121 tctcgggcag tgcctgcgcg cccgcgagcg cggcggcgtc ggccgtcgac tgggtcgcgc
      181 ggtcggcgcg gaaccacgag ccgatgtcga gcacgagcgc gcccatggcg agcaggacga
      241 cgaggaagac gagggtgatg acggttgctt ggccgcggtc gctgcgagtg ctcattcgac
      301 acgctccgtg gtcgtgctcg tgaggcggcc cgacttcacg acgagcccga gcaggctgat
      361 cttgtagggg taggtcgcgt tgaccgtgat gtccgcgccc atctgccagc tcgacgagca
      421 gctggcgatc agatcgccct gcttgaggtc ggacgccgag ctgcgaactg ccgactggca
      481 cgcgttgacg gggttcgcga gctgccgtgc gacggcggcc ttgcgagcgc cggcgcgcgt
      541 cgcgtccgtg agcgtgacgt agttgttgaa ggcgatcccg aactggatca ccgcgaagag
      601 cagcatcgcg aggatcggca aggccagcgc gaattccgtc agcgactggc cgtgctccga
      661 acgaaggtca attttgcggc gaatttcaag tgtcacggga gtacttcctg tggttcgaaa
      721 gtggagcggg ccccttcgcg gcgaaggggc cccctaccgg agaaactgcg gtggtactag
      781 ccgggcagaa tgccggcgac gttctcgatc gcagcgcgga cgttgtccga gagaagcgtg
      841 atggcaccga tcacggcgag cgtgatcacg gcgagaacca cgccgtactc cgccatcgtc
      901 tgaccctcct cgcgcttgcg gagcgagtcg gcggcgatct gcacgaaggt gaagaacttc
      961 agcatctgtg tcacctccct tcgctgatcg atgcgcacgc gttcgcgcgc gaattcctga
     1021 acggaacaag cgggacggaa ggagaaagct gttgagagac gagccgttcg atcgtgctgg
     1081 cggtgcggtg aatcacgccg gaataacctc cgaagatgtc tcggcgtggc cgcgccgcct
     1141 atggctcagt gcggcgattc atcgttcgca gcggcccggc gggtgatccc tcgtccgggg
     1201 gatcgctcgc ggacttcccc aattcggggg agatggcgcg tgcgcggtga ctcgatagtg
     1261 caccaagtcc gtacggcccg cggatgtaca tcgagatccg cgccgacgtg gcggcccacc
     1321 ggcgcccgaa cgaacgggag cacacggcaa tgccacgacg gaattccctg accaggcggt
     1381 tcctccagca cctcctcctg atctcgctcg cgaggtcgat cctgaccgtg gtcggtcggg
     1441 gtcgcgcgtg ggacgagtgg gacgagcgca agctggtcga gcccgcccag aagcgcagct
     1501 tccagagccg cttcgtccag accatgtcct tcggcgcgct cttcttcgcc ggcctcgcgc
     1561 tctccgcggg cgcgggcaac ggcgtgcgct cactgctcga cgaggatgcg gccgttccgg
     1621 ccgcggaggg cgcgagcggc gcgagcgggc cgaccggcgc gacggcgacg gcagctcccg
     1681 tgagaacggt gctgtcttcg gagccggctc gcacggtcgt cgcccatgag caggtcgcgc
     1741 cctcgcgccc tgttgtctcc gccgcgccga acgtcggggc gcgcaccgcg gtcgtgagct
     1801 cccgctcgac cggcgccgcc cagctgcgcg cgagcacgcg cccgtccgcg gccggcacca
     1861 agcgcacggc ggtctcgaag acgcacgttg cgcggccggg gcaggcacgg acccgcccgc
     1921 agcacaaggc ccccgggaag caccaggtcg ccccgaaggc gcccgcgctc gatccggagg
     1981 tcgcgagctt ctccgccgcg accgtctggc tgaaccgcgc cgcgcccgac ccgacgccgc
     2041 cggccgcccg cctgcagatc gcgttcgcgc ggcagctggt cgcgtcctcg cgcaaggcgc
     2101 acgtcgactg ggcgctcgtg ctcgcgatgc tgcgggccga ggggaacaac ggccgctcgc
     2161 cggccgggtc gcaggcgctc cgcctgctgt ccttccgcct ctcctcgctg aacgcgcact
     2221 cgaccccgtg ggccgcggcg ctcgcgtact ccagcgacac cgccgtcgcc gaccgcgtcg
     2281 tggcgctgcg ccactactac cgcgccgtcg ggctcgcgag cctcgtgcac ggcctcctct
     2341 cgcagagggc cgatctgcag gaccgcgtcc tgcacgactc gcggatcgtg atgtaccc
//
LOCUS       DASVXS010000116         9936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_313897, whole genome shotgun sequence.
ACCESSION   DASVXS010000116 DASVXS010000000
VERSION     DASVXS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313897"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1035)
                     /locus_tag="VF752_06415"
     CDS             complement(76..1035)
                     /locus_tag="VF752_06415"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8855217.1"
                     /translation="MKTMTYEVTGRIARITLDRPERGNGITLDTPRELAECVERADLD
                     PAVHVIALAGNGKGFCGGYDLVESAEQQLPNHDPNEPWDPMLDYSMMSRNVRGFMSLF
                     HANKPVLCKVHGFCVAGGTDMALCSDLLVIEDEARIGYPPARVWGVPTTALWADRIGM
                     QRAKRLLFTGDMIRGRQAVDWGLAIECAPAAELDERFEALLERIAHVPVNQLMLHKLL
                     LNQAAFAQGLHATQVLGTVFDGIARHTPEGYAFQQRAAEAGFKQAVRERDEPFVDSQP
                     KGRQFADHRVPLGLRRPRASGRSGPSTRSSRSRCGRHPATRPS"
     gene            complement(1045..1755)
                     /locus_tag="VF752_06420"
     CDS             complement(1045..1755)
                     /locus_tag="VF752_06420"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX8855218.1"
                     /translation="MERGAWRQIFAIGGGAFAEREGPLLAEHVLSLARGERPKVCFIP
                     TAGGDSPDGIVAFYRLMWQFPCVPFDLQLFPRTVTDLRGFVLAQDVLWVGGGSTANML
                     AVWRVHGLDEILREAYEQGIVLTGSSAGMNCWFEASVTDSFSPTELAGLDDGLGFLEG
                     SACPHFDGEPLRRPTYHRLVADGFPPGIAADDHVGVHYVDERLEQVVSARPGATAYRV
                     EARDGGATETPLDAVELT"
     gene            1795..2181
                     /locus_tag="VF752_06425"
     CDS             1795..2181
                     /locus_tag="VF752_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855219.1"
                     /translation="MDQAVVLDHVVIAVSDWEQSNAFYRDVLGAEVVDLPGGRFAYRL
                     GETQLNLHGPGVVASPLAADPVRPGNSDLCFAWPGPIEEAVAHLASQEVEVEEGPVER
                     NGAAGRGRSVYFRDPDGSLLEFISYR"
     gene            complement(2329..3936)
                     /locus_tag="VF752_06430"
     CDS             complement(2329..3936)
                     /locus_tag="VF752_06430"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8855220.1"
                     /translation="MTTVDRGSTYSTHEVTNQSKPLLGYNVFDADRALAEALEREGGG
                     WGLDRVRDLGAVAGSEEADEHSRRCERNEPRLLTHDRFGNRIDQIDLDPSWHWILRLG
                     VEREVPSLPWREERAGAHVVRAALMFVFSQINAGVSCPVSMTYSGVPALRAEPELAAE
                     WEPLLTLPDYERGALCGMAMTEKQGGSDVRANTTRAEPAGDGWWEITGHKWFCSYPPC
                     DVFLVLAQTQSGVSCFLVEGPGPEQPGFRIQRLKDKLGTRSLPSSEVEFHSVPARLVG
                     EEGRGVRTIIEMVNHTRLDCLIGSAAGMRRGVAEAVNHTRQRSAFGKPLAEQPAMMNV
                     LADLHIESEAATAVAMRIARAYDEKDRPFKRFATAVEKYWVCKRAAPHANEALECFGG
                     NGYVEEAPMARLYRDAPLNSIWEGSGNVAALDVLRAFSKEPEGLQAFLAECELARGAD
                     PRLDTHLDSLPKRLEGLGDGDPQWQARALVEDLGVALQASLLVRNAPSAVADAFCAGR
                     LGDGGRAFGTLPAGVDAKALVDRALAI"
     gene            complement(3951..4586)
                     /locus_tag="VF752_06435"
     CDS             complement(3951..4586)
                     /locus_tag="VF752_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8855221.1"
                     /translation="MAYRHTEKTRARQAAVRERIVAAALDRVAEGGYASASVQAVAAR
                     AGVATGTVYRHFPSKADLFAEVFRRATEREVDVVAEVTGGEGPVLERIARAIEAFSRR
                     ALAGPVLAYALIAEPVDPAVEAERLRYRHAYRDVFAAALAEGVESRELRPHDSGVVAA
                     ALVGALAEALVGPLSPAPEDHRQTDALVANVVQFCVGGIAAETKIDRPVAA"
     gene            complement(4791..5558)
                     /locus_tag="VF752_06440"
     CDS             complement(4791..5558)
                     /locus_tag="VF752_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855222.1"
                     /translation="MPWTRRTFRALLLAASALAALASQAHASNTQTLDTGVELLKQSP
                     GGPWDIGLNIVAGIGTPDGSKPVPLKKAVFLFPHATVNTKAFPTCDAVKLQKEPTVRT
                     CPRASRLGSGEALVDARPFINPERATITMFNGKPKGGKPHFIFLGMAEHFEINLVIDA
                     TLEKVNGRFGYKLSMQFPDIPTLPHNPNASISKLDVNVKAFGKLRGKRVSLLQAPTSC
                     PRGGWRFGSTFGYADGSTTSAASTIDCILRGRPEPVS"
     gene            5620..6324
                     /locus_tag="VF752_06445"
     CDS             5620..6324
                     /locus_tag="VF752_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855223.1"
                     /translation="MRPAGLAILGALLLALPATAAAAPPDTRITAGPDKYTTSKTPQF
                     AFESTVPGSTFECRVNYAGWAPCTSPFTVDARYIATYVFEARAIGPDGDPDPTAAFRR
                     FHRGDIFPPVVKLTRFHSKMKIKSFKRLSGTAVGTLGIGSVQVSMRDADKRRLLPHQE
                     TEQCISVDLETGQRSWRYCSFPQYVRARGGAKWALPISRKVREHLKPGRYEIWVRATN
                     VLGNGEVTKFFVTLKR"
     gene            6580..7953
                     /locus_tag="VF752_06450"
     CDS             6580..7953
                     /locus_tag="VF752_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855224.1"
                     /translation="MSKPRSLLRLLGVMVLAIAVGITFAGPASAKQSKAQKRASKVLI
                     KQLKKNPRLIKSKRWLRKAAHVQATLPLTVRLNPIINKSATPGDTTEAASDDTANIDL
                     TSTFGPNVGNKVTKLSGKLDVLATFGNPAEGDDLGDLRLSVTSASLSASSVAVLDTPG
                     TGAACAADNGGAGAAARPDNTLTTGGLGDRSTLGNFVSAASQAPSVTPDTTVRTAPIS
                     INLASVAGSPAIGKANIFSDTNNVRLSLHAAAKVNTIFRSTETGMGFAAGQLPGAAFF
                     CDEAYAGQTDSSGLGTPPGSPTTGGDIGPVGHRNIIPVNVTGNLKINPAITTDGKLRL
                     ATVTVQSTRPEESTIDACLQINSLIRTADSSAASNTLLGSPSGFALLTRNATPTTACD
                     SAYTPYNPATATGTEPWPLAAVIDAGGDTLADEITPASAKFLQLDPNVEVTGLSAEAL
                     VGTGASY"
     gene            8129..9322
                     /locus_tag="VF752_06455"
     CDS             8129..9322
                     /locus_tag="VF752_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855225.1"
                     /translation="MRFLRERHARFVLAVDLRPAARIALVAAATALALMAVGAGRAAA
                     STSAAESQARALVAQNAAHGQRAQQSAKKKKKKKKLCVVKKLVNGKLRTVYIRTYAYR
                     YKKKGGKKVRTIVRVKKAMRAACTKHCVKQVTKNGKVRTVYVTRKVTVLVKKGNKLVK
                     RKRKMRMPALAKCPSTSGETVVGTPIKIKLIPGNPDTGSGSFARLDFGSFQRDAALSG
                     EITGFSPGRIDISKPVDFTLTGGAINLAPTGIFIDDECFGEVSSAIRTGPGTNITLNK
                     ARENSGNLDPDANVVTSVINVKIRASLELRNDVDGCHKPYLPTGYTDTNARFLLRGAL
                     DSSGGLRVRLASTSVLLEDFTACLALGPANLPCNGFAAPLPFLVSNKVLASLEFGNYG
                     KIKVS"
     gene            9319..>9936
                     /locus_tag="VF752_06460"
     CDS             9319..>9936
                     /locus_tag="VF752_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8855226.1"
                     /translation="MRWRATLLAAGAAAVALTSQAAAGERAVVSQGGEPRLLVALTPG
                     VAPSRVPGLRDGRLVSRALNAWTATAGGSADPAALARSVARRRGVVAAQPDTLIRRAQ
                     AGITCSGAPGTPDGYLIGAVRAGGAPLDATRPVAVVDSGIDGSLDEFAGKVVKPVNVV
                     SGGADVTDGDGHGTGVAGVAAARAGGMRGVAQGSPVMPVKMFGADG"
BASE COUNT         1580 a         3475 c         3369 g         1512 t
ORIGIN      
        1 cccggccgtg tgacgtcgcg tcggccatgc cgggcatcgc cgcgtcgatc gtctcgagac
       61 gctcgtgcac gagcgtcagc tcggtcgcgt cgccgggtgc ctcccgcagc gtgatcgtga
      121 gctgcgagtc gaaggcccgg atcggccact ggctcggggc cgacgaagcc ccagcggcac
      181 acgatgatca gcgaattgtc gaccttttgg ttgagagtct acgaacggct cgtcgcgctc
      241 gcgcaccgcc tgcttgaagc ccgcctcggc cgcgcgctgc tggaacgcgt agccctccgg
      301 tgtgtggcgc gcaatgccgt cgaagacggt gccaaggacc tgcgtcgcgt gcagaccctg
      361 cgcgaacgcc gcttggttca ggagcagttt gtggagcatg agctggttca ccggcacgtg
      421 agcgatgcgc tcgagcagcg cctcgaagcg ctcgtcgagc tcggccgccg gcgcgcactc
      481 gatcgcgagg ccccagtcca cggcttggcg ccccctgatc atgtcgccgg tgaacagcag
      541 ccgcttcgcg cgctgcatgc cgatccggtc ggcccacagc gcagtcgtcg gcacgcccca
      601 gacgcgcgcg ggcgggtagc cgatgcgcgc ctcgtcctcg atcacgagca ggtccgagca
      661 cagcgccatg tccgttccac ccgccacgca gaagccgtgc accttgcaca gcacgggctt
      721 gttggcgtgg aacaggctca tgaagccgcg cacgttgcgg ctcatcatcg agtagtcgag
      781 catcgggtcc cacggctcgt tcgggtcgtg gttgggcagc tgctgctccg cgctctcgac
      841 gaggtcgtag ccgccgcaga agcctttgcc attgcctgcg agggcgatca cgtgtacggc
      901 gggatcgaga tcggcccgct ccacgcattc ggccagctcg cgaggtgtgt cgagcgtgat
      961 gccgttgccc cgctcgggtc ggtctagcgt gatccgggcg atgcgcccgg tcacctcgta
     1021 ggtcatcgtc ttcatcggcg cagcctatgt gagctcgacc gcatcgagcg gggtctcggt
     1081 cgcgccgccg tctcgcgcct ccacgcggta cgcggtcgcg cctggccgag cgctcacgac
     1141 ctgctcgagc cgctcgtcca cgtagtgaac gcccacgtgg tcgtcagccg cgatgccggg
     1201 cgggaagccg tcggccacga gccggtggta ggtgggccgc cgcagcggct cgccgtcgaa
     1261 gtgcgggcat gcgctgccct cgaggaagcc gaggccatcg tcgagaccgg cgagctcagt
     1321 gggggagaac gagtccgtga cggaggcctc gaaccagcag ttcatgcccg cgctcgaacc
     1381 ggtgagcacg atgccctgct cgtaggcctc gcggaggatc tcgtcgagcc cgtgcacgcg
     1441 ccacaccgcg agcatgttcg ccgtgctgcc cccgcccacc cagagcacgt cctgcgcgag
     1501 cacgaagcca cgcaggtcgg tgacggtgcg cggaaacagc tgcaggtcga acggcacgca
     1561 agggaactgc cacatgagcc ggtagaacgc cacgatgccg tcgggcgagt cgccgccggc
     1621 cgtcgggatg aagcacacct tcggtcgctc gccgcgcgcg agcgagagga cgtgctcggc
     1681 cagcagcggc ccctcacgct ccgcgaacgc accaccgccg atcgcgaaga tctgcctcca
     1741 tgctcctcgc tccatccgta agcgaaccta acggaaaacg gaaaggggaa gtgaatggat
     1801 caggccgttg tgctcgatca cgtggtgata gccgtgagcg actgggagca atcgaacgcg
     1861 ttctaccgcg acgtgctcgg tgcggaggtc gtcgacctgc caggagggcg cttcgcttac
     1921 cggctcggcg agacccagct caacctgcac gggccgggcg tggtggcgtc accgctcgcg
     1981 gccgacccgg tgcgtcccgg caactccgac ctgtgcttcg cgtggccggg gccgatcgag
     2041 gaggcggtgg cgcacctcgc gtcgcaagag gtggaggtcg aggagggacc ggtcgagcgg
     2101 aacggcgcgg ctgggcgcgg gcgcagcgtc tacttccgcg acccagacgg gtcgctgctg
     2161 gagttcatct cgtatcggta gctgggcgcg ctccgctcgc gtcgccgcgg tactcggcgc
     2221 gtcgcccggt gtagcactta ggggtggcga acgcgaagtg tggaactttt acccgcctat
     2281 agccgggtca atgttccaca gatcggcctc gggccgccct ttattgccct aaatcgccag
     2341 cgcccgatcc acgagcgcct tcgcatcgac gcccgcgggc agagtgccga acgcgcggcc
     2401 gccgtctccg agccgccccg cgcagaacgc atccgccacg gcggatggcg cgtttcgcac
     2461 cagcaggctc gcctgcagcg ccacgccgag gtcctccacg agcgcgcgcg cctgccactg
     2521 cgggtcgccg tcgccgagcc cctcgagccg cttcggcagc gagtcgagat gcgtgtcgag
     2581 ccgaggatcg gcgccacgcg cgagctcgca ctcggccagg aaagcctgca gtccctcggg
     2641 ctccttcgag aacgcgcgca ggacatcgag cgcggcgacg ttgcccgatc cctcccagat
     2701 cgagttgagc ggagcgtcgc ggtagaggcg cgccatcggc gcctcctcca cgtagccgtt
     2761 gccgccgaag cactcgagcg cctcgttcgc gtgcggcgcg gcgcgcttgc agacccagta
     2821 cttctccacg gcggtcgcga agcgcttgaa gggccggtcc ttctcgtcgt aggcccgcgc
     2881 gattcgcatc gccacggcgg tcgcggcctc ggactcgatg tgcaggtccg cgagcacgtt
     2941 catcatggcc ggctgctcgg cgagcggctt gccgaacgcc gagcgctggc gcgtgtggtt
     3001 cacggcctcg gccactccgc ggcgcatgcc ggcggcgctg ccgatcaggc agtcgagccg
     3061 cgtgtggttg accatctcga tgatcgtccg cacgccgcgg ccctcctcgc ccacgagccg
     3121 ggcgggcacg ctgtggaact ccacctcgga ggagggcagc gagcgcgtgc cgagcttgtc
     3181 cttcagccgc tggatgcgga agcccggctg ctccgggccc ggcccctcga cgaggaagca
     3241 cgatacgccg gactgcgtct gcgcgagaac gaggaagacg tcgcacggcg ggtacgagca
     3301 gaaccacttg tgaccggtga tctcccacca gccgtcgccg gcgggctccg cgcgggtggt
     3361 gttagcgcgc acgtcggagc cgccctgctt ctccgtcatc gccatgccgc agagcgcgcc
     3421 gcgctcgtag tcgggcagcg tcagcagcgg ctcccactcg gccgcgagct ccggctcggc
     3481 gcgcagggcg ggcacgcccg agtacgtcat cgagaccggg catgacacgc ccgcgttgat
     3541 ctgcgagaag acgaacatca gcgcggcgcg aaccacgtgc gcgcccgcgc gttcctcccg
     3601 ccacggcagc gacggcacct cgcgctcgac gcccagccgg aggatccagt gccaggacgg
     3661 gtcgaggtca atctggtcga tccggttgcc aaagcggtcg tgcgtcagga gccggggctc
     3721 gttccgctcg cagcggcgcg agtgctcatc ggcctcctcg gagccggcga ccgcgccgag
     3781 atcgcgcacg cggtcgagtc cccagccgcc gccctcgcgc tcgagcgcct ccgcgagcgc
     3841 gcggtcggcg tcgaagacgt tgtagccgag cagcggcttc gactggttcg tgacctcatg
     3901 ggtggagtag gtgctgccgc ggtcgactgt ggtcactccg gaaacgcctc ctaggccgcg
     3961 acgggccggt cgatcttcgt ctctgcggcg atgccgccca cacagaactg cacgacgttc
     4021 gcaacgagcg cgtcggtctg acggtggtcc tcgggcgccg gtgacagcgg ccccacgagc
     4081 gcctcagcga gggcgcccac gagcgcggcc gccaccacgc cgctgtcgtg cgggcgaagc
     4141 tcgcgcgact cgactccctc ggcgagcgcc gccgcgaaca cgtcgcggta ggcgtgccgg
     4201 tagcgcagcc gctccgcctc gacggccggg tcgacgggct cggcaatgag cgcgtacgcc
     4261 agcaccggcc cggcgagcgc gcgccgcgag aacgcctcga ttgcgcgcgc gatccgctct
     4321 agcaccgggc cctcgccgcc tgtcacctcc gcgacgacgt ccacctctcg ctcagtggcg
     4381 cgccggaaca cttccgcgaa gaggtctgcc ttcgagggga agtgcctgta gacggtgccg
     4441 gtcgccacgc ccgcgcgggc cgcgacggcc tgaacgctcg ccgacgcgta gccgccttcg
     4501 gccacccggt cgagcgccgc ggcgactatg cgctcgcgga ccgccgcctg gcgggcgcgg
     4561 gtcttctcgg tgtggcggta cgccatctac ccaagaagtg aatcacgatt cactccttct
     4621 gacaacgggc cccgtgccga acgagattga tattgagcac ctatacggtg ctcaagttaa
     4681 aggtcgttcc gcagaccttt tggtcgcggg ggttggagga ggaaccggcg cacgccggag
     4741 ccaccgccca gccacctgac ctttactgct tattcgaagt tcatgccgcg ctagctgacc
     4801 ggctcaggcc gcccgcgcag gatgcagtcg atcgtcgacg ccgcgctcgt cgtcgacccg
     4861 tccgcgtagc cgaacgtcga gccgaatcgc cagccgccgc gcggacagct cgtgggcgcc
     4921 tgcaagagcg acacgcgctt gccccgcagc ttcccgaacg ccttcacgtt cacgtcgagt
     4981 ttcgagatcg acgcgttcgg attgtgcggc agggtgggga tgtccgggaa ctgcatgctc
     5041 agcttgtagc cgaagcgccc gttcaccttc tccagcgtcg catcgatcac caggttgatc
     5101 tcgaagtgct ccgccatgcc gaggaagatg aagtgcggct tgccgccctt cggcttgccg
     5161 ttgaacatcg tgatcgtcgc gcgctcggga ttgatgaacg gccgcgcgtc cacgagcgcc
     5221 tcgcccgagc cgagccgcga cgcgcgcgga cacgtcctga ccgttggctc cttctgcagc
     5281 ttcaccgcgt cgcacgtggg gaacgccttg gtgttcacgg tcgcgtgcgg gaacaggaac
     5341 accgctttct tcagtggcac cggcttgctc ccgtccggcg tgccgatgcc cgccacgatg
     5401 ttcagcccga tgtcccaggg cccaccgggc gactgcttga ggagctcgac gcccgtgtct
     5461 agcgtctgcg tattcgaggc atgcgcctgc gaggcgagcg cggccagtgc gctcgccgcg
     5521 agcagcagcg cgcggaacgt cctgcgagtc caaggcaagg cggcggcatc ctaagcgcga
     5581 gaggcagatt tgcgcaaggt gtcctaacct gtccgcacca tgcgcccggc tggactcgca
     5641 atcctgggcg cgctgctcct ggccctcccc gccacggctg ctgccgcgcc gcccgatacc
     5701 cggatcactg ccgggcccga caagtacacg acgagcaaga ctccgcagtt cgcgttcgag
     5761 tcgacggttc cgggctcgac gttcgagtgc cgcgtgaact acgccggctg ggctccgtgc
     5821 acctcgccgt tcactgtcga tgcgcgctac atcgccacgt acgtgttcga ggcgcgcgcg
     5881 atcggccccg acggagaccc ggacccgacc gcggcgttcc gtcgcttcca ccgcggcgac
     5941 atcttcccgc cggtcgtgaa gctcacgcgc ttccacagca agatgaagat caagagcttc
     6001 aagcgcctga gcggtaccgc ggtcggcaca ctcggcatcg gctccgttca ggtttcgatg
     6061 cgcgacgcgg acaagcggcg cctgctgcct caccaggaga ccgagcagtg catctcggtc
     6121 gacctcgaga cgggccagcg cagctggcgg tattgcagct tcccgcagta cgtacgtgca
     6181 cgtggcggcg cgaagtgggc gttgccgatc tcgcggaagg tgcgtgagca cctcaagccg
     6241 ggccgctacg agatatgggt gcgcgcgacg aacgtgctcg gcaacggcga ggtcacgaag
     6301 ttcttcgtga cactcaagcg ctgacccatc ccgtgccccg cggcgcgtaa aggtacgcgc
     6361 ggcagaccct cacgcggcag cgcgccgtcc gaacacaccg gccgttggcg agtagcggcg
     6421 attcctcctg aaaatccctc ggaaggggca cagagatgcc tcccgcgggg gaagcccagt
     6481 ccgcatctac cggcttaggg tcgccatgcg tccctggccc gcgcccgtcg tggcccacgg
     6541 gcgtgtgcaa atcccgaaat ctccaggagg gagggactgg tgtcaaaacc ccgcagcctt
     6601 ctccggctac tgggcgtcat ggttctcgcg attgccgtag gcatcacatt cgcgggtccc
     6661 gcaagcgcca agcagtcgaa ggctcagaag cgggcgagca aggttctcat caagcagctc
     6721 aagaagaacc ctcgtctgat caagtcgaag cgctggcttc gcaaggccgc ccacgtacag
     6781 gcgactctgc cgctgacggt tcgtctgaac ccgatcatca acaagagcgc gacccccggc
     6841 gacaccaccg aggccgcttc cgatgacacc gccaacatcg acctgacgag cacgttcggt
     6901 ccgaacgttg gcaacaaggt gaccaagctg agcgggaagc tcgacgtgct tgccaccttc
     6961 gggaaccccg cggaaggcga tgacctcggt gatctgaggc tctcagtcac gtccgcgagc
     7021 ctgtccgcct cgagcgtggc cgttctggac acgcctggga cgggcgccgc gtgtgccgcg
     7081 gacaacggcg gtgccggcgc tgctgcacgc ccggacaaca ccctgaccac tggtggtctt
     7141 ggtgaccggt cgaccctcgg aaacttcgtg tctgcggctt cgcaggctcc gagcgtcacc
     7201 ccggacacga ccgttcgcac cgctccgatc tcgatcaacc tggcaagcgt tgccgggtcg
     7261 cccgcgatcg gcaaggcgaa catcttctcg gacacgaaca acgtccgcct gtcgctccac
     7321 gcggcagcga aggtcaacac gatcttccgt tccacggaga ccggtatggg cttcgcggcc
     7381 gggcagctgc cgggcgctgc gttcttctgt gacgaggcct acgccggcca gacggactcg
     7441 tccggccttg gcacgcctcc gggctctccg accaccggtg gcgacatcgg cccggtcggt
     7501 caccggaaca tcatcccggt caacgtgacc ggcaacctca agatcaaccc ggccatcacg
     7561 accgacggca agctgcgtct cgccacggtc acggtgcagt cgacgcgtcc tgaggagtca
     7621 acgatcgacg cctgcctgca gatcaactcg ttgatccgca cggcagactc gtcggcagct
     7681 tcgaacaccc tgctgggctc gccctcaggc ttcgcgctgc tgacgcgcaa cgccacgccg
     7741 accaccgctt gtgactcggc gtacacgccg tacaaccccg cgacggctac tggcacggag
     7801 ccctggcccc ttgccgccgt catcgatgcg ggaggcgaca cgctggccga cgagatcacg
     7861 ccggcctccg cgaagttcct ccagctcgac ccgaacgttg aggtcactgg cctcagcgcg
     7921 gaggcgctgg tgggcacggg cgccagctac tagcaggcgg ccgttcaccg aaggacatac
     7981 aggggccgcg cgcaagtgcg gcccctgtac ttctttaggg tgaaggcgtg gcgacccatt
     8041 aggggccgtg ttagtctggc gccggcccgg aaccggactc accgggccag ccccggactc
     8101 gcgttctaca agaggagctt cctggcccat gcggtttctc cgtgagcgtc acgcacgctt
     8161 cgtccttgcc gtcgatctgc gcccggctgc gcgcatcgcg ctggtcgcgg ctgcgacggc
     8221 gctcgcgctc atggcggtcg gcgccggacg cgccgccgcc tccacgagcg cagccgaatc
     8281 ccaggcgcgt gccctcgtgg ctcaaaacgc cgcgcacggc cagcgcgctc agcagtcggc
     8341 caagaagaag aaaaagaaga agaagctgtg cgtggtcaag aagctcgtca acggcaagct
     8401 gcgcacggtc tacatccgca cgtacgcgta ccgctacaag aagaagggcg gcaagaaggt
     8461 gcgcaccatc gtgcgcgtca agaaggcgat gcgcgcggcg tgcaccaagc actgtgtgaa
     8521 gcaggtgacg aagaacggca aggtgcggac cgtttacgtc acgcgcaagg tcaccgtgct
     8581 cgtgaagaag ggcaacaagc tcgtcaagcg caagcgcaag atgaggatgc ccgcgctcgc
     8641 gaagtgcccg tcgacgtccg gcgagacggt ggtcggcacg ccgatcaaga tcaagctcat
     8701 ccctggtaac cccgacacgg gcagcggcag cttcgcaagg ctcgacttcg gatcgttcca
     8761 gcgcgacgcg gcgctcagcg gcgagatcac aggcttcagc ccggggcgga tcgacatctc
     8821 gaaaccggtg gacttcacgc tcactggagg cgcgatcaac ctggcgccaa ccgggatctt
     8881 catcgacgat gaatgcttcg gggaagtgag ctctgccatt cgcaccgggc ctggaacgaa
     8941 catcacgctg aacaaggcga gggagaactc gggcaacctc gacccggacg cgaacgtggt
     9001 gacatcggtg atcaacgtga agatccgggc gtcgctcgag ctgcgaaacg acgtggacgg
     9061 ttgccacaag ccgtacctcc cgacgggcta caccgacacg aatgcgcgct ttctcctgcg
     9121 cggcgcactc gacagttccg gtggcctgcg cgtgcggctg gcatccacga gcgtcctgct
     9181 ggaggacttc accgcctgcc tcgcgctcgg cccggcaaac ctgccgtgca acggctttgc
     9241 ggcaccgctg ccgttcctcg tctcgaacaa ggtgctggcg tcgctcgagt tcgggaacta
     9301 cgggaagatc aaggtctcgt gaggtggagg gcgacgctgc tcgccgccgg cgcggcggcc
     9361 gtcgccctga cctcgcaggc cgctgctggg gagagggcag tggtgtcgca gggcggcgag
     9421 ccgcggctgc tggtcgcgct gacaccgggt gtggcgccgt cgcgcgtgcc cggccttcgc
     9481 gatgggcggc tggtgtcgcg cgcgctgaac gcctggaccg ctacggcggg cggctctgct
     9541 gacccggcag cgctcgcacg aagcgtcgct cgccgacgtg gcgtggttgc ggcacagccc
     9601 gacacgctca tccgccgcgc gcaggccggc atcacgtgca gcggcgcacc gggcacgccg
     9661 gacggctacc tgatcggcgc ggtgcgcgcc ggcggcgcgc cactcgacgc cacccggccg
     9721 gtggcggtcg tcgacagcgg catcgatggc tcgctcgacg aattcgcggg caaggtcgtg
     9781 aagccggtga acgtcgtgag cggcggcgcg gatgtgacgg acggagacgg ccacggcacg
     9841 ggcgtggcgg gcgtggccgc cgcgcgcgcc ggcggcatgc gcggcgttgc gcaggggagc
     9901 ccggtgatgc ccgtgaagat gttcggcgcg gacggg
//
LOCUS       DASVXS010000117        14177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_314193, whole genome shotgun sequence.
ACCESSION   DASVXS010000117 DASVXS010000000
VERSION     DASVXS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314193"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VF752_06465"
     CDS             complement(<1..787)
                     /locus_tag="VF752_06465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8855227.1"
                     /translation="MTRSRGRDSGLLLALVLAAAAIRFATLSVQSFWLDEAVTARLVR
                     HGFFDMLSKIPGSESTPPLYYALLWPWSRVFGEWEFGLRSLSALAGTALVPVVCLIGR
                     DLISRRAGLIAAALTAVNPLLVWYSQEARAYMLLALLASASFLLFERALRSGGRGALA
                     GWAMVSAAALVTHYFALFVVVPEALWLLLRGRRRRGAVMAVGAVALAGAAVLPLAIDQ
                     SDASRAHFITGTSLASRVVQVPKQYLIGYDAPAEVALAAVAGAL"
     gene            complement(784..1578)
                     /locus_tag="VF752_06470"
     CDS             complement(784..1578)
                     /locus_tag="VF752_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8855228.1"
                     /translation="MERRRLGRGGPELPVVGLGTWRTFDLPPGREEIARSVFEAMYAE
                     GSRMVDSSPMYGRAEDVLGETIAPHRDEVFVATKTWSPSQAGAEARFTQQLRFFGRHI
                     ELMQVHNLVAWRERLAWLERERDAGFVSLLGATHYSASAFSELEQVMRSGRIDAVQVP
                     LNPLERESEQRILPLAEELGLGVIAMRPFGERSLPTGPAPEEIQRELGVDTWAEALLR
                     WALSDRRVTVAIPATADPAHARSNALAGDGPWLDHDQRRLVESHVP"
     gene            complement(1578..1943)
                     /locus_tag="VF752_06475"
     CDS             complement(1578..1943)
                     /locus_tag="VF752_06475"
                     /inference="COORDINATES: protein motif:HMM:NF040913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SaoD/DsrE family protein"
                     /protein_id="HEX8855229.1"
                     /translation="MKVAYVFSTSGHTASYKLGKMILPQLEAGDHGVDVVGMMFFDDN
                     TFVLRKGDPLGERLGKVARDEGMLLMLCDQCALERGLAEGEPPSCTPKDTVDGVQVGC
                     FPDLYGALDGNPPDQVITL"
     gene            2117..3535
                     /locus_tag="VF752_06480"
     CDS             2117..3535
                     /locus_tag="VF752_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855230.1"
                     /translation="MAQAHSPEDGAPNKLARWIAAAAVLVAVVVVGVLMFSGGSSYEV
                     TARFVNASQLVKGNQVRVAGVTVGSVTGLKLAPNGLADVKLKIDGDHAPLEQGTRAII
                     RQASLSGIANRYVDLQMPQGQTGGDIPDGATIPTDRTEAAVDIDQLFRTFNAPTRKSL
                     QRFFVGSAEQFKGTEKQANEGLHYLNPALDQSRILFDKLTADTVLLNRFVIDSAKLVT
                     TAASRRADLAQLVGNLSDTTRAIGQDKAALTEVLDRTPNFMRRANTTFVNLRATLDQV
                     DPLVADAKPVAKKLQPFLPQLRAFANGARPTVRDLSRTVRNKGPKNDLVELFRSFPPL
                     ADIAVDTAVRNGASRPGAFPQTADALRKAAPLIGFFRPYTPDLMGWFDDFSHSGAYDA
                     LGGFSRSQIFFNAFSVTTGGVPVLQSLEARPSDFLATARTSQVKRCPGGADVPAPDRS
                     NVFSAAEQSALDCRESDRAVKP"
     gene            3532..5121
                     /locus_tag="VF752_06485"
     CDS             3532..5121
                     /locus_tag="VF752_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855231.1"
                     /translation="MTRVLVATLAVVAACLAIVFGTGATSDSGTGKHWKLEFDNAFGL
                     TNGTDLKVAGVRAGKVTNLQLSKGFPPKAIVSVEATQKGLRSLRTDATCEIRPQSLIG
                     EYFVDCNPGASSKEIPDGGTLPVTHTTSTIPLDVVQNILRKPYSERLRIIILTLGAGL
                     AGRPQDLSATIHRAAPGLRATTQTLGILARQEKILDRFIRDSDTVIAALARRKTDVRD
                     FVHQAARTASVAATRRDALAQSMTLLPPFLAELRPTMARLGELADAQIPVLRNIRRAG
                     PDLTTAFERLGPFADAARPGLRSLGDLSRTGSSAIRASDPVVAQLREVSDQAPELAKP
                     LRQFLQTIDDRKRSIETDTRARATAPPAPDKTAYRSGQGFTGMESLLNYAYWQTLAIN
                     GFDQVSHVLRFTILVSGCSPYQTNPSADTIKNCTSWLGPHQPGVNAPDPTAKGGAAKT
                     VDVSARDRRTSSRGGDTGGNAPSTPPKPALPPELQSLLGGLTGKGAPSLPSAPSAPAP
                     QSGRGATNDAANSVLDYLLGP"
     gene            5118..6659
                     /locus_tag="VF752_06490"
     CDS             5118..6659
                     /locus_tag="VF752_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855232.1"
                     /translation="MSNKRASIVASPVLIGGVTVLIVIVAVFLAYNSNQGLPFVPTYD
                     INVELPNGAKLVKGNSVRIGGNNVGQISELRPVTITQDGQPVTVALAKLKLKKSVQPL
                     PKDTIVTVRDRSALGLKYLDLIPGNASEGYKAGATVPLANARARVDLEDVLSTFDEET
                     RVATQQALVGYGDAFTARGADLNVTIGELPSLTLHLRNVMENLSAPSTQLNQLFKQIG
                     AAAAQVAPIANVQVDLLVNLRDTLAALSRDPRALQDTIAKTPGTLQTGIESFRVQQPF
                     LAHFTALSRDLEPATAALPSALPPLSRALAAGVPTLSRSVPRLSSDTKQLFTSLADLS
                     RDPATLVSLRQLETTVNVGAPAVQFLAPYQTVCNYFNYFFDPLGEHLSEPVERGTIER
                     IGFMTANMTQDNSLAAGNADRPADVPANEDPHTAQDAGGALTKLNTQFGGPAIDKNGN
                     ADCQAGQTGYPVGPLNAGGRYPPSNDPAQGGGSHIVVDPNTPGNAGGTYVTRKLGIKN
                     LKDVP"
     gene            6656..8170
                     /locus_tag="VF752_06495"
     CDS             6656..8170
                     /locus_tag="VF752_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855233.1"
                     /translation="MSRLRPFKGREVSSFAAGAIALVLIAVVVFFVVTRANPFSNPYK
                     MSALFTNVENLQRGSEVRTAGVVIGRVKSIEAVEDGSGVALVNMEINDNGLPIHRDAQ
                     LQIRPRIFLEGNYFVDVKPGSPSAPTVKSGSTLPIQQTASSVSFGDILKVLDSNTRAD
                     LQILLREYSKALDKGGAEAFRQSTKFWPAAYRNTAIASEALLGEDPSTDIPQLLRGQQ
                     RTFAALVRSPRNLADLITNLNRTARAFASEDQALAASIPALRDVLRVGYPTLATVNNA
                     LPSVARFARDARAPVRSIGPAVDAATPFIAQLARLVSEDELRGASRQLRATVPPLVSL
                     NQDLEPFLSQGRLLSSCTSNVLVPWATTPIPDPNFPDSNNQPFYKEAPRALVGLSGES
                     RTGDANGQWFRVLVGGGPNTTVERPPSGLPLFAQTPFPSLGTQPTVPTSKPQFRPDVP
                     CETQQPADMSADTGPGDPLSSGGPGSGLLPLSPAQKRDIEALREALAKDLGGGK"
     gene            8167..9492
                     /locus_tag="VF752_06500"
     CDS             8167..9492
                     /locus_tag="VF752_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855234.1"
                     /translation="MRLAIRKYWGSFLTLIGLIVVACAIGVYILNKQGLRLPWEATPF
                     TMKAELSEAEGVKPGQHQAVRIAFVSVGQITKTELQNGHAVLTLEIQPKYRNTIRRDA
                     TALLRPKTGLEDMFLDIDPGTASSPPVKSGFTIPVSNTLPPVQSEEILNTLDADARAY
                     LQLLVNGGGEGLQGRGDDLREVFKRLLPLHRDLAKVSGVVARQDRKVRRLIHNYGELT
                     SELAKGDRDLTTVVGASNQVFSAFAQHEGDVRRWVAELPGALRQTESTLGKVNTFATE
                     LPPALDALTPALQALERANPSVTRLAKEGTPIVRNQIRPFAIRLQPWLTNLRPAATSL
                     SAATPDLTQATRELNRFFNMAAFNPGGAQRANGDPTRNEGYLFWLGWLVHNTNSLFST
                     SDASGPFRRATFAISCPTIRTVVAFNPAAVQVLGLTGLLSNECATGAGG"
     gene            9531..10949
                     /locus_tag="VF752_06505"
     CDS             9531..10949
                     /locus_tag="VF752_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8855235.1"
                     /translation="MIVFALSCVLILLFLWISFGGSTPLAAKSYRVKVAFPEAQLLVT
                     EADVRISGINVGKVKTLTVQKSPPRTVATLEIDHQYGPIPKDTKATLRQKTLLGETYV
                     ELTPGDRSSGNVADGGRLADGNVAGAVTLDDIYQAFDPPTRQAIRGWIGENAKVIDGR
                     SVDVNAVLGNLPGFASSGNKLLGVLDARGRALHNFVRNTGVVFAALNQRDQGLRDLIL
                     NSNRLFQTTAERDNALAEIIQVFPTFLDETKATLARLETFSRTTDPLVQDLRPVARDL
                     GPTVQDIGALSPDLTNLFHDIDFLSGVAPTTLPDAARVLRGARPALQSLAQFLPELNP
                     IVSFGNFHASTIADFATVGAAALGGTIPGTNPPNHYLRQLGIINDRSLALNLSRPAYE
                     RGNAYLAPNAWQRFRPLGVLESFDCAPAGGVKREPTPTQPPCYVAPPNLWDGKMFPRL
                     ARGRAPLVPPPLGNEGTRPARP"
     gene            complement(10966..11442)
                     /locus_tag="VF752_06510"
     CDS             complement(10966..11442)
                     /locus_tag="VF752_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8855236.1"
                     /translation="MALENIPSDWRLHGVRVVHADELDPNTPQTPGMHREAAINAARA
                     GAEKLWAGTVVIHPDAKTGPHHHGELESVIYVVSGRARMRWGERLEFTAEAAQGDFIY
                     VPPYVPHQEINANADESLSCVVVRSGQEPVVVNLDLPAVEPNPEQVHWVDDIHREP"
     gene            complement(11451..12857)
                     /locus_tag="VF752_06515"
     CDS             complement(11451..12857)
                     /locus_tag="VF752_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8855237.1"
                     /translation="MGTIAASTIKLDPSALKDLEGSFRGELVRPGDPSYDEHRKVWNG
                     SIDRQPALIARCTGVADVIAAVKFCRRIGLPVAVRSGGHSFPGHSVCDEGIVIDLRPM
                     QGIVVDPVARTARAQAGVLWGELDRETQQFGLATVGGIVTHTGVAGLTLGGGIGWLQR
                     KFGLTIDQLLSVSLVTADGELVRASRGENADLFWGVRGAGGNFGIVTDFEFRLHEVGP
                     TVLAGPIFWRMQDAPELLRFYREWIADAPDELMTIVVNRKAPPLPFIPAEMHGELVTA
                     VACCYAGPIEDGEQAVSALRKFGSPVLDACQPKPYVAHQAMFDPSFPHGWHYYMRACD
                     VDQLSDEVIDITVEHSLKIESPLTGFPIWQLGGAVSRVDEDDSAFGSRSAVHTFNITG
                     MTATAEGFEHEREWSRNFWTALSPHHTSVYVNFLMEEGEDRIRQAYGPKRYERLKALK
                     RHWDPDNFFRLNQNITPG"
     gene            complement(12906..13910)
                     /locus_tag="VF752_06520"
     CDS             complement(12906..13910)
                     /locus_tag="VF752_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855238.1"
                     /translation="MARTAQSPRRRLPVVAVAVIAGVAASAPSSEATFHGKPGPIAFQ
                     RLIDPRNGESAQIFSLARLGAKPHQLTSGGEALNADYSPDGQQILFERRFGGMQPDAI
                     FTMGSDGSSPTRFRSTCTPDACLGDSAPAWSPDGNRLVFERAFGPIVKDNAAGVDIVT
                     ANADGSNEQVILHYRTREAQRQEPHDAQWSPDGKRIAVTILNTTVKPRNASAIYVVDA
                     DGSNLHRVTPLRLNAGNPDWSPDGKRIAFNSSFAGQGAVELYTARPDGSGLRRLRREP
                     KRSYSFDPGWSPDGRRLAFVHGAGRGIPHIWTIRKNGTGLVQVTRGRRPDFGPDWGSR
                     "
     gene            complement(13949..>14177)
                     /locus_tag="VF752_06525"
     CDS             complement(13949..>14177)
                     /locus_tag="VF752_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855239.1"
                     /translation="PSAPQARDDAGRACGRARFHACAFNGSGARAHAVGRSGTGSHRR
                     AAGAGKRRIRPLTLRTRGARTNALHARDAPG"
BASE COUNT         2261 a         5058 c         4791 g         2067 t
ORIGIN      
        1 cgagcgcccc ggcgacggcc gcgagcgcga cctcggcggg agcgtcgtag ccgatcaggt
       61 actgcttcgg cacctgcacc acccggctcg cgagcgaagt gccggtgatg aagtgcgcgc
      121 gcgacgcgtc gctctggtcg atcgcgagcg gcagcacggc ggcgcccgcc agcgcgaccg
      181 caccgaccgc catcacagcg ccacggcggc gccgaccacg cagtagcagc cagagcgcct
      241 ccggcactac cacgaacagc gcgaagtagt gagtcacgag cgcggccgcc gacaccatcg
      301 cccagcccgc cagcgcaccg cgaccgccgc ttcgaagcgc gcgctcgaac agcaggaacg
      361 acgcactcgc cagcagcgcg agcagcatgt atgcgcgcgc ctcctgcgag taccagacga
      421 gcagcgggtt gacggccgtg agcgcggcgg cgatcaggcc tgcccgccgt gagatcaggt
      481 cgcgcccgat caggcacacg accggcacga gcgcggtgcc ggccaacgcc gacagcgagc
      541 gcagcccgaa ctcccactca ccgaacaccc gcgaccaagg ccacagcagc gcgtagtaga
      601 gcggcggtgt ggactcgctg ccggggatct tcgagagcat gtcgaagaag ccgtggcgca
      661 cgagccgcgc ggtgaccgcc tcgtcgagcc agaagctctg gaccgacagg gtggcgaacc
      721 ggatcgcggc cgccgcgagc acgagcgcta ggagcaggcc ggagtcgcgg ccgcggctac
      781 gggtcacggc acgtggctct ccaccagccg gcgctggtcg tggtcgagcc aagggccgtc
      841 gccggccaag gcgttcgaac gagcgtgcgc cgggtccgcc gtcgccggta tggcgaccgt
      901 cactcggcga tccgataggg cccagcgcaa cagcgcctcg gcccacgtgt caacgccgag
      961 ctcgcgctgg atctcctcag gcgccgggcc ggtcgggagc gagcgctcgc cgaaggggcg
     1021 catcgcgatg acgccgagcc cgagttcctc ggcgagcggc aggatgcgct gctcgctctc
     1081 tcgctcgagc gggttgagcg gcacctgcac cgcgtcgatg cgaccgcttc gcatgacctg
     1141 ctcgagctcg gagaacgcgg aggccgagta gtgcgtcgcg ccaagcagac tcacgaatcc
     1201 ggcgtcgcgc tcgcgctcga gccaggcgag ccgctcgcgc cacgcgacga ggttgtgcac
     1261 ctgcatgagc tcgatgtggc ggccaaagaa gcgcagctgc tgagtgaagc gtgcctccgc
     1321 gccggcctgc gacggcgacc acgtcttggt ggcgacgaag acctcgtcgc gatgcggcgc
     1381 gatcgtctcg ccaagcacgt cctcggcacg tccgtacatg ggcgaggagt ccaccatgcg
     1441 gctgccctcg gcgtacatcg cctcgaacac cgaccgggcg atctcttcgc ggcctggcgg
     1501 caggtcgaac gtgcgccacg tgcccagtcc gacgaccggc agctccggac cgccgcgtcc
     1561 gaggcgcctg cgctccacta gagggtgatg acctggtccg gcgggttccc gtcaagggcg
     1621 ccgtacaggt cgggaaagca tccgacctgg acgccgtcga cggtgtcctt cggcgtgcag
     1681 ctcggcggct cgccctcagc cagcccccgc tcgagcgcgc actggtcgca gagcatgagc
     1741 aacatgccct cgtcgcgcgc gaccttgccc aggcgctcgc ccagcgggtc gccctttcgt
     1801 agcacgaacg tgttgtcgtc gaagaacatc attccgacga cgtcgacgcc gtggtcgccg
     1861 gcctcgagct gcggcaggat catcttcccg agcttgtagc tggccgtgtg accagacgtt
     1921 gagaacacgt aggcgacctt catgactccc ttccgtgact tgtgctgagc gctgagtctg
     1981 acgacttcgg ttcggcggaa gtgggtccgt cgcccaacag ttcttaatca gtggtggttt
     2041 tcgaccctgc ctgttgcggg aatgtgaacc agggtgcgcg ggggaggacg cgggaaccgc
     2101 cacggagagg cagtaattgg cccaagccca tagccccgaa gacggggcgc cgaacaagct
     2161 cgcaaggtgg atcgccgccg ccgcggtgtt ggtcgcggtg gtggttgtgg gcgtgctcat
     2221 gttctccggc ggctcctcgt atgaggtcac cgcgcgcttt gtcaacgcct ctcaactcgt
     2281 aaagggcaac caggtccgcg tcgcgggcgt gacggtcggc agcgtcaccg ggctgaagct
     2341 cgccccgaac ggcctggctg acgtgaagct gaagatcgac ggggaccacg cgccgctcga
     2401 gcagggcacc cgcgcgatca tccgccaggc gtcgctctcc ggcatcgcga accgctacgt
     2461 ggatctccag atgccgcagg ggcagacggg cggcgatatc cccgacggcg ccacgatccc
     2521 gacggaccgc acggaggccg ccgtcgacat cgaccagctg ttccgcacgt tcaacgctcc
     2581 cacgcgcaag tcgctgcagc gattcttcgt gggctcggcg gagcagttca agggcaccga
     2641 gaagcaggcc aacgagggcc tgcactacct gaaccccgcg ctcgaccaga gccgcattct
     2701 gttcgacaag ctcacggcgg acacggtgct gctgaaccgc ttcgtgatcg acagcgcgaa
     2761 gctcgtgacc accgccgcat cgcggcgcgc cgacctcgcc cagctcgtcg gaaacctgag
     2821 cgacacgacg cgcgctatcg gacaggacaa ggcggcgctc accgaggtgc tcgaccgcac
     2881 gccgaatttc atgcgccgcg cgaacacgac gttcgtgaac ctgcgcgcca cgctcgacca
     2941 ggtcgatccg ctcgtggccg acgccaagcc ggttgcgaag aagctccagc cattcctgcc
     3001 gcagctgcgg gcgttcgcga acggcgcccg cccgaccgtc cgcgacctct cgcggacggt
     3061 gcgcaacaag ggcccgaaga acgacctcgt ggagctgttc cgcagcttcc cgccgctcgc
     3121 cgacatcgcg gtcgacacgg ctgtgcgcaa cggcgcgagc cggccgggcg cgttcccgca
     3181 gaccgccgat gcgctcagga aggcggctcc cctgatcggc ttcttccggc cgtacacgcc
     3241 ggacctgatg ggctggttcg acgacttctc acactcgggt gcctatgacg cactcggcgg
     3301 cttctcgcgg tcgcagatct tcttcaacgc cttcagcgtc acgacgggcg gcgtcccggt
     3361 gctgcagagc ctcgaggcgc ggccgagcga tttcctcgcg accgccagaa cctcacaggt
     3421 caagcgctgc ccgggtggcg ccgacgtgcc cgcccccgac cgctcgaacg tgttctcggc
     3481 ggccgagcag agcgcgctcg actgccgcga gagcgacagg gcggtgaagc catgacccgt
     3541 gtgctcgtgg cgacgctcgc cgtcgtggcc gcctgtctgg cgatcgtctt cggtaccggc
     3601 gccacgagtg acagcggcac cggcaagcac tggaagctcg agttcgacaa cgcgttcgga
     3661 ctgacgaacg gcaccgacct caaggtcgcc ggcgtgcggg ccggcaaggt cacgaacctc
     3721 cagctctcca agggcttccc gccgaaagcg atcgtgagcg tggaggcgac ccagaagggg
     3781 ctgcgctcgc tccgcacgga cgccacctgc gagatccgcc cgcagtcgct gatcggcgag
     3841 tacttcgtgg actgcaaccc gggcgcgtcg agcaaggaga tacccgacgg cggcacgctg
     3901 ccggtgacgc acaccacgtc cacgatcccg ctcgacgtgg tgcagaacat cctgcgcaag
     3961 ccgtactccg agcgcctgcg gatcatcatc ctcacgctcg gcgccgggct cgccgggcgc
     4021 ccgcaggacc tgagcgccac catccaccgc gccgcgccgg gcctgcgcgc caccacccag
     4081 acgctcggga tcctcgcgcg gcaggagaag atcctcgacc gcttcatcag ggactccgac
     4141 acggtgatcg cggcgctcgc gcggcgcaag accgacgtgc gcgacttcgt ccatcaagcc
     4201 gcgcggaccg cgtcggtggc cgccacgcgt cgcgacgcgc tcgcgcaatc gatgacgttg
     4261 ctcccgccgt tcctcgccga gctgaggccc acgatggcgc gcctaggcga gctggcggac
     4321 gctcagatcc cggtgctgcg caacatccgc cgcgccggtc ccgacctcac gaccgctttc
     4381 gagcgcctcg gtccgttcgc ggacgccgcg cggccaggcc tgcggtcgct cggcgatctc
     4441 tcgcggaccg gctcgagtgc gatcagggcg agcgatcccg ttgtggcaca gctccgcgag
     4501 gtttccgacc aggcgcccga gctcgccaag ccgctgcgcc agttcctcca gacgatcgac
     4561 gaccgcaagc gctcgatcga gaccgacacg cgcgccaggg cgaccgcgcc gccagcgccc
     4621 gacaagacgg cctacaggtc cggtcagggc ttcacgggca tggagtcgct cctcaactac
     4681 gcctactggc agacgctggc gatcaacggc ttcgaccagg tgagccacgt gctgcgcttc
     4741 acgatcctcg tgagcgggtg ctcgccgtac cagacgaacc cgtcggcgga caccatcaag
     4801 aactgcactt cgtggctcgg gccgcaccag ccgggcgtca acgcgcctga cccgaccgcg
     4861 aagggaggcg cggcgaagac cgtcgacgta tccgctcgcg acaggcggac atcctcgcgc
     4921 ggcggcgaca cgggcggcaa cgcgccgagc acgccgccca agcccgccct cccgcccgag
     4981 ctccagtcgc tgctcggcgg gctcaccggc aagggcgcgc cgtccctgcc gtctgctccg
     5041 tccgccccgg cgccgcagtc cggccgcgga gccaccaacg acgccgccaa ctccgtcctc
     5101 gactacctgc tcgggccatg agcaacaagc gcgcgagcat agttgcgagc ccggtgctga
     5161 tcggcggggt cacggtgctg atcgtcatcg tggccgtctt cctcgcctac aactcgaacc
     5221 agggcctccc gttcgtgccc acgtacgaca tcaacgtgga gctgccgaac ggggccaagc
     5281 tcgtgaaggg caactcggtg cggatcggcg gcaacaacgt cggccagatc tccgagctgc
     5341 gcccggtcac gatcacccag gacggccagc cggtcacggt cgcgctcgcg aagctcaagt
     5401 tgaagaagtc ggtccagccg ctgccgaagg acacgatcgt tacggtccgg gaccgctcgg
     5461 cgctgggact gaagtacctc gacctcatcc ccggcaatgc gagcgagggt tacaaggcgg
     5521 gcgccaccgt gccgcttgcc aacgcgcgcg cacgggtgga cctcgaggac gtgttgtcca
     5581 ccttcgacga ggaaacgcgc gtggccacgc agcaggcgct cgtgggatac ggcgacgcct
     5641 tcacggcgcg gggcgccgac ctgaacgtga cgatcggcga gctgccgtcg ctgacactcc
     5701 atctaaggaa cgtgatggag aacctctcgg cgccgagcac ccagctcaac cagctcttca
     5761 agcagatcgg cgcggcggcg gctcaggtgg cgccgatcgc gaacgtccag gtcgacctgc
     5821 tcgtgaacct ccgcgacacg ctggccgcgc tgtcgcgcga cccgcgcgcg ctgcaggaca
     5881 cgatcgcgaa gacacccggc acgctgcaga ccggcatcga gagcttccgc gtgcagcagc
     5941 cgttcctcgc gcacttcacg gcgctctcgc gcgacctcga accggccacg gccgccctgc
     6001 caagcgcact gccgccactc tcgcgcgcgc tcgccgcagg cgtgcccacg ctcagccgga
     6061 gcgtgccgcg cctcagcagc gacaccaagc agctcttcac ttcgctcgcg gacctctcgc
     6121 gcgacccggc cacgctcgtc tcgctccgcc agctcgagac cacggtgaac gtgggcgcgc
     6181 cggcggtgca gttcctggct ccgtaccaga cggtttgcaa ctacttcaac tacttcttcg
     6241 atccgctcgg cgaacacctc tccgagccgg tcgagcgcgg cacgatcgag cggatcgggt
     6301 tcatgaccgc gaacatgacg caggacaact cgctcgcggc cggcaacgcc gatcgcccag
     6361 cggacgtgcc cgcgaacgaa gacccgcaca cggcgcagga cgccggtgga gcgctcacca
     6421 agctgaacac gcagttcggc ggcccggcga tcgacaagaa cggcaacgcc gactgccagg
     6481 cgggtcagac cggctatccg gtcgggccgc tcaacgccgg cgggcgctat ccgccgagta
     6541 acgaccccgc gcagggcggc ggcagccaca tcgtcgtgga cccgaacacg ccgggcaacg
     6601 ccggaggcac ctacgtgact cgcaagctcg gcatcaagaa cctgaaggac gtgccatgag
     6661 ccggctgcgc ccattcaagg gacgcgaggt ctcgtccttc gccgccggcg ctatcgcgct
     6721 cgtgctcatc gccgtcgtgg tcttcttcgt cgtgacccgc gcgaacccgt tctcgaaccc
     6781 ctacaagatg tcggcgttgt tcacgaacgt cgagaacctc cagcggggtt ccgaggtgcg
     6841 tacggcgggc gtcgtgatcg gaagggtcaa gagcatcgag gccgtggagg acggctcggg
     6901 cgtcgctctc gtgaacatgg agatcaacga caacggcctg ccgatccacc gcgacgcgca
     6961 gctgcaaatc cgtccgcgca tcttcctcga gggcaactac ttcgtggacg tgaagccggg
     7021 ctcgccgtcg gccccgaccg tgaagtccgg cagcacgctg ccgatccagc agacggccag
     7081 ctcggtgagc ttcggcgaca tcctaaaggt gctcgactcg aacacgcgcg cggacctgca
     7141 gatcctgctg agggagtact cgaaagcgct cgacaagggc ggcgccgagg cgttcaggca
     7201 gtcgaccaag ttctggcccg ccgcgtatcg caacacggcg atcgcgagcg aagcgctgct
     7261 cggtgaggac ccgagcacgg acatcccgca gctgctgcgc ggacagcagc gcacgttcgc
     7321 cgcgctcgtg cggagcccac ggaacctggc ggacctgatc acaaacctga accgcaccgc
     7381 tcgcgcgttc gcttcagagg atcaggctct agccgcgtcg atacccgcgc tgcgcgatgt
     7441 gctgcgcgtc ggctacccga cactcgccac ggtgaacaac gcgctgccgt cggtcgctcg
     7501 cttcgcgcgc gacgcgcgcg cgccggtgcg ctcgatcggc cccgccgtag acgcggccac
     7561 gcccttcatc gcgcagctgg cgcggctggt gtccgaggac gagctccgag gcgcctcgcg
     7621 ccagctgcgc gcgaccgtgc cgcccctcgt gtcgctgaac caggacctcg agccgttcct
     7681 gtcgcagggg cggctgctca gctcctgcac ttcgaacgtg ctcgtgccgt gggccaccac
     7741 gccgatcccc gatccgaact tcccggacag caacaatcag cccttctaca aggaggcgcc
     7801 gcgcgcgctc gttgggctct ccggcgagag ccgcaccggc gacgcgaacg gccagtggtt
     7861 ccgcgtgctc gtgggcggtg ggccgaacac gaccgtcgag cgcccgccga gcggtctgcc
     7921 gctgttcgcg cagaccccgt tcccgtcgct cggcacgcag ccgacggtgc ccacctcgaa
     7981 gccgcagttc cggcccgacg tgccgtgtga gacgcagcag ccggccgaca tgtcggcgga
     8041 caccggcccc ggtgaccccc tgagcagtgg cggaccgggg agcgggctcc tcccgctcag
     8101 tccggcccag aagcgcgaca tcgaggcgct gagggaggca ctcgccaagg acctcggagg
     8161 cggcaagtga ggctcgccat ccgtaagtac tggggcagct tcctcacgct gatcgggctg
     8221 atcgtcgtgg cgtgcgcgat cggcgtgtac atcctcaaca agcagggcct ccggctgccg
     8281 tgggaggcga cgccgttcac gatgaaggcc gagctgtccg aggccgaggg cgtgaagccg
     8341 ggacagcacc aggcggtccg gatcgcgttc gtgtcggtcg gtcagatcac gaagaccgag
     8401 cttcagaacg ggcatgccgt gctcacgctc gagattcaac ccaagtaccg caacaccatc
     8461 cggcgggacg cgaccgcgct gctgcgcccg aagaccggcc tcgaggacat gttcctcgac
     8521 atcgatccgg gcacggcgag ctcgccgccg gtgaagtccg gcttcaccat cccggtgtcg
     8581 aacacgctgc caccggtgca gtcggaggag atcctcaaca ccctcgacgc cgatgctcgc
     8641 gcttatctcc agctgctcgt gaacggcgga ggcgaggggc ttcagggccg cggcgacgac
     8701 ctccgcgagg tgttcaaacg actcttgccg ctccaccggg acctcgccaa ggtgagcggc
     8761 gtggtcgccc gccaggaccg gaaggtgcgg cggctcatcc acaactacgg cgagctcacc
     8821 agcgagctcg caaagggcga ccgcgacctg accactgtgg tgggcgcttc gaatcaggtg
     8881 ttcagcgcgt tcgcgcagca cgagggcgac gtgcggcgct gggtggccga gctgccgggc
     8941 gcgctgcgcc agaccgagtc gacgctcggc aaggtgaaca ccttcgccac cgagcttccg
     9001 cccgcgctcg acgccctgac gcctgcgctg caggcgctcg agcgggcgaa cccttcggtc
     9061 acgcggctcg cgaaggaggg gacgccgatc gtgcgaaacc agatccggcc gttcgcgatc
     9121 cgtctgcagc cgtggctgac caacctgcgc cccgcggcga cgtcgctgtc ggccgctacg
     9181 cccgacctga cgcaggccac gcgcgagctg aatcgcttct tcaacatggc tgccttcaac
     9241 ccgggcggcg ctcagcgggc taacggcgat ccgacccgga acgagggcta cctcttctgg
     9301 ctcggctggc tcgtgcacaa cacgaactcg ctcttctcga cgtctgacgc ctcagggccg
     9361 ttcaggcgcg cgaccttcgc gatcagctgc ccgacgattc ggacggtcgt cgcgttcaac
     9421 ccggccgccg tgcaggtgct gggcctgacc gggctcctca gcaacgagtg cgcaacgggg
     9481 gcgggcggct gatggtgaag caggcgccag ggcgcggtcg gatggcggcg atgatcgtct
     9541 tcgccctgtc gtgcgtgctc atcctgctgt tcctgtggat ctcgttcggc ggctccaccc
     9601 cgctcgccgc gaagagctac cgcgtgaagg tggcgttccc ggaggcgcag ctgctcgtga
     9661 ccgaggccga cgtgcgcatc tctggcatca acgtcggcaa ggtgaagacg ctgaccgtgc
     9721 agaagagccc gccgcgcacg gtcgcgacgc tcgagatcga ccatcaatac gggccgattc
     9781 cgaaggacac gaaggcgacg ctgcgacaga agacgctgct cggggagacc tacgtggagc
     9841 tcactcccgg cgaccggagc tcggggaacg tggccgacgg cggccggctc gcggacggca
     9901 acgtggcggg cgcggtaacg ctcgacgaca tctatcaggc gttcgacccg cccacgcgcc
     9961 aggcgattcg cggctggatc ggcgagaacg cgaaggtgat cgatggacgc tccgtcgacg
    10021 tgaacgccgt gctcggcaac ctgcccggct tcgcctcgtc gggcaacaag ctgctcggcg
    10081 tgctcgacgc gcgcggccgc gcgctccaca acttcgtgcg caacaccgga gtggtgttcg
    10141 cggcgctgaa ccagcgcgac cagggcctgc gcgatctgat cctcaactcg aatcgcctgt
    10201 tccagaccac tgccgagcgc gacaacgccc tcgcggagat catccaggtg ttcccgacct
    10261 tcctcgacga gacgaaggca acgctggcgc ggctcgagac gttctcgcgg accaccgatc
    10321 cactcgttca ggacctgcgg cccgtggcgc gcgacctcgg cccgaccgtg caggacatcg
    10381 gcgcgctctc gccggacctc acgaacctgt tccacgacat cgacttcctc tcgggtgtgg
    10441 cgccgacgac gctgccggac gcggcacgtg tcctccgcgg ggcgcgcccg gcgttgcagt
    10501 cgctcgcgca gttcctgccc gagctgaacc cgatcgtgtc gttcggcaac ttccacgcca
    10561 gcacgatcgc ggacttcgcc acggtcgggg ccgccgccct cggcggcacg atcccgggca
    10621 cgaacccgcc gaaccactat ctccgccagc tcggcatcat caacgaccgc tcgctagcac
    10681 tcaacctgag ccgtccggcg tacgagcgcg gtaacgcgta cctcgctccg aatgcgtggc
    10741 agcgcttccg gccgctcggc gtgctcgaga gcttcgactg cgcgccagcg ggcggggtca
    10801 agcgcgagcc gacccccacc cagccgccgt gctacgtggc gccgccgaac ctgtgggacg
    10861 ggaagatgtt cccgcgcctc gcgcgcggca gagcgccgct cgtgccgccg ccgctcggga
    10921 acgaggggac gcgcccggcc cggccctagg cgccggcgcg ggagctcaag gctcccgatg
    10981 gatgtcgtcg acccagtgga cctgctccgg attgggctcg acggccggca ggtcaaggtt
    11041 gacgacgacg ggctcctggc cgctgcgcac caccacgcag gacagggatt cgtcggcgtt
    11101 ggcgtttatc tcctggtggg ggacgtacgg cggcacgtag atgaagtcgc cctgcgcggc
    11161 ctcggccgtg aactcgagcc gctcgcccca gcgcatgcgc gcgcggccgc tgaccacgta
    11221 gatgacgctc tcgagctctc cgtggtgatg ggggccggtc ttcgcgtcag ggtggatcac
    11281 gaccgtgccg gcccagagct tctctgcgcc cgcccgtgcg gcgttgatcg ccgcttcgcg
    11341 gtgcatcccc ggggtctgtg gcgtgttcgg gtcgagctcg tccgcgtgca ccacccgaac
    11401 gccgtgcagt cgccagtcgg acgggatgtt ctcgagtgcc atggctcgag ctagccgggc
    11461 gtgatgttct ggttgaggcg gaagaagttg tcgggatccc agtgccgctt cagcgccttc
    11521 agacgctcgt accgtttcgg cccgtaggcc tgccggatcc ggtcctcgcc ctcctccatc
    11581 aggaagttga cgtagacgct cgtgtggtgt ggggacagcg ccgtccagaa gttgcgtgac
    11641 cactcgcgct cgtgctcgaa gccttcggca gtggcggtca tgccggtgat gttgaaggtg
    11701 tggaccgcgc tgcggctgcc gaacgcgctg tcgtcctcat cgacgcgaga gaccgcaccg
    11761 ccgagctgcc agatcgggaa gccggtgagc ggcgactcga tcttcagcga gtgctcgacg
    11821 gtgatgtcga tcacctcgtc gctcagttgg tccacgtcgc acgcgcgcat gtagtagtgc
    11881 cagccgtgcg ggaaggaggg gtcgaacatc gcctggtggg ccacgtacgg cttcggctgg
    11941 cacgcgtcga gcactggcga gccgaacttc cggagcgcgc tcacggcctg ctcaccgtcc
    12001 tcaatcggtc ccgcgtagca gcaggcgacc gccgtcacga gctcgccatg catctccgcg
    12061 ggaatgaacg gcagcggtgg cgccttccgg ttgaccacga tcgtcatcag ctcgtccggc
    12121 gcgtcggcga tccattcgcg gtagaagcgc aggagctcgg gcgcatcctg catgcgccag
    12181 aagatcgggc cggcgagcac ggtcggcccg acctcgtgga gacggaattc gaagtcggtg
    12241 acgatgccga agttaccgcc cgcgccgcgc acgccccaga agaggtccgc gttctcgccc
    12301 cggctcgccc tcaccagctc gccgtctgcg gtgacgaggc tcaccgacag cagctggtcg
    12361 atcgtcagcc cgaacttgcg ctggagccag ccgatgccgc cgccaagcgt gagaccggcg
    12421 acgcccgtgt gcgtgacgat cccgcccacg gtcgcgaggc cgaactgctg cgtctcgcgg
    12481 tcgagctctc cccacaagac cccggcttgg gcgcgcgcgg tgcgtgccac cggatccacg
    12541 acgatgccct gcatcggccg caggtcaatg acgatccctt cgtcgcacac cgagtgaccc
    12601 gggaagctgt gcccgccgct ccgcacagcg accggcaggc cgatccgccg gcagaacttc
    12661 accgccgcga tcacgtccgc cacaccggtg cagcgagcga tcagggcggg ctggcggtcg
    12721 atcgagccgt tccagacctt gcggtgctcg tcgtagctcg gatcgcccgg gcgaacgagt
    12781 tccccgcgga acgatccctc gaggtccttc aacgcgctcg ggtcgagctt gattgttgat
    12841 gcggcgatgg tgcccatcga gcgcgattct ccaccgccga gggtggccgc cgcaagcgcg
    12901 cctgtctacc gcgaccccca gtcgggcccg aagtcgggac gccgcccgcg ggtcacctgg
    12961 actagcccgg tcccgttttt ccttatcgtc cagatgtgtg ggatgcctcg cccagcgccg
    13021 tgaacgaacg cgaggcgtcg accgtccggc gaccagccgg gatcgaatga gtagctgcgc
    13081 ttcggctcgc ggcgcaggcg ccgcaggcca ctgccgtccg ggcgcgcggt gtagagctcg
    13141 acggcccctt ggcccgcgaa ggaggagttg aaggcgatcc gcttcccgtc cggcgaccag
    13201 tccgggttgc cggcgttgag ccggagcgga gtgacccggt ggagattcga tccgtcggcg
    13261 tcgacgacgt agatcgcaga cgcgttgcgc ggcttgaccg tggtgttgag gatcgtcacc
    13321 gcgatccgct tgccgtctgg cgaccactgc gcatcatgcg gctcctgccg ctgtgcctcg
    13381 cgggtccggt agtggaggat cacctgctcg ttgctcccgt cggcgtttgc ggtgacgatg
    13441 tccacccccg cggcgttgtc cttgacgatc ggcccgaatg cgcgctcgaa cacgagccgg
    13501 ttcccatcgg gggaccaggc gggggcgctg tcgcccaggc aggcgtccgg agtgcaggtc
    13561 gatctgaacc gcgtcggcga cgagccgtcg gagcccatgg tgaagatcgc gtccggttgc
    13621 atgccgccga agcggcgctc gaagaggatc tgctggccgt ccggtgagta atcggcgttg
    13681 agtgcctcgc ctcccgaggt cagctggtga ggtttcgctc ccagccgggc gaggctgaag
    13741 atctgcgcgc tttccccatt cctgggatcg atgaggcgct ggaaagcgat cggtcccggc
    13801 ttgccatgga aagttgcctc cgacgacggg gccgatgccg cgacgccggc aatgaccgcg
    13861 acggcgacga cagggagacg acgccgtgga ctctgggccg tgcgggccat tgttcctccg
    13921 tatggcgggg cctggttctt aggcgagcct aaccaggcgc atcccgcgcg tgcaacgcgt
    13981 tcgtgcgggc tccacgggtc cgcagagtca ggggccgaat tcgccgcttg ccggcgcctg
    14041 cggcgcggcg gtgtgagccg gtgccggagc ggccgacggc gtgggcgcgg gcgccggagc
    14101 cgttgaaggc gcaggcgtgg aagcgggcgc gaccgcatgc gcggccggcg tcgtcgcgcg
    14161 cttggggcgc ggacggt
//
LOCUS       DASVXS010000118         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_173555, whole genome shotgun sequence.
ACCESSION   DASVXS010000118 DASVXS010000000
VERSION     DASVXS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173555"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VF752_06530"
     CDS             complement(<1..1446)
                     /locus_tag="VF752_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855240.1"
                     /translation="MSKLPSWRRYLRFWGPDLDADIDEELRFHLEQLTAAYEANGLSH
                     QEAVRAARVRFGDTAAIGSSMRQYDKESVRRQARAERVRLLGQQLLVALRGLRRAPSF
                     TIAVVLTLALGIGLSTAVFDVASTLLLRPLPVRDEGRLVLLSGRTRDGTIANYPLPLD
                     EAREFARASSVLAGTALVSYYGAVPQPVVRDDRIVPLKVAQVSGNFFALLGMRPTIGR
                     LLRDEDDVVGAGPVAVLSHDAWMRNYGGDSAVLGTLLRVHSTGIAYVIVGVATEGLGY
                     PGQTDVWVPIATSTPHGAERFVMVNLLGRLRAGRSAEQAREELATYYTRPGAPTSEAD
                     LSAAATGLRTVVLGDVRPAVIVFAVAAALLLAIACINVGTLMLVRVLSRAPELAVRSA
                     LGARRWHLAGLLMTEGALLAGVGGSAGVALAVLVLKAFRTLAPATLPRLSEVRSSAQT
                     LGVAIVVTAFALLAFAVVPAYRHSRVDAGEVL"
     gene            complement(1454..1813)
                     /locus_tag="VF752_06535"
     CDS             complement(1454..1813)
                     /locus_tag="VF752_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8855241.1"
                     /translation="MQGDAMAGDSGDLLQGTLAILILKALLGGAMHGYGIARWVEKTT
                     DDVLRIEEGSLYPALRRVEARGWITSDWGLSENNRRARFYSITPVGRKHLRAEVAHWT
                     LYVAAIQRVLRAAPAVG"
BASE COUNT          387 a          744 c          753 g          312 t
ORIGIN      
        1 tagcacctcg ccggcgtcga cgcgcgagtg ccggtacgcc ggaacaacgg cgaaggcgag
       61 cagagcgaac gcggtgacga cgatcgccac gccgagtgtc tgcgcgctcg agcgtacctc
      121 gctcagccgg ggaagcgttg cgggagcaag cgtacgaaac gccttcagca cgagcacggc
      181 gagcgcgacg ccggcgctgc cgcccacgcc ggcgaggagc gctccctcag tcatgagcag
      241 gccagcgaga tgccagcgcc gcgccccgag tgccgaccgc accgcgagct cgggcgcgcg
      301 actgagcacc cgcacgagca tcagcgtccc cacgttgatg caggcaatgg cgagaagcaa
      361 cgcggccgcg actgcgaaca ctattactgc gggccgcacg tcgcccagca ccaccgtccg
      421 gagaccggtc gcagcagcgg agaggtctgc ctcgctcgtc ggagcgcctg gtcgcgtgta
      481 atacgtcgcg agctcctcgc gcgcctgctc cgcgctgcgt ccagcccgca gacggccgag
      541 aagattgacc atcacgaagc gctccgcccc gtgcggcgtc gacgtagcga ttggtaccca
      601 gacgtcggtc tggccgggat acccaaggcc ctcggtggcg accccaacga tgacatatgc
      661 gatcccggta ctgtgtacgc gaaggagcgt cccaaggacc gcggagtcgc caccataatt
      721 gcgcatccag gcgtcgtggc tcagcaccgc gaccggtcca gcgccgacga catcgtcttc
      781 gtcgcgcagt agccgaccaa tcgtcggccg catgccgagg agcgcgaaga agttgcccga
      841 gacctgcgcg accttcagcg gtacgatgcg gtcgtcgcgg acgacaggct gggggaccgc
      901 gccgtaatag ctgaccagcg cagtgccggc gagaacactc gacgcgcgcg caaactcgcg
      961 ggcctcgtcg agtggcagcg ggtagttcgc gatcgtgccg tcgcgcgtac gtccggaaag
     1021 caggaccagc cgcccctcat cgcgcaccgg gaggggacga aggagcagcg tgctcgcgac
     1081 gtcgaacacc gccgttgaga ggccgatccc gagcgcgagc gtcaggacca ccgcgatcgt
     1141 gaagctcgga gcgcgacgga ggccgcgcag ggcgacgagc agctgctgtc cgagcagtcg
     1201 cacccgctcg gcccgcgctt gccgccgcac actttccttg tcgtactggc gcatcgagct
     1261 gccgatcgcc gccgtatctc cgaaacggac ccgcgccgcc cgcaccgcct cttgatgcga
     1321 gagtccgttc gcctcgtagg cagcggtgag ctgctcgagg tggaatcgaa gttcctcgtc
     1381 gatatcggcg tcgaggtccg gcccccagaa gcggaggtac cggcgccagg aaggaagctt
     1441 ggacaaccgc gcctcagccg accgcagggg ccgcgcgcag cacccgctgg atcgccgcga
     1501 cgtagagcgt ccagtgcgcg acctcggcgc gcagatgctt acggccgacc ggcgtgatgc
     1561 tgtaaaagcg ggcccgccgg ttgttttccg acaggcccca gtccgaggtg atccacccgc
     1621 gggcctcgac gcgccggagg gcggggtaga gtgagccctc ctcgatgcga aggacgtcgt
     1681 cggtagtttt ctcgacccag cgtgcgatgc cgtaaccgtg catcgccccg ccaaggagcg
     1741 ctttcaggat caggattgcg agcgttccct ggagcagatc cccagaatca ccggccatgg
     1801 cgtccccttg caaggctagg ggaagtatag ggcagccgtg gcgcgccgca agcccgatct
     1861 atccaacgac gcttacggct tccgcgagct tgccgggtct cgtcgctctc gccgacgggg
     1921 cgctcaggaa gggcgcgcga gacttggcag aagtcgcggg acgagtcgcg ggacgtgggg
     1981 cgttgtcggg tcctttcgcg tgtactgttg gggcggaact cgtaggggaa cgtgatgccg
     2041 agtcaccgcc gttaccgttc tgaaaatccg cgtgtcggcg gttcgattcc gtccctggcc
     2101 acctaggacg aagaggaaaa aacagaggag cgcccgggct cgagcccggg cgctcctctg
     2161 ttttacaaag gtcgcgcccg gaaggcggtc tgctcc
//
LOCUS       DASVXS010000119         3910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1219284, whole genome shotgun sequence.
ACCESSION   DASVXS010000119 DASVXS010000000
VERSION     DASVXS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219284"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VF752_06540"
     CDS             complement(<1..891)
                     /locus_tag="VF752_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8855242.1"
                     /translation="MRQKTPETYEEKLAQLQELRDAAIHSASEQAVEKQHAKGKLTAR
                     ERIDKLLDPGSFKELDTFVRHRTSDFDMLKNRPWADAVVTGHGTIDGRNVCVFSQDFT
                     VFGGSLGEVMAEKMCKVMDLAAKIGCPVIGLNDSGGARIQEGVVSLGAYGDVFVRNVQ
                     CSGVIPQISVIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPDVIKTVTGEEPTFEE
                     LGGAMTHNTKSGVAHFAADDEEQCLDDVRYLMSFLPLNNLEMPPRLDTGDDPERMDEE
                     LDHIVPDNPNKPYDMRDVIHR"
     gene            953..2782
                     /locus_tag="VF752_06545"
     CDS             953..2782
                     /locus_tag="VF752_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX8855243.1"
                     /translation="MSRKPILAAFACAAVVLLAPAGAAARAAVPAERALYQDGPNWRY
                     LLDRGWTTRADKGDRGLAAGWQRPGFRAGFRRVSIPNAFNARNLSAAGERSWVQWYRT
                     SFRLPDAPRSAAWRLRFESVNRRADLWLNGRKIGWHEGGYLPFELTASDLRRDGDNEL
                     VVRVDGRESKTDIPPAGRDRGWWNYGGILREVYLRRAGSMDLGDPQVVAKPGQPVRFS
                     ASLRNTTRRTQPSRALLTASGPNGFSKVVSVPSTGGVRPGRLTRIRASFEIPEAALWS
                     PESPSLYELRVKVAGGQQTAIHFGVREFTRDGQGRAFLNGRPLTLRGASFHEDLPRVG
                     AALTAAGEDQIAVQLSDLGADFARQHYPPHPRLLEAFDRLGIVFWEQIPVWRMRGTAE
                     LRSRKVRATAEKRLTEAILQDRNHASVMAWSIGNEILRGGAPEERYIRDSRKISDRLD
                     GTRFLAIDKSLSPVDQIPDYYRLLDAFGVSDYLGWYNGTSVDELPAALDAVRSKLPGV
                     ALFATEFGAEANRDGAPSERGTFAFQSDFLDRTLSVFDATPYVNGAIAWLLRDYPVRP
                     GWSGGNPDPSPPYSTKGLLGLDGSEKPAWEVVRRHFQALPRTQ"
     gene            2825..>3910
                     /locus_tag="VF752_06550"
     CDS             2825..>3910
                     /locus_tag="VF752_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855244.1"
                     /translation="MIRARFAAALAGAAVVVTTAVAMGAAPPSNAPVQLLLVPSTPHA
                     QDALRGTSARVIARYGAFTLVQAQGEDADDLKRAGAERRDDLQRVTLNGRSIDPTGRT
                     SLAGKRRVDRSGLALLQFVGPVKDAWLDNVRATGVKVVTYMPEDSYLVHGSASSLEKL
                     SDLLGSDPAVRAVMPLARTDKLGKGLRSRGRQMAAVQTVAGSTGTAARRAVRRAGTEL
                     RQGPAISGTVSQVVTADAAKLSALASNPAVVAVQPYKSPKLMDERSTQIMAGAIDGSG
                     QPTGPGYLSFLTSKGLGSALSFGIDVTDEGLDKGVLPPPAGSHPDFYVDGNTANATRI
                     QYETNWTMDDPDARDCGGHGTNVASIAA"
BASE COUNT          595 a         1325 c         1385 g          605 t
ORIGIN      
        1 acggtggatc acatcgcgca tgtcgtacgg cttgttcggg ttgtcgggga cgatgtggtc
       61 gagctcctcg tccatgcgct cggggtcgtc gcccgtgtcg aggcgcgggg gcatctccag
      121 gttgttgagc ggcaggaacg acatgaggta gcggacgtcg tcgaggcact gctcctcgtc
      181 gtcggccgcg aagtgcgcga cgccggactt cgtgttgtgc gtcatcgcgc cgccgagctc
      241 ctcgaaggtc ggctcctcgc ccgtcaccgt cttgatcacg tcgggacccg tgatgaacat
      301 gtgcgacgtc tccttgacca tgaagatgaa gtccgtcatg gccggggagt agacggcgcc
      361 gccggcacag gggcccatga tcacgctgat ctgcgggatc acgccggagc actgcacgtt
      421 gcgcacgaac acgtcgccgt aggcgccgag agagaccacg ccctcctgga tgcgggcgcc
      481 gccggagtcg ttcaggccga tcaccgggca gccgatcttg gccgccaggt ccatgacctt
      541 gcacatcttc tcggccatca cctcgccgag cgagccgccg aagaccgtga agtcctgcga
      601 gaagacgcac acgttgcggc cgtcgatcgt gccgtggccg gtcacgactg cgtcggccca
      661 cggccggttc ttcagcatgt cgaagtcgga ggtgcggtgg cgcacgaagg tgtcgagctc
      721 cttgaacgag cccggatcga gcagcttgtc gatccgctca cgggcggtga gcttgccctt
      781 ggcgtgctgc ttctcgacgg cttgctcgga agcagagtgg atcgcggcat cgcgcagctc
      841 ctgcagctga gcgagcttct cttcgtaggt ttccggcgtc ttctggcgca tggggcgccg
      901 cattctagga cgccaccgca gcgggcgcgg ttagcgtgtg ttgcaggtgc cgatgtccag
      961 gaagcccatc ctggccgcgt tcgcgtgcgc ggctgtcgtg ctgctcgcgc ccgccggcgc
     1021 cgccgcaagg gcagccgttc ccgccgagcg cgcgctctat caggacgggc cgaattggcg
     1081 ctacctgctc gaccgcggct ggaccacgcg cgcggacaag ggcgaccgcg gcctggccgc
     1141 cgggtggcag cggcccggct tccgggcggg cttccggcgc gtgtcgatcc cgaacgcatt
     1201 caacgcgcgc aatctcagcg ccgcgggcga gcgctcctgg gtgcagtggt accggaccag
     1261 cttccggttg ccggacgcgc cccgctccgc ggcgtggcgg cttcgcttcg agtcggtgaa
     1321 ccggcgcgcg gacctgtggc tgaatggccg caagatcggt tggcacgagg gcggctacct
     1381 gccgttcgag ctgacggcat cggatttgcg gcgcgacggc gacaacgagc tcgtcgtgcg
     1441 agtggacggc cgcgagagca agaccgacat cccgcccgcg ggacgcgacc gcggctggtg
     1501 gaactacggc ggaatcctgc gtgaggtcta ccttcgccgc gccggatcaa tggacctcgg
     1561 cgatccgcag gtggtggcca agccagggca gccggtcagg ttcagcgctt cgctgcgcaa
     1621 cacgacgcgc cgcacgcagc cgagccgtgc gctgctcacg gccagcggcc cgaatggctt
     1681 cagcaaggtg gtgtcggtgc cgtccaccgg cggcgtgcgc cccgggcggc tcacccgcat
     1741 ccgcgccagc ttcgagatcc cggaggccgc gctctggtcg ccggagagcc cgtcgctgta
     1801 cgagctgcgc gtgaaggtgg ccggtggtca gcagaccgcg atccacttcg gcgtacgcga
     1861 gttcacgcgc gacggccagg gccgcgcatt cctcaacggg cgccccttga cgctgcgcgg
     1921 cgcgagcttc cacgaggacc ttccgcgcgt tggcgcggcg ctcacggcgg cgggcgagga
     1981 tcagatcgcc gtacagctca gcgacctggg cgcggacttc gcacgtcagc actatccgcc
     2041 gcacccgcgg ctcctcgagg cgttcgatcg gctagggatc gtcttctggg aacagatccc
     2101 ggtctggcgg atgcgcggca cggcggagct gcgcagccgc aaggtccgcg cgaccgccga
     2161 gaagaggctc accgaggcga ttctgcaaga ccgcaaccac gcttcggtga tggcatggag
     2221 catcggcaac gagatcctgc gcggcggggc gcctgaggag cggtacatcc gtgactcccg
     2281 gaagatcagc gaccggctcg acgggacgcg cttcctcgcc atcgacaagt cgctctcgcc
     2341 ggtcgaccaa atacccgact actaccggct gctcgacgcc ttcggggtca gcgattacct
     2401 cggctggtac aacggcacgt cggtcgacga gttgccggcg gccctcgacg cggtgcgctc
     2461 caagctgccc ggcgtcgcgc tcttcgcgac cgagttcggc gccgaggcga atcgcgacgg
     2521 cgcgccgagc gagcgcggca cgtttgcgtt ccagagcgac tttctcgacc gcacgctgtc
     2581 ggtgttcgac gccacgccct acgtgaacgg ggccatcgcg tggctgctgc gcgactaccc
     2641 ggtgcgccct ggctggagcg gcggcaaccc cgatccatcg ccgccataca gcacgaaggg
     2701 cttgctcggg ctggacggga gcgagaaacc ggcgtgggaa gtggttcggc ggcattttca
     2761 agcgctgccc aggactcaat aagcgctaag ggaatctggc cgaacgtccg aatagcttct
     2821 tgaagtgatc cgcgcccgct ttgcggcagc cctggcaggg gccgcggtgg tcgtcacgac
     2881 cgcggtcgcc atgggagccg cgccaccctc caatgccccg gtacagctgc tgctcgtgcc
     2941 gtcgacgccg catgcccagg atgcgctgag aggcacgtcc gcgcgcgtga tcgcgcgcta
     3001 cggcgcgttt acgctcgtgc aggctcaggg tgaggacgcc gacgatttga agcgggccgg
     3061 cgcggagcga cgcgacgacc tccagcgggt caccctcaac ggccgctcga tcgatccgac
     3121 cggccgtaca tcgctcgccg gcaagcggcg cgtggaccgg tccgggctgg cgctcctgca
     3181 gttcgtcggg ccggtaaagg acgcgtggct cgacaacgtg cgcgcaaccg gcgtgaaggt
     3241 ggtcacctat atgccggagg actcgtacct cgtgcacggc tcggcgtcgt cgctcgagaa
     3301 gctgagcgac ctgctcggca gcgacccggc cgtccgggcg gtgatgccgc tcgcgcgcac
     3361 cgacaagctg ggtaagggtc tgcgctcgcg cggccgccag atggcggccg tgcagaccgt
     3421 tgcgggctcg accggaacgg cggcccgccg cgccgtcagg cgagctggca ccgagctgcg
     3481 ccaggggccg gcgatcagcg gcacggtgag ccaggtcgtc accgcggatg ccgcgaagct
     3541 gagcgcgctc gcctcgaacc cagccgtcgt ggcggtgcag ccctacaagt cgcccaagct
     3601 gatggacgag cgctcgacgc agatcatggc cggcgcgatc gatggatcgg ggcagccgac
     3661 gggaccgggt tacctcagct tcctcacgag caaaggtctt gggagcgcct tgagcttcgg
     3721 gatcgacgtg accgacgagg gtctcgacaa gggcgtgttg cctccacccg cgggctccca
     3781 cccggacttc tacgtggacg gcaacacagc caacgcgacg cgcattcagt acgagaccaa
     3841 ctggacgatg gacgaccccg acgcgcgcga ttgcggtggg cacggcacga acgtggcgtc
     3901 gatcgccgcg
//
LOCUS       DASVXS010000120        16550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_386576, whole genome shotgun sequence.
ACCESSION   DASVXS010000120 DASVXS010000000
VERSION     DASVXS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16550
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386576"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF752_06555"
     CDS             complement(<1..250)
                     /locus_tag="VF752_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX8855245.1"
                     /translation="MEGARHPITLVVRDDLERTRLTVFFRLILAIPHIIWFWLWSIAA
                     LFAAIANWFATLVEGRSPAGLHAFLSRYLRYGTHFFSYL"
     gene            complement(279..1151)
                     /locus_tag="VF752_06560"
     CDS             complement(279..1151)
                     /locus_tag="VF752_06560"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEX8855246.1"
                     /translation="MGLTDEVRRSCAEIAANARHVRIDIDRLGDIEPGPPLHLDPERH
                     YLEGSREDVAMYMLTVDAVNFGSGWFPTLRKRPGSSGYYTIAWSLADRFRERGPWSPE
                     ELRSISADEVAEVCGQERGHELMTLYAEALSQLGRFLGERSALEVIDAARGSAERLAE
                     QLAHGMEFFHDVGFYKRAQITANDLTLAGVAAFEDIDRLTIFADNLVPHVLRMDGVLV
                     YEPALAELIDAERLLPPGEKEREIRACSVHACERIAERLGVPPRVLDVWLWDRGQGPR
                     YKARPRHRTRTVYY"
     gene            1212..3395
                     /gene="pcrA"
                     /locus_tag="VF752_06565"
     CDS             1212..3395
                     /gene="pcrA"
                     /locus_tag="VF752_06565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEX8855247.1"
                     /translation="MTTAAPTDRLLEGLNPQQREAVLHETGPLLILAGAGSGKTAVLT
                     RRIAYLVQTKTAQPGEILAITFTNKAAQEMRERVEQLVGSRTRAMWVMTFHSACVRIL
                     RAEAARLGYTRGFTIYDEADSLRLIKQCLEELDVDPKRFTPRAIKRQISDAKNQLLDA
                     AAYREKVSSFFEQTAADVYEHYESRIHSMNAMDFDDLLMRCVDLFQLFPEVLQRYQHT
                     FRQILVDEYQDTNRAQYRWLQLLSAEHRNLSVVGDDDQSIYRFRGADVRNILDFEDDF
                     PDARVVKLEQNYRSTQTILSAANAVIGNNRERKQKSLWSDLGEGDPVRVRELEDEHAE
                     ARFVINEIERVVDHGGSRDEIAVFYRTNAQSRVLEDMLTRYKLPYQVIGGTKFYERAE
                     IKDAIAYLTLLVNPSDAVAFQRIVNSPRRGIGDTTQGRIVGYANTIGEPVWDVAMKAD
                     EVPNLGAAAVKAVDRFMSVMERLAERVEHASLGDLLQETLNETGYLEALEAERTIEAQ
                     GRIENLEELVGVAREYDATSEEPSLEEFLQQIALFSDQDAIRDDEGTVTLMTLHNAKG
                     LEFPVVFIIGCEEGVFPHSRAIEEGDLEEERRLAYVGITRAKRELFMTFARTRTLYGG
                     RDWNLPSRFLDELPAELTDVQHQEERRTAFSRWGSGGGAPSTAPARREPTPGASFSLG
                     DDVVHASFGEGVVVGVEPGGVVVVRFAADGAEKKLMADYAPLKKK"
     gene            3407..4279
                     /gene="folD"
                     /locus_tag="VF752_06570"
     CDS             3407..4279
                     /gene="folD"
                     /locus_tag="VF752_06570"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX8855248.1"
                     /translation="MAAQIIDGKAIAAEVRARVHEDVAELVEEYGVRPGLATVLVGED
                     PASQVYVRNKRRQSEEVGISSIHHDLPEDASQSEVDELLRHLNEDDTVHGVLLQLPVP
                     RQIDGGAMTRLIAPAKDVDGLTPTNAGLLVQGLEGLVSATPSGVMELLRAHEVELRGA
                     EAVVVGRSTLVGKPVAALLLNADATVTSCHSKTRDLAEVCRRADVLIAAVGVPRLVQG
                     DWVKEGAVVIDVGINRLDEGLVGDVDFDAAKERASLISPVPGGVGPMTIAMLLHNTVT
                     AARRQLARARVGAS"
     gene            4290..4739
                     /locus_tag="VF752_06575"
     CDS             4290..4739
                     /locus_tag="VF752_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855249.1"
                     /translation="MKLSRTRASDWLAALAGVALIGLMFVDWFGSASAWQSLTVVDVL
                     LALCGLTGVTLFVATATQRSNAVPIAVSSLTGLAGMLATVLVAIKLIFPPDGASVSTG
                     AWLGLAAAAVLAAAGWESMREERRGNPPYDRSGIRRVSADELREGLR"
     gene            4766..5392
                     /locus_tag="VF752_06580"
     CDS             4766..5392
                     /locus_tag="VF752_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855250.1"
                     /translation="MLAWVAALALLVFSAMDWWSTVRGDELRQEQKSAVQQAPISGEI
                     PNATRDDARIAAQGQEKNAWQAGHLIDKLILALLLATVAAATFAAMARAADRRFESPW
                     TPSALAAGLALLTALLIAYRIVNEPGIDSITIVKLGSVLSLCAATFVAVGSGIAMQRE
                     IDGRAYKDLPEPGGNGRTPSGTGGDGAGPSSDGDGRGPAPRPTAGAAG"
     gene            complement(5389..7296)
                     /locus_tag="VF752_06585"
     CDS             complement(5389..7296)
                     /locus_tag="VF752_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855251.1"
                     /translation="MTRARAQRAGALSAREARIFRAFCEALIPDGGHLPQAEGGDGVP
                     VVEPVGEYLAHVPALMRALFKASLRALELSPFPRRFSRLPLERRAEHLESLESSRLSP
                     FRDLFLVLKTLAALGYTRDAAVQEAVGSFTTCVTASGAAPERDAPPLDPAAMRAPDGV
                     ERCDVVVVGSGAGGASAARVLAERGLDVMVVESGEYHDASTYTTDPLEAIPQLYRDGG
                     MTACEGRPVIPLPLGHCVGGTTVINSGTCFRTPDSVLTRWRSEFGIDWAGDLEAEFEA
                     VERALAVRPVDADTAGRNAALCRVGAERLGASNAPLARNAGDVVCCGSCPVGCRLDAK
                     QAMHVSELPRAVAAGARIRAGSRVERVIVEGGRAVGVACDGYEVRARAVVLAAGAIGT
                     PELLMPQGLAATSGHLGRHLHIHPACWVGGLFDEEVRGWDGIMQSWLVNEWENRGVNL
                     EATCTPLTFGGHWMRGAGQPYKERLERYANVAMIGVMISDRSEGRLTTIGGRARIRYR
                     LDRADQEKLRFGIARAADVHFAAGASEVYPQVGRVAVLAPGEQDRRVEQARFRPGEFR
                     LEGFHPMGTARMAAAPAAGVVAPSGEAHDVPGLYVADASVFPTALGVNPMITICAVAR
                     RIAAGLAERLS"
     gene            7356..8714
                     /locus_tag="VF752_06590"
     CDS             7356..8714
                     /locus_tag="VF752_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8855252.1"
                     /translation="MYRWLAGTAIFVALCAPAVAHAQPPVRHVFVIVLENKGYDETWG
                     PSSKAPYLSKTLTSMGALIPGYYGIGHLSLGNYIAMVSGQAPNPQTQSDCQLFTEFLP
                     GVPAPSDGQFIGSGCVYPAAVKTVANQLDAKGLSWKGYMEDMKTPCRRPASGTHDTTQ
                     EAKVGDQYAARHNPFVYFHSIIDRPACAKNDVDFSQLSSDIASPATTASYSFITPNLC
                     NDAHDDPCVDNRPGGLVSADAWLKQVVPEILSSPGYQDNGMLVITFDEAENGDASACC
                     NEQPGYNTPNPGGPTPGKGGGKTGTVVISQFTKPGTVSKFEYNHYSFLRSVEDLFGLA
                     HLGYAGQPGLRPFGDDIYNATVAPAGGTPGARKLRARQLRLKAAPRRLEAGRINTLRV
                     RTDAAATVTVGGACKRSSRRTRAHATKRWRIRAPKAGRSCTVTASRRGWSAANRTLRS
                     RR"
     gene            8758..9042
                     /gene="gatC"
                     /locus_tag="VF752_06595"
     CDS             8758..9042
                     /gene="gatC"
                     /locus_tag="VF752_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX8855253.1"
                     /translation="MIGREQVLHVARLARLELSEAEVERFSEELSTVLDHIEQINQLD
                     LDGVEPTSHVIQVENVLREDVPRPCLPREKALEQAPDVAAGGFRVPSPGA"
     gene            9039..10451
                     /gene="gatA"
                     /locus_tag="VF752_06600"
     CDS             9039..10451
                     /gene="gatA"
                     /locus_tag="VF752_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX8855254.1"
                     /translation="MTGLIELTAATAAERVRAGEVSPRELFDAYRERAASEDLGAFLW
                     VADEAPDVPADAPLGGVPLAVKDLFCVEGVPSSAGSRIIEGYRPPYTATSVERVVRAG
                     APVLGKTNMDEFAMGSSNENSGYGPVRNPWDPERVPGGSSGGSAAAVAAGIAPWANGT
                     DTGGSIRQPAALCGIVGLKPTYGAVSRYGMIAFASSLDQCGPLTRDVTDAALLFRHMV
                     GARDECDSTHLEYPEEVRLPSRDSLEGLRFGVWGLGEEGTEPGVDEAVRAAIATIESL
                     GGAVDEIELPHAPHGLSAYYVIAPAEASANLARYDGVRYGRRAEDFDDLISMYERTRA
                     EGFGAEVKRRIMLGTYALSSGYYEAYYGRAQRVRTKIADDFREAFGRVDFVVTPTSPT
                     VAFKLGERTANPLAMYLSDYFTVPMSLAGLPAISIPCGLSQGLPVGLQLAGPAFSENR
                     LLDAAYAIERAIGFERRSFS"
     gene            10448..11896
                     /gene="gatB"
                     /locus_tag="VF752_06605"
     CDS             10448..11896
                     /gene="gatB"
                     /locus_tag="VF752_06605"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8855255.1"
                     /translation="MTVSGEPWADRHWEPVIGLEIHVQLKTRTKMFCGCTLSFGDEPN
                     THTCPICLGHPGTLPTVNAEAVHFGLMIGLALGCEIAPRSIFHRKNYFYPDLPKGYQI
                     SQYDIPLASNGRLGDVRIHRAHLEEDAAKLVHAGSSGRIHGSEASIVDFNRGGTPLVE
                     IVSEPDLRSASEAADWARLLRATLKRLGVSDVNMEEGSLRVDANVSLRRAGHATFGTK
                     TELKNMNSFRFLERGIAAELERQYEILHNDGTVVQETLHYDPVTGSISSLRSKEEAHD
                     YRYFPEPDLVPIAPTETMMERARAALPELPAARAERFERDLSLPADTARLLAFRSELG
                     DFFEQALAADSGDPRVVANWVTVELVPRLSGEDPFESRVTPGALSELVRMVGAREVSQ
                     SAAKEVLDVLAAEGGSPSEIVSSRGLGMAGDDELGSLVDRAIEANPRAAEEYKAGKQK
                     AVGAIVGFVMRETKGRADGGEVNRLIREKLGA"
     gene            12033..12485
                     /locus_tag="VF752_06610"
     CDS             12033..12485
                     /locus_tag="VF752_06610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8855256.1"
                     /translation="MAKLIDLARGRRRVVELGTGTGWTAIALALAATQEPREVITYDP
                     IPRPERDIYLRLVPGQVQARIRFIEQGAEEGPRPGAPPADLVFLDSSHERDETLTTFR
                     AWEQALAPGAVVAFHDYDEPAYPGVTEAVEELGLNGQVYGHLFIWRAP"
     gene            12582..13523
                     /locus_tag="VF752_06615"
     CDS             12582..13523
                     /locus_tag="VF752_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8855257.1"
                     /translation="MRPKSHPRPKDVADRASQQHGVVTYRQLRRMGFSHEAIKRRIRV
                     GEFHPLYRGVYAVGHTAMTRRGLLMAAVYACGEGAVASHWDAAELWGLSRNGGKTFHV
                     TVVAKGRKSRKGITLHQVRRLDRADWTEVDNVRVTSLARTFLDQAEVVNRRQLERQLE
                     TAERQQIFDLRAIKDTCRRNPGRHGIPILIAAVNELMPEALHSREELERLFLDFCREH
                     NIPMPAVNVVVEGYSVDALWRAQRVVVELDSREFHLNRKAFEEDRRRDGALQIAGYRV
                     LRITWRRLTQEPAGVASDILTLLAHAPAASSVSSQLP"
     gene            complement(13481..13873)
                     /locus_tag="VF752_06620"
     CDS             complement(13481..13873)
                     /locus_tag="VF752_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855258.1"
                     /translation="MPSGQVELARRLYTAIESADLDAMLEFVAPDFELHTSGEFPGDA
                     AVYRGRDGYVRFWQEWWDLWSEFRFAVEELRECGDVVAALYWLEGETRNGPRVRRRGG
                     EVVRFRGEVMVRVDAYGSWEETLEAAGA"
     gene            complement(13883..16168)
                     /locus_tag="VF752_06625"
     CDS             complement(13883..16168)
                     /locus_tag="VF752_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855259.1"
                     /translation="MASTETAPAAREAPDLDTLRSIERRVLWLATAMVHQANRVRPNR
                     SGVKVGGHQTSSASMVSIMTALWFSLLEAPDRVSVKPHASPVLHAINYLLGRLDRRYL
                     TTLREFEGLQSYPSRVKDPDPVDYSTGSVGIGATAPVWGALAHRYVAGHFEVPIGGRQ
                     IALIGDAELDEGAVWEAIADPMVARLGEVMWVVDLNRQSLDRVVPDIAAGRLRSMFEA
                     AGWHAVTVKYGSNLRALFERSGGRALERRIDEMSNEEYQRLLRLPASDLRERLPGRGR
                     GRRELTKLVGQLDDSELIAAFRDLGGHDLGALLEGYRDADAVRDRPSIVFAYTIKGWR
                     LATEGHPSNHSALLTPGQMEQLAGELGVDLDDPWAGFPIDSDEGELLADTARRLERPA
                     VEVGEPPPVPDSLGREHVGTESTQKALGRLFFDLTQSAPEVAARMVTVSPDVASSTNL
                     GGWINHVGIWNLGDRIDWFADDPETLVRWRESDHGNHIELGIAETNLVGLLGELGATW
                     SRDGQPLLPVGTIYDPFVGRALEPWSFGIYAGGQSILVGTPSGITLSPEGGAHQSIVT
                     PSIGIEQPGCVAWEPAFGQDFEWAFLHALSRLGRRGGSSAYFRLSTRPIDQTLRGPVE
                     RSHALAGGYRLRSGDRDVTIVCMGAVVPEAVSAAEELGGAEVICVTSADLLFRALQAR
                     QGLGDGDSWILDELFPAARPLVTIVDGHPHTLAFLAGVHGVPIASLGVRDFGQSGDVD
                     DLYEHYGIDAETIVGAALDLL"
     gene            16250..16474
                     /locus_tag="VF752_06630"
     CDS             16250..16474
                     /locus_tag="VF752_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855260.1"
                     /translation="MSRLIRMDATGHSKLAEWTAGDEASVEAAVEAFRRELDEGYVAV
                     VPEGEGEATQVRELPLDAHLVILRRPIAGG"
BASE COUNT         2614 a         5770 c         5804 g         2362 t
ORIGIN      
        1 ataggtacga gaagaagtgc gtgccgtagc gcaggtagcg gctgaggaac gcgtgcagcc
       61 cggccggcga cctgccttcc acgagcgtgg cgaaccagtt cgcaatggcc gcgaacaggg
      121 cggcgatcga ccacagccag aaccagatga tgtgcgggat cgcgaggatc aggcggaaga
      181 agacggtgag ccgcgttcgc tccaggtcgt cgcggacaac gagagtgatg gggtgacgcg
      241 ctccctccac tgcaggactt gataccacgc cagggacgtc agtagtagac cgtgcgagtg
      301 cggtgacgcg gccgggcctt gtagcgcggc ccttggccgc ggtcccagag ccacacgtcg
      361 aggacccgcg gaggcacgcc cagccgctcg gcgatccgct cgcaggcgtg gacggagcac
      421 gcgcggatct cccgctcctt ctcacctggc ggcagcagcc gctcggcgtc gatcagctcc
      481 gcgagcgccg gctcgtacac gagcacgccg tccatccgca gcacgtgcgg cacgaggttg
      541 tcggcgaaga tcgtgaggcg gtcgatgtcc tcgaacgcgg cgacgcccgc gagtgtgagg
      601 tcgttcgccg tgatctgggc ccgcttgtag aagcccacgt cgtggaagaa ctccatgccg
      661 tgggcgagct gctcggcgag gcgctcggcc gagccgcggg cggcgtcgat cacctcgagc
      721 gcgctgcgct cgccgaggaa gcgcccgagc tggctgagcg cttccgcgta gagagtcatg
      781 agctcgtggc cacgctcctg gccgcacacc tcggccacct cgtcggcgga tatcgatcgc
      841 aactcctcgg gcgaccaggg gccacgctcc ctgaaacggt ccgcgagcga ccaggcgatc
      901 gtgtagtagc cggagctgcc gggccgcttg cgcagcgtcg gaaaccagcc cgagccgaag
      961 ttgaccgcgt cgaccgtgag catgtacatc gccacgtcct cgcgagagcc ctcgaggtag
     1021 tgacgctcgg gatcgaggtg cagcggcggc ccgggctcga tatctccgag ccggtcgatg
     1081 tcgatccgca cgtgccgcgc gttcgcggcg atctcggcgc atgagcgcct cacctcgtcg
     1141 gttagcccca cggcgcggga ggctacttct gggcgcctgg tcaagccggg cacaggtcta
     1201 tcgtctaatc ggtgaccacc gccgccccga ccgatcgcct gctggagggc ctcaacccgc
     1261 agcagcggga agccgtcctg cacgagacgg gcccgctgct catcctcgcc ggcgcaggat
     1321 ctggcaagac cgccgtgctc acccgtcgga tcgcctacct ggtgcagacc aagacggctc
     1381 agccgggcga gatcctcgcg atcaccttca ccaacaaggc cgcgcaggag atgcgcgagc
     1441 gcgtggagca gctcgtcggc tcgcgcacgc gcgcgatgtg ggtgatgacc ttccactccg
     1501 cctgtgttcg catcctgcgc gccgaggctg cgcgcctcgg ttacacgcgc ggcttcacga
     1561 tctacgacga ggcggactcg ctgcgcctga tcaagcagtg cctcgaggag ctcgacgtcg
     1621 acccgaagcg cttcacgccg cgggcgatca aacgccagat ctcggacgct aagaaccagc
     1681 tgctcgacgc cgccgcctat cgagagaagg tcagctcctt cttcgagcag accgccgcgg
     1741 acgtgtacga gcactacgag agccggatcc actccatgaa cgcgatggac ttcgacgacc
     1801 tgctgatgcg gtgcgtcgat ctgttccagc tcttcccgga ggtgctgcag cgctaccagc
     1861 acacgttccg gcagatcctt gtggacgagt accaggacac gaaccgcgcc cagtaccgct
     1921 ggctccagct gctctcggcc gagcaccgca acctgtccgt cgtcggcgac gacgaccagt
     1981 cgatctaccg cttccgcggc gcggatgtgc gcaacatcct cgacttcgag gacgacttcc
     2041 ccgacgcccg cgtcgtgaag ctcgagcaga actaccgctc gacgcagacc atcctgtccg
     2101 ctgccaacgc ggtgatcggg aacaaccgcg agcgcaagca gaagtcgctc tggtcggatc
     2161 tcggcgaggg cgatcccgtg cgtgtgcgcg agctggagga cgagcacgcc gaggccaggt
     2221 tcgtgatcaa cgagatcgag cgagtcgtgg accacggcgg gtcgcgcgac gagatcgccg
     2281 tcttctaccg cacgaacgcg cagtcgcgag tgctcgagga catgctcacc cgctacaagc
     2341 tgccgtacca ggtcatcggt ggcacgaagt tctacgagcg cgcggagatc aaggacgcga
     2401 tcgcctatct cacgctgctc gtgaacccga gtgacgcggt cgcgttccag cgcattgtca
     2461 actcgccgcg gcgcggaatc ggcgacacca cgcaggggcg catcgtgggc tacgcgaaca
     2521 cgatcggaga gccggtctgg gacgtggcga tgaaggccga tgaggtgccg aacctcggag
     2581 ctgccgcagt gaaggcggtg gaccgtttca tgtcggtgat ggagcgcctt gccgagcgcg
     2641 tcgagcacgc atcactgggc gacctgctcc aggagacgct gaacgagacc ggttacctcg
     2701 aagcgctcga ggccgagcgc acgatcgagg cccaggggcg gatcgagaac ctcgaggagc
     2761 tcgtgggcgt ggcgcgcgag tacgacgcca cctccgagga gccgtcgctc gaggagttcc
     2821 tgcagcagat cgcgctgttc tccgaccagg acgcgattcg cgacgacgag ggcacggtca
     2881 cgctgatgac gctccacaac gcgaaggggc tggagttccc ggtggtgttc atcatcggct
     2941 gcgaggaggg cgtgttcccg cacagccgcg cgatcgagga gggcgacctc gaggaggagc
     3001 gccgcctcgc gtacgtgggc atcacgcgcg cgaagcgcga gctgttcatg acgttcgcgc
     3061 ggacgcgcac gctgtacggc gggcgcgact ggaacctgcc gagccgcttc ctcgacgagc
     3121 tgcccgcaga gctcaccgac gtgcagcacc aggaggagcg gcgcaccgcg ttctcgcgct
     3181 gggggtcggg cggcggtgct ccgtccaccg cgccggcgcg cagagaacca acccctggcg
     3241 cctcgttctc gctcggcgac gacgtggtgc acgccagctt cggcgagggc gtcgtggtcg
     3301 gcgtggagcc gggcggcgtg gtggtggtgc gcttcgcggc ggacggtgcc gagaagaagc
     3361 tgatggcgga ctacgcgccg ttgaaaaaga agtagagagg agttctttgg ccgcacagat
     3421 catcgacggg aaggcgatcg ccgcggaggt ccgtgctcgc gtacacgagg acgttgccga
     3481 gctggtcgag gagtacggcg tgcggcccgg cctcgcgacc gtgctcgtcg gagaggaccc
     3541 cgcctcgcag gtgtatgtgc gcaacaagcg ccggcagagc gaggaggtcg ggatctcgtc
     3601 gatccatcac gacctgcccg aggacgcctc gcagtcggag gtcgacgagc tgcttcggca
     3661 cctcaacgag gacgatacgg tgcacggcgt cctgctgcag ctgccggtgc cgcggcagat
     3721 cgacggcggc gcgatgacgc ggctgatcgc gccggccaag gacgtcgatg gcctcacgcc
     3781 cacgaacgcc ggactcctcg tccagggcct cgaggggctg gtgtcggcca ctccctccgg
     3841 cgtgatggag ctgctgcgcg cgcacgaggt ggagctccgg ggggccgagg cggtcgtcgt
     3901 cgggcgctcg acgctcgttg gcaagcccgt ggctgcactg ctgctgaacg cggacgcaac
     3961 ggtcacgagt tgccattcga agacgcgcga cctcgccgag gtgtgccggc gcgccgacgt
     4021 gctgatcgcc gcggtcggtg tgccgcgcct cgtgcagggc gactgggtga aggaaggcgc
     4081 cgtcgtgatc gacgtgggca tcaaccgcct cgacgagggg ctcgtgggcg atgtggactt
     4141 cgacgccgcc aaggagcggg cgtcgctgat ttctccggtg ccgggcggcg tcggcccgat
     4201 gacgatcgcg atgctgctgc acaacaccgt caccgccgcg cgccggcaac ttgcgcgagc
     4261 gcgcgtgggc gcgagctaac ctgccgcgca tgaagctgtc gcgcacccgc gcgagcgact
     4321 ggctggcggc gcttgcgggc gtcgccctca tcggcctgat gttcgtcgac tggttcggct
     4381 cggcgagcgc ctggcagtcg ctcacggtcg tggacgtcct gctcgccctc tgcggcctga
     4441 cgggtgtcac cttgttcgtg gccacggcga cccagcgctc gaacgcggtg ccgatcgcgg
     4501 tgtcgtctct gacagggctc gccgggatgc tcgccacggt gctcgtcgcg atcaagctca
     4561 tcttcccgcc ggacggggcg agcgtctcga ccggcgcctg gctcggactc gcggcggccg
     4621 cggtgttggc agcggccgga tgggagagca tgcgcgagga gcggcgaggc aacccgccct
     4681 acgaccgctc gggcatccgc agggtgtcag ccgacgagct gagggagggt ttgcgctgac
     4741 cttcgagcgg ctcaaccgcg gcgacgtgct cgcgtgggtg gcggcgctcg cgctcctcgt
     4801 cttcagcgcc atggactggt ggagcacggt gcgcggcgac gagcttcgcc aggagcagaa
     4861 gagcgccgta cagcaggccc cgatctcagg tgagattccg aacgccacac gcgacgacgc
     4921 gcggatcgcc gcgcagggcc aggagaagaa cgcgtggcag gccgggcacc tgatcgacaa
     4981 gctgatcctg gcgctgctgc tcgccaccgt cgccgccgcc acgttcgccg cgatggcgcg
     5041 cgccgccgat cggcgtttcg aatcgccttg gacgccgagc gcgctcgccg caggacttgc
     5101 gctgctcacg gcgctgctca tcgcctaccg gatcgtgaac gagccgggga tcgactcgat
     5161 cacgatcgtg aaactcggct cggtgctctc gctctgcgcc gcgacgttcg tggccgtcgg
     5221 ctcgggcatc gcgatgcagc gagagatcga cgggcgcgca tacaaggacc tgcccgagcc
     5281 gggcggcaac gggcgcacgc cgtccggcac gggcggcgac ggtgccggac cctcgtccga
     5341 tggcgacggc cgcggacccg cgccgcgtcc gaccgcaggc gcggccggtt aactcagccg
     5401 ctcagcgagg cccgccgcga ttcgccgcgc cacggcgcag atcgtgatca tcgggttcac
     5461 cccgagtgcg gtggggaaga ccgacgcgtc ggccacgtag aggccgggca cgtcgtgcgc
     5521 ctcgccgctc ggtgcgacca cgccggcggc cggcgcggcg gccatgcgcg ccgtgcccat
     5581 cgggtggaac ccctcgagcc ggaactctcc cggcctgaag cgggcctgct cgacgcggcg
     5641 gtcctgctcg cctggtgcca gtaccgcgac gcggccgacc tgcgggtaga cctcgctcgc
     5701 tccggccgcg aagtgtacgt cggccgcgcg cgcgattccg aagcggagct tctcctggtc
     5761 ggcgcggtcg agcctgtagc ggatgcgcgc ccgacccccg atcgtcgtga gcctgccctc
     5821 cgagcggtcc gagatcatca cgccgatcat cgccacgttc gcgtaccgct cgagccgctc
     5881 cttgtagggc tgaccggcgc cgcgcatcca atgcccgccg aacgtgagcg gcgtgcaggt
     5941 ggcctcgagg ttcacgccgc ggttctccca ctcgttcacg agccagctct gcatgatccc
     6001 gtcccagccg cgcacctcct cgtcgaagag gccgccgacc cagcaggcgg ggtgaatatg
     6061 gaggtggcgc ccgaggtgcc ctgaggtcgc cgccaggccc tgcggcatca acagctcggg
     6121 cgtgccgatc gcgcctgccg ccagcacgac ggcgcgggcg cgcacttcgt agccgtcgca
     6181 ggcgacacca acggcacggc cgccctccac gatcacgcgt tcgacccgag agcccgcgcg
     6241 gatacgcgcg cctgccgcca cggcccgcgg aagctcggac acgtgcatcg cctgcttggc
     6301 gtcgagccgg cagccgacgg gacagctgcc gcaacacacc acgtcaccgg cgttgcgcgc
     6361 cagcggcgcg ttcgacgcgc cgagccgctc agcgccgacg cggcatagtg ccgcgttgcg
     6421 gcccgcggta tcggcgtcca ccggtcgcac cgccagagcc cgctcgacag cctcgaactc
     6481 cgcttcgagg tcccccgccc agtcgattcc gaactccgag cgccagcggg tcagcacgga
     6541 atcgggcgtg cggaagcagg tgcccgagtt gatcacggtc gtcccgccga cgcagtggcc
     6601 gagcggcagc gggatcaccg gccgtccctc gcaggcggtc atgccgccgt cgcggtagag
     6661 ctgcgggatc gcctcgagcg ggtcggtcgt gtaggtcgac gcgtcgtggt attcgcccga
     6721 ctcgaccacc atcacgtcga gcccacgctc ggcgagcacc cgcgccgccg aggcgccgcc
     6781 cgcgcctgag ccgaccacca ccacgtcgca gcgctcgacg ccgtcgggtg cgcgcatcgc
     6841 cgcggggtcg agtgggggag cgtcgcgctc gggcgcggcg ccgcttgccg tcacgcaagt
     6901 cgtgaaggat ccgaccgcct cctgcaccgc cgcgtcgcgg gtgtagccga gagcggcgag
     6961 cgtcttcagc acgagaaaca ggtcgcggaa agggctgagg cggctcgact cgaggctctc
     7021 gaggtgctcg gcgcggcgct ccagcggcag ccgcgagaag cggcgcggga acggcgacag
     7081 ctcgagcgcg cgcagcgacg ccttgaacag cgcgcgcatc agcgccggca cgtgggcgag
     7141 atactcgccg accggctcga cgaccggcac gccgtcgccg ccctccgcct gcggcaggtg
     7201 gccgccgtcc gggatcagcg cctcgcagaa cgcccggaag atcctcgcct cgcgggcgct
     7261 cagcgcgccg gcccgctgcg cgcgtgcgcg cgtcatcgcc gcggcatcct agaaggtggc
     7321 gccccgtccg cggctcgccg gtactatgcc ccgccgtgta tcgctggctg gcagggacgg
     7381 cgatcttcgt ggcgctgtgc gcgcccgcgg ttgcgcatgc tcagccgccg gtgcgtcacg
     7441 tcttcgtgat cgtgctggag aacaagggct acgacgagac ctggggaccg tcgtccaagg
     7501 cgccgtacct ctcgaagacg ctcacctcga tgggcgcgct cattccgggc tactacggca
     7561 tcgggcatct ctcgctcggg aactacatcg cgatggtgag cgggcaggcg ccgaacccgc
     7621 agacgcagtc ggactgccag ctcttcaccg agttcctgcc gggagtgccg gcgccgagcg
     7681 acggccagtt catcggctcc ggttgtgtgt atcccgcggc ggttaagacg gtcgcgaacc
     7741 agctcgacgc gaaaggcctg tcgtggaagg gctacatgga ggacatgaag acgccctgtc
     7801 gccgtccggc gagcggcacg cacgacacca cgcaggaggc gaaggtcggc gaccagtacg
     7861 cggcgcgcca caacccgttc gtctacttcc actcgatcat cgacagaccc gcctgcgcga
     7921 agaacgacgt ggacttctcg cagctgtcgt ccgacatcgc ctcgcccgcg acgaccgcca
     7981 gctactcgtt catcactccg aacctctgca acgacgccca cgatgatccg tgcgtcgaca
     8041 accggcccgg gggcctcgtc tccgcggacg cctggctcaa gcaagtggtg ccggagatcc
     8101 tgtcgtcgcc ggggtaccag gacaacggca tgctcgtgat caccttcgat gaggccgaga
     8161 acggcgacgc gagcgcctgc tgtaacgagc agccgggcta caacacgccg aaccccggcg
     8221 ggccgacgcc cggaaagggc ggcggcaaga ccggcacggt cgtgatctcg cagttcacca
     8281 agccgggaac ggtgagcaag ttcgagtaca accactactc gttcctccgc agcgtggagg
     8341 acctgttcgg gctggcgcac ctcggctacg cggggcagcc ggggctgagg ccgttcggcg
     8401 acgacatcta caacgccacg gtcgcgcctg cgggtgggac acccggcgcg cggaagcttc
     8461 gggcgcggca gttgcggctg aaggccgcgc cgcgcaggct cgaggcgggc cgcatcaaca
     8521 cgctgcgcgt gcgcacggac gccgcggcga ccgtcacggt cggtggggcg tgcaagcgct
     8581 cgagccgccg cacgcgcgcg cacgcgacca agcgctggcg catccgcgcg ccgaaggcgg
     8641 gacgcagctg caccgtcacg gcgagccgcc gcggctggtc cgctgcgaac cgcaccctgc
     8701 gcagccgccg ctgaccgcgc tccgcccgag cgcggcgcta cctagaatcg ctcgtccatg
     8761 atcggacgcg aacaggtgct gcacgtcgcg cggctcgcgc gactagagct gagcgaggcg
     8821 gaggtggagc gcttctcgga ggagctctcg accgtgctcg accacatcga gcagatcaac
     8881 cagctcgacc tcgacggcgt ggagccaacc tcgcacgtga tccaggtaga gaacgtgctg
     8941 cgcgaggacg tgccacgtcc ctgcctgccg cgcgagaagg cgctcgagca ggcgcccgac
     9001 gtggccgctg gcgggttccg agttccgagc ccgggcgcat gaccgggctg atcgagctga
     9061 ccgcggcgac agccgctgag cgtgtgcgcg cgggggaggt ctcgccgcgc gagctgttcg
     9121 acgcctaccg tgagcgagcc gcgtccgagg atctcggcgc gttcctgtgg gtggccgacg
     9181 aggcgcccga cgtgccggcg gacgccccgc tcggcggcgt gccgctggcg gtgaaggacc
     9241 tcttctgcgt ggagggcgtg ccgagctccg cgggctcgcg catcatcgag ggctaccggc
     9301 cgccgtacac cgccacgtcg gtcgagcggg tggttcgcgc gggtgcgccg gtgctcggca
     9361 agacgaacat ggacgagttc gcgatgggct cgtcgaacga gaactcgggc tacgggccgg
     9421 tgcgcaaccc ctgggatccg gagcgcgtgc ccggcgggtc gagcggaggc tcggccgcgg
     9481 cggtggcggc cggcatcgcg ccctgggcga acgggaccga caccggcggc tcgatccggc
     9541 agcccgccgc gctgtgcggc atcgtcgggc tgaagcccac ctacggagcg gtgtcgcgct
     9601 acggcatgat cgcgttcgcg tcgtcgctcg accagtgcgg gccgctcacg cgcgacgtga
     9661 ccgatgccgc gctgctcttc cgccacatgg tcggcgcgcg cgacgagtgc gattccaccc
     9721 acctcgagta ccccgaggag gtgcgcctgc cgagccgcga ctcgctcgaa gggctgcggt
     9781 tcggcgtctg ggggctgggg gaggagggca cagagccggg cgtcgacgag gcggtgcgcg
     9841 cggcgatcgc gacgatcgag tcactcgggg gcgcggtcga cgagatcgag ctgccgcatg
     9901 cgccccacgg gttgtccgcg tactacgtca tcgcgcccgc ggaggcgagc gcgaacctcg
     9961 ctcgctacga cggcgtgcgc tacgggcgcc gcgccgagga cttcgacgac ctgatctcga
    10021 tgtatgagcg cacgcgcgcc gagggcttcg gcgcggaggt gaagcgccgg atcatgctcg
    10081 gcacctacgc gctgtcgtcg ggctactacg aggcctacta cgggcgcgcg cagcgggtgc
    10141 ggacgaagat cgccgacgac ttccgcgagg cgttcgggcg cgtcgacttc gtggtgacgc
    10201 cgacctcgcc gaccgtggcg ttcaagctcg gcgagcgcac ggccaatccg ctggcgatgt
    10261 acctgtcgga ctacttcacg gtgccgatgt cgctcgccgg cctgccggcg atctcgatcc
    10321 cgtgcgggct gagccagggc ctgccggtcg gcctgcagct ggcgggaccg gcgttcagcg
    10381 agaaccggct gctcgacgcc gcttacgcga tcgagcgggc gatcggcttc gagcggaggt
    10441 cgttctcgtg acggtcagcg gcgagccgtg ggcggatcgt cactgggagc ccgtgatcgg
    10501 gctcgagatc cacgtgcagc tgaagacgcg cacgaagatg ttctgcggct gcacgctgtc
    10561 gttcggcgat gagccgaaca cgcacacgtg cccgatctgc ctcgggcacc cgggcacgct
    10621 accgacggtg aacgccgagg cggtgcactt cgggctgatg atcgggctcg cgctcggctg
    10681 cgagatcgcg ccgcgctcga tctttcaccg caagaactac ttctatcccg acctgcccaa
    10741 gggctaccag atctcccagt acgacatccc gctcgcgtcg aacgggcgcc tcggcgacgt
    10801 gcgcatccat cgcgcgcacc tcgaggagga cgcggcgaag ctcgtacacg cggggtcgtc
    10861 gggacggatc cacggctcgg aggcgagcat cgtcgacttc aaccgcggcg gcacgccgct
    10921 cgtggagatc gtgtccgagc cggacctgcg ctccgcgtcg gaggcggccg actgggcgcg
    10981 gctgctgcgg gccacgctga aacgcctcgg cgtatccgac gtgaacatgg aggagggctc
    11041 gctgcgggtg gacgcgaacg tgtcgctgcg ccgcgcgggc cacgccacgt tcggcacgaa
    11101 gaccgagctg aagaacatga actcgttccg cttcctcgag cgcgggatcg cggccgagct
    11161 ggagcgccag tacgagatcc tgcacaacga cgggacggtg gtgcaggaga cgctgcacta
    11221 cgacccggtc actggctcga tctcttcttt gcggtcgaag gaagaggccc acgattaccg
    11281 ctacttcccc gagccggatc tggtgcccat cgcgcctact gagacgatga tggagagggc
    11341 ccgggcggct ctccccgagc tgccggcggc tcgtgcggag cggttcgagc gcgatttgtc
    11401 tttgccggcc gatacggcgc ggctgctggc gttccgctcc gagctcggcg acttcttcga
    11461 gcaggcgctc gccgccgatt cgggcgatcc gcgggttgtc gccaactggg tcaccgtgga
    11521 gctcgtgccg cgtttgtctg gcgaggaccc gttcgaatcg cgcgtgaccc ccggggcgct
    11581 ttctgagctg gtgaggatgg ttggtgctcg cgaggtctcg cagtctgccg ccaaggaggt
    11641 gctggacgtg ctggctgcgg agggcggctc gccgtccgag atcgtttcct cccgtgggct
    11701 cggtatggcc ggcgacgacg agctcggctc gctcgtggac cgcgcgatcg aggcaaatcc
    11761 cagggctgcc gaggagtaca aggccggtaa gcagaaggcg gtgggcgcga tcgtcgggtt
    11821 cgtcatgcgc gagaccaagg ggcgcgcgga cggcggcgag gtgaaccgcc tgatccggga
    11881 gaagctcggc gcctgagcgc agccgcatcc ggacggcgtg gacgctccgg cgcctgccgc
    11941 caggcgtaat ccgcttctat ctgcgcgcgc tgcgcgcggc ggagcgagcg gacgaccgct
    12001 tcagcctcga gtcggccact cgtgcggacg agctggcgaa gctgatcgac ctggcgcgcg
    12061 gacgcaggcg ggtggtcgag cttgggaccg gcacggggtg gacggcgata gcgctggctt
    12121 tggcggccac gcaggagcca cgcgaggtga tcacctacga cccgatcccg cgcccggagc
    12181 gcgacatcta cctgcggctc gtgcccgggc aggtgcaggc gcggatccgc ttcatcgagc
    12241 agggagcgga ggaaggtccg cggccgggag cgccgccggc ggacctggtc ttcctcgaca
    12301 gctcacacga gcgcgacgaa acgctcacga cgttccgcgc ctgggagcag gcgcttgcgc
    12361 ccggcgcggt ggtcgcgttc cacgactacg acgagcccgc ctacccgggc gtgacggagg
    12421 ccgtggagga gctcggcctc aatggccagg tctacggcca cctcttcatc tggcgcgcgc
    12481 cgtagcggtc gcggtcgagc gggcgcgctg ctgtaacgcc accgttgcgt aaagagcact
    12541 aaaggggggc gagggagggg gtgggtgacc ttagggtcga tatgcgaccc aaaagtcacc
    12601 cacgtcccaa agacgtcgcc gatcgcgcgt cccagcagca cggggttgtc acttatcgcc
    12661 agttgcgaag gatgggattc agccacgagg cgatcaagcg ccgcatccgg gtcggtgagt
    12721 tccatccgct ctataggggc gtctacgccg tgggacacac cgcgatgacg agacgcggtc
    12781 tcctcatggc tgctgtttac gcctgcggcg agggcgccgt ggcgagtcac tgggacgctg
    12841 cggagctgtg gggcctgagc cggaacggcg gcaagacgtt ccacgtgacc gtggtcgcca
    12901 aggggcggaa gagtcggaag ggcatcactc ttcatcaagt ccgtcgtctc gatcgcgccg
    12961 actggacgga agtcgacaac gtccgcgtca ccagcctcgc ccgcaccttc cttgaccaag
    13021 cggaggtcgt gaaccgccgc cagctcgagc gccagctgga aaccgctgag cgccagcaga
    13081 tcttcgacct cagagcaata aaggacacct gccggagaaa cccgggccgc cacggaatcc
    13141 ccatcctcat tgctgctgta aacgagctga tgcccgaggc gctccacagc cgagaggagc
    13201 tcgaacggct cttcctcgac ttctgccgcg aacacaacat ccctatgccg gcggtcaacg
    13261 tggtcgtcga gggctactcg gtcgacgctc tatggcgcgc gcagcgcgtg gtggtggagc
    13321 tcgacagccg cgagttccat ctcaatcgca aggcgttcga ggaggaccgc cgccgcgatg
    13381 gggcgctcca gatcgcgggt taccgagtgc tgcgaatcac gtggcggcgg ctcacacagg
    13441 agcccgccgg cgtcgccagt gacatcctga ccctgctcgc tcacgcgccg gccgcttcga
    13501 gcgtctcctc ccagctcccg taggcatcga cgcgaaccat cacttcaccg cggaaccgca
    13561 cgacctcacc gccgcggcgc cgtacccgcg ggccgttgcg cgtctcgccc tcgagccagt
    13621 agagcgccgc caccacgtcg ccgcactcgc gcagctcttc cacggcgaag cgaaactctg
    13681 accacaggtc ccaccactcc tgccagaacc ggacgtaccc gtcccggccg cggtacacgg
    13741 ccgcgtcgcc ggggaactcg cccgatgtgt gaagctcgaa gtccggcgcg acgaactcga
    13801 gcattgcatc gaggtccgcg ctctcgatcg cggtgtacaa cctgcgcgcc agctcgactt
    13861 ggccgctcgg cacggcggga acctagagca gatcgagggc cgcgcccacg atcgtctcgg
    13921 cgtcgatgcc gtagtgctcg tacaggtcat cgacatcgcc cgactggcca aagtcgcgca
    13981 cgccgaggga cgcgatcggt acgccgtgca cgccggcgag gaacgcgagc gtgtgcggat
    14041 ggccgtccac aatcgttacg agcggccgcg ccgccgggaa cagctcgtcg aggatccacg
    14101 agtcaccgtc accgagaccc tgccgcgcct gcaaggcccg gaacaacaga tccgccgacg
    14161 taacgcagat cacctcggcg ccaccaagct cctcggccgc gctcactgcc tcaggaacaa
    14221 cagcacccat gcaaacgatc gtcacgtcgc ggtccccgga ccggagtcgg tatccgcccg
    14281 caagagcatg cgaacgctcc accggaccgc gaagcgtctg gtcgatcggc cgcgtcgaca
    14341 gccggaagta cgcggagctc ccacctcgcc ggccgagccg cgacagcgcg tgcagaaacg
    14401 cccactcgaa gtcctgcccg aatgccggct cccacgccac acacccaggc tgctcgatcc
    14461 cgatcgaagg cgtgacgatc gactggtgcg cgccgccctc gggcgaaagt gtgatcccgg
    14521 atggggtgcc caccaagatc gactgcccgc ccgcgtagat gccgaacgac caaggctcca
    14581 gagcgcgccc gacgaacggg tcgtagatcg tcccgaccgg caacagcggc tggccgtcgc
    14641 ggctccacgt cgctccgagc tcgccgagca ggcccacgag gttcgtctcc gcgatcccaa
    14701 gctcgatgtg gttgccgtgg tccgactcgc gccagcgcac gagcgtctcc gggtcgtcgg
    14761 caaaccagtc gatccggtcg cccaggttcc agatccccac gtggttgatc cagccgccga
    14821 ggttcgtcga cgacgcgacg tcgggcgaca cggtgaccat ccgcgccgcc acctcgggcg
    14881 ccgactgcgt caaatcgaag aagagccgcc cgagcgcctt ctgagtcgac tccgttccga
    14941 catgctcgcg ccccagcgag tcaggcaccg gaggcggctc accaacctcc accgcgggcc
    15001 gctccaaccg ccgcgccgtg tccgccagca gctctccctc atcgctgtca ataggaaaac
    15061 cggcccacgg gtcatcgaga tcaacgccca gttcgcccgc gagctgctcc atctgcccgg
    15121 gcgtgagcag ggccgagtga ttcgacggat gcccctccgt cgccagccgc cagcccttga
    15181 tcgtgtacgc gaacacgatc gacggccggt cgcgcaccgc gtccgcgtcg cggtagccct
    15241 cgagcagcgc cccgaggtcg tgcccgccga gatcgcggaa cgccgcgatc agctccgagt
    15301 cgtcgagctg gccgaccagc ttcgtgagct cacgccgccc gcgcccgcga cccggtagcc
    15361 gctcgcgtag gtcggaagcc ggcagccgca gcagccgctg gtactcctcg ttgctcatct
    15421 cgtcgatccg ccgctcgagc gcgcgcccgc cggaacgctc gaacagggcg cgcaggttcg
    15481 agccgtactt caccgtgacc gcgtgccagc cggccgcctc gaacatcgac cgaagccgcc
    15541 cggcggcgat gtccggaacc acccggtcga gcgactggcg gttcaggtcc accacccaca
    15601 tcacctcgcc gagccgcgcc accatcgggt cggcgatggc ctcccagacg gcgccctcgt
    15661 cgagttccgc gtcgcctatc agcgcgatct ggcgtccgcc gatcggaacc tcgaagtgcc
    15721 cggccacgta gcggtgcgcc agcgcccccc aaacgggcgc cgtcgcgccg atgccgaccg
    15781 agccggtcga gtagtcgacc gggtccggat ccttcacccg gctcggatag ctctgcagcc
    15841 cctcgaactc gcgcagcgtc gtcaaatatc gccggtcgag ccgcccgagc aggtagttga
    15901 tcgcgtgcag gactggggag gcgtgcggct tcacgctcac ccggtccggc gcctcgagca
    15961 gcgagaacca gagcgcggtc atgatcgaga ccatcgacgc gctcgaggtt tggtggccgc
    16021 ccaccttcac gcccgagcgg ttcggacgca cgcgattcgc ctggtggacc atcgcagtcg
    16081 cgagccacag gacccgccgc tcgatcgacc tgagcgtgtc tagatccggc gcctcgcgcg
    16141 ccgccggcgc ggtctccgtg gaagccacgc gccacacagt aacccggttg agccgcccac
    16201 cggcaggcgt atgatcggag cgagagccga atcggaggag gaggttgcga tgtcgcggct
    16261 gatccggatg gatgcgacgg ggcactcgaa gctcgcggag tggacggcgg gcgacgaggc
    16321 gagcgtggag gctgccgtcg aggcgttccg ccgcgagctc gacgagggct acgtcgcggt
    16381 cgtgcccgag ggcgaagggg aggccacgca ggtgcgcgag ctgccgctcg acgcccacct
    16441 cgtgatcctg cggcgcccga tcgcgggcgg ctgatcccgg catgacacag gcgagcgagg
    16501 cggccggcgc gccgctgcaa gagctcgcgt cagtaccgtg gcgtgagctt
//
LOCUS       DASVXS010000121         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_386812, whole genome shotgun sequence.
ACCESSION   DASVXS010000121 DASVXS010000000
VERSION     DASVXS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386812"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VF752_06635"
     CDS             complement(<1..796)
                     /locus_tag="VF752_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855261.1"
                     /translation="MTPPTGSRPSHDRELARRRLAMLAGVALVSLVAGVVVGARHVSS
                     DQRTGERFVDAWVHGDYGAMWRLLDSDSRKHTRAARFSAAYEHARRLATVERYDRDGD
                     AEDRDGLVEIPLLVTTHSFGTLRLQLRLPISDGHVSWSRNLTFPGVARGEALRRSLRA
                     PSRASILSRDGKVLAEGPAEARSSPLGSTASAIAGSLGPPETPSERAYLYEHGFAPGA
                     PIGRSGLERAFDERLRGTPGGQLYAGDTLLATSSPRPAPKLRTTIDT"
     gene            871..2019
                     /locus_tag="VF752_06640"
     CDS             871..2019
                     /locus_tag="VF752_06640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX8855262.1"
                     /translation="MTAVDWIIIGFTLLMAVWGYAQGLIVGALSLAGFVGGAFLGSRL
                     APLLLTQGSHSPYAPLVSLVAALALGALFATGLEMVGFHLRRQLGPTLGALDGAGGAV
                     LVAAVGLGIAWIVGAVALQTPGARNLRSDIQRSKILTALNDALPPSGPLLNALARFDP
                     LPSIRGPLADVPAPNSRVARDPDVRRAGASVVKILGTACGLGVEGSGWVAGDGMVVTN
                     AHVVAGQTDTSVQLQGQGTRNSARAIWFDPGNDLAVLRVDGLASAPALRLNTNARPGT
                     SGAVLGFPENGPFNVQPARLGPTAMVASEDAYGRGPVQRSITSFRALVRPGNSGGPLV
                     DSDGRVLTTVFAAAVGDREERGFGVPDSVVASALRRAGDPVSTGPCAR"
     gene            2074..>3014
                     /locus_tag="VF752_06645"
     CDS             2074..>3014
                     /locus_tag="VF752_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855263.1"
                     /translation="MTFSLKTVAAAAVAAALIPAGSAFAHRPDSERTTYLLSRSMNGS
                     IPNGPSRNAAVSHDQRIARVIAFESDGSDLTSGDSNNATDVFVVRRAAPWGHNGTFWT
                     MGATTLVSKGLGGQPANGRSYRPGVDGDSHHPPSCVAFVSDASNLVPGDTNGKPDAFV
                     YNLNSGKIVRVSVDSAGKQSNGSAYEVSVDGACERAAFVSDATNLALKGTKNDAWKSA
                     RTSGAKPGVKQVYVHVIKGSKLDAGFKGLTFLASASAKGKAGNADSFDADFARAGKDV
                     VFASNASNLTKGDGNGTSDVYERSFDRKYMHVGHGKGV"
BASE COUNT          417 a         1054 c         1091 g          452 t
ORIGIN      
        1 tcgtgtcgat cgtggtgcgg agcttgggcg ccgggcgcgg cgacgaggtc gcgagcagcg
       61 tgtcgcccgc gtagagctgg ccgcccggcg tgccgcgcag gcgctcgtcg aaggcccgct
      121 cgagacccga gcggccgatc ggggcgccag gcgcgaagcc gtgctcgtac aggtaggcgc
      181 gctcgctcgg agtctcgggc gggccgagcg agcccgcgat cgcgctcgca gtcgagccga
      241 gcggtgagct gcgcgcctcg gctggtccct cggccagcac cttgccgtcg cgggagagga
      301 tgctcgcccg cgacggtgcg cggagcgagc gccgtagcgc ctcgcccctc gctacgcctg
      361 ggaaggtgag gtttcgggac cagctcacgt gtccgtcgct tatcgggagg cgtagctgca
      421 ggcgcagtgt cccgaaggag tgcgtggtca cgagcagcgg gatctcgacc agaccgtcgc
      481 ggtcctccgc gtcgccgtcg cggtcgtagc gctccaccgt ggccagccgg cgggcatgct
      541 cgtaggcagc cgagaagcgc gccgcgcggg tgtgcttgcg cgagtcggag tcgagcagcc
      601 gccacatggc gccgtagtcg ccgtgcaccc acgcgtctac gaatcgctcg ccggtgcgct
      661 gatcggagga gacgtgccgg gcgccgacca cgacaccggc gacgagcgag acgagcgcga
      721 ccccggccag cattgcaagc cgccggcggg cgagttcccg gtcgtggctc gggcgcgatc
      781 cggtcggcgg cgtcatcact cgcgccgagg aggatgcccg ccttccgccc ggctcagtca
      841 tggcaggccg ccgctagtgt ctccgccttc ttgacggcgg tcgattggat catcatcggc
      901 tttacgctgc tgatggcggt gtggggctac gcacagggcc tcatcgtcgg cgcgctatcg
      961 ctggccgggt tcgtgggcgg tgcgtttcta ggttcgcggc tcgctccgct cctcctcacc
     1021 cagggctcgc attctccgta cgcgccgctc gtgtcgctcg tggccgccct cgcgctggga
     1081 gccctgttcg cgacgggcct cgagatggtc ggcttccacc tgcgccgcca gctggggccg
     1141 acgctcggcg cgctcgatgg cgcgggcggc gcggtgctcg tggcggcggt gggactcggc
     1201 atcgcgtgga tcgtcggggc cgtggcgctg cagacgcccg gcgcgcgcaa cttgcggtcg
     1261 gacatccagc gctcgaagat cctgacggcg ctcaacgacg cgctcccgcc gtcgggcccg
     1321 ctgctgaacg cgcttgctcg cttcgaccca ctgccgagca tccgcgggcc gctcgccgac
     1381 gtcccggcgc cgaactcgcg cgttgcgcgc gatccggacg tgcgccgcgc cggagccagc
     1441 gtggtgaaga ttctcggcac ggcctgtggc ctcggggtcg agggctctgg ctgggttgcc
     1501 ggcgacggga tggtggtcac gaacgcacac gtggtggcgg gccagaccga cacgtcggtg
     1561 cagcttcagg gccagggcac gcgcaattcg gcgcgcgcga tctggttcga cccggggaac
     1621 gacctggcgg tcctgagggt ggacggcctg gcgagcgcgc cggcgctgcg gctgaacacg
     1681 aacgcccgcc ccggaacgag cggcgctgtg ctcggctttc ccgagaacgg cccgttcaac
     1741 gtgcagcccg cgcgcctcgg accgacggcg atggtcgcga gcgaggacgc gtacggacgc
     1801 ggaccggtgc agcgcagcat cacgagcttt cgcgcgctcg tgcggcccgg caactcgggc
     1861 ggaccgctcg ttgactcgga cggcagggtc ctgacgaccg ttttcgcggc cgcagtgggc
     1921 gatcgcgagg aacgcggctt cggtgtgccc gattccgtgg tcgcgagtgc tttacgacgc
     1981 gctggggacc cggtttccac tgggccctgc gcacgttgac gagcgtagac gcgccaccct
     2041 caagtggggt acgggagtgc cgatgagagg ggcatgacct tctcgctgaa gacggtggct
     2101 gctgctgcgg tcgcagcagc tctgatcccg gccggcagcg ccttcgccca ccgccccgac
     2161 tccgagcgca ccacctacct gctctctcgc tcgatgaacg gttctatccc gaacgggccc
     2221 tcgcgcaacg ctgcggtctc tcacgaccag cgcatcgcgc gcgtgatcgc gttcgagtcc
     2281 gacggctccg acctgacctc gggcgacagc aacaacgcga ccgacgtctt cgtcgtccgg
     2341 cgcgcagccc catggggcca caacggcacg ttctggacca tgggcgcgac gacgctcgtg
     2401 tcgaaggggc tcggtggcca gcccgccaac ggccgctcct accgccccgg ggttgacggc
     2461 gactcgcacc acccgccgag ctgtgtggcg ttcgtctccg acgcctcgaa cctcgtcccc
     2521 ggcgacacga acggcaagcc cgacgcattc gtctacaacc tgaacagcgg caagatcgtg
     2581 cgcgtctcgg tcgactccgc cggcaagcag tcgaacggct cggcctacga ggtctcggtc
     2641 gacggcgcct gcgagcgtgc cgccttcgtt tcggacgcga ccaacctcgc gctcaagggc
     2701 acgaagaacg acgcctggaa gagcgcgcgg acgagcggcg cgaagcccgg cgtcaagcag
     2761 gtgtacgtgc acgtgatcaa gggctcgaag ctcgacgccg gcttcaaggg cctcaccttc
     2821 ctggcctctg catcggccaa gggcaaggcg ggcaacgcgg acagcttcga cgccgacttc
     2881 gcgcgggcgg gcaaggacgt ggtgttcgcc tcgaatgcgt ccaacctcac gaagggcgac
     2941 ggcaacggca cctccgacgt gtacgagcgg agcttcgacc gcaagtacat gcacgtgggt
     3001 cacggcaagg gcgt
//
LOCUS       DASVXS010000122         8092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1164705, whole genome shotgun sequence.
ACCESSION   DASVXS010000122 DASVXS010000000
VERSION     DASVXS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164705"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VF752_06650"
     CDS             <1..768
                     /locus_tag="VF752_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855264.1"
                     /translation="ELLRFFRNRLRLVISLVQPVLFLFVLGGGLANLLPSSGDVDYRT
                     FIFPGAIALTVLFTALFSAMSIVWDREFGFLREMLVAPVRRGAIVAGKCAGGASIATL
                     QGAVMLLFAGLVHVPYSPVLLLTLLGEMALAAVALTAAGMMLAGHVTKIESFQAVVQF
                     LVLPMFFLSGAVYPVSDLPGWLSALNKVDPVSYAVDPMRRAVFDHITVPDRLEARLNP
                     GISWGDWRLPVAFELLLLALFGAAMLIVAIRQFNRIE"
     gene            801..1187
                     /locus_tag="VF752_06655"
     CDS             801..1187
                     /locus_tag="VF752_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855265.1"
                     /translation="MAVAIDPVAVEEEAARLEIAAIARFLQETLGQQLTAYLAGLRDP
                     KMVGRWASGKVQPRELAKAKLRTAFQAARMIVEAYGVETARAWFVGSNTRLDDEAPAW
                     LLRHAKGIDDLRFVVPTARAFAGAGD"
     gene            1103..1834
                     /locus_tag="VF752_06660"
     CDS             1103..1834
                     /locus_tag="VF752_06660"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEX8855266.1"
                     /translation="MAAPSREGHRRPAVRGPHRPRFRRGRRLSTPSHAWRLGRREAPL
                     EFVPRLYTSWQHRFDDPRREYRTLYCAERADTCLREVLADLRPNAKVLAEMSQLFGRS
                     VAIESAAGTVPREWRDAHVLRRGRLEARGELADIDSVRLRRRLEREHAGLLAAHRMDH
                     LDISEVRSRTRPVTQAISRTLYEDGAAGVRFRSNLDDRPCFALFEGRARLAAERASRD
                     TLVRLRSDEPMLMRVCDELGLTLPA"
     gene            complement(1831..2535)
                     /locus_tag="VF752_06665"
     CDS             complement(1831..2535)
                     /locus_tag="VF752_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX8855267.1"
                     /translation="MKAAQSISDWEVALRLALSLVLCALVGAEREARGQVAGLRTHVI
                     VGMGSTLFTLVSAYAFEDFLVAGPGAAAQRVDPTRIAAQIVSGIGFLGAGAIIRQGAN
                     VRGLTTAAALWIAAAIGMAVGAGYYFGAFIATALVLLSLVGLRYVRPAVIEQFGPPRQ
                     VVMLELEVDPGAERGVGGALERLLDEGADIEAVRSEELGRYSVTLRLPRGASPDELLS
                     DLASANVVRAARIVPS"
     gene            2600..3061
                     /locus_tag="VF752_06670"
     CDS             2600..3061
                     /locus_tag="VF752_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8855268.1"
                     /translation="MTITLEKDAKAARPRGEELPARLRLAITRTARRLRQEADAGLSP
                     SLTAVLATIERSGPVSPSRLAELERIQRPTATRLLARLEERGLVTREADAQDRRCSQV
                     AITAEGRSLLKKLRSRKNAYLARRLKRLEEDDLRTLDRAADILERLLEEGA"
     gene            3058..4437
                     /locus_tag="VF752_06675"
     CDS             3058..4437
                     /locus_tag="VF752_06675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855269.1"
                     /translation="MRAAVTPRLKRTFDALGVPNYRRYFVGQVVSLSGNWMQTVAELW
                     LVLSLTGSALAVGVTTALQFTPMLLVGAFGGVIADRHDKRRLLMLTQVLMALPALALF
                     GLTAGGLVEPWMIFALVFARGAVNAFDNPARQSFLVELVGSNKLINAVGLNSALVQTA
                     RVFGPAGAGIAIATLSVQACFLLNALSFGAMLWALERMRPAELERSEPAPRKRGQLRS
                     ALRYVQSTPQLLIPLSMMALAGTLAYNFQVLLPLMARFSFHGGPGTYAALTSAMGAGA
                     IVGALAVGTRKRVPPAFLSGAALVFGGALAVAALAPTLDLELIALALTGLASVTFAAS
                     VNSALQLGAKPSMRGRVMSLYSVVFLGSTPIGGPLMSLIAGHAGPRTSMLVGAAAALT
                     AGLVARIAYRRTLGITGEGVALPSPGGIPGEEPVLAEAAEAETRRRRVWRPRGEHTGP
                     RPRQASPLG"
     gene            complement(4434..4712)
                     /locus_tag="VF752_06680"
     CDS             complement(4434..4712)
                     /locus_tag="VF752_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HEX8855270.1"
                     /translation="MHVRRATQRLLQLATFGLLVAAVGQELRKPPRKRKWRGTVAGVV
                     PYDLRFPPTVDRVKHALWNPKGSVLQPPAFGVGWTFNFARLAAKLRVG"
     gene            complement(4714..5220)
                     /locus_tag="VF752_06685"
     CDS             complement(4714..5220)
                     /locus_tag="VF752_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8855271.1"
                     /translation="MTTTPAHGILADERKSEREELLGLLEKAYWMEIETVMSYIANSI
                     NPDGIRAQEIKESLDEDIQEELGHAQQFGQRIKELYGTVPGSLEFTAEQNYLQPPEQQ
                     VDIVHVIRGVIEAETGAIEHYNRIIEFCEGTDPVTQDMVIQILHDEEGHRRLFEGFLR
                     ECEAEGLA"
     gene            complement(5262..5957)
                     /locus_tag="VF752_06690"
     CDS             complement(5262..5957)
                     /locus_tag="VF752_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8855272.1"
                     /translation="MNSLPGVLVAGGTGALGTAVVRELLDSGYPVTATWVIEGERDRV
                     REELADRESLSLVEADLLDPAVADAVVAGVEGLGAVVNLVGGYAETGHVHETNPDEFD
                     RLLALNLRPLFLLARAAMPRLAEAGGGSFVGVSARPALRPFPGATAYATAKAAVLALI
                     QALDVEWKARGVRANAVLPSVIDTPANRRSQPDADYSKWVQPSEIAKVIRFLVSDDSA
                     VTSGAAIPVYGRA"
     gene            6010..7002
                     /locus_tag="VF752_06695"
     CDS             6010..7002
                     /locus_tag="VF752_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8855273.1"
                     /translation="MEQPTLLNEKLFAKDLTDGQAVDTVFVVRERTRRSKKNGEAFLK
                     LRLGDVTGSIEAVVWDNVDEIDCVAALGAHVRAAGRFSVDQRFGACLTVRSLRAAHES
                     EYDRSELEEAAPVSFDQMVADLRDLVSTMQDVHLRELLGRCFREGSAIWSRWSTAPAA
                     KRYHQAYRHGLLEHCLSVAQGVSALSATFPGIDRDVAVAGALLHDIGKIDAYESVNGA
                     IDLSDSGKLQGEIALGYYTVRRMIEDIPGFPEETAAALLHIILSHHGQLEHGSPVVPC
                     TREATLVHMVDNLGGKLGSFDRIEKSLSDGERWSGFDRALSGSAYFASPREAAA"
     gene            7086..>8092
                     /locus_tag="VF752_06700"
     CDS             7086..>8092
                     /locus_tag="VF752_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8855274.1"
                     /translation="MAKDTEKLIRQLSLISFLMAERRPVTALEIKREVEGYSGMNDDA
                     FARRFYADRAELESLGIELKVDKPAEGYYEAENYTLPPENFYLPPIEFSDSELAALRT
                     ALSLLDGQFAYAEPLRLALQQLSWGKPSPLDTSERGAIALFEHAGASGRELSQRLSKI
                     ETAIFRRKTILFDYYTMGRGEQGKRKVDPYHLYFEDGQFYLVGYSHERDDIRVFRLSR
                     IRGKVGYSTKAEHDFAKPEDFDPRKYVGRGSWQLGDTLGTARIWLSPRIDWQIDRRDG
                     RRGRMLDASQVEDAPSQGSVFETEYADSGRMVGWALALGDRARILSPPELVEEAHERA
                     RL"
BASE COUNT         1191 a         2888 c         2732 g         1281 t
ORIGIN      
        1 gagctgctgc gcttcttccg caatcgcctg cgcctcgtca tctcgctggt gcagccggtc
       61 ctgttcctct tcgtgctcgg tggcgggctc gccaacctgc tgccctcgag cggcgacgtg
      121 gactaccgca cgttcatctt cccgggagcg atagcgctga ccgtgctgtt caccgcgctc
      181 ttctcggcga tgtcgatcgt gtgggaccgg gagttcggct tcctgcgcga gatgctggtt
      241 gcgcccgtcc gccgcggcgc gatcgtcgcc ggcaaatgcg cgggcggcgc gagcatcgcc
      301 accctgcagg gcgcggtgat gctgctgttc gccggcctcg tgcacgttcc gtactcgccg
      361 gtgctgctgc tcacgctgct cggggagatg gcgctcgcgg ccgtcgccct caccgccgcc
      421 ggcatgatgc tggcggggca cgtgaccaag atcgagtcat tccaggccgt cgtgcagttc
      481 ctggtcctgc ccatgttctt cctctccggc gcggtgtatc cggtgagcga cctgcccggc
      541 tggctctccg ccctcaacaa ggtcgatccg gtgagctatg ccgtcgaccc catgcgccgc
      601 gctgtcttcg accacatcac cgtgccggac cgcctcgagg ccaggctcaa tccaggcatc
      661 tcctggggcg attggcgcct tccggtcgcg ttcgagctgc tcctgctcgc actgttcggg
      721 gccgcgatgc tcattgtcgc gatcaggcag ttcaaccgga tcgagtaggg tgggatagcc
      781 ggtagacttg ccggtggacc atggcagtcg caattgaccc agtcgcagtc gaggaggaag
      841 ccgcccgcct ggagatcgcg gccatcgcgc gcttcctgca ggagacgctt ggccagcagc
      901 tgaccgctta tctcgcggga ctgcgcgacc cgaagatggt gggccgctgg gcatccggca
      961 aggtccagcc ccgcgagctc gcaaaggcca agctccgtac agcgttccaa gcggcgcgga
     1021 tgatcgtcga ggcctacggc gtcgagacgg cgagggcctg gttcgtcggc tcgaacacgc
     1081 gcctggacga cgaggccccc gcgtggctgc tccgtcacgc gaagggcatc gacgacctgc
     1141 ggttcgtggt ccccaccgcc cgcgctttcg ccggggccgg agactgagca caccgagcca
     1201 cgcctggcgg ctcggccgtc gggaggcgcc gctcgagttc gtgccgcggc tctacacgag
     1261 ctggcagcac cggttcgacg acccgcggcg cgagtaccgc acgctctact gcgctgagcg
     1321 cgccgacacc tgcctgcgcg aagtcctcgc cgacctgcgc ccgaacgcga aggtgctcgc
     1381 tgagatgagc cagttgttcg gccgctcggt cgccatcgag agcgctgccg gcaccgtccc
     1441 gcgcgaatgg cgcgacgctc atgtgctgcg gcgcgggcgg ctcgaggcgc gcggcgagct
     1501 cgccgacatc gactcggtgc ggctgcggcg ccgcctggag cgcgagcacg ccgggttgct
     1561 ggcggcccac cgcatggacc acctcgacat cagcgaggta cgcagccgca cgcggcctgt
     1621 cacccaggcg atttcgcgga cgctctacga ggatggcgcc gcgggcgtgc gcttcaggtc
     1681 caacctcgac gaccggccgt gcttcgcgct gttcgagggc cgcgctcggc tggcggcgga
     1741 gcgcgcctcg cgcgacaccc tggtgcgcct tcgttccgac gagccgatgc tcatgcgggt
     1801 gtgcgacgag ctcggcctca cgctgccggc ctagctcggg acgatccgtg ccgcgcgcac
     1861 gacgttcgca gaggcgaggt ccgacaggag ctcgtccgga gaggctccgc gcggcagccg
     1921 cagcgtcacc gagtagcgtc ccaattcctc cgaccgcacc gcctcgatgt cggcgccctc
     1981 gtcgagcaac cgttcgagcg cgccgcccac gccgcgttcc gccccgggat cgacctcgag
     2041 ctcgagcatc acgacctgcc gcggcggccc gaactgctcg atcaccgctg gacgcacgta
     2101 gcgaagcccg acgagtgaca gcagcaccag cgcggttgcg atgaacgctc cgaagtagta
     2161 ccccgcccca accgccatac cgatcgcggc cgcgatccac agcgccgccg ccgtcgtgag
     2221 cccgcgcacg ttcgcgccct ggcggatgat cgcgcctgca ccgaggaagc cgatgccgga
     2281 cacgatctgc gcggcgatgc gcgtcgggtc gacgcgctgg gcggcggcgc ctggcccggc
     2341 aacgaggaag tcctcgaacg cgtatgccga caccagggtg aagagcgtcg agcccatgcc
     2401 gacgatcacg tgcgtgcgca gcccggccac ctggccgcgc gcctcgcgct cggcgcccac
     2461 cagcgcacac aggaccagcg acagcgccaa tcgcagcgcc acttcccagt cgctgatgct
     2521 ctgagccgcc ttcacaggtg cagcgtacgc cgcattgccg gcgagacgcc ccacatatag
     2581 tttgctaagg taattagcaa tgacaattac tcttgagaag gatgcaaagg cggcccgccc
     2641 gagaggcgag gagctgccgg cgcgccttcg cctggccatc acccgcaccg cccgccgcct
     2701 ccgccaggag gccgacgccg ggctgtcccc ctcgctcacc gccgtactcg cgacgatcga
     2761 gcgcagcggc cccgtctcac ccagccgcct cgccgagctc gagcgcatcc agcgccccac
     2821 agccacccgg ctgctcgctc ggctcgagga gcgcgggctc gtgacacgcg aggccgacgc
     2881 gcaggatcgg cgctgcagtc aagtggcgat cacggccgag ggccgctcgc tcctgaagaa
     2941 gcttcgctcg cgcaagaacg cctacctcgc acggcgcttg aagcgcctcg aggaggacga
     3001 cctgcggacg ctcgaccgcg cggcggacat tctcgagcgg ctgctggagg agggcgcttg
     3061 agggcggccg tgacgccgcg gctcaagcgg accttcgacg cactcggcgt acccaactac
     3121 cggcgctatt tcgtcggcca ggtcgtctcg ctcagcggca actggatgca gacggtcgcg
     3181 gagctctggc tcgtgctctc gctcacgggg agtgcgctcg cagtcggcgt gaccacggcg
     3241 ctgcagttca ccccgatgct gctcgtgggt gcgttcggcg gagtgatcgc cgaccgccac
     3301 gacaagcggc ggctcctgat gctcacgcag gtcctgatgg ctctgcccgc cttggcgttg
     3361 ttcggcctca cggctggcgg cctggtcgag ccgtggatga tcttcgcgct cgtgttcgcg
     3421 cgcggcgccg tgaacgcgtt cgacaatccc gcgcgccaga gcttcctggt ggagctcgtc
     3481 ggtagcaaca agctgatcaa cgcagtcggg ctcaacagcg cgctcgtgca gaccgcccga
     3541 gtgttcggcc ccgcgggggc gggcatcgcg atcgcgacgc tcagcgtgca ggcgtgcttc
     3601 ctcctcaacg cgctctcgtt cggcgcgatg ctctgggcgc tcgagcggat gcggcccgct
     3661 gagctggaac gcagcgagcc ggcgccgcgc aagcgcggcc agctgcgctc ggcgcttcgc
     3721 tacgtgcaaa gcaccccgca gctgctgatt ccgctgtcga tgatggccct ggccggcacg
     3781 ttggcctaca acttccaggt gctgctgccg ctgatggcgc gcttctcgtt ccacggcggt
     3841 cccggcacgt acgccgcgct cacctcggcg atgggcgccg gcgcgatcgt gggagccctc
     3901 gccgtcggca cccgaaagcg cgtgccgccg gcgttcctct ccggcgccgc cctcgtgttc
     3961 ggcggggcgc tggccgtcgc agcgctcgcg cccacgctcg acctcgagct gatcgccctg
     4021 gcgctcacgg gcttggcgag cgtgaccttc gccgcgagcg tgaactccgc gctgcagctc
     4081 ggcgccaagc catcgatgcg tggacgggtg atgtcgctct actcggtcgt gttcctcggg
     4141 agcacgccga tcggcgggcc gctgatgagc ctgatcgcgg gccacgcggg cccgcgcacc
     4201 tcaatgctgg tcggcgccgc agccgcattg accgccgggc tggttgcccg catcgcgtac
     4261 cgccgcacgc tggggatcac aggcgagggc gtcgcactgc catcgccggg cggaatcccg
     4321 ggcgaggagc cggtgttggc ggaggcggcc gaggccgaaa cccggcgcag gcgcgtttgg
     4381 cgcccacgtg gggagcacac gggcccgcgc ccgcgtcagg cttcgccgct cggctagccg
     4441 acgcgtagct tcgctgcgag gcgagcgaaa ttgaacgtcc agccgacgcc gaaggccggc
     4501 ggctgcagca cggatccctt ggggttccaa agcgcgtgct tcacgcggtc cacggtcggc
     4561 gggaagcgca ggtcgtaggg aacgacgccg gctactgtgc cgcgccactt gcgcttgcgg
     4621 ggaggtttgc gcagctcttg gccgacggca gcgaccagaa gtccgaacgt cgcgagctgc
     4681 agcagccgct gcgttgccct tcggacgtgc aactcaggca agtccctcgg cttcgcactc
     4741 gcgcaggaag ccttcgaaca gccgccggtg gccttcctcg tcgtggagga tctggatgac
     4801 catgtcctgc gtaaccgggt ccgttccttc gcagaactcg atgatccggt tgtagtgctc
     4861 gatggcgccg gtctcggcct cgatcactcc gcggatcacg tggacgatgt ccacctgctg
     4921 ctcggggggc tgcaggtagt tctgctcggc ggtgaattcg agtgagccag gaaccgtccc
     4981 gtagagctcc ttgattcgct gcccgaactg ctgagcgtgt ccgagctcct cctggatgtc
     5041 ctcgtcgagg gactccttga tctcctgagc gcggatgccg tccgggttga tggagttggc
     5101 gatgtagctc atcaccgtct cgatctccat ccagtacgcc ttctcgagca gcccgagcag
     5161 ctcttcgcgc tcggacttgc gttcgtcggc caggatgccg tgtgcaggtg tcgttgtcat
     5221 ggaggcctgc tttcccgcgc gccccgcagg cgaaacgacc gctatgcgcg gccgtagacg
     5281 gggatcgcgg cgccgctcgt gacggccgag tcgtccgaca cgaggaagcg gatgaccttg
     5341 gcgatctcgc tcggctggac ccactttgag tagtcggcgt caggctggct gcgcctgttc
     5401 gccggcgtgt cgatcacgct cgggagcacg gcgttcgcgc ggaccccgcg cgctttccac
     5461 tcgacgtcga gcgcctggat gagcgcaagc accgccgcct tcgccgtcgc gtaagccgtg
     5521 gcgccgggga acggccggag cgccggacgc gccgacacgc ccacgaatga tccacctcca
     5581 gcttcggcca ggcgcggcat cgcggcacgc gccagcagga acagcggcct caggttcaac
     5641 gcgagcagcc ggtcgaattc gtcagggttg gtctcgtgca cgtgccccgt ctcggcatag
     5701 ccccctacga gattcacgac cgcccccaac ccctccacgc cggccacaac agcgtcagct
     5761 accgccggat ccaggaggtc ggcctcgacg aggctcaggg actcgcggtc cgcgagctcc
     5821 tctctgaccc ggtcgcgctc cccctcgatc acccaggtcg ccgtgaccgg gtagccgcta
     5881 tcgaggagct ccctcacgac ggcggtgcca agagctccgg tgccgcccgc gacgagcact
     5941 cccgggagcg agttcatcgt gtggaactta gccgttcggt ccggtcccgg gtctaagttg
     6001 gaccgcctta tggagcagcc aactctcctc aacgagaagc tgttcgcgaa ggacctgacg
     6061 gacgggcagg ccgtggacac ggtgttcgtc gtgcgggagc gcacccggcg ttcgaagaag
     6121 aacggggagg cgttcctcaa gctgcggctc ggcgacgtga cgggctcgat cgaggccgtg
     6181 gtctgggaca acgtcgacga gatcgactgc gtggcggccc tcggcgccca cgtccgggcg
     6241 gcggggcgct tctcggtcga ccagcgcttc ggcgcgtgtc tcacggtgcg ctcgctgcgc
     6301 gcagcgcacg agtcggagta cgaccgctcg gagctcgagg aggcggcgcc cgtgtccttc
     6361 gatcagatgg ttgcggacct gcgcgacctg gtgtcgacga tgcaggacgt tcacctccgc
     6421 gagctcctcg ggcggtgctt tcgggagggc tcggcgatat ggagccggtg gagcaccgcg
     6481 ccggcggcca agcgctacca ccaggcctac aggcacgggc tgctcgagca ctgcttgtcc
     6541 gtcgcgcagg gcgtcagcgc gctgtcggcc accttcccgg gcatcgaccg cgacgtcgcc
     6601 gtggctggtg cgctcctgca cgacatcggc aagatcgacg cctacgaatc ggtgaacggc
     6661 gcgatcgacc tgagcgacag cggcaagctc cagggtgaga tcgcgctcgg ctactacacg
     6721 gtccgccgca tgatcgagga catcccaggc ttccccgagg aaacagctgc agcgctgctc
     6781 cacatcatcc tctcgcacca cggccagctc gagcacggca gcccggtcgt cccgtgcacg
     6841 cgcgaggcca cgctcgtgca catggtcgac aacctcggcg gcaagctcgg cagcttcgac
     6901 cgcatcgaga agtcgctcag cgacggggag cgctggtcag gcttcgaccg agcgctctcc
     6961 ggctccgcct atttcgcatc accgcgcgaa gcggcggcct aagccgcaaa cagcgaagat
     7021 cgcctgcagg ggctggcgca accgatgccg gtgcttctct ctttgcacgc ttatcctgct
     7081 tgcggatggc caaggacacc gaaaagctca tccggcagct ctcgctgatc tcattcctga
     7141 tggcagagcg ccgccccgtc acggcgctcg agatcaagcg cgaggtcgag ggctactcgg
     7201 gcatgaacga cgacgcgttc gcccggcgct tctacgccga ccgcgcagag ctcgagtcgc
     7261 tcgggatcga gctgaaggtc gacaagcccg ccgaggggta ctacgaggcc gagaactaca
     7321 cgctcccgcc cgagaatttc taccttccgc cgattgagtt ctcggactcc gagcttgcgg
     7381 cgctgcgcac cgcgctgagc ctgctcgacg ggcagttcgc gtacgccgag ccgctgcgcc
     7441 tcgcgctcca gcagctctcc tggggcaagc cctcgccgct cgacacctcc gagcgcggcg
     7501 ccatcgccct cttcgagcac gccggcgcga gcgggcgcga gctgtcgcag aggctgtcga
     7561 agatcgagac ggcgatcttc cggcgcaaga cgatcctttt cgactactac acgatgggcc
     7621 gcggcgagca gggcaagcgc aaggtcgacc cgtaccacct ctacttcgag gacggccagt
     7681 tctacctcgt cggctactca cacgagcgcg acgacatccg cgtgttccgg ctctcccgga
     7741 tcaggggcaa ggtcggctac tccacgaagg cggagcacga cttcgccaag ccagaggact
     7801 tcgacccgcg caagtacgtg ggccgcggca gctggcagct gggcgacacc ctcggaaccg
     7861 cgcgcatatg gctctcgccg cggatcgact ggcagatcga ccgccgcgac ggccgccgcg
     7921 gtcgcatgct cgacgcctcg caggtcgagg acgcgccgag ccagggcagc gtgttcgaga
     7981 ccgagtacgc cgactcgggc cgcatggtcg gctgggccct ggcgctcggc gaccgcgccc
     8041 gcatcctctc cccgcccgag ctggtggagg aggcccacga gcgcgcgcgg ct
//
LOCUS       DASVXS010000123         7895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_582214, whole genome shotgun sequence.
ACCESSION   DASVXS010000123 DASVXS010000000
VERSION     DASVXS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582214"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF752_06705"
     CDS             complement(<1..212)
                     /locus_tag="VF752_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8855275.1"
                     /translation="MAVGTFISVGRSIETAMQRAALADELGYESVYVTHVAGRDSLTV
                     LAAYATRTKRVRLGTGVLPIYSRTPVA"
     gene            251..829
                     /locus_tag="VF752_06710"
     CDS             251..829
                     /locus_tag="VF752_06710"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX8855276.1"
                     /translation="MIGAVLAGGAARRMGGTKPAAELGKRPLISYPVAAMSEVCERVA
                     VVCKASTPLPDLEGVERWDEPAEPTHPLAGIVHALERAGEPVLVCAADMPFVTADALR
                     TIVHAGQRAAGTPAVVAVADGMLQPLLALYAPACLERFRTAEAAARLTDTVEALGPVR
                     VALPGRLVTSVDTPDQLSAAEAELSGRSAPRP"
     gene            complement(780..953)
                     /locus_tag="VF752_06715"
     CDS             complement(780..953)
                     /locus_tag="VF752_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855277.1"
                     /translation="MRILAQLAIVLAAFGVGVGLGELFGAANLGVSFGIGQIAAAFTA
                     AALIVRTARPPRR"
     gene            complement(1034..1480)
                     /locus_tag="VF752_06720"
     CDS             complement(1034..1480)
                     /locus_tag="VF752_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX8855278.1"
                     /translation="MERIGTSAVFNGISLLSGNISITFQVGANDGETIQVTTMTLYGG
                     AVPLSAFQLSPTGANDISEIDAAIINLNAMRATYGATQNRLEHLINNLSAYEENITAS
                     ESRVRDVDMADEMANFTKLQILQQAGTSMLAQANQSSQSVLELLRG"
     gene            1516..1641
                     /locus_tag="VF752_06725"
     CDS             1516..1641
                     /locus_tag="VF752_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855279.1"
                     /translation="MNEDEDKALALQLGAQDYIDKGLAPDALAAALQSAISQAGA"
     gene            complement(1651..2124)
                     /locus_tag="VF752_06730"
     CDS             complement(1651..2124)
                     /locus_tag="VF752_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8855280.1"
                     /translation="MDGFRELTIGEVARRAGVATSSIRCYERIGLLPQPERTHGQRRY
                     GTEVLGKLGFIGVAQSAGFKLREIKELIDGVDSADGMGEQMRSLSSRKLEEVEALLER
                     TKAMKGWLETAKQCGCASPTECALFPVPGEEAADADWSLRVVQVKGDDCRRPPAA"
     gene            2209..2580
                     /locus_tag="VF752_06735"
     CDS             2209..2580
                     /locus_tag="VF752_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855281.1"
                     /translation="MTVKTHTSLDLDALKRGYEEWDIDGLLELYADDVELTQVDRDNP
                     PSSPRVRHGKEVFKGMFDHCAAAGVTVTVENLVADENRAAATITCEFPGGRKVVANSI
                     LEIEDGRIVRERDVQAGDPKQ"
     gene            2650..3027
                     /locus_tag="VF752_06740"
     CDS             2650..3027
                     /locus_tag="VF752_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855282.1"
                     /translation="MPKASKQTASEHESVEGYEGHFEHFDGGWTVGFETYTQDADLAP
                     FFKGLPNDQCQCEHMGYVIKGKVAFRSGDTEEVFEAGDAYYAGPGHTPILFAGTEVVE
                     FSPTEELGRTMEVVTKNMQEMEA"
     gene            3059..3469
                     /locus_tag="VF752_06745"
     CDS             3059..3469
                     /locus_tag="VF752_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855283.1"
                     /translation="MAPEPALRIARHTERLDEVVAFYRDGIELSEVGGFRGHDGYDGV
                     FLAVPGTRAHLELTAGGEHGAPAPHPESLLVLYLGEVRAVEAILRRAGVEPVVPANPY
                     WAEHGVTVMDPDGFRVVLVPEWWEPAGNARSDPH"
     gene            3555..4343
                     /locus_tag="VF752_06750"
     CDS             3555..4343
                     /locus_tag="VF752_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8855284.1"
                     /translation="MNATLETHSAVTDEGSRELVVFRLDAGEYALPVANVGEVLRMVA
                     ITPVPEAPEWLPGVINLRGRVIPVIDLRRKLHLPEAKVGVKAPIIVAEHDGAPVPIVI
                     VQHITVGFHQGLADWLNNVTPLTVKLGVNGEPLRAGEVVIAPGEHHMAIDSVKRVKLT
                     TEPAIGGHRPSVTHMFRSVARVYGKHALGVILTGMGDDGAEGLVQVKRAGGWVIGQDE
                     ASCVVYGMPREAAARGAVTEVLPLTDIAGAITQAWSHGRLPVVV"
     gene            4384..6165
                     /locus_tag="VF752_06755"
     CDS             4384..6165
                     /locus_tag="VF752_06755"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX8855285.1"
                     /translation="MADGHILLAVGALLTAGIALSLLAGRLRLPGLLLFLGLGMIVGS
                     EVLGWISFDDYRLARTIGIVALVLILFEGGLAAGWPEVRPVLAPSLSLALFGTLLTAI
                     VSGFIAAALFDFSTLEGLLLGSILAATDAAAIFALLRGSTLRRRLARTLEGESGFNDP
                     VAVLLVLGFVDWITKPDYGVVDMMGLFARQLGIGAVIGLAIGWLAVQGFKRARLATAG
                     LYPVGSMGAAALAFGAADSLHGSGFLAVYLTGLALGGASIPARRTISAFHDGVSWLAQ
                     LGLFFSLGLLVFPSQLGDVAVESVVLAVAIVVLARPLASFLVTLPFRFTNGERLVVGW
                     AGMRGAVPVVLATFPVIDHVPHSLQFFNIAFFVVLISTLVQGVTLEPFARAFGVTTDE
                     PALPRPLAESGTIRRLGAEVLEFPVGSEDAVVGRPVRELGLPRDAVVNVIVRGAEAIP
                     PRGSTRIEAGDRLHVLVRSEFARELESLQNVWRHGPLGGERVDRAMPRGHPAVFTSRR
                     WTDADGDPGRPETIGGVAVAEHLRTRRDMRGALVMLADGRYAVTGPVLASGGRLAVQQ
                     HARRRLSRSASDAERAWWQEVVGALAR"
     gene            6258..6779
                     /locus_tag="VF752_06760"
                     /pseudo
     CDS             6258..6779
                     /locus_tag="VF752_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6728..7063
                     /locus_tag="VF752_06765"
     CDS             6728..7063
                     /locus_tag="VF752_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX8855286.1"
                     /translation="MSTISLGAAIGGNVFSLTATGTADISEIDFAISQVSAQRATFGS
                     VQNRLEHTLNNLSTYQENLVASESRIRDVDMASEMVEFSKLQILQQAGTSMLAQANQA
                     PQSVLSLLR"
     gene            complement(7184..7336)
                     /locus_tag="VF752_06770"
     CDS             complement(7184..7336)
                     /locus_tag="VF752_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855287.1"
                     /translation="MTERPRPDPDHTREQLQRLREELDEPEPEPEPESEPEGEPEEEE
                     GDEDDE"
     gene            complement(7333..7545)
                     /locus_tag="VF752_06775"
     CDS             complement(7333..7545)
                     /locus_tag="VF752_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855288.1"
                     /translation="MIFGALKREGEMGESAIAKEAGLRPEGISSLLERLQHDGHVTRT
                     AHGRWRLTPAEAMHTKPRPHPREPKP"
     gene            7667..>7895
                     /locus_tag="VF752_06780"
     CDS             7667..>7895
                     /locus_tag="VF752_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855289.1"
                     /translation="MSLDTVMARIAELQSAFNAPAAQPSNKSTATASTAFAGALQSAT
                     AAQASGSLTAVPATGSGVGARMVQIAQGEVGQ"
BASE COUNT         1191 a         2667 c         2749 g         1288 t
ORIGIN      
        1 gcgaccggcg tgcgcgagta gatcgggagc accccggtgc ctaggcgcac gcgcttcgtg
       61 cgcgtcgcgt acgcggcgag aacggtgagc gagtcgcgcc cagcgacatg cgtgacgtag
      121 accgactcgt agccgagctc gtccgccagc gccgcccgct gcatcgccgt ctcgatcgag
      181 cgcccgacag agatgaaggt gccgaccgcc aaggctgtgg cagcataacc ggcgtgcgcg
      241 ggagcccctc gtgatcggcg ccgtgctggc cggcggtgcg gcgcggcgca tgggcggcac
      301 aaagcccgcg gcagagctcg gcaaacgccc gctcatctcc tatccagtgg cggcgatgtc
      361 cgaagtgtgc gagcgggtgg ccgtggtgtg caaggcgagc acgccgctgc ccgacctgga
      421 aggcgtcgag cgctgggacg agcccgcgga gccgacgcac ccgctcgccg gcatcgtcca
      481 tgcgctcgag cgggccggcg agccggtgct cgtgtgcgcg gccgacatgc cgttcgtgac
      541 ggccgacgcc ctgcgcacga tcgtccacgc cgggcagcgc gcagcgggca cgccggcggt
      601 ggttgcggtc gccgacggga tgctccagcc gctgctcgcc ctctacgcgc cggcgtgtct
      661 cgagcgcttc cgcacggcgg aggccgctgc ccggctcacg gacacagttg aggcgctcgg
      721 tccggtgcgt gtagcgcttc cgggccggct cgtgacgagc gtcgacacac ccgaccagct
      781 cagcgccgcg gaggccgagc tgtccggacg atcagcgccg cggccgtgaa cgcagcggcg
      841 atctggccga tcccgaacga cacgccgagg ttggccgcgc cgaagagctc accgaggccg
      901 acgccgacgc cgaaggcggc gagcacgatc gcgagctggg cgaggatgcg catggccggc
      961 ggagcataga aagcgaagcg ccccgccggc cgggaaggcc gccggggcgc aagcgctgcg
     1021 agtactccgg gggttagccg cggagcagtt cgagcacgct ctgcgacgac tggttcgcct
     1081 gcgcgagcat cgacgtgccg gcctgctgca ggatctgcag cttcgtgaag ttcgccatct
     1141 cgtcggccat gtccacgtcg cggacacggc tctccgaggc ggtgatgttc tcctcgtagg
     1201 ccgacaggtt gttgatgagg tgctcgaggc gattctgggt cgcgccgtag gtggcacgca
     1261 tcgcgttgag gttgatgatc gcggcgtcga tctccgagat gtcgttcgcg ccggtggggc
     1321 tcagctggaa cgccgagagc ggtacggccc cgccgtacag cgtcatcgtg gtcacctgga
     1381 tcgtctcgcc gtcgttggcg ccgacctgga acgtgatcga gatgttgccg ctcagcaggc
     1441 tgatgccgtt gaacacggcg ctcgtgccga ttcgctccac ctcggaccac ggcgatcgtc
     1501 ctgaacagcg ccactgtgaa cgaggacgag gacaaggctc ttgctctcca gctcggcgct
     1561 caggactaca tcgacaaggg gctcgctccg gacgctctcg cggccgcgct tcaaagcgcg
     1621 atctcgcaag ctggcgcctg actgcccgcc tcacgcggcc ggagggcgac gacagtcgtc
     1681 gcccttcacc tggaccaccc gaagggacca gtcggcgtcc gcagcctcct cgccgggcac
     1741 cgggaagagc gcgcactctg tggggctggc gcagccgcat tgcttcgccg tctcgagcca
     1801 gcccttcatc gcctttgtgc gctcgagcag cgcctcgacc tcctcgagct tccggctcga
     1861 cagcgaacgc atctgctcgc ccatgccgtc ggcgctgtcg acgccgtcga tcagctcctt
     1921 gatctcgcgc aatttgaagc ccgcgctctg cgcgaccccg atgaagccga gcttgccgag
     1981 cacttccgtg ccgtaacgcc gctgcccgtg cgtgcgctcg ggttgaggta gcaagccgat
     2041 gcgctcgtag cagcggatcg aggatgtcgc cacaccggcc cgtcgagcga cctcgccgat
     2101 cgtcagctcc cggaatccgt ccatcgtgga tcaggccctt gacttgaagc gcagttcaag
     2161 ggcgatcgta gtgcgcgcac ggtcacgcac acaaccgagg aggtaacgat gaccgtcaag
     2221 acacacactt cgctcgacct cgatgcgctg aagcgcggtt acgaggagtg ggacatcgac
     2281 ggcttgttgg agctctacgc ggacgacgtg gagctgaccc aggttgaccg cgacaaccct
     2341 ccgagctcgc cgcgcgtgcg gcacggcaag gaggtgttca agggaatgtt cgaccactgc
     2401 gccgcggcgg gtgtcacggt gacggtcgaa aacctcgtcg cggatgagaa ccgcgccgcc
     2461 gccacgatca cctgcgagtt cccgggcggc cgcaaggtcg tcgccaactc gatcctcgag
     2521 atcgaggacg gacggatcgt ccgcgagcgg gacgtccagg cgggtgaccc gaagcagtga
     2581 gacggcggac cgcttgctga tccgcgggca agccgggagt gacaccaagt ccttcaaagg
     2641 aggtacgaga tgccgaaggc atcgaagcag accgcttccg agcacgagtc ggtcgaaggc
     2701 tacgaaggcc acttcgagca cttcgatggt ggctggaccg tgggcttcga gacctacacc
     2761 caggacgccg acctcgcccc gttcttcaag gggcttccga atgatcagtg ccagtgcgag
     2821 cacatgggct acgtgatcaa ggggaaggtc gcgtttcgct ccggagatac agaagaggtc
     2881 ttcgaggccg gcgacgcgta ctacgccggc ccggggcata ccccgattct cttcgcggga
     2941 accgaggtgg tcgagttcag cccgaccgag gaactcggcc ggacgatgga ggtcgtgacc
     3001 aagaacatgc aggagatgga ggcgtaacgg atatacatcc ccagcgcact ggcgcagggt
     3061 ggcacctgaa ccagcactgc ggatagctcg gcacaccgag cgactggacg aggtcgtggc
     3121 gttctaccgc gacggcatcg agctgagcga ggtcggcggc ttccgcgggc acgatggtta
     3181 cgacggcgtg tttctcgcgg tgccgggcac ccgcgcgcat ctcgagctca ccgccggcgg
     3241 cgagcacggc gctccggcgc ctcatcctga gtcgctgctc gtgctctacc tcggtgaggt
     3301 cagggccgtg gaggcaatcc tgcggcgcgc cggcgtcgag ccggttgttc ccgcgaaccc
     3361 ttattgggcg gagcacggtg tgaccgtcat ggatccggat ggcttccgag tcgtgctggt
     3421 gcccgagtgg tgggagcctg ctgggaacgc gaggtccgat ccacactgat cgtgccagga
     3481 ggtggcgtcc gggggcgtcg aacaagcgtc cacccgcgtt ccgcagcccc gctccctgag
     3541 ccgaataccc gggcgtgaac gcaacgctcg aaacccactc tgccgttacc gacgaaggat
     3601 cgcgggagct agtcgtcttc cggctcgacg cgggcgaata cgccctccct gtggccaacg
     3661 tgggcgaggt gctgcgcatg gtcgccatca cgccggtgcc cgaggcgccc gagtggctgc
     3721 cgggcgtgat caaccttcgc ggccgcgtca tcccggtcat cgacctgcgg cggaagcttc
     3781 atctccccga ggcgaaggtc ggcgtgaagg ccccgatcat cgtcgccgag cacgacggcg
     3841 cgcctgtccc gatcgtgatc gtgcagcaca tcaccgtcgg cttccatcag gggcttgccg
     3901 actggctgaa caacgtcacg ccgctcaccg tcaagctcgg cgtgaacggc gagccgctcc
     3961 gcgccggcga ggtcgtgatc gccccgggcg agcaccacat ggcgattgac tcggtgaagc
     4021 gcgtgaagct caccactgag ccggcgattg gcggccaccg gccgtccgtc acgcacatgt
     4081 tccggtcggt cgcacgcgtg tacggcaaac acgcgctcgg cgtgatcctc acgggcatgg
     4141 gcgacgacgg cgccgagggg ctcgtgcagg tgaagcgcgc tggcggctgg gtgatcggcc
     4201 aggacgaggc cagctgcgtg gtgtacggga tgccgcgcga agcggctgcg cgtggagcgg
     4261 tgaccgaggt cttgccactc accgacatcg ccggcgcgat cacccaggcc tggtcgcacg
     4321 ggcgcctccc cgtcgtggtc taaccgcaac gcacacgggg gacctcttga aataagttgg
     4381 cctatggccg acggccacat cctgctcgcg gtaggggcgc tcctcactgc cggcatagcc
     4441 ctgtcgctcc ttgccggccg cctgcgcttg cccggcctgc tcctgttcct cgggttgggg
     4501 atgatcgtcg gctccgaagt cctcggctgg atctcgttcg acgactaccg cctcgcgcgc
     4561 accatcggga tcgtcgcgct ggtgctgatc ctgttcgagg gaggactcgc ggccggctgg
     4621 ccggaggtcc ggccggtgct ggcgccgtcg ctctcgctcg cgctcttcgg gacgctcctc
     4681 accgccatcg tctccggctt catcgctgca gcgctgttcg acttctcgac tctcgagggg
     4741 ctgctgctcg gctcgattct cgccgccacc gatgccgccg cgatcttcgc gctgctgcgc
     4801 ggctctacgc tcagacgccg gctcgcgagg acgctcgagg gcgagtcggg cttcaacgac
     4861 ccggtggcgg tccttctcgt gctgggcttc gtcgactgga tcacgaagcc ggactacggc
     4921 gtggtcgaca tgatggggct gttcgcgcgc cagctcggca tcggcgcggt gataggtctc
     4981 gccatcggct ggctggcggt tcagggcttc aagcgtgcgc ggctcgctac ggccggtctg
     5041 taccccgtgg gctcgatggg cgcggccgca ctcgcgttcg gcgccgccga cagcctgcac
     5101 ggctcgggct tcctggccgt ctacctcacc ggtctcgcgc tcggcggcgc gagtatcccg
     5161 gcgcggcgaa ccatctcggc gttccacgac ggggtgtcgt ggctggctca gctcggcctg
     5221 ttcttcagcc tcggcctgct cgtgttcccg agccagcttg gtgacgtggc ggtcgagtcg
     5281 gtcgtgctcg ccgtcgcgat cgtggtgctg gcgcggccgc tcgcgtcgtt cctggtgacg
     5341 ctgccgttcc ggttcacgaa cggcgagcgg ctggtggtcg ggtgggcggg catgcgcggc
     5401 gcggtacccg tcgtgctcgc gacctttccg gtgatcgatc acgtgccgca cagcctgcag
     5461 ttcttcaaca tcgcgttctt cgtcgtgctc atctccacgc tggttcaggg cgtgacgctc
     5521 gagccgtttg cgcgcgcgtt cggcgtgacg accgacgaac ccgcacttcc gcgccctctc
     5581 gcagagagcg gcacgatccg caggctcgga gcggaggtgc tcgagttccc ggtgggatcc
     5641 gaggacgcgg tagtcggccg gccggtgcgc gagctggggc tccctcgcga tgcggtcgtg
     5701 aacgtgatcg tgcgcggtgc cgaggccata ccgccacgcg gctccactcg aatcgaagcc
     5761 ggcgaccgcc tgcacgtgct cgtgcgctcc gagttcgcac gtgaactcga gagtctgcag
     5821 aacgtgtggc gccacggccc gctcggcggg gagagggtgg accgcgcgat gccgcggggc
     5881 catccggcgg ttttcacgtc acgccgctgg accgacgccg acggcgaccc cggccgcccc
     5941 gagacaatcg gcggcgttgc ggtggcggag cacctgcgga cccgtcgcga catgcgcggc
     6001 gcgctcgtga tgctggccga cgggcgttac gcggtcacgg ggccggtact cgcatctggc
     6061 gggcgtctag cggtgcagca gcacgccagg agacggctgt cacggtcggc gagcgacgcg
     6121 gagcgcgcct ggtggcagga agtcgtcggc gccctggcgc gttgacgcct aaaggcccgc
     6181 cgagcgcggt cgattaccgg gcggaacata gggggctaca tggagtggcc ccggcacgcc
     6241 atcaaggagg atgagaagtg tcactgcgta ttcagcacaa cgtcgagggg ttcaacgccc
     6301 agcgtccgct cgtcgagacg ggcgatcggc tgtcgaagtc gatggagcgt ctgtcgtcgg
     6361 gctatcggat caaccgcgct gcggacgacg ccgccggtct cgcgatcagc gagaagctgc
     6421 gtggccagat caatggtctc gcgcaggcgg gccgcaacgc ccaggacgcc gtttcgctcg
     6481 tgcagacggc tgagggttcg ctgaccgagg tccacgcgat gctgcagcgc gtccgtgagc
     6541 tcgccgtgca gtaaaagaac ggcacgctct cgacgcagga ccgcacggcg atccagtcgg
     6601 aggtcaacca gctcggctcg gagatcgagc ggatcggcaa ggccgccgac ttcaacggga
     6661 tcgacctgct cgctaacgcg aacgcgatca ccttccgggt gggcgcgaac gacagcactc
     6721 gatcacggtg tcgacgatca gcctcggcgc cgcgatcggc ggcaacgtgt tctcgctgac
     6781 cgcgacgggc accgccgaca tctccgagat cgatttcgcg atctcccagg tttcggctca
     6841 gcgtgcgacg ttcggttctg ttcagaaccg gctcgagcac acgctgaaca acctctcgac
     6901 ctaccaggag aacctggtgg cctccgagag ccgtatccgc gacgtggaca tggcctctga
     6961 gatggtcgag ttcagcaagc tgcagatcct gcagcaggcc ggcacgagca tgctcgcgca
     7021 ggccaaccag gcaccgcagt cggtgctttc actgctccgc taaggagtca catagccacc
     7081 ggggacgagc caccccgagg gacggatcgg agtgggcggg ccaagtggcc cgcccgctca
     7141 ttttgttcag tggcgggtcg agggacccgc ccatctcgct ttctcactcg tcgtcctcgt
     7201 cgccctcttc ctcttcgggc tcgccctctg gctcggattc gggctctggc tcgggctccg
     7261 gctcatcgag ctcctcgcgg agtcgctgaa gctgctcgcg agtgtggtcc gggtctggac
     7321 gagggcgctc tgtcacggct tcggctctcg gggatgcggc cgcggcttgg tgtgcatcgc
     7381 ttcagccggc gtgaggcgcc aacggccgtg cgcggttcgc gtcacgtggc cgtcgtgttg
     7441 gaggcgctcc agcagcgagc tgatgccctc gggccgcagg cccgcctcct tcgcgatcgc
     7501 ggactcgccc atctcgccct cccgcttgag ggctccaaag atcacttgta ggcgttgttc
     7561 gtccacttgt gggcgttgct cgtccatgac gcgcggctac ccactggtgg cgggcggaaa
     7621 ccaagcatgc actacacggc gctgagcacc ggccgactac ccggtggtga gtctcgacac
     7681 ggtgatggcg cgcatcgccg agctccagtc ggccttcaac gcgcccgccg cacaaccctc
     7741 caacaagtca accgccaccg catcgaccgc attcgcgggg gcgctccaat ccgccacggc
     7801 cgcgcaggcc agcgggagcc tcaccgcagt gccggccacg ggtagcggtg tgggcgcgcg
     7861 catggtgcag atcgcgcagg gcgaggtcgg ccagg
//
LOCUS       DASVXS010000124        21861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_582277, whole genome shotgun sequence.
ACCESSION   DASVXS010000124 DASVXS010000000
VERSION     DASVXS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21861
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582277"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..669)
                     /locus_tag="VF752_06785"
     CDS             complement(175..669)
                     /locus_tag="VF752_06785"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX8855290.1"
                     /translation="MNRVASDQLRARAPAPPAHRSRLVQSGIGGVEIGMYLVAAALLL
                     TAATFTVVGTIRDVIEGAGSREIADTGVFILDRVLLLFIIAELLYTLRLVNFGGRILV
                     EPFLYIGLIAVVRRVLVITAEFEQGRSDSTDFLTQIGALSGLAIAFTVAIYLFRRSER
                     QQQT"
     gene            805..1581
                     /locus_tag="VF752_06790"
     CDS             805..1581
                     /locus_tag="VF752_06790"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX8855291.1"
                     /translation="MSYEYEQLVKTIQHKAEASWDEAERAAEATLTTLAERLSGGEAR
                     DIAEELPEEARRWLADGDRAQPFDVDEFLRRVAEREQTDLETAERHARAVFAALTRTL
                     SREELSDMTAELPKNFGRLLPERDRRPPPEPMPTEAFVKHVADRAGIDEEAARRATDA
                     VLETLAEKLAGGEVDDLAEQLADELRQPLRRVRDRKAQPMPLADFVQRVAEREGVSTK
                     EAREHVRAVFQTLREAVTGQEWEDMTAELSNDYTPVLTAA"
     gene            1605..2384
                     /locus_tag="VF752_06795"
     CDS             1605..2384
                     /locus_tag="VF752_06795"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX8855292.1"
                     /translation="MIAYDEFLEIVAQEARLDRDSADRASRAVLETLAERIDAGEARD
                     LAGLLPPELAPSLGHLGPAQAFDLYEFVRRVAEREGVDLDTAELHTRAVFTALGRAVG
                     DKELRDLAAQLPDDFGRLLPAGTHFETITADDFLDRVARRAGMDRGEARRATDAVLET
                     LAERIAGGEVEDLMDRLPVSLHEPLRRGLERTGGTAAGMSLERFVRRVAEREGTSAGQ
                     AAKAARAVLTTLREAVGELSFLDVEVQLPDGYGVVTRLGRS"
     gene            2422..2790
                     /locus_tag="VF752_06800"
     CDS             2422..2790
                     /locus_tag="VF752_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855293.1"
                     /translation="MQLLQADMQRLEDRMRDEKFARELYRALADNKWTKEGESVALSW
                     RRAEELINAVRRGHRAAPLALAETGGEGELDRSIREAFEPLGWRIEPLDTSRHDPDHL
                     SSPEDPPPDEAALKRLRRKS"
     gene            2796..3596
                     /locus_tag="VF752_06805"
     CDS             2796..3596
                     /locus_tag="VF752_06805"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX8855294.1"
                     /translation="MRYDEFVARVSQLAGVAPGEAAKATRAVLQTLSERIGPKESADT
                     ASQLPKELKDAFRPGAVAERFDAAEFVRRVAALTGLDPSVARTRTRAVFATLSEAVSE
                     GELEDWQLGLSTDYVDLAARPAEVGGQPRSGIRGPRRPADSPIGAHDFLLRVAQRAGL
                     EEERARAVTEAVLETFGERIAGGEAEDLAAQLPEPLGAPLVRAGADPEPIPPAEFVRR
                     VAERAGEPPPVAREHARAVLTTLREAVTLKEWRDTEAELPREYDALLA"
     gene            complement(3621..4661)
                     /locus_tag="VF752_06810"
     CDS             complement(3621..4661)
                     /locus_tag="VF752_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8855295.1"
                     /translation="MFRSIHLRLSAAAVTALVVAAAGCGGGGDKSGATVTVSTKPAVQ
                     ASPSAATLQRQFVQVVKTVSPAVVQVQTSSGLGSGVVLDGQGHIVTNAHVVGNFKEFK
                     VTLANGSQHKASLVGTYPPNDIAVVRLDGARPHPASFADSSNIKVGDFAFAIGNPLGL
                     RSSVTEGIVSSVGRTVSEGNGAVIPSAIQTSAPINPGNSGGALVDITGRVMGIPTLAA
                     TDPEFGGAQAPGIGFAIPSNTARTLAAKLITSGHVPQAQRAFLGIKAATIVGGGVLVE
                     GVQKNGPADRAGVPPGDVIVAVAGKPTPTTEALQMVLTGLKPGKSVQVKVLRRTGQRT
                     TLSVKLGTAPGG"
     gene            complement(4819..5016)
                     /locus_tag="VF752_06815"
     CDS             complement(4819..5016)
                     /locus_tag="VF752_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855296.1"
                     /translation="MAVGTSLFLIAAGAILKYAVTAHVEGINLDTAGLVLMIVGIVGL
                     ALSLFLMVRGRSGDLPPPEVP"
     gene            5086..5874
                     /locus_tag="VF752_06820"
     CDS             5086..5874
                     /locus_tag="VF752_06820"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEX8855297.1"
                     /translation="MSGFEFRYRLDGGPPTVRRFRIKGPQTVTRGDIVNLERGEVDLA
                     ATMDTKLLGVSLETKAGSSEPADIEIIVDGDAVYSVDDSEARRRGDYLDVNGLTGAQG
                     VSGGLNQEFMVVVDSPAGDPTLVRIRYGKHGSPAEGADESEARARLNAAVARAVTRVF
                     RSHVGRGPTKAEAFFRRHIVVVVMEDVMTREEHSLAAGGKTETVHEVHNALQGTMKED
                     LVRAVEELTDRKVVAFMSDSQVRPDFASEVFVLDGPVLMSGPRP"
     gene            6319..7131
                     /locus_tag="VF752_06825"
     CDS             6319..7131
                     /locus_tag="VF752_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8855298.1"
                     /translation="MTARSAVAHRMPSGNGRRKPLAAVDERKLVSAAQRGDAAAREAV
                     VEAFEPFIGGAARVYRGSTSVHREELMQEGVVGLLTALRRYDARMGTPFWAYASWWVR
                     QAMQRLVAELGRPVVLSDRALRQLARVKDARSECLRSRGHEPTLVELAGDTGFSKSQV
                     ESLLAVERPPRGLDDPRHGDGGSSDPLVETVADPRAEDEYDRVLPGLETEKLRDLYGS
                     LRGREGAILRARFGLDGPEQTLREIGAGLGLSAERVRQIEERALDKLRAAVC"
     gene            7189..9102
                     /locus_tag="VF752_06830"
     CDS             7189..9102
                     /locus_tag="VF752_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855299.1"
                     /translation="MKSDLRPATAPATDGRVIVTNIPARLDRLPWSRFHWRIVIGLGT
                     VWILDGLQVTVVGSIAPRMTEAGSGVSLSTADVGTAAALYVAGACIGALFFGHLTDRF
                     GRKKLFMLTLGIYMAASVLTAFAFAPWYFFLFRFLTGFGIGGEYSAINTAIDELIPAR
                     NRGRVDIAINGSFWVGAGIGSVASLFFLDQDLFAANVGWRLAFTIGAVLGLGILLVRR
                     NVPESPRWLFIHGYEDEAERIVDQIEAEVRAETGEELPERGESIAVRQRQAIPFREIA
                     RATAKHYPKRGVLGLALVIGQAFMYNAVTFDLGTILSRFFDVGSGAIPVFMVIFAAGN
                     FLGPLLLGRLFDTVGRIPMISGTYLVSAASVAVLAVLFRTDSLTKWSFEALVVATFFF
                     ASAGASSAYLTVSEIFPMETRALAIALFYAIGTAIGGITGPLLFGKFIHSGSADQVAT
                     GFFIGAAAMALGGIAELLFGVRAEGRSLEGIAKPLTALEVEGEPGAVAPAEQTAAERE
                     REERIHDRRARRERYEREGLRRYKPGPGTTFYSPGMLGTAGTTSRYNAMAEEDLDREI
                     ESLQRAFDERGTVSGEELENAVRGEGWGPGRFDDALREAVGEGRVRRVSRDRYGPANG
                     DGRPGREPAPTGR"
     gene            9193..9660
                     /locus_tag="VF752_06835"
     CDS             9193..9660
                     /locus_tag="VF752_06835"
                     /inference="COORDINATES: protein motif:HMM:NF037143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX8855300.1"
                     /translation="MALPVRRSAPPGTRLARRDPFSDLEQLQALTQQLMEGFVPGAGD
                     GEGIAWSPPVDIEETEDAWVVEAELPGVDREDVHVDVEGNELTISGEIKERERTGILR
                     RRTRRTGRFEYRVMLPGDVDADNVDASLHDGVLNVRIPKPERARPHRVEVKAT"
     gene            9837..10634
                     /locus_tag="VF752_06840"
     CDS             9837..10634
                     /locus_tag="VF752_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8855301.1"
                     /translation="MDILVTGGTGTLGRHVVPRLRDSGHDVRVLSRQARGPDEGVEYV
                     TGDLDTGEGVELAVEGADVVVHCAGSTKGDEDKARNLVAAASRAGAPHVVNISVVGAD
                     RVPVVSRVDRVMFGYFASKRGAEHVVAESGLPWTTLRATQFHELMFTTVQQMTKLPVV
                     PAPAGFRFQPVAADEVAARLVELALGEPAGLVPDIAGPRVYEMTELVREYLRSRGKRR
                     PILPLPLPGKAAHAFREGANLAPERAVGRRTWEEFLADRTTSLSQAR"
     gene            complement(10664..10891)
                     /locus_tag="VF752_06845"
     CDS             complement(10664..10891)
                     /locus_tag="VF752_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855302.1"
                     /translation="MSRTKLTGLSEGRGEIERRMSAGEPFGSVEDMINRAELSEDEKS
                     ALWLLAWSMRDTDSQRQETRALLAQLATVGD"
     gene            complement(11068..11796)
                     /locus_tag="VF752_06850"
     CDS             complement(11068..11796)
                     /locus_tag="VF752_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855303.1"
                     /translation="MTGTRVLICDDEPQILRALTLVLSEAGFEVVPAATADEALDKAA
                     LRPPEAAIIDLLLPDGDGVEVCRRLREWSQMPILLLSAVDAEQEKVRALKAGADDFIT
                     KPFATAELIARLEAVLRRATPSADDPIVVVDALEIDLAARTIRAGGREVHLTPIEHDL
                     LRVLVRNRGRLMTHQALLLEVWGPGYETDTHTLRFHISNLRKKIAPAGRREDYVRTQP
                     GVGYRFAPLIPDAGLSRRGAKPRK"
     gene            complement(11813..12199)
                     /locus_tag="VF752_06855"
     CDS             complement(11813..12199)
                     /locus_tag="VF752_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8855304.1"
                     /translation="MRQTVLLVDDEPDILLAARLLLEDAGYAVIEASGGQDALDLMGK
                     TKLDAVFLDLRMPGVDGWDVLEELRGRSTVTRLPVIVLSAHADPSAVERSVALGARGY
                     VRKPFRAADLTRALRTVLQGAPRPTR"
     gene            complement(12171..14453)
                     /locus_tag="VF752_06860"
     CDS             complement(12171..14453)
                     /locus_tag="VF752_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8855305.1"
                     /translation="MPGSAGSAVQQSQGGTKILIVDDQDDVRLALGAVLALDGWETEE
                     AASGEEALARVGRSRHFAGLVVDYRMPGLNGLEVARRIRGTGFERPIIICSAYLSPGI
                     EEEAGAVGARTVSKDDLSRLREAIRQLRPPERHSGNDEAGIGLAAIVESSDDAIIGLT
                     LQGIVATWNAAAERIYGYSAGEMLGRPVWLLVPANHDNDEPEILDKVRRGERVDPYEA
                     VRETKTGHHIDVSLMVSPIKDRQGKIIGASTIARDVTARRRAEERFRGLLESAPDAVV
                     IVGTDGHIALVNRRTEELFGYRREELLGKPVEVLVPERFRNRHPAVRDRYFGDPQVRP
                     MGAGLDLFAVRKDRTEFPVEISLSPFQTEGGTVVSAAIRDVTERKKAEEKFRALLEAA
                     PDAMVIVGTDGLIELVNRRTEELFGYRREELLGKPVEVLVPERLKDRHPLRDDYFADP
                     QVRPMGAGLDLFAVRKDRTEFPVEISLSPLTTETGTIVSAAIRDVTGRKRAEERLRLA
                     LAGERDAVERLRDLDRLKDEFLSTVSHELRTPLTVIGALAEVMRNASAAPRQDQADLV
                     ERIFENAADMSGMIEQLLDYSRLEAGKVALEHRPVHLREAVLRCVELSQAAIGKRDTS
                     LEVPDQLEVQADKRAFERILVNLLTNAAKYSSDGSTIRVIAWTEDGEAVVAVQDDGIG
                     IPSEEQERVFERFYQGSVVSGKRGTGIGLSIVQRYVELLSGRVWVESAPGRGSTFLFT
                     LPLSEGSKERSNASDRVAGR"
     gene            complement(15024..15467)
                     /locus_tag="VF752_06865"
     CDS             complement(15024..15467)
                     /locus_tag="VF752_06865"
                     /inference="COORDINATES: protein motif:HMM:NF008740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX8855306.1"
                     /translation="MRLLLNIIWFVLAGLWLAIGYAIAALICFILIITIPFGIAALRI
                     GVFALWPFGKTVIKRADAGAGSAIGNVIWFVLCGWWLALGHVITGVLLCLTLIGIPLG
                     LANFKLIPVSLLPFGRDIVSIDEARARGLEPHVAVPAHPASNPPR"
     gene            complement(15480..15938)
                     /locus_tag="VF752_06870"
     CDS             complement(15480..15938)
                     /locus_tag="VF752_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855307.1"
                     /translation="MTETELREVLAEYLTLLQGHPSAEAFMAAVLTEDFETGFDGGFT
                     WKGLDGLRDFLSQRAGFFDERHTIKDVLAMDEPADDDVRANTRLEFFLRRWEAPSPTS
                     EEFTGSCYHHWRLRRERDGWRVAAQIVERFDDLNPPAERLFATPDEGLNR"
     gene            complement(16195..16653)
                     /locus_tag="VF752_06875"
     CDS             complement(16195..16653)
                     /locus_tag="VF752_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855308.1"
                     /translation="MAVRWDGDQRGLGIGDALELVSGASELVAAFRQPAWVAEQPETH
                     LLPHVEAWCERDHRLALIDAYIDDTDAYVLDCDWRGAAGNVGAARAAVFSLIGSFAES
                     ATYVRQRRIAGDAGGSTTKLRFEVGTGELAPDTRFAAHGHVVVINVAGVL"
     gene            complement(17064..17318)
                     /locus_tag="VF752_06880"
     CDS             complement(17064..17318)
                     /locus_tag="VF752_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855309.1"
                     /translation="MTDLAYALTTGPPLALTLDGRQITVPLPDELFLTGEMRSAAGGV
                     ELELQLTWSTAKAASYDESGSFAQDRKAVAEPSWPRPGRG"
     gene            complement(17619..20312)
                     /locus_tag="VF752_06885"
     CDS             complement(17619..20312)
                     /locus_tag="VF752_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8855310.1"
                     /translation="MDGSRPALATDSERFPFARGLVPRVPLFRRLSAAQPGGVIVVQG
                     PAGSGKTVLVRSWIEAEALADRVAWVSVERGERDPQRFWLSVVDQLASATDRGELVER
                     VSPSPAFGGEAIVERLLSDLRSLERPFVLVIDDLHELRSEDALRLLERFLGAIPPELR
                     VVLSTREDPQLGLHRLRVADGLAEICGPDLRFSFEETRELMRASGVSLSDSAVALLHE
                     RTEGWAAGLRLAAISLAQHPDPERFVAEFSGSERTVAAYLLAEVLERQPAEVRDLLLR
                     TSVLEWVTGPLADALTGGKGSEATLQDLEEANAFVTSLDAGRTRFRYHHLLTDLLRLE
                     LRRSAPAIIDSLHRAAAQWHEQNGYPVEAIRHAQAARDWVRAARLLADNYVDLVFDGH
                     KATLRALLAAFPAGELDVDAELALAFATARLYDGLLDESAVHIAVADRLAATVPAERR
                     PLFDLRLASATLWLACQRGDIATAKDAMRSFEAVSMDARAGNSDHRASALMNLGIAEL
                     WSLHLDDARRNLEEALALARRIRRPYLEIGCFGHLALTALMSGSAVPTGLRLSEDAVT
                     IAEAHGWGTDRVVAPAVTAGAVAVTWLGRFAEAELWLKRVERAQQSVEEFELEPILHH
                     AHGFVLVGLGRFNEALADFRAAARTQPLLAHTHALPADVRGWIMIAQVLAGDTAAAHA
                     AIAALDAEERDQAGVRIAAASLELAEGSPQDAVDALAPVITHLLEPTAERAPQVLNPR
                     WATVHALLLDAAARDQLGATLAAEASIERALELAEPDGIILPFTLTPVRELLERHPRH
                     RTNHAALLATILDVLGGSPQRSDPETPLSEELSDAELRVVRYLPTNLKAPEIASELFV
                     STNTVRTHLRHIYAKLDAHNRGEAVARARELRLLGPGVQLR"
     gene            20646..21035
                     /locus_tag="VF752_06890"
     CDS             20646..21035
                     /locus_tag="VF752_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX8855311.1"
                     /translation="MQLFATYTFGQALLTVLEFALLFVWIWVAITVVADVFRSKDLSG
                     WAKAGWIFLIVVVPLLGVLVYLVARGDKMRQHELVDQRDQDAALRKYIRSAAATPADE
                     ITELERLRDRGILDEEQFQRAKEKTLA"
     gene            21071..21700
                     /locus_tag="VF752_06895"
     CDS             21071..21700
                     /locus_tag="VF752_06895"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX8855312.1"
                     /translation="METVIAVSFDDPNRLYDAVTKLKELDQQGQVAMYEGRVVERDLN
                     GRLVVKDSVQGGDDDRGVATVSGGLIGLLIGVLAGPVGMLLGGSIGLMTGAVIDMDVD
                     DRDDSVLSAFTRQVQPGQTALFAHLDEQSYEVVDNAMRGMGGNVLRRPALDVESELAA
                     AQEARDVAENEAREKLRKEHREQKKEEIDSKLDALRAKFRKPEKTRAAS"
BASE COUNT         3550 a         7342 c         7556 g         3413 t
ORIGIN      
        1 tgttgcgatc gatcagcgtg acctccaccg gcgctccgcg gagcgccttc accgcttcga
       61 ggccgccgaa gccgccgccc accacgacca cgcggtgttg tggcaaaaga gacgtcacag
      121 ggttggagat agtacgtggc cgccgccccg caaacgacgg tctcgggtgc gccctcaggt
      181 ctgctgttgc cgctcgctcc tgcgaaagag atagatcgcc accgtgaacg cgatggcgag
      241 tccggacagc gctccgatct gcgtcaggaa atcggtggag tcgctacgcc cttgctcgaa
      301 ctcggctgtg atcacgagga cgcgtcgcac caccgcgatg agccctatgt agagaaaggg
      361 ctcgacgagg atgcgtccgc cgaagttcac gaggcgcagc gtgtagagca gctcagcgat
      421 gatgaacagg aggagaacgc ggtcgaggat gaagacgccg gtatcggcga tctcgcgcga
      481 gcctgcccct tcgatcacgt ctctgatcgt gccgacgacg gtgaaggtcg cggctgtcag
      541 cagtagggcc gccgcgacga ggtacatgcc gatctccaca ccgccgatac cgctctggac
      601 gagccgcgag cggtgagcag gcggcgcggg ggcgcgggcg cgcagctgat ccgatgccac
      661 gcggttcaca cgctgctcag cttcacaccc atgtttgggg ttgcaggtag gccgggctcg
      721 cgcgccgtca atggagctct gggtcaacac gatcccagcc cttgttcccc ttcgcggccg
      781 gactatttcc cgccgtggga tcaaatgagc tacgagtacg agcagctggt caagacgatc
      841 cagcacaagg ccgaggcctc gtgggacgag gcagagcggg cggcggaggc cacgctcacc
      901 acgctcgccg aacgactctc gggcggcgag gcgcgcgaca tcgccgagga gctgcccgag
      961 gaggccaggc gctggctcgc agacggtgac cgcgcgcagc ccttcgacgt ggatgagttc
     1021 cttcgaaggg tggcagagcg cgagcagaca gacctcgaga cagccgagcg ccacgcacga
     1081 gccgtgttcg ccgcccttac ccgcacgctg agccgcgaag agctcagcga catgacggcg
     1141 gagcttccga agaacttcgg gcggctgcta ccggagcggg accgccggcc gccgcccgag
     1201 ccgatgccga cagaggcctt cgtcaagcat gtggccgacc gtgccgggat cgacgaggag
     1261 gcggctcggc gtgcgaccga cgcggtgctc gagacgctcg cagagaagct cgccggcggc
     1321 gaggtggacg acctcgcgga gcagcttgcg gacgagctgc gccagcccct gcgacgcgtc
     1381 agagaccgaa aggcgcagcc aatgccgctg gcggacttcg tgcagcgtgt ggccgagcgg
     1441 gagggcgtct ccaccaagga ggcgcgcgag cacgtacggg ccgtgttcca gacgcttcgc
     1501 gaagccgtca ccggccagga gtgggaggac atgacggctg agctctcaaa cgactacaca
     1561 cccgtgctca cggccgcgta ggcgcccgag gtgaccggcc gctcgtgatc gcgtacgacg
     1621 aatttctcga gatcgtcgcg caggaggctc ggctggatcg tgactccgcc gaccgcgcgt
     1681 cgcgagccgt actcgagacg ctcgcggagc gcatcgacgc cggcgaggcg cgggatctcg
     1741 ccggccttct ccctcccgag ctcgccccat cgctcggcca ccttggaccc gcgcaggcct
     1801 tcgatctcta cgagttcgtt cgccgggtcg ccgagcgtga gggtgtcgac ctcgataccg
     1861 cggagttaca cacgcgcgcg gtgttcaccg cgttgggacg cgcggtcgga gacaaggagc
     1921 tgagggacct ggctgctcag ctgccggacg acttcggaag gctgctgccc gccgggacgc
     1981 acttcgagac gatcactgcc gacgacttcc tcgatcgcgt tgcgcgacgg gccggtatgg
     2041 atcgtggcga ggcgcggcgc gcgaccgatg cggtgctcga gacgctggcc gagcggatcg
     2101 cgggcggcga ggtcgaagac ctcatggacc gcctgccggt gtcgctgcac gagccgctca
     2161 ggcgcgggtt ggagcgaacc ggaggaaccg cggccggcat gtcgctcgag aggttcgtga
     2221 ggcgtgtggc ggagcgcgag ggcacgagcg cggggcaggc cgccaaggcc gctcgggcgg
     2281 tcctcacgac gctgcgcgag gcggtcgggg agctcagctt cctcgacgtg gaggtacagc
     2341 ttccagacgg atacggggtg gtgacaagac ttgggcggtc ctgacacaca gacggagcgg
     2401 ttcggcatat cggaggtggc gttgcagctg ctccaagcgg acatgcagcg cctcgaagac
     2461 cgaatgcgcg acgagaagtt cgcgcgcgag ctctaccgcg ctttggcgga caacaagtgg
     2521 accaaggagg gcgagtcggt cgccctcagt tggaggcgag cggaggagct gatcaacgcg
     2581 gtgcggcgag gccaccgcgc ggcgccgctc gcgctcgccg agaccggtgg agagggggag
     2641 ctcgaccgca gcattcgtga ggcgttcgag ccgctcggct ggcggatcga accgctcgac
     2701 acgagcaggc acgacccgga tcacctgagc tcgcccgagg atccgccgcc ggacgaggcc
     2761 gcgctcaaga ggctgaggcg gaagagctag gacagatgcg ctacgacgag ttcgtcgcca
     2821 gggtgagcca gctcgccggg gtggctccgg gggaggccgc aaaggccacc cgagccgtgc
     2881 tccagacgct gagcgagagg atcggcccga aggagtcggc cgacacggcg tcacagctgc
     2941 ccaaggagct caaggatgcg ttccgacccg gggccgtcgc cgagcgcttt gacgcggccg
     3001 agttcgtgcg gcgggtggcc gcgctgaccg ggctcgatcc atccgtcgcg cgcacccgca
     3061 cgcgcgccgt gttcgcgacg ctttcggagg cggtcagcga gggagagctc gaggactggc
     3121 agttggggct ctcgactgac tacgtggacc tggccgcccg ccccgccgag gtcggaggcc
     3181 aaccgcgctc ggggattcgc ggacctcgca ggcctgcgga cagtccgatc ggcgcccatg
     3241 actttctgct gcgcgtcgcc cagcgtgcgg ggctcgagga agagcgggcg cgagctgtca
     3301 cggaggccgt gctcgagacg ttcggcgagc ggatcgccgg cggggaggcg gaggacctcg
     3361 ccgcccagct tcccgagccg ctcggcgcgc cactggtgcg ggccggggcc gaccccgagc
     3421 cgattccgcc cgcggagttc gtcaggcggg tggcagagcg cgcaggcgaa cctccgccgg
     3481 tcgcgcgcga gcacgcacgc gccgtcctca cgacgctgcg cgaggcggtg acgctcaagg
     3541 aatggagaga caccgaggcc gagctgccac gcgagtacga cgcgttgctc gcctaatcag
     3601 cggtcaggtc ggccgccgcc tcagccaccg ggagcggtgc cgagcttgac ggagagggtt
     3661 gtccgctgtc cggtgcgcct gagcaccttc acctgaacgc tcttgcccgg cttgagtccg
     3721 gtcagcacca tctggagcgc ctcggtcgtt ggagtcggct taccggccac ggcaacaatc
     3781 acgtcgcccg gcgggacgcc cgcgcggtcc gccggcccgt tcttctgcac gccctccacc
     3841 agcacgccgc cgcccacgat cgtcgccgcc ttgatgccga ggaacgcgcg ctgcgcctgc
     3901 ggcacgtggc cggatgtgat cagcttcgcg gccagtgtcc gcgccgtgtt gctggggatc
     3961 gcgaagccga tgccgggcgc ctgcgcaccg ccgaactccg ggtccgtggc agccagcgtc
     4021 ggaatgccca tcacgcgtcc ggttatgtcc acgagcgccc ctcccgagtt acccgggttg
     4081 atgggcgcgc tcgtctggat ggcggaggga atcaccgctc cgttcccctc ggacaccgtg
     4141 cgccctacag acgacacgat gccctcggtc acgctcgagc gaaggccaag cggattgcct
     4201 atcgcgaatg cgaagtcgcc caccttgatg ttcgacgagt cggcgaagga ggcgggatgc
     4261 ggtcttgccc catcgaggcg caccacggcg atgtcgtttg gcgggtaggt gcccacgagc
     4321 gaggccttgt gctgggagcc gttggccagc gtgaccttga actccttgaa gttgccgacc
     4381 acgtgggcgt tggtgacgat gtgaccctgc ccgtcgagta ccacgccgga gcccaggccc
     4441 gaggaggtct ggacctgaac gacggccggc gagaccgtct tcaccacctg gacgaattgg
     4501 cgctggagcg tggcggcgga gggtgaagcc tgcaccgccg gcttggtgga cacggtcacc
     4561 gtcgcgccgc tcttgtcgcc gcccccgccg cagccggcgg cagcgacaac gagcgcagtc
     4621 acagcggccg ccgacagacg cagatggatg gagcggaaca tcgaccgacc tctaggtcca
     4681 gataggtcgc ggggccggtt cttcaaaccg gaagcaggcc tgtttgctgg cgcaaatagc
     4741 gggtagcccg accgcgagga cgtgatgacg aaagcgagaa gagctcgcgc tccccggccc
     4801 ggttttctga gcacctgctc agggcacctc cggaggcggt agatcccccg aacgcccgcg
     4861 caccatcaag aacagcgaaa gggcaagccc gacgattccg acgatcatca agacgagtcc
     4921 cgccgtgtcg aggttgatgc cctcgacgtg cgcggtgacg gcgtatttga ggatcgcccc
     4981 cgccgcgatg agaaataggc tcgtgcccac cgccatggtc gctacgcgtg gaatagtccg
     5041 gcgcgtgcgc ccgacaacct cggcggtggg tgcgtaaggg tcccggtgag cggcttcgag
     5101 tttcgctatc gcctcgatgg cggcccgccc acggtgcggc ggttccggat caagggcccg
     5161 cagacagtga cccggggaga catcgtcaac ctggagaggg gcgaggtcga tcttgccgcg
     5221 acgatggaca ccaagctgct cggcgtgtcg ctcgaaacca aggcgggctc gagcgagccg
     5281 gcggacatcg agatcatcgt cgacggcgac gccgtgtaca gcgtcgacga ttccgaggcg
     5341 cgccggcggg gcgactatct cgacgtgaac ggactgaccg gcgcccaggg tgtaagcggc
     5401 ggcctgaacc aggagttcat ggtcgtggtc gactcgccgg cgggcgaccc gacgctagtc
     5461 cggatcaggt acgggaaaca cggctcgccg gccgagggcg cagacgagag cgaggcccga
     5521 gcgcggctca acgccgcggt cgcccgggcc gtgacccgcg tcttcaggag ccacgtcggg
     5581 cgaggtccga ccaaggctga ggccttcttc cgccggcaca tcgtggtcgt cgtgatggag
     5641 gacgtgatga ctcgcgagga gcacagcctc gcggccggcg ggaagacgga gacggtccat
     5701 gaagtgcaca acgcacttca ggggacgatg aaggaggatc tggtgcgtgc ggtcgaggag
     5761 ctcacggacc gcaaggtcgt cgccttcatg agcgacagcc aggttcgccc cgacttcgcc
     5821 tctgaggtgt tcgtgctgga cggacccgtg ctgatgtcag ggcccagacc ctgagtcaga
     5881 gcgggctcgc aaaagttccc gactagcgca aattcgggag ccgttcggag gtttgcaagc
     5941 gctctagcac ggacaaaatc cgcacagtct cggcgctgcg aagcgggctc cgggatctgc
     6001 tccacaagac ggtaaggcgc cgggtgcggg agctcgggac ccatcgtcag gcaagcgaag
     6061 ccggcggagg ttctcgagtg ccctacgttg actgcgcgcg ctgcggcctc acaaccgtca
     6121 gcgcggcgta ccgctgggcg gtcgagggat gcccgagatt gtggagccga actgccccgc
     6181 cccaggcgga cgtcgccggg gcgctcacgg agccggcagt tcggcgacac gcccgcccag
     6241 ggtttcgccg acccaccggg acgcgtcggc ttcggcggcc tacaacccgc ggccccactg
     6301 acccacgtag gagaagccat gaccgctcga tccgccgtcg ctcatcggat gccctcgggc
     6361 aatggaaggc ggaagccgct cgcggctgtg gatgagcgca agctcgtatc ggcggcgcag
     6421 cgtggcgatg cagctgcgcg cgaggcggtc gtcgaggctt ttgagccatt catcggcggc
     6481 gcggctcgcg tctatcgcgg ttcgacctcg gtgcaccgtg aggaactgat gcaggagggc
     6541 gtcgtgggcc tgctcacggc cctccgccgc tatgacgccc ggatgggaac gcccttctgg
     6601 gcatacgcgt cctggtgggt gcgccaggcg atgcagcggc tcgtcgccga gcttgggcgg
     6661 cccgtcgttc tctccgaccg ggcgctgcgc cagttggcgc gcgtcaagga cgcacggagc
     6721 gaatgcctgc gctctcgcgg gcacgagccg acgctcgtgg agcttgctgg cgacacgggc
     6781 ttctccaagt cgcaggtcga gagcctgctg gcggtggagc ggccgccgcg gggtctcgac
     6841 gacccgcgcc acggcgacgg aggatcgtcc gatccactcg tcgagacggt ggcggatcca
     6901 cgagccgaag acgagtacga ccgcgtcctc cccgggctcg agacagagaa gctgcgcgac
     6961 ctgtacggga gcctgcgcgg gcgggaggga gcgatcctcc gcgcgcgctt cggcctcgac
     7021 gggcccgagc agaccctgcg cgagatcggc gccgggctgg ggctcagcgc cgaacgagta
     7081 cggcagatcg aggagcgagc gctcgacaag ctgcgcgcgg ccgtttgttg aaccgcggtc
     7141 tgggtggcct aggccaggag cgagtcttcg tcgcgcgcaa ggagcgccat gaaatcggat
     7201 ttgcgtccag ctaccgctcc ggccaccgat ggtcgcgtga tcgtgacgaa catccccgcg
     7261 cggctggatc ggctgccgtg gtcgcgcttc cactggcgaa tcgtgatcgg cctcgggacg
     7321 gtctggatcc tcgatggcct ccaggtgacg gtggtggggt cgatcgcgcc gaggatgacc
     7381 gaagccggca gcggggtaag cctctcgacc gcggatgtcg gaacggcggc tgcgctctat
     7441 gtcgcgggtg cctgtatcgg cgcgctcttc ttcggccatc tcaccgaccg cttcgggcgc
     7501 aagaagctct tcatgctcac gctcggcatc tacatggctg cgagcgtgct cacagcgttc
     7561 gcgttcgcgc cctggtactt cttcctcttc cgcttcctga ccggcttcgg gatcggaggc
     7621 gagtactcgg cgatcaacac ggcgatcgac gagctcatcc cggcgcggaa ccggggccgg
     7681 gtggacatcg cgatcaacgg cagcttctgg gtcggcgcag gcatcggcag cgtcgcgtcc
     7741 ttgttcttcc tggaccagga cctgttcgcc gcgaacgtcg gctggcggct cgccttcacg
     7801 atcggcgcgg tgctcggcct cggcatcctg ctggtgcgcc gaaacgtgcc cgagagccca
     7861 cgctggctgt tcatccacgg ctacgaagac gaggccgagc ggattgtcga ccagatcgag
     7921 gccgaggtgc gcgcagagac cggcgaggag ctccccgagc gcggtgagag catcgccgtc
     7981 cgccagcgtc aggccattcc cttccgtgag atcgcccgcg ccaccgccaa gcactatccg
     8041 aaacgcggcg tcctcgggtt ggcgctcgtc atcggtcagg ccttcatgta caacgcggtg
     8101 accttcgacc tcggcacgat cctgagcagg ttcttcgacg tcggatccgg cgcgatcccg
     8161 gtgttcatgg tgatcttcgc ggcgggcaat ttcctcgggc cgctcctgct gggacgcctt
     8221 ttcgacaccg tgggccggat cccgatgatc tccggcacgt atctcgtatc cgcggcaagt
     8281 gtcgcggtgc tcgccgtgct gttccggacg gactctctga cgaagtggtc ttttgaggcg
     8341 ctcgtggtcg caaccttctt cttcgcgtcc gccggcgcaa gctctgccta cctgacggtg
     8401 agcgagatct tcccgatgga gacgcgtgcg ctcgccatcg cgctgttcta cgcgattggc
     8461 acggcgatcg ggggcatcac cgggccgctt ttgttcggca agttcattca cagcggtagc
     8521 gccgaccagg tggcaacggg cttcttcatc ggcgccgcgg cgatggcctt gggcggcatc
     8581 gctgagctgc tgttcggcgt gcgcgcggag ggcaggtcgc tcgagggaat tgccaagccg
     8641 ctgaccgccc tggaggtgga aggagagccc ggcgcagttg cgcccgcgga gcagacagca
     8701 gccgagcgcg agcgcgagga gcgcatccac gaccgccgcg ctcggcgcga gcggtacgag
     8761 cgcgaaggtc ttcgtcgcta caagcccggg cccggcacca cgttctactc gccgggcatg
     8821 ctcggcacgg ccggcacgac gagccgctac aacgccatgg ccgaggagga cctcgaccgc
     8881 gagatcgagt cgctccagcg agcgttcgat gagcgcggca ccgttagcgg agaggagctc
     8941 gagaacgctg tccgcggcga ggggtggggt ccggggcgct tcgacgatgc cctccgggag
     9001 gcggtcggcg agggccgggt gcggcgagtg tcgcgcgacc gctacgggcc cgccaacggc
     9061 gacgggcggc cgggccgcga acccgcgccc acgggacgct gatcgaggcg tcgctcgctc
     9121 gttcgagcgg gccttgactg cgcgcgcacg ccgcctatct cgacagttga cgccccagtg
     9181 aaggaggaca ccatggcctt gccagttcgt cggtcagcac caccgggcac caggctcgcc
     9241 cgccgggacc cgttcagcga tctcgagcag ctgcaggcgc taactcagca gctcatggag
     9301 ggattcgtgc ctggagcagg agacggtgag ggcatcgcat ggtcgccgcc cgtcgacatc
     9361 gaggagaccg aggacgcatg ggtcgtcgaa gcggagctcc ctggcgttga tcgcgaggac
     9421 gtacacgtag acgtcgaggg caatgagctc acgatctccg gcgagatcaa ggagcgcgag
     9481 cggacgggga tcctgcgccg gcgtacgcgc cggacgggac ggttcgagta tcgagtcatg
     9541 cttccgggcg atgtcgacgc cgacaacgtg gacgcatcgc ttcacgacgg agtgttgaac
     9601 gtccgcatac ccaagcccga acgtgcccgg cctcaccgag tggaggtgaa ggccacgtag
     9661 cagccgcatc cgcgggcgca tcgtgagaag gccgcgaccg gttctgctcc ccgcgtctcg
     9721 cgcgctgcgc ccagctcgac ggcgcgctcg tcggcccgta gcacgacctc cgggtccatc
     9781 accgcgagga gcgcctcgat gtccccgttg cgcgccgccg cgagaaacga atcaacatgg
     9841 acattctcgt aacaggcggc acggggacac tcgggcgcca cgtggtcccg cggctccgtg
     9901 acagcggcca cgacgtacgg gtgctcagca ggcaagcccg cggccccgat gaaggcgtcg
     9961 agtacgtgac cggtgacctc gacaccggcg agggagtcga actcgccgtg gaaggtgccg
    10021 atgtcgtcgt gcactgcgcg ggcagcacca aaggcgacga ggacaaggcg cggaacctgg
    10081 tcgccgcggc gtcgcgggcg ggggcgcccc acgtggtgaa catctccgtc gtcggcgcgg
    10141 atcgcgtgcc ggtcgtcagc cgcgtcgacc gcgtgatgtt cggctacttc gcatcgaagc
    10201 ggggcgcgga acacgtggtc gccgaatccg ggctgccgtg gaccacgcta cgcgccactc
    10261 agttccacga actgatgttc acgacggtgc agcagatgac caagttgccg gtcgtacctg
    10321 ccccggcagg gttccgcttt cagcccgtcg ccgctgacga ggtggcagcc cggctggtgg
    10381 agcttgcgct aggcgagccg gcgggcctgg tgcctgacat agccgggccg cgggtgtacg
    10441 agatgacgga gctggttcgc gaatacctgc ggagccgggg caagcgccgc ccgatcctgc
    10501 cgcttccgct cccgggcaag gccgcgcacg cgttccgcga gggagccaac cttgcccccg
    10561 agcgggcggt cggccgtcgt acctgggagg agtttctcgc cgaccggacg acgtcgctca
    10621 gtcaggccag atgagtaccg cgcggtgctt gacggctgac ggttcaatcg ccaaccgtgg
    10681 ccagttgtgc cagcagtgcg cgggtttcct gtcgctgcga gtccgtgtcc cgcattgacc
    10741 aggcaaggag ccagagggcc gacttctcgt cctcggagag ctctgctcga ttgatcatgt
    10801 cctccacgct tccgaaaggc tcgcctgcgc tcatcctgcg ctcgatttcg cctcggcctt
    10861 cggaaaggcc cgtcagtttg gtgcgggaca tgcccactcc tctcgcccgt gcccaagttg
    10921 atctgaggag ggctcggttt cgggtgtctg tgcgaggcgc ttggccacgt cggggggcgc
    10981 tcgactattg caactggggc gtgaggctcg cgtgaggatc tgtgcggtgc gggcacagga
    11041 tcttgttaga gattggcgat cttccagcta tttgcgcggc ttcgcccctc ggcgagagag
    11101 gccggcgtcc gggatcagcg gtgcgaagcg gtatccgacg cccggctggg tgcggacata
    11161 gtcctctcgc cggcctgccg gcgcgatctt cttgcgaagg ttcgagatgt ggaatcgcag
    11221 cgtgtgggtg tcggtttcgt agccgggccc ccagacctcg agcagcagag cctggtgtgt
    11281 catcagcctg ccgcggtttc gcaccagcac acgcagcagg tcgtgctcga tcggcgtcag
    11341 gtggacctcg cgcccaccgg ctcggatggt tcgagcggcc aggtcgattt ccagggcgtc
    11401 gacgaccacg atcgggtcat cggcgctcgg agtcgcgcgg cggaggacag cctccagccg
    11461 tgcgatcagc tcggccgttg cgaacggctt ggtgatgaag tcatccgcgc ccgccttcaa
    11521 cgcgcgcacc ttctcttgct cggcgtcgac cgcagaaagc aggaggatcg gcatctggct
    11581 ccattcacgg aggcgccggc acacttcgac cccgtctccg tccggcagca gcaggtcgat
    11641 gatggccgcc tccggcgggc ggagcgcggc cttgtcgagg gcctcgtcgg cagtcgccgc
    11701 cggcaccacc tcgaagccgg cttccgacag gacgagggtg agagcccgga ggatttgcgg
    11761 ctcgtcgtcg cagatgagga cacgcgtgcc cgtcaccgtg cggacatgcc tttcatcgcg
    11821 tcgggcgagg tgcgccctgc aagaccgtac gaagcgctcg cgttagatcg gcggctcgaa
    11881 agggctttct gacgtagccg cgcgccccga gcgccacgct tcgctcgacc gcggacgggt
    11941 cggcatgagc ggagagcacg atcacgggca ggcgcgtgac cgtgcttcgg ccacgaagct
    12001 cctccagcac gtcccacccg tccacgcccg gcatccgcag gtccaggaag acagcgtcaa
    12061 gttttgtctt ccccatcagg tcgagcgcgt cctggccccc gcttgcctcg atcaccgcgt
    12121 aacccgcatc ctccagcaac aggcgcgcag cgagcagaat gtcgggctcg tcatcgacca
    12181 gcaacacggt ctgacgcatt actccgctcc ttcgacccct ccgacagcgg caacgtgaag
    12241 aggaacgtcg atccccggcc aggcgcgctc tcgacccaga ctctgcctga gagcagctcg
    12301 acgtaccgct gcacgatgct gagccctatg cccgtgcccc gcttccccga gaccacggag
    12361 ccctggtaga agcgctcgaa aactcgctcc tgctcctcgg acggaattcc gatgccgtcg
    12421 tcctggacag caacgacggc ttcgccgtcc tcggtccagg cgatcacgcg gatcgtcgag
    12481 ccgtccgatg agtacttggc tgcgttcgtc agcaggttca cgaggatgcg ctcgaacgcc
    12541 cgcttgtcgg cctggacctc cagctggtct ggaacctcga gcgaggtgtc gcgtttgccg
    12601 atcgcggcct ggctcagctc gacgcagcgc agcacggctt ctcgcaggtg aaccgggcgg
    12661 tgctcaaggg ccaccttgcc ggcttccaag cgcgagtagt cgagaagctg ctcgatcatc
    12721 ccgctcatgt ccgcggcgtt ctcgaagatg cgctccacaa ggtcggcctg atcctgacgt
    12781 ggtgccgcgc tcgcgttgcg catgacttcc gccaaggcgc cgatcaccgt tagcggcgtg
    12841 cggagctcat gtgaaaccgt cgataggaac tcgtccttca gccggtcgag gtcacgcagg
    12901 cgctccaccg cgtccctctc tcccgccagc gctagccgca gcctttcctc tgctctcttc
    12961 cgaccagtca catcccggat cgccgctgag acgatcgtcc cggtttcggt tgtgagggga
    13021 ctgaggctga tctccaccgg aaactcggtt cggtccttgc gcacggcgaa gaggtcgagg
    13081 ccggcgccca tcgggcgcac ctgtggatcg gcgaagtaat cgtctctgag ggggtggcga
    13141 tctttcaagc gctcgggcac gagcacctcc accggcttcc ccagcagctc ctcgcgccgg
    13201 tagccgaaca gctcctccgt ccggcgattg acgagctcga tcagtccatc ggtgccgacg
    13261 atcaccatcg cgtccggtgc cgcctccaag agggcgcgga acttctcttc tgctttcttg
    13321 cgctccgtca cgtctctgat cgccgcggag acgaccgtcc cgccctcggt ctggaacgga
    13381 ctgaggctga tctcgaccgg aaactcggtt cggtccttgc gcacggcgaa gaggtcgagc
    13441 ccggcgccca tcgggcgcac ctgtggatca ccgaagtaac ggtctctgac ggcggggtgg
    13501 cgatttctga agcgctcggg cacgagtacc tccaccggct tccccagcag ctcctcgcgc
    13561 cggtagccga acagctcctc cgtccggcga ttgaccagcg cgatgtgtcc gtcggtgccc
    13621 acaatcacca ccgcgtctgg tgccgactcc aagagcccac ggaacctctc ttccgctcgc
    13681 ctgcgcgccg tgacgtcgcg ggcgatcgtt gacgctccga tgatcttccc ctgacgatcc
    13741 ttgatcggcg agaccatcag cgagacgtcg atgtgatgcc cggtcttggt ttcccgcacg
    13801 gcctcatagg ggtcgacgcg ctcgccgcgc ctgaccttgt cgagaatctc gggctcgtcg
    13861 ttgtcgtggt tcgcgggcac gaggagccag accggcctgc cgagcatctc cccggcggag
    13921 tatccgtaga tcctctccgc cgctgcgttc caagtcgcga cgatgccctg caacgtcaga
    13981 ccgatgatcg cgtcgtcgga ggattcgacg atcgcggcca ggccgatgcc agcttcgtcg
    14041 ttcccgctgt ggcgctcagg gggccgaagc tgccggatcg cctcgcggag ccggctgagg
    14101 tcgtccttgc tgactgtgcg cgccccaacc gcgcctgcct cttcttcgat ccccgggctc
    14161 aggtaggccg agcagatgat gatcggacgc tcgaatccgg ttcctcggat tcgtcgtgcg
    14221 acctcgagcc cgttgaggcc cggcatcctg tagtcgacca ccaggccggc aaaatgacgg
    14281 ctccgcccca cccgcgccag ggcctcttca ccggacgccg cctcctccgt ctcccagcca
    14341 tcgagtgcga gcacggcccc aagcgccagc cgtacgtcat cctggtcgtc gacgatcagg
    14401 atcttcgtgc ccccctggga ctgctgcacc gccgatcccg cgctcccggg catcaggcgg
    14461 cgcgcccttg ctggcggctc actcggcgca agctcggcga acgcaagcta gtgcgagtgc
    14521 atcccattgg tgccgttctc ctcgctgtct ccagttagcg cgtcgcggta gctgtccagc
    14581 gccccgcgga gctcgcgttc gtaagcgtcg tcagcggttt cgatcgcacg gagtagaccg
    14641 acggcctttg cggcccaatc tcgggtcacc ccgtcgtgca ccgccatcgc ctgctggaga
    14701 gcgtcggcgg tgtcggcatc ttccagggcc tgctggaagc gctcgtctcc ccgtatccgc
    14761 cccggcagtg cagcctcgaa ggcagcggcg gccggcgcga gcgcgcgttg gcgccggtac
    14821 agggcggccc agagtcgcag ggccagcctt atcttcggat tgcgagtccg gtcgggcgtg
    14881 ggcgaggcct ccccggggat gtctgtcgtg ggtcgggcct ggcccgaata ctcacatgcc
    14941 gagctgactg cggcgtcagt ccgtggggtt ttcgtcccga atttgcgagg ttttctcagc
    15001 cacaaccaga cgtggctgcc gactcagcgc ggcgggttcg aggccggatg cgcgggcacg
    15061 gcgacgtgtg gctcgagccc gcgcgcccgc gcctcgtcga tcgagacgat gtcccggccg
    15121 aacggcagca gcgacaccgg gatcagcttg aagttggcaa gcccgagcgg gatcccgatc
    15181 agtgtcaggc agagcagcac gccggtgatc acgtggccga gcgcgagcca ccagccgcat
    15241 aggacgaacc agatcacgtt ccctatcgcc gagccggcgc ccgcgtcggc gcgcttgatg
    15301 acggtcttgc cgaagggcca cagagcgaag acgccgatgc gcagcgctgc gatcccgaac
    15361 gggatggtga tgatcaggat gaagcagatc agcgccgcga ttgcgtaccc gattgcgagc
    15421 cacagcccgg cgagcacgaa ccagatgatg ttcaacagca gacgcatggc tacctccgtt
    15481 caacggttca ggccctcgtc cggggtcgcg aacagccgct cggcgggcgg attgaggtcg
    15541 tcgaagcgtt cgacgatctg cgcggccacc cgccagccgt cccgctcccg gcgcaggcgc
    15601 cagtggtggt aacaggagcc ggtgaattcc tcgctggtgg gcgacggtgc ctcccagcgg
    15661 cgcaggaaga actcgagccg cgtattggcc cggacgtcat cgtccgcggg ctcgtccatg
    15721 gcaaggacgt ccttgatcgt gtgccgttcg tcgaagaacc cggcacgctg ggagaggaag
    15781 tcgcgcagcc cgtccaggcc cttccacgtg aagccaccgt cgaagccggt ctcgaagtcc
    15841 tcggtaagca ccgcggccat gaaggcctcg gcgctcgggt ggccctgtag gagcgtgagg
    15901 tactccgcca gcacctcgcg cagttcggtc tccgtcatca ggcgggcggg tcgctcgggt
    15961 cccgaagcga gtatcgcaag gacgcaggaa cgccgctagg cgtggcggta cgagagtagg
    16021 cagatctacc acattgaccg ccagccgccg acgatcgtca acgcgggcga ggctttgatg
    16081 gttccggccg agacggtgga cgcggtgagg aacgtcggca gcgggaacgc ggcggagctc
    16141 gccacgtaca tcgtcgagaa ggggaagccg tttctcgtgg tcgtcgggtg acggctagag
    16201 cacacccgcc acgttgatca caaccacgtg gccgtgagca gcgaacctcg tgtcgggagc
    16261 aagctcgccg gtgcccacct caaaccgcag tttcgtcgtc gagccgccgg catcgcctgc
    16321 gatacggcgc tggcgaacgt aggtcgcact ctcggcgaaa gacccgatca gagaaaacac
    16381 ggccgccctc gccgccccga cattgccagc cgcaccgcgc cagtcgcaat cgaggacata
    16441 cgcgtccgtg tcgtcgatgt aggcgtcgat caaggcaagt ctgtggtctc gctcacacca
    16501 cgcctcgaca tgtgggagga gatgcgtctc cggctgctcg gccacccagg ccggttgcct
    16561 gaatgctgcg acaagctcgc tggcgccgga aacgagctca agggcatcac cgatgccgag
    16621 cccacgctgg tcgccatccc accggaccgc cacaggcgca cgctacacca ggggcgagga
    16681 tcgcctggcg aaagactgcg gcggcgacgc cgctcatctc cgtgccggtg tgattcgatc
    16741 gttggccgtt ccccgcgcga taggcgcagc gtctccgctc cgttggacga agagccactg
    16801 cctgggtaga cggtcacgtc cggtccttct cgcgcgcgca atggatcagg cgcgtgacgt
    16861 cggtgacgag agccgcatat gtggtggcca caccttcctc ccgatcgaac gccgcgatgt
    16921 acgccccata ccgctcagcg cgctcagtcg gcgcggcggc ccactggctg taggtctgcg
    16981 cgaccgcgat cgcgaggatg agtcgtcgtc gcgcgaactg cgtgattcag aggcggagcc
    17041 gggtgtcccg accagggtct acgtcatcca cgaccaggtc gaggccatga cggctcggcg
    17101 accgccttgc ggtcctgagc aaacgagccg gattcgtcgt agcttgcggc cttcgcggtt
    17161 gaccaggtca gctggagctc gagctcgacg ccgccagccg cggacctcat ctcacccgtc
    17221 aggaacagct cgtcggggag cggaacggtg atctgccgcc cgtccagcgt cagcgcgagc
    17281 ggcggacccg tggtgagtgc gtaagcgaga tccgtcaaac gctcagctgc ctgctggcga
    17341 gacacacttt cgctctccgt gcggttgaag cggctcatcg gctcgccgga ttaaccctcg
    17401 tgcctgggtg agccgtcgtc acctacttcg gacgattgcc gcgtcacggc tcgttttgcg
    17461 cacgatagcg gcgcgttcgc ggcgcgcgat ttcattcctg agtggtggct gtggatgatc
    17521 ctcggcttgc tcgagattcc gctcggcgtg ctcgcgcttg cggccccggg cgccacgctg
    17581 gccgccactg ctcgcccgct cttcggcggt ccgcttccct agcgcaactg tacgcccggt
    17641 ccgagcagcc gtagctcccg tgcgcgggcg accgcctcac cgcggttgtg ggcatcgagt
    17701 ttcgcgtaga tgtggcgcag gtgggtgcgc acggtgtttg tcgagacgaa cagctcggag
    17761 gcgatttccg gggccttgag gttggtgggc aggtacctca ccacgcgcag ctcggcgtcg
    17821 ctgagttcct cgctcagtgg tgtctccggg tcggatcgct gcggcgaccc accgagcacg
    17881 tcgaggatcg tggcgagcag ggccgcgtgg ttggtccggt gccgcgggtg gcgctcgagc
    17941 aactctcgaa cgggagtaag cgtgaacggc aggatgatcc cgtccggctc ggcgagctcg
    18001 agcgcgcgtt cgatcgatgc ttcggcggcg agcgtggcgc cgagttgatc gcgcgcggca
    18061 gcgtcgagca gcagcgcgtg gacggtcgcc cagcgcgggt tcagcacctg cggagcgcgc
    18121 tcggcggtcg gctcgagcag gtgcgtgatc accggcgcga gcgcgtccac ggcgtcctgc
    18181 gggctgccct cggccagctc gagcgaggcg gcggcgatgc gcaccccggc ctgatcgcgc
    18241 tcctcggcgt cgagcgcggc gatcgcggcg tgcgccgccg ccgtgtcgcc ggccagaacc
    18301 tgcgcgatca tgatccaacc gcgcacgtcc gcggggagcg cgtgcgtgtg cgcaagcaac
    18361 ggctgagtcc tcgcagcagc tcggaaatcc gccagggcct cgttgaatcg accgaggccg
    18421 accagcacga agccgtgcgc gtggtggagg atcggctcta gctcgaactc ttctacggat
    18481 tgctgcgcgc gctccacccg cttcagccac agctcggcct cggcgaagcg accgagccac
    18541 gtgactgcga ccgcgcccgc cgtgaccgcc ggagcgacga cgcgatccgt tccccacccg
    18601 tgtgcctcgg cgatcgtcac cgcgtcctcg ctcagccgca gtccggttgg gaccgccgag
    18661 ccgctcatga gcgccgtaag cgcgaggtgg ccaaagcagc cgatctcgag atacggccgg
    18721 cggatgcggc gggccagtgc cagcgcctcc tcgaggttgc gccgggcatc atccaggtgc
    18781 aacgaccaga gctcggctat tccgaggttc atcagcgccg aggcgcgatg gtcgctgttg
    18841 cccgcccgcg cgtccatcga gaccgcttcg aaagagcgca tcgcgtcctt cgccgtcgcg
    18901 atatcaccgc gttgacacgc cagccagagc gttgcgctcg cgagacgcag gtcgaagagc
    18961 ggcctgcgct cggcgggaac cgttgcggcg agccggtccg cgaccgcgat gtggaccgcg
    19021 ctctcgtcca gaaggccgtc gtagagccga gcggtggcga acgccagggc cagctctgca
    19081 tccacgtcga gctcgccggc cgggaaggcg gccagcagcg cccgcagcgt cgccttgtgc
    19141 ccgtcgaaca ccagatccac gtagttgtcg gcgagcaggc gggcagcgcg tacccagtcg
    19201 cgtgccgctt gggcgtgtcg gatcgcttcg accgggtagc cgttttgctc gtgccactgc
    19261 gcggccgcgc ggtggagcga gtcgatgatc gccggcgcag accggcgcag ctcgagccga
    19321 agcaggtccg tcagcaggtg gtggtagcga aaccgcgtcc gtcccgcgtc gagcgaggtg
    19381 acaaacgcgt tcgcctcctc gaggtcctgc aaggtcgcct ccgacccctt gccgcccgtt
    19441 agggcgtcgg caagcgggcc ggtcacccat tccaacacgg aggtacgcag aagcaggtcg
    19501 cgcacctccg ccggctggcg ctccagcacc tccgcgagca gataggccgc caccgtccgc
    19561 tcgcttcccg agaactcggc cacgaagcgc tcggggtcgg gatgctgggc gagcgagatc
    19621 gccgcgagcc gcagccccgc agcccagccc tcggtccgct cgtggagcag cgccaccgcg
    19681 ctgtcggaga gcgacacccc gcttgccctc atcagttcgc gtgtctcctc gaacgagaag
    19741 cgcagatccg ggccgcagat ctcggccagt ccgtccgcca cgcgcaggcg atgcaggccg
    19801 agctgtggat cctcgcgcgt cgacagcacc acccgcagct cggggggaat cgcgcctagg
    19861 aagcgctcga gcaaccgcag cgcgtcctcc gagcgaagct cgtgcaggtc gtcgatcacc
    19921 agcacgaacg gccgctccag cgagcgcaga tcggacaaca gtcgctcgac gatcgcttcg
    19981 cccccgaagg cgggcgacgg acttacccgc tcgaccaact cgcccctgtc ggtcgccgac
    20041 gccagctgat caaccaccga cagccagaat cgctgcgggt cccgctcgcc gcgctcgacc
    20101 gacacccacg ccactcgatc tgcgagtgcc tccgcctcga tccacgaccg gaccaggacg
    20161 gtctttccgc tgcctgctgg gccctggacc actatcacgc cgccgggttg ggccgcggac
    20221 aggcgccgaa acaacgggac acgcggcacg agcccgcgtg cgaaagggaa acgctccgag
    20281 tcggtggcga gcgctggtcg cgatccatcc atcggggcct cgagatctca agggccggcg
    20341 tctggtctcg ctgagcatct caaaactcac gtagccgagc aagacggagc acgcggcggg
    20401 ctcgccatgg ccgcttgccg agatcgtcaa gaatgcgtga cgactgctca cccagccagc
    20461 cctcaggctc ctacacaacc cacgaccgca aggagaccga gctggcgctg tcggacgaag
    20521 tgagcaacct cgcggcgagg gcgaaggaaa tgcaggcccg gaccgcagca tcgtgatcga
    20581 cagccggcga taggcgccag cacctgagtc ggggctcgca atcacgcaaa ccggaaggag
    20641 gctgcatgca gctcttcgca acgtacacct ttggtcaggc ccttctcacc gtcttggagt
    20701 tcgcgcttct gttcgtgtgg atctgggtcg ccatcaccgt cgtggccgac gtattccgca
    20761 gcaaggatct ctcgggctgg gcgaaggccg gctggatctt cctgatcgtg gtcgtgccgc
    20821 tgctcggggt gctggtctac ctggtcgccc gcggcgacaa gatgaggcag cacgagctcg
    20881 tcgaccagcg cgaccaggac gcggctcttc ggaagtacat ccgcagcgct gccgcgaccc
    20941 cggccgacga gatcaccgag ctcgagcgcc tcagggaccg cgggatcctc gacgaggagc
    21001 agttccagcg cgccaaggag aagacgctgg cctagcgcca cgaggacatc acacgaaagg
    21061 agacaacacg atggaaaccg taatcgccgt ttcgttcgac gatccgaacc gcctgtacga
    21121 cgccgtgacg aagctcaagg agctcgatca gcaaggccag gtcgcgatgt acgagggccg
    21181 agtcgtcgag cgagacttga acgggcggct ggtcgtcaag gacagcgttc aagggggcga
    21241 cgacgaccgc ggagtcgcga cggtgtcggg cgggcttatc ggtctgctga taggcgttct
    21301 cgccggacct gtcggaatgc tgctcggcgg gtcgatcgga ttgatgaccg gcgctgtaat
    21361 cgacatggac gtcgacgacc gcgacgacag cgtcctgagc gcgttcaccc gtcaggtcca
    21421 gcccggacag accgccttgt tcgcgcacct cgacgagcag agctacgagg tcgtggacaa
    21481 cgcgatgcgc ggcatgggtg ggaacgtcct ccgacgcccc gccctggacg tggaaagcga
    21541 gctcgcggct gcccaggaag cgcgcgacgt cgccgagaac gaggcccgcg agaagctccg
    21601 aaaggagcac cgggagcaga agaaggagga gatcgacagc aagctcgacg cgctgagagc
    21661 gaagttccgc aagcccgaga agacgcgtgc ggcgtcctga cggcgccggc gctgactgag
    21721 aggagactcc gtgtcccacg acccgcaact cgaggaagac gtccgaacgg ctctggccaa
    21781 ggaccccggc ttggagaacg agcccatcgc ggtatccgga cgcgccggaa acgtcacgtt
    21841 gcgcggcacg gtctcgagcc a
//
LOCUS       DASVXS010000125         7294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_795146, whole genome shotgun sequence.
ACCESSION   DASVXS010000125 DASVXS010000000
VERSION     DASVXS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795146"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF752_06900"
     CDS             <1..281
                     /locus_tag="VF752_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855313.1"
                     /translation="FTYDVAGTRAGELDTDLALEPTRHYDVYTSVTSITVDGAGLPSA
                     SDLTELDPVGQMHVMQQSIFGALKRALFDVIQFFNGLMRRLRAKRGER"
     gene            278..1453
                     /locus_tag="VF752_06905"
     CDS             278..1453
                     /locus_tag="VF752_06905"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8855314.1"
                     /translation="MISDFHSHYPLHLVPEARGDLLDLITTPKGRHRLRDRIRALLVG
                     LASRFGNYRSFESGPRVTVPQLQAGGFGVAWSVLYSFFDEVDLSQSYGAPPQPGYVED
                     LLSELDLVEEEIATHHAGAATVARNPAELDAALVAGQVALVHCVEGGYHLGATPQAID
                     AAVTQIARRGVAYVILAHLFWRRVATNANAVPFIPDPIYRFVFPQPSVGLSELGEAAV
                     RAMVRERVLIDVSHMSERARARTLELLDEIDPAKSTPLLASHAGYRFGKQEYNLTDDE
                     VRAIAARDGVIGLIFAQHQLLDGLRKRTTTFDESFEAICAHIDKIRDLTGSHRHVGLG
                     SDFDGFIKPTLGGLESAADMAQLEAALVSHYGAGDGAAICSGNALRLLRSYWRGKPA"
     gene            complement(1450..1884)
                     /locus_tag="VF752_06910"
     CDS             complement(1450..1884)
                     /locus_tag="VF752_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8855315.1"
                     /translation="MIGSIVVGIDGSDTAARAAQRAAELANALGAKVHLVSAYKPMSG
                     ARVGGEPGPEAAQWTVQRSMHADSVLEQAAVRMRNAGLDVHCHAVNGDPAEALLNVAD
                     EENADLILVGNRGMTGARRMLGSVPNKLSHHANCDVMIVATS"
     gene            complement(1938..2219)
                     /locus_tag="VF752_06915"
     CDS             complement(1938..2219)
                     /locus_tag="VF752_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8855316.1"
                     /translation="MSDDKPYWMLLYEYVPDIVERRAPYRDAHLALIRRWKESGRLEL
                     AGALGDPPYGGALVFRVDDPGEVDEFTAEDPYVANGLVTTRRVEPWKVV"
     gene            complement(2235..3509)
                     /locus_tag="VF752_06920"
     CDS             complement(2235..3509)
                     /locus_tag="VF752_06920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855317.1"
                     /translation="MVRSLLRSLTPDLAPFRESRDYRLLSAGALVTGLGTQAALVALP
                     YQVYVLTHDPLLVGLLGVVELGPLVAASLIGGALADRHDRRTMLLLCQLALVAVAGSL
                     AAAAFAGNPPVWLLYVLAGAMAGAGQLERVARQSMVPNLASPENLRAALSTNFGLMQL
                     TMVVGPALGGLLISWLGVGAAYTVDAVSCLGMALAAAAMSPRPPIGVEEHEPIGRSIA
                     TGLRFVRGDRAVMGSFAIDLLAMTFGMPRALFPVLALSVYHAGATGTGGLYAAVSAGA
                     TVAALTTGWLGQARYLGRIVFGAVGVWGLAVGAAGLMTTIWPALVLFAIAGAADSVSA
                     VCRSTIAQTLTPDRMRGRMSAVFSLVVAGGPRLGDVESGTVASVASPRFSVVSGGLAC
                     VAGLGLIAVLFPELYAYDGDRVTADVAAAAAA"
     gene            complement(3520..4554)
                     /locus_tag="VF752_06925"
     CDS             complement(3520..4554)
                     /locus_tag="VF752_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8855318.1"
                     /translation="MEHRMLGRTGVNVSPLCLGAMMFGRWGNRDHDESIRIIHAALDA
                     GINFVDTADVYSGGESEEIVGKALAGGRRDHVVLATKFHGPMGEDPNERGNSRRWIMQ
                     EVDNSLRRLQADWIDLYQVHRPEPSTDIDETLGALTDLVHAGKIRYLGHSTFPAHAIV
                     EAQCVAERRGRERFASEQPPYSLLVRGIEKDVLPVCQQYGLGVIPWSPLAGGWLSGRW
                     RKGVEAPTSTRAQRLPARYDLSKPENQRKLDAADALLRLAEQQGLSLVHLAIAFVLQH
                     PAVSAAIIGPRTIEQLESQLGAADVRLPPEVLDKVDEIVPPGTNVNPADAGYEPPAIA
                     DPSLRRRDPR"
     gene            4658..5128
                     /locus_tag="VF752_06930"
     CDS             4658..5128
                     /locus_tag="VF752_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855319.1"
                     /translation="MSGLARLRRGLVPALVALLALLCATSAVAATRKSHKVNATITAA
                     PLTAANGVTTVTGIVTGKPFPGEGAVVYRVKTGATGTITANVTVFTRNGIVKGSTVAT
                     VTPGANNTSTLTGTAKVASGTGNFAGATGKDLKVTGTIDADGVLHVNVTGSIKY"
     gene            5219..6628
                     /locus_tag="VF752_06935"
     CDS             5219..6628
                     /locus_tag="VF752_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8855320.1"
                     /translation="MSDARRPSLDVARILEERAGEQLALNDRHLNPQFGRVLRTIGYD
                     RNWKHASGAYLFDENGDRWLDMLGGYGVFALGRNNAAVRRQLERFLATETATLPQLGV
                     TLLAGVLAEQLLARSPSRLGAVLFTNSGTEAVEGAMKLARAATGRPRIVYCDHAFHGL
                     TLGSLSVNGNEEFRERFGPLLPGCDPVPFGDLDALRSELARGDVAAFLVEPVQGKGVV
                     IPPDGYLQEAQELCRRQGTLCIADEVQTGLGRTGRFLALEHWGLEPDMVLVSKALSGG
                     YVPVGAVLAARSVFERVFDTMERAVVHGSTFGTNDFAAAAGIATLAELDDADLVERSR
                     RSGERLLELTRPLVERYEVVRDVRGIGLMWAIEFGPPAGGAALRAWKVAESRQPGIFA
                     QLVTVPLFNEHRILTQVAGHRMNVVKALPPLVIEDDDLVHFADSLDQVLAKLERGPAR
                     AFARFGLDAARRSLRPSRA"
     gene            6683..>7294
                     /locus_tag="VF752_06940"
     CDS             6683..>7294
                     /locus_tag="VF752_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855321.1"
                     /translation="MRRRLREHPYALTALALAFAATAVAGIANAYGFGAVDSAFDAIE
                     PGWIAFIAVAEIVSYGAYVVAYRSIACVQGHEPIPLRLAARVVAAGFGPLSAGGGFAL
                     DKQALHALHSDERAATVRVVGLGVLEWALLAPAASICAIAMLLGNRDGVWPSLLWPWA
                     LAVPAGFALGLFASAPGTRRRILRWFGNPGWLGHLLEGVATLHT"
BASE COUNT         1053 a         2641 c         2536 g         1064 t
ORIGIN      
        1 cgtttaccta cgacgtcgcg ggtacgcggg caggcgagct cgacactgat ctcgccctcg
       61 agcccacgcg ccactacgac gtctacacaa gcgtcacgtc gatcacggtc gacggcgccg
      121 ggctcccctc cgcgtccgac ctcacagagc tcgaccccgt gggccagatg cacgtgatgc
      181 agcagagcat cttcggcgcg ctcaagcgtg cgcttttcga cgtgatccag ttcttcaacg
      241 ggctgatgcg ccgcctgcgc gcgaagcgcg gcgaacgttg atatccgact tccactcgca
      301 ttacccgctg cacctcgtgc ccgaggcgcg cggcgacctg ctcgacctga tcacgacgcc
      361 caaaggacgc caccgcctgc gcgaccgcat ccgcgcactc ctcgtgggcc tggccagccg
      421 cttcggcaac taccgctcgt tcgagtccgg cccgcgcgtc accgtgccgc agctccaggc
      481 cggcggcttc ggcgtggcgt ggtcggtgct ctactcgttc ttcgacgagg tcgacctctc
      541 acagtcgtac ggcgcgccgc ctcagccggg ctacgtcgag gacctgctct cggagctcga
      601 cctggtggag gaggagatcg ccacacatca tgcgggggcg gcgacggtcg cccgcaaccc
      661 ggccgagctc gacgcggcgc tcgtggccgg ccaggtggcg ctcgtgcact gcgtggaggg
      721 cggctaccac ctcggcgcca cgccgcaggc gatcgacgca gcggtcacgc agatcgctcg
      781 ccggggcgtc gcctacgtga tcctcgcgca cctgttctgg cgcagggtgg cgaccaacgc
      841 gaacgcggtc ccgttcatcc ccgacccgat ctaccggttt gtgttcccgc agccgagcgt
      901 cggcctcagc gagctgggcg aggccgccgt gcgggccatg gtgcgcgagc gcgtgctgat
      961 cgacgtgtcg cacatgagcg agcgcgcgcg ggcgcgcacg ctcgagctgc tcgacgagat
     1021 cgacccggcg aagtccacgc cgctgctcgc ctcgcacgcc ggctaccgct tcggcaagca
     1081 ggagtacaac ctcaccgacg acgaggtgcg cgccatcgcc gcgcgcgacg gcgtgatcgg
     1141 gctgatcttc gcccagcacc agctgctcga cggcctgcgc aagcggacga cgacgttcga
     1201 tgagtcgttc gaggcgatct gcgcgcacat cgacaagatc cgagacctca ccggatcaca
     1261 ccgccacgtg ggcctcggct cggacttcga cggcttcatc aagcccacac tcggcgggct
     1321 cgagagcgcc gcggacatgg cacagctcga ggctgcgctc gtgagccact acggcgccgg
     1381 cgacggcgcg gccatctgct cggggaacgc gctacgactg ctgcgcagct actggcgtgg
     1441 caagccggcc tagctcgtcg ccacgatcat cacgtcgcag ttcgcgtgat gcgagagctt
     1501 gttcggcacg ctgccgagca tgcggcgcgc gccggtcata ccgcgattgc caacgaggat
     1561 gaggtcggcg ttctcctcgt cggcgacgtt gaggagcgcc tcggccgggt cgccgttcac
     1621 ggcgtgacag tgcacgtcga ggccggcgtt gcgcatccgc accgcagcct gctcgagcac
     1681 ggagtccgcg tgcatgctgc gctggacggt ccactgggct gcctccggcc cgggctcgcc
     1741 gcccacgcgc gcgcctgaca tcggcttgta ggcgcttacg agatgcacct tcgcgcctag
     1801 cgcgtttgcc agctcggccg ccctctgcgc ggcgcgcgcc gcagtatccg agccatcgat
     1861 tccgacaacg atcgaaccga tcatctccac cccctctgtc gcgagtactg ggagcgatcg
     1921 taaacccgct cggggcgtca gacgaccttc cagggctcca cgcgccgtgt ggtcactagt
     1981 ccgttcgcga cgtacgggtc ctcggcggtg aactcatcga cctcgcccgg gtcgtccacg
     2041 cggaaaacga gcgcgccgcc gtagggcggg tcgccgagtg cgcccgccag ctccaggcgg
     2101 ccgctctcct tccagcggcg gatcaacgcc aggtgcgcgt cgcggtacgg cgctcggcgc
     2161 tccacgatgt ccggcacgta ctcgtacagg agcatccagt acggcttgtc gtcgctcatc
     2221 ggcggcacgg tacctcaggc ggccgctgcg gctgcgacat ctgcggtgac gcgatcgccg
     2281 tcgtaggcgt acagctcggg gaagaggacg gcgatcaggc cgaggcccgc gacgcaggcg
     2341 agcccgccgg acaccaccga gaagcgcggc gaagcgaccg atgcgaccgt gccggactcc
     2401 acgtcgccga gccgcggtcc gcccgccacc accaagctga acaccgccga catgcggccg
     2461 cgcatcctgt cgggcgtgag cgtctgcgcg atcgtcgagc ggcacactgc gctcacgctg
     2521 tccgcggcgc ccgcgattgc gaaaagcacc agcgccggcc agatcgtggt catgagaccg
     2581 gccgccccga cggcgaggcc ccagacgccg acggcgccaa acacgatccg gccgaggtac
     2641 cgcgcctggc caagccagcc ggttgtgagg gcggccacgg tcgcacccgc cgagaccgct
     2701 gcgtagaggc ctccggtccc ggtcgcaccc gcgtggtaga cgctgagcgc gaggactggg
     2761 aacagggcgc ggggcatgcc gaaggtcatc gcgaggaggt cgatcgcaaa gctgcccatc
     2821 accgcgcggt cgccgcgcac gaagcgcagg cccgtggcga tcgagcgccc gatcggctcg
     2881 tgctcctcga cgccgatcgg cgggcgcggg ctcatggcgg ccgccgcgag cgccatgccg
     2941 aggcagctga ccgcgtcgac cgtgtaggcc gcgccgacgc cgagccagga gatcagcagg
     3001 ccgccgaggg ccgggccgac caccatcgtg agctgcatca ggccgaagtt ggtcgagagc
     3061 gcggcgcgca ggttctccgg cgacgcgagg ttcggcacca tcgactggcg cgccacgcgc
     3121 tcgagctggc ccgcgcccgc catcgccccg gcgagcacgt acagcagcca gaccggcgga
     3181 ttgccggcga acgcggcggc ggccagcgag cccgccactg ccacgagcgc gagttggcag
     3241 agcagcagca tggtgcgccg gtcgtgccgg tcggcgagcg cgccgccaat cagcgacgcg
     3301 gccaccagcg ggccgagctc cacgaccccg agcagaccga cgagcagcgg gtcgtgcgtg
     3361 agcacgtaca cctgataggg cagtgccacg agcgcggcct gcgtgccgag ccccgtgacg
     3421 agcgcgcctg cgctcagcag gcggtagtca cgcgactcgc ggaagggcgc gagatcgggc
     3481 gtgagggatc gcagcagcga gcgcaccacg caggcgaagc tagcgcgggt cgcgccggcg
     3541 cagcgagggg tcggcgatcg ccggcggctc gtagcccgcg tccgcggggt tgacgttcgt
     3601 gcccgggggc acgatctcgt cgaccttgtc gagcacctcg ggcggcagcc tcacgtccgc
     3661 cgccccgagc tggctctcga gctgctcgat cgtgcgcggc ccaatgatcg ctgcgctcac
     3721 cgcggggtgc tgaaggacga acgcgatggc caggtggacg aggctcaggc cctgctgctc
     3781 ggcgagccgc aacagggcgt cggcggcatc cagcttgcgc tggttctcgg gcttcgagag
     3841 gtcatagcgc gccgggagtc gctgggcgcg cgtgctcgtt ggggcctcca cgcccttccg
     3901 ccacctcccg gacagccaac cgcccgcaag cgggctccac gggatcacgc cgaggccgta
     3961 ctgctggcac acgggaagaa cgtccttctc gattccgcgc acgagcagcg aatagggcgg
     4021 ctgctcgctc gcgaagcgct cgcgcccacg gcgctccgcg acgcactgcg cctcaacgat
     4081 cgcgtgggcg gggaaggtcg agtgcccgag gtagcggatc ttgcccgcgt gcaccaaatc
     4141 ggtcaaagcg ccgagcgtct cgtcgatgtc ggtcgagggc tccgggcggt gcacctggta
     4201 gaggtcgatc cagtccgcct gcagccgcct caggctgttg tcgacctcct gcatgatcca
     4261 gcggcgcgag ttgccgcgct cgttcgggtc ctcccccatc ggcccgtgga acttcgtggc
     4321 gagcacgacg tggtcgcgcc tgccgccggc gagcgccttg ccgacgatct cctcggactc
     4381 gccgccggag tagacgtcgg cggtgtcgac gaagttgatg ccggcgtcga gcgccgcgtg
     4441 gatgatccgg atcgactcgt cgtggtcgcg gttcccccac cgcccgaaca tcatcgcccc
     4501 gaggcacagc gggctcacgt tcactccggt gcgtccaagc atgcggtgct ccatgccggc
     4561 ggatcctacg gagtggcgct gcccggccgg cgggcgtagc ttcgtgagag cggacacgcc
     4621 cctgggcagg gacgctcgct ctcgattgga gggtcacatg tcaggactcg cacgactccg
     4681 gcgcgggctc gttcccgccc tggtcgcact gctggcgctg ctctgcgcca cgagcgcggt
     4741 tgccgccacg aggaagagcc acaaggtgaa cgcgacgatc actgcagcac ccctcacggc
     4801 cgccaacggc gtgacgactg tcaccgggat cgtcaccggc aagccattcc ccggcgaggg
     4861 cgcggttgtc taccgcgtga agactggcgc gacgggaacc atcacggcga acgtcaccgt
     4921 cttcacgcgc aacggcatcg tgaagggcag cactgtcgct accgtcacgc ccggcgcgaa
     4981 caacacgtcc actctcacgg gcaccgccaa agtcgccagc ggaaccggca actttgcggg
     5041 tgcaacgggc aaggacctga aggtgaccgg caccatcgac gccgacggcg ttctgcacgt
     5101 gaacgtgacc ggctcgatca agtactgagc cgcgccgcgg gcgcgcggcc acggtcgcgc
     5161 gcccgtcttt cctgcgggcc ggcccccaac gccgcgtgct cggctaccgt ccctgcggat
     5221 gagtgacgca cgccgtccct cgctcgacgt cgcccgcatc ctcgaggagc gagcgggcga
     5281 gcagctcgcg ctgaacgacc ggcacctgaa tccgcagttc gggcgggttc tgcggacgat
     5341 cggctacgac cgcaactgga agcacgcgag cggcgcgtac ctcttcgacg agaatggcga
     5401 ccgctggctg gacatgctcg gcggctacgg cgtattcgcg ctcggccgca acaacgcggc
     5461 cgtgcgccgc cagctcgagc gcttcctcgc gaccgagacc gccacgctgc cccagctcgg
     5521 ggtcactctg ctggcgggcg tgctcgccga gcagctgctc gcgcggtcgc cgtcgcggct
     5581 cggcgccgtg ctcttcacca actcgggaac ggaagccgtg gagggcgcca tgaagctcgc
     5641 gcgcgcggcc accggccggc cgcgcatcgt gtactgcgac cacgctttcc acgggctcac
     5701 gctgggatcg ctgtcggtga acggcaacga ggaattccgg gagcgcttcg ggccgctgct
     5761 gcccggctgc gaccccgttc cgttcggaga cctcgacgcg ctgcgttcgg agctcgcccg
     5821 cggcgatgtg gccgcattcc tcgtcgagcc ggtgcagggc aagggcgtcg tgatcccgcc
     5881 cgacggctac ctgcaggagg cgcaggagct ctgccgccgc cagggcacgc tgtgcatcgc
     5941 ggacgaggtt cagactggcc tcgggcgcac cggtcgcttc ctcgcgctcg agcactgggg
     6001 actcgagccc gacatggtgc tcgtctccaa ggcgctctcc ggcggctacg tgccggtcgg
     6061 cgcggtgctc gccgcgcgca gcgtgttcga gcgcgtgttc gacacgatgg agcgcgccgt
     6121 cgtgcacggc tccaccttcg gcaccaacga tttcgcggcc gccgccggga tcgcgacgct
     6181 ggccgagctc gacgacgccg acctcgtcga gcgctcgcgc cgcagtggcg agcggttgct
     6241 cgagctcacg cgaccgctcg tggagcgcta tgaggtggtg cgggacgtgc gcggcatcgg
     6301 cctgatgtgg gcgatcgagt tcggaccacc cgccggcggc gccgcgcttc gggcctggaa
     6361 ggtcgccgag tcgcgacagc ccgggatctt cgcccagctc gtgaccgtgc cgctcttcaa
     6421 cgagcaccgc atcctcacgc aggtcgccgg ccaccgcatg aatgtggtga aggcgctccc
     6481 gccgctcgtg atcgaggacg atgacctcgt gcacttcgcc gactccctcg accaggtgct
     6541 agctaagctg gagcgcggcc ccgcgcgtgc gttcgctcgc ttcggactcg acgcagcgcg
     6601 ccgctcactg cgaccgtcgc gcgcctgact ctcgatgacc cccgcccctc ttatctgggt
     6661 atgacagtct cggatgcgag atttgcgacg acggctgcgt gaacatccct acgcgctgac
     6721 cgcgctcgcg ctcgcgttcg ccgccaccgc ggtcgcgggc atcgccaacg cgtacgggtt
     6781 cggggcggtc gacagcgcct tcgacgcgat cgagcccggc tggatcgcgt tcatcgccgt
     6841 ggccgagatc gtctcgtacg gcgcgtacgt ggtcgcgtac cgctcgatcg cctgcgtgca
     6901 gggacacgag ccgattccgc tgcggctggc agcgcgggtg gtggccgcag gcttcgggcc
     6961 gctctcggcc ggcggcggct tcgcgctcga taagcaggcg ctgcacgcgc tgcactccga
     7021 tgagcgcgcc gcgaccgtgc gcgtggttgg cctcggggtg ctggagtggg cgctgctcgc
     7081 gccggccgcg tctatctgcg cgatcgcgat gctgctcggc aaccgcgacg gcgtatggcc
     7141 gtcgctcctg tggccgtggg cgctcgccgt gccggcgggg ttcgcgctcg ggctgtttgc
     7201 ttccgcgccg ggcacgcgca ggcgcatcct caggtggttc gggaacccgg gctggctcgg
     7261 ccacctgctc gagggagtcg ccacgctgca cacg
//
LOCUS       DASVXS010000126         5481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1165884, whole genome shotgun sequence.
ACCESSION   DASVXS010000126 DASVXS010000000
VERSION     DASVXS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165884"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..920)
                     /locus_tag="VF752_06945"
     CDS             complement(93..920)
                     /locus_tag="VF752_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8855322.1"
                     /translation="MRRLVRAWLRRTPLLPRRVRVALAVACAAGAVAVVAFAPQRSQA
                     QSQRPPSVPPTQTRVDPALAPEGRALFVDGCSSCHGLDARGIKGTAPSLVGVGPGPVD
                     FYLRTGRMPLADPRDQPQRGRPIYQASEISAIASYVASLGGSQQLPRANPAAGSLSQG
                     FSLFTEHCAGCHQVVAQGGLTIEAQVPSLQKSKPTDIAEAVRMGPYLMPKFPPSQINQ
                     HQLDSIARYVVYTRDPDDRGGWSIGHIGPIPEGMVAWLLAGAALVLVIRLIGERNEI"
     gene            complement(917..1750)
                     /locus_tag="VF752_06950"
     CDS             complement(917..1750)
                     /locus_tag="VF752_06950"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8855323.1"
                     /translation="MSQPTLGRILLDWQLVPGVVILCALCAGLYVAGVWRARRWPAWR
                     TGCFLIGVALIAFALCSGLDAYADTLLSIHMGQHLVLSMAAAPLLVAGSPLVLALRAL
                     PASSSLRRGLVVLIHSRAFAILTSAPVAWLAFSATMLLTHFTGLYSLALRDPAVHALE
                     HTLYLVTGVLFWWPLLDAGPLPGRQLGVVGRCAYLLLAMPVMAFIGAVMNWSDRLLYP
                     EYAAPARALGRSALADQHNAGALMWVGGKLVMALLLLVTAWAALQREEKRQRAREARA
                     A"
     gene            complement(1747..2142)
                     /locus_tag="VF752_06955"
     CDS             complement(1747..2142)
                     /locus_tag="VF752_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855324.1"
                     /translation="MRRAAIPLLAWAVLLTCSAVGLWIYSHSWLNATLISAAAALTYL
                     IALGFVIRHRRMPDASEARAHPELSVRALPDLSFGSALIGIAIATMIFGAEFGLFLVY
                     IGLGLLLFGVARLIFELRAERRALEEARR"
     gene            complement(2139..3770)
                     /locus_tag="VF752_06960"
     CDS             complement(2139..3770)
                     /locus_tag="VF752_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8855325.1"
                     /translation="MSTIAARRRAPAWTSVIGATDHKTLGRNLLIAAFAFFIAGGVLA
                     LLMRLELAAPGMQFVSHDTYSELFTMHGSTMIYLFVTPLALALGVYLVPLQIGAADIS
                     APRLANVGFWLYITGGLTLESGFLTAQGPGKAGWFATWPLSTSGATPGTGMNLWVLGV
                     IMSALGMIAIGVCLLGTIMRRRAPGMTLLRMPPFTWTQLVTALMVIGGFPVLVLVMTL
                     LYLDRQGVHIFNGFEGAIDYQNLFWFYGHPVVYVMFFPFVGCALEAIATNTGRRLFGY
                     RLFALSLLLFTALSMSVWAHHMYTTGGITNRYFAFTSTALLVPAGIEYFDMLGTLIGG
                     SIIYRTSLLFGVTFFLQFLVGGASGIFVASPTLDYHAHDGYFVIAHFHYTLFAGSIFG
                     FFAGVYHWFPKAFGVTLREGLGKLHLVLLIIGTNMTFFPMFFLGQEGMPRRVATYPRS
                     TGWETLNTVETVGAFIIALAIVVFLWNVVVSLRSHVAAGPDPWDGQSLEWATSSPPPR
                     HNFDVLPPVRSYAPLLDLKKAGEWPRRRELEETPA"
     gene            complement(3767..4396)
                     /locus_tag="VF752_06965"
     CDS             complement(3767..4396)
                     /locus_tag="VF752_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8855326.1"
                     /translation="MVDTGHEYHHLFSIYVPIAVGVWLAIALATGYGLLRYRRRPGRV
                     PSGRHEAQVPELTYAALLVGVAAMLLYFTFTTESKVDALPRKPGLVLHVTAGQWNWRF
                     NYPNGASVVSSSLHQRVMQVPTNTVILVKLHSVDVVHSLWIPERRIKKDAFPGADNEL
                     KLTFPKAGTFSGACAEFCGLHHAEMRFGVKAVPRPQFDAWLRVQAGASG"
     gene            4536..>5481
                     /locus_tag="VF752_06970"
     CDS             4536..>5481
                     /locus_tag="VF752_06970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8855327.1"
                     /translation="MLGRFSLLSVLAFLILGAALSHLLSSQIRSRALANATQSASRIS
                     RFGIQPQLSGVDLNQPLSPEAVETLDDLLRAGYTSEDVDTIAIWNRRGRVVYSNDHGL
                     IGRTAPGDARLAKALAGRSHACERQSGAKLIEVFVPLHTNAGTPPTGAFEIYLHSDRV
                     AAAVKRDTRRVYLVLSAGLLLLWAAMFRIAARASARLRRQAAENDFQAHHDPLTSLPN
                     RAAFIAQVDQAIELVDPELSTAVLAVDLDRFKEVNEALGYQSGDGLLRQAAERLLISV
                     RSSDTVARLGNDEFAVLLPRVTPAEAETVATRARAALEE"
BASE COUNT          953 a         1877 c         1928 g          723 t
ORIGIN      
        1 cgcgggcggc gcgccagacg gctgcactgg ctgcttcagg cgccggccca ggaagaaggc
       61 cacgagcagt tgccagatcc gccgcaggag cctcatattt cgttgcgctc cccgatcagg
      121 cggatcacca gcacgagcgc cgccccggcc agcagccagg ccaccatgcc ctccgggatg
      181 ggcccgatgt gcccgatcga ccagccgccg cggtcgtcgg gatcgcgcgt gtagacgacg
      241 tagcgcgcga tcgagtcgag ctgatgctga ttgatctggc tgggcgggaa cttcggcatc
      301 agatacggtc ccatccgaac cgcctcggcg atgtcggtgg gcttggattt ctgaagcgaa
      361 ggcacctgcg cctcgatcgt gaggccgccc tgcgccacca cctggtggca gcccgcgcag
      421 tgctctgtga acaggctgaa gccctggctg agcgagcccg ctgccgggtt ggcacgcgga
      481 agctgctgcg agccgccgag cgaggcgacg tacgacgcga tcgcggagat ctcactcgcc
      541 tggtagatcg gcctgccgcg ctgcggctgg tcgcgcggat cggccagcgg catgcggccg
      601 gtgcggaggt agaagtcgac cgggcccgga ccgaccccaa cgagcgacgg agccgtgccc
      661 ttgatcccgc gggcgtcgag cccgtggcag gaggagcacc cgtccacgaa cagcgcgcgc
      721 ccctcaggcg ccagggcggg atccacgcgc gtctgcgtcg gcggcacgct cggggggcgc
      781 tgcgactgcg cctgggagcg ttgcggagcg aacgcgacga cggcgaccgc gccggccgca
      841 cacgcgactg cgagtgccac ccttactcgc cgaggcagca gcggcgtgcg gcgcagccag
      901 gcacggacga gacgcctcac gccgcccgcg cctcccgggc gcgctggcgc ttctcttcgc
      961 gctgaagcgc agcccacgcg gtcacgagca gaagcaacgc catcacgagc ttgccgccca
     1021 cccacatgag cgcgccggcg ttgtgctggt cggcgagcgc cgagcggccg agcgcgcgcg
     1081 ccggcgccgc gtactccggg tagagcagcc ggtcgctcca gttcatcacg gcgccgatga
     1141 acgccatcac cggcatggcg aggagcaggt acgcgcatct ccccacgacc cctagctgcc
     1201 tgccgggcag cgggcccgcg tcaagcagcg gccaccagaa cagcacgccc gtgacgaggt
     1261 agagcgtgtg ctcgagcgcg tgcacagccg ggtcgcgcag cgcgagcgag tacagcccgg
     1321 tgaagtgcgt gaggagcatc gtcgccgaga aggccagcca cgcgaccggc gcgctcgtga
     1381 ggatcgcgaa cgcgcggctg tgaatcagga ctacgagtcc tcgccgcagg ctcgagctcg
     1441 ccggcagcgc cctcagcgcg agcacgagcg gagagcccgc cacgagcagc ggcgccgccg
     1501 ccatcgacag cacgaggtgc tggcccatgt ggatcgaaag caacgtgtcc gcgtatgcgt
     1561 cgaggcccga gcagagcgcg aaagcgatca gggcgacgcc gatcaggaag cagccggtcc
     1621 gccaggcggg ccagcggcgc gcgcgccaaa cgccggccac gtagaggccc gcgcacagcg
     1681 cgcacaggat cacgacgccg ggtacgagct gccagtcgag caggatgcgg ccgagcgtcg
     1741 gctggctcat cgccgcgcct cctccagcgc gcgccgctcg gcgcgcagct cgaatatcag
     1801 ccgcgcgacg ccgaacagca gcagccccag gccgatgtac acgagaaaga gcccgaactc
     1861 cgcgccgaag atcatcgtgg cgatcgcgat gccgatcagc gcggacccaa agctcaggtc
     1921 tggcaacgcg cgcacggaga gctcaggatg cgcgcgcgcc tctgacgcat cgggcatgcg
     1981 ccggtggcgg atcacgaacc cgagcgcgat caggtacgtg agggccgccg cggccgagat
     2041 cagcgtcgcg ttcagccacg agtggctgta gatccacagt ccgacggccg agcaggtgag
     2101 cagcacggcc cacgcgagaa gcggtatcgc cgcgcgcctc atgccggcgt ctcctcgagc
     2161 tcgcgcctgc gcggccattc ccccgccttc ttcaagtcca gcagcggcgc gtacgagcgc
     2221 accggcggca gcacgtcgaa gttgtggcgc ggcggcggcg agctcgtggc ccactcgagc
     2281 gactggccgt cccacgggtc ggggcccgca gcgacgtggc ttcgaagcga caccaccacg
     2341 ttccacagga acacgacgat cgccagcgcg atgatgaacg cgcccaccgt ctcgacggtg
     2401 ttgagcgttt cccagccggt cgagcgcgga taagtggcca cgcgtcgcgg catgccctcc
     2461 tgcccgagga agaacatcgg gaagaaggtc atgttcgtcc cgatgatgag cagaacgagg
     2521 tgcagcttgc cgaggccctc gcgcagcgtc acgccaaacg ccttcgggaa ccagtggtac
     2581 acgcccgcga agaaaccgaa gatgctgccc gcgaacagcg tgtagtggaa gtgcgcgatg
     2641 acgaagtagc cgtcatgcgc gtggtagtcg agcgtcggcg aggcgacgaa gatgccggag
     2701 gcgccgccga cgaggaactg caggaagaac gtgacgccga acagcagcga ggtgcggtag
     2761 atgatcgagc cgccgatgag cgtgccgagc atgtcgaagt actcgatgcc ggcgggcacg
     2821 agtagcgcgg tcgaggtgaa cgcgaagtag cggttggtga tgccgcccgt cgtgtacatg
     2881 tggtgtgccc agaccgacat cgacagcgcg gtgaacagca gcagcgagag cgcgaacagc
     2941 ctgtagccga acagccgccg cccggtgttc gtggcgatcg cctcgagggc gcagccaacg
     3001 aacgggaaga acatcacgta gacgaccggg tgcccgtaga accagaacag gttctggtag
     3061 tcgatcgctc cttcgaagcc gttgaagatg tggacgcctt ggcggtccag gtagagcagc
     3121 gtcatcacga gcacgagcac cgggaagccg ccgatcacca tcagcgcggt cacgagctgc
     3181 gtccatgtga acggcggcat gcgcagcagc gtcatcccgg gcgcgcgccg gcgcatgatc
     3241 gtgccgagca ggcagacgcc gatcgcgatc atcccgagcg ccgacatgat cacgccgaga
     3301 acccacaggt tcatgcccgt gccaggcgtg gcgcctgagg tcgatagcgg ccaggtcgcg
     3361 aaccagcccg ccttgccggg gccctgagcc gtgaggaagc ccgactcgag cgtgaggcca
     3421 ccggtgatgt agagccagaa gcccacgttc gcgagccggg gcgccgagat gtccgcggcg
     3481 ccgatctgga gcggaacgag gtacaccccg agcgcgagcg ccagcggcgt cacgaacagg
     3541 tagatcatcg tggagccgtg catcgtgaac agctcgctgt aggtgtcgtg cgagacgaac
     3601 tgcatgccag gcgcggcgag ctcgaggcgc atgagcagcg cgagcacgcc gccggcgatg
     3661 aagaacgcga aagctgcgat cagcaggttg cgaccgagcg tcttgtggtc ggtcgcgccg
     3721 atcaccgacg tccacgctgg ggcccgccgg cgggcggcga tcgtgctcat ccgctagctc
     3781 ccgcctggac tcgcaaccag gcgtcgaact gcggccgcgg cacggctttc acgccgaagc
     3841 gcatctcggc gtggtgcagg ccgcagaact cggcgcacgc gccgctgaac gtgccggcct
     3901 tcggaaaggt cagcttgagt tcgttgtcgg cgcccgggaa ggcgtccttc ttgatgcgcc
     3961 gctctgggat ccagagcgag tggacgacgt ccacggagtg cagcttcacg aggatcacgg
     4021 tgttcgtcgg cacctgcatc acccgctggt ggaggctgct cgacacgacc gacgcgccgt
     4081 tcgggtagtt gaagcgccag ttccattggc cggccgtcac gtggaggacg aggcccggct
     4141 tgcgcggcag cgcgtccacc ttgctctcgg tggtgaacgt gaaatagagc agcatcgcgg
     4201 cgacgccgac gagcaacgcc gcgtacgtga gctcgggcac ctgcgcctcg tggcgcccgc
     4261 tcggcacgcg accggggcgc cgccggtagc gcagcaggcc gtagccggtc gcgagggcga
     4321 tcgcgagcca cacgccgaca gcgatcggca cgtagatcga gaacaggtgg tggtactcgt
     4381 ggcccgtgtc gaccaaggag agcctcggct ggccgcttac cccaggggtt acgcagcgaa
     4441 acggcgcctc aaccgatggg catcggtgcc gatcaaccgg gcagcatggc caggactttc
     4501 gtcccgcgtg cgcgccgggg ctcgccggtc tggggttgct cggcaggttc agcctgctga
     4561 gcgttctcgc cttcctgatt ctcggcgccg cactctcaca tctgctttcg agccagattc
     4621 gctcgcgcgc gctcgcgaac gcgacacagt ccgcctcgcg catctcgcgc ttcggcatcc
     4681 agccgcagct gagcggcgtc gacctgaacc agccgctcag tcccgaggcg gtcgagaccc
     4741 tcgacgatct cctgcgcgcc ggatacacga gcgaggacgt cgacaccatc gccatctgga
     4801 accggcgcgg gcgtgtcgtc tactcgaacg accatggcct gatcggccgc accgcgccgg
     4861 gagacgcccg gctggcgaag gctcttgccg gccgctcgca cgcctgcgag agacagtccg
     4921 gcgccaagct gatcgaggtc ttcgtcccgc tccacacgaa cgccggcact ccgccgacgg
     4981 gtgcgttcga gatctacttg cactccgacc gagtcgccgc cgcggtgaaa cgcgacacgc
     5041 ggcgcgtcta cctcgtgctg agcgccggcc tgctgctctt gtgggcagcg atgttccgca
     5101 tcgccgcgag ggcctcggca cggctgcgcc gccaagccgc cgagaacgac ttccaggcgc
     5161 atcacgaccc cctcacctcg ctgcccaacc gcgcggcgtt catcgcccag gtggaccagg
     5221 ccatcgagct ggtggatccg gagctctcga cggcggtgct ggcggtcgat ctcgaccgct
     5281 tcaaggaggt gaacgaagca ctcggctacc agagcggtga cggcctgctg cggcaggcgg
     5341 ccgagaggct gctcatctcg gtgcgctcga gcgacacggt cgcccggctc ggcaacgacg
     5401 agttcgcggt gctgctgccg cgcgtgacgc ccgccgaggc ggagacggtc gcgacgcggg
     5461 cgcgtgccgc gctggaggag a
//
LOCUS       DASVXS010000127        11876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_87488, whole genome shotgun sequence.
ACCESSION   DASVXS010000127 DASVXS010000000
VERSION     DASVXS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87488"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..723
                     /locus_tag="VF752_06975"
     CDS             241..723
                     /locus_tag="VF752_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX8855328.1"
                     /translation="MGGSFEKLMGELDYPLYVLTAAAGDQRSGCLIGFATQTSIHPPR
                     FLACVSRKNHTHPVAVRSEVLAVHVLRDEHRDLAELFGGETGDEVDKLAHVCWRPGPG
                     GVPLLADVSAWFAGRVIGRFDLGDHEGFLLEPLEASAAPLPSELSFQEAKDIEPGHEP
                     "
     gene            1023..2378
                     /locus_tag="VF752_06980"
     CDS             1023..2378
                     /locus_tag="VF752_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855329.1"
                     /translation="MDRTEADVVVVGAGLAGLAAARELVRAGREVIVLEARDRVGGRT
                     LNHDIGDGKVVEVGGQWIGPTQDRLAELARELRVGTYSTYDEGENVIEFRRTLKRYRG
                     AIPRINLAVLLDVGQAQARLDRMARQVPLEAPWEAPKAAEWDSQTLWSWMQRNVATSG
                     ARELLTIATEAVWAVEPADVSLLHFLFYTHSAGKFDNLVGTSGGAQQDRFEGGSQLLS
                     IRLAEQLGERVRLSTPVRSIGHGDGGVEALAGELRVRARRAVVAIPPTLTSRIAYEPA
                     LPGHRDQLTQRIPQGTVIKCMALYERPFWRERGLTGQATSTRGPVKVTFDNSPPDGSP
                     GVLLGFLEGRAARQLGLATPADRRAAVVDCFTRYFGAEAARPVDYVERAWAEEEWTRG
                     CYGCYMPTGVWTSYGHALRAPVGPIHWAGAETATVWSGYMDGAVQSGLRAAAEVLEAL
                     S"
     gene            complement(2602..3159)
                     /locus_tag="VF752_06985"
     CDS             complement(2602..3159)
                     /locus_tag="VF752_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855330.1"
                     /translation="MKSTDPLHYGWWLGSRAAGIVGFGLAALAVILGLAMATGIRLPK
                     QLRGRALHEQIALASLAAIALHGALLLGDRWLKPGLAGILVPFAGGYRPFYTGLGILG
                     AYVAAIVGLSFYVRRRFGTQRWRKLHRFAPLVYVLGLIHTLGAGSDAGTAWMRAIVLV
                     SAWPIASLLVLRLLTPPRGAAAQPQ"
     gene            complement(3156..4142)
                     /locus_tag="VF752_06990"
     CDS             complement(3156..4142)
                     /locus_tag="VF752_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX8855331.1"
                     /translation="MSAVDHSFRCMGTTVRLILDDRGRRDRSRLPLEDECKLAVAYLR
                     EFDARLSRFRPDSELSRLNADPRPAVPASQLLRVLVRAGVWAARRSGGLVDPTLTPDL
                     KRLGYSAPWPHPEAATVAEALAAAPRRAACAPSRERRWESLRANDRRGTVQRTPGVLF
                     DSGGVGKGLAADAVAVRLHALPRFLVDCGGDLVAGGRDDERAPFEVEVEHPLRRQPAH
                     RLTVRDGAVATSGIGRRVWRRTDGSHAHHLLDPSTGEPAWTGLVSATAIAATALEAET
                     VAKMAFLAGPERARTLLLAHGGVLVHDSGEVEVIEPLRLVAGGGERVEPVAA"
     gene            complement(4139..4411)
                     /locus_tag="VF752_06995"
     CDS             complement(4139..4411)
                     /locus_tag="VF752_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855332.1"
                     /translation="MESPKRRRRGPRPMWVWLSSFAVFVALFGFEAVRMADGHDPVIK
                     GTSNSATGTTQSSTAQPSTTEPNTTTQSGTPSQDYTPTTPPSSGSS"
     gene            4579..5169
                     /locus_tag="VF752_07000"
     CDS             4579..5169
                     /locus_tag="VF752_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX8855333.1"
                     /translation="MGKLDGKRIAFLVANEGIEQVELTEPWKAVELEGGKPELIAPES
                     GKAQAFNHLDKADQFDVDHAVSEVSASDYDGLMLPGGVANPDNLRMHDDAVKFARDFF
                     EQGKPVAVICHGPWTLVEAGVVRGRTITSWPSLQTDIRNAGGNWVDEQVVVDSGMVSS
                     RKPDDLQAFNEKMIEEFAEGVHAEQTPAGAGASRSS"
     gene            5258..6463
                     /gene="ugpC"
                     /locus_tag="VF752_07005"
     CDS             5258..6463
                     /gene="ugpC"
                     /locus_tag="VF752_07005"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX8855334.1"
                     /translation="MAEITLKDVTKRYPDGTEAVKHMNMDIQDGEFMILVGPSGCGKS
                     TALRMIAGLEEISEGEIDIGGERVNEFEPRDRDIAMVFQNYALYPHMTVRENMGFALK
                     LAKVEKDEIDRRVEEAARILDLTEQHERKPANLSGGQRQRVAMGRAIVRDPAAFLMDE
                     PLSNLDAKLRVQTRTELARLQERLGTTTIYVTHDQTEAMTLGDRVAVMRLGVVQQVGP
                     GQELYERPVNLFVAGFIGSPSMNFLPGVVEGDRVRLPMVEVPIPEDLRGKLRDGQEVF
                     VGVRPEDIEDAAVVGEEKRHHGVVFRTKIELLESMGAELYAHFSLGGDAGVEHEHLRE
                     LAADAGTSDVPSESDRGLAVARLDASANVPVGREAELWLDTTKLHFFDPESGRSLRAA
                     AEELTEVPA"
     gene            complement(6444..7286)
                     /locus_tag="VF752_07010"
     CDS             complement(6444..7286)
                     /locus_tag="VF752_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855335.1"
                     /translation="MPDTGFPISDAQADFGRARRRRVVAALADRLRGEPDDVRMILPF
                     DEVLAALGHAGSRDLGLQSIALDSIVGTADQRTGDFDRSFRPTSTRVRGRWERIALAE
                     RRGEAMPPIDVYRIGELHFVRDGHHRVSVARARRRTHIEAYVREVRTKIEPGPSMRVS
                     DLAFKGHERLFYERVPLPPEWRKRIDLPDYWGYANLAEAIEAWGFRASQEVGEFLNRE
                     EVARRWFVEEFEPVVEMLQEADLMGDLSEAQAYMRVSAERYRLMRTHRWSDEAIERVR
                     RELR"
     gene            complement(7286..8314)
                     /locus_tag="VF752_07015"
     CDS             complement(7286..8314)
                     /locus_tag="VF752_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855336.1"
                     /translation="MRPDVARATLRELAALQADREDPETDEEPGKIVHEYRPNAEQRF
                     VDMGWPVRDGELRYYGSADSTSWFLVVLDALGDAQLARELEPNWRAAAAWLERSLERG
                     GGVVRWGPRRGSGGLIQQGWRDTTGPHAAYGGGILTPDGSVPEPPVADADVQAVAVAA
                     LRAVASLSGDPRWLELLERTRARVAELFDPETMAVDAHDRPVRGAGSQLGWLLWAGAL
                     PEDDVERFAERLTRPDILTEYGLRTLSSEHPQFAATAYHRGAVWPFDSWFGWGGLRAA
                     DREREAERVRDGVLDALDRLGQAPELYAVVAGQPERVPIANQLQAWTIGARWAFSVGY
                     DARPLQHE"
     gene            complement(8464..9366)
                     /locus_tag="VF752_07020"
     CDS             complement(8464..9366)
                     /locus_tag="VF752_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8855337.1"
                     /translation="MSTTVAQQRTWRARVGARARERAGPRPPRHRQWLLWLGVIATIA
                     FCLFPFYWMINTSLKTGAELNTGKLFPNNPSLDNYSSIFSNSAFTKALRNSAIVAGTS
                     TIIALTVGSFAAYALARLRFRAKFLILAVILSITTFPPIAIAAPVFKLWTDAGLFNTY
                     IGLILPFLTFVLPLTIYILVSFFRDIPKDLEEAALVDGATHFQAFRKIVLPLAAPGFV
                     TAGLLAFIFAWNEFLLSITLTSTPDRRPVSAAIAFFTGSSQFEVPLGTISAATVTITV
                     PLIVLVIIFQKRIVAGLTAGAVKG"
     gene            complement(9363..10301)
                     /locus_tag="VF752_07025"
     CDS             complement(9363..10301)
                     /locus_tag="VF752_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8855338.1"
                     /translation="MTTAEATVGRVRAPHRRRGLSERQLAGAMLSPSLIVIAVVAAYP
                     IGYAIWLSLNQYSVRVPGLSRWVGLDNYLDALKSSDFWNAFKTTFIFTGISVSLELVL
                     GLGMALLMHESFKGRGILRATVLVPWAVLTVGTAIMWRSIFEPDLGFANQVLDALGLP
                     GGHVVWLGQSGYALAVMIFADVWKTAPFMALLILAGLQVIPNDVYDAAKVDGATAWQR
                     FTRITLPLLKPAILVALIFRTLDALRIFDLPFVLTKGANDTETLSLQAYQTLTQDRLV
                     GQGSALAVLTFIIVMAVSLLYIRFVGGNIRTLSEDT"
     gene            complement(10309..11589)
                     /locus_tag="VF752_07030"
     CDS             complement(10309..11589)
                     /locus_tag="VF752_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8855339.1"
                     /translation="MNSKLSSGLAIAFVAAVAAGCGGGGGGGGGTTPSNVGENAKTVT
                     PGQAQNAKGNVNWCIGKDTTGAFSQVVKLHNQQNPNVKVKLTELPTAADEQRNQLIQR
                     LRAKSPECDVLGMDVIWTGEFAGQNWLLNVTPAINARKNEFIPSTINTAKIGDKYWAV
                     PFNTNAGFIYYDKSKVKTPPSTWQQTFSMAKSDGGFAFQGAQYEGLTVNFLEMLYAAG
                     GTVLNSDGSKSTVDSPQAEQVLTQMQNNIKDGAAPKANLTYMEEESKRAWLTDKDVLL
                     RNWPYVYAQAVAAKKNFGVTPLPKWQGGASASVIGGYNLGISAYSKNPGGALEFINFA
                     TAPAAQKKFFIKSSLPAVLTSVYTDPEVKKKVPFAAELLKGVQQGKARPVSPVYPQIS
                     EAIFKNVYSALQGTSPKSALQKMQSQINAALKTF"
BASE COUNT         1877 a         4055 c         4185 g         1759 t
ORIGIN      
        1 accccggcgc catctcgccg agcggtcagt accgcggcaa gtaccagttc agccgcgcca
       61 cctggcgcgc gatgggcggc acgggcgacc ccgcgacggc gcccgagtcg gttcaggacg
      121 ccatggcggc caagctgctc cgcacgagcg gcccgtcatc ctggccgaac tgcgcctagc
      181 cgaacacccc ttgcttcgcc ccgcgcggcc gcggccgcgc ggggcgtaag ctcgcccgcc
      241 gtgggcggtt ccttcgagaa gctgatgggc gagctcgact acccgctgta cgtgctcacc
      301 gccgcggccg gcgaccagcg ctctggctgc ctgatcggct tcgcgacgca gacgagcatc
      361 cacccgccgc gcttcctcgc gtgcgtttcg cggaagaacc acacgcaccc ggtagcggtg
      421 cgctccgagg tgctggcggt gcacgtgctg cgcgatgagc accgcgacct cgcggagctg
      481 ttcggaggag agaccgggga tgaggtcgac aagctggcgc atgtgtgctg gcgtccaggg
      541 ccgggcggtg taccgctgct cgcggacgtg agcgcgtggt tcgcggggcg cgtgatcggg
      601 cgcttcgatc tgggcgacca cgagggcttc ctgctcgagc cgctcgaggc gagcgccgcc
      661 ccgctgccga gcgagctcag cttccaggag gcgaaggaca tcgagcccgg ccacgagccg
      721 tgaagatctt tcggctaccc gcacttcaaa atgccagcta gctggtctgg tctactcacc
      781 agaccattgg caacattagt cgggcaatga gcgagccccg caccacctcc cagacaccgc
      841 cgtccacctc ctccggcacg gtgtccgggc agccggagag ccgctttcta ccgccgaccg
      901 cgctctcctc cccggcgcgc cggcggggca gttgtccgct aggcgagtag cggacagggg
      961 acgccgggcg cccactgggc gtccggcgtc ctcctcctag acgatccgga tacggtccgc
     1021 acatggaccg caccgaagcc gacgttgtgg tggtcggcgc cgggctcgcg gggctcgccg
     1081 ccgcgcgcga gctcgtgcgc gccgggcgcg aggtgatcgt gctcgaggcc cgcgaccggg
     1141 tcggcggccg cacgctcaac cacgacatcg gcgacggcaa ggtggtcgag gtgggaggcc
     1201 agtggatcgg cccgacacag gaccgcctgg cggaactcgc gcgcgagctc cgcgtcggga
     1261 cctactcgac ctacgacgag ggcgagaacg tgatcgagtt ccgccgcacg ctcaagcgtt
     1321 accgcggcgc gatcccgcgc atcaacctcg cggtcctgct cgacgttgga caggcgcagg
     1381 cgcgcctcga ccgcatggcg cgccaggtgc cgctcgaagc gccttgggag gcgccgaagg
     1441 cggcggagtg ggactcccag acgctgtggt cgtggatgca gcgcaacgtc gccacgagtg
     1501 gcgcgcgcga gctcctcacg atcgcgaccg aggcggtgtg ggcggtggag ccggcggacg
     1561 tgtcgctgct ccacttcctc ttctacacgc actccgcggg gaagttcgac aacctggtcg
     1621 gtacgagcgg cggcgcacag caggaccgct tcgagggcgg ctcgcagctc ctctcgatcc
     1681 ggctcgccga gcagctcggc gagcgcgtgc ggctgtccac gccggtccgt tcgatcggtc
     1741 atggcgacgg cggcgtggag gcgctggccg gtgagctgcg cgtgcgtgcg cggcgcgccg
     1801 tcgttgcgat accgcccacg ctcacgtcgc ggatcgccta cgagcccgcg ctgcccggac
     1861 accgcgacca gctcacgcag cgcatcccgc agggcacggt gatcaagtgc atggcgctct
     1921 acgagcggcc gttctggcgc gagcgagggc tgaccggcca ggccacgagc acgcgcgggc
     1981 cggtgaaggt gacgttcgac aactcgccgc cggacggctc acccggcgtg ctcctcgggt
     2041 tcctcgaggg ccgcgccgcg cgccagctcg gtctcgctac acccgcggac cgtcgcgcgg
     2101 cggtcgtgga ctgcttcacc cgctacttcg gcgcggaggc ggcgcgtccg gtcgactacg
     2161 tggagcgcgc ctgggcggag gaggaatgga cccgcggctg ctacggctgc tacatgccga
     2221 cgggtgtatg gacctcctac gggcacgcgc tgcgggcgcc ggtcggaccc atccactggg
     2281 ccggcgcgga gaccgcgacc gtgtggagcg gctacatgga cggggcggtg cagtcgggtc
     2341 tgcgcgccgc cgcggaggtg cttgaggcgc tcagctagcc cgggtggtag gtggcgatca
     2401 gcaccccggt cgtgctggta gtgctgtcga cggttcggcc acggcagcgg ctggcgcagc
     2461 gggaaaggtc tccagaacga cctttaagtt gagcaccatt taggtgctca atatcagtct
     2521 cgttcgaacc cgtcccccaa gtgctcgctg taagcgcttg cgccctaggc agcaatagcg
     2581 gagaccgccg aagaacgacg cctactgcgg ctgcgcagcc gcgccacgcg gcggtgtgag
     2641 cagccgcagc accagcagcg aggcgatcgg ccacgcgctc acgagcacga ttgcccgcat
     2701 ccaggcggtg ccggcatcgc tgcccgcgcc gagcgtgtgg atcaggccga gcacgtacac
     2761 gagcggggcg aaacggtgca gctttcgcca gcgctgagtg ccgaagcgcc ggcgcacgta
     2821 gaagctgaga cccacgatcg ccgccacgta ggccccgagg atgccgaggc cggtgtagaa
     2881 ggggcgatag ccgcccgcga acggcacgag gatcccggcg aggccgggct tcagccagcg
     2941 gtcgccgagg aggagcgcgc cgtgcagcgc gatcgccgcg agcgaagcca gcgcgatctg
     3001 ctcgtggagc gcgcggccgc gaagctgctt tggcagccgt atgcccgtgg ccatcgcgag
     3061 accgaggatc acggcgaggg ctgccagccc gaacccgacg atccccgctg cgcggctccc
     3121 gagccaccag ccgtagtgca gcgggtcggt cgacttcacg cagccaccgg ctccactcgc
     3181 tcgccgcctc ccgccacaag gcgaagcggc tcgatcacct cgacctcgcc ggagtcgtgc
     3241 acgagcacgc caccgtgagc cagcagcagc gtgcgtgcac gctcgggtcc tgccaggaag
     3301 gccatcttcg cgacggtctc ggcctcgagc gcggtcgcag ctatcgcggt cgccgatacg
     3361 agcccagtcc acgcaggctc gccggtgctg gggtcgagga ggtgatgcgc gtgagagccg
     3421 tcagtccgtc gccacactcg gcgaccgata cccgacgtgg cgaccgcgcc gtcccgcacc
     3481 gtcaggcggt gggcgggttg gcggcgaagc gggtgctcca cctccacctc gaatggcgcg
     3541 cgctcgtcgt cgcggccgcc cgccacgagg tcgcccccgc agtccacgag aaagcgcggc
     3601 agtgcgtgca ggcgcaccgc aaccgcgtcg gcggcgagtc ccttcccgac cccgccggag
     3661 tcgaagagca ccccgggcgt gcgctgcacg gtgccgcggc ggtcattcgc gcgcagcgac
     3721 tcccatctgc gctcccggct cggcgcgcag gcggcgcggc gcggcgcggc ggcgagcgcc
     3781 tccgcaaccg tggccgcctc cgggtgcggc cacggcgccg agtagccgag gcgcttgagg
     3841 tcgggcgtga gcgtggggtc cacaagcccg cccgaacggc gagccgccca cactcccgcg
     3901 cgaaccagca cgcgcaggag ctgcgaggcc ggcacggcag gacgcgggtc ggcgttcagg
     3961 cggctcagct cgctgtcggg gcgaaagcgt gagagccggg cgtcgaactc gcgcagatag
     4021 gcgacggcca gcttgcactc gtcctcgagc gggaggcgcg agcggtcgcg ccgaccacgg
     4081 tcgtcgagga tgagccgcac cgtcgtgccc atgcagcgga agctgtggtc gacggcgctc
     4141 acgacgagcc agaagagggc ggggtcgtgg gcgtgtagtc ctgcgagggt gtgccgctct
     4201 gggtcgtcgt gttcggctcg gtggtgctcg gttgcgcggt gctcgattgc gtggtgccag
     4261 tggccgagtt cgacgttccc ttgatgacgg ggtcgtgccc atcggccatg cgcacagcct
     4321 cgaagccgaa cagtgcgacg aacacggcga acgacgagag ccacacccac atcggccgcg
     4381 gccctcgtct gcggcgcttc ggggattcca tggcgggcat tcaaggccgc gcacatgaac
     4441 gagggcctag tggcgcgtaa gagttcatga agaccgggct gagctcggta ggcgcgcgcc
     4501 ccgcgccgga ttgacgccgg tttggcgggg cctacgcacg gtaagtaggg gcagagcgaa
     4561 gtagaaagga gaccgagcat gggcaagctc gatggcaaga gaatcgcttt cctcgtagcc
     4621 aacgagggca tcgaacaggt ggagttgacg gagccgtgga aggccgtcga gttggagggt
     4681 ggcaagcccg agctgatcgc gccggagagc ggcaaggccc aggccttcaa ccacttggac
     4741 aaggccgacc agttcgacgt cgaccacgcg gtgagcgagg tcagcgccag cgactacgac
     4801 gggctgatgc tgcccggcgg ggtcgccaac cctgacaacc tccgcatgca cgacgacgcg
     4861 gtgaagttcg cacgggactt cttcgagcag ggcaagccgg tggccgtgat ctgccacggc
     4921 ccgtggacgc tcgtcgaggc gggcgtggtg agaggccgca cgatcacgtc gtggccgagc
     4981 ctccagaccg acatccgcaa tgcaggcggc aactgggtcg acgaacaggt cgtggtcgac
     5041 agcggcatgg tctcaagccg caagcccgac gacctgcagg cgttcaacga gaagatgatc
     5101 gaggagttcg ccgaaggcgt ccacgcggag cagacgccgg ccggcgccgg cgcctcgcgg
     5161 tcgtcctagc agcacgtcag cggccggcct tcgggccggc cgcgacttga tctccgccgt
     5221 ggcgggtaat tgactcctgg gaaagagagg agagcccgtg gctgagatca ccctgaagga
     5281 cgtcaccaag cgctatcccg acggcacaga ggccgtcaag cacatgaaca tggacatcca
     5341 ggacggggag ttcatgatcc tcgtggggcc gtccgggtgt gggaagtcca ccgcactgcg
     5401 catgatcgcg gggctcgagg agatcagcga gggcgagatc gacatcggcg gcgagcgcgt
     5461 gaacgagttc gagccccggg atcgggacat cgcgatggtg ttccagaact atgcgctcta
     5521 tccgcacatg accgtgcgcg agaacatggg cttcgccctg aagctcgcca aggtggagaa
     5581 ggacgagatc gaccggcgcg tggaagaggc cgcgcgcatc ctcgacctca ccgagcagca
     5641 cgaacgtaag ccggccaacc tctcaggcgg tcagcgccag cgagtggcga tgggccgcgc
     5701 gatcgtgcgc gaccccgccg ccttcctcat ggacgagccg ctctcgaacc tcgacgcaaa
     5761 gctgcgcgtg cagacgcgta ccgagctggc gcgcctgcag gagcgcttgg gcaccaccac
     5821 gatctacgtg acgcacgacc agaccgaggc gatgacgctg ggcgaccgcg tcgcggtgat
     5881 gcgcctcggg gtcgtgcagc aggtggggcc gggccaggag ctctacgagc ggcccgtgaa
     5941 cctgttcgtg gccggcttca tcggctcgcc gtcgatgaac ttccttccgg gcgtggtcga
     6001 gggcgatcgc gtgcgacttc cgatggtgga ggttccgatc cccgaggacc tgcgcggcaa
     6061 gctgcgcgac ggtcaggaag tgttcgtcgg cgtccgcccg gaggacatcg aggacgcggc
     6121 ggtggtgggc gaggagaagc gacaccacgg ggtggtcttc cgcacgaaga tcgagctgct
     6181 cgaatcgatg ggcgcggagc tgtacgcgca tttctcgctc ggtggcgacg ccggggtgga
     6241 gcacgagcac ctgcgcgagc tcgccgccga cgccggaacc agcgacgtcc cgtcggagag
     6301 cgacagaggg cttgcggtgg cccgcctcga cgcttcggcg aacgtgccgg tcgggcgcga
     6361 ggccgagctc tggctcgaca ccacgaagct gcacttcttc gatccggaga gcgggcgctc
     6421 gctgcgcgcg gccgccgaag agctcaccga agttcccgcc tgacgcgttc gatcgcctcg
     6481 tcgctccagc ggtgagtgcg catcagccgg tagcgctcgg cgcttacgcg catatatgcc
     6541 tgcgcctcgc tcaggtcgcc catcaagtcg gcctcctgaa gcatctctac gaccggctcg
     6601 aactcctcca cgaaccagcg ccgcgcgacc tcttcgcggt tgaggaactc gccgacctcc
     6661 tgggaggcgc ggaagcccca tgcctcgatc gcctccgcca ggttcgcgta accccagtag
     6721 tcgggtaggt cgatccgctt gcgccactcg ggcggcagcg ggacgcgctc gtagaacagg
     6781 cgctcatggc ccttgaacgc gaggtccgag acgcgcatgc tcggccccgg ctcgatcttc
     6841 gtcctcacct cgcgcacata cgcctcgatg tgggtgcgcc tgcgggctcg cgccacagac
     6901 acgcggtggt ggccgtcgcg cacgaagtgc agctcgccga tgcggtagac gtcgatcgga
     6961 ggcatcgcct cgccgcgtcg ctccgccagc gcgatgcgct cccagcggcc gcgaacgcgc
     7021 gtgctcgtgg ggcggaagct ccggtcgaag tcgccggtgc gctggtcggc cgtgcctacg
     7081 atcgagtcga gcgcgatcga ctgcagcccg aggtcgcgcg agccggcgtg ccccagcgcc
     7141 gcgagcacct cgtcgaaggg caggatcatc cgcacgtcgt cgggctctcc gcgcaggcga
     7201 tcggcgagcg cggcgaccac cctgcgacgg cgcgcgcgtc cgaagtcagc ttgtgcgtcg
     7261 gatatcggga agccggtgtc aggcactact catgttgtag cggccgggcg tcgtatccaa
     7321 ccgagaatgc ccagcgcgcg ccgatcgtcc acgcctgcag ctggttcgcg atgggcactc
     7381 gctcgggctg accggcaacg accgcgtaca gctcgggcgc ctgcccgagg cggtcgagcg
     7441 cgtcgagcac gccgtcgcgc acgcgctcgg cctcacgctc gcggtccgcg gcacgcagcc
     7501 cgccccagcc gaaccaggaa tcgaatggcc acacggcgcc gcggtggtac gccgtcgcgg
     7561 cgaactgcgg atgctcgctc gacagggtgc ggagcccgta ctcggtgagt atgtccggac
     7621 gggtgaggcg ctcggcgaag cgctccacgt cgtcctcggg tagagcgccg gcccacagca
     7681 gccagccgag ctgcgagccc gcgccccgca ccgggcggtc gtgagcgtcc accgccatcg
     7741 tctccgggtc gaacagctca gccacgcggg cgcgcgtgcg ttcgagcagc tcgagccagc
     7801 gcgggtcgcc ggagagcgag gcaaccgcgc gcagcgcggc gaccgccacc gcctgcacgt
     7861 ccgcatcggc gaccggcggc tccggcacgc tgccgtcggg cgtgaggatg ccaccgccgt
     7921 aggcggcatg aggaccggtc gtgtcgcgcc atccctgctg gatcagtccg ccgcttccgc
     7981 ggcgcgggcc ccagcgcacg acgccccctc cgcgctcgag cgagcgctcg agccacgccg
     8041 ccgccgcccg ccagttcggc tcgagctccc gcgcgagctg cgcgtcgccg agcgcgtcga
     8101 gcaccacgag aaaccacgac gtgctgtcgg ccgacccgta gtagcggagc tcgccgtcgc
     8161 gcaccggcca ccccatgtcc acgaatcgct gctcggcatt cggccggtac tcgtgcacga
     8221 tcttgccggg ctcctcgtcg gtctccgggt cctcgcgatc ggcctggagc gccgccagct
     8281 cgcgcagcgt ggcgcgcgcc acgtcgggcc gcacgggcag gagctggagg gaggtgatca
     8341 gcgagtcgcg cccgaaaagc gcatggaagc gtccaggatc gcgggcgtcg accggttcgg
     8401 acgacgcgta cggccaaccc tccggcgaga ggacgcgatc gaggtcgctg acctcggcgg
     8461 cggctatccc ttcaccgcgc ccgcggtgag gccggccacg atgcgcttct ggaagatgat
     8521 cactagcacg atgagcggca ccgtgatcgt cacagtggcc gcgctgatcg tgccgagcgg
     8581 cacctcgaac tgagagctgc cggtgaagaa cgcgatcgcc gcggacacgg gtctgcgatc
     8641 aggcgtgctc gtcagcgtga tcgagagcag gaactcgttc caggcgaaaa tgaacgcgag
     8701 cagtcccgcg gtcacgaagc cgggcgcggc cagcgggagc acgatctttc ggaacgcctg
     8761 gaaatgggtg gccccgtcga cgagagccgc ctcctcgagg tccttcggta tgtcgcggaa
     8821 gaacgacacg aggatgtaga tcgtgagcgg caggacgaag gtgaggaacg gcaggatcag
     8881 accgatgtag gtgttgaaga ggccggcatc ggtccacagc ttgaacactg gcgccgcgat
     8941 cgcgatgggc gggaacgtgg tgatcgagag gatgaccgcg aggatcagga acttcgcgcg
     9001 gaagcgcagg cgcgcgagcg cgtacgccgc gaaggacccg accgtgagcg cgatgatcgt
     9061 cgaggtgccc gccacgatcg cgctgttgcg gagcgccttc gtgaacgcgg agttgctgaa
     9121 gatcgagctg tagttgtcga gcgacgggtt gttcgggaac agcttgccgg tgttcagctc
     9181 ggcgcccgtc ttcagcgatg tgttgatcat ccagtagaac gggaagaggc agaacgcgat
     9241 ggtcgcgatc acgccgagcc aaagcagcca ctgccggtgg cgcggcggcc tcggccctgc
     9301 ccgctcccgc gcgcgtgctc cgacacgcgc ccgccacgtt cgctgctggg cgacggtcgt
     9361 gctcatgtgt cctccgacag cgtccggatg ttgccgccca cgaaccggat gtacagcagc
     9421 gacaccgcca tcacgatgat gaacgtaagc accgcgagcg cggatccctg cccgacgagc
     9481 cggtcctgcg tgagcgtctg atacgcctga agcgagagcg tctcggtgtc gtttgcgccc
     9541 ttcgtgagca cgaacggcag gtcgaagatg cgcagcgcgt cgagcgttcg gaagatcaac
     9601 gccacgagga tcgccggctt cagcagcggc agcgtgatcc tcgtgaagcg ctgccacgcc
     9661 gtcgcgccgt ccaccttcgc ggcgtcgtag acgtcgttgg ggatcacctg aagcccggcc
     9721 aggatcagca gcgccatgaa cggcgccgtt ttccagacgt ccgcgaagat catcaccgcc
     9781 agcgcgtaac cgctctggcc gagccacacc acgtgcccgc ccggcaggcc tagcgcgtcg
     9841 agcacctggt tcgcgaagcc caggtcgggc tcgaagatcg agcgccacat gatcgccgtg
     9901 ccgaccgtca gcacggccca gggcacgagc accgtggcgc gcaggattcc ccggcccttg
     9961 aacgactcgt gcatgagcag cgccatgccc agcccgagca ccagctcgag actcaccgag
    10021 atgccggtga agatgaaggt ggtcttgaag gcgttccaga agtcagacga cttcagcgcg
    10081 tcgaggtagt tgtcgagccc cacccagcgg gagaggcccg gcacgcgcac gctgtactgg
    10141 ttcagcgaga gccagatcgc gtagccgatc ggataggccg ccaccaccgc gatcacgatc
    10201 agcgacggcg acagcatcgc cccggcgagc tggcgctcgg agagcccgcg ccgcctgtgc
    10261 ggcgcgcgta cccgacctac cgtggcctca gccgtggtca tggtgtcgct agaacgtctt
    10321 cagtgccgcg ttgatctggc tctgcatctt ctgcagtgcc gacttcgggg atgtgccctg
    10381 cagagccgag tagacgttct tgaagatcgc ctcgctgatc tgcggataga cgggcgagac
    10441 cgggcgtgcc ttgccctgct gcacgccctt cagcagctcg gccgcgaatg gcaccttctt
    10501 cttgacctcc ggatcggtgt agaccgaggt cagcaccgcc ggcagcgacg acttgatgaa
    10561 gaacttcttc tgcgccgccg gggccgtcgc gaagttgatg aactcgagcg cgccgcctgg
    10621 gttcttcgag tacgccgaga tgccgaggtt gtagccgccg atcacgctcg cgctcgcgcc
    10681 gccctgccac ttcggcagcg gcgtcacgcc gaagttcttc ttggcggcga ccgcctgcgc
    10741 atatacgtac ggccagttac gcaggagcac gtccttgtca gtgagccacg cgcgcttcga
    10801 ctcctcctcc atgtaggtga ggttcgcctt cggcgcggcg ccgtccttga tgttgttttg
    10861 catctgggtc agcacctgct cggcctgagg cgagtccact gtcgacttcg acccgtccga
    10921 gttcagcacg gtgccgccag ccgcgtagag catctcgagg aagttcaccg tcaggccctc
    10981 gtactgcgcg ccctggaacg cgaaaccgcc gtccgacttc gccatggaga acgtctgctg
    11041 ccatgtggag ggcggcgtct tgaccttgct cttgtcgtag tagatgaagc ccgcgttcgt
    11101 gttgaacggg acggcccagt acttgtcgcc gatcttcgcg gtgttgatcg tcgacgggat
    11161 gaactcgttc tttcgagcgt tgatggcagg tgtcacgttg aggagccagt tctggccggc
    11221 gaactcgccg gtccagatca cgtccatgcc gagcacgtcg cactcaggtg acttcgcgcg
    11281 caggcgctgg atcagctggt tgcgctgctc atccgccgcc gtcggaagct cggtgagctt
    11341 caccttgacg ttggggttct gctggttgtg cagcttcacg acctgactga aggcgccggt
    11401 cgtgtccttg ccgatgcacc agttcacgtt gcccttggcg ttttgcgcct ggccgggcgt
    11461 gacggttttc gcgttctcgc cgacgttgct gggggtggta ccgccgccgc ctccgccccc
    11521 gccgccgcag ccggctgcga cagccgccac gaatgcaatg gccaggccgc tcgaaagctt
    11581 gctgttcaca gaactcccct cctcgacatc cgcggcgggc ccccgcgcgg acgtcggctc
    11641 tcatcccagt tcgcctcttc gttacccgcc tcaagccggt gcgaatcacc gcaggtgtga
    11701 tgctgcggtt aaggtgtcgc gcccatgagc ctcgacgcgg tcgtgatcgg cgccggccac
    11761 aacggcctgg tcgccgccgc ctacctggct cgcgccggtc tcgacgtcga ggttctggag
    11821 cgccgcgacg tcgtcggcgg cgcctgcgtg accgaggagc tgtggcccgg ggtccg
//
LOCUS       DASVXS010000128        16114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1307089, whole genome shotgun sequence.
ACCESSION   DASVXS010000128 DASVXS010000000
VERSION     DASVXS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16114
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307089"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VF752_07035"
     CDS             complement(<1..601)
                     /locus_tag="VF752_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8855340.1"
                     /translation="MPALVPDLPQELQVEVTGACNLRCRMCLVRYAPAIGKREGALSY
                     EQFIELVNSLPRLRKLTLQGLGEPLLSPHLVAMVEHAASRGTRVGFNTNGTLLGRRRA
                     EELVASGLGWLHVSLDGATAATYEDVRHGTDFRPHAGQFERVVANLRGLVSTRREAGS
                     ADPRIQLVFVAMRRNINELEALLDLGADIGVDSVWVQNLS"
     gene            759..1433
                     /locus_tag="VF752_07040"
     CDS             759..1433
                     /locus_tag="VF752_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX8855341.1"
                     /translation="MAVYAGRPVTRGAGEETATLHAPIADPAPLGLAAFAGTTMMLSA
                     VNAGWVDAKVVPAVLGMALFYGGAAQLLAGMWEFRRNNTFAAAAFSSFGAFWLAFWAF
                     EQFYADKIPAAQAGNAVGLFLLAWAIFTTYMIIASLRVSVAVATVFVLLAATFWFLAF
                     GEFETSKNLTKVGGYVGIVTAAVAWYTAFAGVVNETWKRTILPVGHTAPPAAAIGKTE
                     PTETAG"
     gene            complement(1450..1860)
                     /locus_tag="VF752_07045"
     CDS             complement(1450..1860)
                     /locus_tag="VF752_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855342.1"
                     /translation="MRALAVTAASAAALALASCGGGGNGGGSVPESATKTTPASLAKL
                     PSGARTEVQQANAKIASYCAQAAKSLDNPQGSLPTTATDGSLHKAVSSLIAVSKLHPK
                     EMAPVLFQAQKTLQSGCYPSQGERLNAARLAIGG"
     gene            complement(1896..2846)
                     /locus_tag="VF752_07050"
     CDS             complement(1896..2846)
                     /locus_tag="VF752_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX8855343.1"
                     /translation="MTALLLLALAGNASATLVTSDVTQEALGTNPAVVNFTAPTQSSI
                     WPFAVTSTCTPASGSNFTVGTTAVTCLSVPLDTGTFNVNVIAGTGPTLVQPSNVLAYA
                     DAGKSSAVATYPLPAAGDPSGVKSLTCAPASGSRFALGNTLVRCDAKDGPGNPSSVAF
                     WVTVANHTASSEDGADGGTSGARPVVLTGGTTYVVKGGRTRVTLACPAGNPYSCTGVT
                     AFYVRLATGKSATGSGSLVNLGSATFSIPPGKSAAVTVKITRTGQRLLKRLKRFSALQ
                     QASTADPGGRELRSTRVVTLKQGKVKKPKKPQKKGPRHRH"
     gene            2952..4031
                     /locus_tag="VF752_07055"
     CDS             2952..4031
                     /locus_tag="VF752_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8855344.1"
                     /translation="MAGLALFTGFPGFIGRRLVARLLADDPKLRIVALVEQRMVDAAR
                     EAAASLDAGDRVEVLPGDIVDRRLGLSDDDYQRLQSGITRAFHLAAIYNLAVPLEMAQ
                     RVNVDGTGNVLELCASAKKLERLEHVSTAYVAGVRTGVVYEHELVMGQDFKNHYESTK
                     FQAELWVRQLMDRVPATIYRPAIVVGDSHTGETQKFDGPYYMLRSIALSAQRNMPIAQ
                     FGRSEAPFNCVPVDFIVDAMAVGITDAGITGRTLHLVDPDPVTAREVFDILAREYAGR
                     GPAYRVPPRMVETALRMPAVRDAFGGAPGEGIRYLNHPVRFDTRNADDFLARHGLHVP
                     SIHEYAPAMVRFFREHEHDEAYVPA"
     gene            4250..4615
                     /locus_tag="VF752_07060"
     CDS             4250..4615
                     /locus_tag="VF752_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855345.1"
                     /translation="MADRVLFISWGESVAGREERGLEVFNEALGLYGRLQQDGRIESF
                     NVAILEPNGGVEGYMEIYGSADQLAALRQSDEFRRTMTDASLIVHDLRMVEGSVNEGV
                     ARDVALYQESIAKVPQATA"
     gene            4738..4962
                     /locus_tag="VF752_07065"
     CDS             4738..4962
                     /locus_tag="VF752_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855346.1"
                     /translation="MDENSRLIALIRSLSERSLAESDALATTLGRRCWPGGGDRADPH
                     AAEWVRRWHPRSMPVPVHCSCAAGRCLVCN"
     gene            5039..5269
                     /locus_tag="VF752_07070"
     CDS             5039..5269
                     /locus_tag="VF752_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855347.1"
                     /translation="MDDRLRVTALIDWVANRSDAETEDLAWALRRRCWPGGVTDRNEP
                     GSHDWIRKSPPSNAAQLLLHCECERRRCHVCN"
     gene            complement(5456..6724)
                     /locus_tag="VF752_07075"
     CDS             complement(5456..6724)
                     /locus_tag="VF752_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX8855348.1"
                     /translation="MAQALPMTQRISYTDLYERWEKSNWSATALDFSVDKEHWQERFS
                     DKQRRAALWNYALFLHGEDSVADNLSPYIEAAPKEEMKYFLATQQVDEARHAVFFARF
                     MREVVEDGADDVAGSLARTRPDLTWGFRQVFERLDKMADELRRDRSLPMLAKGIALYH
                     IVIEATLAQPGQHFIQSYLERDELMPGFQEGMRNVALDEQRHIGFGVKVLADLVQMDP
                     ECRDAVAELLREVMPKSVGVLVPPGWDRSYTECFGFTLEDIYTEAILSMETKMRSAGL
                     PLEELPGALPIDLSVPPRERAERAIKLLEGNIIGEKLGPPARDPETQALMFDSVRLSV
                     DPAAANGRTTIQWDFTDSEPWVLHVDNGSTRVEQAWAEDPDITLRARYEDWVDISAGR
                     TDPWLAIATGKLRPRGKLRSILKMAKLFPR"
     gene            6776..7375
                     /locus_tag="VF752_07080"
     CDS             6776..7375
                     /locus_tag="VF752_07080"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8855349.1"
                     /translation="MPLAALINVPANLGYLALAGIVGFESMGVPLPGETALIAAALLA
                     KRGDLSIALVVPIAAAAAIVGDNAGYVIGRKGGRRLLLRDGPLLEQRTRLLDRGEAFF
                     SRHGAKAVFLGRWFAGLRITAAWLAGIHHMPWRSFLFWNALGGIAWAVTVGVLVYIAG
                     HVIEDVVKTGGIAGAAVIVLAGGAFLMWRWMRGRSEARR"
     gene            7387..8166
                     /locus_tag="VF752_07085"
     CDS             7387..8166
                     /locus_tag="VF752_07085"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX8855350.1"
                     /translation="MADTPLSGRRAIVTGASSGLGKETVRRLAADGAAVAVNYIGNAD
                     AAEEIVEKIRSDGGTAIAVQADVSKESDVVGMFEEIREGLGGCVNVLVNNAGIEKPFK
                     LVDMPLDEWNKVLSVNLTGAFLCAREAARQMIAADEGGVIVNISSVHELIPWPEFSHY
                     CASKGGMRMWSKTIARELAPHQIRVVNIGPGAMLTPINQDLIDHPEKRKEIEEEVPLG
                     RLGEPSEVASAVAWLVSEQASYITGTTLFVDGGMTLYPKFV"
     gene            8233..9087
                     /locus_tag="VF752_07090"
     CDS             8233..9087
                     /locus_tag="VF752_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX8855351.1"
                     /translation="MTRAGTSALTLSEEIRQRFDEFSRSQKDVGQYIVDHLDEAAFQT
                     AEELARRANTSSSTVVRFAQALGFEGFPELQQAARDEYRRSREGNAGQVESLLAPPLF
                     PIDQTEFEAALAADHTNVEDTARKVDRDEVSAAIDLISRAERIVLCGTDQMAFFASYL
                     RHLLMLLDLRCEVVASPSQEGLARLGRIDDKTLVIGFSAGRPHPLVVRAVKLARHRKA
                     RTIAVADATLSEVAKLADHKLYYSSNSPAYVRSHAALLSLVQALAYGVYALDESAYAD
                     RIKAFRLK"
     gene            9110..9661
                     /locus_tag="VF752_07095"
     CDS             9110..9661
                     /locus_tag="VF752_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855352.1"
                     /translation="MPAPLTAHGLRRAKKLEPVAATAREREDLAEQPYGLEDLGAFPV
                     AAEEPGTYPHEIEGERRAGRKGRIRLVLATIAFPVVLAALFSKTWQLMPFPDTEAALA
                     ALGTGFAFGLAVHRWSAMWLTLAVLPAGMGGEGGFFGGVVALFVVGPFALVGLTAGVA
                     LVKRLARVAIRRMIAPARTPLSA"
     gene            9867..10760
                     /gene="pdxS"
                     /locus_tag="VF752_07100"
     CDS             9867..10760
                     /gene="pdxS"
                     /locus_tag="VF752_07100"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX8855353.1"
                     /translation="MRSVKDAATFRVKSGLAEMLKGGVIMDVVTAEQARIAEAAGAAA
                     VMALERVPADIRAHGGVARMSDPTKIEEIQGAVTIPVMAKARTGHFVEAQVLEALEVD
                     YIDESEVLTPADESNHIDKWRFKVPFVCGATNLGEALRRIAEGAAMIRTKGEAGTGNV
                     VEAVRHMRTIQGEMRRLSSMDEAELPTAAKELQAPLDLVRQVAQDGKLPVVNFSAGGI
                     ATPADAALMMQLRAEGVFVGSGIFKSEDPERTAAAIVDATTHFEDAARVASASRGLGA
                     PMAGIEISELGEGGLIQHRGW"
     gene            10769..11335
                     /gene="pdxT"
                     /locus_tag="VF752_07105"
     CDS             10769..11335
                     /gene="pdxT"
                     /locus_tag="VF752_07105"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX8855354.1"
                     /translation="MRIGVLAIQGGFEAHARVLERLGAEPVEVRTPAGLAGLDGLIIP
                     GGESTTMTLGIEREGLADPLREYVRSGAPVLGTCAGLIMLDRDHLGLLDVAAERNAFG
                     RQVHSFEADLTVAGFDGPLRAVFIRAPWVGEHGADVEVLSEVDGHPVAVRQGNITAVA
                     FHPELTDDTRVHELLLDQVRSGRVEERV"
     gene            11332..12438
                     /locus_tag="VF752_07110"
     CDS             11332..12438
                     /locus_tag="VF752_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX8855355.1"
                     /translation="MRDPRVDALAQILVRYSTKVQKGEIVSIQGPTTGEPLLQAIYEE
                     VLRSGGLPIMQIGLEEQAPAFYELASDEQLEWIPPTAKWTIENCDVRFAVMADTNPRQ
                     LQSVDPKKQALVQRSRKEIMETSMRRSAAGEYRWNVTLFPTQAYAAEAGLSLGSYEDF
                     FYSACLATDPEPLTAWQRQSDQVNRLTEWITGKEEVHIKAPGTDITLGVAGRTWIPCV
                     GLHNMPDGEFFTGPVEDSVEGEISFSFPATYGGREVSGVRLKFDGGKVVDASAEQGEA
                     FLIEMLDTDKGARRLGELGIGTNYGISTGTKEILLDEKIGGTIHMAVGMSYPESGGKN
                     ESAVHWDMVCDLRSGGAITVDGTELQRDGRFLVV"
     gene            12517..13071
                     /locus_tag="VF752_07115"
     CDS             12517..13071
                     /locus_tag="VF752_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855356.1"
                     /translation="MPRYRLLGLAAAVAVFAAALIGAWVVFTPSGSGERVLEKHRVYF
                     AYQNTPLRASVETRWNRERASGALASPDGLGLRSRCVEASMFSYLSRNWREGPVKAAA
                     MILTGTFAEQSLYNVRAVTRTATSALVVDPPLSAITLVRRSGTRDYTALVAQFTQQPG
                     CSSSEVSSGLKELRRVVDSFRVGA"
     gene            13117..13917
                     /locus_tag="VF752_07120"
     CDS             13117..13917
                     /locus_tag="VF752_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855357.1"
                     /translation="MTRDWLAVPRDWMESFGDIARFCARVIYEVFTLRVMRFFGEALR
                     QAGILILGSTLVIASLAFILGLQCGIEGAYFTRAQGAPSYAGVFAAWCGLRELLPYAF
                     GYMMAAKVGTGLVAEIGAMRISDEIDALEVMGINSITFLCATRLLASFMVLPFMYLTA
                     LGAGYLASYIAVVKQIGEVSSGGYLLIFWMFQNPPDVLYSLVKGMAMAVVVVLVGCYY
                     GYNASGGPVGVGTATAKSMVLNTVAVHLVGMMGTQLFWGAKPRAPIGG"
     gene            13923..14291
                     /locus_tag="VF752_07125"
     CDS             13923..14291
                     /locus_tag="VF752_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855358.1"
                     /translation="MRRRTPPPHAPWGAFPLSELCVLLSLMCAAASLVLWGTARGLWL
                     VAAAGVLGCLAGCEVAYREHFAGYRSHTAPLAGSVAIPLAVGLVFAGMPAIVVLVSGG
                     AAFLAAVPLFERAFRRRAGL"
     gene            complement(14288..15085)
                     /locus_tag="VF752_07130"
     CDS             complement(14288..15085)
                     /locus_tag="VF752_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX8855359.1"
                     /translation="MPATQEEKARAFRALHEGEPFVIPNPWDAGSARVLEALGFKALA
                     TTSSGFAFTLGRLDGGVTLEQVVRHISVLASATNVPISADLENGYGRSPEEAATAIRS
                     AAEAGAVGGSIEDWDPSGELYPIEEATERVAAAVEAARALDFPFTLTARAENHIRGNP
                     DLDDTIARLQSYERAGADVLYAPGLRSGDEIRAVSEATSKPLNVLAHRGLTMPEIVDA
                     GGQRVSVGGALAWQALGGLVTAAERIRDEGDFSVLDQRGPRPEWLGG"
     gene            complement(15102..>16114)
                     /locus_tag="VF752_07135"
     CDS             complement(15102..>16114)
                     /locus_tag="VF752_07135"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855360.1"
                     /translation="LCPRAVVVPPRMNAYTEASRAMFRVFADTTPLVEGLSIDEAFLD
                     VRGLEHISGTPAEIAVKLRRAVRERVGIPITVGVARTKFLAKVASGVAKPDGMLVVPP
                     AGELAFLHPLPVERLWGVGAVTSTKLRERGLETVGEVARLSEADLVEMLGKASGRHLH
                     ALAHNRDPRRVERRKRRRSMGAQHALGRRAPKSPEALDAVLVGLVDKLTRRLRAARRV
                     CRTVVLRLRFDDFSRVTRSHTLLQATSETETVLATGRELLAAALPMIEDRGITLLGIA
                     LGNLSDGGAVQLALPFERERAWALDTTLDELRDRFGAGAVTRAVLLGRDHGPSVPLLP
                     DD"
BASE COUNT         2388 a         5559 c         5612 g         2555 t
ORIGIN      
        1 ggctcaggtt ctgcacccac acagagtcga cgccgatgtc cgcgcccagg tccaaaagcg
       61 cctcgagctc gttgatgttg cgccgcatgg ccacgaacac cagctggatg cgcggatcgg
      121 cggagcccgc ctcgcggcgc gtcgacacca gcccgcgcag gttcgccacg acgcgctcga
      181 actggccggc gtgcggccgg aagtccgtcc catggcggac gtcctcgtag gtggccgcgg
      241 tagcgccgtc gagtgacacg tgcagccagc cgaggcccga cgcgacgagc tcctccgcgc
      301 gccggcggcc gagcagcgtt ccgttcgtgt tgaagcccac ccgggtacca cgcgaggccg
      361 catgctcgac catcgccacc aggtgcggtg agagcagcgg ctccccgagc ccctgaagcg
      421 tgagcttccg caggcgcggg agcgagttga ccagctcgat gaactgctcg tagctgagcg
      481 cgccctcccg cttgccaatc gccggcgcgt agcgcaccag gcacatgcgg cagcgcaggt
      541 tgcacgcgcc ggtgacctcc acttggagct cctgggggag gtctgggacg agtgccggca
      601 tgtgcggcgg ttacccgagc atgccgcagg gaaacggcgc gcccagacgg ggtaatggag
      661 gctggtaatg acgcgtttcg gcgcgtgtga ccgggtactc ctcaagatgt gggaggacgg
      721 gtgtaccgcc ctcgcttcaa cggaggaggg aatgcacaat ggccgtttac gcaggccgcc
      781 ccgttacacg cggtgcgggc gaggaaaccg ccacgttaca cgcgccgata gctgatccgg
      841 cgccgctggg actcgcggca tttgcgggca ccacgatgat gctgtccgcg gtcaacgcgg
      901 gctgggtcga cgctaaggtc gtgcccgcag tgctgggcat ggcgctcttc tacggagggg
      961 ccgcgcagct gcttgcgggc atgtgggagt tccgccgcaa caacacgttc gcggctgcgg
     1021 cgttcagctc gttcggagcc ttctggctcg cgttctgggc gttcgagcag ttttacgcgg
     1081 acaagattcc ggccgcccag gccggtaatg cggtgggcct gttcctgctc gcatgggcga
     1141 tcttcaccac gtacatgatc atcgcctcgc tgagggtctc cgtcgcagtt gcgacggtgt
     1201 tcgtcctgct tgccgccacg ttctggttcc tggcgttcgg tgagttcgag acgagcaaga
     1261 acctcaccaa ggtcggcggg tacgtcggca tcgtcacagc ggcggtcgcc tggtataccg
     1321 cattcgccgg ggtcgtgaac gagacgtgga agcgcacgat cctgccggtc gggcacaccg
     1381 ctccgccggc agcggcgatc ggcaagacgg agccaacaga gacagccgga taacgcgaca
     1441 gggcgccgct cagccgccga tggcgagccg ggcggcgttc agccgctcgc cctgggacgg
     1501 gtagcagccc gactgcaggg tcttctgcgc ctggaacagg accggcgcca tctccttcgg
     1561 gtgcagcttg ctgaccgcga tcagcgagct caccgccttg tgcagcgagc cgtcggtggc
     1621 ggtcgtcgga agcgaaccct gcgggttgtc gagcgacttt gccgcctgtg cgcagtaaga
     1681 cgcgatcttc gcgttcgcct gctgcacctc ggttcgcgcc ccggacggta gtttcgcgag
     1741 cgaggccggc gtggtcttcg tggccgactc cggcacggaa ccaccgccgt tgcctccacc
     1801 cccgcatgag gcgagcgcca gcgcggcagc cgaggccgct gtgacagcaa gcgcgcgcat
     1861 gcggaaatcg taggccgtgg acgccccgtt gcgcgctagt gccggtggcg agggcccttc
     1921 ttctgcggct tcttaggttt cttcactttc ccctgcttca gcgtcaccac gcgggtcgag
     1981 cgaagctccc tgcccccggg gtcggcggtg ctggcctgct ggagcgccga gaagcgcttc
     2041 agtcgcttga gaagccgctg gccggtgcgg gtgatcttga ccgtgacggc cgcggacttg
     2101 ccgggcggga tcgagaacgt tgcgctgccg aggttcacga gactgcctga gccggtggcc
     2161 gacttcccgg tcgcgaggcg gacgtagaac gccgtcaccc cggtgcacga gtacgggttg
     2221 cctgccgggc aggcgagcgt gacgcgcgtg cggccgccct tcaccacgta tgtcgtgccg
     2281 cccgtcagca ccaccggacg ggcgccgctc gttccgccgt cggcgccgtc ctccgaggac
     2341 gcggtgtggt tggcgacggt cacccagaag gcgacgctcg aggggttgcc cgggccgtcc
     2401 ttcgcgtcgc agcgcacgag cgtgttgccg agcgcgaacc tgctccccga cgcgggcgcg
     2461 cacgtgagcg acttcacgcc ggacgggtcg cccgccgcgg ggagcggata cgtggcgacc
     2521 gccgacgact tcccggcatc cgcgtaggcg agcacgttcg acggctgtac cagcgtcgga
     2581 ccggtgccgg cgatcacgtt gacgttgaag gtgccggtgt cgagcggcac cgataggcat
     2641 gtgaccgcgg ttgtccccac ggtgaagttc gacccggatg ccggcgtgca ggtcgaggta
     2701 acggcgaatg gccaaatact gctctgcgta ggcgccgtga agttcacgac cgcggggttc
     2761 gtgccgagcg cctcctgcgt cacgtcgctc gtgacgaggg tcgccgacgc gttgcccgcg
     2821 agggcaagca acagcagcgc cgtcaccgcc gcgatcctca tcccggtcgc gcaagttacg
     2881 ccacctggcg cgtttgcgcg ccaattctcg acaaagcgac gatgtagcga gcgaactaga
     2941 ctgcccgcct cgtggcaggg ctggcgctat tcacaggctt cccgggcttc atcggcaggc
     3001 gcctcgtggc gcgtctgctg gccgacgacc cgaaacttcg gatcgtcgcg ctcgtggagc
     3061 agcggatggt cgatgcggcg cgcgaggccg ccgcctcgct cgacgcaggg gacagggtgg
     3121 aggtgctccc gggcgacatc gtcgaccgcc gcctcggtct ttccgacgac gactaccagc
     3181 gactgcagtc cggcatcaca cgcgcgttcc accttgccgc gatctacaac ctcgcagtgc
     3241 cgctcgagat ggcccagcgc gtgaacgtgg acggcacggg caacgtgctc gagctctgcg
     3301 cgagcgcgaa gaagttggag cggctcgagc atgtgagcac ggcctacgtc gccggggtgc
     3361 ggaccggcgt ggtgtacgag cacgagctcg tgatgggcca ggacttcaag aaccactacg
     3421 agtcgacgaa gttccaggcg gagttgtggg tgcgccagct gatggaccgc gtgccggcga
     3481 cgatctatcg cccggcgatc gttgtgggtg actcacacac gggcgagacg cagaagttcg
     3541 atgggccgta ctacatgctc cgctcgatcg cgctatcggc ccagcgcaac atgccgatcg
     3601 cgcagttcgg ccgctcggag gcgccgttca actgcgtgcc cgtggacttc atcgtcgacg
     3661 cgatggccgt cggcatcacg gacgccggga tcaccgggcg cacgctgcac ctggtggatc
     3721 ccgatcccgt taccgcgcgc gaggtgttcg acatcctcgc gcgcgagtac gcggggaggg
     3781 gcccggccta ccgagtgccg ccgcgaatgg tcgagacggc tctgcgcatg cccgccgtcc
     3841 gcgatgcctt cggcggcgcg ccgggcgagg gcatccgcta cctcaaccat cccgtgcgct
     3901 tcgacacccg caacgcagac gacttccttg cgcggcacgg acttcacgtc ccgagcatcc
     3961 acgagtacgc gcccgcgatg gttcgcttct tccgcgagca cgagcacgac gaggcatacg
     4021 tcccggcgta gcgcgccaac cccgcgcgct aagtcctgac tggcgccaaa cgtcgtcact
     4081 ttggggcgta tatcgaccct aagtgacgac actcggtccg ggccacgccg ggccacccct
     4141 ccatactgct gttcgagccg cgtgacaacc gcttgcgccg ctgcgtcgcc gctgagagag
     4201 tcgtacgaaa ggcccggcaa agggccggac cgcgacggaa ggagtcggaa tggccgatcg
     4261 agtgctcttc atctcgtggg gcgaatccgt agccgggcgc gaggagcgcg gcctcgaggt
     4321 gttcaacgag gccctagggc tgtacgggcg actccagcag gacggccgca tcgagagctt
     4381 caacgtggcg atactcgagc cgaacggcgg cgtcgagggc tacatggaga tctacgggag
     4441 cgccgaccag ctcgccgccc tgcgtcagag cgacgagttc cgacgcacga tgaccgacgc
     4501 gtcgctcatc gtccacgacc tgcggatggt tgagggctcg gtgaacgagg gggtcgcccg
     4561 cgacgtcgcc ctctaccagg agtcgatcgc gaaggtgccg caggcgacgg cctgaccccg
     4621 ccgccgtacc gtccggcgcc tcgacgggag ggcgaggcgc cgcggtcgcg gcttggattc
     4681 tcggtccggt gggtatcttt gtccggccgg aatgcgattc tgcgcgccgg ctccccgatg
     4741 gacgagaact cccgcctcat tgccctgatc aggtcgcttt ccgagcgctc actcgccgag
     4801 tccgacgcgc tcgcgaccac gcttgggcgc cgctgctggc ccgggggcgg cgatcgcgcc
     4861 gacccacacg cggccgagtg ggtacgccgc tggcacccgc gctcgatgcc cgtgcctgtc
     4921 cactgctcgt gcgccgcggg ccgctgcctc gtgtgtaact gactgaggac gttccggact
     4981 ttatgaacac gtttccggct tcactcggcg ccagggcggg tatttcttct cgctgacgat
     5041 ggacgatcga ctacgagtaa cggcgctgat cgactgggtc gccaaccgca gcgacgcgga
     5101 gacggaggac ctggcctggg cgctcaggcg ccgctgctgg ccgggcggcg tgaccgaccg
     5161 caacgagccc ggctcgcacg actggatccg caagtcgccg ccgtcgaacg ccgcccagct
     5221 gctgctccac tgcgagtgcg agcggcggcg ctgccacgtc tgtaactagc tgcccgcgcc
     5281 cggcgcggct gttgcgccga cgcaaacagc agcaagaggg gtggtcgagg cgaagtgcgg
     5341 aacctttggg cgcctttagc cgctcaaatg ttccgcagat cggcccggag ccggtggagc
     5401 ccttccctca gtgctcaaaa cgctggcggc gcgcagccgc cggcggcaga cgccctcagc
     5461 gcgggaacag cttcgccatc ttcagaatcg agcgcagctt gccccgcggg cgcaacttgc
     5521 ccgtggcgat cgcgagccac gggtccgtgc ggcccgcgct gatgtcgacc cagtcctcgt
     5581 aacgcgcgcg gagcgtgatg tccgggtcct ccgcccacgc ctgctccacg cgcgtcgagc
     5641 cgttgtccac gtgcagcacc cacggctcgg aatcggtgaa atcccactgg atcgtggtcc
     5701 gcccgttcgc cgccgccggg tccaccgaca gccgtaccga gtcgaacatc agcgcctgcg
     5761 tctccgggtc gcgcgccggc gggccgagct tctcgccgat gatgtttccc tcgagcagtt
     5821 tgatcgcgcg ctccgcgcgc tcgcgcggcg gcacgctcag gtcgatcgga agcgcccccg
     5881 gtagctcctc gagcggcagc ccggccgagc gcatcttcgt ctccatcgag aggatcgcct
     5941 cggtgtagat gtcctcgagc gtgaagccga agcactcggt gtagctgcgg tcccagcccg
     6001 gcggcacgag cacccccacc gacttcggca tcacctcgcg cagcagctcg gcgacagcgt
     6061 ccctgcactc cgggtccatc tgcacgaggt cggcgagcac cttcacgccg aagccgatgt
     6121 gccgctgctc gtcgagcgcg acgttgcgca tgccctcctg gaagccaggc atcagctcgt
     6181 cgcgctcgag gtaggactgg atgaagtgct gcccgggctg ggcgagggtc gcctcgatca
     6241 cgatgtggta cagcgcgatg cccttcgcga gcatcggcag cgagcgatcg cggcggagct
     6301 cgtcggccat cttgtcgagc cgctcgaaca cttgacggaa gccccacgtg aggtccggcc
     6361 gcgtccgcgc gagcgagccg gccacgtcgt ccgcgccgtc ctccacgacc tcgcgcatga
     6421 agcgcgcgaa gaacacggcg tggcgcgcct cgtcgacctg ctgggtcgcg aggaagtact
     6481 tcatctcctc cttcggcgcg gcctcgatgt agggggagag gttgtccgcg acggagtcct
     6541 cgccgtgcag gaacagcgcg tagttccaga gagcggcgcg gcgctgcttg tcgctgaaac
     6601 gctcctgcca gtgctccttg tcgacggaga agtcgagagc ggtggcgctc cagttgctct
     6661 tctcccagcg ctcgtagaga tccgtgtacg agatccgctg ggtcatcggg agcgcctgtg
     6721 ccatgttgga acctctcttc gccgtttggc tgaccagcca aaatgattcc agacgatgcc
     6781 tctggcggcc ctgatcaacg tccccgcgaa cctgggttac ctcgcgctcg ccggcatcgt
     6841 cggcttcgag tcgatgggcg tgccgctgcc gggcgagacg gcgctcatcg cggcggcgct
     6901 gctcgccaaa cggggcgacc tgtcgatcgc gcttgtggtc ccgattgccg cggccgcggc
     6961 gatcgtcggc gacaacgccg gctacgtgat cggccgcaag ggcggccgcc ggctgctcct
     7021 gcgcgacggc ccgctgctcg agcagcgcac ccgcctgctc gaccgcggtg aggccttctt
     7081 ctcgcgtcac ggcgcgaagg cggtgttcct cgggcgctgg ttcgccggcc tgcgcatcac
     7141 cgccgcgtgg ctcgccggca tccaccacat gccgtggcgc tcgttcctgt tctggaacgc
     7201 gctcggcgga atcgcctggg cggtcaccgt gggtgtgctt gtctacatcg ccgggcacgt
     7261 gatcgaggac gtcgtgaaga ccggcggcat cgcgggcgcg gccgtgatcg tgctcgcggg
     7321 tggcgccttc ctcatgtggc gctggatgcg tgggcgcagc gaagcgcggc ggtagccttg
     7381 tccgccatgg cggacacgcc actgagcggg aggcgggcga tcgtgaccgg agccagctcc
     7441 ggcctcggca aggagacggt tcggcggctc gccgccgacg gtgcggcggt agcggtcaac
     7501 tacatcggca atgctgacgc cgccgaggag atcgtcgaga agatccgctc ggacggcggc
     7561 accgcgatcg ccgtgcaggc cgacgtgtcc aaagagtccg acgtggtcgg gatgttcgag
     7621 gagattcgcg aggggctcgg cggctgcgtg aacgtgctcg tgaacaacgc aggcatcgag
     7681 aagccgttca agctcgtgga catgccgctc gacgaatgga acaaggtgct ctcggtgaac
     7741 ctcacgggcg ccttcctctg cgcgcgcgag gcggcgcgcc agatgatcgc cgccgatgag
     7801 ggcggcgtga tcgtgaacat ctcgagcgtg cacgagctga tcccgtggcc cgagttcagc
     7861 cactactgcg cgagcaaggg cggcatgcgg atgtggtcca agaccatcgc tagggagctc
     7921 gcaccgcatc agatccgcgt cgtgaacatc gggcccggcg cgatgctcac cccgatcaac
     7981 caggacctga tcgatcaccc cgagaagcgc aaggagatcg aggaggaggt cccgctcggg
     8041 cgactcggcg agccgtccga ggtcgcctcc gcagtcgcct ggttggtgag cgaacaggcg
     8101 agctacatca ccggcacgac gctcttcgtc gacggtggaa tgactctcta tccgaagttc
     8161 gtctgaccgt cccggcccgc ccggacccgc gcgcggaagg aacttcttcc cgacgcgcga
     8221 atcccttgga ccgtgacccg agcaggcacc agtgccctga ccctttcgga ggagatccgt
     8281 cagcgcttcg acgagttctc ccgctctcag aaggacgtgg gccagtacat cgtcgaccac
     8341 ctcgacgagg cggccttcca gacggccgag gagcttgccc gccgcgccaa cacctcgagc
     8401 tccaccgtcg tccgcttcgc gcaggcgctc ggcttcgagg gcttccccga gctccagcag
     8461 gccgcgcgcg acgagtaccg ccgctcccgc gaaggcaacg cggggcaggt cgagagcctc
     8521 ctcgcgccgc cgctcttccc aatcgaccag accgagttcg aggccgcgct ggcggcggac
     8581 cacacgaacg tcgaggacac cgcgcgcaag gtggaccgcg acgaggtctc ggccgcgatc
     8641 gacctgatct cgcgcgcgga gcgcatcgtg ctatgcggca ccgaccagat ggcgttcttc
     8701 gcgagctacc tgcggcacct gctgatgctg ctcgatctgc gctgtgaggt cgtcgcgagt
     8761 ccgagccagg aggggctcgc gcgcctcggc cggatcgacg acaagacgct cgtgatcggc
     8821 ttctccgccg gacgcccaca cccgctggtc gtccgcgcgg tgaagctcgc ccgccaccgc
     8881 aaggcgcgca cgatcgcggt cgccgacgcg actctgtccg aggtcgcgaa gctcgccgac
     8941 cacaagctct actactcctc caacagcccg gcgtacgtgc gctcgcacgc cgccctgctg
     9001 agcctcgtcc aggcgctcgc ctacggcgtc tacgcgctcg atgagtcggc ctacgcggat
     9061 cgcatcaagg cgttccgcct caagtagcga tcacgaggag ctcaaaccct tgcccgcgcc
     9121 gctcaccgct catgggctca ggcgggccaa gaagctcgag cccgtagccg caaccgcacg
     9181 cgagcgtgag gacctcgccg agcagcccta tggcctcgaa gacctcggcg cattcccggt
     9241 cgctgcagaa gagcctggca cgtacccgca cgaaatcgag ggtgagcggc gcgccggacg
     9301 gaaggggcgg atccgtctgg tcctcgccac gatcgcgttc ccggtggtgc tcgccgccct
     9361 gttctcgaag acctggcagc tgatgccgtt cccagacacc gaggccgcgc tcgcagcgct
     9421 gggcaccggg ttcgccttcg ggctcgcagt gcaccgctgg tcggcgatgt ggctcacgct
     9481 ggcagtgctc ccggccggca tgggcggcga gggcggcttc ttcggcggtg tagtcgcgct
     9541 cttcgtcgtg ggcccgttcg cgctcgtggg cctgaccgcg ggtgtggcgc tcgtgaagcg
     9601 cctcgccagg gtggcaatcc ggcgcatgat cgcgccggcc cgcactcctc tctccgcctg
     9661 agagaagagt gccgggaggc tccacgctcc tcctggcctt ggagcgaaga aagtccttgg
     9721 actcgaggac tcacctcccg gcacactcca cggaagcacc ttctgaccaa tcggtcaagt
     9781 gacagcagtc actcgcctca tcgcacattg cggcatgact acgttgtcct ccgcgtcact
     9841 ctggggaatg cgcctgcatg gctaagatga ggtccgtgaa ggatgcagcc actttccggg
     9901 tgaaatccgg ccttgcggag atgctgaagg gcggcgtgat catggacgtg gtcaccgccg
     9961 agcaggccag aatcgccgag gccgcgggcg ccgccgccgt catggcgctc gagcgcgtgc
    10021 cggccgacat ccgggcgcac gggggcgtcg cgcggatgtc cgatcccacg aagatcgagg
    10081 agatccaggg cgccgtcacg atccccgtga tggccaaggc gcggaccggc cacttcgtcg
    10141 aggcccaggt gctcgaggcg ctcgaggtcg attacatcga cgagtctgag gtgctcacgc
    10201 ccgccgacga gagcaaccac atcgacaagt ggcgcttcaa ggtgccgttc gtgtgcggcg
    10261 ccaccaacct cggggaggcg ctccgtcgca tcgcagaggg cgccgcgatg atccgcacca
    10321 agggcgaggc cggcaccggc aacgtggtcg aggccgtgcg ccacatgcga acgatccagg
    10381 gggagatgcg ccggctctcg tcgatggacg aggccgagct gccgaccgcc gccaaggagc
    10441 tgcaggcgcc gctcgacctc gtgcgccagg tggcccagga cgggaagctc cccgtcgtga
    10501 acttctccgc gggcgggatc gccacgccgg ccgacgccgc gctgatgatg cagctgcgcg
    10561 ccgagggcgt gttcgtgggc tccggcatct tcaagtccga ggatcccgag cgcacggccg
    10621 ccgcgatagt cgacgccacc acgcacttcg aggacgccgc gcgggtcgct tccgcgtccc
    10681 gcggcctcgg ggcgccgatg gccgggatcg agatctcgga gctcggcgag ggcggtctca
    10741 tccagcatcg cggctggtag gtagggcctt gcgtattggc gtactcgcca tccagggtgg
    10801 cttcgaggcg cacgcgcgcg tgctcgagcg gctcggagcc gagccggtcg aggtgcgcac
    10861 gcccgcaggg ctcgcgggcc tcgacggcct gatcatcccg ggcggcgagt cgaccacgat
    10921 gacgctcggc atcgagcgcg aggggctcgc ggacccgctg cgcgagtacg tgcgctcggg
    10981 cgcgccggtg ctcggtacct gcgcggggct gatcatgctc gaccgcgacc acctcgggct
    11041 tctcgacgtc gccgcggagc gcaacgcgtt cggccgccag gtgcacagct tcgaggccga
    11101 cctcaccgtc gctggcttcg acggcccgct gcgcgccgtt ttcatccgcg ctccctgggt
    11161 gggcgagcac ggggcggacg tggaggtgct cagcgaggtg gacggccacc cggtcgcggt
    11221 ccgccagggc aacatcacgg ccgttgcgtt ccacccggag ctcaccgacg acacgcgcgt
    11281 gcacgagctg ctgctggatc aggtgcgctc tgggcgggta gaagagcgcg tatgagagac
    11341 ccgcgcgtcg acgcgcttgc gcagatcctc gtccgctact cgacgaaggt gcagaagggc
    11401 gagatcgtct cgatccaggg gccgaccacc ggcgagccgc tgcttcaggc gatctacgag
    11461 gaggtgctgc gctccggcgg gctgccgatc atgcagatcg ggctcgagga gcaggcgccc
    11521 gccttctacg agctcgcctc cgacgagcag ctcgagtgga tcccgccgac cgcgaagtgg
    11581 acgatcgaga actgcgacgt gcgcttcgcc gtgatggcgg acacgaatcc gcgacagctc
    11641 cagagcgtcg acccgaagaa gcaggcgctt gtgcagcgct cgcgcaagga gatcatggag
    11701 acgtcgatgc ggcgctccgc ggcgggcgag taccgctgga atgtgacgct gtttcccacc
    11761 caggcgtacg cggcggaggc cggcctgtcg cttgggagct acgaggactt cttctattcc
    11821 gcctgcctcg cgaccgaccc cgagccgctc accgcctggc agcgccagtc cgaccaggtg
    11881 aaccggctca cggagtggat cacgggcaag gaggaggtcc acatcaaggc gcccggaacc
    11941 gacatcacgc tcggagtggc agggcgcacg tggatcccct gcgtgggact ccacaacatg
    12001 cccgacggcg aattcttcac cggcccggtc gaggacagcg tggagggcga gatctcgttc
    12061 tcgttcccgg ccacctacgg cggccgcgag gtctcaggcg tgcgcctgaa gttcgacggc
    12121 ggcaaggtgg tcgacgcttc ggcggagcag ggcgaggcgt tcctgatcga gatgctcgac
    12181 accgacaagg gcgcgcgccg cctcggcgag ctcgggatcg gcacgaacta cggcatctcg
    12241 acgggcacca aggagatcct gctcgacgag aagatcggcg gcacgatcca catggctgtg
    12301 ggaatgagct accccgagag cggcggcaag aacgagtccg ccgtgcactg ggacatggtc
    12361 tgcgacctcc gaagcggtgg cgcgatcacc gtcgacggca ccgagctcca gcgcgacggc
    12421 cgctttctcg tcgtctaagc actagtcccg cttcgtacgc aggtataggg tcggtccgga
    12481 aagaaccggc ccctcggact ggtcggacgg cagtagatgc cccggtatcg cctcctcgga
    12541 ctcgcggccg cagttgcggt gtttgctgcg gcgctgatcg gtgcctgggt ggtgttcacc
    12601 ccgtcgggct caggcgaacg ggttctcgag aagcaccgcg tctacttcgc gtaccagaac
    12661 acgccgcttc gggcgtcggt cgaaacgcgc tggaatcgcg agcgcgcatc cggcgcgctg
    12721 gccagtccgg acgggctcgg cctgcgctcg cgctgcgtgg aggcgtcgat gttctcctac
    12781 ctctcgcgga actggcggga ggggccggtg aaggccgccg cgatgatcct caccggcacg
    12841 ttcgccgagc agtcgctcta caacgtccgg gcggtcacga ggacggcgac ttccgcgctc
    12901 gtggtggatc cgccgctgtc ggccatcacg ctcgtgcgcc gctccggcac gcgtgactac
    12961 acggcccttg tggcgcagtt cactcagcag cccgggtgct cgtcgagcga ggtctcgagc
    13021 ggactgaagg agctgcgccg cgtggtcgac agcttccgcg tcggcgcctg accgcactgg
    13081 cgcgcgagcc ggcgcttcgg ttaggctcgc ccgccggtga ctcgcgactg gctggcggtg
    13141 ccgcgcgact ggatggagtc gttcggcgac atcgcgcgct tctgcgcgcg cgtgatctac
    13201 gaggtcttca cgctgcgcgt gatgcgcttc ttcggcgagg cgctgcgcca ggcgggcatc
    13261 ctcatcctcg gctcgaccct tgtaatcgcg agcctggcgt tcatcctcgg gcttcagtgc
    13321 ggcatcgagg gcgcgtactt cacgcgcgcg cagggcgccc cctcgtatgc gggcgtgttc
    13381 gcggcttggt gcggcctgcg cgagcttctg ccgtacgcgt tcggctacat gatggccgcg
    13441 aaggtcggca ccgggctcgt cgcggagatc ggtgcgatgc gcatctccga cgagatcgac
    13501 gcgctcgagg tgatgggcat caactcgatc acgttcctct gcgctacccg cctgctcgcc
    13561 tcgttcatgg tgctgccgtt catgtacctc accgcgctcg gcgcgggcta cctcgcttcg
    13621 tacatcgcag tggtcaagca gatcggggag gtgtcgtcgg gcggctacct gttgatcttc
    13681 tggatgttcc agaacccgcc cgacgtgctg tacagcctcg tcaagggcat ggcgatggcg
    13741 gtcgtcgtgg tgctcgtcgg ctgctactac ggctacaacg cgagcggcgg gcccgtcggc
    13801 gttgggaccg cgacggcgaa gtcgatggtg ctcaacacgg tcgcggtgca cctcgtgggc
    13861 atgatgggca cacagctctt ctggggtgct aagccgcgcg cgccgatcgg gggttgaccg
    13921 gcgtgcgacg ccgcacaccg ccgccgcatg cgccgtgggg cgccttcccg ctctccgagc
    13981 tgtgcgtgct gctgtcgctt atgtgcgccg cggcgtcgct cgtgctctgg ggcaccgcac
    14041 ggggcttgtg gctcgtggcg gccgcgggcg tactgggctg cctcgcgggc tgcgaggtgg
    14101 cctaccgcga gcacttcgcc ggctaccgct cgcacaccgc tccgctcgcc ggcagcgtgg
    14161 ccatcccgct cgcggtcggg ctcgtgttcg cgggtatgcc cgccatcgtg gtgctggtgt
    14221 cgggtggcgc cgccttcctc gcggcggtgc ccctgttcga acgcgccttc aggcgccggg
    14281 ccggcctcta gccgccgagc cactcgggcc gcggcccgcg ctggtcgagc accgagaagt
    14341 cgccctcgtc ccgaatgcgc tccgccgccg tgacgagccc gccgagcgct tgccacgcga
    14401 gcgcgccgcc tacgcttacc cgctgaccgc cggcgtcgac gatctccggc atcgtcagtc
    14461 ctcggtgcgc cagcacgttg agcggcttcg aggtcgcctc gctcacggcc cgtatctcgt
    14521 cgccgctccg gagcccgggc gcgtagagca cgtcggcgcc cgcgcgctcg tacgactgca
    14581 gccgcgcgat cgtgtcgtcc aggtccgggt tgccgcggat gtggttctcc gcccgcgccg
    14641 tgagcgtgaa ggggaagtcg agcgcgcgtg ccgcctcgac cgcagcggcg acccgctcgg
    14701 tcgcctcctc gatcggatag agctcgccgg aggggtccca gtcctcgatc gagccgccga
    14761 cggcgccggc ctcggccgcg ctcctgatcg cggtggccgc ctcctcgggg gagcggccgt
    14821 agccgttctc gaggtccgct gagatcggaa cgttcgtggc gctcgcaagc acgctgatgt
    14881 gcctcacgac ctgctctaga gtgaccccgc cgtcgaggcg cccgagcgtg aacgcgaagc
    14941 ccgagctcgt ggtggcgagc gccttgaacc cgagcgcctc gagcacgcgc gccgaacccg
    15001 cgtcccacgg attcgggatc acgaacggct cgccctcgtg cagggcgcgg aaagcgcggg
    15061 ccttctcttc ctgggtggcg ggcattcgcg cagtatgccg gtcagtcgtc gggaagcagc
    15121 ggcaccgagg gtccgtggtc gcgtcccagc agcaccgcgc gggtgaccgc gcccgcgccg
    15181 aagcggtcgc gcagctcgtc aagagtggtg tcgagcgccc acgcgcgctc gcgctcaaag
    15241 ggcagcgcga gctgcactgc cccgccgtcc gagaggttgc caagggcgat cccgagcagc
    15301 gtgattccgc gatcctcgat catcggcagc gccgctgcga gcagctcgcg gccggtggcg
    15361 aggacggttt cggtctcgga ggtcgcctgg agcagcgtgt gcgaacgcgt gacgcgcgag
    15421 aagtcgtcga agcgcagccg cagcacgacc gtgcggcata cgcggcgcgc agcccgtagg
    15481 cgccgtgtga gcttgtcgac cagacccacc agcacagcgt cgagcgcctc gggcgacttg
    15541 ggcgcgcgcc ttcccagcgc gtgctgcgcg cccatcgagc gtcttcgctt gcgtcgctcg
    15601 actcgccgcg ggtcgcggtt gtgcgcgagc gcatgcaagt gccggccgga tgccttcccg
    15661 agcatctcga ccaaatcagc ctcggacagc cgcgcaacct cgccgaccgt ctcgagcccg
    15721 cgctcacgca gcttcgtcga ggtgaccgcg cctacgcccc acaagcgctc gactggaagc
    15781 gggtgcagga acgcgagctc gccggccggg gggacgacga gcatcccgtc gggcttcgcc
    15841 acgccgctcg ccaccttcgc gaggaacttg gtgcgcgcga cgccgaccgt gatcgggatc
    15901 ccgacgcgct cgcgcactgc gcggcggagc ttcacggcga tctcggcggg cgtcccggag
    15961 atgtgctcga gtccgcgcac gtcgaggaac gcctcgtcga tcgagagccc ctcgacgagt
    16021 ggagtcgtgt ccgcgaagac gcggaacatc gcgcggctcg cctcggtgta cgcgttcatc
    16081 cgcggcggga cgaccacggc tcgcgggcag agcc
//
LOCUS       DASVXS010000129         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_317000, whole genome shotgun sequence.
ACCESSION   DASVXS010000129 DASVXS010000000
VERSION     DASVXS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317000"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF752_07140"
     CDS             <1..710
                     /locus_tag="VF752_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX8855361.1"
                     /translation="DHATVAEEEYLQTIYWLEEARLPITGANIARAMQLSAPTVHEMV
                     GRLESDGYVTRNEDKSLEFTDRGREHASAVVRRHRLIERFLTDVLGIPWDEVHEEAER
                     LEHAMSPVLEERMWVAIGDAKTCPHGHPIVEGAREQGVPLADVEKGANVVVLRFENEA
                     EDLLHYLKDSGLYPGFEGTLASGRGDEDVVVESDGKEYTVTQSVAETVAVRADPSPPP
                     RTALPDKLVLTKDRFGR"
     gene            700..1176
                     /locus_tag="VF752_07145"
     CDS             700..1176
                     /locus_tag="VF752_07145"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8855362.1"
                     /translation="MAAESRLVASTVIQAPPEKVWEVVSATGRYAEWVVNTDEVVRTD
                     NPRGGPDVTYDERNTIAGPWKASTRWRVTEFDPPHRQVHEGEGVAFAKGVRLEIDLAP
                     AGESTEISNTAVYEPKFGPVGALLDKALRPMLERDFRRTMENLKRLVESEANVATR"
     gene            complement(1173..1925)
                     /locus_tag="VF752_07150"
     CDS             complement(1173..1925)
                     /locus_tag="VF752_07150"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEX8855363.1"
                     /translation="MSEREPLDLNRPRSAGELLGDSLGIYFRNFLMLVGAAATVVVPI
                     ELILVGFGLKQFTADPDAALSSGAQVMDVLVMYAVVAPLVTAIAVALLKGLGNGQRPR
                     FGPTVVGALEAFTPLFITLLIAAAGISIGLILFIVPGVYLAVRWTFASQAVLLDRVRG
                     AQALQRSGELTTGNWWRVFGVLLLAGLVAVIATAAVGLPFELLAHSADSGALRLTGLI
                     IVRTLTTPFLAVMITLLYFDLRARRGVAAAVV"
     gene            complement(1922..3025)
                     /locus_tag="VF752_07155"
     CDS             complement(1922..3025)
                     /locus_tag="VF752_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8855364.1"
                     /translation="MSSQPPITFARGAPSLDIIAVDDLKAAAERAFSEDPAGSFSYGT
                     ALGYTPLREWIAQKQELALEQVITTNGSMQADAFLFELVVQPGDVVVVEAPTYDRTLL
                     ALRKLGAQLLAVPLEADGVDVGALEAALKGGARPKLAHIIPNFQNPAGCTLSLEKRRR
                     LVELASEYEFTIFEDDPYVELRFEGDPLPTMLSLDHADRVVYASSFSKTVCPGIRVGY
                     LAGPAPDIAQVQKLATETYISPSMVSQAIVAEFCRSGAIERSIETVKKALHERVDALT
                     SSLESELPEARFVKPQGGYFLWVELPEGSDTDLLAAAAAERGVMFVKGSDFLVEGGKN
                     ALRLAYSGVGPDEIREGVRRLAEAYHELGRAAA"
     gene            3119..3673
                     /locus_tag="VF752_07160"
     CDS             3119..3673
                     /locus_tag="VF752_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855365.1"
                     /translation="MAAVPMEHLVAAVRALDPGGRALLDLSLRRGVTDHEIARVLHTH
                     PAEVALRRARVLDRLAGNVGVNGSADLLETALNALPAEIWLLSAPVVEEAPPRPPLRS
                     LPAPPEESPPQVEPPAPELGRPTPPPPPLRPTPPAPDYRWALPPEPEQRPTHALRKVA
                     FGGVAVAVLAAAAGVAVGRRLRGR"
     gene            complement(3681..4559)
                     /gene="sucD"
                     /locus_tag="VF752_07165"
     CDS             complement(3681..4559)
                     /gene="sucD"
                     /locus_tag="VF752_07165"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX8855366.1"
                     /translation="MSVLVDKDTKLVISGLTGREGRFHGLNNRNYGTNVVAGVTPGKR
                     GQEVEGIPVFDTVRDAVSETGANTSMIFVPPRFAADSILESIDAGIELVIAITEGIPV
                     HDMLRVYTQLKRTSSRLVGPNCPGILSPGRANVGIIPAHFFSEGNVGLVSRSGTLTYQ
                     IGNELAQQGFGNSSIVGIGGDPIAGSSFIDIIELFEGDPETELIVMCGEIGGEEEERT
                     ADYIAEHVTKPVVGYIAGFTAPPGKTMGHAGAIISGSKGTAAAKAEALESKGVKVGRT
                     PTEVAEIAVSLLGARA"
BASE COUNT          734 a         1620 c         1611 g          679 t
ORIGIN      
        1 ccgatcacgc gaccgtcgcc gaagaggagt atctgcagac catctactgg ctggaggagg
       61 cgcgcctgcc gatcaccggc gcgaacatcg cccgcgcgat gcagctctcc gcgccgaccg
      121 tgcacgagat ggtcggccgc ctcgagtccg acggctacgt gacccgcaac gaggacaagt
      181 ccctcgagtt caccgacagg ggtcgcgaac atgcgagcgc ggtagttcgg cgccatcggc
      241 tgatcgagcg attcctcacc gacgtcctcg gcatcccctg ggacgaggtg cacgaggagg
      301 ccgagcggct cgagcacgcg atgtcgcccg tgctggagga acgcatgtgg gtcgcgatcg
      361 gcgacgcgaa gacctgcccg cacggccatc ccatcgtcga aggcgcgcgc gagcagggcg
      421 tgccgctcgc ggacgttgag aagggcgcga atgtcgtcgt cctgcgcttc gagaacgagg
      481 ccgaggacct gctccactac ctgaaggact cagggctata cccgggcttc gagggcacgc
      541 tcgcgagcgg ccgcggcgac gaggacgtcg tggtcgagtc ggacggcaag gagtacacgg
      601 tcactcagag cgtggccgag accgtggcgg tgcgcgccga cccctcgcca ccgccgcgca
      661 cggcgctgcc cgacaagctg gtgctgacga aggatcgctt tggccgctga gagccgactc
      721 gtcgcatcga cggtgatcca ggcgccgccg gagaaggtct gggaggtggt gtcggccacc
      781 ggccgatacg cggagtgggt cgtgaacacc gacgaggtcg tgcggaccga caacccgcga
      841 ggcggtccgg atgtcaccta cgacgagcgc aacacgatcg ctgggccgtg gaaggcgagc
      901 acccgctggc gcgtgaccga gttcgacccg ccgcaccggc aggttcacga gggcgagggc
      961 gtcgcgttcg cgaagggagt gcggctcgag atcgacctcg cgcccgcggg cgagtccacc
     1021 gagatcagca acacggccgt gtacgagccg aagttcggcc cggtcggcgc gctgctcgac
     1081 aaggcactga ggccaatgct cgaacgcgac ttccgaagga ccatggagaa cctgaagcgc
     1141 ctcgtggagt ccgaggcgaa cgtcgcgacc cgctagacga ccgccgccgc gaccccgcgc
     1201 cgggcgcgca ggtcgaagta gagcagcgtg atcatcaccg cgaggaacgg cgtggtgagc
     1261 gtgcggacga tgatcagtcc ggtgagcctg agcgcgccgg agtcggccga gtgcgcgagc
     1321 agctcgaacg gcagtccgac cgccgccgtc gcgatcaccg ccacaaggcc ggcgagcagc
     1381 agcaccccga acacccgcca ccagttgccc gtcgtgagct cgccactccg ctgaagtgcc
     1441 tgggcgccgc gcacgcgatc gagcaggacg gcctggctcg cgaacgtcca gcgcacggcg
     1501 aggtagacgc cgggcacgat gaacaggatc aggccgatcg agatgccggc ggccgcgatc
     1561 agcagcgtga tgaacagggg cgtgaaggcc tcgagcgcgc ccacgacggt tggcccgaac
     1621 ctcgggcgct ggccgttgcc gaggcctttg agcagcgcga ccgcgatcgc cgtgacgagc
     1681 ggcgcgacca ccgcgtacat caccagcacg tccatcacct gggcgccgct cgagagcgca
     1741 gcgtcgggat cggcggtgaa ctgcttgagg ccgaagccca cgaggatcag ctcgatcggc
     1801 acgacgaccg tggcagccgc gcccacgagc atcaggaagt tccggaagta gatgccgagg
     1861 gagtccccga gcagctcacc cgcgcttcgc ggccggttga ggtcgagcgg ctcgcgctcg
     1921 ctcacgcggc cgcgcggccg agctcgtgat acgcctcggc cagcctgcga acgccctcgc
     1981 ggatctcgtc gggtccgacg cccgagtagg cgagccgcag cgcgttcttc ccgccctcga
     2041 cgaggaagtc gctgcccttc acgaacatca cgccccgctc agcggcggcc gcggccagca
     2101 ggtcggtgtc gcttccctcg ggcagctcga cccagaggaa gtagccgccc tgcggcttca
     2161 cgaaccgcgc ctccggaagc tcgctctcga gcgatgaggt gagcgcgtcg acccgctcgt
     2221 gcagcgcctt cttgaccgtc tcgatcgacc tctcgatcgc gcccgagcgg cagaactccg
     2281 cgacgatcgc ctgcgacacc atgctgggcg agatgtatgt ctcggtggcg agcttctgga
     2341 cctgagcgat gtccggggcc ggccccgcga ggtagccgac gcggatgccg gggcagaccg
     2401 tcttcgagaa cgacgacgcg tacacgacac ggtccgcgtg gtcgagcgac agcatcgtcg
     2461 gcagcgggtc gccctcgaag cgcagctcca cgtaggggtc gtcctcgaag atggtgaact
     2521 cgtactcgct cgcgagctcc accagccgcc gccgcttctc tagcgagagc gtgcagccgg
     2581 ccgggttctg gaagttgggg atgatgtgcg ccagcttcgg ccgagcgccg cccttgagcg
     2641 ccgcctcgag cgcacccacg tctacgccgt cggcctcgag cggcaccgcg agcagctgag
     2701 ccccgagctt gcgcagcgcg agcagcgtgc ggtcgtacgt gggcgcctcg accacgacca
     2761 cgtcgcccgg ttgaaccacc agctcgaaca ggaaagcgtc ggcctgcatc gagccattcg
     2821 tcgttatcac ctgctcgagc gcgagctcct gcttctgcgc aatccactcg cgcagcggcg
     2881 tgtaccccag cgccgtgccg taggagaagc tcccggccgg gtcctcactg aaggcgcgct
     2941 cggccgcggc cttcaggtcg tcgaccgcga tgatgtcgag tgatggcgct ccacgcgcga
     3001 aggtgatcgg gggctgcgag ctcacggcac cgcacgataa cgccaacaag cgcggggcga
     3061 ccgagccgcg gggtagcatc gcgctgcggg tctctgcgcc ccgcggggta ctcgtgccat
     3121 ggccgccgta ccaatggagc atttggtagc cgcagtccgg gcgctcgacc cgggcggtcg
     3181 cgcgctgctc gacctgtcgc ttcgcagggg cgtcacggac cacgagatcg cccgcgtgct
     3241 tcacacgcat ccggccgagg tcgcgctgcg ccgcgcgcgc gtcctcgatc gcctcgccgg
     3301 gaacgtcggc gtgaacggct ccgccgatct cctggagact gcgctcaacg cgctgccggc
     3361 ggagatctgg ttgctctccg cgccggtggt ggaagaggcg ccaccgcgtc ctccgcttcg
     3421 gtcgcttccc gcccctcccg aggagtctcc accgcaggtt gagcctcccg cgccggagct
     3481 gggacgaccc acgccgccgc caccaccatt acgccctacg ccgcctgcac cggactaccg
     3541 ctgggcgctt cctcctgagc cggagcagcg accgactcat gcgctccgca aggtcgcctt
     3601 cggcggcgtc gcggtggccg tgctcgcggc ggccgcgggc gtggcggtgg gccgccgtct
     3661 ccgcgggcgc tgagttcgtc ctaagccctg gcgccgagca gcgagaccgc gatctccgcg
     3721 acctcggtgg gcgtgcggcc caccttcacg cccttggact cgagcgcctc tgccttcgcg
     3781 gcggcggtgc ccttcgagcc cgagatgatc gccccggcgt ggcccatcgt cttgccgggc
     3841 ggcgcggtga agccggcgat gtaacccacc accggcttcg tcacgtgctc ggcgatgtag
     3901 tcagcggtgc gctcttcttc ctcgccgccg atctcgccgc acatcacgat caactcggtt
     3961 tcggggtcgc cctcgaacaa ctcgatgatg tcgatgaacg acgagcccgc gatcgggtcg
     4021 ccgccgatgc cgacgatgga cgagttgccg aagccctgct gagcgagctc gttgccgatc
     4081 tggtacgtca gcgtgccgga acgcgaaacc aggcccacgt tgccctcgct gaagaagtgc
     4141 gcggggatga tccccacgtt ggccctgccg ggcgacagga tgcctgggca gttcgggccg
     4201 acgagccgcg agctcgtgcg tttgagctgg gtgtacacgc ggagcatgtc gtgcacgggg
     4261 atgccctcgg tgatcgcgat tacgagctcg atgccggcgt cgatcgactc gaggatcgag
     4321 tccgcggcga agcgcggcgg cacgaagatc atgctcgtgt tagcgccggt ctcggatacg
     4381 gcgtcgcgca cggtgtcgaa gacggggatg ccctcgacct cctggccgcg tttgccgggg
     4441 gtcacgcccg cgaccacgtt cgtgccgtag ttgcggttgt tcagcccgtg gaacctgccc
     4501 tcacggccgg tcagcccgga gatgacgagc ttggtgtcct tgtctacgag aacgctcact
     4561 tggcgagctc caccaccttc tgcgcggcgc cgagcatggt cttctcgacg tgcacgttgg
     4621 gtaggttcgc ctccgcgagg atac
//
LOCUS       DASVXS010000130         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_389674, whole genome shotgun sequence.
ACCESSION   DASVXS010000130 DASVXS010000000
VERSION     DASVXS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389674"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..1365
                     /locus_tag="VF752_07170"
     CDS             532..1365
                     /locus_tag="VF752_07170"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8855367.1"
                     /translation="MFVFDPGMVVLLALSIGLYWRAVRVLRRRGYRVPSGQQVCWYAG
                     VACLAIGLLGPPDALADDLFVAHMGEHLLIADIAGPLLLFGLRTPVLVFFLPKPILVP
                     LAHRKRLRAAFRFLRRPLVAIPVFALVLYGWHFAGPFDAALRNPSLHALQHLSFVAAS
                     MLVWWSAVEPQRRHMSGELWKIGYILGARIISMFLATALIFARSPFYSYYGDKPREHG
                     LSPLTDQQVGGGLMLGLDLLVMLFALSFFFWSAAKEYDRREAQQQQQQQQQPTSPAAT
                     G"
     gene            complement(1362..1685)
                     /locus_tag="VF752_07175"
     CDS             complement(1362..1685)
                     /locus_tag="VF752_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8855368.1"
                     /translation="MTTEPARLTLYTCHIDRGGPPVHPCRRAHEALDEAGIDYETVIF
                     DRNRPFGLFTKGRRPELRQMSGQEKLPVLRLPDGSTVNGSGAIVRWARDNAPAGADSS
                     VTTSS"
     gene            complement(1716..2210)
                     /locus_tag="VF752_07180"
     CDS             complement(1716..2210)
                     /locus_tag="VF752_07180"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX8855369.1"
                     /translation="MEPRRPSDSRSELTRWMGVTDANTAGNVHGGAIMRLCDEVAGLA
                     AIRHSGAKVVTAGMDRMTFMHPVYVGNLVTVRATVNAAWRTSMEVGVRVESEDVRTGI
                     RRHTSSVYLTMVALDDDGRPTEVPPLEPETDEQRRRQREAQLRRDVRLSEREQILAGR
                     GGVD"
     gene            2288..3022
                     /locus_tag="VF752_07185"
     CDS             2288..3022
                     /locus_tag="VF752_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX8855370.1"
                     /translation="MSTVVKAKISKLLDRAEDPSETLEYGYQKQLELLQNVKKGIADV
                     TTSKKRLQMQSEQLEQQVVKLDTQARQALSQNREDLARVALERKTVAPTELQSLDQQV
                     KDLEAQQQSLTDNEAKLRSKIEAFRTKKEVIKAQYSAAEAQVRISEAATGVGEQMADL
                     GLAIQRAEDKTENMRARAQAVDELEKAGTFEDLTQLGPGQDDIDRQLSQLGQESQVDD
                     ELAKMKAELGSGGGESPALGQGGQQQ"
     gene            3019..3297
                     /locus_tag="VF752_07190"
     CDS             3019..3297
                     /locus_tag="VF752_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855371.1"
                     /translation="MIVRVSGEGQYELPETDAERLNELDNQAVAAVEAGDEDRFSELW
                     HQMLELVQNDGRELADDELVESDVILPPRDITFEEASNDFSGEGLIPD"
     gene            3373..3798
                     /locus_tag="VF752_07195"
     CDS             3373..3798
                     /locus_tag="VF752_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855372.1"
                     /translation="MSTAPNGIERPLIACRYPDADVAREAWERLSSYFSANKFMAISS
                     MRCVGRSDHGETHLVVAAGFTEPDGWVLDGGPWGDEAIRCDDALPEPIKDQLYSRVAH
                     AAATLAAEGQSGTFTTRRPHGARILIDADGNVRPESNAG"
     gene            complement(3902..4471)
                     /locus_tag="VF752_07200"
     CDS             complement(3902..4471)
                     /locus_tag="VF752_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855373.1"
                     /translation="MGFLDAILGGRRKLTQPAPDRLFAMTTCQVTLETGLGMKHRDVA
                     GIVFQPLPTADFQQIVKDTEALLRSAAEDTGTKVETADDEFGYSWIILRDEDFEDLVV
                     AMNLVSSQLQAGGYGDRLLAAVFVFEENGRPIYFIYNFKRGAYYPFVPTGDKQRDNER
                     EFRLKAQLSGELPIEPEVERWFPLWGTPL"
     gene            complement(4476..5390)
                     /gene="htpX"
                     /locus_tag="VF752_07205"
     CDS             complement(4476..5390)
                     /gene="htpX"
                     /locus_tag="VF752_07205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX8855374.1"
                     /translation="MARPKASFGRDPGLQARMLFTIFLLGLLYVAFVGVLIAAGAGAI
                     TILIVIGALSLAQLFLSDKIGLAAMGAKEVSPAEAPGLHAMIERLCIQADLPKPKVAV
                     ADTDVPNAFAMGRNQKAAVVCCTTGIMNVLEPAELEGVLAHELTHVKNRDVMIMTIAS
                     FFASIAATITQFGFFFGGGFGGDDDDGAPAFIVVLLVSFAVYILSFFLMLALSRYREF
                     AADRGSALITGRPSALASALVKISGAMQRVPDQDLRQVGRMNAFFIVPTSVKQSVATL
                     FASHPPIEARIARLEQYERELQGTALAA"
     regulatory      5520..5665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            5667..>6023
                     /locus_tag="VF752_07210"
     CDS             5667..>6023
                     /locus_tag="VF752_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855375.1"
                     /translation="MKRAFWVSITLALSAYFLLPMPGLSEPLNKRIERAQQKVAAKKH
                     TEGVLTSDISNYNNKIRGLQGDIRSLQDRQNRLQGQLNQKRAELQRVQNELQIARDRL
                     VRLKTRLNFAEGVLAKR"
BASE COUNT         1006 a         2060 c         2006 g          951 t
ORIGIN      
        1 gcctgtggag tcggggccgt tccccttcgg gctcccttcg cgcttcgcgc tcactcttga
       61 cgcccttcgg ggaaggcccc tcctcccagg cctacgccag cgccgcgcca gccgactcgc
      121 tttgagaagc aggacccgcc gccccggggt tcaaacgttc catctcgccg ccggcccagc
      181 gtcgagatgg aggttgtggt ccgtatgggt ccatttcctc catctcgctt ccgcgaatcg
      241 ctcgagatgg aagttatgca cccctcgcgg tctcccctta gtgcttaacc gagccgccgg
      301 ggcgctcgag ggcgaccacc ggcgtcgcgg ccaggggctc gagcgggaag aagttccatc
      361 tcgatcccgt tcccgcgacg ccatggaagt ttgtcccgct atgcgggaag gacttccatg
      421 ccgcctggta cggatgcctg ggatggaggt ttttcccgct gctgttccga gggccctgac
      481 gcccccgcat taatcgcgac tcaacgcgaa cacgcgtcac aatcacgcgt gatgtttgtc
      541 ttcgaccccg ggatggtcgt ccttctggcg ctgtcgatcg gcctgtactg gcgcgctgtc
      601 cgggttctcc gcagacgcgg ctaccgcgtt ccgagcgggc agcaggtctg ctggtacgcc
      661 ggggtcgcct gcctcgcgat aggcctgctc ggcccgcccg acgcgctcgc cgacgacctc
      721 ttcgtcgcgc acatgggcga gcacctgctg atcgccgaca tcgccgggcc gctcctcctg
      781 ttcggactcc ggacgccggt gctcgtgttc ttcctgccga agccgatcct cgtgccgctc
      841 gcgcatcgaa agcgcctgcg cgccgccttc cgcttcctcc gccgcccgct cgtcgcgatc
      901 cccgtgttcg cgctcgtcct atacggctgg cacttcgcgg gcccgttcga cgccgccctc
      961 cggaacccgt cgctgcacgc gctccagcac ctctcgttcg tggcggcgag catgctcgtg
     1021 tggtggtcgg cggtcgagcc gcagcggcgc catatgtcgg gcgagctctg gaagatcggc
     1081 tacatcctgg gcgcgcgcat catcagcatg ttcctcgcga ccgcgctgat cttcgcccgc
     1141 agtccgttct actcgtacta cggcgacaag ccccgcgagc acggtctctc gccgctcacc
     1201 gaccagcagg tgggcggcgg tctcatgctc ggactcgacc tgctcgtgat gctgttcgcg
     1261 ctctcgttct tcttctggag cgcggcgaag gagtacgacc gccgggaggc tcagcagcag
     1321 cagcagcagc agcagcagcc gacgagcccc gcggcgaccg gctagctgct cgtcgtcacc
     1381 gacgagtccg cgccggccgg cgcgttatcc cgcgcccagc ggacgatggc gcccgagccg
     1441 tttacggtcg aaccgtccgg cagccggagc accggcagct tttcctgccc ggacatctgt
     1501 cgcagctcgg gccggcggcc cttggtgaac aggccgaagg ggcggttgcg gtcgaagatc
     1561 acggtctcgt agtcgatacc ggcctcgtcg agcgcctcgt gcgctcgccg gcagggatgc
     1621 accggcgggc ctccgcgatc gatgtggcac gtgtagaggg tcaggcgcgc gggctcagtc
     1681 gtcatacgac taagaatggc aggcgctgtc gcgcatcagt ccacgccgcc gcgcccggcc
     1741 aggatctgct cgcgctcgga gagccgcacg tcgcgccgca actgcgcctc gcgctgccgc
     1801 cggcgctgct cgtccgtctc gggctcgagc ggtggcacct cggtggggcg gccgtcgtca
     1861 tcgagcgcga ccatcgtgag atacacgctc gacgtatggc gccggatgcc cgtcctcacg
     1921 tcctcggact cgacccgcac gccaacctcc atcgacgtgc gccaggccgc gttcacggtt
     1981 gccctcacgg tcacgaggtt cccgacgtac accgggtgca tgaacgtcat gcggtccatg
     2041 cccgccgtca cgaccttcgc acccgagtga cgaatggccg cgagcccagc cacctcgtcg
     2101 cagaggcgca tgatcgcgcc gccgtgcacg ttgcccgccg tattcgcgtc ggtcacgccc
     2161 atccagcgtg tcagctcgga ccgcgagtct gaggggcgtc gtggctccac ggcggcagta
     2221 gtctagaagc ccctcgtccg acccaaaatg tgaaggaggc gtgcccatgc caggcctggc
     2281 aggacgcatg tcgacagtag tgaaggcaaa gatctcgaag ctcctcgacc gcgcggagga
     2341 tccgtccgag actctcgagt acggctacca gaagcagctc gagctgctgc agaacgtgaa
     2401 gaagggcatc gccgacgtga cgacgtccaa gaagcgcctg cagatgcaga gcgagcagct
     2461 cgagcagcag gtcgtgaagc tcgacaccca ggcgcgacag gcgctgtcgc agaaccgcga
     2521 ggatctcgcg cgcgtcgcgc tcgagcgcaa gacggtcgcg ccgaccgagc tccagtcgct
     2581 cgaccagcag gtgaaggacc tcgaagcaca gcagcagtcg ctcaccgaca acgaggcgaa
     2641 gctgcgctcg aagatcgagg ccttccgcac caagaaggaa gtcatcaagg ctcagtactc
     2701 cgcggccgag gcgcaggtgc gcatctcgga ggcggcgacc ggagtgggag agcagatggc
     2761 cgatctcggc ctcgcgatcc agcgtgccga ggacaagacc gagaacatgc gcgcgcgtgc
     2821 ccaggcggtg gacgagctcg agaaggccgg cacgttcgag gacctcacgc agctcggccc
     2881 cggacaggac gacatcgatc gccaactctc tcagctgggg caggagtcgc aggtggacga
     2941 cgagctggcc aagatgaagg ctgagctcgg atcgggtggc ggcgagtcgc cggccctcgg
     3001 gcagggcggg cagcagcagt gatcgtccgc gtgtcgggcg agggtcaata cgagctgccg
     3061 gagaccgatg ccgagcggct gaacgagctc gacaaccagg ccgttgcggc ggtcgaggcg
     3121 ggcgacgagg accgcttcag cgagctctgg caccagatgc tcgagctcgt acagaacgac
     3181 gggcgcgagc tggcggacga cgagctcgtc gagtccgacg tgatcctgcc tccgcgggac
     3241 atcacattcg aagaggcgag taacgacttc tcgggcgagg gcctcatccc tgactaggcg
     3301 tttctggacg ctctcggcgt atggccgagg gcgtccttga caccccgcgg cgcgggggta
     3361 ccgtgaacgc agatgagcac cgcgccgaac ggaatcgagc gaccgctgat cgcctgccgc
     3421 tacccggacg cggacgtggc acgcgaggcg tgggagcgcc tctcgtcgta cttctcggcg
     3481 aacaagttca tggcgatctc gtcgatgcgc tgcgtcggcc ggagcgacca tggcgagacg
     3541 cacctcgtgg tggcggccgg cttcaccgag cccgacggct gggtgctcga cggtggtccc
     3601 tggggcgacg aggcgatccg ctgcgacgac gcgctacccg aaccgatcaa ggaccagctc
     3661 tactcgcggg tcgcgcacgc ggccgcgacg ctcgcggcag agggccagtc aggcactttc
     3721 accactcgac gcccgcacgg cgcgcgcatc ctgatcgacg ccgacggcaa cgtccggccc
     3781 gagagcaacg ccggctgaga tcaggagccg gtcgccagct gctgcgtgtc cgctgagcca
     3841 cagtcgccgg tgcacggtgc cggctcagcg accgagcgac tcgacatgag tggctagggc
     3901 tttagagagg cgtgccccag agggggaacc agcgctcgac ctcgggctcg atcggcagtt
     3961 cgccgctgag ctgcgccttc agccggaact cgcgctcgtt gtcgcgctgc ttgtcgcccg
     4021 ttggtacgaa cgggtagtac gcgccgcgct tgaagttgta gatgaagtag atcggacggc
     4081 cgttctcctc gaacacgaac actgccgcca gcagccggtc gccgtagccc ccggcctgga
     4141 gctggctcga gacgaggttc atggcgacta cgaggtcctc gaagtcctca tcgcgcagga
     4201 tgatccagct gtagccgaac tcgtcgtcgg cggtctcgac cttcgtgccg gtgtcctccg
     4261 ccgcggagcg gagcagcgcc tcggtgtcct tcacgatctg ctggaagtcc gccgtcggca
     4321 gcggctgaaa gacgatgcct gccacgtcgc ggtgcttcat cccgaggccc gtctcgagcg
     4381 tcacctggca ggtggtcatc gcgaagagcc ggtcgggcgc cggctgcgtg agcttgcggc
     4441 gcccgccgag gatggcgtcg aggaatccca agtcgctacg cggccagcgc cgtgccctgt
     4501 agctcgcgct cgtactgctc gagccgggcg atgcgggcct cgatcggcgg gtggctcgcg
     4561 aacagcgtgg ccaccgactg cttcacgctc gtcggcacga tgaagaaggc gttcatccgg
     4621 cccacctggc ggaggtcctg atcgggcaca cgctgcatgg cgccggagat cttcacgagc
     4681 gccgaggcaa gcgcgctcgg acgccctgtg atcagcgccg agccacggtc cgccgcgaac
     4741 tcgcggtagc gcgaaagggc gagcatcagg aagaagctga ggatgtatac ggcgaagctg
     4801 acgaggagca ccacgatgaa cgcgggcgcg ccgtcgtcat cgtcaccgcc gaagccaccg
     4861 ccgaagaaga acccgaactg cgtgatcgtc gctgcgatag acgcgaagaa gctcgcgatg
     4921 gtcatgatca tcacgtcgcg gttcttcacg tgcgtgagct catgcgcgag cacgccctcg
     4981 agctcggccg gctccagcac gttcatgatc ccggtcgtgc agcacaccac cgcggccttc
     5041 tggttgcggc ccatcgcgaa agcgttcggc acgtcggtgt cggccaccgc gaccttcggc
     5101 ttcggcagat ccgcctggat gcagaggcgc tcgatcatcg catggaggcc cggcgcctcg
     5161 gccggcgaga cctccttcgc tcccattgca gcgagcccga tcttgtccga gaggaagagc
     5221 tgggcaagcg acagggcgcc gatgacgatg aggatcgtga tcgcgcccgc acctgctgcg
     5281 atgagcacgc cgacgaaggc cacatagagc agccccagca ggaagatcgt gaacagcatc
     5341 cgcgcctgga gtccaggatc gcggccgaat gaagccttgg gtcgagccat gcattgattg
     5401 tagttagggg cgggtggctc tccccgtcgc gctgcgggca cgattctcct cttcgctgca
     5461 accgcggttg cagaaccgcg tgacccgccg catcccgctc cacaactgat aatctcgccc
     5521 gcgtccaatc gctgaattcc cctgcatacc aggggaaacg ggggacccaa cagtcgatgt
     5581 ccggggcgaa tcgccgtctg ccaggcggcg tagcgtcagc acgcgagccc gtcagctaac
     5641 cccgtaagcg cagcggacca gacgcaatga agagagcttt ctgggtctcc ataacgctgg
     5701 ccttgtcggc ctacttcctg ctgccgatgc ccgggctctc cgagccgctc aacaagcgga
     5761 tcgagcgcgc gcagcagaag gtggcggcca agaagcacac ggagggcgtg ctcacctccg
     5821 acatctcgaa ctacaacaac aagatccgcg ggctccaggg cgacatccgc agcctgcagg
     5881 atcggcagaa ccggctgcag ggccagctga accagaagcg cgccgagctg cagcgggtcc
     5941 agaacgagtt gcagatcgcg cgcgatcggc tcgtgcgcct gaagacgcgc ctcaacttcg
     6001 ccgagggtgt gctggcgaag cgg
//
LOCUS       DASVXS010000131         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1379977, whole genome shotgun sequence.
ACCESSION   DASVXS010000131 DASVXS010000000
VERSION     DASVXS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379977"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF752_07215"
     CDS             <1..255
                     /locus_tag="VF752_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855376.1"
                     /translation="RYGSITVVDAGSAGAGGVFGARSTPVGVGLLYLEPNGKLPNAID
                     MVQVEPFSGAANAGRVLLDSKTVCDSRAVMCHDTGAKPPG"
     gene            complement(432..1370)
                     /locus_tag="VF752_07220"
     CDS             complement(432..1370)
                     /locus_tag="VF752_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855377.1"
                     /translation="MAQVQTTAGPLDVESLGLTLVHEHLRIRSEQVVAQFPHIYDDDH
                     EFERAVDQVNRAKALGVTTICEPTVMGNGRDARFAQRVAEETGMQIVLSTGIYTYHYL
                     PAHFQNRDEDYMAELFVRDIEIGIQGTDVKAGFLKCCTDEHGMTPDVEKVLRAVARAS
                     KQTGRPIMTHSHPASGSGLQQMGIFMEEGVDPAKVMIGHTGDTDSLHYIETLLAMGPY
                     IGMDRYGIDIILPTERRNATLLELVRRGYADRMFISQDTCCTIDWYPEELVAEMAPNW
                     HMGYVLGEVIPGLMEQGLEQSAVDQMLLENPKRWLG"
     gene            complement(1399..1965)
                     /locus_tag="VF752_07225"
                     /pseudo
     CDS             complement(1399..1965)
                     /locus_tag="VF752_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(2031..3029)
                     /locus_tag="VF752_07230"
     CDS             complement(2031..3029)
                     /locus_tag="VF752_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855378.1"
                     /translation="MNAGELRTRPRFPDVPREAGRYESFYLKASHPTEPLGFWIRHTV
                     HKRPGSEPKGSLWFVLFTADAQHASKTTLPASEVGASEGEYIHIGDSVLTPGRARGSA
                     RTDRLDASWDLTFESPEPPLYHLPRDWMYERSLPRTKSLSPYPAARFSGTLQAGDKRV
                     EVDGWRGMVGHNWGAEHAERWIWLQGSGFEGHEDDWLDLTIGRVRLGPLTTPWIANGA
                     VSIGGERHRVGGIERVRKTEVAESPTGAELVIPAKDLTLRLSVGAPRECFVAWVYADP
                     AGPEHNAVNCSIAGLTATAERRGGEPLRLATAHGAAYELGMRETDHGIPLQPFSDG"
     gene            3065..>3335
                     /locus_tag="VF752_07235"
     CDS             3065..>3335
                     /locus_tag="VF752_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8855379.1"
                     /translation="MADERPVIVITGASSGIGAATARRAAEAGWRLVLGARSEDRLAA
                     LASELGGDEVAIGRRCDVTDWNDQQALAAAALDHFGRLDAMFANAG"
BASE COUNT          471 a         1150 c         1169 g          545 t
ORIGIN      
        1 cgctacggct cgatcaccgt ggtcgacgcg ggcagcgccg gcgcgggcgg cgtgttcgga
       61 gcccgcagca cgccggttgg tgtggggctc ctctacctgg agccgaacgg gaagcttccg
      121 aacgcgatcg acatggtgca ggtcgagccg ttctcgggcg ccgcgaacgc agggcgcgtc
      181 ttgctcgact cgaagacggt gtgcgactcg cgcgcggtga tgtgccacga cacaggcgcg
      241 aagccgcccg gctgaccccg gccggttggt tgtacggtcg tgaaaggcag caaaaaccgc
      301 tgagcgtgaa agattgcgcg cgcaagtgct gactttggtt ttcatatgcg gaaccaaact
      361 cagcagtagc gtggatacgc tcgtgctagc ccttattgct gttgtttcga gccgttcggc
      421 gcgccgcggg ctcagccgag ccagcgcttc gggttctcga ggagcatctg gtccaccgcg
      481 gactgctcga ggccctgctc catcagcccc gggatcacct cgccgaggac gtatcccatg
      541 tgccagttcg gcgccatctc ggcgaccagc tcctcggggt accagtcgat cgtgcagcag
      601 gtgtcctgtg agatgaacat gcggtcggcg tagccccgcc gtaccagctc gagcagcgtt
      661 gcgttgcggc gctcggtcgg caggatgatg tcgatgccgt agcggtccat gccgatgtac
      721 ggccccatgg cgagcagcgt ctcgatgtag tgcagcgagt cggtgtcgcc ggtgtgcccg
      781 atcatcacct tggccgggtc caccccctcc tccatgaaga tccccatctg ctggagcccg
      841 gacccgctcg cggggtgcga gtgggtcatg atcggccggc ctgtctgctt cgacgcccgc
      901 gcgaccgcgc gcagcacctt ctcgacgtcg ggcgtcatgc cgtgctcgtc ggtgcagcac
      961 ttgaggaagc ctgccttcac gtcggtccct tggatgccga tttcgatgtc gcgcacgaac
     1021 agctccgcca tgtagtcctc gtcgcggttc tggaagtgcg ccgggaggta gtggtacgtg
     1081 tagatcccgg tcgagaggac gatctgcatg ccggtctcct cggcgacgcg ctgcgcgaac
     1141 cgcgcgtcgc ggccgttgcc catcacggtc ggctcgcaga tcgtcgtgac gccgagcgcc
     1201 ttggccctgt tcacctggtc cacggcgcgc tcgaactcgt ggtcgtcgtc gtagatgtgc
     1261 gggaactgcg cgaccacctg ctcggagcgg atgcggaggt gctcgtgcac gagcgtcagt
     1321 ccgaggctct cgacgtcaag cggcccggcg gtggtctgca cctgcgccat ccgcgcgatc
     1381 ctaacggtga cgctggcgtc agggaatggt caccggctcg tgcccgagct tctccatgac
     1441 gcgctcgaac acgtcgtgcg gctgcgcgcc gggcacgagc aggcgctcgt cgacgagcca
     1501 cgcgggcacg ccacccgccc cgagttcgag cgcagcctgc gtccattgcg tcacgcgctc
     1561 gtcgagctct gtatcggcga acgcggagcg gccgtcctcc tccgtgatgc ccgcctcgcc
     1621 cgcgaggccg acgagcacgt cgtcgtcgcc gatgtcgcgc gcctccgccc agtacgcgtg
     1681 gaagaggcgc ggatgcagct tgccgaagcg gccgagatcg cgggcgcgct ccccgagctt
     1741 gagcgccttc ttggtgttag ggacgcgctc gatggtgtcc gcgtgcggca gccccgccgc
     1801 gtcgaacatg cgcgcgagct gcgccgtgtg ctcactgccg tagcgcgcga tcagctccgc
     1861 gcgctcgatg ccctcgggcg ggtactcggg atggaggtcg aagggccgcc acgacacctg
     1921 cgcgccgaac cggcgctcga gccacgcggc acgttcggtg ccgacgtagc agaagggtca
     1981 tatgtagtcg gaccagacga ctatgcgcgg gggctccact gccccgcacg ttacccgtcg
     2041 gagaacggct gaagggggat cccgtggtcg gtctcgcgca tgccgagctc gtacgcggcg
     2101 ccgtgcgcgg tcgccaggcg cagcggctcc cctcccctgc gctcggccgt cgcggtcagg
     2161 cccgcgatcg agcagttcac ggcgttgtgc tcggggcccg cggggtccgc gtacacccag
     2221 gcgacgaagc actcgcgcgg cgcgccgacg ctcagcctca gcgtcaggtc cttcgcaggg
     2281 atcacgagct ccgcgcccgt tggggactcc gccacctcgg tcttccgcac ccgctcgatc
     2341 ccacccacgc ggtgacgctc gccgccgatc gagaccgcac cgttggcgat ccacggcgtg
     2401 gtgagcggac cgagcctcac gcggccgatg gtcagatcga gccagtcgtc ctcgtgcccc
     2461 tcgaagcccg agccctggag ccagatccag cgctcggcgt gctcggcgcc ccagttgtgg
     2521 cccaccatcc cgcgccagcc gtcgacctca acgcgcttgt cgccggcctg aagggtgccg
     2581 ctgaagcggg cggcgggata cggcgagagc gacttcgtcc gcggcaacga gcgttcgtac
     2641 atccagtcac gcggcaggtg gtacagcggc ggctccggcg attcgaacgt gaggtcccac
     2701 gaagcgtcga gccggtcggt gcgggcgctg cccctcgcgc gccccggggt gagcacggag
     2761 tccccgatgt ggatgtactc gccttcgctc gcgccgactt cacttgcggg aagcgtggtc
     2821 ttcgaagcgt gctgggcgtc ggcggtgaag agcacgaacc agagcgagcc cttcggctcg
     2881 gaacccggac gcttgtgcac cgtgtggcgg atccagaagc cgagcggctc ggtcgggtgc
     2941 gacgccttca ggtagaagct ctcgtagcgc cctgcctcgc gcggcacgtc cggaaaacgc
     3001 ggccgcgtgc gcaactctcc cgcgttcacg gcgcgcagcg tactcgccct gatacaaccc
     3061 gcgcgtggcg gacgagcgac ccgtgatcgt catcaccggc gcctcgagcg gaatcggggc
     3121 cgcgactgcg cggcgtgccg cggaggccgg gtggcgactc gtgctcgggg cgcgctcgga
     3181 ggaccggctc gccgcgctgg cgtccgagct cggaggcgac gaggttgcga tcggacgtcg
     3241 gtgcgatgtg accgactgga acgaccagca ggcgctggcg gcggccgcgc tcgatcactt
     3301 cgggcgcctg gacgcaatgt tcgcgaacgc cggct
//
LOCUS       DASVXS010000132         7243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1078901, whole genome shotgun sequence.
ACCESSION   DASVXS010000132 DASVXS010000000
VERSION     DASVXS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078901"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VF752_07240"
     CDS             <1..718
                     /locus_tag="VF752_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8855380.1"
                     /translation="LGAAHRLGLDRIVYVSSELALLPPSPGEVLTPDSPVKQPPWPYC
                     RSKADSERVARRYQALGAPVVSVLPAAVWGPHDPHFGEGATRATNILKRRYPIVMSGG
                     MQIADVRDLASVLAAVMTPGHGPRSYMVAGHYISLPDLIRTLADLTGRRIPFAIFPRW
                     FLAGFGRAADVVQRRVGTRLPWDGEGIWVMNCAARCDDSRTRGEFGLEPRPLRETLAD
                     TVRWLVEVGHLTPREAGRLA"
     gene            787..1449
                     /locus_tag="VF752_07245"
     CDS             787..1449
                     /locus_tag="VF752_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8855381.1"
                     /translation="MEAEHIYRHFCMTARALEVVGERWSLLIVRDLLLGPRRFTDLMR
                     SLAEITPSRLTSRLRRLEAAGVIVREPPERGREVWYRLTDAGLALGPTIDALTLWGIE
                     HARQQPVEDEPVPSESAMIGTKVWLSRFGPPPPDGLIWVWRFPGDDDFTLRAENGTWE
                     LSRGGDEGAAVAVDATRRAWATFLTSPRERRRLPTGEIGLEGSKREVRRFAKAFAAKP
                     AV"
     gene            complement(1453..3000)
                     /locus_tag="VF752_07250"
     CDS             complement(1453..3000)
                     /locus_tag="VF752_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855382.1"
                     /translation="MQSKPLILLSLLLAAFTINLDTTIVNVALPTLVRELHASTTQLE
                     WIVDAYNLMFAALVLACGNLGDRLGRKGVLLAGLGVFGAATIAGGLGTNPGQLIAARA
                     VMGLGAALIFPATLSLLTNVFTERGERARAIGLWGATTGVGIAVGPIVGGWLLERFSW
                     QSVFFALAPIAALAAVLVAARVPTSRDPQAPPADRAGLLLSTAGMAVLIYTIIEAPNR
                     GWGAGRSVGGFAIAAVLLVAFVAWERRAQAPMLDVGLFRNLRFSAASGSVTITFFSLF
                     GFIFLITLYFQFLKGYRPLSTGVRLLPVATLVGITSVLGTRLAVRSGTKLVVAAGLVS
                     LAAGLAWTSSASSSTSYLTIAGQMVLIGSGIGLTSAPATESIMGAVPRAKAGVGSAIN
                     DATRILGGTLGVAVIGSVYASLYSTHLTSALTVRLPEAAASGAHRSVGAAFGAAERLS
                     ATGHADLANALHQAATTAFFRGFETACLVAAAIAAAGAVFALAFIPAQPPSASAETAP
                     AVPATSP"
     gene            3479..5497
                     /locus_tag="VF752_07255"
     CDS             3479..5497
                     /locus_tag="VF752_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855383.1"
                     /translation="MSGAGFTRELLDWPPDVFALTNVLLRRAEVFRFAVSPPAGVRWP
                     PAGGGAGDWSDAVVDAGRRWGAWVEDQRGSVPDVLAEAWSVASERSDLPLEDLAEGRD
                     WRVCEALLTMHAIADEACAGLGSATAGANGRGAAYRARARELLATTGSLARFPPRLVR
                     VLPKIRTPPTGRASFARYACVEGSGLEAKWHKVPARHPGTNRLAEFVHFLLLPWPLRV
                     RESDFRPVEGSVQRLEREPFGLFEFVPSERLDMDLLDRVLTAARDEAGGVDVVVLPES
                     SVDASEIDELEAVLNRHGVIHLTAGVRQRSPRPGRLGDNWVHVGVNPRLGKGEGPSEG
                     PGQQWFHIRQNKHHRWSLDQSQIFQYHLGGTLHPDVQWWEAMEVPRQSLQFMTLGEEI
                     TVVSLVCEDLAENDQVAEVIRAVGPTGVMTVLLDGPQLASRWAARYASVLADDPGSTV
                     LTLTSFGMAQRSRPGDRDPSPVIALWKDPERGLREIPLEAGAQAVLLTACGSPATRRT
                     GDGRRPVDTGTHLYDVAVHQICASDGGSGSPSARTGASLPRVLDVHDVTVLTGWAEAV
                     AETLAHAPERIPAVLADAREGAAWRSALGIADPSPQLDKAIAFLSAVIERAAPEDGPP
                     SFDAVLAASRESGAGEQPLDELVRSALRATLEQLRSRQPRQPTASTLS"
     gene            5695..6603
                     /locus_tag="VF752_07260"
     CDS             5695..6603
                     /locus_tag="VF752_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8855384.1"
                     /translation="MGSRSPRCQIRSQVRESFRFDVTHAAVGLVDVFIRQGLYKDREG
                     LPQPPYVPGLEVAGTVRELGDGVDGFTVGEPVVTVSGTGPEGGYAAISVVDARFAVSL
                     GSSGIDLALAVATVPNAATAYLALTRVAHLQSGETVLVHGALGGLASTFPGIASSLGA
                     STIVGTVRPTSLPTAEASELPYNRLIGSDEFPDALIDERFDVVIDPVGGDVRTTSLGV
                     TGSARATAGRRQRQPRLAAQRRDKPPLGLQSRGPRLQRRFVSNRATPAHVVQQRHEPI
                     GGGPRNARNLQLPREIDRQRHVCWSR"
     gene            complement(6942..>7243)
                     /locus_tag="VF752_07265"
     CDS             complement(6942..>7243)
                     /locus_tag="VF752_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855385.1"
                     /translation="NGGERVFGINTESTPLVVAAVAASVVLASALLASDALVILAGVI
                     GFALVAAAFDVREAFHQADESRTNLIVIAAVVAALHLAIVASAATLARRRATRAP"
BASE COUNT         1123 a         2449 c         2562 g         1109 t
ORIGIN      
        1 gctgggtgcc gcgcaccgcc tcggcctcga ccgtatcgtc tacgtgtcaa gcgagctcgc
       61 gctcctgcct ccctcgcccg gcgaggtgct gactcccgac tcgcccgtca agcagccgcc
      121 ctggccctat tgccggtcga aggcggattc cgagcgggtg gcccgcaggt atcaggcgct
      181 cggcgcgccg gtcgtgagcg tcctgcccgc agcggtgtgg ggaccgcacg acccgcattt
      241 cggcgaaggg gccacgcgcg ccaccaacat cctgaagagg cgctatccga tcgtcatgtc
      301 gggcggaatg cagatcgccg acgttcgaga cctcgcgtca gtgctcgccg cggtgatgac
      361 cccgggacat ggcccgcgga gctacatggt cgccgggcac tacatctcgt tgcccgacct
      421 catccgcacg ctcgccgacc tcacgggacg acgcatcccc ttcgcgatct ttccgcgctg
      481 gttcctggcc ggcttcggcc gggccgccga cgtcgtgcag cggcgagtcg gcacgcgact
      541 gccttgggac ggcgagggga tttgggtaat gaactgcgcc gctcgctgcg acgactcgag
      601 gacgcggggc gagttcgggc tcgaaccgcg cccactccgg gagaccctcg ccgacaccgt
      661 ccggtggctc gtcgaggtcg gacacctcac gccgcgcgaa gcgggtcgcc tcgcctgagc
      721 tgccaggccc cgccacggtt ggctatcgag gcgatactct cgcctcggta gcgcatttgt
      781 aaagccgtgg aagccgagca catctaccgc cacttctgca tgaccgcccg tgccctcgaa
      841 gtcgtgggcg aacggtggtc gctgctgata gtccgtgacc tgttgctggg tccgcgccgc
      901 ttcaccgacc tcatgcgcag cctcgccgag atcacgcctt cccggctgac cagtcggctc
      961 cggcgcctgg aggcggcggg ggtcatcgtg cgcgagccac ccgagcgggg gagggaggtc
     1021 tggtaccggc tcaccgatgc cgggctcgcg ctcgggccga ccatcgacgc gctgacgctc
     1081 tggggcatcg agcacgcgcg tcagcagccg gtcgaggatg agcccgtccc ctcggagtcg
     1141 gcgatgatcg gcaccaaggt ctggctcagc cggttcgggc cgccaccacc cgacgggctc
     1201 atctgggtct ggcgttttcc cggcgacgac gacttcaccc tgcgggccga gaacggcaca
     1261 tgggagctgt cacgcggagg tgatgagggc gcggcggtcg cggtggacgc gacccgaagg
     1321 gcctgggcca ccttcctcac ctcgccgcgc gagcggcggc ggctgccgac cggggaaatc
     1381 ggactcgagg gctcgaagcg agaggtcagg agattcgcga aggcgttcgc ggccaagccc
     1441 gcggtctgac gatcatgggc ttgtcgccgg cacggccggc gccgtctccg cgctggccga
     1501 cggcggttgg gcggggatga acgccagggc gaagacggcc ccggcggctg cgatcgcggc
     1561 ggcgaccagg caggcggtct cgaagccccg gaagaaggcc gtggtggcgg cctggtggag
     1621 ggcgttcgcg aggtcggcgt gtcccgtggc actgaggcgc tcggctgcgc cgaaggccgc
     1681 gcccacggat cgatgcgctc cgctggccgc ggcttcgggc agccgcacgg tgagcgcgct
     1741 cgtcaggtgg gtcgagtaca gcgacgcgta cacactcccg atgaccgcca cccccagggt
     1801 tcccccgagg atccgggtgg cgtcgttgat cgccgagccc acgccggcct tggcgcgagg
     1861 gaccgcgccc atgatcgact cggtggcggg tgcgctcgtg aggccgatgc ccgagccgat
     1921 gagcaccatc tggcccgcga tcgtcaagta gctcgtactc gaggacgccg acgacgtcca
     1981 ggcgagaccg gcggcgagcg agaccaggcc cgccgcgact acgagcttgg taccgctgcg
     2041 cacggcgagc ctggtcccga ggactgacgt gatcccgacc aacgtagcga cgggcaacag
     2101 gcgcaccccg gtggacagcg gtcggtagcc cttgaggaac tggaagtaga gcgtgatgag
     2161 gaagatgaac ccgaacaggg agaaaaacgt gatcgtcacc gacccgctcg ctgcgctgaa
     2221 tcgcaggttg cggaacagcc ccacgtcgag catcggcgct tgcgcgcggc gctcccaggc
     2281 cacgaaggcc acgagcagaa cggcagcgat ggcaaagcca ccgacgctcc gcccggcgcc
     2341 ccagccacgg ttgggcgcct cgatgatcgt gtagatcagc acggccatgc ccgccgtgga
     2401 gagaagcagg ccggcgcgat cggcgggcgg ggcctgcgga tcacgggagg tcggcaccct
     2461 cgcggccacg agcaccgccg ccagcgccgc gatcggcgcc agtgcgaaga agacgctctg
     2521 ccacgagaaa cgctcgagca gccagccgcc gacgatcggg ccgactgcga tcccgacccc
     2581 cgttgtcgcg ccccacagcc cgatcgccct ggcacgctcg ccgcgttcgg tgaacacgtt
     2641 cgtcagcagc gagagcgtgg ccgggaagat gagggcggct cccagcccca tcacggcgcg
     2701 cgcggcgatc agctgaccgg ggttggtgcc gagaccgccg gcgatggtgg cggcgccgaa
     2761 cacgcccagc ccggcgagca gaacgccctt gcgtcccagg cggtcgccga ggttgccaca
     2821 ggcgagcacg agggcggcga acatgaggtt gtaggcgtcg acgatccact cgagctgcgt
     2881 tgtcgacgcg tgcagctcgc gcaccagcgt cggcagcgcc acgttcacga tcgtcgtatc
     2941 gaggttgatc gtgaaggccg ccagcagcag cgagagcagg atcagcggct tactttgcat
     3001 gtggcgtcgg cgtgcgtccc tcgcggaaca attctcggac tcgcttacta tcgctttgat
     3061 agtaccgtgc cagtacaatt ctcgacaaga ggacccgctg tcacgacgcg ctggctcctg
     3121 ctggtcgtcc gaggcggcgc gccgtgtcac ttcgaggcaa gagcagcggc gcgcggcgcg
     3181 gccgggcgac tgtggcgacg gacgacttcc gatagtgccg ctcggcatgg ataggagcag
     3241 cggcgccggg cgctttactc ggtgccgctt tcaggcgaaa gtagcgatgg tatgtcggcc
     3301 gtctacaccc tcgctggcac gcggaagacg cagtccgcgc tcatcgatgg ctgaaacgcg
     3361 agatccggct acgcctccac cgtccgatca ccggcacgta tcgcgaatga ctgcagtcgt
     3421 gggggattcc tcgtccacgc ggccgcatca agagacgctg gcttcggctt ggcgggctgt
     3481 gtctggagcc ggcttcacgc gcgagttgct cgactggccg cccgacgtgt tcgctctcac
     3541 gaacgtgctc ctccgtcggg cggaggtgtt tcgatttgcg gtctcgccac cggcgggggt
     3601 gcggtggccg ccggccggcg ggggggctgg tgactggtcc gatgccgtgg tggacgcggg
     3661 tcggcgctgg ggtgcatggg tcgaggatca acggggttct gtccccgatg tgctcgccga
     3721 ggcctggagt gtcgcgtcgg agcgatccga tctgccgctc gaggatctcg cggaggggcg
     3781 cgattggcgg gtctgcgagg cgctgttgac gatgcacgcg atcgctgacg aggcgtgcgc
     3841 cgggcttggc agcgcgaccg cgggtgcgaa cgggcgcgga gccgcgtatc gcgctcgggc
     3901 gcgggagttg ctcgccacga cggggtcgct ggctcggttc ccgccgcggc tcgtgcgggt
     3961 gctgccgaag atccgtacgc cgccgaccgg ccgggcgtcg ttcgcacgct acgcatgcgt
     4021 ggagggatcg ggactcgaag ccaagtggca caaggtgccg gcccgtcacc ccggcaccaa
     4081 ccgactggcg gagttcgtcc atttcctgtt gctgccgtgg ccgctgcggg tccgcgagtc
     4141 cgacttccgt cccgtggagg gatcggtcca acggctggag cgcgagccgt tcggactctt
     4201 cgaattcgtc ccatccgaac ggctcgatat ggatctcctg gatcgcgtgc tgaccgcggc
     4261 tcgggacgag gccggcggcg tcgatgtcgt cgtgttgccc gagagctccg tcgatgcgag
     4321 cgagatcgac gagctcgagg ctgtcctgaa ccgccatggc gtgatccatt tgacggctgg
     4381 ggtccgtcag cgctcaccgc gcccggggcg gctcggggac aactgggtgc atgtgggcgt
     4441 gaacccgagg ctcgggaagg gcgaggggcc gtccgagggc cctggccagc agtggtttca
     4501 catccgccag aacaagcacc atcggtggtc gctcgaccag agccagatct tccaatatca
     4561 cctcgggggc accctgcacc cagacgtcca gtggtgggag gcgatggaag ttccacggca
     4621 gtcgttgcag ttcatgacac tcggcgagga gatcacggtt gtctcgctcg tgtgcgagga
     4681 cctcgcggaa aacgaccagg tcgccgaggt catccgcgcg gtcggcccga ccggcgtcat
     4741 gacggtcctg ctcgacggac cgcagctggc atctcgctgg gcggcccgct acgcgagtgt
     4801 gctggccgac gacccagggt cgaccgtcct caccctgacc tcgttcggga tggcgcagcg
     4861 cagtcggccg ggagaccgcg acccctcccc agtcatcgcg ctctggaagg accccgagcg
     4921 aggcctccgc gagatcccgc tcgaggcggg cgcccaggcg gttctactca cagcgtgcgg
     4981 ctcgccagcg acccggcgca ccggcgatgg ccgccgcccc gtcgacaccg ggacgcatct
     5041 ctacgacgtc gcggtgcatc agatctgcgc ctcggacggc ggctcgggat ccccgtcggc
     5101 caggaccggg gcgagcttgc cccgggtgct cgacgtccat gacgtcacgg tcctgaccgg
     5161 gtgggcggag gcggtggcag agacccttgc ccacgccccc gagcgcatcc ccgccgtgct
     5221 ggccgacgcg cgcgagggcg ctgcctggcg atcggcgctt ggcatcgcag acccgtcccc
     5281 gcaactcgac aaggcgatcg cattcctcag cgctgtgatc gagagggccg cgcccgaaga
     5341 cggcccaccc tcgttcgacg ccgtgctcgc cgcctcgcgc gagagcggag cgggcgagca
     5401 gccgctcgac gagcttgtcc gcagcgccct ccgcgcaacc ctcgaacaac tccgttcgcg
     5461 ccagccgcgg cagccgacag cgtcaacgct gtcctaatcg aggagcgcga tctcgacgcg
     5521 ttcgcggatc cagtcccgtg cgcggtcctc gccccacccg aagtcatcgg cggcgagcgc
     5581 gaactgctcg aaccggaagt gctagtagat gatccgagcg gcgtcctcgt cggacaggtc
     5641 cttcgtgaga tttgaccatg cgagcggcaa tcgtcacacg ctttggagcc cccgatgggt
     5701 tcgaggtctc cgagatgcca gatccgatcc caggtccggg agagctttcg attcgatgtc
     5761 acgcacgctg ccgttggact cgttgacgtc ttcattcgcc aagggcttta caaggaccgt
     5821 gagggcctgc ctcaaccgcc atacgtgccg gggctcgagg tcgcgggcac ggtccgtgag
     5881 cttggtgacg gggtcgacgg cttcaccgtt ggcgagccgg tggtcaccgt gtcggggacc
     5941 ggtcccgaag gcggctacgc ggcgatcagc gtggtcgacg cccgcttcgc cgtcagcctt
     6001 ggtagctcag gcatcgacct cgccctggcc gtcgcgacgg tgcccaacgc cgcgaccgcg
     6061 tacctcgcgt tgacacgggt cgcgcacctc caatcgggcg agactgtgct cgtgcacggc
     6121 gccctcgggg ggctggcgtc cacctttccc gggatcgcca gcagcctcgg ggcctcgacg
     6181 atagtcggca ccgtgcgccc caccagtctc ccgaccgctg aggcttcgga actgccttat
     6241 aaccggctga tcggcagcga tgaatttccc gacgcgctga tcgatgaacg attcgacgtg
     6301 gtgatcgatc cggtgggcgg cgatgtgcga acgaccagcc tgggcgtcac tggctccgct
     6361 cgggcgactg ctggccgtcg gcaacgccag ccgcgattgg cggcacagcg tagagacaaa
     6421 ccgcctctgg gtctccaatc tcgcggtcct cggcttcagc gtaggttcgt ttcgaaccgc
     6481 gcgacacccg ctcacgtcgt ccaacagaga cacgaaccga ttggcggcgg tccccgcaac
     6541 gcgcggaatc tccaacttcc gcgtgagatt gatcgtcagc gtcatgtctg ttggtcgcgc
     6601 taggccgtga cgatcgccac ggcgattagc gcgatccaag tgcttccgag gaggatgggg
     6661 atcccgtggg cgagccacca cgaggccgac cacttgggaa gaatcgagag cgggaacgac
     6721 aacgaaagaa gaagtgcgcc cagggagact cgaactccca cccgggatac cccggacaag
     6781 gccctcaacc ttgctagcgg cgcgctcgta gcgtccgcgg gggtccagat cgtccggtct
     6841 agagcgaaat ctcggaaggt ctggacgatt cgagacgatg tggatgttgc cacggtgttg
     6901 ccaccgttgg accgtcgcga cggccctcgc caggcccgac ttcaaggtgc tcgggtggcg
     6961 cgccggcggg cgagcgtggc ggcgctggcc acgatcgcga ggtggagcgc cgcgacgacc
     7021 gcggcgatga cgatcaggtt cgtgcgggac tcgtccgctt ggtggaaggc ctcgcggacg
     7081 tcgaaggcgg cggcgacgag cgcgaagccg atgacaccgg ccaggatcac gagcgcgtcg
     7141 cttgcgagca gcgcggacgc aagaacgaca gaggcggcca ccgcggcgac gaccagcggc
     7201 gtcgactcgg tgttgatccc gaacacgcgc tcgccgccgt tct
//
LOCUS       DASVXS010000133         3445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_318104, whole genome shotgun sequence.
ACCESSION   DASVXS010000133 DASVXS010000000
VERSION     DASVXS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318104"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..767)
                     /locus_tag="VF752_07270"
     CDS             complement(105..767)
                     /locus_tag="VF752_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855386.1"
                     /translation="MNQRTPMRVVVGEDQPLVREGIVRVLEDAGFEVVGTAGDAPDLV
                     RKARAHRPDVVVTDIQMPPDGTDDGLRAALEIRESDPDVGVLVLSQFLESRYALDLVG
                     EHAEGVGYLLKEKVGEIRTFTDAVERVARGGSVLDPEVVASLVGRKRHESPLDELTNR
                     ERQVLSLMAEGRSNAAIAGELVVTVAAVERHVTSIFGKLGLHQSPDQHRRVLAVLRYL
                     QD"
     gene            complement(798..1034)
                     /locus_tag="VF752_07275"
     CDS             complement(798..1034)
                     /locus_tag="VF752_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855387.1"
                     /translation="MVPHTIVCEPRLVVYKIYNGFWSFGRPTVEELRHDLRAVLMKHH
                     WDSDRSEAQVRAAWERGERDRFYPPELGWPAPTA"
     gene            1593..>3445
                     /locus_tag="VF752_07280"
     CDS             1593..>3445
                     /locus_tag="VF752_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8855388.1"
                     /translation="MFSRITAFVSRHPWRVILVWLALGALATAYAQSRQSDVITNDTS
                     SFLPNKYESVRATKLGEREFGVAKGATRVTALVKRADGAPLTSGDRRQIAALSAAMTH
                     WRPNWSAVHPVGSDQKNVRPSAHQRETSVVSAQTSPPRDRGRFELVSLQFKGNDSDPV
                     IQGAFFQFRADTQKQFRAHRLSAGFTSGVATSADTGKANASTASLEQTLLLAAIVLLN
                     LLFFRGVLAAIVPLMAVVLVAGTAGGLIVLSAKLFGYHFDINTPQLITTVLIGIGTDY
                     YLFMAFRFRERLRAGDQRKPAAALAGGQVAGVIASAALAVAAAFATLGLAQFGQFRVL
                     GPAVAISVLVMLVAGVTLFPAVLAATGPKMFWPSKSWRRTLENGLATRIGRLVARRPA
                     LVATSTIALLAVLAAGAIGVRMNYDLGNGAPHTEATRVAEEINRSLPRGVTDPQHVYV
                     TSASRLVPGELQRMRTALARVGGVGQVSQPVISRDGHAADIGVALKYDSTTKRAMNTA
                     SGPLRQAAHGSAPQGAEAVVGGTATIYSDIADSINHDLKLIFPLAAVLILIILVALLR
                     SAVAPVYLLVAVALEFAATLGAAALLFQHGLGQPGVAFTMPLVLFLFVVALG"
BASE COUNT          531 a         1210 c         1159 g          545 t
ORIGIN      
        1 tcggaccgcc aacgagagga ggccgcgtgc cgctatttcg atgagcgccg gcagtggccg
       61 gacgagacct gacgggcacg ccggccgagc ccccatgccg gtcgtcagtc ctggaggtag
      121 cggagcacgg cgagcacgcg ccggtgctga tccggcgatt gatggagccc gagcttgccg
      181 aagatgctgg tcacgtggcg ctcgaccgcg gcgaccgtga ccacgagctc cccggcgatc
      241 gccgcgttgg agcgcccctc ggccatcagc gagagcacct ggcgctcgcg gttcgtcagc
      301 tcatcgaggg ggctctcgtg ccgcttgcgg ccgacgagcg acgccaccac ctcgggatcg
      361 agcaccgaac cgccacgggc gacacgctca acggcgtcgg tgaatgttct gatctctccg
      421 accttctcct tgagcaggta gcccaccccc tcagcgtgct cgccaacgag gtcgagggcg
      481 taccgggact ccaggaattg ggacagcacc agcacgccga cgtcgggatc ggactccctg
      541 atctcgagcg ccgcgcgcaa gccgtcgtca gttccgtcag ggggcatctg gatgtcggtg
      601 acgacaacgt cggggcggtg cgcgcgcgcc ttccgcacga gatcgggagc gtcccccgca
      661 gtgcctacca cctcgaatcc ggcgtcctcg agcacgcgca cgatcccctc gcgaacgagc
      721 ggctgatcct cgccgaccac cactcgcatc ggggttcgct ggttcacgag ccaaggctat
      781 gggatcggcc gcgggcgtca ggcagtcggc gcgggccagc cgagctcggg cgggtagaag
      841 cgatcccgct cgccgcgctc ccaggccgct cggacttggg cctctgagcg gtccgagtcc
      901 cagtggtgct tcatgagcac cgcacgcagg tcgtggcgca gctcctcgac tgtgggacgg
      961 ccgaaggacc agaagccgtt gtagatcttg tagacgacca ggcggggctc gcagacaatc
     1021 gtgtgcggca ccatctgtcg tgcagcgggt tgttgtactc cgctatgtcg aggtcctgct
     1081 gcacgatcct gcccgggtcg gcaaggaacg gccactcggc gccgacgccg ttgcggtagt
     1141 tgagggcctc ctgcggatcg gtcgtggtga tggtcactat gcggcagtag cggacgccgg
     1201 gcttcatctc cctccacagc tgcacgagcc cctcgtgctg aacctgggtc cttcgccgag
     1261 taggcctcgc gcgccagcac gatgaccatc ggatcggcgt gctggatctc ggacagcctg
     1321 tgcggctctc ccgtgtggtc cggcaattcg tagtcgggaa acacgcgtcc cactttcacg
     1381 tcggctcgca tggcagcgct cctgaggcag ctgggagccc acgctacgcc cgcatcgggc
     1441 gggcttccaa gcgggcgctc ccgagccgtg tgtggggaat cccctaagcg tcgttggtgg
     1501 gtccgcccaa gcaccggggt tgaggccgct caatgggcgg ccggcgccgc gcgcgcgacg
     1561 ctcagggcac caacccagag agggggaaca ccatgttcag ccgaatcaca gccttcgtaa
     1621 gtcgtcaccc ctggcgcgtg atcctcgtct ggctggcgct cggtgccctc gccacggcgt
     1681 acgcacagtc gaggcagtcc gacgtgatca cgaacgacac ctcgtcgttc ctgcctaaca
     1741 agtacgaatc ggtgcgagcc accaagctcg gcgagcgcga gttcggcgtc gcgaagggcg
     1801 cgaccagagt caccgcgctc gtcaaacgcg ccgacggcgc gcctctcacc agcggcgacc
     1861 gccggcagat cgccgccctc agcgcggcga tgacgcactg gcggccgaac tggtcagccg
     1921 tgcacccggt cggctctgat cagaagaacg tgaggcccag cgctcaccag cgcgagacca
     1981 gcgtggtgtc cgctcagacc tcgcccccgc gggaccgtgg ccgcttcgag ctcgtctccc
     2041 tgcagttcaa gggaaacgac tcggacccgg tgatccaggg agcgtttttc cagttccgcg
     2101 ctgacacgca aaagcagttc cgcgcccacc gactgagcgc cggattcacg agcggcgtgg
     2161 ccacctcggc tgacaccggc aaggccaacg cgtccacggc gagcctcgag caaacgctgc
     2221 tgctcgcagc gatcgtgctg ctcaacctgc tgttcttccg cggcgtgctc gccgcgatcg
     2281 tgccgctgat ggcggtggtg ctggtcgccg gaacggccgg cggtctcatc gtgctgtcgg
     2341 cgaaactgtt cggctaccac ttcgacatca acacgccgca gctgatcacg acggtcctga
     2401 tcggcatcgg caccgactac tacctgttca tggccttccg cttccgcgag cggctccgcg
     2461 ccggcgacca gcgcaaacca gcagccgcgc tcgccggggg acaggtggca ggggtgatcg
     2521 cctccgctgc gctggcggtg gcggccgcgt tcgccacgct cgggctcgcc caattcggcc
     2581 agttccgggt gctcggcccc gccgtcgcaa tatcggtgct ggtcatgctc gtggccggcg
     2641 tgaccctgtt cccggcggtg ctggccgcga cgggtccgaa gatgttctgg ccgtccaagt
     2701 cctggcgccg cacgctcgag aacggtctgg ccacacgcat cgggcgcctc gtcgcgcgac
     2761 gccctgcgct cgtcgcgact tccacgatcg cgttgctggc ggtgctggcc gcgggcgcga
     2821 tcggagtgag gatgaactac gacctcggca acggcgcccc gcacaccgag gcgacacgcg
     2881 tagcggaaga gatcaacagg tcactgccgc gcggcgtgac ggacccgcag cacgtctacg
     2941 tcacgagcgc atcgcggctc gtgccaggcg agctacagcg aatgcggacc gcgctcgcga
     3001 gggtgggcgg cgtcggccag gtctcgcagc ctgtcatctc gcgcgacggc cacgcggccg
     3061 acatcggggt cgcgctcaag tacgactcga cgaccaaacg ggcgatgaac acggccagcg
     3121 gaccgctgcg ccaagctgcc cacggcagcg ccccgcaggg cgccgaggcg gtggtcggtg
     3181 gcaccgcgac gatctactcg gacatcgcgg actcgatcaa ccacgacctg aagctgatct
     3241 tcccgcttgc ggccgtgctg atcctgatca tcctggtcgc cctgctgagg agcgctgtgg
     3301 cgccagtcta cctgctcgtg gcggtggcgc tcgaattcgc agcaaccctc ggagccgcgg
     3361 cactgctctt ccagcacggg ctcgggcagc cgggcgtcgc gttcaccatg ccgctcgtcc
     3421 tgttcctgtt cgtggtcgcg ctggg
//
LOCUS       DASVXS010000134         9665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1169030, whole genome shotgun sequence.
ACCESSION   DASVXS010000134 DASVXS010000000
VERSION     DASVXS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169030"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF752_07285"
     CDS             <1..281
                     /locus_tag="VF752_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855389.1"
                     /translation="PTASPHGPGALSQPRTTTGSTGPVPVAGAPEPVVPTPSLPNAPS
                     LPKVTVPPPSNLLPPPTGLSQPGASPVDQSVQGVNDTAGATVGGLVGR"
     gene            413..634
                     /locus_tag="VF752_07290"
     CDS             413..634
                     /locus_tag="VF752_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855390.1"
                     /translation="MPATEPRTWRGEPGGWLEGLDFLTYGAAAAGLGLAALIVALLVL
                     DSLALAIVGGAILLAVAGAYLANDFFNDW"
     gene            complement(621..1331)
                     /locus_tag="VF752_07295"
     CDS             complement(621..1331)
                     /locus_tag="VF752_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8855391.1"
                     /translation="MKHPVPAPPTELSAHDGLAYALFLPDTTPSAGVVVLHGAGSAKE
                     NHFDFARAARGFGLAALAFDARGHGESEGEFGPGCIADVMTMCTLLREHVARVAVRGS
                     SMGGFKAIFAAAESHGEIGAVIAICPAPPSQLLRGIRSGSLSSFRMDRSALEEWLPKL
                     DIEAAAASLAPETALLLMHAEGDESVPYTVSQEIYAAAGRPKQLLLLPGGHHRSIQHD
                     DELQAESLRFIEKRLTSR"
     gene            1348..2631
                     /gene="lysA"
                     /locus_tag="VF752_07300"
     CDS             1348..2631
                     /gene="lysA"
                     /locus_tag="VF752_07300"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX8855392.1"
                     /translation="MAPPLSDQASHAAPGPGAGSALPSQVWPRGTRINEGGRIEIGGC
                     DLVEVAAEFGTPAYVYAEDDMRSRAREFLESFRSRTDHFEVTYASKAFPATAVYRLMG
                     EEGLSIDVASGGELSLALRGLVDPARIYMHGNNKTEAELAYAVESGVGHIVVDSFDEI
                     ERLERIAPGQKILLRVTPGIKPSTHSHIQTGQEDSKFGFGLDDVPRAIEALRTLELRG
                     LHAHIGSQVFDLDPYEKLAEVLTQLGDYPLLNLGGGLGIAYTRDEIPPSIDEYVEALL
                     RRAPEGVTVLCEPGRALVGNAGVTIYTVGTRKEVPGIRTYIAVDGGMSDNLRPALYGA
                     RYEAEIASRPGGSEIVTIAGMHCETGDILASDVPLDSPRPGDVLAMPATGAYGHAMAN
                     NYNAVPRPPVIFCRNGDARVVVRRETYEDLALRDV"
     gene            2621..3835
                     /locus_tag="VF752_07305"
     CDS             2621..3835
                     /locus_tag="VF752_07305"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX8855393.1"
                     /translation="MTFNVGLLGHGTVGAAFHELFESRAEAIEASTGMRPEISGVLTR
                     SRGDFEDLLERSDLIVEVMGGVEPAREYMLRAMQAGKHVVTANKQVLSHHGEELYAAA
                     RESGVQLRFEAAVGGVVPVIRVIHETLAAAHIERVHGIVNGTTNFILTEMARTGASYE
                     EALADAQRLGYAEADPSEDVNGKDAAAKMAILARLAFGAPVHIEDVPAEGIEQLTSDD
                     IAYAKEFGLSLKLIGTAERVDGGISVRVFPAFLYGDHPLASVNGSFNAVTIESASITE
                     ITLSGPGAGGPQTASAVLGDVISAMIPPPTLMAPAVEHRIVKDVESAFYLHLEVADRP
                     GVLAQIAEVLGLQGVSVKSVIQHGLGEEARLVMVMHPVLESSFRAAVDLIARLDFMRE
                     PPRVIRVIEETF"
     gene            3832..4830
                     /gene="thrC"
                     /locus_tag="VF752_07310"
     CDS             3832..4830
                     /gene="thrC"
                     /locus_tag="VF752_07310"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8855394.1"
                     /translation="MSLWRYRDRLPVEPLVTLGEGDTPLVQAPHLSERVGAEVWLKFE
                     GANPTGSFKDRGMTVAVSAAVAEGAQAVVCASTGNTAASAAAYAAKAGLKGAVIVPEG
                     KIATGKLAQALMHGARVVALRGNFDQALELVRELAERQPISLVNSVNEFRIEGQKTGA
                     FEICDELGEPPDVLCIPVGNAGNITAWWRGFQEYGVAPRLHGYQAEGAAPLVRGEPVE
                     HPETVASAIRIGNPARWEDAMNAVTASRGEIRAVSDEEILGAYELLGRREGIFCEPAS
                     AASVAGLLKYGAEGRVVCVLTGHGLKDPQTALRQAAPVIPCEPEIEAVEQAVLGGP"
     gene            4827..5678
                     /gene="thrB"
                     /locus_tag="VF752_07315"
     CDS             4827..5678
                     /gene="thrB"
                     /locus_tag="VF752_07315"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX8855395.1"
                     /translation="MNRRRLVRVPASSANLGPGYDVLAAAMSIHLELEVEEGGGFSVE
                     TNVPGVSPDRDNLCVQAFELLHPADDISFRIRSEIPLAAGLGSSAAAIVAGLAAADHM
                     YELDADLYRLATDLEGHPDNVAAALHGGFVVCTRDGVERLDPIAELEGIIAIPPERVL
                     TNEARGALPVDVPLSDAVHNVAHVALLMIGLSRADLDLVGRGLADRLHQPRREHLYPR
                     SMNLVRAARDLGAVGATISGAGPSVLFWTHFEQTGQLMERLREVAADCELRRVQFAPE
                     GANVRAL"
     gene            5835..6569
                     /locus_tag="VF752_07320"
     CDS             5835..6569
                     /locus_tag="VF752_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855396.1"
                     /translation="MRIPRTRGGLTGLALMILGAWAALIPFVGPYFNYQMHNDQTWFW
                     FADRMWLEILPGAAIFVGGMVLTFSAFRASARSGALLAALGGIWLVIGPSMSMLWHSG
                     RIEVGPALGGSGTRTLEWIGFFYGAGALAIALGSYAYGMLTTRPVVGTAEAAAAGTAG
                     GVAARERAYAREPVATRTGAGTGAATGAAAGAATGAAAGRTVGKRRRGGLFGRFRRDR
                     DGDGVPDNQETRVVNREDDTVTTTRR"
     gene            complement(6745..7389)
                     /locus_tag="VF752_07325"
     CDS             complement(6745..7389)
                     /locus_tag="VF752_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8855397.1"
                     /translation="MGTARLSRKESQARTRELLRDAAKHLFAERGLEGASIDDIAHEA
                     GFTKGAFYANFDSKHALYLELLDEYFADRLDEVEDALRHEGSPEDQARRAGLGFASHI
                     GADSEWRRLYIEFCAHALRDERFRHELAIRLRGLRAEITRLYTRRAADLPYPPPFPLE
                     QITTMTGLMVDGFALEKVLDPEALPDDVSGTMLYVFFTGLRTLAEQSAQANDWQ"
     gene            complement(7389..8744)
                     /locus_tag="VF752_07330"
     CDS             complement(7389..8744)
                     /locus_tag="VF752_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8855398.1"
                     /translation="MSAQTKTMPPGPKWPAPIATLGWWTRPNPLVRRMRDRYGETFSF
                     PILHEGRWVFISDPEDIKTVFTAPAKAAHAGEANAILKPILGRNSVLLLDEAPHLRQR
                     KLLLPPLHGERMQSYGELMTQIAQDEIDSWPAGEPMRLWPRMQAITLEVIMRAVFGIR
                     DAARLEELRGMLAQILHWTSSAQQMLLLTVFGPEKATEAPHVKRALAPIDAVLIDEIR
                     RRRGEPDLEEREDILSMLVQARHEDGSPMSDTELRDELMTLLVAGHETTATALSWAGE
                     RLVRHPDKLERLRAEVEAGNEDYLDAVVKETLRIRPVLPIVLRLLKEPMTLGGYELQA
                     GTYVAPSIYLVHHREDIYPEPDRFMPERFLEKPAGTYTWFPFGGGIRRCLGAAFALFE
                     MKAVLRALVSSVELRAVEPQNELTTRRVIVHTPARGASVVVRRLVRSDSGERESVAAA
                     A"
     gene            complement(8811..>9665)
                     /locus_tag="VF752_07335"
     CDS             complement(8811..>9665)
                     /locus_tag="VF752_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8855399.1"
                     /translation="ISRGPRIGDAFLSTDGMLCRTVEDTARTLDVLAGYEPGDASWAP
                     PPSEPFADAMLREPSGLRIALVAEPPMPSSIDDVSMGAARSAAALLESLGHSVEEVTP
                     PWHGSRDLLPAFTAIFGTAIASGVLYGARSTGLEPNEETLEPLTLALFRRVNEMTAPD
                     YAVAFGFIQGHARRIVEWALGYDFLITPALGQRPVQIGEIDACSDDPLDDFRRSGVFT
                     PFTAVFNTTGQPAMAFPLGVADDGLPTSVQIVGRPAGEAALLALAAQLEAARGPFEER
                     PPLVAEPA"
BASE COUNT         1425 a         3294 c         3467 g         1479 t
ORIGIN      
        1 ggccgacggc cagcccccat ggaccgggcg cgcttagcca gccgcgcacc acgacgggct
       61 cgaccggtcc cgtccccgtt gcgggagccc cggagccggt ggttccgacg ccgtcgctcc
      121 cgaacgcgcc gtcactcccc aaggtcacgg ttccgccacc gtccaacctc ctgccgccgc
      181 cgaccggcct cagtcagcca ggtgcctcgc cagtcgatca gtccgttcag ggcgtgaacg
      241 acacggcggg cgcgaccgtc ggcggactgg tcggccgcta gcgcaaagcg ccgcctgcgg
      301 ttaagttcgc tacgtggcga gcgatcggac cgtctgcccg cactgcggca ggctgagccg
      361 gacgatctgg ggcgcgtgtt ccgagtgcgg acgcaccagg gaagctgcag cgttgcccgc
      421 aactgagccg cgcacatggc gcggcgagcc tggcgggtgg ctcgagggcc tcgatttctt
      481 gacgtacggc gccgccgcgg cggggctcgg actggcggcg ctcatcgtcg cgctccttgt
      541 tctcgactcg ctcgcgctcg cgatcgtcgg cggcgcaatc ctgctcgcgg tcgcgggtgc
      601 ctacctcgcg aacgacttct tcaacgactg gtgagccgct tctcgatgaa gcgcagcgac
      661 tcagcctgca gctcgtcgtc gtgctggatc gagcggtggt gtccgccggg caacagcagc
      721 agctgctttg gccgcccggc cgcggcgtag atctcctgcg acacggtgta gggcaccgac
      781 tcgtcgccct ccgcgtgcat gagcagcagc gctgtttcgg gggcgagtga ggccgccgcg
      841 gcctcgatgt cgagcttcgg cagccactcc tcgagcgccg accggtccat cctgaagctc
      901 gagagcgagc ccgagcggat gccgcgcagc agttgcgacg gcggtgccgg acagatggcg
      961 attaccgcgc cgatctcccc gtgtgactcg gccgccgcga agatcgcctt gaagccgccc
     1021 atgctcgatc cgcgcacagc cacccgcgcg acgtgctcgc gaagcagtgt gcacatcgtc
     1081 atcacgtcgg cgatgcaacc cgggccgaat tcgccctccg actcgccgtg gccgcgcgcg
     1141 tcgaaggcga gtgccgcgag cccgaagccg cgggcggcgc gcgcgaagtc gaagtggttc
     1201 tccttcgcgg agcccgcgcc gtgaagcacc acgacgcccg cgctcggcgt ggtgtcgggg
     1261 aggaacagcg catacgcgag tccgtcgtgc gccgatagct ccgtgggcgg ggctgggacc
     1321 gggtgcttca taatcgccgc tccgattatg gccccaccac tgagtgatca ggcgtcgcac
     1381 gcagctcccg gacccggggc cggctcggcg ctgccgtcgc aggtgtggcc gcggggcacc
     1441 cggatcaacg agggcggccg catcgagatc ggcggctgtg acctcgtcga agtggccgcc
     1501 gagttcggca ctccggccta tgtgtacgcc gaagacgaca tgcgctcgcg cgcgcgtgag
     1561 ttcctcgagt cgttccgctc gcgcacggat cacttcgagg tgacgtacgc gtcgaaggca
     1621 ttcccggcaa ccgccgtcta ccgcctgatg ggcgaggaag gcctgtcgat cgacgtcgcc
     1681 tcgggcggtg agctgtcgct tgctctgcgc ggcctcgtgg accccgcgcg catctacatg
     1741 cacggcaaca acaagaccga ggccgagctc gcctacgcgg tcgagtccgg tgtgggccac
     1801 atcgtggtcg attcgttcga cgagatcgag cgcctcgagc gaatcgctcc cggccagaag
     1861 atcctgctgc gggtcacgcc gggcatcaag ccgtccacgc acagccacat ccagacgggc
     1921 caggaggact cgaagttcgg cttcggcctc gacgacgtgc cgcgcgcgat cgaggcgttg
     1981 cggacgctcg agctgcgcgg gttgcatgcg catatcggct cgcaggtgtt cgacctcgat
     2041 ccctacgaga agctcgccga ggtgctcaca cagctcggcg actacccgct gctgaatctc
     2101 ggcggcgggc tcggcatcgc gtacacgcgc gatgagatcc cgccctcgat cgacgagtac
     2161 gtggaagcgc tgctccgccg agcgcccgag ggcgtgacgg ttctctgcga gcccgggcgc
     2221 gcgctggtgg gcaacgccgg cgtgacgatc tacacggtcg gcacgcgcaa ggaagtgccc
     2281 gggatccgca cctacatcgc cgtggacggc ggaatgtccg acaacctccg gccggcactg
     2341 tacggcgcgc gctacgaggc cgagatcgcg agccgccccg gcgggtccga gatcgtgacg
     2401 atcgcaggca tgcattgcga gaccggcgac atcttggcga gcgacgtgcc gctcgactcg
     2461 ccgcgtcccg gcgacgtgct ggcaatgccc gcgacgggcg catacggtca cgcgatggcg
     2521 aacaactaca acgcggtccc gcgaccgccg gtgatcttct gcaggaacgg cgacgcccgc
     2581 gtggtggtgc gccgcgagac ctacgaggac ctggccctcc gtgacgttta acgtcggcct
     2641 cctcggccac ggcacggtcg gggcggcgtt ccatgagctg ttcgagtcgc gggccgaagc
     2701 gatcgaggcc tccaccggga tgcggccgga gatctcgggc gtgctcacgc gcagccgcgg
     2761 ggacttcgag gacctgctcg agcgcagcga cctgatcgtg gaggtgatgg gcggcgtcga
     2821 gcccgcgcgc gagtacatgc tgcgcgcgat gcaggccggc aagcacgtgg tgacggcgaa
     2881 caagcaggtg ctgtcccacc acggcgagga gctgtacgca gcggcgcgcg agtccggcgt
     2941 gcagctgcga ttcgaggccg cggtcggcgg ggtcgtgccc gtgatccgcg tgatccacga
     3001 gacgctcgcc gccgcgcaca tcgagcgcgt gcacgggatc gtgaacggca ccaccaactt
     3061 catcctcacc gagatggcgc gcaccggggc gtcgtacgaa gaggcgctcg ctgacgcgca
     3121 gcgcctgggc tacgcggagg cggatcccag cgaggacgtg aacggcaagg acgcggccgc
     3181 aaagatggcc atccttgctc ggctcgcctt cggcgctccc gtgcacatcg aggacgtgcc
     3241 cgctgagggc atcgagcagc tcacgagcga cgacatcgcg tacgccaagg agttcgggct
     3301 gtcgctgaag ctgatcggca cggccgagcg cgtggacggc ggcatctccg tgcgcgtgtt
     3361 ccccgcgttc ctgtacggcg accatccgct cgcgtcggtg aacggctcgt tcaacgccgt
     3421 gacgatcgag tccgcttcga tcaccgagat cacgctgtcg gggccgggcg ccggcggccc
     3481 ccagacggca tccgcggtcc tcggcgacgt gatcagcgcg atgatcccgc cgcccacgct
     3541 gatggcgccg gcggtggagc accggatcgt gaaggacgtg gagtccgcgt tctacctcca
     3601 cctcgaggtg gcggaccggc cgggcgtgct cgctcagatc gcggaggtgc tcggcctcca
     3661 gggcgtgtcg gtgaagtcgg tgatccagca cggattgggg gaggaggcgc ggctcgtgat
     3721 ggtcatgcac ccggtgctcg agtcgagctt ccgcgcggcg gtcgacctga tcgcccggct
     3781 cgacttcatg cgcgagccgc cccgcgtgat ccgagtgatc gaggagacct tctgagtctc
     3841 tggcgctacc gcgaccggct ccccgtcgag ccgctcgtca cgctcggcga gggcgacact
     3901 ccgctcgtgc aggcgccgca cctctccgag cgcgtgggcg ccgaggtgtg gctgaagttc
     3961 gagggcgcga acccgaccgg ctcgttcaag gatcgcggca tgaccgtcgc cgtgtccgcg
     4021 gcggtggccg agggcgcaca ggcggtggtg tgcgcctcga ccggaaacac cgccgcgagc
     4081 gccgccgcgt acgccgcgaa agccgggttg aagggcgcgg tgatcgtgcc ggagggcaag
     4141 atcgccaccg gcaagctcgc gcaggcgctg atgcacggcg cgcgcgtcgt tgcgctgcgc
     4201 ggcaacttcg accaggcgct ggagctggtg cgcgagctgg ccgagcgcca gccgatctcg
     4261 ctggtgaact cggtgaacga gttccgtatc gagggccaga agacgggcgc gttcgagatc
     4321 tgcgacgagc tcggcgagcc gcccgatgtc ctttgcatcc ccgtcggcaa cgcaggcaac
     4381 atcacggcgt ggtggcgcgg cttccaggag tacggcgtcg cgccgcggct gcacggctat
     4441 caggcggagg gcgccgcgcc gctggttcgg ggcgagccgg tcgagcatcc ggagacggtt
     4501 gcctccgcga tccggatcgg caacccggct cgctgggagg acgcgatgaa cgcggtgacc
     4561 gcctcgcgcg gtgagatacg cgccgtgagc gacgaggaga tcctcggggc ctatgagctg
     4621 ctcggccgcc gcgagggcat cttctgcgag ccggcgtcgg cggcatcggt cgcggggctg
     4681 ctcaagtacg gcgccgaggg gcgcgtggtg tgcgtgctca ccgggcacgg cctgaaggac
     4741 ccgcagaccg cgcttcgcca ggccgcgccc gtgattccct gcgagcctga gatcgaggcg
     4801 gtcgagcagg ccgtgctcgg agggccgtga accgcaggcg gctcgttcga gtgcccgcgt
     4861 cctcggccaa cctcggcccg ggctacgacg tgctggccgc ggcgatgtcg attcacctgg
     4921 agctcgaggt ggaggagggc ggcggcttct cggtcgagac gaatgtgccc ggcgtctcgc
     4981 cagaccgaga caacctctgc gtgcaggcgt tcgagcttct ccatccggct gacgacatca
     5041 gcttccggat ccggtcggag atcccgctcg ccgccggcct cggctcgagc gcagcggcga
     5101 tcgtggctgg gctcgccgca gccgaccaca tgtacgagct cgacgccgac ctctatcgcc
     5161 tagccactga tctggagggg cacccggaca acgtcgcggc cgcgctccac ggcggcttcg
     5221 tggtgtgcac gcgcgacggg gtcgagcgac tcgacccgat cgccgagctc gaggggatca
     5281 tcgcgatccc tccggagcgc gtgctgacca acgaggcgcg cggagcgttg ccggtcgacg
     5341 tgccgctgag cgacgccgtc cacaacgtgg cgcatgtggc gctgctgatg atcgggctct
     5401 cgcgtgcaga cctcgatctg gtcggaaggg gcttggccga ccggctgcac cagccgcgtc
     5461 gcgagcatct gtacccgcgc tcgatgaacc tggtgcgcgc cgcccgtgat ctgggcgccg
     5521 tgggcgcgac catttcgggc gcggggccga gcgtgctgtt ctggacccac ttcgagcaga
     5581 ccggccagct gatggagcgg cttcgcgagg tggcggccga ctgcgagctc cggcgcgttc
     5641 agttcgcccc agagggcgcg aacgtgcgcg ccctgtagcg ggacgcggac gagccaggct
     5701 gcgctggcac acgagccgag gcctgtctcg tccgcgtcgc cgcaaaaggt cgtgcgtttt
     5761 cgcacttttg gggttaatcg ggggccccgg gtgggaaatc tcccggagag gcaattaggg
     5821 aggaaccaag gacgatgcga attccacgga cgcggggcgg cctgacgggc ctcgcattga
     5881 tgattctcgg cgcatgggcg gcgctgatcc cattcgtcgg gccgtatttc aactaccaga
     5941 tgcacaacga ccagacgtgg ttctggttcg ctgaccgtat gtggctcgag atcctgcctg
     6001 gagccgcgat cttcgtcggc ggcatggtgc tgaccttcag cgcgttccgc gcgtctgcgc
     6061 gcagcggtgc gctgctggca gcgctaggcg gcatctggct ggtgatcggg ccctcgatga
     6121 gcatgctctg gcatagcggc aggatcgagg tcgggccggc gcttggcggc agcggcacgc
     6181 gaacgctcga gtggatcggc ttcttctacg gcgccggtgc gctggcgatc gcgctcggct
     6241 cgtacgcgta cggcatgctc acgacgcggc cggtggtcgg caccgccgag gctgcagcgg
     6301 ccggtacggc cggaggtgtc gcggcgcgcg agcgcgcgta cgcgcgtgag ccggttgcga
     6361 cgcggacggg tgcgggcacc ggagcggcga cgggcgccgc ggctggtgcc gcaaccggag
     6421 ccgcggcggg acgcaccgtc ggcaagcgcc gtcgcggtgg gctgttcggc cgcttccgcc
     6481 gcgatcgtga cggcgacggg gtgcccgaca accaggagac cagggtcgtg aaccgtgaag
     6541 acgatacggt cacgaccaca cgccgctaga cggcgaactc ttccgagggg ggaagacaca
     6601 ggggtcggcg agcgggacgc cggccctttg tgcttctgga gggttcgcgg gcaacggagg
     6661 aggaccaagc gaagtcccaa aacttcgagt cccgcacgcg ggaacaccgc ttcccgacgt
     6721 tttgcgacct gctcttggcg gccgttattg ccagtcgttc gcctgtgccg actgctcggc
     6781 cagggtccgc aggccagtga agaagacgta gagcatcgtg cccgacacgt cgtccggcag
     6841 cgcttccgga tcgagcacct tctcgagcgc gaagccgtcc accatcaggc cggtcatggt
     6901 cgtgatctgc tcgagcggga acggcggcgg gtacggcaga tcggcggccc gtctcgtata
     6961 cagccgcgtg atctctgcgc gcagcccgcg cagccggatc gccagctcgt ggcggaaccg
     7021 ctcgtcgcgc agcgcatgcg cgcagaactc gatgtagagc cgccgccact ccgagtctgc
     7081 cccgatgtgg ctcgcgaagc caagcccggc gcggcgcgcc tgatcctcgg gtgaaccctc
     7141 gtgtcggagc gcgtcctcga cctcgtcgag ccggtcggcg aagtactcgt cgagcagctc
     7201 caggtacagc gcgtgcttgc tgtcgaagtt cgcgtagaag gcgcccttgg tgaagccggc
     7261 ctcgtgggcg atgtcgtcga tcgacgctcc ctccaggcca cgctcggcga acaggtgctt
     7321 cgctgcatcg cggagcagct cgcgtgtgcg cgcctggctc tccttgcgcg atagccgtgc
     7381 ggtgcccatc aggccgctgc cgccaccgac tcccgctcgc cggagtcgct ccgtacgagc
     7441 cgtcgcacga ccacactggc tccgcgcgcc ggcgtgtgca cgatcacgcg acgggtggtg
     7501 agctcgttct gcggttcgac ggcgcggagc tccaccgagc tcaccagcgc gcgaaggacg
     7561 gccttcatct cgaacagcgc gaacgccgcg cccaggcaac ggcgaatgcc accaccgaac
     7621 gggaaccagg tgtaggtgcc ggccggcttc tcgaggaagc gctcgggcat gaagcggtcg
     7681 ggctcgggat agatgtcctc gcggtggtgc acgaggtaga tcgacggcgc cacgtacgtg
     7741 cccgcctgaa gctcgtagcc tccgagcgtc atcggctcct tcagcaggcg cagcacgatc
     7801 ggcagaaccg gccggatgcg gagcgtctcc ttcacgaccg catcgaggta gtcctcgttg
     7861 ccggcctcca cctcggcgcg gaggcgctcg agcttgtctg gatggcgcac gagccgctcg
     7921 cccgcccagg agagcgccgt cgcggtcgtc tcgtggccgg cgacgagcag cgtcatcagc
     7981 tcgtcgcgca gctccgtgtc ggacatcggg ctgccgtcct cgtgcctcgc ctggacgagc
     8041 atcgagagga tgtcctcgcg ctcctcgagg tcgggctcgc cgcggcggcg ccggatctcg
     8101 tcgatcagca cggcgtcgat cggcgcgagc gcgcgcttca cgtggggcgc ttcggtcgcc
     8161 ttctccgggc cgaacacggt caggagcagc atctgctgcg ccgacgacgt ccagtggagg
     8221 atctgcgcaa gcatcccgcg cagctcttcg aggcgcgcgg cgtcgcggat gccgaagaca
     8281 gcgcgcatga tcacttcgag ggtgatcgcc tgcatccgcg gccacagccg catcggctcg
     8341 cccgccggcc agctgtcgat ctcatcctgc gcgatctgcg tcatgagctc gccgtagctc
     8401 tgcatgcgct cgccgtggag gggaggcagg agcagcttgc gctggcgcag gtgcggcgcc
     8461 tcgtcgagca gcagcaccga gttgcggccc aggatcggct tgaggatggc gttcgcctcg
     8521 ccggcgtgag ccgccttggc gggcgcggtg aagacggtct tgatgtcctc ggggtccgag
     8581 atgaacaccc atcgtccctc gtgcaggatc gggaacgaga aggtctcgcc gtacctgtcg
     8641 cgcatgcgcc tcacgagcgg attcgggcgc gtccaccagc cgagcgtggc gatcggggcc
     8701 ggccacttcg gtcccggcgg catcgtcttg gtctgggcgc tcatcggacc ctcctcggca
     8761 tactcgtcgg tattcgaata ccgaaaggta tctgatttgg gccgagtgcg ctatgccggc
     8821 tcggcgacga gcggcgggcg ctcctcgaac ggtccgcggg ccgcctcgag ctgtgccgcg
     8881 agtgctaaca gcgctgcctc gcccgccggc cggcccacga tctgcaccga tgtggggagg
     8941 ccgtcgtcgg ccacgccgag cggaaacgcc atcgccggct ggccggtggt gttgaagacg
     9001 gccgtgaagg gcgtgaagac gcccgagcgc ctgaagtcgt cgagcgggtc gtcgctgcac
     9061 gcatcgatct cgccgatttg caccggccgc tgaccgagcg caggcgtgat caggaagtcg
     9121 tatccgagcg cccactccac gatcctgcgc gcgtgccctt gaatgaagcc gaaggccacg
     9181 gcgtagtccg gcgcggtcat ctcgttcacc cggcggaaca gcgcgagcgt gagcggctcg
     9241 agcgtctcct cattgggctc tagccctgtc gaccttgcgc cgtacagcac gcccgaggcg
     9301 atcgccgtgc cgaagatcgc ggtgaacgcc ggcagaagat cgcggctgcc gtgccacggc
     9361 ggcgtcacct cctctactga gtggccgagc gactcgagca gcgccgccgc agagcgggcc
     9421 gctcccatgc tcacgtcgtc gatcgagctc ggcatggggg gctccgcgac gagcgcaatc
     9481 cgcaggccgc tcggctcccg cagcatcgca tccgcgaacg gctcggaagg cggcggagcc
     9541 cagctggcgt cgcccggctc gtagcccgcg agtacatcga gcgttcgcgc ggtgtcctcc
     9601 acggtgcggc agagcatgcc gtcggtcgac agaaaagcgt cgccgatgcg aggcccgcgc
     9661 gagat
//
LOCUS       DASVXS010000135        22833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_391403, whole genome shotgun sequence.
ACCESSION   DASVXS010000135 DASVXS010000000
VERSION     DASVXS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22833
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391403"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VF752_07340"
     CDS             complement(<1..718)
                     /locus_tag="VF752_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8855400.1"
                     /translation="MRLEGRTALVTGGAGGIGAATCRRLAAEGAQVAVTDMNTDAAGT
                     VAAEVNGMAYELDVRSTASIAAAVKAAEDELGPIDVLVNNAGYDEFGFFVNTTEDQWD
                     RVLNVNLRGVIAVSHALVPGMQERRRGRIVNVASEAGRVGSHGSSIYSAAKAGVIGFT
                     KTVARESARYGITCNAVAPGPIETPLLMQAPEQLGDLGERIIQGMVGQTALRRLGQPD
                     EVAALITFLGSDDASYITGQA"
     gene            759..1448
                     /gene="npdG"
                     /locus_tag="VF752_07345"
     CDS             759..1448
                     /gene="npdG"
                     /locus_tag="VF752_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX8855401.1"
                     /translation="MAPNKPIPIIGGTGALGFGLALRFAREGLPVVIGSRDQGRADEA
                     AGRVREQVPDAQVEGLENTEAAQRGPIVFLTVPFRAQSEYLTNLKPVLQPGQILVDAT
                     VPLAAAVSGRATRLLGVPQGSAAQQAQEMAPDGVEVVAALHSVSAPLLRDLDHELDED
                     IPVAGDRQEAKRRVAELIERIPGLRPVNAGRLEVARLIEGITPMLISVNGRYKTHAGL
                     KLTGLPDELWQ"
     gene            1439..2347
                     /gene="cofD"
                     /locus_tag="VF752_07350"
     CDS             1439..2347
                     /gene="cofD"
                     /locus_tag="VF752_07350"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX8855402.1"
                     /translation="MAVAVLAGGSGGAKLARGMLDVVGPEGLAVIANTGDDVEIYGAH
                     VSPDPDLVTWWLADVIDERGWGIEGDTFNVMDALREAGREVWFNLGDRDLALCIVRSE
                     ALARGERLTEAHARVCRAVGVNVRVLPMSDNPVRTFVRVHGEWRAFQEFMIQERGSEP
                     EDVDLRALERAQPTPEVLDAIAGSDAVVIGPSNPIVSIGPILEVPGMRDAIEASQAPV
                     VAVSPFVGGHAVKGPTEAFCRMAGLPVGPLAVVEAYEGLVDGLVSDEPVPDAGMPVLQ
                     IPTLMDTPDARRELAQRTLDFARALA"
     gene            2407..3090
                     /gene="cofC"
                     /locus_tag="VF752_07355"
     CDS             2407..3090
                     /gene="cofC"
                     /locus_tag="VF752_07355"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX8855403.1"
                     /translation="MRTAAILPVKSFAAAKQRLSPTLGSGSRQALALAMVSDVLGALR
                     RVDGVEEVFVVTAERTVEAAARGGRVRVLHDPAETGQSSATGIGIDRAAAEGFERVVL
                     IPGDTPLLDPRELDVLLARAEEQSLDVAIVPDRHGAGTNALVLRPPHAITPSFGPGSL
                     KRHVTAAEQASLRFEVVEIPSLMHDVDDPADLHDLSAALDERRGHAPLTQGALRQLGR
                     MRAAPPVEV"
     gene            3105..3842
                     /gene="cofE"
                     /locus_tag="VF752_07360"
     CDS             3105..3842
                     /gene="cofE"
                     /locus_tag="VF752_07360"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX8855404.1"
                     /translation="MLALAPLPEVRPGDDLAALLAEASGEGLREGDVLVVASKVVSKA
                     EGRIARLRDVEPSPRAREIAAENDKDARLVQLVLDESSELLRAERGVLITETRQGYVC
                     ANAGIDQSNVPGDDSVVLLPLDPDNSARALREDLGRTHGVRPAVVITDSFGRPWRLGQ
                     IDVAVGAAGLRVLDDWRGRNDREGRELAVTEIAVVDAVAAAADLARAKDAGEPGVLVR
                     GLERFVTEEHGPGAAALRRPREQDLFR"
     gene            complement(3903..4472)
                     /locus_tag="VF752_07365"
     CDS             complement(3903..4472)
                     /locus_tag="VF752_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855405.1"
                     /translation="MDARSVERFDREVTVRSRQEFSDAAAALLIGALAGLAFTFASAL
                     GFALAFGSVVALGLALVAGLKRHFLIERLTLDRDAYAIEAVRSRGRRLTSRRARKRLS
                     QSLRTLPADASQRTSCVPPDRVAAFAADIDEIARELEQPETDVDPVSAAACLRLLTHG
                     ADSPLLNPDLPIEDLRAALMRIRGGIHTR"
     gene            4542..5336
                     /locus_tag="VF752_07370"
     CDS             4542..5336
                     /locus_tag="VF752_07370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8855406.1"
                     /translation="MRLGLPIAAIAVLVAIAMLLTEGLTSSDQEPSRTVVQTPPATKA
                     PSGKPPVQAEGVNTTQPAKPAWRGPRHLTALVPRRTQLRDRPHGRPIAALPARTTWKS
                     PRVLFVAKRRDGWLGVVAPELPNGHIGWIPHSIARIYPVSWELRADLSARRVTLMHAG
                     RVVRRVPVAIGGPGTPTPRGRFAVTDKLEVKAASPYGCCILALSGHQPNIAQGWGGGD
                     RIALHATTNEATVGSAVSHGCLRARARDMKVLIREVPLGTPIYIKA"
     gene            complement(5431..7620)
                     /gene="cofH"
                     /locus_tag="VF752_07375"
     CDS             complement(5431..7620)
                     /gene="cofH"
                     /locus_tag="VF752_07375"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX8855407.1"
                     /translation="MRRVTFSRNFTLSLSRTCQCYCKYCAFATHRPHLYTADEVEKML
                     DDAARRGVKELLVLTGEKPEVNPEVAARLGELGHADFTSYVAWACERALERGMLPHTN
                     IGVASRDELARLREVTASQGLMLESVNPDLVVHQGSPTKHPARRLDMIRAAGELRIPF
                     TTGILVGIGEEPDERIAALEAIAAVQAEFGHIQEVILQNFVPHPRYYGQEPAEIADEA
                     QRSQTHGEALPLPDWAKPISLDDMRLLVRESKRLMPDVGVQIPPNLSDWWLPLVEEGA
                     TDLGGLSANGDHISPEHPFPSVHRMRKQLAPRGYALTERLCVYPQYMTPEWLEQGVLD
                     VIKLKYWSFIPRRGSGRREERAIRQDTVPGAIERARDGEWLNEEELTALFAENRPEAL
                     EDMRQAADELRAELAGDTVTFVVNRNINVSNVCIVGCAFCGFGQGRRSPDAYEHSRAE
                     FVRRVEEAVEFGATEICMQSGIHPDWSIEDYEKWLRTAKEVAPDIHLHAYSPMEVAHI
                     CDVSGLPPLEVFARLRDAGLDSTPGTAAEVLHDGVRERISPNKLPVRRWVEIIEASHA
                     AGLRSTVTVMFGHVEEPWELAEHMRVVRELQERTGGFTEFVPLSFIPFHTLLGRTHGI
                     EEISRDDNLKHTAAFRLALGKTIPSLQASWVKMGLDVATEALRWGVNDLGGTLMEESI
                     SRMAGSYHGVRLEPADLIGAAHAAGRPAAERTTLYEIRRRFEVPAAA"
     gene            8762..9832
                     /locus_tag="VF752_07380"
     CDS             8762..9832
                     /locus_tag="VF752_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855408.1"
                     /translation="MAKLDTPPGAIGALVLGPQAFIKGFDIGSGLNRKYFRLGLPAKG
                     VGSPASPALRFLTQGTRVGTGANMVMPKDGWVLSWCLGCGSTEFLVFPDGSASNGDCI
                     SAYQQPPHGLSALMGDYSACNTHTGPYPGLVVDMQYAAFWTDEYGSEAFDWYPMEDYT
                     GQPGTTHIPDPSSLSQSTADANVAGELRNNPDEYSILIPWLRWTTGEPNACDPTNPLV
                     CNPDDEQNRRECELSDHLTELDNPTADPYDVHDDFTRLDWDGDTIPTALKYGTDSWGY
                     RKIAPTHGWGPPDVLQTQAALNGPPDDPTFKGSGTEVYDGATYTGTDGSICFRRVIVR
                     HTANLGEVAPKEIISSYGRKVP"
     gene            9833..10120
                     /locus_tag="VF752_07385"
     CDS             9833..10120
                     /locus_tag="VF752_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855409.1"
                     /translation="MADEDRKHELACLAATTAVLDGAKRLLGRKVVSSVRLEGAKPET
                     EIVVQWRWDYEQEPHEQRFKFWSADFAESDGTPGRPDEVAAVILANVEAEQ"
     gene            complement(10154..11479)
                     /locus_tag="VF752_07390"
     CDS             complement(10154..11479)
                     /locus_tag="VF752_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8855410.1"
                     /translation="MPALVDELIDWLRIPSVSSGGGDADAIRAAGQWVVDRIAAAGGS
                     AELVETDGNPLAVGELRASTDPESAPTILIYGHYDVQGVGPLELWESGPFDADMRDGK
                     VYARGAVDDKGNFLPLLHVAADMARAGELPVNVRVLAEGEEEVGSREVMRWIARDERG
                     ADAAIVFDSGMLDARTPAITVAVRGMVAVAIDVRVASADLHSGLYGGAVLNAVHVLHE
                     VLSVVLPGPDGVLREELQEGVASPSPEELAGWRSLPDAAAAIAESGGRPLGPHAVADF
                     YARTWAGPSIDVNGIEGGEAEQERTIVPATARAKLSMRLAPGQEYSEMAPVLERLLRE
                     AVPEGADVDIRMHGSDPALFDPTLPAIQLGSDAIERATGVKPALVRSGGSIPILAAFA
                     ERRIPTILSGFMLPEDALHAPNESFGLDRLEMGARSARELYLALAAMRA"
     gene            complement(11491..12264)
                     /locus_tag="VF752_07395"
     CDS             complement(11491..12264)
                     /locus_tag="VF752_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8855411.1"
                     /translation="MSDRIRLDQRVALVTGAGSPEGIGFATARILAERGARVAIVSTT
                     ERIHERALEVGDAAAGFVCDLRYPDAVDQMLNDVERRLGEIDVLVNNAGMTTVNEEMK
                     SKPFTELGEGAFERELAITLLTTYYVTRGVTPGMVQRRYGRIVNVSSVTGSHVSYPAQ
                     APYSAAKAGVDGLTRALAVDLGPSGITVNSVAPGWIATASLSPMERRAGQHTPLGRAG
                     TPDEVGELIAWLASDAASYVTGQSIVIDGGNTVQEVKGG"
     gene            complement(12261..13607)
                     /locus_tag="VF752_07400"
     CDS             complement(12261..13607)
                     /locus_tag="VF752_07400"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8855412.1"
                     /translation="MDRGRLAQLLAAERERFAADHPRSRELFERSKRSLVAGVPMSWM
                     TMWAGGFPLFFEEARGARITDADGREYVDLCLGDTGAMAGHAPEAVAEVVSARARRGV
                     TAMLPTEDAAWVGEELRRRFGLPRWQFALTATDANRWVLRMARAITARPKVLVFNYCY
                     HGTVDETFISLDERGRPRSRHGNVGPAVDPVLTSEVVEWNDPEALERALDGGEIACVL
                     AEPALTNIGIVLPEPGFHDALRQLTRDAGTLLLIDETHTMSAGPGGYTAAHGLEPDLL
                     TVGKAIGGGVPVAAYGMSEELEQRLLTLPGVDLEDVGGVGGTLAGNALSLAAARATLE
                     HVLTEEAFERMTALAERFAAGVNDVIATYSLPWHVVRLGARAEYRYRPEPPRNGGEAA
                     AASDPELDAFMHVFLMNRGVLITPFHNMALMCPATTEADVDAHTEAFAAAAETLAA"
     gene            complement(13668..14417)
                     /locus_tag="VF752_07405"
     CDS             complement(13668..14417)
                     /locus_tag="VF752_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX8855413.1"
                     /translation="MEERGVAAYIGEFIGTFALVFFITAVVSLFVTPPTPGAATQPFI
                     DWSVVGLVHIFVLFMLIQTLAVISGAHFNPAVTLAMAAIRQIKPADAVVYWAMQLAGG
                     TLGALLTKALLLNEGRDVNWGAVGVSNLLDGKAGLGFVCELIGTFFLMWAIVGVAVNP
                     AGLKDWSGFVIGGTLGFLVMIFGSLTGAGFNPARAFGPALVASDFGGAGKFLLVYVAG
                     PLVGATLAALTYVYLFVTPGRREVGAMEPVG"
     gene            complement(14493..18512)
                     /locus_tag="VF752_07410"
     CDS             complement(14493..18512)
                     /locus_tag="VF752_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855414.1"
                     /translation="MRRVAGVLACGLLALAAYTPSALAAFPYPSHTAPGQVPNDLDSG
                     TEWKYAATSEPGNLAVNSDPTELNGVRGAHIVDKDPSVDTAWQHTTGRPDVVIGELDS
                     GVKWNDRGAMLDLRHKIRLNKGELPVPNHDRTTPLEEGADCSSYKSAYDASGDGVFNV
                     TDYACDSRVERDPAARAAKGQPRGQGPGDLLDPQDVLIAFSDGRDQDRNGFTDDIAGW
                     DFLDNDNDPFDDVQYGHGTGELRDSTAEADNGDDLGTCPNCMSVPLRVGDSFIADSND
                     FAQAVLYAVDNNMLVVQEALGTLNNSKLARDAVEYAYDHGVAVIASAADESAQHHNWP
                     STYPHVIVVNSVRKYDDTFTAQPRSYLQFNGCTNFSSKITVSIPSTSCSSEATGRGAG
                     IAGLIYSAALDAHDKGRLGTNPDCKRVNGDSCVISANEVRQLMAMRSDDVAFTAQPEP
                     SCSSPAGGGAPFCTDPNFAPRLPQHQAVLPLPGSRRYTARKGPDPYYGYGRINAAKSV
                     GVFDATVKLPPEAELYSPEWYAQIDPRTASTPVTGFVGARGNSYRCQVYVAPGSYPND
                     GTAPDGDFKPVGGGYCGDSTHTASFKGVLAKIDVADLKGRFPANAGDFQGREPGTTPQ
                     TSNGRPSNDAYGFTVKVVVTSEQGGTKLTGDDRRNMYLHRDSDLLPGFPRQLTGDGAS
                     SPLFADLDGDNRNELVFGSSDGRVHAMRRDGSELPGFPVLTDALPLHTGERAFTSGDV
                     STSASRTAMLSSVAVSDLNGDGSPEIVGSDMGGKVYVWSAKGLVWKREANPDWSGKPL
                     SPFAPERYDAQNPDESQRHRTQHGFIASPVLADLDGDGKLEVVQAGMDRHMYAWHADG
                     SQVKGFPVLVVDPAKVDSVDPRSHVVHFKSSAGPALNQGAIVDTPALADLNADHRPEI
                     VVGTNEEYDGAGDGGPNVSEFNAASYGALATVLGPANSRIYAIKPTGDPDGNAQTVGD
                     AFVDGWPFKVALLQKEILPVVGEGITGPPSTGPVNCPSGGSGTKVGVIPDGGPAYILN
                     GDGTSCYGNGPDGKPIALSDTGQGVDETDRPIVPGLGMSAFGKLDGSSASFLAPAIGL
                     MRTLDIAAPEYQGGQDYVGAWNPATGQFQPGWPGHTNDLQFLTGPSIADIDGMPGEES
                     VSASAYEDVQAYDFAGQPVNSRWPKLNSDWTVANPLIGSFGTLDTSDGAHKVVVNETR
                     AGGVFAWGVDAPSCSPGSWPRFHHDNASSGDFNRDAVVPGRPYDGSKSGSSAVRFRAP
                     GDDLLCGKAARYEVVQSSKPVDEASFGSAQKVDGAPEPAEAGTLQTLTLPAAHARYIA
                     VRAVDEQGNAGRPLMIDTR"
     gene            complement(18588..19334)
                     /locus_tag="VF752_07415"
     CDS             complement(18588..19334)
                     /locus_tag="VF752_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8855415.1"
                     /translation="MKNAIVTGASSGIGEATARLLAQRGYRLIAVARRAERLDQLKAE
                     LGDAVIPLSLDLTDGDAPAHVREAVDREFGGQLDLLVNNAGGAWRAAFGDDEKGGWEN
                     VRRTMELNFDAVVRLTEALLPVLRASAPSAIVNVSSVAARASRPKSGAYSAAKAALAS
                     WTDALHLEEKPNNVHVGMVLPGFIVTEGFPQKDIKPRWIVSKPEKAAEAIFKAGPGGK
                     PEVIVPRGYALIPALKTFAPGLVRRGASGV"
     gene            complement(19331..19660)
                     /locus_tag="VF752_07420"
     CDS             complement(19331..19660)
                     /locus_tag="VF752_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855416.1"
                     /translation="MARVALLCPDLLFGSKLEGGLRAAGHEVARFDRPEDASAAEADV
                     LVVDLNAPEFDGSAARAPDGAPRLGFYSHVDQDTRRRAEEAGFELVVPRSRMAREMTA
                     LVDRLAA"
     gene            complement(19663..20025)
                     /locus_tag="VF752_07425"
     CDS             complement(19663..20025)
                     /locus_tag="VF752_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855417.1"
                     /translation="MALADDFQQIVDSLPPDWTNLVCDLRIFDEDRYIEAATILVQIN
                     AQPYSKHDWHWRLPVAHEFGHAAPAETVRGTLALLDEQGIEGELAARDARSGRVEITQ
                     GWGRPESVRREFARRRSQ"
     gene            20063..20983
                     /locus_tag="VF752_07430"
     CDS             20063..20983
                     /locus_tag="VF752_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX8855418.1"
                     /translation="MTVATEYELLRGSAGLVDRPERGKLLLTGAEAAEFLQGQLTNDI
                     ESLAPGTGCYAALLTHKGKLLADMRVLRGEDWILIDSEAAAMPAVAKNVRMYSIGRDV
                     QARDVSTERAILSLIGPTSRDALDVAPPDAEHSFVEGEHGLYVTTDLGVDVICDAADA
                     DGVRAALGIDEVSPDAAELLRVESGRPRLGVDMDGDTIPQEAGLNERAVSFTKGCYVG
                     QETVARLHYKGKPNRHLRGLRLSAPASHGDPIVLGDREVGRIGSVADSPRLGPIALAI
                     VRREASPGDTVLVGEGRAAAELAELPFTAG"
     gene            21109..21312
                     /locus_tag="VF752_07435"
     CDS             21109..21312
                     /locus_tag="VF752_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855419.1"
                     /translation="MTTLTQSQVNRKLAELDLLAQEAWLDYRDGLRELDGREYEDAED
                     QSWLRLQERLQELDRERQLLVAS"
     gene            21246..22241
                     /locus_tag="VF752_07440"
     CDS             21246..22241
                     /locus_tag="VF752_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8855420.1"
                     /translation="MATTPREAPGARQGAPAARGKLSADSPLLGGPLAHDPREDVYEL
                     PQPPAPEREPGTDLRQLRRLLPATEPSRAVDDWGRSERIVSLMEPVLDFYYDYWFRVE
                     VEGIENVPAEGGALLVSNHSGALPPDAPMIIWAIRREHPAPRPVYMLGEHWFKGYPGV
                     GMLANKIGLVAAHPANAQRLLRDEGRLVLVFPEGQKGSRKLYWQRYRLRRFGRGGYVK
                     TALRSGVPIVPIAVIGAEEAMPIFAHVPLLQRLTGLIYFPVNHAFPHFGLAAAGMYLP
                     AKFKIRFLSPISLDDYGPEDADDLQLVQSLSEHVRACIQREVDALVAARKSVWFG"
     gene            22264..22710
                     /locus_tag="VF752_07445"
     CDS             22264..22710
                     /locus_tag="VF752_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855421.1"
                     /translation="MRATERFSRGTRVAVAASAVAVFAAGCGGGSDFQNKPRPAVPVQ
                     LTGVITRDKVTVSPDSVGAGPVIIVVSNQTPEKHTLTLDGGGGTQQVRVGPIAPTETG
                     RITGDLAPGSYQVKAGTEQAQVREIAPATLHIGKARKSSSDTLLLP"
BASE COUNT         3449 a         8037 c         7872 g         3475 t
ORIGIN      
        1 tcgcctggcc cgtgatgtag ctcgcgtcgt ccgagccgag gaaggtgatc aacgccgcga
       61 cctcgtccgg ttgcccgagc cgccggagcg cggtctgccc gaccatgccc tggatgatgc
      121 gctcgccgag gtcgcccagc tgctcgggcg cctgcatcag cagcggcgtc tcgatcggcc
      181 cgggcgccac cgcgttgcac gtgatcccgt agcgcgccga ctcgcgcgcg accgtcttgg
      241 tgaacccgat cacgcccgcc ttcgcagccg agtagatgct cgagccgtgc gagcccacgc
      301 ggcccgcctc tgaggccacg ttcacgatcc gcccgcggcg gcgctcctgc atgcccggca
      361 cgagcgcgtg gctgaccgcg atcacgccgc gcaggttcac gttcagcacg cggtcccact
      421 ggtcctcggt ggtgttgacg aagaagccga actcgtcgta gccggcgttg ttcactagga
      481 cgtcgatcgg cccaagctcg tcctcggccg ccttcaccgc ggcggcgatc gaggccgtcg
      541 agcgcacatc gagctcgtac gccattccgt tcacctcggc cgcgaccgtc ccggcggcgt
      601 ccgtgttcat gtccgtcacg gccacctgcg caccctcggc tgcgagccgg cggcaggtcg
      661 cagccccgat tcctcccgcg ccgccggtca cgagcgcggt ccttccctcg agtctcatgg
      721 ccgggcagcc taaccgcgtc cggtaggttg caccgcccat ggcgcccaac aaacccatac
      781 cgatcatcgg cgggacggga gcgctgggct tcggcctggc gttgcgcttt gcgcgtgaag
      841 ggctcccggt cgtgatcggc tcgcgcgacc agggacgcgc cgacgaggcg gccgggcgtg
      901 tccgcgagca ggttccggac gctcaggtcg aggggctcga gaacactgag gccgcgcagc
      961 gcggcccgat cgtgttcctc acggttccgt tccgcgcgca gtccgagtac ctcaccaacc
     1021 tcaagccggt gctacagccg ggccagatcc tcgtcgacgc caccgtgccg ctcgcggcgg
     1081 cggtctccgg ccgggccaca cggctgctcg gggtgccgca ggggtcggcc gcccagcagg
     1141 cgcaggagat ggcgcccgac ggcgtagagg tcgtggccgc cctgcattcc gtgagcgccc
     1201 cgctcttgcg cgacctcgat cacgagctcg acgaggacat ccccgtggcg ggcgaccgtc
     1261 aggaggcgaa gcggcgcgtg gccgagctga tcgaacgcat ccccggactg cggccggtga
     1321 acgccgggcg cctagaggtc gcacggctga tcgaggggat caccccgatg ctgatctccg
     1381 tgaacggccg ctacaagacg cacgcggggc tgaagctcac cgggctgccg gacgagctgt
     1441 ggcagtagcg gttctcgccg gcggcagcgg aggcgcgaag ctcgcgcgag ggatgctcga
     1501 cgtggtgggg cccgaggggc ttgccgtgat cgccaatacg ggcgacgacg tggagatcta
     1561 cggcgcgcac gtgtcgcccg accccgacct cgtgacctgg tggttggccg acgtcatcga
     1621 cgagcgcggc tggggcatcg agggcgacac cttcaacgtg atggacgcgc tgagggaggc
     1681 cggccgcgag gtgtggttca acctcggcga cagagacctg gcgctctgca tcgttcgcag
     1741 cgaggcgctc gcccgaggcg agcggctcac cgaggcgcac gcgcgggtgt gccgcgcggt
     1801 aggcgtgaac gtgcgcgtgc tgccgatgag cgacaacccg gttcgcacgt tcgtgcgcgt
     1861 gcatggcgag tggcgtgcgt tccaggaatt catgatccag gagcgcggca gcgagccgga
     1921 ggacgtggac ctgcgcgccc tcgagcgcgc gcagcccacg cccgaggtgc tggacgcgat
     1981 cgccggcagc gacgccgtcg tgatcggacc gtcgaacccg atcgtctcga tcggcccgat
     2041 cctcgaggtg cccggcatgc gcgacgcgat cgaggcctcg caagcgccgg tggtcgcggt
     2101 gagcccgttc gtcggaggac acgcggtgaa ggggccgacc gaggcgttct gccgcatggc
     2161 cggtctgccg gtcggcccgc tcgccgtggt ggaggcgtat gaggggctgg tcgacgggct
     2221 ggtgagcgac gagcccgtgc ccgacgcggg catgccggtg ctgcagatcc cgacgctgat
     2281 ggacacgccc gacgcccgcc gagagctggc ccagaggacc ctggactttg cgcgcgcttt
     2341 ggcctgacgc ggctccctca cgcgcgggcc aggcggtaga gtcgtgacgc ctgtcgctct
     2401 gcgccgatgc gtaccgccgc gatacttccc gtgaagagct tcgccgctgc gaagcagcga
     2461 ctgtcgccga ccctcggaag cggctcccgc caggccctgg cgctcgcgat ggtttccgac
     2521 gtgctcggcg ccctgcgacg cgtggacgga gtcgaggaag tcttcgtcgt cactgctgag
     2581 cgcaccgtcg aggccgccgc gcgaggcggc cgcgtccgtg tcctgcacga tcctgcggag
     2641 accgggcagt cgtcggccac tggcatcggc atcgaccgcg cagcggcaga gggcttcgaa
     2701 cgcgtggtcc tgatccccgg cgacacgccg ctcctcgacc cacgcgagct cgatgtgctc
     2761 ctcgcgcgcg cagaagagca gtctctcgac gttgcgatcg tgcccgaccg ccatggcgcc
     2821 ggtacgaacg cgctggtcct gcgtccgccg cacgcgatca ccccgagctt cgggccaggc
     2881 agcctgaagc ggcacgtgac cgcggcggag caggcctccc tccgattcga ggtcgtcgag
     2941 atcccttcgc tgatgcacga cgtggacgat cccgccgacc tgcacgacct ctccgcggcg
     3001 ctcgacgagc gccgcggcca tgccccgctc acccagggcg cgctccgcca gctcgggcgg
     3061 atgcgagcgg ctcctccagt cgaggtctag cactgccgct cacgatcctc gcgctcgcgc
     3121 ccctcccgga ggtgcgcccc ggcgacgatc tggcggcgct gctcgccgag gcctcgggcg
     3181 aggggctccg cgagggcgac gtgctggtgg tcgcgagcaa ggtggtgtcg aaggccgagg
     3241 gacgcatcgc gcggctgcgc gacgtggagc cgtcgccgcg ggcgcgcgag atcgccgccg
     3301 agaacgacaa ggacgcgcgg ctcgtccagc tcgtcctcga cgaatcgagc gagcttctac
     3361 gggccgagcg cggggtgctg atcaccgaga cgcgccaagg ctacgtgtgc gcgaacgccg
     3421 gcatcgacca gtcgaacgtg ccgggcgacg actcggtggt gcttctgccg ctcgaccccg
     3481 acaactccgc tcgcgcgctg cgagaggatc tcggtcgcac ccatggcgtg cgccccgcgg
     3541 tggtgatcac cgacagcttc ggccgcccct ggcggctcgg gcagatcgac gtggccgtgg
     3601 gcgccgccgg cctgcgcgtt ctcgacgact ggcgcgggcg caacgaccgc gagggccgcg
     3661 agctcgcggt caccgagatc gccgtggttg acgcggtggc cgccgccgcg gatctggcac
     3721 gcgcgaagga cgcgggtgag cccggcgtgc tggtgcgcgg cctcgagcgg ttcgtcaccg
     3781 aggagcacgg cccaggtgcc gccgcgctcc gccggccacg cgagcaggac ctgttccgct
     3841 agccgcagcg gcgccatcga gtcgggctcg ccgacgtcgg tgaacaccac ccccgcctcc
     3901 cgtcaccgcg tgtggatacc gccgcggatt cgcatgagcg ccgcgcgcag gtcctcgatt
     3961 ggaaggtccg ggttcagcag cgggctgtcc gcaccgtggg tgaggaggcg taggcaggct
     4021 gcggcgctga ccgggtccac gtccgtctcg ggttgctcga gctcgcgcgc gatctcgtcg
     4081 atgtcggcgg cgaacgcagc cacgcggtcg ggcgggacac acgacgttct ctgcgatgcg
     4141 tccgcaggca gggtccgcag cgactgtgac aaacgcttcc gtgctcgccg gctcgtcagc
     4201 cggcggccac ggctccgaac ggcctcgatc gcgtaggcgt cgcgatcgag cgtcagtcgc
     4261 tcgatcagga agtggcgctt caagccggcc acgagcgcca gtccgagtgc aacaaccgaa
     4321 ccgaaagcga gcgcgaagcc gagcgcagag gcgaatgtga aggccagacc cgcgagcgcg
     4381 cctatcagga gggcggcagc ggcatcgctg aactcttggc ggcttcgcac cgtcacctcg
     4441 cgatcgaacc tctcgaccga acgcgcgtcc acggcaactc cctgttggca tccaaaggtt
     4501 aactcgcggg cgttcatgag cggcgtctta tgatcgatgc catgaggctc ggcctgccga
     4561 tcgcggccat cgctgtcctg gtggctatcg ccatgctgtt gaccgagggg ctcacgtcga
     4621 gcgaccagga accgtcgcgc acggtcgttc agactccgcc cgcgacgaag gcgccgtccg
     4681 ggaagccgcc ggtgcaggcc gagggtgtga acacgacgca accggcgaaa ccggcatggc
     4741 ggggcccccg ccacctcact gcgctggtgc cccgccgcac ccagctgcgc gaccgtccgc
     4801 acgggcgccc catcgcggcg ctccccgcgc gcaccacctg gaaatcaccg cgcgtgctgt
     4861 tcgtcgccaa gcgccgcgac ggctggctcg gagtggtcgc acccgagctg cccaatgggc
     4921 acatcggctg gattcctcac agcatcgcgc gcatataccc cgtgtcgtgg gaactccgcg
     4981 ccgacctctc agcgcggcga gtgacgctaa tgcatgccgg ccgcgtggtg cggcgagtgc
     5041 ccgtcgcgat cggcgggccc gggacgccca cccccagggg gcgcttcgcc gtcacggaca
     5101 agctcgaggt gaaggcggcc tcgccctatg gctgctgcat cctggcgctc tccggccacc
     5161 agccgaacat cgcgcagggc tggggtggcg gcgaccggat cgcgctgcac gcgaccacga
     5221 acgaggcgac ggtcggcagc gcggtgagcc acggctgcct tcgcgcgcgt gcgcgcgaca
     5281 tgaaggtgct gattcgcgaa gtgccgttgg gcacccccat ttacatcaag gcgtagctcc
     5341 ggtccagcta cggcatcgtg ggtcaccgcg tagcgcttgt cagcgctgga tcctcactac
     5401 gcagggcgtg gttccggtcc agctacggct tcaggcggcc gcgggcacct cgaaccgccg
     5461 gcggatctcg tagagcgtcg tgcgctccgc cgccgggcgg cccgccgcgt gcgccgcgcc
     5521 gatcaggtcc gcgggctcga gccggacgcc gtggtacgag ccggccatgc ggctgatcga
     5581 ctcctccatg agcgtgccac caaggtcgtt cactccccag cgaagcgcct cggtcgccac
     5641 gtcgaggccc atcttcaccc acgacgcctg caggctgggg atcgtctttc cgagcgcgag
     5701 ccggaacgcg gccgtgtgct tcaggttgtc gtcgcgggag atctcctcga tcccgtgcgt
     5761 acgaccgagc agtgtgtgga atgggatgaa cgagagcggc acgaactcgg tgaagccgcc
     5821 ggtgcgctcc tgcagctcgc gcaccacgcg catgtgctcg gcgagctccc acggctcctc
     5881 gacgtggccg aacatgacgg tgaccgtcga gcggaggccg gccgcgtggg aggcctcgat
     5941 gatctcgacc cagcggcgca cgggcagctt gttcggagag atgcgctcgc gcacaccgtc
     6001 gtggagcacc tcagcggcgg tgccaggggt cgagtcgagc ccggcgtcgc gcaggcgcgc
     6061 gaacacctcg agcggcggca gtcccgagac gtcgcagatg tgagccacct ccatcggcga
     6121 gtaggcgtgg aggtggatgt cgggcgcgac ctccttcgcg gtgcgcagcc acttctcgta
     6181 gtcctcgatc gaccagtccg ggtgaatgcc cgactgcatg cagatctcgg tggcgccgaa
     6241 ctcgactgcc tcctcgaccc ggcggacgaa ctccgcgcgc gagtgctcgt aggcgtccgg
     6301 cgagcgcctg ccctgcccaa agccgcagaa cgcgcacccg acgatgcaga cgttggacac
     6361 gttgatgttc cggttcacca cgaacgtgac cgtgtcgccg gcgagctccg cgcgcagctc
     6421 gtcggccgcc tgccgcatgt cctcgagcgc ctctgggcgg ttctcggcga acagcgcggt
     6481 gagctcctcc tcgttcagcc actcaccgtc gcgggcgcgc tcgatcgcgc ccgggacggt
     6541 gtcctggcgg atcgcccgct cctcgcggcg gcccgaccca cggcgaggga tgaagctcca
     6601 gtacttcagc ttgatcacgt cgagcacgcc ctgctcgagc cactcgggcg tcatgtactg
     6661 cgggtacacg cagagccgct ccgtgagcgc gtacccgcgc ggagcgagct gcttgcgcat
     6721 cctgtgcacc gacgggaaag ggtgctcggg ggagatgtgg tcgccgttcg ccgacaggcc
     6781 tccgaggtcg gtggcgccct cctccacgag cggcagccac cagtcggaca ggttcggcgg
     6841 gatctggacg cccacgtcgg gcatcagccg cttcgactcg cggacgagca ggcgcatgtc
     6901 gtcgagcgag atcggtttcg cccagtcggg tagcgggagc gcctcgccgt gcgtctggct
     6961 gcgctgcgcc tcgtcggcga tctcggcagg ctcctgtccg tagtagcgcg gatgcggcac
     7021 gaagttctgg aggatgacct cctggatgtg gccgaactcg gcctgcaccg cggcgatcgc
     7081 ctcgagcgcc gcgatgcgct cgtccggctc ctcgccgatc cccacgagga tgcccgtggt
     7141 gaacgggatg cgcagctcgc cggcggcgcg gatcatgtcg aggcgccgcg ccgggtgctt
     7201 ggtgggcgag ccctggtgca cgaccaggtc cgggttcacc gactcgagca tcagcccctg
     7261 cgaggccgtg acctcgcgca gccgggcgag ctcgtcgcgc gacgcgacgc cgatgttcgt
     7321 gtgcggcagc atgccgcgct cgagcgcgcg ctcgcacgcc caggcgacgt agctcgtgaa
     7381 gtccgcgtgg ccgagctcgc cgagcctcgc cgccacctcc gggttcacct ccggcttctc
     7441 gccggtgagc acgagcaact ccttcacgcc acggcgagcg gcgtcgtcga gcatcttctc
     7501 gacctcatcc gccgtgtaga gatgaggccg gtgcgtcgcg aacgcgcagt acttgcaata
     7561 gcactggcac gttcgcgaca gcgacagcgt gaagttgcgg gagaaggtga cgcgccgcac
     7621 tcgggcgcag tctaaggaac cgggcgcgcg gtctccgagc tgccggtctc cgagctgcag
     7681 cacttacggg aggaggatgg atgactttcc accgctatgc ggggcaaagg tcatccgcgt
     7741 cgacaatccg ggggtggtgc cactgaggtc ggatagcggc gtcacagtca gcaagacgtc
     7801 gctgggcggg ctcgatgcgc ggtagctgct gccgactggc agttcgaggc ggcggccagc
     7861 tcctcggagg ttgcctgctc gaccgtgcgt cgcgcctggg caacccacgt cggcggcggc
     7921 gccgacgtgg ggggagcgcg tcgaggcgct cgagcatccg gtcacgcgcc tgctcggtgc
     7981 cgcgcgggcg aggcggaggc gtagtgctcc tcccgcacgg gcagctggcg agcagctcaa
     8041 cgtgtagcgc cgctcgccgc ggcgcgcgcg gcgctacggc cgcagtgagg gcggggtccg
     8101 gcccgctccg cccagcaggc aaagccttaa cagatacgac gatgaaggac cgagtttgcc
     8161 cgtcagtgcc aataccgtga cgcgacgagt cacctacttt tcgtcccggc cgcttggcac
     8221 ccggacgctt ccggaagtag cgctcgtaca tccgtccaac tcatttccga tttcgcgaag
     8281 cgcgccccta aacgtccgca ctggcgaaat ccctgtaatt gcatgacatt cagatgatcc
     8341 gtgtcgacgc agaccattca cctgtcaccg gtttgtggga ttgacgcgcg aagttcgtct
     8401 gatcaacatc gacggccgtt ttggtcttcg acgaggagta cttcgtttgc gtccgttact
     8461 ggttctccaa cctgcccgta caggcctatt cgcgatcgcg ctttgctgtt gttcgttcgt
     8521 actcttcgtg tcaacgcaaa ccgccaacgc agcacttcca ccaagcgtcg agtcggcact
     8581 ccaagatggc aagattcagc agtgggcaga acgaggcggt gcggcacagc tgcagggcgg
     8641 gggtgcgtgg ccctgcgccg aatactgcga agcaacatat tcggcggagc agtccgcgcg
     8701 caacggggga aatgcggcac ttgcctcatt gctagcgaag caggtctaca agctccggac
     8761 catggcgaag ctcgataccc cgccgggtgc tatcggcgca ctcgtgctcg gaccgcaggc
     8821 gttcatcaag ggcttcgaca ttgggagcgg cttaaaccgt aaatacttca ggctcggact
     8881 gcccgcgaaa ggcgttggtt cccccgcgag ccccgccctc aggtttctga cgcaggggac
     8941 ccgagttggt acgggggcga acatggttat gccgaaggac ggctgggtgc tgtcgtggtg
     9001 cctcggctgc ggctcaacgg agttcttggt gttccccgat ggcagcgcct ctaacggcga
     9061 ctgcatatcc gcgtaccagc agccgccaca cggtcttagc gcgctcatgg gcgactattc
     9121 cgcatgcaat acacacaccg ggccctatcc cggcttggtc gttgacatgc aatacgccgc
     9181 gttctggact gacgagtatg gctcagaggc cttcgactgg tatcccatgg aggactacac
     9241 aggccaacca ggcacaacgc acatcccaga tccatcttcc ttatctcagt cgacggcgga
     9301 cgccaacgta gctggggagc taaggaacaa tccggacgag tactcgatcc tcatcccttg
     9361 gcttcggtgg acaacgggcg aaccaaacgc ctgtgatccg acgaatccgt tggtttgtaa
     9421 tccggatgac gagcagaatc gtcgtgaatg tgagctatcc gaccacctga ccgagctgga
     9481 caatccgacg gcagatccgt acgacgtgca cgacgacttc actcggctcg actgggacgg
     9541 cgacacgatc cccacggcgc tgaaatatgg aaccgacagc tggggctacc gaaagatcgc
     9601 tccaacacat ggatggggtc cgcccgatgt cctccagaca caagccgccc tgaacggacc
     9661 acctgacgac cccacgttca aggggagcgg aaccgaggtc tacgacggtg cgacgtacac
     9721 cggcaccgat ggttcaatct gcttccgccg ggtgatagtg cgacacacgg cgaacctcgg
     9781 cgaggtcgcg ccaaaggaga tcatcagctc ctatggccga aaggttccat aaatggcgga
     9841 cgaagatcga aagcatgaac tagcttgcct cgccgcaacg acggcggtgc tcgacggcgc
     9901 caagcgattg ctcggacgga aagttgtctc gtctgtccgc ctcgaaggcg ccaagcccga
     9961 gacagaaatc gttgtccagt ggcgatggga ctacgaacaa gagccacacg agcaacgctt
    10021 caagttttgg tctgccgact ttgccgagtc ggacggaacc ccaggtcgcc cggacgaagt
    10081 tgctgccgtc atcctcgcaa acgtcgaagc agagcagtag aacgagcgga actcagcccg
    10141 agtcgcggcg acctcaggcg cgcatagcag ccagcgccaa atacagctcc cgcgccgagc
    10201 gggctcccat ctcgaggcgg tccaagccga agctctcgtt cggggcgtgg agggcgtcct
    10261 cgggcagcat gaagccgctc aggatcgtcg ggatccggcg ctccgcgaac gccgccagga
    10321 tcgggatcga gccgccgctc cgcacgagcg ccggcttcac gcccgtcgcg cgctcgatcg
    10381 cgtcggagcc gagctggatg gcgggcaacg taggatcgaa gagagcgggg tcggatccgt
    10441 gcatgcggat gtccacgtcg gcgccttcgg gcaccgcctc gcggagcagg cgctcgagca
    10501 ccggcgccat ctcggaatac tcctgcccgg gcgccagccg catggacagc ttcgcgcgag
    10561 cggtcgccgg cacgatcgtg cgttcctgct ccgcctcgcc gccctcgatg ccgttcacgt
    10621 cgatcgacgg gcccgcccac gtgcgcgcgt agaagtcggc gacggcgtga ggaccgagcg
    10681 gccgtccacc ggactcggcg atcgcggcgg ccgcatcggg cagcgaacgc catccagcga
    10741 gctcttcggg actcggcgaa gccactccct cctgcagctc ttcgcgcagc accccgtccg
    10801 gccccggcag cacgaccgag agcacctcgt gcagcacatg gaccgcgttc agaaccgccc
    10861 cgccgtagag ccccgaatgg aggtcggcgc tggccacccg cacgtcgatg gccacggcca
    10921 ccatcccgcg cactgcaacc gttatggccg gcgtgcgcgc atcgagcatg ccggagtcga
    10981 acacaatggc ggcgtccgcg ccgcgctcgt cgcgcgcgat ccagcgcatc acctcgcggc
    11041 tgccgacctc ctcctcgccc tcggccagca cgcgcacgtt caccggcagc tcgcccgcgc
    11101 gcgccatgtc agcggccacg tgcagcagcg gcaggaagtt gcccttgtcg tccacggcgc
    11161 cgcgcgcgta gaccttgcca tcgcgcatat cggcatcgaa cggaccggac tcccacagct
    11221 cgagcggtcc gactccctgc acgtcgtagt gcccgtagat caggatggtc ggcgccgact
    11281 cagggtcagt cgacgcgcgc agctcgccca cggcgagcgg attgccgtcg gtctccacga
    11341 gctcggccga cccaccggcc gccgcgatgc ggtcgaccac ccactgtccg gccgcgcgaa
    11401 ttgcgtccgc gtcgccgccg ccggaggaca ccgacgggat ccggagccag tcgatcagct
    11461 cgtctacgag ggcgggcacg gccccgaaaa ctaaccgccc ttgacctcct ggaccgtgtt
    11521 gcccccgtcg atcacgatcg actggccggt cacgtagctg gccgcgtcgg acgcgagcca
    11581 ggcgatcagc tcgccgacct cgtcgggcgt gccggcccgg ccgagcggcg tgtgctggcc
    11641 tgcacgccgc tccatcggcg aaagcgacgc tgttgcgatc cagcctgggg cgacggagtt
    11701 cacggtgatg cccgacggcc cgaggtcgac cgcgagcgcg cgcgtcagcc catccacccc
    11761 agctttggca gcgctgtacg gcgcctgcgc cggatagctc acgtgcgacc cggtcaccga
    11821 gctcacgttc acgatccggc cgtagcggcg ctgcaccatc ccaggcgtca cgccgcgcgt
    11881 cacgtaatag gtggtgagaa gcgtgatcgc gagctcgcgc tcgaacgcgc cctcgccaag
    11941 ctcggtgaac ggcttggact tcatctcctc gttcaccgtg gtcatgcccg cgttgttcac
    12001 cagcacgtcg atctcgccga ggcggcgctc cacgtcgttc agcatctgat cgaccgcgtc
    12061 ggggtaccgc aggtcgcaca cgaagccggc cgccgcatcg ccgacctcca gcgcgcgctc
    12121 gtggatgcgc tccgtcgtcg agacgatcgc gacgcgcgcg ccccgctccg cgaggatgcg
    12181 ggcagtggcg aagccgattc cctcaggact gcccgcgccg gtgacgagcg ccacccgctg
    12241 gtccagccgt atccggtcgc tcacgccgcc agtgtctcag ccgcggcggc gaatgcttca
    12301 gtatgggcgt ccacgtcggc ttccgtcgtc gccggacaca tcagcgccat gttgtggaag
    12361 ggcgtgatca gcacgccgcg attcatgagg aagacgtgca tgaacgcgtc gagctccggg
    12421 tccgacgcgg ccgccgcctc gccgccgttg cgcggcggct cggggcggta gcggtattcg
    12481 gcgcgcgctc ccagtcgcac cacgtgccag ggcagcgagt acgttgcgat cacgtcgttg
    12541 acgcccgccg cgaagcgctc cgcaagcgcg gtcatgcgct cgaacgcctc ctcggtgagc
    12601 acgtgctcga gcgtcgcgcg cgccgccgcg agcgacagcg cgttgccggc gagcgtgccg
    12661 ccgacaccgc cgacatcctc gaggtcgacg cccggcagcg tgagcagccg ctgctcgagc
    12721 tcctcgctca tcccgtaggc ggccacgggc acgccgccgc cgattgcctt gccgaccgtg
    12781 agcaggtcgg gctcgagccc atgcgccgcg gtgtagccgc caggccccgc gctcatcgtg
    12841 tgcgtctcgt cgatcaagag cagcgtgccg gcgtcgcgcg tgagctgccg caacgcgtcg
    12901 tgaaagcccg gctcaggaag gacgatgccg atgttcgtga gtgcgggctc ggccagaacg
    12961 cacgcgatct cgccgccgtc cagcgcgcgc tcgagcgcct cggggtcgtt ccactcgacc
    13021 acctcgctgg tcagcactgg gtcgacagcc ggacccacgt tcccgtgccg cgagcgcggc
    13081 cgtccacgct cgtccagcga gatgaacgtc tcgtcgacgg tgccgtggta gcagtagttg
    13141 aacacgagca ccttgggccg tgccgtgatc gcgcgcgcca tgcggagcac ccagcggttc
    13201 gcgtcggtgg cggtgagcgc gaactgccag cgcggcaggc cgaagcgccg tcgcagctcc
    13261 tcgcccaccc aggcggcgtc ctcggtcggc agcatcgcgg tcacgccgcg gcgcgcgcgg
    13321 gccgagacca cctcggccac cgcctctggg gcatggccgg ccatcgcgcc cgtgtcgccg
    13381 aggcacaggt ccacgtactc gcgcccgtcg gcatcggtga tgcgtgcgcc gcgggcttcc
    13441 tcgaagaaca gcgggaagcc gcccgcccac atcgtcatcc agctcatcgg cacgccggca
    13501 acgagcgacc gcttcgagcg ctcgaagagc tcgcgcgagc gcgggtgatc cgccgcgaag
    13561 cgctcacgct cggcggcgag cagctgcgcg agcctcccgc ggtccattcc gggaagctaa
    13621 ccgagaggtg cagcgcccgc cgtacaagac ggcgggcgcc agttgtccta gccgaccggt
    13681 tccatcgcgc cgacctcgcg tcttcccggc gtgacgaaca ggtagacgta cgtgagcgcc
    13741 gcgagcgtgg cgccgacgag cggaccggcc acgtagacga gcaggaactt gccggcgccg
    13801 ccgaagtcgc tcgccacgag agccgggccg aatgcacgcg ccgggttgaa gcccgcgccg
    13861 gtgagcgagc cgaagatcat cacgaggaag ccaagcgttc cgccgatcac gaagcccgac
    13921 cagtccttga gccccgccgg gttcacggcg acgccgacga tcgcccacat caggaagaac
    13981 gtgccgatca gctcgcagac gaacccgagc cccgccttgc cgtccagcag gttggagacc
    14041 ccgaccgcgc cccagttcac gtcgcgcccc tcattcagca gaagcgcctt cgtgagaagc
    14101 gcgccgagcg tgccgcccgc cagctgcatc gcccagtaca cgaccgcgtc ggccggcttg
    14161 atctgccgga tggccgccat ggcgagtgtg accgccgggt tgaagtgcgc gcccgagatc
    14221 accgcgagcg tctggatcag catgaagagg acgaagatgt ggaccagtcc gaccaccgac
    14281 cagtcgatga acggttgtgt cgccgcgccg ggcgtgggcg gggtcacgaa cagcgacacg
    14341 accgcggtga tgaagaacac gagcgcgaag gtgccgatga actcgcctat gtatgcggct
    14401 acgccgcgtt cctccatggg gcttcctcct ggtccaggtc gacgatgcgc ggcagattat
    14461 ttgcgccgcc ggtcggcaag cgcgcgccgg cgctagcgcg tatcgatcat gagcggccgt
    14521 ccggcgttgc cctgctcgtc caccgcgcgc accgctatgt agcgcgcgtg cgcggccggc
    14581 agcgtgagcg tctgcagcgt tccggcctcc gccggctccg gcgccccgtc caccttctgc
    14641 gccgagccga agctcgcctc gtccaccggc ttcgacgact gcacgacctc gtagcgcgcc
    14701 gccttgccac agagcaggtc gtcaccgggc gcgcggaagc gcaccgcgct cgagccggac
    14761 ttcgacccgt cgtagggacg gcccggcacg accgcgtcgc ggttgaagtc acccgagctc
    14821 gcgttgtcgt ggtggaagcg cggccacgag ccgggcgagc acgacggcgc gtccacgccc
    14881 cacgcgaaca cgccgcccgc gcgcgtctcg ttgacgacga ccttgtgcgc tccgtcgctc
    14941 gtgtcgagcg tgccgaacga gccgatcaac gggttcgcca cggtccagtc cgagttcagc
    15001 ttcggccagc ggctgttcac cggctgcccg gcgaagtcgt acgcctgcac gtcctcgtac
    15061 gcgctcgccg acaccgactc ctcaccgggc atgccgtcga tgtccgcgat cgacggccct
    15121 gtgaggaact gcaggtcgtt cgtgtgaccc ggccagcccg gctggaactg cccggtggcg
    15181 gggttccaag cgcccacgta gtcctgcccg ccctggtact ccggcgcagc gatgtcgagc
    15241 gtccgcatca ggccgatggc cggcgcgagg aacgacgcgc tcgagccgtc gagcttgccg
    15301 aacgccgaca tgccgagccc gggcacgatc ggccgatccg tctcgtcgac gccctggccg
    15361 gtgtcgctca gcgcgatcgg cttgccgtcg ggaccgttcc cgtagcacga ggtgccgtcc
    15421 ccgttgagga tgtaggcagg cccgccgtca gggatcacgc cgaccttcgt gcctgagccg
    15481 ccgctcgggc agttcaccgg ccccgtcgac ggcggcccgg tgatgccctc gccgacgacg
    15541 ggcaggatct ccttctggag cagcgcgacc ttgaacggcc agccgtcgac gaaggcgtcg
    15601 cccacggtct gcgcgttgcc gtcggggtcg ccggtgggct tgattgcgta gatgcggctg
    15661 ttcgccggcc cgaggacggt cgcgagcgcg ccgtaggagg cggcgttgaa ctcggacacg
    15721 ttcggcccgc catcgccggc gccgtcgtac tcctcgttgg tgccgaccac gatttcgggc
    15781 cggtggtccg cgttcagatc cgccagggcc ggcgtgtcga cgatcgcgcc ctggttgagc
    15841 gccggccctg cgctcgactt gaagtgcacg acgtgcgaac gcgggtcgac cgagtcgacc
    15901 ttcgcggggt ccaccacgag caccgggaag cccttcactt gcgagccgtc ggcgtgccac
    15961 gcgtacatgt gacggtccat gccggcctgc acgacctcga gcttgccgtc gccgtcgagg
    16021 tccgcgagca ccggcgacgc gatgaagccg tgctgcgtgc ggtggcgctg cgactcgtcg
    16081 gggttttgcg cgtcgtagcg ctcgggcgca aacggcgaga gcggcttgcc cgaccagtcc
    16141 gggttcgcct cgcgcttcca gacgagtccc ttcgcgctcc agacgtacac cttcccgccc
    16201 atgtccgagc cgacgatctc gggtgagccg tcgccgttca ggtcggagac cgcgaccgaa
    16261 gagagcatcg ccgtgcgcga cgccgacgtg gacacgtcgc cggatgtgaa cgcccgctcg
    16321 ccggtgtgta gcggcagcgc gtcggtgagc accgggaagc cgggcagctc gctgccgtcg
    16381 cgccgcatcg catgtacgcg tccgtccgag cttccgaaca cgagctcgtt gcggttgtcg
    16441 ccgtcgaggt cggcgaacag cggcgacgac gcaccgtcgc ccgtgagctg gcgcgggaag
    16501 ccgggcagca ggtcggagtc gcggtgtagg tacatgttgc ggcggtcgtc gccggtgagc
    16561 ttcgtgccgc cctgctcgct cgtgactacg accttcaccg tgaagccgta cgcgtcgttg
    16621 gacggacgcc cgttcgaggt ctgcggcgtg gtgccgggct cgcggccctg gaagtcgccg
    16681 gcattcgcgg ggaagcggcc cttcaagtcc gccacgtcga tcttggcgag cacgcccttg
    16741 aagctcgcgg tgtgcgtaga gtcgccgcag tagccgccgc cgaccggctt gaagtcgccg
    16801 tcgggggcgg tgccgtcgtt cgggtacgag ccgggcgcga cgtacacctg gcagcggtac
    16861 gagttcccgc gcgcgcccac gaagccggtc acgggcgtgc tcgccgtgcg cggatcgatc
    16921 tgcgcgtacc actcgggcga gtacagctcc gcctcaggcg ggagcttcac cgtggcgtcg
    16981 aacacaccga cgctcttcgc ggcgttgatg cgcccgtagc cgtagtacgg gtcgggtccc
    17041 ttgcgggcgg tgtagcgccg cgatccgggc aatggcagca ccgcctggtg ctgcggcagg
    17101 cgcggcgcga agttgggatc cgtgcagaac ggcgcgccgc cgcccgccgg cgacgagcac
    17161 gacggctccg gttgcgcggt gaacgccacg tcgtcagaac gcatggccat cagctgacgc
    17221 acctcgttcg ccgagatcac gcacgagtca ccgttcacgc gcttgcagtc cggattcgtg
    17281 ccgagccgcc ccttgtcgtg ggcgtcgagc gccgcgctgt agatgagccc ggcgatgccg
    17341 gcgccgcggc cggtcgcctc cgacgagcag ctcgtgctcg ggatcgacac cgtgatcttc
    17401 gacgaaaagt tggtgcagcc gttgaactgg agatatgagc gcggttgcgc agtgaaggtg
    17461 tcgtcgtact tccgcacgga gttgacgacg atcacgtgcg gataggtcga cggccagttg
    17521 tggtgctggg cggactcgtc cgcggcggag gcgatcaccg ccacgccgtg gtcgtacgcg
    17581 tattccaccg cgtcgcgcgc gagcttgctg ttgttgagcg tgccgagcgc ctcctgcacg
    17641 accagcatgt tgttgtcgac cgcgtagagc accgcctgcg cgaagtcgtt cgagtcggcg
    17701 atgaaggagt cgcctacgcg cagcggcacc gacatgcagt tcgggcaggt gccgaggtcg
    17761 tcgccgttgt cggcctccgc ggtcgagtcg cggagctcac ccgtgccgtg gccgtactgc
    17821 acgtcgtcga acgggtcgtt gtcgttgtcg aggaagtccc agcccgcgat gtcgtcggtg
    17881 aagccgttgc ggtcctggtc cctgccgtcg ctaaaggcga tcagtacgtc ctgcgggtcg
    17941 agcagatcgc ccgggccctg cccacgcggc tggcccttcg ccgcccgcgc ggcgggatcg
    18001 cgttccacgc gcgagtcgca ggcgtagtcc gtcacgttga acacgccgtc gccgctcgcg
    18061 tcgtaggccg acttgtagga ggagcagtca gcgccctcct cgagcggcgt ggtgcggtcg
    18121 tggttcggca ccggcagctc gcccttgttg agacggatct tgtggcggag gtcgagcatg
    18181 gccccgcggt cgttccactt cacaccggag tcgagctcgc cgatcacgac gtccggccgg
    18241 ccggttgtgt gctgccaagc cgtgtccacg gacgggtcct tgtccacgat gtgcgcgccg
    18301 cgcacgccgt tcagctcggt cggatccgag ttcaccgcga ggttgcccgg ctcggacgtc
    18361 gcggcgtact tccactccgt gccgctgtcg aggtcgttcg gcacctgtcc cggtgccgtg
    18421 tgcgacggat atggaaaggc cgccagggcg gacggcgtgt acgccgccag cgccagcaga
    18481 ccgcacgcca ggactcccgc aaccctgcgc aacagctacc ccctgctcag ttccccctgc
    18541 cccttgcgcg ggacaaggta gagcgtggcg cccgcgcgtg cgccggttta cacgccgctc
    18601 gcgccgcggc gcacgaggcc cggcgcgaag gtcttgagcg cggggatcag cgcgtagccg
    18661 cgcggcacga tcacctcggg tttcccgccc ggccccgcct tgaagatcgc ctccgcggcc
    18721 ttctcgggct tcgaaacgat ccagcgcggt ttgatgtcct tctgcgggaa gccctcagtg
    18781 acgatgaagc cgggcagcac catccccacg tgaacattgt tcggcttctc ctcgaggtgg
    18841 agcgcgtcgg tccaggacgc cagcgctgcc ttcgccgccg agtacgcgcc gctcttcggc
    18901 cgcgaagccc gggcggcgac cgacgacacg ttcacgatcg cactcggcgc cgacgcacgc
    18961 agcacgggaa gcagcgcctc cgtgagccgg acgacggcgt cgaagttcaa ctccatcgtg
    19021 cggcgcacgt tctcccagcc gcccttctcg tcgtcgccga acgcggcgcg ccaggcgccg
    19081 ccggcgttgt ttacgagcag atccagctgc ccgccgaact cgcggtccac cgcctcgcgc
    19141 acgtgtgcgg gcgcgtcgcc atcggtgagg tcgagcgaga gcgggatcac ggcgtcgcct
    19201 agctccgctt tcagctggtc gaggcgttcg gcgcgccgcg cgaccgcgat caggcggtag
    19261 ccgcgctgcg cgagcaaccg cgcggtcgcc tcgccgatcc cgctcgacgc gccggtgacg
    19321 atcgcgttct tcacgccgcc agtctgtcaa ccagcgcggt catctcgcgt gccatccgcg
    19381 agcgcggcac gacgagttcg aagcccgcct cttcggcgcg ccgccgcgtg tcctggtcca
    19441 cgtgcgagta gaagccgagt cgcggcgcgc cgtccggcgc gcgggcggcc gagccgtcga
    19501 actccggcgc gttcaagtcg accacgagca catccgcctc ggcggcgctt gcgtcctccg
    19561 gccggtcgaa ccgcgcgacc tcgtgccccg ccgcacgcag cccgccttcg agcttcgatc
    19621 cgaagagcag gtccggacaa agcagcgcga cgcgcgccat cgtcactgcg agcgccggcg
    19681 cgcgaactct cggcggaccg actcgggccg gccccacccc tgcgtgatct cgacgcggcc
    19741 ggagcgtgcg tcgcgcgcgg ccagctcgcc ctcgatgccc tgctcgtcga gcagcgcgag
    19801 cgtgcccctc accgtctcgg ccggcgcggc gtggccgaac tcgtgcgcca ccgggagccg
    19861 ccagtgccag tcgtgcttcg agtacggctg ggcgttgatc tgaacgagga tggtggccgc
    19921 ctcgatgtag cggtcctcgt cgaagatccg caggtcgcag acgaggttcg tccagtcagg
    19981 cggcagcgag tcgacaatct gctggaagtc gtcggcaagc gccacgcggg gcagactagc
    20041 ggccctccct agaatcggct ccgtgaccgt cgcaaccgaa tatgagctgc tccgcggcag
    20101 cgcgggcctc gtcgaccgcc cggagcgcgg caagctgctc ctgaccggcg cggaggcagc
    20161 cgagttcctc cagggccagc tgacgaacga catcgaatcg ctcgcgcccg gcaccggctg
    20221 ctacgccgcc cttctcaccc acaagggcaa gctgctcgct gacatgcgcg tgctgcgcgg
    20281 cgaggactgg atcctgatcg actccgaggc ggcggcgatg ccggcggtcg cgaagaacgt
    20341 gcgcatgtac tcgatcgggc gcgacgtcca ggcccgcgac gtaagcaccg agcgcgcgat
    20401 cctctcgctc atcggaccca cctcgcgcga cgcgctcgac gtcgcgccgc cggacgcgga
    20461 gcactcgttc gtggagggtg agcacgggct ttacgtgacc accgatctcg gcgtggacgt
    20521 gatctgcgac gcggccgacg ccgacggagt gcgcgcggcg ctcgggatcg acgaggtgtc
    20581 acccgacgcg gccgagctgc ttcgcgtgga gtccgggcgg ccccggctgg gggtcgacat
    20641 ggacggcgac accatcccgc aggaagcggg gctgaacgag cgcgcggtga gcttcacgaa
    20701 gggctgctac gtgggccagg agaccgttgc tcggctgcac tacaagggca agccgaaccg
    20761 tcacctgcgg ggccttcggt tgtcggcacc ggcgtcccat ggcgatccga tcgttctcgg
    20821 cgaccgcgag gtcgggcgta tcggctcggt agccgactcg ccgaggctcg gccccatcgc
    20881 gctcgcgatc gtgcgccggg aggcgtcgcc gggcgacacg gtgctggtcg gcgagggccg
    20941 tgcggcggcc gagctggccg agcttccctt caccgcgggc tgacgttaac cccgcaagct
    21001 cggtgatccg tccgcggcaa ccgggcgcac aagctggtat cgcacgcctc cagcgtgggt
    21061 agtatgggcc gcaagtcgtc ggaagatccc gggccaggcg gaggagccat gacgacgctc
    21121 acgcagtctc aggtgaatag gaagcttgca gagctcgatt tgctcgcgca ggaggcttgg
    21181 ctcgactacc gcgacggtct gcgtgagctg gatgggcgcg aatacgaaga cgccgaggac
    21241 cagtcgtggc tacgactcca agagaggctc caggagctcg acagggagcg ccagctgctc
    21301 gtggcaagct gagcgcggac agccctctgc tgggcggtcc gctcgcccac gacccgcgcg
    21361 aagacgtata cgagttaccg cagccgccgg cacccgagcg cgagccgggc accgacctgc
    21421 gacagctccg ccggctgctg cccgcgacgg agccgagccg cgccgtcgac gactggggac
    21481 gcagcgagcg catcgtctcg ctcatggagc cggtgctcga cttctattac gactactggt
    21541 tccgggttga ggtcgaggga atcgagaacg tgccggccga gggcggcgcg ctgctcgtgt
    21601 cgaaccactc tggcgccctg cctcccgacg cgccgatgat catatgggcg atccggcgcg
    21661 agcatccggc gccgcggccc gtctacatgc tcggcgagca ctggttcaag ggctatccgg
    21721 gcgtgggcat gctcgcgaac aagatcggcc tggtcgcggc gcaccccgcg aacgcgcagc
    21781 ggctcctgcg cgacgagggc cggctcgtcc tcgtgttccc ggagggccag aaggggtcgc
    21841 gaaagctgta ctggcagcgg taccggctgc gccgcttcgg ccgcggtggc tacgtgaaga
    21901 ccgcgctccg gtcgggcgtg ccgatcgtgc cgatcgccgt gatcggagcc gaggaggcga
    21961 tgccgatctt cgcccacgtg ccgctgttgc agcggctcac cgggctcatc tacttcccgg
    22021 tcaaccacgc gttcccgcac ttcggcctgg ccgcggctgg gatgtacctg ccggccaagt
    22081 tcaagatccg gttcctctcg cccatctcgc tcgacgacta cggccccgag gacgccgacg
    22141 acttgcagct cgtgcagtcg ctctccgagc acgtccgcgc gtgcatccag cgcgaggtcg
    22201 atgcgctggt agctgcgcga aagtccgtgt ggttcggtta gattcctcgc cgaggggatg
    22261 aggatgcgag ccacggagcg gttcagcagg ggcacacgcg tcgcggtcgc ggcgtccgcc
    22321 gttgcggtgt ttgcggcggg ctgcggcggc ggcagcgact tccagaacaa gccgcgcccg
    22381 gcggttcccg ttcagctgac cggcgtgatc acgcgggaca aggtgaccgt ctcgcccgac
    22441 agcgtcggtg ccggccccgt gatcatcgtc gtgtcgaacc agacgcctga gaagcacacg
    22501 ctcacgctcg acggcggcgg cggaactcag caggtccggg tcggtcccat tgcgccgacg
    22561 gaaaccggcc gcatcactgg cgacctcgcc cccggcagct accaggtgaa agccggcacc
    22621 gagcaggccc aggtgcgcga gatcgccccg gcgacgctgc acatcggcaa ggcgcgcaag
    22681 agctcgagcg acacgctgct gctgccttag gcgcttgcgc cctcggcggt ccatttcttc
    22741 catcgaaaga gcagtcagga gatcgcgatg gagcatgtgg tcctatgcgg accatttctt
    22801 ccatcgcgag tgccgaggag cggacgcgat gga
//
LOCUS       DASVXS010000136         4912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_462277, whole genome shotgun sequence.
ACCESSION   DASVXS010000136 DASVXS010000000
VERSION     DASVXS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462277"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VF752_07450"
     CDS             <1..435
                     /locus_tag="VF752_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8855422.1"
                     /translation="LGALVHHIMRTCSGGVLEAVDKLGLSLSQLKALTTLTGSESLEM
                     SLKEIGDALGLSLPAVSRAVDGLVQRDLVTRTEDPLDRRSKRVAATATGRALLDELTT
                     LRLVDLEQFVETLTPEQRELLANAVTAITARDDIKTLPPRKD"
     gene            440..2029
                     /locus_tag="VF752_07455"
     CDS             440..2029
                     /locus_tag="VF752_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855423.1"
                     /translation="MPERIRLTEDNRKWWTLAAMCFALFMVMLDNTVVNVALPSIQKD
                     LGASITSLEWTVNAYTLTFAVLLVTGGRLGDIFGRRRMFLFGVVLFALSSAAIGFAPT
                     DTLLVAGRAIQGIGAAFMMPGTLSIISNTFPPEERGRAIGTWAGVSALALAVGPVVGG
                     ALTEYVSWRAIFFLNLPVAVGAVAVTLFAARESRDNTIERKVDIPGIAALSVSLTALV
                     LALVEGNGWGWTSGRIIALFTVAAVGFIAFALTEMRVRVPMVQFAFFKSRSFVGANVV
                     AFIVSFAMLAMFFFIALYMQNILGYSPLEAGVRFLPSTAMIIIAAPIAGRLSDRIGPK
                     PLIVTGLGLASFALYLQSTIDVNTGYGHLLPAFIIMGIGMGLTMSPMSTAAMNAVSVD
                     KAGAASGILSMSRMVGGTFGVALIGALFESTSRNRLADSLGKLSLTPGQRSSITDQIA
                     TGTGGQPKGVPGAVATQVHAASNDAFIHALSSSLTVATFVAAFGAIAAAALIERKRPA
                     RSREQELRQSPEAVAAEELAA"
     gene            complement(2133..2396)
                     /locus_tag="VF752_07460"
     CDS             complement(2133..2396)
                     /locus_tag="VF752_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855424.1"
                     /translation="MLEQVSAAVARAGVDLDSAADVTILVPPFGADDDSTMHDGMDAY
                     VSLHGGADGHVRRALSAGNAVIEPSAQSIADWLRGVSNTRRAA"
     gene            2645..3898
                     /gene="ccrA"
                     /locus_tag="VF752_07465"
     CDS             2645..3898
                     /gene="ccrA"
                     /locus_tag="VF752_07465"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX8855425.1"
                     /translation="MAQIDVTNVTDVGTEPGTLPATMAAWVIRQEREGEPVDAFQVEE
                     IEVPEPGAFEVIVRVMAAGVNYNNVWAALGQPVSVFRYHPEDDHHIGGSDASGIVWKV
                     GAGVTRWKPGDEVVIHCNQASYEDPEVHGLDPLAAPSQKIWGYETSWGSFAQFTKVQA
                     QQLLPKPKNLTWEEAASYGLTYFTAYRMLIDQCEMQAGDKVLIWGAAGGLGVFAIQLC
                     KLTGAQCVGVVSSKEKGELVMQLGADGYIDRNEYKGMMRRGGETPDEEKARFKESRRF
                     GAAVKEILGDEPNIVFEHVGQATFPTSVLVVKPFGKVVICAGTTGYNLDFDVRYLWMR
                     QKQIIGSHFANAWECNKANELIEQGHIRPVLWRTMGFDGVAEAHQLMKENKHLGKIAI
                     LVGAEKEGLGKTEEGPGAIYAEVGA"
     gene            3895..4239
                     /locus_tag="VF752_07470"
     CDS             3895..4239
                     /locus_tag="VF752_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855426.1"
                     /translation="MIAEPGQPFVRIVWAVNPFRGDKFQAWGEPYAELALDFGATAWA
                     FVRSREDPLTFEQYAAFPTKLDFERYWYSEEAAARRVEATGLFQVPVLPEWFTTVGAG
                     AAVTVEELQRQE"
     gene            4245..4631
                     /locus_tag="VF752_07475"
     CDS             4245..4631
                     /locus_tag="VF752_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8855427.1"
                     /translation="MERLVITWVMNTVALFVAAWILSGVTYGDKWWTLLIAGLVFTVV
                     NRFVKPVLTVLSIPFIVVTLGIFYFLINILMLYLTDWLVPDFEIRSFGAGVLAAIIVS
                     LVNWVLHIVFGEPGEQRWGRRAAPRY"
BASE COUNT          739 a         1673 c         1658 g          842 t
ORIGIN      
        1 ctcggcgcgc tcgtgcacca catcatgcgc acgtgcagcg gtggggttct cgaggcggtc
       61 gacaagctcg gtctctcgct ctcgcagctc aaggcactca caacgctgac cggctcggag
      121 tcgctcgaga tgtcgctgaa agagatcggc gacgcgctcg gcctctccct ccccgcggtc
      181 agcagggcgg tcgacgggct ggtccagcgt gatctcgtca cgcgcaccga ggacccgctc
      241 gacaggcgca gcaagcgcgt cgccgccacg gccacgggcc gggcgctgct cgacgagctc
      301 accacgctcc gccttgtgga cctcgagcag ttcgtcgaga cgctgacccc cgagcagcgc
      361 gagctcctgg cgaacgccgt cacggcgatc acggctcgcg acgacatcaa gactctcccc
      421 cctcggaagg actgacctaa tgccagaacg gattcggctg acggaggaca accgcaagtg
      481 gtggacgctc gcggccatgt gcttcgcgct cttcatggtc atgctcgaca acacggtcgt
      541 gaacgtcgcg ctgccgtcga tccagaagga tctgggcgcg tcgatcacgt cgctcgagtg
      601 gaccgtcaac gcgtacacgc tcaccttcgc ggtcctcctc gtgacaggcg gtcgcctcgg
      661 cgacatcttc ggccgcaggc gcatgttcct gttcggcgtc gtgctgttcg cgctgtcgag
      721 cgctgcgatc ggattcgcgc ccaccgacac gctgctcgtc gccggccgcg cgattcaggg
      781 catcggtgcc gcgttcatga tgccgggcac cctctcgatc atctccaaca ccttcccgcc
      841 cgaggagcgc ggccgtgcga tcggcacgtg ggccggcgtc tcggcgctcg cgctcgcggt
      901 cggcccggtc gttggcggcg cactcaccga atacgtgtcc tggcgggcga tcttcttcct
      961 gaacctgccc gtggcggtcg gcgcggtcgc ggtcacgctg ttcgctgcgc gcgagtcgcg
     1021 cgacaacacg atcgagcgca aggtcgacat ccccggcatc gccgcgctat cggtgtccct
     1081 caccgcgctc gtgctcgcgc ttgtcgaggg caacggctgg ggctggacct cgggtcgcat
     1141 catcgcgctg ttcaccgtcg ccgccgtcgg cttcatcgcc ttcgcgctga ccgaaatgcg
     1201 cgtgcgcgtc ccgatggtgc agttcgcgtt cttcaagtcg cgctcgttcg ttggcgcgaa
     1261 cgtggtcgcg ttcatcgtct cgttcgcgat gctggcgatg ttcttcttca tcgccctcta
     1321 catgcagaac atcctgggct acagcccgct cgaggccggc gtgcgcttcc tgccctccac
     1381 ggcgatgatc atcatcgcgg cgccgatcgc tggccgcttg tcggaccgga tcgggccgaa
     1441 gcccctgatc gtcaccgggc tggggctcgc atcgttcgcg ctctacctgc aatcgacgat
     1501 cgacgtgaac acgggctacg gccacctgct gcccgcattc atcatcatgg gtatcggcat
     1561 gggcctgacc atgtcgccga tgtcgaccgc ggcgatgaac gcggtcagtg tcgacaaggc
     1621 gggcgcagcg tcgggcatcc tctcgatgag ccgcatggtg ggcggcacct tcggcgtcgc
     1681 tctgatcggc gcgctgttcg aatcgacctc gcgcaaccgg ctcgccgact cgctcggcaa
     1741 gctctcgctg acgccgggcc agcgctcctc gatcaccgat cagattgcga ccggcacagg
     1801 tggccagccc aagggcgtgc ctggcgcagt cgcgactcag gtgcacgccg cgtcgaacga
     1861 cgccttcatc catgcacttt cgagctccct caccgtggcg acgttcgtgg ccgccttcgg
     1921 cgcgatcgct gcggcggcgc tgatcgagag gaagcgcccc gcgcgcagcc gcgaacagga
     1981 gctcaggcaa agccccgagg ccgtggccgc cgaggaattg gcggcctgat cagaccggcc
     2041 tacctccccc acggggatcg ctagatgtcg attccgagtc ggcgtgtccg caggggcgag
     2101 cgccgaggag gaatcggcat ctagctccgg atctaggctg cgcggcgagt gttcgagacg
     2161 ccgcgcagcc agtccgctat cgactgcgcg ctcggctcga tcacggcatt cccggcactg
     2221 agcgcgcggc gcacgtggcc gtcggcaccg ccgtgcaggc tcacgtaggc gtccatgccg
     2281 tcgtgcatcg tcgaatcgtc gtccgcgccg aacggcggca cgaggatcgt cacgtcggcc
     2341 gccgagtcga ggtccacccc cgcgcgcgcc accgccgcgc tcacctgctc gagcacggcc
     2401 tccggcttgc cgtcggcctg cgtcgaggcg agcaggtaga ggcacgcgca gagtgggccc
     2461 tgccggcctg gctcgacgcc gaatcgatcg tcgccgccct gagtgccctg ccccccggcg
     2521 agctgccgtg gggtgacgtc tacgcgcgac tcccgggcgc ccggtagccc ccggcgccgc
     2581 gccttcgcgg ttcgccgggc ggtacgcttc ccctcctttt cgaccaggag gacgtaccga
     2641 ccagatggcg cagatcgacg tgacgaatgt gaccgacgtg ggcacagagc ccgggacgct
     2701 gcccgccacg atggccgcat gggtcatccg ccaggagcgc gagggcgagc cggtcgacgc
     2761 cttccaggta gaggagatcg aggtgccgga gcctggcgcc ttcgaggtga tcgtgcgggt
     2821 catggccgct ggcgtgaact acaacaacgt ctgggccgcg ctcggccagc cggtctccgt
     2881 cttccgctac caccccgagg acgaccacca catcggcggc tccgacgcgt ccggcatcgt
     2941 ctggaaggtc ggcgccggcg tgacccgctg gaagcccggc gacgaggtgg tgatccactg
     3001 caaccaggcg tcctacgagg atcccgaggt gcacgggctc gaccccctgg cggcgccgtc
     3061 gcagaagatc tggggctacg agacctcctg gggctcgttc gctcagttca ccaaggtgca
     3121 ggctcagcag ctgctgccga agccgaagaa cctcacgtgg gaggaggccg cgagctacgg
     3181 cctcacctac ttcaccgcct accgcatgct gatcgaccaa tgcgagatgc aggcgggcga
     3241 caaggtgctg atctggggag ccgccggcgg cctcggcgtg ttcgccatcc agctctgcaa
     3301 gctcaccggc gcgcagtgcg tgggcgtggt ctcctcgaag gagaagggcg agctcgtgat
     3361 gcagctcggc gctgacggct acatcgaccg caacgagtac aagggcatga tgcgcagggg
     3421 cggcgagacg cctgacgagg agaaggcgcg cttcaaggag tcgcggcgct tcggggcggc
     3481 ggtcaaggag atcctcggcg atgagccgaa catcgttttc gaacacgtcg gccaggcgac
     3541 gttcccgacc tccgtgctcg tggtgaagcc gttcggcaag gtcgtgatct gcgccggcac
     3601 gaccgggtac aacctcgact tcgacgtgcg ctacctctgg atgcggcaga agcagatcat
     3661 cggctcgcac ttcgccaatg cgtgggagtg caacaaggcg aacgagctga tcgagcaggg
     3721 ccacatccgc cctgtcctgt ggcgcacaat gggcttcgac ggcgtcgccg aggcgcacca
     3781 gctgatgaag gagaacaagc acctgggcaa gatcgcgatc ctcgtcggcg ccgagaagga
     3841 gggcctcggg aagacggagg aagggcccgg tgccatctac gcggaggtgg gcgcgtgatc
     3901 gccgagcccg gccagccgtt cgtccgcatc gtctgggcgg tcaacccgtt ccggggcgac
     3961 aagttccagg cctggggcga gccgtacgcc gagctggcgc tcgacttcgg cgcgaccgcg
     4021 tgggcgttcg tgcgctcgcg cgaggacccg ctcaccttcg agcagtacgc ggcgttcccg
     4081 acgaagctcg acttcgagcg ctactggtac tcggaggagg cggcggcccg ccgggtggag
     4141 gccacgggcc tgttccaggt gccagtgctg cccgagtggt tcacgaccgt cggcgcgggc
     4201 gccgcggtga cggtcgaaga gctgcagcgt caggagtagt tgccgtggag cggctggtca
     4261 tcacatgggt gatgaacacg gtcgctctct tcgtggcggc gtggatcctg tcgggcgtca
     4321 cgtacggcga caagtggtgg acgctgctga tcgcgggcct cgtgttcacg gtcgtgaacc
     4381 gcttcgtgaa accggtgctg accgtgctct ccatcccgtt catcgtggtc acgctcggca
     4441 tcttctactt cctgatcaac atcctgatgc tgtacctcac cgactggctc gtgcccgact
     4501 tcgagatccg ctcgtttggc gcgggcgtgc tggcggcgat catcgtcagc ctcgtgaact
     4561 gggtgctgca catcgtgttc ggcgagcccg gcgagcagcg ctggggccgc cgggcggccc
     4621 cgcggtacta gctcgggtcg gcggcgtcct tcagccgtgt ggcactgaga ggatcaggag
     4681 cgggaatttc ttccacccct gccggtctcg ccgcagcggt ggagattctt cccgctatgc
     4741 gggaatttct tccatcccaa cgtgcggccg gccgtcggga tggaagttct tcccgcctcg
     4801 ggtgttatgc ggtggtgcat ttcttccatc tctacggctt cccgtcgaag cgatggaagt
     4861 tatggaccgc ataggacccg tttcttccat cggaagcgcc acgacgggtc ga
//
LOCUS       DASVXS010000137        10405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1382256, whole genome shotgun sequence.
ACCESSION   DASVXS010000137 DASVXS010000000
VERSION     DASVXS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382256"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VF752_07480"
     CDS             complement(<1..1601)
                     /locus_tag="VF752_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX8855428.1"
                     /translation="MNGEYPAVEGMSFGRFLGVLWRRLWVIIACLVLVPAAALLYTRG
                     QEKQYSATAQVLLRDEGFEQNLAGNVTGDSSSSNQSPDTVFATNVGVAEVKAIAARTA
                     RAIDHGLTADRVAGEIAVDASNESNILSVKATDPDPQLAARIANVYSVQYIAFRRDLE
                     QRRVRTAQARIDRQIREVAVSRAKVENRLAGLRRQIRQSRPAIAGPARQGAARSESRQ
                     LTQLRQRASSVSDNLKALKQRESELRDRAGSLATLTALQTGDAVLVQRAEPPSSPSSP
                     NRARNLAAGIAIGLILGIVLAVLLELVDRRVRHPKEVETIFERPIIGTIPQNPALRRD
                     GLRLSPLYKEPFRMLQANLRYSNGDRPVRSVLVTSAGEGDGKSTVAWNIGAAGADVGV
                     RVLLIEADLRHSSFAARCGLEVQAGLTDVLSGRCDLAEVVHESNIGERGRGRNGSGDS
                     LTTPSMHIVFAGPPAPNPGGLLASRRMSKLLGEAELAYDLVVIDTPPTPVVSDAIPLI
                     KQVSGVIVVTRLGKNTREATAQLRD"
     gene            complement(1601..3136)
                     /locus_tag="VF752_07485"
     CDS             complement(1601..3136)
                     /locus_tag="VF752_07485"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8855429.1"
                     /translation="MFRTTRTGTSSQEEITTVSGVQPESSSATAGVTEPMTSNGRPAA
                     LPLPAARRASPPGFVLRRMLLAADALAITGALLLSVLLFGPERSLGDAAWGLLLLPLW
                     ILLFKVYGLYERDEKRVSHCTVDDVPWVFHALVVGSLGLWLFYRATPVDKLVFVEGAA
                     FFGFAFVGVLLARFVARELSRRANPPERVLMVGDGPVARLLMRKMRAHPEYALAPVGY
                     LDDGCVAGDGLAGEIPCLGKIPDFESVCERMGVERVVVAPTLEQEPLVDLVRRARGLS
                     LRLTLVPDVMDVLGPSVEIDDVEGITVLGINPPALARSSRMLKRGMDLAISSTALVFA
                     LVPMAVIAVAVRLTSPGPALFAQERVGRNGRTFRLYKFRTMSLDAEERTEELRALSSD
                     PDWVLVDDDPRITRVGRVLRRTSLDELPQLWNVIKGDMSLVGPRPLMSVDHAQVSEWG
                     LRRLDLTPGITGLWQVLGRTTIPFAEMVKLDYLYVTNWSLWNDTRLLIRTLPAVLRRR
                     GAN"
     gene            complement(3136..4602)
                     /locus_tag="VF752_07490"
     CDS             complement(3136..4602)
                     /locus_tag="VF752_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8855430.1"
                     /translation="MNEAVEGVLVAVRLLPATGSVRPDERGALSSFSGQLGLVVGASA
                     VAGVAMAATGDMRIPLGLLGFTTLAVLGFVRPAVFLGVFLATRPLLDAFSDKQFVHGL
                     TSANPAGVVAVLLVAMALVALSTSRRLFVPRGTTVLMLVLVLSVFSAAWALKDLRGVI
                     GTEPITELIRLSAFVAIYLLAANLFATPRRTQLVFTIVALSAVPPAVIGIAQWIHGLK
                     PEAGLTIGRVDSTFVGPNAFAAYLATAALLLIAATPQLPRRVRLPSLALILVALVGTY
                     SREGWVMFLLGILLLGWRRHRLVVVAAAAGAAALVFAVPAVHDRVIGSSGRVVSSSGV
                     RAAPTYESFDWRIGNWEKLLEKYRESPVVGFGLRTTPYVNPRRTEFLAPPSAGFEAHN
                     LAVRVLVEGGPVLLLAYIALFAAMVGEAIRLARARWPLQRLGTVVLVLWVLAIVVGLT
                     TDDPLSNTTMTFALFALAGSLEGARRTWLAQAAVAEGR"
     gene            4612..6039
                     /locus_tag="VF752_07495"
     CDS             4612..6039
                     /locus_tag="VF752_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEX8855431.1"
                     /translation="MLFTSAAYLLLFLPATVAAYWLVPARLRVVLLLVASYVFYLSWN
                     PKYGLLLAASTVVNFTLAKRLPSARSPRALLALGVAFNLGVLAYFKYVGLFSKSAADL
                     IDWLGLGRTGSFDGLHVLLPLAISFFTFEMISVLVDVYRGDVRVGGFLTFAAYKAFFP
                     KLVSGPITRYRELAPQIEKPQPLRFERFQSGVALFALGLAKKLVLADNVAVLANTLFN
                     DPAAANSGTAAVGILAFGVQIYLDFSAYTDMARGAARMLGLELPRNFNFPYAATSPSD
                     FWRRWHMSLSRWLRDYLYIPLGGNRHGRLNTYRNLMATMVLGGLWHGAGWHFALWGAM
                     QGVFLSVDHALRGRFPRPSRALTLTARGATLVAVFAAWVFFRAQSLGQAFDVFAALGR
                     LPFDLAAPTALYSSPPVGIVLAALLAFVLGSAPAARLSPRLRALVLPRPTVRLAALAS
                     LTVCCWTLTDVLTRSTTVPFIYFRF"
     gene            6036..7121
                     /locus_tag="VF752_07500"
     CDS             6036..7121
                     /locus_tag="VF752_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855432.1"
                     /translation="MTRARTALTCLVYIVLLVAVAVGIDRAISDRLFAHPNDYVPAYR
                     SFQEYAVGVKLHEFESQHRRFSGLFLGNSRTMFGVDPPVVDAELGRRGVDFSSFNLAL
                     PSVDTRFWPPFFERYYDEPPPRQLFLGLLPRDLDASFSLGAREVQAFLHSAGFQNRNM
                     SAVSSWAEESLARLFVLRGRLADTRLITLSDLLEHKKLDLNAIHLANDRGWAELPRGT
                     MLPKSELAAQAQKLAVRHGSARFELGAEQRKSLVLLNSWIRSRGGCLTLFTTPLLFDA
                     EPWGTIEMRRGFLRAMRRLVREIPGLRFVDIGARVQGGYGTADFADGDHLAPAGATRF
                     SRQLADALLPSITDPGCQRPAIVARRP"
     gene            7124..8746
                     /locus_tag="VF752_07505"
     CDS             7124..8746
                     /locus_tag="VF752_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8855433.1"
                     /translation="MDDLAATAVPAREATAPSTPRYPGLAALPDGKVRSAAADAAVYT
                     AGVYGAQLLLFVAGVVQKGLLGPTATGYWALMATFYAFFNIAPLGAFDGAVRQIPFHR
                     GRDDRRGAAALADTASSFTVLAVTVASLLVSSAAVAFGSSWPAEIRYGLILLAFGAPL
                     RFLIDCHESVLQATKRFDVVSLGLISRAVIAVTLQTLFVLLWGYWGMFAGVTAMAVGQ
                     LLLWNRMGLTSWSRPAFRRRIEGRRLKELIAFGFPMMIHAQIWLLFLAIDNLIVARFL
                     DVTQLGYYALAVSVTTYIMLLPKGIAGALAPRMAERFGRTGDVSSIRDYATDVQQLLA
                     YLLIPVLVAAAFFLMPVLIRHALPEFMPAIPVVRITVAASFFISLTNMPIKVMLTSGR
                     NWRLVSLMLACLAFNGLANYLAVAVLDRGLEGAAFATALSYFVLFVVVSSFALRGPLG
                     TRGVTGHIAELVVVFVYVSAALWGIEWIVGPGGGGLGSDLLTGTAEFALFLVTLAPWA
                     ALVERRYKGLSAARAALTDAARALRRWRLRNG"
     gene            8739..9581
                     /locus_tag="VF752_07510"
     CDS             8739..9581
                     /locus_tag="VF752_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX8855434.1"
                     /translation="MADRRTTIIAEIGENHAGDIELARRMVAEAARAGADVVKFQSYR
                     GADVAPDDPEREWFTQVELSDRAHRDLAELARSFGVAFMSTPFSLERARFLVEELGLT
                     AIKVASSEMLNFALLDYLAERVETVFLSTGMADLDEVRLAVARLEGVEHVVIMHCVTQ
                     YPLADDHANLRAISTLAEAFPGRSVGYSDHTIGLLAPLLAVALGATAIEKHFTLDRSL
                     PGTDHVLSLTPDELAALVRTVRRSEALLGREGKQPAEPELEIREFVRGRFPKPAPSGT
                     AGGQ"
     gene            9578..10276
                     /locus_tag="VF752_07515"
     CDS             9578..10276
                     /locus_tag="VF752_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEX8855435.1"
                     /translation="MRVAAFVQARMGSTRLPGKALLPVWRDMPLIELVLRRASAAASL
                     DNVVLVTTSDERDDPLAEVGERLGVAVFRGAEHDVLGRFASALERHPADAVVRVCADN
                     PFVDPSALDQLVGFFESVQPCDYASNHTALSGLPDGSGGEVMSATSLRRAAAEAVSPY
                     DREHVAPFVVGRPEAFRIRFAPPPADPWPFLKLDIDTPRDYSRLRRLADRLPDDQAPL
                     WDLPTVVECATPSS"
BASE COUNT         1482 a         3748 c         3511 g         1664 t
ORIGIN      
        1 tggtcccgga gctgggctgt cgcctcgcga gtgttcttgc cgaggcgggt gaccacgatc
       61 accccgctca cctgcttgat cagcgggatc gcgtccgaga cgaccggcgt cggcggcgtg
      121 tcgatcacca caaggtcgta ggcgagctcc gcctcgccga gcagcttgct catccggcgc
      181 gaggcgagca atccgccggg gttcggcgcc ggcgggccgg cgaacacgat gtgcatgctg
      241 ggcgtcgtca gcgagtcgcc ggagccgttc cgcccgcggc cccgctcccc gatattgctc
      301 tcgtgcacca cctcagcgag gtcacagcgg ccagagagga catcggtcaa gcccgcctgc
      361 acctcgaggc cgcaccgtgc ggcgaagctc gagtggcgga ggtccgcctc gatcaggagc
      421 accctgaccc ccacgtccgc gccggcagcc ccgatgttcc acgcgaccgt gctcttgccg
      481 tcgccctcgc ccgcggaggt cacgagcacc gatcgcaccg gccggtcccc gttcgagtag
      541 cgcaggttcg cctggagcat tctgaacggc tccttgtaca gcggcgagag ccgcagcccg
      601 tcgcgccgga gcgcggggtt ctgcgggatc gtcccgatga tggggcgctc gaagatcgtc
      661 tcgacctcct tcggatgccg cacgcgccgg tcgaccaact cgagcagcac cgcgagcacg
      721 atcccgagga tcagcccgat cgcgatgccg gccgcgaggt tccgggcgcg gttcggcgag
      781 ctcggcgacg acggcggctc cgcgcgctgc acgagcacgg cgtcgccagt ctggagcgcg
      841 gtcagcgtgg ccaggctccc ggcccggtcg cggagttccg actcccgctg cttgagcgcc
      901 ttcagattgt cggagacgct gctcgcccgc tggcgcagct gggtcagctg ccgggactcc
      961 gagcgcgccg ccccctggcg cgcgggcccg gcgatcgccg ggcggctctg ccggatctgg
     1021 cgtcgcagcc cggcgaggcg gttctccacc ttcgcgcggc tcaccgcgac ctcgcggatc
     1081 tggcggtcga tgcgcgcctg cgcggtccgc acgcgccgct gctcgaggtc gcgcctgaat
     1141 gcgatgtact gcacgctgta gacgttcgcg atccgggccg cgagctgcgg gtcggggtcg
     1201 gtcgccttaa ccgagaggat gttcgactcg ttgctcgcgt cgaccgcgat ctcgcccgcc
     1261 acgcggtcgg cggtgagacc gtgatcgatg gcgcgagcag tacgcgcggc gatcgccttc
     1321 acctcggcca ctcccacgtt ggtcgcgaac accgtgtcgg gcgactggtt cgaggacgac
     1381 gagtcgcccg tcacgttgcc cgcgaggttc tgctcgaagc cctcgtcacg cagcagcacc
     1441 tgagcagtgg ccgagtactg cttctcctgt ccgcgcgtat agagcagcgc ggccgctggg
     1501 acgagcacca ggcaggcgat gatcacccac aggcgccgcc agagcacgcc gaggaagcgg
     1561 ccgaagctca tcccttcgac ggcgggatat tccccgttca tcagttcgct ccccggcgcc
     1621 ggagaaccgc agggagcgtg cggatcagca gacgcgtgtc attccacagc gaccagttgg
     1681 tcacgtacag gtagtcgagc ttgaccatct cggcgaacgg gatcgtggtg cggcccagca
     1741 cctgccagag cccggttatg ccgggcgtca ggtcgaggcg ccgaagcccc cactcgctca
     1801 cctgggcgtg atcgaccgac atcagcggcc gcggtcccac caagctcatg tctcccttga
     1861 tcacgttcca gagctggggc agctcgtcga ggctcgttcg ccggagcacc cgaccgacgc
     1921 gggtgatgcg cgggtcgtcg tccaccagga cccagtcggg atccgagctg agcgcgcgga
     1981 gctcctccgt ccgctcctcc gcgtcgaggc tcatcgtgcg gaacttgtag aggcggaagg
     2041 tgcgtccgtt ccgtcccacg cgctcttgcg cgaacagtgc cggcccgggc gacgtcaggc
     2101 ggaccgccac ggcgatcacg gccatcggga cgagcgcaaa gacgagcgcc gtggacgaga
     2161 tcgccaggtc cattccccgc ttcagcatcc gcgacgagcg ggcgagcgcc ggcgggttga
     2221 tgccgagcac ggtgatcccc tcgacgtcgt cgatctcgac cgacggaccg agcacatcca
     2281 tcacgtccgg tacgagcgtc agccgcaggc ttagtccgcg cgcgcgccga acgaggtcca
     2341 cgagcggctc ctgctcgagc gtcggcgcga caaccacacg ctccacaccc atccgctcgc
     2401 acacggactc gaaatcggga atcttcccga ggcacgggat ctcgccggcc agcccgtccc
     2461 cggcgacgca gccgtcgtcg agatagccga cgggagcgag ggcgtactcc ggatgcgcgc
     2521 gcatcttgcg catcagcaac cgggcgaccg ggccgtctcc caccatcagc acgcgttcgg
     2581 gcgggttcgc ccttcgcgag agctcgcgcg cgacgaagcg tgcgagcagc acgccgacga
     2641 acgcgaaccc gaagaacgct gccccctcga cgaagacgag cttgtcgacc ggcgttgccc
     2701 ggtagaacag ccaaagtcct agcgaaccca ccacgagcgc gtggaagacc cacggcacgt
     2761 cgtcgacggt gcagtggctg acgcgcttct cgtcgcgctc gtagagcccg tagaccttga
     2821 acagcagaat ccagagcggc agcaggagca gcccccaggc ggcatcgccg aggctgcgct
     2881 ccggcccgaa caacaggacg ctgagcagga gcgctcccgt tatggcgagc gcgtcggcgg
     2941 cgagcaacat ccggcggagc acgaaccccg ggggcgatgc acggcgcgcg gcgggtagcg
     3001 gcagcgcggc cggccggcca ttcgaggtca tcggttccgt gacgccggcg gtcgcggaag
     3061 agctctccgg ctggacgccc gatacggtcg tgatctcctc ctgcgagctg gtccccgttc
     3121 gcgtagtccg gaacatcaac gcccctccgc gaccgccgct tgcgcaagcc aggtacgccg
     3181 cgccccctcc aggcttccgg cgagcgcgaa cagcgcgaac gtcatcgtgg tgttggaaag
     3241 cggatcgtcg gtggtgagtc ccactacgat cgccagcacc cagaggacga gcacgacggt
     3301 gccgaggcgc tgcagcggcc agcgggcgcg cgccagccgg atcgcctcac cgaccatcgc
     3361 ggcgaacagc gcgatgtagg cgagcagcag cacggggccg ccttccacga gcaccctcac
     3421 tgccaggttg tgcgcctcga aacccgcgct tggcggggcg aggaactccg tccggcgcgg
     3481 attcacgtac ggggtcgtgc gcaatccgaa gccgactacc gggctctccc ggtacttctc
     3541 cagcagcttc tcccagttgc cgatgcgcca gtcgaacgac tcgtacgtgg gtgcggcgcg
     3601 taccccgctg gaagagacca cgcggccgct cgagccgatc acgcgatcgt ggaccgcagg
     3661 caccgcgaac acgagagccg cggcgccggc cgccgcggcg accacgacca ggcggtgccg
     3721 tcgccagccg agcagcaaga tgccgaggag gaacatcacc cagccctcgc ggctgtaggt
     3781 cccgaccagc gccacgagga tgagtgcgag gctcggcaac cggacccggc ggggcagctg
     3841 cggtgtggcg gcgatcagca gcagggccgc cgtcgcgagg taggccgcga acgcgttcgg
     3901 cccgacgaac gtgctgtcca cgcgaccgat ggtgagcccg gcctcgggct tgagtccgtg
     3961 gatccattgg gcgatcccga tcacggccgg cggaaccgca ctgagcgcga cgatggtgaa
     4021 cacgagctgc gtgcggcgtg gggtcgcgaa caggttcgcc gcgagcagat agatcgccac
     4081 gaacgccgag agccggatca gctcggtgat cggctcggtc ccgatcacgc cgcggagatc
     4141 cttgagcgcc caggcggcgg agaacacgct caggacgagc acgagcatga ggaccgtcgt
     4201 cccgcggggc acgaacagcc ttcgcgacgt gctgagcgcg accagggcca tcgcaacgag
     4261 cagcaccgcg accacgccgg ccggattggc cgaggtgagc ccgtggacga actgcttgtc
     4321 gctgaaggcg tcgagaaggg gtcgagtggc caggaagacc ccgaggaaaa cggcggggcg
     4381 gacgaagccg aggacggcga gagtcgtgaa cccgagcagg ccgagcggga tcctcatgtc
     4441 gccggtcgcg gccatcgcca cgccggccac cgcgctcgcg ccaaccacca gcccgagctg
     4501 ccccgagaac gagctcagcg cgccccgctc atccgggcgc accgagcctg ttgcgggcag
     4561 gagcctcacc gccacaagaa cgccctcgac ggcctcgttc attcggtagc cgtgctcttc
     4621 acgtcggcgg cgtatctact gctgttcctc ccggcgacgg tcgccgccta ctggctcgtg
     4681 ccagcgcgcc tgcgagtcgt cctgctgctc gtggcgagct acgtcttcta cctcagctgg
     4741 aatccgaagt acgggctgct gctcgcggcg agcaccgtcg tgaacttcac gctcgccaag
     4801 cggctgccgt cagctcgttc gccgcgggcg ctcctggcgc tgggcgtggc cttcaacctc
     4861 ggcgtgctcg cctacttcaa gtacgtcggg ctcttctcga agtccgccgc ggacctgatc
     4921 gactggctcg gtctcggccg caccgggtcg ttcgacggcc tccacgtgct gctgccgcta
     4981 gcgatcagct tcttcacctt cgagatgatc agcgtgctcg tcgacgtcta ccgcggcgac
     5041 gtgcgagtcg gcggcttcct caccttcgcg gcgtacaagg ccttctttcc gaagcttgtc
     5101 tcgggcccaa tcacccgcta ccgcgagctc gcgccgcaga tcgagaagcc gcagccgctc
     5161 agattcgagc gcttccagtc cggcgtggcc ctgttcgcgc tcggactcgc gaagaagctc
     5221 gtgctggccg acaacgtggc cgtcctcgcg aacacgctgt tcaacgaccc tgccgctgcc
     5281 aactcgggca ccgccgcggt cgggatcctc gcgttcggcg tccagatcta cctggatttc
     5341 tcggcgtaca ccgacatggc tcgcggcgcc gcgcgcatgc tcggcctcga gcttccgcgc
     5401 aacttcaact tcccctatgc ggccacgagc ccgtccgact tctggcggcg ctggcacatg
     5461 tcgctgtcgc gctggctgcg cgactatctc tacatcccgc tcggcggcaa tcgccacggc
     5521 cgcctcaaca cctatcgcaa cctcatggcg acgatggtgc tcggcgggct ctggcacggc
     5581 gcgggctggc atttcgcgct ctggggagcg atgcaagggg tattcctctc ggtcgaccac
     5641 gcgctgcgcg ggcgcttccc gaggccgagc cgcgcactga cgctcaccgc gcgcggcgcc
     5701 accctcgtcg ccgtcttcgc ggcgtgggtg ttcttccgcg cgcagtcgct cggacaagcc
     5761 ttcgacgtgt tcgccgcgct cggccgactc ccgttcgacc tcgcggcccc gaccgcgctc
     5821 tactcgtcgc cacccgtcgg gatcgtgctg gcggcgctgc tggcgttcgt gctcgggagc
     5881 gcgcccgcgg cccgcttgtc gccccgcctc cgggcactcg tgctcccccg gccgacggtc
     5941 cgcttggcgg cgctcgcgtc gctcacggtc tgctgctgga cgctcaccga cgtgctcacc
     6001 cggtccacca ccgtcccgtt catctacttc cgcttttgac tcgcgctcgc acagctctca
     6061 cctgcctcgt ctacatcgtg ctgctcgtgg ccgtcgccgt cggcatcgac cgggcgatct
     6121 ccgaccggct gttcgcccac ccgaacgact acgtgccggc ctaccgctcg ttccaggagt
     6181 acgccgtcgg cgtgaagctg cacgagttcg agtcgcagca ccggcgcttc agcgggctgt
     6241 tcctcggcaa ctcgcgcacg atgttcggag tcgacccgcc agtggtcgat gccgagctcg
     6301 gaaggagggg cgtcgacttc agctccttca acctggcgct gccgagcgtg gacacccgct
     6361 tctggccgcc gttcttcgag cgctattacg acgagccgcc gccgcgccag ctcttcctcg
     6421 gtctgctgcc gcgagacctc gacgcgagct tctcgctcgg cgcccgcgag gtccaggcgt
     6481 ttctgcacag cgccggcttc cagaaccgca acatgagcgc ggtctcgagc tgggcggagg
     6541 agtcgcttgc ccggctgttc gtcctccgcg ggcggctcgc cgacacgcgg ctcatcacgc
     6601 tctccgatct cctggagcac aagaagctcg acctgaacgc gatccacctc gccaacgacc
     6661 gcggctgggc ggagctgccc cgggggacca tgctccccaa gtccgaactg gccgcccagg
     6721 cccagaagct ggcggtccgg cacgggagcg cccgctttga gctcggcgcc gagcagcgta
     6781 agagcctggt gctgctcaac tcgtggatcc gcagccgtgg cggctgcctg acgctcttca
     6841 ccacgccgct gctgttcgac gcggagccat ggggcacgat cgagatgcga cgcggcttcc
     6901 tgcgcgcgat gcgccgcctg gtgcgggaga tccccgggct gcgcttcgtc gacatcggtg
     6961 cgcgcgttca gggcggctac ggaacggccg acttcgcgga cggcgaccac ctggcgccgg
     7021 ccggcgcgac gcgcttctcg cgtcagctcg ccgacgccct cctgccatcg atcaccgatc
     7081 ccggctgcca gcggcctgcg atcgtcgcga ggcgcccgta gcgatggacg atctggcagc
     7141 caccgctgtg ccggctcgcg aggcgacggc gccgagcacg ccgcgctacc ccgggctggc
     7201 cgcgctgcca gacggcaagg tgcgctcggc cgccgccgac gcggccgtct acacggccgg
     7261 cgtgtacggc gcgcagctcc tgctcttcgt cgccggcgtg gtgcagaagg gcctgctggg
     7321 cccgactgcg accggctatt gggcgctgat ggcgaccttc tacgcgttct tcaacatcgc
     7381 cccgctcggg gcgttcgacg gcgcagtccg ccagatcccc ttccatcgtg gccgcgacga
     7441 ccgccgcggc gcggcggccc tcgcggacac cgccagctct ttcaccgtcc ttgccgtgac
     7501 ggtcgccagc ctgctcgtct cgtccgccgc cgttgctttc ggatcctcgt ggccggctga
     7561 gatccgctac ggactgatcc tgcttgcctt cggcgcgccg ttgcgcttcc tgatcgactg
     7621 ccacgagagc gtgcttcagg cgaccaagcg cttcgacgtc gtgtcgctcg gcttgatctc
     7681 gcgggcggtg atcgcggtca ccctgcagac gctgttcgtc ctgttgtggg gttactgggg
     7741 catgttcgcg ggcgtaacgg cgatggccgt cggccagctt cttctgtgga accgaatggg
     7801 actgacgagc tggtcgcgcc cggcgtttcg ccggcggatc gaggggaggc ggctgaagga
     7861 gctgatcgcg ttcggcttcc cgatgatgat ccacgcccag atctggctgc tgttcctggc
     7921 aatcgacaat ctgatcgtcg cccgcttcct tgacgtcact cagctcggtt actacgcgct
     7981 cgccgtctcg gtgaccacgt acatcatgct gttgcccaag ggcatcgccg gtgcacttgc
     8041 gccacggatg gccgagcgct tcggacgcac cggagatgtg agctcgatcc gcgactacgc
     8101 gacggacgtc cagcaactgc tcgcgtactt gctcattcca gttctcgtgg cggcagcgtt
     8161 cttcctcatg ccggtgctga tccggcacgc gctgccggag ttcatgcccg ccattccggt
     8221 cgtgaggatc accgtggccg cgagcttctt catctcgctc accaatatgc cgatcaaggt
     8281 gatgctgacg agcgggcgca actggcgtct cgtgtcgctc atgctcgcct gcctggcgtt
     8341 caacgggctg gcgaactacc tcgcggtggc cgtgctggac cggggcctcg agggtgcggc
     8401 gttcgccacc gcattgagct acttcgtgct gttcgtggtc gtcagcagct tcgcgcttcg
     8461 tggaccgctc ggcacgcgcg gcgttaccgg ccacatcgcc gagctcgtgg tggttttcgt
     8521 ctacgtgagc gcggcgctct ggggcatcga atggatcgtc gggccgggcg gcggcggtct
     8581 gggctcggat ctcctgaccg gcacggccga gttcgcgctg ttcctggtga cgctcgcccc
     8641 ctgggccgcg ctcgtcgagc gccgctacaa gggtctcagc gcggcgcgag ccgccctgac
     8701 cgacgcggcg cgggcgctgc gtcgctggag gctgcgcaat ggctgaccgc cgcacgacga
     8761 tcatcgccga gatcggcgag aaccacgccg gggacatcga gctcgcgcgc cggatggtgg
     8821 ccgaggcggc tcgggcgggc gcggacgtcg tgaagttcca gtcctatcgc ggcgcggacg
     8881 tcgctccgga cgacccggag cgcgagtggt tcacgcaggt ggagctctcc gaccgcgcgc
     8941 accgggacct ggcagagctg gcgcgctctt tcggcgtcgc gttcatgagc actccgttct
     9001 cgctcgagcg ggcgcgcttc ctcgtcgagg agctcggtct cacggcgatc aaggtcgcct
     9061 cctcggagat gctgaacttt gcgctgctcg actacctcgc cgagcgagtc gagaccgtct
     9121 tcctttcgac cggcatggcg gacctggacg aggtgcgcct tgcggtcgcg cgcctcgaag
     9181 gggtcgagca cgtggtgatc atgcactgcg tcacgcagta cccgctcgcc gacgaccacg
     9241 ccaatctgcg cgcgatctcg acgctggccg aggcattccc cggccggagc gtcggctatt
     9301 ccgaccacac gatcggcctg cttgcccctc tgctcgcggt cgcgctcggc gccacggcga
     9361 tcgagaagca cttcacgctc gaccgctcgc tgccgggcac cgaccacgtg ctgtcgctga
     9421 ccccggacga gctggcggcg ctggtgcgca ccgtgcgccg gtcggaggcg ctcctcggcc
     9481 gcgagggcaa gcagccggca gagcctgagc tcgagattcg cgagttcgtg cgcgggcgct
     9541 ttcccaagcc cgcgcccagc gggacggcag gtggccagtg agggtcgcgg cgttcgtaca
     9601 ggcccgcatg ggctcgacgc gcctcccggg gaaggcgctg ctgccggtgt ggcgggacat
     9661 gcccctgatc gagctcgtgc ttcgccgcgc gtccgccgcc gcgagcctcg acaacgtggt
     9721 cctcgtgacc acctcggacg agcgcgacga cccgctggcc gaggtcggcg aacgcctggg
     9781 cgttgccgtg ttcagaggcg ccgagcacga tgtgctcggc aggttcgcgt ccgcgctcga
     9841 gcgtcatccc gcggacgccg tggtgcgcgt gtgcgcggac aatccgttcg tcgacccgag
     9901 cgcgctcgac cagcttgtgg gcttcttcga atcggttcag ccctgcgact acgcgagcaa
     9961 ccacaccgcg ctgagcggcc tccccgacgg aagcggcggc gaggtgatgt cggcgacttc
    10021 gctgcgacga gccgccgcgg aggcggtgtc gccgtacgac cgcgagcacg tggcaccgtt
    10081 cgtggtcggg cgccccgagg cattccggat caggttcgcg ccgccgcctg ctgatccgtg
    10141 gccgttcctg aagctcgaca tcgacacgcc ccgcgactac tcgcgtctgc ggcggcttgc
    10201 cgacaggctg cccgacgatc aggcgccgct ctgggatctg ccgacggtgg tcgagtgcgc
    10261 tacgccgagc tcctgaggta gctcgacgcc ggcgtcgtcc cttgtgcgcg tgccgatcaa
    10321 gcgcatcagc gcctcggtcg gatcgggacc ttccacgcgt agcgcgccca gaaggtcgtc
    10381 ccggcccgga agagcctcgc cggct
//
LOCUS       DASVXS010000138        11257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_179831, whole genome shotgun sequence.
ACCESSION   DASVXS010000138 DASVXS010000000
VERSION     DASVXS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179831"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF752_07520"
     CDS             complement(<1..326)
                     /locus_tag="VF752_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855436.1"
                     /translation="MTSPPSSAVSVVTALFEDLVRVGLRQLITQDPNLELVASDVPLE
                     QLDEAIDELEPQVVLLNFGSLPSPGYVHDLHEAHPGSRIVVLANRPTSAECNQMLSFG
                     ATACIS"
     gene            672..1358
                     /gene="rpiA"
                     /locus_tag="VF752_07525"
     CDS             672..1358
                     /gene="rpiA"
                     /locus_tag="VF752_07525"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX8855437.1"
                     /translation="MTPAIDEQSRAREAAARAAVDRIQAGQTLGFGSGRAVWKVIETL
                     GERAPERAVAASTRTEERLREAGIEPLSIDDAGTVDLVIDGADELDHSLNLIKGGGGA
                     HLREKLLVEAGRDFLVVAETPKLVDRLGTNWPVPVEIVRFGWVTTRARLLEICASAER
                     RTDDRGQAYVTDEGNWILDCRIPADGDLEELAAAIKSETGVIEHGLFLGMASAAILGT
                     PGGDVQTLSA"
     gene            1426..1659
                     /locus_tag="VF752_07530"
     CDS             1426..1659
                     /locus_tag="VF752_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855438.1"
                     /translation="MTEATKLAEKVSSKDPAVGLKAVASLRTLLERLERLQVENAREQ
                     GWSWQQIAAALDVSKQAVHKKHGGGGLLSRRRK"
     gene            1660..2133
                     /locus_tag="VF752_07535"
     CDS             1660..2133
                     /locus_tag="VF752_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX8855439.1"
                     /translation="MFTRFVQSARTAVVEAQAATRALGHDSVTPSHMLVGVARAEPEL
                     TSPLTAEQLQSAIRAPGIDRRALASIGIDYDAVEREVEASFGPGALASGRTCGQLAFA
                     PESKKALELALREAQDREDEHIGPEHLLLALLDDHDVAEVIRRCGADPKEMKDRL"
     gene            complement(2130..3035)
                     /locus_tag="VF752_07540"
     CDS             complement(2130..3035)
                     /locus_tag="VF752_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEX8855440.1"
                     /translation="MKVAYWPGCVSRGFTPELHGSMAAVAPKLDIELVELDRANCCGA
                     GVIAEHNQELVDSLNARTFALAQSVEGAAGMMNICSTCQGAQTECQERLDASDEYRDH
                     VNSHLAPEGLSYQRNGDWWNKNFLWLLVEEIGLDSLRERVVRPLDGLRVAPFYGCYIV
                     RPSNRLGLNERPERRLYLEMVIEALGGEPVDYAGKQKCCGFPLITMNKETSLRQAGRH
                     IADAIDADADCMVTPCPLCHLNLDLQQPDAAKRVERDLGLPVLHFPQLVGLALGLSPK
                     ELGMNKHVIGTDDVQRIVAGVAAAA"
     gene            complement(3032..4132)
                     /locus_tag="VF752_07545"
     CDS             complement(3032..4132)
                     /locus_tag="VF752_07545"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX8855441.1"
                     /translation="MAEYTLKIRRFDPESGQAAYWQDFQVDLPPERSVLDGVLQARDR
                     IDGSISIRCSCQAAICGSCAVRINGRTGLACNTKIGEAADRAVTDDGAIVVEPMSNMP
                     VLKDLIVDMDAVHWKKVQRIVPWLLPAGPPPEREYIVPAESMIDVTQAMACIHCGACV
                     GACLSLEVDSEFIGPAALAKAYRFVGDPRDGATEERLTDLAEDPHGIYDCTHCFACVE
                     VCPKGVAPMDQIMRLRRRATGDYGIKDRNNGYGHEKAFVDLIQKYGTLHEAQLLPRSY
                     GNGSFVKAALHPSGIKQLTESLPTVMNGVKSGKVAPAKALHHPTLPDQKQVRRIYKEI
                     ESKDERVELNLYIVGESGGEEEGTMAEGGRES"
     gene            complement(4152..5834)
                     /locus_tag="VF752_07550"
     CDS             complement(4152..5834)
                     /locus_tag="VF752_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8855442.1"
                     /translation="MPQHDVLIIGAGLAGQRAALAAAHGGASVGIMSKVHPVRSHSNA
                     AQGGINAALNPEDSWESHAFDTVKGSDYLGDQDAIEIMCREAPNEILDLEHLGVTFHR
                     NEEGRLGTRAFGGASAARTYYVADITGQAILHVLYEQLMKHNEVYRYEEWFVTSLMRD
                     GNGRISGAVARNIRDGSMELFVAKSVILATGGNGQIYNPTTNALICTGDGIAMAYRVG
                     AKLMDMEMVQYHPTTLQGNGLLITEGARGEGAHLYNALGERFMEKYAPNKMELASRDV
                     VSRAEQTEINEGRGFPDGTVALDITKVPRKRIHEALREIVMVGRDFAGVDITREPIHV
                     KPGNHYTMGGVDTDIDGQTNIPGLYAAGECACVSVHGGNRLGANSLLDTLVFGRRSGE
                     HAARTGQTLPMPKPDHSWVETEERNIQEIVGRERTGRRTSEIKAEMGATMDKYAAVFR
                     DEDGLRTALGIVDRLKDEAKTVATDDKGTVFNQDVLTALELGFMLDNAECVVTAAIER
                     RESRGAQFRTDYPERNDDEWLKHIDLSLNGDRPEISYSDVTITQWQPEERRY"
     gene            complement(5943..7106)
                     /locus_tag="VF752_07555"
     CDS             complement(5943..7106)
                     /locus_tag="VF752_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8855443.1"
                     /translation="MFGGRSLQLARVFGIRIGVNPSWFFILFLLIWSLSASYKDVTGS
                     DSTAFALAVASALLFFGSILLHELGHAFVAVRNGIEIEGIELWLFGGMARMRREAPTA
                     GAEFKVAVAGPIVTLLVVVACTALGIAIGGADEFKDAVRFSGSADISPGVLLLGWLAE
                     VNAFLFVLNILPGFPLDGGRMVRAIAWWRTGDRLRATRIAARLGVALAYLLGALGVFL
                     FLQGYLIEGVWDIFIAFFIYQAARVEEAQTGLSTRLKSVSVADVMDDEPVSIPASLPL
                     DRAYDDYFLRYGWQWFPVVDENGRYVGVLDRDRVDQVPHDTRQGWQVREAVIGSRDDD
                     LQVPVDESLDALLGSEGLQRLGALMAVDRDGVLRGVVTLDRVRAALRASIPAA"
     gene            7210..8304
                     /locus_tag="VF752_07560"
     CDS             7210..8304
                     /locus_tag="VF752_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX8855444.1"
                     /translation="MAYRVTFIPGDGTGPEISEAVQRVLEATGVDFEWDFQDAGVDVY
                     EAEGTPLPDRVIESIRERGLAIKGPITTPVGSGFRSVNVALRKELDLYACLRPCKAYE
                     GVRTRFPETDIVIVRENHEDLYAGVEYEIGDPNTEKLRQFIKDTEGTELREDIGVSIK
                     PISVYGTQRIVREAFEYAKANGRSKVTLAHKANIMKHTDGLFLRVGREVAEREYPDVE
                     FEDRIIDNLCNQLVSRPEEYDVIVLPNLYGDIVSDLGAGMIGGLGLAPGANVGDDYAV
                     FEAVHGSAPKYKGQNKVNPMALMLSGVMLLRHIDEREAAERMEAAIAEVIREGKSVTY
                     DMKPTRDDPTAVGTSDVATAIIEKMEKVAV"
     gene            complement(8321..8698)
                     /locus_tag="VF752_07565"
     CDS             complement(8321..8698)
                     /locus_tag="VF752_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX8855445.1"
                     /translation="MADFLAEKRAEIETRLKELRPLHDEYLKLERAKAALEGVDAPRR
                     GPGRPRTSGTSSNGRRRRGRPRGTGNRAQQALAIVRQNPGIRVSDIARELGVSPPNYM
                     YRVMANLESEGAVKKQGRGYTAA"
     gene            8759..9688
                     /gene="mdh"
                     /locus_tag="VF752_07570"
     CDS             8759..9688
                     /gene="mdh"
                     /locus_tag="VF752_07570"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX8855446.1"
                     /translation="MRRKITVVGAGNVGATTAQRLAERDYADVVVVDIVEGMPQGKAL
                     DLNQSGPVVGYEPNVVGTNGYDETAGSDIVVITSGFPRQPGMSRDDLLSKNKEIVGGV
                     AAQVAERSPDAIIVVVTNPLDAMCHVALDVSGFPRQRVIGMAGILDSARFRTFLAWEL
                     GVSARDVTGFVLGGHGDTMVPVVSYTNVAGVPVRQKISDGRLEEIVQRTRDGGAEVVK
                     LLQKGSAFYAPSAAVAEMVDSIVFDQKRVLPCAALCQGEFGIDGLFVGVPVRLGAEGV
                     EEIVEIELDDAERGELEKSADAVRELVDAMKAL"
     gene            complement(9705..9995)
                     /locus_tag="VF752_07575"
     CDS             complement(9705..9995)
                     /locus_tag="VF752_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855447.1"
                     /translation="MSDSIGTRAAGGWSPFARPPWRDCEAAAKAVLRARDIDGCALNE
                     LSEDRYVAIRDRLPGAGLPRITTLTRACGSWAAALEQAAVIAAASMREGYAT"
     gene            complement(10051..10497)
                     /locus_tag="VF752_07580"
     CDS             complement(10051..10497)
                     /locus_tag="VF752_07580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8855448.1"
                     /translation="MKVERSIELKASPEEVYEVVMDPSRLDDWVTIHAGLPEAPNGEL
                     RQGSELTQSLRIAGQRFNVHWKVVQADKPNRVVWEGRGPVRSKARVVYDFEPEGEGTR
                     FCYANEYHAPGGPLGRIADHALAGTSRREADRSLERLKKLVERNGR"
     gene            complement(10494..>11257)
                     /locus_tag="VF752_07585"
     CDS             complement(10494..>11257)
                     /locus_tag="VF752_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855449.1"
                     /translation="GLAEALDGVESAYYLVHSMEPAAGANGSFAGRDRRAAENFAEAA
                     AAAGVRRIVYLGGIVPSDGHLSPHLASRLEVERILLEAAPDSTALRASIVIGARSSSF
                     RILVRLVERLRVLPFPAWREHRTAPIDERDAIEYLARAPRTPAAAGRSLDIAGPDVLS
                     YAAMIERIAELMGVGRMPLPLGVNMTPVASPVVAAVTGQPVELVRPLMYSLERDLLPR
                     DDEARRIFDLRPHRFNRAVEHALGEWERDEELAAR"
BASE COUNT         1774 a         3801 c         3908 g         1774 t
ORIGIN      
        1 ttcgagatgc aggcggtcgc cccgaaggac agcatctggt tgcactcggc tgaggtcggc
       61 cggttcgcca gtaccacgat gcgcgagccg gggtgcgcct cgtgcaggtc gtgcacgtag
      121 cccggagagg gcagcgagcc gaagttcagc agcaccacct gcggctcgag ctcgtcgatc
      181 gcctcgtcga gctgctcgag cggcacgtcg ctcgccacga gctcgagatt cggatcctgg
      241 gtgatcagct gccgcagacc gacccgaacg agatcctcga acagcgcggt cacgacgctg
      301 acggcggatg aggggggtga agtcatctca ccccttatcc ccctatagcc ctccccctca
      361 aaccacccgg gggtgagcgc gcgcatcccc cgaagatccc tccaatttca ctcgccagag
      421 gggatgtgaa cgttcgttca gaagggcacc attcagacac ctcgacaggg gacgtcagaa
      481 aagacggggg ccacggaaga cctcgccgcc acggatggcg gaccaaagct ttctcagggc
      541 gaagtagggg tacgacgcca atcacacggg gaaccggcac agcgccggtt ctctcgtgtc
      601 aggggagaga cggatccaca gcggtcgccg ggaactgcgg gagttgtccg gcgaccgtag
      661 gatccggccc aatgacgccg gccatcgacg agcagtcgcg cgcgcgtgaa gccgccgccc
      721 gcgcggcggt cgaccgcatc caggctgggc agacgctcgg cttcggttcc ggtcgcgccg
      781 tctggaaggt gatcgagaca cttggcgagc gcgctccgga gcgtgcggtg gccgcctcca
      841 cccgcaccga ggagcggctt cgcgaggcgg gaatcgagcc gctatcgatc gacgacgccg
      901 gcacggtgga cctggtgatc gacggcgccg acgagctgga ccacagcctc aacctcatca
      961 agggaggcgg cggcgctcac ctgcgcgaga agctgctcgt ggaggcgggg cgcgatttcc
     1021 tcgtagtagc cgagacgccg aagctggtgg atcggctcgg taccaactgg ccggtacccg
     1081 tggagatcgt ccgcttcggc tgggttacca cgcgcgcccg cctgctcgag atctgtgcct
     1141 ccgcggagcg ccgcaccgac gaccgcggcc aggcttacgt gaccgacgag ggcaactgga
     1201 tcctcgactg ccggattccc gccgacggtg acctcgagga gctcgccgca gcgatcaagt
     1261 ccgagacggg cgtcatcgag cacgggctct tcctcggcat ggcgagcgcg gccatcctcg
     1321 gcacgccggg tggcgacgtc cagacgctga gcgcgtaggc ctccgtagcg aagacattcg
     1381 tcaactgcgg ttgacaacgg cgcggcgtca acctaaattg acgccatgac cgaagccacg
     1441 aaactcgccg agaaggtctc gagcaaagac cccgcggtcg gcctcaaggc ggttgcctcg
     1501 ctgcggacgc tgctcgagcg cctcgagcgg ctgcaggtcg agaacgcccg cgagcagggc
     1561 tggtcctggc agcagatcgc cgcagcactc gacgtgagca agcaggcagt gcacaagaag
     1621 cacggtggcg gcggcttgct cagccgcagg aggaagtaga tgttcacgcg attcgtgcag
     1681 agcgcccgga cggcggtggt cgaggctcag gcggctacgc gcgctcttgg ccatgacagc
     1741 gtcaccccga gccacatgct ggtcggcgtc gcgcgcgccg agccggagct cacgtcgcct
     1801 ctcaccgctg agcagcttca gtcggcgatt cgcgcgcccg gcatcgaccg tcgcgcgctc
     1861 gcgagcatcg ggatcgacta cgacgcggtc gagcgcgagg tggaggcgag cttcggcccc
     1921 ggcgcacttg cgagcgggcg cacgtgtgga caactcgcgt tcgcgccaga gtcgaagaag
     1981 gcgctcgagc tggcgctacg cgaagcacag gatcgcgagg acgagcacat cggtcccgag
     2041 cacctcctgc tcgcactcct cgacgaccac gacgtggccg aggtgatccg gcgctgcggg
     2101 gcggacccga aggagatgaa ggaccggctc taggccgccg cggccacgcc ggcgacgatt
     2161 cgctgcacgt cgtcggtgcc gatcacgtgc ttgttcatcc cgagctcctt cggcgagagc
     2221 ccgagcgcta gccccacgag ctgcggaaag tgcagcaccg gcaggccgag gtcgcgctcc
     2281 acgcgcttgg cggcgtccgg ctgctgcaga tcgaggttca ggtggcacag cgggcagggc
     2341 gtgaccatgc agtccgcgtc ggcatcgatc gcgtcggcga tgtgccggcc cgcctggcgc
     2401 agcgacgtct ccttgttcat cgtgatcagc gggaagccgc agcacttctg cttgcccgcg
     2461 tagtccaccg gctctccgcc gagagcctcg atcaccatct cgaggtagag gcgccgctcg
     2521 ggacgctcgt tcagcccgag gcggttgctc ggccgcacga tgtagcagcc gtagaacggc
     2581 gccaccctga gaccgtcgag cgggcggacg acgcgttcgc gaagcgagtc gagcccgatc
     2641 tcctcgacga gcaaccagag gaagttcttg ttccaccagt cgccgttgcg ctggtaggag
     2701 aggccctccg gcgcgaggtg ggagttcacg tggtcgcggt actcgtcgct cgcgtcgagt
     2761 cgctcctggc actccgtctg cgcgccctgg caggtcgagc agatgttcat catgccggcg
     2821 gctccctcga ccgactgggc cagtgcgaag gtgcgcgcgt tcagggagtc gacgagctcc
     2881 tggttgtgct cggcgatcac gccggcgccg cagcagttcg cgcggtcgag ctccaccagc
     2941 tcgatgtcga gcttcggggc gacggccgcc atggagccgt gcagctcggg ggtgaagccg
     3001 cgcgacacgc agccgggcca gtacgcgacc ttcacgattc tcgtcctcct tcggccatcg
     3061 tgccctcctc ctcgccgccg gactcgccga cgatgtagag gttgagctcg acgcgctcgt
     3121 ccttcgactc gatctccttg tagatgcgcc gaacctgctt ttggtccggc agcgtcgggt
     3181 gatggagcgc cttggccggg gcgaccttgc ccgacttgac gccgttcatc accgtgggca
     3241 atgactcggt gagctgcttg atgccgctcg ggtgcagggc cgccttgacg aagctgccgt
     3301 tgccatagga cctcgggagc agctgcgcct catgcaacgt gccgtacttc tggatgaggt
     3361 ccacgaacgc cttctcgtgg ccgtagccgt tgttgcggtc cttgatgccg tagtcgccgg
     3421 tggcgcggcg gcgcaggcgc atgatctggt ccatcggcgc gacgcccttc gggcagacct
     3481 ccacgcaggc gaagcagtgg gtgcagtcgt agatcccgtg cggatcctcg gcgaggtcgg
     3541 tgaggcgctc ctcggtggcg ccgtcgcgcg ggtcgccgac gaagcggtac gccttcgcga
     3601 gcgcggccgg accgatgaac tccgagtcca cctcgagcga gaggcaggcg cccacacatg
     3661 cgccgcagtg gatgcacgcc atggcctgcg tcacatcgat catcgattcg gccggcacga
     3721 tgtactcgcg ctccggcggg ggcccggccg gcagcagcca gggcacgatg cgctggacct
     3781 tcttccagtg gaccgcgtcc atgtccacga tcaggtcctt gagcacgggc atattgctca
     3841 tcggctccac cacgatcgcg ccgtcgtcgg tgaccgcgcg gtcggctgcc tcgccgatct
     3901 tcgtgttgca ggcgaggccg gtgcggccgt tgatgcgcac ggcgcaggag ccgcagatgg
     3961 ccgcctggca ggagcagcgg atggagatcg agccgtcgat gcggtcgcgc gcctgcagca
     4021 cgccgtcgag caccgagcgc tcgggcggga ggtcgacctg gaagtcctgc caataggcag
     4081 cctgcccgga ttccgggtcg aagcggcgga tcttcagcgt gtattcggcc attcgctccc
     4141 gttccctgac tctagtaacg gcgctcttcg ggctgccatt gggtgatcgt cacgtcggag
     4201 taggagatct ccggccggtc cccgttgagg ctcagatcga tgtgcttgag ccactcgtcg
     4261 tcgttgcgct ccggatagtc ggtccggaac tgcgcgccgc ggctctcccg acgctcgatc
     4321 gccgccgtga ccacacactc ggcgttgtcg agcatgaagc cgagctcgag agccgtgagc
     4381 acgtcctggt tgaagacggt ccccttgtcg tcggtcgcga cggtcttggc ctcgtccttc
     4441 agccggtcca cgatcccaag tgccgtccgg agaccgtcct cgtcgcggaa gacggcggcg
     4501 tacttgtcca tggtcgcgcc catctccgcc ttgatctccg aggtgcggcg gccggtgcgc
     4561 tcgcgtccga cgatctcctg gatgttccgc tcctcggtct cgacccacga gtggtcgggc
     4621 ttcggcatgg gcagcgtctg cccggtgcgc gccgcgtgct cgcccgagcg ccggccgaag
     4681 acgagcgtgt cgagcagcga gttcgcgccg aggcggttgc cgccgtggac ggacacgcag
     4741 gcgcactcgc cggcggcgta gaggcccggg atgttcgtct ggccgtcgat gtcggtgtcg
     4801 accccgccca tcgtgtagtg gttgccgggc ttcacgtgga tcggctcgcg ggtgatgtcg
     4861 acgccggcga agtcgcgccc caccatgacg atctcgcgga gcgcctcgtg gatgcgcttg
     4921 cgcgggacct tggtgatgtc gagcgccacg gtgccgtccg ggaagccgcg cccctcgttg
     4981 atctcggtct gctcggcgcg ggagacgacg tcgcgcgagg cgagctccat cttgttcggc
     5041 gcgtatttct ccatgaaccg ctcgccgagt gcgttgtaga ggtgcgcgcc ctcaccgcgg
     5101 gcgccctcgg tgatcagcag cccgttgccc tggagcgtgg tggggtggta ctgcaccatc
     5161 tccatgtcca tcagcttcgc gcccacccgg taggccatgg cgatgccgtc gccggtacag
     5221 atcagcgcgt tggtggtggg gttgtagatc tggccgttgc cgccggtcgc gaggatcacc
     5281 gacttcgcga cgaagagctc catcgagccg tcgcggatgt tgcgcgcgac tgcgcctgag
     5341 atgcggccgt tcccgtcgcg catcagcgag gtgacgaacc actcctcgta gcggtagacc
     5401 tcgttgtgct tcatcagctg ctcgtagagc acgtggagga tcgcctggcc ggtgatgtcc
     5461 gccacgtagt aggtgcgggc cgcggacgcg cctccgaagg cgcgagtgcc gaggcgcccc
     5521 tcctcgttgc ggtggaaggt cacgccgagg tgctcgaggt cgaggatctc gttgggcgcc
     5581 tcgcggcaca tgatctcgat cgcgtcctgg tcgcccaggt agtccgagcc cttgaccgtg
     5641 tcgaaggcgt gcgactccca gctgtcctcg ggattgaggg ccgcgttgat gccgccctgc
     5701 gccgcgttcg agtgcgagcg caccgggtgg accttggaca tgatccccac cgacgcgccc
     5761 ccgtgcgccg cggcgagcgc cgcgcgctga ccggccaggc cggccccgat gatgagcacg
     5821 tcgtgctgtg gcatctctgc ggtctttcct tctactcggc agctacccac cgggctcagc
     5881 ccggaggcgg cggcgattct atgcgaaccg ctcggccgct cgtcttcggc cccgccgagg
     5941 ggtcaggcgg ccgggatgct ggcccgaagc gccgcgcgca cacggtcgag cgtgaccacg
     6001 ccgcgcagca caccgtcgcg gtccaccgcc atcagggccc ccagccgctg cagtccctcg
     6061 gagccgagca gcgcgtcgag cgactcgtcg acgggcacct gtaggtcgtc gtcgcgcgac
     6121 ccgataaccg cctcccggac ctgccagccc tggcgcgtgt cgtggggcac ctggtccacg
     6181 cgatcgcggt cgagcacgcc cacgtagcgg ccgttctcat cgaccaccgg gaaccactgc
     6241 cagccgtagc ggaggaagta gtcgtcgtag gctcggtcga gcgggagcga tgccgggatc
     6301 gagaccggct cgtcgtccat cacgtccgcc accgagaccg acttgagacg cgtgctcagc
     6361 ccggtctgag cctcctccac gcgggcagcc tggtagatga agaacgcgat gaagatgtcc
     6421 cacacgccct cgatcaggta gccctggagg aacaggaaga cgccgagggc tccgagcagg
     6481 taggcgagcg cgacgccgag gcgagcggcg atgcgcgtgg cccgcaggcg gtcgccggtg
     6541 cgccaccagg cgatcgcccg caccatgcgg ccgccgtcga gcgggaagcc gggcaggatg
     6601 ttcagcacga acaggaacgc gttcacctcc gccagccagc cgaggagcag gacgcccggg
     6661 ctgatgtcgg cgcttccgct gaagcggacc gcgtccttga actcgtccgc gccgccgatg
     6721 gcgatcccga gcgccgtgca ggcgaccacc acgagcagcg tgacgatggg ccccgcaacg
     6781 gccaccttga actccgcgcc cgcggtgggt gcctcgcgtc gcatgcgtgc catgccgccg
     6841 aacagccaga gctcgatgcc ctcgatctcg attccgttgc ggacggccac gaacgcgtgg
     6901 cccagctcgt gcaacaggat cgagccgaag aacagcagcg cgctcgccac cgccagcgcg
     6961 aacgcggtgc tgtcgcttcc ggtgacgtcc ttgtagctgg ccgacagcga ccagatgagc
     7021 aggaacagga tgaagaacca gctcgggttg accccgatac gaattccgaa aacccgggcc
     7081 agctgcagcg agcgacctcc aaacaccagg gctgcatcgt cccatacggc cccgccgaac
     7141 ggcactcgtt accgcacgga cggcagcaga aagcgataat gcgcggcccc agagagagaa
     7201 aggagacccg tggcttatcg cgtcacgttc attcccggtg acggcacagg ccctgagatc
     7261 tcggaggccg tgcagcgggt gctcgaagcc acaggcgtcg acttcgagtg ggattttcag
     7321 gacgccggcg tggacgtcta cgaggccgag ggcacgccgc tgcccgatcg cgtgatcgag
     7381 tcgattcgcg agcgcgggct ggcgatcaag ggcccgatca ccactccggt cggctcagga
     7441 ttccgctcgg tcaacgttgc tctccgcaag gagctcgacc tctatgcgtg cctgcggccg
     7501 tgcaaggcct acgagggtgt gcgcacgcgc ttccccgaga ccgacatcgt gattgtccgt
     7561 gagaaccacg aggacctcta cgcgggcgtc gagtacgaga tcggcgaccc gaacacggag
     7621 aagctgcgcc agttcatcaa ggacaccgag ggcaccgagc tgcgtgagga catcggtgtc
     7681 tcgatcaagc cgatctcggt ctacggcaca cagcggatcg tgcgcgaggc gttcgagtac
     7741 gcgaaggcca atgggcgctc gaaggtcacc ctcgcgcaca aggccaacat catgaagcac
     7801 accgacggcc tcttcctgcg tgtcggacgc gaggtggcgg agcgggagta tccggacgtc
     7861 gagttcgagg accggatcat cgacaacctc tgcaaccagc tcgtgtcgcg gcctgaggag
     7921 tacgacgtga tcgtgctgcc gaacctctac ggcgacatcg tttcggacct gggcgccggc
     7981 atgatcggcg gcctcggtct cgcgcccggc gcgaacgtgg gcgacgacta cgcggtgttc
     8041 gaggccgtgc acggctcggc gccgaagtac aagggccaga acaaggtcaa cccgatggcg
     8101 ctgatgcttt ccggcgtgat gctgctccgc cacatcgacg agcgcgaggc cgcggagcgc
     8161 atggaggctg cgatcgcgga agtgatccgc gagggtaagt cggtgaccta cgacatgaag
     8221 ccgacgcgcg acgacccgac cgccgtcggc acgtcggacg tggccacggc aatcatcgaa
     8281 aaaatggaga aggtcgctgt ttagctactc ggggcgcgac tcacgccgcg gtgtatccgc
     8341 ggccttgctt cttgactgca ccctcggatt cgaggttggc catcacccgg tacatgtagt
     8401 tgggcgggct caccccgagt tcgcgggcga tgtcgctgac cctgatgccg gggttctgcc
     8461 ggacgatggc gagcgcctgc tgggcgcggt tgccggttcc acgcggacgg ccgcggcggc
     8521 gcctgccgtt gctgctcgtg ccgctcgtgc gcgggcggcc gggaccgcgg cgaggcgcgt
     8581 cgacaccctc gagtgcggcc ttcgctcgct cgagcttcag gtactcgtcg tggagcgggc
     8641 gaagctcctt gagccgggtc tcgatctcgg cccgtttctc ggccagaaag tcagccatga
     8701 caccacctcg tgtggttagt gaaaaaggtt aaagccttca ggaaaaaggt agggataaat
     8761 gcgtagaaag attactgtag ttggtgcagg taatgtaggt gcaaccactg cacagcggct
     8821 tgcggaacgt gattacgcgg acgttgtggt cgtcgacatc gtcgagggca tgccgcaggg
     8881 caaggcgctc gacctgaacc agtccggccc ggtggtcggc tacgagccga acgtggtcgg
     8941 gacgaacggc tacgacgaga cggccggctc ggacatcgtc gtgatcacct ccggcttccc
     9001 gcgccagccg ggcatgagcc gcgacgacct gctctcgaag aacaaggaga tcgtcggtgg
     9061 cgtggccgcg caggtggccg agcgctcgcc ggacgcgatc atcgtcgtcg tcaccaaccc
     9121 gctcgacgcg atgtgtcacg tggccctcga cgtaagcggc ttcccgcgcc agcgcgtgat
     9181 cggcatggcg ggcatactcg actccgcgcg cttccgcacc ttcctcgcct gggagctcgg
     9241 cgtgtccgcg cgcgacgtga cgggcttcgt gctcggtggt cacggcgata cgatggtgcc
     9301 ggtcgtgagc tacacgaacg tcgccggcgt gcccgtgcgg cagaagatct ccgatggccg
     9361 gctcgaggag atcgtgcagc gcacgcgtga tggcggcgcc gaggtggtca agctgctgca
     9421 gaagggctcg gcgttctacg cgccgtcggc ggccgtcgcc gagatggtcg attcgatcgt
     9481 cttcgaccag aagcgggtgc tgccgtgcgc cgccctctgc cagggcgagt tcggcatcga
     9541 cggcctgttc gtcggcgtgc cggtgcggct cggggccgag ggcgtggagg agatcgtcga
     9601 gatcgagctc gacgacgccg agcgcggcga gctcgagaag tcggcggacg ccgtgcgcga
     9661 gctggtcgac gcgatgaagg ctctctaggc ggcggccgcg agggctacgt cgcgtagccc
     9721 tcgcgcatgc tcgcggccgc gatcacggcg gcctgctcga gcgcggcagc ccagctgcca
     9781 catgcgcgcg tgagcgtggt gattcgcggc aggcccgcgc cggggagccg gtcgcgaatc
     9841 gccacgtagc ggtcctcgct cagctcgttc agcgcgcacc cgtcgatgtc gcgggcgcgc
     9901 aggacggcct tggcggcagc ctcgcagtct cgccacggcg gcctggcgaa cggcgaccag
     9961 ccgccggcgg cgcgagtccc aatcgagtcg ctcacatcgc cctcctaccc tgcggaagcc
    10021 gacggccaaa cgggttgcgg gccggcgagg tcagcgcccg ttccgctcca ccagcttctt
    10081 gagccgttcg agggagcggt cggcttcgcg cctcgaggtg cccgccagcg cgtggtcggc
    10141 gatcctgccg agcgggccgc ccggcgcgtg gtactcgttg gcgtagcaaa agcgggtgcc
    10201 ctccccttcg ggctcgaagt catacacgac acgggctttc gacctcacag ggccccggcc
    10261 ctcccagacg acgcgattcg gcttgtcggc ctgcacgacc ttccagtgca cgttgaaccg
    10321 ctgcccggcg atgcgcagcg actgcgtcag ctccgatccc tgcctgagct cgccgttcgg
    10381 cgcctccggc agtcccgcgt ggatggtgac ccagtcgtcg aggcggctgg gatccatcac
    10441 cacctcgtag acctcctccg gcgaggcctt cagctcgatg ctgcgctcca ccttcatcgt
    10501 gccgccagct cctcgtcgcg ctcccactca ccgagcgcgt gctcgacagc gcggttgaag
    10561 cggtgggggc gcagatcgaa gatccgccgt gcctcgtcgt cccggggcag gaggtcacgc
    10621 tcaagggagt acatgagggg tcggacgagc tcgaccggct ggccggtcac ggcggccacg
    10681 acagggctag ccaccggggt catgttcacg cccagcggga gcggcatccg cccgacgccc
    10741 atgagctccg cgattcgctc gatcatcgcc gcgtaggaga gcacgtccgg cccggctatg
    10801 tcgagcgagc gccccgccgc agcaggcgtc cgaggcgccc gcgcgaggta ctcgatcgcg
    10861 tcgcgctcgt cgatcggcgc ggtgcgatgc tcgcgccaag ccgggaaggg cagcacgcgc
    10921 agccgctcca cgagccgcac caggatccgg aacgacgacg agcgcgcgcc gatcacgatc
    10981 gaggcgcgca gcgccgtgga atccggcgcc gcctcgagca ggatccgctc tacctccagt
    11041 cgcgaggcga ggtggggcga gaggtggccg tccgacggca cgatgccgcc gaggtagacg
    11101 atccgccgca cgcccgcagc cgcggcagcc tcggcgaagt tctcggccgc gcggcggtca
    11161 cgacccgcga agctcccgtt cgcgccggcg gccggctcca tcgagtgaac gaggtagtac
    11221 gcgctctcca cgccgtcgag cgcctcggcc aggccct
//
LOCUS       DASVXS010000139        10436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_532937, whole genome shotgun sequence.
ACCESSION   DASVXS010000139 DASVXS010000000
VERSION     DASVXS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532937"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1961
                     /locus_tag="VF752_07590"
     CDS             15..1961
                     /locus_tag="VF752_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8855450.1"
                     /translation="MTTVAARAEAGAGPDGERLRHFLDGEHRWIRERVREVLSRPDFA
                     PVSGVTREEHRERVLQWMRALAEEGGTKLGFPEEYGGMGTVGGSIAAFETLGHADLSL
                     LVKCGVQFGLFGGAVLHLGNAQHHERYLPGVMSAELPGCFAMTETGHGSNVQALGTTA
                     TYEPETGEFVVNTPHEESRKDYIGNAALHGRMAAVFAQLEVGGESHGVHCLLVPLRDE
                     DGNVLPGIRIEDCGEKLGLEGVDNGRIWFDHVRVPREALLDRYASVDEQGRYSSPIEN
                     PNRRFFTTIGTLIQGRISVGGAAISATKSALTIAIRYALERRQFGPPDSDEEVLLLDY
                     RVHQRRLLPQLAKTYALHFAQEQLVAQLHQAFTEPDYPERERRMLEALAAGVKATATW
                     HATETIQTCREACGGAGYLAENRFAAMKADTDVFTTFEGDNTVLLQLVAKGLLTDYKE
                     RFADLSQLEMVRFVGAQVWDTVVERSLARQLAGALADAVPSRDEDRPIEDPAYLMDLF
                     RWREEHILGGVARRLKRGIDSGADSFTVFNECQDHVLAAARAHVDRLILDAFREGIDR
                     CDDPALTELLESLFHLYALSNVEADRGWFFEHGRMNGPRSKAITRRVNDLCGDLRAHA
                     ELLVDAFGIPDAVLRAPIGVKHPE"
     gene            complement(1962..2043)
                     /locus_tag="VF752_07595"
     tRNA            complement(1962..2043)
                     /locus_tag="VF752_07595"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2007..2009),aa:Tyr,seq:gta)
     gene            2105..2299
                     /locus_tag="VF752_07600"
     CDS             2105..2299
                     /locus_tag="VF752_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855451.1"
                     /translation="MTFLPLRLAAAAATAPFVLVRVVQRLDEISRTLELLTATDGPIR
                     RPGGVGDRLDRLTAEGGPLD"
     gene            2318..2677
                     /locus_tag="VF752_07605"
     CDS             2318..2677
                     /locus_tag="VF752_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855452.1"
                     /translation="MTIALENLSELQEALLKLAALEASLDRIAAVAASLERLAEVSSS
                     LPDLAQAASSLPELPAHVRGIERQVQRVESQLGRLEPSLNTLGASVVEVQDAVERLSE
                     SLAPIGRLAGRVPGSRA"
     gene            2784..3803
                     /locus_tag="VF752_07610"
     CDS             2784..3803
                     /locus_tag="VF752_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX8855453.1"
                     /translation="MPNEFDKRRLAKRMFEVLMILLVFLLIVLLAPGLGDVRDKLVDA
                     DPGWLVLGIVLEALSCLSYVLMFRPVFCRHMSRRTAREIAWSELGVGSLVPASGAGGL
                     ALGAWVLSRGGMPADKIARRSVAFFLIKSSVNFVAVALIGFVMAIGLVGPHKSPWLTA
                     GPALGATIAIGLILLVPRLGPGKERGPDASGFGRALSATRRALIGGVDEAIGIVRSGN
                     AAVIAGAIGYWLFDNAVLWATFHAFGYSPPISIILMGYLIGQLGGLLPLPGGLGGIDG
                     GLIGTLVVYGLPAAGTTAAVLAYRVILFWLPLIVGAIAFVSLRRGLNDPERPDLCNVP
                     EPAPA"
     gene            complement(3800..4936)
                     /locus_tag="VF752_07615"
     CDS             complement(3800..4936)
                     /locus_tag="VF752_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8855454.1"
                     /translation="MSAKDDVVLPVEQPDAPFAEIEQLSGLVAEGRERGYLTFEEIAA
                     TLEEVEVSKEQIQELHAHLLEHGVDVVSHDGKPMSVDAAVVSENKDAGSAKKVEIDLT
                     VEPSLDSLRLYLRSIGRVGLLTAYEEVELAKRIERGDMGAKQHMIEANLRLVVSIAKG
                     YLGRGLSFLDLIQEGSLGLIRAVEKFDYRRGYKFSTYATWWIRQAVTRAIADKARTIR
                     IPVHMVEKLNKVVHVERQLVQDLGREPTPEEIARELECSPREVRDILRMAQLPVSLEK
                     PIGEEEDSELGDFVEDQTAESPFELAVENLRRENVRKALDALPAREREVIEMRFGLKG
                     HQARTLEEVGRAFGVTRERIRQIENNTLKKLESLPEAQRLRDAS"
     gene            5163..6149
                     /gene="mptA"
                     /locus_tag="VF752_07620"
     CDS             5163..6149
                     /gene="mptA"
                     /locus_tag="VF752_07620"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HEX8855455.1"
                     /translation="MTLLSRTSDDVQGRRPTAHISLSRVGVTGVEKVIRIRSNGGEQL
                     FYAELECFVDLGPKQKGAHMSRFEETVNDAIAEVVLGEAFKAETLAQHIAQRVRERQD
                     ALRAEVTVAARYPEHKPAPVSGQMTQEIYTLYGSAVVSEQGTRRLVGVQAQGMTACPC
                     AQELVISRARERLGSDGFSEEEIERVLEAVPVATHNQRGLGTLWVGCPEACSAEIEAS
                     TLLHIVEESMSSEIYELMKRPDEVEVVEKAHRRPRFVEDCVREAIRMAVEEFRPLGDD
                     IFIMSRQENLETIHQHNVVAERFGVIGELARELEAGEHVRHHVSMREWLDGA"
     gene            complement(6154..7071)
                     /locus_tag="VF752_07625"
     CDS             complement(6154..7071)
                     /locus_tag="VF752_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX8855456.1"
                     /translation="MCRRLLPRAFVLFAAAIAVAGCSSAGSSASGGGPVVVATTTQLA
                     DIARNVAGPEAKVEQILRPNTDPHEYEPRPGDAIAVSKAKVVLRSGGEVDSWLEGVLN
                     QASSGARVVNVGAGVKLRGDDPHWWQDPQNAELATRAIASALAAADSKHAAAYRQRAE
                     AYARKLRTLDGAIARCMGRLPASERRLVTNHDALEYFAHRYRVTVVGVVIPSLSTAAG
                     ASAGETAKLVKTIRREYVREIFPESSVPKGVEAAVAHEAGAKLGPALYADSLGPKGSP
                     GATYLGSLRFNATAMADGFSGRPGSCRLH"
     gene            complement(7083..7679)
                     /gene="pgsA"
                     /locus_tag="VF752_07630"
     CDS             complement(7083..7679)
                     /gene="pgsA"
                     /locus_tag="VF752_07630"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX8855457.1"
                     /translation="MFPLNVPNVLTVIRILLVPVLVAALLVETPRGATIAAIVFALAA
                     FTDGLDGYIARSRQSITTFGKVMDPIADKLLIASALISLVSLNRVDAWVAMVIIAREF
                     AVSGLRIAAGAQGAVIPASFLGKVKTVSQVAAVLALIAAGDPNAAWVQALVYVSVVIT
                     VVSGIDYFLNVRKRIEEVRRDVTAAARERLTANENHSQ"
     gene            complement(7691..7987)
                     /locus_tag="VF752_07635"
     CDS             complement(7691..7987)
                     /locus_tag="VF752_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855458.1"
                     /translation="MSYFRAVRIYSAFESLLFTSLLVVWIGGFDESLKQALGWAHGFG
                     WIFLCLLVANGCRKRVFPWWLLAFTVSPFGPLGSSLGIELLARERRGMAERPAG"
     gene            complement(7998..8384)
                     /locus_tag="VF752_07640"
     CDS             complement(7998..8384)
                     /locus_tag="VF752_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8855459.1"
                     /translation="MQISNLSDLESFTTLDGSRIRELAGPAWTAARNQSLAEATVPAG
                     GETQEHYHAVTEELYYFTAGAGRMRLGTEERAVRAGDCVVIPPGTPHKLWASEDEPLV
                     LLCCCSPAYRDSDTVMTEEPPRQRRH"
     gene            8525..9928
                     /locus_tag="VF752_07645"
     CDS             8525..9928
                     /locus_tag="VF752_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8855460.1"
                     /translation="MNPPTATSHPATGVDELRARIAGDYVTPADPNWDEARLAWNLAV
                     DQRPAAVALPETAEDVAEVVRYARDNGLKMAPQSTGHNAHPLELAQTVLVKTERLRSV
                     EIDPEARIAIIGGGAIWQDVTHPAAEHGLAALAGSSPDVGVLGYSLGGGLSWLARSKG
                     LAANMIEAIEIVTADGEIVRADADNEPDLYWAVRGGGGNFGVVTAIEMDLIPLTEVHA
                     GVLFFPIERAHEVLHAWREWVKNVPDEVTSVGRLLRFPPLEEIPEPVRGGKFAVIEAT
                     MLVDEAEAADLLAPLRALGPAMDTFATIPVTELQHLHMDPPHPVPGKGDGLLLRDLPA
                     KALDSLLEVTGPGVESPLLSVEIRHIGGAAGRPDPNGGALDSIDAPFVMFAVGMTPVP
                     EMVEAVEAHVNIAKRALGAWDRGLMYLNFAEHKIDPSRLWSPAAYLRLRKIRAAYDPD
                     ELFRANHPVPPFPVAAE"
     gene            10077..>10436
                     /locus_tag="VF752_07650"
     CDS             10077..>10436
                     /locus_tag="VF752_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855461.1"
                     /translation="MRKYLSVTLLLAAASLAAPAAASASATVLGTTSSSLGSPALAGD
                     RVVATVFRSANLEQLYSFAPGTPAKRLLTNRTRFEGDFAVLGYGASAQRVAAVRVGEA
                     SPGNAQFFSGPPLGPLSQ"
BASE COUNT         1543 a         3687 c         3591 g         1615 t
ORIGIN      
        1 catgatccgc cgccatgacc accgttgctg cgcgggccga ggcgggcgcg gggccggacg
       61 gcgagcggct gcgccacttc ctcgacgggg agcaccgctg gatccgggag cgcgtgcgcg
      121 aggtgctgag ccggccggac ttcgcgccgg tgtctggcgt cacccgagag gaacatcggg
      181 agagggtgct gcagtggatg cgggcgctcg ccgaggaggg cggcacgaag ctcggcttcc
      241 ctgaggagta cggcgggatg ggcaccgtcg gcggctcgat cgcggcgttc gagacgctcg
      301 gccacgccga cctctcgctg ctcgtgaagt gcggcgtgca gttcgggctg ttcggcggtg
      361 cggtgctcca cctcggcaac gctcaacacc acgagcgcta cctgcccggc gtcatgagcg
      421 cggagctgcc cggctgcttc gccatgaccg agacgggaca cggctcgaat gttcaggcgc
      481 tcggcacgac ggcgacatac gagccggaga ccggcgagtt cgtggtgaac actccccacg
      541 aggagtctcg caaggactac atcggcaacg cggcgcttca cggacgcatg gccgccgtct
      601 tcgctcagct ggaagtgggt ggcgagtcgc acggcgtgca ctgcctgctc gtgccgcttc
      661 gcgacgagga cggcaacgtg ctgcccggca tccgcatcga ggactgtggc gagaagctcg
      721 gcctcgaggg cgtcgacaac ggtcggatct ggttcgacca cgtgcgcgtg ccgcgcgagg
      781 cgctgctcga ccggtacgcg agcgtggacg agcagggccg ctactcgagc ccgatcgaga
      841 accccaatcg gcgcttcttc acgacgatcg gcacgctgat ccagggccgc atcagcgtgg
      901 gcggtgcggc gatcagcgcc acgaagtcgg cgctgacgat tgcgatccgc tacgcgctcg
      961 agcggcgaca gttcgggcca cccgacagcg acgaggaggt gctcctactc gactaccgag
     1021 tgcaccagcg gcgactgctg ccgcagttgg cgaagaccta cgcgctgcac ttcgcgcagg
     1081 agcagctcgt cgcgcagctc catcaagcgt tcaccgagcc cgactatccc gagcgtgagc
     1141 gccggatgct cgaggcgctt gcagcgggcg tgaaggcaac ggcgacctgg cacgccacgg
     1201 agacgatcca gacctgccgc gaggcctgcg ggggcgccgg ctacctggcg gagaaccgct
     1261 tcgccgcgat gaaggccgac accgacgtgt tcaccacctt cgagggcgac aacacggtgc
     1321 tgctgcagct cgtcgcgaag gggctgctga ccgactacaa ggagcgcttc gccgacctca
     1381 gccagctcga gatggtgcgg ttcgtgggcg cccaggtgtg ggataccgtg gtcgagcgca
     1441 gcctggcacg ccagctagcg ggcgcgctgg ccgacgcggt tccgagccgc gacgaggacc
     1501 ggccgatcga ggatccggcc tacctcatgg acctcttccg ctggcgggag gagcacatcc
     1561 tcggcggtgt ggctcggagg ctgaagcgag gcatcgactc gggcgccgac tcgttcaccg
     1621 tcttcaacga gtgccaggac cacgtgctcg ctgcggcacg ggcgcacgtt gaccggctca
     1681 tcctcgacgc cttccgtgag gggatcgacc gctgcgacga cccggcgctc accgagctgc
     1741 tcgagtcgct gttccacctc tatgcgctct cgaacgttga ggccgaccgc ggctggttct
     1801 tcgagcacgg ccgcatgaac ggcccgcgct cgaaggcgat cactcgacga gtgaacgacc
     1861 tctgcggaga cctccgtgct cacgccgagc ttctggtgga cgcgttcggg atcccggacg
     1921 cggttctgcg ggcgccgatc ggcgtcaagc accccgagta gtggggggag caggattcga
     1981 acctgcgtag gcatagccaa cgggtttaca gcccgtctcc tttaaccact cggacatccc
     2041 cccgagcggg gtccgatgat agagccggcg cccgcaggcg tgccgccttg tatcaatccc
     2101 gtccgtgacc ttcctccctc tgcgcctggc agccgccgct gccaccgcgc cgttcgtgct
     2161 cgtgcgcgtc gtccagcggc tcgacgagat ctcgcgcacg ctcgagctgc tcaccgccac
     2221 ggacggcccg atccgccgcc caggcggcgt tggcgatcgg ctcgaccggc tcaccgccga
     2281 gggcggcccg ctcgattagc tggacgggat cgcggaggtg accatcgcgc tcgagaacct
     2341 ctccgagctc caggaggcgc tgctgaagct cgccgcgctc gaggcgagcc tcgaccgcat
     2401 cgccgccgtg gccgcgagcc tcgagcgcct cgccgaggtg tcgtcgagcc tgcccgacct
     2461 cgcccaggcg gcgagctcac tgccggagct gcccgcgcac gtacgcggga tcgagcggca
     2521 ggttcagcgg gtggagagcc agctcgggcg actagagccg agcctcaaca cgctcggcgc
     2581 gtcggtcgtc gaggtacagg acgcggtgga gcggctcagc gagtcgctcg cgccgatcgg
     2641 gcggctcgcc ggccgagtgc cgggttcgcg cgcttgattg ctagtgcaag aatcggagca
     2701 tcgcttccga tccgcagcag acccaagcgc agcgacgacc agccgtaacg gtacgcccgc
     2761 tcgagggccc cgcgccggtg ccgatgccga acgagttcga caagcggcgt ctggccaaga
     2821 ggatgttcga ggtgctgatg atccttctgg tcttcttgct gatcgtcctg ctcgcgccgg
     2881 gcctcggcga cgtacgcgac aagctcgtcg acgccgaccc cggctggctg gtgctgggca
     2941 tcgttctcga ggcgctttcg tgcctctcgt acgtactcat gttccggccc gtgttctgtc
     3001 gccacatgtc gaggcgcacg gcgcgggaga tcgcctggtc ggaactcggc gtcggctcac
     3061 tcgtgccggc gagcggcgcg ggcgggctcg cgctcggcgc ctgggtgctg agccgcggtg
     3121 gcatgcccgc cgacaagatc gcccgccgct ccgtcgcgtt cttcttgatc aagagctcgg
     3181 tgaacttcgt cgcggtcgcg ctgatcggct ttgtgatggc tatcgggctc gtcggcccgc
     3241 acaagtcgcc ctggctcacc gccggtcccg ccctgggagc cacgatcgcg atcggcctga
     3301 ttctgttggt gcctaggctg ggtccaggaa aggagcgcgg gccggacgcg tcgggcttcg
     3361 ggagggcatt gtccgcgact cgccgagcgc tgatcggagg cgtcgacgag gcaatcggga
     3421 tcgtgcgctc cggcaacgcg gctgtgatcg caggggcgat cggctactgg cttttcgaca
     3481 acgccgtgct ctgggcgacc ttccacgcgt tcggctactc gccgccgatc tcgatcatcc
     3541 tcatgggcta cctgatcggc cagctcggcg ggctgctgcc gctgcccggt ggactcggcg
     3601 gcatcgacgg cgggctgatc ggcacccttg tcgtctacgg cctgcccgcc gcgggaacga
     3661 ccgcggccgt cctcgcctac cgcgtgatcc tcttctggct gccgctgatc gttggcgcga
     3721 tcgcgttcgt ctcgctgcgc cggggcctga acgaccccga gcgtcccgac ctctgcaacg
     3781 tgccggagcc ggcgcccgcc taggacgcgt cccgcagccg ctgggcctcg ggcaggctct
     3841 cgagcttctt cagcgtgttg ttctcgatct ggcggatgcg ctcgcgcgtg acgccgaacg
     3901 cgcgtcccac ctcttcgagc gtgcgcgcct ggtggccctt gaggccgaag cgcatctcga
     3961 tcacctcccg ctcgcgcgcg ggcagtgcgt cgagcgcctt gcgcacgttc tcgcgccgga
     4021 ggttctccac cgccagctcg aacggcgact cggcggtctg gtcctccacg aagtcgccga
     4081 gctcggagtc ctcctcctcg ccgatcggct tctcgagcga caccggcagc tgcgccatcc
     4141 gcaggatgtc gcgcacctcg cgcggggagc actcgagctc ccgggcgatc tcctccggcg
     4201 ttggctcgcg gcccaggtcc tgcacgagct gacgctccac gtgcacgacc ttgttgagct
     4261 tctcgaccat gtggaccggg atgcggatcg tgcgcgcctt gtcggcgatc gcgcgcgtga
     4321 ccgcctggcg gatccaccag gtcgcgtagg tcgagaactt gtagccgcgc cggtagtcga
     4381 acttctccac cgcgcggatc agcccgagcg aaccctcctg gatcaggtcg aggaatgaga
     4441 gcccgcgccc gaggtagccc ttcgcgatcg acacgacgag gcggaggttc gcctcgatca
     4501 tgtgctgctt cgcccccatg tccccgcgct cgatgcgctt ggcgagctcg acctcctcgt
     4561 aggcggtgag caggccgacg cgaccgatgg agcgcaggta aaggcgcagc gagtcgaggc
     4621 tcggttcgac ggtcaggtcg atctcgacct tcttcgcaga acccgcgtcc ttgttctcgc
     4681 tgaccacggc ggcgtcgacg gacatcggct tgccgtcgtg ggacacaacg tcaacgccgt
     4741 gctcgagcag gtgagcgtgc agctcctgga tctgctcctt ggagacctcg acctcctcga
     4801 gagtcgccgc gatctcctcg aacgtcaggt agccgcgctc gcgcccctcg gccaccagcc
     4861 cgctcagctg ctcgatctcg gcgaacggcg cgtcgggctg ctccaccggg agcacaacgt
     4921 catccttagc gctcaacccc tcgctcctat ctcttccctc ggtcggactt acagctactg
     4981 cgagccgtgc cctctccact gacacgaaag acgtatttcg gatctgagtt cgcgggtgcg
     5041 ccgcgatggg ctgagccaca tttccggggc tggccagccc cccggcgtcg agcctgcggc
     5101 cagtatacgt gcggatcgtt gttttgccac cgagcggctc tcgctatcgt caactggtcg
     5161 caatgaccct cctgagccgc acttccgacg acgttcaggg ccgccgaccg acggcccaca
     5221 tcagcctctc gcgcgtgggc gtgaccggcg tcgagaaggt catccgtatc cgctcgaacg
     5281 gcggcgagca gctcttctac gccgagctcg aatgcttcgt ggacctcggc ccgaagcaga
     5341 agggcgcgca catgtcgcgc ttcgaggaga cagtgaacga cgcgatcgcc gaggtagtgc
     5401 tcggcgaggc cttcaaggct gagacgctcg cgcagcacat cgcccagcgc gtgcgcgagc
     5461 gccaggacgc gctgcgggcc gaggtgacgg tggcggcgcg ctacccggag cacaagccgg
     5521 cgccggtgtc gggccagatg acgcaggaga tctacacgct ctacggctcg gccgtggtct
     5581 ccgagcaggg cacccgccgg ctcgtgggcg tgcaggccca ggggatgacc gcgtgtccgt
     5641 gcgcgcagga gcttgtcatc tctcgcgcgc gcgagcgcct cggcagcgac gggttcagcg
     5701 aggaggagat cgagcgcgtg ctcgaggccg tgcccgtggc gacccacaac cagcgcgggc
     5761 tggggacgct gtgggtcggc tgcccggagg cctgcagcgc ggagatcgag gcctccaccc
     5821 tcctgcacat cgtcgaggag tcgatgagct ccgagatcta cgagctgatg aagcggcccg
     5881 acgaggtcga ggtcgtcgag aaggcgcacc ggcgcccgcg cttcgtggag gactgcgtgc
     5941 gcgaggcgat ccgaatggcc gttgaggagt tccggccgct aggcgacgac atcttcatca
     6001 tgtcgcgcca ggagaacctg gagacgatcc accagcacaa cgtcgtggcg gagcgcttcg
     6061 gcgtgatcgg cgagctcgcg cgagagctgg aggccggcga gcacgtgcgc catcacgtgt
     6121 cgatgcgcga gtggctcgac ggcgcctgag cgcctagtgc aggcggcacg agccggggcg
     6181 gccgctgaag ccgtccgcca tcgccgtcgc attgaagcgc agcgagccca ggtaggtggc
     6241 gccgggcgat ccctttggcc cgagcgagtc ggcgtacagc gccggcccga gcttggcgcc
     6301 ggcctcgtgc gcgacggcgg cctccacgcc cttcggcacc gagctctccg ggaagatctc
     6361 gcgcacgtac tcgcggcgga tcgtcttcac gagctttgcg gtctcgccgg ccgaggcgcc
     6421 cgcggcggtg gagagcgacg ggatcaccac gccgaccacc gtcacgcgat agcggtgcgc
     6481 gaagtattcg agcgcgtcgt ggttcgtcac caggcggcgc tcgctggccg gcagccggcc
     6541 catgcatcgc gcgatcgcac cgtccagcgt cctcagcttg cgggcgtacg cctccgcccg
     6601 ctgccggtag gcggcggcgt gcttcgagtc ggcggcggct agcgcggacg cgatcgcccg
     6661 cgtggccagc tcggcgttct gcgggtcctg ccaccagtgc gggtcgtcac cacgcagctt
     6721 cacgcccgcc cccacgttca ctacccgcgc gcccgagctt gcctggttca gcacaccctc
     6781 gagccacgag tcgacctcac cgccggagcg cagcaccacc ttcgccttcg acacggcgat
     6841 cgcgtcgccc ggccgcggtt cgtactcgtg cgggtcggtg tttggccgga gaatctgctc
     6901 gaccttcgct tccgggccgg cgacgttgcg ggcgatgtcc gccagctgcg tggttgttgc
     6961 cacgaccacc ggccccccgc ctgaggcgga gctacccgcg ctcgagcagc cggcgacggc
     7021 gattgcggcg gcgaagagca cgaacgcccg cgggagcagc ctgcggcaca tggccgctca
     7081 gcttactgcg aatgattctc atttgcagtt aggcgctccc gagctgccgc cgtcacgtcg
     7141 cggcgcacct cctcgatgcg cttgcggacg ttgaggaagt agtcgatgcc ggacaccacg
     7201 gtgatcacca ccgacacgta cacgagcgcc tggacccagg cggcattcgg gtcgccggcc
     7261 gcgatgaggg ccaacactgc ggccacctgc gagacggtct tgaccttgcc gaggaagctc
     7321 gccgggatga ccgccccctg cgcgccggcc gcgatgcgaa ggccactcac cgcgaactcg
     7381 cgcgcgatga tcaccatcgc gacccaggcg tccacccggt tgaggctcac gagcgagatg
     7441 agcgcggagg cgatcagcag cttgtccgcg atcgggtcca tcaccttccc gaaggtggtg
     7501 atgctctgcc gcgagcgcgc gatgtagcca tcgagcccgt cggtgaaggc ggcgagggcg
     7561 aacacgatcg cggcgatcgt ggcgccgcgc ggagtctcca ccagcagcgc cgcaaccagc
     7621 accggaacca gcaggatccg gatgactgtg agtacgttcg gcacgttgag cgggaacacg
     7681 cccgctcagg ctaccccgcc gggcgctctg ccatgccgcg tcgctcgcgc gcgagtagct
     7741 ctattccgag cgagctgccg agcggcccga acggcgagac cgtgaacgcg agcagccacc
     7801 acgggaacac gcgcttgcgg cagccgttgg ccacgagcag gcagaggaag atccagccga
     7861 agccgtgcgc ccacccgagc gcctgcttga ggctctcgtc gaagcccccg atccagacca
     7921 cgagcagcga cgtgaacagc agcgactcga acgccgagta gatgcgcacc gcgcggaagt
     7981 agctcacgcg cccgagacta gtgccggcgc tgccgcggtg gctcttcggt catcaccgtg
     8041 tcgctgtccc tgtacgcggg cgaacagcag cacaggagca cgagcggctc gtcctcggac
     8101 gcccagagct tgtgcggagt gccgggtggg atgaccacgc agtcgcccgc tcgcacggcg
     8161 cgctcttccg taccgagccg catgcggccg gccccggcgg tgaagtagta gagctcctcg
     8221 gtcaccgcgt ggtagtgctc ctgcgtctcg ccacccgccg gcaccgtggc ctcggccagg
     8281 ctctggttgc gagccgcggt ccaggcaggc ccggcgagct cccggatccg tgagccgtcg
     8341 agcgtggtga aggactcgag gtcactcagg ttcgagatct gcacgtgcgg atcttcgcac
     8401 gcgacgcatg ccggatatcc gcaccagcgc ccgcatgctc accccctggg agcgcgcgtc
     8461 cgcgcaggcg aatctcccag tgcgcctact tcgactcgca ctgacccagg aggcactccc
     8521 gaccatgaat ccgccaaccg ccaccagcca cccggccacc ggcgtcgacg agctgcgcgc
     8581 tcgcatcgcc ggcgactacg taaccccagc ggacccgaac tgggatgagg ctcgcctcgc
     8641 gtggaacctc gccgtcgacc agcgccccgc cgcggtggcg ctccccgaga cggccgagga
     8701 cgtggcggaa gtcgtgcgct acgcccgcga caacggcctc aagatggcgc cgcagagcac
     8761 ggggcacaac gcccatccgc tcgagctggc gcagaccgtg ctggtgaaga cggagcgcct
     8821 gcgctcggtc gagatcgacc ccgaggcgcg catcgccatc atcggcggcg gcgcgatctg
     8881 gcaggacgtg acccaccccg cggcggagca cggcctggcc gcgctcgccg gctcctcgcc
     8941 cgacgtcggc gtgctcggct actcgctcgg cggcggtctt tcttggctcg cccgcagcaa
     9001 gggccttgcg gccaacatga tcgaggcgat cgagatcgtg accgccgacg gtgagatcgt
     9061 gcgcgcggac gccgacaacg agcccgacct ctactgggcc gtgcgcggcg gcggcgggaa
     9121 cttcggcgtg gtcaccgcga tcgagatgga cctgatcccg ctcacagagg tccacgccgg
     9181 cgtgctgttc ttcccgatcg agcgcgcgca cgaggtgctt catgcgtggc gcgagtgggt
     9241 caagaacgtg cccgacgagg tcacctcggt cggccgcctc ctgcgcttcc cgccgctcga
     9301 ggagatccct gagccggtgc gcggcggcaa gttcgccgtg atcgaggcca cgatgctggt
     9361 cgacgaggct gaggccgcgg acctgctcgc gccgctgcgc gcgctcggcc cggcgatgga
     9421 caccttcgcg accatcccgg tcaccgagct gcagcacctg cacatggacc cgccgcaccc
     9481 ggtcccgggc aagggcgacg ggctgctgct gcgcgatctg cccgcgaagg cgctcgactc
     9541 gctgctggag gtcacggggc ccggcgtcga gtcgccgctc ctgagcgtcg agattcgcca
     9601 catcggcggc gcggccggca ggcccgaccc gaacggcggc gcgctcgact cgatcgacgc
     9661 gccgttcgtc atgttcgccg tcgggatgac gcccgtgccc gagatggtcg aggcggtcga
     9721 ggcgcacgtg aacatcgcga agcgcgcgct cggggcatgg gaccgcggtc tcatgtacct
     9781 gaactttgcg gaacacaaga tcgacccgag ccggctctgg tcgcccgccg cgtacctgcg
     9841 cctgcggaag atccgggcgg cctacgaccc ggacgagctg ttccgcgcga accacccggt
     9901 gcctccgttc ccggtggcag cggagtagtc ccagcaatac aaagctaggt ccgagctcca
     9961 gcccccgcct cgcagcgtcc gcgaggcggg ggctttcgca acttctggcc gtgtgcgcag
    10021 ttctcttccg tgcgggcccg gatggcccgc acaaacttcg ttcacgggga gacgccttgc
    10081 gtaaatacct cagcgtcacg ctcttgctcg ccgccgcttc gcttgcggca cctgccgccg
    10141 cttcggcgtc ggcgaccgtg ctcggcacga cttcctcgag cctcggctcg ccggccctag
    10201 cgggcgaccg ggtcgtcgcg acggtgtttc gcagcgcgaa cctcgagcag ctgtactcgt
    10261 tcgcgccggg gacgcctgcg aagcggctgc tcacgaaccg gacgcgtttc gagggcgact
    10321 tcgcggtcct cggctacggc gcctcggcgc agcgcgtcgc ggccgttcgg gttggcgagg
    10381 cgagcccggg aaacgctcag ttcttcagtg ggccaccgct cggcccgctc tcgcaa
//
LOCUS       DASVXS010000140        14681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_179918, whole genome shotgun sequence.
ACCESSION   DASVXS010000140 DASVXS010000000
VERSION     DASVXS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14681
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179918"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF752_07655"
     CDS             complement(<1..408)
                     /locus_tag="VF752_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855462.1"
                     /translation="MSSPLIVSAVDVRRRFGEGDAAVDALDGVTVGFPKGQFTAIMGP
                     SGSGKSTLMHILAGLDRPTAGSVAVDGVELGELDDKKLTQLRRDKFGFIFQFFNLLPV
                     LTAEENVTLPLRIAGEKVDRDWLEQLLATVGLTD"
     gene            complement(546..1190)
                     /locus_tag="VF752_07660"
     CDS             complement(546..1190)
                     /locus_tag="VF752_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855463.1"
                     /translation="MRVVVAEDSVLLREGVVRLLEEAQFEVVAQAGDADDLLRKVAAH
                     KPDVAIVDVRMPPTNTDDGLRAAQKIRADHPSTGVLVLSQYVEEGYALELLAENAEGV
                     GYLLKDRIADIERFTDSVKRVAEGGSALDPEVVAQLLGRRRREDPLEALTPREREVLG
                     LMAEGRSNNAIAEQLVVTERAVEKHVTSIFGKLGLAPAAEDHRRVLAVLTYLQA"
     gene            complement(1181..2179)
                     /locus_tag="VF752_07665"
     CDS             complement(1181..2179)
                     /locus_tag="VF752_07665"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8855464.1"
                     /translation="MTARRARSTADRAEALDDLIARVTFAPARSDLRQALARVLGDRS
                     LRVVYWYERREHYVDRRGRTVELPEPGDPKRAWTPVVYEGRTVAAIVHEARLAGDKQV
                     ARAAAALGAVFEREGIEAQLRASLASARSARGRILEAEQKSRRRLERDLHDGAQQRLV
                     ALALELKLARSKIETDPAGAAEMLDRADEGLRHAIEEVREIARGIHPAVLTDRGLRPA
                     LTALVGRSPVPVELELEIGDRLPERVEAAIYYVVAEALTNAAKHAKASSVSVNVVEKN
                     DVALVQVKDDGVGGADMRYGTGLIGLADRVAALDGQLEVDSYPGEGTVIYAEIPCA"
     gene            complement(2176..2538)
                     /locus_tag="VF752_07670"
     CDS             complement(2176..2538)
                     /locus_tag="VF752_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855465.1"
                     /translation="MALRVLIVDDHPSFRASARLLLEAEGYDVVGESEDGMTGLMDAR
                     RLRPDIVLLDVQLPDIDGFDVAARLTGDENAPHVVLTSSRELSDYGPLVSRSGARGFV
                     PKSELSGEAIEALVPTVS"
     gene            2668..7062
                     /locus_tag="VF752_07675"
     CDS             2668..7062
                     /locus_tag="VF752_07675"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8855466.1"
                     /translation="MKMDPLQSFTPQVRDWFARAFADPTKAQTDAWPAIARGENVLVS
                     APTGSGKTLAAFLWGLDRLVANPLPEGERRTRLVYVSPLKALSYDIERNLRAPLRGIG
                     GDVSVAIRTGDTPQKERQQMLRTPPDVLITTPESLYLILTSQAREMLRDAEWVIVDEI
                     HAVAQTKRGAHLALTLERLEHLAERPLQRIGLSATQRPLEEVGRFLVGPQRECTVIDA
                     GIRKELDLKIQVPVENMTEPDSDGSDVTDLAGGEATRRSIWPAIYPELLELVQSHRST
                     IVFVNNRRGAERLAVRLNELAGEDGQPADIARAHHGSLAREERLVVEEMLKSGELPCL
                     VATSSLELGIDMGAVDLVLQVESPKSVSRGLQRIGRAGHNVGEVSKGRIFPKFRADLL
                     ESTVVARRMREGEIESTVVPRNPLDVLAQQIVAMVAPGDEWRVPELHDLVRRTYPYSE
                     LSRELLDNVLDMLDGRYPSEEFAELRPRIVWDRVQDVIRARKGSRQLAVTNAGTIPDR
                     GLFGVHLPDGRRVGELDEEMVYEARPGQTFMLGASTWRIEEITRDRVIVTPAPGAPGA
                     VPFWRGDGVGRPAELGRAIGEFSRWAVDRDPQELVDGFDLDEYAANNLVAFLREQREA
                     TRVVPSDRTIVIERFRDEIGDWRLCVLSPYGGRVHAAWGLALSARIRDQFGLEADAIW
                     SDDGIIVHLPDADEPPGTELVMVEPDEVEDMVVRELGASALFGARFRENAARALLIPR
                     AYPGKRTPLWQQRLKAQSLLEVAKRYSQFPIILETYRECLRDVLDVPGLVDLLTKLHS
                     RELSLVEAETQTASPFASSLLFDYVATYMYEGDTPNAERRAAALSLDRELLRELLGQE
                     ELRDLIDPDALAEVEADLQHVSERTRAVNADGMHDVLRAVGDLTVAEAQERAIVAVSA
                     NRMLEVLVSERRAARMRIGGEERYIAAEDAGLYRDALGSVPPGGLPATFLEPVEEPLS
                     RLARRYARTHGPFTTAEISARYGVDLGPTLRELERSGGLVRGELRPGGAEREWCDPEV
                     LRRLRRASLASLRKEVEPAEQRALARFLPSWQGVDVSPPGGAGIDRLREVLVPLQGLA
                     LTPSTWEQDVLPRRVGAYSASWMDALCAAGEIVWVGAGALGRSNGRVALYFRDDARWI
                     GTPSQRGEAPSSPVHDAIRERLERGAAFWSDLVTDVTEVDGRAIEPTELQEALWDLAW
                     AGEVTNDAFAPLRAPRLSLARADRQRGRRFARRRAGAAPQVQGRWSLTAPLFRDAPER
                     GPRMRAISELLLERYGIVTREMVLAEGIPGGFSTLYGELSNLETLGTARRGYFVEGLG
                     GAQFALGAAVERLRSLRTDEPAGPLVLAATDPANAYGASLPWPRREDDDSGRRPSRAP
                     GAYVVMLDAEPVLYVERGGKGLLSLKEPGDWLREALEALADHVRRGRLKRLAIERFDG
                     EPVVGTEMEELLIEVGFRQGPRRLTLTA"
     gene            7103..7921
                     /locus_tag="VF752_07680"
     CDS             7103..7921
                     /locus_tag="VF752_07680"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX8855467.1"
                     /translation="MPEGDTIHNAARRVGAALVGKPILSIETPQQRHRMDRWPERLEG
                     RAVHSVDARGKHLFLRFDGDLTLHSHLRMGGLWGVFRRGERWRRSPRRAWLVIRTPEH
                     DVVQFDGPVLELMTEGRTRFDQQLAGLGPDIVAEGEFDQAKFLRRLREDDQTRGFGEA
                     LLDQRNVAGIGNIWKCEACFLAGVDPWRPTSEVADDEALAAVRAARPLMQLSAERGGR
                     PVSWEPERPATRQENRFWVYNRAGLPCRKCGEPVQARGQGDDNRTTFWCPGCQS"
     gene            7909..8676
                     /locus_tag="VF752_07685"
     CDS             7909..8676
                     /locus_tag="VF752_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX8855468.1"
                     /translation="MPVLMTRAPHLPPLKRVGHKGADLVAPGNTIESFEAALEHGVHM
                     IEFDVLQMRDGSLVLAHDFRDAYSRETVTLEEGLDHFAGEAYADVELDVDLKLPGYER
                     QVGEALFERELGARSLISSTWEQSLDVLGEAAPGVRRGWSVPRARRDYLKSFLRPATM
                     AVAIYMRRRFPSRAAAMIRTGRCEAIMAHWLLASARLVDHVHAVGGQVYIWTVDDAVR
                     IRLLDAMGVDGVITNDPRLFDADTGQARRLADLADPA"
     gene            complement(8650..9177)
                     /locus_tag="VF752_07690"
     CDS             complement(8650..9177)
                     /locus_tag="VF752_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855469.1"
                     /translation="MRRLIVTAAVAALAVAFVVVPTGAEGAKSSKYLWATINICDTLK
                     HPNDMGVRASMPGDGTHKRMYMRFRAQFYNATKKRYENVQGSGLSGWIYAGSARYTFR
                     QAGFTFSFDRPAPGAEFKLRGVVSFEWRKKQRTRSGKRRWVVVRKATRVTKGGRAGVR
                     GADPPGFSSGVCQIR"
     gene            complement(9292..10527)
                     /gene="ilvA"
                     /locus_tag="VF752_07695"
     CDS             complement(9292..10527)
                     /gene="ilvA"
                     /locus_tag="VF752_07695"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX8855470.1"
                     /translation="MAGTATGEVVSFEDVEAADARIESHVRRTPLLSSGELTRRVGAK
                     VFVKAENLQLTGSFKVRGATNKLAQLSKEQLTAGVCAASAGNHAQGLAFAASQFGARA
                     VLFMPAGAPLAKIAAVHQYGGEVRFVDGTYDDAQAAAQEAAEAEGMTLVHAFDDPQVV
                     AGQGTIGLEIARDAPDVGLIVVPLGGGGLATGVAIAAKQLLPGVRVVGVQAAACAPYL
                     ESLKAHRPIGARSANTICDGIAVKEPGRLTLPLVERYIDGVVTVTDDEVAQAMVMLLE
                     RTKLVVEGAGAVGVAALMQGLVKAPEGGSVCAILSGGNVDASLLYECIRLGETAAGRR
                     MVLATVVPDRPGALAGLLKTVADLGANVVDVEHLRDGMDIHVRETAIQLVLQTRGPEH
                     DEEIMKAVEAEGFSVRLEH"
     gene            complement(10530..10925)
                     /locus_tag="VF752_07700"
     CDS             complement(10530..10925)
                     /locus_tag="VF752_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8855471.1"
                     /translation="MIGDRRTVHAETAPAAVGPYSHAVAAGGLLFCSGQIPLDPESGE
                     LIEGSAGDQARRCLDNLRAVCEAAGASLDDAVRIGVFLTDLAGDWAEVNDAYGEYFGD
                     DAPARAAVGVASLPKGARVEIEAVVALGS"
     gene            10971..11666
                     /locus_tag="VF752_07705"
     CDS             10971..11666
                     /locus_tag="VF752_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8855472.1"
                     /translation="MSEGDDTIRLLGRAPIFSGLTNDELAGIAQVAVPRTWSAGEVIF
                     REGDPGDTAYVIRDGVVRVTRQHPGGRVITLVELRPGDMFGELAMLVDETRSATAEAT
                     VDTRGLALLAGDLRRILIAHPEIALKMLEVFAGRLRGANERISRQSFQTVAGRVASVL
                     LAQAEQLQRDGQAGEENVLIPATRAEIAQLAGSSRESVSRFLAGLERAGVVTCGRGKV
                     VVHDPRTLRSYIY"
     gene            11632..12306
                     /locus_tag="VF752_07710"
     CDS             11632..12306
                     /locus_tag="VF752_07710"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX8855473.1"
                     /translation="MTPAPSEATSTEELSRRRAEMVERQLRARGIGDECVLRAMGEVP
                     RHLFVPEDAVKDAYRDGALRIGEGQTISQPWIVAAMASLLELEGDETVLEVGTGSGYA
                     AAVLSRCCHDVITIERHGSLAERSRQVLAELGYDNVEVRVGDGSRGAADRAPFGGISV
                     TATAMSEPPPALLEQLAPGAALVCPVNVGGEERLTRFRDGEVEPIVAVRFVPLIESEG
                     GAGTAS"
     gene            12353..12745
                     /locus_tag="VF752_07715"
     CDS             12353..12745
                     /locus_tag="VF752_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8855474.1"
                     /translation="MVVRRMRGQPYFAAVRVKDGTVLALPKGHIDPGESAAEAAEREV
                     REETGVTGTLVEKLDDIRYWYFRDGRRVLKVVSFFLFRYKSGSVEDHDEEVDSAEWVP
                     LADGPDMLSYRGEREIARAALNRSNSGR"
     gene            12758..13156
                     /locus_tag="VF752_07720"
     CDS             12758..13156
                     /locus_tag="VF752_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX8855475.1"
                     /translation="MYVLNFYSPLFIDQLKRGRKTATIRLGDKASKYKKGQVVLITVG
                     FQHQPRERIFEAVIDRVEVKRVSELSPRDIEHDNPEFRRLDETVHFLEQIYGRQVDKD
                     DLVTVVEFSAISDRPSELSDRMRGAAGTQN"
     gene            13271..13561
                     /gene="groES"
                     /locus_tag="VF752_07725"
     CDS             13271..13561
                     /gene="groES"
                     /locus_tag="VF752_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8855476.1"
                     /translation="MKLKPLGDRLIVKAVEEEETTASGIVLPDTAKEKPQRGKVVAVG
                     DGKVTDDNERIPLDVSEGDEVLYSKYGGTEIKVDGEDLLVLRESDVLAKVAG"
     gene            13592..>14681
                     /gene="groEL"
                     /locus_tag="VF752_07730"
     CDS             13592..>14681
                     /gene="groEL"
                     /locus_tag="VF752_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8855477.1"
                     /translation="MAHKELKYDQEARASLEAGVDAVANAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIAREIEVEDVFQNQGAQLVREVATATNDVAGDGTTTATLLAQIIVRQ
                     GLKNVAAGANPLALRRGIERAVDQVVENIRSQAKQVSGKEQIARVAAISAADDEIGDV
                     IADAIDKVGKDGVVNVEEGQTFGMDLGFTEGMQFDKGYISPYMVTDQERMEAVLEDPY
                     ILIANQKIGSVRDLLPVLEQVIQSGRPIVIIAEDVEGEALATLVVNKLRGTFTGVAVK
                     APGFGDRRKRMLEDIAILTGGEVITEEMGLKLENTQISQLGRARRVVVTKDTTTIIDG
                     GGDADAIKGRINQIKQEIENTDSDFDREK"
BASE COUNT         2238 a         5166 c         5122 g         2155 t
ORIGIN      
        1 gtcggtgagg ccgacggtgg cgagcagctg ctcgagccag tcgcggtcga ccttctcgcc
       61 ggcgatgcgc agcggcagcg tgacgttctc ctccgccgtg agcaccggga gcaggttgaa
      121 gaactggaag atgaagccga acttgtcgcg gcgaagctgg gtgagcttct tgtcgtcgag
      181 ctcaccgagc tcgacgccgt cgacggccac cgagcccgcg gtgggccggt cgagcccggc
      241 gaggatgtgc atgagtgtcg acttgccgga gccggacggc cccatgatgg ccgtgaactg
      301 gcccttcggg aagccgacgg tcacgccgtc gagagcatcc accgcggcgt cgccttcgcc
      361 aaaacgacgg cggacgtcta cggccgagac gatgagaggg gaagacaagt tgcgagtcct
      421 ttcgatccgg agcggggaca gaggagtgac gcgatggtgg cggatgggaa cccgaattcc
      481 actgggggga cccggcttct tgcgggtggg ggtacccgca ctgcactaat tgaagtgagc
      541 ccgactcagg cctggagata ggtgagcacc gcgaggacgc ggcggtgatc ttcggcggcg
      601 ggcgcgagac cgagcttgcc gaagatgctc gtcacgtgct tctccacggc ccgctcggtg
      661 accacgagct gctcagcgat cgcgttgttc gagcggccct cggccatcag accgagcacc
      721 tcgcgctcgc gcggcgtgag cgcctcgagc ggatcctcgc ggcggcgccg cccgagcagc
      781 tgggcgacca cctcggggtc gagcgcggag ccgccctcgg cgacacgctt cacgctgtcg
      841 gtgaagcgct cgatgtcggc gatcctgtcc ttcagtaggt agccgacacc ctccgcgttc
      901 tccgcgagca gctcgagggc gtagccctct tccacgtatt gggagagcac gagcacgccc
      961 gtgctcgggt gatcggcgcg gatcttctgg gccgcgcgca ggccgtcgtc ggtgttggtc
     1021 ggcggcatgc gcacgtccac gatcgccacg tcgggcttgt gtgcggcgac cttgcgcagc
     1081 aggtcgtcgg cgtcgcccgc ctgggccacc acctcgaact gcgcctcctc gagcaggcgc
     1141 accacgccct cgcgcaggag gaccgaatct tcagcgacga ctacgcgcat gggatctccg
     1201 catagataac agtcccctcg ccgggatagc tgtccacctc gagttgacca tccagagccg
     1261 cgactctatc cgcaagtccg ataagtccgg tgccatatct catgtcggcg ccgccgacgc
     1321 cgtcgtcctt cacctgtacc agcgccacgt cgttcttctc gacaacgttg acggataccg
     1381 aggacgcttt ggcgtgcttg gcagcgttcg tgagcgcctc ggcgaccacg tagtagatcg
     1441 cggcctccac ccgctcgggc aggcggtcgc cgatctcgag ctcgagttca accggcaccg
     1501 gcgaccggcc gaccagcgcc gtgagagcag gccgcagccc tcggtcggtg agcaccgccg
     1561 ggtggatgcc gcgcgcgatc tcgcgtacct cctcgatcgc gtgacgcagg ccctcgtccg
     1621 cgcggtcgag catctccgcg gcgccggccg ggtcggtctc gatcttcgag cgtgcgagct
     1681 tcagctcgag cgcaagcgcc accagccgct gctgcgcacc gtcgtgcagg tcgcgctcga
     1741 ggcggcgccg cgacttctgc tccgcctcga ggatccggcc gcgcgccgag cgcgcggagg
     1801 ccagagacgc acgcagctga gcctcgattc cctcgcgctc gaagacggca ccgagcgccg
     1861 ccgcggcgcg ggccacctgc ttgtcgcccg ccagacgcgc ctcgtgcacg atcgcggcca
     1921 ccgtacggcc ctcgtacacg accggcgtcc aggcgcgctt cggatcgccg ggctcgggca
     1981 gctcgaccgt gcgaccgcgg cggtccacgt agtgctccct gcgctcgtac cagtagacga
     2041 cgcgcagcga gcggtcgccc agcacgcgcg ccagcgcctg gcgcaggtcg ctccgggcgg
     2101 gcgcgaaggt gacgcgcgcg attagatcgt cgagcgcctc cgcgcggtca gctgtcgaac
     2161 gggcgcggcg cgcggtcacg acaccgtcgg aacgagcgcc tcgatcgcct cgccgctgag
     2221 ctcggacttc ggaacgaagc cacgcgcccc gctgcgcgac acgagcggcc cgtagtcgga
     2281 cagctcgcgg ctcgaggtga gaacgacgtg cggcgcgttt tcgtcgccgg tgagccgggc
     2341 ggccacatcg aagccgtcga tgtccggcag ctgcacgtcg agcaggacga tgtcgggccg
     2401 gaggcgtcgt gcgtccatca gccccgtcat tccgtcctct gattccccca ccacgtcgta
     2461 gccctctgct tccagcagga gccgagcgga cgcccgaaaa cttggatggt cgtccacgat
     2521 gagaactctg agagccactg ccggaattgt gaagtgatgc ggaatttgcg cccatacggg
     2581 tagccacacc ccccttggcc cttgagcgga ggacagcctg ccccctctgg gggatacgcc
     2641 cctcggcgca gtacgctggt gggtcgcatg aaaatggatc cgcttcagag cttcacccca
     2701 caggttcgcg attggttcgc gcgggctttc gcggatccga cgaaggcgca gaccgacgcc
     2761 tggcccgcca tcgcgcgcgg tgagaacgtg ctggtgtcgg cgccgacggg ctcgggcaag
     2821 acgctcgcgg cattcctgtg ggggctcgac cggctggtcg cgaacccgct tcccgagggc
     2881 gagcggcgca cgcggctcgt gtacgtgtcg ccgctgaagg cgctctcgta tgacatcgag
     2941 cgcaacctgc gggcgccgct gcgcgggatc ggcggcgacg tctccgtcgc gatcaggacc
     3001 ggtgacacgc cgcagaagga gcgccagcag atgctgcgta cgccgccgga cgtgctcatc
     3061 accacgccgg agtcgctcta cctgatcctc acctcgcagg cgcgcgagat gctgcgcgac
     3121 gcggagtggg tgatcgtgga cgagatccac gcggtcgccc agaccaagcg cggcgcgcac
     3181 ttggcgctca cgctcgagcg gctcgagcac ctcgccgagc gcccgctcca gcgcatcggc
     3241 ctctcggcca cacagcgacc gctcgaggag gtcggccgct tcctcgtcgg cccgcagcgc
     3301 gagtgcacgg tgatcgacgc cggcatacgc aaggagctcg acctgaagat ccaggtgccg
     3361 gtcgagaaca tgaccgagcc ggactccgac ggctctgacg tcacggacct agcgggcggg
     3421 gaggcgacgc gccggtcgat ctggccggcg atctacccgg agctgctcga gctggtgcag
     3481 tcgcaccgtt ccacgatcgt cttcgtgaac aaccgccgcg gcgcggagcg cctggccgtg
     3541 cggctgaacg agctggccgg ggaggacggc cagcctgccg acatcgcccg cgcccaccac
     3601 ggctcgctcg cgcgcgagga gcgcctcgtg gtcgaggaga tgctcaagtc cggcgagctg
     3661 ccgtgcctgg tggccacctc gtcgctcgag ctcgggatcg acatgggcgc cgtcgacctc
     3721 gtgctgcagg tcgagtcgcc gaaatcggtg agccgcgggc tgcagcgcat cgggcgggcc
     3781 ggccacaacg tgggcgaggt ctcgaagggc cgcatcttcc cgaagttccg cgccgacctg
     3841 ctcgagtcga ccgtggtcgc ccggcgcatg cgcgagggcg agatcgagtc gacggtcgtg
     3901 ccgcgcaacc cgctcgacgt gctcgcgcag cagatcgttg cgatggtcgc ccctggggac
     3961 gagtggcgcg tgcccgagct gcacgacctc gtccggcgca cctatcccta ctccgagctc
     4021 tcgcgtgagc tgctcgacaa cgtgctcgac atgctcgacg ggcggtatcc gtccgaggag
     4081 ttcgccgagc tgcggccgcg gatcgtctgg gaccgcgtgc aggacgtgat ccgggcgcga
     4141 aagggctcgc ggcagctggc cgtgaccaac gcgggcacga tccccgaccg cgggctcttc
     4201 ggcgtgcatc tgcccgacgg gcggcgagtc ggtgagctcg acgaggagat ggtctacgag
     4261 gcgcggcccg gccagacctt catgctcggc gcctcgacct ggcgcatcga ggagatcacg
     4321 cgcgatcgcg tgatcgtgac gcccgccccc ggcgcgcccg gcgccgtgcc gttctggcgg
     4381 ggggacggcg tgggccggcc agcggagctc ggccgggcga tcggcgagtt ctcgcgatgg
     4441 gcggttgacc gggacccgca ggagctcgtc gacggcttcg acctggacga gtacgccgcg
     4501 aacaacctcg tcgccttctt gcgcgagcag cgcgaggcga cgcgcgtggt gccctcggac
     4561 aggacgatcg tgatcgagcg cttccgcgac gagatcggcg actggcgact ttgcgtgctg
     4621 tcgccgtacg gcggccgcgt ccacgccgcc tggggcctcg cgctgtccgc gcgcatccgc
     4681 gaccagttcg gactcgaggc cgacgccatc tggtcggacg acggcatcat cgtccacctg
     4741 cccgacgccg acgagccgcc cggcacggag ctcgtcatgg tcgagccgga cgaggtggag
     4801 gacatggtcg tgcgcgagct cggcgcctcg gccctgttcg gcgcgcgctt ccgcgagaac
     4861 gcggctcgtg cgctgctgat cccgcgcgcg tatcccggca agcgcacgcc gctgtggcag
     4921 cagcggctga aggcgcagtc gctgctcgag gtggcgaagc gctacagcca gttcccgatc
     4981 atcctcgaga cctaccgcga gtgcctgcgc gacgtgctcg acgtgccggg ccttgtggac
     5041 ctgctgacga agctgcacag ccgcgagctg tcactcgtcg aggccgagac gcagacggcg
     5101 tcgccgttcg cgtcctcgct gctgttcgac tacgtggcca cgtacatgta cgagggcgac
     5161 acgccgaacg ccgagcgccg cgcggccgcc ctctcgctag accgcgagct gctgcgcgag
     5221 ctgctcggcc aggaggagct gcgcgacctg atcgacccgg acgcgctcgc cgaggtcgag
     5281 gccgacctgc aacacgtttc cgagcgcacg cgcgcggtca acgcggacgg catgcacgac
     5341 gtgctgcggg cggtcggcga cctgacagtg gcggaggcgc aggagcgggc gatcgtggcg
     5401 gtgtcggcca accgcatgct cgaggtgctc gtgagcgagc gccgcgccgc aaggatgcgg
     5461 atcggcggcg aggagcgcta tatcgccgcc gaagacgcgg ggctctaccg cgatgcgctc
     5521 ggctccgtac cgcctggcgg cctgcccgcc acgttcctcg aaccggtcga ggagccgctc
     5581 tcgcgcctcg cacgccggta cgcgcgtacg cacgggccat tcaccaccgc cgagatctcc
     5641 gcgcgctacg gcgtcgacct cggacccacg ctccgggagc tcgagcgaag cggcgggctg
     5701 gttcgaggcg agctgcgccc aggtggcgcg gagcgcgagt ggtgcgatcc cgaggtgctg
     5761 cgccgcctgc gccgcgcgtc gctcgcgtca cttcgcaagg aggtcgagcc cgccgagcag
     5821 cgtgcgctgg cgcgcttcct gccctcctgg cagggcgtgg acgtgtcgcc tccgggcggc
     5881 gccgggatag accgcctccg cgaggttctg gtgccactcc agggacttgc gctcacgccc
     5941 tccacctggg agcaagacgt gctgccgcga cgcgtgggcg cgtactcggc ttcatggatg
     6001 gacgcgctct gcgcggccgg cgagatcgtg tgggtcggcg ccggggcgct cggtcgctcg
     6061 aacggcaggg tggccctcta cttccgtgac gacgcccggt ggatcggcac accgtcacag
     6121 cgcggcgagg cgccctcatc gcccgtgcac gacgcgattc gcgagcggct cgagcgcggc
     6181 gcggcgttct ggtccgacct ggtcacagac gtgaccgagg tggacgggcg ggccatcgaa
     6241 ccaaccgaac tgcaggaggc gctctgggat ctcgcttggg ccggagaggt gacgaatgac
     6301 gccttcgcgc cgctgcgcgc gccgcggctg tcgcttgccc gcgccgaccg ccagcgcggc
     6361 cggcgctttg cgcgccgccg agccggcgcc gcgccgcagg tgcagggccg ctggtcgctc
     6421 accgcgcccc tgttccgtga cgcgcccgag cgtggcccgc gcatgcgcgc gatctcggaa
     6481 ttgctgctcg agcgctacgg catcgttacg cgcgagatgg tcctagccga ggggattccc
     6541 ggcggcttct ccaccctcta cggcgagctg tccaatctcg agacgctcgg caccgcgcgc
     6601 cgcggctact tcgtcgaggg cctgggcggc gcgcagttcg cgctcggcgc ggcagtggag
     6661 cggttgcgct cgcttcgcac cgacgagccc gccggcccgc tcgtgctcgc cgcgaccgac
     6721 cccgcgaacg catacggcgc ctcgctcccg tggccgcgcc gggaagacga cgactcgggc
     6781 cgccgcccca gccgggcgcc cggcgcctac gtggtgatgc tcgacgcgga gcccgtcctc
     6841 tacgtggaac ggggcggcaa gggcctgctc agcctgaaag agccaggcga ctggttgcgc
     6901 gaggcgctcg aggcgctcgc cgaccacgtg cgacgcggtc ggctcaagcg gctggcgatc
     6961 gagcgcttcg acggcgagcc ggtcgtggga accgagatgg aggagctgct gatcgaggtc
     7021 ggcttccgcc agggcccgcg gcgcctgact ctgacggcat agctgtggcg caccccgtcg
     7081 gctcgttcgt aacatcgaac gaatgccgga aggggacacg attcacaacg ccgctcggcg
     7141 cgtgggggct gccctggtgg ggaagccgat cctctcgatc gagacgcctc agcaacgcca
     7201 ccgcatggac cgctggcccg agcgcctcga gggaagagcc gtgcacagcg tcgacgcgcg
     7261 gggcaagcac ctcttcctgc ggttcgacgg cgacctgacg ctccactcgc acctccgcat
     7321 gggcggcttg tggggtgtgt tccgacgcgg cgagcgctgg cgccggtctc cgcggcgcgc
     7381 ctggctcgtg atccgcacgc ccgagcacga cgtcgtgcag ttcgacgggc cggtgctcga
     7441 gctgatgacg gaaggccgca cgcgcttcga ccagcagctc gccggcctcg gcccggacat
     7501 cgtggccgag ggcgagttcg accaggcgaa gttccttcgc cgcctgcgcg aggacgacca
     7561 gacgcgcggc ttcggggagg cactgctcga ccagcgcaac gtggccggca tcggcaacat
     7621 ctggaagtgc gaggcctgct tcctcgccgg cgtcgatccg tggcgtccca cgtccgaggt
     7681 ggcggacgac gaagccctcg cagccgtgcg cgccgcgcgg ccgctcatgc agctgtctgc
     7741 cgagcgcggc ggccggcccg tcagctggga gcccgagcgt ccggccaccc gacaggagaa
     7801 ccgcttttgg gtctataacc gcgcaggcct gccctgccgg aaatgcggcg agccggttca
     7861 ggcgcgcggc cagggcgatg acaaccgcac caccttctgg tgcccggggt gccagtcctg
     7921 atgacgcgcg cgccgcacct gccgccgctc aagcgagtcg ggcacaaggg cgccgacctg
     7981 gtcgcccctg gcaacacgat cgagagcttc gaggccgcgc tcgagcacgg cgtgcacatg
     8041 atcgagttcg acgtgctgca gatgcgcgac ggctcgctcg tactcgcgca cgacttccgc
     8101 gacgcgtaca gccgcgagac cgtgacgctc gaggagggtc tcgaccactt cgcgggcgag
     8161 gcatatgcgg acgtggagct cgacgtggac ctcaagctgc ccggctacga gcgccaagtc
     8221 ggcgaggcgc ttttcgagcg cgagctcggc gcgcgttcgc tcatctcgtc cacatgggag
     8281 cagagcctcg acgtgctcgg ggaggctgcg cctggggtgc ggcgcggctg gtcggtaccg
     8341 cgcgcgcggc gcgactacct caagagcttc ctgcgtccgg cgacgatggc ggtcgccatc
     8401 tatatgcgcc ggcgcttccc ctcgcgagca gcggcgatga tccgcaccgg ccgctgcgag
     8461 gcgatcatgg cccactggct gctcgcaagc gcccggcttg tggaccatgt gcacgcagtc
     8521 gggggccagg tctacatctg gacggtcgat gacgccgtgc gcatccgcct gctcgacgcg
     8581 atgggggtgg acggcgtgat cacaaacgac ccgcgtctgt tcgacgccga taccggccag
     8641 gcgcggcggc tagcggatct ggcagacccc gcttgagaac ccgggcgggt cggcgccgcg
     8701 cacgcccgct cggccgccct tcgtcacccg ggtcgccttg cgcacgacca cccagcggcg
     8761 cttgccgctg cgcgtccgct gcttcttccg ccactcgaag ctcaccaccc cgcgcagctt
     8821 gaactcagcg cccggcgcgg ggcgatcgaa cgagaaggtg aagccggctt ggcggaacgt
     8881 gtagcgcgcc gagccggcgt agatccaacc cgaaagcccg ctgccctgaa cgttctcgta
     8941 gcgcttcttg gtggcgttgt agaactgcgc gcggaagcgc atgtacatgc gcttgtgggt
     9001 gccgtcgccg ggcatgctcg cgcgcacgcc catgtcgttg gggtgcttga gcgtgtcgca
     9061 gatgttgatc gtcgcccaga ggtacttcga cgacttcgcg ccttcagccc cggtggggac
     9121 gacgacgaac gcgacggcca aggcggccac cgcggcggtg acgatcaagc gcctcacgcc
     9181 accaatgatg ctcccgatgg gaaaaagttg cggaccccgt ggacgcagat ccggcgctac
     9241 tgaccgcagg cagtggcgct accagccgta gccggaccgg agtccacttg cctagtgctc
     9301 gaggcggacg gagaagccct ctgcctcgac agccttcatg atctcctcat cgtgctccgg
     9361 accgcgcgtc tggagcacca gctggatcgc cgtctcgcgc acgtggatat ccatgccgtc
     9421 gcgcaggtgc tcgacgtcga cgacgttggc gcccaggtcg gccaccgtct tgagcagccc
     9481 ggccagcgcg ccggggcggt cgggcacgac ggtggcgaga accatccgcc gccccgcggc
     9541 ggtctcaccc aggcgaatgc actcgtaaag cagcgacgca tcgacgttgc caccggagag
     9601 gatcgcgcag acgctcccgc cctcgggcgc cttcaccagt ccttgcatga gcgcggccac
     9661 gcccaccgcg ccagctcctt ccaccaccag cttcgtgcgc tcgagcagca tcaccatcgc
     9721 ttgggcaacc tcgtcatcgg tcacggtcac cacgccgtcg atgtaacgct ccacgagcgg
     9781 cagcgtgagt cggcccggct ccttcaccgc gatgccgtcg cagatcgtgt tcgccgagcg
     9841 cgcgccgatc ggccgatgtg ccttgagcga ctccaggtac ggcgcgcacg ccgcagcctg
     9901 cacgccaacc acgcgcacgc cgggcagcag ctgcttggcc gcgattgcca cgccggtggc
     9961 gagcccgccg ccgccgagcg gcaccacgat caggccaacg tccggggcat cgcgcgcgat
    10021 ctccagcccg atcgtgccct gacccgccac cacctgcgga tcgtcgaagg cgtgcacgag
    10081 cgtcatgccc tccgcctcgg ccgcctcctg tgcggcggcc tgggcgtcgt cgtaggtgcc
    10141 atccacgaag cggacttcgc cgccgtactg atgcaccgcg gcgatcttcg cgagcggcgc
    10201 gcccgccggc atgaacagga cggctcgcgc gccaaactgc gaggcggcga aggcgaggcc
    10261 ctgtgcgtgg ttacccgcgc ttgcggcgca cacgccggcc gtgagctgct ccttcgacag
    10321 ctgcgccagc ttgttggtgg caccgcgcac cttgaacgag ccagtgagct gaaggttctc
    10381 ggccttcacg aacaccttcg cgccaacgcg ccgcgtcagc tcgccagacg agagcagcgg
    10441 agtgcgccgg acgtgcgact cgatcctcgc gtcggccgcc tcgacgtcct cgaaggagac
    10501 gacctccccc gtcgccgtcc ccgccaccgc tagctcccca gcgcgaccac ggcttctatc
    10561 tccacgcggg cgcccttcgg aagggaggcc acgccgaccg ccgcgcgcgc cggcgcgtcg
    10621 tcgccgaagt actcgccgta ggcatcgttc acctcggccc agtcgccggc gaggtcagtg
    10681 aggaacacgc cgatcctgac ggcgtcgtcg aggctcgcgc cggccgcctc gcagacggcg
    10741 cggaggttgt cgagacagcg gcgcgcctgg tcgccggcgc tgccctcgat cagctcgccg
    10801 ctctccggat cgagcggaat ttgcccggag cagaacagca gccccccggc cgcgaccgcg
    10861 tgcgagtacg gccccacagc cgcaggagcc gtctccgcat gcaccgttcg cctgtccccg
    10921 atcacgcccg ggagcctact cctggacccg cctgggatca tctgcccttc atgagcgagg
    10981 gggatgacac gattcggctg ctcggccgcg cgccgatctt ctccggattg accaacgacg
    11041 agctcgccgg gatcgcccaa gtggctgtcc cgcgcacctg gtcggcaggc gaggtgatct
    11101 ttcgcgaggg cgatccgggc gacaccgcct acgtgatccg cgacggcgtc gtccgcgtca
    11161 cgcgccaaca cccgggcggg cgcgtgatca cactggtgga gctgcgcccg ggcgacatgt
    11221 tcggggagct tgcgatgctc gtcgacgaga cgcgctcggc caccgcggag gccaccgtgg
    11281 acacgcgcgg gctcgcgctg ctcgccggcg acctccgccg catcctcatc gcccaccccg
    11341 aaatcgcgct gaagatgctc gaggtgttcg cgggtcgcct gcgcggcgcc aacgagcgca
    11401 tctcgcgcca gtcgttccag accgtcgccg gccgcgtcgc cagcgtgctg ctcgcgcagg
    11461 ccgagcagct gcagcgcgac ggccaggccg gcgaggagaa cgtgctcatc cccgccacgc
    11521 gcgcggagat cgcgcagctc gcgggctcgt cgcgcgagag cgtttcccgc ttcctcgcgg
    11581 gactcgaacg tgccggcgtc gtcacctgcg gccgcgggaa ggtggttgtc catgaccccc
    11641 gcaccctccg aagctacatc tactgaggag ctcagccggc gccgtgccga gatggtggag
    11701 cgccagctgc gcgcacgcgg gatcggcgac gagtgcgtgc tccgcgcgat gggcgaggtg
    11761 ccgcgccacc tgttcgtgcc ggaggacgcc gtgaaagacg cctaccgcga tggcgcgctg
    11821 cggatcggcg agggccagac gatctcccag ccgtggatcg tcgcggcgat ggcgtcgctg
    11881 ctcgagctcg agggcgatga gaccgtgctc gaggtcggca ccgggtcggg ctacgcagcc
    11941 gccgttctct cccgctgctg ccacgacgtg atcacgatcg agcgccacgg gagcctcgcg
    12001 gagcgctcac ggcaggtgct tgcggagctc ggctacgaca acgtcgaggt gcgggtgggc
    12061 gacggcagcc gcggggcggc ggacagggcg ccgtttggcg gcatctcggt aaccgccacc
    12121 gccatgagcg agccgccgcc ggcgctgctc gagcagctcg ccccaggcgc ggcgctcgtg
    12181 tgccccgtga acgtgggcgg cgaggagcgg ctcacccgct tccgcgatgg cgaggtcgag
    12241 ccgatcgtgg ccgtccgttt cgtgccgctg atcgagtcag aaggcggggc cggaactgcc
    12301 tcgtaggcct ccctccggcg gcgggctgct gcgcgagttc tccgccggag gcgtggtggt
    12361 ccggcgcatg cgcgggcagc cctacttcgc cgccgtccgc gtgaaggacg gcaccgtgct
    12421 cgcgctgccc aaggggcaca tcgaccccgg cgagtcggcc gccgaggccg ccgagcggga
    12481 ggtgcgcgag gagaccggcg tgactgggac gctggtcgag aagctggacg acatccgcta
    12541 ctggtatttc cgcgacggcc ggcgcgtgct gaaggtcgtg agcttcttcc tcttcaggta
    12601 caaaagcggc tccgtggagg accacgacga ggaggtcgac tccgccgaat gggtcccgct
    12661 cgcggatggc ccggacatgc tttcctaccg tggcgagcgc gagatcgcgc gcgcagccct
    12721 gaacaggtcg aactcgggga ggtaggctcc cccagccgtg tacgtactca acttctattc
    12781 accgctcttc atcgatcagc tgaagcgggg ccgcaagacg gccacgatcc gcctcggcga
    12841 caaggcgtcg aagtacaaga agggccaggt cgttctgatc acggtcggct tccagcacca
    12901 gccgcgcgag cgcatcttcg aggccgtgat cgaccgcgtc gaggtgaagc gcgtgagcga
    12961 gctctcgccg cgcgacatcg agcacgacaa ccccgagttc cgccgtctcg acgagaccgt
    13021 ccacttcctc gagcagatct acggtcgcca ggttgacaag gacgacctcg tcaccgtcgt
    13081 ggagttctcg gcgatctccg accgcccaag cgagctctca gatcgtatgc gcggcgccgc
    13141 cggcactcag aactgagtta ttttcgcctg gcaagcggga ttttcaggtg tttttggcac
    13201 tcttcctgct agagtgccaa agccgtagga cccctctttt caggactcac atcaacggag
    13261 gttcagcctg atgaagctca agcctcttgg cgatcgcctg atcgtcaagg cggtcgagga
    13321 ggaggagacg accgcaagcg gcatcgtcct tcccgacacc gccaaggaga agccgcagcg
    13381 cggcaaggtc gtcgcggtcg gcgacggaaa ggtcaccgac gacaacgagc gcatcccgct
    13441 cgacgtgtcc gagggcgacg aggtcctcta cagcaagtac ggcggcaccg aaatcaaggt
    13501 cgacggtgag gatctgctcg tgctccgcga gtcggacgtc ctcgccaagg tcgccggctg
    13561 atcccctcag gaaatcaacg gaggaatagg tatggctcat aaggagctga agtacgacca
    13621 ggaggctcga gcgtcgctcg aggccggcgt cgacgccgta gccaacgcgg tgaaggtgac
    13681 gctcggcccg aagggacgct acgtcgtcct ggacaagaag ttcggcgccc cgaccatcac
    13741 gaacgacggt gtgaccatcg cgcgtgagat cgaggtcgag gacgtcttcc agaaccaggg
    13801 cgcccagctc gtgcgtgagg tcgccacggc gaccaacgac gtcgcgggcg acggcaccac
    13861 cacggcgacg ctgctcgccc agatcatcgt ccggcagggc ctcaagaacg tggccgccgg
    13921 cgcgaacccg ctggctcttc gccgcggcat cgagcgcgcg gtcgaccagg tcgtggagaa
    13981 catccgcagc caggcgaagc aggtctccgg caaggagcag atcgctcgcg tggcggccat
    14041 ctccgccgct gacgacgaga tcggcgacgt gatcgccgac gcgatcgaca aggtcggcaa
    14101 ggacggcgtc gtgaacgtcg aggagggcca gacgttcggc atggacctcg ggttcaccga
    14161 gggcatgcag ttcgataagg gctacatctc gccgtacatg gtcacggacc aggagcgcat
    14221 ggaggcggtc ctcgaggatc cctacatcct catcgccaac cagaagatcg gctccgtccg
    14281 tgacctgctg cccgtgctcg agcaggtgat ccagtcgggc cgcccgatcg tgatcatcgc
    14341 cgaggacgtc gagggcgagg ctctcgccac gctcgtcgtg aacaagctcc gcggcacctt
    14401 caccggcgta gcggtcaagg ccccgggctt cggcgatcgc cgcaagcgca tgctcgagga
    14461 catcgcgatc ctcacgggcg gcgaggtgat caccgaggag atgggcctca agctcgagaa
    14521 cacccagatc tcgcagctgg gccgtgcccg tcgcgtcgtg gtgaccaagg acaccaccac
    14581 gatcatcgac ggcgggggtg atgcggacgc catcaagggc cgcatcaacc agatcaagca
    14641 ggagatcgag aacaccgact cggacttcga ccgcgagaag c
//
LOCUS       DASVXS010000141        11797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_533009, whole genome shotgun sequence.
ACCESSION   DASVXS010000141 DASVXS010000000
VERSION     DASVXS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533009"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..773)
                     /gene="rpsC"
                     /locus_tag="VF752_07735"
                     /pseudo
     CDS             complement(150..773)
                     /gene="rpsC"
                     /locus_tag="VF752_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(784..1119)
                     /gene="rplV"
                     /locus_tag="VF752_07740"
     CDS             complement(784..1119)
                     /gene="rplV"
                     /locus_tag="VF752_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX8855478.1"
                     /translation="MVVRAQARYVRTAPRKARLVIDHIRGKSVDEARAILTHTPRAAA
                     RDVAKLLDSAIANAENNHELVADDLVINQAYVDEGPTLKRYRPRALGRATRIRKRTSH
                     LTITLSPKE"
     gene            complement(1482..1760)
                     /gene="rpsS"
                     /locus_tag="VF752_07745"
     CDS             complement(1482..1760)
                     /gene="rpsS"
                     /locus_tag="VF752_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX8855479.1"
                     /translation="MSRSSKKGPWIEERLIRRIEAMNESGSKQMVKTWSRTSTIFPEM
                     VGHTIAVHDGRKHVPVFVSESMVGHKLGEFAPTRLFRGHAGSDKARMR"
     gene            complement(1770..2609)
                     /gene="rplB"
                     /locus_tag="VF752_07750"
     CDS             complement(1770..2609)
                     /gene="rplB"
                     /locus_tag="VF752_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX8855480.1"
                     /translation="MPIKKYKPTSPGRRFATFADNAEVTKSEPEKSLVEGLNKSGGRN
                     SNGRITSRHRGGGAKRKYRVIDFKRRKDGIPARVAAIEYDPNRTAYIALLHYADGEKR
                     YILAPNRLRVGATVMSGTGADIAPGHALPLRNIPTGTTVHNVELTPGRGGQLGRSAGA
                     AVQLVAKEGDRATLRLPSGEMRMVHVDCRATVGSIGNAEHQNIDIGKAGRSRHLGKRP
                     QTRGTAMNPVDHPHGGGEGSTTPGRHPVTPWGVPTLGYPTRKKNKQSDRWIVRSRRRA
                     KRK"
     gene            complement(2622..2918)
                     /gene="rplW"
                     /locus_tag="VF752_07755"
     CDS             complement(2622..2918)
                     /gene="rplW"
                     /locus_tag="VF752_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX8855481.1"
                     /translation="MNPRSVIVRPIVSEKSYALLAANKYTFQVHDDAHKTQVRQAVEE
                     IFNVRVLDVRTMSVKSKPKRRGYTRGRTAEWKKAIVQLHPDDTIELFEGQEIGE"
     gene            complement(2915..3574)
                     /gene="rplD"
                     /locus_tag="VF752_07760"
     CDS             complement(2915..3574)
                     /gene="rplD"
                     /locus_tag="VF752_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX8855482.1"
                     /translation="MAAPKAAVLGKTTKANLDASVFGEPFHMALVHETVRAELNARRR
                     GTASTLSRGEVAMTGGKAFRQKGTGRARTGALSTPQRIGGGIAFGPKPRDYVVKVNRK
                     ARRKALRAALSVHAGRGTLAVVEAGKFDSPSAGQAAEALAGFGEGKTLVVLALEGEEN
                     CAKSFRNLRDVAAVLPASAVGVADIIGATRLVVSQAALDELTRLAQPVARDSGGEDGD
                     S"
     gene            complement(3574..4191)
                     /gene="rplC"
                     /locus_tag="VF752_07765"
     CDS             complement(3574..4191)
                     /gene="rplC"
                     /locus_tag="VF752_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX8855483.1"
                     /translation="MAALLGKKLGMTQVFQEDGRLERVTVVQAGPCFVTGIRTHERDG
                     YEAVQLGFGEVNEKRLTKAEAGHTKKAGVPAVRHLVEFRDEAGELQVGDTVGVDVFEK
                     GQTVKVSGISKGKGFQGTVKRHNFSRGPVTHGSHNVRAPGSIGASATPSRVFKGIRGP
                     GQMGNKRVTQKGLEVVDVVPDENLILLRGSVPGAKGSTVEIRTDS"
     gene            complement(4195..4509)
                     /gene="rpsJ"
                     /locus_tag="VF752_07770"
     CDS             complement(4195..4509)
                     /gene="rpsJ"
                     /locus_tag="VF752_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX8855484.1"
                     /translation="MTQNKIRIRLKAYDHSSIEQAAKEIVDTAQRTGASVSGPVPLPT
                     EKNVYCVIRSPFKDKDSREHFEIRTHKRLIDIHQPTPKTVDSLQRLDHLPAGVDIQIQ
                     LV"
     gene            complement(4602..5789)
                     /gene="tuf"
                     /locus_tag="VF752_07775"
     CDS             complement(4602..5789)
                     /gene="tuf"
                     /locus_tag="VF752_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX8855485.1"
                     /translation="MAKEKFERDKPHVNVGTIGHIDHGKTTLTAAITQVLAEKVGGQA
                     RSFAEIDNAPEEKERGITIATSHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKSDMVDDPELLELVEVEVRDLLSE
                     YEFPGDDIPFITGSALKALEGDEEQKGKILELAEALDSYIPEPERDLDKPFLMPVEDV
                     FSITGRGTVATGRIEQGIIKTGEEVELVGIHPETAKTVVTGVEMFRKILDEGRAGDNV
                     GCLLRGTKREEIERGQVLCKPGSITPHTKFKAEVYCLKKEEGGRHTPFFDGYRPQFYF
                     RTTDVTGVAHLPEGTEMVMPGDNVQMSIELIQPIAMDQGLRFAIREGGRTVGSGVVTE
                     IVE"
     gene            complement(5861..7969)
                     /gene="fusA"
                     /locus_tag="VF752_07780"
     CDS             complement(5861..7969)
                     /gene="fusA"
                     /locus_tag="VF752_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8855486.1"
                     /translation="MPRKVSLDHTRNIGIMAHIDAGKTTTTERILFYTGRTHKLGEVH
                     EGAAVMDWMEQEQERGITITSAATTAFWRDYRINIIDTPGHVDFTVEVERSLRVLDGA
                     IALFDSVAGVEPQSETVWRQADKYRVPRIAYINKMDRIGANFAQGVQTMIDRLGANPI
                     PIQLPIGAEADFQGIIDLVTERAIIYKDDLGREWDEIDIPADQVDRAHEARTHLIEAI
                     ADHDEALMETYLADEPIPVDRLKAAIRKATLDISITPVLCGSSFKNKGVQPLLDAVID
                     YLPSPLDVPPVDGLEPVKGEEEGRPATREASDDAPFSALAFKVMSDPFVGKLTYFRVY
                     SGKLGAGSRVLNASNGKTERAGRILMMHANDREEQEEIYAGDIAAVVGFKQTSTGDTL
                     SAPDAPVLLETIEFPEPVVHVAIEPKTKVDQEKMSAALARLAEEDPTFQVRTDEETGQ
                     TVISGMGELHLEVLVDRMKREFKVEAAVGRPQVAYRETVRGTAQKVEGRFVRQTGGSG
                     QYGIVYIDVEPAPGEGFDFVNKIKGGSIPSEFIPAVEDGIEEALETGVRAGYPMVDVR
                     VTLTDGKYHDTDSSELAFKIAGSLALKEGAKRAKPVLLEPIMAVEVVTPEEFLGDVIG
                     DLSRRRGRVEGQDRRGNALAVQSSVPLSEMFGYATDLRSNTQGRATYTMQFDRYEEVP
                     GNIAEDIVEHRTGEPVGASA"
     gene            8199..8651
                     /locus_tag="VF752_07785"
     CDS             8199..8651
                     /locus_tag="VF752_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855487.1"
                     /translation="MHRAQPAVLILGLAGGFLLAITDFLTVVHVEVVTASCQDLATPS
                     LADSCVSKGSEQHSWALLVLGLLALAMTYGAAAGRSRPAAAALLLIGVGALAVFFAVD
                     LPDVSKTGQIGLNFANAQAKAGAGFWTELVGACLLVFAGAVGLTRARR"
     gene            8682..10301
                     /locus_tag="VF752_07790"
     CDS             8682..10301
                     /locus_tag="VF752_07790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX8855488.1"
                     /translation="MAERTIDSVARDAFGFEALRPGQREAIDAVLTGRDTLAVMSTGS
                     GKSAIYQIAGMLMEGATLVISPLIALQRDQVEAIDDQLPGEAAGVNSTVARSEREEAF
                     EELEEGELEFLFLAPEQLANPEVLENLREARPSLLVVDEAHCISEWGHDFRPDYLRLG
                     ALVDELGHPTVLGLTATASPPVRDEIVERLHMRDPDVIVRGFDRPNIHLAVERFHEEE
                     HKRRALVEAIEAAERPGIVYAATRAIAEELAEELAGKGIAACAYHAGMNATERDRIQE
                     EFMDDAFEVIVATTAFGMGIDKPNIRFVFHHAVADSVDSYYQEIGRAGRDGEPAQAKL
                     FYRTEDLGLRRFFAGAGQVDLDEIAHVADAVRRHGGPVDPVELKDELALSQTKLITAV
                     NRLEEAGALHTRPTGEVELVPDAPPPQQFVEEAAKREHDRHEFDRSRVEMMRAYAEHR
                     NCRRQFILSYFGEDLPEPCGNCDNDEAGLTRESNGDEPFPVGTRVVHRQWGGGLVQRY
                     EDDEVVVLFDSVGYKTLGVPLVLERDLLAPE"
     gene            10318..10881
                     /locus_tag="VF752_07795"
     CDS             10318..10881
                     /locus_tag="VF752_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8855489.1"
                     /translation="MAPALIPERIETERLALRRNAPADLDAFVAIWRDPAVWRALMGG
                     GEPDLEFAAERHARQVEHWQREGFGLYLVEIAGEVAGWCGPWHPDFIPSLSSEIEIGW
                     TLRHRFWGFGFATEAAAIAAGIAFERLAPRRTISLIAKSNRRSREVAKRLSMSIAGEA
                     EIPDSGITLDVWELLGPPRFMPCEIYV"
     gene            10900..11226
                     /locus_tag="VF752_07800"
     CDS             10900..11226
                     /locus_tag="VF752_07800"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8855490.1"
                     /translation="MSPSAWAALADPSRRAVLELLLERPRPVGELVEKLKLSQPGTSK
                     HLRVLREAGLVWVHRDGQRRVYGIEPQPMAELDAWLAPYRRLWEDRLDALERRLDETN
                     PKEDKA"
     gene            11223..11747
                     /locus_tag="VF752_07805"
     CDS             11223..11747
                     /locus_tag="VF752_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8855491.1"
                     /translation="MTQATYQEIDGRPAIRFERRIAHPVEAVWHAVTEPGELAHWFPD
                     PVELDELKPGASMRFDLSAHGMDPMDGEIRELEPPRLFAFTWGDEMLRFELDPEDGGR
                     ACRLRFTHFLSERDKAARDAAGWQVCFDRLDESLAAGGAGAANTGVTPAWREYYERYL
                     AEGMPSGAEIPGQA"
BASE COUNT         1622 a         4005 c         4079 g         2091 t
ORIGIN      
        1 gggctgcagc atcagcggct tccctccgat ctctggggtg gcgcgccgcc accccgctga
       61 ccgccgccac gcgctccgcg gccccggccg ccacccggcc cgaccgcctg cggcgcctcg
      121 agctcggtga ggtcctgcgc gaagcccgag ggcatgatct cgcccttgtt gatccacacc
      181 ttcacgccga tgcggccgaa ggtggtacgg gcctcgtaga agccgtagtc gatgtcggcg
      241 cgcagcgtgt gcagcggcac gcggccgtcc gagtagccct cggtgcgcgc catctccgcg
      301 ccgccgaggc ggccggagac ctggatgcga acgcccttcg cgccggagcg catcgcgctg
      361 gtgagcgcac gcttcatggc acggcggaag gcgacgcggt tctgaagctg ctccgcgacc
      421 gactgcgcga cgagcttggc gtcgagctca ggccgcttga tctcgaggat gttcacgcgc
      481 accgacttgc cggtcatgcg gtgaagctcg cggcgcagcg cgtcgacctc gctgccggac
      541 ttgccgatca cgatgccggg ccgggccgtg tggatgttga cctcgacctc gttcttgtcc
      601 ttcttgatcg tgatgtcgga gagaccggcg tgcgcgagct tgccctcgat gtgattgcgg
      661 atgtggacgt cctcgagcag gtagtcggcg aactcgcgct cgttgaacca gctggacttc
      721 cagtcatgga tgtagccgac gcgaagcgac tctggatgga ctttctgacc catgcttctt
      781 cgactactcc ttcggtgaca gcgtgatcgt gaggtggctc gtgcgcttgc ggatacgcgt
      841 ggcgcggccg agagcacgag gacggtaccg cttgagcgtg ggcccttcgt cgacgtaggc
      901 ctggttgatg accaggtcgt ccgcgacgag ctcgtgattg ttctcggcgt tcgcgatggc
      961 cgagtcgaga agcttggcga catcgcgagc cgcggcacgc ggggtgtgag tgaggatagc
     1021 ccgcgcctca tcgaccgact tgccgcggat gtggtcgatc acgagtcgcg ccttccgggg
     1081 cgcggtacgc acgtagcgag cctgcgcgcg cacgaccacg acgccatcgc cgtcccgggg
     1141 cggcgcgggc ttgcgctgcg gcgtgctcgt ctccgcggcg cggcggcgag tggtacgccg
     1201 cggcttctcc tcgggctcct ccgccgcttc ctcgggttcg gccgcctcct cggtctcggc
     1261 ctgctcttcg gcggcctcca cctcggcctc cgcctccgcc tcttccttct tgcggcgggt
     1321 gcgcttcggc ttctcctcgg caggggcgga ctcggcctcc gccttcggcg cggccttctt
     1381 gcgcgagcgc gtcgcgcgct tcggcttctc ctcggcctgg gcctcggtcc tctccggcgt
     1441 ctcctcggcg ccgttctcga cggtgtcctt ctgctcctcg gtcatcgcat cctcgccttg
     1501 tcgctgccgg cgtggccgcg gaacaggcgg gtcggggcga actcgccgag cttgtggccc
     1561 accatcgact cgctcacgaa cacgggcacg tgcttgcgcc cgtcgtgcac cgcgatcgtg
     1621 tggcccacca tctcggggaa gatcgtggag gttcgggacc aggtcttcac catctgcttg
     1681 ctaccggact cgttcatggc ctcgatgcgc ctgatcagcc gctcctcgat ccacgggccc
     1741 ttcttcgaag atctcgacat ctctgcttcc tacttcctct tggcgcgcct gcggctgcgc
     1801 acgatccagc ggtcggactg cttgttcttc ttgcgcgtgg ggtagccgag cgtcggcacg
     1861 ccccacgggg ttacgggatg gcgacccggc gtggtcgagc cctcgccgcc gccgtgcggg
     1921 tggtcgacgg ggttcatggc cgtgccgcgg gtctgcgggc gcttgccgag gtgacggctg
     1981 cggcccgcct tcccgatgtc gatgttctgg tgctcggcgt tgccgatcga gcccaccgtg
     2041 gcgcggcagt cgacgtgcac catgcgcatc tcgcccgacg gcaggcggag cgtggcgcgg
     2101 tcgccctcct tcgccacgag ctgcaccgcc gcgccggcgg accggccgag ctggccgcca
     2161 cggccgggcg tgagctccac gttgtgcacg gtggtgccgg tggggatgtt gcgcagcggc
     2221 agcgcgtgcc ctggagcgat gtcggcgcca gtgcccgaca tcacggtggc gcccacgcgt
     2281 aggcggttcg gcgcgaggat gtagcgcttc tcgccgtcgg cgtagtgcag cagcgcgatg
     2341 tatgcggtgc ggttcgggtc gtactcgatc gccgcgacgc gcgctgggat gccgtccttg
     2401 cgccgcttga agtcgatcac gcggtacttg cgcttcgccc cgccgccgcg gtggcgcgag
     2461 gtgatgcggc cattcgagtt gcggccgccg ctcttgttga ggccttcgac gagcgacttc
     2521 tcgggctcgc tcttcgtgac ctccgcattg tccgcgaagg tcgcgaagcg ccggccggga
     2581 ctggtgggct tgtacttctt gatgggcatg gctttatgcg gctattcgcc gatctcctgg
     2641 ccttcgaaga gctcgatcgt gtcgtccgga tgcagctgca cgatcgcctt cttccactcg
     2701 gccgtgcgcc cgcgcgtgta gccgcggcgc ttgggcttcg acttaacgct catcgtgcgc
     2761 acgtcgagca cgcgcacgtt gaagatctcc tcgaccgcct ggcggacctg cgtcttgtgg
     2821 gcgtcgtcgt gcacctggaa cgtgtacttg ttggccgcga gtagcgcgta gctcttctcg
     2881 gacacgatcg ggcggacgat cacgctgcgg ggattcacga gtcgccgtcc tctccaccgc
     2941 tgtcgcgcgc gaccggctgg gcgagcctgg tgagctcgtc gagcgccgcc tgcgagacca
     3001 cgagccgcgt cgcgccgatg atgtcggcca cgccgaccgc gctcgcgggc agcacggcgg
     3061 cgacgtcacg caggttgcgg aacgacttcg cgcagttctc ctcgccctcg agcgcgagca
     3121 cgacgagcgt cttcccctcg ccgaatccgg ccagggcctc cgcggcttgc ccggcggacg
     3181 gcgagtcgaa cttaccggcc tccacgaccg cgagcgtgcc gcgaccagcg tgcacggaca
     3241 gggccgcgcg cagcgccttg cggcgcgctt tgcggttcac cttcaccacg tagtcgcgcg
     3301 gcttggggcc gaaggcgatg cctccgccaa ttcgctgcgg cgtggagaga gcgccggtgc
     3361 gtgcgcgacc ggtgcccttc tggcggaacg ccttcccacc ggtcatcgcg acctcgccgc
     3421 ggctgagcgt ggacgcggtg ccgcggcggc gcgcgttcag ctcggcgcgc acggtctcgt
     3481 gcacgagcgc catgtggaag ggctcgccga acaccgacgc gtcgaggttc gccttcgtgg
     3541 tcttgccgag cacggctgcc ttcggggcgg ccatcaggag tccgtcctta tctcgaccgt
     3601 ggagcccttg gcgccgggca cggagccgcg gagcaggatc aaattctcgt caggcacgac
     3661 gtcgacgacc tcgaggccct tctgcgtgac gcgcttgttg cccatctggc ctgggccgcg
     3721 gatgcccttg aacacgcgcg aaggcgttgc ggacgcgccg atcgagcctg gcgcgcgcac
     3781 attgtgcgag ccatgggtca cggggccgcg gctgaagttg tgccgcttga ccgtgccctg
     3841 gaagcccttg cccttcgaga tgccggagac cttcacggtc tggcccttct cgaagacgtc
     3901 gacgccgacc gtgtcgccca cctgcagctc gccggcctcg tcgcgaaact ccacgaggtg
     3961 gcgcactgcc ggcacgccgg ccttcttggt gtgaccggcc tcggccttcg tgaggcgctt
     4021 ttcgttcacc tcgccgaagc cgagctgcac ggcctcgtag ccgtcacgct cgtgcgtgcg
     4081 aatgcccgtg acgaagcagg gacccgcctg cacgacggtg acgcgctcga ggcggccgtc
     4141 ctcctggaag acctgcgtca tgccgagctt ctttccgagc agtgccgcca tcggtcagac
     4201 gagctggatc tgaatgtcga cgccggccgg caggtggtcg aggcgctgga gagagtcgac
     4261 cgtcttcggc gtcggctggt ggatgtcgat cagccgcttg tgcgtgcgga tctcgaagtg
     4321 ctcgcgtgag tccttgtcct tgaacgggct gcggatcacg cagtagacgt tcttctcggt
     4381 gggcaggggc acggggccgg acacgctcgc gccggtgcgc tgagccgtgt ccacgatctc
     4441 cttcgccgcc tgctcgatcg aggagtggtc gtaggccttc agcctgatgc ggatcttgtt
     4501 ctgggtgatt gctgccatct gtttcggaag tgtcctgtgg acgcaccgag ggcgggcccg
     4561 ttaccggggc ccgcccccag aagctcctat gaggaggcgt cctactcgac gatctcggtc
     4621 acgacccccg agccgacagt gcggccgccc tcgcggatag cgaagcgcag gccctgatcc
     4681 atggcgatcg gctggatcag ctcgatcgac atctgcacgt tgtcgcccgg catgaccatc
     4741 tcggtgccct ccggcagatg ggcgacgccg gtcacgtcgg tcgtgcggaa gtagaactgc
     4801 ggccggtagc catcgaagaa cggagtgtga cggccaccct cctccttctt caggcagtag
     4861 acctcagcct tgaacttggt gtgaggcgtg atcgaccccg gcttgcagag cacctggccg
     4921 cgctcgatct cctcgcgctt ggtgccacgc agcaggcagc cgacgttgtc gcccgcgcga
     4981 ccttcgtcga ggatcttgcg gaacatctcc acgccggtga cgacggtctt cgccgtctcc
     5041 ggatggatgc cgaccagctc gacctcctcg ccggtcttga tgatgccctg ctcgatacgg
     5101 ccggtggcga cggtgccgcg gccggtgatc gagaagacgt cctcgaccgg catcaggaac
     5161 ggcttgtcca ggtcgcgctc aggctccggg atgtagctgt caagtgcctc ggcgagctcg
     5221 aggatcttgc ccttctgctc ctcgtcgccc tcgagtgcct tgagcgccga gcccgtgatg
     5281 aacgggatgt cgtcgcccgg gaactcgtac tcggacagca gatcgcggac ctcgacctcc
     5341 acgagctcga gaagctcggg gtcgtcgacc atgtcggact tgttcaggaa gaccacgatg
     5401 tacggcacgc cgacctggcg ggcgagcagg atgtgctcgc gcgtctgcgg cattgggccg
     5461 tcggccgccg acacgacgag gatcgccccg tccatctggg cggcgcccgt gatcatgttc
     5521 ttcacgtagt cggcgtgacc cgggcagtcc acgtgcgcgt agtgacggtt ctccgtctcg
     5581 tactcaacgt gactcgtggc gatcgtgatg ccgcgctcct tctcctcggg cgcgttgtcg
     5641 atctccgcga agctgcgagc ctggccgccg accttctcgg cgagcacctg tgtgatagcc
     5701 gcggtcagcg tggtcttgcc atggtcgatg tgaccgatgg tgcctacgtt gacgtgcggc
     5761 ttatcgcgct cgaacttttc cttcgccact tggcgttgct cctttcagac cttggcgggc
     5821 tcgcgcttcg gcgaacccgg aaacatactc tctatctgct ctatgcgctg gctccgacgg
     5881 gctcgccggt gcggtgctcg acgatgtcct cggcgatgtt gccggggacc tcttcgtagc
     5941 ggtcgaactg catcgtgtag gtggcccgtc cctgggtgtt cgaacgtagg tccgttgcgt
     6001 agccgaacat ctccgagagc ggcacgctgg actggaccgc gagcgcgttg ccgcgccggt
     6061 cctgaccctc gacgcggccg cgccggcgcg acaggtcgcc gatcacgtca cctaggaact
     6121 cctcgggcgt gacgacctcg acggccatga tcggctcgag cagcaccggc ttggcgcgct
     6181 tcgcgccctc cttgagcgcc agcgagccgg cgatcttgaa ggcgagctcc gacgagtcgg
     6241 tgtcgtggta cttgccgtcg gtgagcgtga cgcgcacgtc caccatcggg taaccggccc
     6301 tgacgcccgt ctcgagcgcc tcctcgatcc cgtcctcaac cgcggggatg aactccgacg
     6361 ggatggagcc gcccttgatc ttgttcacga agtcgaagcc ctcgccggga gccggctcca
     6421 cgtcgatgta gacgatgccg tactggcccg agccaccggt ctggcgcacg aagcggccct
     6481 cgaccttctg cgcggtgccg cgcacggtct cgcggtaggc gacctgcggg cgcccgaccg
     6541 cggcctcgac cttgaactcg cgcttcatgc ggtcgaccag cacctcgagg tgcagctcgc
     6601 ccatgcccga gatcacggtc tgtcccgtct cctcgtcggt ccggacctgg aacgtcgggt
     6661 cctcctccgc gaggcgcgcg agcgccgccg acatcttctc ctggtcgacc ttcgtcttcg
     6721 gctcgattgc gacgtgcacg accggctcgg ggaactcgat cgtctcgagc aggaccggag
     6781 cgtccggcgc ggacagggtg tcacccgtcg acgtctgctt gaagccgacc accgcggcga
     6841 tgtcgcccgc gtagatctcc tcctgctcct cgcggtcgtt ggcgtgcatc atcaggatgc
     6901 ggccggcacg ctcggtcttg ccgttcgacg cgttcagcac gcgtgagccg gctccgagct
     6961 tgcccgagta gacgcggaag tacgtgagct tgccgacgaa cgggtcggac atgaccttga
     7021 aggcgagcgc ggagaacggg gcgtcgtcgg acgcctcgcg cgtggccgga cggccctcct
     7081 cctcgccctt gacgggctcc aagccgtcga ccggcggcac gtcgagcggg ctcggcaggt
     7141 agtcgatcac cgcatcgagc agcggctgca cgcccttgtt cttgaaggac gagccgcaca
     7201 gcaccggcgt gatcgagatg tcgagtgtcg ccttccggat cgcggccttc aggcggtcga
     7261 ccgggatcgg ctcatcggcg aggtaggtct ccatgagcgc ctcgtcgtga tcggcgatcg
     7321 cctcgatcag gtgcgtgcgc gcctcatgcg cccggtccac ctggtcggcc gggatgtcga
     7381 tctcgtccca ctcgcggccg aggtcgtcct tgtagatgat cgccctctcg gtgaccaggt
     7441 cgatgatgcc ctggaagtcg gcctccgcgc cgatcggcag ctggatcggg atcgggttcg
     7501 caccgagccg atcgatcatc gtctggacgc cctgcgcgaa gttcgcgccg atgcggtcca
     7561 tcttgttgat gtaggcgatg cgcggcacgc ggtacttgtc ggcctggcgc cagaccgtct
     7621 cggactgcgg ctcgacgccg gctaccgagt cgaacagcgc gatcgcgccg tcgaggacgc
     7681 gcagcgagcg ctcgacctcg accgtgaagt cgacgtggcc gggggtatcg atgatgttga
     7741 tgcggtagtc gcgccagaaa gcggtcgtgg cagccgacgt gatcgtgatg ccgcgctcct
     7801 gctcctgctc catccagtcc atgacggcag caccctcatg tacctcgccg agcttgtgcg
     7861 tccggccggt gtagaaaagg atgcgctcgg tcgtggtggt cttaccggca tcgatgtgcg
     7921 ccatgatgcc gatgttgcgc gtgtgatcta gtgaaacttt ccgtggcata tctctcagcg
     7981 ggtgagccgt tcgacggcag aaaaaaggcc cttccgggcc tggtcaatcc ctcacgtgcc
     8041 cgggtgaacg ctctatgtgg cgaagttctc catttcgtgg tggcgaggcc ggactcccct
     8101 cccagatgaa ccggcttcgt gagggcttcg gatcggctcc agaagcggcg agcaatgcta
     8161 gcagaggctt ttttgcgcgc ctatcgagtt ttccgctttt gcacagggct cagcccgcgg
     8221 tcctgattct tggcctggcg ggcgggttcc tgctcgcgat caccgacttt ctgaccgtag
     8281 ttcacgtgga ggtggtgacc gccagctgcc aggacctggc cacgccgagc cttgccgaca
     8341 gctgcgtgag caagggcagc gagcagcaca gctgggccct gctcgtgctc ggcctgctgg
     8401 cgctcgcgat gacctacggc gcggccgccg gccggtcgcg cccggccgcc gcggcgctgc
     8461 tgctcatcgg tgtgggcgcg cttgccgtgt tcttcgcggt cgatctcccc gacgtttcca
     8521 agaccgggca gatcgggctg aacttcgcga acgcccaggc aaaggcgggt gcgggcttct
     8581 ggaccgagct ggtcggggcc tgtctgctcg tgttcgcggg cgcggtcgga ctcacgcgcg
     8641 cgcgtagata gccgaggtgc gcgggcgggt acgctcggcg gatggctgaa cgaaccatcg
     8701 acagcgtggc gcgggacgct ttcggcttcg aggcgctgcg ccccggccag cgcgaggcga
     8761 tcgacgcggt gctcacaggt cgcgacacgc tggcggtgat gtcgaccggc tcgggcaagt
     8821 cggccatcta ccagatcgcc ggcatgctga tggagggcgc cacgctcgtg atctcgccgc
     8881 tgatcgccct gcagcgcgac caggtggagg cgatcgacga ccagctcccg ggcgaggcgg
     8941 ccggcgtgaa ctcgacggtg gcgcgctcgg agcgggagga agcgttcgag gagctcgagg
     9001 agggagagct cgagttcctg ttcctcgcgc ccgagcagct cgcgaatccc gaggtgctcg
     9061 agaacctgcg cgaggcgcgg ccctcactgc tcgtggtcga cgaggcccac tgcatcagcg
     9121 agtggggtca cgacttccgt cctgactacc tgcgcctcgg cgcgctcgtg gacgagctcg
     9181 ggcatccgac ggtgctgggc ctcaccgcca ccgcctcgcc tcccgtccgg gacgagatcg
     9241 tcgagcggct gcacatgcgc gacccggacg tgatcgtgcg cggcttcgac cgccccaaca
     9301 tccacctggc ggtcgagcgc ttccacgagg aggagcacaa gcggcgggcg ctcgtggaag
     9361 cgatcgaggc ggccgaacgt cccgggatcg tctacgcggc cacccgcgcg atcgcggagg
     9421 agctggctga ggagctcgcc gggaagggca tcgccgcctg cgcctaccac gcgggcatga
     9481 acgcgaccga gcgcgaccgc atccaggagg agttcatgga cgacgccttc gaggtgatcg
     9541 tcgcgacgac ggcgttcggc atgggcatcg acaagccgaa catccggttc gtcttccacc
     9601 acgccgtcgc cgactcggtg gacagctatt accaggagat cgggcgcgcc ggccgcgatg
     9661 gagagccggc gcaggcgaag ctcttctaca ggacggagga cctcggcctg cgacgcttct
     9721 tcgccggggc tggacaggtc gacctcgacg agatcgccca cgtggccgat gccgttcggc
     9781 ggcacggcgg gccggtcgat cctgtggagc tgaaggacga gctcgccctc tctcagacga
     9841 agctgatcac cgcggtgaac cggctcgagg aggcgggcgc cctgcacacg cggccgacgg
     9901 gtgaggttga gctcgtgccc gacgcgccac cgccccagca gttcgtagag gaagcggcca
     9961 agcgcgagca cgaccgccac gagttcgacc gctcgcgcgt ggagatgatg cgcgcgtacg
    10021 ccgagcaccg caactgccgc cgccagttca tcctgagcta cttcggcgag gacctgcccg
    10081 agccgtgcgg caactgcgac aacgacgagg ccggcctcac tcgcgagtcg aacggcgacg
    10141 agccgttccc ggtcggcacg cgcgtggtgc accggcagtg gggcggcggc ctcgtgcagc
    10201 gctacgagga cgacgaggtc gtcgtgctgt tcgactcggt gggttacaag acgctcggcg
    10261 tgccgctcgt cctcgagcgc gacctgctcg cgcccgagta gatagaagtg gctagaagtg
    10321 gcgcctgcgc tgatccccga gcgaatcgag accgagcgcc tggccctgag gcgcaacgcc
    10381 ccggccgacc tcgacgcgtt cgtcgctatc tggagggacc ccgcggtctg gagggcgctg
    10441 atgggcggcg gcgagccgga tctcgagttc gctgcggagc ggcacgcgcg ccaggtggag
    10501 cactggcagc gcgagggctt cggcctctac ctcgtggaga tcgcgggcga ggtggccggc
    10561 tggtgcgggc cgtggcatcc cgacttcatc ccctcgctga gcagtgagat cgagatcggc
    10621 tggacgctcc ggcaccggtt ctggggcttc ggattcgcga ccgaggcggc ggcgatcgcc
    10681 gcgggcatcg cgtttgagcg cttggccccg cgccggacga tcagcctgat cgcgaaatcg
    10741 aaccgccgct cgcgggaggt tgcgaagcga ctgtcgatga gcatcgccgg agaggccgag
    10801 atcccggact ccggcatcac gctggatgtc tgggagctac ttgggccacc tcgattcatg
    10861 ccttgcgaga tatatgtctg attggttatt cttttcggca tgtcgccgtc cgcatgggca
    10921 gccctcgccg acccgagccg ccgcgccgtg ctcgagctgc tgctcgagcg gccgcgccct
    10981 gtcggtgagc tcgtcgagaa gctgaagctc agccagcccg gcacctccaa gcatctgcgc
    11041 gtgctgcgcg aggccggtct ggtctgggtt caccgcgacg gccagcggcg ggtgtatggc
    11101 atcgagcccc agccgatggc cgaactcgac gcctggctcg cgccctaccg gcggctctgg
    11161 gaggaccggc tcgatgcgct cgagcgccgg ctcgacgaaa ccaaccccaa ggaggacaag
    11221 gcatgacgca ggccacctac caggagatcg acggccgccc cgccatccgc ttcgagcggc
    11281 gcatcgcaca tccggtcgag gcggtctggc acgccgtgac tgagccgggc gaactcgcgc
    11341 attggttccc ggacccggtg gagctcgacg agctcaagcc cggcgcgagc atgcgcttcg
    11401 acctctcggc gcacggcatg gacccgatgg acggtgagat ccgcgagctc gagccgccgc
    11461 gcctgttcgc gttcacgtgg ggcgacgaga tgctgcgctt cgagctcgat ccggaggacg
    11521 gcggccgggc atgccggctc cgcttcacgc atttcctgag cgagcgcgac aaggccgcga
    11581 gggacgccgc gggctggcag gtgtgcttcg accgcctcga cgagagcctg gcggccggcg
    11641 gcgccggcgc ggcgaacacc ggggtcacgc ccgcgtggcg cgagtactac gagcgctacc
    11701 tcgccgaggg catgccgtcc ggcgcggaga tcccaggcca ggcgtagctc agagaggccg
    11761 aatgacccgg cacgggttgc ccgcggcgat cacacct
//
LOCUS       DASVXS010000142         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_726237, whole genome shotgun sequence.
ACCESSION   DASVXS010000142 DASVXS010000000
VERSION     DASVXS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726237"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF752_07810"
     CDS             complement(<1..321)
                     /locus_tag="VF752_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855492.1"
                     /translation="MAKEDKGIPTGRVRRTAKVGSVVGTSGARYAGTRARNIVRSEEA
                     GAAELDKRALESAERMVDALGNLKGAAMKIGQLASFIDTEFLPPEYRELFQDKLATLR
                     SSAPP"
     gene            368..1081
                     /locus_tag="VF752_07815"
     CDS             368..1081
                     /locus_tag="VF752_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX8855493.1"
                     /translation="MADGKRPTITVEEREERGTRETRRLRRAGYVPGVVYGGTGQEEA
                     TSFKIGARDLRNALHTGAALYDLKVGNGKALPVIVKDQQVHPVRGELMHIDLLEVRLD
                     EKIHATVSVYLEGVEDAPGVKEGGVLEQVTREVNVEALPTDIPESIVVDVSGMEAAAT
                     MHLSEVTAPSGVALLDDPDETIIATITIPTEVEEPEIEEETEVVGEEAEAAAEGATGE
                     EAEAAAEGGGGEAEGGEES"
     gene            1138..1668
                     /gene="pth"
                     /locus_tag="VF752_07820"
     CDS             1138..1668
                     /gene="pth"
                     /locus_tag="VF752_07820"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX8855494.1"
                     /translation="MVGLGNPGSRYERTRHNVGFQVGERLIERWGLGKPKKKFAGLYA
                     EGRTRPGGPRVGVLLPQTFMNDSGRSAGPARGALGVPLDHIVVIHDEIDLPFGEVRSR
                     LGGGLAGHNGLKSLKGALGGGEFQRVRVGVGRPDTTDPDIVSGYVLGRFSEPAAEVTR
                     LVDDAAAETERVLEPV"
     gene            1699..2571
                     /locus_tag="VF752_07825"
     CDS             1699..2571
                     /locus_tag="VF752_07825"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8855495.1"
                     /translation="MTETRDTKPAQAAGTFRLGDATVYRLGFGAMRITGKGIWGEPPD
                     REEARRVVRRAVELGVNLIDTADSYGPEVSEEILAEALHPYPEGLVIATKGGFLRPGP
                     GNWVPDCSPEHLREACDSSLRRLRLEQIALYQLHTVDSKVPYEESIGALKELQDEGKI
                     RHIGVSNVNVDQLHTAQEIVEVVSVQNRYNVTDRRAEDVLDDCERQGIGFIPWWPLAV
                     GELARPGGALDEVAREVGGTPSQVAIAWLLRRSPVMLPIPGTGSVQHLEENLEAARIE
                     LSEPQFDRIAEAAG"
     gene            2633..>3316
                     /locus_tag="VF752_07830"
     CDS             2633..>3316
                     /locus_tag="VF752_07830"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855496.1"
                     /translation="MSLRQLLTYAREDSNIEALAGATREAPQRAFVSASLRPYLLASL
                     IDSEPGRPALVVAGDDRAARDLASDLKAFLAPRNVRFYPARGVRYESHLAPAPHLVGL
                     RIAALDALLDDSQGADAAVVVASAPALSEKLPDPELRPHGFTIERGGLLDLEETMDTL
                     IACGYERVDQVEDRGQFAVRGDILDIYPATEERAVRCELFDIEVERLTYFSTFTQRSL
                     EEAEKVEIAP"
BASE COUNT          507 a         1142 c         1200 g          467 t
ORIGIN      
        1 aggcggggcc gagctgcgca gcgtcgcgag cttgtcctgg aacagctcgc ggtattccgg
       61 cggcaggaac tcggtgtcga tgaaggacgc gagctggccg atcttcatcg ccgcaccctt
      121 gaggttgccg agcgcgtcga ccatccgctc ggcggactcg agcgcccgct tgtcgagctc
      181 ggcggcgccg gcctcctcgg agcgcactat gttgcgggcg cgcgtgccgg cgtagcgcgc
      241 accggatgtg ccgaccactg accccacctt cgccgtgcga cgaacacgcc ccgtggggat
      301 gcccttgtcc tccttcgcca tcccggaggc aggttacttg gcctccggcg ctagactggc
      361 ccgctgcatg gcagacggaa agcgacccac aatcacggtc gaggagcgcg aggagcgcgg
      421 cacccgcgag acccggcgcc tgcgtcgcgc cggctacgtg ccgggcgtcg tctacggcgg
      481 cacgggccag gaggaggcca cgagcttcaa gatcggcgcg cgtgacctgc ggaacgcgct
      541 tcacaccggc gcggccctct acgacctgaa ggtcggtaat ggcaaggctc ttccggtgat
      601 cgtcaaggac cagcaggtcc acccggtgcg cggcgagctc atgcacatcg atctcctcga
      661 ggtgcgcctc gacgagaaga tccacgccac cgtctcggtt tatctcgagg gcgtcgagga
      721 cgctccgggc gtgaaggagg gcggtgtgct cgagcaggtc acccgcgagg tgaacgtcga
      781 ggcacttccg accgacatcc ccgagtcgat cgtcgtcgac gtctccggca tggaggccgc
      841 ggcgaccatg cacctctccg aggtgacggc gccgtccggc gtcgcgctgc tcgacgaccc
      901 cgatgagacc atcatcgcca cgatcacgat cccgaccgag gtcgaggagc cggagatcga
      961 ggaggagacc gaggtcgtgg gcgaggaggc cgaggccgcc gccgagggcg ccaccggcga
     1021 ggaggccgaa gctgccgccg agggcggcgg gggcgaggcc gagggcggcg aggagtcctg
     1081 agcctgttcc gccgctcgcg tggcggcgac gaaggcggag ggcgagtcga ctggctggtg
     1141 gtggggctcg ggaacccggg ctcgcgctac gagcgcacgc gacacaacgt gggctttcag
     1201 gtgggcgagc gcctgatcga gcgctggggc ttggggaagc cgaagaagaa gttcgcgggc
     1261 ctgtacgccg aaggacgcac gcgcccgggc ggcccgcgcg tgggggtgct cctgccgcag
     1321 acgttcatga acgattccgg gcgctcggcc ggacccgcac gcggcgcgct aggcgtgccg
     1381 ctcgaccaca tcgtcgtgat ccacgacgag atcgacctgc cgttcggtga ggtcagatcc
     1441 cggctcggcg gcggcctggc gggccacaac gggctcaagt ccctgaaggg cgcgctcggc
     1501 ggtggcgagt tccagcgcgt gcgcgtgggc gtgggccggc cggatacgac cgacccggac
     1561 atcgtgtccg gatacgtgct tgggcgcttc agcgagccgg cggccgaggt gacccgcctg
     1621 gtcgacgacg ccgcggccga gacagagcgt gtgctcgaac ccgtttagcc cgcccggaag
     1681 cgcggtatcg gcgcggacat gaccgaaacg cgagacacga agcccgcgca ggcggcagga
     1741 accttccggc tcggcgacgc gaccgtctac cgcctcggct ttggcgcgat gcgcatcacc
     1801 ggcaagggca tctggggaga gccgccggac cgcgaggagg cgcgccgcgt cgtgcggcgt
     1861 gccgtcgagc tcggagtgaa cctgatcgac accgccgatt cgtacggccc cgaggtgagc
     1921 gaggagatcc tcgctgaggc cctgcatccc tatcccgagg gcctcgtgat cgccacaaag
     1981 ggcggcttcc tgcggccggg tcccggaaac tgggtgccgg actgcagtcc ggagcacctg
     2041 cgcgaggcct gtgacagctc gctgcggcgc ctgcgcctcg agcagatcgc gctctaccag
     2101 ctgcacacag tcgactccaa ggttccgtac gaggagtcga tcggcgcgct gaaggagctc
     2161 caggacgaag gcaagatccg ccacatcggc gtctccaacg tgaacgtcga tcagctccac
     2221 accgcgcagg agatcgtcga ggtcgtctcg gtccagaatc gctacaacgt caccgaccgc
     2281 agggccgagg acgtgctcga cgactgcgag cggcagggca tcggcttcat cccgtggtgg
     2341 ccgctcgccg tcggcgagct cgcacgcccc ggcggggcgc tcgacgaggt ggcgcgggag
     2401 gttggcggca cgccctcgca ggtcgcgatt gcctggctgc tcaggcgctc gccggtgatg
     2461 ctgccgatcc cgggcaccgg ctcggtccag cacctcgagg agaacctcga ggcggctcgg
     2521 atcgagctga gcgagccgca gttcgaccgc atcgcagaag cggctgggta gcccgaccta
     2581 gaatgggtgt ggagagccgc gcggcgtgtc cgggcggccg ttttccgtgc ccatgtcact
     2641 ccgccagctg ctcacatacg cccgcgagga ctcgaacatc gaggccctcg ccggcgcgac
     2701 gcgcgaggcg ccgcagcgcg ccttcgtgtc cgccagcctg cgcccgtatc tcctcgcgtc
     2761 gctgatcgac tccgagccgg gccgccccgc gctcgtggtg gccggcgacg atcgcgcggc
     2821 ccgtgacctc gcctccgacc tgaaggcctt cctcgcgccc cggaacgtgc gcttctaccc
     2881 cgcgcgcggc gtgcgctacg agtcgcacct ggcgccggcg ccgcacctcg tgggcctgcg
     2941 aatcgcggcg ctcgacgccc tgctcgacga ttcgcagggg gccgacgcgg cggtggtcgt
     3001 ggcatccgcg cccgcgctgt cggagaagct gcccgacccc gagctgcgtc cgcacggctt
     3061 cacgatcgag cgcggcggtc tgctcgacct agaggagacc atggacacgc tgatcgcgtg
     3121 tggctacgag cgcgtggacc aggtcgagga tcgtgggcag ttcgccgtgc gtggcgacat
     3181 cctcgacatc tacccggcga ccgaggagcg cgcggtgcgc tgcgagctct tcgacatcga
     3241 ggtcgagcgg ctcacctact tctccacctt cacgcagcgc tcgctcgagg aagccgagaa
     3301 ggtcgagata gcgcct
//
LOCUS       DASVXS010000143        15394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1312398, whole genome shotgun sequence.
ACCESSION   DASVXS010000143 DASVXS010000000
VERSION     DASVXS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15394
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312398"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VF752_07835"
     CDS             complement(<1..809)
                     /locus_tag="VF752_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8855497.1"
                     /translation="MPDPGLHRDEVEAALELASLQVQGYLRGLDEGQVQPTGSVNRLA
                     ALDPPFPEKGDGALAALTELGDIAQENATRSTGPRFFHFVIGGTTPAALAADWLTSAI
                     DQNAGAWPASPLSAQLEVIVCDWLRQLFRLPDSFRGVLVTGGTMANFTGLAAARSWWG
                     QQTGIPVDEDGLAGAPPVPVFTSGIVHASAVKAIGMLGIGRSSVRKLAVDATGRLDVT
                     ALEAELAALEGAPAIVIANAGDANSGAFDPIDHMADLAERYNAWLHVDGAFG"
     gene            852..1199
                     /locus_tag="VF752_07840"
     CDS             852..1199
                     /locus_tag="VF752_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855498.1"
                     /translation="MDRTIAWKSLAAQLGAVAVLGLALGLSLPHSFFEDWGWLAGPAA
                     WAVAALFTARVVHLPSLPVLAGAALAGLPGVLAVLVGVHWAGAVAGAVVFALWCGWLA
                     ARVAGTRRDYSLQ"
     gene            1407..2705
                     /locus_tag="VF752_07845"
     CDS             1407..2705
                     /locus_tag="VF752_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8855499.1"
                     /translation="MEQATTTPDSAEATAAAEAAAAPVADPTGTGFSMALTDDQKEIR
                     DWVHGFAEKVVRPAAAEWDEREETPWPVIQEAAKIGLYGFESIAQFWADETGITLPIV
                     NEELFWGDAGIGMSIMGTTLAVAGIFASGTPEQMGEWIPQCYGTEDDPKVGAFCVSEP
                     DAGSDVSSLRTRAKYDEAKDEWVLNGQKAWATNGGIANVHVVVASVDSELKSRGQAAF
                     VIPPGTKGLEMGTKVKKHGLRASHTADVFLDDCRVPGRCLLGGKDKLDERLARAREGT
                     RANTQAAMQTFEVSRPTVGAQAIGIARAAYEYALEYAKQREQFGKPIIENQAIAFTLA
                     DMKLELDAARLLVWRASWMGRTGQRFDNAEGSMSKLKAGEVAVWATERAIQILGGNGY
                     TREYPVERMHRDAKIYTIFEGTSEIQRLVISRAISGMQIR"
     gene            2705..3613
                     /locus_tag="VF752_07850"
     CDS             2705..3613
                     /locus_tag="VF752_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8855500.1"
                     /translation="MAPRVEHHELDLHGHTVTYSTAGTRGPVLLLIHGITSSNRTWDE
                     VIPLLARDHRVVAPDLLGHGQSAKPRGDYSLGAYATGVRDVMTAIGHRRFTVLGHSLG
                     GGVAMQLAYQFPDRMERLVLVSSGGLGRDVHFLLRAASLPGSEWVLPLLSAGFLRDAG
                     TAVGRALAAVGLKLGPDLEEMARGFASLADHEARAAFVHTVRSVIDPTGQRVDARDRL
                     YLSQEVPSLIVWGDRDPIIPARHGEEAHELMPGSRFELFEGAGHFPYRDEPNRFAELL
                     AEFIRDTKPARADEHGRMRELILAGR"
     gene            complement(3614..3889)
                     /locus_tag="VF752_07855"
     CDS             complement(3614..3889)
                     /locus_tag="VF752_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX8855501.1"
                     /translation="MTHAIVLISAEREALPVLGGRLADLDGVAEAYSVTGEWDFVAIL
                     RVPEQEQMAELVTNRLARLDGIARTYTMVAFEAFSQHDLDAMFSLGD"
     gene            3986..4420
                     /locus_tag="VF752_07860"
     CDS             3986..4420
                     /locus_tag="VF752_07860"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8855502.1"
                     /translation="MPHFSESVEIKRSPEDVWTLLGHPEQWLEGYLEASDRSSDYPGP
                     GTHDDHLYRTRMKEQVTARVTRSEPGATLEEDQEGKTFKRHVQYSLSPAMGGGTFLRV
                     EDDVDFKGLGKLAGPIASRDIQQRWQTSLQKLKSAAESGAST"
     gene            complement(4425..5459)
                     /locus_tag="VF752_07865"
     CDS             complement(4425..5459)
                     /locus_tag="VF752_07865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8855503.1"
                     /translation="MLRLSVLDQSPVSEGSTGADALANSIDLARLADGLGYERYWVAE
                     HHGTPMLACAAPEVLIGPIAAATSRIRVGSGGVMLPHYSPLKVAESFSVLSALYPGRI
                     DLGIGRAPGTDPMTMYALQRDRRQAAPDDFPQQLAELLGYLNDSLPEDHPFRRLSALP
                     GGDARPEVWLLGSSLQSAVWAAQLGLPYSFADFINPNGAEIAAEYRRLFKDTEANPAP
                     RVNVAVWAICADSDEEAHRLASSSRMARAMMNSGRLIPVPPIEKAVRFLESQGDSGER
                     RGGGRRAVIGSPAKVREGIEQVARDYGAEEVMVVTITYEHAARRRSYELIADAFGLET
                     ATGAAVVGAD"
     gene            5656..5856
                     /locus_tag="VF752_07870"
     CDS             5656..5856
                     /locus_tag="VF752_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855504.1"
                     /translation="MKHVAAALEGARCVASATLREVVDGRKLRFDVEVRDGERTIGVG
                     THERRVIDVSSHAEQAKVRSGA"
     gene            5853..6497
                     /locus_tag="VF752_07875"
     CDS             5853..6497
                     /locus_tag="VF752_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX8855505.1"
                     /translation="MNADPVEIRVLGCLIEKQRTTPDAYPLTLNALRLACNQSTNRDP
                     VVDYDEATIRAALDRLGRRRWTRLTSGHGSRTPKFRHLLDEALGLDRSELALLAVLML
                     RGPQTPGELKQRTERLQHFDDIAAVEAAVDGLISRELVARLARRPGQKEERYGQLIGG
                     AETTAEAATESAPEPRAVAAPAASDGLSARVERLESDVAELRAELVRLREELGA"
     gene            6499..6852
                     /locus_tag="VF752_07880"
     CDS             6499..6852
                     /locus_tag="VF752_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX8855506.1"
                     /translation="MTQEPPGDATRRTMLAAERTWLAWWRTGIASAAAAVAVGGVVAH
                     LAGGSKTPYVVLGAGYAVLAAAVFIGAALRQREVERALERGGYVGVRGFWVIALTAAG
                     LLLALGTLVVVIVRS"
     gene            complement(6971..7120)
                     /locus_tag="VF752_07885"
     CDS             complement(6971..7120)
                     /locus_tag="VF752_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855507.1"
                     /translation="MNLPIEIIYEECDIPAEMTLREYRRVRFAGRHEARTRQGFRRFL
                     TAFVN"
     gene            7240..7755
                     /locus_tag="VF752_07890"
     CDS             7240..7755
                     /locus_tag="VF752_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8855508.1"
                     /translation="MARSAETTIRVRPAAAADVPLILTLIRELAEYERLADRVVATEE
                     RLHTHLFGERPAAEALIGEVDGRPAGFALFHTSFSTFLARPGIWLEDVFVREEHRGAG
                     LGRALLAELARIALERGYGRLEWWVLDWNEPAKGFYRSIGAEAMDAWTVQRLGEEAMH
                     RLADEIAPEAG"
     gene            complement(7752..8393)
                     /locus_tag="VF752_07895"
     CDS             complement(7752..8393)
                     /locus_tag="VF752_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8855509.1"
                     /translation="MRGSESFPGPLLDIVAETVESGDLQLTIIRPRDWAELRHQDGAD
                     GLSAPYWAIAWPSGRALAEVVAARDLSGTHVLELGCGLALPSLAAAKRGAEVLATDSS
                     PDAVVFAAHNFALNEVEGEVAQADWREADPLVESGPWDLVLAADVLYLRHNVEALLRV
                     LPALLGGEGEALIADPNRAGGRDFAAAARRMFELESRPDPGREKMTLHRLTRR"
     gene            complement(8462..10696)
                     /locus_tag="VF752_07900"
     CDS             complement(8462..10696)
                     /locus_tag="VF752_07900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8855510.1"
                     /translation="MPESVRAPEQQQVAPTVRLADDPRSGLVLVFRFAFDELLNAAVK
                     RLPGRRFDWDTREWIVPARREFAEEFAELLAVFPRIQPEPEVTAWLSDVGWAGMASVR
                     DVGYGPVLAVRSITGIVPEWLEERGETDADGRIVVPLDEDVAQLAYDSDGLELDTLAD
                     HAVENILGGAPAPSTVLDLGADDEGIERFELSVGLEVHAAEAFLRLTEAHRAGPRHGH
                     FSLPDQRDTLAVPADAALLDELDGFIDGHEFVVVTERAEMWRRELRTERRRAKATVAL
                     SMADDADLDVPTLHGELRPFQRAGVRYALEQRRTFIADEQGLGKTVQALGALELDDAY
                     PAVVVCPASLKLTWQREVSRFLPHRSTAVLHGRSRSGWLSAGAGQADIVILNYEIVDG
                     HVDSLAEFEPGAAVFDESHYCKQPRAKRTKACLALSARLPEESLRLALTGTPILNRPK
                     ELVSQLRLIGRLGDFESGAGMARRFKGREALERLHWHLRSHCYVRRLKADVLPQLPPK
                     RQVPIPVELSNETEYRLAERDLIAWLQSQPLDLRTLEARVGAALRNERLVQLNHLRRL
                     AGRGKLAASLSWIDDFLQSGEPLVVFADHIEVQQALVDRFPESVHVLGDDSVVARDAA
                     VTAFQDPAGPQLIVCSLRAAGQGLTLTKASNVAFLELDWTPARLAQAEDRCHRIGQRD
                     AVTAWYLIAPDTIDETMADLLEHKRGVIGAVTDGHVAQAESLVEGVVRALRERDLRET
                     DRAA"
     gene            complement(10757..10957)
                     /locus_tag="VF752_07905"
     CDS             complement(10757..10957)
                     /locus_tag="VF752_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855511.1"
                     /translation="MLSERRCTHCGVQLPEREPGESTTDDLVTQMLAQEALPEQDAAA
                     REPRQLVYCVKCTSCGHRNWLT"
     gene            11120..11722
                     /locus_tag="VF752_07910"
     CDS             11120..11722
                     /locus_tag="VF752_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855512.1"
                     /translation="MPRFHSKSFLHTFGRVSFVFAVVAVAYAGLQARRRNSVDVQVAG
                     AGSYSPPGSQSRPASTEMPDVAPVPEPPVMEEPVMPVTELESEPVVEFEPVTELESEP
                     LVEPEPLVEPEPVAESEPVAEPEPLVEREPLVEHEPVVESEPVVEFEPAVANEMVVAE
                     EHSSWMSPESSEPEPEPEPEPRSAPSPAPWWSDDVTSRSA"
     gene            11781..13124
                     /locus_tag="VF752_07915"
     CDS             11781..13124
                     /locus_tag="VF752_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855513.1"
                     /translation="MRGLGRVVPVALLVAAACASPAEAADPDPYCTSTSYGNEPAKPA
                     KLRFGVDPELAGSVGTGQSTAKPLDNAKDVAALHALTPTGRDMVLRINRLFWADGEAG
                     IKRFQAIVARNSAEGLDSELQVRYHPATGDEGDIAKWTRYVRHVVDVFGTDRHVVAMT
                     ITNEVNLNVSQNTSDGAYAGATDALIQGVIAARDEADRIGRPDMKFGFTFAFRWNPIS
                     DASFWNKLGAAPAAFRRAIGFVGVDDYPGTFWPPVIAPTSSPGRELAIALATVRRCYT
                     PKAGLGSSVPIWVTENGYQSGAAGDDAAQVVALEDMVRTAASVAGTYGVTDYRWFNLR
                     DNLSRSPGIFDTTGLLHDDYSKKPAFGRYRALVAEFGSNKTPASGPRACRAAVRLTLP
                     RRARSYRVRVDGRRVRARRHGRRLLVRARPGARVTLVARLQGGRRTFVHKRARTC"
     gene            complement(13191..14513)
                     /locus_tag="VF752_07920"
     CDS             complement(13191..14513)
                     /locus_tag="VF752_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8855514.1"
                     /translation="MSARAASTGLEGQLPPGPRLPYALQTLLVWQFTGPFLRYCRRRY
                     GRTFTVRAFPAKASVYVTDPEDLKAAFAADPDVLRAGEANAILGPVLGRRSVLLSDGD
                     EHLRRRRLMLPFFHGDSVRRYTEVVEDVADAEIATWPAGKPFRLHDRMQAITLEVILR
                     AVIGVDAPERLSELRVALRRTVEFAPGVMLMWVWPRLGAVGRWRRQRRWQTEAETLLH
                     DEIARRRAVPDLEERTDVLSLLIRARYDDGSAMDDDELRDQVMTLLLAGHETTATGLA
                     WAFERLARNPEVMTKAQRAADDGDDDYLDALVKETLRTRPVIADVARVAVEPIRLAGY
                     DLPAGVTVMPVISLVQQGDAFTDAGAFMPERFLEGRGSTPYTWIPFGGGRRRCLGAAF
                     ASLEMRLVIKRVLQRVELRAASARPEWAVPRHITLVPGRGTRLSVRPR"
     gene            14637..15233
                     /locus_tag="VF752_07925"
     CDS             14637..15233
                     /locus_tag="VF752_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8855515.1"
                     /translation="MSNRDALLEGAKRCLREKGYANTTARDLVAASGTNLSSIGYHFG
                     SKEALLAEAFDEAFAEWTAQLNAVALDSPAATALDRVAASWKAMLDMLPEHERLMLAF
                     VESIGPSVRSPELRARLAEHYERTRTDVSDVVKRTLGEDALEAGADPDVVASYLIAVA
                     DGFMIQFLIDPSRVPSGDQLVAALGAALASAVAASTPA"
BASE COUNT         2332 a         5265 c         5523 g         2274 t
ORIGIN      
        1 ccgaacgcgc cgtcgacatg cagccaggcg ttgtagcgct cggcgaggtc ggccatgtgg
       61 tcgatcggat cgaacgcgcc ggagttggcg tcgccggcgt tcgcgatcac gatcgcgggc
      121 gcgccttcga gcgcggccag ctcggcttcg agcgcggtca cgtcgaggcg gccggtggcg
      181 tcaaccgcga gctttcgcac gctggagcgg ccgattccga gcatgccgat cgccttgacg
      241 gcgctcgcgt gcacgatgcc gctggtgaac accgggaccg gtggagcgcc cgcgagaccg
      301 tcctcgtcca ccgggatgcc ggtctgctgc ccccaccagc tgcgcgcggc cgcgagaccg
      361 gtgaagttgg ccatggtgcc gccggtcacg agcacgcccc ggaagctgtc gggcaggcgg
      421 aagagctgac gcagccagtc gcagacgatc acctcgagct gcgcgctcaa tggtgaggca
      481 ggccatgcgc ctgcgttctg gtcgatcgcc gacgtcagcc agtccgccgc taatgccgcg
      541 ggcgtcgtgc cgccgatcac gaagtggaag aaacgcggcc cggtcgagcg cgtggcgttc
      601 tcctgcgcta tatcgccgag ctcggtcagc gccgcgagag cgccgtcgcc cttctccggg
      661 aagggcggat ccagcgcggc aagcctgttg acggacccgg tcggctgcac ctgcccttcg
      721 tcgagtcctc gcaggtagcc ctgcacctgg agcgaagcga gctcgagggc ggcctcgacc
      781 tcgtcgcggt gcaggccggg gtcgggcatg gcgcgatgct acgccgaacg ccgcgctgcc
      841 acaatcgacg cgtggatcgc acgatcgcat ggaagtcgct cgcggctcag ctcggcgccg
      901 ttgcagtgct cgggctggcg ctaggcctgt cgctgccgca ctcgttcttc gaggactggg
      961 gctggctggc gggcccggcg gcgtgggcgg tcgcggcgct gttcaccgcc cgggtggtgc
     1021 acctgccgtc gctgccggtg ctcgcgggcg ccgcgctcgc cgggctgccg ggcgtgctcg
     1081 ccgtcctcgt cggcgtgcac tgggcgggcg ccgtggcggg agcggtcgtc tttgcgctgt
     1141 ggtgcggctg gctggcggct cgcgttgccg gcacccggcg cgattacagc ttgcagtaag
     1201 gggaggccgc attcgtcggg gacctcccgg cagctgggcg ctaacggccg tttgcggcct
     1261 ccacgctccc gtaagtgctg cttttcgcgc ctggcgagcg cggcgtagcg aatccacgcg
     1321 gccgcgcaca cgcgtgcccg cacgcggtaa cctgggtaca tcaactgaac gattggtcag
     1381 tagacgtacc ccaggaggtc gagacgatgg agcaggcgac gacaacgccg gacagcgccg
     1441 aagccacagc ggcggcggag gcagcggcag caccggtcgc cgacccgacg ggcacgggct
     1501 tctccatggc cctgaccgac gaccagaagg agattcgcga ctgggtccac ggcttcgccg
     1561 agaaggtcgt gcggccagcc gcagccgagt gggacgagcg agaggagacg ccctggcccg
     1621 tgatccagga ggccgcgaag atcgggctct acggcttcga gtcgatcgct cagttctggg
     1681 ccgacgagac cggcatcacg ctcccgatcg tgaacgagga gctgttctgg ggtgacgccg
     1741 gcattggcat gtcgatcatg ggcacgacgc tcgccgtcgc gggcatcttt gcgtcgggca
     1801 cgcccgagca gatgggggag tggatcccgc agtgctacgg caccgaggac gatccgaagg
     1861 tcggcgcctt ctgcgtgtcc gagccggacg ccggctcgga cgtgtcttcg ctccgcacgc
     1921 gcgccaagta cgacgaggcc aaggacgagt gggtcctgaa cggccagaag gcctgggcca
     1981 ccaacggcgg catcgccaac gtgcacgtgg tggtcgcctc agtggactct gagctgaagt
     2041 cgcgtggcca ggcggcgttc gtgatcccgc ccggaaccaa gggcctcgag atgggcacga
     2101 aggtgaagaa gcacggcctg cgcgcctcgc acactgccga cgtgttcctc gacgactgcc
     2161 gcgttcccgg gcgctgcctg ctcggcggca aggacaagct cgacgagcgc ctcgctcgcg
     2221 cgcgcgaggg cacccgcgcg aacacccaag ccgcgatgca gacgttcgag gtgagccgcc
     2281 cgacggtcgg cgcacaggcg atcggcatcg cgcgcgccgc gtacgagtac gcgctcgagt
     2341 acgcgaagca gcgcgagcag ttcggcaagc cgatcatcga gaaccaggcg atcgcgttca
     2401 cgctggccga catgaagctc gagctcgacg cggcgcgcct gctcgtgtgg cgcgcctcct
     2461 ggatgggacg cacgggccag cgcttcgaca acgccgaggg gtcgatgtcg aagctgaagg
     2521 ctggtgaggt cgccgtgtgg gccaccgagc gggcgatcca gatcctcggc gggaacggtt
     2581 acacgcgcga atacccggtt gagcgcatgc accgcgacgc caagatctac acgatcttcg
     2641 agggcacctc ggagatccag cgcctcgtga tctcgcgcgc gatctcgggc atgcagatcc
     2701 gctgatggcg ccgcgggtag agcatcacga gctcgacctc cacgggcaca cggtcacata
     2761 ctcgacggcc ggcacccgcg gccccgtgct tctgctgatc cacggcatca cgagcagcaa
     2821 ccgcacctgg gacgaagtga tcccgctgct cgcgcgcgat caccgcgtgg tggcgcccga
     2881 cctgctcggc cacggtcagt ccgcgaagcc gcgcggcgac tactcgctcg gcgcctacgc
     2941 caccggcgtg cgcgacgtga tgaccgccat cggccaccgg cgcttcacgg tgctcggcca
     3001 ttcgctgggc ggaggcgtgg cgatgcagct cgcctaccag ttccccgatc gcatggagcg
     3061 actcgtgctg gtgtcgagcg gcgggctcgg ccgtgacgtg cacttcctgc tgcgcgccgc
     3121 ctcgctgccg ggctccgagt gggtgctgcc gctgctcagc gccggcttcc tacgcgacgc
     3181 cggcactgcc gtcggccgcg cgcttgccgc ggtcgggctg aagctcggcc ccgatctgga
     3241 ggagatggcg cgcgggttcg cctcgctcgc cgaccatgag gcgcgagccg cgttcgtgca
     3301 cacggtgcgc tcggtgatcg atccgacggg gcagcgcgtg gacgcccgcg accgcctcta
     3361 cctatcccag gaggtgccgt cgctgatcgt ctggggagat cgcgacccga tcatcccggc
     3421 acgtcacggc gaggaggcgc acgagctgat gccgggcagc cgcttcgagc tgttcgaggg
     3481 agccggtcac ttcccgtacc gcgacgagcc gaaccgcttc gcggagctgc tcgccgagtt
     3541 catccgcgac acgaagcccg cacgcgcaga cgagcacggg cgcatgcgcg agctcatcct
     3601 cgccggccgg tagctagtcc ccgagcgaga acatcgcgtc gaggtcgtgc tgcgagaagg
     3661 cctcgaaggc gaccatcgtg taggtgcgtg cgatgccgtc gagtcgcgcg agccggttcg
     3721 tgaccagctc ggccatctgc tcctgctccg gcacccgcag gatggcgacg aagtcccatt
     3781 cgcccgtgac cgagtacgcc tccgctacgc cgtcgaggtc ggcaagtcgg ccgccgagca
     3841 ccggcagcgc ctcgcgctcc gcgcttatca gcacgatggc gtgcgtcacc ggcggcatcc
     3901 tacgcgctga ccaaacgcag ggcgcggctt gcgccctcca tgggggcggc gtagtgtcgg
     3961 ttcgaagaga gtcgaagggg gttcgatgcc gcacttcagc gagagcgtcg agatcaagcg
     4021 ctcacccgag gacgtctgga ccctgctcgg ccatcccgag caatggctcg agggctatct
     4081 cgaggccagc gaccgttcgt ccgactaccc cggcccgggc acgcacgacg atcacctcta
     4141 caggacccgc atgaaggagc aggtgacggc gagggtcacg cgctccgagc cgggcgccac
     4201 gctcgaggag gatcaggagg ggaagacgtt caagcgacac gtgcagtaca gccttagccc
     4261 cgcaatgggg ggcggcacct tcctgcgcgt ggaagacgac gtggacttca aggggctcgg
     4321 gaagctcgcc gggccgatcg cttcgcgcga catccagcag cgctggcaga cgtcgctgca
     4381 gaagctgaag tcggcggccg agtctggcgc gtcgacctga ggcttcagtc ggcgccgacg
     4441 acggcagcgc cagttgcggt ctcgaggccg aacgcgtcgg cgatcagctc atacgagcgc
     4501 cgccgcgccg cgtgctcgta ggtgatggtc acgaccatca cctcctcagc cccgtagtcg
     4561 cgcgctacct gctcgatccc ctcgcgcacc ttcgccgggg agccgatcac ggcgcgtcgg
     4621 ccgccgccgc ggcgctcacc actgtcgccc tgcgactcga ggaaccggac cgccttctcg
     4681 atcgggggga ccgggatcag tcgcccggag ttcatcatcg cccgcgccat gcggctgctc
     4741 gatgcgagcc ggtgcgcctc ctcgtcgctg tcggcgcaga tcgcccagac cgccacattc
     4801 acgcgcgggg ccgggttcgc ctcggtgtcc ttgaacagcc gcctgtactc cgcggcgatt
     4861 tcggcgccgt tggggttgat gaagtcggcg aaggagtacg gcagcccgag ctgggctgcc
     4921 cacactgcgc tctgcagcga cgagccgagc agccacacct ccggtcgggc gtccccgccg
     4981 ggcagcgccg acagtcgccg gaatgggtgg tcttcgggaa ggctgtcgtt caggtagccg
     5041 agcagctcgg caagctgctg cgggaagtcg tctggagccg cttggcggcg gtcgcgctgg
     5101 agcgcgtaca tcgtcatagg gtcggtgccg ggcgcgcggc cgatgccgag gtcgatccgg
     5161 cctggataga gcgcgctcag cacgctgaag ctctccgcca ccttcagcgg gctgtagtgc
     5221 ggcagcatca cgccgccgct gcccacgcgg atgcgactcg tggccgcggc gatcgggccg
     5281 atcagcacct cgggcgccgc gcaggccagc atcggcgtgc cgtggtgctc cgcgacccag
     5341 taacgctcgt agccgagccc gtcggcgagg cgggcgaggt cgatcgagtt tgcgagcgcg
     5401 tccgcgccgg tggacccctc ggacaccggc gactggtcga gaacactcag gcgcagcacg
     5461 ttcgacagcg tactcagcgg gtcgcttcgg caaataggat cggcccgtgc cgctcgagcc
     5521 aggcgtagcc cgcaaagaac gagttcaccg tcgaaggggg cctgctcacc gacgtccgcg
     5581 gcacccttcg gcgcgagcag cctggccgcc gaccaccttg ccgagggcca gggcaccgtt
     5641 ggcttcgagg tatgcgtgaa gcacgtggcg gctgcgctcg agggcgcgcg ctgcgtggca
     5701 agcgcgaccc ttcgcgaggt cgtcgacggg cgcaagcttc gcttcgacgt ggaggtgcgc
     5761 gacggcgagc gcacgatcgg cgtcggcacg cacgagcgcc gggtgattga cgtctcgtct
     5821 cacgcggagc aggcgaaggt acgctcaggc gcgtgaacgc cgacccggtc gagatccgcg
     5881 tgctcggctg cctcatcgag aagcagcgga ccacgcccga cgcctatccg ctgacactga
     5941 acgcgctgag gctggcgtgt aaccagtcga ccaaccgcga tccggtcgtc gactacgacg
     6001 aggccaccat ccgggccgcg ctcgaccggc tgggacgacg ccgctggacg cggctcacga
     6061 gcggtcacgg cagccgcacc ccgaagttcc ggcacctgct cgacgaggcg ctcgggctgg
     6121 accggtcgga gctggcgctg ctcgccgtgc tgatgctgcg cgggccgcag acgccgggcg
     6181 agctcaagca gcgcaccgag cgcctgcagc acttcgacga catcgccgcc gtcgaggcgg
     6241 cggtcgacgg gctgatctct cgggagctgg tcgcgcggct cgcccgccgg ccggggcaga
     6301 aggaggagcg ctacgggcag ctgatcggcg gcgccgagac gaccgccgag gcggcgactg
     6361 agagcgcacc cgagcctcgc gccgtggccg cgcccgccgc gtcggacggc ctgagcgcgc
     6421 gcgtcgagcg actcgagtcc gacgttgcgg agctccgcgc cgagctggtg cggctgcgcg
     6481 aggagctggg agcctgatgt gacgcaggag cctccgggag acgcaactcg gcgaacgatg
     6541 ctcgccgccg agcggacctg gctcgcctgg tggcgcacag gcatcgcgag cgcggccgcg
     6601 gcggtcgcgg tcggcggcgt cgtcgcgcac ctcgcgggtg gatcgaagac cccgtacgtc
     6661 gtgctcggcg cgggctacgc ggtcttggcg gcggccgtct tcatcggcgc cgcgctcaga
     6721 cagcgcgagg tggagcgcgc gctggagcgc ggcggctatg tgggggtgcg cggtttttgg
     6781 gtgatcgcgc tcacggctgc tggcctgctg cttgctctcg gaaccctggt cgtcgtgatc
     6841 gtgcgctcct gagcggcgtg gcttctgacg ggcttctcaa acccgagcgg acgctggtgc
     6901 cgggagtgcg ctatcaccgc gccatggccg aggcctccga ggggtacctc gccgaggtcg
     6961 aagtccgcgc ctagttgacg aaagcggtaa ggaagcgacg gaaaccctga cgagtgcgcg
     7021 cctcgtggcg tccggcgaac cgcacgcggc ggtactcgcg cagcgtcatc tcggcgggga
     7081 tgtcgcactc ctcgtagatg atctcgattg gaaggttcac gccaatcact cctatgcata
     7141 gtctgcgctc gcgggcggac gtaacacagg tggcacgcac ccttcttcag gttggagaac
     7201 ccgaacccga aggggggtgt ccctaccctg cacgtcggga tggcgagatc cgctgagacg
     7261 acaatccggg ttcgcccggc cgcggcggcg gacgtgccgc tgatcctgac gctcattcgc
     7321 gagcttgcgg agtacgagcg gctggcggat cgggtggtgg cgaccgagga gcggctgcac
     7381 acgcacctgt tcggcgagcg cccggccgcc gaggcgctga taggcgaggt ggacggccgg
     7441 cccgcgggct tcgcgctgtt ccacacgagc ttctcgacgt tcctggctcg gcccggcatt
     7501 tggctcgagg acgtgttcgt gcgcgaggag caccgcgggg ccgggctggg tcgggcgctg
     7561 ctggcggagc tggccaggat cgcgctcgag cgcggctatg gccggctcga gtggtgggtg
     7621 ctcgactgga acgagcccgc aaagggcttt taccgctcga tcggcgctga ggcgatggat
     7681 gcctggacgg tgcagcgcct gggcgaagag gcgatgcacc gcctcgcgga cgaaatcgct
     7741 cccgaggcgg gttagcgccg cgtcaggcgg tgcagcgtca tcttctcgcg cccgggatcg
     7801 ggccgactct cgagctcgaa cattctgcgt gcggccgctg cgaagtcgcg gccgcccgcg
     7861 cggttcgggt cggcgatcag cgcctcgcct tctccgccga gcagcgcggg caggacccgc
     7921 agcagcgcct ccacgttgtg gcggaggtag agcacgtcgg cggcgagcac gagatcccac
     7981 ggaccgctct ccacgagcgg gtcggcctcg cgccagtcgg cctgcgcgac ctcgccctcg
     8041 acctcgttca gggcaaagtt gtgggcggcg aagaccacgg cgtcggggga gctgtcggtg
     8101 gcgagcacct cagcaccgcg cttggcggcg gccaaggagg ggagggcgag gccgcaaccg
     8161 agctcgagca catgcgtgcc gctcaggtcc cgggccgcga ccacctcggc gagcgcccgc
     8221 ccgctcggcc aggcgatcgc ccagtacgga gccgagaggc cgtcggcgcc gtcctgatgc
     8281 cgcagctcgg cccagtcgcg cgggcggatg atcgtcagct gcaggtcacc gctctcgacc
     8341 gtttcagcga cgatgtcgag cagcggcccg gggaacgact cgctgccccg catctgggcc
     8401 aggtgctcgg cgagcgacgc gcgtaggggg tcgggggagg tcgacgtcgt actgacgcag
     8461 gttacgccgc gcgatcggtc tcccgaagat cccgttcgcg cagcgctcgg accacgccct
     8521 cgacgagcga ctcggcctgc gccacgtgac cgtcggtgac cgcgccgatc acgccgcgct
     8581 tgtgctcgag caggtcggcc atcgtttcgt cgatcgtgtc cggcgcgatc aggtaccagg
     8641 cggtgacggc gtcgcgctgg ccgatccggt ggcagcggtc ctcagcctga gcaagccgcg
     8701 ccggcgtcca gtcgagctcg aggaaggcca cgttcgaggc cttggtgagc gtgagcccct
     8761 gacccgcggc gcggagcgag cagacgatga gctgggggcc ggccgggtcc tggaaggcgg
     8821 tgacggcggc gtcgcgcgcg accacgctgt cgtcaccgag cacgtggacc gactcgggga
     8881 agcgatcgac gagcgcctgc tgcacctcga tgtggtcggc gaacacgacg agcggctcgc
     8941 cggactggag gaagtcgtcg atccaagaga gggaggcggc cagcttgccg cgtccggcga
     9001 gccggcgcag atggttcagc tgcaccaggc gctcgttgcg aagcgctgcg cccacccttg
     9061 cctcgagcgt gcgcaggtcg agcggctgcg actggagcca ggcgatcagg tcgcgctccg
     9121 cgagccggta ctcggtctcg ttcgagagct cgaccgggat gggcacctgg cgcttcggcg
     9181 gcagttgcgg cagcacgtct gccttgaggc ggcgcacgta gcagtgcgag cgcaggtgcc
     9241 agtggaggcg ctcgagggcc tcccggccct tgaagcgacg cgccatcccg gcgccggact
     9301 cgaagtcgcc gaggcggccg atcaggcgga gctgcgagac gagctctttc gggcggttga
     9361 ggatcggcgt gcccgtaagc gcgagccgca ggctctcctc gggcaacctt gccgagagcg
     9421 cgaggcaggc cttcgtgcgc ttggcgcgcg gctgcttgca gtagtgcgac tcgtcgaaca
     9481 ccgccgcgcc cggctcgaac tcggcgaggc tgtccacgtg gccgtcgacg atctcgtagt
     9541 tgagaatcac gatgtccgcc tggccggcgc cggcgctgag ccacccgctg cgcgagcgtc
     9601 cgtgcagcac cgcggtgctt cggtggggaa ggaagcgcga cacctcgcgc tgccaggtga
     9661 gcttcagcga ggcggggcag accaccaccg ccggatacgc gtcgtccagc tcgagggctc
     9721 ccagcgcctg cacggtcttg ccgagcccct gctcgtcggc gatgaaggtg cgtcgctgtt
     9781 ccagcgcgta acgcaccccg gcgcgctgga agggccgcag ctcgccgtgc agggtcggca
     9841 cgtcgaggtc ggcgtcgtcg gccatcgaga gggcgaccgt tgccttggcg cgacggcgct
     9901 cggtgcggag ctcgcgccgc cacatctccg cccgctcggt aacgaccacg aactcgtggc
     9961 cgtcgatgaa gccgtcgagc tcgtcgagga gcgccgcatc ggccgggacg gcgagcgtgt
    10021 cgcgctggtc ggggagcgag aaatgcccgt gccgcggccc ggcgcgatgt gcctcggtga
    10081 gccgcaggaa agcctcggcg gcgtggacct cgaggcccac gctcaactcg aagcgctcga
    10141 tgccctcgtc atcggcgccg aggtcgagca cggtactcgg cgccggcgca ccgccgagga
    10201 tgttctcgac cgcgtgatcg gcgagcgtgt ccagctccag gccgtcggag tcgtacgcga
    10261 gctgggcgac gtcctcgtcg agcggcacga cgatgcggcc gtcggcgtcg gtctcgccgc
    10321 gttcctcgag ccactcgggc acgatccccg tgatcgagcg aacggcgagc accgggccgt
    10381 agccgacgtc gcgaacgctg gccatgcctg cccagcccac gtcggaaagc caggcggtga
    10441 cctcgggctc gggctggatc cgcgggaata cggcgagcag ctcggcgaac tcctccgcga
    10501 actcgcggcg cgccggcacg atccactcgc gcgtgtccca gtcgaagcgg cgaccgggga
    10561 ggcgcttgac cgcagcgttc agcagctcgt cgaaggcgaa gcggaagacc aaaacgagtc
    10621 cgctgcgcgg gtcgtcggcg aggcggacgg tcggcgcgac ctgctgctgt tccggggcac
    10681 ggactgactc gggcataacg gtcgaatcta ggagacgcgt cggacttcgc ccagtccggc
    10741 gtcaggggag acgccgttat gtcaaccagt tgcgatgacc gcagctcgtg cacttgacgc
    10801 agtacacaag ctgcctcggc tcgcgagccg cagcgtcctg ctccgggagc gcctcctggg
    10861 cgagcatctg cgtcacgagg tcatccgtcg tggactcgcc gggctcgcgt tccgggagct
    10921 ggacgccgca gtgggtgcat cgccgctcgg agagcacggc tcaatgatgc tccggtgcgc
    10981 ggtttcgcgc caccgggccg gagcgcgggt cgcctagcga tttggaccac gcggtggcga
    11041 ggccagtcaa gtagcgccga gatgtcgccg acaaccatcg agcggggggc gtcggcaatc
    11101 gacctccaag ggagatcaca tgcctcgctt ccactccaaa agcttcctgc acacgttcgg
    11161 cagggtgtcg ttcgtgttcg ccgttgtcgc ggttgcctat gcgggcctcc aggcccgccg
    11221 ccgcaactcc gttgacgtcc aggtggccgg cgcgggctcg tactcgccgc ccggctctca
    11281 atcgcggcct gcctcgaccg aaatgcctga tgtcgccccg gtgcccgagc cgccagtgat
    11341 ggaggagccc gtcatgccgg tgactgagct cgagtccgag ccggtcgtcg agttcgagcc
    11401 ggtgactgag ctcgagtccg agccgctggt cgagcccgag ccgctggtcg agcccgagcc
    11461 cgtcgccgag tccgagccgg tggccgagcc tgagccgctg gtcgagcgcg agccgctcgt
    11521 cgagcacgag ccggtcgtgg agtccgagcc ggtcgtggag ttcgagcctg cggtggccaa
    11581 cgagatggtg gttgccgagg agcactcgag ctggatgagc ccggagtcgt ccgagccaga
    11641 gccggagccc gagccggagc ctcggtcggc tccctcaccg gcgccgtggt ggagcgacga
    11701 cgtgacatcg cgctcggcct gagccggcgc ggcctgcgcg ccgccgtcta gcgaacgaag
    11761 ctctgggaga atgcaccccg atgcgcgggc tgggcagggt cgttccggta gctctcctcg
    11821 tggcggcggc gtgcgcctca ccggccgagg cggctgatcc agacccgtac tgcacgtcga
    11881 cgagctacgg gaacgagcct gcgaagccgg cgaagctgcg gttcggagtc gatccggagc
    11941 tggcgggcag tgtcggcaca ggccagtcga cggcgaagcc gctcgacaac gcaaaggacg
    12001 ttgcggcgct gcatgcgctc acgccgaccg gccgggacat ggttctgcgg atcaaccgcc
    12061 tgttctgggc cgacggggag gcgggaatca agcgattcca ggcgatcgtc gcccgcaaca
    12121 gcgccgaggg cctcgacagc gagctgcagg tgcgttacca cccggcgacg ggcgacgagg
    12181 gcgacattgc caagtggact cgctacgtcc gtcatgtcgt ggacgtcttc ggcacggacc
    12241 ggcacgtcgt cgcgatgacg atcaccaacg aggtgaacct gaatgtctcg cagaacacga
    12301 gcgacggtgc gtacgccggc gccacggacg cgctcatcca gggcgtgatc gccgcgcgcg
    12361 acgaggccga ccggattggc cgacccgaca tgaaattcgg gttcacgttc gcgttccgct
    12421 ggaaccccat ctcggacgca agcttctgga acaagctcgg tgccgcgccg gcagcgttcc
    12481 gccgagcgat cgggttcgtg ggggtcgacg actacccggg cacgttctgg ccgccggtga
    12541 tcgcacccac gagctcgccc gggcgagagc ttgcaatagc tcttgcaacc gtgcggcgct
    12601 gctacacgcc gaaggcggga ctcggctcga gcgtgccgat ctgggtgacc gagaacggct
    12661 accagtcggg cgccgccggc gacgacgccg cacaggtcgt cgcgctcgaa gacatggtcc
    12721 ggacggcggc gtcagttgcg ggcacctacg gcgttaccga ctaccgctgg ttcaacctcc
    12781 gcgacaacct gagccgctcg ccgggcatct tcgacacgac gggcctgttg cacgacgact
    12841 actcgaagaa gccggcgttc gggcgttatc gggccctcgt cgcggagttc gggtcgaaca
    12901 agacgcccgc gagcggtccc agggcgtgcc gcgcggctgt tcggctgacc ctgccacggc
    12961 gcgcgcgctc ataccgcgtc cgcgtcgacg ggcggcgcgt gcgtgcgcgc cgtcacggcc
    13021 ggcgactgct cgtgcgggct cgtccgggcg cgcgagtgac cctcgttgcc cggctgcagg
    13081 gaggacggag gaccttcgtc cacaagcgcg cgcggacctg ctgacccgca ttttcaacgt
    13141 cggtatccat aacctccgtt atcgagcatt gggcgcgggg ctcagacaac tcaccgtggc
    13201 cggacgctaa gtctcgttcc gcgcccggga acgagcgtga tgtgccgcgg gaccgcccat
    13261 tcgggtcggg cgctggccgc gcggagctcg acccgttgga gcacgcgctt gatgacgagg
    13321 cgcatctcga ggctggcgaa ggcggcacct agacagcgcc gtcggccgcc tccgaaggga
    13381 atccaggtgt acggagtaga tccacgtccc tcgaggaacc gctcgggcat gaaggcgcca
    13441 gcgtccgtga atgcgtcgcc ctgctgcacc agactgatca ccggcatgac cgtgactccg
    13501 gccggcaggt catatccggc gagcctgatc ggctcgaccg ccacacgggc tacgtcggcg
    13561 atcaccggac gagtccggag cgtctccttg acgagcgcgt ccaggtaatc gtcgtcgccg
    13621 tcgtcagccg cgcgttgcgc tttggtcatg acctcggggt tgcgagcgag gcgctcaaac
    13681 gcccacgcga gtccggtggc agtcgtctcg tggccggcga gcagcagtgt catcacctga
    13741 tcgcggagct cgtcgtcgtc catggcggag ccgtcgtcgt agcgcgcgcg gatcaacagc
    13801 gacagcacgt cggtgcgttc ctcgagatcc ggcacggcgc gccggcgcgc gatctcgtcg
    13861 tgcaacagcg tctcagcctc cgtttgccag cgccgctggc gacgccaacg acccaccgcg
    13921 ccgagacggg gccacaccca catcagcatc acgccgggag caaactccac ggtccggcgc
    13981 agcgccaccc gcagctccga cagccgctcc ggagcgtcca cgccgatcac ggcacgcagg
    14041 atcacttcga gcgtgattgc ctgcatcctg tcgtgaagac ggaacggctt gcccgccggc
    14101 caggtcgcga tctccgcgtc ggcgacgtct tccaccacct cggtataacg acgcacactg
    14161 tcgccatgaa agaagggcag catcagccgc cgccggcgca ggtgctcgtc gccgtcgctg
    14221 agaagaacgg agcgccggcc gaggactggt ccaaggatcg cgtttgcctc cccggcacgc
    14281 agcacatcgg ggtccgccgc gaacgccgcc ttcagatcct cggggtcagt gacgtacacc
    14341 gacgcctttg ccggaaaggc ccgcaccgtg aaggtacgcc cgtaccgccg ccggcaatag
    14401 cgcaggaacg gccccgtgaa ctgccagacg agcaaggttt gcagggcata tggcagtcgt
    14461 ggccccggcg gcagttgccc ctcaagtccg gtactcgcgg ctctggctga catctcgacg
    14521 gccaagactg cctcccctgc tcggcgaacc gagttggcac attacagagc aataggacgg
    14581 atgtctagga cgattgtcct agacggtcgt ccacccgcgg ccctacactc gcggccatga
    14641 gcaatcgcga cgcgttgctt gagggcgcga agcgctgcct acgcgaaaag ggatacgcga
    14701 acacgaccgc ccgtgacctc gtcgccgcgt ccggaacgaa cctctcctcg atcggttacc
    14761 acttcggctc gaaggaggcc ctgctcgccg aggcgttcga tgaagcgttc gcggagtgga
    14821 cggcgcagct gaacgcggtc gcactggaca gcccggcggc caccgccctc gatcgggttg
    14881 ccgcgtcctg gaaggcgatg ctcgacatgc ttccggagca tgagcggctc atgctcgcct
    14941 tcgtcgagtc gatcggccca agcgtgaggt ctcccgagct gcgcgcccgg ctcgcggagc
    15001 attacgagcg gacccggacc gacgtctcgg atgtcgtcaa gcgcaccctc ggcgaagatg
    15061 ctctcgaggc aggcgcggat cctgatgtcg ttgcgtcgta tctcatcgcg gtggccgacg
    15121 ggttcatgat ccagttcctg atcgacccga gccgggttcc cagcggtgat cagctcgtcg
    15181 ccgctctcgg cgctgcgctc gcctcggcgg tcgccgcgtc aacgcccgcg tgagcggcga
    15241 cgtccggctc ccccactcgc cgcgcgggac actgctgcgc gcggccggag tcgcgctcac
    15301 cggctcaggt acgatggcac gacgcacgcc acgatcacgc gtcgaggtca tgcatgagat
    15361 ctacgtgagc gttgtcagtc ccgaggaagc ctcg
//
LOCUS       DASVXS010000144        11244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_394140, whole genome shotgun sequence.
ACCESSION   DASVXS010000144 DASVXS010000000
VERSION     DASVXS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394140"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF752_07930"
     CDS             <1..279
                     /locus_tag="VF752_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855516.1"
                     /translation="KSLDSATQKARSGEAPTLSVAGRTVEPGSGGVFKTAAAANGVPG
                     PMLAALIGIAVLSATGGWFALRRRFPALAAGKLRFPNVRSAAARIFRR"
     gene            308..2392
                     /locus_tag="VF752_07935"
     CDS             308..2392
                     /locus_tag="VF752_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8855517.1"
                     /translation="MAFGGKGGSDLGRNTWVEIGLVVAAGLVIAAAVAYGPTRRLDGG
                     ASLLFFGALAALTALSVLWSIVPDATWSEANRTLAYLAVFAAGMGIARLAPGWWRVLL
                     WSLLLAATAVIVYALASRVFPGSIAPDELYARIGQPFGYWNAVGVTAAIAVPPGLWLG
                     ARRAGYAPANALAYPLLGLMFVALFLSYSRGALAATIVALGLWFLFVPLRLRSLGVLI
                     VSALGATPVIVWALSKDAFTKDLVPLATKESVAGTFGLLLLVMVAVLLAIGLAASFGL
                     GVRAPSPRFRRRIGLAAAVVACAIPLVGLTSVAFSQKGFTGTISDKWSQATSESAKTS
                     GGPGRLTQTSSSRGRYWRQAGHIFADHPLGGTGAGTFGTARLRYRNDVLVARHAHGFF
                     AQTMADLGVGGLAVVVAFAIAWLWAAARTLGIRISWRPWRRNANAPPREPLRWDAERV
                     GLAAITLAALAFGLHSAIDWTWFVPGPAVMAILAAGFVAGRGPVVEAAAVPAGGPRLS
                     LVGDGAAPPAGRRLRVPSPQRVAFALVALAAAGLIVWQTAQPERSDSQTQDAISLAAS
                     GKLTPAVTKAKAAAATDPLSPKPLLVEAAVLDAAGHPKEAKSTLEQAVIRFPSQPQVW
                     LRLADYDLHRAGDLPGATNALSGVLFLDPLNEQARELFLEARARQRGQKPGATAPAPP
                     PPAAEQAPAPGE"
     gene            complement(2396..3322)
                     /locus_tag="VF752_07940"
     CDS             complement(2396..3322)
                     /locus_tag="VF752_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8855518.1"
                     /translation="MTAEPTTARRGATLGAAFASRPVAAAVTGALTIAFSAILVRASH
                     ATPSTAAFFRCAYALPPLALLAWAEHRRYGPRSSRDLWLSLLAGAFFAADLICWHHAI
                     ADVGAGLATVLGNLQVVLVGVIAWVLLNERPERRLLVAIPVVLVGVVLISGAIGAGAY
                     GRDPARGVLFGVLTGLTYAGFILVLRHGNRDLRRPAGPLFEATAASALFCLAAGLVLG
                     DLDLIPTWPGHAYLITLALTSQVLGWLLISVSLPRLPAAVTSVVLTLQPVGSVLLGIA
                     IFSESPSPEQLLGVVVILAGVTYANLGRTRPA"
     gene            3442..3768
                     /locus_tag="VF752_07945"
     CDS             3442..3768
                     /locus_tag="VF752_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX8855519.1"
                     /translation="MRPHLGKTGAAVAVVAALGLGAAGIALASNGSGSGSGSRLDDGK
                     DLLPQATLSETQAIAAAQTAASGDLNEVDLEHASGRLVFNVDVGSKDVQVDAQSGKVV
                     SVNADD"
     gene            complement(3811..4506)
                     /locus_tag="VF752_07950"
     CDS             complement(3811..4506)
                     /locus_tag="VF752_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855520.1"
                     /translation="MRCIRAAVAAIAVLWVAMPGAAQCRAFEPGRDTAKVDNPWFPLK
                     PGTTLVYTGVKDGRPARDVVFVTDRTKTISGVRCVVVRDRLYSRGHLIERTSDYYAQD
                     RRGTVWYLGEDTAELDAKGRVKSTEGTWHAGVDGARAGIFMPRHPRVGETHRQEYYKG
                     HAEDHFRVVSLHARVSVPYGMFRNALETREWTPLEPGVLDAKRYARGIGEVSEESLKG
                     GDERLELVSVSDR"
     gene            complement(4517..5962)
                     /locus_tag="VF752_07955"
     CDS             complement(4517..5962)
                     /locus_tag="VF752_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8855521.1"
                     /translation="MRLPIQLRLTAWYVGLLALVIVALGAFVAVRLRADLTADTDHSL
                     RSSAAQIRSSYERAGTAGFTARSATVLRVLPGDSGAQLVTADGALARVLGPDVPRSAL
                     ITAAEARAAMAGRELLKTAHGRSDNEPFRVFATGVGHDGERSALVVASSLEGVDSAVH
                     RVIVLLLLAGPVVLLIAGLGGRLIARRALSPVARVTEQAERIELDRLDERVPVPPTSD
                     EIARLAVTLNRMLERLDRGVQEKRQLVADASHELRTPLAVMRSELDVALAYGALEPEA
                     ARVLASAREEVERMTWTVDNLLTLATVDEGHLELLKRDFPLRRVVDDVVAELAPVAAD
                     RGVTVSAEGDRGVVAADRDRIRQVLANLVDNAVKHSPEGGEVRIAVWEEPGEARLSVA
                     DDGPGIPEAALPYVFDRFYRVDAARKHDDGGSGLGLAICREIVAAHGGSVWAESQPGR
                     GSTFHVSLPAGGPKSFSDSSQVDGRTVAASN"
     gene            complement(5959..6651)
                     /locus_tag="VF752_07960"
     CDS             complement(5959..6651)
                     /locus_tag="VF752_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855522.1"
                     /translation="MGPYSTQMRILLVEDEPKMAAAIARGLRQEGYAVDTATTGDEAL
                     FQARVYDYDAIVLDVMLPGRDGFEVCRTLRDEERWAPVLMLTALDQVADRVNGLDSGA
                     DDYLVKPFSFGELLARVRALLRRRPRERPAVLCAAGVELDPASHTVSRDGEPVDLTAR
                     EFSLLRFMMEHPSDVLTRTSLLEHVWDENYVGPSNVVDVYVGYLRRKLERPFGRPLIR
                     TVRGVGYTLEGE"
     gene            complement(6683..7327)
                     /locus_tag="VF752_07965"
     CDS             complement(6683..7327)
                     /locus_tag="VF752_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8855523.1"
                     /translation="MTVNSRWVVTHTYGPRFAMMPGVPSRYDDELWELVPRDRGPPPT
                     TLCEFVKSLGEAEDVLDLGAGDGRLSACLRATRLTLADVSGVALERARERLPEARIVE
                     LEPDVPLPLPDSSFGLVLCAETLEHVRDVQLLLSEARRVLRPGGRLAVTTPAHGRGTA
                     LGLVARGFESGFDPLSPHLRFFTARSLRRLLGQMGFDVESLRRRRGTLYAVARR"
     gene            7345..7890
                     /locus_tag="VF752_07970"
     CDS             7345..7890
                     /locus_tag="VF752_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX8855524.1"
                     /translation="MAQQTDSTPAEAQLLVDFVNTLDIDDDIEQIGSPDELAAWLGEH
                     GLMQGGSARPEDVALAQRVRESLRALMHANNGEPLDPDAVDELNKLSVETPLVLRFSS
                     DGSTELAPGAEGAAAGLTRLMGNVYALMAEGKWQRVKACPADSCEWAFYDQSKNRSRT
                     WCSMEVCGNRSKARAYRRRHT"
     gene            7900..8535
                     /locus_tag="VF752_07975"
     CDS             7900..8535
                     /locus_tag="VF752_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855525.1"
                     /translation="MRALPAALALLALAAPAASGFFEPVSRHVDLTGDGKPERVHTVK
                     MPGPGGGTGDLEAQTAVAIADTCGDNQFVTRPQDSLGSLSFPKADTHRGREAFIDLRS
                     GASGRAGSIDLVALRPQGSGCPRPRELFRYSSDHPPRAPRGADGAAGFAAKVRDMSSR
                     FKGRELALSVYFTKPGEPLCCPSIRRTILYRYDSKRDRYVRYAARTTRRPN"
     gene            8611..9564
                     /locus_tag="VF752_07980"
     CDS             8611..9564
                     /locus_tag="VF752_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8855526.1"
                     /translation="MKRTTRLASAFVACLGLLGMLAAVALAATITGTNARDRITGTPS
                     GDTINALAGSDRVRALAGDDAVNGGPGRDLLWGGGGNDRLRGGPGSDIQLGGPGDDDV
                     GGGGGRDFLFGGAGNDNVHGWIGPDRIWGNAGDDTLNGGAAGRDRLWGGLGNDNITGG
                     RGNDRIHGGDGNDTDAGGAGNDLMSGGTGNDVQHGGDGNDVIFANLGADESFGDAGND
                     NLWALARGDVTADGDTTVDTLHGGDGNDTFHTRDGEADRIDCGPGNDTALLDEVDVIV
                     DATPENANGSCETVVRKAPKPKGEDRQENQTQSPSEDRKTH"
     gene            complement(9804..10379)
                     /locus_tag="VF752_07985"
     CDS             complement(9804..10379)
                     /locus_tag="VF752_07985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8855527.1"
                     /translation="MVARFGSSRREIPAERLEDDTILLRQPEERDVRWLVGMHQDSLI
                     PRFTGVPHPYSELDARAWIAAAPGRLSAGEGADFVIMDREDQRLLGTVGLVRVRWEDL
                     TAEVGYSLAAGARGHGYATRAVELVTRWAFDELGMRRMELTTHVDNARSQAVAERAGY
                     KREGVMRSYRVLRDRRVDLVMFSRLPRDPVP"
     gene            complement(10373..>11244)
                     /locus_tag="VF752_07990"
     CDS             complement(10373..>11244)
                     /locus_tag="VF752_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX8855528.1"
                     /translation="VSLKPIAENGLRDLGRLEEIERWFGQIDEVLIADPDFPQEEAVE
                     LTERCHRNGVRVRIAPSTMEILMDRVEFVPGQTLPLFELKAPVFEGVDFVVKRVFDLI
                     ASSLILLLLSPLLLLMALAVRLTSRGPVIYRSMRPGIGGEPFACLKFRTMYENAEHRQ
                     TELEKHNEAGGAIFKMRRDPRVTPVGRLLRRFSLDELPQLFNVLKGEMSLVGPRPLPQ
                     RDYERLGDWHRKRYLVLPGVTGLWQVSGRSELDFDELVRLDFVYLENWSVFLDITIIL
                     KTIPAVFRAKGAW"
BASE COUNT         1699 a         4099 c         3921 g         1525 t
ORIGIN      
        1 aagtcgctcg acagcgccac ccagaaggcg cgcagcggcg aagcgcccac gctctccgtc
       61 gcgggcagga cggtcgagcc gggcagcggc ggcgtcttca agacggcggc cgccgcgaac
      121 ggcgtgcccg gtccgatgct cgcggcactg atcggaatcg cggtgctcag cgcaacaggc
      181 ggctggttcg ccctccggcg gcgcttcccc gcgctcgccg caggcaagct ccgtttcccg
      241 aatgtccgca gcgcggcagc acgcatcttc cgccgctagc gtcgtcctcg gcgctgccct
      301 tgccgccgtg gcgttcggcg gcaagggcgg ctccgatctc ggccgcaaca cctgggtcga
      361 gatcggactg gtcgtcgccg cgggtctggt gatcgccgcg gcggtcgcct acgggcccac
      421 gcggcggctc gacggcggtg cctcgctgct cttcttcggc gcgctggccg cgctcacagc
      481 gctgtcggtg ctctggtcga tcgtgcccga cgcgacctgg agcgaagcga accgcacgct
      541 cgcctacctc gccgtgttcg cggccgggat gggcatcgcg cggctcgcgc ccggctggtg
      601 gcgcgtactg ctctggagcc tcctgctcgc cgccaccgcc gtgatcgtct acgcgctcgc
      661 ttcccgcgtg ttcccgggca gcatcgcgcc cgacgagctg tacgcgcgca tcggtcagcc
      721 gttcggctac tggaacgcgg tgggcgtgac ggcggcgatc gcggtgccgc cgggcctctg
      781 gctcggcgcg cgccgggccg gctacgcgcc tgcgaacgcg ctcgcctacc cgctgctcgg
      841 gctcatgttc gtggcgctct tcctcagcta ctcgcgcggc gcgctcgctg ccacgatcgt
      901 cgcgcttggc ctctggttcc tgttcgtgcc gctgcgactg aggagcctgg gcgtgctcat
      961 cgtctccgcg ctcggggcca cgccagtgat cgtctgggcg ctctcgaagg acgccttcac
     1021 caaggacctc gtgccgctcg cgaccaagga gagcgttgcc ggcacgttcg gcctgctcct
     1081 gctggtgatg gtggcggtgc tgctggcgat tgggctcgcg gcgagcttcg gcctcggcgt
     1141 gcgcgcgccg tcgccgcgct tccgccgcag gatcgggctg gccgccgccg tggtcgcctg
     1201 cgcgatcccg ctcgttgggc tcacctcggt cgcgttcagc cagaagggct tcacgggcac
     1261 gatctcggac aagtggtcgc aggccacgag cgagtcggcg aagacctcgg gcgggccggg
     1321 gcgcctcacc cagacctcct cttcgcgggg gcgctactgg cgtcaggccg gtcacatctt
     1381 cgccgaccac ccgctcggcg gcaccggcgc cggtacgttc ggcacggcgc gactgcggta
     1441 ccgcaacgac gtcctggtgg cgcggcacgc gcatggcttc tttgcgcaga cgatggccga
     1501 cctcggcgtg ggcgggctgg ccgtggtggt cgcgttcgcg atcgcctggc tgtgggcggc
     1561 cgcgcgcacc ctcgggatcc ggatcagctg gcgtccgtgg cggcgcaacg ccaacgcgcc
     1621 gccgcgcgag ccgcttcgct gggacgccga gcgggtcggc ctggccgcca tcacgctcgc
     1681 cgcgctcgcg ttcggcctcc actcggcgat cgattggacg tggttcgtgc cgggtccggc
     1741 ggtgatggcg atcctcgcgg ccggcttcgt cgccgggcgc gggccggtgg tggaggctgc
     1801 ggccgtaccc gccggaggac cgcggctctc gctggtgggc gacggcgccg cgccgccggc
     1861 cgggcggcgc ctgcgcgtgc cttctccgca gcgggtggcg ttcgcgctcg tcgcgctcgc
     1921 tgcggccggc ctgatcgtct ggcagacggc gcaacccgag cgctcggact ctcagaccca
     1981 agacgcgatc tccctggctg cgagcggcaa gctcaccccg gcggtgacca aggcgaaggc
     2041 ggccgccgcc acggacccac tgtcgccgaa gccgctgctt gtggaagcgg ccgtcctcga
     2101 cgcggcgggg cacccgaagg aggcgaagtc cacgctcgag caggccgtga tccgcttccc
     2161 cagccagccg caggtttggc tgcgcctcgc cgactacgac ctccaccgcg cgggcgactt
     2221 gcccggtgcg acgaacgcgc tcagtggagt gctcttcctc gacccgttga acgagcaggc
     2281 gcgcgagttg ttcctcgagg cgcgggcgcg gcagcgtggc cagaagccgg gtgccaccgc
     2341 gccggcaccg ccgccgcccg cggccgagca ggcgcccgcg cccggcgagt agcgctcagg
     2401 cgggccgcgt gcggcccagg ttcgcgtagg tcacaccggc gaggatcacg accacgccga
     2461 gcagttgctc cggcgatggc gactcgctga agatcgcgat gccgagcagc accgagccga
     2521 ccggctgcag cgtgagcacc accgacgtca ccgcggccgg aagccgcggc agcgacacgg
     2581 agatcaggag ccagccgagt acctgcgacg tgagcgccag cgtgatcagg tacgcgtgcc
     2641 ccggccaggt cgggatgaga tcgaggtcac cgagcacgag cccggccgcg aggcagaaca
     2701 gcgcggaagc ggcggtggcc tcgaacagcg gaccggcggg tcgccgcagg tcccggttgc
     2761 cgtgcctcaa cacgaggatg aagcccgcgt aggtgagccc cgtgagcacg ccgaacagca
     2821 cgccccgcgc cgggtcccgg ccgtaggcgc cggcgccgat cgcgccggag atcagcacca
     2881 cgcccacgag caccaccggg atcgcgacga gcaaccgccg ctcggggcgc tcgttgagga
     2941 gcacccaggc gatcactccg accagcacga cctggaggtt gccgagcacg gtggcgagcc
     3001 cggcccccac atcggcgatg gcgtgatgcc agcagatgag atcggcggcg aagaaggctc
     3061 cggcgagcag cgacagccac aggtcgcggc tgctccgcgg cccgtagcgg cgatgctcgg
     3121 cccacgcgag cagcgccagc ggcggaagcg cgtaggcgca tcggaagaac gccgccgtgc
     3181 tcggcgtggc gtgcgacgcg cgcacgagga tcgccgagaa ggcgatcgtg agcgcacccg
     3241 tcacggccgc cgcgacgggt ctactcgcga aggccgcgcc gagcgtggcg ccgcggcggg
     3301 cggtcgtcgg ctctgcggtc acggcggccg acgttagtgc agcgcgccgc gcgcctgtct
     3361 catccgtgtc tcatgctccc ctcagagccc cctctctaac ttcgcgggca ctgttcggaa
     3421 accggctacg gaaggaggct catgcgacct cacctcggca agaccggcgc agccgtcgcg
     3481 gtagtggcgg ccctcggcct cggtgccgcc ggcatcgcgc tcgcgtcgaa cgggtctgga
     3541 tcaggatcag ggtcgcgcct cgacgacggg aaggacctgc ttcctcaggc gacgctcagc
     3601 gagacccagg cgatcgcggc cgcgcagacc gcagcgtccg gcgacctcaa cgaggtcgac
     3661 ctcgagcacg cgtccgggcg gctggtgttc aacgtggacg tcggcagcaa ggacgtccag
     3721 gtcgacgcgc agtccgggaa ggtcgtcagc gtcaacgccg acgactagtg gcgatcggga
     3781 ggggcggcca acgccgtccc tccgtcaccc tcagcggtca ctcacgctga cgagttcgag
     3841 cctctcgtcg ccgcccttga gcgactcctc agatacctcg ccgatcccgc gcgcatagcg
     3901 cttcgcgtcg agcacgcctg gctcgagcgg ggtccactcc ctcgtctcga gcgcgttgcg
     3961 gaacatgccg tacggcacgg agactcgcgc gtgcaggctc accacacgga agtggtcctc
     4021 ggcgtggccc ttgtagtact cctgccggtg cgtctcgccg acccgtggat ggcgcggcat
     4081 gaagatgccg gcgcgcgcgc cgtcgactcc cgcgtgccac gtgccttcgg tgctcttcac
     4141 ccggcccttt gcgtcgagct cagcggtgtc ctcgccgagg taccagaccg tgccgcggcg
     4201 gtcctgggcg tagtagtccg atgtccgctc aatcaggtgt ccgcgtgagt acagccggtc
     4261 gcgtacgact acgcaccgca cgccgctgat cgtcttcgtc cgatcggtga cgaacaccac
     4321 gtcgcgcgca ggccggccgt ccttcacgcc cgtgtagacc agcgtcgtgc caggcttcag
     4381 cgggaaccac gggttgtcga ccttcgcggt gtcccttccg ggctcgaagg cgcggcactg
     4441 tgccgcgccg ggcatcgcga cccacagcac cgcaatagcg gcgacggctg ctcgtatgca
     4501 tctcatcgga tcctcctcag ttggacgcgg cgaccgtacg cccgtctacc tgagaggaat
     4561 ctgagaacga cttcgggccg ccggccggca ggctcacgtg gaaggtgctg ccccggcccg
     4621 gctggctctc ggcccacaca cttccaccat gagcggccac gatctcgcgg caaatcgcga
     4681 gccccaggcc gctgcccccg tcgtcgtgct ttcgcgccgc gtcgacacgg tagaagcggt
     4741 cgaacacgta tggcagtgcg gcttcgggta tgcccgggcc gtcgtcggcc acgctcaagc
     4801 gcgcctcccc cggctcctcc cacaccgcga tccgcacctc gccgccctcc gggctgtgct
     4861 tcaccgcgtt gtcgacgagg ttcgcgagaa cctgccggat ccgatcgcgg tcggctgcta
     4921 cgacaccacg gtcaccctcg gccgatacgg tcacgccgcg gtccgccgct accggcgcga
     4981 gctctgccac cacgtcgtcc accacgcgcc gcaaggggaa gtcgcgcttg agcagctcga
     5041 gatggccctc gtcgacggtc gcgagcgtga ggaggttgtc gaccgtccac gtcatccgct
     5101 cgacctcctc gcgcgcactc gcgagcacgc gtgccgcctc cggctcgagg gcgccgtagg
     5161 caagcgcgac gtcgagctcc gagcgcatca cggcgagcgg cgtgcgaagc tcgtgcgagg
     5221 cgtcggccac gagctgccgc ttctcctgaa ctccgcgatc gagccgctcc agcatccggt
     5281 tgagggtgac ggcgaggcgc gcgatttcgt cagaggtcgg cggcaccggc acccgctcgt
     5341 cgaggcgatc gagctcgatg cgctccgcct gctccgtcac gcgcgcgacc gggctgagag
     5401 ccctgcgcgc gatcaggcga ccgcccagtc ccgcgatcag cagcaccacc gggcccgcaa
     5461 gcagcagcag cacgatcacg cggtgcaccg cggaatcgac tccctcgagc gagctcgcca
     5521 ccacgagcgc cgagcgctcg ccgtcgtgac ccacgccggt ggcgaacacg cggaacggct
     5581 cgttgtcgga gcgcccgtgc gcggtcttga ggagctcgcg tccggccatc gcggcgcgcg
     5641 cttcggccgc cgtgatgagg gccgagcgcg gcacgtcggg accgagcacg cgcgccaggg
     5701 ccccgtcggc ggtcacgagc tgcgcgccag aatcgccggg cagcacccgc agcacggtgg
     5761 cgctacgggc agtgaacccc gcagtgccgg cgcgctcgta gctcgagcgg atctgggcgg
     5821 cgctcgagcg cagcgagtga tcggtatcgg cggtcagatc ggctcgcagc cggaccgcga
     5881 cgaaggcgcc gagcgccacg atcacgaggg ccagcaggcc cacgtaccag gcggtcagcc
     5941 ggagctggat cggcagcctc actccccctc cagcgtgtag ccgaccccgc gaaccgtccg
     6001 gatcagcggc cgcccgaacg gccgctccag cttcctgcgc agatagccca cgtacacgtc
     6061 caccacgttc gacgggccga cgtagttctc gtcccagacg tgctcgagca ggctcgtgcg
     6121 agtgagcacg tcgctggggt gttccatcat gaaccgaagc agcgagaact cgcgcgccgt
     6181 gaggtccacg ggctcgccgt cgcggctgac ggtgtggcta gccggatcga gctccacgcc
     6241 ggcggcgcac agcaccgccg ggcgctcgcg cgggcgcctg cgcagtagcg cgcgcacgcg
     6301 cgccagaagc tcgccgaacg agaacggctt caccaggtag tcgtcggcgc ccgagtcgag
     6361 cccgttcacg cggtccgcaa cctggtcgag cgccgtcagc atcagcaccg gcgcccaccg
     6421 ctcctcatcg cgcagcgtcc ggcacacctc gaagccgtct cggcccggca gcatcacgtc
     6481 gagcacgatg gcgtcgtagt cgtagacccg cgcctggaag agcgcctcgt cgcccgtcgt
     6541 ggccgtgtcg acggcatagc cctcttgccg gagcccgcgg gcgatcgcgg ctgccatctt
     6601 cggctcgtcc tcaacgagca ggatgcgcat ctgcgtcgag tatggaccca cccgggtgag
     6661 agagcgatga ggaactcgcg cctcagcgcc gggcaaccgc gtacagcgtg ccgcgccggc
     6721 gcctcagcga ctcaacgtcg aagcccatct gcccgagcag ccgccggagc gacctcgctg
     6781 tgaagaagcg caggtgcggc gagagcgggt cgaagccgct ttcgaacccg cgcgcgacaa
     6841 gcccgagcgc ggtgccccgt ccgtgcgcgg gagtggtgac cgcaaggcgg ccgcccggtc
     6901 tcaacacgcg ccttgcctcc gagagcagga gctgcacgtc gcgcacgtgc tcgagcgtct
     6961 cggcgcaaag caccaggccg aacgacgagt cgggcagcgg caacggcacg tcgggctcga
     7021 gctcgacgat gcgcgcctcc ggcagccgct cgcgcgcgcg ttcgagagcc acgcccgaca
     7081 cgtccgcgag cgtcaacctc gtcgcacgca ggcaggcgct gaggcggccg tcgcccgcgc
     7141 cgaggtcgag cacgtcctcc gcctccccca gcgacttcac gaactcgcag agcgtggtgg
     7201 gcggcgggcc ccggtcgcgg ggcacgagct cccacagctc gtcgtcgtag cggctcggca
     7261 cgccgggcat catcgcgaag cgcggcccgt acgtgtgcgt caccacccat cgactgttga
     7321 cggtcacggg tctaaacttg cagcatggcc cagcagaccg acagcacgcc cgccgaggcg
     7381 cagctcctcg tcgacttcgt caacacgctc gacatcgacg acgacatcga gcagatcggc
     7441 tcgcccgacg agctagcggc gtggctcggc gagcacgggc tgatgcaggg cggctcggcc
     7501 cgccctgagg acgtcgcgct cgcccagcgc gtgcgcgagt cgctgcgcgc gctcatgcac
     7561 gcgaacaacg gcgagccgct cgatccggac gcggtcgacg agctgaacaa gctctccgta
     7621 gagacgccgc tcgtgctgcg cttcagctcc gacgggtcga ccgagctcgc gccgggggcg
     7681 gagggcgccg ccgccggcct cacgcgcctg atgggcaatg tctacgcgct gatggccgag
     7741 ggcaagtggc agcgggtgaa ggcgtgccct gccgacagct gcgagtgggc gttctacgac
     7801 caatcgaaga accgctcgcg cacctggtgc agcatggaag tctgcggcaa ccgcagcaaa
     7861 gcccgcgcat atcgccgccg ccacacgtag agtcgcgaca tgcgtgcact gccggcggca
     7921 ctcgccctgc tcgccctggc cgcgcctgcc gcgtctggct tcttcgaacc ggtcagccgc
     7981 cacgtggatc tgacgggcga cggcaaaccc gagcgcgtgc acacggtgaa gatgcccggc
     8041 ccgggcggcg ggaccggcga cctcgaggcg cagaccgcgg tcgccatagc ggacacatgc
     8101 ggcgacaacc agttcgtcac gaggcctcag gattcgctcg gctcactcag tttcccgaag
     8161 gcggacaccc accgtgggcg cgaggcgttc atcgacctgc gctctggcgc gtcgggccgc
     8221 gccggctcca tcgacctcgt cgcgctgcgt ccacagggca gcggctgccc gcggccgcgc
     8281 gagctgttcc gctactcgag cgatcacccg ccccgcgcgc cgcgcggcgc tgacggggcg
     8341 gccgggttcg cggccaaggt gcgcgacatg agctcccgct tcaagggccg ggagctcgcg
     8401 ctgagcgtgt acttcacgaa gcccggcgag ccactctgct gcccgagcat tcggcggacg
     8461 atcctctacc gctacgacag caagcgcgac cgctacgtgc gttacgccgc gcgcaccacg
     8521 cgccgaccca actaggcgcg ccgcaactct tgaccggtgc accgtgttct acgggcacca
     8581 agacgcgata cccccaaggg gagggttcaa atgaagcgaa cgactcgcct cgcgagcgct
     8641 ttcgtcgcgt gcctggggct tctaggcatg ctcgcagcgg ttgcgctcgc agcgacgatc
     8701 acagggacga atgcacgaga caggatcacc ggtacgccgt cgggcgacac catcaacgcg
     8761 cttgccggga gcgacagggt ccgcgcgctc gcgggtgacg acgcggtgaa cggcggacct
     8821 ggccgtgacc tcctttgggg cggcggcggc aacgacaggc tgcgcggtgg gccgggcagc
     8881 gacatccagc tcggtggccc cggcgacgac gacgtgggtg gcgggggcgg ccgggacttt
     8941 ctgttcggcg gagcgggcaa cgacaacgtg cacggctgga tcggccccga ccgcatctgg
     9001 ggcaacgccg gcgacgacac gctgaacggc ggagccgccg gcagggaccg cctgtggggc
     9061 ggcctgggca acgacaacat caccggcggc aggggcaacg accgcatcca cggcggtgac
     9121 ggcaacgaca ccgacgcggg cggggccggc aacgatctga tgtccggcgg caccggcaac
     9181 gacgtccagc acggcggcga cggcaacgac gtcatcttcg ccaacctggg cgccgacgag
     9241 tcgtttggcg acgcgggcaa cgacaacctc tgggcactgg cccgaggcga cgtgaccgcc
     9301 gacggcgaca ccaccgtcga cacgctccac ggcggcgacg gcaacgacac gttccacacg
     9361 cgcgacggcg aggccgaccg gatcgactgc ggtcccggca acgacaccgc gctcctcgac
     9421 gaagtcgacg tgatcgtgga cgccaccccg gagaacgcga acggctcgtg cgagacggtg
     9481 gtccgcaagg caccgaagcc gaagggcgag gatcgtcagg agaaccagac gcagagcccc
     9541 tcagaggacc gaaagaccca ctagcgccca ggacgggcga acgtggatct ccgccccggc
     9601 ccagggggcg gagccctacg agtcaggcga gggcgcttct tcggaggcgc cctctccgct
     9661 cttcgaggca atgagggtga gggaacaccg ggagtttccg ctctaccgtg gtcgattttg
     9721 ccgccatatg cggcactaac gaccacggtg cgcgaaagtc cgccgccggg cgcaggatcc
     9781 ggcgggctta gacccggcgg cggttagggg acggggtcgc ggggcagcct cgagaacatc
     9841 acgaggtcca cgcgccggtc gcgcaacacc cggtacgagc gcatcacgcc ctcacgcttg
     9901 tagccggccc gctcggcgac agcctgggag cgcgcgttgt ccacgtgcgt ggtcagctcc
     9961 atccgccgca tgccgagctc gtcgaaggcc cagcgcgtca ccagctcgac cgcgcgcgtc
    10021 gcatagccgt gtccgcgcgc acccgcggca agcgagtagc ccacctcggc ggtcagatcc
    10081 tcccaccgca ctcgcaccag ccccaccgtg ccgaggagac gctggtcctc gcgatccatg
    10141 atcacgaagt cggcgccttc gcccgccgaa agccggccgg gcgcggctgc gatccaggca
    10201 cgcgcgtcga gctccgagta gggatgcggc acgcccgtga agcgagggat cagcgaatct
    10261 tggtgcatcc ccacgagcca gcgcacgtcg cgctcctcag gctggcgaag gaggatcgtg
    10321 tcgtcctcca gccgctcggc cgggatctcc cggcgactgc ttccgaagcg ggctaccagg
    10381 cgcccttcgc gcggaacacg gctggaatgg tcttcaggat gatcgtgatg tcaaggaaga
    10441 ccgaccagtt ctcgaggtac acgaagtcga gccgcacgag ctcgtcgaag tcgagctccg
    10501 agcgccccga cacctgccac aggccggtca cgcccggcag cacgaggtaa cgcttgcggt
    10561 gccaatcccc gagccgctcg tagtcgcgct gcggcagcgg gcgcgggccg acgagcgaca
    10621 tctcgccctt cagcacgttg aacagctgcg gcagctcgtc gagcgagaag cgccgcagga
    10681 gccggcccac aggcgtcaca cgcgggtcgc gccgcatctt gaagatcgcg cctccggcct
    10741 cgttgtgctt ctcgagctcc gtctgccggt gctccgcgtt ctcgtacatc gtgcggaact
    10801 tcaggcacgc gaagggctcg ccaccaatgc cggggcgcat cgagcggtag atcaccggcc
    10861 cgcgcgaggt gagcctcacc gcgagcgcca tcagcagcag gagcggcgag agcagcagca
    10921 ggatgaggct cgaggcgatc agatcgaaga cgcgcttcac gacgaagtcg acgccctcga
    10981 agaccggcgc cttcagctcg aacagcggaa gcgtctggcc gggcacgaac tccacgcggt
    11041 ccatgaggat ctccatcgtg gagggcgcga tccgcacgcg cacaccgttg cggtggcagc
    11101 gctcggtgag ctcgacggcc tcctcctgcg ggaagtcggg gtccgcgatc agcacctcgt
    11161 cgatctgccc gaaccagcgc tcgatctcct cgagccggcc gaggtcgcgc aggccgttct
    11221 cggcgatcgg cttcagtgac acga
//
LOCUS       DASVXS010000145         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1171854, whole genome shotgun sequence.
ACCESSION   DASVXS010000145 DASVXS010000000
VERSION     DASVXS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171854"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VF752_07995"
     CDS             complement(<1..510)
                     /locus_tag="VF752_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855529.1"
                     /translation="MATPEAQAVISRRRLIPAYVALLCAGSLTVAAFADRSEVGDHPV
                     LFVLLIAFVLLVDGLRIDLFERGGTSPAGIPTLALAFLFGPIGPIASEASIAVVRLVR
                     GTQPIKALYDFGSLSLAGAAVAALLTLLPNEHNFAVMAGLAGASLVYYFANSAALGTI
                     WWLDEGISPM"
     gene            643..849
                     /gene="rpmE"
                     /locus_tag="VF752_08000"
     CDS             643..849
                     /gene="rpmE"
                     /locus_tag="VF752_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX8855530.1"
                     /translation="MKTEIHPEYVEAHVRCTCGNEFTTRSTQPEIHVEICSNCHPFYT
                     GRQKLVDTGGRVERFRRRAAKRAR"
     gene            917..1669
                     /locus_tag="VF752_08005"
     CDS             917..1669
                     /locus_tag="VF752_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8855531.1"
                     /translation="MARRSQLILVGCAAFAALPASSALAAGASPNARVGNCYPSPDIT
                     KRFAEFVGQMKTIRGANSMQMRFTLQERVGGARFHAVALPELKVWRKSKPGARAFNFT
                     QRVTALRDGARYRVIVKFRWLDTNGKRLRATTRRSASCFQPGPLPNLKITAIQADPGP
                     LPDSVVYSITMVNNGRAIAFKPAAVVNVDGAELDQGGLAQLGPGESGVVRFSGPFCRF
                     LVRAKADPSGLIPEEDERDNVLTTPCPFGSKP"
     gene            1688..2275
                     /locus_tag="VF752_08010"
     CDS             1688..2275
                     /locus_tag="VF752_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855532.1"
                     /translation="MRSLAWRLRAAALVLAGALAVHELRYLVAYQGDSGRALSLQGHA
                     YLSLVTPLVCALIAVAAAHFVGRLARGGSDEATPGFAATWLWASLSLVAIFVVQESVE
                     GLVASGHPGGLAAVVGHGGWLAAPIAFAVGGVIALLLRGASVALAAAARRARPPVSRV
                     TPLVRRPPLGVTRPRQHPLALKLAGRAPPRASVLA"
     gene            2300..2749
                     /locus_tag="VF752_08015"
     CDS             2300..2749
                     /locus_tag="VF752_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855533.1"
                     /translation="MNQRGRRLAILVVGVIAIVVAFVVLNSSGNDNNDNKQASSTTST
                     TATTPGQSTTQNTSASAQPAVKTVVVKNAKPVGGIQKLSFNKGDQVRFRVKSDVGDEI
                     HVHGYDFHKDVKPGSSVTFSFPAKIDGIFIVELESRGEQIASLQVNP"
     gene            2749..4164
                     /locus_tag="VF752_08020"
     CDS             2749..4164
                     /locus_tag="VF752_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855534.1"
                     /translation="MRRRLALIAVGVAAASLAVPAPALAHGLVGRADLPIPSWLFGWA
                     AAAVLIVSFVALATLWPEPRLQEPRLRRLFRTPAVIEVVCGLIGVFLFGLVVYAGLRG
                     TQTATANLAPTFIYVHFWVGLVVLSVLFGDVFRLFNPWLAIGRAVSWVAGKTLGTEEG
                     DALEYPSWLGRWPAVAGILGFAWLELAYVNRDDPSTLAILSLGYALVQLVGMGLFGVE
                     TWSRRGDAFGVYYGMLSRLSPFERREGVLYLRPPLSGAPRLDAEIPGTLAVVLTAIGT
                     TTFDGFSSGPVWRKLEPHLSNFWSNLGADPAHALQWSFTVGLVVIVLLVSSFYWLGIR
                     GMHTIDEKNSTQELARRFAHSLIPIAFAYALAHYFSLLIYQGQAAAYLASDPLGHGSN
                     IFGTANKGIDYGVITATGIWYVQVAALVIGHISGLVLAHDRAVAMYRDVREATRSQYW
                     MLVVMIGFTSLGLWLLSAINQ"
     gene            4161..4313
                     /locus_tag="VF752_08025"
     CDS             4161..4313
                     /locus_tag="VF752_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855535.1"
                     /translation="MSTLTLLLPFAHAGHVLVTLPIYLGPVILLVLGLKVADARDKRK
                     KANRGR"
     gene            4364..>4581
                     /locus_tag="VF752_08030"
     CDS             4364..>4581
                     /locus_tag="VF752_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855536.1"
                     /translation="MSEVAESAARNGAAPESAPLESPRDLPVGGQAVLEGVMMRGVST
                     WAVAVRKPTPEQIAGGRQLEPTEAALGE"
BASE COUNT          679 a         1571 c         1584 g          747 t
ORIGIN      
        1 catcggcgag atgccctcgt cgagccacca gatcgtgccg agcgcggcgg agttcgcgaa
       61 gtagtagacg agcgacgcgc cggcgaggcc agccatcacg gcgaagttgt gctcgttcgg
      121 aagcagtgtc agcagcgcgg ccaccgcagc gcccgcgagc gacagcgagc cgaagtcgta
      181 gagcgccttg atcggttgcg tgccgcgcac gaggcggacc acagcgatgg acgcctccga
      241 ggcgatcggg ccgatcggcc cgaacaggaa cgccagggcc agcgtcggga tgccggccgg
      301 cgaggtgccg ccgcgctcga acaggtcgat gcgcagcccg tccacgagca gcacgaaggc
      361 gatcagcaga acgaacagaa ccgggtggtc cccgacctcg ctgcgatcgg cgaacgcggc
      421 gaccgtgagc gagcccgcgc agagcagcgc gacgtaggcg gggatcagcc gcctccgtga
      481 gatcaccgct tgcgcttcag gcgtggccat tcgcctcagt ttctgcatcg tcagctggcg
      541 ctcaaccttg agcgcgccgg ctggtgtcgg tattcgccgc gggaggcgcg ttgcctttcg
      601 agagcgggct atggactggg cgcccccgct agactcgaat tcatgaagac cgagatccat
      661 ccggagtacg tcgaggcgca cgttcgctgt acctgcggca acgagttcac gacgcgctcc
      721 acgcagcccg agatccacgt cgagatctgc tcgaactgcc atccgttcta cacgggccgg
      781 cagaagctcg tggatacagg cggccgggtg gagcggttcc gccggcgcgc cgccaagcgc
      841 gcgcgctaaa gcggccggct ccacagccgc ggccgctgcg caacttcccg ccgacactta
      901 gtgttgaaga tgggccatgg cgcgaagaag ccagctcatc ctcgtcgggt gcgcagcgtt
      961 cgcggcgctg cctgcgtcct cggcgcttgc cgccggggcc agtccgaacg ctcgcgtcgg
     1021 caactgctac ccctcgccgg acatcacgaa gcgtttcgcc gagttcgtcg ggcagatgaa
     1081 gacgatccgt ggggcgaatt cgatgcagat gcgcttcacg cttcaggaga gggtcggcgg
     1141 agcccggttc cacgccgtcg cgctgcccga gctgaaggtc tggcgcaagt cgaagccagg
     1201 cgcgcgtgcg ttcaacttca cccagcgcgt taccgcgctc cgggacgggg cgcgctaccg
     1261 cgtgatcgtc aagttccgct ggctcgacac caacggcaag cggcttcgcg ccacaactcg
     1321 gcgttccgcg tcctgcttcc agccgggccc gctgccgaac ctgaagatca ccgcgatcca
     1381 ggcggacccc ggcccgctac cggactcggt cgtctactcg atcaccatgg tcaacaatgg
     1441 gcgcgccatc gcgttcaagc ccgctgccgt ggtcaacgtg gacggcgccg agctcgacca
     1501 aggcggcttg gcccagctcg ggcctggcga aagcggagtc gtgcgcttct cgggcccgtt
     1561 ctgccgcttc ctcgtgcgtg cgaaggccga cccgagcggg ctgatccccg aggaggacga
     1621 gcgcgacaac gtgctcacca cgccgtgtcc attcggttcg aagccgtagc ggcctaaaat
     1681 ccccgccgtg cgctcgctcg cctggaggct tcgcgcggct gcgctcgtgc tggctggcgc
     1741 gctggccgta cacgagttgc gttacctggt cgcctatcag ggcgactccg ggcgagccct
     1801 gagcctccag ggtcacgcct acctgagcct cgtcacgccg ctggtgtgcg ctttgatcgc
     1861 cgtggcggcc gctcacttcg ttgggcggct cgcgcgcggc ggctctgacg aggcgacacc
     1921 cgggtttgcc gctacgtggc tatgggcgtc gctctcgctc gtcgcgatct tcgtggtgca
     1981 ggagtcggtc gagggcctgg tcgcaagcgg tcaccctggc gggctggccg cggtcgtcgg
     2041 ccacggcggc tggctggcgg cgccgatcgc cttcgccgtt ggtggcgtga tcgcactcct
     2101 cctgcggggc gcctcggtcg cgctagcggc ggcggcgcgc cgggcccgcc cgcccgtgtc
     2161 gcgcgtgaca cccctcgtgc gccgtccgcc gctgggcgtg acgcggccga ggcagcatcc
     2221 gctggcgctc aagctggccg gccgcgctcc gccgcgcgcc tccgtgctgg cctgaacgtc
     2281 aacgaaacgg aggtaatgca tgaaccagcg aggcagacgc cttgccattc tcgtcgtcgg
     2341 tgtgatcgcg atcgtcgtgg ctttcgtcgt cctgaactcg agcggcaacg acaacaacga
     2401 caacaagcag gcgagcagca ccaccagcac gactgcgacc acgccggggc agagcacgac
     2461 acagaacacg agcgcgtccg cgcagccggc ggtcaagacg gtcgtggtga agaacgccaa
     2521 gccggtgggc ggcatacaga agctcagctt caacaagggc gaccaggtgc gcttcagggt
     2581 caagtcggac gtgggggacg agatccacgt gcacggctac gacttccaca aggacgtgaa
     2641 gccgggcagc tcggtcacgt tctccttccc ggcaaagatc gacggcatct tcatcgtgga
     2701 gctcgagtcg cgcggtgagc agatcgcctc tctccaggtg aacccgtgat gagaaggcgg
     2761 ctggcactga ttgcggtcgg cgtcgctgcg gcgtcgctgg ccgtgcccgc gcccgcgctc
     2821 gcgcacgggt tggtgggccg cgcggatctg ccgatcccgt cgtggctgtt cggctgggcg
     2881 gccgcggccg tcctgatcgt gtcgtttgtc gccctcgcca ccctgtggcc ggagccgcgg
     2941 ctgcaggagc cgcgcctgcg gcggctcttc cgcacaccgg ccgtgatcga ggtcgtctgc
     3001 gggctgatcg gcgtgttcct gttcgggctc gtggtctacg cgggcctgcg cggcacgcag
     3061 accgcgacgg cgaatctcgc gcccacgttc atctacgtgc acttctgggt gggcctggtc
     3121 gttctgagtg tgctgttcgg cgacgtcttc cggctcttca acccgtggct cgccatcggt
     3181 cgcgccgtgt cctgggtcgc aggaaagacg ctcggcaccg aggagggcga cgcgctcgag
     3241 tacccctcat ggctgggccg ctggcccgcg gtcgcgggca tcctcggctt cgcctggctc
     3301 gaactcgcct acgtgaatcg cgacgatccg agcacgctcg cgatcctgtc tctgggctac
     3361 gcgctcgtcc agctcgtcgg catggggctg ttcggcgtgg agacgtggag ccggcgcgga
     3421 gacgccttcg gcgtctacta cggcatgctg tcgcgcctct cgccgttcga gcggcgcgag
     3481 ggagtgctct acctgcggcc gccgctcagc ggcgcaccac ggctcgacgc ggagattccg
     3541 ggaacgctcg cggtggtgct gaccgcgatc ggcacgacga cgttcgacgg cttctcgagc
     3601 ggcccggtgt ggcggaagct cgagccgcac ctgtcgaact tctggtcaaa cctcggcgcc
     3661 gacccggccc acgcgctgca gtggtccttc acggtggggc tcgtcgtgat cgtgttgctc
     3721 gtgtcgtcgt tctactggct cgggatccgc ggcatgcaca cgatcgacga gaagaactcc
     3781 acccaggaac tcgcgcgccg gttcgcacat tcactgatcc cgatcgcgtt cgcctacgcg
     3841 ctcgcgcact acttctcgct gctgatctac caggggcagg cggctgcgta cctggcgtcg
     3901 gacccactcg ggcatggctc caacatcttc ggcacggcga acaagggcat cgactacggc
     3961 gtgatcacgg cgacggggat ctggtacgtg caggtcgccg cgctcgtcat cggccacatc
     4021 tcgggcctgg tgctggcgca cgaccgcgcg gtcgcgatgt acagggatgt tcgcgaggcg
     4081 acacgctcgc aatactggat gctggtcgtg atgatcggct tcacgagcct cggactctgg
     4141 ctcctttccg ccatcaacca atgagcacgc tcactctcct cctgccgttc gcccacgcag
     4201 gccatgtgct cgtcacgctg ccgatctacc tcggcccggt gatcctgctc gtgctggggc
     4261 tgaaggtggc cgacgcgagg gacaagcgaa agaaggcgaa ccgggggcgc tgaggcgccc
     4321 ggttgcttgc gggaacccgc caaaccgccg atagagtgag ggcatgagcg aggtcgccga
     4381 aagcgccgct aggaacggcg ccgctcctga gtccgcgccg ctcgagtcgc cccgcgacct
     4441 cccggtcggg ggtcaggccg tgctcgaggg cgtgatgatg cgcggcgtgt ccacgtgggc
     4501 cgtcgcggtc cgcaagccga cgcccgagca gatcgccggc ggccgccagc tcgagccgac
     4561 cgaggcggcg ctcggcgaga t
//
LOCUS       DASVXS010000146         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1080954, whole genome shotgun sequence.
ACCESSION   DASVXS010000146 DASVXS010000000
VERSION     DASVXS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080954"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VF752_08035"
     CDS             <1..569
                     /locus_tag="VF752_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855537.1"
                     /translation="AVPNMRAGALLPHGAIHDPGRLDRLGISPIVNLHVVYDRPVLQH
                     AFVAGVRSPVQYVFDRTREGEFGGGQYLAVSLSGAAAEMERPPEELRRQYVPALAEIL
                     PAARNAKVERFFANREHAATFRAAPGQLALRPPSRTRIPGLALAGAWTATGWPATMEG
                     AVRSGHTAARVALEALDSPRPAPAGVAA"
     gene            566..2107
                     /locus_tag="VF752_08040"
     CDS             566..2107
                     /locus_tag="VF752_08040"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8855538.1"
                     /translation="MIGLRPEPRAAEHEGLLVLAPLSLEARAVRSGVGRGRVLRTGMG
                     PERAVEAVKTPVAREADAVAVAGFCGSLDAGLEPGDVVVATEVRGPDGAVECPSAGVL
                     AGVLERHGVRAVCGPIVSTDHVVQGDERRELAASGALAVDMESAQLAAGARDRPIAVL
                     RVVVDTPDRELRDPLATVTGGLRAYRALARASKALGEWAAAVGPRRLMLASPRASCAG
                     VERAIEVVERALEQHGPPVYVRKQIVHNAHVVADLEERGAVFVEELEEVPEGATVVFS
                     AHGVSPAVRSDAGERGLDVIDATCPLVAKVHGEARRFAEGGYTIVLIGHEGHEEVEGT
                     VGEAPDSTVLVESIEDVGSLDVRDPDRVAFLTQTTLAVDETKGIVDALRERFPALVGP
                     RSDDICYATQNRQDAVRALARDSDLVLVIGSRNSSNSQRLVEVAERAGCRARLIDDER
                     DIDPAWLAGVETVGLTAGASAPERLVQQVVTALAGLGPVQLEERSVRNEGIRFKLPPE
                     IRKRG"
     gene            2113..3117
                     /gene="hpnH"
                     /locus_tag="VF752_08045"
     CDS             2113..3117
                     /gene="hpnH"
                     /locus_tag="VF752_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX8855539.1"
                     /translation="MPVSMRQSAKVATYLMRQKLARRDKFPLIVELEPLFQCNLACAG
                     CGKIQQPDHVLKQRMSVEQAVGAIEECGAPMVSIAGGEPLIHPEIHVIVDELVKRKKF
                     VYLCTNALLLEKKIDNFKPSPYFSWAVHIDGLKDRHDESVCRDGVFDKAVHAIEVAKE
                     KGFRVTTNTTFFTHDTPENVRAVLDFLNDELEVDLMMISPAYAYERAPDQDHFLGVKE
                     TRELFSKAFADGKRKKWRLNHSPLFLDFLEGKVDFDCTAWGIPSYSLFGWQRPCYLMA
                     DGYAKTYQELIETTDWSKYGRGRDPRCDNCMAHCGYEPTAVLKTTGSLKESLRALVAR
                     "
     gene            complement(3118..>3749)
                     /locus_tag="VF752_08050"
     CDS             complement(3118..>3749)
                     /locus_tag="VF752_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX8855540.1"
                     /translation="EWALLAPAACICAIAMLLGNRDGVWPSLLWPWALAVPAGFALGL
                     FASAPGTRRRILRWFGNPGWLGHLLEGVATLHTLLRHARQFSRAWVGTTIYWAADIAA
                     LYGGLRMFGVHPTLGMLIIGYATGYAATRRSLPLAGAGVTEALMTFSLHWVGVPIVQA
                     LCAVVAYRVFNFLLVLPPALAARGRVQPLIDAARDGRALTARERSLAFD"
BASE COUNT          570 a         1299 c         1358 g          522 t
ORIGIN      
        1 tggccgtgcc gaacatgcgc gcgggagcgc tgcttccaca cggagcgatc cacgatccgg
       61 gccggctcga ccgcttgggg atctctccga tcgtgaacct gcatgttgtg tatgaccgcc
      121 ctgtgctgca gcatgcattt gtggctggcg tccggtcacc agttcagtac gtcttcgatc
      181 gcacgcggga gggcgagttc ggcggcggcc agtacctggc cgtctcgctg agcggcgccg
      241 ccgcggagat ggagcggccg cccgaggagc tgcgccgcca gtacgtgccg gcgctcgcgg
      301 agatcctccc cgccgcacgc aacgccaagg tggagcgctt cttcgccaac cgcgagcacg
      361 ctgccacgtt ccgcgcggcg cccgggcagc tcgcgctgcg gccgccttcg cgcacgcgca
      421 tcccaggcct cgcactggcc ggcgcgtgga ccgccacggg ctggcccgcg acgatggagg
      481 gcgcagtgcg cagcggccac acggcggcgc gggtggcgct cgaggctctc gacagcccgc
      541 ggcccgcacc agcgggtgtg gcggcatgat cggactgcgt ccggagccgc gcgccgccga
      601 gcatgagggg ctgctcgtgc tcgctccgtt gtcgctcgag gcgcgcgccg tgcgctccgg
      661 ggtgggccgc ggccgcgtgc tgcgaacggg catgggcccc gagcgggcgg tcgaagctgt
      721 gaagacgccg gtcgcgcggg aggcggacgc cgtggcggtc gccggcttct gcggctcgct
      781 cgacgctggg ctcgagccgg gcgacgtggt cgtggcgacc gaggtgcgag gccccgacgg
      841 agctgtcgag tgcccgtcgg ccggggttct cgccggagtg ctcgagcgcc acggcgtccg
      901 tgccgtgtgc ggcccgatcg tctcgaccga ccacgtggtg cagggcgacg agcgccgcga
      961 actcgcggcg agcggcgccc tcgcggtcga catggaatcc gcgcaactcg ccgcgggcgc
     1021 tcgcgaccgc ccgatcgccg tgctgcgcgt ggtggtcgac acgccggatc gcgagctgcg
     1081 cgacccgctc gcgaccgtga ctggcggcct gcgcgcgtac cgcgctcttg cgcgggcgtc
     1141 gaaggcgctc ggcgagtggg ccgcggccgt cggcccgcgc cgcctgatgc tcgccagccc
     1201 gcgcgcctcg tgcgcgggtg tcgagcgagc gatcgaggtt gtcgagcgtg cgctcgagca
     1261 gcacgggccg ccggtgtacg tgcgcaagca gatcgtgcac aacgcgcacg tggtcgcgga
     1321 tctcgaggaa cgcggcgccg tcttcgtcga ggagctcgag gaggtgcctg agggtgcgac
     1381 cgtggtgttc tcggcgcacg gcgtctcacc cgccgtccgt agcgacgccg gcgagcgcgg
     1441 cctcgacgtg atcgatgcga cctgcccgct ggtcgcgaag gtgcacggcg aggcgcgccg
     1501 cttcgccgaa ggcggctaca cgatcgtgct gatcggtcac gagggccacg aggaggtgga
     1561 gggcaccgtc ggcgaggctc ccgactcgac cgtgctcgtc gagtcgatcg aggacgtcgg
     1621 ctcgctcgat gtgcgcgacc cagaccgcgt cgccttcctc acgcaaacga cgctcgccgt
     1681 ggacgagacg aagggcatcg tggacgcgct gcgcgagcgc ttccccgcgc tggtcgggcc
     1741 ccgctccgac gacatctgct acgcgaccca gaaccgccag gacgccgtcc gcgcgctcgc
     1801 gcgggactcc gacctcgtgc tcgtgatcgg ctcgcgcaac tcgtcgaact cgcagcggct
     1861 cgtggaggtg gccgagcggg cgggctgccg ggcgcgcctg atcgacgacg agcgcgacat
     1921 cgaccccgcg tggctcgccg gcgtagagac cgtcggactc acagccggcg catccgcgcc
     1981 cgagcgcctc gtgcagcagg tagtgaccgc gcttgcgggc ctcggtcccg tacaactaga
     2041 ggagcgcagc gtccgcaacg agggcattcg cttcaaactc ccccctgaga tccgaaagag
     2101 aggctgactt cgatgccggt ttccatgcgc cagagcgcca aggtcgcgac ctacttgatg
     2161 cgccagaagc tcgcgcggcg cgacaagttc ccgctcatcg tcgagctcga gccgctcttc
     2221 cagtgcaacc tcgcttgtgc cggctgcggg aagatccagc agcccgacca cgtgctcaag
     2281 cagcgcatgt cggtggagca ggcggtcggt gcgatcgagg agtgcggcgc gcctatggtg
     2341 tcgatcgccg gcggagagcc cctcatccac ccggagatcc acgtgatcgt ggacgagctc
     2401 gtgaagcgca agaagttcgt ctacctgtgc acgaacgcgc tgctgctcga gaagaagatc
     2461 gacaacttca agccttcgcc gtacttctcg tgggccgtgc acatcgacgg gctgaaggac
     2521 cgccacgacg agtcggtctg ccgcgacggc gtcttcgaca aggcggttca cgccatcgag
     2581 gtcgcgaagg agaagggctt ccgggtgacc acgaacacca ccttcttcac tcacgacacg
     2641 cccgagaacg tgcgagcggt gctcgacttc ttgaacgacg agctcgaggt ggacctgatg
     2701 atgatctcgc cggcctacgc ctacgagcgc gcgccggacc aggatcactt cctcggcgtg
     2761 aaggagaccc gcgagctctt ctccaaggcg ttcgccgacg gcaagcgcaa gaagtggcgg
     2821 ctcaaccact cgccgctctt cctcgacttc ctggagggca aggtcgattt cgactgcacg
     2881 gcgtggggca tccccagcta ctcactgttc ggctggcagc gcccctgcta cctgatggcc
     2941 gacggctacg cgaagaccta ccaggagctg atcgagacga ccgactggtc gaagtacggc
     3001 cgcggccggg atccgcgttg cgacaactgc atggcgcact gcggctacga gccgaccgcg
     3061 gtgctcaaga cgactgggtc gctgaaggaa tcgctgcgcg cgctggtcgc gcgctgatca
     3121 gtcgaaggcg aggctgcgct cgcgcgcagt gagggcgcga ccgtcgcgag cggcgtcgat
     3181 cagcggctgc acgcgcccgc gcgcggcgag cgctggcggc agcacgagca ggaagttgaa
     3241 cacgcgatag gcgacgacgg cgcagagcgc ctgcacgatc ggaacgccga cccagtgcag
     3301 tgagaaggtc atgagcgcct cggtcacgcc ggcgccggcg agcggaagcg accgccgcgt
     3361 ggcggcgtag cccgtggcat agccgatgat cagcatcccg agcgtggggt ggacgccgaa
     3421 catgcgcagg ccgccgtaga gggcggcgat gtcggccgcc cagtagatcg tggtgccgac
     3481 ccaagcccgc gagaactgcc tcgcgtgccg gaggagcgtg tgcagcgtgg cgactccctc
     3541 gagcaggtgg ccgagccagc ccgggttccc gaaccacctg aggatgcgcc tccgcgtgcc
     3601 cggcgcggaa gcaaacaggc cgagcgcgaa ccccgccggt acggcgagcg cccacggcca
     3661 cagcagcgac ggccagacgc cgtcgcggtt gccgagcagc atcgcgatcg cgcagatgca
     3721 cgcggccggc gcgagcagcg cccactcca
//
LOCUS       DASVXS010000147         5990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_727031, whole genome shotgun sequence.
ACCESSION   DASVXS010000147 DASVXS010000000
VERSION     DASVXS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727031"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VF752_08055"
     CDS             <1..1259
                     /locus_tag="VF752_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEX8855541.1"
                     /translation="AADARRYLDAAILLGDLLCGAAYWDEPGERCSWLGRTLDLAAIA
                     QGGTTPRPMTTSLGPDLYHGQAGVALFLAELFGQTADERFRRTALGAAAGATALLRDA
                     VAAGEAPGAGLYTGASGVAYAIARVASVTACAATAGELGEIGDLLIGSLAVEPVSIPS
                     GPDPNDVVNGRAGTILTLLALAGTPVFRDREEPIVGMAIEVGRAMVGSLGPPRLTGFA
                     HGAAGFGSTLLRLSARTGHAEFRDAGREILAWEDGLFDDRKRQWPDLRDRLPRAPARR
                     RGPDAVTWCNGAAGIAISRLHAMTADPSRRDHYADRAAVALELTREALSVQLGVEDDA
                     SLCHGTSGLVETLLLGSAATHDPELAAAARAATDTLLDGGAWARQSTQDRVRAQPSLM
                     LGAAGVGHQLLRLHEPEVVPSVLGGP"
     gene            complement(2379..3518)
                     /locus_tag="VF752_08060"
     CDS             complement(2379..3518)
                     /locus_tag="VF752_08060"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX8855542.1"
                     /translation="MTTLRRDPSSGREQHAAALELFYDLVFVFAVTQVSHLLLTHLSW
                     EGAGQAALVLLVVWWAWNYTTWVTNELNPESPVVILLLVALMLASLLMAVAIPEAFGS
                     RALLFAGSYVAIQVGRHAFLTFAAAVPGTLERVRAGRILTWFVAAGALWIAGAIADGT
                     ARTLLWLAALALDYTAPLVTYWVPGRKRVTPEDWTVQTSHFAERFQLFVIIALGESIV
                     LIGATTSDLHLDTATLTAFALAFLGAAELWWLYFGSVARIAERILATAPNRTLVARDA
                     YTYLHVLIVAGVIVSAVGDELVIAHPTETLTSAQVIAVVAGPVIYLLAHVLVRLRLVG
                     TLTWKRLTGALACAAVAAFGSFAPALVLGALLGVVLAAVIAWERA"
     gene            3665..4843
                     /locus_tag="VF752_08065"
     CDS             3665..4843
                     /locus_tag="VF752_08065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8855543.1"
                     /translation="MSTYDFIVVGARAAGSSLATLLARSGAKVGVIDRDRFPRDTLST
                     HTVFPNGVARLDQLGVLDRLLERHELAFMEHRSWVLGRQVAGRYPAVNGHDGAIAPRR
                     VVLDAALVDAAREAGAELRLGNAVAKLIGTGTEDDPVSGVVLENGERVYARRVLGADG
                     RASTVARLLGLKKEREMSGECSLSFAYWRGLRSDWYVTLGATEDAMFTRVECEDGIHM
                     ILAGGDSEHTHGSRLERERKYFDVLSRFPDLLDMVELERATLVSELRYAPESLMRGYF
                     RRPSGPGWALLGDAAHFKHPASAQGIADALAHAFHLAGALTRSDPELRRYRLWLDDRA
                     AGHYEWSFKLGSFPREETAGPVFDRLERDPAAARDYLGTFTRHVNPGELSARRTQLAA
                     "
     gene            4956..5342
                     /locus_tag="VF752_08070"
     CDS             4956..5342
                     /locus_tag="VF752_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8855544.1"
                     /translation="MPQYMLLIYTPADARPSPEEMQAEMPKWGEYTEALRSSGAMSAG
                     DALHGTETATTVRVRDGETVVSDGPFAETKEVLGGYYVIDVRDLDEALKWAAKIPSAP
                     RGSVEVRPVMVFDEQGRPVSEAGARA"
     gene            5339..>5990
                     /locus_tag="VF752_08075"
     CDS             5339..>5990
                     /locus_tag="VF752_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8855545.1"
                     /translation="MSSQSTAAAEAAFRRERAAVLATLIRQLGDFQLAEDALQDAFAA
                     AVETWARDGVPDKPAAWITVTARRRAIDRLRRDRVLADRIERLSVLVGREAAAEPEEG
                     SAIVDDRLRLMFTCCHPALAMETRVALTLRLVGGLTVREIARAFLVHDAAMEQRITRA
                     KAKIKAARIPYRVPSAEDLPARVSGVLAVLFLIFNEGYLATGPDTDPVRHELTNEAI"
BASE COUNT          919 a         2142 c         2109 g          820 t
ORIGIN      
        1 acgcggccga cgcccggcgc tacctggacg cggcgatcct cctgggcgat ttgctgtgcg
       61 gcgccgccta ttgggacgag cccggcgagc gatgctcctg gctcggtcgg accctggacc
      121 tcgcggccat cgcccagggc ggcaccactc cccggcccat gacgaccagc ctcggccccg
      181 acctctacca cggccaagct ggcgtcgcgc tgttcctggc cgagctgttc ggacagaccg
      241 cggacgaacg gttccgtcgc accgccctcg gcgccgccgc cggggcgacg gcactgctca
      301 gagacgccgt ggcggcgggc gaggctcccg gcgccggcct ctacaccggc gcaagcggcg
      361 tcgcgtacgc catcgcccgc gtagcgtcgg tgaccgcatg cgcggcgacc gccggggagc
      421 tcggcgagat cggcgacctg ctgatcggat cgctcgccgt cgagccggtc tccatcccct
      481 ccggtcccga cccgaacgac gtcgtcaacg gccgggcggg cacgatcctg accctgctcg
      541 ccctcgccgg caccccggtc ttccgcgacc gggaggagcc gatcgtcggg atggccatcg
      601 aggtcggacg agcgatggtc gggtcgctcg gaccgccgcg gctgaccggc tttgcccacg
      661 gcgcggccgg cttcgggtcg accttgctac gactctccgc gcgcaccggt cacgccgagt
      721 tccgcgacgc cggacgggag atcctagctt gggaggacgg cctcttcgac gaccgcaaac
      781 ggcagtggcc cgacctgcgc gaccggttgc cgcgggcccc ggcccggcga cgcgggccgg
      841 acgccgtcac gtggtgcaac ggcgcagctg gcatcgccat ctcgcgtctg cacgccatga
      901 cggccgatcc gtcgcgccgc gaccactacg ccgaccgggc cgcggtggcc ctcgagctga
      961 ctcgcgaggc gctgtcggtc cagctcgggg tggaggacga cgcgtcgctg tgccacggga
     1021 cgagtggcct agtagagacg ctgctgctgg gcagcgccgc cacgcacgac ccggagctcg
     1081 ccgccgcggc ccgcgccgcg accgacacgc tgctggacgg gggagcctgg gcgagacaat
     1141 cgacccagga ccgagtcagg gcgcagccca gcctgatgct gggcgcggcc ggcgtcgggc
     1201 accagctgct gcggcttcac gagcccgagg tcgtaccctc cgtcctcggc ggcccatagg
     1261 agcccgcgcc tccctcagcc gcgaggcgcg gcggagatct tctcacagcc gacgcgggga
     1321 ctcgaacccc ggaccccttc atacgagtgt tggatcacgg acggaggagg ctcgaggtac
     1381 aggccgcaca cctcggcggc gcgctggtcc aagtccgggc cgagctagga catcacccac
     1441 cgctccaaaa gggcgccctc ggaggcgcgg cgtagcgtcg cccgcgccgg caaggagccg
     1501 cggagagcac aggcgcgggc aggccgtcgc gcgcatggag ctcgactccg atctgttatc
     1561 tggccgcccc ttcgacttgt ccggttgcgc tgcgagcacg gatccgtagg tgtcgtgttg
     1621 cccgacctcc ccgccgtcgt ctggtgagcg ggggaggtga cacgaggcgc acgttcgccc
     1681 tggacgtcta caagcgcttc gccgagatcg ccctggtcga gaaacgggga gccggctcag
     1741 cgctggggtc ggatcgccac caccccgcac gagcgggcct tcgccgagca gctcgcctga
     1801 ggacgaggtg gtgatcgagt ccacctcgat cacctgggcc atcgtcgacc tcatggcccg
     1861 gcacgccggc cgcgtggtgg tctccaagcc acccggccgc ttccctgcgc cagttgcggt
     1921 cacatcaaag cgcccgctgg acaagagtgg ttgtagccac ctgtgctggt gcagcgaatc
     1981 gcagccagct ccgatgctcc agcgcgggct cccgtttggc gactggacgc tcgccgcgga
     2041 tcgacttggc cacatttggc gacgaacggc gacaaccgca agcgcggtac ctggcgagtc
     2101 ccactcgtgg ttcgcgtgga gcgagtgtct cggggagcgg ctcgcagacg ctcgcaatgg
     2161 acgagactcg aaagctgagg ccgccacgtc gcggctcctg cattcgttgc ggaacgtgca
     2221 tctcgacggc gtggcgccgc gccctgagat gcgcggaaag caggattacc aagcggccga
     2281 tcccggatgg agagaaggaa gccgacgggc accgaattcg gcggtttgtg gcagggggga
     2341 caaaatccgg cccctccccc ctaacccgtg aacatggact aagcgcgctc ccaggcaata
     2401 acggcagcga gcacaacccc gagcaacgcg ccgagcacga gggcgggagc aaacgatccg
     2461 aatgcagcca ccgccgcgca ggcgagcgcg ccggtcaggc gcttccaggt gagcgtgccc
     2521 acgagtcgca ggcgcacgag cacgtgggcg agcaggtaga tgaccgggcc ggccaccacc
     2581 gcgatcacct gcgcgctcgt gagcgtctcg gtcggatggg cgatcacgag ctcgtcgccg
     2641 accgcggaga cgatcacgcc ggcaacgatc agaacgtgca ggtatgtgta ggcgtcgcgc
     2701 gccacgaggg tgcggttcgg ggcggtggcc aggatgcgct cggcgattcg cgcgaccgat
     2761 ccgaaataca gccaccacag ctcggcggcc cccaggaagg cgagcgcgaa cgccgtgagc
     2821 gtggcggtgt cgaggtgcag gtcggacgtc gtggcaccga tcagcacgat cgactcgccg
     2881 agcgcgatga tcacgaacag ctggaagcgc tcggcgaagt gcgaggtctg cacggtccag
     2941 tcctccggcg tgactcgttt gcgccccggc acccagtagg tcacgagcgg tgccgtgtag
     3001 tcgagcgcga gcgccgcgag ccacagcagc gtgcgcgcgg tgccatccgc gatcgcgccg
     3061 gcgatccaca gcgcccccgc cgcgacgaac caggtcagga tgcgtccggc gcgaacgcgc
     3121 tcgagggtcc ccggcacggc cgctgcgaac gtgaggaacg catggcgccc gacctggatc
     3181 gccacgtacg agcccgcgaa caacagggcg cggctgccga acgcctccgg tatggcgacc
     3241 gccatcagca ggctcgccag catcagggcg accagcagca ggatcacgac aggcgactct
     3301 ggattcagct cgttggtgac ccaggtcgtg tagttccacg cccaccacac gacgagcaga
     3361 acgagcgccg cctggcccgc gccctcccag ctcagatgcg ttagcagcag gtgtgacacc
     3421 tgcgtgaccg cgaacacgaa gaccaggtcg tagaacagct cgagcgctgc ggcgtgctgc
     3481 tcgcgaccgc tgctcggatc gcgccgaagc gtggtcatcg tgtcctcgcg cattcgcacc
     3541 agctgaaggc ggacagtaac ccggatgtgg ccgccgagga cagggggtta ggcgtgaacc
     3601 gcccgctcga acgccagcta cccccagtgg aaccgcgcgc ccgccccctt acgctggcgc
     3661 gagcatgagt acgtacgact tcatcgttgt tggggcccgc gccgcagggt cttcactggc
     3721 gacgctgctc gcgcgcagcg gcgccaaggt cggcgtgatc gaccgcgacc gcttcccgcg
     3781 cgacacgctc tccacgcaca cggtcttccc gaacggcgtg gcgagactgg accagctcgg
     3841 cgtgctcgac cgcctcctcg agcgccacga gctcgcgttc atggagcacc gctcctgggt
     3901 gctcggccgc caggttgccg ggcgctatcc agcggtgaat ggccacgacg gcgcgatcgc
     3961 gccgcggcgc gttgttctcg acgctgcgct cgtcgacgcg gcgcgcgagg ccggagcgga
     4021 gcttcgcctg ggcaacgcgg tggcgaagct gatcggcacc ggcactgagg acgacccggt
     4081 gtcgggcgtc gtgctagaga acggcgagcg ggtctacgcg cgccgggtgc tcggcgcgga
     4141 cgggcgggct tccaccgtcg cccggctgct cgggctgaag aaggagcgcg agatgagcgg
     4201 cgagtgctcg ctctcgttcg cctactggcg cggccttcgc agcgactggt acgtgactct
     4261 cggcgcgacc gaggacgcga tgttcacgcg cgtggagtgc gaggacggca tccacatgat
     4321 cctggccggc ggcgactccg agcacacgca cggcagccgt ttggagcgcg agcgcaagta
     4381 cttcgacgtg ctgtcgcgct tcccggacct gctcgacatg gtcgagctcg agcgcgcgac
     4441 gctcgtgtca gagctgcggt acgcgcccga gtccctgatg cgcggctact tcaggcgtcc
     4501 gagcgggcct ggctgggcac tgctcggcga cgcggcgcac ttcaagcacc cggcgagcgc
     4561 gcagggcatc gccgacgcgc tcgcccacgc cttccacctc gctggggcgc tcacgcgctc
     4621 cgatccggag ctcaggcgct accggctctg gctcgacgac cgcgctgccg gccattacga
     4681 gtggtcgttc aagctcggca gcttcccgcg cgaggagacg gccggcccgg tgttcgacag
     4741 gctcgagcga gatccggccg cagcgcgcga ctacctgggg accttcactc gccacgtgaa
     4801 cccgggcgag ctctcggcgc ggcgcacgca gctcgctgcc tagtgcctac gcgccagctt
     4861 gccgtcgtgg accgggtact gtcgaattgg cggagcgccg tccgtcgagt gatcggagcc
     4921 ggaatcctcc ggctcgctgc ccaggaggtc acgcaatgcc gcagtacatg cttctcatct
     4981 acacgccggc cgacgcgcgt ccctctccgg aggagatgca ggccgaaatg cccaagtggg
     5041 gcgagtacac cgaagcgctc cgctcctccg gtgcaatgtc cgccggcgac gcgctgcacg
     5101 gcaccgagac ggcgacgacc gtgcgggtcc gcgacggcga gaccgtcgtg agcgacggcc
     5161 ccttcgccga gaccaaggaa gtgctcggcg gctactacgt gatcgacgtc cgggatctcg
     5221 acgaggcgct caagtgggcc gcgaagatcc cgagcgcgcc gcgcggctcg gtggaggtgc
     5281 gcccggtgat ggtgttcgac gagcagggac gacccgtcag cgaggccggc gcacgcgcgt
     5341 gagctctcag agcaccgcgg cggccgaggc ggcgttccgc cgcgagcgcg ccgcggtgct
     5401 cgccacgctc atccgccagc tcggcgactt ccagctggcg gaggacgcgc tccaggacgc
     5461 gttcgccgct gctgttgaga cgtgggcgcg cgacggcgtg ccggacaagc ccgccgcgtg
     5521 gatcacggtc acggcgcggc gccgggccat cgatcgactg cgccgtgacc gcgtactcgc
     5581 ggatcgcatc gagcggctga gcgtgctcgt cggccgcgag gccgccgccg agcccgagga
     5641 ggggtcagcc atcgtggacg accgcctgcg cctgatgttc acctgctgcc accccgcgct
     5701 cgcgatggag acccgcgtgg cgctgaccct gcgtctggtc ggcggcctga ccgtccgcga
     5761 gatcgcccgc gccttcctgg tgcatgacgc cgccatggag cagcggatca cccgcgcgaa
     5821 ggccaagatc aaggccgccc gcatcccgta tcgggtgccg tccgccgagg atctgccggc
     5881 ccgcgtctca ggggtgctgg ccgtcctctt cctgatcttc aacgagggct acctggcgac
     5941 cggccccgac accgatccgg tacgccatga gctgaccaac gaggccatcc
//
LOCUS       DASVXS010000148         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_39881, whole genome shotgun sequence.
ACCESSION   DASVXS010000148 DASVXS010000000
VERSION     DASVXS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39881"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VF752_08080"
     CDS             complement(<1..1306)
                     /locus_tag="VF752_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855546.1"
                     /translation="MQQLQTKTETGGTSALLSRFAGTDTGRAAGMAAAMIATNVIALA
                     FTIVFARLLGASGYGSLAALVSTFLILAVPGSAVQIQVAREIGRELAQGRDAPAAAVR
                     HWTERLLVATAAAAVVGVLLREPLASLIGVEEVPWAAAATLPSAALWVVVCVQRGALQ
                     AFQRYRLVGSSLVGEASGRLAIGVILEAIGLGVTGAFLGTAASLAVVALVLGAELRRQ
                     LADANSQRAFDPGARLRKLLRRAEVPLLALAMIAVLQNIDLIVVKHNANGDPAGSYAA
                     AAVAAKVPIWVAIGLGMYLLPEAARRVSVGMDARPVLARTLAVIAAIALPMVLFYTVA
                     GEPLLRTVFGDDLTLASDALPLLGLAMSLLAATFLAVQYLLALHRQRFLWLLAAAAAL
                     DPVLVNAMGSNLTDVALVLMCLQAALAAAMLVTAVRAKTDERP"
     gene            1386..>2249
                     /locus_tag="VF752_08085"
     CDS             1386..>2249
                     /locus_tag="VF752_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8855547.1"
                     /translation="MQEHALTERLITYDTSTLEGIQSAAGFVKGWLEARDVEVTSATH
                     NGRPVIAATVGAAPEPGVATVVLHGHLDVVPGHSEQFVSRVEGDRLFGRGAYDMKGGL
                     ASMMCATRDLLSQREVRVHFVCVSDEETDEAVQRGSDYLVEQGYTGDFAITGEPTDLH
                     IGVQAKGVLAMRIEVCGKSAHGSTPWLGDNAVLKAIDVFKRIESMPFARESSDLFDRP
                     SISLGRIVGGDALNKVPDLCAIDIDLRYLPNQDPQEILAQVREIPDVSVATLFQRDPA
                     IVRRDNPYVQML"
BASE COUNT          369 a          799 c          779 g          302 t
ORIGIN      
        1 gaggccgctc gtccgtctta gcgcggacgg ctgtgacgag catggcggcc gccagcgcgg
       61 cttgcaggca catgagtacg agcgccacgt cggtgaggtt cgagcccatc gcgttcacga
      121 gcacgggatc gagcgccgcc gctgccgcca gcagccagag gaagcgctgc cggtggagcg
      181 cgagcagata ctgcacggcc aggaacgtgg cggcgagcag gctcatcgcg agcccgagca
      241 gcggcagcgc atccgacgcg agcgtgaggt cgtccccgaa caccgtgcgc agcagcggct
      301 cgccggcgac ggtgtagaag agcaccatcg gcagagcgat cgcggcgatc acggccagcg
      361 tgcgcgccaa taccggccgc gcgtccatgc ccacgctcac gcgccgcgcc gcctcgggca
      421 gcaggtacat gccgagtccg atcgcgaccc agatcggcac cttcgccgcc accgctgcgg
      481 cagcgtagga gcccgcgggg tcgccgtttg cgttgtgctt caccacgatc aggtcgatgt
      541 tctgcagcac cgcgatcatc gccagcgcga gcaggggaac ctcggcccgc cgcagcagct
      601 tgcgcaggcg cgcgcccggg tcgaacgcgc gctgggagtt cgcatcggcg agctggcggc
      661 gcagctctgc acccagcacc agcgccacga ctgcgagcga cgccgcggtg ccgagaaacg
      721 cgccggtcac gccgagcccg atcgcctcga ggatcacgcc gatcgccagc cgcccgctcg
      781 cctcgcctac gagactcgag cccaccaggc ggtagcgctg gaacgcctgg agcgcgccgc
      841 gctgcacgca caccaccacc caaagcgccg ccgacggcag cgtcgcggcg gcggcccatg
      901 gcacctcctc cacgccgatc agcgaggcga gcggctcgcg cagcagcacg cctacgacag
      961 ccgccgcggc cgtcgcgacg agaagacgct cggtccagtg gcgcacggct gcggcgggcg
     1021 cgtcgcggcc ctgcgcgagc tcccgaccga tctcgcgcgc gacctgtatc tgcacggcgg
     1081 agccgggcac ggcgaggatc aggaaggtcg agaccagcgc ggcgagcgag ccgtagccgc
     1141 tagcgccgag caatcgcgcg aagacgatcg tgaacgcgag cgcgatcacg ttcgtcgcga
     1201 tcatcgccgc ggccatgcct gccgcccggc cggtgtcggt gccggcgaag cggctcagca
     1261 gcgcggaagt gccgcccgtc tcggtcttcg tctgaagctg ctgcattggg cggtgtcccc
     1321 agggccactg taggggacct ccgggccgac gcgatgcctc tgagagcccc gttatcctca
     1381 ctgcgttgca ggagcacgct ctcactgagc ggctgatcac ctacgacacc agcaccctcg
     1441 agggcattca gagcgcggcc ggcttcgtga agggctggct cgaggctcgc gacgtggagg
     1501 tgacatcagc cacgcacaac ggccggcccg tgatcgccgc cacggtcggg gctgcgccgg
     1561 aacccggcgt cgctaccgtc gtgcttcacg ggcacctgga cgtggtgccg ggacactcag
     1621 agcagttcgt ttcgcgcgtg gagggcgacc ggttgttcgg ccgcggcgcc tacgacatga
     1681 agggcggcct cgcgtcgatg atgtgcgcga cgcgcgacct gctctcccag cgggaggtgc
     1741 gcgtccactt cgtctgcgtc tccgatgagg agaccgacga ggccgtccag aggggctcgg
     1801 actacctcgt cgaacagggc tatacaggcg acttcgccat caccggcgag cccacggacc
     1861 tgcacatcgg cgtccaggcg aagggcgtgc tcgcgatgcg catcgaggtg tgcggcaagt
     1921 cggcccacgg atcaacgccg tggctcggcg acaacgcggt actcaaggcg atcgacgtgt
     1981 tcaagcggat cgagtccatg cctttcgcgc gcgagtcttc agatctcttc gaccggccct
     2041 cgatctcact cggccggatc gtcggcggcg acgcgctcaa caaggtgccc gacctctgtg
     2101 cgatcgacat cgatctccgc tacctgccga atcaggaccc acaggagatc ctggcgcagg
     2161 tgagggagat ccccgacgtg agcgtcgcga cgctgttcca gcgcgatccg gcgatcgtgc
     2221 ggcgcgacaa tccgtacgtc cagatgctg
//
LOCUS       DASVXS010000149         8567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1223615, whole genome shotgun sequence.
ACCESSION   DASVXS010000149 DASVXS010000000
VERSION     DASVXS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223615"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VF752_08090"
     CDS             <1..772
                     /locus_tag="VF752_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX8855548.1"
                     /translation="SFFEGPVERYGERRNELAGDGASRLSPYLHFGCLSPRELEALLP
                     AGPGAEEYRRQLCWRDFYAHVLAGFPSDARSEHQPRFRGSLSWSHARKPFAAWCEGRT
                     GFPLVDAAMRQLRQEGWMPNRARLVAASFLTKDLWIDWRWGERWFMRLLVDGDEASNN
                     GNWQWIASVGIDPQPASRRILNPTRQQKRFDPEGAYVRRYVPELAAVPDRYLAEPSRM
                     PAALQREVGCVLGRDYPEPIVDHSVARSETLARFRRVG"
     gene            944..1081
                     /locus_tag="VF752_08095"
     CDS             944..1081
                     /locus_tag="VF752_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855549.1"
                     /translation="MAERAGAKITEVEGSHVIMISQPQAVTEVILEALAAVETSVADR
                     S"
     gene            1152..2126
                     /locus_tag="VF752_08100"
     CDS             1152..2126
                     /locus_tag="VF752_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8855550.1"
                     /translation="MKSEPFLGQLSRATRGAGSASLATVLIALAANLLVAVAKSVAAL
                     VTGSASMLAEAAHSWADTGNEVFLLIAFRRSRRPPDRAHPLGHGREAYVWSLVAALGL
                     FVAGAAVSVTHGVQELLRPAPASDFVVGYVVLAVSFVLEGISFLRSVRQARPAADLME
                     RDLIGAVMATSDPTLRAVFAEDGAALIGLAIAAVGLGAHELTGSSTPDAVGSILVGVL
                     LAVVATVLIDRNREFLIGEEADPRVRAAAIRALLAEPDVARVTYLRLEFVGPRVVSVI
                     GDVDLTGDETESNVAVRLRALEAKIDASPAVAGTVLSLSAPDEPSLTE"
     gene            2480..2959
                     /locus_tag="VF752_08105"
     CDS             2480..2959
                     /locus_tag="VF752_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855551.1"
                     /translation="MSDHTPYIDFKNLPAPSEGFLVTHFITVRSVARSRAFYSEVLGG
                     QVVLEENPCMVKLSNSWVIMNPGGPPTPDKPDISVVDYEPGNTVSTFMNLRVADIQAC
                     YEQWSSKGAEFVTPPIDRGAEIRCYMRDPDGYMVEVGQSTGLLEGELAKKRPEDLPG"
     gene            3081..3920
                     /locus_tag="VF752_08110"
     CDS             3081..3920
                     /locus_tag="VF752_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855552.1"
                     /translation="MARHTVGPRRTDSLRAYVRATRIIGGLSVLVLFGAIALDLANDR
                     FWSRHTLFTGLVASLIVVAVTVAALNEVLERRQRERWSVLAQYALLDFVRTARLVWSG
                     LLELAGLVPDGELGDEALAAGSEAVRDTPRLAAAMDEMLASADEREQLHRLIAELLGH
                     GQEVLGRWADVMVNSATYAEIVDRHVELYSRLYWWGSVLDESEPLQEHLSRPRLSRVS
                     PATQASGPVEDEWLRDNLVAIAQLAESLDRVSFELAMRIVPLDWWTAQLPDRPSTIED
                     LRS"
     gene            complement(3990..5600)
                     /locus_tag="VF752_08115"
     CDS             complement(3990..5600)
                     /locus_tag="VF752_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855553.1"
                     /translation="MKPPTESPIDAPPSPTLSASQLATLAGLGEERKAEVGDVLYGVG
                     DRSYPFIAILEGEVAILDAAGNEIVRHGPSGFLGELNMLSGQTVFVTAVVTQPLRYIA
                     VDRDVMRSLLYEDGPLGDVVLSAFIARREGLQRVQGIGLEIVGPHSSNATRRMIEFAR
                     ANRLPFTWRDPERADDRAAAGLVAGLDEASLPLVRLPGGAELQGPSPGQLSRALGIGR
                     ELAPREQVDLLVVGAGPAGLGAAVYGASEGLDTLVVESTGLGGQAGASRRIENYLGFP
                     AGITGNELTSRAVTQARKFNARLATPYRAVALQPGNRRHIVQLEEDREVAAGAVLIAT
                     GAQYRRLPVDHLDEYEGISVFYAAGPPEAQLCGAERVAVVGGGNSAGQAAVWLARGGA
                     LVTLLHRRADLQETMSDYLIRELERYGVAVRDRSEIGALHGADGQLEAVTLKSGERLR
                     FSFLFLFLGAVPCTEWLDDAVARDDHGFILTGASAGAANLLGTTVPGVFAAGDVRSGS
                     TKRCATAVGEGAWAIQLVHTHLAARSTD"
     gene            6057..6818
                     /locus_tag="VF752_08120"
     CDS             6057..6818
                     /locus_tag="VF752_08120"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8855554.1"
                     /translation="MAGSVVRELRGGRADRRRAQTSARGSTSRSRARAQSRRAPQRTR
                     ARGQAKRTSTGARAKAQPKRATTRARAKPQPKRQPQRKPLDDAAIAQKVEATIFQGSG
                     VDGRDIDVEVTDGTVSLRGEVSSRELGRELEARATGVSEVRSVQNQLRVRETPSPEHV
                     DLANRQQEATPPAGVRAYPERTPAPANVEERRPAPAVRTSVPAKPDEQHASAPLPDAA
                     GTRSGSETDSPAERAKPEEVEADNDRTGRPNEPSG"
     gene            7055..8176
                     /locus_tag="VF752_08125"
     CDS             7055..8176
                     /locus_tag="VF752_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8855555.1"
                     /translation="MTAHGLTRRDLVRGGAIGAAAALAGVGLHLALRGAGSPAPVSPP
                     LELIGVDARGTGFAGTVSGQQFTAWGFNWGGPGEYRDLGVVRKRFSEMRALGANTVRI
                     HQQFADLTESPTEANEHELARLASIVEIAEQEGLYLDISGNEVWLPASAPAWYGQLDE
                     ASRWDAQATYWSAVARVCAGSPAVFCYDLLSEPLVGNGVPNGDWYTGKLGDFYFGQRI
                     SLELAGRRPDDVARAWVEHLTAAIRAEDKRHLITVGLLPDMHMSRFDADAVAPLLDFL
                     AVHVYPQTGRAAESVALVERLAALGKPVLIEETYLLAGDVPTLERFVVGTRPYAAGWL
                     GFYFPGPPGGPRSIQAALAQEWLRLFQSYTPTRRRQSDR"
     gene            8232..>8567
                     /locus_tag="VF752_08130"
     CDS             8232..>8567
                     /locus_tag="VF752_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8855556.1"
                     /translation="MTGATGYVGSHAIAALRGAGHRIRVLARSPDRLPDALAPLGVDQ
                     VETVTGDVTDPAVVEGALEGADAVLHAASVFSMDARKADEMRSVNVRGADIVLGAAHR
                     LGLDPIVYVS"
BASE COUNT         1345 a         2946 c         2949 g         1327 t
ORIGIN      
        1 ctcgtttttc gagggaccgg tcgagcgtta cggcgagcgg cgcaacgagc ttgccggaga
       61 cggcgcctcc cggctctcgc cctacctcca tttcggctgc ctctcgccgc gggagctcga
      121 ggcgctgctg cccgccggcc caggtgccga ggagtaccgc cggcaactct gctggcggga
      181 cttctatgcg cacgtgctgg cgggctttcc ttccgacgct cgctccgagc accagcctcg
      241 cttccgcgga agcctgtcgt ggagccacgc acgcaagcca ttcgccgcct ggtgcgaagg
      301 gcggaccggc tttccgctgg tggacgccgc catgcgccag ctgaggcagg agggctggat
      361 gcccaaccgc gcccgcctcg tcgccgcctc ctttctcacc aaagacctct ggatcgactg
      421 gcgctggggc gagcgctggt tcatgcgcct cctcgtggac ggcgacgaag ccagcaacaa
      481 cggcaactgg caatggatcg cctccgtcgg catcgatccc caacccgcct cccgccgcat
      541 cctgaacccg acacgccagc agaagcgctt cgacccagag ggcgcgtatg tgcgccgcta
      601 tgtgcctgag ctggccgcgg tgcctgaccg gtacctggcc gagccatcgc gcatgccggc
      661 ggcgctgcag cgggaggttg ggtgcgtcct tgggcgcgac tatccggagc cgattgtgga
      721 ccactcggtg gcgcggtcgg aaacgcttgc gcgatttcgt agggtcggtt agatcggcca
      781 tagctaaggc attcgcgcaa tcagccgagg tcggccggaa ggaccaggcc ccagcccgga
      841 cattgatcgc gcggcgccac atcgcccgag cgggaacgcg cccgagcagt cggaccccgg
      901 gcgccgcgtg cagcgagggc gccgcgccga cgtcgtccgt tcgatggcgg agcgagcagg
      961 cgcgaagatc accgaggtcg agggctcgca cgtaatcatg atctcgcagc cccaggcggt
     1021 gaccgaagtg atcttggagg cgctcgccgc ggtcgagacc tcggtagcag acagatcctg
     1081 aatgtggtat cccgcgcgcg cacgtgaatc gtggtgcgga cctgccgtga aatcagaggt
     1141 acggacctgc tgtgaagtca gagccgtttc tcggccagct ctcgcgggca acccgtggcg
     1201 ccggctccgc gagcctcgcg acggtcctca tcgccctcgc cgccaacctg ctcgtcgccg
     1261 tcgcgaagtc ggttgcagcg ctggtgaccg ggtcagcgtc gatgctggct gaggcggcgc
     1321 actcctgggc ggatacaggc aacgaggtat ttctgctcat cgcgttccgt cggtcgcgcc
     1381 gcccgcccga ccgcgcgcat ccgctcggcc acggccggga ggcctatgtg tggtcgctgg
     1441 ttgcagcact tggcttgttc gtggcgggcg ccgccgtctc ggtgacgcac ggggtgcagg
     1501 aactcttgcg tcccgcgccg gccagcgact tcgtcgtggg ctatgtcgtg ctggcggtct
     1561 ccttcgtgct cgagggaatc tcgttcttgc gctcggtccg gcaagctcgg cccgcggctg
     1621 acttgatgga gcgggacctg atcggcgcgg tcatggccac gagcgacccg acgcttcggg
     1681 ccgtgttcgc cgaggacggg gccgcgctca tcggcctggc gatcgcagcc gttggactgg
     1741 gagcccacga gctgacgggc tcctcgaccc ccgatgccgt cggatccatt ctcgtcggcg
     1801 tcctgctggc ggtggtcgcg acggttctga tcgaccggaa tcgcgagttc ctgatcgggg
     1861 aagaggccga tccccgggtc cgcgccgccg cgatccgggc gctgctcgcc gagccggacg
     1921 tcgctcgcgt gacctacctg cgcctcgagt tcgtcggccc gcgcgtcgtt tctgtcatcg
     1981 gcgacgtcga cctgaccggt gacgagacgg agtcgaacgt agccgtccgg ctccgcgcgc
     2041 tggaagcgaa gatcgacgcg tctcctgcgg tggccggcac cgtgctcagc ctgtctgcgc
     2101 cggacgagcc atcgctgacc gaatgaacgg ccgcgcccgt gctcacctcg gccttcgcct
     2161 ccacgtcgtc gcccgaaagg aagatcaccc gctgtccgcc ggcgtcgtgc gggttcgatc
     2221 cgagcaccgc ggcgccgagc gtgttcgcag ctttcacgac gcgcgcaccg gcaacgaggt
     2281 ccgccacacc tcgctcgaag tcctcccctt gagatcgttg ggatcgaagt cgtttgtcac
     2341 gtcgatcacg atccgaccat tcattcgaga ccctggacgg cctgcgggac gttgtgccac
     2401 gggacggcga taaccacgat gctggcagcg gatgtaggaa gacgtgcccc ccaagcgcgt
     2461 gcgagaggag cagacaacca tgagcgacca cacgccatac atcgacttca agaacctgcc
     2521 ggcgccgagc gagggcttcc tcgtcacgca cttcatcacc gtacgaagcg tcgctcggtc
     2581 ccgtgcgttc tactccgagg tcctcggcgg ccaggtggtg ctggaggaga acccgtgcat
     2641 ggtcaagctc tcgaattcgt gggtgatcat gaacccgggc ggtccaccca cccccgacaa
     2701 accggacatc tcggtcgtcg actacgagcc cggaaacacg gtgtcgacct tcatgaacct
     2761 gcgagtcgcc gacatccagg cgtgctacga gcagtggagc tcgaaggggg cggagttcgt
     2821 gacgccgccg atcgatcgcg gcgccgagat ccgctgctac atgcgggacc ccgacggata
     2881 catggtcgaa gtcgggcagt ccaccggact gctcgaagga gagcttgcca agaagaggcc
     2941 cgaagacctc ccgggctgag gggcccgccg ccatccgctc gctgttcgtg gctgttgctt
     3001 gcgcggtcgg cgaaacgagt gcgcggtgca cgacacgccg agccccgcac gctcgcgtgc
     3061 cgcaatatgc tcggcaagag atggctcgtc acactgtcgg tccgcgccgc accgactcgc
     3121 ttcgggcata cgtgcgcgcg acgcggatca tcggcggcct cagtgtgctc gtcctattcg
     3181 gggcgatcgc cttggacctc gccaacgaca ggttctggtc gcgtcacacg ctcttcaccg
     3241 gtctcgtcgc gagcttgata gtcgtcgcgg tgaccgtcgc ggccctcaac gaagtgctcg
     3301 agcgccggca gcgcgagcgc tggtcggtac tcgcgcagta cgcactgctc gacttcgtcc
     3361 gcacggctcg cctcgtgtgg agcggtctgc tcgagctcgc cggcctcgtt ccggatggcg
     3421 agctcgggga cgaggcgctc gcagccggct ccgaggcggt gcgcgacact ccgcgtctcg
     3481 ccgcggcgat ggacgaaatg ctcgctagcg ctgacgaacg cgaacagctc caccggctga
     3541 tcgcagagct gctcggtcat ggccaggagg tgctcggacg gtgggccgac gtgatggtga
     3601 actcggcaac atacgcggag atcgttgatc gccacgtcga gctctacagt cgtctgtact
     3661 ggtggggcag cgtactcgac gagtcggagc cgcttcagga gcacctgagc cggccgaggc
     3721 tgagccgtgt cagccccgca actcaggcct cgggcccggt cgaggacgag tggctgcgtg
     3781 acaatctcgt cgccatcgcg cagctcgctg agtcgctcga tcgagtctcg ttcgaactcg
     3841 cgatgcggat cgtcccgctc gactggtgga ccgcgcagct gcctgaccga ccgtcgacga
     3901 ttgaagatct gcggagctag ggccgtctca tcgtgattgg gtcaccgggg gcttccagca
     3961 tgtcggtggc gcggtgacgg cctgagactt cagtcggtgg agcgcgcggc gaggtgggtg
     4021 tggacgagct gtatcgccca ggctccctcg ccgacggcgg tggcgcagcg tttggttgag
     4081 cccgagcgga cgtcgccggc tgcgaacaca cccgggacgg tggtgcccag caggtttgcg
     4141 gcgccggcgc tggcgccggt gagaatgaag ccgtggtcgt cgcgcgcgac cgcgtcatcg
     4201 agccattctg tgcacggcac ggcaccgagg aacaggaaca ggaacgagaa ccgcaggcgc
     4261 tcgccgctct tgagcgtcac cgcctcgagc tgaccatccg caccgtggag cgcgccgatc
     4321 tcgctgcggt cgcgcaccgc cactccgtag cgctcgagct cgcgtatcag gtagtcggac
     4381 atcgtctcct gcaggtcggc ccgccggtgc aggagcgtga ctagcgcgcc gccccgagcg
     4441 agccacacgg cggcctggcc ggccgagttc cctccgccga cgacggccac gcgctcggcc
     4501 ccgcagagct gcgcctcggg cgggccggcg gcgtagaaga cgctgatgcc ctcgtactcg
     4561 tcgaggtgat cgacaggcag gcgccggtac tgcgcgccgg tggcgatcag gaccgctccg
     4621 gcagcgactt cgcgatcctc ctccagttgc acgatgtggc ggcgattgcc gggttggagc
     4681 gcgaccgcac gatatggcgt ggccaggcgc gcgttgaact tgcgcgcctg ggtgacggcg
     4741 cggctggtga gctcgttgcc ggtgatcccc gccgggaagc cgaggtaatt ctcgatcctc
     4801 cgcgacgcac cggcctgccc gcctagtcca gtgctctcga cgacgagcgt gtccaggcct
     4861 tcggacgctc cgtacacagc ggcgccgagg ccggccggac cggcgccgac cacgagcaga
     4921 tcgacctgct cgcgtggcgc gagctcgcgg ccaatcccga gcgcgcgaga aagctggccc
     4981 ggcgaggggc cttggagctc ggcgccgccg ggcagcctga ccagtggcag gctcgcctca
     5041 tcgaggcccg ccaccagacc ggccgcagct cgatcgtccg cgcgctcggg gtcgcgccac
     5101 gtgaacggca ggcggttggc gcgcgcgaac tcgatcatcc gcctcgtggc attcgacgaa
     5161 tgcggcccca cgatctcgag accgatcccc tggacacgct gcagcccctc gcgcctggcg
     5221 atgaacgcag acaacacgac gtcgccgagc gggccgtcct cgtacaacag cgagcgcatc
     5281 acgtcgcggt cgaccgcgat gtaacgaaga ggctgcgtga cgaccgccgt gacgaacacc
     5341 gtctgtcccg agagcatgtt cagctccccg agaaaacctg aaggtccgtg acgaacgatc
     5401 tcgttgcccg ccgcgtcgag gatcgcaacc tcgccctcga ggatcgcgat aaacggataa
     5461 ctccgatcgc cgacgccata cagcacgtcg cctacctctg ccttccgctc ctcacccagc
     5521 ccagcgagag tggccagctg cgacgcggac aacgtcgggc tcggcggggc gtcgatcggg
     5581 ctctcggtcg gcggcttcat cgtgcaaatg gagcgtagcc aggcgcgagc gccggctggg
     5641 caacgcggac ctcatctcgc gcgctggtct ctcgagacaa gttctgcgct tagcagcgcg
     5701 tgctctctcg agcggcgcaa gctcgacaac tgagagtgcg cccggggaga ctcgaagtcc
     5761 cacccgggat ccccccggac aaggccctca accttgtatg gggtatctcc ctggtgcgac
     5821 ggccaggtca gcccaggtgg cgactgcagt cacgctttcc gtcttagaag aagaccacct
     5881 cacgccgtcg gccgcgtttt ccggtcgccg aactgggcac ccccaaggga ccgccgaatc
     5941 tttggacccg ttacgaagga gcacctccga tgtttacgcg cgctatttca acggccgaga
     6001 gcgctatgtc gtccgcggcg agactaatgc tggcgcctgc gaggctgacg gggcgtgtgg
     6061 ccgggtcggt ggtgcgtgag ctgcgcggcg ggagggcgga caggcgacgg gcgcagacat
     6121 ccgctcgcgg ttcaacctcc cgtagccgcg cgagggcaca atcccggcga gcgcctcaac
     6181 gcacccgcgc gaggggccag gcgaagcgca cgagcacggg cgctcgagca aaggcgcagc
     6241 ccaagcgcgc aaccacccgc gccagggcaa agccgcagcc aaagcgccag cctcaacgca
     6301 aaccgcttga cgacgcggcg atcgcgcaga aggtcgaggc gaccatcttc cagggcagtg
     6361 gcgttgacgg tcgcgacatc gacgtcgaag tcaccgacgg cacggtctcg ctgcgcgggg
     6421 aagtcagcag ccgggagctg ggtcgagaac tcgaagcgcg cgcgacgggg gtatccgagg
     6481 tgcgcagcgt gcagaaccaa ctgcgcgttc gcgagacccc atctccggag cacgttgact
     6541 tggccaaccg ccagcaggag gcgacgcccc cggccggggt ccgcgcatat cccgagcgca
     6601 cgccagcccc ggccaatgtc gaggagcgca ggccagcgcc cgctgtccgc acgagcgtgc
     6661 cggcaaaacc cgacgaacag cacgcatctg cacccctccc agatgccgcc gggacgcgct
     6721 ccggctcgga aactgactcg ccagccgagc gggccaagcc ggaagaggtc gaggcggaca
     6781 acgatcgcac gggccggcca aacgagccaa gcggctagtc ctggactgcg agttcgacga
     6841 gcgccaaacg ccgccatcat tggcgttcac cgccccgtgg cgcccggccc aagtgacgta
     6901 acaacggccg acgggcggct acggctgatc aatggtctct cgcagagccg atcggcggct
     6961 gagccggagc tggcaccaca gattgccgcc ggaacgccgg tgggggagta cggtgccttc
     7021 atcccgacgc tggaggaatg gagcgtcttc tgcgatgacc gcacatgggc tgacccgccg
     7081 tgacttggtg cgtggaggag cgatcggggc ggcggctgcg ctcgcgggcg tcgggctcca
     7141 tctggccctc cgcggtgcgg gatcgccggc acctgtttcc ccgcccctcg agctgatcgg
     7201 cgtagatgca cgcggtaccg gcttcgcagg cacggtgtcc gggcagcagt tcacggcgtg
     7261 gggcttcaac tggggcggac cgggcgagta ccgcgacctc ggcgtcgtgc gcaagcgctt
     7321 ctccgagatg cgtgcgctcg gcgccaacac ggtccgcatc caccagcagt tcgccgacct
     7381 gacggagtcg cccaccgaag cgaacgagca tgagctggcc cgactcgcga gtatcgtcga
     7441 gatcgccgag caagaggggc tgtacctcga catctccggc aacgaggtgt ggctgccggc
     7501 gagcgctccg gcctggtacg gacagctcga cgaggcctct cgttgggacg cgcaggccac
     7561 ttactggtcg gctgtggcgc gtgtgtgcgc gggcagcccc gcagtcttct gttacgacct
     7621 cctgagcgag ccgctcgtgg gcaacggggt gcctaacgga gactggtaca ccggcaagct
     7681 cggtgacttc tacttcggcc agcgcatatc gcttgagttg gcgggccggc gccccgacga
     7741 cgttgcgaga gcgtgggtcg agcacctgac ggccgcgatc cgcgctgagg acaaacgcca
     7801 tctgatcacc gtggggctgc ttccggacat gcacatgagc cggttcgacg cggacgccgt
     7861 cgcgccgctg ctggacttcc tcgcggtgca cgtctacccg cagaccgggc gggcagccga
     7921 gtcggtcgcg ctggtggagc ggctggcggc gctgggcaag cccgtgctca tcgaggagac
     7981 ctacctgctc gcgggcgacg tgcccacgct cgaacgcttc gtcgtcggta cgcggccgta
     8041 cgccgccgga tggctcgggt tctacttccc cggcccgccg gggggaccgc gcagcatcca
     8101 ggcggcgctg gcccaggaat ggctacggct atttcagagc tacacgccta cccgccggcg
     8161 gcagtcggac cgctgacgga cctccgcttc cgggtcaccg gcgacgcgcg aggggattag
     8221 tggacgtcct cgtgaccgga gcaacgggct acgtcggctc gcacgcgatc gcggcgctcc
     8281 gcggcgccgg gcaccggatc cgcgttctgg cgaggtctcc ggacaggctg ccggacgcgc
     8341 tcgcaccgct tggcgtcgac caggtcgaga ccgtgaccgg cgacgtgacg gatcccgcgg
     8401 tcgtcgaggg cgcactcgag ggagccgacg ccgtgctgca cgccgcctcg gtgttctcca
     8461 tggatgcccg caaggcggac gagatgcggt ccgtgaacgt ccgcggcgcc gacatcgtgc
     8521 tgggtgccgc gcaccgcctc ggcctcgacc cgatcgtcta cgtgtca
//
LOCUS       DASVXS010000150        21922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1173173, whole genome shotgun sequence.
ACCESSION   DASVXS010000150 DASVXS010000000
VERSION     DASVXS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21922
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173173"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VF752_08135"
     CDS             <1..740
                     /locus_tag="VF752_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855557.1"
                     /translation="IARVVLEGRARTLPRPVTAPKRRLGHSLALGTACVALLAFSLSS
                     DDTFPVFARAFDLHPETAVATTRPEVGLLVLVDPSQVPAAARALERRGMRASFVVATM
                     PRPGLMSALQPYGDDAIPQLDSRAFVHWVGAKRELRQVATAVGYGKHYRYVSGDGFSL
                     GEYVLAHSAGGLPVGPAVTITGPATQKLAAGQLVQVRLAGPQSTWAPTLDALASDCQA
                     ARLHTLSARQLLVSSGDRSSDQLAASG"
     gene            745..1431
                     /locus_tag="VF752_08140"
     CDS             745..1431
                     /locus_tag="VF752_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8855558.1"
                     /translation="MRVAALAVGAFAVNSLPGLAPLVAPVADVLGIARRIDRSPGVAL
                     TFDDGPHPDGTPGVLELLRRAGATATFFLAGEQVELRPALAAEIVAAGHGVGLHCHRH
                     RNMLRLTPAQIADDLRRGAATIEDATGHSPRLHRPPYGIYTALGLAAVRTRWRPLLWS
                     RWGRDWRARISPHAIARMVTRDIGPGDVLLLHDADWYSAPGSWTRTLAALPEVLGAIE
                     ARGLKTVPAA"
     gene            1467..1799
                     /locus_tag="VF752_08145"
     CDS             1467..1799
                     /locus_tag="VF752_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX8855559.1"
                     /translation="MGRLDRIPFVELPGGLRMHVARDPISRLFGLAHLDEIPPRSALT
                     LPHCRSVHTYGMRFPLDLIWIGDELRLDREVPPNRVAPGRGGRHVVETRAGEGELFLE
                     GLRLALEH"
     gene            complement(1767..2921)
                     /locus_tag="VF752_08150"
     CDS             complement(1767..2921)
                     /locus_tag="VF752_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855560.1"
                     /translation="MSAPLIAGTEDHPHARLVLGAGLASGSPSHAYLFHGPAGTGKRT
                     AARALAAELLAEGAPDPESVRTRVMHGVHPDLTWVAPTGAHVMRVDDIDEPVVAAATR
                     TPFESSRRVFVLERVETMNDEVANRMLKTLEEPPPFVHLILLTEQLGGVLETVVSRCQ
                     VVRFDPLPPERIAEGLVADGVPADVADSCARVSLGNGARARVFATEEGAALRRDADAL
                     VADALASDDSARAAKEPWRALLERAEERRVAAEEAVERAKKERLELEPKGRERRAIER
                     EFEEAAKRDGRRARTEVLDTGLELAAIGFRDLLAVQTGAGEAVLGRDRIEALQAAASG
                     RVLDPFALREAAEICEEARSSLELNVSEDLALAALSFRLRSVLEREPQAF"
     gene            complement(2918..3532)
                     /gene="tmk"
                     /locus_tag="VF752_08155"
     CDS             complement(2918..3532)
                     /gene="tmk"
                     /locus_tag="VF752_08155"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX8855561.1"
                     /translation="MRGLFVTLEGIDRSGKTTQTQLLVDALGNDQAISVREPGGTAVG
                     ERVRELLKDPGVEIGAEAEALLFAAARAELVAQVIAPALEQGLVVVSDRFLDSSLAYQ
                     GGARGLGLQGVARINEWATGGVVPDLTILLRIDPETAASRAGEADRFEDEGTDLQRAV
                     ADTYAQLAAADPSRWVVVDASRSPDDVHGDVLDAVVRARSRVAV"
     gene            3584..4519
                     /locus_tag="VF752_08160"
     CDS             3584..4519
                     /locus_tag="VF752_08160"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8855562.1"
                     /translation="MRAVVTGGAGFIGSNLVDALLARGDEVTVVDNLSTGRRSNLDQA
                     IAGGAVLAEIDIREADAVRDLVTRVQPEAVFHLAAQIDVRKSVADPANDARINVEGTI
                     NVLNAALAAGVRRFVNTSTGGAIYGEGRVLPAPEDHPVAPEAPYGQSKFAAEGYCSLF
                     NRLHGLSTVSLRYGNVYGPRQDPLGEAGVIAIFSGKLIEGGRPTVFGSGEQTRDYIHV
                     DDVVRANLLAADSDETGAFNIGSCEEVSVLELVDAFREISPDSFDPEFAPERPGEVQH
                     IYLDYSRAREGLGWEPQVTLDEGLRSTVESVRESR"
     gene            4529..4684
                     /locus_tag="VF752_08165"
     CDS             4529..4684
                     /locus_tag="VF752_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855563.1"
                     /translation="MTVVWVAIAVVVLVVLGALVVARTRGITRTDARERMADTAADFR
                     DWIRLGR"
     gene            complement(4686..4856)
                     /locus_tag="VF752_08170"
     CDS             complement(4686..4856)
                     /locus_tag="VF752_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855564.1"
                     /translation="MRREEAEALCVRLCHEHPDRATHAWLPRRLATDSWSVVKVKIPR
                     TRGVHPLLDRRG"
     gene            5000..5599
                     /gene="nrdR"
                     /locus_tag="VF752_08175"
     CDS             5000..5599
                     /gene="nrdR"
                     /locus_tag="VF752_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX8855565.1"
                     /translation="MRCPYCSFESTRVVDSRLTEPGDAIRRRRECASCGQRFTTFERA
                     EGPTLVVQKRDGSQEKFDRQKLVDGMLRAASKRGVDVADLEAIADSIAGDIRRAGGSM
                     SAQEIGERVMRGLVALDRVSALLFAAVYRSFDDLDDVEAEVRRIESQSVAPANQLDLA
                     EPGSVLSDPRASIGPSPSDPSGETREASEIDRRGHAVQP"
     gene            5583..9545
                     /locus_tag="VF752_08180"
     CDS             5583..9545
                     /locus_tag="VF752_08180"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX8855566.1"
                     /translation="MQSSRSAAAPPTQVSTDGVQIGRRFTQAGEHPFDMVEWEIRDAV
                     IGNPESPAFEQHGVEFPASWSQNATNIVSQKYFRGKLGSEQRESSVKQMVGRVAGTIA
                     EWGREGGYFASGDDGDAFEAELTHILLNQYAAFNSPVWFNVGFEERPQCSACQPYHAL
                     ISTPDGLVPIGELVTEDQVGREVYDAEGTTRIVATKANGRKAVWRVQLRNGSYVEATA
                     DHVVRAVGERRSALEWLRVDQLEPGMRMHLHPHRAKVAAPALVTAGGASGVDEPASAD
                     AVAVAEAALAGWLQADGFVGQYDGTNRSLTIEFQVADDAEYEWVMDNLDVVFPDVHRK
                     VREADTKDTRVQRIRLYGEVLRAFVERWDLLARGTEIRVPSRLWTASHDEIAAYLRSI
                     FQADGYISLRHDNGCENARIGFAVIGERWTEDVQLLLGVLGIYSRRTRKLERRPDRHD
                     LHEVSVSIGSERARFAELVGFVSRAKQERLLSSLELRNPKRCPDLREEEIVSIEPLGD
                     MEVFDIQTESGEYLTNYIAVHNCFILSVQDTIESILDWNTKEGMIFRGGSGSGINLSN
                     IRGSQEHLSKGGLASGPVSFMRGADAWAGTIKSGGKTRRAAKMVVLDVDHPDIEAFIW
                     CKAHEEEKAAALRDAGFDMRLDSEAFASIQYQNANNSVRVTDEFMERAERGEDWELKA
                     RVGGETLKTVDARDLLRQISDAAWRCADPGVQYDTTINEWHTSPNSGRINASNPCSEY
                     MHVDDSACNLASLNLMKFRREDGTFDVETFEHCVDVVILAQEIIVGPSSYPTEEIGKN
                     ARAFRQLGLGYANLGALLMSNGLPYDSDEGRAQAAAITALMTGRAYRKSAEVAAVMGP
                     YDRYEENREPHNHVMRKHRDAAYEIDALVAERELVKAARRAWDEAVELGEAHGYRNAQ
                     ATVLAPTGTISFLMDCDTTGIEPDFSLVKFKELVGGGQMKIVNNMVPLALRTLGYSEE
                     QVERIIAYVNERATIVGAPELAPEHLPVFDVAVGERAISHMGHIKMMGAVQPFISGAI
                     SKTVNMPETTTVEDIADAYLQAWKLGVKALAIYRDGSKTAQALSTSGSKKKDKKAEAE
                     EPVADPRPHRRRMPQERQSITHKFRIGQGHEGYITAGMYEDGSLGEIFLTDIGKEGST
                     LRGMMNSFATAISIGLQYGVPLDTYVRKFSYMRFDPEGMTTNPEIPFAKSMPDYIMRW
                     LASRFLDVDVQEELGILTPEVRARKAAHDALMRGDTAAPAAEPETTEAKPANGNGTNG
                     GNTAAKPAASALTDTPPVLPAKLAGLDLGPACEQCGGMMQRTGSCYTCSSCGNNTGCG
                     "
     gene            complement(9630..11066)
                     /locus_tag="VF752_08185"
     CDS             complement(9630..11066)
                     /locus_tag="VF752_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8855567.1"
                     /translation="MPLTAGEPAIKLRIKPRLAAGSCRTKVRAERLDRLSADDARILR
                     LERGTVRGHTCKVILLEPGEHGSLPSTDALREHVSTRLDAAPRLRRCLASTPLHLAPP
                     AWVDDQEFDIARHVRPVETRGAVSPPQLDEIVGRLMEERLDRSHPLWQLDVIDELTDG
                     SMALVWRLHHCLADGTTAVRLGSDVLWSEHPDDVVPASPNWSPDACPGSAGLLARGLR
                     DRARQAAQRVPRRGRRPQDQATSVLRSRAAIRRELARTASRSPLDRRIGSTRSIAFQR
                     APLDDCRRAGKAIHDAVTVNDVVLAIVAGAVREWIAHRHGHDEVVRVKVPVSLHDKAG
                     GDREANRDSYFFVDLPVGEPDPVKRVLAINRETTERKVDRDAEALYRLGLHRPVAHWA
                     MSPRVFTFNVSNVRGPAQPVYVLGARVREMYSLVEVAQRHALRVAVVSACGSIHFGLC
                     ADRDAVPDVAVLADGLERSTAELLECAH"
     gene            11180..11410
                     /locus_tag="VF752_08190"
     CDS             11180..11410
                     /locus_tag="VF752_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855568.1"
                     /translation="MSLHIRRIIAGAIATASLLAFSVPALVHASSATKAHSVSAHAAK
                     RHHRHHSRIPQHNGGDHDADNNGGPSDGDGNI"
     gene            11526..11990
                     /locus_tag="VF752_08195"
     CDS             11526..11990
                     /locus_tag="VF752_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855569.1"
                     /translation="MRPAEVPANWKVGQLPSRKAELAYPPQWRAITTDPGTFSAALLG
                     ANGAIRGYLNATPQSGAETLANWAKFRPAHNGEEGDRSVVREASATGLPFRSATGSCV
                     MDHYTTTSARYREIACIVRGARATTVVVAAAQPGDWARLRPQLERSITSFAS"
     gene            complement(11971..15006)
                     /locus_tag="VF752_08200"
     CDS             complement(11971..15006)
                     /locus_tag="VF752_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8855570.1"
                     /translation="MTTSGPRSEVRKTVTAVFCDVVGSTSLGQRLDPEALRLVMRRYF
                     EEMRGVLEAHGGLVAKFIGDAVVGVFGVPRLHEDDALRAVRAADEMRNRLAELNRSLR
                     AQFDVELEAHLGVCTGEVVLGADDEVVLGDVMNTAARLEQIAAPDEILIADDTHRLVR
                     HAVTTEPVAGMELKGMRSRVTAWRLLAIDPTAEPLTREFDRPLVGRERELRLLRRAFE
                     RAVAERRCVLATVLGFPGIGKSRLALELAASVAGEAQVLVGHCRSYGEGITYWPVAEM
                     VREAAGVNTYSGLARLLQAEPDGERVAGLVAAALGEPMVSEGGEETLWAVRRLVEALA
                     AAGPLVLVFEDIHWAEPTLLTVIEHLAERIRERAVLLLCLARPELLDDRPSWGRGKVD
                     GASLLLKSLSDSAAQQMLQERLEGRPIAPGVRTRIMQSAQGVPLFLEQMLALLEEHDS
                     IGVEDVPPAISGLLSARLESLEQVDRALLEEASVEGERFHAGALAALAADAPASVSAR
                     LDGLEAREMIRPDDADIAGERGFRFAHVLIRDAAYERVPKVERAVLHERLAAWLEDHG
                     TGDELVGYHLERAFRLLAELAPPDERAEALARRASRLLAAAGRRGQGRVDYPAAVNLL
                     SRAVNLLPVDDRERLELLPDLAFLLGEQDVSSSLPLLTEAIQRARATGNRRVEWRSAM
                     IRSRVLLYNDPSSRPAAEVLAEAERATEQLEALGDKRGVASALVVRADVHEMAGELSR
                     CYELYRRTAALSAGGVRARALGYAGWGLCLGTTPANEVEVGCLELLEAAGESHVDRAL
                     VLTSVGWAQAMQGRLGEGTQASTAGRDAVVEHGTADWLGVATLACGYVALLCDDLVAA
                     EREFARAAETFAAQGDRWYLAVAAVDRALVLCALGRHTEALAVAASSRAPTDAEWATK
                     WSRVQGLVDACEGWLDTALAHADASVSAAQATEYTNYHAAALADRAGIRDRLGHVREA
                     VADLKDARVLYERKGNLVDADRIRRRLEASARRVSSRSL"
     gene            15079..15432
                     /locus_tag="VF752_08205"
     CDS             15079..15432
                     /locus_tag="VF752_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8855571.1"
                     /translation="MEPQPIVVPPGQGHRIGNVEFLARTVDTPRFTFGIIEIAPGREL
                     EAHVHNEEDDAFYIVEGEMTFFFGDEEVTATPGTFVLVPPGVEHGFRNDNAEPVRFLN
                     IHAPAGFDRRIGLPD"
     gene            complement(15436..15600)
                     /locus_tag="VF752_08210"
     CDS             complement(15436..15600)
                     /locus_tag="VF752_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855572.1"
                     /translation="MVFLDASHRGRGKASGIEVGGQTGYLYTVRDGKIVRVELYPTPA
                     DALEAAGVRE"
     gene            complement(15624..15839)
                     /locus_tag="VF752_08215"
     CDS             complement(15624..15839)
                     /locus_tag="VF752_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855573.1"
                     /translation="MSQDDVRVVLDQYAATNERDFARAMGYYADDVVLVVHPEAFVEG
                     GTFEGRDAVGQWFANWFTTFEPATTSK"
     gene            complement(15856..16239)
                     /locus_tag="VF752_08220"
     CDS             complement(15856..16239)
                     /locus_tag="VF752_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855574.1"
                     /translation="MPETSLRSRILDPVIMKLRLVPGQPVDDDREAVASSLVGVTPLE
                     YEGAAYVVANRAETRWARTLRAVGRGEFIGWLGNKAFRAVEVDGRERERVVTTYRNAL
                     GRLARDYFEQFPNPADHPTFRLEPI"
     gene            complement(16242..18446)
                     /locus_tag="VF752_08225"
     CDS             complement(16242..18446)
                     /locus_tag="VF752_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8855575.1"
                     /translation="MDSPHAAPQARGLVARIAGWTMTHRRATVVGWLVILIVTLAVSG
                     AVGSHYSNNNSLPGTQSQAATDLLQRDFPSQAGDSDRIVLHVREGRITDPAVRAHIAP
                     MLAKVAKLPHVTGVVSPFTRAGAEAISRDGRTAFATVTFDERANTLPKAATKRVISTA
                     EGARSPQLQVELGGQAIEQAQGVSFGTASAVGLGAAIIVLLITFGSFLAAGLPIVTAL
                     LGLGSAFGLIALGSRVLSMPDFSTELAAMIGLGVGIDYALFIVTRFRESYRSGGDVQA
                     AISQAMDTAGRAVLFAGATVILALLGMLALGVTFLTGLAVSSAIAVLLTMLAALTLLP
                     AFLARAGRRIAERGRAPSTVVSDAAAAGFWHRWAELIRRRPWPAAIAGLLIMLVIAAP
                     ALGMRMGLSDQGNAPKSETTRQAYDLLAAGFGKGFNGPLLVVASLPSKNDQAALQRIS
                     ATVRSNPDVASVAPAQISPNGRTAVIEAFPRSSPQSTATSDLVKSLRDDVLPPVAHST
                     GTTLMVGGVTATGIDFTNVLSSKLPLFVAIVVGLAALLLLVVFRSLVIPVQAALMNLL
                     SIGAALGVTVAIFQHGWLGGLFGVQPGPIEPWLPVILFAIVFGLSMDYEVFLVSRIHE
                     EWTKRRDASRAVVNGMASTGRVITAAATIMVCVFLSFVFGPDRDIKLFGLSLASAVFL
                     DAFVIRSLLLPSVLELLGRNTWKFPDWLHRRLPRLAIEEPSARPPKPVLEEG"
     gene            18537..19088
                     /locus_tag="VF752_08230"
     CDS             18537..19088
                     /locus_tag="VF752_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8855576.1"
                     /translation="MAVKTEAKGSRVGLVDAIAGQEPISDDLRELLGMVRPVFMGMKR
                     GGPVPAPLENAFEEAGLGPRHVAPLMAVTLEGSLSVSELAAQLGLSLSTTSLMVGELS
                     RAGLLERKECEDDRRRTLVHVNEKYEESLRNWLAERMEPFRRTLERLSPRARAGFLEG
                     WRVLAEETARIGGESEAGADAKA"
     gene            complement(19108..19359)
                     /locus_tag="VF752_08235"
     CDS             complement(19108..19359)
                     /locus_tag="VF752_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855577.1"
                     /translation="MGTELGPTRPNRLTIWPVEDDSFGIDVRWGGAAGNRRATVVARL
                     LSDAGIHGKLRQTIDGAWEIRVGPVPGDEVAKVIDSFVW"
     gene            complement(19410..20606)
                     /locus_tag="VF752_08240"
     CDS             complement(19410..20606)
                     /locus_tag="VF752_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8855578.1"
                     /translation="MSATGTRPAMTAGDFLDLDASLSDEERLFRDTVRAFVQAELLPH
                     VPDWFEEGTLPREIVAELGKLGVLGMHLEGYGTAGASATLYGLACQELEAGDSGLRSL
                     VSVQGSLAMYAIWRYGSEEQKQHWLPRMAAGEAVGCFGLTEADAGSDPASMRTRARRD
                     GSNWVLNGAKMWITNGSMSDVAVVWARTDDGIRGFIVEQGTPGFTTQDIHRKLSLRAS
                     VTSELIFDDCRVPADAQLPEATGLGAPLSCLNEARYGIVWGSAGAARACLEAAVEYSV
                     TREQFGAPIAALQLTQRRLAEMLRKVTTAQLLALQLGRMKDEGRLRPEQVSLGKLNNV
                     GAALEVAREARQLLGANGVTLEYPVMRHANNLESVVTYEGTAEIHTLVLGAALTGRPA
                     YRPPTP"
     gene            complement(20603..21832)
                     /locus_tag="VF752_08245"
     CDS             complement(20603..21832)
                     /locus_tag="VF752_08245"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8855579.1"
                     /translation="MSRQPTRYDMTQSGIPSSALPKALGHIRVLDLSRVLAGPYCTML
                     LADLGADVIKVEHPVGGDETRSWGPPFAGGEATYYLSVNRNKRSAALDLKDEDDLAAV
                     RRRAEGSHVVIENFRQGTAERLGLGYEQLRELNPALVYCSITGFGSARAPEGRPGYDF
                     VAQAETGLMSVTGPLDGEPSKVGVALVDVLCGLHAAVGILAALRRAEQTGVGEHVEVS
                     LIDAGLASLVNVAQGVLATGEEARRYGNAHPSIVPYEPFETREGWLAVAAPNDGLWRR
                     LCEAIERPELADDARFATNELRVRAREELVPLLTEAFRARTADEWLELLEAAGVSAGK
                     VRGVGEAFDAAEAAGDPATVEVEHPTAGTLRLARSPIRLHETPDSRDSAPPLLGEHTA
                     EVLRELGIPHDENGEKI"
BASE COUNT         3491 a         7762 c         7444 g         3225 t
ORIGIN      
        1 cgatcgcgcg cgtggtgctc gagggtcgcg cgcgaacgct gccgcgtccg gtcactgctc
       61 ccaagcggcg gctcggccac tcgctcgcgc tcggcacggc gtgcgtcgcg ctactcgcgt
      121 tctcgctcag cagcgacgac actttccccg tgttcgcgcg ggctttcgac ctgcatcccg
      181 agaccgcggt ggccaccacg aggcccgagg tggggctgct cgtgcttgtc gatccgagcc
      241 aggtgccggc agccgcccga gcgctcgagc ggcgcggcat gcgcgcatcc ttcgtggttg
      301 cgacgatgcc ccgacctgga ctgatgagcg cgctgcagcc ttacggcgac gatgcgattc
      361 cccagctcga cagccgcgcg ttcgtgcact gggtcggcgc gaagcgcgag cttcgccagg
      421 tggcgacggc ggtcggctac ggcaagcact accgctacgt gtcgggcgac ggattcagcc
      481 tcggcgagta cgtgctcgcg cactcggccg gtggcttgcc ggtcgggccg gccgtcacga
      541 tcaccggccc ggcgacccag aagctggccg ccgggcagct tgtgcaggtt cgcctcgcgg
      601 gtccgcagag cacctgggcg cccacgctcg acgcgctcgc gtccgactgc caggcggccc
      661 ggctccatac gctctcggcg cggcagctgt tggtgtcgag cggcgatcgg tccagcgacc
      721 agctggcggc gagcgggtag cgcgatgcgc gtcgcagcgc tggcagtcgg ggcgttcgcc
      781 gtgaactcgc tgccggggct cgcgccgctc gttgcgccgg tcgcggacgt gctcggcatc
      841 gcgcgccgca tcgaccgctc ccccggcgtg gcgctcacgt tcgacgacgg gccccatccc
      901 gacggcacgc cgggcgtgct cgagctgctc cgacgagcgg gcgcgacggc gacgttcttc
      961 ctcgccggag agcaggtgga gctgcggccg gcgctggccg ccgagatcgt cgcggcgggc
     1021 cacggcgtcg ggctgcattg ccaccgccac cgcaacatgc tgcggctcac gcccgcgcag
     1081 atcgccgatg acctgcgccg cggagcggcg accatcgagg acgcgaccgg ccactccccc
     1141 aggctgcatc ggccgcccta cgggatctac accgcactcg ggctcgccgc cgttcgcacg
     1201 cgctggcggc cgctgctgtg gtcacgctgg ggccgcgact ggcgcgcgcg gatcagcccg
     1261 cacgcgatcg cgcgcatggt cacgcgcgac atcggaccgg gcgacgtatt gctcctgcac
     1321 gacgccgact ggtacagcgc gccggggtcg tggacccgca cgctggcggc gctgcccgag
     1381 gtgctcggcg caatcgaggc gcgtggcttg aagacggtgc cggcggcgta gggggagaga
     1441 acctccgccc gcggccttct agtgacatgg gccgcctgga ccgcatcccc ttcgtcgagc
     1501 tccccggcgg actgcgcatg cacgtcgccc gggacccgat ctcccgcctg ttcgggctcg
     1561 cccacctcga cgagatcccg ccgcgcagcg cgctcacgct cccgcattgc cgctcggtgc
     1621 acacctacgg catgcgcttc ccgctcgacc tgatctggat cggcgacgag ctgcggctcg
     1681 accgcgaggt gccgccgaac cgggtcgcac ccggtcgcgg cgggcgccac gtggttgaga
     1741 cgcgcgccgg cgagggagag ctgttcctag aaggcctgcg gctcgcgctc gagcactgag
     1801 cgcagccgga agctgagcgc cgccagggcg aggtcctcgc tcacgttcaa ctcgagcgag
     1861 ctgcgcgcct cctcgcatat ctcggccgcc tcgcgcagcg cgaaggggtc gagcacgcgg
     1921 ccgctggccg cggcctggag cgcctcgatg cggtcgcggc cgagcacggc ctctcccgcg
     1981 ccagtctgca ccgcgagcag gtcgcggaag ccgatcgcgg cgagttccaa gcccgtgtcg
     2041 agcacctcgg tgcgggcgcg gcggccgtcg cgcttcgcgg cctcctcgaa ctcgcgctcg
     2101 atcgcgcggc gctcgcggcc cttcggctcg agttccagtc gctccttctt cgcgcgctcg
     2161 accgcctcct ccgccgcgac gcgacgctcc tcagcgcgct cgagcagtgc tcgccagggc
     2221 tccttcgcgg cacgcgccga gtcgtcgctc gcgagcgcgt ctgccacgag cgcgtcggcg
     2281 tcgcggcgaa gcgcggcgcc ctcctcggtc gcaaacacgc gcgcccgcgc gccgttgccg
     2341 agcgacaccc gcgcgcagga gtcggcgacg tccgccggca cgccgtcggc gaccagcccc
     2401 tcggcgatcc gttccggcgg cagcgggtcg aagcgcacca cctggcagcg cgacaccacg
     2461 gtctcgagca cgccgccgag ctgctcggtc agcaggatta ggtgcacgaa cggcggcggc
     2521 tcctcgagcg tcttcagcat ccggttcgcc acctcgtcgt tcatcgtctc cacgcgctcg
     2581 agcacgaaca cacgccggct cgactcaaac ggtgtgcgcg tcgcggcggc cacgaccggc
     2641 tcgtcgatgt cgtcgacgcg catcacgtgc gcgccggtgg gcgcgaccca ggtgagatcc
     2701 gggtgcacgc cgtgcatcac gcgcgtcctg acgctctcgg gatcgggcgc gccctcggca
     2761 agcagctcgg ccgcgagcgc acgggcggcg gtgcgcttgc ccgtcccggc cggtccgtgg
     2821 aagaggtagg cgtgcgaggg cgagcccgag gcgagccccg cgccgagcac gagccgggcg
     2881 tgcgggtggt cctcggtgcc cgcgatcagc ggcgcgctca tacggccacc cgcgagcgcg
     2941 cgcgcaccac cgcgtcgagc acgtcgccgt gcacgtcgtc gggcgaacgc gaggcgtcca
     3001 ccaccaccca gcgcgatggg tcggccgccg cgagctgcgc gtaggtgtcg gccactgctc
     3061 gctgcaggtc ggtgccttcg tcctcgaagc ggtcggcctc gccggcgcgc gacgcggcgg
     3121 tctccgggtc gatgcgcagg aggatcgtga ggtcgggcac gacaccgccg gtcgcccact
     3181 cgttgatgcg cgccacgccc tgcaggccga gtccccgcgc cccgccctgg tacgcgagcg
     3241 acgagtcgag gaagcggtcg gacaccacga cgaggccctg ctcgagcgcg ggcgcaatca
     3301 cctgcgccac gagctccgcg cgcgcggcgg cgaacagcag cgcctcggcc tcggcgccga
     3361 tctcgacgcc cgggtccttc agcagctcgc gcactcgctc gccgacggcg gtgcccccgg
     3421 gctcccggac tgaaatcgcc tggtcattgc cgagcgcgtc tacgagcagc tgcgtctgtg
     3481 tggtcttgcc cgagcggtcg atgccctcga gcgtcacgaa cagtcctcgc atgcggcgat
     3541 cgacggtacc agccgctggg gcgcgcacta ggatggcgcg ctcatgcgag ccgtggtcac
     3601 aggcggagcg ggcttcatcg gctcgaatct cgtcgacgcg ctgctggcgc gcggcgacga
     3661 ggtcacggtc gtcgacaacc tctcgaccgg ccgccgctcg aacctcgacc aggcgatcgc
     3721 gggcggcgcc gtgctggccg agatcgacat ccgcgaggcc gacgccgtgc gcgacctcgt
     3781 cacgcgcgtg cagcccgagg cggtcttcca cctggcggcg caaatcgacg tgcgcaagtc
     3841 ggtcgccgac ccagccaacg acgcgcgcat caacgtcgag ggcacgatca acgtgctgaa
     3901 cgccgcgctc gccgccggcg tgcggcgttt cgtgaacacc tcgacaggag gcgcaatcta
     3961 cggcgagggc cgcgtgctgc cggcgcccga ggaccacccc gtcgcaccgg aggcgccgta
     4021 cggccagtcg aagttcgcgg ccgagggcta ctgctcgctg ttcaaccgcc tgcacggcct
     4081 ctcgaccgtc tcactccgct acggcaacgt ctatgggccg cgccaggacc cgcttggtga
     4141 ggcgggcgtg attgcgatct tctccggcaa gttgatcgag ggcggccgcc ccaccgtgtt
     4201 cggcagtggc gagcagacgc gcgactacat ccacgtggac gacgtcgtgc gcgcgaacct
     4261 cctggccgcg gactcggacg aaaccggcgc tttcaacatc gggagctgcg aagaggtgag
     4321 cgtgctcgaa ctcgtggacg cgttcagaga gatttcgccc gatagcttcg atcccgagtt
     4381 cgctcccgag cggcccggcg aggtgcagca catctacctc gactattcgc gcgcgcgcga
     4441 ggggctcggc tgggagccgc aggtgacgct cgacgagggc ctgcgctcga cggtcgagtc
     4501 ggtacgcgag tcgcgctagc ttgcgggcat gaccgtcgtg tgggtcgcga tcgcggtggt
     4561 ggtgctcgtc gtgctgggcg cgctcgtcgt ggcgcgcacc cggggcatca ctcgcaccga
     4621 cgcgcgcgag cgcatggcag acaccgcggc cgatttccgc gactggatca ggctagggcg
     4681 ctagctcacc cgcggcgatc gagcagcgga tgcaccccgc gggtccgcgg gatcttgacc
     4741 ttcaccaccg accagctgtc ggtggccaaa cgtcttggca gccaggcatg agtcgcacgg
     4801 tccgggtgct catggcagag ccgaacgcag agcgcctcgg cttcctccct tctcatggcc
     4861 gtgaaggcta cgcccttccc gcaaacacgg agccaattct ggcgcgggaa cccgcaaact
     4921 gggcaaaaaa ccgtattcag gcacaagatg ttgtgtttcg gagaggaatc gagcacaaca
     4981 cctagtagaa ttaggggctg tgcgctgccc ctattgctcg tttgaatcga cccgcgtagt
     5041 cgactcgagg ctcacggagc cgggcgacgc cattcgccgg cgccgcgagt gcgcgagctg
     5101 cggccagcgc ttcaccacct tcgaacgggc cgaagggccc acgctcgtcg tgcagaagcg
     5161 cgacggcagc caggagaagt tcgaccggca gaagctcgtc gacggcatgc tgcgcgcggc
     5221 ctcgaagcgg ggcgtggatg tggccgatct cgaggcgata gccgactcga tcgcaggcga
     5281 catccggcgg gcgggcggct cgatgagtgc gcaggagatc ggcgagcgcg tgatgcgcgg
     5341 gctggtcgcg ctcgaccgcg tcagcgcgct gctgttcgcg gcggtttacc ggtcgttcga
     5401 cgacctcgac gacgtcgagg ccgaggtgcg ccgcatcgag tcgcagagcg tcgcgccggc
     5461 gaatcagctg gacctggccg agcccggatc ggttctgtcc gacccgcgag caagcatcgg
     5521 tccttccccc tcagacccgt ctggggagac gcgagaagca tccgagatcg ataggagagg
     5581 ccatgcagtc cagccgtagc gccgccgcac cacccaccca ggtgagcacc gatggagtcc
     5641 agatcgggcg gcgcttcact caggctggag agcatccgtt cgacatggtc gagtgggaga
     5701 tccgcgatgc cgtgatcggc aacccggaga gccccgcctt cgagcagcac ggcgtcgagt
     5761 ttccggcgag ctggtcgcag aacgcgacca acatcgtcag ccagaagtac ttccgcggga
     5821 agctcggctc cgagcaacgc gagtcgtccg taaagcagat ggtcggccgc gtcgccggca
     5881 cgatcgccga gtggggccgc gagggcggct acttcgcgag cggggacgac ggcgacgcct
     5941 tcgaggccga gctcacacac atccttctca accagtacgc ggcgttcaac tcgccggtct
     6001 ggttcaacgt cggtttcgag gagcggccgc agtgctcggc ctgccagccc taccacgcac
     6061 tcatctccac gccggacggc ctcgttccaa tcggcgaact cgtgaccgaa gatcaagtcg
     6121 gtcgcgaggt ttacgacgcg gagggaacta cgcgtatcgt ggcgacaaag gccaacggcc
     6181 gcaaggccgt ttggcgcgtc cagcttcgca acggtagcta tgtcgaggca accgcggacc
     6241 atgtcgtcag ggcggtcggc gagcggcgca gcgcccttga atggctacgg gtggatcagc
     6301 tggagcccgg gatgaggatg catctccatc cgcaccgagc caaggtcgca gcgccagcgc
     6361 ttgtgacagc tggcggtgca agcggggtcg atgagccagc gtccgccgat gcggtggctg
     6421 tcgcggaagc agcgcttgcc ggctggcttc aggcagacgg cttcgtcggc cagtacgacg
     6481 gaaccaacag gtcgctgacg attgaattcc aagtcgcgga cgacgcggag tacgagtggg
     6541 tcatggacaa cctggatgtc gtcttcccgg acgtccaccg aaaggttcgc gaggccgaca
     6601 ccaaggacac ccgtgttcag cgcatccggc tctatggaga ggtgctgcgt gcgttcgtcg
     6661 agcgctggga ccttttggcc cgcggtaccg agatccgcgt cccgtctcgc ctgtggacgg
     6721 cttcccacga cgagatagcg gcctatctga ggagcatttt ccaggccgac ggctacatca
     6781 gccttcgcca cgacaacggt tgcgagaacg cgcgaatcgg gttcgccgtc attggcgagc
     6841 gctggactga ggacgtgcag ctcctgctcg gcgtgttggg catctactca aggcgcacac
     6901 gcaagttgga gcgtcgtccc gacaggcacg acctacacga ggtctcggtt tcgatcggtt
     6961 cggagcgagc ccgcttcgcg gagctggtgg gctttgttag tagggcgaag caggagcggc
     7021 tgctctcttc gctggagctt cgcaatccca agcgttgccc ggatctgcgc gaggaggaaa
     7081 tcgtctccat cgaaccgctc ggcgatatgg aggttttcga catccagact gagtcgggcg
     7141 agtacctcac caattacatc gcggtccaca attgcttcat cctcagcgtc caggacacga
     7201 tcgagtcgat cctcgactgg aacaccaagg agggcatgat cttccgcggc ggctccggct
     7261 cggggatcaa cctctccaac atccgcggct cgcaggagca tctctcgaag ggcgggcttg
     7321 cgagcggccc cgtctcgttc atgcgcgggg cggacgcgtg ggcgggcacg atcaagtccg
     7381 gcggcaagac gcgccgcgcg gccaagatgg tcgtgctcga cgtcgaccac cccgacatcg
     7441 aggccttcat ctggtgcaag gcgcacgagg aggagaaggc ggccgcccta cgcgacgccg
     7501 gcttcgacat gcgcctcgac agcgaggcgt tcgcttcgat ccagtaccag aacgcgaaca
     7561 actccgtgcg cgtcaccgac gagttcatgg agcgcgccga gcgcggcgag gactgggagc
     7621 tgaaggcgcg cgtgggcggc gagaccctga agacggtcga cgcgcgtgac ctgctgcggc
     7681 agatctccga cgccgcttgg cgctgcgccg atccgggcgt ccagtacgac acgacgatca
     7741 acgaatggca cacctccccg aactcgggcc gcatcaacgc gtcgaacccg tgctcggagt
     7801 acatgcacgt cgacgactcg gcctgcaacc tcgcgtcgct caacctcatg aagttccgcc
     7861 gcgaggacgg caccttcgac gtcgagacgt tcgagcactg cgtcgacgtc gtgatcctcg
     7921 cgcaggagat catcgtcggc ccgtcaagct acccgaccga ggagatcggc aagaacgctc
     7981 gcgcgttccg ccagctcggc ctgggctacg cgaacctcgg cgcgctgctg atgtcgaacg
     8041 gcctgccgta cgactcggac gagggtcgcg cgcaggcagc cgcgatcacc gcgctgatga
     8101 ccggccgcgc ctaccgcaag tcggccgagg tggccgcggt gatgggcccg tacgaccgct
     8161 acgaggagaa ccgcgagcca cataaccacg tgatgcgcaa gcatcgcgat gcggcctacg
     8221 agatcgacgc gctcgtcgcc gagcgcgaac tcgtcaaggc ggcgcgccgg gcctgggacg
     8281 aggcggtcga actcggcgag gcgcacggct accgcaacgc acaggccacg gtgctcgcgc
     8341 ccacgggcac gatctcgttc ctcatggact gcgacacgac cggcatcgag cccgacttct
     8401 cgctcgtcaa gttcaaggag ctcgtcggcg gcggccagat gaagatcgtg aacaacatgg
     8461 tcccgctggc gctgcgcaca ctcggctaca gcgaggagca ggtcgagcgg atcatcgcgt
     8521 acgtgaacga gcgcgccacg atcgtgggcg cgccggagct ggcgcccgag cacttgccgg
     8581 tgttcgacgt ggcggtcggc gagcgggcga tctcgcacat gggccacatc aagatgatgg
     8641 gcgcggtgca gccgttcatc tcgggcgcga tctcgaagac ggtgaacatg cccgagacca
     8701 ccacggtcga ggacatcgcc gacgcatacc tgcaggcttg gaagctcggc gtcaaggcac
     8761 tcgctatcta ccgcgacggc tcgaagacgg cgcaggcgct gtccacgagc ggctcgaaga
     8821 agaaggacaa gaaggcggag gccgaggagc ctgttgcgga tccgcgcccg caccgccgcc
     8881 ggatgccgca ggagcgccag tcgatcacgc acaagttccg gatcggccag ggtcacgagg
     8941 gctacatcac ggccggcatg tacgaggacg gctcgctcgg cgagatcttc ctgacggaca
     9001 tcggcaagga aggctccacg ctgcgcggca tgatgaattc gttcgcgacc gcgatctcga
     9061 tcgggctgca gtacggagtg ccgctcgaca cctacgtccg caagttctcc tacatgcgct
     9121 tcgacccgga gggcatgacc acgaacccgg agatcccgtt cgcgaagtcg atgcccgact
     9181 acatcatgcg ctggctggcg tcgcgcttcc tggacgtgga cgtccaggag gagctcggca
     9241 tcctcacgcc cgaggtgcgg gcccggaagg ccgcgcacga cgcgctcatg cgcggcgaca
     9301 ccgcggcgcc cgcagcggag ccggagacga ccgaggcgaa gcccgccaac ggcaatggca
     9361 cgaatggcgg caacaccgcc gcgaagcccg cggcgtcagc gctgaccgac acgcctccgg
     9421 tgctgccggc gaagctggcc ggcctcgacc tgggcccggc ctgcgagcag tgcggcggga
     9481 tgatgcagcg gactggcagc tgctacacgt gctccagctg cggcaacaac acgggctgcg
     9541 gttgatcgac agcgcctgac acataagtgg ccgaggggga gccaacccgg ctccccctct
     9601 catttacgcg tgctccagag gcggtcgcgc tagtgcgcac actcgagaag ctccgccgtc
     9661 gaacgctcga gaccgtccgc gagcacggcg acgtccggaa cggcatcgcg gtcggcgcag
     9721 agcccgaagt ggatgctgcc gcaggcggac accactgcca cccgcagcgc gtgtcgctgg
     9781 gccacttcca caagcgagta catctcgcgc accctggctc cgagcacgta gaccggttgg
     9841 gcgggcccgc gcacgtttga cacgttgaag gtgaacactc gcgggctcat cgcccagtgg
     9901 gcgacgggcc ggtgcagacc gaggcggtag agcgcctccg cgtcgcgatc gaccttgcgc
     9961 tccgtcgtct cccggttgat cgcgagtaca cgtttcaccg ggtccggctc cccgaccggc
    10021 agatcgacga agaagtacga atcgcggttc gcctcgcggt cgccaccggc cttgtcgtgg
    10081 aggctcacgg ggaccttgac gcgcaccact tcgtcgtgcc cgtgccgatg cgcaatccat
    10141 tcgcgcaccg ccccggccac gatggcgagc accacgtcgt tcacggtcac ggcgtcgtga
    10201 atcgctttgc cggcccggcg gcagtcgtcc agcggagcgc gttggaacgc gatgctgcgc
    10261 gtggaaccga ttcgtctgtc gagtggagag cgcgacgctg ttcgcgcgag ctcgcgcctg
    10321 atcgcggccc gcgatcgcaa tacagaagtg gcttggtcct gcggccgccg tcccctgcgt
    10381 ggaacccgct gggccgcctg gcgtgcgcgg tcccgtagcc cgcgagcgag caggccggcg
    10441 gaaccggggc aggcgtcagg gctccagttc ggtgacgcgg gcacgacgtc atcggggtgc
    10501 tcgctccaga ggacatcgga accgagccgc accgccgtcg tgccatccgc gaggcagtga
    10561 tggagccgcc atacaagcgc catcgagccg tcggtgagtt cgtcgatcac gtcgagttgc
    10621 caaagcggat gcgagcggtc gaggcgctct tccatcaggc ggccaacgat ctcgtcgagc
    10681 tgcggcgggc tcacagcgcc gcgggtctcg acgggcctca cgtggcgcgc aatgtcgaac
    10741 tcctggtcat cgacccaagc aggcggcgcg aggtgcagcg gcgtggaggc gaggcacctt
    10801 cgcagtcgcg gcgccgcgtc aagccgagtc gacacgtgct cccggagcgc atccgtggaa
    10861 gggagcgagc catgctcacc aggctcgagc agaatcacct tgcaggtgtg tccgcgcacc
    10921 gtcccgcgct cgagtctcag gattcgagcg tcgtcggcgc tgagtctgtc gagacgttcg
    10981 gcgcgcacct tcgtgcgaca ggagcctgcg gcgagtcggg gtttgatgcg cagcttgatc
    11041 gccggctctc cggcagtcaa cggcaaggcc gcagttcttc aagccgacct aagtctcgtc
    11101 ggcgagtggg atcaatggtc gggcgctcgt cgtcttcagg agaaacggcc ctccctcccc
    11161 caaccaaagg accgaccaaa tgtcactcca catccgcagg atcatcgcgg gggcaatcgc
    11221 caccgcatcg ctgcttgcgt tctcggttcc cgcgctcgtc catgcgagta gcgctacgaa
    11281 ggcgcactcc gtcagcgcgc acgccgccaa gcggcatcac cgccaccaca gccggatccc
    11341 gcagcacaac ggcggagatc acgacgccga caacaacggc ggaccgagcg atggcgacgg
    11401 caacatttag ccgcctcgtc cccctcaccg cggtcgccgc agccctcgtg atagcgggct
    11461 gcggcggcgc ggccgctccc aaaccggccc acccggccgc cttgacgccg ggaccatttg
    11521 cctgggtgcg tcccgccgag gtgccggcaa attggaaggt cggacagctg ccgtcgcgca
    11581 aggccgagct cgcatacccg ccgcagtggc gggccataac cacggatccg ggcacgttct
    11641 ccgccgcgct gctaggagcg aacggcgcga ttcgcgggta cctcaacgcc acgccccagt
    11701 cgggcgcgga gacactcgcg aactgggcaa agtttcgacc ggctcacaac ggtgaagagg
    11761 gtgaccgcag cgtcgtacgc gaagcgtcgg caacaggatt gcccttccgc tcggccaccg
    11821 gctcgtgcgt gatggatcac tacacgacca cgtccgcgcg ctatcgggag atcgcctgca
    11881 tcgtgcgcgg cgcgcgtgcc accacggtcg ttgtcgccgc cgctcagccc ggcgactggg
    11941 cacgccttcg gccgcagctc gagcgctcga tcacaagctt cgcgagctga cccgacgcgc
    12001 ggaggcctcg agtcgcctgc ggatccggtc agcgtcgacg aggttgccct tcctctcgta
    12061 gaggacgcgc gcgtccttca ggtcagcgac ggcctctcgc acgtgaccga gccgatccct
    12121 gattccggcg cgatccgcca gcgccgcggc gtggtagttg gtgtattcgg tggcctgcgc
    12181 cgcgctcacc gaggcgtctg cgtgggcgag tgcggtatcc agccagcctt cgcaggcgtc
    12241 gacgagtccc tgaacgcggc tccacttggt tgcccattcc gcgtcggtgg gcgccctgga
    12301 gctcgcggca accgcgagcg cctcggtatg gcggccgagc gcgcaaagca ccagagcgcg
    12361 gtcgactgct gccaccgcga ggtaccacct gtcgccctgc gcggcgaatg tctctgcggc
    12421 gcgggcaaac tcgcgttcag cggcgacgag gtcgtcgcag agcagcgcaa cgtaaccgca
    12481 cgccagtgtc gccaccccga gccagtcggc ggttccgtgc tcgaccacgg catcgcgacc
    12541 ggcggtggag gcctgggtgc cttcgccgag tcggccctgc attgcttgtg cccagcccac
    12601 tgaggtgagt acgagcgcac gatcgacatg actctcgccc gccgcctcga gcaactcgag
    12661 gcatccgact tccacctcgt tcgcaggggt cgtaccgagg cacagccccc atcccgcgta
    12721 gccaagcgcc ctcgcgcgaa cgccgcccgc gctcaaggcc gccgtgcgcc gatacagctc
    12781 gtaacagcgc gagagctctc cagccatctc gtgaacgtcg gcgcgtacga caagagcgct
    12841 cgcaaccccg cgcttgtctc cgagcgcctc gagctgttcg gtcgcgcgtt ccgcttccgc
    12901 caacacctcg gccgccggtc gcgaagatgg atcgttgtac agcagaaccc gcgagcggat
    12961 catggcgctc ctccactcca ctcgtcgatt cccggtcgcg cgcgctcgct ggatagcctc
    13021 ggtgagcaac ggcagcgagc tcgagacgtc ctgctcgccg agcaggaagg ccaggtccgg
    13081 cagcaactca agtcgctccc ggtcatcgac cggcaggagg ttgactgccc gcgagaggag
    13141 gttcaccgcc gccgggtagt cgacgcggcc ttggccgcgc cgaccagcgg ccgccagcag
    13201 tcgcgaggcg cgccgcgcga gagcctccgc ccgctcatcc ggcggcgcga gctcggccag
    13261 aagccgaaac gctcgttcca ggtgatagcc gaccagctcg tcgcccgtgc cgtgatcttc
    13321 gagccatgcg gcaagccgct cgtgcagcac agcgcgctcc accttgggca cccgctcgta
    13381 cgccgcgtcg cgaatcagga cgtgggcgaa gcgaaagccc cgctcgccgg cgatgtccgc
    13441 gtcgtcaggc cggatcatct cgcgcgcctc cagaccatcg agccgggcgg acacgctcgc
    13501 aggtgcatca gcagccagcg cggcaagcgc gcctgcgtgg aaccgctcgc cttcgaccga
    13561 agcctcctcg agcagcgccc ggtccacctg ctcgagcgac tcgagccggg cggacagcag
    13621 cccgctgatc gccggtggca cgtcctccac gccgatcgag tcgtgctcct ccaagagcgc
    13681 cagcatctgc tccaggaaca gcggcacccc ctgagccgac tgcatgattc gagtacgcac
    13741 cccaggtgcg atcggccggc cttcgagccg ctcctgcagc atctgctgcg cagcgctgtc
    13801 tgacagggat ttcaggagca gcgaggcccc gtcgaccttg ccgcggcccc aactcgggcg
    13861 atcatcgagt agctcgggcc tcgcaaggca cagcaggaga accgcccgct cgcgaatgcg
    13921 ctcagcgagg tgctcgatca cagtgagcag cgtcggctcc gcccagtgga tgtcctcgaa
    13981 caccaaaacg aggggccccg cagcagccag cgcctcgacg aggcgcctga ccgcccagag
    14041 cgtctcctcg cctccctcgc tcaccatggg ttccccgagc gccgcggcca ccagccccgc
    14101 gactcgctcg ccgtcgggct cagcctggag caggcgggcg agtccggagt atgtgttgac
    14161 gccagcggcc tcgcgcacca tctccgcgac cggccagtag gtgatgccct cgccgtacga
    14221 gcgacagtgg ccaaccagca cctgggcttc ccccgccact gacgcagcaa gctcgagcgc
    14281 gaggcgggac ttgccgatgc ccgggaagcc gagcacggtt gcaagtacgc agcgccgctc
    14341 cgcaaccgcg cgctcgaacg ctcggcgcaa gagccgaagc tcgcgctcgc ggcccacgag
    14401 cgggcgatcg aactcccgag tcagcggttc ggcagtgggg tcgatcgcca gcagcctcca
    14461 cgcggtcact cgcgaacgca tgcctttgag ttccatgccg gcgaccggct cggtggtcac
    14521 tgcatggcgc accagccggt gcgtgtcgtc cgcgatcaag atttcatcgg gcgctgcgat
    14581 ctgttcgagc cgagccgcgg tgttcatcac atcgcccagc acgacctcgt catctgcgcc
    14641 cagcacgacc tcgccggtgc agacgccgag atgcgcctcg agctcgacgt cgaactgggc
    14701 tcgcagggag cggttcagct cggccagccg attgcgcatc tcatccgcag ctcgcacggc
    14761 gcgcagcgca tcgtcttcgt gcagtcgtgg gaccccgaac acgcccacca cggcatcgcc
    14821 gatgaacttc gccaccaatc caccgtgcgc ctcgagtacg ccgcgcatct cctcgaagta
    14881 acgccgcatc accaggcgca gtgcctcggg atcaagacgc tgtcccaacg acgtcgagcc
    14941 gaccacgtcg cagaacacgg cggtcaccgt ctttcgcacc tcgctacgag gtcccgaggt
    15001 cgtcaccgag tcagtctcgc gggcaccaac gagcgcgtaa aggtcctgat gtctcaagcg
    15061 tttggaagga tccgcgcgat ggagcctcaa ccgatcgtcg ttccgcccgg ccagggtcac
    15121 cgcatcggca acgtcgagtt cctcgcgcgc acggtcgaca cgccgcgctt cacgttcggg
    15181 atcatcgaga tcgcgccggg ccgcgagctc gaggcccacg tgcacaacga ggaggacgac
    15241 gccttctaca tcgtcgaggg tgagatgacg ttcttcttcg gcgacgaaga ggtgacagcg
    15301 acgcccggca cgttcgtgct cgtgccgccc ggcgtcgagc acggcttccg caacgacaac
    15361 gccgagccgg tgcgcttcct caacatccac gccccggcgg gattcgaccg ccgcatcggc
    15421 ctccccgact gaggcctatt cccgaacccc ggcggcctcc agcgcgtcgg ccggcgtggg
    15481 atacagctcg acgcgaacga tcttgccgtc ccgcacggtg tagaggtagc cggtctgacc
    15541 accgacctcg atcccgctcg ccttgccacg tccacggtgc gacgcatcga gaaataccac
    15601 ggcgcccagg tcgcgcgctt cctctatttc gaagtggtag ccggctcgaa cgtcgtgaac
    15661 cagttcgcga accactgccc cacagcgtcg cgtccctcga acgttccccc ttcgacgaac
    15721 gcctctggat gcacgaccag caccacgtcg tcggcgtagt agcccatggc gcgcgcgaag
    15781 tcgcgctcgt tggtggccgc gtactggtcg agaactaccc gcacgtcgtc ctgggacatg
    15841 cgggcagtat cgcgatcaga tcggctcgag cctgaacgtc gggtggtccg ccggattcgg
    15901 gaactgctcg aagtagtccc gagcgagcct gccgagcgcg ttgcggtacg tggtcacgac
    15961 acgctcgcgc tcacgtccgt cgacctcaac ggcgcggaag gccttgttgc cgagccagcc
    16021 gatgaactcg ccgcggccca cggcgcggag cgtgcgcgcc cagcgggtct ccgcacggtt
    16081 ggcgaccacg tacgccgcac cctcgtactc gagcggtgta accccgacga ggctcgacgc
    16141 gaccgcctcg cgatcgtcgt cgaccggctg gccgggaacc agccggagtt tcatgatcac
    16201 cggatcgagg atccggcttc ggagtgatgt ctcgggcatg atcaaccttc ctcgagtacg
    16261 ggcttggggg gacgagcgga tggctcttcg atcgccagcc gggggagccg gcgatgcagc
    16321 cagtccggga acttccaggt gttgcgtccg agcagttcca gcacggacgg cagcaacagg
    16381 cttctgatca cgaacgcgtc caagaacacg gcgctcgcga gactgaggcc gaacagcttg
    16441 atatcgcggt ccgggccgaa cacgaacgac aggaagacgc agaccatgat cgtggcggcg
    16501 gccgtgatca cacgccccgt ggaggccatg ccgttgacca ccgcgcgcga ggcgtcgcgg
    16561 cgcttcgtcc actcctcatg gatccgtgag acgaggaaca cctcatagtc cattgacagt
    16621 ccgaacacga tcgcgaacag gatcaccggc agccatggct cgatcggccc tggctgcacg
    16681 ccgaacagtc cgccgagcca gccgtgctgg aagatcgcga cggtcacgcc gagcgcggcc
    16741 ccgatcgaca gcaggttcat gagcgccgcc tggaccggga tcaccagcga gcggaacacc
    16801 acgagcagca gcagggcggc caagccgacg acgatcgcga cgaacagcgg cagcttgctc
    16861 gacagcacgt tcgtgaagtc gatgccggtc gcggtcaccc cgcccaccat cagcgtggtg
    16921 cctgtgctgt gggcgaccgg tggcaacacg tcgtcgcgca gcgacttgac gagatccgac
    16981 gtggccgtcg actgcggcga cgagcgcggg aacgcctcga tcacggctgt gcggccgttc
    17041 gggctgatct gcgcgggcgc caccgacgcc acgtccgggt tgctccgcac ggtcgcggag
    17101 atgcgctgga gcgccgcctg atcgtttttg ctcggcaggc tcgcgaccac gagcagcggc
    17161 ccgttgaagc ccttgccgaa gccggcggcg agcaggtcgt aggcctgccg cgtcgtctcg
    17221 ctcttcggag cgttgccctg atcgctcagg cccatccgca tgccgagcgc gggcgccgcg
    17281 atcaccagca tgatgaggag cccggcgatc gccgcgggcc atggcctgcg gcggatcaac
    17341 tccgcccagc ggtgccagaa cccggccgcc gcggcgtccg acacgactgt cgaaggagcc
    17401 cgtccgcgct ctgcgatccg gcgtccggcg cgggcgagga atgccggcag cagcgtgagc
    17461 gcggcaagca tcgtgaggag cacggcgatc gccgacgaca ccgcgagccc ggtgaggaag
    17521 gtgacgccca gcgccagcat cccgagcagc gcgaggatca cggtcgcgcc ggcgaacagc
    17581 accgcgcggc ccgcggtgtc catcgcctgc gagatcgcgg cctgcacatc gccgccgctg
    17641 cggtagctct cccggaagcg cgtgacgatg aacagcgcgt agtcgatccc gacgcctaga
    17701 ccgatcatcg ccgcaagctc ggtggagaag tccggcatgc tcagcaccct cgagccgagc
    17761 gcgatcagcc cgaacgcact gccgagtccg agcagcgccg taacgatcgg cagtcccgct
    17821 gcgaggaacg agccgaacgt gatcaggagc acgatgatcg cggcaccgag cccgaccgcg
    17881 ctcgctgtcc cgaacgagac gccctgagcc tgctcgatcg cctggccgcc gagctccacc
    17941 tgcagctgcg gcgagcgcgc gccctccgcg gtcgatatga cccgcttagt cgccgccttc
    18001 ggcagcgtgt tggcgcgctc gtcgaaggtg acggtcgcga aagcggtgcg gccgtcgcgc
    18061 gagatcgcct ccgcaccggc gcgcgtgaac gggctcacaa ccccggtcac gtgcggcagc
    18121 tttgcgacct tcgcgagcat cggggcgatg tgagcgcgta ccgccgggtc cgtgatccgg
    18181 ccctcgcgaa cgtgcagaac gatgcggtcg gagtcgccgg cctgcgacgg gaaatcgcgc
    18241 tgcagcagat ccgtcgccgc ctgcgactgc gtcccaggca gcgagttgtt gttcgagtag
    18301 tgggacccga ccgcgccaga gaccgcgagc gtgacgatca ggatgaccag ccagccgacg
    18361 accgtggcgc gccggtgcgt catggtccag ccggcgatac gagctacgag gcctcgcgcc
    18421 tgaggggcgg cgtggggcga gtccattgcg gtcctcctaa gggggagata aacttcagga
    18481 gtacaatcgg ttttgaagca aaagcttttg aaaccgaagg gtaaccgagg ggtgtgatgg
    18541 ctgtcaagac ggaagcgaag ggttcgcgag tggggctcgt ggatgccatc gcggggcagg
    18601 agccgatctc cgacgacctc cgcgagctgt tgggaatggt tcgccccgtc ttcatgggga
    18661 tgaagcgcgg cggcccggtg ccggcgccgc tcgagaacgc gttcgaggag gcgggactcg
    18721 gcccacgcca cgtggcgccg ctcatggccg tcaccctcga gggctcgctg agcgtcagcg
    18781 agctcgcggc acaactcggg ctgagcctct ccaccaccag cctgatggtg ggtgagctga
    18841 gccgcgccgg tctgctcgag cgcaaggagt gcgaggacga tcggcgtcgc accctcgtcc
    18901 acgtgaacga gaagtacgag gaatcgcttc ggaactggct ggccgaacgc atggagccgt
    18961 tccggcgcac gctcgagcgg ctctctccac gcgcgcgagc ggggttcctc gagggctggc
    19021 gcgtgctggc tgaggagacg gcgcgcatcg gcggcgagtc cgaggcgggc gccgacgcga
    19081 aggcgtaggc gcggctctac gcggcgatca ccagacgaac gagtcgatca ccttcgcgac
    19141 ctcgtcgcct ggaaccggac caacgcgaat ctcccaggcg ccgtcgatcg tttgcctgag
    19201 cttcccgtgg atcccggcgt cggatagaag ccgtgcgacc acggtggcgc ggcggttgcc
    19261 ggccgccccg ccccagcgaa cgtcgatccc gaaggagtca tcctcgacag gccagatcgt
    19321 cagtcggttt ggccgggtcg gcccgagctc agttcccatg agcgagatat cggcgcccgt
    19381 agccccagcc ttttagctgc ctggcgtcgc tagggggtcg gcggccggta agccggccgc
    19441 cccgtgagcg ccgcgccgag caccagcgtg tggatctccg ccgtgccctc gtacgtcacg
    19501 acggactcga ggttgttcgc gtgccgcatc acggggtact cgagcgtcac gccgttcgcg
    19561 cccaacagct ggcgcgcctc gcgcgcgacc tcgagcgccg cgcccacgtt gttcagcttg
    19621 ccgagactca cttgctcggg ccgcagccgg ccctcgtcct tcatgcgccc gagctgcagc
    19681 gccagcagct gcgcggtggt gaccttgcgc agcatctcgg cgagccgccg ctgggtgagc
    19741 tgcagcgccg caatcggcgc gccgaactgc tcgcgtgtga cgctgtactc gactgctgcc
    19801 tcgaggcacg ctcgagccgc cccggccgaa ccccacacga tcccgtagcg agcctcgttc
    19861 aggcacgaca gcggcgcgcc gagcccggtg gcctcgggca gctgcgcgtc cgcgggcacg
    19921 cggcagtcgt cgaagatcag ttcggaggtg acggacgcgc gcagcgagag cttgcggtgg
    19981 atgtcctgcg tcgtgaagcc gggcgtgccc tgctccacga tgaagccgcg gatgccgtca
    20041 tccgtgcgcg cccagacgac ggcgacatcc gacatcgacc cgttcgtgat ccacatcttc
    20101 gcgccgttca gcacccaatt gctgccatct cggcgcgcgc gtgtgcgcat cgacgccggg
    20161 tcgctgcccg cgtcggcctc ggtcaggccg aagcagccga cggcctcgcc cgccgccatc
    20221 cgcggcagcc agtgctgctt ctgctcctcg gagccgtagc gccatatcgc gtacatcgcg
    20281 agcgagccct gcaccgacac gaggctgcgc agcccgctgt cgcccgcctc gagctcctgg
    20341 caggcgaggc cgtagagggt cgcgctcgcg ccggccgttc cgtagccctc caggtgcata
    20401 ccgaggacgc cgagcttgcc cagctcggcc acgatctcgc gcggcagcgt cccctcctcg
    20461 aaccagtcgg gcacgtgcgg cagcagctcc gcctgcacga acgcgcggac ggtgtcgcga
    20521 aacaggcgtt cctcgtcgct cagcgaggcg tcgaggtcga ggaagtcacc ggccgtcatt
    20581 gcgggccggg tgccggttgc gctcatatct tctctccgtt ttcgtcgtgg ggaattccga
    20641 gctcgcgcag gacctcggcc gtgtgctccc cgagcagcgg cggcgccgag tcgcgcgaat
    20701 ccggcgtctc gtgaagccgg atcgggctgc gcgccagcct cagcgtacct gccgtcggat
    20761 gctcgacctc caccgtcgcc gggtcgcctg ctgcctcggc cgcgtcgaag gcctcaccca
    20821 cgccgcgaac cttccccgcc gacacgccgg ccgcctcgag gagctcgagc cactcgtcgg
    20881 cggttcgcgc gcgaaacgcc tccgtgagca acgggacgag ctcctcgcgc gcgcgcacgc
    20941 gaagctcgtt ggtcgcgaag cgggcgtcgt cggcgagctc aggccgctcg attgcctcgc
    21001 acagccgccg ccacagcccg tcgttcggcg ccgcgaccgc gagccagccc tcgcgagtct
    21061 cgaacggctc gtacgggacg atgctcgggt gcgcgttgcc gtagcgacgc gcctcctcgc
    21121 cggtggcgag cacgccctgc gccacgttca cgagcgacgc gaggccggcg tcgataagcg
    21181 acacctcgac gtgctcgccc acgcccgtct gctcggcgcg gcgcagcgcg gcgaggattc
    21241 cgacggccgc gtggagcccg cacagcacgt ccacgagcgc aacgcccacc ttgctcggct
    21301 cgccatcgag cggtccggtg acgctcatca gcccggtctc cgcctgggcg acgaagtcgt
    21361 agcccggccg cccttccggc gcgcgagcgc tgccgaagcc ggtgatcgag cagtacacga
    21421 gcgcgggatt gagctcacgc agctgctcgt acccgagccc gagccgctcc gccgtgccct
    21481 ggcggaagtt ctcgatcacc acgtgtgagc cctccgcccg tcgccgcacc gccgcgaggt
    21541 cgtcctcgtc cttgaggtcg agcgccgcgc tccgcttgtt gcgattcacc gacaggtagt
    21601 acgttgcctc gccgccggcg aacggcggtc cccaggaacg cgtctcgtcg ccgccgaccg
    21661 ggtgctcgac cttgatcacg tcggcaccca gatcggcgag cagcatcgtg cagtaggggc
    21721 cggcgagcac gcgcgacagg tcgagcacgc ggatgtggcc gagcgccttc ggcagcgcac
    21781 tggaaggaat accggattgc gtcatgtcat acctggtggg ttgtctggac aagctgcgga
    21841 ttatagctgg ccgcgcgcga tggtctggac cgaacgcgtg gtgggggcga gggccgcgtc
    21901 gatcacggcg agggcctgct gt
//
LOCUS       DASVXS010000151        42204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_40416, whole genome shotgun sequence.
ACCESSION   DASVXS010000151 DASVXS010000000
VERSION     DASVXS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 42204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42204
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40416"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VF752_08250"
     CDS             complement(<1..1111)
                     /locus_tag="VF752_08250"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX8855580.1"
                     /translation="MSEFADHVAFPRGKGVLADAPHAGAAGGAACGDLVRIAVRVEDG
                     RVAEAGFDASGCAAATAAGSAAVELIEGEPFLSAARVSAADISDALGELSNERRHAAE
                     LAADALHRALGAAAKDGAATATRSERRTLVAMSGGVDSAVAAQLALDRGDETIGVTLE
                     LWADPGTDGTKSCCSPYAVTGARALAHRMGIPHITLDLRDEFRREVVDDFLNAYANGG
                     TPNPCVRCNGLVRFDAMLVLAEKLGAARLATGHYARIARTPEGPLLKAAADANKDQSY
                     MLARVRPDELERLWFPLAELEKPRVRELAATASLPVARKPESQDLCFLAGTRREDFLA
                     RHGGPPAGEGELVSTDGGVIGTHSGQEGFTVGQRKG"
     gene            complement(1320..3125)
                     /gene="aspS"
                     /locus_tag="VF752_08255"
     CDS             complement(1320..3125)
                     /gene="aspS"
                     /locus_tag="VF752_08255"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX8855581.1"
                     /translation="MKPPPPNRYRTAWAGDQRAGNVGSDIRVAGWVHRRRDHGGLIFI
                     DLRDRTGLLQLVFRPEEAPDAHEVAHTLRPEDVITASGQLVNREEGTVNPNLPTGEVE
                     VNVAAVEKLADAETPPFQIDEEREVDETLRLKYRYLDLRRERMRRNIELRHDIVAAIR
                     DYFNPLGFLEIETPFLTRSTPEGARDFLVPFRLSPGNFFALPQSPQLFKQLLMVGGME
                     RYYQIVRCFRDEDARADRLPEFTQLDVEMSFVEEDDVITAMEGVTQAMLAAGGIEIEL
                     PLERLSYDDVMLRYGNDRPDRRPGMEIHDLADAFRESDFNVFSGTLEKGGVVRGFAAE
                     GEFTRRRFDELTEQAKRLGAGGLVWAVVEPDGWRSPIAKFLKREEMDKAARELGAEEG
                     DAIFIVADTAEVASRVLGTLRLEVAPPVEGHDIFWVVDFPMFEWNEDEQRFDALHHPF
                     TGPTGDLDGDPATWRSRAYDIVLDGNEIGGGSIRINRQDVQQKVFAALGISPEEARER
                     FGFLLDALKYGAPPHGGIAFGIDRIVALCAQQESIREVIAFPKMASGFDPLTGAPAPV
                     DEPQLRELGLKLIAPPAPGGPPPINPDEPGSADHN"
     gene            complement(3122..4369)
                     /gene="hisS"
                     /locus_tag="VF752_08260"
     CDS             complement(3122..4369)
                     /gene="hisS"
                     /locus_tag="VF752_08260"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX8855582.1"
                     /translation="MAGPKIQAPRGTFDVLPADGLRRERLRILSENLLGAAGYEPFET
                     PIFEATEVFARGVGESTDIVQKEMFTFEDQGGRSLTLRPEGTAPIARAYVEHGMHKLP
                     QPVKLRYWGPFFRHEAPQAGRYRQFNQVGIEALGSDDASLDAEAVLLLEELLRAAGAA
                     GVRLRLSSLGTAETRREYGEELRAYLRAHESELSEEVRARIDANPLRAFDSDDRGTRA
                     VMAEAPTLLDRLSDEDAQHFAEVRALLDDAGVAYELDGTLVRGLDYYTRTVFEFTSDE
                     LGAQSGVGGGGRYNGLVQLLGGPDTPGVGWAAGIERILLAAGEHVAVEEETDVYVARE
                     DGADSTAVFRVLRELRSEGLRAQAEQAGRSIKGQLKHADRIGARSVVIVAGDELRVRD
                     MKSGEQRAAGDVADAVRLVKESR"
     gene            complement(4372..5010)
                     /locus_tag="VF752_08265"
     CDS             complement(4372..5010)
                     /locus_tag="VF752_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8855583.1"
                     /translation="MDVRMFTVGPVQENTYLVRREGSDRAVIIDPGEEAPKLLAAIDS
                     LGVTLDGILLTHTHFDHVGAVAPVAKATGAEVWVPEIEKGVLADIMSYVPWPGFGPFE
                     SYDAEHTLKGGEKLEMADFEIHVIFTPGHSPGHVTFSIPDEKTILSGDVLFQGSVGRT
                     DLPGGDWNTLFESIRNLMDTLPEDTTVYPGHMGITSLGAERASNPFLGELAR"
     gene            complement(5012..5404)
                     /locus_tag="VF752_08270"
     CDS             complement(5012..5404)
                     /locus_tag="VF752_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855584.1"
                     /translation="MAQTRKKRRSKHRGTPAGTIERHGRTSKARGGTSSGSSSGSKTS
                     ARDRRLERMNKPPTWTGAFQRAGIAALLFIVLVVLIFGQAVSAALALGAFMLIFYVPA
                     GYYMDLFLYRRRMQKLGRPDETRRGKEA"
     gene            complement(5388..6272)
                     /locus_tag="VF752_08275"
     CDS             complement(5388..6272)
                     /locus_tag="VF752_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855585.1"
                     /translation="MLLTVPNISEGRDLEAIEALAQSFTPARLLDVHTDPDHGRSVFT
                     LAGRHGEIAAALVNGARAAAARIDLSSHDGLHPHVGALDVAPVVYLDDAERGAACAEA
                     LTAGALIGDELRIPVVLYGQLATSAGTRERADIRRGGPAELARRIEAGDLRPDYGPSH
                     AHPTAGAVLVTARPPLIAFNLDLATDDLELAKRIAARMRESGGGLPGVRAIGLPLAER
                     GRVQVSFNVHDHRAVPLRVLVEEVAREARIAEAELVGLAPRAAFDGFPDGVPLRGFDP
                     ERHLLEEALRSISSGSDT"
     gene            6303..6375
                     /locus_tag="VF752_08280"
     tRNA            6303..6375
                     /locus_tag="VF752_08280"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6336..6338,aa:Met,seq:cat)
     gene            6503..6625
                     /locus_tag="VF752_08285"
     CDS             6503..6625
                     /locus_tag="VF752_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855586.1"
                     /translation="MNNLAQHRRLLITLAVLIVIALAITLIAVYSGGGGGGGGY"
     gene            6963..7349
                     /locus_tag="VF752_08290"
     CDS             6963..7349
                     /locus_tag="VF752_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855587.1"
                     /translation="MQVGLQAYNRGDLEVVELAFHERVEFESPGGVGRLGFEASYRGR
                     EGHRAFEAEWRSGWAALRIEPREFIDLGEQVLLLAEIVGRGKGSGISVSQNIATLETL
                     GEDGKIVREQRYFDHSEALEAVGLLE"
     gene            complement(7375..8847)
                     /locus_tag="VF752_08295"
     CDS             complement(7375..8847)
                     /locus_tag="VF752_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855588.1"
                     /translation="MTATDTPRAKNLALLLLAMTQFMIVIDASIVNVALPSIGKALDF
                     SQDNLSWVVNAYVLTFGGFLLLGGRLADYLGRRRMFIYGLILFSIASLLGGLAQSEGW
                     LIAARALQGLGAAIVSPAALSIITTTFAEGAERNRALGVWGAVAGAGGAAGVLLGGVL
                     TQWAGWEWVLFVNTPIGLFVAFLAPRLLEESSVPEEARGSFDIPGAVSVTAGLALLVY
                     GFVQTPTDGWGSTATIARLAGAVFLIAIFLVIEMRERNPLMPFSIFRLRTLRGANVVG
                     LLIGMSLFSMFFFISLYLQQVLGFSALKAGVSYLPLAVTIIISAGIAGQLVTRVGFKP
                     TLVAGMLFVAAGLFWFSKVSAPGGSYTSDVLGPSLLAAVGLGLSFVPDTIAAVTGTKP
                     NEAGLASGLINTSQQVGGALGLAVLASVANSRTNDVFAAGEKSRIVGLTEGFQDAFLA
                     GTGIALLGAVLAAVLISSRDSKEHAEAARRGEAAAATEAG"
     gene            8945..9397
                     /locus_tag="VF752_08300"
     CDS             8945..9397
                     /locus_tag="VF752_08300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8855589.1"
                     /translation="MLHALSRDQLLPAEPGEVFDFFADAHNLEAITPPWLGFQVITPG
                     PVGMGVGTRIDYRLRLHGVPIHWRTRIEIWEPGRRFVDAQERGPYALWHHTHSFEKRG
                     GETLMRDVVRYAVPFGALGEVARRLLVERDLERIFDYRAAAVARNFTK"
     gene            9409..11175
                     /locus_tag="VF752_08305"
     CDS             9409..11175
                     /locus_tag="VF752_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855590.1"
                     /translation="MGTFRRLLGFLRPYRRGVIASLLLAAAAMVLGVLIPALVGKTVD
                     AVRNGHSNLWPYAAAILAAGVGRLVLSVFRRLVAGRVSLGVEFDLRNLMYRHLQSLEL
                     GFFDSQQTGQLMSRATVDLQSVRFFLGYGLVFIAQSLISILVAAVVMFAVNPGLAAVT
                     LAPTPFVIWVATQYGRKNRPASQEVQQRIAELTAEAEENISGVRVVKAFAQEERQFAR
                     FSRQVGRVFDQSMVSTRLRAFYNPFIGFLPNLGLAALLFVGGRQAINGTISVGEFVAF
                     YGYVALLTQPMRMLGVALGMSQRAVASGARVFEVLDREPRLESKPDAQPLPEGGGRVE
                     FRDVSFSYGGEDAEPVLDEIDLTVEAGSTVALVGATGSGKSTLVMLLPRLYDVTSGAL
                     LVDGADVRDVDLPELREQVAFVSDDPFLFSASVRENIAYARPDASFEQVQEAAERAGA
                     TEFIDELPEGFDTLIGERGYTLSGGQRQRIAIARALLRSPRLLILDDATSSVDATTES
                     RIARALGDLMRERTTFIIAHRLSTIALADEIVVLEEGRVLARGTHEELLEESELYREI
                     AEKGLPDQVFLTRDPLEREATL"
     gene            11175..12971
                     /locus_tag="VF752_08310"
     CDS             11175..12971
                     /locus_tag="VF752_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855591.1"
                     /translation="MRAWAARWWALFRRTELQRSRKLRGLLELLRPYRGRTVLMFVAL
                     LAATAASLAPPYLAGKAIDAGIRKGDVSALDWIVVAFLVSAIVTWTASYVQTYLVNWV
                     GERALQDLRLQLFAHLQRLSIGFYSRNKAGVVISRISNDVEALDQLVTDGVVTLFSSS
                     LTLIGTAVILFVLDWKLALITFVTFPILGIGSIVFRLVSAEAYRLTREKIAYVTAYLQ
                     ETLSGIRVVRAFGQEERHLSQFADLNEENRAANMKTVNLNAAYFPAVEFLSAVATAVI
                     LLYGGNQVLNGGDVTIGVLASFVFYLNSFFDPIQQLSQLYTTYQAGMAALDKIFELLD
                     EDPDLTDRPDAVELPTVRGEVRFEDVSFSYEGEGGEYALRDIDLVVPPGQTVALVGET
                     GAGKSTFAKLVARFYDPTSGRILVDGYDLRDVTERSLRSQLGVVPQEGFLFSGTIRDN
                     IAFGLRDASDDEVRAAARAVGADEFIERLPEGYDTEVGERGGHLSAGQRQLVAFARAA
                     AVDPRILILDEATSNVDVHTEGRIERGLRRLLAGRTSFVIAHRLSTIRGAGRILVMEH
                     GRIVEQGTHDELIELDGAYARLYQDWAEQAAA"
     gene            complement(13011..13081)
                     /locus_tag="VF752_08315"
     tRNA            complement(13011..13081)
                     /locus_tag="VF752_08315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13047..13049),aa:Gln,seq:ctg)
     gene            13184..13720
                     /locus_tag="VF752_08320"
     CDS             13184..13720
                     /locus_tag="VF752_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8855592.1"
                     /translation="MAELIASGLSRQAIARELGVAKSTVSYHARRLGEPGSPQFGRRY
                     DWAEIGRFYDAGHSVRECQERFGFSKQAWNNAVRRGDVVARPRLTPLAHLLAVDAPRS
                     RWNIKRRLLKEGLKQARCEQCGLERWRGEPLQLALHHVNGDPEDNRLENLALLCPNCH
                     SQTENFGVKNVAREALVG"
     gene            13725..13937
                     /locus_tag="VF752_08325"
     CDS             13725..13937
                     /locus_tag="VF752_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX8855593.1"
                     /translation="MPDIPIRGDSIRLGQLLKLAGLVEGGGEVKALLAEEPVLVNGER
                     ESRRGRQLHPGDVVQLGGEEIRVTSA"
     gene            complement(13944..15170)
                     /locus_tag="VF752_08330"
     CDS             complement(13944..15170)
                     /locus_tag="VF752_08330"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855594.1"
                     /translation="MAEREADYLLVGGGLAAANCARWLREEGADGSILLVGREPDPPY
                     SRPPLTKGYLRGKESRADALFRPDDWWQEQQIDLLTRTSVMKLDPGSRTAKLSSGDEV
                     RFAKALLATGANVRRLRVDGCDLDGIHYVRTFGNSDAIQADANDAERVVLIGGSYIGT
                     EAAASLTDLGKRCSIVMMEEVVLDRFYGPEVGGYFQRVLEERGVEVYGSEELDRFEGS
                     DGRVHKVVLKSGRELECDMVVIGIGVMPDVMLARQAGLELGENGGVKCSSRLETSAPG
                     IYAAGDICEYDSVIHGGPLRVEHWDVALSQGKTAALNMLGRDVPHDEVPYFFSDLADW
                     TGMEYVGPGSGDLVVRGSLDEGDFTAFWLDDETVKAAMAVGRSEDLEHARRFIKEKTP
                     VEPGALADGDTDLAAL"
     gene            complement(15175..15846)
                     /locus_tag="VF752_08335"
     CDS             complement(15175..15846)
                     /locus_tag="VF752_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX8855595.1"
                     /translation="MYIVVVGAGKVGWNLARELIGKGNEVTLIESDRARYQVVEEELE
                     HAVHYGDGSELWVLERAGIARADMVIAVTGDDEDNILICQVAREKYGVERVISRCNNP
                     RNLQHFELLGVKPAVSATDLILRLIEHEVPKYGFLHLLDLPQERLEIIEIEVAEGSPA
                     AGSQVQELGLPDGSLVISILRDGTGFVPLADSVIEAGDEVLLVLDTGLEQKIMERFRP
                     PVEVS"
     gene            complement(15849..16274)
                     /locus_tag="VF752_08340"
     CDS             complement(15849..16274)
                     /locus_tag="VF752_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX8855596.1"
                     /translation="MFVLIVGCGRVGSAIARAMLAEGHEVSVIDEDPEALALLESGYT
                     DTWEDQGGMFTVGTALEIDALIEAGIERADAFVASTDGDNTNLVIAQIAQRRFNIPRV
                     VVRVLDPARAAWYAEQGLSTVSPTQKAIEMLELAVRENA"
     gene            complement(16314..18233)
                     /locus_tag="VF752_08345"
     CDS             complement(16314..18233)
                     /locus_tag="VF752_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8855597.1"
                     /translation="MRWRGVGSPALFAIALTSVGSAAYFVLGVVAGDALGMTPLVFLG
                     ASFFFVLTMMTYVEGNALHPERGGASTFARYAFNELWSFIAGWAIILDYLIVMAMASF
                     TVSHYLAGFWGQLDSSGLELVIAAGVIAWVAWWNIRGLSPMSLQFLLRLGIANLVLLA
                     VVVIAGFANSSDFGLITDSIHLGSSPTWEDLIFASAVATVALTGIEAASGLASEIRVG
                     RRALRRLVFVGGISALIVLVMISIVALLNVPVVNGETQLGTRYVEEPLLGVANSYDPD
                     WLMHALRYSLGAVGALVLMTAVNGQMLGFARLAYSLATNRQIPSDLGRLTARYSTPYV
                     AIIVASLMAFGLVFSHDLKFMAGIFAFGAMLAFLLAHLSIITLRFREPDLPRPYRIPW
                     SVRVRGADLPVPAVLGAILAGAGWVSVVILHSGARYVGFGWLAGGLLLYVIYRKTAGK
                     SLTRRFTIPPTALQDAPEVEYGSILVPVFGSDLDDDIIGTAGRLASEDGDPGEGGTVI
                     EALYVLEVPMSLPLDARMPEDRIKAARKALSRAKEVGEEYEGVEVATAVVRARSVGAG
                     IVDEARRRGVDVIVLAAESPSKVRGGALLGGRGGPRDKVVGDVTRYVFEKAPCRVILT
                     AAPEGEAGTRMGVAP"
     gene            complement(18230..18433)
                     /locus_tag="VF752_08350"
     CDS             complement(18230..18433)
                     /locus_tag="VF752_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855598.1"
                     /translation="MQRAYRESTRVMGALIFVLGVVIVVVTLARGGGPLALGVVLGVL
                     FALLGAARFYLATRQEPRSRPPT"
     gene            complement(18491..20374)
                     /locus_tag="VF752_08355"
     CDS             complement(18491..20374)
                     /locus_tag="VF752_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8855599.1"
                     /translation="MAERRPKNQGLERVLGTGALFSTAYGNVGSSIYYALGLVASFAL
                     GMTPVVFIISGLIFAATAATYAEATAMYPEAGGSSSFARHAFNEFWSFFAAWGQMLNY
                     IITVAISAFFVPHYLGVFWEPLRSSPGDIFFGIGVVVLLGLLNIVGVREAAGLNVFLA
                     IADFITQLLLVIVGFVLVFSPSTLVNNIDFGTYPTVTDFLIAIPVGMVAYTGIETISN
                     MAEEARDYGHTIPRSIGFVVAAVVAIYAFLPSIALSAMPVKNGQTLLALSEEKGGYAG
                     DPILGVVKNMDLGFLQHPAEIYVGILAATILFIATNAGLIGVSRLTYSMGQHRQLPER
                     LRQLHPRFRTPYIAIIMFGAVACLTIIPGKADFLGQIYAFGAMLSFTIAHAAVIALRI
                     NQPDVERPWRGPGQIRVAGRNLPFFAVAGGLGTASAWVVVTVLNASTAIAGAVWLTIG
                     ISTYVIYRRRQGLSLTQTTKIVLPQPIVEHEVEYESILVAFEDDQYEPEAVATAAKLA
                     ARRRRGIYVLVLITVPNSSPIDAELPEQEEVAQATIDSAHVLGGRRVTGRWEKVRPGE
                     AGRRIVDEARDIRARAIVMSVPRRRAGGTPFGRTIETVLAERPARVIIESGNGTHERR
                     LAA"
     gene            complement(20566..22635)
                     /locus_tag="VF752_08360"
     CDS             complement(20566..22635)
                     /locus_tag="VF752_08360"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001951.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX8855600.1"
                     /translation="MSTVDWGIIGALGCAGAAVVYGLTTSRWLLAMSPGTETMANISR
                     AVQQGAEAYLKRQYQIIAGVAVVIAIVLAVAIDGRTAIGFVIGGTLSGAAGFIGMNVS
                     VRANARVAERARSGVSAALEVAFRGGAVTGLLVAGLALLGVAGYYGVLLLAGESDKTA
                     VDALVGLGFGGSLISVFARLGGGIFTKAADVGADLVGKIEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTAVAVMLLGVLTFTNQRDAAVFPLVVGGVSLLASIVGT
                     FFVRSRAGNVERALYQGLIASAVIAAVAFIPVTQWLMDDLRVRPDAPSLLHGAIGAGP
                     WDYWLCTLIGLAVTAGLFVITDYYTSTRFRPVRTTARASQTGHATNIIQGLAQGFQST
                     AAPAILIALGILGANELAGIYGVGVAVMAQLSLTGLIVALDAFGPITDNAGGIAEMAD
                     LPEEVRNVTDPLDAVGNTTKAVTKGYAIGSAVLAALVLFAAFIIELQDEAGSKPLKSF
                     DISQPEVLIGLLLGGMMVYLFSALAIEAVGRAGGGVVEEVRRQFREKPGIMEGTDTPD
                     YARAVSIVTAAAQREMIVPALIPILIPLIVGLLSYQALGGLLIGVIVVGFFAAVSMTS
                     GGGAWDNAKKLIEDGEFGGKGSEAHAASVTGDTVGDPYKDTAGPAINPMIKVANIVAI
                     LIIPFLV"
     gene            complement(22706..23515)
                     /locus_tag="VF752_08365"
     CDS             complement(22706..23515)
                     /locus_tag="VF752_08365"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX8855601.1"
                     /translation="MSPEARPHVGIFGGSGFYEFLDDTEAVTVETPYGPHSAPIRIGH
                     IDGTAVAFMPRHGDKHELPPHRIDYRANLWAMKEVGVRRLIGPSACGSLKPELQPGTF
                     VVVDQFVDRTSGRRDTFYDGPGTTHASAAEPYCPELRGILVDCARRLDIPVVDGGTVV
                     VIQGPRFSTRAESRWFAAAGWDVVNMTQYPEAWLARELELCYANVSLVTDYDVGLEGS
                     PHVEPVSAEMALRVFAENLAKLRDLLFQAVPRIGPQPEDICATALQSAIAH"
     gene            complement(23512..23928)
                     /locus_tag="VF752_08370"
     CDS             complement(23512..23928)
                     /locus_tag="VF752_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855602.1"
                     /translation="MTQEGQEGGQRQPTEEELRAALEEQMRKITVRDVVLQTVVSLVN
                     LSGRRLGLTGAPEEKDLEQARLGIDAVRALLPLLPDDEMAPVRDALSQLQVAYAREAG
                     GGAGQEQPAEDQPSEPSAEDQERAKARSKIWTPPGT"
     gene            complement(23925..25070)
                     /locus_tag="VF752_08375"
     CDS             complement(23925..25070)
                     /locus_tag="VF752_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX8855603.1"
                     /translation="MHAAALREVFEASEDFTVGLEEEFGILDPGSRDLAQRFEELRQA
                     AQQDDVLADSIAGELISSEIEIRSGRGQTLADALAAQQEARRRLFRLAGDHGVLLCAT
                     GTHPWADYREQHIIDTEHYRRVEEGLKYVAWRNNTFSTHVHVGVRDADRAVAVCDRLR
                     PVLPELLAISANSIFLEGRDTGLHTVRTQIFTKSFPRCGIPDHYGTAAVYEEYIDMLY
                     RTGSIVEDTQIWWSIRPHRSFGTVEVRICDAQSTAEESSALEALIAACLAQAVMDHDE
                     SVPYEPKPRRLIEENFWRAIRFGLDGKMLDLDRLEEFPAAALPDRLLAWTAPARALLG
                     IDVQVPAENGAQRQRRALRSGASMEEVFAAEVEATQRTYAAAEGVTT"
     gene            complement(25086..25766)
                     /locus_tag="VF752_08380"
     CDS             complement(25086..25766)
                     /locus_tag="VF752_08380"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX8855604.1"
                     /translation="MTINQLYDGSTDVAEGTGDRLSLGVAARLSRYLQVLTQAKKMGK
                     ETISSQELADYTHVNSTQIRRDLSGFGKFGKRGVGYNVDSLVSQIRKILRTAGQHNIA
                     LFGAGHLGQAIASSDIFADHGFRVVAIFDNSERKIGQQVGEGKTALLVRPTDDLDKVV
                     QEEDIVVGVIAVPTEAAQAVADDLVEAGVKIIFNYSEALLQVPPEVTVHTSSPAVDLL
                     YALYFYLT"
     gene            complement(25763..26008)
                     /locus_tag="VF752_08385"
     CDS             complement(25763..26008)
                     /locus_tag="VF752_08385"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX8855605.1"
                     /translation="MRLVTLYGKPGCCLCDDAREVVLSVRQSTPFALEEVDVSLDSAL
                     HRQYGERIPVVAVDGEELFEYHVDPDALADSVSRVET"
     gene            complement(26005..26988)
                     /gene="tsaD"
                     /locus_tag="VF752_08390"
     CDS             complement(26005..26988)
                     /gene="tsaD"
                     /locus_tag="VF752_08390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX8855606.1"
                     /translation="MILGIETSCDDTCAAVVSHAGEVRSNVVASQGLLHERYGGVVPE
                     IASRRHLELIDAVIDDALERAGATLDGADTIAVTQGPGLIGALLVGVSEAKAIAAARR
                     LPLAPVDHLHGHVVASTLGDDPVEPPYLCLVASGGHTFIARVDELSSHRRLGTTLDDA
                     AGEAFDKGARLLGLGYPGGPAIDRLAREGDPTAFDFPRSAPGELDFSFSGLKTSLLYA
                     VRDLGETETERRKADLAASYQRAIVDALVARAKKAIEREGLERLAIGGGVAANRELRE
                     RVAQLGLKVWIPDFSLCTDNAAMIAGAARFQEPLPFPGYLALDASARPVAA"
     gene            complement(26985..27488)
                     /gene="rimI"
                     /locus_tag="VF752_08395"
     CDS             complement(26985..27488)
                     /gene="rimI"
                     /locus_tag="VF752_08395"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX8855607.1"
                     /translation="MTPPLEIRRLSYSDLPQVIAIERRAFPTAWSLAMFVLELSKPSG
                     ICLAATIDGKIAGYLVCSRYDTVWHLMNVAVDDRRRRTGIATALLNRLFETADRPGEQ
                     YTLEVRESNTSAIALYERFGFRAAGKRRGYYHDNKEDALIMWRTADASGPVTDPAREA
                     SERAAPA"
     gene            complement(27485..28165)
                     /gene="tsaB"
                     /locus_tag="VF752_08400"
     CDS             complement(27485..28165)
                     /gene="tsaB"
                     /locus_tag="VF752_08400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX8855608.1"
                     /translation="MNLLGFDTSTAATSACVLRDDGAGFEFVPPVEALGEPPAHAREL
                     LPEVTGCLERAGLSFEQLDAVAVGVGPGTFTGLRIGVATARGLAHAHGLEVRPVSSLA
                     ALAEGIEAPRRLALIDAKRGELFAALYDDGRERIAPFVATPEALIERLRSDGLLSDES
                     LLAAGDGSLRFREALEAGGVAVAEPDSTSHVVRALHLCRLAGQTFAVPADAVLPQYLR
                     EPDAKPPS"
     gene            complement(28162..28575)
                     /gene="tsaE"
                     /locus_tag="VF752_08405"
     CDS             complement(28162..28575)
                     /gene="tsaE"
                     /locus_tag="VF752_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX8855609.1"
                     /translation="MRIETASPEETERAGSRLAERLKPGDVVLVSGELGTGKTTFVRG
                     AARALGVREPVTSPTFTIGQLYDGRPEVAHLDLYRLSTLEGEDPALLDDYVTPERIAF
                     LEWPDVALPRLDSVRARVRIEHLGGDRRALELPDE"
     gene            complement(28601..29287)
                     /locus_tag="VF752_08410"
     CDS             complement(28601..29287)
                     /locus_tag="VF752_08410"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8855610.1"
                     /translation="MGRAAEERRAELIKLYRELQDCHDCPLAATRTNVVFGSGNADAD
                     LMFVGEAPGFHEDQQGKPFVGAAGKLLDKLLAEIGLEREQDAFIANVLMCRPPGNRDP
                     EPEEIEECKPYLMQKIRLIEPRVICTLGNFATKCITGRPEGITRAHGRPQERVIEGLP
                     LVIFPLFHPAAGLRSGAVLTQLRDDFQKLPELIATARPKAAAPPAPPAAGPPEPAMMA
                     AEPPQLGLFG"
     gene            29443..30225
                     /locus_tag="VF752_08415"
     CDS             29443..30225
                     /locus_tag="VF752_08415"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX8855611.1"
                     /translation="MSAQATHAQLAQHGLRSIAVVGRGRLGTAIAPALRNAGFDVIGP
                     LGRGERAAADAVLLCVPDSQIAAAAAAITGCAPLVGHTSGATPLSALEPARGERFGLH
                     PLQTFTGGDGPESFTGVGCAVGGSSERALDAAMEIAGALGMRPFELADEQRAAYHAAA
                     SIASNFLVALESAAETVAGSAGLPPERTRELLAPLVRATVENWADLGPERALTGPVAR
                     GDELTVAKQRTALREHTPELLPLFDALVAQARAVAGRGEAAA"
     gene            30222..31067
                     /gene="panC"
                     /locus_tag="VF752_08420"
     CDS             30222..31067
                     /gene="panC"
                     /locus_tag="VF752_08420"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX8855612.1"
                     /translation="MKTVRTIAELREAVRAAERPVGLVPTMGALHEGHLSLIRRAREQ
                     SATVVVSVFVNPSQFRPGEDFEAYPRNEQRDAEVAEREGAGIVFAPSVEEVYPPGFDT
                     SVQVGALTEVLDGHPAQRGPEHFHGVTTVVTKLFNMVQPDAAYFGQKDAQQAIVLRKL
                     ARDLDMPVRIEVCPTVREPDGLALSSRNVYLGPEERERALALSRALRAAEQAVAAGTV
                     EADVVLAAARTELEAAGIEPDYLELRSTNDLSPVERVNGSTLLAVAARVGRARLIDNT
                     VLGGA"
     gene            31064..31444
                     /gene="panD"
                     /locus_tag="VF752_08425"
     CDS             31064..31444
                     /gene="panD"
                     /locus_tag="VF752_08425"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX8855613.1"
                     /translation="MKRTMLKSKIHRGRITASDLHYVGSITIDAELLEAADILEHELV
                     HVLDVDNGARFETYTIAGARGSGEIKVNGAAARLVHTGDTVIVVSYASYDERELESYE
                     PKVVHVNAHNEIIQVDDAVAELVA"
     gene            31441..32346
                     /gene="panB"
                     /locus_tag="VF752_08430"
     CDS             31441..32346
                     /gene="panB"
                     /locus_tag="VF752_08430"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX8855614.1"
                     /translation="MSARPNVSPAPPAKRKATTLSTLAEKKALGEPIVMVTAYDYPSA
                     QVAEEAAVDLVLVGDSGAMTVLGYPSTVPVSIDEMLMLCAAARRGLSTPMLIGDLPFG
                     SYEVSNEQAIATAQRFVKEAGCDAVKLERGGVSAERAKAIVDAGIPVMGHVGLTPQTA
                     TALGGYRSQGRTAERALEVLDDALALQEAGCFAIVFEAVPSEFTELVMPRLAIPVIGI
                     GAGPSTDGQVLVFHDLLGIYDGHAARFVKRYGNVRQAMIDGVSAFAAEVRERRYPEPD
                     HGYTMAPDEVARLKDALTHSERTKA"
     gene            complement(32405..32908)
                     /locus_tag="VF752_08435"
     CDS             complement(32405..32908)
                     /locus_tag="VF752_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX8855615.1"
                     /translation="MQTKRYAGLLAAGVAGVLLVAGCGGGEKRSSGTGTTPSGTSTQA
                     APTGKASATVNVTETDFKLNPSNPSVKPGVVEFKVKNSGQTVHSLEVEGPNGEVRLPS
                     QLNPGDSSELKADLAKPGKYEWYCPVDNHKQMGMKGTITVGSSGSAGSSTTDNGSTDT
                     TGSSRGY"
     gene            complement(32966..33580)
                     /locus_tag="VF752_08440"
     CDS             complement(32966..33580)
                     /locus_tag="VF752_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX8855616.1"
                     /translation="MGDLYRKAVLWERKWRWYERNALPWNRARIHWHALQREAFIRFP
                     VQGNVLECLDDGRLEIGANTLLEPGCWITIGEGARVRIGEGCFLNIGTMLAAIDSIEI
                     GDHTMFANHCFVGDSAHRFDDPTRPITWQGFTSKGPVRIGSNCWFGVNCVVTSGVTVG
                     DRCVIGANSVVTSDLPDGVVAAGSPAKVIKEIEFAKPEDLPAAG"
     gene            complement(33756..34979)
                     /locus_tag="VF752_08445"
     CDS             complement(33756..34979)
                     /locus_tag="VF752_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8855617.1"
                     /translation="MSSVRHHFALRAHPGVLLLVLAAALALAPWQAEAQSPNGRSSHT
                     GAEVTRLAHASESVLVLGSGYREGGSGEVRSVQRRLSRAGFDPGPIDGLYGLLTTRAV
                     TRFQQSQGIAVDGKVGPQTRRALRSPLLEPGSGYQQGGSGEVRTLQRELAHRGFQPGP
                     VDGLYGPQTTRAVVRFQQARHLAADGVAGPLTQAELPTAQRARTRSTRERAKPASGGG
                     HERAKPASRGGHERAKPTSGSSNERPKPAKAPPPPKTAPVEPSADHAGGGNENTFALL
                     FILAIIATAVALVWLGTRTRRRRSIPRRPRRTHRKRRRVGDRAIARLMRLGFRYDYVR
                     HAYVMTGLGARWGPVFEHWPEEEGGEGDQDGEPASATPIRPSPQPAESRQRSNRFRPP
                     PERRRQAARERGDRR"
     gene            complement(35114..36769)
                     /locus_tag="VF752_08450"
     CDS             complement(35114..36769)
                     /locus_tag="VF752_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855618.1"
                     /translation="MLAVALLGLGLLPSVALALDTGATVTNVVDGATATVNSTVDSVT
                     QAAPPATPSAPSAPSTDTPSAPSAPSTPSTNTPSTPSTPSAPSTPSAPSGNTTSVPSS
                     PDTSSVTNAVSDVTNTATSTVSDTTSAVTNTAASTSDSVSRTVDSGVTSAEQTIDSIG
                     DTVTSTTDSTSGSAGSTANSTADSVESTAGSTTGSGGPSGGSPASTLDSATAPLVDTT
                     TSLVDGTVAPVLNSTTSLVDGALAPVVDTTTSLVDEAVAPVVDTTTSLVDGAVAPLVD
                     TTTPLLDTAVVPLLDTTTSLVDGAVGPVLDSTTPLLDTTVVPALDSTTPPLDGAVSPL
                     LDSTTSLLGAMDTTTAPLLGTGVPPTDSLLAPLAPVVETTAPLLGTVLPGPPAPPPSA
                     LAPAIERTPAPTDPATPSVGQALTRVPLPQSLLSHTPSVLAPAGATRDERRAHGSVSP
                     AKLGRTAASMLAGATSPIAATAPSTRDAVSRASRPAHPDSPFPAGAAQGSGAASPSGL
                     MFGGIAAFLILFALTAPALRRRLSVDPACFRPVPFIALSERPG"
     gene            complement(37035..39938)
                     /gene="uvrA"
                     /locus_tag="VF752_08455"
     CDS             complement(37035..39938)
                     /gene="uvrA"
                     /locus_tag="VF752_08455"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX8855619.1"
                     /translation="MSQRSELVISGAREHNLKDLHLEIPRNALVVITGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWARVGHPHCYNCGQPIVAQSAEQIVDQVMTLQEGERFMVLAPVVRG
                     RKGEYGKLFEDLRAEGYARVKVDGELRRLEEDIALDKKFKHDIAVVVDRLVMRPDLRK
                     RLADSVETAVALAEGIVEIETVPSDGSEGVVHTYSERFACLNCGTSMPELEPRMFSFN
                     SPHGACPRCTGLGSQMEIDPDLVVPDPSLSLAEGALLPWSTTASNYYEQMTQAIADRY
                     EVDLETPWEDLPEEVQECFLYGTNGDRLYISYKNRYGRRRSYTTSFEGIITNLERRYH
                     ETDSDYSREKIEEYMTLRPCPECHGARLRPESLAVKVGGLGIHELTRMSARRAIEWFE
                     ALELSNTERQIARLILREIDERLRFLDNVGVGYLSLERAAATLSGGEAQRIRLATQIG
                     SSLVGVLYILDEPSIGLHQRDNERLIRTLERLRDLGNTVIVVEHDEGTMRAADHIVDL
                     GPGAGEHGGYLVAQGTPDDVMNVKESATGQFLAGSRTIEVPAKRRRPSGYIEIEGATQ
                     HNLRGINAKIPLGVFCCVTGVSGSGKSTLINDVLYKSVANRLHRAKLRPGAHRRVTGL
                     DQVDKIINIDQSPIGRTPRSNPATYTGVFDSIRELFSRTQEARARGYKPGRFSFNVKG
                     GRCEVCRGDGQIKIEMHFLPDVYVPCEQCHGRRYNRETLEVRFKSKTIADVLDMPVEE
                     ALDFFRHIPKIKRRLQALHDVGLDYIRLGQPATTLSGGEAQRVKLATELAKVATGRTL
                     YILDEPTTGLHFADVQRLLEMLERLVDQGNTVVVIEHNLDVIKTADRIVDMGPEGGDE
                     GGLVVAQGTPEEVAGTEGSHTGRFLAGLVTPKKRRAPSRNGGPKRRVAEPAAA"
     gene            40096..40761
                     /locus_tag="VF752_08460"
     CDS             40096..40761
                     /locus_tag="VF752_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8855620.1"
                     /translation="MTDRTRHDEITHSRAGDVFVNPVTGERAVIVCGTADSTDGSLSV
                     HLFVAPGGAVAAEHYHPQLVERFRVLSGHLAVSVEGRRSHMGPGDELSIPPGVIHDWW
                     NAGETEAEVLVEVTPGRRFELMVGSLFGLARDGKVNAKGMPNLLQLAVMASEFDDTVR
                     FVKPSPIVQKLLFAVLAPLGRLLGYRPYYAEYLVPAEHIEPERSVLDMVRSHHAAPRT
                     ALA"
     gene            40765..41340
                     /locus_tag="VF752_08465"
     CDS             40765..41340
                     /locus_tag="VF752_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8855621.1"
                     /translation="MLERVDTVVVGAGQAGLAASFRLERAGVDHVVLERGRVGESWLS
                     QRWDEFRLNTPNWANLLPGMDLRGADPHAFASAREFAARLARYVRVNRLPVLTGVSVM
                     GLEPDGEHGWRVTTDAGDYLCRDVVLAAGWLNRPRFPAVALTVPLSIERIHTAAYRNA
                     SQLPDGGVLVVGSGQSGVQIAEDLLGSGRSV"
     gene            41404..41661
                     /locus_tag="VF752_08470"
     CDS             41404..41661
                     /locus_tag="VF752_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855622.1"
                     /translation="MIWATGFSPDLRWLAPSLLEREGLHVLGRAWLRSRKSGMVYGAA
                     EDSAAIVARITGGRAVEMGRRRLAHQAPAAEHAVQQPQLVA"
     gene            41985..>42204
                     /locus_tag="VF752_08475"
     CDS             41985..>42204
                     /locus_tag="VF752_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX8855623.1"
                     /translation="MDLDRTRIERKDFSVVRRGYDPSEVDLHLRDLADAVAELRAELR
                     STPAGVAGAAAEHVKTIVEAAEKSAAEIE"
BASE COUNT         6414 a        14592 c        14713 g         6485 t
ORIGIN      
        1 tccccttgcg ctggcccacg gtgaagccct cctgcccgga atgcgtgccg atcacgcctc
       61 cgtcggtgct cacgagctcg ccctcgcctg ccggcgggcc gccgtgtcgt gcgaggaagt
      121 cctcgcgacg cgtgcccgca aggaagcaca ggtcctggct ctccggcttg cgcgccaccg
      181 gcaggctcgc ggtcgcggcg agctcgcgca cgcgcggctt ctcgagctcg gccagcggga
      241 accaaagccg ttcgagctcg tcgggcctca cgcgcgcgag catgtacgac tggtccttgt
      301 tggcgtccgc cgccgccttg agcagcggtc cctccggcgt ccgcgcgatg cgcgcgtagt
      361 ggccggtggc gagccgcgcg gcgcccaatt tctcggccag caccagcatc gcgtcgaagc
      421 gcaccaggcc gttgcagcgc acgcacgggt tcggcgtgcc gccattcgcg taggcgttca
      481 ggaagtcgtc gaccacctcg cgccggaact cgtcgcgcag gtcgagcgtg atgtgcggga
      541 tgcccatgcg gtgcgcgagc gcgcgcgccc cggtgacggc gtacggagag cagcagctct
      601 tcgtgccgtc ggttcccgga tccgcccaca gctcgagcgt taccccgatc gtctcgtcgc
      661 cgcggtcgag cgcgagctgc gccgccacgg cgctgtccac gcctccgctc atcgcgacca
      721 gggtgcggcg ctcgctgcgc gtcgctgtcg cggcaccatc cttggcggct gcgccgagtg
      781 cgcggtggag cgcgtcggcg gcgagctctg cggcgtgcct gcgctcgttc gacagctcgc
      841 cgagcgcgtc cgagatgtcg gcggccgaca cgcgtgctgc gctcaggaag ggctcgcctt
      901 cgatcagctc gaccgcagcg cttcccgccg cggtcgcggc agcgcagccg cttgcgtcga
      961 agcctgcctc ggccactcga ccgtcctcca cgcgcaccgc gatgcgcacg aggtcgccgc
     1021 aggcggctcc gccggcggcg ccggcgtgcg gtgcgtccgc cagcacgcct ttgccgcgcg
     1081 ggaacgccac atggtcagcg aactcgctca cgtgctcaag ggtcgcagaa gaaggttggc
     1141 cccagcctgc cgatgccgcc tcgggagttg aaccttgctt cagcaagatt gggcgcccgc
     1201 cagagaggac cacggccctc tcccgagtcg ggcacccgac tcccatggtg ttggttcaag
     1261 taacgagacg gctgtcgtac aggctcgagc cggattcgag cgtagcaatg atgcacgcgt
     1321 cagttgtggt ccgccgagcc tggttcgtcg gggttgatcg gcggcggtcc gcccggcgcc
     1381 ggtggcgcga tcagcttcag gccgagctcc cgcagctgcg gctcgtccac cggcgcgggc
     1441 gcacccgtca gcgggtcgaa gccgctcgcc atcttgggga acgcgatcac ctcgcggatc
     1501 gactcctgtt gcgcgcacag cgcgacgatc cggtcgatgc cgaacgcgat cccaccgtgc
     1561 ggcggggcgc cgtacttcag cgcgtcgagc aggaagccga agcgctcgcg cgcctcctcg
     1621 ggtgagatcc cgagcgcggc gaacaccttc tgctgcacgt cctggcggtt gatacggatc
     1681 gagccgccgc cgatctcgtt gccgtcgagc acgatgtcgt aggcgcggct gcgccaggtc
     1741 gccggatcgc cgtcgagatc gcccgtgggc ccggtgaacg gatgatgcag cgcgtcgaag
     1801 cgctgctcgt cctcgttcca ctcgaacatc gggaagtcga cgacccagaa gatgtcgtgc
     1861 ccttcgaccg gcggcgcgac ctcgagccgc agcgtgccga gcacgcggga cgcgacctcg
     1921 gccgtgtcgg cgacgatgaa gatggcgtcg ccctcttcgg cgccgagctc gcgcgcggcc
     1981 ttgtccatct cctcgcgctt caggaacttc gcgataggcg agcgccagcc gtcgggctcg
     2041 accacggccc acacgaggcc gcccgcgccg agtcgcttcg cctgctcggt cagctcgtcg
     2101 aagcgcctgc gcgtgaactc gccctcggcg gcgaatccgc gcaccacgcc gcccttctcg
     2161 agcgtgccgc tgaagacgtt gaagtccgac tcgcggaagg catcggccag gtcgtgaatc
     2221 tccatgccgg gccggcgatc ggggcggtcg ttgccgtagc ggagcatcac gtcgtcgtag
     2281 ctcaggcgct cgagcggcag ctcgatctcg attccgccgg cggcgagcat cgcctgagtc
     2341 acgccctcca tcgccgtgat cacgtcgtcc tcctcgacga acgacatctc cacatcgagc
     2401 tgcgtgaact cgggcaggcg gtcggcgcga gcgtcctcgt cgcggaaaca gcggacgatc
     2461 tggtagtagc gctccatgcc gcccaccatc agcagctgct tgaacagctg gggcgactgc
     2521 ggcagcgcga agaagttgcc tggcgagagc ctgaacggga caaggaagtc gcgggcgccc
     2581 tcgggcgtcg agcgcgtgag aaacggcgtc tcgatctcga ggaagccgag cgggttgaag
     2641 tagtcgcgga tggcggccac gatgtcgtgg cgcagctcga tgttgcggcg catgcgctcg
     2701 cgccgaaggt cgaggtagcg gtacttcaga cgaagcgtct cgtcgacctc gcgctcctcg
     2761 tcgatctgga acggcggcgt ctccgcgtcg gccagcttct cgacggcggc gacgttgacc
     2821 tccacctcac cggtcggcag gttggggttg accgtccctt cctcgcggtt cacgagctgg
     2881 ccgcttgcgg tgatcacgtc ttcagggcgc aacgtgtgcg cgacctcgtg ggcgtcgggc
     2941 gcctcctctg gtcggaagac gagctggagc agcccggtgc gatcgcgcag gtcgatgaag
     3001 atgaggccgc cgtggtcgcg ccggcggtgt acccaaccgg ccacgcgaat gtcgctgccc
     3061 acgttgccgg cgcgttggtc gccggcccag gcggtgcggt accggttcgg tggcggcggc
     3121 ttcaccgcga ctccttcacg agccgcacgg catccgccac gtcacccgcc gcgcgctgct
     3181 cgccgctctt catgtcgcgc acgcgcagct cgtcgcccgc cacgatcacg acggagcggg
     3241 cgccgatccg gtcggcgtgc ttgagctgac ccttgatcga tctgcccgcc tgctccgcct
     3301 gagcgcgaag tccctcggag cggagctccc ggagcacgcg gaagaccgcg gtggaatccg
     3361 caccgtcctc gcgcgcgacg tagacgtcgg tctcctcctc caccgcgacg tgctcgccgg
     3421 ccgccagcag aatgcgctcg atcccggccg cccagccgac cccgggcgtg tccggcccgc
     3481 cgagcagctg cacgaggccg ttgtagcggc cgcccccgcc cacgccgctc tgcgctccga
     3541 gctcgtcgct cgtgaactcg aacaccgtgc gcgtgtagta gtcgagcccg cgcacgagcg
     3601 tgccgtcgag ctcgtacgcc acgccggcgt catcgagcag cgcacgcacc tcggcgaagt
     3661 gttgcgcgtc ctcgtcggag agcctgtcga gcagagtcgg cgcctcggcc atgactgccc
     3721 tcgtgccccg atcgtcggaa tcgaaagccc gcaacggatt cgcgtcgatt cgagcacgca
     3781 cctcctcgga gagctcggac tcgtgcgcgc gtagatacgc gcgcagctcc tcgccgtact
     3841 cgcgccgcgt ctcagcggtg ccgaggctgg agaggcgcag ccggacgccg gccgcgcctg
     3901 ccgcgcgcag cagctcttcg agcagcagga ccgcctcggc gtcgagggag gcgtcgtcgg
     3961 agccgagcgc ctcgatcccg acctggttga actggcggta gcggccggcc tgcggcgcct
     4021 cgtggcgaaa gaaaggcccc cagtagcgga gcttcacggg ctgcggcagc ttgtgcatgc
     4081 cgtgctccac gtaggcgcgc gcgatcggcg cggtcccctc gggccggagc gtcagcgagc
     4141 gcccgccctg gtcctcgaag gtgaacatct ccttctggac gatgtcggtg ctctcaccta
     4201 ccccgcgcgc gaacacctcg gtcgcctcga agatcggcgt ctcgaacggc tcgtagcccg
     4261 cggcgccgag caggttctcc gagaggatgc gcagccgctc gcggcggagt ccgtccgcgg
     4321 gcagcacgtc gaaggtgccc cggggggcct ggatcttcgg gccggccacg actaccgggc
     4381 cagctcgcca aggaacggat tgctggcccg ctccgcgccc aggctcgtga tgcccatgtg
     4441 gcccgggtag acggtcgtgt cctccggcag tgtgtccatc aggttgcgga tcgactcgaa
     4501 cagcgtgttc cagtcgcccc ccggcaggtc ggtgcggccg acagagccct ggaacagcac
     4561 atcgcccgag aggatcgttt tctcgtcggg gatcgagaac gtgacgtgac ctgggctgtg
     4621 ccccggggtg aagatcacgt ggatctcgaa gtcggccatc tcgagcttct ccccgccttt
     4681 gagcgtgtgc tcggcgtcgt agctctcgaa gggcccaaat cccggccagg gcacgtagct
     4741 catgatgtcg gcgagcacgc ccttctcgat ctcgggaacc cagacctccg cgccggtggc
     4801 cttcgcgacc ggcgcgaccg cgccgacgtg gtcgaagtgc gtgtgagtga gcaggatgcc
     4861 gtcgagcgtc acccccagcg agtcgatcgc cgcgagcagc ttcggcgcct cctcgcctgg
     4921 atcgatgatc accgcgcgat cggagccctc gcggcggacc agataggtgt tctcctgcac
     4981 cggcccgacc gtgaacatgc gcacgtccac gtcaggcctc tttcccccgg cgcgtctcgt
     5041 ccggacggcc gagcttctgc atgcggcgac ggtagaggaa gagatccatg tagtaacccg
     5101 cgggcacgta gaagatgagc atgaaggcgc cgagcgcgag cgccgcgctc acggcctgtc
     5161 cgaagatcag gacgacgagc actatgaaca gcaacgccgc gatgcccgcg cgctgaaacg
     5221 cgccggtcca ggtgggcggc ttgttcatcc gctcgaggcg gcggtcgcgg gcgctcgtct
     5281 tgcttccgct cgaagagccg ctcgaggtgc caccgcgagc cttgctcgtg cggccatgcc
     5341 gctcgatcgt gccggccggc gtgccgcggt gcttgctgcg tcgcttctta cgtgtctgag
     5401 ccactgctaa tggagcgtaa cgcctcctcc aggagatgcc gctcgggatc gaagccgcgg
     5461 agcggcacgc cgtccgggaa gccgtcgaac gcggcgcgag gtgcgagccc gacgagctcg
     5521 gcctcggcga tccgcgcctc gcgcgccacc tcctcgacta gcacccggag cgggaccgcg
     5581 cgatggtcgt ggacgttgaa cgagacctgc acgcgaccgc gctcggcgag cggcaaccct
     5641 atggcccgca cgcccggcag gcctccgccc gactcgcgca tcctcgccgc gatgcgcttt
     5701 gcgagttcga ggtcgtctgt cgcgaggtcc aagttgaagg cgatgagcgg agggcgggcc
     5761 gtgacgagca ctgcgcccgc cgtcgggtga gcgtgcgagg ggccgtagtc gggccgcagg
     5821 tcgcccgcct cgatgcgccg ggcgagctcg gccggtccgc cgcggcgaat gtccgctcgc
     5881 tcccgcgtgc ccgcgctcgt cgcgagctgc ccgtatagga cgacagggat ccggagctcg
     5941 tcgccgatga gggcacccgc ggtgagcgcc tccgcgcacg cggcgccccg ctctgcatcg
     6001 tcgagataga cgacgggcgc cacgtcgagc gcgcccacgt gagggtgcag cccgtcgtgc
     6061 gagctcaagt cgatgcgggc agcagcggcg cgtgcgccgt ttacgagcgc ggcggcgatc
     6121 tctccgtgcc ggcccgcgag cgtgaagacg ctgcgcccgt gatcagggtc ggtgtgtacg
     6181 tcgagcaggc gcgccggggt gaacgactgg gccagcgcct cgatcgcctc caggtcgcgc
     6241 ccctcgctga tgttcggcac cgtaagcagc atcgcttggc tctagactgc ttggttcggc
     6301 agggctccgt agctcagcgg ttagagcgca ccactcataa tggtgaggtc ggtggttcga
     6361 atccacccgg agctatctcc aggatcccgc tctgcagcgg gattctgtcg ttttggggga
     6421 gcggccgcac gagctcgcgc gtgcccgatg ggcaggattc aggggtcggt tgcgtctaac
     6481 tgatcgggag gtgtgaacat cgatgaataa tctcgctcag catcggcggc tcctgatcac
     6541 gcttgctgta ttgatcgtga tcgcgctcgc catcacgctg atcgccgtct attccggcgg
     6601 tggtggcggc ggcggcggct actagccggg attgcggctt cgccgcctcg gcgcggccgt
     6661 tcgatcacgc cgatcggaag accagcaaga gaggggtggc gccccgtcgg ccgtcgcccc
     6721 tcaccttgtg gaagcgggtg gcagggttgt gccccacatg gcggccaccc gatactggac
     6781 cggctatgcg gcgccagaac gtggagccgg tgcgaaggcc gctgtcgctc cgagagcgcc
     6841 cgagtcggac ggtggaggag cggctggcac ttcgatttcc gtggttcgtg ggactgtgga
     6901 ctcgcgcgat cgcaagactg tcgccgaggt cccgtctgcg gcaggcactt ctgctccgag
     6961 cgatgcaggt cggcctccag gcctacaacc gtggcgacct agaggtcgtg gagctcgcct
     7021 ttcacgagcg cgtggagttc gagtcacccg gaggagtcgg gaggctcggc tttgaggcga
     7081 gctaccgcgg tcgcgagggg caccgtgcgt tcgaagccga atggcgcagc ggctgggctg
     7141 cgcttcggat tgagccccgc gagttcatcg atctcggcga gcaggtgctc ttgctcgcgg
     7201 agatcgttgg acgcggcaag gggagcggca tctcggtcag ccagaacatc gccacgttgg
     7261 aaacgcttgg cgaggacggg aagatcgttc gagagcagcg gtacttcgac cattccgagg
     7321 ccctcgaggc cgtggggctg ctggagtagg tgtcctgcca ctcccgccgg gcgctcagcc
     7381 ggcctccgtg gcagccgcgg cttcgcccct gcgcgcggcc tccgcgtgct ctttcgagtc
     7441 gcgcgaggag atcagcaccg ctgcgaggac ggccccgagc agcgcgattc ccgtgcccgc
     7501 cagaaacgcg tcctggaagc cttcggtgag cccgacgatc cgcgacttct cgcccgccgc
     7561 gaacacgtcg ttggtacgcg agttggcgac cgaggcgagc acggccagtc cgagcgcgcc
     7621 gcccacctgc tgcgaggtgt tgatcaggcc agaggccagc ccggcctcgt tgggtttggt
     7681 cccagtcacg gcagcgatcg tgtctggcac gaacgagagt ccgagcccga cggccgctag
     7741 cagcgatggc ccgaggacgt cactcgtgta gctgccgcct ggcgcgctca ccttcgagaa
     7801 ccagaagagg ccggcggcca cgaagagcat cccggccacg agcgttggct tgaagccgac
     7861 tcgcgttacg agctgtccgg caatgcccgc cgagatgatg atcgtgaccg caagtggcaa
     7921 gtaggagacg cccgccttga gcgcgctgaa cccgagcacc tgctggaggt agagcgagat
     7981 gaagaagaac atgctgaaca gcgacatgcc gatgagcaat ccgacaacgt tcgcgccccg
     8041 gagggtgcgc agccggaaga tgctgaacgg catgagcggg ttgcgctcgc gcatctcgat
     8101 gacgaggaag atggcgatca ggaacaccgc tccggccagg cgcgcgatcg tggcggtgga
     8161 gccccagccg tcggtcggcg tctgcacgaa gccgtagacg agcagcgcga ggcccgcggt
     8221 gaccgacacg gcgcccggga tgtcgaacga gccgcgcgcc tcctcgggca cgctgctctc
     8281 ctcgaggagg cgcggggcga ggaacgcgac gaacaggccg atcggcgtgt tcacgaagag
     8341 cacccactcc cagccggccc attgcgtgag cacgccgccg agcaggacgc cggccgcacc
     8401 gccggcgccg gcgactgcgc cccagacgcc gagcgcgcga ttccgctcgg cgccttcggc
     8461 gaaggtggtg gtgatgatcg acagggcggc cggcgagacg atcgcggcgc ccaggccctg
     8521 gagcgcgcgg gcggcgatga gccagccctc cgattgcgcg aggccgccga gcagcgaggc
     8581 gatcgagaag aggatcaggc cgtagatgaa catgcgccgg cggccgaggt agtccgcgag
     8641 ccggccgccc agcagcagga agccgccgaa cgtgagcaca taggcgttca cgacccagga
     8701 gaggttgtcc tggctgaagt cgagcgcttt gccgatcgac ggcagcgcga cgttcacgat
     8761 cgaggcgtcg atgacgatca tgaactgcgt catcgccagc aggaggagcg cgaggttctt
     8821 cgcgcgcgga gtatccgttg cggtcatctc gggctttgtc gttcggggga gacgggcctg
     8881 aggcgctgcc cggggtacga gccgcgccgt gttcgaggct caaccttcag ggtacggatc
     8941 ggggatgctc catgccctct cccgcgatca gctgctgccc gcggagccgg gcgaggtgtt
     9001 cgatttcttc gcggacgcgc acaacctgga ggcgatcacg ccgccgtggc tcgggttcca
     9061 ggtgatcacg ccggggccgg tcggcatggg agtgggcacg cggatcgact accggcttcg
     9121 gctccacggc gtgccgatcc actggcggac gcgcatcgag atctgggagc cgggacgccg
     9181 cttcgtggac gcccaggagc gcgggccata cgcgctctgg caccacacgc actccttcga
     9241 aaagcgcggc ggcgagacgc tgatgcgcga cgtcgtgcgc tacgcggtgc cgttcggagc
     9301 gctgggcgaa gtcgcgcgaa ggctgctcgt ggagcgggac ctggagcgga ttttcgacta
     9361 ccgcgccgcg gccgtggccc ggaacttcac aaagtaaagt aagactgagt gggtacgttc
     9421 agacggcttc tgggcttcct gcggccatac cgccgcgggg tgatcgcttc gctgctgctc
     9481 gcggcggccg cgatggtgct cggcgtcctc attcccgcgc tcgtgggcaa gacggtcgac
     9541 gcggtccgca acgggcactc gaacctgtgg ccgtacgccg ccgccatcct cgcggccggc
     9601 gtggggcggc tcgtgctctc ggtgttccgc cgactcgtcg ccggccgcgt gtcgctgggc
     9661 gtggagttcg acctgcgaaa tctgatgtac cggcacctgc aatcgctcga gctcggcttc
     9721 ttcgattcgc agcagaccgg ccagctgatg tcgcgtgcga ccgtcgacct gcagtcggtg
     9781 cgcttcttcc tcggctacgg cctcgtgttc atcgcgcagt cgctgatctc gatcctcgtc
     9841 gccgcggtcg tgatgttcgc ggtcaacccg gggctcgcag cggtcacgct cgcccccacc
     9901 ccgttcgtga tctgggtcgc gacgcagtac gggcgcaaga accgcccggc ctcgcaggag
     9961 gtacagcagc ggatcgccga gctgacggcg gaggcggagg agaacatctc gggcgtgcgc
    10021 gtggtgaagg cgttcgcgca ggaggagcgc cagttcgcgc gtttcagccg gcaggtcggc
    10081 cgcgtgttcg accagtcgat ggtgtcgacg cggctgcgcg cgttctacaa ccccttcatc
    10141 ggcttcctgc cgaacctcgg cctcgcggcg ctgctgttcg tgggcgggcg gcaggcgatc
    10201 aacggaacga tcagcgtcgg cgagttcgtg gcgttctacg gatacgtggc gctgctcact
    10261 cagccgatgc ggatgctcgg cgtggcgctc gggatgtcgc agcgggcggt cgcgtcgggt
    10321 gcgcgcgtgt tcgaggtgct cgaccgcgag ccgcggctcg agtcgaagcc ggacgcgcag
    10381 ccgctgccgg agggcggcgg gcgcgtggag ttccgtgacg tgagcttctc ctatggcggc
    10441 gaggacgcgg agccggtgct cgacgagatc gacctgacgg tcgaggcggg cagcacggtg
    10501 gcgctcgtgg gagcgaccgg ctcgggcaag agcacgctcg tgatgctcct gccgcggctg
    10561 tatgacgtga cctcgggcgc gctgctcgtg gacggcgcgg acgtgcgcga cgtggacctc
    10621 ccggagctgc gcgagcaggt ggcgttcgtg tccgacgatc cgtttctctt ctcggcgagc
    10681 gtgcgcgaga acatcgcata cgcgcgaccg gacgcaagct tcgagcaggt gcaggaggcg
    10741 gccgagcgcg ccggcgcgac cgagttcatc gacgagctgc ccgaaggctt cgacacgctg
    10801 atcggggagc gtggctacac gctgtcgggc ggtcagcggc aacggatcgc gatcgcgcgg
    10861 gcgctgctgc gcagcccgcg gctgctgatc ctcgacgacg cgacctcgag cgtggacgcc
    10921 acgacggaga gccggatcgc gcgggcgctc ggcgatctga tgcgcgagcg gacgacgttc
    10981 atcatcgccc accggctgtc cacgatcgcg ctggcggacg agatcgtggt gctcgaggag
    11041 ggacgcgtgc tggcgcgcgg gacgcacgag gagcttctcg aggagtcgga gctctaccgc
    11101 gagatcgcgg agaaggggct gcccgaccag gtgttcctca cgcgtgatcc gctcgagcgg
    11161 gaggcgacgc tctgatgcgg gcgtgggcgg cacgctggtg ggcgctgttc cggcgcaccg
    11221 agctgcagcg ctcgcgcaag ctgcgcgggc tgctcgagct gctgcgcccg taccgcgggc
    11281 ggaccgtgct gatgttcgtg gcgctgctcg ccgcgaccgc ggcttcgctc gctccgccgt
    11341 acctcgcagg caaggcgatc gacgccggga ttcgcaaggg cgacgtgtcg gcgctcgact
    11401 ggatcgtcgt cgcgttcctg gtgagcgcca tcgtgacctg gacggcgagc tacgtacaga
    11461 cgtacctcgt gaactgggtc ggggagcgcg cgctgcagga cctgcggctg cagctgttcg
    11521 cgcacttgca gcggctgtcg atcgggttct actcgcgcaa caaggcgggg gtcgtgatct
    11581 cgcggatctc gaacgacgtc gaggcgctcg accagctcgt tacggatggg gtcgtgacgt
    11641 tgttctcgtc gtcgctgacg ctgatcggca ccgcggtgat cttgttcgtt ctcgactgga
    11701 agctggcgct gatcacgttc gtcaccttcc cgatcctcgg gatcgggagc atcgtgttcc
    11761 ggctggtgtc ggccgaggcg taccggctca cgcgcgagaa gatcgcgtac gtgacggcgt
    11821 acctgcagga gacgctgtcg gggattcgcg ttgtgcgcgc gttcggccag gaggagcggc
    11881 acctgtcgca gttcgccgat ctgaacgagg agaaccgcgc ggcgaacatg aagacggtca
    11941 atctgaacgc ggcctacttc ccggcggtgg agttcctgtc ggcggtcgcg accgcggtga
    12001 tcctgttgta cggcggcaac caggtgctca acggcgggga cgtgacgatc ggcgtgctcg
    12061 cctcgttcgt cttctacctg aacagcttct tcgatccgat ccagcagctc tcgcagctgt
    12121 acacgaccta ccaggcgggg atggcggcgc tcgacaagat cttcgagctg cttgatgagg
    12181 atccggacct gaccgaccgc ccggacgcgg tcgagctgcc gacggtgcgc ggcgaagtgc
    12241 ggttcgagga cgtgtcgttc tcctacgagg gcgagggcgg cgagtacgcg ctgcgcgaca
    12301 tcgacctcgt cgtgccgcct gggcagaccg tcgcgctcgt tggcgagacg ggcgctggga
    12361 agtcgacgtt cgcgaagctg gtcgcgcgct tctacgaccc gacgtcgggc cggatcctgg
    12421 tggatgggta tgacctgcgc gatgtgaccg agcggtctct gcggtctcag ctcggtgtgg
    12481 tcccgcagga gggcttcctg ttctcgggaa cgatccgcga caacatcgct ttcgggctgc
    12541 gtgacgcgtc ggacgatgag gtgcgggcgg cggcgcgtgc ggtcggggcc gacgagttca
    12601 tcgagcggct gcccgagggt tacgacaccg aggtgggcga gcgcggcggg catctctccg
    12661 ccgggcagcg ccagctcgtg gcgttcgcgc gggcggcggc ggtcgacccg cggatcttga
    12721 ttctcgacga ggcaacgtcg aacgtggacg tgcacacgga ggggcggatc gagcgcggcc
    12781 tgcgccggct gctcgcgggg cgcacgtcgt tcgtgatcgc gcatcgtcta tcgacgatcc
    12841 gaggcgcggg ccggatcctc gtgatggagc acgggcggat cgtggagcag gggacgcacg
    12901 acgagctcat cgagctcgac ggtgcctacg cccggctcta tcaggactgg gcggagcagg
    12961 cggcggcgta agacgaaaag aggcgcccga tgggcgcctc atggctgcag ctgcgggaca
    13021 gggactcgaa ccccaacttc cgcatccaga gtgcggcgtc ctaccattag acgatcccgc
    13081 aatgcctaac ggcagatgat tgtagcggcg gggcagacag cgccaaacct gcctcgacca
    13141 tgccgtccgg acgggaacgt atgttcgtgt aatgcgcgaa cgcgtggcag aactcatcgc
    13201 ctcaggactg agcaggcagg cgatcgcgcg ggagctcggg gtcgcgaaat ccacagtgtc
    13261 gtatcacgcg cgccggctcg gcgaacctgg atcgccgcag ttcggacggc gctatgactg
    13321 ggccgagatc gggcggttct acgacgccgg ccacagcgta cgagagtgcc aggagcgatt
    13381 cggtttctcg aagcaggcct ggaacaacgc cgtgcgacgc ggcgacgtcg tggctcgccc
    13441 gcggctcacg ccgctggcgc acctgctcgc tgtcgacgcg ccacgcagtc gctggaacat
    13501 caagcgccgg cttctcaaag agggtctgaa gcaggcgcgg tgcgagcagt gcgggctcga
    13561 gcgctggcgc ggcgagccgc tgcaactcgc gctccatcat gtcaacggcg atcccgagga
    13621 caaccggttg gagaacctcg ccctgttgtg cccgaactgt cacagccaga ccgagaactt
    13681 cggggtcaag aacgtcgcga gggaggcgct cgtaggctga cggcatgccc gacatcccaa
    13741 ttcgcggcga ctccatccgg ctcgggcagc tcctcaagct ggcaggcctg gtcgagggcg
    13801 gcggcgaggt caaggcgctc ctcgcggagg agcccgtgct cgtgaacggc gagcgtgaga
    13861 gccggcgcgg gcgacagctc caccccggtg acgtggttca gctgggcggc gaggaaatcc
    13921 gcgtcacgag cgcctgatca cggctacaac gccgccaggt ccgtgtcccc gtcggccaac
    13981 gcgcccggct cgacgggcgt cttctccttg atgaagcgcc gtgcgtgctc gagatcctcc
    14041 gagcgaccga cggccatggc ggccttcacg gtctcgtcgt ccagccagaa cgccgtgaag
    14101 tccccctcgt cgagcgagcc gcgcacgacc agatcgcccg agcccggacc gacgtactcc
    14161 atcccggtcc agtcggccag atcagagaag aaatagggca cctcgtcgtg cggcacgtcg
    14221 cggccgagca tgttcagggc agcggtcttg ccctgggaca acgccacgtc ccagtgctcc
    14281 acccgcagcg gcccgccgtg gatgacgctg tcgtattcgc agatgtcgcc ggcggcgtag
    14341 atcccgggcg ccgacgtctc gagccgcgac gagcacttca cgcccccgtt ctcgccgagc
    14401 tcgagtccgg cctgtcgcgc gagcatcacg tcgggcatca ccccgatccc gatcaccacc
    14461 atgtcgcact cgagctcgcg cccggacttc agcacgacct tgtgcacgcg cccgtctgag
    14521 ccctcgaagc ggtcgagctc ctccgacccg tacacctcca ccccgcgctc ctcgagcacc
    14581 cgctggaagt agccgccgac ctccggcccg tagaagcggt cgagtacgac ctcctccatc
    14641 atcacgatcg agcagcgctt gccgaggtcc gtcaacgagg cggcggcctc ggtgccgatg
    14701 tagctgccgc cgatcagcac gacccgctcg gcgtcgttcg catcggcctg gatcgcgtcg
    14761 ctgttcccga acgtgcgcac gtagtggatc ccgtcgaggt cgcagccgtc cacccgcagc
    14821 ctccggacgt tcgcgcccgt cgcgagcagc gccttggcga agcgcacctc gtcgccgctc
    14881 gagagcttcg ccgtccgcga gccggggtcg agcttcatca cgctcgttcg agtgagcagg
    14941 tcgatctgct gctcctgcca ccagtcatcg ggccggaaca gcgcgtcggc acggctctcc
    15001 ttcccgcgga ggtagccctt cgtgagcggc ggccggctgt acggcgggtc gggctcgcgg
    15061 cccacgagga ggatcgaacc gtccgcgcct tcttcccgca gccaccgggc gcagttggcc
    15121 gcggcgaggc ccccgccgac cagcaggtag tcggcctccc tctccgccaa cggcctagga
    15181 gacctctact ggcggccgga accgctccat gatcttctgc tcgaggccgg tgtcgagcac
    15241 gagcagcacc tcgtcacccg cctcgatcac ggagtcggcc agcggcacga acccagtgcc
    15301 gtcgcgcagg atcgagatca cgagggagcc gtccggaagg cccagctcct gcacctgcga
    15361 gccggccgcc ggcgagccct cagccacctc gatctcgatg atctcgagcc gctcctgggg
    15421 cagatcgagc agatgcagga acccgtactt cggcacctcg tgctcgatca gacggaggat
    15481 caggtcggtg gccgacaccg ccggcttcac gccgagaagc tcgaagtgct ggaggttgcg
    15541 cgggttgttg cagcgggaga tgacccgctc caccccgtac ttctcgcgcg ccacctggca
    15601 gatgaggatg ttgtcctcgt cgtcgccggt caccgcgatc accatgtccg cacgtgcgat
    15661 gccggcgcgc tcgagcaccc acagctcgga gccgtcgccg tagtgcacgg cgtgctcgag
    15721 ttcttcctcg acgacctggt agcgggcgcg atcggactcg atcagcgtga cctcgttgcc
    15781 cttgccgatc agctcgcgcg cgaggttcca gccgaccttg ccggctccga cgaccacgat
    15841 gtacatcgct aagcgttctc cctcacggcg agttcgagca tctcgatggc cttctgcgtg
    15901 ggagacacgg ttgaaagccc ctgttccgcg taccacgcgg cgcgagcggg gtcaagaacg
    15961 cggaccacga cgcgcgggat attgaagcgt cgctgggcga tctgcgcgat caccaggttg
    16021 gtgttgtccc cgtccgtgga tgccacaaac gcgtccgccc gctcgatccc cgcctcgatc
    16081 agggcatcga tctcgagggc ggttcccacg gtgaacatgc cgccctggtc ctcccaggta
    16141 tcggtgtagc cgctctcgag cagcgcgagt gcctcggggt cctcgtcgat gacggacacc
    16201 tcgtgtccct cggcgagcat cgcccgggcg attgcggacc ccacccgacc gcagccgacg
    16261 atcagcacga acatctaggc cgcactctag cgagagcggc ggaagcgccg gggctagggc
    16321 gcgacgccca ttctcgtgcc cgcctccccc tcgggagcgg cggtgaggat tacgcggcag
    16381 ggggcctttt cgaacacgta gcgggtcacg tcgcccacca ccttgtcgcg cgggcccccg
    16441 cgcccgccca gcagggcgcc gccgcggacc ttcgacggcg actcggcggc aagcacgatc
    16501 acgtccacgc cgcgccgtcg cgcctcgtcc acgatcccgg cgcccaccga gcgcgcgcgc
    16561 accaccgcgg tcgccacctc gacgccctcg tactcctccc ccacctcctt ggcgcgcgag
    16621 agcgccttcc gcgccgcctt gatgcgatcc tccggcatgc gcgcgtcgag cggcagcgac
    16681 atcggcacct cgagcacgta gagcgcctcg atcacggtgc cgccctcacc ggggtcgccg
    16741 tcctccgacg cgagccgccc ggccgtgccg atgatgtcgt cgtccaggtc ggacccgaag
    16801 acgggcacga ggatgctccc gtactcaacc tctggcgcgt cctgcagcgc ggtggggggg
    16861 atcgtgaagc ggcgcgtgag cgacttgccc gccgtcttcc ggtagatcac gtagagaagc
    16921 agcccgcctg ccagccagcc gaagccgacg tagcgggcgc cggaatgcag gatcacgacg
    16981 ctcacccagc cggcgccggc gaggatcgcg ccgagcaccg cgggcaccgg caggtccgcc
    17041 ccgcggaccc tcacggacca gggaatgcgg tagggacgcg gcaggtcggg ctcgcggaag
    17101 cgcagcgtga tgatggacaa gtgcgcgagc aggaacgcga gcatcgcgcc gaaggcgaag
    17161 atgcccgcca tgaacttcag gtcgtggctg aacacgagcc cgaacgccat cagcgacgcg
    17221 acgatgatcg ccacgtacgg cgtggagtag cgggccgtga ggcggccgag gtcgctcggg
    17281 atctgtctat tggtggcgag cgagtaggcg agccgggcga acccgagcat ctgcccgttc
    17341 acggcggtca tgagcacgag cgcaccgacc gcgccgagcg agtagcgcag cgcgtgcatc
    17401 agccagtccg ggtcgtagga gttggcgacg ccgagcagcg gctcctccac gtagcgggtt
    17461 ccgagctgcg tctcgccgtt caccacgggc acgttgagga gcgccacgat cgagatcatc
    17521 acgagcacga tcagcgccga gatgccgcct acgaagacga ggcggcgaag cgcccgccgg
    17581 cccacgcgta tctcgctggc gagtcccgac gcggcctcga tcccggtgag cgcgaccgtc
    17641 gccaccgccg aggcgaagat caggtcctcc caggtcgggg agctgcccag gtggatgctg
    17701 tcggtgatca ggccgaaatc ggacgaattg gcgaagcccg cgatcacgac tacggccagc
    17761 agcacgaggt tcgcgatgcc gagccgcagc aggaattgga gactcatcgg cgagagcccg
    17821 cggatgttcc accaggccac ccacgcgatc acccccgcgg cgatcaccag ctccaggccc
    17881 gagctgtcga gttgtcccca gaacccggcg aggtagtgcg agaccgtgaa cgacgccatc
    17941 gccatcacga tcaggtagtc gaggatgatc gcccagccgg cgatgaagct ccagagctcg
    18001 ttgaaggcgt agcgcgcgaa cgtggacgcg ccaccgcgct ccggatggag ggcgttgccc
    18061 tccacgtagg tcatcatcgt gagcacgaag aagaagctcg cgccgaggaa cacgagcggc
    18121 gtcatgccga gcgcgtcgcc cgccaccacg ccgagcacga agtaggcggc cgagccgacg
    18181 ctcgtgagcg ctatcgcgaa gagcgccggc gagccgacgc cgcgccacct cacgtcggag
    18241 gccgcgagcg cggctcctgg cgggtggcga ggtagaagcg cgctgcgccg agcagcgcga
    18301 acaggacgcc caggaccacg ccgagcgcca gcggaccacc tccgcgcgcg agcgtgacta
    18361 cgacgatgac gaccccgagg acgaagatca gtgcgcccat cacccgtgtg gactctctgt
    18421 atgcgcgctg cacgcgcggg atgttagacc cgccttctgg actagtggaa accgggttcg
    18481 gcggccgctc tcaggcggcc aggcgacgct cgtgcgtgcc gttccccgac tcgatgatga
    18541 cgcgcgcggg ccgctccgcc agcaccgtct cgatggtgcg gccgaacggc gtgccgccgg
    18601 cgcgcctccg cggcaccgac atgacgatcg cccgtgcgcg gatgtcacgc gcctcgtcga
    18661 cgatccgccg gccggcctcg cccggacgga ccttctccca gcggccggtc acgcgcctgc
    18721 ccccgagcac atgcgcggag tcaatggtgg cctgtgccac ctcctcctgc tcgggcagct
    18781 ccgcgtcgat cggcgagctg ttcggcaccg tgatcagcac gagcacgtag atcccgcggc
    18841 ggcggcgcgc ggcgagcttg gcggcggtcg cgactgcctc gggctcgtac tggtcgtcct
    18901 cgaaggcgac gaggatcgac tcgtactcca cctcgtgctc cacgatcggc tggggcagca
    18961 cgatcttcgt cgtctgcgtg aggctcaggc cctggcggcg tcggtagatc acgtaggtcg
    19021 agatcccgat cgtcagccaa accgcgcccg ctatggcggt ggacgcgttc agcacggtca
    19081 ccaccaccca cgccgacgcc gtgccaagtc cgcccgctac ggcgaagaac ggcaggttcc
    19141 ggcccgcgac gcggatctgc cccgggcctc gccaggggcg ctcgacgtcc ggctgattga
    19201 tgcgaagcgc gatcaccgcc gcgtgcgcga tcgtgaacga gagcatcgcc ccgaaggcgt
    19261 agatctggcc gaggaagtcc gccttgcccg ggatgatcgt gaggcacgcg accgcgccga
    19321 acatgatgat cgcgatgtac ggcgtgcgga agcgcgggtg cagctgacgg agtcgctcag
    19381 gcagctgccg gtgctgcccc atcgagtagg tgagccgcga caccccgatc agcccggcgt
    19441 tggtggcgat gaacaggatc gtggcggcca ggatgcccac gtagatctcc gcggggtgct
    19501 gcaggaagcc gaggtccatg ttcttcacca cgccgagaat gggatcgccc gcgtagccgc
    19561 ccttctcctc ggacagcgcc agaagcgtct ggccgttctt gaccggcatg gcggaaagcg
    19621 cgatcgacgg caggaacgca tagatcgcga ccacggccgc gaccacgaac ccgatcgatc
    19681 tcgggatcgt atgcccgtag tccctcgcct cctcggccat gttcgagatc gtctcgatgc
    19741 ccgtgtaggc gaccatgccc accgggatcg cgatcaggaa gtcggtgacc gtcgggtagg
    19801 tgccgaagtc gatgttgttg acgagcgtgg acggcgagaa caccagcacg aagcccacga
    19861 tcacgagcag cagctgggtg atgaaatcgg cgatcgcgag aaagacgttc agcccggccg
    19921 cttccctcac gcccacgatg ttcaggaggc cgagcagcac caccacgccg atgccgaaga
    19981 agatgtcgcc gggcgaggag cgcagcggct cccagaacac gccgaggtag tgcggcacga
    20041 agaacgccga gatggcgacc gtgatgatgt agttgagcat ctggccccag gccgcgaaga
    20101 aggaccagaa ctcgttgaag gcgtgccgcg cgaatgacga ggagccgccc gcctccgggt
    20161 acatcgcggt cgcttcggcg taggtggcag ccgtggccgc gaagatcagc ccggagatga
    20221 tgaacacgac cggcgtcatc ccgagcgcga acgacgccac gaggcccagg gcgtagtaga
    20281 tcgagctgcc cacgttgccg taggcggtgc tgaatagcgc gcccgttccg agcacgcgct
    20341 cgagtccttg gtttttgggc ctgcgttcgg ccatctactc gaaacctaca accgactcgg
    20401 gcgacatttc ccccaaagga ccagaaaaag aaccgggccc ggggagaaat cgccccgggc
    20461 ccggaaaggc ggcgaaaacc aagctgggca aggacgcagg gagcgacgtg tgctctgcac
    20521 acgagtctcc cgaggacgcc gcccagcgcc ggtttgcagc cgcctctaca cgaggaacgg
    20581 gatgatgagg atggcgacga tgttcgcgac cttgatcatg gggttgatgg ccgggccggc
    20641 ggtgtccttg tagggatcgc cgaccgtgtc gccggtgacc gacgcggcat gggcctccga
    20701 gcccttgccg ccgaactcgc cgtcctcgat cagcttcttg gcgttgtccc acgccccgcc
    20761 gccggaggtc atcgagaccg cggcgaagaa gccgaccacg atcacgccga tcagcagccc
    20821 gccgagcgcc tgatacgaga gcaggcccac gatcagcggg atgaggatgg ggatgagcgc
    20881 cggaacgatc atctccctct gggcagcggc cgtcacgatc gacaccgcgc gggcgtagtc
    20941 cggcgtgtcc gtgccctcca tgatcccggg cttctcgcgg aactgccggc ggacctcctc
    21001 gaccacgccg ccgccggcgc ggccgaccgc ctcgatcgcg agcgccgaga acaggtagac
    21061 catcatgccg ccgagcagta ggccgatcag cacctccggc tgggagatgt cgaacgactt
    21121 gagcggcttg ctgccggcct cgtcttgcag ctcgatgatg aaggcggcga acagcacgag
    21181 cgccgcgagc acggctgagc cgatcgcgta acccttcgtg accgccttcg tggtgttgcc
    21241 gacggcgtcg agcggatcgg tgacgttgcg tacctcctcg gggaggtccg ccatctcggc
    21301 gatgccgccg gcgttgtccg tgatcgggcc gaacgcgtcg agcgcgacga tcaggccggt
    21361 cagcgacagc tgcgccatca ccgccacgcc gacgccgtag atgccggcca gctcgttggc
    21421 gcccaggatg ccgagcgcga tcaggatcgc cggggctgcc gtggactgga agccctgcgc
    21481 gaggccctgg atgatgttcg tggcatgacc cgtctgcgac gcgcgcgcgg tggtgcgcac
    21541 cgggcggaag cgggtggacg tgtagtagtc cgtgatcacg aacaggccgg cggtgacggc
    21601 gaggccgatc agggtgcaga gccagtagtc ccaggggcct gcgccgatcg cgccgtggag
    21661 caggctggga gcgtcgggcc gcacgcgcag gtcgtccatc agccactgcg tgacggggat
    21721 gaaggcgacc gcggcgatca ccgctgacgc gatcagaccc tggtagagcg cgcgctcaac
    21781 gttcccggcg cggctacgga cgaagaaggt gccgacgatc gacgcgagga gtgacacgcc
    21841 gccgacgacg agcgggaaca ccgcggcgtc gcgctggttc gtgaacgtga gaacgccgag
    21901 aagcatcacg gcgaccgcgg tgaccgcgta ggtctcgaag aggtcggcgg ccatgccggc
    21961 gcagtcgccc acgttgtcgc ccacgttgtc ggcgatcacg gccgggtttc gcgggtcgtc
    22021 ctcgggaatg ccggcctcga tcttgccgac caggtccgcg ccgacgtcgg cggccttcgt
    22081 gaagatgccg ccgccgaggc gggcgaacac cgagatcagg gagccgccga agccgaggcc
    22141 caccagggcg tcgacggcgg tcttgtcgct ctcgccggcg aggagcagta cgccgtagta
    22201 gccggccacg cccaggagcg cgaggcccgc gaccaggagg ccggtgaccg cgccgccgcg
    22261 gaacgcgacc tcgagcgcgg cgctgacacc gctgcgggcg cgctccgcca ctcgtgcgtt
    22321 cgcgcgcacg gagacgttca tgccgatgaa gccggccgcg cccgagagcg tgccgccgat
    22381 cacgaatccg atcgccgtgc gaccgtcgat cgcgacggcg agaacgatcg cgatcacgac
    22441 ggctacgccg gcgatgatct ggtactggcg cttcaggtag gcctcggccc cctgctgcac
    22501 ggcgcgagag atgttcgcca tcgtctcggt gccgggcgac atggccagca gccagcggga
    22561 cgtggtcagt ccgtagacca ccgccgcccc ggcgcagcca agcgcgccga tgattcccca
    22621 atcaactgtg ctcaagaagt agtcctccgg attcggatgc cctgggcccc gcgtgcgggc
    22681 gccggatgtc gcgtgcgcag tcgcttcagt gtgctatcgc gctttgaagc gccgtcgcgc
    22741 agatgtcttc cggctgcggc ccgattctgg gcaccgcctg gaacaagagg tcgcgcagtt
    22801 tagcgaggtt ctcggcgaaa acccggagcg ccatctcggc cgagaccggc tctacgtgcg
    22861 ggcttccctc gagtccgacg tcgtagtccg taacgagcga cacgtttgcg tagcagagct
    22921 cgagctcgcg cgccagccag gcctcggggt actgagtcat gttcacgacg tcccacccgg
    22981 ccgccgcgaa ccagcgcgac tcggcacgcg tcgagaagcg cggcccctgg atcacgacga
    23041 ccgtgccgcc atccaccacg gggatgtcga gccggcgtgc gcagtcgacc aggatgccgc
    23101 gaagctccgg gcagtacggc tccgcggcgg acgcgtgcgt ggtgcccggc ccgtcgtaga
    23161 aagtgtcgcg gcgcccgctc gtgcggtcga cgaactggtc gactacgacg aacgtgccgg
    23221 gctggagctc gggcttcagc gagccgcacg cggacggccc gatcagccgc cgcactccca
    23281 cctccttcat cgcccagagg ttcgcgcggt agtcgatgcg gtgcggcggc agctcgtgct
    23341 tgtcgccgtg ccgaggcatg aacgccaccg ccgtcccgtc gatgtgaccg atccggatcg
    23401 gggcggagtg aggcccgtac ggcgtctcga ccgtgacggc ctcggtgtcg tcaaggaact
    23461 cgtagaagcc ggagccgccg aagatgccga cgtgcggccg cgcctcgggg ctcacgtgcc
    23521 gggcggagtc cagatcttgg aacgggcctt ggcgcgctcc tggtcctcag cggacggctc
    23581 ggatggctgg tcctccgctg gctgctcctg tccggcgccg ccgcccgcct cgcgcgcgta
    23641 ggcgacctgc agctgcgaga gcgcgtcgcg cacgggcgcc atctcatcgt cgggcagcag
    23701 tggcagcagc gcacgcaccg cgtcgatgcc gagccgtgcc tgctcgaggt ccttctcctc
    23761 gggcgcgccg gtcagtccga gacggcggcc gctcaggttc acaaggctca ccaccgtctg
    23821 gagcactacg tcgcggacgg tgatcttgcg catctgctcc tcgagcgctg cgcgaagctc
    23881 ctcctcggtc ggctgccgct ggcctccctc ctgtccttcc tgcgtcaagt cgtcaccccc
    23941 tctgccgctg cgtaggtcct ctgcgtagcc tccacctcgg cggcgaagac ctcctccatg
    24001 ctcgcaccac tgcgcagcgc acgtcgctgt cgctgggcgc cgttctcggc cggcacctgc
    24061 acgtcgattc cgagcagcgc ccgcgcgggc gcggtccacg cgagcagccg gtcgggcaac
    24121 gccgcggccg ggaactcctc gaggcggtcg aggtcgagca tcttgccatc gagcccgaag
    24181 cggatcgcgc gccagaagtt ctcttcgatc agacgccgcg gtttcggctc gtacggcacg
    24241 ctctcgtcgt ggtccatcac ggcctgcgcc aagcaggccg cgatgagcgc ctcgagcgcg
    24301 ctcgactcct cagccgtgct ctgcgcgtcg cagatgcgca cctccacggt gccgaaggag
    24361 cggtggggcc ggatgctcca ccagatctgc gtgtcctcga cgatcgagcc ggtccggtag
    24421 agcatgtcga tgtactcctc gtacacggcg gcagtgccgt agtgatccgg aatgccgcag
    24481 cgggggaagc tcttggtgaa gatctgggtg cgaacggtgt gcaggccggt gtcgcggccc
    24541 tcgaggaaga tcgagttcgc cgagatcgcc agcagctcgg gcagcacggg gcgcaggcgg
    24601 tcgcacaccg cgactgcgcg gtcggcgtcc ctcacgccca cgtgcacgtg ggtgctgaac
    24661 gtgttgttgc gccacgcgac gtacttgagg ccctcctcga cgcgccggta gtgctcggtg
    24721 tcgatgatgt gctgctcgcg gtaatcggcc cacgggtgcg tcccggtcgc acacagcagc
    24781 acaccgtggt cgccggccag acggaacagc cgccggcgcg cctcctgctg agcggccagc
    24841 gcgtcggcga gcgtctgccc tcggcccgag cggatctcga tctccgagga gatcagctca
    24901 ccggcgatcg agtcggcgag cacgtcgtcc tgctgcgcgg cctggcggag ctcctcgaag
    24961 cgctgcgcga gatcccggct gcccgggtcg aggatcccga actcctcctc gagcccaacc
    25021 gtgaagtcct cggaggcctc gaagacctcc cgcagagcag ctgcgtgcat cgcggtgggc
    25081 ggggtctacg tcaaatagaa gtagagcgcg tagagcagat cgacggccgg gctcgacgtg
    25141 tgcaccgtca cctccggcgg gacctgcagc agcgcctcgg agtagttgaa gatgatcttc
    25201 acgccggcct cgacgaggtc gtcggcaacc gcctgggcgg cctccgtggg caccgcgatc
    25261 acgccgacca cgatgtcctc ctcctggacc accttgtcga ggtcgtcggt gggccgcacc
    25321 agcagtgcgg tcttgccttc gccgacctgc tggccgatct tgcgctccga gttgtcgaag
    25381 atggccacga cgcggaaccc gtggtccgcg aagatgtccg agctcgcgat cgcctggccg
    25441 agatggcccg ccccgaacag cgcgatgttg tgctgcccgg ccgtgcgcag gatctttcgg
    25501 atctgcgaga cgagcgagtc gacgttgtag ccgacgccgc gcttgccgaa cttgccgaag
    25561 ccggataggt cccggcggat ctgagttgag ttgacgtgtg tgtagtccgc aagctcctgg
    25621 gaggagatcg tctccttgcc catcttcttg gcctgcgtga gcacctggag gtagcgagag
    25681 aggcgggcgg caacgcctag cgataggcgg tcgccggtgc cctcggccac gtccgtgctg
    25741 ccgtcataga gctggttgat ggtcacgtct ccactctact gacagagtcc gcgagcgcgt
    25801 ccgggtcgac gtggtactcg aacagctcct cgccatcgac tgccacgacg gggatgcgtt
    25861 caccgtactg gcgatgaaga gccgagtcga ggctcacgtc cacctcctcc agagcaaagg
    25921 gcgtgctttg gcgcacgctc agcaccacct cacgcgcatc gtcgcagagg cagcagccgg
    25981 gcttgccgta gagcgtgacc aatctcacgc ggccaccggc ctcgcggacg cgtcgagcgc
    26041 cagatacccc gggaaaggaa gcggctcctg gaagcgagcg gcgcccgcga tcatcgccgc
    26101 gttgtcggta cagagcgaaa agtccgggat ccataccttc aggccgagct gggccacgcg
    26161 ctcgcgcaac tcccggttcg cggccacgcc gccgccgatc gcgaggcgct cgagcccctc
    26221 gcgctcgatc gccttcttcg cgcgcgcgac gagcgcatcc acgatcgcgc gctggtagga
    26281 ggcggccagg tcagccttgc gccgctccgt ctccgtctcg ccgaggtcgc gcacggcgta
    26341 cagcagcgac gtcttcagcc ccgagaagct gaagtccagc tcccccggcg ccgagcgcgg
    26401 gaagtcgaac gcggtggggt cgccctcgcg cgcgagccgg tcgatcgccg gaccgcccgg
    26461 atagccgagg ccgaggagcc gtgccccctt gtcgaaggct tcgcccgcgg cgtcgtcgag
    26521 cgtggtgccc agccggcggt gggagctcag ctcgtccacg cgcgcgatga acgtgtggcc
    26581 gccactcgcc acgaggcaga ggtacggcgg ctcgacgggg tcgtcgccca gcgtgctcgc
    26641 gaccacgtgg ccgtggagat gatccaccgg agcgagcggc agccggcgcg ccgcggcgat
    26701 cgccttcgcc tcggagacgc ccacgagcag cgcaccgatg agcccgggcc cctgggtgac
    26761 cgcaatggtg tccgcgccgt cgagcgtggc acccgcgcgc tcgagagcgt cgtcgatcac
    26821 cgcgtcgatc agctcgaggt gccgccgcga cgcgatctcc gggaccacgc cgccgtagcg
    26881 ctcatggagc agtccctgcg acgcgaccac gttcgaacgg acctcccccg cgtgtgagac
    26941 gaccgcggcg caggtgtcgt cgcagctggt ctcgatcccg aggatcacgc cggcgcggcg
    27001 cgctccgagg cctcgcgcgc cgggtcggtg acgggaccgc tcgcatccgc ggtgcgccac
    27061 atgatgagcg cgtcttcctt gttgtcgtgg tagtagccgc gccgcttgcc ggccgcccgg
    27121 aatccgaagc gctcgtacag ggcgatcgcc gaggtgttcg actcacgcac ctcgagcgtg
    27181 tactgctcac ctgggcgatc ggcagtctcg aacaggcggt tcagcagcgc cgtggcgatc
    27241 ccggtgcggc ggcggcggtc gtcgaccgcc acgttcatca agtgccagac cgtgtcgtag
    27301 cgggagcaga cgaggtagcc ggcgatcttc ccgtcgatgg tggcggcgag gcagatcccg
    27361 gacggcttcg acagctcgag tacgaacatg gcgagcgacc aggctgtcgg gaacgcccgt
    27421 cgctcgatgg cgatgacctg cgggagatcc gagtagctga gacggcgtat ctcgagtggc
    27481 ggggtcaact tgggggcttg gcgtccggtt cgcgcagata ctgggggagc accgcatcgg
    27541 caggcaccgc aaaagtctgc cctgccagcc ggcacaggtg aagggccctt acgacgtgcg
    27601 acgtggaatc gggctcggcg acagccacgc cgcccgcctc cagagcctct cgaaatcgta
    27661 gcgagccgtc tccggctgcc agaagcgatt cgtcactcaa caggccgtcg gatcgcaagc
    27721 gctcgatgag cgcctcgggc gtggccacga acggcgcgat ccgctccctc ccgtcgtcgt
    27781 agagcgccgc gaacagttcg ccgcgcttgg cgtcgatcag ggcgaggcga cgcggcgcct
    27841 cgatgccctc ggcgagggct gcgagcgagg acacgggccg cacctcgagg ccgtgtgcgt
    27901 gcgcgagccc gcgggcggtg gcgaccccga ttcggagacc cgtgaacgtg cccggcccca
    27961 cgccgaccgc cacagcgtcg agctgttcga acgacagccc cgcgcgttca aggcagccgg
    28021 tcacctcggg cagcagctcg cgcgcgtgtg ccggcggctc gcctagcgcc tcgaccggtg
    28081 ggacgaattc gaagccggcg ccgtcgtcgc ggagcacgca cgcgcttgtg gccgcggtgg
    28141 aggtatcgaa gccgagcagg ttcactcgtc cggcagctcc agcgcgcggc ggtcgccgcc
    28201 gaggtgctcg atgcgaacgc gagcgcgcac gctgtcgagc ctcgggagcg cgacgtcggg
    28261 ccactcgagg aaggcgatgc gctcgggcgt gacgtagtcg tcgagcaaag ctgggtcctc
    28321 gccctcgagc gtgctcagcc ggtaaaggtc caggtgggcc acctccgggc ggccgtcgta
    28381 cagctgcccg atcgtgaacg tcgggctggt gacgggctcg cgcacgccga gtgcgcgggc
    28441 ggcgccccgc acgaaggtgg tcttcccggt cccgagctcg cccgacacga gcaccacgtc
    28501 acccggcttc aggcgctcgg cgagccgcga tccggcgcgc tcggtctcct cggggctcgc
    28561 ggtctcgatc cgcatggccc gcgaagtgtg gcgggtgcga tcagccgaag agcccgagct
    28621 gcggcggctc ggcggccatc atcgccggct ccggcggccc cgctgcgggc ggcgcaggcg
    28681 gcgcggcagc cttcggccgg gcggtggcga tcagctccgg cagcttctga aagtcgtcgc
    28741 ggagctgggt gagcacggca ccgctgcgga ggcccgcggc cggatggaag agcgggaaga
    28801 tcaccagcgg cagtccctcg atcacgcgtt cctgcgggcg cccgtgcgcg cgtgtgatgc
    28861 cctccggccg gcccgtgatg cacttggtag cgaagttgcc caaggtgcag atcacgcggg
    28921 gctcgatcag ccggatcttc tgcatcaggt agggcttgca ctcctcgatc tcttcgggct
    28981 ccggatcgcg attgccggga gggcggcaca tgagcacgtt ggcgatgaag gcgtcctgct
    29041 cacgctcgag tccgatctca gccagcagct tgtcgagcag cttccccgcc gcacccacga
    29101 acggcttgcc ctgctgatcc tcgtggaagc cgggcgcctc gcctacgaac atcaggtcgg
    29161 cgtcggcgtt gcccgacccg aacaccacgt tcgtgcgcgt cgcggcaagc gggcagtcgt
    29221 ggcagtcctg gagctcgcgg tagagcttga tcagctcagc cctgcgttcc tcggccgcgc
    29281 gtcccattcg gccctcaggc tacggagaac tgcggatgga gttccgcctc gctgggtatc
    29341 cttaagcgca agccgctacc ccatctgggg agcggactga gacacacctc acactcaacc
    29401 gacctgccgc ccggtatctc ggaagacggg aactggagca cgatgagcgc ccaagccacc
    29461 cacgcgcagc ttgcccagca cgggttgcgc agcattgccg tggtcggccg cggccgcctc
    29521 ggcaccgcga ttgcgccggc gctccgcaac gcgggcttcg acgtgatcgg cccgcttgga
    29581 cggggtgagc gcgccgcagc cgacgccgtg ctgctctgcg tgccggactc gcaaatcgcg
    29641 gccgcggcgg ccgcgatcac cggctgcgcc ccgctcgtcg gccacacgag cggcgcaact
    29701 cctctctccg cgctcgagcc ggcccgcggc gagcgctttg gcctccatcc cctccagacc
    29761 ttcaccggcg gcgacggccc cgagagcttc acgggcgtcg gctgcgccgt gggcggcagc
    29821 tccgagcgcg cacttgacgc cgcgatggag atcgccggcg cgctcggcat gcgtcccttc
    29881 gaactcgccg acgagcagcg tgcggcctac cacgccgcgg cctcgatcgc ctcgaacttc
    29941 ctcgtcgcgc tcgagtcggc ggccgagacc gtcgccggaa gcgcgggcct tccgccggag
    30001 cgcacccgtg agctgctcgc tccgctggtg cgcgcaaccg tcgagaactg ggcggacctc
    30061 ggtcccgagc gggctctcac cggacccgtc gcccgcggtg acgagctcac cgtcgccaag
    30121 cagcgcaccg cgctccgcga gcacaccccc gagctgcttc cgctcttcga cgcgctcgtc
    30181 gcgcaggcac gcgccgtcgc cggccgcgga gaggccgcgg catgaaaacc gttcgcacaa
    30241 tcgccgagct gcgcgaggcg gtgcgcgccg ccgagcgccc ggtcggcctc gtcccgacga
    30301 tgggcgccct gcacgagggc cacctgtccc tcatccgccg ggcgcgcgag cagagcgcca
    30361 cggtggtcgt ttcggtgttc gtcaacccaa gccagttccg gccgggcgag gacttcgagg
    30421 catacccgcg caacgagcag cgcgacgccg aggtcgccga gcgcgagggc gcgggtatcg
    30481 tgttcgcccc ttcggtcgag gaggtctacc cgccgggatt cgacacgagc gtgcaggtgg
    30541 gcgcgctcac ggaggtgctc gacggccacc cggcgcagcg tggcccggag cacttccatg
    30601 gggtgacgac ggtcgtcacc aagctgttca acatggtgca gcccgacgcc gcctacttcg
    30661 ggcagaagga cgcccagcag gcgatcgtgc tgcgcaagct cgcacgcgac ctggacatgc
    30721 cggtgcgcat cgaggtctgt cccacggtgc gcgagcccga cggcttggcg ctcagctcgc
    30781 gcaacgtcta cctcgggccc gaggagcgcg agcgcgcgct cgccctttcg cgcgcgctcc
    30841 gcgcggccga acaggccgtg gccgcgggca cggtcgaggc cgacgtcgtg ctcgccgcgg
    30901 cgcgcaccga gctcgaggcg gcgggcatcg agcccgacta cctcgagctc cgctccacca
    30961 acgatctttc cccggtcgag cgcgtgaacg gctcgactct gctcgccgtg gccgcccgcg
    31021 tgggccgcgc acggctgatc gacaacaccg tgctgggagg cgcatgaaac ggacgatgct
    31081 caagtcgaag atccaccgcg ggcggatcac ggcgagcgat cttcactacg tcgggtcgat
    31141 cacgatcgac gccgaacttc tcgaggccgc cgacatcctc gagcacgagc tcgtgcacgt
    31201 gctcgacgtg gacaacggcg cccgcttcga gacctacacg atcgccggag cccgcggctc
    31261 cggcgagatc aaggtgaacg gcgcggcggc gcgactcgtc cacacgggcg acaccgtgat
    31321 cgtcgtgtcc tacgcctcat acgacgagcg agagctcgag tcctacgagc cgaaggtggt
    31381 tcacgtgaac gcgcacaacg agatcattca ggtcgacgac gcggtggcgg agctggtggc
    31441 atgagcgcgc gaccgaacgt ctcccccgcg ccgcccgcaa aacgcaaggc gacgacgctc
    31501 tccacgctcg ccgagaagaa ggcgctcggc gaaccgatcg tcatggtgac cgcctacgac
    31561 tacccgagcg ctcaggtggc cgaggaggcc gccgtggacc tcgtgctcgt gggcgattcc
    31621 ggcgcgatga ccgtgctcgg ctacccgagc accgtgcccg tgtcgatcga cgagatgctg
    31681 atgctctgtg ccgcagcccg gcgcgggctc tccaccccga tgctgatcgg cgacctgccg
    31741 ttcggctcct acgaggtctc gaacgagcag gcgatcgcga ccgcccagcg cttcgtgaag
    31801 gaggccggat gcgacgccgt caagctagag cgcggcggcg tgtcggccga gcgtgcgaag
    31861 gcgatcgtgg acgccgggat cccggtgatg ggtcacgtcg ggctcacacc gcagaccgcc
    31921 accgcgctcg gcggctaccg ctcgcagggg cgcaccgcgg aacgggcgct cgaagtgctc
    31981 gacgacgctc tggccctgca ggaggcgggc tgcttcgcga tcgtcttcga agccgtaccg
    32041 agcgagttca ccgagctcgt catgccgcgg ctggcgatcc ccgttatcgg aatcggcgcc
    32101 gggccgtcca ccgacggcca ggtgctcgtg ttccacgatc tgctcggcat ctacgacggt
    32161 cacgccgccc gcttcgtgaa gcgctacggc aacgtgcgcc aggcgatgat cgacggcgtt
    32221 tcggcgttcg cggcggaggt gcgcgagcgc cgctacccag agcccgacca cggctacacg
    32281 atggcgcccg acgaggtggc gcgcctcaag gacgcgctca cccacagcga gcgcacaaag
    32341 gcctgaacgc ggaggcggcg cccctcgggg cgccgcctct gcctaccagg ggcgactgga
    32401 gcgtctaata gccgcggctc gagccggttg tgtcggtcga gccgttgtcc gtggtggagg
    32461 agccggcgct gccgctgctg cccacggtga tcgtgccctt catgcccatc tgcttgtgat
    32521 tgtccaccgg gcagtaccac tcgtacttgc cgggcttcgc gagatcggcc ttcagctcgc
    32581 tcgagtcgcc tggattcagc tgactcggca gccgcacctc tccgttcggg ccctcgacct
    32641 cgaggctgtg gaccgtctgc ccagagtttt tgaccttgaa ctcgaccacg cccggcttca
    32701 ccgacggatt cgacggattg agcttgaagt cggtctcggt cacgttcacc gtcgccgacg
    32761 cctttcccgt cggggcggct tgcgtggacg tgcccgaggg cgtcgtgccc gtacccgagg
    32821 agcgcttctc gccgcctcca cagcctgcga ccaacagcac ccctgccacc ccggccgcca
    32881 gcaggccggc ataccgcttc gtctgcatcc tccccaggct ccttcctcgt tggtctgttc
    32941 tggaagacgg ggcaagcgtc cagattcatc ccgccgccgg caaatcctca ggcttggcga
    33001 actcgatttc cttgatcacc ttcgccggcg agccggccgc caccacaccg tccgggagat
    33061 cggaggtgac cactgagttc gcgccgatta cgcagcggtc gcccaccgtc acgccgctcg
    33121 tgacgacgca gttcacgccg aaccagcagt tcgagccgat ccgcaccgga cccttcgagg
    33181 tgaagccctg ccaggtgatc ggccgcgtcg ggtcgtcgaa gcggtgggcc gagtcgccga
    33241 cgaagcagtg gttcgcgaac atcgtatggt cgccgatctc gatcgagtcg atcgcggcga
    33301 gcatcgtccc gatgttgagg aagcagccct cgccgatgcg cacgcgtgcc ccctcgccga
    33361 tcgtgatcca gcatcccggc tcgagcagcg tgttggcgcc gatctcgagg cggccgtcgt
    33421 cgaggcactc cagcacgttg ccctgcaccg gaaagcggat gaacgcctcg cgctgcagcg
    33481 catgccagtg gatgcgcgcg cggttccacg gcagcgcgtt tcgctcgtac cagcgccact
    33541 tgcgctccca cagcacggcc ttgcggtaga ggtctcccat cggcgagagc tttccagacc
    33601 gcgcggcggg cccggtgcaa caaaggagcg ggcccccgcc tgccacgggg gcccgcgttc
    33661 gctcgccgga aaccggacgt tccatatccg ctaccggcag tttggcccgt accccatcag
    33721 tcgccggctg cgcgccggcg tagagcttgt atccgtcagc ggcgatcccc ccgttcgcgt
    33781 gccgcctgac gccgtcgctc gggcggcggc cggaagcggt tcgatcgctg cctcgactcg
    33841 gcaggctgcg gactcgggcg aatcggcgtt gcggacgccg gctcgccgtc ttgatccccc
    33901 tcgccgccct cctcctccgg ccagtgctcg aagacgggtc cccagcgtgc gccgagaccc
    33961 gtcatcacgt acgcgtggcg gacgtagtcg tagcggaagc cgagccgcat gagcctggcg
    34021 atcgcccgat cgcccacacg gcgccgtttg cggtgcgtgc ggcgcggacg acgcgggatc
    34081 gaacgtcgcc ggcgcgtccg cgtgccgagc cacaccagtg cgactgccgt ggcgatgatc
    34141 gcgagtatga acagcagtgc gaaggtgttc tcgttgccac caccggcgtg atccgcactc
    34201 ggctcgaccg gcgccgtctt gggaggtggt ggtgccttcg cgggcttcgg ccgctcattg
    34261 ctgctgccgg aggtcggctt cgcgcgctcg tgaccgccac gtgacgccgg cttcgcgcgc
    34321 tcgtggccac cacccgacgc gggcttcgcg cgctcccgcg tgctgcgtgt gcgagcccgc
    34381 tgcgccgtcg gcagctcagc ctgcgtgaga ggaccggcaa ctccatccgc agcgaggtga
    34441 cgcgcctgct ggaacctgac cacggcgcgc gtggtctgcg gtccgtagag gccgtcgacc
    34501 ggacccggct ggaagccgcg gtgtgcgagc tcgcgctgga gcgttcgcac ctcgcctgac
    34561 ccaccctgct ggtagccgct tccaggctcc agcagtggag aacggagagc tctccgcgtc
    34621 tgcggaccga ccttgccgtc gaccgctatg ccctgactct gttgaaagcg tgtgaccgcc
    34681 cgggtcgtga gcaggccgta cagcccgtcg atcggacccg gatcgaagcc cgcgcgtgat
    34741 agccggcgtt gcaccgagcg cacctcgcct gaaccacctt ctcggtagcc gcttcccagg
    34801 acgagcacgc tctcggacgc atgcgcgagc cgagtcactt cagcgccggt gtgcgatgac
    34861 cggccgtttg ggctctgggc ctcggcctgc catggcgcca gcgcaagcgc tgcggcgagc
    34921 accaggagga ggactcccgg gtgcgcgcgg agcgcgaagt gatggcgtac tgatgacatg
    34981 gaacttccct ttcgaatacg agcgaacatt tgggggcgcc gccggcgcgc gcctatggcg
    35041 cccggcgacg ggcctaccgg gccgccgcgc gaaagcgcgc agcgacgacc ggcctagcta
    35101 ccgccggccg cggtcagcca ggtcgctcgg aaagggcgat gaaggggacc ggccggaagc
    35161 aggccgggtc cacggagagc cgccggcgaa gggccggggc tgtaagggca aagagaatga
    35221 ggaacgccgc gatgccgccg aacatgagac cgctcggcga ggcggcgccg gacccctgcg
    35281 cggcacccgc agggaagggg ctgtccggat gcgcggggcg ggaggcgcgc gacacggcgt
    35341 cacgcgtcga gggcgcggtg gcggcgatcg ggcttgtcgc tccggcgagc atggacgccg
    35401 cggtgcggcc gagcttcgcg ggcgacacgg atccgtgtgc cctgcgctcg tcgcgcgtgg
    35461 ccccagccgg cgcgaggacg ctcggcgtgt gcgagagaag cgactgtgga agcggcaccc
    35521 gcgtgagtgc ctggccgacg gacggagtgg ccgggtcggt cggcgccggc gtgcgctcga
    35581 tcgcgggcgc tagcgcggac ggtggcgggg ccggcgggcc cggaaggacc gtgcccagga
    35641 gaggcgccgt cgtttcgacc actggtgcga gcggagcgag gagcgagtct gttggcggga
    35701 ccccggtccc aaggagcggc gcggtcgtcg tgtccattgc accgagcagc gaggtcgtcg
    35761 aatcgagcag aggcgacacg gcgccatcca gcggcggcgt cgtcgagtcg agtgccggga
    35821 cgaccgtcgt atcgagcagc ggcgtcgtcg aatcgagcac cggccccacc gcgccatcca
    35881 ccaacgacgt cgtcgtatcg agcagcggga cgaccgccgt atcgagcagc ggtgtcgtcg
    35941 tatcgaccaa cggcgccacc gctccatcca ccagcgaagt cgtcgtatcg acgaccggcg
    36001 ccaccgcttc atccaccagc gaagtcgtcg tatcgaccac cggggccaac gcgccatcca
    36061 ccaacgacgt tgtcgaattg agcaccggcg ccactgttcc atcgacgagc gacgtcgtcg
    36121 tgtcgaccag cggcgcggtc gccgagtcga gcgtcgaagc aggcgagcca ccactcgggc
    36181 cacccgagcc agtcgtcgag cctgccgtcg actccaccga gtcggccgtc gagttcgccg
    36241 tcgaaccggc cgagccactc gtcgagtccg tggtcgacgt gaccgtgtcg ccgatcgagt
    36301 cgatcgtctg ctcggccgag gtgacgccgg agtcgacggt tcgactgacc gagtcggacg
    36361 tcgaggccgc ggtgttcgtg acggcgctcg tcgtatcgga aaccgtcgac gtggccgtat
    36421 tcgtcacgtc ggacaccgcg ttcgtcacgc tcgacgtgtc cggcgagctc ggaacgctcg
    36481 tggtgttgcc ggacggcgcg ctcggcgtgg acggcgcgct cggcgtggac ggcgtgctcg
    36541 gcgtgttggt ggacggcgta cttggtgcgg acggcgcgct gggcgtgtcc gtcgacggcg
    36601 cgctcggagc agacggcgtg gcgggcggcg cggcttgcgt gaccgagtcg accgtcgagt
    36661 tgaccgtcgc ggtcgctccg tcgaccacat tcgtgaccgt cgcccctgtg tcgagagcca
    36721 gcgccacgct tggcagaagc ccgagcccca gcagcgcgac ggcgagcacg cagagcgatc
    36781 tccgaatcgc gtaccacctg ctgggtgacg aagagctagc cgagctcatc cgtggaaggc
    36841 ctcccgttct ccacgcgcgc gtggggacgg agtgccccgg tcaacaggcc cgaaacactc
    36901 gcgaaagcgg taaatcgact cgcctcacga cgcactacga aagtgccctg cgtgcaggca
    36961 tatttgacgc acgcgtcacg gacgcgccca cgcgcaatcc cggcgcatgg gcacaagtgg
    37021 ccggtggctt tgactcaggc cgcggcgggc tccgcgacgc ggcgtttcgg accgccgttg
    37081 cggctcggcg cgcgccgctt cttgggcgtg acgagccccg cgaggaaccg tccggtgtga
    37141 gagccctcgg tgccggcgac ctcctcgggc gtgccctgag ccaccacgag gccgccctcg
    37201 tcgccaccct ccgggcccat gtccacaatg cggtcagcgg tcttgatcac gtcgaggttg
    37261 tgctcgatca cgaccaccgt gttgccctga tcgaccagcc gctcgagcat ctcgagcagc
    37321 cgctgaacgt ccgcgaaatg aagtcccgtg gtcggctcgt cgaggatgta gagcgtgcgg
    37381 cccgtcgcca ccttcgcgag ctccgtggcg agcttcacgc gctgcgcttc gccgcccgag
    37441 agggtggtgg ccggctggcc cagccggatg tagtcgagac cgacgtcgtg aagcgcctga
    37501 aggcgccgct tgatcttcgg gatgtgccgg aagaagtcga gcgcctcctc gaccggcatg
    37561 tcgagcacgt cggcgatcgt cttgctcttg aaacggacct cgagcgtctc gcggttgtat
    37621 cggcgcccgt ggcactgctc gcacggcacg tacacgtcgg gcaggaagtg catctcgatc
    37681 ttgatctggc cgtcgccgcg gcagacctcg cagcgcccgc ccttcacgtt gaacgagaac
    37741 cggccgggct tgtaaccgcg cgcccgcgcc tcctgcgtgc gcgagaacag ctcgcggatc
    37801 gagtcgaaca cgccggtgta ggtggccggg ttggagcggg gcgtgcgccc gatcggcgac
    37861 tggtcgatgt tgatgatctt gtcgacctgg tcgagcccgg tcacgcgccg gtgcgcaccc
    37921 ggacgcagct tcgcccggtg caggcggttg gccaccgact tgtagagaac gtcgttgatc
    37981 agcgtggact tgccggagcc agacacgccc gtgacgcagc agaacacccc gagcgggatc
    38041 ttcgcgttga tgccgcgcag gttgtgctgg gtggcgccct cgatctcgat gtagccagac
    38101 ggccgcctgc gcttggccgg gacctcgatg gtgcggctgc cggcgagaaa ctggccggta
    38161 gcggactcct tcacgttcat gacgtcgtcg ggggtgccct gcgcgacgag gtagcctccg
    38221 tgctcgccgg cgcccggccc gaggtcgacg atgtgatccg cggcccgcat cgtgccctcg
    38281 tcgtgctcga cgacgatgac cgtgttgccg aggtcgcgca gccgctccag cgtccggatc
    38341 agccgctcgt tgtcgcgctg gtgcaggccg attgacggct cgtccaggat gtacaggacg
    38401 cccaccagcg acgacccgat ctgcgtggcg agcctgatgc gctgagcctc accgccggac
    38461 agcgtggctg cggcgcgctc cagcgacagg tagccgacgc ccacgttgtc gagaaaccgc
    38521 aggcgctcgt cgatctcgcg caggatcagc cgtgcgatct gccgctcggt gttcgacagc
    38581 tcgagcgctt cgaaccactc gatcgcgcgc cgcgcggaca tgcgggtgag ctcatggatg
    38641 ccgagcccgc caaccttcac cgccaggctc tcgggccgaa ggcgcgcgcc gtggcactcg
    38701 gggcacggcc gcagcgtcat gtattcctcg atcttctccc gcgagtagtc cgagtcggtc
    38761 tcgtggtagc ggcgctcgag gttcgtgatg atcccctcga agctcgtggt gtacgagcgc
    38821 cggcgcccgt agcggttctt gtagctgatg tagagccggt cgccgttcgt gccgtacagg
    38881 aagcactcct ggacctcctc ggggaggtcc tcccaggggg tctcgaggtc gacctcgtaa
    38941 cggtcggcga tcgcctgcgt catctgctcg tagtagttcg acgcggtggt ggaccacggc
    39001 agcagcgcgc cctccgccag cgacagcgac gggtcgggca ccaccaggtc cggatcgatc
    39061 tccatctgcg acccgagccc ggtgcagcgg gggcaggccc cgtgcggcga gttgaacgag
    39121 aacatccgcg gctcgagctc gggcatgctg gtgccgcagt tcaggcaggc gaagcgctcc
    39181 gaatacgtgt ggacgacacc ctcggacccg tccgacggga ccgtctcgat ctcgacgatg
    39241 ccctccgcga gggccaccgc cgtctcgacc gagtcggcca gccgcttgcg gaggtccggc
    39301 cgcatgacga gccggtcgac cacgacggcg atgtcgtgct tgaacttctt gtcgagcgcg
    39361 atgtcctcct cgagccgacg cagctcgccg tccaccttca cgcgcgcgta gccctcggcc
    39421 cgcaggtcct cgaagagctt gccgtactcg cccttccgcc ccctcactac gggcgcgagc
    39481 accatgaagc gctcgccctc ctggagcgtc atcacctggt cgacgatctg ctcggccgac
    39541 tgggcgacga tcggctgccc gcagttgtag caatgcgggt gacccacccg ggcccagagc
    39601 aggcgcaggt agtcgtagat ctcggtgacc gtgccgaccg tggagcgcgg gttgcgcgaa
    39661 gtggtcttct ggtcgatgga gatggccggt gacaacccct cgatcgaatc cacgtcgggc
    39721 ttgtccatct gccccaggaa ctggcgcgcg tacgccgaga gcgactcgac atagcggcgc
    39781 tggccctcgg cgtagatcgt gtcgaaggcg aggctggact tgccggatcc cgacagcccg
    39841 gtgatgacta cgagggcgtt tcgcgggatc tcgaggtgga ggtccttgag attgtgctcc
    39901 cgcgcgcccg agatgaccaa ctcggatcgc tgactcactt ctttcagtct aggcaccggg
    39961 tcggtcgaac acgtgttcgg ttcccgctct ttacggatat ccgcccccca gctgacgagg
    40021 cggccccatt ggtggtggct cgcgtagagg cgaccatgcc tccacagccc cttcagccac
    40081 tccaagagga cgacgatgac cgaccgcacc cgccacgacg agatcaccca cagccgagcc
    40141 ggcgacgtgt tcgtgaatcc cgttaccggc gagcgcgccg tgatcgtgtg cgggaccgcc
    40201 gacagcactg acggcagcct ctcggtccac ctcttcgtcg cgccgggtgg agcggttgcc
    40261 gccgagcatt atcaccctca gctcgtggaa cgcttccgcg tgctgtcggg ccacctcgcc
    40321 gtgtccgtcg aggggcgccg cagccacatg ggcccgggcg acgagctcag cattccgccg
    40381 ggcgtgatcc acgactggtg gaacgccggc gagactgagg ccgaggtgct cgtggaggtc
    40441 actcccgggc ggcgcttcga gctcatggtg ggcagcctgt tcggccttgc acgcgacggg
    40501 aaggtcaacg caaaggggat gccgaacctc ctccagctcg cggtcatggc gagcgagttc
    40561 gacgacaccg tccggttcgt gaagccgagc ccgatcgtgc agaagcttct cttcgccgtg
    40621 ctcgcgccgc ttggccgcct gctgggctac cgcccttact acgcggagta tctcgtcccc
    40681 gccgagcaca tcgagccgga gcgctccgtg ctcgacatgg tccgctccca ccacgccgcg
    40741 ccgaggaccg ccttggcctg agccatgctg gagcgcgtgg acacagtcgt cgtgggcgcg
    40801 ggtcaggctg ggctggctgc cagcttccgc ctcgagcgcg ccggcgtgga ccacgtggtg
    40861 ctcgagcgtg gccgcgtggg cgagtcctgg ctctctcagc gctgggacga gttcaggctg
    40921 aatacgccga actgggccaa cctgctcccc ggcatggacc tgcgcggcgc cgatccccac
    40981 gccttcgcga gcgcgcggga gttcgccgcc cgactcgctc ggtacgtgcg cgtgaaccgg
    41041 ctcccggtgc tcaccggcgt gagcgtcatg gggctcgagc ctgacggcga acacggctgg
    41101 cgcgtgacca ccgacgccgg tgactacctc tgccgagacg tggtactcgc cgccggctgg
    41161 ctgaaccgtc cccgcttccc tgccgtggcg ctcaccgtgc cgctgtcgat cgaacgcatc
    41221 cacaccgccg cctaccgcaa tgcctcgcag cttcccgacg gtggcgtgct ggtcgtgggc
    41281 tccgggcagt ccggcgtgca gatcgccgag gacctgctcg gatcgggccg cagcgtctag
    41341 cccgcgcgcg caggcttcgg cctcgatgcg ccgcgcgaga tcgacgcccg ctccattgga
    41401 tcggtgattt gggcgaccgg cttcagcccg gacctccggt ggctggcgcc ttcgctgctc
    41461 gagcgcgagg gcttgcacgt gctcgggcgc gcgtggcttc gctcacgcaa gtccggcatg
    41521 gtctacggcg ccgccgagga cagcgcggcg atcgtggcgc ggatcacggg tgggcgtgcc
    41581 gtcgagatgg gtcgacgacg gttggctcat caagctcctg ctgccgagca cgcggttcag
    41641 caacctcaac tggtcgcgta gcgagacggg cgacgcccgg ccgggtgtcg tcgatttggg
    41701 tcgatagtgg ccgctaagta acgacgctca gcggtttctg tcgatttcag cccagtggcg
    41761 ggccttcgcc cgagcagccg ttattgctgg tgtaaacgag gcgggcaatg acgcgggagg
    41821 cgggcgacca acgccgcctg acgtccgcac cggcggccat catcgccccc gtgagggtgc
    41881 tcgacttcct cacgatgatg ctcgccaccg tgcggggcag gccgccccgc cagccgcgcg
    41941 cgccccgtcc gataccctcc aagccgtctt ctacgggagg aagcttggac ctcgaccgca
    42001 ctcgcatcga acgcaaggac ttcagcgtcg tccgaagggg ctacgacccg tcggaggtgg
    42061 atctacacct gcgcgacctg gccgacgccg tggccgagct gcgggcggag ctccggtcga
    42121 cacccgccgg tgtggcgggc gcagccgccg agcacgtgaa gacgatcgtc gaggccgccg
    42181 agaagagcgc tgccgagatc gagg
//
LOCUS       DASVXS010000152         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_656811, whole genome shotgun sequence.
ACCESSION   DASVXS010000152 DASVXS010000000
VERSION     DASVXS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656811"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF752_08480"
     CDS             <1..437
                     /locus_tag="VF752_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8855624.1"
                     /translation="KTTPKQVEAVLAERPKTKGAGNLRKVLRGELRVTLSKLEREFIK
                     ALKAHGLPLPITNRVTGAHRVDCYWPEHKLVVELDSYTYHQSRHAWEQDRRRERDARR
                     NGNEFRRFSHDDVLDRRALVLAEMRALTGSSPSRRRGTRAGS"
     gene            complement(388..696)
                     /locus_tag="VF752_08485"
     CDS             complement(388..696)
                     /locus_tag="VF752_08485"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX8855625.1"
                     /translation="MSDERPERQINIHTSPEIMAGHYANFANVSHSDYEFTITFARVD
                     HEVDEDEIPGVVVSRMNLSPRFMRELIDAMQDNYQRWQTREGIKNLPEFPGGETDSTQ
                     "
     gene            732..1142
                     /locus_tag="VF752_08490"
     CDS             732..1142
                     /locus_tag="VF752_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX8855626.1"
                     /translation="MRTIVLSVGRLRPPFADDIQHYQKLLARHARLELLELSEEEKVE
                     GRIPERAFVVLLDSSGRTFDSVGFSKFLEERRQSRQDLCFVVGGPRGLVTVPNCDLRL
                     SLGPMTLPHQLARVVLLEQLYRAHKILAGEPYHY"
     gene            1144..>2105
                     /gene="argS"
                     /locus_tag="VF752_08495"
     CDS             1144..>2105
                     /gene="argS"
                     /locus_tag="VF752_08495"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8855627.1"
                     /translation="MPETSNPLAELRGAVADAARELAGGNGRRPDPSFDRPPRPDFGD
                     YSSNAAMLIAGSVGEPPRAVAERLGELVSQRLGEAVERVEVAGPGFLNLFVSDEWWRQ
                     ALSSAVAAGDDYGSNVVDESEHVLVEFVSANPTGPITVASGRHAAYGDSLSRVLEFAG
                     HRVEREFYVNDYGSQIRRFGESIKARARGEEPPEDGYKGEYVTELAARIRDAADANPE
                     ELAEKGIELMLEGVSDTLERFRVHFDRFFSERSLHGQNAVQHALDVLEEGEHLYPHEG
                     AVWLRSTTFGDDKDRVLMRSTGDWTYLAADIAYHQHKLERNYDRA"
BASE COUNT          341 a          736 c          709 g          319 t
ORIGIN      
        1 acaagaccac tccgaagcag gtcgaggccg tcctcgcgga acgcccgaaa actaagggcg
       61 ccggcaatct ccgcaaggtc ctccgaggcg agctgcgcgt cacgctcagc aagctcgagc
      121 gcgagttcat caaagcgctc aaagcacacg ggcttccgtt gccgatcaca aaccgcgtca
      181 ccggtgccca ccgagtggac tgctactggc cggagcacaa gctcgtcgtc gagctcgact
      241 cctacacgta tcaccagtca cgccacgcct gggagcagga ccgccgccgc gagcgcgacg
      301 cccgcagaaa cgggaacgaa ttccggcgct tctcgcacga cgacgtgctc gaccggcgcg
      361 cgctcgtgct cgcggagatg cgcgcgctta ctgggtcgag tccgtctcgc cgccggggaa
      421 ctcgggcagg ttcttgatcc cctcccgggt ctgccagcgc tggtagttgt cctgcatcgc
      481 gtcgatcagc tctcgcatga accgcggcga gaggttcatc cgcgacacga ccactcccgg
      541 gatctcgtcc tcgtcgacct cgtggtccac ccgcgcgaag gtgatcgtga actcgtagtc
      601 cgagtggctg acgttcgcga aattcgcgta gtggccggcc atgatctcgg gggaggtgtg
      661 gatgttgatc tggcgctcgg gccgctcgtc cgacacgcgg ttagtatcgc agccggaggt
      721 cgccgaggca gatgcgaacg atcgtccttt ccgtcggccg acttcggccc ccattcgcag
      781 acgacatcca gcactaccag aagctgctgg cgcgccatgc ccgcctcgag ctgttggagc
      841 tgagcgagga ggagaaagtc gagggccgca tccccgagcg cgccttcgtg gtgctgctcg
      901 actcgagcgg aaggaccttc gactcggtcg gcttcagcaa gttcctggag gagcgccgcc
      961 agtcgcgcca ggatctctgc ttcgtagtcg ggggcccgcg cgggctcgtc accgtcccca
     1021 actgcgacct gcggctctcg ctcgggccga tgacgctccc gcaccagctc gcacgggtgg
     1081 tgctgctcga gcagctgtac cgcgctcaca agattctcgc gggcgagcca taccattact
     1141 gacgtgcccg agacctccaa cccgctggcc gagctgcgcg gggcggtggc ggacgcagcc
     1201 cgggagcttg ccggcggaaa cggccgccgg cccgatccga gcttcgaccg accgccgcgc
     1261 ccggacttcg gcgactactc cagcaacgcc gcgatgctca tcgccggctc ggtcggcgag
     1321 ccgccgcgcg cggtcgccga gcgcctcggc gagctcgtga gccagcgcct gggggaggcg
     1381 gtggagcggg tcgaggtggc cggcccgggc ttcctcaacc tgttcgtgag cgacgagtgg
     1441 tggcgccagg cgctgtcgtc ggccgtcgcc gcgggggacg actacggctc gaacgtcgtg
     1501 gacgagagcg agcacgtgct cgtggagttc gtgagcgcta atcccaccgg tccgatcacc
     1561 gtcgcctcgg ggcgccacgc cgcctacggc gactcgcttt cccgcgtgct cgagttcgcg
     1621 ggccaccgcg tggagcgcga gttctacgtg aacgactacg gcagccagat ccgccgcttc
     1681 ggggagtcga tcaaggcgcg cgcccgtggc gaggagccgc cggaggacgg ctacaagggc
     1741 gagtacgtga cggagctcgc cgcgcgcatc cgcgacgcgg ccgacgcgaa ccccgaggag
     1801 ctcgccgaga aggggatcga gctgatgctc gagggcgtga gcgacacgct cgagcgcttc
     1861 cgcgtgcact tcgaccgctt cttctcggag cgctccctcc acggtcagaa cgcggtgcag
     1921 cacgcgctcg acgtgctcga ggagggcgag catctctacc cgcacgaggg cgcggtctgg
     1981 ctgcgcagca ccaccttcgg agacgacaag gaccgcgtgt tgatgcgctc gaccggcgac
     2041 tggacctacc tcgccgccga catcgcctac caccagcaca agctcgagcg caactacgac
     2101 cgcgc
//
LOCUS       DASVXS010000153         6855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_727834, whole genome shotgun sequence.
ACCESSION   DASVXS010000153 DASVXS010000000
VERSION     DASVXS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727834"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..864
                     /locus_tag="VF752_08500"
     CDS             439..864
                     /locus_tag="VF752_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX8855628.1"
                     /translation="MTVVGPPADWRDLGRQTDAIVEACAERRLTLLDVQVEHELSGVA
                     PLERRGLRQALARVSSGTAGCFVVADLNRITQGVTDLGPVLEWLSRSDTRLIAAAQRI
                     DTADRRSRARVEWLIAMSCIHRELARGFPAERRQAGVAV"
     gene            complement(921..1163)
                     /locus_tag="VF752_08505"
     CDS             complement(921..1163)
                     /locus_tag="VF752_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8855629.1"
                     /translation="MAGFVGKALMPGDDPGGFFVTILLGLAGAALGWLIFTAGLGIGD
                     EDVFDLGGIVSAIIGVVILLGLYRWWVGRRAGPRTA"
     gene            1293..2921
                     /locus_tag="VF752_08510"
     CDS             1293..2921
                     /locus_tag="VF752_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8855630.1"
                     /translation="METYDYVIVGAGSAGCVLANRLSADQGIRVLLLEAGGSDRKLNI
                     RIPVGFAKQFRSDLDWNYVAEPEQNLIGRSIYLPRGRSLGGSSSMNAMIYVRGHRTDF
                     DGWADAGATGWDYDSVLPYFKRSEHNERLHNEFHGQDGPLNVADPVWQSSLAPMFIES
                     AAQAGIGRTDDFNGAEQDGAGLMQLTQKRGRRWSAADAFVHPVKKERSNLKIVTGAQA
                     RRVLMEDGRATGVEFLHEGRAVQARALEEVIVSAGAFNSPQLLMLSGIGPADELRSAG
                     VEPLVDSPHVGRHLMDHPMTTVTFECKEASTLLDATNPKYLVQYVAGRGAGKLSSNVA
                     EAGAHVRVTDGVSAPDVQLLFGAAYYFDNGFRSYPNPAFTIAQCFLAPVSEGSVRLRS
                     ADPLAKPAIHLGWLGERSEIEAMIGAIRLSREIASSGPLADAVVMNIDPGPGVASDEQ
                     LEAWIRAEAQHEYHAACTCRMGREGEGVLDEQLRVRGVESLRVVDASSMPRITHGNTN
                     APVIMLAERASDLILGRKPLTPERRAAPQPAVAS"
     gene            complement(2918..4687)
                     /locus_tag="VF752_08515"
     CDS             complement(2918..4687)
                     /locus_tag="VF752_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8855631.1"
                     /translation="MQGKGFAAALVAVLALALVPQASAATVSKCNVPIEMSDGTTLYA
                     NVWLPSDSGRFPTLLQTTGYNKDVFYGGNCMPPDSALVDAGYAEILLDDRGTGASEGR
                     WDSWGERTQQDYSEVLDWLQRQPWWNGHAATFGASYMAITSLLTAEADAKRVADGKPR
                     AIDAIWADVPMADAYRDVTFHGGAVDSGFMPFWLGLTTTLSDIPPSTIFSDPASALAT
                     YAGHLANSFDFAARKLLEVSLGEDAAYDSDFYRLRSPVERIKSLKIPVAWTGGWWDIF
                     QRGEPLLFEQMTNSPNKHFWMSPGYHIATGGTNWDAQGIGSKEEVMTRWFDRWLRGDR
                     NGVDSLPRVNLFTMGRDQWRHDGTWPLPGTKYTPFYLGDNKSLSPAPATTPGADTAPL
                     MPASSPCSRLTSQWTAGLAPGGPCETDGRTFEATALTYTTPPLEKDTEVTGLISADLF
                     AELNGATDATLVAVLSDVDPSGASNQITSGFLLASQRALDRSKSTFGPGGVMIRPYHP
                     FTKGSQQPVTPNDPTEYRIEIYPTSNVFKAGHRIRLTIATANTPGTLTPLPDTLNELG
                     GQIRLLHGPAYKSSVQLPLAPAP"
     gene            4830..6227
                     /locus_tag="VF752_08520"
     CDS             4830..6227
                     /locus_tag="VF752_08520"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEX8855632.1"
                     /translation="MAVLKPDPTFYPSPRLAAEAPAEELAYVVTLNTGTNGDAKPDAL
                     NVVDLKPSSSSYGGLIGRLDMPNVGDELHHFGWNACSSALCPWAPHPHVERRYFLVGG
                     VRSSRIHVIDVKSDPVNPELVKVIEPDEFAKRTGYSRPHTVHCGPDGLYVSGLGAPDG
                     NGPGGVALLDHDDFSPLGQWEVDRGPQELSYDVWWNIGYDTLVTSEWGTPKMFEDGVN
                     PELLLAGKYGHHLHIWDLKGRRHKQAIDLGPEQQMVLELRPAHDPRRPYGFVGVVVST
                     ADLSASVWLWERQPDGSVGVKKVIEIPAEAAEVEQLPPAVQPFGAVPPLVTDIALSLD
                     DRYLYVSCWGTGELKRYDVSDPHNPKEAGSVRLGGMVQRSPHPASGPVNGGPQMVEVS
                     RDGRRVYLTNSLYSTWDAQFYPDGIDGWMVKLDSEPDGRFELDPDFFIEFDGERPHQV
                     RLQGGDASSDSYCFP"
     gene            6224..>6855
                     /locus_tag="VF752_08525"
     CDS             6224..>6855
                     /locus_tag="VF752_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855633.1"
                     /translation="MSHDTGLWLTLIGLGAYHGINPAMGWLFAVSNGLQHGERRAVVR
                     SLLPIALGHEMSIALVAALVLGAESFIDPSSLHIGAAVVLFAFGIFRFVKPRAHWRWT
                     TMRVNNRELTLWSFLMSTAHGAGLIVAPVLIGLGGASAATAASNAHDHADVAMTSDVS
                     WLSSGVGVMLHVAAMLLVMGVVAVVIYEKVGLGVLRRAWVNTDHLWAGAF"
BASE COUNT         1029 a         2323 c         2436 g         1067 t
ORIGIN      
        1 agcgccgcgt cctcgggcgg cggcgggatc agcctgatcg cgttgcccgc cgtgctcgcc
       61 gcccttctgc tcgcgctctt cgagctactt cgcatcgccc cggcccgctg gcggtcggtg
      121 gcactcgtct cactcctcga gcggcctggc tgacgccctg acgggctgaa ccagggtccc
      181 cgggcacgct cctcgcagcc cggaggacag atctgctttt gaggagttgg agcaatgcaa
      241 caacaacaca acgccttcac cggcctggca ggggtcctca gagatctgtc aggagcctca
      301 agtccgaggc gagcagggag gcgcgcctca ccagggcccg tcgcgaacgg cttcccacgg
      361 aagcggatgc ttcccctcga gctaccggct cgaggggcgc ggcgggctcg ccgtcggcgg
      421 gcaccggcga tcgggtacgt gacggtcgtc ggaccgccgg ccgactggcg agaccttggg
      481 cgccagacgg atgcgatcgt cgaggcctgc gccgagcggc gcctcacgct gctcgatgtc
      541 caggtcgagc acgagttgag cggagtcgca ccgctcgaac ggcgggggct caggcaggcg
      601 ctcgcgcgcg tgtcatccgg cacggccggc tgcttcgtcg tggccgacct gaaccggatc
      661 acgcagggcg tcacggacct cggtccggtg ctcgagtggc tctcccgctc ggacacgcga
      721 ctgatcgccg cggcgcagcg gatcgacacc gctgaccggc gatcgcgcgc tcgggtggag
      781 tggctgatcg cgatgtcctg catccatcgc gagctcgctc gcggtttccc agctgagcga
      841 cgccaggcgg gcgtcgccgt ctaagcggca ggcgcaggca ggcgagggaa cgcctgcctg
      901 cgccgccggt ttacggcttg tcaggcggtg cgcggacccg cgcggcgccc gacccaccag
      961 cggtagagcc cgagcaggat caccacgccg atgatcgcgc tcacgattcc gccgaggtca
     1021 aatacgtcct cgtcgccgat gccaaggccg gccgtgaaga tcagccagcc gagggccgct
     1081 ccagccaggc ccagcaggat cgtgacgaag aagccgcccg gatcgtcgcc gggcatcaac
     1141 gccttcccga cgaagccggc caccagaccc aaaatcaatg caccaatcac cttcggaaca
     1201 cctccgtgga cgcgcggcgg gccatcacgt gtaatacccg caacaggccc cattcgttac
     1261 ccgccgtgcg tgccactaac ccaggaggaa ccttggagac ctacgactac gtgatcgtcg
     1321 gcgccgggtc ggccggctgc gtgctcgcga accggctgag cgcggaccag ggcatccgcg
     1381 tcctgctgct cgaggccggc ggctccgacc gcaagctgaa catccgcatc ccggtcgggt
     1441 tcgccaagca gttccgcagc gacctcgact ggaactacgt ggccgagcct gagcagaacc
     1501 tgatcggccg ctccatctac ctcccgcgcg ggcgctcact cggcgggtcg agctcgatga
     1561 acgcgatgat ctatgtgcgc ggccaccgca ccgacttcga cggctgggcc gacgccggcg
     1621 cgaccggctg ggattacgac tcggtgctcc cctacttcaa gcgctcggag cacaacgagc
     1681 gcctgcacaa cgagttccac ggtcaggacg ggccgctgaa cgtggccgac ccggtgtggc
     1741 agtcgtcgct cgcgccgatg ttcatcgagt ccgccgcgca ggcgggcatc ggccgcaccg
     1801 acgacttcaa cggcgccgag caggacggcg ccggcctgat gcagctcacg cagaagcgcg
     1861 ggcgccgctg gagcgcagcc gacgcgttcg tccatcccgt gaagaaggag cggtcgaacc
     1921 tgaagatcgt caccggggcg caggcgcgtc gcgtgctgat ggaggacggg cgcgcgaccg
     1981 gcgtcgagtt cctgcacgag ggcagggccg tgcaggcgcg ggcgctcgag gaggtgatcg
     2041 tctccgccgg cgcgttcaac tcgccgcagc tgctgatgct ctcgggcatc ggcccggccg
     2101 atgagctccg ctcggcgggc gtcgagccgc tcgtggactc gccgcacgtc ggccgtcacc
     2161 tgatggacca tccgatgacc acggtcacgt tcgagtgcaa ggaggcctcc acgctgctcg
     2221 acgcgaccaa cccgaagtac ctcgtccagt acgtggccgg gcgcggcgcc ggcaagctga
     2281 gctcgaacgt cgccgaggcc ggtgcgcacg tgcgcgtgac ggacggcgtg agcgcgccgg
     2341 acgtccagct gctgttcggc gccgcgtact acttcgacaa cggcttccgc agctacccga
     2401 atccggcgtt cacgatcgcg cagtgcttcc tggcgccggt gagcgagggc tcggtgcggc
     2461 tgcgatcggc cgacccgctt gccaagccgg cgatccacct cggatggcta ggcgagcgct
     2521 cggagatcga ggcgatgatc ggcgccatcc ggctctcgcg cgagatcgcc tcgagcgggc
     2581 cgcttgccga cgcggtggtg atgaacatcg acccggggcc cggtgtcgcg agcgacgagc
     2641 agctcgaggc ctggatccgc gccgaggcgc agcacgagta ccacgccgcc tgcacgtgcc
     2701 gcatgggccg cgagggcgag ggcgtgctcg acgagcagct gcgggtgcgc ggtgtggagt
     2761 cgctgcgagt ggtcgacgcg tcgtcgatgc cgcgcatcac gcacggcaac acgaacgcgc
     2821 cggtgatcat gctcgccgag cgcgcgtcgg atctgatcct cggccgcaag ccgctcacgc
     2881 cggagcggcg cgcggcgccc cagcccgccg ttgcgagcta gggagcgggc gccagcggca
     2941 gctgcacgct cgacttgtaa gcgggcccgt gcagcagccg gatctggccg ccgagctcgt
     3001 tcaaggtgtc gggcagcggc gtgagcgtgc ccggagtgtt cgcggtcgcg atcgtgagcc
     3061 tgatccggtg gccggccttg aacacgttcg acgtcgggta gatctcgatc cggtactcgg
     3121 tcgggtcgtt cggcgtgacg ggctgctgcg atcccttggt gaacgggtgg tacgggcgga
     3181 tcatcacgcc gcccggaccg aaagtggact tcgagcggtc gagtgcccgc tggctcgcga
     3241 gcaggaagcc ggacgtgatc tggttcgagg cgccggatgg atcgacgtcg ctcagcaccg
     3301 ccacgagggt cgcatccgtc gcgccgttca gctcggcgaa caggtctgcg ctgatcagcc
     3361 ccgttacctc ggtgtccttc tcgagcggcg gcgttgtgta agtgagcgcg gtcgcctcga
     3421 aggtgcgccc gtcggtctcg cagggcccgc cgggcgctag gcccgccgtc cactgactcg
     3481 tgagccgcga gcagggactc gacgccggca tgagcggcgc cgtgtccgcg ccgggtgtcg
     3541 tggcgggcgc ggggctcagc gacttgttgt cgccgaggta gaaaggcgtg tacttcgtgc
     3601 cgggcagcgg ccaggtgccg tcgtggcgcc actggtcgcg gcccatcgtg aagaggttca
     3661 cccgcgggag cgagtcgacg ccgttgcggt cgcccctcag ccagcggtcg aaccacctcg
     3721 tcatgacctc ttccttggac ccgatcccct gtgcgtccca gttcgtgccg ccggtggcga
     3781 tgtggtagcc gggactcatc cagaagtgct tgttgggcga gttggtcatc tgctcgaaca
     3841 gcagcggctc gccgcgctgg aagatgtccc accagccgcc ggtccaggcg acggggatct
     3901 tcagcgactt gatccgttcg acgggcgagc gcaggcggta gaagtcagag tcgtaggcgg
     3961 cgtcttcgcc gagcgagacc tcgagcagct tgcgggcggc gaagtcgaag ctgttcgcca
     4021 ggtggcccgc gtacgtggcg agcgcggagg cggggtcgct gaagatcgtc gacggcggga
     4081 tgtccgacag cgtggtcgtg aggccgagcc agaacggcat gaagccggag tcgacggcgc
     4141 ctccgtggaa ggtcacgtcg cggtaggcgt cggccatcgg cacgtcggcc cagatggcgt
     4201 cgatcgcgcg cggtttgccg tcggccacgc gcttggcgtc ggcctccgcg gtcagcaggg
     4261 aggtgatcgc catgtaggag gcgccgaagg tggccgcgtg gccgttccac cagggctggc
     4321 gctggagcca gtcgagcacc tcggagtagt cctgctgggt tcgctcgccc cacgagtccc
     4381 agcggccctc gctggcgccg gtgccgcggt cgtcgagcag gatctcggcg tagccggcgt
     4441 cgaccagcgc ggagtcgggc ggcatgcagt tgccgccgta gaagacgtcc ttgttgtagc
     4501 ccgtggtctg gagcagcgtg gggaagcggc ccgagtcgga cggcagccag acgttggcgt
     4561 agagcgtggt gccgtcgctc atctcgatcg gcacgttgca cttcgacacg gtcgcggccg
     4621 acgcctgcgg caccagcgcg agcgcgagga cggccactag cgccgccgcg aagcccttcc
     4681 cctgcatgac ttccccctgt cgtcgcgcgt cttataacaa gcggcgcttc atgcaggcgc
     4741 cggttctggg cgcaatcgcg ctaaagacgg gcaactttgc ggtggtacgc tgcgccgcac
     4801 ccttgcaccc gggactcgcg aggtatcgca tggccgtcct gaagccggat ccgaccttct
     4861 acccgtcgcc gcgcttggcg gctgaagctc cggcggagga gctggcctac gtcgtcaccc
     4921 tcaacacggg cacgaacgga gacgcgaagc ccgacgcgct gaacgtcgtc gacctcaaac
     4981 cctcgtcgtc gagctacggc gggctcatcg gccgactcga catgccgaac gtgggcgacg
     5041 agctccacca cttcggctgg aacgcctgct cgtcagcgct gtgcccatgg gcaccacatc
     5101 cgcacgtcga gcgccgctac ttcctggtcg gcggcgtgcg ttcctcacgg attcacgtca
     5161 tcgacgtgaa gagcgaccca gttaaccccg agctcgtgaa ggtgatcgag ccggacgagt
     5221 tcgcaaagcg caccggatac tcccgcccac acaccgtcca ctgcggcccc gacgggctct
     5281 acgtgtcggg actcggcgcg ccggatggca acggcccagg aggcgttgcg ctgctcgacc
     5341 acgacgactt ctcaccgctc ggccagtggg aggtcgaccg cgggccgcag gagctctcgt
     5401 acgacgtctg gtggaacatc ggctacgaca cgctcgtcac gagcgagtgg ggcacgccga
     5461 aaatgttcga ggacggcgtg aatcccgagc tgctgctcgc cggcaagtac ggccaccacc
     5521 tccacatctg ggatctcaaa ggtcgccgcc acaagcaggc gatcgacctc gggcccgagc
     5581 agcagatggt gctcgagctg cgtcccgcac acgacccgcg caggccgtac ggcttcgttg
     5641 gcgtcgtggt ctcgaccgct gacctgagcg cgtcggtgtg gctgtgggag cgccagcccg
     5701 acggcagcgt gggtgtgaag aaggtgatcg agatcccggc cgaagcggcc gaggtcgagc
     5761 agcttccgcc ggcagtgcag ccgttcggcg ccgtgccgcc gctcgtcacc gatatcgcac
     5821 tgtcgctcga cgaccgctac ctctacgtgt cgtgctgggg caccggcgag ctgaagcgct
     5881 acgacgtgag cgacccgcac aacccgaagg aggcgggctc ggtgcggctc ggcgggatgg
     5941 tgcagcgctc gccgcaccca gcgtccgggc cggtgaacgg cggaccgcag atggtcgagg
     6001 tctcgcgcga cggcaggcgc gtgtatctga ccaactcgct gtactcaacc tgggatgccc
     6061 agttctaccc ggacggcatc gacggctgga tggtgaagct cgactccgag ccggacggcc
     6121 gcttcgagct tgacccggat ttcttcatcg agttcgacgg cgagcgcccg catcaggtgc
     6181 gcctgcaggg cggcgacgcg tcgtccgaca gctactgctt cccgtgagcc acgacaccgg
     6241 cctgtggctg accctgatcg ggctcggcgc gtatcacggg atcaatcccg cgatgggctg
     6301 gctgttcgcg gtatcgaacg ggttgcagca cggtgagcgg cgggcggtgg tgcgatcgct
     6361 cttgccgatc gcgctcgggc acgagatgtc gatcgcgctg gtggcggcgc tggtgctcgg
     6421 ggccgagtcg ttcatcgacc cgagctcgct gcacatcggc gccgcggtcg tgctgtttgc
     6481 gttcggcatc ttccgcttcg tgaagccgcg cgcgcactgg cgctggacca cgatgcgcgt
     6541 gaacaaccgc gagctgacgc tgtggtcgtt tctcatgtcc acggcgcacg gcgcggggtt
     6601 gatcgtggcg ccggtgctga tcgggctcgg cggtgcgtcg gccgccaccg ccgcgtcgaa
     6661 tgcacacgat cacgccgacg tcgcgatgac gagcgacgtc tcgtggctct cgagcggcgt
     6721 cggcgtgatg ctgcacgtgg cggcgatgct gctcgtgatg ggcgtcgtcg ccgtcgtgat
     6781 ctacgagaag gtcgggctcg gcgtgctccg gcgggcgtgg gtgaacaccg accacctgtg
     6841 ggccggcgcc ttcat
//
LOCUS       DASVXS010000154         5376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1315330, whole genome shotgun sequence.
ACCESSION   DASVXS010000154 DASVXS010000000
VERSION     DASVXS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315330"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF752_08530"
     CDS             <1..183
                     /locus_tag="VF752_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855634.1"
                     /translation="AVPSSQLSPNSPRPVMAVAVLAAAAQVALMAAARRPRLAPGPVP
                     APEPALELAQALGNAA"
     gene            215..649
                     /locus_tag="VF752_08535"
     CDS             215..649
                     /locus_tag="VF752_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8855635.1"
                     /translation="MSSERKKQLVRRLYEEAWGRGELAVIDELFADEYTRHDLRPMAA
                     RPGPAGQRQIAEDFRTAFPDIEMTVDLLIADGDYVVARWTAEGTNTGPWGGGGQATGR
                     RARISGVNIFRFEGERVAELWNHRDDLGMMEQLAAPIYSGGA"
     gene            complement(646..1260)
                     /locus_tag="VF752_08540"
     CDS             complement(646..1260)
                     /locus_tag="VF752_08540"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX8855636.1"
                     /translation="MNDPRRRSFYERSVHAVARDLVGCTIRHGDTSGRIVEVESYHQE
                     EPACHAYNGLTPRTEVLFGPPGHAYVYRSYGIHALLNAVAEEEGLGAAVLIRALEPID
                     GVDLMRLRRRMDRVDDLCSGPGKLTQALGIGLDLNGTPITDGRGPIEFLPRPADSRPP
                     EVVLGERIGITRAVDLPWRFCDATSTSVSRPRPPAMRASARRAA"
     gene            complement(1257..2627)
                     /gene="argH"
                     /locus_tag="VF752_08545"
     CDS             complement(1257..2627)
                     /gene="argH"
                     /locus_tag="VF752_08545"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX8855637.1"
                     /translation="MSRFSEPQDPVFQALNSSVSFDYRLAPYDIEQSRAHVRMLGRSG
                     VIPEADAAEIERGLELVQREIDDDSFEVKPGDEDVHMAIERRVTELIGPVGGKMHTAR
                     SRNDQVATDATLFVRAHALRARDAILGLMGVLETLAERHIDWPMPGYTHLQRAQPVYL
                     SHHLLAYFWKLRRDFHRFGFVLSTTDELPLGAGALAGVNFSTDRMSVAQDLGFPAIAE
                     NSIDAVSNRDFVIDYVSAAATCATHLSQLGAEIVLWSSEEFRFCEVSDAFASGSSIMP
                     QKKNPDAAELLRAKAPRVAAHLVGLHGVLHGLPLTYNKDLQEDKEHLFDAVDTLELAL
                     AAATGVLRDIRFNRERLAEAASDEFLAATDIADMLVTRGVPFREAHGIVAGIVREALE
                     SGRSLSDFSDDELRGISEQLVPGFHELLGERAWLESKVSEGGTALSRVKEQLAQARHV
                     LEEASA"
     gene            2677..3657
                     /locus_tag="VF752_08550"
     CDS             2677..3657
                     /locus_tag="VF752_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8855638.1"
                     /translation="MRALVTGATGKIGNALVHLLAERGHEVRALLRDPVRAAPHLPAG
                     AETVQGDVTDPGSLPQAVEGCDVVFNAMGLPEQWLADENDFHRVNALGSENVTKAARA
                     AGVPRFVHTSTIDVFHAERGARFDESNVADYPKGTPYERSKQEAERLVLAAAGDGLEV
                     VIANPSAVYGPGPGGAGSSFEENLMRPIVKKRLPAVPPGGMGVTFNRGAADALLRIAE
                     RGRPGERYIVCDRHATMKELADSVVRIAGRGRVPPVLPVPVAKAMAGAGEALSRAIHR
                     PPLLPRGQLYFFLWDAAPDSSKVQEELGWEPTPLDEGLRVTLAEMDPPLD"
     gene            3650..4264
                     /locus_tag="VF752_08555"
     CDS             3650..4264
                     /locus_tag="VF752_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8855639.1"
                     /translation="MTDRLTTTIRPARPEDRDAVADLVYLTAGDAYDLFAGSREAAVE
                     LIRAAFERDGTDASRDVVTVAEVNGRVAGAMAAFPVAEGRDRSGRFLRMVLRMRPPWR
                     WGGVLRLWWAGTRGGPRPPFDSLYVDALATHPEFRRRGIALALLDEAERAARDRGLAQ
                     VALDTAAGNTAARLLYERAGFVLTEERRARGPIPALVGFAKRVA"
     gene            complement(4235..>5376)
                     /locus_tag="VF752_08560"
     CDS             complement(4235..>5376)
                     /locus_tag="VF752_08560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8855640.1"
                     /translation="LAKPLIGEREERLVLDTLRSGRLSLGPRLGEFEGAFAQRLGVPS
                     ASAVSSGTAGLHLAVRVAGIDAGDEVITTPFSFVASSNCVIYEGAKPVFRDIDPRTLN
                     IDPAAAAAAVGERTSGILPVHIFGYPVDMPAVEAIARERGLWIVEDACEALGAVHGDG
                     PAVGARGHLSTFAFYPNKQMTTGEGGMVVCPDAESKERIDSERNQGRAPDMGWLDHDR
                     LGFNYRLSDLACALGIAQLERLEELLAARSRVAALYGEALADIEGLELPCPDDGGDRR
                     SWFVYVVQLPHGVPRDDAIEELRARGIDSKPYLPAIHLMSFYRERFGHRPGEFPVCED
                     VAERSLALPFFPQLGEGEIERVASALREVVAQALTRSGDPLGEAH"
BASE COUNT          786 a         1869 c         1979 g          742 t
ORIGIN      
        1 gcggtaccaa gcagccaact cagcccaaac agcccgcgcc cggtgatggc ggtggcggta
       61 ctggcggcgg cggcacaggt ggcactgatg gcggcggcgc ggcgcccgcg cctggcgccg
      121 ggaccggtac cggcgccgga accggcactg gagctggcgc aggcactggg taacgcggct
      181 tgacccaccg ccggtgcggg tggagggtta aggcgtgtcg agcgagcgca agaagcagct
      241 cgtgcgccgc ctttacgagg aggcctgggg ccggggcgag ctcgccgtga tcgacgagtt
      301 gttcgccgac gagtacacgc ggcacgatct acgcccgatg gccgcgaggc cgggccccgc
      361 gggccagcgc cagatcgcgg aagatttcag gaccgccttt cccgatatcg agatgacggt
      421 cgacctcctc attgcagacg gcgactacgt ggtggcccgc tggaccgccg aggggacgaa
      481 caccggcccg tggggcggcg gcggccaagc gaccggccgg cgtgcccgca tctcaggcgt
      541 gaacatcttc cgcttcgagg gggagcgcgt ggccgagctt tggaaccacc gcgacgacct
      601 cggcatgatg gagcagctcg cggcgccgat ctacagcggc ggcgcctagg ccgcgcggcg
      661 ggcgctagct cgcatcgcgg gcggccgcgg gcgcgagacc gaggtgctcg tggcgtcgca
      721 gaagcgccac ggcaggtcca ccgcccgcgt gatgccgatc cgctcgccca gtacgacctc
      781 cggcgggcgc gagtccgccg gtcgtggaag gaactcgatc ggcccgcgtc cgtcggtgat
      841 cggcgtgccg ttcaggtcga ggccaatccc gagcgcctgg gtgagcttgc ctggccccga
      901 gcagaggtcg tccacgcgat ccatgcgccg tcgcaggcgc atgagatcga cgccgtcgat
      961 cggctcgagc gcgcgaatca gcactgccgc gcccagcccc tcttcctctg ccacggcgtt
     1021 cagtagcgcg tggatcccat aggagcggta cacgtaggca tggcccggcg gcccgaacag
     1081 cacttcagtg cgcggcgtta gcccgttgta ggcgtggcag gccggctcct cctggtggta
     1141 gctctcgacc tccacgatcc ggcccgatgt gtcgccgtgg cggatcgtgc accccacgag
     1201 gtcgcgtgcc accgcgtgga ccgagcgctc gtagaagctt cgccggcgcg gatcgttcac
     1261 gcgctcgcct cttcgagcac gtgacgagcc tgcgcgagct gctccttcac gcggctgaga
     1321 gcggtgccgc cctccgatac cttcgactcg agccaggccc gctcgccgag cagctcgtgg
     1381 aagccgggca cgagctgctc cgagatgccg cgcagctcgt cgtcgctgaa gtccgagagg
     1441 ctccggccgc tctcgagtgc ctcgcgcacg atcccggcca cgatgccgtg ggcttcccgg
     1501 aacggcacgc cgcgagtgac gagcatgtcg gcgatgtcgg tcgccgcgag gaactcgtcc
     1561 gacgcggcct cggcaagccg ctcgcggttg aagcggatgt cgcgcagcac gccggtggcc
     1621 gcggcgaggg cgagctcgag cgtgtcgacc gcgtcgaaca ggtgttcctt gtcctcctgc
     1681 aggtccttgt tgtaggtgag cggcagcccg tgcaacacgc cgtggagccc caccaggtgc
     1741 gccgccacgc gcggcgcctt tgcgcgcagc agctcggccg cgtcggggtt cttcttctgc
     1801 ggcatgatgc tggagccaga cgcgaacgcg tcggagacct cgcagaagcg gaactcctcg
     1861 ctcgaccaga gcacaatctc cgcgcccagc tgcgagaggt gggtcgcgca cgtggccgcc
     1921 gcgctcacgt agtcgatcac gaagtcgcgg ttggagacgg cgtcaatcga gttctcggcg
     1981 atggccggga agccgaggtc ctgcgccacc gacatgcggt cggtggagaa gttcacgccc
     2041 gcgagcgcgc ccgcgccgag cggaagctcg tcggtggtgc tgagcacgaa gccgaagcgg
     2101 tggaagtcgc gccgcagctt ccagaagtag gcgagcaggt ggtgcgatag gtagaccggc
     2161 tgcgcgcgct ggagatgcgt gtagccgggc atcggccagt cgatgtggcg ctcggcgagg
     2221 gtctccagca cacccatcaa gccgaggatc gcgtcgcgcg cccgcagcgc gtgggcgcgt
     2281 acgaacagcg tcgcatcggt cgccacctga tcgttgcgcg agcgcgccgt gtgcatcttg
     2341 ccgccgaccg ggccgatcag ctccgtcacg cggcgctcga tcgccatgtg gacgtcctcg
     2401 tcgcccggct tgacctcgaa gctgtcgtcg tcgatctcgc gttgcaccag ctcgaggccg
     2461 cgctcgatct cagcggcgtc ggcctctggg atcacgccgg agcgcccgag catccgcaca
     2521 tgcgcgcgcg actgctcgat gtcgtagggc gccaagcggt agtcgaacga caccgacgag
     2581 ttgagcgcct ggaagacggg gtcctgcggc tccgagaagc gggacatgcg cgcgcagtct
     2641 acgaggggca aacgcgcagc cgtaggattc cgcgcgatga gagcgctcgt gaccggcgcg
     2701 accggcaaga tcggcaacgc gctcgtgcac ctcctggccg agcggggcca cgaggttcgc
     2761 gcactgctcc gggatccggt gcgcgccgcg ccgcatcttc cggcgggtgc cgagaccgtg
     2821 cagggggacg tgaccgatcc aggttcgctg ccacaggcgg tcgagggctg cgacgtggtg
     2881 ttcaacgcga tgggccttcc ggagcagtgg ctcgccgacg agaacgactt ccaccgggtg
     2941 aacgcgctgg gcagcgagaa cgtgacgaag gcggcgcgag cggccggcgt gccgcggttc
     3001 gtccacacga gcacgatcga cgtgttccac gccgagcgcg gcgcccgctt cgatgagagc
     3061 aacgtggccg actatcccaa gggaactccc tacgagcgct cgaagcagga ggccgagcgg
     3121 ctggtgctgg ccgcggccgg tgacgggctc gaagtggtga tcgcgaaccc gtccgccgtg
     3181 tacgggcccg ggcccggggg agcgggcagc tcgttcgagg agaacctgat gcggccgatc
     3241 gtgaagaagc gcctgccggc cgtgccgccc ggcgggatgg gcgtcacgtt caatcgcggg
     3301 gcggccgacg cgctcctgcg aattgccgag cgtggcagac cgggtgagcg ctacatcgtc
     3361 tgcgaccgcc acgcgacgat gaaggagctc gcggacagcg tggtccgcat cgccggccgc
     3421 ggccgcgtgc cgcccgtgct ccccgtgccg gtcgccaagg cgatggccgg agcgggcgag
     3481 gccctctcac gcgcgatcca ccggcctccg ctcctgcccc gcgggcagct gtacttcttc
     3541 ctgtgggatg ccgcgccgga ctcgagcaag gtgcaggagg agctcggctg ggagccgacg
     3601 ccgctcgacg aagggctccg cgtgacgcta gcggagatgg acccgccgct tgactgaccg
     3661 cctcacgacg acgatccggc ctgcgaggcc cgaggaccgg gacgcggtcg ccgacctcgt
     3721 ctacctgacg gcgggcgacg cgtacgacct gttcgcgggc agcagggagg cggctgtgga
     3781 gctgatccgg gcggccttcg agcgggacgg caccgacgcg agccgcgatg tcgtgaccgt
     3841 cgccgaggtg aacgggcgcg tggccggcgc gatggccgcg ttcccggtgg ccgagggccg
     3901 cgaccgctcg ggccgcttcc tgcgcatggt gctgcgcatg cgcccaccct ggcgctgggg
     3961 cggcgtcctg cggctgtggt gggcagggac gcgcggcggt ccgcggccgc cgttcgattc
     4021 gctctacgtg gatgcgctcg ccacgcaccc ggagttccgg cgccgcggca tcgcgctcgc
     4081 gctactcgac gaggcggagc gcgccgcgcg cgaccgcggg ctagcgcagg tggcgctcga
     4141 cacggcggcg gggaacacgg ccgcgaggtt gctctacgag cgcgccggct tcgtgctcac
     4201 cgaggagcgc cgcgcgcggg ggccgatccc tgcgctagtg ggcttcgcca agcgggtcgc
     4261 ctgagcgcgt gagcgcttgc gccaccacct cgcgcagcgc ggaggccaca cgctcgatct
     4321 cgccctcgcc gagctgcggg aagaacggca acgccagcga acgctcagcc acgtcctcgc
     4381 agacggggaa ctctcccggc cggtggccga agcgctcgcg gtagaagctc atgagatgga
     4441 tggcgggcag gtagggcttc gagtcgatcc cgcgggctcg cagctcctcg atggcgtcgt
     4501 cgcgcgggac gccatgcggc agctgcacca cgtacacgaa ccagctccgc cgatcgccgc
     4561 cgtcgtcggg gcagggaagc tcgagccctt cgatgtcggc gagcgcctcg ccgtatagcg
     4621 ccgccacgcg cgaacgcgcc gcgagcagct cctccaggcg ctcgagctgc gcgatgccga
     4681 gcgcgcacgc gaggtccgag agcctgtagt tgaaccccag gcgatcgtgg tcgagccagc
     4741 ccatgtcggg ggcgcgaccc tgattgcgct ccgagtcgat gcgctccttc gactcggcgt
     4801 ccgggcacac caccatcccg ccctcgccgg tcgtcatctg cttgttcgga tagaacgcga
     4861 acgtcgagag gtgtccgcgg gcgcccaccg ccggaccgtc gccgtggacc gcgccgagcg
     4921 cctcgcaggc gtcctccacg atccagagcc cgcgttcgcg ggcgatcgcc tcgaccgccg
     4981 gcatgtccac cggatagccg aagatgtgga ccggcaggat cccgctcgtc cgctcaccga
     5041 cggcggccgc ggcggccgcc gggtcgatgt tcagcgtgcg cgggtcgatg tcgcggaaca
     5101 ccggcttggc gccctcgtag atcacgcagt tcgacgaggc gacgaagctg aacggcgtgg
     5161 tgatcacctc gtcgccggca tcgatgccgg cgacgcgcac ggcgaggtgg aggccggctg
     5221 tgccgctcga caccgcggac gcgctcggca cgccgagccg ctgcgcgaag gcgccctcga
     5281 actcgccgag gcgcgggccg agagacaggc gtcccgaccg aagcgtgtcg agcacgagcc
     5341 gctcctcgcg ctcgccgatc agcggcttcg cgagcg
//
LOCUS       DASVXS010000155         8180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_233475, whole genome shotgun sequence.
ACCESSION   DASVXS010000155 DASVXS010000000
VERSION     DASVXS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233475"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VF752_08565"
     CDS             complement(<1..1236)
                     /locus_tag="VF752_08565"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8855641.1"
                     /translation="MATKRKQYDVADLGLAGVGESRIAWADAQMPVLRQIRARFEDER
                     PLEGVPVACCLHVTAETANLARTLAAGGAAVALCASNPLSTQDDVAAALVDRHGTAVF
                     AIQGEDAETYYAHIDAVCDRHPRVTMDDGADLISVLHDTRSEQLGEIGGGTENTTTGV
                     IRVRALEAQGKLAFPVLAVDDAATKHLVDNRYGTGQSTLDGILRATNALIAGRRVVVI
                     GYGACGQGVAERAKGAGARVIVCEVNPLLALEAALDGYDVMPAVEAAAAGDLFVTVTG
                     NRDVLTKEHFDVMKDGAILANAGHFDVEIDKTSLDRLTKERRPVRPHVEEHVLKDGRR
                     LFLLADGRVVNLAAAEGNPASVMDISFANQALAAEHIVLHVAELEPRVHDVPPSVDEE
                     IARLKLEALGLSIDELTEAQ"
     gene            complement(1267..2718)
                     /gene="ahcY"
                     /locus_tag="VF752_08570"
     CDS             complement(1267..2718)
                     /gene="ahcY"
                     /locus_tag="VF752_08570"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8855642.1"
                     /translation="MTTTQTHQDFKVADLSLAEFGRKEIRLAEHEMPGLMKTREEFAA
                     SQPLKGGKIMGSLHMTVQTAVLIETLVALGADVRWVSCNIFSTQDHAAAAVAVGPNGT
                     PDDPRGIPVFAWKGETLDEYWWCTEQALTWPGDDGPNMILDDGGDATLLVHKGTEYEK
                     AGAVPDPESAESEEFQVILRLLQRTLAEDPQKWTRIGERIQGVTEETTTGVHRLYQLA
                     EQGELLFPTINVNDSVTKSKFDNLYGCRHSLIDGINRATDVMIGGKVALVCGYGDVGK
                     GCAASLRGQGARVLISEIDPICALQAAMEGYEIVRLDDVVDKADIFVTATGNKDIITA
                     EHMGRMKHQAIVGNIGHFDNEIDMAGLQKVPDIKRVNIKPQVDEWVFPDGHSIIVLSE
                     GRLLNLGNATGHPSFVMSNSFTNQTIAQIELFTKPGEYEKKVYVLPKHLDEKVARLHL
                     DALGVSLTKLTPEQAAYIGVPVDGPYKPDHYRY"
     gene            complement(2748..3110)
                     /locus_tag="VF752_08575"
     CDS             complement(2748..3110)
                     /locus_tag="VF752_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8855643.1"
                     /translation="MADGLTIHEAAETTGWSPRMLRYLERVGLVNPPRTGAGYRVFGP
                     GELQRLRTLRELLARFDCGLSDVAFAKRVKDDDELREAVDRWLEAEARRPEGVATDDW
                     LRWEQEKHQRLLAHADAA"
     gene            complement(3146..4180)
                     /locus_tag="VF752_08580"
     CDS             complement(3146..4180)
                     /locus_tag="VF752_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX8855644.1"
                     /translation="MTELTRDAIRSVDPQDMLGVVLEQPAHLTDALWRVESANAPKTD
                     SPGGLIVCGMGGSAIGADLAAAAIGERAERPIRTVRGYSIGPGVGEHTLVLCSSYSGN
                     TEETLACYEQAREAGAPRIALTTGGRLAELAREDGIPVIGVPGGIQPRAAVAYMTVGS
                     LECAALAGAAPSLTAEVEAASRLLGQLVDEWGPDSGDDSLAKSLARTLQRTTPVVYGA
                     GATGPVARRWKTQVNENAKLPAFWGELPEVDHNEICGFESAESFGAVFLDDPSAPERL
                     RRRIELTAESARSGSAAVELVEGRGESALERVMSLVLLGDLVSVYLAALDDTDPTPVA
                     PIEDFKRKLA"
     gene            complement(4177..5205)
                     /locus_tag="VF752_08585"
     CDS             complement(4177..5205)
                     /locus_tag="VF752_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX8855645.1"
                     /translation="MQALVLAGGEGTRLRPLTLTVPKPVMPLAGRPFLSFMLDWLREH
                     GVDDVLLSCGFLAHAVEEVLGEEYAGTRLRYVHEDEPLGTAGPVRLAFDEGLLEDRLF
                     VLNGDVLTDMDLTNQLAQHERTGALATLALIDVEDTSSYGVVPTGEDGGVLDFLEKQD
                     GPAPTNRINAGAYVLERALVERIPDGRAVSFEQEIFPALVGDGLYGYQAAGYWIDIGT
                     PERYLEATYDLLAGTVHSTLPPRDETGSLVYEPAVTAGAHIGPQSVVGPRGSIGADAA
                     VERSVLFPNVTVGEGARIVESVVAEGSRVGANAELGPDAIVGAEAVIAPGAIVPPGTK
                     VEPQEHVE"
     gene            complement(5213..6490)
                     /locus_tag="VF752_08590"
     CDS             complement(5213..6490)
                     /locus_tag="VF752_08590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX8855646.1"
                     /translation="MAEKEPIGVIGTGYVGLVQGTCLAELGHEVWCRDIVQEKVDALN
                     RGEVPIYEPGLAELIEKNRERLHFTTDMREVVTNARLLFCCVDTPHTYSGDADLSRVE
                     QVVEEIGDLGEHALVMKSTVPVGTGRAITRGREELAYVSNPEFLKEGSAVGDFMRPDR
                     VVVGADEASEEYRERVAALYEPLGAPIIRTDVASAEMVKLASNAFLATKISFINEIAN
                     VSEELGADVTEVARGMGLDSRIGPKFLQAGIGWGGSCFPKDVQALKQLAGNTGYHFQL
                     LTAVLEVNELQKRRTIGKLQKHLGSLVGKNIALLGVAFKPNTDDIREATSLVLAGRLR
                     GEGANVRVYDPIAAERAQDILRDAEICDSAEKALEGADAAVLVTEWPEFRELDWANDV
                     KHSMKQPIVIDGRNFLDRDALEAAGFTYEGVGR"
     gene            complement(6503..7930)
                     /locus_tag="VF752_08595"
     CDS             complement(6503..7930)
                     /locus_tag="VF752_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX8855647.1"
                     /translation="MPRPGRGLWKRLVIGGLLIVAACAAATSVAAFHEVQRVVNAFNQ
                     NKSINLGPQILAKTDPGKPQTIMLLGTDARSKTARDYSGTARSDTIILVRLDPSKNAT
                     AMLSLPRDLKVQIPGHGVSKINDAYALGGIKLTVETVKQLTGLRINHVISVDFRGFAT
                     AVDALGCVWVDIDRRYYNQNQAYATIDVQPGYQKLCGSPALDYVRFRHLDNDIVRAAR
                     QQQFLSAIKQQVGFGELIGQRAKLLKIFGKYTRSDIRGRTQVLRILKLAIASAGHPVQ
                     EVHFEGNIGPSYVTASANTVQKLASQFLGVQTVKQATHKAKKTRKRRHRRKDPASLGL
                     VDVSGNTRQMALNAIGAGLKMRVLYPKLATRSSQAVNPARTYSFRAPDGRVYRAYRMV
                     FGGLGLGQYWGVQGMTWKNPPLLQGDHDTRTIGGRKFQIYYEGDKIDMVAWITRDGLY
                     YVKNTLSGSLDNDQMLAIARSCRSL"
     gene            complement(7923..>8180)
                     /locus_tag="VF752_08600"
     CDS             complement(7923..>8180)
                     /locus_tag="VF752_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855648.1"
                     /translation="MLAQAISSEATAEKISVGRDGASDAISFLDAGVPAVEFGPVGAG
                     HHGPEEWVSISSLTRYRETLVRFVQSIPAAGKKEHLRVAGA"
BASE COUNT         1180 a         2821 c         2779 g         1400 t
ORIGIN      
        1 ctgcgcctcg gtgagctcgt cgatcgagag accgagcgcc tcgagtttca gccgcgcgat
       61 ctcctcgtcc acgctcggcg gcacgtcgtg tacgcgcggc tcgagctcgg caacgtgaag
      121 cacgatgtgc tcggcggcga gcgcctggtt cgcgaagctg atgtccatca cggatgccgg
      181 gttgccctcg gcggccgcga ggttcaccac gcgcccatcg gcgagcagaa acagtcgccg
      241 cccgtccttc agcacgtgct cctccacgtg cgggcgcacc ggccggcgct ccttcgtgag
      301 gcggtcgagc gatgtcttgt cgatctccac gtcgaagtgc ccggcgttcg ccaggatcgc
      361 cccgtccttc atcacgtcga agtgctcctt cgtgagcacg tcccgattgc cggtgaccgt
      421 cacaaagagg tcgcctgccg cggcggcctc gaccgcgggc atcacgtcgt agccgtcgag
      481 cgcagcctcc agcgcgagca gcgggttcac ctcgcacacg attacgcgcg cgcccgctcc
      541 cttcgcgcgc tcggccacgc cttggccgca ggccccgtag ccgatcacga ccactcgcct
      601 cccggcaatc aacgcgttcg tcgcgcggag gatcccgtcg agcgtcgact ggcccgtgcc
      661 gtagcggttg tccacgaggt gcttggtggc ggcgtcgtcg accgcgagca ccggaaacgc
      721 gagctttccc tgcgcctcga gcgcgcgaac gcgtatcacg ccggtcgtgg tgttctcggt
      781 gccgccaccg atctcaccga gctgctccga gcgtgtgtcg tgcaggaccg agatgaggtc
      841 ggcgccgtcg tccatcgtca cgcgcgggtg gcggtcgcac accgcgtcga tgtgcgcgta
      901 gtaggtctcc gcgtcctcgc cctggatcgc gaacacggcc gtgccgtggc gatcgacgag
      961 cgcggccgcg acgtcgtcct gggtggagag aggattcgat gcgcagagcg ccacggcggc
     1021 gccgcccgcg gcgagtgtgc gcgcgaggtt cgccgtctcg gcggtcacgt gcaggcagca
     1081 agcgaccggg actccctcga gcggccgctc gtcctcgaag cgagcgcgga tctggcgcag
     1141 caccggcatc tgcgcatctg cccaggcgat gcgcgactcg ccgaccccgg cgaggccgag
     1201 gtcggcgacg tcgtattgct tgcgcttggt ggccatgacc gccgcctcgc gcgcctgggt
     1261 gcgcgcctag tagcggtagt ggtccggctt gtacgggccg tcgaccggca cgccgatgta
     1321 ggcggcctgc tcgggcgtca gcttcgtgag cgagacgccg agcgcgtcga ggtgcagccg
     1381 cgcgaccttc tcatcgaggt gcttcggaag cacatagacc ttcttctcgt attcgcccgg
     1441 cttcgtgaac agctcgatct gggcgatcgt ctggttcgtg aacgagttcg acatcacgaa
     1501 gctcgggtgg ccggtggcgt tgcccaggtt caacaggcgg ccctccgaca gcacgatgat
     1561 cgagtggccg tcgggaaaca cccactcgtc cacctgcggc ttgatgttga cccgcttgat
     1621 gtccggcacc ttctgaaggc cggccatgtc gatctcgttg tcgaagtggc cgatgttgcc
     1681 cacgatcgcc tgatgcttca ttcggcccat gtgctcggcc gtgatgatgt ccttgttgcc
     1741 ggtggcggtg acgaagatgt ccgccttgtc gaccacgtcg tcgaggcgaa cgatctcgta
     1801 gccctccatt gccgcctgca gcgcgcagat cgggtcgatc tcgctgatga gcacgcgcgc
     1861 tccctggcct cgcagtgagg cggcgcagcc cttgccgacg tcgccgtagc cgcacacgag
     1921 cgcgaccttg ccgccgatca tcacgtcggt ggcgcggttg atgccgtcga ttagcgaatg
     1981 gcggcagccg tagaggttgt cgaacttgct cttcgtgacc gagtcgttca cgttgattgt
     2041 cgggaacagc agctcgccct gctcggcgag ctggtagagc cggtggacgc cggtggtggt
     2101 ctcctcggtg acgccctgga tgcgttcgcc gatgcgcgtc cacttctgcg gatcctccgc
     2161 aagcgtgcgc tggagcagcc gcaggatcac ctgaaactcc tccgactcgg cgctttcggg
     2221 gtccggcacg gcgccggcct tctcgtactc ggtgcccttg tgcacgagca gcgtggcgtc
     2281 gccaccgtcg tcgaggatca tgttcgggcc gtcgtcgccg ggccaggtga gcgcctgctc
     2341 ggtgcaccac cagtactcgt cgagcgtctc gcccttccag gcgaacaccg ggatgccccg
     2401 cgggtcgtcc ggcgtgccat tcggacccac cgccaccgcg gccgccgcgt ggtcctgcgt
     2461 cgagaagatg ttgcagctca cccagcgcac gtcggcgccg agcgcaacga gcgtctcgat
     2521 caggaccgcc gtctgcacgg tcatgtggag cgagcccatg atcttcccgc ccttcagcgg
     2581 ctgcgacgcc gcgaactcct cgcgcgtctt catcaagccg ggcatctcgt gctcggcgag
     2641 cctgatctcc ttgcggccga actcggcgag ggagaggtcg gcgaccttga agtcttgatg
     2701 ggtctgggtg gtcgtcatgc ggtctccagt cggggtcggg tcgggcgcta cgcggcgtcg
     2761 gcgtgcgcga gcaggcgctg gtgcttctcc tgctcccagc gcagccaatc gtcggtggcg
     2821 acgccctccg ggcgccgcgc ctcggcttcg agccagcggt cgaccgcttc gcggagttca
     2881 tcgtcgtcct tcacccgctt ggcgaaggcg acgtcggaga gtccacagtc gaaccgggcc
     2941 agcagctcac ggagcgttcg cagccgctgc agctcgcccg gtccgaaaac gcggtagccc
     3001 gcgccggttc tcggcgggtt gacgagcccc acgcgctcga gatagcgcaa cattcgaggc
     3061 gaccaaccgg tcgtctccgc tgcctcgtgg atggtgagcc cgtctgccat tacgggagcc
     3121 aaccttacca cgattgtgtc aaggttcagg cgagcttgcg cttgaagtcc tcgatcggcg
     3181 cgaccggcgt ggggtcggtg tcgtcgagcg cggcgagata gaccgatacg agatcgccga
     3241 gcagcacgag cgacatcacc cgctcgagcg cgctctcgcc gcggccctcg acgagctcga
     3301 ccgcggctga gccggagcgc gcgctctccg ccgtcagctc gatgcgccgt cgcaggcgct
     3361 cgggcgccga cgggtcgtcg aggaagacgg ccccgaagct ctccgcgctc tcgaaaccgc
     3421 agatctcgtt gtggtcgacc tcgggaagct cgccccagaa ggccggcagc ttggcgttct
     3481 cgttcacctg cgtcttccag cggcgggcga cggggccggt ggctcccgcg ccgtagacga
     3541 ctggcgtcgt tctttggagt gtccgcgcga gcgacttcgc gagcgagtcg tccccggaat
     3601 cggggcccca ttcatctacg agctggccga gcagtcgcga cgccgcctcg acctcggcgg
     3661 tgagcgatgg agccgcaccg gcgagtgccg cgcactccag cgagccgacg gtcatgtagg
     3721 cgacggcggc gcgcggctgg atgccaccgg gcacgccgat cacggggatg ccgtcctcgc
     3781 gcgccagctc cgccagcctg ccgccggtgg tgagcgcaat acgaggcgca cccgcctctc
     3841 gcgcctgctc gtagcaggcg agcgtctcct cggtgttgcc cgagtagctc gagcagagca
     3901 cgagggtgtg ctctccgacg ccggggccga tcgagtagcc gcgtaccgtg cggatcggcc
     3961 gctcggcgcg ctccccgatc gcggcggcgg ccaggtcggc gccgatcgcg gagccaccca
     4021 tgccgcagac gatcaagcca ccgggcgagt cggtcttcgg cgcgttcgcc gactcgacgc
     4081 gccacagcgc gtcggtgaga tgcgccggct gctcgagcac gacgcccagc atgtcctgtg
     4141 gatctacgga gcggatcgcg tcgcgcgtca actcggtcat tcgacgtgct cctgcggctc
     4201 gaccttcgtc ccgggcggca cgatggcgcc aggtgcgata accgcctccg cgcccacgat
     4261 cgcatcggga ccgagctcag cgttcgcgcc gacgcgcgag ccctcggcca ccacgctctc
     4321 gacgattcgc gcgccctcgc ccaccgtcac gttggggaag agcacggagc gctcgacggc
     4381 cgcgtcggcg ccgatgctcc cgcgaggccc gaccaccgac tgcgggccga tatgcgcgcc
     4441 ggccgtaacg gccggctcgt agacgagcga tccggtctcg tcgcgtgggg gcagcgtgct
     4501 gtgcacggtc cctgcgagga ggtcgtacgt agcctcgaga tagcgctcgg gggtgccgat
     4561 gtcgatccag taaccggcgg cttggtatcc gtagaggccg tcgcccacga gcgccgggaa
     4621 gatctcctgc tcgaacgaca cggcgcgccc gtccggtatg cgctcaacaa gcgcgcgctc
     4681 gagcacgtac gcgccggcgt tgatccggtt tgtcggagcc ggcccgtcct gcttctcgag
     4741 gaagtcgaga acgcccccat cttcgccggt gggcacgacc ccgtagctcg acgtgtcctc
     4801 gacgtcgatc agtgccagcg tggcgagcgc gccggtgcgt tcgtgttgtg cgagctggtt
     4861 ggtgaggtcc atgtcggtga ggacgtcgcc gttgagtacg aacagccgat cctcgagcag
     4921 accctcgtcg aacgcgagcc gcaccgggcc ggccgtgccg agcggctcgt cctcgtgcac
     4981 gtagcggagg cgcgtgccgg cgtactcctc gcccagcacc tcttccacgg catgtgcgag
     5041 gaagccgcac gacagcagca cgtcgtcgac tccgtgttcg cggagccagt cgagcatgaa
     5101 gctgaggaac gggcggcccg caagtggcat gactggcttc ggaaccgtga gcgtcagagg
     5161 cctgagccgc gtcccctctc ccccggcgag gacgagcgcc tgcacgcgag ccctacctgc
     5221 cgacgccctc gtaggtgaaa ccggccgcct cgagcgcgtc gcggtcgagg aagttgcggc
     5281 cgtcgatgac gatcggctgc ttcatggagt gcttgacgtc gttggcccag tcgagctcgc
     5341 ggaactccgg ccactccgtc acgagcacgg cggcgtccgc gccctcgagc gccttctccg
     5401 cggagtcgca gatctcagca tcgcgcagga tgtcctgcgc acgctccgcg gcgatcgggt
     5461 cgtagacccg cacgttcgct ccctcaccgc gcagacgccc ggcgagcacc aggctcgtcg
     5521 cctcgcggat gtcgtcggtg ttcggcttga acgcgacacc gagcagcgcg atgttcttac
     5581 cgacgagcga cccgaggtgc ttttgcagct tgccgatcgt gcggcgcttc tgcagctcgt
     5641 tgacctcgag cacggcggtc agtagctgga agtggtagcc cgtattgccg gcgagctgct
     5701 tcagggcctg aacatctttt gggaaacagg agccgcccca cccgatccct gcctgcagga
     5761 acttcgggcc gatgcgcgag tcgaggccca tgccgcgcgc cacctcggtc acgtcggcac
     5821 cgagctcctc cgacacgttc gcgatctcgt tgataaatga gatcttcgtg gcgaggaagg
     5881 cgttcgaggc gagcttgacc atctcggcgc tcgccacgtc ggtgcggatg atcggcgcgc
     5941 cgagcggctc gtagagcgcc gccactcgct cgcggtactc ctccgaagcc tcgtcggcgc
     6001 cgactacgac gcggtccggg cgcatgaagt cgccgaccgc cgagccctcc ttgaggaact
     6061 cggggttcga cacgtacgcg agctcctcgc gcccgcgtgt gatcgcccgc ccggtcccga
     6121 ctggcaccgt cgacttcatc acgagcgcgt gctcgccgag atcgccgatc tcctcgacaa
     6181 cctgctcgac gcgcgagagg tcggcatccc cggagtaggt gtgcggcgtg tcgacgcagc
     6241 agaagagcag gcgcgcgttg gtaacgacct ccctcatgtc ggtcgtgaag tggagccgct
     6301 cgcggttctt ctcgatcagc tcagcgaggc ccggctcgta gatcggcact tcgcctcggt
     6361 tcagcgcgtc gaccttctcc tggacgatgt cgcgacacca gacctcgtgc ccgagctccg
     6421 cgaggcaggt cccctgcacg agcccgacgt agcccgtgcc gattactccg atcggctcct
     6481 tctcagccat gggcggggca gactacaagg accggcagga acgggcgatg gcgagcatct
     6541 ggtcgttgtc gaggctgccc gagagcgtgt tcttcacgta atagaggccg tcgcgggtga
     6601 tccaggcgac catgtcgatc ttgtcgccct cgtagtagat ctggaacttg cggccgccga
     6661 tcgtacgggt gtcgtggtcg ccctggagca gcggcgggtt cttccaggtc atgccctgaa
     6721 cgccccagta ctggccgagg ccaaggccgc cgaacaccat gcggtaggcg cggtagacgc
     6781 gcccgtcggg cgcccggaac gagtacgtac gcgccgggtt cacagcctgg ctcgagcgcg
     6841 tcgcgagctt gggatagagc acgcgcatct tgaggcccgc accgatcgcg ttgagcgcca
     6901 tctggcgcgt attccccgac acgtcgacaa gtcccagcga ggccgggtcc ttgcggcgat
     6961 ggcggcgctt gcgcgtcttc ttcgccttgt gggtggcctg cttcaccgtc tgcacgccga
     7021 ggaactgcga cgccagcttc tgcaccgtgt tggcgctcgc cgtcacatag ctcgggccga
     7081 tgttgccctc gaagtgcacc tcctgcactg ggtgaccagc cgaggcgatc gcgagcttga
     7141 ggatgcgcag cacctgggtc ctgccgcgga tgtccgagcg cgtgtacttg ccgaagatct
     7201 tcagcagctt ggcgcgctgc ccgatcagct caccgaagcc gacctgctgc ttgatcgcgg
     7261 acaggaactg ctgctggcgg gccgcgcgga cgatgtcgtt gtcgaggtgg cggaagcgca
     7321 cgtaatcgag cgccggcgag ccgcagagct tctggtagcc cggctggacg tcgatcgtcg
     7381 cgtaggcctg gttctggttg tagtagcggc ggtcgatgtc gacccagacg cagccgagcg
     7441 cgtcgaccgc cgtggcgaag ccgcggaagt caacgctgat cacgtggttg atgcgcaggc
     7501 cggtgagctg cttgacggtc tcgacggtga gcttgatccc gccgagcgcg taggcgtcat
     7561 tgatcttcga gaccccgtgg ccgggaatct gcaccttgag gtcgcgcggc agcgacaaca
     7621 tcgccgtcgc gttcttcgac gggtcgagcc ggacgaggat gatcgtgtcg gagcgcgccg
     7681 tgccgctgta gtcgcgcgcc gtcttcgagc gtgcgtcggt gccgagcagc atgatcgtct
     7741 gcggcttgcc cggatctgtc ttcgccagga tctgcggccc caggttgatc gacttgttct
     7801 ggttgaacgc gttgaccacc cgctgcacct cgtggaaggc cgcaaccgac gttgccgcgg
     7861 cgcaagcggc cacgatcagc agcccgccga tcacgagccg cttccagagg ccgcgtcccg
     7921 gcctaggcac cggcgacacg caggtgctcc ttcttgcccg cggcggggat cgactgcacg
     7981 aaccgcacga gcgtctcgcg gtaacgggtg agcgacgaaa tcgagaccca ctcctcgggc
     8041 ccgtggtggc cggcgccgac cggcccgaac tcgaccgccg gcacgccggc atcgaggaag
     8101 ctgatcgcgt cggaggcgcc gtcgcggccc accgagatct tctccgcggt ggcctcgctc
     8161 gagattgcct gcgccagcat
//
LOCUS       DASVXS010000156        15058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_728065, whole genome shotgun sequence.
ACCESSION   DASVXS010000156 DASVXS010000000
VERSION     DASVXS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15058
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728065"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..538
                     /locus_tag="VF752_08605"
     CDS             284..538
                     /locus_tag="VF752_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855649.1"
                     /translation="MLSPNRLIPFPNRLARIARAHEGPDDTPTMELWDAWVFAEVDSK
                     LALRSWWDAPSGSKSVAFATYLAALDREEQAAAVLAARFA"
     gene            859..1647
                     /locus_tag="VF752_08610"
     CDS             859..1647
                     /locus_tag="VF752_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8855650.1"
                     /translation="MPSNATAPADGHVTWDPRAQERTTRLFRRLRRFGDTAARDQLIE
                     LYMPLARRLALRHTGSREPFDDMMQVAVVGLILAVDRFDPERGIPFAGFAKPTIAGEL
                     KRHLRDHAWCLHVPRELQDRALRVVGAADRLSGRGSVSESLEELAAHCKITVDEAAEA
                     IAAWHAYTPDSLDVPDPESPSRLRVESSGARDPGYVKVEHLSVISQALAALSPTEQTI
                     LALRFGADLSQTEIAERVGISQMHVSRLLGRALARLEVVVRANG"
     gene            complement(2011..2442)
                     /locus_tag="VF752_08615"
     CDS             complement(2011..2442)
                     /locus_tag="VF752_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8855651.1"
                     /translation="MKRSEALIPLSHDHQHGLDAALRLRRASADSASEAAAHFEQFWT
                     GEGRHHFEIEERVLLGALPDSTPGWSDLTGRVRREHEDIRERAAAVADGGRDVSALRQ
                     LGDRLHDHIRFEERELFLILEASLSDAELSRLGAAIAQAHG"
     gene            complement(2453..3373)
                     /locus_tag="VF752_08620"
     CDS             complement(2453..3373)
                     /locus_tag="VF752_08620"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8855652.1"
                     /translation="MCDHVDPGTREGFILVVETPPTSITRQRNGSDGEIRRDELADFL
                     RTRREAIQPDEVGLPNGGRRRTPGLRREEVALLAGVGTTWYTWLEQGRDVRASLDVLE
                     SIARALRLTPAERNHLILLGRGEQAPSLCSPAEKVSPAVRRLIENLGPNPAHVIGRRW
                     DLLAWNRAYSAVFGDPAETPKSFRNTLWLAFMDPARRELITDWNKGARLMVARFRADS
                     AKHIGDPAFEELIAALNDSSPEFRKWWKRHEVAGSGEGRKTLNHPLAGTLVFEHAVFR
                     PAEQPEQRVAMFSPLPDEETPAKLAALLEE"
     gene            3381..4874
                     /locus_tag="VF752_08625"
     CDS             3381..4874
                     /locus_tag="VF752_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855653.1"
                     /translation="MPAIAKLPLRRQATSDRRDLNLLLVVLCLAQFMVVLDVSVVNVA
                     LPSIRRGLGFSATDLEWVINAYTLTFAGFLMLGGRAADLLGRRRMFLAGIALFTLASL
                     ACAASDSRALLLGARAVQGVGAAVVSPASLSIIATSFAAGSERNRALGVWGAMAGLGA
                     ASGVLLGGVLTQTLGWWAIFAVNVPVGAAVVALAPAVVPEGRRAGATRNFDLSGALLV
                     TAGLTALTYGIVRSDALGWTSAGVLTPIATGVGLLLVFLFVEGRLASAPLVPLSIFRL
                     PLLRAANLVVLLMYSGTLAMFFFVSLYLQQVLHFDAIETGLAFLPMTLFATTGSTLAP
                     RLVARFGARRVVTAGMLCVATGLALLTGIAPGASYALHVLPGGVLTTLGLGLTLVPST
                     IMAVQGVAPEESGVASGLLNTSRFVGGALGIAVLGTIAASHTRHVAEAGTGALRALSD
                     GYQLGLGIGAIVCVIGAVIAATFLRERPQPEPATVPAEGEEAEALAA"
     gene            4982..5542
                     /locus_tag="VF752_08630"
     CDS             4982..5542
                     /locus_tag="VF752_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855654.1"
                     /translation="MSHRRIFGGVLGLAVAIAGTGVAFAAHPAAAPKNVTVKEKFSMT
                     MKPNRFIQDGMRFDKDVYTVKSGGKLTFVLNQAQEGPHTLSIVRPKDLPKTAQQVNKC
                     KICEKLGKAHGADPNSDAPPKFPFLENGVGQDTPPKLDKPGDSGLVGPNRGAKVSFAV
                     TAKPGSTLTFMCLIHPWMQAKLKVVK"
     gene            5550..6440
                     /locus_tag="VF752_08635"
     CDS             5550..6440
                     /locus_tag="VF752_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX8855655.1"
                     /translation="MKLSKLARALAAAGALAALIPGAAQARVLDYWVAAVPTQWNIVP
                     NGRDAIMGMPVDPADTIFPTVVYRRYSPHWRRQLANAERSAADGLAIPGPLIRARVGD
                     RLRIHFKNMDTLRHDPHSMHFHGVHYKPSSDGAYVPGFSGPDADVKPGRLYTYRLTAG
                     RDSAGVWPYHDHSPSMEQSIGGGLFGMLSILGRHERRADREFEVVFTPFGKFMSIDGR
                     AFVGNTPVFKSKVGDLVQWDVMAMAPSTTHSMFTATAGSARTACRGTRRPSVRPRASD
                     SAGSRRTPERGSTTATSRIT"
     gene            6478..7386
                     /locus_tag="VF752_08640"
     CDS             6478..7386
                     /locus_tag="VF752_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8855656.1"
                     /translation="MRSRALAAALCAVGTLAVAAPASAATHEVHALFAEFSPTPLDVL
                     PGETVEWMNMSERRHTVTADDGSFESGDQFNPGASFSQTFDSVGTYLYHCRLHAGMVG
                     EIDVRRVTLDPLPTAPVPQGDALALSGRTATPVQPVTIELDSGHGFAPVGTATPSADG
                     SWKATVKAQQTGDYRAVVGADTSESRRLVVSDRKITIRVTRTGVAVSVRPALPYARVV
                     LQQYLRERFGWWPQKRGRLDYVSTASFRVSRPARVRVALLGSDDWTPLVTSKVVTLKR
                     RASGSRASRSRRSADPRATAAAPRAR"
     gene            complement(7310..7666)
                     /locus_tag="VF752_08645"
     CDS             complement(7310..7666)
                     /locus_tag="VF752_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855657.1"
                     /translation="MDRPTATVSELVRQAVEVTDPAGDDESLSHFEEQLEDDDEPITA
                     VENLEERLALAAEGADYESDDPAVSVATAVVLYLAHERGRANYDRDPDELIELAVRRQ
                     WHGDPPSDVSDWLESR"
     gene            7692..8597
                     /locus_tag="VF752_08650"
     CDS             7692..8597
                     /locus_tag="VF752_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8855658.1"
                     /translation="MTARGFALFAAVSLVWGLPYLFIKIAVNGGFSPSGVAFVRVALA
                     AAVLVPIAVWRGGVRELRGRWPAVMAFALLEVCVPFPLIAFGEQHVASSLAAILVATL
                     PLVIAVLAIRLDPEERATGVRLAGLIVGLVGVALLLGVDVAGDGKELLGAFAILVATV
                     GYALGAMIVKRSLSDLDPLAPIAGSLLVASVVLAPAAALDWPSRSPSAGTLGALAVLG
                     LVCTALAFLLYFALIAEAGPSKASVITYLNPVVALALGVIFLDESVSAATAAGLALIL
                     AGSWVATGGRRETPSAVAVEPKGVA"
     gene            8594..8992
                     /gene="tsaA"
                     /locus_tag="VF752_08655"
     CDS             8594..8992
                     /gene="tsaA"
                     /locus_tag="VF752_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX8855659.1"
                     /translation="MSNETFDAVQIGTVHSPLTDTASAPKQGREGAPEAAIEFSADVL
                     DALESLRVGDRVIVVTWLDRARRDVLRVHPRDNPANPLRGVFNTRSADRPNPIGLHEV
                     EIVAIDGNTVRVRDLEAIDGTPVLDLKPVI"
     gene            9055..10272
                     /locus_tag="VF752_08660"
     CDS             9055..10272
                     /locus_tag="VF752_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8855660.1"
                     /translation="MDWTLAFVALAVLIVAAVSGRLSDGPVTPAMVFVGIGVLIGPRV
                     LDEVDLSPLSAPVRTLAEAALTVVLFSDASRIDMRALRREYGLPVRLLAIGLPLTIAA
                     GTAAGIAVFGGLTALEALVLAVVLAPTDAALGQAVVTEPRLPLRVRQGLNVESGLNDG
                     ICVPLLLIALAGADVNSHIDSGHDALKVVFEEIGYGLLGGVSAGVAAAAIVVIGRRRG
                     LIADSWLPIVPVAAAALAYGVATPLEGSGFIAAFVAGMCFGALLRREPQPMEAFGEEI
                     GQLLSGVTFVAFGAVLLGPALGDLSWRIGCYALLSLTVVRMLPVALSLMGSGARRQTV
                     AFLGWFGPRGLASIVFAVIVLEESKLPHLNTILLTTYATVGLSVLLHGLSAAPLARAY
                     AGWHQRHPRKDDL"
     gene            complement(11042..11245)
                     /locus_tag="VF752_08665"
     CDS             complement(11042..11245)
                     /locus_tag="VF752_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855661.1"
                     /translation="MASGRRNVDDESGRARDDALVAQQRSRRLVGLRIREAAMVNVQL
                     VAAPDPYGEQVARRTDSRGTRAR"
     gene            11258..11962
                     /locus_tag="VF752_08670"
     CDS             11258..11962
                     /locus_tag="VF752_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8855662.1"
                     /translation="MAASPDGAFFPHERDLLDVYARYAAAVLDTATALDEARRRHEEA
                     QALLGLAQSVATANTSQDVAERLAGAVPAVVDCDRVFVFLWDEVEQALVHHASGGDPG
                     PSGDIARQLRITPDDTPRLRELRASPMPEPIFFDAHPDDPFLEATMRATGALALVVVP
                     IVANERFYGTMNVSVTERPERLAPSPALLDTLAGAVAQAATALDNARLIESMAHQARQ
                     PTRPPCLPRGDRRRTL"
     gene            11943..12359
                     /locus_tag="VF752_08675"
     CDS             11943..12359
                     /locus_tag="VF752_08675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8855663.1"
                     /translation="MADGLSERDPEAFTLAMIDIDDFKQINDTHGHPVGDEALRLVAE
                     ALHRAVRDDDRVFRVGGEEFAVLLPGLTAADATAAAERLREAVARIEFLVPLTVSVGL
                     ASWPEGASDRDSLIEHADCALYQAKRAGKDRVSVAA"
     gene            12581..>15058
                     /locus_tag="VF752_08680"
     CDS             12581..>15058
                     /locus_tag="VF752_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8855664.1"
                     /translation="MTTRVTSARVVGRERELAELEAALADAAAGRPSIAFVAGESGIG
                     KTRLLREFQRRAQEADPAARVICGDSVELGEGELPYAPLVAALRPLARARDPVLESLP
                     PAARDELAALLPGLAPAASEEPVGERDDAAQARLFEALLQLFDELGREQPLVVVIEDI
                     HWADRSTRAFLAFMARALANERVLLVSTYRTDELHRRHPLRPLLAELERDATARRIEL
                     QALDREELRELLGDILGAEPGADLVERLWSRSEGNPLFTEELLAASLDGRGGLPPTLR
                     DALMVRIERLGEDTQEVLRVLAAGRRLDHGLLSDASGLDPARLRTALREAVASHIVAA
                     DPEGFHTFRHALLREVVHDDLLPGEHSDLHLALARALERRMELQGTSTYLTAGIAHHY
                     FVAGEQKAALSASVNAARAAEHVYAYGEAAALLERALELWERVPEPERLAGCDRVELL
                     KRAAVAHQGHGDYARQEHLLRKALDLLDEQAEPRGSARLLELMARAQWSLNRSADAMA
                     SAEHALELVQDGEPGEERARLVSWLAKARMLQGRYRESLDVASEAITEAEAAGDTLAE
                     GRARNARGFSLVGLGRVDDGIAELRRAVELAHNRENVSELGSAYGNLADALNQAGRTR
                     EALAVVERGREVVSGFSRPRDWLLVMIAELKWQLGEWDEAEASMPPSERRYVATTTMY
                     VLLQRATFALGRGDHAVARRYLEEIEEFVAESTEPQYLGTWGALRASLSLREGDIDAA
                     RAAVDEALDRIEFCSEDANRIVRLSAIGVAIEAAAIERERDLGEDTALAQFRAEAMVS
                     RLRAGAEGDRPVERAWLAMAEAEGTRVD"
BASE COUNT         2137 a         5245 c         5298 g         2378 t
ORIGIN      
        1 cggcggggaa acagagcccg gattggatgc gcgacgcgcc gaccgccggg ctgggcgctg
       61 gatgagccgt gctctcggcg acccatgtgc gcgcagcggg ggccacgacc agtgaaccgc
      121 gaggcaccgt cccagcaaga ggcttcggcg ctcgtatgag agccgtgcgc gatcacgcgg
      181 ggtgtgatcg cgcggcccgt tcaggtgaac ggcaggcgat gttgagcgga ggccgcgcgg
      241 gcgcttgtct gaggccagcc tgtcatcccc attaggaggc ttgatgctct cgccgaaccg
      301 actgattccg ttcccgaacc ggctcgctcg gatcgcccga gcacacgaag gaccggacga
      361 cacaccgacg atggagcttt gggacgcctg ggtgttcgcc gaggtggact cgaagcttgc
      421 attgcgctcc tggtgggacg cgccgagcgg cagtaagtcc gtcgccttcg ccacctacct
      481 cgccgcgctc gaccgtgagg agcaggccgc ggccgtgctg gcggcgcggt tcgcgtgaga
      541 cggcttgtcc tgaaacctcc tggcgaacct aaggccgacg cggcgcccgc ccaaggcggg
      601 tgggcgacca gtcgatagct atccgctctc acaggcggcc ggactgagtg atcgcgagca
      661 ggcatcgacc gattgctacg ccggattgcg gtcgctcacg tggaggcgag actcccagca
      721 tcacgagtgt gctcatggag atgtacgagt gggccgcgac gcacaccgat caggtcgagg
      781 cggcgcgcga caggttcgag gtgggcttcg ccgaagccga gctcgacgac gagcggctgg
      841 cggcctgaga cgatggccgt gccctcgaac gccaccgccc cggccgacgg ccacgtcact
      901 tgggacccgc gcgcccagga gcgaaccacg cgtctgttcc ggcgccttcg ccgcttcggc
      961 gataccgcag cgcgcgacca gctgatcgag ctctacatgc cgctcgctcg gcgactcgcg
     1021 ctccggcaca cgggctcgcg cgagccgttc gacgacatga tgcaggtcgc tgtggtcggc
     1081 ctgatccttg cggtcgatcg cttcgacccc gagcgtggaa tcccgttcgc cggcttcgcg
     1141 aagccgacga tcgccggcga gctcaagcgc catctccgcg accacgcgtg gtgcctgcac
     1201 gtgccccgcg agctgcagga ccgcgcgctg cgcgtcgtgg gcgcggccga tcggttgtcc
     1261 ggtcgcggca gcgtgtccga gtcgctcgag gagctcgctg cccactgcaa gatcactgtc
     1321 gacgaagcgg cagaggcgat cgctgcttgg cacgcgtaca cgcccgattc gctcgacgtg
     1381 ccggaccccg agagcccctc acggctgcgc gtggaatcga gcggcgcgcg cgacccgggt
     1441 tacgtcaagg tcgaacacct gagcgtgatc tcgcaggcgc tcgccgcgct gtccccgacc
     1501 gagcagacca tcctggcgct ccggttcggc gccgacctga gccagaccga gatcgcggag
     1561 cgggtcggga tctcccagat gcacgtgtcg cgcctactgg ggcgcgcgct cgcgcggctc
     1621 gaagtcgtgg tccgcgcgaa cggctgagcg tggggcgatt tgggagcggg aggaacgttc
     1681 atccggtgcc tttctcccgg tcgagctgta cgaagttcgc gcacagcggg agaaacgtcg
     1741 ccttccattc cggacggcgc cttgagatgt acgaaattca cgccgcgggg gcgcaactcc
     1801 tgactttggt tctcctatgc ggaaccaacc tcaggaggtg gcgccttccc accgccgcgg
     1861 tcgcccgggc cgccggcggg gcgcgtacgc gggcacatcc atttcggatc gttgaatcgt
     1921 tcaacgaaac aatggagacg gacgtagcta ctcgcgaggg gagtcagcgg cgaggtcctc
     1981 gagcggcttc ggtagcccgc gggtgaaggc tcagccgtgc gcctgggcga tagccgcacc
     2041 gaggcgagag agctcggcgt cggagagcga ggcctccaga atcaggaaca gctcccgctc
     2101 ctcgaagcgg atgtgatcgt gcagccgatc gccgagctgc cggagcgccg agacgtcgcg
     2161 cccgccgtcc gccacggcgg cggctcgctc gcgaatgtcc tcatgctcgc gccggacgcg
     2221 ccccgtgagg tcgctccacc ccggcgtgga gtcgggcagg gccccgagca gcacgcgctc
     2281 ctcgatctcg aaatggtgcc ggccctcgcc ggtccagaac tgctcgaagt gagctgcagc
     2341 ctcggacgcc gagtcggcgc tcgcgcgacg cagccgcaga gcggcgtcga ggccgtgctg
     2401 gtggtcgtgc gacagcggga tgagggcttc ggagcgcttc acgcgcgcac ggctactcct
     2461 cgagcagcgc cgccagcttc gccggcgtct cctcgtcggg cagcggcgag aacatcgcga
     2521 cgcgctgctc gggctgctcg gccggcctga ataccgcgtg ctcgaacacg agcgttccgg
     2581 cgagtgggtg gttgagcgtc ttccggccct cgccggatcc cgcaacctcg tgccgcttcc
     2641 accacttgcg gaattcggga ctcgagtcgt taagagcggc tatcagctct tcgaaggcag
     2701 ggtcgccgat gtgcttcgcg ctgtcggcgc ggaagcgggc gaccatcagt cgcgcgccct
     2761 tgttccagtc ggttatgagc tcgcggcggg ccgggtccat gaacgcgagc cagagcgtgt
     2821 tgcggaacga tttgggggtc tcggccgggt cgccgaagac ggcactgtag gcgcggttcc
     2881 aagccaggag gtcccagcgg cggccgatca cgtgagccgg gttcggcccc aggttctcga
     2941 tcaggcgccg tacggcgggc gagaccttct cggccggcga gcacagcgac ggcgcctgct
     3001 cgccgcggcc gagcaggatc aggtgattgc gctccgcggg ggtcagccgt agagcccgcg
     3061 cgatcgactc gagcacgtcg agcgacgccc gcacgtcgcg gccctgctcg agccacgtgt
     3121 accaggtggt gcccacgccc gcgagcagcg ccacttcctc gcgccgcaga cccggcgtgc
     3181 gccgcctgcc gccgttcggc aagccgacct cgtcgggctg gatcgcctcc cggcgcgtgc
     3241 gcagaaagtc cgccagctcg tcgcgccgga tctctccgtc gctgccgttt cgctgtcgcg
     3301 tgatgctcgt ggggggagtc tctactacca ggataaatcc ctctcttgta cctggatcaa
     3361 catgatcgca cactggggcc atgcccgcaa tcgcaaagct tcccctccgc cgccaggcaa
     3421 ccagcgacag acgcgatctg aaccttctcc tcgtggtcct ctgcttggcg cagttcatgg
     3481 tcgtgctcga cgtgtcggtc gtgaacgtcg cgctgccgtc gatccgccgc ggcctcggct
     3541 tctcggcaac cgacctcgag tgggtgatca acgcctacac gctgacgttc gccggcttcc
     3601 tgatgctggg cggccgcgcg gcggaccttc taggacgccg gcgcatgttc ctcgccggca
     3661 tcgcgctctt cacgctcgcg tcgctcgcct gcgcagcatc ggactcgcgc gcgctgctcc
     3721 tcggtgcgcg cgccgtgcag ggcgtgggcg ccgccgtcgt ctcgccagcg agcctgtcca
     3781 tcatcgccac gtcgttcgcc gcgggatccg agcgcaaccg ggcgctcggc gtctggggcg
     3841 cgatggcggg tctcggcgcg gcatccggag tgcttctcgg cggggtcctc acgcagacgc
     3901 tcggctggtg ggcgatcttc gccgtgaacg tccccgtcgg cgcggcggtc gtcgcgctcg
     3961 cgccggccgt agtgccggag ggtcgccgcg cgggcgctac gcgcaacttc gacctctcgg
     4021 gcgcgctgct cgtgaccgcg ggcctgacgg cgctcaccta cggcatcgtt cgcagtgacg
     4081 cgctcggctg gacctcggct ggagtgctca cgccgatcgc caccggcgtc ggcctgctcc
     4141 tggtgttcct gttcgtagag gggagactcg cgagtgcgcc gctcgtgccg ctctcgatct
     4201 tccggttgcc gctgctgcgc gcggcgaacc tcgtcgtgct gctgatgtac tcgggcacgc
     4261 tcgcgatgtt cttcttcgtg agcctgtacc tacagcaggt cctgcatttc gacgcgatcg
     4321 agaccggcct cgcgttcctg cccatgacgc tgttcgccac tacgggctcg acgctcgcgc
     4381 cgcgcctggt cgcccgcttc ggagcgcgca gagtcgtgac cgcgggcatg ctgtgcgtgg
     4441 ccaccggcct ggcgctgctc accgggatcg cgccaggtgc gagctacgcg ctgcacgtct
     4501 tgccgggtgg agtgctcacc acactcggac ttgggctgac gctcgtgccg tcgacgatca
     4561 tggcggtgca gggcgtagcg cccgaggaga gcggtgtcgc gtcaggcctg ctcaacacgt
     4621 cgcgcttcgt cggtggcgcc cttgggatcg cagtgctcgg cacaatcgcc gcgtcgcaca
     4681 cgaggcatgt cgcagaggcg ggcaccggag cgcttcgggc gctgagcgac ggctaccagc
     4741 tgggccttgg gatcggggcg atcgtgtgcg tgatcggcgc ggtcatcgct gccacattcc
     4801 tccgcgaacg cccgcagccc gaacccgcga ccgtccccgc cgagggcgag gaggccgagg
     4861 ccctcgcggc ctgaggcccg atggtgtgcg gttagccgta ctgtggtaga ggcggcaccc
     4921 gtttagggtc acgccgcccc aactcaccgg caccgcaggg cacggtgcag gaaaggacca
     4981 gatgagtcac cgcaggatct ttggcggcgt gctcgggcta gccgtcgcca tcgccggtac
     5041 cggtgtcgcg tttgcggcgc accccgccgc ggcgcccaag aacgtcacgg tcaaggagaa
     5101 gttcagcatg acgatgaagc caaatcgctt catccaggac ggaatgcgct tcgacaagga
     5161 cgtctacacc gtcaagtcgg gtggcaagct cacgttcgtg ctgaaccagg cccaggaagg
     5221 gccgcacacc ctgtcgatcg tccgtccgaa ggaccttccg aagacggcgc agcaggtcaa
     5281 caagtgcaag atctgcgaga agctcggaaa agctcacggc gcggacccga actcggatgc
     5341 gccgccgaag ttcccgttcc tcgagaacgg ggtgggccag gacacgccgc cgaagctcga
     5401 caagccgggc gactccggcc tcgtcgggcc gaacaggggc gccaaggtgt cgttcgcggt
     5461 gacggcgaag ccgggcagca cgctcacctt catgtgcctg atccacccgt ggatgcaagc
     5521 gaagctgaag gtcgtcaagt aggagcgcgt tgaagctctc gaagctcgca agggccctgg
     5581 cggcagccgg ggcccttgcc gcgctcatcc ccggcgcggc gcaggcgcgc gtgctcgact
     5641 actgggtcgc ggcggtgccc acgcagtgga acatcgtgcc gaacggccgc gacgcgatca
     5701 tgggcatgcc ggtcgatccg gccgacacga tcttcccgac ggtcgtctac cgccgctaca
     5761 gcccgcactg gcgaaggcag ctcgcgaacg ccgagcgctc ggccgccgac gggctcgcca
     5821 tcccggggcc gctgattcgc gcgcgagtcg gcgaccgcct gcgcatccat ttcaagaaca
     5881 tggacacgct gcggcacgac ccgcactcga tgcatttcca cggggtgcac tacaagccga
     5941 gctcggatgg tgcctacgtg ccgggcttct ccggcccgga cgccgatgtg aaaccgggcc
     6001 gcctctatac gtaccgcctc acggccgggc gcgactcggc cggcgtctgg ccctaccacg
     6061 accactcgcc ctcgatggag cagtcgatcg ggggcgggct gttcgggatg ctctcgatcc
     6121 tcggacggca cgagcggcga gcagaccgag agttcgaggt cgtgttcacg cccttcggca
     6181 agttcatgtc gatcgacggg cgcgcgttcg tggggaacac gcccgtgttc aagtccaagg
     6241 tcggcgacct cgtccagtgg gacgtgatgg ccatggctcc gagcaccaca cattccatgt
     6301 tcacggccac cgctggatcg gcgcggacgg cgtgccgcgg gacacgcaga ccgtcggtcc
     6361 ggccgagagc ttccgattcc gctggatcga ggaggacccc ggaacgtggc tctaccactg
     6421 ccacgtcgag gatcacatga tgcgcgggat gatcggcatc taccgcgtgt cgaagcgatg
     6481 aggtcgcggg cgctcgccgc ggcgctctgc gccgtgggca ccttggcggt tgcggctccg
     6541 gcatctgcgg ccacccacga ggtgcacgcc ctcttcgcgg agttctcgcc caccccgctc
     6601 gacgtcctcc cgggcgagac cgtcgagtgg atgaacatga gtgagcgacg ccatacggtg
     6661 accgccgacg acggctcgtt cgaatccggc gaccagttca acccgggcgc cagcttcagc
     6721 cagaccttcg actcggtggg cacctacctg taccactgcc gccttcacgc gggcatggtc
     6781 ggggagatcg acgtgcgccg ggtgaccctc gacccgctgc ccacggctcc ggttccgcag
     6841 ggcgacgcgc tggcgctgag cggacggacg gcgacgccgg tgcagccggt gacgatcgag
     6901 ctggacagcg gccacggctt cgccccggtc ggcacggcga cgccctccgc tgacggcagc
     6961 tggaaggcga ccgtgaaggc ccagcagacg ggcgactacc gcgcggtcgt cggcgcggac
     7021 acgagcgaga gccggcggct ggtggtgagt gatcgcaaga tcacgattcg cgtgacacgg
     7081 accggcgtgg cggtgagcgt ccgcccggcg cttccctatg cgcgcgtcgt gctccagcag
     7141 tacctacgcg agcgcttcgg ctggtggccg cagaagcgcg ggcgcttgga ctacgtctcg
     7201 actgcgagct tccgcgtgag ccggcccgcc cgtgtgcgcg tcgcgctgct gggctccgac
     7261 gattggaccc cgctggtcac gagcaaagtc gtgacgctga agcgccgcgc tagcggctct
     7321 cgagccagtc gctcacgtcg ctcggcggat ccccgtgcca ctgccgccgc accgcgagct
     7381 cgatgagctc gtcgggatcc cggtcgtagt tcgcccttcc gcgctcgtga gctaggtaca
     7441 gcaccaccgc cgtagcgacc gacacggccg ggtcgtcgct ctcgtagtcc gcgccctcgg
     7501 cggcaagcgc cagccgctcc tcgaggttct cgaccgccgt gatcggctcg tcgtcgtcct
     7561 cgagctgctc ctcgaagtgg ctgagagact cgtcgtcgcc ggccgggtcc gtcacctcga
     7621 ctgcctggcg cacgagctct gacacggtgg ccgttgggcg gtccatgacg ccgaagccta
     7681 cgctccacgc cgtgactgca cgagggtttg cgttgttcgc ggcggtgtcg ctcgtctggg
     7741 gcctgccgta cctgttcatc aagatcgcgg tgaacggggg tttctcgccg tcgggtgtgg
     7801 cattcgtgcg tgtggcgctt gctgcggccg tgctcgtgcc gatcgccgtg tggcgcggcg
     7861 gagtgcgcga gctgcgcggg cgctggcccg cggtgatggc gttcgcgctt ctcgaggtgt
     7921 gcgtcccctt cccgctgatc gctttcggcg aacagcacgt ggcgagctca ctcgcggcga
     7981 tcctcgttgc cacgctgccg cttgtcatcg cggtcctggc gatccgcctc gacccggagg
     8041 aacgcgccac cggcgtgcgt ctcgccggcc tgatcgtggg ccttgtcggt gtggctttgc
     8101 tgctcggcgt cgacgtcgcc ggagatggca aggagctgct cggggccttc gcgatcctcg
     8161 tcgcgaccgt gggctacgca ctcggcgcga tgatcgtcaa gcgatcactc agcgatctgg
     8221 acccgctcgc gccgatcgcg ggttcgctgc tcgtggcgtc cgtggtgctc gcgccggcgg
     8281 cggcgctgga ctggccgtcg cgctcgccga gcgccggcac gcttggcgcg ctcgccgtgc
     8341 tcgggctggt gtgcaccgcg ctcgcgttcc tgctctattt cgcgctgatc gccgaggcgg
     8401 gaccgagcaa ggcctcggtg atcacctatc tgaacccggt cgtggcgctt gcgctcggag
     8461 tgatcttcct cgacgagtcg gtgtcggcgg ccacggcagc cggcctggcg ctcatcctgg
     8521 ccggctcgtg ggtcgccacg ggcgggcgca gggaaacgcc gagcgccgtg gccgttgaac
     8581 cgaaaggagt ggcgtgagca acgaaacttt cgacgcggtg cagattggga cggtccactc
     8641 gccgctcaca gacacagctt cggcgccgaa gcagggtcgc gagggagcgc cagaggccgc
     8701 gatcgagttc agcgcagacg tactcgacgc cctcgagagt ctgcgcgtgg gcgaccgcgt
     8761 gatcgtcgtc acctggctgg accgcgcccg ccgagacgtg ctgcgcgtgc atccgcgcga
     8821 caatcctgcg aatccgctgc ggggcgtctt caacaccagg tcggctgacc ggccgaatcc
     8881 gatcgggctt cacgaggtgg agatcgtcgc gatcgacggg aacaccgttc gcgttcgcga
     8941 cctcgaggcg atcgacggca ctccggtgct ggacctgaag cctgtgatct aggccgattc
     9001 acccccgctg ggtgatgcgc gtcgcgggcc tgagcgctca cgatcgacgc agtgatggac
     9061 tggacgctcg ccttcgtggc gctcgccgtg ctgattgtcg cggccgtgtc cggccgtctt
     9121 tccgacggac cggtcacgcc cgcgatggtc ttcgtgggca tcggggtgct gatcgggccg
     9181 cgagttctcg acgaagtcga tctctcgcct ctcagtgcgc cggttcgcac gctcgcagag
     9241 gccgcgctga ccgtcgtcct gttcagcgac gcgtcgcgga tcgacatgcg ggcgctacga
     9301 cgcgagtacg gcctcccggt gcgactgctc gcgatcggcc tcccactgac gattgcagcc
     9361 gggaccgcgg ccggcatcgc ggtgtttggc ggactcacgg cgctcgaggc cctggtgctc
     9421 gcggtcgttc tcgcgccgac tgatgcggcg ctcggacagg cggttgtgac cgagccgcgg
     9481 ctgcccctgc gagtgcggca gggcctgaat gtcgagagcg gcctcaacga cgggatctgc
     9541 gtccccttgc ttctgatcgc gctcgccgga gcggacgtga actcccacat cgacagcggc
     9601 cacgatgcgc tcaaagtcgt gttcgaggag atcggctacg gcctgctcgg gggagtctcg
     9661 gcgggtgtcg ctgccgccgc gatcgtggtg atcggccggc gtcgcggtct gatcgccgac
     9721 tcctggctcc cgatcgtgcc ggtcgccgcg gccgcgctcg cgtacggcgt ggccaccccg
     9781 ctcgaggggt cgggcttcat cgcggcgttc gtcgcgggga tgtgcttcgg agcgctgctc
     9841 cgccgtgagc cccagccgat ggaggccttc ggtgaggaga tcggccagct gctgagcggc
     9901 gtcaccttcg tggctttcgg agccgtactt ctcggccccg ctctcggcga cctgagctgg
     9961 cggattggct gctatgccct cctctcgctc acggtcgttc gcatgcttcc cgtcgcgctc
    10021 tcgctgatgg gctcaggagc gcgtcggcag accgtcgcct tcctgggctg gttcggtcca
    10081 cgcgggctcg cctcgatcgt gttcgccgtg atcgtgctcg aggagtcgaa gctgcctcac
    10141 ctgaacacca tcctgctgac cacctacgcc accgtcgggc tgtccgtgct gctgcacggc
    10201 ctgagcgccg ccccgctggc acgggcctac gccggctggc accaacgcca cccacgaaaa
    10261 gacgatctgt agggcagcgg cgccgcggcc gataacaggc acgtggggga gcagtcgccg
    10321 gacaccgagc cgcgtccgga cggccagatg gcctgttcca tggcgagcgt gctgatccgg
    10381 cgcgtgcgct cgtcgctggg cgaggccgcc gtccagcagc tgatcgagcg ctccggcgtg
    10441 ccgtacgact cggagtacct cgaggacctc ggcaactgga tcccgtacga gcatgcgggc
    10501 gcgctgatgg aggcggccgt gaaactcacc ggcgacgagg acttcgtgcg ccgcgccggc
    10561 gaggacacgg ttcgccagca cgccggcacc gcggtcgcga ccctgcttcg gtcgctcgga
    10621 tcgccgcagg cggtgttcga gcagctgacg ctcgccgcaa cggagttcag caccgtgatc
    10681 gacctggcac ccgtcgaggt cgcgcccggc cgagcggtcg tccgcgcgaa ggcgaagccc
    10741 ggttacgcgc gccatcgccg ccactgcgag tacacgcagg ggatgctgtc ccagccgccg
    10801 gcgcttttcg gcctgccggc cgctcgtgta gtggagtcga gttgtgaact tcacggcgac
    10861 gaccactgcc tctacaccgt cagctgggac gccgaaggcg cggagcgcgc gaccgatccg
    10921 ggcgagctgg tcactgcgct cgagcagcag gtcgccgcga tgacagaccg cctcgagaac
    10981 atgtacgcga ccgcccgcga tctcatcgcg ctcaacgacg tggacgccgc gctcacccgc
    11041 atcaccgagc gcgcgtgcca cggctgtccg tgcgccgagc tacctgctcg ccgtacgggt
    11101 cgggcgcggc gacgagctgc acgttcacca tcgcggcttc acggatgcgg aggccgacaa
    11161 ggcggcgcga gcgctgctgg gcgacgagag cgtcgtctcg cgcgcgtccg gactcgtcgt
    11221 cgacgttgcg tcggccactc gccattacgg caggctgatg gccgcctcgc ccgacggcgc
    11281 tttcttcccg cacgagcgcg acctgctcga cgtctacgcg cgttacgccg ccgccgtgct
    11341 cgacacggcc accgccctcg acgaggctcg ccggcgtcac gaggaggcgc aggccctgct
    11401 cggtctcgcg caatcggtcg ccacggccaa cacgagccaa gacgtcgcgg agcggctggc
    11461 gggcgcggtg ccggcggtcg tcgactgcga ccgtgtcttc gtgttcctgt gggacgaggt
    11521 ggagcaggcg ctcgtgcacc acgccagcgg aggcgatccc gggccgagcg gcgacatcgc
    11581 ccgtcagctg cggatcaccc ccgatgacac gccgcgcctc cgggagctgc gcgcctcacc
    11641 gatgccagag cccatcttct tcgacgcgca tcccgacgac ccgttcctcg aggcgaccat
    11701 gcgcgcgacc ggtgcgctcg cgctcgtggt cgtcccgatc gtcgccaacg aacgcttcta
    11761 cgggaccatg aacgtgagcg tgaccgagcg acccgagcgc cttgcgccgt cgcccgcgct
    11821 gctcgacacg ctcgccgggg cggtggcgca ggcggcgacc gcgctcgaca acgcgcgcct
    11881 gatcgagtcc atggctcacc aggcacggca gcctactcgg ccaccgtgcc ttccacgagg
    11941 cgatcgccga cggactctct gagcgcgacc ccgaggcgtt cacgctggcg atgattgaca
    12001 tcgacgactt caagcagatc aacgacacgc acggccatcc ggtcggggac gaggcgctcc
    12061 ggctagtggc cgaggcgctc caccgcgccg tgcgcgacga cgaccgcgtg ttccgagtgg
    12121 gcggcgagga gttcgccgtg ctcctgccgg gcctgaccgc ggccgacgcc accgcggcgg
    12181 ccgaacggct gcgcgaggcg gtagcgcgga tcgagttcct cgtaccactc acagtgagcg
    12241 tcgggctcgc gtcgtggccg gagggcgcct ccgaccgcga cagtctgatc gagcacgcgg
    12301 attgtgccct ttaccaggca aaacgcgccg gtaaggaccg cgtaagcgtc gcggcttagc
    12361 ggggccccgc aagtaagggg ttcgccgcct caatcggctc catatgcccg atgtgggatg
    12421 gagctcctca gtccaccatg ggtagcggcg aatgacccgt tccccggaaa ggacagagag
    12481 atgctcacct acctccgacg agacttcctc gactccagcc tcacgcccga ggcgcgacgc
    12541 ccgacgttgg cggacgagct cttcgcgcga tactgggagg gtgacgactc gagtgacaag
    12601 cgcacgcgtc gttgggcgcg agcgcgagct cgcggagctc gaagccgcgc tggctgacgc
    12661 ggccgcgggc cggccgtcga tcgccttcgt cgcgggcgaa tccggcatcg gcaagacgcg
    12721 cctgcttcgg gagttccaga ggcgggcgca ggaagccgat ccggcggctc gcgtgatctg
    12781 cggcgactcg gtcgagctcg gcgaggggga gctgccctac gcgcctctcg tcgcggcgct
    12841 tcgcccgctc gcgcgcgctc gggacccagt gctcgaatcg ctgccgccgg ctgcgcgcga
    12901 cgagctggcc gcgctgcttc cggggctggc gcccgcggcc tcggaggagc ccgtgggcga
    12961 gcgcgacgac gccgcgcagg cgaggctgtt cgaggcgctg cttcagctct ttgacgagct
    13021 cggccgtgaa cagccgctgg tcgtcgtgat cgaggacatc cactgggccg accgctcgac
    13081 acgcgcgttc ctggcgttca tggcgcgcgc gctggccaac gagcgcgtgc tgctcgtgtc
    13141 cacgtatcgc accgatgagc ttcaccgcag gcatccgctg aggccgctct tggcggagct
    13201 cgagcgcgac gccaccgcgc gccgaatcga gctacaggcg ctcgaccgcg aggagctccg
    13261 cgagctgctc ggcgacatcc tcggcgccga gcccggcgcc gatctggtag agcggctgtg
    13321 gtcgcgcagc gagggcaatc cgctcttcac ggaggagctg ctcgccgcga gcctcgacgg
    13381 tcgcggcggt ctgccgccca cccttcgcga cgcgctcatg gtgcgcatcg agcgcctggg
    13441 cgaggacacg caggaggtgc tgcgcgtcct ggccgccggg cgacgactcg accacggcct
    13501 gctgagtgat gcgagtggcc tcgacccggc tcgcctgcgc acggccctgc gcgaggccgt
    13561 cgcgagccac atcgtggccg cggaccccga gggctttcac acattccggc acgcgctcct
    13621 gcgcgaggtg gttcacgacg atctgctccc gggcgagcac tccgatctcc acctggcgct
    13681 tgcgcgcgcg ctcgaacggc gcatggagct ccagggcacg agcacgtacc tcaccgcggg
    13741 catcgcgcac cactacttcg tcgcgggcga gcagaaggcg gcgctgtccg cctcggtgaa
    13801 tgctgcgcgc gccgccgaac acgtatacgc gtacggagag gcggccgccc tcctcgagcg
    13861 cgcgctcgag ctgtgggagc gggtgcccga gcccgagcgc ctcgcgggct gcgaccgcgt
    13921 cgagctgctc aagcgagccg ccgtcgccca tcagggacac ggcgactacg cgcgccagga
    13981 gcacttgctt cgcaaggcgc tcgacctcct tgacgagcag gcagagccac gcggtagcgc
    14041 ccggctactc gagctcatgg cccgtgccca gtggtcgctc aatcgcagtg cggacgcgat
    14101 ggccagcgcc gagcacgcgc tcgagctcgt gcaagacggc gaacctggcg aggagcgagc
    14161 acggctggtc agctggcttg ccaaggcgcg catgctgcag ggccgctacc gcgagtccct
    14221 cgatgtcgcg agcgaggcaa tcacggaggc cgaggccgcc ggtgacacgc tcgccgaggg
    14281 ccgcgcccgc aacgcgcgcg gcttctcgct ggtcgggctc ggccgggtgg acgacgggat
    14341 cgccgagcta cggcgagcgg tcgagctcgc gcacaaccgt gagaacgtgt ccgagctcgg
    14401 cagtgcttac ggcaacctcg ccgacgctct caaccaggcg ggccgcacgc gcgaggcgct
    14461 tgccgtcgtg gagcgtggcc gcgaagtggt gtcggggttc tcccggcccc gcgactggct
    14521 cttggtgatg atcgccgagc tgaagtggca gctcggcgag tgggacgagg cggaggcctc
    14581 gatgccgcct tccgaacggc gttacgtggc gaccacgacg atgtacgtgc tgctacagcg
    14641 cgccacgttt gcgctcggac gtggcgatca cgccgttgcg cgccggtacc tggaagagat
    14701 cgaggaattc gtcgccgagt cgaccgagcc gcagtacctc ggcacctggg gtgcgctacg
    14761 cgcatcgctc tcgttgcgcg agggcgacat cgacgccgcg cgtgccgcgg tcgacgaggc
    14821 gctcgaccgg atcgagttct gcagcgagga cgccaaccgc atcgtgcgtc tgtcggccat
    14881 cggcgtcgcg atcgaggcgg ccgcgatcga gcgtgaacgc gacctgggcg aggacacggc
    14941 gctcgcgcaa ttccgcgcgg aggcgatggt ctcgcgtctg cgcgcaggcg cggaaggcga
    15001 ccggccggtc gagcgtgcct ggctcgccat ggccgaggca gaggggacgc gggtcgat
//
LOCUS       DASVXS010000157         5129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_799529, whole genome shotgun sequence.
ACCESSION   DASVXS010000157 DASVXS010000000
VERSION     DASVXS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799529"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VF752_08685"
     CDS             complement(<1..429)
                     /locus_tag="VF752_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8855665.1"
                     /translation="MSFDSPLVLVALIVIPLAVVWYAAEQRRRRKAAAAFAAPVLQPN
                     VAPTRPGWRRHAPMLAVLLALAVLIVAAAKPQRTVAVPVERAAIMLTTDVSGSMTAKD
                     VQPNRLVAAKRAARSFLAKVPSGVNVGVLAFNDKPTLLQTP"
     gene            complement(426..1625)
                     /locus_tag="VF752_08690"
     CDS             complement(426..1625)
                     /locus_tag="VF752_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8855666.1"
                     /translation="MRSPRHLWIGDWRADSDEARRAAEEAAERHRQAALRGEPVEAPP
                     APRKRRFTSPMVVAALIGAAIAAGAFSLGTVLTGGSNDHGPAPLAAVSSKPIKPRPGQ
                     TRAGAIYAAASPAVVSVRTGQGSGTGFLIDKSGTLVTNDHVVANNDRVIVRFGKRADS
                     IDAKVVGTDPSSDLAVLSIDPSAIPGGVKPLQFADSRAVQVGDTAIAIGNPFGLDRTA
                     TEGIVSGLGREIQAPNGFTISEVIQTDAPINPGNSGGPLLDDGGKVIGVNSQIETGGS
                     GGGNVGIGFAVPSNTVRQVVPRLEKGETIKRGYLGISTSAVSPNNPDGARVEKVEQGQ
                     PGERGGVQVGDVITKIDGRTVADSSDITAAVSAHAPGDKVKIEIERNGQHKTLTVTLG
                     TRPARTP"
     gene            1791..2654
                     /locus_tag="VF752_08695"
     CDS             1791..2654
                     /locus_tag="VF752_08695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8855667.1"
                     /translation="MASAASTRARLEFAALALALLIALGAFAPAAVASEYVALGDSYT
                     SGPFIPQAIPPWGCLKSDHNYAHLAAPRLGLTLEDRSCNGAHTDNMTRSQAVWPEPNP
                     AQFDALDSGTRLVTLGIGGNDIDFAGIALSCVSLTRSDASPCMDRYTAGGIDQISRRI
                     ADTGPKVAGVLQAIHARAPSARVLVVNYPAIFPGRGPGCFPQLPVADGDVPWLRNKEK
                     ELNRMLAVEAAANGASLVNRYRASLRHDACELPGVRWLEPVLPASPALPIHPNLAGMT
                     AAADLVVAAAD"
     gene            complement(2949..3839)
                     /locus_tag="VF752_08700"
     CDS             complement(2949..3839)
                     /locus_tag="VF752_08700"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX8855668.1"
                     /translation="MFEEAQLYSPVTAEDGEVTVHLAHDHPGANDPEYRRRRGDIAAA
                     ALAWEPGSPAPRIDYNDAEQEVWRTVCRELAPKHRRYAVREYREALERVALPTDHVPG
                     LDEVTERVQPLTGWRYVPAAGLVPLREFYGSLENRVFHSTQYVRHPDVPLYTPEPDII
                     HEVIGHGHLLATPTFGELHRLAGVATNRLEDEDNVRFLSKVFWFSLEFGVVVEDGELR
                     AYGAGILSSYGEIEEFRGMEHRPLDLVEMGTVDYDITAYQPVLYRAESVEEIVDVVGG
                     FFATCTDASIEQMRARAPVS"
     gene            3966..4454
                     /locus_tag="VF752_08705"
     CDS             3966..4454
                     /locus_tag="VF752_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8855669.1"
                     /translation="MTIDELDARLIAALREHPRVGLLEIARQLGVARGTAQARLAKLE
                     ARGVITGHGPEVDPSKMGYTISAFMLVELAQGRLAEAVESLEELPEVLEADAISGPQD
                     LICRVVAHDTEHLQEIVNRLLANPAIRRCTSHIVLSRQVPPRTGPLVNAAANPQIDTA
                     AR"
     gene            4510..4950
                     /locus_tag="VF752_08710"
     CDS             4510..4950
                     /locus_tag="VF752_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855670.1"
                     /translation="MAYPPRLVGINHVALEVGDVEEALAFYGRIFMLGRVGREPGMAF
                     IEMGDQFIALSEVAAPSPDDARHFGLVVDDKQAVRDILAAEGVEVLPGPRLDFRDPWG
                     NRVQVVEYSDIRFTKVPSVLRGMGLEDLDKSPEALQELRDYGLL"
BASE COUNT          787 a         1748 c         1820 g          774 t
ORIGIN      
        1 aggggtctgg agcagcgtgg gcttgtcgtt gaacgcgagc accccgacgt tcacgccgct
       61 cggcaccttc gcgaggaacg agcgcgccgc gcgcttcgcc gccacgagcc ggttgggctg
      121 gacgtccttg gccgtcatcg agccggacac gtcggtcgtg agcatgatcg ccgcgcgctc
      181 gaccggcacg gcgacggtgc gctgcggctt cgcggccgcc acgatcagca ccgccagcgc
      241 gagcagcact gcgagcatcg gcgcgtggcg ccgccagccg gggcgcgtcg gcgccacgtt
      301 cggctgcagc accggcgccg caaacgccgc ggccgccttg cgccgccgcc gctgctcggc
      361 cgcgtaccac accacggcca gcgggatgac gatcagcgcg actagtacga ggggcgagtc
      421 gaagctcacg gcgtgcgggc tggacgcgtg ccgagcgtga ccgtgagcgt cttgtgctga
      481 ccgttccgct cgatctcgat cttcaccttg tcgcccgggg cgtgcgcgga gacggcggcg
      541 gtgatgtcgc tcgagtccgc cacggtcctg ccgtcgatct tggtgatcac gtcgcccact
      601 tgcacgccgc cgcgctcgcc gggctggccc tgctcgacct tctcgacgcg tgcgccgtcc
      661 gggttgttgg gcgacaccgc ggaggtggag atgccgagat aaccgcgctt gattgtctcg
      721 cccttctcga ggcgcggcac cacctggcgc acggtgttcg aggggaccgc gaagccgatg
      781 ccgacgttgc cgccgccgct gccgccggtc tcgatctgcg agttcacgcc gatcaccttg
      841 ccgccgtcgt cgagcagcgg gccgccggag ttgccggggt tgatcggggc gtcggtctgg
      901 atgacctccg agatcgtgaa gccgttcggc gcctggatct cacggccgag gccggagacg
      961 atgccctcgg tggcggtgcg gtcgaggcca aaggggttgc cgatggcgat cgccgtgtcg
     1021 cccacctgca ccgcgcgcga gtcggcgaac tggagcggct tcacgccgcc ggggatcgcc
     1081 gaggggtcga tgctcaggac ggcgaggtcg ctcgagggat cggtgccgac gaccttggcg
     1141 tcgatcgagt cggcgcgctt gccgaaccgc acgatcacgc ggtcgttgtt agccacgaca
     1201 tggtcgttcg tgacgagcgt gccgctcttg tcgatcagga agccggtgcc cgagccctga
     1261 cccgtgcgca ctgagacgac ggcgggcgag gcggcggcgt agatcgcgcc cgcgcgcgtc
     1321 tggcccgggc gcggcttgat cggcttgctc gagacagcgg ccagcggtgc gggaccgtgg
     1381 tcgttcgagc ccccggtgag cacggtgccg agtgagaagg cgccggcggc aatggcggcg
     1441 ccgatcagcg cggccacgac catgggcgac gtgaagcgcc gcttccgcgg ggccggggga
     1501 gcctcgacgg gctcgcctct cagcgcggcc tggcggtgac gctcggcggc ttcctcggcg
     1561 gcgcggcgcg cctcgtccga gtcggcgcgc cagtcgccga tccagagatg tcggggcgac
     1621 ctcatctacc tatcaaaggt acgagggccg tctagtgggc ggggcatgcg acgagtgaat
     1681 acgggggcgc ctaagaggat ctaaccccga gcgaagaatc gcgtaagagc gtgtgctggc
     1741 tcgcggcgga agttacgatc gttcgcaagg cgcaaccgga ggagatccgc ttggcttcgg
     1801 cagcttctac acgcgcgcgc ctggagttcg ccgcgctcgc actggccctg ctgatcgcgc
     1861 ttggcgcgtt cgcgccagcc gccgtcgcca gcgagtacgt cgcgctcggc gactcctaca
     1921 cgtccgggcc gttcatcccg caagcgatcc cgccgtgggg ctgcctgaag tcggaccaca
     1981 actacgcgca tctggcagcg ccgcggctcg gcctgacgct cgaagatcgg agctgcaacg
     2041 gcgcgcacac ggacaacatg acgaggtcgc aagctgtctg gccggagccg aacccggcgc
     2101 agttcgatgc gctcgactca ggcacgaggc tggtcacgct cggcatcggc ggcaacgaca
     2161 tcgacttcgc gggcatcgcg ctgagctgcg tcagcttgac gcggtcggac gcgtcgccgt
     2221 gcatggaccg ctacaccgcc ggtggaatcg accagatcag ccgtcggatc gccgacaccg
     2281 ggccgaaggt ggccggcgtg ctacaggcca tccacgcgcg cgcgccgtcc gcgcgtgtgt
     2341 tggtagtgaa ctacccggcg atcttccccg ggcgcgggcc tggctgtttc ccacagctgc
     2401 ctgtggccga cggcgatgtg ccgtggttgc ggaacaagga gaaggagctg aaccggatgc
     2461 tggccgttga ggcggctgcc aacggcgcct cgctggtgaa ccggtacagg gcgagccttc
     2521 ggcacgacgc ctgcgagctg cccggcgtgc gctggctcga gcccgtgctg cccgcctcac
     2581 ccgcactccc gatccacccg aacttggcgg ggatgaccgc ggcggcggac ctggtggtgg
     2641 ctgccgcgga ctgaacgcgg catccacttt ttcggtgttg cggagcgcaa ctcggatcgt
     2701 tgcggagcgc aactccgaaa aaaacggatg aggccctccc gcgcgatccc agcggtgtcc
     2761 gttttgacgt tgatcgattc aacgatcaga gcgaagaggg gcgctgcccc tcgggcaggc
     2821 gcctgcaggg gcggtcggta ttgatcgttg atcgattcaa cgatcaaaaa aaggaggtcg
     2881 cgctcgtccg cgcccgaacc gcgtgcgccg gagctggagg taggccgggg gccgtgggcc
     2941 ccggggccct aggagacggg cgcccgcgcc cgcatctgct cgatcgaggc gtcggtgcac
     3001 gtggcgaaga agccgccgac cacgtcgacg atctcctcga ccgactccgc ccggtagagc
     3061 acgggctggt aggccgtgat gtcgtagtcg acggtgccca tctccacgag atcgagcggc
     3121 cggtgctcca tgccgcggaa ctcctcgatc tcgccgtagc tcgacaggat gccggcgccg
     3181 tacgcgcgca gctcgccgtc ctccaccacc acgccgaact cgagcgagaa ccagaacacc
     3241 ttcgagagga agcgcacgtt gtcctcgtcc tcgagccggt tcgtggccac gcccgccagg
     3301 cggtgcagct ctccgaacgt gggcgtggcg agcaggtgcc cgtggccgat cacctcgtgg
     3361 atgatgtccg gctcgggcgt gtagagcggg acgtcgggat ggcgcacgta ctgggtggaa
     3421 tggaagacgc ggttctcgag cgacccgtag aactcacgca gcggcacgag cccggcggcg
     3481 ggcacgtagc gccagccggt gagcggctgc acgcgctcgg tcacctcgtc gaggcccggc
     3541 acatgatccg tcggcagcgc gactcgctcg agcgcctcgc ggtactcgcg caccgcgtag
     3601 cggcgatgct tcggcgcaag ctcccggcag acggtgcgcc acacctcctg ctctgcgtcg
     3661 ttgtagtcga tccgcggggc gggcgacccg ggctcccagg cgagcgcggc ggcagcaatg
     3721 tcgccgcggc ggcgccggta ctcggggtcg ttcgcgccgg gatggtcgtg cgccaggtgg
     3781 acggtcacct cgccgtcctc ggccgtgacc ggcgagtaca gctgggcttc ctcgaacatc
     3841 gttactcccc ctcgaagagc tgtccctcgc agtgcagcac ttgagggatg cgcctacaag
     3901 tgtgaccatg tgaattgggc aaaacgccca gtcatgacag cgaacgaccg ccaaagctgg
     3961 tcaatttgac catcgacgaa ctcgacgccc gcctgatcgc tgcactgcgc gagcacccgc
     4021 gcgtcggcct gctcgagatc gcgcgccagc tcggcgtggc gcgcggcacc gcgcaggcgc
     4081 ggcttgcgaa gctcgaggcg cgcggagtga tcaccggcca cggccccgag gtcgacccct
     4141 cgaagatggg ctacaccatc agcgcgttca tgctcgtgga gctcgcgcag ggccggctgg
     4201 ccgaggcggt cgagtcgctc gaggagcttc ccgaggtgct cgaggccgac gcgatcagcg
     4261 ggccacagga cctgatctgc cgggtcgtgg cccacgacac cgagcacctc caggagatcg
     4321 tgaaccgcct gctcgcgaac ccggcgatcc gccgatgcac gagccacatc gtgctttcgc
     4381 ggcaggtccc gccgcgcacc ggtccgctcg tcaacgccgc ggcgaatcct cagatcgaca
     4441 cggctgcccg gtagggcgcg aagtcgggct agccaagccg cgacacccag cctgataaac
     4501 cgtacggacg tggcttatcc accgagactc gtaggcatca accacgtcgc gctcgaggtc
     4561 ggcgacgtcg aagaggcgct cgccttctac gggcgtatct tcatgctcgg ccgagtcggg
     4621 cgcgagccgg ggatggcgtt catcgagatg ggagaccagt tcatcgcgct ctcggaggtc
     4681 gcggctccat cgcccgacga cgcgcggcac ttcgggctcg tggtcgacga caagcaggcg
     4741 gttcgagaca tcctggcggc agagggggtc gaggtgcttc cgggcccgcg gctcgacttc
     4801 cgcgatccct ggggcaaccg cgtgcaggtc gtggagtact cagacatccg cttcacgaag
     4861 gtgcccagcg tgctgcgcgg gatgggcctc gaggatctcg acaagagccc agaggcgctg
     4921 caggagctgc gcgactacgg gttgctctga tcaggtgaag agcgtgagca cgccggccac
     4981 gatgaaggcg ccggcccaca ggtggtccgt gttcacccac gcccgccgga gcacgccgag
     5041 cccgaccttc tcgtagatca ccactgcgac gacgcccatc acgagcagca tcgccagcac
     5101 gtgcagcatg accccgacgc cgctcgaca
//
LOCUS       DASVXS010000158        19018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_537932, whole genome shotgun sequence.
ACCESSION   DASVXS010000158 DASVXS010000000
VERSION     DASVXS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19018
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537932"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VF752_08715"
     CDS             <1..485
                     /locus_tag="VF752_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8855671.1"
                     /translation="ATRAAGTVIGGSEGLDLLEEAVAAVEHSPARLEATYAFADLGAE
                     LVKQRRRREGREVLRLAIERAVACGATALADRIRGDLGAGGGRPPRLELSGVDALTPA
                     ERRVCDLAARELTNREVAQRLFVTEKTVELHLTNAYRKLGIRSRFQLASAIPPAVASD
                     "
     gene            596..2968
                     /locus_tag="VF752_08720"
     CDS             596..2968
                     /locus_tag="VF752_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8855672.1"
                     /translation="MTGPLYHLGGYCSRHWKVVIATWIAAAIALAYLGHLAGDHTSDN
                     LKLPGTGSTKATNVLQAHLPDEANGSNPLVIQSSDGKLTDSKNKKAVDETYDQVLKQP
                     HVIRAVNPLDDNGSAFLSKDKTIAYIPVTMNVSQGDITKSQAQDVLDAGDPARNAGMD
                     VSLGGYAGQELSKPSTHTSEVIGLAAAVVILLFAFGTATAMALPIVTALIGLVSALSL
                     IRLLGLLIDVPTVAPTLATMIGLGVGIDYALFIVTRHKLQLKEGMRIDESIARATATS
                     GGAVVFAGTTVIIALCSLAFAGIPLVSTLGFSAAIAVVVAVLAAVTMLPALLGGLGPR
                     INALRVQFGRTHPDDHEPHGWARWARGVAKHPWPALVAGTAVLVVLAIPVLHLELGQS
                     DVGALPKSTTARQAYDGITKGFGAGTNGPLLVSAQLGKAAKPDQDNLNKIDQQQKQLD
                     QKQQQIEQQALAAGASQQQAQSEAKQQTQSQQQQLDDKKKQAENPASDPRLTTLSDDM
                     AKASDVKTVSPPTLDKSGHYAVYTVVPDSAPSSDTTVDLVNELRDTTIPKATKGTDVT
                     ANVGGQTAGYIDLASRISDKLPMIIAIVVGLSFLVLTLAFRSLVVPAKAAVMNLLSVG
                     AAYGVLTFIFQDGHGATVIGLEHGIPIVSFVPLLMFAILFGLSMDYEVFLLSQMEERY
                     RETRRPTRAVIEGLAHTGRVITSAALIMVCVFTSFVLNGDPVVKEFGVGLAVAIAIDA
                     TIVRCLLVPAVMTLLGKASWWMPTWLGRRLPRISIEGEEYFAKRPVGTAT"
     gene            complement(3008..4111)
                     /locus_tag="VF752_08725"
     CDS             complement(3008..4111)
                     /locus_tag="VF752_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8855673.1"
                     /translation="MSVAELQELEEVKLLVTKGQQTGVLTFAEIATALAEVDLDESDI
                     EDLHGFLEKSEIELVEDIDQTLPSAQEERAIDRSSRRRKPKAQLDLRPDMTTDSLQLF
                     LKDIGKVRLLTAQEEVDLAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLDL
                     IQEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLNK
                     IGRAERKLVTELGREPTADEIADVTGIEPDEVESIKRSAQAPVSLEKPVGDEEESEFG
                     QFIADERAESPYERAAEILTKEALREALENLSYRERRVLELRYGLGGEHPRTLDEVGR
                     TFNVTRERIRQIENQSLKKLQSLAEAQKLRDVA"
     gene            4423..4776
                     /locus_tag="VF752_08730"
     CDS             4423..4776
                     /locus_tag="VF752_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855674.1"
                     /translation="MSADGDKGVMGNLPRSRPGVRSEKRASSRAAKGTTAKPKAAPKP
                     RTTARTKPAASANAKPGVRSSAASRAAAPPPPPPPEAGRRGPDPVTAAVQVAETGIKV
                     ASKVAGGILRRLPRP"
     gene            4780..5664
                     /locus_tag="VF752_08735"
     CDS             4780..5664
                     /locus_tag="VF752_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8855675.1"
                     /translation="MTEAAPTRETRVPVRRRIWRGVKAFYSKAYADNVTGLSGMVAYN
                     LLLSIFPLALLALFVAGRVLRSADVEASVLHDLSRLFPSAAESTLRDLLSNLRTSSAS
                     VGLLALLASIWIGSSFWGALDTAFCRIYHVKCRSWVEQKRFALVMLIVVILFMAATVA
                     IPAAQSLVTSSADNLPFGLSTVKRIIYVATLVAGLALTFAILCVIYWSVPNRQVPWRA
                     IWPGAALATVAIAIVDWGFPLYLNNINTIAQFGTTFVFVLIVLIWFYVLALVLLSGGI
                     VNAMRFELHDTGELNISP"
     regulatory      5693..5848
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            5851..6570
                     /locus_tag="VF752_08740"
     CDS             5851..6570
                     /locus_tag="VF752_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX8855676.1"
                     /translation="MTHRCKSALIAACAAGLLVVPADAPAETGSGSGGSAAPGSGGAT
                     DSGFVLSSRRSVSLGQALRIAGKDARAAGHQVRIDAQGADGQSLPAVTVAAAGDGSFS
                     ALWKPAKAGRYLLRAVLGDGQAEAGAAQAASAPLAVTVFRTVKATWYGPGFYGKKTAC
                     GQRLTRALVGIAHRTLPCGSTVAVTYRGRTLNLPVIDRGPFARGVSYDLTGAAAKALG
                     MTVTSRIGVAPIPLQQPNKGP"
     gene            6604..7236
                     /locus_tag="VF752_08745"
     CDS             6604..7236
                     /locus_tag="VF752_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855677.1"
                     /translation="MPAATAVSRLRCLVVDDHPVVRQGIRALLERELPDVEVLSVASP
                     EAALEEVEPGSTDVVVIDLRRVSGEVQETITRLQETLQSPIVVFTADGGSRMLAEALK
                     AGVKGYVRKDSPPEDLVRAIQAARSGEFYVDPALSSSLLLEEGERTLTARQREILQML
                     ADGLHTEEVANKLDLSTETVRTHTKRILSKLGAGTRTQAVAIGIRSGLIE"
     gene            complement(7498..8412)
                     /locus_tag="VF752_08750"
     CDS             complement(7498..8412)
                     /locus_tag="VF752_08750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8855678.1"
                     /translation="MLERAPLDEIQRIPTDRVVRELPELITGILTVVAEGSNSSGDEF
                     TARSQAVAERIAALSGREHPAPYDLARDIAALQSVMIGALGRELREEDGGAFVDIAER
                     LATVFGAIQAAAADELVRERSRELEWLANTDALTGLYNIRYLKQQMQQLIGVQQRYGH
                     AFAVLLLDINGLKRINDAHGHAAGDRMLVGAAAAIRATVRTIDTVVRMGGDEFCVLAP
                     HQTASGAKILAYRIGAAVEQIENDGGIQIGVSIGVVSCPQHGTDPDRLLELADAAMYH
                     AKASGERVAVGSPTDVPAPGDETSAETR"
     gene            8684..9280
                     /locus_tag="VF752_08755"
     CDS             8684..9280
                     /locus_tag="VF752_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855679.1"
                     /translation="MGSTLPRDDALAGRREAVLRRWLGIEIARATVEDLAAVPMSERL
                     VRLEDMYDAAVEVLAAPASPKEPALRDALDALLARQEPFAVVVLDAGLRTPAARAELA
                     HSADGVWAAVDGGEGRTAVLLPGIGPAAAPIVADRLRVQAWTCAGADGLLPATGTAVY
                     PDDGVTASDLLSLAHERVGSEAAGDMDAATVTPIHRAL"
     gene            9312..10496
                     /gene="glp"
                     /locus_tag="VF752_08760"
     CDS             9312..10496
                     /gene="glp"
                     /locus_tag="VF752_08760"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX8855680.1"
                     /translation="MPDLLDIAEARRRVLDACFRLAPEELEIGSALGRVLAEDVTSAE
                     RLPPFDSSAMDGFALIAGPAGELELAGESRAGAPWSGELSHGRAVRISTGAEVPAGAD
                     AVVPVERTEERDGRVTVPATAAGENVRYAGEDVREGETVLRAGETLGPAELAVLASLG
                     HARVRCGARPRVAVLVTGDELVAPGEPLGPGQIRDSNATALAAQAERAGARVVERDTV
                     RDELAATTAALGRALEAADVVCISGGVSVGPHDHVKPALRELGVEEVFWGVRLKPGKP
                     TWFGTAPGKLVFGLPGNPVSAMVTFHLFARPALRALQGGDPRDVHVRAVLDTAIERNP
                     ARDQAVRCRVDARDDGFHAEPTKDQGSHVLTSMLGAGALALVPAGEGRIEHGERVDAE
                     LL"
     gene            10527..11252
                     /locus_tag="VF752_08765"
     CDS             10527..11252
                     /locus_tag="VF752_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855681.1"
                     /translation="MQTHDPRRELERRRTARARVHLVRGPASTANEDGSVASRQEADV
                     TLPRTELDRLWSPEYLERLARTYWRFLTRVSLGLLRVLYTPGSREIVFLRRPFRLLTF
                     HAPEYKTEIDCGIVTWRINRGLLVAPNGRNKGYLRISVRRPPESLLPDDPTTTSDDFA
                     TIRVSSEVENFYPLLRGWGWFARIGRYIYALTQVRIHKIVTNAFLRSLARLDLAPSVV
                     GRLRATEDQPLELEPAEEDRQRA"
     gene            complement(11258..12283)
                     /gene="glpX"
                     /locus_tag="VF752_08770"
     CDS             complement(11258..12283)
                     /gene="glpX"
                     /locus_tag="VF752_08770"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX8855682.1"
                     /translation="MVAGEQSGAGTVRSTRPDRNLALELVRVTEAAALAAARWVGVGD
                     KISADQAAVDAMRHVLETVRMDGVVVIGEGEKDEAPMLYNGEHIGDGSPPSVDIAVDP
                     LEGTTLTAKGYPSALAVIALSQRGTMFSPGPIVYMEKMAAREEFKDLLDLDRPLGETV
                     KLFAERKGTHVRDVMVVMLERPRHEEAINEVREAGGRVRLITDGDVSAALLAVTERSP
                     VDLLWGIGGTPEGVISAAAIKCIGGAMVGRLWPRNDEERHAAIDQGYDLSEVLDTDRL
                     VSGNDCFFAATGVTDGDVMQGVRYEGARGATTESLVMRSRSGTVRRIMARHDREKLRE
                     VTGERFG"
     gene            complement(12290..12772)
                     /locus_tag="VF752_08775"
     CDS             complement(12290..12772)
                     /locus_tag="VF752_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855683.1"
                     /translation="MDVRRLNVGELVGFVGAGVLALSLFLPWFSTSCDRPGVPPGCNQ
                     FSRFHGEYGSFNAWQTFAVLDWLLLAACAAPFILAWIIVRGHQLTWRPGEVTMIVGMV
                     AFALILLNGIILGRPGDTVDMSLEWGYFVGLLGAAGITAGGLIRQAVGGRTRKPPGTL
                     "
     gene            12995..14308
                     /gene="egtB"
                     /locus_tag="VF752_08780"
     CDS             12995..14308
                     /gene="egtB"
                     /locus_tag="VF752_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX8855684.1"
                     /translation="MLARQKTTPPGGAEPLPKGDYARLFEEARRRTADLVAPVSEDDM
                     ERVHNPLMSPLVWDIGHIAAFEDLWICQHLGKLEPLHPELAEVYDATETPRKDRGTLP
                     FLRLSDAWDYMAEVRARTLSVLDRYEGDSFIWEMVLEHEHQHNETMLQALQLAPAGVY
                     FPPSHRLAASPDEAAEPPERVLIEAGPFLMGDPGVGFAYDNERPQHEVTLRAFEIDRS
                     PVTVGAFREFVEEGGYERRDWWSDEGWLWREAEGVERPLYWTEDGGQRFFDAVEPLDP
                     ALPVMHVSFHEAQAYAEYRGRRLPSEPEWEKAAAWDPRRGEHRRHPWGDSEPDWGRAN
                     LDQFSFRPDAVGAHAAGDSAYGVSGLEGDCWEWTASEFHGYPGFRAYPYSEYSEIFFG
                     GPYRVLRGGSWATRPRVVRTTFRNWDHPQRRQIFSGFRCANDVES"
     gene            14305..15282
                     /gene="egtD"
                     /locus_tag="VF752_08785"
     CDS             14305..15282
                     /gene="egtD"
                     /locus_tag="VF752_08785"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX8855685.1"
                     /translation="MSGATIRIDSHMEASGSLGSMADDVRAGLSGALMELSPKYFYDD
                     RGSELFEQITSLPEYYPTRCEREILNRRAPEIVELCDPLELVELGSGVASKTRALLYA
                     MAGADKLRRYVPVDVSEAVVTRCAEELTEAYPGLEVHGVVGDFLQHLDRLPDGERRMV
                     AFLGSTIGNLHPADRTVFLAAVRDLLGPGDRLVLGLDLVKDVRVLEAAYNDSAGVTAE
                     FNLNMLHAINAALGADFEPAAFEHVAFFEPEHSWVEMRLRASEDQRITIPGAALELDL
                     EAGDEIRTEISTKFTREGITAELADAGMAMRSFFTDERGWFGLAVAARV"
     gene            15331..16092
                     /locus_tag="VF752_08790"
     CDS             15331..16092
                     /locus_tag="VF752_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8855686.1"
                     /translation="MNLDGLGTLVAGGASGLGAATARALAQRGAKVAIADLDEDKGAA
                     LAKEIGGISFRADVTDEAEVEGAVEGAVQEFGSIGFAVSCAGIGWAERVVGKDGPAKL
                     RPFETVVRVNLIGTFNVLRLAAAAMAQNDPGEDGELGAVVMTASIAAFDGQIGQSAYA
                     SSKGGIVGLTLPAARDLSRNGIRVCTIAPGTFDTPLLAGLPEAAREALGQQIPFPQRL
                     GKPEEFAALACHIAENRMLNGEVIRLDGALRMPPR"
     gene            16110..17066
                     /locus_tag="VF752_08795"
     CDS             16110..17066
                     /locus_tag="VF752_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8855687.1"
                     /translation="MRAALVEEIGKRPVVTETAPPKQGPGETLVAVTAAPINPIDLST
                     AAGRYPGGSPEPPYIPGREAVGTVAGSDRFGEGTRVYVGAPLARNGAMAEFCACPDSE
                     VFAVPAGVDDSLAACFGVAGLAGWLPVTWRGRLEKGETVLVLGATGPVGRVAVQCAKL
                     NGAGRVIAAARDPVELERVKELGADATVRIGDVDDLVQAFRDAAEGDIQLVVDPLWGQ
                     PAAAALEALALFGRHVQVGQTASPTAEIRSGAIRTKNVEILGHTSGLAPVEVRERAYR
                     EMCEHAAAGRLTLDYEEIPLEQVREAWERQEHFPHKKLVLRP"
     gene            17164..18381
                     /locus_tag="VF752_08800"
     CDS             17164..18381
                     /locus_tag="VF752_08800"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8855688.1"
                     /translation="MPEAVIVDAVRTPIGRAFKGSLTGVRPDDLGAYVVDQLLERNPE
                     VKPEDVEDLIAGCGLPQGEQSFNIGRIITLLSRLPNSTTGTTVHRYCASSLQAIRIAA
                     HAVMAGEGDTFIAAGVEHVSRVGMWSEDGLPQLMGALAQNAPEGVDVPQANFKNDKIN
                     GEDGRTDAYIAMGMTAENVAEKYDVSRDDMDRYAQRSQERAVTAQQDGFFEREIVAVE
                     LPDGNTVAKDDGPRADSTYEKLQTLKPAFKEDGRVTAGNSCPLNDGAAAVLVMERSRA
                     EGLGLKPRARIVASATSGLEPEIMGVAPIGAIKNVLDRASMTMNDVDIVELNEAFAAQ
                     VLPIADECGIDLDKLNPHGGAIALGHPFGMTGARIMTTLLNGLETDDKQIGLETMCVA
                     GGQGMAMLIERLN"
     gene            18395..18937
                     /locus_tag="VF752_08805"
     CDS             18395..18937
                     /locus_tag="VF752_08805"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8855689.1"
                     /translation="MLHGWQGSGPDHWQTWLAGRLRALGEHVSYPQLPNPDRPVLAEW
                     RTALAAELDAARDRELVVLCHSLGGILWLHHAARPVSDVVPERVLLVAPPCAPWIAPE
                     LAAFPYAPMDRAGVARAARTTRMVCSDNDEYCPRGAANVYPPVLGIDCEVLPGAGHIN
                     TDAGYGAWPLAERFALSDAV"
BASE COUNT         2905 a         6660 c         6567 g         2886 t
ORIGIN      
        1 gtgcgacgcg tgctgccggc accgtgatcg gagggtccga gggtctcgac ctgctcgagg
       61 aggcggtggc cgcagtcgag cactccccgg ctcggctcga ggcgacgtac gcgtttgccg
      121 acctcggcgc cgagctggtg aagcagcgcc gccgccgcga gggccgggag gtcctgcggc
      181 tcgcgatcga gcgtgcagtg gcctgcggcg caacggcgct cgccgaccgc atccgcggcg
      241 acctcggcgc cggcggtgga cgaccaccgc ggctcgaact gagcggcgtc gacgctctca
      301 ctcccgccga acggcgtgtc tgcgacctcg cggcgcgcga gctcacaaat cgcgaggtcg
      361 cacagaggct gttcgtcacc gagaagacgg tggagctgca cctcacgaac gcctatcgca
      421 agctcggcat tcgctcgcgc ttccagctcg cgtcggcgat tccgcccgcg gtcgcctccg
      481 actgaccctt acgccccccc ccagcaaata ctgggggaaa gcctggggac cctcccattg
      541 tgccgccctg cggccggaac caagctacga ccgacagcaa ggaaaggtcg ccggaatgac
      601 tggtccgctc tatcacctcg gcgggtactg ctcgcgccat tggaaggtcg tcatcgcgac
      661 gtggatcgct gccgcgatcg cgcttgcata cctggggcac ctcgcgggcg atcacaccag
      721 cgacaacctc aagcttccgg gcaccggctc caccaaggcg accaacgtgc tgcaggcgca
      781 cctaccggac gaggcgaacg gctcgaaccc gctggtgatt cagtcgagcg acggcaagct
      841 cacggactcg aagaacaaga aggcggtcga cgagacctat gaccaggtgc tcaagcagcc
      901 gcacgtgatc agggccgtga acccgctcga cgacaacggc tcggcgttcc tctcgaagga
      961 caagacgatc gcctacatcc cggtcacgat gaacgtcagt caaggcgaca tcactaagag
     1021 ccaggctcag gacgtcttgg acgcgggcga tcccgcacgc aatgcgggta tggacgtgtc
     1081 gctgggcggc tacgcgggcc aggagctctc caagccgagc acgcacacga gcgaggtcat
     1141 cggccttgcc gcggcggtgg tgatcctgct gttcgccttc ggcacggcga ccgcgatggc
     1201 gctgccgatc gtcacagcac tgatcggcct cgtcagtgcg ttgtcgctga tcaggctgct
     1261 cgggctgctg atcgacgtgc cgaccgtcgc cccgacgctc gcaaccatga tcggcctggg
     1321 cgtcgggatc gactacgcgc tcttcatcgt cacccggcac aagctccagc tcaaggaggg
     1381 catgcgcatc gatgagtcga tcgcgcgggc aaccgccacg tccggcggcg ccgtggtgtt
     1441 cgccggcacg accgtgatca tcgcgctctg ctcgctcgcg tttgcgggca ttccgctggt
     1501 gagcaccctc ggcttctcgg cggcgatcgc agtggtggtg gccgtgctcg ctgcggtcac
     1561 catgctgccc gcacttctgg gagggctcgg accgcgtatc aatgcgctac gagtgcagtt
     1621 cggccgcact caccccgacg atcacgagcc gcacggctgg gcgaggtggg cacgcggtgt
     1681 cgcgaagcat ccgtggcctg cattggtcgc gggcacggcc gtgctcgtcg tgctggcgat
     1741 tccggtcctg cacctcgagc tcggccagtc ggacgtcggc gcgctcccca agtcgaccac
     1801 cgcgcgtcag gcgtacgacg gcatcaccaa gggcttcggc gccggcacga acgggccgct
     1861 gctcgtgtcc gcgcagctcg gcaaggccgc caagcccgac caggacaacc tcaacaagat
     1921 cgaccagcag cagaagcagc tcgaccagaa gcagcaacag atcgagcagc aggcgctggc
     1981 tgcgggcgcg agtcagcagc aagcccagtc cgaggccaag cagcagacgc agtcgcagca
     2041 gcagcagctc gacgacaaga agaagcaggc cgagaacccg gccagcgacc cgcgcctcac
     2101 aaccttgtcg gatgacatgg cgaaggcatc ggacgtgaag acggtgtcgc cgccgacgct
     2161 cgacaagagc gggcactacg ccgtgtacac ggtggtgccg gactcggcgc cctcgtcgga
     2221 cacgacggtg gacctggtga acgagctgcg cgataccacg atcccgaagg ccaccaaggg
     2281 cacggacgtg accgcgaacg tcggcggcca gaccgcgggt tacatcgatc tggcgagccg
     2341 catcagcgac aagctgccca tgatcatcgc gatcgtggtc ggcctgagct tcctcgtgct
     2401 gacgctcgcc ttccgctcac tcgtggtgcc ggcgaaggcc gccgtgatga atctgctgtc
     2461 ggtgggcgcc gcctacggcg tgctcacgtt catcttccag gacggccacg gagcgaccgt
     2521 gatcggcctc gagcacggca tcccgatcgt gagcttcgtc ccgctgctca tgttcgcgat
     2581 cctcttcggc ctctcgatgg actacgaggt gttcctgctc agccagatgg aggagcgcta
     2641 ccgcgagaca cgccggccga cccgcgccgt gatcgagggt ctcgcccaca cagggcgcgt
     2701 gatcacctcg gcggcgctga tcatggtgtg cgtcttcacg agcttcgtgc tcaacggcga
     2761 ccccgtggtc aaggagttcg gcgtcgggct ggcggtggcg atcgcaatcg acgcgaccat
     2821 cgtgcgctgc ctgctcgtcc cggcggtgat gacgctcctg ggcaaggcca gctggtggat
     2881 gccgacgtgg ctcggccggc gcctgccgcg catcagcatc gagggcgagg agtacttcgc
     2941 taagcgcccc gttggcacgg cgacgtagcc gcacccacga cggtcaacgc atgttccgcg
     3001 acgtcgccta ggcgacgtcg cggagcttct gagcctctgc aaggctctga agcttcttca
     3061 gcgactggtt ctcgatctgg cggatccgct cgcgcgtgac gttgaacgtg cgccccacct
     3121 cgtcgagcgt gcgcgggtgc tcgccgccga gcccgtagcg gagctcgagc acgcggcgct
     3181 cgcggtagct caggttctcg agggcctcgc ggagggcttc cttcgtgagg atctcggcgg
     3241 cacgctcgta cggcgactcg gcgcgctcgt cggcgatgaa ctggccgaac tccgactcct
     3301 cctcgtcgcc aaccggcttc tcgagcgaga cgggcgcctg cgcggagcgc ttgatcgact
     3361 cgacctcgtc gggctcgatg cccgtcacat cggcgatctc gtcggcggtg ggctcgcgcc
     3421 ccagctccgt gacgagcttg cgctcggcac ggccgatctt gttcagcttc tccacgacgt
     3481 gcaccgggat gcggatcgtg cgcgccttgt cggcgagcgc acgcgcgatc gcctggcgga
     3541 tccaccaagt ggcgtacgtg gagaacttga agcccttgcg gtagtcgaac ttctcagctg
     3601 cgcggacgag tccgagcgtg ccctcctgga tcaggtcgag gaacggcagg ccctggttcc
     3661 ggtagttctt cgcgatcgag acgaccaggc ggaggttcga ctcgaccatc ttctgcttcg
     3721 cgtcgaggtc gccgcgctcg atgcgcttcg cgaggtcgac ctcctcttgc gcggtgagca
     3781 gccggacctt gccgatgtcc ttcaggaaca gctgaaggga atcggtggtc atgtcgggcc
     3841 gcaggtcgag ctgcgccttt ggcttgcgcc ggcgcgagga cctgtcgatg gcccgctcct
     3901 cctgcgccga ggggagcgtc tggtcgatgt cctcgaccag ctcgatctcg gacttctcga
     3961 ggaacccgtg gagatcctcg atatcgctct cgtcgagatc gacctctgcc agcgccgtcg
     4021 cgatctccgc gaacgtgaga acgcccgtct gctgaccctt ggttacgagg agcttgacct
     4081 cctcaagttc ctgaagttcc gctactgaca ttgacgtccg atctaagacg aatgcggttc
     4141 cgcgcgcaca gaaccccatt caagcatttg gatttccaag tctagaaccc ccgatagaca
     4201 ggtcaagctg cgctcgacct ccgctgacag cggtccaaag cgggcccaca aagcgcacac
     4261 tctgtacccg ttattggggt atctcacgcg caaagccgac aaatccgtga catccatcac
     4321 ggatagcccg gcgttggcgc gaaggcgaag gcggctgcac tcgccccggc gcgaagacta
     4381 tcgttcgcga cgcgtgatct cgacagttca cctgcagaca cgatgagtgc cgacggagac
     4441 aagggcgtga tggggaacct gccgcggtcc cgtcccgggg tgcgcagcga gaagcgcgcc
     4501 tcgtcgcggg ccgcgaaggg aacgaccgcg aagccgaagg cggcgccgaa gccgcgcacg
     4561 accgcgagaa cgaagcccgc ggccagcgca aacgccaagc ccggcgttcg ctccagcgcg
     4621 gcgtctcgag cagccgctcc cccaccgccg ccgccgcccg aggccggccg ccgcgggccc
     4681 gacccggtga ccgccgccgt gcaggttgcg gagacgggga tcaaggtcgc gtcgaaggtc
     4741 gcaggcggca tcctgcgccg tcttccccgg ccctagacca tgaccgaggc ggcgcccacg
     4801 cgcgagacgc gcgtgccggt gaggcgccgg atctggcgcg gcgtgaaggc cttctactcg
     4861 aaagcgtacg ccgacaacgt caccgggctg tccgggatgg tggcgtacaa cctgttgctt
     4921 tcgatcttcc cgctggcgct gctcgcgctg ttcgtcgcgg gccgcgtgct acggagcgcc
     4981 gatgtggagg cgagcgtgct tcacgacctc tcgaggctgt tcccgtcggc cgccgagtcg
     5041 acgctccgcg acctcctcag caacctgcgc acctcgtccg cgagcgtggg cctgctcgcc
     5101 cttctggcga gcatctggat cggctcctcg ttctggggcg cgctcgacac cgccttctgc
     5161 cgcatctacc acgtgaagtg ccgcagctgg gtggagcaga agcgcttcgc cctcgtgatg
     5221 ctgatcgtgg tgatcctctt catggccgcg acggttgcga tcccagcagc tcagagcctc
     5281 gtgacctcga gcgccgacaa cctgccgttc ggcctctcca cggtgaagcg catcatctac
     5341 gtcgcgacac tcgtggcggg gctcgccctc acgttcgcga tcctgtgcgt catctactgg
     5401 tcggtgccga accgccaggt gccgtggcgc gcgatctggc ccggtgccgc cctcgcgacg
     5461 gtcgcgatcg cgatcgtgga ctggggcttc ccgctctacc tgaacaacat caacacgatc
     5521 gcgcaattcg gcacgacgtt cgtgttcgtg ctgatcgtgc tgatctggtt ctacgtgctc
     5581 gcgctcgtcc tactcagcgg cggcatcgtg aacgcgatgc gcttcgagct gcacgacacg
     5641 ggtgagctga acatcagtcc ctgaccgacg tgcgcgacgg ggagcgccgc tatagtgcgc
     5701 gccgccgaat cggtcgggac ttcccgggcg atcgggggaa ccacttgccg gcccggtggc
     5761 cggcgggggc gaacggacgc atcaccgcat ccgaagcgcg agagcgcgag cccgacagct
     5821 aaccccgtag gcaccgaagc aggccaatcg atgactcatc gctgcaagag cgccttgatt
     5881 gctgcgtgcg ccgcggggct actcgtcgtg cccgccgacg cgcctgccga gaccggctcc
     5941 ggctcgggcg gcagcgccgc tcctggcagc ggcggcgcca cggactcggg cttcgtgctg
     6001 agctcgcggc gctcggtatc gctcggccag gcgttgagga tcgccggaaa ggatgcgcgc
     6061 gccgccgggc accaggtgcg catcgacgcg cagggcgcag acggtcagtc gctccccgcg
     6121 gtgacggtcg cggcggccgg cgacggctcc ttcagcgcgc tctggaagcc ggcgaaggcc
     6181 ggcaggtacc tgcttcgcgc cgtgctcggc gacggccagg ctgaggccgg tgccgctcag
     6241 gctgcatccg cgccgctcgc ggtcaccgtg ttccgcaccg tgaaggccac ctggtatggg
     6301 ccggggttct acggcaagaa aacggcctgc ggccagcgcc tcacgcgcgc tctcgtcggc
     6361 atcgcccacc ggaccctccc atgcggatcg accgtcgccg tgacgtatcg cggccgcacg
     6421 ctgaaccttc ctgtcatcga ccgtggtccg ttcgcccgcg gcgtgtctta cgacctgacc
     6481 ggggcagccg cgaaggccct cgggatgaca gtgacaagcc gaataggggt ggcgcccatt
     6541 ccgctgcagc agccgaacaa gggtccgtag cgggtcccct gttcggagta tgcttgacat
     6601 ctgatgcctg ccgccactgc tgtcagcagg ctgcgctgcc tggtggtcga cgatcacccg
     6661 gtggtacgcc agggaatccg cgccctgctc gagcgtgagc tcccggacgt cgaggtgctg
     6721 agcgtcgcct cgccggaggc ggccctggaa gaagtggagc ccggcagcac ggacgtcgtc
     6781 gtgatcgatc ttcgccgtgt gagcggtgag gtccaggaga cgatcacgcg gcttcaggag
     6841 acgcttcagt cgccgatcgt cgtcttcacc gcggacggcg gctcgcgcat gctggccgag
     6901 gcgctgaagg ccggcgtgaa gggctacgtt cgcaaggact caccgcccga ggacctcgtg
     6961 cgcgccatcc aggcggcgcg cagcggcgag ttctacgtgg accccgcgct gtcttcgtcg
     7021 ctgctgctcg aggagggcga gcgcacgctc accgcgcgcc agcgcgagat cctgcagatg
     7081 ctcgcggacg gcctgcacac cgaggaggtc gcgaacaagc tggacctctc gaccgagacg
     7141 gtgcgtacgc acacgaagcg gatcctctcg aagctcggtg cgggcacgcg gacgcaggcc
     7201 gtggcgattg gcattcggtc cgggctgatc gaatagggct cgggcggggg cggtacggcc
     7261 ttggaggagg gtcgttcccc ttcgggcgcc gttcggcttc gcctcagtct tgacgccctt
     7321 cggggaagac cctgcctccc cggcctagtc gggcgtcgtc agtcgagggg aattattcac
     7381 cccaagtgac gacaccctgg cgcggcagcc ggtccccgcc cttactgcta aatcgcgcaa
     7441 agcgggcggc agcaggccca gcgaacgaca cgcaggacgc gcgagccccc gcaagaccta
     7501 gcgcgtctca gcgctcgtct cgtcccccgg cgccggcacg tcggtcgggc tgccgaccgc
     7561 gacacgttcg cccgaggcct tcgcgtggta catcgcggcg tcggcgagct cgagcaggcg
     7621 gtccgggtcc gtgccgtgct gcgggcatga cacgacaccg atcgacacac cgatctggat
     7681 gccgccgtcg ttctcgatct gctcgaccgc cgcgccgatg cggtacgcga ggatcttcgc
     7741 ccccgacgcc gtctggtgcg gggcgagcac gcagaactcg tcgcccccca tgcgcacgac
     7801 ggtgtcgatc gtgcggacgg tcgctcggat ggcggccgcc gcgcccacga gcatgcgatc
     7861 gccggcggcg tggccatgag cgtcgttgat gcgcttcagc ccgttgatgt cgaggagcag
     7921 cacagcgaac gcgtggccgt aacgctgctg cacgccgatg agctgctgca tctgctgctt
     7981 gaggtaccga atgttgtaga ggcccgtgag cgcgtcggtg ttcgcgagcc actcgagctc
     8041 gcgtgagcgt tcgcgcacga gctcgtccgc ggcggccgcc tggatcgcgc cgaaaacggt
     8101 ggcgaggcgc tcggcgatat cgacgaaggc gccgccgtct tcctcacgga gctcgcgccc
     8161 gagcgcgccg atcatcaccg actgcagggc ggcgatgtcg cgcgcaaggt cgtagggcgc
     8221 agggtgctcg cgccccgaga gtgcggcgat ccgctcggcc accgcctgcg accgcgcggt
     8281 gaactcgtcg ccggacgagt tcgatccctc ggcgaccacg gtgagtatgc cggtgatcag
     8341 ctcgggcagc tcccggacga cgcgatcggt ggggattcgc tggatctcat ccagcggcgc
     8401 acgttcgagc acgcgaagga gccaggcttt tgccagctcc gagcgagact cctcgagtcg
     8461 atttagcgat gcgtccagcg cagatgacaa gcggcgaacc ggtggttcag gcacgttcac
     8521 atccctgtcg gccttggcgt cgatgctaac cccgctcata tcgaatccgc aagccatggg
     8581 cggggatatg tcgccccacc catctggtgt agccgtgcac gcccctctcc aaacgcccgt
     8641 acttgcagga tgggcgcgac ccgcggcgaa tggcgtagat cccatggggt ccaccctgcc
     8701 gcgagacgat gcgctggcgg gccgccgcga ggcggtgctc cgccgctggc tcgggatcga
     8761 gatcgcgcga gcgacggtgg aagacctcgc cgccgtcccg atgagcgagc gcctcgtgcg
     8821 cctcgaggat atgtacgacg cggcggtgga agtgctcgcc gcgccggcct cgccgaagga
     8881 gccggctctc cgcgatgcgc tcgatgcgct gctcgcccgg caggagccgt ttgccgtcgt
     8941 ggtcctcgat gccgggcttc gaacgccggc agcccgcgcc gagctcgcgc actccgccga
     9001 cggtgtctgg gcggccgtgg acgggggcga gggccgcacg gccgtactgc tgcccgggat
     9061 cggcccggcc gcggcgccga tcgtcgccga ccggcttcgg gtgcaagcgt ggacctgtgc
     9121 gggcgccgac gggctgctac ccgcgacggg caccgccgtc tacccggacg acggcgtgac
     9181 ggcgtccgac ctcctctcgc tcgcgcacga gcgggtggga agcgaggccg cgggcgatat
     9241 ggatgcggcg accgtcacgc cgatccaccg cgccctttag cccgacccgc cgcactggga
     9301 agatccggcc tgtgccggat ctcctagaca tcgcggaagc acgccggcgg gtgctcgacg
     9361 cttgcttcag gctcgcgccg gaggagctcg agatcggctc ggccctcggg cgcgtgctgg
     9421 cggaagacgt cacgagcgcc gagcggctgc cgccgttcga ctcctcggcg atggacggct
     9481 tcgccctgat cgccgggccc gcgggcgagc tcgagctggc cggcgagtcg cgcgctggcg
     9541 cgccctggag cggcgagctg tcgcacggcc gggcggtgcg catctccacc ggcgcggagg
     9601 taccggccgg cgccgacgcc gtcgtgcccg tcgagcgaac ggaggagcgc gacggccgcg
     9661 tgaccgtgcc cgcgacggca gccggtgaga acgtgcgcta cgccggcgag gacgtgcgcg
     9721 agggcgagac ggtgctccgc gccggtgaga cgctgggccc cgcagagttg gccgtgctcg
     9781 cctcgctcgg acacgcgcga gtgaggtgcg gcgcgcgccc gcgcgtcgcg gtcctcgtca
     9841 caggcgacga gctggtcgcg ccgggcgagc cgctcggccc gggtcagatc cgcgactcga
     9901 atgcgaccgc gctcgcggcc caggccgagc gcgcgggcgc gcgggtcgtc gagcgcgaca
     9961 ccgtgcgaga cgagctcgcg gcgacgacgg ccgccctcgg ccgggcgctc gaagccgccg
    10021 acgtggtgtg catttcgggc ggcgtgtcgg tcggcccgca cgatcacgtg aagcccgcgc
    10081 ttcgcgagct cggtgttgaa gaagtgttct ggggcgtgcg actgaagccg ggcaagccga
    10141 cgtggttcgg cacggccccc ggcaagctcg tgttcggcct gcctggcaac ccggtatcgg
    10201 cgatggtcac cttccacctg ttcgcgcgcc ctgcgctgcg cgcgctgcag ggtggcgacc
    10261 ccagggacgt tcacgtgcgc gctgtgctcg acacggcgat cgagcgcaac ccggcgcgcg
    10321 accaggccgt gcgatgtcgc gtcgacgcgc gcgacgacgg cttccacgct gagccgacga
    10381 aggaccaggg gtcgcacgtg ctcacctcga tgctcggggc cggcgcgctg gcgctcgtgc
    10441 ccgccgggga gggcaggatc gagcacggag agcgtgtgga cgccgaactg ctctgagggc
    10501 gcgtgcggtc cgttacgttt tcaggcgtgc agacgcacga cccgcgtagg gagctcgagc
    10561 gccggcgcac ggctcgcgcc cgggtccacc tcgtccgcgg tccggcctcg acagcgaacg
    10621 aggacggcag cgtcgcgagc cgccaggaag ccgacgtcac tctcccgcgc accgagctcg
    10681 accggctctg gtcgccggag tacctggagc ggctcgcgcg cacgtactgg cgcttcctca
    10741 cgcgcgtctc gctcggtctc ctgcgcgtgc tctacacacc gggctcgcgc gagatcgtgt
    10801 tcctgcggcg gccgttccgg ctgctcacct tccacgcgcc cgaatacaag accgagatcg
    10861 attgcggcat tgtcacgtgg cggatcaacc gcggactgct cgtcgcgccc aacgggcgca
    10921 acaagggtta cctgcgcatc tcggtgagac gcccgccgga gtcgctactg cccgacgatc
    10981 cgacgactac gagcgacgac ttcgcaacca tccgcgtgag ctcagaggtc gagaacttct
    11041 acccgctgct ccgcggctgg ggctggttcg cgcggatcgg ccggtacatc tatgcgctga
    11101 cgcaggtgcg catccacaag atcgtgacga acgcgttcct gcgctcgctg gcgcggctcg
    11161 acctcgcgcc ctccgtcgtc ggtcggctgc gagcgaccga agaccagccg cttgagctcg
    11221 agcccgccga agaggaccgc cagcgcgcgt agacgcgcta gccgaacctc tctcccgtga
    11281 cctcgcggag cttctcgcgg tcgtgccgcg ccatgatgcg gcgcacggtt cccgagcgcg
    11341 atcgcatcac cagcgactcc gtggtcgcgc cgcgagcgcc ctcgtaccgc actccctgca
    11401 tgacgtcgcc gtcggtcacg ccggtcgcgg cgaagaagca gtcgttgccg ctcacgaggc
    11461 ggtcggtgtc gagcacctcg gagaggtcgt aaccctggtc gatcgcggca tggcgctcct
    11521 cgtcgttgcg cggccacagc cgtccaacca tggcaccgcc gatgcacttg atcgcggccg
    11581 cggagatgac gccctcgggc gtcccgccga ttccccagag caggtctacg ggcgagcgct
    11641 cggtcactgc aagcagcgcg gccgacacgt cgccgtcggt gatcaggcgc acgcgcccgc
    11701 cggcctcgcg tacctcgttg atcgcctcct cgtggcgcgg ccgctcgagc atcaccacca
    11761 tcacgtcccg cacgtgcgtg cccttgcgct ccgcgaacag cttcacggtc tcgcccagcg
    11821 gccggtcgag gtcgagcagg tccttgaact cctcgcgggc agccatcttc tccatgtaga
    11881 cgatgggacc cgggctgaac atcgtgcccc gctgcgagag cgcgatcacg gcgagtgcgc
    11941 tcggatagcc cttggccgtg agcgtggtgc cctcgagcgg gtcgaccgcg atgtccacac
    12001 tcggcggcga gccgtcgccg atgtgctcgc cgttgtagag catcggcgcc tcgtccttct
    12061 cgccctcgcc gatcaccacg acgccgtcca tccgcacggt ctcgagcacg tgccgcatgg
    12121 cgtcgacggc ggcctggtcg gctgagatct tgtcgcccac acccacccag cgcgcggcgg
    12181 cgagcgccgc agcctcggtc acgcgcacga gctcgagcgc gaggttgcgg tcgggccgtg
    12241 tcgaccggac cgtcccggct cctgactgct ctccagcgac catctcggac tacaacgtac
    12301 cgggcggttt gcgagtgcgt ccgcccaccg cctggcggat gagaccgccg gctgtgatgc
    12361 ccgccgcgcc gagcaggccg acgaagtagc cccactcgag cgacatgtcc acggtgtcgc
    12421 cgggcctccc aaggatgatc ccgttcagca ggatcagcgc gaacgcgacc atgccgacga
    12481 tcatcgtcac ctcgcctggg cgccaggtga gctggtgccc gcgcacgatg atccaggcga
    12541 ggatgaaggg cgcggcgcag gccgcgagca gcagccagtc gagcacggcg aacgtctgcc
    12601 acgcgttgaa gctcccgtac tcgccgtgga agcgtgagaa ctggttgcac cccggcggca
    12661 cgcccggcct gtcacagctc gtcgagaacc agggcaggaa gagggagagg gcgaggaccc
    12721 cggcgccgac gaagcccacg agctctccga cgttcagtcg ccggacgtcc attgagccga
    12781 aacctacacc gagtcgaggc ccccagcgag cagcggttga gacctgccaa cgtggggcac
    12841 tactgtgacg agagtcgtcc aacgtccggg aaggcttccc cgcatgcgtg cgtctgatct
    12901 gtgtaacggc caatcacgta cggaagacag aggacgttct acctgggtga cggccgccca
    12961 gcctcccatc cccgccaagg accgctagac cagcgtgctc gcccgtcaga agacaacgcc
    13021 ccccggaggg gccgagcccc tgcccaaagg cgactacgcg cggctcttcg aagaggcccg
    13081 ccgtcggacg gccgaccttg tggcgcctgt gtccgaggac gacatggagc gtgtccacaa
    13141 ccctctgatg agccctctcg tctgggacat cgggcacatc gccgcgttcg aagacctttg
    13201 gatctgccag cacctcggca agctcgagcc gctgcatccg gagctcgccg aggtgtacga
    13261 cgcgaccgag acgccacgca aggaccgcgg caccctgccc ttcctccgct tgagcgacgc
    13321 ctgggattac atggccgagg tccgggctcg gactctgtcg gtgctcgacc gctacgaggg
    13381 cgattcgttc atctgggaga tggtcctcga gcacgagcac cagcacaacg agacgatgct
    13441 gcaggcgctc cagctcgctc cggctggcgt gtacttcccg ccctcgcatc gcctggccgc
    13501 ttcgccggat gaggcggccg agcctcccga gcgcgtgttg atcgaggccg ggccattcct
    13561 gatgggcgat ccgggcgtcg gcttcgcgta cgacaacgag cggccgcagc acgaggtgac
    13621 gctgcgcgcg ttcgagatcg atcggtcgcc ggtgacggtc ggcgccttcc gcgagttcgt
    13681 cgaggagggc ggctacgagc gccgcgattg gtggtcggac gagggctggc tctggcgcga
    13741 ggccgaaggc gtcgagcggc cgctctactg gaccgaggac ggcggccagc gcttcttcga
    13801 tgccgtcgaa ccgctcgacc ccgcgcttcc ggtgatgcac gtgtcgttcc acgaagcgca
    13861 ggcctatgcc gagtatcgcg gccgccgcct gccgagcgag cccgagtggg agaaggccgc
    13921 cgcgtgggat cctcgccgcg gcgagcacag gcgccacccg tggggcgaca gtgagcccga
    13981 ctggggccgc gcgaacctcg accagttctc gttccgcccc gacgccgtcg gcgcgcacgc
    14041 ggccggcgac tcggcttacg gcgtgtcggg cctcgagggc gactgctggg agtggactgc
    14101 cagcgagttc cacggctacc cgggcttccg cgcctatccc tacagcgagt actccgagat
    14161 cttcttcggc ggcccctacc gcgtgctgcg cggcggctcc tgggcgacgc gcccgcgcgt
    14221 cgtgcgcacg acgttccgca actgggatca cccgcagcgg cgccagatct tcagcggctt
    14281 ccgctgcgcc aacgacgtgg agtcatgagc ggcgcgacga tccggatcga ctcccacatg
    14341 gaagcgagcg ggtcgctcgg gtcgatggcg gacgacgtgc gcgccggact gagcggtgcg
    14401 ctcatggagc tctcgccgaa gtacttctac gacgaccgcg gatcggagct gttcgagcag
    14461 atcacgtcgc tgcccgagta ctaccccacc cgctgcgagc gcgagatcct caaccggcgg
    14521 gcgcccgaga tcgtggagct gtgcgacccg ctcgagctgg tggagctcgg ctcgggcgtc
    14581 gcctcgaaaa cgcgcgcgct gctctacgcg atggccggcg ccgacaagct ccgccgctac
    14641 gtgcccgtgg acgtgtcgga agccgtcgtc acacgctgcg ccgaagagct gaccgaggcc
    14701 taccccggcc tcgaggtgca cggcgtggtc ggcgacttcc tccagcacct cgaccggctc
    14761 cccgacggcg agcgccgcat ggtggcgttc ctcggctcga cgatcggcaa cctccacccg
    14821 gcggaccgga cggtgttcct cgcggcagtc cgcgacctgc tcggcccggg cgaccggctc
    14881 gtgcttggcc tcgacctcgt gaaggatgtg cgcgtgctcg aggctgccta caacgacagc
    14941 gccggggtga cggccgagtt caacctcaac atgctgcacg cgatcaacgc cgcgctcggc
    15001 gcggacttcg agcctgcggc gttcgagcac gtggcgttct tcgagcccga acactcgtgg
    15061 gtggagatgc gcctccgcgc gagtgaagac cagcgcatca cgatccccgg cgccgccttg
    15121 gagctcgacc tcgaggcggg cgacgagatc cgcacggaga tcagcacgaa gttcacgcgc
    15181 gaggggatca cggccgagct cgctgacgcc ggcatggcga tgcgttcgtt cttcacggac
    15241 gagcgcggct ggttcgggct agcggtcgcc gcgcgggttt gagcacgagc gcgaacctaa
    15301 agcgagccgc ggttgtaggg tgccgctccg atgaacctcg acggactcgg cactctcgtc
    15361 gcgggcggcg catcgggcct cggcgcggcg accgcccgcg cgctcgcgca gcgcggcgcc
    15421 aaggtcgcga tcgcagacct cgacgaggac aagggcgcag cgctcgccaa ggagatcggc
    15481 ggtatctcgt tccgcgctga cgtgaccgac gaggcggagg tcgagggcgc cgtggagggc
    15541 gccgtccagg agttcggctc gatcggcttc gcggtctcgt gcgcgggtat cggctgggcg
    15601 gaacgggtcg tgggcaagga cggaccggcg aagctgcgcc cgttcgagac agtggtgcgc
    15661 gtgaacctga tcggcacctt caacgtgctg cggctcgcgg ccgctgcgat ggcgcagaac
    15721 gatccgggcg aggacggcga gctcggcgcg gtcgtcatga ccgcctcgat cgcggcgttc
    15781 gacggacaga tcggtcagag cgcgtatgcg tcgtcgaagg gcggcatcgt cggcctcacg
    15841 ctgcctgccg cgcgcgacct ctcgcgcaac ggcatccgcg tctgcacgat tgccccgggc
    15901 acgtttgaca cgccgctgct cgcgggcctg ccggaggcgg cgcgcgaggc gctcggccag
    15961 cagatcccct tcccgcagcg gctcggcaag cccgaggaat tcgcagcact cgcctgccac
    16021 atcgccgaga accgcatgct gaacggcgag gtgatccgcc tcgacggcgc ccttcgcatg
    16081 cctcctcgct gagcgggtaa ggaggccgcg tgcgtgcagc gctcgtagag gagatcggca
    16141 aacggcccgt cgtcaccgag acggcgccgc cgaagcaagg acccggcgag acgctcgtgg
    16201 cggtcaccgc tgcgccgatc aacccgatcg acctgagcac ggcagccggc cgttaccctg
    16261 gcggcagccc ggagccgcct tacatccctg gacgtgaggc cgtgggcacc gtcgccggct
    16321 cggaccgctt cggcgagggc acgcgtgtct acgtgggcgc gcctctcgcc cgcaacggcg
    16381 ccatggctga gttctgcgcg tgcccggaca gcgaggtctt cgccgttccc gccggcgtcg
    16441 acgactcact cgccgcttgc ttcggcgtgg cggggctcgc gggctggctt cctgttacgt
    16501 ggcgcggccg gctcgagaag ggagagaccg tccttgtgct cggggcgacg gggccggttg
    16561 gcagggtggc tgtgcagtgc gcgaagctca acggtgcagg ccgcgtgatc gccgcggccc
    16621 gcgacccggt ggagctggag cgcgtcaagg agctcggggc cgacgccacc gtgcggatcg
    16681 gcgatgtgga cgacctggtg caggctttcc gggacgccgc cgagggcgac atccagctcg
    16741 tggtcgaccc gctgtggggc cagccggccg cggccgcgct cgaagcgctc gcgctcttcg
    16801 gccggcacgt tcaggtcggc cagacggcgt ctccgaccgc cgagattcgc tccggcgcga
    16861 tccgcacgaa gaacgtcgag atcctcggcc acaccagcgg gctcgcgccg gtcgaggtgc
    16921 gcgagcgcgc ctaccgggag atgtgcgagc acgcagcggc gggtcgtttg acgctcgact
    16981 acgaggagat ccccctcgag caggtgaggg aggcctggga gcgccaggag cacttcccgc
    17041 acaagaagct cgtcctccgt ccgtgaccga tctcgccctg ccccggggtt cccgcgggga
    17101 gatgtaccgt aggatttctc actccctcga ccgccgaacc aatcttcagg aggccagtag
    17161 ccaatgccgg aagcagtgat agtcgacgcc gttcgaacgc ccatcgggcg ggccttcaag
    17221 ggctccctca ccggggtccg ccccgacgac ctcggcgcgt acgtcgtcga ccagctcctc
    17281 gagcgcaacc ccgaggtgaa gcccgaggac gtggaggatt tgatcgccgg gtgcggcctt
    17341 ccgcagggcg agcagtcgtt caacatcggc cgcatcatca cgctgctctc gcgcctgccg
    17401 aacagcacca ccggcacgac cgtgcaccgc tactgcgcct cgagcctgca ggccatccgc
    17461 atcgccgcgc acgccgtgat ggcgggcgag ggcgacacct tcatcgcggc cggtgtcgag
    17521 cacgtgagcc gcgtcggcat gtggagcgag gacgggctgc cccagctgat gggcgccctt
    17581 gcgcagaacg cccctgaggg cgtcgacgtc ccgcaggcca acttcaagaa cgacaagatc
    17641 aacggcgagg acgggcgcac ggacgcgtac atcgccatgg gcatgaccgc cgagaacgtc
    17701 gcggagaagt acgacgtgag ccgcgacgac atggaccgct acgcccagcg ctcacaggag
    17761 cgcgcggtga cggcgcagca ggacggcttc ttcgagcgcg agatcgtcgc ggtcgagctg
    17821 ccggacggca atacggtcgc gaaggacgat ggcccgcgtg ccgactcgac gtacgagaag
    17881 ctgcagacgc tcaagcccgc gttcaaggag gacggccgcg tgacggccgg caactcctgt
    17941 ccgctgaacg acggcgccgc tgcggttctc gtgatggagc gcagccgcgc ggagggtctt
    18001 ggcctcaagc cgcgcgcccg tatcgtcgcc tcagcgacga gcgggctcga gccggagatc
    18061 atgggcgtgg cgcccatcgg cgcgatcaag aacgtgctcg atcgcgcgag catgacgatg
    18121 aacgacgtgg acatcgtcga gctgaacgag gcgttcgccg cacaggtgct gccgatcgcc
    18181 gatgagtgcg ggatcgacct cgacaagctg aatccgcacg gcggcgcgat cgcgctcggc
    18241 cacccgttcg gcatgaccgg cgcgcgcatc atgaccacgc tcctcaacgg gctcgagacc
    18301 gacgacaaac agatcggcct cgagaccatg tgcgtcgcag gcggccaggg catggcaatg
    18361 cttatcgaac ggctcaatta gccggcgctt cctgatcctc cacgggtggc agggctccgg
    18421 ccctgaccac tggcagacgt ggctcgccgg gcgccttcgg gcgctcggcg agcatgtctc
    18481 ctatccgcag ctgccaaacc cggaccggcc cgtgctcgcg gagtggcgca cggcgctcgc
    18541 ggcggagctc gacgcggcgc gcgatcgcga gctggtcgtg ctttgccact ctctcggcgg
    18601 catcctctgg ctgcaccacg ccgctcggcc cgtgtctgac gtcgtgccgg agcgtgtgct
    18661 gctcgtcgcg ccgccctgcg cgccctggat cgcacctgag ctcgccgcct tcccctacgc
    18721 gccaatggac cgcgcgggcg tcgcgcgggc agcccgaacg acccgcatgg tctgctccga
    18781 caacgacgag tactgccccc gcggcgcggc gaacgtgtat cccccggtgc tcgggatcga
    18841 ctgcgaggtc ctcccgggcg cggggcacat caacaccgat gccggctacg gcgcctggcc
    18901 gctcgccgag cggttcgccc tcagcgacgc ggtgtgagac gcgcgctcaa cgaatcgacc
    18961 taaggtacgc gacgaccttc gccctgtcgg ccgggttccc aagccggtcg gggagcgt
//
LOCUS       DASVXS010000159        18986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1316116, whole genome shotgun sequence.
ACCESSION   DASVXS010000159 DASVXS010000000
VERSION     DASVXS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18986
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316116"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VF752_08810"
     CDS             <1..667
                     /locus_tag="VF752_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8855690.1"
                     /translation="FMWGANVLIYNADKIKENLDSWNVLYDPKYKGKITVPDNPIQIA
                     DVALQFEGAQDPYAIDQSTLDKAKSKLDQQRPLVRKYWVLATDFEQLFKSGDAVVGAG
                     WPLMTQDLKKAGMHVGEVLPKEGVTGWSDSWMISNKAKHPICAYKWMNYVTSPKVQAQ
                     VADVTGYSPPNLKTCEVVGSQRCKDLHIRDTKYYDTIKYWQTPTSPTNYRQWTDTWAQ
                     VRG"
     gene            634..1575
                     /locus_tag="VF752_08815"
     CDS             634..1575
                     /locus_tag="VF752_08815"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855691.1"
                     /translation="MDGYLGASERLSLEAPRRIVPRRLTRFLYAHPRGRLGLLLGPAV
                     LWLLIFYLVALFLLLLTSLWQQDVFTAELVHKWTFSNYTKVFSGQDGLWLRVFGRTIL
                     LASIVTLIDIALAIPLAWFMARVAPPRWRTLLFLAVVVPLWSSILVRIFAWRTILGGQ
                     GVLNNFLVDVGILNHPSATFLYNEKAIALTWAYVWLPFMVLPIFTALEKIPDSFIEAS
                     RDLGASPWTTFKRVVFPLAVPGVVAGSIFVFSLTMGDFVAPQLVGGGSQVLGGAIKDR
                     FGVAADYPFGAALAMLTLLTLTVFLTITRRSGAMENL"
     gene            1572..2381
                     /locus_tag="VF752_08820"
     CDS             1572..2381
                     /locus_tag="VF752_08820"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855692.1"
                     /translation="MTSTRSKWIFGGTAALVFLFLYLPLALIVLYAFNKTNVNAWPFP
                     GFSTHWFEKLWHDPEPKNAAWLSVKIATLSTIFALVIGTGMAFAFARFRFFGKEGLNF
                     AVTLPIILPGVITGVALASFFLFTGTDLSMRTVVIGHTTFCIVLVFNNVLARLRRLPL
                     SYSEASRDLGASGFQTFRFVTLPSIRTALVAGALLSFALSFDEIAVTFFLAGADTTTL
                     PLWILGAFRNSRSLPEVNAVAAIILLVSLPMIAVAAYLMRDEGAGSRIVEK"
     gene            complement(2505..2906)
                     /locus_tag="VF752_08825"
     CDS             complement(2505..2906)
                     /locus_tag="VF752_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855693.1"
                     /translation="MVLVLVLVARVLEHLLLLARASHCVQRTLRERGGAVGHLLNRRG
                     SLLGDIGTGDIHRLGFSAECLDAPLQLARVALRLAQVLLEALLVRRARCHRDVRLQRR
                     LELLLFPVRLVEVLNQLRVSGIHFRHTTVPS"
     gene            complement(2915..3352)
                     /locus_tag="VF752_08830"
     CDS             complement(2915..3352)
                     /locus_tag="VF752_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855694.1"
                     /translation="MPELDEERPFDDERLPDERDEPERLPEERDEPERLEDARVEPER
                     LDDERLLEPDRARVLDPDRERDDDERLDAVARLREVERRRPPEAARRSAAGISEVATD
                     FVSVGIRRWRKFAIFSSSRLMRFASWAVSRSPTDVASVSIAVY"
     gene            complement(3418..3792)
                     /locus_tag="VF752_08835"
     CDS             complement(3418..3792)
                     /locus_tag="VF752_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855695.1"
                     /translation="MTIWDWVLFLHLLAMAFFVGGQLMLAAIVVPVVRGTGDRDALRA
                     VARRFGVGTLLAIGVLVATGAAMASHFDLRDDGKLQAKLALVVVAGLLIGWHMRRPQQ
                     HAIEGIVFLVSLAIVALGVSLP"
     gene            complement(3818..4231)
                     /locus_tag="VF752_08840"
     CDS             complement(3818..4231)
                     /locus_tag="VF752_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8855696.1"
                     /translation="MKPLAQLDVERVQDALVVHLEGEIDISNAAELGAALEAAATSDT
                     LGIVLDLSHTSYIDSAGVHLLFRLGGRLTRRRQQLRIVVPAESPIKKVIKLTGVGWTV
                     PHDDSVDEAVRGLRAEVRPVPGSDSWNSDPNAEWW"
     gene            complement(4228..6450)
                     /locus_tag="VF752_08845"
     CDS             complement(4228..6450)
                     /locus_tag="VF752_08845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8855697.1"
                     /translation="MSAERKFPLPSFGAIRDLLRRSEQAYDGADLLTARKFVGVLWAV
                     SGTIALGLLLVEPPTESPIGAAGWLAGVAVVGASFLGARWVLGDAEEPGFDGLLIATY
                     AGVTQIAALEWLAGGDSAYRALFVLWLGAGVGVHPPRRGVPFIGAVVAAGCMPLLYAG
                     HETRTLIDTVTNAVLWVAVGFVLLALISRVRSQRREMHELERDALERAEQAVRRVQGL
                     EAVSDAALAHLPFDDLLSELLGRITKVLQVERAAILLNDGDTGALRVRAAHGRDAEQL
                     KRRRVPLGEGLAGRVAAEKRPVFIRAMEDASEASPLVPGADVSSLLGVPLLVRGGTVI
                     GVLQVASSDDAGFSEDDSRLLQLAADRVAVAIDRAQLNEQAHRIAETLQRSLLPSRIP
                     DIPGLTLATRYQPGAAGSAVGGDLFDVVEYLDGRIGLAIGDVVGRGIEAASLMGQLRN
                     ALRAYAIEGGEPADVVERLNRVVGHWDEGQIATLLYVLVDPRTSTLTYSTAGHLPPLV
                     RGPDGTTSFLPTGEYVPLGVLPFGAYRESVVAIEPGSTLILYTDGLVEERGMSVEDGM
                     ERLSHAVAGGPEDPDALCDHVLETVPSDGIAGDDVAVLAARLDPVPSNKFELRLPAEP
                     ESMVAVRRTLERWLDAHGAERQVMYELTVACGEACMNVVEHAYPGGDAVFVLEATHEN
                     GEVELVIRDFGYWRPPRKSDRGRGLELMRRLTDSAKITPGPQGTTVRLRRGLKTKVTA
                     "
     gene            complement(6533..6940)
                     /locus_tag="VF752_08850"
     CDS             complement(6533..6940)
                     /locus_tag="VF752_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855698.1"
                     /translation="MPARTRELPPSEDAAIAIALAETALPFADSPSAEVEHWIRILRM
                     HGEVGSALQALGAGERPLSELDAGADEAEGVRVHSVYSEASRIAGEHGDEIVSTADLL
                     SALIELYGPLFDDVLRAHGVATAELLERLGRVP"
     gene            complement(7018..7428)
                     /locus_tag="VF752_08855"
     CDS             complement(7018..7428)
                     /locus_tag="VF752_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855699.1"
                     /translation="MGADDAIQELVDLEYAWLEATRTRDMEFLERLLGEEFTLTTGRP
                     GVEVRSRAEWLDVTRDSYAIDSFEFADVDARVYGDAALVRSHYRQRGRMGGEDRTTAY
                     LMSDVLVRRDGRWQAVARHISPLEQDPPDRGLRG"
     gene            7558..8790
                     /locus_tag="VF752_08860"
     CDS             7558..8790
                     /locus_tag="VF752_08860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8855700.1"
                     /translation="MSANVARRAVLGLLGRLKRGRVELVDAGRRLEFGDGDGPAATVT
                     VRSPAFYRQVLRGSLGIGDSYTRGLWDCDDLVALVRVLARNMETIDRVRRRMAPVLSP
                     VQRVASRSWLPTPARSRRQIAAHYDLGNDFFELFLDETMTYSCAVFDTPGADLADASR
                     AKLERVCAKLDVHPGDHVLEIGTGWGSFALHAATTRGCRVTTTTLSKEQHDYARERIE
                     DAGLRDRITLLLDDYRHLRGRFDKLVSIEMIEAVGWQNFGTFFRRCSELLKSTGAMAL
                     QAITIDDRAYELEKAKRTFIKSRIFPGGALPSVEVMTREVARSTDMRAVHLEDITPHY
                     PPTLAAWRRRLLENAGRVLARGYPERLVRQFELYFAYCEAGFTERRIGDVQMVLAKPA
                     FRGEQAVLEGARHAVLAT"
     gene            8787..9164
                     /locus_tag="VF752_08865"
     CDS             8787..9164
                     /locus_tag="VF752_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855701.1"
                     /translation="MNGLKVATAVFGSARAAIGAGLAVDPERLGRRWIGKDARRPRTQ
                     VALRGLGARDVAIGGGTVAAALTEDDVLLWLCGGIVSDVADIAATLSERENVPRRGVR
                     LTAVLAGVGVIAGAALAGAWARG"
     gene            complement(9161..10606)
                     /locus_tag="VF752_08870"
     CDS             complement(9161..10606)
                     /locus_tag="VF752_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8855702.1"
                     /translation="MALAPPPQISMNPLKRLRMDLERERRRTEPLPPGELQFSIARTR
                     RMRTDPLRLVLECYERYGPVFSMRLLHEPVVFVIGPEANHYVTVSHAQNFRWRDGSFG
                     DLIPLLGDGLLTIDGDYHRRSRKLMLPVFHRDRIAATAGTMVEEAERALEGWQPGRQV
                     DMYHWTRALAMRIAMRALFGFNPDDKSHGFDEAAEFEEALSYYATDYAIQILRGPRTP
                     WARMIKARERLDRLIFGQISRRRTSGDAGDDILSLLLEASDEDGNTLSDRAVRDQVMT
                     LLFAGHDTTTSTVTFLFYELARHPQALARLIEEQDRVLGGRMATAAELMGGEALPELE
                     MAVDETLRLYPPAWVGPRMSVEPFELCGVRVPAETYVNYSSWVTHHLPEVYPDPEAFV
                     PERFAPEAKAELPKGAYVPFGGGSRTCIGMRFGQLEIKTIATLILQRCRLELQPGYRM
                     RIRQMPTLSPIDGLPMRVLERTQPAALARAA"
     gene            10697..12520
                     /locus_tag="VF752_08875"
     CDS             10697..12520
                     /locus_tag="VF752_08875"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX8855703.1"
                     /translation="MGSRRLFVLACLLALLVVPPAAHSAPPPSVADYYLDADVGSDLE
                     DCLGPKGAPDGHAPCATFDRIEQLNPSSVVTIHLAPGEYDAPSSAPSQFMYIVGDTDA
                     TITSGLEFEHGASLSNVTVTSADDPGAPALELRNADTSPADYLLDGVTAVASSTGGPS
                     ANAVEVTGPVDLAATDSVFAVGDSGWVVHSDGADVSLQLNGTSLQVGAGGRGLQIDGG
                     GEAALTDSTVDGPGAAYGIVVNGLSAALTLERSSIAAGSTAVSVRHHFGSGTTTANVR
                     DSVLQSMSASTAPDDGALVASTQLSPADQSPHVAVTVTGSTLVNMADADPDHGPDGAA
                     IAYQSGGTATIALHNTLVYAPHLPDLTAIQSGIYPAGSAAITADHSIFGTPTSFEGAF
                     AADNGADGNSGADPLLADPEDGDFTLLAGSPAIDAGDASAVTGGESDVNGAPRSQDGD
                     GDCVSLPDVGAFERSATLTCTPKSTPPGEADHATGKPTDTTKPKAHASLRRALKLRTA
                     LRRGVRTRVYSNEPADVNLTAELGPRSAHKLGLAAKPVVVARAKTALNGNAARVVTLK
                     FTRKAKSKLRGGKTIKLAIRISVRDVAGNTSTLVRHITLKR"
     gene            complement(12522..13583)
                     /locus_tag="VF752_08880"
     CDS             complement(12522..13583)
                     /locus_tag="VF752_08880"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8855704.1"
                     /translation="MGAVLAGQGLERIAEIAERHAGAPVAVIVPQLGAPPAWAEYERY
                     VAGRLAGEAVERPAGVVNEVPIATGGREVGSVLLLGQGRPDAGEYAQVAALAALTSLA
                     VSEAHAEAEQSLRGSLIEQLLENDDVAAEDVVRRGRRMGIDLSSGGVGICADPGERTP
                     GRLLAVISAERADAVADVVRGRVFALVPGSIEEAQALAARLGRQATVGVSSRYSSPGA
                     LRRALEEAELVLEVRSAGVEPRDGEMDGGAYRLLFRVLASHPEEVRSFYEDTVAPLVR
                     YDETYSSDLTGTLSAYLDSNCSIKATSAAVHAHRHTVGYRLERVRELTGLDPFRSEDR
                     ERLGLGLKAYRIVAPLLPR"
     gene            complement(13637..14005)
                     /locus_tag="VF752_08885"
     CDS             complement(13637..14005)
                     /locus_tag="VF752_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8855705.1"
                     /translation="MKMVVAYIRHEAFEPIRQELLDAGFPSLSITEVKGSGRQKGITE
                     QYRGATLAIHLRPKLKIECVVETTDVEQVIGTILRHARTGDVGDGKIFLLPVEDAIRI
                     RTGESGEEVLQAHESEEIPA"
     gene            complement(14002..15492)
                     /locus_tag="VF752_08890"
     CDS             complement(14002..15492)
                     /locus_tag="VF752_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8855706.1"
                     /translation="MRPTLRRRALGAFALSIAAVLAVPAGAFAADGPSLTETVSSVNT
                     TWVIVAGILVMFMQAGFALLEIGFSRMKNAGAGVAKILMNFSIASIAYWAVGFALAFG
                     GSGAIAGTHGWFLNVGHTAAAAAKDIPLLGTYDISPGALLFFQFVFCAVSLAIVWGTT
                     LERIKFSAYVIYAVIFSALIYPILSHWIFGGGWLQVSVGMQDFAGSTVVHLIGATGAL
                     AALLLLGPRRGKYDENGKPRAIPGHSMPLVGLGILILWLGWFGFNPGSTLGAIGNRFA
                     EVVVVTNLAAAAGVIAAAATIHLLRKTVDIGMVGNGAIAALVAITAPSGYVEYWAAPI
                     IGAVAGVIVVVGVLAIDKVLDDPVGALSAHGLAGIWGTLSCGLFTSPRLATLNAVGDP
                     GLVYSGSFHQLGAQALGVAVAFACVFSVSYVVFWTIKKTIGLRVTPEQEEAGLDIAEH
                     GMYGYPEQFIPAPEIGAASSGPLAVPPQPAPVPSPALMTTRDGATA"
     gene            complement(15662..16087)
                     /locus_tag="VF752_08895"
     CDS             complement(15662..16087)
                     /locus_tag="VF752_08895"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX8855707.1"
                     /translation="MPARQEDIPSTIERSPKKVRDTYEETLNSAHEQYEGDEERAHRV
                     AWAAVKHIAEKKGDHWELKDEPGPSDEQAARGGQAARDNPKPTAGGVDATKSKDELYQ
                     EAKEAGIEGRSDMSKEELVDALEKYNERETRRARERSRS"
     gene            16406..16786
                     /locus_tag="VF752_08900"
     CDS             16406..16786
                     /locus_tag="VF752_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8855708.1"
                     /translation="MSDDVLIYSDRPLYVRLPEREAEMLDRAAREGRTSKRQLVTSLV
                     QRFLGEEGPLVVDGRGVAETGRHDFSAGSAPEVLTLEQAAELLMVAPEAIEELAAKGE
                     LPGRRIGGEWRFSRSALVDWLAAR"
     gene            complement(16783..17436)
                     /locus_tag="VF752_08905"
     CDS             complement(16783..17436)
                     /locus_tag="VF752_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8855709.1"
                     /translation="MSRAPASMLARGPWPPESVMVHWREDQYEPPPEVDALADEAVRA
                     LSERGSPAHDGLAARLAGFGGNDRTLALELQPARWALRLVDHEAAAGSMTALTVVRDG
                     DGRWLAGRRADWLASWAGRWALGAGGAVEVEENPADTLSRELVEEWQLTPERMSVEAL
                     VQSPTGLAMLVGLAHVAAGADAVPDHEHDEFAWWPADPAAWPAEADERLRRVGHLLA"
     gene            complement(17433..18257)
                     /locus_tag="VF752_08910"
     CDS             complement(17433..18257)
                     /locus_tag="VF752_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8855710.1"
                     /translation="MRPTEEQTILVTGATDGLGRQVALDLAGRGATVLVHGRNPEKIE
                     DTVDEIRRGSGKAESYVADLSSLAEVRRLAEDVRADHDRLDVLVNNAGIGSGSDGTRR
                     ELSADGLELRFAVNYLAGFLLTIELLALLRASAPARVVNVSSAGQMPIDFADPMLEHG
                     YSGVRAYCQSKLAQIMFTAELAERVPPDELAVTALHPATYMDTNMVRESGAPITSTVR
                     QGADATERLIADPSLDGVSGRYFNGMRETRPDPQAADPAARTQLWDLSERLVAGAA"
     gene            18361..>18986
                     /locus_tag="VF752_08915"
     CDS             18361..>18986
                     /locus_tag="VF752_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8855711.1"
                     /translation="MNELRTEDRRLASDAVHERLRAEILDGRLAPGDPLPSERTLSAE
                     HGVNRHAVREAVKRLQQAGLVRVSHGGATRVLDWRATGGLDLLADLSMSGQGHDLLRS
                     IAEMRASIGADAARLCARRAPADTRERLTHLAAPRKDASYEEQLVAYEELWQLIVDGS
                     GNLGYRLAFNSLVGARHGHGVNSRIYADEVGDVRAWRALAAAVTAGDEP"
BASE COUNT         2919 a         6777 c         6475 g         2815 t
ORIGIN      
        1 tttcatgtgg ggggccaacg tcctcatcta caacgccgac aagatcaagg agaacctgga
       61 ctcctggaac gtgctctacg acccgaagta caagggcaag atcacggtcc ccgacaaccc
      121 gatccagatc gccgacgtgg cgctgcaatt cgagggagcg caggacccgt acgcgatcga
      181 ccagagcacg ctcgacaagg ccaagtcgaa gctggaccag cagcgcccgc tcgtgcgcaa
      241 gtactgggtg ctcgcgaccg acttcgagca gctgttcaag tcgggcgacg cggtcgtcgg
      301 tgctggctgg ccgctgatga cgcaggacct caagaaggcc ggcatgcacg tcggtgaggt
      361 gctgcctaag gagggagtga ccggctggtc ggactcctgg atgatcagca acaaggccaa
      421 gcacccgatc tgcgcctaca agtggatgaa ctacgtcacc tcaccgaagg tgcaggcgca
      481 ggtggcggat gtgacgggct acagcccgcc gaacttgaag acctgtgagg tcgtgggctc
      541 gcagcggtgc aaggacctgc acatccgcga caccaagtac tacgacacga tcaagtactg
      601 gcagacgccc acctcgccga ccaactaccg gcagtggacg gatacctggg cgcaagtgag
      661 aggctgagcc tcgaggcgcc gcggcgcatc gtgccgcggc gcctcacgcg cttcctctac
      721 gcgcatccgc gcggccggct cgggctgttg ctcgggccgg ccgtgctctg gctgctcatc
      781 ttctacctgg tggcgctgtt cctgctgctg ctcacgtcgc tgtggcagca ggacgtgttc
      841 accgccgagc tcgtccacaa gtggacgttc tcgaactaca cgaaggtctt cagcggccag
      901 gatggcctct ggctgcgcgt cttcgggcgc acgatcctgc tcgcgtcgat cgtcacgctg
      961 atcgacatcg cgctcgcgat cccgctcgcg tggttcatgg cgcgcgtcgc gccgccgcgc
     1021 tggcgcacgc tgctgttcct cgcggttgtg gtgccgctct ggtcgagcat cctcgtgcgg
     1081 atcttcgcct ggcgcacgat cctcggcggg cagggcgtgc tcaacaactt cctggtggac
     1141 gtcgggatcc tcaaccaccc ctctgcgacg ttcctctaca acgagaaggc gatcgcgctc
     1201 acgtgggcct acgtgtggct cccgttcatg gtgctgccga tcttcacggc gctcgagaag
     1261 atccccgact ccttcatcga agcctcacgc gacctgggcg cgagcccgtg gaccacgttc
     1321 aagcgcgtgg tcttcccgct cgccgtgccg ggcgtcgtcg ccggctcgat cttcgtcttc
     1381 tccctcacga tgggcgactt cgtcgcaccg cagctcgtcg gtggcggttc gcaggtgctc
     1441 ggcggcgcga tcaaggatcg cttcggcgtg gccgcggact acccgttcgg cgctgcactc
     1501 gcgatgctca cgctgctcac gctcaccgtg ttcctcacga tcacgcggcg cagcggcgcc
     1561 atggagaacc tgtgaccagc acgcgctcga agtggatctt cggcggcacg gcggcgctcg
     1621 tgttcctgtt cctgtacctg ccgctcgctc tgatcgtgct ctacgcgttc aacaagacga
     1681 acgtgaacgc gtggccgttc ccgggattct ccacgcactg gttcgagaag ctctggcacg
     1741 acccggagcc caaaaacgcg gcctggctct cggtgaagat cgcaacgctg tcgaccatct
     1801 tcgcgctggt gatcggcacc ggcatggcgt tcgcattcgc ccgcttccgt ttcttcggca
     1861 aggaggggct gaacttcgcg gtcacgctcc caatcatcct gcccggcgtg atcaccggcg
     1921 tggcgctcgc ctcgttcttc ctgttcacgg ggaccgacct ctcgatgcgc acggtggtga
     1981 tcggccacac caccttctgc atcgtgctcg tgttcaacaa cgtgctcgcg cgattgcggc
     2041 ggctgccgct gtcctactcc gaggcctcgc gcgacctggg cgcgagcggc ttccagactt
     2101 tccgcttcgt cacgttaccg tcgatccgca ccgcgctcgt ggccggcgcg ctgctgtcgt
     2161 tcgcgctgtc attcgacgag atcgcggtca cgttcttcct cgcgggcgcc gacacgacca
     2221 cgctgccgct gtggatcctc ggcgcgttcc gcaacagccg ctcgctgcca gaggtgaacg
     2281 cggtcgccgc gatcatcctg ctcgtgtcgc tcccgatgat cgccgtcgct gcgtacctca
     2341 tgcgcgacga gggcgcaggc tcacggatcg tcgagaagta gctcgcgacg cgtcgtccaa
     2401 acgcgactcg tgtgcagcta cgcgaaggcg tgcgcgttga aggatgaaaa cgcgcgcaat
     2461 tcgggtaagg gccttgggaa ctccgagcga cccggaaaca agcactagga ggggaccgtc
     2521 gtatggcgga agtgaatacc cgagacgcga agttgattca gtacctcaac gaggcgtacg
     2581 ggaaagagaa gcagctcgag acggcgctgc aggcgcacat cgcgatgaca acgcgcgcgc
     2641 cgtacaagaa gcgcctccag gagcacctgc gcgagacgaa gggccacgcg cgcgagttgc
     2701 agcggcgcat caaggcactc ggcggaaaag ccgagacggt ggatatcccc ggtcccgatg
     2761 tcgccgagaa ggctgccgcg gcggttcagg aggtggccca ccgcgccgcc gcgctcgcgc
     2821 agggtccgct gcacgcagtg cgaggcacga gcgagcagga gaagatgctc aagaacgcgc
     2881 gcgacgagta ccaggacgag caccacgaga tcgctcagta cacggcgatc gagacgctcg
     2941 cgacatcggt gggcgaccgg gaaacggccc agcttgcgaa gcgcatcagg cgcgaggagg
     3001 agaagatggc gaacttcctc cagcgcctga tcccaacgct cacgaagtcg gtcgcgacct
     3061 ccgagatccc ggcggccgag cgccgggcgg cttcgggtgg ccgccggcgc tcgacctcgc
     3121 gcagccgcgc cactgcgtcg agccgctcgt cgtcgtcgcg ctcgcggtcg gggtcgagca
     3181 ctcgtgcgcg gtccggctcg agcagccgct cgtcgtcgag ccgctccggc tccacgcggg
     3241 cgtcctcgag ccgctcgggc tcgtcgcgtt cctcgggcag ccgctccggc tcgtcccgct
     3301 cgtcgggcag ccgctcgtcg tcgaacgggc gctcttcgtc gagctcgggc acgcgctcgc
     3361 gcgcgcggag ctcgagccgc agctagctca cagccgtcgc tcggcgtgtc gcggccgtca
     3421 gggcagcgac acgccgagcg cgactatcgc cagcgacacg aggaacacga tcccctcgat
     3481 cgcgtgctgc tgcggccgtc gcatgtgcca gccgatgagc aggccggcga ccactacgag
     3541 cgcgagcttc gcctggagct tgccgtcgtc cctcagatcg aagtgcgatg ccatcgcggc
     3601 gccggtcgcg accaagaccc caatcgccag gagcgttccg actccgaacc gacgcgcgac
     3661 cgcgcgcagg gcgtcgcggt ccccggtccc gcgcacgacg ggcacgacga tggccgcgag
     3721 catcaactgc ccgccaacga agaaggccat ggcgagcagg tgcaggaaga ggacccagtc
     3781 ccagatcgtc acgcccggca gactgccgga agcgggatta ccaccactcc gcgtttggat
     3841 ccgagttcca gctgtcggac ccgggcaccg ggcgcacttc cgcgcgcagg ccgcgcacgg
     3901 cctcgtcgac ggagtcgtca tgcggcacgg tccagccgac accggtgagc ttgatcacct
     3961 tcttgatcgg tgattccgcc ggcaccacga tccgcaactg ctggcgccgc cgcgtgagcc
     4021 gaccgccgag gcggaacagc aggtgaactc ccgcgctgtc gatgtaactc gtgtggctca
     4081 gatcgagcac gatcccgagc gtgtccgagg tcgccgccgc ctcgagagcg gcacccagct
     4141 cggccgcgtt cgagatgtcg atctcacctt cgaggtgcac gaccagcgcg tcttgcacgc
     4201 gctcaacgtc gagctgtgcg agcggcttca tgcggtcacc ttcgtcttga gcccacggcg
     4261 cagccgcacg gtcgtgccct gcggtcccgg cgtgatcttc gccgagtccg tgagccgcct
     4321 catcagctcg agcccgcggc cgcggtcgct cttgcggggc ggccgccagt agccgaagtc
     4381 gcggatcacg agctccacct cgccgttctc gtgcgtggcc tcgagcacga acacggcgtc
     4441 gccgcccggg taggcgtgct ccaccacgtt catgcaggcc tcgccgcagg caacggtgag
     4501 ctcgtacatc acctggcgct cggcaccgtg agcgtcgagc cagcgctcga gcgtgcggcg
     4561 gaccgccacc atcgactcgg gctccgccgg caagcgcagc tcgaacttgt tgctcggcac
     4621 gggatcgagc cgcgccgcga gcaccgccac gtcgtcaccg gcgatgccat cactgggaac
     4681 ggtctcgagc acgtggtcgc agagtgcgtc cggatcctcc ggccctcctg cgacggcgtg
     4741 gctcagccgc tccatgccat cttcgaccga catacctcgc tcctctacga gcccgtccgt
     4801 gtagaggatg agcgtcgagc cgggctctat agccacgacg ctctccctgt aggccccgaa
     4861 cggcaggacg ccgagcggca cgtactcccc ggttgggagg aatgaagttg tcccgtcggg
     4921 cccgcgcacg agcggcggca gatgaccggc ggtcgagtag gtgagagtgg atgtgcgcgg
     4981 gtcgaccagg acgtagagga gcgtggcgat ctgcccctcg tcccagtgcc cgaccacgcg
     5041 gttcaggcgc tccaccacgt cggccggctc gccgccctcg atcgcgtagg cgcgcagcgc
     5101 gttgcgaagc tgccccatga gcgacgcggc ctcgatgccg cgcccgacca cgtcgccgat
     5161 cgcgaggccg atgcggccgt cgaggtactc gacaacgtcg aacaggtcgc cgcccaccgc
     5221 ggaccccgcc gcgcctggct ggtagcgggt cgcgagcgtg agccccggga tgtccgggat
     5281 gcggctcggc aggaggctgc gctgaagcgt ctcggcgatg cggtgcgcct gctcgttcaa
     5341 ctgcgcgcgg tcgatcgcga ccgccacgcg gtctgccgcg agctggagca ggcgcgagtc
     5401 gtcctcgctg aagcctgcgt cgtcgctgct cgccacctgc agcacgccga tcacggtgcc
     5461 gccgcgcacg agcagcggca caccgagcag cgagctcacg tccgcccccg gcacgagcgg
     5521 gctcgcttcg ctcgcgtcct ccatcgcgcg gatgaaaacc gggcgcttct cggcggctac
     5581 acgaccggcg aggccctcgc ccagcggcac gcgcctgcgc ttcagctgct cggcatcgcg
     5641 gccgtgggcc gcgcggacgc gcagcgcgcc ggtgtcgccg tcgttcaaga ggatcgcggc
     5701 gcgctcgacc tggagcacct tggtgatgcg accgagcagc tcggagagca ggtcgtcgaa
     5761 cggcagatgc gcgagggcgg cgtcggacac cgcctcgagg ccctgcacgc ggcgcaccgc
     5821 ctgctcggcg cgctcgagcg cgtcacgctc gagctcgtgc atctcacggc gctgcgagcg
     5881 cacccgcgag atgagcgcaa gcagtacgaa gccgaccgcc acccacagca ccgcgttggt
     5941 gacagtgtcg atcagcgtgc gagtctcgtg accggcatag agcagcggca tgcaaccggc
     6001 cgccaccacc gcaccaatga atggaacgcc cctgcgcggc ggatgcacgc cgacgcccgc
     6061 gccgagccaa agaacgaaca gcgcgcggta ggcggagtcg ccaccggcga gccactcgag
     6121 cgcggcgatc tgcgtcacac cggcgtaggt ggcgatcagg agcccgtcga agccgggctc
     6181 ctcagcgtcg ccaagcaccc agcgagctcc gaggaaggag gcacccacta cagccacacc
     6241 ggcgagccag cccgcggccc cgatggggct ctccgtgggc ggctcgacca gcagcaaccc
     6301 gagcgcgatc gtgcctgaca ccgcccacag cacgcccacg aacttgcgcg cggtcaagag
     6361 gtcagcgccg tcgtaggcct gctcgctacg tcgcagcagg tcccggatcg ctccgaaact
     6421 gggcaggggg aacttgcgct ctgccgacat gcggtcctat cggctcgcgc gcgtgcgcgc
     6481 atgacagccg ggagggctgt cccatccatt cgtctagcag gcgaagctgg cgctaaggaa
     6541 ctcgcccgag ccgctcgagc agctcggccg tcgcgacgcc gtgggcgcgc agcacgtcgt
     6601 cgaagagcgg tccgtacagc tcgatcagtg ccgagagcag atcggcggtc gagacgatct
     6661 catcaccgtg ctcgccggcg attcgcgacg cctcggagta caccgagtgg acgcgaacgc
     6721 cctcggcctc gtcggcgccg gcgtcgagct ccgacagcgg acgctcgccc gcgccgagcg
     6781 cctgcaacgc gctgccgacc tcgccgtgca tcctcaggat gcggatccag tgttcgacct
     6841 ccgccgacgg cgagtcggcg aacggtagag ctgtctcggc gagcgcgatg gcaatcgccg
     6901 cgtcttctga tggtggtagt tcacgagtcc ttgcgggcat gtcccctctc ttcccctaca
     6961 cccgtcaccg aaagcacacg ctggacgcat gtacgaccgg gacggctgcg tccaaaatca
     7021 tcctcgcagc ccgcgatcgg gcggatcctg ttcgagcggg ctgatgtggc gcgccaccgc
     7081 ctgccagcga ccgtcgcgtc gcaccagcac gtcactcatc agatacgcgg tcgtccggtc
     7141 ctcccctccc atgcgaccgc gctggcggta gtgggagcga accagcgcag cgtcgccgta
     7201 cacgcgtgcg tccacgtcgg cgaactcgaa cgagtcgatc gcataggagt cgcgggtcac
     7261 gtcgagccac tccgctcgcg agcgtacctc gacgcccggc cgcccggtgg tgagcgtgaa
     7321 ctcttcgccg agcagccgct cgaggaactc catgtcgcgc gtgcgagtcg cttcgagcca
     7381 tgcgtactcg agatcgacga gttcctggat cgcatcgtct gcgcccaccc ggggaagtat
     7441 tccggtgacc cgggacggca gtcactgcgc cgacccgcgc tcgacgtgag tcccggcaga
     7501 ggcgatgatg cgctatccgg cgatggcgat gcgagtgaga ccgaaggcag cggagcggtg
     7561 agcgcgaacg tcgcgaggcg ggcggtgctc gggctgctcg gtcgcctgaa gcgcgggcgc
     7621 gtagagctcg tcgacgccgg gcgccggctc gagttcggcg acggcgacgg cccggcagcg
     7681 acagtgaccg tgcgctcgcc cgccttctac cgccaggtgc tgcgcggcag cctcggtatc
     7741 ggcgactcct atacgcgcgg gctgtgggac tgcgacgacc tcgtggcgct ggtgcgcgtg
     7801 ctcgcgcgga acatggaaac gatcgaccgc gtgcgccggc gcatggcgcc ggtgctctcg
     7861 ccggtgcagc gagttgcgtc ccgctcgtgg cttccaacgc ccgcgcgcag caggcgccag
     7921 atcgccgcgc actacgacct cggcaacgac ttcttcgagc tcttcctcga cgagaccatg
     7981 acctactcgt gcgcggtatt cgacacgccc ggcgccgatc tggccgatgc gtcgcgcgcg
     8041 aagctcgagc gcgtctgcgc gaagctcgac gtgcacccgg gcgaccacgt gctcgagata
     8101 ggcaccggct ggggcagctt cgcgctccac gcggcaacca ctcgcggctg ccgcgtgacg
     8161 accaccacgc tctcgaagga gcagcacgac tacgcgcgcg agcggatcga agacgccggc
     8221 ctgcgtgacc gaatcacgtt gctgctcgac gactaccgcc atctgcgcgg gcgcttcgac
     8281 aagctcgtct cgatcgagat gatcgaggcc gttggctggc agaacttcgg gacgttcttc
     8341 cgccgctgct cggagctgct caagtcgacc ggcgcgatgg cgctccaggc gatcacgatc
     8401 gatgatcgcg cgtacgagct cgagaaggcc aagcggacgt tcatcaagtc gcgcatcttc
     8461 ccgggcggag cgctgccgtc ggtggaggtg atgacgcgtg aggtggcacg cagcaccgac
     8521 atgcgcgccg tgcacctcga ggacatcacg ccgcactacc cgcccacgct cgccgcgtgg
     8581 cggcggcgcc tgctcgagaa cgcaggtcgc gtgctggcgc gcggctatcc cgagcggctg
     8641 gtgcgccagt tcgagctcta cttcgcctac tgcgaggcag ggttcacgga gcggcggatc
     8701 ggagacgtgc agatggtgct ggcgaagccg gccttccgcg gcgagcaggc cgtgctcgag
     8761 ggcgcgcgcc atgcggtgct cgccacatga acggtctcaa ggtcgccacc gccgtcttcg
     8821 ggtccgccag ggcggcgatc ggcgccggcc tcgcggttga cccggagcgg ctcggcaggc
     8881 gctggatcgg taaggacgcg cgcaggccac gcacgcaggt cgcactcagg gggctcggcg
     8941 cgcgtgacgt ggcgatcggc ggcggcaccg tcgcggccgc gctcaccgag gatgatgtgc
     9001 tgctctggct ttgcggcggg atcgtctccg acgtggccga catcgcggcc acgctgtcgg
     9061 agcgcgagaa cgtgccgcgg cgcggcgtca ggctcaccgc cgtgctggcg ggcgtgggcg
     9121 tgatcgcggg ggcggcgctc gcgggcgcct gggcgcgcgg ctaggcggcg cgcgcgagcg
     9181 cggccggctg cgtgcgctcg agcacgcgca tcggcagtcc gtcgatcgga ctcagcgtgg
     9241 gcatctgacg gatgcgcatg cggtagccgg gctgcagctc gaggcggcag cgctgcagga
     9301 tcagcgtcgc gatcgtcttg atctcgagct gaccgaagcg catcccgatg cacgtgcgcg
     9361 agccgccacc gaacggcacg tacgcgccct tgggcagctc ggccttcgcc tcgggagcga
     9421 agcgctcggg cacgaaggcc tcggggtcgg gatagacctc cggcaggtgg tgggtcaccc
     9481 acgaggagta gttcacatag gtctccgcag gcaccctcac cccgcacagc tcgaacggct
     9541 cgacggacat gcgcggcccc acccacgccg gcgggtagag gcgcagcgtc tcgtcgaccg
     9601 ccatctcgag ctcaggcagc gcctcgccgc ccatcagctc ggcggcggtg gccatgcggc
     9661 cgccgagcac gcggtcctgc tcctcgatca gtcgcgccag cgcctgcggg tgcctcgcga
     9721 gctcgtagaa caggaaggtg acggtcgagg tggtggtgtc gtgccccgcg aacaggagcg
     9781 tcatcacctg gtcgcgcaca gccctgtcgg agagcgtgtt gccgtcctcg tcgctcgcct
     9841 cgagcagcag cgacaggatg tcgtcgccgg cgtcgccgct cgtacgacgc cgcgagatct
     9901 gaccgaatat cagccggtcg agtcgctccc gcgccttgat catcctcgcc cagggtgtgc
     9961 gcgggccgcg caggatctgg atcgcgtagt ccgtcgcgta gtaggagagc gcctcctcga
    10021 actccgccgc ctcgtcgaag ccgtgcgact tgtcgtcggg gttgaacccg aacagagccc
    10081 gcatggcgat cctcatcgcg agcgcacgcg tccagtggta catgtcgacc tgccgcccag
    10141 gctgccagcc ctcgagcgcg cgctcggcct cctccaccat cgtgccggcc gttgcagcta
    10201 tgcggtcgcg gtggaagacg ggcagcatga gcttgcgcga gcggcggtgg tagtcgccgt
    10261 cgatcgtcag caggccgtcg ccgagcagcg ggatgaggtc gccgaagctg ccgtcgcgcc
    10321 agcggaagtt ctgcgcgtgc gagacggtca cgtagtggtt cgcctccggg ccgatcacga
    10381 acaccaccgg ctcgtgcagc aggcgcatcg agaagaccgg gccgtagcgc tcgtagcact
    10441 cgagcaccag tcgcagcgga tcggtgcgca tgcgccgcgt gcgcgcgatg ctgaactgca
    10501 gctctcccgg gggcagcggc tcggtgcggc ggcgttccct ttcgaggtcc atccgcagtc
    10561 gcttgagcgg gttcatcgag atctgcggcg ggggagccag cgccatgggc gaaagcgtac
    10621 ccggccctgg ctggctggcc aactcggcgc gcttgtcggt ggcggttgcg aacgctccgg
    10681 tgcatagtgg cctccgatgg gctcgcgccg tctcttcgtc ttggcgtgcc tcctggcact
    10741 gctggtggtt ccgcccgcgg cacattcggc gccgccgccg tccgtcgccg actactacct
    10801 ggatgccgac gtgggcagcg acttggagga ctgcctcggc ccaaagggcg cccccgatgg
    10861 ccacgcgccg tgcgcgacgt tcgatcggat cgagcagctg aatccgtcga gcgtcgtgac
    10921 gatccacctg gcgcccggcg agtacgacgc gccgtcgagt gcaccgagcc agttcatgta
    10981 catcgtcggc gacacggacg cgacgatcac gtccggcctc gagttcgagc acggcgcctc
    11041 gctgtcgaat gtgacagtca cgagcgcaga cgatcccggc gcccccgcgc tcgaactgcg
    11101 caacgcggac acgagcccgg cggactacct actcgacggc gtgaccgcag tcgccagcag
    11161 cacgggcggt ccgagcgcga atgcggtcga ggtcaccggg cccgtcgacc tcgccgccac
    11221 ggacagcgtg ttcgccgtgg gcgattcggg ctgggtcgtg cacagcgacg gcgccgacgt
    11281 gtcgctgcag ctcaacggga cctcgctgca ggtcggcgcg ggcggccgcg ggctgcagat
    11341 cgacggcggc ggcgaggcgg ccctcacgga cagcacggtc gacgggcccg gcgcggcgta
    11401 cggcatcgtc gtgaatggcc tcagcgccgc gctcacactc gagcgcagct cgatcgcggc
    11461 gggcagcacc gcggtatccg ttcgacatca ctttgggagc ggcaccacca cagcgaacgt
    11521 gcgcgacagc gtgctccagt cgatgtcggc cagcacggcg cctgacgacg gcgcgctcgt
    11581 cgcgtccacg cagctgagcc cggccgacca gagcccacac gtggcggtga cggtcacggg
    11641 ctcgacgctc gtgaacatgg ccgacgcgga cccggaccac gggcccgacg gcgccgcgat
    11701 tgcttaccag agcggcggca ccgcgacgat cgcgctccac aacaccctcg tgtacgcgcc
    11761 gcacctgcca gacctcacgg cgatccagtc gggcatctat ccggccggct cagccgccat
    11821 caccgcggac cactcgatct tcgggactcc gacgtcgttc gagggcgcct tcgcggcgga
    11881 caacggcgcg gatggcaaca gcggcgcgga tccgctcctg gccgatccgg aggacggcga
    11941 cttcacgctg ctcgccggct cgccggccat cgacgcgggc gacgcgtcgg cggtcaccgg
    12001 gggcgagagc gacgtgaacg gcgcgccgcg ctcacaggac ggcgacggcg actgcgtgtc
    12061 gttgccggac gtgggcgcat tcgaacgcag cgccaccctc acctgcacac ccaagtccac
    12121 gccgccgggt gaggccgatc acgcgacggg caagccgacg gacaccacca agccgaaggc
    12181 gcacgcatca ctcaggcggg cgctgaagct ccgcactgcg ctgaggcgcg gcgtccgcac
    12241 gcgcgtctac tcgaacgagc cggccgacgt gaacctcacc gccgagctcg gaccgcggag
    12301 cgcacacaag ctcggcctcg cggcgaagcc ggtcgtggtg gcgcgcgcga agacggcgct
    12361 caacggcaat gccgcccgtg tggtcacact caagttcacg cggaaggcga agtcgaagct
    12421 gcgcggcgga aagacgatca agctcgcgat acgtatcagc gtgcgcgacg tcgccggtaa
    12481 cacgagcacg ctcgtccgcc acatcacgct caagcggtag ctcagcgggg cagcagcggc
    12541 gccacgatcc ggtacgcctt gaggccgagc ccgaggcgct cgcgatcctc ggaccggaac
    12601 gggtcgagcc cggtgagctc gcgcacgcgc tcgagtcggt agcccaccgt gtgccggtgg
    12661 gcgtgcacgg ccgcggacgt tgccttgatc gagcagttgc tgtcgaggta cgcgctgagc
    12721 gtgccggtga gatcgctgga gtacgtctcg tcgtagcgaa ccaacggcgc aaccgtgtcc
    12781 tcgtagaagc tccgcacctc ctccgggtgc gacgcaagta cgcggaacag aaggcgatac
    12841 gcgccgccgt ccatctctcc atcgcgcggc tcgacgcccg cggagcgcac ctcgagcacg
    12901 agctcggcct cttcgagcgc ccgacgcaga gcacccgggc tcgagtagcg cgaggacacg
    12961 ccgacggtgg cctgcctgcc caggcgggcg gccagagcct gcgcctcctc gatacttcca
    13021 ggcacgagtg cgaacacgcg cccgcgcacc acgtccgcca ccgcgtctgc ccgctccgcg
    13081 gagatcactg cgagcaagcg ccccggcgtg cgctcgcccg gatcggcgca gatgccgacg
    13141 ccgccggacg acaggtcgat ccccatgcgg cgtccgcgcc gcacgacgtc ttcggccgcc
    13201 acgtcgtcgt tctcgagcag ctgctcgatc agcgacccgc gcagcgactg ctccgcctcg
    13261 gcgtgcgcct ccgagacggc cagcgaggtg agcgccgcga gcgccgccac ctgcgcgtac
    13321 tcgcctgcgt cggggcgtcc ctgccccagc aacaggacgg acccgacctc acgccccccg
    13381 gtggcgatcg gcacctcgtt caccacgccg gccggccgtt ccacagcctc gccggcgagg
    13441 cggcctgcca cgtagcgctc gtactccgcc catgcgggcg gagcacctag ctgcggcacg
    13501 atcaccgcca ccggtgcccc tgcatggcgt tcggcgatct cggctatccg ctcgagaccc
    13561 tgccccgcca gcacggcgcc caccatgtcg aggtgcgcgc cgcgcggcga gggtagggtg
    13621 cgcgggagag aggcggtcag gcgggaatct cctcggactc gtgagcctgg aggacttcct
    13681 cgccggactc gcctgttcgg atgcgaatcg catcctcgac ggggagcagg aagatcttcc
    13741 cgtccccgac gtcacctgtg cgcgcgtggc gcaggatcgt cccgatcacc tgctccacgt
    13801 ccgtcgtctc gacgacgcac tcgattttga gcttcggccg aaggtgaatc gcgagcgtcg
    13861 caccgcggta ctgctcggtg atccccttct gcctccccga gcccttcacc tccgtgatcg
    13921 agaggctcgg gaaaccggcg tcgagcagct cttggcgaat cggctcgaac gcctcgtggc
    13981 ggatgtaggc gacgaccatt ttcatgctgt cgctccatcc ctcgtcgtca tgagcgccgg
    14041 actcgggacc ggtgcgggct gcggcggcac cgcaagcgga ccgctcgacg ccgcgccgat
    14101 ctccggcgcc gggatgaact gctccgggta cccgtacatg ccgtgctcgg cgatgtcgag
    14161 gcccgcctct tcctgctcgg gcgtgacgcg caggccgatc gtcttcttga tcgtccagaa
    14221 caccacgtac gagaccgaga acacgcaggc aaacgcgacc gccacaccca gcgcctgggc
    14281 gccgagctgg tggaagctgc ccgagtacac gaggcccgga tcgccgaccg cgttcagcgt
    14341 cgcgagccgc ggcgacgtga acaggccgca cgacagcgtg ccccagatac cggccaggcc
    14401 gtgcgccgac agcgcgccga ccggatcgtc caggaccttg tcgatggcga gcacacccac
    14461 gaccacgatc acgcctgcga ccgcgccgat gatcggcgcc gcccagtact ccacatagcc
    14521 ggacggcgcg gtgatcgcga cgagcgctgc gatggcgccg ttgccgacca tgccgatgtc
    14581 gaccgtcttg cggagcaggt ggatcgttgc cgccgcggcg atcacgccgg cggccgcggc
    14641 gaggttcgtg acgacgacca cctcagcgaa gcggttgccg atcgcgccta gcgtggagcc
    14701 ggggttgaat ccgaaccagc ccagccacag gatcaggatg cccaggccga cgagcggcat
    14761 ggagtggccc ggaatagcgc gcggcttgcc gttctcgtcg tacttgccgc gtctcgggcc
    14821 gagtaacagc agcgccgcga gcgccccggt cgctccgatc aggtggacga ctgtcgaacc
    14881 cgcgaagtcc tgcatgccca cgctcacctg gagccagccg ccaccgaaga tccagtggct
    14941 gaggatcgga tagatgagcg cgctgaagat caccgcgtag atgacgtacg cgctgaactt
    15001 gatgcgctcg agcgtggtgc cccacacgat cgcgagcgac accgcgcaga acacgaactg
    15061 gaagaacagc agcgcccccg ggctgatgtc ataggtgccg aggagcggga tgtccttcgc
    15121 ggcggccgcc gcggtgtggc ccacgttcag gaaccagccg tgggtgccgg caatggcccc
    15181 cgagccaccg aaggcgaggg cgaagcccac cgcccagtag gcgatggagg cgatcgagaa
    15241 gttcatgagg atcttcgcga cgcccgcgcc cgcgttcttc atccgcgaga agccgatctc
    15301 gaggagagcg aagccggcct gcatgaacat caccaggatg ccggcaacta tcacccaggt
    15361 cgtgttcacg ctcgacacgg tttcggtcag cgacggaccg tccgccgcaa aggcgcccgc
    15421 cgggacggcg agcacggccg cgatgctcag ggcgaaggcg cccagggcac gacggcggag
    15481 agtgggtctc attctgcgag ccctccttct gcacccccac ggcacccgcg caggcccgcg
    15541 tcacgaggcg cgttcgacga cgcctcgcaa cctaagtggg ctctgtccag cggtctttgt
    15601 cccgctgtct gaaattcagg cggctatggc cgcccgtgtg tcgaaaggcc ctgcaagcgg
    15661 cctagctgcg tgagcgctcg cgggcccggc gcgtctcgcg ctcgttgtac ttctcgagcg
    15721 cgtctacgag ctcctccttc gacatgtcgg agcgcccctc gatgccggcc tctttcgcct
    15781 cctggtacag ctcgtccttc gacttggtcg cgtccacgcc gcccgcggtg ggcttcgggt
    15841 tgtcgcgcgc cgcctggccg ccgcgcgcgg cctgctcatc cgacggtccg ggctcgtcct
    15901 tcagctccca gtggtcgccc ttcttctcgg cgatgtgctt caccgcggcc caggccacgc
    15961 ggtgcgcgcg ctcctcgtcg ccctcgtact gctcgtgcgc gctgttgagc gtctcttcgt
    16021 aggtgtcgcg caccttcttg ggcgagcgct cgatcgtgct gggaatgtcc tcttgcctcg
    16081 ctggcacgtc ggctcctttc gtcgtctcgg tgaagggcta ccgcggcctg ccgtgccgca
    16141 aaccgggcca acgacgagcg ataaggagaa cagacgtgag taccacggct gggctaccgc
    16201 acgctaggcg tgggtgactt tagggggtct atagggcacc taaagtcacc cacgtccacg
    16261 cgataccgcg attggagtag cccagttcgg cctgtgccgc cctcattgct gctgtaaacg
    16321 cgacgagctg cgggcacgcg gcgagtgtgc ccacgggcgc gcgcgggcgt tgatagcaat
    16381 ccgatatcgc gatatcctcg cccgcatgtc cgacgacgtc ctcatctact ccgaccgtcc
    16441 gctctacgtc cggctgccag agcgcgaagc cgagatgctc gaccgcgccg cccgcgaggg
    16501 ccgcacgtca aagcggcagc tcgtcacctc gctcgtgcag cgcttcctcg gcgaggaggg
    16561 cccgctcgtt gtcgacggcc gcggagtcgc cgagacggga cgccacgact tcagtgccgg
    16621 cagcgcgccc gaggtactca cgctggagca agccgccgag ctgctgatgg tggcgccgga
    16681 agcgatcgag gagctcgccg cgaagggcga gctgcccggc cgccggatcg gcggcgagtg
    16741 gcgcttctcc cgctccgcgc tagtggactg gctcgccgcg cgctaagcga gcaggtggcc
    16801 gaccctgcgc aggcgctcgt cggcctccgc gggccaggcg gccgggtcgg ccggccacca
    16861 cgcgaactcg tcgtgctcgt ggtcgggcac cgcgtcagcg ccggcggcca cgtgcgccag
    16921 gccgacgagc atggccaggc ccgtcgggga ctgcacgagc gcctcgaccg acatgcgctc
    16981 gggagtgagc tgccactcct ctacgagctc gcgcgacagg gtgtctgccg ggttctcttc
    17041 cacctccacc gcgccgcccg cgccgagcgc ccagcggccc gcccaggacg caagccagtc
    17101 cgcacgcctg cccgcgagcc agcggccgtc gccgtcccgg acgacggtga gcgcggtcat
    17161 gctgcccgcg gccgcctcgt ggtccacgag gcgaagcgcc cagcgcgccg gctgcagctc
    17221 gagggcgagt gtgcggtcgt tgccgccgaa accggctagc cgtgcggcca ggccgtcgtg
    17281 ggcgggcgac ccgcgctcgc tgagcgcacg caccgcctcg tcggccagcg cgtcgacctc
    17341 gggcggcggc tcgtactggt cctcgcgcca atgcaccatt acgctctccg gcggccacgg
    17401 cccgcgcgca agcatgctcg ccggcgctct gctcacgcgg ctcccgccac cagccgctcg
    17461 ctcaagtccc acagctgcgt gcgcgcagcc ggatcggcgg cctgcgggtc gggcctcgtc
    17521 tcgcgcatgc cgttgaagta gcgcccgctc acgccgtcga gcgatggatc ggcgatcagc
    17581 cgctcggtcg cgtcagcgcc ctggcgcacg gtgctcgtaa tcggcgcgcc cgactcgcgg
    17641 accatgttcg tgtccatgta ggtggcggga tgcagcgcag tcacggccag ctcgtcgggc
    17701 ggcacgcgct cggccagctc cgccgtgaac atgatctgcg cgagcttgct ctggcagtac
    17761 gcgcgcacgc cgctgtagcc gtgctcgagc atcgggtcgg cgaagtcgat cggcatctgg
    17821 cccgccgagc tcacgttcac cacgcgcgcg ggagcgctcg cccgcagtag cgccagcagc
    17881 tcgatggtga gcaggaagcc ggcgaggtag ttcaccgcga agcgcagctc gaggccgtca
    17941 gcgctcagct cccggcgcgt gccgtcgctt cccgagccga tccccgcgtt gttcacgagc
    18001 acatcgaggc ggtcgtggtc cgcgcgcaca tcttccgcga ggcgccgcac ctccgctagc
    18061 gaggagaggt ccgcgacgta ggactcggcc ttgccggagc cgcggcgtat ctcgtcgacc
    18121 gtgtcctcga tcttctccgg attgcgcccg tgcacgagca cggtcgcccc gcgcccggcg
    18181 aggtcgagcg cgacttggcg ccccagcccg tccgtcgcgc cggtaacgag aatcgtctgc
    18241 tcctcggtcg gccgcacggc tccaagtatg tagaggtcct cgaccggctt gctcccgccg
    18301 cgctcgcttg cgcaccaggc gcgcgccccg tacactcgcg cgtaactggt tggaccagtt
    18361 atgaacgagc tccggaccga agaccgccgc ctcgcctcgg acgccgtgca cgagcggctg
    18421 cgcgccgaga tcctggacgg tcgccttgcg cccggcgacc cactgccgtc ggagcgaacg
    18481 ctgagcgccg agcacggcgt gaaccggcac gccgtccgcg aggcggtcaa gcggctgcag
    18541 caggcgggcc tcgtgcgcgt gtcgcacggg ggcgccacgc gggtgctcga ctggcgcgcg
    18601 acagggggcc tcgacctgct cgccgacctg agcatgagcg ggcaaggaca cgacttgctg
    18661 cgctcgatcg ccgagatgcg cgcctcgatt ggcgcggacg ccgcacggct atgcgcgcgg
    18721 cgcgcaccgg ccgacacgcg cgagagactc acccacctgg ctgccccgcg taaggacgcg
    18781 agctacgagg agcagctcgt ggcctacgag gagctgtggc agttgatcgt cgacgggagc
    18841 ggcaacctcg gataccggct ggcgttcaac agcctcgtgg gcgcgcggca cgggcacggc
    18901 gtgaactcgc gcatctacgc ggacgaggtc ggcgatgtgc gcgcgtggcg cgcgctcgcc
    18961 gcggcggtga cggccggcga cgagcc
//
LOCUS       DASVXS010000160         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1387872, whole genome shotgun sequence.
ACCESSION   DASVXS010000160 DASVXS010000000
VERSION     DASVXS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387872"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /gene="gcvPB"
                     /locus_tag="VF752_08920"
     CDS             <1..1058
                     /gene="gcvPB"
                     /locus_tag="VF752_08920"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX8855712.1"
                     /translation="GLLLTRAYHEERGQERHKVLTPDTAHGTNPATVTMAGYEVVKVG
                     TAPDGGVDLDDLRAKADEDVACLMLTNPNTLGLFDTNIREIASIVHDVGGTLYYDGAN
                     LNAVMGISRPGDMGFDIVHFNLHKTFTQPHGGGGPGSGPIAVSDRIEPFLPRPQVVRR
                     EASNGSDRDYFDLDYERPKSIGRLRGFQGNFGVFVRSYAYIRSVGGDGLRDVSEIAVL
                     NANYLRARLAEEGIAEYLPIAFDRVCMHEFVLSGRGAKEKLGIKTLDIAKRLLDFKMH
                     PPTVYFPLLVDEALMIEPTETETKETLDRFAEVIREIVREAADDPEIARNAPYSTPVR
                     RLDEARAARQPIVRQPR"
     gene            complement(1281..1484)
                     /locus_tag="VF752_08925"
     CDS             complement(1281..1484)
                     /locus_tag="VF752_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855713.1"
                     /translation="MPPWRQAVYWLASIAALGLVIWDIVDKSAQYTSIVVIALIVIAT
                     VALWAPDPQRGEGHERAEQPPRG"
     gene            complement(1883..2203)
                     /locus_tag="VF752_08930"
     CDS             complement(1883..2203)
                     /locus_tag="VF752_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855714.1"
                     /translation="MAATDVTRAREGARPGSHGIVRRLATETKAAVKTTEFYAYIAVL
                     AAILIAGLATKAGSGHDDRLLSNQVWLFATILTVGYMVSRGLAKSGSREPYTEDLDGR
                     KSDR"
     gene            complement(2403..3032)
                     /locus_tag="VF752_08935"
     CDS             complement(2403..3032)
                     /locus_tag="VF752_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8855715.1"
                     /translation="MDSRRSSSLGRPVPVQMPDRAERLRAVFEQSRNAMLIADDERRY
                     RDVNHAACRLLDAPRGWILKRRIDDLTPPELRTGVEAMWKAFIAEGSQAGDFELLLPD
                     GRRRTVEYSATANILPGMHLSILLPLVAGRAGGDATQAREPVSLTPREAEVMGLVALG
                     ASTQELAERLNVSQETARSHTKNALEKLGARNRAHAVALALSRGELTLD"
     gene            3305..4084
                     /locus_tag="VF752_08940"
     CDS             3305..4084
                     /locus_tag="VF752_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX8855716.1"
                     /translation="MLDLYDRPAPTRPAPRSATGAADVLFARYRRGDFAARRLLIERF
                     LPLARNLARRYEGRGEPLDDLVQVASVGLVKAIDRFEPGRGFKFSSYAVPTMLGELRR
                     HFRDSGWAVHLARDMQERVLKVQAAVERLSGQLGRSPNPQQVADELKMSVEHVLEAIE
                     ANSSYKAASLDAPAPSSLDDGGNQADMVGVVDERFELIEAAASIAPALRALPERERTI
                     LYLRFVKDLSQTQIAKQIGASQVHVSRLIRQALEDVRSETE"
     gene            4348..5334
                     /gene="trpS"
                     /locus_tag="VF752_08945"
     CDS             4348..5334
                     /gene="trpS"
                     /locus_tag="VF752_08945"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX8855717.1"
                     /translation="MRIFSGIQPTGRKHLGNYIGAIRQYVDGQDRGEAIYCIVDLHAI
                     TVAYDPKELRERVYDTTAILIAAGLDPARCILFRQADVPEHTELCWLLSGVTAHGDLN
                     RMTQFKDKSARQRELVSAGLFLYPVLQAADVLAYNADEVPVGEDQRQHIELMRDIAER
                     FNERFGDTFVVPRAVIPEVGARIMDLQQPTAKMSTTAPSEAGTILVLDEPKTIERKVK
                     SAVTDSGSDVLRRPGKEGISNLIEILSVIRGVSPDAVEAEFDGSGYGGFKQAVADAVV
                     EYLRPVRERYEEIRADEGELERILAEGAGKAHAIAHDIVAEASDKMGVGPPR"
     gene            5345..6067
                     /locus_tag="VF752_08950"
     CDS             5345..6067
                     /locus_tag="VF752_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX8855718.1"
                     /translation="MPVASLDLDLEVFQGPFDLLLTLVLKEEVDLREVDLAEVVLAYI
                     DHLEAQGELDLEATTEFLVLIAALLELKSRLILPREEEEEIDLPQDEAADELLARMLE
                     YTRYRRAASWLHEQLSDESGFRYRSAPLPPELRRVSVDLAEKSYEPVKLAEALGGLLR
                     KPEPLTLRHMATPIVSVEQRLSVLRGLLARSRSFSFDEAVRGADRLTEAMTLWALLEL
                     YKQGEAQWQQDENFGPIRVSAP"
BASE COUNT          988 a         2105 c         2073 g          944 t
ORIGIN      
        1 ccggcctgct gctcacgcgc gcctaccacg aggagcgcgg gcaggagcgc cacaaggtgc
       61 tcacgcccga caccgcgcac ggcaccaatc ccgcaaccgt gacgatggcc ggctacgagg
      121 tggtgaaggt gggcacggcg cccgacggcg gcgtcgatct cgacgacctg cgcgcgaagg
      181 ccgacgagga tgtggcctgc ctgatgctca ccaacccgaa cacgctcggt ctgttcgaca
      241 cgaacatccg cgagatcgcc tcgatcgtgc acgacgtggg cggcacgctc tactacgacg
      301 gcgcgaacct gaacgcggtg atgggcatct cgcggccggg cgacatgggc ttcgacatcg
      361 tgcacttcaa cctgcacaag acgttcaccc agccgcacgg aggcggcggg ccggggtcag
      421 gcccgatcgc ggtgtccgat cgaatcgagc cgttcctgcc gcgcccgcaa gtggtgcggc
      481 gcgaggcatc gaacggatcg gatcgcgact acttcgacct cgactacgag cggcccaagt
      541 cgatcgggcg cctgcggggc ttccagggca atttcggcgt gttcgtgcgc tcgtacgcgt
      601 acatccgctc cgtgggcggc gacggcctgc gcgacgtgtc cgagatcgcc gtgcttaacg
      661 cgaactacct gcgcgcgcgg ctcgcggagg aggggatcgc ggagtacctg ccgatcgcgt
      721 tcgaccgcgt gtgcatgcac gagttcgtgc tgtccggccg gggggcgaag gagaagctgg
      781 gcatcaagac gctcgacatc gccaagcggc tgctcgactt caagatgcac ccgccgacgg
      841 tgtacttccc gctgctggtc gacgaggcgc tgatgatcga gcccactgag accgagacca
      901 aggagacgct ggatcgcttc gccgaggtga tccgcgagat cgtgcgcgag gctgctgacg
      961 atcccgagat cgcgcgcaac gcgccgtact cgacgcctgt gcgacggctc gacgaggcgc
     1021 gggcggcgcg gcagccgatc gtccgtcaac cgcgctagtt cgggcgtttc cggcacccgc
     1081 ccgccgggtg ctcgggccgc gcctctgtcc aagcccgtta ccgctaactc ctgactttgg
     1141 ttctcatatg cggaaccaaa ctcaggagct ggccggcgaa tcgccgtgcg tcgtcactca
     1201 ggggtgtata tgcaccccga gcgacgaccc ccatccgtta ttgctgctgt tcaccgacgc
     1261 cggaagccct tcgccggcga tcacccgcgc ggcggctgct cggcccgctc gtgcccttcc
     1321 ccgcgttgcg ggtcaggtgc ccacagcgcg accgtggcaa tcactatcag ggcaatcacg
     1381 acgatgctcg tgtactgcgc gctcttgtcg acgatgtccc agatcacaag gccgagcgct
     1441 gcgatcgagg cgagccagta gaccgcctga cgccacggcg gcaaggtgcg cagaaccctc
     1501 agccagcgca cgcgccggtc gcttccgtgg agcggttgac gactgcgtcg tccccgggat
     1561 gagcgaggat cgaagggcct cggcagccca tatcgcgacc atacctcatg ttgctggcgc
     1621 cgaagcactc aggctcgtgg gcttccgctt ctcatcaaaa tcgcgcgcgc gagcggtgct
     1681 cagcggcgat gtcggtcttg cctcgacgca gcagcggatc tgcacccgcc agcctagaac
     1741 cagccgccga accaaccggc aaggccctgc tgctcgacgc ggcggggcgc ccgcgctcgc
     1801 cagcggacct cgactatcgc cgcgccctca accggccccc ggacaggacg ctcctgtcca
     1861 ggggccggcg ggggaagggg gcctagcggt cgctcttgcg gccgtcaagg tcttccgtgt
     1921 aaggctcacg gctgccggac ttggcgaggc cgcggctgac catgtagccg acggtgagga
     1981 tcgtggcgaa gagccagacc tggtttgaga ggaggcggtc gtcatggccg cttccggcct
     2041 tcgttgccaa gcccgcgatc aggatggctg cgagcacggc gatgtaggcg tagaactcgg
     2101 tcgtcttgac cgcggccttc gtctcagtcg ctagtcgacg gacgatgccg tgcgatccgg
     2161 gccgagcgcc ctcgcgggcg cgggttacgt cggtggctgc cattgagcag tctccttgct
     2221 tgggggatcc gtggggtact agccactgcg tacgccagac gggcggtgcc gctgcactcc
     2281 acgagaacag acacggccgc tctggctact gcgtgcttcc caaagggtgc ccaccaaggt
     2341 acgacctaaa cagccgggac gacgcgtcta cgcgcggcgt gtcagatgac tggtaacagg
     2401 cctcagtcga gggttagctc cccgcggctg agcgccagcg ctactgcgtg ggcccggttg
     2461 cgcgcgccga gcttctccaa agcgttcttc gtatgcgagc gcgcggtctc ctgagagacg
     2521 ttgagccgct cggccaactc ctgtgtgctc gcccccagtg ccacgagccc catgacctcg
     2581 gcttctcgcg gggtcagcga cacgggctcg cgcgcttggg tagcgtcgcc gccggcccgc
     2641 ccggcgacta gcggcaagag gatcgacaag tgcatgccgg gaaggatgtt ggccgtggcg
     2701 ctgtactcca cggtccgtcg ccgaccgtcc ggcagcagca gttcgaagtc gccggcctgg
     2761 cttccctcgg cgatgaacgc cttccacatc gcttcgacgc ccgtgcgcag ctccggcggc
     2821 gtcagatcgt cgatgcgccg tttcagaatc catccccggg gcgcatcgag cagtcggcag
     2881 gcagcgtggt tcacgtcgcg gtaccgccgt tcgtcgtcgg cgatgagcat cgcattccgg
     2941 ctctgctcga acactgcgcg caggcgttcg gcgcggtccg gcatttgcac cggcacgggc
     3001 cggcccaaag agctgctgcg ccgcgaatcc attggttcga agtacctata agggcgcaca
     3061 acgcaccttg gccaacgcgg agtgtaggac gagccgggaa gcggcgtcgc tgccgggcct
     3121 cccccgtacg ggggagttca cggccagccc gtggcggcga tgctcatcgt ggaggctcag
     3181 acggttcgaa cctgggggcg aggtagggcg tctgacggtc ggattgggcc gcggtgtgag
     3241 ccaagcggcg tttcgaatgg cttagccgga atcactcgag ttaccgagac gagaggactg
     3301 tcgcatgttg gacctgtatg accggcctgc cccgactcgg cccgcacccc ggtcggcgac
     3361 gggcgcagcg gacgtgctgt tcgcccgcta ccggcgtggt gacttcgccg caaggcgcct
     3421 gctgattgag cggttcctgc ccctcgcccg caaccttgca cgccgctacg agggccgcgg
     3481 cgagccgctc gacgatctgg tgcaggtggc gagcgtcggc ctcgtcaagg ccatcgaccg
     3541 cttcgagccc ggacgcgggt tcaaattctc gagctacgcg gtccccacga tgctcggcga
     3601 actgcgacgc cacttccgcg actccggctg ggccgtgcac ctcgcgcgcg acatgcaaga
     3661 gcgcgtgctg aaggtccagg cggccgtcga gcgactgtcc ggccagctcg gccgctcacc
     3721 caacccgcaa caggtcgccg acgagctgaa gatgtcggtc gagcacgtgc tcgaggcgat
     3781 cgaggccaac tcctcctaca aggccgcttc gctcgatgcc ccggcgccct cgtcgctcga
     3841 tgacggcggc aaccaggcgg acatggtcgg cgtcgtcgac gagcggttcg agctgatcga
     3901 ggccgcggcc tcgatcgcac cagcactgcg cgcactgccc gagcgcgagc gaaccatcct
     3961 ctacctgcgc ttcgtcaagg acctcagcca gacccagatt gccaaacaga tcggcgcgtc
     4021 gcaggtgcac gtgtcgcgcc tgatccggca ggcgctcgaa gatgtgcgct cggaaacgga
     4081 gtagcggcgg tgccgtatgt ccgctgcccg cgctgcgacc tcgcggcgtt tagcgcagcc
     4141 cgccactcaa ctcgggacag ttgcgccagc tgcggccaga cgctgccgat cgccggagcc
     4201 cacgggccgg cgacacggaa cccagcggcg gacatggcgg cggagcccag gctggccacg
     4261 cgcgccgagg cggcgtcacc gagttcaggc aagccgcggg tggagtagag gagccgagcc
     4321 ggcgatgcat cctagactcc cgctcccgtg cgcatcttca gcggaatcca gcccaccggg
     4381 cgcaagcacc tcggcaacta catcggggcc atccggcagt acgtggacgg tcaggatcgg
     4441 ggggaggcga tctactgcat cgtcgacctc cacgcgatca ccgtcgcgta cgaccccaag
     4501 gagctccgcg agcgcgtcta cgacacgacg gcgatcctga tcgcggcggg gctcgatccc
     4561 gcccgctgca tcctcttccg ccaggccgac gtgcccgagc acaccgagct ctgttggctg
     4621 ctctcgggcg tcaccgccca cggcgacctc aaccggatga cccagttcaa ggacaagtcc
     4681 gccaggcagc gagagcttgt atcggcgggg ctcttcctct acccagtgct acaggcggcg
     4741 gatgtgctcg cctacaacgc cgacgaggtc ccggtcggcg aagatcagcg ccagcacatc
     4801 gagctgatgc gcgacatcgc ggagcgcttc aacgagcggt tcggcgacac cttcgtggtg
     4861 ccccgggctg tgatccccga ggtgggagcc cggatcatgg atctccagca gccgaccgcc
     4921 aagatgtcca ccacggcgcc cagcgaagcc ggcacgatcc tcgtactcga cgagccgaag
     4981 accatcgaga ggaaggtgaa gagcgcggtc accgactccg gctcggacgt cctacgcagg
     5041 ccgggcaagg aggggatctc gaacctgatc gagatcctgt cggtgatccg cggcgtctca
     5101 cccgacgcgg tcgaggccga gttcgacggc tcgggctatg gcggcttcaa gcaggcagtc
     5161 gctgacgcgg tcgtggaata cctacggccg gtgcgcgagc gctacgagga gatccgcgcg
     5221 gacgagggcg agctcgaacg catcctcgcc gagggcgccg gcaaggcgca cgcgatcgcc
     5281 cacgacatcg tggccgaagc tagcgacaag atgggcgtcg gccctcctcg ctagcctccc
     5341 cgcgatgccc gtcgcctccc tcgacctgga cctggaggtc ttccagggcc cgttcgacct
     5401 gctgctgacg ctcgtgctga aggaggaggt cgacctccgc gaggtggacc tggccgaggt
     5461 ggtgctcgcc tacatcgacc acctcgaggc gcagggcgag ctcgatctcg aggccacgac
     5521 cgagttcctc gtgctgatcg cggcgctgct cgagctgaag tcgcgcctga tcctgccgcg
     5581 cgaggaagag gaggagatcg acctcccgca ggacgaggcg gccgatgagc tgctcgctcg
     5641 catgctcgag tacacccgct accgccgggc ggcgagctgg ctgcacgagc agctgtccga
     5701 cgagtcgggc ttccgctacc gctcggcgcc gcttccgccc gagctgcggc gcgtgtcggt
     5761 ggatctggcg gagaagtcgt acgagcccgt gaagctcgcg gaggcgctcg gcggcctgct
     5821 gcgcaagccg gagcccctca cgcttcgtca catggccacg ccgatcgtgt cggtcgagca
     5881 gcgcctcagc gtgctgcgcg gcctgctcgc tcgctcgcgc agcttcagct tcgacgaggc
     5941 cgtcaggggc gccgaccgcc tcaccgaggc gatgacgctc tgggcgctgc tcgagctcta
     6001 caagcagggc gaggcgcaat ggcagcagga cgagaacttc ggcccgatca gggtgagcgc
     6061 cccgtgaccg agctcgcgcg catcgtcgag gcgctgctct tcctgtcgac
//
LOCUS       DASVXS010000161        20131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_587109, whole genome shotgun sequence.
ACCESSION   DASVXS010000161 DASVXS010000000
VERSION     DASVXS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20131
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587109"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF752_08955"
     CDS             complement(<1..192)
                     /locus_tag="VF752_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855719.1"
                     /translation="MALMEREVGVAVAATAAAMSPKVRRWAREGAVYGLAGAMKAGDV
                     LVATARGAAHGAKEGVSGNH"
     gene            complement(327..680)
                     /locus_tag="VF752_08960"
     CDS             complement(327..680)
                     /locus_tag="VF752_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855720.1"
                     /translation="MGWATGALRPPVGALPEVATRRGFLIHAYSAAQRLASEHHGQGS
                     ASAIVGRAGFHHLLTRRYPAGNRIVRWLHESSSTPLLRMREEEEAMSMIEQLLMLLWG
                     ATPTKRRAPRVMLER"
     gene            813..1037
                     /locus_tag="VF752_08965"
     CDS             813..1037
                     /locus_tag="VF752_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855721.1"
                     /translation="MEILDPRVAVVAGVGAAVIGSESVRRAVGRGAGYAVAGASKVAS
                     PVVDAGREIVGEAREVAGTGKATSRSGTRK"
     gene            complement(1248..2234)
                     /locus_tag="VF752_08970"
     CDS             complement(1248..2234)
                     /locus_tag="VF752_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8855722.1"
                     /translation="MEAASRTAFVTGGSGFIGGRLIERLVSDGVRVRALARSDSSAEA
                     VRQRGAEPVRGDLDNVDAMREGAAGCEWAFHAAANVADSGSREDFERVNVAGTQNALR
                     AAAGAGVRRFVHVGTEAALLAGDPLVNVDETAPLRPDSKSLYPSTKAKAEQAVRAANG
                     DGLETVVVRPRFVWGPGDTTVLPQIAAAVRTGRFAWIGGGRHLTSTTHVDNVVHGLVL
                     GAQRGAAGEAYFVTDGDPVVFREFVTALLDTQGLEPPDRSIPAPLAGALAAVSETLWG
                     TLPLPGGPPLTRMAYWAAGQECTIDISRARSELGYEPVKSLDEGLAELRAPA"
     gene            2334..2705
                     /locus_tag="VF752_08975"
     CDS             2334..2705
                     /locus_tag="VF752_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8855723.1"
                     /translation="MNLPHGQLNFAIRDEVIDAETAVINLSGDVDMHTAPMLKRRMTE
                     LIHEGRRRFIVDLSEVSFIDSTAIGVLVGRRKDLLRRRGTIALVCSDENVLEIFEIAG
                     LPALFEIHDSRERALEARVHA"
     gene            2748..3170
                     /locus_tag="VF752_08980"
     CDS             2748..3170
                     /locus_tag="VF752_08980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8855724.1"
                     /translation="MSPQAISLRQELRELRVPASTEMLFRVRDFAEAAAEAFGLSSRD
                     RYRVKLAASEAAANAIEHGAPDSDGSIKLRVVCEGDSLAMYVSDAGSFATPAVDPDQW
                     SERGRGLAFIAALMDEVEVTREGTGTLVRMAKRLPVAA"
     gene            3222..4850
                     /locus_tag="VF752_08985"
     CDS             3222..4850
                     /locus_tag="VF752_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8855725.1"
                     /translation="MTDAERWFREKYAAAPERDALFTTISGQEIDPLYTSDDLSDPAK
                     IGFPGEYPYTRGVYPSMYRGRLWTMRQFAGFGTAEETNERFRYLLDHGQTGLSTAFDM
                     PSLMGHDSDHPRSLGEVGREGVAVDTLDDMQTLFQGIPMGEVSTSMTINAPAAIMLAF
                     YVVAAEQQGVPPEQLAGTIQTDILKEYIAQKEWCFPIDPAMRLVTDMVEWCSERMPRW
                     HPISISGYHIREAGSTAQQELAFTLKDGFTYVEQAIERGLDVDDFAPRLSFFFNAHLD
                     FFEEIAKYRAARRIWARELKETYGAKNERSLLMRFHSQTAGVSLTAQQPLNNVVRTAI
                     EALAAVLGGTQSLHTNSYDEALALPTEEAVRVALRAQQIIAHETGVTNTIDPLGGAYF
                     IEALTDKIEEAAYDYFRRIDELGGMVEAIKQNFPQREIADASFHYQQEVDSGKRIVVG
                     VNRYRNAEDQPLEILRIDPALERKQIDRVRAVRARREAAAVEGALTSLNEAAAAGANL
                     MEPLLEAARAHATEGEIVESLQRVFGTYTESPVF"
     gene            4956..5273
                     /locus_tag="VF752_08990"
     CDS             4956..5273
                     /locus_tag="VF752_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8855726.1"
                     /translation="MRKLLATLAIVSLIAGVTAAVALGATKTVRVGDNFFSKKSLTIS
                     RGTTVKWSWQSTDNPHNVAGKGFKSTVKSSGSYKHTFRKRGKYIVICQIHPTQMRMTV
                     KVQ"
     gene            5285..5719
                     /locus_tag="VF752_08995"
     CDS             5285..5719
                     /locus_tag="VF752_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX8855727.1"
                     /translation="MEGSAQAATEARQASGRKIRVVVAKPGLDGHDRGAKIIARALRD
                     AGMEVIYTGLHQTPEQIAETVLQEDADAVGLSILSGAHMTLVPRVVELLREQGADDVV
                     ITVGGTIPSEDIPELKKLGVSEVFTPGASTQEIVDFIRGAVP"
     gene            6068..6340
                     /locus_tag="VF752_09000"
     CDS             6068..6340
                     /locus_tag="VF752_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855728.1"
                     /translation="MGLSAETASFRGNDADCLVAASLACPLCLHGDTDWSLGGDVWDT
                     WADCSCPHCGHSRRVYLAGDQALRLSLHVARPLDPEPHPGALDIPL"
     gene            6361..7482
                     /locus_tag="VF752_09005"
     CDS             6361..7482
                     /locus_tag="VF752_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8855729.1"
                     /translation="MVMSPQAQASDARNDALDLLVELLPDVDAAKPTRDFYDRICEAV
                     CRLTSMERAVLFVYDEAVRWVRAAGSHGVDPALIADVHAMLEETPLAQRALSSEEVVV
                     SSAPLDQEVPPRYARMLDVKTLSCTPVSAGGRWFGVILADRGGEPFDVTEEERHTMLS
                     LGKMAALAAGVRMATRQQERARRLAERIELAREVHERAMQRLFGLSLVLGGDDELQKE
                     DRRRCAEELGAALAELRSALERPLAPPERETATTLREELVRLDQRYRDLSITTEWPDG
                     AEVPPDLEPLAQAVLAEALLNAHKHARGSNVRVRVESSADTFAMEVENDGLRESPPGG
                     GLGLRLTAYQAVQHGGVVEFGKAPEDRWRVRLVVPRAAV"
     gene            7479..8081
                     /locus_tag="VF752_09010"
     CDS             7479..8081
                     /locus_tag="VF752_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855730.1"
                     /translation="MTEGRLRVLVVDDHDVVHWGFRLLLSRQPWVERCIAARGADEAL
                     ELTRRYEPHVALVDLFMGDESGADICEAIRAESPSTRVLLISGAGHISAPAAKAAGAS
                     GFISKEWAGDDVARAVRMVAKGMTVFEPAAEAPQNGLTGREREVLDLIASGATNREIA
                     DQLFLSPHTVKEHTSAVYRKLSVRNRAEAVQRAQRLGLIG"
     gene            8183..9031
                     /locus_tag="VF752_09015"
     CDS             8183..9031
                     /locus_tag="VF752_09015"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8855731.1"
                     /translation="MDALLFPGQGSQSSGMRDLACHGRPDLLERAERELGQDPCEAAS
                     SGTYAAQLAIYCASLGGYELLDRPRPDYAAGHSLGEIAALAAADAISAEDGLRLVAAR
                     GRLMEEAARARQGGMVVLRTDSAGATALLNGDGATVANENSPDQTVVSGDEAALLELE
                     WRAGEQGVRAMRLPVAGAFHSPAMEPAVEPFAAELDAVEVREPRFPVISSTSAQPFDG
                     DIRARLAAALTEPVRWTSVLAWLDEQGVRRYVETGPGRVLTGLVRRTLPGVAAVSADP
                     MEMSRA"
     gene            9024..10025
                     /locus_tag="VF752_09020"
     CDS             9024..10025
                     /locus_tag="VF752_09020"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX8855732.1"
                     /translation="MLEAPLASRTRAGVPAAGAAFASVSGVVPDAVVPNARIAHRLGV
                     EERWIETRTGIKERRVVRDGERMLDLAVRAGHAALAGAGIDAVAVDMLLLATTSHDEL
                     VPNGAPQVAAELGAVHAGAIDVGAACTGFVAAVELGAALIESRRAARVLVIGADVMSR
                     HVDPDDRSTAALFGDGAGAALLVSSMPGRIGPTVVGADGEGATLIRAPHPRALIEMQG
                     PETFAQAVDRLCEATVDAAALSGIASLEDIDLFVYHQANARILRAVGERLGLTAERVP
                     DYISSYGNTSAASVPIALAEAERDGLLMPGMRVLIGAFGAGLTWGATVVEWGVPPDA"
     gene            10039..10743
                     /gene="fabG"
                     /locus_tag="VF752_09025"
     CDS             10039..10743
                     /gene="fabG"
                     /locus_tag="VF752_09025"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX8855733.1"
                     /translation="MTGASRGIGAATALALAEDGWPVGVNFRSDSDGAERVVGRISAS
                     GGTAVPLPADVADPGAAEQLFGSLEERFGPVLVLVNNAGVRADGLAPQLGDDEWDLVL
                     DTNLSAAFRLTRRALRRMVTTRFGRVVNVASVVGQRANPGQANYAASKAGLIGFTRTV
                     AAEVARRGVTVNAVAPGFVETEMTNGLLERAVKEIPARRVGTPEEVAACVRFLASEEA
                     GYVTGTTLTVDGGLSA"
     gene            10767..11015
                     /locus_tag="VF752_09030"
     CDS             10767..11015
                     /locus_tag="VF752_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8855734.1"
                     /translation="MPATVTKENVQETIVEAIAKLGPDPSEITPDATFESLEIDSLDL
                     VELAQVIEDEYGVAMKSEDASRLQTVQDAVDLVVERAS"
     gene            11012..12202
                     /locus_tag="VF752_09035"
     CDS             11012..12202
                     /locus_tag="VF752_09035"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX8855735.1"
                     /translation="MTRSVVITGVGAVTPLGVGARALFERWARGESGIEEGLGRCSEF
                     DPTERMSKKEARRADRFTQLAVTAAGEALESAGWNGSPPVEPERVAAIIGTGIGGLES
                     LGTQQDVLRDEGPKRVSPLAVPLMMANAGSAAVAMRHGLTGPSFATASACAAGTDAIG
                     TAMRMIQSGQADAAVAGGAEAALTPLARSAFAAMGATSESGISRPFDARRDGFVMGEG
                     AGVLVLEDAEIARARGAEVLAELRGYGASSDAYHLTAPDPEGSGAARAIEIALEHARV
                     APEDVDYVNAHGTSTPLNDRSETEAIKHALGEHSTEVPISSTKSAIGHLLGAAGAVEA
                     VATVLAIRERLAPPTLGWEAREEGLDLDYVPGKGRELPEGAIAISNSFGFGGHNAVLC
                     IGAA"
     gene            12199..13659
                     /locus_tag="VF752_09040"
     CDS             12199..13659
                     /locus_tag="VF752_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8855736.1"
                     /translation="MTQTAPQLATASRLDPLERLELLCDEGSLRVLRTHVESLRLGPK
                     ARPGDGVVAGAGRVGGRPVYCYAQDNSFAGGSLGAAHADTITEVLRLAGRSRAPVVGF
                     VDSGGARLQEGVAALDGYGRVFREIVALSGVVPQISVICGAAAGGAAYSPALTDFVVM
                     NREAAMFLTGPGVVREAIGEDVTAAELGGAAVHERSGVCELVAESEADAVRAARELLS
                     YLPQSAYDEPPRHPVAVPEDRRDPGRLVPRSSRRVYDVRLVIDELADPGSSLEIAPRF
                     ARNMVTTFARIEGRPVGFIANQPRHLGGTIDWTAARKAARFVRICDAYGLPLVVLVDA
                     PGFLPGSAQEQAGVQREGAKLLHAFAEATVPKLTVVLRKAFGGAYITMNSKALGADLA
                     AAWPDAEIGVMGGQQAASVINRRDIAAADDPDGERAARAAAYAAEHLGAAVAAREGAI
                     DAVIEPADTRSWLAWGLSSFGGRTGGRRARSGNVPL"
     gene            13674..14177
                     /locus_tag="VF752_09045"
     CDS             13674..14177
                     /locus_tag="VF752_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8855737.1"
                     /translation="MPELFSLPFDRLNEGEQFATRGRTVTEADVVAFSSLTGDWHPQH
                     TDAVWAASSVFGERIAHGMLLLSFAVGLLPFDPERVLALRRVEDVVFKRPAFIGDTVH
                     AAGRIDSLSPVDDRAGLVTLAWRMENQHGELLLRARTQVLWAGDGARPSDDSPGLPLT
                     AAGVLPL"
     gene            14177..14986
                     /gene="fabI"
                     /locus_tag="VF752_09050"
     CDS             14177..14986
                     /gene="fabI"
                     /locus_tag="VF752_09050"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX8855738.1"
                     /translation="MLDGKRLLITGVVTRRSIAFSVAEEAQRAGAEVLLTSFGRARRI
                     TERAATRLPDPPDVLELDVNEPSDIAELADELDRRWGSVDGVLHAIAFAPEDALGGRF
                     LETPPESAAAAFQTSAYSLKALACGLLPLLEQSDAGGSVVGLDFDASVAWPAYDWMGV
                     SKAALEAVSRYLARDLGPRGVRVNLISAGPLETVAASGIPGFQELAALWGRGAPLGWD
                     CEDPAPVARAACFLLSDWSRGISGEILHVDGGYHALGAPVTAVTEAATLGD"
     gene            15088..15882
                     /locus_tag="VF752_09055"
     CDS             15088..15882
                     /locus_tag="VF752_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX8855739.1"
                     /translation="MKICVLVKEVPDAAVEKRMDPNTKRLDRSGEKNLNPFDTHAIEA
                     AMQIKEGGAIDVEEIVAVTMGPESAVRALHKAVSLGADRSVHLSDDALAGSDVSATGY
                     ALAQVLKQESPDLVLLGQQSDDGECYTIGAVVADYLQMPSLTQVIKIDVEGNALRCER
                     QAEYGYDTVQVELPAVISVGDAINEPRYPSLKAIMGAKKKPLDAKSTGDVGIETSRVG
                     QDGSRTAVLAINPPPAKEAGEIIEDEDTDETVQKIVSWLEERKLLA"
     gene            15879..16856
                     /locus_tag="VF752_09060"
     CDS             15879..16856
                     /locus_tag="VF752_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX8855740.1"
                     /translation="MSGILTYVLHYDGEFNKNSLGAVSQSAKLAQELGQEAAAIVVGG
                     DDLTDELVGSLGNYGVSKVYRAKGPEGLAQPVIDAMAKVIQDNGYSYALFGGGLLGFE
                     IGAGLAARLNGGVTMEVTEVKPQDGKLVAVRPILQDSALVDVDYVGEPGIIIGRLNAF
                     EISESGGGTAQIEDVDVQLSEWSTKASMVQRGEQRGADVNIEDADILVAGGRGLGKAE
                     GFQLCEALAQALGGAVAATRAVVDAGWYPYAAQIGQTGKTVAPKLYLAAGISGAIQHK
                     VGMQNSENIVAINKDPNAPIFEFSDLGIVGDLNKILPKLTEAVKAKRGG"
     gene            16864..18579
                     /locus_tag="VF752_09065"
     CDS             16864..18579
                     /locus_tag="VF752_09065"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HEX8855741.1"
                     /translation="MGARPADFPPPVKSYEEFVGQPTDPEDERIEVGIVIVGGGPAGL
                     ACAIRLMQLLEDAPQLAESLGEVPVAVIEKGKNTGSHLLSGAMMNPSAMRELFPDVPE
                     EDWPTYGPVSKDAVYLMTDKTATRLPTPPPFRNHGNHVTSVAQLGRWLGEKAEEAGVY
                     VLTESAGYKLLVDDGQVVGVRTGDKGRGRDGEPLPNFEPGSDVIAKATVLAEGTQGHL
                     TGAAIGRFGLGADDPQQWEIGVKEVWEVTKPLDRVIHTMGWPLKLSSRYGEFGGSFCY
                     PMGDDKVSIGFVVGLDYKDANFSVHDTLQLIKTHPLFKKVLEGGKRVGWGAKTLPSGG
                     YWAMPSQLWAPGLVLAGDGAGMVNVPKLKGVHYAMHAGIYAAEAIFDGLKKGDTSDLS
                     GYEDRVRDSVIEKDLYRSRNMRQPFTRGFFVGGAIANVLEASGGRLPPGHFATHDDAE
                     VEVFQGKKRDYPKPDGKLTFDKLSSVFATGNATRDDAPNHVRVQRNVPIEIALMWKNM
                     CPAYVYELADEVQEQLDNGGIPDGTLVEVHLTPSNCVQCGAITAKGGRLTPPEGGDGP
                     NYQLT"
     gene            18633..19520
                     /locus_tag="VF752_09070"
     CDS             18633..19520
                     /locus_tag="VF752_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855742.1"
                     /translation="MDLAVGIAASVVAALIFTGSVTVQALEARAVSHEHAMRATLLRS
                     LAARRRWLGAISLLVLGWIFYVFALTLAPLTVVQPVRAVGLLVLLWVGVRVLGERAGR
                     RELIGVLLIVGGVVALGAAAPEHSSNHASAGELVLALGLLGAAALAPFAVRGGDRGLS
                     LLVVFAAGLAFSWVDFASKLMADALHDHHFLGAWAWATAIGVFAVVGLLAENSSFQRR
                     PATQVVPIVFALQMALPAALAALVGGENWGDTPLGGAVIGVALVLVTVGTLVLASSHA
                     VADVVAAAEGAEEAEPAAA"
     gene            19774..>20131
                     /locus_tag="VF752_09075"
     CDS             19774..>20131
                     /locus_tag="VF752_09075"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8855743.1"
                     /translation="MPRTRQPIRRASVVGAGSFGTAVAALLVRAGIRTTLLCRTQEQA
                     DELSARAENERYLKGVKLPDRLKVRVLGGREDQFGRADLVLLAVPSAGLRAAIDELLR
                     QGVPRTTAVVSLAKGLV"
BASE COUNT         3022 a         6981 c         7231 g         2897 t
ORIGIN      
        1 gtggttaccc gagactccct ccttagcccc gtgtgcggca ccacgcgccg tagcaaccag
       61 cacgtcaccc gccttcatcg cgccggccag gccgtagacc gctccctcgc gcgcccaccg
      121 tcggaccttg ggagacatcg cggccgcggt cgctgccacc gccactccga cctctcgctc
      181 catgagtgcc atgccccggc tcctccttcc gctggacgat cagtcaggac ggatcgaggt
      241 ccggctcgtc cggcggaaca ccggatctcg caggcagagt atccgagcgc gcccgggcga
      301 aaccagccct cgcgaagaag gacagctcag cgctcgagca tcacgcgggg tgcgcgcctc
      361 ttggtgggcg tcgctcccca gagcaacatc aacagctgct cgatcatcga cattgcttcc
      421 tcctcctcgc gcatccgcag aagcggcgtg cttgaagatt cgtgaagcca tcggacgatc
      481 ctgttgccag ccggataccg gcgggttaag agatggtgaa aaccggctcg cccgacgatg
      541 gcactcgcgc tgccctggcc gtgatgctcg gacgccaatc gctgtgcagc cgagtaggcg
      601 tggatgagga atccgcgccg tgtggcaact tcgggcagcg ccccgacggg cggccgcaag
      661 gctccggtgg cccagcccat ggctccctgg taccggggac cgtggtttcg ttgtcattcg
      721 aatcggtacc tctcggctgt ggcgtcgcac ggccgcgtgc tcgcgcgtcg ggacgcccgc
      781 cgtctggcca ttgagcagtg aagggaggaa ggatggaaat cctcgacccc agggttgcgg
      841 tggtcgccgg tgtcggtgcc gcggtgatcg gatcagagag tgtgcgccgg gcggtcggcc
      901 gtggcgcagg ctacgcggtc gcgggcgcgt cgaaggtcgc gtcgcctgtg gtcgacgcgg
      961 gtcgcgagat cgtcggagaa gcccgcgagg ttgccggcac tggcaaggcc acgtcgcgga
     1021 gcggcacacg caagtagcga ctcgagtcgc cgtgaagggc cgccggcctt tgttgagggt
     1081 tccgtctcgc cggcggcccc gcacgcgctt cgggcgtgtg cctggccggt cgggttcgcc
     1141 tgcgaccggc ccgccacgcc ccggaggccc agtccaagcc aaccggtgca tgagttgttg
     1201 caccgccggg cgcgggtgta accgcggatg gccggatgta cgagcgatca ggcgggggcg
     1261 cggagctcgg cgagcccctc gtctagggac ttcaccggct cgtagccgag ctcagaccgc
     1321 gcgcgcgaga tgtcgatcgt gcactcctgt ccggctgccc agtaggccat ccgagtgagc
     1381 ggcggcccgc ccggcagcgg cagcgttccc cagagggtct cggaaacggc cgcgagcgcg
     1441 ccggcgagcg gcgccggaat gctgcggtcg ggcggctcga gcccctgtgt gtcgagcagc
     1501 gcagtcacga actcgcgaaa gacgaccggg tcgccgtccg tgacgaaata cgcctcgccg
     1561 gctgccccac gctgggcacc gagcacgagg ccgtgcacca cgttgtccac gtgagtggtc
     1621 gacgtcaggt gccggccccc gccgatccac gcgaagcggc ctgtgcggac ggcggcggcg
     1681 atctgcggga ggacggtcgt atcgccgggc ccccacacga atcgcggccg caccaccacc
     1741 gtctcgaggc cgtcgccgtt ggcggctcgg accgcctgct cggccttggc cttggtggag
     1801 ggatagagcg acttcgagtc aggccggagc ggcgcggtct cgtccacgtt cacgagcggg
     1861 tcgccggcga gcagcgccgc ctcggtgccc acgtgcacga agcggcggac gccggcgccc
     1921 gcggccgcgc gcagcgcgtt ctgggtgccg gccacgttca cgcgctcgaa gtcctcgcgg
     1981 ctgcccgaat cggccacgtt cgccgccgca tgaaaggccc actcgcagcc cgcagcgccc
     2041 tcacgcatcg cgtcgacgtt gtcgaggtcg ccacgcaccg gctcggctcc ccgctgcctg
     2101 acggcctcgg ctgagctgtc ggaacgggcg agggcgcgca cccgcacgcc gtcgctcacg
     2161 aggcgctcga tcagccgccc gccaatgaag ccggagcctc cggtcacgaa cgcggtgcgg
     2221 cttgcggcct ccatcgcggc ggatcgtaca tccgtccaag tgacagttgg aacaggtgtg
     2281 cggggggaac atcgcgaaag acgtcccacc agcccctcaa accggaggac tcgttgaacc
     2341 ttccacacgg ccagctcaac ttcgccatcc gcgacgaggt gatcgacgcg gaaaccgctg
     2401 tgatcaacct ctccggcgac gtggacatgc acacggcgcc gatgctcaag cgacgcatga
     2461 ccgagctcat ccacgaaggc cgccgccgct tcatcgtgga cctctcggag gtgtcgttca
     2521 tcgactcgac ggccatcggc gtcctggtcg gcaggcgcaa ggacctgctc cgccgccgcg
     2581 gcaccatcgc gctcgtctgc tcggacgaga acgtgctcga gatcttcgag atcgcgggtc
     2641 tacctgcgct ctttgagatc cacgactcgc gcgagcgcgc gctcgaggcg cgcgtgcacg
     2701 cctagccgca gacccaggac ccgagaaacg cattaggggg gagacccgtg agcccacagg
     2761 ccatcagcct gcgccaggag ctgcgcgagc tgcgcgtccc ggcgagcacg gagatgttgt
     2821 tccgcgtccg cgacttcgcg gaggcggccg ccgaggcgtt cggcctctcg tcacgcgatc
     2881 gctaccgcgt gaagctggca gccagcgaag ctgcggccaa cgcgatcgag cacggagcgc
     2941 ccgacagcga cggctcgatc aagctgcgcg tcgtgtgcga gggcgactcg ctcgctatgt
     3001 acgtgtccga tgccggcagc ttcgcgacgc cggccgtcga tccagaccag tggtccgagc
     3061 gcggccgcgg actcgcgttc atcgccgcgc tgatggacga ggtggaggtc accagggagg
     3121 ggacgggcac gctcgtacgc atggccaagc gccttccggt cgccgcgtag cacgccacct
     3181 ccgtcactag actgcggctc atggcggccc ctgcacgccc catgaccgac gccgaacgct
     3241 ggttccgcga gaagtacgcg gccgcgccgg agcgcgacgc gctgttcacg acgatctccg
     3301 ggcaggagat cgacccgctg tacacgtccg acgacctgtc ggatcccgcc aagatcggct
     3361 tcccgggcga gtatccctac acgcgcggcg tgtacccgtc gatgtaccgc gggcgactct
     3421 ggacgatgcg ccagttcgcg ggcttcggca cggccgagga gacgaacgag cgcttccgct
     3481 acctgctcga ccacgggcag acggggctct ccacggcgtt cgacatgccg tcgttgatgg
     3541 ggcacgactc cgaccatccg cgctcgctgg gcgaggtggg ccgcgagggc gtcgccgtcg
     3601 acacgctcga cgacatgcag acgcttttcc agggcattcc gatgggcgag gtctccacct
     3661 cgatgaccat caacgccccg gccgcgatca tgctcgcctt ctacgtcgtc gcggcggagc
     3721 agcagggcgt gccgccggag cagctcgccg gcacgatcca gaccgacatc ctcaaggagt
     3781 acatcgccca gaaggagtgg tgcttcccga tcgacccggc gatgcgcctc gtgaccgaca
     3841 tggtcgagtg gtgctccgag cgcatgccgc gctggcaccc gatctcgatc agcggctacc
     3901 acatccgcga ggccggctcg acggcgcagc aggagctcgc cttcacgctg aaggacggct
     3961 tcacgtacgt ggagcaggcg atcgagcgcg gcctcgacgt ggacgacttc gcgccgcggc
     4021 tgagcttctt cttcaacgcc cacctcgact tcttcgagga gatcgccaag taccgcgccg
     4081 cccgccggat ctgggcgcgc gagctgaagg agacctacgg cgcgaagaac gagcggtcgc
     4141 tgctgatgcg cttccactcg cagacagcgg gcgtgtcgct cacggcgcag cagccgctca
     4201 acaacgtcgt ccgcacggcg atcgaggcgc tagcagcggt gctcggcggc acgcagtcgc
     4261 tgcacacgaa ctcatacgac gaggcgctcg ccttgccaac cgaggaggcg gtgcgcgtgg
     4321 cgctccgcgc gcagcagatc atcgcccacg agaccggtgt gacgaacacc atcgacccgc
     4381 tcggtggcgc ctatttcatc gaggcgctta ccgacaagat tgaagaagcc gcgtacgact
     4441 acttccgccg catcgacgag ctcggcggca tggtcgaggc gatcaagcag aacttcccgc
     4501 agcgcgagat cgccgacgct tccttccact accagcagga ggtcgactcc ggcaagcgca
     4561 tcgtggtcgg cgtgaaccgc taccgcaacg ccgaggacca gccgctcgag atcctgcgca
     4621 tcgaccccgc gctcgagcgc aagcagatcg accgcgtgcg cgccgtgcgt gcccgccgcg
     4681 aggccgcagc tgtcgagggc gcactgacga gcctcaacga ggccgccgcg gccggcgcga
     4741 acttgatgga gccgctgctc gaggcggcac gcgcgcacgc caccgagggc gagatcgtcg
     4801 agtcgctgca gagggtgttc ggcacctaca cagagtcacc ggtcttctag ccggtgtagc
     4861 gtgttgtcac agggaggccc cgcgccgggc gggacgaact actggcccga gcgcgtcagc
     4921 tcaggggaaa gcactcacgg aaagaggact ggttcatgcg caagctccta gccacgctcg
     4981 cgatcgtttc gctgatcgca ggcgtgacgg cggccgtggc tctcggcgcc accaagacgg
     5041 tccgggtcgg cgacaacttc ttcagcaaga agtcgctcac catcagccgg ggcaccacgg
     5101 tgaagtggag ctggcagagc acagacaacc cccacaacgt ggccgggaag ggcttcaagt
     5161 caacggtcaa gagttccggc agctacaagc acaccttccg caagcgcggg aagtacatcg
     5221 tcatctgcca gatccatccg acgcagatgc gcatgaccgt caaggtccag tagctttcgc
     5281 cgacgtggaa ggcagcgctc aagcggctac ggaggcccgg caggcttccg ggcgcaagat
     5341 cagggttgtc gtcgcgaagc ccggcctcga cgggcacgat cgcggcgcga agatcatcgc
     5401 tcgcgctctg cgcgacgcgg gcatggaggt catctacacc ggcctgcacc agacgccgga
     5461 gcagatcgcg gagacggtgc tccaggaaga cgcggacgcg gtcggcctct cgatcctctc
     5521 gggcgcgcac atgacgctcg tgccgcgcgt ggtggagctg ctcagggaac agggggccga
     5581 cgacgtggtg atcacggtcg gcgggacgat cccgtccgag gacatccctg agctgaagaa
     5641 gctcggcgtc tcggaggtct tcacgccggg cgcgtccacg caggagatcg tcgacttcat
     5701 ccgcggcgcg gtcccctagc cggcgaggac ccagccggcg ctcggttgcg agctgtggcg
     5761 gccggccgtg gctggaccgg agctccggct cccttagccg gaaacagcgg caataactgt
     5821 tggcctggag cgggttcgcc ggccccggac gaacgagatt gattttgagc ccctatatgg
     5881 ggctcaactt aagggtggtt ctgtggacct ttcgcagcac cgccgggggc atcccgttat
     5941 tgctgctgta aacgcccggc acaaccgcac ggccccgcgg ctactcgccg gccaagcacc
     6001 cccctgatcg gggggtttac ggcgcgaatc cctgttacct ccgggcgtcc ggggtgatct
     6061 tccgtgcgtg ggcctatcgg cagagacggc aagcttccgc ggcaatgacg ccgactgcct
     6121 cgtggcggcg tcgctcgcat gcccgctgtg cctgcacgga gacacagact ggagcctcgg
     6181 cggcgacgtc tgggacacgt gggcggactg ctcctgcccc cactgcgggc actcgcgcag
     6241 ggtgtacctc gcgggtgacc aggcgctccg gctgtcgctg cacgtggcgc gcccgctcga
     6301 ccccgagccc cacccgggcg cgctcgatat tccgctataa agccatctag ggcgggggcc
     6361 atggtgatga gccctcaagc gcaagcgagt gacgcacgca acgacgcgct cgacctgctg
     6421 gtcgagttgc tgccggacgt cgatgcggcg aagccgacgc gcgacttcta tgaccgcatc
     6481 tgtgaggcag tgtgccggct cacgtcgatg gagcgcgcgg tcctgttcgt ctacgacgag
     6541 gcagtgcgct gggtgcgcgc ggccggctcg cacggcgtag acccggcgct gatcgcggac
     6601 gtgcacgcga tgctcgagga gacgccgctc gcgcagcggg cgctctcgag cgaggaggtg
     6661 gtggtgtcga gcgcgccgct cgaccaggag gtgccgcctc gctacgcgcg catgctcgac
     6721 gtgaagacgc tgagctgcac gccggtgtcc gcggggggac gctggttcgg cgtgatcctc
     6781 gccgaccgcg gcggcgagcc cttcgacgtg accgaagagg agcgccacac gatgctctcc
     6841 ctcggcaaga tggcggcgct cgccgctggc gtgcgcatgg ccacgcgcca gcaggagcgc
     6901 gcccgccggc ttgcggagcg catcgagctg gcgcgcgagg tgcacgagcg cgcgatgcag
     6961 cggctgttcg gcctctcgct cgtgctgggc ggcgacgacg agctccaaaa agaggatcgg
     7021 cggcgctgcg ctgaggagct gggcgccgcg ctcgcggagt tgcgaagcgc gctcgagcgc
     7081 ccgctcgcgc ctccggagcg cgagactgcg acaacgctcc gcgaggagct cgtacggctg
     7141 gaccagcgct accgcgacct ctcgatcacc acggaatggc cggacggggc ggaggtcccg
     7201 cccgacctcg agccgctcgc acaggcggtg ctcgccgagg cgctactgaa cgcgcacaag
     7261 cacgctcgcg gctcgaacgt gcgggtccgc gttgagtcga gcgccgacac gttcgccatg
     7321 gaggtcgaga acgacggcct gcgagaaagc ccgcccggcg gagggctggg cctgcggctg
     7381 acggcctacc aggcggtcca gcacggaggc gtcgtcgagt tcggcaaggc gccggaggac
     7441 agatggcgag tgcggctcgt ggtcccgagg gcggccgtat gaccgagggc cggctgcgcg
     7501 tgctggtggt cgacgaccac gacgtcgtgc actggggctt ccggctcctc ctctcgcggc
     7561 agccctgggt cgagcgttgc atcgccgctc gtggcgccga cgaggcgctg gaactcaccc
     7621 gccgctacga gccacacgtc gcgctcgtcg acctgttcat gggcgacgag tcgggcgccg
     7681 acatctgcga ggcgatccgc gcggaatcgc ctagtacgcg cgtgctgctt atctcgggcg
     7741 cgggccatat ctcggcgcct gccgcaaagg cggccggcgc ttccggtttc atctcgaagg
     7801 agtgggcggg cgacgacgtg gcgcgcgcgg tgcgcatggt cgcgaagggc atgaccgtct
     7861 tcgagccggc agccgaagcg cctcagaacg gcctcaccgg ccgcgagcgg gaggtactcg
     7921 acctgatcgc ctccggcgcg acgaaccgcg agatcgcgga tcagctcttc ctctcgccgc
     7981 acacggtgaa ggagcacacg agcgcggtct accggaagct gagcgtgcgc aatcgcgccg
     8041 aggcggtgca gcgtgcgcag cggctcggcc tgatcggctg agcgccgccc accccccggc
     8101 catccccgga tagccccccg tccgggggga tggtcgggcc gaggggttca gcccgcgtgc
     8161 ggttccggcc acgctctgca tcgtggacgc actgctgttc ccgggacagg gcagccagtc
     8221 gtcgggcatg cgtgacctcg cgtgtcacgg acggccggac ctgctggagc gcgccgagcg
     8281 cgagctcggc caggacccat gcgaggccgc ttccagcgga acctacgccg ctcagctcgc
     8341 catctactgc gcgagccttg gcggatacga gcttctcgac cgcccgcgac ccgactacgc
     8401 cgccggccac tcgctgggtg agatcgccgc gttggccgcc gccgacgcga tctcagccga
     8461 ggacggcctg cgcttggtcg ccgctcgcgg gcggttgatg gaggaggcag cgcgcgcccg
     8521 ccagggcggc atggtggtgc tgcgcaccga cagcgccggc gcgacagcgc tgctgaacgg
     8581 cgacggcgcc acggtcgcga acgagaactc gccggaccag accgtcgtct cgggcgacga
     8641 ggcggcgctg ctcgagctcg aatggcgcgc cggtgagcag ggcgtacgcg cgatgcggct
     8701 gccggtcgcg ggcgccttcc actcgcccgc gatggagccg gcggtcgagc cgttcgctgc
     8761 cgagctcgac gccgtggagg tgcgcgagcc gcgcttcccg gtcatctcgt cgacgtcggc
     8821 acaaccgttc gacggtgaca tccgcgcgcg gctggccgcc gccctcaccg agccggtccg
     8881 ctggacctcg gtgctcgcct ggctcgacga gcagggcgta cggcgctacg tcgaaactgg
     8941 gccggggcgg gtgctgacag ggctcgtgcg ccgcacgttg cccggcgtcg ccgcggtgag
     9001 cgcggacccg atggagatgt cccgtgcttg aggccccact cgccagccgc acgcgcgcag
     9061 gcgtgcccgc tgcgggcgcc gcgttcgcgt ccgtctccgg cgtcgttccc gacgcagtcg
     9121 taccgaacgc tcgcatcgcg caccgcctgg gcgttgagga gcgttggatc gagacgcgca
     9181 ccggcatcaa ggagcgccgc gtcgtccgcg acggcgagcg catgctcgac ctcgcggtgc
     9241 gcgccggtca cgccgctctc gcgggcgccg gcatcgacgc agtcgctgtc gacatgctgc
     9301 tgctcgccac aacgagccac gacgagctcg tgcccaacgg cgcgccccaa gtggccgccg
     9361 agctcggcgc cgtgcacgcc ggagcgatcg acgtcggcgc cgcctgcaca ggcttcgtcg
     9421 ccgccgtcga gcttggggcg gctctgatcg aatctcgccg cgctgctcgc gtgctggtga
     9481 tcggcgccga cgtgatgagc cggcatgtgg atcccgacga tcgctccacg gctgccctgt
     9541 tcggcgatgg ggccggcgct gcgctgctcg tctcctccat gccggggcgg atcgggccga
     9601 cggtcgtcgg cgcggacggc gagggcgcca ccctgatccg cgcgccgcac ccgcgcgcgc
     9661 tgatcgagat gcagggcccc gagactttcg ctcaggccgt ggaccgcctc tgcgaagcca
     9721 cggtcgacgc cgccgctctc tcgggcattg cctctctcga ggacatcgac ctgttcgtgt
     9781 accaccaggc gaacgcgcgg atactgcgcg ccgttggtga gcggctcgga ctgaccgccg
     9841 agcgcgtgcc cgactacatc agcagctacg gcaacacgtc ggccgcgtcg gtcccgatag
     9901 ctcttgcgga ggccgagcgg gacggactgc tcatgcccgg catgcgcgtc ctgatcggcg
     9961 cgttcggcgc gggcctcacc tggggcgcca ccgtcgtgga gtggggtgta ccgcctgatg
    10021 cgtgagggat gcgcgctggt gactggagcc tcgcgcggga tcggcgccgc gaccgccctt
    10081 gcgctcgccg aagacgggtg gcccgtagga gtgaacttcc gcagcgactc cgacggcgcg
    10141 gagcgcgtgg tgggacggat atccgcgtcg ggcgggaccg cagtgccgct gccggccgac
    10201 gtggcggacc ccggcgccgc cgagcagctc ttcggcagcc tcgaggagcg cttcggcccc
    10261 gtgctcgtgc tcgtgaacaa tgccggcgtg cgcgccgacg ggctggcgcc gcagctgggc
    10321 gacgacgagt gggacctcgt gctcgacacg aacctctcgg ccgccttccg gctcacgcgc
    10381 cgggcgctgc ggcgcatggt caccacacgc ttcggccggg tggtcaacgt cgcgtcggtg
    10441 gtgggccagc gggcgaaccc cggccaggcc aactacgcgg catcgaaggc cgggctgatc
    10501 ggcttcacgc gcaccgtggc cgcggaggtg gcgcgccgcg gcgtgaccgt gaacgccgtc
    10561 gcgccgggct tcgtggagac cgagatgacc aatggcctgc tggagcgggc cgtgaaggag
    10621 atcccggcgc ggcgcgtcgg cacccccgag gaggtcgcgg cgtgcgtgcg cttcctggcc
    10681 tcggaggagg ccggatacgt gaccgggacc acgctgaccg tcgacggcgg actcagcgcg
    10741 tgacaacaac gacgaaggag aggtagatgc cggcaacagt cacgaaggag aacgtgcagg
    10801 agacgatcgt cgaggcgatc gcgaagctcg ggcccgaccc gagcgagatc acgcccgacg
    10861 ccacgttcga gtcgctcgag atcgactcgc tcgatctcgt cgagctcgcg caggtgatcg
    10921 aggacgagta cggcgtggcg atgaagagcg aggacgcgtc gcgactccag accgtgcagg
    10981 acgccgtgga cctggtggtg gagagggcat cgtgacccgc agtgtcgtca tcaccggcgt
    11041 gggcgccgtc acgcccctcg gggtgggggc gcgtgcgctg ttcgagcgct gggcgcgcgg
    11101 cgagagcggt atcgaggagg gcctcggccg ctgctccgaa ttcgacccga ctgagcgcat
    11161 gtcgaagaag gaggcgcgcc gcgccgaccg cttcacccag ctggcggtca ccgctgccgg
    11221 cgaggcgctc gagagcgcgg gatggaacgg ctcgcctccg gtcgagcccg agcgcgtcgc
    11281 ggccatcatc ggcaccggca tcggcggcct cgagtcgctc ggcacgcagc aagacgtgct
    11341 gcgagacgag ggcccgaagc gggtgtcgcc cctggccgtg ccgctgatga tggcgaacgc
    11401 cgggtcggcc gctgtcgcga tgaggcacgg cctgaccggc ccttctttcg cgaccgcctc
    11461 ggcgtgcgcg gctggcaccg acgcgatcgg taccgccatg cggatgatcc agtccgggca
    11521 ggccgacgcc gccgtcgccg gcggcgccga ggccgcgctc acgccgctcg cgcgcagcgc
    11581 attcgcggcg atgggcgcca cgtccgagag cgggatctcg cgcccgttcg acgcccgccg
    11641 cgacggcttc gtgatgggcg agggcgcggg cgtgctggtg ctcgaggacg ccgagatcgc
    11701 gcgggcgcgc ggcgctgagg tgctcgccga gctgcgcggc tacggcgcga gctccgacgc
    11761 ctaccacttg acggcccccg accccgaggg ctcgggcgcg gcgcgcgcaa tcgagatcgc
    11821 cctcgagcac gcgcgcgtcg cacccgaaga cgtcgactac gtgaacgcac acggcacctc
    11881 cacgccgctg aacgaccgct ccgagaccga ggcgatcaag cacgcgctcg gggagcactc
    11941 gacggaggta ccgatctcct ccacgaagtc ggcgatcggc cacctgctcg gcgctgccgg
    12001 cgcggtcgag gcggttgcca cggtgctcgc aatccgcgag cgcctcgcgc cgcccacgct
    12061 cggctgggag gcgcgcgagg agggcctcga cctcgactac gtgcccggca aggggcgcga
    12121 gctgcccgag ggcgcgatcg cgatctcgaa ctcgttcggc ttcggcggac acaacgccgt
    12181 gctgtgcata ggggcggcat gacccagacc gcaccgcaac tcgcgaccgc ctctcgcctc
    12241 gatccgctcg agcgcctcga gctgctctgc gacgagggct cgctgcgcgt gctccgcacg
    12301 cacgtcgagt cgctgagact cggccccaag gcgcgcccgg gggacggtgt cgtcgccggc
    12361 gcggggcgcg tggggggcag gccggtctat tgctacgcgc aggacaactc gttcgccggc
    12421 ggctcgctcg gcgccgcgca tgccgacacc atcaccgagg ttctccgtct cgccggccgc
    12481 tcgcgtgcgc cggtcgtcgg cttcgtggac tccggcggcg ctcgcctcca ggagggtgtg
    12541 gcagcgctcg acggctacgg gcgggtgttc cgcgagatcg tcgcgctttc cggcgtggtc
    12601 ccgcagatct cggtgatctg cggcgcggcg gccggaggtg ccgcctactc gcccgcgctc
    12661 accgacttcg tggtcatgaa ccgtgaggcg gcgatgttcc tcaccgggcc gggcgtcgtt
    12721 cgcgaggcga tcggcgagga tgtcaccgcc gcggagctcg gtggcgccgc cgtgcacgag
    12781 cgaagcggcg tctgcgagct cgtcgccgag tccgaggcgg acgcggtgcg cgcggcgcgc
    12841 gagctgctct cctacctgcc gcagagcgct tacgacgagc cgccgcggca cccggtcgcg
    12901 gtgccggaag atcgccgcga cccagggcgc ctcgtgccgc gttcttcgcg ccgcgtgtac
    12961 gacgtgcggc tcgtgatcga cgagctggcc gacccaggct cgtcgctaga gatcgccccg
    13021 cgcttcgcac ggaacatggt caccaccttc gcccgcatcg aggggcgccc ggtcggcttc
    13081 atcgccaacc agccacgcca cctcggcggc acgatcgact ggaccgccgc ccgcaaggcc
    13141 gcgcgcttcg tgcgtatctg cgacgcatac gggctgccac tcgtggtgct ggtcgacgcg
    13201 cccggcttcc tgcccggctc ggcgcaggag caagcgggcg tgcagcgcga gggcgcgaag
    13261 ctgcttcacg cgttcgcgga ggcgacggtg ccaaagctca ccgtggtgct gcgcaaggcg
    13321 ttcggagggg cgtacatcac gatgaactcg aaggcgctcg gtgccgatct cgccgccgcc
    13381 tggccggacg cggagatcgg cgtgatgggc ggccagcagg ccgcgtcggt catcaaccgg
    13441 cgcgatatcg cggccgcaga cgacccggac ggcgagcgcg ccgcccgcgc cgccgcttac
    13501 gccgccgagc acctcggcgc ggcggtcgcc gcgcgcgagg gcgcgatcga cgccgtgatc
    13561 gagcccgccg acacccgctc gtggctcgcc tggggcctga gctccttcgg cggtcgcacc
    13621 gggggcaggc gcgcgcgctc cgggaacgtc ccgctctgac aaaggaggac cgcatgcccg
    13681 aactcttctc actcccgttc gaccggctca acgaggggga gcagttcgcc acgcgcgggc
    13741 gaacggtcac cgaggccgac gtggtcgcgt tctcgtcgct caccggcgac tggcacccgc
    13801 agcacaccga cgcggtgtgg gcggcgtcga gcgtcttcgg cgagcgcatc gcccacggga
    13861 tgctgctgct gtcgttcgcg gtggggctgc tgccgttcga tcccgagcgc gtgctcgcgc
    13921 tgcgccgagt ggaggacgtc gtcttcaagc gcccggcatt catcggcgac acggtgcacg
    13981 cggccggccg catcgactcg ctgtccccgg tggacgatcg cgccggcctc gtcacgctcg
    14041 cctggcgcat ggagaaccag cacggcgagc tgctgttgcg cgcacgcacc caggtgctct
    14101 gggcgggcga cggcgcccgg ccttccgatg acagcccggg cctgccgctc accgcggcgg
    14161 gcgtgcttcc gctgtgatgc tcgacggcaa gcgactgctg atcaccggcg tcgtcacgcg
    14221 ccggagcatc gccttctccg tggcggagga ggcgcagcgc gccggtgcgg aggtactgct
    14281 gacgagcttc ggccgcgcgc gccggatcac cgagcgcgcg gcgactcgcc ttccggaccc
    14341 gcccgacgtg ctggagctcg acgtgaacga gcccagcgac atcgcggagc tcgcggacga
    14401 gctcgaccgg cggtggggga gcgtcgacgg cgtccttcac gcgatcgcct tcgcgcccga
    14461 ggacgcgctc ggcgggcgct tcctcgagac accgcccgag agtgccgcgg ccgccttcca
    14521 gacgagcgcc tactcgctca aggcgctcgc ctgcggcctg ctcccgctgc tcgagcagag
    14581 cgacgccggc gggagcgtcg tcggcctcga cttcgacgcc tccgtcgctt ggccggccta
    14641 cgactggatg ggcgtttcga aggccgcgct cgaggcggtg tcgcgatacc tcgcgcgcga
    14701 cctcggcccg cgcggcgtgc gcgtgaacct catttcggcg ggtccgctcg agaccgtcgc
    14761 cgcgagcggg atccccggat tccaagagct cgcggcgctt tgggggcgcg gcgcaccgct
    14821 cggctgggac tgcgaagatc ccgcgccggt cgcccgggcg gcgtgcttcc tcctatcgga
    14881 ctggtcccgc ggcataagcg gcgagatcct gcacgtggac ggcgggtatc acgcgctggg
    14941 cgcccctgtg acagccgtca cagaagcggc gacgctgggc gactgactcg tcagtcaacc
    15001 gggtagaatg ccgcccggcg ccacccgcgg aggcggatgc tggccgcctg gggagagccc
    15061 gcgtagtacc gaacgaagga gcctgtcttg aagatttgcg tcctggtcaa agaggtgccg
    15121 gacgccgcag tcgagaagcg catggacccg aacaccaagc gcctcgaccg cagtggcgag
    15181 aaaaacctca atccctttga cacgcacgcc atcgaggccg ccatgcagat caaggaaggc
    15241 ggcgccatcg atgtggaaga gatcgttgcc gtgaccatgg ggcccgagag cgccgtccgc
    15301 gcgctccaca aggccgtctc gctcggcgcc gaccgctcgg tgcacctcag cgacgacgcc
    15361 ttggccggct ccgacgtgag cgcgacgggc tacgcgctcg cgcaggtgct caagcaggaa
    15421 agcccggacc tcgtcctact cgggcagcag tcggacgacg gcgagtgcta cacgatcggc
    15481 gccgtcgtgg cggactacct gcagatgccg tcgctcaccc aggtcatcaa gatcgacgtg
    15541 gaaggcaacg cactccgctg cgagcgccag gccgagtatg gctacgacac cgtccaggtg
    15601 gaattgcccg cggtgatctc ggtcggcgac gccatcaacg agccgcgcta cccgtcgctc
    15661 aaggcgatca tgggcgcgaa gaagaagccg ctcgacgcaa agtcgaccgg cgacgtgggc
    15721 atcgagacga gcagggtcgg ccaggacggg tcacgcaccg ccgtcctcgc gatcaacccg
    15781 ccgcccgcga aggaggccgg cgagatcatc gaggacgagg acaccgacga gacggtccag
    15841 aagatcgtct cctggctcga ggaaaggaag ctgctggcat gagcgggatc ctcacttacg
    15901 ttcttcacta cgacggcgag ttcaacaaga actcgctcgg agccgtctcc cagagcgcga
    15961 agctcgcgca ggagctcggc caggaggccg ccgcgatcgt cgtcggcggc gacgacctga
    16021 ccgacgagct cgtcggttcg ctcggcaatt acggcgtgtc gaaggtctac cgcgcgaagg
    16081 ggcccgaagg cctcgcgcag ccggtcatcg acgcgatggc caaggtcatc caggacaacg
    16141 gctacagcta cgcgctgttc ggcggcggcc tgctcgggtt cgagatcggc gccggcctcg
    16201 ccgcccgcct gaacggcggc gtgacgatgg aggtcacgga ggtcaagccg caggacggca
    16261 agctcgtcgc ggtccggccg atcctccagg actccgcgct cgtggacgtc gactacgtgg
    16321 gcgagccggg catcatcatc gggcgcctga acgcgttcga gatctccgag tcgggcggtg
    16381 gcacggctca gatcgaggac gtggacgtgc agctgtccga gtggtccacc aaggcgtcga
    16441 tggtccagcg cggcgagcag cgcggcgcgg acgtgaacat cgaggacgcc gacatcctcg
    16501 tcgcgggcgg ccgcggtctc ggcaaggccg agggcttcca gctctgcgag gccctggcgc
    16561 aggcgctcgg cggcgcggtc gccgccaccc gcgcggtggt cgacgccggc tggtatccgt
    16621 acgccgcgca gatcggccag accggcaaga cggtcgcgcc gaagctgtac ctcgcggccg
    16681 gcatctcggg cgcgatccag cacaaggtcg gcatgcagaa ctccgagaac atcgtggcga
    16741 tcaacaagga cccgaacgcc ccgatcttcg agttctccga tctcggtatc gtcggcgatc
    16801 tcaacaagat cctccccaag ctgaccgagg ccgtgaaggc caagagggga ggctgatccg
    16861 acgatgggcg cgcgcccggc ggatttcccg ccgcccgtca agtcctacga ggagttcgtc
    16921 ggccagccga cggatcccga ggacgagcgg atcgaggtag gcatcgtgat cgtcggaggc
    16981 ggccctgcgg ggctcgcctg cgcgatccgg ctgatgcagc tgctcgaaga cgcaccgcag
    17041 ctcgccgagt cgctcggcga ggtgccggtc gcggtcatcg agaagggcaa gaacaccggc
    17101 tcgcacctgc tgtcgggggc gatgatgaat ccgtcggcga tgcgcgagct gttcccggac
    17161 gtgcccgagg aggactggcc tacgtacggc cccgtctcca aggacgcggt ctacctgatg
    17221 accgacaaga cggccacgcg cctgccgacg ccgccgccgt tccgaaacca cggcaaccac
    17281 gtcacctcgg tggctcagct cgggcgctgg ctcggcgaga aggccgagga ggccggtgtg
    17341 tacgtgctta ccgagtcggc gggctacaag ctgctcgtcg acgacggcca ggtggtgggc
    17401 gtgcgcaccg gcgacaaggg tcgcgggcgt gacggcgagc cgctcccgaa cttcgagccg
    17461 ggctcggacg tgatcgcgaa ggcgaccgtg ctggccgagg gcacgcaggg ccacctgaca
    17521 ggcgcggcaa tcggtcgctt cggcctgggc gccgacgacc cgcagcagtg ggagatcggc
    17581 gtgaaggagg tctgggaggt caccaagccg ctcgaccgcg tgattcacac gatgggctgg
    17641 ccgctcaagc tgagctcccg gtatggggag ttcggcggct cgttctgcta cccgatgggc
    17701 gacgacaagg tgtcgatcgg cttcgtcgtg gggttggact acaaggacgc gaacttctcc
    17761 gtccacgaca cgctccagct gatcaagacg caccctctgt tcaagaaggt gctcgagggc
    17821 ggcaagcggg tcggatgggg ggcgaagacg ctcccgtccg gcggctactg ggcgatgccc
    17881 agccagctct gggccccggg gctcgtcctc gcgggcgacg gcgccggcat ggtgaatgtg
    17941 ccgaagctga agggtgtcca ctacgcgatg cacgccggca tctacgccgc cgaggcgatc
    18001 ttcgacgggc tcaagaaggg cgacacgagc gacctctcgg gctacgagga ccgcgtgcgc
    18061 gacagcgtga tcgagaagga cctctaccgc tcgcgcaaca tgcgccagcc gttcacgcgc
    18121 gggttcttcg tgggcggtgc gatcgcgaat gtgctcgagg ccagcggcgg gcgcctgccc
    18181 cccggccact tcgccactca cgatgacgcc gaggtggagg tgttccaggg caagaagcgg
    18241 gactacccga agcccgacgg gaagctcacg ttcgacaagc tctcgagtgt gttcgccacg
    18301 ggcaacgcga cccgcgacga cgcgcccaac cacgtccgtg tccagcgcaa cgtgccgatc
    18361 gagatcgcgc tgatgtggaa gaacatgtgc ccggcgtacg tgtacgagct ggcggacgag
    18421 gtgcaggagc agctcgacaa tggcggcatc cccgacggga cgctcgtcga ggttcatctc
    18481 acgccgtcga actgcgtcca gtgcggcgcg atcacggcga agggtggccg cctcaccccg
    18541 ccggagggcg gagacgggcc caactatcag ctgacctgag tctcagagcg gcgtcctcgg
    18601 gcgccgctct tcgttcggca tagactcggc acgtggatct cgcggtcggg atcgccgcgt
    18661 cggtcgtggc ggctctcatc ttcaccgggt cggtgaccgt ccaggcgctc gaggcccggg
    18721 cggtgtcgca tgagcacgcg atgcgcgcca cgctgctgag gagcctcgcg gcgcgccgcc
    18781 gctggctcgg cgcgatcagc ctgctcgtgc tcggctggat cttctacgtg ttcgccctta
    18841 cgctcgcgcc gctgacggtg gtgcagccgg tccgcgccgt cgggctgctg gtgcttctgt
    18901 gggtgggcgt gcgggtgctg ggcgagcgcg ccgggcggcg cgagctgatc ggcgtgctgc
    18961 tgatcgtggg cggcgtcgtt gcgctcggcg cggctgctcc cgagcacagc agcaaccacg
    19021 cgagtgcggg ggagctggtc ctggcgctcg ggctgctcgg cgccgcggcc ttggctccct
    19081 tcgccgtacg cggcggcgat cgcggcctga gcctcctcgt ggtgttcgcc gcggggctcg
    19141 cgttctcgtg ggtggacttc gcttcgaagc tcatggccga cgcgctgcac gatcaccact
    19201 tcctcggagc gtgggcctgg gcgacggcga tcggtgtgtt cgcggtcgtc ggtctactcg
    19261 ccgagaactc gtcgttccag cgccggccgg ccactcaggt ggtgccgatc gtgttcgcgc
    19321 tgcagatggc actgccggcc gcgctggccg cgctcgtggg cggagagaac tggggcgaca
    19381 cgccgctcgg cggggcggtg atcggcgttg cgcttgtgct cgtgaccgtg gggacgctgg
    19441 tgctggcgag ctcgcacgcg gtggcggacg ttgtggccgc tgcggagggc gccgaggagg
    19501 cggaacccgc tgccgcgtag cagctctggt tagcggtaag gggagggagg cgtacgcggt
    19561 ctggccgcgc ggtttcctcg caccgtggtc gattttgccg ctgtgggcgg cacgatcgac
    19621 caatgtgcac tgaattcgcc gaagcgtcct gctttgccca cgcatagcgg ggggtaatcc
    19681 aggacggtag agagaaaaca gccgctctca cccccttatt gctaacccgg agaacccgcc
    19741 cgccggcgga cacgggtacg gtgccgaagc aggatgccgc gcacacgcca gcccatacgg
    19801 cgcgccagcg tggtcggagc gggttcgttc gggacggcgg tcgcggcgct gctcgtgcgg
    19861 gcggggatcc gcaccacact cctctgccgc actcaggagc aggccgacga gctctccgcc
    19921 agggctgaga acgagcgcta cctgaagggc gtgaagctgc ccgaccgcct caaggtgcgg
    19981 gtgctcggag gacgggagga ccagttcggc cgggcggacc tggtgctgct ggcggtgccc
    20041 tcggccggcc ttcgcgccgc aatcgacgag ctactgcggc agggtgtccc tcgcacgacg
    20101 gcggtggtct ccctcgcgaa gggcctcgtg c
//
LOCUS       DASVXS010000162        22988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_397967, whole genome shotgun sequence.
ACCESSION   DASVXS010000162 DASVXS010000000
VERSION     DASVXS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22988
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397967"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6340)
                     /locus_tag="VF752_09080"
     CDS             complement(<1..6340)
                     /locus_tag="VF752_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8855744.1"
                     /translation="MSGQFTSEPRLEFDFAVLSPAGSPEPSLAIAASRAGALGVLNLE
                     FATDHGAALNELARLSQLGQGRCAALIADEELLTVALAERFDALETIVLPDARVERLD
                     ELVRRIHGAGLQAHVIATRLDDALAAVDAGADAVIAKGHEAGGWVDEEGSFVLLQRLI
                     AAVPTPVWAYGGIGLYTVNAAYVAGAAGVVLDAQLLLARESPLPDSVRSRIASMDGSE
                     TEVLGSRIGAPFRVYSRPGAEGCDALRESEARLETTADALDTWRTAVSERVGWDSSDA
                     VLPIGQDASFANDLAERFQTVGGILGAFKESVVDGWDILQRSNPLATGAPLAESHGTT
                     YPIVQGPMTRVSDRADFAAAVAEAGALPLLALALMRAPEVHELLERTGQLLGDRPWGV
                     GILGFVPPELRAEQLDVLLEYRPPFALIAGGRPDQARALEDEGIATYLHVPSPGLLKL
                     YLADGARRFVFEGRECGGHVGPRTSFVLWDTMVRTLLDELPDGSDDCHVLFAGGIHDA
                     TSAAMLAGTASALSDRGVRVGALLGTAYLFTHEATEHGAITERFQEAAIAGRDTALVE
                     SGPGHATRCLPSPFVDQFNAEKRRLRDEGVAADELRGRLEQLNIGRLRIASKGADRNP
                     RFGDDPEAPKLVEVDPSDQWERGMYMIGQVAALRDRTTSLADLHRDVSAGSSELIDAL
                     PAPEVARQAPPAQADVAIIGVGCILPGAGDASTFWANIMDKVDAIREVPEDRWDWRRY
                     YDADPSARDKIYSRWGGFVDEVRFDPIAFGLPPNSLTSIEPFQLLALLTASAALSDAG
                     YAMRPFDRERTSVVLGAGGGGADLAVGYTVRSALPSLLGDAKPDLGERLNERLPEWTE
                     DSFAGLLMNVAAGRIANRLDFGGANFTVDAACASSLAAIGLGARELQSGTSDMVLAGG
                     VDAIQNPFAYLCFSKTHALSPNGHCRPFDAAADGIAISEGFATVVLKRLADAERDGDR
                     IYAVVRGVGAASDGRDRSLTAPRPEGQMRALRRAYAQARFSPASVELVEAHGTGTVAG
                     DGAEVQALSRLFAEHSEDTQWCAIGSIKSMIGHTKATAGVAGLIKAALALHHRVLPPT
                     IGVSEPNPKANFPESPFYVNTEARPWLSGGDSHPRRAGVSAFGFGGTDFHIVLEEYTG
                     DYLRRDESAVDRWPAELFLWRGTRDDISASLGSLAARLTVDHDYRLADVARTVALEAT
                     TPEPGHGTLAVVADSLDDLQAKLSQARELLDGSDARVHTPNGIHWSEQPLAADARVAF
                     LFPGQGSQFVGMGREIALAFPEARECFERADRTLADRYDRPLSRYIFPPPAFKPEDEQ
                     RQQAELTDTHVAQAALGATDLAYLHVLEALGIEPEMVGGHSYGEFVALAAANGLGVDD
                     LLRLSEARGRFMGEAAADAPGAMAAVDATPEMLEPLLSEGEVVAANLNAPDQTVLSGL
                     QEHIDAAIEWCRERDIAARALPVACAFHSRYVAPAQQRLAAVLDTTRLDSPGIPVFSN
                     TTGERHAEDPAEIATLLREHLGSRVDFVSEIDAMYRDGARVFIEVGPRSVLTGLVGRV
                     LGEREHLAVPVDRPGRPGLTQLLHCLAAVAAEGVPLRMERLFRGRSAERIELDATDPS
                     ARAHPPGTWLVHGGRARPADVPFEPPAPITSHREEELPVTNTSDGGAPPGQALAPVAT
                     NGQAGELAPAEPISGEPIAGDRVGDVMGRYHQVMQHFLETQRSVMLAYLGAPRPAVDV
                     RPSPARSTPMPRPPATPVQPPQPAAPPVVEAPAVPDAALAVPDAAPEAVAPPPTGNGH
                     GALSAEEIKDRLLSVVSERTGYPAEMLGLDADLEGDLGIDSIKRVEIAGTLAQTLTLP
                     GGGELDVERLTASRTLREVIAALEEAVAPNAAPAEDLSPEDLSPFEDGPAEEERIGRF
                     VLQAASAPAITATAGLAAAGAVVIVDDETGVGEALADALAVQGEASVRLAPADLPEDA
                     EAATQLAERLREEHGGAKALVHLAALGVCGGSDAGLPTLLLLAQALREHLEHAAAGGG
                     AVVLGASRLGGSFAVDGQVPEGAAPEAAVPGFLKTLAQEWPAMRVKAVDLSAAPREED
                     ASHLLAEMAAADGLVEVGYRDGQRTDLRLVPASLADRAD"
     gene            6562..8541
                     /gene="cysC"
                     /locus_tag="VF752_09085"
     CDS             6562..8541
                     /gene="cysC"
                     /locus_tag="VF752_09085"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX8855745.1"
                     /translation="MSVLSPSSARFTPLERLERLCEPGSLQRLGPVSDRVGVEAGRGL
                     VHGRPIVCYAQNSTVAGGSVGVAEADVVVRALRLCREEGIPLVAFLESAGARLQEGAA
                     ALGGFGRIFSENVALSGHAPQISVITGTSAGGGCYSPALTDFVVMTEEASMFLTGPKI
                     VKQALGEEVSASELGGPQVHERNGVCHFVAADDRQAIHLTRELLGYLPQNASEPVHPI
                     PAEAPSGADPCSALPARARSFYDIRTVLRCLVDSGQFLEVSPRWARNMVVGFARLEGH
                     SIGIIANQSRHLGGVIDVAASQKGAKFVRTCDEFGLPLLVLVDTPGFMPGTRQEGAGI
                     IRHGADLLRAFAATTSPRVTVIVRKAFGGAFITMNSKDLGATASFAWPTAEIGIMSPR
                     GAVEIIHRRRLDSAASAEDEADSLASHYARAHLSPDVALRLSAIDDVVEPAETRERVA
                     GALFSPRQGRRARRPLRASARQGCTVWFTGLSGAGKSTLAALVERELRARGERVEVLD
                     GDVVRRNLSQGLGFSKEDRDTNIRRIAFVADALSRNGVFVLAAAISPYRATREEARQL
                     MGDRFVEVYVEASVDECARRDTKGLYAKAFAGEITEFTGVSDPYEPPPAPEIVVDTET
                     EAPEESAAKVLAFLDSRLHRNGRPGRQGAPWPSSA"
     gene            8523..9608
                     /gene="sat"
                     /locus_tag="VF752_09090"
     CDS             8523..9608
                     /gene="sat"
                     /locus_tag="VF752_09090"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX8855746.1"
                     /translation="MAELRLSARQTADFELLANGGFAPLDGFQGRADWESVLERMRLA
                     DGRPWPVPITLGSSQGRIGETLVLRAPDETVLGALTVEEVYERDLLHEAEAVYRTTDE
                     SHPGVAALLLESARTVAGPIEVRALPRHPEAVSRHLLTPMEARREFNERGWRTVVGFQ
                     TRNPIHRAHEYLTKAALEIVDGLFIHPIVGETKSDDIPVETRLRCYEVLIERYYAADR
                     VVLGANPAAMRYAGPREAILHALVRRNYGCTHFIVGRDHAGVGSYYGSYDAQRIFDEF
                     DDGELGIEPLFFEHAFFCRVTGEMATPKTSPGGPEDRVFLSGTKVRELLLRGERPPEE
                     FTRPEVADILIEAMNARAPEPAGMHRE"
     gene            9635..10621
                     /locus_tag="VF752_09095"
     CDS             9635..10621
                     /locus_tag="VF752_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8855747.1"
                     /translation="MLLAAAITALLAAAGCGSSSSSGGGGNKLSLVAYSTPQEAYEAI
                     IPAFQKTSAGRGVKFTQSYGASGDQSRAVAAGLPADVVALSLEPDVTKLVEGGLVSPD
                     WNKDKWHGFVTNSAAVFAVRKGNPKNIRTWDDLVKPGVQVVTPNPFSSGGAKWNIMAA
                     YGAQLEQGKSPAQAQAFLRALLKNTVVQDKSARDALQTFTSGKGDVLISYENEAITAQ
                     QKDQALDYVIPDQTILIQNPVAAVKNGNVDKAKAFIAFLHSANAQKIYASHGYRSTDP
                     ALVDKRKYPTPPGLFTIDKFGGWGAVNDKFFDPQNGIVTKIEQGLGVSTAKG"
     gene            10695..11438
                     /gene="cysT"
                     /locus_tag="VF752_09100"
     CDS             10695..11438
                     /gene="cysT"
                     /locus_tag="VF752_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX8855748.1"
                     /translation="MILWLSVIVLLPLAAVVVKSTDGGLAGFWDAVTSPQSVAAIKFT
                     LLTSFVVVVINAVMGTVIAWVLVRDDFAGRRFVSALIDLPFALPTIVAGLTLLALYGP
                     RSPIGVNVAFTKAAVVLALLFVTLPFVVRAVQPVLMELDREMEEAAASLGARPLTIFR
                     QIILPNLQPAIVAGCSLGFARALGEFGSVVLISGNIPFKTEVASVFIFSQIGNDNVTG
                     AAAVSVVLLALSMVVLALVARLGRSWYDG"
     gene            11428..12219
                     /gene="cysW"
                     /locus_tag="VF752_09105"
     CDS             11428..12219
                     /gene="cysW"
                     /locus_tag="VF752_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX8855749.1"
                     /translation="MTARLGLRATALGYLTLLLVVPVGMVFWRAFEHGFGAAWNAVTT
                     PDALHALQLTLLMVAVAVPLNTVFGIAAALLLVRRRFRGKPILEAIIDLPFAVSPVVV
                     GLALVLVYGRVGWFGNWLSEHGIQVIFSTWGMILATIFITLPFVVREVAPVLREIGTE
                     QEEAARTLGASPWQTFSRITLPAIRWGVGYGVVLTTARAIGEFGAVSVVSGHLVGQTE
                     TLTLFVQDRFERFDLAGAYAASVVLAAIALSTVAVMTLLQKRRGA"
     gene            12219..13208
                     /locus_tag="VF752_09110"
     CDS             12219..13208
                     /locus_tag="VF752_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEX8855750.1"
                     /translation="MSIVVVNVSKRFGAFTALDGVALEVPDGSLTALLGPSGGGKSTL
                     LRVIAGLEAPDEGRVLIGGSDVNAVPARERGVGFVFQHYAAFKHMTVRANVAFGLKIR
                     KREKAYIQRRVDELLELVHLEGFADRYPSQLSGGQRQRMALARALAVEPRVLLLDEPF
                     GALDATVRKELREWLRRLHDDIHVTTVFVTHDQQEAMEVAEQIAVLNDGRIEQTGSPR
                     ELYEQPANPFVMRFVGPVSELDGEWLRPHDLDILLARSEDAIEATIERVAYLGFEVRV
                     ELALPNGDSTWVQTTRARAGELDLRTGQNVWLQTARTAGPQAAPPTLTTAPSR"
     gene            complement(13205..13366)
                     /locus_tag="VF752_09115"
     CDS             complement(13205..13366)
                     /locus_tag="VF752_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855751.1"
                     /translation="MNVSALLKSKSKRIQGQKIVKKPITHGNHTIGEEQTTLRWTLHL
                     QRVGKVSRF"
     gene            complement(13820..14233)
                     /locus_tag="VF752_09120"
     CDS             complement(13820..14233)
                     /locus_tag="VF752_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855752.1"
                     /translation="MRLNSRRARVYLATGLVGFIVIGTVVGALVSQSGGGDTCRNFRF
                     DPVKWQQAKLNVNPPTDRQELADDLLRCHRLDDLSKTEVLQMLGPGVPRLSNNSRTMW
                     VTGLVRGSLSTGREYLRVTYDANGTVVKAALANGE"
     gene            complement(14261..15091)
                     /locus_tag="VF752_09125"
     CDS             complement(14261..15091)
                     /locus_tag="VF752_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8855753.1"
                     /translation="MPAGAVTADGRARVLADQNEMPLLGLGVWQVPNGPECVNAVRWA
                     LELGYRHLDTAQAYGNEESVGEGIRQSGVPREDIFITTKFYPARQDPEAEAERSLRRL
                     GVDQVDLYIIHWPQGGPTWAWPGMERALAAGYTRSIGVSNFSAGELDEVMGIAEVQPV
                     VNQAQFSPFEFRRKLLEACEEKGIALEAYSPLGTGRHLGDTTVAEIAERLDRTPAQVL
                     IRWSLERDLPVIPKSTHRERLEENAQVFDFSLSDEDMAALDALDETGGTDRALERRWW
                     "
     gene            complement(15192..15626)
                     /locus_tag="VF752_09130"
     CDS             complement(15192..15626)
                     /locus_tag="VF752_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8855754.1"
                     /translation="MTREEIIRQFDAQDAAFNRHDVGAVAATYAEDARMRDQAAPGPI
                     EGREAVGEYIGGYLRAFPDLQWERISVEVDGNVGIEEWRASGTHEGDLPGLPATHRQM
                     TIDGCSVMQFGDDGLIREEHNYWDEAAMLRQLGVMAEPAPAR"
     gene            complement(15710..16369)
                     /locus_tag="VF752_09135"
     CDS             complement(15710..16369)
                     /locus_tag="VF752_09135"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8855755.1"
                     /translation="MALQIGDTAPDFEAETSEGPIRFHDWIGDSWAVLFSHPKDFTPV
                     CTTELGYMAKIKPDFDSRNVKIIGLSVDPVDKHQQWADDIEDTQGFRPNYPVIADSDF
                     NVSKLYGMLPSETSGDPADRTPADNQTVRNVFVVGPDKKVKLILVYPMTTGRNFDEVL
                     RVIDSLQLTAKHKVATPVNWQRGDDVIIAGSVSDDEARDTYPEGWESPRPYIRIVPQP
                     K"
     gene            complement(16477..17370)
                     /locus_tag="VF752_09140"
     CDS             complement(16477..17370)
                     /locus_tag="VF752_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855756.1"
                     /translation="MSAQTAIALALALASTTLTNIAYLREHDAASALPPLTLRHPVHG
                     ARLLLTSRSWLVGFAMETTGFLLYAAALALAELALVQSILAGGIGVLAFVSSRMSGRP
                     LGQRELRGVLLSLLGLLALAVSLAGGSEEGSGGSLAGISLWLGACAAAAVLALTVGRH
                     VMGIAVANGVAGGLFFAIGDVSTKVATDGGLHSAFILTLIAGYTLGTALLQVGYQAGG
                     ALTVAGLATLLTNAVPIAAGTIVLHEPVPSGALGALRILAFAAVSTGAYLLARPEQPS
                     TGRPGHAQGASQTAEAKGSGS"
     gene            17436..17732
                     /locus_tag="VF752_09145"
     CDS             17436..17732
                     /locus_tag="VF752_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855757.1"
                     /translation="MIVMFSARRLKPGAWEQFRQAWEPGDAKPPGFQRAYHARNIRDE
                     DEVISFGLFDMSKDDYHRWREEADAQETHRVDRMSAFVENELVSGVYEVIDVAE"
     gene            17733..19745
                     /locus_tag="VF752_09150"
     CDS             17733..19745
                     /locus_tag="VF752_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855758.1"
                     /translation="MTAVAAPAQTAVPQRRVLVIFGALMLVLLLAALDQTIVSTALPT
                     IVGDLGGLAHLSWVVSAYLLAQTAVTPIYGKLGDLYGRKRILQIAVVLFLAGSALCGA
                     SQNMSELIAFRAVQGLGGGGLIVLTQALVGDVVSPRERGKFQGLFGAVFGVASVAGPL
                     LGGFFVDNLSWRWIFYINLPLGLVALVVLGIVLPGRGEIARPKIDYLGSGLLAGAVSC
                     VVLATSLGGNTWPWDSAQLIITACAGVVLGVLFVLVERTAPEPVLPLRLFQNRTFTIA
                     GLIGLIVGFALFGSAVFLPLFFQTVNGVDPTTSGLRLLPLIAGLLVTSIGSGQVISRI
                     GRYKPFPIAGTACMVIAFYFLSKMDAGTSALEAAWRLVILGLGLGFVMQILIVAVQNA
                     VSYRDLGVATSGSTLFRLIGGSIGTAVFGAIFSNRLSSELAGRVPAAAQQQGRLSPAQ
                     LRHLPPQAHDAYIQAFTNALSDVFLVAAAVAFLGFLLALALPDQRLRDTVEAAGPQEH
                     FAVPRGDDTLGEIERALSVLARRDVRKRLYERLARDAGIDLPALEAWTLARISEGAPG
                     PAKGLADRIGVESARVSQATVELERRGLVVRGDGWFASTRTGDDLVEQLIRLRRERLA
                     ARLADCTPEERQQFAQVLDRLARDLLAEPPGDRQPASAGPVSSSDR"
     gene            complement(19726..19986)
                     /locus_tag="VF752_09155"
     CDS             complement(19726..19986)
                     /locus_tag="VF752_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855759.1"
                     /translation="MLRCRVFGHRFRFTSSGNEMRWTCQRGCGASASKRYATAADARR
                     YAAAFDREDRADLGRRAPLFGLLPLRIARAIRDRGKVSGRSS"
     gene            complement(20085..20579)
                     /locus_tag="VF752_09160"
     CDS             complement(20085..20579)
                     /locus_tag="VF752_09160"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX8855760.1"
                     /translation="MSSTSQDRITEPWSARAAPPLAERARRLARGFWFAELLLVAGLA
                     WPTYHVRDEMFTNFGDHPWRLIFPALALVALAAQFILQGMERWGRAFFASSLFTTMAA
                     GLYPNVLPAHDNRPFGMTIHNAASSEHALTVGLRWWSLGIALAVGYFIVAYRFLFRRA
                     TRTP"
     gene            20569..20832
                     /locus_tag="VF752_09165"
     CDS             20569..20832
                     /locus_tag="VF752_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855761.1"
                     /translation="MELMHDAFENGPDFSELPTYVEDTGEVKWVLEWALRRDIPSPVI
                     SESQEALMAYRDLEWPAAKAVALLRHEYGGHPLHRAEEQFVRT"
     gene            20837..21892
                     /locus_tag="VF752_09170"
     CDS             20837..21892
                     /locus_tag="VF752_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX8855762.1"
                     /translation="MVSRAGAASRTAELRSPSGLLEATFVPRTGMLGWSLRDGDDELL
                     GHPVSLERYAETGEPTGIPLLHPWANRLASPEYEIAGRRVVLDMNAPNVLKDPNGLPL
                     HGLAAASPHWEVLDEEPPRLRARLDYAAWPELERGFPFPHLLELTVTLADDRLETATE
                     LIPTGESPVPVAFGFHPFLRIPGLDRRQWHVELPVTSRMVLDERELPTGRSEPVHIPP
                     GPLGDRTFDDCFDGLGSGEFAVSGAGRRLHVRFLEGYRFAQLFAPPGSDFICFEPMTA
                     PVNPFESEHTLLAEPVSRYRARFEVGVTKVWLGPASVPTRVRSAVSVISRPPRPRVKK
                     LYDQRTRTTSRFSKPIR"
     gene            complement(22129..22950)
                     /locus_tag="VF752_09175"
                     /pseudo
     CDS             complement(22129..22950)
                     /locus_tag="VF752_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         3321 a         8017 c         8045 g         3605 t
ORIGIN      
        1 cgtcggcgcg gtcggcgagc gaggcgggga ccagcctcag gtcggtgcgc tgcccgtctc
       61 ggtagcccac ctccacgagg ccatcggctg ctgccatctc ggcaaggagg tgcgaggcgt
      121 cctcctcccg cggcgcggcc gacaggtcca ccgccttcac tcgcatcgcc ggccactcct
      181 gcgccagcgt ctttagaaat cccggaactg ctgcctcggg cgcggcgccc tccggcacct
      241 gcccatcgac ggcgaagctt ccgccgaggc ggctcgcccc gagcaccaca gcgccgccgc
      301 cggctgctgc gtgctcgagg tgctcgcgca acgcctgcgc taggaggagc agggtcggaa
      361 gcccggcgtc gcttcccccg catacgccga gcgccgccag gtggaccagt gccttggcgc
      421 cgccatgctc ctctcgcagc cgctcggcga gctgcgtcgc cgcctcggcg tcctcgggca
      481 gatcggccgg cgcgagccgg accgaggcct cgccctgcac ggcgagcgcg tcggccagcg
      541 cttcgcccac gccggtctcg tcgtcgacga tcaccacggc gccggcggcg gccagccccg
      601 ccgtggccgt gatcgcggga gcgctcgcag cctgcagcac gaaccggccg atgcgctcct
      661 cctcggccgg tccatcctca aaaggggaga ggtcctcggg ggagaggtcc tcggctggtg
      721 ctgcgtttgg cgcgaccgct tcctcgagcg cggcaatcac ctcgcgcagc gtgcggctgg
      781 ctgtgagccg ctcgacgtcg agctcgccgc cgcccggcag cgtcagcgtc tgtgcgagcg
      841 tcccggcgat ctcgacccgc ttgatcgaat cgatgccgag gtcgccctcg agatccgcgt
      901 cgagccccag catctccgcc gggtagcccg tccgctcgct cacgaccgag aggagccggt
      961 ccttgatctc ctccgcgctg agggctccgt gcccgttccc cgtcggtggc ggagcgaccg
     1021 cctcgggcgc ggcgtccggc acggcgagcg ccgcatcggg cacggccggc gcctcgacga
     1081 ccggaggcgc cgccggttga ggcggttgca ccggcgtagc gggcggccgg ggcatcggcg
     1141 tagaccgagc tggactcggc cgcacgtcca cggcgggccg cggcgcgccg agatacgcga
     1201 gcatcaccga gcgctgggtc tcgaggaagt gctgcatcac ctggtggtag cggcccatca
     1261 cgtccccgac ccggtcgccg gcgataggct cgccgctgat cggctcggcc ggcgcgagtt
     1321 cgcccgcctg gccgtttgtg gcgacgggcg cgagcgcttg gcccggcggc gcgccgccat
     1381 cgctggtgtt cgtcacggga agctcctctt ctcgatggct ggtgatcggg gctggtggtt
     1441 cgaaaggcac gtcggcgggc ctcgcgcggc cgccgtgcac gagccaggtg ccgggtggat
     1501 gagcccgtgc actcggatcg gtcgcgtcga gctcgatccg ctctgccgag cgaccgcgga
     1561 acagccgctc catgcgcagc ggcactccct cggctgcgac cgcagcgagg cagtgcaaga
     1621 gctgcgtcag cccgggccgg cccggccggt cgaccggcac ggcgagatgc tcgcgctcac
     1681 cgaggacccg gccgactagc ccggtcaaca ccgagcgcgg cccgacctcg atgaacacgc
     1741 gtgcgccatc gcggtacatc gcgtcgatct cgctcacgaa gtcgacgcgg ctgccgaggt
     1801 gctcgcgaag cagcgttgcg atctccgcgg ggtcctcggc gtggcgctcg ccggtcgtgt
     1861 tcgagaacac agggatcccc ggcgagtcga gccgtgtggt gtcgagcacc gcggccaagc
     1921 gctgctgagc gggcgcgacg tagcgcgagt ggaacgcgca tgccacgggc agagctcgcg
     1981 cggcgatgtc gcgctcgcgg caccactcga tcgccgcgtc gatgtgctct tgcagccccg
     2041 acagcaccgt ctgatccggc gcgttgaggt tggccgccac cacctcgccc tccgacagga
     2101 gcggctcgag catctccggc gtggcgtcga ccgcggccat tgcgcccggc gcgtccgccg
     2161 ccgcctcgcc catgaagcgg ccgcgcgcct cggagagccg cagcaggtcg tcaacgccga
     2221 gcccgttcgc cgcggccagg gcgacgaact cgccgtagct gtggccgcca accatctcag
     2281 gctcgatgcc gagcgcctcg agcacgtgca ggtaggcgag gtccgtggcg ccgagcgctg
     2341 cctgggccac gtgggtatcc gtcagctccg cctgctggcg ctgctcgtcc tcgggcttga
     2401 acgccggcgg cgggaagatg tagcggctga gtggcctgtc gtagcggtcg gcgagcgtgc
     2461 ggtccgcgcg ctcgaagcac tcgcgcgctt cggggaacgc cagagcgatc tcgcggccca
     2521 tgcccacgaa ctgcgaaccc tggcccggga acaggaacgc gacgcgagca tcagccgcca
     2581 acggctgctc ggaccagtgg attccgttcg gcgtgtgcac gcgggcgtcg ctgccgtcga
     2641 gcagctctcg cgcctgcgag agcttcgcct gaaggtcgtc gagcgagtcg gccacgacgg
     2701 cgagcgtccc gtgtcccggc tccggagtgg tcgcctcgag cgccaccgtg cgggccacgt
     2761 cggcgagcct gtagtcgtgg tcgacggtga gccgggcggc gagtgagccg agcgacgcgg
     2821 agatgtcgtc gcgcgtgccc ctccacagga acagctccgc gggccagcgg tcgacggcgc
     2881 tctcgtcccg gcgcaggtag tcgcccgtgt actcctcgag aacgatgtgg aagtcggtgc
     2941 cgccgaagcc gaatgcgctc acgcccgcgc ggcgcggatg cgagtcgcca cccgacagcc
     3001 acggccgggc ctcggtgttg acgtagaacg ggctctcggg gaagttcgcc ttcgggttcg
     3061 gctcgctcac gccgatcgtc ggcggcagca cgcggtggtg cagcgccagc gccgccttga
     3121 tgagccccgc tacgcctgcg gtcgccttgg tgtgaccgat catcgacttg atcgagccga
     3181 tcgcgcacca ctgggtgtcc tcgctgtgct cggcgaacag ccgggagagc gcctgcacct
     3241 cggcgccgtc gcccgccacc gtcccggttc cgtgtgcctc gacgagctcc acgctcgcgg
     3301 gggagaaccg cgcctgcgcg tacgccctgc gcagcgcccg catctgaccc tccggccggg
     3361 gcgccgtgag gctgcggtcg cgcccgtcgc tcgccgcgcc gacgcctcgc acgacggcgt
     3421 aaatgcggtc gccatcgcgc tcggcgtcgg ccagccgctt cagcacgact gtcgcgaagc
     3481 cctcgctgat ggcgatgcca tcagcagccg cgtcgaacgg ccggcagtga ccgttcggtg
     3541 agagggcatg cgtcttgctg aagcagagat aggcgaacgg gttctggatc gcgtcgacgc
     3601 cgcccgcgag caccatgtcg ctcgttcccg actgcagctc gcgcgcgccg aggccgatcg
     3661 ccgcgagcga ggaagcgcac gctgcatcga ccgtgaagtt cgcgccgccg aagtcgagcc
     3721 ggtttgcgat ccgcccggcc gcgacgttca tcaggaggcc ggcgaagctg tcctcggtcc
     3781 actccggcag gcgctcgttc aggcgctctc cgagatcggg cttggcgtcg ccgagcagcg
     3841 acggcagtgc ggagcgcacc gtgtagccga ccgccaggtc ggcgccgccg ccgccggcgc
     3901 caagcacgac cgaggtgcgc tcgcggtcga acgggcgcat ggcgtacccg gcgtcgctga
     3961 gcgcggcgct cgcggtcagc agcgccaaga gctggaacgg ctcgatcgag gtgagggagt
     4021 tcggcggcaa cccgaacgcg atcgggtcga agcgcacctc gtccacgaag ccgccccagc
     4081 gcgagtagat cttgtcgcgg gcggagggat cggcgtcgta atagcgacgc cagtcccagc
     4141 gatcctccgg cacctcgcgg atcgcgtcga ccttgtccat gatgtttgcc cagaaggtcg
     4201 aggcgtcgcc ggcgccgggc aggatgcagc cgactccgat gatcgccacg tcggcctgag
     4261 ccgggggcgc ctgtcgcgcg acctcgggag cgggaagagc gtcgatcaac tcggagctcc
     4321 cggccgagac atcgcggtgg aggtcggcca gcgaggtggt gcggtctcgc agggccgcga
     4381 cctggccgat catgtacatc ccccgctccc actggtcgga cgggtcgacc tcgacgagct
     4441 tcggtgcctc cgggtcgtcg ccgaaacgcg ggttgcggtc ggcgcccttg ctggcgattc
     4501 ggagccggcc gatgttgagc tgctcgaggc gaccgcgcag ctcgtcggcg gccacgccct
     4561 cgtcgcggag ccggcgcttc tcggcgttga actggtccac gaacggcgac gggaggcagc
     4621 gcgtggcgtg acccgggcca ctctccacga gagcagtgtc gcggcccgcg atcgccgctt
     4681 cctggaaccg ctcggtgatc gccccgtgct cggttgcctc gtgcgtgaac aggtaggccg
     4741 tgccgagcag ggcgcccact cgcaccccgc ggtcggacag cgccgacgcg gtgcccgcca
     4801 gcatcgcggc ggacgtcgcg tcgtggattc cgcccgcgaa caggacgtgg cagtcgtcgc
     4861 taccgtcagg aagctcgtcg agcagcgtgc gaaccatcgt gtcccagagc acgaagctgg
     4921 tgcgtgggcc cacatgcccg ccgcactcgc ggccctcgaa gacgaagcgg cgcgcgccgt
     4981 cggcgaggta cagcttcagc agccccggag agggaacgtg gaggtaggtc gcgattccct
     5041 cgtcctcgag tgcccgagcc tgatcgggcc ggccgcccgc gatcagggcg aacggaggcc
     5101 gatactcgag cagcacgtcg agctgctccg cgcgcagctc cggtggaacg aagccaagga
     5161 tgccgacccc ccacggccgg tcgccgagca gctggccggt gcgctcgagc agctcgtgta
     5221 cttcgggcgc gcgcatgagc gcgagcgcga gaagcggcag cgcgcccgcc tctgcgaccg
     5281 cggcggcgaa gtccgcgcgg tcgctcactc gcgtcatcgg gccttggacg atcggatagg
     5341 tcgtcccgtg cgactcggcc agcggcgcgc ccgtcgcgag cgggttgctc cgctgcagga
     5401 tgtcccagcc gtccacgacc gattccttga aagcgccgag aatcccgccg acggtctgga
     5461 accgctcggc gaggtcgttc gcgaaggagg cgtcctggcc gatcggcagc acggcgtcgc
     5521 tcgagtccca gccaacgcgc tcgctgacgg ccgtccgcca ggtgtcgaga gcgtcggccg
     5581 tggtctctag ccgcgcctcg ctctcgcgca gggcgtcgca gccctcggcg ccggggcggc
     5641 tgtagacccg gaacggcgcg ccgatccttg agcccagcac ttcggtctcg ctcccgtcca
     5701 tcgacgcgat ccgagagcgc acgctgtcgg gcaggggcga ctcgcgagcg agcaggagct
     5761 gagcgtcgag cacgacgccc gcggcgccgg ccacgtacgc cgcgttcacg gtgtacagcc
     5821 cgatcccgcc gtacgcccac accggcgtgg ggaccgccgc gatcaggcgc tgcagcaaca
     5881 cgaacgagcc ttcctcgtcg acccagcctc cggcctcgtg acccttcgcg atcacggcgt
     5941 cggcgccggc gtccacggcc gcgagcgcat cgtcgaggcg cgtcgcgatc acgtgcgcct
     6001 gcagtccggc tccgtggatg cgccggacca gctcgtccaa ccgttcgacg cgcgcgtccg
     6061 gcagcacgat cgtctcgagc gcgtcgaacc gctccgcgag cgcgacggtg agcagctcct
     6121 cgtcggcgat cagcgcggcg caccgccctt ggccgagctg ggacaagcgg gcgagctcgt
     6181 tcagcgcagc gccatgatcc gtggcgaact cgaggttcag gaccccgagt gcgccggctc
     6241 ggcttgccgc tatcgcaagc gacggctcgg gtgagccggc gggggagaga acggcgaaat
     6301 cgaactcgag ccgcggctcg gacgtgaact ggccggacat tgcgtgtggg gatgaacgcc
     6361 tcgccggaag cgcctcgctc ttccgatggt cgctccagtc gagcccacgc gacccgcctc
     6421 agtcaaggaa tgcgctgcac ctttgcccaa acgggcaact tgcgttgacc gtgctttgag
     6481 gcgctgcata cgctggcccg cgtgcctacc tcgaagtgcg tgctgggcac gatgccagcg
     6541 cggcgattgt gaggtgacgc gttgtcggtc ctctcgccat ccagcgctcg gttcacgccc
     6601 ctcgagcggc tcgagcggct ctgcgagccc ggaagcttgc agcgcctcgg accggtgtcc
     6661 gatcgtgtcg gcgtcgaggc cgggcgtgga ctcgtccacg gacgcccgat tgtctgttac
     6721 gcccagaact cgaccgtcgc cggcggctcg gtcggcgttg ccgaggcgga cgtggtcgtg
     6781 cgcgcgctgc ggctctgtcg cgaggagggc atcccgctcg tcgcgtttct cgaatcggcc
     6841 ggcgcgcggc ttcaggaagg cgccgccgcg ctaggcggat tcggccggat cttctccgag
     6901 aacgtcgcgc tctcgggcca cgctccgcag atttccgtga tcaccggcac ctcagccggc
     6961 ggcggttgct actcgccggc gctcaccgac ttcgtggtga tgactgagga agccagcatg
     7021 ttcctcaccg gcccgaagat cgtgaagcag gcgctcggcg aagaagtgag cgcgtcggag
     7081 ctcggcggcc cgcaggtgca cgagcgcaac ggcgtctgtc atttcgtcgc ggccgacgat
     7141 cgccaggcga tccacctgac ccgggagctg ctcggctacc tgccgcagaa cgcgtccgag
     7201 ccggtgcacc cgatcccggc ggaggccccg tcgggagcgg atccgtgctc ggctttgccc
     7261 gcgcgcgcgc ggagcttcta cgacatccgc acagtgctcc gctgcctcgt cgactcgggt
     7321 cagttcctcg aggtctcgcc gcggtgggcg cgcaacatgg tcgtcggctt tgcgcggctc
     7381 gagggccact cgatcggcat catcgccaac cagtcacggc atctcggcgg cgtgatcgac
     7441 gtggcagcgt cgcagaaggg agcgaagttc gtacgcacct gcgacgagtt cggcttgccg
     7501 ctgctcgtgc tcgtcgacac gcccggcttc atgcccggca ctcggcagga gggggccggc
     7561 atcatcaggc acggcgcgga cctgcttcgt gcgttcgctg ccaccacgtc gccgcgcgtg
     7621 acggtgatcg tgcgcaaggc cttcgggggc gcgttcatca cgatgaactc gaaggatctc
     7681 ggggcgaccg cctcgttcgc gtggcccacg gcggagatcg ggatcatgag ccccaggggc
     7741 gcggtcgaga tcattcaccg gcgcaggctc gactcggccg cttccgcgga ggacgaggcc
     7801 gactcgctgg ccagccacta cgcacgcgca catctgtcgc ccgacgtcgc gctccggctc
     7861 agcgcgatcg atgacgtcgt cgagcccgcc gagacacggg agcgggtcgc gggcgcgctg
     7921 ttcagcccgc gacagggacg tcgcgcaagg aggcctctac gcgcgtctgc gcggcagggc
     7981 tgcacggtct ggttcaccgg cctgtccggc gcgggaaagt caacgctcgc ggcgctcgtc
     8041 gagcgggagc ttcgtgccag aggcgagcgg gtcgaggtgc tcgacggcga tgtcgtgcgc
     8101 cgcaacctct cgcagggcct cggcttctcg aaggaggacc gcgacaccaa catccgccgt
     8161 atcgctttcg tcgccgacgc tctctcccgc aacggcgtat tcgtcctcgc ggccgcgatc
     8221 tccccgtatc gcgccacccg agaggaggcg cggcagttga tgggtgaccg gttcgtcgag
     8281 gtctacgtcg aggcgtcggt cgacgagtgc gcgcgccgcg acacgaaggg gctctacgcg
     8341 aaggcgttcg cgggcgagat cacggagttc accggggtgt cagatcccta cgagccgccc
     8401 cctgcgccgg agatcgtggt cgacaccgag accgaggctc ccgaggagtc cgcggccaag
     8461 gtgctcgcct tcctcgacag ccggctccat cgcaacgggc gccctggccg gcagggagcg
     8521 ccgtggccga gctccgcctg agcgctcgcc agacggcgga cttcgagctg ctcgcgaacg
     8581 gcggcttcgc accgctcgac ggctttcagg gccgcgccga ctgggagtcg gtgctcgaac
     8641 gcatgcgcct cgccgacggg cggccatggc cggtcccgat cacgctcggc tcgtcccagg
     8701 gtcggatagg cgagacgctc gtgttgcgcg cgccggacga aacggtgttg ggcgcgctga
     8761 cggtggagga ggtctacgaa cgcgatctcc tgcacgaggc ggaggcggtc taccgaacca
     8821 cggatgaatc tcatccgggc gtagcggcac tgctcctcga gtctgcacgc accgtcgcgg
     8881 gaccgatcga ggtacgcgca ctgcctcgtc atccggaggc cgtgtcccgc cacctgctca
     8941 cgccgatgga ggcccggcgc gagttcaacg agcgaggttg gcggacagtc gtcggcttcc
     9001 agacgcggaa cccgatccat cgcgcccacg agtacctgac caaggcggcg ctcgagatcg
     9061 tcgacgggct cttcatccat ccgatcgtcg gcgagaccaa gtccgacgac ataccggtcg
     9121 agacgcgcct gcgctgctac gaagtgctca tcgagcgcta ctacgccgct gaccgcgtcg
     9181 tgcttggtgc gaacccggcg gcgatgcgct acgccggacc cagggaggcg atcctgcacg
     9241 ccctggtgcg gcggaactac ggctgcactc acttcatcgt cggccgcgac cacgccggag
     9301 tcggctcgta ttacggctct tacgacgccc agcggatctt cgacgagttc gacgacggcg
     9361 agctcggtat cgagccgctc ttcttcgagc acgccttctt ctgccgtgtg accggcgaga
     9421 tggcgacgcc gaagacgtca cccggcgggc cggaggatcg agtcttcctc tcggggacca
     9481 aggtgcgcga gctcttgctg cggggcgagc ggccgcccga agagttcacg cgccccgagg
     9541 tcgcggacat cctgatcgaa gcgatgaacg cgcgcgctcc cgagcccgcc ggaatgcacc
     9601 gggagtaggc atccgcgcgc gactgatcag cctcgtgttg ctcgcggccg cgatcacggc
     9661 cctgctcgcc gcggccggat gcgggagttc gagcagctcg ggaggcggcg gcaacaagct
     9721 gtccctcgtc gcgtactcga cgccgcagga ggcgtatgag gcgatcatcc cggcgttcca
     9781 gaagacgtcc gcggggcggg gcgtgaagtt cacgcagtcg tatggcgcgt cgggcgatca
     9841 gtcgcgcgcg gtggccgccg gcctgccggc cgacgtggtc gcgctctcgc tcgagcccga
     9901 cgtgacgaag ctcgtggagg gcggactcgt gtcgccggac tggaacaagg acaagtggca
     9961 cggcttcgtg accaactccg ccgcggtgtt cgccgtccgc aagggcaatc ccaagaacat
    10021 caggacttgg gacgacttgg tgaagcccgg cgtgcaggtc gtgacaccga acccgttcag
    10081 ctcgggcggc gcgaagtgga acatcatggc cgcgtacggc gctcagctcg agcagggcaa
    10141 gagcccggcg caggctcaag ccttcctgcg ggcgctgctg aagaacaccg tcgtgcagga
    10201 caagagcgcg cgcgacgcgc tgcagacgtt cacgtccggg aagggcgacg tcctcatctc
    10261 gtatgaaaac gaggcgatca ctgcgcaaca gaaggaccag gcgctcgact acgtgatccc
    10321 ggatcagacg atcctgatcc agaaccccgt ggccgctgtc aagaacggca acgtcgacaa
    10381 ggcgaaggca ttcatcgcct tcctccacag cgcgaacgca cagaagatct acgcgtcgca
    10441 cggctaccgg tcgaccgatc ccgcgctcgt ggacaagagg aagtacccga cgccgccagg
    10501 gctcttcact atcgacaagt tcggcggctg gggtgcggtg aacgacaagt tcttcgatcc
    10561 ccagaacggg atcgtcacca agatcgagca ggggctgggg gtctcaactg ccaagggctg
    10621 aggccatccc ggcggtcgtc aagccgcgag caaggtcccg cctgtcggcg cggccgccgc
    10681 tcgtgggtgg cgtcgtgatc ctctggctca gcgtgatcgt gctgctgccg ctggctgccg
    10741 tggtggtgaa gtcgaccgac ggcggcctcg cggggttctg ggatgcggtt acgagcccgc
    10801 agtcggtcgc cgcgatcaag ttcacgctgc tgacgtcgtt cgtggtggtg gtcatcaacg
    10861 cggttatggg cacggtgatc gcctgggtcc tggtgcgcga cgacttcgcg ggcaggcgct
    10921 ttgtgagcgc gctgatcgac ctcccgtttg cgctgccgac gatcgtggcc ggcctcaccc
    10981 tgctcgcgct ctacggcccg cgctcgccga tcggcgtgaa cgtcgcgttc acgaaggcgg
    11041 ccgtggtcct cgcgcttctg ttcgtgacgt tgccgttcgt ggtccgcgcg gtgcagccgg
    11101 tgctgatgga gctcgatcgc gagatggagg aggccgcggc ctccctgggc gcccgcccgc
    11161 tcacgatctt ccgccagatc atcctgccca acctccagcc ggcgatcgtc gcgggctgct
    11221 cgctcggctt cgctcgcgcc ctcggcgagt tcggctcggt cgtcctgatc agcgggaaca
    11281 tcccgttcaa gaccgaggtg gcgtcggtct tcatcttctc ccagatcggc aacgacaacg
    11341 tgaccggcgc ggccgccgtc tccgtggtgc tgctcgcgct gtcgatggtc gtgctggcgc
    11401 tggtcgcacg gctggggagg tcttggtatg acggctaggc tcggcctccg cgcgaccgct
    11461 ctcggctatc tcacgctgct gctcgtggtg ccggtaggca tggtgttctg gcgggcgttc
    11521 gagcacgggt tcggcgcggc gtggaacgcg gtcacaaccc ccgacgccct gcacgcgttg
    11581 cagctcacgc tgctgatggt ggcggtcgct gtgccgctca acacggtgtt cgggatcgcg
    11641 gcggcgctgc tgctcgtgcg ccggcgcttc cgaggcaagc cgatcctcga ggcaatcatc
    11701 gacctgccgt tcgcggtctc gcccgtggtc gtcggcctcg cgctcgtgct cgtctacggc
    11761 cgcgtcggct ggttcggcaa ctggctaagc gagcacggca tccaggtgat cttctccacg
    11821 tggggcatga tcctcgcgac gatcttcatc acgctgccgt tcgtcgtgcg cgaggtggcg
    11881 ccggtgctgc gcgagatcgg caccgagcag gaggaggccg cacgcacgct gggcgcctcg
    11941 ccgtggcaga ccttcagccg gatcacgctg cccgcgatcc gctggggcgt cggatacggg
    12001 gtggtgctca ccactgcgcg agcgatcggc gagttcggcg cggtcagcgt cgtctcaggc
    12061 cacctcgtgg ggcagacgga gacgctcacc ctgttcgttc aggaccgctt cgagcgcttc
    12121 gacctcgccg gcgcgtacgc cgcgtccgtg gtgctcgcag cgatcgccct ctccactgtc
    12181 gcggtcatga cgctgctcca gaagagaagg ggggcctgat gagcatcgtc gtggtgaacg
    12241 tgtcgaagcg cttcggtgcg ttcaccgcgc tcgacggcgt cgcgctcgag gtgccggacg
    12301 gctcgctcac cgcgctgctg ggaccgagtg gcgggggcaa gtcgacgctg ctgcgtgtga
    12361 tcgccggtct cgaggccccc gacgagggcc gggtgctgat cggcggcagc gacgtgaacg
    12421 ccgtgcccgc ccgcgagcgc ggtgtcggct tcgtgttcca gcactacgcg gccttcaagc
    12481 acatgaccgt ccgcgcgaac gtcgcgttcg gcctcaagat ccgcaagcgc gagaaggcgt
    12541 acatccagcg gcgcgtggac gagctgctgg agctggtcca tctcgagggc ttcgccgatc
    12601 gctacccctc ccagctttcg ggcggtcagc gtcagcgcat ggcgctcgcc cgcgcgctcg
    12661 ccgtcgagcc ccgcgtgctg ctcctcgacg agccgttcgg cgcgctcgac gccacggttc
    12721 gcaaggagct gcgcgagtgg ctccgccgcc tgcacgacga cattcacgtg accacggtgt
    12781 tcgtcacgca cgatcagcag gaggcgatgg aggtggcgga gcagatcgcg gtgctgaacg
    12841 acggtcgcat cgagcagacc gggtctccgc gcgagctcta cgagcagcct gccaacccgt
    12901 tcgtgatgcg cttcgtcggg cccgtgagcg agctcgacgg cgagtggctg cgcccccacg
    12961 acctcgacat cctgctggcg cggtcggaag acgcgatcga ggcgacgatc gagcgcgtcg
    13021 cgtacctcgg cttcgaggtg cgagtcgagc tcgcgctgcc aaacggcgat tcgacctggg
    13081 ttcagaccac ccgtgcccgc gcgggagagc tcgacctgcg cacaggccag aacgtctggc
    13141 tgcaaacggc gcgcacggcg ggaccgcagg cggccccgcc gacgctcacc actgctccgt
    13201 cgcgttagaa gcggctcacc ttgccgacgc gctggaggtg gagcgtccac ctaagcgtgg
    13261 tctgctcctc gccgatcgtg tggttgccgt gcgtgatcgg cttcttcacg atcttctgcc
    13321 cctggatccg cttggacttg gacttgagta gcgcgctgac gttcactccc gcggtggagg
    13381 gctcgaacag gtggccgccc gcctcgtccg tgccgccgct cgcggcgctc gggccaggtc
    13441 atgtgaaaga agtcgtcgtc caggctcatg tcgatcgagc gcttgtgcgc gaggaccgaa
    13501 tagccgccgg gactggtgtg gctgtcgcag ccctcggcgg gcgtcttgaa ctcgccgtgc
    13561 gcgcagtagt cgggaccgct gttggtgtaa ccggaagcgc cgccgatcgt ccgctgcacg
    13621 ttgagcttcg ggttgccaac ctcgatcgag ggttgcccgc ggaacacacg caagacggcc
    13681 ttcgacttgg gcgagctgaa atcgcggcgc tcgctgcccg cgcccttgta cacggtcacg
    13741 cagccgatcg ggtcgacgtc ggtgtagtcg tagctccagc tggtgatctg ggtgccgccg
    13801 agcttgacta gccctgtagc tactcgccgt tggcgagcgc cgccttgacg accgttccgt
    13861 tcgcgtcgta cgtcacgcga aggtactcgc gcccggtgct caggctcccg cgcacgaggc
    13921 cggtgaccca catcgtgcgc gagttgttcg acagccgcgg cacgcccggg ccgagcatct
    13981 gcagcacctc ggttttcgag aggtcgtcaa ggcggtggca gcggagcagg tcgtccgcga
    14041 gctcctggcg gtcggtgggc ggattcacgt tcagcttcgc ctgctgccac ttgaccgggt
    14101 cgaagcggaa gttgcggcag gtgtcgccgc cgccgctctg ggacacgagg gcgccgacga
    14161 ctgtcccgat cacgatgaag ccgacgagcc cggttgcgag gtacacgcgc gcacgacgcg
    14221 agttgaggcg catgccctca ttgaagctgg tgcttccggc tcaccaccac ctgcgctcga
    14281 gcgcgcggtc ggtgccgccc gtctcgtcga gcgcatcgag cgccgccatg tcctcgtcag
    14341 aaagcgaaaa gtcgaagacc tgggcgttct cctcgaggcg ctcgcggtgc gtggacttgg
    14401 ggatcaccgg gaggtcgcgt tcgagcgacc agcggatcag cacctgcgcc ggcgtgcggt
    14461 cgaggcgctc ggcgatctcc gccaccgtcg tgtcgcccag gtggcggccc gtgccgagcg
    14521 ggctgtaagc ctcgagtgcg atgcccttct cctcgcacgc ctcgagaagc ttgcgccgga
    14581 actcgaacgg gctgaactgc gcctggttca cgaccggctg gacctccgcg atgcccatca
    14641 cctcatcgag ctcacccgcg ctgaagttcg acacgccaat cgagcgcgtg tagccggccg
    14701 cgagcgcgcg ctccattccc ggccatgccc aggtcgggcc gccctgcggc caatggatga
    14761 tgtagaggtc gacctgatcc actcccaggc gtcgcaggct gcgctcggcc tcggcctccg
    14821 ggtcctggcg cgcgggatag aacttcgtag tgatgaagat gtcctcgcgc ggcacgccgc
    14881 tctggcggat gccctcgccg acgctctcct cgttgccgta cgcctgcgcc gtgtcgagat
    14941 gtcggtagcc gagctcgagc gcccagcgca ccgcgttcac gcattcggga ccgttgggca
    15001 cctgccacac gcccaggcca agaaggggca tctcgttctg gtcggcaagg acgcgggcgc
    15061 gtccgtccgc ggtcacagct ccggcgggca tggcgggctc ctccgctcgg ggaagaatta
    15121 cggggcccag tgatcttacg gagcggcgtt ggaaacctgg gcggggagga gaaccctccc
    15181 cgcaccacag atcagcgcgc gggcgccggc tccgccatca cgccgagctg gcgaagcatc
    15241 gctgcctcgt cccagtagtt gtgctcctcg cggatcaacc cgtcgtcacc gaactgcatg
    15301 accgagcagc cgtcgatcgt catttgccgg tgcgtggcgg gaagtcccgg aaggtcaccc
    15361 tcgtgggtgc cgctggcgcg ccactcctcg atgccgacgt tgccgtccac ctccacgctg
    15421 attcgctccc actggaggtc agggaacgcg cgcaggtagc cgccgatgta ctcgccgacc
    15481 gcctcccggc cctcgatcgg gccgggcgcg gcctggtcgc gcatgcgcgc atcctcggcg
    15541 taggtggcgg cgacggcgcc gacgtcgtgg cgattgaacg cggcgtcctg ggcgtcgaac
    15601 tggcggatga tctcctcgcg tgtcatgcac gctcctttcg agacgccttc agcgcgagac
    15661 taaccgcggc ggagtcgcga ccgcaaggtc gccggcgagc gggtgacggc tacttcggct
    15721 gcggcacgat gcgtatgtac ggccggggcg attcccagcc ctccgggtat gtgtcgcgcg
    15781 cctcgtcgtc ggagaccgag ccggcgatga tcacgtcgtc accccgctgc cagttcaccg
    15841 gagtcgcgac cttgtgcttc gccgtgagct ggagcgaatc gatcacgcgc agcacctcat
    15901 cgaagttgcg gcccgtcgtc atcgggtaga cgaggatcag cttcaccttc ttgtccggcc
    15961 cgaccacgaa cacgttgcgg acggtctggt tgtcggccgg cgtgcggtcg gccgggtctc
    16021 cggacgtctc ggagggcagc atcccgtaga gcttcgacac gttgaagtcg gagtccgcga
    16081 tcaccgggta gttgggtcgg aagccctgcg tgtcctcgat gtcgtcggcc cactgctggt
    16141 gcttgtcgac agggtcgacc gagaggccga tgatcttcac gttccggctg tcgaagtccg
    16201 gcttgatctt cgccatgtag ccgagctcgg tggtgcacac gggcgtgaag tccttcgggt
    16261 gcgagaacag caccgcccac gagtcgccga tccagtcgtg gaacctgatc gggccctcgc
    16321 tcgtctcggc ctcgaagtcg ggcgccgtgt caccgatctg cagagccatt gccctctcct
    16381 ctcaacgcgc ttcgattgtc ggccggagtc tttcgcagag cgggggtgaa tgtctatcgg
    16441 tatttcggtg atagcccttc gcctgcttcg ctcttcctat gagcccgagc ccttcgcctc
    16501 ggcggtttgt gaggcaccct gcgcgtgacc gggccgcccc gtcgacggct gctccgggcg
    16561 ggcgagcagg tacgccccgg tgctcacagc ggcgaacgcg agtatccgaa gcgcgcccag
    16621 ggctccggac ggcaccggct cgtgaagcac gatcgtgccg gccgctatcg gcaccgcgtt
    16681 ggtgagcagc gtcgcgagcc cggcaaccgt gagcgcgccc cctgcctggt aaccgacctg
    16741 gaggagcgcg gtcccgagcg tgtagcccgc gatgagcgtg aggatgaacg ccgagtgcag
    16801 accgccgtcc gtggcgacct tcgtggacac gtcgccgatc gcgaagaaga gcccaccggc
    16861 aacgccgttc gcgaccgcga tgcccatcac atgccggcca accgtcagcg cgagaaccgc
    16921 cgccgcggcg caggctccga gccacagcga gatgccggcc agcgatccgc cgctgccctc
    16981 ttcgctcccg ccggcgagcg acacggccag cgctaagagc ccgagcaggg acagcagcac
    17041 gccgcgcagc tcacgctggc cgagggggcg gccactcatg cgcgacgaca cgaacgcgag
    17101 cacgccgatg ccgccggcca gaatgctctg cacgagcgcc agctccgcga gcgccagggc
    17161 tgccgcgtag agcaggaagc cggttgtctc catcgcgaag ccgaccagcc agctgcggct
    17221 cgtgagcagg agcctcgcgc cgtggacagg gtggcggagg gtgagcggcg gcagggccga
    17281 cgcggcgtcg tgctcgcgca ggtacgcgat gttcgtcaga gtggtcgagg cgagtgcaag
    17341 cgccagcgct atggcggttt gtgcgctcac gatgtgacgc ttgcaagcat tactcgcgac
    17401 gcacgctgcc caacaagccg cgaaggaggc ccgaagtgat cgtgatgttc agcgcgcgcc
    17461 ggctcaagcc aggcgcctgg gagcagttcc gacaggcatg ggaacccggc gacgccaagc
    17521 cgcccggctt ccagcgcgct taccacgcgc gaaacatccg cgacgaggac gaggtgatct
    17581 cgttcgggct cttcgacatg agcaaggacg attaccaccg ctggcgcgaa gaggcggacg
    17641 cgcaggagac ccaccgcgtg gatcgcatgt cggccttcgt ggagaacgag ttggtttccg
    17701 gcgtgtacga ggtaatcgat gtagccgagt agatgacagc ggtcgccgcg cccgcccaga
    17761 ccgcagtgcc gcagcgccgg gtcctcgtga tcttcggggc gctgatgctg gtgctgctgc
    17821 tggcagcgct cgaccagacg atcgtatcga cggctcttcc gacgatcgtc ggcgacctgg
    17881 gcgggctggc gcacctctcg tgggtggtca gcgcatatct gctggcgcag actgccgtca
    17941 cgccgatcta cggcaagctc ggcgacctct acggccgcaa gcggatcctc caaatcgcgg
    18001 tcgtgctttt cctcgcgggt tccgcgctgt gcggggcgtc gcagaacatg agcgagctga
    18061 tcgcattccg cgcggtccag gggctgggcg gcggcggact gatcgtgctc acgcaggcgc
    18121 tcgtcggcga cgtcgtctcc ccgcgcgagc gcgggaagtt tcagggcctg ttcggggcgg
    18181 tgttcggagt tgcgagcgtc gcggggccgc tgctcggtgg gttcttcgtg gacaacctct
    18241 cctggcgctg gatcttctac atcaacctgc cgctcggcct ggttgccctc gtggtgctcg
    18301 gcatcgtcct gcccgggcgc ggcgaaatcg cgcgtcccaa gatcgactac ctcggcagcg
    18361 gtctacttgc cggagcggtg agctgtgttg tgctcgccac cagccttggc ggcaacactt
    18421 ggccctggga ctccgctcag ctcatcatca cggcctgcgc cggcgtggtt ctcggggtgc
    18481 tcttcgtgct cgtcgagaga acggcgccgg aacccgtcct gccactgcgg ctcttccaga
    18541 accgtacgtt cacgatcgcc gggttgatcg gcttgatcgt gggattcgcg ctctttggct
    18601 cggccgtctt cctgccgctg ttcttccaaa ccgtgaacgg tgtggacccg accacatcgg
    18661 gcctccggct gctcccgctg atagccggct tgcttgtcac gtccatcggg tcaggccagg
    18721 tgatcagccg catcggacgc tacaaaccct tcccgatcgc cggcaccgcc tgcatggtga
    18781 tcgcctttta cttcctatcg aagatggacg ccggcacctc cgcgctcgag gccgcgtggc
    18841 gcctggtcat ccttgggctc gggctcggct tcgtgatgca gattctgatc gtggcggtcc
    18901 agaacgcggt gagctaccgc gacctcggtg tcgccacgtc cgggtcgacc ctcttccgcc
    18961 tgatcggagg ctcgatcggc acggccgtgt tcggggcgat cttctcgaac cggctctcga
    19021 gcgagctcgc cggccgggta cccgcagccg cccagcagca gggccgcctc agcccggcac
    19081 agctgcggca ccttccgccg caggcacacg acgcctacat ccaggcgttc acgaacgcgc
    19141 tcagcgacgt gttcctggtc gcggccgccg ttgccttcct cggcttcctg ctggcgcttg
    19201 cgctgccgga tcagcggctg cgcgacacag tcgaggccgc cgggccgcag gagcactttg
    19261 ccgtcccgcg gggcgacgac acgctgggtg agatcgagcg cgcgctcagc gtgctcgccc
    19321 gcagggacgt gcgcaagcgg ctgtacgagc gcctcgcgcg cgacgctggc atcgaccttc
    19381 cggcgctcga ggcatggacg ctcgcgcgga tctccgaggg cgcccccggt cccgcgaagg
    19441 ggctggcgga ccgtatcggc gtcgagtccg cgcgcgtgtc gcaggcgacc gtggagctgg
    19501 agcgccgggg cctcgtcgtg cgcggcgacg gctggttcgc gtcgacacgg accggggacg
    19561 atctcgtcga gcaactcatc cgccttcgtc gcgagcggct tgcggcgcgc cttgccgact
    19621 gcacgcctga ggagcggcag cagttcgcgc aggtgctcga caggctggcg cgcgacctgc
    19681 tcgcggagcc gccgggcgac cgccagccgg cgagcgcggg gccggtcagc tcgagcgacc
    19741 gctgaccttg ccacgatcgc gtatcgcccg tgcgatgcgc agcggcagaa gcccgaacag
    19801 tggcgcccga cggccgaggt cagcgcggtc ctcgcggtcg aacgcggcgg catagcgccg
    19861 ggcatcggca gcggtggcgt agcgcttcga cgcgctcgcg ccgcaaccgc gctggcatgt
    19921 ccagcgcatt tcgttgccgc tgctcgtgaa gcggaagcgg tggccgaata cgcggcagcg
    19981 gagcatcgtt gactaccgta ggccgccggc cctgtccggc cagcggtcga cttagtagac
    20041 gcgccggata actcgcgtgt gcgcgctagc tccgataaag accactacgg cgttcgtgtc
    20101 gcgcgccgga acagaaagcg gtacgccacg atgaagtagc cgaccgccag cgcgatgccg
    20161 agtgaccacc acctcagccc cacggtgagc gcgtgctcgc tcgacgcggc gttatggatg
    20221 gtcattccga atgggcggtt gtcgtgggcc ggcagcacgt tcggatagag gcccgcagcc
    20281 atcgtcgtga agagcgacga ggcgaagaac gctcgccccc agcgctccat cccctgcagg
    20341 atgaactgcg ccgcgagcgc aaccagggca agcgccggga agatcagccg ccaggggtgg
    20401 tcgccgaagt tggtgaacat ctcgtcgcgc acgtggtagg tgggccaagc cagccccgct
    20461 accagcagta gttcggcgaa ccagaaccct cgcgccagcc gccgagcgcg ttcggcgagc
    20521 ggcggagccg cccgcgccga ccacggctcg gtgatccggt cctggctcgt ggagctcatg
    20581 cacgacgcgt tcgagaacgg tcccgacttc tcggagctcc ccacgtacgt cgaggacacc
    20641 ggagaggtga agtgggtgct cgagtgggcg ctgcgccgag acataccctc gcccgtcatc
    20701 tccgaaagtc aggaggcgct gatggcctac cgcgacctcg agtggccggc ggcgaaggcg
    20761 gtcgcgctgc tcaggcacga gtacggcggg cacccgctgc accgcgccga agagcaattc
    20821 gtgcgaacgt aggctgatgg tcagtcgagc cggggccgcc tcgcgaacgg ccgagctgcg
    20881 ctcgccgagc ggattgctcg aggccacgtt cgtgccgcgg acgggtatgc tcggctggtc
    20941 gctccgcgac ggcgacgacg agctgctcgg gcaccccgtc tcgctggagc gctacgcgga
    21001 gaccggcgag ccgacgggca tcccgctgct tcatccctgg gccaaccggc tcgcctcacc
    21061 ggagtacgag atcgcgggac ggcgcgtggt gctcgacatg aacgcgccga acgtgctcaa
    21121 ggacccgaac ggcctgccac tccacgggct tgcggcggcg tcgccgcact gggaggtgct
    21181 cgacgaggaa ccaccccggc tcagagcgcg gctcgactac gcggcatggc ctgagctcga
    21241 gcgaggcttt ccgtttccgc acctgctcga gctcacggtc accctggcgg acgatcggct
    21301 cgagaccgcg acggagctga tcccgaccgg cgagtcgccg gtgccggtgg ccttcggctt
    21361 ccacccgttc ctgcgcattc ccgggctcga ccgccggcag tggcatgtgg agctaccggt
    21421 gacgagccgc atggtgctcg acgagcgcga gctcccgacg ggtcgcagcg agccggtgca
    21481 catcccgccg ggaccactcg gcgatcgcac cttcgacgac tgcttcgacg gtctcggctc
    21541 gggcgagttc gccgtatccg gggcggggcg gcgacttcac gtccgcttct tggagggtta
    21601 tcgcttcgcg cagctgttcg cgccacccgg cagcgacttc atctgcttcg agccgatgac
    21661 ggcgccagtg aatccgttcg agagcgagca cacgctcctc gccgagccgg tttcgcgcta
    21721 ccgcgcccgc ttcgaggtgg gcgtgacgaa ggtctggctc gggccggcgt ccgtgccgac
    21781 gcgcgtccgc tcggccgtct cggtgatcag ccgaccgccg cggccgcgcg tgaagaagtt
    21841 gtacgaccag cgcaccagaa ccacgagccg gttctcgaag ccgatcaggt agtacaggtg
    21901 gaccacgagc cacgcgagcc aggcgacgaa gccgctgatc ttcacccttc cgaggtcggc
    21961 gacggcgcgc gcccggccga tcgtcgcgag gttgcccttg tcgaagtagc gaaacggcgg
    22021 cgtggagttg ccggcgagcc gatcgcgaat caggcggccg gcgtagcggc cctcctgcat
    22081 cgccaccggt gcgagcccgg gcagcgtgtc cacctgcccg gtgcgtgcgt tgcgcacctt
    22141 gaccatgtcg ccgacggcga gcacgtttgg atgcccgggc agcgtgaggt cggcctccac
    22201 agtgacgcgg cccgcacggt cgcgctcggc tccggacgcc tcggccagga ggccggcaag
    22261 cggtgaagcc gccacgcccg cggcccagat caccgttcgc gtcggcactc gttcggagcg
    22321 cccctccgag tcctggatct ccacgccttc ggaatcgatc ccgacgacgg tgtgccgggc
    22381 aagcggcgtc acgccgatct gaatgagcga gtccgccgcc gcggccgaga gctcggccgg
    22441 gaacgacgga agcacgcggt ccgccgtctc cacgaggagc acccgtccga agcgcggatc
    22501 gatcgccctg aaatcgccgg gcagcgtgtc gcgcgcgagc tccgcgatct ggccggccat
    22561 ctccacgccg gtcgggcctc caccgaccac gacgaacgtg agccaggcgg cgcgagcctc
    22621 cgcgccggga tccagctcag ccgcctcgaa cgcctccagg atccggccgc gcgccacgag
    22681 cgcgctgtcg agcgacttga cctccatggc caccgaccgc cactcctcgt ggccgaagta
    22741 ggagtacgtc gagcctccgg ccaaaaccag cgtgtcgtaa tcgaccgtgc ggggctcgag
    22801 ctcgcccgct cccggcagca ggcgcactcg ctgccgcacc agatcgacct cggtcacctc
    22861 gccgaggacc acgtggacgc tcgggtcgcg gcgaaagatg gtgcgtagtg gctcggctat
    22921 ctcgtcgggc gacagcgcgc cggtggccac ctggtaggtg agcggctgga acaggtggta
    22981 gttgttgc
//
LOCUS       DASVXS010000163         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_326529, whole genome shotgun sequence.
ACCESSION   DASVXS010000163 DASVXS010000000
VERSION     DASVXS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326529"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VF752_09180"
     CDS             complement(<1..1094)
                     /locus_tag="VF752_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8855763.1"
                     /translation="MATAETVIKPDFGSKKKHFVFNEEHDRLRESIGSFVEKEFAPHA
                     EEWERETFPNWVFPRMGELGFLGLSYPEEYGGQGGDYYCNLVLAEEMTKANCGGLAMG
                     IAVHTDMATPPIFLFGTEEQKQSYLVPSIKGEKISCLGITEPDAGSDVAGIRTRAVRD
                     GDDWLINGSKTYITNGHRADYIVLVTKTDPDAGYDGFTLFLVDMDLPGVVREKKLEKL
                     GMHASDTALLAFQDVRVPDEAVLGEVGKGFYHIMWELQGERLIGAAGCVSGAQRAFDM
                     TLKYANERKAFGREIGHFQVIRHKFADMATKIEAARQLVYMTAWRFANGEYPVREISM
                     AKLYASRIAVEVADECIQIHGGAGYMKEYG"
     gene            complement(1507..1905)
                     /locus_tag="VF752_09185"
     CDS             complement(1507..1905)
                     /locus_tag="VF752_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8855764.1"
                     /translation="MSAAVAEGRGATESMLAKINQIRRAHGLRALHQSGSLTRSATRF
                     SRHLMRTNTFGHASHIEASRRFARLGEVLAIHSGRSSAVGWTVSSWMNSPPHRAILLS
                     PAFTSVGFGRTYGRFRGHRANIWVGQLGHR"
     regulatory      complement(1998..2172)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2249..3034
                     /locus_tag="VF752_09190"
     CDS             2249..3034
                     /locus_tag="VF752_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8855765.1"
                     /translation="MDYETILYEVAASGVATITLNQPDTRNALSNELLGELIKAFESA
                     RDDDSVRCVVLASSHEKVFSSGANLSGFSGDVPLVHKHLGSSLFVSLFRLIGELGKPT
                     ICAAGGHVLAGSLGIALACDLIVAKDSATFGTPEIDVGAFPFMIMALIYRNMPRKKTN
                     EMLLLGERISAQEALEAGFVNKVVPAEAFDAAVQEWAEKLAAKSPLIMKLGKDAMFRQ
                     QDMAFVDALEYLHAQLTIALSTEDIVEGVTAFFEKREPEWKGR"
     gene            3038..>3334
                     /locus_tag="VF752_09195"
     CDS             3038..>3334
                     /locus_tag="VF752_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855766.1"
                     /translation="MGVSLIAHHCRTSDRTPGGARGVDALAPLLAKRLGTEPRDIGTP
                     EPARESRWDDDLRDSRGCILEAGGQVDDALSGANVPVLLAADCSIALTTLAAALA"
BASE COUNT          539 a         1139 c         1090 g          566 t
ORIGIN      
        1 atgccgtact ccttcatgta gccggcgccg ccgtggatct ggatgcactc gtcggcgacc
       61 tcgacggcga tccgcgacgc gtagagcttc gccatcgaga tctcgcgcac ggggtactcg
      121 ccgttcgcga agcgccaggc cgtcatatac accagttggc gtgccgcttc gatcttggtg
      181 gccatgtcgg cgaacttgtg ccgaatcacc tggaaatggc cgatttcccg gccgaacgcc
      241 ttgcgctcgt tggcgtactt cagcgtcatg tcgaacgcgc gctgggcgcc cgacacgcag
      301 cccgcggcgc cgatcaggcg ctcgccctgg agctcccaca tgatgtggta gaagcccttg
      361 ccgacctcgc ccaagaccgc ctcgtcgggc acgcgcacgt cctggaacgc gagcagcgcc
      421 gtgtcggagg cgtgcatgcc gagtttctcg agcttcttct cgcgcaccac cccgggcagg
      481 tccatgtcga ccaggaagag cgtgaagccg tcatagcccg cgtccgggtc ggtcttcgtg
      541 acgagcacga tgtagtcggc ccggtggccg ttggtgatgt aggtcttcga gccgttgatc
      601 agccagtcgt cgccgtcacg cactgcacgc gtgcggatgc cggccacgtc ggagcctgcg
      661 tccggttccg tgatgccgag gcacgagatc ttctcgccct tgatcgaagg caccaggtag
      721 ctctgcttct gctcctcggt gccgaacagg aagatcggcg gggtcgccat gtccgtgtgc
      781 accgcgatgc ccatggcgag accaccgcag ttcgccttgg tcatctcctc ggcgaggacg
      841 aggttgcagt agtagtcgcc gccctgtccg ccgtactcct ccgggtagga gagcccgagg
      901 aagccgagct cgcccatgcg ggggaagacc cagttgggga aggtctcccg ctcccactct
      961 tcggcgtgag gcgcgaactc cttctcgacg aacgagccga tcgactcgcg gaggcggtcg
     1021 tgctcctcgt tgaagacgaa gtgcttcttc ttcgacccga agtcgggctt gatgactgtc
     1081 tcggcggtgg ccatgggtcg gcgatctcct ccggttcgcg gtgcatccgg gaccgtaacg
     1141 gaggcgggcc gaaaagtaaa cagtaggact tcctactttc cgcggtggtc gtcgccgagg
     1201 cggcgcgctg gcgcgtgcgc ttacagcagc aatgacggtg gggccgggag cgggagaaac
     1261 gtcccttcca gtccggatcg cgcctcgaga tgtacgaaat acccgcctcg gaggcacaac
     1321 tcctgactct tgttctccta tgcagaacca acctcaggag gtggcgcgca ggcgctcgtc
     1381 caccgtaatt gctgccgttc cgggacgccg gctaagcagc cccgaaccga gcggagcgcg
     1441 ccaaaaagcg cccaaaagaa acggggcccg gccgccccac agccgggccc cagccaaccg
     1501 agcgatctag cggtggccca gctgccccac ccagatattc gctcggtgtc ccctgaagcg
     1561 tccgtaggtg cggccgaagc cgaccgaggt gaacgccggg cttagcagga tcgcccggtg
     1621 tgggggcgag ttcatccacg acgaaacggt ccagcccacg gccgacgagc ggccgctgtg
     1681 gatcgcgagc acctcgccca ggcgggcgaa gcgccggctc gcctcgatgt gcgaggcgtg
     1741 cccgaacgtg ttcgtgcgca tcagatggcg cgagaagcga gtcgcggaac gcgtcaggct
     1801 tccgctctgg tgcagcgccc tgagcccatg ggcgcggcgg atctgattga tcttggccag
     1861 cattgactcc gtcgccccgc ggccctcggc cacggcggca gacattccga aaacagtgag
     1921 cgcgagcgcg cataccaagg cagcgcgaag cggcccgaca agtcctcgag gcatggcggg
     1981 aacagcctcc ttcggtacgt atctctcttg tccgccgacg gggttagctg tcgggctcgc
     2041 gtcaagagag cgacgctacg atcgcggcac tgcgtccgat tcgccccacg ccctgcctct
     2101 cggcttgggc gcttaactgg ttcccccgct cgctctgggt tgccccctca agcgattagg
     2161 cggcgcgcac tatatccact cattcggcgg actgcgccac ttctcctcgt ataactcccg
     2221 ctctctccgg tctgaaagag tgccgcccgt ggattacgag acgatcctgt acgaggtggc
     2281 cgccagcggc gtcgccacca tcaccctcaa ccaaccggac acgcgaaacg cgctctccaa
     2341 cgagctcctt ggcgagctga tcaaggcctt cgagtcggcg cgcgacgacg actccgtgcg
     2401 ctgcgtggtg ctcgcttcct cgcacgagaa ggtgttctcg tcgggcgcga acctctcggg
     2461 cttctccggc gacgtgccgc tcgtgcacaa gcacctcggc tcgtcgctgt tcgtctcgct
     2521 cttccggctg atcggtgagc tcggcaagcc gacaatctgc gcggccggcg gacacgtgct
     2581 cgccggttcg ctcggcatcg cgctcgcttg cgacctgatc gtggccaagg attccgccac
     2641 cttcggcacg cccgagatcg acgtgggtgc cttcccgttc atgatcatgg cgctgatcta
     2701 ccggaacatg cctcgcaaga agacaaacga gatgctcctg ctcggcgagc ggatcagcgc
     2761 gcaggaggcg ctcgaggcgg gcttcgtgaa caaggtcgtg cccgccgagg cgttcgacgc
     2821 cgcggtacag gaatgggcgg agaagctggc ggcgaagtcg ccgctgatca tgaagctcgg
     2881 gaaagacgcg atgttccggc agcaggacat ggcgttcgtg gacgcgctcg agtacctgca
     2941 cgcccagctc acgatcgccc tctcgaccga ggacatcgtc gagggcgtga ccgccttctt
     3001 cgagaagcgc gagcccgaat ggaagggccg ctgagggatg ggcgtctcgc tcatcgccca
     3061 ccactgccgc acgtcggacc gcacgcccgg cggcgcccgc ggcgtggatg cgctcgcgcc
     3121 gcttctcgcg aagcgcctcg gtaccgagcc acgcgacatc ggcactccgg agccggcgcg
     3181 cgaatcgcgc tgggacgacg acctgcgcga ctcgcgcggc tgcatcctcg aggccggcgg
     3241 ccaggtcgac gacgcgctgt cgggcgccaa cgtgccggtg ctgctcgcgg ccgactgctc
     3301 gatcgcgctg accacgctcg ccgcggcgct ggcc
//
LOCUS       DASVXS010000164         5209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_43319, whole genome shotgun sequence.
ACCESSION   DASVXS010000164 DASVXS010000000
VERSION     DASVXS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43319"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1035
                     /locus_tag="VF752_09200"
     CDS             118..1035
                     /locus_tag="VF752_09200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8855767.1"
                     /translation="MRLEAAIVPDRGALAVTGDFFRSREFYDAESVSHTLGVGDSMVA
                     PLVVREIEGEPLHDAVSPYGYPGAVVRAPVAIDDVDWSTSGLVSIFLRDRIGAEPSLR
                     GATLRSSVQIADPSKPLDLREQHRRHIRRNERDGYSARVLEGPASEPEDREQFREAYR
                     ETMVRTEASARYFFDGAWFDTVLSSPLSWLVLVDPPGGGPIAAGAIAVESDRMLHYFL
                     GGTADEHLGASPFKNAVAAMIELASGRGEPLSLGGGVSPGDSLEQFKRGFANSSAPFH
                     TCEIVCDRRAYERLSAGREVGGFFPAYRA"
     gene            complement(1023..2726)
                     /locus_tag="VF752_09205"
     CDS             complement(1023..2726)
                     /locus_tag="VF752_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEX8855768.1"
                     /translation="MFDGPVKRALPTLALALPLALSATPASARDTHAARASASDFELA
                     PAAGQVRRASNGGVMSKPLRAPRRFDLLGMRWRGRSHPDVHVRVRRDGAKWSRWADLG
                     GDPDGGPDPGAREGNVRTTTAPAWSGRSDWVQYRLSRPAAGLRIHFVDAHVRAQKIAT
                     RSQVGAPPMLPRASWGADQCPPRVTPDYGEVKVAFVHHTVSINDYTPDDVPAMILGIC
                     RYHRNSNGWNDIGYNFLVDKFGRIWEGRAGGVDKAVVGAQAQGYNAQSTGIANLGTFS
                     SVPQTDAAIDAMARLIRWKLPLHGQPTAGAVQVTSAGGSSNRYPNGTEVTLQRVSGHR
                     DVDATSCPGDSLYAQLQSLRGKVGNVEPSRPRTKIGAAISAGVLTYGQTARVSGRLQL
                     MKGGAAGGLPVNVQMFISGKWVTIARTTSAGDGSWSVSLKPSAKRIMRAQFPGNGSYV
                     ASSSRQVVVQVRPVLTLSRAAAHATVGDRAILRGRVTPRKSRILLIVRKRVGKVDRKL
                     GTLKLRAKGGRFSASHRLRGPGLYSYQAVFPGDRNSLAASSVALHVRASGSRSGGAYA
                     R"
     gene            complement(2796..3473)
                     /locus_tag="VF752_09210"
     CDS             complement(2796..3473)
                     /locus_tag="VF752_09210"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX8855769.1"
                     /translation="MTSRREQKEALRRQRAEREAAAAAAARRKRRLGIVAATVLVVVA
                     VVAVGAVALTGGNSNGSKAESSVYPKESIPAPKSVGLQDAAKAARCVLRNYPDFGNTH
                     TTAKVTYKTNPPTSGNHNPVPADDGVYTTAPPKENTVHTLEHGRIELQYKPGTPAKVR
                     GQLKALFDEDVYHMVLMPNQTGMKYQVAATAWTHLIGCNQVNDNTWDALRAFKTAYRD
                     QAPERVP"
     gene            complement(3492..4349)
                     /locus_tag="VF752_09215"
     CDS             complement(3492..4349)
                     /locus_tag="VF752_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8855770.1"
                     /translation="MRPHTGRFKHEGHRLAYSEYGEGNPVVLLPGLLFSRQMQEPLAI
                     SIAERGYRVVTMDPLGHGDSDRPQDMWKYSMTAFGREVVALLDHLEIEQAVVGGTSLG
                     ANVTLEAAVLAPDRMRGLVVEMPVLDNALLGCAIAFSPLLVGLTFGAPVMRLVAQLAR
                     RVPRGSNQLIDWGLDWVTQDPGPSAAVLQGLFFGRVAPPREERRTIDAPALVIGHPRD
                     PVHPFSDADMLVRELPNGHLLDASSILELRLTPERLTGEITRFVDECWKPAASNGRKV
                     ASKARVSRA"
     gene            4481..>5209
                     /locus_tag="VF752_09220"
     CDS             4481..>5209
                     /locus_tag="VF752_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8855771.1"
                     /translation="MEASTLALRSAARIPLSRKLLAALSDEKLVAQVRSGSEAAFEVV
                     FDRYHRPILAFCRHMLGTQHEAEDVVQGSFAAAYADLLRSRSEIHLKPWLFTIARNRC
                     LSALRARRESCAEFEPKSLAAGLAEEVERRADLRALVADIAELPEQQRAALVLAELRA
                     LSHPEIAEVLGCEADKVKSLVFQARSTLLDSRKAREMSCDEIRGELATLSRGSLRRNY
                     LRKHLRACLGCREFRDEMKRQHAML"
BASE COUNT          742 a         1797 c         1835 g          835 t
ORIGIN      
        1 gcttcaagca cgagggcacg atgcgcgagc acatcaggcg accggacggc gagctgatcg
       61 actgcgaggt ctacgggctg ctgaagtccg actggaaggg ctacccgtag ctctcccatg
      121 cgtctagagg ccgcgatcgt gccggaccgc ggggcgttgg cggtcacggg cgacttcttc
      181 cgctcgcgcg agttctacga cgccgagagc gtctcgcaca cgctgggtgt gggcgattcg
      241 atggtcgcgc cgctcgtggt gcgcgagatc gagggcgagc cgctgcacga cgcggtgtcg
      301 ccgtacggct atccgggcgc agtcgtgcgt gcgccggtcg cgatcgacga cgtggactgg
      361 tccacgagcg gcctcgtgag catcttcctg agggatcgca tcggcgcgga gccatcgctg
      421 cgcggcgcga cgctccgctc gagcgtgcag atcgccgatc cgtcgaagcc gctcgacctg
      481 cgcgagcaac accggcgcca catccgccgc aacgagcggg acggctattc ggcgagagtg
      541 ctcgagggcc cggcctcgga gcccgaggac cgcgagcagt tccgtgaggc gtaccgcgag
      601 acgatggtgc gcaccgaggc gagcgcccgc tacttcttcg acggcgcctg gttcgacacc
      661 gtgctgtcga gcccgctctc gtggctcgtg ctggtcgacc cgccgggcgg cggtcccatc
      721 gccgccggcg caatcgcggt cgagagcgac cgcatgctcc actacttcct cggcggcacg
      781 gcggacgagc acctcggcgc ttcgccgttc aagaatgccg tcgcggcgat gatcgagctc
      841 gcgtccggcc gcggcgagcc gctcagcctc ggcggcgggg tgagtcccgg tgactcactc
      901 gagcagttca agcgcggatt cgcgaactcg agcgcgccct tccatacctg cgagatcgtg
      961 tgcgatcgca gggcgtatga gcgcctgagc gccgggcgcg aggtgggcgg cttcttcccg
     1021 gcctaccgcg cgtaggcgcc gccgctgcgg ctgccgctcg cgcgtacgtg aagcgccacg
     1081 gagctcgcgg cgaggctgtt ccggtctccc gggaacaccg cttggtagct gtagagcccc
     1141 ggcccgcgca ggcggtgtga tgcgctgaag cggccgccct tcgcgcgcag tttcaaggtg
     1201 ccgagcttgc ggtccacctt gccgacgcgc ttcctcacga tgagcaagat gcggctcttt
     1261 cgcggcgtca cgcggccgcg gagaatcgcc ctgtcgccca cggtcgcgtg ggcggcggca
     1321 cgcgacagcg tcaggacggg acgcacctga accaccacct gccgcgagct cgaagccacg
     1381 tagctgccgt tgccgggaaa ctgcgcccgc atgatgcgct tggcacttgg cttgaggctc
     1441 accgaccagc tgccgtcacc cgcgctcgtg gtccgcgcga tcgtgaccca cttgccggag
     1501 atgaacatct gcacgttcac gggcaggccg cccgcggcgc cgcccttcat caattgcagt
     1561 cgcccgctca cgcgcgccgt ctgcccgtag gtgagcacgc cggcggagat agcggcgccg
     1621 atcttcgtgc gcggcctgct gggctcgacg ttgccgacct tcccgcgcag cgattgcagc
     1681 tgcgcgtaga gcgagtcgcc cgggcaggag gtggcgtcca cgtcgcgatg gcccgacaca
     1741 cgctgcagcg tgacctcggt gccgttcggg tagcggttcg aagagccgcc cgcgcttgtg
     1801 acctgcacgg cgcctgcggt gggctgcccg tgcagcggca gcttccagcg gatcagcctc
     1861 gccatcgcgt cgatcgccgc atccgtctga ggcacgctgc tgaacgtgcc gaggttggcg
     1921 atgcccgtgg actgtgcgtt gtacccctgc gcctgagcgc ccacgaccgc cttgtcgacc
     1981 ccacccgccc ggccctccca gatgcggccg aacttgtcga cgaggaagtt gtagccgatg
     2041 tcgttccacc cgttggagtt gcggtggtaa cggcagatgc cgaggatcat ggccggcacg
     2101 tcgtcgggcg tgtagtcgtt gatcgacacc gtgtggtgca cgaacgcgac cttcacctcg
     2161 ccgtagtccg gggtcacgcg cggcgggcac tggtccgcgc cccaggaggc acgcggaagc
     2221 atcggcggcg cgcctacctg cgatctcgtc gcgatctttt gcgcccgcac gtgggcgtcg
     2281 acgaaatgga tgcgcagacc ggccgccgga cgactgagcc ggtactgcac ccagtccgac
     2341 cggcctgacc aggcaggcgc cgtggtcgtc cgcacgttgc cctcgcgcgc gccggggtcc
     2401 gggccgccgt cgggatcgcc gccgaggtct gcccatcggc tccatttcgc accgtcccgc
     2461 cgcacccgca cgtgaacgtc gggatgcgag cgcccgcgcc agcgcatgcc gagcaggtcg
     2521 aagcgcctcg gcgcgcgcag tggcttcgac atcacgccgc catttgaagc gcggcgcacc
     2581 tgaccggccg ccggggcgag ctcgaagtcg cttgcggagg cgcgtgcggc atgtgtgtca
     2641 cgcgcgctcg cgggcgtggc ggagagcgcc agcggaaggg ccagggccag ggtggggagg
     2701 gcgcgcttca ctggaccgtc gaacaccgtc gcaggcgcgc ggtagcggtg gcgcaggcaa
     2761 acctgcacaa cctggcgctg cagggaatcc tgcggctagg gaacgcgctc gggcgcctgg
     2821 tcgcggtagg cggtcttgaa cgcgcgcagg gcgtcccagg tgttgtcgtt cacctggttg
     2881 caaccgatca ggtgcgtcca cgcggtcgcg gccacctggt acttcatgcc ggtctggttc
     2941 ggcatgagca ccatgtggta gacgtcctcg tcgaacagcg ccttcagctg cccgcgcacc
     3001 ttcgcgggcg tgcccggctt gtactgcagc tcgatgcgtc cgtgctcgag cgtgtgcacg
     3061 gtgttctcct tgggcggcgc cgtggtgtac acgccgtcgt ccgccggaac cggattgtgg
     3121 ttgcccgacg tgggcgggtt cgtcttgtag gtgaccttgg cggtcgtgtg cgtgttgccg
     3181 aagtcggggt agttcctcag cacgcatcgg gccgccttcg cggcgtcttg gaggcccacc
     3241 gacttcggtg ccgggatcga ctccttcgga tagaccgacg actcagcctt cgagccgttg
     3301 ctgttgccgc cggtgagcgc aacggctccc acggcgacga ccgcgacgac cacgagaacg
     3361 gtcgcggcca cgatccccag cctgcgcttg cggcgcgccg cggcggccgc agctgcctcg
     3421 cgctcggccc gctggcgccg aagcgcttcc ttctgctccc tgcggctcgt catccggtca
     3481 gggcaagggt attaggcgcg ggagacgcga gccttcgatg cgaccttgcg gccgttgctc
     3541 gccgcgggtt tccagcattc gtcgacgaaa cgcgtgatct cacctgtgag gcgctccggg
     3601 gtgaggcgca actcgaggat cgagctcgcg tcgagcaggt gcccgttcgg taactcgcgc
     3661 acgagcatgt ccgcgtcgga gaacggatgc accggatcac gcgggtgccc gatcacgaga
     3721 gccggcgcgt caattgtgcg ccgctcctcg cgcggcggcg cgacccgacc gaagaacagg
     3781 ccctgcagta ccgccgcgga cgggccggga tcctgggtca cccagtcgag gccccagtcg
     3841 atcagctgat tgctgccgcg cgggacgcgg cgcgcgagct gcgccaccag ccgcatcacg
     3901 ggcgcgccga aggtcagccc tacgagcagc ggcgaaaacg cgatcgcgca gccgagcagc
     3961 gcgttgtcga gcaccggcat ctcgaccacg agtccgcgca tgcggtccgg cgccagcacc
     4021 gctgcttcga gcgtcacgtt cgcgccgagc gaggtgccgc ctaccaccgc ttgctcgatt
     4081 tcgaggtgat cgagcagcgc gaccacctcg cgcccgaagg cggtcatcga gtacttccac
     4141 atgtcctgtg gccggtccga atcgccgtgg ccgagtgggt ccatggtcac gacgcgatag
     4201 ccgcgctcgg cgatcgagat cgcgagcggc tcctgcatct gccgggagaa cagcagccca
     4261 ggcagcagca caaccgggtt gccctcgccg tactcgctgt aggccagccg gtgcccctcg
     4321 tgcttgaagc ggccggtgtg tggacgcatc ggggcgggag cataaaccgt ccaacccgct
     4381 ggctggttca gcgtttccgg tttaccgtgg aaaccagttc gcccgtaggg ggcggcggcc
     4441 cgcaggcttc cgcctgtgca tggagaggga tttcctgccg atggaagcat cgaccctggc
     4501 gcttcgaagc gccgcgcgca tcccgctgtc gcggaagctc ctcgctgcgc tcagcgacga
     4561 gaagctcgtc gcgcaggttc gctccggcag cgaggccgcg ttcgaggtcg tcttcgatcg
     4621 ctatcaccgc ccgatcctcg ccttctgccg gcacatgctc gggacgcagc acgaggcgga
     4681 agatgtcgtg caggggagtt tcgctgccgc gtacgccgac ctgctgcggt cgcgcagcga
     4741 gatccacctc aagccgtggc tgttcacgat tgcgcgcaac cgctgcctat ccgcgctgcg
     4801 cgcgaggcgc gagtcatgcg ccgaattcga gcccaagtcg ctggcggccg ggctcgccga
     4861 ggaggtggag cggcgagcgg acctgcgggc tctcgtcgct gacatcgcgg agctgcccga
     4921 gcagcagcgc gcggcgctcg tgctcgcgga actgcgcgcg ctgtcgcacc cggagatcgc
     4981 ggaagtgctc ggctgcgagg ccgacaaggt gaagtcgctc gtgttccagg cgcgctccac
     5041 gctgctcgac tcgcgcaagg cgcgtgagat gtcctgcgat gagattcgtg gtgagctggc
     5101 gacgctcagc cgcgggtcgc ttcggcgcaa ctacctgcga aagcacctcc gcgcatgcct
     5161 aggctgtcgc gagttccgcg acgagatgaa gcgccagcac gcgatgctc
//
LOCUS       DASVXS010000165        14847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_186277, whole genome shotgun sequence.
ACCESSION   DASVXS010000165 DASVXS010000000
VERSION     DASVXS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14847
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186277"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VF752_09225"
     CDS             complement(<1..254)
                     /locus_tag="VF752_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8855772.1"
                     /translation="MLAIDAQGVSKRFGDVMALRRVGLWVRDGEIVALTGSNGAGKST
                     LLRVLATTVSPDSGSVSVAGHDALSDPLAVRRKVGVLLPD"
     gene            complement(548..1540)
                     /locus_tag="VF752_09230"
     CDS             complement(548..1540)
                     /locus_tag="VF752_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855773.1"
                     /translation="MTPDNRRLAVGLGTIMLAVFAIVGSNVAANHEPKPHNVPVGIAG
                     QPRVVRAAANAIRRRAPGAFEIHEYASLDAARTAVLHRKIYGALRPEPTPLVLVATAA
                     SPAVSRLLQQASAAAEKRTGQRVAVRDLVPLPSSDSTGATAFSAVLSLIIAGIFGSTL
                     IFVLARERPAHVRVAAMFTLGIGAGLITALVTNVVVGAFSQNFLGVWGIASLYVLAMG
                     LPIIAFQLMVGLAGTAIGALMFLVIGNSASGGSSAPELLPGFWRAVSQLLPPGAATTA
                     MRDVAYFHGHGTAHALVVLALWAVLGAALAITVDWVRGRGALAPARQPTGSGAS"
     gene            1719..2954
                     /locus_tag="VF752_09235"
     CDS             1719..2954
                     /locus_tag="VF752_09235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX8855774.1"
                     /translation="MGTARYVSKAATLRVLFIRPDHIGDLLLTFPAVAALRRVIPAAH
                     LTYAAPADAVAAAERCPSLDAALTVPFPPFGRDGSCDVGWRETVRAEAAKLLGRFDAA
                     LVVRPDDPWSAELVGRASIPIRLGFDMPRTRPHLTDVLPVHDDRHVALDGFDLVDALL
                     ARLSVSARAARVLDPSLVVSRDDEEEVDAVLADEGAHEPLIVLHPGSGWPLKNWPASR
                     WWQLVVELARRTGTRPLLAGTAHEEPLVRRVVEATPAINLAGRLSLGALAALHKRARL
                     VVTTDSGALHLAAAMGAPVVGIFGPGDPFRFAPLAPPSRVRVVRLGLPCSPCGTLEHP
                     PCGAVRDPACVSGITVRPVVEAATELLRAPRPAPRGLPQRTAPPTAPMSDALTHGAAP
                     PVRGGRGETGPLPAAATRR"
     gene            complement(2905..3852)
                     /locus_tag="VF752_09240"
                     /pseudo
     CDS             complement(2905..3852)
                     /locus_tag="VF752_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            3865..4953
                     /locus_tag="VF752_09245"
     CDS             3865..4953
                     /locus_tag="VF752_09245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8855775.1"
                     /translation="MRVVHVAPTPFGQAGIFGGGERYPLELARALSRRVDCELVTFGA
                     PATVREPDGLRVRVLRPVAHLGHPAHVIAPGLPAALRHADIVHTHHTRSAPSRIAAVT
                     ARACRGVAVTTDHGLPGGTWRGGLLALFHRFLMVSEYSARELGSPPSRTRIVYGGVDC
                     ARYRPDPDRSRRGVLFVGRLTPHKGVDRLIRALPERAELTIVGTAGHDPNPPERDYPQ
                     LLRKLAHGRPVRFLGAVPDDELAHLYRQAAVVALPSVDLTCYGRHIGIVELLGLAALE
                     AMASGTPVVCSRIGGLPEIVDDGITGVLVEPGDLDGLRAALDELLRDRALAVRLGRNA
                     RDRALERFTWDSCATRCLAAYEEILGVA"
     gene            complement(4950..5330)
                     /locus_tag="VF752_09250"
     CDS             complement(4950..5330)
                     /locus_tag="VF752_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX8855776.1"
                     /translation="MSFEVGASRSLRALHRLPWGTEAERQPHAHDYRIEVVVERERLD
                     ERGVVCDLDVLRAGLDDVLGRLEGRDLDEIRPPDADGVTVEILARWLHGSLMEVVGRA
                     GGETLAIRVWEAPDAFGGYRACVI"
     gene            complement(5327..6322)
                     /locus_tag="VF752_09255"
     CDS             complement(5327..6322)
                     /locus_tag="VF752_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEX8855777.1"
                     /translation="MNARAVQFVAPGRVELRDVEVAEPASGELLVRTEFSGISGGSEM
                     LAYRGEVDPEVALDSTLEALGGTFAYPFAYGYSTVGRVERSRGSIHEGARVFAFHPHQ
                     DLFVVGEGDVIPLDDEDPRRATLLPLVETALQVSLDAGPLFGEIVAVLGLGCVGILAG
                     VLMGRAGACVVGSEPQPERRRAAARLGVDSVAPEELAARLNDVTHGRGVPLVIEASGR
                     PEVLAAALPLLAQEGTALVCSWYGTKPVQLPLGADFHRRRLVIRSTQVSTIPSKLATR
                     WDRRRRREVSRVLLSELPLEPLATHEFPFERAPDAYAAVAGGTRGLIHAALRYEA"
     gene            6499..7815
                     /gene="ribA"
                     /locus_tag="VF752_09260"
     CDS             6499..7815
                     /gene="ribA"
                     /locus_tag="VF752_09260"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HEX8855778.1"
                     /translation="MRGVAQEVVRVPLPTPAGEFDLRAFACSRDSIYLVLVRGDLGDG
                     RGVLTRLHSECLTGDALGSLRCDCGIQLRAALRAIASEGRGVLIYATGHEGRGIGLIN
                     KLRAYVAEDDGLDTLEANRHLGLPTDAREYDDAAACLGALGVRTIRLLTNNPHKATSL
                     RRAGVQIDDVVALATSPHARNLHYLRSKELRRGHVRPTGDRLENEVPKALDVSGLLGS
                     VASRDSRPYVVLKYAQTLDGRIATRTGDSKWISGEPERRASHALRAACDAVLVGIGTV
                     VADDPQLTVRLVPGASPLRVVLDSTLRIPATAQILSDAAPTLVITSALSRTEHRDALR
                     ERGIGIQVVHAEPPWGVDLGAALSLLHGAGFRSLLVEGGAAVITSFLRTRLADRVVVG
                     IAPTIVGTGKEAVGDLSIARVSDGLRLAGRSVHLLGDDVVMAGDVI"
     gene            8418..9371
                     /locus_tag="VF752_09265"
     CDS             8418..9371
                     /locus_tag="VF752_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8855779.1"
                     /translation="MKVFVAGATGVLGRALLPELVARGHEVVGMTRSASKQDLVRSLG
                     AHSVVADALAPDAVAQAVALAEPEVIVHQLTALSGKISIRDARHPERSSGAIMTNRLR
                     TVATDHLLAAGRAVGARRFVAQSFGAFRFAGTGGPVQTEADSLEPDPRQTGLEAILYL
                     ERAVTTIEWGEGLALRYGGFYGPGTGISAAPDAVMAGPIRKRRFPIVGDGGGVWSLVH
                     IEDAAAATAIAVERGQPGIYNVVDDEPAPVRELLSVLASALGAKPPRRIPRWLGRLAG
                     GEMATLMMTEARGASNEKAKRELGWQLRYPSWRQGFAQGLD"
     gene            9531..10190
                     /locus_tag="VF752_09270"
     CDS             9531..10190
                     /locus_tag="VF752_09270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8855780.1"
                     /translation="MNDQPERTSPPPPLPVRVIGKALTRMVARSPWTWPLLRRLVRGF
                     FDRSADGWDERVRPDAPDHLAALAAAVARLDAAPARILDLGTGTGAGALWLAREFPEA
                     RVTGVDISQAMIARAKAKRPDELSGRVEFLVGDAERLPFAGGSLDLVAQISVPVFFDE
                     VARVLAPRGYVVVVSSLGLKTPFHTPERTVRRGFRGRGIETVATGAEGPGTFFLGRRS
                     A"
     gene            complement(10289..11215)
                     /locus_tag="VF752_09275"
     CDS             complement(10289..11215)
                     /locus_tag="VF752_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8855781.1"
                     /translation="MSVNQVIAETASPFEAGTIATERAPIFARRGGSGPPLLLLHGFP
                     QTHLMWRDVAALLVRDFTVVCADLPGYGQSGCPPSGADHTAYAKRRMARDMVEVMDAL
                     GFERFCIAGHDRGGRVAYRTALDHPERVERLAVLDVLPIDITWERANARLALEFWPWS
                     LLAQPEPLPERLVGAAPDAVIDDALGGAWGSPAEAFDDEVRKAYIAALRDPDHVHAIC
                     EEYRAAATIDREHDAADRRAGRRITCPTLSLWSGRGALSTWYADTGGPLALWRDLADD
                     VDGRPVDGGHFFPEEHPTDTATALRAFFSSGP"
     gene            11339..11911
                     /locus_tag="VF752_09280"
     CDS             11339..11911
                     /locus_tag="VF752_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8855782.1"
                     /translation="MTKIVVSDNVTLDGVIQDPAGDEGFRVGGWVGLIKDRPELGKLA
                     LDEALGAEALLLGRRSYEWLAARWPSRSGELADRLNSLPKYVVSSTLDDPDWNNSTVL
                     KGNVIDEISKLKQKLDGDIVVPASFQLVRTLIEHDLVDELRLKLFPVVLGAGERLFGE
                     TSDKKPMRLINIQTVEGDVAFLTYELVRKA"
     gene            complement(12000..14147)
                     /locus_tag="VF752_09285"
     CDS             complement(12000..14147)
                     /locus_tag="VF752_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8855783.1"
                     /translation="MTRGLAVLGDIAYRHRGRVVLAWIGAAILIIGAGSAAKGAYNAD
                     YNTPGSDSKAASEITKQRFAGYSGQEISVVWRDHAGATSPAAKRRLNAFFAQVEKVKH
                     VSAHTPTRVSRDGTIGATTLPLTVPGWEVKTSQGKQLIEAANENSGGGVEIRLGGDPI
                     HDAEGQASPEGIGFVGAAIVLLIAFGSLVAAGLPLVIALVGLGISSAGLIALLANVID
                     VPDWTTAVSGLIGIGVGIDYALLVLTRFRSAMRDGKDRHDAVVEAVTHAGRSVIIAGC
                     TVVIAVLGLCLTGLKYMYGVAISTSLAVLVVMIASVTLLPALLAFLGPRVDRLRIPLA
                     GRSLGTGSGRESSAARWSHAVQRRPWPAAVAATAVLLALAAPALGMRLGFPDAGNDPP
                     GKMTRQAYDLNTHGFGPGSNGPVVIAGTLPGAAAKPRIESFAARLRSQPGVAFVTRPV
                     INTAGNAALITLIPTTSPQDKATADLVKRLRDKVVPSALAGSGVTVKIGGVTAALEDQ
                     SDYVTRHMPSFISGVVGLSFLLLLVAFHSPLIALKAAVMNLLSVGAAYGVVTLAANGG
                     GLSNLIGIDHEVPVAPFMPVMMFAILFGLSMDYEVFLISRIREEYLKDGDTRRAVADG
                     LAKTARVITAAAAIMVVVFLAFLASQEVFLKLLGIGLASAIFLDATLVRLVLVPAVMQ
                     LLGKGNWWIPNWLERVLPTLGVETLTPSAQARS"
     gene            complement(14270..>14847)
                     /locus_tag="VF752_09290"
     CDS             complement(14270..>14847)
                     /locus_tag="VF752_09290"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8855784.1"
                     /translation="ESLSPEQRAVLLLHDVFDYGYPEIAPIVGKSEDNVRQLATRARR
                     HVEQRRPRFQTTREQRDELARRFFQAAEQGDLAGLEALLAHDVELTGDGGGKVPALAR
                     TLRGRNRVARTLINWFRLGALLPGVSFRPVEVNGGPGALLVDAQQRLIGVLALEIAGG
                     QITSISAIVNPDKLTHLGPVGDFGSLLGSAR"
BASE COUNT         2306 a         5141 c         5196 g         2204 t
ORIGIN      
        1 tcgtccggca gcaggacgcc aaccttgcgc cgtaccgcga gcgggtcgct cagcgcgtcg
       61 tgtccggcga cgctgacgct ccccgaatcg gggctcaccg tagtcgcgag aacgcgaagc
      121 agcgtgctct tgccggcgcc gttcgagccg gtcagggcga cgatctcgcc atccctcacc
      181 cacaggccga cgcggcgcag cgccatcacg tcgccgaagc gcttgcttac tccttgagca
      241 tcgattgcga gcatcggttc gcggtcgttc cttgacccac ttccgtttac ggcgtcagtg
      301 cgaggttcct tctggtccgg tgactagatg cgcatgcaag atccgcccga cctcaaatcg
      361 ccccgaagtc ggctctggcc cgaaatccac cctaagagcg accccgcagg cgcgcaacct
      421 aaagaaggct tgcgtgcatc gcgatcgagg acatccagct gccgtgtttg cgccgaggcc
      481 tgtcgccgat gtgaagcgaa gcgggttgcg cgagagaggc gcgtggtggc ggtcgtcagg
      541 cgcgccctca ggaagcaccc gagccggtcg gctgacgagc gggagccagc gcgccgcgcc
      601 cgcggaccca gtccacggtg attgccagcg ccgcgccaag taccgcccat agggcgagca
      661 cgaccagcgc gtgcgcagtg ccgtggccgt ggaagtaggc gacgtcgcgc atggcggtgg
      721 ttgctgcgcc cggcggcaac agctggctga ccgctcgcca gaagccgggc agcagctccg
      781 gcgcggagct gccgcccgat gccgagttgc cgatcacgag gaacatgagc gcgccgatgg
      841 cggtcccggc gaggccgacc atcaattgga acgcgatgat cggcagcccc atcgcgagga
      901 cgtacagcga ggcgatgccc cagacgccga gaaagttctg agagaacgcg cctaccacga
      961 cgttcgtcac gagggccgtg atcaggccgg cgccgattcc cagggtgaac atcgcggcga
     1021 cgcgcacgtg cgcgggacgt tcgcgcgcga gcacgaagat cagggtcgag ccgaagattc
     1081 ccgcgatgat gaggctcagg acggcggaga acgcggtcgc cccggttgag tcggaacttg
     1141 gcagcggcac gaggtcgcga acggcgacgc gctgaccggt cctcttctca gcggcggccg
     1201 acgcctgctg gaggagccgg ctaacggcgg ggctcgcggc ggtcgcgacg agcacgagcg
     1261 gggtcggctc gggccggagc gcgccgtaga tcttgcggtg cagcaccgcg gtgcgcgcag
     1321 cgtcgagcga cgcgtactcg tgtatctcaa acgcgccggg agctctgcgg cgaatcgcgt
     1381 tggccgcggc gcgcacgacc ctcggctgtc ccgcgatccc gaccgggacg ttgtgcggct
     1441 tcggctcgtg attggccgcc acattcgagc cgacgatcgc gaagacggcg agcatgatgg
     1501 tgcctagccc gaccgccagc ctacggttgt ccggcgtcac gagggcgatc ctcccaccgg
     1561 ggccgcgaag cgagcaaggt cggcccagtg gcggcgatac gtcggccggt gccgacgtta
     1621 ggtgccttct cacgcgagat tgggtagtgg ggctcggaaa ccaattcggc ctcccggatt
     1681 cccgacccgc gaccggcgtc gctccaccac cgacggcaat ggggacagct cgttacgtat
     1741 cgaaggcggc gacgctgcgg gttctgttca ttagaccgga ccacatcggg gacctcctgc
     1801 ttacctttcc ggcggtcgcc gcgctgcgac gcgtgatccc cgccgctcac ttgacgtacg
     1861 cggcgccggc ggacgcggtg gctgccgcgg aacgatgtcc gagcctcgac gccgccctga
     1921 cggtgccgtt cccgcccttc ggtcgcgacg gatcatgcga cgtaggctgg cgcgaaaccg
     1981 tgcgcgctga agcagcgaag ctcctcgggc ggttcgacgc agcgctggtc gtgcgcccag
     2041 acgacccgtg gtcggcggag ctcgtgggcc gcgcgtctat accgatccgg ctcggattcg
     2101 acatgccgcg cacgcggccg cacttgaccg acgtgctccc agtgcacgac gaccgccatg
     2161 tcgccctcga cgggttcgac ctcgtggacg cactgctcgc gcggctttcc gtgagcgcgc
     2221 gcgccgctcg cgtgctcgac ccatcgctcg tcgtgtcgcg cgacgatgaa gaagaggtcg
     2281 acgccgtgct cgccgatgaa ggcgcgcacg aaccgctcat cgtgttgcat cccggctccg
     2341 gctggccgct caagaactgg ccggcgtcac gctggtggca gctggtggtg gagctcgctc
     2401 ggcgcaccgg tacgcggccg ttgctggccg ggaccgcgca cgaggagccg ctcgtccgcc
     2461 gggtcgtcga ggcgacgccc gcgatcaacc ttgccgggcg tctctcgctc ggagctctag
     2521 cggcgcttca caagcgggcg cggctcgtcg tgacgactga cagcggcgcg ctgcatctcg
     2581 ccgccgcgat gggcgcaccc gtcgtgggca tcttcggtcc cggcgatcca ttccgcttcg
     2641 cgccgcttgc cccgccctcg cgcgtccggg tggtgcggct cgggctaccg tgcagcccat
     2701 gcgggacgct cgagcacccg ccgtgcggcg cggtgcgcga ccctgcctgc gtcagcggga
     2761 tcaccgtccg gcccgtcgta gaagcggcta ccgaactcct gcgggcgccg cgtccagcgc
     2821 cccgcggatt gccgcaaaga acagcgccgc cgactgctcc catgtcggac gcgctgacgc
     2881 acggcgctgc gccgcctgtc cgaggcggtc gcggagagac gggtccgctg ccagcagccg
     2941 caacgcggcg ctgaggccct ggatgtcacc tgggggcaca agtagcccct cgtgtccgtc
     3001 gtctgcgagg tacggcagat tccccgcgcg ccagccgatc accggtagac cgaacgccat
     3061 cgcctcgccc caaacggtgc cgtagggctc gcggaaggcc ggcagtacga acgcatcggc
     3121 acccgcgtac agggcagcca ccgcttcgcg cgggagcgcg ccatgtacga ccacccgcgc
     3181 ggccagatca gggcttgcaa gccgccgccg cacacgcgcg gcgtacaccg gatcggcctc
     3241 cgggtcgcct gcgagatgaa gcgtcgccat gtcgggcgcc agtccggcga acgcctcgag
     3301 cagctcgagg atccccttac gctgaatcca gttggcaaca cagaggaagg ccgcgccgcg
     3361 ccctgcgcgt aggtccggct gcgcagccgc ggccgtggcc acgtcgcggc cgggtggtac
     3421 cacgcgtatg cgctcgcctc cgaagcccgc cgcggcgacc tggtccgcca gcagctcgct
     3481 cgcaacgatc agcagccgcg cgcgcctgta ggccacgcgg tcaaggggcg cctgaagccg
     3541 cctgcggacc cgaccgtggt cgatgccgcc cggtggctgg tggagcacgc cgatcaacgg
     3601 gacagcgggc gagcgcgcag caagccacgg gccggcgaac gtcgcggcga tgctgtcgag
     3661 caggatcgcg cgcgcggaga gccggcggac atggctgaac acacgtgctg cggccagcgc
     3721 cggcagcgcg aacgggcgct ccgggaacga taagaagacg atccgagccc cgtgcacggg
     3781 ggccgctgct gccatccgca ggtggtagag ataaccgccg gtcagccggc gagggtcgcc
     3841 gagcgtgacc atgcagagca agagatgcgg gtcgttcacg tcgcgcccac gccattcggt
     3901 caggccggga tcttcggcgg cggtgagcgc tatcccctcg agctcgcgcg cgcgctctcc
     3961 cgccgcgtgg actgcgagct ggtcacgttt ggagcgcccg cgacggtccg cgagccggac
     4021 ggtcttcgcg tgcgggtcct tcgcccagtt gcacatcttg gccaccccgc tcacgtgatc
     4081 gcccccggcc tgcccgccgc gttgcgtcac gctgacatcg ttcacaccca tcacacgcgg
     4141 agcgcgccga gccggatcgc cgcggtcacc gcgcgggcct gccgtggcgt ggcggtcacg
     4201 acggaccacg gattgccagg cgggacgtgg cgaggcgggc tgctggcgct gttccatcgc
     4261 ttcctcatgg tgtccgagta ttccgcgcgc gaactaggct cgccgccgtc ccgcacgcgc
     4321 atcgtctatg gcggcgtcga ctgcgcccgg taccgtcccg accccgaccg ttcgcgccgt
     4381 ggcgtcctgt tcgttggccg cctgacgcca cacaaaggcg tcgatcgcct catccgtgcg
     4441 ctgcccgagc gggccgagct cacgattgtg ggtaccgccg ggcacgaccc caacccgccg
     4501 gagcgggact acccgcagct actccgcaag cttgcgcatg ggcgaccggt ccggttccta
     4561 ggcgccgtac cggacgacga actcgcgcac ctctaccggc aggcggcggt agttgcgctg
     4621 ccgtcggtcg acctgacctg ctacgggcgt catatcggca tagtcgagtt gcttgggttg
     4681 gccgctctgg aggcgatggc cagcgggacg ccggtggtct gcagccggat cggcgggttg
     4741 cccgagatcg tcgacgacgg gatcacgggg gtgctcgtcg agccgggcga cctcgacggc
     4801 ctgcgcgctg ccctggacga actactacgc gaccgcgcac tcgcggtgcg gcttgggcga
     4861 aacgcgcggg atcgcgcgct cgagcgcttc acctgggatt cgtgcgcaac gcgctgcctc
     4921 gcggcgtacg aggaaatcct gggcgtcgct tagatcacgc acgcgcggta gccgccgaag
     4981 gcgtccggag cctcccacac tcgaatcgcc agcgtctcgc cgccggcgcg ccccacgact
     5041 tccatgagcg acccgtgcag ccagcgggcg aggatctcaa ccgtcacgcc gtcggcgtcc
     5101 ggcgggcgga tctcgtcgag atcccggccc tcgagccgcc cgagcacgtc gtcgagcccc
     5161 gcgcggagca cgtccagatc gcagacgaca ccgcgctcgt ccaggcgctc gcgctcgaca
     5221 accacctcga tgcgatagtc gtgagcgtgc ggctggcgct cggcctcggt accccacggc
     5281 agcctgtgca gcgcgcgcag cgaccgcgac gctccgactt cgaagctcat gcctcgtacc
     5341 ttagggcggc gtggatgagg ccgcgcgtac cgcctgccac cgcggcgtag gcgtcaggcg
     5401 cgcgctcgaa cgggaattcg tgggtcgcga gcggctcgag cggaagctcc gagagcagca
     5461 cacgggagac ctcgcgccgc cgccggcggt cccagcgagt ggcgagcttc gacgggatcg
     5521 tcgacacctg cgtgcttcgg atgaccagcc ggcggcggtg aaagtcggcg cccagcggaa
     5581 gctggaccgg cttggttccg taccaggagc acaccagcgc cgtcccttcc tgagcgagca
     5641 atggcagggc ggccgcgagc acctcgggcc gcccgctcgc ctcgatcacg agcggcacgc
     5701 cgcgcccgtg tgtgacgtca ttcaggcgcg ccgccagctc ctccggggcg accgaatcga
     5761 ctccgagacg ggcggcggct cgccgccgct ctggctgcgg ctcgctaccc accacgcacg
     5821 cgccggcacg gcccatcagc acgccggcaa ggattcccac acagccaagc ccgagcaccg
     5881 ccacgatctc cccgaacagg ggaccggcgt cgagcgagac ctgtagcgcg gtctcgacca
     5941 gcggcagcag cgttgcgcgc cgcggatcct cgtcgtcaag cgggatcacg tcgccctcac
     6001 cgactacgaa caagtcctgg tggggatgga acgcgaatac gcgcgcgccc tcgtggatgc
     6061 tgccgcgcga gcgttcgacg cgtccgacgg tgctgtagcc gtaggcgaac gggtaagcga
     6121 acgtgccacc cagcgcctcg agcgtgctgt cgagagcaac ctcggggtcg acttctccgc
     6181 gataggccag catctcactg ccgccgctga tcccggagaa ctctgtgcgt accagcagct
     6241 cgccactggc gggctcagct acttcgacat cccgaagctc gacacgccca ggcgcaacga
     6301 actggacggc tctcgcgttc atcccacgat gccctcgcgt gagagcgcga acatcaggtt
     6361 cgtcccgatc tgctgtgact ggtcagccct gtgggggtat gctcgtgccg ccgcgggcag
     6421 ctggaaccga ccccgggagg actccacgtg agccgcggga aaggacatcg ggcaccgccc
     6481 gggagcctat tacgcagcgt gcgcggcgtc gctcaggagg tcgtgcgcgt cccgctgccc
     6541 acaccggcag gcgagttcga ccttcgggcg ttcgcgtgct cgcgggactc catctacctg
     6601 gtgctcgtcc gaggcgatct cggtgacgga cgcggcgtgt tgacccgcct tcactctgag
     6661 tgcctcaccg gggacgcact cgggtcgcta cgttgcgact gcggcatcca gctgcgcgca
     6721 gccctgcgag cgatcgcatc cgagggacgc ggcgtactga tctacgcgac cggccacgag
     6781 gggcgcggca tcggcctgat caacaagctg cgggcgtacg tcgccgaaga cgacggcctc
     6841 gacacgctcg aggccaacag gcacctcggg cttccaaccg acgcgcgcga gtacgacgac
     6901 gcggccgcct gcctcggcgc cctgggggtc cgcacgatac ggctcctcac gaacaacccg
     6961 cacaaggcga caagccttcg acgcgccggc gtacagatcg acgacgtcgt agccctggcg
     7021 acgtcgcccc acgcccgcaa cctccactat ctccgctcga aggaactgcg gaggggccac
     7081 gttcggccga ccggggaccg tctcgaaaac gaggtgccga aggcgctcga cgtcagtggg
     7141 ctgctcggct cggtcgcctc tcgggactcg cggccctacg tggttctgaa gtacgcgcaa
     7201 accctcgacg ggcggatcgc gacgcgcacc ggcgattcaa aatggatcag cggagagccc
     7261 gaacgccgcg cctcgcacgc gctgcgcgcc gcctgcgacg ccgtcctggt cggcatcggc
     7321 acggtggtcg cggatgaccc ccaactcacc gtccggctgg tgcccggcgc ctcgcccctt
     7381 cgcgtcgtgc ttgactcgac gctgcgcatc cccgccactg cgcaaattct ctcggacgcc
     7441 gctccgaccc tcgtcatcac gagcgcgtta tcacgcaccg agcaccggga tgcgctgcgc
     7501 gagcgcggca tcggcattca ggtcgttcac gccgagccgc cctggggagt ggatctcggt
     7561 gcggcactgt cgctgctgca cggggcgggg ttccgctcgc tgctcgtcga gggcggcgcg
     7621 gccgtcatca cgtcgttctt acgcacgcgt ttggcggacc gagtcgtcgt tgggatcgcc
     7681 ccaaccatcg tcggaacagg taaggaagcg gtaggcgacc tgagcatcgc ccgcgtgtcc
     7741 gatggcctac gcctggccgg acgatcggtg catctcctgg gcgacgatgt cgtcatggcc
     7801 ggagacgtca tctgatcgaa gcacggcgcg cgtcacggga tgtgtataag cggctggaat
     7861 gtcgctactg gacaggatcg gggcgacgcg gccgggcacg tggacgatcg cgcgcgtcgt
     7921 gtcacccttg cagcgacgac tgctggccgc aacccgtggt cgcgtgtcgc tcacgggaaa
     7981 acgaccggtc ctcttgctgc gcttctcagc cgtacgcccg cgtagcgatg cctgggtaag
     8041 cgagcacgaa ccccgcgcga gcgtgtccgg ctcgagccgg tagcgcaact cgtaagaacc
     8101 cctagctgag taccggccgc ggtgagctcg tgcgcgccgg gctcgacgtg tgctacctcc
     8161 atccgtgggg ccccgtcacc aatccacgca agagacatcg gcattccgcg aacactacgg
     8221 acgtgcagtt ggctacaggc aacggatacg gcacccgctt tccttggcga gcgatattca
     8281 ggatcgccgc tgatcgcgtc gccgcttagg acgacctcag aagccgcatc gccggcgccg
     8341 gtcgttcctc cgtgagcgct gtcacaggcg gcgcctctat ctcgtctcag agagcaaatg
     8401 agacgagagc agcagtcatg aaggtcttcg ttgccggcgc cacaggcgtt ctcggcagag
     8461 cgctcctccc tgagctcgtg gcgcgaggcc atgaggtggt cgggatgacc aggagcgcgt
     8521 cgaagcagga cctggtgcgc agcctgggag cgcactcggt cgtggccgac gctctcgccc
     8581 ccgacgcggt cgcccaggcg gtggcgttgg ccgagccgga ggtgatcgtg catcagctca
     8641 cggcgttgtc cgggaagatc agcatccggg acgcgcggca ccccgagcgc tccagcgggg
     8701 cgatcatgac caaccggctg cgtaccgtgg cgacggacca tctcttggcc gccggccgcg
     8761 ccgtcggcgc gcggcgcttc gtggcgcaga gctttggggc tttccggttc gccggcacgg
     8821 gcgggccggt gcagaccgag gccgattccc tcgagccaga ccctcggcag acgggcctgg
     8881 aggcgatcct ctatctggag cgggcggtga cgacgatcga gtggggagag ggcctcgcat
     8941 tgcgctacgg cggcttctac ggcccgggga ccgggatcag cgcggctccg gatgcggtga
     9001 tggccgggcc gatccgcaag cgtcggttcc cgatcgtcgg cgacggtggt ggcgtctggt
     9061 cgctcgtcca catcgaagac gcagcggcgg cgaccgccat cgcggtcgag cgcggccagc
     9121 cgggtatcta caacgtcgtc gacgacgagc ccgcgccggt gcgggagttg ctgtcagtgc
     9181 tggcgagcgc gctgggtgcc aagccaccaa ggcgcatccc gcgctggctg gggcggctgg
     9241 cgggcggtga gatggcaacg ctcatgatga ccgaggcgcg gggtgcctcc aacgagaagg
     9301 cgaagcgcga gctcggctgg cagctccgat atccgagctg gcggcagggc ttcgcgcagg
     9361 gactcgacta gccagatagg cggggccata gttgctctcg cactcgcaag tcgagacgcg
     9421 gcaatcgact cggcgccgtc cgaccagcgc gcctcgttgg ccgtcggggg tcgctcgtcg
     9481 aggccgagga tccctcagca gccggcgtgc tgccgatcta gggttgcgac atgaatgacc
     9541 agcccgagcg caccagtccg ccgccgccgc tgccggtccg cgttatcggc aaggcgctga
     9601 cgaggatggt ggcgcgctcc ccctggacgt ggccgctgct gcggcgcttg gtgcgcggtt
     9661 tcttcgaccg ctcggccgac ggctgggacg agcgcgtgcg gccggacgcg ccggaccacc
     9721 tggcggcgct cgccgccgcg gtcgcccgtc tggatgcggc cccggcgcgg atcctcgatc
     9781 tcggcaccgg aacgggcgcg ggagcgctgt ggctcgcgcg agagtttccg gaagcgcgcg
     9841 tgacgggcgt ggacatctcg caggcgatga tcgcgcgggc gaaggcgaag cgcccggacg
     9901 agctgtccgg gcgcgtcgag ttcctggtcg gcgacgccga gcggctgccc ttcgcgggcg
     9961 gctccctgga tttggtggct cagatcagtg tgccggtctt cttcgacgag gtggcgcgcg
    10021 tgctcgcccc gcgcggctat gtggtcgttg tcagcagcct tgggctaaag acgccgttcc
    10081 acacgcccga gcgcaccgtg cgcaggggct tccgcgggcg cggtatagag accgtcgcga
    10141 ccggggcaga gggcccaggc acgttcttcc tgggcaggcg ctcggcctga agcgaagacc
    10201 gggcgacgga accctccacg ttggcgccgc gaacggagcg acgctcgcca gcgtcaccca
    10261 ggggaagcgc cgtcacgaac gaatccctct aaggcccgct cgagaagaac gctctgagtg
    10321 ccgtggctgt gtcggtgggg tgctcttcgg ggaagaagtg tccgccgtcg acgggtcggc
    10381 cgtcgacgtc gtcggcgagg tcgcgccaca gcgccaatgg cccgcccgtg tcggcgtacc
    10441 aggtcgagag cgccccgcgg ccgctccaga gcgacagcgt cgggcaggta atgcggcggc
    10501 ccgcgcggcg atcggcggcg tcgtgctcgc ggtcgatggt tgctgcggcc cggtactcct
    10561 cgcagatagc gtggacgtga tcggggtcgc gcagcgcggc gatgtaggcc ttgcggacct
    10621 catcatcgaa ggcctccgcc gggctgcccc atgcgccgcc caacgcgtca tcgatcacgg
    10681 cgtcgggtgc cgcgcccact aggcgctcgg gaagcggttc gggctgagcg agcagcgacc
    10741 agggccagaa ctccagcgcc agccgggcat tcgcccgctc ccacgtgatg tcgatgggga
    10801 gcacatcgag caccgcgaga cgctccaccc tttcgggatg gtccagcgct gtccgatagg
    10861 cgacccggcc accgcggtcg tggccggcga tgcagaagcg ctcaaacccg agcgcgtcca
    10921 tgacctcgac catgtctctc gccatgcgtc gcttggcgta ggcagtgtga tccgcgcctg
    10981 agggcgggca gccgctctga ccgtatccgg gcaggtcggc gcacaccacc gtgaagtcac
    11041 gcacgagcag agccgcgacg tcgcgccaca tcaggtgcgt ctgcggaaag ccatgaagga
    11101 gcagcaacgg tggcccagag ccaccacgac gtgcgaagat cggcgcccgc tcggttgcga
    11161 tcgtgccggc ctcgaagggt gacgcggttt cggcgatcac ctggttcacc gacacccccc
    11221 tcctccggca cttcgggctc ccgcggacgc tgcgctggta gcccgccggc gttccgttct
    11281 ggtcgcggcc ggtgtctcaa ccaccagcag acggctcgct ataagcaagg agaactcaat
    11341 gacaaagatc gtcgtgagcg acaacgtcac gctcgacgga gtcatccagg acccggccgg
    11401 tgacgagggc ttcagggtcg gcggctgggt cggcctgatc aaggaccgcc cagagctggg
    11461 caagcttgcg ctggacgagg cgctgggcgc cgaggccctg ctgctggggc ggcggagcta
    11521 cgagtggctc gccgcgcgct ggccatcccg gagtggcgag cttgcggaca ggttgaacag
    11581 cctgcccaag tacgtcgtgt cctcgaccct cgatgatccc gactggaaca actcaacggt
    11641 gctgaagggc aacgtaattg acgagatctc gaagctgaag cagaagctgg acggggacat
    11701 cgtcgtcccg gccagcttcc agctcgtgcg cacgctgatc gagcacgacc tcgtcgatga
    11761 gctgcggctg aagctcttcc cggtcgtgct cggcgccggc gagcgcctct tcggcgagac
    11821 cagcgacaag aaacccatgc gcctcatcaa catccagacc gtcgagggcg acgtcgcgtt
    11881 cctcacctac gaactcgtcc ggaaggccta gcgggcatcg gacaacgcct tcgtttcgaa
    11941 cggccctgat gccgcatcga ggtcgccctt tgagccgact cggtgtgaga agcgcagcgt
    12001 cagctccggg cctgcgcgct gggcgttaag gtctcaacgc cgagcgtggg cagcacgcgc
    12061 tcgagccagt tcgggatcca ccagttgccc ttgccgagca gctgcatcac tgccgggacg
    12121 agcacgagcc gcaccaacgt tgcgtcgagg aagatcgccg aggcgagccc gatccccagc
    12181 agcttcagga agacctcctg ggaggcgagg aacgccagga agacgacgac catgatcgcc
    12241 gctgctgccg tgatcacgcg cgcggtctta gccaggccgt cggcgaccgc ccggcgggta
    12301 tcgccgtcct tcaggtactc ctcgcggatg cgtgagatca ggaagacctc gtagtccatc
    12361 gatagcccga aaaggatcgc gaacatcatc accggcatga acggcgctac cgggacctcg
    12421 tgatcgatgc cgatcaggtt gctgagaccg ccgccgttgg ccgcgagcgt cacaacgccg
    12481 tacgccgccc ccaccgacag caggttcatg acggccgcct tgagcgcgat caggggcgag
    12541 tgaaaggcga caagcaagag caggaacgag agcccgacga caccgctgat gaacgagggc
    12601 atgtgacgtg tgacgtagtc gctctggtcc tcgagtgcgg ctgtgacacc tccgatcttc
    12661 acggtgacac ctgaaccggc gagcgcgctg ggaacgacct tgtcgcgaag gcgcttgacg
    12721 agatcggcgg ttgccttgtc ctgcggggag gtggttggga tcagcgtaat gagagcggcg
    12781 ttgcctgccg tgttgataac cgggcgtgtc acaaacgcga ctcccggctg gctgcgaagc
    12841 ctggccgcga aggactcgat ccgcggtttc gccgcggcgc ccggcagggt ccccgcgatg
    12901 acgaccggtc cgttgctccc ggggccgaag ccgtgggtgt tcaggtcata ggcctgccgg
    12961 gtcatcttgc ctggtgggtc gttccccgcg tcggggaagc cgagccgcat cccgagcgcc
    13021 ggggcggcga gcgcgagcag caccgcagtg gccgcgaccg cggcaggcca aggccgacgc
    13081 tggacggcat ggctccaacg cgcggctgag gattcgcggc cactgccggt gccgagcgac
    13141 cggccggcga gcgggatccg cagccggtcg acccgcgggc cgaggaacgc gagtagcgcc
    13201 gggaggaggg tgaccgacgc gatcatcacg accaagaccg cgagcgaagt cgagattgcg
    13261 accccgtaca tgtatttcag gcccgtcagg cagaggccaa gaacggcgat cacaaccgtg
    13321 cagccggcga tgatcacgct acgcccagcg tgggtgaccg cctcgacgac ggcgtcgtgg
    13381 cggtcctttc cgtcgcgcat ggcggatcgg aagcgggtca ggacgagcag cgcgtagtcg
    13441 atccccacgc cgatgccgat caggccggag acggccgtgg tccagtccgg gacgtcgatc
    13501 acgttcgcga gcagcgctat caggcccgcc gaggagatac cgagtccgac gagcgcgatc
    13561 acgagcggga gcccggccgc gactagcgag ccgaacgcga tcaggaggac gatcgcggcg
    13621 ccgacgaagc cgatcccctc cgggctcgcc tggccctcgg cgtcgtgaat tgggtcgccg
    13681 ccgagtctta tctcgacccc gccgccactg ttttcgttcg cggcctcgat cagctgcttg
    13741 ccctggctcg tcttcacctc ccagccggga acggtgagcg gaagcgtggt cgcgccgatc
    13801 gtcccgtcgc gcgacacgcg agtcggcgtg tgcgccgaga cgtgtttgac cttctccacc
    13861 tgggcaaaga aggcgttgag gcgccgcttg gccgccgggc tcgtggcgcc ggcgtggtct
    13921 ctccagacga cggagatctc ttggcccgaa taaccggcga agcgctgctt ggtgatctcg
    13981 ctcgcggcct tggagtccga tcccggcgtg ttgtagtcgg cgttgtaggc gcccttcgcg
    14041 gcgctgcccg ctccaatgat caggatcgcc gcgccgatcc aggccagcac gacgcgcccg
    14101 cggtggcgat aggcgatgtc gccgagcact gccaggcccc gcgtcacggc cgcatcccgt
    14161 cgctcttgtg actgagtgtc cgcacttctt ccccgtcagt gaagacgagt cggcccgggg
    14221 gcacggcttc gaacagctac gcgcggcgac gcagcgagag cgccggagtt catctcgccg
    14281 accccagtag cgatccgaag tcgccgactg gcccgaggtg cgtcagcttg tcggggttga
    14341 cgatcgcgct gatgcttgtg atctgaccgc cggcgatctc gagcgccagc acgccgatca
    14401 gccgctgctg tgcgtcgaca aggagcgctc ctgggccgcc gttgacctcg acggggcgga
    14461 acgacacgcc cggaaggagg gcgcccaggc ggaaccagtt gatgagcgtg cgcgcgacac
    14521 ggttgcggcc gcggagcgtg cgcgccaagg cgggcacctt gccgccgccg tcgccggtca
    14581 gctccacgtc gtgagcgagc agcgcctcga gcccggcgag atcgccttgc tcggcggcct
    14641 gaaagaaccg tcgtgccagc tcgtcgcgct gctcgcgcgt ggtttggaag cgcgggcggc
    14701 gctgctcgac gtggcgtctg gcgcgggtgg cgagctggcg cacgttgtcc tcgctcttgc
    14761 cgacgatcgg cgcgatctcc ggatacccgt agtcgaacac gtcgtgaagc agcagcacgg
    14821 ctcgctgctc aggcgagagg ctctcca
//
LOCUS       DASVXS010000166        12639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_964774, whole genome shotgun sequence.
ACCESSION   DASVXS010000166 DASVXS010000000
VERSION     DASVXS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964774"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF752_09295"
     CDS             complement(<1..262)
                     /locus_tag="VF752_09295"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEX8855785.1"
                     /translation="MAVRVLRGVAASPGVAAGAARVLGPPVDNGGAPVPLDRRAGELQ
                     RAESALNAAATQLEALAARLRAEGREADAEIVETGVLMAADPG"
     gene            complement(262..915)
                     /gene="dhaL"
                     /locus_tag="VF752_09300"
     CDS             complement(262..915)
                     /gene="dhaL"
                     /locus_tag="VF752_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEX8855786.1"
                     /translation="MTTEAAVKANTVTTWMNEVAAAVGAESAHLTQLDAAIGDGDHGV
                     NMERGFSAVEKALAGQNAELPPGRLLIIAGKTLVSTVGGASGPLWGAALRRAGRSLGD
                     ADSFDGAQLVAALQAAVDGVVELGAAAPGDKTMIDALVPAVDALRNGVDAGEPLEQAL
                     TAAADAADEGARATVPMQARKGRASYLGERSIGHQDPGATSSALIVRALERAVAQAG"
     gene            complement(922..1944)
                     /gene="dhaK"
                     /locus_tag="VF752_09305"
     CDS             complement(922..1944)
                     /gene="dhaK"
                     /locus_tag="VF752_09305"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX8855787.1"
                     /translation="MAVVPRTMKKLLNDPSAVVRESLAGLAAAHPDLVRYDAEAQFVA
                     RADAPVQGKVALVSGGGSGHEPLHGGFVGKGMLDAACPGEVFTSPVPDQILAATKAVD
                     GGAGVIHIVKNYTGDVMNFKLAAEDAADDGIDVESVLIDDDVAVQDSLYTAGRRGVGA
                     TVLAEKIAGAAAERGDGAQSVAAVARRVNERARSFGIALSSCIPPASGKPIFDLPEGQ
                     MEVGIGIHGEPGRRREPLGSAHEIAELLVEPVVTDRPPADGAEVLVFLNGMGGTPQLE
                     LYLLYGEVDALLRERDLVPSRRLVGSYITSLEMAGASLTLLELDDELKELWDAPVHTP
                     ALRWGE"
     gene            complement(1954..2751)
                     /locus_tag="VF752_09310"
     CDS             complement(1954..2751)
                     /locus_tag="VF752_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX8855788.1"
                     /translation="MEEYSLPQRALAEVLGTALLVLVGPGSVVAALELSGKVTPAIRG
                     GDLLGISFAFGFIIAAVVYALGKVSGCHINPAVTFGLALRKRFPWREVPAYWGAQVVG
                     AIIGALAIWGVFAHSGIDLGMGQTSFNQSTTSWGAAVFAEGIGTFMLMFAVLGIVDSR
                     SPSELAGLVIGGVVVAIIMIVGPITGASLNPARAFGPELVSAIGGGATHWNQLIPVYI
                     LPGLVGAGLASIVYDFIAHPRIVKQPIEEAVTHPDDGVPARDVAAVK"
     gene            2934..3566
                     /locus_tag="VF752_09315"
     CDS             2934..3566
                     /locus_tag="VF752_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8855789.1"
                     /translation="MSARADVHTFVFADLAGYTALTEAHGDEHAADEAAAFCEAVRDL
                     LAEHRAEEVKAIGDAMLLRAGEPARAAGLAERIVCGYGARHRGLGVRVGLHTGTAVRR
                     GDDWFGSAVNVASRVADAARAGEVLCTEATMQAIGPAVPVRDRGQHTFKNLANPITVY
                     ELVLARRPELPIDPICRMAIDPEQAAERRLRNGVEYFFCSERCASTFDEA"
     gene            3767..4924
                     /locus_tag="VF752_09320"
     CDS             3767..4924
                     /locus_tag="VF752_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8855790.1"
                     /translation="MPTIEGARQPSGAERAQSKEVLVIGGGQAGLAMGYLLSQEGRDF
                     AILEAAAEPAAAWSARWDSLKLFTSARYSALPGLPIPGDPDRYPTRDEVVDYLRGYAR
                     HFDLPVVLDARVRSLSVANGRYVAATDNAVYEADQIVVATGPFQVPFVPALAEGLDPQ
                     VPQLHSSAYRSPNDISRGPVLVVGGGNSGYQIAEELSRTHEVHMSIGSQQKPLPQRLL
                     GRDLFWFLDATGLIRKSVESRIGRKLAGRDTLIGPKPKTRVRRHGLRMHGRAIRASGS
                     RVGFEDGSELDVGAVIWATGFRTDHSWIDALVFDEHGRLAHRRGVTDSPGLYFLGLTW
                     QHTRGSALIGFVEDDARYIAREIEAFRHTAREESQDGVDRPRAARAPAETV"
     gene            4941..6710
                     /locus_tag="VF752_09325"
     CDS             4941..6710
                     /locus_tag="VF752_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX8855791.1"
                     /translation="MSTEADKFPTATAGLPEAQRPELIELADGDRFELRIAPVTKRLG
                     DNVVRMLAYNGSIPGPVLKVREGSEIVVDVVNEGDMEATVHWHGLRLENRYDGTHETQ
                     AAIPVGGSYTARVVFPDSGVYWYHPHIREDYGQEMGLYGNVLVEPTATDYWPPADREV
                     PLTLDDVLIENGGIAPFSRAETTYAAMGRFGNVLLVNGETDLSLQARQGEVLRLYLTN
                     TANTRVFKVALPGARMKLVGGDSGHVEREQLIEEAIIAPSERVVVDVLFDQSGSLTLE
                     HRTPGKTYPLAAIEVEDNGGKSDAAEVFASLRTNADMVAERERIAAYLEAEPDKTLAF
                     IAEMDLAGPEGPVVYACPMHPEVVSEEPGNCPQCGMKLLATSTTFTCPMHPEVVSEEP
                     GHCPKCGMKLLPAQLVAQAGGEHHHGEHGDMHEHHGHHAHGHDHEHEGHAHGTADGIE
                     WEDDMVDVNRLTTPANMRWKLIDRETGDENAAIDWRFRVGDRIKIRLLNEMAGDHPMH
                     HPFHVHGAGRFLILARDGAVDPNLVWKDTVLVRAGETVDILLDVTNVGRWMAHCHIAE
                     HHESGMMFSFEVDPAENGAPESR"
     gene            complement(6813..7823)
                     /locus_tag="VF752_09330"
     CDS             complement(6813..7823)
                     /locus_tag="VF752_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX8855792.1"
                     /translation="MSEQLARQPLRPLEATEPPEARGLRRDEVRMMVATRSDGAIRHA
                     RFNELSGLLEPGDLLVVNNSATLPAALPAARSDGIALELHLSTPAPDQAPNWWVVELR
                     RDSAPFKEGQHGEEIRLAGGGRAVLVAPFARGNRLWLARLDLPVPVEEYLARHGHPIR
                     YGYVPRAWPLEAYQNVYALEPGSAEMASAGRPFTPEVLTQLMARGVLVAPITLHTGVS
                     SPEAHEAPFPERYRVPAETARLIDVVHGWGGRVIAVGTTVVRALESGEGEGWTNLVIT
                     PERGLRDVDGLLTGWHEPESSHLQMLEAVAGPELLARSYELALEHGYLWHEFGDVQLI
                     LP"
     gene            complement(7820..8500)
                     /locus_tag="VF752_09335"
     CDS             complement(7820..8500)
                     /locus_tag="VF752_09335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8855793.1"
                     /translation="MPTAIVTGASRGLGLALSRALVKRGWRLVVDARGGEALDREWGN
                     TLGVVALAGDVADPEHREALVAAAAGPIDLLVNNASLLGPSPQPPLASYPLDELRKVY
                     EVNVAAPLALVQEALPLLYQGSGRVLNITSDASVEPYEGWGGYGSSKAALDQLSAILA
                     AEHPELRVYAADPGDMRTQMHQDAFPGEDISDRPPPEDSVPGLLALIEGDLPSGRYSV
                     RELAKVSA"
     gene            complement(8551..9192)
                     /locus_tag="VF752_09340"
     CDS             complement(8551..9192)
                     /locus_tag="VF752_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855794.1"
                     /translation="MADEIRVLIVDDHAVVREGLRTFLELQDDMQVVGEAGDGDDAVA
                     LAKRFEPDVVLMDLVMPGVDGIEAMRRLRRQVPSARAIVLTSFIDEDRILPAIRAGAA
                     GYLIKNVEPQELARAVRAAADGETLIDPVVASRLVEVLADERDAPRPEQLTPREQEVL
                     QLIGRGYSNKRIALELGVAEKTVKNHVSHVLSKLGVADRTQAALYAARVGRES"
     gene            complement(9185..10324)
                     /locus_tag="VF752_09345"
     CDS             complement(9185..10324)
                     /locus_tag="VF752_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX8855795.1"
                     /translation="MQERASLSPTLKAVSDAVLAVAAESSVEDVLQRLVHSARELADA
                     RYSALGIPDGEGGFSQFITSGMSDELIVSLGPLPRQHGMLGAMLGSEQSFRTPDIHDD
                     PRFRGWWPRNHPDMRSFLGVPIVAREGVIAAFYLTEKIGAPEFSDEDQELIELLAAHA
                     SIAITNARLYEQSRQLSILSERNRLALELHDAVSQKLFSLVLTAEAAGTLFERDPEAA
                     REQVTKLRTLTQEALEELRSLIFELRPPDLDRDGLCGALRKHVEVVRRLWAGTIALEL
                     DAGVALDPESDVEILRIAQEALQNAVRHAHARHVNVSLRRMDGRVVLQVSDDGVGFDP
                     TAAELRARRLGLTSMEERAQRLGGTLAIRSAQNEGTTVRMEVDHG"
     gene            complement(10530..11006)
                     /locus_tag="VF752_09350"
     CDS             complement(10530..11006)
                     /locus_tag="VF752_09350"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX8855796.1"
                     /translation="MKRGLMLVALLAAVAVVAILPLTSGAQTRGERKALFGAMTGAKE
                     VSATGQRRAGDLNGRGSFTAIIDGTQICYGITVTNIASPVAAHIHRGGPNVAGPIVLP
                     LQQPASGDPGASSECKAIPSALARAILKNPSRYYVNVHTSDFPGGAVRSQLFGQSR"
     gene            11110..>12639
                     /gene="hutH"
                     /locus_tag="VF752_09355"
     CDS             11110..>12639
                     /gene="hutH"
                     /locus_tag="VF752_09355"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX8855797.1"
                     /translation="MVGSASGVIQLSGTGLAPDDVIAVARAGARVELSDEARRTMDES
                     AAVVARIAESDEPAYGVSTGFGALAIVAIPDERREELQRALVRSHAAGMGPPVEAEVV
                     RAMMLLRARTLAMGYSGARPLVADTMLALLNAGVTPVVPEHGSLGASGDLAPLAHCGL
                     VLIGEGEAHTAGGARVSGADALQATGVEPITLIAKEGLALINGTDGMLGMLLLGLHDL
                     ALLLRVADVTAAMSVEALLGTDRAFADDLVALRPQPGQQLSAANLRRLLAGSLIVASH
                     RYGDDRVQDAYSLRCAPQVAGAARDAVAHAARVADAELLSAIDNPMVLPDGRVESCGN
                     FHGAPLAHALDFLAIASADVGSIAERRTDRLLDFARSYGLPPFLTEDAGVNSGLMIGQ
                     YTQAAMVAENRRLATPASVDSVPTSAMQEDHVSMGWGAARKLRRSVRNLGRILAVELV
                     CAAAGLDFRAPLEPGPGTTAALTAVRSRVAGPGPDRWVAPDLAAAEELIWSGELIAAV
                     EA"
BASE COUNT         1961 a         4454 c         4343 g         1881 t
ORIGIN      
        1 gcccgggatc ggccgccatc aggacacccg tttcgacgat ctcggcatcc gcttcgcggc
       61 cctcggcgcg aagccgggcg gcgagcgcct cgagttgggt cgcggccgcg ttgagcgccg
      121 actcggcacg ttggagctcg cccgcgcgcc ggtcgagcgg gaccggcgca ccgccgttat
      181 cgacgggcgg cccaagtacc cgggcggcac ccgcggcaac ccccggcgag gccgccacgc
      241 cccgtaggac gcggacggcc atcagcccgc ctgcgcgacc gcgcgctcga gggcgcgcac
      301 gatcagggcg ctcgacgtcg cgccggggtc ttggtgcccg atcgagcgtt cgcccagata
      361 cgaggcgcgg cccttgcgtg cctgcatcgg cacggtggct cgcgcgcctt cgtcagccgc
      421 gtcggcggcc gcggtgaggg cctgctcgag gggctcgccg gcatccaccc cgttgcgcag
      481 cgcgtccacc gcgggcacaa gcgcgtcgat catcgtcttg tcgcccggcg ccgcggcgcc
      541 gagctcgacc acgccgtcga ccgctgcctg aagggccgcg accagctgcg cgccgtcgaa
      601 agaatccgca tcgccgaggc tgcgcccggc gcggcgaagc gccgcgcccc acagaggccc
      661 gcttgcgccg ccgaccgtcg agacgagcgt cttgcccgcg atgatgagga gccggccggg
      721 cggaagctcc gcattctgcc cggccagcgc cttctcgacc gcggagaagc cgcgctccat
      781 gttcacgccg tggtcaccgt caccgatcgc ggcgtcgagc tgggtcagat gggcgctctc
      841 cgcgccgacc gcagccgcga cctcgttcat ccaggtggtg acggtgttgg ctttcaccgc
      901 ggcttccgtc gtcactgcgc atcactcccc ccagcgcaga gcgggcgtgt gaaccggagc
      961 atcccacaac tccttcagct catcgtcgag ctcgagaagg gtgagcgagg cgcccgccat
     1021 ctcgagcgag gtgatgtagc tgccgacgag ccgccggctc ggcacgaggt cgcgctcgcg
     1081 caggagcgca tcgacctctc cgtagagcag atacagctcg agctgcggcg tgccgcccat
     1141 gccgttcagg aacacgagca cctcggcgcc gtccgcaggc ggccgatcgg tgaccaccgg
     1201 ctccacgagc aactcggcga tctcgtgcgc gctgccgagc ggctcgcgcc gccggcccgg
     1261 ctcgccgtgg atccctatgc ccacctccat ctgcccctcg ggcaggtcga agatcggctt
     1321 gcccgaggcc ggcgggatgc acgacgagag cgcgatgccg aacgagcgcg cgcgctcgtt
     1381 cacccgtcgg gcaaccgcgg cgacggactg agcgccgtca ccgcgctccg ccgccgcgcc
     1441 cgcgatcttc tcggcgagca cggtcgcgcc cacgccacgg cgcccggccg tgtagagcga
     1501 gtcctgcacc gccacgtcgt cgtcgatcag cacggactcg acgtcgatgc cgtcgtcggc
     1561 cgcatcctcg gcggcgagct tgaagttcat cacgtcgccc gtgtagttct tgacgatgtg
     1621 aatcactccg gcgccgccgt cgaccgcctt ggtcgcggcg aggatctggt ccggcaccgg
     1681 cgacgtgaac acctcaccgg ggcacgcggc gtcgagcatt cccttgccga cgaagccgcc
     1741 gtgcagcggc tcatgacccg agccgcctcc ggagaccagc gccaccttgc cctgcactgg
     1801 tgcatcggca cgcgccacga actgagcctc ggcgtcgtac cggaccagat cggggtgagc
     1861 ggccgccagg cctgccagcg actccctcac gaccgcggac gggtcgttca ggagcttctt
     1921 catcgttcgc ggtacgactg ccatcgcaac ctcctacttg acggcggcga cgtcgcgcgc
     1981 gggcacgccg tcgtcgggat gggtcacggc ctcttcgatc ggctgcttca cgatgcgcgg
     2041 atgcgcgatg aagtcgtaga cgatcgacgc cagcccagcg ccgacgaggc cgggcaggat
     2101 gtagaccgga atgagctggt tccagtgagt agcgccgccg ccgatggcgg agacgagctc
     2161 tgggccgaac gcacgcgccg ggttgagcga cgcgccggtg atcggaccga cgatcatgat
     2221 gatcgcgacc accacgccgc cgatcacgag gccggcgagc tcactcggtg aacgcgagtc
     2281 cacgatgcca agtacagcga acatcagcat gaaggtgcca atgccctccg cgaagacggc
     2341 cgctccccag ctcgtggtgg actgattgaa cgacgtctgg cccatgccca ggtcgatgcc
     2401 ggagtgggcg aacacgcccc aaatggccag cgcgccgatg atcgcgccca cgacctgggc
     2461 gccccagtaa gccgggacct cgcgccacgg gaaccgcttc cgcagagcca ggccgaaggt
     2521 cacggcgggg ttgatgtgac atcccgagac cttgccgagg gcgtacacca ccgcagcgat
     2581 gatgaacccg aaggcgaatg agatcccgag cagatctccg cccctgatcg ccggcgtgac
     2641 cttgcccgag agctcgagcg ccgcgaccac agatccgggc cccacgagca cgagtagcgc
     2701 ggtgccgagt acctcggcca gcgctcgctg aggaagcgag tattcctcca ttgcaacctc
     2761 tcctctcgcc tctgacttcc cgatcagtat tcgataatgt cgaatactgt tccgggcgcg
     2821 caccgtactc cctcgtaacg cccgtgtcaa gcagagacgt ctcacacgcg cggctccatg
     2881 gtccgcgacc cggtcactga cgcaccgggg tcgctctgcc atcctcgagc gcgatgagcg
     2941 cccgcgccga tgtccatacc ttcgtcttcg ccgacctggc cggttacacc gcactgacgg
     3001 aagcgcacgg cgacgagcac gccgcggacg aggcggccgc gttctgtgag gccgttcgtg
     3061 acctgttggc cgagcatcgc gccgaggaag tcaaggcaat cggagatgcg atgctgctgc
     3121 gggcgggcga acctgcaagg gccgccggcc tcgccgagcg catcgtgtgc ggatacggag
     3181 ctcgccatcg tgggctcggg gttcgagtcg gcctacacac gggcaccgca gttcgccgcg
     3241 gcgacgactg gttcggcagt gccgtgaacg ttgcctcccg cgtcgcggac gccgcccgag
     3301 ccggcgaagt gctctgcaca gaggcaacca tgcaggcgat cggtcccgcc gtccccgtgc
     3361 gcgaccgcgg tcagcacacg ttcaagaacc tggccaaccc gataaccgtg tacgagctgg
     3421 tgctggctcg acggccggaa cttccgatcg atcccatctg ccgcatggcg atcgatcccg
     3481 agcaggccgc ggagcgtcgg ctgcgcaacg gggtcgagta cttcttctgc tctgagcgct
     3541 gcgcgtcgac cttcgacgaa gcctgatcaa cgccacctcg ccgccgcaca cttgcccttc
     3601 tgtggcaggt gtatggcccg aagtggaagg cggctccttc cggggctaac ctcgccgtag
     3661 acggtcaggc tgcggactcg caatcggttt cttgcccccg tcccgagggc ggtccgacgc
     3721 agcttggccc tgaagaagat ctcgcgttcg taggagggcg ctccacatgc ccacgatcga
     3781 aggcgctcga cagccgagcg gtgccgagcg cgcgcagagt aaggaggtgc tcgtcatcgg
     3841 cggcggccag gccggcctcg ccatgggcta cctcctgtcc caggaagggc gcgacttcgc
     3901 gatcctcgag gcggctgcgg agccggccgc cgcgtggagt gcgcgctggg actcgctgaa
     3961 gctgttcacc tcggcgcggt attcggcgct tcccgggctc cccataccgg gcgaccctga
     4021 ccgctatccg acccgtgacg aggtcgtcga ttacctccgc ggctacgcac ggcacttcga
     4081 tctgccggtc gtgctcgacg ctcgggtccg gtccctctcg gtggcgaacg gacggtatgt
     4141 cgcagccacc gacaacgcgg tctacgaggc ggatcagatc gtggtcgcga cagggccatt
     4201 ccaagtcccc ttcgtgcccg cgctcgccga gggcctcgac ccgcaggtcc cgcaactgca
     4261 cagcagcgcc taccgctccc cgaacgacat atcgcgcggg ccggtgctgg tagtcggcgg
     4321 gggtaacagc ggctaccaga tcgccgagga gctttcgcgt acccacgagg tccacatgtc
     4381 gatcgggtcg cagcagaagc cgctccccca gaggctgctg ggccgcgact tgttctggtt
     4441 cctcgacgcc acaggactga tccgcaagtc ggtcgagtcg aggatcggcc gtaagctcgc
     4501 cgggcgcgac acattgatcg gcccgaagcc gaagacccgc gtgcggcgcc acggtttgcg
     4561 gatgcacggg cgagcaatcc gtgcgagtgg atccagggtc ggcttcgagg acgggtcaga
     4621 gctcgacgtg ggcgcggtga tctgggccac aggcttccgg accgaccatt cctggatcga
     4681 cgcgctggtg ttcgacgagc acggtcgtct cgcgcatcgg cgcggcgtca cggactcgcc
     4741 cggcctgtac ttcctcggcc tcacctggca gcacacgagg ggctccgccc tgatcgggtt
     4801 cgtcgaggac gacgcccgct acatcgcgcg cgagatcgag gcgttccggc acacggctcg
     4861 ggaagaaagc caagacgggg tcgatcgacc gcgagccgcc cgcgcgcccg ccgaaacagt
     4921 ctgagcaact ggagattgag atgagcacag aggcagacaa gttcccaacg gcgaccgccg
     4981 gccttcctga ggcgcagcgt cccgagctga tcgagctcgc cgacggcgat cgcttcgagc
     5041 tgcgcatcgc gccggtgacc aaacgactcg gcgacaacgt ggtgcggatg ctcgcgtaca
     5101 acggctcgat cccgggtccg gttctgaagg tccgcgaggg ctcggagatc gtggtcgacg
     5161 tggtcaacga gggcgacatg gaggcgaccg tccattggca cgggctgcgc ctcgaaaacc
     5221 ggtacgacgg aacgcacgag acgcaggcgg ccatcccggt cggcggcagc tacacggcgc
     5281 gagtcgtatt ccccgactcc ggcgtctact ggtaccaccc gcacatccgc gaggactacg
     5341 ggcaggagat gggcctctat ggcaacgtcc tcgtcgagcc gaccgctacg gactactggc
     5401 cgccggccga tcgtgaggtc ccgctcacgc tcgatgacgt gcttatcgag aacggcggga
     5461 tcgcgccgtt cagccgggcc gagacgacct atgcggccat gggtcgcttc gggaacgtgt
     5521 tgctcgtcaa cggcgagacc gacctctcgc tacaggcgcg ccagggcgag gtactccgcc
     5581 tttacctgac caacaccgcc aacacgcgcg tgttcaaggt cgcgcttccc ggggcgcgca
     5641 tgaagctggt cggcggggac agcggacacg tcgagcgcga gcagctgatc gaggaggcga
     5701 tcatcgcgcc ctcggagcgc gtggtggtcg acgtcttgtt cgatcaaagc gggagcctga
     5761 cgctcgagca ccggacgccc ggcaagacgt acccgctcgc cgcgattgag gtcgaggaca
     5821 acgggggaaa gtcggacgcc gcagaggtgt tcgcgagcct gcgcacgaac gccgacatgg
     5881 tcgctgagcg cgaacggata gccgcctatc tcgaagcgga gcccgacaag acgcttgcct
     5941 tcattgcaga gatggaccta gcgggtcccg aggggccggt cgtctacgcc tgtccgatgc
     6001 atccggaggt ggtcagcgaa gagccgggca actgtcccca gtgcgggatg aagcttctcg
     6061 ccacgtccac gacgttcacg tgcccgatgc accctgaggt cgtcagtgag gagccgggac
     6121 actgcccgaa gtgcggcatg aagctgctgc cggcgcagct ggtcgcgcaa gccggcggcg
     6181 aacaccacca cggcgagcac ggcgacatgc acgagcacca tgggcaccac gcgcacggcc
     6241 atgaccatga gcatgagggc cacgcgcacg gaacggcgga cggcatcgag tgggaagacg
     6301 acatggtcga cgtgaatcgg ctgaccacgc cggccaacat gcgctggaag ctgatcgacc
     6361 gcgagacagg cgatgagaac gccgccatcg attggcgctt ccgcgtcggc gaccggatca
     6421 agatccggct gctcaacgag atggccgggg accatccgat gcatcacccc ttccacgttc
     6481 atggagcggg acgcttcctg atcctcgccc gtgacggtgc agtcgatccc aacctcgtct
     6541 ggaaggacac ggtgctggtc cgagctggcg agacggtgga catcttgctc gacgtgacga
     6601 acgtcggccg ctggatggcg cactgccaca tcgccgagca ccacgagagc ggaatgatgt
     6661 tcagcttcga ggtcgatccc gccgaaaacg gcgcaccgga gtcgcgatga agatcgccta
     6721 cgtcctctac cccgacttca cggcgctcga tttggtgggc ccgtacgagt gatcagccgc
     6781 tggccctagg ggacaccgat cgacgctcgg tgctacggga ggatcagctg gacgtcgccg
     6841 aactcgtgcc agaggtagcc gtgctcgagc gctagctcgt acgagcgcgc aagcagctcg
     6901 gggccggcga cggcctcgag catctgcagg tgcgacgact ccggctcgtg ccagccggtg
     6961 agaagaccgt cgacgtcgcg aagccctcgc tccggcgtga tgaccaggtt cgtccagccc
     7021 tcgccctccc ccgactcgag cgcacgtacc acggtggtgc cgacggcgat cacgcgcccg
     7081 ccccagccgt gtaccacatc gattaggcgc gcggtctcgg ccggcacgcg gtagcgctcg
     7141 gggaaaggcg cctcgtgcgc ctccggcgac gacacgcccg tgtgcagcgt gatcggcgca
     7201 accagcacgc cgcgcgccat cagctgggta agaacctcgg gcgtgaacgg ccgtccggcg
     7261 ctcgccatct cggcgctgcc gggctcgagt gcgtacacgt tctggtaggc ctcgagcggc
     7321 cacgcgcgcg gcacgtagcc gtagcggatc gggtgcccgt ggcgcgcgag gtattcctcg
     7381 accggaacgg gcaagtcgag gcgcgccagc cagagtcggt tgccgcgcgc gaacggtgcg
     7441 accaagacgg cgcggccacc accggcgagg cggatctcct caccgtgctg cccctccttg
     7501 aacggagccg agtcgcgccg gagctccacc acccaccagt tcggcgcctg gtccggcgcc
     7561 ggtgttgaga ggtggagctc gagcgcgatg ccgtcgctcc gcgcggctgg gagtgcggcg
     7621 gggagggtgg ccgagttgtt caccacgagc aggtcgccgg gctcgagcag gccagacagc
     7681 tcgttgaagc gcgcatgccg gatcgcgcca tcggaacggg tcgcgaccat catgcgcacc
     7741 tcgtcgcggc gcaatccacg cgcctcgggc ggctcggttg cctcgagcgg acgcagcggc
     7801 tggcgggcga gctgctcgct caagcgctca ccttcgccag ctcgcgcacg ctgtagcggc
     7861 cgctgggcaa gtcaccctcg atcagcgcca ggaggcccgg aacgctgtcc tcgggcggcg
     7921 gacggtcgga gatatcctcg ccggggaacg cgtcctggtg catctgcgtg cgcatgtcgc
     7981 ccgggtcagc ggcgtacacg cgcaactccg gatgctcggc ggcgaggatg gcgctcagct
     8041 ggtcgagtgc cgccttcgac gagccgtatc cgccccagcc ctcgtagggc tcgacggagg
     8101 cgtcggaagt gatgttgagc acgcgcccgc tcccctggta gagcagcggg agtgcctcct
     8161 gtacgagcgc gagcggcgcc gccacgttca cctcgtacac cttccgcagc tcgtcgagcg
     8221 ggtagctcgc gagcggcggc tgcggactcg ggccgaggag gctggcgttg ttcaccagca
     8281 ggtcgatcgg gccggccgca gcggccacga gcgcttcgcg gtgttcggga tcggcgacgt
     8341 cgccggccag ggcgaccacg ccgagcgtgt tgccccactc gcgatcgagc gcctccccgc
     8401 cacgggcgtc gaccacgagc cgccagccgc gcttcacgag agcgcgcgat agcgcgaggc
     8461 cgagccctct ggatgctcct gtgactattg ctgttggcat gcccggcacg ctaggtgcgg
     8521 gcgggtcccc gttaccaggg gcaattggtc ctaggactcg cggcccacgc gcgccgcgta
     8581 gagcgctgcc tgggtgcgat cggccacgcc gagcttcgac agcacgtggc tcacgtggtt
     8641 cttcacggtc ttctcggcca cacccagctc gagcgcgatg cgcttgttcg agtagccgcg
     8701 cccgatcagc tggagcacct cctgctcgcg cggcgtgagc tgctcgggcc gcggggcgtc
     8761 gcgctcgtcg gcgagcacct ccacgagccg cgaggccacg accgggtcga tcaaggtctc
     8821 gccgtcggcc gcggcgcgga cggcgcgcgc gagctcctgc ggctcgacgt tcttgatcag
     8881 gtagcccgcg gcaccggcgc ggatcgccgg caggattcgg tcctcgtcga tgaagctcgt
     8941 cagcacgatc gcgcgtgccg acggcacctg acgccggagg cgacgcatcg cctcgatgcc
     9001 gtccacgccc ggcatcacga ggtccatgag caccacgtcc ggctcgaagc gctttgccag
     9061 cgccacggcg tcgtcgccgt cccccgcctc gcccaccacc tgcatgtcgt cctggagctc
     9121 gaggaacgtg cgcagcccct cgcgcaccac agcgtggtcg tccacgatca gcacgcggat
     9181 ctcgtcagcc atggtcgacc tccatgcgca ccgtggtccc ctcgttctgg gccgagcgaa
     9241 tggcgagtgt cccgccgagc cgctgcgcgc gctcctccat cgaggtgagg ccgagccggc
     9301 gagcgcgaag ctcggcggcc gtggggtcga agcccacgcc gtcgtccgaa acctggagca
     9361 ccacacgacc atccatgcga cgcagcgaca cgtttacgtg gcgcgcatga gcgtggcgca
     9421 ccgcgttttg aagggcctcc tgtgcgatgc gcaggatttc gacgtcgctc tcggggtcga
     9481 gcgcgactcc ggcgtccagc tcgagcgcga tcgtgcccgc ccacagccgc cgcaccacct
     9541 ccacgtgctt gcgcagcgca ccgcagagcc cgtcgcggtc gaggtccggc gggcgcagct
     9601 cgaagatgag cgagcggagc tcctcgagcg cctcctgcgt gagagtgcgc agcttcgtga
     9661 cctgctcgcg cgccgcctcg gggtcgcgct cgaagagcgt gcccgcggcc tccgcggtca
     9721 ggacgaggct gaacagcttc tggctcaccg cgtcgtgcag ctcgagcgcg agcctgttgc
     9781 gctcggacag gatcgacagc tggcggctct gctcgtagag gcgcgcgttg gtgatcgcga
     9841 tcgacgcgtg cgcggccagc agctcgatca gctcctggtc ctcgtcggag aactccggcg
     9901 cgccgatctt ctcggtgagg tagaacgcgg cgatcactcc ttcgcgcgcg acgatcggca
     9961 cgcccagaaa cgagcgcatg tcggggtggt ttcgcggcca ccagccgcgg aagcgcgggt
    10021 cgtcgtggat gtcgggcgtg cggaacgact gctccgaccc gagcatcgcg ccgagcatcc
    10081 cgtgctggcg cggcagcggc ccgagcgaca cgatcagctc gtcgctcatt cccgaggtga
    10141 tgaactggct gaagccgccc tcgccgtcgg ggatgccgag cgccgagtac cgcgcgtcgg
    10201 cgagctcgcg cgcgctgtgt acgaggcgct ggagcacgtc ctcgaccgaa gactcggcag
    10261 cgaccgccaa caccgcgtcg ctaacggctt tgagcgtggg actgaggctt gcgcgctcct
    10321 gcataccggt cccacgatac gcccggccga ctgaggcgct gtgaacagca gcaattaggg
    10381 gtgaggcact ggtcgagacg tgggtgacct tggttgcgca tatcgcaact aaagtcaccc
    10441 acgcccagag cggcgaggcg cgcgaccgtc ccgcctagcc gacgatcagg tggatcgggg
    10501 ccgcccgccc agcgagcggc ccctgatccc taacgcgact ggccgaacag ctggctgcga
    10561 acggcaccgc ccgggaagtc ggacgtgtgc acgttcacgt agtacctgct cgggttcttc
    10621 aggatcgccc ttgcgagcgc tgacggaatc gccttgcact cgctcgacgc acccgggtcg
    10681 ccgctggccg gctgctggag cggcagcacg atcgggcctg ccacgttcgg gcctccgcga
    10741 tggatgtgag cagccacggg cgacgcgatg ttcgtgacgg tgatgccgta gcagatctga
    10801 gtcccgtcga tgatggccgt gaagctgcct cggccgttga gatctcctgc acgcctctga
    10861 ccggttgcgc ttacttcctt cgccccggtc atcgctccga agagcgcttt cctctcgcct
    10921 ctcgtctgag ctccggacgt gagcggcaag attgcgacta ccgcaacggc ggccagcagc
    10981 gcgacaagca tgaggccacg cttcatctga aacacctccc cttggactgg cgggccagcc
    11041 tttctcccaa tagacgctgc gtgttcccgg atctatcccc gggcatgggc gagatatcgt
    11101 ggctcgccga tggtgggcag cgcttcgggc gtaatccagc tgagcgggac cggcttggct
    11161 ccagacgacg tgatcgccgt cgcgcgcgcg ggcgcgcgcg tggagttgag cgacgaggcg
    11221 cgccgcacga tggatgagag cgcggcggtg gtcgcgcgta tcgcggagtc cgacgagccc
    11281 gcctatggcg tctcgaccgg ttttggcgcg ctggcgatcg tcgcgatccc cgacgagcgc
    11341 cgcgaagagc tccagcgcgc gctcgtgcgc tcgcacgccg cgggcatggg gccaccggtc
    11401 gaggccgagg tggtgcgcgc gatgatgctg ctgcgggcgc gcacgctcgc gatgggctac
    11461 tcgggtgcgc gcccgctggt tgccgacacg atgctggcgt tgctcaacgc cggcgtcacg
    11521 ccggtggtac ccgagcacgg ctcactcggc gccagcggcg acctcgctcc gctcgcgcac
    11581 tgcggcctcg tactgattgg cgaaggcgag gctcacacgg ccggcggcgc gcgggtctca
    11641 ggcgccgacg cgctccaggc gaccggcgtc gagccgatca cgctgatcgc caaggagggc
    11701 ctcgcgctca tcaacggcac cgacggaatg ctcggcatgc tgctgctggg gctgcacgat
    11761 ctcgccctgc tcctacgagt ggccgacgtg accgcggcga tgtcggtcga ggccctgctc
    11821 ggcaccgatc gcgcgttcgc cgacgatctc gtggcgctgc gaccccagcc gggccagcag
    11881 ctgagcgccg ccaacctacg gcgtctgctc gccggctcgc tgatcgtcgc gagccaccgc
    11941 tatggcgacg accgcgtgca ggacgcgtac tcgctccgct gcgcgccgca ggtggcgggc
    12001 gccgcacgcg acgccgtggc ccacgccgcg cgagtggccg acgcggagct tctgtcggcg
    12061 atcgacaacc caatggtgct ccccgacgga cgcgtggagt catgcggcaa cttccacggc
    12121 gcgccgctcg cgcacgcgct cgacttcctg gcgatcgcga gcgcggacgt cggctcgatc
    12181 gccgagcggc gcaccgaccg gctgctcgac ttcgctcgct cctacggcct gccgccgttc
    12241 ctgacggaag acgccggcgt gaactctggc ctcatgatcg gccagtacac gcaggccgcg
    12301 atggtggctg agaaccggcg cctggcgacg cccgcgagcg tggactccgt gcccacgagc
    12361 gcgatgcagg aggaccacgt gtcgatgggc tggggcgccg cgcgcaagct gcggcgctcg
    12421 gtgcggaatc tcggacgcat actcgccgtc gagctcgtgt gcgcggcggc aggcctcgac
    12481 ttccgggcgc cgctcgagcc cgggccggga acgactgcgg ctctcactgc ggtgcgctcg
    12541 cgcgtcgcgg ggcccgggcc cgaccgctgg gtcgcccccg acctcgccgc cgctgaggag
    12601 ctcatctggt cgggcgagct gatcgctgcc gtcgaagcc
//
LOCUS       DASVXS010000167        11569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_539616, whole genome shotgun sequence.
ACCESSION   DASVXS010000167 DASVXS010000000
VERSION     DASVXS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539616"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VF752_09360"
     CDS             complement(<1..751)
                     /locus_tag="VF752_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8855798.1"
                     /translation="MTREQTAAPPVAQGASAASVDIRLSGLRKTFAGGVTAVDDVSLE
                     IREGEFFTMLGPSGSGKTTCLRLIAGFEQPDSGGIELAGQDVTKVPPEERNVNTVFQD
                     YALFPHMSVGENVEYGLRVKGVGKAERRERAREALEMVRLEDYGKRRPGQLSGGQRQR
                     VALARALVNRPRVLLLDEPLGALDLKLRQQLQVELKRIQSDVGITFVYVTHDQDEALT
                     MSDRIAVMDDGRVLQVGAPNEVYDEPDSSFVA"
     gene            complement(856..1707)
                     /locus_tag="VF752_09365"
     CDS             complement(856..1707)
                     /locus_tag="VF752_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX8855799.1"
                     /translation="MTTLPCTFVDVFTDVRFEGNGLAVVHDADGLDESLMLAFARETR
                     LSETTFVQSATNNGADYRNRIFMMSGEVPFAGHPSLGTAVAVARARGEETARYVQQTH
                     AGLQPIEVRKDGDAWIASMLQEPPEFGEEPPPDVVLAAVSLEPGEAHPELPAQVVATG
                     VPQLLLPVNGLDALERIEPRYEFVNALLQPIGALTLYVFAIADDGEVRSRAFTATAEM
                     GEDPATGSAAGPVCAYLNARLGWESVAIRQGVEMGRPSRLSARVVEDRVEVAGGVVPV
                     IDGTLTL"
     gene            1798..1989
                     /locus_tag="VF752_09370"
     CDS             1798..1989
                     /locus_tag="VF752_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX8855800.1"
                     /translation="MGFLDKLTGRTKKAAGDLTDDPSLRREGRQEERKGEAKDELSNA
                     QERADAKADEVADLERKTN"
     gene            complement(2160..2789)
                     /locus_tag="VF752_09375"
     CDS             complement(2160..2789)
                     /locus_tag="VF752_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855801.1"
                     /translation="MAARTRCLGVDDHPVVRQGLDLLFGELDDLELVGTVTTGEEALA
                     AVKRLKPQVVLVDVRLPGIDGISTLKRIHEEAPEVALVVFSAYGDKRLLSDAIAAGAR
                     GYVMKGSPPEDLLRAIRTVVGGKAFVDPSLSPALLMTNSAQAEQPLSEREREILQLLA
                     EGYHTEEIARRIGLSVETVKSDTKRVIAKLQADTRTHAVAIALRQSLID"
     gene            2927..3370
                     /locus_tag="VF752_09380"
     CDS             2927..3370
                     /locus_tag="VF752_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8855802.1"
                     /translation="MFGSIVVGTDGSDTAKEAVRQATVLAKSTGDELLVVSAYEPVPE
                     GRLRSERRDTPEDLQWTINPREDVETTLEDASDSIGESGVKVQTFAREGDPADAILDV
                     AEEQNAGLIVVGNKGMTGAKRFLLGSVPNKVSHHAPCSVLIIRTT"
     gene            complement(3367..4617)
                     /locus_tag="VF752_09385"
     CDS             complement(3367..4617)
                     /locus_tag="VF752_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8855803.1"
                     /translation="MIVKYRSGVAKSARAAVQRQTGASYAGKLPGGPRTLRIRDSKTV
                     GETVQTLRRHPSVEYAVPNYLAHASFIPNDPGLTGQAGGWQALQWDLAGPASVNAPAA
                     WDEAIRAGAPGGRGVVVAVIDTGVAYENFRRFKKSPDLYRQRFVRGFDFVDNDRHPND
                     ENGHGTHVTSTIAEATNNGFGLVGLAYGVKIMPLRVLDSEGAGDAAAISRAIRYAANN
                     GARVINMSLEFDTTVTASQIPEIVSAARYAHRKGVTMVAAAGNEADTAVAYPARAGQI
                     ISVGATTQHLCQADYSNAGPGLDISAPGGGNDAPNVDNPSDQANCHPGVDLPPIFQVT
                     FRHDGGIRTFGVRGSEYEGTSMASPHVAAAAALLIATKRLGSRPSPALIQDRLARTAR
                     DLGPPGYDTRYGAGLLDVAAALAP"
     gene            4808..5920
                     /locus_tag="VF752_09390"
     CDS             4808..5920
                     /locus_tag="VF752_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8855804.1"
                     /translation="MKRSCVTGATGFVGAHVARMLAERGHEVRVTYRDETRLTRLSDT
                     DVEPVRADVLDRAAMRRAVRGADVLFHTAGYVASRPADRVFEINALSVRVALEAAAAE
                     GVGRVVVTSSVGALGPADAGELIDEGHLYRGTGLAYADAKHEGEAEAFAAAARLGVEV
                     VVVEPAYVLGVPVDHTQPGETSTRVIGNYLQGRLPAVMEGGASFVDVDDVAAGHLAAA
                     DRGASGERYVLGGHNLAWCELIERVAELSGVRHPIVVLPPEMETVARVQRSLRLPLLI
                     SPEAYALMGADWRCSSRKAKRELDYGVRSLDRTLHATIEWYQELIEAGAFRGRAPSSL
                     SLASAGLRVAERAGLFPFVRAAERWSGRQLLAGTRE"
     gene            5917..6387
                     /gene="tadA"
                     /locus_tag="VF752_09395"
     CDS             5917..6387
                     /gene="tadA"
                     /locus_tag="VF752_09395"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX8855805.1"
                     /translation="MNVESGFFPRDEYFMRLALREAERALEHGDLPVGCVIAYEDEVI
                     AASPNERELRQDPTAHAEILALREASGKLGSWRLLETSMYVTLEPCAMCAGAIVLARV
                     PRVVWGAENPKAGAAGSVLDVLGEPRLNHRPVALGGLLSDESAELLRDFFADRR"
     gene            6420..6508
                     /locus_tag="VF752_09400"
     tRNA            6420..6508
                     /locus_tag="VF752_09400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6454..6456,aa:Ser,seq:cga)
     gene            complement(6509..7690)
                     /locus_tag="VF752_09405"
     CDS             complement(6509..7690)
                     /locus_tag="VF752_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8855806.1"
                     /translation="MSVRLKLTLSYAGFLMLAGALLLAAVWVFLLRYVPDRAVLVNPG
                     TDPLTSGVFPVRSNLLHVFAPRAAAVLAFLLVFGLLGGWILAGRMLAPLTRITDATRT
                     AATGSLSHRIRLPGRSDEFRELADAFDAMLARLEAHVAEQQRFAANASHELRTPLAIT
                     QTLLDAARNDQSRGHGELVDRLHAVNTRAIDLTEALLVLSRADQRSFAREHVDVSLIA
                     EEATETLLLLAEERGVTIETSGDMTPTIGSPALLLQMATNLVHNAIVHNLPGQGTVWV
                     TTSAHPKSVVLTVENTGEKLTPQLVATLAEPFRRGTQRVRTEHTGVGLGLAIVKSITQ
                     AHDGILTLTPRAAGGLCVTVQLPAAAPHLADDRQARPRLASTARAGGEIAAANRGGSL
                     L"
     gene            complement(7701..8396)
                     /locus_tag="VF752_09410"
     CDS             complement(7701..8396)
                     /locus_tag="VF752_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855807.1"
                     /translation="MRVLLVEDEPYMADAIRDGLRLEAIAADIARDGAAALELLIVNA
                     YDIAVLDRDIPGPSGDEIAKRIVASGSGMPILMLTAADRLDDKATGFELGADDYLTKP
                     FELQELVLRLRALDRRRAHNRPPVREIAGLRLDPFRREVSRDGRYVALTRKQFAVLEV
                     LVAAEGGVVSAEELLERAWDENADPFTNAVRITISTLRKRLGEPGIIATVPGAGYRID
                     TQRDTRREERDRG"
     gene            8483..9178
                     /locus_tag="VF752_09415"
     CDS             8483..9178
                     /locus_tag="VF752_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8855808.1"
                     /translation="MTYRQPAQTTTRRIRIRRIGVGGLLVVIAAIAAALGSQLLASSS
                     STATSPTDVPRSEHRGLRTEDGGAPGAPGALGERGALGEADGAIPDGTTVFDNEVPGV
                     AKLDPALLGALRRAATDAAGDGVEFYVDSGWRSPEYQERLLREAISKYGSEEEATRWV
                     ATPDTSAHVSGDAVDIGPSGAAAWLSEHGAEYGLCRIYGNEPWHYELRPEAIDHGCPH
                     VYADPTHDPRMQQ"
     gene            9255..9599
                     /locus_tag="VF752_09420"
     CDS             9255..9599
                     /locus_tag="VF752_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855809.1"
                     /translation="MRRKLRPLVALAMAALIGAGCSNGSAENGTTGTASHTGTSGNTG
                     TASNTGAGGHKNATDQDKIASPSSAGSPASPGSPPSPASSPSPGSPPSPGSRPSKGPY
                     AGFKDAAVISTR"
     gene            9667..10884
                     /locus_tag="VF752_09425"
     CDS             9667..10884
                     /locus_tag="VF752_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855810.1"
                     /translation="MRTALLAAVAATAALAGLAPSADASERLTRDVLVVSNNWAGTAD
                     LIDPHSFKRLKRLNVVPDRKQRIAEISADPTAKFYYDNIRALVGEGHNQYVDDGFTAP
                     DGRTVYFSRPSFADVVAMSLETGRIRWRTHIDGYRADHMAISRNGRRLLVSASTAGVI
                     DSIDTRTGGITARIPSGDSPHENNFSRDGSLIFHASIGTVYSDTDDPSQDATKGRRIF
                     EVIDARTNAVLKQIDMGQKLAEAGYPGMSSAVRPMAITPDERYVYFQVSFFFGIVQYD
                     LKADKVVAVLRLPLGAAKGVPRQQYLLDSAHHGLAMNPRGTRLCVAGTMSGYAAIVTR
                     KPFKLQRTTKVGRVPYWSQSSRDGKYCFVSVAGDDRVAVISYKTGLQVKRIKVGDHPQ
                     RMRTGVIRRAYLR"
     gene            complement(10859..>11569)
                     /locus_tag="VF752_09430"
     CDS             complement(10859..>11569)
                     /locus_tag="VF752_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8855811.1"
                     /translation="RTAARASVTGIEETSIRALVKLEQVLPQHLRRRVAALGSATVAP
                     AVDGPTVDPQHLTTIAAACRDLECLRFRYSSRDGSETRREVEPHTLVNLGRRWYLVAW
                     DRGRDDWRTFRVDRIDGPATTGVRFTSRELPAKDAATYVRENIAGAFTRFEARVTVHA
                     AAEDVAARLPQLAGALTPIDAQRCEYRTSDYDLEWLALRIAMLGVDVDVHEPPELVAQ
                     LRALVGRLRRATAGRRDG"
BASE COUNT         1800 a         3889 c         4082 g         1798 t
ORIGIN      
        1 ctgcgacgaa actcgagtcc ggctcgtcgt agacctcgtt cggcgctccc acctgaagca
       61 cgcgcccgtc gtccatgacc gcgatgcgat cggacatcgt gagcgcctcg tcctggtcgt
      121 gagtgacgta gacgaaggtg atgccgacgt ccgactggat ccgcttcaac tcgacctgga
      181 gctgctggcg cagcttgaga tcgagcgcac cgagcggctc gtcgagtagc agcacgcgcg
      241 gccggttcac gagcgcgcgt gcgagcgcga cgcgctggcg ctggccgccc gagagctgcc
      301 ccggccggcg cttgccatag tcctcgagcc gcaccatctc gagcgcctcg cgggcgcgct
      361 cgcggcgctc cgccttcccc acgcccttca cacgcagccc gtactccacg ttctcgccga
      421 cggacatgtg cgggaagagc gcgtagtcct ggaacaccgt gttcacgttg cgctcctcgg
      481 gcggcacctt cgtcacgtcc tgccccgcga gctcgatgcc cccggaatcg ggctgctcga
      541 agcccgcgat cagccgcagg cacgtcgtct tgcccgagcc cgatgggccg agcatggtga
      601 agaactcgcc ctcgcggatc tcgagactca cgtcgtcgac ggcggtcacg ccgccggcga
      661 aggtcttcct gagcccggag agccgtatgt cgacagaggc agccgacgcg ccctgggcca
      721 cgggcggggc agcggtctgc tccctcgtca tgtggcaggt tctacctgtt gtggaagaag
      781 cggtgtgacg ttccgtacaa atttgctccg ctgcggccaa ctgcggggtg agccctggga
      841 cgctggagcc aacggctaga gggtcagcgt gccgtcgatc accggcacga ctccgccggc
      901 gacctccact ctgtcctcca cgacccgcgc gctcagcctg ctcggcctac ccatttcgac
      961 accttgccga atggcgacgc tctcccagcc gagccgggcg ttcaggtacg cgcacaccgg
     1021 gccggcggca gagcccgtgg ccgggtcctc gcccatctcg gcggtcgcgg tgaaggcgcg
     1081 cgagcgcacc tcgccgtcgt cggctatcgc gaacacgtac agcgtgagcg cgccgatcgg
     1141 ctggagcagc gcgttcacga actcgtagcg cggctcgatc cgctccagcg cgtcgaggcc
     1201 gttgaccggc aagagcagct gcggcacgcc ggtggccacc acctgcgccg gcagctctgg
     1261 gtgcgcctcg cccggctcga gcgagaccgc cgccagcacc acgtcgggcg gcggctcctc
     1321 gccgaactcc ggcggctcct gcagcatcga ggcgatccaa gcgtcgccgt ccttgcggac
     1381 ctcgatcggt tggaggccgg cgtgggtctg ctgcacgtag cgcgccgtct cctcgccgcg
     1441 ggcgcgcgca accgccaccg cggtgccgag cgagggatgt cccgcgaacg gcacctcgcc
     1501 gctcatcatg aagatgcggt tgcggtagtc cgcgccgttg ttggtcgcgc tctgcacgaa
     1561 cgtggtctcg gatagccgcg tctcgcgcgc gaacgcgagc atcagcgact cgtcgagtcc
     1621 gtcggcgtcg tgcaccaccg ccaggccgtt cccctcgaat cgaacgtccg tgaaaacgtc
     1681 cacgaaggtg catggaagcg tggtcatgcg gccgcagtct tgcgcagcgc gcgcgtcgct
     1741 gcgtttgggg cgattttcga ggggtacgtc aagtacgacc gaccaaggag gttgatcatg
     1801 ggtttcctcg acaagctcac cggccggacc aagaaagccg caggcgacct gaccgacgac
     1861 ccttcgcttc gccgcgaagg ccgccaggag gagcggaagg gcgaggcgaa ggacgagctc
     1921 tcgaacgccc aggagcgcgc cgacgcgaag gcggacgagg tcgccgacct cgagcgcaag
     1981 accaactagg cgcccgctcg gcggccggcg ctcccgcggg ggcgccggct acgcgagctt
     2041 cacgatcgtg atcacggcgg agagcaagac gaagatcacg atcagcacct gcatcacgaa
     2101 ccaggagcgg ggcatgggcg gacggtcgtc catgcacccg aggctagtcg ttcgcgcgtt
     2161 cagtcgatca gcgactggcg aagcgcgatc gcgacagcgt gcgtgcgcgt gtcggcctgg
     2221 agcttcgcga tcacgcgctt cgtgtcggac ttgaccgtct ccacgctgag gccgatccgg
     2281 cgcgcgatct cttccgtgtg gtagccctcc gcgaggagct ggaggatctc gcgctcgcgc
     2341 tcggacagcg gctgctcggc ctgtgcggag ttggtcatga gcagcgccgg cgagagcgag
     2401 ggatccacga acgccttgcc gcccacgacc gtgcggatcg cgcgcagcag gtcctccggc
     2461 ggcgagccct tcatcacgta gccgcgcgcg ccggcggcga tcgcgtcgga cagcagacgc
     2521 ttgtccccgt aggcggagaa cacgaccagc gccacctcgg gcgcctcctc gtggatgcgc
     2581 ttgagcgtgg agatgccgtc gatgccggga aggcggacgt cgacgagcac cacctgcggc
     2641 ttcagccgct tcacggcggc gagcgcctcc tcgccggtgg tgaccgtgcc gaccagctcc
     2701 agatcgtcca actcgccgaa aaggagatcg agaccctgcc ggacaacggg gtggtcatcc
     2761 acgccgaggc agcgagtcct ggcggccatt tgtggatcct agctccgcca ctcaggcggc
     2821 gctagacctt ttgaggtagc cctgcaacat ggggtggagc cgggctccgg ggcgtttccg
     2881 catatgatgg ccgccgtctc agcttgctct tgggggaagg agaggaatgt tcggctccat
     2941 cgtggttggt accgacgggt ccgacactgc aaaggaggcc gtgcggcagg ccaccgtgct
     3001 cgcgaagagc acgggcgacg agctgctcgt ggtcagcgcc tacgaaccag tgcccgaggg
     3061 ccggctgcgc agcgagcgcc gcgacacccc cgaggacctg cagtggacca tcaacccgcg
     3121 tgaggacgtg gagacgacgc tcgaggacgc gagcgactcg atcggcgaga gcggcgtgaa
     3181 ggtgcagacg ttcgcgcgcg aaggggaccc ggccgacgca atcctcgacg tggcggagga
     3241 gcagaacgcc ggcctgatcg tcgtcggcaa caagggcatg accggcgcga agcggttcct
     3301 gctcggctca gtcccgaaca aggtgtccca tcacgcgcct tgctcggtcc tgatcatccg
     3361 gacgacctag ggcgcgaggg cggctgctac gtccagcaaa cccgcgccgt agcgggtgtc
     3421 gtagccgggc ggtccgaggt cgcgtgccgt acgcgcgagg cggtcctgta tgagggccgg
     3481 ggaggggcgc gagcccaggc gcttcgtcgc gatcagcagc gcggcagccg cggccacgtg
     3541 cgggctcgcc atcgaggtgc cctcgtactc cgagccgcgg accccgaagg tgcgaatgcc
     3601 gccgtcatgg cggaacgtga cctggaagat cggcggcaga tcgacgccgg ggtggcagtt
     3661 cgcctggtcg ctcgggttgt ccacgttggg cgcgtcattg ccgccgccgg gtgcggagat
     3721 gtcgaggccg ggaccggcat tcgagtagtc ggcctgacag aggtgctgcg tcgtcgcgcc
     3781 caccgagatg atctgccccg cacgcgccgg atacgcgacc gcggtgtccg cctcattgcc
     3841 ggccgcggcg accatggtca cgcccttgcg gtgcgcgtag cgcgcggccg agacgatctc
     3901 ggggatctgc gacgcggtga cggtggtgtc gaactcgagg ctcatgttga tcacccgtgc
     3961 gccgttattg gcggcgtagc ggatcgcgcg agagatcgcc gcggcgtcgc cggcgccctc
     4021 ggagtcgagc acgcgcagag gcatgatctt caccccgtaa gcgaggccca cgaggccgaa
     4081 gccgttgttg gtcgcctcgg cgatggtgga agtcacgtgg gtcccatggc cgttctcgtc
     4141 gttcgggtgg cggtcgttgt ccacgaagtc gaagccgcgg acgaagcgct gccggtagag
     4201 gtcaggcgac ttcttgaagc ggcggaagtt ctcgtacgcg acgccggtgt cgatcacggc
     4261 gaccactacg ccgcggccgc ccggcgcgcc ggcgcggatc gcctcgtccc acgcagcggg
     4321 ggcgttcacg ctcgccgggc cggcgagatc ccactggagc gcctgccagc cgccggcctg
     4381 gccggtcagg cccgggtcgt tcgggatgaa gctcgcgtga gccaggtagt tcggcaccgc
     4441 gtactccacg ctcgggtggc ggcgaagcgt ctgcacggtt tcgccgaccg tcttgctgtc
     4501 gcggatccgc aacgtgcgcg ggccgccagg cagcttgccc gcgtagctgg cgccggtctg
     4561 gcgctgcacc gccgcgcggg cggactttgc cactccgctg cggtatttca cgatcacctg
     4621 gcccggcgcg tagggcgccg cgcccgccga agcggaggct gcgagtagaa aggccgccgt
     4681 agcggcggcc gtcaggaggg ggcggcgggc gggcattcga ttacggtgca gcggcagagt
     4741 ctatgtcggc aagaaccctt caacaccgaa gttgtttctc aaaattgtcc cttaacgagg
     4801 tccgccagtg aagcgatctt gcgttaccgg ggccaccggg ttcgtgggcg cgcacgtggc
     4861 gcggatgctc gccgagcggg gccacgaggt ccgggtcacc taccgggacg agacgcggct
     4921 cactcgtctg agtgatacgg acgtcgagcc tgtgcgggcc gacgtgctcg accgcgcggc
     4981 gatgcgccgg gcagtgaggg gcgccgacgt gctctttcac accgcgggct acgtggcctc
     5041 gcgtccagcc gaccgcgtgt tcgagatcaa cgcgctctcc gtgcgcgtcg ccctcgaggc
     5101 ggcggccgcc gagggcgtag gccgggtggt cgtcacatcc agcgtgggcg cgctcgggcc
     5161 ggccgacgcc ggcgagctca tcgacgaggg acatctctac cgcggcacag ggctcgccta
     5221 cgccgacgcg aagcacgagg gcgaggccga ggcattcgcg gccgccgctc gcctgggagt
     5281 cgaggtggtc gtggtcgagc cggcgtacgt gctcggcgtg cccgtggacc atacgcagcc
     5341 gggcgagacc tccacgcgcg tgatcggcaa ctacctgcag ggacggttgc ccgccgtaat
     5401 ggagggcggg gcaagcttcg tggacgtgga cgacgtggcc gccggccacc tagcggccgc
     5461 cgaccgcggc gcgagcggcg agcgctacgt gctcgggggc cacaacctgg cgtggtgcga
     5521 gttgatcgag cgcgtcgccg agctctccgg agtgcgccac ccgatcgtcg tgctgccgcc
     5581 ggagatggag accgtcgcgc gcgtgcagcg gtcgctacgc cttccgttgc tgatctcgcc
     5641 ggaggcctac gcgctcatgg gggcggactg gcgctgctcc tcccgcaagg ccaagcgcga
     5701 gctcgactac ggcgtgcgtt cgctcgaccg gacgctccac gccacgatcg agtggtacca
     5761 ggagctgatc gaggcgggcg ccttccgcgg gcgggcaccg tcgtcgctct cgcttgcgtc
     5821 ggctggactg cgcgtggccg aacgcgccgg gctgttcccg ttcgtgcggg cggccgagcg
     5881 ctggagcggc aggcagctgc tggccggaac ccgcgaatga acgtcgaaag cgggttcttc
     5941 ccccgcgacg agtacttcat gaggctcgcc ctgcgcgagg cggagcgcgc gctcgagcac
     6001 ggcgacctgc cggtcggctg cgtgatcgcg tacgaggacg aggtgatcgc cgcctcgccg
     6061 aatgagcgcg agctgcgcca ggacccgacc gcgcacgcag agatactcgc gctgcgcgag
     6121 gcgtccggga agctcggcag ctggcggctt ctcgagacct ccatgtacgt cacgctcgag
     6181 ccctgcgcga tgtgcgccgg ggcgatcgtg ctggcgcgcg tcccgcgcgt tgtttggggc
     6241 gccgagaacc ccaaggccgg tgctgccggc agcgtcctcg acgtgctcgg ggagccgcgg
     6301 ctcaaccacc gcccagtggc cctcggcggg ctgctctcag acgagtcggc ggagctcttg
     6361 cgcgacttct tcgcggacag gcgctgaggc gcatttcggc tagccttgtc cgtcgcctcg
     6421 gaggggtgcc ggagtggttg aacggggcgg tctcgaaaac cgttgtgggt cgtaaggtcc
     6481 accgagggtt cgaatccctc cccctccgtt acaagagcga acctccgcga tttgctgcgg
     6541 cgatctcccc tcctgcgcgt gccgtcgacg cgaggcgcgg cctggcctga cggtcatctg
     6601 cgaggtgcgg tgccgcagct ggtagttgca ccgtgacgca gagcccaccg gcggcccggg
     6661 gggtgagggt gaggattccg tcgtgcgctt gggtgatgct cttgacgatt gccaggccga
     6721 ggccgacacc tgtgtgctcg gtgcgtacgc gttgcgtgcc gcgccgaaac ggctcggcaa
     6781 gcgtggcgac cagctgtggt gtgagcttct cgccggtgtt ctcgacagtg agcaccacgc
     6841 tcttggggtg agcgctcgtc gtgacccaca cggtgccctg tccaggcagg ttgtggacga
     6901 tcgcgttgtg cacgaggttc gtagccatct gcagcaggag ggcgggtgag ccgatggtgg
     6961 gggtcatgtc gccggaggtc tcaatggtga cgccgcgttc ttctgcgagg aggagaaggg
     7021 tttcggtggc ttcttccgct atgagggaca cgtcgacgtg ctctcgggcg aaggaccgct
     7081 ggtcggcgcg gctgagcacg agcaatgctt cggtgaggtc gatcgctcgc gtgttgacgg
     7141 cgtggaggcg gtcgacaagt tcgccgtggc cgcggctctg gtcgttgcgg gcggcgtcga
     7201 gcagtgtctg cgtgatcgcc agcggcgtgc gcaattcgtg ggaggcattg gctgcgaagc
     7261 gctgctgttc ggcgacgtgc gcttcgagcc gcgcgagcat ggcgtcgaag gcgtcggcga
     7321 gctcgcggaa ctcatcgctg cggcccggta gccggattcg gtgggagagc gatccggtcg
     7381 cagccgtgcg tgtggcgtcg gtgattcgag tcagcggggc gagcatgcgg ccggcgagaa
     7441 tccatcctcc caggagaccg aacaccagca ggaacgccaa caccgcggct gccctcggag
     7501 cgaaaacgtg caggaggttg gaccggacgg gaaaaacgcc actagtgaga gggtcggtgc
     7561 cggggttgac gaggaccgca cggtcgggga cgtaacgcag gaggaacacc cacacggcgg
     7621 cgagcagcaa ggcacctgcg agcatgagga acccggcgta gctgagggtg agtttgaggc
     7681 gaacgctcaa accagcggct ctatccacgg tctcgttcct cacgtcgggt gtctcgttga
     7741 gtgtcgatgc ggtagccagc gccgggcacg gtggcgatga tcccgggttc gccgagccgt
     7801 ttgcgcagtg tcgagatagt gatgcggacg gcgttggtga acgggtccgc gttctcatcc
     7861 cacgctcgct ctaagagctc ttcggcgctg acgacaccgc cttcggcagc gacgagaacc
     7921 tcgagcactg cgaactgctt cctggtcagc gcgacgtagc ggccgtcgcg ggagacctcg
     7981 cggcggaatg gatccagacg caggcctgcg atctctcgca caggcggcct gttgtgtgcg
     8041 cgcctgcggt cgagcgctct gagacggagc acgagttctt ggagttcgaa gggcttcgtg
     8101 aggtagtcgt cggcgccgag ctcgaacccg gtggccttgt cgtcgagacg atcggcggcg
     8161 gtcagcatga ggatcggcat gccgctgccg gaggcgacga tgcgtttggc gatctcgtca
     8221 ccggaggggc cggggatgtc tcggtcgagg acggcgatgt cgtaggcgtt gacgatcagc
     8281 agctccagag cggcggcgcc gtcgcgtgcg atgtccgccg cgatcgcttc taggcgtagg
     8341 ccatcgcgga tggcgtctgc catatagggc tcgtcctcga cgagcaacac gcgcatcctc
     8401 cgaatcgtag ggctggcgca tatcgtcggc atatcgaaaa ccgcatactt gctggcaaca
     8461 ccacgccacc ttcactgggg gcgtgaccta taggcaacca gcacaaacaa cgacccgtcg
     8521 gattcggatc cgcaggatcg gtgtcggcgg cctgctggtc gtcatcgcgg cgatcgccgc
     8581 agccctcggc tcccagttgc tggcgtcctc gtcgtcaacg gccacatcac ccaccgacgt
     8641 ccctcgcagt gagcaccgtg gtcttcgcac tgaggacggt ggtgcgccag gcgcgcctgg
     8701 tgcgctcggc gagcgtggtg cgcttggcga agctgacggt gccattcccg acgggacgac
     8761 ggtcttcgat aacgaggttc cgggtgtagc caagctcgat ccagctctcc tcggtgccct
     8821 gcgccgagct gcaactgatg ccgcaggcga cggggtcgag ttctacgtcg atagcggctg
     8881 gcgttccccg gagtaccagg agcgactcct ccgtgaggcg atctcgaagt acggctcaga
     8941 agaggaagcc acccgatggg tggccacccc cgacacgtct gcacacgtgt cgggggacgc
     9001 ggtcgacatt gggccctccg gtgccgcggc gtggctgtcc gagcacggcg ccgagtacgg
     9061 gctgtgccgg atctacggca acgagccgtg gcactacgaa ctccgcccgg aggccatcga
     9121 tcacggttgc cctcacgtgt acgcagaccc cacgcacgat ccaaggatgc agcagtgacc
     9181 agcacctcgg cacaaggagc tggtgtcaac gcaatcggtc acacgcgagg agaaacatga
     9241 gtacgaggag aaacatgaga agaaagctac ggccgctggt cgcccttgcc atggccgccc
     9301 tgatcggcgc gggctgttcg aacggatctg ccgagaacgg caccaccggc accgccagcc
     9361 acaccggcac ctccggcaac accggcaccg ccagcaacac cggcgccggg ggtcacaaga
     9421 acgccaccga tcaggacaag atcgcgtccc cgtcctcggc ggggtcgcct gcctcgccgg
     9481 ggtcgccgcc ctcgccggcg tcatcgccct cgccagggtc gccgccctcg ccggggtcgc
     9541 gcccttcgaa gggtccctac gcgggattca aggatgcagc ggtgatttcg acacgatgaa
     9601 cacgcgacga gcgcggtgct cgcggccgtc ggcgggccga ccgcgaggcg gttacgatca
     9661 gcagccgtgc gcaccgccct gctcgccgcc gtagccgcga ccgccgcgct cgccggcctc
     9721 gcgccgtccg ccgacgcatc cgagcgtctg acccgcgacg tgctcgtcgt cagcaataac
     9781 tgggccggca ccgcggacct gatcgacccg cacagcttca agcggctgaa gcggctgaac
     9841 gtcgtccccg accgtaagca acggatcgcc gagatctcgg ccgacccgac cgcgaagttc
     9901 tactacgaca acatccgcgc gctggtcggc gagggccaca accagtacgt cgacgacggt
     9961 ttcaccgcgc ccgacggcag gaccgtctat ttctcgcgcc cgagcttcgc cgacgtggtc
    10021 gccatgagcc tcgagacggg caggatccgc tggcgcactc acatcgacgg ctaccgcgcc
    10081 gaccacatgg ccatctcccg gaacgggcgc cggcttctcg tgtccgcgtc gaccgccggc
    10141 gtcatcgact cgatcgacac gcgtaccgga ggcatcaccg cccgcatccc ctcgggagac
    10201 tcgccgcacg agaacaactt ctcccgcgac gggtcgttga tcttccacgc cagcatcggg
    10261 accgtttaca gcgataccga tgacccatcg caggacgcga cgaagggccg gcgcatcttc
    10321 gaggtcatcg acgcccgcac caacgcggtg ctcaagcaga tcgacatggg ccagaagctc
    10381 gccgaggccg gctaccccgg catgagctcc gcagtccggc cgatggcgat tacccccgac
    10441 gagcgctacg tctacttcca ggtctccttc ttctttggga tcgtccagta cgacctcaag
    10501 gccgacaagg tcgttgccgt gctcaggctc cccctcggcg ccgcgaaagg cgtcccgcga
    10561 cagcagtacc tgctcgactc cgcccaccac ggcctggcga tgaaccctcg gggcacgaga
    10621 ctctgcgtgg ccgggacgat gtcaggctac gcagcgatcg tcacccgcaa gccgttcaag
    10681 ctccagcgca cgacgaaggt cggccgcgtc ccctactggt cgcagtccag cagggacggc
    10741 aagtactgct tcgtgtcggt cgcgggcgac gaccgcgtcg cggtcatctc ctacaagacc
    10801 ggccttcagg tgaagcgcat caaggtcggc gaccacccgc agcggatgcg caccggcgtc
    10861 atccgtcgcg cctacctgcg gtagcgcgcc gcagacgtcc tacaagggcg cgcagctgtg
    10921 cgacgagctc cggtggctcg tgcacgtcga cgtcgacgcc gagcatggcg atccggagag
    10981 caagccattc gaggtcgtag tccgatgtcc ggtactcaca gcgttgcgcg tcgatcggcg
    11041 tgagcgcgcc cgcgagttgg ggaagccgcg ccgcgacgtc ctcggctgcg gcatggacgg
    11101 tcacgcgcgc ctcgaagcgg gtgaaggcac cggcgatgtt ctcccgaacg tacgtcgcgg
    11161 cgtccttcgc gggaagttcg cgggacgtga accgcacgcc ggttgtggca ggcccgtcga
    11221 tccggtccac ccggaatgtg cgccagtcgt cgcggcctcg gtcccaggcg acgaggtacc
    11281 agcgccggcc gaggttcacg agcgtgtgcg gctcgacttc gcggcgcgtc tcgctcccgt
    11341 ctcggctgct gtagcggaac cggaggcact cgaggtcccg gcaggccgca gcgatcgtcg
    11401 tgaggtgctg cggatcgacg gtcggcccat cgaccgccgg cgcgactgtc gccgacccga
    11461 gcgcggcgac gcgacgccgc agatgctgcg gaagtacctg ctcaagcttg accagcgcgc
    11521 ggatcgaggt ctcctcgatg cccgtcacgg acgcccgagc ggcggtgcg
//
LOCUS       DASVXS010000168        11804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1389047, whole genome shotgun sequence.
ACCESSION   DASVXS010000168 DASVXS010000000
VERSION     DASVXS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389047"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VF752_09435"
     CDS             <1..1311
                     /locus_tag="VF752_09435"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX8855812.1"
                     /translation="NRSRGDGLSAAQTRRAAARAAALASASEQAPAALALRTAAATTG
                     GGSGDNDSSPIVRTVRDIVNVVPDWMKFLLAGLIALVLVLGAGSVVSTRRARRLSRQR
                     EELLEEVGLLQAALLPKVPAQLGELETTVAYRPAEGLAAGGDFYDAFELEDGRVAIVL
                     GDVAGHGRSALERTSLMRYTLRAYVEAGLEPRHAVQVAGRALDKELNGDFATVVVAIH
                     DPRKGTLTWAGAGHPPPIVLGASAHRPVTVESAPPIGLGLATGMRQTTVPAPAGTVAC
                     FFTDGLVEGRIGDGLLGRERLRDLLVELSPDDPASRLLDLVVETADRVTDDMATCIVR
                     ATHRSKAVPPQDRLEELEVEAGELDGDRPVRFLRACGVSQGVAAETVEAARIAAATSG
                     SAVLKVRVPLDRAIAPAVTVAAGEAAAVEPQPAGSPVVGDLPRA"
     gene            1397..2926
                     /locus_tag="VF752_09440"
     CDS             1397..2926
                     /locus_tag="VF752_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX8855813.1"
                     /translation="MPRSERDSSRLRCAIAACGALAALALAPPGVAAAQLFTPDSSFG
                     SGVQANGRFVDVESIAADPGGRIYVADAGARHVEVYDNAANGNRLLRVIGDGQLQQPV
                     GVALDNRQRLYVADAGRNSVLMYDSYVDNNGLRREFGGLGPAIGQMANPRLLAANQAA
                     QIYVVEHDNARVQWWEPSGGSQAPVAAFGTADPPTFNDPWGIALDQAAQRIFVSNASS
                     TDGAIRYYDPRGFPQGTLASPGSAPGQVNAPRGMAFDSVGRLIVADSGNDRVQVFNPL
                     AAGGGVLDVFGSHGGGAGQFDMPSDVTFAPGAKVYVADTDNGRIVRLRYDDADQDGAI
                     DARDNCRGLSNPDQADADRDGLGDACDPDMDNDGVPNASDRCPKTRRGIDLNHDGCGD
                     PRSRINSPRAKASLRKIRRLRISGTAAGDELGVAQVKVALARVRGTKCQWYQRGGRFA
                     RASACSAPKFVNATGTDRWSTTLKVRAKGTYRVLSRAVQAGGLQETAASKRNTIVFCV
                     R"
     gene            2958..3566
                     /locus_tag="VF752_09445"
     CDS             2958..3566
                     /locus_tag="VF752_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855814.1"
                     /translation="MLARHLIVCACALAALALPAAASAKFGQSFRPMPHNPADRLLSV
                     PIDDFRYDHATRCRKKPAPGALAMQSWLASHSRGVSWGIMRCEKLGPGNYSLHAEGRA
                     IDWHLDVRNRADRRAAEHLIDLLLAPDRAGNPFALARRMGVQEIIWNCQAWWGSEAMI
                     KYSLCYDAKDRPKKIDDTSAHRNHVHIGLSRAGAAKRTSFWR"
     gene            3699..3977
                     /locus_tag="VF752_09450"
     CDS             3699..3977
                     /locus_tag="VF752_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX8855815.1"
                     /translation="MCLAIPGQILEVVDEHNRLALVDVAGVRRNVNIGLLDAEDGGVG
                     PGDWVLIHVGFALSKVDEDEALATRKLLEQMGSEYEQELRELQASVIE"
     gene            4022..4267
                     /locus_tag="VF752_09455"
     CDS             4022..4267
                     /locus_tag="VF752_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX8855816.1"
                     /translation="MHADIHLAPACDHSHGCITCGDEGIPARVVEVDEAQELAVCAGE
                     DGSRSTVETALVGAVQVGDSLLVHAGTAIARLEEERA"
     gene            4264..5379
                     /gene="hypD"
                     /locus_tag="VF752_09460"
     CDS             4264..5379
                     /gene="hypD"
                     /locus_tag="VF752_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX8855817.1"
                     /translation="MRFVDEFRDADLGRALAGEILAAVESGRHYKVMEVCGGHTHSIY
                     KYGIDDLLPANVELVHGPGCPVCVIPMGRVDDGIAIAREPGVLFACFGDMLRIPGSDG
                     TLLDAKAEGADVRMVYSPLDALRLAKENPDREVVFFAVGFETTAPSTALTLKRAKAEG
                     LRNFFCMCNHVTIVPPLRALLESPDLRLDGFVGPGHVATVVGARPFEFIPAQYGRPVV
                     ISGFEPLDILQSIHMILRQLGEGRCEVENQYKRVVPYEGNLAALKVLAEVFELRPHFE
                     WRGLGFISQSALKLSDAYRDYDAEERYGVPGVRVADPKACQCGEVLKGVIKPWECKVF
                     GTACTPEHAIGTCMVSPEGACAAYYNYGRFARERAVV"
     gene            5382..6473
                     /gene="hypE"
                     /locus_tag="VF752_09465"
     CDS             5382..6473
                     /gene="hypE"
                     /locus_tag="VF752_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX8855818.1"
                     /translation="MADPAVSEREQKILGIIETARAKRAKFRDERITMAHGAGGRATQ
                     TLIEGLLVPAFGSETGAELADAASVSVNGSGLALTTDSFVVKPIRFPGGSIGELAVNG
                     TVNDLAVSGARPLALTLSLVLEEGLDSDELRFQVQAMADAARAAGVEIVAGDTKVVER
                     GHADSMYICTTGLGVRDERARLAPSALRPGDRILVSGRVGDHGTAIMLARGEFDLGAE
                     IESDTSSLWPAADVLLDAAGDSLRCMRDATRGGLASVLNELARASGVAMLVREAAVPV
                     HPAVAGAAEILGIDPMYVANEGKLAAFVAPDKADAALAALRSVPGCEEAAEIGEVRTE
                     PPGMVLVETGFGGRRVMDLLVGDPLPRIC"
     gene            6473..6832
                     /gene="hypA"
                     /locus_tag="VF752_09470"
     CDS             6473..6832
                     /gene="hypA"
                     /locus_tag="VF752_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX8855819.1"
                     /translation="MHELSIAESVVRIAANHANGRRVTKVELKVGHLRQVVPSALEFA
                     FELVAQGTPVEGAELEIEAVPAGGVCRSCGAETQLRDFPLQCAACGGLDVEVTRGEEL
                     LVDSLELEETFATSEGI"
     gene            6835..7917
                     /locus_tag="VF752_09475"
     CDS             6835..7917
                     /locus_tag="VF752_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855820.1"
                     /translation="MAVSVPEPKPHPKGEPYAPGVDEVHILWTSEGMSCDGDSVSITA
                     AGQPGIEDVLLGAIPGLPKVNLHNKVLSPTLGGEEFLAPFRAAVDGTLDAPFVLVVEG
                     SIPNEKINGDGYWTSMGNDPATGEPLTLNWWLDRLAPKAWAVIAVGTCATYGGIHAMA
                     GNPTGCMGLADYLGWDFRSQGGMPIVNVPGCPVQPENFMETLTWLLYQAAGLAPLIPL
                     DAQLRPTWIFGKTVHEGCDRAGYYEQGDFAKDYNSPKCLVKVGCWGPVVNCNVPKRGW
                     MGGIGGCPNVGGICIGCTMPGFPDKFMPFMDEPPGATLSSALSGGYGTMIRRLRGVTN
                     KTMNHEPKWRHNRPELTTGAEPRWRP"
     gene            7940..9736
                     /locus_tag="VF752_09480"
     CDS             7940..9736
                     /locus_tag="VF752_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX8855821.1"
                     /translation="MATIRERVKPEAGKVVDMAWDPITRIVGNLGIYTKIDFAQKEVV
                     QCKSTSSLFRGYSVFMKGKDPRDAHFITSRICGICGDNHATCSCYAQQMAYGVKPPPL
                     ADWIVNLGEAAEYVFDHTIFQDNLVFVDFCEQMVRDTNPSVMEKADKSEAPHADMHGH
                     RTIGDIMRSYNPFVGETYKEALQMSRITREMICLMEGRHVHPSTLYPGGVGTVATPTL
                     FTDYLSRLLRCFDFIKKAVAMNDDVFDFFYEALPGYEEVGRRRILLGCWGAFQDPYVC
                     DYDYRNMTKWGRAMFVTPGVVVDGELVTTDLVDINLGIRILLGSSYYEDWENEETFVE
                     RDPLGNPVDKRHPWNQTTIPKPQKRDLEGGNYSWVMSPRWHDNRTGDFLALDTGGGAL
                     ARLWTVALAGLVDTPYVKAPGDGTVRITLPQTPNTREEELVWRIPQWSNAIERDRARV
                     YFIAYGAAMAMYFLERGLDEVRAGRTKVFQDFEVPDEAIGCGFHEAVRGVLSHHVVIR
                     DKKIANYHPYPPTPWNGSPTDSFGTLGPYEDAVTGMPIFEENGPDDFRGIDIMRAVRS
                     FDPCLPCGVHMYLGNGRTVKQVHSPMFGAQHT"
     gene            9738..10199
                     /locus_tag="VF752_09485"
     CDS             9738..10199
                     /locus_tag="VF752_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855822.1"
                     /translation="MDEREIQERVARIEELLDQVDAIADPHDRETAGEIVQALLDLYG
                     EGLRRILMRADPKALADDDLVSHLLLLHGLHPVPPDARVRAAFSALGANAGRVELVRI
                     DELGVARVRLHGRDATLEIEAEQAVRTAAPDLEGMQVEDDGAIPLPVWQSA"
     gene            10232..10828
                     /locus_tag="VF752_09490"
     CDS             10232..10828
                     /locus_tag="VF752_09490"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEX8855823.1"
                     /translation="MASAPAPVRAPEERCELCGEAIEPEHRHLLDLESRELMCACRAC
                     CVLFDRRAAGGGHYRLVPERRLHLTGFELPDTQWDELRIPVEMAFFFHHSGEGRVVAF
                     YPGPMGATESLLDLRAWRSLEAANPVLEGLEPDVEALLVNRARGARDQWLVPIDECYA
                     LAGLIRSTWRGLTGGKEVWERIEGFFSELSERSTEWTT"
     gene            10816..11025
                     /locus_tag="VF752_09495"
     CDS             10816..11025
                     /locus_tag="VF752_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855824.1"
                     /translation="MDNVTTGKPDAKPDDSAHTPGVKEGNDPGGYRKMRGHLDDDRST
                     AKRSTGINPDMEEPIDPRMPNLSPG"
     gene            11035..11685
                     /locus_tag="VF752_09500"
     CDS             11035..11685
                     /locus_tag="VF752_09500"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEX8855825.1"
                     /translation="MASTVGSVLDLRFHVEDAATVEYAAVPTIGFDLRIESGRREIRS
                     LMLDVQVQIPARRRVHDPDDERLFDLFGDPSGWKDSLRTLPWMRTALVVPGFSGETVV
                     QLPMPCSYDFEVTAARYLASLEDGEVPLEFMFYGTAFYAGPGGLLQTAHVDWSSEAEY
                     RMPASVWHETMEHYFPRSAWLRVSKDTFDRLHAYRSKHALLSWERALDSLLDGKES"
BASE COUNT         1805 a         4024 c         4278 g         1697 t
ORIGIN      
        1 aatcgttcgc gtggcgacgg cctgtcggcc gcacagacgc ggcgagccgc ggcacgtgcg
       61 gccgcgctcg cgagcgcgtc cgagcaggct ccggccgcgc ttgcgctgcg caccgcggca
      121 gccactaccg gcggcggttc cggcgacaac gactcgtcgc cgatcgtccg caccgtccgc
      181 gacatcgtga atgtggtgcc cgactggatg aagttcctgc tcgcaggcct gatcgccctc
      241 gtgctcgtgc tcggcgccgg ctcggtcgtg agcacgcgac gcgcgcgtcg cctctcgcgt
      301 cagcgcgagg agctgctcga ggaggtcggc ctgctccagg cggctctgct gccgaaggtg
      361 cccgctcagc tgggcgagct cgagaccacg gtggcgtacc gcccggcgga ggggctggcg
      421 gcgggcggcg acttctacga cgccttcgag ctcgaagacg ggcgcgtggc gatcgtgctc
      481 ggcgacgtcg ccggccacgg ccgctcggcc ctcgagcgca cgtcgctcat gcgctacacg
      541 ctgcgcgcct acgtggaggc tgggctcgag ccgcgccacg cggtccaggt cgcgggccgc
      601 gcgctcgaca aggagctgaa cggcgacttc gccaccgtgg tcgtggcgat tcacgacccg
      661 aggaagggca cgctcacctg ggcgggcgcc gggcatccgc cgccgatcgt gctcggtgcc
      721 tccgcccacc gcccggttac ggtcgagtcg gcgcccccga tcgggctggg gctcgcgacc
      781 ggtatgcgcc agaccacggt tcccgcgccg gcgggcacgg tcgcgtgctt cttcacggac
      841 gggctcgtag agggccgcat cggcgacggc ctgctgggcc gtgagcgcct gcgcgacctg
      901 ctggtggagc tgtcgccgga cgacccggct tcgcgcctgc tcgacctggt ggtggagacc
      961 gccgatcgcg tgaccgacga catggccacc tgcatcgtcc gcgcgaccca ccgctcgaag
     1021 gccgtcccgc cgcaggaccg cctggaggag ctcgaggtgg aggccggcga gctcgacggc
     1081 gaccgcccgg ttcgcttcct cagggcctgc ggcgtgtctc agggcgtcgc ggcggagacg
     1141 gtcgaggccg cccgcatcgc tgccgccact agcggaagcg cggtgctgaa ggtgagggtc
     1201 ccgctggatc gggcaattgc tcccgcggtc acggtcgccg ccggcgaggc cgcggccgtc
     1261 gagccgcagc cggcgggcag cccggtggtt ggcgacctcc cgcgcgctta gagttccggg
     1321 cctcggcggg ggcggccacc gcaacctcgg cccaccgggc ttgtttgcgg tctggcgtcc
     1381 cttgccgctc atacgtatgc cccgctcgga gagagacagc tcgcgccttc gctgcgccat
     1441 cgccgcctgc ggcgcgctcg cggcactggc gctggcgccg ccgggcgtgg ccgctgcgca
     1501 gctcttcacc cccgacagct cgttcggctc gggcgtgcag gccaacggcc gcttcgtgga
     1561 cgtggagagc atcgccgccg accccggcgg gcggatatac gtggccgacg cgggggcgcg
     1621 gcacgtcgag gtgtacgaca acgccgcgaa cggcaaccgc ctgctgcgcg tgatcggcga
     1681 cggccagctc cagcaaccgg tgggcgtggc actcgacaac cgccagcgcc tctacgtggc
     1741 ggacgccggc cgcaacagcg tgctcatgta cgactcgtac gtggacaaca acggcttgcg
     1801 gcgcgagttc ggtggcctcg gcccggcgat cgggcagatg gcgaacccgc gcctgctcgc
     1861 ggcgaaccag gcggcgcaga tctacgtggt cgagcacgac aacgcgcgcg tccagtggtg
     1921 ggagccgtcg ggcggctcgc aggcgccggt cgcggccttc ggcaccgccg atccaccgac
     1981 cttcaacgac ccatggggca tcgcgctcga ccaggcggcg cagcgcatct tcgtctcgaa
     2041 cgcgagcagc actgacggcg cgatccgcta ctacgacccg cgcggattcc cacaggggac
     2101 gctcgcctcg ccgggcagcg ccccgggaca ggtaaacgcg ccgcggggga tggccttcga
     2161 cagcgtcggc cggctgatcg tggctgactc cggcaacgac cgcgtgcagg tgttcaaccc
     2221 gctcgcggcc ggtggcggcg ttctcgatgt gttcggcagc cacggcggcg gcgcgggcca
     2281 gttcgacatg ccgtccgatg tgaccttcgc gcctggcgcc aaggtctacg tggccgacac
     2341 ggacaatggc cgcatcgtgc gcctccgcta cgacgacgcc gaccaggacg gcgcgattga
     2401 cgcgcgcgac aactgccgcg ggctctcgaa ccccgaccag gccgacgcgg accgcgacgg
     2461 gctcggcgac gcctgcgacc cggacatgga caacgacggc gtgccgaacg cctccgatcg
     2521 ctgcccgaaa acgcgccgcg gcatcgacct caaccacgac ggctgcggcg acccccggag
     2581 ccgcatcaac tcgccgcgcg ccaaggcgag cctccgcaag atccgccgct tgcgcatcag
     2641 cggcacggca gcgggtgacg agctgggggt ggcgcaggtg aaggtggcgc tggcacgcgt
     2701 gcgcggcacc aagtgccagt ggtaccagcg gggaggccgc ttcgctcgcg cgagcgcctg
     2761 ctcggcgccg aagttcgtga acgcgacggg cacggatcgc tggtcgacga cgctgaaggt
     2821 gcgcgcgaag ggcacctacc gggtgctcag ccgcgcggtg caggccggcg gtctgcagga
     2881 gaccgccgcc agcaagcgca acacgatcgt cttctgcgtg cgttaaagcc ctccggcggc
     2941 ccgccgatga aggtgtcgtg ctcgcccgcc acctcatcgt ctgcgcgtgc gcgctcgcag
     3001 cgctcgcgct cccggccgcc gcgtcggcga agttcggcca gagcttccgc ccgatgccgc
     3061 acaacccggc cgaccgcctg ctgtcggtgc cgatcgacga cttccggtac gaccacgcca
     3121 cgcgctgccg caagaagccg gcgcctggcg cgctggcgat gcagtcgtgg ctcgcctcgc
     3181 actcgcgcgg cgtctcctgg ggcatcatgc gctgcgagaa gctcggcccg ggcaattact
     3241 cgctgcacgc cgagggccgc gcgatcgact ggcacctcga cgtgcgcaac cgcgccgatc
     3301 ggcgcgccgc cgagcacctg atcgacctcc tgctcgcgcc cgatcgcgct ggtaacccgt
     3361 ttgcgcttgc ccgtcgcatg ggggtacagg agatcatctg gaactgccag gcctggtggg
     3421 gcagcgaggc gatgatcaag tactcgctct gctacgacgc caaggaccgg ccgaagaaga
     3481 tcgacgacac gagcgcccac cgcaaccacg tccacatcgg gctctcgcgc gcgggcgcgg
     3541 caaagcgcac cagcttctgg cgctgacgcc tttcgccagc ttggcgtccg accactgcac
     3601 cacccggttg cccgggtcac ccaagggtgc taaaacgacc tttcggcgca ccgagtgcca
     3661 gccccggtgc cggaggcgtc gcggaaggga gccgcaccgt gtgcctggcc atacccggcc
     3721 agatacttga ggtggtcgac gagcacaacc ggctcgccct ggtggacgtg gccggcgtgc
     3781 ggcggaatgt gaacatcggc cttctcgacg cggaggacgg gggcgtgggt cccggcgact
     3841 gggtgctcat ccacgtgggc ttcgctctct cgaaggtcga cgaggacgag gctcttgcca
     3901 cgcgcaagct gctcgagcag atgggcagcg aatacgagca ggagctgcgg gagctccagg
     3961 cgagcgtgat cgagtgaagc cacggagggc aacacccggg acggtgcgga cgtgatctga
     4021 catgcacgcc gatatccacc tcgccccggc ttgcgatcac tcacacgggt gcatcacctg
     4081 cggcgatgag gggattccgg cgcgcgtggt ggaggtcgac gaggcgcagg agctggccgt
     4141 ctgcgcgggc gaggacggct cgcggtcaac ggtcgagacc gccctcgtcg gcgccgtgca
     4201 ggtcggcgac tcgctgctgg ttcacgccgg caccgcgata gcgcggctgg aggaggagcg
     4261 ggcgtgaggt tcgtcgacga gttccgcgac gcggaccttg ggcgcgcgct ggcgggcgag
     4321 atcctcgccg ccgtcgagtc aggccgccac tacaaggtga tggaggtctg cggcggccac
     4381 acgcactcga tctacaagta cgggatcgac gacctgctgc cggcgaacgt ggagctggtg
     4441 cacgggcccg gctgccctgt ctgcgtgatc ccgatgggcc gggtggacga cggcatcgca
     4501 atcgcgcgcg agccgggcgt gctcttcgcc tgcttcggag acatgctgcg catcccgggg
     4561 tccgacggca ccctgctcga cgcgaaggcc gagggcgccg atgtgcgcat ggtctactcc
     4621 ccgctcgacg cgctccggct cgctaaggag aaccccgacc gcgaggtcgt gttcttcgcg
     4681 gtcggcttcg agacgaccgc gccctccacg gcgctcacgc tcaagcgagc gaaggccgag
     4741 gggctgcgga acttcttctg catgtgcaac cacgtgacaa tcgtgccgcc gctgcgcgcg
     4801 ctgctcgaat cgcccgacct gcgcctcgac ggcttcgtgg ggccggggca cgtggcgacg
     4861 gtggtcggag cgcggccgtt cgaattcatc cctgcgcagt acgggcggcc ggtcgtgatc
     4921 tccggcttcg agccgctcga catcctgcag tcgatccaca tgatcctgcg ccagctcggc
     4981 gagggccgct gcgaggtgga gaaccagtac aagcgggtgg tcccgtacga ggggaacctg
     5041 gcggcgctga aggtcctcgc cgaggtgttc gagctgcggc cgcatttcga gtggcgcggg
     5101 ctcgggttca tctcgcagag cgcgctgaag ctctcagacg cgtaccgcga ctacgacgcc
     5161 gaggagcgct acggcgttcc gggcgtgcgg gtggcggacc cgaaggcctg ccagtgcggc
     5221 gaggtgctca agggcgtgat caagccctgg gagtgcaagg tgttcggcac ggcctgcact
     5281 ccggagcacg cgatcggcac gtgcatggtc tcccccgagg gagcctgcgc ggcctactac
     5341 aactacgggc gcttcgcgcg cgagcgggcg gtggtctgac cgtggccgat ccggcggttt
     5401 ccgagcgcga gcagaagatc ctcgggatca tcgagaccgc tcgggccaag cgcgcgaagt
     5461 tcagggacga gcggatcacg atggcgcacg gcgcgggcgg ccgcgcgacg cagacgctga
     5521 tcgaggggct gctcgtgccc gcgttcggct cggagaccgg cgccgagctg gccgacgccg
     5581 cgagcgtgtc ggtgaacggc agcgggctcg cgctcaccac cgacagcttc gtggtgaagc
     5641 cgatccgctt cccgggcggc tcgatcggcg agctcgcggt gaacggcacg gtgaacgacc
     5701 tggcggtgtc gggggcgcgg ccgctcgcgc tcacactgtc gctcgtgctg gaggagggac
     5761 tcgactccga cgaactgcgc ttccaggtcc aggcgatggc ggacgccgct cgcgcggcgg
     5821 gcgtggagat cgtcgccggc gacaccaagg tggtcgagcg gggacacgcc gactcgatgt
     5881 acatctgcac caccgggctc ggcgtgcgcg acgagcgcgc caggctggcg ccttcggcgc
     5941 tccgcccggg cgaccgcatc ctcgtgtccg gccgcgtcgg cgatcacggc acggcgatca
     6001 tgctcgcgcg tggcgagttc gacctcggcg cagagatcga gtcggacacc agctcgctct
     6061 ggcccgcggc cgacgtgctg ctcgacgcgg cgggcgactc gctgcgctgc atgcgcgacg
     6121 ccacgcgggg cgggctcgcg tcggtgctga acgagctggc gcgtgcgtcc ggcgtcgcga
     6181 tgctggtacg tgaggccgcc gtgccggtgc acccggcggt ggctggcgct gccgagatcc
     6241 tcggcatcga cccgatgtat gtggcgaatg agggcaaact ggccgccttc gtggcgccgg
     6301 acaaggccga cgccgccctc gccgccctgc gctctgtgcc gggctgcgag gaggcggccg
     6361 agatcggcga ggtgcgcacg gagccgccgg gcatggtgct cgtcgagacg ggcttcggcg
     6421 gccggcgcgt gatggacctc ctggtgggcg acccgcttcc gaggatctgc tgatgcacga
     6481 gctctcgatc gcggagtcgg tggtgaggat cgcggcgaat cacgcgaacg gcaggcgcgt
     6541 gacgaaggtt gagttgaagg tggggcatct gcggcaggtg gtgccatcag cgctcgagtt
     6601 cgcattcgag ctcgtggcgc agggcacgcc agtcgagggt gcggagctcg agatagaggc
     6661 ggtgcccgcc ggcggggtgt gccgcagctg cggcgcggag acgcagctgc gggacttccc
     6721 gctccagtgc gccgcctgcg gtggcctcga cgtggaggtc acacggggcg aggagctgct
     6781 cgtggactcg ctcgagctcg aggagacgtt tgcgacgagt gaagggatct agccatggcg
     6841 gtatcggtac ctgagcccaa accacacccg aagggtgagc cctacgcgcc cggagtggac
     6901 gaggtgcaca tcctctggac ctcggaggga atgagctgcg acggggactc ggtgtcgatc
     6961 accgccgccg gccagcccgg catcgaggac gtgctcctcg gcgcgatccc gggcctgccg
     7021 aaggtcaacc tccacaacaa ggtgctatcg ccgacgctcg gcggcgagga gttcctcgcg
     7081 ccgttccgcg ccgctgtgga cggcacgctc gacgccccgt ttgtgctcgt ggtggagggc
     7141 tcgatcccca acgagaagat caacggcgac ggttactgga cgtcgatggg caacgacccg
     7201 gcgacgggcg agccgctgac gctgaactgg tggctcgacc gcctcgcgcc gaaggcctgg
     7261 gcggtgatcg cggttggcac ctgcgccacc tacggcggca tccacgcgat ggccgggaac
     7321 ccgaccggct gtatgggcct ggccgattac ctcggctggg acttccgctc ccagggcggc
     7381 atgccgatcg tgaacgtgcc gggctgcccg gtgcagccgg agaacttcat ggagacgctc
     7441 acctggctgc tctaccaggc ggccgggctc gctccgttga tcccgctcga cgcgcagctg
     7501 cggcccacgt ggatcttcgg caagaccgtg cacgagggct gtgaccgcgc cggctactac
     7561 gagcagggcg acttcgcgaa ggactacaac tcgcccaagt gcctcgtgaa ggtgggctgc
     7621 tggggcccgg tcgtgaactg caatgtgccg aagcggggct ggatgggagg catcggaggc
     7681 tgcccgaacg tgggcggcat ctgcatcggc tgcacgatgc ccggcttccc ggacaagttc
     7741 atgccgttca tggacgagcc gccgggagcg acgttgtcgt cagcgctgag cggcggctac
     7801 ggaacgatga tccggaggct ccgcggcgtc acgaacaaga cgatgaacca cgaaccgaag
     7861 tggcgacaca accggccgga gctcaccacg ggcgccgagc cgcgctggcg cccgtagcgc
     7921 gtcaggaacg ggagaagaaa tggccaccat tcgagagaga gtcaagccag aggccggcaa
     7981 ggtcgtcgac atggcgtggg acccgatcac caggatcgtc gggaacctcg gcatctacac
     8041 caagatcgac ttcgcccaga aggaggtcgt gcagtgcaag agcacgtcct cgcttttccg
     8101 cggctacagc gtgttcatga agggcaagga ccctcgcgac gcgcacttca tcaccagccg
     8161 catctgcggc atctgcggtg acaaccacgc cacctgctcg tgctacgcgc agcagatggc
     8221 ctacggggtg aagccgccgc ccctcgcgga ctggatcgtg aacctcggcg aggccgccga
     8281 gtacgtgttc gaccacacga tcttccagga caacctggtg ttcgtggact tctgcgagca
     8341 gatggtgcgg gacacaaacc cgagcgtcat ggagaaggcg gacaagtccg aggctccgca
     8401 cgccgacatg cacgggcacc gcacgatcgg cgacatcatg cgctcctaca acccgtttgt
     8461 cggggagacg tacaaggaag cgctccagat gagccggatc acgcgcgaga tgatctgcct
     8521 catggagggg cgccacgtgc acccgtccac gctctacccg ggcggtgtag gcacggtggc
     8581 cacgcccacg ctgttcacgg actacctcag ccgcctgctg cggtgcttcg acttcatcaa
     8641 gaaggccgtc gccatgaacg acgacgtgtt cgacttcttc tacgaggccc tgccgggcta
     8701 cgaggaggtc gggcgaaggc gcatcctgct cggctgctgg ggcgcgttcc aggaccccta
     8761 cgtgtgcgac tacgactacc gcaacatgac caagtggggg cgcgcgatgt tcgtcacgcc
     8821 cggcgtggtc gtggacggcg agctggttac cacggacctc gtggacatca acctcggcat
     8881 ccgcatcctg ctcggcagct cgtactacga ggactgggag aacgaggaga cgttcgtgga
     8941 gcgggacccg ctcgggaacc cggtcgacaa gcggcatccc tggaaccaga cgaccatccc
     9001 gaagccgcag aagcgcgacc tcgagggcgg caactacagc tgggtgatga gcccgcgctg
     9061 gcacgacaac cgcacgggcg acttcctcgc gctcgacacc ggcggcggcg cgctcgcgcg
     9121 cctctggacg gtggcgctcg ccggcctggt ggacacgccg tacgtgaagg cgcctggtga
     9181 cggcacggtg cggatcacgc tgccgcagac gccgaacacg agggaggagg agctcgtctg
     9241 gcgcatcccg cagtggtcga acgcgatcga gcgcgaccgt gcgcgcgtgt acttcatcgc
     9301 gtacggcgcg gcgatggcca tgtacttcct cgagcgaggg ctcgatgagg tgcgcgcggg
     9361 ccggacgaag gtgttccagg acttcgaggt tccggacgag gcgatcggct gcggcttcca
     9421 cgaggcggtc cgcggagtgc tgtcgcatca cgtggtgatc cgggacaaga agatcgcgaa
     9481 ctaccacccg tacccgccga ctccgtggaa cggctcgccg accgactcct tcggcacgct
     9541 cggtccgtat gaggacgcgg tcaccggcat gccgatcttc gaggagaacg gccctgacga
     9601 cttcaggggg atcgacatca tgagggcggt gcggagcttc gacccgtgcc tgccatgcgg
     9661 ggtgcacatg tacctcggca acggcaggac ggtgaagcag gtgcactcgc ccatgttcgg
     9721 agcgcagcac acctgacgtg gacgaacggg agatccagga gcgggtagct cggatcgagg
     9781 agctgctcga tcaggtcgac gcgatcgcgg atccgcacga ccgcgaaacg gccggcgaga
     9841 tcgtgcaggc gctcctcgac ctctacggcg aggggctccg gcgcatcctc atgcgcgccg
     9901 atccgaaggc cctcgccgac gacgacctcg tgtcacacct gctgctcctg cacgggctgc
     9961 atccggtgcc gcccgacgcg cgcgtgcgcg cggccttcag cgccctcggc gcgaacgccg
    10021 gccgggtcga gctcgtacga atcgacgagc tcggagtggc ccgcgtgcgg ctccacggac
    10081 gcgacgcgac gctcgagatc gaggcggagc aggcggttcg cacggcggcg ccggacctcg
    10141 aagggatgca ggtggaggac gacggcgcga tcccgctccc ggtgtggcag tcggcatgag
    10201 cgccccgctc gcctcatcgc gccttcgccg catggcgtcc gctccggctc cggtgcgcgc
    10261 gccggaggag aggtgcgagc tgtgcggcga ggcgatcgag cccgagcacc ggcacctcct
    10321 cgacctcgag tcgcgcgagc tgatgtgcgc ctgccgggcg tgctgcgtgc tgttcgacag
    10381 acgcgcggcg ggcggtgggc actaccgcct cgtgcccgag cgccgacttc acctcacggg
    10441 cttcgagctc cccgacacgc agtgggacga gctgcggatc ccggtggaga tggcgttctt
    10501 cttccaccac tcgggcgagg ggcgcgtggt ggcgttctac cccgggccga tgggcgcgac
    10561 ggagtccctg ctcgacctcc gtgcctggcg gtcgctcgag gcggcgaacc ccgtgctcga
    10621 ggggctcgag cccgacgtgg aggcgttgct cgtgaaccgc gcacgcggcg cgcgcgacca
    10681 gtggctcgtg ccgatcgacg agtgctacgc gctcgccggc ctgatccgct cgacgtggcg
    10741 cgggctcacc ggcggcaagg aggtctggga acggatcgag ggcttcttca gcgagctctc
    10801 agagaggagt acggaatgga caacgtgacg acgggcaagc cggacgcgaa gcccgatgac
    10861 tccgcccaca ctccgggcgt gaaggagggg aacgacccgg gcggctaccg gaagatgcgc
    10921 gggcacctcg acgacgaccg ctccactgcc aagcgctcca ccgggatcaa cccggacatg
    10981 gaggagccga tcgacccgcg catgccgaac ctgtcgccgg gctaggaggc tggggtggcg
    11041 tcgacggtcg gctcggtgct ggatctgcgc ttccacgtcg aggacgcggc cacggtcgag
    11101 tacgcggccg tgcccacgat tgggttcgac ctgcgcatcg agagcgggcg ccgggagatc
    11161 cgctcgctga tgctcgacgt gcaggtgcag ataccggccc ggcggcgcgt ccacgacccg
    11221 gacgacgagc gcctcttcga cctcttcggc gacccctccg gctggaagga ctcgctccgc
    11281 acgctgccgt ggatgcgcac cgcgctggtg gtgcccggct tcagtggcga gacggtggtg
    11341 cagctgccga tgccgtgcag ctacgacttc gaggtcaccg cggcgcgcta cctggcgtcg
    11401 ctcgaggacg gcgaggtgcc actcgagttc atgttctacg gcacggcctt ctacgccggc
    11461 ccaggcggcc tgctgcagac ggcgcacgtg gactggagca gcgaggcgga gtaccgcatg
    11521 ccggcgagcg tgtggcacga gacgatggag cactacttcc cgcgcagcgc ctggcttcgc
    11581 gtgtcgaagg acacgttcga ccgcctgcac gcctaccgct cgaagcacgc gctgctgagc
    11641 tgggagcgcg cgctcgactc gctgctcgat gggaaggaga gctgagcgtg gacgcggtcc
    11701 gtcgcatcgc cgacgccgtc ctgtacgagg gctacatcct ctggccctac cgcacctcgg
    11761 cgctgaagaa ccagcggcgc tggaccttcg gaggcgtgta cccg
//
LOCUS       DASVXS010000169         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_871285, whole genome shotgun sequence.
ACCESSION   DASVXS010000169 DASVXS010000000
VERSION     DASVXS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871285"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /gene="rplM"
                     /locus_tag="VF752_09505"
     CDS             complement(<1..269)
                     /gene="rplM"
                     /locus_tag="VF752_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX8855826.1"
                     /translation="MKTFVATPANRERNWLVVDATGKTLGRLATQVADALRGKRKPEY
                     TPHCDTGDFVVVINAEKIAVTGNKRQDKLYWRHSGYPGGIRSRTL"
     regulatory      455..641
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            651..1856
                     /locus_tag="VF752_09510"
     CDS             651..1856
                     /locus_tag="VF752_09510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8855827.1"
                     /translation="MPTKRITLFAMLLAASAPAAASGQTGAGTGGTGFVPTPALAKVA
                     CVKGCASKARPRVGSTIRLSGSNLTAVSQVVFQGARGNRDDVTVRVRPRSDRSVMLRV
                     PAKASSGPLKAVAASVSSASTKPLSILPPAPPPPATGKLTPVPGPRDPGAPALETAIS
                     SNNAFAGSANGILFSYRVGGSTPVDVQVDVVSQADGSVVQTWTVPGVQPGVVKSLGWK
                     GNAVGGVAPEGRYAFRLTAKTPSGASARSAQAAQGDRDSFDLHHQIFPVRGRHNFGGA
                     GARFGAGRTGHTHQGQDVLANCGTRLVAAEGGTVRYKQYQSAAGYYLIIHGDESGYDY
                     GYMHLRAPSPFAPGDHVDTGEQIGNVGDTGDATACHLHFEMWTAPGWYSGGKPQDPLA
                     SLQSWDAFS"
     gene            complement(1967..2383)
                     /locus_tag="VF752_09515"
     CDS             complement(1967..2383)
                     /locus_tag="VF752_09515"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX8855828.1"
                     /translation="MSATKARVARTITNRLVNPFSRWMAERGLLPGTAVLETIGRKSG
                     LARRTPVGNGLRGDTFWIITEHGHRADYVKNIQANPRVRVKAGHHRWRAGTAQILEND
                     DYRERMRMLHRRANDSMVRLMGTEHLTIRVDLDPDG"
     gene            complement(2384..2668)
                     /locus_tag="VF752_09520"
     CDS             complement(2384..2668)
                     /locus_tag="VF752_09520"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8855829.1"
                     /translation="MGILSWIIWGLVVGAIARVLRPGRQDIGIVWTIVLGVAGSLIGG
                     FIATDLLDIADTNDFDFGSFLIAVGTSVLLLVIWEAVARRRGHDARQAEQ"
     gene            complement(2797..4416)
                     /gene="guaA"
                     /locus_tag="VF752_09525"
     CDS             complement(2797..4416)
                     /gene="guaA"
                     /locus_tag="VF752_09525"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX8855830.1"
                     /translation="MTQAVPGDTAARESGAVGGAIAADAQQRQDEVLVLDFGGQYSQL
                     IARRVRECGVFSELLPHHVALEEIQRRRPRGLILSGGPASVYSDGAPKLRQELLELGV
                     PVLGICYGMQAIALELGGRIESAPIGEFGRSELTVFEPGLLFAGLPAQQTCWMSHRDT
                     VYEAPPGFTALAASTESPVAAFEHVPRGLYGIQFHPEVVHTPYGTDVLRRFLDDVCGC
                     DMQWSAASVIEEQIEKIRAQVGDGRVICGLSGGVDSSVAALLVHRAIGDQLTCIYVDH
                     GMMRKNESDQVIAAFRDTFHVPLVAVDAEERFLERLAGVTEPEEKRKIIGTEFIRVFE
                     EEAGKLDNPKYLVQGTLYSDVIESGGGTGAATIKSHHNVGGLPEDLQFELVEPLRTLF
                     KDEVRAVGLELGLPERLVWRQPFPGPGLAIRIVSGEVNAERLSILRDADFILQEEIRA
                     AGLYRELWQSFCVLPADLRSVGVQGDERTYGYVVVIRAVTSDDAMTADWARLPYDLLE
                     RVASRMINEIQQVNRVTLDITSKPPGTIEWE"
BASE COUNT          669 a         1584 c         1511 g          689 t
ORIGIN      
        1 ttgagggtcc gggaccggat cccgcccgga tagccgctgt ggcgccagta gagcttgtcc
       61 tggcgcttgt tcccggtcac ggcgatcttc tccgcgttga tgacgacgac gaagtcgccc
      121 gtgtcgcagt gcggcgtgta ctcgggcttg cgcttgccgc gcagggcgtc ggcgacctga
      181 gtcgcgagcc ggccgagcgt cttgccggtt gcgtcgacca cgagccagtt gcgctcgcgg
      241 tttgcgggtg tggctacgaa ggttttcatc gcacagaaga agcgcgccgg acggccgaga
      301 ctagcgcctt ggcccgcctc ggcggggcgc ggtggaacgg attgcctccg cgagccgcgt
      361 gcagcggccc ggggaatagt ggcacacgca ctcggggacg accactctgg gcctgcgttt
      421 gccgcctgcg gccgtcgtta tactgccgcg ccgacttgca gacctgccga atccggccgg
      481 gccagctcgg ccggagcggg ggaaccagag tgagggccga gaggccctcg cggggcgaat
      541 cagaagggcc tgcgccatgc gtggcgaagg ctgctctctg tagcgccgct ctttcggcgc
      601 gagcccgtca gctaaccccg taggcctgag aaagagaggc ctcgcccgag gtgccgacga
      661 agcgcatcac gctgttcgcc atgctgctcg ctgcgagcgc cccggctgcc gcatcgggac
      721 agaccggcgc gggcaccggc ggcaccggct tcgtgccgac accggcgttg gcgaaggtcg
      781 cgtgcgtgaa aggttgcgcg agcaaggcgc gcccgcgcgt gggctcgacg atccggctat
      841 ccggcagcaa cctgaccgcc gtcagccagg tcgtgttcca gggcgctcgg ggcaaccgcg
      901 acgacgtgac ggtccgcgta cggccgcgca gcgaccgaag cgtgatgttg cgggtgcccg
      961 ccaaggcgtc gagcggccca ctcaaggcgg tcgcagcgtc cgtcagctct gcgtccacca
     1021 agccgctctc gatcctccca cccgcgccgc caccgcccgc gaccggcaag ctcacccccg
     1081 tgcccggccc gcgcgatccg ggcgccccgg cgctcgagac ggcgatcagc tcgaacaatg
     1141 ctttcgccgg ctccgccaac gggattctct tctcgtaccg cgtcggcggg tcgacgccgg
     1201 tcgatgtgca ggtggacgtg gtcagccagg ctgacggctc ggtggtgcag acatggaccg
     1261 tccccggcgt ccagcccggc gtcgtgaaga gtttgggctg gaagggcaac gcggttggcg
     1321 gtgtcgcgcc cgaaggccgg tacgcgttcc gcttgaccgc caagacgccg tctggcgcga
     1381 gcgcgcgaag cgcccaggcc gcacagggcg atcgcgactc cttcgacctg catcaccaga
     1441 tcttcccggt gcgcggccgc cacaacttcg gcggcgctgg cgcacgcttc ggcgcgggtc
     1501 gcacgggcca cacgcatcag ggccaggacg tactggccaa ttgcggcaca cgcctggtcg
     1561 cggccgaggg cggcacggtc aggtacaagc agtaccagtc agccgcgggc tactacctga
     1621 tcatccacgg cgacgagtcg ggctacgact acgggtacat gcacctgcgc gcaccatcgc
     1681 cgttcgcgcc cggcgaccac gtggacaccg gcgagcagat cggcaacgtg ggcgacaccg
     1741 gcgacgcgac ggcgtgccat ctccacttcg agatgtggac ggcgcccggc tggtactcgg
     1801 gcggcaagcc ccaggacccg ctggcttcgc tgcagtcctg ggacgccttc agctaggccg
     1861 gcggctgcaa accttcgctc tgctgcgtcc tcgggcgctc gcttggctgt cgccaagctt
     1921 cggccctggg tccttgcata gctcggtttt cgcccgccgg cagacgtcag ccgtccgggt
     1981 cgaggtcgac tcggatcgtc aggtgctccg tgcccatcag gcgcaccatc gagtcgttcg
     2041 cccgccggtg gagcatcctc atgcgctccc ggtagtcgtc gttctcgagg atctgggccg
     2101 tgcccgctcg ccagcggtgg tgcccagctt ttacgcgcac gcggggattc gcctggatgt
     2161 tcttcacgta gtcggcacgg tggccgtgct cggtgatgat ccagaacgtg tcaccgcgca
     2221 ggccgttgcc cacgggcgtg cgccgcgcca gtccactctt gcgcccgatc gtctccagca
     2281 ccgccgtgcc gggaagcaag ccgcgctccg ccatccagcg cgagaacggg ttcaccaggc
     2341 ggttcgtgat cgtgcgcgcc acgcgcgcct tcgtagcgga catctactgc tcagcttggc
     2401 gcgcgtcatg cccgcgcctg cgtgccaccg cttcccagat cacgagcaac aacaccgacg
     2461 tcccaacggc gatcaggaag ctgccgaagt cgaagtcgtt cgtgtccgcg atgtccagca
     2521 ggtccgtcgc gatgaagccg ccgatgagcg agcccgcaac accgagcacg atggtccaga
     2581 cgatgccgat gtcctgacgt cccggccgca gcacgcgggc gatcgcgccc acgacgagac
     2641 cccagatgat ccacgagagg attcccatgc gcgaaggcta cgggcttgtg agcgcgcgtg
     2701 ccggggagta acttccggca cgtgcctggc tgacgctgac ctctcgctta cgccttgtac
     2761 cccagaccgc cgccttctcg gtctaatggc gtgtgcttac tcccattcga tggtgccggg
     2821 cggcttgctc gttatgtcca gggtgacccg gttcacctgc tggatctcgt tgatcatgcg
     2881 cgatgccacg cgctccagca ggtcgtaggg cagccgcgcc cagtcggcgg tcatggcgtc
     2941 gtcggacgtg accgcgcgga tcacgaccac gtagccgtag gtgcgctcgt cgccctgcac
     3001 gccgacggag cgcaggtcgg ccggcagcac gcagaaggac tgccacagct cgcggtacag
     3061 gccggcagcg cggatctcct cctgcaggat gaagtcggcg tcgcgcagga tcgagagccg
     3121 ctcggcgttc acctcgccgc tcacgatccg gatcgccagg ccaggcccag ggaacggctg
     3181 ccgccacacg agccgctccg gtagcccgag ctccagcccc accgcccgca cctcgtcctt
     3241 gaacagcgtg cgcagcggct cgacgagctc gaactgcagg tcctcgggca agccaccgac
     3301 gttgtggtgc gacttgatcg tggccgcacc ggtgcccccg cccgactcga tcacgtccga
     3361 gtagagcgtg ccctgcacga ggtacttcgg gttgtcgagc tttccggctt cctcctcgaa
     3421 cacgcggatg aactccgtgc cgatgatctt ccgcttctcc tcgggctcgg tcacgccggc
     3481 gaggcgctcg aggaagcgct cctcggcgtc gaccgccacg agcggcacgt ggaaggtgtc
     3541 gcggaacgcg gcgatcacct ggtcgctctc gttcttccgc atcatcccgt gatcgacgta
     3601 gatgcaggtg agctggtcgc cgatcgcgcg gtggacgagc aacgcggcga ccgaggagtc
     3661 gactccgccc gaaaggccgc agatcaccct cccgtccccc acctgcgctc ggatcttctc
     3721 gatctgctcc tcgatcaccg aagcggcgct ccactgcatg tcgcagccgc agacgtcgtc
     3781 gaggaatcgg cgcagcacgt ccgtcccgta gggcgtgtgc acgacctcgg gatggaactg
     3841 gatgccgtag agcccgcgcg gcacgtgctc gaaggcggca accggcgatt ccgtcgaggc
     3901 cgcgagcgcc gtgaagcccg gcggcgcctc gtagacggtg tcgcggtggc tcatccagca
     3961 ggtctgctgc gcgggcaggc ccgcgaacag cagaccgggc tcgaacaccg tgagctcgga
     4021 gcggccgaac tcgccgatcg gcgccgactc gatgcgcccg ccgagctcga gcgcgatcgc
     4081 ctgcatgccg taacagatgc cgagcaccgg cacgcccagc tcgagcagct cctggcgcag
     4141 cttcggcgcg ccgtcgctgt acaccgaggc cggaccgccc gagaggatca acccacgcgg
     4201 gcggcggcgc tggatctcct cgagcgcgac gtggtgcggc agcagctccg agaagacgcc
     4261 gcactcgcgc acgcgccgcg cgatcagctg cgagtactga ccgccgaagt cgaggaccag
     4321 cacctcgtcc tggcgctgct gcgcgtccgc cgcgatcgcg ccaccgacgg cgccgctctc
     4381 gcgggccgcc gtgtcgccgg gaactgcctg ggtcactcga ctacgagagc gggatcaggg
     4441 gcaacgccgt tcg
//
LOCUS       DASVXS010000170         7750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1013475, whole genome shotgun sequence.
ACCESSION   DASVXS010000170 DASVXS010000000
VERSION     DASVXS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013475"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VF752_09530"
     CDS             complement(<1..501)
                     /locus_tag="VF752_09530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX8855831.1"
                     /translation="MTEKEKMLAGELYDASDPELVEGRRRARALLRAYTDGDDRATLE
                     ELFARVGKDAFVQPPFACDYGSNIILGERSFVNFNGVFLDCAQISIGDRVEIGPNVQL
                     LTADHPRDVESRRKRLEFAKPIMIEDDTWVGGGAVVLPGLTVGAGSIVGAGSVVTRDV
                     PPGVIAA"
     gene            complement(498..974)
                     /locus_tag="VF752_09535"
     CDS             complement(498..974)
                     /locus_tag="VF752_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8855832.1"
                     /translation="MSKVSRDELIGRFIAAVRASQTATQTLDAAVADFLGIHGTDYRA
                     LDVLDQEGPMTAGQLAERARLSPGATTALIDRLEEKGLVRRIRDTEDRRRVQVDVRPE
                     LQEKAAQLYGSPEEGADELAGYSDEELEFLIGFLQRSVAYQEERMRRLEAIKASAV"
     gene            1038..2009
                     /locus_tag="VF752_09540"
     CDS             1038..2009
                     /locus_tag="VF752_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855833.1"
                     /translation="MKAIQASDLKKTYKRGVRALDGLSLEVEAGTIFALLGPNGAGKS
                     TTVKILTTLSRADSGEARVAGLDVVADAEAVRRSIGVVGQRPGLDGEATGRENLQMQA
                     EIYGIGGAAQRTRVQELLERVGLAPAADRLAKTYSGGMQRRLDVALGLVHRPKVLFLD
                     EPTTGLDPEARVELWQEIERLARDEEMTILLTTHYLEEADRLSSQLAIVDEGRVVAEG
                     SPDDLKRDLHGDAVVIELAESPNGDATAALDQLPGVSELTLEGRVLRARAANGAAAIP
                     AVITALEGRGLSIASATIARPSLDDVYLRHTGRSFQEADPYPKEIAA"
     gene            2006..2779
                     /locus_tag="VF752_09545"
     CDS             2006..2779
                     /locus_tag="VF752_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855834.1"
                     /translation="MIPLKHTWQVALRYIRALLRQPAWVGISLVQPAIWLLLFGALFK
                     RTADIPGFTGGSYIEFLTPGVIVMLAVSSAGWVGMGFIEDINRGTMDRMLVSPIWRGA
                     LNLGSVAQSVLSIAVQSVIVIGLSLLVGAHFHNGVVGVLVLIAVAGLLGAVFASLSNG
                     VAVIARQRETLIGVVTMVTLPLTFLSSALMQKSLLPEWIQSAASVNPINWAAEAGRSA
                     SSTHADWGLIASRCGLLVLLLAASAAFATRAFASYQRSI"
     gene            complement(3013..3483)
                     /gene="rpsG"
                     /locus_tag="VF752_09550"
     CDS             complement(3013..3483)
                     /gene="rpsG"
                     /locus_tag="VF752_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX8855835.1"
                     /translation="MPRRAAAGVRPIEPDALYGSKLVQQVINKVMIDGKKSTAEQIVY
                     DALDLLSRRAGSEPVEALETSIQKLTPNLEVRSRRVGGATYQVPVEVPSRRARTLAVR
                     WLVEFSRARREKTMAERLANELLDAQNDQGGAFKRKEDIYRMAQANKAFAHYRW"
     gene            complement(3494..3889)
                     /gene="rpsL"
                     /locus_tag="VF752_09555"
     CDS             complement(3494..3889)
                     /gene="rpsL"
                     /locus_tag="VF752_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX8855836.1"
                     /translation="MPSINQLVRKGRKKPKKKVATPGLKSGQGRKKAIAAPQRRGVCT
                     RVYTTTPKKPNSALRKVARVRLTNQIEVTAYIPGEGHNLQEHSVVLVRGGRVKDLPGV
                     RYKVVRGTLDAAGVSDRKKARSQYGVKAR"
     gene            complement(3930..4406)
                     /locus_tag="VF752_09560"
     CDS             complement(3930..4406)
                     /locus_tag="VF752_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8855837.1"
                     /translation="MSSKLIETAPGRVPVVGLLNRSGQLMDERLFSDLRAAGYPELRP
                     GHACVFGNIGRGGSRLTDIAERAQITKQAVGEVASELERIGYLERIPDPTDKRAKILR
                     LTKRGQEAQDAGYAIIQRVERELAEAYGVDFVNALRAALEDYTALGVGHAVGRHAA"
     gene            4547..6124
                     /locus_tag="VF752_09565"
     CDS             4547..6124
                     /locus_tag="VF752_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855838.1"
                     /translation="MERKWWTLIAASIATFMLLLDITVVNTALPDIERDLGASFTDLQ
                     WVVDAYTLSLAAFVLTAGSLADRIGRKRTFVTGLGIFTLSSLFIALAPNATFLNIGRA
                     VQGIGGAIMFALSLALIAQEFRGGRERGTAMGVYGGTIGIAVAIGPLVGGALTDSLGW
                     RSVFYLNVPVGLAAIGMTMARVAESRDPAARRIDWAGLGLFSGALFALVLALIRGNAE
                     GWGSSLIVGLFIVAAVLLTTFVLVERRVTEPMLPLSLFRIPAFTGVQLAAFGLSASMF
                     AIFLYLTLFLQNELGFGPLEAGIRYLPLTVASFLIAPLAGSLIGRVQTRVLIGLGLGL
                     TGAALVISSSLTTSSGWTALLPGFILAGLGVGMANPAIADVALSSVPKERAGMAAGVN
                     DAFRQVAISLGVATWGALFLSRGADKIAKLTAGTPASEGSNPRHLIEAASSGHVDQAV
                     RSLPAGSRDQVTAAAHQGFIAGLNDILLISGFVAFAGAIAALWLIRERDLERVDVEPV
                     EEPERVDTARVPEAAAA"
     gene            complement(6233..7111)
                     /locus_tag="VF752_09570"
     CDS             complement(6233..7111)
                     /locus_tag="VF752_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855839.1"
                     /translation="MTLELGILLALFCALATNLGFLYKHRGACAAPDVDWRHPLRSAV
                     GLWSSKWFAIGMFVAVGAWLLHVGAMAMAPLSLVQSVISGGLVFLTVLAERFFGFKVG
                     KRQWIGVGLMAAGLALLTVVLPASGGAHSAYSQAAMIAFESGLLVVGTLLVLSPKFGA
                     SHEHHGVLLGSAAGLLFGVSDVSIKALTGLVGTHGMVGLISPWLGAALLASVIAFYAS
                     ARGFQTGEAVPVITLTSAAANISAIGGGILVFGDPMPTHPLGIALQSVAFLLVIVAAA
                     VTPAPVRAANQAPATA"
     gene            7314..>7750
                     /locus_tag="VF752_09575"
     CDS             7314..>7750
                     /locus_tag="VF752_09575"
                     /inference="COORDINATES: protein motif:HMM:NF037735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HEX8855840.1"
                     /translation="MISLFGFPADGPPPGRFHWVDGVAHCIDEACGERIGRLWDAYEV
                     VWATGWEEKANEYLPHILSLPARELPVVSFEDRAPLASAHWKLEAIAEYAGERPAAWV
                     DDSLDAECERWARERRAPTLLVHTRPEIGLREQDVDVLLTWGD"
BASE COUNT         1195 a         2628 c         2643 g         1284 t
ORIGIN      
        1 cgcggcaatc acacctggcg gcacgtccct cgtcaccacg ctgcccgcgc ccacgatgct
       61 gccggcgccc accgtgaggc ctggcagcac gacggcgccg ccgccgaccc acgtgtcgtc
      121 ctcgatcatg atcggcttcg cgaactcgag ccgcttgcgc cggctctcga cgtcccgcgg
      181 atggtcggcg gtgagcagct ggacgttcgg cccgatctcg acgcggtcgc cgatgctgat
      241 ctgagcgcag tcgaggaaca caccgttgaa gttcacgaac gagcgctcgc cgagaatgat
      301 gttggacccg tagtcgcagg cgaacggcgg ctggacgaag gcgtccttcc ctacgcgcgc
      361 gaacagttcc tcgagcgtcg cgcggtcatc gccgtccgtg tacgcgcgta acagagcgcg
      421 ggctcggcgc cgaccctcga cgagctcagg gtcgctcgcg tcgtacagct cgccggcgag
      481 catcttctcc ttctcggtca cacggccgag gccttgatgg cctcgaggcg ccgcatgcgc
      541 tcctcctggt aggcgacgct gcgctggagg aaaccgatca ggaactcgag ctcctcgtcc
      601 gagtagccgg cgagctcgtc cgctccctcc tctggagagc cgtagagctg agccgctttc
      661 tcctggagtt cgggcctgac gtcgacctgg acgcggcggc ggtcctctgt gtcgcggata
      721 cggcgcacga ggcccttctc ctcgaggcga tcgatcagcg cagtggtcgc gcccgggctg
      781 agacgcgccc gctccgccag ctggcctgcg gtcatcggcc cttcctggtc gagcacgtcg
      841 agcgcgcggt agtcggtgcc gtggatgccg aggaaatctg cgacggctgc gtcgagcgtc
      901 tgagtcgcgg tctgagacgc gcgcaccgcc gcgatgaacc ggccgatcag ttcatccctc
      961 gaaacttttg acaatcgaat aacctctgtg ctagaacgtt tccaccatcg aaatatacct
     1021 ctcccctgga ggaaacggtg aaggcaatcc aagcatccga tttgaagaag acgtacaagc
     1081 gcggcgtccg cgcgctcgac ggcctcagcc tcgaagtcga ggccggaacg atcttcgcac
     1141 tgctcggtcc gaacggagcg ggtaagtcga cgacggtgaa gatcctcacg accctctcgc
     1201 gcgccgacag cggcgaggcc agggtcgcgg ggctcgacgt ggtggctgac gccgaggcgg
     1261 tgcggcgctc gatcggcgtc gtgggccaga ggcccggcct cgacggggag gccacgggcc
     1321 gcgaaaacct gcagatgcag gccgagatct acgggatcgg cggcgccgcg cagcgcacgc
     1381 gcgtgcagga gctactcgag cgcgttggcc tcgcgcccgc tgccgaccgg ctcgccaaga
     1441 cgtactcggg cggaatgcag cggcggctcg acgtcgccct ggggctcgtc caccggccga
     1501 aagtgctctt cctcgacgag cccacgaccg ggctcgaccc ggaggcgcgc gtagagctgt
     1561 ggcaggagat cgagcggctc gcccgcgacg aggagatgac gatcctgctc accacgcact
     1621 acctggagga ggccgaccgc ctgtcctctc agctcgcgat cgtcgacgag ggacgcgtgg
     1681 tcgccgaggg cagcccggac gacctgaaac gcgacctgca cggcgacgcc gtcgtgatcg
     1741 agctcgcgga gtcgccgaac ggcgacgcga ccgccgccct cgaccagttg cctggggtga
     1801 gtgagctcac gctcgagggc cgggtgctgc gtgcccgtgc ggcgaacggc gcggcggcca
     1861 tacccgcggt gatcaccgca ctcgaggggc gcgggctgtc aatcgcgtcc gccacgatcg
     1921 cccgcccctc gctcgatgac gtgtacctgc gccacaccgg acgctcgttc caggaggcag
     1981 acccctaccc gaaggagatt gcagcgtgat acccctcaag cacacctggc aggtagctct
     2041 gcgctacatc agagcgctct tgcgccagcc ggcctgggtc ggcatctcgc tcgtgcagcc
     2101 ggccatctgg ctgctcctgt tcggagcgct cttcaagcgc accgccgaca tcccgggctt
     2161 caccggcggt tcgtacatcg agttcctcac cccgggcgtg atcgtgatgc tcgcggtctc
     2221 gtccgcgggc tgggtcggga tggggttcat cgaggacatc aaccgaggca ccatggaccg
     2281 gatgctcgtc tcgccgatct ggcgaggggc gctgaacctc gggagtgtcg cgcaatcggt
     2341 gctcagcatc gcggtgcaga gcgtgatcgt gatcggtctc tcgctgctgg tcggcgccca
     2401 cttccacaac ggcgtggtcg gagttctggt gctgatcgca gtcgccgggc tgctgggcgc
     2461 cgtgttcgcc tcgctgtcca acggcgtggc ggttatcgca cggcagcgcg agacgctcat
     2521 cggcgtcgtc acgatggtga cgctgccgct cacgttcctg tcgtctgccc tgatgcagaa
     2581 gagcctgctg cccgaatgga ttcagtcggc ggcgagcgtg aacccgatca attgggcggc
     2641 cgaggcgggc cggtcggcgt cgagcacgca tgctgactgg ggcctgatcg cctcccggtg
     2701 cggtctcttg gtgctcctgc tggcggcgag cgcggcgttc gcgacgcggg cgttcgcttc
     2761 gtaccagcgg tcgatttagc gccggcgttt acagcagcaa tgagggtgag cccgggcgag
     2821 actgattttg ggccccctat atggggctca acttcagttt cgctccgggc aagagtcttt
     2881 gcgggcccgg aaagccggcg aaaaccgagc tatgcaagga cgcagggccg aagcttggcg
     2941 actagccaag cgagcgcccg aggacgccgc agagcgaagg tttgcaggcg gctcgcccgc
     3001 ggagagcggg acctaccacc ggtaatgcgc gaaagccttg ttagcctgag ccatgcggta
     3061 gatgtcttct ttgcgcttga acgccccacc ctggtcgttc tgcgcatcga gcagctcgtt
     3121 ggcgagccgc tcggccatgg tcttctcgcg cctcgcgcgc gagaactcca cgagccagcg
     3181 caccgctagc gtgcgggcgc ggcgagaggg gacctctacc ggcacctgat aggtggcacc
     3241 cccgactcgg cgggagcgga cctccaggtt gggggtgagc ttctgaatcg aagtctcgag
     3301 cgcctcgacg ggctcgctgc ccgcgcggcg tgacaacaga tcgagcgcgt cgtagacgat
     3361 ctgctcggcg gtcgacttct tgccgtcgat catcaccttg ttgatgacct gctggacgag
     3421 cttcgacccg taaagggcgt cgggctcgat cggacggact cctgctgcgg cgcgacgtgg
     3481 cactggcggt ctcctacctg gccttgacgc cgtactgcga gcgggccttc ttgcggtccg
     3541 agacgccggc ggcgtcgagc gtgccgcgga cgaccttgta gcggacgccc ggaagatcct
     3601 tcacgcggcc accgcgcacg agcacgacgg agtgctcctg gaggttgtga ccctcgcccg
     3661 ggatgtaggc ggtcacctcg atctggttcg tgagccggac gcgcgcgacc ttgcggaggg
     3721 ccgagttcgg cttcttcggc gtggtggtgt agacgcgcgt gcagacgcca cggcgctgcg
     3781 gcgcagcgat ggccttcttg cggccctggc cggatttgag gcccggcgtc gcgaccttct
     3841 tcttgggctt cttgcggccc ttgcgtacga gctggttgat gcttggcatg cggcgcgtga
     3901 tgctagcagc ggaacgcggc tgcgccggat caggccgcgt ggcggccgac ggcgtgaccc
     3961 acgcccagag cggtgtagtc ctcgagggct gcacgcaggg cgttgacgaa atccacgccg
     4021 taggcctcgg cgagctcccg ctctacgcgc tggatgatcg cgtagcccgc gtcctgcgcc
     4081 tcctggccgc gcttggtgag ccgaaggatc ttggcgcgct tgtccgtcgg atcggggatc
     4141 cgctcgaggt agccgatccg ctcgagctcg ctcgccacct cgccgacagc ctgcttcgtg
     4201 atctgcgcgc gttccgcgat gtcggtgagg cgagacccgc cgcgcccgat gttcccgaag
     4261 acgcacgcgt gcccggggcg aagctccgga tagccggcgg cacgcaggtc ggagaacagc
     4321 cgctcgtcca tcagctgccc cgacctgttc agcaggccga cgaccggcac ccgcccgggg
     4381 gcggtctcta tgagcttcga ggacatgggt tcggctatcc cccgtatttc gtctgatcgg
     4441 ttgacatggt caggtcgctt gataaggtcg ccgtaccagt tcgtcaggca gccttacaag
     4501 gcttcctgac aatcttagct cccgatcccc caggaaccaa accccgatgg aacgcaagtg
     4561 gtggaccctc atcgcggcgt cgatcgccac gttcatgctc ctgctggaca tcaccgtggt
     4621 gaacacggcg ctgcccgaca tcgagcgtga cctcggcgcg agcttcaccg acctccagtg
     4681 ggtggtcgac gcctacacgc tgtccctggc ggccttcgtg ctgacggccg gctcgctcgc
     4741 cgaccggatc gggcgcaagc gaacctttgt cacggggctc gggatcttca cgctctcgtc
     4801 gctcttcatc gcgctggcgc ccaacgccac gttcctgaac atcggccgtg cggtgcaggg
     4861 catcggcggc gcgatcatgt tcgccctgtc gctcgcactg atcgcccagg agttccgcgg
     4921 cggccgcgag cgcggcacgg cgatgggcgt ctacggcggc acgatcggca tcgccgtcgc
     4981 gatcggcccg ctcgtcggcg gcgcattgac cgactcgctc ggctggcgct cggtcttcta
     5041 cctgaacgtc ccggttggac tggccgccat cggtatgacg atggccaggg tggccgagtc
     5101 tcgcgacccc gcggcacggc gtatcgactg ggccgggctc ggcctcttca gcggcgccct
     5161 gttcgcccta gtgctcgcgc tgattcgcgg caacgcggaa ggctggggca gcagcctcat
     5221 cgtcggcttg ttcatcgtgg cggctgtgct gctcaccacg ttcgtgctcg tggagcgccg
     5281 cgttaccgag ccgatgctgc cgctctcgct gttccgaatc cccgcgttca cgggcgtgca
     5341 gctcgcagcg ttcgggctgt cggcctcgat gttcgcgatc ttcctctacc tcacgctgtt
     5401 ccttcagaac gagctgggct tcggaccgct cgaagcgggg atccggtacc tgccgctcac
     5461 cgtggcttcg ttcctgatcg ccccgctggc aggctcgctg atcggcaggg tgcagacgcg
     5521 cgtcctgatc ggcctgggcc tcggcctcac tggcgccgcg ctggtgatct cgagctcgct
     5581 gacgacgagt tccggctgga cggcgctcct gcccggcttc atcctcgcgg gactcggtgt
     5641 tggcatggcc aacccggcga tcgccgacgt agccctcagc tccgtcccga aggagcgcgc
     5701 gggaatggcg gccggcgtga acgacgcctt ccgccaggtg gcgatctcgc tcggcgtggc
     5761 gacctggggc gccctgttcc tcagccgcgg cgccgacaag atcgcgaagc tcacagccgg
     5821 cacccccgcc tccgagggct cgaacccgcg ccacctgata gaggccgctt catccggcca
     5881 cgtcgatcag gcggtccgga gcctgcccgc gggcagccgc gatcaggtca ccgccgccgc
     5941 ccatcagggc ttcatcgcgg ggctcaacga catcctgctg atctcggggt tcgtggcgtt
     6001 cgccggagcg atcgcggccc tgtggctgat ccgcgaacgt gacctcgagc gcgtggacgt
     6061 cgagccagtc gaggagccgg aacgggtcga caccgcacga gtgccggagg ccgccgccgc
     6121 ctgatcgtgt gacgcggcga aaaccgagtc aggcaaggac gcagggccga agcttggcga
     6181 tagccaagcg agtgcccgag gacacagcct gacgatggtt tgcagccgtg tcctaggcgg
     6241 tagccggcgc ctgattcgca gcgcggacgg gcgccggcgt aacggccgcc gccacgatca
     6301 cgagcaggaa ggcgaccgac tggagagcga tgccgagcgg atgcgtgggc atcgggtcgc
     6361 cgaacacgag gatcccgccg ccgatcgccg agatgttggc ggcggcactc gtaagggtga
     6421 tgaccgggac ggcctcgccg gtctggaatc cgcgcgccga ggcgtagaag gcgatcaccg
     6481 acgcgagcag cgcggcgcca agccacgggg agatcagccc gaccattccg tgcgtgccga
     6541 cgagaccggt gagcgccttg atcgagacgt ccgagacgcc gaacagcagt ccggcggccg
     6601 agccgagcag cacgccgtgg tgttcatggc tcgctccgaa cttcggcgag agcacgagga
     6661 gcgtgccgac cacgagcagg ccgctctcga aagcgatcat cgccgcctgc gagtaggccg
     6721 agtgggcgcc gccgctcgcg ggcagcacca cggtgaggag cgcaagcccg gcggccatga
     6781 ggcctacgcc gatccactgg cgcttgccca ccttgaagcc gaagaagcgc tcggccagca
     6841 cggtcaggaa cacgagcccg cccgagatca ccgactggac gagcgaaagc ggcgccatgg
     6901 ccatcgcgcc cacgtggagc agccatgcgc caaccgccac gaacatgccg atggcgaacc
     6961 acttggacga ccagaggcct accgcgctcc tcagaggatg acgccagtcg acgtcgggtg
     7021 cggcgcacgc cccccggtgc ttatagagaa agcccaggtt ggtggccagc gcgcaaaaca
     7081 gcgcgagcag aatgcctagt tcgagagtca tccagagttg aggctcaagc ggccccgctg
     7141 ccgctattcg aggtatcgac tccagagcct tcagacttga gcccggagcg cctcaggcgg
     7201 actaccttag cggcttgtcg ccttctatgt tggtttttgc caggtcccat ccgcctcaaa
     7261 cccctcaacc gtacgagcat ttgcacccaa ttctgtttct cgatgttgat ggcgtgataa
     7321 gtcttttcgg cttccccgcc gatggccctc caccgggtcg gttccactgg gtcgacggcg
     7381 tggcccactg catcgacgag gcctgcggag agcgcatcgg acgcctctgg gacgcctacg
     7441 aagtcgtctg ggcgaccggc tgggaggaga aggcgaatga gtacctcccc cacatcctgt
     7501 cgctgccggc aagggagctg cccgtggtga gcttcgaaga ccgcgcaccg cttgcgtcgg
     7561 ctcactggaa gctcgaggcg atcgccgagt acgccggtga gcggccggcc gcctgggtgg
     7621 acgacagcct cgatgcggag tgcgaacgct gggcgcgcga acggcgcgcg cccaccctgc
     7681 tcgtccacac gcgcccggag atcggactcc gggagcagga cgtggacgtg ctgctcacgt
     7741 ggggggacga
//
LOCUS       DASVXS010000171         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_540376, whole genome shotgun sequence.
ACCESSION   DASVXS010000171 DASVXS010000000
VERSION     DASVXS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540376"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VF752_09580"
     CDS             <1..821
                     /locus_tag="VF752_09580"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX8855841.1"
                     /translation="AEIARAFIVSEPAMAQRLVRAKRKIAEARISYRVPPDEELPNRL
                     AGVLAVIYLIFNEGWTASGGDRLVRGELCSEAIRLGRVLHQLMPDDPEVAGLLALMLL
                     HDSRREARVDEHGEPVLLDEQDRTLWDMGRIREGWRLLEGALRRKHPGPYQLQAAIAT
                     LHATSMSPAQTDYAGIAELYRELAVFLPSPVIEVNRAVAVGRSAGPEAGLEVLEPVLA
                     SGELDDYAPLHAAHGDLLERLGDAAGAAEAYRQASAVADNAATRRALERRAAAL"
     gene            complement(818..1225)
                     /locus_tag="VF752_09585"
     CDS             complement(818..1225)
                     /locus_tag="VF752_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855842.1"
                     /translation="MDGQRKIEQELDRLDRERRKLTDDWLVVAEARDAARVLHQQTEH
                     ERDSALDARESLADERDRVADERERLADERERLADARERLADTGERLPATLDEGPPTQ
                     RREEHALREQAAIDRQEAATRRDVAAHMSRREG"
     gene            complement(1230..2573)
                     /locus_tag="VF752_09590"
     CDS             complement(1230..2573)
                     /locus_tag="VF752_09590"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX8855843.1"
                     /translation="MTTETDQARELWGGETRKAVDNFSVSGERIPLSVVHWLGRIKAE
                     AARVNAELDLLDAEIAERIAKAGDEVAHGRHDDQFPIDVFQTGSGTSSNMNANEVIAT
                     LAGDGAHPNDHVNMGQSSNDVFPSAVHLAALAESTDKLIPALEVLHDSLARKADEFKD
                     MVKAGRTHLMDAVPVTLGQEFGGYAAQVRLGRQRVADTLGRVGQIPLGGTATGTGLNT
                     HPEFAARVRAKLSQATGLRIEAPEDPFEAQGNRDALVELSGALKVVAVSLTKIANDLA
                     WMGSGPRTGLSEIALPELQKGSSIMPGKVNPIIPEVVLQVCAQVIGNDTAITIGGMQG
                     NFELNVRIPLIARNLLQSIHLLSSAATLLATKVVDGVEANEEITTRHAESTPAIATAL
                     NPYIGYDKATEIVKDAVASRRTIREVAIEKGVDEETLDKALDLRRMARGNSGNSG"
     gene            complement(2597..>2978)
                     /locus_tag="VF752_09595"
     CDS             complement(2597..>2978)
                     /locus_tag="VF752_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855844.1"
                     /translation="DHSLPAACREGSGAVTRALGAAPGRVELADGVPLSRCVQRAVDP
                     DDVVFVGGTFVSVAVGLAERARADIESPAALQLGYLLGAAHRGGDRTLVSSELLRRLD
                     QEAAGIAERSHAYERGLRAGRAAG"
BASE COUNT          393 a         1064 c         1031 g          490 t
ORIGIN      
        1 cggctgagat cgcccgcgcg ttcatcgtgt ccgagcccgc gatggcgcag cggctcgtgc
       61 gcgcgaagcg caagatcgcc gaggcccgga tctcctatcg cgtgccgccg gacgaggagc
      121 tgccgaaccg cctcgccggc gtgctcgccg tgatctacct gatcttcaac gagggctgga
      181 ccgcgagtgg cggcgaccgg ctcgtgcgcg gcgagctgtg ctcagaggcc atccggctcg
      241 ggcgggtgct tcatcagctg atgccggacg atcccgaggt ggccggcctg ctcgcgctca
      301 tgctccttca cgactcgcgc cgcgaggcgc gcgtggacga gcacggcgaa ccggtgctgc
      361 tggacgagca ggaccgcacg ctgtgggaca tgggccgcat tcgcgagggc tggcggctgc
      421 tcgagggcgc cctgcggcgg aagcaccctg gcccgtacca gctgcaggcg gcgatagcca
      481 cgctgcacgc gacctcgatg agtccggcgc agaccgacta cgccgggatc gcggagctct
      541 accgcgagct cgcggtgttc ctgccctccc ccgtgatcga ggtgaaccgc gcggtggccg
      601 tcggccgtag cgcgggccct gaggcgggac tcgaggtgct cgaaccggtg ctggcctcgg
      661 gcgagctcga tgactacgcg ccgctccacg cggcgcacgg tgacctgctc gagcgactgg
      721 gtgacgccgc gggcgcggcg gaggcctacc gccaagcttc ggcggtcgcg gacaacgccg
      781 cgacgcgtcg agcgctcgag cgccgcgcgg ccgccctcta gccctctctt ctcgacatgt
      841 gtgcggcgac gtcccgccgc gttgccgctt cctgtcggtc gatcgcggcc tgttcccgca
      901 gcgcgtgctc ttcgcggcgc tgcgtcggcg ggccctcgtc cagcgtggcg gggaggcgct
      961 cgcccgtgtc cgcgaggcgc tcccgggcgt cggccaggcg ctctcgctcg tcggccaagc
     1021 gttctcgctc gtcggcgacg cggtcacgct cgtcggcgag cgactcgcga gcatcgagcg
     1081 cgctgtcgcg ctcatgctcg gtttgctggt gcagcactcg ggccgcgtcc cgggcctccg
     1141 caacgaccag ccagtcgtcc gtcagcttgc gccgctcgcg atcgaggcga tccagctctt
     1201 gctcaatttt cctctgcccg tccacgtcat cacccactgt tcccactgtt ccccctcgcc
     1261 atcctgcgca ggtcgagcgc cttgtcgagc gtctcctcat ccacgccctt ctcgatggcg
     1321 acctcacgga tggtacgcct cgacgcgacc gcgtccttca ctatctcggt cgccttgtcg
     1381 tacccgatgt aggggttgag agcggtcgca atcgccggtg tcgactcagc atgccgcgtc
     1441 gtgatctctt cgttggcctc caccccgtcg accaccttgg tggcgagcag cgtggcagcg
     1501 gagctgagca ggtggatcga ctggagcagg tttcgcgcga tcagcggaat gcgcacgttc
     1561 agctcgaagt tgccctgcat gccgccgatc gtgatggcgg tgtcgttgcc gatcacttgg
     1621 gcgcacacct ggagcaccac ctcggggatg atcggattga ccttgcccgg catgatcgac
     1681 gaccccttct gcagctcagg cagcgcgatc tcgctcagcc cggtgcgcgg gccggagccc
     1741 atccaagcga ggtcgttcgc gatcttggtg agcgacacgg cgaccacctt cagcgcgccc
     1801 gagagctcaa caagcgcgtc gcggttgccc tgcgcctcga agggatcctc tggcgcctcg
     1861 atgcgcaggc ccgtggcctg gctcagcttc gcgcgcacgc gcgcggcgaa ctcggggtgg
     1921 gtgttgagtc ccgtgccggt ggccgtaccg ccgagtggaa tctgacccac gcgcccgagc
     1981 gtgtccgcca cgcgctggcg gccgaggcgt acctgcgccg cgtagccgcc gaactcctga
     2041 ccgagcgtga cgggcaccgc gtccatcagg tgcgtgcggc ccgccttcac catgtccttg
     2101 aactcgtcgg cctttcgcgc cagcgagtcg tgcagcactt ccagggcagg gatcagcttg
     2161 tcggtggact cggcgagcgc ggcgaggtga actgcggagg gaaacacgtc gttcgacgac
     2221 tgccccatgt tcacgtggtc gttcggatgc gcgccgtcgc ccgcgagagt cgcgatcacc
     2281 tcgttggcgt tcatgttcga agaggtgccc gagccggtct ggaagacgtc gatcgggaac
     2341 tggtcgtcgt ggcggccgtg agccacctcg tcgccggcct tcgcgattcg ctccgcgatc
     2401 tctgcatcga gcaggtcgag ctcggcgttc acgcgggcgg cctcggcctt gatccgtccg
     2461 agccaatgga ccaccgacag cgggatccgc tcgcccgaga cggagaagtt gtcgacggcc
     2521 ttgcgcgtct ctccgcccca gagctcgcgc gcctgatcag tctcagtcgt catgaacctg
     2581 aggctatcgc tcgccctcag ccggcggctc gcccggcgcg cagcccgcgc tcgtacgcgt
     2641 gcgagcgctc ggcgattccg gcggcctcct gatcgaggcg gcggagcaat tcgcttgaga
     2701 cgagcgtgcg gtcgccaccg cggtgcgccg cgccgagaag gtagccgagc tgaagcgcag
     2761 cgggcgactc gatgtccgcc cgcgcgcgtt cggcgaggcc gacggccacg gacacgaagg
     2821 tgccgccgac gaagaccacg tcgtcaggat ccacggcccg ctggacgcac cgcgacagcg
     2881 ggacgccgtc cgcaagttcc acgcgtcccg gcgcggcgcc gagtgcgcgc gtgacggcgc
     2941 cgctgccctc acggcaggcg gccggcaggc tgtgatcg
//
LOCUS       DASVXS010000172         6122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_328618, whole genome shotgun sequence.
ACCESSION   DASVXS010000172 DASVXS010000000
VERSION     DASVXS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328618"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VF752_09600"
     CDS             complement(<1..1122)
                     /locus_tag="VF752_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX8855845.1"
                     /translation="MEALQASGRRIFFFGALGGLLFGYDTGVISGAILFIKKDFGLSP
                     FMQGAVVAALLLGAMIGAGLAGPLSDRLGRRRLIMTAAVTFTVGALWAAAAPNPGNLL
                     AARFVLGLAVGSAALVVPLYLSEIAPTHIRGAIASLNQLMIVIGILAAFIVNAILASS
                     GDWRLMLGLAAVPSVILFIGMFFMPETPRFLVKTGEEGEARDVLEEVHEDGHKDDHPD
                     RKIEEIREADEREEEGGGVRMLLKQRWVRPALIVAIGLAVFQQFIGINTIIYYAPTTL
                     TSVGFGPESAIYLNLIIGVLNVAMTVVAIRLIDRVGRKPMLLAGLVGMVTSLLVLGLS
                     SELLAKPSAATDPAAIITLICLAGFIVSFAATWGPVVWVM"
     gene            1253..1639
                     /locus_tag="VF752_09605"
     CDS             1253..1639
                     /locus_tag="VF752_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855846.1"
                     /translation="MGAENVALVREIFEAWQAEDFDALERGFSPNVQWVGLKPEEGDC
                     HGAAEVAQVLRDRSAQGVRVELEELIDVGDQVVAVVRTSGRPDLPPGERIIFITVTLR
                     GGTVAAMQSFHSRDEALSAGPALDLR"
     gene            complement(1641..2603)
                     /locus_tag="VF752_09610"
     CDS             complement(1641..2603)
                     /locus_tag="VF752_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855847.1"
                     /translation="MSRRLVVSSAVIVIALAAVALGRLASEGEATTPGTNGLIAYAKE
                     VGGHFQLFTIRPDGTGERQITHVDGDAVHPDWSPDGNRIAFEFDRPRGCSVMLVNADG
                     SGLADLTGTRNGCENQPAFMPDGQRLVFVRFDDRTEVESIQSMNLSGGDLRLITGRKG
                     AGRTDPNVSPDGKTITFVRLKKEHVLQALFSVHADGSGLKRVTPYRWEVAIKHDWAPN
                     GKRIALTTNADFVRPNASANLVTIRPDGSGLKRLTHFTGGPVKGKNAFAGSYSPDGRQ
                     IVVRIEQRDRGGLAVIGADGRHLHMLTRASKNKPRFIDWGTHAP"
     gene            2930..3205
                     /locus_tag="VF752_09615"
     CDS             2930..3205
                     /locus_tag="VF752_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855848.1"
                     /translation="MALGTVAGGAALGLAGRAAFKRGRRPRMLGGRPNLNQQLGELAK
                     QVGRAGASAGALAIELRQLREQADERKRQSPIEVVLSALTSRGLPRH"
     gene            complement(3222..3292)
                     /locus_tag="VF752_09620"
     tRNA            complement(3222..3292)
                     /locus_tag="VF752_09620"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3258..3260),aa:Cys,seq:gca)
     gene            3348..4307
                     /locus_tag="VF752_09625"
     CDS             3348..4307
                     /locus_tag="VF752_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX8855849.1"
                     /translation="MRDRIQISPELYRKIAVTALGALVLIVLTGAAVRLTGSGLGCPD
                     WPRCYGRTVAPLESHAVIEYTNRLLSGFVGVAVIAAGVLAWFRRPFRWHLALFGGLLP
                     IGVIAQAILGALVVKYHLAPGLVMSHFILSMMLLDAAFALAWCATYEPGERRASTDRL
                     GVWAVRALIPIGQLTILVGTITTAAGPHAGAHGEELVHRFTFKGGNTLSWMVERHSVI
                     AGLFGISAIAVWLLLHREGGDRRALRPLTWVIGLLALQGVVGFTQYQLKLPSELVWVH
                     VALATLTWVAMLWTVATAGRLAPRLEPVPGAVAERSGEPAGVA"
     gene            complement(4312..4911)
                     /locus_tag="VF752_09630"
     CDS             complement(4312..4911)
                     /locus_tag="VF752_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8855850.1"
                     /translation="MSKPWHPYVFDEKNRAFVGDFESMYNAEQELGFDSWHQSDPRRL
                     DTRVASMLLEEITYSRAVDLGCGKGSFTAQLKRRDNQVIGVDSSKTAIRLAASYFPDI
                     EWVCSPVDEYLGSAPPVDLIVARELLSYLQGWRDVVTSCALLTRYFLVSLYLPPDPIG
                     YVKSHSDLEDELARHFEIVEAIVLKRRSLAVHLCESLTR"
     gene            complement(4911..>6122)
                     /locus_tag="VF752_09635"
     CDS             complement(4911..>6122)
                     /locus_tag="VF752_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855851.1"
                     /translation="AALVLPALAGAGAAALTAAIAGKMGASRRGAVGAACAFALGTQA
                     LTYSRTLFAEVLVAFLLALVVWGITDRSRGRRQLGYVALGLAVLAKPQTIVVAPGLAI
                     ALVLAYRSWRPVIGIAAASISGSLVYLAYNVARFGDPLTFGARDRMLQADWFRPGRLL
                     DGLAVLIVSPGRGLVWFSPVAIIGALVLWRRRQSPVAAAALGVSLPILVLSIAHPAGW
                     QAWGARYLIPTLPLVCAGIGLLSGRKATLAAALVAIGFVNQIPTTVAFTDRYFTEARE
                     QHATTHELRWERFAVADMWATAARQVRDALHTDVRHVVANAGGQRSETVAGQPVLRVV
                     SLWWWVLPAVRVSRWIGAIACLLGLLAAGRLLWRASEDTVRARAGPAVERGGASREGA
                     RWVRAAENEVR"
BASE COUNT         1026 a         2128 c         2043 g          925 t
ORIGIN      
        1 catcacccac acgaccgggc cccacgtcgc agcgaacgag acgatgaagc cggcgaggca
       61 gatcagcgtg atgatcgccg ccgggtcagt cgccgcgctc ggcttcgcca gcagctctga
      121 cgagagaccg agcaccaaga ggctcgtgac catgccgacg aggccggcca gcagcatggg
      181 cttgcggccc acgcggtcga tgagccggat cgcgaccacc gtcatcgcga cgttcagcac
      241 gccgatgatg aggttcaggt agatcgccga ctccggtccg aaccccacgc tcgtgagcgt
      301 ggtcggcgcg tagtagatga tcgtgttgat tccgatgaac tgctggaaca ccgcgagccc
      361 gatcgcgacg atcaacgccg ggcgcaccca gcgctgcttg agcagcatgc gtacgccgcc
      421 gccctcctcc tcgcgctcgt cggcctcgcg gatctcctcg atcttgcgat ccggatggtc
      481 atccttgtga ccgtcctcgt ggacctcctc gagcacgtcg cgggcctcgc cctcttcccc
      541 ggtcttgacg aggaaccgcg gcgtctcagg catgaagaac atgccgatga agaggatcac
      601 cgacggaacc gccgccagtc cgagcatcag ccgccaatcg ccggacgacg ccaggatcgc
      661 gttcacgatg aacgcggcca ggatgccgat cacgatcatc agctggttga gcgatgcgat
      721 cgccccgcgg atgtgcgtcg gtgcgatctc agacaggtac agcggcacca ccagcgccgc
      781 agaaccgacc gccaggccaa gcacgaaccg tgccgcgagc aggttcccag ggttcggtgc
      841 ggccgctgcc cagagcgcgc cgaccgtaaa ggtgaccgcg gcggtcatga tcagccgccg
      901 tctccccagc cggtctgaca ggggaccggc taacccggcc ccgatcatcg cgcccagcag
      961 cagcgcggcg accaccgcac cctgcatgaa cggcgacaga ccgaagtcct tcttgatgaa
     1021 caagatcgcg ccggagatga cgcccgtgtc gtagccgaac agcagcccgc cgagggcgcc
     1081 gaagaagaag attctccggc cgctggcctg tagtgcttcc atgtaactcg cctccctacg
     1141 cccctttagc tcccatattt ggagtgcccc aggcaaggag gcgcaaactg gcaggcacgc
     1201 gccagttcag gccccgctgg acagtgctct gggcgcgcgc aagactccgg gcatgggcgc
     1261 ggagaacgtc gctctggtgc gggaaatctt cgaggcctgg caggccgaag atttcgacgc
     1321 gctcgagcgc gggttctcac caaacgttca gtgggtgggc ctcaagcccg aagagggcga
     1381 ctgccatggc gcggctgagg tggctcaggt gctccgcgac cgcagcgcgc agggcgtccg
     1441 tgtggagctc gaagagttga tcgacgtcgg cgatcaggtg gtggcagtcg ttcgcacttc
     1501 cggccggccc gacctcccgc ccggcgagcg gatcatcttc atcacggtca cactccgcgg
     1561 cggcaccgtg gcggcgatgc agagcttcca cagccgcgac gaggcgctgt cggccggtcc
     1621 cgcgctcgac ctgcgctagc tcacggggcg tgcgtgcccc agtcgatgaa cctcggcttg
     1681 ttcttgctcg ccctggtgag catgtgcagg tgcctgccgt cggcgccgat gacggccaag
     1741 ccgcccctgt cgcgctgctc gatccgaacc acgatctgcc ggccgtcggg ggagtacgac
     1801 cccgcgaagg cgttcttccc cttcaccggc ccgcccgtga agtgcgtcag ccgcttcagc
     1861 cccgagccgt cggggcggat cgtcacgagg ttcgcggaag cgttgggccg cacgaagtcg
     1921 gcgttcgtcg tgagcgcgat gcgcttgccg ttcggtgccc aatcgtgctt gatcgcgacc
     1981 tcccagcggt aaggcgtcac ccgcttgagc cccgagccgt ccgcgtggac ggagaacaac
     2041 gcctgcagca cgtgttcctt cttgagccgg acgaatgtga tcgtcttgcc gtcaggcgac
     2101 acgttcggat cggtgcgacc cgctcccttt ctcccggtga tcagacggag gtcgccgccc
     2161 gacaggttca tgctctggat cgattcgacc tcggtcctgt cgtcgaagcg cacgaataca
     2221 agcctctgcc cgtctggcat gaacgcgggc tggttctcgc agccgttcct cgtgccagtg
     2281 agatccgcga gcccgctccc gtcggcgttg acgagcatca ccgagcagcc gcgcgggcga
     2341 tcgaactcga acgcgatgcg gtttccgtca ggcgaccagt cggggtggac ggcgtccccg
     2401 tcgacgtgcg tgatctggcg ctccccggtt ccgtcggggc ggatcgtgaa cagctggaag
     2461 tgcccgccca cctccttcgc gtacgcgatc aggccgttcg tccccggggt cgttgcctct
     2521 ccttccgagg cgagacgacc gagcgccaca gcagcgagtg cgatgacgat cacagcgctc
     2581 gagacgacaa ggcgcctgga catgaatcca gcccttcgcg gtcgaagcct gtctagccga
     2641 ccctaacaga ggcgccgacc aggaatcgaa ccggtgtagg gattcggccc gcgctttgca
     2701 ggcgttttct ggttctctca tcgcgggtac gacctcttcg atggcgacca acaggaacac
     2761 gaccagcgca acgcggccga gaccggcggg gcgccgctcg agagcctcta acgcgagcgc
     2821 tcggagtcgc agccgggcga gaactcagag gaagagccag aacgggcggg tacagacggt
     2881 cgagtccgcc gccaccaagg cgtggagcgg taccggcacg gccggccggg tggcactggg
     2941 cacggttgcg ggtggcgcag cgctcggcct cgcggggcgc gccgcgttca agcgtgggcg
     3001 ccggccacgc atgctcggcg gacgcccgaa cctgaatcag caactcggag agctcgcgaa
     3061 gcaggtcggt cgcgcgggtg cgagcgccgg agcactggcg atcgagctgc ggcagctccg
     3121 tgagcaggcc gacgagcgca agcggcagtc gccgatcgag gtggtgctga gcgcgctcac
     3181 ttcgcgcggc ctgcctcggc actgaggaac cacgccagta gaggcgccga ccggaatcga
     3241 accggtgtaa acggctttgc aggccgctgc gtaaccactc cgccacggcg ccggagcccg
     3301 cctagcgtag cgacccagac ggcgctcgga cggctacatt gggggccgtg cgggaccgca
     3361 ttcagatcag cccggagctc taccggaaga tagcggtgac ggcgctgggc gctctcgtgc
     3421 tcatcgtcct cacgggcgcc gcggtgaggc tcaccggctc gggcctaggc tgcccggact
     3481 ggccgcgctg ctacgggcgc acggtcgccc ctctcgagtc gcatgcggtg atcgagtaca
     3541 cgaaccggct gctgtcgggc ttcgtcggcg tcgccgttat cgcggcgggc gtgctcgctt
     3601 ggttccgccg gccattccgc tggcacctgg cgctgttcgg cggcctcctg ccgatcggcg
     3661 tgatcgcgca ggcgatcctc ggggcgctcg tcgtgaagta ccacctcgcg ccgggcctcg
     3721 tgatgagcca cttcatcctc tcgatgatgc ttctcgacgc ggccttcgcg cttgcctggt
     3781 gtgcgaccta cgagccgggc gagcggcgcg cctccaccga ccgcctggga gtctgggccg
     3841 tgcgggcgct catcccgatc ggccagctca cgatcctcgt gggcacgatc accaccgctg
     3901 cgggcccaca cgccggcgcc cacggcgaag agctcgtgca ccgcttcacg ttcaagggcg
     3961 gcaacacgct cagctggatg gtggagcgcc actcggtgat cgcgggcctg ttcggcatct
     4021 ccgcgatcgc ggtctggctg ctgctccacc gcgaaggcgg cgaccgccgc gcgctgcgcc
     4081 cactcacgtg ggtgatcggg ctgctcgctc tgcagggcgt cgtcggcttc acccagtacc
     4141 agctgaagct gccctcggag ctcgtgtggg tgcacgtggc gctggcgacg ctcacgtggg
     4201 tcgcgatgct gtggaccgtc gcgaccgcgg gacgtctcgc gccgcgcctc gagcctgttc
     4261 cgggcgcggt cgcggagcgg agcggagagc cggccggcgt ggcctgacgc gctatcgggt
     4321 cagcgactca cagaggtgta cggccagaga acgccgtttc aacacgatcg cctcaacgat
     4381 ctcgaaatgc ctcgcgagct cgtcctccag atcggagtgc gatttgacgt aaccgatggg
     4441 atccggcggc aggtagaggc tgacgaggaa atagcgcgtg agaagagcgc aggatgtcac
     4501 cacgtcgcgc cagccctgga ggtagctgag cagctcccgc gcgacgatca ggtccaccgg
     4561 cggcgccgac cccaggtact cgtcgaccgg cgagcagacc cactcgatgt cggggaaata
     4621 cgaggccgcc aagcggatgg cggtcttcga ggagtcaacc ccgatcacct ggttgtcccg
     4681 gcgcttgagc tgcgcggtga aggacccttt cccgcagccg agatccaccg cgcgggaata
     4741 ggtgatctcc tcgagcagca tcgaggcgac ccgagtatcg agccggcgcg ggtcggattg
     4801 atgccaggaa tcgaaaccga gttcctgctc ggcgttgtac atcgattcga agtcgccgac
     4861 gaaggcgcgg ttcttctcat cgaatacgta tgggtgccag ggtttcgaca tcaccggact
     4921 tcgttctcgg ctgcccgcac ccagcgagcg ccttcgcgcg acgcaccccc gcgctccacg
     4981 gccggaccgg cgcgcgcacg taccgtgtct tcggaggcac gccacagaag ccgtccggct
     5041 gccagcagcc cgaggaggca ggcgatcgct ccgatccagc gtgagacacg aaccgccggc
     5101 agcacccacc accacagcga cacgacacgc aggaccggct gacccgcgac cgtctccgat
     5161 cgctgcccac cggcgttcgc aactacgtgt cgcacgtccg tatgaagcgc atcacgaacc
     5221 tgacgagctg ccgtcgccca catgtctgcg acagcgaagc gttcccacct gagctcgtga
     5281 gtcgtggcgt gctgctcacg agcctcggtg aaatagcggt cggtgaaagc gaccgtcgtc
     5341 ggaatctggt tcacgaatcc gatcgccacg agggccgccg ccagcgtcgc tttgcgtccg
     5401 ctcaggagac cgatgcccgc gcacacgagc ggcagcgtcg gaatgagata tcgcgcgccc
     5461 catgcctgcc accctgcggg gtgcgcgatc gataggacga ggatgggcag cgacacccca
     5521 agcgccgccg cggcgaccgg cgactgtcgc ctccgccaga gaacaagcgc gccgatgatc
     5581 gcgaccggcg aaaaccacac caggcctctc cccggactga cgatcagaac ggcgaggccg
     5641 tccagcagtc gcccgggcct gaaccagtcc gcttggagca tccggtctct cgccccgaac
     5701 gtgagcggat cgccgaacct cgcaacgttg taggcgaggt agacgagtga gccgctgata
     5761 ctcgcggccg caatcccgat caccggccgc cagctccggt acgcaaggac gagcgcgatg
     5821 gcgagcccgg gcgcgacgac gatcgtctgc ggcttcgcga gcaccgcgag cccgagcgcg
     5881 acgtatccga gctgtcggcg cccgcgcgat ctgtccgtga taccccacac gacaagggcg
     5941 agcaggaatg ccaccagcac ctcggcgaac agcgtccgcg aatacgtgag cgcctgggtc
     6001 ccgagggcga acgcacacgc cgcgccaacc gcccctcgac ggctggcgcc catcttcccg
     6061 gcaatcgccg ccgtgagcgc cgccgcgccg gctccggcca gcgcggggag cacgagcgcg
     6121 gc
//
LOCUS       DASVXS010000173         9837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_541467, whole genome shotgun sequence.
ACCESSION   DASVXS010000173 DASVXS010000000
VERSION     DASVXS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541467"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VF752_09640"
     CDS             complement(<1..681)
                     /locus_tag="VF752_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8855852.1"
                     /translation="MPTATDLFEKARNHERLEQLKAAREHDLLPYFRLIEGEAGPVME
                     MEGRERIMLGSNNYLGLTGDERVKNAARQALEEYGTGLTGSRFLNGTTPLHLELEREL
                     AEWMGTEDALSFTTGYQANLGTIGTILQPGDTVIVDSGDHASIMDGVALARARIRPFR
                     HNRLDKLEQMLERGAEDGGGVLVVVDGVFSMEGDVCDLAPIVELCKRFGARLMVDEAH
                     GVGVLGARG"
     gene            722..1858
                     /locus_tag="VF752_09645"
     CDS             722..1858
                     /locus_tag="VF752_09645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8855853.1"
                     /translation="MSVDVRPVKTKSELKSFVKLPWRLYRNEPLWVPPLIHDRMKFLD
                     RSKNPFFEHAEAEYFLAYRNGEPVGRITAHVDHLLNEVHGTEWGQFGFFETEDDPQVA
                     KALLDTAEAWLRERGRDRMVGPMDFSTNHECGLLVDGFERPPQILELWHHPYYGKLLE
                     EWGLAKAMDLYKWQLYIQDREKVHPIIWQLAEKLEPEHGIRIRHMSKRDFKNEVKRFM
                     DVYNSAWEKNWAFVPLTDAELVQYAKDLRPVLDPNWAMMAENGDGEIVGCALTLPDYN
                     QVLKHLDGRLLPLGWLKALRKRKEINTVRVFALGVKPEYQHTGVAAGLYAEHFAMAAA
                     TPQTGGEMGWILESNTAMNKAMEGMGGKIVKTYRIYEKTFRPGG"
     gene            1871..2179
                     /locus_tag="VF752_09650"
     CDS             1871..2179
                     /locus_tag="VF752_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855854.1"
                     /translation="MSEQAENSENGRHLPVLAEPAEARPIERPAEQEQLPVVLGMPAP
                     LAAATGGFLAGVATFLLVRVLRGGRRASLPARRSRRRGRSIEIAGTRSFLVDVHLIKR
                     "
     gene            2180..3031
                     /locus_tag="VF752_09655"
     CDS             2180..3031
                     /locus_tag="VF752_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855855.1"
                     /translation="MVALERDVVPCGAYRMPRAGRDGVLRRRKHDRALVRLIHHGDEP
                     AVVSARPCGGAVRIHAEAETRLAAAFAIDRMRFALGVDHDLRPFHRRFRADPLIGPVI
                     RRRPWLRPGRIADPFQALAWAITEQLIEVERAEAIQRRLVWRYGRVSNCGSWRDAPSA
                     AQLAARAPAELESCDLAGKRAIAMVRVGREVASGRADLEHHEPTWQRLLKIREIGHWT
                     VEKLAFFGQGRDDMLPAGDLAYIKAVGRLAGLGRRATVDEVREFFAPYAPYEGLAGVY
                     ALSRARG"
     gene            complement(3591..5519)
                     /gene="nhaA"
                     /locus_tag="VF752_09660"
     CDS             complement(3591..5519)
                     /gene="nhaA"
                     /locus_tag="VF752_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX8855856.1"
                     /translation="MAVDATAEATPPAARRFSEKTAWARNLTAPLRDYLNTETGGALA
                     LLAAAVAALVWANSPWFHSYESVWTTTLSIRIGDSGISQDLRHWVNNGLMTLFFLVVG
                     LEAKRELDVGQLRERRRVGIPLVAALGGMALPIAIYLAFNAGGAGAKGWGAAMSTDTA
                     FALGVLALLAPAGTRLRVRLLTVAVFDDLVALIVIATVYTKHVNVTSLLAGVVIFGLL
                     LASRHAPQAIRGPIAAVLGVALWVALYKSGIDPVISGLLIGLGTTAYTPERGRLERVV
                     ELTRLFREQPTPELARSAQRGVASAISANERLQYELHPWTSFVIVPLFALANAGIHIN
                     GDLLGRAYTSPITLGIIFGYVLGKPLGILGATWIATRLGLRRSLSWPAITALGAVAGI
                     GFTVSLLISTLAFHGDTLEEAKIGVLTAAVLAALISWGVVSLYKRTPTEVRARRIAGT
                     LDELVDLAEDIDPERDHIRGDPDAPVTLVEYGDYQCPYCGQAEVVVRELLNSFGDELR
                     YVWRHLPLNDVHANAQMAAEASEAAAAQGAFWEMNDKLLAHQDELGPKDLGRYAEEIG
                     LDLDKFWDDIRHHRHAERISDDVASADASGVAGTPSFFINGTRHQGAYDVDTLTRAVR
                     AAQVRADAMRANQPAAAG"
     gene            complement(5553..5930)
                     /locus_tag="VF752_09665"
     CDS             complement(5553..5930)
                     /locus_tag="VF752_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855857.1"
                     /translation="MQTEAFKVWRNQPDETAPVEDPWLGQLFDAIDSHIGSMAEHLER
                     VLVEELRSAAAVAVADLDDRPVVNERHRSLAHFAFGESEHAERFLKRAGQEDIDLLEA
                     WYALSTARPPGFMARARRLLKSG"
     gene            complement(5954..6970)
                     /locus_tag="VF752_09670"
     CDS             complement(5954..6970)
                     /locus_tag="VF752_09670"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8855858.1"
                     /translation="MRRRVSAFAVALATLALCVGAAGCGEKKEPLKPGATVPFELMLD
                     FFPNADHAGIYEAKAQGLFKSLGLDVKIRTPSDPAAPLKQVAAGRVDLAISYEPELLR
                     ARDQGLHVVAVGALVRTPLTSIISLPKARIDKPADLAGKTVGTAGIDYQHAYLEAILK
                     NANVPPSRVKERNVGFNLVPALVSKKVDSILGGYWNYEGIQLAQQKRHPKIIRIEKAG
                     VPTYDELVLVANADGLEQNGAKIRSFIGALARGTRELNRNPEQGIKALLAANPDLDAK
                     LQRASVKVTLPFMLPPKDKPFGYMNGKEWDSFAEWMSESGILNQVSSAAGAFTSKYLP
                     GAGI"
     gene            complement(6967..7719)
                     /locus_tag="VF752_09675"
     CDS             complement(6967..7719)
                     /locus_tag="VF752_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855859.1"
                     /translation="MRRWWLSVLLFAAFIGLWQLVASLPGVDNLTLASPAETLRAIRH
                     DHALLVDNMWVTLEEVLLGLAIAVLAGAGSAILMHLFRPLREAAYPLLVASQAIPIVV
                     LAPLFVLAFDYGIGPKLAIVALICFFPITVNVLDGLRSVDPELLKLMRSLRASRVATL
                     VRVELPSSLPFFFSGLRVAATVSVIGAVFGEWAGADKGLGRLVLLGNNQLQTPRVYAG
                     VVILTLMAVALFALVTLVERVAVPWKRGGSPA"
     gene            complement(7716..8420)
                     /locus_tag="VF752_09680"
     CDS             complement(7716..8420)
                     /locus_tag="VF752_09680"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855860.1"
                     /translation="MSALEAVDLRVREGEVLAVVGPSGCGKSTLLELIAGLQEPDAGT
                     LSALGHAAAADRLAACAYMPQRDLLLPWRDGLGNAALALEVQGVSRKEARRRAAPLFE
                     RFGLAEFEQSPPSELSGGMRQRVAFLRTLLSGRPVLLLDEPFAALDSLTRGAMQEWLA
                     EALAHEPRTVVLVTHDVEEAIYLGDRVAVLSPRPGRVVAEIAVPLARPRGRRRDAIAA
                     PAFAELRHRALEALEG"
     regulatory      complement(8512..8633)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8684..9019
                     /locus_tag="VF752_09685"
     CDS             8684..9019
                     /locus_tag="VF752_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855861.1"
                     /translation="MAGELVYFVIPTRQGERAKRFYGELFGWRFGEGNVPGGSQIENT
                     NPAGGMFENPEGTVPQVFFQVDDIHAAVERVRELGGEGAEPQRVESGWMAACRDDQGV
                     ELNLWAPAS"
     gene            9092..9565
                     /locus_tag="VF752_09690"
     CDS             9092..9565
                     /locus_tag="VF752_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8855862.1"
                     /translation="MTASTVVREQGQGEAYWMLGGLYEVKVSGAESDGELTLMQMTIP
                     AGMGPPPHTHPGGETVCVLDGRIRYHIGGETHEGGPGSCFHVPAGIEENFEPLEETRL
                     LVAYTPGGVDRFFAEAGEPAARRVVPPPPEGPPDMARLMEISERYGVHIHAPVET"
     gene            complement(9562..>9837)
                     /locus_tag="VF752_09695"
     CDS             complement(9562..>9837)
                     /locus_tag="VF752_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855863.1"
                     /translation="KKLDGRYVSVSGTVVANERAEAPGVDVRRELQLKDPASDALAYC
                     ATADEPDAPVKSRARVTGLIVAYGPVRTDGGRSAPAVYMRCFDVKRF"
BASE COUNT         1533 a         3338 c         3475 g         1491 t
ORIGIN      
        1 gccgcgcgcg ccgagcacgc cgacgccgtg tgcctcgtcc accatcaggc gggcgccgaa
       61 gcgcttgcag agctccacga tcggcgcgag gtcgcacacg tcgccctcca tcgagaacac
      121 gccgtcgacc accacgagca cgcctccgcc gtcctcggcc ccgcgctcca gcatctgctc
      181 gagcttgtcg aggcggttgt ggcggaacgg ccggatgcgc gcccgggcga gcgcgacgcc
      241 gtccatgatc gaggcgtggt cgccggagtc gacgatcacc gtgtcgcccg gctgcaggat
      301 cgtcccgatc gtgccgaggt tcgcctggta gccggtggtg aaggagagcg cgtcttcggt
      361 gcccatccac tccgccagct cgcgctcgag ctcgagatgg agcggcgtcg tgccgttgag
      421 aaagcgcgat ccggtgaggc cggtgccgta ctcctcgagc gcctgccggg cggcgttctt
      481 cacgcgctcg tcgccggtca gcccgaggta gttgttcgag ccgagcatga tccgctcgcg
      541 gccctccatc tccatgaccg gccccgcctc accctcgatc aggcggaagt aggggaggag
      601 gtcgtgctcg cgcgcggcct tcagttgctc aagccgctcg tggttgcgcg ccttttcgaa
      661 gagatctgtt gccgtaggca tgctggcttg cgaatggcgg tttgaactaa ggtgccccgc
      721 cgtgtccgtg gacgtccgcc cagtcaagac gaagagcgaa ctgaagagct tcgtgaagct
      781 cccgtggcgg ctttatcgta acgagccgct ctgggtgcca ccgctcatcc acgatcggat
      841 gaagttcctg gacaggtcga agaacccgtt cttcgagcac gcggaggcgg agtacttcct
      901 ggcttaccgc aacggcgagc cggtgggccg catcaccgcg cacgtcgacc acctgctcaa
      961 cgaggtgcac gggaccgagt gggggcagtt cggcttcttc gagaccgagg acgacccgca
     1021 ggtggcgaag gcgctgctcg acaccgccga ggcctggctg cgcgagcgcg ggcgagaccg
     1081 gatggtcggg ccgatggact tcagcaccaa ccacgagtgc ggcctgctcg tggacggctt
     1141 cgagcgcccg ccgcagatcc tcgagctgtg gcaccacccc tactacggga agctgctcga
     1201 ggagtggggc ctcgcgaagg cgatggatct ctacaagtgg cagctctaca tccaggaccg
     1261 ggagaaggtg cacccgatca tctggcagct cgccgaaaag ctcgagcctg agcacgggat
     1321 ccggatccgg cacatgagca agcgcgactt caagaacgag gtgaagcgct tcatggacgt
     1381 ctacaactcg gcgtgggaga agaactgggc gttcgtcccg ctcactgacg cggagctggt
     1441 ccagtacgcg aaggacctgc ggccggtgct cgacccgaac tgggcgatga tggccgagaa
     1501 cggcgacggg gagatcgtgg gctgcgcgct gacgctgccc gactacaacc aggtgctgaa
     1561 gcacctcgac ggcaggctgc tgccgctcgg atggctgaag gcgctgcgca agcgcaagga
     1621 gatcaacacg gtgagggtgt tcgcgctggg cgtgaagccg gagtaccagc acaccggcgt
     1681 ggccgcgggg ctatacgcgg aacacttcgc gatggcggcc gccactcccc agacgggcgg
     1741 cgagatgggc tggatcctcg aaagcaacac cgccatgaac aaggcaatgg agggcatggg
     1801 cggcaagatc gtcaagactt accgcatcta cgagaagacc ttccgaccgg gcgggtagca
     1861 tccgcgactc atgtccgaac aggctgagaa cagcgagaac ggccggcatc tgccggtgct
     1921 ggcggagccg gccgaggcac ggccgatcga gcgccccgcg gagcaggagc agctgccggt
     1981 ggtgttgggc atgccggctc cgctcgccgc ggcgacggga ggcttcctcg cgggagtcgc
     2041 gaccttcctg ctggtgcgcg tgctccgcgg gggccggcgc gcttcgctgc ccgcacggcg
     2101 gtcgaggcgg cgcgggcgct ccatcgagat tgccggcacg cgctcgttcc tcgtcgacgt
     2161 ccacctgatc aagcgctagg tggtcgcgct cgagcgcgac gtggtgccct gcggcgctta
     2221 ccggatgccg cgcgcggggc gcgacggcgt gctgcggcgc cgaaagcacg accgagcgct
     2281 ggtgcggctg atccaccatg gcgatgagcc ggccgtggtg agcgcgcggc cgtgcggggg
     2341 cgcggtgcgg atccacgccg aggccgagac gcgcctcgcg gcggcgtttg cgatcgaccg
     2401 catgcgcttc gcgctcggcg tcgatcacga cctgcggccg ttccaccggc gcttccgcgc
     2461 ggatccgttg atcggtcccg tcatccgccg ccgtccgtgg ctgcgccccg gccggattgc
     2521 tgacccgttc caggcacttg cgtgggcgat caccgagcag ctcatcgagg tcgagcgcgc
     2581 ggaggcgatc cagcggcggc tggtgtggcg ctacgggcgc gtgagcaact gcggcagctg
     2641 gcgcgacgcg ccgtcggccg cacagctcgc ggccagggcg cccgcggagc tcgagagctg
     2701 cgacctcgcc ggcaagcggg cgatcgcgat ggtccgagtg gggcgcgagg tggcgtccgg
     2761 acgcgccgac ctcgagcacc acgagcccac ctggcagcgg ctgctcaaga tccgcgagat
     2821 cggccactgg acggttgaga agctcgcctt cttcggccag ggacgcgacg acatgctgcc
     2881 cgccggcgat ctcgcgtaca tcaaggcagt cggccgcctc gcgggcctcg gccgccgcgc
     2941 gacggtcgac gaggtgcggg agttcttcgc cccgtacgcg ccgtacgagg ggctcgcggg
     3001 cgtgtacgcg ctttcgcggg cgaggggata ggcggtcagc gctcgatgtc tcgagagttt
     3061 cgctggctca gctgggcgtt gatggcgacg acgatcgccc tgctgtttcc tcggccaaga
     3121 caccgcggct ctcgttgttg ccacgttcgg cgtgggagtc gttctaaccg ggcgatatct
     3181 ctatcgacgg aggctctgag ggagtaaaga acggtggtga gaaaccttgg ggattcatgg
     3241 caggtggggt ggtcggcgca atagccgttt ggtaaacccg gaacctcgcg tttgcggtcg
     3301 cactcgtagt cgtattcgcg gtcgcgagcg tgattcggta tgtgctaatg cgcagagggc
     3361 tggctgagta cagccacggc atgttcggtc gctcaggcgc tgtcgacacg tgtggcggct
     3421 cggcccatac gtcgtcactt tcatcccata gtgggaagag atcgacgaca tccggcggct
     3481 tcgacccgaa cgtcgtcgtt cggggtgagt agttcaccct gagtggcgac gcggcaccct
     3541 cactgctaac cgacgaagcg cgcggcgcgg tccgaccgcc accctcagcc ctaacccgcc
     3601 gccgctggct ggttggccct catggcgtcg gccctcacct gcgcagcgcg gaccgctcga
     3661 gtgagggtgt cgacgtcata tgcgccctgg tgacgggtgc cgttgatgaa gaagctcggg
     3721 gtcccggcca cgccgctcgc gtcggcgctt gcgacgtcgt cggaaatccg ttcggcgtgc
     3781 ctgtggtggc ggatgtcgtc ccagaacttg tcgagatcga ggccgatctc ctccgcgtag
     3841 cgcccgagat ccttcggtcc gagctcgtcc tggtgggcga ggagcttgtc gttcatctcc
     3901 cagaacgcgc cctgcgcggc ggcggcctcg gatgcctcgg cggccatctg ggcattcgcg
     3961 tgcacgtcgt tgagagggag atggcgccac acgtagcgga gctcgtcccc gaacgagttg
     4021 agcagctctc gcaccacgac ctccgcctgc ccgcagtacg ggcactggta gtcaccgtac
     4081 tcgacgaggg tcaccggcgc gtccgggtcg ccgcggatgt gatcgcgctc gggatcgatg
     4141 tcctcggcca ggtccacgag ctcgtcgagg gtgccggcga tccgccgcgc acgcacctcc
     4201 gtgggcgtcc gcttgtacag gctgaccacg ccccagctga tcagggcagc gagcactgcc
     4261 gcagtgagaa cgccgatctt ggcctcctcg agtgtgtcgc cgtggaaggc gagggtggag
     4321 atcagcagcg agaccgtgaa cccgataccc gccacggccc cgagcgcggt gatcgccggc
     4381 cagctcaggc tgcggcgcag ccccagccgc gtcgcgatcc aagtcgcccc gagaatcccc
     4441 agtggcttgc cgagcacgta tccgaagatg atcccgagcg tgatggggga ggtgtaagcc
     4501 cgcccgagca ggtcgccatt gatgtggatt cccgcgttgg cgagcgcgaa cagcgggacg
     4561 atgacgaagc tggtccacgg atggagctcg tattgaaggc gctcgttcgc cgagatggcc
     4621 gaggccaccc cgcgctgcgc cgagcgcgcc agctcgggcg tcggttgctc gcggaacaag
     4681 cgcgtgagct cgaccacgcg ttcgaggcgg ccgcgttcgg gcgtgtaggc ggtggtcccg
     4741 agcccgatca gcagtcccga gatcaccgga tcgatccctg acttgtacag cgccacccac
     4801 agggccaccc cgagcaccgc cgcgatcggg ccgcggatgg cctgcggggc gtgccgcgac
     4861 gcgagcagca agccgaagat gacgacgccg gcgagcagcg acgtgacgtt gacgtgcttc
     4921 gtgtacacgg tggcgatcac gatcagggcc accaggtcgt cgaacactgc gaccgtgagc
     4981 agccgcacgc gcaggcgggt cccggccgga gcgagcaagg ccagcacgcc gagcgcgaaa
     5041 gcggtgtcgg tggacatcgc cgcgccccag cccttcgcgc cggcgccgcc ggcgttgaag
     5101 gcgaggtaga tcgcgatcgg cagcgccatg cccccgaggg ccgcgacgag cgggatcccg
     5161 acccgccggc gctcgcgcag ctgccctacg tcgagctcgc gcttggcctc gaggccgacg
     5221 acgaggaaga agagggtcat gaggccgttg ttgacccagt gccggaggtc ctgcgagatc
     5281 cccgagtcgc cgatccggat cgacaacgtg gtggtccaga cggactcgta ggagtggaac
     5341 cacggcgagt tcgcccacac gagcgccgcg accgcggcgg cgagcagcgc cagtgcgccg
     5401 ccggtctcgg tgttcaggta gtcgcgaagc ggcgccgtga ggttgcgcgc ccacgccgtc
     5461 ttttcgctaa agcgccgcgc cgctggaggc gtcgcctctg ctgtcgcgtc tacagccacg
     5521 tcactcacat agtgacgggc cgcgtcaagg cgctaaccgc ttttcagaag ccgccgtgcg
     5581 cgcgccatga atcccggtgg gcgcgcggtc gagagcgcgt accacgcctc gagcaggtcg
     5641 atgtcctcct ggcccgcgcg cttgaggaag cgctccgcgt gctcggactc gccgaacgcg
     5701 aagtgcgcga gcgagcggtg tcgctcgttc accaccgggc ggtcgtcgag atcggcgacc
     5761 gcgacggccg ccgcggagcg gagctcctcc acgagcacgc gctccaggtg ctccgccatc
     5821 gagccgatgt gggagtcgat cgcgtcgaaa agctgcccca gccagggatc ttctaccggc
     5881 gccgtctcat caggctggtt gcgccagacc ttgaaggctt cggtttgcac tccaacgggt
     5941 tcgttccccg tggctagatc cctgccccgg gcaggtactt gctcgtaaac gcgcccgccg
     6001 cgctgctcac ctgattgagg atgccgcttt cgctcatcca ctcggcgaac gagtcccact
     6061 ccttcccgtt catgtacccg aagggcttgt ccttgggcgg cagcatgaag ggaagcgtga
     6121 ccttcaccga tgcgcgctgc agcttggcgt cgaggtcggg gttcgccgcg agcagggcct
     6181 tgatcccctg ctcgggattc cggttcagct cgcgcgtgcc gcgcgcgagc gcgccgatga
     6241 agctgcggat cttcgcgccg ttctgctcga gcccgtccgc gttcgccacg agcacgagct
     6301 cgtcgtaggt cggcacgccg gccttctcga tccggatgat cttcgggtgc cgcttctgct
     6361 gagcgagctg tatgccctcg tagttccagt atccgccgag gatcgagtcg accttcttgc
     6421 tcacgagcgc gggcacgagg ttgaagccca cgttgcgctc cttcacccgc gacggcggca
     6481 cgttcgcgtt cttcaggatc gcctcgaggt acgcgtgctg gtagtcgatc cccgcggtgc
     6541 ccaccgtctt gcccgccagg tcggccggct tgtcgatcct cgccttcggc agcgagatga
     6601 tcgacgtgag cggcgtgcgc accagcgcgc cgaccgcgac gacgtgcagc ccctggtcgc
     6661 gtgctcgcaa cagctcgggc tcgtacgaga tggcgaggtc gacgcgcccc gccgccacct
     6721 gcttgagcgg cgcggccggg tcggacggcg tccggatctt cacgtcgagg ccgagcgact
     6781 tgaacaggcc ctgcgccttc gcctcgtaga tcccggcgtg gtccgcgttc gggaagaagt
     6841 cgagcatcag ctcgaacggc acagtcgcgc cgggcttgag cggttccttc ttctcgccgc
     6901 acccggcggc gccgacgcac agtgcgagcg ttgccagagc gaccgcgaac gcggacacac
     6961 gtctcctcat gcgggacttc ctcctcgttt ccaggggacg gcgacgcgct cgacgagcgt
     7021 cacgagcgcg aacagcgcca ccgccatcag ggtcaggatc acgacgccgg catacacgcg
     7081 cggcgtctgc agctggttgt tgccgagcag cacgagccgc cctaggccct tgtcggcgcc
     7141 cgcccactcg ccgaacacgg cgccgatgac ggagacggtc gccgccacgc gcaggccgct
     7201 gaagaagaaa ggcagcgacg acggcagctc cacgcgcact agcgtggcga cgcgtgaagc
     7261 gcgcaggctg cgcatgagct tgagcagctc ggggtcgacc gagcgcagcc catcgagcac
     7321 gttcaccgtg atcgggaaga agcagatgag cgcgacgatc gcgagcttcg ggccgatgcc
     7381 gtagtcgaag gcgagcacga acagcggcgc gagcacgacg atcgggatcg cctgcgaggc
     7441 gacgagcagc gggtaagccg cctcgcgcag cgggcggaag aggtgcatga ggatcgcgct
     7501 gccggcgccg gccagcaccg cgatcgcgag gccgagcagc acctcctcga gcgtcaccca
     7561 catgttgtcg accagcagcg cgtggtcgtg gcggatcgcg cgcagcgtct cggcgggcga
     7621 ggcgagcgtg aggttgtcga cgcccggcag gctggcgacc agctgccaga ggccgatgaa
     7681 cgctgcgaac agcagcaccg agagccacca gcgcctcatc cctcgagcgc ctccagcgcg
     7741 cggtgacgaa gctctgcgaa agccggggcc gcgatggcgt cgcgccgacg gccgcgcgga
     7801 cgggcgagcg gcaccgcgat ctcggccacc acccggccgg gacgtggcga gagcaccgct
     7861 acgcggtcgc cgaggtagat cgcctcctcc acgtcgtgcg tcacgagcac caccgtgcgc
     7921 ggctcgtgcg cgagcgcctc ggcgagccac tcctgcattg cgccgcgggt gagcgagtcg
     7981 agcgccgcga acggctcgtc gagcagcagc accgggcggc cggacaggag cgtgcgaagg
     8041 aaggcgacgc gctgccgcat gccgcccgag agctcggagg ggggcgactg ctcgaactcc
     8101 gccaggccga agcgctcgaa caagggggcc gcgcggcgcc gcgcctcctt gcgggagaca
     8161 ccctgaacct cgagcgcgag cgcggcgttg ccgaggccgt cgcgccacgg cagcagcagg
     8221 tcgcgctggg gcatgtaggc gcaggccgcg agccggtccg ccgcggcggc gtgcccgagt
     8281 gccgacaggg tgccggcgtc cggctcctgc aggccggcga tcagctcgag cagggtcgac
     8341 ttgccgcaac ccgatggccc cacgaccgcg agcacctccc cctcgcgcac gcgcaggtcc
     8401 accgcttcga gcgcgctcac ttcgccgcgg cgcgttctgt agcgccgtgc tagcccgctt
     8461 accgcgaccg cgtcttccga agaagagaga ggctccgtcg cgggagcctc gagatcagtc
     8521 tgcatcgctc cctccgcggg cattacccca caggttcgaa gggtcagcgc cgggcgatcc
     8581 tgtcgagggc gctatctcag cccgccttcc cgcgagcccc cctgactttt gcgcccttga
     8641 gtgtacgcct tggggctgta gcgttgtttc ataggagcag cgtttggccg gcgaactcgt
     8701 ctacttcgtg attccgactc gccaaggcga gcgcgccaag cgcttctacg gcgaactgtt
     8761 cggctggcgg ttcggcgagg gcaacgtgcc cggcggctcc cagatcgaga acacgaatcc
     8821 cgccggcggc atgttcgaga acccggaggg cactgtgccc caggtgtttt tccaggtgga
     8881 cgacatccac gcggccgtgg agcgggtgcg cgagctcggc ggcgagggag ccgagccgca
     8941 gagggtcgag tcgggctgga tggccgcctg ccgcgacgac cagggcgtcg agctgaatct
     9001 ctgggcgccg gcctcttgaa ccgcccgccc ggcgcgcggt acggtcggat cgcaagggta
     9061 agaggcgccg gtcttccgaa ggaggaccac gatgactgca agcactgtcg tccgcgagca
     9121 gggccagggg gaggcctact ggatgctcgg tgggctgtac gaggtgaagg tctccggggc
     9181 cgagtcagac ggtgagctca cgctcatgca gatgacgatc cccgccggca tggggccgcc
     9241 accgcacacg caccccggcg gcgagaccgt atgcgtgctc gacggtcgca tccgctacca
     9301 catcggcggc gagacccacg agggcgggcc ggggtcttgc ttccacgttc ccgccggcat
     9361 cgaggagaac ttcgagccgc tggaggagac gcgcctgctc gtggcgtaca cgcccggcgg
     9421 tgtcgaccgg ttcttcgccg aggccggtga gcccgccgcc cgtcgcgtgg tacctccgcc
     9481 gccggaggga ccgccggata tggcccgcct gatggagatc agcgagcggt acggggtcca
     9541 catacacgcg ccggtcgaga cctaaaagcg cttcacgtcg aagcagcgca tgtagacggc
     9601 gggcgcggac cgcccgccgt cggtgcgcac cgggccgtac gcgacgatca ggcccgttac
     9661 ccgagcccgc gacttcacgg gcgcgtccgg ctcgtcggct gtcgcacagt acgcgagcgc
     9721 gtcgctcgcc gggtccttca gctggagctc gcgcctcacg tccactcccg gagcctccgc
     9781 gcgctcgttg gcgaccacgg tccccgacac ggacacgtac ctgccatcga gcttctt
//
LOCUS       DASVXS010000174        15278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1390912, whole genome shotgun sequence.
ACCESSION   DASVXS010000174 DASVXS010000000
VERSION     DASVXS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390912"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VF752_09700"
     CDS             complement(<1..781)
                     /locus_tag="VF752_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8855864.1"
                     /translation="MQLHGGRLVAQRLAAHGVSHLFTLSGGHLFSIYDGCREEGIAIV
                     DTRHEQAAAFAAEGWAKATRAPGVCALTAGPGVTNGMSALASSLMNGSPVVALGGRAP
                     AMRWGQGSLQEIDHVPFVAPLVKSAQTAGATAEIPGLVDEALRLSLEPPTGPTFVDFP
                     LDVVFMEAPPSDAPAALPDPIAFPPADGDAIDRAAALLRDAERPVIMAGTGLYWAHGE
                     HALRALCEDLQVPVFLNGLARGCVPADHACFFSRARSQALKG"
     gene            866..1753
                     /locus_tag="VF752_09705"
     CDS             866..1753
                     /locus_tag="VF752_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8855865.1"
                     /translation="MSGGLGDDFGGTFPYEPRYLDADGTRLHYVDVGPRDGGAILMLH
                     GNPTWSYMYRTPIARLSERGHRCIAFDHMGFGRSDKPPEPKRYVLANHIANALEMIDG
                     LDLNDVTLVLHDWGGPIGLGAALERPDKVKAIVAMNTWAWELPSFLPPWLRQFRTEGL
                     GEILALANNVPVEAIPGGMYHRDTDGTMMEAYRAPFESYWTRVGTLGFIRDIPLTEHD
                     TSAPVIGAIHAELEAFDRPMHLIWGMRDPVFVPAFIEQWQELFPEAQATEIHTAAHYL
                     VEDEPEQVASAIAAFVETV"
     gene            complement(1750..3147)
                     /locus_tag="VF752_09710"
     CDS             complement(1750..3147)
                     /locus_tag="VF752_09710"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX8855866.1"
                     /translation="MAVVEIVTQDPWRRTIRDSLPPLEERLAFGRSLRNKIPRSELGK
                     WKLSPDRPDPVDLIEITNVGRVKELVPIRVGRMAASPLAFLRGAAAQMAADLASAPIS
                     GVVSWICGDAHLSNFGVYASPERSLVFDLNDFDEAVLGPREWDLKRLAASIVVAGRSR
                     GFSEDDSREAVLDAVRVYRLGMRELAGLPLVDTYRLRWKEDQTDVLGISKKSAKQGQK
                     LRQKAETRTNDQVLRKLTTTRKDGRWCFDEQPPLLTRLPEKDAERVASALERYTNTLP
                     PDRRDLVNAYAVQDAAFKVVGVGSVGTRAYVVALAGNGDRDALFLQVKEAPGSQLAPY
                     LPEHRVRHQGQRVVEAQLSLQALSDPFLGWTTIGRRHFYVRQLRDMKGSVDIETMRAR
                     GLIDYSRLLGGTLAMAHARCGDPAVIAGYLGKGDQVDRAIAEFAFAYADQTERDHDAV
                     VKAINAGRLPAEFGV"
     gene            complement(3195..3509)
                     /locus_tag="VF752_09715"
     CDS             complement(3195..3509)
                     /locus_tag="VF752_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855867.1"
                     /translation="MRLFRRRQPPVLYVHLANDGGIYVARGESGERFWTDGEGLRDEL
                     VRTQRRRGVLLYSRDDPGADPPPHVESMFESMLDFQLPLQLVEEPWPEALLPPEDREY
                     WP"
     gene            complement(3549..5129)
                     /locus_tag="VF752_09720"
     CDS             complement(3549..5129)
                     /locus_tag="VF752_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX8855868.1"
                     /translation="MKHDTHVAAIVIFCLLFGFVTVLGFVASRWRRARLDHINEWGLG
                     GRRFGNIVTWFLLGGDAYTAYTFIAVPALVFGAGALGFFALPYTIILYPIVFLIMPRL
                     WSVAHRHGYVTPADFVRGRYGSGTLASTVALTGLLATMPYIALQLVGIKVVLGAMGVQ
                     GDWPLIIAFAVLAAYTYNSGLRAPALIALVKDTLIYVTIIVAIVYITGKVGGFGHIFN
                     AAKEQLPNHTTATGKPGALIPGTHQAQTAFATLAFGSAMALFLYPHMVTGTLSAKSAD
                     VVRRNSIVLPAYTFLLGLIALLGYMAIAAGIKPSDPNFTVPDLFLKYFPPWFEGVAFA
                     AIAIGALVPAAIMSIAAANLFTRNIYKEYLKPDASDRTEANVAKIMSLFVKVGALLFV
                     ILLPTDFSINLQLLGGVWILQTLPAIVIGLYTRFFHKWALIAGWAAGMSLGTWMAVDL
                     EFKSSVHNVTIGGTTIAAYSGLWALALNLVVTAVLTVVFRVVGARDRGEDETRAEDYE
                     EVAAAAGPPEREPEAVPG"
     gene            complement(5126..5392)
                     /locus_tag="VF752_09725"
     CDS             complement(5126..5392)
                     /locus_tag="VF752_09725"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX8855869.1"
                     /translation="MNGNGRARRRWLYLYLLVPFVALLWVPFYAKEDPKLWGIPFFYW
                     YQFAWVIITVLLLAFVYVATRPPREYRAPSPEYRAPSRQGGEPL"
     gene            complement(5440..6648)
                     /locus_tag="VF752_09730"
     CDS             complement(5440..6648)
                     /locus_tag="VF752_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855870.1"
                     /translation="MREGVDRRAMAVLSTGHMMADTWQGAVPALLPFLIDERNYSYAA
                     VSGLVLAATVASSVIQPAFGHFSDRRSLAWLIPLGVLLGGVGIALVGVAESYPLTFLA
                     VVLSGIGVAAYHPEGSRFANYVSGEKRASGMSLFSLGGNIGFALGPVLVTPLAIAFGL
                     SGTLLLLVPTVLVTAWIVAELPRLVRFRPSEGPDAESPGSPDDDWPAFIKLGGVIAFR
                     TFAYFGLITFVPLYFIHELGTSKATGNLGLALMLIGGAVGTLIGGPLADRIGRRAVIV
                     ASMALSPPLIVLFQFLGEWPALVLAFVIGAVVISTFSVTVVMGQELLPGRIGVASGVT
                     LGLSVGLGGVSTPLLGVVADATSLSTVLWIVAALPLLGLAVALWLPSRPAARKRTRAR
                     SQRRVQQLQG"
     gene            complement(6651..7088)
                     /locus_tag="VF752_09735"
     CDS             complement(6651..7088)
                     /locus_tag="VF752_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8855871.1"
                     /translation="MTQTAPRTLRIGELAELTGTTPRTIRFYEEIGVLPAAEDREPGK
                     RRLYSEADVERLREVVRLRDLLGLSLEEVRELAEAESARAHIRREFQETDDPEERHRL
                     LEAALPYIERQLELVRGRIGELQKLEEELVARRKRVRTRLREQ"
     gene            complement(7085..8584)
                     /locus_tag="VF752_09740"
     CDS             complement(7085..8584)
                     /locus_tag="VF752_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8855872.1"
                     /translation="MEPATTTTARARARRPGVHAEHPNYKWWALSCTSLGMLMATINS
                     GTLIIALPDLERALGVSLLQLVWVILVYMIASTVLVLTAGRLSDLFGRKKAYVLGFLV
                     FSLASLGAGFSTGATELLIWRVLQGIGGAFLFANSSAIVTDAFPREQLGVAMGTNTMV
                     AAVGLVIGPVLGGALVAISWHWVFWFNVPFGLIGSLWAGLVLHELAGRDENRGLDLGG
                     TAVYVVGLTGLVYALSRAGLSGWNDPLVIAGLVAAAILLPLFVVIENHQRAPMLDLKL
                     FKDKMFAAASGAAFINGLSRFALLFIFVFYFQGPQGDDPITAGIKLAPMAIGMLISSP
                     IAGLVADRCGSRLLAAGGMLVTALGLALMTTLQVHTAYWESGLYLFIVGIGSGMFNSP
                     NTAAMMGGVPGKRRGVAAGARVMLQNTGAVISIAFVIAVVTNAVPKDVLFKIFSGLTK
                     GLSATQLDPFISHMHLALWCLAGVSLLGAFVSLLRPSHSAAAELEPARA"
     gene            complement(8646..9212)
                     /locus_tag="VF752_09745"
     CDS             complement(8646..9212)
                     /locus_tag="VF752_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855873.1"
                     /translation="MARHDVEPVRQRVWVRERSSRTLDQRLNLRFTGIARISARLVAK
                     LSPRSRIRQAALLRAVRLSFEAYNRRDLDAAVAGWDPDFEYFPDRDWVRAGLVEPSYR
                     GVEGYRTYIATADEVWGGQIEPLEVIDTGEQFVVLGEIAGTMRGQARGVPLTQQYVGV
                     VSLSDGIPVRCDEYFDRAKGLAAVGLDD"
     gene            complement(9265..9831)
                     /locus_tag="VF752_09750"
     CDS             complement(9265..9831)
                     /locus_tag="VF752_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855874.1"
                     /translation="MARQNVEPVRQRLWAHERSSRTLDQRLYLRFPRLGALGARLFAQ
                     LPPRSRLRQAALLRATRLSLEAYNRRDLKAVVVGWDPEFEYIPDRQWVDAGLVEPSYH
                     GLAGYRRFIATADEVWGEENELHPIEVIDFGDRLLVRADVTMRAQVSGVPLTQPWGAL
                     CTLRNGMVIRLEEHFDHARALAAVGLSG"
     gene            complement(10043..10456)
                     /locus_tag="VF752_09755"
     CDS             complement(10043..10456)
                     /locus_tag="VF752_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855875.1"
                     /translation="MEEGPSASVVRGSWDAFQTGGIDAMAEFWHPDIEWRAMEGAADD
                     AGVMRGRARLRRYYQDWLDTIDYELVELDEVALEEGERVVALVHTVGRGRLSGAPLEG
                     RYAIAYTLRDGRIVSGREYGNAEDAIAASDLAKGQ"
     gene            10558..11241
                     /locus_tag="VF752_09760"
     CDS             10558..11241
                     /locus_tag="VF752_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855876.1"
                     /translation="MGRFGALLAGAMAICALALPATAGAQAAGDIKVRLGAHHYPRPW
                     GLTYVYGYIRVAKGADETIRRGKQVTLEQSVWPFTSWQPIGSTTTEFNGYFSFRRWNI
                     INNQKFRATAQLDSGAYVSQVLVLHVPFRARMTVSVRKAKKAALVTVSGKVRPARPGK
                     TVAIQRRSPTSGWRTVTRTKLRGATAGSSSFKSRPMRIRHDSIFRVRVRSDALNSVGM
                     SGGRLVHVR"
     gene            complement(11256..13496)
                     /gene="hypF"
                     /locus_tag="VF752_09765"
     CDS             complement(11256..13496)
                     /gene="hypF"
                     /locus_tag="VF752_09765"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX8855877.1"
                     /translation="MSAVAHAVTRLRARVEGTVQGVGFRPHVYRLATELGLGGWVLND
                     ERGVLLEVEGAPERVEAFILRLTAEAPPLASVQRVLRESVPPEGSRGFEIHESERSGE
                     PAALVTPDSATCDDCLGELFDPADRRFRYPFINCTNCGPRFTIVRGVPYDRPLTTMAS
                     FPMCERCRREYEDPADRRFHAQPNACPECGPWLRLVDRTGAAVTSSDPIRTVAATLSA
                     GLVVAVKGLGGFHLACVASDEPVVASLRARKQRESKPFALMVPSVGAARELVELSEEE
                     EALLLGRERPIVLARRWDGAPVANAVAPGLPELGLMLPYSPLHHRLMSECGVPLVMTS
                     GNVSDEPIAYRDHSALARLRGIADLFLLHNRPIHMRTDDSVVRVVHSRPLMLRRSRGY
                     VPASIDLPLETPPLLACGAELKSTFCVAKGTRAWVGHHIGDLKNWETLRSFREGVEHF
                     ERLFAVAPERVVHDLHPDYLSTRYASEREGVMLVGVQHHHAHLAACLAEHGETGRAVG
                     AIFDGSGYGTDGTVWGGEILVGDLTGFERVGHLWPARLPGGDRAAEQPWRMACAWLVE
                     AFGDEGAALPGVDPAQWAAIGHMARSGFSSPLTTSVGRLFDAVAALSGVRTESDYEGH
                     AAIELEAAAARAERGAYPFPGLDAREAIRAVCADLAGRTDPGVVSARFHNGLAVATAH
                     ACAEAAEGAGVDLVVLSGGVFQNRRLLEATAELLRAMRLRVLVPERLPPNDGGISYGQ
                     AAVAAA"
     gene            complement(13493..14626)
                     /locus_tag="VF752_09770"
     CDS             complement(13493..14626)
                     /locus_tag="VF752_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX8855878.1"
                     /translation="MSDAVAAPSERTEANARFFEAEAPRIAELCRQMAERFARGGRLI
                     AIGATPAARSDVRHIAVEFVHPVIVGKRALPAIGLSLEGGPLAPQVELLAEPDDIVVG
                     FGEDVAALAPAAERGCLTVAFEHEFPPPTQDPFIRQELIETLYHVLWELVHVFFDHLD
                     RGMEGTGASSFLYPFLGEQEHDLDAVVADVARSVRMKAEEVGALRVQTLTEGASALAA
                     AAAVLRRSFDASGKLLALGNGGSATDAMDAVADFRSAPFHWPARPAIDLTEDAAILTA
                     LANDIGTDAIFARQVIAYGREGDVLLALSTSGNSSNVIEALNEARRRSLHTVAMVGYD
                     GGRVAAERLADHVVITRSQHIPRIQEAQASAYHELRRLVEAAG"
     gene            complement(14623..>15278)
                     /gene="hypB"
                     /locus_tag="VF752_09775"
     CDS             complement(14623..>15278)
                     /gene="hypB"
                     /locus_tag="VF752_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX8855879.1"
                     /translation="EDALDANNTIARANRSDLDRASVKVVNLMSAPGAGKTSLLERVL
                     GDVGDVRAGVLEGDVQGSLDADRLAWLHVPVTQLNTDPGFGGECHLDANMVRSALPEI
                     PLPELDLLVIENVGNLVCPAEFRVGEDARVMVSSITEGEDKPLKYPLMFRTCELVLIN
                     KIDLLPHLDFDVERFLCNLDKVNPDSRRILTSARTGEGVEQFRDWLVQLAAREEARV"
BASE COUNT         2517 a         5392 c         5228 g         2141 t
ORIGIN      
        1 cgcccttcag cgcctgcgag cgagcgcgtg agaagaagca cgcgtggtcc gccggtacgc
       61 agccacgcgc gagcccgttc aagaagacgg gcacctgcaa gtcctcgcac agcgcgcgca
      121 gcgcgtgctc gccgtgcgcc cagtagagcc cggtgccagc catgatcacc ggccgctcgg
      181 cgtcgcgcag cagcgcggcg gcgcggtcga tcgcgtcgcc gtcggcgggc ggaaacgcaa
      241 tcggatccgg cagagcagca ggcgcgtcgg atggcggcgc ctccatgaac acgacgtcga
      301 gcggaaagtc gacgaaggtc ggcccggtcg gcggctccag agagagccgc agcgcctcgt
      361 ccacgagccc ggggatctcg gcggttgcgc ccgctgtctg cgcactcttg accaggggcg
      421 ccacgaacgg aacgtggtcg atctcctgca gcgatccctg cccccagcgc atcgccggcg
      481 cgcggccgcc cagggcgacg acaggcgagc cgttcatcag cgaggaggcg agcgcgctca
      541 tgccgttcgt caccccaggg ccggccgtca gggcgcagac gccgggcgcg cgcgtagcct
      601 tcgcccagcc ctcagcggcg aacgccgccg cctgctcgtg ccgcgtgtcg acgatcgcga
      661 tgccctcttc gcggcagccg tcgtagatcg agaagagatg gccgcccgag agggtgaaga
      721 ggtgcgacac gccgtgcgcc gcgagccgct gcgccacgag ccgcccgccg tgcagctgca
      781 cctgctcaga ttccgaagcg gcgacctgtt cccgggctga cacgagcggc tcgggagact
      841 acccgagcgc gctataaccg tggccgtgag cggcggtctc ggagacgact tcggcggcac
      901 cttcccgtac gagccgcgct atctcgacgc cgacggcacg cgcctccact acgtggacgt
      961 tggcccgcgc gacggcggcg cgatcctcat gctccacggc aatccgacct ggagctacat
     1021 gtaccgcacg ccgatcgcgc gcctgtcaga gcgcgggcac cgctgcatcg cgttcgacca
     1081 catgggcttc ggccgctccg acaagccgcc cgagccgaag cgctatgtgc tcgcgaacca
     1141 catcgccaac gcgctcgaga tgatcgacgg cctcgacctg aacgacgtga cgctcgtgct
     1201 ccacgactgg ggcgggccga tcggactggg cgcggcgctc gagcgtccgg acaaggtcaa
     1261 ggcgatcgtc gccatgaaca cctgggcctg ggagctgccg agcttcctgc cgccgtggct
     1321 gcgccagttt cgcaccgagg gcctcggcga gatcctcgcg ctcgcgaaca acgtgccggt
     1381 cgaggcgatc cccggcggga tgtaccaccg cgacaccgac ggcacgatga tggaggccta
     1441 ccgcgcgccg ttcgagagct actggacgcg cgtcggcacg ctcggcttca tccgcgacat
     1501 cccgctcacc gagcacgaca cgagcgcgcc cgtgatcggc gcgatccacg ccgagctcga
     1561 ggccttcgac cgccccatgc acctgatctg gggcatgcgc gaccccgtgt ttgtgcccgc
     1621 cttcatcgag caatggcagg agctcttccc ggaggcgcag gcgaccgaga tccacaccgc
     1681 cgcgcactat ctggtcgagg acgagcccga gcaggtcgcg agcgcaatcg ccgccttcgt
     1741 cgagacggtc tagacgccga actcggccgg cagccgccca gcgttgatcg ccttgacgac
     1801 cgcgtcgtgg tcgcgctcgg tctggtcggc ataggcgaag gcgaactcgg cgatcgcgcg
     1861 atccacctgg tcgcccttgc cgagatagcc ggcgatcacc gccgggtcgc cgcagcgcgc
     1921 gtgcgccatc gcgagcgtgc cgccgagcaa cctcgagtag tcgatcagcc cgcgggcgcg
     1981 catcgtctcg atgtcgaccg agcccttcat gtcgcgcagc tggcgaacgt agaagtgccg
     2041 ccggccgatc gtggtccagc cgaggaatgg atccgacagc gcctgcagcg acagctgcgc
     2101 ctccaccacg cgctggccct ggtggcgcac gcggtgttcg ggtagatacg gcgcgagctg
     2161 cgagccgggc gcctccttca cctgcaggaa cagcgcgtcc cgatcgccgt tccccgccag
     2221 cgccaccacg taggcgcgcg tgccgacgct cccgacgccg accaccttga acgccgcgtc
     2281 ctgcaccgcg taggcattca cgaggtcgcg gcggtcaggc ggcagcgtgt tcgtgtagcg
     2341 ctcgagcgcc gacgcgaccc gctcggcgtc cttctccggc aggcgcgtga gcaggggcgg
     2401 ctgctcgtcg aagcaccagc gcccgtcctt ccgcgtggtc gtgagcttgc gcagcacctg
     2461 gtcgttcgtg cgcgtctccg ccttctgcct cagcttctgc ccctgcttcg cggacttctt
     2521 cgagatcccg aggacgtccg tctggtcctc cttccagcgg agccggtagg tgtcgaccag
     2581 cggcagcccg gccagctcgc gcatgccgag ccggtagacc ctcacagcgt cgagcaccgc
     2641 ctcacgcgag tcgtcttcgg agaagccgcg gctccggccg gccaccacga tgctcgccgc
     2701 gagccgcttc aggtcccact cccgcggccc gagcacggcc tcgtcgaagt cgttcaggtc
     2761 gaacacgagc gagcgctcgg gcgacgcgta cacgccgaag ttcgagaggt gagcgtcgcc
     2821 gcagatccag gaaacgacac cggagatcgg cgcgctcgcg aggtcggccg ccatctgggc
     2881 tgccgccccg cgcaggaacg cgagcggcga cgccgccatg cgacccacgc ggatcggcac
     2941 gagctccttc acgcggccga cgttggtgat ctcgatcagg tcgaccggat cgggccggtc
     3001 aggcgagagc ttccacttgc cgagctcgct tcgcgggatc ttgttgcgca gcgagcgacc
     3061 gaacgcgagg cgctcctcga gcgggggcag tgaatcccgg atcgtccgtc tccagggatc
     3121 ctgcgtgact atctcgacga ccgccatgcg cgctgctggg tatacctggg atcacgcgcc
     3181 gaatccagtc ctctttacgg ccagtactcg cggtcttcgg gcggcaagag cgcctccggc
     3241 cacggctcct ccacgagctg cagcggcagc tggaagtcga gcatcgattc gaacatcgac
     3301 tcgacgtgcg gcggcgggtc ggcgccggga tcgtcgcgag agtagagcag cacgccgcgc
     3361 cggcgctgcg tgcgcacgag ctcgtcgcgc agcccttcgc cgtcggtcca gaagcgctcg
     3421 ccgctctcgc cgcgcgcgac gtagatgccc ccgtcgttcg cgaggtggac gtagaggacg
     3481 ggcggctggc gacggcggaa cagcctcacc gcagtgaggc tatcccgcca taccagtgct
     3541 gagtaaggct aacccggaac ggcttccggc tcgcgctcgg gcgggcccgc cgcggcggcg
     3601 acttcctcgt agtcctccgc gcgcgtctca tcttcgccgc gatcgcgggc tccaacgacc
     3661 cggaacacga ccgtgagcac agccgtcacg acgaggttca gcgccagggc ccacaggccc
     3721 gagtaggccg cgatagtggt gccgccgatc gtgacgttgt ggaccgagga cttgaactcg
     3781 aggtcgaccg ccatccacgt gcccagcgac atgcccgccg cccagccggc gatcagcgcc
     3841 cacttgtgga agaagcgcgt gtacaggccg atgacgatcg cgggcagggt ctgcaagatc
     3901 cagacaccgc cgagcaactg caggttgatc gagaagtccg tgggcagcag gatcacgaac
     3961 agcagcgcac ccaccttcac gaacaacgac atgatcttcg cgacgttcgc ctccgtgcgg
     4021 tcggacgcgt cgggcttcaa atactccttg tagatgttgc gcgtgaacag gttggccgcg
     4081 gcgatcgaca tgatcgcggc aggcacgagc gcgccgatcg cgatcgcggc gaacgcgacg
     4141 ccctcgaacc acggcgggaa gtacttcagg aacaggtccg gcaccgtgaa gttcggatcg
     4201 ctcggcttga ttccggcggc gatcgccatg tacccgagca gcgcgatcag gccaagcagg
     4261 aacgtgtacg cgggcagcac gatcgagtta cggcgcacca cgtccgcgct cttcgcactg
     4321 agcgtgcccg taaccatgtg cggatacagg aacagcgcca tcgccgagcc gaacgccagc
     4381 gtggcgaagg cggtctgcgc ctggtgggtg cccgggatca acgcacccgg cttgcccgtc
     4441 gcggtcgtgt ggttcggcag ctgctccttg gcggcattga agatatggcc gaagccgccg
     4501 accttgccgg tgatgtatac gatcgcaacg atgatcgtca cgtaaatcag cgtgtccttc
     4561 accagggcga tcagcgctgg ggcgcgcagg cccgagttgt acgtgtacgc ggcgagcacg
     4621 gcgaacgcga tgatcagcgg ccagtcgccc tggacgccca tcgccccgag caccaccttg
     4681 atgcccacga gctggagcgc gatgtacggc atcgtggcga gcagccccgt cagggccacg
     4741 gtgctcgcaa gcgtgccgct gccatagcgg ccgcgcacga agtcggccgg tgtgacgtag
     4801 ccgtggcggt gcgccaccga ccacagccgc ggcatgatca ggaacacgat cggatagagg
     4861 atgatcgtgt acggcaaggc gaagaagcca agcgcgccgg caccgaacac gagcgccgga
     4921 acggcgataa atgtgtacgc cgtgtaggcg tccccgccga gcaggaacca cgtcacgatg
     4981 ttgccgaagc gcctgccgcc aaggccccat tcgttgatgt ggtcgagccg cgcgcgccgc
     5041 cagcgcgacg ccacgaaccc gaggacggtc acgaatccga agagcaggca gaagatgacg
     5101 atcgcagcga catgggtgtc gtgtttcaca gcggctcacc accctggcgc gacggtgcgc
     5161 ggtactcagg gctgggtgcg cggtactcgc gcggcgggcg tgttgccacg tacacgaatg
     5221 ccagcagcag caccgtgatg atcacccagg cgaactggta ccagtaaaag aacggaatgc
     5281 cccacagctt cgggtcctct ttcgcataga acggcaccca caggagtgca acgaaaggaa
     5341 caagcaggta gaggtacagc cagcgtctgc gagcccggcc attgccattc atcagcgacc
     5401 tccctcgctg aattggggcc ccggctcccg ctggggcccc tatccttgga gttgctgtac
     5461 ccgacgctgt gagcgtgcac gcgtccgttt gcgggcggcg ggtctgctcg gtaaccacag
     5521 cgccacggcg agccccagca gcggcaacgc cgcaacgatc cacaacacgg tgctgagcga
     5581 agtggcgtcg gcgacgacgc cgagcagcgg cgtgctgacg ccgccgagcc cgaccgagag
     5641 cccgagcgtg accccgcttg caacaccgat gcggcccggc aggagctctt gccccatgac
     5701 gacggtgacg ctgaaggtgg agatcacgac ggcgccgatc acgaacgcga gcacgagcgc
     5761 cggccactcg cccaagaact ggaagagcac gatcagcggc gggctgagcg ccattgaggc
     5821 gacgatcacg gcgcgacggc cgatgcgatc tgcgagcggg ccgccgatca gcgtgccgac
     5881 cgcgccgccg atcagcatca gcgcgagccc gaggttgccg gtggccttcg acgtgccgag
     5941 ctcgtggatg aagtacagcg gcacgaacgt gatcagcccg aagtacgcga acgtgcggaa
     6001 cgcgatcacg ccgccgagct tgatgaacgc gggccaatcg tcgtccggcg agccggggct
     6061 ctcggcatcc ggcccttcgc ttggccggaa gcgaacgagc cgtggcagct cggccacgat
     6121 ccaggcggtg acgagcacgg tcgggacgag cagcaggagc gtcccgctga gcccgaacgc
     6181 gatcgcgagc ggggtcacca gcaccggccc gagcgcgaag ccgatgttcc cgccgagcga
     6241 gaacaggctc atgcccgagg cgcgcttctc gcccgaaacg tagttggcga accgcgagcc
     6301 ctccgggtgg taggccgcca caccgatgcc gctcagaacc accgcgagga acgtgagcgg
     6361 gtacgactcg gccacaccca cgagagcgat gccaacgccg ccgagcagga caccgagtgg
     6421 gataagccac gcgagcgagc ggcggtcgga gaagtggccg aaggcgggct ggatcaccga
     6481 tgacgccacc gtggccgcca gcaccaggcc ggacaccgcg gcgtacgagt agttgcgctc
     6541 gtcgatcagg aagggcagga gcgccgggac ggcgccctgc caggtgtcgg ccatcatgtg
     6601 gcccgtcgag aggaccgcca tcgcccggcg gtccacaccc tccctcacga ttactgctcc
     6661 cgcagccggg tcctcacgcg cttgcggcgc gcgacgagct cttcctccag cttctggagt
     6721 tccccgatcc gcccgcgcac aagctcgagc tggcgctcga tgtacgggag cgcggcctcg
     6781 agcaggcggt gccgctcctc tgggtcgtcg gtctcctgga actcacggcg gatgtgggcg
     6841 cgggcgctct cggcctccgc gagctcgcgt acctcctcga gcgagagccc gagcaggtcg
     6901 cgcagccgaa cgacctcgcg gaggcgctcg acgtcggcct cggagtagag ccggcgcttg
     6961 cccggctcgc gatcctccgc cgctggcagc acgccgatct cctcgtagaa gcggatcgtg
     7021 cgcggcgtgg tgccggtcag ttcggccagc tcgccgatgc gcagcgtgcg gggcgcggtc
     7081 tgggtcatgc gcgcgcaggc tccagctcgg cggccgcgct gtgcgacggc cgcagcagtg
     7141 agacgaaggc gccgagtagc gacacgcccg ccaggcacca cagcgccagg tgcatgtgcg
     7201 agatgaacgg gtcgagttga gttgcgctca gacccttggt gaggccggag aagatcttga
     7261 acagcacgtc cttcggcacc gcgttggtga cgacagcgat cacgaacgcg atcgagatga
     7321 ccgcgccggt gttctggagc atcacacgcg caccggcggc gacgccgcgg cgcttgccag
     7381 gtacgccgcc catcatggcg gcggtgttcg ggctgttgaa catgcccgag ccgatcccga
     7441 cgatgaacag gtagagcccg ctctcccagt aggcggtgtg cacctggagc gtggtcatga
     7501 gggccagccc gagagcggtc acgagcatgc caccggccgc gagcagccgc gacccgcagc
     7561 ggtcggcgac gaggcccgca atcgggctcg agatcagcat gccgatcgcc atcggcgcca
     7621 gctttatacc ggcggtgatc ggatcgtcgc cctgcggacc ctggaagtag aagacgaaga
     7681 tgaacagcag cgcgaagcgg ctgagcccgt tgatgaacgc ggcgccggaa gcggccgcga
     7741 acatcttgtc cttgaagagc ttgaggtcga gcatcggcgc gcgctggtgg ttctcgatca
     7801 ccacgaagag cggcaagagg attgcggcag cgacgagccc ggcgatcacg agtgggtcgt
     7861 tccagccgga gaggcccgcc ctcgacagcg cgtagaccag gccggtgagg cccacgacgt
     7921 agacggctgt cccgcccagg tcgaggcccc ggttctcgtc gcggcccgcg agctcgtgga
     7981 ggaccagccc ggcccagagc gagccgatca gcccgaacgg cacgttgaac cagaacaccc
     8041 agtgccagct gatcgcgacg agcgcgccgc cgagcaccgg cccgatcacg agaccgaccg
     8101 cggcgaccat cgtgttcgtg cccatggcca cgccgagctg ctcgcgcggg aaggcgtcgg
     8161 tgacgatcgc cgagctgttc gcgaacagga aggcgccgcc gatgccctgc agcacgcgcc
     8221 agatcaacag ctcggtggcg cccgtcgaga agccggcgcc gagtgacgcc aggctgaaga
     8281 cgaggaagcc cagcacgtag gccttcttgc ggccgaagag gtcgctcagg cggcccgcgg
     8341 tgagtaccaa caccgtgctg gcgatcatgt agacgaggat cacccacacg agctgcagca
     8401 gcgagacgcc gagcgcgcgc tcgagatcgg gcagggcgat gatcagggtg cccgagttga
     8461 tcgtggccat gagcatgccc aggctcgtgc agctgagcgc ccaccacttg tagttcgggt
     8521 gctcggcgtg cacgccgggc cggcgcgcgc gtgctcgagc ggtggtggtc gtggcgggct
     8581 ccatgctctt tcttacgtaa acgtaaggtt agcaaggccg gggcccgggc cgagggccgg
     8641 cgcggctaat cgtcgaggcc gaccgcggcg aggcccttgg cgcggtcgaa gtactcgtcg
     8701 cacctgacgg gtatgccgtc cgacagggag accacgccta cgtactgctg tgtgagcggc
     8761 acgccacgcg cctggccgcg catggttccc gcgatctcgc cgagcaccac gaactgctca
     8821 ccggtatcga tcacctcgag cggctcgatc tgcccgcccc acacctcgtc ggcggtcgcg
     8881 atgtatgtgc ggtacccctc gacgccgcgg tagcttggct cgacgaggcc ggcccgaacc
     8941 cagtcgcggt cggggaaata ctcgaagtcc gggtcccatc ccgcgaccgc cgcgtcgaga
     9001 tcgcggcggt tgtaggcctc gaatgagagg cgcacggcgc gcaacagcgc ggcctggcgg
     9061 atccgtgagc gaggcgacag tttcgcgacc agccgagcgg aaatcctcgc gatgcccgtg
     9121 aagcgcaggt tcaagcgctg gtcgagggtg cggctcgatc gctcgcgcac ccagacacgc
     9181 tggcgcacgg gctcgacgtc gtggcgggcc atcggaagcg cagtatctcg cttgcgcagc
     9241 gaggcgccag gcgtctttcg atcgctagcc cgataggccc acggccgcga gcgcccgtgc
     9301 gtggtcgaag tgctcctcga gcctgatgac catcccgttc ctgagcgtgc agagcgctcc
     9361 ccacggctgc gtgagcggga cgccactgac ctgggcgcgc atcgtcacgt ctgcgcgcac
     9421 tagcaaacgg tcaccgaagt cgatcacttc tatcgggtgc aactcgttct cctcgcccca
     9481 cacctcgtcc gcagtcgcga tgaacctgcg gtagcccgcc aggccgtgat agctcggttc
     9541 caccaggccc gcgtccaccc attggcggtc ggggatgtac tcgaactccg gatcccaccc
     9601 gaccaccacg gctttcaggt cgcgccggtt gtacgcctcg agggagaggc gcgtggctcg
     9661 cagcagcgcg gcttggcgga gacgcgagcg cggcggcagc tgagcgaaca ggcgagcacc
     9721 cagcgctccg agtcgtggga aacggagata gaggcgttga tcgagcgtcc ggctcgaccg
     9781 ttcgtgcgcc cagagccgct gacgcaccgg ctcgacgttc tggcgggcca tctggtgtgc
     9841 agtatctacc gcgcccggag ccggggttcg aaagttccat atcgaccccg gcacagcgac
     9901 gacatggaag ttatgcaccg tatgggtgca tttctcccat ctcgtttcgg cgaatcgccg
     9961 gagatggacc ttatgcaccc cgctcgcgct cccctaagtg ctaaaccgcg ccgccacagc
    10021 acttgagggc ggcggccgcg cctcactggc ccttcgccaa atcgctcgcg gcaatcgcgt
    10081 cttcggcgtt gccgtactcg cggccgctca cgatgcggcc gtcgcgcagc gtgtaggcga
    10141 tcgcgtagcg accttcgagc ggcgcgccgc tcaggcgtcc ccgcccgacc gtgtgcacca
    10201 gcgctactac gcgctcaccc tcctcgagcg caacttcgtc gagctcgacc agctcgtaat
    10261 cgatcgtgtc gagccagtcc tggtagtagc gccgtagcct cgcgcggccc cgcatcacgc
    10321 cggcatcgtc ggcggcaccc tccatcgcac gccactcgat gtcgggatgc cagaactcgg
    10381 ccatcgcatc gattccgccg gtctggaacg cgtcccacga cccgcgtacc accgaggcgc
    10441 tcgggccctc ctccatggtg cgcagtatcc cgcgccacag ccacgacgag gccgacaccg
    10501 caacctccgg cgctccggat cgtttgagtg gcaatggcag ggggcagaac gcattccgtg
    10561 ggacgcttcg gcgcgctcct ggcaggggca atggcgatat gcgcgctcgc gctgccggcg
    10621 acggccgggg cgcaggcggc cggcgacatc aaggtgcgcc tcggcgcgca tcactacccg
    10681 cgcccatggg gcctgaccta cgtctacggc tacatccggg tggccaaagg cgccgatgaa
    10741 acgatccgcc ggggcaagca ggtcacgctc gagcagtcgg tgtggccatt cacctcgtgg
    10801 cagccgatcg gctcgaccac gaccgagttc aacggctact tcagcttccg gcgctggaac
    10861 atcatcaaca accagaagtt ccgcgccacg gctcagctcg actcgggcgc atacgtgagc
    10921 caggtgctcg tgctgcacgt gccgtttcgc gcgcggatga ccgtgtcggt gcgcaaggcg
    10981 aagaaagccg ccctggtgac ggtcagcggc aaggtgcgcc ccgcgcggcc cggaaagacc
    11041 gtcgcgatcc agcgacgctc gcccacgagc ggctggcgca ccgtgacgcg cacgaagctg
    11101 cgcggcgcga cggccggctc ctcgagcttc aagagccggc cgatgaggat ccgccacgac
    11161 tcgatcttcc gcgttcgtgt gcggagcgac gcgctcaact cagtcgggat gagcggcggt
    11221 cggctcgtgc acgttcggta acaccaggca gctcctcagg ccgccgccac cgctgcctgg
    11281 ccgtaggaga tgccgccgtc gttcggcggc aggcgctcag gcacgagcac gcgcaaccgc
    11341 atggcgcgca gcagctcggc cgtcgcttcg agcagacgcc ggttctggaa cacgccgccc
    11401 gatagaacga ccaggtcgac gcccgcgccc tccgccgcct cggcacacgc gtgcgcggtc
    11461 gcgaccgcga ggccgttgtg gaagcgcgcc gagaccactc cgggatctgt gcgaccggcg
    11521 aggtcggcgc aaacggcgcg gatcgcctct cgggcgtcga ggccggggaa cggataggcg
    11581 ccgcgctcag cgcgcgccgc cgccgcctcg agctcgatgg cggcgtgccc ctcgtagtcc
    11641 gactccgtgc gaacgccgga gagtgccgcc accgcgtcga acaggcgccc gacactggtc
    11701 gtgagcgggg acgagaagcc cgagcgggcc atgtgcccaa tagccgccca ctgagccgga
    11761 tcgaccccag gcagcgccgc gccctcgtcg ccgaacgcct caaccagcca ggcgcacgcc
    11821 atgcgccagg gctgctcggc ggcacggtcg ccgcccggca gtcgcgccgg ccacaggtgc
    11881 ccgacgcgct cgaagccggt gaggtcgccg accaggatct cgccgcccca caccgtgccg
    11941 tccgtgccgt agcccgagcc gtcgaagatc gcccccaccg ctcgtcccgt ctcgccgtgc
    12001 tcggcgaggc aggccgccag gtgagcgtga tgatgctgca cgcccaccag catcacgccc
    12061 tcgcgctcgg atgcgtagcg ggtagagagg tagtcgggat gcaggtcgtg cacgacgcgc
    12121 tccggggcga ccgcgaacag gcgctcgaag tgctcgacgc cctcgcggaa cgagcggagc
    12181 gtctcccagt tcttcaggtc gccgatgtgg tggcccaccc acgcgcgcgt ccccttcgcg
    12241 acgcagaacg tgctcttcaa ctcggcgccg caggcgagca gcggcggcgt ctcgagcggc
    12301 aggtcgatgc tcgccggcac gtagccgcgc gagcgccgca gcatcagcgg gcggctgtga
    12361 accacacgca ccactgagtc gtcggtgcgc atgtgaatcg gccggttgtg gagcaggaag
    12421 aggtcggcga tgccgcgcag ccgcgcgagc gcgctgtggt cgcggtaggc gatcggttcg
    12481 tcggacacgt tgccgctcgt catgacgagc ggaacgccgc actcggacat gagccgatgg
    12541 tggagcggcg agtacggcag catcaggccg agctccggca accccggcgc caccgcgttc
    12601 gcgaccggtg cgccgtccca ccggcgcgcc agcacgatcg gccgctcgcg tccgagcagc
    12661 agcgcctcct cctcttccga aagctccacc agctcacgcg cggcacccac gctcggcacc
    12721 atcagcgcga acggcttgct ctcccgttgc ttgcgtgccc gcagcgaagc gaccaccggc
    12781 tcgtcggacg ccacgcacgc gaggtggaat ccgcccagcc ccttcaccgc caccacgagg
    12841 cccgcgctaa gcgtggccgc gacggttcgg atcgggtccg agctcgtgac cgcggcgccg
    12901 gtccggtcca cgagccgcag ccacggcccg cactccgggc aggcgttcgg ctgggcgtgg
    12961 aagcgccggt cagccgggtc ctcgtactcg cgccggcagc gctcgcacat cggaaacgac
    13021 gccatcgtcg tgagcggccg gtcgtaggga acgccgcgca cgatcgtgaa gcgcggcccg
    13081 cagttggtgc agttgatgaa cgggtagcga aagcgacggt cggccgggtc gaagagctcg
    13141 ccgagacagt cgtcacatgt ggcgctgtcc ggcgtgacga gcgcggcggg ctcgccggag
    13201 cgctcgcttt cgtggatctc gaagccgcgc gagccctcgg gtggcacgga ctctcgtagc
    13261 acccgctgca ccgacgcgag cggcggcgct tcggccgtca gccgcaagat gaacgcctcc
    13321 acgcgctcgg gcgccccctc cacctcgagc aatacgccgc gctcgtcgtt cagcacccat
    13381 ccgccgagcc cgagttcggt ggcgagccgg tacacgtgcg gcctaaagcc gacaccctgg
    13441 acggtgccct ccacgcgcgc gcgaaggcgc gtgacagcgt gggcgaccgc gctcaccccg
    13501 cggcctccac gagccggcgc agctcgtggt acgcgctcgc ctgcgcctcc tggatgcgcg
    13561 ggatgtgctg cgagcgcgtg atcacgacgt ggtcggcgag ccgctcggct gccacgcgcc
    13621 cgccgtcgta gcccaccatc gcaaccgtat gaaggctgcg gcggcgggcc tcgttcagcg
    13681 cctcgatcac gttcgacgag ttgccgctcg ttgagagcgc gagcagcacg tcgccctcgc
    13741 ggccgtaggc gatcacctgg cgcgcgaaga tcgcgtcggt gccgatgtcg ttcgcgagcg
    13801 cggtgaggat cgccgcgtcc tcggtgaggt cgatcgcggg tcgagccggc cagtggaacg
    13861 gtgcggaacg gaagtccgcg accgcgtcca tcgcatcggt ggccgagccg ccgttgccga
    13921 gcgcgagcag cttgccgctc gcgtcgaacg agcgccggag gactgcggcg gctgcggcca
    13981 gcgcggaagc gccctcggtg agggtctgca cccgcagcgc gcccacctcc tccgccttca
    14041 tgcgtaccga gcgcgccacg tcggcaacca ccgcatcgag gtcgtgctcc tgctcgccta
    14101 agaacggata caggaagctc gacgcgccgg ttccctccat gccacggtcg aggtggtcga
    14161 agaacacgtg cacgagctcc cagagcacgt ggtagagcgt ctcgatcagc tcctggcgga
    14221 tgaacgggtc ctgcgtgggg ggcggaaact cgtgctcgaa cgcgaccgtc aggcatccgc
    14281 gctccgccgc cggggccagc gctgcgacgt cctcaccgaa gccgacgacg atgtcgtccg
    14341 gctcggccag gagctcgacc tgcggcgcca gcggcccgcc ctcgagcgag agcccgatcg
    14401 cgggcagcgc tcgcttcccg acgatcaccg ggtgcacgaa ctcgaccgcg atgtgacgga
    14461 cgtccgagcg cgcggcgggc gtggcgccga tcgcgatcag gcgcccgccg cgcgcgaagc
    14521 gctcggccat ctggcggcac agctcggcga tacgcggggc ctcagcctcg aagaagcgag
    14581 cgttcgcctc ggtgcgctcg cttggcgccg cgaccgcgtc gctcaaacgc gcgcctcctc
    14641 tcgcgcggcc agctgcacga gccagtcgcg gaactgctcc accccctcgc ccgtgcgcgc
    14701 gctcgtgagg atgcggcgcg agtcggggtt caccttgtcg aggttgcaga ggaagcgctc
    14761 gacgtcgaag tcgaggtgcg gcagcaggtc gatcttgttg atcagcacca gctcgcaggt
    14821 gcggaacatg agcgggtact tgagcggctt gtcctcgccc tcggtgatcg acgagaccat
    14881 cacgcgcgca tcctcgccca cgcggaactc cgcagggcac acgaggttcc ccacgttctc
    14941 gatcacgagc aggtcgagct ccggtagcgg gatctccggc agcgcggagc gaaccatgtt
    15001 cgcgtcgagg tggcactcgc cgccgaagcc cgggtccgtg ttcagctgcg tgaccgggac
    15061 gtggagccag gccaggcggt cggcatcgag gctgccctga acgtcgccct cgagcacgcc
    15121 cgcgcgcacg tcaccgacgt cgccgagcac gcgctcgagg agcgaggtct tgcctgcacc
    15181 cggcgcgctc atcaggttca cgaccttcac gcttgcgcga tctaagtcgg agcggttcgc
    15241 ccgcgcaatc gtgttgttgg cgtcgagagc gtcttcga
//
LOCUS       DASVXS010000175         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1178531, whole genome shotgun sequence.
ACCESSION   DASVXS010000175 DASVXS010000000
VERSION     DASVXS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178531"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VF752_09780"
     CDS             <1..594
                     /locus_tag="VF752_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8855880.1"
                     /translation="RDPDRSSRGWPADRGLDRVAMSLTPPVDPERDHILGSVDAPVTL
                     VEYGDFECPYCNDAYPDVKRALARVGPQVRFVFRQLPLFEKHPHAELAAEAAEAAGAQ
                     GRFWDMHDRLFESGRRLDRAHIERYAEELGLDLEQFRSDLDTHRFREQVLEQRQSAID
                     SGAQGTPTFFVDGEPYTGFYDENALSDALLARIARSA"
     gene            700..1554
                     /locus_tag="VF752_09785"
     CDS             700..1554
                     /locus_tag="VF752_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8855881.1"
                     /translation="MPRVTVGSENSAPIEIHYEDHGSGSPIVLIHGYPLDGNSWEKQE
                     RVLLANGYRVIRYDRRGFGRSSQPTVGYDYDTFAADLNTLLDHLDLDDIVLAGFSMGT
                     GEVTRYVGRYGSGRVNKAALFGAIPPFLLKTDDNPEGVDGQVFDDIKAAVVKDRYAYF
                     KDFYDNFYNVDKLAPERISDQAWRASFNVAAAASPYASYACVDAWLEDFRDDVAKIDV
                     PTLLVHGTEDRILPINATADRLPDLIADLQYVRVEGGPHNIAWTHPDEVNEALLSFLA
                     GDRAAAAV"
     gene            complement(1620..2279)
                     /locus_tag="VF752_09790"
     CDS             complement(1620..2279)
                     /locus_tag="VF752_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8855882.1"
                     /translation="MNREEESGAERITIVLADDHVVVRSGLRMLLESEPGLEVVAEAG
                     DVPETLRKVGAYRPRVLILDLNMPGQPSLEAIPELLETSPETRIVVLTMQSDPGFARE
                     ALRAGVLGYVLKEAADDELVEAVRRAADDGTYLNPSLGARLAAEPAQPQGPPDNLSER
                     EAEVLGLIALGHTNNEIAERLYLSVRTVESHRAHIQQKLGLTTRAELVRYALDHGLVE
                     T"
     gene            2378..2824
                     /locus_tag="VF752_09795"
     CDS             2378..2824
                     /locus_tag="VF752_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8855883.1"
                     /translation="MPITLPRNSARTTDPLLGGDPYDIQVRELMTPGVVTIAEDASLA
                     RVYGAIAAHGVHAVLVLGIQTGRPLGWVTARGLLAWMDSDHALVSARDAITEPIRTIE
                     PAATARDAIRELSQPGTTHLLVARSEHALPEGVVSSLNLMSLVRSS"
     gene            2821..>3326
                     /locus_tag="VF752_09800"
     CDS             2821..>3326
                     /locus_tag="VF752_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855884.1"
                     /translation="MSDFHAVICGGGVASAEALLRLRRLAGDSLRTTLIAPNDELIYR
                     PLAVEEPFAKPGIRRYPLEKLVKDTGAEWVRDALDHVDLEARSVSSAGGQHVEFDALL
                     LAVGARLSKPFPYSDVFTDAQADDAMHGLLQDIEGGYTRSVAFIQPEGPAWPLPLYEL
                     ALMTSERAR"
BASE COUNT          529 a         1164 c         1112 g          521 t
ORIGIN      
        1 cgtgatccag accgcagcag ccgcggttgg ccggccgacc gggggctcga ccgggtagcg
       61 atgagcctca cgcctcccgt cgaccccgag cgcgaccaca tcctcggctc cgtcgacgca
      121 cccgtgacac tggtcgagta cggggacttc gaatgtccct actgcaacga cgcctacccg
      181 gacgtgaagc gcgcgctcgc gcgggtgggg ccgcaggtgc gcttcgtgtt caggcagctg
      241 ccgctgttcg agaagcaccc gcacgctgag ctggcggctg aggccgccga ggcggccggc
      301 gcacaggggc gcttctggga catgcacgac cggctgttcg aatcaggcag gcggctcgac
      361 cgcgcgcaca tcgagcgcta cgccgaagag ctcgggctcg acttggagca attcaggtcc
      421 gacctggaca cacaccgatt tcgtgagcag gtcctcgagc agcgccagtc ggcgatcgac
      481 agcggcgccc agggcactcc cacgttcttc gtcgatggcg agccctacac gggcttttac
      541 gacgagaacg ccctttccga cgcgcttctg gcgcgcatcg cgcgatccgc ctgaccgacc
      601 catcgtggaa tccgatcgtg cgatcgcgta gcggtatcgc gcggctgcgg cccgcttcat
      661 aggttcgacc tgtcactccc cccaccagaa agggaaccca tgcctcgcgt aaccgtcgga
      721 tccgagaaca gcgcgccgat cgagatccac tacgaggacc acggttcggg atcgcccatc
      781 gtgttgatcc acggctatcc gttggacggc aactcctggg agaagcagga gcgggtgctg
      841 ctggcgaacg gctaccgggt gatccgctac gaccggcgcg ggttcggccg ctcgagccag
      901 cccaccgtcg gctacgacta cgacacgttc gctgccgacc tcaacacgct gctcgaccac
      961 ctcgacttgg acgacatcgt cctcgccggc ttctcgatgg gcacgggcga ggtgacgcgc
     1021 tacgtcggcc gctacggctc cggacgcgtg aacaaggcgg ccctgttcgg cgcgatcccg
     1081 ccgtttctgc tcaagaccga cgacaacccc gagggcgtcg acggccaggt gttcgatgac
     1141 atcaaggcgg ccgtggtcaa ggaccgctac gcctacttca aggacttcta cgacaacttc
     1201 tacaacgtcg acaagctcgc tccggagcgc atcagcgacc aggcgtggcg ggcgagcttc
     1261 aacgtcgccg ccgcggcgtc cccgtacgcc agctacgcct gtgtagacgc ctggctcgag
     1321 gacttccgcg acgacgtggc gaagatcgac gtgcccacgc tgctcgtgca cggcaccgag
     1381 gaccggatcc tgccgatcaa cgcgaccgcc gaccggctgc ccgacctgat cgcggatctc
     1441 cagtacgtgc gggtcgaggg cggcccccac aacatcgcct ggacccaccc cgacgaggtg
     1501 aacgaggcgc tgctctcgtt cctcgccggc gacagagcgg cggccgccgt gtagccggcg
     1561 agacgctggt tcacccggac gaaggggcgg cgcgcacgtt tatgcgcgcc gccttccgtt
     1621 caggtctcca cgagcccgtg gtcgagcgcg tagcgcacga gctccgcgcg ggtcgtgagg
     1681 ccgagcttct gctggatgtg cgcccgatgc gactcgaccg tgcggacgct caggtacagc
     1741 cgctcggcga tctcgttgtt cgtgtgacca agcgcgatca ggccgagcac ctcggcctcg
     1801 cgctcggaga ggttgtccgg tggtccttgg ggctgcgccg gctctgcggc caggcgcgca
     1861 ccgaggctgg ggttcaggta tgtgccgtcg tcggcggcgc ggcgcacggc ctcgacgagc
     1921 tcgtcgtcgg cggcttcctt gagcacgtag ccgagcacgc ccgcgcgcaa cgcctcgcgc
     1981 gcgaagccgg gatcgctctg catcgtgagt accacgatgc gcgtttcggg cgaggtctcc
     2041 agcagctcag gtatcgcctc gagactcggc tggcccggca tgttgaggtc caagatgagt
     2101 actcgcgggc ggtacgcgcc gacctttcga agcgtctctg gcacgtcgcc ggcctcggcc
     2161 accacctcga ggccgggctc ggactcgagc aacatgcgca gaccgctgcg cacgacgacg
     2221 tggtcatcgg cgagcacgat cgtgatccgc tcagcgcctg actcttcctc gcggttcatg
     2281 ggcgtcaacc tagcggtccg cggtccgtag tccccacgga ttgcagaggg ggacccgcgc
     2341 ggatgtccgc ggcgtgcgcc gctcgtacgg tcggggcatg ccgatcacac tgccacgcaa
     2401 ctcagcccgg accacggatc cgctcctcgg gggcgacccg tacgacatcc aggtccgcga
     2461 gctcatgaca cccggcgttg tgaccatcgc cgaggatgcg tcgctggccc gggtgtacgg
     2521 cgcaattgcg gcacatggcg tccacgccgt gctcgtgctc ggcatccaaa ccggcagacc
     2581 tctcggctgg gtcaccgcgc gcggcctgct cgcatggatg gacagcgacc acgcgctcgt
     2641 gagcgcgcgc gacgcgatca cggagccgat tcgaacgatc gagcccgccg ccaccgctcg
     2701 ggacgcgatc cgggagctgt cgcagccggg tacgacgcat ctgctcgtcg cccgttcgga
     2761 gcacgccctt cccgagggag tcgtgagcag cctcaacctg atgtcgctgg tgcgcagctc
     2821 gtgagcgact tccacgcggt catctgcggc ggaggcgtgg catcggcgga ggcgcttctg
     2881 cggctgcgga ggctcgccgg cgacagcctg cgtaccacgc tcatcgcgcc caacgacgag
     2941 ctcatctacc gaccgctcgc cgtcgaggag ccgttcgcga agccgggcat tcgccgctac
     3001 ccgctcgaga aactggtgaa ggacacgggt gccgagtggg tgcgcgatgc cctcgatcac
     3061 gtcgacttgg aagcgcgctc ggtatcgagc gcaggcgggc aacacgtgga gttcgacgcc
     3121 ctgctgctgg cggtcggcgc tcgactctcg aagccgttcc cgtacagcga tgtgttcacc
     3181 gacgcccagg ctgatgacgc catgcacggg ctgctccagg acatcgaagg cggttacacg
     3241 cgcagcgtcg cgttcataca gccggaaggc ccggcgtggc cgctgccgct gtacgagctc
     3301 gcgttgatga cgagcgagcg cgcacg
//
LOCUS       DASVXS010000176        14337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_801384, whole genome shotgun sequence.
ACCESSION   DASVXS010000176 DASVXS010000000
VERSION     DASVXS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801384"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VF752_09805"
     CDS             <1..1182
                     /locus_tag="VF752_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8855885.1"
                     /translation="AGISTSGEVDGESKQAVASTLRQKGLIVLDIEEQAPANAGDLLQ
                     RFKKVKPHDLTVATRQLATMISSGMSLLRALYVLEEQSENEKLKEAFVEVRKDVEAGI
                     SFSDALRRHPDIFNELYVAMVAAGETGGILEGTLERVADQLEKDDSLRRQVKSAMMYP
                     AMIASFAGLVLTALVAFIVPVFEKIFKDFGGSLPTITKFTVAISHFITGRWYVCILLI
                     WGAIYAFRKWKSSKRGREQWDRFKLKIPMKIGGVVQKVALARFSRTYSALVAAGVPML
                     EAIEITGRTSGNKVVEKAMHDVRESVKGGGTLAAPMRNTPEAFPEMVVQMIAVGEETG
                     ALDTMLSKIADFYEDEVAATLKALTSILEPIMIILVGAIVGFIVISMYMPLFKVYDQI
                     K"
     gene            1610..2035
                     /locus_tag="VF752_09810"
     CDS             1610..2035
                     /locus_tag="VF752_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8855886.1"
                     /translation="MPAKGIQHVDLAVTDVERSLAFYLDLLGPLGWAEEARFPTYRGT
                     EEVVYLRDPTAGTMLGIRPADGGEHRYYSVGIEHLAFEVDERDEVDAAYSRCLAQKAN
                     IHFPPEEDRDLADYYALFVFDPDGIRIEVFWWRRTDSGT"
     gene            complement(2039..2467)
                     /locus_tag="VF752_09815"
     CDS             complement(2039..2467)
                     /locus_tag="VF752_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855887.1"
                     /translation="MSQENVQLVRSLYESGLMDRDPEELLELATPDVEYVNPPYAVEP
                     GVRHGLVAVAQAMRRFAEVWEESRHELRELYDGGDVVVAAVSWHIRNRGSQKELVNEE
                     AHTWTLRGGRIARFEWGQDLAKALEAADIVDAPFGGREDR"
     gene            2532..2894
                     /locus_tag="VF752_09820"
     CDS             2532..2894
                     /locus_tag="VF752_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855888.1"
                     /translation="MAGTDNERVLQAKQVLDRLDWYPRRVRLQRVRIVHVPWFFRLPL
                     LRRFDGYTAWNLILFRQPLADVPLGLLAHELTHVWQMQHHPVRMPLSYLRFGYSDNPN
                     ERQARGAAALAEELSPGS"
     gene            3028..3675
                     /locus_tag="VF752_09825"
     CDS             3028..3675
                     /locus_tag="VF752_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8855889.1"
                     /translation="MTPTRTTIAGIAIPDSTLAREATEFVQDASTQLLFDHSRRVFLW
                     GTLRGEKLGLNYDPELLYVGAMFHDIGLVEGHRSEHERFEIDGANAARAFLERHGLPE
                     ESVMTVWESIALHTTPEVPRYKQPEVWMVNLGVEYDVIGLHFDDLTAKQREAVLGAHP
                     RTGFKQGIVEAFSAGMRDKPETTFGTMNADILEATLPGYVRPNFCDLIRNSRFEA"
     gene            complement(3690..4175)
                     /locus_tag="VF752_09830"
     CDS             complement(3690..4175)
                     /locus_tag="VF752_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8855890.1"
                     /translation="MLNGDGRDARDDIALLVLRVPELPLSPQAEVTRLLPSEPDAAAL
                     AREALDELEPMLADDVFATVRLLVSELVTNSVRHAEAGAGQMIELRATLLADRLRVEV
                     TDRGAGFDETRRAPDRHSTSGWGLYIVDQLADSWGVVRDDATRVWFEIERGSGNRPAG
                     A"
     gene            complement(4176..4382)
                     /locus_tag="VF752_09835"
                     /pseudo
     CDS             complement(4176..4382)
                     /locus_tag="VF752_09835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
     gene            complement(4286..5668)
                     /locus_tag="VF752_09840"
     CDS             complement(4286..5668)
                     /locus_tag="VF752_09840"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX8855891.1"
                     /translation="MAAVAAAYYGAAKLGLSLAFMAKSVTAVWPPTGIALAALVLWGY
                     RLWPGVALGALLANSWTGVPFYAVLGITAGNTLEALLGTFLLRRAGFRPSLERVKDVI
                     ALVVLAGVVSTTVSASIGVTSLLLGGEIGADSFGTVWRTWWLGDMGGDVLIAPAILIA
                     ATHWPFKRAPGGRLEAAALALLLGGLCGVVFSQEVGLTYLLFPLLIFATLRFWQPGAA
                     AASLLIASIAIPLTERDIGPFAGHSPDERLLLAQTFVGIACLTGLVLAAVITERKRAE
                     DTVEYIAETLQESLLPARPPEIPGLETAVDFRPAGERHRVGGDFYDLFERDDGSWAVV
                     IGDVCGKGARAAAVTGLARYTLRAAAVDESRPSRILDLLNNAILRQRAPDEYCTVAYA
                     RLELNGAEVARATVSCGGIRCRSCCAPTEPSSRSVASAPCSEWTRSQRWRTTSPNSRP
                     ATPWCFTRTG"
     gene            5854..6231
                     /locus_tag="VF752_09845"
     CDS             5854..6231
                     /locus_tag="VF752_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8855892.1"
                     /translation="MAVDLTIQIENTPGALAQVAAAISDAGVNIAAATCIGLGATAEL
                     HILVPRAEPVKHALAISHLAITHEREVVVVDVEDRPGVLADLTRKIARASVDLDLVYV
                     ATQNRVVFGSPDLAGLKAALGAA"
     gene            6304..7176
                     /locus_tag="VF752_09850"
     CDS             6304..7176
                     /locus_tag="VF752_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8855893.1"
                     /translation="MPGDQIVRANGVDLCVETFGDRADPAILLIHGTGSSMLSWDEEL
                     CERLAARGRFVIRYDSRDAGRSVTYEPGNPPYTLRDLVADAVALLDTFELTRAHILGM
                     SGGAAIGQLLALDHPDRVASLTLASSTPGIPGEENRDLPPVADQLRASFAQEPPMPDW
                     TDRAAVIDYLVEAERPCAARSRPFDEAAARRLAGRVFDRSANIEASVTNPFLVDPGEG
                     WRNRLRQIAAPTLIVHGTEDPMFPFGHALALAKEIPGAELRGLEHTGHEYFPPSTWDL
                     VVPAIERISNRGGP"
     gene            7176..7733
                     /locus_tag="VF752_09855"
     CDS             7176..7733
                     /locus_tag="VF752_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8855894.1"
                     /translation="MRKLIYSFGVSLDGFIAGPGGTIDWGMPDEELHRFHNDEVRASG
                     IQLYGRRLYETMTYWETAEDDPALSEVAREFARIWKATPKLVFSKTLENVEGNSTLVK
                     GDPVEEVAKLKQEPGKDLAVGGAGLASSLVAAGLVDEYRLFVYPVILGAGTPYFPPLD
                     QRINLELLETRTFGSRVVYVRYRTT"
     gene            complement(7730..8209)
                     /locus_tag="VF752_09860"
     CDS             complement(7730..8209)
                     /locus_tag="VF752_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855895.1"
                     /translation="MPFPPRPIDSTLHGATDYTAGATLMTIFPRLAGIEGTRTARQIR
                     RAGAIHAGYSTLTSYPLGIVKVLPFKAHLAIDAIGAIALAATPFLTGQFLKGKKEWVP
                     HVGLCMFELSSLLLTDPTDKGEFHANLEAVREANTEDPHRKIYEGPPAVVRPAAAAA"
     gene            complement(8221..9399)
                     /locus_tag="VF752_09865"
     CDS             complement(8221..9399)
                     /locus_tag="VF752_09865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX8855896.1"
                     /translation="MRANVWSGRNTVQVENVPDPKILNDRDAIVKITSTAICGSDLHL
                     YDGYVPTMKKGDILGHEFMGEVVATGRGVTKLQIGDRVVVPFPIACGDCWSCRHELYS
                     VCENSNPNAGMAEKFFGHPTAGIYGYSHLTGGYAGGQAEYARVPYADVGPIKIEDDIE
                     DEKVLFLSDIFPTGFFGADLCDIQGGEVIAVFGAGPVGQFAIASSILLGAERVIAIDQ
                     YDYRLAMAVNKAGATETINFSENPDIVEQLKEMTGGRGPDAVIDAVGMEAAHGRGPVH
                     AYDRVKQATRSETDRGHALRDAILACRPGGIVSVIGAYGGLIDKFPAGAFMNKGLQMR
                     TGQCHVQKYLQPLYERVRNGEIDPSFVVTHRLELGKAPDGYETFKHKQDECVKVVLKP
                     "
     gene            complement(9399..9788)
                     /locus_tag="VF752_09870"
     CDS             complement(9399..9788)
                     /locus_tag="VF752_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855897.1"
                     /translation="MTAKAAITVLRSREEIQQLWQSSEYRPKYIEGAEAAVTFVDAPG
                     DRGTEIHVDLEHGGQNAKLGEIVQKLTGAAALPKVKDDLRRFKQHVETRVIPRSEGSP
                     EGESAERKFKQRPAQPLEESELQKVGV"
     gene            complement(9871..10281)
                     /locus_tag="VF752_09875"
     CDS             complement(9871..10281)
                     /locus_tag="VF752_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8855898.1"
                     /translation="MLSRAVRLGYAAANRGDFDVVLVGLDPAFEYLPAEGLRPPDLET
                     VFRGHEGYVQLWRYWLDAFGDIHWDPEELFDFGDQMLVTTHQRGHGLGSGVAVSEPVF
                     QLYQLRGGLIVRHEDFLDRSKALDAAASRPPVQH"
     gene            complement(10501..11442)
                     /locus_tag="VF752_09880"
     CDS             complement(10501..11442)
                     /locus_tag="VF752_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8855899.1"
                     /translation="MPEPSVPRFEAWQVDLHGHRAIYRIAGSGPPVVLIHGMVNSSRH
                     WEQVALRLADSYTVIAPDLIGHGDSATPRGDYSLGAHAAVIRDLLTVIGVERATIVGH
                     SLGGGVAMQFFYQFPQRTERLALISSGGLGHEVSPMLRSAALPGMSALLALAAHRRVL
                     DLLWAIGERLRARQSGVGVYVHAVVRALRPLERAGAREAFLQTLRSVIDVRGQRVSAR
                     DRLYLLDSVPTLIVWGGRDHTIPVEHGRATSASIPHAEFVTLPRAAHFPHLEDPEGLA
                     DALRRFLESTEPAPIDDADWGSIIAPRSPRSRIPLER"
     gene            complement(11478..11957)
                     /locus_tag="VF752_09885"
     CDS             complement(11478..11957)
                     /locus_tag="VF752_09885"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8855900.1"
                     /translation="MTRYTLLETPIGRLAVAGNGRTLNAIRLEGRPHPSWVRDDTALA
                     PITAQLRAYFAGELAEFDLELEPQFGGEFERSVWREVATIPYGETASYLEIATRIGKP
                     AAVRAVGRANGRNPIPIVVPCHRVIGSNGSLTGYAGGLETKRRLLDLEAGITPLAVA"
     gene            12012..12854
                     /locus_tag="VF752_09890"
     CDS             12012..12854
                     /locus_tag="VF752_09890"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8855901.1"
                     /translation="MASLLSEPAPLEAVLVRPHGLLEGPRITPAGVVFSDVIAGGVFR
                     VAGDEVSELLPRRHGIGGLVPHRDGGFVVTGRSVLHVRDGAEQRELLTLDGVSGFNDL
                     STAANGDLLVGALRYRPLAGESPRPGILLRLTADGSAEMLSEGVVWPNGVGPSPDGAT
                     VYVSDYADRRVLAIPADGGAAEVFSESPRGSADGLAVDADGGVWVALGEGGGVARFTA
                     AGELEAIVDVPARFVSSLSFGGEDGRDVAITTADNLRTPETGGCVFMARSEVPGLPVV
                     PAAV"
     gene            12879..13241
                     /locus_tag="VF752_09895"
     CDS             12879..13241
                     /locus_tag="VF752_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855902.1"
                     /translation="MGRRQRRREKRGGEAAAVETTAYTDPEGNVLMLRNSVSVGTVQK
                     IRDLDSTPASSAEDRWQRRTELLFERFAVGWEIAGLPLETQNELLGRYRMASGDERRW
                     IRDTIDAHVREHQPELAP"
     gene            13361..13654
                     /locus_tag="VF752_09900"
     CDS             13361..13654
                     /locus_tag="VF752_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855903.1"
                     /translation="MRALAVVLVLLLVVAFVLAIVAWVRRHRQSPARWKLREHSNGDS
                     VRLYAACEGEPPLLIETVPLGGEGFKERLYQARAEARGKVIALNARRRAELRA"
     gene            complement(13663..14055)
                     /locus_tag="VF752_09905"
     CDS             complement(13663..14055)
                     /locus_tag="VF752_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855904.1"
                     /translation="MKRGAILALPLALLAACGGGGKSEKNADGVRATAKSFVSDIADK
                     KFDEACTLLTTAAKAQLSKAGGGECSKQLSLARGLLSDKQLDTLQDQADKMKVTIKGD
                     TATSAPVSGGDTPGKYIWQGEKWLIDAS"
BASE COUNT         2275 a         4811 c         4878 g         2373 t
ORIGIN      
        1 gcgggcatct ccacgagcgg cgaggtcgac ggcgagagca agcaggctgt tgcgtcgacc
       61 cttcgccaga agggcctgat cgtcctcgac atcgaggagc aggctccggc gaacgccggc
      121 gacctcctcc agcgcttcaa gaaggtcaag ccgcacgacc tcacggtcgc cacgcggcag
      181 ctggcgacga tgatttcgtc gggcatgtcg ctcctacgag ctctctacgt gctcgaggag
      241 cagtccgaga acgagaagct gaaggaggcg ttcgtcgagg tccgcaagga cgtggaggcg
      301 ggcatctcct tctcggacgc gctcaggcgc caccccgaca tcttcaacga gctctacgtg
      361 gcgatggtgg ccgcgggcga gaccggcggc atcctggagg gaaccctcga gcgcgtggcc
      421 gatcagctcg agaaggacga ctcgctcagg cgtcaggtga agtcggcgat gatgtacccg
      481 gcgatgatcg cgagcttcgc ggggctcgtg ctcacggccc tggtcgcatt catcgtcccg
      541 gtcttcgaaa agatcttcaa ggacttcggc ggctcgctgc caacgatcac gaagttcacg
      601 gtcgcgatct cgcacttcat tacggggcgc tggtacgtct gcatcctcct gatctggggc
      661 gcgatctacg cgttccgcaa gtggaagtcg tcgaagcgcg gccgcgagca gtgggaccgc
      721 ttcaagctca agatcccgat gaagatcggc ggcgtggtgc agaaggtcgc gctggcgcgc
      781 ttctcccgca cgtactcggc gctcgtggcg gccggcgtgc cgatgctcga ggcgatcgag
      841 atcaccggcc gcacgtcggg caacaaggtc gtcgagaagg caatgcacga cgtgcgcgag
      901 tccgtgaagg gcggcggcac gctcgccgcg ccgatgcgca acacaccaga ggcgttcccc
      961 gagatggtgg tccagatgat cgccgtcggc gaggagacgg gcgcgctcga cacgatgctg
     1021 tcgaagatcg ccgacttcta cgaggacgag gtggccgcca cgctgaaggc gctcacgtcg
     1081 atcctcgagc cgatcatgat catcctcgta ggcgcgatcg tcggcttcat cgtgatctcg
     1141 atgtacatgc cgctgttcaa ggtctacgac cagatcaagt aggcgggctc tcgcaggcca
     1201 gtcgatcggc agcgagccgc agaccgccgg ctgctgaggt tccgactcga actaggtccg
     1261 gcgccagtcc ggggccggga atcccgccgg gcgaactctg gttaccgagc tgctgcccac
     1321 gatctcaccc ggactgtcct cgatcctgac caccgagaac ttacccggcg ggaagtctcc
     1381 gaggaggtcg agcatcctga gcgcggatcc cgcagactca agttctcgga ggatcgcgcc
     1441 gtcgctatct cgcaccagca ggtactgcgc catgccaaat gggagccggg gagcggattc
     1501 ccggttttgg tacccgggct cagctcattt gacccgtgca tcgcactcag ctcttctttt
     1561 gggcgtggcc gggcgatagg gtcggacgaa ggcagcctcc ggaggagtcg tgccagcaaa
     1621 gggcatccaa cacgtcgatc tcgcggtcac cgatgtcgag cgatcgcttg cgttctatct
     1681 cgaccttctc gggccgctcg gttgggcgga ggaggcccgc ttcccgacct accgaggaac
     1741 cgaggaagtc gtgtacctgc gggatccgac cgccgggacg atgcttggga ttcggccggc
     1801 cgacggcggt gagcaccgct attactccgt gggaatcgag caccttgcgt tcgaggtaga
     1861 cgagcgagat gaggttgacg ccgcgtactc gcgatgcctc gcgcagaagg cgaacattca
     1921 ctttcctccc gaagaggatc gcgatctcgc ggactactac gcattgttcg tcttcgaccc
     1981 ggacgggatt cggatcgagg tcttctggtg gcggcgcacg gatagcggga cctagcgtct
     2041 atcggtcttc gcgaccaccg aaaggcgcat ccacgatgtc cgcggcctcg agcgccttcg
     2101 cgagatcctg tccccactcg aaccgcgcga tcctgccccc gcgcagggtc caggtgtgcg
     2161 cctcttcgtt gacgagttcc ttctgacttc cgcgattacg gatgtgccag ctcacggcgg
     2221 caacgaccac atccccaccg tcgtacagct cgcggagctc gtgccgggat tcctcccaga
     2281 cttcagcgaa gcggcgcatg gcctgcgcga cggcgacgag accgtggcgc acgccgggtt
     2341 cgacggcgta gggagggttg acgtactcga cgtcgggagt cgcgagttcg aggagctcct
     2401 caggatctcg atccatcagt cccgactcgt agaggctgcg gaccagctga acgttctcct
     2461 gcgacacagg ccagtcagta tctcgccgcg ggcggagtac cttcaacagc ggttcggcgg
     2521 cgttggcgac catggcgggc acagacaacg agcgggtcct tcaggccaag caggtgcttg
     2581 accgcctcga ctggtatccg cggcgtgtcc ggctccagcg cgtacgcatc gtgcacgttc
     2641 cgtggttctt ccgcttgccg ctcctgcgtc gcttcgacgg ctacaccgcc tggaacttga
     2701 tcctgtttcg gcagccgctc gccgacgtgc cgctggggtt gcttgcccac gagctcaccc
     2761 acgtatggca gatgcaacac catccggtgc gcatgcctct ctcctacctg cgcttcggtt
     2821 actcggacaa cccgaacgag cggcaggcgc ggggtgcggc ggcgctggcc gaggagctgt
     2881 ctcccggcag ctagctcaag gcagcgccag agaaagaccc cggggatctc cacctcgccg
     2941 cgcccgatta tcgggatcga tcgcgatgcc cgatcaccgg gccgggcgca cttgcccgct
     3001 acctcgaggc ggtccattct gagcagcatg acccccacca gaacgacgat cgccggtatc
     3061 gcgatcccag acagcacgct cgcccgcgag gcgaccgagt tcgtgcaaga cgcttcgacg
     3121 cagcttctgt tcgaccactc ccggcgcgtg ttcctgtggg gcacattgcg gggtgagaag
     3181 ctcggcctga actacgaccc cgagttgctc tatgtcggag ccatgttcca cgacatcggg
     3241 ctcgtggagg ggcatcgctc cgagcacgag cgtttcgaga tcgacggagc gaatgcggcg
     3301 cgcgccttcc tcgagcggca cggcctgccc gaggagagcg tgatgaccgt ctgggagtcg
     3361 atcgccctgc acaccacgcc cgaggtcccg cgctacaagc aaccggaggt ctggatggtg
     3421 aaccttggcg tggagtacga cgtgatcggt ctgcacttcg acgatctcac cgcgaagcag
     3481 cgcgaggcgg tcctgggcgc gcatccgcgc accggcttca agcagggcat cgtcgaggcg
     3541 ttctctgccg gcatgcgcga caagcccgag accacgttcg ggacgatgaa cgccgacatc
     3601 ctcgaggcga cgctgcccgg ttacgtgcgg cccaacttct gcgacctcat ccgcaactcg
     3661 cgcttcgagg catagcgccg ctctgctggc taagccccgg ctggcctgtt cccggacccg
     3721 cgctcgatct cgaaccagac gcgcgtggcg tcgtctctca ccacgcccca gctgtcagcg
     3781 agctggtcga cgatgtaaag cccccagcct gacgtgctgt gtcggtcggg ggctcgccgc
     3841 gtttcgtcga agcctgcgcc gcggtcggtc acctccacgc gcaggcgatc cgcaagaagc
     3901 gtcgcgcgga gttcgatcat ctgccccgca ccggcttcag cgtggcggac gctgttcgtg
     3961 acgagctcgc ttacaagcaa gcggacggtg gcgaacacgt cgtccgcgag catcggctcg
     4021 agttcgtcga gcgcttctct tgccagcgct gcggcgtctg gttcgctcgg aagcagtctg
     4081 gtcacttcgg cttgagggct gagtgggagc tctgggacgc ggaggaccag caaggcgatg
     4141 tcgtcccgcg cgtccctccc gtcaccgttt agcaccgctt gccggatgcc ctgagcgatc
     4201 tcgggcgcgg tctggccggc gcatgactgc aggattgaca tcaggtccgc gctcgtgatc
     4261 actcgggcgg gcgcgtaggc gtcggtcagc ccgtccgtgt aaagcaccag ggcgtcgccg
     4321 gccgcgagtt cggcgacgtc gtccgccaac gctggcttct cgtccattcc gagcagggtg
     4381 ccgaagcgac cgagcggctc gacggctccg tcggcgcgca gcacgagcgg cagcggatgc
     4441 ccccgcagga caccgtcgcg cgcgcgacct cggcaccgtt gagctcgagc cgggcgtagg
     4501 cgacggtgca gtattcgtcg ggcgcgcgtt gacgcaggat tgcgttgttc agtaggtcga
     4561 ggatgcggct cgggcgggac tcatcgaccg cagcggctcg cagcgtgtaa cgcgctaggc
     4621 cagtgaccgc ggccgctcgg gcgcctttgc cgcagacatc accaattaca accgcccagc
     4681 ttccgtcatc gcgttcgaac aggtcgtaga agtcgccccc gacgcggtgg cgctccccgg
     4741 cggggcggaa gtcgaccgcc gtctcgagcc cgggaatctc gggcgggcgc gcgggtagca
     4801 ggctctcctg aagcgtctcc gcgatgtact cgacggtgtc ctctgcccgt ttgcgctcgg
     4861 tgatcacggc cgcgagcacc agtccggtga ggcaggcaat gcctacgaag gtctgtgcga
     4921 gcagcagccg ctcgtccggt gagtggcccg cgaagggtcc gatgtcgcgc tcggtcaacg
     4981 ggatcgcgat tgaggcgatc agcagactgg cggcggcggc gcccggttgc cagaaacgca
     5041 aggtcgcgaa gatcaacagc gggaacaaga ggtaggtcag gccgacctct tgggagaaca
     5101 cgacgccgca gagcccgccc aggagcagcg cgagcgcggc cgcctcgagt cggccgcccg
     5161 gggcacgctt gaagggccag tgcgtcgccg cgatcagtat cgccggcgcg atcaacacgt
     5221 cgccgcccat gtctcccagc caccaggtgc gccagacggt cccaaagcta tccgcgccga
     5281 tctcacctcc gagcagcaga ctggtgacgc cgatgctggc gctcacggtg gtgcttacta
     5341 cgcccgccag aacgacgagc gcgatcacgt ctttaacgcg ctcgagcgac ggtcggaagc
     5401 cggcgcgacg caggaggaac gtcccgagca gcgcctcgag ggtgttcccc gctgtgatcc
     5461 cgagcaccgc gtagaagggc actccggtcc agctgttggc cagcagggcg ccgagcgcga
     5521 cccctggcca gagcctgtag ccccagagga ccagggctgc caaggcgatc ccggtcggtg
     5581 gccacaccgc cgtgacgctc ttggccatga aggccaagct caggcccagc ttcgcggcgc
     5641 cgtaataggc cgccgcaacg gcggccacct caaccgcgta gagcttcgag taacgaccca
     5701 aactgactca ctcgatgccg agccgtaagg aacctactcc agaacgggta cagacccaca
     5761 gccctatacc ggtcacagtc gcaaactccg agatagctag agcgcgggaa tcccggccat
     5821 gctcgggctg taggctgacc acgcacggca ctcatggcag tcgacctgac gatccagatc
     5881 gagaacactc ccggggcgct ggctcaggtc gccgctgcca tcagcgacgc aggcgtcaac
     5941 attgcggcgg ccacgtgcat cggtctcggc gcgaccgccg agctgcacat cctcgtccca
     6001 cgcgccgagc ccgtgaagca tgcgctcgcg atctctcatc tcgccatcac gcacgagcgc
     6061 gaggtcgtgg tcgtcgatgt cgaagaccgc cccggcgtgc tcgccgatct cacacgcaag
     6121 atcgctcgcg caagcgtcga tctcgacctc gtctacgtcg cgactcagaa ccgggtggtg
     6181 ttcggctcgc cggatctggc gggcctcaag gccgcgctcg gcgcggcctg acgagacgcg
     6241 cgcgggatct cggcgggggc cggcgattag ttttcgcgcg cgcatcggtc agagcaagtg
     6301 catatgccag gggaccaaat cgtgcgggcg aacggcgtcg atctctgcgt cgagaccttc
     6361 ggcgatcgcg ctgacccggc gatcctgctg atccatggga cgggcagctc gatgctgtcg
     6421 tgggacgagg agctctgcga gcggctggcg gcgcgcgggc gcttcgtgat tcggtacgac
     6481 agccgcgacg ccggccgctc cgtgacttac gagcctggca atccgccgta cacgctgcgc
     6541 gacctcgtcg cagacgccgt cgcgctgctc gacacgtttg agctgacgcg ggcccacatc
     6601 ttgggcatgt cgggcggcgc tgcgatcggg cagctgctcg cgctcgacca tcccgaccgg
     6661 gtcgcgtcgc tcaccttggc ctcgtcaacg ccgggcatcc ccggcgagga gaatcgcgac
     6721 cttccgcccg tcgcggatca gctcagggcg tcgttcgcgc aagagccgcc gatgcccgac
     6781 tggaccgatc gggcggccgt gatcgactac ctggtcgaag ccgagcgtcc gtgcgctgcg
     6841 cgctcgcgcc ccttcgacga ggcggcggcg cgcaggctgg ccggacgcgt cttcgaccgc
     6901 agcgcgaaca tcgaggcgag cgtgacgaac ccgttcctcg tcgatcccgg cgaaggctgg
     6961 cggaacaggc tgcggcagat cgccgctccg acgctcatcg tgcacggcac cgaggaccca
     7021 atgttcccgt tcggccatgc gctggctctc gcgaaggaga tcccgggcgc cgagctccgc
     7081 ggtctggagc acacggggca cgagtacttc ccaccatcaa cctgggatct cgtcgttccc
     7141 gccatcgaac gcatttccaa ccgaggagga ccttgatgag gaagctgatc tactcattcg
     7201 gcgtgtcgct ggacggcttc atcgccgggc cgggcggcac gatcgactgg gggatgcctg
     7261 acgaggagct gcatcgcttc cacaacgacg aggtgcgcgc gagcggtata cagctctacg
     7321 ggcgccggct gtacgagacg atgacgtact gggagacggc cgaggacgat cccgccctgt
     7381 ctgaggtcgc gcgcgagttc gcgcggatct ggaaggcgac gccaaagctc gtgttctcga
     7441 agacgctcga gaacgtcgag gggaactcca cgctcgtcaa gggcgacccc gtggaggagg
     7501 tcgcgaagtt gaagcaagaa cccgggaagg atctagcggt cggcggagcc gggcttgcct
     7561 cgagcctggt tgccgcgggc ctggtcgacg aataccggct gttcgtgtac ccggtgatcc
     7621 tgggcgccgg gacgccctac ttcccgccgc tcgaccaacg catcaacctc gagctgctcg
     7681 agacccggac gttcgggtca cgcgtcgttt acgtccgcta tcggactact taggcagcag
     7741 ccgcggccgg ccgaacgacc gccggtggac cctcgtagat cttccggtgc gggtcctcgg
     7801 tgttcgcctc gcgtacggcc tcgagattgg cgtggaattc gcccttgtcg gtcgggtcgg
     7861 tcagcagcag cgagctgagc tcgaacatgc agaggcccac gtgcggcacc cactccttct
     7921 tgcccttcag gaactgcccg gtcaggaacg gcgtcgccgc gagcgcgatc gctccaatcg
     7981 cgtcgatggc caggtgtgcc ttgaacggca gcaccttcac gatcccgagc ggatagctcg
     8041 tgagcgtgct gtagccagcg tggattgccc cggcccgccg gatctggcga gcggtgcgcg
     8101 ttccctcgat gccggccagt ctggggaaga tcgtcatcag ggtggcgccg gccgtgtagt
     8161 cggtggctcc gtgcagcgtg gagtcgatcg gccttggcgg aaatggcatc gctggtcctt
     8221 tcagggcttg aggacgacct tcacgcactc gtcttgcttg tgcttgaagg tctcgtagcc
     8281 gtcgggtgcc ttgccgagct cgagccggtg cgtgacgacg aagctcgggt cgatctcgcc
     8341 gttccggacg cgctcgtaga gcggctgcag gtacttctgg acgtgacact ggccggtccg
     8401 catctgcagc cccttgttca tgaacgcccc ggcggggaac ttgtcgatga ggccgccgta
     8461 ggcgccgatc acagacacga tgccgcccgg gcggcaggcg aggatcgcgt cgcgcagcgc
     8521 gtgaccgcgg tcggtctccg atcgggtcgc ctgcttcacg cggtcgtacg cgtgcactgg
     8581 gccgcggccg tgtgcggcct ccatgccgac ggcgtcgatc accgcgtccg gcccgcgacc
     8641 gcccgtcatc tccttgagct gctcgacgat gtcggggttc tcggagaagt tgatcgtctc
     8701 cgtggcgccc gccttgttca ccgccatcgc cagccggtag tcgtactgat cgatcgcgat
     8761 cacgcgctcg gcgccgagca ggatcgagct cgcaatcgcg aactggccga ccggcccggc
     8821 accgaagacc gcgatcacct caccgccctg tatgtcgcag agatcggcgc cgaagaagcc
     8881 ggtcgggaag atgtccgaga ggaacaggac cttctcgtcc tcgatgtcgt cctcgatctt
     8941 gatcgggccg acgtcggcgt acggcacgcg cgcatactcg gcctggccgc cggcgtagcc
     9001 gccggtcagg tgcgagtagc cgtagatccc agccgtgggg tgaccgaaga acttctcggc
     9061 catgcccgcg ttcgggttcg agttctcgca gaccgaatag agctcgtgcc tgcacgacca
     9121 gcaatcgcca caggcgatcg ggaacgggac caccacgcga tcgccgattt ggagcttcgt
     9181 aacgccgcgc ccggtcgcga cgacttcgcc catgaactcg tgccccagga tgtcgccctt
     9241 cttcatcgtc ggcacataac cgtcgtagag gtggaggtcg gagccgcaga tggccgtcga
     9301 cgtgatcttc acgattgcgt cgcggtcgtt gaggatcttc gggtccggga cattctcgac
     9361 ctgaacggtg ttccgtccgg accagacgtt cgcgcgcatc acacacccac cttctggagc
     9421 tcggactctt cgagcggctg tgccggccgc tgcttgaact tgcgctcggc cgactcgccc
     9481 tccggcgagc cctcggaccg cgggatgacg cgagtctcca cgtgctgctt gaaccgccgc
     9541 aggtcgtcct tcaccttcgg cagcgccgcg gcgccggtca gcttctgaac gatctccccg
     9601 agcttcgcgt tctggccgcc gtgctcgagg tccacatgga tctcggtgcc acgatctccc
     9661 ggcgcgtcga cgaacgtgac cgccgcttcg gctccctcga tgtacttcgg gcggtattcc
     9721 gagctctgcc acagctgctg gatctcctct cgggagcgga gcacggtgat cgctgccttg
     9781 gcagtcatgc agggggcctc cttgggacgc gggcgttcga gggcaactgc ccaggcggct
     9841 tcccgccaaa cctccaactg ggggctacgc ctagtgctga accggcggcc gtgacgcggc
     9901 ggcgtcgagt gccttcgagc ggtcgaggaa gtcctcgtgc cggacaatca acccaccgcg
     9961 cagctggtac agctggaaca ccggctcgct cacagctaca ccgctgccga ggccgtggcc
    10021 gcgctggtga gtcgtaacga gcatctggtc tccgaagtcg aagagttcct cgggatccca
    10081 gtggatgtcc ccgaatgcgt cgagccagta gcgccagagc tgcacatagc cctcgtgtcc
    10141 gcggaagacc gtctcgaggt cggggggcct gaggccctcg gcgggcagat actcgaacgc
    10201 ggggtcgagg ccgaccagga cgacgtcgaa atcgccgcgg ttggcggcgg catagccgag
    10261 gcggacggct cgcgagagca ttgcctgccg gatacgggag cgtggcggaa ggcgctggac
    10321 cgtgtccgcg acgcgacggt acaggcccgg aaaccggaga gacagccgct gatccggcgt
    10381 cctgcgagtg agcgctcgtt caggcagcgg agccagttcg attcggctcc cgcgcaacac
    10441 cggggccgtc tgctgcgccg tgcccacgtg cccagtatct cgctgggctc aaggcggtcg
    10501 ctagcgctcg agcgggatgc ggctgcgcgg tgagcgcggc gcgatgatcg agccccagtc
    10561 ggcgtcgtcg atcggcgccg gctcggtcga ctcgaggaag cgccgaagcg cgtcagcgag
    10621 gccctcggga tcctcgaggt gggggaagtg cgcggcgcgc gggagggtca cgaactcggc
    10681 gtgggggatg gatgcgctgg tggcgcgtcc gtgctcgacc gggatcgtgt ggtcgcgccc
    10741 gccccagacg atcagcgtcg gcacgctgtc cagcaggtag agccggtctc gcgcactcac
    10801 gcgctggccc cgaacgtcga tgaccgagcg caacgtctgc aggaaggcct cgcgcgcacc
    10861 cgcgcgctcc agcgggcgca gcgcgcgcac cactgcgtgt acgtacacgc cgacgccgga
    10921 ttgacgcgcc cggagccgct ccccgatcgc ccatagcagg tcgagcacgc gccggtgcgc
    10981 ggcgagcgcg agcagggcgg acatgcccgg gagcgcggcg ctccgcagca tggggctgac
    11041 ttcgtggccg agcccgccgc tcgatatgag cgccaggcgc tcggtgcgtt gggggaactg
    11101 gtagaagaac tgcatcgcca cgccgccgcc gagcgagtgc ccgacgatgg tcgcccgctc
    11161 gacgccgatc acggtgagga gatcgcggat gaccgcggcg tgggcgccga gcgagtagtc
    11221 gccacgtggt gtggccgagt cgccgtggcc gatgaggtcg ggcgcgataa cggtgtagct
    11281 gtcggcgagg cgcagcgcca cctgctccca atggcgcgag ctgttgacca tcccgtggat
    11341 gagcaccacg ggtgggccgc tgccggcgat cctgtagatc gccctgtggc cgtgcaggtc
    11401 cacctgccag gcctcgaagc gcggcaccga cggctcgggc atcgcacgag cctatcggcg
    11461 cgcgccgcgc cgcggcgcta agcgaccgcg agcggcgtga tgcccgcctc gagatcgagc
    11521 aggcggcgct tcgtctcgag gccgccggcg tagccggtca gcgagccgtt cgagccgatc
    11581 acgcggtggc acggcaccac gatcgggatc gggtttcgcc cgttcgcgcg accgaccgca
    11641 cgcaccgcgg cgggcttgcc gatacgcgtg gcgatctcca ggtagctcgc cgtttctccg
    11701 tacggaatcg tcgcgacttc tcgccatacg cttcgctcga actccccgcc gaactgcggt
    11761 tcgagctcaa ggtcgaattc cgcgagctcg ccggcgaagt aggcgcggag ctgggcggtg
    11821 atgggggcga gggctgtgtc gtcgcgcacc cagctcgggt ggggccggcc ctcgagccgg
    11881 atggcgttca gcgttcggcc gttgcctgcg acggcgagcc ggccgatagg tgtctcgaga
    11941 agcgtgtacc tggtcatgtg agtggaacgc gccggcgccc gacttcgtga ggcgcgagga
    12001 tgcagcagcg aatggccagc ctactgtccg aaccggcgcc actcgaagcc gtgctcgttc
    12061 gcccgcacgg gcttctcgaa gggccgcgca tcactcccgc gggcgtggtc ttcagcgacg
    12121 tcatcgcagg cggcgtcttt cgcgtcgccg gcgacgaagt gagcgagctc ttgccacgac
    12181 gtcacgggat cggcggcctc gtaccgcacc gcgacggcgg cttcgtcgtc actggacgca
    12241 gcgtgcttca cgtgcgcgac ggcgccgagc agcgcgaact cctcacgctc gacggcgttt
    12301 ccgggttcaa cgacctctcg accgccgcga acggcgacct gctcgtcggc gctctccgtt
    12361 accgcccact ggccggcgag tcgccgcggc cggggatcct gcttcggctc accgccgatg
    12421 ggtcggccga gatgctcagc gagggcgttg tgtggccgaa cggcgtaggc ccgtcgcccg
    12481 acggcgccac ggtctacgtc tccgactacg ccgatcggcg cgtgctcgcc attccggccg
    12541 acggaggtgc cgccgaagtc ttctccgaat caccgcgtgg gtcggccgac ggactcgctg
    12601 tcgacgccga cggcggagtg tgggtcgcgc tcggcgaggg tgggggagtg gcgcgcttca
    12661 ctgcagccgg agagctcgaa gcgatcgtcg acgtgccggc gcgcttcgtc tcgagtctgt
    12721 ccttcggcgg cgaggacgga cgagacgtcg caatcacgac cgcggacaac ctgcggacgc
    12781 ccgagacggg cggctgcgtt ttcatggcac gcagcgaggt gccaggactg ccggtggtgc
    12841 ccgccgccgt gtgaacgtcg cgctagcctc ggcggccgat gggacgcagg cagcgacgga
    12901 gggaaaagcg cggcggcgag gcggccgccg tcgagacgac cgcctacacg gatcccgagg
    12961 gcaacgtttt gatgctccgc aacagcgtct cggtcggcac tgtccagaag atccgagacc
    13021 tcgattcaac gcccgcgtcg tcagctgagg accgctggca gcggcgcact gagctgctgt
    13081 tcgagcggtt cgccgtcggt tgggagatcg ccggcctgcc gctcgagact cagaacgagc
    13141 tgctcgggcg ttaccgcatg gctagcggcg acgagcggcg ctggatccgc gacaccatcg
    13201 acgcgcacgt gcgcgagcac cagcctgagc tggctccgta agagcggcgt ttgtcaaaac
    13261 cccctgagcg ggcagaatgc ttctcgcatt cgacgcgcac caatgggaag gggcacatgg
    13321 atctgatcga gcggagtcgc ggtttgctgc cgatgcggtc gtgagagctc tcgccgtcgt
    13381 actcgtcctt ctactggtcg tcgctttcgt cctcgcgatc gtggcgtggg tacggaggca
    13441 tcgccagtca cctgcaaggt ggaaactgcg cgagcactcc aacggtgaca gcgtccgcct
    13501 ctacgccgcc tgcgagggtg agccgccgct gctgatcgag acggtgccct tgggcggcga
    13561 gggtttcaag gagcgcctat accaagcccg ggcggaagcg cgcggcaagg tcatcgcatt
    13621 gaacgccagg cgccgcgccg agctgcgcgc ctgagcctcg agtcagctgg cgtcaatcag
    13681 ccacttttcg ccctgccaga tgtacttccc gggcgtgtcg ccaccgctga ccggcgccga
    13741 ggtggccgta tcgcctttga tcgtcacctt catcttgtcg gcctggtcct gcagcgtgtc
    13801 gagctgcttg tcgctgagca ggccgcgcgc gagcgacagc tgcttcgagc actcgccgcc
    13861 gccggctttc gacagctgag ccttcgccgc ggtcgtgagc agggtgcagg cctcgtcgaa
    13921 cttcttgtcc gcgatgtcgc tcacgaaaga cttggccgtc gcgcgcactc cgtcggcgtt
    13981 cttctcggac ttgcccccgc cgccacaggc cgccagcagc gccagcggca gcgcgagaat
    14041 cgctcctcgc ttcatcgacc ctcccgtggt cgccgatctc gcgggtgatg cccgcccgcg
    14101 cctgagagaa accaccgtgg gcgcgaggct cgcggtcggt cacgtctcaa gtcggttacg
    14161 cgcgtatccg actagaggga tgtgcgccat ggcgtgcgtc agctcttgcc atgcctcctc
    14221 gtgctggtcg cgggggtggt gatcgcctgg cctgccgagg ttgggctcga ctacgacaac
    14281 gacgccggcc aggcgatcaa cgcgctcgcg cgtggcgatc tcgcgacctt cggcgca
//
LOCUS       DASVXS010000177         9086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1179018, whole genome shotgun sequence.
ACCESSION   DASVXS010000177 DASVXS010000000
VERSION     DASVXS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179018"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VF752_09910"
     CDS             complement(<1..816)
                     /locus_tag="VF752_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8855905.1"
                     /translation="MATKPEKPTIAPIVGPDDPRHFTDSGIEIERVYEEGDVAPGLEE
                     RLGEPGEYPFVRGIHPDMYRSRKWTMRQYAGYATAQETNERFHYLIEHGSTGLSMAFD
                     LPTQLGRDSDDPLCLGEVGRTGVAIDTIDDMRRVFDGIPLAEVSTSMTINAPAAVLLL
                     LYELVGEEQGVPAEELRGTTQNDILKEYIARGNFIYPPVPAMRLTTDLFAYTKERIPK
                     WNTISISGYHMREKGCSAVQEVGFTLANGIAYVQAALDAGLGVDEFAPRLAFFF"
     gene            complement(841..1881)
                     /gene="aroF"
                     /locus_tag="VF752_09915"
     CDS             complement(841..1881)
                     /gene="aroF"
                     /locus_tag="VF752_09915"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX8855906.1"
                     /translation="MIHGDRMMVVMTPQASQAEIAAVVERLEAVGVHAKVMPGELTTA
                     IGAIGDPEGVRALALEGMPGVDQVIPISRPYKLASSELSHHEPTSFSVDGRVVGGNAT
                     FCLIAGPCTVESREQTLAVADAMQAGGASMMRGGAFKPRTSPFSFQGLGPPALDILAE
                     ARERTGLPVVSELTDINWAEPVAEVVDVIQVGARNMQNYALLEVVGKLGKPVLLKRGL
                     SSTVEELLMAADYILKEGNDRVILCERGIRTFETATRFTLDLGAVPYLKLHTHLPVIV
                     DPSHAAGDRKLVEPLSRAAAAIGADGIIVEVAEDPEAALCDGPQQLYARDFAEFAERV
                     AAHAALCGRTLA"
     gene            2010..2999
                     /locus_tag="VF752_09920"
     CDS             2010..2999
                     /locus_tag="VF752_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8855907.1"
                     /translation="MATDGSAAAELTFRDVTKRYAGRAQPAVDHLSLRIPAGEICVLV
                     GPSGAGKTTALKMVNRLIQFDSGDIEIDGRSVLDFEISELRRGIGYVIQQVGLFPHMN
                     VEANVATVPRLRGWGKQRTHERVRELLELVGLDPDGDPRRYPGQLSGGQRQRVGLARA
                     MAADPPLMLMDEPFGAIDPITRSRLQDEFLRLHRQIRKTVIFVTHDIDEAIKMGDRIA
                     ILRQGGTLAQYDTPDAILASPADDFVAQFVGADRALKRLSLVRLSDLELEAVPSQVNG
                     LPSAPRDASLHDALSLLLSEGGSRLLVTDPDGTPLGVARLEQIGARVSEDQAS"
     gene            2996..3733
                     /locus_tag="VF752_09925"
     CDS             2996..3733
                     /locus_tag="VF752_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8855908.1"
                     /translation="MTTLIAQSGPVIPNFGGGGSNSCVRENRTFCWDWFTSHWSSNFQ
                     PHLVEHIVLTAIAVSVGFVIAMVAALFAYRRNWFEQPFTIATGILYTIPSLALFQILV
                     PITGLTRLTAEIALVSYTLLIIFRNILTGLREVPEDVREAARGMGLTRNQILWRVELP
                     IAVPAIVAGVRIATVTVISLATVAAIVGVNQGLGVPILDAIPTTFNTEFIAAAVLTIA
                     LALVADALLVGAQRVLTPWARGRAAYR"
     gene            3730..4437
                     /locus_tag="VF752_09930"
     CDS             3730..4437
                     /locus_tag="VF752_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8855909.1"
                     /translation="MNDFVDAFKFMGDNLSLLLNRTVDHLLLSGAAIGVSLLIALPLG
                     FWLGHLHRASFLAINVSNIGRALPSLAVIAIGISIFGLGFLNSMVALVVLAVPIILTN
                     AYVAVDGVDDDVVEAARGMGMRPVQVLGRVELSLAIPLTFAGIRTAVVYVIATATLAA
                     IAGSGGLGEIIYNQASYGLPGVLAASFWVAALALLADLLLAALQRAVIPRGLRPVRHA
                     DAAVAPPDALRSSEVAA"
     gene            4640..5440
                     /locus_tag="VF752_09935"
     CDS             4640..5440
                     /locus_tag="VF752_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8855910.1"
                     /translation="MTIGSKNFTEQFILGQLYAQALKAKGYTVSLKQNIGSSEIIDKA
                     LTSDKIDMYPEYVGIILTAVAHETAKPKSADDAYQQAKKFEEGRGFTVLDKTPFSNVD
                     AMGVTKAFAQKNGLTTVTDLKKLSSFRLGGPPEFKTRDTGVVGLKKDYGLDNIKFVPL
                     AIGLQYKALDSGKVDAADVFTTDGQLQGGKYTVLKDLENTFGFQNVAPVVSQKVLSKE
                     GTEFSKILNDVSAKLTVEAMRKMNAAVDLDKQEPASVAQQFLSANGLV"
     gene            complement(5437..6591)
                     /locus_tag="VF752_09940"
     CDS             complement(5437..6591)
                     /locus_tag="VF752_09940"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX8855911.1"
                     /translation="MTQPTEQAPRVTTLELFFDLVFVFTITQLTTVLVKDPNGRGVAR
                     VLLMLALIWWMYGGYAWLTNSVTVDRDTRRLLLLGGMAGFLVMALALPTAFGGSGLTF
                     ALGYLVVIVVHGGMFMRASPAGVAQAFSGILPWNITAAALVLIGGAIGGTTQYVLWAV
                     AVALECANPRLTSDEGFVIAPAHFVERHGLVVIVAIGESVVAIGIGAAGLPVDAELVG
                     VALLGLALSACLWWTYFGAGDDVEAEEALSAAPVEARPRLAIDAYFYLHLPILFGIVA
                     VASAIKKATGHAGDPLEFARALALSGGVALFMGADALFRRTLGIGSGAWRGLAALLAF
                     AALPLGTELSAVAQLAALVILLVAVLAAERRHLGARREARRAKPPAAPAV"
     gene            complement(6588..7481)
                     /locus_tag="VF752_09945"
     CDS             complement(6588..7481)
                     /locus_tag="VF752_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8855912.1"
                     /translation="MDARVATPHRQPALDKVPAPGLVLGAVFSIQFGAALAATLFDDI
                     GPGGTVTLRLLFAAIVLVAVWRPRLASHTAADLRLAIVFGLVLGAMNAFFYEALDRIP
                     LGVAVTLEFVGPLGVAIVGSRRGLDLVWAGLAAVGIVLLGGGVGGANDALGVAFALIA
                     GGCWAAYILLNARLGKRFPGGSGLAIGMTASALAALPLGIADAGSDLLDPAVLAIGLA
                     VALLSSAIPYSLELEALRRVPPKTFGVLMSLEPAVATLSGLVVLGQSLHALDLLAIAL
                     VVAASIGATLEARRPEPPVAA"
     gene            7518..8156
                     /locus_tag="VF752_09950"
     CDS             7518..8156
                     /locus_tag="VF752_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX8855913.1"
                     /translation="MEVTVRLFAMLRERAGASELRLELPDGARVRDALEALGAVTDGL
                     PLVMAVNREYAPEDQELSPGDELALIPPVSGGEAALLHARVTSEPLSLDALATRVRDA
                     RAGAVVTFSGVTRQVERLEYEAYAEMAEERMAQIASEAVAAHGLCAAAVEHRVGDVPL
                     SEASVIVAVSAPHRGEAFAGAREIIDRVKAEAPIWKKEVEGGEGRWVEGRLP"
     gene            8119..>9086
                     /gene="selA"
                     /locus_tag="VF752_09955"
     CDS             8119..>9086
                     /gene="selA"
                     /locus_tag="VF752_09955"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX8855914.1"
                     /translation="MARDAGSRAGSPELSAALRALPSVEELAASLPDVPHTRAVAAAR
                     AVIGARRERILAGESPGGGLEAEARAWLERSGRPLLRRVINASGVIVHTNLGRAPLAP
                     SAAEAAATVGAGYSNLEYDLERGARGSRQAHVEQLVCELTGAEAALVVNNCAAAVLLA
                     AAALAGDCELIVSRGQLVEIGGSFRVPDVVAQSGARLVEVGTTNRTRISDYERAIGPD
                     TGAIMRAHQSNFRTLGFVEEPSIEQLCELGVRADVAVIDDVGSGALAEGMPELADEPA
                     VRRSVDAGAALVCFSGDKLLGGPQAGVMAGRADAVARCRTHPLARAL"
BASE COUNT         1430 a         3130 c         3219 g         1307 t
ORIGIN      
        1 gaagaagaag gcgaggcgcg gggcgaactc gtcgactccg aggccggcgt cgagcgccgc
       61 ctgcacgtac gcgatgccgt tcgcgagcgt gaacccgacc tcctggaccg ccgagcagcc
      121 cttctcgcgc atgtggtagc cggagatcga gatcgtgttc cacttcggga tccgctcctt
      181 ggtgtaggcg aacagatccg ttgtgaggcg catcgccggc acaggcgggt agatgaagtt
      241 gccgcgcgcg atgtactcct tgaggatgtc gttctgcgtg gtgccgcgca gctcctcggc
      301 cggcacgccc tgctcctcac cgacgagctc gtagaggagc agcaggaccg cggccggcgc
      361 gttgatcgtc atcgacgtgg acacctcggc gaggggaatg ccgtcgaaca cccggcgcat
      421 gtcgtcgatc gtgtcgatgg ccacgccggt gcggccgacc tcgccgaggc agagcggatc
      481 gtcggagtcg cggccgagct gggtgggcag gtcgaaggcc atcgagaggc cggtcgagcc
      541 gtgctcgatc aggtagtgga aacgctcgtt cgtctcctgc gccgtggcgt accccgcgta
      601 ctggcgcatc gtccacttgc gcgagcggta catgtcgggg tggatcccgc gcacgaaggg
      661 gtattcgccg ggctcgccga ggcgctcctc gaggccgggc gcgacgtcgc cctcctcgta
      721 gacgcgctcg atctcgatgc cggaatcggt gaagtggcgg gggtcgtcgg ggccgacgat
      781 cggggcgata gtgggctttt caggcttggt cgccatgggc ccgaaggata cgtgcggcgc
      841 tcaggcgagg gtgcgcccgc agagcgcggc atgggccgcc acgcgctcgg cgaactcggc
      901 gaagtcgcgc gcgtacagct gctgcgggcc gtcgcagagc gcggcctcgg ggtcctcggc
      961 cacctccacg atgatgccgt ccgcgccgat cgcggccgcc gcgcgagaga gcggctccac
     1021 cagcttgcgg tcgccagcgg catgcgaggg atccacgatc accggcaagt gggtgtgcag
     1081 cttcaggtat ggcactgcgc cgaggtcgag cgtgaagcgc gtagccgtct cgaacgtccg
     1141 gatgccgcgc tcgcacagga tcacgcggtc gttgccctcc ttgaggatgt agtcggccgc
     1201 catcaacagc tcctcgacgg tggaggagag gccgcgcttc agcagcaccg gcttgccgag
     1261 cttgcccacc acctcgagca gcgcgtagtt ctgcatgttg cgggccccga cctggatgac
     1321 gtcgaccacc tccgccaccg gctcggccca gttgatgtcg gtcagctcgc tcaccaccgg
     1381 cagcccggtg cgctcgcgcg cttcggcgag gatgtcgagc gccggtggcc cgagtccctg
     1441 gaacgagaac ggcgaggtcc gcggcttgaa ggcgccgccg cgcatcatcg acgcgccgcc
     1501 tgcctgcatc gcgtcggcga ccgcgagcgt ctgctcacgc gattcgaccg tgcacggacc
     1561 ggcgatcagg cagaacgtcg cattaccgcc cacgacgcga ccatcgaccg agaacgaggt
     1621 gggctcgtgg tggctgagct cgctcgacgc cagcttgtac ggccggctga tcgggatcac
     1681 ctgatccacg ccaggcatgc cctcgagcgc gagcgcccgc acgccctccg gatcgccgat
     1741 cgcgccgatc gctgtggtca gctcgccagg catgaccttc gcgtgcacgc ccacggcttc
     1801 gagccgctct accacggcgg cgatctcggc ctgcgaggcc tgaggtgtca tcacgaccat
     1861 catgcgatcg ccgtgaatca tggaccaccg ttgttagcat tgcctctccc gctggaccgg
     1921 gctcaagccg tgcttcatac cccgacattc gctcgtggag agcgcggccg cccctctggg
     1981 gccgcgacgc acccaggagg cgtcttttga tggcgaccga cggctcagcg gcggcggagc
     2041 tcacgtttcg cgacgtgacc aagcgctacg ccgggcgcgc ccagccagca gttgaccacc
     2101 tctcgctccg cattccggcg ggagagatct gcgtgctcgt gggaccctcg ggcgccggca
     2161 agaccaccgc gctgaagatg gtgaaccggc tgatccagtt cgactccggc gacatcgaga
     2221 tcgacggtcg cagcgtcctc gacttcgaga tctcagaact gcggcggggg atcggctacg
     2281 tgatccagca ggtcggcctg ttcccgcaca tgaacgtgga ggcgaacgtg gcgaccgtgc
     2341 cgcgcctgcg cggctggggc aagcagcgca cgcacgagcg cgtgcgcgag ctgctcgagc
     2401 tcgtgggcct cgacccggac ggggacccgc gccgctatcc cggccagctg tccggcggcc
     2461 agcgccagcg ggtaggcctc gcgcgtgcga tggcagctga cccgccgctg atgctgatgg
     2521 acgagccgtt cggcgcgatc gacccgatca cgcgctcgcg cctccaagac gagttcctgc
     2581 ggctccaccg ccagatccgc aagaccgtga tcttcgtgac ccacgacatc gacgaggcga
     2641 tcaagatggg cgaccgcatc gcgatcctgc gccagggcgg cacgctcgcc cagtacgaca
     2701 ccccggacgc gatactcgcg agtcccgccg acgacttcgt cgcgcagttc gtgggcgccg
     2761 accgcgcgct caagcggctc agccttgtgc gcctgtccga cctcgagctc gaggccgtgc
     2821 cgagtcaggt gaacggcctt ccgagcgcgc cgcgcgacgc gtcgctgcac gacgctcttt
     2881 cgctgttgct gagcgagggc gggagccgcc tgctcgtgac ggatcccgac ggcaccccac
     2941 tcggcgtcgc gcggctcgag cagatcggcg cgcgcgtgtc ggaggaccag gcctcatgac
     3001 gacgctgatc gcgcagtccg ggccggtgat cccgaacttc ggcggcggcg gcagcaacag
     3061 ctgcgtgcgc gagaaccgca ccttctgctg ggactggttc acctcgcact ggagctcgaa
     3121 cttccagccg cacctcgtgg agcacatcgt gctcacggcg atcgccgtga gcgtgggctt
     3181 cgtgatcgcg atggtggcgg cgctgttcgc ctaccgccgc aactggttcg agcagccgtt
     3241 cacgattgct accgggatcc tctacacgat cccgagcctc gcgctgttcc agatcctcgt
     3301 gccgatcact gggctcacgc gcctcaccgc cgagatcgcg ctcgtctcct acacgctcct
     3361 gatcatcttc cggaacatcc tcaccggcct gcgcgaggtg cctgaggacg tgcgcgaggc
     3421 cgcgcgcgga atgggcttga cgcgcaacca gatcctgtgg cgggtcgagc tcccgattgc
     3481 cgtgccggcg atcgtggcgg gcgtacgcat cgcgaccgtc accgtcatca gcctcgccac
     3541 ggttgcagcg atcgtgggcg tcaaccaggg gctcggcgtg ccgatcctcg acgccatccc
     3601 gacgacgttc aacacggaat tcatcgcggc ggcggtgctc acaatcgcgc tcgcgctcgt
     3661 ggctgatgcg ctgctcgtgg gcgcgcagcg ggtgctcacg ccgtgggcgc gagggagggc
     3721 ggcgtacaga tgaacgactt cgtcgacgcc ttcaagttca tgggcgacaa cctgtcgctg
     3781 ctgctcaacc gcacagtcga tcacctgctg ctgagtgggg cggcgatcgg cgtatcgctg
     3841 ctgatcgcgc tgccgctcgg attctggctc ggacacctgc accgcgcctc gttcctcgcg
     3901 atcaacgtgt cgaacatcgg ccgcgccctc ccgagcctgg cggtgatcgc catcggcatc
     3961 agcatcttcg gcctcgggtt tctgaactcg atggtggcgc tcgtggtgct agcggtgccg
     4021 atcatcctca cgaacgcata cgtggcggtt gacggggtgg acgacgacgt ggtggaggcc
     4081 gcgcgcggaa tgggcatgcg cccggtgcag gtgctcggcc gcgtggagct gtcgctcgcg
     4141 atcccgctca ccttcgccgg gatccgtacc gcggtcgtgt acgtgattgc caccgccacg
     4201 ctggccgcga tagctggcag cggcgggctc ggcgagatca tctacaacca ggcgagctac
     4261 ggcctcccgg gcgtgctcgc ggcctcgttc tgggttgccg ccctggcgct gctcgccgac
     4321 ctgctgctcg ccgcgcttca gcgcgctgtg atcccgcgcg ggctacggcc ggtgcgtcac
     4381 gccgatgcag ccgtcgcacc gcccgacgcg ctgcggtcga gcgaggtcgc ggcctgagat
     4441 ttgagggaga ggaagggttg agccaacgaa aggagcccca caggtgttga cgagagcaac
     4501 ccgacctgtc atcgcgcttg ctgtagcgct ggccatcgca ttggtggtcg ccgcttgcgg
     4561 cggcagcaac gacaacaacg gcagtggcaa cacgcagacg accggagcct cggcccagcc
     4621 cggaaaagga aagccaccgg tgacgatcgg ctcgaagaac ttcaccgagc agttcatcct
     4681 cggccagctg tacgcgcagg cgctgaaggc gaagggctac acggtgtcgc tcaagcagaa
     4741 cattggcagc tcggagatca tcgacaaggc gctcacgagc gacaagatcg acatgtatcc
     4801 cgagtacgtg ggcatcatcc tcactgccgt tgcgcacgag accgcgaagc ccaagagcgc
     4861 ggacgatgcc tatcagcagg ccaagaagtt cgaggagggt cgtggattca cggtgctcga
     4921 caagacgccg ttctccaacg tggacgcgat gggtgtcacg aaggcgttcg cacagaagaa
     4981 tgggctgacg acggtcacgg acctgaagaa gctcagctcg ttcaggctcg gtgggccgcc
     5041 tgagttcaag acacgcgaca ccggcgtcgt cggcctcaag aaggactacg ggctggacaa
     5101 catcaagttc gtgccgctcg cgatcgggct ccagtacaag gcactcgact cgggcaaggt
     5161 cgacgctgcg gacgtgttca ccaccgacgg ccaactccag ggtggcaagt acaccgtcct
     5221 gaaggacctg gagaacacgt tcgggttcca gaacgtggcg cccgtcgtga gtcagaaggt
     5281 cctttccaag gagggaacgg agttctcgaa gatcctcaac gacgtgagcg cgaagctcac
     5341 cgtcgaggcg atgcggaaaa tgaacgcggc agtggatctc gacaagcagg agccggcgtc
     5401 cgtcgcccag cagttcctga gcgcgaacgg cctcgtctag accgcgggag cggcgggggg
     5461 cttggcgcgc cgagcctccc gccgcgcacc gaggtgacgc cgctcggcgg cgagcaccgc
     5521 caccagcagg atcacgagcg cggcgagctg agcgaccgcg ctcagctccg tgccgagtgg
     5581 gagcgcggcg aacgcgagga gcgcggcgag cccgcgccag gcgccgctgc cgatccccag
     5641 cgttcgccgg aagagggcgt ccgcgcccat gaacagagcg actccgccgc tgagcgcgag
     5701 ggcgcgcgcg aactcgagcg gatcgccggc gtggccggtc gccttcttga tcgccgaggc
     5761 gaccgcgacg atcccgaaca gaatcggcaa atgcaggtag aagtacgcgt cgatcgcgag
     5821 ccgcggccgc gcctcgacgg gcgccgcgga cagtgcctct tctgcctcaa cgtcgtcgcc
     5881 agcgccgaag taggtccacc acagacacgc gctcagcgcg agcccgagga gcgccacgcc
     5941 cacgagctca gcgtcgacgg gcaggccggc cgctccgatc ccgatcgcaa cgacggactc
     6001 gccgatcgcg acgatcacca ccaagccgtg gcgctcgacg aagtgcgcgg gcgctatcac
     6061 gaagccctcg tcgctcgtga gccgcgggtt cgcgcactcg agcgcgactg ccactgccca
     6121 cagcacgtac tgcgtcgtgc cgccgatcgc tcccccgatc agcacgagcg ccgccgcggt
     6181 gatgttccag ggcaggatgc cgctgaacgc ctgggccact cccgcgggcg acgcgcgcat
     6241 gaacatgccg ccgtgaacca cgatcacgac gagatagccg agcgcgaacg tgagcccgct
     6301 gccgccgaag gcggtcggga gcgcgagcgc catcaccagg aagcccgcca tgccgccgag
     6361 cagcagcagg cggcgcgtgt cgcgatcgac cgtcaccgag ttcgtgagcc aggcgtagcc
     6421 gccgtacatc caccagatca gcgcgagcat cagcagcacc cgtgccacgc cgcggccgtt
     6481 cgggtccttc accagcacgg tggtgagctg ggtgatcgtg aacacgaaca ccaggtcgaa
     6541 gaagagctcg agcgtggtga cgcgcggggc ctgctcggtg ggctgggtca tgccgccacc
     6601 ggaggttccg gccggcgcgc ctcgagcgtg gcgccgatgc ttgcggcgac gacgagcgcg
     6661 attgcgagca ggtcgagcgc gtgcagcgac tggccgagca ccacgaggcc ggacagcgtc
     6721 gcgaccgccg gctcgaggct catcagcacc ccgaacgtct tcggcggcac ccggcgcagc
     6781 gcctccagct cgagcgagta cgggatcgcc gagctgagca gcgcaaccgc gaggccgatg
     6841 gccagcaccg ccgggtcgag caggtcgctg cccgcatcgg cgatcccgag cggcaacgcg
     6901 gcgagcgcgg acgcggtcat gccgatcgcg aggccgctgc cgccgggaaa tcgcttaccg
     6961 agccgcgcgt tcagcaggat gtatgccgcc cagcagccgc ccgcgatgag tgcgaacgcc
     7021 acgccgaggg cgtcattcgc cccgcccact ccgccgccaa ggagaacgat gccgaccgcg
     7081 gccaggccag cccagacgag gtcgagcccg cgacgcgaac caacgatcgc gacaccgagc
     7141 gggcccacga actcgagcgt gaccgcgacg cccagcggaa tgcggtcgag tgcctcgtag
     7201 aagaacgcgt tcatggcgcc gagcaccagg ccgaagacga ttgccaggcg gaggtcggcc
     7261 gcggtgtgtg acgccagccg cggtcgccac accgccacca gcactatcgc cgcgaagagc
     7321 aggcgcagtg tgacggtgcc gccgggcccg atgtcgtcga acagggtcgc cgcgagcgcc
     7381 gcgccgaact ggatcgagaa caccgcgccg agcaccagcc cgggagcagg caccttgtcg
     7441 agcgccggct ggcggtgcgg ggtggcgacg cgtgcatcca cgccgctcca cgctacccgg
     7501 cgcctagcat ctgttgcatg gaggtcacgg ttcgcctgtt cgcgatgctc cgcgagcgcg
     7561 cgggggcgag cgagctgagg ctcgagctgc cggacggcgc gcgcgtgcgc gacgcgctgg
     7621 aggcgctcgg ggcggtcacc gacgggctgc ctctcgttat ggccgtgaac cgcgagtacg
     7681 cgcccgagga ccaggagctc agccccggtg acgagcttgc gctgatccct ccggtgagcg
     7741 gcggcgaggc cgcgctgctg cacgcgcgcg tgacgagcga gccgctctcg ctcgacgcgc
     7801 tggcaacgcg cgtgagggac gcgcgcgccg gtgcggtcgt caccttctcg ggcgtgacgc
     7861 ggcaggtcga gcggctcgag tacgaggctt acgcggagat ggccgaggag cgcatggccc
     7921 agatcgcgtc cgaggcggtg gccgcgcacg gtctgtgcgc cgcggccgtc gagcaccgcg
     7981 tgggcgacgt gccgctgtcg gaggcgagcg tgatcgtggc cgtctcggcg ccgcaccgtg
     8041 gtgaggcgtt cgcgggcgcg cgcgagatca tcgaccgcgt gaaggccgag gcgccgatct
     8101 ggaagaagga ggtcgagggt ggcgagggac gctgggtcga gggccggctc ccctgagctg
     8161 agcgcggcgt tgcgggcgct gccctcggtg gaggagctcg ccgcctcgct gccggacgtg
     8221 ccgcacacgc gcgcggtcgc cgctgcgcgc gcggtgatcg gcgctcggcg tgagcgcatc
     8281 ctcgccggcg agtcgcccgg cggtggcctc gaggcggagg ctcgtgcctg gctcgagcgc
     8341 tccgggcgcc ccctccttcg gcgcgtgatc aacgcgagcg gagtgatcgt gcatacgaac
     8401 ctcgggcgcg cgccgctcgc gccctccgcc gcggaggccg cagccacggt cggcgccggc
     8461 tactcgaacc tcgagtacga cctcgagcga ggcgcccgcg gctcgcggca ggcgcacgtg
     8521 gagcagctcg tgtgcgagct caccggcgcc gaggcggcgc ttgtagtgaa caactgcgcc
     8581 gcagcggtgc tcctggcggc ggccgcgctc gccggcgact gcgagctgat cgtgtcgcgc
     8641 gggcagctcg tggagatcgg cggctcgttc cgcgtgccgg acgtggtggc gcagtcaggc
     8701 gcgcggctcg ttgaggtggg cacgacgaac cgcacgcgga tctcggacta cgagcgcgcg
     8761 atcggacccg acacgggcgc gatcatgcgc gcgcaccagt cgaacttccg cacgctcggc
     8821 ttcgtcgagg agccgtcgat cgagcagctc tgcgagctcg gcgtccgagc cgacgtggca
     8881 gtgatcgacg acgtgggttc gggcgcgctc gccgagggca tgccggagct cgccgacgag
     8941 cccgccgtgc gccgctcggt ggacgccggg gccgcgctcg tgtgcttctc cggcgacaag
     9001 ctgctcggtg ggccgcaggc gggcgtgatg gccggccgcg ctgacgcggt cgcgcgctgc
     9061 cgcactcatc cgctcgcgcg cgcact
//
LOCUS       DASVXS010000178         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_330213, whole genome shotgun sequence.
ACCESSION   DASVXS010000178 DASVXS010000000
VERSION     DASVXS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330213"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VF752_09960"
     CDS             complement(<1..702)
                     /locus_tag="VF752_09960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX8855915.1"
                     /translation="MELTAALTRYFGFPGFRPGQHEACEAALASRDVLVVMPTGSGKS
                     LCYQLPALLREDLTVVVSPLVALMQDQVEALHARGLGHSVALLNAQQDQAANADALAR
                     AVGGKLRLLYVAPERFSSPGFVARMREARVGLFVVDEAHCVSQWGHDFRPDYFRLADA
                     ARYLGADSIVASTATATPGVAADVVRRLGLREPLRVATGFDRPNLSFAVARPAPHEKH
                     VLLVETLRAPDALPAI"
     gene            complement(789..1967)
                     /gene="purF"
                     /locus_tag="VF752_09965"
     CDS             complement(789..1967)
                     /gene="purF"
                     /locus_tag="VF752_09965"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX8855916.1"
                     /translation="MDLAHQLGADDVEGAALGGDDEALAHNGNLTNAVELWGELKQRG
                     IDFRGTSDSEIIAALLSSHEGKMIEDAVADVMPRLQGAYSTVVMTKRAIVAFRDPHGI
                     RPLSLGRIGDRFVVASESCAFDIIGAELMREVHPGEMVSLTERGLETRQVVKTDRRAS
                     CVFEHIYFSRPDSRLEGKVLQQVRGRMGEILWREAPVEADLVISVPDSGNPAANGYST
                     ASGIPKDDGLIKNRYVQRTFIQPGQELRKHGLRMKFNPLPEIVAGKRIVVVDDSIVRG
                     NTTRQIVKMLRDAGASEVHMRISAPPIRHPCHYGIDMSTTQEMIAHGRTVEEVSEELG
                     CDSLAYLSLDGLYEAIDASRATHCDACFSGDYPLERTHAARGKYALEEPDLEVRLPVA
                     "
     gene            1953..>2171
                     /locus_tag="VF752_09970"
     CDS             1953..>2171
                     /locus_tag="VF752_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855917.1"
                     /translation="MREVHPGEMVSLTERGLETRQVVKAERRATCVFEHIYFARPDTI
                     LEGNRTQVSRRKMGEILWREAPVEADVVI"
BASE COUNT          335 a          750 c          774 g          312 t
ORIGIN      
        1 gatcgccggc agagcatcgg gggcccgcag agtctccacg aggagcacgt gcttctcgtg
       61 cggcgccggg cgcgccacgg cgaacgacag attcggccgg tcgaacccgg tcgccacgcg
      121 gagcggctcg cgcagaccca agcgccgcac cacatcggcg gcgactcccg gcgtcgcggt
      181 ggcagtggac gcgacaatcg agtccgcgcc cagataccga gcagcgtcgg ccaggcggaa
      241 gtagtccggc ctgaagtcgt gcccccactg cgacacgcaa tgcgcctcgt ccaccacgaa
      301 caggccaacc cgcgcctcgc gcatgcgcgc cacgaagcca ggcgaggaaa agcgctcggg
      361 ggccacgtac agcaggcgga gcttgccgcc cacggcccgc gcgagcgcgt cggcgttcgc
      421 cgcctggtcc tgctgcgcat tcagcagcgc gacggagtgg ccgagcccgc gcgcgtgcag
      481 agcctcgacc tggtcctgca tgagcgcgac gagcggcgag accaccacgg tgaggtcctc
      541 gcgcagcaac gccggcagct ggtagcagag cgatttgccc gagcccgtgg gcatgacgac
      601 gagcacgtcg cggctcgcga gcgcggcctc gcatgcctcg tgctgccccg gccggaagcc
      661 cgggaagccg aagtaccggg tgagtgcggc ggtgagttcc aaggcaggcg agggtacctg
      721 tgcggtcggc gggatcaggc cccgcaacag gcgctcgtca cggtgccgta acgcaacgtg
      781 cggactattt acgcgaccgg cagccggacc tcgaggtccg gctcctcgag cgcatacttg
      841 ccgcgtgcgg cgtgggtgcg ctcgagcggg taatcgccgc tgaagcaggc gtcgcagtgc
      901 gtcgcgcgcg aggcgtcgat ggcctcgtac aggccgtcga gcgagagata cgccagcgag
      961 tcacagccga gctcctccga cacctcctcg accgtgcggc cgtgggcgat catctcctgc
     1021 gtcgtcgaca tgtcgatccc gtagtggcag ggatggcgga tcggcggcgc cgagatccgc
     1081 atgtgcacct ccgaggcgcc ggcgtcgcgc aacatcttca cgatctggcg cgtggtgttg
     1141 ccgcgcacga tcgagtcgtc caccacgacg atccgcttgc cggcgacgat ctctggcagc
     1201 gggttgaact tcatccgcag accgtgcttg cgcagctcct ggccgggctg gatgaaggtg
     1261 cgctgcacgt agcggttctt gatcaggccg tcgtccttcg ggatgccgga cgccgtcgag
     1321 tagccgttcg cggcggggtt gcccgagtcg ggcaccgaga tcacgaggtc ggcctcgacc
     1381 ggcgcctcgc gccagaggat ctcgcccatc cgcccgcgca cctgctgcag gaccttgccc
     1441 tcgagccggc tgtcggggcg ggagaagtag atgtgctcga aaacgcagct ggcgcggcgg
     1501 tcggtcttga cgacctgccg cgtctccagc ccgcgctcgg tcagcgagac catctcgccc
     1561 gggtggacct cgcgcatcag ctcggcgccg atgatgtcga aggcgcagct ctcggaggcg
     1621 acgacgaagc ggtcgccgat ccgcccgagg ctgagcggcc ggatcccgtg cgggtcgcgg
     1681 aaggcgacga tcgcccgctt ggtcatcacc acggtcgagt aggcgccctg cagccgcggc
     1741 atcacgtcgg ccacggcgtc ctcgatcatc ttcccctcgt gggaggagag cagggcggcg
     1801 atgatctccg agtcggaggt gccgcggaag tcgatccccc gctgcttcag ctcaccccac
     1861 agctcgaccg cgttggtcag gttgccgttg tgggcgagcg cttcgtcgtc gcctccgaga
     1921 gctgcgcctt cgacatcatc ggcgccgagc tgatgcgcga ggtccacccc ggcgagatgg
     1981 tctcgctgac cgagcggggg ctggagacgc gccaggtggt gaaggccgag cggcgcgcga
     2041 cctgcgtctt cgagcacatc tacttcgccc gcccggacac gatcctcgag ggcaaccgca
     2101 cgcaggtgtc gcggcgcaag atgggcgaga tcctctggcg cgaggccccg gtcgaggccg
     2161 acgtggtgat c
//
LOCUS       DASVXS010000179        20189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_659865, whole genome shotgun sequence.
ACCESSION   DASVXS010000179 DASVXS010000000
VERSION     DASVXS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20189
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659865"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="VF752_09975"
     CDS             <1..1862
                     /locus_tag="VF752_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855918.1"
                     /translation="VTGSSRGSYAADAKASRRALPFYFKPTGLGTYLLYDSTAGLVSI
                     ASTQQVVRADAPAPAAEWRTQRRARGLVALRSTSSGRLLAVDRASRALVTVSRNAPAA
                     SFRLTPARGCRSYPEAALGARGKPPAAKRADGSLIGFADPHLHVTAEFRAGGQVISGQ
                     TFDRFGITEALGHDADAHGNDGALDITGNLLRSGQPAGTHDTHGWPTFAGWPTFDTYT
                     HQQVYYRWLQRAWMGGLRLIVAQTVEDEPLCNIEPRKSHSCDETETIELEVRRLRALQ
                     NYVDAQSGGRGRGWFRLVYSPRQARQVILRGKLAVIIGVESSNPFGCSEFMGQPHCDR
                     AGIDRGIARMRRIGVRSMFLAHWVDNALAGAALEGGDKGTFIGTMQVAQTGRPFATGP
                     CPHPGQGEEVPATSVLGELLPVHPAARQCNTRGLTELGAYAVRQLMDNHMLIEVDHLS
                     EWARDQVLKIAEERRYPVVSSHTGTGGSWDPSELRRLYAVGGFASATIDDAAKLPDKI
                     LTFRRYTSAKEPAVGFATDTGGFAALPGPDPRGKQDPLRYPFRAYGSHVRFERQRTGS
                     RTFDINTDGVAHYGLVPDLLANVQREKNGRRALALLFHSADAYLRTWRLAAGK"
     gene            1914..2612
                     /locus_tag="VF752_09980"
     CDS             1914..2612
                     /locus_tag="VF752_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855919.1"
                     /translation="MSVNDLVLVQGMADTRASLRRWRRDPNAVLAPWLLLSLAIAVAL
                     LLAVWAIAMLSTPDPTPLFLPGVHFDPTPVNVVKVLYRNSLVLALHAMACVAGFIAGS
                     SLPQQAQQKTGLSKKIHDHAGRLAIGFVVCATTFSLVTQAYVLGGGAATLAYELGISR
                     GVLLLALLPHALPELVALFLPLAAWLIASRRGAWDELLAATFATVAVAAPTLIAAALV
                     EIYVTPQLLIALTG"
     gene            complement(2685..3422)
                     /locus_tag="VF752_09985"
     CDS             complement(2685..3422)
                     /locus_tag="VF752_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855920.1"
                     /translation="MRKKPLAGAVIGVLALATASVAIAGTKNGVTFDATTTVKKPGTS
                     TGLKTHIQGAPTFNGQLEAARQVTVSFPPGTKINSKAVAQCKATDQQIQSTNGAACPA
                     KSKIGTGIAEAITGLGPSIDPVVENIVAFNVPKGIAFLLTPKGAIGQTAAFRGKFSGA
                     GASSVHGAAAGPKLVTNVPKFPIPGVGEAKLTKFDLNVKKGGTRAKPYATTPRTCPKS
                     GKWALTALFKYDNTPPILVKDTSPCTK"
     gene            complement(3570..4310)
                     /locus_tag="VF752_09990"
     CDS             complement(3570..4310)
                     /locus_tag="VF752_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855921.1"
                     /translation="MRRKPLAGALACALAATAVGVAAAETVDGVTFTAKTTTTKPATS
                     TGLNVLIAGTPTGPDGKPIATREVTVVFPAGTKINTKAIPQCKATDQRIQSTNGSACP
                     AKSKIGTGTAEAFTDLGPAVDPVAEAIVAFNVPKGIAFLLTPKGPIGQTAAFRGKFSG
                     AAAANAGAAAAGPKLVTAVPKFPIPGGNEAKLTKFALKVKANGKGAKAYSLTPKTCPK
                     SKKWSLSAIFKYDTHAPITVTTSGPCKK"
     gene            complement(4383..5099)
                     /locus_tag="VF752_09995"
     CDS             complement(4383..5099)
                     /locus_tag="VF752_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855922.1"
                     /translation="MGMKPIAGAVAGVVVAAVAGVAIATPGTSDGVTWDAKTTTPKVN
                     TPTGLSTTIKGAPVGADGKQVPIRKVTVQFPPGTKINTKALPQCTASPQTIQNTNGNV
                     CSKSRVGTGLVKANTGLGEGVDPLSEDVQAFNGKKKIFFLLTPKGAVGQTAVLTGTFS
                     GTTAPKLVVDVPLFPIPGGSNAVLIQFDFAVKKTGTGKRAYGTTPKTCPKSGKWKLPA
                     TFGYEGHADIHVTATGPCRR"
     gene            complement(5213..5935)
                     /locus_tag="VF752_10000"
     CDS             complement(5213..5935)
                     /locus_tag="VF752_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855923.1"
                     /translation="MRSKKLLISAVVGVLALAVTAVAIASPQFKQTASIRYSSKKPRS
                     NTGVAVSLKAQDPGAPNGKPSPATKVTVTLAKGTKVDTSAAKVCTATDSQFASQGANA
                     CPAGSRLGTGSAEAITGLGAGIDPVGENIIAFAGKSQIIFLLTPKGAVGQTATIRGKV
                     RKNVVTVTVPAFPIPGAGNASLTVFNVALKSISSGRGSKKKNLVTTPSTCPASKNWTT
                     ATKFNYENGTTATIKSRSSCKR"
     gene            complement(6046..6690)
                     /locus_tag="VF752_10005"
     CDS             complement(6046..6690)
                     /locus_tag="VF752_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8855924.1"
                     /translation="MEFFERLEAERGRYDVLSHPFYTRWSAGELSREELALYAGQYRH
                     AVVALAAESRAAADAADDPAARTELELHAREEEAHVELWDRFVHSLGGATDAAASAET
                     ERCVAAWTAGDSQEERLAVLYAIESAQPAISQTKLDGLAAHYGVAADDDAASYFAVHS
                     RRDEEHAAQARRLIEANCRHCDDSRLLELALGALEGNWDLLDGVERAISAEIRL"
     gene            6747..7538
                     /locus_tag="VF752_10010"
     CDS             6747..7538
                     /locus_tag="VF752_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855925.1"
                     /translation="MRAPADLQIHAPEFPPRLPWLNVAFLRMDKQLGQHAVLVEFWDF
                     ARINSLRTLPYMKAWHERYADAGLRVVGVHTPGYSFGRDQDAVGLAVSRLGIDYPVAI
                     DSDYLVWQEYGTQGWPSRYLFDRRGILRYVHYGEGDYLDAELAIQEVLEELDQDAVFP
                     PPLEPLRPEDEPGALLTPQTADIALPADRDRVALVRDWIDGPDYIEAADAGASATASF
                     TAGGAWAVLSGDGVEPGLYETGGTIVAESPGLRLHGFQFTPLPPE"
     gene            complement(7672..8628)
                     /locus_tag="VF752_10015"
     CDS             complement(7672..8628)
                     /locus_tag="VF752_10015"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX8855926.1"
                     /translation="MAEAPQKLLLAAPRGYCAGVDRAVQTVERALELYGAPVYVRKEI
                     VHNKHVVDQLRQRGAIFVDEETEVPEGQTVVFSAHGVAPSVHENAAARRLQTIDATCP
                     LVTKVHVEARKFAAQDYTIVLIGHSGHEEVEGTMGEAPGNIVLVESVEDVDALEVPDP
                     EHIAYISQTTLSVDETREIIVRLRERFPNIVGPRTDDICYATTNRQMAVRQMASECDL
                     VLVIGSRNSSNSNRLVEVARDHGARSHLIDNVGQLEDEWLEGARVVGITSGASAPEEL
                     VQELVDWFRQRGTTDISEFEVVQEDVRFMLPKEIRKALAAAS"
     gene            complement(8633..9547)
                     /locus_tag="VF752_10020"
     CDS             complement(8633..9547)
                     /locus_tag="VF752_10020"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8855927.1"
                     /translation="MNRHNLRVRILFAVLAVQLVLGATVATLAATNSLPFPGKHGSPD
                     PVPARLKADRFDAGRAWRLLKLQLSFGPRPAGSESSRRLAETLRRKLPHGRFQAVPGG
                     LRNVIGSVPGKDPHRIVVVGAHYDTKDIPGFLGANDGASGTAVVLELAGRLKPREARP
                     TVVFALFDGEESPAGTPDSQFEQYGLRGSRVAAARYPHAAAMILFDFVGDRELRLPRE
                     RSSNTRLWGKLRSAARRVGAIGAFPLASEARITDDHTPFLSEGVPSIDLIDWDFACFH
                     RTCDNLSAVSPKSLDAVGESVAQLLRTL"
     gene            complement(9544..9786)
                     /locus_tag="VF752_10025"
     CDS             complement(9544..9786)
                     /locus_tag="VF752_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8855928.1"
                     /translation="MADGEEGAAVASAMIALGATLGLETVAEGIEHAEQLERLRDLRC
                     DKGQGFYLARPLERDGIDALLAERESDWAGLSDKRP"
     gene            9862..11325
                     /locus_tag="VF752_10030"
     CDS             9862..11325
                     /locus_tag="VF752_10030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8855929.1"
                     /translation="MPTAHMDRLTALDASFLAQERKSSHMHVGAVLLFEGPPPHRAEF
                     TEQVESRLHLVPRYRQKLAFPPLELGRPLWIDDPRFNIEYHVRHTALPSPGSLDQLRQ
                     LAGRIFSQRLDREKPLWEIWLAQGLTENRWALITKTHHAMIDGVAGVDLASVLFDLDP
                     VPQPPSEPAEQWAPHPEPSSADLAAEGIKGLVKRPFSLAGRAIGAATKPAETAREVLE
                     AAEAVGEVAWAGLNPAPETPLNVDIGPHRRVWWVESQLSDFKEVKNTLGGTVNDVVLA
                     VVTGALRRWLRQRGVRTEGLELRALVPVSIRSEAERGDLGNRIAAMRGPLPVYCEDAV
                     QCLEIVSEAMANLKESKQALGAEVIAGLQDFAPPTLLAQASRLNFSTRLFNMIVTNVP
                     GPQFPLYMLGRELQDLIPIAFLPEGHALAIAIMSYNGKITFGLLADYDAMPDLDDFAM
                     YLEDSLGELLDASARAKRRKQDAAEPKPDAAKAPSSS"
     gene            11399..12931
                     /locus_tag="VF752_10035"
     CDS             11399..12931
                     /locus_tag="VF752_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855930.1"
                     /translation="MNRSVLALLLAMAAAALLAPVAYASDVQHLHFRYGPINVKPGSN
                     QILATGGVEKPSQDGYITRIVPNLKRADGSIPGTQLLHLHHGVWLNASRRDPTTPAFP
                     FEKFFAAGEEKTSMNLPAPYGYPVKASDVWILNYMIHDLVTRPFKVYITYDVDFVPAH
                     SALGKKMKPVRPIWMDVQNDHAYPVFDVHKGSGRNGRFTYPDQDPGAYPAGTPPLNEW
                     TADRSTTLVAMTMGHVHPGGLWDDLDVVRAGAQPNTAKGVIPGHVPHSVRIFRSKAKY
                     YDPNGPISWDVSLPVSKPSWRVHLNKGDKLRISTTYDSKHASWYEAMGIMLAYQTEGD
                     DGGPDPFRTAVDTKGRLTHGHLKENDVYGGRMPSLPNAKKLPGRFVKGNEVQIQGYKF
                     LPGDLRSRLGKPYVPEVRQGQSLRFVNGDGPPGLGFLKQVSHSITSCAFPCNLSTGIS
                     YPIAGGSGFDSGQMGFGRPGFTAFNNRQTWQSPTNLKPGTYTFFCRIHPFMRGAFRVV
                     GQ"
     gene            13062..13961
                     /locus_tag="VF752_10040"
     CDS             13062..13961
                     /locus_tag="VF752_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855931.1"
                     /translation="MGKVRVTHITDPGCPWAYSAEPAVNALRFRYGNQLEWRTVMIGL
                     TESGKEYEVRGYTPELMAATPLRFERYGMPFTAGLARTRVPGTGRACRAVVAARLIDP
                     DLGEIALRALRFAWFNTDLLLDEDANIAEALSRAPQLDARAIMSRIESPDVEAAYQQD
                     RALARSAEGTAAHMQGKTARTDGPERYTAPSLIFEADGQRLIAGGWQPLSAYDVLIAN
                     LDPTLSRRPPASDPLEALAEFPYALTTREIATLMAERDDEPDDAAAGEELVRLAARRQ
                     VERVPLGNDGLWRRVAAVAAAAA"
     gene            complement(13958..14698)
                     /locus_tag="VF752_10045"
     CDS             complement(13958..14698)
                     /locus_tag="VF752_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8855932.1"
                     /translation="MAQIVLVHGALLDGSSWGEVIFTLQRSRHEVVAAQLPLTGVEQD
                     AAVVRCEIDQMPSPCIVVGHGYGGAVVCEAARGAENVKALVFVAAFVPDEGESVADLM
                     ARYPQPAGWEPYIVPGDDGTFIVDRAHFARVVAPDVDPVTAQIMATAQGPTANTAFST
                     DTVGHPAWKDVRSYYLIADSDQVVHPATQRWMAERINAVTVIVAGTSHVPMQSQPDEV
                     ATLIERAANPPITGWPLRPPNEDPAPGT"
     gene            14795..15115
                     /locus_tag="VF752_10050"
     CDS             14795..15115
                     /locus_tag="VF752_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX8855933.1"
                     /translation="MPGAVEEFPFGPRTSVFKVGGKMFALSALDARPLSVSLKCEPEL
                     AEQLRLSYAAITPGYHLNKRHWNTVELDGSVPDKMVRDMVEDSYDLVVDGLPASKRRT
                     ISAT"
     gene            complement(15055..15852)
                     /locus_tag="VF752_10055"
     CDS             complement(15055..15852)
                     /locus_tag="VF752_10055"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8855934.1"
                     /translation="MATARLHCLFRGVYAVGHQALPKRGWERAAVLACGPDARLSHRH
                     AGDLWEVRATSRRVIDVTTPRGRAGQRGIQVHRVRALDPRDTDEVDGIPVTSLARTYL
                     NLAEVVKPRQVQRAIEEGDRLGIFDLREIEDVCERSPGRRGVKVLRALLTDAEEPPLT
                     RSELERLFLGVCHEYGIPRPAVNAIVEGVEVDMLWEKERVIVELDGYAYHRGRGAFER
                     GRERDAVLTLVGYRVIHITYRRLTREPDQVAEIVLRLLAGSPSTTRS"
     gene            complement(16085..17038)
                     /locus_tag="VF752_10060"
     CDS             complement(16085..17038)
                     /locus_tag="VF752_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX8855935.1"
                     /translation="MAVVLAIIAVVVFLAVIILASSIRVLREYERGVIFRLGRLIAQK
                     GPGLIFLIPIIDRMVRVDLRTVTLNIPPQEVITRDNVTARVNAVAYFRVIDPNKAIVE
                     VENFLVATSQIAQTTLRSVLGTADLDSLLSERERLNQSLQAIIDDQTEPWGVKVTTVE
                     IKDVEIPAQMQRAMARQAEAERERRAKIINAEGEYQAAQRLYEAAEIISRNPATLQLR
                     YLQTLLELGSGQSSTIVFPLPVDLLGPFMKILGSGGGDGDGGGPPARPPGPPPEPLVE
                     PGLAPHTDEPGTRVQPGLAPRGSPSDAEPDEEPDAGEHPPP"
     gene            complement(17023..18399)
                     /locus_tag="VF752_10065"
     CDS             complement(17023..18399)
                     /locus_tag="VF752_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX8855936.1"
                     /translation="MRVPALLIVLGLLMWVAAGSAVGQSGGATPSTGKAPIAYSIELN
                     ATIDPATQKWIDKALKEAADKRATIAIIRMDTPGGLDSSMRDMIKHIIAAPMPVVVYV
                     YPEGSRAASAGLYITESADVAAMAPQTNIGSATPINGNGQDIGKTLGRKIRNDAAAYV
                     RALAGAHGRNADLAEKMVRSAVNVPASEALDQHLIDIVATNQQDLLRQLDGFKVKGPK
                     AQTLHTANLSIVKRDMPFQYDLLELLVNPTIAYLLLTAGFAGIAFELFNPGIVAPGAL
                     GAVALVLGLFGTSQLPVNAAGVVLLLVALVLFVLELKITSHGVLGIAGVIALAAGGLL
                     LFNTDNSAFEISVPVVIFTAAALGGLTLFAVSKALEARRRRVHTGWEELVGRIGEVRV
                     PINPAGQVFVQGALWRARASDDSASFAIGEKVRVDSVDGLTLFVSAAPADLEGKEIEG
                     GEPAWQSS"
     gene            complement(18439..19518)
                     /locus_tag="VF752_10070"
     CDS             complement(18439..19518)
                     /locus_tag="VF752_10070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8855937.1"
                     /translation="MKIANVRIPKEEERLLALRVAGVLWLVAAVTSLAGAALLPHVDA
                     NMPVVGILCVLEVAWGAFCVLFPWNRVTAGWRYGAAVFHLPTILSFAFVVAIVWATGG
                     TDSFANYYLMFIVAYVASFYPPRLVAVYIGIALLVVAAPLLYQEDAVADGAYQLIWGA
                     APAYVVLGSVIAVGKAHITRLRDAASTLSLTDHLTGLANRRAFEERMSVRIGGGRASD
                     ATGLLLVDLDDFKETNTLHGHPGGDLVLREAATALREAARADDLVARLGGDEFAIVAN
                     GADEGGMRALAERVLDAVRRAHERLPGEFGGLSITASVGWARYPHDADDLDELLAAAD
                     FCLRGVKANGKNGSLTPLDWMPERV"
     gene            complement(19569..19874)
                     /locus_tag="VF752_10075"
     CDS             complement(19569..19874)
                     /locus_tag="VF752_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX8855938.1"
                     /translation="MAEVTVEDVQEAITNVIDPELGLDFIELGLVYDISVDQDGDVDI
                     TFTLTSPGCPIGPQVSEQMQEYVGELDGVREVRPRMVFSPPWTPDLMSEEAKFALGF"
     gene            19901..>20189
                     /locus_tag="VF752_10080"
     CDS             19901..>20189
                     /locus_tag="VF752_10080"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX8855939.1"
                     /translation="MPLFKLLVVFIVVPLAELYVIYKIGDAIGILPTIALLALDSVAG
                     SLLLKSQGRAVWTKFRQALEQGRIPQREVLDGVLVIFGGAFLITPGFLTDII"
BASE COUNT         3090 a         6992 c         6798 g         3309 t
ORIGIN      
        1 acgtgaccgg atcttctcga ggttcgtacg cagccgacgc caaggccagt cgccgcgcgc
       61 tgccgttcta cttcaagccc accgggctcg gcacctacct cctctacgac tcgaccgcgg
      121 ggctggtgag catcgcgagc acccagcaag tcgttcgagc ggacgcgccc gcgcctgcgg
      181 ctgaatggcg gacgcagcgg cgcgctcgcg gcctagtcgc tcttcgctca acctcgagcg
      241 ggcgcctcct ggcagtggac cgagcgtcgc gcgcgctggt gaccgtgtcg cgcaacgcac
      301 ccgccgcgtc attccgcctc acgcccgcgc gcggatgccg ctcgtacccc gaggccgcgc
      361 tcggggcgcg cggcaaaccg ccggccgcaa aacgcgccga tggcagcttg atcggtttcg
      421 ccgaccccca tctgcacgtg actgctgagt tccgagccgg cggacaggtg atctccggcc
      481 agaccttcga tcgcttcggc atcacggagg cgctcggtca cgacgccgac gcccacggga
      541 acgacggcgc cctcgacatc accggcaacc tgctccggtc ggggcaaccg gcggggacgc
      601 acgacacaca cggctggccg acgttcgccg gctggccgac gttcgacacg tacacgcatc
      661 agcaggtgta ctaccggtgg ctccagcgcg cttggatggg cggcctgagg ctgatcgtgg
      721 cacagaccgt ggaggacgag ccgctctgca acatcgagcc gcggaagagt cactcctgcg
      781 acgagaccga aacgatcgag ctcgaggtcc ggcggctccg cgcgctgcag aactacgtcg
      841 acgcgcagag cggcggccgc ggccgcggtt ggttccgcct ggtgtacagc ccgaggcagg
      901 ctcgtcaggt gattctgcgc ggcaagctcg ccgtgatcat cggtgtcgag tcttcgaacc
      961 cgttcggctg cagcgagttc atgggacagc cgcactgcga tcgcgccggc atcgaccggg
     1021 gcatcgcgcg gatgcgccgg atcggagtgc gcagcatgtt cctggcgcac tgggtcgaca
     1081 acgcgctggc gggtgccgcg ctcgagggtg gcgacaaggg cacgttcatc ggcaccatgc
     1141 aggtcgctca gacgggccgt ccgttcgcga ccgggccgtg cccgcacccg ggtcaaggcg
     1201 aggaggtgcc ggccaccagc gtgctcggcg agcttctccc ggtgcacccg gccgcacgcc
     1261 aatgcaacac gcggggcctc acagagctcg gcgcctatgc cgtgcgacag ctgatggaca
     1321 accacatgct catcgaggtc gaccatctca gcgagtgggc tcgcgaccag gtgctgaaga
     1381 tcgccgagga gcgacggtac ccggtcgtct cgagccacac gggcaccgga ggatcatggg
     1441 acccgtcgga gctgcgacgg ctctatgccg ttggcggctt cgcctcggcg acgatcgacg
     1501 acgccgccaa gctgccggac aagatcctca ccttccgccg ctacacctca gcgaaagagc
     1561 cggctgtcgg attcgcaacg gacaccggcg gcttcgcggc gctccccggt ccggacccgc
     1621 gcggcaagca ggacccgctg cgctacccct tccgcgcgta cggcagccac gtgcgcttcg
     1681 agcggcagcg caccggatcg cgcacgttcg acatcaacac cgacggtgtg gctcactacg
     1741 ggctggtgcc cgatctactc gccaacgtgc agcgggagaa aaacggccgg cgcgcgctgg
     1801 cgctgctgtt ccattccgcg gacgcctact tgcgcacctg gcgattggcc gccgggaaat
     1861 agcttcacct ccccagcctc aacccgaggc gccccgttgc cgatggttga gccgtgagcg
     1921 tcaacgacct ggtcctcgtg caggggatgg ccgacacgcg cgcttcgctg cggcgctggc
     1981 ggcgcgatcc gaacgccgtg ctcgcaccgt ggctgctact gagcctggcg atagcggtcg
     2041 cactgctcct tgccgtgtgg gcgatcgcga tgctgtccac gcccgacccg acgccgctgt
     2101 tcctgcccgg ggtccacttc gaccccacgc cggtcaacgt cgtcaaggtg ctgtaccgca
     2161 actcgctcgt gctcgccctt catgcgatgg cgtgcgttgc aggcttcatc gcgggcagct
     2221 cgctgccgca acaggcccag cagaagaccg gcctctccaa gaagattcac gaccacgccg
     2281 gccggctcgc aatcggcttc gtggtctgtg ccaccacgtt ttcgctcgtc acacaggcgt
     2341 acgtgctcgg cggcggcgcg gccacgcttg cgtacgagct cggcatctcg cgcggagtcc
     2401 tgctgctcgc gcttctcccc cacgcgctcc ccgagctggt cgcgctgttc ctaccgctcg
     2461 cggcctggct gatcgcaagc cgccgcggcg cctgggacga gctcctcgcg gcgaccttcg
     2521 ccaccgtggc ggtcgccgcg cccacgctga tcgccgccgc gctggttgag atctacgtca
     2581 cgccgcagct gctgatcgcg ctcaccggct gaaaagccgg cggccccgcc gaagcggggc
     2641 cgccgaggtc cgacgccggg ctttgccggc tgtcggccag cgttctactt ggtgcagggc
     2701 gaggtgtcct tcaccaagat cggtggcgtg ttgtcgtact tgaagagcgc cgtgagggcc
     2761 cacttgccgc tcttcgggca ggtccgcgga gtggtcgcgt agggcttggc cctcgtgccg
     2821 cccttcttga cgttgaggtc gaacttcgtg agcttcgcct cgccgacgcc cgggatcggg
     2881 aacttcggca cgttggtcac gagcttcggc cccgccgccg cgccatgcac gctcgacgca
     2941 ccggcaccac tgaacttgcc gcggaaggca gccgtctgtc cgattgcacc cttcggcgtg
     3001 agcaggaacg cgattccctt cggcacgttg aaggcgacga tgttctcgac gaccggatca
     3061 atgctcgggc cgaggccggt gatggcctca gcgatgccgg ttccgatctt gctcttcgcc
     3121 gggcacgccg cgccgtttgt gctctgaatc tgctgatcgg tcgccttgca ctgggcaacg
     3181 gccttcgagt tgatcttggt gccaggcggg aagctgaccg tgacctggcg agccgcttcc
     3241 agctggccgt tgaacgttgg cgcaccttgg atgtgcgtct tgagaccggt cgaagtgccg
     3301 ggcttcttca ctgtcgtcgt cgcatcgaac gtcacgccgt tcttggtgcc ggcgatcgcc
     3361 acgctcgcag tagccagggc gagcacgccg atcactgcac cggcaagcgg cttcttccgc
     3421 atttctcctc ctcttccgga taggccaggc gggcgcaacc ctacacgggc ctcacatgga
     3481 ttgcatcccc tgccacaaac tctttagagg ttgccgaaaa ggtgaaggga gtggtcgcac
     3541 gggcgaccgc tccctcccga aacagcgacc tacttcttgc acggaccgct cgtggtgacc
     3601 gtgatcggtg cgtgcgtgtc gtacttgaag atcgccgaga gcgaccactt cttgctcttc
     3661 gggcaggtct tcggcgtgag cgagtaggcc ttcgcgccct tgccgtttgc cttcaccttg
     3721 agagcgaact tcgtgagctt cgcctcgttg ccgcccggga ttgggaactt cggaacggcc
     3781 gtgacgagct tcggtccagc agccgcagct ccggcgttcg cggccgctgc tcccgagaac
     3841 ttgccgcgga atgccgcagt ctggccaatc gggcccttcg gagtgagtag aaacgcgatc
     3901 cccttcggaa cgttgaaggc gacgatcgcc tcggccaccg ggtccacggc cgggccgagg
     3961 tcggtgaacg cctccgcggt gcccgtcccg atcttgctct tcgccgggca cgccgagccg
     4021 ttggtgctct gaatccgctg atcggtcgcc ttgcactgcg ggatcgcctt ggtgttgatc
     4081 ttcgtgccgg cggggaagac gacggtcacc tcgcgggtcg cgatcggctt gccatcgggt
     4141 ccagttggcg tgcccgcgat cagcacgttg agtccggtcg acgtggccgg cttcgtggtc
     4201 gtggtcttgg cggtgaacgt cacgccgtcg acggtctcgg cagccgccac gccgaccgcc
     4261 gttgcagcca gcgcgcatgc gagcgcgccg gcaagcggct tcctgcgcat tgagtcctcc
     4321 tctaaggcca ggaaaggaac agacggcgca cccatccgga tgcgccgccg ccaccctaac
     4381 cgttatcgcc tgcacgggcc agtcgccgtg acgtggatgt cggcgtggcc ctcgtagccg
     4441 aacgtcgccg gcagcttcca cttgcccgac ttcgggcagg tcttcggcgt ggtgccatag
     4501 gccctcttgc ccgtgccggt cttcttgacc gcgaagtcga actggatgag cacggcattg
     4561 ctgccgcccg ggatcggaaa caacggcacg tcgaccacga gcttcggcgc ggtcgtgccg
     4621 ctgaaagtac ctgtgagcac cgcggtctgg ccgacggcgc cctttggcgt gagcaggaag
     4681 aagatcttct tcttgccgtt gaacgcctgg acgtcctcgg agaggggatc gactccctcg
     4741 ccgaggccgg tgttcgcctt cacgagcccg gttccgaccc tgctcttcga gcagacgttg
     4801 ccattggtgt tctggatcgt ctgcggtgag gccgtgcatt gcggcagcgc cttcgtgttg
     4861 atcttcgtcc cgggcgggaa ctgcaccgtg accttgcgga tcggcacctg cttgccgtcc
     4921 gcaccgacag gcgcgccctt gatcgtcgtg ctcagcccag tcggcgtgtt caccttcggc
     4981 gtggtcgtct tcgcgtccca ggtcacgcca tcgctcgtgc cgggagtcgc gatggcgacg
     5041 cccgcgacag ccgcgacaac gacacccgcg accgcgccgg cgatcggctt catgcccatg
     5101 cgagtctcct tcaggttggc caggaaagcg ccaacctaac cgcggtcgtg cgcgcaaagc
     5161 aaagcggccg cgctttcgcg cggccgcttg caacgtcacg tgtacgttct gtttaccgct
     5221 tgcaggagct gcgcgacttg attgtcgcgg tggtgccgtt ctcgtagttg aacttggtcg
     5281 cggtcgtcca gttcttcgag gcggggcacg tcgacggcgt ggtcaccagg ttcttcttct
     5341 tgctgcccct gcccgacgag atcgacttga gggcgacgtt gaacaccgtg agtgaggcgt
     5401 tgcctgcgcc cggtatcggg aaggccggca ccgtaaccgt cacgacgttc ttcctgacct
     5461 tgccgcggat cgtggcggtc tggccaaccg cgccctttgg cgtcagcaag aaaatgatct
     5521 ggctcttgcc ggcgaaggcg atgatgttct cgcccacggg gtcgatcccg gcgccgaggc
     5581 ctgtgatcgc ctccgccgag ccggtaccga gacggctgcc ggccggacac gcgttcgcgc
     5641 cctggctggc gaactggctg tccgtagccg tgcacacctt cgccgccgag gtgtcgacct
     5701 tcgtgccctt ggcaagcgtc acggtgacct tggtggccgg cgacggcttg ccgttagggg
     5761 cgcccgggtc ctgggccttc agcgagaccg ccacccccgt gttcgacctg ggcttcttgc
     5821 tggagtaccg gatggaggcc gtctgcttga actgcgggct tgcgatcgcc acggcagtca
     5881 cggcgagggc gagtacgccc acgacggcgc tgataagaag cttcttgctg cgcactgcgt
     5941 tactcctcct gagcagggaa aacgaaggca gggagaatcc ccgccggaat cccgtgaaca
     6001 cggggatggg gccagagttg cgctgttgtt accgggatcc ggccgctata gacggatctc
     6061 agccgagatg gcgcgctcga cgccgtcgag cagatcccag ttgccctcga gcgcgccgag
     6121 ggcgagctcg agcaggcggc tgtcgtcgca gtgccgacaa ttggcctcga tgaggcggcg
     6181 cgcctgcgcg gcgtgctcct cgtcgcggcg cgagtgcacc gcgaagtagg aggctgcgtc
     6241 gtcgtccgcc gctacgccgt agtgcgctgc gaggccgtcg agcttcgtct gcgagatcgc
     6301 cggctgcgcc gattcgatcg cgtagagcac ggccaggcgc tcctcctgcg agtcgccggc
     6361 cgtccacgcc gcgacgcagc gctcggtctc cgccgaggcc gcggcgtcgg tcgcgccgcc
     6421 gagcgagtgc acgaagcggt cccacagctc gacgtgcgcc tcctcctcgc gcgcgtgcag
     6481 ctcgagctcg gtgcgcgcgg cggggtcgtc cgccgcgtct gccgccgcgc ggctctccgc
     6541 ggctagcgcg accaccgcgt gccggtactg ccccgcgtac agcgcgagct cctccctact
     6601 gagctctcca gcgctccagc gcgtgtagaa gggatggctc agcacgtcgt agcggccgcg
     6661 ctcggcctcg agtcgctcga agaactccat ttgctcgctc ctcttcggaa atcgccccag
     6721 cagacaggtt gcgccatcct accggcgtgc gcgctccggc cgacctccag atccatgcgc
     6781 ccgagttccc gccgcggctc ccgtggctga acgtcgcctt cctgcggatg gacaagcagc
     6841 tcgggcagca cgccgtgctc gtggagttct gggacttcgc ccggatcaac tcgctgcgca
     6901 cgctgcccta catgaaggcc tggcacgagc gttacgccga cgccggcctg cgcgtggtcg
     6961 gggtccacac gccgggctac tccttcgggc gcgaccagga cgcggtcggg ctcgctgtct
     7021 ctcggctcgg aatcgactat ccggtcgcga tcgactcgga ctacctggtg tggcaggagt
     7081 acggcaccca gggctggccg tcgcgctacc tgttcgaccg ccgcggcatc ctgcgctacg
     7141 tgcactacgg cgagggcgat tacctggacg ccgagctcgc gatccaggag gtgctcgagg
     7201 agctcgatca ggacgcggtg ttcccgccgc cgctcgagcc gctccgcccc gaggacgagc
     7261 ccggcgcgtt gcttacgccg cagaccgcgg acatcgcgct gccggccgac agggatcgag
     7321 tggcgctcgt gcgcgactgg atcgatggcc cggactacat cgaggccgcc gacgccggtg
     7381 cctccgcgac cgcgtcgttc accgccggag gagcgtgggc ggtgctgtcg ggcgacggcg
     7441 ttgagccggg cctctacgag acgggcggga ccatcgtcgc cgagtcgccc ggcctgcggt
     7501 tgcacggctt ccagttcacg ccgctgccgc ctgagtgaag ctcggccgac aggcggcgaa
     7561 ccgcagcggc gtaggcgagc gaaaaccgag gagtccaagg acgcagggcc gaagcttggc
     7621 gatagccaag cgagcgcccg aggacgcggg aattcgaagg tttgcagccg cctacgacgc
     7681 tgcggcgagg gccttgcgga tttccttcgg cagcatgaag cgcacgtctt cctggaccac
     7741 ctcgaactcc gagatgtccg tcgtgccgcg ctgccggaac cagtcgacga gttcctgaac
     7801 gagttcttcg ggcgcgctcg cgccggaggt gatgccgacg acgcgggcgc cctcgagcca
     7861 ctcgtcttcg agctgaccga cgttgtcgat caggtgcgag cgtgcgccgt ggtcgcgcgc
     7921 gacctccacg aggcggttcg agttcgacga gttgcgtgag ccgatcacca gcaccagatc
     7981 gcactcgctg gccatctggc ggacggccat ctgccggttc gtggtcgcgt agcagatgtc
     8041 gtcggtgcgc ggaccgacga tgttcgggaa gcgctcccgc agccgaacga tgatctcgcg
     8101 cgtctcgtct accgacagcg tcgtctgcga gatataggcg atgtgctcgg gatccggtac
     8161 ctcgagcgcg tcgacgtcct ccacgctctc cacgagcacg atgttgccgg gcgcctcacc
     8221 catcgtgccc tcgacctcct cgtggccaga gtgcccgatg agcacgatcg tgtagtcctg
     8281 ggcggcgaac ttgcgcgcct ccacatgcac cttcgtcacg agcgggcagg tggcgtcgat
     8341 cgtctgcagc cggcgagccg ccgcgttctc gtgcacgctc ggcgcgacgc cgtgcgcgga
     8401 gaacacaacc gtctggccct cggggacctc cgtctcctcg tcgacgaaga tcgccccgcg
     8461 ctggcggagc tggtcgacca cgtgcttgtt gtggacgatc tccttgcgga cgtagacggg
     8521 cgcgccgtag agctcgaggg cgcgctcgac cgtctggacc gcccgatcga ctccggcgca
     8581 atagccgcgc ggagccgcca gcagcagctt ctgtggggct tcagccactg attcagagtg
     8641 tacgcaggag ctgggcgacc gactcgccca ccgcgtcgag gctcttgggc gacaccgccg
     8701 agaggttgtc gcaggtgcgg tggaagcagg cgaagtccca gtcgatcaag tcgatcgaag
     8761 gcacgccctc gctcaggaac ggggtgtggt cgtccgtgat ccgggcctcg ctcgcgagcg
     8821 ggaaggcgcc aatcgctcct acccggcgag ccgccgagcg gagcttgccc catagcctcg
     8881 tgttcgagct ccgctctcgc ggcaggcgga gctcgcggtc gccaacgaag tcgaagagga
     8941 tcatcgccgc ggcgtgggga tagcgcgcgg cggccacccg gctgccgcgc aacccgtact
     9001 gctcgaactg cgagtcgggg gtgccggcgg gactctcctc cccgtcgaag agggcgaaga
     9061 ccacggtcgg ccgagcctca cgcggcttca gccgcccagc aagctcgagc acgaccgcgg
     9121 tccccgaggc cccgtcgttc gcaccgagga agcccgggat gtccttggtg tcgtagtggg
     9181 cgcccactac cacgattcgg tggggatcct tgcccggcac ggagccgatc acgttgcgca
     9241 gcccgcccgg gacggcctga aagcgcccat gcggcagctt gcgtcgcagt gtctcggcga
     9301 gacgcctcga tgactcggaa ccggccggcc gtgggccgaa gctgagctgc agcttcagca
     9361 gccgccaggc gcgcccggcg tcaaagcggt cggccttgag gcgagccggg actgggtctg
     9421 gagagccgtg cttgcccggg aacggcaggc tgttggtggc cgcgagcgtg gcgaccgtcg
     9481 cgcccagcac gagctggacc gcaagcactg cgaacaggat ccgcacgcgg agattgtggc
     9541 ggttcacggg cgcttgtcgc tgaggcccgc ccagtcggac tcgcgctccg cgagcagcgc
     9601 gtcgatcccg tcgcgctcga gcgggcgggc gaggtagaac ccctgcccct tgtcgcagcg
     9661 gagatcgcgc agccgctcga gctgctcggc gtgctcgatg ccctccgcga ctgtctcgag
     9721 gccgagcgtc gcgcccagcg cgatcatcgc ggatgcgacc gccgcaccct cctcgccatc
     9781 ggccacgccg tgtgatcggt gcaagcgcgc gttctttgag ccgcttgtgg ggccttgtca
     9841 cccgcaatag gctcccttca aatgccgaca gcgcacatgg accggctgac cgcactcgac
     9901 gcgtcgtttc ttgctcagga gcgcaagagt tcgcacatgc acgttggcgc cgtgctcctg
     9961 ttcgaggggc cgccgccgca ccgagcggaa ttcaccgagc aggtggagtc acggctgcac
    10021 ctggtgccgc gctaccggca gaagctggcg ttcccgccac tcgagctggg acgcccattg
    10081 tggatcgacg atccgcgctt caacatcgag taccacgtgc gccacaccgc gctgccgtcg
    10141 cccgggtcgc tcgaccagtt gcgccagctc gcagggcgca tcttctccca gcgcctcgac
    10201 cgcgagaagc cgctgtggga gatctggctc gcgcaggggc tgaccgagaa ccgctgggcg
    10261 ctgatcacca agacgcacca cgcgatgatc gacggcgtcg ccggggtcga cctggcctcg
    10321 gtgctgttcg acctcgaccc cgtgccgcag ccgccctccg agccggccga gcagtgggcg
    10381 ccgcaccccg agccgtcgtc ggcggatctc gcggcggagg gcatcaaggg cctggtgaag
    10441 cggccgttca gcctcgcggg ccgcgccatt ggcgccgcca cgaagccagc ggagaccgcg
    10501 cgcgaggtgc tcgaggctgc cgaggcagtg ggcgaggttg cctgggcggg gctgaacccc
    10561 gcgccggaga cgccgctgaa cgtggacatc ggaccgcatc gccgtgtgtg gtgggtggag
    10621 agccagctct ccgacttcaa ggaggtcaag aacacgctcg gcgggaccgt gaacgacgtg
    10681 gtgctcgcgg tcgtgaccgg tgcgcttcgg cgctggctgc gccagcgcgg cgtgcgcacc
    10741 gagggcctcg agctgcgagc cctggtcccg gtgtcgatcc ggtcggaggc cgagcgcggc
    10801 gacctcggca accgcatcgc ggcgatgcgc gggccgctgc ccgtctactg cgaggacgcc
    10861 gtgcagtgcc tcgagatcgt cagcgaggcg atggcaaacc tcaaggagtc gaagcaggcg
    10921 ctcggcgccg aggtgatcgc cgggcttcag gacttcgcgc cgcccacgct gctcgcgcag
    10981 gcatcgcggc tgaacttctc cacgcgcctc ttcaacatga tcgtgaccaa cgtgcccggc
    11041 ccgcagttcc cgctctacat gctcgggcgc gagctgcagg acctgatccc catcgcgttc
    11101 ctgcccgagg gtcacgccct ggcgatcgcg atcatgagct acaacggcaa gatcacgttc
    11161 ggcctgctcg cggactacga cgcgatgccg gatctcgacg acttcgccat gtacctcgag
    11221 gactcgctcg gcgagctgct agacgcgtcg gcccgcgcca agcgccggaa gcaagacgcc
    11281 gctgagccga agcccgacgc cgccaaggcg ccgtcctcga gctgacaacc taaagaagtg
    11341 acgcacgcgt catctagacg cgttacgatg cgccgcggct gggaggccca atcggttcat
    11401 gaaccgctcc gttcttgcac tcctcctggc catggcggct gctgcgcttc ttgcgccagt
    11461 cgcctatgcg tcagacgtgc agcacctgca cttccggtac ggcccgatca acgtgaagcc
    11521 ggggtcgaac cagatccttg cgacaggcgg cgtcgagaag ccgtcgcagg acggctacat
    11581 cacgaggatc gtgccgaatc tgaagcgagc cgacggcagc attcccggca cgcagctgct
    11641 gcatctccac cacggcgtgt ggctcaacgc ctcgcggcgc gatccaacca cgccggcctt
    11701 cccgttcgag aagttcttcg cggcgggcga ggagaagacg tcgatgaacc tgccggcgcc
    11761 gtacggctat cccgtgaagg cgtcggacgt ctggatcctc aactacatga tccacgacct
    11821 cgtgacgagg ccgttcaagg tctacatcac ctacgacgtc gacttcgtgc cggcgcactc
    11881 cgccctcggc aagaagatga agccggtacg gcccatctgg atggacgtgc agaacgacca
    11941 cgcctacccg gtcttcgacg tgcacaaggg cagcggccgc aacggtcgct tcacctaccc
    12001 ggaccaggac ccgggtgcct accccgccgg tacgccaccg ctcaacgagt ggacggccga
    12061 ccgctccacc acgctcgtgg caatgacgat gggccacgtt catcccggcg ggctgtggga
    12121 cgacctcgac gtggttcgcg ccggagccca accgaacacg gcgaagggag tgattcccgg
    12181 ccacgtgccg cattcggtcc gaatcttccg ctcgaaggcg aagtactacg acccgaacgg
    12241 gccgatctcg tgggacgtgt cgctgcccgt ctcgaagcct tcgtggcgcg tgcacctgaa
    12301 caagggcgac aagctgcgca tcagcaccac gtacgactcg aagcacgcgt cgtggtacga
    12361 ggcgatgggg atcatgctcg cctaccagac cgaaggcgac gacggcggcc ccgatccctt
    12421 taggaccgcc gtcgacacga agggccggct cacccacggg cacctgaagg agaacgacgt
    12481 gtacggcggg cgcatgccgt cgctgccgaa cgcgaagaag ctgcccggcc ggttcgtgaa
    12541 aggcaacgag gtgcagatcc agggctacaa gttccttccg ggcgacttgc gcagcaggct
    12601 cggcaagcca tacgtccccg aggtgaggca ggggcagagc ctgcggttcg tgaacggcga
    12661 cgggccgccc gggcttggct tcctcaagca ggtctcgcac tcgatcacgt cgtgcgcgtt
    12721 cccctgcaat ctgagcactg gcatcagcta cccaatcgcc ggcggcagcg gcttcgactc
    12781 gggtcagatg ggcttcggcc gccccggctt caccgcgttc aacaacaggc agacgtggca
    12841 gtcgcccacg aacctgaagc cggggacgta cacgttcttc tgccggatcc acccgttcat
    12901 gcggggagcg ttccgggtcg tcggtcaata gcgccgccta gccactattc gccggcgagg
    12961 atccggcgct ttggggcacc tcgagctggc acggcctgcc gtagccggac cggagtccgg
    13021 cgctttttaa ttgctcacgc aacgggtact accctcgatg cgtgggcaag gtgcgcgtca
    13081 cacacatcac cgatcccggc tgcccgtggg cgtattccgc ggagccggcg gtcaacgcgc
    13141 tgcgcttccg ctacggaaac cagctcgagt ggcgtaccgt gatgatcggg ctcaccgagt
    13201 cgggcaagga gtacgaggtt cgcggctaca cgcccgagct gatggcggcc acgccgctcc
    13261 ggttcgagcg ctacgggatg ccgttcaccg ccggcctcgc gcggacgcgt gtgccgggga
    13321 ccggccgggc ctgccgagcc gtcgtggccg cgcgtctgat cgacccagat ctcggcgaga
    13381 tcgccctgcg agcgctgcgg ttcgcgtggt tcaacaccga tctgcttctc gacgaggacg
    13441 cgaacatcgc tgaagcgctg tcgcgcgcgc cgcagctcga cgccagggcg atcatgtcgc
    13501 gcatcgaatc gcccgatgtg gaggcggcct atcagcagga ccgcgcgctg gcgcgtagcg
    13561 ccgaaggcac agcggcgcac atgcagggga agactgcgcg gaccgacggg ccggagcgct
    13621 acaccgctcc gtcgctcatc ttcgaggcgg acgggcagcg gctgatcgcc ggcggatggc
    13681 agccgctctc ggcctacgac gtgctcatcg cgaacctcga tcccaccctg tccagacggc
    13741 cgcccgccag cgatcctctc gaggcgctcg cggagttccc gtacgcgctc acgacgcgag
    13801 agatcgcaac gctgatggcc gagcgcgacg acgagccgga tgacgcggcc gcgggcgagg
    13861 agctcgtgcg tctggccgcg cgccgccagg tggagcgcgt gccgctcggc aacgatggcc
    13921 tgtggcggcg ggtcgccgct gtggccgcag cggccgccta ggttccgggc gcgggatctt
    13981 cgttcggcgg acgcaggggc caaccggtga tcggcgggtt ggctgcgcgc tcgatcagtg
    14041 tcgcgacctc atctggctgg gactgcatcg gcacgtggct cgtgcccgcg acgatcacag
    14101 tcacggcgtt gatgcgctcg gccatccatc gctgcgtggc gggatgcacg acctggtcgg
    14161 agtcggcgat caggtagtac gagcgcacgt ccttccaggc ggggtggccg acggtgtcgg
    14221 tggaaaaggc ggtattcgca gtcggaccct gagccgtcgc catgatctgc gcggtcaccg
    14281 ggtccacgtc cggcgcgacc acccgcgcga agtgcgctcg gtcgacgatg aaggtgccgt
    14341 cgtcgccggg gacgatgtag ggctcccacc ccgcgggctg ggggtagcgg gccatgaggt
    14401 ccgccacgct ctcgccttcg tcgggcacga aggcggcgac gaagacgagc gccttcacgt
    14461 tctccgctcc gcgcgccgcc tcgcaaacca cggctccgcc gtagccgtga ccgacgacga
    14521 tgcacgggct cggcatctga tcgatctcgc aacgcacgac cgcggcgtct tgctcgacgc
    14581 cggtgagtgg cagttgggcc gcgaccactt cgtggcggga gcgctggagc gtgaagatca
    14641 cctcgcccca gctcgagccg tcgagcagcg ctccgtgcac taagacgatt tgagccatcc
    14701 aagcctccgc cggacgctat ccccggggct ttgcgcatgc aaccgcgatg atgtgggagt
    14761 gcaagctgtt tccctgcgaa acctgtgcct ctcgatgcca ggcgcggtcg aggagttccc
    14821 gttcggcccc cgcacgtcgg tgttcaaagt cggcgggaag atgttcgcgc tgagcgcgtt
    14881 ggacgcgcgg ccgctgagcg tgtcactcaa atgcgagccg gagctcgccg agcagttgcg
    14941 cctcagctat gcggcgatca cgcccggcta ccacctgaac aagcgtcact ggaacacggt
    15001 cgagctcgac ggctcggtgc ccgacaagat ggtgcgcgac atggtggagg acagctacga
    15061 cctggtggtc gacgggctgc cggcgagtaa gcgaaggacg atctccgcga cctgatcggg
    15121 ctcgcgagtg agtcttcggt acgtgatgtg gatgacgcgg tagcccacga gcgtgaggac
    15181 ggcgtcgcgc tcccgccctc gttcgaaggc cccgcggccg cggtggtacg cgtagccgtc
    15241 gagctcaacg atcacgcgct ctttctccca cagcatgtcc acctcaacgc cctcgacgat
    15301 tgcgttgacg gccggccgcg ggatgccgta ctcgtggcag acgccgagga acaggcgctc
    15361 gagctcgctg cgcgtgagcg gtggctcttc ggcgtcggtg aggagcgcgc gtaagacctt
    15421 cactccacgt cgaccagggc tgcgctcgca tacgtcctct atctcgcgga ggtcgaagat
    15481 ccccaggcga tcgccttcct cgattgctct ttggacttgg cggggcttca cgacctcagc
    15541 gaggttgagg tatgtgcggg ccaggctggt gacagggatg ccgtccacct cgtcggtatc
    15601 cctcggatcg agggcgcgga ctcggtggac ttgaatcccg cgctggccgg ctcgcccgcg
    15661 cggggttgtc acgtcgatga cgcgtctcga cgtcgcccgc acttcccaga gatcgccggc
    15721 gtggcggtgg ctgagccgtg catccgggcc acacgccagc accgcggccc tctcccagcc
    15781 gcgcttcggg agcgcttggt ggccgacggc atagacgccg cggaagaggc agtgaagccg
    15841 ggcggtagcc acccaatggt cgatcgtccc ccggccaatc ccgcgctcga acagttgcca
    15901 gcgaccaaca actccgtgct gctgcgaggc cagtgttgcg accacgcggg tacctgggcc
    15961 gttttcacca tgactgtcaa tgtcaaaacg gcccagggcg gtcatgcggg cgagcatggc
    16021 cgcagttgtg taacggcagg gttacgagcg tgacgattcg gccgtcagga ccggcgggcc
    16081 tggcctacgg cggcgggtgc tcgcccgcgt cgggctcttc gtccggctcc gcgtcggatg
    16141 gacttccgcg cggcgcgagg cccggctgga cgcgcgtccc gggctcgtcg gtgtgcggcg
    16201 caagcccggg ctcgacgagc ggctcgggcg gcggtccggg cgggcgcgcg ggcgggccgc
    16261 ctccgtcgcc atcgccaccg ccactgccga ggatcttcat gaacggcccg agcaggtcca
    16321 ccggcagcgg gaacacgatc gtcgagctct ggcccgagcc gagctcgagc agcgtctgca
    16381 ggtagcggag ctgcagcgtc gccgggttgc ggctgatgat ctccgccgcc tcgtagagcc
    16441 gctgcgccgc ctggtactcg ccctccgcgt tgatgatctt cgcgcgccgc tcgcgctcgg
    16501 cctcggcctg tcgcgccatc gcccgctgca tctgcgccgg gatctcgacg tccttgatct
    16561 cgacggttgt gaccttcact ccccacggct cggtctggtc atcgatgatc gcctgcagac
    16621 tctgattcag ccgttcgcgc tccgacagca gcgagtcgag gtcggccgtg ccgagcacgg
    16681 agcgcagcgt ggtctgcgcg atctgcgagg tcgcgaccag gaagttctcc acctcgacga
    16741 tggccttgtt cggatcgatc acccggaagt aagcgaccgc gttcacgcgc gccgtcacgt
    16801 tgtcgcgcgt gatcacctcc tgcggcggga tgttgagcgt caccgttcgc aggtcgacgc
    16861 ggaccatccg gtcgatgatc gggatcagga agatgagccc ggggcccttc tgggcgatca
    16921 ggcggccgag gcggaagatc acgccgcgct cgtactcgcg caggacgcgg atcgaggagg
    16981 ccaggatgat gaccgccagg aagacgacca ccgcgatgat cgctaagacg actgccacgc
    17041 cggctcccct ccttcgattt ccttcccctc gagatccgcc ggagcggcgg acacgaacag
    17101 cgtgaggccg tcgaccgagt cgacccgcac cttctcgccg atcgcgaagc tagcggagtc
    17161 gtcggacgcg cgcgcccgcc aaagcgcacc ctgcacgaac acctggcccg ccgggttgat
    17221 cggtacgcgt acctcgccga tcctgccgac gagttcctcc cagccggtgt gcaccctccg
    17281 ccgccgcgcc tcgagcgcct tcgagaccgc gaagagcgtc aggccgccga gggccgccgc
    17341 cgtgaagatc acgacgggaa ccgaaatctc gaatgccgag ttgtcggtgt tgaacagcag
    17401 cagcccgccc gcggcgagcg cgatgacccc cgcaatgccc agcaccccgt ggctcgtgat
    17461 cttcagctcc aagacgaaca gcactagcgc gaccagcaac agcacgacgc cggccgcatt
    17521 gaccggcaac tgcgacgtgc cgaacagccc gagcaccagc gccacggcgc cgagcgcgcc
    17581 cggcgcgacg atgcccgggt tgaacagctc gaacgcgatg cccgcgaagc ccgccgtgag
    17641 cagcaggtag gcgatcgtcg ggttcacgag cagctccagc aggtcgtact ggaacggcat
    17701 gtcgcgcttc acgatcgaga ggttcgccgt atggagcgtc tgcgccttcg ggcccttcac
    17761 cttgaagccg tcgagctggc gcagcaggtc ctgctgattg gtcgccacga tgtcgatcag
    17821 gtgctggtcg agcgcctccg acgcaggcac gttgaccgcg ctgcggacca tcttctccgc
    17881 caggtctgcg ttccggccgt gggcgcccgc gagcgcgcgc acgtaggcgg cggcgtcgtt
    17941 gcggatcttg cgcccgagcg tcttgccgat gtcctgcccg ttgccgttga tcggcgtggc
    18001 ggagccgatg ttggtctgcg gcgccatggc tgccacgtcg gccgactcgg tgatgtacag
    18061 cccggccgag gcggcccgag aaccctccgg gtacacgtac acgaccaccg gcatcggcgc
    18121 ggcgatgatg tgcttgatca tgtcgcgcat cgagctgtcg agcccgcccg gggtgtccat
    18181 ccggatgatc gcgatcgtgg cgcgtttgtc ggcggcctcc ttgagcgcct tgtcgatcca
    18241 cttctgcgtg gcggggtcga tcgtcgcgtt cagctcgatc gagtaggcga tcggcgcctt
    18301 accggtgctg ggcgtcgcgc cgcccgactg gccgaccgcg ctacccgcgg caacccacat
    18361 cagcagcccc agcacgatga gaagcgccgg aaccctcatg ccctaattat ggacggcggc
    18421 gtcacggccg cccacacgtc agacccgctc gggcatccag tcgagcggcg taagcgagcc
    18481 gttcttgccg ttcgccttca cgccgcgcag gcaaaagtcg gcagccgcga gcagctcatc
    18541 gaggtcgtcg gcgtcgtgcg ggtagcgcgc ccagccgacg ctcgcagtga tcgacagccc
    18601 cccgaactcg cccgggagcc gctcgtgcgc gcgccgaacg gcgtcgagca cacgctcggc
    18661 gagcgctcgc atcccgccct cgtcggcgcc gttcgccacg atcgcgaact cgtcgccacc
    18721 gaggcgagcg acgaggtcgt ccgcgcgggc tgcctccctc agcgcggtcg ccgcctcacg
    18781 cagcacgaga tctccaccgg ggtgaccgtg cagcgtgttg gtctccttga agtcatcgag
    18841 atccacaagc agcagccccg tggcgtcgct cgcgcgtccg ccgcctatgc ggacgctcat
    18901 acgctcctcg aacgcgcgcc ggttcgccag gccggtgaga tggtcggtga gcgagagcgt
    18961 gctcgcggca tcgcgtagtc gggtgatgtg cgccttgccg acagcgatca ccgagccgag
    19021 cacgacgtag gccggcgccg cgccccagat cagctggtac gccccgtccg ccacggcgtc
    19081 ctcctgatac agcagcggtg cggccaccac gaggagtgca atgccgatgt agacggccac
    19141 caggcgcggc ggatagaagc tcgccacgta ggcgacgatg aacatcaggt agtagttcgc
    19201 gaacgagtcg gtcccgccag tcgcccagac gatcgcgacg acgaaggcga agctcaggat
    19261 ggtgggcagg tggaagaccg ccgcgccgta gcgccatccg gcggtcaccc tgttccaggg
    19321 gaagaggaca cagaacgctc cccacgcgac ctcgagcacg cagaggatcc cgacgacggg
    19381 catgttcgcg tcgacatgcg gaagaagagc cgcgccggcg aggctggtta ccgcagcgac
    19441 gagccaaagc acgccggcaa cccgcagcgc gagcaggcgt tcctcctcct tggggatgcg
    19501 tacgttggcg atcttcactg cgcctcccct gcattgatcg gcaggggggc gccttcacat
    19561 gaggacgctt agaagccgag agcgaacttg gcttcttcgc tcatgaggtc cggcgtccag
    19621 ggcggcgaga agaccatgcg cggcctgacc tcgcggacgc cgtcgagctc gccgacgtac
    19681 tcctgcatct gctcgctgac ctgcggtccg atcgggcagc cgggcgaggt gagcgtgaac
    19741 gtgatgtcga catcaccgtc ctggtcgacg gagatgtcgt agacaagccc gagctcgatg
    19801 aagtcgagcc ccagctcggg atcgatcacg ttcgtgatcg cctcctggac gtcctcgacg
    19861 gtgacctctg ccatgccggt gatcttagta gcctggactc gtgccgcttt tcaagctgct
    19921 ggtggtcttc atcgtcgttc cgctcgcgga gctctacgtg atctacaaga tcggcgacgc
    19981 gatcgggatc ctccccacga ttgccctgct ggcgctggat tccgtcgccg gctcgctgct
    20041 cctgaagtcc caggggcgcg ctgtctggac gaagttccgg caagccctcg aacagggccg
    20101 cattccgcag cgcgaggtgc tcgacggcgt actcgtgatc ttcggcggcg ccttcctcat
    20161 cacccccggc ttcctcaccg acatcatcg
//
LOCUS       DASVXS010000180         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_660155, whole genome shotgun sequence.
ACCESSION   DASVXS010000180 DASVXS010000000
VERSION     DASVXS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660155"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VF752_10085"
     CDS             complement(<1..721)
                     /locus_tag="VF752_10085"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX8855940.1"
                     /translation="MDNRQIADRLDAFATLLELSDANPYTVRAYRRAAETIRSAVVPV
                     AELVRSGRVRSLRGIGPGIEARLRELVETGEIAELAELERELAPDLIGLGRYLGLSAK
                     RSLELARALDVRTAGELREAAAEGRLRSVPGIGPKIEAQLLEALASEAEPRPRQGLLL
                     NRAWELVGGIAAALDGEAAGDVRRWRDSCERLAVVCAAPDPGPVLARFEELPQIVALV
                     EQNEHHAVGVTVEGVPVELATA"
     gene            complement(734..1897)
                     /locus_tag="VF752_10090"
     CDS             complement(734..1897)
                     /locus_tag="VF752_10090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8855941.1"
                     /translation="MPEVAVAGTDLGLTDEQQDFVRSVRDFCARECTPEKVRELLAGE
                     DGHHSDEIARRMADLGWYGLCIPEEYGGSGGTFFDAVLFLEEWMRGEAPIGAYGVTLI
                     VVGALNRFGTEEQKRDLLGRVAQGGSLAIAMSEPDSGSDVASLRTQARRDDGEWVLNG
                     TKMWCSYAHKASHTLIVCRTEKGDNPHQGMSMVLVPRDAEGFTITPIDTMGGRETNEL
                     HLDNVRVPEDALLGIEGNGWTQLMAGLNNERVILAAMALGLAQRTFDQTLGYVKERKQ
                     FGRPVGSFQALQHRLADLATELTQVRLLVRWVAKLTDEDPGRMLPQEASMAKLAATEL
                     AKKAALEGVQMMGGYGYATEYPMERYLRSAVVTTIYGGTSEIQKNIIAKTLGL"
     gene            complement(1890..>2801)
                     /locus_tag="VF752_10095"
     CDS             complement(1890..>2801)
                     /locus_tag="VF752_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8855942.1"
                     /translation="TGNMEILAEHGTEAQRERWLQPLLDGEIRSCFSMTEPDSAGSDP
                     TGLATLAELDGDEWVINGHKWFTSGAVGASLAIAMVVTDPDAAPHKRASMILVPTDAP
                     GFNLIRPVPVMGHDGGPGHCEIVYDDCRVPADNLLGERGAGFKIAQDRLGPGRIHHCM
                     RAIGGAQRAYEMMCRRASEREAFGGPLGDKQFVQDFIAKSRMEIHAARLMTLHAAWKM
                     DTAGKREARQEISMIKVVAANMFMDVLDRAIQVHGALGVSDDTPIALMWRQGRMLRLA
                     DGPDEVHKMVIAARELNKYRAQEPVSA"
BASE COUNT          388 a         1023 c          958 g          432 t
ORIGIN      
        1 gggcggtcgc gagctcgacc ggcacgcctt cgacggttac ccccaccgcg tgatgctcgt
       61 tctgctcgac gagtgcgacg atctgcggga gctcttcaaa gcgcgcgagc acggggccgg
      121 gatcgggcgc ggcgcagacg accgccagcc gctcgcacga gtcacgccag cggcgcacgt
      181 cgccggccgc ctcgccgtcg agcgccgcgg caatgccccc gaccagctcc caggcgcggt
      241 tcaacaggag cccctgccgc ggacgcggct cggcttcgct ggcgagcgcc tcgagcagct
      301 gagcctcgat cttgggcccg attcccggca cgcttcgaag ccggccctcg gccgccgcct
      361 cgcgaagctc gccggccgtg cgcacgtcaa gcgcgcgcgc gagctcgagc gagcgcttcg
      421 cgctgagccc gaggtaacgg cccagcccga tgagatcggg cgcgagctcg cgctcgagct
      481 cggccagctc ggcgatctcg cccgtctcga cgagctcgcg cagccgggcc tcgatccccg
      541 ggcctatccc gcgcagcgat cgcacgcgcc cggaccgcac cagctcggcg acggggacta
      601 ccgcgctacg gatcgtctcg gcggcgcggc ggtaggcccg gaccgtgtac ggattggcgt
      661 cactcagctc gagcagcgtg gcgaatgcgt ccaggcgatc ggcgatctgg cggttgtcca
      721 ccgaccgagc ggctcagagc cccagcgtct tggcgatgat gttcttctgg atctccgagg
      781 tgccgccgta gatggtggtg accacggccg agcgcaggta gcgctccatc gggtactccg
      841 tcgcgtagcc gtagccgccc atcatctgga cgccctcgag cgcggccttc ttcgcgagct
      901 ccgtggcggc gagtttcgcc atcgaggcct cctgcggcag catccggccc ggatcctcgt
      961 cggtcagctt cgccacccag cgcacgagca ggcgcacctg cgtgagctcg gtcgccaggt
     1021 cggcgagccg gtgctggagc gcctggaagg agccgaccgg acgcccgaac tgcttgcgct
     1081 ccttcacgta gccgagcgtc tgatcgaacg tccgctgcgc gaggccgagc gccatcgcgg
     1141 cgaggatcac gcgctcgttg ttgagcccgg ccatcagctg agtccagccg ttgccctcaa
     1201 tgccgagcag cgcgtcctcc ggcacgcgca cgttgtcgag gtgcagctcg ttcgtctcgc
     1261 gcccacccat cgtgtcgatc ggcgtgatcg tgaagccctc ggcgtcgcgc ggcacgagca
     1321 ccatcgacat gccctggtgc gggttgtcgc ccttctcggt gcggcacacg atcagcgtgt
     1381 ggctggcctt gtgcgcgtac gagcaccaca tcttcgtgcc gttcagcacc cactcgccgt
     1441 cgtcgcgccg cgcctgcgtg cgaagcgacg ccacgtccga gcccgagtcg ggctccgaca
     1501 ttgcgatagc gagcgagcca ccctgagcga cgcggccgag caggtcgcgc ttctgttcct
     1561 ccgtgccgaa gcggttcagc gcgccgacca cgatcagcgt cacgccgtag gcgccgatcg
     1621 gagcctcgcc gcgcatccat tcctcgagga acagcacggc gtcgaagaag gtgcctcccg
     1681 agcccccgta ctcctccggg atgcagagcc cgtaccagcc gagatcggcc atgcggcgcg
     1741 cgatctcgtc ggagtggtgg ccgtcctcac ccgcgagcag ctcgcggacc ttctcgggcg
     1801 tgcactcgcg ggcgcagaag tcgcgcacgg agcggacgaa gtcctgctgc tcgtcggtga
     1861 gaccgaggtc ggtcccggca accgcgacct caggcactga ccggctcctg cgcgcggtac
     1921 ttgttcagct cgcgcgcggc gatgaccatc ttgtgcacct cgtcggggcc gtcggccagc
     1981 cgcagcatgc ggccctggcg ccacatcagc gcgatcggcg tgtcgtcgga cacgccgagg
     2041 gcgccgtgca cctgaatcgc gcggtccagc acgtccatga acatgttggc cgccacgacc
     2101 ttgatcatcg agatctcctg gcgcgcctcg cgcttgcccg ccgtgtccat cttccaggcg
     2161 gcgtggagcg tcatcaggcg ggccgcgtgg atctccatgc gcgacttggc gatgaagtcc
     2221 tgaacgaact gcttgtcgcc gagcgggccg ccgaacgcct cgcgctcgct cgcgcgacgg
     2281 cacatcatct cgtaggcgcg ctgggcaccg ccgatcgcgc gcatgcagtg gtggatgcgg
     2341 cccgggccga gccggtcctg cgcgatcttg aagccggcgc cgcgctcacc cagcaggttg
     2401 tcggccggca cgcggcagtc gtcgtagacg atctcgcagt ggccgggacc gccgtcgtgg
     2461 cccatcaccg gcaccggccg gatcaggttg aagccgggcg cgtcggtggg cacgaggatc
     2521 atgctcgccc gcttgtgcgg ggcggcgtcg gggtcggtga ccaccatcgc gatcgccagc
     2581 gacgcgccca ccgcgcccga ggtgaaccac ttgtggccgt tgatcaccca ctcgtcgccg
     2641 tcgagctcgg caagcgtggc aagaccggtg gggtccgagc ccgcgctgtc cggctcggtc
     2701 atcgagaagc acgagcggat ctcgccgtcg agcagcggct gaagccagcg ctctcgctgc
     2761 gcctcggtgc cgtgctcggc gaggatctcc atgttgccgg t
//
LOCUS       DASVXS010000181         8520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_873223, whole genome shotgun sequence.
ACCESSION   DASVXS010000181 DASVXS010000000
VERSION     DASVXS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873223"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF752_10100"
     CDS             complement(<1..405)
                     /locus_tag="VF752_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855943.1"
                     /translation="MFTVKVSAVSSAAPERVLGAARDFSERRPKVWRNVKAGHVEVHE
                     SGPDFADVTERLSIARSFWERGRYEWSQPDSVKQTVTDSNVLEPGSTWELRAAARDGG
                     GSDVEMVLQRNFRSGLRGSVGNLMNHLGGKRGW"
     gene            720..1148
                     /locus_tag="VF752_10105"
     CDS             720..1148
                     /locus_tag="VF752_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855944.1"
                     /translation="MPVEDVTSRGEPAEFVHGSGFAMCNEVPVELLKIAKIEPARAAE
                     RCVRNRDGTPRLQHVAYAVPPAMLEAVRADFEASRLPEYLSARFGDDVDMSYHDAGAT
                     LGHDAELHADSEGLRGFFAMVREAAASWDGSDLLRPPPGD"
     gene            complement(1319..1678)
                     /locus_tag="VF752_10110"
     CDS             complement(1319..1678)
                     /locus_tag="VF752_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8855945.1"
                     /translation="MATTPKRTPEPTSDACARGDAALARAFEFLGKRWNGVVLGNLSE
                     GPAGFRELSRAIGGISDSVLSDRLSDLSQAGLIARTVDEGPPLSVSYALTDRGKALIP
                     ALEQISLWAQEHLPGDD"
     gene            1774..2886
                     /locus_tag="VF752_10115"
     CDS             1774..2886
                     /locus_tag="VF752_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8855946.1"
                     /translation="MNVSPTVRPSVVVVGGGYAGINVAKALDDVGDVTLVDPTEAFVH
                     NVAAWRTLVEPEWLGRIFLAYDHLLAHGRFVRGRAVAVDGRRVTLESGRTLEPDYLVL
                     ATGSSYPFPAKTEEPDVASARTRLRAAHDALVAADRALIIGAGPAGLELAGEIKSFFP
                     EKKVTLMDMSEEILTGPYDQPLRDELHRQLAGLGIEVRLGSALNTLPSAPPATLAPIR
                     VRSEAGEEFVADIWFRAFGVRPHSEYVDGGSLSDRRDERGYLRVDEQLHVVGETNVYA
                     VGDVADADRDMAGIATRQAEVVAANIRASITGEGEPANYETFPPLIAIPLGPERGAGF
                     VGDGVADAATIAELKGRHMLIDTYASLFDAVPDVAA"
     gene            complement(3053..3125)
                     /locus_tag="VF752_10120"
     tRNA            complement(3053..3125)
                     /locus_tag="VF752_10120"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3090..3092),aa:His,seq:gtg)
     gene            complement(3157..3432)
                     /locus_tag="VF752_10125"
     CDS             complement(3157..3432)
                     /locus_tag="VF752_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855947.1"
                     /translation="MSSEAKNLEVISKAIDHHNRTCEYPAVAIAMNPFEVERLGWDSI
                     RGLPIREDPKLGTGAFRVICAKDEGDVREEAEEAVEAVSEHYVPVGQ"
     gene            complement(3452..4396)
                     /locus_tag="VF752_10130"
     CDS             complement(3452..4396)
                     /locus_tag="VF752_10130"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX8855948.1"
                     /translation="MSTCVVTGGAGFLGSHLCDRLLERGHRVICVDNLDTGNLNNIAH
                     VRDEKFDFRMLDITSHYEIPEPVDFVYHMASPASPIDYARLPLHTLKVGAYGTHNSLG
                     LAKAKRARFLLASTSEVYGDPQVHPQPETYWGNVNPIGPRGVYDEAKRYAEALTMAYL
                     RQQGVDTAIVRIFNTYGSRMRPHDGRAVPTFLRQALTDKPLTVFGDGSQTRSFCYVDD
                     LVEGLILLAESGVHEPVNIGNPNEMSLLEMAKTVIEVTGSRSEIVFEALPIDDPQVRQ
                     PDITKARDLLGWEPKVGLREGLEKTVQHMTETLGKLPA"
     gene            complement(4468..4614)
                     /locus_tag="VF752_10135"
     CDS             complement(4468..4614)
                     /locus_tag="VF752_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855949.1"
                     /translation="MCDHLYDNTSRYDADRELLTFLLVCPVCKIEKVVQTVEYAPKFT
                     PVAA"
     gene            complement(4766..4839)
                     /locus_tag="VF752_10140"
     tRNA            complement(4766..4839)
                     /locus_tag="VF752_10140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4803..4805),aa:Arg,seq:tct)
     gene            complement(4885..5088)
                     /locus_tag="VF752_10145"
     CDS             complement(4885..5088)
                     /locus_tag="VF752_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855950.1"
                     /translation="MRATTIRFSPAAWELIEREAQREGVSVAQYVREATLFHVAYRLG
                     ERGEGLADVRSLLHEELRHANFH"
     gene            5200..6240
                     /locus_tag="VF752_10150"
     CDS             5200..6240
                     /locus_tag="VF752_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8855951.1"
                     /translation="MAEAAVPSRRRRISRGAILKRILAVTVAGLALYGVAPKLGQVLG
                     DWPRLRDIKPGWFAVMAATESTSLVCMCAVQRIATQEHRWAPFFHSYLVGNAVSELVP
                     GGAATSAALQYEMILHEGVPPGRAASGLTAGSLIIFGTLLLLNVLTLPLVVLGVSVPT
                     TLMAAAWISVVLLLGIVLIGALALRGDRFLTGVGRAAQWIVNKVRRPSPPVDDLPKRL
                     LAQRTAVAMAVGGHWWQALLAAAGKWVFDYLTLVFALAAVGSRPNVALVLAAYSAASL
                     LGQIPLTPGGLGVVEAGLTGTLALIGVDGGAAVLATLAYRLFNYWLYLPAGLVALILH
                     RRSAHSRGAAAV"
     gene            complement(6237..7328)
                     /locus_tag="VF752_10155"
     CDS             complement(6237..7328)
                     /locus_tag="VF752_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8855952.1"
                     /translation="MRVVITVVVAAIALYLVYRLRKPLAWLFLAGFIALAASAPVAAL
                     QKWMRRGYAIALVYLAILLIPVGLLALFVPPIVTQGNNLVTNAPKYANDVTKWVNKNK
                     TLRKLDAKYDLTGKLEEQATKLTGRVGDVAGVLADIGSGIISSIFAGVTIFTLSLFMI
                     GGGPRWRRLLMRGHPPARAEALERLFDRINDAVGGYVRGALLQALIAGFSAWIVLLIL
                     GVPYAAPLAVVVFVFDLIPLVGATLAAVLVGLVTLFHNFPTPTIVWAIWSVVYQQIEN
                     NVIQPRIQSRTVRVEPFIVIVSVLFGGTLFGIFGAVLAVPIAASIQIAVTEYLRYRAD
                     TAVEIVKAGEPPPGGLPPTFPQRDAEPEP"
     gene            7583..7984
                     /locus_tag="VF752_10160"
     CDS             7583..7984
                     /locus_tag="VF752_10160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8855953.1"
                     /translation="MPFTLRNLKEDLEDVGSRFDGAPDLEFRLATDTLELEKSGLSYQ
                     RVPPGYRFPYGHTHKRQEEVYVVVRGSGRMKVDDEIVELKEWDAVRVPPGTWRGYEAG
                     PDGLEILVIGAPHLGEARREDVDGQRDWWAD"
     gene            complement(8033..8107)
                     /locus_tag="VF752_10165"
     tRNA            complement(8033..8107)
                     /locus_tag="VF752_10165"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8070..8072),aa:Pro,seq:tgg)
     gene            8209..>8520
                     /locus_tag="VF752_10170"
     CDS             8209..>8520
                     /locus_tag="VF752_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8855954.1"
                     /translation="MPPSRNRAFRIAAPVAAVAALATAPAGAQASNCVATASNPAVAS
                     ISRANNATVCLINQVRQQHGLRALHVSHRLSRAARRHSRSMVAHRYFEHGDYLGRIRE
                     TG"
BASE COUNT         1358 a         2895 c         2896 g         1371 t
ORIGIN      
        1 ccaccctcgc ttgccgccga ggtggttcat gaggttgccg acgcttccgc gtaggccgct
       61 gcggaagttg cgctggagaa ccatctcgac gtcgctgccg ccgccgtcgc gcgcggcggc
      121 acgcagctcc cacgtgctgc ccggctcgag gacgttcgag tcggtcaccg tctgcttcac
      181 cgagtctggc tgtgaccact catatcggcc gcgctcccag aacgaacgcg caatcgagag
      241 ccgctctgtg acgtcggcga aatcgggccc gctctcgtgc acctcgacgt gaccggcctt
      301 aacgttccgc cacaccttcg ggcgccgctc ggagaaatcg cgggcggcgc cgagcacccg
      361 ctctggagca gccgagctaa ccgcggagac cttgaccgtg aacacgcggc gagcttattc
      421 cagctccgcc gcgcgccgcc gagcgcgtcg gcgtcttgac caaggacctc cggtcgcggt
      481 cggcgagcgc aaggcggtcg gcggccagta gggtcggatc atcgacatcg ccgccgagct
      541 cgcggccgca cggtcgcggc ggtcgctgac caacccgaag aagcgccgca tgcccgagtc
      601 cagacatccc gacctcggcc agatccacca cctcgcctac gtcgtcgagg agatcgagcc
      661 ggcagcccgg cgcctccacg agcagttcgg gcgccggccc cttcttctac ctcgaggatg
      721 tgccggtcga ggacgtcacc tcgcgcggtg aaccggctga gttcgttcat ggttcaggct
      781 tcgcgatgtg caacgaggtg ccggtcgagc tgctgaagat cgccaagatc gagccggcgc
      841 gggccgccga gcgctgtgtg cgcaaccgtg acggcacgcc gcgcttacag cacgtcgcct
      901 acgcggttcc gcccgcaatg ctcgaggcgg tgcgcgcgga cttcgaggcg agtcgactgc
      961 cggagtacct cagcgcccgc ttcggcgacg acgtcgacat gagctaccac gacgcggggg
     1021 cgacgctcgg ccacgacgcc gagctccacg ccgacagcga gggcctgcgc ggcttcttcg
     1081 cgatggtgcg cgaggcggcc gcgagctggg acggctccga cctgctgcgc ccgccgcctg
     1141 gcgactagcg gcgctcgtcc gggcgcgccg aagatctcag ggtaaccccg aggccacggt
     1201 ccgtggccag gtacgcgact ctcccgccgc cgaaccccac ctgcaggcga cagattccta
     1261 tgcgaacggc gtcctcgacc tgtcctacgg cccagccgca tagggcgctt cggcccgctc
     1321 agtcgtcgcc gggcagatgc tcctgcgccc agagcgagat ctgctcgagc gcggggatga
     1381 gcgccttgcc gcggtcggtg agcgcgtaag acactgaaag aggcgggcct tcgtccactg
     1441 tgcgggcgat cagccccgcc tgagagaggt cggacaaccg gtcggaaagg accgagtctg
     1501 agataccgcc aatcgcgcgc gagagctcgc ggaacccggc cgggccctca ctcaggttgc
     1561 cgagcaccac gccattccag cgcttgccaa ggaactcgaa tgcgcgcgcg agtgcagcat
     1621 cgccacgggc gcaggcatcg gaggtcggtt cgggcgtccg cttcggcgtt gtggccacgg
     1681 attcaatgct agcgtcgcgc tggttgctag tgttttcgaa gtcgctttct gcggcaatgt
     1741 cgatacttga accagagcaa gggagatcct tccgtgaacg tcagtccgac cgtccgtccc
     1801 tccgtcgttg tcgtcggtgg cggctacgcg ggcatcaacg tcgcgaaagc gcttgacgac
     1861 gtcggcgacg tcacgctcgt cgaccccacc gaggctttcg tccacaacgt cgcggcgtgg
     1921 cgcacgctgg tcgaaccgga gtggctcggt cgcatctttc tcgcgtacga ccacctgctc
     1981 gcgcatggcc ggttcgtccg tggtcgcgca gtcgcggtcg acgggcgccg ggtcacgctg
     2041 gagtcagggc ggacactcga gccggactac ctggtgctcg ccaccggctc gtcctatccg
     2101 ttcccggcca aaacggagga gcccgacgtc gcctccgccc ggacacgcct tcgggcggcg
     2161 cacgacgcac ttgtggccgc cgaccgggcg ctgatcatcg gcgcggggcc cgccggcctg
     2221 gagctagccg gcgagatcaa gtcgttcttc cccgagaaga aggtgacgct tatggacatg
     2281 tctgaggaga tcctcacggg cccctacgac cagccgctcc gcgacgagct gcaccggcag
     2341 cttgccggtc tcggcattga ggtacggctc ggcagcgcgc tgaacacgct gccgagcgcc
     2401 ccaccggcaa cgctcgcgcc gatccgagtc cgcagcgagg ccggcgagga gttcgtcgcc
     2461 gacatctggt tccgggcctt cggtgtgcgg ccgcacagcg agtacgtcga cggcggatca
     2521 ctgagtgacc gccgcgacga gcgcggctac ctgcgcgtgg atgagcagct gcatgttgta
     2581 ggcgagacca atgtctacgc ggtcggcgac gtcgccgatg ccgaccgtga catggcaggt
     2641 atcgctacga ggcaggccga ggtggtcgca gccaatatcc gcgcttcgat caccggcgag
     2701 ggcgagcccg ctaactatga gacgttccca ccgctcatcg cgatcccgct cggtcccgag
     2761 cgcggggcgg gcttcgtcgg cgacggtgtc gccgacgccg ccacgatcgc cgagctcaag
     2821 ggccggcaca tgctgatcga cacctacgcc tcgctcttcg atgcggtgcc ggacgtcgca
     2881 gcctgaggct gaggggtcgt tcgggtcacc ggttttgagt ggtcttagcg caccggatca
     2941 atgggtccca tcggacccac tgacaggccg ttgatgagaa acggcgcccc cgagcacaag
     3001 ctggaacgcc gtcggcgcta cttcttagaa cggctgatag aagaaaccgg agggtgagcg
     3061 aggggactcg aacccccgac cgcctggacc acaaccagga gctctaccag ctgagctacg
     3121 cccaccaggg agcgcgaagt ctagcggtgg gacgggctac tgcccgactg gcacgtagtg
     3181 ctcggagacc gcctcgacgg cctcctcggc ctcctcgcgg acgtcgccct cgtccttggc
     3241 acagatcacc cggaaggcac ccgtgccgag cttcgggtcc tcgcggatcg gaagcccgcg
     3301 gattgagtcc cagccgagcc gctcgacctc gaaggggttc atcgcaatcg caaccgcggg
     3361 gtactcgcag gtgcgattgt gatggtcgat cgccttcgag atcacctcga ggttcttggc
     3421 ctcggaactc acgaggccga gtgtatcgag gctacgcggg cagcttgccg agcgtttcgg
     3481 tcatgtgctg caccgtcttc tcgaggccct cgcggaggcc gaccttcggc tcccagccga
     3541 gcaggtcacg cgccttggtg atgtcgggct ggcgcacctg cgggtcgtcg atcggcagcg
     3601 cctcgaacac gatctcggag cgcgagccgg tcacctcgat caccgtcttc gccatctcga
     3661 gcagcgacat ctcgttggga ttgccgatgt tgaccggttc gtgcacgccg gactcggcga
     3721 gcaggatcag tccttcgacg aggtcgtcga cgtaacagaa gctacgcgtc tgcgagccgt
     3781 cgccgaagac agtgagcggc ttgtcggtaa gcgcctggcg caaaaacgtg ggcacggcgc
     3841 gaccgtcgtg cggcctcatt cggctcccgt aggtattaaa aatgcgaacg atcgccgtat
     3901 ctacgccctg ctgccgcagg tacgccatcg tgagcgcctc ggcgtagcgc ttcgcctcgt
     3961 cgtaaacgcc gcgcgggcca attgggttga cgttgcccca gtaggtctcg ggctgcgggt
     4021 gcacctgcgg atcgccgtag acctcggacg tcgaggcgag caggaagcgc gcgcgcttcg
     4081 ccttcgccag accgagcgag ttatgcgttc cgtacgcgcc gaccttcagc gtgtgcagcg
     4141 gcagccgcgc gtagtcgatc gggctcgccg gcgaggccat gtggtagacg aaatcgaccg
     4201 gctccgggat ctcgtagtgg cttgtgatgt cgagcatccg gaagtcgaac ttctcgtccc
     4261 ggacgtgggc gatgttgttg aggttgcccg tatcgaggtt gtcgacgcag atcaccctgt
     4321 gaccgcgttc gagcagccgg tcgcagagat gcgacccgag gaagccggca ccgccggtga
     4381 ccacgcaagt tgacatcggc gcgagaggct agcggaagcg tcggaagctg ccggtgcggc
     4441 gcttggcggg gcgccgacgg cgactgccta cgccgcgacc ggcgtgaact tgggcgcgta
     4501 ctcgacggtc tgcacgacct tctcgatctt gcacaccggg cagaccagga ggaacgtgag
     4561 cagctcacgg tcggcgtcgt agcgcgaggt gttgtcgtac aggtggtcac acatggtgct
     4621 ctcctaccag tggtcaaaca caggtgcact gttacgcgcg cgcacgcccg tctctatgcc
     4681 cctgctcccg agatcggctt ccgccccgcc ggagcgctgg gtcgggccag ccctacgtcg
     4741 ccgtcggggc ttcttcgtgt cctaacgccc ccggcaggat tcgaacctgc gacctaacgg
     4801 gtagaagccg tccgctctga tccactgagc tacgggggcc ggtggctcgt gcggagaata
     4861 gccccggtcc gggcgggcgg ccgctcagtg gaagttcgcg tggcggagct cttcgtggag
     4921 gaggctgcgg acgtccgcca acccctctcc ccgctcgccc agccggtaag ccacgtggaa
     4981 cagggtcgcc tcgcgcacgt actgggccac gctgacgccc tctctctgcg cctcgcgctc
     5041 gatgagctcc caggcggcgg gactgaatcg gatggtggtc gctcgcatct tcacgacccc
     5101 ttcaacgcgc gaacgtcgcg caggttgggc gatatcggaa gagacagccg ccgccgtctc
     5161 tgcgtcagcc gccgggcgcg cccgggacaa tgtgtcgccg tggccgaagc ggcggtaccg
     5221 agcaggcgcc ggcgaatctc ccgcggcgcc atcctgaaac ggatactcgc cgtcacggtg
     5281 gcggggctgg cgctctacgg ggtcgcgccc aagctgggtc aggtactcgg cgactggccg
     5341 cgcctgcgcg acatcaagcc gggctggttc gcggtgatgg ccgccacgga gagcacgagc
     5401 cttgtgtgca tgtgcgcagt gcagcggatc gcgacccagg agcatcgctg ggcaccgttc
     5461 ttccactcat acctggtcgg caacgccgtc agcgaactgg tgcccggagg ggctgccaca
     5521 tccgctgcac tccagtacga gatgatcttg cacgaggggg ttccgcccgg tcgcgccgca
     5581 tcggggctga cggcgggctc actgatcatc ttcggcacgc tgctgctgct aaacgtgctg
     5641 acgctgccgc tcgtggtgct tggcgtgagc gtaccgacga ctctcatggc ggcggcgtgg
     5701 atctccgtcg tgctgctcct cggcatcgtc ttgatcggag cgctcgcgct caggggcgac
     5761 cgcttcctca ccggggtcgg gcgggccgcg caatggatcg tcaacaaggt gcggaggccg
     5821 tccccgccgg tagacgatct tccgaagcgg ctcctcgccc agcgcactgc cgtcgccatg
     5881 gccgtgggcg gccactggtg gcaggcgttg ctcgcggcgg cgggaaagtg ggtgttcgat
     5941 tacctgacac tcgtcttcgc gcttgcggcg gtcggatcgc gccccaacgt cgcgctcgtg
     6001 ctggccgcgt actcggccgc gtcactgctc gggcagattc cgctcacgcc cggtgggctg
     6061 ggggtcgtgg aagccggcct gaccggcacg ctcgcgctta tcggcgtgga cggcggcgcg
     6121 gcagtgctcg ccactctcgc gtaccggctc ttcaactact ggctgtacct gcccgccggt
     6181 ctcgtggcgc tgatcctgca ccggcggtca gcgcactcac ggggagccgc cgcggtctag
     6241 ggctcgggct ccgcatcccg ctgcgggaac gtcggtggga ggcctcccgg tggaggctcg
     6301 cccgccttca cgatctcgac ggcggtgtcg gctcgatagc gcaggtactc ggtcaccgcg
     6361 atctgaatcg aggcggcgat cgggacggcg agcaccgcgc cgaagattcc gaacagcgtg
     6421 ccgccgaaga gcaccgacac gatcacgatg aacggctcga cgcgtaccgt gcgcgactgg
     6481 atgcgcggct ggatcacgtt gttctcgatc tgctgataga cgaccgacca gatcgcccag
     6541 acgatcgtcg gcgtcgggaa gttgtggaac agcgtgacga gcccgacaag gaccgccgcg
     6601 agcgtcgcgc ccacaagcgg gatgaggtcg aacacgaaca ccacgacggc gagcggtgcc
     6661 gcgtacggga ccccgaggat gagcagcacg atccacgccg agaatcccgc gatcagcgcc
     6721 tgtaggagcg cgccgcgcac atagccgccg acggcgtcgt tgatgcggtc gaagaggcgc
     6781 tccaacgcct cggcgcgtgc gggcgggtgc ccgcgcatca gaagtcttcg ccacctgggc
     6841 ccacctccaa tcatgaagag gctgagcgtg aagatcgtca ccccggcgaa gatcgagctg
     6901 atgatcccgg agccgatgtc ggccagcacg ccggcgacgt cgcccacccg gcccgtgagc
     6961 ttcgtcgcct gctcctcgag cttgccggtg aggtcgtact tggcgtcgag cttgcggagc
     7021 gtcttgttct tgttgaccca cttcgtcacg tcgttcgcgt acttgggcgc gttcgtcacg
     7081 aggttgttgc cctgcgtgac gatcggcggg acgaacagcg ccagcagccc gacggggatc
     7141 agcagaatcg ccaggtagac gagcgcgatc gcgtatcccc gccgcatcca cttctggagc
     7201 gcggcgacag gcgccgacgc ggcaagtgcg atgaagcccg cgaggaagag ccaggcgagt
     7261 ggcttgcgca gccggtacac gaggtacagc gcgatagccg cgaccaccac cgtgatgacg
     7321 acacgcacga tggaccgcca cgcgggcgcg tcacgcggcg gagccgcggg ctgcggtatg
     7381 gcacgcgtcg tcaaggtgag ctctccccgc ttggaattgg ccttgggatg gttgattcct
     7441 gcccggagac ggggcggcta gtcctctgtc cccacgtccg aagcggtggg gttgagcgag
     7501 ttcttcgggg ctacgtcagc gggtgaggcg cggcggagga tcagattggc gaccccagcc
     7561 aggggtgcca tagcattgcg ccgtgccgtt cacgctcagg aacctcaagg aggacctgga
     7621 ggatgtcggg tccaggttcg acggcgcgcc ggacctggag ttccgcttgg cgaccgacac
     7681 gctcgaactc gagaaatccg gcctgagcta ccagcgcgtc ccacccggct atcgctttcc
     7741 gtacggccac acgcacaaga ggcaggagga ggtctacgtg gtcgtccgcg ggagcgggag
     7801 gatgaaggtc gacgacgaga tcgtcgagct caaggaatgg gacgcggtgc gcgtcccgcc
     7861 cggtacgtgg cgaggctacg aggccgggcc ggacggcctc gagatcctcg tcatcggagc
     7921 cccccatctc ggcgaggctc ggcgcgaaga cgtcgatggg cagcgcgact ggtgggctga
     7981 ctagcgccag gtacggcgca cttttgcgct gagcgacgcg ctctcaacta gatcgggacg
     8041 gccggattcg aaccggcgac ctcctgctcc caaagcaggc gcgctaacca ggctgcgcta
     8101 cgtcccggcg cctggacgaa tgttcgaccc atcgaccggg ccgctcaagc ataggcgcgc
     8161 ggtggcgacc gaagacctgg accccacccg accctgccca ggtttcacat gccgccttca
     8221 cgaaaccgcg cattcaggat cgccgctcca gtcgccgccg tcgccgccct ggcaaccgcc
     8281 cctgcgggtg cccaggcctc gaactgcgtc gccaccgcct ccaatccggc ggtcgcgtcg
     8341 atctcccgcg cgaacaatgc aacggtctgc ctcatcaacc aggtgcgcca gcagcacggc
     8401 ctgcgcgcgc tccacgtgag ccaccggctg agccgcgccg ctcgccgcca ctcgcggagc
     8461 atggtcgcgc atcgttactt cgagcacggc gactacctcg gccgcatccg cgagacgggc
//
LOCUS       DASVXS010000182         9888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_873504, whole genome shotgun sequence.
ACCESSION   DASVXS010000182 DASVXS010000000
VERSION     DASVXS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873504"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..153)
                     /locus_tag="VF752_10175"
     CDS             complement(13..153)
                     /locus_tag="VF752_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855955.1"
                     /translation="MRVLAKVAYWVAVVILALALLVALVLFFESRDQSGLSGGVLPLL
                     RL"
     gene            complement(153..677)
                     /locus_tag="VF752_10180"
     CDS             complement(153..677)
                     /locus_tag="VF752_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8855956.1"
                     /translation="MRDEDFERIYSEHAEALFGFLVYRTGDRALAEDVLADTFERVLR
                     TRARFDPRKASEKTWVYSIALNRLKDLHRKSAAEGRAVERAGVDAAAFDPGAELAGVE
                     DREEIGRALAGLSAEEREAVALRFGADLTVPEIAKVLGEKLSTVEGRVYRALRKLRSA
                     LEEPDSARVARGGP"
     gene            755..1312
                     /locus_tag="VF752_10185"
     CDS             755..1312
                     /locus_tag="VF752_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855957.1"
                     /translation="MTSRNEEHVPADLLHVAERLRKDRPQPTALELDQIKLRARSQAA
                     RRAPSLFSRQKGFLMKSRLALTSVLVIGLLMSMTGAGLAVSGIASSGSAGVAQYGAPA
                     PTTPTTPTGGGGGEVLGNQETSPTSTSSPTTEAGAVSPARQVSAEQAGGLPFTGYLAI
                     PLLIVGIAMLATGLIVRRRTARDNS"
     gene            complement(1327..3318)
                     /locus_tag="VF752_10190"
     CDS             complement(1327..3318)
                     /locus_tag="VF752_10190"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8855958.1"
                     /translation="MSIARLPAAPLLLVPGALTVYLAFNAGGFFPAAPGWVAVGLAVL
                     LVLRITLAEEPFAGVSPWLVAALGFLGAYCVWVLLSGSWSDSPARALLEFNRDMAYLL
                     ALALFGSLAAGSRGVAWMLRGLVLGAFVVCAISLIARIAPDVWPITANISSQRLSYPI
                     TYWNALGILCALSIVFCVHLTSSEREPPIVRVLGAAAVPVFATTLYFTFSRGSIVTTA
                     IGLVLYLLIGRPRAMLTGLLAVAPVTAVAVVTAYGKDALSSIDPTTAQAVSEGRDLAL
                     VVALCVVGAAALRALLLFADRPLAEFRLPSGTRRPLGIALAVGVVALAVGVTAGFNLP
                     HRVSHQYDRFVHGNVVRDNADLRRRLSDPGNNGRIDVWRVARDEFDRSSFHGSGAGTY
                     ETLWNRYRPVPQAAKDGHSLYLETMGELGIVGLILIVGVLASIFGGLIVRRRGPDRAL
                     YSVILAAGLAWAIHAGVDWDWEMPVITLWLFALGGAAIGALPDPRAAERGRSETLGPP
                     RLLRVVAGVLVLVLAITPALVASSQASLDASVRAFKRGDCHKAADEALSSISAISARP
                     QPFEILAYCDVRVGLDRLAITQMKKAVKRDPDNWEFQYGLALVTGAAGRDPRPPMRTS
                     LALNPRDSLNQETARAFRKARTPRQWKRRALNARLPFQR"
     gene            3670..5175
                     /locus_tag="VF752_10195"
     CDS             3670..5175
                     /locus_tag="VF752_10195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8855959.1"
                     /translation="MIQIVMDGAVASGAHEEDFTGELTLAPRLIGAGFATRSSRGSER
                     WSVLRRLLLSADLIAATLAGTIVALATGLSSSDAFGFALAVALGWPLSAFVFGLYAAD
                     DLGSWASGVGEAPRMVVALLVLSWPLYAVAGAAGAPAPVTSALVAGAGTALLSASARA
                     GARAMIHRVAPLRQRTVIVGSGQVAGQLVRKLQIHEQFGLQPIGIVDDELHNIGTPDL
                     PHLGRLDDLERVLRLHAVDRVIIAFSRASHERLLKSIRACRDQRVAVDVVPRLFEFLD
                     GASRALGSIGGLPLLSITAPQMNAASRAAKRALDIFVSGSALLVLSPLLVVIAAAIKL
                     ESRGPVFFRQVRAGRGGNVFRVFKFRSMYADAEQRKVELHAVNDVSDGVMFKMHRDPR
                     VTRTGRVLRRLSLDEVPQLFNVLRGEMSLVGPRPLILPESQALEEDWHERRLDLRPGL
                     TGPWQVSGRSDIPFQEMVRFDYQYVAGWSLARDIEILLATIPAVLSGRGAY"
     gene            5222..6319
                     /locus_tag="VF752_10200"
     CDS             5222..6319
                     /locus_tag="VF752_10200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8855960.1"
                     /translation="MRVLQVSESFGGGVLEVVRTLSERLAAQGHEVTLAYGVRPETPE
                     DVRSAVSREVELFAMPWTKRSAGAQVAAVRQLRALVAEWKPDVVHLHSSFAGVVGVVA
                     VRDVPTVYSPHAYSFTRSNDAAPVRAAYRAVERFIARRCSVIGAVSESEASLARGALG
                     AERVEVVPNGIPELDVEPPLRMPDRRPPLVVAAGRIGPQRRPEACARILSAISGVAEV
                     RWLGKEPSEGSGTEALRAVGVAVSGWIPREEVMRALEEAVAYLHWTAWDGQPLSVLEA
                     MARDAIVVASDIPPNREILGPDQVCAREEQAADLLRRVITDDALRSSLLAAQHERRGS
                     YGAQRMVDDWLDLYRRIGSHVAEAAHARRSG"
     gene            6441..7793
                     /locus_tag="VF752_10205"
     CDS             6441..7793
                     /locus_tag="VF752_10205"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX8855961.1"
                     /translation="MELRDFVALAWKRRWYVLFMVALTVGLGAAFAATKQKQYEATAT
                     IAMTPNIGTGQGFVDPNALSAVIRTYQQTAQSEFNIRRAEQLANTKLPGKVSTSSEAG
                     AGILKITGRADTPTGAQTTAWFPTLAFMESLRKNKLLVAQLVDRPGKPTSPVQPRPPL
                     IIGVSLILGLGAGLLLAFALEQFRRRVETPQDLQDLTPAPVIGRLPRQRSLQRGEARP
                     VWEVPKMAILQESFRSLRTNIEFLTEGNHTAIQVTSATASQGKSTVVANLGIALAQIG
                     IETIVLDADLRRPVQHRIFGLENSRGLSTLMAIPGSKVRPLATHYPHLSVVPSGPVPP
                     NSTEMLHVRFHAVLEEIRSTGALVLIDSPPLLPVSDSRLIAPHADGVIVVVAAGSSKP
                     GTLRSAFEKLQFAEARVLGVVLNQSGEDADSGGEYDYYVYGEEPIKSSTQSGRAAIPQ
                     "
     gene            7790..9151
                     /locus_tag="VF752_10210"
     CDS             7790..9151
                     /locus_tag="VF752_10210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8855962.1"
                     /translation="MTARLLASRGILLPASLLALLLAYASARSPIKALFICLGVAAVA
                     LAVANVEWLLLALLAAFPWEGELHYPTETVTVVKLVGLLLIGAWLLRVVRRNETLRTS
                     PAVLPAALFLMFVTLSLLLSPDIGVGVSKYLRYVLFVVFFFLVLQLTRERKDVVRALR
                     VIALSSGVAAVIGLENFLFKGAGRAGGPIRDPNDFAYLMAAILPITAYLFSADKLRRP
                     LWGACFVVTFAAMLATLSRGAIVGIAALAVWAIVSRSVRIGGIVVGALTGVGVLLLSF
                     TLWSGVINEKLQSKSQIASANAQSREAFWDAAKRMWLDRPVTGVGPERFGELANQYVR
                     NNPITLDKPVVHNTYLELLAECGLFALLAFVGYLIAVWRQLASGRRRAREAGDEDGWR
                     LNTALQASLVVSIVSGVFLSEQLSVPFWLIGGLAATASVVVTRKSEEPAPAAPAEAAP
                     ATA"
     gene            9148..>9888
                     /locus_tag="VF752_10215"
     CDS             9148..>9888
                     /locus_tag="VF752_10215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8855963.1"
                     /translation="MRVVLVTSLERGGPVEHSLLLARELTRHGADVGAVCATPELRQR
                     FEAAGADAAVIPLRRTLDVSGARAAMRFCDGADVVHGQDRRSGFWLRLWPRARRSPGG
                     RAVRVCTIHGLPDPYLPPPVGTDRPALRDRLAYPLLDARLCRRADAIVVPSEAGAALL
                     ARIGYPRELMTVIPNGVEVHEETGPRAGGAVGTVSMLEPVKALDVFLRAAARVRAGRP
                     DTRFEVYGTGSDEQRLASLARELGLEDRL"
BASE COUNT         1462 a         3488 c         3443 g         1495 t
ORIGIN      
        1 cgcaccaccc gcctagaggc gaagcagcgg cagcacgccg ccgctcagcc cgctctggtc
       61 gcgcgactcg aagaacagca cgagcgctac gagcagcgcc agggcgagaa tcaccacggc
      121 cacccagtag gccaccttgg caagcacgcg catcaggggc cacctctcgc gaccctcgcg
      181 gaatcgggct cctcgagcgc cgagcgcagc ttgcggagcg cccggtacac gcggccctcg
      241 acggtcgaga gcttctcgcc gaggaccttg gcgatctccg gcacggtgag gtcggcgccg
      301 aagcgcagcg ccaccgcctc acgctcctcg gcgctcaacc cggcgagggc gcggccgatc
      361 tcctcccgat cctcgacccc cgcaagctcg gcgcccgggt cgaacgccgc cgcgtccacg
      421 cccgccctct ccaccgcccg cccttcggcg gccgacttgc ggtgaaggtc cttcagccgg
      481 ttcagcgcga tcgagtacac ccaggtcttc tcgctcgcct tgcgcgggtc gaagcgcgcg
      541 cgggtacgca gcacgcgctc gaacgtgtcc gcgagcacgt cttcggcgag cgcccggtct
      601 cccgtccggt agaccaggaa gccgaacagc gcctcggcgt gctcagagta gatgcgctcg
      661 aagtcctcgt cgcgcatgga cccaccatga tggcctttcg gaaggcggag cgtaggcagt
      721 aaggaatggg cggactcgtt cgtataagcc tgcgatgacc tctagaaacg aagagcacgt
      781 cccagccgac ctgcttcacg tggccgagag gctgcgcaag gatcggccgc agcccaccgc
      841 gctcgagctc gaccagatca agctccgcgc ccggtcgcag gcggcgcgtc gtgctccaag
      901 cctcttctca cgtcagaaag gattcctgat gaagtcacgc ctcgcgctga cctctgtcct
      961 cgtcatcggc ctgctgatga gcatgaccgg ggccggcctc gccgtgtccg gtatcgcctc
     1021 gagcggcagc gccggtgtcg cgcagtacgg tgcccccgcg cccaccacgc ccaccacccc
     1081 caccggcggc ggcggtggcg aagtgctcgg caaccaggag accagcccga ccagcacgag
     1141 ctcgccgacc accgaggccg gggccgtctc gcccgcgcgc caggtgtctg cggagcaggc
     1201 aggcgggctg ccgttcaccg gttacctcgc gatcccgctc ctgatcgtgg gcatcgcgat
     1261 gctggcgacc ggcctcatcg tccgccgccg cacggcacgc gacaacagct gagccctact
     1321 cgggccctag cgctggaatg ggaggcgggc gttcagcgcc cgcctcttcc attgccgcgg
     1381 cgtgcgcgcc ttgcggaatg cccgcgccgt ctcctggttg agcgagtcgc gagggttcag
     1441 cgccagcgac gtgcgcatcg gcgggcgcgg gtcgcggccc gcggcgcccg tcacgagcgc
     1501 aaggccgtac tggaactccc agttgtcggg gtcgcgcttc acggccttct tcatctgcgt
     1561 gatcgcgagc ctgtcgaggc cgacgcggac gtcgcagtag gcgaggatct cgaacggctg
     1621 tggtcgcgcg gagatggccg agatcgagga gagcgcctcg tcggcggcct tgtggcagtc
     1681 gccgcgcttg aaggcgcgca cgctcgcatc gaggctcgcc tgggatgagg ccacgagcgc
     1741 gggcgtgatc gccagcacga gcacgagcac gccggccacc acgcgcagca gccgcggagg
     1801 gcccaacgtc tccgagcgcc cgcgctccgc cgcgcgcggg tcgggcagtg cccctatcgc
     1861 cgcaccgccg agtgcgaaca gccacagggt gatgacgggc atctcccagt cccagtccac
     1921 accggcgtgg atcgcccacg cgagcccggc cgcgaggatc accgagtaga gcgcccggtc
     1981 ggggccgcgg cggcgcacga tcaagccgcc gaagatcgag gcgagcacgc cgacgatcag
     2041 gatcaggccg acgatcccga gctctcccat cgtctcgagg tagagcgagt gcccgtcctt
     2101 ggccgcctgc gggacgggcc ggtagcggtt ccacagcgtc tcgtatgtgc ccgcgcccga
     2161 cccgtggaag ctcgagcggt cgaactcgtc gcgcgccacg cgccacacat cgattcggcc
     2221 gttgttaccg gggtccgaga gccgccggcg caggtccgcg ttgtcgcgca cgacgttgcc
     2281 gtgcacgaag cggtcgtact ggtggctaac gcggtgcggc aggttgaacc cagcggtgac
     2341 gccgacggcg agtgcgacca cgccgactgc cagcgcgatg ccgagcggcc ggcgcgtacc
     2401 ggagggaaga cggaactcgg cgagtgggcg gtccgcgaac agcagcaggg cgcggagcgc
     2461 cgcggcgccc accacgcaca gcgcgacgac cagcgccagg tcgcgccctt cggacacggc
     2521 ctgcgcggtg gtcggatcga tcgacgagag agcgtccttc ccgtacgccg tcacgacggc
     2581 caccgccgtc acgggcgcga cagccaggag acccgtgagc atcgcgcgtg ggcgcccgat
     2641 cagcaggtag agcacgagcc cgatcgcggt ggtgacgatg cttccgcgcg agaacgtgaa
     2701 gtagagggtc gtcgcgaata ccggcactgc tgcggcgccg agcacgcgca cgatcggcgg
     2761 ctcgcgctcc gagctcgtga ggtgcacgca gaacacgatc gagagcgcgc agaggatgcc
     2821 gagtgcgttc cagtatgtga tcgggtagct gaggcgctga ctcgagatgt tcgccgtgat
     2881 cggccacacg tccggggcga tccgggcgat cagggaaatc gcgcaaacca cgaacgcgcc
     2941 gagcaccagg ccgcgcagca tccacgccac gccgcgagag ccggcggcga gcgagccgaa
     3001 cagcgcgagc gcgaggaggt acgccatgtc gcggttgaac tcgagcagcg cgcgggcagg
     3061 cgagtcggac cagctgcccg acagcagtac ccagacgcag tacgcgccga ggaacccgag
     3121 cgccgccacc agccacgggc tcacaccggc gaagggctcc tccgcgagcg tgatgcgcag
     3181 cacgagcagg accgcgagcc cgaccgccac ccagccggga gccgcgggga agaagccgcc
     3241 cgcgttgaac gcgaggtaga ccgtcagcgc gcccggcacg agcagcagcg gcgccgccgg
     3301 cagacgggcg atcgacatcc ggcggatcat agggtcggct tcattcgctg cacgtcagcg
     3361 atatcgcgaa cggcatctgt cagggatggc aatatccccg ttccggggtt atcttcaact
     3421 cgttggcacg gatgccttcg cacaacctgg ccctggtctc gcgcatgcac cggcgaccgg
     3481 ttcgcctccc tcgatgagct gcctgtttcg ctcgcgaaca cgcgtggcag ccgtggggcg
     3541 tgcccggtac ggcgggcgac ttgcgtctac ttgggttagt gcgccccgaa cggggtaggc
     3601 aaagagaagc cgactcgcag gggagcgtcg cggacgggcg ctcaagaccg catcacatac
     3661 tgccgaaggg tgatccagat agtcatggat ggcgctgtcg caagcggagc acacgaagag
     3721 gatttcaccg gcgagctgac ccttgccccg cggctgatcg gagcaggctt cgccacgcgg
     3781 agctcgcgcg gcagcgaacg ctggtcggtg ctgcgacgcc tgctgttgtc cgcggacctg
     3841 atcgcggcga cgctcgccgg cacgatcgtg gcgctcgcca cgggcctctc atcgagcgac
     3901 gccttcggct tcgcgctcgc ggtcgcgctc gggtggccgc tgtccgcgtt cgtgttcggc
     3961 ctctacgcgg ccgacgacct cgggtcgtgg gcgagcggcg tcggtgaggc gccgcgcatg
     4021 gtggtcgcgc tgctcgtgct ctcgtggccg ctctacgcgg tggccggcgc cgccggcgcg
     4081 cccgcccccg tgacgtctgc gcttgtggcc ggcgccggca cggcgctcct cagcgcctcg
     4141 gcgcgagcgg gcgcccgggc gatgatccat cgcgtcgcgc cgctccggca gcgtacggtg
     4201 atcgtcgggt cgggtcaggt cgccggacag ctcgtgcgca agctccagat ccacgagcag
     4261 ttcgggctgc agccgatcgg catcgtcgac gacgagctgc acaacatcgg cacgcccgac
     4321 ctcccgcacc tcggccgcct cgatgacctc gagagggtgc tgcgcctgca cgctgtcgac
     4381 cgtgtgatca tcgccttctc gcgcgcgagc cacgagcggc tgctgaagag catccgcgcg
     4441 tgccgtgacc agcgcgtagc ggtcgacgtg gtgccgcgcc tgttcgagtt cctcgacggg
     4501 gcctcgcgcg cgctcggctc gatcggcggg ctgccgttgc tctcgatcac cgccccgcag
     4561 atgaacgcag cctcgcgggc cgccaagcgc gcactcgaca tcttcgtctc cggcagcgcc
     4621 ctgctcgtcc tgtcgccgct gctcgtggtg atcgccgccg cgatcaagct cgagagccgc
     4681 gggcccgtct tcttccggca ggtccgcgcg ggacgcggcg gcaacgtctt ccgcgtgttc
     4741 aagttccgct cgatgtacgc ggacgcggaa cagcgcaaag tcgagcttca cgccgtgaac
     4801 gacgtctccg acggcgtgat gttcaagatg caccgcgacc cccgcgtcac gcggaccggc
     4861 cgcgtgctcc ggcgcctctc gctcgatgag gtgcctcagc tgttcaacgt gcttcgcggc
     4921 gaaatgagcc tcgtcggtcc gcgcccgctg atcctgcccg agagccaggc gctcgaggag
     4981 gactggcacg agcggcgcct cgacctgcgg cccggcctca cgggaccgtg gcaggtctcc
     5041 ggccgctcgg acattccctt ccaggagatg gtccgcttcg actaccagta cgtggccggc
     5101 tggtcgctcg cgcgtgacat cgaaatcctg ctcgccacga ttcccgcggt gctctcgggt
     5161 cgcggggctt actagcgggc ctccgtcgcc gcggtcgctc cggcgtgtag cgtccgcggc
     5221 catgcgagtc ctccaggttt ccgagtcgtt cggcggtggt gtcctcgaag tcgtccgcac
     5281 gctgtccgag cgcctcgccg cgcagggcca cgaggtcacg ctcgcgtacg gcgtccgccc
     5341 cgagaccccg gaggacgtgc gctccgccgt cagccgcgag gtcgagctct tcgcgatgcc
     5401 gtggacgaag cgcagcgccg gtgcgcaggt tgcggcggtg cggcagctgc gggcactcgt
     5461 cgcggagtgg aagcccgatg tcgtgcacct ccactcgtcc ttcgccggtg tggtgggcgt
     5521 cgtcgcggtg cgtgacgtgc ccaccgtgta ctcgccgcac gcctactcgt tcacgcgctc
     5581 gaacgacgca gcgccggtgc gcgccgcgta ccgggcggtc gagcgcttca tcgcgcggcg
     5641 ctgcagcgtg atcggagcgg tgtccgaaag cgaggccagc ctggcacgcg gcgcgctcgg
     5701 agctgagcga gtcgaggtcg tcccgaacgg gatcccggag ctcgacgtag agccgccgct
     5761 gaggatgccc gaccgccggc cgccgctcgt ggtcgcggca ggaagaattg gcccgcagcg
     5821 gaggccggaa gcgtgcgctc gaatcctctc ggcgatctcc ggcgtggctg aggttcggtg
     5881 gctcgggaag gagccgtcgg agggctccgg caccgaggcg cttcgcgccg taggcgtggc
     5941 ggtcagcggc tggataccgc gcgaggaggt catgcgcgcg ctcgaagagg ccgtggcgta
     6001 cctccactgg accgcgtggg acggccagcc gttgtcggtg ctggaggcga tggcccgcga
     6061 cgcgatcgtc gtcgcgagcg acatcccgcc gaaccgggag attctcggac ccgaccaggt
     6121 ctgcgcgagg gaggagcagg ccgctgacct gctgcgccgc gtgatcaccg acgatgcgct
     6181 gcgcagctca ctgctcgctg cccagcacga acggcgcggc tcgtacggcg cacagcggat
     6241 ggtcgacgat tggctagacc tataccggcg gatcgggagc cacgtagctg aggccgcgca
     6301 cgcgcgccgt tccggataaa gccgcaccgc ggatctgtcg atagcgagcc tgtagcaacc
     6361 agtcgacgtg gaaggcgcgc ggcagtactc tcgactgagc cgcgcgggga atccctcccc
     6421 aatcgtagaa tcaaggtcga atggaacttc gagacttcgt tgcgctcgcg tggaagcgcc
     6481 gctggtacgt cctgttcatg gtcgcgctca cggtcggtct cggagcggcc ttcgcggcta
     6541 ccaagcagaa gcagtacgag gccaccgcga ccatcgcgat gactccgaac atcggcacgg
     6601 gtcagggctt cgtcgacccg aatgccctct ccgcggtcat ccgcacctac cagcagaccg
     6661 cgcaatctga gttcaatatc cgtagggccg agcagctcgc caacaccaaa ttgcccggca
     6721 aggtgagcac ttcgagcgag gcgggcgcgg gcatcctcaa gatcaccggc agagccgaca
     6781 cgcccaccgg ggcgcagacg acggcgtggt tccccacgct cgcgttcatg gagtcgctgc
     6841 ggaagaacaa gcttctcgtc gcgcagctcg tcgaccgccc gggcaagccg acctcgcctg
     6901 tgcagccgcg gccgccgctg atcatcggcg tgtcgctcat cctcggcctc ggtgccggcc
     6961 tgctgctcgc gttcgcgctc gagcagttcc gccgccgcgt cgagacgccg caggacctgc
     7021 aggatctcac cccagcaccg gtcattggcc gcctgccgag gcagcgcagc ctgcagcgag
     7081 gtgaggcgcg gccggtgtgg gaggtgccga agatggcaat cctccaggag agcttccgct
     7141 cgctgcgcac gaacatcgag ttcctcaccg agggcaacca caccgcgata caggtcacga
     7201 gcgccacggc ctcgcagggc aagtccacgg tggtggcgaa cctcgggatc gccctcgcgc
     7261 agatcgggat cgagacgatc gtcctcgacg ccgacctgcg ccggccggtg cagcaccgca
     7321 ttttcgggct cgagaacagc cgcggccttt ccacgctgat ggcgatcccg ggcagcaagg
     7381 tgcgcccgct cgccacccac tacccgcacc tctcggtcgt accgagcggg cctgtgccgc
     7441 cgaactccac cgagatgctt cacgtgcgct tccacgccgt gctcgaggag attcgctcca
     7501 ccggtgcgct cgttctgatc gactcgccgc ctctgctgcc ggtcagcgat tcgcgcttga
     7561 tcgcgcctca cgcggacggc gtgatcgtgg tcgttgctgc tggctcgagc aagcccggca
     7621 cgctgcggag cgccttcgag aagcttcagt tcgccgaagc gcgcgtgctc ggcgtggtgc
     7681 tgaaccagtc cggtgaggac gcggacagcg gcggcgagta cgactactac gtctacggcg
     7741 aagagccgat caagagcagc acccagtcag gcagagcagc cataccgcaa tgaccgccag
     7801 gttgctggcc agcaggggaa tcctgctacc agcctcgctc ctcgcactcc tactcgccta
     7861 cgcctcggcg cggtcgccga taaaggcgct gttcatctgc ctgggcgtcg cggcagtggc
     7921 tctggcggtc gcgaacgtgg agtggctgct gctggccctg ctcgccgcct tcccgtggga
     7981 gggcgagctg cactatccca ccgagacggt cacggtggtg aagctggtgg gactgcttct
     8041 gatcggcgcc tggctgctgc gagtcgtccg ccgcaacgag acgctgcgga cctcccccgc
     8101 cgtgctgccc gccgcgctgt tcctcatgtt cgtgacgctc tcgctgctgc tctcccccga
     8161 catcggcgtg ggtgtctcga agtacctgcg gtacgtgctc ttcgtggtgt tcttcttcct
     8221 ggtgctgcag ctcacgcgag agcgcaagga cgtcgtccga gcgctccgcg tgattgcgct
     8281 gtcgagcggc gtcgcagcgg tgatcgggct cgagaacttc ctcttcaagg gcgccgggcg
     8341 cgccggcggc ccgatcaggg atccgaacga cttcgcctac ctgatggcgg cgatccttcc
     8401 gatcaccgcg tatctgttct cagcggacaa gctccgccgc ccgctgtggg gcgcctgctt
     8461 cgtggtcacg ttcgcggcga tgctcgcgac cctctcgcga ggcgcgatcg tgggaatcgc
     8521 cgcgcttgcc gtctgggcga tcgtctcgcg cagcgtgcgc atcggcggca tcgtggtcgg
     8581 cgcgctcacg ggcgtcggcg tgctgctcct ctcgttcacg ctctggtccg gggtgatcaa
     8641 cgagaagctc cagtcgaagt cgcagatcgc cagcgcgaat gcccagtcgc gcgaggcgtt
     8701 ctgggacgcc gcgaagcgca tgtggctcga ccggccggtg acgggcgtcg ggcctgagcg
     8761 attcggcgag ctcgcgaatc agtacgtccg caacaacccg atcacgctcg acaaaccggt
     8821 ggtgcacaac acctacctcg agctcctggc ggagtgcggc ctctttgcgc tgctcgcttt
     8881 cgtgggctat ctgatcgccg tctggcggca gctcgcgtcg ggcagacgcc gcgcccgcga
     8941 agccggggat gaggacggct ggcgtctgaa caccgcgctc caggcgtcgc tggtggtgtc
     9001 gatcgtctcc ggcgtgttcc tcagcgagca gctgagcgtg ccgttctggc tcatcggcgg
     9061 actcgccgcc accgcatcgg tggtggttac gaggaagagc gaagagccgg cgccggcggc
     9121 tcccgccgag gctgctccag caaccgcgtg agggtggtgc tcgtcacgtc gctcgagcgg
     9181 ggaggcccgg tcgagcactc gctcctgctg gcgcgggaac tcacgcgaca cggcgccgac
     9241 gtgggcgccg tgtgcgcgac gcccgagctg cggcagcgct tcgaggcggc cggtgccgac
     9301 gcagcggtga tcccgctcag gaggacactc gacgtgtccg gagcgcgggc cgcgatgcgg
     9361 ttctgtgatg gcgccgacgt ggtgcacggc caggatcgcc gcagcggctt ctggctccgc
     9421 ctctggccgc gcgcccggcg gtcgcccggc gggcgtgccg tgcgcgtctg cacgatccac
     9481 ggcctcccag atccgtacct tccgcctccc gtcggcaccg acaggcccgc gctgcgcgat
     9541 cgcctcgctt acccgctgct cgacgcgcgg ctttgccggc gggccgacgc gatcgtcgtc
     9601 ccgtccgagg cgggcgccgc gctgctcgcc cggatcggct atccgcgcga gctgatgacc
     9661 gtgattccga atggagtcga ggttcacgag gagacaggtc cacgcgcggg cggggccgtg
     9721 ggaacggtgt cgatgctcga gccggtgaag gcgctcgatg tgttcctgcg cgcggcggcg
     9781 cgcgtgcgag cgggccgtcc tgacactcgc ttcgaggtgt atggcacggg ctcggacgag
     9841 caacggctcg cttcgctggc gcgcgagctc gggctcgagg atcggctg
//
LOCUS       DASVXS010000183         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_237089, whole genome shotgun sequence.
ACCESSION   DASVXS010000183 DASVXS010000000
VERSION     DASVXS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237089"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF752_10220"
     CDS             <1..183
                     /locus_tag="VF752_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855964.1"
                     /translation="DHTRAYLHYLSRANELTAARLITLHNLTFMKLLMDGLREAIEQG
                     RYDEQARHVLAGEAPY"
     gene            complement(217..1401)
                     /locus_tag="VF752_10225"
     CDS             complement(217..1401)
                     /locus_tag="VF752_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8855965.1"
                     /translation="MATPSEIDGRYRIDSRLGAGGMSTVFKGTDTVLERPVAIKLLAE
                     HLADDEAFVARFRREALAAARLQHPNIVQVFDSGQDPESGRHYIVMEYVDGPSCADLL
                     RDHDQLEIGQAVSVVRDACHGLDYAHRAGVVHRDVKPGNLLISSETGAAKLADFGIAK
                     AAEQTRITQVGSVLGTAAYLSPEQARGEEAGPASDIYSLGVCAYQFLTGRLPHEYTSL
                     TELALKQQNNPVEPIRNSRPEVPPALDDAIRLCLRREPSERYASALDLATAVEAGLHG
                     ETTQATQLLQTGGWDPDATQALPATQATRAMPRGGTGYYEPTPAPTTQAPERRPAKQR
                     KTSGQRLARLALLLAVILAIVAIVLIVTQSGSKGVQPVNEGDVHGQIDGLKQLVQDHT
                     RP"
     gene            complement(1401..1940)
                     /locus_tag="VF752_10230"
     CDS             complement(1401..1940)
                     /locus_tag="VF752_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855966.1"
                     /translation="MTRRAAGLAAPLAAAALAFAGCGGDGVDNAKHGIPQSDAQAILT
                     QLDQIGSQFDNKACNGASAKVRELQTRVDGLPRSVDADLRRELVNGVRHLDQLVADQC
                     QRPEPTQTETTTTPSETTPPPTTETTPTETQPPPTQTEPPPTTTTPTPTTPGTGGTAP
                     GSGNGGDNSNGTGQGGTGD"
     gene            complement(1937..2710)
                     /locus_tag="VF752_10235"
     CDS             complement(1937..2710)
                     /locus_tag="VF752_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8855967.1"
                     /translation="MSLHSRETVESDKPAKIALLSDVHGNLPAFKAVLEDVERSGADT
                     IWCLGDLVGYGAQPDECVALARETCDVCLVGNHDLVVLGKLDIATFSANAAVAARWTQ
                     DQISNDTREFLAALESLDETGPLSIYHASPRDPVWEYVLSTLAAADCMDVMDARVGAI
                     GHSHVALWFHRDGEGSASGEVAQGGTKLDISDGEWLINPGGVGQPRDGDPRAAWLLLD
                     TGDWSADWRRVEYPIEEAAGAIEQAGLPQALATRLFVGQ"
     gene            2699..3943
                     /locus_tag="VF752_10240"
     CDS             2699..3943
                     /locus_tag="VF752_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8855968.1"
                     /translation="MEAHGVRVPLAVIGAAAAAGAATLLLRPRSGLIDPAPVRAEGYF
                     SEDELQRARAFRRPQRAIAFSGMGVSGATVLGLALRPPEVLSRLRDRPLAGAAATGAA
                     GSLFFTALGLPFSAVSHRRSVRFGLSTQSWRSWFSDLGKATALGTGFSAAGAALGMGL
                     VRRYPRRWWMPGSAAIVGVGGAFTFLSPLLLDPIFNKFTPLPEGPLRTEVLSLAEKAG
                     VDVGEVYRVDASRRTTGVNAYVWGLGRTKRVVLYDTLIDGFPADQVRSVVAHELSHVK
                     HRDVPRGLLWLAIVAPLGTLLVKEIANSLSDDSEPSPAMLPALALATSLVSFWGQVAG
                     NVLSRAVERRADAFALSLTDEPEPFIEMERALSVKNLGDPSPPRLMQLLFGTHPDTLE
                     RIGIGVAWGTRGGGAAPAEQQQ"
BASE COUNT          646 a         1629 c         1702 g          784 t
ORIGIN      
        1 gaccacaccc gcgcctacct tcactacctc tcacgcgcga acgagttgac cgcggcgcgg
       61 ctgatcacgc ttcacaacct cacgttcatg aagctgctga tggacggctt gcgcgaggcg
      121 atcgagcagg gccgctacga cgagcaggcg cgccacgtgc tcgccggcga ggctccgtac
      181 taggcgcgcg aacgcgcctc acggcctgcg cctcgcctac gggcgcgtgt ggtcctgcac
      241 cagctgcttc agcccgtcga tctggccgtg cacgtcaccc tcgttgacgg gctgcacgcc
      301 tttgctcccg ctttgggtca cgatcaggac gatcgcgact atcgcgagga tcaccgcgag
      361 caggagcgcc aggcgagcca ggcgctggcc cgaggtcttg cgctgcttgg ctggacggcg
      421 ctcgggcgcc tgcgtcgtcg gcgcgggcgt cggctcgtag tagccggtgc cgccgcgcgg
      481 catcgcgcgc gtggcctgcg tggcgggcag ggcctgcgtg gcgtcggggt cccaaccgcc
      541 ggtctgaagc agctgcgtcg cctgggtcgt ctcaccgtga aggcccgcct cgaccgcggt
      601 cgcgaggtcg agcgcgctcg cgtagcgctc gcttggctcg cgcctcaggc agagccggat
      661 cgcgtcgtcg agcgcgggcg gcacctccgg tcgggagttg cggatcggct cgaccgggtt
      721 gttctgctgc ttcagcgcga gctcggtgag cgaggtgtac tcgtgaggaa gccgtccggt
      781 gaggaactgg tacgcgcaca cgccgagcga gtagatgtcc gacgcgggcc cggcctcctc
      841 gccgcgcgcc tgctcgggtg acaggtacgc cgcggtgccg agcaccgagc cgacctgcgt
      901 gatgcgcgtc tgctcggccg ccttcgcaat accgaagtcg gccagcttcg cggcgccggt
      961 ctccgacgag atcaggaggt tgccgggctt cacgtcccgg tgcacgacgc cggcgcggtg
     1021 cgcgtagtcg agcccgtggc acgcgtcgcg cacgaccgag acggcctgcc cgatctcgag
     1081 ctggtcgtgg tcacggagca ggtcggcgca cgagggaccg tcgacgtact ccatgacgat
     1141 gtagtggcgc ccgctctccg ggtcctgccc cgagtcgaac acctgcacga tgttcgggtg
     1201 ctggagccgc gcagccgcga gcgcctcgcg ccggaagcgg gccacgaacg cctcgtcgtc
     1261 ggcgaggtgc tcggcgagca gcttgatcgc gacggggcgc tcgagcacag tgtcggtgcc
     1321 cttgaagacg gtggacatgc cgcccgcacc gagccggctg tcgatccggt agcgcccgtc
     1381 gatttccgag ggagtcgcca tcagtcgccc gtcccgccct gtccggtgcc attgctgttg
     1441 tcgccgccgt tgccgctccc gggagccgtc ccgccggtgc ccggcgtggt aggcgtggga
     1501 gtcgtggtcg tcggaggagg ctcagtctgg gtcggcggcg gctgagtctc cgttggcgtc
     1561 gtctccgtgg tcggcggcgg cgtcgtctcg ctcggggtgg tggtcgtctc cgtttgagtc
     1621 ggctcaggac gctggcactg atccgccacg agctggtcga ggtgccggac gccgtttacg
     1681 agctcgcgcc gcaggtccgc gtccacgctt cgcgggaggc cgtcgacccg ggtctgaagc
     1741 tcgcgaacct tcgcgctcgc gccgttgcac gccttgttgt cgaactggct gccgatctgg
     1801 tcgagttgcg tgaggatcgc ctgggcgtcg ctctgcggaa tgccgtgctt cgcgttgtcg
     1861 acgccatcgc cgccgcagcc cgcgaacgcg agtgctgctg ccgcgagcgg cgctgcgagt
     1921 cccgccgcgc gccgggtcat tggcccacga agagccgggt cgcgagggcc tggggaagcc
     1981 ccgcctgctc gatcgcgcct gccgcctctt cgatcgggta ctccacccgc cgccagtccg
     2041 cgctccagtc gccggtgtcg agcaggagcc aggcggcgcg cgggtcgccg tcgcgcggct
     2101 gacccactcc gcccgggttg atcagccact cgccgtccga gatgtcgagc ttcgtcccgc
     2161 cttgggcgac ctcgcccgac gccgacccct cgccgtcgcg gtgaaaccag agcgccacgt
     2221 gggaatggcc tatggcgccg actcgcgcgt ccatcacgtc catgcaatcc gcggcggcga
     2281 gcgtcgagag cacgtattcc cacacggggt cccgcgggct ggcgtggtag atgctgagcg
     2341 gcccggtctc gtccaaggac tcgagggcgg cgaggaactc gcgtgtgtcg ttgctgatct
     2401 ggtcctgggt ccagcgcgcg gcgacggcgg cgttggcgga gaaggttgcg atgtcgagtt
     2461 tgccgagcac gaccaggtcg tgatttccca cgaggcagac gtcacacgtc tccctcgcca
     2521 gcgcgacgca ctcgtccggc tgcgcgccat agccgacgag gtcacccagg caccagatcg
     2581 tgtcggcgcc tgagcgctcc acgtcctcga gaactgcctt gaaggcgggg aggttgccgt
     2641 gcacgtctga cagcagcgcg atcttcgcgg gcttgtcgct ctctacagtt tctcgcgaat
     2701 ggaggctcat ggcgttcgag tgccgctggc ggtgatcggc gcagcagccg cggctggtgc
     2761 ggcaacgctc ctgctgcgcc cccggagcgg cctgatcgac ccggcccccg tccgcgccga
     2821 agggtacttc agcgaggacg agctgcagcg tgcgcgggcc tttcggcgcc cgcagcgagc
     2881 gatcgccttc agcggtatgg gcgtgtctgg ggcgacggtt ctcggactag cactgaggcc
     2941 gcccgaggtg ttgtcccgcc tgcgtgaccg cccgcttgcc ggggcggctg cgaccggggc
     3001 cgcgggctcg ctcttcttca ctgctcttgg cctgccgttc tccgccgtgt cccaccggcg
     3061 ctccgttcgg ttcggtctct ccacccagag ctggcgcagc tggttctccg acctcggcaa
     3121 ggcgacggcg ctgggcaccg gcttcagcgc ggccggggct gcgctcggga tggggctcgt
     3181 gcggcgctac ccgcgcaggt ggtggatgcc gggctcggcg gcgattgtcg gggtgggagg
     3241 ggcgttcacg ttcctgtcgc cgctactcct cgacccgatc ttcaacaagt tcacgccgct
     3301 gcccgagggg ccgctgcgga ccgaggtgct gtcgctcgct gagaaggcgg gcgtggacgt
     3361 gggcgaggtc taccgggtcg acgcgagccg gcggaccacc ggggtgaacg cgtacgtgtg
     3421 gggcctcggg cgcacgaagc gagttgtgct ctacgacacg ctgattgacg gctttcccgc
     3481 ggaccaggtg cgatcggtcg tggcgcacga gctcagccac gtgaagcacc gcgacgtgcc
     3541 gcgcggcctg ctctggctcg cgatcgtcgc gccgttgggg acgctgttag tgaaggagat
     3601 cgcgaactcg ctcagcgacg actccgaacc ctcgcccgcg atgcttccgg ccctggcgct
     3661 cgcaacgtcg cttgtgtcgt tctggggcca ggtcgccggg aacgtgctct cgcgggcggt
     3721 ggagaggcgg gcggacgcgt tcgccttgtc gttgacggac gagcccgagc ccttcatcga
     3781 gatggagcgg gcgctgagcg tgaagaacct gggcgacccg agcccgccgc ggctgatgca
     3841 gctcttgttc ggcacccacc cggacacgct ggagcggatt gggatcggag tggcgtgggg
     3901 cacccgtggg ggcggcgccg cccccgcgga acagcagcag taaggggaag cggggcgagt
     3961 tcttcgacgc ggtagggggc gaattctccg aaccgtggtg ctttgtgccg catatggcgg
     4021 caaaatcgac cacggtagag cggaaactcg ctcgactagc ggcggaggcc cacggcgttg
     4081 tcacccggga ggagttactc cggctcggcg tgacgcgaca cgagatccag gatcgactgg
     4141 gcgcgggcgc gctgattcgg cagtacgccg gcgtgtatcg cgtcggccac tccgctccca
     4201 gcctcgaagc ccgctacatc gccgctgtaa aagcctgcgg taagggtgcc gtcctgagcg
     4261 ggcgcgccgc tgcctacctg ctccgcctga tcaagggaga tcttgcgccg ccggcggagg
     4321 taaccgcgcc tacgaagagg cgagtgaagg gagtcatcac gcatcgagga caaccgaaga
     4381 ccatgaccca ccggggcgtg ccgatcactt cagtgccctc cacgctcgtc gacctcgcgg
     4441 caacactcac ccccgatgag ctcgcacgtg cctgccacga agcgggggtt ctctacaaga
     4501 ccactccgaa gcaggtcgag gccgtcctcg cggaacgccc gaaagctaag ggcgccggca
     4561 atctccgcaa ggtcctccga ggcgagctgc gcgtcacgtg ctcgagcgcg agttcatcaa
     4621 ggcgctcaaa gcacacgggc ttccgttgcc gatcacaaac cgcgtcgccg gtgcccaccg
     4681 cgtcgactgc tactggccgg agcacaagct cgtcgtcgag ctcgactcct acacgtatca
     4741 ccagtcacgc cacgcctggg a
//
LOCUS       DASVXS010000184         7738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1015664, whole genome shotgun sequence.
ACCESSION   DASVXS010000184 DASVXS010000000
VERSION     DASVXS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015664"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VF752_10245"
     CDS             complement(<1..589)
                     /locus_tag="VF752_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855969.1"
                     /translation="MSALVPEQPTLTIYERSEEGRRAFTPPPLDVPETPISELLPEDA
                     IRRESPALPEISEPEIVRHYNNLSKKNFDLDTGFYPLGSCTMKHNPKVNERVAALPGH
                     ARLHPLQDPEYAQGALELMWNLQEALAEISGLPHVSLQPSAGSHGELTGLLLTRAYHE
                     ERGQERHKVLTPDTAHGTNPATVTMAGYEVVKVGTA"
     gene            complement(586..1926)
                     /gene="gcvPA"
                     /locus_tag="VF752_10250"
     CDS             complement(586..1926)
                     /gene="gcvPA"
                     /locus_tag="VF752_10250"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX8855970.1"
                     /translation="MTRYTSATDRDRREMLDAIGAESLDDLFSEIPEEVRLQRALELP
                     EGMSEQEVFEHLRSLAARNRHNDEEVSFLGAGMYDHYVPALIDSIISRSEFLTPYTPY
                     QPEISQGGLQVMFEFQTAISELTGLPVSNASLYEGPSSVASAGWLARQENRRTKFVAS
                     RGLHPHSRETLRTYSAGFGTEVVETPLDERGVTDLEALAAAIDDDTSAVFLQQPNFLG
                     TVEDLGALAEAAKRTSAVLVCQVDPMTLGVLRPPGDYGVDVAVGEGQPLGNRLDFGGP
                     SFGFFAATEKYIRRMPGRIAGETVDAEGRRGFVLTLQTREQHIRREKATHNICTSQAL
                     NALAGVIYLSWLGRRGIVEIGELMLRRTAYAREALGLELLNPGPVVREFAVRVPDVDG
                     VFERARAQGINPGYRLGRDYPEYEDGLLVAITERRSRADIDRLAGVLARQEVAA"
     gene            complement(1926..2315)
                     /gene="gcvH"
                     /locus_tag="VF752_10255"
     CDS             complement(1926..2315)
                     /gene="gcvH"
                     /locus_tag="VF752_10255"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX8855971.1"
                     /translation="MSEETYPDDLRYHQEHDWARVEGDTATFGITWYAQDQLGEVVFF
                     DPPDVGTEVSADASYAEVESVKAVSDVIAPLSGEIVEVNEALKETPERINEDPYGEGW
                     MVRVRLADPSQADGLMDAAAYRETIGK"
     gene            complement(2312..3394)
                     /gene="gcvT"
                     /locus_tag="VF752_10260"
     CDS             complement(2312..3394)
                     /gene="gcvT"
                     /locus_tag="VF752_10260"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX8855972.1"
                     /translation="MAPTTETLRRTPLFERHEAAGAKLVPFAGWEMPVQYEGIRQEHV
                     AVRTASGAFDVSHMGEIETSGPQAEAFLQRLLSNDVTKIAENGAQYALLCREDGGVLD
                     DLFTYRLGDRFLTVTNAANHERDFAWFQKQAEGFDVQVRDAASDYAMLALQGPTARDI
                     LRGLIEGEEPARMRTVRARVAGVECLVAGTGYTGEDGVELLIPPDGAAQVWDALVEAG
                     AKPTGLGARDTLRLEVCFHLYGNDLSEDRNPIEAGLGWACKLDTNFIGSDALRGFEPT
                     QRLVPFAFTGPGIPRQGNPVLVAGEPVGEVTSGSLSPCLDIGIGMGYVPVAAAEPGTK
                     IEIDVRGKLRPAEVREKPLYRKEEGS"
     gene            complement(3439..4530)
                     /gene="ftsZ"
                     /locus_tag="VF752_10265"
     CDS             complement(3439..4530)
                     /gene="ftsZ"
                     /locus_tag="VF752_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8855973.1"
                     /translation="METGSYLAVIKVVGVGGGGTNAVNRMVDAGLKGVEFIAVNTDAQ
                     ALQMCDADIKLQIGSQLTRGLGAGAEPEVGYGAANESRDEIKEALKGADMVFVTAGEG
                     GGTGTGAAPVIAEIAKHEIGALTVGVVTRPFEFEGSQRQRQATDGIQRLREQVDTLIV
                     IPNEKLLAIVERRTSILDAFREADNVLRQGVQGITDLITIPGLINLDFADVRTIMHDA
                     GSALMGIGAASGEQRASEAAKAAISSPLLEQSVEGATGLLLNITGSSDLGLFEVNEAA
                     EIVQSASDRDSNIIFGAVIDDALSDEVRVTVIATGFDKSGRPTVFDAERPSRQEPRRE
                     RGDISMDERQRSSLEISDDEIDIPSFLKD"
     gene            complement(4762..5724)
                     /locus_tag="VF752_10270"
     CDS             complement(4762..5724)
                     /locus_tag="VF752_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX8855974.1"
                     /translation="MRPLAQATARTLPRLPLIIPAVWRRRLVLAALVAAALVALYMAW
                     FRDSSFVQVENVTVTGLTTGDAAQVRAALVGEARDMSTLHVRRDRLERAVQGFAVVRS
                     LEVSTDFPHGLAIRVIERRPAALLKAAGAQAIVAPDGTLLRNVDVRGKLPVLRLSSLP
                     TARLTDGTPARALEVVAAAPPALAARLSSVRVEAGSGLVGRLRNGPKIVFGSSQRLGD
                     KWAAAASVLADPSSRGASYVDVRLPERPVAGGLAVQSVAPTDTAAPPASPATTQPAPQ
                     QQVTPTTPSAAAEPQTPTQGAIQPTQQTPATGTTGGAVPAPDTG"
     gene            complement(5721..6629)
                     /gene="murB"
                     /locus_tag="VF752_10275"
     CDS             complement(5721..6629)
                     /gene="murB"
                     /locus_tag="VF752_10275"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX8855975.1"
                     /translation="MSRPDELQPDYPLARLTTVRTGGQADWFARVVSAERLSALLAWA
                     EAEGVQVGVVGSGSNLLIADGGFRGLVLKLDDDLATIDREGDRVLAGGGARLPQASAR
                     AARWGLSGLEFGVNIPGTVGGAVKMNANAYGGELGKVLEWAEIATPEGSERRGPEQLG
                     FQYRRSALGPREIVARASFALEPADPEAVKATLAEMRAARKAAQPSGIKTFGSTFKNP
                     DDPRAEGRTAGQLLEAAGCRGLQVGGARFSEKHANFVENMGEATTADVIALMAEGRRR
                     VREQFGVVLEPEVQFLGDVDAQELWS"
     gene            complement(6626..>7738)
                     /locus_tag="VF752_10280"
     CDS             complement(6626..>7738)
                     /locus_tag="VF752_10280"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX8855976.1"
                     /translation="EDNPELAGARAAGAKVLHRGDLLAEAVALKRAIAVSGTHGKTTT
                     AGMIAHALVTRGRGAGYLIGGELRATGANAEWGEGEWIVVEADESDRSFLKLEPEVAV
                     VTNVELDHHTTYRSLHELERAFAQFAGGADRRILPASLELPGVEPSITFGVGEGDLAA
                     SDVELVALGSRFSVDRVAVELAVPGLHNVANAVAALAACREAGLPLDEAAQALASFEG
                     AGRRFEPHGATAQGAAVYDDYAHHPTEVRATLEAARSLGAERVVAVFQPHLYSRTRHL
                     AREFGRALALADVVLVLDVYPARERADDFPGVSGWLVAGAASDAARGRPVYWTPTLAD
                     AERLLAELLRPGDVLLTLGAGDVDRLARGLTERAAA"
BASE COUNT         1052 a         2803 c         2701 g         1182 t
ORIGIN      
        1 gcgccgtgcc caccttcacc acctcgtagc cggccatcgt cacggttgcg ggattggtgc
       61 cgtgcgcggt gtcgggcgtg agcaccttgt ggcgctcctg cccgcgctcc tcgtggtagg
      121 cgcgcgtgag cagcaggccg gtgagctcgc cgtgcgagcc ggccgaaggc tgtagcgaca
      181 cgtgcggcag cccggagatc tccgccagcg cctcctgcag gttccacatc agctcgagcg
      241 cgccctgcgc gtactcgggg tcctgcagcg ggtggagccg cgcgtggccc gggagcgccg
      301 ccacccgctc gttcaccttg gggttgtgct tcatcgtgca cgacccgagc gggtagaagc
      361 cggtgtcgag gtcgaagttc ttcttcgaga ggttgttgta gtggcggacg atctccggct
      421 cggagatctc gggcagcgcg ggcgactcgc gccggatcgc gtcttcgggc agcagctccg
      481 agatcggcgt ctcgggcacg tcgagcggcg gcggcgtgaa ggcgcggcgg ccctcctccg
      541 agcgctcgta gatcgtgagc gtgggctgct cgggcacgag cgcgctcatg cggccacctc
      601 ctgccgtgcc agaacgccgg cgaggcggtc gatgtcggcg cgcgagcgcc gctcggtgat
      661 cgcgacgagc aggccatcct cgtactcggg gtagtcgcgg ccgagccgat agccggggtt
      721 gatgccctgc gcccgggcgc gctcgaacac gccgtcgacg tccggcacgc gcacggcgaa
      781 ctcgcgcacc acgggccccg ggttcagtaa ctccaggccg agcgcctcgc gcgcatacgc
      841 ggtgcggcgc agcatcagct cgccgatctc gacgatcccc cgccggccga gccacgacag
      901 gtagatcacg cccgccagcg cgttcagcgc ctgcgaggtg cagatgttgt gcgtggcctt
      961 ctcgcggcgg atgtgctgct cgcgcgtctg cagcgtgagc acgaacccgc gacggccctc
     1021 ggcgtcaacg gtctcgcccg cgatccgccc cggcatgcgg cggatgtact tctcggtggc
     1081 ggcgaagaag ccgaacgacg ggccgccgaa gtcgagccgg ttgccgagcg gctggccctc
     1141 gcccaccgcc acgtccaccc cgtagtcgcc gggcggccgc aacacgccga gcgtcatcgg
     1201 gtcgacctgg cagaccagca cggcgctcgt gcgcttggcg gcctcggcca gcgcaccgag
     1261 gtcctccacc gtgccgagga agttcggctg ctgcaggaac accgccgagg tgtcgtcgtc
     1321 gatcgcggcc gcgagcgcct cgaggtccgt gactccgcgc tcgtcgagcg gcgtctccac
     1381 cacctcggtg ccgaaccccg ccgagtaggt gcggagcgtc tcgcgcgagt gcggatgcag
     1441 gccgcgcgag gcgacgaact tggtgcgccg gttctcctgc cgcgcgagcc agcccgctga
     1501 ggcgaccgag gagggcccct cgtacagcga cgcgttcgag acaggcaggc cggtgagctc
     1561 cgagatcgcg gtctggaact cgaacatcac ctgcaggccg ccctgcgaga tctcgggctg
     1621 gtagggcgtg tacggggtta ggaactccga gcgcgagatg atcgagtcga tcagcgccgg
     1681 cacgtagtgg tcgtacatgc ccgccccgag gaagctgacc tcctcgtcgt tgtggcggtt
     1741 cctggccgcg agcgaccgca ggtgctcgaa cacttcctgc tcgctcatgc cctccggcag
     1801 ctcgagcgcg cgctgcaggc gcacctcctc cgggatctcg gagaacaggt cgtcgagcga
     1861 ctcggcgccg atggcgtcga gcatctcccg gcggtcccgg tctgtggcgg aggtgtagcg
     1921 cgtcattact tcccgatcgt ctccctgtaa gcggcggcgt ccatcaggcc gtcggcctgc
     1981 gacgggtcgg cgaggcggac cctgaccatc cacccctcac catagggatc ctcgttgatg
     2041 cgttcgggcg tctccttgag agcctcattc acctcaacga tctctccgga cagcggggcg
     2101 atcacgtccg agaccgcctt cacggactcc acctcagcgt aggacgcgtc ggcggagacc
     2161 tcggttccca cgtcgggcgg gtcaaagaag acgacctcgc cgagctggtc ctgggcgtac
     2221 caggtgatgc cgaacgtggc cgtgtcgccc tcgacccgcg cccagtcgtg ctcctggtgg
     2281 tagcgcagat cgtcggggta ggtctcttcg ctcaactgcc ctcctccttg cgatagagcg
     2341 gcttctcgcg gacctccgcg ggccgcagct tgccgcggac gtcgatctcg atcttggtgc
     2401 ccggctccgc ggcggcgacc ggcacgtagc ccatcccgat cccgatgtcg aggcacggtg
     2461 acaggctgcc gctcgtgacc tcgccgacgg gctccccggc gaccagcacc gggttgcctt
     2521 gccgcgggat gcccggcccg gtgaacgcga acggcacgag ccgctgcgtg ggctcgaatc
     2581 cgcgcagcgc gtccgagccg atgaagttcg tgtcgagctt gcaggcccag cccagaccgg
     2641 cctcgatcgg gttgcggtct tccgacaggt cgttgccgta gaggtggaag cagacctcga
     2701 gcctcaatgt gtcgcgtgcg ccgagtcctg tgggcttcgc cccggcctcg acgagtgcgt
     2761 cccagacctg ggccgccccg tccggcggga tcagcagctc gacgccgtcc tcgccggtgt
     2821 agcccgtccc ggccacgagg cactcgacgc ctgccacgcg cgccctgacg gtgcgcatgc
     2881 gtgccggctc ctcgccctcg atcaggccgc gcaggatgtc gcgcgcggtc gggccctgga
     2941 gcgccagcat cgcgtagtcc gaggcggcgt cccgcacctg tacgtcgaag ccctcggcct
     3001 gcttctggaa ccaggcgaaa tcgcgctcgt ggttggccgc gttggtgacg gtcaagaacc
     3061 tgtcgccgag tcggtaggtg aagaggtcgt cgagcacgcc gccgtcctcg cggcagagca
     3121 gcgcgtactg ggcgccgttc tcggcgatct tcgtcacgtc gttcgacaag agccgctgca
     3181 ggaaggcctc ggcctgcggc ccgctcgtct cgatctcgcc catgtgcgag acgtcgaacg
     3241 cgccggacgc ggtgcggacc gccacgtgct cctggcggat gccctcgtac tgcaccggca
     3301 tctcccagcc cgcaaacggc acgagcttcg ctccggccgc ctcatggcgc tcgaaaagcg
     3361 gggtgcggcg gagtgtctcg gtggtcggcg ccagagcgcc gtgaccttac caacgccggg
     3421 ggaagccccg gcccggtatc agtccttcag gaaggacgga atgtcgatct cgtcgtccga
     3481 gatctcgagc gacgagcgct ggcgctcatc catcgagatg tcgccccgct cgcggcgcgg
     3541 ctcctggcgc gacgggcgct cggcgtcgaa cacggtcggg cgaccgctct tgtcgaagcc
     3601 ggtggcgatc acggtcacgc gcacctcgtc cgagagcgcg tcatcgatca cggcgccgaa
     3661 gatgatgttc gagtcgcggt cggaggcgct ctgcacgatc tccgcggcct cgttcacctc
     3721 gaacagcccg aggtcgctcg accccgtgat gttcagcagc aggccggtcg ctccctcaac
     3781 cgactgctcg agcagcgggc tcgagatcgc ggccttcgcg gcttccgacg cgcgctgctc
     3841 gccgctcgcc gcgccgatgc ccatcagcgc cgagccggcg tcgtgcatga tcgtccgcac
     3901 gtcggcgaag tcgaggttga tcaggcccgg gatcgtgatc aggtcggtga tgccctgcac
     3961 gccctggcgg agcacgttgt cggcctcgcg gaacgcgtcg aggatgctcg tgcggcgctc
     4021 cacgatcgcg agcagcttct cgttcgggat cacgatcagc gtgtccacct gctcgcgcag
     4081 ccgctggatg ccgtcggtcg cctggcgctg gcgctgcgag ccctcgaact cgaacgggcg
     4141 ggtgacgacg ccgacggtga gcgcgccgat ctcgtgcttg gcgatctccg cgatcacggg
     4201 ggccgccccg gtgccggtgc cgccgccctc gccggcggtg acgaacacca tgtcagcgcc
     4261 cttcagcgcc tccttgatct cgtcgcggct ctcgtttgcc gcgccgtatc cgacctccgg
     4321 ctccgcgccg gctccgaggc cgcgcgtgag ctggctgccg atttgcagct tgatgtcggc
     4381 gtcacacatc tgcagcgcct gcgcgtcggt gttgacggcg atgaactcga cgcccttcag
     4441 gccggcgtcg accatgcggt tcaccgcatt ggtcccgcca ccgccgactc cgacgacctt
     4501 gatgaccgcc aggtagctac ccgtctccat cgtcgtgttc ctcgcctcgc ctaacccgtt
     4561 tctttagcgc tgggcgcaga tttttccgtc cgagtctcta ttgacacttg agggtttcgg
     4621 acccgccccg ccagccgcaa agtccgcact gtacgcgttt tttcggagaa gtgtcaacgg
     4681 ttggcacgat caattgcagg gtctaaccct ctaccgagag ttgaggcctg tgcgaactct
     4741 cgactggtgg ttgagactag ctcaccccgt gtcgggcgcc ggcacggctc cgccggtcgt
     4801 gccggtcgcg ggtgtctgct gtgtgggctg gatcgcgccc tgcgtaggcg tctgaggctc
     4861 ggccgcggca gacggcgtcg tgggcgtcac ctgctgctga ggagctggct gcgtcgtcgc
     4921 cggcgacgcc ggtggcgcgg cggtgtctgt cggcgccacg gactgcacgg caagaccgcc
     4981 tgcaacgggt cgctcgggca ggcggacgtc gacgtaggaa gcgcccctcg atgaggggtc
     5041 tgcgagcacg ctcgccgccg ccgcccactt gtcgccgagg cgctgcgagg agccgaacac
     5101 gatcttcggg ccgttgcgga gccggcccac gagccccgac ccggcctcga cgcggacgct
     5161 cgacaaccgt gccgctagcg ccggcggcgc ggccgccacg acctcgagcg cccgtgccgg
     5221 cgtgccgtcc gtgaggcggg ctgtcgggag cgagctcagc cggagcacgg ggagcttgcc
     5281 gcgtacgtcc acgttgcgaa ggagggtgcc gtcgggcgcc acgatcgcct gcgcaccagc
     5341 ggctttcagc agcgctgcgg ggcgccgctc gatcacgcgg atcgcaagcc cgtgcgggaa
     5401 gtcggtcgag acctcgagcg agcggacgac cgcgaagccc tgaaccgcac gctcgagccg
     5461 gtcgcgccgc acgtggagag tgctcatgtc gcgagcctca cccacgagcg cggcgcgcac
     5521 ctgggccgcg tcgcccgtgg tgagcccggt cacggtcacg ttctcgacct gcacgaacga
     5581 gctgtcgcgg aaccaggcca tgtacagggc gaccagcgcg gccgcgacta gcgctgcgag
     5641 cacgaggcgc ctacgccaga cggcgggaat gatgagcggg aggcgcggca gcgtgcgcgc
     5701 cgtagcctgc gcgagcggcc tcacgaccag agctcctgcg cgtccacgtc gccgaggaac
     5761 tgcacctcgg gctcgagcac cacgccgaac tgctcgcgca cgcgccgcct gccctccgcc
     5821 atcagcgcga tcacgtcggc ggtcgtggcc tcgcccatgt tctcgacgaa gttcgcgtgc
     5881 ttctcggaga agcgggcgcc gcccacctgc aggccgcgac agccggccgc ctcgagcagc
     5941 tgtcccgcgg tccggccctc ggcgcgcggg tcgtcgggat tcttgaacgt ggagccgaag
     6001 gtcttgatgc ccgacggctg cgccgctttg cgcgccgcgc gcatctcggc aagcgtggcc
     6061 ttcacagcct cggggtcggc gggctcgagc gcgaacgagg cgcgcgccac gatctcccgg
     6121 gggccgagcg ccgagcgccg gtactggaag ccgagctgct cgggaccgcg ccgttcgctg
     6181 ccttccgggg tggcgatctc cgcccactcg agcactttgc cgagctcgcc gccgtacgcg
     6241 ttcgcgttca tcttcaccgc tccgccgacc gtgccgggga tgttgacgcc gaactcgagg
     6301 ccggagagac cccagcgggc cgctcgcgcg gatgcctgtg gaaggcgggc gccgccgccg
     6361 gcgagaacgc ggtcgccctc gcggtcgatc gtcgcgaggt cgtcgtccag cttcagcaca
     6421 agtcctctga atccgccatc cgctatcagc aggttcgatc cggagccgac cactcctacc
     6481 tgcacgccct ccgcctcggc ccacgcgagc agggcggaaa gccgctccgc ggacaccacg
     6541 cgcgcgaacc agtccgcctg cccgccggtg cgcacggtgg tgagccgggc caacggatag
     6601 tccggctgga gctcgtccgg gcgactcacg cggccgcccg ctcagtaagg ccccgcgcga
     6661 ggcggtccac gtcgccagcc ccgagcgtca gcagaacgtc gcctggccgc agcagctcgg
     6721 ccaagaggcg ctcggcgtcg gcgagcgtcg gcgtccagta cacgggccgg ccgcgagcgg
     6781 cgtccgaagc cgcccccgcc acgagccagc cggatacgcc ggggaagtcg tccgcccgct
     6841 cacgcgccgg gtagacgtcg agcaccagca ccacgtcggc gagcgccaaa gcgcggccga
     6901 actcgcgcgc caagtgtctc gtgcgcgagt agaggtgcgg ctggaagacg gccaccaccc
     6961 gttcggcgcc taggctccgc gcggcctcga gcgtggcgcg gacctcggtc gggtggtggg
     7021 catagtcgtc gtacacggcc gcgccctgcg ccgtcgcgcc gtgcggctcg aagcggcgtc
     7081 ccgcgccctc gaagctcgcg agcgcctgcg cggcctcgtc gagcggcaga cccgcctcgc
     7141 gacaggcggc cagcgccgcc actgcgttcg ccacgttgtg caggccgggc accgcaagct
     7201 ccaccgccac ccggtcgacg ctgaagcgcg agccgagggc cacgagctcg acgtcgctcg
     7261 cggccaagtc gccctcgccg accccgaacg tgatgctcgg ctcgactccc ggtagctcga
     7321 gtgacgccgg gaggatgcgc cggtcggccc cgcccgcgaa ctgagcgaag gcacgctcga
     7381 gctcgtggag ggagcggtag gtggtgtggt ggtcgagctc gacgttggtg acgacggcaa
     7441 cctcgggctc cagcttgagg aaggagcggt cggactcgtc cgcctcgacc acgatccact
     7501 ccccctcgcc ccactccgcg ttggcgcccg tggcccgcag ctcgccgccg atcaggtagc
     7561 cggcgccgcg gccgcgcgtg acgagcgcgt gggcgatcat cccggccgta gtcgtcttgc
     7621 cgtgcgtgcc ggacacggcg atcgcgcgct tcagcgcgac cgcctccgca agcaggtcgc
     7681 cgcggtggag caccttcgcc ccggccgcgc gcgcgccggc aagctccggg ttgtcctc
//
LOCUS       DASVXS010000185         4779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_731718, whole genome shotgun sequence.
ACCESSION   DASVXS010000185 DASVXS010000000
VERSION     DASVXS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731718"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="VF752_10285"
     CDS             <1..1979
                     /locus_tag="VF752_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8855977.1"
                     /translation="AGGNPLFAEEMVRLLVDGGTVSGSELPQTVQALLAARLDSLDPL
                     ERRIVQHAAVVGRTFWEGALIRVAAEEGAELGQALAALQSKDIFLPGEAGTLAGEPEL
                     AFKHVLIRDVAYGMLPKAVRARKHFEVGDFIETRAGDRTDEVVALLAEHYGRAATLGR
                     EARVDIAELDEMRSRALRFLEAAGDAAASFFSNREAFAHYEAGRELGGDDEEAMARIG
                     EKQGDAALRLGRIDSAISVWGESLDYHRRQENLERVADLHRKIGAAFWHAGDRKQAIE
                     HHQKGINLLKDGPPCMELVRLYEEAAWLYMQTGDNMLAIYASEKALRLAERLGETRAA
                     SRAHGIFGRVFGRIGDTERARENLERSVELARGSDEGETILALLALGQHLEVSEADYS
                     GADAAYQEALSLAEQVGDVPAQVELHAALAWLAVYRADWDSVRASGEASAELSEREGL
                     AGKLSLPFLLRGLLAWRQGDFDEAEVQFTRSHELAEQAGWSEVAFSALFALATDLRDR
                     GDYSSAVTALAQALDVCERAGLIAQSIQAISQRAVVLGLAGKGAQAREAAIESTELAE
                     RLHYPVARAAALEADGFSADPGEGIDRLEQARVAWQELGRPLDAARCSMLVGRALLDE
                     DAVRATAALDRAADEFAALGVRHLAEGARALVQG"
     gene            2046..2216
                     /locus_tag="VF752_10290"
     CDS             2046..2216
                     /locus_tag="VF752_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX8855978.1"
                     /translation="MRVIECDCGATLTAANDDELFDTVRNHVDEAHPDQDFSDGQIRQ
                     TIASQAYDATDS"
     gene            complement(2274..3275)
                     /locus_tag="VF752_10295"
     CDS             complement(2274..3275)
                     /locus_tag="VF752_10295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX8855979.1"
                     /translation="MTVGSPLQGGGRRRRAKGPLVLVLLVALLAGGATYAYTQGHLDL
                     FSSSKSKAHGAATGSVNAGQPGEHPPPVPGSPYAVALEAADVFRLHFHQPPRAGLVFD
                     LRSGQVLWRLHPYRRLPIASLTKIMTAVVVTGRTTPREKARVTKAALNYSGSGVGLLP
                     KGRHVTVWALMNGLLLPSGNDAALALADHVGGSERRFVRLMNGRARKMGLRCTHYVSP
                     HGLEPGNRSCAADLAAMARVAMRRPEIERIVRQRQNVVRFPIKGGRLYLNTTNPLIRS
                     GYVGTIGLKTGDTDEAGHCFVGVVRRGGRTLGVVLLHSPNVLEQSKKLLSKGFRTHR"
     gene            complement(3262..3444)
                     /locus_tag="VF752_10300"
     CDS             complement(3262..3444)
                     /locus_tag="VF752_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855980.1"
                     /translation="MGRDAALLGAVIVIGLLAVGMVRWVTDNGVNALVIISGVILVVL
                     AIGVLGALGTPPNDRR"
     gene            complement(3444..4274)
                     /locus_tag="VF752_10305"
     CDS             complement(3444..4274)
                     /locus_tag="VF752_10305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8855981.1"
                     /translation="MIPLHDNIPTRRTPILTIAIIAINVVVFLFLQHPQGFGSVDNKI
                     VVEWGTIPYELTHPGKECALFGGQNGQVICAGETATDSTTGQTVRVTERDVERQDPNQ
                     PPAWVTIFSSMFMHGGVLHIAGNMLFLWIFGNNIEDSMSRWRFVLFYLLGGVAAVAGQ
                     TAIDPNATVPSLGASGAIAAVLGGYALLYPRARVLTIILIIFFFTFIELPALVVLGLW
                     FLIQVFYGASQIGAPVGSGGGVAYFAHIGGFVFGLLLIKLFANRVNPDYEGGSHLPVY
                     "
     gene            complement(4327..>4779)
                     /locus_tag="VF752_10310"
     CDS             complement(4327..>4779)
                     /locus_tag="VF752_10310"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX8855982.1"
                     /translation="FRGEEMPLARVIAERPDVFNHNVETVPRLYPLARRGSKFLRSCR
                     VLANAKQMGGDEVVTKSGLMTGLGESKEELIETLGILREHDVQVLTVGQYLRPTKNHL
                     PVVRYWHPDEFAELEAAAYAMGFESVAAGPLVRSSYHAEQTLEQARSV"
BASE COUNT          747 a         1616 c         1697 g          719 t
ORIGIN      
        1 gggcgggcgg caatccgctc tttgccgagg agatggtgcg cctgctcgtg gacggcggca
       61 ccgtgtcggg cagcgagctg ccgcagaccg tgcaggcgct gctagccgcg cgcctcgact
      121 ccctggatcc gcttgagcgc cggatcgttc agcacgcggc ggtggtgggc cgcaccttct
      181 gggaaggagc gctaattcgg gtcgcggcgg aggagggcgc cgagctcggg caggcgctcg
      241 ccgcacttca gtcgaaggac atcttcctgc cgggcgaggc cgggacactc gcgggcgagc
      301 ccgagctcgc cttcaagcac gtgctcattc gcgacgtggc gtatggcatg ttgccgaagg
      361 cggtgcgggc gcggaagcac ttcgaggtcg gcgacttcat cgagacccgc gcaggcgacc
      421 gcaccgacga ggtggtggcg ctcctcgcgg agcactacgg ccgagccgcg acgctcggcc
      481 gcgaggcacg cgtggacatc gccgagctcg acgagatgcg ctcgcgcgcg ctccgcttcc
      541 tcgaggccgc aggcgacgcc gccgccagct tcttctcgaa ccgcgaggcc ttcgcccact
      601 acgaggccgg tcgcgagctc ggcggcgacg acgaggaggc gatggcgcgc atcggcgaga
      661 agcagggcga cgccgcgctg cggctcggtc gcatcgacag cgccatctcg gtctggggcg
      721 agagcctcga ctaccaccgg cggcaggaga acctggaacg cgtggccgac ctgcaccgca
      781 agatcggcgc tgccttttgg cacgccggcg accgcaagca ggcgatcgag caccaccaga
      841 agggcatcaa cctgctcaag gacgggccgc cctgcatgga gctcgtgcgc ctgtacgagg
      901 aggccgcgtg gctctacatg cagacgggcg acaacatgct cgcgatctac gcctcggaga
      961 aggcgctccg cctggccgag cgtctcggcg agacgcgcgc ggcgagccgc gcccacggca
     1021 tcttcgggcg cgtgttcggc cgcatcggcg acaccgagcg cgcccgcgag aacctcgagc
     1081 gttcggttga gctggcgcgc gggtccgacg agggcgagac gatcctggcg ctgctcgccc
     1141 tgggtcagca cctcgaggta tcggaagccg actactccgg tgccgacgcc gcctatcagg
     1201 aggcgctgtc gctggcggag caggtgggcg acgttccggc gcaggtcgag ttgcacgccg
     1261 cgctcgcgtg gctcgcggtc taccgcgctg actgggactc ggtgcgcgcc tccggcgagg
     1321 ccagcgcgga gctgtctgag cgcgaggggc tggccgggaa gttgtcgctg ccgttcctac
     1381 tgcgcggcct gctggcctgg cgccagggcg acttcgacga ggccgaggtg cagttcaccc
     1441 gctcgcatga gctcgccgag caggcgggct ggtccgaggt cgcgttctcc gcgctgttcg
     1501 cgctcgccac ggaccttcgc gatcgcggcg actacagctc ggctgtgacc gcgctggcgc
     1561 aggcgctcga cgtgtgcgaa cgggccggcc tgattgcgca gtcgatccag gcgatctcgc
     1621 agcgtgccgt cgtgctcggt ctcgcgggca agggtgcgca ggcgcgcgag gcggccatcg
     1681 agagcacgga gctggccgag cggctgcact acccggtcgc gcgcgcggcc gcgctcgagg
     1741 cggacggctt cagcgccgac cccggcgagg gcatcgaccg tctcgagcag gctcgcgtgg
     1801 cctggcagga gctcggccgg ccactcgatg ccgcgcgctg ctcgatgctg gtcggccgcg
     1861 cgctgctcga cgaggacgcg gtgcgagcca ccgcggcgct tgatcgcgcg gctgacgagt
     1921 tcgccgcgct cggcgtgcgg cacctcgccg agggcgcacg cgcgctcgta cagggctgaa
     1981 cctgcgcgtg gacccggcct agccgggtat ctgcttcgct tgaacaggcg aaccaggaga
     2041 gacacgtgcg ggttatcgaa tgcgactgcg gcgccaccct gacggcggca aatgacgacg
     2101 agctcttcga cacggtgcgc aaccacgtgg acgaggcgca tccggaccag gacttcagcg
     2161 atggacagat ccgccagacg atcgcgtccc aggcgtacga cgccaccgat agctgatcgc
     2221 gccggggacg gatgagcgcg ccgccggtgc tcgtagagct gcctctcggc cggctagcgg
     2281 tgcgtgcgga accccttcga cagcagcttc ttcgactgct cgagcacgtt gggcgagtgg
     2341 agcagcacca cgccgagcgt cctcccaccg cggcggacca cgcctacgaa gcagtggccc
     2401 gcctcatcgg tgtcgccggt cttgagaccg atcgtcccca cgtagcccga gcggatcagc
     2461 ggattcgtcg tgttcaggta gagcctgccg cccttgatcg ggaagcgcac cacgttctgg
     2521 cgctggcgca cgatgcgctc gatctccggc ctgcgcatgg cgactcgcgc cattgcggcg
     2581 aggtcggcgg cgcacgagcg gttgccgggc tcgagaccgt gcggcgacac gtagtgcgtg
     2641 cagcgcagcc ccatctttcg ggcccgtcca ttcatcagcc tcacgaacct gcgctcgctg
     2701 ccgcccacgt ggtcggcgag cgcaagggcg gcgtcgtttc cggacggcag aagcaggccg
     2761 ttcatcagcg cccagaccgt cacatgccgc cccttgggca gaagtccgac gccggagccg
     2821 ctgtagttga gcgccgcttt cgtcacgcgc gccttctcac gcggggttgt gcggcccgtg
     2881 accacgaccg cggtcatgat cttcgtgagg ctggcgatgg gcagccgcct gtacgggtgc
     2941 agccgccaca agacctgtcc ggatcgcaga tcgaacacga ggccggcgcg cgggggctgg
     3001 tggaagtgga ggcggaacac gtccgcagct tcgagcgcca cggcgtacgg cgagccgggc
     3061 accggcggcg ggtgctcgcc cggctgcccc gcgttcacac ttccggtcgc ggcgccgtgc
     3121 gctttcgact tcgagctgct gaacaggtcg agatggccct gcgtatacgc gtaggtggca
     3181 ccgccggcca gcagcgcgac cagcaacacg agcacgagcg ggcccttcgc gcggcgccgg
     3241 cggccgcctc cttggagcgg gctaccgacg gtcattgggt ggggtgccga gcgcgccgag
     3301 cacgccgatc gcgaggacta ccagaatcac gccggagatg atgacgagcg cgttgacacc
     3361 gttgtccgtc acccacctga ccatgccgac cgcaagcagg ccgatcacga tgaccgcgcc
     3421 gaggagcgct gcgtctctcc ccatcagtag acgggcaagt gggacccgcc ctcgtagtcg
     3481 gggttcaccc ggttggcgaa cagcttgatc agcagcaggc cgaacacgaa tccgcctatg
     3541 tgagcgaagt aggcgacgcc gccgcccgag ccgacgggtg cgccgatctg ggatgcgccg
     3601 tagaacacct ggatgaggaa ccagaggccg agcacgacca gcgccggcag ctcgatgaag
     3661 gtgaagaaga agatgatcag gatgatcgtc aggaccctgg ctctcggata cagcagcgcg
     3721 tagccgccga gcaccgccgc gatggcgccg ctcgccccca agctcggaac ggttgcgttc
     3781 gggtcgatcg ccgtctgacc ggcgacggcc gccacgcccc cgagcagata gaagagcacg
     3841 aatctccatc tgctcatcga gtcctcgatg ttgttgccga agatccagag gaacagcatg
     3901 ttccccgcga tgtggagcac gccaccgtgc atgaacatcg agctgaagat ggtcacccag
     3961 gccggcggct gattcgggtc ctgccgctcc acatctcgct cggtgactct gacggtctgc
     4021 ccggtcgtgc tgtcggtcgc ggtttcgccg gcgcagatca cctgaccgtt ctggccgccg
     4081 aacaacgcgc actctttccc cgggtgcgtg agctcgtatg ggatcgtgcc ccactcgacc
     4141 acgatcttgt tgtccacgct gccgaagccc tgcggatgct gcaggaacag gaagacgacg
     4201 acgttgatcg cgatgatcgc gatcgtgagg atcggcgtgc gacgcgtcgg gatgttgtcg
     4261 tgcagcggga tcacgaccgg cggagagttt catactcccc tgctcggcgg agcgacaatc
     4321 gcgtaactag accgagcgcg cctgctcgag agtctgctcg gcgtggtagc tcgaacgcac
     4381 gagcgggccg gccgcgactg actcgaagcc catcgcgtag gcggccgctt cgagctccgc
     4441 gaattcgtcc gggtgccagt agcgcaccac cggcaggtgg ttcttcgtgg gccgcaggta
     4501 ctggccgacg gtgagcacct ggacgtcgtg ctcgcgcagg atgccgagcg tctcgatcag
     4561 ctcttccttg ctttccccga ggccagtcat caggcccgac ttcgtgacga cctcatcgcc
     4621 gcccatctgc ttggcgttgg cgagcacgcg gcaggagcgc aggaacttcg agccgcggcg
     4681 agctagcggg tagagccgcg gaacggtttc gacgttgtgg ttgaacacgt cagggcgctc
     4741 ggcgatcacg cgggcgagcg gcatctcctc gccgcggaa
//
LOCUS       DASVXS010000186         8557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1394936, whole genome shotgun sequence.
ACCESSION   DASVXS010000186 DASVXS010000000
VERSION     DASVXS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394936"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VF752_10315"
     CDS             <1..165
                     /locus_tag="VF752_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX8855983.1"
                     /translation="VRSYNCLKRAGIQTVGELLQKSESELAAIPNFGRKSIEEVVETL
                     EARGLSLRQG"
     gene            189..554
                     /gene="rplQ"
                     /locus_tag="VF752_10320"
     CDS             189..554
                     /gene="rplQ"
                     /locus_tag="VF752_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010285110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX8855984.1"
                     /translation="MRHQRTRNKLSRSSAHRKALMRNLAREVIEHERIKTTEAKAKAV
                     KPEVEKLITLARRGDLHARRQAASWLGQSDAEVKGIVYKLFEEIAPRYADRPGGYTRI
                     LKLGPRRSDSTEMVFLELV"
     gene            571..1371
                     /locus_tag="VF752_10325"
     CDS             571..1371
                     /locus_tag="VF752_10325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8855985.1"
                     /translation="MDVEKLKEGTRKGWSQGDYSLISERLRPVAHVLVDECAISAGQE
                     VLDAAAGDGNVAIAAAEEGASVTACDITPMMIERGRERSEAEGVDIEWVEGDVEALPF
                     ADESFECVTSAFGLFLAPRPELAAREVFRVLRPGGTFGMTAWTREGFMGQVFEMNSRF
                     DPGPPGLPATMDWGNEAIVQSRLEGLAGSVRTERRTLRWEFDAPESYWNFLGKSGPTA
                     ARRAALEAGQLEQMRAALFEIIDRFNQADDGSVSVEGEYLLTVARKRG"
     gene            1392..2093
                     /gene="truA"
                     /locus_tag="VF752_10330"
     CDS             1392..2093
                     /gene="truA"
                     /locus_tag="VF752_10330"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX8855986.1"
                     /translation="MEYDGSGFSGWAAQPGLRTVQGELETALAALLHREVQVTVAGRT
                     DTGVHARGQVASHEGEPATVRGLNALLPDDIGVLESASIHDGFDARRDAKARTYRYRV
                     LARTAPSPFERRRALHWPHAIDHEALYACAAAVTGTHDFTAFTPTQTDHVRFERDVMR
                     AEWLEELDGVLAFWIEADTFMRHMVRTLVGSMLWVARGRFDVAWFEGLLHGRPRSEAG
                     DTAPPYGLYLESVRY"
     gene            2122..2934
                     /gene="surE"
                     /locus_tag="VF752_10335"
     CDS             2122..2934
                     /gene="surE"
                     /locus_tag="VF752_10335"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX8855987.1"
                     /translation="MKVLLTNDDGIGAKGLHALRRALLEVPGIELSVIAPDSNRSATA
                     RSITTRRPLQVEEVELGDGTTGYATDGTPVDCVRFAALGLVDQPDLIVSGINHGSNLG
                     DDITYSGTVAAALEGVVLGIPAIAVSQQSDRRQLDFHDGGGFDFTQVAAFAARIVEEL
                     EDVPIPADTLLNINAPAGEITGARACRLGKRIYHDRLELESEDDEGRRLYRIYGREPG
                     YEEDEGTDFAAIADGSIAVTPLHFDLTDEAGIEQLSGFELERLLRPAAREVE"
     gene            2927..4945
                     /gene="ligA"
                     /locus_tag="VF752_10340"
     CDS             2927..4945
                     /gene="ligA"
                     /locus_tag="VF752_10340"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX8855988.1"
                     /translation="MSSPAERAAELRREIEFHNHRYYVLDDPVVSDAEYDGLLNELRE
                     IEHEHPELLTPDSPTQRVGAPPLDKFEHVRHLQPMLSLANARGEDELRAWEKRVRNLL
                     ERQGLDDGKLEYVSEPKIDGLAISLVYENGVLARGATRGDGEIGEDVTQNLRTIKAIP
                     LRVEDAPRLFEVRGEIYLPRSAFARLNEQRAEAGEPTFANPRNSAAGSIRQLDQSIAA
                     SRPLSIWCYGIGAVEGIEFKTHKQSLDWLHDRGFKVNPDVQVHADVESLAARCREWEE
                     IRESLDYEIDGVVAKVNDLELQRALGVVGREPRGAIAWKFPPMTATTTLNDIGWNVGR
                     TGHMVPFAILEPVQVSGVTVKLATLHNEEDLLRKDIRPGDEVVVMRAGDVIPQVVSPS
                     PAAQRNPNRKPRESALPPEKCPACGTPTIKPEGGVWTICPNRENCPGQVLQAVKHFVG
                     AMDIDGFGEETAIRFLRDGVIDGIAGIYELDAEKLTALEGFGEISARNLLANIEASKQ
                     QPFNRVLYGLGIPGIGWVNARALASRFRSIDALMEADAEQIQETDGIGPVLAGTIEGT
                     LKEEHTRDLVRRLREHGLTMEEKGEAAAADGPLAGKTIVLTGTLPELSRERATELIEQ
                     AGGKVTGSVSKKTDYVVAGADAGSKLAKAQELGTDVLDEEGLRALLQA"
     gene            4993..5184
                     /locus_tag="VF752_10345"
     CDS             4993..5184
                     /locus_tag="VF752_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855989.1"
                     /translation="MGERHPERPFYLAAAFFLVLDLLLIASSSGQSWRVLLPVFALVL
                     LFLGVRRRREQRRDGHRRY"
     gene            complement(5181..6413)
                     /gene="dacB"
                     /locus_tag="VF752_10350"
     CDS             complement(5181..6413)
                     /gene="dacB"
                     /locus_tag="VF752_10350"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX8855990.1"
                     /translation="MRRLCLLAALAALLLLPAAAAAQSQDSKLRRVLASAMRHAGRSS
                     GAYVLDVTSRRVLFRASQDSARILASNTKLFTTSATLASYGSAATLATTVKGVGQAQP
                     DGTWPGDLYLVGGGDPTFGSDAFAKRNYGGGATVEVLADELEAAGITSVRGRILGDES
                     RFDSLRGGPDSHFGVSSYVGPLSALSYNRGLATGGFQSNPPAYAALQLEKALESRGIS
                     VRGQAQAGMAPPDAAPIATEQSPPMSRLVRLTIKPSDNFFAETLLKDLAALKGAPGTT
                     AAGARAARSFATRLGLRARIVDGSGLARGNQASPRMVVGLLDRVRARPEFQDLFDALP
                     VAGVDGTLHDRMRSGAAKSRCRAKTGTLSNVSALSGFCQSRGGDLIAFSFLMNRVRVT
                     GARSLQDRMANALAAYNG"
     gene            6501..7772
                     /locus_tag="VF752_10355"
     CDS             6501..7772
                     /locus_tag="VF752_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8855991.1"
                     /translation="MGNVVKLEKPARGRAARGGQTARRRRRRSKTALVLGGGGFTGGV
                     YEIGALRALDLLAVNRTVNEFDVYVGTSAGSFVASLAANGVTPEEMMRVVNQQVPTPF
                     HDIDLSRLLRPNYVDFAKSAALLPLRMVGIIRTMAANLNSVSLMDLVVGLAEGLPAGI
                     YNGSGIEGYLTDVLGDLDRTTDFRELKNQLFLTATDLDTCERIIFGEDGWDDISIPKA
                     AAASTALPMVYKPVELKGRQLVDGGIGSTTNLDIAVEKGAKFVVVVNPLVPYVNDFQK
                     LIPTMLGSRVRRVSDIGFPQIGYQAFKMLAHQRLHEAVRQWEERYPGVDIILIEPDPD
                     DELMFQTNILNFAKRVDIARHGFESVTFKLAKDYERFRSICAKHGIEISATRVRKVMR
                     QLERAEAEETRAWRRILEQTTGTLLRQSEDA"
     gene            complement(7887..>8557)
                     /locus_tag="VF752_10360"
     CDS             complement(7887..>8557)
                     /locus_tag="VF752_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8855992.1"
                     /translation="VDALIAACGDRTRVVVLCNPNDPTGTYLPAAEVARLAAALPGDA
                     HLLVDEAYVQFQDVEDEDAVLRLADEHPRVLVFRTFSKVYGLSGLRAGYAVGSTGTAR
                     LLDAIAPVLGVNALTQAVMSQALRIGDDEVRRRRMLVLVQRKRLLDALHDMPVDAPDS
                     QANFVWLRAPGLTGSELAARLERAGVLVAPGGPLGDDHHVRAAIRGAAATERLLEALA
                     GVAR"
BASE COUNT         1372 a         2953 c         3034 g         1198 t
ORIGIN      
        1 gtccgctcgt acaactgcct gaagcgtgcc ggcatccaga cggtcggcga gctgctgcag
       61 aagtccgagt ccgagctcgc cgcgatcccg aacttcggtc gcaagtcgat cgaggaggtc
      121 gtcgagaccc tcgaggcccg cggtctgagc ctgcgccagg gctagccgct ctctagactc
      181 cggacgccat gcgccaccag aggacacgca acaagctcag tcgaagctca gcgcaccgca
      241 aggcgctgat gcgcaacctc gcgcgcgagg tgatcgagca cgagcggatc aagacgaccg
      301 aggcgaaggc caaggccgtc aagcccgagg tggagaagct gatcacgctt gcccggcgcg
      361 gcgacctcca cgcccgccgg caggcggcgt cctggctcgg ccagtccgac gccgaggtga
      421 agggcatcgt ctacaagctg ttcgaggaga tcgctccgcg ctacgcggac cgaccgggcg
      481 gctacacgcg catcctcaag ctgggaccgc gccggtcgga ctcgaccgag atggtcttcc
      541 tcgagctggt ctaggcgacg ggcgtccagc gtggacgtcg agaagctcaa ggaggggacc
      601 cgcaagggct ggtcgcaggg cgactactcc ctcatctccg agcgtctgcg gccggtggcg
      661 cacgtgctcg tcgacgagtg cgcgatctca gcgggccagg aggtgctcga cgccgcggcc
      721 ggcgacggca acgtggcgat cgccgccgcc gaggagggcg cgtcggtgac cgcctgcgac
      781 atcacgccga tgatgatcga gcggggccgc gagcgcagcg aggccgaggg cgtcgacatc
      841 gagtgggtcg agggcgacgt ggaggcgctg cctttcgccg acgagtcctt cgaatgcgtc
      901 acctcggcat tcgggctgtt cctcgcgcca cgaccggagc tagcggcccg cgaggtgttc
      961 cgcgtgttgc ggccgggcgg cacgttcggc atgaccgcct ggacgcgcga gggcttcatg
     1021 ggccaggtgt tcgagatgaa cagccgcttc gaccccggac cgccgggcct accggccacg
     1081 atggattggg gcaacgaggc gatcgtccag tcgcggctcg agggcctcgc cggctcggtc
     1141 aggaccgagc ggcgcactct ccgctgggag ttcgacgccc ccgagtccta ctggaacttc
     1201 ctcggcaagt cggggcccac cgcagcgcgg cgcgccgcgc tcgaagccgg ccagctcgag
     1261 cagatgcggg ctgcgctatt cgaaatcatc gaccgcttca accaggcgga cgacggcagc
     1321 gtgtcggtcg agggcgagta cctgctcacg gtggcgcgca agcgcggctg accctgacct
     1381 cgcgcctgct gatcgagtac gacggctccg gcttctcggg ctgggccgcc cagcccggct
     1441 tgcggaccgt ccagggagag ctcgagaccg cgctcgccgc gctgctccac cgtgaggtcc
     1501 aagtcacggt ggcgggccgc accgacaccg gcgtccacgc gcgcggccag gtggcgagcc
     1561 acgagggcga gcctgcgacc gtgcggggcc tgaacgcgct gctgcccgac gacataggcg
     1621 tgctcgagag cgcgtcgatt cacgatgggt tcgacgcaag gcgtgacgcc aaggcgcgca
     1681 cataccgcta ccgcgtgctg gcgcgcaccg cgccgagccc cttcgagcgc cgccgcgctc
     1741 ttcactggcc gcacgcaatc gaccatgagg cgctctacgc atgtgccgcc gccgtcaccg
     1801 gcacccacga cttcaccgcc ttcacgccca cccagacaga ccacgtgcgc ttcgagcgcg
     1861 acgtgatgcg ggcggagtgg ctcgaggaac tggacggcgt cctggccttc tggatagagg
     1921 ccgacacgtt catgcgccac atggtgcgga cgctcgtcgg gtcgatgctc tgggtggcgc
     1981 gtggccgctt cgatgtggcg tggttcgagg gcctgctcca tgggcggccg cgctcggagg
     2041 cgggcgacac agcgccgccg tacgggctct atctcgagtc ggtccgctac tgaggcgtct
     2101 gactattctc agatgccggg catgaaagtc ctcctcacaa acgacgacgg catcggcgcg
     2161 aagggcctgc acgctctgcg ccgggccctg ctcgaggtgc ccggcatcga gctttccgtg
     2221 atcgcgccgg actcgaaccg gagcgcgacc gcgcgaagca tcaccacccg acgtccgctc
     2281 caggtcgagg aggtggagct cggcgacggc accaccggct acgccaccga cggcaccccg
     2341 gtcgattgcg tgcgcttcgc cgctttaggc ctcgtggacc agccggacct gatcgtgtcg
     2401 ggcatcaacc acgggtcgaa cctcggtgac gacatcacct actcgggcac ggtcgccgcc
     2461 gcgctcgagg gcgtcgtgct cggcatcccg gcgatcgccg tgtcacagca gtcggaccgc
     2521 cgccagctcg acttccacga cggtggcggg ttcgacttca cacaggtcgc cgcgttcgcg
     2581 gcccgcatcg tggaggagct cgaggacgtg ccgatcccgg cggacacgtt gctgaacatc
     2641 aacgcgcccg cgggggaaat cacgggcgcg cgcgcatgcc ggctcggcaa gcggatctac
     2701 cacgaccgcc tcgagctcga gagcgaggac gacgagggcc ggcgcctata ccggatctac
     2761 gggcgcgagc cgggctacga ggaggacgag ggcacggact tcgccgccat cgccgacggc
     2821 tcgatcgccg tcacccccct gcacttcgac ctgaccgatg aggccgggat cgagcagctg
     2881 tccggattcg agctggagcg cctgctgagg ccggcggcac gagaggttga gtagtccggc
     2941 tgagcgcgcg gcagagctcc gccgcgagat cgagttccac aaccaccggt actacgtgct
     3001 ggacgacccg gtggtctcgg acgccgagta cgacgggctg ctgaacgagc tgcgcgaaat
     3061 cgagcacgag caccccgagc tgctcacgcc ggactcgccg acgcagcggg tcggcgcacc
     3121 gccgctcgac aagttcgagc acgtgcgtca cctgcagccg atgctgtcgc tcgccaacgc
     3181 ccgtggcgag gacgagctcc gcgcctggga gaagcgcgtg cgcaacctcc tcgagaggca
     3241 ggggctcgac gacgggaagc tcgagtacgt gagcgagccg aagatcgacg ggctcgcgat
     3301 ctcgctcgtg tacgagaacg gcgtgctcgc gcgcggcgcg acccgcggcg acggcgagat
     3361 cggcgaggac gtgacgcaga atctgcgcac gatcaaggcg atcccgctgc gggtggagga
     3421 tgccccgcgg ctcttcgagg tgcgcggtga gatctacctg cctcgcagcg ccttcgcccg
     3481 cctgaacgag cagcgcgccg aagcgggcga gccgaccttc gccaacccac gcaactcggc
     3541 ggccgggtcg atccgccagc tcgatcagtc gatcgccgcc tcgcgcccgt tgtcgatctg
     3601 gtgctacggc atcggcgcag tcgagggcat cgagttcaag acacacaagc agtcgctcga
     3661 ctggctgcac gatcgcggct tcaaggtgaa ccccgatgtc caggtgcacg cggatgtgga
     3721 gagcctggcc gcgcgctgcc gcgagtggga ggagatccgc gagtcgctcg actacgagat
     3781 cgacggcgtc gtagcgaagg tgaacgacct ggagttgcag cgcgcgcttg gcgtggtcgg
     3841 gcgagagccg cgcggcgcga tcgcgtggaa gttcccgccg atgacggcca ccaccacgct
     3901 caacgacatc ggctggaacg tggggcggac cggccacatg gtgcccttcg cgatcctcga
     3961 gccggtccag gtctcgggcg tgactgtcaa gctcgcaacc ctccacaacg aggaggacct
     4021 gctgcgcaag gacatccgcc cgggcgacga agtcgtggtg atgcgcgcgg gcgacgtgat
     4081 cccgcaggtg gtctcgccga gcccggccgc gcagcggaac cccaaccgca agccgcgcga
     4141 gtcggcgctg ccgccggaga agtgcccggc gtgcggcact ccgacgatca agcccgaggg
     4201 tggtgtctgg acgatctgcc cgaaccgcga gaactgcccg ggccaggtgc tccaggcggt
     4261 gaagcacttc gtgggcgcga tggacatcga cggcttcggc gaggagaccg cgatccgctt
     4321 cctgcgcgac ggcgtgatcg acggcatagc gggcatctac gagctcgacg cggagaagct
     4381 cacggcgctc gaggggttcg gtgagatctc ggcgcgcaac ctgctcgcca acatcgaggc
     4441 gtcgaagcag cagccgttca accgcgtcct ctacggcctc ggcattcccg gcatcggctg
     4501 ggtgaacgcg cgggcgctcg cctcacgctt ccgctcgatc gacgcgctca tggaggccga
     4561 tgccgagcag atccaggaga ccgacgggat cggcccggtg ctcgcgggca ccatcgaggg
     4621 cacgctgaag gaggagcaca cgcgcgacct ggtccggcgc ctgcgtgagc acggcctcac
     4681 gatggaggag aagggcgagg cggccgcggc ggacgggccg ctcgcgggca agacgatcgt
     4741 cctcactggc acgctgcccg agctctcgcg cgagcgggcc accgagctga tcgagcaggc
     4801 cggcggcaag gtgacgggtt ccgtctccaa gaagaccgac tacgtggtgg ccggcgcgga
     4861 cgccggctcg aagctggcga aggcgcagga gctcggcacc gacgtgctcg acgaagaagg
     4921 cctgcgagcg ctgctgcagg cctgacccgc tctgtcgccg ccggatcggc tttggcttat
     4981 cgtggaactc ggatgggaga acggcacccc gagcgcccgt tctatctcgc cgccgctttc
     5041 ttcctcgtac tcgacctgct gctcattgcg tcgagtagcg ggcagtcctg gcgggtgctt
     5101 ctgcctgtct tcgcgctggt gctgctcttc ctcggcgtga ggcgccggcg cgagcagcgg
     5161 cgcgacgggc accgccgcta ctagccgttg tacgcggcga gcgcgtttgc catgcggtcc
     5221 tgaagcgacc gcgcgccggt cacgcggacg cggttcatca ggaacgagaa cgcgatcagg
     5281 tcgccgcctc gcgactggca gaagcccgag agggcgctca cgttgctgag cgtgccggtc
     5341 ttcgctcggc agcggctctt cgccgctccg ctgcgcatgc ggtcgtgcag cgtcccgtcc
     5401 acccctgcga ccggcagggc gtcgaagaga tcctggaact ccggacgcgc gcgcacgcgg
     5461 tcgagcaggc ccacgaccat gcgaggcgac gcctggttgc cgcgcgcgag ccccgatccg
     5521 tcgacgatcc gggcccgcag tccgagccgc gtcgcaaatg agcgggccgc cctggcgccc
     5581 gccgcggtgg tgcccggggc gcccttgagc gcggcgaggt ccttgagcag cgtctcggcg
     5641 aagaagttgt cggagggctt gatcgtgagc cgcacgagcc gcgacatggg cggcgactgc
     5701 tccgtcgcga tcggggcggc gtcgggcggc gccatgcccg cctgcgcctg accgcgaacg
     5761 ctgatcccgc ggctctcgag cgccttctcg agctgtagcg cggcgtacgc gggcggattg
     5821 gactgaaacc cgcccgtcgc gagcccgcgg ttgtaggaga gcgcgctcaa cgggccgacg
     5881 tatgacgaca ccccgaaatg cgagtcgggc ccgccgcgca gcgagtcgaa gcgcgactcg
     5941 tcgccgagga tgcggccgcg aaccgaggtg atgccggccg cctccagctc gtcggcgagc
     6001 acctccaccg tcgcgccgcc gccgtagttg cgcttcgcga acgcgtcgct gccgaaggtc
     6061 gggtcaccac cacccacgag gtagaggtcg cccggccacg tgccgtccgg ctgcgcctgt
     6121 ccgacgccct tcaccgtcgt cgccaaagtg gcagcgcttc cataggaggc cagcgtcgcg
     6181 gacgtggtga acagcttcgt gttcgaagcg aggatccgtg cgctgtcctg gctggcgcgg
     6241 aagagcaccc gccgactcgt gacatccagc acgtatgcgc ccgagctgcg tccggcgtgg
     6301 cgcatggccg aggccagcac gcggcgcagc ttcgagtctt ggctctgagc cgccgcggcc
     6361 gcgggcaata gcagcagcgc tgcaagtgcg gcgagcaggc aaagacgtcg catcctcggg
     6421 tcgaaacacg gcacggcggg cgagagtagc ggtcgggcca gccggcccct ccgagggctg
     6481 atgcgataat cgaggccggg atgggcaacg ttgtcaagct cgagaaaccc gcacgggggc
     6541 gtgcggcacg gggcggccaa accgcgcgca ggcgccggcg gcgctccaag acggcgctgg
     6601 tgctgggagg cggcggcttc accggcggcg tctacgagat cggcgccctc agggcgctcg
     6661 accttctggc cgtgaaccgc acggtcaacg agttcgacgt ctacgtgggc acgagcgccg
     6721 gctcattcgt ggcgagcctc gcggccaatg gcgtcactcc cgaggagatg atgcgcgtgg
     6781 tgaaccagca ggttcccacg cccttccacg acatcgatct gagcaggctc ctgcggccca
     6841 actacgttga cttcgcgaag agcgccgcgc tgcttccgct ccggatggtc gggattatcc
     6901 gcacgatggc agcgaacctg aactcggttt cgctgatgga cctcgtcgtc gggctcgcgg
     6961 agggcctgcc cgcgggcatc tacaacggct cgggcatcga gggctacctc accgacgtac
     7021 tcggtgacct cgaccgcacg accgacttca gggagctcaa gaaccagctc ttcctcacgg
     7081 cgaccgacct cgacacgtgc gagcggatca tcttcggcga ggacggctgg gacgacatct
     7141 cgatcccgaa ggccgcggcg gcgtcgactg cgctgccaat ggtctacaag ccggtcgagc
     7201 tgaaggggcg gcagctggtg gacggcggca tcggctccac cacgaacctc gacatcgccg
     7261 tcgagaaggg cgcgaagttc gtcgtggtgg tgaacccgct cgtgccctac gtgaacgact
     7321 tccagaagct gatcccgacg atgctcggca gccgcgtgcg ccgggtctcg gacatcggct
     7381 tcccgcagat cggctaccag gcgttcaaga tgctggccca ccagcgcctc cacgaggcgg
     7441 tgcgccagtg ggaggagcgc taccccggcg tcgacatcat cctgatcgag cccgacccgg
     7501 acgacgagct gatgttccag acgaacatcc tcaatttcgc gaagcgcgtg gacatcgctc
     7561 gccacggctt cgagtcggtc acgttcaagc tcgcgaagga ctacgagcgc ttccgctcga
     7621 tctgcgcgaa gcacggcatc gagatctccg ccacgcgcgt gcgcaaggtt atgcgccagc
     7681 tcgagcgtgc ggaggcggag gagactcggg cctggcgccg catcctcgag cagacgacgg
     7741 gcacgctgct gcgccagtcc gaggacgcct gagttagggc gcccgcggtc ctcagcggag
     7801 ccgcgcgcct agcgcgatca gcagcacgac caccgcgagc gcgatcacga actcggccag
     7861 cccgctgacg gccgccgccg gcggggtcat cgtgcgacgc cggcgagcgc ctcgagcagg
     7921 cgctcggttg cggcagcccc gcggatggcg gcacgcacgt ggtggtcgtc gccgagaggc
     7981 ccgcccggcg ccaccagcac gccggcgcgc tcgagcctcg cggccagctc cgagccggtg
     8041 aggcccggcg ctcgcagcca gacgaagttc gcctggctgt ccggtgcgtc gaccggcatg
     8101 tcgtggagcg cgtcgagcag gcgcttgcgc tggacgagca cgagcatccg ccggcgccgc
     8161 acctcgtcgt cgccgatgcg cagcgcctgg ctcatgacag cctgcgtgag cgcattcacg
     8221 ccgagcaccg gtgctatcgc gtcgagcagg cgcgccgtgc ccgtcgagcc gacggcgtag
     8281 ccggcgcgca gtccggagag cccatagacc ttcgagaacg tgcggaacac gagcacgcgc
     8341 gggtgctcgt ccgccaggcg cagcaccgcg tcctcgtcct ccacgtcctg gaactggaca
     8401 taggcctcat ccacgagcaa gtgggcgtcg ccggggaggg cggcggcgag gcgcgccacc
     8461 tcggcggccg gcaagtaggt gccggtgggg tcgttcggat tgcacagcac gacgacgcgc
     8521 gtgcggtcgc cgcaggcggc gatcagcgcg tccacct
//
LOCUS       DASVXS010000187         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_333278, whole genome shotgun sequence.
ACCESSION   DASVXS010000187 DASVXS010000000
VERSION     DASVXS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333278"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VF752_10365"
     CDS             complement(<1..539)
                     /locus_tag="VF752_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8855993.1"
                     /translation="MEASALRQTAQLPALAGRSPLLRLQSDEKLVALIRRGHHGAFEA
                     LFHRYRSRLLAFCRHMLGSTEDAEDVLQEVFTAAFNAICADDRPINARPWLYRIARNR
                     CLNHLRRPVHPGQDSMDVFENDGGSSAADTVHRREEFRQIVADVQDLPETQRTALLLR
                     EIDALSYDQIAEAMDTTIP"
     gene            687..1028
                     /locus_tag="VF752_10370"
     CDS             687..1028
                     /locus_tag="VF752_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855994.1"
                     /translation="MCAQRDDRHHFVRLQVFLPRHRLDRDLADGADSGSTAAHARRAE
                     QLERRRRGLAVIVGQMIDAAERPPERGMGSGMKFQVEQIRASRTPLCTVRRNGRSRDL
                     DLACPPGWRNW"
     gene            1007..1091
                     /locus_tag="VF752_10375"
     tRNA            1007..1091
                     /locus_tag="VF752_10375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1041..1043,aa:Leu,seq:taa)
     gene            1207..3297
                     /locus_tag="VF752_10380"
     CDS             1207..3297
                     /locus_tag="VF752_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855995.1"
                     /translation="MLASILLVLAVVVGYARVAVFDADQFANRATASLQKKSVRDLAA
                     QRITDQLVLKNESDLIAARPAIEAAAAAIVGGDAFASLFRPAVKDVHRAIFDRDQHTF
                     TLTLADVGTVVASVLQRIDPAAAKKAEQNRDVVLVKRDIGSATSDLARVAERVRALAA
                     ILGGLTLLLMAAAIVISPDRRRTVTRLAMGALAGGVLIVIVYAVGRSILLSRVTGSDE
                     RDAAGAIFDTFASGLRTAGWIMAGAGAVVAAAASSLMKPMDVEHAVARAWGWVIREPE
                     TTPMKVMRAVLLIALGVWIVLEPSTVLQVVATLAGVYVVFKGVEAILRLVYQEPEERA
                     APVPKPKRRHRARRVAALAIVGVLIAGALGAFTTSDALNKAAPAIVGCNGSKALCGKP
                     FDKVVLPATHNAMSVPLPGWYSAEQEHPIATQLADGIRGLLIDTHYADKLPNGKFRTE
                     IKNEQDLQERIKEDGISPEALAAAKRLRERLGFEGSGKRGMYLCHSFCELGATPLESV
                     LSDIHDFLVTRPDAVLLVINQDYVTPQDFVKAVEDAGLTQFVYKGPVSGRWPTLREMV
                     RDNQRLVLLAENHAGGAPWYHPAYQSITEETPYDFKETAELTQSSQLPARCRANRGPE
                     HAPLFLINHWISTDPVPLPSNAATVNAYQPLLHRAEVCERLRHHLPNLLAVNFYKEGD
                     LFKVVDKLNGVTKR"
     gene            complement(3294..>3851)
                     /locus_tag="VF752_10385"
     CDS             complement(3294..>3851)
                     /locus_tag="VF752_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8855996.1"
                     /translation="CNQIYEIEEGRPFWKLRPLQIVMTVLMIILLAIVLIGLVVSGKL
                     AHAIGDQIGLGNTAVTVWNIAKWPILLGIVMFMFAMLYYAAPNVRQPKFRWITPGGVL
                     AVLIWIAASALFAVYVSQFGSYNKTYGTLGGVIIFLIWLWISNIAVLLGAEFDAELER
                     ERELQAGLPADRELQLPPRSEPKNA"
BASE COUNT          630 a         1291 c         1325 g          605 t
ORIGIN      
        1 ctcgggatcg tcgtgtccat cgcctcggcg atctggtcgt acgaaagcgc gtcgatctcg
       61 cgcagtagca gcgcggtccg ctgtgtctcg ggcaggtcct ggacgtcggc gacgatctgg
      121 cggaactcct cgcggcgatg aacggtgtcg gcggctgagg agccgccgtc gttctcgaac
      181 acgtccatcg agtcctggcc cgggtgcacc ggccgccgca ggtggttcag gcagcggttg
      241 cgcgcgatgc ggtagagcca cgggcgcgcg ttgataggcc ggtcgtcggc gcagatcgcg
      301 ttgaacgccg cggtgaacac ctcctgcagc acgtcctccg cgtcctcggt cgagccgagc
      361 atgtgtcggc agaaggcgag cagccgcgag cggtagcggt ggaacagcgc ctcgaacgcg
      421 ccgtgatgtc cacgacgaat gagagcgacg agtttctcgt cgctctgcag cctgagcagc
      481 ggcgaacgcc cggcgagcgc aggtaactgc gcggtctgtc gtagagcgga agcttccaaa
      541 tccctggcga ggggtctacg tcagccaaga caagctagca gcggaaaagt tgcgcgagcc
      601 gccgtgggca acgggctcga tgagatcttc acgtgctcac ggcactcctg cacgccgcct
      661 tcacatgctg tgagcacact gaatgcatgt gcgcacagcg agacgaccgg caccacttcg
      721 tccgcctgca agtgttcttg ccccggcacc ggctcgaccg cgacttggct gacggagcgg
      781 actccggctc gacggccgcg cacgcgcggc gcgccgagca gctcgaacgc cgccgtcgtg
      841 ggctcgccgt gatcgtcggg cagatgatcg acgcggcgga gcgcccgccg gagcgcggaa
      901 tgggctcggg gatgaagttt caggtggagc agatccgggc gagccgcacg ccgctctgca
      961 cggttcgccg aaacggtcgc tcgcgcgact tagacttggc gtgcccgccc gggtggcgga
     1021 actggtagac gcgggggcct taaaagccct ggctctcaac gagcgtgggg gttcgagtcc
     1081 ctccccgggc actgcctgcg gttaggaggc actctggccg gatcgattgc gactcgcttc
     1141 tcggcttggg cggacgcaac gcccgagcgg atcgcgcgcc gcggccgcgc ggcgagcgtg
     1201 ctggtagtgc tcgcgtcgat cctgctcgtg cttgccgtcg tcgtcggcta cgcgcgcgtc
     1261 gctgtgttcg atgcggacca gttcgcgaac cgcgcgacgg cctcactgca gaagaagagc
     1321 gttcgcgacc tggccgcgca gcggatcacc gaccagctcg tgttgaagaa cgagagcgac
     1381 ctgatcgccg cccgcccagc gatcgaggcg gccgccgccg cgatcgtcgg cggcgacgcg
     1441 ttcgcctcgc tcttccgccc ggccgtgaag gacgtgcacc gcgcgatctt cgaccgcgac
     1501 cagcacacgt tcacgctgac gcttgcggac gtcgggaccg tggtggcctc ggtgctccag
     1561 aggatcgacc ctgccgctgc gaagaaggcc gagcagaacc gcgatgtcgt gctcgtcaag
     1621 cgagacatcg gcagcgcaac gagcgacctc gcgcgggtgg ctgagcgggt gcgggcgctg
     1681 gcggcgatcc tgggcgggct gacgctgctg ctgatggccg cagccatcgt catcagtcct
     1741 gatcggcgcc ggaccgtgac ccggctggcg atgggcgcgc tggccggcgg agtgctgatc
     1801 gtgatcgtgt acgcagtggg gcgctcgatc ctcctgtcgc gcgtgaccgg ctccgacgag
     1861 cgggacgcgg ccggtgccat cttcgacacc ttcgcgagcg gcctccgcac cgcgggctgg
     1921 atcatggccg gcgccggagc cgtggtcgcg gcggccgcct cctcgctgat gaagccgatg
     1981 gacgtcgagc acgccgtcgc ccgcgcgtgg ggatgggtca tacgcgagcc cgagaccacg
     2041 ccgatgaagg tcatgcgcgc cgtgctgctg atcgcgctcg gtgtgtggat cgtgctcgag
     2101 ccaagcaccg tgctccaggt cgttgccacg ctcgccgggg tgtacgtggt cttcaaggga
     2161 gtcgaggcga tcctgcggct cgtctaccag gagcccgagg agcgcgccgc gcccgttccc
     2221 aagccgaagc ggcgccatcg cgctcgccgc gttgccgcgc tcgccatcgt gggcgtgctg
     2281 atcgcgggcg ccttgggcgc gttcaccacg tccgacgcgc tgaacaaggc ggctccggcg
     2341 atcgtcggct gcaacggctc gaaggcgctg tgcggcaagc ccttcgacaa ggtggtgttg
     2401 ccggcaacgc acaacgcgat gtcggtgccg ctgcccggct ggtactcggc ggagcaggag
     2461 cacccgatcg cgacccagct cgccgacggc atccgcggcc tgctgatcga cacgcactat
     2521 gcggacaagc tgccgaacgg caagttccga actgagatca agaacgagca ggacctccag
     2581 gagcggatca aggaggacgg catcagcccc gaagcgcttg ccgcggccaa gcggcttcgc
     2641 gagcggctcg gcttcgaggg gagcggcaag cgtgggatgt acctctgcca cagcttttgc
     2701 gagctcggcg cgacacctct cgagagcgtg ctctccgata tccacgactt cctcgtgacg
     2761 cggccggacg cggtgctcct cgtgatcaac caggactacg tgacgcctca ggacttcgtc
     2821 aaggcggttg aggacgccgg cctcacccag ttcgtctaca aaggcccggt gtccggccgc
     2881 tggccaacgc tccgcgagat ggtgcgcgac aaccagcggc tcgtgctgct tgccgagaac
     2941 catgcgggcg gagcgccctg gtaccaccct gcctaccaga gcatcaccga ggagacgccc
     3001 tacgacttca aggagacggc cgagctcacc cagtcgtcgc agctgccggc gagatgccgc
     3061 gcgaaccgcg ggccggagca cgcgccgctc ttcctcatca accactggat cagcacggat
     3121 cccgtgccgc tgccgagcaa cgccgcgacc gtgaacgctt atcagccgct gctccaccgg
     3181 gcggaggtct gcgagcggct gcgccaccac cttccgaacc tgctcgcggt gaacttctac
     3241 aaggagggcg acctcttcaa ggtcgtcgac aagctcaacg gagtgaccaa gcgctaggcg
     3301 ttcttcggct cgctgcgcgg cggcagctgt agctcgcggt cggcgggtag gccggcctgc
     3361 agctcgcgct cacgctcgag ctcggcgtcg aactcggcac cgagcagcac ggcgatgttc
     3421 gagatccaca gccagatgag gaagatgatc acgccgccga gcgtgccgta cgtcttgttg
     3481 taggagccga actggctcac gtacaccgcg aacagcgccg acgccgcgat ccagataagc
     3541 accgcgagta cgccgccggg ggtgatccag cggaacttcg gctgcctcac gttcggcgcg
     3601 gcgtagtaga gcatcgcgaa catgaacatc acgatcccga gcaggattgg ccacttcgcg
     3661 atgttccaga cagttaccgc cgtgttgccc aggccgatct ggtcgccgat ggcatgtgcg
     3721 agcttgccgc tcaccacgag accgatcagc acgatcgcca gcaggatgat catcaggacc
     3781 gtcatgacga tctgcagcgg ccggagcttc cagaacggcc gaccctcttc gatctcgtag
     3841 atctgattgc a
//
LOCUS       DASVXS010000188         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1183178, whole genome shotgun sequence.
ACCESSION   DASVXS010000188 DASVXS010000000
VERSION     DASVXS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183178"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VF752_10390"
     CDS             <1..454
                     /locus_tag="VF752_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8855997.1"
                     /translation="QRGLSPGERGDTPTDGGHASAGHFCPPTLVTDLPSDSPVLREEI
                     FGPLLAVERFPSVESALDAMASAPFALTGGLYSRNPRTVEEVTRRAPVGNLYVNRHIT
                     GAMVARQPFGGNRRSGIGSKAGGPDYLLQFVEPRVVSENTMRHGLVVE"
     gene            608..1051
                     /locus_tag="VF752_10395"
     CDS             608..1051
                     /locus_tag="VF752_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8855998.1"
                     /translation="MKRWSLIAVVASALIAASTTFAADRSGRDRETGVAFRLSGRYLA
                     VQAERRSPRFGPVGTPTPPLAHKVWRAACGVSTDDPSAVASAVRRWPRGRHVLRFHLN
                     RDVSKHARWCLIESRHDGADYAVANMRRSRAPRARAIGSKARAPD"
     gene            1110..1715
                     /locus_tag="VF752_10400"
     CDS             1110..1715
                     /locus_tag="VF752_10400"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8855999.1"
                     /translation="MIEYASVTPLVMHDSTAEAVLYVPVGVFAVGELAIGARSRPRIR
                     KSEDRGTYWIWALLYGLGIVLAIRASDGVDSLTISGGWGPVIVGGLLMCAGIALRWWS
                     VVTLGRFFQVVVVIQEGHKLVQNGPYRFLRHPAYTGALMVAFGLGIGLANWLSVLATI
                     VLPLAAVLIRVHVEEEALQKRFGAEYDAYAARTARLIPRVW"
     gene            1750..2214
                     /locus_tag="VF752_10405"
     CDS             1750..2214
                     /locus_tag="VF752_10405"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEX8856000.1"
                     /translation="MPTFAIQPISDELADHVRTNRSDPVWEHPAHECVPDAGPCRSCL
                     GSVAGEPTLLFTHDSFAGRGEFKMPGPVYIHAERCEPYSDVSRFPEWALGKPLTFEAY
                     GPGVTLMAVERATGDSGAEIIDHLLAEHPDAEYVNVRNTGAGCFLLRADRAG"
     gene            2250..2648
                     /locus_tag="VF752_10410"
     CDS             2250..2648
                     /locus_tag="VF752_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856001.1"
                     /translation="MSEKSLAVVQRCFELADKGGARAVVEVLDPDVEWDVSRNSPETK
                     VWRGRQGFVDGWRFWMRDWSEFRVELDELIDAGDVVVALVRQSGRGRGSGVEVEQETA
                     TVFTVEGGKITRIEAYGGRREALRAAGIPA"
BASE COUNT          392 a          920 c          944 g          440 t
ORIGIN      
        1 gcagcgtggt ctgtcccccg gggaaagggg tgacaccccg acagacgggg gacacgcatc
       61 cgccggccac ttctgcccgc ccacgctcgt gaccgatctg ccttcggact cgcccgtcct
      121 ccgcgaggag atcttcggcc cactgctcgc ggtcgagcgc ttcccctctg tggaatccgc
      181 gctcgacgcg atggcgagcg cgccgttcgc gctcaccggc gggctctatt cacgcaaccc
      241 gcgcaccgtc gaagaggtca cgcgccgcgc gccggtcggc aacctctacg tgaaccgcca
      301 catcaccggc gcgatggtgg cccgccagcc gttcggcggc aaccgccggt cgggcatcgg
      361 ctcgaaggca ggcgggcccg actacctgct gcagtttgtc gagccgcgcg tcgtgtccga
      421 gaacacgatg cggcacgggc tggtggtgga gtaggtcccg ccgctacttg acgcgcacga
      481 agtacggcga gccgcccgtc tcccgctgcg cgcaagcaag tcgctatgcc cgcgtggcca
      541 ccgatgaatc ggggaggacg cttggaccca tggtcgaata gacgacgatg ctcgtttcgg
      601 gcgtctcgtg aagcgctggt cgttgatcgc cgtggtcgct tctgcgctga tcgcagcgtc
      661 gacgacattc gccgccgacc gttcaggccg cgaccgtgag actggtgttg cgttccgcct
      721 gagcggtcgc tatttggcgg tgcaggccga gcgccggagc ccgcgctttg gaccggttgg
      781 aacgcctacc ccgccgctcg cccacaaagt ctggcgcgcg gcctgcgggg tcagcacgga
      841 cgatccgagt gccgttgcga gcgcggtgcg tcgctggccg cgcggacggc acgtgcttcg
      901 cttccacctg aatcgcgacg tgtcgaagca cgcgcgctgg tgcctcatcg agtcccggca
      961 cgacggcgcc gactacgcgg tcgccaacat gcgccgctcg agagcgcctc gcgcccgggc
     1021 catcggctcg aaggcgcgcg cgccggacta gctggcgtag ccatcgtctg tgatcgcgtc
     1081 gcgcaacggg tgtccgagcg tcgacgcaca tgatcgaata cgccagcgtg actccgctcg
     1141 tgatgcacga ctccaccgcc gaagcggtgc tgtacgtgcc ggtcggtgta ttcgccgtcg
     1201 gcgagctcgc gatcggcgcg cgttcgaggc cgcgtattcg gaagagcgag gaccggggca
     1261 cgtactggat ctgggcgctg ctgtacggct tggggatcgt gcttgcgatc cgggcgtccg
     1321 acggcgtcga ctcgctcacc atcagcggcg gctgggggcc ggtcatcgtc ggtggtctgc
     1381 tcatgtgcgc cggcattgcc ctgcgctggt ggagcgtcgt cacgctcggg cgcttcttcc
     1441 aggtggtcgt cgtgatccag gagggacaca agctcgttca gaacggcccg taccgcttcc
     1501 ttcgtcaccc cgcctacacc ggagcgctga tggtcgcgtt cgggctgggg atcgggctcg
     1561 ccaactggct gagcgtgctt gccacgatcg tgctgcccct ggccgccgtc ctgattcgcg
     1621 tccatgtgga ggaggaggcg ctccagaagc gcttcggcgc tgagtacgac gcctacgcgg
     1681 cgcgcaccgc ccgcttgatc ccgcgggtct ggtagctcac aaatcgcgcc ggccgggcgt
     1741 tctacgccca tgccgacatt tgcgatccaa cccatcagcg acgagctcgc cgaccacgtc
     1801 cgcaccaacc gcagcgaccc cgtgtgggaa cacccggcgc acgagtgcgt tccggatgcc
     1861 ggcccttgca ggtcgtgtct cggctcggtg gccggcgagc cgacgctgct cttcacacac
     1921 gactcgttcg cgggccgcgg cgagttcaag atgcccggcc cggtctacat ccacgccgag
     1981 cgctgcgagc cctactcgga cgtcagccgc ttccccgagt gggcgcttgg caagccgctc
     2041 accttcgagg cctacgggcc gggcgtcaca ctcatggccg tagagcgagc gacaggcgac
     2101 agcggcgccg agatcatcga ccacctgctg gcggagcacc ccgacgcgga gtacgtgaac
     2161 gtccggaaca ccggtgcggg ctgcttcctg ctccgcgccg accgcgccgg ctaggaccgc
     2221 agccggcgcg ccgttacgct agctccgccg tgtcggagaa gagcctcgca gttgtccaga
     2281 ggtgtttcga gctcgcagat aagggcggcg ccagggcggt ggttgaggtc ctcgacccgg
     2341 acgtcgagtg ggatgtgtcg cgcaacagcc ccgagacgaa ggtgtggcga ggcaggcaag
     2401 gattcgtgga cggctggcgc ttttggatgc gcgactggag cgagtttcgg gtcgagctcg
     2461 acgagctgat cgacgccggc gacgtcgtcg ttgcgctggt tcgccaatct ggccggggac
     2521 gcgggagtgg ggtcgaggtc gagcaggaga ccgcgaccgt gttcaccgtg gagggcggaa
     2581 agatcacgcg catcgaggcc tacggcggca ggcgcgaggc gctacgcgcc gccggcatcc
     2641 cggcctagtc gcagaagaag gtgacctgcg gcgacgtcgg cttgccgctc accgcg
//
LOCUS       DASVXS010000189        13009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_50511, whole genome shotgun sequence.
ACCESSION   DASVXS010000189 DASVXS010000000
VERSION     DASVXS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50511"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VF752_10415"
     CDS             complement(<1..1563)
                     /locus_tag="VF752_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX8856002.1"
                     /translation="MRKRVIVTVDRGETRVAILEAEGEPTPRGTAAEQDKAATGSKRG
                     RRGGRGRPRAKSPSLARDERVGELYIERRGSRSIVGNIYKGKVDNVLPGLEAAFVDIG
                     LEKNGFLHVDDIVMPGVEIQRRGRGGKGQRISELLKPGQEIVVQVMKDPLKTKGARLT
                     MQLSIAGRYLVYAPTAEGVGVSRRLDDKERDRLRKETRGLDLKEGGAIIRTAARGAKR
                     EDFEREVKYLHKLYEVMQKRAKETVAPAMVFQEADLSIRVVRDIFSAHFEKAIVDDAK
                     QHQRLTSFFTRTAPELVDRVELWEGNTDLFEANGVDKVIEGMLTRRVDLPSGGYLMID
                     YGEALTVIDINSGSFTGRGKSARLEDVITKTNLEAAEEVVNQLRLRDIGGIIVIDSID
                     MARARNRDAVLKTLRKSLDEDRTKTFVVEISPLGLVEMTRQNVTDGVREIMTKTCPVC
                     HGEGVVKSEETVAIEIARHMRHAVADYAEALTVIDVNTGSFTGKGKAARLEDTITKTN
                     LEAAEEVVSQLRL"
     gene            complement(1833..3032)
                     /gene="rodA"
                     /locus_tag="VF752_10420"
     CDS             complement(1833..3032)
                     /gene="rodA"
                     /locus_tag="VF752_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX8856003.1"
                     /translation="MQAGLGTTVRDFREPGPGPGLRLLRQDPLLLLATLGIVGASIYT
                     IWTATNTDIPGDPTYYVLRQSLYAGVGLLLMFVIANFDYSRLREWRIGIYTTMIASIV
                     IVFALAGATRGSKRWISFPFFNFQPSELGKLLLILALAGFLVDRTRRMSDRDTTRRIM
                     LLALLPAILVIAQPDLGSGLVYITIACALLFVAGTKWTHFAVLGFLGAAAVTIVLVAA
                     PAAGVHVLKDYQVQRLTAFINPSHDPRDQGYQLNQSITAIGSGEKTGRGPEGATQTKL
                     DFIPEHHTDFIFSVVGEEFGFVGCAFVLSLYALLIWRGLRILTTSKNLYGSLLAGGIL
                     AMIMFQLFVNVGMTLGIMPITGVPLPLMSYGGSSVLVTLLCLGVLQAVHVQARETAVS
                     KGRGSLL"
     gene            complement(3073..4947)
                     /gene="mrdA"
                     /locus_tag="VF752_10425"
     CDS             complement(3073..4947)
                     /gene="mrdA"
                     /locus_tag="VF752_10425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8856004.1"
                     /translation="MYRDTDHRPAITPQLALRVAILGGFALVMFGVIFFRLWYLQILS
                     GDKYLADANNNRVREIRVDAPRGKIVDRNGNVLVDNRPGRAIVVRPDKLPPDRKSREM
                     LYKRLSPVVGQPWREIRKNVATQLKAVPFSRAIVKQDATQAQFSYVLEHQEQLAGVDV
                     ERIFLRRYPHRSIGAHLFGTVGEVTSQELKDKQRYRGVVQGDRVGQSGIEYSYDRYLR
                     GQDGADRVQVDSTGTLRGTLAERNPVQGHQLRLSLDFNTQRIGQAALNGRRGAFVVMD
                     IKTGEVRALGSSPSFDPNIFAKVIKKKDYDALTSAANGSPLSNRAIQGLYPTGSTFKL
                     ITSVAALQSGVITPDTPQYDPGFFKLGPTTFYNAGKTGHGTLALRDAIKVSSDVFFYK
                     LGAELNNVGNGHVLQSWARKLGLGHSTGIDLPAEQSGLVPSPEWRDRLFKKKLTDRPW
                     SAGDNVNLAVGQGDLQADPLQMAVAYASIANGGYIVRPHLGMRIEDSEGRAIQEFGTP
                     TRRRVSVRPEFRQAILDGLFMAANQPGGTSADVFKDFPIKVAGKTGTAEKGLGRPDQS
                     WYIGLAPYPNPRYVVAVTDEQGGFGAETAAPDARRILAALFGIRSQENKVVKGASRTR
                     "
     gene            complement(4953..5540)
                     /gene="mreD"
                     /locus_tag="VF752_10430"
     CDS             complement(4953..5540)
                     /gene="mreD"
                     /locus_tag="VF752_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX8856005.1"
                     /translation="MIVTPGAIVRLGVLALVTVLLDISGFSGIHVLGGTADLVPLLVG
                     AVALYGGALAGAGTGFATGLLLDLLLGQSLGLSSLVLTLVGYWVGSYRELRDSGHGLL
                     PLVVGAAASVGYVVGIAIVSFMLETNSSVSVLALRSMLLTIFLNTLIAMPFFILVRRV
                     LRPVLVVDPLERRRRRRTVAGESGPIGLRGLEIGR"
     gene            complement(5537..6424)
                     /gene="mreC"
                     /locus_tag="VF752_10435"
     CDS             complement(5537..6424)
                     /gene="mreC"
                     /locus_tag="VF752_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX8856006.1"
                     /translation="MYDKKVVRRRRAALAVLVGLSLALLTVYFGESTGGALHAIQRGA
                     QTAFAPIESGLSKVLKPFRDIAGWTGDVVHAKGENKRLRTENAQLRAQAAQGQVATRD
                     NEQFRALLGLPKKAGYPASAKPVTARVIARSPTLWYSTVDIDKGSSDGVRMNQPVVTG
                     NGLAGKITSVTGGNARVTLITDGSSAVSAEVVPLGASGIVTPQVGNPNDLLLDYIERG
                     RDIVKGQTVVTSGFTSSKLESLFPKGIPIGRVTRVEPGELELYQRVHIQPFADLRRMD
                     YVQVLTGTSGNRSRVVSAR"
     gene            complement(6446..7492)
                     /locus_tag="VF752_10440"
     CDS             complement(6446..7492)
                     /locus_tag="VF752_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8856007.1"
                     /translation="MGFFSYLTGFGGRDMAVDLGTANTLVYVRGRGIVLSEPSVVAVD
                     NRTGEVHAVGIEAKRMLGRTPGTISAIRPLKDGVIADFDVTEQMLRHFIQKVHQNRWA
                     HPRVVVCVPSGVTGVEKRAVEEACLSAGARQAYLIEEPMAAAIGAGLPVGEPTGNMVV
                     DIGGGTSEVAVISLGGIVVSQSIRIGGDELDEAIINYVKREYKLMIGQQTAEEVKLEI
                     GSAFPLEEEVQAEIRGRDLVSGLPKTVVLTSEEVRMALEEPLQAIIDAVKETLDRTPP
                     ELAADIMDRGIMLAGGGSLLQGLDERLRDETQMPAHLAESPLTCVAVGSGRSLEEFEA
                     IHRSNRNSRNRRRY"
     gene            complement(7662..8219)
                     /locus_tag="VF752_10445"
     CDS             complement(7662..8219)
                     /locus_tag="VF752_10445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX8856008.1"
                     /translation="MAPKLILASRSPQRRAILEQLGIEFEVSVPDVEEVSGGDPREGV
                     VENALRKARAVDGDCVLAADTAVVLGGQWYGKPADEREAESYLRTLSGRTHQVWSGIV
                     LRDRDNERTGHAVTQVTFRQLEPADLRWYLDSGEWCERAGAYAIQGRGSALVEEIDGD
                     FWNVVGLPVAELVRLAPDVVRNGMP"
     gene            complement(8364..8768)
                     /gene="ndk"
                     /locus_tag="VF752_10450"
     CDS             complement(8364..8768)
                     /gene="ndk"
                     /locus_tag="VF752_10450"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8856009.1"
                     /translation="MERTLILVKPDAFARGLTGEVIARFERKGLRIVALKHMQVERDL
                     AERHYAEHSEKPFFGDLVEFITGGPLVAMVLEGYEAIRAARQVIGATNPLEAAPGSIR
                     GDYALEVQTNLVHGSDGPESSAREVELFFPEL"
     gene            complement(8799..9374)
                     /locus_tag="VF752_10455"
     CDS             complement(8799..9374)
                     /locus_tag="VF752_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX8856010.1"
                     /translation="MPVAIFGIHNDGLNRAVSIIVLCLVVIWLALVWWTYADARRRID
                     DPMLVGCAALASLFFPFIGTIVYMIVRPPEYLDDVRERELEIAAAQARLHKLDPSCPY
                     CGFEVENTFLRCPSCLRRLKEPCRTCGKPLDPRWKICPYCESEIGQAPPERQRLRPRR
                     QTTAAARVGDDPTITRAASQRPPAGGERPAR"
     gene            complement(9549..10784)
                     /locus_tag="VF752_10460"
     CDS             complement(9549..10784)
                     /locus_tag="VF752_10460"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX8856011.1"
                     /translation="MDERQAESYLLDLELFGMRFGLDRMHKLMTVLGMPQRRFASIHV
                     VGTNGKSSTVRMIAAILERHGIRTGSYTSPHLVSFTERIEVDERPVSGADFAASVSRV
                     QHAAELVNRTQEAGDTVTQFEALTAAAYHELARRGVEVAVVEAGLGGRYDATNVIPSR
                     VQVITNVGLEHTRWLGPTVKDIAEEKLAVVRDQATLVAGDLDADAEAVAERVVRERHA
                     RLVHAPATTHLPLAALGGFQRRNFALAEAAAQAFLGELDPEAVRRAAAETQVPGRMQK
                     VADDPLTIYDGAHNPSGSEALADALPDVVGARRLVGVVGILDDKDAAGMLRPLLPLFD
                     SVIFTRSANPRSLSPATLESLAEKLGGPPVENVADPQAAVSRARELAGPGGAVLATGS
                     IYLVADLVRDRSALRASML"
     gene            complement(10790..>13009)
                     /locus_tag="VF752_10465"
     CDS             complement(10790..>13009)
                     /locus_tag="VF752_10465"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX8856012.1"
                     /translation="RLRPHGGDRYRSGARLERVVDRVQPVLDPPYAHAVQKVFVDLYD
                     KGLIYRDNRMVNWDPGSRSAISDLEVEDREVTDTLYYIDYPLESGRGSVTIATVRPET
                     MLADTALAVHPDDDRYTRLIGETAILPLVGRRLKIIADPYVKPEFGTGALKITPGHDP
                     NDFEIGRTHGLDEVTVIGEDGLMTEAAGERFAGLKVLDARDAVVAALEAEGAIAGTEP
                     YEHDVPHSHRSGMRIEPLISLQWFCDMEKLAEPAIRVVKEGRVRFHPENPWAGVYLSW
                     MENIRPWCVSRQLWWGHQIPVWYCDECGEMIAAEEEPEQCPCGKGSPRRDPDVLDTWF
                     SSALWPFATLRWPADTPELRAFYPTDVLVTARDIIFLWVARMIMMGIEFTGEIPFTDV
                     PITSVIQAPDGRRMSKSLGTGIDPLDEIDEYGADAVRFGLIAMASTQDVRYSEKRVKQ
                     GQDLANKLWNASRLVLLKVDPDVEPAPRPETAEDRWILSRLERATERIGGRYESFELS
                     RAALDLYDFFWGEVCDWYLELVKPRLYDEQADRTALSATLLHVLERVLKLAHPLIPFV
                     TEEIWSFLPGERGLLAASPWPKVDPERFDEAAEGQVGSVIEAITALRRYRDEVGAPAG
                     ARIPGRILAAGYEGALDHVARLARFELEPGSDENGDVVATVAIPRGSVQVLRSDAIDT
                     GEAERRREAEVKRLQSEIARAEGKLANSGFVSKAPEHVVEAERQKLARLKAELEELS"
BASE COUNT         2006 a         4492 c         4432 g         2079 t
ORIGIN      
        1 cagccgcagc tggctcacga cctcctcggc ggcctcgagg ttcgtcttgg tgatggtgtc
       61 ctcgagccgg gccgccttgc ccttgccggt gaacgagccc gtgttgacgt cgatgaccgt
      121 cagggcctcg gcgtagtcgg cgacggcgtg gcgcatgtgg cgggcgattt cgatcgcgac
      181 ggtctcctcg gacttgacca cgccctcgcc gtggcagacc gggcacgtct tggtcatgat
      241 ctcgcgcacg ccgtccgtga cgttctggcg cgtcatctcg acgaggccga gcggggagat
      301 ctcgaccacg aaggtcttcg tgcggtcctc gtcgagcgac ttgcgcagcg tcttgagcac
      361 ggcgtcgcgg ttgcgcgcgc gggccatgtc gatggagtcg atgacgatga tgccgccgat
      421 gtcgcgcagc cgcagctggt tgacgacctc ctccgccgcc tcgaggttcg tcttggtgat
      481 cacgtcctcg agccgggccg acttgccgcg accggtgaag gagcccgagt tgatgtcgat
      541 gaccgtgagc gcctcgccgt agtcgatcat caggtagccg ccgctcggca ggtcgacgcg
      601 gcgggtgagc atgccctcga tgaccttgtc gaccccgttg gcctcgaaca ggtcggtgtt
      661 cccctcccac agctcgacgc ggtcgacgag ctcgggcgcg gtgcgcgtga agaacgaggt
      721 cagccgctgg tgctgcttgg cgtcgtcgac gatcgccttc tcgaagtgcg cggagaagat
      781 gtcgcgcacg acgcggatcg acaggtcggc ctcctggaag accatcgccg gggcgaccgt
      841 ctccttggcg cgcttctgca tgacctcgta cagcttgtgg aggtacttca cctcgcgctc
      901 gaagtcctcg cgcttcgccc cgcgggcggc cgtgcggatg atcgcgccac cctccttgag
      961 gtcgaggccg cgcgtctcct tgcgaaggcg gtcgcgctcc ttgtcgtcga gccggcgcga
     1021 cacgccgaca ccctcagcgg tgggcgcgta tacgaggtag cggccggcga tcgagagctg
     1081 catggtcaga cgcgcgccct tggtcttgag cgggtccttc atcacctgca cgacgatctc
     1141 ctggccgggc ttgagcagct cggagatgcg ctggcccttg ccgccgcggc cgcggcgctg
     1201 gatctcgacg cccggcatca cgatgtcgtc gacgtggagg aacccgttct tctcgaggcc
     1261 gatgtcgacg aacgccgcct cgaggccggg aaggacgttg tcgaccttcc ccttgtagat
     1321 gtttcccacg atcgagcggc tgccgcgccg ctcgatgtag agctccccta cccgctcgtc
     1381 ccgagcgagg ctgggagact tcgcgcgcgg gcggccgcgg cccccgcggc gtccgcgctt
     1441 gctgccagtg gcagccttgt cctgctcggc cgctgtgccg cgaggcgtag gctcaccctc
     1501 cgcctccagg attgcgaccc gcgtttcgcc acggtcgacc gtgacgatga ctcgctttct
     1561 caaaagtgcc tctcataaca atcaagcccg ctgggccccg ctcgtagagc ggcgaccggg
     1621 cgctggcgcc gcgaggcgcc gcgcggtccg caggcgattt acgacccgaa ccggagcgga
     1681 gggcgcgagc ccgtgtgacg gccctatacg cgacttcgga tgcgggttct atgtgctgga
     1741 gctgaacgct caaaagactg tggacgccga tttcggcgaa taaccccggt tccctgcgat
     1801 ccagggcgaa gacgaagtct tctacacaga gtctagagaa gggatccgcg acccttcgac
     1861 accgcggtct cgcgcgcctg cacgtgcacc gcctgaagca cgccaaggca cagcagcgtg
     1921 acgagcaccg aggagccgcc gtagctcatc agcggaagcg gcacgccggt gatgggcatg
     1981 atcccgagcg tcatgcccac gttcacgaac agctggaaca tgatcatcgc gaggatgccg
     2041 cccgcgagta acgagccgta caggttcttc gacgtcgtga ggatccgcag ccctctccag
     2101 atgagcaggg cgtagagcga gagcacgaac gcacagccca cgaagccgaa ctcctctccc
     2161 accaccgaga agatgaagtc ggtgtggtgc tcggggatga agtcgagctt cgtctgcgtg
     2221 gcgccctccg gtccgcggcc cgtcttctcg ccagagccga tcgccgtgat cgattggttc
     2281 agctgatagc cctgatcgcg cgggtcgtgg ctcgggttga tgaacgccgt gaggcgctgc
     2341 acctggtagt ccttgagcac gtgcacgccg gccgcgggcg cggcgacgag cacgatcgtg
     2401 acggcggccg cgcccaggaa cccgagcacc gcgaagtggg tccacttggt gcccgcgacg
     2461 aacagcagcg cgcacgcgat cgtgatgtac acgaggcccg atcccaggtc gggctgggcg
     2521 atcaccagaa ttgccggcag cagcgcgagc aacatgatgc ggcgcgtggt gtcgcggtcc
     2581 gacattcggc gtgtgcggtc cacgagaaag ccggcgagcg ccaggatcag cagcagcttg
     2641 ccgagctcgg agggctggaa gttgaagaac gggaacgaga tccagcgctt cgagccgcgc
     2701 gtcgcgcccg cgagcgcgaa cacgatgacg atcgaggcga tcatcgtcgt gtagatcccg
     2761 atccgccatt ccctcagccg cgagtagtcg aagttcgcga tcacgaacat cagcaggaga
     2821 ccgacgcccg cgtacaggga ctgccgcagc acgtagtagg tcgggtcgcc cgggatgtcg
     2881 gtgttggtgg ccgtccagat cgtgtagatc gaggcgccga cgatccccag ggtcgccagg
     2941 agcagcagcg gatcctgccg gagcaggcgc aggccgggcc ccgggcccgg ctcgcggaag
     3001 tccctaacgg ttgtgccgag gcccgcctgc atcagctccc cgcctgcacc ctatagacgt
     3061 tggtggccgg gttcatcgcg tgcggctcgc acccttcacg accttgttct cctggctcct
     3121 gatcccaaac agcgcagcga ggatccggcg tgcatccgga gcagcggtct cggcgccgaa
     3181 gccgccctgc tcgtcggtga ccgcgaccac gtagcgcgga ttcgggtacg gcgcgagccc
     3241 gatgtaccac gactggtccg ggcgcccgag acccttctcc gcagtaccgg tcttgccggc
     3301 gaccttgatc gggaagtcct tgaacacgtc ggccgacgta ccgcccggct ggttcgcggc
     3361 catgaacagg ccgtcgagga tcgcctggcg gaactccggc cggaccgaga ccctgcggcg
     3421 ggtcggcgtg ccgaactcct ggatcgcgcg cccttcgctg tcctcgatcc tcatgccgag
     3481 gtgcgggcgg acgatgtagc cgccgttcgc gatcgaggcg taggcgaccg ccatctgcag
     3541 cggatccgcc tggaggtcgc cctggccgac cgcgaggttc acattgtcgc ccgccgacca
     3601 tggccgatcg gtcaacttct tcttgaacag ccggtcgcgc cactccgggc tcggcacgag
     3661 acccgactgc tcggccggta ggtcgatccc ggtcgagtgg ccgagcccga gctttcgtgc
     3721 ccagctctgg agcacgtgac cgttgccgac gttgttcagc tcggccccga gcttgtagaa
     3781 gaacacgtcg ctcgagacct tgatcgcatc acgcagcgcc agggtgccgt gtcccgtctt
     3841 gccggcgttg tagaaggttg tcggaccgag cttgaagaaa ccggggtcgt actgcggcgt
     3901 gtcgggcgtg atgaccccgc tctggagcgc ggccaccgac gtgatcagct tgaaggtgga
     3961 gcccgtcgga tagaggcctt ggatcgcgcg gttcgacagc ggcgatccgt tcgccgcgga
     4021 ggtgagcgcg tcgtagtcct tcttcttgat gaccttcgcg aagatgttcg ggtcgaacga
     4081 cggggagctt ccgagcgcgc gcacctcgcc ggtcttgatg tccatcacca cgaacgcccc
     4141 gcgccgtccg ttgagcgccg cttggccgat gcgctgcgtg ttgaagtcga gcgacagacg
     4201 cagctggtgg ccctgcaccg gattgcgctc ggcgagcgtg ccgcgcagcg tgcccgtcga
     4261 gtccacctgc acgcggtcag cgccgtcctg gccgcgcagg tagcggtcgt acgagtactc
     4321 gatccccgac tggcccacgc ggtcgccctg caccacgccg cgatagcgct gcttgtcctt
     4381 cagctcctgt gaggtgacct cgcccacggt tccgaacagg tgcgcgccga tcgagcggtg
     4441 tgggtagcgg cggaggaaga tgcgctcgac atcgacgccg gccagctgtt cctggtgctc
     4501 gagcacgtac gagaactgcg cctgagtcgc gtcctgctta acgatcgcgc gcgaaaacgg
     4561 cacggccttg agctgagttg ccacgttctt gcggatctcg cgccatggct ggccgacgac
     4621 cggcgacagg cgcttgtaga gcatctcgcg cgacttgcgg tccggcggga gcttgtcggg
     4681 ccgaacgacg atcgcccggc cggggcggtt gtcgacgagc acgttgccgt tgcggtcgac
     4741 gatcttgccg cgtggcgcgt cgacgcggat ctcgcgcacg cggttgttgt tcgcgtcggc
     4801 gaggtacttg tcgccggaca ggatctggag gtaccagagc cggaagaaga tcaccccgaa
     4861 catgaccagg gcaaagccgc ccaggatggc gacgcgaagc gccagctggg gggtgatcgc
     4921 cgggcggtgg tcggtgtccc ggtacacgcg ggctaccggc ctatctctag gccgcgcagg
     4981 ccgatcgggc cactctctcc agccaccgtg cgacgcctgc ggcggcgctc cagcggatcg
     5041 acgacaagca ccggcctcag cacccggcgc accaagatga agaacggcat cgcgatcagg
     5101 gtgttcagga agatcgtcag caacatcgag cgcagcgcga ggacgctgac ggacgagttc
     5161 gtctcgagca tgaaactgac gatcgcgatg ccgacgacgt agcccacgct ggcggccgcg
     5221 cccacgacga gcgggagcag gccgtggccc gagtcgcgaa gctcgcggta gctgcccacc
     5281 cagtagccga caagcgtgag cacgagcgag gagagcccga ggctctgccc gagcaggagg
     5341 tcgagcagca ggccggtggc aaagccggtc cccgcgccgg cgagcgcgcc cccgtagagc
     5401 gcgacggcgc ccacgagcag cggcaccagg tcggccgtgc cgcccagcac gtgtatgccg
     5461 gagaagcccg agatgtcgag cagcacggtg accagcgcaa gcaccccgag ccgcacgatc
     5521 gcgccaggcg tgacgatcac cgcgcgctca ccaccctcga gcggttcccg ctcgtaccgg
     5581 tgagcacctg cacgtagtcc atccgccgca ggtcggcgaa gggctggatg tgcacccgct
     5641 ggtagagctc gagctcgcct ggctcgacgc gcgtgacgcg cccgatcggg atgcccttcg
     5701 ggaacagcga ctcgagcttc gacgacgtga agcccgatgt cacgaccgtc tggcccttga
     5761 cgatgtcgcg gccgcgctcg atgtagtcga gcagcaggtc gttcggattg ccgacctgcg
     5821 gcgtgacgat gccgctcgcg cccagcggca cgacctcggc ggaaaccgcg ctcgagccgt
     5881 cggtgatcag cgtgacgcgg gcattgcccc cggtgacgga ggtgatcttg ccagcgaggc
     5941 cgttcccggt cacgaccggc tggttcatgc gaacgccgtc cgacgagcct ttgtcgatgt
     6001 ccaccgtgga gtaccagagc gtgggcgagc gggcgatcac gcgcgccgtc acgggcttgg
     6061 cgctcgccgg gtagccggcc ttcttcggca gccccagaag cgcccggaac tgctcgttgt
     6121 cgcgcgtcgc cacctggccc tgtgcggcct gagcgcggag ttgtgcattc tccgtccgca
     6181 gccgcttgtt ctcgcccttt gcgtgcacga cgtccccggt ccagccggca atgtcgcgga
     6241 acggcttaag cactttcgaa agcccggatt cgatgggcgc gaacgcggtc tgcgcgccgc
     6301 gctggattgc gtgcagggcg ccgccggtcg actcgccgaa gtacacggtc aggagcgcga
     6361 gcgagagtcc gaccaatacg gcgagggccg cccgccgcct gcggaccacc ttcttgtcgt
     6421 acacgggcgc gacctcccgc ggcgctcagt aacgccggcg gtttcgggaa ttccggttgc
     6481 tgcggtggat ggcctcgaac tcctccaggg agcggccgga tccgacagcg acgcaggtta
     6541 gcggtgactc cgccagatgg gcgggcatct gcgtctcgtc gcggaggcgc tcatcgaggc
     6601 cctggagcag ggagccgccg ccggcgagca tgatgccgcg gtccatgatg tccgcggcga
     6661 gctcgggcgg ggtgcggtcg agggtctcct tcacggcgtc gatgatcgct tgaagcggct
     6721 cctcgagcgc cattctcacc tcctcgctgg tgagaaccac ggtcttcggc aggccggaga
     6781 cgagatcgcg gccacggatc tccgcctgca cctcctcctc gagcgggaag gccgagccga
     6841 tctcgagctt cacctcctcg gcggtctgct gcccgatcat cagcttgtac tcgcgcttga
     6901 cgtagttgat gatcgcctcg tcgagctcgt cgccgccgat gcggatcgac tgcgagacga
     6961 cgatgccgcc gagcgagatc acggcgacct cgctggtccc gccgccgatg tccaccacca
     7021 tgttcccggt cggctcgccc accgggagcc cggcgccgat cgccgccgcc atcggctcct
     7081 cgatcaggta ggcctggcgc gcaccggccg agaggcaagc ctcctccacg gcgcgcttct
     7141 ccacgccggt cacgccggac ggcacgcaga ccacgacgcg cgggtgcgcc cagcggttct
     7201 ggtgcacctt ctggatgaag tggcggagca tctgctcggt cacgtcgaag tcggcgatca
     7261 cgccgtcctt cagcggccgg atggccgaaa tcgtgccggg cgtccggcca agcatgcgct
     7321 tcgcctcgat gccaacggca tgcacctcgc cggtccggtt gtcgaccgcc accacggacg
     7381 gctcagagag cacgatcccg cgcccacgca cgtaaacgag cgtgttcgct gtgcccaggt
     7441 cgaccgccat gtcgcggccc ccgaagccgg tcaggtacga gaaaaatccc attttgcgag
     7501 gaagcctaat tcgaccggtg gcggcacgcg cacttcgccc ctgcacgctc ctggccgagt
     7561 ccagtgggcc gaggcagctg gcttaccgcg atcccgtcgg aggacgagag tgtaacgact
     7621 cgcgccgggt cttccaggga cgccgccgta ccccgtttgg gtcacggcat cccgttgcgg
     7681 accacatccg gggcaagccg gaccagttcc gcgaccggca ggcccacgac gttccagaaa
     7741 tcgccgtcta tctcctccac tagcgccgag cccctcccct ggatcgcata ggcgcccgcg
     7801 cgctcgcacc actcaccgct atccaggtac cagcgcaggt cggccggctc gagttgcctg
     7861 aacgtgacct gagtgacggc atggccggtg cgctcgttgt cgcggtcgcg cagcacgatc
     7921 ccgctccaca cctggtgcgt gcggcctgag agcgtgcgta ggtagctctc agcctcgcgt
     7981 tcgtccgcgg gcttgccgta ccactggccg ccgagcacga cagcggtgtc ggcggccagc
     8041 acgcagtcgc cgtcgaccgc gcgcgccttg cggagcgcgt tctcgacgac gccctcgcgc
     8101 ggatccccgc cggagacctc ctctacgtct ggcacgctca cctcgaactc gatgccgagc
     8161 tgctcgagga tcgcccgccg ctgaggcgag cgcgaggcga ggatcaactt gggcgccatg
     8221 attcacgaac tgaagcactt aggggatgag tccgggacgt gggtgacttt ggttgcggat
     8281 agcgcaacca aagtcaccca cccctcctag ctcaccctca ttgccgctgt aagcgcacgc
     8341 ggcaccgagg cgcgcgcgga gacctacaac tccgggaaga acagctccac ctcgcgcgcg
     8401 gacgactccg gcccgtccga gccgtgcacc aggttcgtct gcacctcgag cgcgtagtcg
     8461 ccgcggatcg agccgggggc ggcctcgagc gggtttgtcg ccccgatcac ctgccgggcg
     8521 gccctgatcg cctcgtatcc ctcgagcacc atcgctacga gcggcccgcc ggtgatgaac
     8581 tccacgaggt cgccgaagaa cggcttctcg ctgtgctcgg cgtagtggcg ttcggcgagg
     8641 tcgcgctcga cttgcatgtg cttcagcgcg acgatgcgca accccttgcg ctcgaagcgg
     8701 gcgatcacct caccggtgag gccacgcgcg aaggcatcgg gtttcacgag gatcagagtc
     8761 cgctccattc agttccttct ctggtcgggg ggaaacagtc agcgagccgg acgctcgcca
     8821 cccgccggcg gacgttgtga cgccgctcgg gtgatcgtcg ggtcgtcccc gactcttgct
     8881 gccgccgtgg tctgccgccg cgggcgcagg cgctgccgct cgggcggagc ctggccgatc
     8941 tccgactcgc agtacgggca gatcttccag cgcgggtcga gcggcttacc gcaggtgcgg
     9001 cacggctcct tcagccgccg caggcagctc gggcagcgga ggaacgtgtt ctccacctcg
     9061 aagccgcagt acgggcagct cgggtccagc ttgtgcagcc gcgcttgagc cgcggcgatc
     9121 tcgagctcgc gctcgcgcac gtcgtcgagg tactccggcg gccgcacgat catgtagacg
     9181 atcgtcccga tgaacgggaa gaacagcgac gccagagcgg cgcagccgac gagcatcgga
     9241 tcgtcgatcc gccggcgggc gtccgcgtag gtccaccaga ccagcgccag ccagatcacg
     9301 accaagcaca ggacgatgat gctgactgcg cggttcaggc catcgttgtg gatcccgaag
     9361 atggcgacag gcatgcgcgc ggagtgtacc cgcgttcggg gactttccct gcaccttccc
     9421 gccccgcggc gtctatagga agaggcgccc gatggcgtaa ccgacgccga agaacaccag
     9481 aatcacgatc gccaccacga cggcgacgac gccgatcatc gtcagcaggc tggggaactc
     9541 gtcgttgctc acagcatcga ggctcgaagc gcggaacggt cgcggacgag gtccgcgacg
     9601 aggtaaatcg agcccgttgc gagcacggcg ccgcccgggc cggccagctc gcgcgcccgg
     9661 gagaccgcgg cctgggggtc ggccacgttc tcgacaggtg gcccgccgag cttctcggcg
     9721 agcgactcga gcgtggcggg cgagagcgag cgcgggttgg cggagcgcgt gaagatcacc
     9781 gagtcgaaca gtggcagcag cgggcgaagc atgccggccg cgtccttgtc gtcgagaatg
     9841 cctacgacgc ccaccagccg tcgcgcgccg acgacgtcgg gcagcgcatc ggcgagcgcc
     9901 tcgcttcccg acgggttgtg cgcgccgtcg tagatcgtga gcgggtcgtc ggcaaccttc
     9961 tgcatgcgcc cgggcacctg tgtctcagcg gcggcgcgcc tcactgcttc cgggtcgagc
    10021 tcgccgagga acgcctgtgc cgccgcctcg gcgagcgcga agttgcggcg ctggaagccg
    10081 ccgagcgcgg cgagcggcag atgcgtggtc gccggtgcgt gcacaagccg tgcgtgacgc
    10141 tcgcgcacga cgcgctcggc gacggcctcg gcgtcggcgt cgaggtcccc ggccacgagt
    10201 gtcgcctggt cgcgcaccac cgcgagcttc tcctcggcga tgtccttcac ggtcggaccc
    10261 agccagcgtg tgtgctcgag gccaacgttc gtgatcacct gcacgcgcga ggggatcaca
    10321 ttggtggcgt cgtagcggcc gccgaggccc gcttcgacca ccgcgacctc caccccgcgc
    10381 cgcgccagct cgtggtaggc cgccgcagtg agcgcctcga actgagtgac agtgtcgccc
    10441 gcttcctgcg tgcggttcac gagctcggcc gcgtgctgga cgcggctcac tgaagccgcg
    10501 aagtcggccc ccgacaccgg tcgctcgtcc acctcgatcc gctcggtgaa cgagacgagg
    10561 tgcggcgacg tgtagctgcc ggtccggatc ccatgccgct cgaggatcgc cgcgatcatc
    10621 cgcaccgtgg acgacttgcc gttcgtcccg accacgtgaa tcgaggcaaa gcgacgctgc
    10681 ggcatgccga gcacggtcat cagtttgtgc atgcggtcga gcccgaaccg catgccgaag
    10741 agctcgaggt cgagcaggta gctctccgcc tggcgctcgt ccatcccggt cagctgagct
    10801 cctcgagctc ggccttgagg cgcgcgagct tctgccgctc ggcctccacg acgtgctcgg
    10861 gcgccttcga cacgaagccg ctgttggcaa gcttgccctc cgcgcgcgcg atctccgact
    10921 gcagccgctt cacctcggcc tcgcgccggc gctcggcctc gcccgtgtcg atcgcgtcgc
    10981 tgcgcagcac ctggaccgat ccgcgcggaa tcgcgaccgt tgccaccacg tcgccgttct
    11041 cgtcgctccc cggctcgagc tcgaagcgcg cgagccgggc gacgtggtcg agcgcgccct
    11101 cgtatccggc ggccaggatc cggcccggga tgcgcgcgcc cgcgggtgca ccgacctcgt
    11161 cgcggtagcg gcgaagcgct gtgatcgcct cgatcactga cccgacctgt ccctcggcag
    11221 cctcgtcgaa gcgctcgggg tccaccttcg gccacggcga cgccgccagc agcccgcgct
    11281 cgcccggcag gaacgaccag atctcctcgg tcacgaacgg gatcagcggg tgggcgagct
    11341 tgagcacccg ctcgaggacg tgcaggagcg ttgccgacag cgccgtgcga tccgcctgct
    11401 cgtcgtagag ccgcggtttg acgagctcga ggtaccagtc gcacacctcg ccccagaaga
    11461 agtcgtagag atcgagtgcg gcgcgtgaca gctcgaacga ctcatagcgg ccgccgatgc
    11521 gctcggtcgc gcgctcgagc cgcgagagga tccagcggtc ttcggcggtc tcgggacgtg
    11581 gcgccggctc gacgtccggg tcgaccttga gcagcaccaa ccgcgacgcg ttccagagct
    11641 tgttggcgag gtcctggccc tgcttcacgc gcttctccga gtagcgcacg tcctgcgtgg
    11701 aagccatcgc gatcaggccg aagcgcacgg cgtccgcgcc gtactcgtcg atctcgtcaa
    11761 gcggatcgat gccggtcccg agcgacttcg acatgcggcg accgtcgggg gcctggatca
    11821 cgctcgtaat cggtacgtcg gtgaacggga tctcgcccgt gaactcgatc cccatcatga
    11881 tcatgcgcgc gacccagagg aagatgatgt cgcgcgccgt caccagcacg tcggtgggat
    11941 agaaggcgcg cagctcgggc gtgtccgccg gccagcgcag cgtggcgaac ggccacagcg
    12001 ccgacgagaa ccacgtgtcg agcacatccg ggtcgcggcg cggcgacccc ttgccgcacg
    12061 ggcactgctc gggctcctcc tcggccgcga tcatctcgcc gcactcgtcg cagtaccaga
    12121 ccgggatctg gtggccccac cagagctggc gggacacgca ccacggccgg atgttctcca
    12181 tccagctcag gtacacgccg gcccacgggt tctccgggtg gaagcggaca cggccctcct
    12241 tcaccacccg gatggccggc tcggcgagct tctccatgtc gcagaaccac tgcagagaga
    12301 tgagcggctc gatccgcatt ccgctgcggt gcgagtgcgg cacgtcgtgc tcgtacggct
    12361 cggtgccggc gatcgcgccc tcggcctcga gcgcggccac gacggcgtcg cgcgcgtcga
    12421 ggaccttcag gccggcgaac cgctcgcccg cggcctcggt catcaggccg tcctcgccga
    12481 tgacggtgac ctcgtccagc ccgtgcgtgc ggccgatctc gaagtcgttc gggtcgtgcc
    12541 ccggcgtgat cttcagcgcg ccggtgccga attccggctt gacgtacggg tcggcgatga
    12601 tcttcaggcg gcggccgacg agcggcagga tcgcggtctc gccgatcagg cgggtgtagc
    12661 gatcgtcgtc cgggtgcacc gcgagcgcgg tgtcggcgag catcgtctcc gggcggacgg
    12721 tggcgatcgt gaccgacccc cggccgctct ccagcgggta gtcgatgtag tagagggtgt
    12781 cggtgacctc gcggtcttcg acctcgaggt cggagatcgc cgagcgcgac ccggggtccc
    12841 agttgaccat gcggttgtcg cggtagatca ggcccttgtc gtacaggtcg acgaagacct
    12901 tctggaccgc gtgcgcgtac ggcgggtcga ggacaggctg tactcgatcg actacccgct
    12961 cgagtcgggc tccggatcga taacgatcgc caccgtgcgg ccggagacg
//
LOCUS       DASVXS010000190        14740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_803891, whole genome shotgun sequence.
ACCESSION   DASVXS010000190 DASVXS010000000
VERSION     DASVXS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14740
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803891"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF752_10470"
     CDS             <1..381
                     /locus_tag="VF752_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX8856013.1"
                     /translation="IAEMRASIGADAARLCARRAPADTRETLTHLAAPRKDASYEEQL
                     VAYEELWKSIVDGSGNLGYRLAFNSLVGARHGHGVDSRIYADEVGDVRAWRALAAAVT
                     AGDEPRAERTARQLLDRTLEAVSW"
     gene            378..1235
                     /locus_tag="VF752_10475"
     CDS             378..1235
                     /locus_tag="VF752_10475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8856014.1"
                     /translation="MIEILYYAIPFFILLLIVEWLSFRQVRDDAELVGFEARDTRTSL
                     TMGLGNVVINVGWKLVVVAIFAGVYELTPLRLDPHDWWVWVLLFFADDVSYYWFHRVS
                     HESRVFWASHVVHHSSQHYNLSTALRQTWVPMTYFPFWLWMPLVGFPVWMVLLAQSWS
                     LIYQFWIHTERVRRLPRVFEAVLNTPSHHRVHHGSNEVYLDKNYGGILIVWDRLFGTF
                     EPEGERVRYGLTKNIHTFKPVRVAFHEYIALWHDLRAVRDWRTRVVLVFKGPGWRPEL
                     EEVSEGIRG"
     gene            1272..1928
                     /gene="msrA"
                     /locus_tag="VF752_10480"
     CDS             1272..1928
                     /gene="msrA"
                     /locus_tag="VF752_10480"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8856015.1"
                     /translation="MLFSRHKTEMISADRALEGRDERMPVPSRHYVNGNPLEPPFPEG
                     IETAVFAMGCFWGAERLFWETPGVYTTAVGYAGGYTPNPTYEEVCSAHTGHTEAVLVA
                     YDPKQVSYDELLRVFWEGHDPTQGMRQGNDVGTQYRSAIYTTTEAQAEAARASRDRYQ
                     EALNASGHGQITTEIAPAGPFYYAEDYHQQYLAKNPNGYCGIGGTGVSCPVGVGTTAS
                     "
     gene            complement(1939..3078)
                     /locus_tag="VF752_10485"
     CDS             complement(1939..3078)
                     /locus_tag="VF752_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856016.1"
                     /translation="MKAHRLALAALAVFVLGLCGVAQARTVKISEFRLPDSRLDALRS
                     DWVDGLLTRYPQGSWAPMRQELTNRDLRLMGLPPKRVLASHRYRTPTAVYPSGKMVKL
                     AAGKRSKQGGSRTPAADQPGLVTFAGTGFFGIRPGAWLLLINGDSVGWCSMAHVYGSP
                     GAYQISTAGHCGKVGDTATVIGAVGNRTVNGVPVPVLLDFGKFSKSTGDAGIGKDWAL
                     ISVLPQYQSLVTPTMAFWGGPRGMFTSTGEIVSVNLLGKQPTVGVNPNPTLVQQILHY
                     GHGAGIGAGGTPRSGTSITWRPNYFTFFGAITPGDSGSGSNTLGGDGVGDEREAAGIN
                     THIYVDPSLKTGVGMMAGTRATLVTAPLADGQLLPYPAPTPVPLP"
     gene            3158..4096
                     /gene="ligD"
                     /locus_tag="VF752_10490"
     CDS             3158..4096
                     /gene="ligD"
                     /locus_tag="VF752_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8856017.1"
                     /translation="MPIGLMLATLSDRREFGDDWLLERKFDGERCVARKDGGGVRLES
                     RTGKDLTGTYPEVRAALEAQRARELLVDGEVVAFDGDQTSFSRLQQRLGDRRPSPEKV
                     EQFPVVYCVFDLLEIDGEDLRGRPLMERRARLADEIRPKPGLQLSEAWLGDSQRRFAE
                     ACRSGWEGLIAKRGDAPYVAARSRDWLKLKCLWEQEFVIGGYTDPAGSRTDFGALLVG
                     YYDDTDLRYAGKVGTGYNATTLRELGARLRKVEVPESPFADARPIPRGTHWTRPELVA
                     QIGFAEWTNDGRLRQPRFLGLRDDKRARDVVRESAR"
     gene            complement(4117..5307)
                     /locus_tag="VF752_10495"
     CDS             complement(4117..5307)
                     /locus_tag="VF752_10495"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX8856018.1"
                     /translation="MGTATDSTAIRPFHIEVPEEQLAELRRRIEATRWPSRELVDDRS
                     QGVQLATMQALARYWTTDYDWRKCEAKLNALGQFMTEIDGVDIHFIHVRSPHEDALPL
                     IMTHGWPGSVVELLETVGPLTDPTAHGGDAEDAFHLVLPSIPGYGFGGQPAEVGWDAA
                     RIGQAWAELMKRLGYDRYVAQGGDRGAVVTDAMARQAPEGLLGVHINVLIQLDRLAEL
                     PAESEQERAAHAAVKTFTTDGIGYLLEMATRPQTIGYALLDSPIALAAWMFDHDTDGY
                     YKFAGAFVDGKPTGNLTRDHILDDVTLYWLTGTGVSAARDYWDDGRFRARLADEPLPP
                     AAVPVGFTTFPGEVWAAPRSWVEVIYPDLAYFDEVDKGGHFAAWEEPELFSAEVRAAF
                     RSLR"
     gene            complement(5482..5961)
                     /locus_tag="VF752_10500"
     CDS             complement(5482..5961)
                     /locus_tag="VF752_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8856019.1"
                     /translation="MSDEVEITNHRVDATQNESLIGIARMGADAFGYTAELRIDRGLA
                     QLLRLRVSQLNNCSYCLNLHYEAARDAGIPRAKIDTLTAWWETDLHSEAEQAALRYAE
                     ALTRAPDTDAGQAFQRFHDGLAEHFDAEKILEIIGVVVNMNVWTRLKLAEGAKPRLG"
     gene            6212..6625
                     /locus_tag="VF752_10505"
     CDS             6212..6625
                     /locus_tag="VF752_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX8856020.1"
                     /translation="MVTATDVAELLATHPKPSEVDREALARCIADCFDCARSCTACAD
                     ADLAEEDVSELRRCIRLCLDCADVCEATGRVMSRQTEYEPETSRAQVESCRQSCSACA
                     EECERHAHHHEHCRLCAETCRRCEDACARLLDAIG"
     gene            complement(6719..8215)
                     /locus_tag="VF752_10510"
     CDS             complement(6719..8215)
                     /locus_tag="VF752_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8856021.1"
                     /translation="MQGTGITRRRLVGGATATALAAAVPSQVARAATKGTQRPARSAD
                     VIVVGAGLAGLAAARTVAAQGKSVLVLEARDRVGGRVLNHDLGNGDYSELGAMFTGPT
                     QTRIQQLATDVGVGLYKTYNTGNNVSYVDGRRMAYPANTPLGIVPPDPLVAADAAAVV
                     TLLDQLSQDVPVDKPWESSKADEYDRQTLDTWIREHSSGNPDFMSIVQAATNAIFGAE
                     ARDISLLYTLFYIAASGDETHAGTFERNFNTADGAQESRFKGGAYQIPIKVAAQLGSS
                     VVLSAPVRRVSQTSDGVSVVSDASTFAGKRVIVAMPPALAGRIVYDPIMPPLRDQLTQ
                     RMPQGTLMKFEAIYDKPWWRDKGYTGQAVSGQGAIRVTFDITPEDGSPGILMGFIGGH
                     EARTWEQRSPDDLKKECLNNLATLFGEDARAVKDTVSFNWSTEEWSRGCPVAIVGPGT
                     YSDFGTALRAPVGRIHWAGTETATYWNGYMDGAVRSGERAAAEVLAAL"
     gene            complement(8273..9211)
                     /locus_tag="VF752_10515"
     CDS             complement(8273..9211)
                     /locus_tag="VF752_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX8856022.1"
                     /translation="MKTVALGAYILDVLVRPVTEIPAGQDAALVEEIRLAPAGAAGGT
                     AVTLAKLGADVHAAGAVGRDTLGDVLIEMLEQYGVHTDRLARRDDLQTSSSVIPIRPT
                     GERPALHVIGANLSYTPGDAPLDLIAAADFLHVGGPELLGETAELVLKAAKDAGATTS
                     VDVLADGWPELLDMIAPALPYVDWITPNDEQAKRLTGEADIESAAAVLIELGAGGVIV
                     TCGADGVLIVTPDGQTRVPAFEIEVIDTSGCGDAFVAGFLRGLSLGERPADAARLGSA
                     TAALVAQGTGSDHGEFDLQRVQAFASSTPMREPAQG"
     gene            complement(9208..9882)
                     /locus_tag="VF752_10520"
     CDS             complement(9208..9882)
                     /locus_tag="VF752_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8856023.1"
                     /translation="MGASGARKLASERAAVAAAARRLADAGLVERTAGNVSARAGELV
                     AVTPTGAQLGSLEPEDVTVVDLSGAVVDGRFEPTSETALHLAVYESTQAGAVAHTHAP
                     MATAVGCVVDELPLVHYEMLALGGPVRVAPYATFGTQQLADSVAAALEGRAAALMQNH
                     GAVTIGPDAGTAVELTLLLEWAATLFWRARQIGEPRSLGEQDVKDFFDTVARRGYGVT
                     KEVDEQ"
     gene            9924..10949
                     /gene="mgrA"
                     /locus_tag="VF752_10525"
     CDS             9924..10949
                     /gene="mgrA"
                     /locus_tag="VF752_10525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX8856024.1"
                     /translation="MTYIAREDRYERMLYRRCGRSGIDLPAVSFGLWNNFGDDRPIET
                     QRAILRRAFDLGVTHFDLANNYGPPYGSAELNFGRIMREDLRPYRDELIISTKAGYDM
                     WPGPYGEWGSRKYLLASLDQSLQRMGLDYVDIFYSHRHDPNTPLEETMSALETAVKQG
                     KALYAGISSYSGARTKDAFEILHNRGIRLLIHQPSYSLLNRWIEDDLLDVLGEEGIGC
                     IVFSPLAQGMLTSKYLDGVPEGSRMAEGRFLTTDMLTEEALGRIRALNEIAARRGQSL
                     AQMAIAWTLRDPRVTSALIGASSVEQLEENIAALDNLDFDDDELHDIDRYAVDSGINI
                     WAASSER"
     gene            complement(10936..12123)
                     /locus_tag="VF752_10530"
     CDS             complement(10936..12123)
                     /locus_tag="VF752_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8856025.1"
                     /translation="MSGVDATSFLVIVAVAALAGVIATFAASRVLVPVVVLELVLGIV
                     VGPQVADIAHVDEFTDFFGNLGLGMLFFFAGYEIDFERIRGRPLQLGARGWALSLVLA
                     YGIGAALEGAGLVISFLYTGSALATTAMGTLIPILRDSGDLHTRFGTYLLSAGAVGEF
                     GPILLITLVLSTSHPVHEAVILVLFVALAVLTGVFAVRSAGRGWGVLERTLESSSQLA
                     VRLAVVLVFALVALASELGLDLLLGGFVAGLITRQALRGQEVHLFESKLTAVGYGFLI
                     PFFFVTSGMKFDLDGLTSSSSALAKLPLFLALFLVVRGLPALLIYRRDLDAPERSALA
                     LFTATELPLVVAVTTLATETGHMRSTTAAALVGAAILSSLIYPLMALKVRRATAPAAV
                     SAR"
     gene            12222..12743
                     /locus_tag="VF752_10535"
     CDS             12222..12743
                     /locus_tag="VF752_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856026.1"
                     /translation="MRKARIAGTTVAVAAVAMAVAGYATSVADSGGAGAAAARPKPTV
                     ALRTTSLGKILVDGKTGRTLYEFGKDRRNKSNCSGSCAAIWPPLTVSGKLLAGKGVSA
                     SKLTTIKRADGKRQAVYAGHPLYRFAADSKAGQTNGEGVNGFGGLWYAMNARGSLVKG
                     PPPSQPMNNPYGY"
     gene            12834..14009
                     /locus_tag="VF752_10540"
     CDS             12834..14009
                     /locus_tag="VF752_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8856027.1"
                     /translation="MRAVVCRNTELEVVDRPEPVPGRGQVRIEVLRCGICGSDLHARH
                     GSDDWADMAARVGYDRFARSNEPVVFGHEFCGEVVEHGPQCRRGSPTGTHVVALPLLR
                     GTHGVDTIGLSVHAPGGYAEQLLVEESLMMPVPNGLTPDAAAVTEPMAVAWHAVRRGE
                     VGKRTVAIVIGCGPIGLAVILVLKAKGVRTVVASDYSPGRRALASACGADIVVDPGHE
                     SPFAAAAGRHLKDLPSALELVVGTREKLERLPVGWWHAWRLAEALGATPKHPVIFECV
                     GVPGVIESIIDGAPLLSRVVVVGVVVGVDHITPAMAINKEIDLRFAFAYSPLEFRDAL
                     HMLAEGDIDPAPLITGTVGLEGVDAAFAALGSPERHAKILIDPRSSAAEPVASAPVA"
     gene            complement(14006..>14740)
                     /locus_tag="VF752_10545"
     CDS             complement(14006..>14740)
                     /locus_tag="VF752_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8856028.1"
                     /translation="VMPTFDAYYALERMCEDERKYSWLVCNWKIAVRELDVSGSQHNP
                     HLREKRLGRFVGSVLFGKPNLVRYFLNGCPDFVTAGSVPELAQRMAEVTNDDALDADL
                     LELEIARYDDNLGRGKSLWNDDQLRRIAQLRNWRGDRLRTARFARIVDEKAMPLIAIR
                     LQPMARKSLGGIQTDLECRVLTADGEPVEGLYAVGEAAGFGGGGMHGRRSLEGTFLGG
                     CVFTGRLAAKGIVADAGTRVTAHHAN"
BASE COUNT         2301 a         5133 c         5062 g         2244 t
ORIGIN      
        1 atcgccgaga tgcgcgcctc gattggcgcg gacgccgcgc ggctatgcgc gcggcgcgca
       61 ccggccgaca cgcgcgagac gctcacccac ctggctgccc cgcgtaagga cgcgagctac
      121 gaggagcagc tcgtggccta cgaggagctg tggaagtcga tcgtcgacgg gagcggcaac
      181 ctcggatacc ggctggcgtt caacagcctc gtgggcgcgc ggcacggcca tggcgtcgac
      241 tcacgcatct acgcggacga ggtcggcgat gtgcgcgcgt ggcgcgcgct cgccgcggcg
      301 gtgacggccg gcgacgagcc gcgggcagag cgcaccgctc gtcagctgct cgatcgcacg
      361 ctcgaggcgg tctcctggtg atcgagatcc tctactacgc aattccgttc ttcatcctcc
      421 tgctgatcgt cgagtggctg tcgttcaggc aggttcgcga cgacgccgaa ctcgtcggct
      481 tcgaggcgcg cgacactcgc acgagcctca cgatggggct gggcaacgtg gtcatcaacg
      541 tcggctggaa gctcgtggtc gtcgcgatct tcgccggcgt ctacgagctc acgccgctgc
      601 gcctcgaccc gcacgactgg tgggtctggg tgctgctctt cttcgccgac gacgtctcgt
      661 actactggtt ccaccgcgtg tcgcacgaga gccgcgtgtt ctgggcgagc cacgtggtgc
      721 accacagctc gcagcactac aacctctcga ccgcgcttcg ccagacctgg gtgccgatga
      781 cctacttccc gttctggctg tggatgccgc tcgtgggctt cccggtctgg atggttctgc
      841 tcgcgcagag ctggagcctc atctaccagt tctggatcca caccgagcgc gtgcgccggc
      901 tgccgcgcgt gttcgaggcg gtcctcaaca cgccgagcca ccaccgcgtg caccacggct
      961 cgaacgaggt ctacctcgac aagaactacg gcggcatcct gatcgtctgg gaccgcctgt
     1021 tcggcacctt cgagcccgag ggcgagcggg tgcgctacgg cctcacgaag aacatccata
     1081 ccttcaagcc ggtgcgcgtg gcgttccacg agtacatcgc gctgtggcac gacctgcgcg
     1141 ccgtgcgcga ctggcgcaca cgggtcgtgc tcgtgttcaa ggggccgggc tggcgacccg
     1201 agctcgaaga ggtctcggag ggcatccgag gctgacggtc gggcttgtcg gcgagggcta
     1261 acctggaggt gatgcttttc tcacgacaca agaccgagat gatctccgcc gaccgcgccc
     1321 tcgagggccg cgacgagcgg atgccagtgc cgtcgcggca ctacgtgaac ggcaacccgc
     1381 tcgagccgcc gttccccgag gggatcgaga ccgccgtgtt cgccatgggc tgcttctggg
     1441 gagccgagcg cctcttctgg gagacgcccg gtgtctacac caccgccgtc ggctacgcgg
     1501 gcggctacac gccgaacccc acctacgagg aggtgtgcag cgcgcacacc ggacacaccg
     1561 aggctgtgct cgtcgcctat gaccccaagc aggtgagcta cgacgagctg ctgcgcgtct
     1621 tctgggaggg ccacgacccg actcagggca tgcgccaggg caacgacgtg ggcacccagt
     1681 accgctccgc gatctacacg acgaccgagg ctcaggccga ggccgcgcgc gcgtcgcgcg
     1741 accgctacca ggaggcgctg aacgcgtcgg gccacggcca gatcaccacg gagatcgcgc
     1801 cggccgggcc gttctactac gccgaggact accaccagca gtacctcgcg aagaacccga
     1861 acggctactg cggcatcggc ggcacgggcg tttcctgccc ggtcggggtc ggcacgaccg
     1921 cgagctagcg ccgccgcgtt acggaagcgg aaccggggtg ggagccggat agggcaagag
     1981 ctgcccgtcg gccagcggcg cggtcaccag agtcgcgcgc gtccccgcca tcattccaac
     2041 gcctgtcttc aacgacgggt ccacgtagat gtgcgtgttg atcccggcgg cctcgcgctc
     2101 gtcgcccacg ccgtcaccgc ccagcgtgtt cgagcccgag cccgagtcgc ccggggtgat
     2161 cgcgccgaag aacgtgaagt agttcggtct ccacgtgatc gacgtgcccg aacgcggcgt
     2221 cccgccggca ccgatcccgg cgccatgccc gtagtggagg atctgctgga cgagcgtggg
     2281 attcgggttc acgcccacgg tgggctgctt gccgagcagg ttcacgctca caatctcgcc
     2341 agtggacgtg aacatgccgc gcggcccgcc ccagaacgcc atcgtcggcg tgacgaggct
     2401 ctggtactgc ggcagcacgg agatgagggc ccagtccttg ccgattcccg catcgccggt
     2461 gctcttcgag aacttgccga agtcgagcag cacgggcacc ggtacgccgt tgacggtccg
     2521 gttgcccacc gccccgatca cggtcgcggt gtcgccgacc ttgccgcagt gacccgcggt
     2581 gctgatctgg tacgcgcccg gcgagccgta cacgtgcgcc atcgagcacc agcccacgct
     2641 gtctccgttg atcagcagca gccacgcgcc cggcctgatg ccgaagaagc cggttcccgc
     2701 gaaggtcacg aggcccggtt gatcagctgc aggggttcgc gagccgccct gcttcgagcg
     2761 tttgccggcg gccagcttca ccatcttccc gctcggatag acggcggtcg gcgtccggta
     2821 gcggtgtgag gccagaacgc gcttcggcgg aagtcccatc agccgcaggt cgcggttcgt
     2881 gagctcctga cgcatcgggg cccacgatcc ctgcggatat cgcgtaagca ggccgtcgac
     2941 ccagtcgctg cgcagcgcat ccagccggct gtccggaagc ctgaactcgg agatcttgac
     3001 cgtacgcgcc tgcgcgacgc cgcagagccc gagcacgaac acagccagcg cagccagcgc
     3061 gagccgatga gccttcatga gtggcacccc ctgccagttg aacgcggacc cggtataccg
     3121 aaccagccgc tcccctgcgc cggtaggctc gctcctagtg ccgatcgggc tgatgctggc
     3181 caccctgagc gaccgtcgcg aattcggcga cgactggctg ctcgagcgca agttcgacgg
     3241 agagcgctgc gtggcccgca aggacggcgg cggcgtgcgg ctcgagtcgc gcaccggaaa
     3301 ggacctcacc ggcacgtatc cggaggttcg cgccgcgctc gaggcgcagc gagcgcggga
     3361 gctgctcgtc gatggagaag tggtggcatt cgacggcgac cagacgtcgt tcagccgtct
     3421 ccagcaacgg ctcggcgacc ggcggccctc tcccgagaag gtggagcagt tccccgtcgt
     3481 ctattgcgtg ttcgacctgc tcgagatcga cggcgaggac ctgcggggcc gcccgctaat
     3541 ggagcgccgc gcccgcctgg ccgacgagat ccggccgaag ccgggactgc agctgagcga
     3601 ggcctggctt ggtgactcgc agcgccggtt cgccgaggct tgccgctcgg gttgggaggg
     3661 actcatcgcc aagcgcggcg acgcacctta cgtcgccgcc cgctcgcgcg actggctgaa
     3721 gctgaagtgc ctctgggagc aggagttcgt gatcggcggt tacacggacc ccgccggcag
     3781 ccgcaccgac ttcggcgcgc tgctcgtcgg ctactacgac gacactgacc tgaggtacgc
     3841 gggcaaggtc ggcaccggct acaacgccac gacgctgcgc gagctcggcg cacggctccg
     3901 caaggtcgag gtaccggaat cgcccttcgc cgacgcgcgc ccgataccgc gcggcacgca
     3961 ctggacgcgc cccgagctgg tcgcccagat cggcttcgca gagtggacga acgacggccg
     4021 cctgcggcag ccccgcttcc tcggactgcg cgacgacaag cgcgcccgcg acgtcgtccg
     4081 cgagagcgcc cgctgagcgc caccgccgtc gcgttgttag cgcagtgagc ggaatgcggc
     4141 ccgcacctcg gcggagaaca gctccggctc ctcccaggcg gcgaagtggc cgcccttgtc
     4201 gacctcgtcg aagtaggcca ggtcggggta gatcacctcg acccagctgc gcggggcagc
     4261 ccagacctcg ccggggaacg tggtgaagcc gaccggcacg gccgctggcg gaaggggctc
     4321 atcggccaag cgggcccgga agcgcccgtc atcccagtag tcccgggccg ctgagacccc
     4381 ggtgcccgtc agccagtaca gcgtgacgtc gtcgaggatg tggtcccggg tgaggttgcc
     4441 ggtcggcttg ccgtccacga aggcgccggc gaacttgtag tagccgtccg tgtcgtggtc
     4501 gaacatccac gccgccaggg cgatgggtga gtcgagcagc gcgtatccga tcgtctgcgg
     4561 ccgcgtggcc atctccagca agtagccgat gccgtccgtc gtgaacgtct tgaccgcggc
     4621 gtgtgccgcg cgttcctgct cggactccgc cggcagctcc gcgaggcgat cgagctggat
     4681 gagcacgttg atgtggacgc cgagcaggcc ctcgggcgcc tgccgggcca tcgcgtcggt
     4741 gacgacggct ccccggtcgc cgccctgggc gacgtagcgg tcgtagccga gccgcttcat
     4801 caactccgcc caggcttgcc caatgcgagc cgcgtcccag ccgacctcgg ctggctgacc
     4861 cccgaagccg tagccgggta tcgacgggag aaccaggtgg aacgcgtcct cggcgtcgcc
     4921 accgtgcgcg gtcgggtcgg tcagcgggcc gacggtctcg agcagctcga cgaccgagcc
     4981 gggccagccg tgcgtcatga tcagcggcaa cgcgtcctcg tgcggcgacc gcacgtggat
     5041 gaagtggatg tccaccccgt cgatctccgt catgaactgc cccagcgcgt tcagtttcgc
     5101 ctcgcacttg cgccagtcgt aatcggtcgt ccagtagcgc gcgagcgcct gcatcgtcgc
     5161 cagttgcacg ccctgcgagc ggtcgtcgac caactcgcgg gacggccagc gcgtcgcttc
     5221 gatgcggcgg cgcagctcgg cgagctgctc ctcaggaacc tcgatgtgga acgggcggat
     5281 cgctgtcgag tcggtggcgg tacccatgac aatctccgtg ctgtaggggc gggaccctcg
     5341 ccacgctacc cctgcagatg gggcgtagca acgaatggca ccagatcgaa acagggccgc
     5401 tccgcactcg tcgcgcctcg cgtcaaagca tgcgcgggcg cggcgccggt ggtgtccggc
     5461 ggcgtcaagc acgctcgcag ctcagccgag ccgcggcttc gcgccctcgg cgagctttag
     5521 tcgcgtccac acgttcatgt tcacaacgac gccgatgatc tcgaggatct tctcggcgtc
     5581 gaagtgctcc gcgagcccgt cgtggaagcg ctggaaggcc tggccggcat ccgtatcggg
     5641 ggcccgggtg agcgcttcgg cgtagcggag ggcggcctgc tctgcctcgg agtggaggtc
     5701 ggtctcccac cacgcggtca gcgtgtcgat cttcgcgcgc gggatcccag cgtcacgggc
     5761 ggcctcgtag tggaggttga ggcagtagct gcagttgttg agttgcgaaa cccgcagtcg
     5821 caggagctgc gcgaggccgc ggtcgatgcg cagctccgct gtgtacccga acgcgtccgc
     5881 gcccattcgc gctatgccga tcagggactc gttctgggtc gcgtcgacgc ggtggttcgt
     5941 gatctcgacc tcgtcgctca ttcgtcttcc cttcggtcgt catcggtctt cgtgatccca
     6001 ctttccggcg gcgcccctaa acgctttgtc gcaatctgct cgcggaagcc cgcgtcctcg
     6061 cgctggacct gttggacgcc ctcgatcacc ctttcgcggc cgccggcggg atcatcccag
     6121 cggacagcgc ttccgtcttc gtggagcacg agcaggcggg gtacgatggg ccacggcggc
     6181 cctggcgggc aaacccgacg gtggaggagt catggtcacc gcaaccgacg tcgccgagct
     6241 actcgccacc cacccgaagc cgagcgaggt ggatcgcgag gcactcgccc gctgcatcgc
     6301 cgactgcttt gactgcgccc gcagctgcac ggcgtgcgcg gacgccgatc tcgccgagga
     6361 ggacgtctcc gagctgcgga gatgcatccg cctgtgcctc gactgcgccg atgtctgcga
     6421 ggccacaggg cgtgtgatga gccgtcagac cgagtacgag ccggaaacct cgagagcgca
     6481 ggtcgagagc tgccggcagt catgttcggc atgcgccgag gagtgcgagc gccacgccca
     6541 tcatcacgag cactgccgcc tctgcgccga aacctgtcgg cgttgcgagg acgcgtgtgc
     6601 ccgcctgctc gacgcgattg gataagcaca cacacgacga cctgactagc tgatcacggc
     6661 gaggccaagc accggctccc agagtgcgtc gcctacctcg cgctcgactg agcgccggct
     6721 acagcgcggc gagcacctcc gcagccgcgc gctcacccga gcgcaccgcg ccgtccatgt
     6781 agccgttcca gtaggtggcc gtctcggttc cggcccagtg gatgcgcccg accggcgccc
     6841 gcagcgccgt gccgaagtcg ctgtaggtgc cggggcccac gatcgccacc gggcagccgc
     6901 ggctccactc ctcggtgctc cagttgaagc tcaccgtgtc cttcacggcc cgcgcgtcct
     6961 caccgaacag cgtggcgagg ttgttgaggc actccttctt caggtcgtcg ggggagcgct
     7021 gctcccaggt gcgcgcctcg tggccgccga tgaatcccat gaggatcccc gggctgccat
     7081 cctcgggcgt gatgtcgaac gtgacacgga tcgcgccctg cccgctcacg gcctggcccg
     7141 tgtagccctt gtcgcgccac cacggcttgt cgtagatcgc ctcgaacttc atgagcgtgc
     7201 cctgaggcat ccgctgcgtg agctggtcgc gcagcggcgg catgatcggg tcgtagacga
     7261 tgcggccggc gagggcgggc ggcatcgcga cgatcacacg cttgcctgcg aacgtgctgg
     7321 cgtcggagac gacgctcacc ccgtcgctcg tctgggagac gcgccgcacc ggggcgctca
     7381 gcacgacgct gctgccgagc tgggccgcca ccttgatcgg gatctggtac gcgccgccct
     7441 tgaagcgcga ttcctgcgcg ccgtcggccg tgttgaagtt gcgctcgaac gtgcccgcgt
     7501 gcgtctcgtc gcccgacgcc gcgatgtaga agagcgtgta gagcagcgag atgtcgcgcg
     7561 cctcggcgcc gaagatggcg ttggtcgcgg cctgcacgat cgacatgaag tcggggttcc
     7621 cgctcgagtg ctcacggatc caggtgtcga gcgtctggcg gtcgtattcg tcggccttcg
     7681 acgactccca cggcttgtcg acgggcacgt cctgggacag ctggtcgagc agcgtcacga
     7741 cggccgctgc gtcggccgcc accagcgggt cgggaggcac gatgccgagg ggggtgttcg
     7801 ccgggtatgc catgcggcgg ccgtcgacat acgagacgtt gttgccggtg ttgtaggtct
     7861 tgtagaggcc gacgcccacg tcggtcgcca gctgctggat acgggtctgc gtcggcccgg
     7921 tgaacatggc gccgagttcg ctgtagtcgc cgttgccgag gtcgtgattg agcacgcgcc
     7981 caccgactcg gtcacgcgcc tcgagcacga gcaccgattt gccctgggcg gccaccgtgc
     8041 gcgccgcggc gaggccggcg agtcccgcac ccaccacgat cacgtccgcg ctgcgtgccg
     8101 gccgctgcgt tcccttggtt gccgcgcgcg ccacctgcga cggcacagcc gcggcgagcg
     8161 ccgtcgccgt ggcgccgcca acgagacggc gccgggtgat cccggtcccc tgcatatgag
     8221 aaacgcctcc tgtacccgct gccttgccgg cgcgaatctt gccgggcgcc ggctatccct
     8281 gcgccggttc cctcatcggt gtggaggagg cgaacgcctg cacgcgctgc aaatcgaact
     8341 cgccgtggtc gctgccggtt ccctgcgcca caagcgctgc ggtggccgag ccgagacgcg
     8401 cggcatcggc cggccgctcg ccaagcgaga gcccccttag gaagcccgcc acgaacgcgt
     8461 caccgcaccc cgacgtgtcg atcacctcga tctcgaaggc cgggacacgc gtctgcccat
     8521 cgggcgtgac gatcaacacg ccatcggcgc cgcaggtgac gatcacgccg cctgcgccga
     8581 gctcgatcag caccgcggcc gccgactcga tgtcggcctc gccggtcagc cgcttcgcct
     8641 gctcgtcgtt cggcgtgatc cagtcgacgt acggcagcgc cggagcgatc atgtcgagca
     8701 actcgggcca gccgtccgcg agcacgtcga ccgaggtggt ggcgccggcg tccttggccg
     8761 ccttgagcac gagctccgcg gtctcgccca ggagctcggg gccacccacg tgcaggaagt
     8821 cggctgcggc gatcaggtcg agcggcgcgt cgccgggggt gtagctcagg ttcgcgccga
     8881 tcacgtgcag cgccggacgc tcgccggttg ggcgtatcgg gatgacgctc gacgacgtct
     8941 gcaggtcgtc gcggcgcgcg agccggtcgg tgtgcacgcc gtactgctcg agcatttcga
     9001 tcagcacgtc gccgagcgtg tcgcggccga ccgcgcccgc ggcgtgcacg tccgctccga
     9061 gcttcgcgag cgtgaccgcc gtgccgcccg cggcgcctgc cggggcgagc ctgatctcct
     9121 ccaccagcgc cgcgtcctga ccggccggga tctccgtgac cgggcgcacg agcacgtcga
     9181 ggatgtacgc gccgagcgcg actgttttca ctgctcgtcc acctccttgg tcacgccgta
     9241 accacgccgg gcgaccgtgt cgaagaagtc cttcacgtct tgctcgccga gcgagcgcgg
     9301 ctcgccgatc tggcgagcgc gccagaacag tgtggccgcc cactcgagca gcagcgtcag
     9361 ctcgaccgcg gtgcccgcgt cggggccgat cgtgaccgcg ccatggttct gcatgagcgc
     9421 cgctgcgcgg ccctcgagcg ccgccgcgac cgagtcggcc agctgctgcg tgccgaacgt
     9481 cgcgtacggc gcaacgcgaa cggggccgcc cagcgcgagc atctcgtagt gcacgagcgg
     9541 gagctcgtcg accacgcagc ccacggctgt cgccatcggc gcgtgggtat gcgcgacggc
     9601 gcccgcctgc gtggactcgt agaccgccag gtgaagcgcc gtctctgagg tcggctcgaa
     9661 gcgcccgtcg accactgcgc ccgacaggtc cacgaccgtc acatcctccg gctcgagcga
     9721 gccgagctgc gcgcccgtgg gcgtgaccgc cacgagctcg cccgcacgcg cgctcacgtt
     9781 gccggcagtg cgctccacga gcccggcgtc ggccaggcgg cgcgccgccg ccgccaccgc
     9841 ggcgcgctca ctcgccagct ttctcgcccc gctcgcaccc atcgcagccg gcgatgttac
     9901 ggccgcgtgc atacttcggc gccatgacct acatcgcccg agaggaccgc tacgagcgga
     9961 tgctgtaccg gcgctgcggc cgcagcggga tcgatctgcc cgccgtgtcg ttcggactct
    10021 ggaacaactt cggcgacgac cgcccgatcg agacgcagcg cgcgatcctg cggcgcgcgt
    10081 tcgacctcgg cgtcacccac ttcgacctcg ccaacaacta tgggccgccg tacggctccg
    10141 cggaactgaa cttcggccga atcatgcgcg aggacctgcg gccctaccgg gacgagctga
    10201 tcatctccac caaggcgggc tacgacatgt ggcccgggcc ctatggcgag tggggctcgc
    10261 ggaagtacct gctagcgagc ctggaccaga gcctccagcg gatgggcctc gactacgtcg
    10321 acatcttcta ctcgcaccgc cacgatccga acacgccgct cgaggagacg atgagcgcgc
    10381 tcgagacagc ggtgaagcag ggcaaggcgc tgtacgcggg gatctcgtcg tactcggggg
    10441 cgcgcacgaa agacgcgttc gagatcttgc acaaccgcgg cattcggctg ctgatccacc
    10501 agccgtccta ctcgctcctc aaccgctgga tcgaggacga cctgctcgac gtgctcggcg
    10561 aggagggcat cggctgcatc gtcttctcgc cgctcgccca gggcatgctc acgagcaagt
    10621 acctcgacgg cgtgccggag ggatcgcgca tggccgaggg gcgcttcctg acgaccgaca
    10681 tgctcaccga ggaggctctc gggcgcatcc gcgcactcaa cgagatcgcg gcgcgccgcg
    10741 ggcagtcgct tgcccagatg gcgatcgcct ggacgctgcg cgacccgcgc gttacctcgg
    10801 cgctcatcgg ggcgagcagc gtcgagcagc tcgaggagaa catcgcggca ctcgacaacc
    10861 tcgacttcga cgacgacgag ctccacgaca tcgatcgcta cgcggtggac tcgggcatca
    10921 acatctgggc ggcatctagc gagcgctgac ggccgcgggc gcggtggcgc gccgaacctt
    10981 gagcgccatc agcggataga tcaggctcga gaggatcgcg gcgccaacca gtgctgctgc
    11041 ggtggtcgag cgcatgtggc ctgtttccgt agccagcgtg gtgaccgcca cgaccaacgg
    11101 cagctcggtg gcggtgaaca gggcgagtgc ggaccgctcg ggagcgtcga gatcgcggcg
    11161 atagatcaac agcgccggca gtcccctgac gacaaggaac agtgcgagga agaggggcag
    11221 cttcgcgagt gcggaggaac tgctcgtgag cccgtccaag tcgaacttca tgccactcgt
    11281 gacgaaaaag aacgggatca ggaacccgta gcccacggcc gtgagcttgg actcgaacag
    11341 atgtacctcc tgaccgcgaa gcgcctgtcg cgtgatgagt ccggccacga agccgccgag
    11401 cagcaggtcg agcccgagct ccgaagcaag cgcgacgaga gcgaaaacga gcaccaccgc
    11461 gagtcggacg gcgagctgac tgctcgactc gagcgtgcgc tcgagtacgc cccagccacg
    11521 ccctgccgag cggaccgcga acacgccggt gaggacggcg agtgcgacga acagcaccag
    11581 gatcacggct tcgtgcactg gatgtgaggt tgagaggacg agtgtgatga ggaggatcgg
    11641 gccaaattcg cccacggcgc cggcagagag caggtaggtg ccgaagcgcg tgtggagatc
    11701 gcccgagtcc cgcaggatcg ggatcagcgt gcccatcgcg gtcgtggcga gcgcggagcc
    11761 ggtatagagg aacgagatca caagtccggc cccctccagc gccgcaccga tgccgtacgc
    11821 gagcacgagc gagagcgccc agccgcgtgc gcccagctgc aggggtctgc cgcgaatgcg
    11881 ttcgaagtcg atctcgtagc cggcgaagaa gaagagcatt cccaggccga gattgccgaa
    11941 gaagtccgtg aattcgtcga cgtgcgcgat gtccgcgacc tgcggcccaa ccacgattcc
    12001 cagcacgagc tcgagcacca ccaccggcac cagcacacgg cttgcggcga acgtcgcgat
    12061 aacccccgcg agtgcggcca ccgcgacgat cacgaggaag gaagtggcgt ccacgccact
    12121 catatcgcga ctctagaccg ctggtactgg cgggacgagt cgggggccgc cgtgcgtctt
    12181 tctaggagga tccgccaaac aaacgcgcaa gggagcaggc gatgcggaag gctcgaattg
    12241 caggcacaac agttgcagtc gcggcggtgg ccatggcagt ggccggctac gcgacgagcg
    12301 tggccgacag cggcggcgcg ggcgccgcgg cggctcgtcc gaagccgacc gtggcgctgc
    12361 ggaccaccag cctcgggaag atcctggtcg acggcaagac cggccgcacg ctctacgagt
    12421 tcggcaagga ccgccgcaac aagagcaact gcagcggcag ctgcgcagcg atctggccac
    12481 cgctcaccgt atccggcaag ctgctcgccg ggaagggcgt ttccgcgtcg aagctgacga
    12541 cgatcaagcg ggccgacggc aagcgccagg ccgtctatgc gggtcacccg ctctatcgct
    12601 tcgcggcgga ttcgaaggcc ggccagacga acggcgaggg cgtgaacggc ttcggcggtc
    12661 tgtggtacgc gatgaatgcg aggggctcgc tggtgaaggg ccctcccccg tcgcaaccga
    12721 tgaacaaccc gtacggctac tgacggccgc cgtaggtgcg ccggcgcgcg tcgtcttcgc
    12781 cgacgacttg acggcgcgtc ggcggcccgg ggatgctggg cgcgtgtcat gagatgaggg
    12841 cggtcgtctg caggaacact gagctcgaag ttgtcgaccg gccagagccc gtgcccggac
    12901 gcgggcaggt gcgcatcgag gtgctgcgct gtgggatctg cgggtctgac ctgcatgcgc
    12961 gccacggctc ggacgactgg gccgacatgg cggcgcgcgt gggctacgac cggttcgctc
    13021 gctcgaacga acccgtcgtc ttcggtcacg agttctgtgg cgaggtcgtc gagcacggcc
    13081 cgcaatgccg gcgcggctca ccgactggca cgcacgtggt cgcgctgccg ctcctccgcg
    13141 ggacccacgg cgtagacacg attgggctgt cggtccacgc tccgggcggc tacgcggagc
    13201 agctgctggt ggaggagtcg ctcatgatgc cggtacccaa cggcctcacc cccgacgccg
    13261 cggcggtgac cgagccgatg gcggtggcct ggcacgccgt gcgccgtggc gaggtgggca
    13321 agcggacagt cgcgatcgtg atcggctgcg gtccgatcgg actggcggtg atccttgtcc
    13381 tgaaggccaa gggcgtccgg acggtcgtgg cgagcgacta ctcgcccggg cgtcgcgcac
    13441 tggccagcgc ttgcggtgcc gacatcgtcg tcgaccctgg ccatgagtcc ccgttcgcgg
    13501 ccgccgccgg ccgtcacctc aaggacctcc cctctgctct ggagctcgtg gtcggcacgc
    13561 gcgagaagct cgagcggctc cccgtcgggt ggtggcacgc gtggcggctc gctgaagcgc
    13621 ttggggccac gcccaagcac ccggtcatct tcgagtgcgt cggggtaccg ggcgtgatcg
    13681 agagcatcat cgacggcgca ccgctgctct cgcgcgtggt cgtggtcggt gtggtcgtcg
    13741 gcgtggacca catcacgccg gcgatggcca tcaacaagga gatcgacctg cggttcgcgt
    13801 tcgcctactc cccgctcgag ttccgcgatg cgttgcacat gctcgccgag ggagacatcg
    13861 accccgcgcc gctgatcacc ggcacggtcg gcctggaggg cgtcgacgcc gcgttcgccg
    13921 cgctcggcag ccccgagcgc cacgccaaga ttctcatcga cccgcgcagc tcggccgccg
    13981 agcccgttgc ttcagcgccc gttgcctaat tcgcgtggtg cgcagtcacc cgagtaccgg
    14041 catcagcgac gatccccttt gccgcgagcc gtccggtgaa cacgcagccg ccgaggaacg
    14101 tgccctcgag tgagcgcctg ccgtgcatcc caccgccgcc gaacccagcg gcctcgccga
    14161 ccgcgtagag accctcgacg ggctcgccat ccgcagtgag gacgcggcac tccaggtccg
    14221 tctggatgcc gccgaggctc ttgcgcgcca tcggctgcag gcggatcgcg atgagcggca
    14281 tcgctttctc gtcgacgatc cgggcgaagc gcgcggtgcg caggcggtca ccgcgccagt
    14341 tgcgaagttg agcgatgcgc cgaagctgat cgtcgttcca gaggctcttg ccgcgcccga
    14401 ggttgtcgtc gtagcgcgcg atctccaact cgagcaggtc agcgtcgagc gcgtcgtcgt
    14461 tcgtgacctc cgccatgcgc tgcgcgagct cgggcaccga cccggcggtc acgaaatcag
    14521 gacagccgtt caagaagtag cgcaccaggt tcggcttgcc gaaaagcacg ctgccgacga
    14581 agcgcccgag ccgcttctcg cgaaggtgcg ggttgtgctg tgagcccgaa acgtcgagct
    14641 cgcgcacggc gatcttccag ttgcacacga gccaggagta cttgcgctcg tcctcgcaca
    14701 tgcgctcgag cgcgtagtag gcatcgaacg tcggcatcac
//
LOCUS       DASVXS010000191         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_477203, whole genome shotgun sequence.
ACCESSION   DASVXS010000191 DASVXS010000000
VERSION     DASVXS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477203"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VF752_10550"
     CDS             complement(<1..1232)
                     /locus_tag="VF752_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856029.1"
                     /translation="MPPKRGTVLRVLAATAVVVALAIGGIFVALRVAGPSEYETAIGR
                     VSLKVQPRYHGRVDAFIPIADWGVRAHAFAAPVRLEIEPRTLDRQALLRAVGGDGTVL
                     ARAERDATRAAHRSLLRALLYAIGGGLAAGALLALILAAAGRRSRRALALGVALPTLA
                     AALLAGGALLRLQATFDSTAFEHPSFYARGAELNQLLNVAEKAQAGTKSYSSSVQRSI
                     AGYATLLSAGSRLTDVTAGPRALLASDFHNNVLVIKPLARLFSGGPAFVPGDFGQSGT
                     DAEARLLVPRVRALGNPVVAVSGNHDSRLIMRRLAAAGVVVLERDGRLLPNGQTDGQP
                     VQRIAGYTIAGFQDPLEWLGRDPNDPRRVFSFAQLPHGSQRYAKAQKDMNDWLDSLPE
                     RPQMVMIRENGLAQNLART"
     gene            complement(1311..2330)
                     /locus_tag="VF752_10555"
     CDS             complement(1311..2330)
                     /locus_tag="VF752_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8856030.1"
                     /translation="MSGARVGLIGYGLAGEVFHAPLIASEPGLDLALIVTGNPERRER
                     AERGHPDAAVLGTVDELWARAGELDLVVVAAPNRAHVPLAEAAIEARVPVVVDKPLAG
                     TAAEARRLVEAAERAGVMLTVFQNRRFDGDFLTARRLIEQGELGNVFRWESRFERWEP
                     QLEEGWRELAAAEEGGGVLLDLGSHLVDQALQLFGPARTVYGELDSRRAGAQVDDDAF
                     VAITHESGVRSHLWMSATAAQLGPRFRVLGDRAAFVKYGLDPQEPALAAGARPGDAGW
                     GLEPDELWGLLGTVDATQRVPTEAGAYERFYARVAAALRDDGPPPVDPRDSVAALEVL
                     DAARR"
     gene            complement(2327..2983)
                     /locus_tag="VF752_10560"
     CDS             complement(2327..2983)
                     /locus_tag="VF752_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8856031.1"
                     /translation="MPIDAVVFDMDGVLLHSEEVWNASKEAVIRENGGRWRDDAPRSM
                     MGMSSAEWSRYMHDELGVPLPPERIDELVVQRMRDTYEHALPLIPGADEAVRRIANRW
                     PLAVASSSNRPIIDLALELAGLDGCFQATVSSEEVGRGKPAPDVYLEAAARLGADPRR
                     CAAVEDSTNGILSAHAAGMKVIAIPNPTYPPEPHGLDAADVVLESIEQLTTEVVESLA
                     "
     gene            3065..3232
                     /locus_tag="VF752_10565"
     CDS             3065..3232
                     /locus_tag="VF752_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856032.1"
                     /translation="MIEYDRDPDEFDDLDEDLDEDEDVDGEDDDFAEELENDPAYNPP
                     DGEHLRDLKGG"
     gene            complement(3229..3417)
                     /locus_tag="VF752_10570"
     CDS             complement(3229..3417)
                     /locus_tag="VF752_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856033.1"
                     /translation="MSCPSCGLRMYSAGILLIRDECPRCGVELTRERAPKRAPVSLAD
                     LRRASAKGERPPEAPPPD"
     gene            3534..4310
                     /locus_tag="VF752_10575"
     CDS             3534..4310
                     /locus_tag="VF752_10575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX8856034.1"
                     /translation="MTRREIRSWLMDMDGVLVYEEQAIPGATAFLDHLRERELPFLVL
                     TNNSIYTRRDLSARLRASGLLVPEESIWTSALATARFLEDQRPGGSAFVIGESGLTTA
                     LHQAGYTLTDRDPDYVVLGETRTYSFERITQAIRHVVNGARFIATNPDPTGPSPYGPL
                     PATGSVAALISRAAGVDPYYVGKPNPLMMRSALNALDAHSETTAMVGDRMDTDVIAGL
                     EAGLETVLVLTGVTTRPEAERFPYRPSRIVDSVADLVEEI"
     gene            complement(4312..4749)
                     /locus_tag="VF752_10580"
     CDS             complement(4312..4749)
                     /locus_tag="VF752_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX8856035.1"
                     /translation="MAKVLYTADAHVTGGRAAGHGRTSDGNLEVDLRLPREMGGNGDG
                     TNPEELFAVGFAACFEGALQVVARRERVDVGDAAIDSHVHLLPTEERGFKLAVDMDVS
                     LPQVEDSDQAIRMVQAAHQVCPYSNATRGNIDVNFTVNGREMS"
     gene            4875..5834
                     /locus_tag="VF752_10585"
     CDS             4875..5834
                     /locus_tag="VF752_10585"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8856036.1"
                     /translation="MSTAALRRNQHAQPFRHEALFYAGEAGFLGVTLPFIRDGVARGE
                     PILVVVSSRKIELLRAALADDTDGVLFADMGEVGANPARIIPAWRDFVEEHAEPGRRL
                     RGIGEPVYAERSPAELIECQRHEELLNLAFQDADDFWLVCPYDTDSLGPDILRDATLS
                     HPFGGASGNGNENPEYRGLDAAVAPLADPLPEPGNEVHELAFTGEGLGVVRGFVAEHA
                     ASAGLGQTRAGELVMAVNEAATNSVRHGGGRGLLRVWREDSSIICEVRDAGSIDAPLA
                     GRVRPRGAQAGGHGLWLVNQLCDLAQIRAAEDGGVVRLYMHAR"
BASE COUNT          865 a         2126 c         2095 g          855 t
ORIGIN      
        1 gtgcgcgcaa ggttctgcgc gagtccgttc tcgcggatca tcaccatctg cgggcgctca
       61 gggagggagt ccaaccagtc gttcatgtcc ttctgcgcct ttgcgtatcg ctgcgatccg
      121 tggggcagct gcgcgaagga gaacacgcgt cgcgggtcgt tcgggtcccg ccccagccac
      181 tcgagcgggt cctggaagcc cgcgatcgtg tacccggcga tgcgctggac cggctggccg
      241 tcggtctgtc cgttcggcag cagccgcccg tcgcgctcga gcacgaccac acccgctgct
      301 gcgaggcggc gcatgatcag gcgcgaatcg tggttgccgg acaccgcgac gactgggttg
      361 ccgagggccc gcacgcgcgg gaccagcagc cgagcctcgg catccgtgcc ggactggccg
      421 aagtcgcccg gcacgaacgc ggggccgcct gagaagagcc gggcgagcgg cttgatcaca
      481 agcacgttgt tgtggaagtc cgacgcgagc agcgccctgg ggccggccgt gacatcggtg
      541 aggcggctcc cggcgctcag cagcgtcgcg tagccggcga tcgagcgctg taccgacgag
      601 ctgtacgact tcgtgccggc ctgggccttc tccgccacgt tgagcagctg gttgagctcg
      661 gcgccgcgcg cgtagaagct cgggtgctcg aaggccgtcg agtcgaacgt ggcctgaagg
      721 cgcaggagcg caccacctgc gagaagcgcg gccgccagcg tgggcagggc gactccgagc
      781 gcgagcgcgc ggcgcgagcg gcgacccgcc gcggcgagga tgagcgcaag cagcgcgcct
      841 gcggccagac cgccaccgat tgcgtacagg agcgcgcgca gcagcgagcg gtgggcggcg
      901 cgcgtggcgt cccgctcggc gcgcgccagc acggtgccat cgccgccgac cgcgcgcaga
      961 agcgcctgcc ggtcgagcgt gcgcggctcg atctcgagcc ggaccggggc cgcgaaggcg
     1021 tgggcacgga ctccccagtc cgcgatcggg atgaaggcgt cgacgcggcc gtggtaccgc
     1081 ggctgcacct tgagggacac ccggccgatc gccgtctcgt actcgctcgg acccgcgacc
     1141 cgcagcgcga cgaagatccc gccgatcgcg agggccacca ccacggccgt cgcggcgagc
     1201 acgcgcagca ccgttccgcg cttcggaggc acgaagcgct tcctcaagcg gctgcctgcc
     1261 cgcagcggcg ccctggggga ggccggggtt ggcactggcc ctcaagttac tcagcggcgc
     1321 gcggcgtcca gcacctcgag cgcggcgacg gagtcgcgcg gatcgaccgg tggtggcccg
     1381 tcatctctca gcgcggccgc cacgcgggcg tagaagcgct cgtaggctcc ggcctccgtc
     1441 ggcactcgct gcgtcgcgtc gacggtgccg agcagtcccc acagctcgtc gggctcgagc
     1501 ccccagcccg cgtcgcccgg gcgtgcgccg gcggcgagcg cgggctcctg cgggtcgagc
     1561 ccgtacttca cgaacgccgc cctgtcgccg agcacccgga agcgcggccc gagctgcgcg
     1621 gcggtcgcgc tcatccacag gtgcgagcgc acgccgctct cgtgggtgat cgcgacgaac
     1681 gcgtcgtcgt ccacttgggc gccggcgcgg cgcgagtcga gctcgccgta cacggtacgc
     1741 gcggggccga agagctgcag cgcctggtcc accagatggc tgccgagatc gagcagcact
     1801 ccaccgccct cctccgcggc tgcgagttcg cgccagccct cttcaagttg aggctcccag
     1861 cgctcgaagc gcgactccca gcggaacacg ttcccgagct cgccctgctc gatcaggcgc
     1921 cgggccgtga ggaagtcgcc gtcgaagcgc cggttctgga acaccgtgag catcacgccc
     1981 gcccgctcgg cggcctccac cagccgcctc gcctcggccg ccgtgccggc gagcggcttg
     2041 tcgacgacga ccgggacgcg cgcttcgatc gccgcttcgg cgagcggcac gtgggcccgg
     2101 ttgggcgccg cgaccacgac caagtcgagc tcgccggcac gcgcccagag ctcgtcgact
     2161 gttccgagta cggcagcgtc cggatggcct cgctccgcgc gctcgcggcg ctcggggttg
     2221 ccggtgacga tcaacgccag atcgaggccg ggttcggagg cgattagcgg cgcgtgaaac
     2281 acctcgcccg ccaggccgta gccgatcagc ccgacgcgcg ccccgctcac gcgagcgact
     2341 ccacgacctc ggtcgtcagc tgctcgatcg actcgagcac cacatcggcc gcatcgaggc
     2401 cgtgcggctc cgggggatag gtggggttag gtatcgcgat caccttcatg cctgcggcgt
     2461 gcgcgctgag tatgccgttc gtcgagtcct cgacggcggc gcaacgacgc gggtcggcgc
     2521 cgagcctggc tgcggcctcg aggtacacgt cgggcgcggg cttgccgcgg cccacctcct
     2581 cggacgagac cgtcgcctga aagcagccgt cgagtccggc cagctccagc gcgaggtcga
     2641 tgatcgggcg gttcgacgac gacgccaccg cgagcggcca ccgattcgcg atccggcgca
     2701 ccgcctcatc ggcgccggga atcagcggca gtgcgtgctc gtaggtgtcg cgcatccgct
     2761 gcaccacgag ctcgtcgatc cgctcgggcg gcagcgggac gcccagctcg tcgtgcatgt
     2821 agcgcgacca ctcagccgag ctcatgccca tcatcgagcg cggcgcgtca tccctccagc
     2881 gcccgccgtt ctcacggatc accgcctcct tcgatgcgtt ccagacctcc tcggaatgga
     2941 gcagcacgcc gtccatgtcg aataccaccg cgtcgatcgg caccggggca acttatcccg
     3001 ctggaggcgt cctgggatca tttgccgcgt agccgcggcc gccacccgac ccccaggagg
     3061 caccgtgatc gagtacgacc gagacccgga cgagttcgac gacctcgacg aggatctgga
     3121 cgaggacgag gacgttgacg gcgaggacga cgacttcgcc gaggagctcg agaacgaccc
     3181 cgcgtacaac ccgccggacg gcgagcacct gagggatctg aagggcggct agtccggcgg
     3241 tggcgcctcg ggcgggcgtt cgcccttcgc ggacgcccgc cgcagatctg cgagcgagac
     3301 cggcgctcgc ttgggcgccc gctcgcgcgt gagctcgaca ccgcagcggg ggcactcgtc
     3361 gcggatcagc aggatgcctg cgctgtacat gcgaaggccg cagctggggc aggacatgaa
     3421 cggcacctgg atggccagcg taaggtcggc cgtaggggcg gggcaagcga agtacttgtg
     3481 tgcgcgcgcc atccggtggg cgtccgctcc gacgcaatcc gtacgatgcg ccggtgactc
     3541 ggcgagagat ccgctcctgg ctcatggaca tggatggcgt tctcgtctac gaggaacagg
     3601 cgatcccggg tgcgaccgcg ttcctcgacc acctgcgcga gcgcgagctg ccgttcctgg
     3661 tcctcaccaa caactcgatc tacacccgcc gcgacctctc ggcgcggtta cgcgccagcg
     3721 ggctgttggt gcccgaggag tcgatctgga cctctgcgct cgcgaccgcc cgctttctcg
     3781 aggaccagcg gcccggcggt tcggcgttcg tgatcggcga gtctgggctc accacggccc
     3841 tccaccaggc gggctacacg ctcacggacc gcgaccccga ctacgtggtg ctcggcgaga
     3901 cgcgcaccta cagcttcgag cgcatcacgc aggcgattcg tcacgtggtg aacggcgcgc
     3961 gcttcatcgc cacgaatccc gacccgaccg gcccgtcgcc ctacggcccg ctgccggcga
     4021 ccgggtccgt ggccgcgctg atcagccgcg ccgcgggcgt cgatccttac tacgtcggca
     4081 agccgaatcc cctgatgatg cgctcggcac tgaacgcgct cgacgctcac tccgagacga
     4141 cggcgatggt cggcgaccgc atggacaccg acgtgatcgc cggactggag gctggcctcg
     4201 agaccgtgct ggtgctcacc ggggtgacca ctcgcccgga ggctgagcgc ttcccgtata
     4261 ggccgtcgcg gatcgtggac tcggtcgccg acctggtcga ggagatctga ctcagctcat
     4321 ctcacggccg ttgaccgtga agttaacgtc gatgttgccg cgcgtggcgt tcgagtacgg
     4381 gcagacctgg tgggcggcct gcaccatccg gatggcctga tcgctgtcct cgacctgggg
     4441 gagcgagacg tccatgtcca cggcgagctt gaagccgcgc tcttccgtcg gcagcaggtg
     4501 cacgtggctg tcgatcgccg cgtcgccgac gtccacgcgc tcgcgccgcg ccaccacctg
     4561 tagcgcgccc tcgaagcagg ccgcaaaacc gactgcaaac agctcttcgg ggttcgtgcc
     4621 gtcgccgttg ccgcccatct ccctggggag acggaggtcc acctccaggt tgccgtcgct
     4681 cgtgcgcccg tggccggcgg cacgcccgcc cgtgacgtgc gcgtcggcgg tgtagagaac
     4741 cttcgccatg gagttccttt cgtcgcctga cccgtggcca cggagaatat ggcgtcggca
     4801 cactttcccc cacgcgccac cctgcggcga gacacggtgt tctcgaccac ccgcctgggt
     4861 aagggtgaac cgggatgtct accgccgccc tcaggcgaaa tcagcacgct cagcccttcc
     4921 gccacgaggc cctgttctac gcgggcgagg ccggattcct aggcgtgacg ctgccgttca
     4981 tccgtgacgg agtggcacga ggcgagccga tcctcgtggt ggtgagctcg cgcaagatcg
     5041 agctgctgcg cgccgcgctc gccgacgaca ccgatggagt cctcttcgcc gacatgggcg
     5101 aggtgggagc gaacccggcg cggatcattc ccgcctggcg cgatttcgta gaagagcacg
     5161 cagaaccggg acggcggctg cgcgggatcg gcgagcccgt gtatgccgag cgcagcccgg
     5221 ccgagctcat cgagtgccag cgccacgagg agcttctgaa tctcgcgttc caggacgccg
     5281 acgacttctg gctcgtgtgc ccttacgaca cggattcact cggcccggac atccttcgag
     5341 acgccacgct cagccatccg ttcggcggcg cgtcaggaaa cgggaacgag aacccggagt
     5401 accgcgggct cgatgctgct gttgcgccgc tcgccgaccc ccttcccgag cccggcaacg
     5461 aggtgcacga gctcgcgttc acgggcgagg gcctgggcgt cgtgcgaggc ttcgtcgcgg
     5521 agcacgccgc gtcggcgggc ttagggcaaa cgcgcgcagg cgagctggtc atggcggtga
     5581 acgaggcggc cacgaacagc gtccggcacg ggggagggcg cgggctgctg cgcgtgtggc
     5641 gcgaggactc gtcgatcatc tgcgaggtcc gcgacgcggg gtcgatcgac gcgccgctgg
     5701 cgggccgggt gcgtccgcgg ggcgcgcagg ccggcgggca tggcctatgg ctggtgaacc
     5761 agctgtgcga cctcgcccag atccgcgcgg ctgaggacgg cggcgtggtg cggctgtaca
     5821 tgcacgcccg ttaggacgcg cgctctcttt ccgcctcctc ctcgcgcgcc gaagcggtcg
     5881 ccgcggcagg ccggcgagcc ccgaccggga ccggcgccgg tctcagccgc atcagcttcc
     5941 g
//
LOCUS       DASVXS010000192         7160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_477212, whole genome shotgun sequence.
ACCESSION   DASVXS010000192 DASVXS010000000
VERSION     DASVXS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477212"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2147)
                     /locus_tag="VF752_10590"
     CDS             complement(<1..2147)
                     /locus_tag="VF752_10590"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX8856037.1"
                     /translation="MRTPTEAHVELPISGMTCASCANRIERKLNKLDGVQASVNFATE
                     RATVEFDSARVEPSELVEAVEAAGYSATLPSAEAATPAEADDPTAPLRLRLIVSALLG
                     LPVLFVSTIPALQFDNWQWLALQLATPVVIWGAWPFHRAAWANLRHGTATMDTLVSLG
                     VLAAWLWSLYALFIGDAGMRGMKMAFDIVPSRRGGDELYLEVASVVTVFMLAGRYFEA
                     RAKRRAGAALEALLELGAKDVAVLDEDGSERRVPTDQLHVGDRFVVRPGEKVATDGVV
                     EEGQSAVDQSLLTGESVPVEKAPGDEVAGATVNAGGRLVVRAARVGADTALAQIARLV
                     TDAQSGKAPVQRLADRVAGVFVPIVIGLAVAAGGFWLGAGESAAFAFTAAVSVLIIAC
                     PCALGLATPTALLVGTGRGAQLGLLIKGPEVLESTRQVDTVVLDKTGTVTSGHMQLLD
                     VLTAPGTERVDALRIAGALEDASEHPIARAVADAARAGAGTLPAVEQFANREGLGVEG
                     VVDGHRVQVGRPALMEEWSLRLPPALAAARRAAEDAGRTAVVAAWDGEVRALLVVADT
                     VKATSAEAVASLKALGLRPILLTGDNEVTARAVARQVGIDEVIAEVLPSEKAAVVRRL
                     QGEGRAVAMVGDGVNDAPALAAADLGIAIGTGTDVAIEASDLTIVSGDLRGAADAIRL
                     SRSTLRTIKQNLCWAFGYNVAAIPLAAAGLLNPV"
     gene            complement(2147..3004)
                     /locus_tag="VF752_10595"
     CDS             complement(2147..3004)
                     /locus_tag="VF752_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856038.1"
                     /translation="MSTWARLAAFAAVLVVAFASAVGAGKAIDPDTGGREDSHAKEKQ
                     MAEMTEMKTGHGAHSAEAAGLAVAEKGLRLVAAETTLQAHRSTRYDFRIAREDGSTVR
                     DFDVEHSRRMHMIVVRRDLTGYEHVHPRQRADGSWTVNLRLNDPGVYRVYADFGTRGA
                     SHTLATDVFVPGRFSPEPLPAAAESATGDGYNVALERHGDELFFRVTRDGRPVDVQPY
                     LGADGHLVALRQGDLAFLHVHPSDDHATAKGAIAFHAEYPSKGSYRLFLQFKHRDSVH
                     TVAFTQEVR"
     gene            complement(3001..3207)
                     /locus_tag="VF752_10600"
     CDS             complement(3001..3207)
                     /locus_tag="VF752_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX8856039.1"
                     /translation="MSTTTTEYRVVGMTCGHCVASVTEEVSELPEVTAVEVELDSGRL
                     RVAGTASDEAVAAAVAEAGYEVTA"
     gene            3314..3613
                     /locus_tag="VF752_10605"
     CDS             3314..3613
                     /locus_tag="VF752_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX8856040.1"
                     /translation="MPAETTTPTRGYTATKDDLLKRLRRIEGQVRGVEGMVEDDRYCI
                     DVITQISAIQAALDKVALGLLDDHTQHCVMGAKGDDQREKTDELMAAVGRLMRRG"
     gene            complement(3705..4223)
                     /locus_tag="VF752_10610"
     CDS             complement(3705..4223)
                     /locus_tag="VF752_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8856041.1"
                     /translation="METSAQQQQQSDGEYVIYKGHPSWRGILSFYIKGTAVALVAGAL
                     AALVTRIANDHVQWGWVVLVFGVVLGLVVLAGLIKRIATTYTVTSRRLTIREGLLSKR
                     TKETRLERVQNVNTEQSPVDRLLRVGKVDFDTAGSDDYEFAFVGISDPEGIARKVDHA
                     AHEERATLRDGL"
     gene            4480..5136
                     /locus_tag="VF752_10615"
     CDS             4480..5136
                     /locus_tag="VF752_10615"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX8856042.1"
                     /translation="MLKLVIAVAAIGLPDSLNPSLIAGALYYAAGREPARHTAAFTAG
                     AFLTTLAGGAVIALGLGHLVLSLLPELSSDTKDSLLVAGGAVLIVGGTAIWVRRDALV
                     RRTPQPRARGRSAFLVGVGIAGVELFTAFPYFASIALIVGAGVSLPGKTIALVTFNVL
                     YVLPLIGITVVCAVMGSHAERVLAPFRRWALTRWPHVVAPAAVVLGGVVAGYGIARLA
                     "
     gene            complement(5112..5591)
                     /locus_tag="VF752_10620"
     CDS             complement(5112..5591)
                     /locus_tag="VF752_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8856043.1"
                     /translation="MSKLMSVNRPMKEPIGLRVTRTAKALSRAFDQELAAAGGTLPTW
                     LILLSVKSGRAATQRELAKAVGIQGATLTHHLTALESAGLISRTRDPSNRRVQRVELT
                     EAGDAAFLRLRQAAQRFDARLRQGLDEDEVDQLRELLGRLDANVGGAQPAQASRAMP"
     gene            5622..7088
                     /locus_tag="VF752_10625"
     CDS             5622..7088
                     /locus_tag="VF752_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX8856044.1"
                     /translation="MDTQHTSQGSLPDGADAAGGSVRLAFMALVLAMLPAVLDQTILA
                     TGLPTIARDLGTLSDVSWLVAAYVVAATAVTPVWGKLGDRLGHKPMLQLSLAGFVTAS
                     ALCGLAQDITQLVVTRAVQGVFAGGLMTLAMAAVGDLVSMRERGRYQGYIAATFAGAT
                     VLGPLVGGVLVEHASWRWVFYVNLPVGVAALAALTAKLPAAAAGGERKPLDVLGAALL
                     AGATSALLLMCVWGGDRYAWDSAQIVGLAASALVLAGLLVLRERRAVDPIVPFDVLAK
                     PAVAVASAGLFLATACIFAVTVFVPVFLQAARGIGATEAGLLLVPMMAGITLSTTVAG
                     RTIARTGRYKRFPVAGLGLMTAGLALLAVVTGGTSILATDAAIAVFGLGFGMVSQFLV
                     VAVQNAVDRTQLGIATAATGFFRALGGAIGAAALGAVFTARAAGGAGVADAVQTVFLV
                     AAPLAAIGLVIVLRLPEVPLQERRAPNGPPQARPAASS"
BASE COUNT          973 a         2570 c         2502 g         1115 t
ORIGIN      
        1 acggggttca gcaaccccgc cgcggcgagc gggatcgccg ccacgttgta gccgaacgcc
       61 cagcacaggt tctgcttgat cgtgcgcagc gtcgagcgcg agaggcggat cgcgtccgct
      121 gctccgcgca ggtcgcccga cacgatcgtg aggtccgagg cctcgatcgc gacgtcggtg
      181 ccggtgccga tcgcgatgcc gaggtcggcc gcagcgagcg cgggcgcgtc gttcacgccg
      241 tcacccacca tggccaccgc gcggccctcg ccctgcagcc gccgcaccac ggcggccttt
      301 tcggacggaa gcacctcggc gattacctcg tcgatcccga cctgccgagc gaccgcgcgc
      361 gctgtcacct cgttgtcgcc ggtcaacagg atcggtcgaa gcccgagcgc cttcagcgac
      421 gccacggcct ccgccgacgt cgccttgacg gtgtcggcga ccaccaacag agcgcgcact
      481 tcgccgtccc acgcggcgac gacagcggtg cgcccggcgt cctcggcggc acggcgtgcg
      541 gcagcgaggg cgggcggcag gcgcaggctc cattcctcca tcagcgccgg ccggcctacc
      601 tgcacgcggt gtccgtcgac cacgccctcc acgccgagcc cctcgcggtt cgcaaactgc
      661 tccaccgccg gaagcgtgcc agcccctgcg cgggcagcgt cggccacggc acgcgcgatc
      721 gggtgctcgg acgcgtcctc gagggcgcca gcgatgcgca gtgcatcgac gcgctcggtg
      781 cccggcgccg tcaacacgtc cagcagctgc atgtgcccgc tcgtgacggt gccggtcttg
      841 tcgagcacca cggtgtccac ctgacgggtc gactcgagca cctccggccc cttgatcagc
      901 aggccgagct gcgcgccacg gcccgtgccg acgagcagag ccgtcggggt ggcgagaccg
      961 agtgcgcacg ggcaggcgat gatcagaacg gagaccgccg cggtgaacgc gaacgcggcg
     1021 ctctcgcccg cgccgagcca gaagccgccc gcggcgacgg ccagcccgat cacgatcggc
     1081 acgaacacgc cggccacacg gtcggcgagg cgctgcaccg gggccttgcc cgactgcgcg
     1141 tccgttacca gccgtgcgat ctgggcaagc gcagtgtcgg cgccgaccct ggcggcgcga
     1201 accaccaagc ggccgccggc gttcaccgtc gcgcccgcga cttcgtcgcc tggtgccttc
     1261 tcgacaggga cggactcgcc ggtgagaagc gactggtcga cggccgactg gccctcctcg
     1321 accacaccgt cggtcgcgac cttctctccc ggccgcacca cgaagcggtc gccgacatgc
     1381 agctggtcag tcggaactcg ccgctcgctc ccgtcctcgt ccagtacggc gacgtccttc
     1441 gcgccgagct cgagcagcgc ctcgagcgcc gcgccggcgc gccgcttcgc gcgcgcctcg
     1501 aagtagcgcc cggcgagcat gaacacggtc acgacggacg cgacctccag gtagagctcg
     1561 tcgccgcccc tgcgcgaggg gacgatgtcg aaggccattt tcatcccgcg catgccggcg
     1621 tcgccgatga agagcgcgta cagcgaccaa agccacgcgg ccagcactcc cagcgacacg
     1681 agcgtgtcca tcgtggctgt cccgtgcctc aggttcgccc aagccgcacg gtggaacggc
     1741 cacgcgcccc agatcacgac gggcgtggcc agctgaagcg ccagccactg ccagttgtcg
     1801 aactgcagcg ccgggatcgt cgagacgaac agcacgggca ggcccagcag cgccgacacg
     1861 atcagccgca ggcgcagtgg ggccgtgggg tcgtccgcct cggccggcgt tgcggcttcg
     1921 gcgctcggca gcgtggcgct atagccggcc gcctcgaccg cctccacgag ctcgctcggc
     1981 tcgactcgcg cggagtcgaa ctccaccgtg gcgcgctcag tggcgaagtt cacgctcgcc
     2041 tgcacgccgt cgagcttgtt cagcttgcgc tcgatccggt tcgcgcacga ggcgcaggtc
     2101 atgccgctga tcggcagctc cacgtgcgct tcggtaggcg tgcgcatcag cgcacctcct
     2161 gcgtgaacgc gacggtgtgc acgctgtcgc ggtgcttgaa ctggaggaag agccggtagc
     2221 tcccctttga cgggtactcg gcgtggaagg cgatcgcccc cttcgccgtg gcgtggtcat
     2281 cgctcgggtg aacgtgcagg aaggcgaggt cgccttggcg cagcgccacg aggtggccgt
     2341 cggcgccgag gtacggctgc acgtcgaccg gtcgcccgtc gcgggtcacg cggaagaaga
     2401 gctcgtcgcc gtgcctttcc agcgcgacgt tgtagccgtc cccggttgcc gactccgcgg
     2461 ccgccggtag cggctcgggc gagaatctgc ccggcacgaa gacgtccgtg gccagtgtgt
     2521 gagacgcgcc gcgcgtgccg aagtccgcgt acacgcggta cacgcccggg tcgttcaaac
     2581 gcaggttcac ggtccagctc ccgtccgcgc gctgccgcgg atgcacgtgc tcgtacccgg
     2641 tcaggtcccg gcgcaccacg atcatgtgca tgcgccggga gtgctcgacg tcgaagtcgc
     2701 gcacggtaga gccgtcctca cgcgcgatcc ggaagtcgta tcgagtgctg cggtgcgcct
     2761 ggagagtggt ctcggccgcc accagcctca ggcctttctc ggccacggcg agtccggccg
     2821 cttcggcgga gtgggcgcca tggccggtct tcatttcggt catctcagcc atctgttttt
     2881 ccttcgcgtg cgagtcctcg cgaccgcccg tatccggatc gatcgcctta ccggcgccca
     2941 cggcggaggc aaaggcgacc acgagcaccg ctgcgaatgc ggcgagtcgg gcccaagtgc
     3001 tcacgcggtg acctcgtagc ccgcctcggc gacggcggct gcgacggcct cgtcgcttgc
     3061 ggtccccgcg acgcgcaggc ggccgctgtc gagctcgacc tccacggcgg tcacctcggg
     3121 cagctccgac acctcttcgg tgaccgacgc gacgcagtgg ccacaggtca ttccgacgac
     3181 gcggtactcg gtggtggtcg tgctcatctc gctgtgctcc tacatacagg ggaggggtat
     3241 gcgaggcaca acgtagcaca tacccccagg gggtagtagt atggctatcc acactttcga
     3301 tttgaggagc acgatgccgg ccgaaaccac cacccctacg cggggctaca cagcgacgaa
     3361 agatgacctg ctcaagcgcc tgcggcgcat cgaagggcag gttcgcggcg ttgagggcat
     3421 ggtcgaggac gaccgctatt gcatcgacgt gatcacgcag atctccgcga tccaggccgc
     3481 gctcgacaag gtcgcgctag ggctgctcga cgaccacacc cagcactgcg tgatgggtgc
     3541 caagggcgac gaccagcgcg agaagaccga cgagctgatg gcggccgtcg gccgcctcat
     3601 gcgccgggga tagagaaggc ccggactccg gtccagctac ggccggtttg aggcgctgcg
     3661 aacgccgcga agcgctggat cctcgccaag cgggcccggg caggtcagag accgtcgcgg
     3721 agcgtcgcgc gctcctcgtg cgcggcgtgg tcgaccttgc gggcgatgcc ctccgggtct
     3781 gagataccga cgaacgcgaa ctcgtagtcg tcggagccgg cggtgtcgaa atcgaccttg
     3841 ccgacccgca ggagccgatc gacgggactc tgctcggtgt tcacgttctg cacgcgctcc
     3901 agccgtgtct ccttcgtccg cttcgagagg aggccctcgc ggatggtcag gcgtcgcgag
     3961 gtgaccgtgt acgtggtcgc gatccgcttg atcagcccgg cgagcaccac gagcccgagc
     4021 accacgccga acaccagcac gacccagccc cattgcacgt ggtcgttcgc aatccgggtg
     4081 acgagcgccg caagcgcgcc ggccacgagc gcgacggcgg tgcccttgat gtagaagctg
     4141 aggatgcccc gccaggacgg gtggcccttg tagatcacgt actcgccgtc gctctgctgt
     4201 tgctgctgcg cgctcgtctc catgcggcga ccgtaccagc ggccgggcgg gggtcaagct
     4261 ctgcctgcaa aagccgatct tctaggtgga gatggtccac cggagcagca tgcggatcgc
     4321 ggtggtagcg gcagtcgcga cggcggccgc gatggtcgca acaggcacgg cgcgcgctga
     4381 gcacgtcaag gcgccgcccc cggcgccact tgcgaagcag cccgcgccgg cgcccttcac
     4441 ccgctagtca gggtcgcgcg gcatgatgcc gccgaaccca tgctcaagct ggtcatagcc
     4501 gtcgccgcga tcgggctgcc cgactcgctg aacccgtcgc tgatcgcggg cgcgctctat
     4561 tacgcggccg ggcgagagcc ggcgcggcac acggcggcct tcacggcggg cgccttcctc
     4621 acgaccctcg ccggcggagc ggtcatcgcg ctgggtctcg ggcacctggt cctgtcactc
     4681 ctacccgagc tgagctcgga cacgaaggac tcgctgcttg tcgcgggcgg cgccgtcctg
     4741 attgtcggag gcaccgcgat ctgggtgcgg cgcgatgcgc tcgtccggcg cacgccgcag
     4801 ccgcgagcgc ggggccgctc ggcgttcctc gtcggcgtcg ggatcgcggg cgtggagctg
     4861 ttcacggcgt tcccgtactt cgcgtcgatc gcgctcatcg tgggcgccgg cgtgtcgctc
     4921 ccgggcaaga cgatcgcgct cgtgaccttc aacgtgctct acgtgctgcc gctgatcggc
     4981 atcaccgtgg tgtgcgccgt gatgggcagc cacgccgagc gggtgctcgc accgttccgc
     5041 cgctgggcgc tcacgcgctg gcctcacgtg gtcgcgccgg cggccgtggt gcttggcggg
     5101 gtggtcgccg gctacggcat cgcgcggctc gcctgagccg gctgggcgcc gcccacgttg
     5161 gcgtcgaggc ggccgagcag ctcgcgcagc tggtcgacct cgtcctcgtc caggccctgg
     5221 cgcaggcggg cgtcgaagcg ctgtgccgcc tggcggagcc ggagaaacgc agcgtcgcct
     5281 gcctccgtca gctccacgcg ctggacgcgc cggttgctcg ggtcgcgcgt gcgcgagatc
     5341 aggccggcgc tctcgagcgc ggtgaggtgg tgcgtgagag tggcgccctg gatgcccacc
     5401 gccttcgcca gctcgcgctg ggtcgcggcc ctgccgctct tcaccgagag caggatcagc
     5461 caggtgggca gcgtgccgcc cgcggctgcc agctcctggt cgaaggcgcg gctcagcgcc
     5521 ttggcggtcc gcgtcacgcg gaggccgatc ggctctttca tcggtcggtt caccgacata
     5581 agcttagaca tctaacgatt agatgtctaa cattcctcca catggacaca caacacacat
     5641 ctcagggcag tctccccgac ggcgccgacg ccgcgggagg cagcgtgcga ctcgcgttca
     5701 tggccctcgt tctggcgatg ctccctgcgg tgctcgatca gacgatcctt gccaccggcc
     5761 tgcccacgat cgcccgcgac ctcggcactc tgagcgacgt gtcgtggctc gttgccgcgt
     5821 atgtggtggc ggccaccgct gtcacgccgg tgtggggcaa gctcggcgac aggctcgggc
     5881 acaagccgat gttgcagctc tcgctcgccg gcttcgtcac ggcctccgcg ctctgcgggc
     5941 tcgcgcagga catcacccag ctcgtcgtga cccgagcggt gcagggcgtt ttcgccggtg
     6001 gcctcatgac cctcgccatg gcggccgtcg gcgacctcgt ctcgatgcgt gagcgcggcc
     6061 ggtatcaggg ctacatcgcg gcgacgttcg cgggcgccac ggtgctcggc ccgctcgtcg
     6121 gcggcgtgct ggtcgagcac gccagctggc gctgggtctt ctacgtgaac ctgcccgtgg
     6181 gcgtggcggc tctcgccgcg ctgacggcga agctgcccgc ggccgctgcc ggcggagagc
     6241 ggaagccgct cgacgtgctc ggcgccgcgt tgctcgcagg tgccacgagt gcgctgctgc
     6301 tcatgtgcgt gtggggtggc gaccgctacg cgtgggactc ggcgcagatc gtcgggcttg
     6361 cggcctcggc gctcgttctg gccgggctgc tcgtgctgcg cgaacggagg gcggtcgacc
     6421 cgatcgtgcc gttcgacgtt ctcgccaagc cggctgtggc tgtggcgagc gccgggctct
     6481 tcctcgccac cgcctgcatc ttcgcggtga cggtgttcgt gcccgtgttc ctgcaagcgg
     6541 cccgcggcat cggggccacc gaggctgggc tcctgctcgt gccgatgatg gccggcatca
     6601 cgctgtccac cacggtcgcc gggcggacca tcgcccgcac cgggcgttac aagcgcttcc
     6661 cggtcgctgg gcttgggctg atgaccgccg gcctcgcgct gctcgccgtt gtgaccggcg
     6721 gcacgtcgat ccttgcgact gatgccgcga tcgcggtgtt cggcctcggc ttcgggatgg
     6781 tgtcgcagtt cctcgtggtg gccgtccaga acgcggtcga ccggactcag ctcgggatcg
     6841 ccaccgccgc aaccggcttc ttccgcgcac tcggcggcgc gatcggagcc gcggcgctcg
     6901 gcgccgtgtt cacggctcgg gccgccggcg gcgcgggtgt ggcggacgcg gtgcagacgg
     6961 tgttcctcgt ggcggcgcca cttgcggcga tcgggcttgt catcgtcttg cggctccccg
     7021 aagtaccgct ccaggagaga cgggcgccga acgggccgcc acaagcgcga ccggcggctt
     7081 cgtcctgagg agccgtaggc tctgggccgt gccaacacga ccgcttgccg gcgtccgcgt
     7141 gctcgacctg tcgcgcgtgc
//
LOCUS       DASVXS010000193         6469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_194493, whole genome shotgun sequence.
ACCESSION   DASVXS010000193 DASVXS010000000
VERSION     DASVXS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194493"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VF752_10630"
     CDS             complement(<1..836)
                     /locus_tag="VF752_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8856045.1"
                     /translation="MLGPGVAAARATQALSRVARRGGTTLPGRVLLRMEPKAIDRLGE
                     RLAGGSVLVSATNGKTTTASMLSSALERAGRDLVHNRAGSNMNWGIATALLEADAGRD
                     QLGLFEVDEFWLPEMARALDPQLFLLSNLFRDQLDRYGELDLIADRWAAVVAERAGRS
                     RFVLNADDPLVADLGREREGVVYFGVEDDSHSLPELEHASDSKRCRNCGHEYVYDAVY
                     LAHMGRYHCPHCGRRRPDPSIAATRIELDGMRGSRVHVRTPQAAIELDLPLPGLYNVY
                     NAL"
     gene            complement(836..1717)
                     /locus_tag="VF752_10635"
     CDS             complement(836..1717)
                     /locus_tag="VF752_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8856046.1"
                     /translation="MTPRLALFVNPAAAGGRAVRALTPAIRELDARGAEYRVLQTEGL
                     GHARDQALEAAGSGETVVAIGGDGLVGCLAGALRHTDGRLAIVPGGRGNDLARVLGIP
                     TDPREAARVALDGEERLIDVAEANGAPFVGIASLGFDSDANRIANEAKLVKGNLVYLY
                     AALKALVQWRDASFTVTVNGGEPERVTGYSVAVANSKAYGGGMYLVPHAELDDGQLDV
                     LAVAGHSKLKVLWDLPKVFKGKHADSPFAHWSKGTAVRVNADRPFTVYADGDPIAELP
                     LTVTIDRRSLRVLVPRS"
     gene            complement(1714..2526)
                     /locus_tag="VF752_10640"
     CDS             complement(1714..2526)
                     /locus_tag="VF752_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX8856047.1"
                     /translation="MCRVLGCVARTPVSLRHELVEAPNPMIRQSEHHDSGWGMAVYPH
                     GDGEDPAVVRFPNAAHADGSFLDATDKHGRIFNVHVRRATMGGLTPENTHPFTLGSYS
                     FCHNGTILHFSRLLEPGVMEPAGETDSEHFFNLLMRDFDDGNVVGSLRRAVTFAIGSS
                     PFSGLNFLFSDGDRLYAYKLGGFDLHWLIRRDQTLVASERVTDESWHSVQDDVLITLD
                     PESPEDIHCERLVGDGVLASATIEKYKDGSELRGRERGDFAADRAARLAAGE"
     gene            2584..3117
                     /locus_tag="VF752_10645"
     CDS             2584..3117
                     /locus_tag="VF752_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856048.1"
                     /translation="MDTPTPKRIWWARPRKLCALERPGGGGRSHRPERRAAEIEYLRA
                     SGVRLVISTMATRHNLADYDAEGLDWHHVPVPGWAEAERALDELLPLLRRELKKRGAV
                     AIHGNRRTDFVAAVCAAHLHDARGLDPADGLAAAAEAGLTVSEEAAVLLGVPYGDLVQ
                     PRSAMAASTSSGLSVTT"
     gene            complement(3045..3332)
                     /locus_tag="VF752_10650"
     CDS             complement(3045..3332)
                     /locus_tag="VF752_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HEX8856049.1"
                     /translation="MPRKTHHKHSSKKGYRRLLDSLAALDGVHRVATGRITPRMGSGR
                     PVAPISKVRRTDSGLSITINADGAMVDAYVVTDKPDEVEAAMAERGWTKSP"
     gene            complement(3572..4387)
                     /locus_tag="VF752_10655"
     CDS             complement(3572..4387)
                     /locus_tag="VF752_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX8856050.1"
                     /translation="MALSPLAQRYDQFILDLDGCVWIGPNPIPGSIEAIAALREAGKR
                     VAFVTNNSRDSGETYVRKLWGMGVQASLMDVVTVGGAMQHLLAETRRNRTAFVLGTDA
                     MIEHVRSAGLKVLNGTDLASRADVVIVSGTDHMDYDDLKNASLAVSRGADFLASGRDS
                     TYPMPDGPWPGSGALLAAVEYTTGRTAAVVGKPQPQLMITALDRLGEGRTLVVGDRLD
                     TDIAAAAAAHLDSALVLTGHTTAEEAEEAKRRPKDEPRPIAVAPSLFELVVAG"
     gene            complement(4420..5157)
                     /locus_tag="VF752_10660"
     CDS             complement(4420..5157)
                     /locus_tag="VF752_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HEX8856051.1"
                     /translation="MPLVQDIHSDEVTAERGALVMYFVVRKDLPLGLGQAMAAAGAAA
                     VRCSRRFEEDERFAPVFAAWRESSFRKVALRAGADEFERVRAEHPSVDVGEWLLCLPP
                     LHRDDRSDLLASLRPFTDAPRPSESSPALPGDVPAMLYVIRPGVMKTAGKAMAQAGHA
                     SLMCESRWAQLASEEFAAWHDAGEPGEVVQADDAQWELLRDRCDGVVVRDGGLTQVEP
                     GTETVVALVPQPLDERPDVVRSLDRVP"
     gene            5184..5879
                     /locus_tag="VF752_10665"
     CDS             5184..5879
                     /locus_tag="VF752_10665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8856052.1"
                     /translation="MQTKTVVSLTLVALAVAGCGGSKNNKGSTSASADSGAPAQTTAQ
                     SSGAACQKVSAPSTGERKARQPRQKRDPGKTYTVTMQTNCGSFSFKLAVKTSPKASDS
                     IDSLVKKGFFDKTIFHRIVPDFVIQGGDPTASGTGGPGYKTVDSPSPSQDYPKGTVAM
                     AKAGDEAPGTAGSQFFVVTSDQGGAQLTPDYAVVGHVTKGMDVIGKIAALGSPTEQPT
                     QVVEIEKATVSEK"
     gene            5876..>6469
                     /locus_tag="VF752_10670"
     CDS             5876..>6469
                     /locus_tag="VF752_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8856053.1"
                     /translation="MSVYDVTEQDFMERVVERSHEVPVVVDFWADWCAPCRQLTPALE
                     NAARAREGQVDLAKLDVDANQMLAQSFRVQGIPAVKAFKDGRVASEFTGAIPPPQVEK
                     FFDALVPTEAEQIAHDEQAGEDELRRALALDPTQAPAAVRLARMLVERGADEEARTLL
                     EPLGNDFGAAGLLARIELDEAADPQLNEAFRAWDEGEH"
BASE COUNT          999 a         2243 c         2305 g          922 t
ORIGIN      
        1 agcgcgttgt agacgttgta gagcccgggc agcggcaaat cgagctcgat cgcggcctgt
       61 ggcgtgcgca cgtgcacgcg cgagccgcgc atgccatcga gctcgatgcg cgtcgcggcg
      121 atcgagggat ccggccgccg ccggccgcag tgcgggcagt ggtaacggcc catgtgcgcg
      181 aggtagaccg cgtcgtagac gtactcgtgg ccgcagttgc ggcagcgctt cgagtcggag
      241 gcgtgctcga gctccgggag tgagtgcgag tcatcttcga ctccgaagta gacgactccc
      301 tcgcgctcgc gcccaagatc ggccacgagc gggtcgtcgg cgttcaggac gaaccgcgaa
      361 cggcccgcgc gttcggccac caccgctgcc cagcggtccg cgatcaagtc gagctcgccg
      421 tagcggtcga gctggtcgcg gaagaggttg gagagcagga agagctgcgg gtcgagcgcg
      481 cgcgccatct cggggagcca gaactcgtcg acctcgaaca gcccgagctg atctcggccc
      541 gcgtccgcct cgagcagcgc cgtcgcgata ccccaattca tgttcgagcc ggcccggttg
      601 tggacgaggt cgcggccggc gcgctcgagc gccgacgaga gcatcgacgc ggtggtcgtc
      661 ttgccgttgg tcgcggacac gagcaccgat ccacccgcga gccgttcgcc caagcggtcg
      721 atcgccttcg gctccattcg cagcagcacg cggcccggca gcgtggtgcc gcctcgccgc
      781 gcgacgcggc tgagcgcctg ggtggcgcgg gctgctgcaa cccctgggcc cagcatcaag
      841 atctagggac tagcacgcgc aggctgcggc ggtcgatcgt gacggtcagg ggcagctcgg
      901 cgatcgggtc gccgtcggcg tagaccgtga acggccgatc ggcattcacc cgcacggcgg
      961 ttcccttcga ccagtgcgcg aacggagagt cggcatgctt gcccttgaag accttgggca
     1021 ggtcccacag caccttcagc ttcgaatgtc ccgcgactgc gagcacgtcg agctggccgt
     1081 cgtcgagctc ggcgtgcggc acgagataca tgccgccgcc gtacgccttt gagttcgcaa
     1141 ccgccaccga gtacccggtg acccgctcgg gctcgccgcc gttgaccgtg acggtgaagg
     1201 aggcgtcgcg ccactgcacg agcgccttca gggcggcgta gaggtagacg aggttgccct
     1261 tcacgagctt cgcctcattc gcgatccggt tcgcgtcgga atcgaagccc aggctcgcga
     1321 tgcccacgaa cggcgcgccg ttggcctccg cgacgtcgat cagccgctcc tcgccgtcga
     1381 gcgccacgcg cgcggcctcc cgcggatcgg tgggaatgcc gagcacgcgc gcgaggtcgt
     1441 tgccgcggcc gcccggcacg attgcgaggc ggccatcggt gtggcgtagc gctccggcga
     1501 ggcagccgac gaggccatca ccgccgatcg cgaccaccgt ctcgccgctg ccggcggcct
     1561 cgagcgcctg gtcgcgggcg tggccgaggc cctcggtctg cagcacccgg tattcagcgc
     1621 cgcgcgcgtc gagctcgcgg atcgcaggcg tgagcgcccg cacggcgcga ccgcccgcgg
     1681 ccgccgggtt cacgaacagc gcgagtcggg gcgtcactcg ccggccgcca gccgtgccgc
     1741 gcggtcggcg gcgaagtcgc cgcgctcccg gccgcgcagc tcgcttccgt ccttgtactt
     1801 ctcgatcgtg gcgcttgcca gcacgccgtc gccgacgagc cgctcgcagt ggatgtcctc
     1861 gggggactcc ggatcgagcg tgatcagcac gtcgtcctgg acgctgtgcc acgactcatc
     1921 tgtgacccgc tccgaggcca ccagtgtctg gtctcgccgt atgagccagt gcagatcaaa
     1981 tccgccgagc ttgtaggcgt acagccgatc cccgtccgaa aagaggaagt tcagcccgct
     2041 gaacggggag ctgccgatcg cgaacgtgac cgcgcgccgc agcgagccga ccacgttgcc
     2101 gtcgtcgaag tcgcgcatca gcaggttgaa gaagtgctcg gaatcggtct caccggcggg
     2161 ctccatcacg cccggctcga gcaggcgcga gaagtggagg atcgtgccgt tgtggcagaa
     2221 ggagtagctg ccgagcgtga atggatgggt gttttcgggc gtgaggccgc ccatcgtcgc
     2281 ccggcgcacg tgcacattga agatgcggcc gtgcttgtcg gtcgcgtcga ggaacgagcc
     2341 gtcggcgtgg gctgcgttcg ggaagcggac gacagcgggg tcctcaccgt cgccgtgcgg
     2401 gtagacggcc atgccccacc cggagtcgtg gtgctccgac tgccggatca tcggattcgg
     2461 cgcctccacc agctcgtgcc ggagcgagac cggcgtgcgc gctacgcagc cgagtacgcg
     2521 gcacatgggg gtgagttcca gtcgcccacg ccttgaagtc taaaagcgac gggaaggcgg
     2581 aggatggaca cacctacacc caagaggatc tggtgggcgc ggccgcgcaa actgtgtgcg
     2641 ctcgagcgac cgggcggggg cggacgcagc cacaggcccg agcgaagagc cgccgagatc
     2701 gagtatctgc gcgcgagcgg cgtccggctg gtgatctcga cgatggccac gcgtcacaac
     2761 ctcgcggact acgacgccga gggcctcgac tggcaccacg tgccggttcc cggctgggcc
     2821 gaggccgagc gtgcgctcga cgagctgctg ccgctgctgc ggcgcgagct gaagaagagg
     2881 ggcgcggtgg ccatccacgg caaccggcgg accgacttcg tggcggcggt ctgcgccgcc
     2941 cacctgcacg acgcgcgcgg cctcgatccc gccgacggtc ttgccgccgc cgcggaggcc
     3001 ggcctcaccg tgagcgagga ggcggcggtg ctgctcgggg tgccctacgg cgacttggtc
     3061 cagccgcgct cggccatggc ggcctccacc tcgtccggct tgtccgtgac cacataggcg
     3121 tcgaccatcg cgccgtcggc gttgatcgtg atcgagagac ccgagtcggt gcggcgcacc
     3181 ttcgagatcg gcgccaccgg ccggccggat cccatgcggg gcgtgatccg gccggtcgcc
     3241 acacgatgca caccgtcgag cgcggccagg ctgtcgagca ggcggcggta gcccttcttg
     3301 gacgagtgct tgtggtgcgt cttgcgtggc atggtggcgg ctcggcggtt cgaactgcgt
     3361 gcacttaagg atggcggacc ggggagtgtc ggccggtctt cctaacggga cctgggctgt
     3421 tttgacattg atagtcatgg tcaaaacggc ccagccggga cgtccgcgcc gcggagcccg
     3481 cggtgtcgtc acttaggggc cactacgggc ccaaaacgac gacgcggcgc gcaggtgagg
     3541 cgtcgactca ccgtccatta ctgctaaccc gtcaaccggc cacgacgagc tcgaacaggc
     3601 tgggggccac cgctatcggc cgcggctcgt ccttcggccg tcgcttcgcc tcctcggcct
     3661 cctccgccgt cgtgtggccg gtgagcacca gagccgagtc gagatgcgcg gccgcggcag
     3721 ccgcgatgtc ggtgtcgagc cggtcgccca cgacaagcgt gcgcccttcg ccgaggcggt
     3781 cgagcgccgt gatcatcagc tgcggctgcg gcttgcccac cacggcagcg gtcctgcccg
     3841 tcgtgtactc caccgcggcg aggagcgcgc cgctgccggg ccaagggcca tccggcatcg
     3901 ggtaggtgct gtctcgcccg gaggcgagga agtcggcgcc acggctcacc gcgagcgagg
     3961 cgttcttcaa gtcgtcgtag tccatgtggt ccgtgcccga gacgatgacc acgtcggcgc
     4021 gcgaggcgag gtccgtaccg ttcagcacct tcagccccgc cgagcgcacg tgctcgatca
     4081 tcgcgtccgt gccgagcacg aaggcggtgc ggttccggcg cgtctccgcc aacaggtgct
     4141 gcatcgcgcc gcccacggtc accacgtcca tcagcgacgc ctgcacgccc atgccccaga
     4201 gcttgcgcac gtaggtctcg ccgctgtcgc gcgaattgtt cgtcacgaac gcgacgcgct
     4261 tgcccgcctc gcgcagcgcg gctatcgcct cgatcgagcc cgggatcgga ttggggccga
     4321 tccagacgca gccgtcgagg tcgaggatga actggtcgta gcgctgggcg aggggggaga
     4381 gggccattgg cgcccgcatc gtatggagcg caccgggcgc tacggcacgc gatcgagcga
     4441 gcgcaccacg tcggggcgct cgtcgagggg ctgcggcacg agcgccacga ccgtctccgt
     4501 gccgggctcg acctgcgtga gcccgccgtc gcgcaccacc acgccgtcgc agcggtcgcg
     4561 aagcaactcc cactgcgcgt cgtccgcctg caccacctcg ccgggctcgc ccgcatcgtg
     4621 ccaggcggcg aactcctccg aagcgagctg cgcccagcgc gactcgcaca tcagcgacgc
     4681 gtggcccgcc tgcgccatcg ccttgccagc ggtcttcatc acgcctggcc ggatcacgta
     4741 gagcatcgcg ggcacgtcgc cgggcagcgc gggggaggac tccgagggtc gcggcgcgtc
     4801 ggtgaacggg cgaagcgacg cgagcagatc cgagcggtcg tcgcggtgga gcggcggcag
     4861 gcagagcagc cactcgccca cgtccacgga cgggtgctcc gcgcgtaccc gctcgaactc
     4921 gtccgcgcct gcgcgcagcg ccaccttgcg gaacgagctc tcgcgccacg cggcgaacac
     4981 gggcgcgaag cgctcgtcct cctcgaagcg gcggctgcag cgaaccgccg ccgcgcccgc
     5041 ggcggccatc gcctggccca gtccgagcgg caggtccttc cgtaccacga aatacatgac
     5101 gagcgcgccg cgctcggcgg tcacctcgtc ggagtggatg tcctgaacga gcggcatacg
     5161 gacgcttact actcttctcc gccgtgcaga cgaagacagt cgtttccctc accctcgtcg
     5221 cgctcgcggt cgcgggctgc ggcggcagca agaacaacaa gggcagcacc tcggcctccg
     5281 ctgacagcgg cgcgcccgcc cagaccacgg cccagtccag cggagccgcc tgccagaagg
     5341 tgtcggcgcc gagcaccggc gagcgaaagg cgaggcagcc gcgtcagaag cgcgatcccg
     5401 ggaagacgta tacggtcacg atgcagacca actgcggcag cttctccttc aagctcgccg
     5461 tgaagacatc gccgaaggcg agcgactcca tcgactcgct ggtgaaaaag ggcttcttcg
     5521 acaagaccat cttccacagg atcgtgccgg acttcgtgat ccagggcggc gatccgacag
     5581 ctagcggcac gggcgggccg ggctacaaga ccgtcgactc gccgtcgccc agccaggact
     5641 acccgaaggg cacggtcgcg atggcgaagg ccggcgatga ggcgcccggc acggcgggca
     5701 gccagttctt cgtcgtcacc tccgaccagg gcggcgcaca gctcacgccc gactacgctg
     5761 tcgtagggca cgtgaccaag ggcatggacg tgatcggcaa gatcgcggcc ctgggctcgc
     5821 ccaccgagca gcccacgcag gttgtggaga tcgagaaggc gacggtgtcg gagaagtgag
     5881 cgtctacgac gtcacagagc aggacttcat ggagcgcgtt gtggagcgct cgcacgaggt
     5941 gcccgtggtc gtcgacttct gggccgactg gtgcgcgccc tgccggcagc tcaccccggc
     6001 gctcgagaac gccgcacgtg cccgtgaggg gcaggtcgat ctcgcgaagc tcgacgtgga
     6061 cgcgaaccag atgctcgccc agagcttccg ggtgcagggc atcccggcgg tgaaggcgtt
     6121 caaggacggg cgcgtggcgt cggagttcac gggcgcgatc ccgccgccgc aggtggagaa
     6181 gttcttcgat gcgctcgtgc ccacggaggc ggagcagatc gcgcacgacg agcaggcggg
     6241 ggaggacgag ctgcgccggg cgctcgcgct cgacccaacg caggctccgg ccgcggtccg
     6301 cctggcgcgc atgctggtgg aacgcggggc cgacgaagaa gcgcgcacgc tgctcgagcc
     6361 gctcgggaac gacttcggcg ccgcgggact gctcgcgcgg atcgagcttg acgaggccgc
     6421 cgatccgcag ctgaacgagg ccttccgcgc gtgggatgaa ggcgagcac
//
LOCUS       DASVXS010000194         8596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_51562, whole genome shotgun sequence.
ACCESSION   DASVXS010000194 DASVXS010000000
VERSION     DASVXS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51562"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF752_10675"
     CDS             <1..268
                     /locus_tag="VF752_10675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8856054.1"
                     /translation="LEAMAAGVPVVATAVGGIPEIAADGTAELVAPGDDAALAAAIER
                     LLDDRDLAATQARAARERVLGRYTAAANARATLDLYERLLESRR"
     gene            265..1359
                     /locus_tag="VF752_10680"
     CDS             265..1359
                     /locus_tag="VF752_10680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856055.1"
                     /translation="MSVLMTVSDMSVGGAERMVAQLTVALHERGRETVVAAAPGPLDA
                     DLEAAGIERELLPRGSRSPLRAAAVVGALARVTARVSPELVHAHNPRIAASSAIAARV
                     GRPLARPPVLATFHGVLPSEYGVAARLLRLADHVVCVSAEIAHTLERSGVRGERLSVI
                     PNAVEAVEPLNPERRAALDSELGLGEAPVFSIVARLVPQKAHERFLEAARIVAESHPE
                     TRFLIVGDGPLRAQMEGLAGELNLGDQVLFTGVRADARDIIARSRALVFSSNWEGLSV
                     AALEALSAGVPVVSTDVEGMREMLGSGAGVMVGRDPAELAAAITRLIERPDDAEQMGT
                     IGRELARTVYSPPAMAAAYDNLYDGLIAAR"
     gene            complement(1356..3110)
                     /locus_tag="VF752_10685"
     CDS             complement(1356..3110)
                     /locus_tag="VF752_10685"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX8856056.1"
                     /translation="MRIRALPTVIATALALALPAGAQAAVAGHVRVAIDSNPSIAASS
                     QNTARSAVVILQPWQTGLLGQLKASDPGIKVLAYKNLGFISETASYSGISASGVTWPQ
                     AQAHEGWFLHDKAGKRFASWTWKWLWAADIGDPTYQQAFADNVIAELKETGFDGVMLD
                     DVSPTIKFQHDPGDVQEYSGDASYGRAMSDAIGYVGPRIQAAGKLVVANVASWTEYSS
                     VTTPWLDDLSGMLDEMFTKWGNRAGEGYSDQWQWERELAQVKDAEAKNKLFLGVAHGP
                     ASDRAAARFGWATTLLAGAGHSYFQVASDYTNEYWFPEFDYAIGEPTGAESRIADGVH
                     RRTFTNGLVVVNPTASDKSVSFGGSYSGSGLSGATGATLGAHCALILTRDGGNAGSPS
                     PTKGRSPRSSPSARRASSRLSVLATSASPGSVSVTWTRSKSGRRIGRYRVFRDGRLVG
                     TTHGRRWIDRAPVPGATHRYSVAAVTRSNRMLRASRAVAVHIASAAAAQASAGGAVSR
                     GVQTRLAAYAPRLFRAQYAERLVTAHGRAHWRRITRVKRPRSAATVRVTAPAGALVRI
                     VLVPRASGPALRSAALAY"
     regulatory      complement(3123..3197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3298..4119)
                     /locus_tag="VF752_10690"
     CDS             complement(3298..4119)
                     /locus_tag="VF752_10690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8856057.1"
                     /translation="MRRQPEGIRRHYEIERELSDRLRNSDWQERARLYSEVYDELFRR
                     VPDHPQNERKASAAAQAERMREELALLKPFLSRENTYLEIGAGDCALAFEVARRVARV
                     YAIDVSAEIAPREGNPANFELIISDGRSVPVAPGSVDVVFSDQLMEHLHPDDAREQLG
                     QIHGALRAGGRYVFITPNRLSGPHDISKHFDDEPTGFHLREYTNRELVALLRETGFER
                     VQAFVQVGPLHLRFPVAFVTAFETLLERLGGPGRRIARLPLARKVLGCRLAAVRA"
     gene            complement(4130..5311)
                     /locus_tag="VF752_10695"
     CDS             complement(4130..5311)
                     /locus_tag="VF752_10695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8856058.1"
                     /translation="MGEIAKPRALVVCPVVPYPPVGGGYKRTLRLLESMQRAGVQPHV
                     LTTDPGQPGAIDALRDLGFDVELLPGESGTIVSRLSQHAHRRPSPFLDSVDHRLRELS
                     TEGAAFVQLEHTLSSYYFDAAGRLPVVLSIHNLDSQMLRSVALGERRFTPAWARAWNR
                     SLAMRAVERRAFPRAYAVVCVSEADAASVNGRARRLLVAPNGVDDAFFSVPPEPPPGE
                     EILFFGQYDYAPNEHGVLRFLRDGWPVLAEARRDARLRLAGKGAGRALEKAVARADRV
                     ELVGVVHDMADELARSRVTIVPIWQGGGTRLKVLESLAAARPVAGTSLGVEGVGFRDG
                     EHGVVADDPAELARRAADLLADATQLPAMGRAGRELAERYRWARALDPATQLYRDLAT
                     A"
     gene            5329..6291
                     /locus_tag="VF752_10700"
     CDS             5329..6291
                     /locus_tag="VF752_10700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856059.1"
                     /translation="MDPPDKRGADVTVVIPVKDRVELLRHTLRSVAEQTQPPAEVVVA
                     DDGSTDRTPEVARELGARVIRKDGGGWGPSAARNAGLAEVRTPYVAFLDSDDLLLPGA
                     LEKLRDALERDLAAPFAYGQAIAAGRNGDDWWFEGVIAARPDELRDPVTSIFVRNHVP
                     SSGALARTEAVRAIGGYDQGLVFDEDHDFWIRLGAHGRPAHVPEVVAIHRRHPGNRGN
                     PLDAIDDELAVVSRADSHDGLVARRAERLGVLLCEVSINAVRRRSLRELAGGVNKLLL
                     RQPERVRILRTSATYFSARRASGRQGERLWQERTDIRDWLSGYR"
     gene            6354..6776
                     /locus_tag="VF752_10705"
     CDS             6354..6776
                     /locus_tag="VF752_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856060.1"
                     /translation="MEKSDVKALAREQLKWSSRTTLKRALGRLPQLVEPGERVVFLGT
                     CSHDGRRGLLAASSRRLLFAGSREEQDTSWSYDGVESIQLSKPSLEPPALVLLAKGKP
                     IAVIDAVAEQSAEIAQFVRDQLWRAILDGRGEAAGPGS"
     gene            complement(6819..8399)
                     /locus_tag="VF752_10710"
     CDS             complement(6819..8399)
                     /locus_tag="VF752_10710"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX8856061.1"
                     /translation="MRSRTVTFFSLLMLTLTPASAQAAGTPAAGHMRVAYDSTPSFTA
                     LSTIAARNQYVILQPWQLSLRSQLKAANPSLKVLAYKNLAFMSSGTGGNGISASGVKY
                     SEADTAHPDWFLHTKAGARFTSWSYSWNWAANVGKADYQQRFGDNVIAELNQNGWDGV
                     FLDDVNPTIKYHHDPNDVLELPNDTAYAAATQSAIQALYPRFRAAGKLMFVNNAAWTE
                     YSSVTTPWLDWVDGMMDEMFTKWGNTTGQGYSSEFDWTKEVAEVKAAEAKGKVFLGVT
                     HSDYTDTAAMRFGWATLLLGANGHSSFQLAHDYTSDSWYPEYDYAIGSPTAAETRDSN
                     GVHRRPYQAGLVLVNPTASTLTVSFGAGTYSGSGLSAATGATMGPHTGLVLTKDGATA
                     PAPAPTPDPGTGTGDTSGGTTSGGTTSGGTTSGGTTSGGTTDISGGTTSGGTTSGGTT
                     SGGTTSGGTTGSTKPGKGRKPKATSARLAVAQRTRRVVRAHKVCVRRHGYRNARCAHY
                     RRVLAKYRRTHAHLRVRS"
     regulatory      complement(8411..8485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1198 a         2997 c         3102 g         1299 t
ORIGIN      
        1 gctcgaggcg atggccgccg gagtgccggt cgtcgcgacg gcggtcggcg ggatcccgga
       61 gatcgcagcc gacggcacgg ccgagctcgt ggcgcccggc gacgacgcgg cgctcgcagc
      121 cgccatcgag cgcctgctcg acgatcgcga cctcgcggcg acgcaggccc gcgcggcgcg
      181 cgagcgggtg ctcgggcgct acaccgccgc ggcgaacgcg cgggcgacgc tcgacctgta
      241 cgagcgcctg ctggagtcgc gccgatgagc gtgctcatga ccgtgtcgga catgagcgtg
      301 ggcggcgccg agcgcatggt ggcccagctc acggtggcgc tgcacgagcg cggccgcgag
      361 acggtcgtgg ctgccgcgcc cgggccgctc gatgccgacc tcgaggcggc ggggatcgag
      421 cgcgagctgc tgccgcgagg gagcaggtcg ccgctgcgcg cggcggcggt cgtgggcgct
      481 ctcgcgcggg tgacggcacg cgtgagcccg gagctcgtgc acgcgcacaa tccgcggata
      541 gcggcgagct cggcgatagc cgctcgcgtg gggcgcccgc tcgcgcgtcc gcccgtgctc
      601 gcgaccttcc acggagtgct gccgtcggag tacggcgtcg cagcgcggct gctccggctc
      661 gccgatcacg tggtgtgcgt gtcggccgag atcgctcaca cgctcgagcg ctctggcgtg
      721 cgtggcgagc gcctctcggt gatcccgaac gccgtggaag ccgtcgagcc gttgaacccc
      781 gagcgccgtg ccgctctcga ctccgagctc gggctcgggg aggcgccggt gttctcgatc
      841 gtcgcgcgcc tggtgccgca gaaggcccat gagcgcttcc tcgaggcagc gcgcatcgtg
      901 gccgagtcgc atcccgagac gcgcttcctc atcgtgggcg acgggccact ccgagcgcag
      961 atggaggggc tcgcgggcga actgaatctc ggcgatcagg tcctcttcac cggcgtacgc
     1021 gccgacgcac gcgacatcat cgcccgctcg cgcgcgctcg tgttctcgtc gaactgggag
     1081 ggcctgtcag tggccgccct cgaggcgctc tccgccggcg taccggtcgt ttcgaccgat
     1141 gtcgagggca tgcgcgagat gctcggctcc ggtgccggag tgatggtcgg ccgtgacccc
     1201 gccgagctcg cggcggcgat cacgcgtctg atcgagcggc ccgacgacgc cgagcagatg
     1261 ggaacgatcg ggcgcgagct tgcgcgcact gtctactccc cgccggcgat ggcggccgcc
     1321 tacgacaacc tgtacgacgg gctgatcgcc gcgcgctagt acgcgagcgc cgcggaccgc
     1381 agcgccgggc cactcgcacg cggcacgagc acgatgcgca cgagcgcgcc tgcgggcgcg
     1441 gtcacgcgga cggtcgcagc gctccgcggg cgcttcacgc gcgttatgcg ccgccagtgc
     1501 gcccggccgt gcgcggtcac gagccgctcg gcgtactgcg cgcggaacag acgcggcgcg
     1561 taggccgcca gccgcgtctg cacgccgcgt gacaccgcgc caccggcgga cgcctgggcg
     1621 gcggctgccg aggcgatgtg gacggccacc gcgcgtgacg ctcgcagcat ccgattgctc
     1681 ctcgtaaccg cggccacgga gtagcggtgg gtcgcgcccg gaaccggggc gcggtcgatc
     1741 cagcggcgcc cgtgggttgt gcccaccagc cggccgtcgc ggaacacgcg gtagcggccg
     1801 atgcgccgcc cgctcttcga gcgcgtccag gtgacgctca cgcttccggg cgaggccgag
     1861 gtcgcgagca cagagagccg cgagctcgcc cgacgcgcgg acggcgagga cctgggcgac
     1921 cggcccttgg tcgggctcgg cgatccggcg ttgccaccgt cgcgcgtgag gatcagcgcg
     1981 cagtgggcgc ccagcgtcgc acccgtggct ccggagagcc cggagccgga gtagctgccg
     2041 ccgaacgaca cgctcttgtc gctcgcggtc gggttcacga cgacgaggcc gttcgtgaag
     2101 gttcgacggt gcacgccgtc cgcgatgcgc gactcagcgc cggtcggctc gccgatcgcg
     2161 tagtcgaact ccgggaacca gtactcgttc gtgtagtcgc tcgccacctg gaagtacgag
     2221 tggccggcgc ccgcgagtag cgtagtcgcc cagccgaagc gggcggcggc acggtcgctc
     2281 gccgggccgt gcgcgacacc gaggaacagc ttgttcttcg cctcagcgtc cttaacctgc
     2341 gcgagctcgc gctcccactg ccactggtcg gagtagccct cgccggccct gttgccccac
     2401 ttcgtgaaca tctcgtcgag catgccgctg agatcgtcga gccacggcgt ggtcacgctc
     2461 gagtactccg tccacgaggc cacgttcgcg accacgagct tgccggcggc ctggatgcgc
     2521 gggcccacgt agccgatcgc gtcggacatc gcgcggccgt atgacgcatc ccccgagtac
     2581 tcctgcacgt cgcctgggtc gtgctggaac ttgatcgtgg gcgagacgtc gtcgagcatc
     2641 acgccgtcga agccggtctc cttcagttcg gcgattacgt tgtcggcgaa ggcctgctga
     2701 taggtgggat cgccgatgtc ggccgcccag agccacttcc atgtccacga cgcgaagcgc
     2761 ttgcccgctt tgtcgtgcag gaaccatccc tcgtgcgcct gcgcctgcgg ccaggtgacg
     2821 cccgacgcgg agatgccgct gtaggaagcg gtctcgctga tgaagccgag gttcttgtac
     2881 gcgagcacct tgatgccggg gtcggaggcc ttcaactgtc ccagcagacc cgtctgccac
     2941 ggctgcagga tcacgacggc gctgcgcgcg gtgttctgcg acgacgccgc gatcgaggga
     3001 ttcgagtcga tcgccacgcg gacgtgcccg gctaccgccg cctgcgcgcc cgcgggcagc
     3061 gcgagggcga gcgccgtggc gatgacggtt gggagggctc tgatgcgcaa gcgcggtgtc
     3121 ctttcggcag ctgggccgtc tcggggagac ccctggcttt gcgaccccgc ctcgcggcgg
     3181 gtgtgccttt ctcgaggagc acgcggcgcg ctccccctgc atcagttgtg ctcggagatt
     3241 ctgcccagcc gcttggagcg cctagcggca tctggacgtt tgttctaggt ctggcgctta
     3301 ggccctaacg gctgccaggc ggcaaccgag gaccttgcgc gcaagcggga ggcgcgcgat
     3361 gcgccggccc ggtccgccga ggcgctcgag cagcgtctcg aacgcggtca cgaacgcgac
     3421 ggggaagcgc aggtgcagcg gtcccacctg cacgaacgcc tgcacccgct cgaaccctgt
     3481 ctcgcgcagg agcgcgacga gctcccggtt cgtgtactca cgcaggtgga agccggtggg
     3541 ctcgtcgtcg aagtgcttgg agatgtcgtg cggcccggac aggcggttcg gcgtgatgaa
     3601 cacgtagcga ccaccggcgc ggagcgcccc gtggatctgg ccgagctgct cacgcgcgtc
     3661 gtccgggtgc aggtgctcca tgagctgatc ggagaagacg acgtcgaccg agccgggcgc
     3721 gaccggcacg ctgcgcccgt ccgagatgat cagctcgaag ttcgccgggt tgccctctct
     3781 tggagcgatc tccgccgaca cgtcgatcgc atacacgcga gcgacacgcc gggcgacctc
     3841 gaacgcgagc gcgcagtcgc ccgccccgat ctcgaggtag gtgttctcgc gagaaaggaa
     3901 gggcttcagc agcgcgagct cctcgcgcat gcgctccgcc tgcgccgcgg cactcgcctt
     3961 gcgctcgttc tgcggatggt ccggaacgcg ccggaagagc tcgtcgtaga cctcggagta
     4021 gaggcgcgct cgttcttgcc agtccgagtt gcggagccgg tcggagagct cccgctcaat
     4081 ctcgtagtgg cgacggatcc cctccggctg cctgcgcatc gcgggcatct taggcggtgg
     4141 ccagatcgcg atagagctgc gtggcggggt cgagcgcgcg cgcccagcgg tagcgctcgg
     4201 ccagctcgcg tccggcccgc cccatcgcgg gcagttgcgt cgcgtcggcc agaaggtccg
     4261 cggcgcgccg cgcaagctct gcgggatcgt ccgccacaac accgtgttcg ccatcgcgga
     4321 agccgactcc ttccactccg agcgacgtgc cggcgaccgg ccgcgccgcc gcgagcgact
     4381 cgagcacctt cagcctggtg ccgccgccct gccagatcgg gacgatcgtg acgcggctgc
     4441 gtgctagctc gtccgccatg tcgtgaacga cgcccacgag ctccacgcgg tcggcgcgcg
     4501 cgaccgcctt ctcgagcgcg cgccccgccc ccttgccggc aagccgcagc cgcgcgtcgc
     4561 gccgcgcctc ggccagcacc ggccatccgt cgcgaaggaa gcgcagcacg ccgtgctcgt
     4621 tcggggcgta gtcgtactgg ccgaagaaga ggatctcctc gccgggcggc ggctccgggg
     4681 gcacggagaa gaacgcgtca tccacgccgt tcggagcgac aagcaggcgg cgcgcgcgcc
     4741 cattcacgct tgcggcgtcc gcttcggaga cgcacaccac ggcatacgcg cgcgggaatg
     4801 cgcgccgctc gaccgcgcgc atcgcgagtg agcggttcca tgcccgcgcc cacgccggcg
     4861 tgaaccggcg ctctccgagc gcgacgctac gaagcatctg cgagtccagg ttgtggatgc
     4921 tcagcaccac gggcaggcgt ccggccgcgt cgaagtagta gctcgagagt gtgtgctcga
     4981 gctgcacgaa cgcggcccct tcggtggaga gctcgcgcag gcgatggtct acggaatcga
     5041 ggaacgggct ggggcgccgg tgcgcgtgct ggctcagccg cgacacgatc gtgccggact
     5101 cgccgggaag cagctcgacg tcgaagccga gatctcgcag cgcgtcgatg gccccaggct
     5161 gcccgggatc agtggtgagc acgtgaggtt gcactcccgc ccgctgcatc gactcgagga
     5221 gccgaagcgt gcgcttgtag ccgccgccga ccggcggata cggcacaacg gggcacacga
     5281 cgagcgcgcg cggttttgcg atctccccca ctacctagag tgtcgccgat ggatccgccg
     5341 gacaagcggg gcgccgacgt gacggtggtc atccccgtca aggacagggt cgagctgctc
     5401 cgccacacgc tgcgcagcgt ggccgagcag acgcagccgc ccgccgaggt ggtggtcgcg
     5461 gacgacggct cgaccgaccg cacgcccgag gtggcgcgcg agctcggcgc gcgcgtgatc
     5521 cgaaaggacg gcggcggatg ggggccgtcc gccgctcgca acgcagggct cgccgaggtg
     5581 cgcactccgt acgtcgcgtt cctcgactcg gacgacctgc tgctgccggg agctctcgag
     5641 aagcttcgcg acgccctcga gcgagacctg gcagcgccgt tcgcctacgg gcaggccatc
     5701 gcggcgggcc gcaacggcga cgactggtgg ttcgagggcg tgatcgcggc acgccccgat
     5761 gagctccggg accctgtgac atcgatcttc gtccgcaacc acgtgccctc atcaggcgcg
     5821 ctcgcccgga ccgaagccgt ccgcgcgatc ggcggctacg accaggggct ggtcttcgac
     5881 gaggaccacg acttctggat ccggctcggc gcccatggcc ggccggcgca cgtccccgag
     5941 gtcgtggcga tccaccggcg ccatccgggc aaccgcggca acccgctcga cgccatcgac
     6001 gacgagctcg cggtcgtgtc gcgtgccgac tcgcacgacg gtctcgtcgc acgccgcgcc
     6061 gagaggctcg gtgtgctgct gtgcgaggtc tcgatcaacg ctgtccgccg gcgcagcctc
     6121 cgggaactcg ccggcggggt caacaagctg ctccttcgcc agcccgaacg cgtgcgaatc
     6181 ttgcgcacgt ccgccaccta cttttcagct cggcgtgctt ccggccgaca aggtgagcgg
     6241 ctgtggcaag agcgcaccga catccgggat tggctgagcg gctatcgctg aatagccctg
     6301 ccgctggcat gcttgatccc gaggcgcgcc gggaaaccgc ctcgcggagg gcgatggaga
     6361 agtccgacgt caaagcgctc gcgcgcgagc agctgaagtg gagcagccgc acgacgctca
     6421 agcgcgcgct cggccgtctt ccccagctcg tcgaaccggg cgagcgcgtt gtcttcctgg
     6481 gcacctgctc acacgacggc cgccgcggcc tgctcgcagc gtcgagccgg cggctgctgt
     6541 tcgcaggcag ccgcgaggag caggacacga gctggagcta cgacggcgtc gaatcgatcc
     6601 agctcagcaa gccctccctc gagccgcccg cgctcgtgct gctcgcgaag ggcaagccca
     6661 tcgcggtcat cgacgcggtg gccgagcagt ccgccgagat cgcgcaattc gtgcgcgacc
     6721 agctctggcg cgcgatcctc gacggccgag gggaggccgc cgggcccggc agctagccga
     6781 gcccggcgtc ccctgagggt tccgtctgag ggttccgtct aggaccggac gcgcagatgc
     6841 gcgtgcgtcc ggcggtattt ggccaacacg cggcggtagt gcgcgcagcg tgcgttgcgg
     6901 tagccgtgcc ggcgaacgca caccttgtgt gcgcgtacga cccggcgggt ccgttgtgcg
     6961 accgccaggc gagccgaggt cgccttgggc ttgcgaccct tgcccggctt ggtggagccg
     7021 gtggtcccgc cggacgtcgt gcccccggag gtcgtcccgc cggaggtcgt gccgccggac
     7081 gtggtgccgc ccgaaatatc ggtcgtgccg ccggacgtgg tgccgcccga cgtcgtgccg
     7141 cccgaggtgg tgccaccgga cgtcgtgccg ccggaggtgt caccggtgcc ggtccccgga
     7201 tcgggcgtag gagcgggcgc cggcgcagtc gcgccgtcct tcgtcagcac cagcccggtg
     7261 tgcggcccca tggtggcgcc ggtcgccgcg ctcagccccg acccggagta ggtgccggca
     7321 ccgaaggaca cggtgagcgt cgaggcggtg gggttcacca gcacgagacc ggcctggtac
     7381 ggacgacgat gtacgccgtt cgagtcgcgg gtctctgccg cggtcggcga gccgatcgcg
     7441 tagtcgtact cggggtacca gctgtcgctc gtgtaatcgt gggcgagctg gaaggagctg
     7501 tgcccgttcg cgccgagcag caacgtcgcc cagccgaagc gcatggcagc ggtgtccgtg
     7561 tagtcggagt gcgtgacgcc gaggaacacc ttgcccttgg cctccgccgc cttgacctcg
     7621 gcgacctcct tcgtccagtc gaactcgctg gagtagccct ggccggtggt gttcccccac
     7681 ttggtgaaca tctcgtccat catcccgtcg acccagtcga gccacggagt cgtgacgctc
     7741 gagtactcgg tccacgccgc gttgttcacg aacatcagct tcccggccgc ccggaagcgc
     7801 gggtagagcg cctggatcgc agactgggtg gccgccgcat aggcggtgtc gttgggcagt
     7861 tcgagcacgt cgttcgggtc gtggtggtac ttgatcgtgg ggttgacgtc gtcgaggaag
     7921 acgccgtccc agccgttctg gttcagctca gcgatcacgt tgtcgccgaa gcgctgctgg
     7981 tagtccgcct tgccgacgtt cgcggcccag ttccagctgt aactccacga ggtgaagcgc
     8041 gcgcccgcct tggtgtggag gaaccaatcc gggtgcgcgg tgtcagcctc ggagtacttc
     8101 acgccgctcg ccgagatgcc gtttcccccg gtgccgctcg acatgaaggc gaggttcttg
     8161 tacgcgagca ccttgaggct cgggttggcg gccttcaact ggctgcgaag cgagagctgc
     8221 cacggctgga ggatcacgta ctggttgcgg gcggcgatgg tgctgagtgc cgtgaaggag
     8281 ggtgtcgagt cgtacgccac gcgcatgtgc ccagctgccg gcgtgcctgc cgcctgcgcc
     8341 gacgccggcg tgagcgtgag catgagcagt gagaagaatg tgacggtccg ggaccgcatg
     8401 acatcttcct ttcgggagct gggctgtctg cgagagaccc ctggctttgc gaccccgcct
     8461 cgcgacgggt gtgcctttgt cgtttgaagg gcacgcggcc cccaggcttt cgccagcgtg
     8521 atcggcgcac cggttgcccc acgggcacct ggtccggcgg catttggacg gctgtacgaa
     8581 agtcctggac ggaggt
//
LOCUS       DASVXS010000195        20206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1087070, whole genome shotgun sequence.
ACCESSION   DASVXS010000195 DASVXS010000000
VERSION     DASVXS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 20206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20206
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087070"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF752_10715"
     CDS             <1..413
                     /locus_tag="VF752_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8856062.1"
                     /translation="WGDQLDGFLARVTGQYSRLGALLDPLTDRLLVVSGVIVCWKFEL
                     LPRWGLAVLVARELVMLVLTQAALRRGMDLDIKMVGRWAVWPVMSAIGLSLIFGPNWV
                     ANVSLYVGIALTIWATVLYMRDGLHELRKHSSSA"
     gene            460..729
                     /locus_tag="VF752_10720"
     CDS             460..729
                     /locus_tag="VF752_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856063.1"
                     /translation="METFPDLGALSDQELKDLIEELTEEEQELSYRRRILHGKIDILR
                     AELVNRLRKKHEEGEPVITGADVQQLTDILAGKSIPQGGNEEPSQ"
     gene            739..1197
                     /locus_tag="VF752_10725"
     CDS             739..1197
                     /locus_tag="VF752_10725"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8856064.1"
                     /translation="MHCPECGFVNAEGANYCQKCGAFLGDKVADDTTVAYQLDNETGE
                     LRPVDVDPVVTEGASLVIRSGGGRSGESFDVKGERLAIGRSPEAEVFLDDVTVSRNHA
                     LLVRRRDGVYIDDLGSLNGTYVNRRRIESHKLVDGDELQIGKYKLTYLER"
     gene            1206..1991
                     /locus_tag="VF752_10730"
     CDS             1206..1991
                     /locus_tag="VF752_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8856065.1"
                     /translation="MEQKLAPVRRSGENGRESAEALRERAGAPPVGKSLTIGAVCKQL
                     EREFPDISISKIRYLEDQKLLSPRRTPGGYRLYSPGDVSRLRTILRLQRDEFLPLRVI
                     RQELAAGRADSDAGVPSGGPDPRTLRRTRVADQGGALYTLADVLDDTGAEAKLVRELE
                     EYGVVKPEKRDGEVLYDDTSREIIRAVKELARFGVGGRNLRVFRTSADREAALLEQIL
                     APALRSRNPERRKEAVEALENLAATVSHLKYLLLARDLRKFTQ"
     gene            1988..2536
                     /locus_tag="VF752_10735"
     CDS             1988..2536
                     /locus_tag="VF752_10735"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX8856066.1"
                     /translation="MTGAQRAGEADLRKYIYEIPDFPRKGIVFKDVTPLLLNPDATRR
                     AVNQLAGYASRLEVDYVVSAEARGFILGGAVAAQIGAGFIPARKPGKLPRETTSVEYV
                     LEYGIDALEVHRDALAGGARVLVHDDLLATGGTARALCDLVEKAGAQVQGCAFIIELA
                     FLNGRERLAPYDVHSLITYEDE"
     gene            2539..2982
                     /locus_tag="VF752_10740"
     CDS             2539..2982
                     /locus_tag="VF752_10740"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8856067.1"
                     /translation="MPTVRRARTIAARPEDIWPLIADPHHLPRWWPDLARVEEATPKA
                     WTKVLQSRSGRGVRADFSRVSANRPRRLVWRQELVDSPFERILAEAVTEIQLEPAEGG
                     TRVEIAVTEKPKGMNRFGGFMFRLAARKRIDRALEGLDEIAGDRT"
     gene            2982..4550
                     /locus_tag="VF752_10745"
     CDS             2982..4550
                     /locus_tag="VF752_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8856068.1"
                     /translation="MRWWGWGEDRDAIHLPPHAVDMLREQLGIDGSRRRHVPLEEVSL
                     PEPAEVRLPGEVRTDREARVLHAVGRGYPDLVRIRSGDGSSAPDAVAYPASHDEVAEL
                     LRACVEQNVAVVPFGGGTSVVGGVEPLRDGHSAVVTFDLSRLDKLISVDRVSLLATFA
                     PGLTGPRAEALLASRGLTLGHYPQSFEYATVGGFVATRSAGQASTGYGRIDELVRGVR
                     LAAPAGDLAVKTVPASAAGPSLRELVVGSEGALGVITEATFMVRPLPRVRRYEGWSFR
                     SFAEGCEAFRTLEHEDSSPDVARLSDEEETRLNMALASSGSLADRAGRAYLRARGHEG
                     GCIVIVGYEGSERDVALKRGFASAILKRSGGLPLGTSPGRSWLRNRYAAPYLRDELLD
                     LGVMVETLETATTWSNLLNLHRAVREALHAHAPLVMCHVSHLYRSGASLYFTFLGRQD
                     PGREIDQWRAAKAAASDAIVANGGTITHHHAIGRDHLPWMPAEVGEPGLELLRAAKER
                     LDPAGIMNPGKLIP"
     gene            4787..5626
                     /locus_tag="VF752_10750"
     CDS             4787..5626
                     /locus_tag="VF752_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856069.1"
                     /translation="MALTQRETQQVEQANASGRTPVVFIHGLWLLPSSWDNWVSLFQE
                     AGYAPLTPDWPDDPETVEQARANPDVLAKKTLGQVAAHTSEVIGKLDKKAAVMGHSTG
                     GLLAQMIAGRGLSAATVAIDPGPFRGVLPLPFSTLKVTLPVLKNPLNKNRAITLTLEQ
                     FKYGWVNALSDEEGRRLYETYHVAGPGVALMQMANANLNPRTEARVDTKNPNRGPLLI
                     LDGEKDHTVPWAIANAAFKRQQQNPGVTEIEKIPNRGHSLTIDSGWREVADKALAFVK
                     RFV"
     gene            complement(5639..6685)
                     /locus_tag="VF752_10755"
     CDS             complement(5639..6685)
                     /locus_tag="VF752_10755"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX8856070.1"
                     /translation="MATFDRLSAEQRAIIELVLQRGQTYEELSGMLGMPASRVRDLAR
                     EAMGELAPRTADRVDPDWRGQIADYMLGQQTGPESTATRGHLKRSEPARAWALSLLDS
                     LEDLYPNGEAPEIPEAERNGDRPARRDLGGRLTAAVESPERATARTAPRPRRDRSLRG
                     GVADTVKRRRLIAAVIGGLVVLGLILWLTGVFDSGGGKKNTNAAAPKTKLVKVGTLKA
                     TGGSKAAGVAAVFTRGNQRSLVVQAAQLQPTGQKNAYEVWLYNSKSDAFAVCAQLTDQ
                     NGNLQGTGPLPANYTKFGSIVLSREKIGTNPKSPSNIVLRGKLTNVPANAQTGAANAL
                     GGSSTTPSGTTTTP"
     gene            6714..7169
                     /locus_tag="VF752_10760"
     CDS             6714..7169
                     /locus_tag="VF752_10760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8856071.1"
                     /translation="MSARKNSVREAEEAYGIPWERTLDGTLGFEIVEIGDGTARARFD
                     VTDRVRQPFGLVHGGAYSSLAESLSSAATFQAVADGGNIAMGSSNNTTFFRPVTEGSI
                     NAEATARHRGRTQWVWDVEFTDDQGRLTAASRVTIAVRPRPASPPRSGS"
     gene            complement(7117..8874)
                     /locus_tag="VF752_10765"
     CDS             complement(7117..8874)
                     /locus_tag="VF752_10765"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX8856072.1"
                     /translation="MRVALAQMNSTVGDIGGNAARIREKIEAARDEGAALVVLPELVL
                     TGYPPEDLLLKRNFLERASAALESIAESAQGIVAVVGFPELDKDVYNAAAVLADGRVA
                     GVYRKNYLPNYGVFDERRYFQSSRDAALIEVNGVLIGITICEDIWEPGPPLTAEALAG
                     AHLIVNLSASPYHAGKGAEREQMLVQRARDSIVAIAFCNMVGGQDELVFDGYSLGIDG
                     DGEVIARAPQFEESMCTFTFDPSAVETGRLRDTRNRAAAQRSWEEGSAEPTVLARLEV
                     SGSDTEHDVGGPVAPPLSQEEEIYRALVTGVRDYVEKNGFSEVVFGLSGGIDSALVAL
                     VAADALGPDRVNAVIMPSPYSSDETQADARTIAQNVGAEVFELPIEQPMQAFDSMLSD
                     VFAGREPDITEENLQARIRGNLVMALSNKFGWLVLTTGNKSELSVGYATLYGDMAGGF
                     AVLKDVFKHTVYDLVRWRNANEGRELVPATVLERPPSAELRYEQRDSDSLPPYDVLDG
                     ILKGYIEEELDAEELERRGLPGDEVERVIRMVDRAEYKRRQAPPGIRISVKAFGRDRR
                     LPITNRFEAGSRAAAEPRS"
     gene            complement(8935..9342)
                     /locus_tag="VF752_10770"
     CDS             complement(8935..9342)
                     /locus_tag="VF752_10770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8856073.1"
                     /translation="MAGKHAHETLQIRPDPAELARTREFVRGRAAACGLDEEARFRAA
                     LVVTEAVTNAMRHGRGSDAAEPIEVRCGCNGEGFLIEVGDCGRFRRRNRAPRGATHGR
                     GLGLIERLTRRFDLEASTEGTRLRMLVGDQNRI"
     gene            complement(9473..9841)
                     /locus_tag="VF752_10775"
     CDS             complement(9473..9841)
                     /locus_tag="VF752_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8856074.1"
                     /translation="MKKIEAIIRPERLQEVQDALDELGVSGLTVSEVMGCGRQKGYTE
                     QYRGTRANISLLPKVKVESVVPDDVADRAVEAIVGGARTGEIGDGRVFVYPVEQSVRI
                     RTDERGEDSVRHEAPVGWGH"
     gene            complement(9838..11226)
                     /locus_tag="VF752_10780"
     CDS             complement(9838..11226)
                     /locus_tag="VF752_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8856075.1"
                     /translation="MAPTIKDAVPAIETLWVVVAAVLVFFMQAGFLLLEMGFSRMKNA
                     GTVVPKVLANLSIAGLCYWAVGFALAFGGSGWLAGTHGTFLGTTNGDLFPAMAFSSAA
                     VPAKFFFQFVFCAVSLAIVWGTTLERIKFGAYLIYAVVFSALIYPVVSHWVFGGGWLQ
                     ANFGMQDFAGSTAVHLIGATGALAALLLLGARQGKYGRDGKPNVIPGHSMPFVGLAVL
                     ILWFGWFGFNPGSTLGAIGGRFADVAVVTNLAAAAGVLGAMGTIYALTRTFDIGMTAN
                     GAIAALVAITAPSGYVEFWAAPIVGFVAGVIVVFSVILIDRKLDDPVGALSAHGMAGV
                     WGTLSCGLFTAPRLAELNGVGQGGLFYTGSIHQLAVQALGVVSAFTAVFVLSYLVFWA
                     IKSTYGLRVKPEDERYGLDLVEHGMWGYPEQFMPVPGTEYHPAAGSPLRPGVGPLPAG
                     LHAMTSREAGTR"
     gene            complement(11371..11814)
                     /locus_tag="VF752_10785"
     CDS             complement(11371..11814)
                     /locus_tag="VF752_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX8856076.1"
                     /translation="MSTARDLGLMVSMKRLERPTRTVAEAARAVGTEPARIAKSIVFV
                     ADGEPMVCVASGAHKIDKRALCDVLDCAVLHAASPDEVRAATGFSPGGVPPFSHGLPV
                     VIDEALLEFPTVWAAGGDGNTVFEVDPKVLASCCKATVAAVGDES"
     gene            complement(11900..13351)
                     /locus_tag="VF752_10790"
     CDS             complement(11900..13351)
                     /locus_tag="VF752_10790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8856077.1"
                     /translation="MAGDRLTGLDASFLHMERDGAHMHVAGLMLFDGPPPAYEELIEA
                     IERRLHLVPRYRQRLAFVPLGQGRPTWVDDPHLNLGYHVRRTALPPPGSEDELRTLAG
                     RVFSQPLDRDKPLWEMWLVEGLADGGFAILSKTHHALVDGVSGVDIASVLFDTSPEPA
                     EPPNPPPRWVARPLPSHADLLAEALLERATLPTEMVRTARATLRGPRAALRAAGTALG
                     GVGAMAGAGLNPAPSSPYNVKLGPHRRFRWVRASLDDVKAIKNALGGTVNDVVLTVVS
                     QALGRHLRGRGVDTDGLVLKAMVPVSVRAQSERGALGNKVAAMMAPLPVGVEDPLEAM
                     RQISDAMADLKSSGQAVGAQVLTELTGFAPPTIMSQAARLTARQRFFNLVVTNVPGPQ
                     FPLYLLGRELIDIFPMVPLAQGQALGVAIMSYNGRLNIGLNADWDAMSDIEDLVADFE
                     GSFAALERAAELAPARPSRRPAPAPVRRFQPTE"
     gene            13440..14840
                     /locus_tag="VF752_10795"
     CDS             13440..14840
                     /locus_tag="VF752_10795"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8856078.1"
                     /translation="MSTDARLIRQAYRFALAPSPRQEAFLASCCGTSRFWFNQGLALV
                     KDRLDRRGAGEHARVPWSYKSLCSEFAPLKDEVCPWRSEVVVGSMQAGLEQLGRALQK
                     YSKTRAAGRPVGFPRFRAKGRCHESVIFQRPRIADSRHVLLDRRLGPMRTKEPMRKLL
                     RLLADDERARVLRSTVKRTPRGWTISFTVERSPKRRHARRPTAAVGVDLGLTRLATLS
                     TGEAAANSRPLRAVLVKLRRLQRQLDRQRRAANARNYLADGRVRAGAKTWSKSMRMGD
                     TEKRIKRLHARVANLRREQAHQLTTYLTREFGVIGVETLAVSNMIRNRRLARHIGDVG
                     WGIILTQLKYKTSWSDGSLLIAANRFHPSSRTCSACGAVKAKLSLNERVFTCDGPACG
                     HVQDRDLNAALNLACLAARHAQAEGLQCHVAATGAETLNARRGQVRLDPVEHSPAKRE
                     ESSDSSQHGDALALAV"
     gene            14895..16052
                     /locus_tag="VF752_10800"
     CDS             14895..16052
                     /locus_tag="VF752_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8856079.1"
                     /translation="MRTDELLRPWYDAAAAMVPGLRTFDVHTHTGHADPDEFHQTPEE
                     LLTGLSAVDGRAVVFTLHEPDGYSEPNDRVRAEAAASGGKLVPFCRVNPHADALAEAR
                     RSLDAGARGIKLHPRAERFTLDHPTVGDLFELAQERSVPIIVHAGRGIPALGRHTLEQ
                     AERHPGANVILAHAGICDLAWLWRHGEERPNLLYDSAWWTATDLIALFSLVPPGRILF
                     GSDSPYGTPMHESILTLRCARQAGLSDDQVREIMGGQVERLLAGEPLADLGPAPMKPA
                     EADLLLDRAYTFLIQAIIACFGRVMDRVGESLALVRLCCEVGEDAPQAEACRSVLSLV
                     DRFEHYQAVRDDDPRDFAGVSLLIVAAVVARTPNVPLPETPEPVSVSERTD"
     gene            16380..17072
                     /locus_tag="VF752_10805"
     CDS             16380..17072
                     /locus_tag="VF752_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX8856080.1"
                     /translation="MKSILAARVRSARRAAPIAAAALVLGAAGGGAAIAEAAGTTGAS
                     TAAPTPHVHAKFTREQLAGHPVRARGVAHPSDRTHTVLMQARRGHGRWQTVDRARTQA
                     GHFRASWRPSSTGRYQVRAVLAGATGAAGAKPAQRTVRVYRAAAASWYGPGFYGHRTA
                     CGHTLTPGTLGVANKSLPCGTRVTFRYGRYRVTVPVIDRGPYAGARVWDLTPATKSRL
                     HFPSTGVVWSTR"
     gene            complement(17277..18332)
                     /gene="dinB"
                     /locus_tag="VF752_10810"
     CDS             complement(17277..18332)
                     /gene="dinB"
                     /locus_tag="VF752_10810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8856081.1"
                     /translation="MERCIAHVDMDAFYVSVELQRRPELIGKPVVVAGSSPRAVVTTA
                     SYEARKFGVQSATPAEWARRRCPDAVFIPPDFEHYRARSREVMEVLRAQVETVEIVGL
                     DEAYLDLTGIDRPRAAARRIKEAVREATGLVCSIGVGPSKLVAKVASDAEKPDGFVFL
                     SREEACARFAAASPGLVPGIGPKTVKRLEENDIRTLGDLAAAPTEQLVELFGPRLGPH
                     LSNLARFEDDRLLETDRVAKSESRETTFDRDLRRLDEMEPVVVKLADELCAGLVRAGQ
                     SGRTIGIKVRLDDFSIHTRARSLAEPTNDSAAVTRIALALLRDFDPPRPVRLLGVRVA
                     GLDEGSSVGDDQLALAL"
     gene            complement(18377..19339)
                     /locus_tag="VF752_10815"
     CDS             complement(18377..19339)
                     /locus_tag="VF752_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8856082.1"
                     /translation="MGKPILVGYDPRTIDNAPLSFGIAAARFTGARLIVACVTSEKVP
                     SLPVSGEPLDYAVGQTDADLTHDASEAARAVQQHLEHEGIAYEVRLLQSSSAAKALHR
                     EADDDDAGLLVIGSSRRSGVGRALAGGTAVRLLHGAPCPVTVVPRAWTRGGPPSVIGV
                     AYVDTEEAREALRGAYALARRAGATLRVITVVKVTASMHFETEPATASQAGKSLEEVE
                     GEHRLEAERRLRVAVGELGDDVSVEVEAVIGDPGEALVELSNGVDLLVCGSRGYGPAR
                     AVLLGSVSRHVVSEAHCPVIVLPRGVKASLEALVTEESGAGAPA"
     gene            complement(19375..20070)
                     /locus_tag="VF752_10820"
     CDS             complement(19375..20070)
                     /locus_tag="VF752_10820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8856083.1"
                     /translation="MADERPVIVITGASSGIGAATARHAAEAGWRVVLGARSEDRLAA
                     LAAELGGDEVAIGRRCDVTDWNDQQALAAAALDHFGRLDAMFANAGFGAKRGFLEETP
                     EFWRSMIDTNVYGTALSIRASLAHFRNEGRGHVVLTSSVAGRRALPGSLYSATKWAVT
                     AMGEGLRQEVSDSDIRVTLIEPGAVDTPFFDNRPEVALEADDIARAVVFALSQPAHVD
                     VNEILIRPVRQPS"
BASE COUNT         3127 a         7022 c         7020 g         3037 t
ORIGIN      
        1 cgtggggcga ccagctcgac ggcttcctcg cgcgcgtgac gggccagtac agccggctcg
       61 gtgcgctgct cgacccgctc acggaccggt tgctggtggt gtcgggcgtg atcgtctgct
      121 ggaagttcga gcttctgccg cgctgggggc tcgccgtcct cgtcgcgcgc gagctggtga
      181 tgctggtgct cacgcaggcg gcgctccgcc gcggaatgga cctcgacatc aaaatggtcg
      241 ggcgctgggc ggtctggcct gtgatgagcg cgatcgggct ctcgctgatc ttcggcccta
      301 actgggtggc caacgtatcc ctgtatgtcg ggatagctct gaccatctgg gcgacggtgc
      361 tctacatgag agacggcttg cacgaactgc gcaaacactc aagctctgct tgaacgtctg
      421 gtctatactc gccgctccgc catccacaca gggggagcca tggagacctt tcccgatctc
      481 ggcgcgctgt cggatcagga actgaaggat ctgatcgagg aactcacgga agaggagcag
      541 gagctctcct accggcggag gatcctccac ggcaagatcg acattctgcg cgctgagctg
      601 gtgaaccggc tcaggaaaaa gcacgaggag ggcgagcccg tgatcacagg cgccgacgtt
      661 cagcagctca ccgacatcct cgccggtaag agcatccccc aaggcggaaa cgaagagccg
      721 agccagtagg gcagcgccat gcactgtccg gaatgcggct tcgtcaacgc cgagggcgcc
      781 aactactgcc agaagtgcgg cgccttcctc ggcgacaagg tggctgacga caccaccgtc
      841 gcctaccagc tcgacaacga gacgggcgaa ctgcgcccgg tcgatgtcga tcccgtggtc
      901 acggagggcg cgtcactagt gatccggtcc ggcggcggcc gttccggaga gagcttcgac
      961 gtgaaggggg agaggctcgc catcggccgc agccccgagg cggaggtgtt cctcgacgac
     1021 gtgaccgtgt cccgcaacca cgcgctgctc gtgcgccggc gcgacggcgt gtacatcgac
     1081 gatctcggct cgctgaacgg gacgtatgtg aaccgcaggc ggatcgagtc gcacaagctc
     1141 gtcgacggcg acgagctgca gatcggcaag tacaagctca cctacctgga gagatgagcc
     1201 gcgatatgga acagaagctt gcgccagtac ggagaagcgg cgagaacggg cgcgaaagcg
     1261 ccgaggccct acgcgagcgc gcgggcgcgc cgccggtcgg caagtcactc acgatcggcg
     1321 ccgtctgcaa gcagctcgag cgtgaattcc ccgacatctc gatctccaag atccgctacc
     1381 tggaggacca gaagctgctg tcgccgcggc gcacccccgg gggctaccgc ctctactcgc
     1441 ccggcgacgt gtcgcggctg cgcacgatcc tgaggctgca gcgcgacgag ttcctgccgc
     1501 tgcgggtgat ccgccaagag ctcgccgccg gtcgggcgga ctccgacgcc ggcgtgccga
     1561 gcggcggccc cgacccgcgc actctgcggc gcacgcgcgt ggccgaccag ggcggcgcgc
     1621 tctacacgct cgccgacgtg ctcgacgaca ccggcgccga ggccaagctc gtgcgcgagc
     1681 tcgaggagta cggcgtggtg aagccggaga agcgcgacgg cgaggtgctc tacgacgaca
     1741 cctcgcgcga gatcatccgc gcggtgaagg agctggcgcg cttcggggtc ggcggtcgca
     1801 acctgcgcgt gttccgcacc tccgcggacc gcgaagcggc gctgctcgag cagatcctcg
     1861 cgccggcgct gcggtcccgg aacccggagc gccgcaagga ggcagtcgag gcgctcgaga
     1921 acctcgcggc gacggtctcg cacctgaagt atttgctcct cgcgagagac cttcgtaaat
     1981 ttacgcagtg acgggcgctc agcgtgcggg tgaagccgac ctgcgcaagt acatctacga
     2041 gatcccggac ttcccgcgga agggcatcgt cttcaaggac gtgacgccgc tcctgctcaa
     2101 ccccgacgcg acgcggcggg cggtcaacca actggcgggg tacgcgagcc ggctggaggt
     2161 ggactacgtg gtgtccgccg aagcacgggg gttcatcctc ggtggtgcgg ttgccgcgca
     2221 gatcggcgcc ggcttcatcc cggcccgcaa gccggggaag ctgccgcgcg agaccacttc
     2281 cgtggagtac gtgctcgagt acggcatcga cgcgctcgag gtgcatcgcg acgcgctcgc
     2341 cggcggagcg cgcgtgctgg tgcacgacga tctcctggcg accggcggca ctgcgcgggc
     2401 gctctgcgac ctggtcgaga aggccggcgc ccaggtgcag ggctgcgcct tcatcatcga
     2461 gctcgccttc ctgaacgggc gcgagcggct cgcgccctac gacgtccaca gcctcatcac
     2521 ctacgaggac gagtagcggt gcccacggtc cggcgcgccc gcacgatcgc ggcgcggccc
     2581 gaggacatct ggccgcttat cgccgatccg catcacctgc cgcgctggtg gccggacctg
     2641 gcgcgcgtgg aagaggcgac gcccaaggcc tggacgaagg tgctccagtc gcgcagcggg
     2701 cgcggggtcc gcgcggactt ctcgcgtgtg tccgcgaacc ggccgcggcg gctcgtgtgg
     2761 cgccaggagc tcgtggactc gccgttcgag cggatcctcg cggaggcggt caccgagatt
     2821 cagctcgagc cggccgaggg cggcacgcgc gtcgagattg cggtcacgga gaagccgaag
     2881 gggatgaacc gtttcggcgg tttcatgttc cgcctcgcgg cgcgcaagcg catcgaccgg
     2941 gcgctcgagg ggctcgacga gatcgcgggg gaccgcacct gatgcgctgg tggggctggg
     3001 gcgaggatcg cgacgcgatc catctgccgc ctcacgccgt cgacatgctg cgagagcagc
     3061 tcggcatcga tggctcgcgc cgccggcatg ttccgctcga ggaggtgtcg ctaccggagc
     3121 cggctgaggt tcgcctgccc ggtgaggtgc gcaccgaccg cgaggcgcgg gtgctccatg
     3181 cggtgggtcg cggctacccc gatctcgtgc ggatccgttc gggtgacggc tcgagcgctc
     3241 cggatgcagt cgcgtacccg gcctcgcacg atgaggtagc cgagctcttg cgcgcctgtg
     3301 tcgagcagaa cgtggcggtg gtgccgttcg gcggcggcac gagcgtggtc ggcggcgtgg
     3361 agccgctgcg cgacggccat tccgccgtag tcacgttcga cctgtctcgc ctcgacaagc
     3421 tcatctccgt cgaccgggtt tcgttgctcg cgacgttcgc tcccggcttg acgggcccgc
     3481 gcgccgaagc gcttcttgcg agccgcggcc tcacgctcgg gcactacccg cagtcgttcg
     3541 agtacgccac ggtcggcggc ttcgtcgcca cgcgctcggc gggccaggcg tcgaccgggt
     3601 acgggcgcat cgacgagctc gtgcgcggcg tgcggctggc ggcgcccgcc ggcgatttgg
     3661 cagtcaagac cgtccccgcc tctgcggcgg gcccgtcgtt gcgcgagctc gtcgtcggct
     3721 cggagggcgc gctcggcgtt attaccgagg cgacgttcat ggtgcggccg ctgccgcgcg
     3781 tgcgccgcta cgagggctgg tcgttccgat ccttcgccga ggggtgcgag gcgtttcgca
     3841 cgctggagca cgaggactcc tcgccggatg tggcgcggct ttcggacgag gaggagacac
     3901 ggctcaacat ggcgctcgcc tccagcggct cgctggccga tcgtgcgggg cgggcgtatc
     3961 tgcgcgcacg tggccacgag ggtggctgca tcgtcatcgt gggctatgag gggagtgaga
     4021 gggatgtcgc cctcaagcgc ggcttcgctt cggcgatcct gaagcgttcc ggcgggctgc
     4081 cgctcggcac ctcgcccggc cgctcgtggc tccgtaatcg ctatgcagcg ccctatctgc
     4141 gcgacgagct gctcgacctc ggcgtgatgg tcgagaccct cgagaccgcg accacgtggt
     4201 cgaacctgct caatctgcac cgcgcggtgc gcgaggcgct ccacgcgcac gcgccgctcg
     4261 tgatgtgcca cgtctcgcac ctgtaccgct ccggcgcctc gctgtatttc acgttccttg
     4321 gtcgtcagga ccccgggcgc gagatcgatc agtggcgggc cgcgaaggcg gccgcgagcg
     4381 acgcgatcgt ggcgaacggc ggcacgatca cgcaccacca cgcgatcggc cgcgaccacc
     4441 tgccgtggat gccggccgag gtgggcgagc cggggctcga gctgctgcgc gcggcgaagg
     4501 agcggctcga tccggcgggg atcatgaacc ccggcaagct gattccgtag ccaccagtta
     4561 gcaccgcggt gcgaggtcag ctctgatcgg ctacctcctg actttggttc cgcatatgag
     4621 aaccaaactc atgacttagc gccggcggcg cctcgcgcta gcgatatgaa ccctggcagg
     4681 ctgatccccc cgctgaccga cgtcgtcgtt tcgggatact gaccgggacc ccgactcgca
     4741 accacgcggc gacctgccca gcgcgccgcg ggaaggagct tcacacatgg ccttgaccca
     4801 gcgggaaacg cagcaggttg agcaggcgaa cgcgagtggc aggacgccag tggtgttcat
     4861 ccacgggctc tggctgctgc cgagcagctg ggacaactgg gtgtcgctct tccaggaggc
     4921 cggatacgcg ccgctaacgc cggattggcc cgacgatccc gagaccgtcg agcaggcgag
     4981 ggcgaacccc gacgtcctcg cgaagaagac gctcgggcag gtcgccgccc acacctccga
     5041 ggtgatcgga aagctggaca agaaggcggc ggtgatgggc cactcgaccg gcggattgct
     5101 ggcccagatg atcgcgggac gcggtttgtc cgctgcgacg gtcgcgatcg accctggccc
     5161 gtttcgcggc gttctgccgc tgccgttctc gactctgaag gtgacgttgc cggtgctcaa
     5221 gaaccccctc aacaagaacc gtgcgatcac cctgacgctg gaacagttca agtacggatg
     5281 ggtaaacgcg ctcagcgacg aggaggggag acggctctac gagacgtacc atgtggccgg
     5341 gccgggcgtg gcgctgatgc agatggcgaa cgccaacctg aacccgcgga ccgaggcgag
     5401 ggtggacacc aagaacccca accgcgggcc gttgctgatc ctcgacggag agaaggacca
     5461 cacggtgccg tgggcgatcg cgaacgcggc gttcaagagg cagcagcaga acccgggcgt
     5521 gaccgagatc gagaagatcc cgaatcgcgg gcactcgctc acgatcgaca gcggctggcg
     5581 cgaggtcgcc gacaaggcgc tcgcgttcgt gaagcgattc gtctgacgta agagcccgct
     5641 agggcgtcgt cgtagtgccg ctcggcgtgg tgctcgagcc gcccagggcg ttcgctgcgc
     5701 ctgtctgggc gttcgccggc acgttggtca gcttgccgcg caggacgatg ttcgacggcg
     5761 acttcgggtt ggtgccgatc ttctcgcgcg acagcacgat cgagccgaac ttcgtgtagt
     5821 tggcgggcag cggccctgtg ccctggaggt tgccgttctg atcggtgagt tgggcgcaca
     5881 cggcgaacgc gtcgctcttc gagttgtaga gccagacctc gtacgcgttc ttctgcccgg
     5941 tcggctgcag ctgcgcggcc tggaccacga ggctgcgctg gttgccgcgt gtgaacaccg
     6001 cggcgacgcc ggctgccttg ctgccgccgg tcgccttgag cgtgccgacc ttcacgagct
     6061 tggtcttggg cgccgcggcg ttggtgttct tcttgcctcc gccgctgtcg aagacaccgg
     6121 ttagccagag aatcaggccg agcacaacca gtccgccgat cacagcggcg atgagcctgc
     6181 ggcgcttgac ggtgtcggcg acgccgcctc gcaacgagcg atctcgccga ggccgcggcg
     6241 ccgtgcgggc ggtcgcccgc tctggagact cgaccgccgc cgtcagacgt ccgccgaggt
     6301 cgcgccgggc gggccggtcg ccgttgcgct ccgcctcggg gatctccggc gcctcgccgt
     6361 ttggatagag gtcttcgagc gagtcgagca gcgacagcgc ccacgcgcga gcgggctccg
     6421 agcgcttcag atgcccgcgc gtggccgtcg actcgggccc ggtctgctgg ccgagcatgt
     6481 aatcggcgat ctggccgcgc cagtcggggt ccacgcggtc cgcggtgcgg ggcgccagct
     6541 cgcccatcgc ctcgcgggcg agatcccgca cgcgtgaggc gggcatgccc agcatgccgg
     6601 acagttcctc gtatgtctga ccccgctgga ggacgagctc gatgatcgcc cgctgctccg
     6661 cggagaggcg gtcaaaagtg gccaatctgc agggaagggt agtctgcgcg gccgtgtccg
     6721 cgcgtaagaa ctctgtcaga gaagccgaag aggcctacgg cattccgtgg gagaggaccc
     6781 tcgacgggac gctcggcttc gagatcgtcg agattggcga cggaacggcc cgcgcgcgct
     6841 tcgacgtcac ggacagggtg cgccagccct tcggcctcgt acacggcggc gcctactcct
     6901 cgctggccga gtcactgtcc tcagcggcca ccttccaggc ggtggcggac ggcggcaaca
     6961 tcgcgatggg cagctcgaac aacaccacgt tcttccgccc ggtcaccgag ggctcgatca
     7021 acgcagaagc gacagcgcgg caccgtggtc gcacccagtg ggtctgggac gtggagttca
     7081 ccgacgatca ggggcgcctg acggcggcgt cccgagtcac gatcgcggtt cggccgcggc
     7141 ccgcgagccc gcctcgaagc ggttcgtgat cggcaatcgc cggtcgcgcc cgaacgcctt
     7201 caccgagatg cggatcccgg gcggcgcctg acggcgcttg tactcggccc ggtccaccat
     7261 ccggatcacg cgctccacct cgtctccagg cagcccgcgg cgctcgagtt cttcggcgtc
     7321 gagctcttcc tcgatgtagc ccttcaggat cccgtcgagc acgtcgtagg gcggcagcga
     7381 gtcggagtcg cgctgctcgt agcgaagctc cgcggagggc gggcgctcga gcaccgtggc
     7441 cggcacgagc tcgcgcccct cgttggcgtt ccgccagcgc acgaggtcgt agaccgtgtg
     7501 cttgaacacg tccttgagga cggcgaagcc gccggccatg tcgccgtaga gcgtcgcgta
     7561 gcccaccgac aactcggact tgttgccagt ggtgagcacg agccagccga acttgttcga
     7621 gagcgccatc acgaggttgc cgcggatgcg cgcctgcagg ttctcctccg tgatgtcggg
     7681 ctcgcggccc gcgaagacat ccgacagcat cgagtcgaac gcctgcatcg gctgctcgat
     7741 cggcagctcg aatacctccg ccccgacgtt ctgcgcgatc gtccgcgcgt ctgcctgggt
     7801 ctcgtccgac gagtacggcg acggcatgat caccgcgttc accctgtccg gcccgagcgc
     7861 gtcagcggcg acgagcgcaa ccagcgccga atcgatcccg cccgagaggc cgaagacgac
     7921 ctccgagaaa ccgttcttct cgacgtagtc gcgcacgccg gtgacgagcg cccggtagat
     7981 ctcctcctcc tgcgagaggg gaggagcgac ggggccgccc acgtcgtgct cggtgtcact
     8041 gccgctcacc tcgagccgcg ccagcacggt cggctccgcg gagccttcct cccacgagcg
     8101 ctgggcggcg gctcgattgc gcgtgtcacg cagacgcccg gtctcgacgg ccgagggatc
     8161 gaacgtgaag gtgcacatcg actcctcgaa ctgcggcgcg cgggcgatca cctcgccgtc
     8221 gccgtcgatt ccgaggctgt agccgtcgaa tacgagctcg tcctggccgc cgaccatgtt
     8281 gcagaacgcg atcgcgacga tgctgtcgcg cgcccgctgg acgagcatct gctcgcgctc
     8341 tgcgcccttg ccggcgtggt aaggggaggc cgagaggttc acgatgaggt gggcgccggc
     8401 gagcgcctcg gccgtaagcg gcgggcccgg ctcccagatg tcctcgcaga tcgtgatgcc
     8461 gatcagcacg ccgttgacct cgatcagcgc ggcgtcgcgg ctcgactgga agtagcggcg
     8521 ctcgtcgaac acgccgtagt tgggcagata gttcttgcgg tacacgccgg cgacgcgacc
     8581 gtcggccagc acggcggcgg cgttgtagac gtccttgtcg agctcgggga acccaacgac
     8641 cgcgacgatg ccctgggcgg actctgcgat cgactcgagc gcggcgctcg cgcgctcgag
     8701 gaagttccgc ttcagcagca ggtcctcggg cgggtagccc gtcagcacga gctcgggcag
     8761 gacgacgagc gccgcaccct cgtcgcgcgc ggcctcgatc ttctcccgaa tccgcgcggc
     8821 attgcccccg atgtctccga ccgtggagtt catctgggcg agcgcgactc tcatggggtc
     8881 gagcctcgtt cccatgaatt cagtatgacg cggcgctcag ccgctcagcg ggcgtcagat
     8941 gcggttctgg tcgcccacga gcatgcgcaa acgcgtgccc tcagtgctcg cctcgagatc
     9001 gaagcgacgc gtgaggcgct cgatcaggcc gaggccgcgg ccgtgtgtgg cgccccgggg
     9061 ggcgcggttc ctgcggcgga agcggccgca atcgccgacc tcgatcaaga agccctcgcc
     9121 gttgcagccg cagcggacct ctatcggctc cgcggcgtcg gagccgcggc catggcgcat
     9181 cgcgtttgtg accgcctcgg tcacaaccag ggcggcgcgg aagcgcgctt cctcgtcgag
     9241 gccgcacgcc gcagcgcggc cacggacgaa ctcccgtgtc cgggccaact ccgcaggatc
     9301 cggacgaatc tgcagcgtct cgtgcgcgtg cttccccgcc atcggtagtg gcgttcccta
     9361 ctcaaatggg tacaaaacct gactactgcg tcagttaacg ggatggcgcg tgcccaccct
     9421 ccgattgtgg ccctttgcag ggctttaggc ggaaacgagc cgcgtcagcg ggtcagtgtc
     9481 cccaaccgac tggcgcctcg tggcggaccg agtcctcgcc gcgctcgtcg gttcggatgc
     9541 ggaccgactg ctcgaccgga tacacgaaca cgcggccatc gccgatctcg ccggtcctcg
     9601 cgccgcccac gatcgcctcc acggcgcggt cggcgacgtc gtcgggcacc accgattcca
     9661 ccttcacttt ggggagcagc gagatgttcg cccgcgtgcc gcggtactgc tcggtgtaac
     9721 ccttctggcg cccgcacccc atcacctcgg agacggtgag cccggacacg ccgagctcgt
     9781 cgagcgcgtc ctgcacctcc tgcagccgct ccggccgaat gatcgcctcg atcttcttca
     9841 ccgcgtccca gcctccctag aggtcatagc gtgcaagccg gccgggagcg gccccacccc
     9901 ggggcggagc ggcgacccgg cggctggatg gtattcggtg ccagggacgg gcatgaactg
     9961 ctcggggtag ccccacatgc cgtgctccac gaggtcgagc ccgtagcgct cgtcctcggg
    10021 cttcacccgc agcccgtagg tcgacttgat cgcccagaac acgaggtagg acaggacgaa
    10081 tacagccgtg aacgcggata cgacgccgag cgcctggacc gcgagctggt ggatgctgcc
    10141 cgtgtagaag aggccgccct gaccgacccc attgagctcc gccagccgcg gggctgtgaa
    10201 gaggccgcag gagagcgtgc cccagacgcc ggccatgccg tgcgccgaca gcgcgccgac
    10261 cgggtcgtcg agcttgcggt cgatcaggat caccgagaac accacgatca cgcccgcgac
    10321 gaagccgacg atcggcgccg cccagaactc cacgtagccg gacggcgcgg tgatcgcgac
    10381 gagggccgca atcgccccgt tagccgtcat gccgatgtcg aaggtgcgcg tgagcgcgta
    10441 gatcgtcccc atggcgccga gcacgcccgc cgccgcggcc aggttggtga ccacggcgac
    10501 gtcggcgaag cggccgccga tcgcgccaag cgtcgacccc gggttgaagc cgaaccagcc
    10561 gaaccagagg atcaggacgg ccagccccac gaacggcatc gagtggcccg ggatcacgtt
    10621 gggcttcccg tcgcggccgt acttgccctg gcgggcgccg agcagcagca gcgccgcgag
    10681 cgcgccggtc gcaccgatca ggtggacggc ggtcgagccg gcgaagtcct gcatgccgaa
    10741 gttcgcctgg agccagccgc cgccgaacac ccagtggctc acgaccgggt agatcagcgc
    10801 cgagaagacc accgcgtaga tcaggtacgc gccgaacttg atgcgctcga gcgtcgtgcc
    10861 ccacacgatc gcgagcgaga cggcgcagaa gacgaactgg aagaagaact tggccggcac
    10921 cgccgcgctc gagaacgcca ttgcggggaa gagatcgccg ttcgtcgtgc cgaggaaagt
    10981 gccgtgcgtg ccggcgagcc aacctgaccc gccgaacgcg agggcgaagc cgaccgccca
    11041 gtagcagagg cccgcgatcg agaggttggc gagcaccttc gggaccaccg tgcccgcgtt
    11101 cttcatgcgc gagaagccca tctcgagcag gaggaagccg gcctgcataa agaagacgag
    11161 cactgccgcg acgacgaccc agagggtctc gatcgccggt acggcgtcct tgatggtcgg
    11221 tgccaagcgc ggtcctttct ctcgcggttc gagcggcagc cggagcatcc agcgcgcgtt
    11281 ggcagcggtc attcgcccgg cgtacaacat ttgccgcccg cgccttgtac ggcgggcgaa
    11341 gaagcgagtc ctagagcgga atcgagcccg tcagctctcg tcgccgacgg ccgcgacggt
    11401 ggccttgcag cagctcgcga ggaccttcgg gtcgacctcg aacacggtgt tcccgtcgcc
    11461 gccggcggcc cataccgtcg gaaactcgag cagcgcctcg tcgatcacga ccggtagccc
    11521 gtgcgagaac ggcggcacgc caccgggcga gaagccggtc gcggcgcgga cctcatccgg
    11581 gctcgccgcg tgcagcaccg cgcaatcgag cacgtcgcag agcgccctct tgtcgatctt
    11641 gtgggcgccg gaggcaacgc acaccatcgg ctcgccgtcc gcgacgaaga cgatcgattt
    11701 ggcgatccgg gcgggctcgg tgcccacggc gcgcgccgcc tccgcgaccg tgcgcgtcgg
    11761 gcgctcgagc ctcttcatcg aaaccatcag gcccaggtcg cgggccgtgc tcactactcg
    11821 ctcgtgcaac ttcgatactc caaaggggca gacgaagata gtgcaacgcc agacgcagag
    11881 cgccaatcgc cactttggac tattcggtgg gctggaaccg ccgcaccggc gccggagccg
    11941 gtcgcctgga cggccgcgcc ggcgccagct cggcggcgcg ctcgagcgcg gcgaaggagc
    12001 cctcgaagtc ggccacgagg tcctcgatgt ccgacatcgc gtcccagtcg gcgttcagtc
    12061 cgatgttcag gcggccgttg tagctcatga tcgcgacccc gagcgcctgg ccctgcgcca
    12121 gcggcaccat cgggaagatg tcgatcagct cgcgcccgag caggtagagc gggaactgcg
    12181 ggcccggaac gttcgtgacc acgaggttga agaagcgctg ccgcgcggtg aggcgcgcgg
    12241 cctggctcat gatcgtgggt ggggcgaaac cggtcagctc ggtgagcacc tgcgctccga
    12301 ccgcctggcc cgagctcttc aggtcggcca tcgcatccga gatctggcgc atggcctcga
    12361 gagggtcctc gacgcccacc ggcagcggcg ccatcatcgc cgccaccttg ttgccgagcg
    12421 cgcctcgctc gctttgagcc cgcacgctca ccggcaccat cgccttgagc acgaggccgt
    12481 cggtgtcgac cccgcgcccg cgcaggtggc ggccaagcgc ctggctcacc acggtgagca
    12541 ccacgtcgtt caccgttccg ccgagggcgt tcttgatcgc cttcacatcg tcgagcgagg
    12601 cgcgcaccca gcggaagcgg cggtgcggcc cgagcttcac gttgtacggg ctgctcggcg
    12661 ccgggttcag gccggcgccg gccatcgccc cgacgccgcc gagcgccgtc ccggcggccc
    12721 gcagcgcggc acgcggcccc cgcagcgtcg cacgcgcggt gcgcaccatc tcggtcggca
    12781 gcgtggcgcg ctcgagcagc gcctcggcta gcaggtcagc gtgcgacgga agtgggcggg
    12841 cgacccacct cggcggcgga ttgggcggct ccgcaggctc gggcgaggtg tcgaacagca
    12901 cgctcgcgat gtccacgccg gagaccccgt cgacgagtgc gtggtgggtc ttcgagagga
    12961 tcgcgaagcc gccgtcggcg aggccctcca ccagccacat ctcccagagc ggcttgtcgc
    13021 ggtcgagtgg ctgcgagaac acgcgtcccg cgagcgtgcg cagctcatcc tcggagccgg
    13081 gcgggggaag cgccgtacgg cgcacgtggt agccgagatt caggtgcggg tcgtcgaccc
    13141 acgtcggtcg gccctgcccg agcgggacga acgcgagccg ctgccggtag cgcggcacga
    13201 ggtggagccg acgctcgatc gcctcgatca gctcctcgta cgctggaggc gggccgtcga
    13261 acaacatcaa gccggcaacg tgcatgtgag cgccgtcgcg ctccatgtgg aggaacgacg
    13321 cgtcgagccc ggtgagccgg tcgcccgcca tcttggctgc ctactctacg cccgcgtctg
    13381 gctcggagtg ccctgcagtc tgcgggtggc gtccggtcgg gaacgtatgt tcgcctgttg
    13441 tgagcacgga cgcacgtttg atcaggcagg cgtaccggtt cgcgctcgcg ccgagcccgc
    13501 ggcaggaggc gttccttgca tcgtgctgtg gcacgtcgcg gttctggttc aaccagggcc
    13561 ttgcactcgt caaggaccgc ctagaccggc gcggcgcagg tgagcacgcg cgcgtgccgt
    13621 ggtcctacaa gtccctctgc tcggagtttg cgcccctcaa ggacgaggtg tgcccctggc
    13681 gctcggaggt ggtcgtcggc tcgatgcagg cggggctcga gcagcttggg cgcgcgctcc
    13741 agaagtactc caagaccaga gccgctgggc gcccggtcgg gtttccgcgg tttcgggcga
    13801 aggggcgctg ccatgagtcc gtcatctttc agcgcccccg tatagccgac agccgacatg
    13861 tgctgcttga tcgccggctg ggtccgatgc gcacgaaaga gcccatgcga aagctgctga
    13921 ggctgctcgc cgacgacgag cgcgcccgtg tgctgcgctc aacggtcaag cgcacgccgc
    13981 gcggctggac gatcagcttc acagtcgagc gttcgcccaa gcgccgacac gcccgacgcc
    14041 cgaccgccgc cgtcggcgta gatctcggtc tcacgcggct ggcaacgctg tcgaccggcg
    14101 aggcagctgc aaactctcgg ccgttgcggg cggttctggt caagctccgg cgcttgcagc
    14161 gtcagctcga ccgccagcgt cgtgccgcga acgcacgaaa ttacctagct gacgggcgag
    14221 tcagagcggg agcaaagacg tggtcgaaat cgatgagaat gggggacacc gagaagcgca
    14281 tcaagcggtt gcacgcacga gttgcgaacc tacgccgcga acaggcccac cagctcacga
    14341 cctatctgac gcgtgagttc ggcgtcatcg gcgtcgagac actcgccgtc agcaacatga
    14401 tccgcaatcg ccgtctggct cgtcatatcg gcgatgtcgg ctggggcatc atcctgaccc
    14461 aactcaagta caagacctcg tggtccgacg ggtcgctcct tattgcggca aaccgtttcc
    14521 atccctcttc gaggacgtgc agcgcctgcg gagcagtgaa agccaagctg agcctgaacg
    14581 agcgcgtctt cacctgcgat ggccccgctt gcgggcacgt tcaggaccgc gacctcaatg
    14641 cggccttgaa cctcgcctgt ctggctgccc gtcacgcgca ggccgagggc ttgcaatgcc
    14701 acgtcgccgc caccggagcg gagacactaa acgcgcgccg agggcaggtc agactcgacc
    14761 cagtcgagca cagccccgcg aagcgcgaag agtcctcgga ctcatcccag cacggcgacg
    14821 cgctggctct cgcagtctaa gcaggcgggc taccgcctac cgagaagcga gagggggctc
    14881 tacgcttccg gcccgtgcgc acggacgaat tgctcaggcc ctggtacgac gcggctgccg
    14941 cgatggtgcc gggtctgcgc acgttcgacg tgcacacgca taccggtcat gccgacccgg
    15001 acgagttcca ccagacgccc gaggagctac tgacggggct gtccgcggtc gacggccgcg
    15061 cggtcgtctt cacgttgcac gagcccgacg gctactccga gccgaacgac cgcgtccgcg
    15121 cggaggcggc ggcgtccggc ggcaagctcg tgccgttctg ccgcgtgaat ccgcatgccg
    15181 atgcgctggc agaggcgcgc cgctccctcg acgcgggcgc acgcggtatc aagctgcatc
    15241 cgcgcgcgga gcgcttcacg ctcgaccatc ccacggtggg cgacctgttc gagctcgccc
    15301 aggaacgttc ggtgccgatc atcgtgcatg cggggcgggg aataccggcg ctcggtcgcc
    15361 acacgctcga gcaggcggag cgccaccccg gcgcgaacgt gatcctcgcg cacgcgggca
    15421 tctgcgacct ggcgtggctg tggcggcacg gcgaggagcg cccgaacctg ctctacgaca
    15481 gtgcctggtg gaccgcgacg gacctgatcg cgctgttctc gctcgtcccg ccggggcgca
    15541 tcctcttcgg cagcgactcg ccctacggca cgccgatgca cgagtcgatt ctcacgctgc
    15601 ggtgcgctcg ccaggccggc ctgtcggacg atcaggtccg ggagatcatg ggcggccagg
    15661 tcgagcgcct gctcgcgggc gagccgctcg ccgacctcgg tccggcaccg atgaagccgg
    15721 ccgaggccga cctcctcctc gaccgcgcct acacctttct gatccaagcg atcatcgcct
    15781 gcttcgggcg tgtgatggac cgagtgggcg aatcgctcgc cctcgtgcgg ctctgctgcg
    15841 aggtgggcga ggacgcacct caggcggagg cctgtcgatc cgtcctgtcg ctggtggacc
    15901 gcttcgagca ctaccaagcg gttcgcgacg acgacccccg cgacttcgcc ggcgtgtcgc
    15961 tgctgatcgt cgcagcggtg gttgcgagga cgccgaatgt cccgctgccg gagacgccag
    16021 agccggtttc ggtgagcgag cgcaccgact gaggtaggac gcggccacgc ggcggttaca
    16081 gccgcgggga tcttcacatc aagtcaagcc cgcgtgaccg gcgtcacgtt cgtatccctc
    16141 agcaggggta ccgtcgcatg cgaacggttg ttcgaaagag cgccgccgag ccgcgggcct
    16201 caagggccgc gcgggcgggg gaaccaacct gagccgcgtc ccgacagggg actgctggac
    16261 gcggcagggg cgaatcggcc ggttatccgg cagtagcgca gcagagcagc gcgccagtgc
    16321 gcgctgcggg gcgcgcgagc ccgtcagcta accccgtagg tggcgggaaa ggaagttcct
    16381 tgaagtcgat tctggctgcg cgcgtccgca gcgcgcgccg agcggcgccc attgccgctg
    16441 cagcgctcgt gctgggcgct gccgggggcg gcgccgcgat tgcagaggcc gcgggcacca
    16501 ccggtgcgag caccgcggcc ccaacgccac acgtccatgc gaagttcacg cgcgagcagc
    16561 tcgccggcca tcccgtgcgc gctcgcggcg tggctcatcc ctccgatcgc acgcacaccg
    16621 tgctcatgca ggcccgccgc ggccacggcc gctggcagac cgtcgaccgc gcccgcacac
    16681 aggctggaca cttccgcgct tcgtggcggc cgtccagcac ggggcgctac caggttcgcg
    16741 cggtgctcgc cggcgccacc ggggcggcgg gcgccaagcc cgcgcagcgc accgtgcgcg
    16801 tgtaccgcgc agctgccgcg tcctggtacg ggccgggctt ctacggccac aggacggcct
    16861 gcggccacac actcacgccc ggcacgctcg gcgtcgcgaa caagtcgctg ccgtgcggca
    16921 cgcgcgtgac cttccgctac gggcgctacc gcgtaaccgt gccggtcatc gaccgcggcc
    16981 cttacgcggg cgcgcgcgtg tgggatttga cgcctgccac gaagtcgcga ctgcacttcc
    17041 cgtcgaccgg ggtggtgtgg tctacgcggt agagcgcgtc tctccgaagc caggcactga
    17101 gggggtcgcc ccggggccgt gctggcgagc gtcgtcgctc agggtgaata gttcccctcg
    17161 agtgacgacg ctcgccggcg tcgtcatttc cttcccatag tgggatgaaa gtgacgacac
    17221 ttcggcaggg cgggcgaatg ggtaccccta agtgctcaac cggcggggcg ccagggctac
    17281 agcgccaggg cgagctggtc gtcgcccacg ctggagccct cgtcgaggcc ggcgacacgc
    17341 actccgagga ggcgcaccgg cctcggcggg tcgaaatcgc gcagcaacgc gagcgcgatc
    17401 cgcgtgaccg cggccgagtc gttcgtcggc tccgcgaggc tgcgtgctcg cgtgtggatc
    17461 gagaagtcgt cgaggcgcac cttgatgcca atcgtgcgtc ccgactgacc ggcccgtaca
    17521 agccccgcac acagctcgtc cgcgagcttc acgaccacgg gctccatctc gtcgaggcgg
    17581 cgcaggtcgc ggtcgaacgt ggtctcgcgc gactccgact tcgcgacccg gtcggtctcg
    17641 agcaaacgat cgtcctcgaa ccgcgccaga ttcgacaggt gtgggccgag ccgcggcccg
    17701 aacagctcca ctagctgctc agtgggcgcg gcggcgagat cccccagggt cctgatgtcg
    17761 ttctcttcga gccgcttcac ggtcttcggc ccgatgccgg gcacgagccc gggcgaggcg
    17821 gccgcaaacc gcgcgcacgc ctcctcgcgc gacagaaaca cgaagccgtc gggcttctcg
    17881 gcgtcggagg ccactttggc gacgagcttg ctcggaccca ctccgatcga gcacacaagg
    17941 cccgtcgcct cccgcaccgc ctccttgatg cggcgcgccg ccgcgcgcgg gcgatcgatc
    18001 ccggtgaggt ctaggtaggc ctcgtcgagg cccacgatct cgaccgtctc gacctgcgcg
    18061 cgcagcacct ccatcacctc gcgcgaccgc gcgcggtagt gctcgaagtc cggcgggatg
    18121 aacaccgcgt ccggacagcg gcggcgcgcc cactcagcgg gagtggccga ctgcacgccg
    18181 aatttgcgcg cctcgtacga ggcggtcgtg accaccgcgc gcgggctcga gcccgccacg
    18241 acgaccggct tgccgatcag ctcggggcgt cgctgaagct cgacgctcac gtagaacgcg
    18301 tccatgtcga catgcgcgat gcagcgctcc atcgcgagca ggctaggggc gcgggcggac
    18361 aggcgttcgt gcccggctag gccggcgcgc ccgcgccgct ttcctcggta acgagcgcct
    18421 cgagcgatgc cttcacgccg cgcggcagca cgatcacggg gcagtgcgct tccgagacga
    18481 cgtgcctcga cacgctgccg agcagcaccg cgcgcgcagg gccatagcca cgtgagccgc
    18541 acacgaggag atcgacgccg ttcgatagct cgacgagcgc ctcgccgggg tcgccgatca
    18601 cggcctcgac ctcgacagac acgtcgtcgc cgagctcgcc cacggctacg cgcagccgcc
    18661 gctcagcttc gagccggtgc tctccctcca cctcctcgag cgacttcccg gcctggctgg
    18721 cggtcgcagg ctcggtctcg aagtgcatgc tcgcggtcac cttcacgacc gtgatcacgc
    18781 gcaacgtcgc gccggcacgg cgtgcgagcg cgtacgcgcc gcgcagcgct tcccgggcct
    18841 cttcggtgtc tacgtaggcg acgccgatga cggatggagg tcctccacga gtccatgccc
    18901 gcggcacgac cgtgaccggg cacggcgcac cgtgcaggag acggacggcc gtgcctccgg
    18961 cgagcgcacg cccaacgccc gacctccgcg atgagccgat cacgaggagg cccgcgtcat
    19021 cgtcgtcggc ttcccggtgg agcgccttcg cggcgctcga gctctggagc agcctgacct
    19081 cgtatgcgat cccctcgtgc tcgaggtgct gctggacggc gcgcgccgct tccgacgcgt
    19141 cgtgcgtcag atcagcatcg gtctggccga cggcatagtc gagaggctcg ccggagacgg
    19201 gtagcgacgg caccttttcc gacgtgacgc acgcgactat caaccgtgcg cccgtgaagc
    19261 gcgccgcggc gatgccgaat gacaatgggg cgttgtcgat agtcctcggg tcgtatccga
    19321 cgaggatggg cttccccatg tccccatggt ctgtgcgccg cgcgcgggtg tcaactaaga
    19381 aggctggcgg acaggcctga tcagtatctc gttcacgtcc acgtgcgccg gctgcgacag
    19441 ggcgaacacg acggcgcgcg cgatgtcgtc ggcttcgagg gcgacctcgg ggcggttgtc
    19501 gaagaagggc gtgtccaccg cgcccggctc gatcagcgtc acccggatgt ccgagtcgga
    19561 cacctcctgg cggagccctt cgcccatcgc cgtcacggcc cacttcgtgg ccgagtagag
    19621 cgaccctggc agtgcccggc ggccggcgac cgagctcgtg agcacgacgt gaccgcggcc
    19681 ctcgttgcgg aagtgcgcga gcgacgcccg tatcgagagc gcggtgccgt agacgttggt
    19741 gtcgatcatc gagcgccaga actccggcgt ctcttcgagg aagccgcgct tcgccccgaa
    19801 gccggcgttc gcgaacattg cgtccaggcg cccgaagtga tcgagcgcgg ccgccgccag
    19861 cgcctgctgg tcgttccagt cggtcacatc gcaccgacgt ccgatcgcca cctcgtcgcc
    19921 tccgagctcg gccgccagcg cggcgagccg gtcctccgag cgcgcgccga gcacgactcg
    19981 ccacccggcc tccgcggcat gccgcgcggt cgcggcgccg atgccgctcg aggcgccggt
    20041 gatgacgatc acgggtcgct catccgccac gcgcgggttg tatcagggcg agtacgctgc
    20101 gcgccgtgaa cgcgggagag ttgcgcacgc ggccgcgttt tccggacgtg ccgcgcgagg
    20161 cagggcacta cgagagcttc tacctgaagg catcgcaccc gaccga
//
LOCUS       DASVXS010000196        15483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1087587, whole genome shotgun sequence.
ACCESSION   DASVXS010000196 DASVXS010000000
VERSION     DASVXS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15483
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087587"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..789)
                     /locus_tag="VF752_10825"
     CDS             complement(151..789)
                     /locus_tag="VF752_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856084.1"
                     /translation="MAEYAVAHLEEIDELSDGRAPFRPIRHELGITSFGVNTWTGHEA
                     GDRIINEHDEAGWDEELYFVQSGRATFELDGERVDAPVGTFVFARPGVKRTAFAEEPG
                     TTILAVGATPGQAYEPEGFEIWAPFNRLYLEGRYAEAADGGREAVKAHPEYAGPLYNL
                     ACCESLAGRTADAIEHLRLAIDKAERYRSFAIGDSDFDPIREEPAFQALVTA"
     gene            870..1265
                     /locus_tag="VF752_10830"
     CDS             870..1265
                     /locus_tag="VF752_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856085.1"
                     /translation="MSQENVEVVRRVYDAWNAGDMTALRDLCDPDVVMHHPEGWPEPG
                     PSVGREAVMRQWEQIREAWTGDTLVPVTDFVAAADRVVVRDSWRGTGQGPDADMQFSR
                     VFTLRERKIIGVDIFWDHADALETVGLRE"
     gene            complement(1290..1715)
                     /locus_tag="VF752_10835"
     CDS             complement(1290..1715)
                     /locus_tag="VF752_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856086.1"
                     /translation="MSQENVELVRRLYAELASEGSTQQFEQRMSDDALGRFLDPEIEW
                     VPVTESLLAVDSYRGFDGVRRFWGEFLSAWERYRVETLRFDDAGDQVAVVVHIVGRTH
                     ELEVDETRSSLLTVRDGRVVRVQSFADPDGARQAAGLPP"
     gene            1768..2553
                     /locus_tag="VF752_10840"
     CDS             1768..2553
                     /locus_tag="VF752_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8856087.1"
                     /translation="MPSPDRLDFYDGELRAHHEHLRAAYGISPGDDVVDIGCGTGLTT
                     REAARASAPGRVVGVDVSERMLERARQLTAAERIDNVRYELGDAQVHRFDAAGFDVAI
                     SRFGTMFFSDPAVAFANIAAALRPEARLVLLVWQRHEHNEWARAIDAALGDAAQPRQP
                     GADPFSLGDVKTTAGILEGAGFDGVRFEDVHEPVLYGHDLDAAIAFVRGFQDTSAAFA
                     TLNDGEAARTVERLREMLAAHHSDERGVVLDSRSWLITARRRR"
     gene            complement(2604..3458)
                     /locus_tag="VF752_10845"
     CDS             complement(2604..3458)
                     /locus_tag="VF752_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8856088.1"
                     /translation="MRLVTYDSGRGPRAGVLSGEHGIVDIWDSLGEEPRCEPSLSEVI
                     RAGLLEKARGVEGAEPTAHVHESALLPPILDPGKIICLGLNYGSHAEETGREAPDQPT
                     IFGKYANALAPPGATVKLPAYSEKVDYEAEVAFVVGERSKDVSEADAIAKLAGYMLLN
                     DLSARDFQFKTPQWMPGKIFDGAAPCGPALVTPDEAGPPDRIEIQLTLNGEVMQHATT
                     ADLIHSIPALVVHLSMLMTLEPGDIVSTGTPAGVGSVRTPRVWLKPGDEMVVSSPQLG
                     TLETTLAR"
     gene            3494..4474
                     /locus_tag="VF752_10850"
     CDS             3494..4474
                     /locus_tag="VF752_10850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8856089.1"
                     /translation="MRRPVLLAALAATMFFVLPAGADAPKRLPSGATIAGVSVAGMGP
                     VTAQREVQRVIGAQYERPITLRVGSRDVAISTAKAGQTIAYKRMVDLAFKLAGDHKPV
                     HVRLDRDYDSNALTAAVKAASKPFYRAPRNARVRFGVTRVVRIRGHNGRGLDLKSVRV
                     AVGAELHRPTDARIITASVRTLRPRIGLNKLRSVYSTYISVDRGHFKLRLFKRLRVVK
                     TYPIAVGMAGHDTPRGLRHVLSKEKNPAWHVPNSPWAGSLAGTTVPPGSPNNPLKYAF
                     IALGDGVGIHGTAEDWSIGSRASHGCIRMHVSDVKRLYPRVPVGTPVLIH"
     gene            4525..5106
                     /locus_tag="VF752_10855"
     CDS             4525..5106
                     /locus_tag="VF752_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX8856090.1"
                     /translation="MRKLIDEFGPLDEEERRRGRPAEAYGALLRSIVGQQLSTKAARS
                     IYGRLVELFGGHTPTPEELLAADPEEVRSVGMSRPKVAYLRSLAEHVIDGELELDRLD
                     ELSDEEISAELTAVKGLGQWTADMFLMFHLRRPDVLPVGDQGIRRAVERAYGLKKIPD
                     AAKLEKIAEPWRPHRTLACLYLWASLDNKPVAE"
     gene            complement(5316..5672)
                     /locus_tag="VF752_10860"
     CDS             complement(5316..5672)
                     /locus_tag="VF752_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX8856091.1"
                     /translation="MVSLTDVAAEKVSQFLSGQSAGETAGLRVGVRGGGCSGFQYALA
                     LDEQREGDHIFEDRGIRVIVDEASLQYVNGSTVDYKESLMGAGFEVNNPNVVAACGCG
                     SSFRVADEDPSCGATV"
     gene            5752..5988
                     /locus_tag="VF752_10865"
     CDS             5752..5988
                     /locus_tag="VF752_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX8856092.1"
                     /translation="MPNAEELKERIQNALPGAQVAVEDLTGGGDHFRAEVVSERFDGL
                     SRIEQHKLVYDIFGDEIGGAIHALSMKTSTPGGN"
     gene            5988..6389
                     /gene="grxD"
                     /locus_tag="VF752_10870"
     CDS             5988..6389
                     /gene="grxD"
                     /locus_tag="VF752_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX8856093.1"
                     /translation="MENQEIREFLENAINENRVMLFMKGTPDAPRCGFSLRTAAAVNA
                     MGVQYAAMDILPDPRIREELSAISGWPTIPQLFIDGKLVGGCDIVLEMYESGELAALL
                     GVEQPEDDEELSPQSPGQQDSQPLGLENRLN"
     gene            complement(6386..6598)
                     /locus_tag="VF752_10875"
     CDS             complement(6386..6598)
                     /locus_tag="VF752_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856094.1"
                     /translation="MAGYRRWAPMARRGFLIALEAKRRWDRLPEHEKQRYIKMARDYA
                     QRGRALGGVAMKRGRDLYGSRPKKKR"
     gene            complement(6598..6882)
                     /locus_tag="VF752_10880"
     CDS             complement(6598..6882)
                     /locus_tag="VF752_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8856095.1"
                     /translation="MATLYRCRTRTNLLCACGRVARRLNRAGIEYDEVRVPYLKRYRP
                     EVEELTGQRWVPVLVHGEQVIHDSRRICEYIDHLESTSPPSRDAALGESG"
     gene            complement(6906..7985)
                     /locus_tag="VF752_10885"
     CDS             complement(6906..7985)
                     /locus_tag="VF752_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8856096.1"
                     /translation="MGKTLVTGGTGFIGSHIARLLAERGDDLRLTLREDSRLEGLEGL
                     DYEGVRCDLLDRRAVRRALKGVDRVFHSAGLLSLRPADADVLFQVNVGATRLLFEECL
                     RAGVVRVVYTSSVAAIGPAARGGTADENALFTAGHLQIPYVNSKHEAEVEAMRHAAKG
                     LPVVCVNPCFVFGSGDVTGGSTGVVRRFLLGRIPVYIEGGLNVVGVGDVARGHLLADE
                     RGAVGERYILGNRNYTWDRFFADLARMSGCEPPPLKLSPGLALRLAQALDAAPGRPPI
                     SVDEVKAAGQWWTYRSTKAQRDLGWKPSPHEEAVEATVAWYMERDGARVARSRRSQPI
                     QYKAAARALGAVEGAAAIARRLSPV"
     gene            complement(8025..8294)
                     /locus_tag="VF752_10890"
     CDS             complement(8025..8294)
                     /locus_tag="VF752_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX8856097.1"
                     /translation="MLTGSDRDRVEALLRERADGMVRGRRERRSQDADEHAVEGGEHA
                     RIEAHRARRTRVCVACGTTIAPARLELVPEAVRCVPCQQAHEAAP"
     gene            8360..9223
                     /locus_tag="VF752_10895"
     CDS             8360..9223
                     /locus_tag="VF752_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX8856098.1"
                     /translation="MSPVAAVTDTTGYLPADVVRDNSIHIVSLYVNFGGERTEREADI
                     TDYGRFFEELRRSEQLPTTSQPAVGDFIGVYEPLLTQDQDIVSVHISGGLSGTVDAAR
                     QAAQALEGKGGERVHLFDSETGAGGTGILALVAARAAQRGESAEQIMERLRGARADLK
                     IWFAIDTLEYLRRGGRIGAASAWIGSTLKIKPILTVEREMTPVERVRTSKRAFERMVD
                     YAKQRHSSGADAWVVQHIQSPDQAAELTARCTEIFGTGPLFVSEIGPVLGVHTGPGLL
                     GVGGIPPHWLD"
     gene            complement(9220..9441)
                     /locus_tag="VF752_10900"
     CDS             complement(9220..9441)
                     /locus_tag="VF752_10900"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX8856099.1"
                     /translation="MPTRSPDEIRRSVQATRTELTYSLNDLQGKVQQLTDWRQQLRQN
                     KQTAMIVAGVAGFVIGGGIAATFGLFRRR"
     gene            complement(9441..9863)
                     /locus_tag="VF752_10905"
     CDS             complement(9441..9863)
                     /locus_tag="VF752_10905"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8856100.1"
                     /translation="MTDHNGAPPGSAEKSLGEIVNEVSEKASLLVREEIQLAQAEIQV
                     KINRLAKGAAFGAAAGVFLVFVLIYFFHALSWLWDDLFDFNSVWPGFAITAGLLLILG
                     AIAGLLAVRFFKRGAPPVPELAIEEAKKTRATLEEARH"
     gene            complement(9856..10116)
                     /locus_tag="VF752_10910"
     CDS             complement(9856..10116)
                     /locus_tag="VF752_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856101.1"
                     /translation="MTQEDTATHTSASEQPTAETDALGREAAETAAGGSYSAVATGQS
                     PLDAHTRAVEQSSDRPEILVGAAFAGGFLVAQVLKRLGGGDD"
     gene            complement(10121..10255)
                     /locus_tag="VF752_10915"
     CDS             complement(10121..10255)
                     /locus_tag="VF752_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856102.1"
                     /translation="MSNDMVKRLAWSGLLAGIGALSTIVANRIATIIWQRVFNEDPPE
                     "
     gene            complement(10285..10917)
                     /locus_tag="VF752_10920"
     CDS             complement(10285..10917)
                     /locus_tag="VF752_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8856103.1"
                     /translation="MASTRQHPNGVDKRRLILDAAVRVFAQQGFHACRVSDVADEAGV
                     AYGLVYHYFDSKEEILNTLFTERWQIMLDAIVDIDRQEVPARDKLYMVASFIIDSYRY
                     DPNLMKVIIVEVTRAANSFGRQHLEKIREAYQLIAGIVESARVDGSFKSDISSDFAAM
                     CFYGAIEQLLSGWIFDLMPQQDEDFERAKGLVVETICGGLEAPSASAPVS"
     gene            complement(10917..11744)
                     /locus_tag="VF752_10925"
     CDS             complement(10917..11744)
                     /locus_tag="VF752_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8856104.1"
                     /translation="MRAAAVQLNSTEDKERNLGTADRLTRAAAANGATLVVLPEKFNV
                     LGTHDDYIAGAETLEGPTISWARDTARELGIDLVAGSIVERREGHDKLSNTSVHIGPD
                     GELKGVYRKIHMFDVVVGGVEYRESESEEPGDEMTITEAQGVPVGMTVCYDLRFPELY
                     RILAVRGARVLTLPAAFTKVTGRAHWEILVRARAIENQAFVVAADQIGATSDGKESFG
                     GSMIVDPWGEVLARAPDEEMFIAADLNLERQDEVRRELPSLANRVPSAYRWPQEAIA"
     gene            11876..12706
                     /locus_tag="VF752_10930"
     CDS             11876..12706
                     /locus_tag="VF752_10930"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HEX8856105.1"
                     /translation="MASAGNLPARGARVRDLGLALPPGRMPLLQHGRPLKRWRYVGVF
                     GPELMLCAGDARVGPFPQRWWAVALPDGTLRERTTAGRGGVIIEPRRLRVAAPNARIN
                     VNLDESGTELEVVSPSGKSGYIWTRKRGGVPARGRAVIDGRVLEVDAGAVIDESAGYH
                     ERHTSWRWSAGVGHTDTGEPVAWNLVDGVHDAPEQSERTLWIGDEARELPPVEFAADL
                     SRVGGLSFRAWATREHHTNLWLFRSDYRQPFGTFEGELAPGVRLAEGWGVMEEHDAWW
                     "
     gene            12697..14412
                     /locus_tag="VF752_10935"
     CDS             12697..14412
                     /locus_tag="VF752_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856106.1"
                     /translation="MVVTVERRAAPPASRRGARESPALPGRELALAALAAVLIACVMH
                     WPIPINLGSDVPRDLGDPLTQAWQVAWGGHALLHQPLDYFQANDFWPLKDPLAFSDAL
                     VGYAPAGMIGSGPHAAIVRYDLLFLFAYALAFLGAYLLARELGARPIPATVAGAAFAY
                     APWRLEQDGHLHVISSGGIPLALFLLLRGYRRANPVTVAAGWVVVTWQISLGFTLGLQ
                     LLYLICLLGAGAAVLIWRKRLPLPPRRVLAATAGGVALTAAVSAALAVPYARVLDRHP
                     EAKRTLAQVSSLSPPLKAFAAAPPQNLIWGKATEGVRNDVRWVPEMSLFPGVAILALA
                     LTGLFWRGAPRGLRIGLGVSAVVVWLLSLGIHGGGPWPYRLLYEVLPGWQGIRVPGRL
                     MTLTSLALALLAAFGAQRILAARRVPAAVGVGLVVLVLLEGSGLGIDERGAAIAGPAH
                     PTVPPQPSGQIGAPAPQAHLPLDTLDSRKYLLWSTDRFPELVNGRGSFRPAEFKRLQV
                     ELASFPDARSVAVLQQLRVRTVILHPTFVRGTPWAAWRARPVSGLPLRREERGGVVLY
                     QLLPR"
     gene            complement(14345..>15483)
                     /locus_tag="VF752_10940"
     CDS             complement(14345..>15483)
                     /locus_tag="VF752_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856107.1"
                     /translation="SHVLSACLGFAAFVVLWVEREGPQHLGRVALAGALAGLAITCEY
                     PLGLVAVIVGLYALARRDRLRRAFAYGGGAVVGLLPLFGYNLWAFGQPLHSGYADAVA
                     IQGASGHDVLGLNSSGFFGIGAPSLKVAADLLFSAKGLLITSPLLALGIVGTVLLYRS
                     GKRAEALVVAAVGLSYLVYNAGYYLPFGGGSPGPRFLIPAIPFLCAPLALAFKRWPAA
                     VLGLAVASTVTLATATITLPLIGNQDVGYWVKLVGAHSFQNTFLTVLGLGRGWLGITP
                     FLVALVAAGVLAAWATRPMRIVRRDVLTAALALAAWAGVAATVPDFLGRGDGDVAHTS
                     QALALVAVSMGAGLLAVLLAAAFSAEAAGRAPRRRAPRGEAATR"
BASE COUNT         2331 a         5403 c         5348 g         2401 t
ORIGIN      
        1 ccgcatttcg atacggacta ttcgacaaga cgctcgggcg ctggttcatc aagcgctggg
       61 ccgaacaggc catcgaaggg ttacgccgga cgctgacttc acgcgctaag gcctagtgcg
      121 cgagcccccg ctcttgcggc gggccgggag tcaggccgtg acgagggcct ggaacgcggg
      181 ctcctcgcga atcgggtcga agtcggaatc gcctatggca aacgagcggt aacgctccgc
      241 cttgtcgatc gcgagcctca ggtgctcgat cgcgtcggcc gttcggccgg cgagactctc
      301 gcagcaagcc aggttgtaga gcggtcccgc atactcggga tgcgccttca ccgcttcacg
      361 ccccccgtcg gccgcctcgg cgtagcggcc ttcgaggtag aggcggttga acggggccca
      421 gatctcaaag ccttctggct catacgcctg acccggggtg gccccgaccg cgaggatcgt
      481 cgtcccgggc tcctctgcga acgcggtgcg cttcaccccg gggcgggcga agacgaaagt
      541 gcccaccggc gcgtccacgc gctcgccgtc gagctcgaag gtcgctctgc cgctctggac
      601 gaaatagagc tcttcgtccc acccggcctc gtcgtgctcg ttgattatcc ggtcgccggc
      661 ctcgtggccg gtccatgtgt tgacgccaaa cgacgtgatg ccgagctcgt gacggatcgg
      721 gcggaaggga gcgcgaccgt cgctcagctc gtcgatctcc tcaaggtggg caacggcgta
      781 ttcggccatt gcacgtagtg tcgcgcttct gaatgggacg gagcggcggt ggcggaagcc
      841 gacgctggta gcgggatact gcaggggcga tgtcgcagga gaacgtcgag gtggtacgcc
      901 gtgtctatga cgcgtggaac gccggagata tgaccgcgct ccgcgacctc tgcgaccccg
      961 atgtggtcat gcatcacccc gagggctggc ctgagcccgg cccctcggtg ggtcgggagg
     1021 cggtcatgcg ccagtgggag cagattcgcg aggcttggac gggcgacacg ttggtgccgg
     1081 tcaccgactt cgtcgccgcc gccgaccgcg tcgtcgtccg ggattcctgg cgtgggacgg
     1141 ggcaaggacc cgacgcggac atgcagttct ctcgcgtctt cactctgcgg gagcgcaaga
     1201 tcatcggcgt cgacatcttc tgggaccacg cagatgccct cgaaaccgtg gggcttcggg
     1261 agtagcggct cccgcttcgg atgatccgcc tatggcggca acccggcggc ctggcgcgcc
     1321 ccgtcggggt ccgcaaagga ttggacgcgg acgacacgcc catcgcggac ggtcaagagc
     1381 gaggaccggg tctcatcgac ttcgagctcg tgcgtccggc cgacgatgtg tacgacgacc
     1441 gccacctgat caccggcgtc gtcgaacctc agcgtctcaa cccggtacct ctcccaagcc
     1501 gacaggaact caccccagaa tcgccgcacg ccgtcaaagc ctcggtaact gtcgacggca
     1561 agcaatgact cagtcaccgg gacccactcg atctctggat ctaagaaacg accgagagcg
     1621 tcatcggaca tgcgctgctc gaactgttgt gtggagccct cgctcgcaag ctccgcgtag
     1681 agtcggcgga ccagttcgac gttctcctgt gacacggcgt tccccagtgt ctcgcagcgc
     1741 gctccctggt tggttagcat cgcgtcggtg ccctcgccgg acaggctgga cttctacgac
     1801 ggcgagttgc gcgctcacca cgagcacctt cgcgcggcct acgggatcag ccccggtgac
     1861 gacgtggtcg acatcgggtg cggaaccggg ctgacgactc gcgaggcggc gcgcgcctcg
     1921 gcgccgggcc gcgttgtcgg cgtcgacgta tccgagcgca tgcttgagcg cgcccgccag
     1981 ttgacggcgg ccgaacggat cgacaacgtc cggtacgaac taggcgacgc ccaggtgcat
     2041 cgcttcgacg ccgccggctt cgacgtggcg atcagccgct tcgggacgat gtttttctcc
     2101 gatccggccg tcgcgttcgc caacatcgcc gccgcccttc gacccgaggc gcgcctggtg
     2161 ctgctcgtct ggcagcgaca cgagcacaac gaatgggcgc gggcgatcga cgccgcgctg
     2221 ggtgatgcgg cgcagccacg gcagccgggc gcggaccctt tctcgctcgg cgatgtcaaa
     2281 accaccgcgg ggatcctcga gggcgccggc ttcgacggcg tgcggttcga ggacgtccac
     2341 gagccggtcc tctacggaca cgacctcgac gccgcgatcg cgttcgtccg gggatttcag
     2401 gacacaagcg cagccttcgc caccctgaac gacggcgagg cggcccgtac cgtcgagcgc
     2461 ctccgcgaga tgctcgcggc ccaccacagc gacgagcgcg gcgtcgtcct cgattcgcgt
     2521 tcctggctga tcaccgcccg gcgacgccgc taaggcgttg agaaaaccgg atcccccggc
     2581 gcggactgcc agaggggcgg cgcctagcgc gcgagcgtcg tctcgagcgt gccgagctgg
     2641 ggcgagctga cgaccatctc gtcgcccggc ttcagccaca cgcgcggcgt gcgcacactg
     2701 cccacgccgg cgggcgtgcc ggtggacaca atgtcgccgg gctcgagcgt catcagcatc
     2761 gacaggtgca ccactagcgc ggggatcgag tggatcaggt cggccgtggt ggcgtgctgc
     2821 atcacctcgc cgttgagggt gagctggatc tcgatccgat ccggcggccc ggcttcgtcg
     2881 ggcgtgacga gcgcgggccc gcagggtgcg gcgccgtcga agatcttgcc gggcatccac
     2941 tgcggcgtct tgaactggaa gtcgcgcgcc gagaggtcat tcagcagcat gtagccggcg
     3001 agcttcgcga tcgcgtcggc ctcgctcacg tccttggagc gctcgccgac gacgaacgcc
     3061 acctcggctt cgtaatccac cttctcgctg taggcgggca gcttcaccgt cgcgccgggc
     3121 ggcgcgagtg cgttcgcgta cttgccgaag atcgtcggct ggtcgggcgc ctcgcggccg
     3181 gtctcctccg cgtgggagcc gtagttcagc ccgaggcaga tgatctttcc cgggtcgagg
     3241 atgggcggca gcagtgcgga ttcgtgcacg tgtgccgtcg gctccgcgcc ctcgaccccg
     3301 cgcgccttct cgagcaggcc ggcgcggatg acttcgctga gcgacggctc gcagcgcggc
     3361 tcctccccga gcgagtccca gatgtcgacg atgccatgtt cgccgctgag cacgccggcg
     3421 cgcggacccc gccccgagtc gtaggtgacc agcctcatcg cggcggcaaa ttagcgcagt
     3481 taccctgttg ggcatgaggc gaccggtact actcgccgcg ctcgcggcga cgatgttctt
     3541 cgtgctgcct gcgggggcgg acgcgccgaa gcggctgccg tcgggggcga cgattgcggg
     3601 cgtgtccgtc gccggcatgg gccccgtgac cgcgcagcgc gaagtgcagc gcgtgatcgg
     3661 cgcgcagtat gagcggccga tcacgctgcg ggtcggctca cgcgacgtgg cgatctcgac
     3721 cgccaaggcg ggccagacga tcgcctacaa gcgcatggtt gacctggcgt tcaagctggc
     3781 gggcgaccac aagccggtgc acgtacgtct cgaccgcgac tacgactcga acgcgctcac
     3841 ggcggcggtg aaggcggcct cgaagccgtt ctaccgcgcg ccacgcaatg cgcgcgtgcg
     3901 cttcggggtg acgcgggtcg tgcggatccg cggccacaac ggccgcgggc tggatctgaa
     3961 atcggtgcgc gtcgccgtcg gggcggagct gcaccgtccg accgatgcgc gaatcatcac
     4021 cgcctccgtg cgcacgctgc gcccgcggat cgggctgaac aagctgcgga gtgtctactc
     4081 gacctacatc tcggtggacc gcggccactt caagctgcgc ctcttcaagc ggctgcgcgt
     4141 ggtgaagacc tatccgatcg cggtcggcat ggcggggcac gacacaccgc gcggtcttcg
     4201 tcacgtgctc tccaaggaga agaacccggc gtggcacgtg ccgaacagcc cgtgggccgg
     4261 ctcgctcgcg gggaccacgg tgcctccggg gtccccgaac aacccgctga agtacgcgtt
     4321 catcgcactc ggggacggcg tgggcattca tggcaccgcc gaggactggt cgataggctc
     4381 gcgcgcgtcg cacgggtgca tccgcatgca cgtgagcgac gtcaagcgcc tctatccgcg
     4441 cgtccccgtc gggacgccag tcctgatcca ctgacccccc gctccaactc ccccgtcgag
     4501 gacctccgca aggccgatcc ggtcatgcgg aagctgatcg acgagttcgg gcccctcgac
     4561 gaggaggagc gccggcgcgg gcgcccggcc gaggcgtatg gcgcgctgct gcggtcgatc
     4621 gtggggcagc agctgtcgac gaaggcggcg cgctcgatct acgggcggct ggtggagctc
     4681 ttcggcgggc acacgccgac gccggaggag ctgctcgcgg cggatcccga ggaggtgcgc
     4741 tcggtgggga tgtcgcggcc caaggtcgcg tacctgcgct cgctggccga gcacgtgatc
     4801 gacggggagc tcgagctcga ccggttggac gagctctccg acgaggagat ctctgccgag
     4861 ctgacggcgg tgaagggcct cggccagtgg acggccgaca tgttcctgat gttccacctg
     4921 cggcgcccgg acgtgttgcc cgtgggcgac caggggatcc ggcgcgcggt ggagcgagcc
     4981 tacgggctga agaagatccc ggacgccgca aagctcgaga agatcgccga gccgtggcgg
     5041 ccgcaccgga cgctggcctg cctgtacctc tgggcgtcgc ttgacaacaa gccggttgct
     5101 gagtagcggg gcgccgtgaa cagcagcaat aaggagtggc ccgttggggt ggggtacgcc
     5161 atgcgtggta ggcggccggg acgtgggtga ctttggggtc gaatagcgac cctaaggtca
     5221 cccacgccta gagcgggcta gcccacgcga ggtaccgccg cggaccgggc ccacccgtta
     5281 ttgctgctgt ttcggagaac cgcgcggagc gcgcgctaga ccgtcgcgcc gcagctcgga
     5341 tcctcgtccg ccacgcggaa cgaggagccg cacccacagg ccgccacgac gttcgggttg
     5401 ttcacctcga agcccgcgcc catgagggat tccttgtaat cgaccgtcga gccgttcacg
     5461 tactgcaggc tcgcctcgtc gacgatcacg cggatgccac ggtcctcgaa gatgtggtcg
     5521 ccctcgcgct gctcatcgag cgctagcgcg tactggaagc cggagcagcc gccgccgcgc
     5581 actcccacgc ggagaccggc ggtctcgccg gcgctctgtc cggagaggaa ctgggagacc
     5641 ttctctgctg ctacgtctgt gaggctgacc atctgggatc gattgtagcc gcaactgccg
     5701 tcggccattc ctgtacgaga gatcgctctc gctatctttg atcggacgcc gatgccgaac
     5761 gctgaagagc tgaaggaacg gatccagaac gcgctgcccg gggcgcaggt cgcggtcgaa
     5821 gacctaacgg gcggcggcga tcacttccgc gcggaagtcg tttccgagcg cttcgacggc
     5881 ctctcacgca tcgagcagca caagctcgtc tacgacatct tcggcgacga gatcggcggc
     5941 gcgatccacg cgctctcgat gaagacttca acgccagggg gcaactgatg gagaaccagg
     6001 agattcgcga gttcctcgag aacgcgatca acgagaaccg cgtgatgctg ttcatgaagg
     6061 gcacgcccga cgcgccccgc tgcggcttct ccctgcgcac ggcggccgct gtgaacgcca
     6121 tgggcgtgca gtacgccgcg atggacatcc tgcccgaccc gcgcatccgt gaggagcttt
     6181 ccgcgatttc gggctggccg accatcccgc agctgttcat cgacgggaag ctcgtcggcg
     6241 gctgcgacat cgtgctcgag atgtacgagt ctggcgagtt ggccgcgttg ctcggcgttg
     6301 agcagcccga ggacgacgag gagctgagcc cgcagtcgcc gggacagcag gattcgcaac
     6361 ccctcgggct cgagaaccgc ctgaactagc gcttcttctt gggacggctg ccgtagaggt
     6421 cgcgcccgcg cttcatcgcg acaccaccca gggcgcgccc gcgctgggcg tagtcgcgcg
     6481 ccatcttgat gtagcgctgc ttctcgtgct cgggcagccg gtcccagcgc cgcttcgcct
     6541 cgagcgcgat caggaagccg cgcctggcca tcggcgccca gcgcctgtat cccgccatca
     6601 accgctctcc ccgagcgccg cgtcgcggga cggcgggctc gtcgactcga gatggtcgat
     6661 gtactcgcag atgcggcgcg agtcgtggat cacctgctcg ccgtggacta gcaccggcac
     6721 ccagcgctgt cccgtcagct cctccacctc gggcctgtat cgcttcagat agggcacgcg
     6781 cacctcgtcg tactcgatcc ccgcccggtt caaacgccgg gcgaccctac cgcaagcgca
     6841 gagcaggttc gtgcgggtcc tacagcggta cagcgtggcc atgccgcgag gttagcggca
     6901 cctcgtcaga cggggctgag ccgccgcgcg atcgcggccg cgccctccac cgcgcctagc
     6961 gccctcgcgg ccgccttgta ctggatcggc tgcgatcggc gggagcgcgc cacgcgggcg
     7021 ccgtcgcgct ccatgtacca ggcgaccgtc gcctccaccg cctcctcatg cggcgagggc
     7081 ttccagccga gatcccgctg cgccttcgtg ctgcggtacg tccaccactg gccggcggcc
     7141 ttcacctcgt cgaccgagat cggcggccgg ccgggtgccg cgtcgagcgc ctgcgccagc
     7201 cgcaaggcca ggcccggcga gagcttcagc ggcggcggct cgcagccgga catgcgtgca
     7261 aggtccgcga aaaaccgatc ccacgtgtag ttgcggttgc cgaggatgta gcgctcgccc
     7321 accgcgccgc gctcgtcggc cagcaggtgc ccgcgtgcga cgtcacccac gccgaccacg
     7381 ttcagcccgc cctcgatgta caccgggata cgcccgagga gaaagcggcg caccacaccg
     7441 gtcgagccgc cggtcacatc gccgctgccg aacacgaagc aggggttcac gcacacgacc
     7501 ggcagtccct tggccgcgtg gcgcatcgcc tccacctcgg cctcgtgctt cgagttcacg
     7561 tatggaatct gtagatgccc ggccgtaaat agcgcgttct cgtcagcggt gccgccgcgc
     7621 gccgccgggc caatcgcggc gacactcgac gtgtacacca cgcgcacgac gcctgcgcgc
     7681 aagcactcct cgaacaggag gcgtgtggcc cccacgttca cctggaagag cacgtcggcg
     7741 tcggcgggcc gcagcgacag tagcccggcc gagtggaata cgcggtccac gcccttcagc
     7801 gcccgccgca ccgcgcggcg gtcgagcagg tcgcagcgca cgccctcgta gtcgagtccc
     7861 tcgagcccct cgagacgcga gtcctcgcgc agtgtgaggc gcaggtcgtc gccgcgctcg
     7921 gcgagcagcc gcgcgatgtg cgagccgatg aagcccgtcc ctcccgtcac gagggtcttg
     7981 cccacgggcg cgagtgtacg cgcgcgaatg ccgcttgccg actgctaggg cgcggcctcg
     8041 tgcgcctgct ggcatggcac gcagcgcacc gcctccggca cgagctcgag tcgcgcgggc
     8101 gcgatggtcg tcccgcacgc gacgcaaacg cgcgtccggc gcgcgcggtg tgcctcgatg
     8161 cgcgcgtgct cgccgccctc gacggcgtgt tcgtcggcat cctggctgcg gcgctcgcga
     8221 cggccgcgaa ccatgccatc agctcgctcg cgcagcagcg cttcgactcg atccctgtcg
     8281 cttccggtca gcatggagtc ctctccccgg ttgggcaggc cgcggcagcc ccgtccgcgg
     8341 ctctgtaggc tgcgcgcgca tgtcgcccgt cgcagcggtc acggacacta ccggttatct
     8401 gccggcggat gttgttcgcg acaactcgat tcacatcgtt tcgctgtatg tgaacttcgg
     8461 cggcgagcgc acggagcgcg aagcggacat caccgactac ggacgcttct tcgaagaact
     8521 gcgccgctcg gagcagctgc cgaccacgtc gcagcccgcg gtgggcgact tcatcggggt
     8581 ctatgagccg ctgctcacgc aggatcagga catcgtgtcc gtgcacatct ccggcggcct
     8641 ctcggggact gtcgacgccg cccgccaggc cgcccaggcg ctcgagggca aaggcggcga
     8701 gcgcgttcac ctgttcgact cggagaccgg cgcgggtggc acgggcatcc tcgcgctcgt
     8761 cgctgcgagg gcggcccaac gcggcgagtc cgccgagcag atcatggagc ggctgcgcgg
     8821 cgcgcgcgcg gacctgaaga tctggttcgc aatcgacacg ctcgagtatc tccgtcgtgg
     8881 cggtcgtatc ggcgcagcga gcgcctggat cgggtcgacg ctgaagatca agccgatcct
     8941 cacggtcgag cgcgagatga ctccggtcga gcgcgtgcgc acgagcaagc gcgcattcga
     9001 gcgcatggtc gactacgcca agcagcgcca ctcctcaggc gcggacgcct gggtcgtcca
     9061 gcacattcag tcgcccgacc aggcggctga attgaccgcg cgctgcacgg aaatcttcgg
     9121 caccggcccg ctgttcgtca gcgagatcgg tccggtgctc ggcgtgcaca caggaccggg
     9181 actgctcggc gtcggcggca ttccgccgca ctggctcgac tagcggcggc ggaagagccc
     9241 gaacgtggcg gcgatgccgc caccgatcac gaacccagcg acgccggcga cgatcatcgc
     9301 cgtctgcttg ttctgacgca gctgctgccg ccagtcggtg agctgctgga ccttcccctg
     9361 aagatcgttc agcgagtagg tgagctccgt gcgagtggcc tggaccgagc ggcgaatctc
     9421 atcgggcgag cgggtgggca tcagtgacgc gcctcctcga gagtggcgcg cgtcttcttc
     9481 gcctcctcga tcgcgagctc aggcacgggc ggcgctcctc tcttgaagaa gcgcaccgcg
     9541 agcaagccgg cgatcgcgcc gagaatcagt agcaaccccg cggtaattgc gaagccgggc
     9601 cacaccgaat tgaagtcgaa caggtcatcc cagagccacg acagcgcgtg gaagaagtag
     9661 atcagcacga acacgaggaa gaccccggca gccgccccga acgcggcgcc cttcgcgagc
     9721 cggttgatct tgacctggat ctcagcctgg gcgagctgga tctcctcacg aacgaggagc
     9781 gacgccttct cgctgacttc gttgacaatc tcgccgagcg acttctccgc gctgcctgga
     9841 ggcgcgccgt tgtggtcagt catctccgcc ccccaagcgc ttgagcacct gggcgaccaa
     9901 gaagccaccc gcaaacgccg cgcccacgag gatctcgggg cggtcgctgg actgctcgac
     9961 cgcacgcgtg tgcgcgtcca gcggggactg gcccgttgcg accgcgctgt acgaaccgcc
    10021 cgcggcggtc tcggcggcct cgcgccctag cgcgtcggtc tcggccgtgg gctgctcgga
    10081 cgcgctcgtg tgcgttgccg tgtcttcctg agtcatacgc tcactccggc gggtcctcgt
    10141 tgaatactcg ctgccagatg atggtcgcga tccggttcgc gacgatcgtg ctgagtgcac
    10201 cgatacccgc cagcagtcca ctccatgcga gccgcttgac catgtcgttg ctcatgaagc
    10261 gggcagtgta accgggcggg gcggctacga aaccggcgcc gatgccgagg gcgcctcgag
    10321 cccgccgcag atcgtctcga ccacgagacc ctttgcgcgc tcgaagtcct cgtcctgctg
    10381 cggcatgagg tcgaagatcc aacccgagag cagctgctcg atcgcgccgt agaagcacat
    10441 cgcggcgaag tccgaggaga tgtcggactt gaacgagccg tcgacgcgcg cggactccac
    10501 gatgccggcg atcagctggt acgcctcgcg gatcttctcg aggtgctggc gcccgaacga
    10561 gttcgccgcg cgcgtgacct cgacgatgat caccttcatc aggttcgggt cgtagcggta
    10621 ggagtcgatg atgaacgacg cgaccatgta cagcttgtcg cgcgccggga cctcctggcg
    10681 gtcgatgtcg acgatcgcgt cgagcatgat ctgccagcgc tcggtgaaga gcgtgttgag
    10741 gatctcctcc ttggaatcga agtagtggta cacgagcccg taggcgaccc cggcctcgtc
    10801 cgccacgtcg ctcacccggc aggcgtggaa gccctgctgc gcaaacacgc gcaccgcggc
    10861 gtcgaggatc agacgtcgct tgtcgacccc gttgggatgc tgccgcgtcg aggccatcag
    10921 gcgattgcct cttgcggcca gcggtaggcg gacgggacgc ggttcgcgag cgagggaagc
    10981 tcgcggcgca cctcgtcctg gcgctcgagg ttgaggtcgg ccgcgatgaa catctcctcg
    11041 tcgggcgcgc gtgcgagcac ctcgccccac gggtccacga tcatcgaacc gccaaagctc
    11101 tccttgccgt cgctcgtcgc gccgatctga tcggcggcca ccacgaaggc ctgattctcg
    11161 atcgcgcggg cgcgcaccag gatctcccag tgcgcccggc cggtcacctt cgtgaacgcc
    11221 gcgggcagcg tcagcacgcg cgcgccgcgc acggcgagga tgcggtagag ctcggggaag
    11281 cgcaggtcgt agcaaaccgt catcccgacg ggcacgcctt gcgcctcggt gatggtcatt
    11341 tcgtcgcccg gctcctcgga ctccgactcg cggtactcca cgccgccgac caccacgtcg
    11401 aacatgtgga tcttccggta gacgcccttc agctcgccgt cgggtccgat gtgcaccgac
    11461 gtgttcgaga gcttgtcgtg cccctcgcgc cgctcgacga tcgagccggc cacgaggtcg
    11521 atcccgagct cgcgtgccgt gtcgcgcgcc cagctgatcg tgggtccctc gagcgtctcc
    11581 gctcccgcga tgtagtcgtc gtgggtgcca agcacgttga acttctcggg cagcacgacg
    11641 agcgtcgcgc cgttcgcggc agcggcgcgc gtgaggcggt cggccgtccc caggttacgt
    11701 tccttgtctt ccgtggaatt gagctgaacc gctgcggctc gcacgcctgt tctcccccct
    11761 gccccgagtc ccattaaccg gtccccggtc ccggttgact gacgagtcaa tccaatctta
    11821 cctgccgggg tggtctaccc gggtcgacaa ccaacacccg tgatacttcg cgcggatggc
    11881 ctcagccggc aacctgcccg cgcgtggcgc gcgcgtgcga gacctcggtc tggcgctgcc
    11941 gcccggccgc atgccgctgc tccagcacgg ccgcccgctc aagcgctggc gctacgtggg
    12001 cgttttcggg ccggagctga tgctgtgcgc cggcgacgcg cgcgtagggc cgttcccgca
    12061 gcgctggtgg gcggtggcgc tgccggacgg gacgctgcgc gagcgcacga cggcgggtcg
    12121 tggcggcgtg atcatcgagc cgcggcgcct gcgcgtcgcg gcacccaacg cccgcatcaa
    12181 cgtgaacctc gacgaatcgg gaacagagct cgaggtggtc tcgccgagcg ggaagtccgg
    12241 ctacatctgg acgcgcaagc gcggcggggt tccagcgcgc ggccgggcgg tgatcgacgg
    12301 gcgcgtgctc gaagttgacg cgggcgcggt gatcgacgag agcgccggct accacgagcg
    12361 gcacacgagc tggcgctggt cggccggcgt cggccacacc gacacgggcg agccggtcgc
    12421 gtggaacctc gtggacggtg tgcacgacgc gcccgagcag agcgagcgca cgctctggat
    12481 cggcgacgag gcgcgcgagc tgccaccggt cgagttcgcg gcggacctgt cgcgagtggg
    12541 cggactgagc ttccgtgcct gggcgacgcg cgagcaccac acgaatctgt ggctgttccg
    12601 gtcggactac cggcagccat tcggcacatt cgagggcgag ctcgcgcccg gcgtgcgcct
    12661 cgccgagggc tggggcgtga tggaggagca cgacgcgtgg tggtgacagt cgagcggcgg
    12721 gccgcgcctc ctgcatcgcg gcgcggggcg cgcgagtcgc cggcgcttcc cgggcgggag
    12781 ctcgcgctcg ccgcgctggc ggccgtcctc atagcgtgcg tgatgcactg gccgatcccg
    12841 atcaacctcg ggagcgacgt gccgcgcgac ctcggcgatc cgctcacgca ggcgtggcag
    12901 gtcgcgtggg gcggccacgc cctgctgcat cagccgctcg actacttcca ggcgaacgac
    12961 ttttggccgc tgaaggaccc gctcgcgttc tcggacgcgc tcgtgggtta tgcgccggcc
    13021 gggatgatcg gcagcggtcc gcacgccgcc atcgtgcggt acgacctgct gttcctgttc
    13081 gcgtacgcgc tcgcgttcct cggcgcgtac ctgctggcgc gagagctcgg cgcgcgccca
    13141 atcccagcca cggttgccgg cgcggcgttc gcgtacgcgc cgtggcgcct cgagcaggac
    13201 gggcacctgc acgtgatctc gagcggtggg atcccgctcg cgctgttcct gttgctgcgc
    13261 ggctatcggc gagccaaccc cgtgaccgtc gccgccggct gggttgtcgt cacctggcag
    13321 atcagcctcg gcttcacgct cggccttcag ctgctctacc tgatctgcct gctcggcgcg
    13381 ggcgcagccg tgctgatctg gcgcaagcgc ctgccgctac cgccgcggcg cgtgcttgcc
    13441 gccaccgccg gcggtgttgc cctgacggcg gcggtgtcgg cggcgctggc ggtgccgtac
    13501 gcccgggtgc tcgaccgcca tcccgaggcg aagcgcacgc tcgctcaggt cagctcgctc
    13561 tcgccgccac tgaaggcgtt cgccgccgcc cctccgcaga acctgatctg gggcaaggcg
    13621 accgagggtg tgcgcaacga cgtgcgctgg gtgccggaga tgtcgctctt cccgggggtc
    13681 gcgatcctcg cactcgcgct aaccggactc ttctggcgtg gcgctccgcg agggctgcga
    13741 atcggcctgg gcgtgtccgc ggtcgtcgtc tggctgctgt cgctcggcat tcacggcggc
    13801 gggccctggc cctatcgact gctttacgag gttctgcccg gctggcaggg catccgcgtg
    13861 ccggggagac tcatgacgct cacctcgctc gcgctcgcgc tgctcgcggc gttcggcgcg
    13921 cagcggatcc tcgccgcgcg tcgcgtgccc gcggcagtcg gcgtgggcct cgtagtgctc
    13981 gtgctgctcg aggggtcggg cttaggcatc gatgaacgcg gtgccgccat cgcgggaccc
    14041 gcccatccca ccgttccgcc gcagccaagc ggccagatag gagcgcccgc cccgcaggcg
    14101 cacctgcccc tcgacacgct cgacagccgc aagtacctgc tctggtcgac cgaccgcttc
    14161 cccgagctcg tgaatgggcg ggggagcttc aggcccgctg agttcaagcg actccaggtc
    14221 gagctcgctt cgttcccgga cgcgcgcagc gtcgccgtgc tgcagcaact gcgtgtgcgc
    14281 acggtcatcc tccatcccac gtttgtgcgc ggcacgccgt gggccgcctg gcgcgcgcgg
    14341 ccggtcagcg ggttgccgct tcgccgcgag gagcgcggcg gcgtggtgct ctaccagctg
    14401 cttccgcgct gaacgctgcg gccaggagca cggcgagcag gcccgcgccc atcgacaccg
    14461 cgacgagcgc gagggcctgg cttgtgtgag ccacgtcgcc atcgccgcgg cctagaaaat
    14521 cgggcacggt cgcagcgacg ccggcccatg cggcaagcgc gagcgccgcg gtgagcacat
    14581 cgcggcgcac gattcgcatc ggccgggtgg cccacgctgc gagcactccg gcagcgacca
    14641 gcgccacgag gaacggcgtg atgccgagcc agccgcgccc gagaccaagc accgtgagga
    14701 acgtgttctg gaagctgtgc gcgccgacca gcttcaccca gtagccgacg tcctggttgc
    14761 cgatgagcgg caacgtgatc gtggccgtcg ccagggtgac cgtcgaagcg accgccaggc
    14821 cgagcacagc cgcgggccag cgcttgaacg cgagcgcgag cggcgcgcat aggaacggga
    14881 tcgccgggat caggaagcgc ggtccaggag agccgccgcc gaacgggagg tagtagcccg
    14941 cgttgtagac gaggtaggag agcccgaccg ccgcgaccac gagcgcctcg gcacgcttgc
    15001 cgctgcggta gagcaggacc gtgccgacga tgccgagcgc gagcagcggg ctcgtgatca
    15061 gcagcccctt cgccgagaac agcaggtcag cggccacctt caagctcggc gcgccgatcc
    15121 cgaagaagcc cgagctgttc agccccagca cgtcgtgacc actcgcgccc tggatagcca
    15181 cggcgtcggc gtagccggag tggagcggct gaccgaacgc ccagaggttg tagccgaaca
    15241 gcggcagcaa cccgacgacc gcgccgcctc cgtacgcgaa ggcgcgccgc agccggtcgc
    15301 gacgcgcgag cgcgtagagg ccgacgatga ccgcgaccag cccgagcggg tactcgcacg
    15361 tgatcgcgag cccggcgagc gcgcccgcca gggcgacgcg gccgaggtgc tgcggcccct
    15421 cgcgctccac ccacaggacg acgaacgccg caaatccgag gcaggctgag agcacgtgcg
    15481 aga
//
LOCUS       DASVXS010000197         5181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_239070, whole genome shotgun sequence.
ACCESSION   DASVXS010000197 DASVXS010000000
VERSION     DASVXS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239070"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..784)
                     /locus_tag="VF752_10945"
     CDS             complement(260..784)
                     /locus_tag="VF752_10945"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856108.1"
                     /translation="MRRIFAIALPFLAIPVIAAGCGGSSNANTGSSNASNMSSTSSSN
                     ASTSAGSAARSATVKLRKTSLGMMLVGPSGRSLYLFLKDKKGKSACYNACASGWPPLT
                     TKGKPTAGKGVKASLLTTVKRKDGTMQVVYNHHPLYYYAGDSKAGQTSGQGLNQFGAK
                     WYVLNAKGNKIDTD"
     gene            928..1995
                     /locus_tag="VF752_10950"
     CDS             928..1995
                     /locus_tag="VF752_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8856109.1"
                     /translation="MRVGLVLGAGGVLGGAWLVGGLHALATETRWDPASADTIVGTSA
                     GSMVGSLLASGVPPWFMVAHSAGEVFDGLVDAEGRPAEEADRSAGAVFQLERALPPIG
                     PGSWGLALRSAMQPHRHTPAAVLAGWLPRGVFSTEPLKDTIRRVVPDGWAEHPHLWVV
                     ACDYSTGRRVQFGRDGAPPADLADAVAASCAIPSFYHPVEIGGRRYVDGGLYSNSNLD
                     VLRAQELDLVVCLNPTSTLNPTHAYNPAEWLAGAARSLSGRRLGSEAKKLREAGTSVV
                     LIQPTAEDIEVMGPNLMSTRRRHEVIETAIRTVREQLREPGVRGLLRKLPPGQPERIA
                     RPAGPPSTWPLPGNTAYREAS"
     gene            1992..2921
                     /locus_tag="VF752_10955"
     CDS             1992..2921
                     /locus_tag="VF752_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8856110.1"
                     /translation="MSEGGATAEGLASRASQNGAGSARSLLEMTGRLAGALAGEFQRR
                     IPTADLDARDPDFIRERLPGSWLLASLYFRGEVRGLDNIPEEGPVLLVGNHSGGNLTP
                     DTTVFTLAFYTWFGVERAFYQLAHNLVVSWPGLGWLRKFGTVAASPENADRALSSGAA
                     LLVYPGGDYEVHRPSWESGKVDFNRRKGFIRLALAKGVPIVPVVGIGGQETALFLSRG
                     ERLARLLLLDKLLRLKVLPISLALPWGLNVGDFFGHFPLPAKITMQVLPPIEVAKEFG
                     RKPDLDEVYDHVVRQMQAALDVLAEERRLPVIG"
     gene            2918..5062
                     /locus_tag="VF752_10960"
     CDS             2918..5062
                     /locus_tag="VF752_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8856111.1"
                     /translation="MRVEECIVIDASPQEIWNVITDPERYREFMHGVTRWEVEGRKKR
                     GKGARYRLNMEVGSAQVGGLVEVVEFDEPRDMAWTSVLGIDQRGRWRLRETDDGRTRV
                     TLRLSYQSPSQILGFIADNVSAPIVRRNLRHSLRQLAADLEGRQLRGTDMQDAGLVKT
                     VTQRLWSAQVLARAGFLRLHRPDQLARALATLQRWGPTPAAGYKSAALLFPDEDAVID
                     EAGHLTFREVHERSNALANALADRGINEGDNVGLMLRNHRYFIDAIAALSKLGAHALL
                     LNTAFAGPQLTDVVQREKPVALIFDQEFYGLLEDAGKRRKRFVAWEDDPSEVKDPTVE
                     SLIRDGDTSDLVPPEEKGRVVILTSGTTGTPKGASRKQPDTVDPAAALLSKIPLRSRE
                     KWLIAAPLFHSWGFAHFTLGMLLSSTMVLQRRFDPEQTLAGIAQHQAVSCPMVPVMVQ
                     RIMELPEETRRKYDTSSLRAVPLSGSALPGELAERFMAEFGDIVYNLYGSTEVAWATI
                     ATPADLHVAPGTAGRAPRGTIVRIYDERGRALPPGETGRIFVGNEMLFEGYTGGGKKD
                     VIDGLMSTGDVGHLDESGRLFVEGRDDEMIVSGGENVFPAEVEDLLAKHEKIAEAAVI
                     GVDDEKFGQRLKAFVVTRNGASVSEDEVKSYVKANLARYKVPREVEFLDELPRNATGK
                     VLKRELADEGDGSDGSRPRSRRGSRAKSKTRS"
BASE COUNT          800 a         1715 c         1822 g          844 t
ORIGIN      
        1 gggacttgga agggaaggcc ggcgcacgcc cgggccgcgg tggggagggc tctgcgaccc
       61 gagcgctgga gacagccggc gatccatagg ttcgatggag tgtctaaagc agccttaaga
      121 ccgtggttgc aattcggtaa aacgccgacc gcgcactaca cacccgcgcg ccgcgtgttt
      181 caagggcgca ggctccgtcg tttcgaaccg ttcatgtctt gccgcacgag ttcgtgcgcg
      241 cgctggcgtg ctcgatccct tagtcggtgt cgatcttgtt gcccttggcg ttgagcacgt
      301 accactttgc gccgaactgg ttcagcccct ggcctgaggt ctggccggcc ttgctgtcgc
      361 cggcgtagta gtagagcggg tggtggttgt agaccacctg catcgtgccg tccttgcgct
      421 tcaccgtcgt gagcagcgat gccttcacgc ccttgcccgc ggtcggcttg cccttcgtcg
      481 tgaggggcgg ccatccggag gcgcacgcgt tgtagcacgc gctcttgccc ttcttgtcct
      541 tcaggaagag gtacagggag cggccgctcg ggccgacgag catcatcccg aggctcgtct
      601 tgcgcagctt cacggtcgcg gaacgagcgg ccgatccggc gctcgtcgac gcattcgagc
      661 tactggtgct gctcatgttg ctcgcgttgc tcgagcccgt gttggcgttg gaacttccgc
      721 cgcagcctgc cgcgataacc gggatggcca ggaacggcag ggcgatggcg aatatgcgtc
      781 tcatgtggtc ctcctgtgtt tgcccactgc aaaggaggac gctgcctcgc gccctgcacg
      841 ttcgtgcctt cctaaagagt tcctgagacg aagaactcct ggataggcga cacttacttt
      901 tcggtcagca atggacggac aggaaaaatg agggtcgggc tcgtgctcgg ggccggcggc
      961 gtgctgggtg gagcctggct cgtgggcggg ctgcacgccc tcgcgacgga gacgcgctgg
     1021 gacccggcgt cggccgacac gatcgtgggc acgagcgcgg gctcgatggt cggttcgctg
     1081 cttgcctccg gcgtgccgcc gtggttcatg gtggcccact cggccggcga ggtgttcgac
     1141 ggcctcgtgg acgccgaggg ccgtccggcg gaggaggccg accgctcggc cggcgcagtc
     1201 ttccagctcg agcgtgcact gccgccgatc ggcccgggtt cgtggggact ggcgctccga
     1261 agcgccatgc agccccaccg gcacacaccg gcggctgtgc tcgcgggctg gctgccacgc
     1321 ggcgtgttct cgaccgagcc gctcaaggac acgatccggc gcgtggtgcc tgatggctgg
     1381 gccgagcatc cacacctctg ggtcgtggcc tgcgattact cgaccgggcg ccgcgtgcaa
     1441 ttcgggcgcg acggagcccc gcccgccgac ctggccgacg cagttgcggc atcgtgcgcg
     1501 atcccgagct tctaccaccc ggtcgagatc ggcggccggc gctatgtgga cggcgggctc
     1561 tactcgaact cgaacctcga cgtgctgcgt gcccaggagc tcgacctcgt cgtttgcctg
     1621 aacccgacgt ccacgctcaa cccgacgcac gcgtacaacc ctgccgagtg gctcgcgggt
     1681 gccgctcgct cgctttccgg tcgccgtctc ggatccgagg ccaagaagct gcgggaagcg
     1741 ggtacgagcg tggtgctgat ccagcccacc gcagaggaca tcgaagtcat ggggccaaac
     1801 ctgatgagca cccggcgcag gcacgaggtg atcgagaccg cgatccggac ggtccgcgag
     1861 cagctgcgcg agcccggcgt ccgcggcctc ctccgcaagc ttccgccggg gcagcccgag
     1921 cggatcgcgc gcccggccgg tcccccctcg acctggccgc tgcccggcaa taccgcctac
     1981 cgggaggcgt catgagcgag ggaggggcga ccgcagaagg tctcgcgtcg cgggcctcgc
     2041 agaatggcgc cggctccgcg cgctcgctac tcgagatgac cggccgcctg gcgggggcgc
     2101 ttgccggcga gttccagcgc cggataccca cggccgacct cgacgcgcgc gaccccgact
     2161 tcatccgcga gcggctacca ggctcctggc tgctcgcgag cctctacttc cgcggcgagg
     2221 tgcgggggct cgacaacatc ccggaggagg gcccggtcct gctcgtgggc aaccactcgg
     2281 gaggcaacct aacgcccgac acgaccgtct tcacgctcgc gttctacacg tggttcggcg
     2341 tcgagcgggc cttctaccag ctcgcccaca acctcgtggt gtcgtggccg gggctcggct
     2401 ggcttcggaa gttcggcacg gtcgcggcct ctccggagaa tgcggaccgc gcgctctcat
     2461 ccggggcggc gctgctcgtc taccccggcg gcgactacga agtgcaccgg ccgtcgtggg
     2521 agtcgggcaa ggttgacttc aaccgccgca agggcttcat ccgcctagcg ctcgcgaagg
     2581 gcgtcccgat cgtcccggtc gtgggcatcg gcggccagga gaccgcgttg ttcctctcgc
     2641 gcggcgagcg gcttgcccgg ctgctcctgc tcgacaagct gcttcgcctg aaggtgctcc
     2701 cgatatcgct ggcgctgccg tgggggctga acgtaggcga cttcttcggc cacttccccc
     2761 ttccggcgaa gatcacgatg caggttttgc cgccgatcga agtggcgaag gagttcgggc
     2821 ggaagccgga tctcgacgag gtctacgacc atgtggtgcg ccagatgcag gcggctctcg
     2881 acgtgctcgc ggaggagcgg cggctgccgg tgatcggatg agggtcgagg aatgcatcgt
     2941 catcgacgcc tctccccagg agatctggaa cgtcatcacc gaccccgagc gctaccgcga
     3001 gttcatgcac ggcgtgacgc gctgggaggt cgagggacgc aagaagcggg ggaagggcgc
     3061 gcgctaccgc ctgaacatgg aggtcggatc cgcccaggtg ggcgggctgg tagaggtcgt
     3121 cgagttcgac gagccgcgag acatggcgtg gacgagcgtg ctcggcatcg accagcgcgg
     3181 gcgctggcgg ctgcgcgaga ccgatgacgg gcggacgcgg gtaacgctcc ggctcagcta
     3241 tcagtcgccg agccagatcc ttggcttcat cgctgacaac gtgtcggcgc cgatagtccg
     3301 ccgcaacctg cggcattcac tccggcaatt ggcggcggat ctggaaggta ggcaactcag
     3361 ggggacagat atgcaagatg ccggtttggt gaagacggtc acgcagcgtc tgtggagcgc
     3421 gcaggtgctg gcgcgcgcgg ggttcctgcg actgcaccgg cccgatcagc tcgcacgcgc
     3481 gctcgccacg ctgcagcgct ggggaccgac gcccgccgcc ggctacaaga gcgccgcgct
     3541 gctcttcccc gacgaggacg ccgtgatcga cgaggccggt cacctcacct tccgtgaagt
     3601 acacgagcgc tcgaacgcgc tggcgaacgc cctcgcggac cgcgggatca atgagggcga
     3661 caacgtgggc ctgatgttgc gaaaccaccg gtacttcatc gacgcgatcg cggccttgtc
     3721 caagctcggg gcccacgcgc tcctgctcaa caccgcgttc gccgggccgc agctcaccga
     3781 tgtggtgcaa cgcgagaagc ctgttgcact gatcttcgac caggagttct acggactgct
     3841 agaggacgcc ggcaagcggc gcaagcgctt cgtagcgtgg gaggacgatc cgagtgaggt
     3901 gaaggacccg accgtcgagt cgctaatccg cgacggcgat acgtccgacc tggtcccacc
     3961 agaggagaag ggccgcgtgg tgatcctcac ctcgggcacg actggcacgc ccaagggcgc
     4021 ctctcggaag cagccggaca ccgtggatcc ggcggcggct ctcctctcga agatcccgct
     4081 ccgctcgcgc gagaagtggc tgatcgcggc gcccctgttc cactcctggg gcttcgcgca
     4141 cttcacgctc ggcatgctgc tttcgtcgac gatggtcctg cagcgccgct tcgacccgga
     4201 gcagacgctc gcggggatcg cgcaacacca ggccgtctcc tgcccgatgg tgccggtgat
     4261 ggtgcagcgg atcatggagc tcccggagga gacgcgccgc aagtacgaca cctcgtcgct
     4321 gcgggctgtg ccgctgtcgg gctccgccct gccgggcgag ctggccgagc gcttcatggc
     4381 cgagttcggc gacatcgtct acaacctcta cggctcgacc gaggtggcgt gggcgacgat
     4441 cgccaccccc gccgacctgc acgtcgcacc gggcacggcc ggccgcgcgc cgcgcggcac
     4501 gatcgtccgc atctacgacg agcgggggcg cgcgcttcct cctggcgaga cgggccgcat
     4561 cttcgttggc aacgagatgc tgttcgaggg ctacacaggc ggcggcaaga aggacgtgat
     4621 cgacgggctg atgagcacgg gcgacgtggg ccacctcgac gagtctgggc gcctgttcgt
     4681 ggagggccgc gacgacgaga tgatcgtgtc gggcggcgag aacgtgttcc ccgccgaggt
     4741 ggaggacctg ctcgcgaagc acgagaagat cgccgaggcg gccgtgatcg gcgtggacga
     4801 cgagaagttc ggccagcgcc tgaaggcgtt cgtggtgacg cgcaacggcg catcggtcag
     4861 cgaggacgag gtgaagtcct acgtgaaggc gaacctcgcg cgctacaagg tgccgcgcga
     4921 ggtcgagttc ctcgacgagc tgccgcggaa cgccacgggg aaggtgctca agcgagagct
     4981 ggccgacgag ggcgacgggt cggacggctc ccgcccgcgg tcccggcggg gctcccgcgc
     5041 gaaatcaaaa acacgctctt agggagtgct cgagaggggg tgggtgacct ttgggtcgct
     5101 attcgaccct aacctcaccc acgcctagat cgagctagcc cagctgaggg agttcactct
     5161 ttattgccgc tgttcacgcg g
//
LOCUS       DASVXS010000198        13151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_875568, whole genome shotgun sequence.
ACCESSION   DASVXS010000198 DASVXS010000000
VERSION     DASVXS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875568"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /gene="hpnC"
                     /locus_tag="VF752_10965"
     CDS             complement(<1..675)
                     /gene="hpnC"
                     /locus_tag="VF752_10965"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX8856112.1"
                     /translation="MGTTAPAPTEVMAKAGGENFPVASRLLPRREREHLLAVYGFARL
                     VDDAGDEASGDRRALLDELDADLARVYEGEPRHPIMRRLQPTVRACAIPREPFERLIE
                     ANRRDQRVHRYETFEELLEYCTFSADPVGEIVLHVFGAATPERIELSNAVCSGLQLAE
                     HWQDVAEDRGRGRVYVPSEDMRRFGVAEEELDGSRASEAFRRLMAFEVERARSLLDRG
                     APLIRTL"
     gene            complement(693..2579)
                     /gene="shc"
                     /locus_tag="VF752_10970"
     CDS             complement(693..2579)
                     /gene="shc"
                     /locus_tag="VF752_10970"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX8856113.1"
                     /translation="MAVRAEPAAHADAQEALERAQEHLLGLQQEAGFWRAELETNPTM
                     DAEDLLLREFLGIRDEGKLERAAKWIRSQQRPDGSWSNFYGGPSNLSTNVECYAALRL
                     AGDPVDAPHMLAAAERIREEGGIEQARVFTHIWLALFGLWDWEDVPALPPELGFLPKW
                     APLNLYDFACWARQTIAALTIVRSYRPVRPLPFDLDELRVPAGPRQLRPLTTMKGLLE
                     RLDLIARRYERRPVKWVRRLALARMERWVLTRQEADGSWGGIQPPWVYSLMALAVRGY
                     SLDHPAMRAGIDGIEGFIIEEGDTRRLEACQSPVWDTALAIIALADSGLDRSDPALVR
                     AADWILREEVRVRGDWAVRRPSLAPGGWAFEFENDNYPDVDDTTEVVMALRRVQHPER
                     SRVEAAIERALDWTIGMQSKGGGWGAFDVDNVRELCRQIPFCDFGEVIDDPSADVTAH
                     ALEMLAGEGLMRHPAAQRGLEWLLGEQEADGSWFGRWGVNHVYGVGATVPALVEMGIE
                     GSHPSIRRAVAWLEQVQNEDGGWGEDCRSYIDRAWIGRGESTPSQTAWALLALHAAGE
                     RDSVAVERGLAYLVATQTEDGTWEEPFYTGTGFPGDFYINYHLYRLVFPLTALGRLLR
                     DEED"
     gene            2741..3394
                     /locus_tag="VF752_10975"
     CDS             2741..3394
                     /locus_tag="VF752_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8856114.1"
                     /translation="MTTQTPKMSRAELLRASPPMFESKLLDRLSRVHPAVPVIIFAPA
                     ITAFFVVAAINMSALRVIGYAALGYLIWSLSEYWIHRIIFHFEPEDGLGARLHWIIHG
                     VHHDHPNDPLRLVMPPSVSVPLSTGFFFAFWGIFGLPIAWAVAAGFFLGYLAYDMTHY
                     HLHHHTSRTRLGRYLRELHMRHHFQDHTRGYGISAPYWDRVFGTWLDRSKRAERAPE"
     gene            3507..3590
                     /locus_tag="VF752_10980"
     tRNA            3507..3590
                     /locus_tag="VF752_10980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3541..3543,aa:Leu,seq:cag)
     gene            complement(3800..4189)
                     /locus_tag="VF752_10985"
     CDS             complement(3800..4189)
                     /locus_tag="VF752_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856115.1"
                     /translation="MSQQNVAIARRWVELFNTRDDVDEFLSLHDPEVVLQTPGGPRLR
                     GHDQLRDWFEAGYEHVQPRIVPERFVAEGDTVVGLGRTEARWIESGDVAHEGESAAAY
                     WFRDGKIIRWQPFETHAAALKETGLAE"
     gene            4253..4594
                     /locus_tag="VF752_10990"
     CDS             4253..4594
                     /locus_tag="VF752_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856116.1"
                     /translation="MSSLGEHAPQNAMDDPGEIARFYDRCSDLMRELLEALAHAPDRP
                     RPFPQIEDEIGWPHRRIASVLGGVSHLRHTEFGGRRPYHFHDQHQAESARWEMWMDAA
                     QARTVREARRG"
     gene            complement(4629..5048)
                     /locus_tag="VF752_10995"
     CDS             complement(4629..5048)
                     /locus_tag="VF752_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856117.1"
                     /translation="MPAIRRRLVLIVLAACAALALVASTGFGRGDNHRAHITRSGAVF
                     GKQVVSAASPAGFVYVRANGTLAPGTYGQIVLTCPHKFPHPVSGLFDTNNDFTLHATS
                     RPDPDGVSARTAHKWAEGVTNTSQTQAGVTVGAVCMK"
     gene            5149..6441
                     /locus_tag="VF752_11000"
     CDS             5149..6441
                     /locus_tag="VF752_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856118.1"
                     /translation="MLGPQTRRELLRNGGIAIGVVGAARLGWLPADARATSAPLRRCV
                     SLGANGVINPRSSQDYRRNRTFLLDTGTTWVRLWADWPSLQPEPSLAPDRGSGAWRLA
                     ELDKQIAQANADGVHVILCSYRFPTWANGTADLSPSEEVTYELPDRASAAGTSRKDLR
                     FKLPENLGPTSAWAAWIRFLVKRYGPGSPSRKPTIAALEVCNEPNGQMWPLQRPSTTA
                     DAYTDGPLTVQTAVAQMFITAQGITAEFGSQPVLMGPATADSTGDSRLAVSYDTFTGA
                     LLDELTRLGFRAGPQFAWTHHNYTDVESDLGSGSWTGDLANGAAAVREMLRGRWAGWP
                     SADASQPRVFMTEGGARLSEIANVYGSSDREFLLQRQAELIQRNWARMRSEREGAGIA
                     MFAQYLFGTDASFDSGLCDPSGVKRPAYDAWKALPLSA"
     gene            6513..7013
                     /locus_tag="VF752_11005"
     CDS             6513..7013
                     /locus_tag="VF752_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8856119.1"
                     /translation="MFDKIENPKELFGYKLGAAYTMEQNVLGMLDDLQQETTNDQLRQ
                     LFSHHADETRQQIGNIEQAFAALGEEADDKPCPAIQGLEKESKAEIKMAEDSVVDAVI
                     LSGAAETEHHEIAVYESLITQAEAMGNQQVAQLLKQNLEQEQHTLEEVKGESQRLAQT
                     MAGSTA"
     gene            complement(7024..7476)
                     /locus_tag="VF752_11010"
     CDS             complement(7024..7476)
                     /locus_tag="VF752_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX8856120.1"
                     /translation="MDLLNAADSSWPILNRVFSGHAFVYRASGGWVGRKLPGLPPFLL
                     LEHVGAKSGAKRVSPLVYVEDGVDIVLVASKGGYPRNPAWFHNLRANPDSTIQIGRER
                     RPVRAQVARPEERERLWPMAVEVYGGYHGYQERTEREIPLVILEPRES"
     gene            7562..8482
                     /locus_tag="VF752_11015"
     CDS             7562..8482
                     /locus_tag="VF752_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8856121.1"
                     /translation="MSPELRHLRYFVALAEELNFTRAAERLHVAQQTLSSTIQQLERE
                     LGVQLFERTTRRVQLTDAGRRLIEDAGGALGAVESAWRHARGDGDAAAELRVAYSHTI
                     GPRCVPALHEATRRVLPDVRVTWWEMWASDIPQAVSSGTYAIGLARFPVIDADLECET
                     VAEEEQGILVSSRHPYAGFDKLRLAELGHETFLVFPREMAPGYFDAIVRMLRTFGGFE
                     PRLMVSPDAGDDGIIPAIYESGSALGLAPSSSGRLFAERSGGRIVLVALDQPAPLAPI
                     DLVRRPVAPDPTVESLVRLVRMLGKRGQLA"
     gene            8589..9149
                     /locus_tag="VF752_11020"
     CDS             8589..9149
                     /locus_tag="VF752_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8856122.1"
                     /translation="MRHRPHAESTPGAPNLRDPSQFDAAVRDLRPAVLRWARIVLRDD
                     AAAEDLAQDLFTQLWLNPEAYDSSRTSLRSYLRVLTRSRAIDRFRTRGAHQSAAQRLA
                     VEQLSHPRAAESPEAPALRREVARQLLHALDELPREQRAAVLLAAVGELTASELALAS
                     EIPLGTAKSRIRIGLSKMRGVLDEAA"
     gene            complement(9146..10351)
                     /locus_tag="VF752_11025"
     CDS             complement(9146..10351)
                     /locus_tag="VF752_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8856123.1"
                     /translation="MIALGAALVIAVAALPHEHDRRYSVAIVYLGLGALSGAGLRLFD
                     RGPLDPVGDQLLLRRASEAAMALALFATGLRLRRRLSPRAWASPARLLGIAMTLTIAA
                     VAAWAAGPMGLTLGAAIALGAALAPTDPVLAGGLGVGPPGAAEEEREAEFTLSAEAGF
                     NDGLGVPFVVLGTLVATKGGTSWLGEWIGTEVLYGAGAGIVLGGLAGYLFGKGTFALR
                     RRGLLVPELDPWLGPAGAVLIYGAAQALDLYGFLAVLAAGIGFRHQELETSYNRRVHD
                     GAEVVKTFAELVVLALFGAMLTWGDFGAPGVSGWLLVPLLIFVIRPVLVFVSLVGDDR
                     LSLRDRIYLAWFGVKGIASLNYLALIVASGAFAGAELEQIVWTIMAVVLVSIVVHGLS
                     ATPLVKRLR"
     gene            complement(10411..11313)
                     /locus_tag="VF752_11030"
     CDS             complement(10411..11313)
                     /locus_tag="VF752_11030"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX8856124.1"
                     /translation="MSTLELYRTPEERFRGLPGYDFDPHYVDVDGLRMHYVDEGPSEG
                     EAIVCFHGEPTWSYLYRKMIPPLVDAGYRVIAPDYAGFGRSDKPLDRDWYGYDRHVDH
                     VSRVLDTLDLRRATVVVQDWGGPIGLRWAVENEARVGGLAILNTGLFTGRVSKGFMAW
                     RDFAERNPDMPVGFVVAGATATEVPPEVLAAYEAPFPTPESKAGAAQFPLIVPTSDED
                     AGAAEMRAVADALSRFEKPALVAFSDSDPVFPFPRSGQVFCDLLPGAGEQVRIEGAAH
                     FLQEDKGELIAEHMLEFLRGGGTG"
     gene            complement(11310..11969)
                     /locus_tag="VF752_11035"
     CDS             complement(11310..11969)
                     /locus_tag="VF752_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX8856125.1"
                     /translation="MEVSPAVTPIKPRLRGVTHQWAFFVSLVAGSALLVLAPGRKAAL
                     AAAIYAVSLSALFGTSALYHRITWRPSARRWMRRLDHSMIFLLIAGTYTPFCLLVLRG
                     GIANTVLAVVWGGALGGILLKLLWIDAPKWLVAAVYVALGWVAVAATPQILDHAGAVA
                     LGLVLAGGLLYTAGAVVYAVRRPDPVPAVFGYHEVFHALVIGAAALHYAAVAFFALPG
                     G"
     gene            complement(11983..12843)
                     /locus_tag="VF752_11040"
     CDS             complement(11983..12843)
                     /locus_tag="VF752_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856126.1"
                     /translation="MTRPEMPEPPVELPEGRIVHVHGTGEMFLRDTGGDGPPVLLLHG
                     WMFQSDLNWFRTYEPLAQAGYRVLATDHRGHGRGLRPAEPFSLEGCAADAAALVRHLG
                     CGPVTAVGYSMGGPISQLMARDHGDTVSGLVNCATAATWVEPRMRRLWKSMGALRLGL
                     GLFPQGAWRAGLRAGGFPDSPATTWVASELSRGSSLDIAEAGRELGRFDSRAWARSLD
                     KPAAVVVTSRDRGVPARRQHELADLLGAVRFEIPADHSAVIVNADEFSAVLLEAIGAV
                     RADQRIAAAS"
     gene            complement(12840..>13151)
                     /locus_tag="VF752_11045"
     CDS             complement(12840..>13151)
                     /locus_tag="VF752_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856127.1"
                     /translation="FARTFMIEVLAAGPAALERRAEVHRRFADRVRALTARAREQHPE
                     LPEPPPEVFSAIVGAVHELTWERVRLGQFDKLVELEPVISYIEIALVAGHDEAQGELG
                     R"
BASE COUNT         2056 a         4700 c         4514 g         1881 t
ORIGIN      
        1 cagcgtgcgg atcagcgggg cgccgcggtc gagcagcgaa cgcgcgcgct ccacctcgaa
       61 cgccatcagc cgccggaacg cttcgctcgc gcgcgagccg tcgagctcct cctccgccac
      121 gccaaagcgg cgcatgtcct cgctcggcac gtacacgcgc ccgcgcccgc gatcctcggc
      181 cacgtcctgc cagtgctcgg ctagctgcag gccgctgcag acggcgttcg agagctcgat
      241 gcgctccggc gtcgccgcgc cgaacacgtg cagcacgatc tcgccgaccg ggtccgctga
      301 gaacgtgcag tactcgagca gctcctcgaa ggtctcgtag cgatgcacgc gctggtcgcg
      361 ccggttggcc tcgatcagcc gctcgaacgg ctcgcgcggg atcgcgcacg cgcgcaccgt
      421 cggctggagg cggcgcatga tcgggtggcg cggctcgccc tcgtacaccc gggcgagatc
      481 cgcatcgagc tcgtcgagga gcgcgcgccg gtcgccagac gcctcgtcgc cggcgtcgtc
      541 caccaggcgc gcgaagccgt agaccgccag taggtgctcg cgctcgcggc gggggagcag
      601 gcgactcgca accgggaagt tctcgccacc ggccttcgcc atcacctcgg tcggcgcggg
      661 agcggtcgtg cccattgcgc ccaccctact gatcagtctt cctcgtcccg aaggagtcgc
      721 ccgagcgcgg tgagcgggaa caccagccgg tacaggtgat agttgatgta gaagtcgccg
      781 gggaagccgg tgcccgtgta gaacggctcc tcccacgtgc cgtcctcggt ctgcgtcgcg
      841 acgagatacg cgagcccgcg ctcgaccgcc accgagtcgc gctcaccggc ggcgtggagg
      901 gcgagcagcg cccaagcggt ctgtgagggc gtgctctcgc cgcggccgat ccaggcgcgg
      961 tcgatgtagg agcggcaatc ctcgccccag ccgccatctt cgttctggac ctgctcgagc
     1021 caggcgaccg cgcggcggat gctcgggtgc gaaccctcga tccccatctc cacgagcgcg
     1081 ggcaccgtgg cgccgacgcc gtacacgtgg ttcacccccc agcggccgaa ccacgacccg
     1141 tcggcttcct gctctccgag cagccactcg aggccgcgct gggccgccgg atgccgcatc
     1201 agtccctcgc ccgcgagcat ctccagggcg tgcgcggtca cgtcggcgct cgggtcgtcg
     1261 atcacctcgc cgaagtcgca gaatgggatc tgacggcaga gctcgcgcac gttgtcaacg
     1321 tcgaacgctc cccagccgcc gcccttgctc tgcatcccga tcgtccagtc gagcgcgcgc
     1381 tcgattgcgg cctccacccg cgagcgctcc gggtgctgga cgcgacgtag cgccatgacg
     1441 acctcggtgg tgtcgtcgac gtcggggtag ttgtcgttct cgaactcgaa cgcccagccg
     1501 ccgggcgcga gcgagggacg gcgcacggcc cagtcgccgc gcacgcgcac ctcttcccgg
     1561 aggatccagt cggcggcgcg cacgagggcg gggtcgctgc gatcgaggcc agagtcggcg
     1621 agcgcgatga tggctagagc ggtgtcccac accggcgact ggcacgcctc gagccggcgc
     1681 gtgtcgccct cctcgatgat gaagccctcg atcccgtcga tgccggcccg catcgccggg
     1741 tggtcgagcg agtagccgcg caccgcgagt gccatcagcg agtacaccca tggcggctgg
     1801 atgccgcccc acgagccgtc ggcctcctgc cgcgtcagca cccagcgctc catgcgcgcc
     1861 agagcgagcc tgcgcaccca cttgaccggg cgccgctcgt agcggcgcgc gatcaggtcg
     1921 aggcgctcga gcaggccctt catggtggtc agcggccgca gctgccgcgg tcctgccggc
     1981 acgcgcagct cgtcgaggtc gaacggcagc ggccgcaccg gccggtacga gcgcacgatc
     2041 gtcagcgcgg cgatggtctg acgcgcccag caggcgaagt cgtagaggtt cagcggcgcc
     2101 catttgggca agaagccaag ctcgggcggg agcgccggaa cgtcctccca gtcccagagt
     2161 ccgaacagcg cgagccagat gtgcgtgaac acgcgcgcct gctcgatccc gccctcctcg
     2221 cggatgcgct ccgcggcggc gagcatgtgg ggcgcgtcca ccgggtcgcc ggccaggcgc
     2281 agtgcggcgt agcactcgac gttggtcgag aggttcgaag gcccgccgta gaagttcgac
     2341 cagctgccgt cggggcgctg ctgcgagcgg atccacttcg cggcgcgttc gagcttgccc
     2401 tcgtcgcgga tcccgaggaa ctcgcggagc aggaggtcct cggcgtccat ggtggggttc
     2461 gtctcgagct cggcgcgcca gaagcctgcc tcctgctgca gcccgagcag gtgctcctgc
     2521 gcgcgctcga gggcctcctg cgcgtccgcg tgcgcggccg gctctgccct aacggccatt
     2581 tctcgacggc cccagttcga atgactgcgt caaaaatccg ccttcctgcc gatgcggcga
     2641 cagactaaac agtgcgtaca ccagcggacg ccttccgata gcccaaggtc ggctcgctga
     2701 cgccacgaat cagcggacgg tcgccgttac actggtgcgc atgaccaccc agacgcccaa
     2761 gatgagccgc gccgagctcc tcagggcctc gccgccaatg ttcgagtcga agctgctcga
     2821 caggctctcg cgcgtgcatc ccgcggtgcc ggtgatcatc ttcgcgccgg cgatcaccgc
     2881 gttcttcgtc gtcgcggcga tcaacatgag cgcgctgcgg gtgatcggct acgccgcgct
     2941 cggctacctg atctggagcc tgtcggagta ctggatccac cgcatcatct tccacttcga
     3001 acccgaggac gggctcggcg cgcgcctcca ctggatcatc cacggcgtgc atcacgatca
     3061 cccgaacgat ccgctgcgcc tggtcatgcc gccgtctgtg agcgtgccgc tctcgaccgg
     3121 cttcttcttc gccttctggg gcatcttcgg gctgccgatc gcgtgggcgg tggccgcggg
     3181 cttcttcctc ggctacctcg cctacgacat gacgcactac cacctgcacc accacacctc
     3241 taggacccgg ctgggcaggt acctgcgcga gctccacatg cggcatcact tccaggacca
     3301 cacgcgcggc tacgggatca gcgcaccgta ctgggacagg gtgttcggta cgtggctcga
     3361 ccgctcgaag cgggccgagc gcgcgccgga gtagcgcggc gtatcgccgt accgtggtcg
     3421 atcgggctcg atatccgagc ccaaaggacc acgctcgcgc gagagcgggc acgcgcgcgc
     3481 cgctcggcta tcctcgccat tcgccggcgg atgtggcgga attggcagac gcgcaggctt
     3541 caggtgcctg tgggggcaac cccgtggagg ttcgagtcct ctcatccgca tcgggcggct
     3601 gcaaagcttc ggattgcggt ccccgctcgc cccttgtggg ctgaagccca ccacggggct
     3661 cgcgtgaacc gcactcctcg cttttcgccg ccctatcggg gtctcgtgga ccgcgcgttc
     3721 gaagctccat ggctttcagt tgccatgctc ggccggcgcg gtaaacggtt ttgttctcgc
     3781 ttccgcaagc gagttgggct tactcggcta gtcccgtctc tttgagggcg gcggcgtggg
     3841 tctcgaacgg ctgccatctg atgatcttgc cgtcgcgaaa ccagtaggcc gccgcgctct
     3901 cgccctcgtg ggcgacgtca cccgattcga tccaccgggc ttccgttctc ccgaggccga
     3961 ccacggtgtc gccttctgcc acgaagcgct ccgggacgat gcgtggctgc acatgctcat
     4021 atccagcctc aaaccaatcg cgcagttggt cgtgcccgcg cagacgaggc ccgcctgggg
     4081 tttgaagcac aacctcggga tcgtgcaacg agagaaactc gtcaacgtcg tcccgcgtat
     4141 tgaagagttc gacccatcgg cgcgcgattg cgacgttctg ttgcgacacg ttccgcacac
     4201 tatctccggg cggaaccagc ctctccctcc gcggtcgcta gcgtcggccc ccatgtcgtc
     4261 actcggcgag cacgcgccgc agaacgctat ggacgatccc ggcgaaatcg cccgcttcta
     4321 cgaccgctgt agtgacctca tgcgcgaact gctcgaggcg ctcgcacacg cacccgatcg
     4381 gccccgtccg ttcccgcaga tcgaggacga gatcggatgg ccgcaccgcc ggatcgcgtc
     4441 cgtgctcggc ggcgtctccc acctccgcca caccgagttc ggcggtcgcc ggccctacca
     4501 cttccacgac cagcaccaag ccgagtcggc ccgctgggag atgtggatgg atgcggcgca
     4561 ggcgcggact gtgcgcgagg ctcgtcgcgg ttagccctcg gcgtggcgcg gaccccccag
     4621 gccgtgccct acttcatgca aacggctccg acggtcacgc ccgcctgtgt ctggctcgtg
     4681 ttcgtgactc cctccgccca cttgtgcgcc gtgcgcgcgc tcactccatc ggggtccggg
     4741 cgggacgtcg cgtgcagcgt gaagtcgttg ttcgtgtcga acagacccga cacggggtgc
     4801 gggaacttgt gtgggcaggt gagcacgatc tggccgtacg tgcccggcgc aagcgtgccg
     4861 ttcgcgcgca cgtagacgaa gcctgccggg gaggcggcgc tcaccacctg cttgccgaac
     4921 acggctccgg acctggtgat gtgcgcacgg tggttgtcgc cacggccgaa gccggtagac
     4981 gcgacgagcg ccagcgcggc gcaagccgcg agcacgatca ggacgagcct tctgcgaatg
     5041 gcgggcatgg accagacctc cttctgaggg gcgaccagaa acgcgtgtgc gcgaccgtat
     5101 gcggtgagtg gtcacggcgc aataccgtct ggaggagagg aggtagacat gctcggcccc
     5161 cagactcggc gtgagcttct gcgcaatggc ggcatcgcca tcggcgtggt cggcgccgcg
     5221 cggcttgggt ggctcccggc cgacgcccgc gcgacaagcg ctccgctccg tcgctgcgtg
     5281 agcctcggcg cgaacggggt gatcaacccg cgcagcagcc aggactatcg ccgcaaccgc
     5341 accttcctgc tcgacacggg cacgacatgg gtgcggctgt gggcggactg gccgagcctc
     5401 cagccagagc cgtcgctggc accggaccgc ggcagcggcg cctggcggct cgccgagctc
     5461 gacaagcaaa tcgcgcaggc gaacgccgac ggcgtccacg tgattctgtg ctcgtaccgc
     5521 ttcccgacct gggccaacgg gaccgcagat ctctccccct cggaggaggt cacgtatgag
     5581 ctgccggacc gcgcgagcgc ggctggcacg agccgcaagg atctccgctt caagcttccc
     5641 gagaacctgg ggccgaccag cgcgtgggcg gcgtggatcc gctttctcgt gaagcgctac
     5701 ggccccggct cccccagccg gaagccgacg atcgcggcgc tcgaagtgtg caacgagccg
     5761 aacggccaga tgtggccgct tcaacggccg agcacgaccg cggatgccta taccgacgga
     5821 ccgctcaccg ttcagaccgc cgtcgcgcag atgttcatca cagcgcaggg catcacggcg
     5881 gagttcggct cacagccggt gctcatgggc cccgccaccg cggacagcac gggcgactcg
     5941 cggctggcgg tgagctacga caccttcacc ggtgcactcc tcgacgagct cacgcgcctc
     6001 ggcttccggg ccggtccgca gttcgcctgg acacaccaca actacaccga cgtggagtcc
     6061 gacctgggct caggctcatg gaccggcgac ctcgcgaatg gcgcggccgc ggtacgcgag
     6121 atgctgaggg gccgctgggc cggctggccg agtgcggacg cgtcgcagcc gagggttttc
     6181 atgaccgagg gcggtgcgcg ccttagcgag atcgcgaacg tgtacggaag ctcagaccgg
     6241 gagttcctgt tgcagcggca ggccgagctg atccagcgca actgggcccg catgcgcagc
     6301 gagcgcgagg gcgccgggat cgcgatgttc gcgcagtacc tgttcggtac cgacgccagc
     6361 ttcgactccg ggctgtgcga cccgagcggc gtcaagaggc ctgcctacga cgcatggaag
     6421 gcgcttccgt tgtccgcctg acccgccccg tttcgccgca tcccgcctgg gtagcccatg
     6481 agggtcatgg catcgacccg ttaaggaacg gcatgttcga caagatcgag aaccccaagg
     6541 agctgttcgg ctacaagctc ggcgcggcgt acacgatgga gcagaacgtg ctcggcatgc
     6601 tcgacgacct ccagcaggag acgacgaacg accagctgcg gcagctgttc agccaccacg
     6661 ccgacgagac ccggcagcag atcggcaaca tcgagcaggc gttcgccgcg ctcggcgagg
     6721 aggccgacga caagccgtgc cccgcgatcc agggcctcga gaaggagagc aaggccgaga
     6781 tcaagatggc cgaggacagc gtcgtcgacg cggtgatcct gtcaggcgct gccgagaccg
     6841 agcaccacga gatcgctgtc tacgagagcc tgatcacgca ggccgaggcg atgggcaacc
     6901 agcaggtcgc ccagctcctc aagcagaacc tcgagcagga gcagcacacg ctcgaggagg
     6961 tcaagggcga gagccagcgc ctcgcgcaga cgatggcggg gtcgacggcc tgaccgtcgc
     7021 ccgctacgac tcccgcggct ccaaaatcac gagcggtatc tcgcgctccg tccgctcctg
     7081 gtagccgtgg tagccgccgt acacctcgac agccatcggc caaaggcgct cgcgctcctc
     7141 gggcctcgcg acctgcgcac ggaccggccg gcgttcccgg ccgatctgga tggtgctgtc
     7201 cgggttggcg cgcaggttgt ggaaccaggc cgggttccgc gggtagccgc ctttggaggc
     7261 gacgagcacg atgtccacgc cgtcctccac gtagacgagc gggctcaccc gcttcgcgcc
     7321 gctcttagcg cctacgtgct cgagcagtag gaacggcggc aacccgggca gcttccggcc
     7381 cacccagccc ccgctggcgc ggtacacgaa cgcgtgcccg ctgaacacgc ggttgaggat
     7441 gggccacgag ctgtcggcgg cgttcagcag gtccatgagg gcgtgattgt ggcgggccag
     7501 ggaatgatcg gtgtaggcaa gggatccgta cttcgttgtc ttcggaggga gtgggtcccc
     7561 aatgagcccc gaactgcggc acctccgtta cttcgttgcg ctcgctgagg agctgaactt
     7621 cacgcgcgcg gccgagcgcc tgcacgtggc ccagcagacg ctcagctcga ccatccagca
     7681 gctcgagcgc gagctcggag tgcagctgtt cgagcgcacc acgcgcaggg tccagctcac
     7741 cgacgcgggc aggcggctga tcgaggatgc cggcggcgcg cttggcgcgg tggagtccgc
     7801 ctggcgccac gctcgcggcg acggcgacgc cgcggccgag ctgcgcgtcg cctactccca
     7861 cacgatcggg ccgcgatgcg tgcccgcgct gcacgaggcc acgcggcggg tcctgcccga
     7921 cgtgcgcgtc acgtggtggg agatgtgggc gagcgacatc ccgcaggccg tctcctcggg
     7981 cacctacgcg atagggctcg cgcggtttcc cgtgattgat gccgacctcg agtgcgagac
     8041 ggtcgcggag gaggagcagg gcatactcgt cagctcgcgc cacccctacg ccggcttcga
     8101 caagctgcgg ctggccgagc tcgggcatga gaccttcctc gtgtttccgc gcgagatggc
     8161 gccgggctac ttcgacgcca tcgtgcgcat gctgcgcacc tttggcgggt tcgagccgag
     8221 gctgatggtg tcgcctgacg ccggcgacga cgggatcatc ccggcgatct acgaaagcgg
     8281 ctcggcactc ggactggctc cgtcgagctc cggccgtttg ttcgccgagc gctcaggggg
     8341 ccgtatcgtg ctggtcgcgc tcgaccagcc ggccccgctc gcgcccatcg acctcgtccg
     8401 ccggccggtc gctcccgacc caacagtgga gtcgctcgtc cggctggtgc gcatgctcgg
     8461 gaagcgcggg cagctcgcat gaggacgatt aactcagttc gcctgtaggt cttccagcca
     8521 ggtcctgttt gtcgcattcc ttacggttga ttagcttggc actcgccccc ctacgcgaag
     8581 ggagtgccgt gcggcaccgg ccccatgccg aatccactcc aggcgcaccg aatctgcggg
     8641 acccgagcca gttcgacgcg gcggtccgtg acctccgccc ggcggtgctt cgttgggccc
     8701 gcatcgtgct ccgcgacgac gccgccgcgg aggaccttgc gcaagaccta ttcacccagc
     8761 tctggctgaa cccggaggcc tacgactcgt cgcggacttc gttgcgctcg tatttgagag
     8821 tgctcacgcg ctcgcgcgcg atcgatcgct tccggacgcg cggagcgcac caatcggccg
     8881 cgcagcggtt ggcggtggag cagctcagcc acccgcgcgc tgcggagagc cccgaggcgc
     8941 cggcgctgcg ccgcgaggtc gcgcggcaac tgctccatgc gctcgacgag ctgccacgcg
     9001 agcagagggc cgcggtgctc ctcgcggcag tgggagagct gaccgcgagc gagcttgcac
     9061 tcgcgagcga gattccgctc ggcaccgcga agagccgcat ccggatcgga ctctcgaaga
     9121 tgcgtggcgt gctcgatgag gccgcctagc gcaggcgctt cacgagcggc gtggccgaca
     9181 ggccgtgcac gacgatcgag acgagcacga ccgccatgat cgtccacaca atctgctcga
     9241 gctcggcgcc tgcgaacgcg cctgaggcga cgatcagcgc gaggtagttg agggaggcga
     9301 tccccttcac gccgaaccag gcgagatata tccggtcgcg aaggctgagc cggtcgtcgc
     9361 ccaccagcga gacgaacacc agcacgggtc ggatcacgaa gatcagcagc ggcacgagca
     9421 gccagccgga cacgccgggg gcgccgaagt cgccccacgt gagcatcgct ccgaacaggg
     9481 cgaggaccac cagctcggcg aacgtcttca ccacctcggc cccgtcgtgc acgcggcggt
     9541 tgtagctcgt ctcgagctcc tgatggcgga agccgatccc cgcggccagc accgcgagga
     9601 agccgtagag gtcgagcgcc tgcgccgctc cgtagatgag tacggcgccc gccggcccga
     9661 gccacgggtc gagctcgggc acgagcagcc cgcggcggcg gagcgcgaag gtgcccttgc
     9721 cgaacaggta cccggcgagg cctccgagca cgatgcccgc acccgcgccg tagagcacct
     9781 cggtgccgat ccactccccc agccagctcg tgccgccctt ggtggcgacc agcgtgccga
     9841 gcaccacgaa cggcacgccg agaccgtcgt tgaagcccgc ctccgcggag agcgtgaact
     9901 cggcctcacg ctcctcctct gcggcgcccg ggggacccac cccgagcccg ccggcgagca
     9961 cggggtcggt cggcgccagc gccgcgccga gcgcgatcgc cgcgccgagc gtgagcccca
    10021 tcggccccgc ggcccaggcg gcgacagccg cgatcgtgag cgtcatcgcg atgccgagca
    10081 ggcgggccgg cgacgcccat gcgcgcgggc tgagccgccg ccgcagccgg aggcccgtcg
    10141 cgaacagggc gagcgccatc gccgcctcgc tcgcgcgccg aagcaacagc tgatcgccga
    10201 ccgggtcgag cggcccgcgg tcgaagagcc tcagccctgc gccggacagg gccccgagcc
    10261 ccaggtagac gatcgcgacg ctgtagcgcc ggtcgtgctc gtgcggcagc gcggcgaccg
    10321 cgatcacaag cgccgcgccg agtgcgatca ccgcgacgta gaaggcgctg ccgacgtcga
    10381 tgccggcgcc gccgctggcg agcgcggcgg tcagccggta ccgcccccgc gaaggaactc
    10441 gagcatgtgc tcggcgatca gctcgccctt gtcctcctgc aggaaatggg ccgcaccctc
    10501 gatcctcacc tgctcgcccg cccccggcag caggtcgcag aacacctggc cggagcgcgg
    10561 gaaggggaac accggatcgg agtccgaaaa ggcgacaagc gcgggcttct cgaaacgcga
    10621 cagcgcgtcc gcgacggcgc gcatctcggc ggcgcccgcg tcctcgtcgg aagttggaac
    10681 gatgagagga aactgcgccg cgccggcctt cgactcgggc gtcgggaacg gcgcctcgta
    10741 ggccgccaag acttcggggg gcacctcggt ggccgtcgcg cccgctacga cgaagccgac
    10801 gggcatgtcc gggttgcgct cggcgaagtc gcgccacgcc atgaacccct tcgacacgcg
    10861 gccggtgaag agacccgtgt tcaggattgc gaggccgccg acgcgcgcct cgttctcgac
    10921 ggcccagcgc agcccgatcg ggccgcccca gtcctggacg accacggtcg cgcgtcggag
    10981 atcgagcgtg tcgagcacgc gcgacacgtg atccacgtgg cggtcgtagc cgtaccagtc
    11041 gcgatcgagc ggcttgtcgg agcgcccgaa gcctgcgtaa tccggagcga tcacgcggta
    11101 gcccgcgtcc acgagcggcg ggatcatctt ccggtacagg tacgaccagg tcggctcgcc
    11161 gtgaaagcac acgatcgcct caccctcgct cgggccctcg tcgacgtaat gcatgcgcag
    11221 cccatcgacg tcgacgtagt gcggatcgaa gtcgtagccc gggaggccgc ggaagcgctc
    11281 ctccggcgtg cggtagagct cgagcgtgct catccgccag gcagcgcgaa gaacgcaacg
    11341 gcggcgtagt gcagcgccgc cgccccgatc accagcgcgt ggaacacctc gtggtagccg
    11401 aagacggccg ggaccggatc gggcctgcgc accgcgtaca cgaccgcgcc tgccgtgtag
    11461 agcagaccgc cggcgagcac cagcccgagc gccacggcgc cggcgtggtc aagtatctgc
    11521 ggcgtggcgg cgacggccac ccagccgagc gccacgtaga ccgccgccac gagccacttg
    11581 ggcgcgtcga tccagaggag cttcagcagg atcccgccga gcgcaccgcc ccacacaacc
    11641 gcgagcactg tgttcgcgat tcctccgcgc aggacgagca ggcagaaagg cgtgtacgtg
    11701 cccgcgatca ggaggaagat catcgagtgg tcgagccggc gcatccagcg gcgcgccgaa
    11761 ggacgccagg tgatgcggtg gtacagcgcg ctcgtgccga acagcgccga cagcgacacc
    11821 gcgtagatcg ccgcggcgag cgcggctttg cgccccggcg cgagcacgag cagcgcggag
    11881 ccggccacga gcgacacgaa gaacgcccac tggtgcgtca caccgcgaag gcgcggcttg
    11941 atcggggtca cggcgggcga gacctccatg cgcggaatgt acctaggaag cggcggcgat
    12001 tcgctgatcg gcccgcaccg ccccgatcgc ttcgagcaac accgccgaga actcgtcggc
    12061 gttcacgatc accgcgctgt ggtccgcggg gatctcgaag cgcacggcgc cgagcagatc
    12121 ggcgagctcg tgctggcgcc gcgccggcac cccgcggtcg cgcgacgtga cgaccacagc
    12181 cgcgggcttg tcgagcgagc gggcccatgc gcggctgtcg aagcgcccga gctcgcgccc
    12241 ggcctcggcg atgtcgaggc tcgagcctcg cgacagctcc gacgcgaccc acgtggtggc
    12301 cggcgagtcg gggaaaccgc cggcgcgcag gccggcgcgc caggcgccct gcgggaacag
    12361 gccgagtccg aggcgcagag cgcccatcga cttccacagc cgccgcatgc gtggctcgac
    12421 ccacgtggct gcggtcgcac agttcacgag ccccgacacc gtgtcgccgt ggtcgcgcgc
    12481 catgagctgc gagatcgggc cgcccatcga gtagccgact gcggtgacgg ggccgcagcc
    12541 gaggtggcgg acgagcgcgg cggcatcggc ggcgcagcct tcgaggctga acggctctgc
    12601 cggacgcagc ccgcggccgt gtccgcggtg gtcggtggcg agcaccctgt atccggcctg
    12661 cgcgagcggc tcgtacgtgc ggaaccagtt caggtcggac tggaacatcc agccatggag
    12721 cagcaggacc ggtgggccgt cgccgcccgt atcgcgcagg aacatctcgc ctgtgccgtg
    12781 cacgtgcacg atccggccct ccggcagctc gaccggaggc tcgggcatct caggccgcgt
    12841 caccggccga gctctccctg tgcctcgtca tgtcccgcga ccagcgcgat ctcgatgtaa
    12901 gagatgacgg gttcgagctc gacgagcttg tcgaactgtc cgaggcgtac gcgctcccag
    12961 gtgagctcgt gcactgcgcc gacgatcgcg ctgaacacct cgggcggcgg ctcgggaagc
    13021 tccggatgct gctccctcgc gcgcgcggtg agtgcgcgca cgcgatcggc gaagcgccgg
    13081 tgcacctccg cgcgccgctc gagcgcggcg gggccggcgg cgagcacctc gatcatgaag
    13141 gtgcgcgcga a
//
LOCUS       DASVXS010000199         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1398668, whole genome shotgun sequence.
ACCESSION   DASVXS010000199 DASVXS010000000
VERSION     DASVXS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398668"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VF752_11050"
     CDS             <1..477
                     /locus_tag="VF752_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856128.1"
                     /translation="VIGISVVLGPRRRRDRLIVRLRASGRLCASKPATDEEGIMRVTG
                     ITANLPVADIATASEFYREYLGLSEEAMHLGWVARYASADGRASVQLVAGDASAPQDS
                     VISVHVGADVDEAYAEARRRGYEIVHPLTDEPWGVRRFFVRAPDGNIINIVSHSDD"
     gene            complement(474..824)
                     /locus_tag="VF752_11055"
     CDS             complement(474..824)
                     /locus_tag="VF752_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856129.1"
                     /translation="MINGVHAIIFSDDAEGVRAFFRDVLGLPSVDAGGGWLIFALPPA
                     ELAAHPDGGESRHELYLMCDDIGSTVKEMKEKGVEFTREIRDEGFGLMTALRLPDGSE
                     LAIYEPRHPSPLSP"
     gene            988..1839
                     /locus_tag="VF752_11060"
     CDS             988..1839
                     /locus_tag="VF752_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8856130.1"
                     /translation="MAESRPTARAAEALDSESGYRLAAAGREAAEDERLTLLEQLYDP
                     VSCRRRALVQRGWRCLEVAAGRGSMAVWLAEQVGPAGHVVATDIDTRYLRRLELQNLE
                     VLEHNILEDPLEALGPGSFDLVCSRLTLFHLVGRQEQALRQMTKCLRPGGWLIDEDAD
                     WGTAAPVDPAHPLYDGYRRAWQDGDWWTSRGYDKAFGQKLPALFERCGLQDIRHEAAT
                     EVVRGGSPWARWWAVTLEVINELGGGDESDGRELEVMTTALADPTVWVLRELLHACRG
                     RRVGDTA"
     regulatory      2102..2231
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2544..2969
                     /locus_tag="VF752_11065"
     CDS             2544..2969
                     /locus_tag="VF752_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856131.1"
                     /translation="MTASRPPLHVRLSARLRRASLDRALADGTDPATSPELEWRARQL
                     ASPGTYRELAERLEEVVDEAGEPHPNAVVQIDREAVRCAREDLLTLAAQLAIADRANA
                     RGVALVRRLLTEGLSPLYVPSSPERLERFVRQARAALGT"
     gene            complement(3036..3185)
                     /locus_tag="VF752_11070"
     CDS             complement(3036..3185)
                     /locus_tag="VF752_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856132.1"
                     /translation="MAKDHGRSVKNDKQYEGLRKKGMSKERAAKIANTPNASKKGGKK
                     SGKSS"
     gene            complement(3249..>3817)
                     /locus_tag="VF752_11075"
     CDS             complement(3249..>3817)
                     /locus_tag="VF752_11075"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8856133.1"
                     /translation="VISLGGIVCSRSVRTGGYELDEAIAAYVRRKYGIAMGQQTAEDI
                     KFEIGSATQLEQESTVEVRGRDLASGLPKQIMLTSEECRDAIAEPLHTIIDAVKDTLE
                     ETPPELASDISERGILLAGGGALLQGFDERVRDETGMPASLAESPLTCVAVGSGRSLE
                     EFDAIAASDARAAKRARQRNARARASVR"
BASE COUNT          570 a         1263 c         1334 g          650 t
ORIGIN      
        1 gtcatcggga tctcggtcgt cttgggccct cgtcggcggc gcgatcgact catcgtccgg
       61 ctgcgagcct ctggcaggct ctgcgcatcc aagcctgcca ccgacgagga gggaatcatg
      121 cgggtaaccg gcataaccgc gaacctcccg gttgccgaca tagcgactgc gagcgagttc
      181 taccgggagt acttggggct gagcgaggag gcgatgcacc tcggctgggt ggccaggtac
      241 gcctccgcgg acgggcgggc atcggtacag ctcgtcgccg gcgatgccag cgcgccgcag
      301 gattccgtca tctcggttca cgtcggcgcc gatgtggacg aggcatacgc cgaggctcgc
      361 cggcgggggt acgagatcgt acatccgctc accgatgaac cttggggcgt gcggagattc
      421 ttcgtccggg cccccgatgg caacatcatc aacatcgtca gtcattcaga cgactagggc
      481 gacagcggac tcgggtgacg gggctcgtag atcgccagct cactgccgtc gggaaggcgc
      541 aatgcggtca tgaggccgaa tccttcgtct ctgatctccc tggtgaactc gacgcccttc
      601 tccttcatct ccttcacggt ggacccgatg tcgtcgcaca tcaggtagag ctcgtggcgg
      661 ctctccccgc cgtccgggtg ggcagcgagc tcggcgggcg gcagcgcgaa gatcagccag
      721 ccgccgccgg catcgacgga ggggagccct aggacgtcac gaaagaacgc gcgcaccccc
      781 tcggcgtcgt ccgagaagat gatcgcgtga accccgttga tcaaggctca accactctat
      841 ccgcggcgac cgcgaggccg acccagacat gagtctgatc ggggtctgcg ttccctgtgc
      901 gtcgcgatgc ctgccgctcg tccaaccgcg cggcccgatc gaaccgactc gcttgctcga
      961 gtcggcccgc ggggctacgt tgtcaccgtg gcggagtcga ggcccacggc tcgagctgca
     1021 gaggctctcg acagcgagtc tggctatcgc cttgctgcgg cgggccggga ggcggccgag
     1081 gacgagcggc tgactcttct cgagcagctc tacgatccgg tctcgtgccg gcggcgggca
     1141 ctggttcagc gcggctggag gtgtctggag gtcgcggcgg ggcgaggctc gatggcggtg
     1201 tggctggctg agcaggtcgg tccggcaggg cacgtggtcg cgaccgacat cgacacccgg
     1261 tatctgcgcc ggcttgagct tcagaatctt gaggtgctcg agcacaacat cctcgaggac
     1321 ccgctcgaag cgctgggccc gggttcgttc gatctggtct gctcccgttt gacgctgttt
     1381 catctcgtgg gcaggcaaga gcaggcgctc aggcaaatga cgaagtgcct gcgcccaggt
     1441 ggctggctga tcgacgagga cgccgattgg gggacagccg ctcccgtcga tcccgcgcac
     1501 ccgctgtatg acggctaccg gcgcgcctgg caagacgggg actggtggac ttctcgcggt
     1561 tacgacaagg cgtttgggca aaagctgccg gcactgtttg agcgctgcgg tcttcaggac
     1621 atacgccatg aggcggccac cgaggtggtg cgcggaggct cgccttgggc tagatggtgg
     1681 gcggtgacgc tggaggtgat caacgagctt ggcgggggcg acgagtcgga tgggcgtgag
     1741 ctcgaggtga tgaccaccgc gctcgccgat cccactgtgt gggtcctgcg ggagttgtta
     1801 cacgcctgcc ggggcaggcg ggttggcgac accgcgtagc cgcttcggga tctagcggtg
     1861 gggcgtcgga agggatgtac cagcgcgcgc cggtgcgagc cggggagcgg acgcatccag
     1921 ccgcatgggc tcccgcgcgc accgtgctca gacgcggccg ctggccgttg agccccgaga
     1981 ggcacgagag ccgcgcagag acagcccgca attcgcggaa agagatggtg ccgatcggaa
     2041 gcttcgttac cgagattgcg gtacaacggc cgtggtaatc caaatgcagc ccaggccgaa
     2101 ctgtcgtgcc gcgcccggcg gcaatggcag ccgggggaac caacaagttg gggcgaatcg
     2161 tcgctaggcg tagcgcgacc tctttccgcg cgagcccgtc agctaacccc ggaggcgcaa
     2221 cgaaagagag ggactatgac aagggccgcg cgcctgctcg aagctgtcga cgcacgccta
     2281 cggcggggcg actctttttc gagagtcgaa gaggaggtga tcgagagcag ctccctggat
     2341 gagcggcaca agtctgccct atggctgtat gcctggtcct tcgtgggtcc ccaacagcag
     2401 cgttgcgaag cgctgggcct tcttatgacc gctgtcgagc gtgatggggg caccgccgga
     2461 ggcagccggt cgagctgagg tacgcgcgaa ccagataatc cgttttccgg ttgtgggcag
     2521 cccttggcgg gcatttgacg ggcatgacgg cctcgcgccc gccactgcac gttcgcctga
     2581 gcgcccgcct gcgccgcgcc tcgctcgacc gggcgctcgc cgacggtacc gatcccgcga
     2641 ccagcccgga gctcgagtgg cgcgcgcgac agctcgcctc tcccggcacg taccgcgagc
     2701 ttgcggagcg gctcgaggag gtcgttgacg aggctggcga gccgcacccc aacgcggtcg
     2761 tgcagatcga tcgcgaggcg gtccgctgcg cacgcgagga cctgctcacg ctcgccgctc
     2821 agctcgcgat cgcggaccga gcgaacgccc gcggcgtcgc cctcgtgcgg cgattgctca
     2881 cagaaggctt gtcgcctctc tacgtcccaa gcagcccgga gcgcctcgag cgcttcgtgc
     2941 gccaggcgcg cgctgcgctg ggcacctgag cctcagcccg tgtcagggct ctgatgcgcc
     3001 gtggcctgag tgggccgggg cgctcgcgcc ccggctcagc tgctctttcc gctcttcttt
     3061 ccgcccttct tggaggcgtt gggcgtgttt gcgatcttgg ccgcgcgctc cttgctcatg
     3121 cccttcttgc gcaggccctc gtattgcttg tcgttcttga cgctgcgtcc gtggtccttg
     3181 gccatgagac ctcctggtcg cttccgcgtg gacgcctacc ccgcgtgaaa tgcctgcaaa
     3241 cgcgcgagtc agcgcacgct cgcgcgggca cgcgcgttgc gctggcgtgc gcgcttcgcc
     3301 gcgcgggcat cgctcgccgc gatcgcgtcg aactcctcga gcgacctgcc ggagccgacg
     3361 gccacgcagg tgagcggcga ctcggcgagg ctcgcgggca tgccggtctc atcgcgcact
     3421 cgctcgtcga agccctgcag cagcgcgccg ccgccggcga gcaggatgcc gcgctcggag
     3481 atgtccgacg ccagctccgg cggggtctcc tcgagcgtgt ccttcaccgc gtcgatgatc
     3541 gtgtgcagcg gctccgcaat cgcgtcgcgg cattcctcgc tcgtgagcat gatctgcttc
     3601 gggagaccgg aggcgagatc gcgcccgcgc acctcgaccg tggactcctg ctcgagctgg
     3661 gtggccgagc cgatctcgaa cttgatgtcc tcggccgtct gctgtcccat cgcgatgccg
     3721 tacttgcgcc tcacgtacgc ggcgatcgcc tcgtcgagct cgtagccgcc cgtccgcacc
     3781 gagcgcgagc agacgatgcc gccgagcgag atcaccg
//
LOCUS       DASVXS010000200        12094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1087854, whole genome shotgun sequence.
ACCESSION   DASVXS010000200 DASVXS010000000
VERSION     DASVXS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087854"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VF752_11080"
     CDS             complement(<1..1002)
                     /locus_tag="VF752_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8856134.1"
                     /translation="MGEQADVLVIGAGIAGLVTTLELLEHGRDVLLLDRCRPEELGGL
                     AREAFGGMFMVDSPEQRRSGVEDSVELALADWLRIADFDESDEWPRRWAEQYVSRARD
                     DVGGWLKRHGVKFFPVVNWAERGVYGDGNSVPRFHLTWGTGKALVDHVWAAIERHPRR
                     RSLDLRFHTRVTELLEDGGRVRGCRAESEDGGGETEIEAAVVVVAAGGVGGNLDIVRR
                     EWPAQLGDPPEHILMGSHYYADGAMHEEVKRIGGNVTHINRMWNYADAVRHPHPRRPL
                     HGLKLIPPRSGLLFDPTGRRYGPVPVMPTFDAYYALERMCEDERKYYWMICNWKIARR
                     "
     gene            complement(1043..1273)
                     /locus_tag="VF752_11085"
     CDS             complement(1043..1273)
                     /locus_tag="VF752_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856135.1"
                     /translation="MERNFMQSEVHRQHELMDRSGLGLLPGIAIAFAIVLAIMAALVI
                     QEWWVTFAVLGALFAVTGAVVWVILKLLGDED"
     gene            complement(1557..2516)
                     /locus_tag="VF752_11090"
     CDS             complement(1557..2516)
                     /locus_tag="VF752_11090"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX8856136.1"
                     /translation="MATTTLNEQITKYLTDVHSIEEQALIQMKSAPKIAGDDELARVF
                     SEHLVETEEHERRVRALLEARDSQPSKLKDVAGKAGGVGFVLFARAQPDTPGKLTAHA
                     FSYEHMELAAYDLLSLFAERADEQDVVELAQAIGPQEKRMAERLAANFDRAVEASLRE
                     VADDELENRLIDYLADAHAIEAQAIQMLQAAPKIVGESELAKVFRDHLEETRAQQASV
                     EARLKAHDAGPSRLKDAAMRVGALNWGAFFGAQPDTPAKLAGFAYAFEHLEIAAYEEL
                     KRVAARGDDTDTVRIADEIIPQERGTAALIHAEFPRAAEATLD"
     gene            2677..3903
                     /locus_tag="VF752_11095"
     CDS             2677..3903
                     /locus_tag="VF752_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX8856137.1"
                     /translation="MASTSGSLESLRERNRMRVIDALRTKGAVSRADIARETGLSRST
                     VSSLIADLQSAGLVVERDGNGGPTSGPRGGRPGVLVALDSSAGAVLGLDFGHKHLRVA
                     LADLSYSVITERFTDLEVDTAAPEALDTATALASELIDESGMTGRVLAAGMGLPGPID
                     TDSGLVHSQSILPSWVGLSPAAEMEKRLNLTVHLDNDANVGALGESTFGASRGAEVMA
                     YLRLSAGIGAGLVIKGRPFRGANGTAGEIGHVMIDPSGPICRCGNRGCLETVAAGPAL
                     CELVRRSHGQLTVAQLLALAQRGDAACQRVIADAGRAVGRAVADLCNYLNPNLVVVGG
                     ELSGAGDLVLEPIREAVRRFAIPAAADDVEIVAGTLGDRAELLGALALAGHESEDMLT
                     VPVPPITTSPQGGRGR"
     gene            3903..5018
                     /locus_tag="VF752_11100"
     CDS             3903..5018
                     /locus_tag="VF752_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX8856138.1"
                     /translation="MTSPNSRRVLAIGGALVALSVGVAACGGGNNNSSTSGGGGKADK
                     VGVLLPDTKSSVRWESFDRPLLADAFKAAGVSATINNAQGDKSTQQQQAEQMITNGAK
                     VLLLVNLDSGSGAAIEANAKSRGVKVIDYDRLTLKGSAAYYVSFDNVKVGKLQGQGLV
                     NCLNKDGAKNPNIAELNGSPTDNNATLFKQGYDSVLNPLYKSGKAKKVADQSVPDWDN
                     QKALTIFEQMLQKENNNIQGVLAANDGLGNSVISAVKARKLKQIPVTGQDATPEGVQN
                     ILAGDQCMTVYKAVKKEADAASKLAIALAKGTQPPAGLVNGKVNDTARDVPSVLLTPQ
                     AITKSNVKVLFDDGFLKTSDVCVGKYKSMCADAGITS"
     gene            5093..5875
                     /locus_tag="VF752_11105"
     CDS             5093..5875
                     /locus_tag="VF752_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8856139.1"
                     /translation="MNDTKPTLELRGVSKSFGAVQALYNVDFSVRAGEVMALVGDNGA
                     GKSTLIKSVAGIHPFDSGEVLFEGQPVHIHGPKDSAKLGIEVVYQDLALADNLDVVQN
                     MFLGREETTGILRSLDEVDMEKKADETLASLSVTTIRSTRQTVAGLSGGQRQSVAVAK
                     AVMWNSKVVILDEPTAALGVAQTRQVLDLVKRLGEQGLAVVLVSHNLYDVFEVADKIT
                     VLRLGQNVAVYNRKDVTQTQVVEAITAGKLSKVPGPEAEVVA"
     gene            5872..7125
                     /locus_tag="VF752_11110"
     CDS             5872..7125
                     /locus_tag="VF752_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856140.1"
                     /translation="MTTATPPPIETAPGAPAVAAPGGESLRDYVQRWWAGIRQGDLGS
                     LTIVIGLIIIAIVFQSQNSNFLTAGNFVNLIVQAAAITCIAMGIVFVLLLGEIDLSVG
                     YVSGVAGVIVALLTFPDGNQLATFPTLLLALGAGVAIGLLQGVIIAKIGVPSFVVTLA
                     GLLGWNGVVLLLIGDRGTVIIQNSFFEGFANNFVPEATAWIVAIAGVAVYAGGLLWER
                     YQRTKAGLGHAPDVLILLRVGALAAALLIVVYVAYQDRGIPYVFLVLAGVYIVLTYVL
                     NRTTFGRHVYAVGGNAEAARRAGINVDAVKIACFGICSGLAALGGIILASRLRSVDTN
                     SGGGSILLYSIAAAVIGGTSLFGGRGSVKSAVLGALVIASIDNGLGLLGLSSGTKFVV
                     TGLVLLAAVVVDSLARRGREQAGRA"
     gene            7203..7910
                     /locus_tag="VF752_11115"
     CDS             7203..7910
                     /locus_tag="VF752_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8856141.1"
                     /translation="MEGLRGKSALVTGASGGIGAAVSRQLHDAGARVAMLSRRGDDLG
                     LDGEIGIACDVRDRADVRRATEDAVARLGQLDIVVANAGVGSYGPFTEMDLDQVDSMI
                     DTNLKGIIWTAAATIPHLVEAGGGDFVSIASIAGLNAFPGEAVYNASKFGQVGFVRAL
                     DIELREKGVRCTTIAPGGVNTHFAIGTGRTEGDPELEEMMTADEVAEVVLFALTRPRN
                     MRLMTTTFRPMSEGSWG"
     gene            7907..8875
                     /locus_tag="VF752_11120"
     CDS             7907..8875
                     /locus_tag="VF752_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8856142.1"
                     /translation="MSLRWGVLSTANITDKLLNSGHDQEFTAVASRNTSRAREWANAR
                     GIPTAYGSYEELLADPDIDAVYIPLPNSLHVPWSIAALEAGKHVLCEKPMSRHPKDVE
                     RAFDVAEREGRVLMEAFMWRHHPQLQRTRELLAAGEIGPLRVIRAAFAFLADDPDDIR
                     LNPELDGGGLMDVGCYCVSGCRALTGAEPEIAYAEQVIGGKGVDVVMSATLRFPGDVL
                     AHFDCGLAYEGGDQLEAVGDDGSIFLDDPWHGREAEIEVRRPDGSIERIEAGPANSYA
                     YELANFEAAVRGEQPPLLGRDDALAQARAIAALYSSAERKAPCPID"
     gene            8860..9879
                     /locus_tag="VF752_11125"
     CDS             8860..9879
                     /locus_tag="VF752_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX8856143.1"
                     /translation="MPDRLKTSLGIWALGGMVTRFVPVGYQPDHGAESTVQKVRRAVE
                     GLGDLMDDYEFHYPNELSRDNLDDVRDALDGHGIYAVAGGLHLDPRFGKGGLSSPDDR
                     LREDALRMTLDAAEFAGEIGAEMIIWPGIEGYNYPFQTPYAESWARMIDGIGQAAEVL
                     ARHGRKLFLEHKNSEPAMKIFMRNIGMTLHVIHTLQRRGIDNVKVNMDWQHLIMNGEH
                     LPEYAALLAAEGLLGHQHANSGWGTFDDDNMVGATAFMETLELALELRRAGYGEHGER
                     LGFDLYPYTEDQVAAVRRSVLHWQFIDDVAQRVDGDGLREAQQQKDAVRAYELVYEAL
                     GARAA"
     gene            9876..11303
                     /gene="xylB"
                     /locus_tag="VF752_11130"
     CDS             9876..11303
                     /gene="xylB"
                     /locus_tag="VF752_11130"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX8856144.1"
                     /translation="MTRLVGLDVGTSSVKGVAIDAGGKVLAVAEQPHDLSMPRPGWSE
                     QDPDDWWRGAAVVLEQLDSASADGIGLTGQMHGLVTLDQAGHPLRPAILWNDGRTEAE
                     CAEIEKKVGFERLVELTGNRAQAGFTAPKILWLRNNEPEVYARIAHILLPKDYVRLRL
                     TDLHAIDVADASGTLLLNVAERRWSDEMLDALEIDRAWLPNVFESPEQTGTTHGGVPV
                     AAGAGDQAAGALGVGVVAEGGPASVVLGTSGVVFAALDRYAHDPQARVHAFCHAVPNA
                     WHVMGVMLSAAGSLGWLRSAIGGTYDELLAEAADWEPGVEGLLFAPYLAGERTPHADP
                     SARGAFAGLSLRHNRGAMARAVLEGVAFGLRDALDLVEQLGGRPRVGRVSGGGARSQL
                     WLEIVASVLDLPLELTVVDEGAAYGAALLGGVASGVFADAREAVAECVKVTRVVEPRA
                     DWVEAYAEIRERFRGLYPALRPLIG"
     gene            complement(11273..>12094)
                     /locus_tag="VF752_11135"
     CDS             complement(11273..>12094)
                     /locus_tag="VF752_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8856145.1"
                     /translation="AAGVTWDQVAHTVVGGPGVFDPDSDRLRHAPNLPGWSRPGLMSS
                     LREALPPSVALDNDANLAAVGERSYGRGREADTFVYVFVGTGIGMGIVIDGQLYRGAH
                     GAAGEVGYLPVSGDEDADPKSDTRARLRGILEEAASADAVVRTAKRLGMTGAVSAKHV
                     FADARKGDPLAVAAVEVEAERLALVVGTVAAILDPELVLLGGGVGGNLDLLRPPLERK
                     LAELTPLGTRIEEGKLGQDAIVLGAIASALDTARDRVFEQRAGMAAQPMSGRSAG"
BASE COUNT         1889 a         4160 c         4192 g         1853 t
ORIGIN      
        1 gcgccgcgcg atcttccagt tgcagatcat ccagtagtac ttgcgctcgt cctcgcacat
       61 gcgctcgagc gcgtagtagg catcgaacgt cggcatcaca ggaacgggtc cgtatcgccg
      121 cccggtcggg tcgaagagca gccccgagcg cggcgggatc agcttgagcc cgtggagcgg
      181 ccgccgcggg tgcgggtggc ggacggcgtc ggcgtagttc cacatgcggt tgatgtgggt
      241 cacgttgccg ccgatccgct tcacctcctc gtgcatcgcg ccgtcggcgt agtagtgcga
      301 gcccatcagg atgtgctcgg gcggatcgcc cagctgggcg ggccactcgc gccgcacgat
      361 gtcgagattg cctcccacgc cgcccgcagc cacgacgaca accgcggcct cgatctcggt
      421 ctctccgccg ccgtcctcgc tctccgcgcg gcagccgcgc acgcgtccgc cgtcctcgag
      481 cagctcagtc acgcgcgtgt ggaagcgcag gtcgagcgag cgcctgcgcg gatggcgctc
      541 gatcgcggcc catacgtggt ccaccagagc cttgccggtg ccccacgtga ggtggaagcg
      601 cggcacggag ttgccgtcgc cgtagacgcc gcgctcggcc cagttgacca ccggaaagaa
      661 cttcacgccg tgccgcttaa gccagccgcc aacgtcgtcc cgcgcgcgcg agacgtactg
      721 ctcggcccag cggcgcggcc actcgtccga ttcgtcgaag tcggcgatcc gcagccaatc
      781 ggcgagagcg agctcgacgc tgtcctccac tccggaacgg cgctgctcgg ggctgtcgac
      841 catgaacatc ccgccgaacg cttcgcgcgc gaggccgccg agctcctcag gccgacagcg
      901 gtcgagcagg agcacgtcgc gtccgtgctc gagaagctcc agagtcgtga cgagcccggc
      961 aatgcccgcg ccgatcacaa ggacgtcagc ctgttcgccc attcgcggcc ggaagcctaa
     1021 gccccggccg cggcgcgtgt cgctagtcct cgtcgccgag cagcttcagg atcacccaca
     1081 ccaccgcgcc ggtcacggcg aacagcgcgc cgagcacggc gaatgtgacc caccactcct
     1141 ggatgacgag ggcggccatg atcgcgagga cgatggcgaa ggcgattgcg atcccgggta
     1201 gaagccccag cccggagcgg tccatcagtt cgtgctgacg gtggacctcg ctctgcatga
     1261 agttccgttc catgtgagcg atgatcggct cggacgacgc cacggtcatc cgggaccagc
     1321 cgcctaccgg ctgagtgctt tctactgatc gcgggtggcg accgcgatca gcgcgtccac
     1381 cacctgcgga tcgaactgcg tgcctgaatt gcggcgcagc tcctggatcg cgtcctcgag
     1441 cggcatggcg gcgcgataag acccgcgcat cacgccgcca actcgatcga gaagcacctg
     1501 ccaaggaatt ggccggcgga ggcggggttc ggcccgcccc cgcccgctcc tttgcgtcag
     1561 tcgagcgtgg cttccgccgc gcgcgggaac tcggcgtgaa tgagggccgc cgtgccgcgc
     1621 tcctgcggga tgatctcgtc cgcgatgcgc accgtgtcgg tgtcgtcccc ccgggcggcg
     1681 acgcgcttca gctcctcgta ggccgcgatc tcgaggtgct cgaaggcata ggcgaacccg
     1741 gccagcttcg ccggcgtgtc gggctgcgcg ccgaagaacg cgccccagtt gagcgcgccc
     1801 acgcgcatcg ccgcgtcctt gagcctcgag ggaccggcgt cgtgcgcctt gagccgcgcc
     1861 tcgacgctcg cctgctgggc acgcgtctcc tccagatggt cgcggaagac cttggcgagc
     1921 tcggactcgc ccacgatctt cggcgcggcc tggagcatct ggatcgcctg cgcctcgatc
     1981 gcgtgcgcgt cggcgaggta gtcgatcagc cggttctcga gctcgtcgtc cgccacctcg
     2041 cgaagcgagg cctcgaccgc acggtcgaag ttcgcggcca gccgctccgc catccgcttc
     2101 tcctgcggcc cgatcgcctg agccagctcc accacgtcct gctcgtccgc gcgctcggcg
     2161 aagagcgaca gcaggtcgta cgcggccaac tccatgtgct cgtacgagaa cgcgtgcgcg
     2221 gtgagcttgc cgggagtgtc cggctgagcg cgcgcgaaca gtacaaagcc gacgccgccc
     2281 gccttgccgg ccacgtcctt cagcttgctc ggctgcgagt cgcgggcctc gagcagcgcg
     2341 cggacgcgcc gctcgtgctc ctcggtctcc accaggtgct cgctgaacac gcgcgcgagc
     2401 tcgtcgtcgc ccgcgatctt cggcgcgctc ttcatctgaa tgagcgcctg ctcctcgatc
     2461 gaatggacgt cggtgaggta tttcgtgatc tgctcgttga gggtcgttgt tgccatgaca
     2521 ggggcctacc cagcgctgcg gggctgactc ctcgggcgtc cttcgcaccc aaaaccgttg
     2581 ggttcgtgcg ggcttcgtat taagtcaggg ttaattcgct tccggttcgt ccgaagcctt
     2641 gacaaaccgg ccgccgctct gagagggtcg ccaatcgtgg cctcgaccag cgggtcgttg
     2701 gagtcgcttc gagagcgcaa ccgtatgcgc gtcatcgatg cattgcgcac caagggcgcc
     2761 gtcagtcgcg ccgacatcgc gcgggagacc gggctcagcc gctccaccgt gtcgagcctg
     2821 atcgccgatc tgcaatccgc cggcctggtc gttgagcgcg acggcaacgg cggcccaacg
     2881 agcggtccgc gtggcggacg gccgggcgtg ctcgtcgccc tcgacagctc cgccggcgcg
     2941 gtcctcggac tcgacttcgg ccacaagcac ctgcgcgtgg cgctcgccga cctgtcctac
     3001 tcggtgatca ccgagcggtt cacggacctc gaggtcgaca ccgcggcgcc cgaggcgctc
     3061 gacaccgcga cggcactcgc ctcggagctg atcgacgagt cggggatgac cggccgcgtg
     3121 cttgccgccg gcatgggcct cccgggcccg atcgacaccg acagcggcct cgtccactcc
     3181 cagtccatcc tgccgagctg ggtggggctg agcccagcgg ccgaaatgga gaagcggctg
     3241 aacctcaccg tgcacctgga caacgacgcc aacgttggcg cgctcggtga gtcgaccttc
     3301 ggtgccagcc gcggcgctga ggtgatggcc tatctgcgac tttcggccgg catcggcgcc
     3361 ggccttgtga tcaaagggcg tcccttccgc ggcgccaacg ggacggctgg ggagatcggg
     3421 cacgtgatga tcgatccctc tggcccaatc tgccgctgcg gcaaccgcgg ctgcctcgag
     3481 acggtcgctg ccgggcccgc tctatgcgag ctcgtgcggc gatcgcacgg acagctcacg
     3541 gtcgcgcagc tcctcgcgct cgctcagcgc ggtgacgcgg catgccagcg ggtcatcgcc
     3601 gatgccgggc gcgccgtagg tcgggcagta gccgacctgt gcaactacct caatccaaac
     3661 ctcgtcgtgg tgggcggcga gctgtcgggc gcgggtgacc tcgtcctcga gccgattcgc
     3721 gaagcggttc gccgctttgc gattccggct gccgcggacg atgtcgagat cgtggccggc
     3781 acgctcggcg accgtgccga attactcggt gcgctcgcgc tcgcagggca cgaatccgag
     3841 gacatgctga cggttccagt accccccatt acaactagtc cacaaggagg gagagggcgg
     3901 tgatgacatc accgaattcc cgccgcgtgc tcgcaatagg cggcgcgctg gtggctctgt
     3961 cggtcggcgt cgcggcatgc ggcggcggca acaacaactc gtccacgagc ggcggtggcg
     4021 gcaaggccga caaggtcggc gtgctgcttc cggacaccaa gtcgtcagtg cgctgggagt
     4081 cgttcgaccg gccgcttctg gccgacgcct tcaaggctgc gggcgtctcg gccacaatca
     4141 acaacgccca gggcgacaag tcgacgcagc agcagcaggc ggagcagatg atcaccaacg
     4201 gggcaaaggt gctcctgctg gtgaacctcg actccggctc gggtgcagcg atcgaggcca
     4261 acgccaagtc ccgtggcgtg aaggtgatcg actacgaccg cctcacgctc aagggctcgg
     4321 ccgcgtacta cgtgtccttc gacaacgtca aggtgggcaa gctccagggc cagggcctcg
     4381 tgaactgcct caacaaggac ggcgcgaaga acccgaatat cgcggagctg aacggctcgc
     4441 cgacggacaa caacgcgacg ctgttcaagc agggctacga ctcggtcttg aacccgctct
     4501 acaagtcggg caaggccaag aaggtcgcgg atcagtcggt gccggactgg gacaaccaga
     4561 aggcgctgac catcttcgag cagatgctgc agaaggagaa caacaacatc cagggcgttc
     4621 tcgcggccaa cgacggcctc ggcaactcgg tcatctctgc ggtcaaggcc cgcaagctga
     4681 agcagatccc ggtgaccggt caggacgcca cgcctgaggg tgtgcagaac atcctcgccg
     4741 gcgaccagtg catgacggtc tacaaggccg tgaagaagga ggcagacgcc gcctccaagc
     4801 tggcgatcgc gctggccaag ggcacgcagc ccccggccgg gctcgtgaac ggcaaggtga
     4861 acgacaccgc gcgtgacgtt ccgtccgtgc tgctcacgcc gcaggcgatc acgaagagca
     4921 acgtgaaggt gctcttcgac gatggattcc tgaagacgag cgacgtgtgc gttggtaagt
     4981 acaagtcgat gtgcgcggac gcagggatca ccagctagac gaagtcacgg aaggggcgcc
     5041 gccggcggcg gcgcccctct ccgatgacag ggaccctggg agggttgaga gcgtgaacga
     5101 cacaaaacca acactcgagc ttcgcggcgt gtcgaagagc ttcggcgccg tgcaggctct
     5161 gtacaacgtg gacttcagcg tccgcgccgg cgaggtgatg gctctcgtcg gggacaacgg
     5221 cgccggcaag tcgacgctga tcaagtctgt cgccggcatc cacccgttcg actccggaga
     5281 ggtgctcttc gagggccagc cggtgcacat ccacggaccg aaggactcgg cgaaactcgg
     5341 catcgaggtc gtgtaccagg acctcgcgct ggccgacaac ctcgacgtgg tgcagaacat
     5401 gttccttggc cgcgaggaga ccaccggaat actccgctcg ctggacgagg tggacatgga
     5461 gaagaaggcc gacgagacgc tcgcgtcact gtcggtgacc accatccggt cgacgcgtca
     5521 gacggttgcg ggcctctcgg gcggtcagcg ccagtcggtt gcggtcgcga aggccgtgat
     5581 gtggaactcg aaggtggtca tcctcgacga gcccactgcc gcgctcggcg tcgcccagac
     5641 gcggcaggtg ctcgacctcg tgaagcgcct cggcgagcag ggcctcgccg tcgtgctcgt
     5701 ctcacacaat ctgtatgacg tgtttgaggt cgcggacaag atcaccgtgc tacgcctcgg
     5761 ccagaacgtg gcggtgtaca accgcaagga cgtgacgcag acgcaggtcg tcgaggcgat
     5821 cacggcgggc aagctgtcca aggtgcccgg tcccgaggcg gaggtggtcg catgacgacg
     5881 gcaacgcctc ctcccatcga gaccgctccg ggcgcacccg ccgtagccgc tcccggcggc
     5941 gagagccttc gcgattacgt acagcgctgg tgggccggga tccgccaggg cgacctcggc
     6001 tcgctgacga tcgtcatcgg gctgatcatc atcgcgatcg tcttccagtc gcagaattcg
     6061 aacttcctca ccgcggggaa cttcgtgaac ctgatcgtcc aggcagcggc gatcacgtgc
     6121 atcgcgatgg gcatcgtgtt cgtgctgctg ctgggcgaga tcgacctgtc ggtcggctac
     6181 gtctcgggcg tcgccggcgt gatcgtcgcg ctgctcacgt tcccggacgg gaaccagctc
     6241 gcgaccttcc ccacgctgct gctcgctctg ggcgcaggcg ttgcgatcgg tctgctccag
     6301 ggcgtgatca tcgccaagat cggcgtaccg tcgttcgtgg tcaccctcgc gggcctgctc
     6361 ggatggaacg gcgtggtgct cctcctcatc ggggatcgcg gcaccgtgat catccagaac
     6421 agcttcttcg agggcttcgc gaacaacttc gtgccggagg cgacggcctg gatcgttgcc
     6481 atcgcgggcg tggcggtgta tgccggcgga ctgctgtggg agcgctacca gcgcacgaag
     6541 gccgggctcg gccacgcgcc ggatgtgctc atcctgctgc gcgtcggcgc gctcgcagca
     6601 gcgctcctca tcgtggtgta cgtggcgtac caggaccggg gcatcccgta cgtgttcctg
     6661 gtgctcgcgg gcgtgtacat cgtgctcacg tacgtcctca accggacgac attcggacgg
     6721 cacgtgtacg ccgtcggcgg caacgccgag gccgcgcggc gagccggcat caacgtggac
     6781 gccgtgaaga tcgcgtgttt cgggatctgc tcgggcctcg cggcgctcgg cggcatcatc
     6841 ctcgcctcgc gcctccgctc ggtcgacacc aactcgggtg gcgggtcgat cctcctctac
     6901 tcgatcgcgg cggccgtgat cggcggcacg tcgctcttcg gcggccgagg cagcgtcaag
     6961 tcggcggtgc tcggcgcgct ggtgatcgcg tcgatcgaca acggcctcgg gctcctcgga
     7021 ctgagctcgg gcacgaagtt cgtggtcacc ggcctggtgc tgctggccgc ggtggtcgtc
     7081 gactcgctcg cacgccgagg gcgggagcaa gccggtagag cgtgacggcc ggtgggccct
     7141 cgcaccccgc tctcgtgcgg gggcccaccg cctcacgaat acgaacagaa aggcactgca
     7201 tcatggaggg actgcgcggg aagtccgcgc tcgtgactgg cgcgagcggc gggatcggcg
     7261 cggctgtctc gaggcagcta cacgacgccg gggctcgcgt cgcgatgctc tcccgccgcg
     7321 gcgacgacct cgggctcgac ggtgagatcg ggatcgcctg cgacgtgcgc gaccgcgccg
     7381 atgtgcgccg cgctacggag gacgcggtgg ctcggctcgg ccagcttgac atcgttgtcg
     7441 cgaacgccgg ggtgggctcg tacgggccgt tcaccgagat ggacctcgac caggtcgact
     7501 cgatgatcga caccaacctg aagggcatta tctggacggc tgccgccacg atcccgcacc
     7561 tggtggaggc cggcggcggc gacttcgtgt cgatcgccag catcgccggg ctgaacgcgt
     7621 tccccggcga ggcggtctac aacgcgtcga agttcgggca ggtcggtttt gtacgggcgc
     7681 tcgacatcga gctgcgcgag aagggcgtgc gctgcaccac gatcgctccg ggcggcgtga
     7741 acacgcactt cgcgatcggc accggacgga cggagggcga ccccgagctc gaggagatga
     7801 tgaccgccga cgaggtggcg gaggtcgttc tgttcgcgct cacgcgcccg cgcaacatgc
     7861 ggctgatgac caccactttc cgcccgatga gcgagggcag ctggggatga gccttcgctg
     7921 gggagtcctg tccaccgcca acatcaccga caagctgctg aactcgggcc acgaccagga
     7981 gttcaccgct gtcgcgtcgc gcaacacctc gcgcgcgcgt gaatgggcga acgcgcgcgg
     8041 catcccgacc gcctacgggt cgtacgagga gctgctcgcc gacccggaca tcgacgcggt
     8101 ctacatcccg ctgcccaaca gcctgcacgt gccgtggtcg atcgcggcgc tcgaggcggg
     8161 caagcacgtg ctctgcgaga agccgatgag ccgccacccc aaggacgtcg agcgagcctt
     8221 cgacgtcgcc gagcgcgagg ggcgcgtgct gatggaggcg ttcatgtggc gacaccatcc
     8281 gcagctccag cgcacgcgcg agctcttggc cgcaggcgag atcggcccgc tgcgcgtgat
     8341 ccgagcggcg ttcgccttcc tcgcggacga ccccgacgac atcaggctga accccgagct
     8401 cgacggcggc gggctcatgg acgtgggctg ctactgcgtg agcggctgcc gcgcgctcac
     8461 cggcgccgag ccggagatcg cttacgcgga gcaggtgatc ggcggcaagg gcgttgacgt
     8521 ggtgatgtcg gccaccctgc gcttccctgg cgacgtgctc gcccacttcg actgtggcct
     8581 cgcgtacgag ggcggcgacc aactcgaggc cgtcggcgac gacggctcga tctttctcga
     8641 cgacccctgg cacgggcgcg aggcggagat cgaggtacgc cggccggacg gatcgatcga
     8701 gcggatcgag gccgggcctg cgaactcata cgcctacgag ctcgcgaact tcgaggcggc
     8761 cgttcgcggg gagcagccgc cgctcctcgg ccgcgacgac gcgctcgccc aggcgcgcgc
     8821 gatcgcggcg ctctactcat ctgcggaaag gaaggctcca tgcccgatcg actgaagaca
     8881 tcgctcggta tctgggccct cggtgggatg gtgacgcgct tcgtgccggt cggctaccag
     8941 cccgatcacg gtgccgagtc gacggtgcag aaggtgcgcc gcgcggtgga aggactcggc
     9001 gacctgatgg acgactacga gttccactac ccgaacgagc tgagccgcga caacctcgac
     9061 gatgtgcgcg acgcgctcga cgggcacggg atctacgccg tcgcgggcgg cctgcacctc
     9121 gacccgcggt tcggcaaagg cgggctctcg tcacccgacg acagactccg tgaggacgcg
     9181 ctccgcatga cgctcgacgc cgcggagttc gccggcgaga tcggcgccga gatgatcatc
     9241 tggcccggga tcgagggcta caactacccg ttccagactc cctacgccga gtcgtgggcg
     9301 cgcatgatcg acggcatcgg gcaggccgcc gaggtgctgg ctcggcacgg gcgcaagctc
     9361 ttcctcgagc acaagaactc cgagccggcc atgaagatct tcatgcgcaa catcggcatg
     9421 accctgcacg tgatccacac gctccagcgc aggggaatcg acaacgtgaa ggtgaacatg
     9481 gactggcagc acctgatcat gaacggcgag cacctgcccg agtacgccgc gctgcttgcc
     9541 gccgagggcc tgctcggcca ccagcacgcg aactccggtt ggggcacgtt cgacgacgac
     9601 aacatggtcg gcgcgaccgc cttcatggag acgctcgagc tcgcactcga gctgcggcgc
     9661 gcgggctacg gcgagcacgg cgagcggctc ggcttcgacc tctacccgta cacagaggac
     9721 caggtcgccg ccgtgaggcg ctcggtgctc cactggcagt tcatcgacga cgtggcgcag
     9781 cgggtcgacg gcgatggact ccgcgaggcg cagcaacaga aggacgccgt acgcgcgtac
     9841 gagctcgtgt acgaggcgct gggcgcccgc gccgcatgac ccgactggtc ggtctcgatg
     9901 taggcacctc gtcggtgaag ggcgtcgcga tcgacgcggg gggcaaagtg ctcgcggtcg
     9961 ccgagcagcc ccacgatctc tcgatgccgc ggcccggctg gagcgagcag gatccagacg
    10021 actggtggcg cggcgcggcg gtggtgctcg agcagctcga ctcggcgagc gccgatggca
    10081 tcggcctcac ggggcagatg catgggctcg tgacgctcga ccaggccggc caccctctgc
    10141 gcccggcgat cctctggaac gacgggcgca ccgaggcgga gtgcgcggag atcgaaaaga
    10201 aggtcggctt cgagcggctg gtggagctca caggcaaccg ggcccaggcc ggcttcaccg
    10261 cgccgaagat cctgtggctg cgaaacaacg agcccgaggt ctacgcgcgg atcgcccaca
    10321 tcctgctgcc gaaggactac gtgcgcctga gactcaccga cctccacgcg atcgacgttg
    10381 ccgacgcgtc ggggacgctg ctcctgaacg tggcggagcg gcgctggagc gacgagatgc
    10441 tcgacgcgct cgagatcgac cgcgcctggc tgcccaacgt gttcgaatcg ccggagcaga
    10501 cgggcacgac acacggcggc gtaccggtgg ccgccggcgc gggcgaccaa gctgcgggag
    10561 cgctgggcgt aggagtggtg gcggagggcg gcccggcctc ggtcgtgctt ggcacgagcg
    10621 gcgtggtgtt cgcggcgctc gaccgctacg cgcacgaccc gcaggcgcgc gttcacgcgt
    10681 tctgccacgc cgtgccgaac gcgtggcacg tgatgggcgt gatgctcagc gcggccggct
    10741 cgctgggctg gctgcgatcc gcgatcggcg gcacctacga cgagctgctc gccgaggccg
    10801 cggactggga gccgggcgtg gagggcctcc tcttcgcgcc ctatctcgcg ggagaacgca
    10861 ccccgcacgc ggacccgtcc gcccgaggtg cgttcgcggg cctgagcctg cggcacaacc
    10921 gcggagcgat ggcgcgagcc gtgctcgagg gagtggcctt cgggctgcgc gacgcgctcg
    10981 atctggtgga gcagctcggc ggtcgcccgc gcgtgggccg cgtctcgggc ggcggcgccc
    11041 gctcgcagct gtggctcgag atcgtcgctt cggtgctcga cctgccactc gagctgacgg
    11101 tggtggacga gggcgccgcc tacggcgcgg cgctgctcgg tggcgtggcg tcgggggtct
    11161 tcgcggacgc gcgcgaggcc gtcgcggagt gcgtgaaggt cacgcgggtg gtcgagccgc
    11221 gggccgattg ggtcgaggcc tacgccgaga ttcgcgaacg gttccgcggg ctctatcccg
    11281 cgctgcgccc gctcatcggc tgagccgcca ttccggcgcg ctgctcgaac acgcggtctc
    11341 tcgcggtgtc gagagcgctc gctatcgcgc ccagcacgat cgcgtcctgc ccgagcttcc
    11401 cctcctcgat ccgcgtgccg aggggcgtca gctcggcgag cttgcgctcg agcggcggcc
    11461 gcaacaggtc gaggttgccg ccgacgccgc cgccgagcag cacgagctcg ggatcgagaa
    11521 tggccgccac ggtcccgacc acgagcgcga ggcgctcggc ctcgacctcc acggccgcga
    11581 ccgccagcgg gtctcccttg cgcgcgtccg cgaacacgtg cttcgccgat accgcccctg
    11641 tcatccccag ccgcttggcc gtacgcacga ccgcgtcagc ggacgcggcc tcctcgagga
    11701 tcccgcgcag tcgcgcgcgc gtatccgact tcgggtcagc gtcctcatcg ccgctcaccg
    11761 gcaggtagcc cacctcgccc gccgctccat gcgcgccgcg gtagagctgc ccgtcgatca
    11821 cgatccccat cccgatgcct gtgcccacga acacgtagac gaacgtgtcc gcctccctgc
    11881 ccctgccata ggagcgctcg cccaccgcgg cgaggttcgc gtcgttgtcg agcgcgacgc
    11941 tcggcggcaa agcctcgcga agcgagctca tgaggccggg gcgactccag ccggggaggt
    12001 tcggcgcgtg gcgcaggcgg tcgctgtcgg ggtcgaatac gcccggcccg cccacgacgg
    12061 tgtgcgcgac ctggtcccag gtgactcccg ccgc
//
LOCUS       DASVXS010000201        23942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_239381, whole genome shotgun sequence.
ACCESSION   DASVXS010000201 DASVXS010000000
VERSION     DASVXS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 23942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23942
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239381"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="VF752_11140"
     CDS             complement(<1..1645)
                     /locus_tag="VF752_11140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8856146.1"
                     /translation="MRSHGWRALIEQLPLAVYIDRLDEWSSNVYTSPQLEAILGYTTE
                     EWASDDRHLLKILHPDDRERVMEAHNRSRETGEPFREEYRLIARDGRVVWFLDQATVL
                     PGDAGMPALHQGFYLDITERKELEEALAARTEELGRQKRYFESLLEISPVAIVTTDLE
                     DVVTSWNPAAERLFGYTPAEAHGRKIDDLVAKSPELRAEATSVTRDAYTEGRVQAFTK
                     RTHKDGSLIDVELLAAPVLLRGEPVGTYAIYHDVRKRKRAEEALRESEQRFRVMFEEA
                     PIGVAWGPLDVSTMLVPTFLRRPGGEGEFRWNRAYREMLGYSEDELQTLHPSEYTHPE
                     DRPRQLALYRDLVAGKADRYELEMRYIGRDGRTIWAQVVDSIVRDDQGEALFGLTMVE
                     DITQRKLAEEALRQSESEVRRQKQYFESLVELSPTAIVTVDLDGNVTSWNPAAEKLFG
                     YDRQEAIGRDVDDLVARSDAVRAEAIDAAYSARKGEDLHITTQRTRKDGSLVDVDVRG
                     APIFVGEQHVGYYALYHDISDLLQARRDAEAAAEAKSAFL"
     gene            complement(1782..2165)
                     /locus_tag="VF752_11145"
     CDS             complement(1782..2165)
                     /locus_tag="VF752_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8856147.1"
                     /translation="MKQGEQIPELRVTPDKYLPHRYAGASGDFNPIHIDPEFAQAVGL
                     PSNILHGLYGMAQVARAQTQAAGGDPRALKRLSVQFRGMALPEKEIVVTGTVREERDG
                     VLVIDTVAEQEGNQIIRNAEAEIRA"
     gene            complement(2167..2607)
                     /locus_tag="VF752_11150"
     CDS             complement(2167..2607)
                     /locus_tag="VF752_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8856148.1"
                     /translation="MAVDTSIIGKSYPPHQYEVGREKIREYAYAVGESDPVHHDPAAA
                     REKGFRNVVAPPMFAVVYSAGSVGPAVLDPEVGINFAMMLHGSQEFVWGEPVVASDTI
                     TTTTTVKDIFEKDDKTFYVFESVSENQDGQEVVRGTWTNIVRGG"
     gene            2659..3693
                     /locus_tag="VF752_11155"
     CDS             2659..3693
                     /locus_tag="VF752_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8856149.1"
                     /translation="MRRLLVIGAALLAASPAAHAARLERVAHFRQPVYLTSSPSDRRD
                     VFVVERAGRVLAIRNGRVLRRPVLDIRRRVLIADRRIYKDQGGLLSVALAPDYDRTHR
                     LYAFFTARDKTIRVEEFPTGRTLVAVARTSPFDAGGQLQFGPDGALYIGMGYGGGNSR
                     VDGSALDPASPRGKILRWDPASPTAPPVPYLLGLRTPWRFSFDRGNLIVGDVGESRRE
                     EVDFLPRGRQDGANLGWPAFEGTLRHGPFAAPGAIPPVLQKRHSSGWCALIAGYVVRD
                     RLLHDLYGRYVYGDLCKSGIRSVRLSARGARADRSERLAVPNLVSFGEDARGHLYAVS
                     LDGWVYRFAG"
     gene            complement(3675..4553)
                     /locus_tag="VF752_11160"
     CDS             complement(3675..4553)
                     /locus_tag="VF752_11160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8856150.1"
                     /translation="MPEPPQTVAVLGTGIMGAPMARHIAGAGMRVRAWNRSREKADAI
                     AGDGIDIAGLPAEAVESADVVVTMLADGDAVLSVMKEAIPAMRDDAVWDQMSTIGISA
                     TERVAALADEHRVTLVDAPALGTKEPAEKGELIVLASGPDEALDVCAPIFEAVGSRTI
                     RVGDAGAGTRLKLVINNWLLGLVGTLAETIAFAQEIDVDPRNFLDAIDGGPLGPAYAR
                     LKGEMMIDDRFDPSFPARLALKDAELVLEAAERHGLVLPIAETVARQFEKAISEGHAD
                     DDMAAVYDAWRQPANR"
     gene            complement(4597..5793)
                     /locus_tag="VF752_11165"
     CDS             complement(4597..5793)
                     /locus_tag="VF752_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX8856151.1"
                     /translation="MTEDWVGRLADLAVRFGANVQEGQVVAVSTEPGKEPLARAVADA
                     AYRAGAKFVDVATYDAYVKRARLLHGSEDAIGFVPSWLGERVLELGRQHGASVGLTGA
                     VAPDLLEDVDPERVGKDTWPALEEWAVVVSERSVNWTAVPCPTPDWAALVYPDLGRDE
                     GLERLWEEVARCCRLDEPDPVAAWTERQDELERIAGELDSRRFDRLHYEGPGTDLTIG
                     LLPSSRWITARFTTAWGLRHMPNIPSEETFTTPDPERVDGVVRSTKPLVRSGTVIRDL
                     EVEFRGGRAVRIEASTGGEGLDAMTAVDDGAARLGEVALVDNDSRVGKSGMVFYDTLL
                     DENASSHIALGRGFPFTVGDDDMERVNHSKVHVDFMVGSDDLVVTGITSSGERVPVLA
                     GGRFQL"
     gene            5857..7038
                     /locus_tag="VF752_11170"
     CDS             5857..7038
                     /locus_tag="VF752_11170"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX8856152.1"
                     /translation="MAGRLATALASVALAAVMLAGCSESEEHKPNSRPKPAPSQPPTR
                     EARTGAAKAGHIPPPASRADVYAAGRPDRLRGPARQALRRVYVPNSDSNTVDVISQES
                     GRVVAHFATGQNPQHVTPSWDLRTLWVLNDQGNSVTPIDPRTGRPGKNVPVADPYNMY
                     FTADGRYAIVVAERLRRLDFRDAETMRMHHALAVPMCAGVDHMDFTADGRRALVSCEF
                     SGRMIEVDLQREKVLKTIDLRSGAMPQDVKLSPDGRRFYVADMASNGVWVLDAHSMRK
                     LRFIHTGMGAHGLYTSRDSRYLYITNRAESSISRLRFSTGKLVGKWKIPGHASPDMGG
                     VSADGKVLWLSGRYNSEVYAISTNTGKLLHRIPVGPGPHGLCVWPQPGRYSIGHTGIL
                     R"
     gene            7077..7415
                     /locus_tag="VF752_11175"
     CDS             7077..7415
                     /locus_tag="VF752_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856153.1"
                     /translation="MRRGQGGVARGTIRGRLEALHDGRLPTPPELLATDEEALRGIGL
                     SRGRFTADGVLMLTLRPRDIWPAADLALRRAVARVWGLDGPLTVAEVDALGERFPPWR
                     TLAAVYLLGA"
     gene            7441..8208
                     /locus_tag="VF752_11180"
     CDS             7441..8208
                     /locus_tag="VF752_11180"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8856154.1"
                     /translation="MQGRVGGWQERQIDALHSRDLDVVCLQEVTPRNRERWEQALAEM
                     GFEVAVSGWPVDPRGSRRFAVLIASRAPAELVQPPALPWQERHLAVRTSIDGAEAEVH
                     TLHAPLSGKEEQVKVRTLEALHEALADSTGVPRVLAGDFNTPQYESRDGEIQTFARTR
                     SGKIRPRYGERHDRAELLLIEDLPKRGWRDAFRAVHGYSRRDRSWVAAVKGYGYRLDH
                     IVVSPELDPVACEYLHEWREAGMSDHSAMWAEIRRAG"
     gene            complement(8192..9112)
                     /locus_tag="VF752_11185"
     CDS             complement(8192..9112)
                     /locus_tag="VF752_11185"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856155.1"
                     /translation="MGAGEHGLNEQFCKAGDVDLCFETFGEPADPAVLLVMGLGTQMV
                     GWNAEFCEGLVDRGFFVIRYDNRDSGRSTHFRDTPPPTSWELMSRRPRNVAYGLSDMA
                     DDGVALLDHLGIERAHVVGASMGGMIAQGVASSHPARVASLVSIMSTTGSRIAGQPAA
                     KVYPFLLGRVPKDKEQFVERAVKLHRVIGSRGFERDENEVREMAEVSHDRGLDPAGAA
                     RQLAAIVAAGNRTAECRRITAPTLVIHGKRDPMIACSGGRATARAIRGSKLMIIEGMG
                     HDLPRGAWPPILDAIAENAARAREPVSPLA"
     gene            9425..10630
                     /locus_tag="VF752_11190"
     CDS             9425..10630
                     /locus_tag="VF752_11190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8856156.1"
                     /translation="MRRLATARFISMAGSDATGVAVGFAMYQQTHSAPWLSLSLMITV
                     GVGALLAPLAGRAGDLFDRKRLMVACEAGAAAIFITLALVHTPIALLGLSVIASAIGT
                     IFGPASGAAIAHVAGERHLSWANAVIATGANVGKTAGRLGAGALIAALGAGSVFVLDA
                     VTFLASAWLIASVRHGFSGPLRGQTASEPDAAAAGESGMRFILEHPKLRLVVASACVS
                     TFATAFSMTAEVPLVFDTGAGALGLGALTASWGTGMVIGSWYGGRALHKGNEATGVLA
                     GRVLMAAGVGFVALSPSIAPMLLCYLLGGIGGGFMGVAAQSLILRVAPDRIRARTLGA
                     IDSARNVTFGLGVVGAGALVQLVGARPVYAMVGLAMAVGTLPVLALVMNLGGPRSLRG
                     VPTVARAAV"
     gene            10640..11056
                     /locus_tag="VF752_11195"
     CDS             10640..11056
                     /locus_tag="VF752_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8856157.1"
                     /translation="MPDLPLTGGCMCGRVRYEVSAPLQSAGYCHCKRCQRRTGAAASA
                     QARVAPGSFRVVQGEDLVHAWRPPGGFEKAFCSECGSALFSRHRDDPSIISVRLGTFD
                     GDPGIRPSTRQWVASAAVWEPIPDDGIPRYDEQRPS"
     gene            11226..11690
                     /locus_tag="VF752_11200"
     CDS             11226..11690
                     /locus_tag="VF752_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8856158.1"
                     /translation="MFKKVVWATDGSESADRALPHIRSMATEGTEVVVLHVDEFVAAP
                     KSGFELHADEEDIQSKIKAQVADLKESGANATLRVVDTRASGAAHAIAEEAAAVGADL
                     VVTGTRGHTPLQGLVLGSVTQRILHIAPCPVLVVPESTSKADRTTESSEAVA"
     gene            11692..12414
                     /locus_tag="VF752_11205"
     CDS             11692..12414
                     /locus_tag="VF752_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8856159.1"
                     /translation="MSGEVLILDDGSGGAPAAASLGEMLAGVLGATPVTVRGEVDHQG
                     VRLIVVPSDDDGRRPHASSVAGRLLHEAHVPVAVAPKGWPGQADGRLHVLGVAYDGSP
                     ESRVALGFARELAERADATVRVYTVAVPARAVSVGAMAGPLPQEEDAREILSRHLEEA
                     VADLPPSLRALPVLLDGDAAKEIAGAAEAGVDLLVTGSRGYGRLGRVLLGSVSSALID
                     TAPCPVIAIPRPAADPATDTGG"
     gene            12476..14914
                     /locus_tag="VF752_11210"
     CDS             12476..14914
                     /locus_tag="VF752_11210"
                     /inference="COORDINATES: protein motif:HMM:NF003621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX8856160.1"
                     /translation="MTRSQAPGPSTPTAEGPLSNAELRAIDAYWRAANYLSVGQIYLL
                     DNPLLRAPLRIEHIKPRLLGHWGTTPGLNLLYAHMNRAIRARGTSTIFVTGPGHGGPG
                     LVANTYLEGTYTEVYPQIGHDEEGLRRLFRQFSFPGGIPSHVAPETPGSINEGGELGY
                     SLLHAYGAVFDNPELLALCVVGDGEAETGALATSWHSTKFVNPASDGAVLPVLHLNGY
                     KIANPTVLARIPEEELRALMEGYGYAPHFVEGSDPDAVHQQLAATLDEVLDEIEAIQT
                     SARSGRNSERPRWPMIVLRTPKGWTGPKEVDGKPAEGTFRSHQVPLADLAKKPEHMRQ
                     LEDWMRSYRPEELFDEAGALVPELAALAPEGELRMGANPHANGGLLRRPLELPDFRRY
                     AVDVPQPGGATAEATRVLGELLRDVMRFNRDNFRLFGPDETASNRLGAVFEAENRTWD
                     AELEPGDDHLAPDGRVMEVLSEQICEGWLEGYLLTGRHGLFNCYEAFIHIVDSMFNQH
                     AKWLKVTRDIPWRRPIASLNYLLSSHVWRQDHNGFTHQDPGFIDHVVNKKAEIVRVYL
                     PPDANCLLSVADHCLRSSNYVNVIVAGKQPALQWLSMDEAITHCTRGAGIWSWASNDE
                     DGETDVVLACAGDVPTLETLAAAALLRQELPDLCVRVVNVVDLMRLVPETAHPHGMSD
                     LEFDSLFTRDKPVLFAYHGYPMLIHRLTYRRHNHENIHVRGYMEEGTTTTPFDMVMLN
                     DLDRFHLVMDVIDRVPGLAERSGQLRQRMFDERLRHRAYTREVGDDPPDVRDWTWPGD
                     GAEPEGGPQRGVPA"
     gene            14914..15963
                     /locus_tag="VF752_11215"
     CDS             14914..15963
                     /locus_tag="VF752_11215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX8856161.1"
                     /translation="MRVLAVNAGSSSIKLSVLDEDGGTLASESLPPDAPIEAFVVAAP
                     DFGAVGHRIVHGGHLHRESALLDDTLERDLGKLADLAPLHNRRGLARVEEVSRLLPGT
                     PQVLCFDTAFHASLPDEASTYAVPWEWRERLGVLRYGFHGLSHDWASRRCAELIGRPR
                     AELRIVSCHLGSGASLAAVARGRSVDTTMGMTPNEGLVMATRSGSFGPGAVMWLLRDA
                     GIGTDELEHTLEHESGLRGIAGTGDMRELLARDDGQARLAVAVYLHRLRASIAAMAAA
                     MEGLDALVFTGGVGEHAPVIRAEACAGLGFLGIVLDTAANERANVDALVGERVAVVTA
                     REDLQIAAEVRRVLS"
     gene            complement(15956..17404)
                     /locus_tag="VF752_11220"
     CDS             complement(15956..17404)
                     /locus_tag="VF752_11220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856162.1"
                     /translation="MEAREVGPLSFVDVAPLALDRFERVLTPEQEQELLRMVARGRVL
                     LEGRVVWSVNSTAHGGGVAEMLQSLIAYARGGGVDARWAVIGGEPDFFRVTKRIHNRL
                     HGYAGDGGPLGEHERAIYDESMAVNAERLASHVQAGDIVLLHDPQTAGLVDAMKTAGA
                     HVVWRAHVGLDSPNDQARDAWRFLLPYVSGADGYVFSRRGFAWEGLDQSRLTIIPPSI
                     DPFSPKNQDLDPDTVRSILHVIGLQRRNGGAPVVTCHDGSSRVVERHAELVEEEPLPE
                     GAPIVTQVSRWDRLKDPIGVMEGFVAHVVPVTDAHLVLAGPETRAVADDPEGAEVLRL
                     CVDRWHGLAPEVRRRVHLAMLPMADGDENAAMVNALQRSAAVVVQKSIAEGFGLTVSE
                     AMWKARPVVASRVGGIQDQIVDGQSGRLVEPTDLESFGAAVAELLLDHEVADHMGKAA
                     MERVRMDFLGARHLGQWVDLFDRVIAATEAVS"
     gene            17554..17958
                     /locus_tag="VF752_11225"
     CDS             17554..17958
                     /locus_tag="VF752_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856163.1"
                     /translation="MKSRSRRLFRYRRHVAVLACAAAMLALPALASARPADEPAVTSQ
                     PATSSFAPSERVTNAAPAFHPSASERVTDYRAPAIYSEPTSTVTVVKHSYDGRTLAIA
                     LSGAALLVALLGSGYVLVRLHGFRQTPAGGIS"
     gene            complement(18047..18637)
                     /locus_tag="VF752_11230"
     CDS             complement(18047..18637)
                     /locus_tag="VF752_11230"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8856164.1"
                     /translation="MNADQASVQTTPVRPKRADARRNYERVLAAAREAFAEGGESTAL
                     EEIARRAGVGIGTLYRHFPTRQALLEALYVNEVEALSHSAAQLDDADPWEALNVWLER
                     LIGYLGTKRALAHELLNYLEEDAALFRECRASLFAAGEPLLKRAQDAGAVRPDVEFSD
                     VMHMVGGISRMPASDSRQTEHVLRIALDGLRYRPQS"
     gene            18701..20302
                     /locus_tag="VF752_11235"
     CDS             18701..20302
                     /locus_tag="VF752_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8856165.1"
                     /translation="MEAPSILAPAGDIDKSREPSSYRYSHIRRPDGPRPGGWLMRQGP
                     LAGRYPAAAAMVIFALVPYLGLSAALQPLTPIIGGQLHMNAETMSLGLGMANAGYAVG
                     TVLAVQFAQLLPQRRMMVLYAALLLIGSVLAAAAQDPAMFIAGHVLQGLCTSLLLIAA
                     VPPLALGFGLDKFRSTAMIMNVCIFGAVALGPVLGGIQAGAHAWRPLFWIVAGISLAA
                     LLLSLLTFEDAPPADPEAPRDLVAIALAASGCVAAFYGASELVTHRFLGTQTFLPLVV
                     GLALIVVLVVNQYRSRRPLLRVRSMLTSTVPVAGIVVALAAAAASVSATVLTASELAD
                     RYSPLHLGLLYLPELGGALITAAMLGVVLNKRQMHYLPLAGMAFLAAGIGVFRVQVPP
                     TEALTLIGSGLTGIGLGAAVAPALFVAGFSLPSASLQRVFAIVELFRAVAAFMIAPVF
                     AHFAVTVGGNARTGTGIALWIGFGLAIGGAIAAVLLYVVGRARPQTPDLERFMAGEAP
                     AWYSPPLLAGIRNRPHHRVLAEDAA"
     gene            20299..20817
                     /locus_tag="VF752_11240"
     CDS             20299..20817
                     /locus_tag="VF752_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8856166.1"
                     /translation="MTQDRDNYPAGPVVFAYDGSALARLAIEEAGRQLAPGRDALVLT
                     VWQPFDVGFVPPVELRIDAAEIADVRNAAEQTAAEGASLAEAAGFKARTTVAETAPSW
                     KGIVQIADEHDASLTVLGSHGRTGLAGVLIGSVAEAVAAHSRRSVLIVHRRDPRDQTE
                     AHANPVTEKDSA"
     gene            complement(21241..23028)
                     /locus_tag="VF752_11245"
     CDS             complement(21241..23028)
                     /locus_tag="VF752_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8856167.1"
                     /translation="MKPTTILATLGAALAAGLATNAAPADAAVSSASIDGQTATLNLD
                     GADDNVTVSVSGGLLVHGQTGGGLNSGSDWDSATPGDQTVPANGTFGVDINGGDGNDS
                     LTVLAKANEIAGAKLNGGAGDDLLTGADTADSLGGGDGNDRLVGAKGGDDMSGGAGND
                     TLVWNNGDGSDMIDGDAGNDTTEVNGNPTLGDTFTLEPNAGRITFKRTNLVTFTLNAS
                     TERFQVNGLGGDDSVSASNGVGALTLLSVDGGAGNDTVNGSDGPDLLVGGEGNDVLGG
                     GAGDDRIVGDRGSDTMNGGAGDDTLVWNNGDGSDVMNGDDGRDDVEVNGSPTAGDVFT
                     VQPNGARIKFDRTNLVPFSLDIGSSETMHANGLAGDDTITVGEVGSFAVTAAGGAGND
                     TLTGRGSSETFLGGSGNDTIAPGGGIDFVSGDEGDDHVNVRDKTADLAVGGAGNDSVV
                     ADAGRLDILDGFEAVDRTPNATPRPGDRSTRPVTIKGGTLRVSKGRASVRASCSANSS
                     ANCTGSLSLRTAKRVRFAGVSAVLQLGSARYHVARGTTKSITVKLPKGIQRLADRNGR
                     LKVVAVASTRGSGSIASSSRRATLALRTH"
     gene            23144..23632
                     /locus_tag="VF752_11250"
     CDS             23144..23632
                     /locus_tag="VF752_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8856168.1"
                     /translation="MARTLDPESLGDHLDRLFRAAWALCGSREDAEDLVQETYERVLR
                     RPRFVRRDDDLGYLYRTLHNTYFNARRTASRRPRVTAQLDDVQAASSRADVQPEQAAE
                     NNLVLEAVAALSQPMREVLVAVDVLGLSYAEAGKALGVHENTIPSRLARARLEVAEAL
                     QG"
     gene            23684..>23942
                     /locus_tag="VF752_11255"
     CDS             23684..>23942
                     /locus_tag="VF752_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856169.1"
                     /translation="MEPEPTKRLSDDELADVARLVDGTLPADRRAEVEARVAASPQLS
                     SIVERQRAAVDALRGTAEIGAPQQLRSRVDRRRGKGRATDRG"
BASE COUNT         3437 a         8432 c         8267 g         3806 t
ORIGIN      
        1 cgaggaaggc gctcttcgcc tcagcggcgg cctcggcatc gcgccgcgcc tggaggaggt
       61 cgctgatgtc gtggtagagc gcgtagtacc ccacgtgttg ctcgcccacg aagatcggcg
      121 cgcctcgcac gtcgacatcc acgaggctcc catccttgcg cgtccgctga gtggtgatgt
      181 gtaggtcctc gccctttctg gccgagtagg cggcgtcgat cgcctcggcg cgcaccgcgt
      241 ccgatcgagc gacgaggtcg tcgacgtcgc ggccgatcgc ctcctggcgg tcgtatccga
      301 agagcttctc ggccgccggg ttccacgagg tcacgttgcc gtcgaggtca accgtgacga
      361 tcgcggtcgg gctgagctcg acgagcgact cgaagtactg cttctggcgc cggacctccg
      421 actcactctg gcgaagcgcc tcctcggcaa gcttccgctg ggtgatgtcc tcgaccatgg
      481 tgaggccgaa cagcgcctcg ccctggtcgt cgcgcacgat cgagtcgaca acctgagccc
      541 agatggtccg cccgtcgcga ccgatgtagc gcatctcgag ctcgtatcgg tcggccttcc
      601 cagccacgag atcgcggtag agcgcaagct gccgcggccg gtcctccgga tgcgtgtact
      661 cgctcgggtg gagcgtctga agctcatcct cgctgtagcc cagcatctcc cggtaggcgc
      721 ggttccaccg gaactcgccc tcgccaccgg ggcgccggag aaacgtggga accagcattg
      781 tgctgacgtc gagcgggccc caggcgaccc cgatcggcgc ctcctcgaac atcacgcgga
      841 accgctgctc gctctcgcgc agcgcctcct ccgcacgctt gcgcttgcgc acgtcgtggt
      901 agatcgcata cgtgcccacc ggctctccgc ggagcagcac aggcgcggcc agcagctcga
      961 cgtcgatcag cgagccgtcc ttgtgggtgc gcttggtgaa ggcctgcacg cggccctccg
     1021 tgtaggcgtc gcgcgtgacc gaggtggcct ctgcgcgaag ctccggcgac ttcgcaacga
     1081 ggtcatcgat cttgcggccg tgcgcctcgg ccggcgtgta gccgaacagc ctctcggccg
     1141 cggggttcca cgaggtgacc acgtcctcga ggtccgtcgt gacgatcgcg accgggctga
     1201 tctcgagcag cgactcgaag tagcgcttct gcctcccgag ctcctccgta cgtgcagcga
     1261 gcgcctcctc gagctccttc cgctcggtga tgtcgaggta gaagccttgg tgcaacgcgg
     1321 gcatgccggc gtcgcctggt aggacggtcg cctgatcgag gaaccagacg acgcgcccgt
     1381 cgcgcgcgat caggcggtac tcctcccgga agggctcgcc agtctcgcgc gagcggttat
     1441 gcgcctccat cacccgctca cggtcgtcgg gatggaggat cttgagcagg tggcggtcgt
     1501 cgcttgccca ctcctccgtc gtgtagccca ggatcgcttc gagctgcggg ctcgtgtaga
     1561 cgttcgagct ccactcgtcc aggcggtcga tgtagacggc cagggggagc tgttcgatca
     1621 gcgcccgcca accgtgtgag cgcaagtcca gatcaactag cgcccccgag ttgcgagcgc
     1681 cacgacgccc cggagtcgtg tgctccgggt tatgagccat accctgacga gcatacgctc
     1741 gcgggtaggc gccgacaacg gaccctgccc gggtggggac gctacgcccg gatctccgcc
     1801 tcggcgttgc ggatgatctg gttgccctcc tgctcggcca cggtgtcgat cacgagcacg
     1861 ccatcgcgct cctcgcgaac cgtgcccgtc acgacgatct ccttctccgg gagcgccatg
     1921 ccccggaact ggaccgacag gcgcttgagc gcccgcgggt cgccgcccgc cgcctgcgtc
     1981 tgggcgcgcg ccacctgcgc catcccgtag agaccgtgca ggatgttcga cggaagcccg
     2041 accgcctggg cgaactcggg atcgatgtgg atcgggttga agtcgcccga cgctcccgcg
     2101 tagcggtgcg gcaggtactt gtcgggcgtc acgcgcagct cgggaatctg ctctccctgt
     2161 ttcatctcag cctcctcgga cgatgttggt ccaggtaccg cgcacgacct cctggccgtc
     2221 ctggttctcc gacaccgact cgaacacgta gaacgtcttg tcgtccttct cgaagatgtc
     2281 cttcaccgtg gtggtcgtgg tgatggtgtc gctcgccacg acgggctcgc cccagacgaa
     2341 ctcctggctg ccgtggagca tcatcgcgaa attgatcccg acctcgggat cgagcacggc
     2401 cggccctacc gagcctgcgg agtacacgac cgcgaacatc ggaggcgcca ccacgttgcg
     2461 gaagcccttc tcgcgcgcgg cggccgggtc gtgatggacc gggtcggact cgccgaccgc
     2521 gtatgcgtac tcgcggatct tctcccgccc cacctcgtac tgatggggcg gataggactt
     2581 cccgatgatg ctcgtgtcga ctgccaaccg gcctcctcgc gggatcgctt gcgcgcggca
     2641 gtctaaggtg cgccgacctt gcgaaggctg ctcgtcattg gcgccgcgct gctcgcggcc
     2701 tcgcccgcgg cacacgcggc tcggctcgag cgggtcgccc acttccggca gccggtctac
     2761 ttgacctcca gcccctcgga caggcgcgac gtgttcgtgg tcgagcgcgc cggccgggtg
     2821 ctggcgatcc gcaacgggcg cgtgctgcgg cggccggtgc tcgacatccg caggcgcgtg
     2881 ctcatcgccg atagacgtat ctacaaggac cagggcgggt tgctctccgt cgcgctcgcg
     2941 cccgactacg accgcaccca tcgcctctac gcgttcttca ccgcgcgcga caagacgatc
     3001 cgcgtcgagg agttccccac ggggcgcacg ctcgtggccg tggcgcgcac ctcgccgttc
     3061 gacgcgggtg ggcagctcca gttcgggccg gacggcgcac tctacatcgg catgggctac
     3121 ggcggtggca acagcagggt cgacgggagc gcgctcgacc ccgcctcgcc gcgcggcaag
     3181 atcctgcgct gggatcccgc gagcccgaca gcgcctccgg tcccttattt gctcgggctt
     3241 cgcacgccgt ggcgcttctc gttcgaccgc ggcaatctga tcgtgggcga cgtgggcgag
     3301 agccgtcgcg aggaggtgga cttcctgccg aggggccggc aggacggcgc gaacctcggc
     3361 tggcccgcgt tcgagggcac gctcaggcac ggcccgttcg cggcgcccgg cgcaattcca
     3421 ccggtcctgc agaagcggca cagctccggc tggtgcgcac tgatcgccgg ttacgtggtt
     3481 cgagatcgct tgctgcacga tctctacggc cgctacgtgt acggcgacct ctgcaagagc
     3541 gggatccgct cggtgcggct ctctgcgcgc ggggcgcggg ccgaccggtc agaacgcctc
     3601 gcggtgccca acctcgtctc gttcggtgag gacgcacgcg ggcacttgta cgcggtgagc
     3661 ctcgacggct gggtctaccg gttcgccggc tgacgccagg catcgtagac cgcggccatg
     3721 tcgtcgtcgg cgtggccctc ggatatcgcc ttctcgaact gccgcgcgac ggtctcggcg
     3781 atcggcagga caaggccgtg gcgttcggcc gcctcgagca cgagctcggc gtccttgagc
     3841 gccaagcgag ccgggaagga cggatcgaag cggtcgtcga tcatcatctc gcccttcagc
     3901 cgcgcgtagg cggggccgag cgggccgccg tcgatcgcgt caaggaagtt gcgtgggtcg
     3961 acgtcgatct cctgcgcgaa ggcgatcgtc tccgccaaag tgcccaccag tccgagcagc
     4021 cagttgttga tcacgagctt caacctcgtg cccgcgccgg cgtcaccgac gcgtatcgtc
     4081 cgcgacccga ccgcctcgaa gatcggtgcg cagacgtcga gcgcctcgtc cggcccggaa
     4141 gccagcacga tcagctcgcc cttctcggcg ggctccttgg tgccgagcgc cggcgcatcg
     4201 acgagcgtca cgcggtgctc gtcggcaagc gcggcaaccc gctctgtagc cgagatgccg
     4261 atggtgctca tctgatccca gacggcgtcg tcgcgcatcg ccgggatggc ctccttcatc
     4321 acggacagca cggcgtcacc gtcggcgagc atggtcacca ccacgtcggc gctttcgact
     4381 gcctccgcgg gcaagccggc gatgtcgatg ccgtcgcccg cgatcgcgtc agctttttca
     4441 cgcgagcgat tccacgcgcg gacgcgcata ccggcgcccg cgatgtgccg cgccatcggc
     4501 gcgcccatga tgccggtgcc gagcaccgcg acggtctgtg gcggttcggg cataccgaca
     4561 cgcagttgcc ccagaccctg ccggcgctaa acgggatcag agctggaagc gaccacccgc
     4621 gagcaccggc acgcgctcgc ccgacgaggt gatgcccgtc acgaccagat cgtcgcttcc
     4681 gaccatgaag tccacgtgca ccttcgagtg gttcacgcgc tccatgtcgt cgtcgccgac
     4741 cgtgaacgga aagccgcggc cgagggcgat gtggctcgac gcgttctcgt cgagcagcgt
     4801 gtcgtagaag accatccccg acttcccgac gcggctgtcg ttgtccacca gcgcgacctc
     4861 gccgagccgc gcggcgccgt cgtcgacggc ggtcatcgca tcgagcccct ctccgccggt
     4921 cgaggcctca atccgcaccg ctcgcccacc acggaactcc acctcgaggt cgcggatgac
     4981 ggtgccggag cgcaccagcg gcttggtgga gcggacgacg ccgtccacgc gctcgggatc
     5041 gggtgtggtg aaggtctcct ccgacggaat gttcggcatg tgccgcagcc cccacgccgt
     5101 cgtgaagcgg gcggtgatcc agcgtgagct cggaaggagc ccgatggtga ggtcggtgcc
     5161 cggcccttcg tagtggagac ggtcgaagcg gcgcgagtcg agctcgccgg cgatccgctc
     5221 gagctcgtcc tggcgctctg tccacgccgc caccggatcc ggctcgtcca ggcggcagca
     5281 gcgcgcgacc tcctcccaga ggcgctcgag cccctcgtcg cggccgaggt ccggatagac
     5341 gagggctgcc cagtcgggcg tcgggcacgg caccgcggtc cagttcaccg agcgctctga
     5401 cacgaccacc gcccactcct cgagggcggg ccacgtgtcc ttgcccaccc gctcggggtc
     5461 gacgtcctcc agcaggtccg gagcgaccgc gcccgtgagg cccacgcttg caccgtgttg
     5521 gcgcccgagc tcgagcaccc gctcccccag ccaggagggc acgaagccga tcgcatcctc
     5581 tgagccgtgg agcagccgcg cccgcttcac gtacgcgtcg tacgtcgcga cgtcgacgaa
     5641 cttcgcgccc gctcggtagg ccgcgtcggc gaccgcgcgc gcgagcggct ccttgccggg
     5701 ctcggtgctg acggccacca cctgcccctc ctgcacgttg gcgccgaagc ggacggcgag
     5761 atccgcgagg cgccccaccc agtcctcggt catggcggac gattgtgtca gggcttgagg
     5821 agtgcttcac ggagccctgg gaacaatgcg cgacggatgg cgggacgcct cgcgacagcc
     5881 ctcgcttccg tcgcgctcgc tgccgtgatg ctcgccggct gctcggagag tgaggagcac
     5941 aagccgaact cacggcccaa gccagcgccc tcgcagccgc caacgcggga agcgcgtact
     6001 ggcgccgcga aggccggtca cattccgccg cccgcgtcgc gcgccgacgt gtacgccgcc
     6061 ggccgcccgg accgcctccg cgggccggcg cgccaggcgc tcaggcgcgt gtacgtgccg
     6121 aattcggact cgaacacagt cgatgtgatc tcacaggaga gcgggcgcgt ggtcgctcac
     6181 ttcgccaccg gacagaaccc tcagcacgtg acgccctcgt gggacctgcg cacgctctgg
     6241 gtgctgaacg accagggcaa cagcgtcacc ccgatcgacc cgcgaaccgg tcggcccggt
     6301 aagaacgtgc ccgttgccga cccgtacaac atgtacttca cggcggacgg gcgctacgcg
     6361 atcgtggtgg ccgagcggct caggcggctc gatttccgcg acgcggagac gatgcggatg
     6421 caccacgcgc tcgcggtgcc tatgtgcgcg ggcgtcgacc acatggactt caccgccgac
     6481 ggccgccggg cgctggtgtc gtgcgagttc agcgggcgca tgatcgaggt cgaccttcag
     6541 cgcgagaagg tgctgaagac gattgacctt cgtagcggcg cgatgccgca ggacgtgaag
     6601 ctctcgcccg acggccggcg cttctacgtg gccgacatgg cgtcgaacgg agtctgggtc
     6661 ctcgacgccc acagcatgcg aaagcttcgc ttcatccaca cgggcatggg cgctcacggc
     6721 ctctacacca gccgcgactc gcgctacctc tacatcacga accgtgccga gagctctatt
     6781 tcgcggctgc gcttctcgac cggaaagctc gtgggcaagt ggaagatccc cgggcatgca
     6841 agcccggata tggggggagt gtcggcggac ggaaaggtgc tgtggctgtc cggccgctac
     6901 aactccgagg tctacgcgat ttcgacgaac accgggaagc tgctgcatcg gataccggtc
     6961 gggccaggac cgcacggcct ctgcgtctgg ccgcagcccg ggcgctactc gatcgggcac
     7021 acgggcatcc tgcgctgacc aggttctccg ggagcgaccc ggttgagcgc cgaccggtgc
     7081 gcagagggca gggcggtgtt gcgcgcggga cgatccgggg ccggctcgag gcgctgcacg
     7141 acggccggct gccgacgccc ccggagctgc tcgccaccga cgaggaggcg ctgcgcggga
     7201 tcgggttatc ccgcggccgg ttcacggccg atggcgtgct gatgctcacg cttcgcccgc
     7261 gcgacatctg gcccgccgcg gacctggcac tgcgccgcgc ggtcgcgcgc gtttgggggc
     7321 tcgacggccc gctaacggtc gccgaggtgg acgccctcgg cgagcgcttc ccgccctggc
     7381 gcacgctggc cgccgtgtac ctcctggggg cctaacctgg ccgagctgct cacctggaac
     7441 gtgcaggggc gggtcggcgg ctggcaggag cggcagatcg acgccctgca ctcgcgcgac
     7501 ctcgacgttg tgtgcctcca agaggtgacg ccccgcaacc gcgagcgctg ggagcaggca
     7561 ctcgccgaga tgggcttcga ggtggccgtg agcgggtggc ccgtcgaccc gcgcggctcg
     7621 cgccgcttcg cggtgctgat cgcgagccgc gcgccggccg agctggtcca gcctccggcg
     7681 ctgccatggc aggagcgcca cctcgccgtg cgcacgagca tcgacggcgc cgaggcggag
     7741 gtgcacacgc tgcacgcgcc gctcagcggc aaggaggagc aggtgaaggt gcgcacgctc
     7801 gaggctcttc acgaagcgct cgctgattcg acgggcgtgc cgcgcgtgtt ggcgggcgac
     7861 ttcaacacac cccagtacga gagccgcgac ggcgagatac agacgttcgc gcggacacgg
     7921 agcggcaaga tccgtccgcg ctacggcgag cgccacgacc gggccgagct gctgctcatc
     7981 gaggacctcc caaagcgcgg ctggcgcgat gccttccgcg cggtgcacgg ctattcacgg
     8041 cgtgaccgct cgtgggtggc cgcggtgaag ggctacggct accggctcga ccacatcgtc
     8101 gtatcgcccg agctcgatcc cgtcgcgtgc gagtacctgc acgagtggcg cgaggccggc
     8161 atgtccgacc actcggcgat gtgggccgag atcaggcgag cgggctgact ggctcgcggg
     8221 cgcgcgccgc gttctcagcg atcgcgtcga gaatcggcgg ccaggctccg cgcggcaggt
     8281 cgtggcccat gccctcgatg atcatcagct tcgagccgcg aatcgcgcgg gccgtggcgc
     8341 gaccgcccga gcaggcgatc atcgggtcgc gcttgccgtg gatgacaaga gtgggagccg
     8401 tgatcctgcg gcactcggcg gtgcggttgc ccgccgcgac gatcgccgcc agctggcgcg
     8461 cggcgccggc cgggtcgagg ccgcggtcgt gggagacctc ggccatctcg cgcacctcgt
     8521 tctcgtcacg ctcgaagccg cgggacccga tcacgcggtg gagcttcacg gcccgctcca
     8581 cgaactgctc cttgtccttc ggcacgcgcc cgagcaggaa cggatagacc ttggccgcgg
     8641 gctgcccggc gatgcggctg cccgtcgtcg acatgatcga caccagcgag gcgacgcggg
     8701 ccgggtggct cgatgcgacc ccctgggcga tcatcccgcc catcgaggca ccgaccacgt
     8761 gcgcccgctc gatgccgagg tggtcgagta gggcgacgcc gtcgtcggcc atgtcggata
     8821 ggccgtaggc gacgttgcgc gggcgccggc tcatgagctc ccacgacgtg ggaggcggcg
     8881 tgtccctgaa gtgcgtcgag cggccgctgt cgcggttgtc gtaccggatc acgaagaagc
     8941 cgcggtcgac caggccctcg cagaactctg cgttccaccc gaccatctgc gtgccgagcc
     9001 ccattacgag gaggacggcg gggtcggccg gctcgccgaa cgtctcgaag cagaggtcga
     9061 cgtcccctgc cttgcagaac tgctcgttca ggccgtgttc gcccgcgccc acggcgctgg
     9121 actgtactga gtaggtcaac cggtcccggc cggatcccac cgtcgcgtga gtttgcgggg
     9181 agaagtggtc gcggtggacc aggtttccta gattgaggtg cttgcccgtg caaccagttg
     9241 cggggcttcg aattggatgg gcgtgtgcgc ctcccgcgtg ggagccgcgc gcacctgtga
     9301 cgacatatga actggccgag tggcgcccag cgcgcccgag gcggcgccgg ccaggaagga
     9361 atagatgctc ccccctctcc cccttgggcg cgcccgtctg ggcggcgacc cggcgcgcgg
     9421 tgccgtgcga agactcgcga ccgcgcgctt catatcgatg gccggcagtg acgccaccgg
     9481 cgtcgctgtc ggattcgcga tgtaccaaca gacccactcg gcgccctggc tctcgctatc
     9541 gctcatgatc acggtgggcg tgggcgcgct gctcgcgccg ctggccggcc gcgccggtga
     9601 cctcttcgac cgcaagcgac tgatggtcgc ctgcgaggca ggcgctgccg cgatcttcat
     9661 tacgctggcg ctggtgcaca cgcccattgc gctgctcggc ctgagcgtga tcgcctccgc
     9721 gataggcacg atcttcgggc ccgcctcggg cgctgcgatc gcccacgtcg cgggcgagcg
     9781 gcacctgtcg tgggccaacg ccgtgatcgc caccggcgcc aacgtcggca agaccgccgg
     9841 gcggcttggt gccggcgcgc tgatcgccgc gctgggcgcg ggcagcgtgt tcgtgctcga
     9901 cgcggtcacg ttcctggctt cagcgtggct gatcgcgtcg gttcggcacg ggttctcggg
     9961 gccgctgcgt ggccagacgg catccgagcc tgacgcggcc gccgccgggg agagcggcat
    10021 gcggttcatc ctcgagcacc cgaagctgcg gctcgtggtc gcctccgcct gcgtttccac
    10081 gttcgccacg gcgttctcca tgacggccga ggtgccgctg gtgttcgaca ccggcgcggg
    10141 cgcgcttggc ctgggtgcgc tcaccgcgag ctggggcacc ggcatggtga tcggctcgtg
    10201 gtacggcggg cgcgcgctgc acaaaggcaa cgaggcgacc ggcgtgctcg caggccgcgt
    10261 gctcatggcc gccggcgtgg gattcgtcgc gctttcgccc tcgatcgcgc cgatgctcct
    10321 ctgctacctg ctcggcggca ttggaggcgg cttcatgggt gtcgcggcgc agtcgctaat
    10381 cctgcgagtc gcgcccgatc gcatccgcgc gcggacgctg ggcgcgatcg actcggcacg
    10441 caacgtcacg ttcggcctcg gcgtcgtcgg cgctggcgcg ctcgtgcagc tcgtcggggc
    10501 acgaccggtg tacgcgatgg ttggactcgc gatggcggtc ggcacgctgc cggtgctggc
    10561 gctcgtgatg aacctgggcg gcccacgctc actgcgcggc gtgccgaccg tggcacgcgc
    10621 cgcggtgtag tttgggcgcg tgcccgatct accgctcacg ggcggctgca tgtgcggccg
    10681 cgtgcgctac gaggtgagcg cgccgctcca gtcggcgggc tactgccact gcaagcgctg
    10741 tcagcggcgc accggcgctg ccgcgtcagc gcaggcgcgc gtggcccccg gctcgttccg
    10801 cgtcgtgcag ggcgaggacc tggtgcacgc gtggcggccg cccggcggct tcgagaaggc
    10861 attctgctcg gagtgcggat ccgcgctgtt cagccgccac cgcgacgatc cctcgatcat
    10921 cagcgtgcgg ctcgggacgt tcgacggtga cccgggaatc cggccctcca cgcgccagtg
    10981 ggtagcgtcc gcggcggttt gggagccgat ccccgacgac ggcatcccgc gctacgacga
    11041 gcagcgtccg tcctagggcc agcccgccaa gcaccacccc gccgacggcg agctgattcc
    11101 gggttggtgg gacgactgag actgctcagt agcttgtact gagcatgcac cggactcggc
    11161 caccttgccg ccggctccct gcgcgcggat gatccgcttg tcccgcctgc gagaagggag
    11221 aacagatgtt caagaaggtc gtctgggcga ccgacggctc cgaatccgcc gaccgagcgc
    11281 ttccccacat ccggtcgatg gctaccgagg gaacagaggt agtggtgctg cacgtcgacg
    11341 agttcgtcgc cgccccgaag tctggtttcg agctccacgc cgacgaagag gacattcaga
    11401 gcaagatcaa ggcgcaggtc gctgacctca aggagtcagg cgcgaacgcc acgttgcgcg
    11461 tggtggacac ccgtgcgagc ggcgcagcgc acgcgatcgc cgaggaggcc gccgcggtgg
    11521 gcgccgacct cgtcgtgacg ggaactcgcg ggcacacgcc actccaaggg ctcgttctcg
    11581 gcagcgtgac ccagcgcatc cttcacatcg cgccctgccc ggtgctggtg gtcccggagt
    11641 cgacgtccaa ggccgaccgc actaccgagt cgagcgaggc cgtcgcctga cgtgtccggc
    11701 gaggtcctga tcctcgacga cggcagcggc ggcgcgccgg ccgccgccag cctcggagag
    11761 atgctcgccg gcgtgctggg agcaacgccc gtgacggtgc gcggcgaggt ggaccaccag
    11821 ggcgtccgcc tgatcgtggt tccgagcgac gatgacggcc ggaggccgca cgcgagctcg
    11881 gtcgccggcc ggctgcttca cgaagcgcac gttccggtcg cagtggcgcc gaagggctgg
    11941 cccggacagg ccgacggccg cctccacgtg ctgggcgtcg cgtacgacgg ctcgcccgag
    12001 tcgcgggtgg cgctcggctt cgcccgcgag ctcgctgaac gagcggatgc gacggtgcgg
    12061 gtctacaccg tggccgtgcc cgcacgcgcg gtatccgttg gggcgatggc cggtcccctt
    12121 ccgcaggagg aagatgcacg ggagatactg agtcggcacc tcgaggaggc cgtggcggac
    12181 ctaccaccgt ccctccgcgc cctgcccgtg ctgctcgacg gcgacgcggc gaaggagatc
    12241 gcgggcgccg ccgaggcggg cgtcgatctg ctcgtgacag gctctcgtgg gtacggccgg
    12301 ctcggccggg tgctgctcgg ctccgtctcg tcggccctca tcgacacggc gccgtgtccc
    12361 gtcatcgcta ttccgcgacc ggcggccgac ccggctaccg atacgggcgg atgagctgcg
    12421 gaccgcgcca gaggcggcgg atggggcggc tggcgtccgg gtacggtaga ccgcggtgac
    12481 ccgctcgcag gcacccggcc cgtccacgcc tacggcagag ggcccgctct cgaacgccga
    12541 gctgcgcgcg atcgacgcgt actggcgcgc ggcgaactac ctgtcggtcg ggcagatcta
    12601 cctgctcgac aaccctctgc ttcgagcgcc gctgaggatc gagcacatca agccgcggct
    12661 gctcggccac tggggcacga cgccggggct gaacctgctc tacgcgcaca tgaaccgcgc
    12721 cattcgagcg cgcggcacga gcacgatctt cgtgaccggt cccggccacg gcggaccggg
    12781 cctcgtcgcg aacacctacc tcgagggcac ctacaccgag gtgtatccgc agatcggcca
    12841 cgacgaggag ggactgcggc ggctgtttcg ccagttctcg ttccctggtg ggatcccgag
    12901 ccacgttgcg cccgagacgc ccggctcgat caacgagggc ggcgagctcg gctactcgct
    12961 cctccatgcg tacggcgcgg tgttcgacaa cccggagctg ctcgcactct gcgtggtggg
    13021 cgacggcgaa gccgagaccg gcgcgctcgc cacgtcgtgg cactcgacca agttcgtgaa
    13081 ccccgccagc gacggcgcgg ttctgcccgt cctccacctg aacggctaca agattgcgaa
    13141 ccccacggtg ctcgcgcgca tccccgagga ggagctgcgc gcgctgatgg agggctacgg
    13201 ctacgcgccc cacttcgtgg agggctcgga ccccgacgcg gtgcaccagc agctggccgc
    13261 cactctcgac gaggtgctcg acgagatcga ggccatccag acctccgcgc gttcgggacg
    13321 gaacagtgag cggccgcgct ggccgatgat cgtgctgcgt acgccgaagg gctggacggg
    13381 tccgaaggaa gtcgacggca agccggcgga gggcacgttc cgctcgcacc aggtgccgct
    13441 tgcggatctg gcgaagaagc cggagcacat gcgccagctc gaggactgga tgcggagcta
    13501 ccgccccgag gagctcttcg atgaggccgg cgcgcttgtg cccgagctcg ccgcgctcgc
    13561 acccgagggc gagctgcgca tgggcgcaaa cccgcacgcg aacggcgggc tgctgcggcg
    13621 gccactcgaa ctgccggact tccggcgcta cgccgtcgac gtgccccagc ccggaggcgc
    13681 cacggccgag gccacccgag tgctcggcga gctgctgcgc gacgtgatgc gcttcaatcg
    13741 cgacaacttc cggctattcg gtcccgacga gactgcgtcg aaccgcctcg gcgcggtgtt
    13801 cgaggccgag aaccgcacat gggatgccga gctcgagccg ggtgacgacc acctcgctcc
    13861 ggacggacgg gtgatggagg tgctcagcga gcagatatgc gagggctggc tcgaggggta
    13921 cttgctcacc ggccggcacg ggctcttcaa ctgctacgag gcgttcatcc acatcgtcga
    13981 ctcgatgttc aaccagcacg cgaagtggct caaggtgacg cgcgacatcc cctggcggcg
    14041 gccgatcgcg tcgctcaact acctgctgag ctcgcacgtc tggcggcagg accacaacgg
    14101 cttcacgcac caggatccgg ggttcatcga ccacgtggtc aacaagaagg ctgagatcgt
    14161 gcgcgtttac ctgccgcccg acgcaaactg cctgctgtcg gtcgcagacc actgcctccg
    14221 cagcagcaac tacgtgaacg tgatcgtggc cggcaagcag ccggcgcttc agtggctctc
    14281 gatggacgag gcgatcacgc actgcacgcg cggtgcgggc atctggagct gggcatcgaa
    14341 tgacgaagac ggcgagacgg acgtggtgct cgcctgcgcg ggcgatgtcc cgacgctcga
    14401 gacgctcgcc gccgcggcac tgctgcggca ggagctgccg gacctgtgcg tacgggtggt
    14461 caacgtcgtc gatctgatgc gcctggtgcc ggagacggcg cacccgcatg ggatgtccga
    14521 cctcgagttc gactcgctat tcacgaggga caagccggtg ctgttcgcct accacggtta
    14581 tccgatgctc atccaccgcc ttacgtaccg ccgccacaac cacgagaaca tccacgtgcg
    14641 cggctacatg gaggagggca cgacgaccac gccgttcgac atggtcatgc tcaatgacct
    14701 cgaccgcttt cacctggtga tggacgtgat tgaccgggtg ccgggtctcg ccgagcgctc
    14761 cgggcagctg cggcaacgca tgttcgacga gcggctgcgc caccgtgcgt acacgcgcga
    14821 ggtgggcgac gacccgcccg acgtgcgcga ctggacctgg ccgggcgacg gcgcggagcc
    14881 ggaaggcggg cctcagcgcg gcgtgcccgc gtagtgcggg tcctggcggt caacgcaggc
    14941 tcgagctcga tcaagctgag cgtgctcgac gaggacggcg gcacgctcgc gtccgagtcg
    15001 ctcccacccg acgcgccgat cgaggcgttc gtcgtcgcgg caccggactt cggcgccgtc
    15061 ggccaccgga tcgtgcacgg aggacacctg caccgcgaga gcgcgctgct cgacgacacg
    15121 ctcgagcgcg acctcggcaa gctcgcagac ctcgccccct tgcacaaccg gcggggcctt
    15181 gcgcgcgtgg aggaggtgag ccgtctgctg cccggcacgc cgcaggtgct gtgcttcgac
    15241 accgccttcc acgcgagcct gcctgacgag gcgtccacct atgcggtccc gtgggagtgg
    15301 cgcgagcggc tcggcgtgct ccgctacggc ttccacggcc tctcgcacga ctgggcgagc
    15361 cggcgctgcg ccgagctcat cggccggccg cgtgcggagc tgcgcatcgt gagctgccat
    15421 ctcggctcag gcgcctcgct cgcggccgtc gctcgcgggc gctcggtcga cacgacgatg
    15481 ggcatgacgc ccaacgaggg gctcgtcatg gccacgcgct cgggctcctt cggcccgggc
    15541 gcggtgatgt ggctgctgcg cgacgcaggc atcggcacgg acgagctcga gcacaccctc
    15601 gagcacgagt ccgggctgcg tggcatcgcg gggacaggcg acatgcgcga gctgctcgcg
    15661 cgcgacgacg ggcaggcgcg cctcgccgtg gcggtgtacc tgcaccggct gcgcgcttcg
    15721 atcgcggcga tggctgctgc catggaaggg ctcgacgcgc tcgtgttcac gggcggtgtt
    15781 ggagagcacg cgccggtgat ccgggcggag gcctgcgcgg gcctcggctt cctcgggatc
    15841 gtgttggata cggccgccaa cgagcgggcg aacgtggacg cgctcgtcgg cgagcgcgtc
    15901 gctgtcgtta ccgcgcgcga ggatctgcag atcgcggcgg aggtgcgccg ggtgctcagc
    15961 tgaccgcttc ggtcgccgcg atcacgcggt cgaaaaggtc gacccactgg cccaagtggc
    16021 gcgcgccaag gaagtccatc ctcacgcgtt ccatggccgc cttccccatg tggtcggcga
    16081 cttcgtgatc gagtagaagc tccgccaccg ccgcgccgaa gctctcgagg tcagtcggct
    16141 ccaccaggcg cccgctctgc ccgtccacga tctggtcctg aatcccgccc acccggcttg
    16201 cgacgaccgg ccgtgccttc cacatcgcct ccgacacggt caggccgaag ccctcggcga
    16261 tgctcttctg gacgaccact gcggccgagc gctgcagcgc gttcaccata gccgcgttct
    16321 cgtccccgtc cgccatcggc agcatcgcga gatggacgcg ccggcgcacc tcgggcgcca
    16381 gcccatgcca gcgatcgacg cagagccgca gcacctcggc gccctcggga tcgtccgcga
    16441 ccgccctggt ctcgggaccc gcaagcacca gatgcgcgtc ggtgaccggg acgacgtggg
    16501 cgacgaatcc ctccatcact ccaatcgggt ccttgaggcg gtcccaccgt gacacctgcg
    16561 tgacgatcgg agctccctcg ggtagcggct cctcctcgac gagctcggcg tgccgttcca
    16621 cgacgcggct gctgccgtcg tggcacgtga ccaccggcgc accgccgttg cggcgctgca
    16681 gcccgatcac atggaggatc gaccgcacgg tatccgggtc caggtcctgg ttcttcggcg
    16741 agaagggatc gatggagggc gggatgatcg tcagacggct ctggtcgagg ccctcccagg
    16801 cgaagccgcg acgcgagaag acgtagccgt cagcgccgct cacatatggg aggagaaagc
    16861 gccaggcgtc gcgtgcctgg tcgttcggag agtcgaggcc cacgtgcgcg cgccacacaa
    16921 cgtgggcgcc cgccgtcttc atcgcatcga cgagcccggc cgtctgcggg tcgtgcagga
    16981 gcacgatgtc gccggcctgt acgtgcgagg ccagccgctc cgcgtttacc gccatggact
    17041 cgtcgtagat agcccgctca tgctcgccga gcggcccgcc gtcgccggca tagccgtgga
    17101 gccggttgtg gattctcttc gtgacgcgga agaagtcggg ctcgcctccg atgacggccc
    17161 aacgcgcgtc gactccgccg ccgcgggcgt acgcgatcaa cgactggagc atctcggcaa
    17221 ctccgccccc atgggcggtc gagttgacgc tccagacgac gcggccctcg agcaggacac
    17281 ggcccctggc aaccatgcgg agcagctcct gctcctgctc cggcgtcagc acacgctcga
    17341 accgatcgag agcgaggggt gcgacgtcaa cgaagctgag cgggccgacc tcacgcgcct
    17401 ccatgaggag acgctacccg gtttcggggc cggcgggtct tcgccgccca ctgctctgtg
    17461 cacgattact cactcggcgc gtgacgcgct tcgcggaacg gccctcctag ggagcgtagg
    17521 ttgctcgcga ctcgccaccg taggaggtcc caaatgaagt cccgcagtcg ccgtctcttc
    17581 cgttaccgcc gccacgtcgc cgtgctcgct tgcgcggcgg cgatgctggc acttcccgcg
    17641 ctcgcctccg ctcgccctgc cgacgagccg gctgttacgt cacagccagc gacgtcgagc
    17701 ttcgcgccgt ctgagcgcgt caccaacgca gcgcccgcct tccacccgtc cgcgtctgag
    17761 cgcgtgacgg actacagggc gccagcgatc tactccgagc cgacttcgac cgtgactgtc
    17821 gtcaagcaca gctatgacgg ccgcacgctc gcgatcgcgc tgtccggagc cgccctgctc
    17881 gtggccttgc tcggcagcgg ctatgtgctg gttcgcctgc acgggttccg gcagaccccc
    17941 gccggcggga tctcctaaaa caacaagccg gcgccatcag gaacgcgggt ctcgcctacg
    18001 ggcggggccc acgtttcgtt tgtaggcaag aacgagtcgc tgtcgtctag gactgcgggc
    18061 ggtagcgcag accgtcgagc gcgatgcgca gcacgtgttc ggtctgacgg gaatcgctgg
    18121 cgggcatcct cgagatgccg ccgaccatgt gcatcacgtc ggagaactcg acgtccggcc
    18181 gtacggcgcc ggcgtcctgg gcgcgtttga gcagcggctc accggcagca aagagcgaag
    18241 cgcgacactc gcggaacagc gcggcgtcct cttcgaggta attcaggagc tcgtgggcca
    18301 gcgcgcgctt cgtgccgaga tagccgatca gccgctcgag ccagacgttg agcgcctccc
    18361 agggatcggc gtcgtcaagt tgcgcggccg agtgagagag cgcctccact tcgttgacgt
    18421 aaagagcctc tagcagcgcc tgccgcgtgg ggaagtgccg gtagagcgtg ccgatgccca
    18481 cgccagcgcg gcgggcgatc tcctcgagag ccgtcgattc tccaccctcg gcgaaggcct
    18541 cgcgcgcagc tgcgagcact cgctcgtagt tgcgcctcgc gtccgcccgc tttgggcgga
    18601 caggcgttgt ctgcacagag gcttggtcgg cgttcatagc aatctacttg acaaacggag
    18661 ccatcctccg tatctttccg gagggttcct ccggatagtt ttggaggcgc cctccatttt
    18721 agcccccgcc ggagacatcg acaaatctcg tgaaccgagc tcctaccgct actcccatat
    18781 ccgccgacca gatgggcctc ggccgggggg ttggctgatg cgacaaggtc cgttggccgg
    18841 ccgctatccc gcggcggccg cgatggtgat cttcgccctg gtgccctacc tcgggctttc
    18901 ggcggcgctg cagccgctga cgccgatcat cggcggtcag ctgcacatga acgcggagac
    18961 gatgagcctc gggttgggca tggcgaacgc tggttacgcg gtcgggacgg ttttggcggt
    19021 gcagttcgcc cagcttctcc cgcagcggcg gatgatggtc ctctacgcag cgttgctcct
    19081 gatcgggtcc gtcttggcgg cggccgcgca ggaccccgcg atgttcattg ccgggcacgt
    19141 gctgcaaggc ctgtgcacca gcctgctgct gatcgcggcc gttccgccat tggcgctcgg
    19201 cttcggcttg gacaagttcc gctcgacggc gatgatcatg aacgtctgca tcttcggtgc
    19261 ggtcgcactt gggcccgtgc ttggcggcat ccaggccggt gctcacgcgt ggcggcctct
    19321 gttctggatc gtcgccggga tctcgctcgc agcgctgctg ctgtcgctat tgacgtttga
    19381 ggacgccccg cccgccgacc cagaagcccc gcgagacctc gtggcgatcg ctcttgctgc
    19441 ttcgggatgc gtggccgcgt tctatggcgc ctcggagctg gtgacgcacc gcttcttggg
    19501 tacccagacg ttcttgccgc tggtcgtcgg cctagcgctg atcgtcgtcc tagtcgtcaa
    19561 ccagtaccga agccggcggc cgctcttgcg ggtccggagc atgctgacga gcacggtgcc
    19621 ggtggccggg atcgtcgtcg ccctggcggc cgcggccgcg tctgtctcgg cgaccgtctt
    19681 gacagcgtca gagctggcgg accgctactc gcccctgcat ctcgggctgc tctatctacc
    19741 ggagttgggt ggcgccttga tcaccgcggc gatgctcggc gtcgtgctca acaaacggca
    19801 gatgcattac ctgccgctcg cgggcatggc gtttctggcg gcggggatcg gagtgtttcg
    19861 tgtccaggtc ccgcccaccg aggccctaac gcttatcggc tccgggctca ccggaatcgg
    19921 tctcggggcg gcggtggccc cagcgctgtt cgtcgccgga ttctcgctgc cgtcggccag
    19981 cctgcaacgg gtgttcgcga tcgtcgagct gttccgcgcg gtcgcagcgt tcatgatcgc
    20041 tccggtcttc gcgcacttcg ccgtcaccgt cggcggcaac gccaggaccg gtaccggcat
    20101 cgcgctgtgg atcggcttcg gtctcgcgat cggcggcgcc atcgcggccg tccttctcta
    20161 cgtggtcggg cgcgcgcgcc cgcagacgcc ggatctcgag cgcttcatgg cgggcgaggc
    20221 gccggcttgg tactcgccgc ccctactagc cggaatccgc aaccgcccgc accatcgagt
    20281 gctcgccgag gacgccgcat gacgcaggac cgcgacaact acccagccgg ccccgtcgtg
    20341 ttcgcctacg acggctccgc actggccagg cttgcgatcg aggaggccgg ccggcagctc
    20401 gcgcccggac gcgacgcgct cgtgctcacc gtctggcaac cattcgacgt cgggttcgtg
    20461 ccacccgtcg agctccgaat cgacgccgcg gagatcgccg acgtcaggaa cgccgccgaa
    20521 caaactgccg ccgagggagc ttcgctggcc gaggcggcgg gcttcaaagc acgcaccacg
    20581 gtggccgaga ccgcgcccag ctggaagggc atcgtccaga tcgccgacga gcacgacgca
    20641 agcctcacag tgctcggctc ccacggccgc accggcctcg ccggcgtact gatcggaagc
    20701 gtcgccgaag cggtcgcagc acactcgcga cgctccgtgc tgatcgtcca ccgccgcgac
    20761 ccacgggatc agaccgaagc tcacgccaac cccgtaaccg agaaagactc agcatgagaa
    20821 cgcccaaccg ctcccgtccc cacaccacga caactgatgc gacccgccgc cgatcacccg
    20881 ggacggagca tgtatccgct tcgagcgcta gcccgcacct gatctacgag gcagtcgtag
    20941 cgagctatct ccacgacatc tcggaacacg accgccagag cgtgccggcc ccgaagaacc
    21001 agaggccgac ctgaagacga ctcggtttcg cagttaccgg acgattccat agccggcgaa
    21061 gcgcctcatg gccgagtgac gccgtggacg gttgatccga tactcgggcg ctgccacgcg
    21121 cgcccggacg tcgcgttgga tgttttttgt ctgctgcatg tccttgcctt tcgtgagtcg
    21181 gttgtcgagt ggtcggtccc cggctgcggc cccgccccgt agggcgaggc cgcacggggg
    21241 tcagtgcgtt cgcagtgcca gcgttgcccg ccgggagctc gaggcgatgc tgcccgagcc
    21301 acgcgtggac gcgacggcca cgaccttcag ccggccgttg cgatccgcca ggcgctggat
    21361 gcccttcggg agcttcacgg tgatgctctt cgtagtgccg cgagcgacgt ggtaacgagc
    21421 actcccgagc tgtagaacgg cgctcactcc cgcgaacctg acgcgcttcg cggtccggag
    21481 cgagagcgac cctgtgcagt tagctgacga gttcgcagag caactcgccc tgaccgacgc
    21541 ccgccccttg ctgactctca gcgtgccgcc cttgatcgtc accgggcgcg tagagcggtc
    21601 gccgggccgg ggcgtggcgt tgggcgtgcg gtcgactgcc tcgaagccgt cgaggatgtc
    21661 gagccgcccg gcatcggcca cgaccgagtc attgcctgcg cctccaactg ccaggtcggc
    21721 cgtcttgtcc cggacgttca cgtgatcgtc accttcatcg cccgagacga agtcgatccc
    21781 gccaccaggc gcgatcgtgt cgttgcccga cccgccgagg aacgtctccg acgagccgcg
    21841 gccggtcagc gtgtcgttgc ccgcgccgcc cgcggcggtc accgcgaagc tgccgacttc
    21901 gccgacggtg atcgtgtcgt cgccggcgag gccgttcgca tgcatcgtct cgctggagcc
    21961 gatgtcgagc gagaacggca cgaggttcgt gcggtcgaac ttgatgcgcg ccccgttggg
    22021 ctggacggtg aagacgtcgc ccgcggttgg agagccgttg acctcgacgt cgtcacggcc
    22081 gtcgtcgccg ttcatcacgt cgctgccgtc gccgttgttc cagaccagcg tgtcgtcgcc
    22141 agcgccgccg ttcatggtgt cgctgccgcg gtcgccgacg atgcgatcgt cgccagcccc
    22201 gccgccgaga acatcgttgc cttcgccgcc gacgaggagg tccggcccgt ccgagccgtt
    22261 gaccgtgtcg tttccggcac cgccgtcgac ggaaagcagc gtgagcgcgc ccacgccgtt
    22321 gctcgctgag acggagtcgt cgccgccgag cccgttgacc tggaagcgct cggtcgaggc
    22381 gttgagcgtg aaggtcacca ggttcgtccg cttgaaggtg atccgccccg cgttgggctc
    22441 gagcgtgaac gtgtcgccga gagtcgggtt gccgttgacc tcggtggtgt cgttgccggc
    22501 gtcgccgtcg atcatgtcgc tgccgtcgcc gttgttccac acgagggtgt cgttgcccgc
    22561 tccgccgctc atatcgtccc cgcccttggc accgacgagg cggtcgttac cgtcgccgcc
    22621 gcccagcgag tcggctgtgt ctgcgccggt cagcaggtcg tcgccggcac cgccgttgag
    22681 ctttgcgccg gcgatctcgt tcgccttggc gagcacggtg agcgagtcgt tgccgtcacc
    22741 gccgttgatg tcgacaccga acgtgccgtt ggcggggacg gtctggtccc cgggcgtcgc
    22801 gctgtcccag tccgagccac tgttgagccc gccgcccgtc tgaccgtgaa cgagtaggcc
    22861 gccggacacc gacaccgtca cgttgtcgtc ggcgccgtcg aggttgagcg tcgccgtctg
    22921 tccatctatc gacgcggagc tgactgccgc gtcggctggg gcggcgttgg tggcgaggcc
    22981 ggccgccagg gctgctccga gcgtggcgag aatcgtcgtg ggtttcatcg tggtcctccg
    23041 gggggtcggg ttgccgtcct aatccaggac cccggcggat gccggtttct gccagagctc
    23101 gctttgcgtc tggaagcagc gggcgtggga tcatcggggc accgtggcgc gcacgctcga
    23161 cccagagtcg ctgggcgacc acctcgaccg gctctttcgg gccgcgtggg cgttatgcgg
    23221 ctctcgcgag gacgccgagg acctcgtcca ggagacctac gagcgggtgc tgcgtcggcc
    23281 gcgcttcgtg cgccgcgacg atgacctcgg atacctgtac cggacgctgc acaacaccta
    23341 cttcaacgcg cggcgcacgg cgtcgcgccg cccgcgggtc accgcgcagc tcgatgacgt
    23401 tcaggcagcg tcctcgcgcg cggacgtcca gcccgagcag gcggcggaga acaacctggt
    23461 gctcgaagcc gtcgcggcgc tgtcccagcc gatgcgggag gtgctcgtcg ccgtggacgt
    23521 gctcgggctc agctacgccg aggccgggaa ggcgctcggc gtccatgaga acaccatccc
    23581 cagccggctc gcccgggctc ggctcgaggt cgccgaggcg ttgcaaggat gaacgcgaag
    23641 cggcagaaaa gcgccccgtg tggggtcatt gggggaagag ggcatggaac ccgaaccgac
    23701 taagcggctc agcgacgacg aactcgccga tgtcgcgcgg ctggtcgacg gcacgctgcc
    23761 ggccgaccgt cgcgccgagg tcgaggcgcg cgtggcggcg tccccgcagc tctcgagcat
    23821 cgtcgagcgc cagcgcgccg ccgtggacgc gctgcgcggc acggccgaga tcggcgcccc
    23881 gcagcagctg cgctcgcgcg tcgaccggcg ccgcggcaag ggacgagcga ctgatcgcgg
    23941 gc
//
LOCUS       DASVXS010000202         8557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_480194, whole genome shotgun sequence.
ACCESSION   DASVXS010000202 DASVXS010000000
VERSION     DASVXS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480194"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VF752_11260"
     CDS             <1..1010
                     /locus_tag="VF752_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX8856170.1"
                     /translation="DRFAELAQIVAVLEREERRALGVTIEGVPVELVVAAPDGFGTAL
                     LRATGAPGYVEALEPLPDAPDEPAVYNALGLPWCPPELREEPFRGEPPPLVEPAEIRG
                     DLHCHTTWSDGRASVEEMGRAARERGYEYLAICDHTPAVGAVRGLTPDDVRRQAEEID
                     AANELLAPFRVLRGIECDILSDGRLDLPDDVLSELDWVQASVHGGQRMPRREMTKRVE
                     EALRNPFVRCLSHPKGRYINRRQENALDLDRVFEVALEEGVALEVNGLPDRLDLRGEH
                     VRDALRAGVQIVCSTDSHSVQGLGNMALSVATARRGWATAANVVNTRPWGTITQRRSA
                     P"
     gene            complement(985..1737)
                     /locus_tag="VF752_11265"
     CDS             complement(985..1737)
                     /locus_tag="VF752_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856171.1"
                     /translation="MAWTETESLSFTARHDHAHQRDAERVLARLESFREELSGLFETT
                     PYDVSVVMHPNALALSLAQPWLPLARRSAAPASRRYFAGWFSTREIHVLAPEALRKRA
                     SNVPGSREALELEPEHEYAHLVVGANNPALPPPFNARTFRSYVRWAWLCEGAATWLSG
                     QTSYLRPAIVRRMRENGRPSFPPDARDAPLLGGTVFDLLERERDVDACVDLATRFDEA
                     AHRRALENAFGMHFTDVERAWREHLTALTAAE"
     gene            complement(1767..5015)
                     /locus_tag="VF752_11270"
     CDS             complement(1767..5015)
                     /locus_tag="VF752_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8856172.1"
                     /translation="MQEPEVLTVLFTDVVGSTELLARLGDDAADSVRRAHFELLRSAI
                     AEHRGREVKSLGDGLMVAFGSARDAVACAVAMQRAVAAERGALELRIGIDAGEPIHEG
                     DDLFGTPVVVARRLCDAAASGQVLVSDLVRLLGGRRLNEPLVPLGSLGLKGLDEPVVA
                     HEVLWRTPAQTPVADDSRAPPPLPLALGRAAEHPFVSRRHELDALHRAWELARGGDGR
                     TVVLAGEPGIGKTSLTAVFAREAYSSGARVLLGRCHPEALVPYEPFVEALRQLPPAAL
                     AKRSRTLARVMPELLAGAPPTAPADDQAERYVLFEAVAHTLAEAARDAPLVLVLEDLH
                     WADQPTLLLLRHSARASERAPLLTLLTFRTTEVPGTEHVMSALTDLERELAFDRITLG
                     GLRDGEVAEMVEEVHGGRASSPLGTALHRETAGNPLFIGQLLRHLEETGALVERDGEL
                     ALTTPGTGLGVPESIKELVGRRLSSLTEETVSTLRTGAVIGRSFPHEVLVWVERNELD
                     ALVDSLEEATAAGLLEESVAGRHAFVHALVREAIYEQTSATRRAGLHRRVAEALEASG
                     GAEPAELAHHFLAARDTGKGLEYSLAAAERSLGQLAYEDAAAHFRRALGALGDEDAGR
                     RCELLIGLADAQARGGDMPAAKVAYRDAAELADNPEHLARAALGYGGRIIWEVSRDDP
                     FMIPLLERAIENLGDADSPLRVRLLVRLAGGPLRDARFPRSRRSDLSREALEMARRLG
                     DPVTLAYAIDGYILAHHGPDQTHEQLRLARELVDVALEGGDKERAMEGFEEQFDARFE
                     LGDVAGAERALASMAELARELRQPSQQWFVAVYRALLALHKGDLREAEHMITAARRLG
                     ETAQAWNAVVSYGLQLYVLRWQQGRIEEVEKLVRRSAEQNPSYHVWDCALAHLAAKLR
                     YHAEASELFGALAEREFAGVPFDEEWLVSMCLLAETANAAGTAQQADVLHGQLLPYAD
                     RVAISYPEISLGAVARYLGIAAAAARRWDEAEYHFRSAHELNGRVKGRPWAAHTQRDH
                     AEMLLARGSGSDRRRARELISSAVAAYSSLGMQHWAERARDLERVGSSAGSA"
     gene            5097..7850
                     /locus_tag="VF752_11275"
     CDS             5097..7850
                     /locus_tag="VF752_11275"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8856173.1"
                     /translation="MRGGRGILAAASCALVALTGASAAQAQDYGGFRNVLAVGQGQTA
                     NAFDLLQYEATGEPPASFNNQRARYTGLVAASPTLAAPQLDDFYKPESFGVSDADTVS
                     TATPNPGVRIARDKYNVPHVFGDTRADVEWGAGYASAQDRLFLMDVLRHTGRANLSEL
                     IGPGTNDSTVKMDAEQLKIADYSESELAKMIDDAERRAGAEGTQVRADLDAYVAGVNG
                     YIAEARTDPAKLPVEYVALGKVPEDWKPTDTVAVASLIGGIFGKGGGSESLAAQALRE
                     AQKRFGSNARSVFDDFRRIDDPEAPTTITDRFPFPSSGKPDPSAVALVDPGSLDTTPK
                     IDKSSGPAAPSASAALPKLPSAEAKAAQEADGWVRSLQDNGLGLHERQSNALLIDAAH
                     STSGRPIAVMGPQVGYYSPEILNELDLHGGGIDARGAAFPGISLYVLLGRGADFAWSA
                     TTATTDNLDQFVEKLCEPDGSTPTRDSDHYLYKGKCIPFVNRDKVLQTTLNPTDPTTP
                     PQTFTIHARRSVHGPIQGTATVDGAPVAIANARSTYFHELDSALAFKRLNDGEVHDAK
                     SFQDAMSKLNFLFNWFYTDDRDIAYLQSGWFPLRAKGTDPSLPTWGTGQWDWQGFDPS
                     TFDSARAGVDALPKAINPKRGYLVSWNNKQAPGWRAADDVWSYGPVQRVQLLQRGMEA
                     AIAGGEKTDLTHLTQIMEEGATEDLRGKEAYPWIRRVIGDRSDPEVSSSLALLDRWQA
                     GGAHRRDANGDNTYDESPAVALMDAWWPRLVNGVYTPELGTPLVDSIRRLIAFDSPPN
                     PGGSSYFDGWWGYIQKDLRTMLGEPVGGGFSRKYCGGGDLAACRTELVSTLKEAAAAV
                     KARYGSVDAAKVPATCDEGQSPPACDQIEFVTAGAVGTKPIPWQNRPTFQQIVEVQGH
                     RPR"
     gene            7861..8265
                     /locus_tag="VF752_11280"
     CDS             7861..8265
                     /locus_tag="VF752_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HEX8856174.1"
                     /translation="MRQQLRPLFDQVVIKELEPDRVRRSGLVVPPGTHEPPPQHGIVL
                     AVGPGLDWWASAGVHMPVKPGDHVVFPASAGTWVDVEEERLLVARVGELLGVLESVKD
                     CPHCSDIVLAAGDTCPVCGRTGQAPAEAFDSD"
BASE COUNT         1333 a         2994 c         3034 g         1196 t
ORIGIN      
        1 tggaccgctt cgccgagctc gcgcagatcg tggcggtgct cgagcgcgag gagcgccgcg
       61 cgctcggcgt cacgatcgag ggcgtcccgg tggagctggt cgtcgccgcg ccggacggct
      121 tcggcaccgc gctgctgcgc gcgaccggcg cacccgggta cgtcgaggcg ctcgagccgc
      181 taccggatgc gcccgacgag ccggcggtct acaacgcgct cggccttccc tggtgtccgc
      241 ccgagctgcg tgaggagccg ttccgcggcg agccgccgcc gctggtggag ccggccgaga
      301 ttcgcggcga tctgcactgc cacaccacct ggtcggacgg aagggcgagc gttgaggaga
      361 tgggccgcgc tgcccgtgag cgcggatacg agtacctcgc gatctgtgac cacacgcccg
      421 cggtcggcgc cgtccgcgga ctcacccccg acgacgtccg ccgccaggcc gaggagatcg
      481 atgccgccaa cgagctgctg gcccctttcc gcgtcttgcg cggaatcgag tgcgacatcc
      541 tcagcgatgg gcggctggat cttcccgacg acgtcctgtc agagctcgac tgggtgcagg
      601 cgagcgtcca cggcggccag cggatgccgc gacgcgagat gacaaagcgc gttgaggagg
      661 cgctacgcaa tccgttcgtg cgttgcctca gccatccgaa ggggcgctac atcaaccggc
      721 ggcaggagaa cgcgctcgat ctcgaccgcg tgttcgaagt ggcgctcgag gaaggagtcg
      781 cgctggaggt gaacggcctg cccgaccgcc tcgaccttcg cggcgagcac gtccgcgacg
      841 cgctgcgcgc cggcgtccag atcgtctgct caaccgactc gcactcggtg caggggctcg
      901 gcaacatggc gctctcggtc gcgaccgcgc gccgcggctg ggcgacggca gccaacgtcg
      961 tgaacacccg gccgtggggc acgattactc agcggcggtc agcgccgtga ggtgctcgcg
     1021 ccatgcgcgc tcgacgtcgg tgaagtgcat gccgaacgcg ttctccagcg cgcgccggtg
     1081 cgcggcctcg tcgaagcggg tggcgaggtc cacgcaggcg tccacgtcgc gctcgcgctc
     1141 gagcaggtcg aagaccgtgc cgccgagcag cggggcgtcg cgcgcgtcgg gcggaaacga
     1201 tgggcggccg ttctcgcgca tgcggcgcac gatcgccggc cgcaggtagc tcgtctgccc
     1261 cgacaaccag gtcgccgcgc cctcgcacag ccacgcccag cgcacatagc tccgaaacgt
     1321 gcgcgcgttg aacggtggcg gcagcgccgg gttgttcgcg cccacgacca gatgggcgta
     1381 ctcgtgctca ggctcgagct cgagcgcctc gcgcgagccc ggtacgttcg acgcccgctt
     1441 acgcagggcc tcgggagcga gcacgtggat ctcgcgagtg gagaaccagc ccgcgaagta
     1501 gcgccggctc gccggcgccg ccgagcgtcg cgcgagcggc agccagggct gagcgagcga
     1561 cagcgcgagc gcgttcggat gcatcacaac gctcacgtcg tacggggtgg tttcgaacag
     1621 ccctgacagc tcttcgcgga agctctcgag cctcgcgagg acgcgttcgg catcccgctg
     1681 gtgagcgtgg tcgtggcgcg ctgtgaacga cagcgactcc gtctccgtcc aggccatggg
     1741 gccgagcagt ctgccaaggc ggcgcgctac gcggagccgg cggaagaccc aactctctcg
     1801 agatcccgag cgcgctcggc ccaatgctgc ataccgagcg acgagtacgc ggcgaccgcg
     1861 ctggagatca gctcgcgcgc gcgacgccgg tcggagccag agccccgcgc gagcaacatc
     1921 tcggcgtgat cgcgctgagt gtgagcagcc cacggacgcc ccttcacgcg gccgttcaac
     1981 tcgtgcgccg accggaagtg gtactcggcc tcgtcccagc ggcgcgccgc cgctgcggcg
     2041 atcccgaggt agcgcgccac cgcaccgagg cttatctcgg gatagctgat tgcgacgcgg
     2101 tcggcgtacg gcagcagctg cccgtgcagc acgtccgcct gctgggcggt gcccgcggcg
     2161 ttcgccgtct cggcgaggag gcacatgctc accagccact cctcgtcgaa gggcacgccg
     2221 gcgaactcgc gctcggccag tgcgccgaac aactcgctcg cttccgcgtg gtaacggagc
     2281 ttcgcggcga ggtgtgcgag ggcgcagtcc catacgtggt acgacgggtt ctgctccgcc
     2341 gagcgccgga ccagcttctc cacctcctcg atgcggccct gctgccagcg cagcacgtag
     2401 agctgaaggc cgtacgacac aacggcgttc catgcctggg ccgtctcgcc caatctccgc
     2461 gctgcggtaa tcatgtgctc cgcctcgcgc aaatccccct tgtgcaaggc cagcaacgcg
     2521 cggtagaccg cgacgaacca ctgctgcgac ggttggcgca gctcccgcgc cagttcggcc
     2581 atgctcgcga gcgcgcgttc ggctccggcg acatcaccga gctcgaagcg ggcgtcgaac
     2641 tgctcctcga agccctccat cgcccgttcc ttgtctccac cctcgagcgc gacgtccacc
     2701 agttcgcgcg cgagcctgag ctgctcgtgg gtctggtcgg gcccgtggtg ggcgaggatg
     2761 tagccatcga tcgcgtacgc gagcgtgact gggtcgccaa gccggcgcgc catctcaagc
     2821 gcctcgcgac tcaggtctga gcgcctgctc cgcgggaagc gagcgtcgcg gaggggaccg
     2881 cccgcaagcc ggacgagcag gcgcacgcgc agagggctgt cggcgtcccc gagattctcg
     2941 atcgcccgct cgagcagagg gatcatgaac gggtcgtccc tcgacacttc ccaaatgatc
     3001 cggccgccgt atccgagggc ggcgcgtgcg aggtgctcgg gattgtcggc caactcggcg
     3061 gcgtcgcggt aggcgacctt cgcggcgggc atgtctccgc ctctcgcctg ggcgtcggcc
     3121 aggccgatca gcagctcgca gcggcggccc gcgtcctcgt cgccgagcgc tccgagtgcg
     3181 cgacggaagt gcgccgcggc gtcctcgtac gcgagctgac cgagcgaacg ctccgctgcg
     3241 gcaagcgagt actccaggcc ctttccggtg tcgcgcgccg cgaggaagtg atgggcgagc
     3301 tcggccggct cggcaccgcc cgaggcctcg agtgcctcgg ccacgcggcg gtgcagaccc
     3361 gcgcgcctgg tcgcacttgt ctgctcgtag atggcctcgc gcacgagcgc atgcacgaac
     3421 gcatggcggc cggcgaccga ctcttcgagc agccctgcag cggttgcctc ctccagactg
     3481 tcaaccagcg cgtccaactc attccgctcg acccacacga gaacctcgtg tggaaatgag
     3541 cggccgatca cggcgccggt gcggagcgtc gagaccgtct cttcggtgag actggagagc
     3601 cgccggccca caagctcctt gatgctctca gggacgccca gccccgttcc cggcgtggtg
     3661 agcgccagct cgccgtcgcg ctccacaagc gcgccggtct cctcgagatg ccgcaggagc
     3721 tgtccgatga agagcgggtt ccccgccgtc tccctatgca gcgcggtgcc gagcggcgac
     3781 gaggcgcgcc cgccgtggac ctcctcgacc atctcagcca cctcgccgtc gcgcagaccg
     3841 ccgagcgtga tgcgatcgaa cgcgagctct cgttcgaggt cggtgagcgc gctcatcacg
     3901 tgctcggtgc cggggacctc tgtggtcctg aaggtgagca gcgtgagcag cggcgcccgc
     3961 tcggaagcgc gcgctgagtg gcgaaggagg agcagcgtgg gctgatcggc ccaatgcagg
     4021 tcctcgagca ccaggacgag cggtgcatcg cgggcggcct ctgcgagcgt gtgcgcgacc
     4081 gcctcgaaga gcacataccg ctcggcctga tcgtcggcgg gcgcggtagg cggcgcgccg
     4141 gccagcagct cgggcatcac gcgggcaagg gtgcgagagc gcttcgcgag cgcggcgggc
     4201 ggcagctgcc gaagcgcctc cacgaacggc tcgtacggca cgagtgcctc gggatggcag
     4261 cgtccgagca ggacgcgtgc tccggacgag tatgcctccc gcgcgaaaac ggcagtcagg
     4321 ctcgtcttgc cgatgccagg ctccccagcg agcaccacgg tgcggccgtc tccgccacgg
     4381 gcgagctccc aagcccggtg aagggcatcg agctcgtgcc ttctcgacac gaagggatgc
     4441 tccgccgcgc gcccaagcgc gagcggcaac ggtgggggtg cgcggctgtc gtccgcaacg
     4501 ggtgtctgcg ccggagtccg ccacagcact tcgtgtgcga caaccggctc gtcgaggccc
     4561 ttcaacccca gcgagccgag cggcacgagg ggctcgttca ggcgccgccc gccgagtagt
     4621 cgcacaaggt ccgagacgag cacctggcca ctggccgccg cgtcgcagag acgccgcgcc
     4681 acgaccaccg gcgttccgaa caggtcgtcc ccctcatgga tcggctctcc ggcgtcgatg
     4741 ccgatccgca gctcgagtgc tcctcgctcg gcggcgaccg cccgctgcat cgcgacagcg
     4801 catgccacgg cgtcacgcgc gctgccgaac gcgaccatga gcccgtcgcc gaggctcttc
     4861 acctcgcggc cgcgatgctc ggcgatcgcc gaacgcagca gctcgaaatg cgcacggcgg
     4921 acggagtcgg ccgcgtcgtc gcccagccgg gccaacagct cggtggagcc gacgacatcg
     4981 gtgaacagga ccgtgagtac ttcaggctcc tgcacaacgg cgatcctaaa tccgccgctc
     5041 gagcggagct agggaactgg agcgcgcacc ggcgacgact tgtaatgtgc cgcgccatgc
     5101 gaggtggcag ggggatcctc gccgcggcgt cgtgcgcgct cgtcgcgctc acgggcgcgt
     5161 cggcggctca ggcgcaggac tacggcggct tcaggaacgt gcttgcggtc ggccaggggc
     5221 agaccgccaa cgcgttcgac ctacttcagt acgaggcgac gggcgagccg cccgcgagct
     5281 tcaacaacca gcgcgcccgg tacaccggac tcgtggcggc gtcgcccacg ctcgcggcgc
     5341 cacagctcga cgacttctac aagccggagt cgttcggcgt gtccgacgcg gacacggtct
     5401 cgactgcgac gccgaatccc ggcgtacgca tcgcgcgcga caagtacaac gtgccgcacg
     5461 tgttcggcga cacgcgcgcc gacgtcgagt ggggcgccgg gtacgcgagt gcgcaggacc
     5521 ggctgttcct gatggacgtc ctgcggcaca ccggccgcgc gaacctcagc gagctgatcg
     5581 ggcccggcac caacgactcg acggtgaaga tggacgccga gcagctcaag atcgccgatt
     5641 acagcgagtc ggagctggcc aagatgatcg acgacgccga gcggcgcgcc ggggcggagg
     5701 gcactcaggt gcgtgcggac ctcgacgcgt acgtggcggg ggtgaacgga tacatcgccg
     5761 aggcgcgcac ggatcccgcg aagctgccgg tggagtacgt cgcgctcggc aaggtcccgg
     5821 aggattggaa gccgactgac acggtcgcgg tggcgtcgct catcggcggc atcttcggca
     5881 agggcggcgg ctcggagtcg cttgccgcgc aggcgctgcg ggaggcgcag aagcgcttcg
     5941 ggtcgaacgc gcgctcggtg ttcgacgact tccggcgcat cgacgatccc gaggcgccga
     6001 ccacgatcac cgaccgcttt ccgttcccgt cgagcggcaa gccggacccg agcgccgtcg
     6061 cgctggtcga tccgggctcg ctcgacacca cgccgaagat cgacaagtcg agcggaccgg
     6121 cagcaccgag cgcgagcgcg gcgcttccga agctcccttc ggccgaggct aaggccgcgc
     6181 aggaggcaga cggctgggtg cgctcgctgc aggacaacgg cctcggcctt cacgagcgcc
     6241 agtcgaacgc gctgctgatc gatgccgcgc actccacctc aggccgtccg atcgcagtga
     6301 tgggcccgca ggtgggctac tactcgcccg agatcctcaa cgagctcgac ctgcacggcg
     6361 ggggcatcga cgcgcgcggc gcggcgttcc cgggcatcag cctgtacgtg ctcctcgggc
     6421 gcggcgccga cttcgcgtgg agcgcaacca cggctacgac ggacaacctg gaccagttcg
     6481 tggagaagct ctgcgagccg gatggctcga cgccgacgcg cgactcggac cactacctct
     6541 acaagggcaa gtgcattccg ttcgtgaacc gggacaaggt cctgcagact acgctcaacc
     6601 cgacggatcc cacaacgccg ccgcagacgt tcacgatcca cgcgcggcgc agcgtgcacg
     6661 ggccgatcca gggcacggcg acggtcgacg gcgcgcccgt cgcgatcgcg aacgcgcgct
     6721 cgacgtactt ccacgagctc gactcagctc tcgccttcaa gcgcctgaac gacggcgagg
     6781 tgcacgacgc gaagtcgttc caggacgcga tgagcaagct gaacttcctg ttcaactggt
     6841 tctacaccga cgaccgggac atcgcgtacc tgcaatcggg ctggttcccg ctgcgcgcga
     6901 agggcacgga cccgtcgctg ccgacctggg gcactgggca gtgggactgg cagggcttcg
     6961 acccgagcac cttcgactcc gcacgggcgg gcgttgacgc gctacccaag gcgatcaacc
     7021 cgaagcgtgg ctacctcgtg tcgtggaaca acaagcaggc acccggctgg cgcgcggccg
     7081 acgacgtgtg gtcgtacggc ccggtgcagc gcgtgcagct gctccagcgc ggcatggagg
     7141 cggcgatcgc gggcggcgag aagaccgatc tgacgcacct cacgcagatc atggaggagg
     7201 gggcgacgga ggacctgcgc ggcaaggagg cctacccgtg gatccggcgg gtgatcggag
     7261 acaggagcga ccccgaggtg agctcgtcgc tcgcgctgct cgaccgctgg caggccggcg
     7321 gcgcgcacag gcgtgacgcc aacggcgaca acacctatga cgagtcgccc gccgtcgcgc
     7381 tcatggacgc ctggtggccg cggctcgtga acggcgtcta cacgcccgag ctcggcacgc
     7441 cgctcgtcga ttcgatcagg aggctgatcg cgtttgactc gccgccgaac ccgggcggca
     7501 gctcgtactt cgacggctgg tggggctaca tccagaagga cctgcgcacg atgctcggcg
     7561 agccggtggg cggcggcttc tcgcgcaagt actgcggcgg gggcgacctc gcggcgtgtc
     7621 gtacggagct ggtctccacg ctgaaggagg ccgccgcggc ggtgaaggcg cgctacggca
     7681 gcgtcgacgc cgccaaggtt cccgccacct gcgacgaggg acagagcccg cccgcctgcg
     7741 accagatcga gttcgtgacc gctggcgccg tcggcaccaa gccgatcccg tggcagaacc
     7801 ggcccacttt ccagcaaatc gtcgaggtcc agggccaccg accgcgctag attcagggct
     7861 gtgaggcagc agcttcggcc ccttttcgac caagtcgtga taaaggagct cgagccggac
     7921 cgcgtccggc gctcgggtct tgtcgtgccg cccggcaccc atgagccgcc gccgcagcac
     7981 gggatcgtgc tggcggtcgg tccgggcctc gactggtggg ccagcgccgg agtgcacatg
     8041 ccggtgaagc ccggcgacca cgtggtgttc ccggcgtcgg ccggcacatg ggtcgacgtg
     8101 gaggaggagc ggctgctcgt cgctcgcgtg ggcgagctgc tcggcgtgct cgagtccgtg
     8161 aaggactgcc cgcactgctc cgacatcgtg ctcgcggcgg gcgacacctg ccccgtatgc
     8221 gggcgaacgg ggcaggcgcc cgccgaagcg ttcgattcgg actagcgctt ccgcggttcg
     8281 gtagagcagc aaaggacgca ggagcgggcg gtttttctgt accgtcctgg attgcccccc
     8341 atatgcgggg gtaaagcagg acgctttgcg atttttctaa cgagccgagg gacgtagcgc
     8401 ggcggggaag cgggcgccgc cggggcggcg gcccctgtgc ccggtgccta ggacgcgaac
     8461 tcgctcgtcg ctccggagta gcgaagcgag cccggcgtgg tgagcagctc gccggtctcg
     8521 agcagcgtct tgaggccgga gaggatcatc ggccagc
//
LOCUS       DASVXS010000203        15419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_805555, whole genome shotgun sequence.
ACCESSION   DASVXS010000203 DASVXS010000000
VERSION     DASVXS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805555"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VF752_11285"
     CDS             complement(<1..1710)
                     /locus_tag="VF752_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX8856175.1"
                     /translation="MSSQLSSNEVLLGLGLVLVLAVAAQLLASRLHLPAIVALLPIGF
                     IAGAATDDVHPDALLGQLYQPFVSIAVGVILFEAGLRLSFADIAPGVRRAVVRLILVG
                     VPVTWLAVTGAVALLFNDVSTGVAFLIGAILVVSGPTVVLPLLAFIRPSGNVRSLLRW
                     EGVLVDPVGALLGVLVFHAVRSGGPHGSGWQPGSMLASVAVGALVGAAGAAVLWVLLR
                     GIQRTAPRQAVPATLMVVVAALVGADLIRDDSGFVATTLMGVFLANQRRISVALTAAF
                     QETLVQLLVGVLFILIAASVSPSEVKAVLPEGILLVALMVLVIRPAAVALATWRSILS
                     VRERLFAAWMAPRGIVAGATASAFGLQLAQEGIKGADQVLPIVFVAIFGTVVVYGLTG
                     PLVARLLGLAGEEGTLMLVVGGHDWARELADALRKAGIRIRLWAGPEEYRAAAREAGL
                     DADRGRMMIDVVNREAELEEVTDALVLSGSDDFNSLVAADLRDELGHEHVYRVAPDPE
                     APDLLPPPEETGILADTHLTFAEMSRRFAQGARVVSETADSRPDGPTGSDSIPLFVVT
                     PDGE"
     gene            1870..3309
                     /gene="zwf"
                     /locus_tag="VF752_11290"
     CDS             1870..3309
                     /gene="zwf"
                     /locus_tag="VF752_11290"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX8856176.1"
                     /translation="MATAVEPRARSKAAGVARPTAPADVFVAFGITGDLAKQMTFRSL
                     YRLERRGLLDCPIVGVAVDEWSDDELRQHARSAIEAGGETLDPDVFERFAARLSYLSG
                     NFGDPATFERLARAIGDARSPVFYLEIPPFLFGRVIKGLSDAGLTKSARVVVEKPFGH
                     DLASARELNDEVHQYIDESQLYRIDHFLGKMGLVEILYLRFANRMFEPLWNRSHVSSV
                     QITMAESFGVEDRGHFYDPVGALRDVVVNHMMQLVGAAAMEPPAGGDPATLKDALHGV
                     FRAMPDADPAHYVRGQYDGYRDIDGVATDSTTETYAAMRLDVDNWRWSGVPFFLRTGK
                     RMPTTQTEMRIVLQRPPRLGFHIVKQPPEPSQVVVKLDPSTGIRLLLEAQRSGTREPE
                     QISLDMEFSEQGGEGAAPYEVLLHAAMLGDSTRFTRQDGVEEAWRVMQPLLDQPPPVH
                     PYKQGSWGPEAANHLLADHGRWYGPWMTS"
     gene            3306..4673
                     /locus_tag="VF752_11295"
     CDS             3306..4673
                     /locus_tag="VF752_11295"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8856177.1"
                     /translation="MSGQQGRHRVVIIGGGFGGLFAAKFLRRAPVDVVLLDRMNHHLF
                     QPLLYQCATGILSEGAIAPPLRQVLRKHENVSVELANVTDIDLERRRVIAERVMGSPV
                     EFDYDSLIVATGAASSYFGRDEYSRFAPGLKTIDDALEVRARIFGAFEMAELEEDPEA
                     RRAWLTFAVIGAGPTGVEVAGQIIELAHRSLKGNFRRIDPASARVLLFDAGDAILAGF
                     GNKLSTVAERSMKRLGVDVRTGSRVTEITYRDITVEGSSGPETIPCHVKVWAAGVRAS
                     PLAAMLAEASGAGTDRAGRIEVLPDCSLPGHPEVFAVGDMMALDKLPGVAEVAMQSGI
                     HAAETIKRRVTGGGDSKPFKYRDLGSMASIARFSAIVDFKGIRMSGFIAWLMWVFVHL
                     AFLTGFKNRFIALSKWLTAFIGESRDERTGSALQASARVIVAAAGVKPGEQDMTRLAE
                     REHRP"
     gene            complement(4691..6307)
                     /locus_tag="VF752_11300"
     CDS             complement(4691..6307)
                     /locus_tag="VF752_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8856178.1"
                     /translation="MSAVVSKPTTGAPNRAGLVLASLILVAAVANLNLSVANVALPDI
                     GRHFDSSQTMLDLIAVGYSLGLACSVLWLGALGDRYGRKSMLLGGTVLAIPMSLLAAF
                     APSDTVLFIARFGGGLAAGMAYPTTLSLITALWAPEAGRTKAIALWSGLGGAIAALGP
                     VLAGLLLEHFDWGSVFVITLPLALVSIYMAWRYVPAHVNEGSEAVDNLGGIVSVLLVA
                     ALILSINFAPVDGKGTQSLILGGVAAIAVVVFYLRERKARNPLYDLKVAARPPFWVAA
                     TAGIIVFGSLMGAMFIGQQFLQNVLGYSALGAGASILPAALFLVLVAPRSAKLVEAHG
                     ARFTLLCGYVFVLLGFLAMLLLWKENIDWWKVGLGYALVGIGVGLAGTPASHSLTGSV
                     PVKRVGMASGTADLQRDLGGAVMQSIFGALLTAGYATSAGSQIAASGKNITSSVQNSL
                     TKSFDGAEAIAKQYPQYADKITAGAKTAFLQGDQWAYLAGIIAVLLGALLVFFVFPKK
                     DREQQLLAEYRDQDRIESPAPEPAEPVVAA"
     gene            6406..7800
                     /locus_tag="VF752_11305"
     CDS             6406..7800
                     /locus_tag="VF752_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX8856179.1"
                     /translation="MLPFASTTVDLARIQFATTSIYHFLFVPLTLGLAPLVAVMQTIW
                     HRTHDEAWLRLTRFFGTLLLINFAIGVATGLVQEFQFGMNWSVYSNFVGGVFGAPLAI
                     EGLAAFFLEATFLGLWIFGWGRLSPRIHLATIWLAALGTWLSAYFILVANSWMQHPVG
                     YKVVNGKAELTDVGALLSNSFALRAYLHVLLVGLTTASIVMLGICCWHLLRGRNVELF
                     RRAAKLALIVALPITLINLIVGNRFGEAVTSAQAMKIASAEALWNTEQPAGFSLFQVG
                     GFSSQDPTPSVSVTVPRLLSYMATGSFNGKVEGINQLQAQESQRFGPGNYIPDVRVIY
                     WSIRVMAYLGVLMFLVAAVGAWLYRKRRLEKARWFLWSAVVAIAFPFIAAIAGWLLTE
                     MGRQPWIVQGLLKTSEANSPNVSTATIGTSLGLFVLLYITLGIVDFVLMRRYARVDPP
                     EIEAPAPAGAALGY"
     gene            7811..8818
                     /gene="cydB"
                     /locus_tag="VF752_11310"
     CDS             7811..8818
                     /gene="cydB"
                     /locus_tag="VF752_11310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX8856180.1"
                     /translation="MGLEVFWFCLIGVLWGGYFLLEGFDFGVGMLLPLLGRKESDRKT
                     MFETIGPVWDGNEVWLVVAGAATFAAFPVWYATMFSGFYLALVIVLLLLIVRVVSFEW
                     RERSENPRWRASWQWANTVGSIGAPFVWGVALANLIYGVPLNGDGGYAGDFLDLFSAY
                     TVFAGVVVVFLFALHGATFLTIRATGSLREHAGTLARRLAIPVAVLAAGLLAWTVAVA
                     HDHNKRGVLPPAIPAAITLGTLLLAVAFVLARRHGWAFAMTALAAVGVVATIFTGLYP
                     RVLVSDPTFANSLTISNAASSHYALKVMTIVAAIFTPLVLLYQAWTYYVLRRRLGGAT
                     G"
     gene            8828..10504
                     /gene="cydD"
                     /locus_tag="VF752_11315"
     CDS             8828..10504
                     /gene="cydD"
                     /locus_tag="VF752_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEX8856181.1"
                     /translation="MRTLDQRLLRRTRATRPLLVLDTALGIATVVPVIAQATLLAHIV
                     TRAFDGASLPALRLDLGLLLLAFAARGALAWSMEVAGRRAASSVLSELRLELAARRLT
                     HHPAAEDGVEAGEIAAAAVQGVDALEAYFARYLPQVVLACVVPIAVVGWVASIDPLSA
                     LIMLLTLPLVPVFMWLIGRYTEQRTRERWDALRSLSTHFLDVVRGLPTLRAFNRAVAQ
                     ADSIADVSERYRKATMGTLRVSFLSGSVLELAATLGVALVAVTIGVRLVDGGLGLAAG
                     LTVLVLAPELYLPFRRLGAEYHASADGLAVAERMFGLLGAPPAVRAVGAVAPYNPAEF
                     PVRFESVCFSYPTRPGRVVDGLDMELAPGETVALVGESGCGKSTVAALLLRLIEPTGG
                     RITVGGVDLAVCSTDAWRRLVAWVPQHPSLLRGTVTENIRLGVPGASIDRVREAARLA
                     GADGFIEALPEGYDTIIGDGGRRLSPGERRRVGLARAFLRDAALVILDEPTADLDPES
                     VERVADAVERLRDGRTMLLIAHRPELVGRADRVVLLESGAAVPLRMREAA"
     gene            10501..12138
                     /gene="cydC"
                     /locus_tag="VF752_11320"
     CDS             10501..12138
                     /gene="cydC"
                     /locus_tag="VF752_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEX8856182.1"
                     /translation="MTATLRRLLVLADAPRARVGLTVVLGALTVLFGAGLMGTAGYLI
                     SRAAERPAILSLTVAIVAVRFFGIGRPVARYLERITSHDLALRVLGRVRVRVFRRIEP
                     LAPAQLEGYRSGDLLSRMVADVDSLQNLHLCGVSPPLVAALAGVVSVGVAAAFLPAAA
                     LVLGCGLLVGAVAVPAVAGWLGSRAARRQAAVRGHLSAELVELLDAAPELVVHGAGEA
                     ALARLGGVDRSLVRLARRDAAAGGVADGLCLFITGVTVAGVLATAVHASASGHLGRVL
                     IAMLALLALASFEAIQPLSAAARELGATLAAGRRLLELVDRAPAVVDPQQPLPAPRAP
                     FAVALEEVRARYSPRERPALNGFSLHLEPGRRVALVGPSGAGKTTVVNLLLRFLDPEA
                     GRVTLAGRDLRDYRQEDVRRAIAVAGQESHLFSASIRENVRLARPAASDAEVEDALRH
                     ARIWDWVDTLPDGWDTRVGETGRELSGGQAQRVVLARALLADAPVLVLDEPTAHLDPA
                     TATELVSDIFAAGVGHSILLITHRPEGLDLVDEIVTL"
     gene            12284..12610
                     /locus_tag="VF752_11325"
     CDS             12284..12610
                     /locus_tag="VF752_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856183.1"
                     /translation="MPGEIVASVMHLPHVTDRGDEELGLLLRWGYGSAFGLLHGLLRR
                     RVGEPWAAGAFGGTLMTATLTLFPLLGRTPPPWRWGADVVATCVGTHFAYAASVATAD
                     NAMRRR"
     gene            12617..12859
                     /locus_tag="VF752_11330"
     CDS             12617..12859
                     /locus_tag="VF752_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEX8856184.1"
                     /translation="MLSTDDPHSFETRASAAVGVAISVTTIPASAYLGVGVGETSKAL
                     GALLVLGINVSMLTLGGSLTLLLQRWLARHEGLSPR"
     gene            complement(12956..13016)
                     /locus_tag="VF752_11335"
     tRNA            complement(12956..13016)
                     /locus_tag="VF752_11335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12971..12973),aa:Ser,seq:cga)
     gene            complement(13200..13273)
                     /locus_tag="VF752_11340"
     tRNA            complement(13200..13273)
                     /locus_tag="VF752_11340"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13237..13239),aa:Met,seq:cat)
     gene            13524..14987
                     /locus_tag="VF752_11345"
     CDS             13524..14987
                     /locus_tag="VF752_11345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8856185.1"
                     /translation="MTRASGSSPFAPGCNVSTLGTVYPNSEVEPYVASNPVDTNDLVG
                     VWQQDRWSSAAAQGQLTAVSSDRGSTWTLPAPPPFSHCQGGNATNGGDYDRTSDPWVT
                     FGPTGDSYQSSLAVSNDQVTSAILVSKSSDHGQTWGPIATLRRDDDPHFLNDKESITA
                     DSLAPNRAYAVWDRLFALDPTNPNSDFFGPIWFARTTNGGATWRRAHKIFDPGLDNQT
                     IGNQIVELPDGELLDVFDLIIQGNLNVALIRSPDQGRTWSQPTIVDQEASIGVVDPMD
                     AAPVRTGDIIPEVAVDPRPGTNNVYLVWQDARFSAGHADQVVFTSSSDGGRTWTPVTR
                     ISQDGILQAFTPMITVAGNGDIAVTYYDFSFDTPASPTLDTDYWVTRSTDGGATFQPR
                     ERITPTSFDMRTAPVARGFFVGDYEGLAPSAQFSPFFAAANSGDLSNPTDVFSTLVHA
                     PFPTAAATVRRPRAHRAKFARRSPGHIAASRRPVRVR"
     gene            complement(14984..15419)
                     /locus_tag="VF752_11350"
                     /pseudo
     CDS             complement(14984..15419)
                     /locus_tag="VF752_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263657636.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
BASE COUNT         2335 a         5296 c         5254 g         2534 t
ORIGIN      
        1 ctctccgtct ggggtgacga cgaacaacgg gatggagtcc gacccagtcg gaccgtcggg
       61 gcgcgagtcc gccgtctcgc tcaccacacg tgcaccttgc gcgaagcgcc gactcatctc
      121 cgcgaaggtg aggtgagtat ccgcaaggat gcccgtctcc tcgggcggcg gcagcagatc
      181 gggcgcttcg gggtcgggag cgacccggta cacgtgctca tggcccagct cgtcgcggag
      241 gtcggccgcc accagagagt tgaagtcgtc gctcccactc agtacgagcg cgtcggtgac
      301 ctcctcgagc tccgcctcgc gattcaccac gtcgatcatc atccggcccc gatccgcgtc
      361 cagcccggcc tcgcgcgcag ccgctcggta ctcctccgga cccgcccaga ggcggatccg
      421 gatcccggcc tttctcaggg cgtcggcaag ctcgcgtgcc cagtcgtgtc cgcccacgac
      481 aagcatgagg gtgccctctt cgccggccag tccgaggagg cgagccacga gcggtccggt
      541 gagtccgtag accacgacag tgccaaagat cgcgacgaac acgatcggaa gcacctgatc
      601 cgcgcccttg atcccctctt gcgcgagctg tagaccgaag gccgaggccg tcgcgcccgc
      661 cacgatcccg cgtggcgcca tccaggcagc gaacaggcgc tcacggacgc tgaggattga
      721 tcgccaggta gcgagcgcga ccgccgcagg ccggattacg agcaccatca gcgcgaccag
      781 gagaatcccc tccggaagga ccgccttgac ttcgctcggg gagaccgagg cggcgatcag
      841 gatgaacaag acgcccacca gcagctggac cagggtctct tggaacgccg ccgtcagagc
      901 gacgctgatg cggcgctggt tcgcgaggaa gacacccatc agcgttgtcg ccacaaagcc
      961 cgagtcgtcg cgaatcaggt ccgcgcccac gagcgcggcc accacgacca tgagcgtggc
     1021 cggcaccgcc tgccgcggtg ccgtccgctg aatgccacgc agcagtaccc aaaggacggc
     1081 cgcccccgcg gccccgacga gcgcgccgac ggcaacgctc gccagcatcg agcccggctg
     1141 ccagccagac ccgtgaggtc cgccggagcg gacggcatgg aagacgagca ctcccagtag
     1201 cgctccgacc gggtccacca gcacgccctc ccagcgcagc agcgagcgta cgtttccgct
     1261 cggccggatg aacgcgagca ggggaagcac gacggtcggc cccgagacca ccaggatcgc
     1321 gccgatcagg aacgcgacgc cggtcgagac gtcgttgaac agcagggcga cggcgcccgt
     1381 gacagcgagc catgtgaccg ggaccccgac gaggatcagc cggacgacgg cgcggcgtac
     1441 gccgggcgca atgtcggcga acgagagtct cagccccgcc tcgaacagga tcacgccgac
     1501 cgcgatcgag acaaagggct ggtagagctg gccgaggagc gcgtctgggt ggacgtcgtc
     1561 cgtcgccgcc ccggcgatga agccgatcgg caggagcgcc acgatcgccg ggaggtgcag
     1621 cctgctcgcg agtagctgag ccgccacggc gaggaccagc accagcccca gacccagcag
     1681 tacctcgttg ctcgacagct gcgacgacac ggcgaggacg atcgtcccgc cgagcgtgcg
     1741 cggcgttcat caccccgatt aggtgaggcg gccaccgcgg cggaagttca cccggatcag
     1801 atgatgatcg cgttcgcctc ggcgcggagc atcggctcgc actcgcgaca ctccggagga
     1861 ggaagaaaca tggccaccgc agtcgagccc cgagcacgct cgaaagcagc cggagtcgct
     1921 cgccctacgg cgcccgcgga tgtgttcgtc gcgttcggaa tcaccggcga cctcgccaag
     1981 cagatgacgt ttcgatcgct ctacaggctc gagcgacgcg gattgctcga ctgcccgatc
     2041 gtgggcgtcg ccgtggacga gtggagcgac gatgagctcc gtcagcacgc tcgcagcgca
     2101 atcgaagcgg gcggcgagac gctcgacccg gacgtcttcg agcgcttcgc cgcgaggctg
     2161 tcgtacctaa gcggcaattt tggcgaccct gcgaccttcg agcgtctggc gagggcgatc
     2221 ggcgatgccc gaagccccgt cttctacctc gagataccac cgttcctgtt cgggagggtg
     2281 atcaagggcc tgtcggacgc cggactgacg aagtccgccc gcgtggtcgt ggagaagccg
     2341 ttcgggcacg acctcgcatc ggctcgcgag ctcaacgacg aggtgcacca gtacatcgac
     2401 gagtcgcagc tctatcggat cgaccacttc ctgggaaaga tggggctcgt cgagatcctg
     2461 tacctgcggt ttgcgaaccg catgttcgag ccgctctgga accgcagcca cgtgtcgtcc
     2521 gtgcagatca cgatggccga gagcttcgga gtagaggacc gcgggcactt ctacgacccg
     2581 gtgggcgccc ttcgcgatgt ggtggtcaac cacatgatgc agctggtcgg cgccgccgcg
     2641 atggagccgc cggcgggcgg cgaccccgca accttgaagg acgcgctgca tggcgtcttt
     2701 cgcgcgatgc cagacgccga tccggctcac tacgtacgcg ggcagtacga cggctaccgc
     2761 gacatcgacg gcgttgcgac ggattcgacc accgagacct acgcggcgat gcgcctcgac
     2821 gtcgacaact ggcgctggag cggcgtcccg ttcttcctcc gcacgggcaa gcgcatgccc
     2881 accacgcaga cggagatgcg gatcgtgctc cagcgtccgc cgcggctcgg cttccacatc
     2941 gtcaagcagc cgcccgagcc gagccaggtg gtggtgaagc ttgatccgtc gaccggcatc
     3001 cggctgctct tggaggcgca gcgcagcggc acccgtgagc cggagcagat cagcctcgac
     3061 atggagttca gcgagcaggg aggcgaggga gccgcaccct acgaggtgct cctccacgcg
     3121 gcgatgctgg gagacagcac acgattcacg cggcaggacg gtgtcgagga ggcctggcga
     3181 gtgatgcaac ctctgctcga tcaacctccg ccggttcacc cgtacaagca gggatcgtgg
     3241 ggtccggagg ccgcgaatca tctcctggcc gaccatggcc gctggtacgg cccgtggatg
     3301 acgtcatgag cgggcagcag ggccggcacc gcgtcgtgat catcggcggc ggcttcggag
     3361 gcttgttcgc cgccaagttc ctcagacgcg cgccggtcga cgtggtgttg ctggaccgga
     3421 tgaaccacca cctgttccag ccgcttctct accagtgcgc gacgggcatc ctgtcggaag
     3481 gggcgatcgc gccgccgctg cggcaggtgc ttcgcaagca cgagaacgtt tcggtcgagc
     3541 tcgcgaacgt gaccgacatc gacctcgagc ggcggcgcgt gatcgcggag cgggtcatgg
     3601 ggtcgcctgt ggagttcgat tacgactcgc tgatcgtcgc gaccggcgcc gccagctcct
     3661 acttcggtcg cgacgagtac tcgcgcttcg cgcccggcct gaagacgatc gacgacgcgc
     3721 tggaggtgcg agcccggatc ttcggcgcgt tcgagatggc cgagctcgag gaagatccgg
     3781 aggcgaggcg ggcgtggctg accttcgccg tcatcggcgc cggtcccaca ggtgtcgagg
     3841 ttgcgggcca gatcatcgag ctcgcgcacc gttcgctgaa gggcaatttc cggcgtatcg
     3901 acccggccag cgcgcgcgtc ctgctgttcg acgccggcga cgcaatcctc gccggcttcg
     3961 gcaacaagct ctcgacggtc gcggaacggt cgatgaagcg actcggcgtg gacgtgcgga
     4021 ccggttcacg cgttaccgag atcacctatc gggacatcac ggtcgagggc tcgagcgggc
     4081 cggagacgat cccgtgccat gtgaaggtgt gggccgccgg cgtgcgggcg tctccgttgg
     4141 cagcgatgct ggccgaggcg agcggagccg gcaccgaccg ggcaggacgc atcgaggtgc
     4201 tccccgattg ctcgctgccc ggccatcccg aggtgttcgc tgtcggcgac atgatggcgc
     4261 tggacaagct ccccggtgtc gcggaggtcg cgatgcagtc gggcatccac gcggcggaga
     4321 cgatcaagcg ccgcgtgacg ggcggcggcg acagcaagcc gttcaagtac cgcgacctcg
     4381 gcagcatggc gagcatcgcc cgcttcagcg cgatcgtgga cttcaagggc atccggatgt
     4441 ccgggttcat cgcgtggctg atgtgggtgt tcgtgcacct ggccttcctc accggattca
     4501 agaaccgctt catcgccctt tccaagtggc tgaccgcgtt catcggagag tcacgtgacg
     4561 aacgcacggg ctcggcgctg caggcatcgg ctcgcgtgat cgtggcggcc gccggtgtca
     4621 agcccggtga gcaggacatg accaggctgg ccgagcgcga gcaccgcccc tagccggagc
     4681 ggagcgcgcc tcaggcggcg accacgggct cggcgggctc gggcgccggg gactcgatgc
     4741 ggtcctggtc gcggtactcg gcgagcagct gctgctcacg gtccttcttg gggaacacga
     4801 agaagacgag cagcgcgccg agcaggaccg cgatgatgcc ggccaggtag gcccactggt
     4861 ctccctgcag gaaggccgtc ttggccccgg cggtgatctt gtccgcatac tgcgggtact
     4921 gcttcgcgat cgcctcagct ccgtcgaatg acttcgtgag gctgttctgc acgctgctcg
     4981 tgatgttctt gccggatgcc gcgatctgac tgccggccga agttgcgtaa ccggcggtga
     5041 gcagcgctcc gaagatcgac tgcatcaccg cgccgccgag gtcgcgctgg aggtcggcgg
     5101 tgcccgacgc catgccgacc cgcttgaccg gcacggagcc ggtgagggag tgcgacgcgg
     5161 gcgtgccggc caggccaacg ccgattccga cgagcgcgta tccgaggccg accttccacc
     5221 agtcgatgtt ctccttccac agaagcagca tcgcgaggaa gccgagcagg acgaacacat
     5281 agccgcagag cagcgtgaaa cgggcgccgt gcgcctcgac tagcttggcc gagcgcgggg
     5341 cgaccagaac caagaagagc gcagccggaa ggatcgatgc tcctgccccg agcgccgagt
     5401 agccgagcac gttctgcagg aactgctggc cgatgaacat ggcgcccatg agtgagccga
     5461 aaacgatgat tcccgccgtt gcagcgaccc agaacggcgg ccgcgcggcg actttgaggt
     5521 cgtagagcgg gtttctcgcc ttccgctccc ggaggtagaa gaccaccacc gcgatcgccg
     5581 ccacgccccc gagtatcagc gactgcgttc ccttgccgtc gacgggcgcg aagttgatcg
     5641 agaggatcaa cgccgcgacc aggaggacgg acacgatccc gccgaggttg tccacggctt
     5701 cgctgccctc gttcacgtgg gccgggacgt aacgccaggc catgtagatc gagaccagcg
     5761 cgagcggcag cgtgatcacg aacacggagc cccagtcgaa gtgctcgagc agcaggccgg
     5821 cgaggacggg cccgagcgcc gcgatcgcgc cgcccagccc ggaccagagc gcgatcgctt
     5881 tggtccggcc ggcctccggc gcccacagcg ccgtgatcag cgacagagtg gtcggataag
     5941 ccatgccggc cgcaagcccg cctccaaagc gggcgatgaa gagcaccgtg tccgatggcg
     6001 cgaacgccgc cagcagcgac atcggaatgg ccagcaccgt ccctccgagg agcatcgact
     6061 tgcgtccgta gcgatcgccg agcgctccta gccacaggac cgaacacgcc aggcccagcg
     6121 agtagcccac cgcgatcagg tcgagcatcg tctggctcga gtcgaagtgc cttccgatgt
     6181 cgggcaacgc gacgttcgcc accgagaggt tcagatttgc gaccgccgcg acgagaatca
     6241 ggcttgcgag tacgagcccg gcccggttcg gagcacccgt cgtcggtttc gaaacgacag
     6301 cgctcatgcg accggcccag tctgcgggcc gagcgaagcg cctacatcat cccgaatgga
     6361 tgatgtagcg gacctggctt gcggcttcga tgaggtggcg agcccgtgct tccgttcgcc
     6421 tccacaacgg tcgatctcgc gcgcattcaa ttcgcgacga cgtcgatcta ccacttcctg
     6481 ttcgttccgc tgacgctggg actcgcaccg ctcgtggcgg tgatgcagac gatctggcat
     6541 cgcacgcacg acgaagcgtg gctgcgcctc acgcgcttct tcggaacgct cctactgatc
     6601 aacttcgcca tcggcgtggc cacgggcctc gtgcaggagt tccagttcgg gatgaactgg
     6661 tcggtgtatt cgaacttcgt cgggggcgtg ttcggcgcgc cgcttgcgat cgagggtttg
     6721 gccgccttct tcctggaggc gacctttctc ggactgtgga tctttggatg gggccgcctg
     6781 tcgccgcgca tacacctcgc aacgatctgg ctggcggcgc taggcacttg gctgtcggcg
     6841 tatttcatcc tcgtcgccaa ctcgtggatg cagcacccgg tgggctacaa ggtcgtgaac
     6901 ggcaaggccg agctcaccga cgtgggcgcg ctgctgtcga acagcttcgc gcttcgcgcc
     6961 tacctccacg ttctgctcgt cggtctcacg acggcttcca tcgtgatgct cggcatctgc
     7021 tgctggcacc tgcttcgcgg gcgcaacgtc gagctcttcc gaagagcggc gaagctcgcg
     7081 ctgatcgtgg cgttgccgat cacgctgatc aacctcatcg tcggcaatcg cttcggcgag
     7141 gcggtcacga gcgcccaggc gatgaagatc gcctccgccg aggccctttg gaacaccgag
     7201 cagcccgcgg gcttctccct gttccaggtc ggcgggttct cctcccagga ccccacgccg
     7261 agcgtctcgg tcacggttcc gaggctcctc tcctacatgg ccaccggatc gttcaacggc
     7321 aaggtcgagg gcatcaacca gctccaggcg caggagagcc agcgtttcgg cccgggcaac
     7381 tacatcccgg acgtcagggt gatctactgg tcgatccgcg tgatggccta cctcggcgtg
     7441 ctgatgttcc tcgtcgcggc ggtcggggct tggctctatc gcaagcggag gctcgagaaa
     7501 gcgcgctggt tcctctggtc cgccgtcgtc gcgatcgcgt tcccgttcat cgcggccatt
     7561 gcgggctggc tgctcactga gatggggcgc cagccgtgga tcgtccaagg cctgctcaag
     7621 acctccgagg ccaactcgcc gaatgtcagc accgcgacga tcggcacgag cctgggtctg
     7681 ttcgttctgc tctacatcac actcgggatc gtcgacttcg tcctcatgcg gcggtacgca
     7741 cgcgtcgacc cgccggagat cgaggcgccc gcgcccgccg gcgcggcgtt gggctactga
     7801 ggcggcggcg atgggtctcg aggtcttctg gttctgtctg atcggggttc tgtggggcgg
     7861 ctatttcctg ctcgagggct tcgacttcgg cgtcggcatg ctgctgccgc tgctcggcag
     7921 gaaggaaagc gaccggaaga cgatgttcga gaccatcggg ccagtctggg acgggaacga
     7981 ggtgtggctc gtcgtggcag gtgccgccac gttcgccgcg ttccccgtct ggtacgcgac
     8041 gatgttctcg ggcttctacc tcgccctcgt gatcgtcctc ctcctcttga tcgtccgcgt
     8101 ggtctcgttc gagtggcgag agaggagcga gaacccgcgc tggcgcgcga gctggcagtg
     8161 ggccaacacg gtgggcagca tcggggcgcc attcgtgtgg ggcgtcgcgc tcgcgaacct
     8221 catctacggc gttccgctga acggggacgg tggctacgcg ggagatttcc tcgacctctt
     8281 cagcgcgtac accgtcttcg cgggcgtcgt cgtggtgttt ctcttcgcgc tccacggcgc
     8341 gacgttcctg acgatccgcg cgacgggctc cctgcgcgag cacgccggca cgctggcgcg
     8401 gcggcttgcg attcccgtgg cagtgctcgc tgcgggcctg cttgcgtgga cggttgcggt
     8461 cgcccacgat cacaacaagc gcggcgtgct gcctccggcc attcccgccg caatcacgct
     8521 gggtactctc ctgctcgccg tggcgttcgt gctcgcgcgc aggcacggtt gggcgtttgc
     8581 gatgaccgcg ctcgctgcgg tcggcgtggt cgccaccatc ttcaccggcc tctatccaag
     8641 ggtcctggtc tctgacccga cgttcgcgaa cagcctgacg atctcgaacg ccgcgtcgtc
     8701 gcactacgcg ctcaaggtga tgacgatcgt ggccgccatc ttcaccccgc tcgtcctcct
     8761 gtaccaggcg tggacctact acgtcttacg gaggcggctg ggcggcgcca ccggttgagg
     8821 ttgcggcatg cgcaccctgg atcagcgcct gctgcgccgc acgagggcaa cgcggccgct
     8881 gctcgtgctc gatacggcgc tcgggatcgc gactgtcgtg cccgtgatcg cccaggccac
     8941 gcttctcgcg cacatcgtga cgcgcgcgtt cgacggcgcg tcgctgcccg ccctgcggct
     9001 cgatcttggg ctgctcctgc tggcgttcgc cgcgcgtggc gccctggcgt ggtcgatgga
     9061 ggtggccggg cggcgcgcgg cctcgagcgt actgtcggag ctccggcttg agctcgcggc
     9121 gcgccgactg acccaccacc ccgcggctga agacggcgtc gaggcgggcg agatcgccgc
     9181 cgcagccgtc cagggagtcg acgccctcga ggcgtacttt gcccgctatc tgccgcaggt
     9241 cgtgctggcc tgcgtggtgc cgatcgcggt cgtcggctgg gtcgcgtcga tcgatcccct
     9301 ctcggcgctg atcatgctgc tgacgctgcc gctcgtgccg gtgttcatgt ggctgatcgg
     9361 ccgctacacc gagcagcgga caagggagcg ctgggacgcg ctccggagcc tctccaccca
     9421 tttcctcgac gtcgtgcgcg gcctgccgac gttgagggcg ttcaatcgcg ctgtcgccca
     9481 ggcagattcg atcgccgacg tcagcgagcg ctatcgcaag gcgacgatgg gcactctccg
     9541 cgtgagcttc ctctcgggat cggttctcga gctggcggcc accctgggcg tggcactcgt
     9601 ggccgtgacg atcggggtga gactggtgga cggcgggctg gggctcgcgg ccgggctcac
     9661 ggtgctcgtg ctcgcgcccg agctgtacct gccgttcagg cgccttggcg ccgagtacca
     9721 cgcgagcgcc gacggcctgg ccgtcgcgga gcgcatgttc gggctgcttg gcgcgccgcc
     9781 cgccgtgcgg gccgtaggag ccgttgctcc ctacaacccg gctgagttcc cggtccgttt
     9841 cgagtcggtg tgcttctcgt atccgacccg gccgggccgc gtcgtcgacg gactggacat
     9901 ggagctcgcg ccgggggaga cggtggcgct tgtgggcgag agcggctgtg gcaagagcac
     9961 cgtcgccgcg ctgcttctgc gcctgatcga gccgacgggc gggcgcatca ccgtgggcgg
    10021 cgtggacctc gccgtctgct ccaccgatgc ctggcgccgg ttggtggcct gggtgcccca
    10081 gcacccctcg ctcctgcgcg ggacggtgac ggagaacatc cggctgggcg ttccgggcgc
    10141 ctccatagac cgggtgcgcg aggccgcccg gctggccggc gcggacggct tcatcgaggc
    10201 gctcccggag ggctacgaca cgatcattgg cgacggcggc cggcgtctgt caccgggaga
    10261 gcggcggcgc gtcgggcttg cccgcgcgtt cctgcgtgac gccgcgctcg tgatcctcga
    10321 cgaacccacg gccgatctcg accccgagag cgtcgagcgg gtcgccgacg cggtggagcg
    10381 gctccgggac ggcaggacga tgctcctcat cgcccaccgt cccgagctcg tcggccgcgc
    10441 cgaccgcgtc gtgcttctcg agtccggcgc agccgtgccg ttgcggatga gggaggccgc
    10501 gtgacggcga ccctgcggcg cctgctcgtt ctcgccgacg ctccgcgcgc gcgggtcgga
    10561 ctcacagtcg tgttgggcgc cctcaccgtg ctgttcggcg cggggctgat gggcacggcc
    10621 ggctatctga tctcgcgggc ggccgagcgc ccggcgatcc tctccctcac cgtcgccatc
    10681 gtcgccgtcc gcttcttcgg catcggccga cccgtcgccc ggtacctcga gcgcatcacc
    10741 tcgcacgacc tcgccctgcg cgtcctcggg cgggttcggg tgcgagtctt ccgccgcatc
    10801 gagccgctcg cgccagcgca gctggagggc tatcggagtg gagatctgct gtcgcgaatg
    10861 gtcgccgatg tggactcgct ccagaatctg cacctctgcg gcgtgagtcc gccgctcgtg
    10921 gccgcgctcg ccggcgtggt ctcggtgggt gtggcggcgg cgttcctccc tgccgccgcg
    10981 ctcgtgctcg ggtgcggcct gctggtcgga gccgtggccg tgcctgcggt ggcaggttgg
    11041 ctgggaagtc gggctgctcg caggcaggcc gctgtccgcg ggcacttgtc cgccgagctc
    11101 gtcgagctac tcgacgcggc acctgagctc gtggtgcatg gagccggcga ggcggccctc
    11161 gctcgactcg gcggggtcga ccgatcgctt gtccggctcg cgcggcggga cgcggccgcg
    11221 ggcggagtgg ccgacgggct ctgtctgttc atcaccggcg tgacggtcgc gggagtgctc
    11281 gcgaccgccg tgcatgcgtc cgcgtccggc cacttggggc gtgtgctgat cgcaatgctc
    11341 gccctgctcg cgctcgcgtc cttcgaggcg atccagccac tgtcggcagc cgcgcgcgag
    11401 ctgggcgcaa cgctggcggc cggacgccgg ctgctcgagc tcgtcgaccg ggcccccgcg
    11461 gtcgtcgatc cgcagcagcc gctgccggca ccgcgggctc cgttcgccgt tgcgctcgaa
    11521 gaggtgcggg cgcgctattc gccgcgcgag cggccggcgc tcaacggttt cagtctccac
    11581 ctcgagcccg ggcgccgcgt ggcgctcgtc ggcccaagcg gagccggcaa gaccaccgtc
    11641 gtgaacctgc tgctccgctt tctcgatccc gaggccggca gggtcacgct cgcgggcagg
    11701 gatctgcgcg actatcggca ggaggacgtt cgccgagcca tcgccgtggc aggtcaggag
    11761 tcacacctgt tctccgccag catccgcgaa aacgtgcgcc tggctcgacc cgccgcctcg
    11821 gacgccgagg tggaagacgc tctccgccac gcccgcatct gggactgggt cgacacgctt
    11881 ccggatggct gggacacccg ggtgggcgag acgggccgtg agctctcggg tgggcaagcg
    11941 cagcgggtgg tcctggcgcg cgctctcctg gcggacgcgc ccgtgctcgt gctcgacgag
    12001 ccgacggctc acctcgaccc cgctaccgcg acggagctcg tgtccgacat cttcgctgcg
    12061 ggagtcggcc actcgatcct gctgatcacc catcgtccgg agggtctcga cctggtcgac
    12121 gagatcgtca cgctctgaat cgaggtctca aatgaaatct ggctggccat tccgaagcgt
    12181 cctagccggc accgcgggca cagcggctct gacgcttgcg tacgcgacgg agcgacggct
    12241 gcgtccacgc cacacaggac cgttggacta cgacgacagc ctggtgccgg gcgagatcgt
    12301 cgccagcgtc atgcatctcc cgcacgtaac cgacagaggg gatgaggagc taggcctttt
    12361 gcttcgctgg ggctacggct cggcttttgg cctgctgcac ggactgctgc gacggcgcgt
    12421 cggcgagccg tgggcggcgg gcgcctttgg cgggacgctc atgaccgcca cgctgactct
    12481 ctttccgctg ctcggacgca ccccaccccc gtggcgatgg ggagcggacg tggtggccac
    12541 gtgcgtcggc acgcacttcg cctacgcggc atcggtggcg accgccgaca acgcaatgag
    12601 gcggcgataa gggtcgatgc tctcgactga cgacccacac tctttcgaga cccgggccag
    12661 cgccgccgtg ggtgtggcca tctcagtgac cacgattccg gcgtcggcct atctcggggt
    12721 cggcgtggga gagacttcga aggcgctcgg cgcgctgctc gtgctcggca tcaacgtgag
    12781 catgctgacg ctgggcggct ccttgacgct tctgctgcag cgatggctgg cgcggcatga
    12841 gggcttgagc ccccgctgac ccagaacgcc gaaggccact ctttcgagcg gccttcgtgt
    12901 cttggtcggc cttccaacag gctctttcga gtttctgcct tccggtcggc ctcgatagcg
    12961 ggggcgggat tcgaacccgc gaccttcgtg gagctacctg attgctccac cccgttcgca
    13021 gggcgaacgt accggaactg cggtcccaac gtcaagaccc tgagcggctc gaagcacggc
    13081 aacgttcgtt ttagcggcga gttagcgaaa cgacgaaggc cgccctttcg agcggccttc
    13141 gggtctcggt cggccttcca gcggtctctt tcgagtttcc accttctggt cggccttgat
    13201 agcgggggcg ggattcgaac ccgcgacctt cgggttatga gcccgacgag ctacctgact
    13261 gctccacccc gcggcgtgga cctacgaatg tagcgaatct gaacgcccac cgtcaaccgg
    13321 cgaaacaggc tctagaccgc ggatttttgc gccacgagag cggccgctga acccgcgctt
    13381 ggttctgggt tccccgggta tcgtgctcgc ggagcgcaac gccaacttgg aggtggacca
    13441 tgcggcgctt gctcggcatg gtttccgtcg ttttgggctt ggggctggct gccgtccctt
    13501 cggccggcgc gacagtcgat ccgttgacga gggcgagcgg gtcgtcgccg ttcgcgccgg
    13561 gatgcaacgt gtcgaccctc gggacggttt acccgaactc ggaggtggag ccctacgtcg
    13621 cctccaatcc ggtggacacg aacgacctgg tcggggtctg gcagcaggac cgatggtcca
    13681 gcgccgccgc ccagggtcag ctgacggccg tctcctctga tcgaggtagc acgtggacgc
    13741 tgcccgcgcc gccgccgttc agccactgcc agggcgggaa cgcgaccaac ggcggcgact
    13801 acgacaggac cagcgacccg tgggtgacgt tcggcccgac cggcgacagc taccagagct
    13861 cgctcgccgt ctccaacgac caggtcacca gcgcaatact cgtcagcaag tcgagcgatc
    13921 acggccagac atggggaccg atcgcgacgc tcaggcggga tgacgatccc cacttcctga
    13981 acgacaagga gtcgatcacc gctgattccc tggccccgaa ccgcgcgtac gcggtctggg
    14041 accggctctt cgccctcgat ccgacgaatc cgaactcgga cttcttcgga cccatctggt
    14101 tcgcccgaac gaccaacggt ggcgcgacct ggcggagggc tcacaagatc ttcgatccag
    14161 gcctcgacaa ccagacgatc ggcaatcaga tcgtcgagct gcccgacggt gagctgctcg
    14221 acgtattcga cctgatcatc caggggaacc tgaacgtcgc gctgattcgc tcgcccgatc
    14281 agggcaggac ctggtcgcag ccaacgatcg tggatcagga ggcctcgatc ggggtcgtcg
    14341 accccatgga tgcagcgcca gtccggaccg gagacatcat cccggaggtc gcggtcgacc
    14401 cccggcccgg cacgaacaat gtctacctgg tgtggcagga cgcccgcttc agcgccggcc
    14461 acgcggatca ggtcgtcttc acgagctcga gcgacggcgg gcgcacctgg accccggtca
    14521 cgcggatcag ccaggacggg attctgcagg cgttcacgcc gatgatcacg gtcgctggga
    14581 acggcgacat cgcggtgacg tactacgact tctcgttcga cacgccggcg tcgcccacac
    14641 tcgacaccga ctactgggtc acgcgctcca ccgacggcgg tgcgaccttc cagccccgcg
    14701 agcggatcac tcccacctcg ttcgacatgc gcacggcgcc ggtcgccagg gggttcttcg
    14761 tgggcgatta cgaggggctg gcgccgtccg cgcagttctc accgttcttc gcggcggcga
    14821 actcgggcga cctgagcaat ccgaccgacg tcttctcgac gctcgtccac gctccgttcc
    14881 caacggctgc cgcaaccgtt cgccggccac gggcgcaccg cgcgaagttc gcgcgccgct
    14941 cgccggggca catcgcagca agcaggaggc cggtccgcgt ccgctagagc gcggccgctg
    15001 ttgccgcctc gataaccggc tcgttgggcg gcacgtcgct ggcgtcatcc gacatgtacg
    15061 cccacaccac cgacccgtcg cgcgcgacca ccaacacccc tccgagctgc gccacatgac
    15121 cgacggggcg tccctggtgc acgtggtcgg tgagcgaccg cttgagcccg cgtgcgacca
    15181 ccttcggccc tagcagctcg ttgaagttcg ccaccttcgc gccggccgcc ttgtaggtct
    15241 tgcgctcggg gttcgaccag cagcgtcagc ccctccaccc cttgctcgcg caggaagtgc
    15301 tgcgcgtgcg cgggcgtgcc ctgcccgatc acgacgaggc gcgcgccggc ggcgtcgaac
    15361 ttctcacggt cgcgatgcaa ctgcaccgcg tgcgctcggc agaacgttca gccgtagtg
//
LOCUS       DASVXS010000204         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_550208, whole genome shotgun sequence.
ACCESSION   DASVXS010000204 DASVXS010000000
VERSION     DASVXS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550208"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF752_11355"
     CDS             <1..268
                     /locus_tag="VF752_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856186.1"
                     /translation="LAVSPRAPADVAGFQGRLRACRKPPVDLQMTGVTCRTGRRFHRR
                     FAPAPPGRTEVVLGFTCQVAKSRTSPGLRLTCVRGHRAVRFRFF"
     gene            complement(265..1437)
                     /locus_tag="VF752_11360"
     CDS             complement(265..1437)
                     /locus_tag="VF752_11360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8856187.1"
                     /translation="MTALWVYAATRLWATRVPGDLHLPHVAPRSLLSAHDLDEARSFD
                     RFIRFEFVASKIALIAALVWYARNGLRFARESAAGRIGTGMLLAMLGLAIVWIVQFPF
                     RLAGHWWARKHGIAEQGYLEWLLGDWFSLGGRFVFVCAGIVIVMGLAGPLKRFWWVAA
                     VPVVAGLALLFALASPYLTPNLERLSNPALAADARELARQESVPGTPVSVMKVRDFTS
                     APNAGALGFGPSRRVVLFDTLVDSFPRREVKVVVAHELGHITRGHAWKRIAFLALLVL
                     PGAAIVEAVTRQRGGLRAPRAIPLALLVVAVLQLAALPLDNAFSRRLEAEADWVALQT
                     TRDPVAARSLFARFGREAHDDPAPPGWAVALLGDHPSLTRRVAMAEAWQERQASAR"
     gene            complement(1485..>2495)
                     /locus_tag="VF752_11365"
     CDS             complement(1485..>2495)
                     /locus_tag="VF752_11365"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX8856188.1"
                     /translation="PVDLEVGEARQQDRVRVGFRLFLALPALLLASALASAGWFSGYA
                     NRGGAENDTWASWGGSVITIAAVGAWFLIMAQGRMKPGLRDLIAYCIGYAAQAWGYLF
                     LVTDRYPSADPAKVGNFQPLPEHDVNLRLTDDLQRTRLTVFFRLFLAIPHFIWLALWG
                     IAVFFALIVNWFVTLFSGTPSDSLHRFNSRYLRYQAHVFSFFLLIANPFPGFTGEPGT
                     YPVDVELPAPRRQNRWVTGFRIFLWLPAAVLTGVLDNVAWLIAFFTWWYAMFTGRAPE
                     GLRNLGAYVIRYYAQANAYLYLITDRYPYSGPWATREQSREPVVTEPPPPAAPEPEPY
                     PA"
BASE COUNT          413 a          869 c          901 g          312 t
ORIGIN      
        1 gctggcggtc agtccgcggg cgccggccga cgtcgccggc ttccagggtc ggctgcgcgc
       61 ctgccgaaag cctccggtcg atctgcagat gaccggcgtg acctgccgga cagggcgccg
      121 cttccaccgc cgcttcgcgc ccgctccgcc gggtcgaacg gaggtcgtgc tcggcttcac
      181 ctgccaagtc gcgaagtccc gcacctcgcc cggcttgcgg ctcacatgcg tccgcggcca
      241 ccgagccgtg cggtttcgct tcttctagcg ggcgctcgcc tgccgctcct gccacgcctc
      301 cgccatcgcg acgcggcggg tgagcgacgg gtggtcgccg agcagcgcga cggcccagcc
      361 aggcggcgcg ggatcgtcgt gcgcctcgcg cccgaagcgc gcgaacagcg agcgcgcggc
      421 gaccgggtcg cgcgtggtct gcagtgcgac ccagtcggcc tcggcttcga gcctccgcga
      481 gaacgcgttg tcgagcggca gcgcagcgag ctgcaacacc gccacgacga gcagggccag
      541 cgggatcgcc cggggcgctc gaagaccgcc gcgctgccgc gtgactgcct cgacgatcgc
      601 cgcgcccggg aggacgagca acgcgaggaa cgcgatgcgc ttccacgcat ggccgcgcgt
      661 gatgtggccg agctcgtgcg ccaccacgac cttcacctca cgcctcggga agctgtcgac
      721 gagcgtgtcg aagagcacca cgcggcggct cggcccgaag ccgagtgcgc cggcgttcgg
      781 cgcgctcgtg aagtcgcgca ccttcatcac cgacaccggg gttccgggca cgctctcctg
      841 gcgcgcgagc tcgcgtgcgt ccgcggcaag cgcggggttc gagagccgct cgaggttcgg
      901 cgtgaggtag ggggacgcca gggcgaacag gagcgccagt ccggcgacaa cgggaacggc
      961 ggccacccac cagaagcgct tcagcgggcc ggcgagtccc atcacgatga cgatccctgc
     1021 gcatacgaac acgaagcggc cgccgagcga gaaccagtcg ccgagcagcc attccaggta
     1081 gccctgctct gcgatgccgt gcttgcgcgc ccaccagtgg ccggcgagcc ggaaggggaa
     1141 ctgaacgatc cagacgatcg ccaagccgag catcgcgagc agcatccctg tgccgattcg
     1201 gccggccgcc gactcgcgag cgaagcgcaa gccgttccgc gcgtaccaga cgagagcggc
     1261 gatgagcgcg atcttcgatg ccacgaactc gaagcggatg aagcggtcga acgagcgcgc
     1321 ctcgtcgagg tcgtgagcac tcagcagcga ccgcggggcg acgtgtggga ggtgcagatc
     1381 gccgggcacg cgcgtcgccc acagtcgcgt ggccgcgtac acccaaagtg cggtcaccag
     1441 cgccagcccg agcgcggcga ccaggcggat atcgcgcgtt cgggtcaggc cgggtacggc
     1501 tccggctcag gcgccgccgg tggcgggggc tcggtcacca ccggctcgcg cgactgctca
     1561 cgcgtcgccc acgggccgct gtacggatag cggtcggtga tcaggtacag gtaagcgttc
     1621 gcctgcgcgt agtagcggat cacgtacgcg cccaggttcc gcagcccctc gggcgcgcgc
     1681 cccgtgaaca tcgcgtacca ccaggtgaag aaggcgatga gccaggccac gttgtcgagc
     1741 acgcccgtca ggacggcggc aggcagccag aggaagatgc ggaagcccgt gacccagcgg
     1801 ttctggcgcc gcggcgccgg cagttcgacg tcgactgggt acgtgccggg ctcgcccgtg
     1861 aagccgggga acgggttcgc gatcagcagg aagaacgaga acacgtgtgc ctggtagcgc
     1921 aggtagcgcg agttgaagcg gtgaagcgag tcggacggcg tgccgctgaa gagagtcacg
     1981 aaccagttca cgatcagcgc gaagaacaca gcgatgcccc acagcgccag ccagatgaag
     2041 tgcgggatcg cgaggaagag ccggaagaag acggtcagcc gcgtgcgctg gaggtcgtcg
     2101 gtgagccgga ggttcacgtc gtgctcgggc agcggctgga agttcccgac cttcgcgggg
     2161 tcggcgctcg ggtagcggtc ggtgacgagg aacaggtagc cccacgcctg agcggcgtaa
     2221 ccgatgcagt atgcgatcag atcgcgcagc cccggcttca ttcgcccctg ggccatgatc
     2281 aggaaccacg cgcccaccgc ggcgatggtg atcacgctcc cgccccagct cgcccacgtg
     2341 tcgttctcgg ccccgccccg gttcgcgtac ccggagaacc agccggccga cgccagcgcg
     2401 gaggccagca gcagcgccgg cagtgccagg aacagtcgga agccgacgcg cacgcggtcc
     2461 tgctggcgcg cctcgccgac ctcgaggtcg accgg
//
LOCUS       DASVXS010000205         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_240039, whole genome shotgun sequence.
ACCESSION   DASVXS010000205 DASVXS010000000
VERSION     DASVXS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240039"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF752_11370"
     CDS             <1..523
                     /locus_tag="VF752_11370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8856189.1"
                     /translation="FELKLSARDPGGRRLRSSAKPSNVGNLSFHGHRFPLIGNFDYGQ
                     SPDGRFGAPRNGHTHQGQDIGAPEGTPVVAPRGGRVSYVAYQAGGAGHYVILDGDGED
                     RDYAFMHLKAGSIVVKPGDHVLTGQKLAEVGSTGESTGPHLHFEVWEGGHWWDGGKPV
                     DPLPYLRRWDSWS"
     gene            659..970
                     /locus_tag="VF752_11375"
     CDS             659..970
                     /locus_tag="VF752_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856190.1"
                     /translation="MTIAQVLTLKLDGITAGDYLAWVHDPKPAALDHGLHSLEVRAGA
                     GDDEVEALLWWTIPAPSPLVAARAAGLPLTPEVVELWSRTLAVVSSRRDARVVPAARA
                     A"
     gene            1050..1400
                     /locus_tag="VF752_11380"
     CDS             1050..1400
                     /locus_tag="VF752_11380"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HEX8856191.1"
                     /translation="MIESQRASERITNEVTSWAGVEAGPGRRGEFAFRVGRREIGHLH
                     GDHAAHFSFPKDVWAELFAEGRITHHPVFPGKVGPGARRIESEDDVRDVIRLMRLNYD
                     RAVKRQGAPAATGT"
     gene            complement(1387..2880)
                     /locus_tag="VF752_11385"
     CDS             complement(1387..2880)
                     /locus_tag="VF752_11385"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX8856192.1"
                     /translation="MRRRFLALVAVAIAAALAGCGGGGGSALSDSLAYIPKDTPFAVG
                     IATDVNGTQYDNVNGIVKRFPFSDRIVESVKQQIKAQGLDYDKDLKPILGNEFVVGAV
                     DARSFVGDGSNRFVGALKAKDSGKLEGLVKRDSKKIGEEHGATLYQGQNSTDVVAVKD
                     GTLIVAGNRADLSAALARADGGSHVDASAFDSALDGLPEDSIVRGYFDVAKLLQSNPG
                     SADARKVKWISALRTLGFTASAEKDAIDVDFNIKTDSSGLTDADLPLATGGAPAPVIT
                     RAGDIAIGLRNPAQVLKFAQSTAQAVNPRKFGDFETAKAQVGKQLRIDVDKDVIDQLN
                     GDSSLIVSTDGKFAFRATPKNPATFAKMVTKVGKALPRIARTFGTKNIGVARPKKGES
                     FYAVATPEGESYVFGVVKGEFVLANDPKRAGQLAAGTPQEVPGGRGSLTTGSDARAIA
                     RRLIDKAASGGRGGLAAGAFVQPLGQLVGWVTTSTSGMRGTLKLTFQ"
     gene            complement(2908..3300)
                     /locus_tag="VF752_11390"
     CDS             complement(2908..3300)
                     /locus_tag="VF752_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856193.1"
                     /translation="MSQLIHTCYRIGDIDRSVAFYEALGFEEVGRLPIRDEAINVYMG
                     LPGDGARLELTYNFGVDSYELGTGYNHIAVTIEDLDGALERLREKEIEPEKPPYTVRD
                     GGSRLCFVRDPDGYRIELIESQPAPGDD"
BASE COUNT          499 a         1204 c         1124 g          585 t
ORIGIN      
        1 gtttgagctg aagctgtcgg cgcgtgaccc cggcggccgg agactgcgct cgagcgccaa
       61 gccgagcaac gtgggcaacc tcagcttcca tgggcatcgg ttcccgctga tcggcaactt
      121 cgactacggc cagagccccg acggccgctt cggggcgccg cgcaacggcc acacccatca
      181 gggccaggac atcggcgccc ccgagggcac ccctgtggtg gcgccgcgcg gcgggcgtgt
      241 ctcgtacgtg gcgtaccagg cgggcggtgc cggccactac gtgatcctcg acggcgacgg
      301 cgaggaccgc gactacgcct tcatgcacct caaggccggc tcgatagtgg tcaagcccgg
      361 ggaccacgtg ctcacgggcc agaagctcgc cgaggtgggc tccaccggcg agtcgaccgg
      421 accgcacctg cacttcgagg tctgggaggg cggccactgg tgggacggcg gcaagccggt
      481 cgacccgctg ccctacctgc gccgctggga cagctggtcc tgacccagtc gggtctgtgc
      541 gccccgaccg ggtaaatgca gacggtggtt aaccggccac gctgaagctg gctggctccc
      601 atgcgtctcg agggtgctcg cggcaccatt gcgatacccc gactatcagg actcgatcat
      661 gactatcgcc caggttctca ccctcaagct ggatggcatc acagccgggg actacctcgc
      721 ctgggtgcac gatccgaagc ccgccgccct cgaccacgga ctccattcgc tggaggtccg
      781 cgccggtgcg ggcgacgatg aggtggaagc gttgctgtgg tggaccatcc cggctccctc
      841 ccccttggtc gccgcgcgtg ccgccggcct gccgctcacc cccgaagtgg tggagctgtg
      901 gagccgcacg cttgccgtcg tgtcgtcgcg gcgcgacgct cgggtggtcc ctgctgcccg
      961 ggccgcctaa ggcgcgagcg cgtcgccggc ggtgcccagg accaattccc ccttggaaac
     1021 gggacccgcc tgcgcgcaga atggcgcgca tgatcgagtc acagagggca agcgagcgga
     1081 tcacaaacga ggtcacttcc tgggcgggcg tggaggccgg tccgggtcgg cgcggcgaat
     1141 tcgcgttcag ggtcgggcgc cgggagatcg gtcacctcca cggcgatcac gcggcgcatt
     1201 tctcattccc gaaggacgtc tgggcggagc tgttcgcgga gggcaggatc acgcatcacc
     1261 ccgtgttccc gggcaaggtc ggcccggggg cgcgccggat cgaaagcgag gacgacgtcc
     1321 gcgacgtgat ccggctgatg cgcctgaact acgaccgcgc ggtcaagcgg cagggcgcgc
     1381 ccgccgctac tggaacgtga gcttcagcgt gccccgcatg cccgacgtgc tcgtcgtgac
     1441 ccagccgacg agctgaccga gcggctgcac gaacgcgccc gccgccagtc cgcctcggcc
     1501 gccactcgcc gccttgtcga tcagccggcg agcaatcgcg cgagcatcgg atcccgtcgt
     1561 gagtgacccc cgcccgccgg gcacctcctg tggggtgccg gcggcgagct gccccgcccg
     1621 cttgggatcg ttcgccaaca cgaactcgcc cttcaccacc ccgaacacat agctctcccc
     1681 ctccggcgtg gcgacggcgt agaagctctc gcccttcttg ggccgtgcca cgccgatgtt
     1741 cttcgtgccg aacgtgcgcg cgatccgcgg gagcgccttg ccgaccttcg tgaccatctt
     1801 cgcgaatgtg gccggattct tcggcgtggc tcggaacgcg aacttgccgt cagtgctgac
     1861 gatgagagac gagtcgccgt tcagctggtc gatcacgtcc ttgtcgacat cgattcggag
     1921 ctgcttgccg acctgcgctt tcgcggtctc gaagtccccg aacttccgcg ggttcaccgc
     1981 ctgcgccgtg ctctgcgcga acttcaggac ctgggcgggg ttgcgcaggc cgatcgctat
     2041 gtcgccggcg cgcgtgatta cgggcgccgg cgcgccgccc gtcgccagcg gcaggtcggc
     2101 atcggtgagg ccgctcgagt cggtctttat gttgaagtcg acgtcgatcg cgtccttctc
     2161 cgcgctcgcc gtgaagccga gcgtgcggag ggcgctgatc cacttgacct tgcgcgcgtc
     2221 ggccgagccc gggttgctct ggagcagctt ggcgacgtcg aagtagcccc ggacgatcga
     2281 gtcctcgggc agtccgtcga gcgcggaatc aaacgcgctc gcgtcgacgt ggcttccccc
     2341 gtccgcgcgt gcgagcgcgg ccgagaggtc cgctcggttg cccgcgacga tcagcgtccc
     2401 atccttgacc gccactacgt cagtcgagtt ctgcccctgg tacagcgtgg cgccgtgctc
     2461 ctcgccgatc ttcttcgagt cgcgcttcac gagcccctcg agcttcccgc tgtcctttgc
     2521 tttcagcgcg cccacgaagc ggttcgaccc gtcccccacg aagctcctcg catcgacagc
     2581 gcccactacg aactcgttcc cgaggatcgg cttcaggtcc ttgtcgtagt cgagcccctg
     2641 cgccttgatc tgctgcttca ccgactccac gatgcggtcg ctgaacggga agcgcttcac
     2701 gatcccgttg acgttgtcgt actgcgtccc gttcacgtcc gtcgcgatcc caacagcgaa
     2761 cggcgtgtcc ttcgggatgt aggcgagcga gtcgctgagt gcgctcccgc cacctccccc
     2821 gcaacctgcc agcgctgccg cgatcgcgac agcgaccagc gctaagaatc gcctccgcat
     2881 cggcgctgca agatatcgcg ggcccgctca gtcgtcgcca ggcgctggct ggctctcgat
     2941 caactcgatg cggtagccgt ccggatcgcg cacgaagcag aggcgcgacc cgccgtcgcg
     3001 gacggtgtat ggcggcttct cgggctcgat ctccttttcc ctcagcctct cgagcgcacc
     3061 gtcgaggtcc tcgatcgtca cggcgatgtg gttgtacccc gtaccgagct cgtacgagtc
     3121 gacgccgaag ttgtacgtga gctcgagcct cgcgccgtcg ccgggcagac ccatgtagac
     3181 gttgatcgcc tcgtcgcgga tcggcaggcg gccgacttcc tcgaaaccga gcgcctcgta
     3241 gaacgcgacg cttcggtcga tgtcaccgat gcggtagcag gtgtggatca gctggctcat
     3301 gcgcatgagc gtaacgagcc gctcccgcta ccatggccgg gctgcctggc gaggtagctc
     3361 agttggtaga gcacacgact gaaaatcgtg gtgtcgccgg ttcgattccg gc
//
LOCUS       DASVXS010000206        17656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_593293, whole genome shotgun sequence.
ACCESSION   DASVXS010000206 DASVXS010000000
VERSION     DASVXS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593293"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VF752_11395"
     CDS             <1..1130
                     /locus_tag="VF752_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8856194.1"
                     /translation="ALGDRARILSPPELVEEAHERARLVAERHSGEFELAREVAPKPL
                     PLEEPEPNGKREAPIRPERFARLVTLAGILIEATRTGEPLDVGQVRESLQVSDAELRE
                     DIDLLNVVNFGGGSYVLYAEIQGDHIEVDPEPYGDNFARPATLLPLEAKALVAAIDYL
                     GEHLPQGSLASARRKIVDALGRDPADDGLQMTTPGADDPQIVRVVNQAIAKHRLLEVE
                     HYKEDEDEFSKRVLEPYKLFNTHEGWYLHAYDPKKKDTRTFKLERIRSARVLDGKFEP
                     RPDVSVDDLSWPVTGQIENSQVAKVWVAPEQAARTKEARRVFAELADGSVIAELRYAG
                     DDYLIAQILREAGDFVVLEPEDARAAVLAAAESLAGDTVRS"
     gene            complement(1138..1614)
                     /locus_tag="VF752_11400"
     CDS             complement(1138..1614)
                     /locus_tag="VF752_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX8856195.1"
                     /translation="MSALKRVGTRVRADECGFTLPEVLIVCVVLGILAAIALAQFLGN
                     SRKADDADAKSNARSLQAQVESCFTDAQDFGSCTGSGPADQLGSNLGLPMGSSAGEVH
                     VSATTQSTYEITAVSKASSGGANHRFMLSRDPVGRVQHTCVAGPTNSDGGCHGGFW"
     gene            complement(1829..3115)
                     /locus_tag="VF752_11405"
     CDS             complement(1829..3115)
                     /locus_tag="VF752_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856196.1"
                     /translation="MEAARTLQLHTSVVDVREGALWIDGLVVDDRTLIELVRRRVESG
                     VDPENTVRDALEIGARVLDREGTGAEVDFVRRELERMSSDVERGLREQFERFLGGDSG
                     EMSKALESHSEELGELIARHFGADRATAVQHQMREVVAKALQESRQDLLRQFSAQDGH
                     NPLADFKGAVIREMKASRQNGEALIAKIHSLEGEIKRLQDARRAEGELAAERERGTSK
                     GRSFEQQAYELIDEMASARGDVAHHVGDERSASGGKLGDVVVEIDAASGPAKGRIAFE
                     VKDEKLSKNRAWETLNGSLAERDAGFAVLVVASEDKVPSGREPLHEYEGNKMIVALDK
                     ESLDPRPLELAYRYARCRCLMSAEKSLEVDAAGVRDAAEEALSALRDAQKIRASLTGA
                     TNSVDNAREALNVMVERVQSAIARVESLIAAQTPTD"
     gene            3189..5564
                     /locus_tag="VF752_11410"
     CDS             3189..5564
                     /locus_tag="VF752_11410"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8856197.1"
                     /translation="MRGTGLAGVLAAGVGAGSAALWFQLFKRPLPQTSGTIEVAGLEA
                     PVRIERDQLGVPRIEAHTTADLCFGIGFLHAQERLWQLEFYRRVGSGTLAEMVGPEAL
                     QVDRMMRTLGMRRASERDAAHLSERDREVLEAYASGVNAAIAAMHAPPLELQLLRATP
                     APWTPADTLVVRRILALGFSTNMETELFRAELVRKVGIEKAAVLEPRYPRGNPLVTDP
                     GAGWTGDALEIVEQFGRVREAIGLSLEPAGSNNWVVSGDRSVTGAPLMAGDPHISATI
                     PDTWFVLEANTPELELRGGTMPGIPGLVMGQTRHVAWSFTNVLADVQDLFVERIRDHE
                     YEFEGEWRPLTVHREEIAVAGRSAEQLEVLETHHGPIVNGPLGADHGGEPLALSWTAL
                     REPMRSSVAIDLGLLKSGEELVEAFREYSSPCMNLLWADDRGNVGYKLVGFIPLRRGG
                     CPDLPKPGWTGEYEWDGYVPYDELPSIVNPPGGTIVTANNRIAPDDYPHHITSEYLDG
                     YRAARIEQLLAERDHHSLDDFERIQSDLYSIPGEQTSHRLARLRPAGQRERSAIERLK
                     SWDFVLDPDTIAGTIYQAFTVHFARLVSEAAIGDPDYAARWRAKSLLGFTAMTTSLWR
                     FQARLLELWDEADSDLIGGRSWDELALEALSEALDELEQAHGSDPARWRWGRVHGLAF
                     PHALGEGPGRISKLFDKLLSRRLAAGGGQETVSQIGFVPHHGDYTGRWAPSLRLLLDL
                     EDPDRSRWQHMTGQSGHPGSPHYDDLLDSWLAGRTNRFAQPAEETLRLSPA"
     gene            5576..6043
                     /locus_tag="VF752_11415"
     CDS             5576..6043
                     /locus_tag="VF752_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX8856198.1"
                     /translation="MPSRRDQIKMSDDEVREFLAAHKVMTVATIGPSGRPHLMPLWYV
                     PDGMVLRGWTYAKSQKAKNLERDPRATLQVEDGVQYHELHGVMLECDVDVERDPAEVE
                     KLGVALFTRYGDGGAPGPEVREMVAAQAPKRVALTFHPTRVVTWDHRKLGGAY"
     gene            6044..7120
                     /locus_tag="VF752_11420"
     CDS             6044..7120
                     /locus_tag="VF752_11420"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX8856199.1"
                     /translation="MKKLKGLILSGGKGTRLRPITYTSAKQLVPVANKPVLFFGIEAL
                     AAAGIDEIGIIIAPETGAEIREAAGDGSDFGVSITYIEQDEPLGLAHAVLTAEDFLAD
                     SPFVMYLGDNLLRDGVADLVETFRSQEPDALILLTHVNDPENYGVAELDGDGRVARLI
                     EKPHEPGSNLALVGVYMFTPAIFDAARAIEPSGRNELEITDAIQWLVNSKMRVDPHVV
                     SGWWKDTGQVADMLQANHLILDDLQEQIDGELVDSKVEGRVVIEPGARLERTTVRGPA
                     IIGAGAVLADAYIGPYTAIGPNVRIERAEIEYSIVLEGSTVEKLDGRVEASLIGKNVR
                     ITRGAMMPRAYRFVAGDNAEIMIL"
     gene            complement(7117..8016)
                     /locus_tag="VF752_11425"
     CDS             complement(7117..8016)
                     /locus_tag="VF752_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856200.1"
                     /translation="MTRVRLAVVAAAVLVLAMPSAAQAAAASPAITAKTKTVEAGKIR
                     LGYRSFGKGPPIVFVMGLFGTMDNWDPAFLDPLARHHRVIVFDNRGVGRSTNTKASFT
                     MHDMGEDAAILIKALKLDKPALVGWSMGGFIAQDVAVNHPRLISRLVLNSTGPQAPRF
                     VAPSPEVTAKLFGPLPTLAEILSTLFPPDQQAALQDWIGRFTMRRPLELVPPSTANKQ
                     KLAVAGWVASPGTDDPKRIKVPTLIGAGLEDTLVPPANSRVLAKRIPHAKLKLYPDAS
                     HAFLFQERKTWVPRIERFLKTGR"
     gene            complement(8032..8916)
                     /locus_tag="VF752_11430"
     CDS             complement(8032..8916)
                     /locus_tag="VF752_11430"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HEX8856201.1"
                     /translation="MLSAGAPASARADGIFVADCSLSHRAANDPIVFHGMPGASHMHD
                     FFGNASTNASTTLRSLRRNTGNCLPAGDRSGYWTPTLFRQGRALKPVQVQAYYQDFFR
                     YGRVLPFPPGLRIVAGSHKAKRWQRGIVRWTCQDDQGEGAARPPSCGSKFVTLRVTFP
                     DCWDGQRLDSPDHRSHMAYNREGGMEFGPQRCPKSHPIVVPQLQLNVVYPMHDGRGVL
                     LASGSVLTAHGDFFNGWKPSLLKQRVDDVLNGGKACDDFLGCVRINAPNTEPVVARPN
                     RKLVDRFYRPRTESMHHH"
     gene            9157..9993
                     /gene="rfbD"
                     /locus_tag="VF752_11435"
     CDS             9157..9993
                     /gene="rfbD"
                     /locus_tag="VF752_11435"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX8856202.1"
                     /translation="MLVTGASGMLGSDVVRAARFVNHEVVPVGHQELEITDAEAVRRL
                     FESEQPQAVVNCAAYTDVDGAEDDLEGAMAVNGDGARNVAAAAQAVGAQVVYPSTDYV
                     FDGSKSEPYLETDEPRPLSVYGQTKLAGEHETITSNPAHYVVRSSWTFGVNGRNFVDT
                     MLMLGADHGEVLVVRDQVGSPTWTGHLSDAIVRLLDGEAYGIHHIASDGECSWYEFAI
                     AIFEQAGVDSRVLSCTTDEFGRRAPRPSYSVLRSARDDTVFLPHWREGLASYLAERAL
                     AA"
     gene            9990..10988
                     /gene="rfbB"
                     /locus_tag="VF752_11440"
     CDS             9990..10988
                     /gene="rfbB"
                     /locus_tag="VF752_11440"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX8856203.1"
                     /translation="MRLLVTGAAGFIGSTFVRVVLEERPDAEVVVLDKLTYAGRRENL
                     QEVEDRIRFVQGAIEDEEVVLETMEGADAVVNFAAESHVDRSISDQYAFVRTHVIGTS
                     VLLDAARQHGVSRFLQVSTDEVYGSIEEGSFTETSPLNPSSPYSATKAAGDLLVSAHV
                     HTHGIEAVICRGSNNYGPRQYPEKLIPLSVLNAVAGDHLPVYGDGRQVRNWLYVEDFA
                     RAIDTVLRNGTPGEAYNVGGPDEEENIDVVRRILDLTGRDESLIEHVTDRPGHDRRYS
                     LSSDKVRALGWESRVRFEEGLERTVEWYRDNEWWWAPIRSGEYRDYYERQYGRSLG"
     gene            10981..11712
                     /locus_tag="VF752_11445"
     CDS             10981..11712
                     /locus_tag="VF752_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8856204.1"
                     /translation="MAEQSPALELKGLTKRYDDGTEALVDFDLVVPDGAFFGLLGPNG
                     AGKTTLISAVCNLIRITSGDVLVFGRPAADLTARSWVGLAEQDINLDRFLTVEETLIY
                     HGGWFGMNRDEAEARAAEMIDVFDLRAKADTRTPKLSGGMRRRLLLARALMHRPRLVI
                     LDEPTAGVDFELRHELWRYIRTLHGEGTTILLTTHYLEEAEELCEEIALIRGGRLIAR
                     DTSDGLRAHYGAASLADVYVKAMGV"
     gene            11709..12473
                     /locus_tag="VF752_11450"
     CDS             11709..12473
                     /locus_tag="VF752_11450"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8856205.1"
                     /translation="MTGARGLMWLSRREVLRVSKLWTQTVLAPVISSMLFILVFGLAL
                     GGRFKQVEGFDYREFIVPGLITMAMVQAAYNNNASSIFQARSDRYINDVLSAPMHAWQ
                     MNVGFNVGGFVRALWIGVSLLVLSLPLTGVPVREPLVLIVAVLLALVLFSALGTVVGI
                     FAETFDHHMFVSNVVILPLTFVGGVFYSVDRLGSPWHELSHLNPVFYLVNAVRYGFLG
                     TADVSVWLALGVTAALAIPAYAWSHWLFVTGRKLKD"
     gene            complement(12470..13447)
                     /locus_tag="VF752_11455"
     CDS             complement(12470..13447)
                     /locus_tag="VF752_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX8856206.1"
                     /translation="MPPLAPDRMMLEILEDVEVDDQLVEAVQRLKRQGYTIALDDFTF
                     SDSIKPLLEMVDIVKLDVREHDPAALEGQLRLLLPYGKKLVAEKVESYEEFEACKLLG
                     IDYFQGDFFCKPKVLRQRAIPSNRLAQLKLIAQLNSDDADFDEVEKLISRDVGLSFKL
                     LRYINSAFFSLPQKVGSVRHAMVLLGLQAIKRWSTLLVMAGVDDKPHELIVTALIRAR
                     MCELLSPSQVQREHEEHFTVGMFSVVEALMDAPMEKILASLPLSDELKIALSEQEGPK
                     GRVLGAVLAFESGDFQKLVELAPRVGVSVQDAYNQSVAWASEASRELER"
     gene            complement(13474..13716)
                     /locus_tag="VF752_11460"
     CDS             complement(13474..13716)
                     /locus_tag="VF752_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856207.1"
                     /translation="MNALPQPVPEPSAANEIFIARQPIYDRQVEVFAYELLFRDGSGN
                     SALVADGEAATARVIGNAFTEMASRRSSTTASRRSM"
     gene            13758..14423
                     /locus_tag="VF752_11465"
     CDS             13758..14423
                     /locus_tag="VF752_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8856208.1"
                     /translation="MPELADIERQVTECRRCPRLVEWREKVARDKRRAFAGEDYWGRP
                     VPGFGDPAARVYVLGLAPAAHGGNRTGRVFTGDRSGDFLFESLHRTGFANQPRSVSRD
                     DGLELRGAFVAASVRCAPPANKPLPSERDNCLPYAAAELEAMASVRVIVCLGSYAWDC
                     ACRLLGIRPKPKFGHLAMFEHERAVLLGCFHPSQQNTFTGKLTPAMMDEVFARARELS
                     DAF"
     gene            complement(14499..17060)
                     /locus_tag="VF752_11470"
     CDS             complement(14499..17060)
                     /locus_tag="VF752_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8856209.1"
                     /translation="MLATSLKGLTERKLRTFLTAFAVVLGVALISGTYILTDTISKTF
                     DNVFTEVSKGIDVAVTPHKAFNDTNQGQNPPAFNAAILKQTQQVPGVQKAVGGVFDQA
                     SIIGKDGEKVGSNTTPNFVTNVEPEPFNAFNYVSGHAPTGPGEVALDKHTAADEGWKV
                     GDKVKVLGHSAARTYTLVGIAKFGNLNSVAGAGIAVMTTPEAQKALDKVGRFDSVDIQ
                     AAPGVSADALAARVRADLPNTVTVRTGTEQARQDSQDVKDGFNFIKVLLLVFAGVALF
                     VGAFMIFNAFSITIAQRTRELSMLRTLGASRRQVLNAVTGEALIVGFTASVIGLLAGL
                     GLAPALKALFKAFGAELPSGGTVVETRTIVVSLLVGTLVTLVASLGPAVRATRISPIE
                     GVREGAEIRGGRLARMKTPLAIGISVLGIALLAFGLFGGQSGGAALGPVGGGAALLFL
                     GVALVSSRLVKPLSTVIGSPLERSFGVTGQIARENSVRNPGRTAGTAAALMIGLALVV
                     FVTIFAAGIRHSIDDAIGKSVTAGLVVENKDGWSLIPESTGQAVAKVDGVSSVSPVRY
                     SAAHAKGIGNLDGLSGIDPATFTPLFHVEWKKGSDAIASSLTDSGALASESWADSHNK
                     KIGDQVTLQTQSGKEVPVTIKATYKNNVHALGDMVITNSLMGREFGQKQAQYIFVGLQ
                     QGANADQARAQATKVVSSQFPTAEVVTKDGFKDTQTKWVNGILSFFYVLLSLAVIVSL
                     FGIVNTLVLAIYERTREIGMIRAVGGSRKQVKRIVRYESIITALIGATLGTVLGVFFA
                     IIVSRPLASEGFSLSFPIATLIILLVLAALAGMVAAITPARRAAKLDVLKALAYE"
     gene            complement(17062..>17656)
                     /locus_tag="VF752_11475"
     CDS             complement(17062..>17656)
                     /locus_tag="VF752_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8856210.1"
                     /translation="GSGKSTLMHILAGLDRPTGGDVLVEGVSLTSLDDAALTGLRRDK
                     FGFIFQFFNLLPILTAEENITLPMKLAGEKPDPAWLDELIEAVGLSDRRKHKPSELSG
                     GQQQRVAVARALLTRPAVVFADEPTGNLDSVAGEEVLAMLRRAVDDYGQTVVMVTHDP
                     TAASVADRIVVLKDGKVVNEGPAGDRSEVVDLMREVA"
BASE COUNT         2751 a         6042 c         6084 g         2779 t
ORIGIN      
        1 tggcgctcgg cgaccgcgcc cgcatcctgt ccccgcccga gctggtggag gaggcgcacg
       61 agcgcgcgcg gctagtcgcg gagcggcaca gcggtgagtt cgagctcgcc cgcgaggtgg
      121 cgccgaagcc gctgccgctc gaggagccag agccgaacgg caagcgcgag gctccgatcc
      181 ggcccgagcg gttcgcgcgc ctcgtcacgc tcgccggcat cctgatcgag gcgacgcgca
      241 cgggcgagcc gctcgacgtc ggccaggtgc gcgagagcct ccaggtctcc gacgccgagc
      301 tgcgggagga catcgacctc ctgaacgtcg tgaacttcgg cggcggcagc tacgtgctct
      361 acgccgagat ccagggtgac cacatcgagg tcgatccgga gccctacggc gacaacttcg
      421 cgcgccccgc cacgctgctg cctctcgagg cgaaggcgct cgtcgcggcg atcgactacc
      481 tcggcgagca cctcccgcag ggctcgcttg cgagcgcgcg caggaagatc gtggacgcgc
      541 tcggccgcga cccggccgac gacggcctgc agatgacgac gcccggcgcc gacgacccgc
      601 agatcgttcg ggtggtgaac caggcgatcg ccaagcaccg gctgctcgag gtcgagcact
      661 acaaggagga cgaggacgag ttctcgaagc gggtgctcga gccgtacaag ctcttcaaca
      721 cacacgaggg ctggtacctc cacgcgtacg acccgaagaa gaaggacacc cgcaccttca
      781 agctcgagcg gatccgctcg gcgcgcgtgc tcgacgggaa attcgagcca cgcccggacg
      841 tgtccgtaga cgatctcagc tggcccgtga ccgggcagat cgagaactcg caggtcgcga
      901 aagtctgggt cgcgcccgaa caagcggctc gcacaaagga agcgcggcgc gtgttcgccg
      961 agctggccga cggctcggtc atcgccgagc tgcgctacgc gggcgacgac tacctgatcg
     1021 cccagatcct ccgcgaggcc ggcgacttcg tggtgctcga gcccgaggac gcgcgcgccg
     1081 ccgtgcttgc ggcggccgag tcgctcgcgg gcgacaccgt ccgcagctag ctacccgcta
     1141 ccagaagccg ccgtggcagc cgccgtcgct gttggtcggc ccggctacgc acgtgtgctg
     1201 gacgcggccg acaggatcgc ggctcagcat gaagcggtgg ttcgctccgc cggagcttgc
     1261 cttggagacc gcagttatct cgtaggtcga ctgagtcgtc gcactaacgt ggacctcacc
     1321 ggcggaactc cccatcggca ggccgaggtt cgaaccaagc tggtcggcgg gccccgaccc
     1381 ggtgcacgac ccgaagtcct gagcgtcggt gaagcagctc tccacctggg cctggaggct
     1441 gcgggcgttc gacttggcgt cggcgtcgtc cgccttgcgc gagttgccga ggaactgggc
     1501 gagcgcgatc gccgcgagga tgcccagcac cacgcagacg atcagcacct ccggcagcgt
     1561 gaagccgcac tcgtccgccc gaacgcgcgt cccgacccgc ttcagggcgc tcatcccctg
     1621 tcttcccctg tcatttggtt ggaaatcgga cagccgggtg gcttcctcgg gtgatccgct
     1681 gtccctttgt gaggaagcct tccccggcca tccgtacccg cgaaaggagc tgtcgggccg
     1741 tgctgttggc cttcggcttg tgcgtatatc gacaaaccgc aagcgagctt tagccccacg
     1801 cgcggcgttt cgtcaaaaga agcgtgggtc agtcggtggg cgtttgcgcc gcgatcaggc
     1861 tctccacgcg ggcgatcgcc gactgaaccc gctcaaccat gacgttcaag gcctcgcgcg
     1921 cgttgtccac gctgttcgtg gcgccggtca gcgaagcgcg gatcttctgc gcgtcccgca
     1981 gcgccgacaa cgcttcctcg gccgcgtcgc ggactcctgc tgcatcgacc tcgagcgact
     2041 tctccgcgct catcaggcag cggcagcgcg cgtagcggta ggcgagctcg agcggccgcg
     2101 ggtcgagcga ctccttgtcg agcgcgacga tcatcttgtt gccctcgtac tcgtgcagcg
     2161 gctcgcgacc ggacgggacc ttgtcctcgg acgcgaccac gagcacggcg aagcccgcgt
     2221 cgcgctccgc gagcgagccg ttgagcgtct cccaggcgcg gttcttcgag agcttctcat
     2281 ccttgacctc gaaggcgatc cggcccttgg cgggaccgga ggcggcgtcg atctcaacga
     2341 ccacgtcgcc gagcttgccg ccgctcgcgg agcgttcgtc gccgacgtgg tgagcgacgt
     2401 cgccgcgcgc ggatgccatc tcgtcgatca gctcgtaggc ctgctgctcg aacgagcggc
     2461 ccttcgaggt gccgcgctcg cgctccgccg ccagctcccc ctccgcccgg cgcgcgtcct
     2521 gcagccgctt gatctcgccc tcgagcgaat ggatcttcgc gatcagcgcc tcgccgttct
     2581 ggcgcgaggc cttcatctca cggatgaccg cgcccttgaa gtccgcgagc gggttgtgcc
     2641 cgtcctgcgc cgagaactgg cgcagcaggt cctggcgcga ctcctgcagc gccttggcga
     2701 ccacctcgcg catctggtgc tggacggccg tggcgcggtc cgcgccgaag tggcgcgcga
     2761 tcaactcccc gagctcctcg ctgtgtgact cgagcgcctt cgacatctcg ccgctgtcgc
     2821 ccccgaggaa gcgctcgaac tgctcgcgca gcccgcgctc gacgtcggac gacatgcgct
     2881 cgagctcgcg ccgcacgaag tcgacctccg cgcccgtccc ctcgcggtcg agcacgcgcg
     2941 cgccgatctc gagcgcgtcg cggacggtgt tctcgggatc gacgccgctt tccacccggc
     3001 ggcgcaccaa ctcgatcagc gtgcggtcgt cgaccaccaa gccgtcgatc cacagcgcgc
     3061 cctcgcggac atcgaccacg gaggtgtgaa gctggagtgt gcgagcggct tccatctgga
     3121 ctgatcgacg gtaggaccgg tcccggacgg aacctcgcgc gtgtaagaat cggcggctcg
     3181 tgagcagaat gcgtgggaca gggctcgcag gtgtgctcgc cgccggtgta ggcgccggca
     3241 gcgcggccct ctggttccag ctcttcaagc ggccgctgcc gcagacgagc gggacgatcg
     3301 aggtcgcggg gctcgaggca cccgtccgca tcgagcgcga ccagctcggt gtgccgcgca
     3361 tcgaggcgca caccacggcc gacctgtgct tcggaattgg cttcctgcac gcgcaggagc
     3421 ggctgtggca actcgagttc taccggcgag tgggctccgg aacgctcgcg gagatggtcg
     3481 gccccgaggc gcttcaggtc gaccgcatga tgcgcacgct cgggatgcgg cgcgcctcgg
     3541 agcgcgacgc cgcgcacctg tcagagcgcg accgcgaggt cctcgaggcg tacgcgagcg
     3601 gcgtgaacgc ggcgatcgcc gcgatgcacg ccccgccgct cgagctacag ctcctgcggg
     3661 cgacgcccgc accgtggacg ccggccgaca cgctcgtggt ccgccgcatc ctcgcgctcg
     3721 ggttctcgac gaacatggag acggagctgt tccgggcgga gctggttcgc aaggtcggca
     3781 tcgagaaggc cgcggtgctc gagcctcgct atccgcgcgg caacccgctc gtgaccgacc
     3841 ccggcgccgg ctggaccggc gacgcgctcg agatcgtcga gcagttcggg cgcgtgcgcg
     3901 aggcgatcgg gctgtcgctc gagccggccg ggtcgaacaa ctgggtcgtc tccggcgacc
     3961 gctccgttac cggcgcgccg ctcatggccg gcgacccgca catttccgcc accattcccg
     4021 acacctggtt cgtgctcgag gcgaacacgc ctgagctcga gctccgaggc ggcacgatgc
     4081 ccggcattcc cggcctcgtc atgggccaga cgcgtcacgt ggcctggagc ttcacgaacg
     4141 tgctcgccga cgttcaggac ctgttcgtcg agcgcatccg cgaccacgag tacgagttcg
     4201 agggcgagtg gcggccgctc acggtgcacc gcgaggagat cgcggtggcg ggccggtccg
     4261 cggagcagct cgaggtgctc gaaacccatc acggaccgat cgtcaacggc ccgctcggcg
     4321 cggatcacgg gggcgagccg ctggcgctgt catggaccgc gctgcgcgag ccgatgcgca
     4381 gctcagtcgc catcgacctc ggcctgttga agagcggcga ggagctggtc gaggcgttcc
     4441 gcgagtactc ctcgccatgc atgaacctgc tgtgggccga cgaccgcggg aacgtcggct
     4501 acaagctggt cggtttcatc cccctgcggc gcggcggttg ccccgacctg ccgaagcccg
     4561 gctggaccgg cgagtacgag tgggatggct acgtgccgta cgacgagctg ccctcgatcg
     4621 tcaacccacc cggcgggacg atcgtgaccg cgaacaaccg gatcgcgccg gacgactacc
     4681 cgcatcacat cacgagcgag tacctcgacg ggtaccgcgc tgcgcgcatc gagcagctgc
     4741 tcgccgagcg cgaccatcac tcgctcgacg atttcgagcg catccagtcc gacctctact
     4801 cgattcccgg cgagcagact tcacaccggc tggcccggct gcgccccgcc gggcagcgcg
     4861 agcgcagcgc gatcgagcgg ctgaagagct gggacttcgt gctcgacccg gacacgatcg
     4921 ccggcacgat ctaccaggcg ttcaccgtgc acttcgcgcg gctcgtgtcg gaggccgcga
     4981 tcggcgatcc cgactacgcc gcgcgctggc gggccaagtc gctgctcggc ttcactgcga
     5041 tgacgacctc gctgtggcgc ttccaggcgc ggctgctcga gctgtgggac gaggccgaca
     5101 gcgacctgat cggcgggcgc tcgtgggacg agctggcgct cgaggcgctg tcggaggcgc
     5161 tcgacgagct cgagcaggcg cacggctcgg acccggcgcg ctggcgctgg gggcgggtgc
     5221 atgggcttgc gttcccgcac gcgctcggcg agggcccggg ccgcatctcg aagttgttcg
     5281 acaagctcct gtcgcggcgg ctcgccgccg gcggcggcca ggagaccgtc tcgcagatcg
     5341 gcttcgtgcc gcatcacggc gactacaccg ggcgctgggc gccgtcgctc cgcctgctgc
     5401 tcgacctcga agacccggac cgctcgcgct ggcagcacat gaccggccag tcgggccatc
     5461 cgggcagccc gcactacgac gatctgctcg actcctggct cgcgggccgc acgaaccgtt
     5521 tcgcgcagcc cgccgaggag acactgcgac tctcccccgc ataggctccg gcctgatgcc
     5581 atcgcggcga gaccagatca agatgtcgga cgacgaggtg cgcgagttcc tcgcggccca
     5641 caaggtcatg accgtggcaa cgatcgggcc gagcgggcgg ccgcacctga tgccgctctg
     5701 gtacgtgccc gatgggatgg tgctgcgcgg ctggacctac gcgaagtcgc agaaggcgaa
     5761 gaacctcgag cgcgacccgc gcgcgacgct gcaggtggag gatggcgtgc agtaccacga
     5821 gctccacggg gtgatgctcg agtgcgacgt ggatgtcgag cgcgatcccg ccgaggtcga
     5881 gaagctcggc gtcgcgctct tcacgcgcta cggcgacggc ggggcacccg gtcccgaggt
     5941 gcgcgagatg gttgcggctc aggcgccgaa gcgcgtggcg ctcacctttc atcccacgcg
     6001 cgtggtcacg tgggaccacc gcaagctggg cggggcgtac taggtgaaga agctgaaggg
     6061 cctgatcctg tccggcggca agggcacgcg cctgcgcccg atcacctaca cgagcgccaa
     6121 gcagctcgta ccggtcgcga acaagccggt gctgttcttc ggcatcgagg cgctcgcggc
     6181 cgcgggtatc gacgagatcg ggatcatcat cgcccccgag accggtgccg agatccgcga
     6241 agcggccggc gacggctcgg acttcggcgt ctcgatcacc tacatcgagc aggacgagcc
     6301 tctcgggctg gcgcacgcgg tgctcaccgc tgaagacttc ctcgccgact caccgttcgt
     6361 catgtacctc ggcgacaacc tgctgcgcga cggcgtcgcc gacctcgtcg agaccttccg
     6421 ctcccaggag ccggacgcgc tcatcctgct cacgcacgtc aacgaccccg agaactacgg
     6481 ggtcgccgag ctcgacgggg acggccgcgt ggcgcgcctg atcgagaagc cgcacgagcc
     6541 cgggtcgaat ctcgcgctcg tcggtgtcta catgttcacg cccgcgatct tcgacgccgc
     6601 gcgcgcaatc gagccgtctg gccgaaacga gctcgagatc accgacgcga tccagtggct
     6661 cgtcaactcc aagatgcgcg tcgacccgca cgtggtgagc ggctggtgga aggacaccgg
     6721 ccaggtggcg gacatgctcc aggccaacca cttgatcctt gacgacctgc aggagcagat
     6781 cgacggggag ctcgtcgatt cgaaggtgga gggcagagtg gtgatcgagc caggcgcgcg
     6841 gctcgaacgg acgacggtgc gcggcccggc gatcatcggc gcgggcgccg tcctcgccga
     6901 tgcctacatc ggcccttaca ccgccatcgg gccgaacgtg cgcatcgagc gcgccgagat
     6961 cgagtactcg atcgtgctcg agggctccac cgtcgaaaag ctcgatggcc gagtggaggc
     7021 gagcctgatc ggcaagaacg tgcgcatcac ccgtggagcg atgatgcccc gcgcctaccg
     7081 cttcgtcgcc ggcgacaacg ccgagatcat gatcctctag cggcccgtct tcaggaagcg
     7141 ctcgattcgc ggcacccacg tcttgcgctc ctggaaaagg aaagcgtgcg acgcgtccgg
     7201 gtagagcttg agcttcgcgt gcgggatgcg cttggcgagg acgcgcgagt tcgcgggcgg
     7261 cacgagcgtg tcttccaggc cggcgccgat gagcgtcggg accttgatgc gcttgggatc
     7321 gtccgtgccg gggcttgcta cccagcccgc cacggcgagc ttctgcttgt tcgcggtgct
     7381 cggcggcacc agctcgagcg gccggcgcat ggtgaagcgg ccgatccagt cctgcagcgc
     7441 cgcctgctgg tccggtggga agagagtcga gaggatctcc gcgagcgtcg ggagcggacc
     7501 gaacagcttc gccgtcacct ccggtgaagg cgccacgaaa cggggtgcct gcggaccggt
     7561 ggagttcagc accagccgcg agatcagcct cgggtggttg accgccacgt cctgcgcgat
     7621 gaagccgccc atcgaccagc ccaccagggc cggcttgtcg agcttgagcg ccttgatcag
     7681 gatggccgcg tcctcgccca tgtcgtgcat ggtgaagctg gccttcgtgt tcgtggagcg
     7741 cccgaccccg cggttatcga acacgatcac acggtggtgc cgcgccagcg gatcgaggaa
     7801 cgccggatcc cagttgtcca tcgttccgaa caaccccatc acgaagacga tcggcgggcc
     7861 cttcccgaag gagcggtagc ccagccggat cttcccggcc tcgacggtct tcgtcttggc
     7921 ggtgatggcc ggcgaggccg ccgctgcttg cgcagcgctc ggcatggcga gcacgagaac
     7981 ggcagccgcg accacggcca gtcgaacgcg agtcacggcg cgggactcta ctcagtggtg
     8041 gtgcatgctc tcggtgcgcg gccggtagaa gcggtccacg agcttcctgt tgggccgcgc
     8101 gaccaccggc tccgtgttcg gggcattgat acgtacgcag ccgaggaagt cgtcacaggc
     8161 cttgccgccg ttcaggacgt cgtcgacccg ctgcttcaac agggacggct tccagccgtt
     8221 gaagaagtcg ccgtgcgctg tcagcacgga gccgctggcg agcagcaccc cccggccgtc
     8281 atgcatcggg tagacgacgt tgagctggag ctgcggcacc acgatcggat ggctctttgg
     8341 acagcgctgc ggcccgaact ccatcccgcc ttcgcggttg taggccatat gcgaccggtg
     8401 gtccgggctg tcgagccgct gaccgtccca gcagtccgga aacgtcaccc gaagcgttac
     8461 gaacttcgac ccgcacgagg gaggtctggc ggcgccctct ccctggtcgt cctggcaggt
     8521 ccagcggacg atgccgcgct gccaccgctt ggccttgtgg ctgcccgcca cgatcctgag
     8581 cccgggcggg aacggcagga cacgcccgta ccggaagaag tcctggtagt acgcctgcac
     8641 ctgaaccggc ttcagcgcac gcccctgccg gaacagagtc ggcgtccagt agccggagcg
     8701 gtcgccggcg ggcaggcagt tgcccgtgtt gcgccggagc gagcgcagcg tggtcgacgc
     8761 gttcgtcgac gcgttcccga agaagtcgtg catgtgcgag gcgcccggca tgccgtgaaa
     8821 gacgatcggg tcgtttgcgg cccggtggct cagcgagcaa tcggccacga atattccgtc
     8881 ggcgcgggcc gacgccggcg cgcctgcgga gagcacgatt gccaggagtc ccagggcagc
     8941 cgaaccggcg acgggacggc ggccgcatcg cgggaaaagc ggcaaaggca tgctgatctc
     9001 cagggatcgg gcgggtacga ggactcgcac cgtaattcca ctgcgggcgc gagatccctc
     9061 gcactgcggc catgcatcgg atcgacgaac cgaacttgcg agcgctgcga catatccgcg
     9121 ctgagacatg gactgctatc cctatccctc gtgaaggtgt tggttacagg cgccagcggc
     9181 atgctgggca gcgacgtcgt gcgcgctgct cggttcgtga accacgaggt cgtgcccgtc
     9241 ggccaccagg agctggagat caccgacgcc gaggcggtgc gccggctgtt cgagtcggag
     9301 caaccgcaag cggtcgtcaa ctgcgccgcc tacaccgacg tggacggcgc cgaggacgac
     9361 ctcgagggcg ccatggcagt gaatggcgac ggcgcgcgga acgtggccgc ggccgcgcag
     9421 gcggtcggcg cgcaggtggt gtacccgtcc accgactacg tgttcgacgg ctcgaagtcg
     9481 gagccgtacc tggagacgga tgagccgcgc ccgctctctg tgtacgggca gacgaagctc
     9541 gcgggcgagc acgagacgat cacctccaat ccggcccact acgtcgtccg cagctcgtgg
     9601 acgttcggcg tgaacggccg caacttcgtg gacacgatgc tcatgctcgg cgccgaccac
     9661 ggcgaggtgc tcgtggtgcg cgaccaggtc ggcagcccga cgtggaccgg tcatctctcc
     9721 gacgcgatcg ttcgccttct cgacggcgag gcgtacggga tccaccacat cgcgagcgac
     9781 ggcgagtgct cctggtacga attcgcgatc gcgatcttcg agcaggcagg cgtagacagc
     9841 cgcgtgctgt cgtgtaccac agacgagttc gggcgccgcg cgccgaggcc gtcgtactcg
     9901 gtgctccgca gcgcgcgcga cgacacggtg ttcctccccc actggcgcga agggctcgcc
     9961 agctacctcg ccgagagggc actggccgcg tgaggctgct ggtcaccggc gctgccggtt
    10021 tcatcggctc gacgttcgtg cgggtggtgc tcgaggagcg ccccgacgcc gaggtcgtcg
    10081 tgctcgacaa gctcacctac gcgggccggc gagagaacct gcaggaggtg gaggaccgga
    10141 tccgcttcgt gcagggcgcg atcgaggacg aagaggtcgt gctcgagacg atggagggcg
    10201 ccgacgcggt cgtgaacttc gccgccgagt cgcatgtcga ccgctcgatc tcggaccagt
    10261 acgcgttcgt gcgcacccac gtcatcggga cgagcgtgct gctcgacgcc gcccgccagc
    10321 acggcgtgag ccgcttcctc caggtctcga cggacgaggt gtacggttcg atcgaagaag
    10381 gctcgttcac ggagacctcg ccgctcaatc cctcgagccc gtacagcgcg accaaggcgg
    10441 ccggcgacct gctcgtgagc gcgcatgtgc atacgcacgg catcgaggcg gtgatctgcc
    10501 ggggctccaa caactacggc ccgcgtcagt accccgagaa gctgatcccg ctgagcgtgc
    10561 tgaacgccgt ggcgggggat cacctgccgg tgtatggcga cgggaggcag gtgcgcaact
    10621 ggctgtatgt ggaggacttc gcgcgtgcga tcgacacggt gctgcgcaac ggcacgccgg
    10681 gcgaggccta caacgtgggc ggcccggacg aggaggagaa catcgacgtg gtgcgtcgca
    10741 tcctcgacct gaccggccgc gacgagtccc tgatcgagca cgtgaccgac cggccgggcc
    10801 atgaccgccg ctactcgctc tcgagcgaca aggtgcgcgc cctggggtgg gagtcgcgcg
    10861 tgcgcttcga ggaggggctc gagcggaccg tcgagtggta ccgcgacaac gagtggtggt
    10921 gggcgccgat ccggagcggc gagtaccgcg actactacga gcgccagtac ggccgcagtc
    10981 ttggctgagc agtctccggc actcgagctc aaaggcctca cgaagcgcta cgacgacggc
    11041 accgaggccc tcgtggactt cgacctcgtg gtgccggacg gcgcgttctt cggcctgctc
    11101 ggcccgaacg gcgccggcaa gaccacgctg atcagcgcgg tctgcaacct gatcaggatc
    11161 accagcggcg acgtgctcgt gttcggcagg ccggccgccg atctcaccgc gcgcagctgg
    11221 gtcgggctcg cggagcagga catcaacctc gaccgctttc tcaccgtgga ggagacgctg
    11281 atctaccacg gcggctggtt cgggatgaac cgcgacgagg ccgaggcgcg ggcggccgag
    11341 atgatcgacg tgttcgatct gcgcgcgaag gcggacacgc gcacgccaaa gctgtcgggc
    11401 ggcatgcgcc gccggctcct gctcgcccgc gcgctgatgc accgcccgcg cctcgtgatc
    11461 ctcgacgagc ccaccgcggg cgtggacttc gagctccgcc acgagctctg gcgctacatc
    11521 cgcacgctgc acggagaggg cacaacgatc ctcctcacca cgcactacct cgaggaggcc
    11581 gaggagctgt gcgaggagat cgcgctgatc cgcggcggca ggttgatcgc gcgcgacacc
    11641 tccgatggcc tgcgcgccca ctacggcgcc gcgagtctcg ccgatgtgta cgtgaaggcg
    11701 atgggcgtat gaccggggcc cgcggactca tgtggctctc gcggcgcgag gtgctgcgcg
    11761 tctcgaagct gtggactcag accgtgcttg cgccggtgat cagcagcatg ctgttcatcc
    11821 tcgtcttcgg gctcgcgctc ggcgggcgct tcaagcaggt cgagggcttc gactaccgcg
    11881 aattcatcgt gcccggtctg atcacgatgg cgatggtgca ggcggcgtac aacaacaacg
    11941 cctcgtcgat cttccaggcg cgctccgacc gctacatcaa cgacgtgctc tcggctccca
    12001 tgcacgcctg gcagatgaac gtcggcttta acgtcggcgg cttcgtgcgc gcgctctgga
    12061 tcggcgtgtc gctgctggtg ctctcgctgc cgctgacggg cgtgccggtg cgcgagccgc
    12121 tcgtgctgat cgtggccgtg ctactcgcgc tggtgctgtt ctccgcgttg ggcacggtcg
    12181 tgggcatctt cgcggagacc ttcgaccacc acatgttcgt ctcgaacgtg gtgatcctgc
    12241 cgctcacgtt cgtcggcggt gtcttctact ccgtcgaccg gctcggctca ccgtggcacg
    12301 agctgtcgca tctgaacccg gtgttctacc tcgtgaacgc cgtgcgctac ggctttctcg
    12361 gcaccgccga cgtgagcgta tggcttgcgc tcggcgtgac ggccgcgctg gcgatcccgg
    12421 cgtacgcctg gtcgcactgg ctgttcgtca ccggccggaa gctgaaggac tagcgctcta
    12481 gctcgcgcga ggcctcgctg gcccacgcga ccgactggtt gtaggcgtcc tgcaccgaca
    12541 cgccgacgcg gggcgcgagc tccacgagct tctggaagtc gccgctctcg aacgcgagta
    12601 cggcaccgag cacgcgcccc ttcgggccct cctgctctga gagcgcgatc ttgagctcgt
    12661 ccgagagcgg cagcgaagcg aggatcttct ccatcggcgc gtccatcagg gcctcgacca
    12721 ccgagaacat gccgaccgtg aagtgctcct cgtgctcgcg ctgcacctgg ctcggcgaga
    12781 gcagctcaca cattcgcgcg cggatgagcg cggtgacgat cagctcgtgc ggcttgtcgt
    12841 caacgcccgc catcacgagc agcgttgacc agcgcttgat cgcctggagg ccgagcagca
    12901 ccatcgcgtg gcgcacggat ccgaccttct gcggcagcga gaagaacgcc gagttgatgt
    12961 agcgcaacag cttgaagctg agccccacgt cgcggctgat cagcttctcg acctcgtcga
    13021 agtccgcgtc gtccgagttg agctgcgcga tcagcttgag ctgcgcgagc ctgttcgagg
    13081 ggatcgcgcg ctggcgcagg accttcggct tgcagaagaa gtcaccctgg aagtagtcga
    13141 tcccgaggag cttgcaagcc tcgaactcct cgtagctctc gaccttctcc gccacgagct
    13201 tctttccgta tggcaggagc agccgcagct ggccttccag cgccgcggga tcgtgctcgc
    13261 gcacgtcgag cttcacgatg tccaccatct cgaggagcgg cttgatcgag tcgctgaacg
    13321 tgaagtcgtc gagcgcgatc gtgtagccct ggcgtttcaa gcgctgcacg gcctcgacga
    13381 gctggtcgtc gacctccacg tcctcgagga tctcgagcat catgcggtcc ggcgcgagtg
    13441 gcggcatctg cgattcgagg atgaaccggc gagtcacatt gatcgacgcg aggcggttgt
    13501 cgacgatcgt ctcgaggcca tctcggtgaa ggcgttgccg atcacccggg cggtcgccgc
    13561 ctcgccgtcg gcgacgagcg ccgaattgcc gctgccgtcg cggaacagca gctcgtacgc
    13621 gaacacctcc acctgccggt cgtagatcgg ctggcgcgcg atgaagatct cgttcgccgc
    13681 gctcggctcg ggcacgggct gtggcagggc gttcacctcg ccagtgtgat cgctcggagg
    13741 ccgcgtatct tgaggccgtg cccgagctcg ccgacatcga gcgccaggtc accgagtgcc
    13801 ggcgctgccc gcggctcgtg gagtggcgcg agaaggtggc ccgcgacaag cgccgcgcgt
    13861 tcgccggcga ggactactgg gggcgtcctg tcccagggtt cggtgacccc gccgcgcggg
    13921 tctacgtgct cggtctggca ccggctgcgc acggcggcaa ccgcaccgga cgcgtgttca
    13981 ccggggaccg ctccggcgac ttcctgttcg agtcgctgca tcggacgggc ttcgccaacc
    14041 agccccggtc agtgagccgc gacgacgggc tcgagctgcg cggcgcgttc gtcgcggcgt
    14101 cggtgcgctg cgcgcccccg gccaacaagc cgctgccgag cgagcgcgac aactgcctgc
    14161 cgtatgcggc cgccgaactc gaagcgatgg cgagcgtgcg cgtgatcgtc tgtctcggct
    14221 catacgcgtg ggactgcgcc tgccgattgc tcggcatccg ccccaagccg aagttcggcc
    14281 acctggccat gttcgagcac gagcgcgcgg tgctgctcgg ctgcttccac ccgagccagc
    14341 agaacacgtt caccggcaag ctgacgccgg cgatgatgga cgaggtgttc gcgagagccc
    14401 gggagctctc ggacgctttc tagccggcga ggatgaagcc gatggccggc cacgcagtga
    14461 cgcggccggc cgtgggcttc accggagtcc ggcttttttt attcgtacgc caacgccttg
    14521 agcacgtcga gcttcgccgc gcgcctggcc ggcgtgatcg ccgcgaccat cccggcgagc
    14581 gccgccagga cgagcaggat gatcagggtc gcgatcggga acgagagcga gaagccctcg
    14641 gacgcgagcg gccgggagac gatgatcgcg aagaagaccc cgagcaccgt tccgagcgtc
    14701 gccccgatga gcgcggtaat gatcgactcg tagcggacga tcctcttgac ctgcttgcgc
    14761 gagccgccga ccgcgcggat catgccgatc tcgcgcgtcc gctcgtagat cgcgagcacg
    14821 agcgtgttca cgatcccgaa cagcgacacg atgacggcca gcgagagcag cacgtagaag
    14881 aaggacagga tcccgttgac ccacttggtc tgggtgtcct tgaagccgtc cttcgtcacc
    14941 acctcggccg tcgggaactg gctcgagacc accttcgttg cctgcgccct ggcctgatcg
    15001 gcgttagctc cctgctgcag gcccacgaag atgtactggg cctgcttctg gccgaactcc
    15061 cggcccatca gcgagttggt gatcaccatg tcgccgaggg cgtggacgtt gttcttgtag
    15121 gtggccttga tcgtgacggg cacttccttg cccgactgtg tctgcagcgt cacctggtcg
    15181 ccgatcttct tgttgtggct gtcggcccag gattcgctcg cgagcgcacc cgagtcggtc
    15241 agcgagctgg cgatcgcatc tgagcccttc ttccactcca cgtggaacag cggagtgaac
    15301 gtggcggggt cgataccgct gagcccgtcg aggttcccga tgcccttcgc gtgtgccgcg
    15361 gagtagcgaa ccggcgagac cgagctcacg ccatcgacct tcgcgacggc ctggccggtc
    15421 gactcgggga tgagcgacca accgtccttg ttctcgacca cgagtccggc ggtgacgctc
    15481 ttgccgatcg cgtcgtcgat cgagtggcgg atgccggcgg cgaagatggt cacgaagacc
    15541 accagcgcca agccgatcat gagagcggcg gccgtgccgg ccgtgcgacc ggggttgcgg
    15601 acgctgttct cgcgggcgat ctgaccggtc acgccgaaac tgcgctcgag cggcgagccg
    15661 atcaccgttg acagcggctt caccagccgt gagctcacga gcgcgactcc aaggaagagc
    15721 agcgctgcgc caccgcccac cgggcccagc gcggcgccgc ccgactggcc tccgaagagg
    15781 ccgaaggcga gaagcgcgat gccgaggacg gagatgccga tcgcgagcgg ggtcttcatg
    15841 cgcgcgagac ggccgccgcg gatctccgca ccctcgcgga cgccctcgat cggcgagatg
    15901 cgggtcgcac gcaccgccgg cccgaggctg gcgacaagcg tgaccagcgt gccgaccagc
    15961 agcgatacga cgatggtgcg tgtctcgacc acggtgccgc ccgagggcag ctccgcgccg
    16021 aaggccttga acagcgcctt cagcgccggc gcgaggccga ggccggccag caggccgatc
    16081 accgaggcgg tgaagcccac gatgagcgcc tcaccggtga cggcgttcag cacctggcgc
    16141 cgcgacgcgc cgagcgtgcg cagcatcgag agctcgcgcg tgcgctgggc gatcgtgatc
    16201 gagaacgcgt tgaagatcat gaacgcgccc acgaacagcg caacgcccgc gaacacgagc
    16261 agcaggacct tgatgaagtt gaagccgtcc ttgacgtcct gcgagtcctg gcgggcctgc
    16321 tccgtgccgg tccgcacagt gaccgtgttc ggcaggtcgg cgcgcacgcg ggcggcgagc
    16381 gcgtccgcgc tcacgcccgg agcggcctgg atgtccaccg agtcgaagcg gccgaccttg
    16441 tcgagcgcct tctgcgcctc gggcgtggtc atcaccgcga tgcccgcgcc ggcgaccgag
    16501 ttgaggttgc cgaacttggc gatgccgacg agcgtgtagg tccttgcggc cgagtggccg
    16561 aggaccttca ccttgtcgcc gaccttccag ccctcgtcgg cggcggtgtg cttgtcgagc
    16621 gccacctcgc cggggccggt cggtgcgtgg ccgctcacgt agttgaaggc gttgaacggc
    16681 tcgggctcga cgttcgtcac gaagttcggc gtggtgttgc tgccgacctt ctcgccgtcc
    16741 ttgccgatga tgctggcctg gtcgaaaacg cctccgacgg ccttctgcac gcccggcacc
    16801 tgctgcgtct gcttgaggat cgctgcgttg aacgccggcg ggttctggcc ctgattggtg
    16861 tcgttgaacg ccttgtgcgg cgtcaccgca acgtcgatgc ccttcgatac ctccgtgaag
    16921 acgttgtcga acgtcttcga gatcgtgtcc gtgaggatgt aggtgcccga gatcagagct
    16981 acgccgagca ccaccgcaaa cgcggtcagg aaagtgcgga gcttcctttc ggtaagcccc
    17041 ttcagcgagg tggcgagcat gtcacgcgac ctcgcgcatc aggtcgacga cctcgctgcg
    17101 gtcgccggcc gggccctcgt tcacgacctt gccgtccttc agtaccacga tgcggtccgc
    17161 caccgaggcg gcggtcggat cgtgggtcac catgaccacg gtctggccgt agtcgtcgac
    17221 tgcgcggcga agcatcgcga ggacctcctc gccggccacc gagtcgaggt tgccggtcgg
    17281 ctcgtccgcg aacaccacgg cgggacgcgt gagcagggcg cgggcgacgg ccacgcgctg
    17341 ctgctggcca cccgaaagct ccgacggctt gtgcttgcgg cggtcggaca ggcccactgc
    17401 ctcgatcagc tcgtcgagcc aggccggatc gggcttctcg ccggcgagct tcatcggcag
    17461 cgtgatgttc tcctccgccg tgaggatcgg aagcaggttg aagaactgga agataaagcc
    17521 gaacttgtcg cgccgcagcc ccgtcagggc ggcgtcgtcg agggaggtca gcgacacccc
    17581 ctccaccagg acgtcgccgc cggtggggcg gtcgaggccg gcgaggatgt gcatgagcgt
    17641 cgacttgccg gagccc
//
LOCUS       DASVXS010000207        29629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_198439, whole genome shotgun sequence.
ACCESSION   DASVXS010000207 DASVXS010000000
VERSION     DASVXS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 29629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29629
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198439"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF752_11480"
     CDS             complement(<1..481)
                     /locus_tag="VF752_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8856211.1"
                     /translation="MTDREQLLDELRPVAFAIAYRMLGSVSEAEDVVQEALLRVHRAL
                     DQGEQIASPRAFVATVTTRLAINELRSARARRERYVGEWLPEPIITDGEDDPARHAET
                     ADSLSLAMLVLLESLSPEQRAVLLLHDVFDYGYPEIAPIVGKSEDNVRQLATRARRHV
                     "
     gene            complement(478..1410)
                     /locus_tag="VF752_11485"
     CDS             complement(478..1410)
                     /locus_tag="VF752_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8856212.1"
                     /translation="MRVFVAGATGAIGKQLVPRLVAAGHEVHGMTRSESNQALLGELG
                     AVPVVADALDPEQVAEAVGRARPDVIVHELTAIGAVDMRHFDRDFALTNRLRTEGTDH
                     LLSAGQAIGVRRFVAQSYGAWWYVRTGGPVKSEEDPLDPTPAREMRESWAAIRHLEQA
                     VAGADWTEGIVLRYGAFYGPGTSMAPGGELPEMIRKRKFPLVGNGGGVWSFIHVADAA
                     EATLAAVEHGSRGIYNVVDDDPAPVAEWLPALAQTLGAKKPVRVPRFIGRLFAGEVGV
                     VMMTDARGASNAKAKRELGWRPAHPSWRQGFLAA"
     gene            complement(1502..1574)
                     /locus_tag="VF752_11490"
     tRNA            complement(1502..1574)
                     /locus_tag="VF752_11490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1539..1541),aa:Ile,seq:cat)
     gene            1741..3153
                     /locus_tag="VF752_11495"
     CDS             1741..3153
                     /locus_tag="VF752_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8856213.1"
                     /translation="MTTVLDQAAVETLSVNFRGEVITPADERFDEARAIFNGMINKRP
                     ALIAQCNGVADVISALGFARERGLSVAVRAGGHSVPGHCLCGDEGIVIDLSGMKGIWV
                     DPVAQTARVQTGVLWGELDRETQQFGLAVTGGRISTTGVAGLTLGSGSGWFERAHGLT
                     SDSLIAADMVTADGNLVRASSTENPDLFWGLRGGSGNFGIVTSFEFRLQREGPLVYGG
                     LAGWPRAQAPQVLRAFRDFMKSAPNEIGFLAALITAPPEPFIPEELRGQPIVALAGCC
                     AGDPEKAAEAMRPLLEEMPPALNMLAPMPYVVVQQLLDAGNPKGLQGYFKADFVPELS
                     DEAIETIVDQASRITSPFTALLLEPLGGAINDVPEEDSALSRPRADFVYHALVMWEDP
                     AENDRQIAWTRGFADAVAKYTVPGVYLNFLIDEDEEARVRSTYGPEKYDRLVALKDKY
                     DPDNVFRHNYNIKPSGAAAR"
     gene            complement(3160..3348)
                     /locus_tag="VF752_11500"
     CDS             complement(3160..3348)
                     /locus_tag="VF752_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856214.1"
                     /translation="MDREVPNGLFARPVLHYVAMLTAARDFGLSTPDIDAIVHRFPDP
                     APSDELADALADAILSRA"
     gene            3503..5161
                     /locus_tag="VF752_11505"
     CDS             3503..5161
                     /locus_tag="VF752_11505"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX8856215.1"
                     /translation="MTTSVAARRAARALPPWPADRPLRSWQERALATVARFEGRSFLA
                     SAAPAAGKTTFGLKLAHEMLSCGQVARVVVVAPTTHICRQWALDAARYGIDIEPNRPN
                     SARREPRDRHGVAITYQTVAAGPGTHGAACAARPTLLIADEPHHMGEQAAWGTRAQAA
                     FEGATFRLLLSGTPFRSDSTAIPWVTYDEDGVSRADFTYGYTDALLDGVCRPITFQLY
                     DGDMEWVSGGRKRHADFSMVLPRDEAARRLRTALDPDGDWLGEVLRDADARLSAVRAA
                     RHPEAGGLVVAADKEHAARIAMRLARISGEAPEIVTSDEPDSSARISAFSGSSRRWLV
                     SVLMVSEGVDIPRLRVGVYATAARTELFFRQVIGRFVRRTPVPRAQMSYLFLAADPRL
                     RELAARVEEERNHALELRPELEEEHEALERVVGEGAMFEALSSSARADDVITSSMRPG
                     DELQLFYDAAPVKAVPAPAPPPPAPPCISVRPSGPEPAYARRERLRAERNRLVSDISR
                     RTGEPHRQIHGRMNRATGAASVANATIAQLEKANELLVRELRRG"
     gene            5224..8109
                     /locus_tag="VF752_11510"
     CDS             5224..8109
                     /locus_tag="VF752_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8856216.1"
                     /translation="MERNASREGGGGEMATSQPPEGFDPAVTRVLDELLQGRYFVGDA
                     KGRVTRWSAGAETLTGWSQKEVGGRSAFSRPLAWSSEEAEKRLGELSAGGESALQRLG
                     MTILCRDGRELPVEVTVVPVALSLGYEFTDMVSDLADHGPGSRGIEQLNAQHRAAVAA
                     IASSVTGATAWPEHPAGMLVTFRVTGDPVVPSDRSARAAARALRIELEDAVPTAPAED
                     GDTAAPISEQEGAALQKLSEAQAEAELLRAQVADLRERVREAEEADALESAERERLEA
                     ALSAATSRADEAARRAVDAAEQAERVEEKLATAREGLVETERRALDLAHELEDARETR
                     TKLEAEIESLRGDAAAAERLSGEHEELSSTLESAQSELDRLRAELDAARAAEAKLQAE
                     HEQAVAERDELVEHARAEAAETAAERDGLASELGKAQAEVDAVRTELATVIGERDSAL
                     DELRSQAADGERTAEELAATAAERDKLRAELDATLDERDEARAAVAAASDEREELRAS
                     LEDARGALQSTSESDALDAASRDRDDARSSLSRAERELERLRAALDGAKRERDDVRRT
                     LQSTAGEEGRTRAALEAITHERDEARDQLATASTERDKALENASTAAKELEKLRAELE
                     SVAAERDDTQRTAAAAVSDREELRAALQGAVSERDEARATLEALTREAKDVSEARRDE
                     AQAKLERATAALAEAQAQLDATTSERDELRADLAQAVADRDDAVVALDTAARDRDSAQ
                     AALERAVSRAHHEAEASRAGLVDLAASADELRARLADERDRRSGLERKLAEDRRRLAE
                     ERRRAAELELRVAELEAERENGIALPPIHSEVAPEPLPEDPSTVRTGLDDVPTPRAYI
                     DFLGHFSALNAAFCTLVGYSEREFRMAAWPPVVDAANREKLRALTRRMAEGEIESAPV
                     RTQYMHAEGLLVPISGTITVERDVSGRPLHMVLDADAPERMPIGFRR"
     gene            8123..8593
                     /locus_tag="VF752_11515"
     CDS             8123..8593
                     /locus_tag="VF752_11515"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8856217.1"
                     /translation="MASHPGVGDPAPDFELEGTDGAFRLSDQRGKRVVLLFYPGDETP
                     VCTKQFCSYRDRAEELENLDAVVVGISSGSLDSHNSFREHHGLTVPLLADTDREVANA
                     YGAAAKLVGTRRAVIVIDENGIVRERHVHALGVTYQTVDDIRAMLDKLPAPAAG"
     gene            complement(8590..9561)
                     /locus_tag="VF752_11520"
     CDS             complement(8590..9561)
                     /locus_tag="VF752_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856218.1"
                     /translation="MRTKLACAALVVFAAATTAGCGGGGGSSGDTSSQPTVAKPADFP
                     KPDRGSLVEMRQELASGPVLAPAQAVLEPGRNRFAFALFDRARKQITDAAVAVYVQPA
                     SGGRVLGPFPAHDESLAVAAPYRSQTVANDPDAAQSVYVANIRLPKAGNYNLLGVARL
                     DDRLVAADPAAPTRALARSPVPDVGQKAIPVDTPTVASAGSIEKVDTRQPHDDMHDVN
                     LKDVLGKQPVVLLFATPALCQSRVCGPVTDITEQLKAKYGKQAAFIHMEIYKDNDVSK
                     GFRPQVLAWHLPTEPWVFTIKADGTIAARIEGAFSASELDAAIKKAL"
     gene            9656..10378
                     /locus_tag="VF752_11525"
     CDS             9656..10378
                     /locus_tag="VF752_11525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8856219.1"
                     /translation="MEGRAALITGGSSGIGLAIARALGQEGYGLTVSARRPEKLEEAV
                     KGLQDEGLDAQAVQANMTDEDQIKNVVERHRDKFGRLDVLINNAGVGIGGPVEEAETK
                     KVDMQLDVNLRAVYLTARECIPMLKAAGGEHGKALIVNTASIAGKHGQGWLAMYSATK
                     AGVVGFSEALQQELDQSGVQVTAFCPGFVDTPMTDWTKEQVPAEEMIRPEDIAEAVLF
                     LLKTSPACLVPEIQFVRPGDRP"
     gene            complement(10375..10824)
                     /locus_tag="VF752_11530"
     CDS             complement(10375..10824)
                     /locus_tag="VF752_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856220.1"
                     /translation="MGKRDRKRRSPARDLRTAVECLPEKTRTAMLEGVREGGIIAGAY
                     TDRDGGVCPMLAAHRRGGRTAMVSFARAWDRYTGAIGSPRRATRRELRTLEAMLEGSL
                     GVPTGDLARVVEEIKAARRAEFEREAAAERDRPLSLEWPQRDREPVA"
     gene            10914..11945
                     /locus_tag="VF752_11535"
     CDS             10914..11945
                     /locus_tag="VF752_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX8856221.1"
                     /translation="MRLGLQVGYWGLGLTPEQQLEMVKSAESAGFDSVWSAEAYGSDA
                     ATVLAWIAAQTEKIKIGSAIFQIPGRSPGLTAMTAATLDNLSDGRFLLGIGSSGPQVA
                     EGWHGQRFPKQIQRTREYIQIVRKALSRDRLEYRGEVYELPLPDGPGKALKLTIAPVQ
                     EHIPIYLAAIGPKNTQLAGEVADGWLPTFFSPEHVGEFRGLLEEGAQRSGRDLNGFDI
                     APNVNLCIDDDVEKARDAMRPLLALYVGGMGSREQNFYNKLMQRYGFEAAAKEVQDLY
                     LDGKKDEAAAAIPAELIDKTSLVGSADQVKERLELYRDADVGTLIVTPLAWSLEDRNR
                     MLKTLAELV"
     gene            11891..13174
                     /locus_tag="VF752_11540"
     CDS             11891..13174
                     /locus_tag="VF752_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX8856222.1"
                     /translation="MVARRPQPHAQDARRAGLTASAAKPRLSLLLAAFGDPGHAFPAI
                     ALGKELAARGHDVCLQTWRKWSEHVEREGMRFEPAPEYEVFPREGSALKPYQAAVRAA
                     EETVPLIRELDPQAVVADILTTAAALAAGMESRPWATLVPHVLPAGEPGFPLYSVGAR
                     YPRTALGRALWRLLDPIEDIGARQGRRELNEARRRVGLPPFEHLHGGTSRQLALIATF
                     PQLEYPRRRWEPWMRVTGPLMWEQPYGDTDLPPGDDPLVLVAPSTSQDPKQRMLRAAL
                     EGLAGEPVRVLGTTNRREPAEQLEIPPNARLVDWVSYSRTMPHCDAVVCHGGHGTVVR
                     ALAYGVPVVACPAAGDMAENAARIAWSGVGVALPRRLASARGVRAAVRRLLGDESFAR
                     RAAELAGWARSHDGAAIAADEVERFASGRPPSGGA"
     gene            complement(13143..14207)
                     /locus_tag="VF752_11545"
     CDS             complement(13143..14207)
                     /locus_tag="VF752_11545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8856223.1"
                     /translation="MSLLSLRQLFARQADPYVGSDIVIANRIGGILWLVMGALTMVAA
                     PLAPPTARLGWPGWLVAAAIVAGSIAGGRALVRGKQVSHETLFKLSFLGVALVALLQW
                     LAGSRHDSYHELYLPWALLTGSLNPPRRLGAFMLFLAAASGAFLAYDGWDTRAAGEMA
                     SQLLIWTAIAIVASIYATSVRSQRAGLRQEGEDARRLARVDALTGLGNRRALEESLAA
                     EVARCERTGSTLSVLVIDVNGFKGINDRHGHLEGDRCLRRVADAVRSSLREMDSGFRW
                     GGDEFVVLLPETDAVSAHAAADRLREGMVVTCREPTGESLSIGCGVAQLEDGMSANEL
                     LTMADLALVASKPRRSAGVR"
     gene            complement(14204..15886)
                     /locus_tag="VF752_11550"
     CDS             complement(14204..15886)
                     /locus_tag="VF752_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8856224.1"
                     /translation="MDALPSPVGLPERSLDSAALPPALLPSSVAIIRALGFSYLGGAA
                     LGVALALPGTLQVSRSLLLALAVLASVLGATLVLFAKRLPTWVLNVGMAAGVVLIGLV
                     VWLDDPATSDLSLLYVWSGLNGFFFLSRRGAIGQIVLIAVSYTALLALDAGDGFPLDR
                     LLLTLVVVCVAGGLVDFLRSRVERLVAHISKAHQTDALTGSLNAAGFDDLIERELERA
                     QRSGSRMSIVVCDIDRFKDFNEEHGHRRGEDAIRRVAKVLATRKRRIDTLARLGGEEF
                     ALVVPDTDEHGAYVLADRVRRAVRDSFARSSGLTASFGVASFPRHGGSSEELMLNAGR
                     AVSAAKQLGRDRTVIFNAEVSANIRAASVRQQAQSEEHLAAVLVLAETLDIRDSGTST
                     HSQTVGRYAQQIARELGLPEETVERVHLAGLLHDIGKIGISDSILQKPGKLDDHEWAE
                     MRKHPELGARILDGANLEDISAWVLAHHERPDGGGYPLGLGTDEIPLEARIIAVADSY
                     EAMRADRVYKPGMGLEAAVAELRRCAGSQFDPQVVEAFLRALERERDEAVAA"
     gene            complement(15962..16033)
                     /locus_tag="VF752_11555"
     tRNA            complement(15962..16033)
                     /locus_tag="VF752_11555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15999..16001),aa:Asn,seq:gtt)
     gene            complement(16109..17104)
                     /locus_tag="VF752_11560"
     CDS             complement(16109..17104)
                     /locus_tag="VF752_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8856225.1"
                     /translation="MTAKDSSFSAALDDPERDYEQEESQSEEPEEVADLYEEGGVVTS
                     GLPAAPPEGGHEDSVRLYLKRIGRVPLLTREDEVRLARRAERNDMTAKNALVEANLRL
                     VVSIAKRYTGRGLTLLDLIQEGNIGLIRAVEKFDWRRGFKFSTYATWWIRQAITRALA
                     DQSRTIRIPVHMVERMNRVARVRRALIQKNGREPTPEEIGKELEMTPQRVEEILKFGQ
                     EPVSLEAPVGSEDGDAQLADFIADQATDRPLEAVANRLREADLQAALETLPWRERRVL
                     ELRYGLVGEGPMTLEQIGSEVGVTRERVRQIESKTLMKLKNADAGRLEGTAAEET"
     gene            complement(17104..18855)
                     /gene="dnaG"
                     /locus_tag="VF752_11565"
     CDS             complement(17104..18855)
                     /gene="dnaG"
                     /locus_tag="VF752_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX8856226.1"
                     /translation="MSLYTRDSVERLKEAVDMVDLVGSRTDLRRVGTRWTGLCPFHDE
                     RTPSFSVNAEHKLYHCFGCGESGDAIRFVEATQGLDFRGALEFLSDRYNVELQLEKED
                     PEAEERRRRRERLLQLVDRTADYYARYLWDSGEAAGARKYLLGRGLNEEVLRTFRVGY
                     SPSAWDKVVSGALKDGFSEQEVQAADLGRRGRGGRMYDRFRERIMFPLADSRGRTLGF
                     GARALGQGQQAKYINTAESDLYHKGKQLFGIDLARASAAKSGRIVVVEGYTDVLALHQ
                     AGVTETVAIMGTALTQEQLSELSRAAGTVFLALDADRSGQAAMLRAARAADEAGIELR
                     VVDMPEGEDPADLITERGAEAFKGLLDSASTVPEFQVRRVLADSDLSTPRGRDRALEQ
                     VRPLIAAIPEKTATRDAVVRYAADRIDVPVHYLETTLSEPTTQARERRDNGARPRFSI
                     DQATRAERAFLAMCLAQGEAGRSYLSQLTDEHLGSEALRRVRDHLADHFDDPLAGLSP
                     DDPEVSAIVTNVAMLAEHEPSSEPALRLGFLQLELRRIERDLRQAGETGDFDRQRRLW
                     SAREDVRERIAHVMGEA"
     gene            complement(18876..19892)
                     /locus_tag="VF752_11570"
     CDS             complement(18876..19892)
                     /locus_tag="VF752_11570"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX8856227.1"
                     /translation="MASQAVQPQQIPLAERQSAFEDAFLSPLAQRSYPARRALPEEDS
                     PVRTPFQRDRDRIVHSKAFRRLKHKTQVFISPEGDHYRTRLTHTLETCMIARTVARAL
                     ALNEDLTEAIGLGHDLGHPPFGHIGEQVLDGCLQRRYGVRFRHNHHSLRMVERLERDG
                     RGLNLTEQVRDGILRHTGPELPGTLEGRIVRLVDRIAYINHDIDDAVRAGILAYEDLP
                     AREIDALGDTGSKRIDTLVRDLVECSDREGDIVQSEQVGEAMARLRSFMFETVYLGPV
                     AQREQPRIERMLTALFDSLAADPPPAATPDAAEAERVTDYLAGMTDRFALRRFAELSL
                     PQGF"
     gene            20231..20716
                     /locus_tag="VF752_11575"
     CDS             20231..20716
                     /locus_tag="VF752_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856228.1"
                     /translation="MDPLRRHIALRAALLAFAAALLVLAVPASPAAANKRASASAAAR
                     IVSLARKELERGVREIPDGSNKAPAIWRYDEATTPHWYGAPWCAYFVSYHREARRGAD
                     RPRRQGTRLCALHPLVGEADRPLDPQAVPRRAHHLPAARRHRRERLLERHADNDRGQL
                     R"
     gene            20858..22159
                     /locus_tag="VF752_11580"
     CDS             20858..22159
                     /locus_tag="VF752_11580"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8856229.1"
                     /translation="MTAYPSKNVGVGQTVDFTSNDSSGDIAKAQWDLDGNGSYEQTGD
                     NVKRRYDKAGSYLVKLKLTDRKHHTATATITITVTSNRAPVAVLDMPTQIEVGKSFKA
                     SATRSSDPDGKIVAWYWDLNGDGDYGADGSSRYFTYDTPGDYYAGLRVKDNDGNVSEV
                     HVPVHVYDHPDPVSKLSCDASSIPSGGTLHCHADDSASPTKIVKHEWDMNDDGSYGTG
                     GNDSSYTYSTSGWYTVHLRVTDVNGRRSELSTDIQVTNRAPSARITLPAKVVVNQPAS
                     FGGGGSSDPDGTLFQWEWDWDGDGFYDALGSSASTTFTSYGQRRIGLRVTDNLGATAV
                     ATTTVNVLAPPVSVPRILTATPLAGTATSFDPALSYDPDGRPLTVFQWDFDGNGTVDR
                     TTTTATPVTKTYSGRGSYTAKLTVTDADGLTGTATVTVSVN"
     gene            complement(22156..22665)
                     /locus_tag="VF752_11585"
     CDS             complement(22156..22665)
                     /locus_tag="VF752_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856230.1"
                     /translation="MRSRLMPVAVLAIAGFATAALVASGGSAQAPGARTLTFKELNRG
                     STFNFVDNAPLSRKRHGFPVSSSPGDLIMFSTRLADQAGKVAGTLEGTCTVVKGSRSF
                     EQSQFICNAVASLTGGTMTLEARAKFSENTTTAAITGGTGAYNGARGTFKSVTRNDRS
                     TDTFSLLAG"
     gene            complement(22748..23173)
                     /locus_tag="VF752_11590"
     CDS             complement(22748..23173)
                     /locus_tag="VF752_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856231.1"
                     /translation="MQSVRQALAVAPRRVRLSGRVLALALAASLALSGVLALALRDVA
                     APVRHRAAFTPTALIGGLPLQQARCQEWIGATPSERGAIVRALAGVVGGPSTSGGVGT
                     TLSTARANALFDRVCASRVAQGFLLYEMYIRAAGFSSYR"
     gene            complement(23149..25107)
                     /locus_tag="VF752_11595"
     CDS             complement(23149..25107)
                     /locus_tag="VF752_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8856232.1"
                     /translation="MAIAVGALTGVSSALAHVERTAYWPDPAPDTSTWPAAGGKVPAA
                     RSIASALDTKARGDTRVVCKSDSLSVAKEEVATARDKGYRFRPTERKRKLSAASAQRL
                     IDVNTALFDKCRYHDIQPAVTASGNNDRVVVMPGTYTEPDSRAVSPFPPECDQYKTTS
                     DHGSGAVSYEYQYHCPNAQALVAVIGRALGTGLEPQSSPTGRPDPHGIPNLGKCVRCN
                     VQMEGSGPTPQSVVVDAGRVASGNHGPIDAKKDVGIKADRADGFVLKNMTVRHAAEHD
                     VYVLETDGYRLQRMNFYYGGEYGALMFASDHGLTDTCDAVGNGDSAVYPGGAPDTGAQ
                     RDDSFYPTRRLNQTITHCDLHHNNLGYSGTMGNATRVVENNFYGNTTGIATDSFFAGG
                     HPGYPQDSAVFERNRIWSNNFNVYAKDSDVESRVPVPIGVGILIAGGNDDQVKGNRIW
                     DNWRRGTMLIAVPDVISCAPSPENGAPPCTPQGIASVSNRNRYFDNVMSRTPSGGKAP
                     NGVDFWWDEFPTNSNNCWYNNQGADGSEASVTSDPPPPPVPGTSIPKFLPQDCSAPTN
                     VGAGDAAKEAMLASCAGALATGNYDPNTCEWFQMPAKPGTAAAKQQTLRNQQIANQMV
                     GETEPPPPFCDLIGGLGGTVICSPFAKR"
     gene            complement(25200..27851)
                     /gene="ppdK"
                     /locus_tag="VF752_11600"
     CDS             complement(25200..27851)
                     /gene="ppdK"
                     /locus_tag="VF752_11600"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX8856233.1"
                     /translation="MTSTAQATKYVYEFSEGSKDMRELLGGKGANVAEMTRILGAERV
                     PAGFTITTEACVAYMNAGREEPEGLEQQVAEALTHLEEQTGKRLGDPDDPLLVSVRSG
                     ARESMPGMMDTVLNLGLNDESVEGLAKVTDNERFAWDSYRRFVQMFGNVCRGVPGEKI
                     EDAIKAQKETAGVKDDTELSVDDLKELVDSFKQLYRDQTSEEFPQDPQEQLRQAIRAV
                     FDSWMGERAVEYRRINRLPDDWGTAVNVQQMVFGNKGDTSCTGVAFSRDEITGAPEPS
                     GDFLLNAQGEDVVSGVRTPRDISELNEVMPETHTELMEILRTLESHYRDMQDTEFTVE
                     EGHLYMLQTRSAKRPAQAAVRFAVDAVDEELLSREEALATIDAAKLDALLHPTFDPNA
                     EFDVLARGVNASPGAAKGAIVFTADDAVEQGDAGKDVILVRPFTEADDVAGFFAARGI
                     LTSEGGKASHAALVARGMGRPCVSGASDLHIDLNAREVHVNGTVLHQGDLIAIDGTSG
                     VVTTDDVELVDPEVDENFEKVLKWSDEIRRLGVRTNADTPEDAQKAREFGAEGIGLCR
                     TEHMFMAADRQPKMRAMIMAEEEQDRRAALDDLLPFQQEDFEGLFEQMRGLPVTIRLL
                     DPPLHEFLPGLEEIAQDVERARIEQTDDLEELERTLERVHQLQETNPMLGTRGVRLGI
                     VHPEIYEMQVRAVIRAAKAVKERCGEAPHLEVMIPLVAYETELRIMRELVEHVFEEEG
                     DDLERSIGTMIELPRACFAADKIAGQADFFSFGTNDLTQTALGFSRDDVEGKFLAEYI
                     REKIVDRSPFETIDKPGVGWLVRLAAWTGREAKAELKLGICGEHGGDPDSIDFFHMAG
                     LDYVSCSPFRVPIARVAAAQAAVAHSS"
     gene            complement(28002..28592)
                     /locus_tag="VF752_11605"
     CDS             complement(28002..28592)
                     /locus_tag="VF752_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8856234.1"
                     /translation="MLEAPSVERVSESDVTRLASVLARAFLDDPVTCWAFDREPTRLR
                     TAARFFELRLRTLIPHELSYTTHDREGAALWALPGKWLVPIGETLRLMVLVRARRLPL
                     VTRGLTMVERRHPREKHFYLAVLGTDPDHQGQGIGSALLRPVLEMSDSEGIPAYLETA
                     KERNLAFYGRHGFEVTERLELPKGPPVWLMWRRPNE"
     gene            complement(28597..29313)
                     /gene="recO"
                     /locus_tag="VF752_11610"
     CDS             complement(28597..29313)
                     /gene="recO"
                     /locus_tag="VF752_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX8856235.1"
                     /translation="MAGLFKTEAVVLRSFRYGEADRILHLYSRERGRIAAMAKGVRRV
                     KSRFGGRLEPLVRVELVCHQGSGELCTITGASTIHPHARLREQAEPMKRAGQACEAVL
                     RLFDSEEPNAPAYNLLCHELALLDADPAAATRAQALAFRLKLLLAAGFVPELGACASC
                     GEREHLGAFSASAGGVVCAGCEAGSFQLDESAHRFLVDALGRSLSDVPDASESALRQA
                     DRAITETLEHHAHVRLARVA"
     gene            29332..29598
                     /locus_tag="VF752_11615"
     CDS             29332..29598
                     /locus_tag="VF752_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX8856236.1"
                     /translation="MADNGRVTVYTTEPCGFCRQAKALLEARGIVYEEVNLAKSPEGR
                     ADLVQRTGQMTFPQILVGGRPIGGFRELVEADRDGSLKTLLAAA"
BASE COUNT         4507 a        10478 c        10315 g         4329 t
ORIGIN      
        1 cgacgtggcg tctggcgcgg gtggcgagct ggcgcacgtt gtcctcgctc ttgccgacga
       61 tcggcgcgat ctccggatac ccgtagtcga acacgtcgtg aagcagcagc acggctcgct
      121 gctcaggcga gaggctctcc aacagcacca gcatcgccag cgacagcgaa tcggctgtct
      181 cagcgtgccg ggccggatca tcctcgccgt cggtgatgat cggctccggc agccactcac
      241 cgacatagcg ttcgcggcgg gcgcgcgcgg agcgcagctc gttgatcgcc agccgggtgg
      301 tcacggtggc gacgaacgcg cgcggcgagg cgatctgctc gccctggtcg agcgcccgat
      361 gcacgcgcag cagcgcctcc tgcaccacgt cctcggcctc ggacacgctg ccgagcatcc
      421 ggtaggcgat cgcgaacgcg accggccgca gctcgtcaag cagttgttcg cggtcggtca
      481 tgccgccagg aacccctgcc gccagctcgg gtgcgccggg cgccacccca gctcgcgctt
      541 ggccttggca ttggaggcac cgcgggcgtc ggtcatcatc accacgccaa cctcaccggc
      601 gaacagccgc ccgatgaacc gcggcacgcg cacgggcttc ttggcgccca gcgtctgcgc
      661 cagcgccggc agccactcgg cgaccggggc ggggtcatcg tcgacaacgt tgtagatacc
      721 gcggctgccg tgctcgaccg ccgccaacgt cgcctccgcg gcgtcggcga cgtggatgaa
      781 agaccacacg ccgccgccgt tgccgaccag cgggaatttg cgcttgcgga tcatctcggg
      841 cagctcgccg ccgggcgcca tggatgtgcc cggcccgtag aacgcgccgt agcgcagcac
      901 gatcccctcc gtccagtcgg cgccggcgac cgcctgctcg aggtgacgga tcgcggccca
      961 gctttcgcgc atctcgcgcg ccggcgttgg gtcgagcggg tcctcctcgc tcttcaccgg
     1021 cccaccggtg cgcacgtacc accacgcgcc gtagctctgc gccacaaacc gccgcacccc
     1081 tatcgcctgt cccgccgaca gcaggtgatc cgtcccctcc gtccgcagac ggttcgtgag
     1141 cgcgaaatcg cgatcgaagt gacgcatgtc caccgcgccg atcgcggtca gctcgtggac
     1201 gatcacatcc ggcctcgccc tgcccacggc ctccgcgacc tgttcgggat cgagcgcgtc
     1261 cgcgacaacc ggcactgcgc ccagctcgcc gagcagcgcc tggtttgact cgctgcgcgt
     1321 catgccatga acctcatgac ccgcggcgac cagacgcggc accagctgct ttccaatcgc
     1381 tcccgtcgct cctgctacga acactctcat cgtcaagtcc ctgatcgtgg attgcctaca
     1441 cccacaagac gagacagcag cccgggctgt gacagcaccg cgcaggtgag cgaggagccg
     1501 atgggccagc caggattcga acctgggacc gacggattat gagtccgctg ctctaaccgc
     1561 tgagctactg gcccggcgaa agcaaaggta gcttgcgcgg ccgtcgggag actgcggctg
     1621 gtcgcggagt taaggcacga accaccggac ttgctccacc cccagaccag ggttagggtc
     1681 gcaggagccc ccacggcctc gttgcccgcg aagccgcacc cgcataggaa ggactcgcgc
     1741 atgaccaccg tgctcgacca agccgccgtt gagacgctgt ccgtcaactt ccgaggcgag
     1801 gtgatcaccc ccgccgacga gcgcttcgac gaggcgcgcg cgatcttcaa cggcatgatc
     1861 aacaagcggc cggcgctgat cgcccagtgc aacggcgtgg ccgacgtgat ctccgccctc
     1921 ggcttcgcgc gcgagcgcgg cctgagcgtc gcggtacgtg cgggcgggca cagcgtgccc
     1981 ggccactgcc tttgcggcga cgagggcatc gtgatcgacc tctccggtat gaaggggatc
     2041 tgggtcgatc ccgtcgcgca aactgcgcgc gtgcagacag gcgtgctctg gggcgagctc
     2101 gatcgcgaga cccagcagtt cggcctggcg gtcaccggcg gccgcatctc gacgaccggc
     2161 gtggccggcc tcacgctcgg cagcggcagc ggctggttcg agcgcgctca cggcctcacc
     2221 tccgacagcc tgatcgcggc cgacatggtc acggccgacg gcaacctcgt gcgcgcgagc
     2281 tcgacggaga accccgacct gttctggggg ctccgcggcg gcagcggcaa cttcggcatc
     2341 gtcacatcgt tcgagttccg cctccagcgg gaagggccgc tcgtctacgg cggcctcgca
     2401 ggatggcctc gcgcgcaggc gccgcaggtg ctgcgcgcgt tccgcgactt catgaagtcc
     2461 gccccgaacg agatcggctt cctcgcggcg ctgatcaccg cgccgcccga gccgttcatt
     2521 ccggaggagt tgcgcggcca gccgatcgtc gcgctcgccg gctgctgcgc cggcgacccg
     2581 gagaaggcgg cggaggcgat gcggccgctc ctcgaggaga tgccgcccgc cctcaacatg
     2641 ctcgcgccga tgccgtacgt ggtcgtgcag cagctgctcg atgccggcaa tccgaagggc
     2701 ctccagggct acttcaaggc cgacttcgtg ccggagctga gcgacgaggc gatcgagacg
     2761 atcgtggacc aagcctcgcg aatcacctcg ccgttcacag ccttgctgct cgagccgctg
     2821 ggcggcgcga tcaacgacgt cccagaggag gacagcgcgc taagccggcc gcgcgccgac
     2881 ttcgtgtacc acgcgctcgt gatgtgggag gaccctgcgg agaacgacag gcagatcgcc
     2941 tggacgaggg ggttcgccga cgcggtcgcg aagtacaccg tcccgggcgt gtacctcaac
     3001 ttcctcatcg acgaggacga ggaggcgcgc gtccgctcga cctacggccc ggagaagtac
     3061 gaccggctcg tcgcgctcaa ggacaagtac gacccggaca acgtgttccg gcacaactac
     3121 aacatcaagc cgagcggagc cgccgcccgc tgaggcggat caagcgcgtg acaggatcgc
     3181 gtcggcgagc gcgtcggcca gctcgtccga cggagcggga tccggaaagc ggtgcacgat
     3241 cgcgtcgatg tcgggcgtgc tcagcccgaa gtcgcgcgcg gccgtgagca tggccacgta
     3301 atggagtacc ggcctcgcga agaggccgtt cggcacctcc cggtccatgc gacggtctgt
     3361 acccgccctg ggcgccgttc tcacctcgaa acggggcccg ctcggcgctt tccgactgcc
     3421 ggcccgccta gccttgcgcg ccgccgccgg gtcaggggct cggccggctc atcccccacc
     3481 cgagacgaaa ggactctccc gagtgacgac ctccgtcgcc gcccggcggg cggcacgcgc
     3541 ccttccgcca tggcccgccg accggccgct gcggtcgtgg caggaacggg cgctcgcgac
     3601 ggtggcccgc ttcgagggcc ggagcttcct cgcctcggcc gcgccggcgg cggggaagac
     3661 caccttcggc ctgaagctcg cgcacgagat gctctcgtgc ggccaggtgg ctcgggtcgt
     3721 ggtggtcgcc ccgaccacgc acatctgccg gcagtgggcg ctcgacgccg cccggtacgg
     3781 gatcgacatc gagccgaaca ggccgaactc cgcaaggcgc gagccgcgcg atcgccacgg
     3841 cgtggcgatc acctaccaga ccgtggccgc tggccctggc acgcacggcg ctgcctgcgc
     3901 cgcccgccct actctgctga tcgcggacga gccgcaccac atgggcgagc aggccgcctg
     3961 gggcacgcgt gcgcaggccg cgttcgaggg ggccacgttc cgcctgctgc tctccggcac
     4021 gccgttccgg tcggactcga ccgccatccc ctgggtcacg tacgacgagg acggcgtcag
     4081 ccgcgccgac ttcacgtacg gctacaccga cgccctgctc gacggcgtct gccgcccgat
     4141 caccttccag ctctatgacg gcgacatgga gtgggtgagc ggaggcagga agcgccacgc
     4201 ggacttcagc atggtgctgc cgcgcgacga ggccgcgcgg cgtctccgca ccgctctcga
     4261 cccggacggc gactggctcg gcgaggtcct gcgcgacgcc gacgcgcgcc tcagcgcggt
     4321 gcgtgcggcc cggcatccgg aggccggcgg cctcgtggtg gcggccgaca aggagcacgc
     4381 cgcgaggatc gccatgcgac tcgcgcgcat cagcggtgag gcgccggaaa tcgtcacgtc
     4441 ggacgagccc gacagctcgg cgcgcatctc cgccttcagc ggctcgagca ggcgctggct
     4501 ggtgtcggtg ctgatggtgt ctgagggcgt cgacatcccg cgcctgcgcg taggcgtcta
     4561 cgcgaccgcg gcgcgcaccg agctcttctt ccgccaggtg atcggccgct tcgtgcgccg
     4621 cacgcccgtc ccccgcgccc agatgagcta cctgttcctc gccgccgacc cgcgcctgcg
     4681 cgagctcgcc gctcgggtcg aagaggagcg aaatcatgcc ctcgagctgc ggccggagct
     4741 cgaagaggag cacgaggctc tcgagcgcgt cgtgggcgag ggcgccatgt tcgaggccct
     4801 ctcctcgagc gcccgcgccg acgacgtgat cacctcgagc atgaggccgg gcgacgagct
     4861 gcagctgttc tacgacgcgg cgccggtgaa ggccgtcccc gctccggcgc cgcctcctcc
     4921 ggctcccccg tgcatatccg tgcggccgtc gggcccggag ccggcgtacg cccgccgcga
     4981 gcgcctgcgc gccgagcgca accggctcgt gtcggacatt tcgcgtcgca ccggcgagcc
     5041 gcaccgccag atccacgggc gcatgaatcg agcgaccgga gccgcgtcgg tggctaatgc
     5101 gacaatcgcc cagctcgaga aggccaacga gctgctcgtc cgcgagcttc gacgcggctg
     5161 attgcgtgtc gagaacggcg caccgcgctc aagtcgcctc tcgccgatgc cgaacgggtt
     5221 cacatggagc gcaacgcctc ccgcgaaggc ggcgggggcg agatggcgac ctcgcagccg
     5281 cctgaggggt ttgaccccgc ggttacgcgc gtcctcgatg agctcctgca ggggcgctac
     5341 ttcgtcggcg acgccaaggg ccgcgtcacg cgctggagcg cgggcgccga gacgctcacc
     5401 ggctggagcc agaaggaggt gggcggccgc tcggctttct cgcgcccgct cgcgtggagc
     5461 agcgaggaag cggagaagcg cctcggcgag ctctcggccg gaggcgagtc cgcgctccag
     5521 cggctcggca tgaccatcct ctgccgcgac gggcgcgagc tgcccgtcga ggtgaccgtc
     5581 gtgccggtgg cgctcagcct cggctacgaa ttcaccgaca tggtgagtga cctggccgat
     5641 cacggcccgg gctcgcgcgg catcgagcag ctgaacgcac agcaccgcgc ggcggtcgcc
     5701 gccatcgcgt cttcggtcac cggcgccacg gcctggcccg agcacccggc cggcatgctc
     5761 gtcacgttcc gcgtgaccgg cgatcccgtc gtgccgtccg accggagcgc ccgcgccgcc
     5821 gcccgcgcgc tgcgcatcga gctcgaggac gcggtgccga ccgcgcccgc cgaggacggc
     5881 gacaccgccg ctccgatctc cgagcaggag ggcgcggcgc tccagaagct gagcgaggca
     5941 caggccgagg cggagctgct ccgcgcccag gtggccgatc tccgtgagcg cgtgcgcgag
     6001 gccgaggagg ccgacgctct cgagtcggcc gagcgtgagc ggctcgaggc cgcgctgagt
     6061 gccgccacgt cgcgcgcgga cgaggccgcc cggcgcgcgg ttgacgcggc cgagcaggcc
     6121 gagagagtcg aggagaagct cgcgacggcc cgcgagggac tggtcgagac ggagcgtcga
     6181 gcgctcgacc tcgcgcacga gctcgaggac gcgcgcgaga cgcggaccaa gctggaggca
     6241 gagatcgagt ctctgcgcgg cgacgcggca gccgccgagc gactgagtgg agagcacgag
     6301 gagctttcgt cgaccctcga gtctgcccaa agcgagctgg accgcctgcg cgccgagctc
     6361 gacgcggcaa gggccgctga ggcgaagctc caggcggagc acgagcaggc cgtcgccgag
     6421 cgcgacgagc tggtcgagca cgcccgcgcc gaggcggccg agacggccgc ggagcgcgat
     6481 ggcctggcgt ccgagctcgg gaaggctcag gcggaagtcg acgccgtgag gacggaactc
     6541 gcgacggtga tcggcgagcg cgactcggca ctcgacgagc ttcgctcgca ggcggccgat
     6601 ggcgagcgca ccgccgagga gctcgccgcg accgcggccg agcgcgacaa gctgcgggcc
     6661 gagctagacg ccactctcga cgaacgcgac gaggcgcgcg cggcggtcgc agcggcaagc
     6721 gacgagcgcg aggagctgcg cgcctcgctc gaggacgcgc gcggcgcgct ccagtcgaca
     6781 agcgagagcg acgcgctcga cgccgccagc cgcgaccggg acgacgctcg ctcatcgctg
     6841 tcgcgagcgg agcgcgagct ggagcggctc cgcgcggcgc tcgacggcgc caagcgcgag
     6901 cgcgacgacg tgcgccgcac gctccaatcg acggccggcg aggaaggccg cacccgcgcc
     6961 gccctcgagg cgatcaccca cgagcgtgac gaggcgcgcg accagctcgc gacggcgagc
     7021 acggagcggg acaaggcgct cgagaacgct tcaaccgcgg ccaaggagct ggagaaactc
     7081 cgcgcggagc tcgaatcggt ggcggcggag cgcgacgaca cccagcgaac cgccgccgcc
     7141 gctgtctccg accgcgagga gttgcgcgcg gcgctccagg gagcggtatc ggagcgcgac
     7201 gaggcccgcg cgacgctcga ggctctcacc cgcgaggcca aggacgtcag cgaggcgcgg
     7261 cgtgacgagg cgcaggcgaa gctcgagcgg gccacggccg cgctcgccga ggcgcaggcg
     7321 cagctcgatg ccaccacgag cgagcgtgac gagctccgcg cggacctggc gcaggcggtg
     7381 gccgaccgcg acgacgcggt ggtcgcgctc gataccgcgg cgcgcgaccg cgattcggca
     7441 caggccgctc tcgagcgcgc cgtctcgcgc gcgcaccacg aggccgaggc ctcgcgcgcc
     7501 ggcctcgtgg acctcgccgc cagcgcggac gagctgcggg cccggctggc cgacgagcgc
     7561 gaccgccgct ccggcctgga acgcaagctc gccgaagacc gccgccgcct cgcggaggag
     7621 cggcgccgcg cggcggagct cgagctgcgc gtcgccgagc tcgaggccga gcgcgaaaac
     7681 ggcatagcgc tgccgccgat ccactcggag gtggcccccg agccgcttcc cgaggacccg
     7741 agcaccgtcc gcacgggcct cgatgacgtg ccgacgccgc gcgcctacat cgacttcctc
     7801 ggccacttca gtgccctgaa cgcagccttc tgcacgctcg tgggctacag cgagcgcgag
     7861 ttccggatgg ccgcctggcc gccggtggtg gacgcggcga atcgcgagaa gctgcgcgcg
     7921 ctgacgcgcc ggatggcgga gggcgagatc gagtccgcgc cggtgcgtac gcagtacatg
     7981 cacgcggagg gattactcgt tccgatcagc ggcacgatca ccgtcgagcg cgacgtctcg
     8041 gggcgcccac tgcacatggt gctcgacgcg gacgcgcccg agcgcatgcc gatcggcttc
     8101 cggcgctgat agcgtccgct ccgtggcgag ccatcccggc gtgggcgatc cggcgccaga
     8161 cttcgagctc gagggcactg acggggcgtt ccggctctcg gatcagcgcg gcaagcgcgt
     8221 ggtgctgctg ttctacccgg gcgacgagac cccagtctgc acgaagcagt tctgctcgta
     8281 ccgcgaccgc gccgaggagc tcgagaacct ggatgccgtt gtcgtgggca tctcgtccgg
     8341 ctcgctcgat tcgcacaaca gctttcggga gcaccacggg ctcacggtgc cgctgctcgc
     8401 ggacaccgac cgcgaggtgg caaacgccta cggcgccgcg gcaaagctgg tcggcacgcg
     8461 ccgcgcggtg atcgtgatcg acgagaacgg catcgtccgc gagcgccacg tgcacgccct
     8521 gggcgtgacc taccagacgg tcgacgacat ccgggcaatg ctcgacaagc tgccggcgcc
     8581 ggcggcgggc taaagcgcct tcttgatcgc ggcgtccagc tccgaggcgc tgaaggcgcc
     8641 ctcgatgcgg gccgcgatgg tcccgtccgc cttgatcgtg aagacccacg gctccgtcgg
     8701 caggtgccag gccagcacct gcggcctgaa gcccttcgac acgtcgttgt ccttgtagat
     8761 ctccatgtgg atgaaggcgg cctgcttgcc gtacttcgcc ttcagctgct cggtgatgtc
     8821 ggtgactgga ccgcacaccc ggctctggca gagcgccggt gtagcgaaca gcagcacgac
     8881 cggctgcttg ccgagaacgt ccttcaggtt cacgtcgtgc atgtcgtcgt gcggctggcg
     8941 ggtgtcgacc ttctcgatcg agcctgccga ggcgaccgtg ggcgtgtcga ccgggatcgc
     9001 cttctgcccg acgtccggca cggggctgcg cgccagggcg cgcgtgggag cggcggggtc
     9061 ggccgccacg aggcggtcgt ccagccttgc cacgccgagc aggttgtagt tccccgcctt
     9121 cggcagcctg atgttggcca catagaccga ctgcgccgcg tcagggtcgt tcgccaccgt
     9181 ctggctgcgg tacggagccg ccacggcaag cgactcgtca tgcgccggga acggtccgag
     9241 cacgcggccg ccgctcgccg gctgcacgta gaccgccacc gcggcgtcgg tgatctgctt
     9301 gcgggcccgg tcgaagagcg cgaatgcgaa gcggttgcgg ccgggctcga gcacggcctg
     9361 cgcgggcgcc aacaccggcc ccgaagccag ctcctgtcgc atctcgacca gggagccccg
     9421 gtcgggcttc gggaagtcgg ccggcttggc cacggtcggc tggctgctgg tgtcgccgga
     9481 cgaccctcca ccgcctccgc acccggcggt cgtggccgcg gcgaacacga cgagcgcggc
     9541 gcacgcgagt ttcgtcctca ttcggcccgg aagcctagag agcagcggat gaaatgcggg
     9601 ttaagcccag cccggttagg gtggcgcggt tcacgcgact gagaggagtt ggttcatgga
     9661 agggcgtgct gccctcatca ccggcggatc gagcggcatc gggctggcga tcgcccgcgc
     9721 gctcgggcag gagggatacg ggctgaccgt gtccgcgcgc cggccggaaa agctcgagga
     9781 ggccgtaaag ggcctccagg acgagggcct cgacgcccag gcggtccagg ccaacatgac
     9841 cgacgaggac cagatcaaga acgtggtcga gcgccaccgc gacaagttcg ggcgcctcga
     9901 cgtgctgatc aacaacgccg gcgtcggcat cggcgggccg gtcgaggagg cggagaccaa
     9961 gaaggtcgac atgcagctcg acgtgaacct gcgcgccgtc tacctcacgg ctcgcgagtg
    10021 catcccgatg ctgaaggccg cgggcggcga gcatggcaag gcgctcatcg tcaacaccgc
    10081 ctcgatcgcc gggaagcacg gacagggctg gctcgccatg tactcggcga cgaaggcggg
    10141 tgtggtcggc ttcagcgagg cgctccagca ggagctcgac cagtcgggcg tgcaggtgac
    10201 ggccttctgc cccggctttg tggacacgcc gatgaccgac tggacgaagg agcaggtgcc
    10261 cgccgaggag atgatccggc cggaggacat cgcggaggcg gtgctgttcc tgctgaagac
    10321 ctcgccggcg tgcctcgtgc cggagatcca gttcgtgcgg ccgggggacc ggccctaggc
    10381 gaccggctcg cgatcgcgct gcggccactc gagcgagagc gggcggtcgc gctccgcggc
    10441 cgcttcgcgc tcgaactccg cgcggcgggc ggccttgatc tcctccacca cccgcgcgag
    10501 gtcgccggtc ggcaccccga ggctgccctc gagcatggcc tcgagcgtgc gcagctcgcg
    10561 tcgagtcgca cggcgcgggc tgccgatcgc cccggtgtag cggtcccagg cgcgcgcgaa
    10621 gctcaccatc gcggtgcggc cgccgcgccg gtgggcggca agcatcgggc acacgccgcc
    10681 gtcgcggtcc gtgtaagcac cggcgatgat gccgccctcg cgcacgcctt cgagcatcgc
    10741 cgtgcgcgtc ttctcgggca ggcactcgac agcggtgcgc agatctcgcg ccgggcttcg
    10801 cctcttccta tctcgcttcc ccataggatt ccggcctctt ggccgggcga cccgcaatct
    10861 acgcgcacta acgcgccgct tcaagacgcc ccggcctctg aaaggagact tgcatgcgcc
    10921 ttggccttca ggtgggctac tggggactcg ggctgactcc ggagcagcag ctcgagatgg
    10981 tcaagtcggc cgaatccgcc ggcttcgact cggtgtggtc ggcggaggcc tacggttccg
    11041 acgcggctac cgtactcgcc tggatcgccg cccagaccga gaagatcaag atcggctcgg
    11101 cgatcttcca gatccccggc cgctcgcccg gtctcacggc gatgaccgcg gccacgctcg
    11161 acaacctctc ggacggccgc ttcctgcttg gcatcggctc gtccgggccg caggtagccg
    11221 agggctggca cggccagcgt ttcccaaagc agatccagcg cacacgcgag tacatccaga
    11281 tcgtgcgcaa ggcgctgtcg cgcgaccggc tcgagtatcg gggcgaggtc tatgagctgc
    11341 cgctgcccga cggccctgga aaggccctga agctcacgat tgcgccggtg caggagcaca
    11401 tcccgatcta cctcgccgcg atcgggccga agaacacgca gctcgccggc gaggtcgccg
    11461 acggctggct gcccaccttc ttctcgcccg agcacgtggg cgagttccga ggcctgctcg
    11521 aggagggcgc gcagcgctcc ggtcgcgacc tcaacggctt cgacatcgcg ccgaacgtga
    11581 acctctgcat cgacgacgac gtggagaagg cacgcgacgc aatgcgcccg ctgctcgcgc
    11641 tgtacgtggg cggcatgggc tcgcgcgagc agaacttcta caacaagctg atgcagcgct
    11701 acggcttcga ggcggcggcc aaggaggtcc aggacctcta cctcgacggc aagaaggacg
    11761 aggccgcggc ggcgattccc gccgagctga tcgacaagac ctcgctcgtc ggctcggccg
    11821 accaggtgaa ggagcggctc gagctctacc gcgatgcgga cgtgggcacg ctgatcgtga
    11881 cgccgcttgc gtggtcgctc gaagaccgca accgcatgct caagacgctc gccgagctgg
    11941 tctgaccgcg tccgccgcaa aaccacgcct gagcctcctg ctcgccgcgt tcggcgatcc
    12001 gggccacgcg ttccccgcta tcgcgctcgg caaggagctc gccgcgcggg gacacgacgt
    12061 gtgcctgcag acgtggcgga agtggagcga acacgtggag cgggagggaa tgcgcttcga
    12121 gcccgccccg gagtacgagg tcttcccgcg cgagggcagt gcgctgaagc cctaccaggc
    12181 ggcggtgcgc gccgccgagg aaacggtccc gctgatacgc gagctcgatc cgcaggcggt
    12241 ggtggccgac atcctcacca ccgcggccgc gctcgcggcg ggaatggagt cgcgcccgtg
    12301 ggcgacgctc gtgccacacg tgctgccggc cggcgagccg ggcttcccgc tctactccgt
    12361 gggcgcgcgc tacccacgca cagcgctcgg tcgcgcgctc tggcgactgc tcgacccgat
    12421 cgaggacatc ggcgccaggc agggaaggcg tgagctgaac gaggcgcggc gccgcgtggg
    12481 cctgccgccg ttcgagcacc tgcacggcgg cacgtcgcgc cagctggcgc tgatcgccac
    12541 gttcccgcag ctcgagtacc cgcgacggcg ctgggagccg tggatgcgcg tgaccggacc
    12601 gctcatgtgg gagcagccgt acggcgacac cgacctgcct cccggcgacg acccgctcgt
    12661 gctcgtcgcg ccgagcacct cgcaggaccc gaagcagcgc atgctgcgag cggcgctcga
    12721 gggcctcgcg ggcgagccgg tgcgcgtgct cggcaccacc aaccgcaggg agccggcgga
    12781 gcagctcgaa atcccgccca acgcgcgcct ggtcgactgg gtctcgtatt cgcggacgat
    12841 gccccactgc gacgcggtcg tctgccacgg cggacacggc accgtcgtgc gcgcgctcgc
    12901 ctacggcgtg ccggtcgtcg cgtgcccggc cgcgggcgac atggccgaga acgccgctcg
    12961 catcgcgtgg tcgggcgtcg gcgtcgcgct gccgcggcgc cttgcgagcg ctcggggcgt
    13021 tcgcgccgct gtgcgccggc tcctcggcga cgaaagcttc gcgcgcaggg cagccgaact
    13081 cgcgggctgg gcgcgctcgc acgacggggc ggccatcgcc gcggacgagg tcgagcgctt
    13141 cgctagcgga cgcccgccga gcggcggggc ttagacgcga ccagcgcgag gtcggccatc
    13201 gtgaggagct cgttcgcgga catgccgtcc tcgagctgtg cgaccccgca gccgatcgac
    13261 agcgactcgc cggtcggctc gcggcacgtc acaaccatgc cctcgcgcag ccggtcagcc
    13321 gcggcgtgcg cgctcaccgc gtccgtctca ggcagcagga ccacgaactc gtcgccgccc
    13381 cagcggaagc ccgagtccat ctcgcgaagc gacgagcgga cggcgtccgc cacccgacgc
    13441 aggcagcggt cgccctccag atgcccgtgg cggtcgttga tgcccttgaa gccgttcacg
    13501 tcgatcacga gcacgctcag cgtgctcccg gtgcgctcgc aacgcgcaac ctccgccgcg
    13561 agcgactctt cgagcgcgcg gcggttgccc agaccggtga gcgcgtccac ccgcgcgagc
    13621 cggcgcgcgt cctcgccctc ctgccgcaag ccggcgcgct gcgagcgcac gcttgtggcg
    13681 tagatgctcg ccacgatcgc gatcgcggtc cagatgagga gctgcgaggc catttcgccc
    13741 gctgcgcggg tgtcccagcc gtcgtacgcc aggaacgcgc ccgacgccgc ggccaggaag
    13801 agcatgaacg cgccgagccg ccgcggagga ttgagcgagc cggtcaggag cgcccagggc
    13861 aggtacagct cgtggtagga gtcatggcgc gagccggcga gccactggag cagcgccacc
    13921 agcgcgacgc cgaggaagct cagcttgaac agagtctcgt gcgacacctg cttgccgcgc
    13981 accagcgcgc gaccacccgc gatcgacccg gcgacgatgg ccgcggccac cagccacccg
    14041 ggccagccga ggcgggcggt aggcggagca agcggcgccg ccaccatcgt aagcgcgccc
    14101 atcacaagcc agagaatccc gccgatcctg ttcgcgatca cgatgtccga gcccacgtaa
    14161 gggtcggcct ggcgggcgaa gagctgccgc agggagagca ggctcatgct gcgaccgcct
    14221 catcgcgctc gcgctcaagc gcgcggagga acgcctccac cacctgcggg tcgaactggc
    14281 tgccggcgca gcgccggagc tccgcgacgg cggcctcgag ccccatgccc ggcttgtaca
    14341 cgcggtcagc gcgcatggcc tcgtaggagt cggcgaccgc gatgatgcgc gcctcgagcg
    14401 ggatctcgtc cgtgcccaag ccgagcgggt agccgccgcc gtcggggcgc tcgtggtgcg
    14461 ccagcaccca ggcgctgatg tcctcgaggt tcgcgccgtc gaggatgcgc gcgccaagct
    14521 cggggtgctt gcgcatctcc gcccactcgt ggtcgtcgag cttgcccggc ttctgcagga
    14581 tcgagtcgga gatcccgatc ttgccgatgt cgtgcagcag cccggcgagg tgcacgcgct
    14641 cgactgtctc ctccggaagg ccgagctcgc gcgcgatctg ctgggcgtag cggccgacgg
    14701 tctgcgagtg cgtcgacgtc ccgctgtcgc gtatgtcgag cgtctccgcg agcacgagca
    14761 cggccgcgag gtgctcctcg ctctgagcct gctggcgtac gctcgcggcg cggatgttcg
    14821 cagacacctc cgcgttgaag atcacagtgc ggtcgcggcc gagctgcttc gcggcgctga
    14881 ccgcccgacc ggcgttcagc atcagctcct ccgaagagcc gccgtgccgc gggaagctgg
    14941 ccacgccgaa gctcgcggtg aggcccgagc tccgcgcgaa cgagtcgcgc accgcgcggc
    15001 gaacgcgatc ggccagcacg tacgcgccgt gctcgtccgt gtccggcacg accagcgcga
    15061 actcctcgcc gccgagccgg gcgagcgtgt cgatgcggcg cttgcgcgtg gcgagcacct
    15121 tggccacccg gcggatggcg tcttcgcccc tgcgatgccc gtgctcctcg ttgaagtcct
    15181 tgaagcggtc gatgtcgcag accacgatcg acatccggga accgctgcgc tgcgcgcgct
    15241 cgagctcgcg ctcgatcagg tcgtcgaagc cggcggcgtt gagggagccg gtaagcgcgt
    15301 cggtctggtg agccttgctg atgtgggcga cgaggcgctc gacgcgagaa cgcaggaagt
    15361 cgacgagccc tcctgccacg cacaccacca cgagcgtgag cagcaggcgg tcgagtggga
    15421 agccgtcgcc ggcgtcgagc gcgagcagtg cggtgtacga caccgcgatc agcacgatct
    15481 gcccgatcgc gccgcgcctc gagaggaaga agaagccgtt gagccctgac cagacataga
    15541 gcagcgacag gtcgctcgtg gcggggtcgt cgagccagac cacgagaccg atcagcacca
    15601 ccccggcagc catgcccacg ttgaggaccc aagtcggaag gcgcttggcg aagaggacga
    15661 gggttgcgcc gagcacggac gcgagcacgg caagcgcgag gagaagcgag cgcgacacct
    15721 gaagcgtgcc cggcaaggcg agcgccacgc cgagggctgc gcccccgagg tagctgaagc
    15781 cgagcgcccg aatgatcgcc accgagctcg gcaagagcgc aggcggaagg gccgcgctgt
    15841 ccagcgagcg ttccggtagc ccgacgggtg acggcaatgc gtccatcccg tcatttactc
    15901 ggccagtttg ggccggacta aagcctcagg aagcgacttg cgcgcacggc acgaggaaaa
    15961 gctccccggg taggactcga acctacaacc tagcggttaa cagccgctcg ctctaccatt
    16021 gagctaccgg ggaatgcggc gttcgcaagc gtagcgaacg cttccgcgag caccttgacg
    16081 tggcgctcgc atcaagccgt ttatcggctc aggtttcttc ggctgcagtg ccctcgaggc
    16141 ggcccgcgtc ggcgttcttg agcttcatga gcgtcttcga ctcgatctgc cgcacgcgct
    16201 cgcgcgtgac ccccacctcg ctgccgatct gctcgagcgt catcggtccc tcgcctacga
    16261 gcccgtagcg cagctcgagc acgcggcgct cgcgccacgg cagcgtctcc agggccgcct
    16321 ggaggtcggc ctcgcgcagg cggttggcga cggcctcgag cgggcggtct gtggcctggt
    16381 cggcgatgaa gtcggcgagc tgggcgtcgc catcctcgga gccgaccggt gcctcgagcg
    16441 acaccggctc ctgcccgaac ttgaggatct cctcaacgcg ctgcggcgtc atctcgagct
    16501 ccttgccgat ctcctccggc gtgggctcac gaccgttctt ctggatcagc gcgcggcgta
    16561 cgcgggcgac gcgattcatc cgctccacca tgtgaaccgg aatgcggatc gtgcgcgact
    16621 ggtccgcgag cgcgcgggtg atcgcctggc ggatccacca cgtggcgtag gtggagaact
    16681 tgaagccgcg gcgccagtcg aacttctcga cggcgcggat gaggccgatg ttgccctcct
    16741 ggatcagatc cagcagggtg agcccgcgcc cggtgtagcg cttggcgatc gacacgacga
    16801 ggcgcaggtt cgcctccacg agcgcgttct tcgccgtcat gtcgttgcgc tcggcgcggc
    16861 gggcgaggcg cacctcgtcc tcacgcgtca gaagcggtac ccggccgatg cgcttcaggt
    16921 agaggcggac gctgtcctcg tggccgccct ccggcggtgc cgcgggcagg ccggaggtga
    16981 cgacgccacc ctcctcgtag aggtcggcga cctcttccgg ttcctcgctc tgactctctt
    17041 cctgctcgta gtcgcgctcg ggatcatcga gcgcggcgct gaagctgctg tcctttgcgg
    17101 tcactacgcc tctcccatga cgtgtgcgat gcgctccctg acgtcctcgc gcgcggacca
    17161 gagccggcgc tggcggtcga agtcgcctgt ttcccctgcc tgacgcagat cgcgctcgat
    17221 ccgccgcagc tccagctgca ggaatccgag gcggagcgca ggctccgaag agggctcgtg
    17281 ctcggcgagc atagctacgt tcgtcacgat tgccgacact tccggatcgt ctggtgaaag
    17341 acctgcaagc gggtcgtcga agtgatcggc gaggtggtcg cgcacgcggc gcagcgcctc
    17401 ggatcccagg tgctcatcgg tgagctgcga caggtaggag cgccctgcct ctccctgggc
    17461 gaggcacatg gccaggaagg cgcgctcggc acgagtcgcc tggtcgatcg agaagcgcgg
    17521 ccgcgctccg ttgtcgcgcc gctcgcgcgc ctgcgtggtc ggctcgctca gcgttgtctc
    17581 gaggtagtgc acgggcacat cgatccggtc cgccgcgtag cggacgacgg cgtcacgggt
    17641 ggcggtcttc tcggggatcg ctgcgatcaa ggggcgtacc tgttccagag cgcggtctcg
    17701 tccgcgcgga gtactgagat ctgaatcggc aagcacacgc cgaacctgaa actcgggaac
    17761 cgtcgaagcc gagtccagaa ggcccttgaa cgcctctgca ccacgctccg tgatgaggtc
    17821 ggccgggtcc tcgccctcgg gcatgtcgac cacgcgaagc tcgatgccgg cctcgtccgc
    17881 cgcgcgagcg gcgcgcagca tggccgcctg gcccgagcga tccgcgtcca gagcgaggaa
    17941 aaccgtgccc gcagcgcgtg agagctcact cagctgctcc tgcgtcaggg cagtccccat
    18001 aatcgccacg gtctctgtca ctcccgcctg gtgaagagcc aagacgtcag tgtaaccctc
    18061 cacgaccact atgcggccag attttgcggc cgaagctcgt gcaaggtcaa taccgaagag
    18121 ctgcttccct ttgtgataaa ggtcactttc ggccgtgttg atgtacttgg cctgctggcc
    18181 ctggccgagc gcgcgagcgc cgaaaccgag cgtccgccct cgcgagtccg ccagcgggaa
    18241 catgatccgt tcgcggaagc ggtcgtacat gcgcccgccg cgccctcggc ggccgaggtc
    18301 cgctgcttgg acctcctgct cgctgaagcc gtccttcagc gcgccgctca cgaccttgtc
    18361 ccaggcgctc ggcgagtagc ccacgcggaa ggtcctgaga acctcctcgt tcaggccgcg
    18421 gccgagcagg tacttgcgcg cgccggcggc ctcgcccgaa tcccagaggt agcgcgcgta
    18481 gtagtccgcg gtgcggtcca cgagctggag cagccgctcg cgccgtcggc ggcgttcttc
    18541 cgcctcggga tcctccttct cgagctgcag ctcgacgttg tagcggtcgg agaggaactc
    18601 gagggcgccg cggaagtcga gaccctgggt ggcctccacg aacctgatcg catcgcccga
    18661 ttcgccgcag ccgaaacagt ggtagagctt gtgctcggcg ttcacgctga aactgggcgt
    18721 gcgctcatcg tggaaggggc agagcccggt ccagcgcgtg cccacgcggc gaaggtcggt
    18781 gcgcgacccg accaggtcca ccatgtccac ggcctccttg agccgctcga ccgaatcccg
    18841 cgtgtagagg gacatgaccc ggcgagccta gtcggctaga agccctgcgg cagagaaagc
    18901 tcggcgaacc ggcggagcgc gaagcggtcg gtcatgccgg cgaggtagtc ggtgacgcgt
    18961 tcggcctcgg cggcgtccgg cgtggccgcc ggcggcgggt cggccgcgag gctgtcgaag
    19021 agggcggtga gcatgcgctc gatccgcggc tgctcacgct gcgccaccgg gccgaggtag
    19081 acggtttcga acatgaagct gcgcagccgc gccatcgcct cccccacctg ctcgctctga
    19141 actatgtctc cctcgcggtc cgagcactcg accaggtcgc gcaccagggt gtcgatccgc
    19201 ttcgagcccg tgtcgcccag cgcgtcgatc tcgcgcgcgg ggaggtcctc gtaggcgagg
    19261 atgccggcgc gcacggcgtc gtcgatgtcg tggttgatgt aggcgatgcg gtccacgagc
    19321 cgtacgatcc gcccttcgag cgtgcccggc aactcggggc ccgtgtgcct gaggatgccg
    19381 tcccgcacct gctcggtgag gttcaggccg cggccgtcgc gctcgagccg ctccaccatc
    19441 cgcagcgagt ggtggttgtg gcggaagcgg acgccgtagc ggcgctgcag gcagccgtcg
    19501 agcacctgct ccccgatatg gccgaagggc gggtgcccaa ggtcgtgccc gagcccgatc
    19561 gcctcagtga ggtcctcgtt cagcgcgagg gcacgcgcga cggtgcgggc gatcatgcag
    19621 gtctcgagcg tgtgcgtgag gcgcgtgcgg tagtggtcgc cctcgggcga gatgaacacc
    19681 tgcgtcttgt gcttgaggcg gcggaaggcc ttgctgtgga cgatccggtc ccggtcgcgc
    19741 tggaacgggg tgcgaaccgg cgagtcctcc tctgggagcg cacgcctcgc ggggtaggag
    19801 cgctgcgcga gcggcgacag gaacgcgtcc tcgaaggcgc tctggcgctc ggcgagcggg
    19861 atctgctgtg gctggactgc ctgcgaggcc atgtaccgat tgtcgcagcg agcgcggcgg
    19921 cgtcttgtgc gggttccgca caatgcgtga caaatcggca cgcggcgggc ggtgtgccgg
    19981 taccgcggtc gattttgggt gcatagggcc ccaaaaagca ccacgctaac ccggaaccct
    20041 cctggaactt ggcgccagat cgcttcgcgc ttcgacaccg caactcgcac cgttccgcgc
    20101 agcaactccg aaatcggaag gtgccggatg ccggcgcgca cgtgcgggat cgcactaaaa
    20161 gcgccgtagg gcgggccgat cacccggggg tccccggccg ccgctggtcg ggcggaccaa
    20221 cgaaggaccc ttggacccgc ttcgccgcca catcgcactg cgcgcggcgc tgctcgcgtt
    20281 cgcggcagcg ctgctcgtgc ttgccgtgcc cgcgtcgccc gcggccgcca acaagcgcgc
    20341 cagcgcgtcc gccgccgcca ggatcgtctc gctcgcgcgc aaggagctcg agcgtggcgt
    20401 gcgcgaaatc cccgacggct ccaacaaggc gcccgcgatc tggcgctacg acgaggccac
    20461 caccccgcac tggtacggcg cgccctggtg cgcctacttc gtctcctacc atcgcgaagc
    20521 gcgccggggt gccgatcggc cccggcggca agggactcgg ctatgtgccc tacatccgct
    20581 cgtgggcgaa gcagaccggc cgttggaccc gcaagccgta ccccggcgcg ctcatcacct
    20641 tcccgcagca cgtcggcatc gtcgagagcg tctactcgaa cggcacgctg acaacgatcg
    20701 agggcaactc cggtaacgcc gttcgccggg tctggcgccg ctggcgcgac gcgatgggct
    20761 acgtgaagat cgcgagcggc tcggtgccgc gcaagcccgc gcccgcggcc aggagaagcc
    20821 caagccgacc ggccccgccc agccgcttgt cgcgcgcatc accgcgtacc cgtcgaagaa
    20881 cgtgggcgtc gggcagacag tcgacttcac gagcaacgac tcctcgggcg acatcgcgaa
    20941 ggcgcagtgg gacctcgacg gcaacggctc ctacgagcag accggcgaca acgtgaagcg
    21001 ccgctacgac aaggccggta gctatctcgt gaagctgaag ctcaccgatc ggaagcacca
    21061 caccgccacc gcgacgatca cgatcacggt gacgagcaat cgcgcgccgg ttgctgtgct
    21121 cgacatgccc acgcagatcg aggtcggcaa gagcttcaag gcaagcgcca cacgctcgag
    21181 cgaccccgac ggcaagatcg tcgcctggta ctgggacctg aacggtgacg gtgactacgg
    21241 cgccgacggc tcgagccgct acttcaccta cgacacgccc ggcgactact acgcgggcct
    21301 gcgcgtgaag gacaacgacg gcaacgtgag cgaggtgcac gtgccggtcc acgtctacga
    21361 ccacccggac ccggtctcga agctcagctg cgacgccagc tcaatcccgt cgggcggcac
    21421 gctccactgc cacgccgacg actccgcctc ccccacgaag atcgtgaagc acgagtggga
    21481 catgaacgac gacggcagct acggcaccgg cggcaacgac agcagctaca cgtactcgac
    21541 ctccggctgg tacacggtgc acctgcgcgt gaccgatgtg aacggccgcc gctcggagct
    21601 gagcacggac atccaggtga ccaaccgcgc gcccagcgcg cgcatcacgc ttccggccaa
    21661 ggtcgtggtc aaccagccgg cgagcttcgg aggcggcggc tcgagcgatc ccgacggcac
    21721 gctcttccag tgggagtggg actgggacgg cgacggtttc tacgacgcgc tcggctcgtc
    21781 cgcctcgacg accttcacga gctacggcca gcgccggatc ggactacggg tcaccgacaa
    21841 cttgggcgca accgcggttg ctaccacgac cgtgaacgtg ctcgcgcctc cggtgtcggt
    21901 cccgcggatc ctgaccgcaa cgccgctcgc cggaacggcc acgagcttcg atccggcgct
    21961 ctcgtacgac cccgacgggc gcccgctcac cgtgttccag tgggacttcg acggcaacgg
    22021 cacggtggac cggacgacca cgaccgcgac gccggtgacc aagacctact cgggccgggg
    22081 cagctacacg gcgaagctga cggtgaccga cgcggacggc ctaaccggta ccgcgacggt
    22141 caccgtgtcg gtcaattagc cggcgagcag tgagaacgtg tccgtggacc tgtcgttcct
    22201 ggtcaccgat ttgaaggttc cgcgggctcc gttgtaggcg ccggtgccac ccgtgatcgc
    22261 ggcagtcgtc gtgttctcgg agaacttcgc ccgggcctcc agagtcatag tgccgccggt
    22321 caagctcgcg acggcgttac agatgaactg ggactgttcg aagctccggc ttcccttgac
    22381 cacggtgcag gtgccctcga gcgtcccggc gaccttgccc gcttgatcgg ccaggcgcgt
    22441 gctgaacatg atcaggtcgc ccggcgacga gctcaccggg aagccgtgcc gcttacgcga
    22501 cagtggcgca ttgtcgacga agttgaacgt cgagccccgg ttcagctcct tgaaggtgag
    22561 cgtccgggcc ccgggcgcct gggcacttcc gccggatgcg acgagggccg cggtggcgaa
    22621 cccggcgatc gcgagcaccg ccaccggcat gagacgtgag cgcatgatgc tcctcctgcg
    22681 tcagccacgt ggacgcacga atcacacgct acgtccggtc cagcagcaga tgcgcgccaa
    22741 gacggcgcta tctgtacgag ctgaagcccg cggcgcggat gtacatctcg tagagcagga
    22801 agccctgcgc gacgcggctc gcgcacacgc ggtcgaacaa cgcgtttgcg cgcgcggtcg
    22861 agagcgtcgt gccgacgccg cccgaggtcg agggcccgcc cacgactccc gcgagcgcac
    22921 gcacgatcgc gccgcgctct gacggtgtcg cgccgatcca ctcctggcag cgcgcttgct
    22981 ggagcggcag tccgccgatc agtgcggtcg gagtgaacgc ggcgcggtgc cggaccggcg
    23041 ctgcgacgtc gcgtagcgcc agcgcgagca ccccgctcag cgcgagcgag gcggcgagcg
    23101 cgagcgccag cacgcgcccg ctgagccgca cgcgccgcgg cgcgacggct aacgcttggc
    23161 gaacggactg catatgaccg tccctcccag tccgccgatg aggtcgcaga agggcggcgg
    23221 cggctcagtc tcgcccacca tctggtttgc gatttgttgg ttgcgcagcg tctgctgctt
    23281 cgccgcagcc gtgcccggct tggccggcat ctggaaccac tcgcacgtgt tcgggtcgta
    23341 gttcccggtc gcaagggcgc ccgcgcagct ggcgagcatc gcctccttcg cggcgtcgcc
    23401 ggctcccacg ttcgtgggcg cggagcagtc ctgcggcagg aacttcggaa tcgaagtgcc
    23461 cgggacgggc ggtggcggcg ggtcggatgt gaccgaagcc tccgagccgt cagcgccctg
    23521 gttgttgtac cagcagttgt tgctgttcgt cgggaattcg tcccaccaga agtccacgcc
    23581 gttcggcgcc ttgccgccgc tcggcgtccg gctcatcacg ttgtcgaagt agcggttgcg
    23641 gttcgaaacg gatgcgatcc cctgcggggt gcagggcggc gctccgttct ccggcgaggg
    23701 cgcgcagctg atcacgtcgg gcaccgcgat gagcattgtg ccgcggcgcc agttgtccca
    23761 gatgcggttg cccttcacct ggtcgtcgtt gccgccggca atgaggatgc cgacgccgat
    23821 cggcaccggc acgcgcgact ccacgtccga gtccttcgcg tagacgttga agttgttcga
    23881 ccagatccgg ttgcgctcga acacggccga gtcttgcgga tagccggggt ggccgcccgc
    23941 aaagaacgag tcggtcgcga tgccggtcgt gttcccatag aagttgttct ccaccacgcg
    24001 ggtcgcgttc cccatcgtcc ccgagtaacc gaggttgttg tggtgcaggt cacagtgcgt
    24061 gatcgtctgg ttcaaacggc gcgtgggata gaagctgtcg tcccgctgcg cgccggtgtc
    24121 gggcgcgccg cccggataca ccgccgagtc gccgttaccg acggcgtcgc acgtgtcggt
    24181 gagcccgtgg tctgacgcga acatcagcgc gccgtactca cctccgtaat agaagttcat
    24241 gcgctgcagc cggtagccgt ccgtctcgag cacgtacacg tcgtgctctg ccgcatggcg
    24301 gacggtcatg ttcttcagca cgaagccgtc ggcgcgatcg gcctttatgc ccacgtcctt
    24361 cttcgcgtcg atcggtccgt ggttgcccga ggcaacgcgg ccggcgtcga ccaccacgct
    24421 ctggggtgtc gggcccgagc cctccatctg gacgttgcag cggacgcact tgccgaggtt
    24481 cgggatgccg tgcgggtcag gccgacccgt cgggctcgac tgcggttcga gccccgtccc
    24541 gagcgcgcgg ccgatcaccg cgacgagcgc ctgggcgttg ggacagtggt actggtactc
    24601 gtacgagacc gctcccgatc cgtgatccga agttgtcttg tactggtcgc actccggcgg
    24661 gaacggcgac accgcccgcg aatcgggctc ggtgtaggtg ccgggcatca cgaccacgcg
    24721 gtcgttgttg cccgatgcgg tcacggccgg ctggatgtcg tggtagcggc acttgtcgaa
    24781 cagcgccgtg ttgacgtcga tcagccgctg cgccgacgcg gcgctcagct ttcgcttccg
    24841 ctcggtcggg cggaagcggt agcccttgtc gcgcgccgtg gcgacctctt ccttcgcgac
    24901 gctgagcgag tcgctcttgc acaccacgcg cgtgtcgccg cgagccttcg tatcgagagc
    24961 cgatgcgatc gagcgtgcgg ctggaacctt gcctccggca gcaggccacg tggatgtgtc
    25021 gggcgcggga tccggccagt acgccgtccg ctccacgtgc gcgagcgcgc tcgacactcc
    25081 tgtgagcgcc cccaccgcga tggccaacgc cacaaaggcc ttccgcccca tctgaacccc
    25141 cgccggttct cacggcgccc cctgcagacg ccgaactggc gcgaaaacct acctggtcgt
    25201 caggaactgt gcgccactgc ggcctgtgct gcggccacgc gcgcgatcgg cacacggaac
    25261 ggcgagcagc tcacgtagtc gagccccgcc atgtggaaga agtcgatcga gtcagggtcg
    25321 ccgccgtgct cgccgcagat gcccagcttg agctccgcct tggcctcgcg gccagtccac
    25381 gccgcgaggc gtacgagcca ccccaccccg ggcttgtcga tcgtctcgaa cgggctgcgg
    25441 tccacgatct tctcgcggat gtactcggca aggaacttgc cctccacgtc gtcgcgcgag
    25501 aaaccgagcg cggtttgcgt gaggtcgttc gtgccgaagg agaagaagtc ggcctggccg
    25561 gcgatcttgt ccgcggcgaa gcacgcgcgc ggcagctcga tcatcgtgcc aatcgagcgc
    25621 tcgagatcgt cgccctcctc ctcgaagacg tgctccacga gctcgcgcat gatcctcagc
    25681 tcggtctcgt aggcgactag cgggatcatg acctcgaggt gcggcgcctc gccgcagcgc
    25741 tccttgactg ctttggcggc gcgaatgacc gcgcgcacct gcatctcgta gatctccgga
    25801 tggacgatgc cgaggcgaac gccgcgggtg ccgagcattg gattcgtctc ctgcagctgg
    25861 tggacgcgct ccagggtgcg ttcgagctcc tcgaggtcgt cggtctgctc gatgcgcgca
    25921 cgctcgacgt cctgggcgat ctcctcgagc ccgggcagaa actcgtgcag cggcgggtcg
    25981 agcaggcgga tcgtgacggg cagtccgcgc atctgttcga acagcccctc gaagtcctcc
    26041 tgctggaacg gcagcaggtc gtcgagggcg gcgcggcggt cctgctcctc ctcggccatg
    26101 atcatcgcgc gcatcttcgg ctggcggtcg gccgccatga acatgtgctc ggtgcggcag
    26161 agcccgatgc cctccgcgcc gaactcgcgc gccttctgcg cgtcctcggg cgtgtcggcg
    26221 ttcgtgcgca cgccgagcct gcggatctcg tcggaccact tgagcacctt ctcgaagttc
    26281 tcgtcgacct ccgggtccac gagctccacg tcgtcggtcg tgacgacacc gctggtgccg
    26341 tcgatcgcga tgaggtcgcc ctggtgcagc accgtcccgt tgacgtgcac ctcgcgcgcg
    26401 ttcaggtcga tgtggagatc cgacgccccc gaaacgcagg gccgccccat gccgcgggcg
    26461 acgagcgccg cgtggctcgc cttgccgccc tcggacgtaa ggatgccgcg cgcggcgaag
    26521 aagcccgcga cgtcgtccgc ctcggtgaag ggccgcacga ggatcacgtc cttgcccgcg
    26581 tcgccctgct ccaccgcgtc gtcggccgtg aacacgatcg ctcccttggc cgcgccgggc
    26641 gaagcgttca cgccacgggc gagcacgtcg aactcggcgt tcgggtcgaa ggtggggtgc
    26701 agcagtgcgt cgagcttcgc ggcatcgatg gtggcgagcg cctcttcgcg tgagagcagc
    26761 tcctcgtcga ccgcgtcgac cgcgaagcgc acggccgcct gcgccggccg cttcgcactg
    26821 cgggtctgga gcatgtagag atgcccctcc tcgacggtga actccgtgtc ctgcatgtcc
    26881 ctgtagtgag actcgagcgt gcgcaggatc tccatcagct cggtgtgggt ctccggcatc
    26941 acctcgttca gctcggagat gtcgcgcggc gtgcgcacgc cggacaccac gtcctcgccc
    27001 tgcgcgttga gcaggaagtc gcccgacggc tcgggcgcgc ccgtgatctc gtcgcgcgag
    27061 aacgccaccc ccgtgcacga ggtgtcgccc ttgttgccga acaccatctg ctgcacgttg
    27121 accgccgtgc cccagtcgtc cgggaggcgg ttgatgcgcc ggtactcgac ggcgcgctcg
    27181 cccatccatg agtcgaacac cgcgcggatc gcctggcgca gttgctcctg ggggtcttgc
    27241 ggaaactctt cgctggtctg gtcgcgatac agctgcttga aggagtcgac cagctccttg
    27301 aggtcgtcca cggagagctc cgtgtcgtcc ttcacaccgg ccgtctcctt ctgggccttg
    27361 atggcatcct cgatcttctc gccgggaacc ccgcggcaga cgttgccgaa catctgcacg
    27421 aagcgccggt acgagtccca cgcgaagcgc tcgttgtcgg tgacctttgc gagcccctcg
    27481 acggactcgt cgttcaggcc gaggttgagg acggtgtcca tcattccggg catcgactcg
    27541 cgcgcgcccg agcgcaccga cacgagcagc ggatcgtccg ggtccccgag gcgcttaccg
    27601 gtttgctcct ccaaatgcgt gagggcctcg gcaacctgct gctcgaggcc ctcgggctcc
    27661 tcgcggcccg cgttcatgta ggcgacgcag gcctcggtcg tgatcgtgaa gcccgcgggt
    27721 actcgctcgg cgcccaggat gcgcgtcatc tcggcgacgt tcgcgccctt gccgccgagc
    27781 agctcgcgca tgtccttcga gccctcggag aactcgtaga cgtacttcgt ggcttgtgcg
    27841 gtgctggtca tgaggggcgc gggattgtat ggagtgaata cccgggattg ggccggatga
    27901 gtaaacggcg aagagcccgc ctggcaagga agcagggccg aagcttggcg acagccaagc
    27961 gagcgcccga tgacacagcc aggcgatgct ctgcagccga attactcatt cggccttcgc
    28021 cacatgagcc agacgggagg gcctttcggc agctcgagcc gctccgtcac ctcgaagccg
    28081 tggcggccgt agaaggcgag gttgcgctcc ttggccgtct cgaggtaggc cgggatccct
    28141 tcactgtcgg acatctcgag gacggggcgc agaagcgccg agccgatgcc ttgcccctgg
    28201 tggtcgggat cggtgccgag cacggccagg tagaagtgct tctccctggg gtggcgccgc
    28261 tccaccatcg tgagcccgcg cgtcacgagc ggcagccggc gcgctcgcac gagcaccatc
    28321 agcctgagcg tctcgccgat cgggacgagc cacttgcccg gcagggccca cagcgctgcg
    28381 ccctcgcggt cgtgcgtcgt gtaggagagc tcgtgcggga tgagcgtccg cagccgcagt
    28441 tcgaagaagc gcgccgccgt cctcaggcgc gtgggctcgc ggtcgaaggc ccagcaggtg
    28501 accgggtcgt cgaggaaggc gcgcgcgagc acgctcgcga gacgggtcac atccgactcg
    28561 ctgacgcgct ccaccgacgg ggcctcgagc acagacctac gctacccgag cgagccttac
    28621 gtgcgcgtgg tgctcgagcg tctcggtgat cgcgcgatcg gcctgcctga gcgcgctctc
    28681 cgacgcgtcc gggacgtcgg agagcgagcg tccgagcgcg tccacgagga agcggtgggc
    28741 gctctcgtcg agctggaacg agcccgcctc gcagcctgcg cagacgacgc cgcccgcgct
    28801 cgccgagaag gcgccaaggt gctcgcgctc gccacaggag gcgcacgcgc ccagctcggg
    28861 cacgaagccg gcggcgagca gcagcttcag ccggaacgcc agcgcttggg cgcgcgtggc
    28921 ggccgccggg tcagcgtcga gcagcgcgag ctcgtggcag agcaggttgt aggcaggcgc
    28981 gttcggctcc tcggagtcga acagccgcag caccgcctcg cacgcctggc ccgcgcgctt
    29041 catcggctcc gcctgctccc gcaggcgcgc gtgcgggtga atcgtggagg cgcccgtgat
    29101 cgtgcaaagc tcgccgctgc cctggtggca gacgagctcc acgcgcacga gcggctcgag
    29161 ccggcccccg aagcgcgact tcacacgcct gacgcccttt gccatcgctg cgatgcggcc
    29221 gcgctcccgg ctatagaggt gcaggatgcg atccgcctcg ccgtagcgga aggagcgcag
    29281 caccaccgcc tcggtcttga acaagccggc catctctaca ctgaatcacc agtggcggac
    29341 aacggacgcg tgacggttta cacaaccgaa ccgtgcggat tctgtcgcca ggccaaggcg
    29401 ttgctcgagg cgcgggggat cgtctacgaa gaagtgaacc tcgcgaagtc ccctgagggg
    29461 cgcgcggatc tggtgcagcg cacgggccag atgacgttcc cgcagatcct cgtcggcggg
    29521 cgcccgatcg gcgggttccg cgagctggtc gaggccgacc gcgacgggtc gctgaagacg
    29581 ctgctcgctg ccgcttagac gcttgcccgc cgcacgcgcg gcctgggct
//
LOCUS       DASVXS010000208        10741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1231414, whole genome shotgun sequence.
ACCESSION   DASVXS010000208 DASVXS010000000
VERSION     DASVXS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231414"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VF752_11620"
     CDS             complement(<1..1204)
                     /locus_tag="VF752_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8856237.1"
                     /translation="MHLRSLTLKGFKSFPDRTRLDFSPGVSVVVGPNGSGKSNITDAV
                     LWAMGEQSPLAVRGQTMQDVIFAGGHGIASRNSAEVEVVIDNSDGGIDSEFSEISIVR
                     RLERSGDGEYRLNGARCRLVDVLEVLSDTGLGKEMHSVVSQGRVESIIHSKPRDRRLL
                     IEEAAGLGKHRKRRRRAQLKLERTQDNLDRALDVEREARSRLRPLKRQAEAAELHERL
                     ERQSLEARLELARDRARGAIAALAEAEAVAAAAAGRRDEVEKSLAEVARRREAAEEAF
                     AEHSRAREALSARAFAARSAVDRIGLRLERAREIARAGAEGAVRRRREVEALVERDGD
                     PSEGGAAGVAGALEARESEHSARIAELEREVVAVEAERTAGLEAEIAKLASERDTAEE
                     RRGEVEAAV"
     gene            complement(1209..1886)
                     /locus_tag="VF752_11625"
     CDS             complement(1209..1886)
                     /locus_tag="VF752_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEX8856238.1"
                     /translation="MDTLSSLLDELPDDLARQAFTHSSWVDNRWESYERLAFLGDVVL
                     SLAISTDIYPRFPRYGAGRLTKLRAQAVSRHACAEVARDLGVPERLRAAAPEGTGKSA
                     EMLIASDRILASICEAIIGATYLAFGFERVAPAVVYAFAEQVEEALEHPVDYKSVLQE
                     RLARRAQVVAYRIDDEAGPPHDRSFVAVAEVSGREIGRGEGRTKKSAEQEAALRALDS
                     LAQEQEG"
     gene            complement(1890..2144)
                     /gene="acpP"
                     /locus_tag="VF752_11630"
     CDS             complement(1890..2144)
                     /gene="acpP"
                     /locus_tag="VF752_11630"
                     /inference="COORDINATES: protein motif:HMM:NF002148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8856239.1"
                     /translation="MTREEVLERIRAHLATELVRDPSSIGEDTRFKEDLEADSLDLVE
                     LVMELEDNYGVRIPDEEAAKILTVGQAADFVAAHAPQNAP"
     gene            complement(2137..3132)
                     /gene="plsX"
                     /locus_tag="VF752_11635"
     CDS             complement(2137..3132)
                     /gene="plsX"
                     /locus_tag="VF752_11635"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX8856240.1"
                     /translation="MIALDANGADGGPRTVAEGGRRSGVPVTLFGPAAELRDGAEVVD
                     APVAIGNDEEPVRAVRSKPDASIVQAAKAVAEGRCSALVSAGATGAALAAGVLHIRRL
                     QGVHRPGVAVFLPIPGAPTLMLDVGANLEVRPEHLVQFAFMGSAFMQTMHGVERPRVA
                     LLSVGEEPTKGTPDVVAAHERLAGASGIEFIGNVEGTDVPAGAADVVVTDGFTGNVAL
                     KLMEGTAKTVVGAIRDAVRSGAVSSLGGLLIRRRVSALRDALDPNATGGAILLGLRRP
                     VVIAHGGSSADGIANAVRLAERAVREDAVGRMAAALEAGGALRSAPVDSVEVAHD"
     gene            complement(3142..3336)
                     /gene="rpmF"
                     /locus_tag="VF752_11640"
     CDS             complement(3142..3336)
                     /gene="rpmF"
                     /locus_tag="VF752_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX8856241.1"
                     /translation="MAVPKQKQSHSRTNKRRSQHKITPPALGTCPQCHSPRLPHRVCS
                     VCGTYGGREIFTPSGSGADE"
     gene            complement(3406..3921)
                     /locus_tag="VF752_11645"
     CDS             complement(3406..3921)
                     /locus_tag="VF752_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX8856242.1"
                     /translation="MGQRTDIFDLGRLNLHSGEGRRIETAVNVDPIDLGGQEYGVEDR
                     VAHVVLDVSHTTTGYSLRLRTNVTLVGACVRCLEPAERSVEVDVREVDQPGGDDELRS
                     PYVEGDDLDLRSWTRDAVIFAMPAQVLCTDECLGLCPVCGENLNSAGADHRHEEARDP
                     RWAKLGELRFE"
     gene            complement(3969..4700)
                     /locus_tag="VF752_11650"
     CDS             complement(3969..4700)
                     /locus_tag="VF752_11650"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8856243.1"
                     /translation="MAAVLACGAGAALSHHSAAVLWRIRKQQLRGIEISVPAARRSHR
                     AGINIHRRRAFSDDDVTECHGIPVTTPICTLIDLAATTLSDNELEAAVNEADKLDLAD
                     PEALRAALDHHGKRPGVTRVRRLLDRRTFALTDSELERRFLPIARRAGLPMPKTQQLV
                     NGFKVDFFWPELGLVVETDGLRYHRTPAQQSRDALRDQKHFAAELTPLRFTHAQVRYE
                     PDHVEATLRAAARRLRLASSEDAAS"
     gene            complement(4955..5416)
                     /locus_tag="VF752_11655"
     CDS             complement(4955..5416)
                     /locus_tag="VF752_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856244.1"
                     /translation="MDVLVLIDKLDDQIHNAKPVPLTDQVRVDKEEIYDILDQMRATI
                     PEEIKQARWIVKERQEMLAEAKREAERIIKEARDEQARLIAQEEIVRQAERQAEDIIE
                     EARGREREIRLGAEDYADDILNTLEVNLQKFTAAVQRGRERLAGREEAEVR"
     gene            complement(5433..5936)
                     /gene="coaD"
                     /locus_tag="VF752_11660"
     CDS             complement(5433..5936)
                     /gene="coaD"
                     /locus_tag="VF752_11660"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX8856245.1"
                     /translation="MFSDDGKGTAVCPGSYDPVTFGHLDIIRRTAEVFDTVVVGVVNQ
                     PVRKQSTVFTADERVSFIEAEVGKLGNVQVKSFDTLLVDFARQHGAKAIVKGLRAISD
                     FEYEFEMNQLNRKMAPDIESIYMMSSPQYSFLSSSGVKELAMFGGDLTDLVPPQVAQR
                     LEEALKR"
     gene            complement(5926..6462)
                     /gene="rsmD"
                     /locus_tag="VF752_11665"
     CDS             complement(5926..6462)
                     /gene="rsmD"
                     /locus_tag="VF752_11665"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX8856246.1"
                     /translation="MRIVAGSLGGRRLHAPPGRGTRPTADRVREALFSILGDVSGLAV
                     LDLFAGSGALGIEALSRGATSATFVDSGTRAIAAVRRNLAELGLDAPVIRRDALAFLA
                     APARAPGAPYDLVFLDPPYSSADRLAGPLSERLPAVASGRGLIVSESDKRNPLELTLP
                     LEDERTYGDTRIALHRVQ"
     gene            complement(6459..8519)
                     /gene="recG"
                     /locus_tag="VF752_11670"
     CDS             complement(6459..8519)
                     /gene="recG"
                     /locus_tag="VF752_11670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX8856247.1"
                     /translation="MRSWPRPLRLAEEPPLPKRAREPAAAIGITTVGELLEHIPRAHT
                     DLRERRTIASIGIEEEATVEAQVRSASVKPMRNRRQKRVEARVADESGPMVAVWFNQP
                     WVADRLAPGSRVLLHGRMKRRNQFWVDSWEAVDGTDGGVHTLGHVAKYAATRGVTEQQ
                     LRTAVHEALERVHDVVDPLPSALLRAERMPRRAAALAAIHFPADDEEREAARRRLAFE
                     ELLLLELALAGRKRARDEELEATVLTGDGALVEPWLAALPFEPTHDQRDAFARIDYDL
                     ERERPAQRLLMGEVGSGKTVVALYAMLRAVESGSQAALMAPTETLAEQHLLTIDRLLG
                     GLVPVALLTGSTPAARRREILARLASGELGLVVGTHALIEPVVEFRSLGLVVIDEQHR
                     FGVRQRAALDAKAPAGRVPHALHLTATPIPRTLALTLYGDLDVTTLRELPAGRKPVET
                     YVVDGARPRARAYERIREEIASGRQCFVVCPLVEESEALQAKAATAEAERLARTEFRE
                     HTVELIHGQMPASKKAEAMRRFAAGEADVLVATSVIEVGIDVPNATVMLIEAAERYGL
                     SQLHQLRGRVGRGKHESLCILFGDPELPRLKAIATERDGFRLAEIDLELRGEGQILGT
                     RQHGLPEFRVARLPEDLRLLESARDHAEQIVAADPRLEQPEHVLLRDAAAERFGPERA
                     PIPA"
     gene            complement(8609..10246)
                     /locus_tag="VF752_11675"
     CDS             complement(8609..10246)
                     /locus_tag="VF752_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX8856248.1"
                     /translation="MPDPSLERFRSAVAAAYAELESRRVEVNDLNVFPVADGDTGDNM
                     AMTMRAVLDELDRMNGEPIDVIGREEIVHAVARASLLGARGNSGVILSQIVRGAAAEL
                     VSRPGELVDPVLVAAALARAADAAYASVRDPAEGTMLSAIRAMAHRVAQELAHMDTPR
                     LNGNVAPEEQDELLAEVLERALEAGKEAVERGREQLPALRDADVVDAGAYGLTVIVAG
                     VIGALRGQAPPELEHQEAPRRSLHLPQHESSKYRYCTNFAVSGSGLENQRFVPMLEEI
                     GDSVLVVGDDETLKVHVHTDDPDRAIALFAAHGQVSRFDIADMREQVADRTARLGRRA
                     GTDTCGVVAVANGAGMLQMFEGLGALVVDGGQTLNPSTYEILARIHDTDAEEVVVLPN
                     SANVIMAAERAAELSEKRARVVPTRAQQAGLAALIAFDSRADAAANAVALEEELGRLR
                     SGGIARAARDDARGRFREGDALGYIGDDLVAWGDVRETVAATFEIFCEDAEVLTCIAG
                     EGAPLGEDEIAELVPGEIELEQHDGGQPAWWWLLCAE"
     gene            complement(10298..10489)
                     /gene="rpmB"
                     /locus_tag="VF752_11680"
     CDS             complement(10298..10489)
                     /gene="rpmB"
                     /locus_tag="VF752_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX8856249.1"
                     /translation="MAKVCYNCKKGPAFGHSRSHSMVATKRRFEPNLQKVRINVNGAP
                     RREYVCTRCLKAGKVTKYV"
     gene            complement(10501..>10741)
                     /locus_tag="VF752_11685"
     CDS             complement(10501..>10741)
                     /locus_tag="VF752_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856250.1"
                     /translation="DAWIHGDTREQARALGDRLWILNHGTNAWFPDDLVELIRQEVPE
                     AVFVDIEDGPITRPDITADAIRRAVAAAGVTARGC"
BASE COUNT         1470 a         3873 c         3751 g         1647 t
ORIGIN      
        1 cgacggcggc ctcgacctcg ccgcggcgct cctccgccgt gtcgcgctcc gacgcgagct
       61 tggcgatctc ggcctcgagc ccggcggtgc gctctgcctc aacggcgacg acctcgcgct
      121 cgagctccgc gatacgcgcg gagtgctcgc tctcgcgcgc ctcgagcgcg cccgcgacgc
      181 ccgcggcgcc gccctcgctc ggatcgccgt cgcgctctac gagcgcctcg acctcgcggc
      241 gacggcgcac ggcgccctcg gcgccagccc gcgcgatctc gcgggcgcgc tcgagccgta
      301 gcccgatcct gtcgaccgcc gagcgagcgg caaacgcccg cgccgacagc gcctcgcgcg
      361 cgcggctgtg ctccgcgaac gcctcttctg ccgcctcgcg ccggcgcgcc acctcggcga
      421 gcgacttctc cacctcgtcg cgccgcccgg cggcggccgc ggcgacggcc tccgcctccg
      481 ccaacgcagc gatggcgccg cgcgcacgat cacgcgccag ctcgagccgc gcctcgagcg
      541 actgccgctc gagccgttcg tgcaattcgg cggcctccgc ctggcgcttc agcggccgca
      601 gtcgcgagcg cgcttcgcgc tccacgtcga gcgcgcggtc gagattgtcc tgcgtgcgct
      661 cgagcttcag ctgcgcgcgg cggcggcgct ttcggtgctt gccgaggccc gcagcctcct
      721 cgatcagcag gcggcgatcg cgcggcttcg agtggatgat cgactcgacc cgcccctgcg
      781 acaccaccga gtgcatctcc ttcccgaggc cggtgtccga cagcacctcg agcacgtcga
      841 cgagccggca gcgggcgccg ttcaggcggt actcgccgtc gcccgagcgc tccagcctgc
      901 gcacgatcga gatctccgag aactcggagt cgatgcctcc gtcggagttg tcgatcacca
      961 cctcgacctc ggccgagttg cggctggcga tcccgtgccc gcccgcgaag atcacgtcct
     1021 gcatcgtctg gccccgcacg gcaagcgggc tctgctcgcc catcgcccag agcacggcgt
     1081 cggtgatgtt cgacttcccg gagccgttcg ggccgacgac gaccgacacg ccgggcgaga
     1141 agtcgagccg ggtccggtcc gggaacgact tgaagccctt gagcgtgagc gagcgcaggt
     1201 gcaccggcct agccctcctg ctcctgggcg agcgagtcga gcgcgcgcag ggcggcctcc
     1261 tgctcggcac tcttcttggt acgcccctcg ccgcggccga tctcgcgccc cgacacctcg
     1321 gcgaccgcca cgaagctgcg gtcgtgcggc ggaccggcct cgtcgtcgat ccggtacgcc
     1381 accacctgag cgcgccgcgc caggcgctcc tgcaggacgg acttgtagtc gacggggtgc
     1441 tcgagtgcct cctcgacctg ctcggcgaag gcgtatacca ccgcgggcgc cacgcgctca
     1501 aaaccgaagg cgagataggt ggcgccgatg atcgcctcgc agatcgaggc gaggatgcgg
     1561 tcggaggcga tcagcatctc cgcgctctta cccgtgccct ccggtgccgc ggcacgaagc
     1621 cgctcgggta cgccgagatc gcgtgcgacc tccgcgcatg cgtggcgcga aaccgcctgc
     1681 gcccgcagct tcgttagccg gccggcgccg taacgcggga agcgcgggta gatgtcggtc
     1741 gagatcgcga ggctcaggac gacgtctcca aggaaggcga gccgctcgta ggactcccag
     1801 cgattgtcga cccacgacga gtgggtgaaa gcctgccgtg cgaggtcatc aggtagctcg
     1861 tcgagcagtg agctgagcgt gtccagtggt cagggagcgt tctggggcgc gtgcgcggcg
     1921 acgaagtccg ccgcctgccc gacggtgagg atcttcgcgg cctcctcgtc gggaattcgg
     1981 accccgtagt tgtcctcgag ctccatcact agctcgacca ggtcgagcga gtcggcctcg
     2041 aggtcctcct tgaagcgggt gtcctcgccg atcgaggagg ggtcgcgcac gagttcggtc
     2101 gcgagatggg cgcggatgcg ctcgaggacc tcttccctag tcatgagcga cttcgacgct
     2161 atcaacgggt gcggaccgca aggccccgcc ggcctcgagc gcggccgcca tgcgaccgac
     2221 ggcgtcctcg cgcacggccc gctccgcgag ccgcacagcg ttggcgatgc cgtcggcgct
     2281 cgagccgccg tgggcgatca cgactgggcg ccggaggccg agcaggatcg cgccgccggt
     2341 ggcgttcggg tcgagcgcat ctcgcagggc gctcacgcga cggcggatca gcaggccgcc
     2401 gaggctcgag accgcgccgg agcggacggc gtcgcggatc gcgccgacca cggtcttcgc
     2461 agtgccctcc atgagcttca gcgcgacgtt gccggtgaag ccatccgtga cgaccacgtc
     2521 ggcggcgccg gccggcacgt cggtgccctc gacattgccg atgaactcga ttccggacgc
     2581 tcccgcgagc cgctcgtgcg ccgccaccac atcgggcgtg cccttcgtcg gctcctcgcc
     2641 gaccgacagc agcgccaccc gcggacgctc caccccgtgc atcgtctgca tgaacgcgga
     2701 gcccatgaac gcgaactgca cgagatgctc aggccgcacc tcgaggttcg cgccgacgtc
     2761 gagcatcagg gtcggcgcac cgggaatcgg gagaaacacg gcgacgcccg gccggtgcac
     2821 cccctggagg cgccggatat gaagcacgcc ggcggcgagg gccgcgcccg tcgcgcccgc
     2881 cgagacgagc gccgagcagc gcccctcggc cacggccttg gcggcctgca cgatcgacgc
     2941 gtcgggcttc gagcgcactg cgcgcacagg ctcctcgtcg ttcccgatcg cgaccggggc
     3001 gtcgaccacc tcggcgccat cccgcagctc ggcggccggc ccgaacagcg tgacgggcac
     3061 gcccgagcgt cttccgccct cggcgacggt ccgcggaccg ccgtccgcgc cgttcgcgtc
     3121 gagcgcgatc accggcgcgg cctactcgtc ggcgccagag cccgacggag tgaatatctc
     3181 gcggcctccg taggtgccgc agaccgagca cacgcggtgc ggcaggcgcg gcgagtggca
     3241 ctgcgggcag gtgcccagtg ccggaggcgt gatcttgtgc tgcgaacgcc gcttgttggt
     3301 tcgcgagtgc gactgcttct gcttgggaac ggccatgcgg cgggcaagtc tagcgggggc
     3361 cgcgcgagag ctcgcagagg ccggacgcgg acccggccgg cttacctact cgaagcgaag
     3421 ctcgccgagc ttcgcccagc gcgggtcgcg ggcttcctcg tgccgatggt cggcacccgc
     3481 gctgttcagg ttctctccgc acaccgggca caacccgagg cactcgtcgg tgcacagcac
     3541 ctgcgccggc atggcgaaga tcaccgcgtc gcgggtccac gagcgaagat cgaggtcgtc
     3601 cccctcgacg tacggcgagc gcagctcgtc gtcgccgccc ggctggtcga cctcgcgcac
     3661 atcgacctcg acggagcgct cggcaggctc gaggcaacgc acgcacgctc cgaccagcgt
     3721 gacgttcgtg cgcagcctga gcgagtagcc ggtggtggtg tgcgacacgt ccagcacgac
     3781 gtgcgcgacg cggtcctcaa cgccatactc ctggccgccg aggtcgatcg ggtcgacgtt
     3841 cacagcggtc tcgatccggc gcccctcgcc cgagtgaaga ttgaggcggc ctagatcgaa
     3901 gatgtccgtt cgctgcccca tgaattcagg gtagcgccgg tctcgagccg tagttcggct
     3961 gcgaagcatc agctggcggc gtcctcggag ctggccaggc ggagccgtcg cgccgctgcc
     4021 ctcagcgtcg cctccacgtg atccggttcg tacctcacct gcgcgtgcgt gaatcggagc
     4081 ggagtgagct ccgccgcgaa gtgcttctga tccctgagcg cgtcgcgcga ctgctgcgcc
     4141 ggtgtgcggt gataccggag gccgtcggtc tccactacga ggccgagctc gggccagaag
     4201 aagtcgacct tgaagccgtt gacgagctgc tgcgtcttgg gcatcgggag ccccgctctg
     4261 cgggcgatgg ggaggaagcg gcgctccagc tccgagtcgg tgagggcgaa ggtgcggcgg
     4321 tcgagcagcc tgcggacgcg cgtgacgcct gggcgcttgc cgtggtggtc gagcgcagca
     4381 cgcagcgctt cgggatccgc taggtcgagc ttgtcggcct cgttgacggc cgcctcgagc
     4441 tcgttgtcgc tgagcgtggt cgcagcgagg tcgatgagcg tgcagatcgg ggttgtgacc
     4501 gggatgccgt ggcactccgt gacgtcgtcg tccgaaaacg cccgccggcg gtggatgttg
     4561 atgccggcgc ggtgagatct cctggccgcc gggacgctga tttcaattcc ccgtaattgc
     4621 tgctttcgga tgcgccagag aaccgcggcg ctgtggtggc tcaacgcggc gcccgctccg
     4681 caggcgagga ccgcagccat ccacacgccg tactgagaca gctcccgccg gcctaccgcg
     4741 tagacgccac gcgcgactcg gtgcaggcgt ccctttttcg cacgatggtc gatcgcatga
     4801 gcactaagac catgctccaa gagctgatct cgggtcacca cgccgtgctg gctcctgacc
     4861 agcttccaaa tctgcgatgt gtggaacttt tgcccggcta cagccgggta aagggttcac
     4921 agatttggga ttcgcccccg ctgggggcct gcgactagcg gacttcggcc tcttcgcgcc
     4981 cggccaggcg ctcgcggccg cgttgcaccg ccgcggtgaa cttctgcagg ttcacctcga
     5041 gcgtgttgag gatgtcgtcg gcgtagtcct cggcgccgag gcggatctcg cgctcgcgcc
     5101 cgcgggcctc ttcgatgatg tcctccgcct ggcgctcggc ctggcggacg atctcctcct
     5161 gcgcgatcag gcgggcctgc tcgtcgcggg cctccttgat gatgcgctcg gcctcgcgct
     5221 tcgcctcggc cagcatctcc tgacgttcct tgacgatcca gcgcgcctgc ttgatctcct
     5281 cggggatcgt ggcgcgcatc tggtcgagga tgtcgtagat ctcctccttg tcgacgcgaa
     5341 cctggtccgt cagcgggacc ggcttcgcgt tgtggatttg atcgtcgagt ttgtcgatga
     5401 ggaccaggac gtccatgtga acctcagttt cgtcagcgct tgagcgcttc ctctagccgc
     5461 tgtgccacct gcggcggcac gaggtcggtg aggtcgccgc cgaacatggc gagttctttc
     5521 actccactcg agcttaggaa gctgtactgc ggtgaggaca tcatgtagat gctctcaatg
     5581 tcgggcgcca tcttgcggtt gagctggttc atctcgaact cgtattcgaa gtccgagatc
     5641 gcccgcaggc ccttgacgat ggccttggcc ccgtgctgcc gggcgaaatc gacgagcagc
     5701 gtatcaaagg atttcacctg cacattgccg agttttccga cctcggcctc gatgaaagac
     5761 acgcgctcgt ccgcggtgaa cacggtgctc tgcttgcgca cgggctggtt cacgacgccg
     5821 acgacgaccg tgtcgaacac ctcggcagtg cggcggatga tgtcgaggtg accgaaggtg
     5881 accgggtcgt aggagcccgg gcagaccgcg gtgcccttgc cgtcgtcact gaacacgatg
     5941 cagggcgatc cgcgtgtcgc cgtaggtgcg ctcgtcctcg agcggcagcg ttagctccag
     6001 cgggttgcgc ttgtctgact cggacacgat gaggcctctc ccggacgcta cggctggcag
     6061 ccgctccgaa agcggtcccg ccagccggtc ggcggaacta tacggagggt cgaggaaaac
     6121 gaggtcatat ggggcgcccg gcgcgcgcgc cggagccgcc aggaacgcga gcgcatcgcg
     6181 ccgtatcacc ggcgcatcga ggccgagctc ggcgaggttg cgcctcaccg ccgcgatcgc
     6241 gcgtgtgccc gaatcgacga acgtggcgga tgtggcgccg cgcgagagcg cctctatccc
     6301 gagcgccccg gagccggcga acaggtcgag caccgcgagc cccgatacgt cgccgaggat
     6361 cgagaagagc gcctcgcgca cgcggtcggc ggtcggccgg gtgccccgtc ccggcggcgc
     6421 atgaaggcgc ctgccgccga gcgagcccgc cacgatcctc atgcgggtat cggcgcgcgc
     6481 tccgggccga accgctcggc ggccgcgtcg cgaagcagca cgtgctcggg ctgctcgagc
     6541 cgcgggtccg cggccacgat ctgctccgcg tggtcgcgcg ccgactcgag caggcgcagg
     6601 tcctcgggca ggcgggccac ccggaactcc ggcagcccgt gctggcgcgt gccgaggatc
     6661 tgcccctcgc cacgcagctc gaggtcgatc tccgcgagcc ggaagccgtc gcgctcggtc
     6721 gcgatcgcct tgagccgcgg cagctccggg tcgccgaaga ggatgcagag cgactcgtgc
     6781 ttgccccggc ccacgcgccc acgcagctgg tggagctgtg agaggccgta gcgctcggcc
     6841 gcctcgatca gcatcacggt cgcgttcggg acgtcgatgc cgacctcgat caccgacgtc
     6901 gccacgagca cgtccgcctc gcccgcggcg aagcggcgca tcgcctccgc cttcttcgag
     6961 gccggcatct gcccgtggat cagctcgacc gtgtgctcgc ggaactcggt gcgcgcgagc
     7021 cgctccgcct ccgcggtcgc ggccttcgcc tggagcgcct cagattcctc gacgagtgga
     7081 cacacgacga agcactggcg gcctgacgcg atctcctctc gaattcgctc gtacgcgcgc
     7141 gcccgcggcc gggcgccgtc gaccacgtag gtctcgaccg gcttgcggcc ggcgggcagc
     7201 tcgcgcagcg tggtcacgtc gagatcgccg tagagcgtga gcgcgagcgt gcgcgggatc
     7261 ggcgtggccg tcaggtgaag cgcgtgcggc acccggccgg ccggagcctt ggcgtcgagc
     7321 gcggcgcgct gccgcacacc gaagcggtgc tgctcgtcga tcaccacgag ccccagcgag
     7381 cggaactcga cgacgggctc gatcagcgcg tgcgtgccca ccaccaggcc gagctcgccg
     7441 cttgcgagtc tcgcgaggat ctcgcggcgg cgcgccgccg gcgtagagcc tgtcaggagc
     7501 gcgacgggca ccagtccgcc gagcagccgg tcgatcgtga gcaggtgctg ctcggcgagc
     7561 gtctccgtgg gagccatcag cgccgcctga gagccgctct cgaccgcgcg cagcatcgcg
     7621 tacagcgcga ccaccgtctt gccggagccc acctccccca tcagcagccg ctgcgccggc
     7681 cgttcgcgtt cgaggtcgta atcgatgcgc gcgaacgcgt cgcgctgatc gtgcgtcggc
     7741 tcgaacggca gcgcggcgag ccagggctcg accagcgcgc cgtcgccggt gagcaccgtc
     7801 gcctcgagct cttcgtcgcg agcgcgcttc cgtcccgcga gcgccagctc gagcagcagc
     7861 agctcctcga acgccagccg gcggcgcgcg gcctcgcgct cctcgtcgtc cgcggggaag
     7921 tggatggcgg cgagcgccgc ggcgcgtcgg ggcatccgct ccgcgcggag cagcgcgctc
     7981 ggcagcgggt cgaccacgtc gtgcacccgt tcgagcgcct cgtgcacggc ggtgcgcagc
     8041 tgctgctcgg tcacgccgcg ggtggcggcg tacttcgcca cgtggccgag ggtgtgcacg
     8101 ccaccgtcgg tcccgtcgac cgcctcccag ctgtcgaccc agaactggtt gcgccgcttc
     8161 atgcgcccgt ggagcagcac gcgagagccc ggcgccaggc gatccgcaac ccacggctgg
     8221 ttgaaccaca ccgcgaccat cgggccgctc tcgtcggcga cgcgcgcctc gacgcgcttc
     8281 tggcggcggt ttcgcatcgg cttcaccgac gccgagcgca cctgtgcctc gaccgtggcc
     8341 tcttcctcga tgccgatcga ggcgatcgtc cggcgctcgc gcaggtccgt gtgcgcgcgc
     8401 gggatgtgct cgagcagctc gccgaccgtc gtgattccga tcgccgcggc aggctcgcgc
     8461 gcacgcttcg gcagcggcgg ctcctcggcc agccgcaacg ggcgcggcca cgagcgcacc
     8521 ggcgcctcga gggcgtcggc ctgctccagc tcagtcgatt ctgcgaagtg agtgggaatc
     8581 gaccgtattt acgcaggcgg gcggacgttc actccgcgca gagcagccac caccatgccg
     8641 gctggccgcc gtcgtgctgc tcgagctcga tctcgccagg cacgagctcg gctatctcgt
     8701 cctcgccgag cggggcgccc tcgcccgcga tgcaggtgag cacttcggcg tcctcgcaga
     8761 agatttcgaa cgtggccgcc acggtctcgc gcacgtcgcc ccaggcaacg aggtcgtcgc
     8821 cgatgtagcc gagcgcgtcg ccttccctga agcgcccgcg ggcgtcgtcg cgcgccgcgc
     8881 gggcgatgcc gccggaacgc agccggccga gctcctcctc gagcgcgacg gcgttcgcgg
     8941 ccgcgtcggc gcgcgagtcg aaggcgatca gcgccgcaag gccggcctgc tgcgcgcgcg
     9001 tgggcaccac gcgggcccgc ttctcggaga gctccgcggc gcgctccgcc gccatgatca
     9061 cgttcgcgct gttcgggagg accacgacct cctcggcgtc cgtgtcgtgg atgcgcgcga
     9121 ggatctcgta ggtggaggga ttgagcgtct ggccgccgtc cacgacgagc gcgccgaggc
     9181 cctcgaacat ctgaagcatg ccggcgccgt tcgccacagc gaccacgccg caggtgtccg
     9241 tgccggctcg ccgcccgagc cgcgcggtgc gatccgccac ctgctcgcgc atgtcggcga
     9301 tgtcgaagcg cgacacctgt ccgtgcgcgg cgaacagcgc gatcgcacgg tcggggtcgt
     9361 cagtgtgcac gtgcaccttg agcgtctcgt cgtcgcccac cacgagcacg ctgtcgccga
     9421 tctcctcgag catcgggacg aagcgctggt tttcaagccc ggagccggac accgcgaagt
     9481 tcgtgcagta ccggtacttg ctcgactcgt gctggggcag atgaagcgag cggcgcggcg
     9541 cctcctggtg ctcgagctcc ggtggcgcct ggccgcgcag ggcgccgatc acgccggcca
     9601 cgatgaccgt gaggccgtag gcgccggcgt ctaccacgtc cgcgtcccgc agcgccggca
     9661 gctgctccct gccgcgctcg acggcctcct tgccggcctc gagcgcccgc tcgagcacct
     9721 cggcgagtag ctcgtcctgc tcctccggcg cgacgttgcc gttcaggcgc ggcgtgtcca
     9781 tgtgcgcgag ctcctgcgcc acgcggtggg ccatcgctcg gatcgcggag agcatcgtgc
     9841 cctcggccgg gtcgcggacc gacgcgtagg ccgcgtcggc ggcgcgcgcc agcgccgccg
     9901 ccacgagcac ggggtcaacc agctcgcccg ggcgcgacac cagctcggcg gccgcgccgc
     9961 gcacgatctg cgacaggatc acgccgctgt tgccgcgagc gccgagaagc gacgctcgcg
    10021 ccacggcgtg aacgatctcc tcgcggccga tgacgtcgat cggctcgccg ttcatgcggt
    10081 cgagctcgtc gagcaccgcg cgcatggtca tcgccatgtt gtcgccggtg tcgccgtcgg
    10141 cgacggggaa gacgttgagg tcgttgacct ccacacgccg gctttcaagc tcggcgtagg
    10201 cggcggccac ggcgctccgg aagcgctcga gtgacgggtc aggcacgaag atgttctaca
    10261 ccacgtcgcg gcgcgggtaa cgccgggcgt gatggcgcta cacgtacttg gtgactttgc
    10321 cggccttgag gcagcgtgtg cagacgtact cgcgccgcgg cgctccgttc acgttgatcc
    10381 gcaccttctg gaggttcggc tcgaagcggc gcttggtggc gaccatggaa tggctccggc
    10441 tgtgaccgaa cgccgggccc ttcttgcagt tgtaacagac ctttgccacg gcgcggaagg
    10501 ctagcaaccg cgcgcagtga cgccggcggc ggcgaccgcg cggcggatcg cgtccgcggt
    10561 gatgtccggc ctggtgatcg ggccgtcctc gatgtcgacg aagacggcct cgggcacctc
    10621 ctggcgaatg agttccacaa ggtcgtcggg gaaccatgcg ttcgtgccgt ggttgaggat
    10681 ccagaggcga tcccccagag cgcgcgcctg ctcgcgcgtg tcgccgtgga tccaggcgtc
    10741 g
//
LOCUS       DASVXS010000209        21177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_977360, whole genome shotgun sequence.
ACCESSION   DASVXS010000209 DASVXS010000000
VERSION     DASVXS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21177
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977360"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF752_11690"
     CDS             <1..243
                     /locus_tag="VF752_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8856251.1"
                     /translation="LAVSPHHLSRTFRALTGHTVSRHRMRLRARAAMERMAAGDGDLA
                     RIAADLGFADQSHLCRVMRAETGRTPSALRRVLAAE"
     gene            658..1344
                     /locus_tag="VF752_11695"
     CDS             658..1344
                     /locus_tag="VF752_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX8856252.1"
                     /translation="MTKFEGFGPDVRKWFQGLEADNSREYFVASRDFFEESIRGQMEA
                     LLTELSEKFGGEVKMFRQNRDIRFSPDKSPYKTNTYGVVYGSGMAAQGLYASISAGGL
                     VAGSGYHVMARDQLDRYREEVANGKHGQELTKLVAKAERAGLELWGESLATAPRGYPK
                     DHERIELLRRKNLTLGATLKFARGISRSDGLQFVAKTWRAAAPVTGWLDEHVGASTMP
                     VRRPARRRRT"
     gene            complement(1361..1888)
                     /locus_tag="VF752_11700"
     CDS             complement(1361..1888)
                     /locus_tag="VF752_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856253.1"
                     /translation="MPTFELQFPASGIEALASRFPHADDGRLLALGAAARARGYYSRA
                     EFIEVCAGKTVRSRAKVASNTEAAVIEATGRALTTSEQEAARMAALLGLEGVGVPTAS
                     TLLYVAFPNDYPILDVRALESLGVKPRSQYPVTFWLEYLNACRELAQRYGVSLRTLDK
                     ALWQHSKERPDMSSA"
     gene            2057..3082
                     /gene="lgt"
                     /locus_tag="VF752_11705"
     CDS             2057..3082
                     /gene="lgt"
                     /locus_tag="VF752_11705"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX8856254.1"
                     /translation="MVAVIPSPSSNGFHLGPLLVHAYGLAYVVAVLAAVVLTTRRWEV
                     RGGSRELVYEVALFGFPAGLIGGRLYFLATSWNEVPDHWWGPFAIWKGGLGIWGGIAL
                     GTLAGVWVLHRRRADIPRFLDAAAPGLLVAQAIGRVGNYFNQELFGGPTNLPWGIHID
                     PANRPAGYAHYTTFQPTFLYELIWNLALAAALIWLGRHRQIRAPGLFALYVAGYSGFR
                     IFEELLRVDPAHHILGLRLNFFVAVLLFAAGLAWFARIQRGGATRRLVSRGGPAALGV
                     AWLACVLAGCGQAHRPETASSAPAPLLRASTAGREPTTPRQLLVVDLSQMRTAMRRAD
                     PVHPPGP"
     gene            3432..3830
                     /locus_tag="VF752_11710"
     CDS             3432..3830
                     /locus_tag="VF752_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856255.1"
                     /translation="MSEKNVETVRRVYEGVNARLEVPRELFDPAYQFDNTELWPDIGE
                     VLGFEAAQEAMREYWETFEAYHVEIDEVIYADEGRVVDVVRDGGRMRGTDAEVWNRFF
                     HVWTLRDGRIVRLSIHTDRNRSLEAAGLSE"
     gene            3985..5628
                     /locus_tag="VF752_11715"
     CDS             3985..5628
                     /locus_tag="VF752_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8856256.1"
                     /translation="MAATGEQFLREGGEAIAAADWERARACFEEARELDGAPEALDGL
                     SEVANFEGDYERAIELKEAALAAYRARDQRVEASHVARWLAFMHATYHGNYAVASGWM
                     ARAESLLDGVEECAAHGWLILDRAPFSRSPQERQQVAASALAIARRFGDAELEFEAMA
                     LLGESRVALGRIDEGMKLLDEAMAALAGGEIADHKAIGEIYCRLLSACEQATDVRRAE
                     DWMSAIDRHVVWTDFVRPTCRTHYGGILMALGRWQEAEAELLAAIKTFERGYRGDGAF
                     PLVRLAELRVRQGRFEEAERLLENREWHPLARRAAAAIALGRRELPLAEELTRLCFEG
                     AADDDPACGPALVLLVAVQLERGDEEGARSTLDRLAGLAASCGSQSLEASVELSAGRL
                     AILRGDDAAIGHLKRAVERFAGLELPFETARARLELARALAPTRPDAAAYEARLALDA
                     FERLGAAHDCDAAAELLRTLGASGRAWPKGRGPLTKRETEVLALLVEGLANAEISERL
                     FISVRTAEHHVASILAKLDLRSRAEAAAYGVRQGAAKDQ"
     gene            5728..6147
                     /locus_tag="VF752_11720"
     CDS             5728..6147
                     /locus_tag="VF752_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8856257.1"
                     /translation="MSDSEQNKAAVRDCFAEASRGNFGALDSILSPDYVLHPEGVRGS
                     EGLAEMVQGYREAIGGLTVTIDQQFTEGDYVATRFTIRGRHEGELMGTEPTGRDVEFA
                     GLTVSRCRDGRIEEEWELVDTVSLLKQVGALPEVAQA"
     gene            complement(6374..6506)
                     /locus_tag="VF752_11725"
     tRNA            complement(6374..6506)
                     /locus_tag="VF752_11725"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6411..6413),aa:Val,seq:cac)
     gene            complement(6879..8600)
                     /locus_tag="VF752_11730"
     CDS             complement(6879..8600)
                     /locus_tag="VF752_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX8856258.1"
                     /translation="MLGDPDPALAVQQLWDPQRFDEVIVSTLPARISPWLKLDLPRRV
                     ERITGRPVRHVTASQGHQGEAAGGRAPTESGEGGPMSDQSPKVPSLRLQPSASYSFTM
                     RLHMPQQGGAFAHVAEAIAESEALLGAIDLVRVERQEVVRDVTVACADAGHAEAVVRA
                     VRALEGVRVDSVSDRTFLMHKGGKIEVNAKLPLKTRDDLSMAYTPGVARVSTAIHEDP
                     GKAWALTIKSNTVAVVSDGSAVLGLGDIGPAAAMPVMEGKAMLFKEFAGVDAFPLCID
                     LEEVDEIVAFVRAVAPTFGGINLEDIAAPRCFEIERRLRAELDIPVFHDDQHGTAIVV
                     LAALLNALRVVGKRPEDVTVVVVGAGAAGVACTEILLAQGVGDVVVCNRRGALYAGAE
                     DLDAERASLAERTNKRGLRGKADEVLAGAHVLLGLSGPGAVSPAGVRAMAPDAIVFAM
                     ANPVPEVHPEEVRDDVAVMATGRSDYPNQINNVLAFPGVFKGALEVRARTINEEMELA
                     AAHAIAHVIPEQELHADYIIPSVFNRHVAESVADAVAGAAVASGVARRERRVHPPEPA
                     TPLEALR"
     gene            8975..11113
                     /gene="acs"
                     /locus_tag="VF752_11735"
     CDS             8975..11113
                     /gene="acs"
                     /locus_tag="VF752_11735"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX8856259.1"
                     /translation="MGVEDQAPSTAEVGEAGLSEAQIAVHWREEEYYPPPETFVVQAN
                     ANDPSILERFGEEHFPDCFVEYAEMLDWDTRWETVLDTSNPPFWKWFVGGRLNASVNC
                     LDRHLESRGSRNALIWVPEPEDEQTQYITYEDLRRRVNEFAALLRGFAGVETGDRVTF
                     HLPMVPELPVSMLACARLGVIHSEVFGGFAGTACGHRMADSQSKILVTMDGYYRNGEL
                     IDHKSKADEAIAAAREEGVEVEKVLVWRRHPDAYASNSPMVDGRDFFIDELLKDYEGN
                     TVEPASMPAEAPLFLMYTSGTTGRPKGCQHSTGGYLAYVTGTSKYYQDIHPDDVYWCF
                     ADIGWITGHSYIVYGPLSLGTTSVMYEGVPTYPDAGRPWRIAEKLGVKIFHTAPTTIR
                     MLRKLGPDEPAKYDYHFKTMTTVGEPIEPDVWRWYYQVVGKGEAAITDTWWQTENGGF
                     LGSTLPGLQRMKPGSCGPGVLGIYPVIYDEEGNEVPKGSGKAGNICIRNPWPGIFQTI
                     WGQPERFVDTYYTKYCKDRGSTDWRDWPYFAGDGAVQAADGYFRILGRVDDVINVAGH
                     RLGTKELESAAIMVDEVAEAAAVPVIDEVRGKIVEMYVALKPGFQPSDEIAQKVSQTI
                     VKEIGAIARPKNVWIVADMPKTRSGKIMRRVIAAVSNFADVGDVTTLANPEIVQQIRH
                     HVQSEKLAHGEVPRELSEKERQEIAAYGAE"
     gene            complement(11128..11406)
                     /locus_tag="VF752_11740"
     CDS             complement(11128..11406)
                     /locus_tag="VF752_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856260.1"
                     /translation="MTQQYLIGQLSALLNELQPPTGARLADAVCELRREVERCPLRTL
                     PKLAHEAMDLTDVICWDALERGDADFGRYARAAAALGEFADNAGWMPE"
     gene            complement(11569..11640)
                     /locus_tag="VF752_11745"
     tRNA            complement(11569..11640)
                     /locus_tag="VF752_11745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11606..11608),aa:Val,seq:cac)
     gene            11709..12527
                     /locus_tag="VF752_11750"
     CDS             11709..12527
                     /locus_tag="VF752_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856261.1"
                     /translation="MAADRGAISVNGADIAWMAEGAGPDVVLIHSAIADMRMWDELVG
                     RLRDRHRVIRYDMRGWGESPRPPGRYSHIEDLAALLTELGDGPATLVGCSLGSRVAVE
                     FALEHPDAARALLLLSPFLRGHKWTPELGQFWAERRQDLDNGDIDAAVRLNVEQWAGG
                     LPEEQRQLIADMQRRAFELELAVDAQQDGFEPPAAQRLGELRLPTLVAVGERDFSDFH
                     AVADRLARGIAHSRSAMVPGAGHLLPMERPRETAGLLEELERAPTSAIERGPQM"
     gene            12536..13111
                     /locus_tag="VF752_11755"
     CDS             12536..13111
                     /locus_tag="VF752_11755"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX8856262.1"
                     /translation="MTRYDAYLFVHVAATIIWVGSGSLLTVLAYRTERANDEQRIRNL
                     LDDSNALGTRLFIPAALTVVAFGILMVVDGPWTFSYLWIVLGLVGYAATSVTGAFVIK
                     PWSERIAELIKREGSVTPAAMAEIRRLLWFGRIDLLTLFLVIFDMAVKPSGDDTAILV
                     GMALVFLAGAAFVASRIRGVEEAPEPQVATG"
     gene            13134..13401
                     /gene="ffs"
                     /locus_tag="VF752_11760"
     ncRNA           13134..13401
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF752_11760"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            13438..13998
                     /locus_tag="VF752_11765"
     CDS             13438..13998
                     /locus_tag="VF752_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8856263.1"
                     /translation="MLQTLRIPPLTACVLAAAVLTGCGSSGGGAHKPLPPPGNIKVTS
                     SAFAQGGTIPKRYTCDGAGLSPPLAWRRLPDATRSLVLVVLDPDAPGPTFFHWVIYDF
                     SPNQREVAAGESPRGAAAGENSAGKAGWTPPCPPKGNSPHRYVFRLFADSKPTDLAAG
                     AAPSDVLAAAGTHSIAQGTLTGRYGR"
     gene            complement(13986..15446)
                     /locus_tag="VF752_11770"
     CDS             complement(13986..15446)
                     /locus_tag="VF752_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856264.1"
                     /translation="MHPSLGPRRPRGGGLLVDAVAAAVVAAVVWAWFGHAFLNYDTFY
                     ALLWGHDLAHGLKPQYEVPVAPTPHPLAELVGLVLSPLGDGAENAMLGVILFAFGWLV
                     VGIFRLGEELFSWPVGLLAALLVATRVPYLNFAIRGYVDFAAMAFIVWAGVLEARRPR
                     RGWPVLVLLGLAGLLRPEAWLFIAAYWLWMFPARDWAGRIRLALLAAAAPLIWGFSDL
                     AITGDFLWSLNGTSDLAQQLGRQTGLRHVPVGARRLGEILRLPELIASVIGFVVGWRV
                     FGRRMAVPTSIAVLNGVAWIVFAIAGLSLLGRYLFLLGTMLALFAAVAALGWTALEPS
                     HPWRRAWSIGGSAIVALVVLFFAVQQVDRLKSLRTDIGNRDRIQADLHGLASRAPVKS
                     LVRGCRPTYVPNHRPVPLIAYWDDIRPASIVTRQPGAAASRGTMFFPASAEVEKLSIL
                     DPHEPTVIQLRVPPGWREAARNRSWVLYAGPACQRP"
     gene            15453..17126
                     /gene="dnaX"
                     /locus_tag="VF752_11775"
     CDS             15453..17126
                     /gene="dnaX"
                     /locus_tag="VF752_11775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX8856265.1"
                     /translation="MLSLYRRHRPRTFDDVVGQEHIVRTLRNAIELDKVHHAYLFVGS
                     RGTGKTSMAKLLASALNAPGGPKADFDPDDPSAAAIRNGTSLDVVEMDAASNNSVDDI
                     RELRENVALAPMGGSRRVYILDEAHMLSASAWNAFLKTLEEPPPHVVFVLATTEAHKV
                     PPTIVDRCHRFDFHRPSLEQIVGVLRKVSAEEGIDVPDQALVMIARSATGSFRDALGT
                     LEQLVTYGGKNVRLEDVLEVLGVADAELVLEAAQAIADHDAREALLAVERLSASGRDL
                     VQFMRDLAAHLRHLFVVQTLGEVPESFAVTAQHTDRLQAQAEALAQGEILRAIDLLAA
                     ALSAVKDGSEPRIQLELALLKAAHPESDVTLDALTRRVERIEKSLGGGPAPAPAAPEA
                     ARPPEVAPEPVRAAQPDPEPEPQPDPEPEPEPAAEPASDLGLDRVRELWPAAVDAVRV
                     ENAMVGALLAEARPTALEGERLTVTFPGDSTFSKKKAESNRVLLHDALHGLTGRSLTL
                     TYELGEENGDSEGAAPRTLSEEELLERLVREFGAKEVFEELDADPTPKD"
     gene            17132..17476
                     /locus_tag="VF752_11780"
     CDS             17132..17476
                     /locus_tag="VF752_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX8856266.1"
                     /translation="MPKQPNLNQMMQQVQAMQAEMAKAQEELKNETVEASAGGGMVTV
                     RVSGDLEVKDVRIDPDAVDPEDVEMMQDMVLAATNEALRSAQELAANRMNQAAGGLAG
                     PGGLGGLGLPGF"
     gene            17478..18083
                     /gene="recR"
                     /locus_tag="VF752_11785"
     CDS             17478..18083
                     /gene="recR"
                     /locus_tag="VF752_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX8856267.1"
                     /translation="MSSLSPPVNRLITELARLPGVGQRTAQRLAFYILRLPPDEALPL
                     ADAIREVKETVGLCEICFNLAVGPRCRICEDARRDASVLCVVEEPSDILPIERTNEFS
                     GRYHVLGGALSPIDGVDPEDIRIPELITRVGEGGVREVVVATNSTTTGEATALYIAEA
                     LREKQPEVAVTRLASGLPVGADLEYADEITLGRALRARREL"
     gene            complement(18100..19359)
                     /locus_tag="VF752_11790"
     CDS             complement(18100..19359)
                     /locus_tag="VF752_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8856268.1"
                     /translation="MARPRMPHGRPWIVAVGVIALIAAAIALPILLSGGADSGPSAML
                     PEASGQSGASPQSRTSFLSRIVPPPPQRTSGPPVPRSVLDLAKRLPLERKVAQLFLLG
                     FDGKGANSPIFRDMRRLDVGGIVLRTANYSNPAQLAQLTNAAASVARRHGHVPPWIMA
                     AQDGGDESAFADLPPDLAPAEEPSAQAAGADAAASAVTLRRLGLNAVLGPDLDVASAE
                     GTNVMGDLTFGANATDVAQFARATVSQYTRYGVLTAVKHFPGLGLASQSPDDGPAQVG
                     LSLDQLAQRDLVPFRAAFAAGAQAVLVGSGLYASDDFVTPASQSPTIIEQLLRGNLKF
                     RGVAIADDLEQAAISDSQDVSDAAIASILAGIDMVYISGPRGDQDAAYTAVLNAVRQG
                     RIPESRIDEAVLRVLQAKVHTGLIRSR"
     gene            19388..20680
                     /locus_tag="VF752_11795"
     CDS             19388..20680
                     /locus_tag="VF752_11795"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX8856269.1"
                     /translation="MPDAGSALAQTVVMKFGGTSVADAERIKRAAKRIVREKEAGNRV
                     VAVLSARGKTTDELVSMAYEVSERPHPREMDMLLSTGERISCALAAMVINDLGHEAIS
                     LTGSQAGIVTDTSHTKAKIMEVRADRIREALDDDKIVLVAGFQGVSTAYDVTTLGRGG
                     SDTTAVALAAELGADVCEIYTDVAGVFSADPRIVPDAKKLPVVSFEEMLEMAASGAKV
                     LQLRSVEYARNHNVRIHCRSSFDDGPGTFVYPEEETMERPLVTAVTHSTDEARITLTG
                     LPDQPGVAGRVLTVLADANVNVDMIIQNEPESDGHRADMSFTVPRADVPASLDALEPL
                     KGEFGIGVSSDDQMGKVSLVGAGMKSHPGVAAKAFRVLGDAGVNIEMISTSPIKISCV
                     VREDDVVRSVRDLAEAFELGDGGIRREDVQGDHRPVVS"
     gene            20700..>21177
                     /locus_tag="VF752_11800"
     CDS             20700..>21177
                     /locus_tag="VF752_11800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX8856270.1"
                     /translation="MRVAVVGATGAVGSTMLGVIRERAFPADEIVPFASERSAGRRID
                     HGSRTLTAEALSEESIRDFDIALFSAGSTVSEEWGPRFAEAGAVVVDNSSYWRMHDDV
                     PLVVAEVNPEAIDSHRGIVANPNCSTMQMVVALKPILDAAGIERVVVSTYQSVSGTG"
BASE COUNT         3441 a         7173 c         7421 g         3142 t
ORIGIN      
        1 ctcgccgtct cgccgcatca cctgagccgc accttccggg cgttgaccgg tcacacagtc
       61 tcgcggcaca ggatgcggct gagggcgcgt gccgcgatgg aacggatggc agcgggggac
      121 ggcgacctcg cacggattgc cgccgacctt ggcttcgccg accagagcca cctctgccga
      181 gtcatgcgcg ccgagaccgg gcgcacgccg tccgcactgc ggcgcgttct cgctgctgag
      241 taagccgccg actcgactca ggcagccagc gggccgggct cgctgtacgg cttgcagctg
      301 tggcaaatgc agcgggagcc gtggaccagg actagcgccg gcgcatcgcc atgagcccca
      361 actgctccgg acctctggga tctcgctgta cggcgctcgg tttcgtcgag cctgtggccc
      421 atgaacgagc gatctaggcg ctgggcttcg ctttggaaca tcgttcctcc cctaggacgc
      481 gcgtccgggt ttacacggaa tccgaggccg ccgtgaaccc gggcgaggcc ttctttccga
      541 ctggctgtca ttgtccgcgc gccgagcgcc ctgtcatcgc tgccagcccg agatgcgccg
      601 ccggaaaacc cgaggccggc catgggctgg ctggagggca cctgagagga tcgcgccatg
      661 accaagttcg aaggcttcgg gcccgacgta cggaagtggt tccagggtct cgaggcagac
      721 aacagcaggg aatacttcgt cgcgagccgc gacttcttcg aggagtcgat ccgaggccag
      781 atggaggccc tactcaccga gctcagcgag aagttcgggg gtgaggtgaa gatgttccgt
      841 cagaaccggg acatccgctt ctcgcccgac aagtctccgt ataagaccaa tacctacgga
      901 gtcgtttacg gctcgggcat ggccgcgcaa ggcctgtacg cgtcgatttc ggcgggcggt
      961 ctcgtcgccg gcagcggcta ccacgtgatg gcccgcgacc agctggatcg ctaccgcgag
     1021 gaggtcgcca acggcaagca tgggcaagag ttgacgaagc tcgtcgccaa ggcggagagg
     1081 gcgggcctgg aattgtgggg agagagcctc gccacggccc cacgcggcta tccgaaggac
     1141 cacgagcgga ttgagctgct gcggcgcaag aacctcacac tcggcgcgac gttgaaattc
     1201 gcgcgcggca tcagccgaag cgacggcctc cagttcgtcg cgaagacgtg gcgtgccgcg
     1261 gcgcccgtga ccggctggct cgacgagcat gtcggggcga gcacgatgcc cgtgcgccga
     1321 cctgcgcgca ggcgccgaac gtaacgccgc ctcgaagcgc tcaagcgctc gacatatccg
     1381 gacgttcctt cgagtgctgc cagagcgcct tgtccagtgt ccgcaggctc accccgtagc
     1441 gctgggcgag ctcgcggcag gcattcagat attcgagcca gaaggtcacc gggtactgcg
     1501 acctcggctt gacccccagc gactcgagcg cgcgcacgtc gaggatcggg tagtcgttcg
     1561 gaaacgcgac gtagagcaac gttgaagcgg tgggaacgcc aacgccttcc agcccgagca
     1621 acgcagccat acgcgcggcc tcctgctccg acgtggtgag cgcacgtccg gtcgcctcaa
     1681 tcacagctgc ctcggtatta ctcgcgacct tcgctctgct gcgcacggtc ttcccggcgc
     1741 agacctcgat gaactccgcg cgcgagtagt aaccccgggc tctggccgcc gcgccgagtg
     1801 ccagcaggcg cccgtcgtcg gcatgaggaa accgactcgc gagcgcctca atcccgctcg
     1861 ctggaaactg gagctcgaag gtcggcatct tcgaaagtct cgaagtcgtt cctcagcctt
     1921 ccctcacatc cgtgtccaca accacgcgta cgagatcacg tgcggctcaa atggctccgc
     1981 cgtcgcacac ggaggtatcg ctgagcgtga ctcacgatca cctgcttagg ggtcgcctcc
     2041 tagcatccga gtgacgatgg tggccgtgat cccgagcccc tcgagcaacg gctttcacct
     2101 cgggccgctg ctggtgcacg cctacgggct ggcctacgtc gtcgcggttc tggcggcggt
     2161 ggtactgacc acgcggcggt gggaagtgcg gggcggctcg cgcgaactcg tgtacgaggt
     2221 ggccctcttc ggcttcccgg ccggcctgat cggcggtcgc ctctacttcc tggccaccag
     2281 ctggaacgag gtcccggacc actggtgggg gccgttcgcg atctggaaag gcggcctggg
     2341 gatctggggc gggatcgccc tgggcacgct cgctggcgtg tgggtgctgc accgtcgccg
     2401 cgctgacatc cctcgcttct tggacgccgc cgcccccggc ctgctcgtcg cgcaggcgat
     2461 cgggcgggtc ggcaactact tcaatcaaga gctgttcggc ggacccacca acctgccctg
     2521 gggcatccac atcgacccgg cgaaccgccc tgccggctac gcgcactaca ccaccttcca
     2581 gccgaccttc ctctacgagc tgatctggaa cctcgcgctc gctgccgcgc tgatttggct
     2641 cggccggcac cgccagatcc gggcgccggg cctattcgcg ctctatgtgg cgggctactc
     2701 gggcttccgt atcttcgaag agcttctacg ggtcgacccc gcccaccaca tcctcggact
     2761 gcggctcaac ttcttcgtgg ctgtactgct gttcgccgcc ggtctcgcat ggttcgctcg
     2821 catccagcgt ggaggcgcta cgcgacgact ggtctcgcgc ggtggccccg ccgcgcttgg
     2881 ggtcgcgtgg ctggcttgcg ttctggctgg ctgcggtcaa gcccaccgac ccgagaccgc
     2941 gagcagcgca ccggccccgc tcctccgggc aagtacagcc ggccgcgagc caaccacccc
     3001 gcgacaactt ctggtggtcg atctgtcaca gatgcgtact gctatgcgcc gagctgatcc
     3061 ggtccacccg cctggcccgt gaactccagg tcccagctgt cgcgatcgcg tttgcgggtc
     3121 agtgggctcg cctgcgaacg aggtgccggg cgagtccgag cacgcggggc agggcatgtc
     3181 gcaggtctgg cgcgcaactc gcaggaccca gacgccctcg cagtcggcgc gcgcgggctg
     3241 cgccgctacc tggaatcgct gcgcgcttag tgacgggcgc cggcaacact ggctgagaca
     3301 tcaccagcag acgccgcacg ctcgtcgaac tgtcgctttg gatgacgagg agcagccgcg
     3361 cccggcgcgt gcggcgctgc tgctctgcat ggaaggcgca agctttccgc ccgcgagata
     3421 ctgcacgcgc gatgtcggag aagaacgtcg aaacagttcg ccgggtctac gaaggggtga
     3481 acgcccgctt ggaggtaccc cgcgagctgt tcgacccggc ctatcagttc gacaacacgg
     3541 agctctggcc ggatatcggc gaagtcctgg gatttgaagc ggcacaggaa gctatgcgcg
     3601 agtactggga aacgttcgag gcttaccacg tcgagatcga cgaggtgatc tacgccgacg
     3661 aggggcgggt tgtcgatgtc gtccgcgatg gcgggcggat gagagggacc gacgccgagg
     3721 tatggaaccg cttcttccac gtctggaccc ttcgtgatgg gaggatcgtt cgcctctcga
     3781 tacacaccga ccggaaccga tccctcgaag ccgctgggct gtcggagtag cgcgcagaag
     3841 cacttgcctt ggacgacatc cggcagtgcc gcctcgcatg gaacgcaata gcgggagcgt
     3901 gactgctgcc ttcagcggat gcaggagcgc gctcactgcc cggcttcgct gtcgtcgggc
     3961 gcggcgtagg ctccgaaatt cgtgatggca gcgaccgggg aacagttcct gcgcgagggc
     4021 ggcgaggcga tcgccgcggc ggactgggag cgggcacggg cctgctttga agaggcgcgc
     4081 gagctcgacg gcgctcccga ggcgctcgac gggctcagcg aggtcgcaaa cttcgagggc
     4141 gactacgagc gggcgattga gctcaaggaa gcggcgctcg ccgcctaccg cgcacgcgac
     4201 cagcgcgtcg aggcctcgca cgtggcgcgc tggctggcat tcatgcacgc gacctatcac
     4261 ggcaactacg ccgtcgcgag cggctggatg gcgcgggccg aaagcctgct cgatggcgtc
     4321 gaggagtgcg cggcgcacgg ctggctgatc ctcgaccgcg cgcccttttc ccgcagtccc
     4381 caggagcggc agcaggtcgc ggcttcggcg cttgccatcg ctcggcgctt cggggacgct
     4441 gagcttgagt tcgaggcgat ggcgctcctc ggcgagtccc gcgtcgcgtt ggggcggatc
     4501 gacgagggca tgaagctcct cgacgaggcg atggccgcgc tcgccggtgg cgagatcgcc
     4561 gaccacaagg cgatcggcga gatctactgc cggctcctca gcgcctgcga gcaggcgacc
     4621 gacgtgcggc gggcggagga ctggatgtcc gcgatcgacc gccatgtcgt ttggacggat
     4681 ttcgtgcggc caacgtgccg cacgcactac ggcggaatcc tgatggcgct ggggcgctgg
     4741 caggaggcgg aggccgagct gctggcggcg atcaagacgt tcgagcgggg ctaccgcggc
     4801 gacggggcgt tcccgctagt ccggctggcc gagttgcgcg tccggcaggg ccggttcgag
     4861 gaggcggagc ggctcctcga aaacagggag tggcatccac tggcgcggcg ggcggcggcg
     4921 gcgatcgcgc tcggccgccg cgagctgccg ctcgccgagg agctcacgcg gctgtgcttc
     4981 gaaggtgcag ccgacgatga cccggcctgc gggccggccc tcgtgctcct cgtcgccgtc
     5041 cagctcgaga ggggcgatga agagggcgcc cgatcaacgc tcgaccggct cgcggggctt
     5101 gccgcgagct gcgggagcca gagcctcgaa gcctcggttg aactctcggc cggacggttg
     5161 gcgatccttc ggggcgacga cgcggcgatc ggccacctaa agcgggcggt cgagcggttc
     5221 gccgggctcg agctgccgtt cgagaccgcc cgggcccgcc tcgagctggc gcgcgccctt
     5281 gcgccaacca ggcccgatgc cgctgcgtat gaggcacgcc ttgccctcga tgcgttcgaa
     5341 cgcctcgggg ccgcgcacga ttgcgacgct gcggccgagc tgctgcgaac cctgggcgcc
     5401 tcgggacgag cgtggcccaa ggggcgcgga ccgttgacta agcgcgagac cgaagtgctc
     5461 gcactgctcg tcgaaggcct tgccaacgcc gagatctctg agcggctgtt catcagcgtg
     5521 cggacggccg agcaccatgt ggcgagcatc ctcgcgaagc tcgatctgcg cagccgcgcc
     5581 gaggccgccg cctacggggt ccgccagggc gcggcaaagg accagtaggg aaatgggtag
     5641 tcctgccgat gctcgatcgg caggtcgggg agatccttga ccagggtcgc gggctgcgac
     5701 ccggacaagc gatcgaagga gggtcagatg agtgacagcg agcagaacaa ggcggccgtg
     5761 cgagactgct tcgcagaggc gtcgcgcggg aactttggcg cgctcgactc catcctgagc
     5821 cccgattacg tcctccaccc agagggggtt cgcggctccg agggcctcgc cgagatggtc
     5881 cagggctacc gcgaggcgat cggcgggctg accgtgacga tcgatcagca gttcaccgag
     5941 ggcgattacg tcgcgacgcg gttcacgatc cgcggtcgtc acgagggcga gctgatgggc
     6001 acggagccga ccggccgcga cgtcgagttc gccggcctca cggtgagccg ctgccgcgac
     6061 ggcaggatcg aggaggagtg ggagcttgtc gacactgtga gcctcctgaa gcaggtcggc
     6121 gcccttcctg aggtggctca ggcctgacgt ttgaccaggc gatgccgctc ggtgccgtcg
     6181 gcggctggcc gcgaaggagg cgccgcccgt tgggtcgcgc ctccttcgcc cgcaagggag
     6241 gcgcctccac cgagctgcca cgcctagcgc tgtggatgaa cagcgggagc gcaggttgag
     6301 accgcttctc gcagcgcccc agcgcaccgt tccgaaacgg cgaaaccccc gcggatgcag
     6361 gggttccgga cgatgggcac ggctggtttc gaaccagcga cgtctcgcgt gtgaagcgag
     6421 cgcgtaagcc gcggacatcg aattgatccc cctgcaaatc gccgctatct gcgtttccga
     6481 gctgcgaagc cggttgggct acgtgcggtt tcgtggggct gcgtttggga caacgattga
     6541 gacacccccg agcccggatc cgcccgtcac gccgctaacg tcgagcgccg aggccggacg
     6601 tcgtccacga gctcggcgcc acggagtccg cgctcaacaa gctcggggcg cgcaacatct
     6661 cggccgacga ggccggacag ctcgcgcgga agcagcacat cacggtgcgt aaccgtgtgg
     6721 atcggagggc cgagatgggc ggctgattat cggcaggacc gacgggtggg cgagcgttga
     6781 cgctcgtgat cgagcaaacc gtcgagccga cgacgcggcc ggcgaccaac gtcgcgcctg
     6841 ccgcgcccag cgcttgcccg gtgcggaaaa agggggactc agcgcaacgc ctcgaggggg
     6901 gtggcgggct cgggtgggtg gacgcgccgc tcgcgacgtg ccacgccgct ggcgaccgcc
     6961 gcgcccgcga cggcgtcggc caccgattcg gcgacgtggc ggttgaacac gctgggaatg
     7021 atgtagtcgg cgtgcagctc ctgctcggga atgacgtgag cgatcgcgtg tgcggcggcg
     7081 agctccatct cctcgttgat cgtgcgtgcg cgcacctcga gcgcgccctt gaagactccg
     7141 gggaatgcga gcacgttgtt gatctggttg gggtagtcgg agcgcccggt ggccatgacg
     7201 gcgacgtcgt cgcgtacctc ctccgggtgg acctccggca cgggattcgc catcgcgaac
     7261 acgatcgcgt ccggcgccat tgccctcacc ccagctggcg acaccgcgcc gggccctgac
     7321 aggcccagca gcacgtgggc gccggccagc acctcatccg ccttgccccg cagcccgcgt
     7381 ttgttcgtcc gttccgcgag tgaggctcgc tccgcgtcga gatcttccgc gccggcgtag
     7441 agagcgccac gccgattgca cacgaccacg tcgccgacgc cctgcgcgag caggatctcc
     7501 gtgcacgcca cgccggcggc gcccgcgccg acgaccacca ccgtcacgtc ttcgggccgc
     7561 ttaccgacga ctcggagtgc gttgagcagg gcggcaagga cgacgatcgc agtgccgtgc
     7621 tggtcgtcgt gaaagaccgg aatgtcgagc tcagcgcgca gccggcgctc gatctcgaag
     7681 cagcgcggcg cggcgatgtc ctcgaggttg atgccgccga acgtcggcgc caccgctctc
     7741 acgaacgcga cgatctcgtc gacctcttcg aggtcgatac acagcgggaa cgcgtcgacg
     7801 cccgcgaact ccttgaacag cattgccttg ccttccatca caggcatagc cgccgccgga
     7861 ccgatgtcgc caaggcccaa gactgcgctc ccgtccgaga cgaccgcgac ggtgttgctc
     7921 ttgatcgtca gcgcccacgc cttgcccgga tcctcatgga tggccgtcga gacccgtgcg
     7981 accccagggg tgtacgccat cgagaggtcg tcgcgggtct tgagcggcag cttggcgttg
     8041 acctcgatct tgccaccctt gtgcatcaga aaggtccggt cggagacgct gtcgacccgc
     8101 acgccttcga gagcacgcac agcgcgcacg accgcctcgg cgtgcccagc gtccgcgcac
     8161 gcgacggtca catcgcggac gacctcttgc cgttccacgc gcaccagatc aatcgcgccc
     8221 aacaacgcct ctgactcggc gattgcctct gcgacgtggg cgaacgcgcc gccctgctgt
     8281 ggcatgtgga ggcgcattgt gaagctgtac gaggccgagg gctgaagccg cagggacggc
     8341 accttcgggg attgatcgga catcgggcct ccttcgccgc tttccgtcgg ggcacggccg
     8401 ccggccgcct ccccctggtg accctgcgag gcggtcacgt gccgcaccgg cctgccggtg
     8461 atgcgctcca cgcgacgggg caggtcgagc ttgagccacg gtgagatccg cgcgggcagc
     8521 gtggagacaa tgacctcgtc gaaccgctgg ggatcccaga gctgctgcac tgcgagggcg
     8581 gggtcgggat ctccgagcac gccttcgttc gcttggcgac tacgagcacc tcggccttag
     8641 cggtcatcac gacctcctcg cgccactaat gcggatagga ccatatcgcg cgccgcgcga
     8701 ggcgcatcag tggctgcctc cggaaagaca cacgcgcata cttcacgggg tgtacgcgtg
     8761 cgcttactgt ggtcgagctg cgccgcgctt caatgatccc gccatcctcg agtggacgaa
     8821 cggcgaggcg cggatcatgt ccgatgatcc cgagccgctg ccggagagcc tcgtttgtcc
     8881 aggctgtcgg aacgcggagc gcgagcccga cgaaggggca ggggggctag gcgccaggag
     8941 agtccggctg ttcgttgtct aggaggttga aaccatggga gtagaagatc aggcaccctc
     9001 cacggcagag gtcggcgagg cggggctgtc tgaggcgcag atagcggttc attggcgcga
     9061 ggaggagtac tacccgccgc cggagacgtt tgtcgtgcag gcgaacgcga acgacccgtc
     9121 gatcctggag cggttcggcg aggagcattt cccggactgt ttcgtcgagt acgcggagat
     9181 gctcgattgg gacacgcggt gggagaccgt tctagacacg agcaaccctc cgttctggaa
     9241 gtggttcgtc ggcgggcgcc tcaacgcgag cgtcaactgc ctggaccggc acctcgaatc
     9301 gagaggcagc aggaacgcgc tgatttgggt gcctgagccc gaggacgagc agacccagta
     9361 catcacgtac gaggatctgc gccggcgtgt gaacgagttc gcggcgcttc taaggggctt
     9421 cgccggggtg gagacggggg accgggtcac gttccatctg ccgatggtgc ccgaactgcc
     9481 cgtgtcgatg ctggcgtgcg cccggctcgg tgtgatccat tcggaggtct tcggcgggtt
     9541 cgccggtacg gcgtgcgggc accggatggc cgactcgcag agcaagatcc tcgtgacgat
     9601 ggacggctac taccgcaacg gcgagctgat cgaccacaag tcgaaagcgg acgaggcgat
     9661 cgccgccgcc cgcgaggaag gagtcgaggt cgagaaggta ctcgtgtggc ggcgccaccc
     9721 ggacgcgtat gcgtccaaca gcccgatggt cgatgggcgc gacttcttca tcgacgagct
     9781 actgaaggac tacgagggca acaccgtgga gccggcgtcg atgccggcgg aggcgccgct
     9841 gttcttgatg tacacgagcg gcaccactgg acggcccaag ggctgccagc actccaccgg
     9901 cggatacctc gcctacgtga ccggcacctc gaagtactac caagacatcc atcccgatga
     9961 tgtctactgg tgcttcgcgg acatcggctg gatcaccggc cactcgtaca tcgtctatgg
    10021 gcctctctcg ctcggcacca ccagcgtgat gtacgagggc gtcccgacct acccggacgc
    10081 ggggcgacca tggcggatcg ccgagaagct cggggtgaag atcttccaca ccgcgccgac
    10141 gacgatccgg atgctgcgca agcttggacc ggacgagccg gccaagtacg actaccactt
    10201 caagacgatg acaacggtcg gtgagccgat cgagccggac gtctggcgct ggtactacca
    10261 ggtcgtgggc aagggcgagg cggcgatcac cgacacctgg tggcagaccg aaaacggggg
    10321 cttcctcggc tcgacgctac ccgggctgca gcggatgaag ccgggcagct gcggcccggg
    10381 cgtgctcggg atctacccgg tgatctacga cgaggagggc aacgaggtcc cgaagggatc
    10441 gggcaaggcg ggcaacatct gcatccgcaa cccgtggccc gggatcttcc aaacgatctg
    10501 gggccagccc gagcggttcg tcgataccta ttacacgaag tactgcaagg accggggcag
    10561 cacggactgg cgtgactggc cgtacttcgc cggcgacggt gcggtgcagg cggccgacgg
    10621 ctacttccgc atcctcggtc gggtcgatga cgtgatcaac gtcgcgggcc accgactcgg
    10681 caccaaggag ctggaatccg cagcgatcat ggtcgacgag gtcgccgagg cagcggccgt
    10741 gccggtaatc gacgaggtgc gcggcaagat cgtcgagatg tacgtggcgc tcaagcccgg
    10801 cttccagccc agtgacgaga tcgcgcagaa ggtctcgcag acgatcgtca aggagatcgg
    10861 tgcgatcgcc cgcccgaaga acgtgtggat cgtggccgac atgcccaaga cccgttcggg
    10921 caagatcatg cgtcgcgtca tcgccgcggt ctcaaacttc gccgatgtcg gtgacgtgac
    10981 tacgctcgcg aacccggaaa tcgtccagca aatccgccac cacgtgcaga gcgagaagct
    11041 cgcccacggc gaggtgccgc gcgagctctc agagaaggag agacaagaga tcgcagcgta
    11101 cggcgcggaa taaccgggac gccgtgccta ctcgggcatc cacccggcgt tgtcggcgaa
    11161 ctcaccgagc gcggcagcgg ctctcgcata acgaccaaag tcggcgtcgc cgcgttcgag
    11221 ggcgtcccaa cagatcacgt cggtcaggtc catcgcctcg tgcgccagct tcgggagcgt
    11281 ccgaagcggg caacgctcga cctcccgtcg tagctcgcag acagcatcag caagtcgcgc
    11341 gcccgtaggt ggttgcagct cattgagcaa cgccgacaac tgcccgatca ggtactgctg
    11401 ggtcaccgcg agttccgagg ctgggcgggc tcagcctagc tcgccgccgc acgtcaggca
    11461 atggccggag tcgtggacgg gaccgcgacg aagcgcgccc cgtccttccg gctcagagga
    11521 cacagagccc ctacgacgcg cagcgcgtgc cggtctcgcc ttcgacgatg ggcgcggctg
    11581 gtttcgaacc agcgacctct cgcgtgtgaa gcgagcgctc tcccactgag ctacgcgccc
    11641 tgggatgggg cagtttaggc gcacccgggg gagttgtcct cgcggcaagc ccgttttacg
    11701 atcgggccat ggccgccgat cgcggggcga tcagcgtcaa cggtgcggac atcgcctgga
    11761 tggccgaggg cgcgggcccc gacgtcgtcc tgatccactc cgcgatcgcc gacatgcgga
    11821 tgtgggacga gctggtggga cggctccgtg accgccatcg cgtcatccgg tacgacatgc
    11881 gcggctgggg cgagagcccg cggccgccag ggcgctactc gcacatcgag gatctcgccg
    11941 cgctgctcac cgagctcggc gacggcccgg ccacactcgt cggctgctcg ctcggctcac
    12001 gcgtcgccgt cgaattcgcg ctcgagcacc cggacgcggc gagagcgctc ctactgctct
    12061 cgccgttcct gcgcggtcat aaatggacgc cggagctcgg ccagttctgg gccgagcggc
    12121 ggcaggacct cgacaacggc gacattgacg ccgccgtgcg gctcaacgtc gagcagtggg
    12181 caggtggcct ccctgaggaa cagcggcaac tgatcgcgga catgcagcgg cgcgccttcg
    12241 agctggagct ggccgtcgac gcccagcagg acggcttcga gccgccggcc gcccagcgac
    12301 tcggtgagct caggcttccg acgctggtcg ccgtcggcga gcgcgacttc agcgacttcc
    12361 acgccgttgc cgaccggctg gcgcgcggca tcgcccactc gcgcagcgcg atggtgcccg
    12421 gcgcgggcca cctgctgccg atggaacgcc cgcgcgagac cgccgggctg ctcgaggagc
    12481 tcgaacgagc gcctacctcg gcgatcgagc gcggacccca gatgtagaag agggcatgac
    12541 ccgttacgac gcctatctct tcgttcatgt agccgcgacc atcatctggg tcggctccgg
    12601 ttctcttcta acagtgctcg cgtaccgcac cgagcgcgcc aacgacgagc agaggatccg
    12661 caacctcctc gacgactcga acgccctagg cacaaggctc ttcatcccgg ccgccctcac
    12721 ggtcgtggcg ttcgggatcc tgatggtcgt cgatggcccg tggacgttca gctacctctg
    12781 gatcgtgctc ggcctggtcg gctacgccgc gacgtccgta accggcgcgt tcgtgatcaa
    12841 gccctggagc gagcggatcg cggagctgat caagcgggag ggctcggtca cgccggccgc
    12901 gatggcggag atccgccggc tgctgtggtt cggccgcatc gacctcctga cgctgttcct
    12961 cgtgatcttc gacatggcgg tgaagccgag cggcgacgac accgcaatcc tggtcgggat
    13021 ggcgctcgtc ttcctcgccg gcgcggcgtt cgtggcctcg cggattcgcg gggtcgagga
    13081 ggcgccggaa ccgcaggtcg caacgggctg acggcgctag gctttgcagc ggaccgtgct
    13141 aggcggggag gtagcggtgc cctgcactcg caatccgctc tagcgaggct gaatccccgt
    13201 ccgagggatt gaagcttcag ggccagtacg ccggccacaa ggcgtcgatg gccaggtccc
    13261 gtgcgatgga cggctgtgaa ccaggtcagg tccggaagga agcagcctta agcggtaccg
    13321 cccatgcgcc acggggaagc ctgaccggag tcctggaccg gcgggaacgc ctgaagctcg
    13381 ttctcgaaga tgggtgcacg gtccgttctt cattccgggt cgcgacagga gtggagcgtg
    13441 ctccagacgc tgaggattcc gccgctcaca gcctgcgtgc tcgctgccgc cgtgctgacc
    13501 ggatgcggtt cgagcggtgg cggggcccac aagccgctgc caccgcccgg gaacatcaag
    13561 gtcacgagtt ccgccttcgc tcagggaggc acgatcccca agcggtacac gtgcgacggc
    13621 gcgggtttat cgccgccgct cgcctggagg cgcctgcccg acgcgacccg ctcgctcgta
    13681 ctggtcgtgc tcgatcccga cgcgcccggt ccgaccttct tccactgggt gatctacgac
    13741 ttctcgccga atcagcggga agtggcggcc ggcgagagcc cgcgcggcgc agcggcaggt
    13801 gagaacagcg ccggcaaagc gggctggacg cccccgtgtc cgcccaaggg caactcgccg
    13861 cacaggtacg tgttccggct gttcgccgat agcaagccca ctgacctcgc cgccggcgcc
    13921 gctccctcag acgtgctggc ggcggccggg acgcattcga tcgcacaggg aactctgacc
    13981 ggccgctatg gccgctgaca ggccggtccg gcgtagagca cccacgagcg gttccgcgct
    14041 gcttcgcgcc aaccgggcgg cacgcgcagc tggatcacgg tcggctcgtg cgggtcgagg
    14101 atcgagagct tctcgacctc ggcgctggcc gggaagaaca tcgtgccgcg gctcgctgcc
    14161 gcacccggtt gcctagtgac gatcgacgcg ggccggatgt cgtcccagta ggcgatcagc
    14221 ggaaccggcc ggtggttcgg cacgtaggtg gggcgacagc cgcgcaccag cgacttcacc
    14281 ggcgcgcgcg acgccaggcc gtgcaggtcg gcctgaatgc ggtcgcggtt gccgatgtcg
    14341 gtacggagcg acttgagccg gtccacctgc tggacggcga agaagaggac caccagcgcg
    14401 acgatcgccg agccgccgat gctccacgcc cgccgccagg gatgcgaggg ctcgagcgcg
    14461 gtccaaccga gcgccgcgac cgcggcgaac agcgcgagca tcgtgccgag caggaacagg
    14521 tagcgcccga gcagcgacag cccggcgatc gcgaacacga tccaggcgac gccgttcagc
    14581 accgcgatcg aggttgggac cgccatccgt ctgccgaaaa cgcgccagcc gaccacgaaa
    14641 ccgatcaccg aagcgattag ctcgggaagg cgcaggatct cgccgagccg gcgcgcgccg
    14701 accggcacgt ggcgcagccc ggtctggcgg ccgagctgct gcgcgaggtc gctcgttccg
    14761 ttcagcgacc agaggaagtc gccggtgatc gcgagatcgc tgaagcccca gatcagcggc
    14821 gccgccgcgg cgagcagcgc gagccggatc cggccagccc agtcgcgggc ggggaacatc
    14881 cacagccagt aggcggcgat gaacagccac gcctccggcc gcagcagccc ggcgaggccg
    14941 agcaggacga gcacgggcca gccgcgtcgc ggccttcgcg cctcgagcac gcccgcccag
    15001 acgatgaacg ccattgcggc gaagtccacg tagccgcgga tcgcgaagtt gagatagggg
    15061 acgcgcgtgg cgacgagcag cgccgcgagc agcccgaccg gccaggagaa cagctcctca
    15121 cccaagcgga agatgcccac cacgagccag ccgaacgcga acaggatcac gccgagcatg
    15181 gcgttctcgg cgccgtcgcc gagcggcgag agcacgagcc cgacgagctc cgcgagcggg
    15241 tgcggcgtag gtgcgaccgg cacctcgtac tgcggcttca gcccgtgcgc cagatcgtgg
    15301 ccccagagca gcgcgtagaa ggtgtcgtag ttcagaaagg cgtggccgaa ccacgcccag
    15361 acgaccgcgg cgacgactgc ggcggcgact gcatcgacca ggaggccgcc accccggggg
    15421 cggcgaggtc ccaggctagg atgcatccgc cgatgctttc cctctaccgc cgccaccgcc
    15481 cacgcacctt cgacgacgtg gtcgggcagg agcacatcgt gcgcacgctg cgcaacgcta
    15541 tcgagctgga caaggtgcac cacgcctacc tcttcgtggg gtcgcgcggc accggcaaga
    15601 ccagcatggc gaagcttctc gcctccgccc tcaacgcgcc gggcggcccg aaggccgact
    15661 tcgatcccga cgacccgtcc gcggccgcca tccgcaacgg cacgtcgctc gacgtggtgg
    15721 agatggacgc cgcctcgaac aactcggtcg acgacatccg cgagctgcgc gagaacgtgg
    15781 cgctcgcccc gatgggcggc agccgccggg tttacatcct cgacgaggcg cacatgctct
    15841 cggcctcggc gtggaacgcg ttcctgaaga cgctcgagga gccgccgccg cacgtggtgt
    15901 tcgtgctcgc caccacggag gcacacaagg tgccgcccac gatcgtcgac cgttgccacc
    15961 gcttcgactt ccaccgcccg tcgctcgagc agatcgtcgg cgtactgcgc aaggtctccg
    16021 cggaggaggg catcgacgtg cccgaccagg cgctcgtgat gatcgcccgc tccgccaccg
    16081 gcagcttccg cgacgcgctc ggcacgctcg agcagctcgt cacctacggc ggcaagaacg
    16141 tccggctcga ggacgtgctc gaggtgctcg gcgtggccga cgccgagctg gtgctcgagg
    16201 ccgcgcaggc gatcgccgac cacgacgcgc gcgaggcgct gctcgcggtc gagcggctgt
    16261 cggcctcggg tcgcgacctc gtgcagttca tgcgcgacct cgcggcgcac ctgcgccacc
    16321 tcttcgtcgt gcagacgctc ggtgaggtgc ccgagtcgtt cgcggtcacc gcgcagcaca
    16381 cggaccgcct ccaggcgcag gccgaggcgc tcgcgcaggg agagatcctg cgcgcgatcg
    16441 acttgctggc ggccgcgctc tcggcggtga aggacggctc ggagccacgc atccagctcg
    16501 agctcgcgtt gctgaaggcg gcgcaccccg agtcggacgt gacgctcgac gcgctcaccc
    16561 gtcgagtcga gcggatcgag aagtcgctcg gcgggggacc agcgcccgcg ccggctgcgc
    16621 ccgaggccgc gcgtccaccg gaggtcgccc ccgagccggt gcgagcggcg caaccggacc
    16681 cggagcccga accgcagccg gaccccgagc cggagccgga gccggccgcc gagccggcga
    16741 gtgacctggg cctcgaccga gtgcgcgagc tgtggcccgc cgccgtcgac gccgtgcgtg
    16801 tggagaacgc gatggtggga gcgctgctcg ccgaggcgcg cccgaccgcg ctcgagggtg
    16861 agcggctcac cgtcaccttc ccgggcgact cgacgttctc gaagaagaag gcggagtcga
    16921 accgcgtgct gcttcacgac gcgctgcacg gcctgacggg ccggtcgttg accctcacct
    16981 acgagctcgg ggaggagaat ggcgacagcg aaggcgccgc cccgcgcacg ctgagcgagg
    17041 aggagctgct cgagcgcctg gtgcgggagt tcggcgctaa ggaagtgttc gaggagctcg
    17101 acgccgaccc gaccccgaag gactgaccgt catgccgaag cagcccaacc tcaaccagat
    17161 gatgcagcag gttcaggcca tgcaggccga gatggccaag gctcaggagg agctgaagaa
    17221 cgagaccgtc gaggcgagcg ccggcggcgg gatggtcacg gtgcgggtct cgggcgacct
    17281 cgaggtgaag gacgtccgga tcgatccaga cgcggtcgat cccgaggacg tcgagatgat
    17341 gcaggacatg gtgctcgccg ctaccaacga ggcgctgcgc tccgcgcagg agctcgccgc
    17401 caaccgcatg aaccaggccg ccggcgggct cgcgggaccc ggcgggctcg gcgggctggg
    17461 cctcccgggc ttctgagttg agctccctct ccccgcctgt caaccgcctg atcaccgagc
    17521 tggcccggct ccccggcgtc ggtcagcgca ccgcgcagcg gctcgcgttc tacatcctgc
    17581 ggctcccgcc tgatgaggcg ctgccgctcg ccgacgcgat ccgcgaggtg aaggagacgg
    17641 tcggcctgtg cgagatctgc ttcaacctcg cggtcggccc gcgctgtcgc atctgcgagg
    17701 acgcgcgccg cgacgcctcg gtcctgtgcg tggtggagga gccgagcgac atcctgccga
    17761 tcgagcgcac caacgagttc agcgggcgct accacgtgct cggcggcgcg ctgtctccga
    17821 tcgacggcgt ggacccggag gacatccgca ttccggagct gatcacgcgc gtgggcgagg
    17881 gaggcgtgcg cgaggtggtc gtcgccacga actcgaccac gaccggcgag gcgaccgcgc
    17941 tctacatcgc cgaggcgctg cgcgagaagc agcccgaggt ggcggtcacg cgccttgcga
    18001 gcggcctgcc ggtcggcgcc gacctcgagt acgcggacga gatcacgctc gggcgcgcgc
    18061 tgcgcgcccg gcgcgagctg tgatcgccct cagcggcgct cagcgcgagc ggatcagtcc
    18121 cgtgtggacc ttggcctgta gcacccgcag caccgcttcg tcgatgcgcg actccggaat
    18181 gcgcccctgc cgcacggcgt tgagcaccgc cgtgtacgcg gcgtcctggt cgcctcgcgg
    18241 tccggagatg tagaccatat cgatgccggc gaggatcgac gcgattgccg cgtcgctcac
    18301 atcctggctg tcgctgatcg cggcctgctc gaggtcgtcg gcgatcgcaa cgccgcggaa
    18361 cttgaggttg ccgcggagta gctgctcgat gatcgtcggc gactgggagg cgggcgtgac
    18421 gaagtcgtcg ctcgcgtaga gaccagagcc gacgagcacc gcctgtgcgc ccgccgcgaa
    18481 cgcggcgcgg aagggcacga ggtcacgctg cgcgagctga tcgagcgaca ggcccacctg
    18541 ggcggggccg tcgtccggcg actgtgacgc caagccgagc ccggggaagt gcttcacggc
    18601 ggtgagcacg ccgtagcgcg tgtactgcga caccgtcgcg cgcgcgaact gcgccacgtc
    18661 ggtcgcgttg gcgccgaagg tgaggtcgcc catcacgttc gtgccctcgg ctgaagccac
    18721 gtcgagatca gggccgagca cggcgttcag cccgagccgc cgcagcgtga cggccgaggc
    18781 ggccgcgtcc gcccccgcgg cctgcgccga gggctcctcg gccggcgcga ggtcgggcgg
    18841 caggtcggcg aacgcgctct cgtcgccgcc gtcctgcgcg gccatgatcc acggaggtac
    18901 gtggccgtga cggcgcgcga cgctcgccgc ggcgttcgtg agctgagcga gctgggccgg
    18961 gttcgagtag ttggcggtgc gcagcacgat cccgccgacg tcgagccggc gcatgtcgcg
    19021 gaagatcggc gagttcgcgc ccttgccgtc gaacccgagc aggaacagct gcgccacctt
    19081 gcgctcgagc ggcagccgct tggcgagatc gagcacgctg cgaggaacgg gcggtccgct
    19141 cgtccgctgg ggcggcggtg ggacgatgcg gctgaggaac gaggtgcgcg actgcggcga
    19201 cgcgccgctc tgcccgctgg cctcgggcag catggccgag ggaccggagt cggcgccgcc
    19261 cgagagcagg atcgggagcg cgatggctgc ggcgatcagt gcgatcaccc ccactgccac
    19321 gatccacggg cgtccatgcg gcattctggg tcgggccact gcggggggaa agctatcttt
    19381 ctgcgccatg ccggacgccg gttcagcatt ggcccagacg gtcgtcatga agttcggggg
    19441 cacctccgtt gccgacgccg agcgcatcaa gcgcgccgcc aagcgcatcg tgcgcgagaa
    19501 ggaggcgggc aatcgcgtcg tggccgtgct gtcggcacgc ggcaagacga ccgacgagct
    19561 cgtctcgatg gcctacgagg tgtctgagcg cccgcaccct cgcgagatgg acatgctgct
    19621 ctccaccggc gagcgaatct cgtgcgcgct tgccgcgatg gtgatcaatg acctcggcca
    19681 cgaggcgatc tcgctgaccg gctcccaggc aggcatcgtc acggacacct cgcatacgaa
    19741 ggcgaagatc atggaagtgc gcgccgaccg catccgcgag gcgctcgacg acgacaagat
    19801 cgtgctcgtc gccggcttcc agggcgtgtc aaccgcatac gacgtgacga cgctcggccg
    19861 cggcggctcc gataccacgg ccgtcgcgct ggcggccgag ctcggcgccg acgtgtgcga
    19921 gatctacacc gacgtcgccg gcgtgttcag cgcggacccg cgaatcgtcc cggacgcgaa
    19981 gaagttgccg gtcgtctcgt tcgaggagat gctcgagatg gccgcctccg gggccaaggt
    20041 cctccagctc cgctcagtgg agtacgcgcg caaccacaac gtgcgcatcc actgcagatc
    20101 gagcttcgac gacggacccg gtaccttcgt ctatcccgaa gaggaaacca tggaacgccc
    20161 gctagtcacc gccgtcacgc actcgaccga cgaagcccgc atcacgctta cgggcctgcc
    20221 ggaccagccg ggcgtcgccg gccgcgtgct gacggtgctg gccgacgcca acgtgaacgt
    20281 ggacatgatc atccagaacg agcccgagag cgacggccat cgagccgaca tgtctttcac
    20341 cgtcccgcgc gccgacgtgc cggcctccct cgatgcgctc gagccgctca agggcgagtt
    20401 cggcatcggc gtctcgagcg acgaccagat ggggaaggtc tcgctcgtcg gcgctggaat
    20461 gaagagccac ccgggcgtgg ccgcgaaggc gttccgcgtg ctcggcgacg ccggcgtgaa
    20521 catcgagatg atctcgacgt ccccgatcaa gatctcctgc gttgtgcgcg aggacgacgt
    20581 cgtgcgctcg gtgcgcgacc tcgcggaggc cttcgagctc ggcgacggtg ggatcaggcg
    20641 agaggacgtg cagggcgatc accgtcccgt ggtctcctga cggacgagag gagcggtcga
    20701 tgcgtgtagc agtggtggga gcgaccggag ccgtaggctc gacgatgctg ggcgtgattc
    20761 gcgagcgcgc gttcccagcc gacgagatcg tgccgttcgc ctcggagcgc tccgccggcc
    20821 gcaggatcga ccacggcagc cggacgctca ccgccgaggc gctgtcggag gagtcgatcc
    20881 gcgacttcga catcgccctc ttctcggcgg gctcgaccgt cagtgaggag tgggggccgc
    20941 gcttcgcgga ggccggcgcg gtggtggtgg acaactcgtc ctactggcgc atgcacgacg
    21001 acgtgccgct cgtcgtcgcc gaggtgaacc ccgaggcgat cgactcccat cgcggcatcg
    21061 tcgccaaccc gaactgctcg acgatgcaga tggtcgtggc gctgaagccc atcctcgacg
    21121 ctgccggaat cgagcgtgtc gtcgtctcca cctaccagtc ggtgtcgggc accggcc
//
LOCUS       DASVXS010000210         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_482477, whole genome shotgun sequence.
ACCESSION   DASVXS010000210 DASVXS010000000
VERSION     DASVXS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482477"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1162)
                     /gene="trpD"
                     /locus_tag="VF752_11805"
     CDS             complement(128..1162)
                     /gene="trpD"
                     /locus_tag="VF752_11805"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX8856271.1"
                     /translation="MPNDILTKAIDRLAHRGDLDVDEAALVMREIMEGRASDVQSAAL
                     LIALRTKGETVEEITGMARTMRELSRHVTVPNDDLLDTAGTGGGRPTFNVSTTAAIVA
                     AGAGCTVAKHGARSNTGPSGSADVLEALGARIDLDPEAVADCIEEIGFGFMFAPAHHT
                     AMRHVVPVRKELAVRTLFNFLGPLTNPAGATRQVIGVSAIEHIDTIARALANLGCRSG
                     LVVSSEDGLDEISVSGRTRVIEVREGQVESYTVAPGDLGVEPADSDAMRAGTPEANAQ
                     ITLTVLEGEPGPERSLVALNAGAAIYVGGRADSIEEGVRAAEQVLDSGAAREVLERYV
                     ARTRELSPQS"
     gene            complement(1162..1725)
                     /locus_tag="VF752_11810"
     CDS             complement(1162..1725)
                     /locus_tag="VF752_11810"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX8856272.1"
                     /translation="MKVLVVDNYDSFTYNLVQYLGELGADIDVVRNDALDADGLVARA
                     RGGKAIVSPGPCSPAEAGVSVEGIRALAEAGVPVLGVCLGHQALATAFGGRVVRGEPV
                     HGKTALVEHDGRTVFAGLETPLVAGRYHSLVVDPDLPDCLERSAHYGDTVMGVRHREL
                     PAEGVQFHPESVLTGEGKALLRNFLES"
     gene            complement(1722..>2473)
                     /locus_tag="VF752_11815"
     CDS             complement(1722..>2473)
                     /locus_tag="VF752_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX8856273.1"
                     /translation="VPSQRWSAPAPVEAFSIYRGLRTVNPSPYMYFLDFEDFELAGAS
                     PESLVKVTGRRAENRPIAGTRPRGDTVEEDVQRAEELLADEKERAEHVMLVDLGRNDL
                     GRVCEYGTVQVEELMAVETYSHVLHIVSSVSGRLREDVSALDALRACLPAGTLSGAPK
                     IRAMQIIDELEPVKRGPYGGAVGYISYTGDMDTCIYIRSAVVKDGQMHVQAGAGIVAD
                     SDPDYEVRETEAKARAVFGAVELACRQRDWA"
BASE COUNT          342 a          903 c          852 g          376 t
ORIGIN      
        1 tcgctgaagg ggcgtccgcc ctcgcgctgc gccgcctcgc gctcgagctc cgccaccggc
       61 cgctcgctct tgcgccgcgc cacgctctcg cgcgtggcct ggatcagatg ctcgaggcgg
      121 accgccatca gctctgcggg gagagctccc tcgtgcgcgc gacgtagcgt tcgagcacct
      181 cgcgcgcggc gcccgagtcg agcacctgct ccgcagcgcg cacgccctcc tcgatcgagt
      241 ccgcgcggcc gcccacgtag atggccgcgc cggcgttcag agcgacgagc gagcgctcgg
      301 gtccgggctc accctcgagc acggtgagcg tgatctgcgc gttcgcctcg ggcgtccctg
      361 cgcgcatggc gtcggaatcg gccggttcga cgccgaggtc gccgggcgcg accgtgtagc
      421 tctcgacctg gccctccctc acctcgatca cgcgggtgcg acccgagacg ctgatctcgt
      481 cgagcccatc ctcgctcgac actaccaatc cactccggca gccgaggttc gcgagagcgc
      541 gcgcgatcgt gtcgatgtgc tcgatcgcgg agacgccgat cacctgccgg gtcgcgcccg
      601 cggggttggt gagcgggccg aggaagttga acagcgtgcg cacggccagc tccttgcgga
      661 ccggcaccac gtggcgcatg gccgtgtggt gagcgggcgc gaacatgaag ccgaagccga
      721 tctcctcgat gcagtcggca accgcctctg ggtcgaggtc gatgcgcgcg ccgagcgcct
      781 cgagcacgtc ggccgaaccg gacgggcccg tgttcgagcg cgccccgtgc ttggccaccg
      841 tgcagccggc gcccgccgcg acgatcgctg cggtggtcga cacgttgaag gtggggcgcc
      901 cgccgcccgt gccggctgtg tcgaggaggt cgtcgttcgg aacggtcacg tggcgcgaga
      961 gctcccgcat cgtgcgggcc atccccgtga tctcctcgac cgtctcgccc ttggtgcgca
     1021 gcgcgatcag gagcgcggcc gactgaacgt cggaggcgcg cccctccatg atctcgcgca
     1081 tcacgagcgc cgcctcgtcc acgtcgaggt cgccgcggtg ggcgagccgg tcgatcgcct
     1141 tggtgaggat gtcgttcggc atcagctctc gaggaagttg cggaggagcg cctttccctc
     1201 ccccgtcagc accgactccg ggtggaactg cacgccctcg gcaggcagct ctcggtggcg
     1261 aacgcccatg acggtgtcgc cgtagtgcgc cgaacgctcc aggcagtccg gtaggtcggg
     1321 atcgacgacg agcgagtggt agcggcccgc cacgagtggg gtttcaaggc cggcgaagac
     1381 ggtcctgcca tcgtgctcca cgagcgcggt cttgccgtgc accggctcgc cgcgcacgac
     1441 gcgcccgccg aacgccgtgg cgagcgcctg gtggcccagg cacacgccca gcaccggcac
     1501 tcccgcttcc gcgagcgcgc gtatcccctc caccgagacg cccgcctcgg ccggcgagca
     1561 cggcccgggc gacacgatcg ccttgccacc gcgcgcgcgt gcgacgagcc cgtcggcgtc
     1621 gagcgcgtcg ttgcgcacca cgtcgatgtc cgcgcccagc tcgcccagat actggacgag
     1681 gttgtaggtg aacgagtcgt agttgtcgac gacgaggacc ttcatgccca gtcccgctgg
     1741 cggcaggcga gctcgaccgc gccgaagaca gcgcgcgcct ttgcctcggt ctcgcgcacc
     1801 tcatagtccg ggtccgagtc ggcgacgatc ccggcgccgg cctgcacgtg catctgcccg
     1861 tccttcacga ccgccgagcg gatgtagatg caggtgtcca tgtcgcccgt gtacgagatg
     1921 tagccgaccg cgccgccata tggtccgcgc ttcaccggct cgagctcgtc gatgatctgc
     1981 atcgcgcgga tcttgggtgc gccggagaga gtgcctgcgg gcaggcaggc tctgagcgcg
     2041 tcgagcgcgc tgacgtcctc gcgcaggcgc cccgacacag aggacacgat gtggagaacg
     2101 tgggagtagg tctccaccgc catcagctcc tcgacctgca ccgtgccgta ttcgcacacc
     2161 cgcccgaggt cgttgcggcc gaggtccacg agcatcacgt gctccgcccg ttccttctcg
     2221 tcggcgagca gctcctcggc ccgctgaacg tcctcctcga cggtgtcacc acgtgggcga
     2281 gtgccagcga tcggccgatt ctcggcgcgg cggccggtca ccttcacgag cgactccggc
     2341 gacgccccgg cgagctcgaa gtcctcgaag tcgaggaagt acatgtaggg gctcgggtta
     2401 accgtgcgca ggccgcggta gatcgagaac gcctccaccg gcgcgggggc gctccagcgc
     2461 tggctcggca cca
//
LOCUS       DASVXS010000211        22373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1231701, whole genome shotgun sequence.
ACCESSION   DASVXS010000211 DASVXS010000000
VERSION     DASVXS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22373
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231701"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VF752_11820"
     CDS             <1..859
                     /locus_tag="VF752_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX8856274.1"
                     /translation="VIGTELPRDKVVHGAHEACRALGADAEEFSRSIMTSDSGPKRAC
                     LEVLLPRGEVRLAAQAKGAGMISPRFATMFCFVQTDAQLDQATLDLLTGVCVERSFDR
                     ISVDGQLSTSDTVFAFANGASGVRIEPQTPDEIALGEALDALMRQLAIEMVADGEGAE
                     RVARVVVRGATELVDDVARAVANSPLVKTALLGADPNFGRILQAAGQAMAGRAPFYVD
                     LSIEGRECVSGGAVVDVSGEALRELEELVAKPEVEFELTLPGSGGETEVFFSDLSHGY
                     VSLNSEYST"
     gene            856..1698
                     /gene="argB"
                     /locus_tag="VF752_11825"
     CDS             856..1698
                     /gene="argB"
                     /locus_tag="VF752_11825"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX8856275.1"
                     /translation="MRDVATLLEALPYIREFHGKTVVIKYGGAAMTEPQLKEEFARDV
                     VLLKYVGLNPVVVHGGGPDITRYMERLGMDVRFVGGLRVSDEATVEVAKMVLVGKQNK
                     DIVLLLNRHGQPAVGLCGDDGLLFRVRKQQADTDEDLGFVGEIEHVNVDVLNHIAEDY
                     IPVIASVGADSEGHSYNVNADAAAGAVAAALGAYKVLFLTDVTGWMRDPERPDSLVSQ
                     ASADEVRAGLDGVSGGMRPKLEACLAAVDGGVVAAHIVDGRLEHSLLLELFTDQGIGT
                     KIRA"
     gene            1695..2849
                     /locus_tag="VF752_11830"
     CDS             1695..2849
                     /locus_tag="VF752_11830"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX8856276.1"
                     /translation="MSVSELQQLEREHVIGTYARNPVEFVRGEGTSLWDDEGNEYLDF
                     LAGISVVQIGHSHPALVEAVREQAGRLMHVSNLFYTEPGMRLAERLAASSLGGKVFFC
                     NSGAEANEAAIKLARRHKREGEIVVLSHGFHGRTYGALSATPQEAKQAPFAPLVPGFV
                     VVARDDPRALEQAVGERTAAVLIEPVQGESGIHPIAAEVLFTAREACDRHGALLIFDE
                     VQCGMGRTGTLWAYEQTGVRPDVMTVAKGLGGGLPIGACVTSPEYADVLQAGDHGSTF
                     AGGPVPCAAANAVLEVVNDESFLREVREKGEELVGGLRAIGAAGVRGLGLMLAFEVPD
                     APEVARRALLDERLVVNATGPTTVRLLPPLTVTREEIAEALRRLERLLAV"
     gene            2908..3126
                     /locus_tag="VF752_11835"
     CDS             2908..3126
                     /locus_tag="VF752_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856277.1"
                     /translation="MPKTSKDTAPQTDHGPVVEWTEEFDGQTVNFLQFREDIDATALL
                     KGLPDGQCQCPHLGYVLKGSMRFRVGDR"
     gene            3550..3969
                     /locus_tag="VF752_11840"
     CDS             3550..3969
                     /locus_tag="VF752_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856278.1"
                     /translation="MATNTELLHAGYDAWNRDDLDGWLELLDPDIEIRTAGVFPDLAP
                     VYRGHQRAAKFWRQMREPWQEFHIDVDQMEEDGDCVTAAIRFRARGHSGVDVDMRFGN
                     GIRVRDGVATELVNRRTREEAREALGPKPSRVPSERR"
     gene            4047..4529
                     /locus_tag="VF752_11845"
     CDS             4047..4529
                     /locus_tag="VF752_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856279.1"
                     /translation="MEHPVTPAEARAALETVEGGRLRVIDEIDLPSWYWWGLALCWIG
                     LGFIADLNHPWITAAATCAFGAAHSAVAPRVVSGRHRTRRLSVSAAVAGCATPRLVIG
                     GLIALAALTVALGFAANADGARHPATVASVVVAVVIVLGGPRLLAVVRRNAARSATAT
                     "
     gene            4526..4837
                     /locus_tag="VF752_11850"
     CDS             4526..4837
                     /locus_tag="VF752_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8856280.1"
                     /translation="MTVARFDELIHPSTRLSIVALLAAADWVEFAFVRDQLGLSDSAL
                     SKQFSTLEEAGYVSMERPVSDRRRRVRARLTPEGREAFQAHVAALRAIVGSADSASAV
                     L"
     gene            complement(4850..5185)
                     /locus_tag="VF752_11855"
     CDS             complement(4850..5185)
                     /locus_tag="VF752_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856281.1"
                     /translation="MASPTREDAQLMVQIAQWGTALGIGDAMGQVFADDFDPETADMS
                     DPPVRTILVFGESIGTLTKRDLLSAELVHDWLWVEGLWSRVGPAARRAREKFGEPRLY
                     ENFEALASG"
     gene            5260..6180
                     /locus_tag="VF752_11860"
     CDS             5260..6180
                     /locus_tag="VF752_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856282.1"
                     /translation="MELLATTGDAFVKVGAHERLQGSGAQCLALDGDTVYVGCRGSGL
                     KRSTDGGDTFEDVPFPEPDVFSVAVSAADGAVYAGTEPSRLFRSRDGGESWEELVALQ
                     EIPSRPEWSFPPRPWTSHVRWIAPSPHDAEVVLVGIELGGLMRTDDGGASFSDHRPEA
                     ARDVHCLAWHPSAPGRAYEAGGDGAAWSRDGGRTWDKADAGRDRRYAWALAVDSADPD
                     RWFVSAAPGPFDAHGSRPSNSAIYRWEGSGPWQVIEGPLDSHVYALATGDGQLFAGLG
                     DGRLLQSEDGGESWNELGERLGRISALAVR"
     gene            complement(6243..6698)
                     /locus_tag="VF752_11865"
     CDS             complement(6243..6698)
                     /locus_tag="VF752_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856283.1"
                     /translation="MIKITHSAVFVLDQDQALDFYVNKLGFEVNTDVDLGFMRWLTVN
                     APGQPDRQVLLELPGPPAHDPATAEQVRELLTKGATGFALGIQTDDVHKTYEEWKARG
                     VEFVDQPTERDYGIDCGLRDPFGNNLRLVQPVGGPSSAADPSELARQNG"
     gene            complement(6709..7131)
                     /locus_tag="VF752_11870"
     CDS             complement(6709..7131)
                     /locus_tag="VF752_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8856284.1"
                     /translation="MSRAVEDQNRRLLRARDAMDRSYAEPLDVPAVARVALMSEAHFI
                     RTFRETFGETPHRYLQRRRVERAMFMLRQTDRSVTEICFDVGFTSLGSFSRTFSAIVG
                     ESPSAYRSHSSPEAVPGCFTMVWTRPSSFGEAKVAAVD"
     gene            complement(7128..7676)
                     /locus_tag="VF752_11875"
     CDS             complement(7128..7676)
                     /locus_tag="VF752_11875"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8856285.1"
                     /translation="MTTWRRERSRVVYTNPWMTVREDDIERPDGSAGIYGVVEKPDFA
                     CVLPWDGERFWLVRQFRYPVDAPTVEFPQGSRDHHGEADEPSEELARRELKEETGLDA
                     TRWTWLGHLWKAVGYSSQGYDIYLATGLLEGEPDVSIEEQQIEVVQLTPAELERAIAS
                     GEVKDDTTVAALGLLRAKQAEV"
     gene            7712..8992
                     /locus_tag="VF752_11880"
     CDS             7712..8992
                     /locus_tag="VF752_11880"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX8856286.1"
                     /translation="MAERHERTILKKTASYEAEPGEVGRVLLLYSGGLDTSVMLKWIQ
                     DEYEAEVVALTVNLGQPGEEFDVVKGKALQLGALDCHVIDAREEFAENYVLPAIKANA
                     LYGGGYPLFTALGRPLIAKLAVQYARESGCDTIAHGCTGKGNDQVRIEATVAALAPEL
                     KIIAPVRGWQMGREEEIRYARENGIPVKGGTEAPPYSIDDNLWGRSSEGGPIEDIGEP
                     PRDDVFNLVTKPEDAPDEAQLVEIEFERGRPVAIDGERVGLVELLERAGEIGARHGVG
                     IVDHIEDRIVGLKVRDLYEVPAAEIILTAHKELEKLVGTIHQNNFKPGLDHQWAFLVY
                     AGLWQEPLLGDLNAYMDSVNEQVTGGITLRLYKGKVTPVARRSPNALYDPELAGFGES
                     GGLFSQQASPGFIELWSLQSRMAYAIRNRGRSEE"
     gene            8989..9159
                     /locus_tag="VF752_11885"
     CDS             8989..9159
                     /locus_tag="VF752_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856287.1"
                     /translation="MSTKAYTFLGRVVWHSAMVFGKWKFQQNRAKIRAAGVVALVLLG
                     GVAAAKASSSGD"
     gene            complement(9156..9638)
                     /locus_tag="VF752_11890"
     CDS             complement(9156..9638)
                     /locus_tag="VF752_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856288.1"
                     /translation="MFRKGPIPAWAHGLLEYAAAVLFIVAPFLFSFDDNGAKAVSIAA
                     GVVVLLFAGFSEFSTGLSKSIPIAAHALLDYLLAILFVAMPFLVGFDDETAPTVFFIA
                     MGVFWLLLSIATRYAPEGLRQPREPRPAERERRRFRRGEDLTDVPEFEVPPREPEQRR
                     "
     gene            complement(9656..10009)
                     /locus_tag="VF752_11895"
     CDS             complement(9656..10009)
                     /locus_tag="VF752_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8856289.1"
                     /translation="MDLSLVNFDNPTETRTFEKGRFEVYEVGPMTLGRATYEPGWRWS
                     EHVGPASGESSCQVEHVGLVLSGQAAAKMDDGREVVMRAGDFFYIPPGHDSWVVGDEP
                     YVSLHIMGSERYAVS"
     gene            complement(10009..10404)
                     /locus_tag="VF752_11900"
     CDS             complement(10009..10404)
                     /locus_tag="VF752_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856290.1"
                     /translation="MAAPFTQLGRVVVLVDDQDAALAFYRDVLGFEVLHDSEDGGFRY
                     LHVGLPGWGGPGIWLMPANDSGTRSLVGRQAGAHPLLVLYTDDIEATRTGLEAAGVKV
                     WQDRDDDGSRSFHFRDVSGNTLIAVQLTQ"
     gene            10470..13169
                     /locus_tag="VF752_11905"
     CDS             10470..13169
                     /locus_tag="VF752_11905"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8856291.1"
                     /translation="MSEQIGISVNGQAHEIDVRDDESAVEVLRERLGLTGTKLVCGEG
                     VCGACTVLVDGVPLASCLLPATALDGRTVTTIEGFGPELHPVQRAFVANDALQCGYCT
                     PGFVVEAIAFHDRWRSERGTEEPTREDVIAALAGHLCRCGAYVGIIAAVRDACAGRFD
                     AADPPAPRVEARVKVTGRAIYTADVRQDGQLEGVILRSPHAHARVLLLDLDAARAVDG
                     VRAAVDLLDDERVVRYVGQEVAAVAAVDRSTAEQAVERIAVEYDSLPAAIGMEAARDS
                     GAPQVYTGLRRRAPSSAEGPLFPARWRGNVRGPSSFSKRGRAARRLLTQARADGDPLL
                     VEGRWRTAAQVHTAFEPHACVARWDDGSLQAHVSTQAAAHLAHQIAERFELPEERVRV
                     IAEHVGGGFGAKLALTQEIVAAIELARAARAPVRVVLGRHEELAVAGYRPPAELDLAL
                     LASRSGGLRALSVRAHADSGVAVGSSIAALCRFIYPAPAIELLDHDVVNHMPPGKPFR
                     GPGAPVAAWALEQAVDEAAHRLGEDPLALRRRLDPDPLRQRLYERAQGLDVWRGRSQT
                     GLQSGRFRRGVGVAAGNWVYVYQLDCTVEVGIEGGRLFVATATQDMGTGSRSVLADVV
                     GRAFGLESADVEVRIGDSRQLRGPTSGGSRTTATIVPAALEAVARLRAQIVQALGLRD
                     AREDDTGIRHAAGSISWKEAIASAPDVTAVADRPGDDHTASNAAGPLAGAGPAGAVMR
                     WAMRRSMTLEVGRGSTGAVHVSEIEVDTMLGKVRVLRVAAGVAAGRIAAPALARSQCY
                     GGVIQGIGYGLYEQRQADTRDGLVLTAGLEDYRIPGIGDVPEIDIHFMEDGFEHVPGG
                     GVGLGELSTIAVAASVGNAVYNATGWRPRELPIRPDRLVAGLS"
     gene            13166..14110
                     /locus_tag="VF752_11910"
     CDS             13166..14110
                     /locus_tag="VF752_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX8856292.1"
                     /translation="MSASPPTIEGALAATGEYRAGGTDLQQRLRSGVSHGPVIDLAGL
                     PGLDAIEWDEQGRATIGALAPIAALATDPRVLASYPGLALSAGALATPQIRAAGTLGG
                     NLLQRNRCWYFQNPGVNCLKKGGDDCPARSGNHLYGVCFDLGPCVAPHPSTLGMALLA
                     YGAEVDVAGGGTRSVAALYGDGSDGRHDHQLREHELLTAVLMPEPLEGERAAYFRAIS
                     RALAEWPLVEAVVRLVVADGTVRFARVAVGGVAPVPLRLESVEEALLNRPASPETFEE
                     AVSSATQGARPLPMTRYKLELLPGTVRETLERAVAAGP"
     gene            14279..17776
                     /gene="dnaE"
                     /locus_tag="VF752_11915"
     CDS             14279..17776
                     /gene="dnaE"
                     /locus_tag="VF752_11915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8856293.1"
                     /translation="MSPSPAVHLHVHSEYSLLDGACNIENLAARAAEYGQPALGLTDH
                     GVMNGAVEFYKACKKHGVKPIIGFEAYLVDDLKSDAVKYERNHLTLLASSAEGFRNLV
                     KLTSAGFLEGYKRGKANVDLDLLSRHSKDVIALTGCLQSRFCRRLVEDNPAEAREHVD
                     DLMRIFGPENVYFEVQKNGLAEQEKANEGIVRAAREFGRPLVGTADVHYLRREDYDHH
                     KALLCVQTKSTLLEPKLSFDTNEFYLKSSEEMAASFGEWPEAIASTLEIAERCDVELE
                     LGKMLIPRYPTPDGSDEGVYLRRLAEEGLRRRYGDPVPAEATERLNMELEVIDRMGFN
                     AYFLIVWDFVKFAKDNGIAVGPGRGSAAGSLVSYTLNITDVDPLRYDLLFERFLNAER
                     VSMPDIDIDFSVKGRDRVIRYVTEKYGTESVAQIITFGRMFPRAATRDAARLLGYDYG
                     AGDRLAKLIPDPIMGRPPSFDDCLKPGEELAKAYAEDPQAKAIIDVARGLEGTVRNNS
                     IHAAAVVIADRPLTDIVPLQLAEDRGVVDEDGNRSYRTVTQYSMKPIEEIGLLKMDFL
                     GLRNLDVIESALDIIERSLGERPDMTTLPLDDAKTYEMLARGDSTGVFQFESEGMREA
                     LKKVRPTEFEDLVALNALYRPGAMRHIDTYARNKRNPEAVTYIDDRLRPITESTYSVI
                     LYQEQSMQIAKGIAGFSGPEADDLRKAIGKKDRAKMASLKQRFVEGARATGTSEAVIE
                     QLWQTNEAAADYSFNRSHAACYALIGYRTAWLKANYPAEYMAALISSVMSTKDKVPFF
                     VSRCEEMGIEVLPPDVNESGHDFVVVEGHIRFGLDAVKNVGSAAVDAILEAREQGGPF
                     ESIWDFCERVDCRAVNKKAIEALIKCGALDSTGASRRGMLEIMPQAQASGQKAQEDAL
                     LGQGSIFDFDEPSAGGGGPAPFQAARPVPNLSDERSDLNLMEKETLGLFLSSHPLKEV
                     RAALRAKVECSIAELADKKDGDWVTVGGMISEAKRIRTKKGDPMMFATLDDLEGQVEI
                     LVFNSAYSENAEKIDVDSVVVVRGRVDHKEQGETKLVVQSVELFEATPAEIAKAQEDA
                     ANMVPPPPKRITLEVPGSVSESFLDELLEVVSHHRGEYELCLKIGPRALLLGPDHRVS
                     GTSSCRSELENLVALAQAA"
     gene            18015..18491
                     /locus_tag="VF752_11920"
     CDS             18015..18491
                     /locus_tag="VF752_11920"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX8856294.1"
                     /translation="MTSHSSTQTPGTNGRRRSRVDQLVRSTLPQKLPPPANPALLKHA
                     EERADNAQNRLADRITAFSGSMLFVYLHILWFACWIGFGVEKYPFGLLTMIVSLEAIF
                     LSTFVLISQNRADAKRQVQANEQWRTVQEEDRQNDELLSLSKEILKLTQEVHSRQS"
     gene            18586..18978
                     /locus_tag="VF752_11925"
     CDS             18586..18978
                     /locus_tag="VF752_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856295.1"
                     /translation="MDGAVQERIAHECRQCCAFCDRLVQPAGCIEANCPYLYVYDDEQ
                     TGGRYMGCLNKVFRGEIDVELFREAEKTRHGFGGVKMSGRPTSFCRFTVERAYHGEGE
                     AFDCVNPEFFEGPEPVDGLHSFDLRDRL"
     gene            19094..22357
                     /locus_tag="VF752_11930"
     CDS             19094..22357
                     /locus_tag="VF752_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX8856296.1"
                     /translation="MLHASARACRAPRMVARFVSLAAVAMLVTAAAPAVARAADYVAM
                     GDSYSSGTGTREYYEGCERSVYAYPYLIKGSFGSSFSFVACGGAKTQDVLNNQVSALS
                     SGTEFATISIGGNDAGFSSVITKCAQPIVSCDGDINNAQSFIQNTLPGRLDNVYNQIR
                     SRAPNAVVGVVGYPRLFNGEDCNLGTFFSSSEMTRLNQTADLLANVTRARAQAHGFTF
                     VDARPAFIGHAVCDNVEWLNGLSNPTSESYHPNRTGHSSGYASIVRAALLAAPDPNAP
                     VGGNGRIAFVSTRGGNSDVWVMNNNGQFPMNLTNAPGEDVDPVWSADGTQIAFASNRD
                     GDNEIYVMNGDGTGVTKLTNNTNDDRDPTWSPNGDYIAFKSDRTGNNEIFRMTSSGGS
                     QTNLTNNSASDFSPDWSPDGAEVVFQRFTNGSATGNGNEVFKMNADGQGQTNLTNNAA
                     SINDGSPAWSPDGTTLAFHSNRDGDFEVFTMPAIGGSATQRTSNTASDGEPAWAPNGT
                     QLAFQSNRDGNNNDIFTMTSSGGSQTNRSSNPATDVSPSWQADSTPPLTSITVGPNGP
                     TNTSTPQFEFTSSELGSNLQCRLDNGSFQTCPSPFTTASLADGPHTFSARSVDPAGNV
                     DPNPVVRTFLVDTKAPSLSVECPDSMLLNAPGSAIVTASDAGSGLPPGEDPSGSYPLD
                     TSQPGSQSFRIDAFDLAGNQASAECDYEVLYPAPGTPDVTSGGNPNAGAFTLSWVPAA
                     PPEYGLHYTLERRDADDDAWQQAASGIDGTDYSFTAGDSADEGTWSYRVKGSDGDHET
                     PWSEVSASVKVDQTAPAKPAIVPDRAPDYAGDGGWYRDSVLVSTEDRGDPTLRDGSDP
                     TGVDASTVAGPELLDASGTVHRTVSDRVGNTSDEATEDFQVDTEKPSLQLDCPEDVVL
                     GGEASVEVTAGDSQSGLASDPSGAVEVDTSHVGTQTIERTATDNVGHERTKSCHVLVR
                     YDYGGLLQPVNRDGSSVFKLGSTIPLKLRLSDHAGNSVAGAHVEVQLERTSTSVDGTE
                     FEEVVDATPTIGKTFEYSDGSEVYRFNLSTKPLSAGTWRIRISLDDGTVHRTQISLR"
BASE COUNT         3609 a         7534 c         7877 g         3353 t
ORIGIN      
        1 cgtgatcggc acggagctgc cgcgcgacaa ggtggtgcac ggcgcgcacg aggcgtgtcg
       61 cgcgcttggc gccgacgccg aggagttctc ccgctcgata atgacgagcg acagcgggcc
      121 gaagcgcgcc tgcctcgagg tcctcttgcc gcgaggagag gtcaggctgg ccgcccaggc
      181 caagggcgcg gggatgatct cgccgcgctt cgccacgatg ttctgcttcg tgcagaccga
      241 tgcgcagctc gaccaggcga cgctcgacct cctcacaggc gtgtgcgtgg agcgctcgtt
      301 cgaccggatc agcgtcgacg gccagctctc cacgagcgac acggtctttg cgttcgcgaa
      361 cggcgcctcc ggcgtgcgaa tcgagccgca gaccccggac gagatcgccc ttggagaggc
      421 gctcgacgcg ctgatgcggc agctggcgat cgagatggtg gccgacggcg agggcgcgga
      481 gcgcgtggca cgggtcgtcg tgcgcggcgc gaccgagctt gtggacgacg tcgcgcgcgc
      541 agtggccaac tcgccgctcg tgaagacggc gctgctcggc gccgacccca acttcggacg
      601 aatcctccaa gccgccgggc aggcgatggc cggccgggcg ccgttctacg tcgacctcag
      661 catcgagggc cgcgagtgcg tgtcgggcgg tgccgtggtg gacgtgagcg gcgaggcact
      721 gcgcgagctc gaggagctcg tcgcgaagcc ggaggtggag ttcgagctca cgctgccggg
      781 cagcggcggc gagaccgagg tcttcttctc cgacctctcg cacggctacg tgtcactcaa
      841 ctcggagtac tcgacatgag agatgtcgca accctgctcg aggcgctgcc gtacatcagg
      901 gagttccacg gcaagacggt cgtgatcaag tacggcggcg cggcgatgac cgagccgcag
      961 ctcaaggagg agttcgcccg cgacgtggtg ctgctcaagt acgtcgggct gaacccggtg
     1021 gtcgtgcacg gcggcgggcc tgacatcacg cgctacatgg agcggctcgg catggacgtg
     1081 cgcttcgtcg gcggcctgcg ggtgtccgac gaggccaccg tcgaggtcgc gaagatggtg
     1141 ctcgtcggca agcagaacaa ggacatcgtg ctgctcttga accgccacgg gcagccggcg
     1201 gtcggcctct gcggcgacga cggcctgctg ttccgggtgc gcaagcagca ggcggacacc
     1261 gacgaggacc tcggtttcgt cggggagatc gagcacgtga acgtggacgt gctcaaccac
     1321 atcgccgagg actacatccc ggtgatcgcg tcggtgggcg ccgattccga gggccactcc
     1381 tacaacgtga acgccgacgc cgccgccggc gctgtggcgg ccgcgctcgg cgcctacaag
     1441 gtgctcttcc tcaccgacgt caccggctgg atgcgcgacc ccgagcgccc ggacagcctc
     1501 gtgtcgcagg cgagcgcgga cgaggtccgc gccggcctcg acggcgtgag cggggggatg
     1561 cgcccgaagc tcgaggcctg cctcgcggcg gtggacgggg gcgtcgtcgc ggcgcacatc
     1621 gtggacggcc ggctcgagca ctcgctgctg ctcgagctgt tcaccgacca gggcatcggc
     1681 acgaagatcc gcgcgtgagc gtctcggagc tgcagcagct cgagcgcgag cacgtgatcg
     1741 gcacgtacgc gcgcaacccc gtcgagttcg tgcgcggcga gggcacgagc ctgtgggacg
     1801 acgagggcaa cgagtacctc gacttcctcg ccgggatctc ggtcgtgcag atcggccact
     1861 cgcatccggc gctcgtggag gccgtgcgcg agcaggccgg gcggctcatg cacgtctcga
     1921 acctgttcta caccgagccg ggaatgaggc tcgccgagcg gctcgccgcg tcgtcgctcg
     1981 gcggcaaggt gttcttctgc aactccggcg cggaggcgaa cgaggcggct atcaagctcg
     2041 cgcgccgcca caagcgcgag ggcgagatcg tggtgctctc gcacgggttc cacggtcgca
     2101 cctacggcgc gctgtcggcc actccacaag aggcgaagca ggcgccgttc gcgccgctcg
     2161 tgccgggctt cgtggtggtg gcccgcgatg acccacgcgc gctcgaacag gcggtcggcg
     2221 agcgcaccgc tgcagtgctg atcgaacccg tgcagggcga gtcgggcatc cacccgattg
     2281 cggccgaggt cctgttcacc gcccgcgagg cgtgcgaccg ccacggcgcg ctgctcatct
     2341 tcgacgaggt gcagtgcggg atgggacgca ccggaacgct gtgggcgtac gagcagacgg
     2401 gtgtgcggcc cgacgtgatg acggtcgcga agggcctcgg cggcgggctc ccgatcggcg
     2461 cctgcgtcac ctcccccgag tacgcggacg tgctccaggc gggcgaccac ggctcgacgt
     2521 tcgccggcgg cccggtgccg tgcgcggctg cgaacgcggt gctcgaggtc gtcaacgatg
     2581 agtcgttcct gcgcgaggtt cgcgagaagg gggaggagct ggttgggggc ctgcgcgcga
     2641 tcggcgcagc gggcgtgcgc ggcctcggac tgatgctcgc gttcgaggtg cccgacgcgc
     2701 ccgaggtggc gcggcgcgcg cttctcgacg agcgcctcgt ggtgaatgcg accgggccga
     2761 cgaccgtgcg gttgctgccg cccctcaccg tgactcgcga agagatcgcg gaggcgctac
     2821 ggcgcctcga gcggctgctc gcggtctgat tgacccagtt gtccgcgggc ggttagcgtc
     2881 gcttccagcg accgaaggag ggcggagatg cccaagactt caaaagacac cgcgccgcag
     2941 acggaccacg ggccggtggt cgagtggacc gaggagttcg acggccagac cgtgaacttc
     3001 ctccagttcc gcgaggacat cgacgcgacg gcgctcctca aggggctgcc ggacggtcag
     3061 tgccagtgcc cgcatttggg ctacgtgctc aaggggagca tgcgattccg ggtcggtgac
     3121 cgctaggaga tattcgacgc cggcgacgcc ttctaccttg ggccgggcca catccccgtc
     3181 ggcaacgccc caggcacgga gtacctccag ttcagcccga gcgagcagct caaggaggtc
     3241 gccgacacca tcactcggaa catgcaggag atgcagtccc aggcgggcgg gtagccgctc
     3301 agccacgaaa tgacgtatgg cgggcaaacg tccggaagct cctgacgact gaccgagtcc
     3361 cgacgaaata catgctcctg agcgcgggca aactgaggag gtgcgcgcgg ggggcgctga
     3421 cgatacggcg caactcctga ctttggttct cctatgcgga accaacctca ggaggtgccg
     3481 ccaggcgcgc gcgaggcttg cccacgcggg cactcttctc atctgtgcca tttgcgatcc
     3541 tgggaggcga tggcgactaa caccgagctg ctccacgccg ggtacgacgc gtggaatcgc
     3601 gacgacctcg atggctggct ggagcttctc gatcccgaca tcgagatccg tacggcgggc
     3661 gtctttccgg acctcgctcc ggtctaccgc gggcaccaac gggccgccaa gttctggcgc
     3721 cagatgcgcg agccgtggca ggagttccac atcgatgtcg accagatgga agaggacggc
     3781 gactgcgtga cggcggcgat caggtttcgc gcccgcggcc acagcggggt cgacgtcgac
     3841 atgcgcttcg gcaacggcat ccgggtgcgg gacggcgttg cgaccgagct cgtcaaccgg
     3901 cgcacgaggg aggaagcccg cgaggcgctg ggtccaaagc cgtcgcgtgt gcccagcgag
     3961 cggcgctgat tcgccggcgc cgcttggttg actttcctgc tcggaaagtt atgctctgct
     4021 ttccaacttg gaaagtgagc cccttgatgg aacacccagt cacaccagcc gaagctcgcg
     4081 cagcgctcga gacggtcgag ggcggccggc ttcgcgtcat cgacgagatt gatctgccca
     4141 gctggtactg gtggggcctg gcgctctgct ggatcgggct cggcttcatc gccgacttga
     4201 accatccttg gatcaccgcg gcggccacgt gtgcctttgg ggccgcccac tcggcagtcg
     4261 ccccgcgcgt cgtgagcggc cgccaccgga cccgccgcct gagcgtcagc gccgccgtcg
     4321 ccggctgtgc cacgccgcgt ctggtgatcg ggggcctgat cgcgctcgcg gcactcacgg
     4381 tcgctctcgg atttgccgcg aatgccgatg gcgctcgcca cccggcaacc gtcgccagcg
     4441 tcgtcgtcgc cgtggtcatc gtgctcggcg gcccccgcct tctcgcggtg gtccggcgca
     4501 acgcggccag gtccgcgacg gcaacatgac cgtcgcccgt ttcgacgagc tgatccaccc
     4561 gagcacgcga ctgtcgatcg tcgcgttgct cgcggctgcc gactgggtcg agttcgcgtt
     4621 cgtccgggac cagctcggtt tgtccgactc ggcgctgtcg aagcagttct ccaccctcga
     4681 ggaggcaggc tacgtgagca tggagcggcc cgtcagtgac cgtcgaaggc gcgtgagggc
     4741 gcggctcact cctgaaggca gggaggcgtt tcaggcgcat gtcgcggcgc tacgcgcgat
     4801 cgtgggaagc gctgactccg cgagcgcggt gctttaagcg gttctccggt tacccgctgg
     4861 ccaaggcctc gaagttctcg tagagccgag gctcgccaaa cttctcgcgc gcccgacgag
     4921 ctgccggccc cacgcgcgac cagagcccct cgacccacag ccaatcatgg acgagctcgg
     4981 cactcagcag gtcgcgcttc gtcagcgtgc cgatcgactc accgaacacg aggatcgttc
     5041 ggaccggcgg gtcgctcatg tcagcggtct ccgggtcgaa gtcgtccgcg aatacctgtc
     5101 ccatggcatc tccgatgcca agcgcggtgc cccactgggc gatctggacc atgagctgtg
     5161 cgtcttcacg ggtcggcgaa gccatgattt gcctcctcat cggcgctgca tcggtgtcga
     5221 agccagcgca ccatagaccg atgcgcgatt ctgtacgcga tggaactgct tgcgacgacc
     5281 ggtgatgcgt tcgtgaaggt aggcgcgcac gagcgcctgc agggttcggg ggcgcagtgc
     5341 ctggcgctcg acggggacac cgtctacgtg gggtgtcgcg gaagcgggct gaagcgcagc
     5401 accgacggcg gcgacacttt cgaggacgtg ccgttcccgg agccggacgt gttcagcgtc
     5461 gcggtgagcg cggcggacgg cgcggtgtat gcgggaacgg agccgagccg gctgttccgc
     5521 agccgcgacg gcggcgagag ctgggaggag ctggtcgcgc ttcaggagat cccgtcgcga
     5581 ccggagtgga gctttccgcc gcgcccctgg acctcgcacg tgcgctggat cgcaccgagt
     5641 ccgcacgatg ccgaggtggt gctggtaggg atcgagcttg gcgggctgat gcgcacggac
     5701 gacggcggcg cctcgttcag cgaccaccgg cccgaggccg cccgcgacgt gcactgcctc
     5761 gcctggcatc cgagcgctcc gggccgcgcc tacgaggccg gcggcgacgg cgcagcatgg
     5821 agccgcgacg gcggccgaac ctgggacaaa gcggacgccg ggcgagaccg ccgctacgcc
     5881 tgggccctcg ccgtcgactc ggccgatccg gaccgctggt tcgtctcggc tgcgcccggg
     5941 ccattcgacg cacatggcag ccgcccatcc aactccgcga tctaccgctg ggagggctct
     6001 ggcccctggc aggtgatcga gggcccgctc gactcgcacg tctacgcgct cgcgacggga
     6061 gacggccagc tcttcgcggg cctgggcgac ggcaggctgc tccagagcga ggacggcggt
     6121 gagagctgga acgagctggg ggagcggctc ggccggatca gcgcgctggc cgtgaggtga
     6181 acgcaaagcg gcgggccgac ggctggcgcc gtgacccgcc gcctcacgtg cacaagggtc
     6241 cgtcagccgt tctggcgagc gagctccgag gggtcggcgg ccgaggacgg accgccaacc
     6301 ggctgcacga gccggaggtt gttgccgaac ggatcgcgga gaccgcagtc gatgccgtag
     6361 tcccgctcgg tcggctggtc gacgaactcc acgccgcgtg ccttccactc ttcgtacgtc
     6421 ttgtggacgt cgtcggtctg gatcccaagt gcgaagccgg tcgcaccctt cgtcaggagc
     6481 tcacgcacct gctccgcggt cgccgggtcg tgcgcgggcg ggccggggag ctcgagcagc
     6541 acctggcggt ctggctggcc aggtgcgttg accgtgagcc agcgcatgaa tcccaggtcg
     6601 acgtcggtgt tgacctcgaa cccgagcttg ttgacgtaga agtcgagtgc ctggtcctgg
     6661 tcgagtacga agacggctga gtgagtgatt ttgatcatgc cggcgacgct aatcgacggc
     6721 ggccaccttg gcttctccaa aactgctcgg tcgcgtccag accatcgtga agcagcccgg
     6781 cacggcctcc ggcgacgaat gcgaccgata ggcgcttggc gattcgccca cgatcgcgct
     6841 gaaggtgcgg ctgaagctgc cgaggctcgt gaagcccacg tcgaagcaga tctcggtcac
     6901 gctgcggtcg gtctggcgaa gcatgaacat cgcgcgttcc acgcggcgcc gctgcaggta
     6961 gcggtgaggc gtctcgccga aggtctcgcg gaaggtgcgg atgaagtgcg cctctgacat
     7021 cagcgcgacg cgcgccaccg cgggcacgtc gagcggctcg gcgtagctcc ggtccatcgc
     7081 gtcgcgggcg cggagcagcc ggcggttttg atcctcgacc gcccggctca tacctcggcc
     7141 tgcttggcgc gcagcagtcc gagcgccgcg accgtcgtgt cgtccttcac ctcgcccgac
     7201 gcgatcgccc gctcgagctc ggcgggcgtc agctggacga cctcgatctg ctgctcctcg
     7261 atgctcacgt cgggctcgcc ctcgagcagg ccggtggcga gatagatgtc gtagccctgc
     7321 gacgagtaac cgaccgcctt ccagaggtgg ccgagccagg tccagcgggt cgcgtcgagg
     7381 ccggtctcct ccttcagctc gcgccgggcg agctcctccg acggctcatc ggcctcgccg
     7441 tggtggtcgc ggcttccctg cgggaactcg accgtcggcg cgtcgaccgg atagcgaaac
     7501 tgccgcacga gccagaagcg ttcgccgtcc cagggcaaga cgcacgcgaa gtcgggcttc
     7561 tcgaccacgc cgtagatgcc ggcggagccg tcggggcgct cgatgtcgtc ctcgcgcacg
     7621 gtcatccacg ggttggtgta gacgaccctc gacctctccc tgcgccatgt ggtcacccga
     7681 cggatgttag gtgttagctt cccgcgccca gatggccgag cggcacgagc gcacgatcct
     7741 gaagaagacc gcctcctacg aggcagaacc gggcgaggtc gggcgcgtcc tgctgctcta
     7801 ctccggcggc ctggacacga gcgtgatgct caagtggatc caggacgagt acgaggccga
     7861 ggtcgttgcg ctcaccgtca acctcggcca acccggcgag gagttcgacg tggtgaaggg
     7921 aaaggcgctc caactcggag cgctcgactg ccacgtgatc gacgcgcgcg aagagttcgc
     7981 cgagaactac gtgctgccgg cgatcaaggc gaacgcgctc tacggcggcg ggtatccgct
     8041 cttcaccgcg ctcggccgcc cgctgatcgc gaagctcgcg gtgcagtacg cgcgcgagtc
     8101 gggttgcgac acgatcgcgc acggctgcac tggcaagggc aacgaccagg tgcggatcga
     8161 ggccacggtg gcggcgctcg cgcccgagct gaagatcatc gcgcccgtgc gcggctggca
     8221 gatggggcgc gaggaggaga tccgctacgc gcgcgagaac ggcatcccgg tcaagggcgg
     8281 gaccgaggcg ccgccgtatt cgatcgacga caacctctgg ggccgctcgt ccgagggcgg
     8341 cccgatcgag gacatcggcg agccgccgcg cgacgacgtc ttcaacctcg tcacgaagcc
     8401 cgaggacgcg cccgacgagg cgcagctcgt ggagatcgag ttcgagcgcg gccgcccggt
     8461 ggcgatcgac ggcgagcgcg ttggcctcgt cgagttgctc gagcgcgcgg gggagatcgg
     8521 cgcccgccac ggcgtcggca tcgtcgacca catcgaggac cgcatcgtcg ggctcaaggt
     8581 gcgcgacctc tacgaggtcc cggccgcgga gatcatcctc accgcccaca aggagctcga
     8641 aaagctcgtc ggcacgatcc accagaacaa cttcaagccg gggctcgacc atcagtgggc
     8701 gttcctcgtc tacgccggcc tctggcagga gccgctgctc ggcgacctga acgcgtacat
     8761 ggactcggtc aacgagcagg tgaccggcgg gatcacgctg cggctctaca agggcaaggt
     8821 gacgccggtg gcgcggcgct cgccgaacgc gctctacgac cccgagcttg ccggcttcgg
     8881 cgagtccggc ggcctcttct cgcaacaggc gagcccgggc ttcatcgagc tctggagcct
     8941 ccagtcccgc atggcgtacg ccatcaggaa ccggggtagg agcgaggagt gagcacgaag
     9001 gcctacacat tcctgggccg ggtcgtgtgg cacagcgcca tggtgttcgg caagtggaag
     9061 ttccagcaga accgcgcgaa gatccgcgcc gccggcgtgg tcgcgctcgt gctgctcggc
     9121 ggagtggccg ccgcgaaagc ttcctcttcc ggcgactagc gccgctgctc cggctcgcgc
     9181 ggaggaacct cgaactccgg cacgtcggtg agatcctcgc cgcgccggaa cctccgacgt
     9241 tcgcgctcgg cggggcgcgg ctcccgcggc tggcgcaggc cctccggcgc gtagcgagtg
     9301 gcgatcgaga gcaggagcca gaacacgccc atcgcgatga agaacaccgt cggggccgtc
     9361 tcgtcgtcga agccgacgag gaacggcatt gcgacgaaga ggatcgcaag caggtagtcg
     9421 agcagcgcat gggcggctat cgggatcgac ttcgagagac cggtcgagaa ctcgctgaag
     9481 ccggcgaaca ggagcacgac gacgccggcg gcgatcgaga ccgccttggc gccgttgtcg
     9541 tcgaagctga acaggaacgg cgccacgatg aagaggactg cggccgcgta ctcgagcaga
     9601 ccgtgggccc acgcggggat tggtcccttg cggaacatgg tttgacgtta cccgttcagc
     9661 tcacggcgta gcgctcgctg cccatgatgt ggagcgagac gtaaggctcg tcgccgacca
     9721 cccagctgtc gtggcccggc gggatgtaaa agaagtcgcc ggcgcgcatc acgacctccc
     9781 ggccatcgtc catcttcgcc gccgcctgtc cgctcaggac caggcccacg tgctcgacct
     9841 ggcacgagga ctcgccgctg gccggcccca cgtgctcgct ccagcgccag cccggctcgt
     9901 aggtcgcgcg gccgagcgtc atcgggccta cctcgtacac ctcgaaccgg cctttctcga
     9961 aggtgcgcgt ctcggtggga ttgtcgaagt tgacgaggga gaggtccatc actgcgtgag
    10021 ctgcacggcg atgagcgtgt taccggagac atcgcgaaaa tgaaaggagc ggcttccgtc
    10081 gtcgtcgcgg tcctgccaca ccttgactcc ggccgcctcg agtccggtgc gggtcgcctc
    10141 gatgtcgtcg gtgtagagca ccaacagggg atgtgcgccc gcttggcgcc ccacgagcga
    10201 gcgcgtgccc gaatcgtttg ccggcatcag ccagattccg ggcccgcccc aaccgggaag
    10261 cccgacgtgc aggtagcgga agccgccgtc ctcggagtcg tgcagcacct cgaagccgag
    10321 cacatcccgg tagaaggcga gtgccgcgtc ctggtcgtca acgagcacca ccacgcgccc
    10381 gagctgggtg aacggagcgg ccatggccgc gcagtatctc cgaacttgac cggcgtacgg
    10441 ctcgcgcgcg agagtctccc cagcggcgag tgagcgagca gatcgggatc agcgtcaacg
    10501 gccaggcgca cgagatcgac gtgcgcgatg acgagtctgc ggtcgaggtg ctgcgcgagc
    10561 gtctcgggtt gaccggaaca aagctcgtct gcggggaggg tgtctgcggg gcgtgcacgg
    10621 tccttgttga cggcgtgccg ttggccagct gtcttctccc ggcgactgcg ctcgacggcc
    10681 ggacggtcac gacgatcgag ggattcggtc ctgaacttca tcccgtccag cgggcgttcg
    10741 tcgccaacga tgcgctgcag tgcgggtact gcacgcccgg cttcgtcgtc gaggcgatcg
    10801 ccttccacga ccgctggcgc tccgagcgcg gcaccgagga gccgacgcgg gaggacgtaa
    10861 ttgccgcgct ggcaggccac ctgtgccgct gtggagccta cgttggcatc atcgccgcgg
    10921 tgcgcgacgc ctgcgcgggc cgcttcgacg cagccgatcc gcccgcgccg cgcgtcgagg
    10981 cgcgcgtgaa ggtcaccggg cgggcgatct acacggccga cgtccgccag gacggacagc
    11041 tcgagggtgt gatcctgcgt tcgccccacg cacacgcccg cgtcctcttg ctcgacctcg
    11101 atgcggctcg cgcggtcgac ggagtgcgcg ccgccgtcga cctgctcgac gacgagcgcg
    11161 tcgttcgcta cgtggggcag gaggtcgcgg ctgtcgccgc ggtcgaccgg tccaccgccg
    11221 agcaggcggt cgagcggatc gctgtggagt acgactcgct tcccgccgct attgggatgg
    11281 aagcggcacg ggacagcggc gcgccgcagg tctataccgg gctgcgccga cgggcgccca
    11341 gctccgccga agggccgctg ttcccggcca ggtggcgggg gaacgttcgc ggtccctcga
    11401 gcttctcgaa gcgaggtcgg gccgcgcgcc ggctcctcac acaggctcgt gcagacgggg
    11461 accccctgct cgtcgagggc cgctggcgga ccgcggcgca ggtgcacacc gcgttcgagc
    11521 cccacgcgtg cgtcgctcgc tgggacgacg gctccctgca ggcgcacgtg tcgactcagg
    11581 ccgcggcgca cctggcacac cagatcgcgg agcgcttcga attgccggag gagcgcgtgc
    11641 gcgtgatcgc cgaacacgtg ggcggtggct ttggcgcgaa gctcgcgctg acccaggaga
    11701 tcgtggcggc gatcgagctt gcgcgtgcgg cccgtgcgcc ggtgcgggtc gtacttggtc
    11761 gccacgagga gctggcggtc gcgggctatc gcccgccggc tgagctcgac ctcgccctac
    11821 tcgcgagccg gtcggggggc ctgcgcgcgc ttagcgtgcg tgcacacgcc gactccgggg
    11881 tggcggttgg ctcctcgatc gccgccctgt gccgcttcat ctaccccgcg ccggcgatcg
    11941 agctgctcga ccacgatgtc gtgaaccaca tgcctccggg caagcccttc cgcggtcccg
    12001 gagcgccagt cgcggcttgg gcgctcgagc aggccgtcga cgaggcggcc caccgcctgg
    12061 gcgaggatcc gctcgcgctg cgccgtcgtc tggatcccga tccgctgcgg cagaggctgt
    12121 acgagcgggc gcagggcctc gatgtgtggc gcggacgatc ccagacgggg ttgcagagcg
    12181 gccgcttccg gcgcggggtc ggcgtcgccg cggggaactg ggtctacgtg taccagctcg
    12241 actgcacggt cgaggtcggc atcgagggcg gtcgcctgtt cgtggccacc gctacgcagg
    12301 acatggggac gggaagccgc agcgtgctcg cagacgtggt tggtcgagcg ttcgggctcg
    12361 agtccgcgga cgtcgaggtg aggatcggcg actcgcgcca gctgcgcggg ccgacgtccg
    12421 gagggagccg aacgacggcg accatcgtcc cggcggcgct cgaggcggtc gcgcgcctac
    12481 gcgcccagat cgtgcaggct ctcgggctac gggacgcgcg ggaggacgac acggggatcc
    12541 gacacgcggc cggctcgatt tcctggaagg aggcgatcgc gtccgcaccg gacgtcacgg
    12601 ccgtggcgga ccggcccggc gacgatcaca cggcctcgaa cgccgccggg ccgctggccg
    12661 gagcgggtcc ggccggggcc gtgatgcgct gggcgatgcg caggtcgatg actctcgagg
    12721 tggggcgcgg ctccacgggc gcggtgcacg tgagcgagat cgaggtcgac acgatgctcg
    12781 ggaaggtgcg ggtacttaga gtggccgccg gtgtcgcggc cggtcggatc gccgcgcccg
    12841 ccctcgcccg gagccagtgc tacggcggcg tcattcaagg gatcggctac gggctgtacg
    12901 agcaacggca ggccgacacc cgcgacggtc tcgtgctgac ggccgggctc gaggactacc
    12961 gcatccccgg catcggtgac gtgcccgaaa tcgacatcca tttcatggag gacggcttcg
    13021 agcacgtacc cggcggtggc gtgggcctgg gcgagctctc gaccatcgcc gttgccgcgt
    13081 cggtcggcaa cgccgtctac aacgcgacgg ggtggcgccc gcgcgagctc ccgatccggc
    13141 ctgaccggct tgtcgccgga ctgtcatgag cgcgtcgccg ccgacgatcg agggcgcgct
    13201 cgcggccacc ggtgagtacc gggccggcgg caccgacctt cagcaacgcc tccgcagcgg
    13261 cgtctcgcac gggccggtca tcgatcttgc gggcctaccc ggcctcgacg cgatcgagtg
    13321 ggatgagcag ggccgagcga cgatcggcgc gctcgcgccg atcgcggcac tcgcgactga
    13381 tccccgcgtg ctcgcctcgt atcccggcct cgcgctttca gcgggtgcgc tcgcgacgcc
    13441 gcagatccgt gccgcgggca cgctcggcgg caacctgctg cagcgcaatc gctgctggta
    13501 cttccagaac ccgggcgtga actgcctcaa gaagggcggc gacgactgcc ccgcgcgatc
    13561 cggcaaccat ctctacggcg tctgcttcga cctcggcccc tgcgtcgccc cacacccctc
    13621 gacgctcggc atggctctcc tcgcctatgg ggcggaggtg gacgtggccg gcggcggtac
    13681 ccgctcggtt gcggcgctat acggcgatgg cagcgatggc cgccacgacc accagcttcg
    13741 cgagcacgag ctgctgaccg ccgtgctgat gcccgaaccg ctcgagggcg agcgcgcggc
    13801 gtacttccgg gcgatcagcc gcgccctggc cgagtggccg ctcgtggagg ccgtggtgcg
    13861 gctcgttgtt gctgacggca cggtccgctt cgcccgagtg gccgtgggtg gcgtcgctcc
    13921 ggtgccgctt cgcttggagt cggtcgagga agcactgctc aaccggccgg cctcgcccga
    13981 gaccttcgag gaggcggtct catcggcgac ccagggtgcg cgcccgctgc cgatgaccag
    14041 gtacaagctc gagctcctcc caggaacggt gcgcgagacg ctggagcgcg cggtcgccgc
    14101 cggcccctga cgcgctgcgt accgtcaatc agcgtcaccg gtgcctgccc ccgaagccgc
    14161 cgagggagac tcattcggcc cctgtcagcg agccgcgcac gcgcgcgtct ggaaattgcg
    14221 gcagccgtgc ggaaggcccg ccgatgccat ctgatcgccg acatagactc gccgttcgat
    14281 gtcgccgagc ccggccgtac acctgcatgt ccacagcgag tactcgctgc tcgacggcgc
    14341 ctgcaacata gagaacctcg cggccagggc ggccgagtac gggcagccgg cgctcgggct
    14401 caccgaccac ggcgtgatga acggcgcggt ggagttctac aaggcatgca agaagcacgg
    14461 cgtgaagccg atcatcggct tcgaggccta cctggtcgac gacctcaaga gcgacgcggt
    14521 caagtacgag cgcaaccacc tcacgctgct ggcctcgtca gccgagggct tccgcaacct
    14581 ggtgaagctg acctccgccg gcttcctcga gggctacaag cggggcaagg caaacgtcga
    14641 tctcgacctg ctgtcgcgcc attccaagga cgtgatcgcg ctcacgggct gcctccagtc
    14701 gcgcttctgc cgccgcctgg tcgaggacaa cccggccgag gcgcgcgagc acgtcgacga
    14761 tctgatgcgg atcttcgggc ccgagaacgt ctacttcgag gtacagaaga acgggctggc
    14821 agagcaggag aaggccaacg agggcatcgt gcgggcagcg cgcgagttcg gccggccgct
    14881 cgtcggcacc gccgacgtcc actacctgcg gcgcgaggac tacgaccacc acaaggcgct
    14941 cttgtgcgtg cagacgaagt cgacgctgct cgagccgaag ctcagcttcg acaccaacga
    15001 gttctatttg aagagctcgg aggagatggc tgcctcgttc ggggagtggc ccgaggcgat
    15061 cgcgtccacg ctcgagatcg cggagcgctg cgacgtcgag ctcgagctcg gaaagatgct
    15121 gattccgcgg tatcccacgc cggacggctc ggacgagggc gtgtacctgc gccggctcgc
    15181 cgaggaggga ctgcggcgcc gctacggcga cccggtgccg gcggaggcga cggagcggct
    15241 caacatggag ctcgaggtca tcgatcggat gggcttcaac gcttacttcc tgatcgtctg
    15301 ggactttgtg aagttcgcga aggacaacgg catcgcggtc ggtcccgggc gtggctcggc
    15361 ggccggctcg ctcgtgtcct acacgctgaa catcaccgac gtcgatccgc tccgctacga
    15421 ccttctgttc gagcgcttcc tgaacgccga gcgcgtgtcg atgccggaca tcgacatcga
    15481 cttctcggtg aaggggcgcg accgcgtgat ccggtacgtg accgagaagt acggcaccga
    15541 gtcggtggcg cagatcatca ccttcggccg catgttcccg cgcgcggcca cgcgcgacgc
    15601 ggcgcgcttg ctcggctacg actacggcgc gggcgaccgg ctggcgaagc tgatcccgga
    15661 cccgatcatg ggccgaccac cgtcgttcga cgactgcctg aagccggggg aggagctcgc
    15721 gaaggcgtac gcagaggacc cgcaggcgaa ggcgatcatc gacgtggcgc gcgggctcga
    15781 aggcacggtg cgaaacaact cgatccacgc ggcggcggtc gtgatcgcgg accgcccgct
    15841 caccgacatc gtgccgctcc agctggcgga ggaccgcggt gtggtcgacg aggacggcaa
    15901 ccgctcgtac cgcacggtca cgcagtactc gatgaagccg atcgaggaga tcggcctgct
    15961 gaaaatggac ttcctcgggc tgcgcaacct cgacgtgatc gagtccgccc tcgacatcat
    16021 cgagcgctcg ctcggcgagc ggcccgatat gaccacgctg ccgctcgacg acgcgaagac
    16081 gtacgagatg ctcgctcgcg gcgactcgac cggcgtgttc cagttcgagt cggagggcat
    16141 gcgcgaggcg ctgaagaagg tgcggccgac ggagttcgag gacctggtcg cgctcaatgc
    16201 cctctaccgc ccgggcgcaa tgcgtcacat cgacacgtac gcgcgcaaca agcgcaaccc
    16261 ggaggcagtc acgtacatcg acgaccgcct gcgcccgata accgagtcga cctactcggt
    16321 gatcctctac caagagcagt cgatgcagat cgcgaagggg atcgccgggt tctcgggtcc
    16381 cgaggccgat gacctgcgca aggcgatcgg caagaaggac cgcgcgaaga tggcgtcgct
    16441 gaagcagcgc ttcgtggagg gcgcgcgcgc gacgggcacg tccgaggcag tgatcgaaca
    16501 gctgtggcag accaacgagg cggcggccga ctactccttc aaccggtcgc acgcggcctg
    16561 ctacgccctg atcgggtatc gcaccgcctg gctgaaggcg aactacccgg ccgagtacat
    16621 ggccgcgctg atctcgtcgg tgatgtccac gaaggacaag gtgccgttct tcgtctcccg
    16681 ctgcgaggag atggggatcg aggtgctgcc gcccgacgtg aacgagtcgg ggcacgactt
    16741 cgtggtcgtc gagggtcaca tccgcttcgg gctcgacgcc gtgaagaacg tcggctcggc
    16801 tgccgtcgac gcgatcctcg aggcgcgcga gcagggcggc ccgttcgagt cgatctggga
    16861 tttctgcgag cgcgtcgact gtcgcgcggt caacaagaag gcgatcgagg cgctgatcaa
    16921 gtgtggcgcg ctcgactcga ccggcgcttc tcggcgaggg atgctcgaga tcatgcccca
    16981 ggcgcaggcg tccggacaga aggctcagga ggacgcgctg cttgggcagg gctcgatctt
    17041 cgacttcgac gagccgtcgg cgggcggcgg cgggcccgcg cccttccagg cggcgcggcc
    17101 ggtgccgaac ctgtccgacg agcgctccga cctcaacctg atggagaagg agactctcgg
    17161 gctattcctc tcgagccatc cgctcaagga ggtgcgcgcc gcgctgcgcg caaaggtcga
    17221 gtgctcgata gccgagctcg ccgacaaaaa ggacggcgat tgggtcacgg tcggcggcat
    17281 gatctccgag gcgaagcgga tccgcaccaa gaagggcgat ccgatgatgt tcgccacgct
    17341 cgacgacctc gagggccagg tcgagatcct ggtcttcaac tcggcgtact ccgagaacgc
    17401 ggagaagatc gacgtcgact cggtggtcgt cgtgcgcggg cgcgtggacc acaaggagca
    17461 gggcgagacc aagctcgtag tgcagtcggt ggagctgttc gaggcaacgc ccgcggagat
    17521 cgcgaaggcg caggaggacg ccgccaacat ggtgccgcct ccgccgaagc ggataacgct
    17581 cgaggtccca ggctcggtgt ccgagagctt cctcgacgag ctgctcgagg tcgtgagcca
    17641 tcaccgcggc gagtacgagc tgtgcctgaa gatcgggccc cgcgcgctgt tgctcggccc
    17701 cgaccaccgg gtctcgggga cgagttcgtg ccgaagcgag ctcgagaacc tcgtagcgct
    17761 ggcgcaagcg gcgtagaggg gcgcgtgggc ggcgttcggc tcgtcccact cgggcgagag
    17821 tcgacaacat cgcttccgaa cgcgtcgcgg aacggtgcgg ctacgtgcgc gagggcgtga
    17881 tgcgctcgct gtacctgaag ggcgacatcc gcgtcgacgc gtccctctgg tcacgcctgc
    17941 cgaccgacgc gtgaacggcg cgtctcgctc tgcgagtctt agcgacggca tccagggcgc
    18001 aggaagggag atcaatgacg agtcactcct caacacagac gccggggaca aatgggcgtc
    18061 gccgctcgcg ggtcgatcag ttggttcgct ccacgcttcc tcagaagctt ccaccgccgg
    18121 caaatccggc actgctcaag cacgcggagg agcgcgccga caatgcccaa aaccggcttg
    18181 cggaccggat cacggccttc tcgggctcaa tgctgttcgt gtacctgcac atcctctggt
    18241 tcgcatgctg gatcggcttc ggcgtcgaga aatatccatt cggcctgctg acgatgatcg
    18301 tgtcgctcga ggcgattttc ctctcgacct tcgtgttgat cagccagaac cgagcggatg
    18361 cgaagcggca ggtgcaggcc aacgagcagt ggcgcacggt gcaggaggag gatcgccaaa
    18421 acgacgagct cttgagcctc tcgaaggaga tcctgaagct cacgcaggag gtccactcgc
    18481 gccaaagttg aggacgtgac gtagcggccc gagcggacgg aaatttggct ctagggctgg
    18541 cgcgccaggc tcgctggcat gggcgagcgg gtctacactt tccaaatgga cggcgccgta
    18601 caggaacgca tcgcgcacga gtgcaggcag tgctgcgcgt tctgtgatcg cctggtccag
    18661 cccgcgggct gcatcgaggc gaactgccca tacctctacg tctacgacga cgagcagacc
    18721 ggtggccgct acatgggctg cctcaacaag gtcttccgcg gcgagatcga cgtcgagctc
    18781 ttccgcgagg cggagaagac gcggcacggc ttcggtggcg tgaagatgtc gggccgtccg
    18841 acatcgttct gccgcttcac cgtcgagcgc gcctatcacg gcgagggcga ggcgttcgac
    18901 tgcgtgaacc cggagttctt cgagggccct gagccagtcg acggtctcca ttcgttcgac
    18961 ttgcgcgacc gcctctaacg ctccgaaata ccgggcgccc cacttgcgtt ccgtcggcct
    19021 catggggtag gttcgctccc cgtgatgggt cggcacgagg ccggcccggg tcctggcccg
    19081 gaggttctgc accatgctgc atgcgtccgc ccgcgcgtgc cgcgcgccac ggatggttgc
    19141 ccgcttcgtc tcgctggctg ccgtcgcgat gctcgtcacc gcggccgcac cggctgtcgc
    19201 gcgcgccgcg gactacgtcg ccatgggcga ctcgtactcc tcgggcacgg gcacgcgtga
    19261 gtactacgaa ggctgcgagc ggagcgtcta cgcgtacccg tacctgatca agggctcatt
    19321 cggcagcagc ttctcgttcg tcgcgtgcgg cggcgcgaag actcaggacg tgctgaacaa
    19381 ccaggtctcg gcgctgagct ccgggacgga gttcgcgacg atctcgatcg gcggcaacga
    19441 cgccggcttc tcgagcgtga tcaccaagtg cgcgcagccg atcgtgagct gcgacggtga
    19501 catcaacaac gcgcagtcgt tcattcagaa cacgctgccc gggcggctcg acaacgtcta
    19561 caaccagatc cgcagccgcg cgccgaacgc ggtggtcggc gtggtcgggt acccgcgcct
    19621 cttcaacggc gaggactgca acctgggcac cttcttcagc tcctcggaga tgacgcgcct
    19681 caatcagacg gccgacctgc tcgcgaatgt cacgcgggcg cgcgcgcagg cgcacggctt
    19741 cacgttcgtc gacgcgaggc ctgcgttcat cggtcacgcg gtctgcgaca acgtggagtg
    19801 gctgaacggc ctgtcgaacc ccacgagcga gtcgtaccac ccgaatcgaa ccggccacag
    19861 ctcgggctac gcgagcatcg tccgggccgc gctgctggcg gcgcccgacc cgaatgctcc
    19921 tgtcggcggc aacggccgca tcgccttcgt gagcacgcgc ggcggcaaca gcgacgtctg
    19981 ggtgatgaac aacaacggcc agtttccgat gaacctcacc aacgcacccg gcgaggacgt
    20041 cgatccggtc tggtccgctg acggcacgca gatcgccttt gcgagcaacc gcgacggcga
    20101 caacgagatc tacgtgatga acggcgacgg cacgggcgtc acgaagctca cgaacaacac
    20161 gaacgacgac cgcgacccga cctggtcccc gaacggcgac tacatcgcgt tcaagagcga
    20221 tcgcaccggc aacaacgaga tcttccgcat gacgtcatcg ggcggatcgc agaccaacct
    20281 cacgaacaac tccgcgtccg acttctcacc ggattggtca ccggacggcg ccgaggtcgt
    20341 gttccagcgc ttcacgaacg gcagcgcgac cggcaacggc aacgaggtct tcaagatgaa
    20401 cgccgacggc cagggccaga cgaacctgac caacaacgcg gcatcgatca acgacgggtc
    20461 gccggcgtgg tctcccgacg gcaccacgct cgccttccat agcaaccgcg acggcgactt
    20521 cgaggtcttc acgatgcccg caatcggtgg ctcggccaca cagcgcacga gcaacaccgc
    20581 gagcgatggg gaaccggcgt gggcgccgaa cggcacccaa ctcgccttcc agagcaaccg
    20641 cgacggcaac aacaacgaca tcttcacgat gacgtcgagc ggcgggtcgc agacgaaccg
    20701 ctcctcgaac cctgcgacgg acgtgagccc gagctggcag gcggacagca ctccgccgct
    20761 gacgtcgatc accgtcgggc cgaacggacc gacgaacact tcgacgccgc agttcgagtt
    20821 cacctcctcg gagctcggct cgaatcttca gtgccgcctc gacaacggca gcttccagac
    20881 gtgtccgtcg ccgttcacga ctgcgtcgct ggcggatggg ccgcacacgt tctcggcgcg
    20941 ttccgtcgac ccggcgggca acgtcgaccc gaacccggtc gtgcggacgt tcctggtcga
    21001 cacgaaggcg ccgagcctct cggtcgagtg ccccgactcg atgcttctca acgcacctgg
    21061 ctcggcgatc gtgacggcgt cggacgccgg ctcggggctg cctccgggcg aggacccgag
    21121 cggcagctat ccgctcgaca cgtcgcagcc cggcagccag agcttccgca tcgacgcgtt
    21181 cgacctcgcc ggcaaccagg cgagcgccga gtgtgactac gaggtgctct accccgcgcc
    21241 gggcacgccg gacgtcacca gcggtgggaa cccgaacgcc ggcgcgttca cgctcagctg
    21301 ggtcccggcc gcgccgcccg agtacgggct gcattacacg ctcgagcgcc gggacgccga
    21361 cgacgacgcc tggcagcagg cggcgagcgg gatcgacggc acggactact cgttcactgc
    21421 gggcgattcc gcggacgagg ggacctggag ctatcgcgtg aagggttccg acggcgatca
    21481 cgagacgccc tggtccgagg tgtcggcgtc cgtcaaggtc gaccagacgg cgccggcaaa
    21541 gccggccatc gtgccggacc gcgcgcccga ctacgccggc gacggcggct ggtaccgcga
    21601 ctcggtgctc gtctcgaccg aggatcgcgg ggatccgacg ctgcgcgatg gcagcgaccc
    21661 gaccggcgtc gacgcgagca cggtcgccgg gccggagctg ctcgacgcga gcggcacggt
    21721 tcatcgcacc gtgagcgatc gcgtgggcaa cacctcggac gaggcgacgg aggacttcca
    21781 ggtggacacc gagaagccct cgctccagct cgactgcccg gaggacgtag tcctaggcgg
    21841 tgaggcgtcg gtggaggtga ctgcagggga cagccagtca ggcctggcga gcgacccgag
    21901 cggcgccgtc gaagtcgaca cgagccacgt gggcacgcag accatcgagc gcaccgcgac
    21961 cgacaacgtc ggtcacgagc gcacgaagag ctgccacgtg ctcgtccggt acgactacgg
    22021 cggcctgctg cagccggtga accgcgacgg ctcgagcgtg ttcaagctcg ggagcacgat
    22081 cccgctcaag ctgcggctgt ccgaccacgc cggcaactcg gtcgccggcg cccacgtgga
    22141 agtgcagctg gagcggacgt ccacgtcggt cgacggcacc gagttcgagg aggtcgtcga
    22201 cgccacgcct acgatcggga agacgttcga gtacagcgat ggttcggagg tctaccgctt
    22261 caacctctcg acgaagccgc tgagcgcggg cacgtggcgc atacgcatct cactcgacga
    22321 cggcaccgtg caccgcacgc agatctccct ccggtaggcc gctagctcga cac
//
LOCUS       DASVXS010000212         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_554265, whole genome shotgun sequence.
ACCESSION   DASVXS010000212 DASVXS010000000
VERSION     DASVXS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554265"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VF752_11935"
     CDS             complement(<1..731)
                     /locus_tag="VF752_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8856297.1"
                     /translation="MAAAIETAGLVKRYGDVEALAGVDLRAEEGTVLGLLGPNGAGKT
                     TAVRILSTLLKPDAGSARVAGLDVVKQAGELRHRIGLAGQYAAVDESLTGAENLQLFA
                     RLYGMSRRDARRRAAELLDRFDLSDAADRLVGTYSGGMRRRLDLAGALVARPPILFLD
                     EPTTGLDPRSRLSLWKTITDRVAQGTTVLLTTQYLEEADRLADQIAVIDHGRVIAEGT
                     ANDLKDRVGGMRVVVHVGEGTDSAHA"
     gene            complement(776..1447)
                     /locus_tag="VF752_11940"
     CDS             complement(776..1447)
                     /locus_tag="VF752_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8856298.1"
                     /translation="MCRARERVPNRQMQRAEPNDATPFLPDRLNLEALREASAGCRGC
                     HLWRKATQTVFGEGRKGSRVMFVGEQPGDREDREGRPFVGPAGRELAQALDQVGIARD
                     EVYITNVVKHFKFEERGRRRIHQTPKRFEIDACMPWLNAELEVVKPEALVLLGATAGK
                     ALFGSKFRIREARGRPLESDLAPFVTATIHPSAILRAPDDESRLLEREAFTEDLAAVA
                     RAMVG"
BASE COUNT          249 a          611 c          583 g          255 t
ORIGIN      
        1 gcgtgcgccg agtcggttcc ctcgcccacg tgaacgacga cgcgcatgcc gccgacgcgg
       61 tccttcaggt cgttggcggt gccctcggcg atcacgcggc cgtggtcgat caccgcgatc
      121 tgatccgcga ggcggtcagc ctcctccagg tactgagttg tgagcaggac cgtggtgccc
      181 tgggcgaccc ggtcggtgat cgtcttccac aggctcagac ggctgcgcgg atccagcccc
      241 gttgtgggct cgtcgaggaa gaggatcggc ggcctcgcga cgagcgcgcc ggcgaggtcg
      301 aggcggcggc gcatgccgcc cgagtacgtg ccaacgaggc ggtcggcagc atcgctcagg
      361 tcgaagcggt cgagcagctc ggcggcgcgg cggcgcgcgt cgcggcgcga catcccgtag
      421 aggcgcgcga acagctgaag gttctcggct ccggtgaggc tctcgtcgac cgccgcgtac
      481 tgccctgcca ggccgatccg gtggcgcagc tcgccggcct gcttcaccac gtcgaggccc
      541 gcgacgcggg cgctgccggc gtcgggcttc agcagcgtgg agaggatccg taccgcggtg
      601 gtcttgccag cgccattggg cccgagcaga ccgagcacgg tcccctcctc ggcgcgcagg
      661 tccaccccgg ccagcgcttc cacgtcgccg tatcgtttga cgagtcccgc tgtctcgatc
      721 gcggcggcca tcccgccagg cacgctagta aacgcgcccg cgagcgcagg tacggctaac
      781 cgaccattgc ccgcgccacc gcggccaggt cctccgtgaa cgcctcgcgc tcgagcagcc
      841 gcgactcatc gtcaggcgca cgcaggatcg ccgatgggtg gatggtggcg gtcacgaacg
      901 gggcgaggtc cgactcgagc ggcctcccgc gcgcctcgcg gatgcggaac ttcgagccga
      961 acagcgcctt ccccgcggtg gcgccgagca gcacgagcgc ctcgggcttc accacctcga
     1021 gctcggcgtt gagccacggc atgcaggcgt cgatttcgaa gcgcttgggc gtctggtgga
     1081 tgcgccggcg gccgcgctcc tcgaacttga agtgcttcac cacgttcgtg atgtagacct
     1141 cgtcgcgcgc gatcccgacc tgatcgagag cctgcgccag ctcgcgcccc gcgggaccca
     1201 cgaacggccg cccctcgcga tcctcgcgat ctccaggctg ctcgcccacg aacatcaccc
     1261 gcgagccctt gcgaccctcg ccgaacacgg tctgcgtcgc cttgcgccag aggtggcagc
     1321 cgcggcaccc cgcggaggcc tccctcagcg cttcgaggtt gagcctgtcg ggcaggaacg
     1381 gcgtcgcgtc attgggctcg gcgcgctgca tctgccggtt cggtacccgc tcccgcgcgc
     1441 ggcatatcgc ggagcgtcgt cacttggggg ccactatggc ccctgatcga cgacgttcgg
     1501 gaatgagggc ggccgtcatc actcacatcc cactatgagg aggaaatgac gacgcttggc
     1561 acttacgggg ggagcgccgt cacttagggg ccactatggc ccctgatcga ccacaccgag
     1621 ggagccaggg gcgagcgtcg tcactcaggg tgaatagttc accgcgagcg acgacagccg
     1681 ctcaacccca caccgggc
//
LOCUS       DASVXS010000213         9367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_594957, whole genome shotgun sequence.
ACCESSION   DASVXS010000213 DASVXS010000000
VERSION     DASVXS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594957"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VF752_11945"
     CDS             <1..1452
                     /locus_tag="VF752_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8856299.1"
                     /translation="QLPARGRQLMRRRGSASIAANPVLIGAATTLVVIVAVFLAYNAN
                     NGLPFVPTYDLKVDLPNAANLVKGNDVRIGGTRVGIVSTITPVRRSDGTSFARVEIKL
                     EKRVDPLPQDSTVIVRPRSALGLKFVEITPGVSSAGYANGATIPLAQAQPKPVEIDQV
                     FNMFNARTRRGSQQSIRGFGDALIGRGADINLAIEDFKPLLKHLEPVARNISAKQTQF
                     DRFFPALERAAAEVAPVAQQQGELFANLDTTFTALASVARPFLQETISKSPPTEQTAI
                     EEFPKQRPFIRNSAAFFHELQPGVAVLPSTAPVLASALEAGIPALRRSPPFNKDLADS
                     FEALQKFATNPVVPAGIDRLDKTMKSLNPTLKFLTPVQTTCNYVSVLLRNAQSLLSEG
                     DGHGTWQRFVIITAPIGPNSEGGPSSAPANGPTVDNHLHANVYPNTASPGQTKECEAG
                     NEVYLVGKTLIGNVPGSQGTVTDLQPQKSGSGG"
     gene            1449..2903
                     /locus_tag="VF752_11950"
     CDS             1449..2903
                     /locus_tag="VF752_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8856300.1"
                     /translation="MRRGHKQGLSPFQAGLIATVIIAIVSFLVFSKDIPFTNPYELKA
                     VFNNATTLAPNSPVRIAGVEVGKVKSVDAMTDSNGHQTNASVVTMQINSNGLPIHRDA
                     VLKIRPRIFLEGNFFVQIEPGTPSAPTVHSGETIPIAQTKAPVQIDQVLTTLQSDPRT
                     NLQKLLFGYGNALNGQPLPGEDADQDPAVRGLTAGQALNKSLKYSPDALRGTALLNQG
                     LLGIEPQDVSKLVRGGRKVAAALNANEQQLQDFITNFNRTAAAFATESGNLRQTIHLL
                     PQVLEQAQPTLVKLEQSFGPTKAFAHDLIPGVKETPATIEAAFPWIAQTRKLVAPSEL
                     GGLLNDLQPAVRDLASATDATIKFLPQADLLARCTTHNILPTGDKPIQDQFATGVANY
                     KEFWQSMVGLAGESQNFDGNGPYTRFQTGGGANPVKTQNVVGANFGSLFGNAIRPILG
                     TSPTQPSKTPPIRTDVACYKNQVPNLAPKVGPGP"
     gene            2900..4261
                     /locus_tag="VF752_11955"
     CDS             2900..4261
                     /locus_tag="VF752_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8856301.1"
                     /translation="MKTAIRKHMRDFVAIVAVFLLALGVAGYVLSHQRFYLPAWVPVV
                     GTDFYTIHAEFQTAQAVVPGQGQTVNIAGVPIGEIGNVELKNGVANVELKIKRKYAPL
                     YRDAHMLLRPKTGLKDMFISTTRGHPSAGKVPEGGTIPVANTLPDVNLDEILANLDTD
                     TRSYLAVLLDAGGEAFGAKGYNAALRQTFKRFLPTNRDVAKFTSLVSQRRRNVRRVVH
                     NFQLLTTELAKRDKQLTQLVDSSNANFQAIGSQDQSIEEALRLLPPTLTTAQSALTKA
                     NRLATDLGPALEALRPGARALAPALRASRPFLRGTTPVIENQLRPFAREAQPPVEQLR
                     RAASGLSAATPGLVSTAQFLNKVVNALAYNPSGPEEGFLYWLAWGNHIGATIFGTQDA
                     HGPIRRGIFLTDCTSLSVLQRIGASNPNLQVLIDLLNAPSPQQACGTAAPLVGGAAKA
                     GGK"
     gene            4261..5790
                     /locus_tag="VF752_11960"
     CDS             4261..5790
                     /locus_tag="VF752_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8856302.1"
                     /translation="MQKQAPSIGRVLVMVFFALSCVGILLYLWLVFGGATPLRAKGYI
                     VHVDFPEATTLANEADVRISGVSVGKVKTKKLVIVNGKPKPVTDVKLEIDRQYAPIPS
                     DTRAILRQKSLLGETYVELTPGSNSAPKVADGGQLAFGNVSQTVELDEIFRTFDPKTR
                     AAFQTWMEQQGQAFRGRGQAVNDALGNLTPFAENTDKVLQVLDSQAADTRRLIRNTGV
                     VFDALTERNGQLRDLIVNSNRVFHTTASRDQKLADAFRAFPSFLDESRATLTRVSTFA
                     KTTNPLITQLRPAARELSPTLIALEGLAPDLRNLFVNLGPLITVSKKGLPALTHFLNI
                     TPPLLAQLDPWLRQLNPILDWLGLYKREIAAFFANDSAATQATGTRTATATPLHYLRT
                     MNPVNPEVLAAYPHRISTNRSNPYIEPGGYSKLPSGLEVFDPALCTSNPVPQLSRDTS
                     NLDPDMAAMFQTRTFDLIATFVYGGQPPESVPAPPCKAQAPLGRLLGQPGRYPHVTAQ
                     P"
     gene            5840..8548
                     /locus_tag="VF752_11965"
     CDS             5840..8548
                     /locus_tag="VF752_11965"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8856303.1"
                     /translation="MLKRESFARIVGAAARRPMLVLAIVGALAIAGAVLALRLEPDAG
                     TGTLVSHNSKSFQATERFKKTFGDDAVIVLVQGNLQKTVLTSDLGRLLRLEGCLSGNV
                     PKAGLKDLPAECTWFAQHKPAKVVYGPGTFVNTAAARLSEGFTQRQQQEQQAADAAAK
                     IARQKAAKQGLSAAGQKKAADDARAAVEQQFKLSILKLVLRYGITSVPALDNPEFVSQ
                     LVFDPSRGVGQPKARFAYLFPSSNSAVVQVRFKPGLSDSERSAAIAKVRQAARASQFK
                     LQNGGRYVVTGVPVVVDGLATAVKHSIFILLAAAIVVMAGTLALVFRSRLRLLPLAVA
                     LVAAALTFGAVSLAGGSLTMASIAVLPVLIGLSVDYAIQFQARYDEARLEGRPPEEAA
                     PAAAAAGGPTIATAGLATAVGFLVLLLSPVPMVRGFGAMLVLGIVIAFACALTAGFAA
                     LVRFQDGSPGALPGLRDRASAAGERIADTRAWSWLAHHGHRGRELALDRGIRIVATSI
                     RRPRAVLGAALAIAVLGWAADTQTHVISDVRELVPQNLQALKDVNTLQKETGVSGEID
                     VVLTGDKLATPQVIDWMTRFQSGVLQAHGWTTGATCAQRKNPPEICPALSLPDLFRSG
                     GVTSQAQIDGLLQAVPRYFSQAVISPDQKTATLAFGIRLMPLDRQKQVIDDIERRLDP
                     PAGVQASVAGLPVLAAEANSALSSTARRALTLLAGLVAVFLVLLIVRRRLRLATIPLI
                     PIALATGWSALVLFLLRIPLNPMSATMGALVIAISTEFSVLLSSRYEEERRAGAAPAR
                     ALELTYASTGAAVLASGVTAIAGFAALIASDIRMLRDFGIVTVVDLTLALLGVLVVLP
                     AALVWAEQHGKFTLSDIDPRRGVRALRDAVRRRRARGGTALPARRA"
     gene            8545..9291
                     /locus_tag="VF752_11970"
     CDS             8545..9291
                     /locus_tag="VF752_11970"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8856304.1"
                     /translation="MSEDRFGDLGPSGHDEGRSAAERLADMDERQLREERERRPPEED
                     RRPRGRYSWVIGIAFVIVIVVAGLNSIPHSGEGVKGPKPGEPLPKFAAPLATGTSNAD
                     ANVKQKASDSDQLGKKPACDVRGPGIFNLCDAENKPLVLTFIVTRGANCKPQLDRVEQ
                     VRRQFPGVGFVGVVSGDDRTKVAKIVRERGLRYPVAVDHDGELVNVYGIGVCPATVFA
                     KPGGKVLVTKLGRLTDQQLRAQVQALLKAS"
BASE COUNT         1487 a         3367 c         3189 g         1324 t
ORIGIN      
        1 caactacctg ctcggggacg gcaactgatg cgccggcgcg gctccgcatc gattgctgcc
       61 aacccggtgc tgatcggcgc cgcgaccacg ctcgtcgtca tcgtcgccgt gttcctggcc
      121 tacaacgcga acaacggcct gccgttcgtg cccacgtacg acctgaaggt cgacctaccg
      181 aacgccgcca acctggtgaa gggcaacgac gtccgcatcg gcggcacccg cgtgggcatc
      241 gtcagcacga tcacgccggt gcgccgcagc gacggcacga gcttcgcgcg cgtcgagatc
      301 aagctcgaga agcgcgtcga cccgctgccg caggactcga cggtgatcgt gcggccgcgc
      361 tccgcgctcg ggctcaagtt cgtggagatc acccccggcg tgtcgagcgc cgggtacgcg
      421 aacggcgcca cgatcccgct cgcgcaggcc cagccgaagc cggtcgagat cgaccaggtc
      481 ttcaacatgt tcaacgcccg cacgcggcgc ggctcccagc agagcatccg cggcttcggc
      541 gacgcgctca tcggccgcgg cgccgacatc aacctcgcga tagaggactt caagccgctg
      601 ctgaagcacc tcgagcccgt ggctcgcaac atctcggcga agcagacgca gttcgaccgc
      661 ttcttcccgg cgctcgagag ggccgccgcg gaggtcgcgc cggtcgccca gcagcagggc
      721 gagctgttcg ccaacctcga cacaacgttc acggcgctcg cgagcgtcgc gcggccgttc
      781 ctccaggaga cgatctctaa gtcgccgccc accgagcaga cggcgatcga ggagttcccg
      841 aagcagcggc cgttcatccg caatagcgcc gccttcttcc acgagctgca gccgggcgtg
      901 gccgtgctgc ccagcaccgc gccggtactc gccagcgcgc tcgaggcggg catccccgcg
      961 ctccgccgct cgccgccgtt caacaaggac ctggcggaca gcttcgaggc gctgcagaag
     1021 ttcgcgacca accccgtggt cccggcgggc atcgaccggc tcgacaagac gatgaagtcg
     1081 ctgaacccga cgctgaagtt cctcacgccg gtccagacga cctgcaacta cgtctccgtg
     1141 ctgctgcgca acgcccagag cctgctctcg gagggcgacg gccacggcac ctggcagcgc
     1201 ttcgtgatca tcaccgcccc gatcgggccg aacagcgagg gtggcccgtc tagcgcgccg
     1261 gcgaacggcc cgaccgtgga caaccaccta cacgcgaacg tctacccgaa cacggcgtcg
     1321 ccggggcaga ccaaggagtg cgaggcgggc aacgaggtct acctggtcgg caagaccctg
     1381 atcggcaacg tgccgggcag ccagggcacg gtcaccgacc tgcagcccca gaagagcggg
     1441 agcggcggat gaggcgcggc cacaagcagg gcctgtcgcc gttccaggcg gggctgatcg
     1501 cgacggtgat catcgcgatc gtcagcttcc tcgtgttctc gaaggacatc ccgttcacga
     1561 atccctacga gctgaaggcg gtgttcaaca acgccacgac cctggcgccg aactcgccgg
     1621 tccggatcgc gggcgtggag gtcggcaagg tcaagagtgt cgacgcgatg accgacagca
     1681 acggccatca gacgaacgcc tcggtcgtga cgatgcagat caacagcaac ggactgccga
     1741 tccaccgcga cgcggtgctg aagatccggc cgcgcatctt cctcgagggc aacttcttcg
     1801 tgcagatcga gcccggcacg ccgagcgcgc cgacggtgca ctcgggcgag acgatcccga
     1861 tcgcccagac caaggcgccg gtgcagatcg accaggtgct gaccacgctg cagtccgacc
     1921 cgcggacgaa cttgcagaag ctgctcttcg gctacggcaa cgcgttgaac ggccagccgc
     1981 tgccgggcga ggacgccgac caggatccgg ccgtgagagg gctgaccgcc gggcaggcgc
     2041 tcaacaagtc gctcaagtac tcgcccgacg cgctgcgcgg gaccgccctt ctgaaccaag
     2101 gcctcctcgg catcgagccg caggacgtgt cgaagctcgt ccgcggcggt cggaaggttg
     2161 ccgccgcgct gaacgcgaac gagcagcagc tgcaggactt catcaccaac ttcaaccgca
     2221 cggccgctgc cttcgccacc gaatccggca acctgcgcca gaccatccac ctgctgccgc
     2281 aggtgctcga gcaggctcag cccacgctgg tgaagctcga gcagtcgttc ggcccgacga
     2341 aggccttcgc gcacgacctg atccccggcg tgaaggagac gccggccacg atcgaggcgg
     2401 cgttcccgtg gatcgcgcag acgcggaagc tcgtggcgcc gagcgagctc ggcggcctgc
     2461 tcaatgacct ccagcccgcg gtgcgcgacc tcgcctccgc taccgacgcg acgatcaagt
     2521 tcctaccgca ggccgacctg ctcgcgcgct gcacgacgca caacatcctg ccgactggcg
     2581 acaagccgat ccaggatcag ttcgcgaccg gcgtcgcgaa ctacaaggag ttctggcaga
     2641 gcatggtcgg gctcgcgggc gagtcgcaga acttcgacgg caacggcccg tacacgcgct
     2701 tccagaccgg cggcggcgcc aaccccgtga agacccagaa cgtggtcggc gcgaacttcg
     2761 gcagcctctt cggcaacgcg atccgcccga tcctcggcac ctcgccgacg cagccgtcga
     2821 agacgccgcc gatccggacc gacgtcgcct gttacaagaa ccaggttccg aacctggcgc
     2881 cgaaggtggg gccgggaccg tgaagacggc gatccgcaag cacatgcgcg acttcgtcgc
     2941 aatcgtcgcc gtcttcctgc tcgcgctcgg cgtggcgggc tacgtgctct cgcaccagcg
     3001 cttctacctg ccggcgtggg tgccggtcgt gggcacggac ttctacacga tccacgccga
     3061 gttccagacc gcccaggcgg tcgtgcccgg tcagggccag acggtgaaca tcgccggcgt
     3121 gccgatcggc gagatcggca acgtggagct gaagaacggc gtcgcgaacg tcgagctgaa
     3181 gatcaagcgc aagtacgcgc cgctctaccg cgacgcgcac atgctgctgc gcccgaagac
     3241 gggcctgaag gacatgttca tctccaccac tcgcggccac ccctcggccg gcaaggtgcc
     3301 cgagggcggc accatcccgg tcgccaacac gctgcccgac gtcaacctcg acgagattct
     3361 cgcgaacctc gacacggaca cgcgctcgta cctggcggtg ctgctcgacg ccggcggcga
     3421 ggcgttcggg gcgaagggct acaacgcggc gctgcgccag acgttcaagc gcttcctgcc
     3481 cacgaaccgc gacgtcgcga agttcacgag cctcgtttcc cagcgacggc gcaacgtgcg
     3541 gcgggtggtc cacaacttcc agctgctcac caccgagctc gccaagcgcg acaagcagct
     3601 cacgcagctc gtggactcct cgaacgcgaa cttccaggcg atcggcagcc aggaccagag
     3661 catcgaggag gcgctgcggc tgctgccgcc cacgctgacc accgcccaaa gcgctctcac
     3721 gaaggcgaac cgcctggcca ccgacctcgg cccggcgctc gaggcgctgc gcccgggcgc
     3781 ccgcgcgctg gcgccggcgc tgcgcgcgag ccggccgttc ctgcgcggca ccacgccggt
     3841 gatcgagaac cagctgcggc cgttcgcgcg cgaggcgcag ccgcccgtgg agcagctgcg
     3901 gcgcgcggcg agcggtctgt ccgccgccac gccagggctc gtgagcaccg cgcagttcct
     3961 caacaaggtc gtgaacgcgc tcgcctacaa cccgagcggc cccgaggagg gcttcctcta
     4021 ctggctcgcc tggggcaacc acatcggcgc cacgatcttc ggcacgcagg acgcgcacgg
     4081 gccgatccgc cgcggcatct tcctcaccga ctgcaccagc ctcagcgtgc tccagcgcat
     4141 cggcgcgagc aacccgaacc tgcaggtgct gatcgacctg ctgaacgcgc cctcgccgca
     4201 gcaggcctgc ggcacggcgg cgccgctcgt gggcggcgcg gcgaaggcgg ggggcaagta
     4261 gtgcagaagc aggctccaag catcggccgc gtgctcgtga tggtgttctt cgccctctcc
     4321 tgcgtgggca ttctgctcta cctctggctc gtgttcggcg gcgccacgcc gctgcgcgcg
     4381 aagggctaca tcgtgcacgt cgacttcccc gaggcgacga cgctcgcgaa cgaggccgac
     4441 gtgcggatct cgggcgtgtc tgtcggcaag gtcaagacga agaagctcgt gatcgtgaac
     4501 ggcaagccga agccggtcac cgacgtgaag ctcgagatcg atcgccagta cgcgcccatc
     4561 ccgagcgaca cgagggcgat cctgcgccag aagtcgctgc tcggcgagac ctacgtggag
     4621 ctcactcccg gcagcaactc ggcgccgaag gtcgcggacg gtggccagct cgccttcggc
     4681 aacgtgtcac agacggtcga gctcgatgag atcttccgca cgttcgaccc gaagacgcgc
     4741 gcggcgttcc agacttggat ggagcagcag ggtcaggcgt tccgcgggcg cggtcaggcg
     4801 gtgaacgacg cgctcgggaa cctgacgccg ttcgcggaga acaccgacaa ggtgctgcag
     4861 gtgctcgaca gccaggcggc cgacacgcgc cgcctgatcc ggaacacggg cgtggtgttc
     4921 gacgcgctca cggagcgcaa cgggcagctg cgcgacctga tcgtgaactc gaaccgcgtg
     4981 ttccacacga ccgcctcgcg cgaccagaag ctcgccgacg cgttccgcgc gttcccgagc
     5041 ttcctcgacg agtcgcgggc cacgctcacg cgcgtgtcga ccttcgccaa gaccaccaac
     5101 ccgctcatca cccagctgcg ccccgcggcg cgcgagctgt cgcccacgct gatcgcgctg
     5161 gagggcctgg cgcccgacct gcgcaacctg ttcgtgaacc tcggtcccct gatcaccgtg
     5221 tcgaagaagg gtttgccggc gctcacgcac ttcctcaaca tcaccccgcc gctgctcgcg
     5281 cagctcgacc cgtggctgcg ccagctcaac ccgatcctcg actggctcgg gctctacaag
     5341 cgcgagatcg ccgcattctt cgcgaacgac tcggccgcca cgcaggccac gggaacgcgc
     5401 acggcgacgg ccacgccgct gcactacctg cgcaccatga acccggtgaa tccggaggtg
     5461 ctcgccgcgt atccgcaccg catctccacg aaccgctcga acccgtacat cgagccgggt
     5521 ggctactcga agctgccgag cggcctcgag gtgttcgacc ccgcgctctg cacgagcaac
     5581 ccggtgccgc agctgagccg cgacacgagc aacctcgacc ctgacatggc cgcgatgttc
     5641 cagacgcgca cgttcgatct gatcgcgacg ttcgtgtacg gcgggcagcc gccggagagc
     5701 gtgccggcgc cgccctgcaa ggcgcaggca ccgctcggtc gcctgctcgg ccagccgggc
     5761 cgctaccccc acgtgacggc gcagccgtag ggcgcgctta gcggcgttga tcgggtcccc
     5821 cgtcgcctaa gctgaagccg tgctgaagcg ggagtccttc gcacggatcg tcggggcggc
     5881 cgcgcgcagg cccatgctcg tgctggcgat tgttggggcg ctcgcgatag cgggtgccgt
     5941 cctcgcgctg cgcctcgagc cggatgccgg caccggcacg ctcgtgagcc acaactcgaa
     6001 gtcgttccag gcgaccgagc gcttcaagaa gaccttcggc gacgacgccg tgatcgtgct
     6061 cgtccagggc aacctgcaga agacggttct cacctcggac ctcgggcggc tcctgcggct
     6121 cgagggctgc ctgtcgggca atgttccgaa ggcgggtctg aaggacctgc cggccgagtg
     6181 cacgtggttc gcgcagcaca agcccgccaa ggtggtctac ggccccggca cgttcgtgaa
     6241 caccgcggcc gcgcggctgt cggagggctt cactcagcgc cagcagcagg agcagcaggc
     6301 ggcggacgcc gcggcgaaga tcgcgaggca gaaggccgcc aagcaggggc tctccgccgc
     6361 ggggcagaag aaggcggctg acgacgcgcg cgcggcggtg gagcagcagt tcaagctctc
     6421 gatcctcaag ctcgtgttgc gctacgggat cacgagcgtc ccggcgctcg acaacccgga
     6481 gttcgtctcg cagctcgtgt tcgacccgtc gcgcggggtc ggccagccga aggcgcgctt
     6541 cgcctacctg ttccccagct cgaactcggc cgtcgtgcag gtgcgcttca agcccgggct
     6601 gagcgactcg gagcggagcg ccgcgatcgc gaaggtgcgc caggcggcgc gcgcgtcgca
     6661 gttcaagctc cagaacggcg gccgctacgt cgtcacgggt gtgccggtcg tggtggacgg
     6721 cctcgccact gcggtgaagc actcgatctt catcctgctc gcggccgcca tcgtggtgat
     6781 ggcgggcacg ctcgcgctcg tcttccgctc gcggctgcgg ctgctgccgc tggcggtcgc
     6841 gcttgtggct gccgcgctca cgttcggcgc tgtgtcgctg gccggcggct cgctcacgat
     6901 ggcgtcgatc gcggtgctgc ccgtgctcat cggcctgtcg gtcgactatg cgatccagtt
     6961 ccaggcgcgc tacgacgagg cgcggctcga gggccggccg cccgaggaag ccgccccggc
     7021 ggctgcggcg gcgggcgggc ccacgatcgc gacggccggg ctcgcgaccg cggtcggctt
     7081 cctcgtgctg ctgctctcgc ccgtcccgat ggtgcgcggc ttcggcgcga tgctcgtgct
     7141 cggcatcgtg atcgccttcg cctgcgcgct caccgcgggc tttgccgcgc tcgtccgctt
     7201 ccaggacggc tcgccgggcg cgctgccggg actccgcgac cgcgccagcg cggctgggga
     7261 gcggatcgcg gacacgcgcg cgtggtcttg gctcgcgcac cacggacacc gcgggcgcga
     7321 gctcgcgctc gaccgcggca tccgcatcgt ggccacctcg atccggcggc cgcgcgcggt
     7381 gctcggcgca gcgctcgcaa tcgcggtgct cggctgggcg gctgacacgc agacgcacgt
     7441 gatctccgac gtgcgcgaac tggtgccgca gaacctccag gcgctgaagg acgtgaacac
     7501 gctgcagaag gagacgggcg tgtccggcga gatcgacgtg gtgctgaccg gcgacaagct
     7561 cgcgacgccg caggtgatcg attggatgac gcgcttccag tcgggcgtgc tgcaggcgca
     7621 cggctggacg accggcgcca cctgcgcaca gcggaagaac ccgccggaga tctgcccggc
     7681 cctgtcactg cccgacctct tccgctccgg cggggtcacc tcgcaggccc agatcgatgg
     7741 gctgctgcag gccgtgccac gctacttctc gcaagcggtg atctcaccgg atcagaagac
     7801 tgcgacgctc gcgttcggga tccgcctgat gccgctcgac cgccagaagc aggtgatcga
     7861 cgacatcgag cggcggctcg atccgcccgc cggcgttcag gcgtcggtcg ccggcctccc
     7921 tgtgctcgcg gccgaggcaa actcggcgct gtcctccacc gccaggcgcg cgctcacgct
     7981 gctcgccggg ctcgtcgccg tgttcctggt gctgctgatc gtgaggcggc gcctgcggct
     8041 cgcgacgatc ccgctgatcc cgatcgccct cgccaccggc tggtcggcgc tcgtgctctt
     8101 cctactcaga atcccgctca acccgatgtc ggcgacgatg ggtgcgctcg tgatcgcgat
     8161 ctccaccgag ttcagcgtgc tgctgtcctc gcgctacgag gaggagaggc gtgcgggcgc
     8221 cgccccggcg cgggcgctcg agctcacgta cgcctccacg ggcgcggcgg tgctggcgtc
     8281 gggcgtgacc gcgatcgcgg gcttcgccgc gctgatcgcg tccgacatcc gaatgctgcg
     8341 cgacttcggc atcgtcacgg tcgtggacct cacgctggcg ctgctaggcg tgctcgtggt
     8401 cctgcccgcg gcgctcgtgt gggcggagca gcacggcaag ttcaccttga gcgacatcga
     8461 tccgcgccgc ggcgtgcgag cgttgcgcga cgcggtccgc cggcgccggg cccggggcgg
     8521 caccgcgctg ccggcgcggc gggcgtgagc gaggaccgct tcggagacct cggtccctcg
     8581 ggccacgacg aggggcgcag cgccgcggag cggctcgccg acatggacga gcgccagctg
     8641 cgcgaggagc gcgagcggcg tccgcccgag gaggaccggc gcccgcgcgg gcgctactcg
     8701 tgggtgatcg ggatcgcgtt cgtgatcgtg atcgtggtcg cgggcctcaa ctcgattccg
     8761 cacagcggcg agggcgtgaa ggggccgaag cccggcgagc cactgccgaa gttcgcggcg
     8821 ccgctcgcga ccggcacgag caacgccgac gccaacgtga agcagaaggc gtcggattcc
     8881 gaccagctcg ggaagaagcc tgcgtgcgac gttcgagggc cgggcatctt caacctctgc
     8941 gacgccgaga acaagccgct ggtgctcacc ttcatcgtca cgcgcggggc gaactgcaag
     9001 ccgcagctcg atcgcgtgga gcaggtgcgg cggcaattcc cgggcgtcgg cttcgtgggc
     9061 gtggtgagcg gcgacgaccg cacgaaggtg gcgaagatcg tgcgcgagcg cggcctgcgc
     9121 taccccgtcg cggtcgatca cgacggcgag ctcgtgaacg tctacgggat cggcgtctgc
     9181 ccggcgacgg tgttcgcgaa gccggggggc aaggtgctgg tgacgaagct cggcaggttg
     9241 acggatcagc agctgagggc gcaggtgcag gcactcctga aggcgtcatg agcgagctgg
     9301 cgatcgagcg cggctgggtc gcagcgcccc tgcgagacga gttcccgttc ctaggactca
     9361 tgcacgt
//
LOCUS       DASVXS010000214         6083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_201999, whole genome shotgun sequence.
ACCESSION   DASVXS010000214 DASVXS010000000
VERSION     DASVXS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201999"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VF752_11975"
     CDS             complement(<1..871)
                     /locus_tag="VF752_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX8856305.1"
                     /translation="MAKAPTASAQTTGAQQAEDTAVWLERYGRMFTIRLFEGEMHRLF
                     LKGEVHGTTHLSSGQEAVPVGVCSALESSDYVTGTYRGHGHALAKGTDPEPLAAEMLG
                     RATGVCGGRAGSMNVIDLEHGLVGCFGIVGGSIGAATGAALAAKRNGGVAAAFFGDGA
                     ANQAYFHECMNFAAVHELPMLFVCENNFYGEFTPMQKVTAGAEIAARARSYAVPGQVV
                     DGNDVFAVHAAALEAAQRARSGGGPTLLECQTYRHYGHSKSDPAKYRPQEEIERWLAR
                     DPLTIAKTKLQELG"
     gene            complement(1038..2012)
                     /locus_tag="VF752_11980"
     CDS             complement(1038..2012)
                     /locus_tag="VF752_11980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8856306.1"
                     /translation="MDDGNVKRHRLGWAGTGRMGYALATRLLERGCDLAVYNRTRSKA
                     EPLAELGATIVDTPAELSDREIVFTMVAGPDDFRELVLGATGLLSRDGGPRIIVDSTT
                     VSPDTSAEVREKAKARGTDLLAAPVSGNPRVVESGKLTFVVSGPEEAYREVSPYLDVL
                     GAGATYVGDGELARLAKICHNLLLGVVSQSLAEIAVLAEKGGMSRAAFFEFLNKSVMG
                     SMFTRYKTPAYVNLDFHATFTPLLLRKDFDLGFAAAREMDVPMPVAAATQQVIQSLIG
                     SGYREQDFATLLELQARHSDLELVSEDAPVSDGLGAEADTQLSAQPAG"
     gene            complement(2164..3969)
                     /locus_tag="VF752_11985"
     CDS             complement(2164..3969)
                     /locus_tag="VF752_11985"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX8856307.1"
                     /translation="MLEAATRTNNQRLLDWVDEVAELAQPEAIHWCDGSAAEYEHLCQ
                     EMIEAGTLERLSEEQRPGSYLARSDPDDVARVEDRTFICSQTAEAAGPTNNWRDPDEM
                     RRTLTDLFRGSMRGRTMYVVPFSMGPIGAPIAHIGVQLTDSPYVAASMRLMTRMGRAA
                     LEALGSNGSFVPCLHSVGHPLADGEHDVPWPCNADNKYIVHFPETREIWSFGSGYGGN
                     ALLGKKCLALRIASVMARDEGWLAEHMLILKLTSPEGEVKYVAGAFPSACGKTNLAMM
                     IPTLEGWKVETIGDDIAWMRFGEDGRLYAVNPEAGFFGVAPGTGADTNPNAMKMLERN
                     TIFTNCARTDDGDVWWEGMTGEPPAHAVDWRGAEWTPESDSTAAHPNARFTVAATQCP
                     SLAPEWDDPAGVPIDAIIFGGRRSTVVPLVTEARDWDHGVFLAATMSSETTAAAAGQV
                     GKLRFDPFAMLPFCGYNMAEYFAYWLSLKEREGARLPRLFYVNWFRKDEKGNFLWPGF
                     GDNSRVLAWIFRRCDGKAEAQDSPLGMVPAEGEIDVTGLDVSAEDMRALLTVDGGELK
                     AQLPQMHEHFATFDSRLPQVLRDELDALEQRVSAG"
     gene            4104..5477
                     /locus_tag="VF752_11990"
     CDS             4104..5477
                     /locus_tag="VF752_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX8856308.1"
                     /translation="MGLHTYGTMGVDWEERVRFDRLREERLARVSSLLERSEMGALLC
                     FDMTNIRYITATHIGTWAQDKLNRFCLLTRGDQPIMWDFGSAARHHQLYNPWLGDGRS
                     RAGISTLRGAMSPESGRAENVARKIRVELEERGLLGEPVGVDAVEPAVLFALQAEGIE
                     VVDGQQVMQEARMIKTRDEVTLLNQACAMVDAAYEELYRAMKPGMRENECVGLVNKVL
                     YDMGSEFVEGVNAISGERCNPHPHVYTDRALRPGDPAYFDILHSFMGYRTCYYRTFAV
                     SSASRALVDAYKRCRYYLDAAIELVRPGVTTAEVVSVWPRAEEFGFADEEAAFALQFG
                     HGVGLAIWEKPIFSRLVSLDHPEEIQEGMVFALETFWPASDGWSAARIEEQLVVTADG
                     CEVITRFPAEDLVIAGKPYYTVGGTLPLERETQSNLNTTLDRETSTAAPGNGRMPAAD
                     SGAPALR"
     gene            5474..>6083
                     /locus_tag="VF752_11995"
     CDS             5474..>6083
                     /locus_tag="VF752_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856309.1"
                     /translation="MSAELVAQPYPRFSDDEMARRAGVLDAMMAERGVDHAVIYGANR
                     FGSAVGWLTRWPVTREAVVVHTAGQRDALLVNFYNHVPNAQRIATEADVRWAGERSAT
                     TAVEELERRGARGRRIGVIGPLAYRAHATLEALAAEVVDMNGDYTRLRLMKSAEEIEW
                     VRVAAGMTDDAVRSVHDNAVPGIDERELGDIVERAYVTRGGTT"
BASE COUNT          935 a         2067 c         2122 g          959 t
ORIGIN      
        1 cgcccagctc ctgcagcttc gtcttcgcga tcgtgagcgg atcgcgcgcg agccagcgct
       61 cgatctcctc ctgcgggcgg tacttggccg ggtcggactt cgagtgcccg tagtgccggt
      121 acgtctggca ctcgagcagc gtcggcccgc cgccggagcg cgcccgctgc gccgcctcga
      181 gagctgcagc gtggaccgcg aagacgtcgt tgccgtcgac cacctggccg ggcacggcgt
      241 aggacctcgc gcgggcggcg atttcagcgc cggcggtgac cttctgcatc ggcgtgaact
      301 cgccgtagaa gttgttctcg cacacgaaca gcatcgggag ctcgtggacc gccgcgaagt
      361 tcatgcactc gtggaagtac gcctggttgg cggcgccgtc gccgaagaat gcggcggcca
      421 cgccaccgtt gcgcttcgca gcgagcgccg cgccggttgc cgcgccgatg ctgcccccca
      481 cgattccgaa gcagcccacg agcccgtgct cgaggtcgat cacgttcatc gagcccgccc
      541 gcccgccgca cacgcccgtc gcccgtccga gcatctcggc cgcgagcggc tccgggtcgg
      601 tgcccttggc gagcgcgtga ccgtggccac gataggtgcc tgtcacgtag tcgctcgact
      661 cgagagcgga gcagaccccg acgggcacgg cctcctgccc cgacgacaga tgagtggtcc
      721 cgtgcacctc tcccttgagg aagagccggt gcatctcgcc ctcgaagagc cggattgtga
      781 acatgcgtcc gtagcgctcg agccaaactg cggtgtcctc agcctgctgc gcgcccgtgg
      841 tctgggcgct ggcggtcggg gccttcgcca cccgtgcctc ctcctcgacg acgttgccgg
      901 cggtaggtta gcgcaaacgt ttgcagtgag ccaaggcgtg cgaccaccgc atcctctacg
      961 gcgccgacgg cgcacagtac ctcgcgcggg tgcggcagct gctcgagcag cccctagctc
     1021 tgactctctg agccggctca gcccgcgggc tgcgcggaca gctgcgtatc ggcctcggcg
     1081 ccgagtccgt cgctgaccgg agcgtcctcc gaaaccagct cgagatccga gtgccgcgcc
     1141 tgcagctcga ggagcgtggc gaagtcctgc tcgcggtagc cggagccgat cagcgactgg
     1201 atcacctgct gggtggcggc cgcgaccggc atcggcacgt ccatctcacg cgccgcggcg
     1261 aagccgagat cgaagtcctt ccggagcagc agcggggtga acgtagcgtg gaagtcgagg
     1321 ttcacgtacg ccggcgtctt gtagcgcgtg aacatcgagc ccatgacgct tttgttgagg
     1381 aactcgaaaa acgcggcgcg ggacatgccg cccttctcgg ccagcaccgc gatctccgcg
     1441 agcgactgcg acaccacccc gagcagcagg ttgtggcaga tcttcgccag gcgcgcgagc
     1501 tcgccatcgc cgacgtaggt agcgcccgcg ccgaggacgt cgaggtacgg cgacacttcg
     1561 cgataggcct cctcggggcc ggagacgacg aacgtcagct tcccgctctc gaccacgcgc
     1621 gggttgccgc tcacgggggc ggccaacaga tccgtgccac gtgcctttgc cttctcacgc
     1681 acttccgcgg acgtgtcggg cgagacggtg gtggagtcga cgatgatgcg cgggccgccg
     1741 tcgcgcgaca gcagtcccgt ggctccgagc acgagctccc tgaagtcgtc gggtccggcc
     1801 accatcgtga agacgatctc gcgatcggac agctcggcgg gcgtgtcgac gatcgtggcg
     1861 ccgagctcgg cgagcggctc ggccttcgat cgcgtgcggt tgtacacggc gagatcgcag
     1921 ccccgctcga gcaggcgggt ggccagcgca taccccatcc gaccggttcc agcccatccg
     1981 agccggtgcc gcttcacgtt gccgtcatcc atctactttc ccctcaatcg gcgctccggc
     2041 tcagctcctg cctcgcctgg ccggtcgtct gttgcccgtg tgatgcgtcg ctgcaatcgt
     2101 ttgcaggcta ccgaacagga atcacgccgt caacaggtga gatccgagcc cgggctcggc
     2161 gcgctacccg gcggacacgc gctgctcgag cgcgtcgagc tcgtcacgga ggacctgcgg
     2221 caggcggctg tcgaaggtcg cgaagtgctc gtgcatctgc ggcagctgcg ccttcagctc
     2281 acccccgtcg accgtgagca gggcgcgcat gtcttccgcc gacacgtcga gcccggtgac
     2341 gtcgatctcg ccctccgccg gcaccatgcc gagcggcgag tcctgcgcct ccgccttgcc
     2401 gtcgcagcgg cggaagatcc acgcgagcac tcggctgttg tcgccgaagc cgggccatag
     2461 gaagttgccc ttctcgtcct tgcggaacca gttcacgtag aacaggcgcg gcaggcgcgc
     2521 gccctcgcgc tccttgaggc tgagccagta cgcgaagtac tccgccatgt tgtagccgca
     2581 gaacggcagc atcgcgaacg ggtcgaagcg cagcttcccg acctggccgg cggccgccgc
     2641 ggtggtctcc gaggacatcg tggcggcgag gaacacgccg tgatcccagt cgcgtgcctc
     2701 ggtcacgagc ggcaccaccg tggaacggcg accgccgaag atgatggcgt cgatcggcac
     2761 tccggccggg tcgtcccact ccggggcaag cgacggacac tgcgtggcgg ccacggtgaa
     2821 gcgcgcgttc gggtgcgcgg ccgtgctgtc ggactcgggg gtccattcgg ccccacgcca
     2881 atcgacggcg tgcgccggcg gctcgccggt catgccctcc caccagacgt cgccgtcatc
     2941 ggtgcgcgcg caattggtga agatcgtgtt ccgctcgagc atcttcatcg cgtttgggtt
     3001 ggtgtcggcg ccggtgccgg gggccacgcc gaagaagccc gcctcgggat tcaccgcgta
     3061 gagccgcccg tcctcgccga agcgcatcca ggcgatgtcg tcgccgatcg tctcgacctt
     3121 ccaaccctcg agggtcggga tcatcatcgc gaggttggtc ttcccgcacg cagacgggaa
     3181 ggcgcccgcg acgtacttca cctcgccctc gggagaggtg agcttgagga tcagcatgtg
     3241 ctcggcaagc cagccctcgt cgcgggccat gaccgaagcg atccgcagcg caaggcattt
     3301 cttgccgagt agcgcgttgc cgccgtagcc cgagccgaac gaccagatct cacgggtctc
     3361 ggggaagtgg acgatgtact tgttgtcggc gttgcagggc cacggcacgt cgtgctcgcc
     3421 atccgcgagg ggatgcccca ccgagtgcag gcacggcacg aaggagccgt tggagccgag
     3481 cgcctcgagc gcggcgcggc ccattcgcgt catcaggcgc atgctcgcgg ccacgtaggg
     3541 cgagtccgtg agctggacgc cgatgtgcgc tatcggcgcg ccgatcggac ccatcgagaa
     3601 cggcaccacg tacatggtgc ggccgcgcat cgaaccgcgg aacaggtcgg tgagcgtgcg
     3661 gcgcatctcg tccgggtcgc gccagttgtt ggtcggcccc gctgcctccg cagtctgcga
     3721 gcagatgaac gtgcggtcct ccacgcgcgc gacgtcgtcg gggtcggagc gcgcgagata
     3781 agacccgggg cgctgctcct ccgacaggcg ctcgagcgtg cccgcctcga tcatctcctg
     3841 gcagagatgc tcgtactctg ccgcggaccc gtcacaccag tggatcgcct cgggctgggc
     3901 gagttcggct acttcgtcga cccagtcgag gaggcgttgg ttgttggtgc gagtggctgc
     3961 ttcgagcacg gttgggcgaa ctccttcgtc gatcacttga ctcgtcagtc aatcatttcc
     4021 ggtggcatga agggcgcgct agtatgctca cgttagctgc aatcgattgc aagcatttcg
     4081 gagaatttgg cgcgaggtag gcgatgggac tgcacacgta cggaaccatg ggggtcgact
     4141 gggaggagcg tgtgcgcttc gaccggttgc gcgaggagcg gctcgcgcgc gtgtcctcgc
     4201 tgctcgagcg gtcggagatg ggggcgctgc tgtgcttcga catgacgaac atccgctaca
     4261 tcaccgcgac ccatatcggc acctgggcgc aggacaagct gaaccgcttc tgtctactga
     4321 cccgcggtga ccagccgatc atgtgggact tcgggtctgc ggcgcgccac caccagctct
     4381 acaacccctg gctcggggac ggccgctcgc gtgccggcat ctccacgctg cgcggcgcga
     4441 tgtcgccgga gtcgggccgc gcggagaacg tggcccgaaa gatccgcgtg gagctcgagg
     4501 aacgaggtct gctcggagag cctgtcggcg tcgacgccgt cgaacccgcg gtgctgttcg
     4561 cgttgcaggc cgagggcatc gaggtggtgg acgggcagca ggtgatgcag gaggcgcgca
     4621 tgatcaagac gcgggacgag gtcacgctcc tgaaccaggc ctgcgcaatg gtcgacgccg
     4681 cctacgagga gctctaccgg gccatgaagc cggggatgcg cgagaacgag tgcgtggggc
     4741 tcgtgaacaa ggtgctctac gacatgggct cggagttcgt ggagggcgtg aatgcgatct
     4801 cgggcgagcg ctgcaacccc cacccgcacg tctacaccga cagggcgctg cgtcccgggg
     4861 acccggccta cttcgacatc ctccacagct tcatgggcta ccgcacgtgc tactaccgca
     4921 cgttcgcggt ctcgagcgcc tcgcgcgcgc tcgtcgacgc ctacaagcgc tgccgctact
     4981 acctcgacgc cgccatcgag ctcgtccggc ccggcgtgac gaccgccgag gtcgtgtcgg
     5041 tctggccacg tgccgaggag ttcggcttcg cggacgagga ggccgccttc gcgctccagt
     5101 tcggccacgg cgtgggcctc gccatctggg agaagccgat cttcagccga ctggtgtcgc
     5161 tcgaccaccc ggaggagatc caggagggca tggtgttcgc cctcgagacg ttctggccgg
     5221 cgagcgacgg ctggtccgca gcccgcatcg aggagcaact cgttgtgacc gccgacggct
     5281 gcgaggtgat cacgcgcttc ccagcggagg acctcgtgat cgcgggcaag ccctattaca
     5341 cggtgggagg cacgctgccg ctcgagcgcg agacgcagtc gaacctcaac accacgctcg
     5401 atcgggagac gagcacggcg gcgccgggga acggccgcat gcccgccgcc gattccgggg
     5461 cgcccgcgct gcggtgagcg cagagctggt cgcgcagccg tacccgcgct tcagcgacga
     5521 cgagatggcc cggcgcgcgg gcgtgctcga cgccatgatg gccgagcggg gcgtggacca
     5581 cgcggtgatc tacggcgcca accgcttcgg ctcggccgtg ggctggctca cgcgctggcc
     5641 ggtcactcgc gaggcggtcg tggtgcacac cgcggggcag cgtgacgcgc tgctcgtgaa
     5701 cttctacaac cacgtgccga acgcgcagcg catcgcaacc gaggccgacg tgcgctgggc
     5761 cggggagcgg tcggccacca ccgccgtgga ggagctcgag cggcgagggg cacgggggcg
     5821 gcggatcggc gtgataggcc cgctcgcgta tcgggcgcac gccacgctcg aggcgctagc
     5881 cgccgaggtc gtggacatga acggcgacta cacgcgattg cgcctgatga agtcagcgga
     5941 agagatcgag tgggtgcggg tagcggcggg catgaccgac gacgcggtgc gctcggtgca
     6001 cgacaacgcc gtgccaggga tcgacgagcg cgagctgggc gacatcgtgg agcgcgccta
     6061 tgtgacccgt ggtggcacga cgc
//
LOCUS       DASVXS010000215         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_736722, whole genome shotgun sequence.
ACCESSION   DASVXS010000215 DASVXS010000000
VERSION     DASVXS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736722"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..303
                     /locus_tag="VF752_12000"
     CDS             121..303
                     /locus_tag="VF752_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856310.1"
                     /translation="MADSPARVTPASVMPFAHIAGVPAEELLPAAYGAAGVLVALRVL
                     ARHRLLRRGRRRPQGR"
     gene            366..659
                     /locus_tag="VF752_12005"
     CDS             366..659
                     /locus_tag="VF752_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856311.1"
                     /translation="MAIDSDGAAVEFANLHAEHADLALVDALARLQLAARRRGLTLCV
                     RDPCQELTGLLELVGLSGVIAAAPELLLDDCRKPEGGEQVGEEEVVDGGDPPV"
     gene            complement(573..1541)
                     /locus_tag="VF752_12010"
     CDS             complement(573..1541)
                     /locus_tag="VF752_12010"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8856312.1"
                     /translation="MATTLSPAVEQELESHRKELTGYCYRMLGSGFEAEDAVQETMVR
                     AWRGIDRLEERAALRSWLYRIATNVCLDMLQSSQRRARPMDLASPSTADTALGPEVPE
                     NNWVLPVSDGRVIPSDGDPAELASARETIRLAFVAALQHLPPKQRAVLILREVLRWQA
                     SEVAELLDTSVASVNSALQRARATIDTVAPDASGPVAVDDEQKELLARYVDAFERYDV
                     GELVTLLHEDATWEMPPFDLWLQGPEEVSRWMLGPGAGCANARVLTTMANGCPALAIY
                     KTAEPGVWRPFNLMVLEMSDGRVAAVHNFLFTDLFPAFGLPTVIEE"
     gene            1634..>3031
                     /locus_tag="VF752_12015"
     CDS             1634..>3031
                     /locus_tag="VF752_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8856313.1"
                     /translation="MTTEATPIQSTREDSRARWIALYVLCAGVLMIVLDVTVVNVALP
                     SIQSDLDFSQSSLAWVVNAYLIAFGGLLLLAGRLGDLISRRDVFLVGLAIFTVASFAC
                     GVSQSQEMLVAARFVQGIGGALASAVVLGMIVTMFPEPAEQAKAIGVYGFVASGGGTV
                     GLLAGGAVTQAISWHWIFFINIPIGIFTAVMAVRLLERDRGIGVGRGADILGAVLITG
                     ALMLLVYTIVKPAAEDGWGAGRTLALGAASLALIAAFVVREATARNPLIPLRIFKSRN
                     VTGANVIQAVSVAGMFAMFFMGALYFQRVLGYDALETGLAFLPATLVMGTLSVRYSER
                     FVMRFGARATLIPGLGLILAGLLLFTLSPVDGSYFVHGLPVVVLIGAGAGVAFPALMT
                     LAMSGATPSDAGLASGLINTTAQVGGALGLAVLATIATTKTHSLRDAGDSVASALNGG
                     YHVAYLIGALLVGVAI"
BASE COUNT          424 a         1037 c         1071 g          499 t
ORIGIN      
        1 gccgctgagc ggaccgatta gttccggcga gccggggcgt cctagtggca atccctgcat
       61 gaatcgtctg aaatggaggc gagggatcgt gactgcccac accgttggca cgcgcgagga
      121 atggctgaca gcccggcgcg agttactccg gcaagcgtga tgcccttcgc tcacatcgcc
      181 ggtgtgcccg ctgaggagtt gctgcccgcc gcgtacggag cggcgggcgt gctcgtggcg
      241 ctgcgggtcc tcgcccgcca ccgcctgctc cgccgcgggc gtcggcgccc gcaagggagg
      301 tgagccggca gcgcggcccg gcggacacca tggggcgaga gggagaaggg ggaacgatcc
      361 agctggtggc catcgacagc gacggcgccg ccgtcgagtt cgcgaacctg catgccgaac
      421 acgcggacct cgcgctcgtg gacgcgctgg cgcggctcca gctcgctgcc cggcgacgcg
      481 gcctgacgct gtgcgtccga gacccgtgcc aggagctcac gggcctgctc gaactggtcg
      541 ggctcagcgg cgtgatcgcc gccgcacccg agctactcct cgatgactgt cggaagcccg
      601 aaggcgggga acaggtcggt gaagaggaag ttgtggacgg cggcgacccg cccgtctgac
      661 atctcgagca ccatcaggtt gaacggcctc cagaccccgg gctcggccgt cttgtagatg
      721 gcgagcgcgg ggcatccgtt ggccatggtg gtgagcaccc gcgcgtttgc gcaaccggcg
      781 cccggtccga gcatccagcg gctgacctcc tcggggccct gcagccagag gtcgaagggt
      841 ggcatctccc acgtggcgtc ctcgtggaga agcgtgacga gttcaccgac gtcgtagcgc
      901 tcgaaggcgt cgacgtagcg cgccagcagc tccttctgct cgtcgtcgac cgcgaccgga
      961 ccggacgcgt caggggccac ggtgtcgatc gtcgcacggg cgcgctggag cgcgctgttc
     1021 accgacgcga cgctcgtgtc gagcagctcg gccacctcgc tggcctgcca gcgcagcacc
     1081 tcgcgaagga tcagcaccgc gcgctgcttc ggcggcaggt gctgcagcgc ggcgacgaac
     1141 gccagccgga tcgtctcgcg agccgacgcg agctccgccg gatcgccgtc ggagggaatc
     1201 acccgcccat ctgacacggg cagcacccag ttgttttcgg ggacctcggg gccgagcgca
     1261 gtgtcggcgg tcgagggcga cgccagatcc atcggacgcg cccgccgctg cgacgactgc
     1321 agcatgtcga ggcacacgtt cgtggcgatc cggtagagcc acgagcgcaa ggcggcgcgc
     1381 tcctcgaggc gatcgatgcc gcgccaggcg cgcaccatcg tctcctggac ggcgtcctct
     1441 gcctcgaaac cggagccgag catccggtag cagtaaccgg tcagctcctt gcggtgcgac
     1501 tcgagctctt gctcgactgc aggggagagc gtggtggcca tgagcgacat cctaggcgct
     1561 ccgccgccgg accgattagt ttgccccgcc cccctcgtct gagggttgga ccccaaggac
     1621 caggagaccg catatgacca ccgaagccac gccgatccag agcactcgag aggacagccg
     1681 cgctcgctgg atcgccctct acgtgctctg cgccggtgtg ctgatgatcg tgctcgacgt
     1741 gaccgtcgtg aacgtggcgc tgccctcgat ccagagcgat ctcgacttct cccagtcgag
     1801 cctcgcctgg gtggtgaacg cgtacctgat cgccttcggc ggcctgctgc tgctcgccgg
     1861 ccggctcggc gacctgatat cgcggcgcga cgtgttcctc gtcggcctcg ccattttcac
     1921 cgtcgcgtcg ttcgcctgcg gcgtctcgca gagccaagag atgctcgttg cggcgcgttt
     1981 cgtgcagggc atcggcggcg cgctggcgtc tgcggtcgtc ctcggcatga tcgtgacgat
     2041 gttccccgag cccgccgagc aggcgaaggc gatcggggtc tacggcttcg tcgcgtcggg
     2101 cggcggcacg gtcgggctcc tcgccggcgg cgcagtcacg caggcgatca gctggcactg
     2161 gatcttcttc atcaacatcc cgatcgggat cttcacggcg gtcatggcag tgcgcctgct
     2221 cgagcgcgac cgcggcatcg gcgtcgggcg cggcgccgac atcctgggag ccgtgctgat
     2281 caccggcgcg ctgatgctgc tcgtctacac gatcgtgaag ccggcggccg aggacggctg
     2341 gggcgcgggc cggacgctcg cgctcggcgc cgcgtcgctc gcgctgatcg cggcgttcgt
     2401 cgtacgcgag gcgaccgccc gcaatccgct gatcccgttg cggatcttca agtctcggaa
     2461 cgtgaccggc gcgaacgtga tccaagcggt ctcggtcgcc gggatgttcg cgatgttctt
     2521 catgggcgcg ctctacttcc agcgcgtgct tggctacgac gcgctcgaga ccggtctggc
     2581 attcctgccc gccacgttgg tgatgggcac gctctcggtg cgctactcgg agcgcttcgt
     2641 gatgcgcttc ggcgctcgag caacgctgat ccccggcctc ggtctgatcc tggcgggcct
     2701 gctgctgttc acgctctctc cggtggacgg cagctacttc gtccacggcc tgcccgtggt
     2761 ggtcctgatc ggcgcgggcg caggcgtcgc gttcccggcg ctgatgacgc tcgcgatgtc
     2821 gggcgcgacg ccgagcgatg ccgggctggc gtccggcttg atcaacacga cggcgcaggt
     2881 cggcggcgca ctggggctcg cggtgctcgc cacgatcgcg acaacgaaga cgcattcgct
     2941 gcgcgacgcg ggcgactcgg ttgcatcagc gctgaacggt ggctaccacg tggcctacct
     3001 gatcggggcg ctgctggtcg gggtcgcaat a
//
LOCUS       DASVXS010000216         7421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1333408, whole genome shotgun sequence.
ACCESSION   DASVXS010000216 DASVXS010000000
VERSION     DASVXS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333408"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF752_12020"
     CDS             complement(<1..435)
                     /locus_tag="VF752_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856314.1"
                     /translation="MTDPPEITAAELEQLGLYDPHDDHAAERRQLLQDLASLGAAPED
                     LVAYREELPGLASVLAIRGGAALTLEEAAERSDLPAEKLLQMNRGAGFPDPGTGDRVF
                     GERFVELAGGMAAAETLFGEEAVMQLLRVMGSSMARVADALVS"
     gene            complement(454..903)
                     /locus_tag="VF752_12025"
     CDS             complement(454..903)
                     /locus_tag="VF752_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8856315.1"
                     /translation="MTTLEQDHKVIARRALEEVCARGDFAKAEQFYSPSFRDHVNELE
                     FHGLEGVRKSVGLYRSVFPDLEIEVVDQVIDGDEVASRWVARGSNRGRRATLEGITIS
                     RIEDGKIVEDWTVSDNVGMLRRLGLWRTLRLLAAQARDRLSSGSREA"
     gene            complement(920..1288)
                     /locus_tag="VF752_12030"
     CDS             complement(920..1288)
                     /locus_tag="VF752_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX8856316.1"
                     /translation="MAGNGRVVVGCTHGEDDPDNVAVAYLTAGAALDQGKEVVMWLTS
                     DGVRLGLQGYADRIREDKDPPAARLHAQFIEKGGRFYVCPICFNERDLDADDLVENAE
                     LKGAAALMEFAGNGAVTFTY"
     gene            complement(1337..2002)
                     /locus_tag="VF752_12035"
     CDS             complement(1337..2002)
                     /locus_tag="VF752_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8856317.1"
                     /translation="MPSEGNKNALFEAIALMGKGLASPRRLELLDLLAQAPRRVDDLA
                     RASGQSTANTSQHLQAMHAAGLVTRTREGTSVRYSLAGDEVLSLWLALRDVSVARLAE
                     VECAARDYLGDEVESIGREELIARLRTGDIVLVDVRPAEEFQAGHINGARSIPLDELE
                     DRLADLPADREVVAYCRGPFCAYAHEAVRRLQAAGRPARRLAEGWPEWRLAAEPTKRT
                     RAA"
     gene            2073..2837
                     /locus_tag="VF752_12040"
     CDS             2073..2837
                     /locus_tag="VF752_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8856318.1"
                     /translation="MYFRQLLNDETACASYLLGCKTRATFAVFEPHVDLVDEYIALAE
                     AEGAKIVAVFETHVQADHVSGLPELVARTAATAYVPGGSGVEFEHRALRDREKVTLGN
                     TEVEAIATPGHALAHHSYLVTDHRRGDEPWFVLTGDALLVGDAGRPDLHAHGEHTAEQ
                     MARSLYRSLTERLLALPDDLVLYPSHYSGSVCGRGLSSNPISTIGFERRHNPALQLGS
                     EDAFVEALLRDVPPAPQQQAAIVAANRSGRPLAEVG"
     gene            2834..4111
                     /locus_tag="VF752_12045"
     CDS             2834..4111
                     /locus_tag="VF752_12045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8856319.1"
                     /translation="MTASPVRLGLRENAAQFSLLVAINVFVGAMVGLERSTLPLVGRD
                     DFGLRSSAAVLSFIVAFGVAKAFTNLAAGALAERAGRRRLLVAGWAVALPVPLLIAVA
                     PSWGWIVAANALLGINQGLAWSMTVVMKIDLVGPRRRGLALGLNESAGYGGVALAAGL
                     GGWLAGSFAARDVLVVAGAAIAIIAFLLSLLFVKDTGAHVELEQSRRTGDRDVAAPPL
                     RSAFAAATYGDPTLRSCSQAGLINNLNDGLAWGLVPLYLAAHGRSIGEIGLVAALYPG
                     VWSVAQIPAGHWSDVIGRKPLIVGGMLVQAVALALLALSSGAVPAAAVAAVLLGAGTA
                     LVYPTLIAAISDAVSPVARASVVGVYRFWRDSGYVFGGLIAGLVADALGYGGAISLVA
                     ALTAASGLWVLRDMRPSGTSARLLSRGPTGSRT"
     gene            complement(4065..6923)
                     /locus_tag="VF752_12050"
     CDS             complement(4065..6923)
                     /locus_tag="VF752_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856320.1"
                     /translation="MLPQIGRHAAADQRADAAKSRTLAPPRPLAPGDGAQGPAMPAFS
                     WGAVRSAAKYEFQLSADRTFNSAIKATQTLNTFATIDKSLTDGTYYWRVRSISSKDVA
                     GEWSRSRAYTKSWTDAPHLLSPADNATISYPDTPLVLHWSAVPRASKYLVYVATDPAL
                     GSLVVSRTGGKPVETSGTSFAVPASLAPGRYYWGITPEDTGGHDGEPSAVGSFDWSWG
                     TGTSTTVEDATGSHESDGAGRPKVLSPNLSWGVVPGAARYEVEVNTFSDFPPGSKVCC
                     NELTTGTSLSPTRFLANNVYHWRVRAIDLNGNAGGWNVGPDFRKAFDDVTPTIPNLTL
                     RDNTNTPLAWGASTAAPLLTWDPVPGASSYELDVAPWNSVISNCDFGSSATKTYITAT
                     TAWTPLADSSSTNPGPLSWPGVSTNSGLGSGDKCVRVMARTDKDLKNVDVVSGATQQG
                     GSLGPAFTYTPVTGSGPLGDAQESDYLSMTTPSSTTDMPVFRWKPIAGASSYYVVVSK
                     DSSFTNVVDVALTKAPAYAPRRGTLARTYSDENTDYYWQVLPAAGGSGTLCPCTWDTG
                     AEHPQRFQKSSIPPTPLSPTGGSGVGEQPTFRWSLANANFPSQPATQGAKTYRLQVSG
                     DPTFSDPIDDITTDSTSYTSTSTYPADTILYWRVRVNDENNVALNWSSIATFRRTLRT
                     PSLSADNPTVGPDVPVVRWDSVSGATSYTLHVEQGDGTAKSFDTRSTAFTPTGFFGVG
                     NVRWQVRANFPKASSGETAGPFSDLQTFTRQIAAPTGVRWTNTPRHVLLSWDPSTNIG
                     TRSYRAQISKTNSFSTLVDSHVTENTSYAPLLTQSGFKDGGTLYWRVASLDERGTLGA
                     WTVRTLTLPGRMNLKIEGSLRLRRSSVVRITALNVRGRPIRGARVSVSGIGVSKRAKR
                     TGKRGTVAFKLRASRKGSVVFTGSKSGYQSGRVTIVVR"
     gene            complement(7052..>7421)
                     /locus_tag="VF752_12055"
     CDS             complement(7052..>7421)
                     /locus_tag="VF752_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856321.1"
                     /translation="RGEHGDRRSFSAVVAAALVISPVVWLHYFALLLVPVALAAPRLS
                     RLWALPALFWIVIWLPGHAVFVASGDQAGAQVGIAPSLGRLLLAIALLAATFVVTAMP
                     HRVSRPLDLRPGWTRPSASG"
BASE COUNT         1082 a         2544 c         2550 g         1245 t
ORIGIN      
        1 ggacacgagc gcgtcggcga cgcgcgccat ggaggagccc atgacgcgca gaagctgcat
       61 taccgcttcc tcgccgaaca gcgtctcggc ggccgccatc ccgccagcca gctcgacgaa
      121 gcgctcgccg aacactcggt cgccggtacc cgggtcgggg aagccggcgc cgcggttcat
      181 ctgaagcagc ttctccgccg ggaggtcgga gcgctcggcc gcctcttcca gcgtcagcgc
      241 ggcgccgccg cggatagcca gcacggacgc gaggccgggc agctcctccc ggtacgcgac
      301 cagatcctcc ggcgcggcgc ccaggcttgc caggtcctgc agcagctggc ggcgctcggc
      361 ggcgtggtcg tcgtgagggt cgtaaagccc gagctgctcg agctccgcag cggtgatctc
      421 aggcgggtcc gtcaccgcag cacgcggcaa ccgtcacgcc tcgcgcgacc ccgaactcag
      481 gcggtcgcgc gcctgcgccg ccagcaaccg cagcgtccgc cacaggccca gccggcgcaa
      541 cattcccacg ttgtcggaga ccgtccagtc ctccacgatc ttgccgtcct cgatgcggct
      601 gatcgtgatc ccctcgagcg tcgcgcggcg accccggttg ctaccgcgcg cgacccaccg
      661 cgaggccacc tcgtcgccgt cgatcacctg gtcgacgacc tcgatctcga ggtccgggaa
      721 caccgaccgg tagaggccga cagacttcct aacgccctcg agcccgtgga actcgagctc
      781 gttcacgtgg tctcggaagc tcggactgta gaactgctcc gccttcgcga agtcgccgcg
      841 cgcgcacacc tcttccagcg cgcgccgcgc aatcaccttg tggtcctgct cgagcgtcgt
      901 catcgcgcgg agtatgccgt cagtaggtga aggtgacggc gccgttcccg gcgaactcca
      961 tcagggcagc ggcgcccttc agttcggcgt tctcgaccag gtcgtccgcg tcgaggtcgc
     1021 gctcgttgaa acagatcgga catacgtaga agcggccgcc cttctcgatg aactgcgcgt
     1081 gtagccgggc ggccggtgga tccttgtcct cgcggatccg atccgcgtac ccctggaggc
     1141 cgagccgcac gccgtctgag gtcagccaca tcacgacttc cttgccctgg tcaagcgcag
     1201 caccggcggt caagtaagcg accgcgacgt tgtctggatc gtcctcgccg tgcgtgcagc
     1261 cgaccaccac tcgtccgtta cctgccacta ctccccctct cgtttgacag cgcgaatcct
     1321 cgatcctctc agaacctcag gcggctcgtg ttcgcttggt gggctccgcc gcgagccgcc
     1381 attcgggcca gccctcggca aggcgccgtg cgggtcggcc cgctgcttgc agacggcgaa
     1441 ccgcctcgtg ggcgtaggcg cagaacgggc cgcggcagta ggcgacgacc tcccgatcgg
     1501 caggcaggtc ggccagccga tcctcgagct catcgagcgg gatcgatcgc gcgccattga
     1561 tgtggccggc ctggaactcc tcggcggggc ggacgtcgac gagcacgatg tctcccgttc
     1621 gcagccgggc gatcagctcc tcgcgcccga tgctctcgac ttcatcgccg agatagtcgc
     1681 gtgccgcgca ttccacctcg gcgagccggg ccaccgagac gtcgcgcagg gctagccaca
     1741 gtgacagcac ctcgtcgccg gcgagcgaat agcggacgct cgtgccctcg cgggtacgtg
     1801 tcacgaggcc cgcggcgtgc atggcctgca agtgctggga ggtgttcgcg gtcgattggc
     1861 cgctcgcccg cgccaggtcg tcgaccctgc gcggcgcctg cgcgagcagg tcgagcagct
     1921 cgagccgccg agggctggcg aggcccttgc ccatcagtgc gatcgcctcg aacagcgcgt
     1981 tcttgttgcc ctcgctaggc atccgttgct attcaataga tcacttgata agttgtcaag
     2041 ggcttggaat gactggactt gctggaggcc ccgtgtactt ccgacagctg ctcaacgacg
     2101 aaaccgcgtg cgcctcgtac ctgctcggct gcaagacccg cgcaaccttc gcggtgttcg
     2161 agccgcacgt cgacctcgtg gacgagtaca tcgcgctcgc cgaggcggag ggcgcgaaga
     2221 tcgtcgccgt ctttgagacg cacgtgcagg cggatcacgt ctcgggcctg cccgagctgg
     2281 tcgcgcgcac cgcggccacc gcatacgtgc cgggcggctc aggcgtggag ttcgaacacc
     2341 gggcgctgcg cgaccgcgag aaggtgacgc tcggcaacac ggaggtcgaa gcgatagcga
     2401 ctcccgggca cgcgctggcc caccactcct acctcgtcac cgatcaccgc cgtggggatg
     2461 agccgtggtt cgtcctcaca ggcgacgcgc tgctcgtcgg cgacgccggc cggccggacc
     2521 tccacgcgca cggggagcac accgccgagc agatggcgcg gagcctgtac cggtcgctga
     2581 ctgaacgcct actcgcgctc cccgacgacc tggtgcttta tccgtcgcac tactcgggtt
     2641 ccgtatgcgg gcgcgggctt tcgtcaaacc cgatttcgac catcggattc gagcgccgcc
     2701 acaatcccgc gcttcagctc gggtcggagg acgcctttgt cgaggcgctc ctgcgcgacg
     2761 taccgcccgc gccgcagcag caagccgcga tcgttgccgc caaccgcagc ggccggccgc
     2821 tcgccgaggt ggggtgaccg cctcaccggt tcgccttggg ctgcgtgaga acgcggccca
     2881 gttctcgctg ctcgtcgcca tcaacgtgtt cgtcggggcg atggtcgggc tcgagcgatc
     2941 gacgctgccg ctcgtagggc gcgacgactt cggcctgcgt tcgagcgcgg cggtcctgtc
     3001 cttcatcgtc gccttcggtg tggcaaaggc gttcacgaat ctcgccgccg gtgcgctcgc
     3061 ggagcgcgcg gggaggagga ggctgctggt cgcgggctgg gctgtcgccc taccggtgcc
     3121 gctgctgatc gccgtcgcgc cgagctgggg ctggatcgtg gcggcgaacg cgctgctcgg
     3181 tatcaaccag gggctcgctt ggtccatgac ggtggtgatg aagatcgacc tggtgggccc
     3241 ccgacgccgg ggcctcgcgc tcggcttgaa cgagtcggcc ggatacggcg gtgtggcgct
     3301 cgccgcgggg ctcggaggct ggctggcggg atcgtttgcc gcgcgggacg tgctcgtcgt
     3361 cgccggcgcg gcgatcgcga tcatcgcctt tctcctctcg ctcctgttcg tcaaggacac
     3421 gggggcgcac gttgagctcg agcagtcacg ccgcaccggt gatcgggacg tggctgcgcc
     3481 gccgcttcgc agcgctttcg cggctgcgac ctacggcgac cccaccttgc ggtcctgctc
     3541 tcaggcgggg ctgatcaaca acctcaacga tggcctcgcc tggggccttg tgccgctcta
     3601 cctggccgcc cacggccgca gcatcggaga gatcggcctg gtcgccgcgc tctatcccgg
     3661 cgtctggagc gtcgcgcaga tccccgccgg acactggtcg gacgtcatcg gtcgcaagcc
     3721 gctgatcgtc ggggggatgc tcgtccaggc ggtcgcgctc gcgctcctcg cgctcagcag
     3781 cggagccgtg cctgcggcag ccgtcgcagc cgtgctgctg ggtgcgggca cggcgctcgt
     3841 ctacccgact ttgatcgcgg ccatatccga cgcggtgagc ccggtggcgc gcgcgtccgt
     3901 ggtcggcgtt tatcgcttct ggcgcgacag cggatacgtg ttcggcggcc tgatcgcggg
     3961 tttggtcgcc gacgcacttg gttacggcgg cgctatctcg ctggtcgccg cgctcaccgc
     4021 cgcgtccggg ctgtgggtgc tccgcgacat gcggccttcc ggtactagcg cacgactatt
     4081 gtcacgcggc ccgactggta gccggactta gagccggtga agacgacact gcccttgcgc
     4141 gacgcgcgta gtttgaacgc caccgtcccg cgcttcccgg tccgctttgc ccgcttcgac
     4201 acgccgattc ccgagacgct cactcgcgcg ccgcggatag gccggccgcg aacattgagc
     4261 gccgtgattc ggacgacgct gctgcggcgc aggcggaggg aaccctcgat cttcaggttc
     4321 atacggcccg gcagcgtgag cgttcgtacc gtccacgcgc cgagggttcc gcgttcgtca
     4381 aggctcgcga cgcgccagta gagcgtgccg ccgtccttga agccgctctg cgtcagcagc
     4441 ggcgcgtagc tcgtgttctc cgtcacgtgg ctgtcgacga gcgtgctgaa gctgttggtc
     4501 ttcgagatct gcgcgcggta cgacctcgtg ccgatgttcg tgctcggatc ccagctgagc
     4561 agcacatgcc tcggcgtgtt ggtccaccgc acgccggtgg gcgcggcgat ctgacgtgtg
     4621 aacgtctgca gatccgagaa cggtcccgcc gtctcgcccg acgatgcctt cgggaagttc
     4681 gcccggacct gccagcgcac gttgccgact ccgaagaagc ccgtcggcgt gaacgccgtg
     4741 gagcgagtgt cgaagctctt ggcggtgccg tctccctgct cgacgtggag cgtgtaggag
     4801 gtcgctccag agaccgagtc ccagcgcacc accggaacgt cggggccgac ggtcggattg
     4861 tcggcgctga gcgacggggt gcgtagcgtg cggcggaacg tcgcgatgct cgaccagttg
     4921 agtgccacgt tgttctcgtc gttgacgcgg acacgccagt agagaatggt gtcggccggg
     4981 taggtgctcg tactcgtgta cgaggtggaa tccgtcgtga tgtcgtcgat cggatcgctg
     5041 aacgtgggat cgcccgatac ttgcagcctg taggtcttcg caccctgcgt cgcgggttgg
     5101 ctcgggaagt tcgcgttcgc caggctccag cggaacgtgg gctgctcgcc caccccggag
     5161 ccacccgtgg gtgagagcgg cgtgggcggg atcgagctct tctggaacct ttgcgggtgc
     5221 tccgcgccgg tgtcccaggt gcaggggcag agggtcccac tgccgccggc ggccggaagc
     5281 acctgccagt agtagtcggt gttctcgtct gaataggtcc gggccagtgt tcctcgcctc
     5341 ggcgcgtagg ccggcgcctt cgtgagggcg acatccacca cgttggtgaa ggaggagtcc
     5401 ttcgagacga ccacgtagta gctggaggca cccgcgatcg gcttccagcg gaataccggc
     5461 atgtcggtcg tgctggacgg cgttgtcatg gagaggtagt cgctctcctg cgcgtcgccg
     5521 agcggaccgg acccggtaac gggcgtgtaa gtgaaagcag gaccgaggct gccgccctgc
     5581 tgcgttgccc cgctgaccac gtccacgttc ttgaggtcct tgtcggtacg ggccatgacc
     5641 cggacgcact tgtcgccgct tccgagcccc gagttcgtgg agaccccagg ccacgagagc
     5701 gggcccgggt tcgtcgatga gctgtccgcg agtggcgtcc acgccgtcgt cgcggtgatg
     5761 tacgtcttcg tggcgctgct gccgaagtca cagttgctga tcacgctgtt ccacggcgcc
     5821 acatcgagtt cgtagctgga ggcgccggga accgggtccc acgtcaggag cggcgcagcg
     5881 gtcgacgctc cccaagcaag cggggtgttc gtgttgtcac gcagcgtcag gttcgggatc
     5941 gtcggcgtca catcgtcgaa ggccttgcgg aagtccggcc ccacgttcca gccaccggcg
     6001 ttcccgttca ggtcgatcgc gcgcacgcgc cagtgataga cgttgttcgc cagaaagcga
     6061 gtgggcgaaa gggatgtgcc ggtggtcagc tcgttgcagc acaccttgga gcccggcggg
     6121 aagtcgctga aggtattgac ctcgacttcg tagcgtgcgg cgccgggcac gacaccccac
     6181 gacaggttgg gtgagagcac cttcggccgg ccggcgccgt cgctttcatg ggaacccgtt
     6241 gcgtcttcga ccgtggtcga ggttccggtg ccccagctcc agtcgaagct tcccacggcg
     6301 gagggctccc cgtcgtgccc acccgtgtcc tcaggcgtaa tgccccagta gtaacgcccc
     6361 ggagcgagag atgccggcac tgcgaagctc gtacccgagg tctcgacggg ctttccgccg
     6421 gtccgcgaca cgacgagcga cccgagcgcc gggtcggtcg cgacgtagac caggtacttc
     6481 gacgcgcgag gcacggccga ccagtgcagc accagcgggg tatcgggata cgagatcgtc
     6541 gcgttgtccg ctggcgagag aagatgcgga gcgtcggtcc agctcttcgt gtacgcgcgg
     6601 ctccgcgacc actcgcccgc gacatctttg gagctgatcg aacgcacccg ccagtagtac
     6661 gtgccgtccg tgaggctctt gtcgatcgtg gcgaaggtgt tgagcgtctg cgtggccttg
     6721 atggcggagt tgaaggtgcg gtccgcggag agctggaact cgtacttggc cgcgctgcga
     6781 acggctcccc aggagaaagc gggcatcgcc gggccctgcg caccgtcccc cggcgccagc
     6841 ggccgcggcg gggcgagcgt gcggctcttg gcagcgtccg ctcgctggtc agcggccgca
     6901 tggcgtccga tctgcgggag caccgccgct ccggcgctcg cgctggcaag gagaaggccg
     6961 gcgccgaccg ccagtcctcg ccagcctacg cggcttcgtc tagcaggacc cttccgaagc
     7021 atgcgtctca ctatcacccg gcccctgtcc atcagccaga cgccgaaggt ctagtccagc
     7081 ctggacgtag gtctagcggg cggctcaccc gatgcggcat cgccgttacc acgaacgtgg
     7141 cggcaagcag ggcgatcgcc agaagcagcc ggccgagtga tggcgcgatg ccaacctggg
     7201 ccccggcctg gtcacccgag gccacgaaga cagcgtgtcc cggcagccag atgacgatcc
     7261 agaacagcgc gggcagagcc cacaggcgcg agagccgggg cgccgcgagc gcgactggca
     7321 cgaggaggag agcgaagtag tgaagccaca ccacggggga gatcactagc gcggccgcga
     7381 caaccgccga gaacgagcgg cgatcgccgt gctcgccccg c
//
LOCUS       DASVXS010000217         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_203033, whole genome shotgun sequence.
ACCESSION   DASVXS010000217 DASVXS010000000
VERSION     DASVXS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203033"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF752_12060"
     CDS             complement(<1..448)
                     /locus_tag="VF752_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8856322.1"
                     /translation="MADPASITWPGHATVLIELDGMRILTDPVLRRRIGPLTRIGPPV
                     PADAGERIDLVLLSHLHADHADLPSLRRLAGSAHLVVPRGSGPWLRTKGLTNVREIGV
                     GEEFDFGDFRLSTTRAEHDGRRRPMGGGAAAEALGYVVRGSRSLYFA"
     gene            506..1768
                     /locus_tag="VF752_12065"
     CDS             506..1768
                     /locus_tag="VF752_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856323.1"
                     /translation="MQGSEARALGRLAGRALGRPAGLARDVHGAVASRTFRALGILGA
                     PTRLMHDGISAAAYRTVGAALEAPLRAGGAALARTRAANGVPLADSLAGAFALGALNG
                     VYGDLLAREHRDLALELSVRQRGCDVVTDSAGLAEAFPDATPKLTIFVHGLCETEDAW
                     HVLPLARAEHERRSYGSRLREQLGYTPVYVRYNTGLHVSDNGRRLAQVIEHVASAWPA
                     DVEEIVLVGHSMGGLVARSACHYGEAESYGWIDHLRHVFCLGTPHLGAPLERAANVAG
                     WALGRLPETRPFADLVNARSAGIKDLRYGNCVEEDWCDCDPDEFLRDRCREVPFLPTA
                     TYCFIGATVTRDKDTRLASVVGDLLVQFPSASGQGKRRRIPFEIDNGLHLGGVNHFQL
                     LNHPAVYEQLRAWLARRPARPELSRTAT"
     gene            complement(1786..>2785)
                     /gene="serS"
                     /locus_tag="VF752_12070"
     CDS             complement(1786..>2785)
                     /gene="serS"
                     /locus_tag="VF752_12070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX8856324.1"
                     /translation="PIADEVREADERVRSLLVSLPNLPDPSTPEKEPEVLREVGSPGK
                     EGRDHLELLGDLVDMEAGARVSGSRFAYLKGDVVLLEFALVRWALDVLGDRGFTPVVP
                     PVLVREEALYGTGFLPDTEEQIYRVCDDDLYLVGTSEVPLASLHAGEILDEGALPIRY
                     AGFSTCFRREAGAAGKDTRGIFRVHQFDKVEMFAFVEPHASPDEHERLLSIEEDLLQA
                     LEIPYRVVNIPAWDLGASAAKKYDLEAWLPGQGRFRELTSCSNTTDYQARRLGVRYRP
                     DGGKPAEVHTLNGTAVAVGRTIIAVVENHQQDDGTISVPAVLHQYGAPATIAAAAD"
BASE COUNT          392 a         1004 c          996 g          393 t
ORIGIN      
        1 cggcgaagta cagcgagcgc gagcctcgca ccacgtagcc gagcgcttcg gcggcggcgc
       61 cgccccccat cgggcgcctg cggccgtcgt gctcggcgcg tgttgtggat agccggaagt
      121 cgccgaaatc gaactcctcg ccgacaccga tctctcgcac gttggtgagc cccttggtcc
      181 tgagccacgg gcccgaaccg cgcggtacga ccagatgcgc ggagccggcc agtcgccgga
      241 gcgacggcag gtccgcgtgg tccgcatgca ggtgggagag cagcaccagg tcgatccgct
      301 cgccggcgtc ggcaggtacc ggcgggccga ttcgggtgag aggtccgatg cggcggcgga
      361 ggaccgggtc ggtgaggatg cgcatcccgt cgagctcaat cagcacggtg gcgtgcccgg
      421 gccaggtgat cgaagcgggg tcggccatca tcgtcgatga tcgctgatcc ttgcgtccgg
      481 ccggcatcct tgttacaacg gccacgtgca gggcagcgag gcgagggcgc tgggacggct
      541 cgccgggcgg gctttgggga ggccggccgg gctggccagg gacgtgcacg gagccgtcgc
      601 gagccgcacg ttccgtgcgc tcgggatcct gggcgcgccc acgcggctga tgcacgacgg
      661 catctcggct gccgcttacc gcacggtcgg cgccgcgctc gaggcgccgc tccgcgccgg
      721 aggcgccgcg cttgcgcgca cgcgcgcagc gaacggggtg ccactggccg actcgctcgc
      781 gggcgctttc gcgctcggcg cactcaacgg cgtctacgga gacctgctcg cccgtgagca
      841 ccgcgatctc gcgctcgagc tgagcgtgcg acagcgggga tgcgacgtgg tgacggattc
      901 cgccgggctg gcggaggcct tccccgacgc gaccccgaag ctcaccatct tcgtccacgg
      961 gctctgcgag acggaggacg cctggcacgt gctcccgctg gcgcgcgccg agcacgagcg
     1021 ccggagctac ggctcgcggc tgcgcgagca gctcggctac acgcccgtct acgtgcgcta
     1081 caacaccggc ctgcacgtgt ccgacaacgg ccgccggctc gcccaggtga tcgagcacgt
     1141 ggcgagcgcc tggcccgcgg acgtcgagga gatcgtgcta gtcggtcact ccatgggcgg
     1201 cctcgtggcg cggagcgcct gccactacgg cgaggcggag agctacggct ggatcgatca
     1261 cctgcgtcac gtgttctgcc tcggcacccc acatctcggc gcgccgctcg agcgcgccgc
     1321 gaacgtggca gggtgggcgc tcggccggct gcccgagacg aggccgttcg cagacttggt
     1381 gaatgcgcgc agcgccggca tcaaggacct ccgctacggc aactgcgtcg aggaggactg
     1441 gtgcgactgc gatcccgacg agttcctgcg ggaccgctgc cgcgaggtgc cgttcctgcc
     1501 gaccgcgacc tactgcttca tcggcgcgac ggtcacccgg gacaaggaca cgcgactcgc
     1561 ctcggtggtg ggcgacctgc tcgtgcagtt cccgagcgcc tcgggccagg gaaagcggcg
     1621 ccgcatcccc ttcgagatcg acaacggcct ccacctcggc ggggtgaacc acttccagct
     1681 gctgaaccac cccgccgtgt acgagcagct ccgcgcctgg ctcgcgcgcc ggccggcccg
     1741 cccggagctg agccgtacag cgacctgagc ccccttggtg gtctctcagt ccgcggctgc
     1801 ggcgatcgtc gccggagcgc cgtactggtg gagcaccgcc ggcacgctga tcgtgccgtc
     1861 gtcctgctga tggttctcca cgaccgcgat gatcgtgcgc ccgacggcca ccgcggtgcc
     1921 gttcagcgtg tgcacctcgg ccggcttgcc gccgtcgggc cggtagcgca ctcccagccg
     1981 ccgcgcctgg tagtcggtcg tgttcgagca ggaggtgagc tcgcggaagc ggccctggcc
     2041 cggcagccac gcctcgaggt cgtacttctt cgccgccgac gcaccgaggt cccaggccgg
     2101 gatgttcacc acccggtacg ggatttcgag cgcctgcagg aggtcctcct cgatcgagag
     2161 cagacgctcg tgctcgtcgg gcgacgcgtg cggctcgacg aaggcgaaca tctcgacctt
     2221 gtcgaactgg tgcacgcgga agatcccgcg cgtgtccttg cccgcagcgc cggcctcgcg
     2281 gcggaagcag gtcgagaagc ccgcatagcg gatcggcagc gcgccctcgt cgaggatctc
     2341 gcccgcgtgc agcgacgcga gcggcacctc ggaagtgccg acgaggtaga ggtcgtcatc
     2401 gcacacgcgg tagatctgct cctccgtgtc cggcaggaag cctgtgccgt acagcgcctc
     2461 ctcgcgcacc agcacgggcg gcaccacggg cgtgaagccc cggtcgccga gcacgtcgag
     2521 cgcccagcgc accagggcga actcgagcag cacgacgtcg cccttcaggt aggcgaagcg
     2581 cgagcccgac acgcgagcgc cggcctccat gtccacgagg tcgccgagca gctccaggtg
     2641 gtcgcgcccc tccttgcccg ggctgcccac ctcccgcagg acctcgggct ccttctccgg
     2701 agtgctcgga tcgggcaggt tcggcagcga gacgagcagc gagcgcacgc gctcgtccgc
     2761 ctcgcgcacc tcgtcggcaa tgggg
//
LOCUS       DASVXS010000218        14919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_59661, whole genome shotgun sequence.
ACCESSION   DASVXS010000218 DASVXS010000000
VERSION     DASVXS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14919
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59661"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /gene="argS"
                     /locus_tag="VF752_12075"
     CDS             <1..1145
                     /gene="argS"
                     /locus_tag="VF752_12075"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8856325.1"
                     /translation="EREFYVNDYGSQIRRFGESIKARARGEEPPEDGYKGEYVKELAA
                     RIPGAADANPEELAEKGIELMLESVSDTLERFRVHFDRFFSERSLHGQNAVQHALDVL
                     EEGEHLYPHEGAVWLRSTTFGDDKDRVLMRSTGDWTYLAADIAYHQHKLERNYDRAID
                     VLGADHHGYIGRIKAAWQALGGDPDRFEIVIMQLVNLTEHGQRTQMSKRGGTIVTLDD
                     LIDDIGVDAARYFLLERSHDTPLDLDLARAREQSQENPVYYVQYAHARIASILRKEGD
                     ERVQAALEADYSEQLHPSERALLKRLLDLPEEVEQAAYRRAPHRLTAYVHEVAQQFSA
                     FYRDCRVIGAAEEGGDEDFRIALSVLAQRVIARSLDLLGVEAPHHM"
     gene            1380..2825
                     /locus_tag="VF752_12080"
     CDS             1380..2825
                     /locus_tag="VF752_12080"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8856326.1"
                     /translation="MRRIPPFARLLILAALAFAAIPAANASAGRLIVTGHDAEFHCTG
                     MQQCHYVLTAMNWARSGAPDPTKPVLVLDRNAEGEDSPGDPLDFPTALDNVDTLFGTG
                     PIPRVVMDPRSPEFATAPLTTDLYSAILVASDITCGGCDLNTDSSDPTSTPDSDAIFA
                     RRDAIEAFFNAGGGIYANAGADHADGNPSNGPDTYYNFLPIPATGVAVSAPFCLTAEG
                     IALGLEDQSCPDAGLHNGTHNDINCCATHNSFAEPPAGSPLRVAERDSEGFAETLIAE
                     GKIFHGTFDSTAPASAATASACSPTGFQPVTVTDESGGSGAKAVHFKVDGGAEQVAAV
                     DSSGKATLTIANGKHTVEFWGEDNAGNVEAAHHTVTVLTDTATKCAPKLAVAGVRRAC
                     VSSVVSVKIRATSASGIRSVTVSLDGKRLTTTKKSTFTVKVNARKLKAGRHKLTTVVT
                     SANGQKVTSSRTIARCAAAKPKRQTQPRFTG"
     gene            3117..5222
                     /locus_tag="VF752_12085"
     CDS             3117..5222
                     /locus_tag="VF752_12085"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8856327.1"
                     /translation="MNERRHSGGGRGILVALVALVAAVAWAIPAQANVTLPADNGKIA
                     FASDRDAPAEPGPAPTFRAGDDTCYTPSPETFGGSDSPPIYEDNNCWFDVFTTNPDGS
                     GVTNLTASNGGDHAYDDDPSWSPDGTKIAFASDRACAAAKAAGTNEEYCSASIYVMDA
                     NGSNVRRITAPDASQGEDDSNPTWSPNGGQIAFTRQGFERSRSGIWVVDASTSSDGRS
                     DAASRLSFEGSDDPDAVFSLDADPAWSPDGKSIAFTRFFVGGDVPLSVGRALHAYSAD
                     RNAEALAYARKKLAAIAPQGVPTPELLSITSTIARGQLDTGVVTQVTNPDACPVFIFI
                     PFPTSGSCQFDNHAAWTPDGNRVGFDREDVSFDGQIIGTDNPSPQLLPWGLNDNINIW
                     DASASGGDERSFAPDPAKDCASDSEVPVPGLIPTSQVCHAEMKPAFSPDGTRFAFHSD
                     SPDSNCYGMFFFLGDLNMCNHDVFTQSASDGSALEGGLAADRHDDRNADWQPVYKAPT
                     TAASVPACQTNGTVTVNASANAGSYGAPKAVHYKVDGGAEQTAPVSDGNAAINVPSGS
                     HTLEYWGENQAGAQEAAHHTATVKVDEVNNCAGDPTLSVAGVRRACVSSNLTVRVAVK
                     TAGKVKQVRVYLDGKRIRTLKKSKFSLRINARKLKAGRHRLTVIAVDVAGHSVRSTRT
                     IARCAQAKPKRKAAPRFTG"
     gene            complement(5305..6015)
                     /locus_tag="VF752_12090"
     CDS             complement(5305..6015)
                     /locus_tag="VF752_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8856328.1"
                     /translation="MRLTPALRRFISPRAAAVLLAAPAALLLTAAPASADGCSGADAQ
                     PTSSNLAQIESTTLCLLNAERRKRGLSSLRTNARLRLAALRHSRDMVARHYFAHESLG
                     GGDFEDRIRRAGYIPRYGAWAIGENIGWGTGILSTPGQMVNAWMNSPPHRRNILASDF
                     REIGIALAAGNPSPDAGDGGGTYTTDFGRTSRSVDSARRATRHRSARGKRAHIRRHKS
                     SRTSGCHATTNAAVSICP"
     regulatory      complement(6067..6141)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6309..7004
                     /locus_tag="VF752_12095"
     CDS             6309..7004
                     /locus_tag="VF752_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8856329.1"
                     /translation="MTAALVLAGANTALADSQPQTIKLSDEQTLTRWAHAGALSFIRA
                     QPSRKSRRVGRLHYWTEDGYREIWLALASREDANGNEWVQVRIPGRPNGRTGWVPRES
                     LAAFHTVRTQLVIDRRRLRATLYRSGKRVWRSPVGVGKRGTPTPGGHFYIREKIKALQ
                     GGTIYGPLAFGTSAYSNKLTDWPGGGVIGVHGTNQPQLIPGRPSHGCIRVPNRNILRL
                     ARLMPLGTPVHIL"
     gene            7001..8215
                     /locus_tag="VF752_12100"
     CDS             7001..8215
                     /locus_tag="VF752_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEX8856330.1"
                     /translation="MSGRTRVTSVLAAAAAAVALGVAVVPQRAGAGAGELLPDLVQGV
                     PAQVNVAALSDSGGKHWYLGFRSSVWNAGDGPLLIHGRDRYLNGTTPYMVAEQIVHRD
                     SGPDTTYEGIGQLHYYYGGNHNHWHFEPFDRYELVRPSDGVSMAPDLKQGFCLGDRHQ
                     VAGSNPQVFDQTCRPGEPDAASVDEGMSVGWADDYLPQVEGQKIEITGIDPGVYWLVH
                     RVNGTRAIHETNYDNNAASVAVRISWPSGFGGSPTLAVLRSCPGADSCPLPPADASQP
                     TPQPVTTTAGNSDRTAPKLVLSGARVQRLSRTRTVYTYVHCDEDCTLSAVAEVRPLRA
                     ARPWRFRPSDVALPAGARVKLTVRLPAKAIAAARRHRGGRVSLQLIATDLAGNASQAR
                     RTLALRVPRRTA"
     gene            complement(8212..8964)
                     /locus_tag="VF752_12105"
     CDS             complement(8212..8964)
                     /locus_tag="VF752_12105"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX8856331.1"
                     /translation="MIAAGGSVNTTVVAAFAVGFISFISPCVLPLVPGYLSAISGVSF
                     AELERGERRAHVLAPAILFCLSFTVMFVALGMSATGLGHTLNEHRQVLRHVSGIVIAG
                     LGVFFIATLFIPAFNREWRPSALLSRASAGGPVIAGLAFAVAWLPCTGPTLGAILTAA
                     STEATVAKGGVLLAFYAAGLAVPFVLSAIAFSRVTGFFKFFRRHYTVITVVSGGILIA
                     MGVLLYTNELTVLNQKALSLLDDLGINVFSNV"
     gene            complement(8964..9419)
                     /locus_tag="VF752_12110"
     CDS             complement(8964..9419)
                     /locus_tag="VF752_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX8856332.1"
                     /translation="MRRLAALMLVLALLAPGAALAAQPRASLADIEDEVMCQVCGTPL
                     NVSDSLQAQRERAFIVRLIDQGKTKQQIKDALVAQYGDSVLAVPKKSGFDLTAYLVPI
                     LVFLGAAAVIALAVTRWRRAKAASAAAQDPTSTPPGDQRLRADLERYDL"
     gene            complement(9416..9970)
                     /locus_tag="VF752_12115"
     CDS             complement(9416..9970)
                     /locus_tag="VF752_12115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8856333.1"
                     /translation="MKRLLAPIPLAAIVVTAALIALLAYGLTTSGTDKSIDNAVASGK
                     RPNAPALTLPRLTGGGESSLAEYKGKVVLVNYWASWCPPCRTEAPLLERWHQKMTKAG
                     GTVLGIDVLDTTDDALAFARAKGLTYPMLRDGDGKTQGKFGLTGYPESFVIDRDGKIA
                     ALQRGAVDDKFMLEKVQPLLEAQQ"
     gene            10051..10123
                     /locus_tag="VF752_12120"
     tRNA            10051..10123
                     /locus_tag="VF752_12120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10084..10086,aa:Gly,seq:ccc)
     gene            complement(10125..10508)
                     /locus_tag="VF752_12125"
     CDS             complement(10125..10508)
                     /locus_tag="VF752_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX8856334.1"
                     /translation="MLSAGLMLRRLWAAIRFAAKEEGFVRVFGAAVILVILGTVTYST
                     SQGWNVADGFYFAVCTLTTSSISDPHLTITGEPIKLFTAFYVLTGIGILVELARQIGV
                     AFIAMREERVAKHRKPGGDSAPGGD"
     gene            complement(10542..11468)
                     /locus_tag="VF752_12130"
     CDS             complement(10542..11468)
                     /locus_tag="VF752_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8856335.1"
                     /translation="MPEPIILVHGGAGSPAGGRIRNEDEVRSALESAVVAGSEVIERG
                     GDALDAAQAAVEWMEDCELFNAGRGSVLNDFGEVEMDAALMDGRDKRVGAVAGLSGLR
                     NPIAIARAVMDHSPHVLLAGAGAERFALGHGFDRVPRHHHVTERQIERWSRERDGAPP
                     VEGADASLGTVGAVVLDAAGNVAAATSTGGLRGKHVGRVGDSPLIGSGTYADNPTCAV
                     SATGNGEVIIRAVAAHETAALIAYKGLGLAEACDVVVRERIGPLGGEAGLLALDASGE
                     IATPFNAGIFQRAGRRGTGRVATAISERELEL"
     gene            11599..11973
                     /locus_tag="VF752_12135"
     CDS             11599..11973
                     /locus_tag="VF752_12135"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX8856336.1"
                     /translation="MRRLIVDGMNVIGSRPDGWWRDREGAMRVLAEQLELHAERTGDQ
                     VAVVFDGRPHEVPGERVDVTFASTRGPNAADRDIAERVERDPDPASLAVVTSDQALAE
                     RVRAAGAQVIGAGSFRRELEQS"
     gene            complement(11970..12608)
                     /locus_tag="VF752_12140"
     CDS             complement(11970..12608)
                     /locus_tag="VF752_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX8856337.1"
                     /translation="MLLPLLLALAAAGAPTHGAHPAAAVNPPIVQKRIPFGAKRKAEM
                     RRYARRHYGIDSFSLTNPKVIVEHFTATNSFASAWNTFARDVPDVELHELPGVCSHFI
                     VDRDGTIYQLVSTRIMCRHTVGLNYTAIGIEHVGTSDASVMGDRAQLRSSLALTRWLQ
                     QHYGILTRNVIGHAESLSSPYHHEDVARLRNQTHGDMQHATMVKYRRKLRAG"
     gene            12677..13015
                     /locus_tag="VF752_12145"
     CDS             12677..13015
                     /locus_tag="VF752_12145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8856338.1"
                     /translation="MDTWDISSLDVQPHFPEVLRSDPEARVIAINLPAGEMLQEHKVH
                     ERAYVVVVDGEVELVEDGNSKSGGCGLVAHFKAGEPHEVRAKSDSRILLILAPWPGEG
                     HPSQREQPAT"
     gene            13054..13458
                     /locus_tag="VF752_12150"
     CDS             13054..13458
                     /locus_tag="VF752_12150"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX8856339.1"
                     /translation="MDPSRKRRIRLGVALTAAVLLAGALMYTSFGASTKAETPSKLLS
                     SAQPGRSYEVTGKVATGSVKRDGDTLRFAIRDRNGTAKIPVVYTGTVPDPFREGREVI
                     VSGRVKSGVLIGERDSLVTKCPSKFTKAPQKS"
     gene            13469..>14919
                     /gene="ccsA"
                     /locus_tag="VF752_12155"
     CDS             13469..>14919
                     /gene="ccsA"
                     /locus_tag="VF752_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX8856340.1"
                     /translation="MATIGTACLVVALLTAVYAVGASLYGARTGRREFVASSRHAFYC
                     IAGLAIAATALIESRYLASDFSFSLVAQNSSTDLPTFYKLTALWSSQAGSLLFWVLVL
                     SIFSSIVLRVTRRSHREIVPYATAVLGGIAGFFLILMLAYASPFDTLSNPPVEGNGLE
                     PLLRHPAMMFHPPMLYAGYVGFAIPFAFAIGALVTRRTGADWIRSSRRFALIAWTFLG
                     TGILLGALWSYSELGWGGYWAWDPVENAALMPWLTGTAFLHSIMVQEKRGMLRVWNMV
                     LIIATFVLSLLGTFLVRSGVLDSIHAFGASTLGVPFLTFIAIVLAGSVALLLTRLDDL
                     RSRARIDSLLSREAFFLLNNLALVGLCFVIFWGTWFPLISEAVTGNKASVGPPWFDRY
                     TMPLALLLVALAGIGPLLAWRRVTPSAAARAFRIPLAVSAAVLVVLLAFTNAADSVTS
                     LILFCLVAFVLSAVGQEFWRGASARRVMTREPR"
BASE COUNT         2402 a         5156 c         5147 g         2214 t
ORIGIN      
        1 tggagcgcga gttctacgtg aacgactacg gcagccagat ccgccgcttc ggcgagtcga
       61 tcaaggcgcg cgcccgaggc gaggagccgc ccgaggacgg ctacaagggc gagtacgtga
      121 aggagctcgc cgcgcgcatc ccaggcgcgg ccgacgccaa ccccgaggag ctcgccgaga
      181 aggggatcga gctgatgctc gagagcgtga gcgacaccct cgagcgcttc cgcgtgcact
      241 tcgaccgctt cttctcggag cgctcgctgc acggccagaa cgccgtgcag cacgcgctcg
      301 acgtgctcga ggagggcgag cacctctacc cgcacgaggg cgccgtctgg ctgcgcagca
      361 ccaccttcgg cgatgacaag gaccgagtgc tgatgcgctc gaccggtgac tggacctacc
      421 tcgccgccga catcgcctac caccagcaca agctcgagcg caactacgac cgcgccatcg
      481 acgtgctcgg cgcagaccac cacggctaca tcggacgcat caaggccgcc tggcaggcgc
      541 tcggcgggga cccggaccgg ttcgagatcg tgatcatgca gctggtcaac ctcaccgagc
      601 acggccagcg cacgcagatg tcgaagcgcg gcggcaccat cgtcacgctc gacgacctga
      661 tcgacgacat cggtgtcgac gccgcccgct acttcctgct cgagcgaagc cacgacaccc
      721 cgctcgacct cgacctggcg cgggcccgcg agcagagcca ggagaacccg gtctactacg
      781 tccagtacgc gcatgcccga atcgccagca tcctccgcaa ggagggggac gagcgcgtgc
      841 aggctgcctt ggaagccgac tacagcgagc agctccaccc atccgagcgc gcgctgctga
      901 agcggttgct ggacctgccc gaagaggtag agcaggccgc gtaccggcgc gcgccgcacc
      961 gcctaaccgc atacgtgcac gaggtcgccc agcagttttc cgccttctac agggattgcc
     1021 gggtcattgg agccgccgag gaaggcggcg acgaggactt ccgcatcgcg ctcagcgtgc
     1081 tcgcccagag ggtgatcgcg cgctccctcg acctactcgg ggtcgaggct ccccaccaca
     1141 tgtagacccc ttccgcgctt gggtgtgacc cacttgggca tgcggtccaa ttcggggaat
     1201 tgacggttcg ttcagaaccg tgttcggatg gtccccagcg agaggcgccc gacccattga
     1261 gggagggccc tagtgcacaa aaggatccgt ctggccaacg aaagcaggga agcgcttcgg
     1321 accgagacgt gcgcgactag gccttccgcg actcgcctca cccgcgaaag gagttccgca
     1381 tgagacggat cccgcccttc gctcggttgc tcatcctcgc cgccctggcg ttcgcagcga
     1441 taccggctgc caacgcatcc gcaggccggc tgatcgtgac cgggcatgac gccgaattcc
     1501 actgcacggg catgcagcag tgccactacg tgctgacggc aatgaactgg gcgcgctcag
     1561 gggcaccaga cccgacgaag ccggtgctgg tgctcgaccg caacgccgag ggagaggaca
     1621 gccccggcga cccgctcgac ttccccacgg cgctcgacaa cgtggacacg ttgttcggca
     1681 cggggccgat cccgcgcgtg gtgatggacc cgcgctcgcc ggagttcgcc acagctccgc
     1741 tgaccacgga tctctacagc gccatcctcg tcgcgtccga catcacctgc ggtgggtgcg
     1801 acctgaacac ggacagcagc gaccccacat ccacaccgga cagcgacgcc atatttgcga
     1861 ggcgggacgc catcgaggcg ttcttcaacg cgggcggcgg catctatgcc aatgccggcg
     1921 cggaccacgc ggacggcaac ccgtccaacg gcccggacac gtactacaac ttcctgccca
     1981 tccccgcgac gggtgtggca gtgagcgcgc cgttctgcct gacggccgaa ggcatcgcgc
     2041 tgggactcga ggaccagagc tgtcccgacg ccggcctgca caacggcaca cacaacgaca
     2101 tcaactgctg tgcgactcac aactcgttcg ccgagcctcc cgcggggagc ccgctgaggg
     2161 tcgccgagcg tgacagcgaa ggcttcgccg agacgctgat cgccgagggg aagatcttcc
     2221 acggcacgtt cgacagcaca gcgccagcct cggcggccac ggcctcggcc tgttcgccca
     2281 ccgggttcca acccgtcacc gtgacggacg aatccggcgg ctcgggcgcg aaggcggtgc
     2341 acttcaaggt cgacggcggg gccgagcagg tggccgcggt cgattcgagt ggcaaggcaa
     2401 ccctcaccat cgcgaacggc aagcacaccg ttgagttctg gggcgaggac aacgccggca
     2461 acgtcgaggc cgctcatcac acggtcacgg ttctcacgga caccgccacg aagtgcgctc
     2521 cgaagctcgc ggtcgcgggc gtgcggcgcg cgtgcgtgag cagcgtcgtg agcgtgaaga
     2581 tccgggcgac ctcggccagt ggcatccgca gcgtcaccgt gtcgctcgac ggcaagcgct
     2641 tgaccacgac caagaagagc accttcacgg tcaaggtcaa cgcgcgcaag ctcaaggccg
     2701 gcaggcacaa gctgacgacg gttgtgacgt cggcgaatgg ccagaaggtc acgagctcgc
     2761 gcaccatcgc ccgctgtgcg gctgccaagc cgaagcggca gacccagcct cgcttcacgg
     2821 gctgagtcga agcggagatc ccgctaggga agaagggccg ggacgtttca gtcccggccc
     2881 ttcgcatgtg gtcctccatc tgacttgaga acttgacggt cttctcgact catgttctat
     2941 tggcgcggcg gagaggtatc ggatcagtta tcgggagggc gacgctcaac taggcgacag
     3001 gcacggattg cctcggtcat ctgaccggcg cacctgagct ctggcagcac tcgccgggga
     3061 ctggccacaa agccccaagg ccaccacgga cggcaaacag gaggactcgc aaaaccatga
     3121 atgaacgacg ccacagcggc ggcggtcgcg gcatccttgt cgcgctcgtc gcactggttg
     3181 cggctgttgc ctgggcgatt cccgcgcagg cgaacgtgac gcttccggcg gacaacggca
     3241 agatcgcgtt cgcgtccgat cgcgatgcgc cggcggagcc gggccctgcg cccacattcc
     3301 gcgcgggaga tgacacctgc tacaccccgt ccccggagac gttcggcgga agcgacagcc
     3361 cgccgatcta cgaggacaac aactgctggt tcgacgtgtt cacgacgaac ccggacggca
     3421 gcggggtcac caacctcacc gcctcgaacg gcggcgacca cgcctacgac gacgacccct
     3481 cgtggtcgcc ggacggcacg aagatcgcgt ttgccagcga ccgcgcctgc gcggccgcga
     3541 aggcggcggg cacgaatgag gagtactgca gcgcgtcgat ctacgtgatg gacgcgaacg
     3601 gatcgaacgt ccgcaggatc acggcgcccg acgcaagtca gggcgaggac gactcgaacc
     3661 cgacgtggtc gccgaacggc ggtcagatcg cgttcacgcg tcagggcttc gaacgcagtc
     3721 gttccgggat ctgggtcgtc gatgcctcga cgagctcgga cggcaggagc gatgccgcaa
     3781 gccgcctgag cttcgagggg tccgatgatc cggacgccgt cttctcgctc gacgcagatc
     3841 cggcgtggtc gcccgacggc aagtcgatcg cattcacgcg cttcttcgtg ggcggcgacg
     3901 ttccgctcag cgtgggccgg gccctccacg cgtacagcgc cgaccgcaac gcggaggcac
     3961 tcgcgtacgc gcgcaagaag ctcgcggcga tcgcgccgca gggcgtcccc acgccggagc
     4021 tgctcagcat cacgtcgacg attgcgcgcg gtcagctcga cacgggcgtg gtcacgcagg
     4081 tcacgaaccc ggatgcctgc ccggtgttca tcttcatccc gttcccaacc tccggtagct
     4141 gccagttcga caaccacgct gcgtggacgc cggatggcaa ccgggtcggg ttcgaccggg
     4201 aggatgtctc gttcgacggg cagatcatcg ggacggacaa cccgtccccg cagctcctgc
     4261 cgtgggggct gaacgacaac atcaacatct gggacgcctc ggcctcgggc ggcgacgagc
     4321 ggtcgttcgc gccggacccc gccaaggact gtgcgtccga cagcgaggtt ccggtccccg
     4381 gcctgatccc cacgtcccag gtgtgtcatg ccgagatgaa gccggccttc tcgccggacg
     4441 ggacgcgttt cgcattccac agcgacagcc cggacagcaa ctgctacggg atgttcttct
     4501 tcctcggcga cctgaacatg tgcaatcacg acgtgttcac ccagtcggca agcgacggca
     4561 gcgcgctgga gggtggcctg gccgccgacc gacacgacga ccgcaatgcg gactggcagc
     4621 cggtctacaa ggcgcctacc acggcggcga gtgtcccggc gtgtcagacc aacggcacgg
     4681 tgaccgtgaa cgccagcgcg aacgccggca gctacggcgc tccgaaggcg gtgcactaca
     4741 aggtcgacgg tggcgccgag cagaccgctc cggtcagcga cggcaacgcc gcgatcaacg
     4801 tgccgtccgg ctctcacacc ctcgagtact ggggtgagaa ccaggccggc gcgcaggagg
     4861 cggcgcacca caccgcgacg gtgaaggtgg acgaggtgaa caactgcgct ggcgatccga
     4921 cgctgtcggt cgcgggcgtg cgccgtgcct gcgtctcgtc gaacctgacc gtccgcgtgg
     4981 cggtcaagac ggcgggcaag gtcaagcagg tgcgcgtcta cctcgacggg aagcgcatcc
     5041 gcacgctcaa gaagtcgaag ttctcgctgc ggatcaacgc tcgcaagctc aaggccggcc
     5101 gccatcgcct cacggtgatc gcggtggacg ttgcgggcca cagcgtacgc tccacgcgca
     5161 cgatcgcccg ctgcgcccag gcgaagccga agcgcaaggc cgcgccgcgg ttcacgggct
     5221 gaggttctcg cggagaaccg cctgaatgac gaggggccgg gacggtcgtc ccggcccctc
     5281 ggcgttcctg cggagagcgg cgccctacgg gcagatgctg acggccgcgt tcgtcgtggc
     5341 gtgacaaccg gaggtgcgcg acgacttgtg gcggcggatg tgagcgcgct tgccgcgcgc
     5401 cgacctgtga cgcgtcgcgc ggcgcgcgct gtccacgctg cgcgatgtgc ggccgaagtc
     5461 agtcgtgtag gtgccgccgc catcgccggc gtccggcgac ggattgccgg ccgcgagtgc
     5521 gatgccgatc tcgcggaagt ccgaggcgag gatgttgcgc cgatgcggcg ggctgttcat
     5581 ccaggcgttc accatctggc cgggggtgga gaggatgccg gtgccccagc cgatgttctc
     5641 gccgatcgcc caggctccgt agcgcgggat gtagccagcg cggcggatgc ggtcctcgaa
     5701 gtcgccgccg ccgagcgact cgtgcgcgaa gtagtggcgc gcaaccatgt cgcggctgtg
     5761 gcgcagcgcg gcgagccgca gccgagcgtt cgtgcggagt gacgagaggc cgcgcttgcg
     5821 ccgctcggcg ttcaacaggc agagggtggt cgactcgatc tgggcgaggt tcgagctcgt
     5881 gggctgcgcg tcggctcccg agcagccgtc ggccgaggca ggagcggcgg tgaggagaag
     5941 agccgccggc gcggccagca gcacagcggc ggcacgcggg gagatgaagc ggcgtagggc
     6001 gggggtgagg cgcaagaagt cgtctcctgg agcgatcggg tcgccgcctt tcccgaccgg
     6061 atctcctgcg gtagcccggc tggctcgatg agccccgtgg ctttgcgtcc cgccctcgcg
     6121 gacggtttgc ctttctcggg aggcgactgg ctgtctctac gacggtgcat cggcatctca
     6181 gcggtgcgct tgagatctgg cacgtgcgta tgcgcgccaa tcgatcatcc gtccaggggg
     6241 ataaccgcac ccactggccg cgcggagcgg atgcataatg gaccgcgatg cgccgtcttc
     6301 tggctctagt gacggcggca cttgtcctgg cgggggcgaa cacggcgctc gcggactcgc
     6361 agccgcagac catcaagctg tcggacgagc agacgctcac gcggtgggcg cacgcgggcg
     6421 cgctgtcgtt catccgcgcg cagccgagca ggaagtcgcg tcgcgtcggc cgcctccact
     6481 actggaccga ggacggctac cgcgagatct ggctcgcgct cgcgagccgg gaggacgcga
     6541 acggcaacga gtgggtgcag gtgcgcatcc cgggacgccc gaacggacgc accggatggg
     6601 tgccgcgcga gagcctcgcg gccttccaca cggtgcgcac gcagctcgtg atcgaccgcc
     6661 gccgcttgcg ggccacgctc taccggagcg gtaagcgtgt ctggcgctcg ccggtcgggg
     6721 tcggcaagcg gggcacgccg acgccgggcg gccacttcta catccgcgag aagatcaagg
     6781 cgttgcaggg cggcacgatc tacggcccgc tcgcgttcgg cacgagcgcc tactcgaaca
     6841 agctcacgga ctggcccggc ggaggtgtga tcggcgtgca cggcactaac cagccgcagc
     6901 tgatccccgg ccggccatcg cacggatgca tccgggtgcc caaccgcaac attctgcggc
     6961 tggcgcggct gatgccgctg ggcacgccgg tacacatact gtgagcggcc gcacgcgcgt
     7021 gacatcggtg ctggcggcgg ctgccgcggc ggtcgccctc ggcgtggcag tcgtgccgca
     7081 gcgagcgggc gccggggccg gcgagctgct gccggacctc gtccagggcg tgcccgcgca
     7141 ggtgaacgtc gcggctctgt ccgactcagg cgggaagcac tggtacctgg gcttcaggtc
     7201 gtcggtgtgg aacgcaggcg acgggccgct gctgatccac ggtcgcgacc gttacctgaa
     7261 cggaaccacg ccctacatgg tcgccgagca gatcgtgcat cgggactcag ggccggacac
     7321 cacttacgag ggcatcggcc agcttcacta ctactacggc ggcaaccaca accactggca
     7381 cttcgagccg ttcgaccgct acgagctcgt ccgaccgagc gacggcgtgt ccatggcccc
     7441 cgatctcaag cagggcttct gtctaggaga ccggcaccag gtagccggct cgaatccgca
     7501 ggtcttcgac cagacgtgcc gccccggcga gccggacgcg gcttccgtcg atgagggcat
     7561 gtcggtcggc tgggcggatg actacctgcc gcaggtggaa ggccagaaga tcgagatcac
     7621 ggggatcgat ccgggtgtct actggctcgt ccaccgtgtc aacgggacca gggcgatcca
     7681 cgagacgaac tacgacaaca acgctgcgtc ggtggcagtc cggatctcat ggccgagcgg
     7741 tttcggcggc tccccgaccc tcgcggtgct caggtcgtgc cctggagcgg acagctgccc
     7801 gcttcctccg gcggacgctt cgcagcccac tccgcagccc gtcacgacga cggccggcaa
     7861 ctccgaccgc acggcgccga agctcgtgct gagcggcgcc cgagtgcagc gtctgtcccg
     7921 gacccgcacg gtctacacat acgttcactg cgacgaggac tgcacgctct ccgcggtcgc
     7981 cgaagttcga ccgctacgag cggcgcgccc gtggagattc cgcccgagcg atgtggcgct
     8041 tccggccgga gcgcgcgtga agctcacggt gcggctgcct gcgaaggcga tcgcagcggc
     8101 gcgcaggcac cggggagggc gggtcagcct tcagctgatc gcgacggacc tcgcaggcaa
     8161 tgcgtcgcaa gcccgccgca cgctggctct gcgcgtcccg cgccgcacgg cctagacgtt
     8221 gctgaagacg ttgatcccga ggtcgtccag caggctgagc gccttttggt tcagcaccgt
     8281 gagctcgttc gtgtagagca gcacgcccat cgcgatcagg atcccgcccg agacgaccgt
     8341 gatcaccgtg tagtggcggc ggaaaaactt gaagaagccg gtcacgcgcg agaacgcgat
     8401 ggcgctcagc acgaacggca ccgccagtcc ggccgcgtag aaggcgagca gcacgccgcc
     8461 cttcgccacg gtggcctcgg tgctggcggc ggtgaggatc gctccgagcg tcggcccggt
     8521 gcacggcagc caggcgacgg cgaacgcgag cccggcgatc acggggccac ctgcggaagc
     8581 ccgcgacagc agcgcactcg gtcgccactc ccggttgaag gccgggatga acagcgtggc
     8641 gatgaagaac acgcccaacc ccgcgatcac gatgcccgag acgtggcgta gcacctgacg
     8701 gtgctcgttc agcgtgtggc cgaggccggt cgcgctcatg ccgagcgcca cgaacatcac
     8761 ggtgaaggag aggcagaaca ggatcgccgg cgcgagcacg tgcgcccggc gctcaccgcg
     8821 ctcgagctcg gcgaatgaga cgcccgagat tgccgacagg tagccgggca cgagcggcag
     8881 cacacacggc gagatgaagg agatgaagcc gaccgcgaac gcggccacga ctgtggtgtt
     8941 gaccgatccc cctgcggcga tcatcagagg tcgtagcgct ctaggtccgc gcggagccgc
     9001 tggtcgcccg gaggcgtcga ggtcgggtcc tgcgccgcgg ccgacgcggc cttggcgcgt
     9061 cgccagcgcg tgaccgccag cgcgatcacg gccgcggcgc cgaggaacac aaggatcgga
     9121 acgaggtacg ccgtgaggtc gaagccgctc ttcttcggca cggcgagcac gctgtcgccg
     9181 tactgcgcca cgagcgcgtc cttgatctgc tgcttcgtct tgccctggtc gatgagccgc
     9241 acgatgaagg cccgctcgcg ctgcgcctgg agcgagtcgg agacgttcag gggcgtgccg
     9301 cacacctggc acatcacctc gtcttcgatg tcggccaggc tcgcccgcgg ctgcgccgcg
     9361 agcgcggcgc cgggcgcgag cagcgccagc acgagcatca gggcggcgag ccgcctcatt
     9421 gctgggcctc caggagcggc tgcaccttct ccagcatgaa cttgtcgtcc accgctcccc
     9481 gctgcagggc ggcgatcttg ccgtcgcggt cgatcacgaa gctctcgggg tagccggtca
     9541 gcccgaactt gccctgggtc ttgccgtcgc cgtcacggag catcgggtag gtgaggccct
     9601 tcgcgcgcgc aaacgcgagc gcatcgtcgg tggtgtcgag aacgtcgatt ccgagcaccg
     9661 tcccgccggc cttcgtcatc ttctggtgcc agcgctcgag tagcggcgcc tcggtgcggc
     9721 agggtggaca ccaggaggcc cagtagttca cgagcacgac cttgcctttg tactccgcga
     9781 gcgaactctc gccgccgccg gtgagccgcg ggagcgtgag cgcgggtgcg ttcgggcgct
     9841 tgccagatgc gaccgcgttg tcgatgctct tgtccgtgcc gctggtcgtg aggccgtagg
     9901 cgagcagcgc gatgagcgcc gctgtaacga cgatcgctgc aaggggaatg ggcgcgagca
     9961 agcgcttcac ggggccgcca agaagggtag agcggcgcgc gctcagcggg cgcgacaggc
    10021 cccgcttgcc gggctagaat cgccgggcac gcggacgtag ctcagttggt agagcaccag
    10081 cttcccaagc tgggggtcgc gggttcgagg cccgtcgtcc gctctcagtc gccgcccggc
    10141 gcgctgtccc cgccgggctt ccggtgcttc gcaacccgct cctcgcgcat cgcgatgaac
    10201 gccacgccga tctggcgcgc gagctccacc aggatgccga tgcccgtgag cacgtagaac
    10261 gccgtgaaga gcttgatcgg ctcgccggtg atggtcagat gcgggtccga aatgctcgac
    10321 gtcgtgagcg tgcagaccgc gaagtagaag ccgtccgcga cgttccagcc ctggctcgtc
    10381 gagtaggtga ccgtcccgag gatgaccagg atgacggccg ccccgaagac gcgaacgaac
    10441 ccctcttcct tggccgcgaa ccggatcgca gcccagaggc ggcggagcat gagccccgcg
    10501 gagagcatcg ccgcagggtc gcaggggcgc cggatggctc gctagagctc gagctcgcgc
    10561 tcggagatcg cggtcgcgac ccggccggtc ccgcgccgcc cggcccgctg gaagatcccg
    10621 gcgttgaacg gcgtagctat ctcgcccgac gcgtcgagcg ccagcaggcc ggcctcgccg
    10681 cccagcgggc cgatccgctc gcgcacgacc acgtcgcatg cctcggccag cccgagcccc
    10741 ttgtacgcga tcagcgccgc ggtctcgtgc gccgccaccg cgcggatgat cacctcgccg
    10801 ttgccggtcg cggacacagc gcacgtgggg ttgtccgcat acgtgccgga gccgatcagc
    10861 ggcgagtcgc cgacgcggcc cacgtgcttc ccgcgcaggc ctccggtcga agtggcggcg
    10921 gcgacgttgc cggcggcatc gagtaccaca gccccgacgg tcccgagcga cgcgtcggcc
    10981 ccctcgacgg gcggcgcgcc gtcgcgctcg cggctccagc gctcgatctg gcgctcggtc
    11041 acatggtgat gccgcggcac acgatcgaac ccatggccga gcgcaaagcg ctccgcgccg
    11101 gcgcccgcga gcaggacgtg cggcgagtgg tccatcactg cccgagcgat cgcgatcggg
    11161 ttgcgaagac ctgacaggcc ggccacggcg cccacgcgct tgtcgcgtcc gtccatgagc
    11221 gcggcgtcca tctccacctc gccgaagtcg ttcagcaccg agccgcgccc cgcgttgaac
    11281 agctcgcagt cctccatcca ctcgacggcc gcctgcgccg cgtcgagcgc atcgccgccg
    11341 cgctcgatca cctcggagcc ggccacaaca gcggactcga gcgcgcttcg cacctcgtcc
    11401 tcgttgcgaa tacggccgcc cgcggggctg cccgcaccgc cgtgtacgag gatgatgggc
    11461 tccggcaccg cgcgtgagga tatgacgatg aggcggctgc gggcgaactt ctcgtaccgt
    11521 ggtgcttagt gccgcatatg gcggcaaaat cgaccacggt agagcggaaa ctcgcccgcc
    11581 tggccgcggg tgcccacgat gaggcggctg atcgttgacg ggatgaacgt gatcggctcg
    11641 cgccccgacg gctggtggcg cgatcgcgag ggcgccatgc gcgtgctcgc cgagcaactc
    11701 gagctccatg ccgagcgcac cggggaccag gtcgcggtgg tctttgacgg caggccgcac
    11761 gaggtcccgg gcgagcgcgt ggatgtcacg ttcgcctcca cgcgcggccc gaacgcggcc
    11821 gaccgcgaca tcgcggagcg agtcgaacgc gaccccgatc ccgcctcgct ggcggtggtc
    11881 acctccgacc aggcgctcgc cgagcgcgtc cgcgcagctg gagcgcaagt catcggcgcg
    11941 ggcagcttcc gccgcgaact cgaacagagc tagcccgcgc ggagcttccg ccggtacttc
    12001 accatcgtcg cgtgctgcat gtcgccgtgc gtctggttcc gcagccgcgc gacgtcctca
    12061 tggtggtagg gcgaagagag cgactccgca tgcccgatca cattgcgcgt gaggatgccg
    12121 tagtgctgct gcagccagcg ggtgagcgcg agcgacgagc gcagctgcgc gcggtcgccc
    12181 atcacggagg cgtcgctcgt gccgacgtgc tcgatgccga tcgccgtgta gttcagcccg
    12241 accgtgtgtc ggcacatgat ccgggtggag acgagctggt agatggtgcc gtcgcggtcg
    12301 acgatgaagt gcgagcagac gccgggcagc tcatgcagct cgacgtccgg gacgtcgcgg
    12361 gcgaaggtgt tccaggcgga cgcgaacgag ttcgtcgccg tgaagtgctc gacgatcacc
    12421 ttcggattcg tcagcgagaa gctgtcgatg ccgtaatggc gtcgcgcgta gcggcgcatc
    12481 tccgccttgc gcttcgcgcc aaacggaatg cgcttctgca cgatcggcgg gttgaccgcc
    12541 gccgcggggt gggcaccatg cgtgggcgcg ccggcggcgg cgagcgcgag cagaaggggg
    12601 aggagcaccc gcacagcgtg gcaggaacgc ctttcggcga tgattccgcc cgaacgagga
    12661 gtcgacgagg agaccaatgg acacctggga catcagttcg ctcgacgtac agccgcactt
    12721 ccccgaggtg ctgcgcagcg atccggaggc gcgcgtgatc gcgatcaacc tcccggcggg
    12781 cgagatgctc caggagcaca aggtgcacga gcgcgcctac gtggtcgtcg tcgacggcga
    12841 ggtcgagctg gtggaggacg ggaacagcaa gtccggcggc tgcgggctcg ttgcccactt
    12901 caaggcgggc gagccgcacg aggtgcgggc caagagcgac tcgcgcatcc tcctgatcct
    12961 ggctccatgg cccggcgagg gccatccgag ccagcgcgag cagcccgcca cttaaccggc
    13021 agttcagaga gcggctgaca taatcggatg ccgatggatc cctcgcgcaa gcgccggatc
    13081 cggctcggtg tcgccctcac cgcggcagtg ctcctggccg gcgcgctcat gtacaccagc
    13141 ttcggcgcct ccaccaaggc ggagacgccc tccaagctgc tgagctccgc tcagcccggt
    13201 cgctcctacg aggtcacggg caaggtggcg accggttccg tgaagcgcga cggcgacacc
    13261 ctgcgcttcg cgattcgcga ccgcaacggc accgccaaga tcccggtcgt ctacacgggc
    13321 acggtgccgg acccgttccg ggaggggcgc gaggtgatcg tctcgggacg cgtgaagagc
    13381 ggcgtgctga tcggcgagcg ggactcgctg gtgacgaagt gcccgtcgaa gttcacgaag
    13441 gccccgcaga agtcgtgacg ttgcgctgat ggccacaatc ggcaccgcct gcttggtcgt
    13501 cgctctcctg accgcggtgt acgcggtcgg cgcgtcgctg tacggagccc ggaccgggcg
    13561 ccgcgagttc gtggcctcct cccgccacgc cttctactgc atcgccggcc tggcgatcgc
    13621 tgccacggcg ctgatcgaat cgcgctacct ggcgtccgac ttctcgttct cgctggtcgc
    13681 gcagaactcg tccaccgacc tgccgacctt ttacaagctc acggcgctct ggtcgagcca
    13741 ggccggatcg ctgctgttct gggtgctcgt cctgtcgatc ttctcgagca tcgtgctgcg
    13801 cgtcacccgg cgcagccacc gcgagatcgt gccttacgcg acagcggtgc tgggcggcat
    13861 cgccggcttc ttcctgatcc tgatgctcgc gtacgcgagc ccgttcgaca cgctctcgaa
    13921 cccgccggtc gaaggcaacg gtctcgagcc gctgctgcgc cacccggcga tgatgttcca
    13981 cccgccgatg ctctacgcgg gctacgtggg cttcgcgatc ccgttcgcgt tcgcgatcgg
    14041 cgcgctggtc acgaggcgta ccggcgccga ctggatccgc tcctcgcgcc gcttcgcgct
    14101 gatcgcctgg accttcctcg gcaccggcat cctgctcggc gcgctgtggt cgtactcgga
    14161 gctcggctgg ggcggctact gggcgtggga tccggtcgag aacgccgcgt tgatgccgtg
    14221 gctcaccggc accgcgttcc tgcactcgat catggttcag gagaagcgcg ggatgctccg
    14281 cgtgtggaac atggtgctga tcatcgcgac gttcgtgctg tcgctgctcg gcacgttcct
    14341 ggtccgctcc ggcgtgctcg actcgatcca tgcgttcggc gcctccacgc tcggcgtgcc
    14401 attcctcacg ttcatcgcga tcgtgctcgc cggctcggtc gcgctgctgc tgacgcgcct
    14461 cgacgacctc aggtcgcgcg cgcggatcga ctcgctgctc tcgcgcgagg cgttcttcct
    14521 gctgaacaac ctcgcgctcg tcggcctctg cttcgtgatc ttctggggca cctggttccc
    14581 gctgatctcc gaggccgtca ccggcaacaa ggcgagcgtc ggtccgcctt ggttcgaccg
    14641 ctacacgatg ccgctggccc tcctgctcgt cgcgctcgca ggcatcgggc cgctgctcgc
    14701 ctggcggcgg gtcacgccga gcgcggccgc gcgtgccttc cggattccgc tcgcggtgag
    14761 cgccgcggtg ctcgtcgtgc tgctcgcgtt cacgaacgcg gccgacagcg tcacgtcgct
    14821 gatcctcttc tgcctcgtgg cgttcgtgct cagcgcggtc gggcaggaat tctggcgcgg
    14881 tgcatcggcg cggcgagtga tgaccaggga gccgcgcat
//
LOCUS       DASVXS010000219        12694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_595992, whole genome shotgun sequence.
ACCESSION   DASVXS010000219 DASVXS010000000
VERSION     DASVXS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12694
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595992"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..984
                     /locus_tag="VF752_12160"
     CDS             157..984
                     /locus_tag="VF752_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8856341.1"
                     /translation="MGVLDGKSAIVTGSARGIGRATAELLAEQGAQVLINDLDGDVAE
                     EASNEIQGETVAFAGDLTKPGVPDHLVQKAVDEFGQLDIVVNNAGYTWDGPIHKMSDE
                     QFQAMLDIHNVVPFRVLRAAAPHMREPAKQEREEGREVFRKVVNVSSISGTMGNAGQA
                     NYSSGKSGVVGLTKTLAKEWGQFKINVNAVAFGFVETRLTASKEEGGTFQKDGEEIKL
                     GIPEQMRQMAAMLIPLGRPATPAEAAGGIFFLCSPWSNYVHGQVLNVTGGQFTGMTS"
     gene            1031..2209
                     /locus_tag="VF752_12165"
     CDS             1031..2209
                     /locus_tag="VF752_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856342.1"
                     /translation="MAGVAAVLVVVLSSGDDGSGSPPDPRAKLPLIERRVEQIRALDF
                     KEPVPARLVTPAQVKAEGVAELNRMVPSQLIDTNEVLQFLGLLPEGTNLRRLLASALE
                     QQVAGLYDPRIHRLELVRRKGSQNATLDELTLAHELDHALDDQNFRLKDVNAVTDDAA
                     TAYSALVEGTATAVMQDYARQHVSGTDALGAALASAGAGSSEGLPRYVVDSLAFPYIA
                     GLKFVTRLRELGQGWKLVNFAFSARQPTTSEQVIHPLAYLRGAGAVPVGLPPAGALGR
                     GWQRDVAGSFGEFDTYELLRTGLSDARARRASEGWGGGRYALFRRGQYDPACKPPCRA
                     TEALAVRWRWDSQRDAREFAAALRVYAEHRRGAAVSTSPKETTLAIAPTSALARTLTR
                     "
     gene            2293..2775
                     /locus_tag="VF752_12170"
     CDS             2293..2775
                     /locus_tag="VF752_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8856343.1"
                     /translation="MSVRVQMTVNGESHASEVEPRLLLVHYLRDVLGLTGTHVGCDTT
                     NCGACTVHLDGDAVKSCTVLAVQADGCEVTTIEGLAHDGELHPLQDAFWADHALQCGF
                     CTPGIIMAAAAYLERNPDPSDDEIRHALEGNLCRCTGYQNIVKAVRTAAAASRKEVAA
                     "
     gene            2772..5246
                     /locus_tag="VF752_12175"
     CDS             2772..5246
                     /locus_tag="VF752_12175"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX8856344.1"
                     /translation="MTTVERPAEERGWVGQALRRKEDPPLITGMGRFVEDVVLPRTLW
                     MAVIRSPEAHARIVSIDASAARERPGVAAVFTGEDLADLGAPLPMIWAPPGVEMRTPE
                     HWPLARGAVKHVGDPVAVVIGEDRYAVVDAAEDVIVEYEPLPAVVDPEAALEEGSPLV
                     WEEFGTNVTHEWGLGSEDIEQALAEAEVVVERRIVNHRTAGVAIEPRGALADWREGEL
                     TLWSSTQIPHVTKFVLSGMLGIPEDRLRVIAPDVGGGFGSKLNVYAEEAIVAWCARRL
                     NRPVKWIESRTENLSATTHGRDQIDYVRMGGTRDGRITALHTKIVLDCGGYQQLLTPF
                     MGVFSACVMSGCYAIPKVRTDVIGVFTNKVATDAVRGAGRPEATHLIEVTVDQFAREI
                     GMDPLELRRKNFIPKENFPAEVATGLVYDSGDYHGTLDKCLEHVGLEQFRGEQEELRK
                     KGVYRGVGFSTYMEMCGLAPSRIVGPKGVGLQAGFWESATVRVHPSGSVTVLTGTSPH
                     GQGHETSFSQIAADLLGVSYDDVEVIHGDTNAGPHGMDTYGSRALAVGGEAVARAATK
                     VADKAKRIAAHLLEAAPGDIEIRDGRYQVRGSPEAGMPLSDICAAAYVPEDIPVDIEP
                     GLEETSFFDPENFVWPFGAHACVVEVDVETGEVDVVRWVAVDDCGPAINPTLIEGQVH
                     GGVVHAIGQALYEQIVYDSDGQLTTGTLVDYALPTAAEVPDIETDRTETPSPVNQLGV
                     KGVGEAGTIAATPAIVNAVIDALSPLGVTFINMPLTPMRVLEAIDEARGRGTGPRATE
                     QAREVGEHGEGAAGSGPAQPDEGGAP"
     gene            5243..6088
                     /locus_tag="VF752_12180"
     CDS             5243..6088
                     /locus_tag="VF752_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX8856345.1"
                     /translation="MIPPEFEYVAPESLDDAIRALSDGGADAKLLAGGHSLLPLMKVR
                     LAAPSLLVDLRRVPGLSGIEPHNGGFRIGAMTHHADVQFTPALGLASRAASLIADQQV
                     RNRGTIGGSLAHADPAADMPAVLLALEGEVTVRGPSGDRSIAAADLFRDFLTTSIEPD
                     EVLTEVLMPSSEGWGVGYQKFVRRAEDWATVGVCALVRANEGVCEDVRVGLTHMAGVP
                     LRATAVEAALRGQPLDSQHIAQAAENAAEGTDPPGDVNATPEYKRRLARVLCRRALEE
                     AAGTG"
     gene            6085..6669
                     /locus_tag="VF752_12185"
     CDS             6085..6669
                     /locus_tag="VF752_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8856346.1"
                     /translation="MSGTARATGGIVLAAGEGRRFGGRKQLAELHGRPLLEHVLSAMA
                     ATPLERVVVVLGANADEVLSGVDLYGAEPVVFAAWHEGQAASLRAGLDALGDVAGAVV
                     ALGDQPRLSPLAVERLLSAPTDGFDAARATYAGKPGHPVLLRRSLFPAVRQLSGDEGA
                     RGLLEHARVASVACDGLGGPEDVDTRDELEELNR"
     gene            6666..7142
                     /locus_tag="VF752_12190"
     CDS             6666..7142
                     /locus_tag="VF752_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8856347.1"
                     /translation="MKLAQAFEVTAPVERVWATLIDVEAVAPCLPGAEVTASDENGVY
                     HGTFQVKIGAATAAYRGTLSIDAVDEATRTVTLRANGQDKRGQGSARATIVSTLIPGE
                     AGATHVEVETDLTITGRLASFGRPGVMQDVANRLLRDFARCLEQRIGAGASAGADP"
     gene            complement(7218..8420)
                     /locus_tag="VF752_12195"
     CDS             complement(7218..8420)
                     /locus_tag="VF752_12195"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX8856348.1"
                     /translation="MRYELRGDVPKKRHTVFRDNGTLLTEEVMGFEGFSGNESILYHL
                     VSPCRVMRLGEFEPIVREEWVPDAHAHHHFATKGIEPEGDPIRGRRLLMWNGDVEIAI
                     CRPETGHDYFYRNGEGDEVVFVHEGRGELETVFGTLPYSEHDYIVIPRGTTYRFRPEG
                     EQLHLTFHTPGEIETPKRYRNRYGQLLEHAPYSHRDFRSPTELVTHRDTGEYELVVRV
                     RDGYQHYTLDYHPLDVVGWDGYVYPYTFNIDDFEPVTGRIHMPPPVHQTFEGPNFVIC
                     SFCPRMLDFDENAIPIPYHHSNLQSEEMIYYVSGEFGSRKGIDVGSITLHPSGLPHGP
                     QPGLAEKSIGMKHTNELAVMCDTFRPLRLTQLARELDDGRYALSWYENAEAGATDASS
                     PAGVTSHV"
     gene            complement(8417..9562)
                     /gene="hppD"
                     /locus_tag="VF752_12200"
     CDS             complement(8417..9562)
                     /gene="hppD"
                     /locus_tag="VF752_12200"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX8856349.1"
                     /translation="MAIEQLHGARPQTAPPAPADDRMPIHGIDHVELYVGNAAQAAYF
                     YVHGFGFTETAFRGLETGTRDRVSRVVEQGRIRLVLTGTLTGEDEIADHHRRHGDGVI
                     DIALSVPDAAAAYEWAVDHGARGVSTPHWLEDEHGRVQVASIATYGETRHTFVQRREY
                     DGPFLPGYEARAPRPKAGDGMFVGIDHIVGNVELGHMEEWVHYYERVFGMTEMIHFSD
                     EAISTEYSALMSKVVTDGHGRIKFPINEPATGKRKSQIEEYIDFYRGAGAQHIAIATT
                     DIVNTVAELTARGIEFLAIPGEYYDEVPDRIGEIDESLDDLRRLGILVDRDDEGYLLQ
                     IFTKPVGDRPTVFFEVIERHGARGFGEGNFKALFQAIEREQERRGNL"
     gene            9650..10099
                     /locus_tag="VF752_12205"
     CDS             9650..10099
                     /locus_tag="VF752_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8856350.1"
                     /translation="MATFQPQFDEVDRQIVALLRADARRSFQDIGKHVHLTAPAVKRR
                     VDRLERDRVIRGYTTVVDQEAFGWGSEAFVFLYCEGRMPASAIRDAVEKEPGVASAHT
                     VAGESSAILHVLARDTKELELTLERIRHNEGVTRTVSQVVLSTLFQR"
     gene            10096..10758
                     /locus_tag="VF752_12210"
     CDS             10096..10758
                     /locus_tag="VF752_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8856351.1"
                     /translation="MSRRAYPAGAQPPSVRDFYAFEEHVRNARRGRGLDVPTEWYEIP
                     VFYFSNPAAIYGDGDAVPMPADTAELDYELELACVIGPDGRPEGFTVMNDWSARDLQR
                     REMRVGLGPAKGKDFATSLGPELVSPDELPADLDMRAVARVNGEVRTDSRTGGMQFSW
                     DELLEAAARNTRGLQPGDVVGSGTVGAGCILEHGDGRWLERGDVVELEIEGIGVLRNT
                     VA"
     gene            complement(10762..11601)
                     /locus_tag="VF752_12215"
     CDS             complement(10762..11601)
                     /locus_tag="VF752_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8856352.1"
                     /translation="MPSQIFRPDLLDDKIVLLTGGGTGLGRATALELTALGARVVIAG
                     RRQEPLDETAAMCEGGRCEAIACDIREEDQVEALVDRVLQRHGRIDTLVNNAGGQYMT
                     PAEDISPKGFRTVVRVNLEGTWLMTHAVATKAMMPESRGGKIVNVTLSPHHGLPGMAH
                     SSAARAAVENMTRVLSIEWARFGIRLNAIASGHFETEAIEKYPEPVRAGVARTVPLQR
                     LGRPEEQAWLVAFLASPAGDYYSGSIITIDGARDNWFGPWPPPGLSDEAGKPLAEERR
                     PKT"
     gene            11669..12088
                     /locus_tag="VF752_12220"
     CDS             11669..12088
                     /locus_tag="VF752_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX8856353.1"
                     /translation="MEAATQTQPRVGQGALERLFKISERGSNVRIELLGGLTTFLTMS
                     YILFVNPAILSTKGTGMPFEAVAVATALAACIATMAMGLFTNYPFALASGLGLNAVVA
                     FDIILGRNLRWEVGMACIVIEGLLALVLVIAACAKPS"
     gene            12085..12663
                     /locus_tag="VF752_12225"
     CDS             12085..12663
                     /locus_tag="VF752_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX8856354.1"
                     /translation="MRAVPTSLKLSIGVGIGLFITLVGLRDGGIVVNDPATGIGLGDL
                     THGAPLIALGGILVAVLLSVRNVRGAILLGVLASTALGLIFGVLDGPSKIAEIPGSGS
                     FSTIGDAWKPDNLSDALQVALIPTIFALFMTDFFDTIGTVVAVGGSGGLLDRAGNLPG
                     IRRVLLVDSGAAALGGTFGPRLSRPMWRAARA"
BASE COUNT         1939 a         4306 c         4505 g         1944 t
ORIGIN      
        1 ccgatcggcg gcggcaccga cgagatcatg aaggagatcc tgggcaagca gatcgggctg
       61 tgaaaaggcc atgactagcc gccgccaccc ccagggccga acgccgagcg cgagcactag
      121 gctccccggc ctcacttcga ccaaggagac atacccgtgg gagtactcga cggcaaatcg
      181 gcgatcgtca ccggctcggc gcgcggcatc ggacgcgcga cggctgagct gctcgccgag
      241 cagggcgccc aggtcctgat caacgacctc gacggcgacg tggccgagga ggcctcgaac
      301 gagatccagg gcgagaccgt cgccttcgcc ggcgacctga ccaagcccgg cgtgcccgac
      361 cacctcgtgc agaaggcggt cgacgagttc ggccagctcg acatcgtggt gaacaacgcc
      421 ggctacacct gggacgggcc gatccacaag atgtccgacg agcagttcca ggccatgctc
      481 gacatccaca acgtggtgcc cttccgcgtg ctgcgggcgg ccgcgccgca catgcgcgag
      541 cccgccaagc aggagcgcga ggagggacgc gaggtcttcc gcaaggtcgt gaacgtgtcc
      601 tcgatctcgg gcacgatggg caacgcgggc caggcgaact actcctcggg caagtcgggc
      661 gtcgttggcc tcaccaagac gttggccaag gagtggggcc agttcaagat caacgtgaac
      721 gccgtcgcct tcggcttcgt cgagacccgg ctgaccgcgt ccaaggaaga gggcggcacc
      781 ttccagaagg acggcgagga gatcaagctc ggcatccccg agcagatgcg ccagatggcc
      841 gcgatgctga tcccgctcgg ccgcccggcc accccggcgg aggccgccgg aggcatcttc
      901 ttcctctgct cgccgtggtc gaactacgtg cacggccagg tcctcaacgt gaccggtggc
      961 cagttcaccg gcatgacttc gtagcgccac gacccggcgt cgactcatag cggccgcggc
     1021 gctcgccctc gtggcgggcg tcgcggccgt tctcgtggtg gtgctgtcct ccggcgacga
     1081 cggctcgggc tccccgcccg acccgcgggc gaagctgccg ctgatcgagc ggcgcgtcga
     1141 gcagatccgc gcgctcgact tcaaggagcc cgtccccgct cggctggtca cgcccgcgca
     1201 ggtgaaggcc gagggagtgg cggagctgaa ccggatggtg ccctcgcagc tgatcgacac
     1261 caacgaggtg ctccagttcc tcggcctgct gccggagggc acgaacctgc gccggctgct
     1321 cgcttcggcg ctcgaacagc aggtggccgg gctgtacgac ccgcggatcc accggctcga
     1381 gctcgtgcgc cgtaagggat cgcagaacgc gacgctcgac gagctgacgc tcgcgcacga
     1441 actcgatcac gcgctcgacg accagaactt ccgcctcaag gacgtgaacg ccgtgaccga
     1501 cgacgcggct acggcgtact ccgcactcgt cgaggggacg gcgacggcgg tgatgcagga
     1561 ctacgcacgg cagcacgtga gcggcaccga tgcgctcggg gcggcgctcg ccagcgcggg
     1621 cgccggctcc agcgagggcc tgccgcgcta cgtggtcgat tcgctcgcgt tcccctacat
     1681 cgcgggactc aagttcgtca cgcgtttgcg cgagctcggg cagggctgga agctcgtgaa
     1741 cttcgcgttc tcagcgcgcc agcccaccac gagcgagcag gtgatccacc cgctcgcgta
     1801 cctgcggggt gcgggcgccg tgccggtcgg gctgccgccc gcgggcgcgc tcggtcgcgg
     1861 ctggcagcgc gacgtcgccg gcagcttcgg cgagttcgac acgtacgagc ttctccgcac
     1921 gggtctgagc gatgcgcgtg cgcgccgggc ctccgagggc tggggcggcg gccgctacgc
     1981 gctgttcagg cgtgggcagt acgaccccgc ctgcaagccg ccatgccgag ccaccgaggc
     2041 gctcgccgtg cgctggcgct gggacagcca gcgcgacgcg cgcgagttcg cggccgcgct
     2101 gcgcgtgtac gccgagcacc gccgcggcgc ggcggtctcg acgagcccga aggagacgac
     2161 gttggctatc gcgccaacct ctgcactggc gcgcacgctc acacgctgag aagacgccca
     2221 agtccttccg acacccgccg ggcgggagta gcatcggttc gaagcgccgt tccactctcg
     2281 ggaggagaga ccatgagcgt tcgggtgcag atgaccgtca atggcgagtc acacgcgtcc
     2341 gaggtggagc cgcgcctgct tctcgtccac tatctgcgcg acgtgctcgg gctgaccggc
     2401 acgcacgtcg gctgcgacac gacgaactgc ggcgcgtgca cggttcacct cgacggcgac
     2461 gcggtcaaga gctgcacggt tctcgcggtc caagccgacg gctgcgaggt gaccacgatc
     2521 gaggggctgg cgcacgatgg cgagcttcat cccctccagg acgccttctg ggcggatcac
     2581 gcgctgcagt gcgggttctg cacgccgggg atcatcatgg cggctgccgc gtacctcgag
     2641 cgcaacccgg acccgtccga cgacgagatc aggcacgcgc tggagggcaa cctctgccgt
     2701 tgcaccggct atcagaacat cgtgaaggct gtgcgcaccg cggccgccgc gtcacggaag
     2761 gaggtggcgg catgaccacc gtggagcgac ccgctgaaga gaggggctgg gtggggcagg
     2821 cgctgcgccg caaggaggac ccaccgctca tcacgggcat ggggcgattc gtggaggacg
     2881 tcgtcctgcc ccgcacgctg tggatggcgg tcatacgctc gcccgaggca cacgcgcgca
     2941 tcgtctcgat cgacgcctct gccgcacgcg agcgaccggg cgtggccgcc gtcttcacgg
     3001 gcgaggacct ggccgatctc ggcgcgcccc tcccgatgat ctgggcgccc cccggcgtgg
     3061 agatgcgcac tcccgagcac tggccgctgg cccgcggtgc ggtgaagcat gttggcgatc
     3121 ccgtcgccgt cgtgatcggc gaggaccgct acgccgtcgt cgacgcggcc gaggacgtga
     3181 tcgtggagta cgagccgctg ccggcggtgg tcgatcccga ggctgccctg gaagagggct
     3241 cgccgctggt gtgggaggag ttcgggacga acgtgaccca cgagtggggc ctcggctccg
     3301 aagacatcga gcaggcgctt gcggaggcgg aggtggtggt cgagaggcgg atcgtgaacc
     3361 accgcaccgc cggggtcgca atcgagccac gcggggcgct cgcggactgg cgcgaaggcg
     3421 agctgacgct gtggagctcg acgcagatcc cgcacgtcac gaagttcgtg ctctcgggca
     3481 tgctcggcat acccgaggac cggctgcgcg tgatcgcccc ggacgtgggc ggcgggttcg
     3541 gctcgaagct gaacgtgtac gccgaggagg cgatcgtcgc ttggtgcgcc cgcaggttga
     3601 accggccggt gaagtggatc gagagccgca cggagaacct gagcgccacc actcacggcc
     3661 gcgaccagat cgactacgtg cggatgggtg ggacccgcga cggcaggatc accgcgctgc
     3721 acacgaagat cgtgctggat tgcggcggct accagcagct gctcacgccg ttcatgggcg
     3781 tgttcagcgc gtgcgtgatg agcggctgct acgcgattcc aaaggtgcgg accgatgtca
     3841 tcggcgtgtt caccaacaag gtcgccaccg acgctgttcg cggagcaggc aggcccgagg
     3901 cgacccacct gatcgaggtg accgtcgacc agttcgcccg ggaaatcgga atggacccgc
     3961 tcgagcttcg aaggaagaac ttcatcccga aggagaactt cccggccgag gtggcgaccg
     4021 gcctcgtgta cgactccggc gactaccacg gaacgctcga caagtgcctc gagcatgtgg
     4081 gcctcgagca gttccgcggc gagcaggaag agctccgcaa gaagggcgtc tatcgaggcg
     4141 tcgggttctc cacctacatg gagatgtgcg gcctcgcgcc ttcccggatc gtcggtccca
     4201 agggcgtcgg cctgcaggcc ggattctggg agtcagcgac ggtacgggta catccctcgg
     4261 gctcggtgac cgtgctcaca gggacttctc cgcatgggca gggccacgaa acgagcttct
     4321 ctcagatcgc ggccgatctg ctcggcgtgt cgtacgacga cgtcgaagtg atccacggcg
     4381 acaccaatgc cggcccgcac gggatggaca cgtacggctc ccgggccctg gcggtgggcg
     4441 gagaggcggt cgcgcgcgcc gccaccaagg tggccgacaa ggccaagcgc atcgcggcac
     4501 atctgctcga ggccgcaccg ggggacatcg agatccgcga cggccgctac caggtgcggg
     4561 gctcgccgga ggccggcatg ccgctctccg acatatgcgc ggccgcatac gtgcccgaag
     4621 acatcccggt cgacatcgag ccagggctcg aggagacgag cttcttcgac ccggagaact
     4681 tcgtatggcc gttcggagcc cacgcgtgcg tggtcgaggt ggacgtggag accggcgagg
     4741 tggacgtcgt gcgctgggtg gcggtcgatg actgcggccc cgcgatcaac cccactctga
     4801 tcgagggcca ggtgcacggc ggcgtggtgc atgcgatcgg gcaggcgctc tacgagcaga
     4861 tcgtctacga ctcggacggc cagttgacga cgggcacgct ggtggactac gcgctgccca
     4921 ccgcggccga ggttccggac atcgagacgg accgcaccga gacgccctcg cccgtcaatc
     4981 agctcggcgt gaagggcgtg ggcgaggcgg gcacgatcgc ggcgaccccc gcgatcgtga
     5041 atgcggtgat cgacgcgctc agcccactgg gcgtcacgtt catcaacatg ccgctcacgc
     5101 cgatgcgcgt tctcgaagcg atcgacgagg cgcgcggccg gggcaccggt ccacgtgcca
     5161 ccgagcaggc tcgcgaggtt ggcgaacacg gcgaaggggc ggccggctca ggaccagctc
     5221 agccggacga gggaggtgct ccgtgattcc ccccgagttc gaatatgtcg cgcctgagtc
     5281 gctcgacgat gcgatccggg cactgtccga cggcggcgcc gacgcgaagt tgctcgccgg
     5341 cggacactcg ctgctcccgc tgatgaaggt gcgcctggcc gcgccctcgc tgctcgtgga
     5401 cctgcgccgt gtgccgggtc tcagcggaat cgaaccccac aacggtggct tccgaatcgg
     5461 cgccatgacc caccacgccg acgtgcagtt cacgccggca ctcggcctcg cgagcagagc
     5521 ggcgagcctg atcgccgacc agcaggtgcg caaccgcggc acgatcggcg gctccctcgc
     5581 gcacgctgac cccgcggccg acatgcccgc ggtgctgctg gcactcgagg gcgaggtcac
     5641 ggtgcgcggg ccgagcggcg accggagcat cgcggcggcc gacctcttcc gggacttcct
     5701 cacgacgtcg atcgagccgg acgaggtcct gaccgaggtg ctgatgccct cgtccgaggg
     5761 ctggggagtc ggctaccaga agttcgtccg ccgagccgag gactgggcga cggtcggggt
     5821 ctgcgcgctc gtgcgtgcga acgagggggt gtgcgaggac gtgcgcgtgg gactgacgca
     5881 catggccggt gtgccgctcc gggcgacggc cgttgaggcg gcattgcgcg ggcaaccgct
     5941 cgactcgcag cacatcgcgc aggcggccga aaatgctgcc gagggcaccg acccgcccgg
     6001 cgacgtcaac gccactcccg aatacaagcg ccgcctggcg cgagtgctgt gccgcagggc
     6061 gctcgaggag gccgccggaa cagggtgagc gggacagcgc gcgccacagg cggcatcgtg
     6121 ctggccgcgg gagagggccg tcgcttcggc gggcgcaagc agctggccga gctgcacggg
     6181 cgcccgctgc tcgagcacgt cctgagcgcg atggccgcta cgccgctcga gcgagtggtt
     6241 gtcgtgctcg gcgcaaacgc cgacgaggtg ctctcgggcg ttgacctgta cggagcggaa
     6301 ccggtggtct tcgccgcctg gcacgagggg caggccgcct cgctgcgtgc cggcctggac
     6361 gcgctgggcg acgttgcggg cgcggttgtc gcgctcggcg accagccgag gctgtcaccg
     6421 cttgcggtcg agcgactgct gtcggccccg acggacggat tcgacgcggc gcgggcgacg
     6481 tacgccggca agcccggtca cccggtgctt ctgcgccgct ctctgttccc ggccgtccgg
     6541 cagttgtcgg gcgacgaggg tgcgagaggg ctcctcgagc acgcacgagt cgcgtcggtg
     6601 gcctgcgacg gcctcggcgg gcccgaggac gtcgacactc gcgacgagct cgaggagctc
     6661 aaccgatgaa actcgcgcag gccttcgagg tgaccgctcc cgtggagcgc gtgtgggcaa
     6721 cgctcatcga tgtggaggcc gtggctccgt gcctgccggg agccgaggtc accgccagcg
     6781 atgaaaacgg tgtctaccac gggacgttcc aggtcaagat cggggcggcc accgccgcct
     6841 accgcggcac gctctcgatc gacgcggtcg acgaggcgac ccgcaccgtg acgttgcggg
     6901 cgaatgggca ggacaagcgc ggtcaagggt cggccagagc caccatcgtg agcacgctga
     6961 tccccggcga ggccggcgcc acccatgtgg aggtcgagac cgacctgact atcaccggac
     7021 gcctcgccag cttcggccgg ccgggcgtga tgcaggacgt ggctaatcgg ctgctgcgtg
     7081 actttgcccg gtgcctcgag caacggatcg gggcgggcgc ctctgcgggc gccgatccct
     7141 agcgatggga tcccggaagg gagcagcccg cgccggagcg cgggctgccg agtgaggggt
     7201 ctacgccacg tgcctcctca cacgtggctc gtgacccctg cggggcttga tgcgtcggtt
     7261 gcgccggcct ccgcgttctc gtaccacgag agcgcgtagc ggccgtcgtc cagctcgcgc
     7321 gcgagctggg tgaggcggag cggccggaac gtgtcgcaca tcacagccag ctcgttggtg
     7381 tgcttcatcc cgatcgactt ctccgcgagc cccggctgcg gcccatgcgg gagaccggag
     7441 ggatggagcg tgatcgagcc cacgtcgatc cccttgcgcg acccgaactc gccggagacg
     7501 taatagatca tctcctcgct ctgcaggttc gagtggtggt acgggatcgg gatcgcgttc
     7561 tcgtcgaagt cgagcatgcg cgggcagaac gagcagatca cgaagttcgg gccctcgaac
     7621 gtctggtgca ccggcggagg catgtggatg cgcccggtga ccggctcgaa gtcgtcgatg
     7681 ttgaacgtgt acggatagac gtagccgtcc cagcccacga cgtcgagcgg gtgatagtcg
     7741 agcgtgtagt gctggtaacc gtcgcgcacg cgcacgacca gttcgtactc gcccgtgtcg
     7801 cggtgagtga cgagctcggt gggcgaacgg aagtcgcgat gcgagtaggg cgcgtgctcg
     7861 agcagttgcc cgtagcggtt gcggtagcgt ttcggcgtct cgatctcgcc tggcgtgtgg
     7921 aaggtgagat ggagctgctc gccctccggg cggaagcggt acgtggtgcc gcgcgggatc
     7981 acgatgtagt cgtgctcgga atacggcagc gtgccgaaca cggtctcgag ctcgccgcgg
     8041 ccctcgtgga cgaagacgac ctcgtcgccc tcgccgtttc tgtagaagta gtcgtgaccc
     8101 gtctcgggac ggcagatcgc gatctccacg tcgccgttcc acatgagcag ccggcgcccg
     8161 cggatcgggt cgccctccgg ctcgatgccc ttcgtcgcga agtgatggtg ggcgtgcgcg
     8221 tcgggcaccc actcctcgcg cacgatcggc tcgaactcgc cgagccgcat cacgcggcag
     8281 ggtgagacca ggtggtagag gatcgactcg ttgccggaga agccctcgaa ccccatgacc
     8341 tcctcggtca gcagggtgcc gttgtcgcgg aagaccgtgt ggcgcttctt cgggacgtcg
     8401 cccctgagct cgtacctcac aggttccccc tcctctcctg ctcccgctcg atcgcctgga
     8461 acagggcctt gaagttgccc tcgccgaagc cgcgcgcgcc gtggcgctcg atcacctcga
     8521 agaacaccgt ggggcggtcg ccgacgggct tcgtgaagat ctgcagcaga tagccctcgt
     8581 cgtcgcggtc cacgaggatg ccgagccggc ggagatcgtc gagcgactcg tcgatctcgc
     8641 cgatgcggtc cggtacctcg tcgtagtact cgcccgggat cgccaggaac tcgatcccgc
     8701 gcgcggtgag ctccgcgacc gtgttgacga tgtccgtcgt ggcgatcgcg atgtgctgcg
     8761 cacccgcccc gcggtagaag tcgatgtact cctcgatctg cgacttgcgc ttgccggtcg
     8821 ccggctcgtt gatcgggaac ttgatgcgac cgtggccgtc agtgacgacc ttcgacatga
     8881 gcgcggagta ctcggtggag atcgcctcgt ccgagaagtg gatcatctcg gtcatgccga
     8941 agacgcgctc gtagtagtgc acccactcct ccatgtggcc gagctcgacg ttgcccacga
     9001 tgtgatcgat gccgacgaac atgccgtcgc ccgccttcgg gcgcggcgcg cgcgcctcgt
     9061 agccggggag gaacggaccg tcgtactcgc ggcgctgcac aaaggtgtgc ctggtctcgc
     9121 cgtaggtggc aatcgacgcg acctgaacac gaccgtgctc gtcctcgagc cagtgcggtg
     9181 tgctcacccc gcgggcgccg tggtcgaccg cccactcgta cgccgccgcc gcgtccggca
     9241 cgctcagcgc tatgtcgatc acgccgtccc cgtggcgcct gtggtggtcg gcgatttcgt
     9301 cctcgccggt gagagtccca gtcagcacga gccggatgcg accctgctcg accacgcgcg
     9361 agaccctgtc gcgcgtgccg gtctctagcc cacggaaggc agtctccgtg aagccgaagc
     9421 cgtgcacgta gaagtaggcg gcctgcgcgg cgttgcccac gtacagctcc acgtggtcga
     9481 tgccgtggat cggcatccgg tcgtccgcgg gtgcgggcgg cgccgtctgc gggcgcgcgc
     9541 cgtgaagctg ctcgatggcc atcgcgctgt ctcctccctt gttcctccgc ttgcctctat
     9601 gtaatgatcc gagcctcgga cagcggtttc aacgcctgtt tgttgcacga tggctacgtt
     9661 tcagccccaa ttcgacgagg tcgaccgcca aatcgttgcg ctactgcgtg cggatgcccg
     9721 gcgctcgttc caggacatcg gcaagcacgt gcacctcacc gctccggccg tcaaacggcg
     9781 cgtcgatcgc ctcgagcgcg accgcgtgat ccgcggctac acgaccgtcg tggaccagga
     9841 ggcgttcggc tggggcagcg aggcgttcgt gttcctctac tgcgagggac gcatgccggc
     9901 gagcgcgatt cgcgatgcgg tcgagaagga gccgggcgtt gcatccgctc acaccgtggc
     9961 cggcgagtcg agcgcgatcc tgcacgtgct cgcgcgggat acgaaggaat tggagctcac
    10021 cctcgagcga atccgccaca acgagggtgt cacgcgcacg gttagccagg tcgtgctttc
    10081 cacacttttc cagcgatgag ccgccgcgcc taccctgccg gcgcgcagcc gccctcggtg
    10141 cgcgacttct acgccttcga ggagcacgtg cgcaacgctc gccggggacg cggcctcgac
    10201 gtgccgaccg agtggtacga gatccccgtc ttctacttct cgaatccggc ggcgatctac
    10261 ggcgacggcg acgccgtgcc gatgcctgcc gacaccgcag agctcgacta cgagctggag
    10321 ctcgcctgcg tgatcggtcc cgacgggcgg cccgaggggt tcaccgtgat gaacgactgg
    10381 tcggcgcgcg acctccagcg gcgcgagatg cgcgtggggc tcgggccggc caaggggaag
    10441 gacttcgcca cctcgctcgg gcccgagctc gtgagcccgg acgagttgcc ggccgatctc
    10501 gacatgcgag cggtggcacg cgtgaacggc gaggtgcgga cggactcgcg cacgggcggc
    10561 atgcagttct cgtgggacga gctgctcgag gcggcggcgc gcaacacgcg cggcctgcag
    10621 ccgggcgacg tggtcggctc cggcacggtc ggtgccggct gcatcctcga gcacggcgac
    10681 ggccgctggc tcgagcgcgg cgacgtggtg gagctcgaga tcgagggcat cggcgttcta
    10741 cggaacaccg tcgcctgagc ctcaggtctt tggcctgcgc tcctccgcga gcggcttgcc
    10801 ggcctcgtcg gacaggccgg gcggcggcca gggaccgaac cagttgtcgc gcgccccgtc
    10861 gatcgtgatg atcgagccgg agtagtagtc gcccgcgggc gaagccagga acgcgacgag
    10921 ccacgcctgc tcctcgggcc ggcccaggcg ctgaagcggg accgtacgcg cgaccccggc
    10981 gcgcaccggt tcggggtact tctcgatcgc ctcggtctcg aagtggccgc tggcgatcgc
    11041 gttcagccgg atgccgaagc gcgcccactc gatgctcagc acccgcgtca tgttctccac
    11101 cgccgcccga gccgccgagg agtgcgccat gcccggcagc ccgtggtgcg gtgacagtgt
    11161 gacgttcacg atcttgccgc cacgcgactc cggcatcatc gccttcgtcg cgaccgcgtg
    11221 cgtcatcagc caggtgccct cgaggttgac ccggacgacg gtgcggaagc ccttggggct
    11281 gatgtcctcg gcgggcgtca tgtactggcc gcctgcgttg ttgacgagcg tgtcgatgcg
    11341 cccgtggcgc tggagcacgc ggtccacgag cgcctcgacc tgatcctcct cgcggatgtc
    11401 gcaggcgatc gcctcgcagc ggccgccctc gcacatcgcc gccgtctcgt cgagcggctc
    11461 ctgtctgcga cccgcgatca ccacgcgcgc gccgagcgct gtgagctcga gcgcggtggc
    11521 gcggccaagc ccggtaccgc cgccggtcag caggacgatc ttgtcgtcca gcagatccgg
    11581 cctaaatatc tgggagggca tggcggcggg accttatcgc ggccggaaag cgtgtcgcgg
    11641 gcgccgccga tagattgccg cttcgtcggt ggaggcagcc acccaaactc agccgcgcgt
    11701 cgggcagggc gcgctcgaac gcctcttcaa gatctccgag cgaggctcga acgtgcgcat
    11761 cgagctgctc ggcgggctca ccacgttcct cacgatgagc tacatcctct tcgtgaaccc
    11821 cgcgatcctc tcgacgaagg ggaccgggat gcccttcgag gctgtcgcgg tggccacggc
    11881 cctcgcggca tgcatcgcca cgatggccat gggcctgttc acgaactacc ccttcgcgct
    11941 cgcctcgggc cttgggctga acgccgtcgt ggcgttcgac atcatcctcg gccgcaacct
    12001 ccggtgggag gtcggcatgg cctgcatcgt gatcgagggc ctgctcgctc tcgtgctcgt
    12061 gatcgcggcc tgcgcgaagc catcatgagg gccgttccga cgtcgctgaa gctgtcgatc
    12121 ggcgttggca tcggactctt catcacgctc gtcgggctgc gcgacggcgg catcgtggtg
    12181 aacgacccgg cgaccggcat cggcctcggc gacctcacgc acggcgcgcc gctgatcgcg
    12241 ctcggcggca tcctcgtggc cgtgctgctg tcggtgcgca acgtgcgcgg cgccatcctg
    12301 cttggcgtac tcgcgagcac cgcgctcggc ctgatcttcg gcgtgctcga cgggccatcc
    12361 aaaatcgcgg agatccccgg ctcgggcagc ttcagcacga tcggcgacgc gtggaagccc
    12421 gacaacctga gcgatgcgct ccaggtggcg ctgatcccga cgatcttcgc gttgttcatg
    12481 accgacttct tcgacacgat cggaacggtc gtggcggtgg gcggcagcgg cgggctactc
    12541 gacagggccg gcaacctgcc ggggatccgc agagtgctcc tcgtcgactc gggcgcggcg
    12601 gcgctcggcg gcaccttcgg gcctcgtctg tcacgaccta tgtggagagc ggcgcgggcg
    12661 tgagcgaggg cgcgcgcacg ggccttgcct cggt
//
LOCUS       DASVXS010000220        15595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_556488, whole genome shotgun sequence.
ACCESSION   DASVXS010000220 DASVXS010000000
VERSION     DASVXS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15595
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556488"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /gene="mihF"
                     /locus_tag="VF752_12230"
     CDS             <1..358
                     /gene="mihF"
                     /locus_tag="VF752_12230"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX8856355.1"
                     /translation="SPPMSPAAPSTPKHATGVAPERSLTQRMDALRRANEIRTRRASL
                     KRDLKAGRRQIHGLLLDPPEYLLTAKVFDLLLAVPKYGRVKVNRILTQCRISPSKTIG
                     GLSQRQRHELVSFLRR"
     gene            382..966
                     /gene="gmk"
                     /locus_tag="VF752_12235"
     CDS             382..966
                     /gene="gmk"
                     /locus_tag="VF752_12235"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX8856356.1"
                     /translation="MVTGPSGVGKGTLIRSVIERFPGLELSVSATTRKPRPGEVDGRD
                     YHFLSPEEFDRRLADGEFLEHAEYAGNRYGTLRSELDRLSEMSSAGDDQIRGLVLEIE
                     LQGARQVREALPEAVQIFVEPPSLDALRTRLIGRGAESQEQIERRLEVAERELAAKEE
                     FNYRVVNDRLEDAVEELSRLVATIWGQQTSGERE"
     gene            963..1193
                     /gene="rpoZ"
                     /locus_tag="VF752_12240"
     CDS             963..1193
                     /gene="rpoZ"
                     /locus_tag="VF752_12240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX8856357.1"
                     /translation="MIKPRLDDLLEHVDSHYACVLVAAKRARQINSYYHNLGEGTFDE
                     YPPPMVETGSKNYLGIALEEIAAGKLKYRYRT"
     gene            1210..2490
                     /gene="coaBC"
                     /locus_tag="VF752_12245"
     CDS             1210..2490
                     /gene="coaBC"
                     /locus_tag="VF752_12245"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX8856358.1"
                     /translation="MARILLGVSGGIAAYKAVELVRLATKAGHAVRVIQTPAAQRFVG
                     AATFEGITGAPVLVDEFELDPARGAFPGDPAPEHAPISHLELVRRCDVYTIAPATANT
                     IAKLANGLADNLLTSAALACPAPLVIAPAMNNLMFEHPATRENLRVLEARGARIVAPD
                     TGSLASKGEHGIGRLAEPAALLADIESALGAAGYMPHSLDGLRVLVTAGGTREPIDSV
                     RYVGNRSSGRMGFALADEAARRGAEVTVIAANVSLPRSPGVRYVEVVTAAELEQAARA
                     EFDSADVLLMAAAVADFRPTAPEESKIKKDGRDRLEIEMEPTVDVLASLAGGRRPGQT
                     LVGFAAEHSGDYLQLAREKLDRKGLDAVVVNDISCPEIGFDSDENEVAIVTGAGSTEV
                     PRVSKAEVARAVIDVVERLRAETEKTAPREVGSS"
     gene            2487..2882
                     /locus_tag="VF752_12250"
     CDS             2487..2882
                     /locus_tag="VF752_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8856359.1"
                     /translation="MSERDRSPEEERGERVYDLFTRGSALLEAGDYNAASIPLEKARD
                     LEPDKSSIREALGRAYFRSGRYAAAGEEFEAVVERHPMNDFAHFCLGRSYEKTGRRKE
                     ARRHLALAASLQPQRADYRVYRARIDRAA"
     gene            complement(2991..4199)
                     /locus_tag="VF752_12255"
     CDS             complement(2991..4199)
                     /locus_tag="VF752_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX8856360.1"
                     /translation="MLLLFVALAVVIAPPTGARAAPPGLTPPGSMDALGDSFTTGLYS
                     DGCSTDPSCAENSWSTGTTPAVQSHYQRLVPLNPALSGQAVNRAVSGRNMDYLDTEVE
                     AAVADAPAYVTILMGVNDVCGGSLTPATMTEPSVLGTQFRTAMSDLTGNLPGVRVFVA
                     SIPDPYRLWEILHTNTSATAAWSSQEVCPTMLENPTSMAPADVERRAMVRQRVIDDNA
                     QLRAVCAEFAQCLFDNEAVFNWQFAEGDVSTEDYFHPSLQGQAGLAAVTYQAGYDFGA
                     RAPNAQPQVSPADETRPLITAFGRSARLSRRGTISFSATSSENVTGRVTATVSVPSTS
                     KLLRFRSRHVSLTANVRRKITLRLARRNAAKARAVLRRGKRLTVRVTLALSDLAGNAA
                     TRKLSLRLKR"
     gene            4456..5301
                     /locus_tag="VF752_12260"
     CDS             4456..5301
                     /locus_tag="VF752_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX8856361.1"
                     /translation="MSPDLARRLVAEALGTALLVTFGAGSAVAGLRSGGGRLEYAGLG
                     MVAIAFALVIAVAIYAFGKTSGAHINPAVTIALVARGRFPAAEAVPYIVVQLIGAFLG
                     ALLIVAAFGKEAIDLGGVGGTHLADGITFWRGVIAETIATFLLVTAIFALAVDRRAPT
                     GWAGLIIGLSVACGILMIGPITGGSLNPARTFGPLLTTSLWGGDTDWNDFPVYIIGPV
                     AGGVLAAFTYDYVAQMRAAEEPVVEPPQGAAGDITGRQPAGSAVGEPAQGAAGDIAGR
                     QEPMR"
     gene            5327..6586
                     /gene="fdhA"
                     /locus_tag="VF752_12265"
     CDS             5327..6586
                     /gene="fdhA"
                     /locus_tag="VF752_12265"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR02819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HEX8856362.1"
                     /translation="MAEENRGVVYMGPGTVEVQDIDFPSFELKPGPGVPEESIGRKTN
                     HGVILRIVSTNICGSDQHMVRGRTTAPPGLILGHEITGEVIEKGRDVEFLNEGDLVSV
                     PFNIACGRCRNCKERKTGICLTVNPSRPGAAYGYVDMGGWPGGQAQFVMVPYADFNCL
                     KFPDKDQAMEKILDLTMLSDIFPTGYHGCVSAGVTTGSTVYVAGAGPVGLAAAHAAQL
                     LGAAVVMVGDLIPERLEQARSFGCEPIDVSKGDPKDQIANLIGEQLAEGINPPTHIPE
                     IDAGVDAVGFEARGHGADAEEELPATVLNSLMDLTRAGGKLGIPGLYVTGDPGAKDDA
                     AQHGSLSIRIGLGWAKSHEFMTGQTPVMMYNRELMMAILTDRCQIAKAVNATVIPLDD
                     APQGYKDFDQGAARKYVLNPNGLIPQA"
     gene            complement(6640..6828)
                     /locus_tag="VF752_12270"
     CDS             complement(6640..6828)
                     /locus_tag="VF752_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856363.1"
                     /translation="MSAESRLDALRAEYHELIHSMEFAYAMGHSRTLGKDPRLEPYVD
                     RYHELRGQIAALEAEIDV"
     gene            complement(6882..7652)
                     /locus_tag="VF752_12275"
     CDS             complement(6882..7652)
                     /locus_tag="VF752_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX8856364.1"
                     /translation="MREQARFYELSRAGERAALVTVVSGEPVSAVGARMLVLPDAQVD
                     GSLGSDELDSAALAVAEELIWAERSELREAGDVGLFVDVSAPAPRILVFGAVDFAAAL
                     CRVARATGWRPYVIDPRSRFATPERFPDAEQVIAAWPEEALRTLDGIDRATYIAVLTH
                     DPKLDDAALTLALASPAPYVGAMGSRRAQADRRARLLASGVSEEQIDRIAAPIGLDLG
                     ARDAEETALSIMAEVVAVRNGRVGGRLSESKGRIHADP"
     gene            complement(7649..7963)
                     /locus_tag="VF752_12280"
     CDS             complement(7649..7963)
                     /locus_tag="VF752_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX8856365.1"
                     /translation="MTDVLADVQQWSARGDSVAVATVVATRRSAPLPPGSKMAISSRG
                     EVSGAVSGGCVEGAVVEVAEEILSGAEPRLLHFGIADSEAWDVGLPCGGEIDVFVERY
                     KP"
     gene            complement(7960..8967)
                     /locus_tag="VF752_12285"
     CDS             complement(7960..8967)
                     /locus_tag="VF752_12285"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8856366.1"
                     /translation="MTFAQLQAFATVARLGSVKAAARSLGVSEPAVSGAVAALRRDFG
                     DELFERRDGGIALTPGGARLASAASEILGLAARAQRSVTEAQGAAALLRVAVTPAISE
                     QVAPPVFDAFTGRVQNVELSVEVHPSTAFAGLLEDRIADVVLGPRLSGDASFGTESVP
                     FLRYRLVVVARSSHRLARRREIAPAELAGERWLVGPAGAGPETAVGAFFERHRLAPDE
                     VKAFPTYAAAARAAAAGEGVALAITHTVLDDLKRASLARLDIRGTPVERLWYASALGP
                     DRRSAQAWALLRFITTPEATQAMLARSGGVPADRFRSPVYVTLWSSHRLPRARTKEVQ
                     S"
     gene            9089..9955
                     /locus_tag="VF752_12290"
     CDS             9089..9955
                     /locus_tag="VF752_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX8856367.1"
                     /translation="MQVPAPFEYERATSVEDALSLLERLGPEARLIAGGHSLLPMMKL
                     RLAAPEHLIDINDLAALDFIREEGGDIWIGALTRHRELLESELLGKHFPIFHDAERVI
                     ADPLVRNRGTIGGALCQADPSEDLSAVCAAVKAQAVIRGSQGERVVSMDEFHRGPYET
                     AVGDAEMLVEIRLPVREGAGSAYEKLERRSGDWAVAASGVCVWTDGGTITDAGVALAA
                     VGSNAVRAARAEEALRGERPSEELFDEAARLAAEDCSPASDQRGPAEYKRHLAGELTK
                     RALRRAVERALT"
     gene            9967..10458
                     /locus_tag="VF752_12295"
     CDS             9967..10458
                     /locus_tag="VF752_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8856368.1"
                     /translation="MQVNLTVNGEEVTREIEPRLLLVHFLRDTLDLTGTHWGCDTSNC
                     GACTVLMDGEPVKSCTVLAAMAGGHEVRTIESMEKDGALDPVQQGFMEEHGLQCGFCT
                     PGMILTARALLDRNPNPSDEEIREAISGQICRCTGYVNIVKSVRWAAEHSGAATAEPA
                     ATS"
     gene            10467..12827
                     /locus_tag="VF752_12300"
     CDS             10467..12827
                     /locus_tag="VF752_12300"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEX8856369.1"
                     /translation="MATVEERPVGYGRMKRKEDPRFIRGKGNYVDDVKLPGMLHGAVL
                     RSPLAHARIVSIDTSAALQHPKVHAVITGKDLEAQKLAWAPTLSADVQAVLALDKVCF
                     QGQEVAFVVADDRYSARDALELIDVEYEPLPAVVDARKALDPDAPVIREDLEGKTDNH
                     IFDWQAGDKAATDAVFEKADVVLVQDMIYPRVHPAPMETCGCVADFDAVDGKLTLWCT
                     TQAPHAHRTLYALVAGLPEHKIRVISPDIGGGFGNKVGIYPGYVCAVVASIVSGHPVK
                     WMEDRTENLTSTAFARDYVMRGEIAATKEGKIQAVRVNVIADHGAFNAAAQPTKYPAG
                     FFHIFTGSYDLEAAHCAVTGVYTNKAPGGIAYRCSFRVTEAAYVVERMVDVLADELGI
                     DPAELRLKNFIRPEQFPYENKTGWVYDSGEYERAMRKALEMAGYDQLREEQKAARERG
                     ELMGIGVSFFTETVGAGPRKQMDIAGLGMADGATLRVHPTGKAVLGISVQSQGQGHET
                     TFAQIVAEELGIPPEDIEVVHGDTDETPFGLGTYGSRSTPVSGAATAVVARKVREKAR
                     LIAAAMLEASPKDLEWEKGRWFVKGDPEQGKTIQEIALGAHGTVDLPEGVEGGLDAET
                     VYNPVNLTFPFGAYICVVDVDPGTGHVKVRRFVAVDDCGTRINPMIIEGQIHGGLAEG
                     VGIALMELISFDEEGNCLNGSFMDYLIPTSMEVPDFELGETVTPSPHHPIGAKGVGES
                     PTVGSPPAIVNAVIDALEPYGVRHFDMPCSPARVWAAMRGSVEPPA"
     gene            12827..13714
                     /locus_tag="VF752_12305"
     CDS             12827..13714
                     /locus_tag="VF752_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX8856370.1"
                     /translation="MGPELTRRADALSAERVPFVVATVVRAQRPTSVRAGDSAIVLGD
                     GTIEGFVGGACAETSVRLHALRVLETGEPLLLKIVPEGGEAATEEGAVVERNPCLSGG
                     ALELFLEPRVPAPRIAVVGETPIGFALARVGEEVGFDVEVGASGPELGDQVALVVASH
                     GREEDAALAAALTARVPYVGLVASRRRGEAVIESLRQAGVGEEDLGRLHTPAGLAIGA
                     ETPAEIALSILAEIVAERQSPRAEEAATAVDPVCGMTVAISAATPSADLDGRRFWFCG
                     DGCREAFEADPSRYAAGAR"
     gene            13695..14300
                     /locus_tag="VF752_12310"
     CDS             13695..14300
                     /locus_tag="VF752_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8856371.1"
                     /translation="MPQEPASQFTTGLVMAAGGSSRLGRPKQLLPYGEGTLLGHVVDT
                     ARRCSFDQLLVAVGGSADEVTAGADLNGVEVVVNEEYGTGCASSLRTGIQAADPRWTM
                     LVLILGDQPGVTPASVEALLAGRGDAPLAVCRYDDARGHPLAVARELESGLLALHGDK
                     GVWKLMDRLGDQVAEVRVPGPVPVDVDTWDDYEAVLAAAAR"
     gene            14297..15214
                     /locus_tag="VF752_12315"
     CDS             14297..15214
                     /locus_tag="VF752_12315"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8856372.1"
                     /translation="MSSSARSGAGEAVRAAVPDAGALALRLDEVDYLADPGLATVMFL
                     SLRLPQPLLLEGEAGVGKTEAAKALALALDTPLIRLQCYEGIDAAEALYEWNYPRQLL
                     GIRLAESRGRELSENELFGEEYLIRRPLLQAIEHEGPRPAVLLIDELDRADDDFEAFL
                     FELLAEASVTVPEIGTIRAKVPPAVVLTSNRTRDLHDALKRRCLYHWIDYPSPEREVE
                     IVRRRVPESSAELARDAAAALQRLRASDVQKPPGIAEAIDWVAALELLGAERLDEDAA
                     QRTLGSVLKYREDQELVRERGLDWLVGAG"
     gene            15216..>15595
                     /locus_tag="VF752_12320"
     CDS             15216..>15595
                     /locus_tag="VF752_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856373.1"
                     /translation="MAGSVDLPRAAVAFGRRLRAAGLPVTPGRAARFAESLALLAPLT
                     RERVYWAGRASFVSGRGDVDVFDRVFGQVFDGLLDPADARGERGSEPVQAGEPGDRAA
                     PAGAREVAPLGGSGLGLVAGPRGSG"
BASE COUNT         2393 a         5319 c         5692 g         2191 t
ORIGIN      
        1 atcgccgccc atgtcgcccg ccgcgccctc gaccccgaag catgcaaccg gggtcgcacc
       61 agagcgctct ctgacccagc ggatggacgc cctcaggcgt gccaacgaga tccggacccg
      121 ccgcgcgagc ctgaaacgtg acctcaaagc ggggcgcagg cagatccacg gcctgctgct
      181 cgatccgccc gagtacctcc tcaccgccaa ggtgttcgac ctgctcctgg cggtgccgaa
      241 gtatggccgc gtgaaggtga atcgaatcct cacgcagtgc cggatctccc cgagcaagac
      301 gatcgggggc ctgtcccagc gccagcggca cgagctcgtc tcgttcctgc gcagataagc
      361 gccctgaagc cgcaagttct cgtggtgacc ggcccgtccg gggtcggcaa ggggacgctg
      421 atccgctcag tgatcgagcg cttcccgggt ctcgagctgt cggtgtcggc caccacccgc
      481 aagccccggc cgggcgaggt cgacgggcgc gactaccact tcctctcgcc cgaggaattc
      541 gaccggcgcc tggccgacgg ggagttcctc gagcacgcgg agtacgccgg aaaccgctac
      601 ggcacgctgc gctcggagct cgacaggctc tcggagatgt cctcagcggg tgacgatcag
      661 atccgggggc tcgtgctcga gatcgagcta cagggcgccc gccaggtgcg cgaggccctg
      721 cctgaggcgg tgcagatctt cgtggagccg ccgtcgctcg acgcactgcg cacgcgcctc
      781 atcggacgcg gggcggaatc ccaggagcag atcgagcggc gcctcgaggt cgcagagcgc
      841 gagctcgccg cgaaggagga gttcaactac cgcgtggtga acgaccggct ggaggacgcg
      901 gttgaggagc tctcgcgcct cgtcgctacc atctggggcc agcaaacgtc aggagaacgc
      961 gagtgatcaa gccacgtctc gacgatcttc tcgagcacgt cgactcccat tacgcgtgcg
     1021 ttctggttgc agccaagcgc gctcgtcaga tcaacagcta ctaccacaac ctcggcgagg
     1081 gaaccttcga cgagtacccg ccgccgatgg tcgagacggg ctccaagaac tacctcggca
     1141 tcgccctcga ggagatcgcc gcgggcaagc tgaagtaccg ctaccggacc tagtccgagg
     1201 agggccggcg tggccaggat cctgctgggc gtctccggag gaatcgccgc ctacaaggct
     1261 gtggagcttg tgcgcctcgc caccaaggcg ggccacgcgg tgcgcgtgat ccagacgccc
     1321 gccgcgcagc gcttcgtggg cgccgccacg ttcgagggca tcaccggcgc gccggtcctg
     1381 gtcgacgaat tcgagctgga ccccgcgcgc ggcgccttcc caggcgatcc ggcgccggag
     1441 cacgcgccga tctcgcacct cgagctcgtg cgccgctgcg acgtctacac gatcgcgccg
     1501 gcgaccgcga acacgatcgc caagctcgcc aacgggctcg ccgacaattt gctcacgagc
     1561 gccgcgctgg cgtgccccgc gccgctcgtg atcgcgccgg cgatgaacaa tctgatgttc
     1621 gagcaccccg ccactcgcga aaacctgcgc gtgctcgagg cgcgcggggc gcgaatcgtg
     1681 gcgcccgaca ccggctcgct cgcgtcgaag ggcgagcacg gcatcggcag gctggcggag
     1741 ccggcggcgc tgcttgccga catcgagagt gcgctcggcg ccgcgggcta catgccgcac
     1801 tcgctggacg gcctgcgcgt gctggtgacg gccggcggca cccgggagcc gatcgactcc
     1861 gtgcgctacg tgggcaatcg ctcttccggc cgcatgggct tcgccctcgc cgacgaggcg
     1921 gcgcggcgcg gcgcagaggt aaccgtgatc gccgccaatg tgtcgctccc gcgttcgccc
     1981 ggcgttcgct acgtggaggt ggtcaccgcc gccgagctcg agcaggcggc gcgcgccgag
     2041 ttcgactcgg ccgacgtgct gctgatggcc gcggcggtcg ccgacttccg gccgacggcg
     2101 ccggaggagt cgaagatcaa gaaggacggc cgggaccgcc tcgagatcga gatggagccg
     2161 accgtggacg tgctcgcgtc gctggcgggg ggtcgccgcc cgggtcagac gctcgtgggc
     2221 ttcgcggcgg agcactccgg cgactacctc cagctcgcgc gcgagaagct cgaccgcaag
     2281 ggcctcgacg cggtcgtggt gaatgacatc tcgtgccccg agatcggctt cgactccgac
     2341 gagaacgagg ttgccatagt cacaggcgcg ggttcgaccg aagtcccgcg ggtaagcaag
     2401 gccgaggtgg cgcgcgcagt gatcgacgtt gtggagcggc tccgggccga gacggagaag
     2461 acggcgccac gggaggtggg aagttcatga gcgagcgcga tcgcagccca gaagaagagc
     2521 gcggcgagcg ggtgtacgac ctgttcacgc gcggcagcgc gctgctcgag gccggcgact
     2581 acaacgccgc gtcgatccca ctcgagaagg cccgcgacct cgagcccgac aagagctcga
     2641 tccgcgaggc gctcggccgc gcgtacttcc ggagcggtcg ctacgccgcc gccggcgagg
     2701 agttcgaggc ggtcgtcgag cgccacccga tgaacgactt cgcgcacttc tgcctggggc
     2761 gctcatacga gaagacgggc cggcgcaagg aagcgcgccg ccacctcgca ctcgctgcct
     2821 cgctgcaacc acagcgggcg gactaccgcg tgtaccgcgc ccgaatagac cgcgcggcct
     2881 gatcccggtg caaaccttcg ccatgctgcg tcctcgggcg ctcgccaagc ttcggccctg
     2941 cgtcctcgcc tgactcggtt ttcgccggga tcacgcctgc ggtgccgcgg ctaccgcttc
     3001 agcctcaggc tcagcttcct cgtggccgcg ttgccggcga ggtcgctcag cgccagggtg
     3061 actctgacgg tgaggcgctt gcctctcctc agaacggccc gtgccttggc ggcgttccgc
     3121 cgcgcgagcc tcagcgtgat tttcctccgc acgttcgcgg tcagagagac gtggcggctc
     3181 ctgaagcgca gcagctttga agtgctcggc acgctgaccg tggcggtgac cctgcccgtg
     3241 acgttctcgc tcgacgtggc ggagaaagag atggtcccgc ggcgcgagag gcgcgccgac
     3301 cgtccgaacg ctgtgatcag cggccgggtc tcgtcggctg gcgaaacctg cggctgtgcg
     3361 ttcggcgcgc gggcgccgaa gtcgtagccg gcctggtagg tgacggcggc gaggccggcc
     3421 tggccctgga gcgaggggtg gaagtagtcc tccgtgctca cgtcgccttc ggcgaactgc
     3481 cagttgaaca cggcctcgtt gtcgaagagg cactgcgcga actcggcgca gaccgcgcgc
     3541 agctgcgcgt tgtcgtcgat cacgcgctgg cgcaccatcg cgcgccgctc gacgtcggca
     3601 ggcgccatgg aggtcgggtt ctcgagcatg gtcgggcaca cctcctggct gctccaggca
     3661 gcggtggcgc tcgtgttcgt gtggaggatc tcccagagcc gataggggtc cgggatgctc
     3721 gcgacgaaca cgcgcactcc cggcaggttg cccgtgagat cgctcatcgc cgtccggaac
     3781 tgggtgccca ggaccgacgg ctcggtcatc gtggcggggg tcaggctgcc gccgcagacg
     3841 tcgttgacgc ccatcaggat cgtcacgtac gcaggcgcgt cggcgaccgc ggcctccacc
     3901 tcggtatcga ggtagtccat gtttcggccc gacaccgcgc gattgaccgc ctggcccgag
     3961 agcgcggggt tcagcggcac cagccgctgg tagtggctct gcaccgcggg cgtcgtgccg
     4021 gtcgaccagg agttctccgc acagcttggg tccgtgctgc agccgtcgct gtagaggccg
     4081 gtcgtgaacg aatcgccgag cgcgtccatc gagccgggcg gcgtcagccc gggcggcgcc
     4141 gcgcgcgcgc ctgttggagg cgcgataacg accgccaagg cgacgaacaa caggagcaag
     4201 agcgagcccc gatcaggccg tccgagacgc atttttggca cgcaccaacc tctcggtcac
     4261 gcccatcaca acgtggtcaa agcgggcgcg tctgcgcctt gactctctct ataccgggct
     4321 ctatacccgg gtaccggaac agcacccgac agcgggaacc gggatgcaac cgaagcgaga
     4381 ccggcgcctt cgccgcggcc cccgtcggcg cagggagtcg atcggcgctg tcgcccggca
     4441 gaggggggag cggcgatgag cccagacctt gcccgtcgac tcgtggccga ggcgctaggg
     4501 acggcgctgc tcgtgacgtt cggcgcgggc tcggcggtcg cggggctgcg ctctggcggc
     4561 ggccgcctcg aatacgccgg actcgggatg gtggcgatcg cgttcgcgct cgtgatcgcc
     4621 gtggcgatct acgcgttcgg caagacgtcc ggcgcccaca tcaaccctgc cgtgacgatc
     4681 gccctcgtgg cccgcgggcg cttcccggcg gccgaggccg ttccatacat agtcgtacag
     4741 ctgatcggcg cgttccttgg cgcgctgctg atagtggcgg cgttcgggaa ggaagcgatc
     4801 gacctgggcg gcgtgggcgg cactcacctc gccgacggga tcacgttctg gcgaggcgtg
     4861 atcgcggaga caatcgcgac gttcctgctc gtcacggcga tcttcgcgct cgcagtggat
     4921 cgccgagcgc cgaccggctg ggcgggtctg atcatcgggc tgtcggtggc atgcgggatc
     4981 ctgatgattg gcccgatcac gggtgggtcc ctgaacccgg cgcggacgtt cgggccgttg
     5041 ctgaccacct cgctctgggg cggggacacc gactggaacg actttccggt ctacatcatc
     5101 gggcccgtgg ccgggggagt gctcgccgcg tttacctacg actacgtggc ccaaatgcga
     5161 gcggccgagg agccggtggt cgagccgccg cagggcgccg ctggcgacat cacgggccgc
     5221 cagcccgccg gatccgccgt gggggagcct gcgcagggcg ctgcgggaga catcgcaggg
     5281 cgtcaggagc ccatgcggtg aagtgccata cctgaggagg aaagaaatgg ctgaagaaaa
     5341 cagaggcgtc gtatacatgg gcccgggcac cgtcgaggtg caggacatcg acttcccgtc
     5401 cttcgagctc aagcccgggc cgggcgtccc cgaagagagc atcggcagga agacgaatca
     5461 cggggtgatc ctgcgcatcg tgtcgacgaa catctgcggc agcgaccagc acatggtgcg
     5521 cggccgcacg accgccccgc cggggctgat cctcggtcac gagatcaccg gcgaggtcat
     5581 cgagaagggc cgcgacgtgg agttcctcaa cgagggcgat ctcgtctcgg ttcccttcaa
     5641 catcgcctgc gggcgctgcc ggaactgcaa ggagcgcaag accggcatct gcctgacggt
     5701 caacccgtcg cgtcccgggg cggcctacgg ctacgtggac atgggcggct ggccgggagg
     5761 ccaggcgcag ttcgtgatgg tgccgtacgc ggacttcaac tgcctgaagt tccccgacaa
     5821 ggatcaggcg atggagaaga tcctcgacct gaccatgctg tcggacatct tcccgaccgg
     5881 ttaccacggc tgcgtgagcg cgggcgtgac cacggggagc acggtgtacg tcgctggggc
     5941 ggggccggtg ggcctggcgg cggcgcacgc ggcccagctg ctcggcgcgg cggtcgtgat
     6001 ggtgggcgac ctgatcccgg agcggctcga gcaggctcga agcttcggct gcgagccgat
     6061 cgacgtgtcg aagggcgacc cgaaggacca gatcgcgaac ctgatcggcg agcaactggc
     6121 cgaggggatc aacccgccga cacacatccc ggagatcgac gcgggcgtcg acgcggtcgg
     6181 gttcgaggca cgcgggcacg gagcggacgc cgaagaggag ctgcccgcga cggtgctgaa
     6241 ctcgctgatg gacctcacac gcgcgggcgg caagctcggc atcccgggtc tgtacgtgac
     6301 cggcgatccg ggtgcgaagg acgacgccgc gcagcacggc tcgttgtcga tccggatcgg
     6361 cctcggctgg gcgaagtcgc acgaattcat gaccggtcag acgccggtga tgatgtacaa
     6421 ccgcgagctg atgatggcga tcctcaccga tcgctgccag atcgcgaagg cggtgaacgc
     6481 gacggtcata ccgctcgacg atgcgccgca gggttacaag gacttcgacc agggcgcggc
     6541 ccggaagtac gtcctgaacc cgaacgggct cattccgcag gcctgattca acgtatagcg
     6601 gggaggcggc ggtgctttgg cgccgccgcc gctctgcctc tagacgtcga tctcggcctc
     6661 gagcgcggcg atctggcccc gcagctcgtg gtagcggtcg acgtacggct ccaggcgcgg
     6721 atccttgccg agcgtcctcg aatggcccat cgcataggcg aactccatcg agtggatcag
     6781 ctcgtggtac tcggcgcgca gcgcgtcaag gcgggattcg gctgacacga gcgttgatgg
     6841 tagaaggcgc cgcggcggca aaccctgtcg cgtgggcgcg ctcagggatc ggcgtggatc
     6901 cggcccttcg actccgacag ccggccgccc acgcgcccgt tccgcaccgc gactacctcg
     6961 gccatgatcg acagcgcggt ctcctcggcg tcgcgcgcgc cgaggtcgag cccgatcggc
     7021 gccgcgatgc ggtcgatctg ctcctcggac acgccggacg cgagcagccg cgcgcgccgg
     7081 tcggcctggg cgcggcgact ccccatcgca ccgacgtacg gagcggggga ggcgagcgcg
     7141 agcgtgagcg cggcgtcgtc gagcttcggg tcgtgagtga gcacggcgat gtaggtggcg
     7201 cggtcgatac cgtcgagcgt ccgcagcgcc tcctccggcc aggcggcgat cacctgctcg
     7261 gcgtcgggga agcgctcggg cgttgcgaaa cgggagcgcg gatcgatcac gtacgggcgc
     7321 cagccggtcg cgcgagccac cctgcacagc gccgcggcga agtcaacagc gccgaatacg
     7381 agaatccgtg gcgccggcgc tgacacgtcc acgaacaacc caacgtcccc ggcctcgcgt
     7441 agctccgagc gctccgccca gatcagctcc tcggcgacag ccagcgcggc cgagtcgagc
     7501 tcgtcactgc ccaatgaacc gtcgacctgc gcgtcaggga gcaccagcat ccgcgcgccg
     7561 acggcggaga caggctcgcc gctcacgacc gtcacgagcg cagcccgctc gcccgcccgc
     7621 gagagctcgt agaagcgggc ttgctctctc atggcttgta gcgctcgacg aatacgtcga
     7681 tctcgccgcc gcagggcaga ccgacgtccc acgcctcgga gtccgcgatg ccgaagtgca
     7741 gcagccgtgg ctcggcgccc gaaaggatct cttcggccac ctcgacgacc gcaccctcca
     7801 cgcagccgcc cgacacggcg ccggacacct cgcccctcga gctgatcgcc atcttcgagc
     7861 ccggggggag cggcgccgag cggcgcgtcg ccacgacggt ggccaccgcg acggagtcac
     7921 cgcgcgcgga ccactgctgc acgtcggcca gaacgtcggt catgactgga cctccttggt
     7981 gcgagcccgg ggcaggcggt ggctgctcca gagcgtcacg tagacgggtg agcggaagcg
     8041 gtcggcgggc acgccgccgc tgcgggcgag catcgcctgg gtggcctccg gggtggtgat
     8101 gaagcgaagc agcgcccatg cctgcgccga gcgccggtcg gggccgagcg cgctcgcata
     8161 ccagagccgc tcgacgggag tgccgcggat gtcgaggcgc gcgagcgaag cgcgcttcag
     8221 gtcgtcgagc acggtgtgcg tgatagccag cgccacgcct tcgcccgccg cggcggcccg
     8281 cgccgccgcc gcgtaggttg ggaatgcctt cacctcgtcg ggcgcgagcc ggtgccgttc
     8341 gaagaacgcg cccacagcgg tctcggggcc ggcgccggca gggcccacga gccagcgctc
     8401 accggcgagc tcggccggcg cgatctcgcg gcggcgggcg agccggtgcg agctgcgggc
     8461 cacgaccacg agccggtagc gcaggaacgg caccgactcg gtcccgaacg acgcgtcccc
     8521 ggacagccgc gggccgagca ccacgtcggc gatgcggtct tcgagcagtc ccgcgaaggc
     8581 cgtgctcggg tggacttcca cgctcagttc cacgttctgc acgcgcccgg tgaaggcgtc
     8641 gaacaccggc ggcgcgacct gctccgagat ggcgggcgtg accgccacac gcagcagcgc
     8701 ggcggcgccc tgcgcctcgg tcaccgagcg ctgcgcgcgc gcggcgaggc ccaggatctc
     8761 cgaggcagcg ctcgcgaggc gcgcgcctcc gggcgtcagc gcgatccctc cgtcgcggcg
     8821 ctcgaacagc tcgtccccga agtcgcgccg cagtgcggcg actgccccgg acaccgccgg
     8881 ctcgcttacg ccgagcgagc gcgcggcggc cttcaccgaa ccgagccggg cgaccgtcgc
     8941 aaacgcttga agctgcgcga acgtcatccg cggagtctcc tcagtgagcg ccgaattaag
     9001 cgatcgctta agagggcctt gacccttcga tcttacagtg ggacttttcc cgcgccaccg
     9061 ggttgcccgg ctactgaagg aggcccctgt gcaggtaccg gctccgttcg aatacgagcg
     9121 agcgacgagc gtcgaggacg ctctctcgct gctcgagcgg ctcggtcccg aggcgcggct
     9181 catcgcgggc ggtcacagcc tgctgccgat gatgaagctg cggctcgccg cgcccgagca
     9241 cctgatcgac atcaacgatc tcgcggctct cgacttcata cgcgaggagg gcggcgacat
     9301 ttggatcggc gcgctcaccc gccaccgcga gctgctcgag tccgaactgc tcggcaagca
     9361 cttcccgatc ttccacgacg ccgagcgcgt gatcgccgat ccgctcgtgc gcaaccgcgg
     9421 cacgatcggc ggtgcgctat gccaggccga tccatcagag gatctctccg ccgtctgcgc
     9481 ggcggtgaag gcacaggccg tgatccgcgg cagccagggc gagcgcgtgg tgtcgatgga
     9541 cgagttccac cgcgggccgt acgagaccgc cgtcggcgac gccgagatgc tcgtcgagat
     9601 ccgcctcccc gtgcgcgagg gtgcgggcag cgcgtacgag aagctcgagc gccgttcagg
     9661 cgactgggcg gtcgccgcct ccggcgtgtg cgtctggacg gacggcggga cgatcaccga
     9721 cgccggggtc gcgctcgcgg ccgtcggttc gaacgcggtc cgcgccgccc gcgccgagga
     9781 ggcgctgcgc ggcgagcggc cgtccgagga gctgttcgac gaggccgcgc ggctcgcggc
     9841 ggaggactgc tcgcccgcga gcgaccagag gggcccggcc gagtacaaac gccacctggc
     9901 cggcgagctg acgaagcgcg cgcttcgacg cgcggtggaa cgcgcactca cataggagga
     9961 agaaccgtgc aggtgaacct gaccgtcaac ggcgaggagg tcacgcgcga gatcgagccg
    10021 cgcctcctcc tcgtgcactt cctgagggac acgctcgacc tgacgggcac gcactggggc
    10081 tgcgacacga gcaactgcgg cgcgtgcacc gtgctgatgg acggcgagcc cgtgaagtcg
    10141 tgcaccgtgc tagccgcgat ggcgggcggc cacgaagtcc gcacgatcga gtcgatggag
    10201 aaggacgggg cgctcgaccc cgtccagcag ggcttcatgg aggagcatgg gctccagtgc
    10261 gggttctgca ctccgggcat gatcctgacc gcgcgggcgc tcctcgatcg caacccgaac
    10321 ccgagcgacg aggagatccg cgaggcgatc tccgggcaga tctgccgctg caccggctac
    10381 gtgaacatcg tcaagtccgt ccgctgggcg gccgagcact ccggcgccgc cacggcggag
    10441 ccggcggcga cgagttagga ggggccatgg ccaccgttga agaacgcccc gtcggctacg
    10501 gccgcatgaa gcggaaggaa gacccgcgct tcatccgcgg caagggcaac tacgtggacg
    10561 acgtcaagtt gccgggcatg ctgcacggag ccgtgctccg cagcccgctc gcgcacgcac
    10621 ggatcgtctc gatcgacacg tcggcggccc tgcagcaccc caaggtgcac gcggtcatca
    10681 ctggcaagga cctcgaggcg cagaagctcg cctgggcgcc gacgctctcg gccgacgtgc
    10741 aggccgtgct ggcgctcgac aaggtctgct tccagggcca ggaggtggcg ttcgtcgtcg
    10801 ccgacgaccg ctactcggca cgcgacgcac ttgagctgat cgacgtcgag tacgagccgc
    10861 tccccgctgt ggtggacgcg cgcaaggcgc tcgacccgga tgctccggtg atccgcgagg
    10921 acctcgaggg aaagaccgac aaccacatct tcgactggca ggccggagac aaggcggcga
    10981 cagatgccgt gttcgagaag gccgacgtcg tgctggtcca ggacatgatc tacccccgcg
    11041 tccatccggc gccgatggag acctgcggct gcgtggccga cttcgacgcg gtcgacggga
    11101 agctcacgct ctggtgcacc acgcaggctc cgcacgcgca ccgcacgctg tacgcactgg
    11161 tggcggggct gcccgagcac aagatccgcg tgatctcgcc cgacatcgga ggcggcttcg
    11221 gcaacaaggt ggggatctac cccggctacg tgtgcgcggt ggtcgcatcg atcgtgagcg
    11281 gtcatccggt gaagtggatg gaggaccgca cggagaacct cacgagcacc gcgttcgcac
    11341 gtgactacgt gatgcggggc gagatcgccg ccacgaagga ggggaagatc caggcggtgc
    11401 gtgtgaacgt gatcgccgac cacggcgcgt tcaacgcggc ggcgcagccg acgaagtacc
    11461 cggcgggctt cttccacatc ttcaccggct cgtacgacct ggaggcggcg cactgcgcgg
    11521 tgaccggcgt ctacacgaac aaggcacctg gcggaatcgc ctaccgctgc tcgttccgcg
    11581 tgacagaggc cgcctacgtg gtcgagcgca tggtcgatgt cctggccgac gagctcggca
    11641 tcgacccggc ggagcttcgg ctgaagaact tcatccggcc ggagcagttt ccgtacgaga
    11701 acaaaaccgg ctgggtgtac gactcgggcg agtacgagcg cgcgatgcgc aaagcgctcg
    11761 agatggcggg ctacgaccag ctccgcgagg agcagaaggc agcccgcgag cgcggggagt
    11821 tgatgggcat cggcgtgtcg ttcttcactg agaccgtcgg cgccgggccg cgcaagcaga
    11881 tggacatcgc gggcctcggc atggcggacg gcgccacgct gcgggtgcat ccgaccggca
    11941 aggccgtact agggatcagc gtccagagcc agggacaggg ccacgagacc acgttcgcgc
    12001 aaatcgtggc cgaggagctc gggatcccgc cggaggacat cgaggtcgta cacggcgaca
    12061 cggacgagac tcccttcggc ctgggcacct acggcagccg ctcgactccg gtctctggcg
    12121 cggccaccgc ggtggtcgcg cgcaaggtgc gcgagaaggc gcgtctgatc gcggctgcca
    12181 tgctggaggc ctcaccgaag gacctcgaat gggagaaggg ccgctggttc gtgaagggcg
    12241 atcccgagca gggcaagacg atccaggaga tcgcgctcgg cgcgcacggc accgtggacc
    12301 tgccagaggg ggtggagggc ggcctcgacg ccgagaccgt ctacaacccg gtgaacctca
    12361 cgttcccgtt cggtgcctac atctgcgtgg tcgacgtcga cccgggcacc ggccatgtca
    12421 aggtgaggcg cttcgtggcg gtggatgact gcggcacgcg gatcaacccg atgatcatcg
    12481 agggccagat ccacggcggc ctggcggagg gggtcggcat cgccctgatg gagctgatca
    12541 gcttcgacga ggagggcaac tgcctcaacg gctcgttcat ggactacctc atcccgacgt
    12601 cgatggaggt gccggacttc gagctcggtg agaccgtcac gccgtcgccg catcatccga
    12661 tcggggcgaa gggtgtgggc gagtcgccca cggtgggctc gccacccgcc atagtcaatg
    12721 ccgtgatcga cgcgcttgag ccatacggcg tccgccattt cgacatgccg tgctcgcccg
    12781 cgcgcgtgtg ggcggcgatg cgggggagcg tcgagccgcc tgcatgatgg gacccgagct
    12841 gacccggcga gcggacgcgc tctcagcgga gcgcgttccg ttcgtcgtgg ccacggtcgt
    12901 gcgcgcgcaa cggccaacga gcgtccgcgc cggcgactcg gcgatcgtgc tcggcgacgg
    12961 cacgatcgag ggcttcgtgg gcggcgcctg cgcggagacg tccgtgcggc tgcacgcgct
    13021 gcgcgtgctc gagaccgggg agccgctgct cctgaagatc gtccccgagg gcggcgaggc
    13081 agcgacggag gagggggcgg tggtggagcg caatccctgc ctcagcggcg gcgccctcga
    13141 gctgttcctc gagccgcggg tacccgcgcc gcggatagcg gtggtcggcg agacaccgat
    13201 cggcttcgcg ctcgcgcgcg tgggcgagga agtcggcttc gacgtggagg tgggggcgag
    13261 cggtcccgag ctcggcgacc aggtagccct cgtggtcgct tcgcacgggc gggaggagga
    13321 cgccgcactc gcggcggcac tcaccgcccg ggtgccgtac gtgggcctcg tggcgagtcg
    13381 gcggcggggc gaggcggtga tcgagtcgct gcgccaggcg ggcgtcgggg aggaggacct
    13441 tgggcgcctg cacacgcccg cgggcctcgc gatcggggcg gagacgccgg cggagatcgc
    13501 gctctcgatc ctcgcggaga tcgtggccga gcggcagtcg ccccgcgcgg aggaggccgc
    13561 gacagccgtc gacccggttt gcggcatgac ggtcgcgatc tcggcagcca caccctcggc
    13621 cgatctcgac gggcgccgct tctggttctg cggcgatggc tgccgcgagg cgttcgaggc
    13681 agatccgagc cgctatgccg caggagcccg ctagccagtt cacgacagga ctcgtgatgg
    13741 ccgccggcgg gtcgagccgc ctcggccgcc cgaagcagct cctgccgtac ggcgagggaa
    13801 cgctgctcgg gcacgtggtg gacaccgccc gtcgctgctc gttcgaccag ctgctcgtcg
    13861 cggtcggggg gtccgcagac gaggtgaccg ccggggccga tctcaacggc gtcgaggtcg
    13921 tcgtcaacga ggagtacgga actggctgcg cgtcctcgct ccgcaccgga atccaggcgg
    13981 cggacccgcg ctggacgatg ctcgtgctga tcctcgggga ccagcccggg gtgacgccgg
    14041 ccagcgtcga agccttgttg gccgggcgcg gtgacgcgcc gctcgcggtg tgccggtacg
    14101 acgatgcgcg aggccacccg ctggccgtcg cacgcgagct cgagagcggc ctgctcgcgc
    14161 tgcacggcga caagggcgtg tggaagctga tggatcgcct cggggaccag gtcgccgagg
    14221 tgcgggttcc cggcccggtg ccggtcgacg tggatacgtg ggacgactac gaggcggtgc
    14281 tggccgccgc cgcgcggtga gcagctcggc gaggtccggc gcgggcgagg ccgtgcgcgc
    14341 ggcggtgccg gacgccggcg cgctcgcgct gcgtctcgac gaggtcgact acctcgccga
    14401 tccggggctc gcgaccgtca tgtttctcag cctgcggctg ccgcagccgc tcctgctcga
    14461 gggcgaggcg ggcgtcggca agaccgaggc cgccaaggcg ctcgcgctgg cgctcgacac
    14521 gccgctgatc aggctgcagt gctacgaggg catcgacgcc gctgaggcgc tgtacgagtg
    14581 gaactatccg cggcagctgc tcggcatccg gctggccgag tcgcgcgggc gcgagctctc
    14641 cgagaacgag ctgttcggcg aggagtattt gatcaggcgg ccgctgctgc aggcgatcga
    14701 gcacgagggg ccgcgcccgg cggtgctgct gatcgacgag ctcgaccgcg ccgacgacga
    14761 cttcgaggcg ttcctgttcg aactcctcgc ggaggcgagc gtgacggtgc ccgagatcgg
    14821 cacgatcagg gccaaggtgc cgccggcggt cgtactcacg tcgaaccgca cgcgcgacct
    14881 gcacgacgcg ctgaagcgcc gctgcctcta ccactggatt gactacccgt cgcccgagcg
    14941 ggaggttgag atcgtgcgcc gccgcgtgcc cgagagctcg gccgagctgg cgcgcgacgc
    15001 ggccgccgcg ctgcagcggc tgcgcgcgag cgacgtgcag aagccgccgg ggatcgcgga
    15061 ggcgatcgac tgggtggcgg ccctcgagct gctcggcgcc gagcgcctcg acgaggacgc
    15121 cgcgcagcgc acgctcgggt ccgtgctcaa gtaccgcgag gaccaggagc tcgtgcgcga
    15181 gcgcgggctc gactggctcg tgggagcggg ctgaggtggc gggctccgtg gacctgccgc
    15241 gcgctgccgt ggcgttcggg cggcggctcc gtgcggccgg tctgccggtc acgccgggac
    15301 gcgccgcccg cttcgccgaa tcgctcgcgc tgctcgcgcc gctaacgcgc gagcgcgtgt
    15361 actgggccgg gcgcgcctcg ttcgtctccg ggcgcggcga cgtggacgtg ttcgaccgcg
    15421 tgttcggcca ggtgttcgac ggactgctcg atccggcgga tgctcgcggt gagcgcggct
    15481 ccgagccggt gcaggcgggc gagccgggtg atcgcgcggc gcccgccggt gcgcgcgagg
    15541 tcgctccgct cggcggctcg ggcctggggc tagtggccgg cccgcgcggg agcgg
//
LOCUS       DASVXS010000221         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_596241, whole genome shotgun sequence.
ACCESSION   DASVXS010000221 DASVXS010000000
VERSION     DASVXS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596241"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VF752_12325"
     CDS             <1..907
                     /locus_tag="VF752_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8856374.1"
                     /translation="PANESSDAEDRVIELAVDHAFNAGSIVVAAGGNEGSGQVDVPSA
                     YPHVLSVAATDASDLRASFSNFGDFVDLAAPGDSVITTAPRSLCASGYQVASGTSFSA
                     PAVSGAVALVDHVRPGLTVSQRFELVRRSARDVGTPGFDVGTGLGVLDVSNALSGPVP
                     VGDGYEVDDDVYWITGRFAKSHRALLRRTRRSGRATGRVSGFNDPTDVYRVELRKGDS
                     LKATLTGAAGSRFALGIWSPRTRGLDITENRTRGRLLAYRDKPGAGERLTARKVATSG
                     SYYVSVEAPHPDRADTTYVLTVRRG"
     gene            965..2047
                     /locus_tag="VF752_12330"
     CDS             965..2047
                     /locus_tag="VF752_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856375.1"
                     /translation="MRYRAAYIAGALAILLAIPPAGAGAASPAPAGPHVRSGHVQEDP
                     ATRAKRAERRRKIRRQLAREVRRNPRVVMTKRFIRKAGHVRFDLPLTVRLNPLIDDDV
                     NTATPPVFAPSDDTFDYTTDATPVADPAGSLAPLASPLLTGSFKLAARFGKTIVGYDT
                     GSIGVEVGTRDIDLQAGSIPLVQPASPCPDSSPALRTGPISISEISGWSRTGFLALAG
                     GRTSLSLYTQWSFSSLRQGDFTTTPGSFTDGCTGQYFWTKKIGPTLPPVPLEMIGTSA
                     VNPAITTDGKLRFLKLTIDDVVLPQEDFAATIHVCSDADPSETPEASPPPGGICTGVS
                     GDDVPIAARLKVKKLTAEVLLGDLPS"
     gene            complement(2057..2911)
                     /locus_tag="VF752_12335"
     CDS             complement(2057..2911)
                     /locus_tag="VF752_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8856376.1"
                     /translation="MREALARLRGAGSTEQLLSKATVELCRHCGFDRSIFFSVQGSQI
                     APESVHMEAHPSRAAEFLDLVRHSEPQLMPELHETDMIRQRKAVLVEDARNDERTHEA
                     IVRACRTRSYVAAPVAANGRVVGFFAADHFFARTQATPLDREVIWAFAEGFTHAYECM
                     LMQNRLRAQSRRLHEMLAAMESAIAESEGEQLALPAERTSVEGIAPRRLRGSDFADPT
                     EGLLTRREREVLALMAEGATNAMIAERLVISPGTVKSHVKHILRKLRAANRAEAVSRY
                     LRLSSLRA"
BASE COUNT          500 a         1151 c         1143 g          492 t
ORIGIN      
        1 tccggcgaac gagagctcgg acgcggagga ccgcgtgatc gagctggcgg tcgaccacgc
       61 gttcaacgcc gggtcgatcg tcgtggccgc cggtggcaac gagggctcgg gacaggtcga
      121 cgtgccgtcg gcctacccgc acgtgctgtc ggttgccgcc accgacgcga gtgacctgcg
      181 cgcgagcttc tcgaacttcg gcgacttcgt agacctcgcc gcgccgggcg actcggtgat
      241 cacgacagcg ccgcgctcac tctgcgcgag cgggtaccag gtcgcgtcgg gcacgtcgtt
      301 ctcggcgccg gcggtctcgg gtgcggtcgc gctggtggat cacgtgcggc cgggcctgac
      361 ggtctcgcag aggttcgagc tcgtgcgacg cagtgcgcgc gacgtcggca cgcccgggtt
      421 cgacgtaggc accggcctcg gtgtgctcga cgtgtcgaac gcgctctcgg gaccggtgcc
      481 cgtcggcgac ggctacgagg tcgacgacga cgtgtactgg atcaccggcc gcttcgcgaa
      541 gagccatcgg gctctgctgc gccgaacacg gcggagcggc cgcgcgacgg ggcgtgtctc
      601 cggtttcaac gaccccacgg acgtgtaccg ggtggagctc cggaagggag attcgctgaa
      661 ggcgacgctc accggagcgg ccggcagccg cttcgcgctc gggatctgga gcccgcgcac
      721 gcgcggcctc gacatcaccg agaacaggac cagggggcga ctgctcgcgt accgggacaa
      781 gcccggcgcc ggcgagcggc tgaccgcacg aaaagtggcc acgagcggga gttattacgt
      841 gtcggtcgag gcgccgcacc cggatcgcgc cgacaccacg tacgtgttga ccgtccggag
      901 gggctgactt aggatggccc cacaagcggg ctcacctggc ccttctgcgc ccgctggttc
      961 gcttatgcgg tatcgagccg cctacatcgc cggggcgctg gcgatcctgc ttgcaattcc
     1021 cccggcggga gcgggtgctg cgtcgcctgc gccggcgggc ccccacgttc gctcggggca
     1081 tgtgcaggag gatccggcaa cgcgcgcgaa gcgtgccgag cgccggcgca agatacgcag
     1141 gcagctcgcg cgcgaggtgc gacgcaatcc gcgcgtggtg atgacgaaac gcttcatccg
     1201 caaggccggc cacgtgcgct tcgacctgcc gctcaccgtg cggctcaacc cgctgatcga
     1261 cgacgacgtg aacacggcca caccgcccgt tttcgcgccg tccgacgaca cgttcgacta
     1321 cacgacggac gccaccccgg tcgcggatcc cgcgggctcg ctcgcgccgc tcgcaagccc
     1381 gctgctcacg ggcagcttca agctcgccgc gcggttcggg aagacgatcg tcggctacga
     1441 caccggttcg ataggcgtcg aggtcggcac gcgcgacatc gacctgcaag cgggctccat
     1501 ccccctggtg cagccggcga gcccgtgtcc cgactccagt ccggcgctga ggaccgggcc
     1561 gatctcgatc tccgagatca gcggctggtc acgcaccggc ttcctcgcgc tcgcgggcgg
     1621 gcgcacctcg ctcagcctct acacacagtg gagcttcagc tcgctgcgcc aaggcgactt
     1681 cacgaccacg cccggcagtt tcaccgacgg ctgcaccggc cagtacttct ggacgaagaa
     1741 gatcgggccg acgctgccgc cggtaccgct cgagatgatc gggacctcgg cggtgaaccc
     1801 cgcgatcacc acggacggga agctgcggtt cctgaagctc acgatcgacg acgtcgtgct
     1861 gccccaggag gacttcgcgg cgacgattca cgtgtgcagc gacgccgacc cgagcgaaac
     1921 gcctgaggcg agccctccgc ctggcggcat ctgcacgggt gtcagcggcg acgacgtgcc
     1981 gatcgccgcg cggctgaagg tgaagaagct cacagccgaa gtgctgctcg gcgacctgcc
     2041 ctcgtagctg cgagcgtcag gcgcgcaggc tcgacagccg caggtagcgc gagacggcct
     2101 cggcgcggtt ggccgcgcgc agcttgcgca ggatgtgctt cacgtgcgac ttcaccgtgc
     2161 cgggtgagat cacgaggcgc tcggcgatca tcgcgttggt ggcgccttcg gccatcagcg
     2221 cgagcacctc gcgctcgcgg cgcgtgagca gcccctccgt ggggtccgcg aagtcggagc
     2281 cccgcagacg acgcggcgcg atcccctcga cggacgtgcg ctcggcgggg agcgccagct
     2341 gctcgccctc cgactcggcg attgcgctct ccatcgcggc gagcatctcg tgcaggcgcc
     2401 gcgactgcgc gcgcagccgg ttctgcatca gcatgcactc gtacgcgtgg gtgaagccct
     2461 cggcgaaggc ccagatcacc tcacggtcga gcggcgtcgc ctgcgtgcgg gcgaagaagt
     2521 gatcggccgc gaagaagccg acgacgcgtc cattcgccgc tacgggcgcg gcgacataag
     2581 accgcgtccg gcacgcacgc acgatcgcct cgtgcgttcg ctcgtcgtta cgggcgtcct
     2641 caacgagcac cgccttgcgc tggcggatca tgtcggtctc gtggagctcg ggcatgagct
     2701 gcggctcgga atgccgcacc agatcgagga actccgcggc gcggctcggg tgcgcctcca
     2761 tgtgcacact ctccggcgcg atttgcgagc cctgcacgct gaagaagatc gagcggtcga
     2821 agccgcagtg cctgcaaagc tcgaccgtcg ccttcgagag cagctgctcc gtgctgccgg
     2881 cgccgcgcag gcgcgccagc gcttctctca cgccgccaag cgcgccggga acgtcggatt
     2941 tggtctgctc cgcacgcctt ttggtggttt tggccaggcc accgtgtagc gccggagcag
     3001 ccgttgctgc cctcgtgttc aaatcgccct cgagtccgcg ccgatcctaa cacggtgata
     3061 ccccctatgg gggacaacat gtgtaaccgc tagtgcgagg cgctgtaacc gatgcgtcag
     3121 gcgcgcgctg gcgcaagctc cgatggggca agcgcccgct gcggacgccg cgcgcggcgc
     3181 cgcgaacgct tccgctcgcg agccagctca cgcatgtaag ggtggaaatc gacctggatc
     3241 gtgtgacgcg gcgagcgcac gtagcgcttc gccatacgct cgcgat
//
LOCUS       DASVXS010000222         7728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_809001, whole genome shotgun sequence.
ACCESSION   DASVXS010000222 DASVXS010000000
VERSION     DASVXS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809001"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4254
                     /locus_tag="VF752_12340"
     CDS             <1..4254
                     /locus_tag="VF752_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX8856377.1"
                     /translation="VMSRLGLRVLAEREDVVDSSALGATAREEEPVFWQVGGLVEEPR
                     LCFEITVELESELGVHVASFSTHTCVYKVMGTPAVLGAYYPDLHDKRIETAALLGHNR
                     YSTNTWPSFKRVQPFSLLGHNGEINTIARLCQEAEMLGVPIHEDGSDSQDLNRIVEAL
                     INRRGLSLVEAIELVLPPIVNEIKQLPDDLRSFYMYLRQAFGPFAQGPVALLSRHGDE
                     CVFSVDALGLRPLWQVETQGAYVFTSEPGVVPIADVVSEPKPLAPGEKVMVSIERRKR
                     EARLRDHARLQWLAQERWIARTGAEPIHGFGEMIPVGGPLEGAEIPGYTSAGPAEPVK
                     VEDRVLGGFGWQRDDMKLVQQMAATGQEPIGSLGYDGPLACLSPERQNLADYFKESVA
                     VVTNPAIDREREIEHFSCRAVFGRRPRLSELDPVPHTIETAFPIVLGGHDGLAPLSDS
                     VYRAVAKDHKTYLLEDLWEAYRGRAKVLDISCLEAETTQGAIERLRHEATTAARDGSE
                     LIVLSDRTAYEGDRRYIDPHLALSAVDLALRQAWVEPGEQSLRRRCGLVLRSGALRNV
                     HDVVLALGLGADGVCPYVMVEVACMDDYRQDIENLGAALRKGIEKVISTIGIHEVRGY
                     ARLFSCIGLKPELVALLDTPAYFGSDSGGLGFDELDADGAERQRILEGGLESKPAKTF
                     HFYPKVYKAAIAAANGSGGYDEYSEKVRELELEQPISLRHILELKGDREQVAEADASV
                     GLHSYPIVISSMSFGSQGETAYRAYAEAAKRINVIAMNGEGGEIQDMYGKYPKWRGQQ
                     VASGRFGVTSEMVNSSYLVEIKIGQGAKPGEGGHLPAKKVTEKVALARNASPGTDLIS
                     PSNNHDLYSIEDLAELIDELKTANPDVRVSVKVPVVPNIGTIGVGIAKAGADIITLSG
                     FEGGTGAARSHALRHVGLPSDIGTRAVHRALLEAGIRNRVEIWADGGFRHGYDIVKMH
                     CLGANRVGFGTLAMVSLGCTICRGCQLDTCHVGIATQIETKHEADLKGLKKFTPQEFE
                     RAADSCARFFEGMGEEVRRIVASLGYERAQDLVGRSDLLVQAHAKGSVDVTDLVAPLE
                     EFLDLEPIDLPTPADEREEPGLVVARPIRMKPKDASQEVAALATEVVAGTRVTRQYPR
                     PADAKDRVLGTELAGELARARIFQGAPEASEDTLAHIQFDGGSVAGQGLGAFNVYGVD
                     ITVEGGAQDGVAKTMLGGKVSILKGRNRAGRRVNGSVGKSFAYGAQRGRLFVQGNADS
                     RFCIRLSGADVVIGGEPEEPLHDERGGIADRANIKGFAFEYMTAGRAVVLGDPGPWFC
                     AGQTGGRVYLRVNGDWGLTREALAFRKGKGAKVSLEDLDAEGLLDVQELLGYYAEELR
                     QTGQPDEALRILDLAADPRAHFIMSVPQGQQTDPSVSTE"
     gene            4293..4472
                     /locus_tag="VF752_12345"
     CDS             4293..4472
                     /locus_tag="VF752_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856378.1"
                     /translation="MNPNAGAAAAMVLVALFALRFLLFHSRVLGVAHGPARILAGVVF
                     AAALIATRLLARRRH"
     gene            4501..4965
                     /locus_tag="VF752_12350"
     CDS             4501..4965
                     /locus_tag="VF752_12350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8856379.1"
                     /translation="MGERATASKRVFYRVAHRILRVWWRLAHPKTRGVRCVLERDGSV
                     LLVRHTYGSREWSLPGGRMRPWEEAAQTAAREMHEELGLELDGWRELATFQSRNYGKR
                     ETVFFLGADIGGSELTIDAGEIGEVRWASREEAAATRSNSLREAVAAGVLAS"
     gene            complement(5054..5677)
                     /locus_tag="VF752_12355"
     CDS             complement(5054..5677)
                     /locus_tag="VF752_12355"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8856380.1"
                     /translation="MPRFVIIRTITALGESKMQAFMSGTLAGAGSFRCESCGFAVALH
                     ERDEVPSCPRCDGGRFRRASIFEDPTALEPFGPREVEVPVWLAEARDMLAAPGWYLAH
                     DSDDAVSVIPLQDGWTRIGRSLAAHVRFDDPTVSRRHALLHCDEHGARILDDRSLNGV
                     FLNGDRIDWHELDDGDEIVIGRFRLYFLSLTGDEPRAASGEVGTAVG"
     gene            5852..6100
                     /locus_tag="VF752_12360"
     CDS             5852..6100
                     /locus_tag="VF752_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856381.1"
                     /translation="MPNDIASLLLRKSIGALSKSRGSCCSRCRRTPVAGELMHVFESG
                     STLCTLCTAELPEAERRPLRSERVHMTDRHVFAVPRAA"
     gene            6145..6693
                     /locus_tag="VF752_12365"
     CDS             6145..6693
                     /locus_tag="VF752_12365"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8856382.1"
                     /translation="MRPVTVRTHISAPREEVFDLVADMGTRVAWCDHFQGEFRLTRPV
                     TYGQGSAARWRAEAPFNHGYVESAIVEFDRPRRIFEQLRMGRNNRVPGWAEYVFEPHT
                     RGVTRVELTVWTEPQLAVDRLKESFGQRGWTKRRLNTALDRLRKIFEEPSSEPLRRAG
                     VAGYEPWKAPRFGGHIRERGAG"
     gene            6741..7295
                     /locus_tag="VF752_12370"
     CDS             6741..7295
                     /locus_tag="VF752_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856383.1"
                     /translation="MSRRLLRLLAAGVLALALVTTLGACSKKRDFPIREGLGLEVNGL
                     KYTVFITRELNLRDPEDKSYFKGPEAPPGSAYYGVFLQVCNEGSKPLPATRDFTVVDT
                     QGNHFQPIPLPATNDYRYKPDIVAPHRCLPTLGSMAQIGPASGSLLLFKIPNANIENR
                     PLDLQIGASGTQNVSDHQRVELDI"
     gene            complement(7420..>7728)
                     /locus_tag="VF752_12375"
     CDS             complement(7420..>7728)
                     /locus_tag="VF752_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8856384.1"
                     /translation="MIGVVDYGTGTRAQLGGRTKVAGKTGTAELEQTVKRDQPGAPDT
                     QTTPSETKEPPESKTDAWFAAYAGVRHPKLAVSVMLVRAGAGGDTAAPAAKTVILAAL
                     "
BASE COUNT         1187 a         2648 c         2742 g         1151 t
ORIGIN      
        1 gtgatgagcc gtctcggtct gcgcgtgctc gccgagcggg aggacgtggt cgactcgtcg
       61 gcgctcggcg cgaccgcgcg cgaggaggag ccggtcttct ggcaggttgg cgggctggtc
      121 gaggagccgc gcctctgctt cgagatcacg gtcgagctcg agagcgagct gggcgtgcac
      181 gtggcttcgt tctccacgca cacgtgcgtc tacaaggtga tgggcacgcc cgcggtgctc
      241 ggcgcctact acccggacct gcacgacaag cgcatcgaga ccgccgcgct gctcggccac
      301 aaccgctact ccacgaacac ctggccctcg ttcaagcgcg tgcagccgtt ctcgctgctc
      361 ggacacaacg gtgagatcaa caccatcgcg cgtctgtgcc aggaggcgga gatgctcggc
      421 gtgccgatcc acgaggacgg ctcagactcg caggacctga accgcatcgt cgaggcgctg
      481 atcaaccggc gcgggctctc gctggtggag gcgatcgagc tcgtactgcc gccgatcgtg
      541 aacgagatca agcagctgcc cgacgacctg cgctcgttct acatgtacct gcgtcaggcg
      601 ttcggcccgt tcgcgcaggg gccggtcgcg cttctgtcac gccacggaga cgagtgcgtg
      661 ttcagcgtcg acgcgctcgg cctgcggccg ctctggcagg tggagacgca gggggcctac
      721 gtgttcacgt ccgagcccgg cgtcgtgccg atcgcggacg tcgtctcgga gccgaagccg
      781 ctcgcgccgg gtgagaaggt gatggtgtcg atcgagcggc gcaagcgcga ggcgcggctg
      841 cgcgaccacg cgcggcttca gtggctcgcg caggagcgct ggatcgcgcg caccggcgcc
      901 gagccgatcc acggcttcgg cgagatgatc ccggtcggcg gcccgctcga gggcgcggag
      961 atccccggct acacgagcgc cgggccggcc gagccggtga aggtcgagga ccgcgtgctc
     1021 ggcggcttcg gctggcagcg cgacgacatg aagctcgtgc agcagatggc cgccacgggg
     1081 caggagccga tcggatcgct cgggtacgac ggcccgctcg cctgcctctc gcccgagcgg
     1141 cagaacctcg cggactactt caaggagtcg gtcgccgtgg tcaccaaccc cgcgatcgac
     1201 cgcgagcgcg agatcgagca cttctcctgc cgcgcggtgt tcgggcgccg tccgcgcctc
     1261 agcgagctcg acccggtgcc gcacacgatc gagaccgcct tcccaatcgt gctcggcggg
     1321 cacgacgggc tcgccccgct gtcggactcg gtctaccgcg cggtggcgaa ggaccacaag
     1381 acgtatctgc tcgaggacct ctgggaggcg taccgcgggc gcgcgaaggt gctcgacatc
     1441 tcgtgcctcg aggcagagac cacgcagggc gcgatcgagc ggcttcgcca cgaggcgacg
     1501 accgctgcgc gtgacggctc ggagctgatc gtgctctcgg accgcaccgc ctacgagggc
     1561 gaccgccgct acatcgatcc gcacctggcg ctgagcgcgg tcgacctcgc gctgcgacag
     1621 gcatgggtcg agcctggcga gcagagcctg cggcgccgct gcgggctggt gctccggtca
     1681 ggcgccctcc gcaacgtgca cgacgtcgtg ctcgctcttg ggctcggcgc ggacggcgtc
     1741 tgtccctacg taatggtcga ggtcgcctgc atggacgact accgccagga catcgagaac
     1801 ctcggcgcgg cgctgcgcaa gggcatcgag aaggtgattt cgacgatcgg catccacgag
     1861 gtgcgcggct acgcgcggct gttctcgtgc atcgggctga aaccggagct cgtggcgctg
     1921 ctcgacacgc ccgcctactt cggctcggac tcgggcggcc tgggtttcga cgagctcgac
     1981 gccgacggcg ccgagcggca gcggatcctc gagggcggcc tcgaatcgaa gcccgcgaag
     2041 accttccact tctacccgaa ggtctataag gccgcgatcg cggcggcgaa cggctcgggc
     2101 ggctacgacg agtactccga gaaggtgcgc gagctcgagc tggagcagcc gatctcgctt
     2161 cgccacattc tcgagctcaa gggcgaccgc gagcaggtgg cggaggcgga cgcctcggtt
     2221 ggcctgcaca gctacccgat cgtgatcagc tcgatgagct tcggctcgca gggcgagacc
     2281 gcctaccgcg cctacgccga ggccgccaag cgcatcaacg tgatcgccat gaacggcgag
     2341 ggcggcgaga tccaggacat gtacggcaag tacccgaagt ggcgcggcca gcaggtggca
     2401 tcgggccgct tcggggtgac gagcgagatg gtcaactcgt cctacctcgt ggagatcaag
     2461 atcggccagg gcgcgaagcc gggcgagggc ggccacctgc cggcgaagaa ggtcaccgag
     2521 aaggtcgcgc tggcgcgcaa cgcctcaccg ggcaccgacc tgatctcgcc ctcgaacaac
     2581 cacgacctct actcgatcga ggacctcgcg gagctgatcg acgagctgaa gacggcgaac
     2641 cccgacgtac gggtgtcggt gaaggtgccg gtggtgccga acatcggcac gatcggtgtc
     2701 ggcatcgcga aggctggcgc cgacatcatc acgctgagcg gcttcgaggg aggcacgggc
     2761 gcggcgcgct cgcacgcgct gcggcacgtg ggcctgccgt ctgacatcgg cacgcgtgcg
     2821 gtgcaccgag cgctgctcga ggccggcatc cgcaaccgcg tggagatctg ggcggacggc
     2881 ggcttccgcc acggctacga catcgtgaag atgcactgcc tcggcgcgaa ccgagtcggc
     2941 ttcggcacgc tcgcgatggt ctcgctcggc tgcacgatct gccgcggctg ccagctcgac
     3001 acctgccacg tgggcatagc cacacagatc gagacgaagc acgaggccga cctcaagggc
     3061 ctcaagaagt tcacgccgca ggagttcgag cgcgcagccg actcgtgcgc gcgcttcttc
     3121 gagggcatgg gcgaggaggt ccggcgtatc gtcgcgtcgc tcggctacga gcgcgcccag
     3181 gatctggtcg gccgctcgga tctcttggta caggcgcacg cgaagggctc agtggacgtg
     3241 accgacctcg tcgcgccgct cgaggaattc ctcgacctcg agccgatcga cctgccgacg
     3301 ccggctgacg agcgcgagga gcccgggctg gtggtggcac gtccgatccg catgaagccg
     3361 aaggacgcct cgcaggaggt ggccgccctc gcgacggagg tcgtcgccgg cacccgcgtg
     3421 acgcgccagt acccgcgtcc cgccgacgcc aaggaccgcg tgctcggcac cgagctggcg
     3481 ggagagctcg cgcgggcgcg catcttccag ggcgcgcccg aggcgagcga ggacacactc
     3541 gcgcacatcc aattcgacgg tggctcggtc gcgggccagg ggctcggcgc cttcaacgtg
     3601 tacggcgtgg acatcacggt ggagggcggc gcccaggatg gcgtggcgaa gacgatgctc
     3661 ggcggcaagg tgtcgatctt gaagggccgc aaccgggcag ggcggcgcgt gaacggctcg
     3721 gtcgggaagt cgttcgccta cggcgcgcag cgcggccgcc tgttcgtgca ggggaacgcc
     3781 gattcgcgct tctgcatcag gctctctggc gcggacgtcg tgatcggtgg cgagccggag
     3841 gagccgctgc acgacgagcg cggtggcatc gcggaccgcg cgaacatcaa gggcttcgcg
     3901 ttcgagtaca tgaccgccgg ccgggccgtc gtcctgggcg acccgggccc gtggttctgc
     3961 gcgggccaga ccggcggccg cgtgtacctg cgcgtgaacg gcgactgggg cctcacccgt
     4021 gaggcgctcg cgttccgtaa gggcaagggc gcaaaggtgt cgctcgagga cctcgacgcc
     4081 gagggcttgc tcgacgtgca ggagctgctc ggctactacg ccgaggaatt gcggcagacg
     4141 ggccaacccg acgaggcgct gcggatactc gacctggccg cggatccgcg ggcgcacttc
     4201 atcatgtcgg tcccgcaggg tcagcagacc gatccctcgg tctcaaccga gtaattaggt
     4261 tcgctgcaga ttcccgcggt atatccctag gcgtgaaccc gaacgccggc gctgcggccg
     4321 cgatggtgct ggtggcgctc ttcgcgctgc gcttcctgct gttccacagc cgggtgctcg
     4381 gcgtcgcgca cggacccgcg cgcatcctgg cgggcgtggt gttcgccgcg gcgctcatcg
     4441 ccacccggct gctggcgcgc cgccgccact agcgcccgat tcgggcttaa gctcgaacgc
     4501 atgggagaga gggcgaccgc ctccaagcgg gtcttctacc gcgtggctca ccgaatcctg
     4561 cgcgtgtggt ggcggctggc gcacccgaag acgcgcggcg tgcgctgcgt gctcgagcgg
     4621 gacggcagcg ttctgctcgt ccggcacacg tacggctcgc gtgaatggtc gctccccggc
     4681 ggccgcatgc ggccgtggga ggaggcggct cagacggcgg cgcgcgagat gcacgaggag
     4741 ctcgggctcg agctcgacgg ctggcgcgag ctcgccacgt ttcagtcgcg caactacggc
     4801 aagcgcgaga ccgtcttctt cctcggcgcg gacatcggcg gcagcgagct gacgatcgac
     4861 gccggtgaga tcggcgaggt gcggtgggcg agccgcgagg aggcggctgc cacgcgatcg
     4921 aactcgctgc gcgaggccgt ggccgcaggc gtgctcgcct cgtagacgat cgaggccccg
     4981 gcgaacgggg cctcggtcac tcctccctca accggcccac ccctcagggg gcttcgccgg
     5041 cgctcgcggc gggtcagccg acggctgtgc cgacttctcc gctcgccgcg cgcggctcgt
     5101 ctccggtcag gctcaggaaa tacaggcgga atcgcccgat cacgatctcg tcgccgtcgt
     5161 cgagctcgtg ccagtcgatc cggtcgccgt tgaggaacac gccgttgagg ctgcggtcgt
     5221 cgaggatgcg cgccccgtgc tcgtcgcagt gcaggagggc atggcgccgc gagacggtcg
     5281 ggtcgtcgaa gcgcacatgc gccgcgaggc tccggccgat acgggtccaa ccatcctgca
     5341 gcgggatgac gctcacggcg tcgtccgagt catgcgccag gtaccagccg ggggcggcga
     5401 gcatgtcgcg cgcctccgcc agccagaccg gcacctcgac ctcgcgcggg ccgaaaggct
     5461 cgagcgcggt cgggtcctcg aaaatcgacg ctcgccgaaa gcgcccaccg tcacagcggg
     5521 ggcaggacgg cacctcgtcc cgctcgtgga gagctacggc aaagccgcag gactcacacc
     5581 ggaacgaacc cgctccggcg agcgtgcccg acatgaaggc ttgcattttt gactctccca
     5641 gggcggtgat cgtgcggatg ataacgaagc ggggcatggg ttcaacagcc aaaaacccgc
     5701 aaatagcgca ccaattcacg ctgatggcgc acgcccacag ggcttttggg ccatttcgtc
     5761 gaaaagcgcg actatccctc aagcgtggta gatcgcggga ggcttcgtgc agcggggagg
     5821 agtcgggcgc gcgcttcccg aacttcgcgc catgccgaac gacatcgcct ccctcctcct
     5881 gcgcaagagc atcggcgcgc tctcgaagag ccgcggctct tgctgctctc gctgccgccg
     5941 cacgccggtt gccggcgagt tgatgcacgt cttcgagtcg ggcagcacgc tgtgcacact
     6001 gtgcacggcc gagctgcccg aagccgagcg ccggcctctc cgctccgagc gggtgcacat
     6061 gacggaccgg cacgtgttcg cagttccgcg cgccgcctga cgtccgggca gccgccgccc
     6121 gcgcgtccat agaatgcgcg gccgatgcgc ccagtaacgg ttcgcacgca catctccgcg
     6181 ccgcgcgagg aggtcttcga cctcgtcgcc gacatgggca cgcgtgtggc ctggtgcgac
     6241 cacttccagg gtgagttccg gctcacccgg cccgtcacct acgggcaggg ctccgccgcg
     6301 cgctggcgcg ccgaggcgcc gttcaaccat ggctacgtcg agagcgccat cgtggagttc
     6361 gaccgcccgc gccggatctt cgaacagctc cgcatggggc gcaacaaccg cgtgcccgga
     6421 tgggccgagt acgtcttcga gccgcatact cggggcgtga cgcgcgtgga gctcacagtc
     6481 tggaccgagc cgcagctggc ggtcgaccgg ctgaaggagt ccttcggtca gcgcggctgg
     6541 accaagcggc ggctgaacac tgcgctcgac aggctgcgga agatcttcga ggagccgtcg
     6601 agcgagccgc tgcggcgcgc cggcgtggcc ggctacgagc catggaaggc gccccgcttc
     6661 ggagggcaca tccgggagcg gggagcgggg tgatcgcggg caagcgccgg ggccggaccg
     6721 gcggatatat tccgcggccg atgtcccgac gactgctcag gctgctcgcg gccggcgtac
     6781 tcgcgctcgc gctcgtcacg acgctcggcg cgtgctcgaa gaagcgcgac ttcccaatcc
     6841 gcgaggggct tgggctcgag gtgaacggcc tgaagtacac cgtcttcatc acgcgcgagt
     6901 tgaacctgcg cgaccccgag gacaagtcct acttcaaggg gccggaggcg cctccgggct
     6961 ccgcctacta cggcgtgttc ctgcaggtgt gcaacgaggg cagcaagccg cttcccgcca
     7021 cgcgtgactt cacagtcgtc gacacgcagg gcaatcactt ccagccgatc ccgctgccgg
     7081 ccacgaacga ctaccgctac aagccggaca tcgtcgcgcc gcaccgctgc ctgccgacgc
     7141 tcgggagcat ggcgcagatc ggaccggctt cgggctcact gctgctgttc aagattccga
     7201 acgcgaacat cgagaaccgg ccgctcgatc tccagatcgg cgcgtcgggc acgcagaacg
     7261 tctcggatca ccagcgcgtc gagctcgaca tctgactggg gcggctgcaa agcggcgcat
     7321 tgcggcgtcc tcgggcgctc gccgtactgt cgtacggctt cggccctgcg tccttgcact
     7381 gcttgctttt cgcatcccca gtcagatgtc ccggacggtt tagagcgcgg cgaggatgac
     7441 cgtcttcgcg gctggggctg ctgtgtcgcc gccggccccc gcgcgcacga gcatcaccga
     7501 gacggccagc ttcgggtggc gcacgcccgc gtacgcggcg aaccatgcgt cggtcttgct
     7561 ctcgggcggc tccttcgtct cggacggcgt cgtctgcgtg tcgggggcgc caggctggtc
     7621 gcgcttcacg gtctgctcga gctcggcggt cccagtcttg cccgccacct tggtgcgccc
     7681 gccgagctgc gcgcgcgtgc ccgtcccgta gtcgaccacg ccgatcat
//
LOCUS       DASVXS010000223        16881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_417110, whole genome shotgun sequence.
ACCESSION   DASVXS010000223 DASVXS010000000
VERSION     DASVXS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16881
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417110"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VF752_12380"
     CDS             complement(<1..1047)
                     /locus_tag="VF752_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8856385.1"
                     /translation="MPESRYDCIVIGSGPGGYVAAIRAAQLGMKTAVVEKDAIGGRCL
                     NYACIPAKAVLRAAEVLSETEHGKSFGVKVNGVSVDMKGVSAHREKVVKTLTGGVGML
                     FKKNKIDVIEGHGAITDDGNVRIGGQFDGTEIEAGSVILATGSVSKPLLGLQFGGRVL
                     GTETGWALDELPGTLAVIGAGASGTEVASAFGRLGSNVTLLEALPQILPLEDEEIARA
                     AARELKKQNVDVVTEAKIDGAEAKKDGVELTWGGETKKFDYLVIAAGRGPDDEGLGLS
                     EAGVKTTQSGQVEVDGRMRTSLDRVYAIGDLVPVGPALAHKASDEGIIAVEDAAGMET
                     HPIDYMNIPSVTFCY"
     gene            complement(1062..1520)
                     /locus_tag="VF752_12385"
     CDS             complement(1062..1520)
                     /locus_tag="VF752_12385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8856386.1"
                     /translation="MRRDGGGAPAPAERPESAFSVVQRVRFGDLDAMQHMNNVEFLRF
                     FETARIDWIRTLAPQHDPGSRSQLGFIFAECHINYRSPAHYDEELRTYVWPSDIRRSS
                     IRVQFEMRTEHDDRLVADGYGVLVGYDYAEGRPRPLPDGLRERLQVERVG"
     gene            complement(1517..1960)
                     /locus_tag="VF752_12390"
     CDS             complement(1517..1960)
                     /locus_tag="VF752_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856387.1"
                     /translation="MPPEPARVSRSEAKNAAVRDRLEPLRPGERPPAVTIGALVALAL
                     AVTNIAFYAAGAEIDGKRPAFSAIGSYTVILLALAWGMWNAKYLAVLAMQALLGILIL
                     IFAILAIYAANAAAVAVCVVVIAAAGTLFWFLVKALARIQMPERR"
     gene            complement(1950..2603)
                     /gene="thiE"
                     /locus_tag="VF752_12395"
     CDS             complement(1950..2603)
                     /gene="thiE"
                     /locus_tag="VF752_12395"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8856388.1"
                     /translation="MTSDGDVRRERLRAAHLYLVIEALPDVVERALAGGVDVVQLRDK
                     ELDDEELHDAALELRGLCEAAGALFIVNDRPEIALQAGADGVHVGQDDVPCPEARRTV
                     GPDLLVGLSTHTPVQVYEARSEPVDYIGVGPIYETATKPGRPAVGHDLIGYADAHGQK
                     PFFAIGGIDRTNVAEVARAGASRIAVVRAIRDADDPAAAAAELRGAVLEPWRQAGAA"
     gene            complement(2634..4337)
                     /locus_tag="VF752_12400"
     CDS             complement(2634..4337)
                     /locus_tag="VF752_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856389.1"
                     /translation="MAPRLLAAPMACALALVAGPGTAAANPVQQSIIQDEAHMLYSGQ
                     KVQDQTLDEAKTLGADIVKVTVNWARIAPSGSKKPAGFVGSDPGEYAPANWVPYDNLV
                     RGAAARGLKIFFLVGGRAPAWAVQKGGTAQGSYKPDPVAFGNFVKALGIRYSGNYTPT
                     GQEAPTPDTGGGGGGGGTPACPPGQQWDPQLNQCVVPISSGSAISLFAHSSQASGPLP
                     KVTLWSIWNEPNLNGWLQPQWLNRKVPASPAVYRGLLLAAHDSLVQTGHAGDELLIGE
                     LLPFIRGKSDYPSRVSPLQFLRELACVDKNYKPYRGSAAAARGCSKYKPLPGTGIAHH
                     PYTLKGPDVTDARPDDVAIMELGRMNAALKKLTRAHRFEHRSMPIWLTEFGYQTNPPD
                     PYAFPAKLVPRYMGMSEWFAFKNRLVKSYAQYPLRDDPVDGRGPQRFHGFQSGIRSSV
                     GRLKRGVYQAYEYPFFVRQASKTSVEIFGGVRAGKPGDKIVIQSKAPGSSSYFGIPGG
                     TLKLGPGGYFDKTFRIRNAGLRSFRFVLAVDDSKVSMALAPFARPPASVILPPPGTKK
                     K"
     gene            4471..4707
                     /locus_tag="VF752_12405"
     CDS             4471..4707
                     /locus_tag="VF752_12405"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HEX8856390.1"
                     /translation="MGIGTSIFLIAAGAILKYAVTGHVSGVDIQTVGLILMIVGILGL
                     IISLLYLAIWSDRTRGRGAYTDDVPPRPRDPTRY"
     regulatory      4742..4854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4906..6033
                     /gene="thiO"
                     /locus_tag="VF752_12410"
     CDS             4906..6033
                     /gene="thiO"
                     /locus_tag="VF752_12410"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX8856391.1"
                     /translation="MVVGAGIIGLASAWRAAERGLSVLVLERDVEGAGASGVAAGMLA
                     PVTEALFGEEPLLRLNLESGRMWESFDGELREASGGLPTHRDSKGALVLAADRDDAEE
                     LRRLHAFQRELGLDTEWLSGRECRRLEPGLSPRVPGGILAPHESQVDPAATVTALAAA
                     LEHAGGEIRRGEAAAELLHDGEHVQGVRTGSGATVESEHVLLAAGCWSAQLARVPPEA
                     LPPVRPVKGQILSLHSRGGEPLIRRMVHTPRCYMVARPDGRVVIGATVEERGFDTAVT
                     ADGVYRLLEAAWEVLPDIGELEFVGAQTGLRPGTPDNAPVIGAAGPDGLLVATGHYRN
                     GVLLAPITAEAIAALAAGDEPPAQVAPFSPARFGAVAGGAR"
     gene            6030..6236
                     /gene="thiS"
                     /locus_tag="VF752_12415"
     CDS             6030..6236
                     /gene="thiS"
                     /locus_tag="VF752_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX8856392.1"
                     /translation="MKVLLNGEPVELADGTTVAGAVSASGAEPSGRGIAVAVNGEVVP
                     RGEWASIELTEGERVEVLQAVQGG"
     gene            6229..7002
                     /locus_tag="VF752_12420"
     CDS             6229..7002
                     /locus_tag="VF752_12420"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX8856393.1"
                     /translation="MADPLRIGGRELGSRLIIGTGGFRSLETMGEAVKASGAELATLA
                     LRRVDPSARGSIVDVLQSAGVSLLPNTAGCFTARDAVQTARLARDAFETDWVKLEVIG
                     DDKTLLPDPVELLEAAETLVNDGFTVLPYTNDDPILARRLEDAGCAAVMPLGSPIGSG
                     MGINNPYNLRLILEQARVPVILDAGIGTASDAVLAMEAGCDGVLLASAISRAENPARM
                     AHAMRLAVEAGRLAHESGRIPRRLFAEASTPMEGVPELT"
     gene            6999..7472
                     /locus_tag="VF752_12425"
     CDS             6999..7472
                     /locus_tag="VF752_12425"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8856394.1"
                     /translation="MSEELAEAWAAAWRGEGTFEDCCTADISYEDPVAVNPLAGAHAL
                     SEHAGRLRAAFPDLRVERTAPVLADADARHACIAWRAVGTHKEDLGALPATNRFVVLQ
                     GVHLVELDDGRVRRARGFFDLYEAGIQLGLLPQRGSAAESALLLLRGFGLRPRAT"
     gene            complement(7465..7896)
                     /locus_tag="VF752_12430"
     CDS             complement(7465..7896)
                     /locus_tag="VF752_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8856395.1"
                     /translation="MNRWLALLASTAYLALVAAGCGGGGGNNNSGNTGGGTTSAQQST
                     QTTSSSSGGGGGKTVKVVMKNIQFSPKDVTVTKGGTVEWTNDDSVSHDVTKSGGPGPQ
                     FASGTGNLQQGATYKQTFNTPGKITYVCTVHPGMEGTITVK"
     gene            complement(7955..8863)
                     /gene="argF"
                     /locus_tag="VF752_12435"
     CDS             complement(7955..8863)
                     /gene="argF"
                     /locus_tag="VF752_12435"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX8856396.1"
                     /translation="MPRHFLTGGELTSDELDALLRRAEQLKRERLASRALSGRKVALI
                     FQKPSTRTRVSFEVGVVELGGHPVVLREGEMQLSRGESAPDTARVLSRFVDAIAIRTG
                     PQAIVDELAEHSSVPVINMLTDEHHPCQALADLLTLRERFGTLEGLRLVYLGDGNNVA
                     HSLMVLGAIAGVEVVVAAPAELAPDENVARMARELASNGATVELTTDALAAATGAHAL
                     YTDVWVSMGDSDSDERRRLLQPYRLDERLMEAAREDAIALHCLPAHPGEEITETVLYG
                     ARSAVFDQAENRLHAQKALLEFLITE"
     gene            8852..9685
                     /locus_tag="VF752_12440"
     CDS             8852..9685
                     /locus_tag="VF752_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8856397.1"
                     /translation="MARHSGIVIRALSWNLFHGRDAPPDPALFTSRSRLLRVTERDDT
                     HVQVNRRLLREFAATLAGWQWDIALLQEAPPGWLRPLARAAGASGASVRTSRNTGHAL
                     RALIARWNPDLVASHEGGSNQLLARPPWRLAEVRRQTLTLLPERRRMIWARLEHPEHG
                     DLCVANLHATAHVPESAERDVRKAAVTAVRWSGDLPLVFGGDMNLRPNRTDLFDWLAR
                     EHGFSQPTIPNAIDHVLTRGLEVAEPPRRLPPEARELRQPCGRALRLSDHAPVVAAFM
                     R"
     gene            complement(9675..9947)
                     /locus_tag="VF752_12445"
     CDS             complement(9675..9947)
                     /locus_tag="VF752_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856398.1"
                     /translation="MAAALRLVLPPREDDDARAEVERDDDDLDEDERDDDARPPDERL
                     LEERLLDAELRDLPLDDPLLPCAIACSSSRAFAPIPATEDLAGTIS"
     gene            10114..10575
                     /locus_tag="VF752_12450"
     CDS             10114..10575
                     /locus_tag="VF752_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856399.1"
                     /translation="MLTRDRIEEVLGDAVSRGRVTSDDAQELVQGLVQRGRKETNEVL
                     RDLEQLMGGSDLRKRGVDTASRARRQVGDATARVRRAADPVIAQADRARRTAGVGSNF
                     PIAGYADLSVAQVRSRLGSLTPAELRKVRDYERRNANRKTVLNDIEQRLNA"
     gene            complement(10669..12123)
                     /locus_tag="VF752_12455"
     CDS             complement(10669..12123)
                     /locus_tag="VF752_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856400.1"
                     /translation="MDLSAYREEAEAFVSALDREYYLHFSGQKDELEIEPIYERHAGL
                     VTREAVERLRESGNRELLDFAAKGLIGQETKHLDAELARREAALEIEVDGERMPVRQA
                     PVVQANEPDRERRARIDAAQRELLERELQQLHRESFERTSELVRELGWRSVRAMTEAL
                     SGIDLAALADQTEAFLRDTEPSYEPLVEPRLREELGYGFDRFQRADIGAFFRAPALDG
                     AFPAERLLERYRHTMSTMSVDGRGVLLDVDERPKKAPRAFCSPARVPDEVYLVIAPHG
                     GREDYETLFHEAGHAQHYSHVDAALPFEDRYLGDNSVTESFAFLFQSLSADPSWLRDA
                     LGVASPDEVVEQARAAKLVLVRRYCAKLSYELELQDAPADLDALPDVYRRRLSDAVHV
                     DWPRETWLSDVDPFFYSARYLRAWALETHLRRHFRERFGEAWWSSPEAGEQLRSLWRY
                     GQRLNGDELLAELTGGDAQLDFSAMLAEFAEQQQ"
     gene            12145..13503
                     /gene="rlmD"
                     /locus_tag="VF752_12460"
     CDS             12145..13503
                     /gene="rlmD"
                     /locus_tag="VF752_12460"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX8856401.1"
                     /translation="MTQQQTGARPKQGDELDLTIESLAHGGNGVARTEGYVVFVKGAV
                     PGDRVRARVEKRKRAYAEARAVELLEPSPERVEPRAPHPGAPWQVLPYERQLEEKHSQ
                     VEDALTRLGGFSDPPVEPIVPAVEQWRYRNKLEYSFGEDESGRLVLGFHRPGRWNDID
                     DVTEDILASERIDGVRERVKEWCRDEGLSAYDRRDNTGFLRNLVVREGRRTGQLQVRV
                     VTSPGEFNADGLAAVLEDAHGVLWTQTEGVAETTREGSTKLLRGKKTIEDEICGLRFR
                     ISPEAFFQTNTEMAEILYGTAMELAGLSGRERVFDLFSGIGTIALAMSTEAREVFGVE
                     VIEQAVADAIQNARLNGVDNARFFAGDVRLAMRELVEEAGRPDVLVVDPPRAGLSAKI
                     VRRILGAEPTRIVYVSCNPTTLAPNARQMVEGGYRLDAVRPVDMFPQTPHIECVAALS
                     RA"
     gene            13536..14720
                     /locus_tag="VF752_12465"
     CDS             13536..14720
                     /locus_tag="VF752_12465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8856402.1"
                     /translation="MRDSRAAARIVDVSDVRFRVGTIKIGVGITLAVCSVGIAYAIAT
                     WNHANRSLIVVLLALAWLSAWVIALMDAEKIVRSRWREPFFFLWSALDVAIVGALEAA
                     DGGARSPTVLIYVLPLAFAALSYPLPSVVAISLLDVITYVAVSAAADPPGFTYIAFVA
                     ASLACAAGLCVAQARNHHAQRAELGRVSRTDPLTEALNRRGFEERLDAALAEAHRERT
                     PLALLLLDLDNFKLVNDTRGHAAGDELLCWVVATVRATLRPVDALGRLGGDEFAVVLP
                     GANRAEALEVAERVERTLAPRVQATIGLAAFPADGSEREELHRHADNDLYGAKHERAI
                     ARESAPREVAQPANRADVTAYARAIAKQLGLEGDELSRVLTAAEELEAHLAAAVQQKR
                     IA"
     gene            complement(14717..14896)
                     /locus_tag="VF752_12470"
     CDS             complement(14717..14896)
                     /locus_tag="VF752_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856403.1"
                     /translation="MDLEEHTGVEPVIERTCAECGAKLTDQEIQASLDSGGPFLCSVH
                     AAEEVPLGEEDALEP"
     gene            complement(14932..15360)
                     /locus_tag="VF752_12475"
     CDS             complement(14932..15360)
                     /locus_tag="VF752_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856404.1"
                     /translation="MTNVLVVANRTAESPELLEALKQRAADGDVKFTLLVPATPHGVA
                     WAADMHSGGEEAEQHVANAVERLRQAGLTVEGKAGDPDPVAAVEDAVNFDKFDEVICS
                     TLPVHVSKWLKLDLPHRVERVTGLPVQHVVASDSTVPAGG"
     gene            complement(15427..16038)
                     /locus_tag="VF752_12480"
     CDS             complement(15427..16038)
                     /locus_tag="VF752_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8856405.1"
                     /translation="MRVPATDDEIREKYLERAIRELNQLTRELQDCQYCPRGNLMPVL
                     GSGHPQADIFLVKYAPMPSEIEEGVAFYGRSGSALMKSFKRLAIDPLAVYGTLFVKCP
                     VHDTEDSAPECRARVLDELTIVQPRIVVAMGEDALDELNELGVPLSRPVEARPGDLQR
                     LTPAMDFLYVPNIDSALDEESAKRSFWRAFRSLGDWYADLPPY"
     gene            complement(16101..>16881)
                     /locus_tag="VF752_12485"
     CDS             complement(16101..>16881)
                     /locus_tag="VF752_12485"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX8856406.1"
                     /translation="AVYGRLVRRRHDGRGDPALRSQLQSRPLVDVDLHMHTDHSHDCA
                     TPVESLLDTAARVGLGAIAVTDHNEISGALEAREHTNGRVKVIVGEEVKTADQGEVIG
                     LFIEEKIPRGMTLAETIAEIRRQGGLVYVPHPFDRMHSVPDYEHLLGVVGDIDAIEVF
                     NPRVAFSAFNEEAARFAAKYRIVAGAGSDSHVVQGLGSVKIRMRDFDGPEEFLESLRD
                     ADIVRKKQSLLYVQALKFLQTKARPSQSRRRAGRGASAPQD"
BASE COUNT         2409 a         5917 c         5951 g         2604 t
ORIGIN      
        1 gtagcagaag gtcacgctcg ggatgttcat gtagtcgatc gggtgggtct ccatcccggc
       61 cgcgtcttcg acggcgatga tgccctcgtc ggatgccttg tgggcgagcg ccggcccgac
      121 cggcaccagg tcgccgatcg cgtagacgcg gtcgagcgag gtgcgcatgc gcccgtcgac
      181 ctcgacctgg ccgctctgcg tggtcttgac gccggcctcg gagaggccga ggccctcgtc
      241 gtccggtccg cgcccggcgg cgatgacgag gtagtcgaac ttcttggtct cgccgcccca
      301 ggtgagctcg acgccgtctt tcttcgcctc tgcgccgtcg atcttcgctt cggtgaccac
      361 gtccacgttc tgcttcttca gctcgcgcgc ggcggctcgc gcaatctcct cgtcctcgag
      421 cggcaggatc tgcggcagcg cctcgagcag cgtcacgttc gagccgaggc ggccgaatgc
      481 ggacgccacc tcggtgccgg acgcgcccgc gccgatcacg gcgagagttc cgggcagctc
      541 gtccagcgcc cagccggtct ccgtgccgag cacgcggccc ccgaactgca gcccgagcag
      601 cggcttggaa accgagccgg tcgccaggat cacggatcct gcctcgatct cggtgccgtc
      661 gaactgcccg ccgatgcgca cgttgccgtc gtccgtgatg gcgccgtggc cctcgatcac
      721 gtcgatcttg ttcttcttga acagcatgcc gacgccaccg gtgagcgtct tgacgacctt
      781 ctcgcggtgg gcgctcacgc ccttcatgtc cacggagacg ccgttcacct ttacgccgaa
      841 gctcttgccg tgctccgtct cggagagcac ctcggccgcg cgcagcacag ccttggccgg
      901 gatgcacgcg tagttcaggc agcggccgcc gatggcgtcc ttctcgacca cggcggtctt
      961 cattccgagc tgggcggcgc ggatcgccgc gacgtacccg ccggggcccg atccgatcac
     1021 gatgcagtcg tagcgtgact caggcatgac ggcggtgcag cctatccaac gcgctcaacc
     1081 tgcaatctct cccgcagccc atcgggcagt ggtcgggggc gaccctccgc gtagtcgtag
     1141 cccacgagca cgccgtagcc gtcggccacg agccggtcgt cgtgctcggt ccgcatctcg
     1201 aattgcacgc ggatgctcga gcgcctgatg tccgacggcc acacgtacgt acgcagctct
     1261 tcgtcgtagt gcgccggcga gcggtagttg atgtggcact cggcgaagat gaagccgagc
     1321 tggctgcggc tgccggggtc gtgctggggc gcgagcgtgc gaatccagtc gatgcgcgcc
     1381 gtctcgaaga agcgcaggaa ctcgacgttg ttcatgtgct gcatcgcgtc gaggtcgccg
     1441 aagcgcacac gctgcaccac cgagaaagcg gactccgggc gctctgcggg cgccggagct
     1501 ccgccgccgt cgcgcctcac cggcgctccg gcatctggat gcgggcgagc gccttgacga
     1561 ggaaccagaa cagcgtgccg gccgcggcga tcaccaccac gcacacggcc accgccgccg
     1621 cgttcgccgc atagatcgcg aggatggcga agatcaggat caggatcccg agcagcgcct
     1681 gcatggcgag caccgcgagg tacttcgcgt tccacatgcc ccacgcgagg gcgagcagga
     1741 tcaccgtgta ggagccgatc gcgctgaacg ccggccgctt gccgtcgatc tctgcgcccg
     1801 cggcgtagaa ggcgatgttc gtgacggcga gcgcaagcgc gaccagcgcg ccaatggtca
     1861 ccgccggcgg gcgctccccc ggacgcagcg gctcgagccg atcgcggacg gcggcgttct
     1921 tcgcctcgga acgcgacacg cgcgcgggct caggcggcac cggcctgcct ccagggctcg
     1981 agcacggcgc cgcgaagctc ggcggccgcg gcggccgggt cgtcggcgtc gcggatcgcg
     2041 cgcacaaccg cgatgcgcga ggcgccggct cgggcgacct cggccacgtt cgttcggtcg
     2101 atgccgccga tcgcgaagaa cggcttctgc ccgtgcgcgt ccgcgtagcc gattaggtcg
     2161 tggcccacgg ccgggcgacc cggcttcgtg gcggtctcgt agatcggccc gacgccgatg
     2221 tagtcgaccg gctcggagcg cgcctcgtac acctgcacgg gcgtgtgcgt cgagaggccc
     2281 acgagcaggt cgggcccgac cgtgcgccgc gcctccgggc agggcacgtc gtcctggccc
     2341 acgtgcacgc cgtcggcgcc cgcctgcagc gcgatctcag gccggtcgtt cacgatgaac
     2401 agcgcgccgg cggcctcgca caggccgcgc agctcgagcg cggcgtcgtg aagctcctcg
     2461 tcgtcgagct ccttgtcgcg cagctgcacc acgtccacgc cgcccgcgag cgctcgctcg
     2521 accacatccg gcagcgcctc gatcacgagg tataggtgcg cggcgcggag ccgctcacgg
     2581 cgcacgtcgc cgtcagaggt cacggcaccg atcttggcag accggccgag gcgctacttc
     2641 ttcttggtgc cgggcggcgg caggatcacg ctcgccggcg ggcgggcgaa cggcgcgagc
     2701 gccatgctca ccttcgagtc gtcgaccgcg agcacgaacc ggaagctgcg caggccggcg
     2761 ttgcgaatgc ggaacgtctt gtcgaagtag ccgccggggc cgagcttgag cgtgccgcct
     2821 gggatcccga agtacgacga actgccgggc gccttcgact ggatcacgat cttgtcgccg
     2881 ggcttgcccg cgcgcacgcc gccgaagatc tcgacgctcg tcttgctcgc ctggcgcacg
     2941 aagaacgggt actcgtacgc ctggtagacg ccgcgcttca gccgtcccac ggaggagcgg
     3001 atccccgact ggaagccgtg gaagcgctgc ggaccgcggc cgtcgaccgg atcgtcgcgg
     3061 agcgggtact gcgcgtagct cttcacgagc cggttcttga acgcgaacca ctcgctcatg
     3121 cccatgtagc gaggcacgag cttcgcaggg aacgcgtagg gatccggcgg gttcgtctga
     3181 tagccgaact cggtgagcca gatcggcatg ctgcggtgct cgaagcggtg ggcgcgcgtg
     3241 agcttcttga gagcggcgtt catgcgcccg agctccatga tcgccacgtc gtcgggccgc
     3301 gcgtcggtca cgtcgggacc cttgagcgtg tacgggtggt gggcgatgcc ggtgcccggc
     3361 aggggcttgt acttcgagca cccgcgcgcg gccgcggcgc tgcctcggta cggcttgtag
     3421 ttcttgtcca cgcaggcgag ctcgcggaga aactgcagcg gcgacacgcg cgacgggtag
     3481 tcgctcttgc cgcggatgaa gggaagcagc tcgccgatca gcagctcgtc gcccgcatgg
     3541 ccggtctgca cgaggctgtc gtgcgccgcg agcagcagtc cccggtacac cgcgggcgac
     3601 gccggcacct tgcggttgag ccactgcggc tgcagccagc cgttcaggtt cggctcgttc
     3661 catatcgacc agagcgtgac cttcggcagc gggccggagg cctgggagct gtgggcaaac
     3721 agtgagatcg cgctaccaga cgagatcggc acgacgcact ggttgagctg cgggtcccac
     3781 tgctgtccgg gcgggcacgc cggcgtccca cccccgccgc caccgcctcc tgtgtcaggc
     3841 gtgggagctt cctggccggt cggcgtgtag ttgccggagt agcggatgcc gagcgccttc
     3901 acgaagttcc cgaaggcgac tgggtccggc ttgtacgagc cctgcgcggt accgcccttc
     3961 tgaaccgccc aggccggcgc ccggcccccg acgaggaaga agatcttgag cccgcgcgcc
     4021 gccgcgccac gcacgaggtt gtcgtagggc acccagttcg ccggcgcata ctcgcccggg
     4081 tcggatccga cgaacccggc cggcttcttg ctgccgcttg gcgcgatcct cgcccagttc
     4141 accgtgacct tcacgatgtc ggcgccaagc gttttcgctt cgtcgagcgt ctggtcctgc
     4201 accttctggc ccgaatagag catgtgggcc tcgtcctgga tgatcgactg ctggacggga
     4261 ttcgcggccg cggtccccgg accggcgacg agcgcaaggg cgcacgccat gggcgccgcc
     4321 aggaggcgtg gagccatgga gcgagtcttc atcgagggcc ggatggtaat tgcgcaggac
     4381 gcggcggggc gcgagttccg ttgtaacccg cttgcgggcg accagttgcg gagttttgcg
     4441 cctgccccct ggcgggtacc tcaggtcccg atgggcatag gcacatccat tttcctgatc
     4501 gcggctggcg cgatcctcaa gtacgcggtc acgggccacg tgagcggagt cgacatccag
     4561 acggtcggac tgatcctgat gatcgtcgga atcctcggcc tgatcatctc gctgctgtac
     4621 ctggcgatct ggtccgatcg cacccgcggg agaggcgcct acacggacga cgtcccaccg
     4681 cgcccgcgcg accccacccg ctactagccg ccggacttct cctccgcgag gagtagcctt
     4741 ctcccttgta aacgggagct cggaacggac cgggctgaga gggcggctgg gccgtcgccg
     4801 accgtcgaac ctgatccggg taatgccggc gaagggactc ggcgtgcggc ccccgcggga
     4861 acaggagcca acacgcaagc gccgcagaga gatcgcttcg acctcgtggt cgtgggggcc
     4921 gggatcatcg gcctggcatc cgcctggcgc gccgcggagc gcggcctgtc ggttctcgtg
     4981 ctcgagcgcg acgtggaggg cgccggcgcc tcgggcgtgg cggcgggcat gctcgccccc
     5041 gtcactgagg cgctgttcgg cgaggagcct ctgctgcggc tcaacctcga gtccgggcgc
     5101 atgtgggaga gcttcgacgg cgaactgcgc gaggcctcgg gcggactccc cacccaccgc
     5161 gactccaagg gcgcgctcgt gctcgcggcc gaccgcgacg acgcggaaga gcttcgccgt
     5221 ctgcacgcct tccagcgcga gcttggtctc gacacggagt ggctcagcgg gcgcgagtgc
     5281 cggcggctcg agcccgggct gtcgccgcgc gtgcccggag gaatcctcgc gccgcacgag
     5341 tcgcaggtcg acccggcggc gaccgtgacc gcgcttgccg ccgcactcga acacgcgggt
     5401 ggcgagatcc gtcgcggcga ggcggccgcc gagctgttgc acgacggcga gcacgtacaa
     5461 ggcgtgcgca cgggttccgg cgcgaccgtc gagtccgagc acgtgctgct ggcggcgggg
     5521 tgctggagcg cacagctcgc gcgcgttccg cccgaggcgc tgcccccggt gcgccctgtg
     5581 aagggccaga tcctctctct ccacagccgc ggcggcgagc cgctgatccg gcggatggtg
     5641 cacacgccgc gctgctacat ggtcgcgcga cctgacggcc gtgtggtgat cggcgcgacg
     5701 gtggaggagc gtggcttcga cacggcggtc acggccgacg gcgtctaccg gttgctcgag
     5761 gccgcgtggg aggtgctgcc cgacatcggc gagctcgagt tcgtgggtgc gcagacgggg
     5821 cttcgcccgg gtacgcccga caacgccccc gtgatcgggg cggccgggcc cgacggcctg
     5881 ctggtcgcaa ccgggcacta ccgcaacggc gtgctgctgg cgccgatcac cgccgaggcg
     5941 atcgcagcgc tcgccgctgg cgacgagcct ccggcgcagg tggcgccgtt ctcgccggcg
     6001 cggttcggcg cagtggcggg gggtgcgcga tgaaggtgct tttgaacggc gagcccgtcg
     6061 agctggccga cggcaccacc gtggcgggcg ccgtgagcgc ctccggcgcc gagccatcgg
     6121 gacgcggcat cgcggtggca gtgaacggcg aggtggtgcc gcggggcgag tgggcgtcga
     6181 tcgagctcac cgagggagaa cgcgtcgagg tgctccaggc ggtgcagggt ggctgatccg
     6241 cttcgcatcg gcgggcgaga gctcggctcg cggctgatca tcggcaccgg cggcttccgc
     6301 agcctggaga cgatgggaga ggcggtgaag gcgtcgggcg cggagctcgc cacgctcgcg
     6361 ctccgccgag tggacccgag cgcccgcggc tcgatcgtcg atgtgctcca gagcgcgggc
     6421 gtgagcctgc tcccgaacac ggcgggctgc ttcacggcgc gcgacgcggt ccagaccgcg
     6481 cggctcgccc gggacgcgtt cgagaccgac tgggtgaagc tcgaggtgat cggcgacgac
     6541 aagacgctgc tgcccgaccc cgtcgagctg ctcgaggccg ccgagacgct cgtgaacgac
     6601 ggcttcaccg tgctgccgta caccaacgac gacccgatcc tcgcgcgccg cctcgaggac
     6661 gccggctgcg ccgcggtgat gccgctcggc tcgccgatcg gcagcgggat ggggatcaac
     6721 aatccctaca acctccgcct gatcctcgag caggcgcgcg tgcccgtgat tctcgacgcc
     6781 ggcatcggca ccgcctccga cgcggtgctc gcgatggagg cgggctgcga cggcgtgctc
     6841 ctcgcgagcg cgatctctcg cgccgagaac cccgcgcgca tggcgcacgc gatgcggctc
     6901 gcggtcgagg ccgggcgcct cgcgcacgag tccggccgca tcccgcggcg gctgttcgcc
     6961 gaggcctcca cgcccatgga gggcgttccg gagctcacgt gagcgaggag ctcgcggagg
     7021 cctgggccgc ggcgtggcgg ggcgagggga cgttcgagga ctgctgcacc gcggacatct
     7081 cgtacgagga tccggtggcg gtgaacccac tggccggggc gcacgccctc tccgagcacg
     7141 ccgggcggct acgcgccgcc ttcccggatc tgcgagtcga gcgcacggcg ccggtgctcg
     7201 ccgacgccga cgccaggcat gcctgcatcg catggcgcgc ggtcggcacg cacaaggagg
     7261 acctcggcgc gctcccggcc acgaatcgct tcgtggtgct ccagggcgtc cacctggtgg
     7321 agctcgacga cggccgcgtg cgcagggcgc gcggcttctt cgacctctat gaggccggca
     7381 tccagctcgg gctgctcccg cagaggggca gcgcggctga atccgcgcta ctgctgctgc
     7441 gcggattcgg cctgcgcccg cgcgctactt gacggtgatc gtgccctcca tgccggggtg
     7501 cacggtgcac acgtacgtga tcttgcccgg cgtgttgaag gtctgcttgt aggtggcgcc
     7561 ctgctggagg ttgcccgtgc cggaggcgaa ctgcgggccg gggccgcccg acttcgtcac
     7621 gtcgtgagag acggagtcgt cgttcgtcca ctccaccgtg ccgcccttcg tgacggtcac
     7681 gtccttcggc gagaactgga tgttcttcat gacgaccttg acggtcttgc cgccgccccc
     7741 gccgctgctc gacgaagtgg tctgggtcga ctgctgagcg gaggttgtgc cgccgcctgt
     7801 gttgcccgag ttgttgttac cgccgcctcc accgcaaccg gccgccacaa gcgccagata
     7861 ggcggttgag gcaagcagcg ccagccacct gttcatctgc gtccgacctc ctgtcaggga
     7921 gctttgtcag gagagactac ggcgagcagt gttttcattc agttatgaga aattcgagca
     7981 gcgccttctg cgcgtgaagc cggttttcgg cctggtcgaa caccgccgag cgcgcgccgt
     8041 agagcacggt ttcggtgatt tcctcccccg ggtgagcggg caggcagtga agcgcgatcg
     8101 cgtcctcgcg cgccgcctcc atcagccgct cgtcgagccg gtagggctgg agcagtcgcc
     8161 gccgctcgtc gctgtcggag tcgcccatgc tcacccatac gtcggtgtag agcgcgtgcg
     8221 ctccggtggc cgcggccagc gcgtccgtgg tcagctcgac cgtcgcgccg ttgctcgcca
     8281 gctcacgcgc catgcgggcg acgttctcgt ccggcgccag ctccgccggc gccgcgacga
     8341 ccacctctac gccggcgatg gcgccgagca ccatcagcga gtgcgccacg ttgttgccgt
     8401 cacccaggta cacgaggcgc agtccctcga gcgttccgaa gcgctcgcgc agagtgagca
     8461 ggtccgcgag cgcctgacag gggtggtgct cgtcggtgag catgttgata acgggcacgc
     8521 tcgagtgctc ggcgagctcg tccacgatgg cctgcggccc cgtccgaatc gcgatggcgt
     8581 cgacgaagcg cgacagcacg cgcgccgtgt cgggcgccga ctcgccgcgc gagagctgca
     8641 tctcgccctc tcgaagcacg accgggtggc cgccgagctc gaccacgccc acctcgaacg
     8701 acacccttgt gcgtgtcgat ggcttctgga agatgagcgc gaccttgcgc ccgctcaggg
     8761 cgcgcgacgc gagtcgctcg cgcttgagct gctcggcgcg gcgtagcagg gcatcgagct
     8821 cgtcgctcgt aagctctccg ccggtgagga agtggcgcgg cattcaggca tcgtaattcg
     8881 cgcgctcagc tggaacctct ttcacggccg cgacgctccg ccggaccccg cgctgttcac
     8941 gtcgcgatcg cgtctcttgc gcgtcaccga gcgcgacgac acccacgttc aagtgaaccg
     9001 ccgtctgctc cgcgagttcg ccgccacgct cgcgggctgg cagtgggaca tcgcgctgct
     9061 tcaggaagcg ccgcccggct ggctgcgtcc gcttgcgcgc gcggcgggtg cgtccggcgc
     9121 gtcggtccgg acctcgagga acacggggca cgcgctgcgg gcgctgatcg cccgctggaa
     9181 tcccgacctg gttgcatcgc acgagggcgg ctcgaaccag ctcctcgcgc gcccgccgtg
     9241 gcggctcgcg gaggtgcgac ggcaaacgct cacgctcctg cccgagcggc gccggatgat
     9301 ctgggcgcgc ctcgagcatc cggagcatgg cgatctctgc gtcgccaacc tccacgcgac
     9361 cgcgcacgtg ccggagtctg ccgagcggga cgttcgcaag gcggcggtca ccgcggttcg
     9421 ctggtcggga gaccttccgc tcgtgttcgg cggggacatg aatctgcgcc cgaaccggac
     9481 ggacttgttc gactggctgg cacgcgagca cggcttctca cagccgacca ttccgaacgc
     9541 gatcgaccat gtgctcacac gtgggctgga ggtcgccgag ccgccccgcc gactcccgcc
     9601 ggaggcgcgc gaactgcgcc aaccgtgtgg gcgcgcactg cgtctgtcgg accatgcacc
     9661 ggttgtggcc gcgttcatga gatagtgccc gccagatctt ctgtcgcggg aattggggcg
     9721 aaagcccggg aggaggagca cgcaatggca cagggaagca gcgggtcgtc cagaggcagg
     9781 tcgcgtagct cggcgtcgag caagcgctcc tcgagcagcc gctcgtcggg tggccgcgcg
     9841 tcgtcgtcgc gttcatcctc atcgaggtca tcatcgtcgc gctctacctc cgcacgggcg
     9901 tcatcgtctt cgcgcggcgg cagcaccaag cgcagcgccg cagccaagaa gggcggtcag
     9961 gcgcgcgggc gtcagcagac cgcgcagaag cgcgcgcgcc agacggcttc ggcggctacc
    10021 aagccggccg ccgcggcgga cttctcgggc aagagcgtcg ccgatttccg cgaagccctc
    10081 accaagaacc tcatccgccc gctcaacatc gtgatgctca cccgcgatcg catcgaggaa
    10141 gtgctcggcg acgccgtgtc gcgcggacgc gtgacctcgg acgatgcgca ggagctggtg
    10201 cagggcctcg tgcagcgggg tcgcaaggag accaacgagg tgctgcgcga cctggagcag
    10261 ctgatgggtg gcagcgacct gcgcaagcgc ggcgttgata ccgcgagtcg agcccgcagg
    10321 caggtcggtg atgccacggc acgtgtccgc cgggcggccg atcccgtgat cgcgcaggcg
    10381 gaccgcgcac ggcgcacggc cggtgtcgga tccaacttcc cgatcgcggg ctacgccgac
    10441 ctgagcgtgg cacaggtgcg cagccgcctc gggagcctca cgcccgccga gctgcgcaag
    10501 gtgcgcgact acgagcgccg caacgcgaac cgcaagacgg ttctgaacga cattgagcag
    10561 cgcctgaacg catagacgct gtgggatgtc cggctgtggt gcagttgtgg ctgctatggc
    10621 gacccaaagt gcgccacgaa cgcccgcacc ccccgccgag ccctgccctc actgctgctg
    10681 ttcagcgaat tccgcgagca tcgccgagaa gtcgagctga gcatcgccgc cggtgagctc
    10741 ggccagtagc tcgtcgccgt tcaggcgttg cccatagcgc cacagcgatc gcagctgctc
    10801 gcccgcttcc ggtgacgacc accaggcttc gccgaagcgc tcacggaaat ggcgacgcag
    10861 gtgcgtctcg agcgcccacg cgcgcagata gcgggcggag tagaagaacg ggtccacatc
    10921 ggagagccag gtctcgcgcg gccagtccac gtgcacggcg tcagagaggc gccgacggta
    10981 cacgtcgggc agcgcgtcga gatcggccgg cgcgtcctgc agctcgagct cgtacgacag
    11041 cttcgcgcag tagcgccgca ccagcaccag cttcgccgcg cgcgcctgct ccaccacctc
    11101 gtccgggctt gccacgccta gcgcgtcgcg cagccagctc ggatcggccg acaggctctg
    11161 gaacagaaac gcgaacgact cggtgacgga gttgtcgccg aggtagcggt cctcgaacgg
    11221 cagcgccgcg tccacgtgcg agtagtgctg cgcgtggccg gcctcgtgga acagcgtctc
    11281 gtagtcctcg cgaccgccgt gtggggcgat caccaggtac acctcgtcgg gcacgcgcgc
    11341 gggtgagcag aacgcccggg gcgccttctt cggacgctcg tccacgtcga gcagcacgcc
    11401 gcgaccgtcc acgctcatcg tgctcatcgt gtggcggtag cgctcgagca ggcgctccgc
    11461 cgggaacgct ccgtcgagcg caggtgcgcg gaagaaggcg ccgatgtcgg cgcgttggaa
    11521 gcggtcgaag ccgtaaccca gctcctcgcg taggcgcggc tccaccagcg gctcgtagct
    11581 cggctcggtg tcgcgcagga acgcctcggt ctggtcggcg agggcggcca ggtcgatgcc
    11641 cgagagcgcc tcggtcatcg cgcgcacgct gcgccagccc agctcgcgaa ccagctcgct
    11701 cgtgcgctcg aagctctcgc ggtggagctg ctgcagctcg cgctcgagca gttcgcgctg
    11761 ggctgcatcg atccgggcgc ggcgctcgcg gtcgggctcg ttcgcctgca cgacgggcgc
    11821 ctggcggacc ggcatgcgct cgccgtcgac ctcgatctcg agcgccgcct cgcggcgcgc
    11881 cagctcggcg tcgaggtgct tcgtctcctg gccgatcagt cccttcgccg cgaagtcgag
    11941 cagctcgcgg ttgccggatt cacgcaggcg ctccacggct tcgcgcgtga ctaggccggc
    12001 atggcgctcg tagatcggct cgatctcgag ctcgtccttc tgccccgaga aatggaggta
    12061 gtactcgcgg tcgagcgcgg agacgaacgc ctccgcctcc tctcgatagg cgctcagatc
    12121 cacgggctgc cagcatagga gaccgtgact cagcagcaga caggggcgcg cccgaagcag
    12181 ggtgacgagc tcgatctgac gatcgagtcg ctcgctcacg gcggcaacgg cgtggcccgc
    12241 accgagggct acgtcgtgtt cgtgaagggc gcggtcccgg gcgatcgcgt ccgtgcgcgc
    12301 gtggagaagc gcaagcgcgc ctacgccgag gcgcgcgccg tcgagctact cgagccctcg
    12361 cccgagcggg tcgaaccgcg cgcgcctcac ccgggcgcgc cgtggcaggt gctcccctat
    12421 gagcgccagc tcgaggagaa gcacagccag gtggaggacg cgctgacgcg gctcgggggc
    12481 ttcagcgacc caccggtcga gccgatcgtg cccgcagtcg agcagtggcg ctaccgcaac
    12541 aagctcgagt actcgttcgg cgaggacgag agcggccggc tcgtgctcgg cttccaccgc
    12601 cccgggcgct ggaacgacat cgacgacgtg actgaggaca tcctcgcctc cgagcgcatc
    12661 gacggcgtgc gcgagcgtgt gaaggagtgg tgccgggacg agggcctgag cgcgtacgac
    12721 aggcgcgaca acaccggctt cctgcgcaac ctcgtggtcc gcgaggggcg tcgcacgggg
    12781 cagctccagg tccgggtcgt cacgagcccc ggcgagttca acgccgacgg gctggcggcg
    12841 gtcctcgagg acgcccacgg cgtgctgtgg acgcagaccg agggcgtcgc cgaaacgacg
    12901 cgcgagggca gcaccaagct gcttcgcggc aagaagacga tcgaggacga gatctgtggc
    12961 cttcgcttcc gcatctcgcc cgaagcgttc ttccagacga acaccgagat ggccgagatc
    13021 ctctacggca ccgcgatgga gctcgccggc ctgtcgggcc gcgagcgtgt gttcgacctc
    13081 ttctcgggga tcggcacgat cgccctcgcg atgtccaccg aggcccgtga ggtcttcggg
    13141 gtcgaggtga tcgagcaggc cgtcgccgac gcgatccaga acgcgcgcct caacggcgtg
    13201 gacaacgcac gcttcttcgc gggcgacgtg cgcctcgcta tgcgcgaact cgtcgaggag
    13261 gcgggccgcc cggacgtgct cgtcgtggat ccgccgcgcg cgggcctctc cgcgaagatc
    13321 gtgcggcgca tcctcggggc cgagccaacc cgcatcgtgt acgtgtcctg caaccccacc
    13381 acgctggcgc caaacgctcg acagatggtg gagggcggct accggctcga cgcggtccgg
    13441 ccggtggaca tgttcccgca gactccgcac atcgagtgtg tggctgcgct gtctcgtgct
    13501 taacaaagcc tgagcagccg ccgataccac gtcgtgtgag ggattcgcgt gcggctgcgc
    13561 gcatcgttga cgtctccgac gtccgctttc gcgtgggcac gatcaagatc ggcgtgggga
    13621 tcacgctggc cgtctgcagc gtgggcatcg cctacgcgat cgccacctgg aaccacgcga
    13681 accgctcgct catcgtcgtg ctgctcgcgc tcgcgtggct cagcgcctgg gtgatcgctc
    13741 tcatggacgc ggagaagatc gtgcgcagtc gctggcgcga gccgttcttc ttcctctggt
    13801 cggccctcga cgtcgcgatc gtcggcgcac tcgaggcggc tgatggcggc gcccgcagcc
    13861 ccaccgtcct gatctatgtc ctgccgctcg cattcgccgc gctctcgtac ccgctgccgt
    13921 ccgtcgtggc gatctctctc ctcgacgtga tcacctacgt cgccgtgagc gcggcggccg
    13981 atccgcccgg cttcacctac atcgcgttcg tggcggcgtc gctggcctgc gcggcggggc
    14041 tctgcgtggc gcaggcgcgc aaccatcacg cccagcgcgc ggagctcggg cgcgtctcgc
    14101 gcaccgaccc gctcaccgag gcgctgaacc gccgcggctt cgaggagcgg ctcgacgcgg
    14161 cgctcgcgga ggcccaccga gagcgcactc cgcttgcgct gctgctgctc gacctcgaca
    14221 acttcaagct cgtcaacgac acgcgggggc atgcggccgg cgacgagctg ctctgctggg
    14281 tcgttgcgac cgtgcgggcc acgctacggc cggtcgacgc tctcgggcgg cttggcggag
    14341 acgagttcgc tgtggtgcta ccgggcgcga accgtgccga ggcattggag gtcgcggaac
    14401 gcgtggagcg gacgctggct ccgcgcgtgc aggccactat cgggctcgcg gccttcccgg
    14461 ccgacggcag cgagcgcgag gagctccacc gccacgccga caatgacctc tacggggcga
    14521 agcacgagcg agccatcgcg cgcgagagcg ccccgcgcga ggtcgcgcag cctgcaaacc
    14581 gcgccgacgt cacggcctac gcgcgcgcca tcgctaagca gctcggcctg gagggcgacg
    14641 aactgtcgcg cgtcctgacg gcggccgagg agctcgaggc gcacctcgcc gccgcggtgc
    14701 aacaaaagcg gatcgcctag ggctcgagcg cgtcttcctc gccgagcggg acctcttctg
    14761 ccgcgtgcac cgagcagagg aacggaccgc cgctgtcgag cgacgcctgg atctcctggt
    14821 cagtgagctt ggccccgcat tcggcgcacg tgcgctcgat caccggctcg acgcctgtgt
    14881 gctcttcgag gtccatcgcg cccggagtct acgcacagga ggggtgctgc gtcagccccc
    14941 cgccggcacc gtcgaatcgc tcgcgacgac gtgctgcacc ggcaaccccg tgacccgctc
    15001 gacccggtgc ggcaggtcca gcttgagcca cttcgacacg tgcacgggca gtgtggagca
    15061 gatcacctcg tcgaacttgt cgaagttcac tgcgtcctcc acggcagcca cgggatccgg
    15121 atcgcctgcc ttgccctcga ccgtcaggcc cgcctggcgt aggcgctcga cggcgttcgc
    15181 cacgtgctgc tctgcctcct cgccgcccga gtgcatgtcg gcggcccagg ccacgccgtg
    15241 tggtgtggcg ggcacgagca gggtgaactt cacgtcccca tcggcggctc tctgcttcag
    15301 cgcctcgaga agctctggcg actccgcggt gcggttggct acgaccagca cattggtcat
    15361 cgatgcctcc tcttccaact cagccgtccc aaacggtacg cccctggggc gggcgctcgc
    15421 aaggagctag tagggaggga ggtccgcgta ccagtcgccg agcgagcgga aggcacgcca
    15481 gaaggagcgc ttcgcgctct cctcgtcgag cgcggagtcg atgttcggca cgtagaggaa
    15541 gtccatcgcc ggcgtgagcc gctgcagatc tccgggccgc gcctccaccg ggcgcgacag
    15601 cggcacgccg agctcgttca gctcgtcgag cgcgtcctcg cccatcgcga cgacgatgcg
    15661 cggctgcacg atcgtgagct cgtcgagcac gcgcgcgcga cactcaggcg ccgagtcctc
    15721 ggtgtcgtgg accgggcact tcacgaagag cgtgccgtag acggcgagcg ggtcgatcgc
    15781 gagccgcttg aagctcttca tcagggcact gccggagcgc ccgtagaaag ccacgccctc
    15841 ctcgatctcc gagggcatgg gcgcgtactt cactaggaag atgtcggctt gcgggtgtcc
    15901 cgatccgagc accggcatca ggtttccgcg cgggcagtat tggcagtcct ggagctcccg
    15961 cgtgagctgg ttgagctcgc ggatcgcgcg ctccaggtac ttctcgcgta tctcgtcgtc
    16021 ggtggccggc actctcaccg tgggtttgga cggagcgtcc ccggctcctc cgcgttgcag
    16081 cggtctttgc ggctctggcg tcaatcttgc ggcgcggatg ccccgcgccc ggctcggcgg
    16141 cgcgactgcg aggggcgcgc cttggtctgc aggaacttga gcgcctggac gtagagcagg
    16201 ctctgcttct tgcgcacgat gtcggcgtcg cgcagcgact cgaggaactc ctcgggcccg
    16261 tcgaagtcgc gcatgcggat cttcactgac ccgagcccct gcaccacgtg gctgtcggag
    16321 cccgccccgg ccacgatccg gtacttggcc gcgaagcgcg ctgcctcctc gttgaacgcc
    16381 gagaacgcga cgcgcggatt gaagacctcg atcgcgtcga tgtcgccgac cacgccgagg
    16441 aggtgctcgt agtccggcac cgagtgcatg cggtcgaacg ggtgcggcac gtacaccagg
    16501 ccgccctggc ggcggatctc ggcgatcgtc tccgcgagcg tcatcccgcg tgggatcttc
    16561 tcctcgatga agagaccgat gacctcgccc tggtcggcgg tcttcacctc ctcgccgacg
    16621 atcaccttca cgcgtccgtt cgtgtgctcg cgcgcctcga gcgccccgga gatctcgttg
    16681 tggtcggtca ccgcgatggc gccgaggccc acgcgcgcgg cggtgtcgag cagcgactcg
    16741 accggggttg cgcagtcgtg cgagtggtcg gtgtgcatgt ggaggtcgac gtcgacgagc
    16801 ggccggctct gcagctgtga gcgcagcgcg gggtcgccgc ggccgtcgtg gcggcggcgc
    16861 accaggcgcc cgtagacggc c
//
LOCUS       DASVXS010000224         5688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1336143, whole genome shotgun sequence.
ACCESSION   DASVXS010000224 DASVXS010000000
VERSION     DASVXS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336143"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..1556)
                     /locus_tag="VF752_12490"
     CDS             complement(450..1556)
                     /locus_tag="VF752_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8856407.1"
                     /translation="MRAAARERLAVASRLGARLMSLQDPAEIARVTARELQQAFGCFL
                     VAILWVDGDGVLVPVAGCGPLVDEMEDPYAWRQHVRSGVMGRVARTGEPALISDTALD
                     PDFIHPDVETQAGSELAVPIRMGGDIWGVLNLENVATGAFDTDDLLFADMVAIQVGSA
                     LQRSSLLEGLEGTFMNTLAMLSDALETKDSYTADHARQVADLTERVGARLGLVGQQLR
                     TLRYGALLHDIGKIGVPTEILNKPGPLTDDEFERVKEHTVIGERMLRHIPFFESVHPL
                     VRSAHERWDGGGYPDGLEGDEIPLGARIICACDAFHAMTSDRPYRAGTSHEQALLELR
                     RHAGTQFDAAVVEALCAEIQAGGFDAQLVEARTA"
     gene            1897..2988
                     /locus_tag="VF752_12495"
     CDS             1897..2988
                     /locus_tag="VF752_12495"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX8856408.1"
                     /translation="MAVNGREAALLVVFCSHEYDHAALLRAVNEQSGGAPLIGCSTAG
                     EIATDGPGDASAVVVAFGGDGFCASTSVSRGASSDLRAAGADVANAVASLNGGHDERV
                     LLLLTDGLAGDQQEVVRGAYGVAGAAIPLVGGCAGDGLEMKSTVQFHGSEVLTDSVVS
                     AALGSDGPLGIGVRHGWRKVGEPMLVTKASYNRVYSLAGEPALDVYLDRLGAPEEARH
                     DPAAFTRFALTHPLGLDRRSGEEHVRFIGEADFEERSIISIAEVPQGGLAWFMEGDSD
                     SVLRATDAACADAVDALGGSAPLGVLAFDCIARRGVLGEEGIKAEVDRVSERVGNAPV
                     AGFYTYGEFARTRGISGFHNQTLVVMAVG"
     gene            2997..5213
                     /locus_tag="VF752_12500"
     CDS             2997..5213
                     /locus_tag="VF752_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8856409.1"
                     /translation="MGRNWSTQQLVEFLAAVSSFGDEGAATRGGAERAAEALEAEVAA
                     VVRDDRVVTSVGFPPGEVPEQELLEVARGTRRDVGVPGLGVCEAVRAPLDESVPGTLI
                     VSRCDGGFSSEELDLLRGMGRVLGLNLGMIRALESLQQRQRLLETLAGIHRMISRRAP
                     LTDVLQAVVEGAAGLLGDSVASVRLVEQDDPSTMTIVAQTGMTDEQLERLRRTPVGEG
                     LGGRAIAEGRVVVTNEYGEMPGALADRGLQAAIAAPVHDSGRTVGALVVGSYDGDRRY
                     GSHEHDTLVAFAEHASLAVSDAKLADALVYQALHDSLTGLPNRSLFMDRLGHCLARST
                     RGMNPVAVMYIDVDGFKSVNDSLGHAAGDELLVTVAGRLDSCVRPGDTVARLGGDEFV
                     VLLEEVDSQEETRRIAERVLEELRKPCTLHGQEVIMTVSVGIAVGREGAAELVRNADV
                     AMYRAKSTGKGRYEVFEPGMRDRIVERVTLERHLRRAIEGDELTLAYQPIVRLDSGEI
                     VSVEALARWTDPELGVVPPNRFIPIAEEAGLILPLGAWVLRQAVRQAAKWRGRLGDAA
                     PTVSVNVSGIELEQADVVARVSETLHEEGLPAASLVVEITETVLMDDSNHVRATLAGL
                     RDLGVQIAVDDFGTGYSSLQFLSRFPIDVLKIPKPFVDDVDAPSGSSALARAIIDLAE
                     NFSLRTVAEGVEQPGQALTLRTLGCTWGQGYHFARPVDSDALGELLYGAGAASPQM"
     gene            complement(5204..5515)
                     /locus_tag="VF752_12505"
     CDS             complement(5204..5515)
                     /locus_tag="VF752_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856410.1"
                     /translation="MTSASSTKPVSPELERARERLAEALDTRDRLRDRFDASIGTSLE
                     LGAYARLRRAGEEVAAWDRWLRWLESEDFLRAPTPEDRLMEGLLEAETPAMPPGVPLY
                     I"
BASE COUNT          831 a         1947 c         2044 g          866 t
ORIGIN      
        1 atgagccgga tgtccgatcg cgcgagcgcc ttccacgccg cgaggttgtg cacgccgggt
       61 agcccaaagg ccacatcgac tccgcacgcc tcgagccctc tgaccaggag gtcgccgcca
      121 gtcacggctg gggttctaac aggacatcga taactgcaca cgacacgccg gtccccggaa
      181 ggcgtgtgca gttattgacg acatcccctc gatgttcctc acgcgggggc aacgagggcg
      241 acgaacggct tgactcgctc gtggtgcact tgtggctgcc ataacgacca caagtgcgcc
      301 acagttgcat agagcctcgc tgtccgcgtc gccgtggctc ccggacgaag ttcgaagact
      361 tttggtggct atagcgccca aaaggcttca atcacgcgtg tggacgccgt gattgctgcc
      421 gtgaaccgtc gcggtgccct gagagcgacc tacgcggttc gcgcctcgac gagctgcgca
      481 tcgaagccgc ccgcctggat ctcggcgcag agcgcctcga ccacggccgc gtcgaattgc
      541 gtgcccgcgt gccggcgcag ctcaagcagc gcctgctcgt gcgaagtgcc cgcgcggtag
      601 gggcgatcgg aggtcatcgc gtggaaggcg tcgcaggcgc agatgatccg cgcgccgagc
      661 gggatctcgt cgccctcgag cccgtcggga tagccgccgc cgtcccagcg ctcgtgggcc
      721 gagcgcacga gcggatggac ggactcgaag aaggggatgt ggcggagcat gcgctcgccg
      781 atcacggtgt gctccttcac ccgctcgaac tcgtcgtcgg tgagcggccc cggcttgttg
      841 aggatctccg tcggcacgcc gatcttgccg atgtcgtgaa gcagcgctcc gtagcgaagc
      901 gtccgcagct gctggcccac caggccgagc cgcgcgccga cacgctccgt cagatccgcg
      961 acctggcgcg cgtgatccgc cgtatacgag tccttcgtct cgagcgcgtc cgagagcatc
     1021 gcgagcgtgt tcatgaaagt tccctcgagt ccttcgagga gggagctccg ctgaagggcg
     1081 ctccccacct gtatggcgac catgtccgcg aagagcaggt cgtccgtgtc gaacgcaccc
     1141 gtcgcgacgt tttcgaggtt caacacgccc cagatgtctc ctcccatacg aatggggacc
     1201 gcaagctccg atccggcctg cgtctccacg tcggggtgga tgaagtcggg atcgagggca
     1261 gtgtccgaga tcagggccgg ctcgcccgtg cgagccacgc ggcccatcac gccgcttcgg
     1321 acgtgctggc gccatgcgta cgggtcttcc atctcgtcga cgagcggtcc gcagcccgca
     1381 accggaacga gcacgccgtc gccgtcgacc cagaggatcg cgacgagaaa gcagccgaac
     1441 gcctgctgga gttctcgggc ggtgactcgc gcgatctcgg ccgggtcctg cagcgacatc
     1501 agtcgcgcgc ccagccgact cgcgaccgcg agccgctccc ttgcggccgc cctcacgctc
     1561 gacgtaggag aggatcaggc gcgaggtgat cccgctcgtc tcgcgggcct ccgccgcctc
     1621 cccgggcgca ggcatggcaa gggccgctgt gggcgcggtt cccatatgca ggttttcggc
     1681 gcctgcacgg tcccgcttga ggcgcgacag cggcggattt accccaaacg cggtacggct
     1741 gtcagcgaac cgctaaggac caatccggta cctgccgaac acacgggggg aacgagcttc
     1801 ggaggacgct ggatgaatgt tggacgcgat cgctggatga atgttggccg ctctgccgag
     1861 agcgatgctg cccgcgcagg gagcgaggcg gccgcagtgg ccgtgaacgg ccgcgaggcc
     1921 gcgctgctcg tggtcttctg ctcgcacgag tacgaccacg ccgctctgct tcgggcagtg
     1981 aacgagcagt ccggcggcgc gccgctgatc gggtgctcca cggcgggtga gattgcaacc
     2041 gacggccccg gagacgcgag cgcggtggtc gtggcgttcg gcggcgacgg attctgcgcg
     2101 tcgacctcgg tgagccgcgg cgcttcctcc gacctacgcg ctgccggcgc cgacgtcgcg
     2161 aacgccgtcg cgagcctcaa cggcgggcac gacgagcgcg tgctgctcct gctcacggac
     2221 ggcctcgccg gcgaccagca ggaagtcgtg cgcggcgcct acggcgtagc gggcgcggca
     2281 attccgctcg tcggcggctg cgcgggcgac ggcctcgaga tgaagtcgac cgtgcagttc
     2341 cacggttccg aggtgctcac cgattcggtc gtgagcgcgg ccctgggctc ggacgggccg
     2401 ctcggcatcg gtgtccgtca cggctggcgc aaggtgggcg agccgatgct cgtgaccaag
     2461 gcgtcgtaca accgcgtgta cagcctcgcg ggcgagccgg cgctcgacgt ctacctcgac
     2521 cggctcggcg cgcccgagga ggcccgccac gacccggcag ccttcacccg cttcgcgctc
     2581 acgcacccgc tcggcctcga tcggcgcagc ggcgaggagc acgtgcgctt catcggcgag
     2641 gccgacttcg aggagcgctc gatcatctcc atcgccgagg tgccccaggg tgggctcgcg
     2701 tggttcatgg agggcgactc ggattccgtg ctgcgcgcaa ccgacgccgc ctgcgccgac
     2761 gccgtcgacg cgctcggcgg cagcgcgccg ctcggcgtgc tcgccttcga ctgcatcgcc
     2821 cgccgcggtg tacttggtga ggagggcatc aaggccgagg tggaccgcgt ttccgagcga
     2881 gtcggcaatg cgccggtcgc gggcttctac acgtacggcg agttcgcgcg cacacgcgga
     2941 atcagtggct tccacaacca gacgctcgtc gtcatggcgg tgggctgagg atgccgatgg
     3001 gccggaactg gtccacccag cagcttgtcg agttcctcgc ggccgtctcg tcattcggcg
     3061 acgagggcgc ggccacgcgc ggtggcgctg agcgcgccgc cgaggcgctc gaggccgagg
     3121 tggccgcggt tgttcgcgac gaccgcgtgg tgacctcggt cgggttcccg cccggcgagg
     3181 tgcccgagca ggagctgctc gaggtggccc gcggcacgcg gcgcgacgtc ggcgttcccg
     3241 gtctcggcgt gtgcgaggcg gtgagggcgc cgctcgacga gtctgtgccc ggcacgctga
     3301 tcgtgtcgcg gtgcgacggt ggcttcagtt ccgaggagct ggacctgctg cgcgggatgg
     3361 gccgtgtgct cggcctcaac ctcgggatga tccgcgcgct cgagtcgcta caacagcgcc
     3421 agcgtctgct cgagacgctc gcgggcatcc accggatgat ctcgcgtcgg gcgccgctca
     3481 ctgacgtgct ccaagcggtc gtggaaggcg cggcggggct gctcggcgac tcggtcgcga
     3541 gcgtgcggct cgtggagcag gacgatccct cgaccatgac gatcgtcgcc cagaccggca
     3601 tgacggacga gcagctcgag cggctgcggc ggacgccggt gggcgagggg ctcggcggtc
     3661 gggcgatcgc cgagggccgc gtggtggtga ccaacgaata cggcgagatg ccgggcgcgc
     3721 tcgccgaccg cggcctgcag gccgccatcg ccgcgcccgt gcacgacagc ggcaggaccg
     3781 tcggcgcgct cgtcgtcggc tcctacgacg gcgatcgccg ttacggctcc cacgagcacg
     3841 acaccctcgt ggcgttcgcg gagcacgcga gtctcgccgt gagcgacgcc aagctcgccg
     3901 acgcgctcgt gtaccaggcg ctgcacgact cgctcaccgg cctgcccaac cgctcgctgt
     3961 tcatggaccg cctcggccac tgcctggcgc gcagcacgcg cgggatgaat cccgtggctg
     4021 tgatgtacat cgacgtcgac ggcttcaagt cagtgaacga cagcctcggt catgccgcgg
     4081 gcgacgagct gctcgtgacg gtcgccggca ggcttgactc gtgtgtgcgg ccgggcgaca
     4141 ccgttgcgcg cctcggcggc gacgagttcg tggtgctgct cgaggaggtc gactcgcagg
     4201 aggaaacgcg ccggatcgct gagcgcgtgc tcgaggagct gcggaagccc tgcacgctgc
     4261 acggccagga ggtgatcatg accgtgagcg tcggcatcgc ggtcggccga gagggcgccg
     4321 cggagctggt ccggaacgcc gacgtggcga tgtaccgcgc gaagtccacc ggcaagggcc
     4381 gctatgaggt gttcgagccc ggcatgcgcg accgcatcgt ggagcgcgtc acgctcgagc
     4441 gccatctccg ccgcgcgatc gagggcgacg agctgacgct cgcctaccag ccgatcgtgc
     4501 gcctcgacag cggcgagatc gtgagcgtgg aggcgctagc gcgctggacc gacccggagc
     4561 tcggcgtggt cccgccgaac cgcttcatcc cgatcgccga ggaggccggc ctgatcctgc
     4621 cgctcggcgc ctgggtcctg cggcaggccg tgcgccaggc cgcgaagtgg cgtgggcggc
     4681 tcggcgacgc cgcgccgacc gtgagtgtga acgtctcggg catcgagctc gagcaggccg
     4741 acgtcgtcgc gcgcgtgagc gagacgctcc acgaggaggg gctcccggca gcctcgctcg
     4801 tcgtcgagat caccgagacc gtgctgatgg acgactcgaa ccacgtccgc gccacgctcg
     4861 ccgggctgcg cgacctgggc gtgcagatcg cggtcgacga cttcggcacc ggctactcgt
     4921 cgctgcagtt cctcagccgc ttcccgatcg acgtgctgaa gatcccgaag ccgttcgtgg
     4981 acgacgtgga cgcgccgagc ggctcatccg cgctcgcgcg cgcgatcatc gacctggcgg
     5041 agaacttcag cttgcgcacc gtggccgagg gcgtcgagca gccgggccag gcgctgacgc
     5101 tccgcaccct cggctgcacg tggggtcagg ggtaccactt tgcgcgcccc gtggactcgg
     5161 acgcgctcgg tgagctgctc tacggcgccg gcgccgcctc gcctcagatg tagagcggga
     5221 cgcccggcgg catcgcgggg gtctcggcct cgagcagtcc ctccatcagc cggtcttccg
     5281 gtgtgggcgc gcgcaggaag tcctcggact cgagccagcg cagccagcga tcccatgccg
     5341 cgacctcctc gccggcccgc cgcaggcgtg cgtaggcgcc gagctccaac gaggttccga
     5401 tcgacgcgtc gaatcggtcg cggagacggt cgcgtgtgtc gagtgcttcc gccagtcgct
     5461 ctcgcgcgcg ctcgagctcg ggagacacgg gcttggtcga tgaggccgag gtcatcgctt
     5521 ctccttccgc cacgaggcgg ctgaacgggg acttggaagg gaaggccggc gcacgcccgg
     5581 gccgcggtgg ggagggctct gcgacccgag cgctggagac agccggcgat ccataggttc
     5641 gatggagtgt ctaaagcagc cttaagaccg tggttgcaat tcggtaaa
//
LOCUS       DASVXS010000225        10294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1234695, whole genome shotgun sequence.
ACCESSION   DASVXS010000225 DASVXS010000000
VERSION     DASVXS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234695"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VF752_12510"
     CDS             <1..571
                     /locus_tag="VF752_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX8856411.1"
                     /translation="GPGPGPGTGPILGGARPDDPWYRRINWPEPKYIALIVAGVLILG
                     GGAAYGVKQLTDDNGSSNAPVTSGNTGQTGGGKKPGKRAGTQVNPADIRVSVLNGTTV
                     TGQASKLADQLENKGYQRGTVANASGDQGQRAESVVEYSDGNVAAARAVGRELGITQF
                     EKADPTTQQLAGDAQVIVILGADKAAATG"
     gene            592..2373
                     /locus_tag="VF752_12515"
     CDS             592..2373
                     /locus_tag="VF752_12515"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX8856412.1"
                     /translation="MPSSILVRAVFGLLVLSTVGAFFVTQRLKRSTPIVERVFYKPYL
                     SPNGDRRKDTASLRFDLPKRERVTAAMVNDAGDPVRTLLDDRRRSRGTVHMTWDGRDD
                     DGAIVPDGVYHLRVTLPTEGRAVTSPRDLIVDTKPPRPAIVAVTPPTILPGDTAKRGD
                     ARIRFRGPSNPKPLFRVYRTDVAGPPRLVRSFSGRRGRQTGVWDGRLQDGRLAPDGIY
                     SISVTVQDKAGNAGSAPATLPPSRSSVTRRSGVSARYLTLMGPLEPVNAGSVVKLTIG
                     PKARRARWNLTRIGPGRPVARGESHGTKVAIRVPGDARTGLYLVRVQAAGHRAIEPLV
                     VRGRGRGPVLVVLPSITWQGLNPVDDDRDGFADTLSDSLSVQAARPFAHGRLPAGLDS
                     TEIPLLRFLDRSKLPYDVTTDLALARGQGPGIDGHGGIIFAGSERWLTEKLDLALRSF
                     VEKGGKVASFGTDAFRRRVDVGPTTLSSPSAPEAVNVFGEQVGETRIEPAPMVVNRDA
                     PAIQLFAHTDGFIGQFQDFEQSRKLVGGAIVQSAAGRDPKQPALVAYKLGDGLVIRMG
                     TPEWATSINGDIEVAQVTRRTWQLLSR"
     gene            2352..3953
                     /locus_tag="VF752_12520"
     CDS             2352..3953
                     /locus_tag="VF752_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8856413.1"
                     /translation="MATPLAVGVAAKEAGMIVAALAVAGAFFVPSARGRAVCVLVALV
                     LTPVLLVGELWDSSQFVSIRDHPAPAVGAAVLGVAVMGALAAVFLRRPEAFPLLAVFT
                     LPFRIPVEAGGQTANLLVPLYAVVGGAAIAYAWERLRGSNGGWNGQARRPFCPDERPP
                     GTLEAVLIGAVVLYTLQAAYSTDFETALKNVVFFYVPFALLFRFLTSLEWNERVVRRA
                     FGVALLLALVFVSIGFWEYATRHLLWNPKVIASNEFESYFRVNSLFFDPSIYGRFLAL
                     VMIGLATVLLWARRTRNVWVVTGLLAFLWAGLVLTFSQSSFVALLVGLAVLAALRWRA
                     RPVVATVAAAAIVAAIVVVAVPSAVNLDVGSSRSVSQASSGRFDLIGGGLRMFADRPV
                     WGFGSGSFSKEFRQREKVSSQRAASASHTIPLTYAAEQGVVGFAAYVAVLWAAFRLLF
                     RSLSGPLRGREPPGDKALARAAIAAAFAALVVHTLLYASFLEDPITWTLLGAGLALQR
                     RPRRVRTGAAAATASEQVPAEPAAG"
     gene            complement(3950..4852)
                     /locus_tag="VF752_12525"
     CDS             complement(3950..4852)
                     /locus_tag="VF752_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX8856414.1"
                     /translation="MTGSALILLREGLEGALIVAIVLAYLRKLDRRDGFRHVWAGTAL
                     AVGISVLVGAILFVTIGELQGDARKTVFAIIMLFAACVLTWMIFWMGRQARMLKGELQ
                     HKVDTALIAGAGGALVAVVFFAVLREGIETALFFVAAAGQSSTLDSLVGGSLGLAGAL
                     VLAYAFYRGARWLDLRLFFAVSGGLVLLFAAGLLARSVAQIQEVGALTTYWYPVFDAS
                     SVGLVSTDHFFGELLRGLFGWDPAPSIEELGVWLGYVVVVGTLYARSLRGMRKPRPTA
                     APAAAAAPQRQQPEIARAPEAADA"
     gene            complement(4913..6526)
                     /locus_tag="VF752_12530"
     CDS             complement(4913..6526)
                     /locus_tag="VF752_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8856415.1"
                     /translation="MRNWSRRRRWLTIGGGVLLVLAVGLLVAYLTVGQRPGDITNPDV
                     EFTTPPKQPEQKQEQARRKGKPFAWPVYGFDAQRTHWLDPPNHLARPPFRRAWTLGGS
                     HLIEFQPVLAKNTLYFQKNNGEAYAVNALTGKVRWRKHIARLAASSPAVSGKRAFFTT
                     LSGRIVALNTKNGRILWARNLGSRSESSPLVIGDKLYFGSESGEVYGMRQRDGRVLWT
                     YQAGGDVTAGPAYSNGLLYFGDYGGNMQAVRRTNGHLVWKTHTSGLPLGRSGGFYATP
                     AVAFGRVYAGNLDRKVYSFSARSGKIAWSHTTGAYVYAATAVADADGSPPSVYVGSYD
                     GHFYALDARSGRQRWVYNANGHISGAATVVGNIAYFSNLSAHSTTGLDVRSGRPVFKI
                     GRGAYNPVISDGQRLWLTGYSSVYMLESKAFLRAQARKRAHRHLTPAQKRKAAKRRQA
                     ARKRAARKRELQAKRRAAARKRAQRRQICTRGPNGTVVCRYYGRKLSCRVHGRHLHCR
                     GVQHRLVCRRSHGRLTCHRDTTHRRRSNR"
     gene            complement(6561..7496)
                     /locus_tag="VF752_12535"
     CDS             complement(6561..7496)
                     /locus_tag="VF752_12535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8856416.1"
                     /translation="MLISYCVVNTNGRDLLLAGLEAIERTAPAGVEREVIVLDNASDD
                     GSADAVRALGRDYRLIELSERTGKAENDSTLMRESRGRYSLLLNEDAELQPGAVEALL
                     EALEADPRAAAAGAQLLDSDGTPVPCAWRLPGLGTALAQALFLHKQLVTQSGGSRTRE
                     VGWVQSSAMLVRRDAAEQVGWMDPDFFVYSDETDFAKRLHDAGWRLLHVPAARAIHHD
                     QLASDVEGQRRRIVEFHRNRDLYMRKHHSMPVVLATRLLYALPYLVRAAAALALPRHD
                     PRRYITHARQALQPGRGEGIREAAEERNRCLRKAA"
     gene            7516..8610
                     /locus_tag="VF752_12540"
     CDS             7516..8610
                     /locus_tag="VF752_12540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8856417.1"
                     /translation="MRVQIVDPPAYTPPYDHSLAAALARAGADVELVTSHFRHGPLPE
                     VGGGYRVSERFYRHGDSRLAKLAEHVPGMLSYRGEAQHADVVHYEWLPLEPIDALLLP
                     PKRPRVFTAHNVVAHDAPRGWESARRRLLRRMDAVVVHSEFGARQMSERLGVPPGRVR
                     VVHHGVFDYLTRLPHEEPLFEELRDVEGPVVLFFGLLRPYKGVDVLVEAFREVEGAEL
                     WVVGAPRMPLEPLEHQARDSRSPVRFVPRFVTDPEIPALFRRADLVVLPYREIDQSGV
                     LYTALAFGKPIVASAAGGFVEVAEQHGAARLVPPGDPAALAAAIQQLVGDPAERNRLA
                     QAASAAAAGPYSWDSAARGHLELYRELTGA"
     gene            8607..9731
                     /locus_tag="VF752_12545"
     CDS             8607..9731
                     /locus_tag="VF752_12545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856418.1"
                     /translation="MTVVEILFWLSVAGLVHTHVTYGLSLALLERLRGRRGEAKGAGE
                     LPRVSLIVPAHDEEPVIWDKVRNALALDYPRDRLELIVASDGSTDATVAQARAAGADV
                     VLDLPRAGKVAAQDAAAERARGEILAFADANSTWARDALRRLVEPFADERVGYVCGQV
                     RFSRDEGGTNEEGIYWRYEMAVRGLESRAAGITAGNGAIYAVRRSAYWLDPLRGDLSF
                     PFKLAKRGLRSVYQPQALAQEKMVPTIEGEFRRKRRMMSRAWGTVLEGGMLSPLRYPP
                     LYAYEIFSHRVLRYASPFLHVVAFGTNIALLGNGWVYVLTFAAQCALLLAAALAPFVR
                     ARPFAIAYYYVTITASIAAGLFDWLRRGMPLTWEKPEGTR"
     gene            9728..10291
                     /locus_tag="VF752_12550"
     CDS             9728..10291
                     /locus_tag="VF752_12550"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8856419.1"
                     /translation="MITRAIDIVVAGVALVFTSPIIAAAMIAIRLESPGSPIYKQRRV
                     GLDGEPFEIWKLRTMYLGSDRGPDRAIPKDDPSITRVGAFLRRTSLDELPNLINVLRG
                     EMAIVGPRPTIQVQVDQYTPRQRRRLEVKPGLTGWAQVNGRVGLPWPERIELDVWYVD
                     HRSLRLDLQILAKTVRLLVTGHGLYTS"
BASE COUNT         1447 a         3572 c         3684 g         1591 t
ORIGIN      
        1 cggtcccggg cccgggccgg gcaccggccc gatcctcggc ggcgcgcggc cggatgatcc
       61 ctggtaccgg cgcatcaatt ggccagagcc caagtacatc gcgctgatcg tcgccggcgt
      121 gctgatcttg ggcggtggcg ccgcatacgg cgtgaaacag ctcacggacg acaacggcag
      181 ctcgaacgct cccgtcacct cgggcaacac cggtcagacc ggcggcggca agaaacccgg
      241 caagcgcgcc ggcacccagg tcaatcccgc cgatattcgc gtgtcggtgc tgaacgggac
      301 cacggtcacc ggccaggcca gcaagcttgc ggatcagctc gagaacaagg gctaccagcg
      361 cggcacggtc gcaaacgcga gcggtgatca gggccagcgc gcggaatccg tggtcgagta
      421 ctcggatggc aacgtggcgg cggctcgcgc ggtggggcgc gagctcggga tcacgcagtt
      481 cgagaaggcc gaccccacca cccagcagct tgccggagac gcccaggtga tcgtgattct
      541 gggcgccgac aaggctgccg ccacaggctg agcgccatta ccgttacgcg cgtgccgagc
      601 agcatcctgg tccgcgccgt attcggcctg ctcgtgctgt ccacggtcgg cgcgttcttc
      661 gtcacgcagc gcctgaagcg ctccaccccg atcgtggaga gggtcttcta caagccctat
      721 ctgtccccga acggcgacag gcgcaaggac acagcctcgc tgcgcttcga cctgcccaag
      781 cgcgagcgcg tgacggccgc aatggtgaac gacgccggcg acccggtgcg cacgctgctc
      841 gacgaccgcc gccgctcgcg cgggaccgtg cacatgacct gggacggccg cgacgatgac
      901 ggcgcgatcg tgcccgacgg tgtgtaccac ctgcgggtga ccctgccgac cgagggccgg
      961 gccgtcacct cgccgcgtga cctgatcgtg gacacgaagc cgccccggcc ggcgatcgtc
     1021 gcggtcacgc cgcccacgat cctgccgggc gacaccgcca agcgcggcga tgcgcgcatc
     1081 cgcttccgcg gtccgtcgaa cccgaagccg ctgttccggg tttatcggac cgatgtcgcc
     1141 ggaccgcccc ggctcgtccg gagcttctcg gggcgtcgcg gccgccagac aggcgtctgg
     1201 gacgggcgcc tccaggacgg acgactcgcg cccgacggca tctactcgat cagcgtgact
     1261 gtgcaggaca aagcgggcaa cgccggcagc gcgcccgcga cgctgccgcc gtcgcgcagc
     1321 agcgtgacgc ggcgctcagg tgtgtcggcg cgatacctca ccctgatggg cccgctcgag
     1381 ccggtgaacg ccggaagcgt cgtgaagctc acgatcggcc cgaaggcgcg ccgtgcgcgc
     1441 tggaacctca cgcgaatcgg ccccgggcgt ccggtcgcgc ggggagagtc gcacggcacg
     1501 aaggtggcga tccgtgtgcc cggcgacgcg cgcaccggtc tctacctcgt acgcgtgcag
     1561 gcggcgggtc atcgcgccat cgagccgctc gtggttcgcg gccgcggacg gggaccggtg
     1621 ctcgtggtgc ttccgtcgat cacctggcag ggactgaacc cggtcgacga cgaccgcgac
     1681 ggcttcgccg acacgctgtc ggattcgctc agcgtgcagg cggcgcgccc gttcgcgcac
     1741 gggcgcctgc cggccggcct ggattcgacc gagataccgt tgctccgctt cctcgaccgc
     1801 tcgaaactgc catatgacgt gaccaccgac ctcgcgctcg cacgcggcca gggaccgggc
     1861 atcgacgggc acggcggcat catctttgcc gggagtgagc gctggctcac cgagaagctc
     1921 gaccttgcgc tccgcagctt cgtggagaag ggcgggaagg tggcgtcgtt cggcaccgac
     1981 gcgttccgcc gccgcgtcga cgtcgggccc acaacgctga gctcgccgag cgcgccggag
     2041 gccgtcaacg ttttcggaga gcaggttggc gagacgcgca tcgagccggc gccgatggtg
     2101 gtcaaccgcg acgcaccggc aattcaactc ttcgcccaca ccgacggttt catcggccag
     2161 ttccaggact tcgagcagtc ccggaagctg gtaggcggag cgatagtgca gtctgcagcg
     2221 ggccgcgatc cgaagcagcc cgcactggtg gcgtacaagc tcggcgacgg actcgtgatc
     2281 cgtatgggca cgcccgaatg ggcgaccagc atcaacggcg acatcgaggt ggcgcaggtc
     2341 acccgaagga catggcaact cctctcgcgg taggcgtcgc cgccaaggag gcgggcatga
     2401 tcgtggcggc gctcgcggtc gccggagcgt tcttcgttcc gtccgcgcgt ggccgcgcag
     2461 tttgcgtgct ggtcgcgctg gtcctcacgc cggtgctgct ggtcggcgag ctgtgggaca
     2521 gcagccagtt cgtgagcatc cgcgatcacc cggctcccgc cgtgggtgcc gcggtgctcg
     2581 gtgtcgcggt gatgggcgcg ctcgccgcgg tcttcctccg caggcccgag gccttcccgc
     2641 tgctggccgt gttcacgctg ccgttccgga tcccggtgga agcgggcggc cagacggcga
     2701 acctgctcgt gccgctctac gcggtcgtgg gcggcgcggc cattgcctac gcctgggagc
     2761 ggctgcgcgg ctcgaacggt ggctggaacg ggcaggcgcg gcgcccgttc tgcccggacg
     2821 agcggccgcc gggcacgctc gaggcggtgc tgatcggtgc ggtcgtgctc tacacgctgc
     2881 aggcggctta ttccacggac ttcgagacgg cgctgaagaa cgtggtcttc ttctacgtcc
     2941 cgttcgcgct gctgttccgc ttcctcacgt cgctcgagtg gaacgagcgc gtggtgaggc
     3001 gcgctttcgg ggtggcgctc ctgctcgcgc tggtgttcgt ctcgatcggc ttctgggagt
     3061 acgccacgcg acacctgctg tggaacccga aggtgatcgc gtcgaacgag ttcgagtcct
     3121 acttccgggt caactcgctg ttcttcgacc cgagcatcta cggccgcttc ctcgcgctcg
     3181 tgatgatcgg cctggcgacg gtcctgctgt gggcgcggcg gacacgcaac gtctgggtcg
     3241 tcaccggttt gctcgcgttc ctgtgggcgg gtctggtgct cacgttctcg cagtcgagct
     3301 tcgtggcgct gctggtgggc ctcgccgtgc tcgccgcgct gcgctggcgt gcccggccgg
     3361 tggtggcgac ggtggcagcc gccgcgatcg tcgcggcgat cgtggtcgtg gccgtgccat
     3421 cggccgtgaa cctggacgtc ggctcctcac gctcggtgag ccaggcctcg agcggccgct
     3481 tcgacctgat aggcggcggc ctgcgcatgt tcgccgaccg gccggtgtgg ggcttcggct
     3541 cgggctcgtt ctcgaaggag ttccggcagc gcgagaaggt gagctcgcag cgcgccgcct
     3601 cggcctcgca cacgatcccg ctcacgtacg ccgcggagca gggggtcgtt gggttcgccg
     3661 cctacgtcgc cgtgctgtgg gcggcgttca ggctgctctt caggagtctc tcaggccccc
     3721 tgcgcggacg ggagcctccg ggcgacaagg cgctcgcgcg cgcggcgatc gccgcggcgt
     3781 tcgcggcgct cgtggtgcac acgctgctgt acgcgtcctt cctggaggac cccatcacgt
     3841 ggacgctgct aggagccgga ctggcgctgc agaggcgacc gcgcagggtg cggacgggcg
     3901 cagcggccgc aaccgcgagc gaacaggtac ccgccgagcc ggcggcgggc taagcgtccg
     3961 cggcttcggg cgcccgggcg atctcgggtt gctgccgctg gggcgcggca gctgccgcgg
     4021 gggcggctgt cgggcggggc ttcctcatgc cacgcagcga ccgcgcgtag agggtgccga
     4081 ccacgaccac gtagccgagc cagacgccga gctcctcgat cgagggcgcc gggtcccagc
     4141 cgaacaggcc acgcaggagc tcaccgaaga aatggtcggt ggagacgagg ccgaccgagc
     4201 tcgcgtcgaa caccgggtac cagtaggtgg tgagcgcgcc gacctcttgg atctgcgcga
     4261 cggagcgtgc cagtaggccg gcggcgaaca gcagcaccag gccgccgctc acggcgaaga
     4321 acaggcgcag gtcgagccag cgggcgccgc ggtagaaggc gtaggcgagc acgagcgcgc
     4381 cggccaggcc gagcgacccg cccacgagcg agtcgagcgt ggagctctgg ccggccgcgg
     4441 ccacgaagaa gagggccgtc tcgatgcctt cgcgcagcac cgcgaagaac accacggcca
     4501 cgagcgcccc accggcgccg gcgatcagcg cggtatcgac cttgtgctgc agctcgccct
     4561 tgagcatgcg cgcctgccgg cccatccaga agatcatcca ggtgagcacg cacgcagcga
     4621 acagcatgat gatcgcgaac acggtcttgc gcgcgtcgcc ctgaagctct ccgatcgtga
     4681 cgaacaggat cgcgccgacg aggacggaga tgccgaccgc gagcgcggtc cccgcccaca
     4741 catggcggaa cccgtcgcgg cggtcgagct tgcgaagata cgcgagcacg atcgcgacga
     4801 tcagagcgcc ctcgaggccc tcccgcagca ggatcagcgc cgaaccggtc accgttaggg
     4861 aaccctaaca gttttcggcc cgaaaaccca tccgccaggt ctgatatcgg actcagcggt
     4921 tactgcggcg gcggtgtgtc gtgtctctgt ggcaggtcag ccgaccgtgg ctgcgccggc
     4981 agaccaggcg atgctggacg ccgcggcagt ggagatgccg gccgtgcacg cggcagctga
     5041 gcttccggcc gtagtaccgg cacacgacgg tcccgttggg gccacgggtg cagatctgcc
     5101 ggcgctgcgc ccgcttgcgc gcggccgcac gccgcttcgc ctgcagctcg cgcttgcgcg
     5161 cggcccgctt gcgggcggcc tgccggcgct tggccgcctt gcgcttctgg gcgggcgtga
     5221 gatggcgatg ggcgcgcttg cgcgcctgcg cgcgcaggaa ggccttcgac tcgagcatgt
     5281 agacgctcga gtagccggtg agccagaggc gctgcccgtc ggagatcacc gggttgtacg
     5341 cgccccggcc gatcttgaag accggcctgc cgctgcggac gtcgagcccg gtggtggagt
     5401 gcgccgagag gttcgagaag taggcgatgt tgcccaccac cgtggctgcg ccagagatgt
     5461 ggccgttcgc gttgtacacc cagcgctgcc ggccgctccg cgcgtcgagc gcgtagaaat
     5521 ggccgtcgta agaccccacg tagacgctcg gcgggctccc gtcggcatct gccacggcgg
     5581 tcgccgcgta cacgtaggca cccgtggtgt ggctccaggc gatcttccct gaacgggcgg
     5641 agaaggagta gaccttgcgg tcgaggttgc cggcgtagac gcggccgaac gcgacggcgg
     5701 gcgtggcgta gaagccgcct gagcggccga gcggaaggcc ggaggtgtgc gtcttccaga
     5761 ccaggtggcc gttggtgcgg cggaccgcct gcatgttgcc gccatagtcg ccgaagtaga
     5821 gcaacccgtt cgagtaggcc ggcccggcgg tcacgtcgcc gcccgcctgg taggtccaga
     5881 gcacgcggcc gtctcgctgc cgcatgccgt acacctcgcc gctttcagag ccgaagtaga
     5941 gcttgtcgcc gatcaccagc ggcgaggact cgctgcggct gccgaggttg cgcgcccaga
     6001 ggatgcggcc gttcttcgtg ttcagtgcga cgattcgccc cgacagcgtt gtgaagaacg
     6061 cacgcttgcc ggagaccgcg ggcgacgagg cggcgagccg ggcgatgtgc ttgcgccagc
     6121 gcaccttccc ggtgagggca ttcacggcgt acgcctcgcc gttgttcttc tggaagtaga
     6181 gcgtgttctt tgccagcacc ggctggaact cgatcaggtg gcttccgccg agcgtccacg
     6241 cgcgccggaa tggcggccgc gccaggtggt tcggcggatc gagccagtgc gtgcgttgcg
     6301 catcgaagcc atagaccggc caagcgaacg gcttgccctt tcttctggcc tgctcctgct
     6361 tctgctcggg ctgcttcggc ggcgtggtga actcgacgtc ggggttggtg atatcgccag
     6421 gccgctggcc gacggtgagg taggccacga gaaggcccac cgccaatacc agcagcacgc
     6481 ccccgccgat cgtgagccag cgccgccgcc gactccagtt gcgcacgcgg caatggtagg
     6541 aaagccacct gaaggcatcc tcaagccgct ttgcgcaggc accggttgcg ctcctccgcg
     6601 gcctcccgaa taccttcgcc gcgcccgggc tggagcgctt ggcgggcgtg cgtgatgtag
     6661 cgccgcggat catgccgcgg cagtgccagc gctgccgccg cgcgcacgag gtagggaagc
     6721 gcgtagagca ggcgggtggc gagcacaacc ggcatcgagt ggtgcttgcg catgtacagg
     6781 tcgcggttgc ggtggaactc cacgatccgc ctgcgctgac cctcgacgtc gctcgcgagc
     6841 tggtcgtggt ggatggctcg cgcggccggc acgtgcagga gccgccagcc ggcgtcgtgc
     6901 agccgcttgg cgaaatcggt ctcgtcggag tagacgaaga agtcggggtc catccagccc
     6961 acctgctccg ccgcgtcgcg gcgcacgagc atcgcgctcg actgcaccca gccgacctcg
     7021 cgggtgcgag agccgccgct ctgggtgacg agctgtttgt gtaggaacag cgcctgggcg
     7081 agcgcggtcc cgagacccgg aaggcgccac gcgcacggca ctggggtccc gtccgagtcg
     7141 agcagctgcg ctcccgccgc ggccgcccgc gggtccgcct cgagcgcctc gagcagcgcc
     7201 tcgacggcgc ccggctgcag ctcggcgtcc tcgttcagca acagcgagta gcgcccgcgc
     7261 gactcgcgca tgagcgtcga gtcgttctcg gccttgccgg tacgctcgct gagctcgatc
     7321 agccggtagt cgcgcccgag cgcgcgcacc gcgtccgcgg agccgtcgtc ggaggcgttg
     7381 tcgagcacga tcacctcgcg ctcgacgccc gccggcgcag tgcgctcgat cgcctcgaga
     7441 ccggcgagga gcaagtccct gccgttcgtg ttcacgacgc aatagctgat cagcactgtt
     7501 gaatcatcgc ctgcgatgcg cgttcagatc gtcgaccccc cggcatacac gccgccctac
     7561 gaccactcgc tggccgccgc ccttgcgcgc gcgggcgccg acgtcgagct ggtcacgagc
     7621 catttccggc atggaccgct gcccgaggtc ggcggcggat accgcgtgtc cgagcgtttc
     7681 taccgccacg gcgactcccg cctcgcgaag ctcgccgagc acgtgcccgg catgctgtcc
     7741 tatcgcggcg aggcacaaca tgccgacgtg gtccactacg agtggctccc gctcgagccc
     7801 atcgacgcgc tgctgttgcc gccgaagcgg ccgcgcgtgt tcaccgcgca caacgttgtc
     7861 gctcatgacg caccgcgcgg ctgggagagc gcccgtcgcc gcctgctgcg gcgtatggac
     7921 gcggtggtgg tgcattccga gttcggcgcc cgccagatga gcgaacgact cggcgtgccg
     7981 cccgggcgtg tgcgcgtcgt gcatcacggc gtgttcgact acctcacgcg cctgccgcac
     8041 gaggagccgc tgttcgagga gctgcgcgat gtcgagggac ctgtcgttct cttcttcggg
     8101 ctgcttcgcc cgtacaaggg cgtcgacgtt ctcgtcgagg cgtttcgcga ggtcgagggc
     8161 gctgagctct gggtggtcgg cgcgccgcgc atgccgctcg agccgctcga gcatcaggcg
     8221 cgcgactcgc gctcgccggt tcgcttcgtg ccgcgcttcg tcaccgaccc tgagattccg
     8281 gcgctgttcc ggcgggcgga cctcgtcgtc ctgccgtacc gtgagatcga ccagtccggc
     8341 gtgctgtata cggcgcttgc attcggcaag ccgatcgtcg cctcggcggc gggcgggttc
     8401 gtggaggtgg ccgagcagca cggcgcggcg cggctcgtgc cgccgggcga tccggccgcc
     8461 ctagccgcgg cgatccagca gctcgtgggc gaccctgccg agcgcaatcg gcttgcccaa
     8521 gcggcgtccg ccgcggcggc agggccttac tcgtgggact cggcggcgcg cggccacctc
     8581 gagctctacc gggagctgac gggcgcgtga ccgtcgtcga gatcctcttc tggctctcgg
     8641 tcgccgggct cgtgcacacg cacgtgacct acgggttgtc gctcgcgctg ctcgagcgcc
     8701 tgcgcggtcg ccgcggcgag gcgaagggcg caggcgagct gccgcgcgtc tcactgatcg
     8761 tgcccgcgca cgacgaggag ccggtgatct gggacaaggt gcggaacgct ctggcgctcg
     8821 actacccgcg cgaccggctc gagctgatcg tggcctcgga cggctcaacc gacgccaccg
     8881 tcgcgcaggc tcgcgcggcc ggcgcggacg tggtgctcga cctgccgcgg gccggcaagg
     8941 ttgccgcgca agatgcggcc gcggaacggg cgcgtggtga gatcctcgcg ttcgccgacg
     9001 ccaactccac ctgggcgcgc gatgccctgc gccgcctggt cgagccgttc gcggacgagc
     9061 gcgtgggcta cgtgtgcggg caggtgcgct tttcgcgcga cgagggcgga acgaacgagg
     9121 agggcatcta ctggcgctac gagatggcgg tgcggggcct cgagtcgcgg gcggccggaa
     9181 tcacggcggg caacggcgcg atctacgccg tgcgccgatc cgcctactgg ctcgacccgc
     9241 tgcggggcga tctctcgttc ccgttcaagc tggcgaagcg cggcctgcgc tccgtctatc
     9301 agcctcaggc gctcgcccag gagaagatgg tgccgacgat cgagggcgag ttccggcgta
     9361 agcggcgcat gatgagccgt gcctggggga cggtgctcga gggcggcatg ctgtcgccgc
     9421 tccgctaccc gccgctgtac gcgtacgaga tcttcagcca ccgcgtcctc cgctacgcgt
     9481 cgccgttcct gcacgtggtc gcgttcggca cgaacatcgc gctgctcggc aacggctggg
     9541 tctacgtgct caccttcgcc gcgcagtgcg ctctgcttct ggcggcggcg ctcgcaccgt
     9601 tcgtgcgtgc ccgcccgttc gcgatcgcct actactacgt gacgatcacc gcctcgattg
     9661 ccgccggcct attcgactgg ctgcggcggg gcatgccgct cacctgggag aagccggagg
     9721 gcacgcggtg atcacgcgcg cgatcgacat cgtcgtcgcg ggcgtcgcgc tcgtgttcac
     9781 gtcgccgatc atcgccgcgg cgatgatcgc catccggctc gagtcgcccg gctcgccgat
     9841 ctacaagcag cggcgcgtgg gattggatgg cgagccgttc gagatctgga agctgcgcac
     9901 gatgtacctg ggatccgatc gcggtcctga ccgtgcgatc ccgaaggacg accccagcat
     9961 cacacgcgtc ggcgctttcc tccgccgcac gtcgctcgac gagctgccga acctgatcaa
    10021 cgtcctgcgc ggcgagatgg cgatcgtggg cccccggccg acgatccagg tgcaggtcga
    10081 ccagtacacg ccgcgccagc ggcggcggct cgaggtgaag ccggggctga ccggctgggc
    10141 gcaggtgaac ggccgcgtcg ggctgccgtg gccggagcgg atcgagctcg acgtctggta
    10201 cgtggaccac cgatccctgc ggctcgacct ccagatcctg gccaagaccg tgcgcttgct
    10261 cgtgacgggt cacggcctct acacgtcctg atcc
//
LOCUS       DASVXS010000226         9758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_810209, whole genome shotgun sequence.
ACCESSION   DASVXS010000226 DASVXS010000000
VERSION     DASVXS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810209"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..461)
                     /locus_tag="VF752_12555"
     CDS             complement(168..461)
                     /locus_tag="VF752_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856420.1"
                     /translation="MDFLLIMVVLVAVAVVVFAPLRSDRSERGEDPEVAALEAERDAK
                     LREIRDFEIDFETGKLSLAEHRALDLTLRAEAAAILRRLDAAREGSRERRVRA"
     gene            complement(464..2614)
                     /locus_tag="VF752_12560"
     CDS             complement(464..2614)
                     /locus_tag="VF752_12560"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX8856421.1"
                     /translation="MITLGRALLLLALALAVGGAAASIYGARTRRPEWVAVGRRSAYA
                     LVGVLTLAFALLEAAFLRSDFALQVVAGHSSTTTPLFYRATAAWSSQEGSLLLWVWLL
                     SLWSSAVLRSSHARLRELAPYATAVLLGFAAFFGSMLVFLANPFQLLDPAPADGSGLQ
                     PLLRHPAMMVHPPLLYSGYTLLTVPFAFGVAALVTRRLDAAWIAATRRFALAAWLCLG
                     AGILLGARWSWSELGWGGYWAWDPVENAALMPWLTCTAFIHSVMVQQRRGLLKTWNMS
                     LVLGSGILAVLGTFLVRSGILDSIHAFGASTLGKPFLAFVAALAFGSVLLVASRRAEL
                     RSEGRDWSLVSRESVFLLNNLVLVGLCLVILWGTFFPLISEAATGDKASVGPPWFDRY
                     TAPLAILAVALSGLGPLLAWRRSSAEGARRALLRPLAASVAVVAVVAAAVPAAAGSPR
                     TLLLFGAAALAIAAVAQELWRGAAARRRVSGEGPARAVVSLVRRNRRRYGGYLVHAGV
                     AVMLVGVAASSAFQHVRDVRLSPGQDARVGSYDVRYVSATGAVGKEKVSLGAVLDVRR
                     DGRHVATLRPNRGYYPSRDPGLGPAGRWFDGEAASEVGLWVRPQGDVWTAVQPDIQSM
                     QRMIDGIDRRFPLADAASERFILQAIADRYRMLPPAASFRLIVSPLVEWVWIGGALAV
                     LGGLISLWPRPRSLRVRSAAWAAAGRLGRALERA"
     gene            complement(2616..3023)
                     /locus_tag="VF752_12565"
     CDS             complement(2616..3023)
                     /locus_tag="VF752_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX8856422.1"
                     /translation="MARDPSRSRKRRLVVALLAAVVLAGALVYTSFSSASSARSPSQL
                     IRSAQPGRSYDVTGKVLPGYTRNGDELRFRLRDRGGSAAIAVLYRGAVPDPFRAGREV
                     IVTVARQDGQFVGRRDSLITKCPSKFSAAPKRT"
     gene            3115..4449
                     /locus_tag="VF752_12570"
     CDS             3115..4449
                     /locus_tag="VF752_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856423.1"
                     /translation="MGASGRLGRYLKIGAAAAGLLLIGGALFSFSAVSKSRHARHATS
                     QQRLFDQGRQVFRFDTFGDQAFWGGGLQLHRAIEGSKLGGVGGGVSPKTALSVGLKVD
                     SQALPKQVVQGIKQGKIDLNDPKTTLALLKLNAVVGVKGFFNHRGTLRSVGLTCAVCH
                     STVDNSFAPGIGKRLDGWPNRDLNVGAIVSLAPNLKPVTDLLGADEATVKKVLAAWGP
                     GKFDAELFLDGKGFRPDGKSAATLIPPAFGLAGVNLHTWTGWGSVPYWNAFVANLEMH
                     GQGNFFDSRLDDASKFPIAAKNGFGHLRVKHDLVTSKLPALQFYQLGLKVPKPSPHSF
                     NAAAARRGKAVFDGQGKCASCHTPPTYTEAGWNLHTPAEVCTDSFQAERSPTGMYRTA
                     PLRGLFTHSKGGFYHDGRFANLGAVVDHYNSCMNLGLTPQQKGDLVQYLKSL"
     gene            4469..5197
                     /locus_tag="VF752_12575"
     CDS             4469..5197
                     /locus_tag="VF752_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEX8856424.1"
                     /translation="MRVVPRKAEGALPGAAAAGLAAAFELVFFYAPLDADQGFIQKIF
                     YLHVPLAIVALSGFVAGGVMGLKYLRTRDPAWDMRSYVAIHLSLILAVGVLVTGSIWA
                     RGSWGHWWVWQEPTLVSFLIVFLLYCCYQPLRFSIEDRERQARYAAVFAVVAAAFVPF
                     NFLAVRLAQAFSHPRVLSTTGGNMPGAMRLTFYVSLVAMALLWLALFKVEISSKRARI
                     ELKRLRREIESEAAVDPREPAMAA"
     gene            5201..5365
                     /locus_tag="VF752_12580"
     CDS             5201..5365
                     /locus_tag="VF752_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856425.1"
                     /translation="MPALPLDTAGAYVAAAYIVFLTLVVLYVGIMASKVARTERDLAE
                     LRRRQQRGER"
     gene            5362..6414
                     /gene="hemA"
                     /locus_tag="VF752_12585"
     CDS             5362..6414
                     /gene="hemA"
                     /locus_tag="VF752_12585"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX8856426.1"
                     /translation="MSERVVCLGLSHRTAPVALRERAALCDRGVEALLSELAAMPAVN
                     EALVLSTCNRTELYGAGPDEGALRAALGCALVGHSRLSAPELARVAYVERGAAAVRHL
                     MRVAASLDSALLGESEIQGQVKHAQRLAALNGSLGPILESSCRAALAAGSRARRETGI
                     ASGAVSTASAAVELARNELEGLLGRRALVIGAGRVADGVAHALRGRGLKEITVASRTD
                     AHARALATRVGGRPIRFPVRLEELAAADVVISATAAPGHVLACAALERAVAARGHGRL
                     VVVDMAVPRDVDPAVNVLSAVTLKDVDDLSRAVDRNLALRRREARVAERIVDAELERV
                     LARIFASDADSLPTAA"
     gene            complement(6411..7166)
                     /locus_tag="VF752_12590"
     CDS             complement(6411..7166)
                     /locus_tag="VF752_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX8856427.1"
                     /translation="MHEPLRWVAGTLARRRSPASYRLRENGRTILIRHRSLDPMTLDQ
                     VFVTDREYEPPPEAAGSIERLGRALRIVDVGANVGLFFLFALGRFPGSTVLAYEPDPE
                     NAALARHCIELNAIGDRVTLIEACAATSAGEVAFAAGKSAASHAATPTESALRVPVVD
                     AMPELTGADFVKIDAEGAEWELLADPRLTGARAAAMIVEYHTRMCPDPDPRAYATRLL
                     ESAGYRTSNPWHNPGAGDDDGMGLLWAWRPARS"
     gene            complement(7247..7618)
                     /locus_tag="VF752_12595"
     CDS             complement(7247..7618)
                     /locus_tag="VF752_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856428.1"
                     /translation="MKRLLVPLAAAAVCAAALPATSPARSACVNLKMSKSTKSLIRAA
                     HKKVDPDAIGPKRGSVYYGRCGNTFYALASFKNPRTGYDDQPEGFRSRGTTAPWRDIG
                     DNGCAKMPKRLLAIWSYDCPF"
     gene            complement(7673..8098)
                     /locus_tag="VF752_12600"
     CDS             complement(7673..8098)
                     /locus_tag="VF752_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856429.1"
                     /translation="MEQRVSVITLGVEDIQRSQRFYEALGWQVGNDSEAQGVAFFQAG
                     GLVFALWGRDELAEDSGVEPSPAGPYGGITLAHNVRSPAEVDRVIGEAEAAGATITRR
                     PGETFYGGYSGVFVDFDGYPWEVAHNPHWTIAEDGSVTF"
     gene            complement(8098..8463)
                     /locus_tag="VF752_12605"
     CDS             complement(8098..8463)
                     /locus_tag="VF752_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856430.1"
                     /translation="MRVLTLVVVTTALLVGCGGGGEKHPSAKEAQACLKKAGATVQAI
                     QPKATGTDSARTELIVRLKDTGALVGFFESEKAADNPSGVKKIQQSGGDVTVKGSTAL
                     VIVPGSGQGGRATIEGCVF"
     gene            8489..9190
                     /locus_tag="VF752_12610"
     CDS             8489..9190
                     /locus_tag="VF752_12610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX8856431.1"
                     /translation="MKVLAVIHDRDAPAGVFGHATRDAGHELEEWFPPDGHEPPALDD
                     LGAVMVFGGVPQVDQEHEHPWLGPEKRWIREAFEGGMPVLGVCLGSQLLADAAGGRAH
                     PAERPEIGWREVTLADGASRDPLLGILPERFRSFQWHSYTFEPPPGVPVLARTPQTAQ
                     AFRAGERAWGVQFHAEVTAATLEAWLRDGADGEDARSIGLDAVAVQRQSQDLIGDWNE
                     LGRQLSSRFLQLAAS"
     gene            9202..>9758
                     /locus_tag="VF752_12615"
     CDS             9202..>9758
                     /locus_tag="VF752_12615"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856432.1"
                     /translation="MRRVIVLGSTGSIGTQALDVIERSPDLEVVALAAYSNFEQLLEQ
                     ARRHGVERIALADERAAARAAESWTEGEVLTGAEGLVGLITDTDCDLVLNALVGSAGL
                     GPTIATLGEGIDLALANKESLVVGGELVMALAEATGSSVIPVDSEHSALKQLIDSERP
                     GTVDRLVLTASGGPFRGRSAAELADV"
BASE COUNT         1471 a         3461 c         3452 g         1374 t
ORIGIN      
        1 gtcgaggcgt gggagggaga ggtttggcgc ggccggtcgc tcgccgccgg cgagcgcgct
       61 gtccaagccg cgccgcggcg agctctgcgt catcccatag ccgagcaggg ccaccagcgc
      121 ccccgcggcg atcagggccg cgagcggggc gggcgcggcc acgcggctca cgcccgcacc
      181 ctccgctcac ggctcccctc gcgggcggca tccagccgcc gcaggatcgc ggccgcctcg
      241 gcacgcagcg tcaggtcgag cgcccggtgc tccgccagcg acagcttgcc ggtctcgaag
      301 tcgatctcga agtcgcggat ttcgcgcagc ttcgcgtcgc gttcggcctc gagggcggct
      361 acctccggat cctcgccccg ctcgctgcga tccgaccgga gcggcgcgaa caccaccacg
      421 gcgacggcga cgagcacgac catgatcaga aggaagtcca tggctaggcg cgttccagcg
      481 ccctaccgag ccgcccggcc gccgcccacg cggccgaccg cacgcgcagg ctgcgcggtc
      541 gcggccacag tgagatcagt ccgcccagga cggccagcgc cccgccgatc cagacccact
      601 cgacgagcgg cgagacgatc agccggaagc tggccgccgg cggcagcatg cggtagcggt
      661 cggcgatcgc ctggaggatg aagcgctcgc tcgctgcgtc ggccaacggg aagcggcggt
      721 cgatgccgtc gatcattcgc tgcatcgact ggatgtcggg ctgcaccgcg gtccagacgt
      781 cgccctgcgg ccgcacccag aggcccacct cgctcgcggc ctcaccgtcg aaccagcggc
      841 cggccggccc aagtcccggg tcccgcgagg ggtaatagcc gcggttcggc cgcagggtcg
      901 ccacgtggcg cccgtccctg cgcacgtcga gcactgcgcc gaggctgacc ttctccttgc
      961 ccaccgcgcc cgtggcgctc acatagcgca cgtcataaga gccgacgcgc gcgtcctgtc
     1021 cggggctcag gcgcacgtcg cgcacgtgct ggaacgcgga ggaggccgct acaccaacga
     1081 gcatcacggc gacgcccgcg tgaaccagat agccgccgta ccgccgccgg ttgcggcgca
     1141 ccagcgagac gacggcccgc gccggtccct cgcccgatac gcgccgccgc gccgcagcac
     1201 cgcgccagag ctcctgcgcg accgccgcga tggcgagcgc cgccgccccg aagagcagca
     1261 gcgtgcgcgg gctgccagcc gcggccggca cggcggcggc aacgacggct acgaccgcga
     1321 cggatgcggc aagcggccgg agcagagcgc gccgggcgcc ctccgcggac gaccgtcgcc
     1381 atgcgagcag cggcccgagt cccgagagcg ccacagcgag gatcgcgagc ggggccgtgt
     1441 agcggtcgaa ccagggcggt ccgaccgacg ccttgtcgcc ggtcgcggcc tcggagatca
     1501 gcgggaagaa ggttccccac aggatcacga ggcagagtcc gacgagcacc aggttgttca
     1561 gcaggaagac cgactcgcgc gacaccagcg accagtcgcg cccctcggag cggagctccg
     1621 cgcgccggct cgccacgagg agcaccgagc cgaacgcgag ggcggccacg aacgcgagga
     1681 acggcttccc gagcgtggac gcgccgaacg cgtggatcga gtccaggatc cccgagcgca
     1741 ccaggaaagt cccgagcacg gcaaggatgc cgctgccgag cacgaggctc atgttccacg
     1801 tcttcagcag cccgcgccgc tgctggacca tcaccgagtg aatgaacgcc gtgcacgtga
     1861 gccagggcat cagcgcggcg ttctcgaccg ggtcccaggc ccagtacccg ccccagccga
     1921 gctcggacca cgaccagcgc gcgccgagca ggatgccggc accgaggcag agccatgcgg
     1981 cgagcgcgaa gcgcctggtg gccgcgatcc acgccgcgtc gaggcggcgc gtcacgaggg
     2041 cggcgacgcc gaacgcgaac ggcaccgtga gcagcgtgta ccccgagtag aggagcggtg
     2101 ggtgaaccat catcgcgggg tgccggagga gcggttgaag cccgctgccg tcggcgggcg
     2161 ccgggtcgag cagctggaac ggattggcga ggaacacgag catcgagccg aagaacgcag
     2221 cgaagccgag cagcacggca gtggcgtacg gcgcgagctc ccgcagacgt gcgtggctcg
     2281 agcggagcac cgcactcgac cacaaagaca gcagccagac ccagagcagc agcgagccct
     2341 cctgcgacga ccaggcagcg gtggcgcggt agaacagggg agtggtggtg ctcgagtggc
     2401 cggcgaccac ctgcagcgcg aagtcggagc gcaggaaggc tgcctcgagc agcgcgaacg
     2461 ccagcgtcag cacgcccacc agcgcgtacg ccgagcggcg gccgacagcc acccactccg
     2521 gccgtctcgt ccgtgcgccg tagatcgagg ccgcggcgcc acccaccgcg agcgcgagcg
     2581 cgagcagcag gagcgcacgc ccgagggtga tcacgtcacg tcctcttagg cgcggcgctg
     2641 aacttggacg gacacttcgt gatcagcgag tcgcggcggc ccacgaactg tccgtcttgc
     2701 cgagccacgg tcacgatcac ctcgcgtccg gcgcggaatg gatccggcac ggcgccgcga
     2761 tagagcacgg cgatagcggc actgccgcca cgatcgcgca ggcggaagcg cagctcgtca
     2821 ccgtttcgcg tgtagccggg gagcaccttg ccagtcacgt cgtagctgcg cccgggctgc
     2881 gcggaccgga tcagctgact cgggctccgg gcgctgctcg cggagctgaa gctcgtgtac
     2941 acgagcgcgc ctgccaggac cacggctgcg aggagcgcga ccacgaggcg tcgcttcctg
     3001 cttctggacg ggtcccttgc cacggctctc ctccgcttga caaagattca tggcactgta
     3061 tataaatcca attcgccgtg tcaattggcc ctcgggtgac ggtgaggagg agcgatgggc
     3121 gctagcggca ggctcggcag gtacctcaag atcggggctg ccgcggcagg acttctcctc
     3181 atcggcggcg ctctgttcag cttcagcgcg gtctcgaaga gcaggcacgc ccggcacgcc
     3241 accagccagc agcggctctt cgaccagggc cggcaggtgt tccggttcga caccttcggc
     3301 gaccaggctt tctggggcgg tgggctccag ctgcaccggg cgatcgaggg atcgaagctc
     3361 ggcggcgtcg gcggcggcgt gagcccgaag accgcgctgt cggtgggcct gaaggtcgac
     3421 tctcaggcgc ttccgaagca ggtggtccag gggatcaagc agggcaagat cgacctcaac
     3481 gacccgaaga caacgctcgc gctgctcaag ctcaacgcgg tcgtcggtgt gaagggcttc
     3541 ttcaaccacc gcggcacgct gcgctcggtc ggcctcacgt gcgccgtctg ccactcgacg
     3601 gttgacaatt ctttcgcgcc agggatcgga aagcgcctcg acggctggcc caaccgcgac
     3661 ctcaacgtcg gcgcgatcgt gtcgctcgcc ccgaatctca agccggttac cgatctgctc
     3721 ggcgcggacg aggcgacggt caagaaggtg ctcgcggcct ggggcccggg caagttcgac
     3781 gccgagctgt tcctcgacgg caagggcttc cgcccggacg gcaagtccgc ggccactctg
     3841 atcccgcctg cgttcggact tgccggcgtg aacctgcaca cgtggacggg gtggggctcc
     3901 gtgccgtact ggaacgcctt cgtggcgaac ctggagatgc acggccaggg caacttcttc
     3961 gactcgcgac tcgacgatgc gagcaagttc ccgatcgccg cgaagaacgg ctttgggcac
     4021 ctgcgcgtaa agcacgacct ggtcacgagc aagctgccgg cgctgcagtt ctaccagctg
     4081 gggctgaagg tgccgaagcc ctcgccgcac agtttcaacg cggcggccgc gcggcgcggc
     4141 aaggcggtct tcgatggcca gggcaagtgc gcgagttgcc atacgccgcc gacctacacc
     4201 gaggccggtt ggaacctgca cacgccagcg gaggtctgca ccgactcgtt ccaggcagag
     4261 cgctcgccta cgggcatgta ccggaccgct ccactccgcg gcctgttcac gcactcgaag
     4321 ggcggcttct accatgacgg gcgcttcgcg aatctcgggg cggtggtcga ccactacaac
     4381 agctgcatga acctgggcct gacgccgcaa cagaaaggcg acctcgtcca gtacctgaaa
     4441 tcgctgtgag cgagggctga gaacggcgat gcgcgtggtg cccagaaagg cggagggcgc
     4501 actccccggc gccgctgccg ccggactcgc cgccgcgttc gaactcgtgt tcttctacgc
     4561 cccgctcgac gccgaccaag ggttcatcca gaagatcttc tacctccacg tgccgctcgc
     4621 gatcgtggct ctctccggct tcgtcgccgg tggcgtgatg ggcctgaagt atctgaggac
     4681 gcgcgacccg gcctgggaca tgcgctcata cgttgcgatc cacctgtcgc tgatcctcgc
     4741 cgtcggcgtc ctggtgacgg gctcgatctg ggcacgcggg tcgtgggggc actggtgggt
     4801 ctggcaggag cccacgctcg tctcgttcct gatcgtgttc cttctctact gctgctacca
     4861 gccgcttcgc ttctcgatcg aggaccgcga gcggcaggcg cgctacgcgg ccgttttcgc
     4921 ggtcgtggcg gccgcgttcg tgccgttcaa cttcctggcg gtgcgcctgg ctcaggcgtt
     4981 cagccacccg cgcgtcctct ctacgaccgg cggcaacatg cccggcgcca tgcggctcac
     5041 cttctacgtg tcgcttgtcg cgatggccct tctctggctc gccctcttca aggtcgagat
     5101 ctcgtccaag cgtgcgcgca tcgagctcaa gcggctgcgg cgggagatcg agtcggaggc
     5161 ggccgtcgac ccgcgcgaac ccgcgatggc cgcctgaccg atgccggcgc tcccgctcga
     5221 caccgcaggc gcgtacgtcg cggcggccta catcgtgttc ctcacgctgg tcgtgctcta
     5281 cgtggggatc atggcgtcga aggttgcgcg caccgagcgc gacctagcgg agctgcggcg
     5341 gcggcaacag cgcggggagc gatgagcgag cgagtcgtct gtctagggct ttctcaccgc
     5401 acagcgccgg tggcactccg tgagcgggca gcgctatgcg accggggcgt agaggccctt
     5461 ctcagcgagc tcgccgcgat gccggccgta aacgaggccc tcgtcctctc gacctgtaac
     5521 cgaaccgagc tctacggggc aggaccggac gagggcgcgc ttcgcgccgc cctcggctgc
     5581 gcgttggtcg gtcacagccg gctgagcgct cccgagctcg cccgcgtcgc ctacgtggag
     5641 cggggcgcgg cggccgttcg ccatctgatg cgggtggcgg cgagcctcga ttcggcgctg
     5701 ctcggggaga gcgagatcca gggccaggtg aagcacgcgc agcggctcgc cgcgttgaat
     5761 gggtctctcg gcccaattct cgagagctcg tgccgcgccg ccctggccgc cggcagtcgg
     5821 gcccggcgag agaccggcat cgcctccgga gctgtgtcca ccgcgtcagc cgcggtggag
     5881 ctcgcccgca acgagctgga gggcctgctc gggcgcaggg cgctcgtcat cggagcgggg
     5941 agggtggccg acggcgttgc gcacgcgctc cgcgggcggg ggttgaagga gatcaccgtc
     6001 gcaagccgga ccgatgcgca cgctcgcgct ctcgcgacgc gggtcggcgg gcgcccgatc
     6061 cgctttcccg tgaggctcga agagctggcg gccgcggacg tcgtcatctc cgccaccgcc
     6121 gcgcccggtc atgtgctcgc gtgcgcggcg ctcgagcgcg ccgtcgcggc acgcggccac
     6181 gggcggctcg tggtggtcga catggctgtc ccgcgggacg tggaccccgc ggtgaacgtg
     6241 ctttccgcgg tgacgctcaa ggacgtcgac gacctctcgc gcgcggtgga ccgcaacctg
     6301 gcgctccgcc ggcgagaagc ccgcgtggcc gagcggatcg tggacgcgga gctcgagcgc
     6361 gtgctcgccc ggatcttcgc ctcggacgcc gattcgctgc ccaccgcagc ttaggagcgt
     6421 gcgggccgcc aggcccagag cagtcccatg ccgtcgtcgt cgccggcgcc ggggttgtgc
     6481 caggggtttg aggtgcggta cccggccgac tcgagcagcc gcgtcgcata cgcccgcggg
     6541 tccggatcgg ggcacatccg cgtgtggtac tcgacgatca tcgccgcagc ccgcgcgcca
     6601 gtcagccgag gatcagcgag cagctcccac tcggcgccct cggcgtcgat cttcacgaag
     6661 tccgcgcccg tgagctccgg catcgcgtcc acgacaggca cgcgaagcgc ggactccgtg
     6721 ggtgtggccg cgtgcgaggc cgcggacttc ccggcggcga aggccacctc acccgccgac
     6781 gtggccgcgc aggcctcgat cagggtgacg cggtcgccga tggcgttcaa ttcaatgcag
     6841 tgccgcgcca gcgcggcgtt ctccgggtcg ggctcgtagg cgagcaccgt cgacccggga
     6901 aaccgcccga gcgcgaagag gaagaagagg ccgacgttcg cgccgacgtc gacgatccgc
     6961 agcgcccggc cgagccgctc gatcgagccg gcggcctcgg gcggaggctc gtactcgcgg
     7021 tcggtgacga acacctggtc gagcgtcatg gggtcgagcg agcggtgccg gatgaggatc
     7081 gtgcggccgt tctcgcgcag ccggtaggac gcgggcgatc gccgccgcgc gagcgtgccc
     7141 gccacccagc ggagtggctc gtgcaccgcg gcggcgtgcc gcaccaccag cgccggcggg
     7201 ctgcgcttgc ggaggcggcg gagcctggcc gatccgcgga tgcgcgtcag aagggacagt
     7261 cgtagctcca gatggcgagc aggcgcttcg gcatcttggc gcagccgttg tcgccgatgt
     7321 cccgccacgg cgcggtcgtg ccgcgcgagc ggaagccctc gggctggtcg tcgtagcccg
     7381 tgcgcgggtt cttgaaggac gcaagcgcgt agaaggtgtt gccgcaccgg ccgtaataga
     7441 cggagccgcg cttcgggccg atcgcgtcgg ggtccacctt cttgtgtgcg gcgcggatca
     7501 gcgacttcgt ggacttcgac atcttcaggt tcacgcaggc ggacctggca ggcgatgtag
     7561 cgggcagcgc ggcggcgcat acggcggccg cggcgagtgg gacgaggagg cgcttcatag
     7621 agcctttcta gcgggcctcg gccgctcgcg ccacttggac cgcgcccggc gcctagaacg
     7681 tcacgctgcc atcctcggcg atggtccagt gcgggttgtg ggcgacctcc cacggatagc
     7741 cgtcgaaatc gacgaacacg cccgagtagc caccgtagaa cgtctccccg ggccttcgag
     7801 tgatcgtggc gcccgcggcc tctgcctcgc cgatcacccg gtccacctct gccggcgagc
     7861 gcacgttgtg agcgagcgtg atgccgccgt acggcccggc cggcgagggc tccaccccgc
     7921 tgtcctccgc gagctcgtcg cggccccaga gcgcgaagac caagccgccg gcctggaaga
     7981 acgccacgcc ctgcgcctcg gagtcgttcc cgacctgcca gccgagcgcc tcgtagaagc
     8041 gctgcgaacg ctggatgtcc tcaaccccga gggtgatcac gctgacccgt tgctccatca
     8101 gaacacacac ccttcgatcg tcgcgcggcc accctggccg cttcccggca ctatcacgag
     8161 cgctgtgctg cccttcacgg tcacatcacc gccggactgc tggatcttct tcacgcctga
     8221 cggattgtct gccgccttct ccgactcgaa gaagcccacg agcgcgcctg tgtccttcaa
     8281 ccgcacgatc agctcggtgc gggccgagtc ggtgcccgtc gcctttggct gaatcgcctg
     8341 caccgtcgcg ccggccttct tcaggcaggc ctgcgcctcc ttggcggacg gatgcttctc
     8401 gccgcccccg ccgcagccga cgagcagggc cgtcgtgacg actacgaggg tgaggacgcg
     8461 catgcgagga caataaggtc acgtggcgat gaaggtgctc gccgtgattc acgaccgcga
     8521 tgctccggca ggcgtgttcg gccacgccac gcgcgacgct ggtcatgagc tcgaggagtg
     8581 gttcccaccg gacgggcacg agccgccagc actcgacgac ctgggcgcgg tgatggtgtt
     8641 cggcggcgtt ccccaggtcg accaggagca cgagcacccc tggctcgggc cggagaagcg
     8701 ctggatccgc gaggcgttcg agggcgggat gccggttctc ggcgtctgcc tcggctcgca
     8761 gctcctggcc gacgccgccg gcgggcgggc gcacccggcc gagcgacccg agatcggctg
     8821 gcgcgaggtg acgctcgcgg acggggcgtc ccgcgacccg ctgctcggga tccttcccga
     8881 gcgctttcgc tcgttccagt ggcacagcta cacgttcgag ccgccgcccg gtgtgccggt
     8941 gctggcgcgc acgccgcaga ccgcacaggc cttcagagcc ggcgagcgtg cctggggagt
     9001 gcagttccac gccgaggtga ccgcggccac gctcgaggcc tggctgcgcg atggcgcgga
     9061 tggggaggac gcccgctcga tcggcctgga tgccgtggcg gtgcagcgcc aaagccagga
     9121 tctgatcggc gactggaacg agctcggccg gcagctctcg agccgttttc ttcagctcgc
     9181 cgcctcctag actcaggtca catgcgcagg gtcatcgtcc tcggctcgac cggctcgatc
     9241 ggcacgcagg cgctcgacgt gatcgagcgc tcacccgacc tcgaggtggt cgccctcgcc
     9301 gcgtactcga acttcgagca gctgctggag caggcgcgcc ggcacggggt cgagcggatc
     9361 gccctcgccg acgagcgcgc cgcggcgcgc gcggcggagt cctggaccga gggcgaggtg
     9421 ctcacgggcg ccgaggggct cgtcggcctg atcaccgaca ccgactgcga tctcgttctc
     9481 aacgcgctcg tcggctcggc aggcctcggg ccgactatcg ccacactcgg tgaggggatc
     9541 gacctggcgc tcgccaacaa ggagagcctc gtggtcggcg gcgagctagt gatggcgctc
     9601 gccgaggcca ccggatcgag cgttatcccc gtcgactccg agcactcagc gctcaagcag
     9661 ctgatcgact cggagcgccc cggcacggtc gaccggcttg tgctcacggc ttcgggcggt
     9721 cctttcaggg gccgcagcgc cgccgagctc gccgacgt
//
LOCUS       DASVXS010000227        35403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_488758, whole genome shotgun sequence.
ACCESSION   DASVXS010000227 DASVXS010000000
VERSION     DASVXS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 35403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35403
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488758"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF752_12620"
     CDS             <1..339
                     /locus_tag="VF752_12620"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8856433.1"
                     /translation="ELRRRAVLPLLVLHYIQAGPSYGNQLMERISDATAGVLSVNPNT
                     MYPLLRQLEARNLIRGEWEHPERRSRRYYSITDEGRAEYQRLETEVRPFLESVARSID
                     AIVSEVYGER"
     gene            336..815
                     /locus_tag="VF752_12625"
     CDS             336..815
                     /locus_tag="VF752_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856434.1"
                     /translation="MSIARADGPVEVPAVTAAALWTNPDRWATFVEGFAHVNEKRDGW
                     PEPGGKIVWRSIPGGRGIVTERVLEHTRDDDGSTRIVSRVFEEALTGTQTATFTQTDV
                     GCEMALELEYELAPNTVMRTGVLGKITNALFIRRALSDSLARTIRRFTTEAHEEASL"
     gene            854..1729
                     /locus_tag="VF752_12630"
     CDS             854..1729
                     /locus_tag="VF752_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8856435.1"
                     /translation="MFVFKAAVVGAGTMGGEIAQTIASSGIAVVLKDVEQKFVDQGLE
                     KARQVTQGQLGGLVNKEKITQEQADAQLEEIVGRVTGTTDYEGFGDVDFVIEAVPERM
                     PIKQSVFADLDAVTPGHAILSSNTSSLSITEIGEATSRPDKVVGFHYFYPASMMRLIE
                     VIEGDETSEETLGAARNFAMQIGKTPIVCGEVPGFVVNRILNSAVAEVWRVQEGEGID
                     YKEIDRVVQESKTVPMGPFFLTDLLGLDTVLHVAEHLKESYGDRYYVHSGMKELVEQG
                     NLGAKTGKGFYEHGS"
     gene            1713..2816
                     /locus_tag="VF752_12635"
     CDS             1713..2816
                     /locus_tag="VF752_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8856436.1"
                     /translation="MSTAADQTVTGTLVERFGLKALVEACLVLEEGVASLKDIEIGMM
                     MGAGILPGPFARADEQGLDEVLAALERAERDWGEYFAPPLILRRLVNQGRLGKKTGQG
                     FFPYAQPDEGQSRETIALETRGDIAIVWLNRPPANPLSPQVVRELAELWSELDGNARA
                     VVFASSNIFTFSAGADIKEFTRMSPDKKGRELLESAHGFMRAMERSRTVTIAAVNSLA
                     LGGGCELAMACDFRLAAESATFGQPEINLGIIPGFGGTQRLPRLVGEGKALEMNLTGT
                     PIGAYEAYRAGLANQVVPDHELFDTALMWARKMSEQAPIAISKIKTVAAQGDLDEGLR
                     LEGEAFGEAFGSEDAKEGIGAFLGKRRPRFQGK"
     gene            2813..3586
                     /locus_tag="VF752_12640"
     CDS             2813..3586
                     /locus_tag="VF752_12640"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX8856437.1"
                     /translation="MTTETGASARDAARVAELLTRSHRAVVLTGAGVSVPSGIPDFRT
                     PGKGLWENVDPMEVAHIDVFRRDPDRFWHFYGSRFASLGDKRPNRAHEIIAELEERGL
                     VRAVITQNIDRLHRRAGSETVIEVHGSIEVCVCPECGAKVGIDRVLEQLEAGVGTPEC
                     EVCIRPLKPDVVLFGELLPERAMAEAYALASEADLMLCIGSSLEVYPVASLPTVTRDA
                     GGKLAIITQGSTPYDREADAKLEGDVVDELEDVLEALDL"
     gene            3592..3942
                     /locus_tag="VF752_12645"
     CDS             3592..3942
                     /locus_tag="VF752_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX8856438.1"
                     /translation="MLVALGGAAGVLARYGLGTAVPNDARPWLTVAINVSGSFLLGLL
                     VTVGDWFSAEVRTALAVGVLGGFTTFSTFSVDVFTDIESGRGGEALLYLGLSVVLGVA
                     AAAAGYYAGRGLAH"
     gene            complement(4351..4539)
                     /locus_tag="VF752_12650"
     CDS             complement(4351..4539)
                     /locus_tag="VF752_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856439.1"
                     /translation="MSDAIPIQNARRRSGRRSDDRPVARAIEARKSLGTGRGRVWLNG
                     SEVGGTDPRFSHLAASYD"
     gene            complement(4695..5564)
                     /locus_tag="VF752_12655"
     CDS             complement(4695..5564)
                     /locus_tag="VF752_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856440.1"
                     /translation="MAAQGSFAFVQLEFGFVLGPDDGRYVTRAGPGAAPERVVVISTL
                     NAPKPAPRLSRRRRPKQVTHAATPAMPLARVTMIHAGPFPAPADAASWLGALVTDSEA
                     LHFEVDLAVRELNRVLRAHRAAAADPYARDVSAAQAIAVRVGYGDGSEVADGNYTDAY
                     EVPRDERRRKRSEMLLPNERLAAVLAGREDILASEELVLRARADVDAGRHREAALQTR
                     VALEALLAELGPDELGDRRAEVAARRPLVADAANAALAGDVPSELSEGVAETVTLLES
                     ALARARAKQASGS"
     gene            complement(5581..8145)
                     /locus_tag="VF752_12660"
     CDS             complement(5581..8145)
                     /locus_tag="VF752_12660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX8856441.1"
                     /translation="MRKSLLYILLSAGLVLAVFTGLSFPAEAQDATGSQLPVPTVPST
                     GTPLDGITIPTDTTPVPTPTVPLPTPTVPTVPGVPTLPGGGGGSGGGGGSTGGGTTGG
                     GGGAGGGGSTGGAGGVTGTGPNAEVPKVPGAPNTRAGHRQRRSGAERLKARRKAERDK
                     NTPKTPAGNPIRRPDGVPTPHNPTFFDALPGPAQVSGVPNFVISKFRVPPFLLPIYQA
                     AGIQYGIRWEILAGINEIETDYGRNLNVSSAGAVGWMQFMPSSWKMYGTDANKDGRRD
                     PYNPVDAIFAAARYLKAAGGDQDIRRAIFAYNHADWYVDSVMLRAKLIAGVPPDVIGS
                     LTGLTEGRFPVYAHARYADDITEAQAEKKIKKGHNAARLVGSDSNRKGINIFARRGAP
                     VVATNDGVIKKMGRSKKLGLFVTLQDAYGNQYTYSGLSSLARVHPVPKDTGKVTKTSA
                     NAVPANQKLPKPTQPASAGRQVTKASKKQAAKANAKRSKQLARKQAAKQRLAAHGKSS
                     GQGAATPTVITKQRLFANPSRAGAKRHGGLEQIFNSRSAGAGYTTFRNYFSRALGLNS
                     KNATLRRLHKGSHVVGGTILGRIGKTDAKLAPHVYFQIRPAGKGAPNIDPKPILDGWK
                     LLEATAIYRASGRNALYSSSGDNFSIGQVLLLPKPLLERRVLSDGRIDLYAAGRNDIR
                     TGQIDRRVLATLEYLAESGLRPTVTSLKSGHGFYTASGNVSEHSSGNAVDIAAVNGIP
                     IAGHQDPGGITETTVKRLMMLQGTMRPHQIISLLDFGQNTLALPDHYDHIHVGFQPLF
                     GTNAKAGKDAMAVLKPGQWDDLVKRLGEIQNPVVPTKPSKFALPVHNGRRTGTSGD"
     gene            8540..9022
                     /locus_tag="VF752_12665"
     CDS             8540..9022
                     /locus_tag="VF752_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856442.1"
                     /translation="MAEHEHDEGLVDRLSRQGEEALGKVAEQLLSNPAVAGAISRAFE
                     AREKASQAQEAAMGALGIPSAADIERLTRRLRSVSQRLESIEDSVDRLDERLEAIDRR
                     IGEVAGKAGSSDEVESTLSDISDRLDRLARDLAALRDAVAPGSEPPPRAQERLTVSDG
                     "
     gene            complement(9009..9713)
                     /locus_tag="VF752_12670"
     CDS             complement(9009..9713)
                     /locus_tag="VF752_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856443.1"
                     /translation="MPAASAPEPIHLKPNAELAPRVLLPGDPHRALAVATDLLESPKM
                     FNHHRGLWGYTGPAADGELLTVQSTGMGGPSAAIVVEELIALGAETLVRIGTCGAFGG
                     GLALGDVFAVSSALPDDGASRALGATGPIAPDAALERSLAEAAGREGAVVSTDLFYDT
                     RDGRMAGWLDAGAIAVEMECATLFTLAALRGVATGAVLAVSDEIAAGRVRIDREALEE
                     VGLALGRTAYAALSRR"
     gene            9745..10065
                     /locus_tag="VF752_12675"
     CDS             9745..10065
                     /locus_tag="VF752_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856444.1"
                     /translation="MAKRPTQPVEEALRDAVERTVQATIGSAQDSRTRAQQAVDEIVK
                     SAEAGATAVRERVRGAFEGQLPATHDDVRELQKELRAIVRRLDEIEKRLPKQRSSSGT
                     RKKS"
     gene            10065..11054
                     /locus_tag="VF752_12680"
     CDS             10065..11054
                     /locus_tag="VF752_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8856445.1"
                     /translation="MGRRVLITGVGSFLGTELARRLETDPEFDFVAGVDDRAPRARLE
                     RTEFVEADIRSPIISRIVPQLGVDTIVHNKIVRRPGPTLSARAAHDINVIGTLQLLTA
                     CEKADTLSSIVIRGSAGIYGSEPAAPQFFTEDMSRLYPLRTRFQRDVAEIENYFETFS
                     RRHPAITCTMLRFEPAIGPTLDTQITRYLSLPIVPTYFGFDPRLQFVHEVDAIEATVA
                     AIKRPVRGAVNVAGAGTVGLTKLVRAAGKLTLPIVSPLFGGVSAVAKRLGLLDFSDDF
                     VRLLHYGRAVDITRLTEEVGFTPAYSTPDAVEDYVRTLGGRRLAPPLRQVVSL"
     gene            11051..12097
                     /locus_tag="VF752_12685"
     CDS             11051..12097
                     /locus_tag="VF752_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8856446.1"
                     /translation="MSTETRGPRTRSKRAAPDGGVSPGTDQSRVGSSGAPRGSFAFGP
                     AIGFLRRLRGALGPRVDLPQAFAEATLALPREVREAIDAGRRRLAGDYQEDEWGFDEE
                     FAEAVRPFFDFLYDAWWRVEAKGVSNVPSHGRALLASNHAGILPWDATMISTAILREH
                     PLPRYPRFLVLNWAFELPYVSVLIRKVGGVVASPYNAMRLLEQDELVAVFPEGVKGTG
                     KHFRDRYRLQRFGRGGFVEIALRTGSPIVPVAVVGSEEIYPKIGEAPLLARAVGAPFF
                     PITPTFPALGPLGVVPLPSKWRIEFCDPIETAHYGPDAAENRSLVFELSERVRDTIQQ
                     TLYENLVERGSAFL"
     gene            12134..12595
                     /locus_tag="VF752_12690"
     CDS             12134..12595
                     /locus_tag="VF752_12690"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 and FHA domain-containing protein"
                     /protein_id="HEX8856447.1"
                     /translation="MRASDDTRERAARRLREGCADGRISVDTLSARLDRLFAARDRGE
                     VQAVVADLPRRRTLASLFRRAQRLVPPLVPDGETLTLGRGSDCSLPLDDPFVSRRHAE
                     LSRSDRGWLLRDLGSTNGVWVNGWRVRESELRDGDEVRLGYTRFRFGPGSR"
     gene            12644..13075
                     /locus_tag="VF752_12695"
     CDS             12644..13075
                     /locus_tag="VF752_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8856448.1"
                     /translation="MGFKSPAEFREVMDQVFTIMSTDSEMGPKLRDARTPQRFEFPDL
                     DLVVNITYVEDAEAAANGQNLRWEWSDNVDWEPEVQMTMDSDVANRYFQGKENIAMAI
                     ARRRIKTSGNVKKALNLIPITKPVFERYRAMLAKDHPDLLE"
     gene            complement(13623..15632)
                     /locus_tag="VF752_12700"
     CDS             complement(13623..15632)
                     /locus_tag="VF752_12700"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8856449.1"
                     /translation="MNYFVTGATGFIGRHLVQELLKRDGTVYALVREGSRGRLDDLRK
                     SLGAEDRIVPIVGDLSKEKLGMESFDEKIDHFFHLAAVYDMEADDESMQRANVEGTRN
                     TIQFANSIDLGRFHHVSSIAVAGRYKGLFREDMFDEGQPLPHAYFQTKFESEKLVREH
                     VEAPFRVYRPGIVIGHSETGEIDKIDGPYYFFKLIQKLRHALPEWFPLAGPEGKKANI
                     VPVDFVAKALDHIAHQEDDDLPGNTFHLVDPKPLSAGQVLNEFAKAAHAPRFAMRVDQ
                     NLTNVLPKQVRAGLMAVPTIKGIRDEVLDDLGIPPVVLEYRDFRCDFDARDTQRALKG
                     TGIAVPPLSTYAPKVWDYWERNLDPDLFRERSLSKSVEGKKIVITGASSGIGAETARK
                     IGEAGGEVILISRTREKLDAVAAEVEEAGGIAHVHPADLSDLEDIERVAQEILDEYGG
                     VDVLINNAGRSIRRSIAASYDRFHDFERTMQLNYFGALKLIMAFLPGMRERKKGHIIN
                     VSSIGVQTNTPRFSAYVASKAALDAFSRCMAPEVVGDNVSVTTVYMPLVRTPMIAPTS
                     IYDAFPTLSPDEAAQLIADAIIDKPKRVATRLGTFGQVLYALSPKVVDQIMNTAYKIF
                     PDSKAAKGDKEKAAAGAGSDGEKPKDGEMSTEAVAMAYLLRGVHF"
     gene            15905..17140
                     /locus_tag="VF752_12705"
     CDS             15905..17140
                     /locus_tag="VF752_12705"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8856450.1"
                     /translation="MTAEAANARSTPAPSGGLTLREVAEEVGVTPATLRRWVNQGVVP
                     GETDGRPWPPAAVAHARIVARLRERGHSLQKIKEANDEGRLAFGFLEDLFPTPKRRRT
                     LREVAKETDLEPELIKRIWGGIGLPRPESDDDPEFTDEDVQALQYVSAVLASGFPLVA
                     FLQLVRVYGQAVSQMADAETRLFHLYVHEPLMREGVPGLEMAEEMEHLARDLLPLSSP
                     IMDFIHRRFLQHFVEQDVIGHMEVDFDDESELGRVRVVIAFCDLAGYTRYTEEMGEEE
                     ALSYVERFIDAVTETLPEDARVVKTIGDEVMIVGSDVPALVDWAVGFQRLFDDRPAPR
                     IGIHTGAAIYRDGDYFGREVNLAARVVARARGGEVLITDTVQELVKDSPHLTFEPVGE
                     VKLKGFDEPRSLCRAHSAE"
     gene            17137..18432
                     /gene="tilS"
                     /locus_tag="VF752_12710"
     CDS             17137..18432
                     /gene="tilS"
                     /locus_tag="VF752_12710"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX8856451.1"
                     /translation="MSALDAARESGLIVAGEPLLVMLSGGADSVCLLDVARELGADVS
                     ALHVNHGLRPEADDDEAFCVELSGRLGVPLAVEQARPPGRSNLQAEARDLRYALAERH
                     RGRGDYAAAHTATDQAETVLYRLAVSPGRRALLGMPARRGHLVRPLLEATRAETHAWC
                     EQRGLAWREDRSNVDPRFARARVRREVLAVLSELNPGAERNIAETADLLRDESEVLDT
                     AADEALARIGGEAAVPLAELRALPRALARLMLRRMAERAAGGQVALSRRDADAVLHLG
                     DRGGTRSLDLGGGLRAVAEYGTLRFTREAPEGEQPPPEPVLLTVPGSARFGPWEVVAE
                     LGPGGEAELAGAELGTAVTVRAWREGDRMRPAGLGGSKSLQDLFTDRKVPRGMRRSLP
                     VVEAEGEIAWVAGVAIGERFRAVPNADEVVSLSARPAGT"
     gene            18443..18976
                     /gene="hpt"
                     /locus_tag="VF752_12715"
     CDS             18443..18976
                     /gene="hpt"
                     /locus_tag="VF752_12715"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX8856452.1"
                     /translation="MNNSSIGETLVAEDDLRRRIRELGQEITKDYQGRDLMLVCVLKG
                     AVFFLSDLMREIDLPCEVDFMAVASYGSSTDSSGVVRILKDLDTPLDGRDVLIVEDIV
                     DSGLTLSYLLRMLNARKPASLEVCALLTKPERREVDLPIRYVGFEIPNRFAIGYGLDH
                     VERYRNLPYVASLADPA"
     gene            19094..21064
                     /gene="ftsH"
                     /locus_tag="VF752_12720"
     CDS             19094..21064
                     /gene="ftsH"
                     /locus_tag="VF752_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8856453.1"
                     /translation="MSRFFKSAAFPILIVVVLAFFAQRLISPGDHTPTPTYSDFLTQV
                     DQNQVKDVTVNTKDNTIDVTPVGPNAKKYSTGYPDNTEPQITNLLREHKVPIDVKGKG
                     SSGWLSVLTYVLPFVIFLLFWLFIINQMQGGGSKVMSFGKSRAKRMSVDSPKITFRDV
                     AGVDEAVEELHEIKEFLENPKKFQALGARIPKGVLLYGPPGTGKTLLARAVAGEAGVP
                     FFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFEMKDNIILIAATNRPDILDPALLRPGRFDRQIVVDRPDRKGRQ
                     KILEVHTRGKPLAKEIDLDNLAAQTPGFTGADLANLVNEAALLAARQGSREITHVHLE
                     EGIMRVIAGPEKKTRVMSEKERKITAFHEMGHAIVAHFLENTDPVHKISVISRGQALG
                     YTISLPTEDKFLTTRAELSDTMAMTLGGRAAEEIVFGEVTTGASNDLEKVTATAKQMV
                     MRFGMSEKLGPRVFGHDHGQPFLGREFSAEPDYSDDVAREIDDEIRRIVEEAHQVARD
                     ILMRYRKQLDNVSDILMRRETIDRDQFLQLLDGKTEDDVFGAEEPELPEPTPPAPETP
                     KRKPGIEPRPLPRPGLAGAEMRSDEPKKPEIS"
     gene            21083..21919
                     /gene="folP"
                     /locus_tag="VF752_12725"
     CDS             21083..21919
                     /gene="folP"
                     /locus_tag="VF752_12725"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8856454.1"
                     /translation="MSAHTRPLVMGVVNVTPDSFSDGGMFLDADRAISHGRLLASEGA
                     DYLDVGGESTRPGAKPVSEPEELRRVLPVIEQLVDEGAPPISIDTMKSRVARTALEAG
                     ALVVNDVSAFRLDPEMASVVAEAGVDCCLMHMLGEPRTMQEDPRYDDVVSEVKAFLEE
                     RLAFALSQGIAEDSVWLDPGIGFGKTVDHNLELLRRLDEIAAIGRPLVIGTSRKSFLG
                     KITGRDVGERLAGTIATNVLAYEKGARIFRVHDVAPIVDALEVAAATVRGKWTTSAST
                     RT"
     gene            21889..22323
                     /gene="folB"
                     /locus_tag="VF752_12730"
     CDS             21889..22323
                     /gene="folB"
                     /locus_tag="VF752_12730"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX8856455.1"
                     /translation="MDDEREHEDLEPDEDEDEGEDELEPAVSVEITGLSVYTHHGVEQ
                     AERDVGQRLVFDISFELDHCDATVTDRIEDTVDYADVCQQVALAAQARSYATLERLCS
                     AICDRLLDRYETEWVTVRATKPEPPIPLPVDEVSVEVSKQAG"
     gene            22364..22795
                     /locus_tag="VF752_12735"
                     /pseudo
     CDS             22364..22795
                     /locus_tag="VF752_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
     gene            22792..23277
                     /gene="folK"
                     /locus_tag="VF752_12740"
     CDS             22792..23277
                     /gene="folK"
                     /locus_tag="VF752_12740"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX8856456.1"
                     /translation="MRAYLGLGSNVGDRLDNLRAAVAALEARGIHVVGGSSVYETEPQ
                     GEVRDQPDFLNAAVAVETELGPEELLDECKAVEANLGREPGGVRHGPRAIDVDLLLLG
                     GLEHRSERLTLPHRDVLARRFVLVPLLELDPELALPNGTRLADALSALEGQRVERVGR
                     L"
     gene            23317..24087
                     /locus_tag="VF752_12745"
     CDS             23317..24087
                     /locus_tag="VF752_12745"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX8856457.1"
                     /translation="MLLAIDNGNTQTHVGMFRDGQLVEHWRLATVRESTADELAAWLS
                     GLLNLRGLSLDDVDGAIVSSVVPQLEHEYGLVSERYLKGSLIVIGPKIKTGMPIRMDN
                     PHELGADRLMNAVAAYAKYQSACIVVDFGTAINFDIVSGKGEYVGGVLAPGVEISMDA
                     LAARAAKLPKVDLEPPPEVIGRSTVTALQSGSIYGFSGMVDGVLRRVREALGEPATTV
                     ATGGHAAAIGPHCEEIDELDDLLTLTGLRLVWEKNRHG"
     gene            24101..25087
                     /locus_tag="VF752_12750"
     CDS             24101..25087
                     /locus_tag="VF752_12750"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEX8856458.1"
                     /translation="MNRSLDTPWTLGGIELPNRLVLAPLAGIGNWFVRLQAKRYGAGL
                     VVSEMVSSFGLKYGDERTHREFLRLHPDEHPMAVQLFGHDAAVMREAAAIVAEAGADI
                     IDLNFGCPVRKVCKTGAGSALLDDPDQGVAIARAAVEGGGGLPVSVKIRSGNRPGERT
                     GVELAQRLVHDAGVSAIGFHPRHASQQHKGRPDYALASELVERLPVPVMLSGGLSDED
                     ATLAAFEASGADAVMLARGSLGNPWRFERLLGRRQTPPATEEVVEELLWVIDQAEDHL
                     GVERAGRYLRKFYPWYADTLGLNKRERQDLVTAATTDDARAALRSLSTPIAA"
     gene            25188..25667
                     /gene="greA"
                     /locus_tag="VF752_12755"
     CDS             25188..25667
                     /gene="greA"
                     /locus_tag="VF752_12755"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8856459.1"
                     /translation="MAKDVILTPAGLEDLKAKIDYLSNDRRREVAERIKEAREFGDIS
                     ENSEYDDAKNEQAMLEKQIADLEEKLRNATVIDEKTVSTEAVGVGNLVHVKDQKTDKS
                     VKYQIVGSAEANPSENKLSNESPVGKALLGHKRGDVVSVPVPRGPARKLKITKIETA"
     gene            25801..27255
                     /gene="lysS"
                     /locus_tag="VF752_12760"
     CDS             25801..27255
                     /gene="lysS"
                     /locus_tag="VF752_12760"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX8856460.1"
                     /translation="MTDAGHNPLDDRRAKLERLRDDGVNPFPHEYPGVVPTSIVRAEQ
                     GGLEPGEETDAKYRVAGRLAARRGHGGAAFLDLVDRSGRIQLHAQRDRLGEESFERLV
                     SLDLGDIIGVDGTAFMSRRGELTLRIEDWALLAKSLRPPPEKFHGLEDVETRHRRREL
                     DLMANEETRELFILRSRVIAAIRRWLDERGFLEVETPVLQPLYGGALARPFVTHHNQL
                     NRDFYLRIATELYLKRLIVGGLERVYEIGKDFRNEGISHKHNPEFTMLEWYEAYVDYG
                     DIAAELEDLVSYVANEVGYEGEIDFTPPWRRITLRDAILEKTGIDVLANRNRDGLLAA
                     AAEKGISLNPNETWGQLVDELLSKYVEPELTQPTFILDYPVELSPFAKAHRTEPGLVE
                     RFEAFAAGMEFANAFTELNDPDDQRARFEEQVRHTAEGDEEAQPYDEDFVRALEQGMP
                     PTGGIGVGIDRLVMVLSGRQSIRDVVLWPAMRPE"
     gene            27490..30099
                     /locus_tag="VF752_12765"
     CDS             27490..30099
                     /locus_tag="VF752_12765"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX8856461.1"
                     /translation="MFERFTERARQVVVLAQEEARILKHNYIGTEHILLGLLREEEGL
                     AARVLESLDITVERVRAQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSEKIRNEVIRMLSGPGGRRQGQQGGQQGEA
                     KKSSKLLDQFGRNLTKLAAEGKLDPVVGRETEIERIMQILSRRTKNNPVLIGEPGVGK
                     TAVVEGLAQRITNGDVPELLRNKQIYTLDLAALVAGSKYRGEFEERLKKVMKEITQRG
                     DIILFIDELHNLVGAGAAEGAIDAASILKPALARGELQTIGATTLDEYRKYLERDSAL
                     ERRFQQIRVDQPSTEETVQILKGLRDRYEQHHRVAITDEALEAAAELADRYISDRFLP
                     DKAIDLIDEAASRARIKSMTAPPVYRELEDDIENTRREKEAAIEAQEFEKAANLRDQE
                     RQLTNKKRELEEQWRQGESGNRPDIGEEEIADIVSMWTGIPVFKLTEAETAKLLRMED
                     ELHKRVVGQQAAIEAVSKAIRRSRAGIKDPKRPTGSFIFLGPSGVGKTELARTLAEFL
                     FGDEDAMVRIDMSEYMEKHSVSRLVGSPPGYIGYDEGGQLTEAVRRKPYSVLLLDEIE
                     KAHPDVFNILLQILEDGRLTDAQGRTVDFRHAIVIMTSNIGASEIARNTPLGFAVSDD
                     ETGITYDDMKNRIMGELKKVFRPEFLNRIDEVIVFHKLSKDEVKSIVELLLRHIRQSM
                     AERELSLNLSEDAKDLLVDKGWDPAMGARPLRRAIQRYIEDPVADEVLRSTMEPGSTV
                     QVDKAGDDEDPPVKISITAPTKKREAVGVGAKGDGNEGGSLPDEPDVLPEVPDAPPAE
                     EPPTGEGDTSDEG"
     gene            30177..30404
                     /locus_tag="VF752_12770"
     CDS             30177..30404
                     /locus_tag="VF752_12770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8856462.1"
                     /translation="MAPKTTKASERLAWAVDALDVQPRDRLLEVGCGHGVAVSLACER
                     LDGGRITAVDRSPKMIEMAWRPCTSPEERWR"
     gene            30365..30604
                     /locus_tag="VF752_12775"
     CDS             30365..30604
                     /locus_tag="VF752_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856463.1"
                     /translation="MAALHQPGRALEVVKERLAPGGRLYLFNQEPGWKTPEPAEQFGA
                     RLGDVLSSAGFGSERVVVKDFGGAAVAGVVACSGR"
     gene            complement(30612..30842)
                     /locus_tag="VF752_12780"
     CDS             complement(30612..30842)
                     /locus_tag="VF752_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856464.1"
                     /translation="MAGPVNLIAHFADGPVAGRMELLEARMPTRKVWAPRGWLRRTLK
                     RGPRTANYTLERSWLQDGEHHLLYRFAGTSRA"
     gene            31070..31303
                     /locus_tag="VF752_12785"
     CDS             31070..31303
                     /locus_tag="VF752_12785"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX8856465.1"
                     /translation="MEPQVEIKTRLNGPYKVTGPVRIIDADGNEFDLAEHGEVIALCR
                     CGGSSTKPFCDGTHKRNGFAAAEKAVVTGAAEG"
     gene            complement(31555..31740)
                     /locus_tag="VF752_12790"
     CDS             complement(31555..31740)
                     /locus_tag="VF752_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856466.1"
                     /translation="MTARRGWLALGALGVALLVPFEFPVTVTLGIIALLGFVAWGVFL
                     IATPEFLRRGEDEERNS"
     gene            complement(31737..32768)
                     /locus_tag="VF752_12795"
     CDS             complement(31737..32768)
                     /locus_tag="VF752_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX8856467.1"
                     /translation="MAETCLILALATLTAYVVLGGADFGGGFWDLTAGGAQRGGPLRG
                     LLERSITPVWEANHVWLPFLLVVLWTAFPRFFGSVTSTLYIPLFGATAGIILRGTAFV
                     LRPHATSLGQARLFGAGFAVSSVLVPFCFGAAIGGIASGRVPVGNAGGDPWSSWLNPT
                     SITVGVLAVITGAYLAAIFLAGDAERAGEPELERATRRRALISGVVAGAVALGALAVV
                     RSDARELYDGLTSGAGLALVIASAILGIATLVLVWTERLTVARFTAAAAVACVIAGWA
                     AAQSPYLLPGELTLDQAAASNATLVALVIAAGAGLALVLPSLFLLYRLVLRGRLDQPY
                     QPLDMRWRS"
     gene            complement(32750..34150)
                     /locus_tag="VF752_12800"
     CDS             complement(32750..34150)
                     /locus_tag="VF752_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX8856468.1"
                     /translation="MALTSVLAAAQSAAPALGPVDQQHLLWARELQAMSFAFHIPLVC
                     FGVGFPAIVLFVEGLSLRTGDPAYRALAKRWSKAMTVLFAIGVVTGTILSFELGLLWP
                     GFTGTFGDVFGVAFGIEGTSFFIEAIFIAIYVYGWERLPPKVHLLSGVPIVVTGITGS
                     LMVISVNGWMNHPVGFDVTPGGRVTNVRPWKALFNPNFWHEFVHMYFAAFMVAGFVVA
                     GIYAVSWLRGDRSRYVRAALAVPLTAAALAAPVQVLVGDWNGRTVANYQPTKLAAFEG
                     LYHSEHGAPIHVGGIYHDNKVKFGIPLPNLLSVLAKHDPNARIQGLEEVPPQDRPPVN
                     TVRYAFQLMVGIGTLLAVLGVVFVLTWWRHGRLPRTPWFYRAVVVAGPAAVVALICGW
                     VTTEVGRQPWIVYDVMRTKQAVTNAGGLPVVFFGMLIVYLGLAAVAVWMTRRLAARPP
                     EAELSDTEGAPWPKPA"
     gene            34240..>35403
                     /gene="radA"
                     /locus_tag="VF752_12805"
     CDS             34240..>35403
                     /gene="radA"
                     /locus_tag="VF752_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX8856469.1"
                     /translation="MAKTVFVCSSCAAESAKWHGRCPSCGEWNTMVEERAAAPPRGAP
                     SGGGAKRAARALKPVLLSEVEAPHVERFSTGIAELDRVLGGGIVPGSLVLIGGSPGIG
                     KSTLTSMALGNLQQAGKRTLYVSGEESAAQIRLRAERLPGAALEVPTLAETDLDTVLA
                     TLDAEQPEVCVIDSVQTLHAADLTGAAGSVGQVREVADRITRLAKARDIAVLLVGHVT
                     KEGALAGPRVLEHLVDCVLQFEGERERTYRTLRALKNRFGSTNDVGVFEMRQGGLVEV
                     EDASARFVGEATRAPGSAVLAAMEGSRPLLVEVQALVAPTEVVPPRRVATGIDRNRLA
                     MVIAVLARHGGPSLASADVFVNVAGGVRVDEPGADLAVALALASAHRGETLADA"
BASE COUNT         5585 a        12020 c        12438 g         5360 t
ORIGIN      
        1 gagttgcgcc ggcgcgcggt gctgccgctg ctcgtccttc actacatcca ggcgggcccg
       61 tcctacggca accagctgat ggagcgcatc tccgacgcca ccgcgggcgt gctctcggtg
      121 aatccgaaca cgatgtaccc gctgctgcgc cagctcgagg cgcggaacct gatcaggggc
      181 gaatgggagc accccgagcg ccgaagccgc cgctactact cgatcaccga cgagggccgc
      241 gcggagtacc agcggctcga gaccgaggtg cgaccgttcc tcgagtccgt cgcgcgttcg
      301 atcgacgcga tcgtgagcga ggtgtacggc gagcggtgag catcgcgcgg gctgacggcc
      361 cggtagaggt gcccgcggtg accgccgcgg cgctgtggac gaacccggac cgatgggcca
      421 cgttcgtgga gggcttcgcg cacgtgaacg agaagcgcga cggctggccg gagccgggcg
      481 gcaagatcgt ctggcgctcg atccccggcg gtcggggcat cgtgaccgag cgtgtgctcg
      541 agcacacgcg cgacgacgac ggctccaccc gcatcgtctc gcgcgtgttc gaggaagcgc
      601 tcacaggcac ccagaccgcc acgttcaccc agaccgacgt gggctgcgag atggcgctcg
      661 agctcgaata cgagctggcg ccgaacaccg tgatgcgcac cggcgtgctc ggaaagatca
      721 cgaacgcgct cttcatccgg cgggcgctgt cggactcgct ggcgcggacg atcagacgct
      781 tcacgacgga ggctcacgaa gaggcatctc tgtagccttc cgcgggccgc cagaccggaa
      841 gggactcatc taaatgttcg ttttcaaagc cgccgtcgtg ggcgccggca cgatgggggg
      901 cgagatcgcc caaaccatcg caagctcggg catcgccgtg gtgctgaagg acgtcgagca
      961 gaagttcgtg gaccagggcc tcgagaaggc ccgccaggtc acgcagggcc agctcggcgg
     1021 gctcgtcaac aaggagaaga tcacccagga gcaggccgat gcccagctcg aggagatcgt
     1081 cggccgcgtc accggcacga cggactacga gggcttcggc gacgtggact tcgtgatcga
     1141 ggccgtgccc gagcgcatgc cgatcaagca gtcggtgttc gccgacctcg acgccgtcac
     1201 gcccggccac gcgatcctca gctcgaacac gtcctcgctc tcgatcaccg agatcggcga
     1261 ggccacgagt cgccccgaca aggtcgtggg cttccactac ttctaccccg cttcgatgat
     1321 gcgtctgatc gaggtgatcg agggcgacga gacgtccgag gagacgctcg gcgccgcgcg
     1381 caacttcgcg atgcagatcg gcaagacgcc gatcgtctgc ggcgaggtcc cgggcttcgt
     1441 cgtcaatcgc atcctcaact ccgcggtcgc cgaggtgtgg cgcgtgcagg agggggaggg
     1501 gatcgactac aaggagatcg accgcgtcgt gcaggagtcg aagaccgtgc cgatggggcc
     1561 gttcttcctc accgacctgc tcgggctcga caccgtccta catgtggccg agcacctcaa
     1621 ggagagctac ggcgaccgct actacgtcca ctccggcatg aaggagctgg tcgagcaagg
     1681 caacctcggc gccaagaccg gaaagggctt ctatgagcac ggcagctgat cagaccgtta
     1741 cagggacgct cgtcgagcgc ttcggcctga aggcgctggt ggaggcctgc ctcgtgctcg
     1801 aggagggcgt cgcgtccctg aaggacatcg agattgggat gatgatgggc gcgggcatcc
     1861 tgcccggacc gttcgcccgc gccgacgagc agggcctcga cgaggtgctt gcggcgctcg
     1921 agcgcgccga gcgcgactgg ggcgagtact tcgcgccgcc gctgatcctg cgccgcctgg
     1981 tgaaccaggg acggctcggc aagaagacgg gccagggctt cttcccctac gcgcagccgg
     2041 acgagggcca gtcgcgtgag acgatcgcgc tcgagacgcg cggcgacatc gcgatcgtgt
     2101 ggctcaaccg gccgcccgcc aacccgctct cgccgcaagt cgtgcgcgag ctggccgagc
     2161 tctggagcga gctcgacggc aacgcccgcg ccgtggtgtt cgcctcgtcg aacatcttca
     2221 cgttctccgc gggcgccgac atcaaggagt tcaccaggat gtcgccagac aagaaggggc
     2281 gcgagctgct cgagtccgca cacggcttca tgcgtgcgat ggagcgctcg cgcaccgtga
     2341 cgatcgcggc cgtgaactcc ctggcgctgg gcggtggttg cgagctggcg atggcatgcg
     2401 acttccgcct cgccgcggaa tcggcgacct tcggtcagcc ggagatcaac ctcgggatca
     2461 tccccggctt cggcggcacg cagcggctgc cgcggcttgt tggtgagggc aaggcgctcg
     2521 agatgaacct cacgggcacg ccgatcggcg cctacgaggc gtaccgggcc gggctcgcca
     2581 accaggttgt gccggatcac gagctgttcg acacggcgct gatgtgggcg cgcaagatgt
     2641 ccgagcaggc gccgatcgcg atctcgaaga tcaagacggt cgcagcccag ggcgacctcg
     2701 acgagggcct ccgactcgag ggtgaggcct tcggagaggc gttcggctcg gaggacgcga
     2761 aggagggcat cggcgccttc ctcggcaagc ggcggccgag attccagggc aagtgacgac
     2821 cgagaccggc gcatcggccc gcgacgctgc gagggtggcg gagctgctca cgcggtcgca
     2881 ccgcgctgtg gtgctcaccg gggccggtgt gtccgtgccg tccgggatcc cggacttccg
     2941 cacccccggc aaggggttgt gggagaacgt cgacccgatg gaggttgcgc acatcgacgt
     3001 gttccgccgc gaccccgacc gcttctggca cttctacggc agccgtttcg cgtcgctcgg
     3061 cgacaagcgg ccgaatcgcg cgcacgagat catcgccgag ctcgaggagc gcgggcttgt
     3121 ccgtgccgtg atcacgcaga acatcgatcg gctccatcgc cgcgccggtt ccgagaccgt
     3181 gatcgaggtg cacggctcaa tcgaggtctg cgtctgcccg gaatgcggcg cgaaggtcgg
     3241 catcgaccgg gtgctcgagc agctcgaggc cggcgtcggg acgccggagt gtgaggtctg
     3301 catccggccg ctcaagccag acgtcgtgct gttcggcgag ctgctgccgg agcgcgcgat
     3361 ggccgaggcc tacgcgctcg cgagcgaggc cgacctcatg ctctgcatcg gcagctcgct
     3421 cgaggtctat ccggtcgcct cgctgcctac cgtcacccgc gacgcaggcg gcaagctcgc
     3481 catcatcacc caggggtcca cgccatatga ccgcgaggcc gatgcgaaac tcgagggcga
     3541 cgtggtcgac gagctcgagg acgtgctgga ggcactcgac ctctgacccc actgctcgtc
     3601 gcgctcggcg gcgctgccgg ggtgctcgcg cgctacggcc tcggcaccgc ggtgccgaac
     3661 gacgcccgcc cctggctcac ggtcgcgatc aacgtgagcg gctccttcct cctcggcctg
     3721 ctcgtcaccg tgggtgactg gttcagcgcc gaggtgcgca ccgctctcgc cgtgggtgta
     3781 ctcggtgggt tcaccacgtt cagcacgttc tcggtcgacg tgttcaccga catcgaatcc
     3841 gggcgcggtg gcgaggcgct gctctacctg gggctgtcgg tggtcctcgg agtggccgcc
     3901 gcggcggccg gttactacgc cggtcggggg ctggcgcatt gagacaatcc gtccaacctg
     3961 ttgcgattcg cactaggcgg ccttacactg gcgccacgga ttcgcccaga cgctgcaaag
     4021 cggaggtgcg gatcccgtcc tcgggggagg gatggccggt ccgcggggct actcgggctc
     4081 ccgcggaccg gcaagctttt tggtggctgc aaacctgcgc ctggctgcgt cctcgggcac
     4141 tcgccgtatt ggcatacggc ttcggccctg cgtccttgcc atccgcggtt ttcgcaacca
     4201 aaaagcttgc cgtcggcaag cactccgccg tcggcaagca actccgccgt cggcaacttt
     4261 cactgctctg cagccctgcg tgctctccct tagtgctctc ctgccggcgc ctgcgccgcc
     4321 gatccggtgg cgctgtgctt cgcggcgctt tcagtcgtag cttgctgcca ggtgcgagaa
     4381 cctggggtcg gttccgccta cttcgctccc gttcagccag acccggccgc ggccggttcc
     4441 caggctcttg cgggcttcga ttgctcgcgc gaccgggcgg tcgtcggagc ggcggccgct
     4501 gcggcgccgg gcgttctgga tcgggatggc gtcgctcatg gcgctgatgg tcccagcggc
     4561 ttccggaggt tcgtatgtac gggtgtcggg gattccgctc atctctttct ccctgttgtc
     4621 gccacgcacg gtagggagag cgtctagcgg cgatgtgaac ggtccgtgaa gcgggaggaa
     4681 agatccggcg ctctttagga gccggaggcc tgcttcgcgc gcgcccgtgc gagggccgac
     4741 tcgagcagcg tgacggtctc cgcgacgccc tcgctgagct cgctggggac gtcccccgcg
     4801 agggcggcgt tcgcggcgtc ggccacgagc ggccgccgcg ccgcgacctc ggcgcgccgg
     4861 tcgccgagct cgtccggtcc gagctcggcc agcagcgctt cgagcgccac gcgcgtctgc
     4921 agggcagcct cgcggtggcg cccggcgtcc acgtccgcac gcgcgcgcag cacgagctcc
     4981 tctgaggcga ggatgtcctc gcgcccggcc agcacagcgg cgaggcgctc gttcggcagg
     5041 agcatctccg agcgcttacg ccggcgctcg tcgcggggga cctcgtaggc gtccgtgtag
     5101 ttgccgtccg cgacctcgga gccgtcgccg tatccgacgc ggacggcgat cgcctgagcc
     5161 gcgctcacgt cgcgcgcgta cgggtcggcg gcggcggcgc ggtgcgcgcg gagcacgcgg
     5221 ttcagctcgc gcacggccaa gtcgacctcg aaatgcagcg cctcggagtc ggtcacgagc
     5281 gcgccgagcc acgaggcggc gtcggcgggc gcggggaagg ggcccgcgtg gatcatcgtc
     5341 acgcgcgcga gcggcatggc cggcgtcgcc gcatgggtca cctgcttcgg ccgccgcctg
     5401 cgcgacaggc gcggggccgg cttcggcgcg ttcaacgtgg agatcacgac cacgcgctcg
     5461 ggggccgcgc ccggcccggc ccgggtcacg tagcggccgt cgtcgggccc caggacgaag
     5521 ccgaactcga gctgtacgaa cgcgaaggag ccctgagcgg ccacggcggc gctcgcggcg
     5581 ctagtcgccg gaggtgccgg tccttctgcc gttgtggacc ggcagcgcga acttcgacgg
     5641 cttcgtcggc acgacggggt tctggatctc gcccagccgc ttcacgaggt cgtcccactg
     5701 gccgggcttc aggacggcca ttgcatcctt gccggccttc gcattggtgc cgaacagcgg
     5761 ctggaagccg acgtggatgt ggtcgtaatg gtcgggcagc gccagtgtgt tctgcccgaa
     5821 gtcgagcagc gagatgatct ggtgcgggcg catggtgccc tggagcatca tcagccgttt
     5881 gaccgtggtc tcggtgatgc cgcctggatc ctggtgaccg gcgatcggga tgccgttgac
     5941 ggccgcgatg tcgacggcgt tgccggagga gtgctccgac acgttgcccg acgccgtgta
     6001 gaagccgtgg ccgctcttca gcgaagtcac cgtgggccgg aggccggact ccgccaggta
     6061 ctcgagcgtc gcaagcacgc ggcggtcgat ctggccggtc cggatgtcgt tgcggccggc
     6121 ggcgtagagg tcgatgcggc cgtcggagag cacgcggcgc tcgaggagcg gcttcggcag
     6181 cagcagcacc tggccgatcg agaagttgtc gccgctcgag gagtagagcg cgttgcggcc
     6241 ggaggcgcgg tagatggcgg tggcctcgag cagcttccag ccgtcgagga tcggcttcgg
     6301 atcgatgttc ggggcgccct tgcccgccgg gcggatctgg aagtagacgt gcggcgcgag
     6361 cttcgcgtcc gtcttgccga tccggccgag gatcgtgccg ccgaccacgt gcgagccctt
     6421 gtggaggcgc cgcagcgtcg cgttcttgct gttcagcccg agcgcgcgcg agaagtagtt
     6481 acggaaggtg gtgtagccag cgcccgccga ccgcgagttg aagatctgct ccaggccgcc
     6541 atggcgcttc gcgccggcgc gactcgggtt ggcgaacagg cgctgcttcg tgatgacggt
     6601 cggcgtggcg gcgccctgac cgctcgactt gccgtgcgcg gcgaggcgct gcttcgcagc
     6661 ctgcttcctc gcgagctgct tcgagcgctt cgcgttggcc ttggcggcct gcttcttgct
     6721 cgccttcgtg acctggcggc cggcggaggc gggctgcgtg ggcttcggaa gcttctggtt
     6781 cgcgggcacc gcgttggcgc tcgtcttcgt caccttgccc gtgtccttgg gcaccgggtg
     6841 cacgcgcgcg aggctgctca ggcctgagta cgtgtactgg ttgccgtacg cgtcctggag
     6901 cgtgacgaac aggccgagct tcttcgagcg gcccatcttc ttgatcacgc cgtcgttcgt
     6961 ggcgaccacc ggcgcgcccc ggcgcgcgaa gatgttgatg cccttgcggt tcgagtcgct
     7021 gccgacgagc cgcgcggcgt tgtgcccctt cttgatcttc ttctcggcct gtgcctcggt
     7081 gatgtcgtcc gcgtagcgcg cgtgcgcgta aacagggaag cggccctcgg tgagacccgt
     7141 cagcgatccg atcacgtcgg ggggcacgcc agcgatgagc ttcgcgcgga gcatcaccga
     7201 gtcgacgtac cagtcagcgt ggttgtaggc gaagatcgcg cggcggatgt cctggtcgcc
     7261 gcccgccgcc ttgaggtacc tggccgcggc gaagattgcg tcgaccgggt tgtacgggtc
     7321 cctgcggccg tccttgttcg cgtcggtgcc gtacatcttc cagctcgagg gcatgaactg
     7381 catccagccg acggcgcccg cggaggagac gttgaggttg cggccgtagt cggtctcgat
     7441 ctcgttgatg ccggcgagga tctcccagcg gatcccgtac tggatgcctg ccgcctggta
     7501 gatcggcagg aggaagggcg ggacgcggaa cttgctgatg acgaagttgg gcacgccgct
     7561 cacctgggcc gggcccggga gcgcgtcgaa gaaggtcggg ttgtgagggg tggggacgcc
     7621 gtccggccgg cggatcggat tgcccgcggg agtcttcggg gtgttcttgt cacgctcagc
     7681 cttacgtcga gccttcagac gttcggcgcc cgagcgccgc tgacggtgtc cagctcgggt
     7741 gttcggagcg ccggggacct tcggcacctc ggcgttgggg cccgtgcccg tcacgccacc
     7801 ggcgccgccc gtcgagccgc cgccgcctgc gccgcctccg ccgccggtcg tgccgccgcc
     7861 ggtgctccca ccgccgccgc cactgcctcc gccaccgccg gggagcgttg gcacgcccgg
     7921 caccgtcggg acggtcgggg tggggagcgg aacggtcggg gtcgggacgg gggtggtatc
     7981 agtcgggatc gtgattccgt ccaggggcgt gcccgtggac ggaacggtcg gcacgggcaa
     8041 ctggctgccg gtcgcgtcct gcgcctccgc ggggaacgag aggcctgtga agacagcaag
     8101 gaccaggcct gccgaaagca ggatgtagag gaggctcttc ctcatccttg gtctctgtct
     8161 cctcccgtgc ccctctgggc ccgcttggtg agggccccgc catggccgcc gttggacgcg
     8221 cgcaagctac cagacctgcc cgctgcggca ggaacgctac ttgctgatat ctggctgtgc
     8281 aactgggaac cgttcatgga ggggtagagt cgcgcgcgta tggagggccg cgggaccctg
     8341 tgggttccta tcggccgaaa tgctccccac ctacagtcct tccaacgtcc cgccggcgtt
     8401 gaaaggtgcg cttggttcta cccattccaa tgagcaaaca agccgcaccg gtacgttcga
     8461 cctgaggttg agagttaggg ttacacgtta cgccgcatga ggtgaagcgc gtccccggct
     8521 atgcgctacg ctgccgcgcg tggccgagca cgagcacgac gagggcctgg tcgaccggct
     8581 ctcgcggcag ggtgaagagg cgctcggaaa agtggccgag cagctgctgt cgaatcccgc
     8641 ggtggccggc gccatctcgc gtgcgttcga ggctcgcgag aaggcatctc aggcccagga
     8701 ggcggcgatg ggtgcgcttg gcatcccctc cgccgccgac atcgagcggc taacccgccg
     8761 gctgcgctcg gtctcgcaac ggctcgaaag catcgaggac tccgtcgacc ggctcgacga
     8821 gcggctcgag gccatcgacc ggcgcatcgg cgaggtggcc ggcaaggcgg gttcgagcga
     8881 tgaggtggag tcgacgctct ccgacatctc ggaccggctc gaccggctgg ctcgcgacct
     8941 tgccgcgctg cgcgacgcgg tggcgccggg cagcgagcca ccgccgcgcg cgcaggagcg
     9001 cctgaccgtc agcgacggct gagcgccgcg taggccgtcc ggccgagcgc cagccccacc
     9061 tcctccagcg cctcgcggtc gatccgcacg cggccggcgg cgatctcgtc cgacaccgcg
     9121 agcacggcgc cggtcgccac accgcgcagg gcggcgagcg tgaacagcgt cgcgcactcc
     9181 atctcgacgg cgattgcccc ggcgtcgagc cagcccgcca tccggccgtc gcgcgtgtcg
     9241 tagaagaggt cggtggagac gacggcgcct tcgcggccgg ccgcctcggc gagcgagcgc
     9301 tcgagcgccg cgtccggagc tatcggcccc gtggcgccga gcgcgcggct tgcgccgtcg
     9361 tcggggagcg cggagctcac cgcgaagaca tcgcccaggg cgagcccgcc gccgaacgcc
     9421 ccgcaggtgc cgatccggac gagcgtctcc gctccgaggg cgatcagctc ctcgaccacg
     9481 atcgccgcgc tcggaccgcc catcccggtc gactgcacgg tcagcagctc gccgtcggcc
     9541 gccggcccgg tgtagcccca gagcccgcgg tgatggttga acatcttcgg gctctcgagc
     9601 aggtcggtag cgaccgcgag ggcgcggtgt ggatcgccgg gcagcagcac ccgcggcgcc
     9661 agctcggcgt ttggcttcag atgaatcggc tcgggcgcgg acgcggcggg cacgcggctg
     9721 acgatatgcg agactccgcg ctcgatggcc aagcggccca cacagccggt tgaggaggct
     9781 ctgcgcgatg ccgtggagcg caccgtgcag gccacgatcg gctctgcgca ggactctcgc
     9841 acgcgcgctc agcaggcggt ggacgagatc gtgaagagcg ccgaggccgg ggcgaccgct
     9901 gtccgcgagc gcgtgcgcgg cgcgttcgag ggccagctgc cggccacgca cgacgacgtg
     9961 cgcgaactcc agaaggagct acgcgcgatc gtcaggcgcc tcgacgagat cgagaagcgc
    10021 cttccgaagc agcgatcttc gtcgggcacc cgcaagaaga gctgatgggc cgccgggtgc
    10081 tgatcaccgg cgtcggcagc ttcctcggca cggagctcgc ccgccggctg gagaccgacc
    10141 cggagttcga cttcgtcgcc ggagtggacg accgcgcgcc gcgcgcccgg ctcgagcgca
    10201 ccgagttcgt cgaggccgac atccgcagcc cgatcatctc gcggatcgtg ccgcagctcg
    10261 gcgtcgacac gatcgtgcac aacaagatcg tgcggcgacc ggggcccacg ctttcggctc
    10321 gcgccgcgca cgacatcaac gtgatcggga cgctgcagct gctgaccgcc tgcgagaagg
    10381 ccgacacgct cagctcgatc gtgattcgcg gctcggccgg catatacggc tcggagccgg
    10441 cggcgccgca gttcttcacc gaggacatga gccggctgta tccgctgcgc acgcgctttc
    10501 agcgcgacgt cgccgagatc gagaactact tcgagacctt ctcgcgccgg caccccgcga
    10561 tcacctgcac gatgctccgc ttcgagccgg cgatcgggcc gacgctcgac acgcagatca
    10621 cgcgctacct atcgctgccg atcgtgccga cctacttcgg cttcgacccg cgcctccagt
    10681 tcgtgcacga ggtcgacgcc atcgaggcca ccgtggcggc gatcaagcgc ccggtgcggg
    10741 gcgccgtgaa cgtggccggc gccggcacgg tcgggctcac aaagctcgtg cgcgcggcgg
    10801 gcaagctgac gctgccgatc gtgtcgccgc tcttcggagg tgtgagcgcc gtggccaaac
    10861 ggctcggact gctcgacttc tccgacgact tcgtacgcct cctccactac gggcgcgcgg
    10921 tggacatcac gcgcctcacc gaggaggtcg gcttcacacc cgcctattcc acgcccgacg
    10981 ccgttgagga ctacgtacgg acgctcggtg gccggcgtct cgcgccgccg cttcgccagg
    11041 tggtgtcgct gtgagcacgg agacgcgcgg cccgcggacg cgttcgaagc gggcggctcc
    11101 cgacggcggc gtctcgccag gcaccgacca gtcgcgtgtc ggctctagcg gcgccccgcg
    11161 cggctcgttc gccttcggcc ccgcgatcgg cttcctgcgt aggttgcgcg gcgcgctcgg
    11221 gccgagagtc gatctcccgc aggcgttcgc cgaggcgacg ctggccctcc cgcgcgaggt
    11281 gcgcgaggcg atcgacgccg gccgaaggcg gctcgcgggt gactaccagg aggacgagtg
    11341 gggcttcgac gaggagttcg ccgaagcagt ccgcccgttc ttcgacttcc tctacgacgc
    11401 gtggtggcgc gtcgaggcga agggggtgag caacgtgccg tcgcacggcc gagccctgct
    11461 cgcctccaac cacgccggca tcctgccctg ggacgcgacg atgatctcga ccgcgatcct
    11521 gcgcgagcac ccgctgccgc gctacccgcg cttcctggtg ctgaactggg cgttcgagct
    11581 gccgtacgtg tcggtgctga tccgcaaggt cggcggcgtg gtcgcctcgc cgtacaacgc
    11641 gatgcggctg ctcgagcagg acgaactcgt cgccgtcttc cccgagggcg tgaagggcac
    11701 gggcaagcac ttccgcgacc gctatcggct acagcgcttc ggccgtggcg ggtttgtcga
    11761 gatcgcgctt cgcaccggct cgccgatcgt gccggtggct gtcgtcggca gcgaggagat
    11821 ctatcccaag atcggcgagg ccccgctgct cgcacgcgca gtaggtgcgc cgttcttccc
    11881 gatcacgccc acgttccctg cgcttggtcc gctcggcgtg gtgccactgc cgtcgaagtg
    11941 gcggatcgag ttctgcgacc cgatcgagac ggcgcactac ggccctgatg cggccgagaa
    12001 ccgctcgctc gtcttcgagc tgtcagagcg ggtgcgcgac acgattcagc agacgctcta
    12061 cgagaatctc gtggagcgcg gttccgcctt cctatagacg cgcgcacgcg agccgggtga
    12121 caatgacggt gtcgtgcggg catccgacga cacccgggag cgagcggcga ggcgtcttcg
    12181 cgaagggtgc gcggacggcc gcatcagcgt cgacacgctg agcgcgaggc tcgaccggct
    12241 gttcgccgca cgcgaccgtg gcgaggtgca ggccgtcgtg gccgacttac cgaggcggcg
    12301 cacgctcgct tcgctgttcc gccgtgctca gcgtctcgtg ccgccactcg tgccggacgg
    12361 ggagaccctg acgctcggcc gcggcagcga ttgctcgctg ccgctcgacg acccgttcgt
    12421 atcgcggcgc cacgcggagc tgtcgcgcag cgaccgaggc tggctgctgc gcgatctcgg
    12481 ctcgaccaac ggcgtgtggg tgaacggctg gcgggtgcgc gagagcgaac tccgcgacgg
    12541 tgacgaggtg cggctcggct acacccgctt ccgctttggg cccgggtctc gctgaggagc
    12601 gcgaacttgc gcacgccgcg gcgcgcgagg ttagggttgg cccgtgggct tcaagtctcc
    12661 ggctgagttc cgggaagtga tggatcaggt gttcacgatc atgagcaccg actccgagat
    12721 gggcccgaag ctgcgcgacg cacggacgcc gcagcgtttc gagttccccg atctcgacct
    12781 cgtcgtgaac atcacctacg tcgaggacgc cgaggccgcc gcgaacggcc agaacctgcg
    12841 ctgggagtgg tcggacaacg tggactggga gcccgaggtc cagatgacga tggactcgga
    12901 cgtcgcgaac cgctacttcc agggcaagga gaacatcgcg atggccatcg cccgccggcg
    12961 gatcaagacg tcggggaacg tgaagaaggc gctgaacctg atcccgatca ccaagcccgt
    13021 gttcgagcgc taccgggcga tgttggccaa ggatcacccg gacttgctcg agtagttcct
    13081 ggtacggcct tggaggaggg cattcccctt cgggctccgt tcgcgcttcg cgctcactct
    13141 tgacgccctt cggggaacgc cctgcctcca ggcctactcg agcgttcccg gcggccgggc
    13201 tcccactcgt tagtgctgct ttccagaaga gccaaggcga cgcggggtgg aaacagcagc
    13261 actgatagat ggcgagcgtg gcgttgcgaa gcgctgccgc acgacgccgc ggcgatccgg
    13321 gacagcagca ctgacggatg gggacgcggg ctggcgccca ggccgagcgt cgtcattttg
    13381 ggcccatagt gggcccgtaa tgacgacacc cgctcttgcc aacgccgccc gtcgtcagtt
    13441 agggggaata gtcgccccta agtgacgacg gaccgccgcg agcccacgcg cggcgcacta
    13501 cggccggccc gagtaggccg ggaggcaggg cgttccccga agggcgtcaa gagtgaggcg
    13561 aagccgaacg gagcccgaag gggaatgccc tcctccaagg ccgtacaaga aacgaacgcg
    13621 ccctagaagt gaactccgcg aaggagatag gccatcgcca ctgcctccgt cgacatctcg
    13681 ccgtccttgg gcttctcgcc gtccgagcca gccccggccg ccgccttttc cttatcgccc
    13741 ttcgcagcct tcgagtccgg gaagatcttg tacgcggtgt tcatgatctg gtcgacgacc
    13801 ttcggcgaca gcgcgtagag cacctggccg aacgtcccaa ggcgcgtggc cacgcgcttg
    13861 ggcttgtcga tgatcgcgtc ggcgatcagc tgcgcggcct cgtccggcga cagcgtcggg
    13921 aacgcgtcgt aaatcgaggt gggcgcgatc atcggcgtgc ggacgagcgg catgtagacc
    13981 gtcgtgacgc tcacgttgtc gccaacgacc tcgggcgcca tgcagcgcga gaacgcgtcg
    14041 agcgccgcct tcgacgccac gtaggccgag aagcgcggcg tgttcgtctg caccccgatc
    14101 gacgagacgt tgatgatgtg gcccttcttg cgctcgcgca tcccgggcag gaaggccatg
    14161 atcagcttca gcgcgccgaa gtagttcagc tgcatagtgc gctcgaagtc gtggaagcgg
    14221 tcgtacgagg ccgcgatcga gcgccggatg gagcggccgg cgttgttgat gagcacgtcg
    14281 acgccgccgt actcgtcgag gatctcctgc gccacccgct cgatgtcttc gaggtccgag
    14341 aggtcggctg ggtggacgtg ggcgatgccg ccggcctcct cgacttcggc cgccaccgcg
    14401 tcgagcttct cgcgcgtgcg cgagatcagg atcacctcgc cgcccgcctc gccgatcttg
    14461 cgcgcggtct cggcgccgat gccggaggag gcgccggtga tgacgatctt cttgccctcg
    14521 accgacttcg acagcgagcg ctcgcggaag agatccgggt cgaggttgcg ctcccagtag
    14581 tcccagacct tcggcgcgta cgtggacagc ggcggcacgg cgatgccggt gcccttgagc
    14641 gcgcgctgcg tgtcgcgcgc gtcgaagtcg cagcggaagt cgcggtactc gagcacgacc
    14701 ggcggtatgc cgagatcgtc gagcacctcg tcgcggatgc ccttgatcgt gggcaccgcc
    14761 atgagcccgg ctcgcacctg cttgggcagg acgttcgtga ggttctggtc gacgcgcatc
    14821 gcgaagcgcg gcgcgtgcgc ggccttcgcg aactcgttca gcacctggcc ggccgacagc
    14881 ggcttcgggt caacgaggtg gaacgtgttg cccggcaggt cgtcgtcttc ctggtgggcg
    14941 atgtggtcga gcgccttcgc cacgaagtcg acgggaacga tgttcgcctt cttgccctcc
    15001 gggccggcca gcgggaacca ctcgggcaga gcgtggcgga gcttctggat tagcttgaag
    15061 aagtagtagg ggccgtcgat cttgtcgatc tcgcccgtct ccgagtgccc gatcacgatg
    15121 cccggccggt acacgcggaa cggcgcctcg acgtgctctc gtacgagctt ctcggactcg
    15181 aacttggtct ggaagtacgc gtgcgggagc ggctgaccct cgtcgaacat gtcctcgcgg
    15241 aacaggccct tgtagcggcc cgcgaccgcg atcgagctca cgtggtgaaa gcgcccgagg
    15301 tcgatcgagt tcgcgaactg gattgtgttg cgcgtgccct caacgttggc gcgctgcatc
    15361 gactcgtcgt cggcctccat gtcgtagacg gcggccaggt ggaagaagtg gtcgatcttc
    15421 tcgtcgaagc tttccatgcc gagcttctcc ttggagaggt cgccgacgat gggcacgatc
    15481 cggtcttcgg caccaaggct cttgcgcagg tcgtcgaggc gcccacggga gccctcgcgg
    15541 acgagcgcgt aaacggtccc gtcacgcttg agaagctcct gcaccagatg ccggccgatg
    15601 aagccggtgg cgccagtgac gaaatagttc acgctttccc cctcagtgga tctccttctc
    15661 cgggcgcgag tctatagagc gtgagtgctc atccgcgacc gggtagcgac cgcctacgga
    15721 cgcggtattg acggatccga cgagcggcgg aacacttcgt gcagcccact aggcagcagg
    15781 ccggaaagcg ggaactcaca gccccgctag gcgcgcagcg ccgccggtcg acgtcgcggg
    15841 acgttaagcc cgcgtgcggc ggggccgtcg cttacggccc cgccgttgta gcttcagcga
    15901 gcgtatgacc gccgaggccg cgaacgccag atccactccc gcgccgagcg gcggtctgac
    15961 gttgcgcgag gtggccgaag aagtcggcgt gacgcccgcg acgctcaggc ggtgggtcaa
    16021 ccagggggtc gtccctggcg agaccgatgg gaggccgtgg ccgcccgccg cggttgcgca
    16081 cgcgcgcatc gtcgcgcggc tcagggagcg cggtcactcg ctccagaaga tcaaggaggc
    16141 gaacgacgag ggccgcctcg ccttcggctt cctcgaggac ctgttcccga cgccgaagcg
    16201 gcggcggaca ctgagggagg tggcgaagga gaccgacctc gagcccgagc tgatcaagcg
    16261 catctggggc ggcatcggcc tgccgcgacc ggagtcggac gacgacccgg agttcaccga
    16321 cgaggacgta caggcgcttc agtacgtgtc cgccgtgctc gcctcgggct tcccgctcgt
    16381 cgcgttcctc cagctcgtgc gcgtgtacgg ccaggcggtc tcgcagatgg ccgatgccga
    16441 gacccggctg ttccacctct acgtgcacga gccgctgatg cgcgagggcg tccccggctt
    16501 ggagatggcc gaggagatgg agcacctcgc gcgcgacctg ctgccgctct cgtcgccgat
    16561 catggacttc atccaccgcc gcttcctcca gcacttcgtg gagcaggacg tgatcggcca
    16621 catggaggtg gacttcgacg acgagtcgga gctcggccgc gtgcgggtgg tgatcgcgtt
    16681 ctgcgacctc gccggctaca cccgctacac cgaggagatg ggcgaggagg aggcgctcag
    16741 ctacgtggag cgcttcatcg acgccgtcac cgagacgctc cccgaggacg cgcgcgtggt
    16801 gaagacgatc ggcgacgagg tgatgatcgt cggctccgac gtcccggcgc tcgtggactg
    16861 ggcggtcggc ttccagcgcc tgttcgacga ccgccccgcg ccgcgcatcg gcatccacac
    16921 cggcgccgcg atctaccgcg acggcgacta cttcggccgc gaggtgaacc tcgccgcgcg
    16981 cgttgttgcg cgtgcacgtg gcggcgaggt gctgatcacc gacaccgtgc aggagctcgt
    17041 caaggacagc ccgcatctca cgttcgagcc agtgggagag gtgaagctga agggcttcga
    17101 cgagccgcgc agtctctgcc gcgcgcactc ggcggagtga gcgcgctcga tgccgcccgc
    17161 gagagcgggc tgatcgtggc gggcgagccc ttgctcgtga tgctctcggg cggtgccgac
    17221 tcggtctgcc tgctcgacgt ggcgcgcgag ctgggcgcgg acgtctcggc gctacacgtg
    17281 aaccacgggc tgcggccgga ggcggatgac gacgaggcgt tctgcgtcga gctctccggc
    17341 cggctcggcg taccgctcgc cgtcgagcag gcccggcccc cgggccgctc caacctccag
    17401 gcggaggcgc gcgacctgcg ctacgcgctc gccgagcgcc atcgcggccg tggcgactac
    17461 gccgccgcgc acacggcgac cgatcaggcc gagacagtgc tctaccggct cgcggtctcg
    17521 cccgggcggc gggcgctgct gggcatgccg gcgcgccgcg gccatctcgt gcgcccgctc
    17581 ctcgaggcca cgcgcgccga gacgcacgcc tggtgcgagc agcgcgggct cgcctggcgc
    17641 gaggaccgct cgaacgtcga tccgcgcttc gcgcgcgcgc gggtacgtag agaggtgctg
    17701 gcggttctca gcgagctgaa ccccggcgcc gagcgcaaca tcgccgagac ggcggacctc
    17761 ctccgcgacg aatccgaggt gctcgacacg gccgccgacg aagcgctggc ccgtatcggc
    17821 ggcgaggccg ccgtgccgct cgcggaactg cgcgcgcttc cccgcgcgct cgcgcggctc
    17881 atgctgaggc ggatggccga gcgcgccgcc ggcggacagg tggcgctctc gaggcgcgac
    17941 gcggacgccg ttctccacct cggcgaccgc ggcggcaccc gttcgctcga cctcggcgga
    18001 ggcctccgcg ccgtggccga gtacggcacg ctcagattca cgcgcgaggc acccgaaggc
    18061 gaacagccgc cgccggagcc cgtactgctc accgttccgg gaagcgcccg attcggcccg
    18121 tgggaggtgg tggcagagct gggtccgggc ggcgaggccg agcttgccgg cgccgagctc
    18181 ggcacggccg tgaccgtgcg cgcctggcgc gagggcgacc gcatgcgccc agcgggcctc
    18241 gggggctcga agagcctcca ggacctgttc accgaccgca aggttccgcg cgggatgcgc
    18301 cggtcgctgc cggtcgtcga ggccgaggga gagatcgcgt gggtggcagg cgtcgcgatc
    18361 ggtgagcgct tccgtgctgt gcccaacgcg gacgaggtgg tcagcctgtc ggcccggccc
    18421 gccggaacat agactcgtga cggtgaacaa ctcttcgatc ggcgagacgc tggttgctga
    18481 ggacgatctc agacgccgca ttcgagagct cgggcaggag atcaccaagg actaccaggg
    18541 gcgcgacctc atgctggtct gcgtgctcaa aggcgcggtg ttcttccttt ccgacctgat
    18601 gcgcgagatc gacctgccgt gcgaggtcga cttcatggcg gtggcgtcct acggctcctc
    18661 gaccgactca tccggcgtgg tccgcatcct gaaggacctc gacaccccgc tcgacggccg
    18721 cgacgttctg atcgtcgagg acatcgtgga ctccgggctc acgctctcgt acctcttgcg
    18781 catgctgaac gcgcgcaagc cggcgtcgct cgaggtgtgc gccctgctca ccaagcccga
    18841 acggcgcgag gtcgacctgc cgatccgcta cgtgggcttc gagatcccga acaggttcgc
    18901 catcggatac gggctcgatc acgtcgagcg ctatcgcaac ctgccgtacg tcgcgagcct
    18961 cgctgatccg gcctagttcg gctttcgtaa gcggtcgtac gggcgctgtc aggcttcaat
    19021 ccagcccggg tatcgctgct acgcttgatt tccccctctt cttcgacggg gcgtaccacc
    19081 gaaagagagt tccgtgagcc ggttcttcaa gagcgccgcc ttcccgattc tgatcgtggt
    19141 agtgctggcc ttcttcgccc agcggctgat cagcccgggc gaccacacgc ccacgccgac
    19201 ctactcggac ttcctgaccc aggtggatca gaaccaggtc aaggacgtca cggtcaacac
    19261 gaaggacaac acgatcgacg tgacgccggt cggcccgaac gcgaagaagt actccacggg
    19321 ctacccggac aacacggaac cgcaaatcac caacctgctg cgcgagcaca aggtcccgat
    19381 cgacgtgaag gggaagggat cgtccggctg gctctcggtg ctcacctacg tcttaccctt
    19441 cgtgatcttc ctgctcttct ggctattcat catcaaccag atgcagggcg gggggtcgaa
    19501 ggtcatgtcc ttcggcaaat cgcgcgccaa gcgcatgtcg gtcgactcgc cgaagatcac
    19561 cttccgcgac gtcgccggcg tggacgaggc ggtggaggag ctccacgaga tcaaggagtt
    19621 cctcgagaac ccgaagaagt tccaggcgct cggagctcgc atccccaagg gcgtgctgct
    19681 ctacgggccg cccggcaccg gtaagacgct gctcgcccgc gcggtcgcgg gtgaggccgg
    19741 cgtgccgttc ttctcgatct ccggctcgga cttcgtcgag atgttcgtcg gcgtgggcgc
    19801 ctcgcgtgtg cgcgacctgt tcgagcaggc caagcagaac agcccctgca tcatcttcat
    19861 ggacgagatc gacgccgtcg gccgccatcg cggcgccggc ctgggcggcg gtcacgacga
    19921 gcgcgagcag acgctgaacc agctgctcgt cgagatggac ggcttcgaga tgaaggacaa
    19981 catcatcttg atcgccgcca cgaaccgtcc ggacatcctc gacccggcgc tgctgcgccc
    20041 gggccgcttc gaccgccaga tcgtggtcga ccgcccggac cgcaagggcc gccagaagat
    20101 cctcgaggtc cacacgcgcg gcaagccgct cgccaaggag atcgacctcg acaacctcgc
    20161 cgcgcagacc cccggcttca ccggcgccga cctcgcgaac ctcgtgaacg aggccgcgct
    20221 gctcgccgcg cgccagggct cgcgcgagat cacgcatgtc cacctcgagg aggggatcat
    20281 gcgcgtgatc gcgggcccgg agaagaagac ccgcgtgatg agcgagaagg agcgcaagat
    20341 caccgcgttc cacgagatgg gccacgcgat cgtcgcccac ttcctcgaga acacggaccc
    20401 cgtgcacaag atctccgtga tctcgcgcgg gcaggcgctc ggctacacga tcagcctgcc
    20461 gaccgaggac aagttcctca cgacgcgcgc cgagctgtcg gacacgatgg cgatgacgct
    20521 cggtggccgc gccgccgagg agattgtgtt cggcgaggtc acaaccggcg cttcgaacga
    20581 cctcgagaag gtcaccgcga ccgccaagca gatggtcatg cgctttggca tgagcgagaa
    20641 gctcggcccg cgggtgttcg gccacgacca cgggcagccc ttcctcggcc gcgagttcag
    20701 cgccgagccg gactactcgg acgacgtggc gcgcgagatc gacgacgaga tccggcgcat
    20761 cgtggaggag gcccaccagg tcgcccgcga catcctcatg cgctatcgca agcagctcga
    20821 caacgtgtcg gacatactga tgcgccgcga gacgatcgac cgggaccagt tcctgcagct
    20881 gctcgacggc aagaccgagg acgacgtctt cggtgccgag gagcccgagc tgccggagcc
    20941 cacgccgccc gcgcccgaga ctccgaagcg caagccgggc atcgagccgc ggccgctgcc
    21001 gcgcccgggc ctcgccggag ccgagatgcg ctctgacgag cccaagaagc ccgagatctc
    21061 ctagcgacgt aggctgcgcg ccatgagcgc gcatacccgg ccactcgtga tgggcgtggt
    21121 gaacgtcacg ccggactcgt tctcggacgg cgggatgttc ctcgacgccg accgcgcgat
    21181 ctcgcacggc cgcctgctgg cgtccgaggg cgccgactac ctcgacgtgg gcggtgagtc
    21241 gacgcggccc ggcgccaagc cggtcagcga gcccgaggag ctgcggcgcg tgctgccggt
    21301 catcgagcag ctcgtggacg agggggcgcc gccgatctcg atcgacacga tgaagtccag
    21361 agtggcgcgt accgcgctcg aggcgggcgc gctcgtggtg aacgacgtgt cggccttccg
    21421 tctcgatccc gagatggcct cggtcgtggc cgaggccggc gtggactgct gcctcatgca
    21481 catgctcggc gagccgcgca cgatgcagga ggatccgcgc tacgacgacg tggtgtcgga
    21541 ggtgaaggcc ttcctcgagg agcggctcgc gttcgcactc tcgcagggaa tcgcggagga
    21601 cagcgtgtgg ctcgaccccg gtatcggctt cgggaagacc gtcgaccaca acctcgagtt
    21661 gctgcgtcgg ctcgacgaga tcgcggcgat cggccggccg ctcgtgatcg gcacctcgcg
    21721 caagagcttc ctcggcaaga tcaccgggcg cgacgtgggc gagcggctcg ccggcacgat
    21781 cgccaccaac gtgctcgcct acgagaaggg ggcgcgcatc ttccgggtgc atgacgtggc
    21841 gcccatcgtg gatgcccttg aggtggccgc tgctacggtc cgcgggaagt ggacgacgag
    21901 cgcgagcacg aggacctaga gcccgacgag gacgaggacg agggggagga cgagctcgag
    21961 cctgcggtat cggtcgagat cacgggcctc tccgtctaca cgcaccacgg cgtcgaacag
    22021 gccgagcgcg acgtggggca gcggctcgtg ttcgacatct cgttcgagct cgaccactgc
    22081 gacgcgaccg ttacggatcg gatcgaggac acggtcgact acgccgacgt gtgccagcag
    22141 gtggcgctcg cggcgcaggc ccgctcctac gcgacgctcg agcggctctg ctcggcgatc
    22201 tgcgaccggc tgctcgaccg ctacgagacc gagtgggtca ccgtgcgtgc gaccaagccc
    22261 gagccgccga tcccgctgcc ggtggatgag gtctcggtag aggtctcgaa gcaggcgggc
    22321 tgaccgggtt gcgtccgagc tcgcgcgccg gccggacctt gagttcggct tccttctgca
    22381 cgacgtcggc aaggtcggcg tacctgacgc gatcctgcac aagcccggcg cgctcacgga
    22441 gccggagatg aggatgatgc gccggcaccc gctgatcggc ttcgagatcg tgtcccacat
    22501 cgacttcctg cacggcgcgg cgcgccaggt ggtgcgctcg caccacgagc gctgggacgg
    22561 cgcgggctac cccgatcgca tcgcaggccc ggaaataccg ctggccgcgc gcgtgttctc
    22621 cgtggccgac acgctcgacg cgatcaccac cgaccgcccc taccgccagg gtgcctcgat
    22681 ggacgaggcc cgcgagcggg tctccgccga ggcggggcgg cagttcgacc cgctcgccgt
    22741 cgaggcgctc ggccaggtgg acgagcgcga tctggaggag atcgcggcgc tgtgagggcc
    22801 tacctcggcc tcggctcgaa cgtcggggac cggttggaca acctccgggc cgcggtggcc
    22861 gcgctcgaag cgcggggcat ccacgtggtc ggcggctcgt ccgtctacga gaccgagcct
    22921 cagggcgagg tgagggacca gccggacttc ctcaacgcgg ccgtcgcggt cgagacggaa
    22981 ctcgggcccg aagagctgct cgacgagtgc aaggcggtgg aggcgaacct cgggcgcgag
    23041 ccgggcggtg tgcgccacgg gccgcgcgcc atcgacgtgg acctcttgct gctcggcggg
    23101 ctcgagcacc gctcggagcg gctcacgctg ccgcaccgcg acgtgctggc gcggcgcttc
    23161 gtgctcgtac ccctgctgga gctcgacccg gaactcgcgc ttccaaacgg gacgcggctc
    23221 gcggacgccc tctccgcact cgaaggccag cgcgtggagc gcgtcggccg cctctgagcg
    23281 agcccaacct ctgagcgagc cataatccgc gcgcgcatgc tgcttgcgat cgacaacggc
    23341 aacacccaaa cccacgtggg catgttccgc gacgggcagc tcgtcgagca ctggcggctc
    23401 gcgaccgtgc gtgagtcgac ggcagacgag ctggcggcct ggctctccgg cctgctcaac
    23461 ctacgcgggc tgtcgctcga cgacgtggac ggcgcgatcg tgtcgtcggt ggtgccgcag
    23521 ctcgagcacg agtacgggct cgtgtcggag cgctacctga agggctcgct gatagtgatc
    23581 gggccgaaga tcaagaccgg catgccgatc cggatggaca acccgcacga gctcggcgcc
    23641 gaccgtctga tgaacgccgt ggctgcctac gcgaagtacc agtcggcctg catcgtggtg
    23701 gacttcggca cggccatcaa cttcgacatc gtgtccggca agggcgagta cgtcggcggt
    23761 gtgctcgctc ccggtgtcga gatctcgatg gacgccctcg cggcacgcgc ggcgaagctg
    23821 ccgaaggtgg atctcgagcc gccgcccgag gtgatcgggc gatcgacggt caccgcgctc
    23881 cagtcgggtt cgatctacgg cttctccggc atggtcgacg gcgtgctgcg gcgcgtgcgc
    23941 gaggcgctgg gcgagccggc gacgacggtc gccaccggcg gccacgcggc tgccataggc
    24001 ccacactgcg aggagatcga cgagctcgac gacctcctca cgctcacggg cctgcggcta
    24061 gtgtgggaaa agaaccgcca cggctagcct ttccggggcg atgaaccgat cgctcgacac
    24121 cccctggacg cttggcggca tcgagctgcc gaaccgcctg gtgctggcgc cgctcgccgg
    24181 catcgggaac tggttcgtgc ggctgcaggc gaagcgctac ggcgcgggcc tcgtcgtgtc
    24241 cgagatggtc tcgagcttcg ggctcaagta cggcgacgag cgcacgcacc gcgagttcct
    24301 gcgcctgcac ccggacgagc acccgatggc ggtgcagctg ttcggccacg acgccgccgt
    24361 gatgcgcgag gccgccgcga tcgtggccga ggcgggcgcg gacatcatcg acctcaactt
    24421 cggctgcccg gtacgcaagg tctgcaagac cggtgccggc tcggcgctgc tcgacgaccc
    24481 ggaccagggc gtggcaatcg cgcgcgccgc cgtcgagggc ggcggcggcc ttcccgtgag
    24541 cgtgaagatc cgcagcggga accgtcccgg cgagcgcacg ggcgtggagc tggctcagcg
    24601 gctggttcac gacgccggcg tgtccgcgat cggcttccac ccgcgccacg cctcgcagca
    24661 gcacaagggg cggcccgatt acgcgctcgc gagcgagctg gtcgagcggc tgccggtgcc
    24721 ggtgatgctc tccggcggct tgagcgacga agacgccacg ctggcggcgt tcgaggcatc
    24781 gggcgccgac gcggtgatgc tggctcgcgg ctcgctcggg aacccatggc gcttcgagcg
    24841 cctgctcggc cgccgccaga cgcctccggc cacggaggag gtcgtggagg agctgctgtg
    24901 ggtgatcgac caggccgagg accacctcgg ggttgagcgg gcaggccgct atttgcgcaa
    24961 gttctatccc tggtacgcgg acacgctcgg cctcaacaag cgcgagcgcc aggacctcgt
    25021 cacggcggcc acgacggacg acgcgagagc cgccctgcgg tcgctttcaa cccccatcgc
    25081 ggcctgatcc gtacccctat ttgcggtccg ccggagcccg tcgattacca caaatgcggg
    25141 tgctacactc tcgcgtccct ccggcccccc gagcggaggg ttttctaatg gcaaaggacg
    25201 tcattctcac cccagctggc ctcgaggatc tcaaggccaa gatcgactac ctctccaacg
    25261 atcggcgccg cgaggtggcc gagcggatca aggaggcccg cgagttcggc gacatctcgg
    25321 agaactccga gtacgacgac gcgaagaacg agcaggcgat gctcgagaag cagatcgcgg
    25381 acctcgagga gaagcttcgc aacgcgacgg tcatcgacga gaagacggtc tcaaccgagg
    25441 ccgtcggcgt cggcaacctc gtgcacgtga aggatcagaa gaccgacaag tcggtcaagt
    25501 accagatcgt gggctccgcc gaggcgaatc catccgagaa caagctctcg aatgagtcgc
    25561 cggtgggcaa ggcgctgctc gggcacaagc gcggcgacgt agtgtcggtg ccggtgcccc
    25621 gcggccccgc ccggaagctc aagatcacca aaatcgagac cgcttagggc ggctgcaaac
    25681 cttcgcctgg cggcgtcatc gcggccctcg tgtgcgtgcg cacacgtcgg accgctcttc
    25741 cttgccatgc tcggttttcg ctcgccctag ggcgggtgca tatagcctcc ccgcgcatcc
    25801 atgaccgacg ctggccataa cccgctcgac gaccgccgcg ccaagctcga gcgcctgcgc
    25861 gacgacgggg tcaacccctt tccgcacgag tacccgggcg tcgtcccgac gtccatcgtg
    25921 cgcgccgagc agggtggcct cgaaccgggc gaggaaaccg acgcgaagta ccgcgtggcc
    25981 ggccggctcg ccgcccgtcg cgggcacggc ggggccgcct tcctcgacct cgtggaccgc
    26041 tccggccgga tccagctgca cgcccagcgc gaccggctcg gcgaggaatc gttcgagcgt
    26101 ctcgtctcgc tcgacctggg cgacatcatc ggcgtcgacg gcaccgcctt catgagccgg
    26161 cgaggcgagc tcacgctccg gattgaggac tgggccctgc tcgccaagtc gcttcgcccg
    26221 ccgcccgaga agttccacgg cctcgaagac gtcgagaccc gccaccgccg ccgcgagctc
    26281 gacctgatgg cgaacgagga gacgcgtgag ctgttcatcc tgcgctcgcg tgtaatcgcc
    26341 gcgatacgtc gctggctcga cgagcgcggc ttcctggagg tggagacgcc ggtgctgcag
    26401 ccgctctacg gcggcgccct ggcgcgcccg ttcgtgactc accacaacca gctcaaccgc
    26461 gatttctacc tgcggattgc caccgagctc tacctcaagc gcctgatcgt gggcggcctc
    26521 gagcgggtct acgagatcgg caaggacttc cgcaacgagg gcatctcgca caagcacaac
    26581 cccgagttca cgatgctcga gtggtacgag gcctacgtcg actacggcga catcgccgct
    26641 gagctggagg acctcgtctc atatgtggca aacgaggtcg gctacgaggg cgagatcgac
    26701 ttcacgccgc cttggcgccg catcaccctg cgcgacgcga tcctcgagaa gaccgggatc
    26761 gacgtgctgg cgaaccggaa ccgcgacggg ctcttggcgg cggccgccga gaagggaatc
    26821 agcctgaacc ccaacgagac ctggggccag ctcgtagacg aactgctctc gaagtacgtc
    26881 gagcccgagc tgacccagcc aaccttcatc ttggactacc cggtggagct gtcgcccttc
    26941 gcgaaggcgc atcgcaccga acccgggctg gtcgagcgtt tcgaggcatt tgcagccgga
    27001 atggagttcg cgaacgcctt cacggagctg aacgacccgg acgaccagcg cgctcgcttc
    27061 gaagagcagg tccgacacac ggccgagggc gacgaggagg ctcagcccta cgacgaggac
    27121 ttcgtgcgcg ccctcgagca gggaatgccg cccacgggcg gcataggtgt ggggatcgac
    27181 cgcctcgtga tggtcctgtc gggccgccag tcgatccgcg acgtggtgct ctggccggcg
    27241 atgcggccgg agtagagggg ggcctcgaca gggtctctac tctggttgtg gggcctctga
    27301 accggcagcc agacaggctg ttacggccgg ttaaggcccg aagggccgag tagccccatt
    27361 gctagactga gcctaggctt cagggagtcg agggttcctt tatctcacgg cgaatgtgcc
    27421 gattaggaga caaagggctc ggggtacttc cctctggctc acccctccaa ccagaaggat
    27481 ttttaggtcg tgtttgagcg cttcaccgaa cgggctcgtc aagtggtcgt cctcgcacaa
    27541 gaggaggccc ggatcctgaa gcacaactac atcggcaccg agcacatcct gctcggcctg
    27601 cttcgggagg aggaaggcct tgctgcgcgt gtgctcgagt cgctcgacat caccgtcgag
    27661 cgcgtgcgcg cccaggtcgt ccggatcgtc gggtccggcg aggaggtcac gtccgggcag
    27721 atcccgttca caccacgtgc caagaaggtg ctggagctcg cgctgcgcga ggccctgtcc
    27781 cttggccaca actacatcgg caccgagcac atcctgctcg gcctcgttcg tgagaacgag
    27841 ggtgttgccg cccgcatcct gctcgacttc gacgcggact cggagaagat ccgcaacgag
    27901 gtaatccgca tgctctcggg cccgggtggc cgccgtcagg gccagcaggg cgggcagcag
    27961 ggcgaggcca agaagtcttc gaagctgctc gaccagttcg gccgcaacct caccaagctc
    28021 gctgcggagg gcaagctgga tccggtcgtc ggtcgcgaga ccgagatcga gcggatcatg
    28081 cagatcctct cgcgccgtac aaagaacaac ccggtgctga tcggcgagcc gggcgtcggc
    28141 aagaccgccg tggtggaggg cctggcccag cgcatcacga acggtgacgt gcccgagctg
    28201 ctgcgcaaca agcagatcta cacgctcgac ctcgcggcgc tggtcgcggg ctcgaagtac
    28261 cgcggtgagt tcgaggagcg cctcaagaag gtgatgaagg agatcaccca gcgcggcgac
    28321 atcatcctgt tcatcgacga gctccacaac ctcgtcgggg cgggtgccgc cgagggcgcg
    28381 atcgacgccg ccagcatcct caagccggct ctggctcgcg gtgagctgca gaccatcggc
    28441 gccacgacgc tcgacgagta ccgcaagtac ctcgagcgtg actcggcgct cgagcgtcgc
    28501 ttccagcaga tccgcgtcga ccagccctcg accgaggaga ccgtgcagat cctcaagggc
    28561 ctgcgcgacc gctacgagca gcaccaccgc gtcgccatca cggacgaggc gctggaggct
    28621 gccgcggagc tggccgaccg ctacatctcc gaccgcttcc tgcctgacaa ggcgatcgac
    28681 ctcatcgacg aggccgcctc acgcgcgcgc atcaagtcga tgacggctcc gcccgtgtac
    28741 cgcgagctcg aggacgacat cgagaacacg cggcgcgaga aggaggccgc gatcgaggcc
    28801 caggagttcg agaaggcggc gaacctacgc gaccaggagc ggcagctcac caacaagaag
    28861 cgtgagctgg aggagcagtg gcgccagggc gagtcgggca accgccccga cattggcgag
    28921 gaggagatcg cggacatcgt gtccatgtgg accggtatcc cggtgttcaa gctcaccgag
    28981 gccgagacgg cgaagctgct gcgcatggag gacgagctgc acaagcgcgt cgtcggccag
    29041 caggctgcga tcgaggccgt gtcgaaggcc atccgccgct cccgcgccgg catcaaggat
    29101 ccgaagcggc ccaccggctc gttcatcttc ctcggcccgt cgggcgttgg taagaccgag
    29161 ctggcgcgga cgctcgccga gttcctgttc ggcgacgagg acgcgatggt gcgcatcgac
    29221 atgtcggagt acatggagaa gcactccgtg tcgcgactgg tcggctcacc tccgggctac
    29281 atcggctacg acgagggcgg tcagctcacc gaggcagtcc ggcgcaagcc gtactcggtg
    29341 ctgctcctcg acgagatcga gaaggcacac ccggacgtct tcaacatcct gctccagatc
    29401 ctggaggacg gccgtctgac ggacgctcag ggccgcacgg tcgacttccg tcacgcgatc
    29461 gtgatcatga cgtcgaacat cggcgcctcc gagattgctc ggaacacgcc gctcggcttc
    29521 gccgtctccg acgacgagac cgggatcacg tacgacgaca tgaagaaccg gatcatgggc
    29581 gagctgaaga aggtgttcag gcccgagttc ctcaaccgaa tcgacgaggt catcgtcttc
    29641 cacaagctgt cgaaggacga ggtcaagtcg atcgtcgagc tgctgctgcg tcacatccgc
    29701 cagtcgatgg cggagcgcga gctgtcgctc aacctgtccg aggacgccaa ggatctcctg
    29761 gtggacaagg gctgggatcc cgccatgggc gcccgtccgc tgcgccgcgc gatccagcgc
    29821 tacatcgagg acccggtcgc cgacgaggtg ctgcggtcga cgatggagcc aggctccacg
    29881 gtccaggtcg acaaggcagg cgacgacgag gacccgccgg tgaagatcag catcaccgcg
    29941 cccacgaaga agcgcgaggc ggtcggggtc ggcgccaagg gcgacggcaa cgagggcggc
    30001 tcgctgcccg atgagccgga cgtcctgccc gaggtgcccg acgcgccgcc ggcggaagag
    30061 ccgccgaccg gcgagggtga cacctccgac gagggttagc gcccaagcat caacagcatt
    30121 cgagacggcc cgcttcggcg ggccgtttct ctttgcgccg ctagagtcgg gcgcccgtgg
    30181 cgccgaagac gacgaaggct tccgagcggc tcgcctgggc ggtcgacgcg ctggacgttc
    30241 agccccggga tcgcctacta gaggtcggat gcggccacgg ggtcgccgtc tccctggcgt
    30301 gcgagcggct tgacggcggc aggatcacgg cggtcgaccg ctcgccgaag atgatcgaga
    30361 tggcgtggcg gccctgcacc agcccggaag agcgctggag gtagtgaagg agcggctcgc
    30421 acccggcggc cgcctgtacc tcttcaacca ggagcccggg tggaagacgc cggagccggc
    30481 cgagcagttc ggtgcgcggc tgggcgacgt gctgtcaagc gctggattcg ggagcgaacg
    30541 cgtcgtcgtg aaggacttcg gcggtgccgc cgtcgccggc gtcgtagcct gctcggggcg
    30601 gtagggccag gctacgcgcg gctcgtcccg gcgaagcggt agagcaggtg gtgctcgccg
    30661 tcctggagcc aggagcgctc gagcgtgtag ttggcggtgc gcggcccgcg cttgagcgtc
    30721 ctgcggagcc aaccgcgggg cgcccacacc ttgcgcgtcg gcatccgtgc ttcgagcagc
    30781 tccatgcgtc ctgccacggg gccgtccgcg aaatgagcga tgaggttcac aggccctgcc
    30841 acggattcca aggtacgact tgtgccagcg cgccagccac caacatcgac gcatgtacta
    30901 cggcccgcgg caagggctcg caggctcggg cccggcggga ctagcggcgc atgtgcgctg
    30961 gcagcctcca tgcgaccccg gtatacccgg tttgagggag tcgtaagtct gacttgcgcc
    31021 cgcgcgggca tattgcgggt gttccccctg cactaggagc aatctgacga tggaacccca
    31081 agtcgagatc aagacccgcc tgaacggccc gtacaaggtc acgggccctg ttcgcatcat
    31141 cgacgccgac ggcaacgagt tcgacctcgc agagcacggc gaggtgatcg cgctctgccg
    31201 ctgcggtggc tcttccacca agccgttctg cgacggcact cacaagcgca atggcttcgc
    31261 tgccgcggag aaggcggtcg tcacgggggc ggcggaagga tgagggacct gcgggacgat
    31321 cttggccgac cgcccgcagg tccctcgagg gagacagagg cgatgacacc gcgcggccac
    31381 cccgcccggc acgtatcgct gtgttccgaa caagccgcac actcgagcgg ctgagctgat
    31441 attcgccgcg agagggccgc ggcacttccg cccgctgtcc agaaatcgtc cgccggggtt
    31501 cgcccgcgag tcgaatcgcg cggtagcaag cctgccaagg ctcgagccgc gcggttacga
    31561 gttgcgctct tcgtcttccc cgcgccgcag gaactcgggt gtcgcgatga ggaacacgcc
    31621 ccaggcgacg aagccgagca gggcgatgat cccgagcgtg accgtcaccg gaaactcgaa
    31681 cggcaccagc agtgcaacgc cgagcgcgcc aagggccagc catccgcgcc gcgcggtcat
    31741 gagcgccagc gcatgtcgag gggttggtag ggctggtcga gccggccgcg cagcaccagt
    31801 cggtacagca ggaacagcga gggcagcacc agcgcgaggc cggcccccgc ggcgatcacc
    31861 agcgcgacga gcgttgcgtt gctcgcggcg gcctggtcga gcgtgagctc gcccggtagg
    31921 aggtacggac tctgtgcggc ggcccagccg gcgatcacgc acgctacggc agcagccgct
    31981 gtgaatcggg cgacggtcag gcgttcggtc cagaccagga cgagcgtggc gatgccgaga
    32041 attgccgacg cgattacgag cgcgagcccc gcgccggacg tcaggccgtc gtagagctcc
    32101 cgcgcatcgc tgcgcacgac cgcgagcgcg ccgagcgcca cggcccctgc tacgacgccg
    32161 gagatgagcg ctcggcgtct cgttgcacgt tcgagctcgg gctcaccggc acgttccgcg
    32221 tcgcccgcga ggaagatggc cgcgaggtac gcgccagtga tgactgcgag cactccaacc
    32281 gtgatcgagg tcgggttgag ccatgagctc catggatcac cgccggcgtt gccgaccgga
    32341 acgcggcccg acgcgatgcc gccgatcgca gcgccgaagc aaaacggcac gagcaccgag
    32401 gacacggcga acccggcgcc gaacagtcgc gcctgcccga ggctcgtcgc gtgcgggcgc
    32461 aagacgaacg ctgtgccccg caggatgatg ccggcggtgg cgccgaacaa cggaatgtag
    32521 agcgtcgacg tcaccgagcc gaagaagcgc gggaacgccg tccacagcac caccagcaga
    32581 aagggcaacc agacgtggtt tgcctcccag accggcgtta tggatctctc cagcagtcct
    32641 cgcagcgggc cgccgcgctg agcgccgccg gcggtcagat cccagaaccc gccgccgaag
    32701 tctgcgccgc cgagaaccac gtacgcggta agcgtggcca gtgccaggat caggcaggtt
    32761 tcggccatgg cgccccttcc gtgtcagaga gctcggcctc cggtggccgg gccgcgagcc
    32821 ggcgcgtcat ccacacggcc accgccgcaa gaccgaggta gacgatgagc atgccgaaga
    32881 agaccacggg caatcctcca gcattggtca cggcttgctt ggtgcgcatg acgtcgtaga
    32941 cgatccatgg ctggcggccg acctcagttg tgacccaccc gcagatcagc gcgacgaccg
    33001 cagccggccc agccaccacg acggcccggt agaaccacgg cgtgcgcggg agccggccgt
    33061 gtcgccacca ggtgagcacg aacacgacgc cgagcaccgc gagcagcgtc ccgattccga
    33121 ccatcaactg aaacgcgtag cggacggtgt tgactggtgg gcggtcctgc ggcggcacct
    33181 cttcgagccc ttggatcctc gcgttcgggt cgtgcttcgc cagcactgaa aggaggttgg
    33241 gaagcgggat gccgaacttg accttgttgt cgtggtagat gccaccgaca tggatcggtg
    33301 ctccgtgctc cgagtgatag agcccttcga aggccgcgag cttcgtcggc tggtagttgg
    33361 cgaccgtccg cccgttccag tcgcccacaa gcacctgcac cggcgcggcg agcgcggccg
    33421 cggtcaacgg caccgccagc gcggcgcgca catagcgtga ccgatcgccg cgcagccagg
    33481 acacggcgta aatgcccgca acgacgaacc cggcgaccat gaacgcggcg aagtacatgt
    33541 ggacgaactc atgccagaag ttcgggttga acagcgcctt ccagggccgg acgttggtaa
    33601 ctcggccgcc gggggtcacg tcgaagccca ccggatgatt catccagccg ttgacggaga
    33661 tgaccatcag cgaccccgtg atcccagtca cgacgatcgg cactcccgac agcagatgaa
    33721 ctttcggggg aagacgctcc cagccataga cgtagatggc gatgaagatc gcctcgatga
    33781 agaaggaagt gccctcgatc ccgaaggcca ccccgaagac gtcgccgaac gtgcccgtga
    33841 accctggcca caagagcccg agctcgaagg acaggatcgt gccggtgacg accccgatcg
    33901 cgaacaggac ggtcatcgcc ttcgaccagc gcttggcgag cgcgcggtag gcggggtcgc
    33961 cggtgcggag cgagagccct tcgacgaaca gcacgatcgc cgggaagccc accccgaagc
    34021 agacgagcgg gatgtggaag gcgaacgaca tcgcctgcag ctcacgcgcc cacaggagat
    34081 gctgctggtc gaccgggcca agcgccggag cggcgctctg tgctgccgcc agcacgctgg
    34141 tcagggccat cctcggtgag ggtatccaac gcattgcact cgcctgcctg ctgcgggggg
    34201 tagggcgaaa acgtccgctc ccgtccttac cttcgacggg tggcaaagac cgtcttcgtt
    34261 tgttcgtcgt gcgccgctga gagcgccaag tggcatggcc gctgcccgag ctgcggggag
    34321 tggaacacga tggtcgagga gcgcgccgcc gcgcccccgc gcggcgcgcc gagcggcggg
    34381 ggggcgaagc gcgccgcgcg ggcgctcaag ccggtgctgc tctccgaggt cgaggcgccg
    34441 cacgtcgagc gcttctcgac cggcatcgcg gagctcgacc gcgttcttgg cggcggcatc
    34501 gtgcccggct cgctggtcct gatcggcggc tcgccgggca tcgggaagtc gacgctcacc
    34561 tcgatggcgc tcggcaacct gcagcaggcg ggcaagcgca cgctgtacgt gtccggcgag
    34621 gagtcggcgg cgcagatccg gctgcgcgcc gagcggctgc cgggcgcggc gctcgaggtg
    34681 ccgacgctgg ccgagaccga cctcgacacg gtcttggcca cgctggacgc cgagcagccc
    34741 gaggtctgcg tgatcgactc ggtccagacg ctccacgccg ccgacctgac cggcgcggcc
    34801 ggctcggtgg gccaggtccg cgaggtcgcc gaccggatca cgcggctggc caaggcgcgc
    34861 gacatcgcgg tgctgctcgt cggccacgtc accaaggagg gcgcgctggc cggcccgcgc
    34921 gtgctcgagc acctcgtcga ctgcgtgctg cagttcgagg gcgagcgcga gcgcacctac
    34981 cgcacgctgc gcgcgctcaa gaaccgcttc gggtcgacca acgacgtcgg cgtcttcgag
    35041 atgcgccaag gcggtctcgt cgaggtcgag gacgcctcgg cgcggttcgt gggcgaggcc
    35101 acgcgcgcgc cgggcagcgc cgtgctcgcc gccatggagg gctcccggcc gctgctggtc
    35161 gaggtccagg cgctggtcgc gccgaccgag gtcgtgccgc cgcgccgtgt cgccaccggc
    35221 atcgatcgga accgcctggc catggtgatc gctgtcctcg cgcggcatgg ggggccgtcc
    35281 ctggcctcgg ctgacgtctt cgtgaatgtg gccgggggcg tccgcgtgga cgagcccggg
    35341 gcggacctgg ccgtggccct ggcgctcgcg tcggcccacc ggggagagac gctcgccgat
    35401 gcc
//
LOCUS       DASVXS010000228        18735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1407665, whole genome shotgun sequence.
ACCESSION   DASVXS010000228 DASVXS010000000
VERSION     DASVXS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407665"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..119
                     /locus_tag="VF752_12810"
     tRNA            35..119
                     /locus_tag="VF752_12810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69..71,aa:Leu,seq:tag)
     gene            330..893
                     /locus_tag="VF752_12815"
     CDS             330..893
                     /locus_tag="VF752_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8856470.1"
                     /translation="MLIYSMSVSVDGFIADREGAFEWGAPSEELFRFHTALVSDLGGY
                     LLGRRLYETMLVWETDPSMRDNELRATFADVWCAIPKVVFSHTLDSVQGNARLAEASL
                     AEEIAAALDATDKDVSIGGAGLAAQAIELGLVDELRIFRYPVVVGGGTPFLPPVTEDV
                     PLDLIDTRTFGSRVIYERYGRARDESD"
     gene            955..1158
                     /locus_tag="VF752_12820"
     CDS             955..1158
                     /locus_tag="VF752_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8856471.1"
                     /translation="MPTPIEIDRLQELLGGGAQLVDVLPTRVYGEQHLPGAVNIPLTQ
                     LDRETTAVLDKRSAVVVYCWDYI"
     gene            1210..1635
                     /locus_tag="VF752_12825"
     CDS             1210..1635
                     /locus_tag="VF752_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8856472.1"
                     /translation="MYDYAPGKVDWLARNLPSEGELASRPTAGSFVRRDVATCQLDQP
                     VGPVRERIDETPYGFALVLSPKGVLLGRLRRSALDGDPNAVAESVMEPGPTTVRPDTP
                     AGELAERLRKKDLKTAILSDPEGRLLGIVLRRELEADAV"
     gene            complement(1648..2307)
                     /locus_tag="VF752_12830"
     CDS             complement(1648..2307)
                     /locus_tag="VF752_12830"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX8856473.1"
                     /translation="MWREGLLQMGSFFQVMPLAFVMVAGPQILSPIFLATTAGWRKNS
                     AAYVLGAAISIAIVVVAAYFIVKGLKSTSSASKSTKSNVVDYVILGLLAFLIVHVYLT
                     RKTAKKPKWMDRLQRATPRFAFTLGFVLLGFFPTDIVSSVTVGSRLAGHNQPLWQALG
                     FYGLTLLLIAVPALMVLVMGKRAETLLPKIRDWMDTNSWVVNEIVLLFFVAIEIKSVV
                     S"
     gene            2395..3195
                     /locus_tag="VF752_12835"
     CDS             2395..3195
                     /locus_tag="VF752_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEX8856474.1"
                     /translation="MRFLPALATAATLALPVTASAQQSPTDANPCLDTATYADLLCPD
                     LIMHPPSDLRVDRRTRPGRVLLRATNWIENIGTGPVELRGIRDGARTMSAHQAIKRRV
                     LVPLRLPPSARLYFFPIPGQYRYWKFRDAARFELWSTYKNGQARRLVRHGPKQYYCLR
                     DLRRTHPSSISPRRFVYAGCNQNPNARRVTLGTSPGWADIYPASYHQQWIDVTGLRGR
                     FLYRQIVDPKNHIAESNETNNVGEVYVNLPYRGNVRNRSPFYSTSRSR"
     gene            complement(3197..3868)
                     /locus_tag="VF752_12840"
     CDS             complement(3197..3868)
                     /locus_tag="VF752_12840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8856475.1"
                     /translation="MLPEAILFDIDGTLINSGGAGARSWAFAFERLYGHPVDIGAFTD
                     AGMTDPEVGRLTFAKDQGHEPSPRELARLIHTYLSVLPDFVSESEGYRVLPGVQDLLP
                     RLMDEGVFLGITTGAVEAAAHVKLGRGRLNHFFSFGGYGSDSADRIELTERAFHRGRN
                     VAGHELDPKRTMVVGDTPLDVKAAQGAGLIAIGVASHHNTKEELAAAGADYVLGSLEE
                     PLPEL"
     gene            3904..4242
                     /locus_tag="VF752_12845"
     CDS             3904..4242
                     /locus_tag="VF752_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856476.1"
                     /translation="MSVEVRPGDVPACVRFYELVGFAHVEAPETLKGVATWLQWGRTQ
                     VHLLHTDSPVNPPAGHLAVVVEDYDATVERLRSAGFEVDPHREHWGVPRSFVSDPAGN
                     LVELMAAPPA"
     gene            4287..4793
                     /locus_tag="VF752_12850"
     CDS             4287..4793
                     /locus_tag="VF752_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856477.1"
                     /translation="MEASSSVEIRHPNRDTAAAKATKAAVILLLLTSAGLILIVTVGG
                     WDNLQGAQVVAIAWLLIYVLMAYYVGRWNRGVLPVASAMAILLAVLAAVAAPSWFDRD
                     KSGFDNPGLPPGILGLLCVLLIPVSLLLIAFAMRGFQQKWNVEVEVPRGEEPPYGDTR
                     SEPRPVAG"
     gene            complement(4794..4876)
                     /locus_tag="VF752_12855"
     tRNA            complement(4794..4876)
                     /locus_tag="VF752_12855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4841..4843),aa:Leu,seq:caa)
     gene            complement(4899..5336)
                     /locus_tag="VF752_12860"
     CDS             complement(4899..5336)
                     /locus_tag="VF752_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856478.1"
                     /translation="MDTSRVSLGEWIAAVAAAALFLFLFLTWSASLSAWELFDFVDVI
                     LAVIALVVLAIVLIHAGGREVALPIERDRLIAGLGGVAFVIVLSFLLEASDRGAGIWL
                     ALLATIAIVYGGLSATRGADAALGTRPRPRTRPVPPPADRDAL"
     gene            5454..6686
                     /locus_tag="VF752_12865"
     CDS             5454..6686
                     /locus_tag="VF752_12865"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8856479.1"
                     /translation="MREIDALVEFRGRAAGTDAERRAAEHLANRLREIGRDADVEPVH
                     VYPNYPVTHAIHALLAIAGSVLSVSHPAVGAVLVLVAMLSALGDLTGVFYAVRRLTGR
                     RASQNVFSRVDAGRDGTLVLVAHYDSAHSGAVFGGRVQERRAVLGKLLKRPIGLFEPF
                     FWSIVAVLACTILRVAGIDALLLTIVQFIFTVVLIVSVPLLLDIALSDPVPGANDNAS
                     GVATVLRLAERFGYVLDHFQVCVLFTGAEEGLLGGMREWMRRHRKQLAPERTVFLNVD
                     KVGTGTVRFTTKEGLVIASPYHPTLISLCHEIADEDDEGGRYGARGLVSRHVTDAYAA
                     RTAGFPAITISCANALDYVPEYHQHSDTIDHIDSEALDRAYGFCCELIERLDARVGAD
                     LAAARSDQETALSEEETV"
     gene            complement(6935..9574)
                     /locus_tag="VF752_12870"
     CDS             complement(6935..9574)
                     /locus_tag="VF752_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856480.1"
                     /translation="MPITASGKIYNVEPGPDEPARLGAIVNSLPLGGIPGFTLKIPVE
                     VAVRPTDYGLDTTIDEIPRTAPGGVSLNVKQMDITLDGTPAGGSGPFMTNPTRCGAAT
                     TTVDAASYAAPASFSSNSATYNVTGCSSLPTFAPTLSVTRASSRADTPTAYTIAVNVP
                     GGQSHVKRTEVVLPRGTTLSPPSAEGMSACTDGQFAKATADTVACPGNSELGTVTINT
                     PLLGVLNGKVWLGTPTADAPLRLFVGVQEHGLSIKVIGLVTPNPADGQVTTVFDDLPQ
                     TPFTTFALSFRGGDRAILANPVECGSYTATARVTPWSGQPDATPTSAPFPVDDDGAGG
                     ACPAKRPFTPTLQAIPGNTQAGHDPGSLDLVVDRPDRDQRLKGLKFSLAPGLLGKVAG
                     VPLCGNADAATGNCPANSRVGTVTAAVGSGGAPTPLSGPVYFGGPYNGGIASLIVVLP
                     GKVGPLDVGTTVLRSALKLRGTDGGIDVTSDDLPQFVGGIPTAIRHLSVKLDRPGMLL
                     NPTSCAPLQVVGHFTSAEGDTADPTAPFQATGCEKLPFNPGFSAVAGGRGETNQLRHP
                     FLRTVITQKEGEAAQKSVAVTLPPTIGPDLEAVGRACPQAVAATATCPPSAKVGTATA
                     SSPLLPLPLAGPVYLVQIPGQAFPGVLLDLKGLLDLKLNGRVTGTQKGLVTVFDSVPD
                     VPIGKFELAFDGGPKGSLVAVRDLCASTQKVSAAFTAHSGATRTVTSPLLVSGCATKA
                     SLTLSGVKKKKPRLDLRTRGFPNSAGFERLELRMPRSLKGVSRKAKRGVRVLSGSKRV
                     RGMKATLRGSRLVLTGLPKDTQSLRVIVIGGALKPAKSLLSAFQRHRKPRLTFKLLTR
                     EAGERSGTSTAAPAKALTIRPRS"
     gene            10448..10945
                     /locus_tag="VF752_12875"
     CDS             10448..10945
                     /locus_tag="VF752_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856481.1"
                     /translation="MLADSAALALTRAGALAGEKLERVFRRLGCHVRDYAVLELLAAT
                     DPGPIYDGPARLFGVRIGMAQSRLRKRIGLSPAALSRLLREMETAGFINLGFGRDGRE
                     RFVELKPLGKERLTPCREALRASEEEIFEGIDADRREQLRETCLQLPPLGGRDRREEW
                     WLRRG"
     gene            complement(10932..12455)
                     /locus_tag="VF752_12880"
     CDS             complement(10932..12455)
                     /locus_tag="VF752_12880"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8856482.1"
                     /translation="MSDLREALERALGGGPERHRSKAAEQGKLPVRERIGRLIDADSF
                     AEEALLAHHEEEGFGADGVVTGMARVGGRAVCLMANDPTVKAGSWGPKTVEKIIRIQE
                     QALLHEVPMIYLVDSAGARITEQVQMFPGRRGAGRIFHTQVKLSGVVPQVCVLFGPSA
                     AGGAYIPAFCDVVIMRDGNASMYLGSPRMAEMVIGEKVSLEEMGGARMHTGVSGVGHF
                     LAKSDEEAIETAKRYLGFMPSNWREQPPHAPPAPPPADAVPVADLVPADENKAFDMGQ
                     LIDGLVDQGSFFEVQKRWAKELLVGYARLDGRAIGIVANQPKKRGGVLFVDSSDKAAR
                     FIWTCNAFNIPLLFLADVPGFMIGTQVEKQGIIRAGAKMISAVSEATVPKISVIVRKA
                     YGAGLYAMAGPAFEPDACIALPWAKIAVMGPQAAINAVYYNQVQAIEDPDEREAFVAK
                     LREEYAEEIDILHLASELVVDAVVQPEDLRTDLVRRYELIASRRRVWPPKNNPISPV"
     gene            complement(12460..13344)
                     /locus_tag="VF752_12885"
     CDS             complement(12460..13344)
                     /locus_tag="VF752_12885"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8856483.1"
                     /translation="MEISKVGVLGAGLMGHGIAQVAAQSGYDVVLREVDQERLDKGLG
                     RIQKQLARAVEKGRMEQAEADAVLGRIHGTTDYNDLADSDLVVEAITEDLGLKLEMWR
                     ELDPIVKDGAVLATNTSSLSVIDQAAATRRPERFVGLHFFNPVQQMALLEVVKTVTTD
                     DESLAAGFAFGEKLGKLTVPAKDKTGFIVNRLLVPYMLDAIRAYEEGVAPIEEIDAAM
                     KAGAGHPMGPLLLSDFVGLDTLKSIADIMFDEYRERRFAAPPTLRKMVSAGWYGRKSG
                     RGFYDYSGEKPVAVDPGL"
     gene            13394..14173
                     /locus_tag="VF752_12890"
     CDS             13394..14173
                     /locus_tag="VF752_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8856484.1"
                     /translation="MGLVAVEDRDAVRHVVMERAEKRNALNDELVLALGEAFEEAAGD
                     DSVRVVVLRGDGPMFSSGMDLSGLRDLSENPDGLREFRAPLIRIYNMLEEMPKATIAQ
                     IHGACIGGAMELALACDMRTMAEDAVCGLLETRVGLIPDLGGCSRLPSVVGAGIAKEL
                     IMTGKVIDGREAHRIGFANRIAPAEELDAATDQLVNELLAAAPKAVALAKRVIDQAAK
                     PDLERSLEREEDAQAELAASADFAEGTKAFFEKRQPEFAGR"
     gene            complement(14170..15411)
                     /locus_tag="VF752_12895"
     CDS             complement(14170..15411)
                     /locus_tag="VF752_12895"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8856485.1"
                     /translation="MSTTRRFVIVGAGLAGAKAAETLREEGFDGELLLLDAEHERPYE
                     RPPLSKEYLRGEAGRDKVYAHEPGFYEEHAIDLRPRTSAAALDTGASEVVLDSGERIS
                     YDAALISTGSSPRRLNIPGMTLDGVHYLRDVEDSDELRELFSRGGRLVAVGAGWIGCE
                     VSASARQMGMEVALLDPLRTPLERVLGEEVGGIYADVHREHGVEVLMETGIERIEGSG
                     RAERVITSDGRALDCDLVLIGVGAVPRIGIAEAAGIDVGDGGILVDASLRTSVPNVFA
                     AGDVANELHPITGARVRVEHWANALNQGPAAARAMLGQPVSYDRVPYFFSDQYDVGME
                     YSGWPTPWDELVIRGDQETRELIAFWLKDGSVIAGLNMNVWDVTDPIQELVRSWGRMD
                     PARLADVETPLEELVAELSKA"
     gene            complement(15422..16030)
                     /gene="folE"
                     /locus_tag="VF752_12900"
     CDS             complement(15422..16030)
                     /gene="folE"
                     /locus_tag="VF752_12900"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX8856486.1"
                     /translation="MTERPNLHVVTGDSTSIDVDAAERAARDLLEALGADLDHDGLRD
                     TPRRVARTYAELLTPQPFNLTTFENEEGYDELVLARAIPFHSLCMHHLLPFHGVAHVA
                     YLPGDRILGLSKLARVVDMFARGLQVQERLTKQIAGWLEDALHPKGVGVVLEAEHMCM
                     TLRGVQKPGTRTVTSALYGLVRDDPRTRQEFLALAGCSPHPV"
     gene            complement(16027..16587)
                     /locus_tag="VF752_12905"
     CDS             complement(16027..16587)
                     /locus_tag="VF752_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8856487.1"
                     /translation="MSPTTFESVADPARRRILDHLGERPGSTLQELADAAGVHLNTAK
                     PHVAALERAGLIEGERATPAGRGRPPIRYRLAEGYELPTADFRGLAEVLAMAVDRNEP
                     VEAAAREWGCRCGDLPRALERLGFDARITGDTLRLSACPCPLVLPDHPELLCGVALGV
                     VEGSGARVVESSHDPERRRCEAKLSR"
     gene            complement(16659..17774)
                     /locus_tag="VF752_12910"
     CDS             complement(16659..17774)
                     /locus_tag="VF752_12910"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8856488.1"
                     /translation="MTRTDIEALCAIARGSATAGERQSAEWIAGRLERIGAHDVRLAS
                     FRYQPSWADRQLPHFAAGVLAAALKSRTLALGTLASFALEFSGRAQWLARVMPAGEGT
                     NVVATLPAAGEPRRHLVLVAHHDAARSGLMWHPALVRHSFRHALDRGRVDSFALPPSL
                     ALAAVATGTRAGRALGGAVLALGSALAAGAARGAVVPGANDNATGVAAVLALAGRFAA
                     EPLPGTDVTLLFPGCEEAGMGGMAHWLAQNRGRLDPGATLVLGLDTLAAGEPCVLTGE
                     ALPHTVHYRAEDLALADLGAERAGTEQPRRLRLGAWTDPILAVYAGLPAILMLSIGPE
                     GFGPYHRLDDTPERVDWRSVDSCLRLAEGIARAFAGL"
     gene            complement(17771..>18735)
                     /locus_tag="VF752_12915"
     CDS             complement(17771..>18735)
                     /locus_tag="VF752_12915"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX8856489.1"
                     /translation="VLARPRRGTLPIAFPAPVAEQVKVREVGPRDGFQNEPEVIATAD
                     KVRLIDMLARTGVPRLEVTSFVRPDVIPQLSDAREVLGAVDIPREVAVTVLIPNERGL
                     EKALELRDRFDEVNLFLSASETHNQKNVNRSIEESLSGLERVIAHARADGLRCEGVIS
                     VSFGCPYEGEVPPERVFGIAERLVAAGAEEIAFGDTTGMANPRQVRAFFAAAANRLPG
                     VQLTAHFHNTRGQGLANVVAALDEGVRSFESSFGELGGCPVPKGATGNIATEDLVSML
                     HEMGYYTGVDLEKLLAATRAVQEVLGRTLTGHLLKAGPVDWHRG"
BASE COUNT         2900 a         6569 c         6348 g         2918 t
ORIGIN      
        1 agacacgggt cgggcctaga atcgggcggc gcctgcggat gtggcggaat tggtagacgc
       61 gccggcctta ggagccggtg ggccgaaagc tcgtgggggt tcgagtccct ccatccgcat
      121 caaccagtcg aggacgaacg aggcgttcgg gcggcgtcgg caatggccgt acgatcgctg
      181 aaccgcccac gacgatgagt tttcgtctcg cgatcggtca ctagctgtgg cggagcaacc
      241 tgcagaagga aggcgtgccc gatgtcaagc tcaatggtga acttcaccgc gatcatcgtc
      301 cgtcgctccg gtgcgatggg cttgaagcga tgctgatcta ctcgatgagc gtctcggtgg
      361 acggcttcat cgccgaccgc gagggcgcgt ttgagtgggg ggcccctagc gaggagctgt
      421 ttcgtttcca caccgcgctg gtaagcgatc tcggcgggta tctactcggc cgcaggctct
      481 acgagacgat gcttgtgtgg gagacggatc cgtcgatgcg cgacaacgag ctcagggcta
      541 cgttcgccga cgtctggtgc gcgatcccga aggtcgtctt cagccacacg ctcgacagcg
      601 tccagggcaa cgcccggctc gccgaggcgt cattggccga ggagatcgcc gcggcgctcg
      661 acgcgaccga caaggacgtc tcgatcggcg gcgccggcct ggccgcgcag gcgatcgagc
      721 tcggcctcgt cgacgagctg cgcatcttcc gctacccggt cgtcgtcggt ggcggcacgc
      781 ccttcctgcc gccggtcacc gaagacgtcc cgctcgacct gatcgatacc aggacgttcg
      841 gctcgcgcgt gatctacgag cgctacgggc gcgcccgcga cgagtcggac tgacaccggc
      901 gttcgagtca tcagcgcctc gccggaccgg gtaataatcg aacgaggagt cgctgtgccc
      961 acgcccatcg agatcgaccg gctacaagaa ctactcgggg gcggagctca gctggtggac
     1021 gtcttgccga cccgcgtgta cggcgagcag catctgcccg gcgccgtcaa catcccgctc
     1081 acgcagctcg accgcgagac caccgcggtg ctggacaaac gcagcgcggt cgtcgtgtac
     1141 tgctgggact acatctgaga cctgagtccg cgagccgcgt gccggctcga aacgctcgga
     1201 ttcgaccagg tgtacgacta cgcgcccggg aaggtcgact ggctggcacg caatcttccg
     1261 tccgagggtg agctggcctc gcgcccgacg gcgggcagct tcgtccgccg cgacgttgct
     1321 acgtgtcagc ttgatcagcc ggtgggtccg gtccgcgaac ggatcgatga gacgccgtac
     1381 gggtttgcgc tggtgctcag tccgaagggc gtgcttctgg ggcgccttcg ccggagcgcg
     1441 ctcgacggcg atcccaacgc cgtcgccgag tccgtgatgg aacccggtcc gacgaccgtg
     1501 cgcccggaca cgccggcggg cgagctcgcc gaacggctcc gcaagaagga tctgaagacg
     1561 gcgatcctca gcgacccgga aggacgtctt ctcggcatcg ttctccgacg cgagctcgag
     1621 gcagacgccg tctgatcgcg tgcgctgtca gctcacgacg ctcttgatct cgatcgcgac
     1681 gaagaacagc agcacgatct cgttgaccac ccacgagttc gtgtccatcc agtcgcggat
     1741 cttgggaagc agggtctcgg cgcgctttcc catcaccagc accatcagcg caggaaccgc
     1801 gatcagaagc agcgtgagcc cgtagaaccc gagcgcctgc cacagcggct ggttgtggcc
     1861 cgcgagacgg ctgccgactg tcacggacga aacgatgtcg gtggggaaga acccaagcag
     1921 cacgaagccg agcgtgaatg cgaacctcgg agtcgcacgc tgcaagcgat ccatccactt
     1981 cggcttcttc gctgtcttgc gtgtcagata cacgtgcacg atcaggaacg cgagtagacc
     2041 aaggatcacg tagtcgacca cgttgctctt ggtgctcttc gacgcgctgc tcgtgctctt
     2101 cagccctttg acgatgaaat acgcggcgac aacgacgatc gcgatcgaga tcgccgcgcc
     2161 gagcacgtag gcggccgagt tctttcgcca cccggcggtc gtcgcgagga agatcgggct
     2221 gaggatctga gggcccgcga ccatcacgaa cgcgagcggc atgacttgga agaagctgcc
     2281 catctggagc agaccttcgc gccacacgga cgtcgcgtca tcaccccaag ggggtgaacg
     2341 tcgggcccgc cggagaggcg catcgatcgt ccgacttgta cgttggaaca agggatgcgc
     2401 ttcctccccg ctctggccac cgcggcgacg ctcgccctac ccgtgaccgc ttccgctcag
     2461 caatcgccga cggacgcgaa cccatgcctg gacaccgcca cctacgcgga cctgctctgc
     2521 ccagacctga tcatgcaccc gccgagcgac ctccgcgtgg accgccgcac gcgccccggg
     2581 cgcgtactcc tgcgcgcaac gaactggatc gagaacatcg gcaccggccc ggtggagctg
     2641 cgcggaatac gcgacggcgc gcgcacgatg agcgctcacc aggcgatcaa gcgccgggtc
     2701 ctcgttccgc ttcggctgcc cccgtcagcg cgcttgtact tcttcccgat ccccggccag
     2761 taccgctatt ggaagttccg cgacgccgcc cgcttcgagc tctggtccac ctacaagaac
     2821 gggcaggcgc ggcggctcgt acggcacggc ccgaagcagt actactgcct gcgcgacctg
     2881 cggcgcaccc acccgagctc gatctcgccg cgccgcttcg tctacgcagg gtgcaaccag
     2941 aatccgaacg cacgtcgcgt cacgctcgga acctcacccg ggtgggccga catctacccc
     3001 gcgtcatacc accagcagtg gatcgacgtg acgggcctgc gcgggcgctt cctctaccgg
     3061 cagatcgtcg atcctaagaa ccacatcgcg gagtcgaacg agacgaacaa cgttggcgag
     3121 gtgtacgtga acctgccgta ccgcggcaac gtgaggaacc gctcgccttt ctactcgacc
     3181 tcgcggtccc ggtagctcag agctcgggca gcggttcttc gagcgagccg agcacgtagt
     3241 ccgcgcccgc cgcggcgagc tcctccttgg tgttgtggtg gctcgcgaca ccgatcgcga
     3301 tcagacccgc gccctgcgcg gctttcacgt cgagcggcgt gtcgccgacg accatcgtgc
     3361 gcttgggatc gagctcgtgg ccggcaacgt tgcgtccgcg gtggaacgcg cgctcggtga
     3421 gctcgatgcg gtcggccgag tccgagccat atccgccgaa ggagaagaag tgattcaggc
     3481 gcccgcggcc gagcttcacg tgcgcggctg cctcgacggc gccggtcgtg atgccgagga
     3541 acacgccctc gtccatcagc cgcggcagca ggtcctggac ccccggcagc acgcggtacc
     3601 cctcggactc ggatacgaag tcgggcaaca ctgaaaggta ggtgtggatg aggcgagcga
     3661 gctcgcgcgg actgggctcg tgaccctggt ccttcgcgaa ggtgaggcgc cccacctccg
     3721 ggtcggtcat gccggcgtcg gtgaaggcgc cgatgtcgac ggggtgtccg tacaggcgct
     3781 cgaacgcgaa cgcccagctg cgtgccccgg cgccgcccga gttgatgagc gtgccgtcga
     3841 tgtcgaacag aatcgcctca ggcagcatgc ctgcgtaccc tacgagaccg gatacttcag
     3901 cacgtgagcg tggaggttcg gcccggcgac gtccccgcgt gcgtgcgctt ctacgagctc
     3961 gtcggcttcg cgcacgtcga ggcgcccgag acactcaagg gcgtggcgac gtggctgcag
     4021 tggggacgca ctcaggtgca tcttctgcac accgacagcc cggtcaaccc gcccgcggga
     4081 catctcgccg tggttgtcga ggactacgac gcgacggtcg agcgcctgcg ctcggccggc
     4141 ttcgaggtgg acccgcaccg ggagcattgg ggtgtgccgc gctcgttcgt cagcgacccg
     4201 gcgggcaacc tcgtggagct gatggcagcg ccgcccgcct gaaccagcac atcggcgccc
     4261 ccgttcggct tacacttctc cccctgatgg aggcgtcgtc ttctgtcgag attcggcatc
     4321 ccaaccggga caccgccgcc gccaaggcca cgaaggccgc ggtcatcctg ctgctgctca
     4381 cgagcgcggg gttgatcctg atcgttacgg tgggcggctg ggacaacctc cagggcgctc
     4441 aggtcgtggc gatcgcgtgg ctgctcatct acgtgctgat ggcgtactac gtcgggcgtt
     4501 ggaaccgcgg cgtgctgccg gtggcctccg cgatggcgat cctgctcgcc gtcctcgcgg
     4561 cggtcgccgc gccgagctgg ttcgaccgcg acaagagcgg cttcgacaac cccggcctgc
     4621 cgcccggaat cctcggcctg ctctgcgtgc tgctcatccc cgtctccctg ctgctgatcg
     4681 ctttcgcgat gcgcggcttc cagcagaagt ggaacgtgga agtagaagtg ccgcgcggcg
     4741 aagagccgcc gtacggagac acgcgcagcg aaccccgccc agtggcgggc tgatacccgg
     4801 ggggggattc gaacccccac gccctctcgg gcagagcatt ttgagtgctc cgcgtctgcc
     4861 gttccgccac ccgggcgggc tcgcttagcg tagccgcgct agagcgcgtc gcggtccgcg
     4921 ggcggcggca caggccgggt ccgtgggcgc ggccgcgtgc cgagcgccgc gtcggcgccg
     4981 cgggtggcgc tcaagccgcc gtacacgatc gcgatcgtgg cgagcagcgc cagccagatg
     5041 cccgcgccac ggtcgctcgc ctcgagcagg aagctcagca cgatcacgaa cgcgacgccg
     5101 ccgaggccgg cgatgagccg gtcgcgctcg atcgggagcg ccacctcgcg gcctcccgcg
     5161 tgtatgagca cgatcgcgag cacgaccagg gcaatcacgg ccaggatcac gtcgacgaag
     5221 tcgaacagct cccacgcgct gaggctcgcg ctccacgtga ggaacagaaa cagaaacagc
     5281 gcggccgccg cgaccgcggc gatccactcg ccaaggctga ccctggatgt gtccacgact
     5341 ccccctccac ctgtttgcag ggatttcgcg cgggactgta gttacccctg gggacggctc
     5401 tcaatcggct cttcgtccgg caccgcccgc gatactgccg ggccgtggac gcgatgaggg
     5461 agatcgacgc gctcgtggag ttccgcggcc gcgctgccgg caccgacgcc gagcggcgcg
     5521 cggcggagca cctcgcgaat cgcctacgcg agatcggccg cgacgccgac gtcgagccgg
     5581 tgcacgtcta tcccaactat ccggtcaccc acgcgatcca cgcgctgctc gcgatcgcgg
     5641 gcagcgtgct gtcggtgtcg cacccggcgg tcggggcggt actggtcctg gtcgcgatgc
     5701 tctccgcgct cggcgacctc accggcgtgt tctacgccgt gcgccgcctc actgggaggc
     5761 gcgcgtcgca gaacgtgttc tcgcgcgtgg acgccggccg cgacggcacg ctcgtgctcg
     5821 tggcccacta cgactccgcg cacagcggcg cggtgttcgg gggccgtgtt caggagcgcc
     5881 gagcggtgct gggcaagctg ctcaagcggc cgatcggttt gttcgagccg ttcttctggt
     5941 cgatcgtcgc ggtgctcgcg tgcacgatcc tccgggtcgc gggcatcgac gcgctcctgc
     6001 tcacgatcgt gcagttcatc ttcacggtcg tgctgatcgt gagcgtgccg ctgctcctcg
     6061 acatcgcgct ctccgacccc gtgcccggcg ctaacgacaa cgcctccggt gtggcaaccg
     6121 tgctgcggct cgccgagcgc ttcggctacg tgctcgacca cttccaggtg tgcgtgctct
     6181 tcacgggcgc cgaggagggc ctgctcggcg ggatgcgcga gtggatgcgc cgccaccgca
     6241 agcagctcgc ccccgagcgc accgtgttcc tcaacgtgga caaggttggc accggcaccg
     6301 tgcgtttcac caccaaggag ggactggtga tcgcctcgcc ctaccacccc acgctgatct
     6361 cgctgtgcca tgagatcgcg gacgaggacg acgagggcgg ccgctacggc gcgcgagggc
     6421 tcgtgtcgcg gcacgtgacc gacgcctacg ccgcgcgcac cgccggcttc ccggcgatca
     6481 ccatctcgtg cgcaaacgcg ctcgactacg tgcccgagta ccaccagcac agcgacacga
     6541 tcgatcacat cgactccgag gcgctagacc gcgcctacgg attctgctgc gagctgatcg
     6601 agcggctcga cgctcgcgtt ggcgccgacc tcgccgccgc gcgatccgac caagagacgg
     6661 cgctgtcgga ggaggagacc gtctagtcgt cgctgccacg ggcgcggccg cgactgacgc
     6721 gccacgtagg aggcaccggg cgccgccgcg gctccgtctg gaggactccg cggcggctac
     6781 ccgctgccgg gccccggcca ctgcgccgat ttgcggccgg ggccgtccgg gcttggatgg
     6841 cccgtggctt gagggacgag ggctcctggg gatgagccgc tgcgtcgatt cgcgaagcta
     6901 cgcccggcgc gtaaagccgg actaaaagcg agattcagct tctgggccgg attgtcagcg
     6961 ccttggcggg agccgccgtg ctcgtgcccg agcgctcgcc cgcctcgcgc gtgagcagct
     7021 tgaacgtaag cctcggcttc cggtggcgct ggaaggcact tagcagggac ttcgccggct
     7081 tgagcgctcc accgatgacg atcacgcgca ggctctgcgt gtccttcggc agacccgtga
     7141 gcacgaggcg tgagccgcga agcgtcgcct tcatgcccct cacgcgcttg ctgccgctga
     7201 ggaccctgac gccgcgcttc gctttgcgcg agacgccctt cagcgagcgc ggcatgcgca
     7261 gctcgaggcg ctcgaagccc gccgagttcg ggaagccgcg cgtgcgcagg tcgaggcgcg
     7321 gcttcttctt cttgacgccg ctgagcgtga gcgaggcctt cgtggcgcag cccgacacga
     7381 gcagcggcga ggtgaccgtc ctcgtcgcgc cgctgtgggc ggtgaaggcc gcgctcacct
     7441 tctgcgtgct ggcgcagagg tcgcgcacgg cgacgagaga gcccttcggc ccgccgtcga
     7501 aggcgagctc gaatttgccg atcggcacgt cgggcaccga atcgaagacc gtcacgagtc
     7561 ctttctgagt gccggtcaca cgcccgttga gcttcaggtc gagaaggccc ttgaggtcga
     7621 gcaggacgcc ggggaacgcc tgcccgggga tctgcacgag gtagaccggg cccgcgagcg
     7681 gcagcggcaa cagcggcgag ctcgccgtag ccgtgccgac cttcgcgctc ggcgggcagg
     7741 tcgccgtcgc ggcgaccgct tgcggacagg cgcgacccac tgcctcgagg tcagggccaa
     7801 tcgtcggcgg cagcgtgacc gccacgctct tctgcgcggc ctcgccctcc ttctgcgtga
     7861 tcaccgtccg gaggaacggg tgcctcagct ggttcgtctc gccgcggccg ccggcgacgg
     7921 cgctgaagcc gggattgaag ggcagctttt cgcagcccgt cgcctggaac ggcgcggtcg
     7981 ggtcggcggt gtcgccctct gcggacgtga agtgcccgac cacctggagc ggcgcgcagg
     8041 aggtcgggtt cagcagcatg cccggccgat cgagcttcac cgacagatgc cggatcgccg
     8101 tggggatgcc accgacgaac tgcggcaggt cgtcggaggt gacgtcgatg cccccgtcgg
     8161 tgccgcgcag cttcagggcc gagcgcagca cggtggtgcc cacgtcgagc gggccgacct
     8221 tgccaggcag cacgacgatc aggctcgcga tccctccgtt gtacggcccg ccgaagtaaa
     8281 cggggccgct gagcggcgtg ggcgcaccgc ccgacccaac cgcagccgtc accgtgccca
     8341 cgcgcgagtt ggcggggcag ttgccggtgg ccgcatcggc gttgccgcag agcggcacgc
     8401 ccgcaacctt gcccaggagc ccgggcgcga gcgagaactt gaggcccttc agccgctgat
     8461 cgcggtcggg gcggtcgacc accaggtcga gcgagccggg gtcgtggccg gcctgcgtgt
     8521 tgcctggaat cgcctggagc gtcggcgtga acggccgctt cgccgggcag gcgccaccgg
     8581 cgccgtcgtc gtcgaccggg aacggggcgc tcgtgggcgt cgcgtcgggc tgaccgctcc
     8641 atggagtgac gcgcgcggtc gccgtgtagc tcccgcactc gaccgggttg gcgaggatcg
     8701 cgcggtcgcc gccccggaag ctcagtgcga acgttgtgaa cggggtttgc gggaggtcat
     8761 cgaagacggt cgttacctgc ccatcggcgg ggttcggcgt gaccagaccg atgactttga
     8821 tgctcagtcc gtgttcctga acgccaacga agaggcgcag cggggcgtcg gccgtgggcg
     8881 tcccgagcca cacctttcca ttgagcaccc cgagaagcgg cgtgttgata gtgacggtgc
     8941 ctaattcgga gttgcccggg cacgcaacgg tatcggcggt cgccttcgcg aactgcccgt
     9001 ccgtgcacgc ggacatgcct tcggccgagg gcggtgagag cgtcgtgccc ctcggaagga
     9061 ccacttcggt gcgcttgacg tgcgactggc cgcccggcac gttcactgcg atcgtgtacg
     9121 cggtgggggt gtctgcgcgc gagctcgcac gcgtgacgct cagcgtcggc gcgaatgtcg
     9181 gaaggctgga acagccggtc acgttgtagg tcgcagagtt ggagctgaag ctcgctggcg
     9241 ccgcgtacga cgcggcgtcg acggttgtgg tcgcggcgcc gcagcgggtg ggattcgtca
     9301 tgaacggtcc cgagccgccc gccggggtgc cgtcgagcgt tatgtccatc tgcttgacgt
     9361 tcagcgacac cccgccagga gccgtgcgtg ggatctcgtc aatcgtcgtg tcgagtccgt
     9421 agtcagtcgg acggacggct acctcgaccg gaatcttcag ggtgaagccc gggatcccgc
     9481 caaggggaag cgagttcacg atcgcgccga ggcgagcagg ctcgtccggt cccggctcga
     9541 cgttgtagat ctttcccgac gccgtaatcg gcacgggccc gatgagccct agcaagacaa
     9601 aggcgtcagc actcacgctg gtggtaccga cagccgtatc actcgggcaa ctgcccgcgt
     9661 caaagctcgt ttgctggcat ttggccgtag ccgatggatt cccaacgagg ccgggcggca
     9721 gatggagacg cagtgtcttg acctgatcct gcggatcact gaactggatc gaaatcggaa
     9781 cgtccggatg agccccggct gccccggatg acgggttgac cgtgaatgtg ttgacgccga
     9841 aagcggcgtg cgccggagcg gcgaccagca agagcgtcgt gatgctcgcc gcgaacgcgg
     9901 caagcgggcg agtccggttc agtgcgagtc tccttgtttc aggctcatcg cccatccatg
     9961 agcgacggcg cctcccagcg cgagcgggtt tctacccatc tgaccggtcg gttagtcctg
    10021 acgcccgcgt ctcgtggggc atccgcgctc gaccccacgg ggtgtgacgc acctttcgtt
    10081 ccgatcgttg atcggttcaa cgatcggaac ggacaggtgc gaagcgtgct cgtccgcggc
    10141 cggtggccac gccgccctcg cgatcgcatc gttgatcgat tcaacgatcg gaacggacag
    10201 gagcgacccc ggtcatcgcc acgccgccac ggccgcgccg gccacggcgg cgcccgctcg
    10261 cggagttgat cgttgatcga ttcaacgatc aaaacggacg ttccgtgccg atcgcggggc
    10321 tgttaggggc gagttctcgc cccgcccgta cttccaggga ttgcttccac tgttcaacta
    10381 tcgaaaagcg agagcactca tgccgaagcg aattcccccg ggccccctcg ataacgaaat
    10441 gccgccggtg ctcgcggaca gcgcagccct cgccctgact cgtgcaggag cgctagctgg
    10501 cgagaagctc gagcgcgtct tccgccgcct cgggtgtcac gtccgcgact acgccgtcct
    10561 cgagcttctt gccgcgaccg atccagggcc gatctacgac gggccggcgc gcctgttcgg
    10621 cgtacgcatc ggcatggcgc agtcgcggct gcgaaagcgc atcggtctct cccccgccgc
    10681 tctttcccgg ctgttgcgcg agatggagac cgccggcttc atcaacctcg gctttggaag
    10741 ggacggacgg gagcggttcg tcgagctgaa gccgctcggc aaggagcgtc tcacgccgtg
    10801 ccgcgaggcg ttgcgcgcat ctgaggagga gatcttcgag ggcatcgatg ccgatcgacg
    10861 cgagcagctg cgcgaaacgt gcctccagct tccgccgctc ggtggccgtg accgcagaga
    10921 ggagtggtgg ctcagacggg gctgatcggg ttgttcttgg gcggccacac ccgacgccgc
    10981 gaggcgatca gctcgtagcg gcgcaccaag tcggtgcgga gatcctcagg ctgcacgacc
    11041 gcgtccacca ctagttcgga ggcgaggtgc aggatgtcga tctcctcggc gtactcctcg
    11101 cggagcttgg cgacgaaggc ctcgcgctcg tcgggatcct cgatcgcctg gacctggttg
    11161 tagtagaccg cgttgatcgc ggcctgtggc cccatcaccg cgatcttggc ccagggcagc
    11221 gcgatgcacg cgtccggttc gaaggcgggg ccggccatgg catacaaccc cgcgccgtag
    11281 gccttccgca cgatcaccga gatcttcggc acggtcgcct cggacacggc cgagatcatc
    11341 ttggcgcccg cgcggatgat gccctgcttc tcgacctggg ttccgatcat gaagcccggg
    11401 acatcggcca aaaacagcag cgggatgttg aaggcgttgc atgtccagat gaagcgagca
    11461 gccttgtccg aggaatccac gaacagcact cccccgcgct tcttcggctg gttcgcaacg
    11521 atgccgatcg ctcgcccgtc cagccgcgcg tacccgacca gcagctcctt cgcccagcgc
    11581 ttctgcacct cgaagaacga gccctggtcc acgaggccgt cgatcagctg gcccatatcg
    11641 aatgccttgt tctcatcggc gggaaccagg tccgcgaccg gcaccgcgtc cgcgggcggc
    11701 ggagcgggtg gcgcgtgcgg cggctgctcg cgccagttcg agggcatgaa ccccagatac
    11761 cgcttcgcgg tctcgatcgc ctcctcgtcg ctctttgcca ggaagtggcc gacccccgac
    11821 acgccggtgt gcatgcgcgc gccgcccatc tcctcgagcg acaccttctc gccgatcacc
    11881 atctccgcca tacgcggcga gccgagatac atggaagcgt tgccgtcgcg catgatcacg
    11941 acgtcgcaga acgccgggat gtacgcgccg ccggcagccg aaggcccgaa cagcacgcag
    12001 acctgcggca cgacgccaga aagcttcacc tgcgtatgga aaatgcgccc cgcgccgcgg
    12061 cggccgggga acatctgcac ctgctcggtg atgcgcgcgc ccgcggagtc gacgaggtag
    12121 atcatcggca cctcgtggag cagcgcctgc tcctggatgc ggatgatctt ctccacggtc
    12181 ttcggccccc atgagccggc cttgacggtg gggtcgttcg ccatcaggca gacggcgcgc
    12241 ccgcccacgc gggccatgcc ggtcacgacc ccgtcggccc cgaagccctc ctcctcgtgg
    12301 tgcgcgagaa gcgcctcttc tgcgaacgaa tccgcgtcga tcagtcgccc gatccgctcg
    12361 cgtaccggca gcttcccctg ctccgcggcc ttcgagcgat gccgctcagg cccgcctccg
    12421 agggcgcgct cgagagcttc tcgcaggtcc gacacgcgcc tacagccccg ggtcgaccgc
    12481 tacgggcttc tcgcccgagt agtcgtagaa gccgcgtccc gacttccgtc cgtaccaacc
    12541 cgccgacacc atcttccgca gcgtcggcgg cgccgcgaag cgacgctcgc ggtactcgtc
    12601 gaacatgatg tccgcgatcg acttcagcgt gtcgaggccc acgaagtcgg acagcagcag
    12661 cggccccatg ggatgtccgg cgccggcctt catcgcggcg tcgatttcct cgatcggcgc
    12721 gacgccctcc tcataggcgc ggatcgcgtc gagcatgtac ggcacgagca gccggttcac
    12781 gatgaagccc gtcttgtcct tcgcgggcac ggtcagcttg ccgagcttct cgccgaaggc
    12841 gaacccggcc gcgagcgact catcgtcggt cgtcacggtc ttgaccacct cgagcagcgc
    12901 catctgctgc acggggttga agaagtggag tcccacgaac cgctccggcc ggcgcgtggc
    12961 cgcagcctgg tcgatcaccg acagcgaaga cgtgttggtg gcgagcaccg cgccgtcctt
    13021 gacgatcggg tcgagctcgc gccacatttc gagcttcaac ccgaggtcct cggtgatcgc
    13081 ctcgaccacg aggtcggagt cggcgagatc gttgtaatcg gtcgtgccgt ggatgcgtcc
    13141 gagcaccgcg tcggcctccg cctgctccat gcggcccttc tcgaccgcgc gcgccagctg
    13201 cttctgaatc cgtccgagcc ccttgtcgag ccgctcctgg tcgacctcgc gcaggaccac
    13261 gtcgtagccg gactgggccg ccacctgtgc aatgccatgc cccatcagac cggctccgag
    13321 cacgcccacc ttcgagatct ccattagcct ccgtctcctt gaccgattcg catttcgagc
    13381 gccgggagcc tacatgggac tcgtagccgt cgaggaccgc gacgcggtcc gccacgtggt
    13441 gatggagcgc gctgagaagc gcaacgccct caacgacgag ctcgtgctcg cgctgggcga
    13501 ggccttcgag gaggcggcgg gcgacgactc ggtgcgagtc gtagtgctgc gcggcgacgg
    13561 accgatgttc tcctccggca tggacctctc tggcctgcgc gacctctccg aaaaccccga
    13621 cggcctgcgc gagttccgtg cgccgttgat ccgcatctac aacatgctcg aggagatgcc
    13681 gaaggccacg atcgcccaga tccacggcgc ctgcatcggc ggcgccatgg agctggcgct
    13741 ggcgtgcgac atgcgcacca tggccgagga cgccgtgtgc ggcctgctcg agacgcgggt
    13801 cgggctgatc cccgacctcg gcggctgctc gcgcctgccc tccgttgtcg gcgcgggcat
    13861 cgcgaaggag ctgatcatga ctggcaaggt gatcgacgga cgcgaggcgc accggatcgg
    13921 cttcgcgaac cgcatcgcgc ccgccgaaga gctcgacgcg gccaccgatc agctcgtgaa
    13981 cgagctgctc gccgctgcgc cgaaggccgt cgcgctcgcc aagcgcgtga tcgaccaggc
    14041 cgccaagccc gacctcgagc gctcgctcga gcgcgaggag gacgcgcagg ccgagctcgc
    14101 cgcatccgcc gacttcgccg agggcacgaa ggcgttcttc gagaagcgcc agcccgagtt
    14161 cgcgggccgc taggccttcg acagctccgc caccagctcc tcgagcgggg tctccacgtc
    14221 ggcgagccgc gccgggtcca tgcggcccca cgagcgcacc agttcctgga tcgggtcggt
    14281 cacgtcccag acgttcatgt tcagcccggc gatgacgctg ccgtccttga gccagaacgc
    14341 gatcagctcg cgcgtctcct ggtcgccgcg gatgacgagc tcgtcccacg gcgtaggcca
    14401 gcccgagtac tccatgccga cgtcgtactg gtccgagaag aagtagggca cgcggtcgta
    14461 ggacaccggc tggccgagca tcgcgcgcgc cgccgccggg ccctggttca gcgcgttcgc
    14521 ccagtgctcc acgcgcacgc gcgcgcccgt gatgggatgc agctcgttcg caacgtcgcc
    14581 cgccgcgaac acattcggca cgctcgtacg aagggaggca tccacgagga tgccgccgtc
    14641 cccgacatcg atcccggctg cctcggcaat cccgatgcgc ggaaccgctc cgacgccgat
    14701 gagcacgaga tcgcagtcga gtgcgcgacc gtcgctcgtg atcacgcgct ccgcgcggcc
    14761 gctgccttcg atccgctcga tcccggtctc catcaggacc tcgacaccgt gctcgcgatg
    14821 cacgtccgcg tagatcccgc cgacctcctc gccgagcacg cgctcgagcg gcgtacgcag
    14881 cgggtcgagc agcgccactt ccatacccat ctgccgcgcc gaagccgaca cctcgcaacc
    14941 gatccagccc gcgcccacgg cgaccaggcg accgccgcgg ctgaagagct cgcgcagctc
    15001 gtctgagtcc tcgacgtcgc gcaggtagtg cacgccgtcg agggtcatgc ccgggatgtt
    15061 caggcggcgt gggctcgagc cggtggagat gagcgcagcg tcgtacgaga tcctctcgcc
    15121 ggagtcgagc acgacctcgg atgcaccggt gtcgagcgcc gcggcggaag tgcgcgggcg
    15181 caggtcgatc gcgtgctcct cgtagaagcc gggctcgtgc gcgtacacct tgtcgcggcc
    15241 cgcctccccg cgcaggtact ccttcgacaa gggtggccgc tcgtacggtc gctcgtgctc
    15301 ggcatcgagc agcagcagct cgccgtcgaa gccctcctcg cggagcgtct ccgcggcctt
    15361 cgcgccggcg aggccggcgc ccacgatcac gaacctgcgg gtggttgaca tggcgtcctc
    15421 cttacacggg gtggggactg cagcccgcga gcgcaagaaa ctcttgtctg gtgcggggat
    15481 cgtcgcgcac gagcccgtag agcgcggacg taaccgtgcg cgtgccgggc ttttgcacac
    15541 cgcgcagcgt catgcacatg tgctcggcct cgagcaccac gcccacgccc ttcgggtgaa
    15601 gcgcgtcctc gagccagccg gcgatctgct tggtgagacg ttcctgcacc tgcaggccgc
    15661 gggcgaacat gtcgaccact cgggcgagct tcgagagccc gaggatccgg tcgcccggca
    15721 ggtacgccac gtgtgcgacg ccgtggaacg gcagcaggtg gtgcatgcag agggagtgga
    15781 acgggatagc gcgcgcgagc acgagctcgt cgtagccctc ctcgttctcg aaggtcgtca
    15841 ggttgaacgg ctgcggggtg agcagctcgg cgtaggtgcg cgccacgcgc cgcggggtgt
    15901 cgcgcagccc gtcgtggtcg aggtctgcgc ctagcgcctc gagcagatcg cgcgccgcgc
    15961 gctcggccgc atcgacgtcg atcgacgtcg agtcgccggt gacgacgtga aggttcggac
    16021 gctcggtcat ctagagagct tcgcctcgca tctacgacgc tctgggtcgt gtgagctttc
    16081 cacgacgcgt gcgccgctgc cctcgaccac gccgagcgcg acaccgcaca ggagttcggg
    16141 gtggtccggc agcacgagcg ggcacgggca ggcgctcaac cggagcgtgt cgcccgtgat
    16201 ccgggcgtcg aagccgagcc gctcgagcgc gcgcgggaga tccccgcagc gacagcccca
    16261 ttcgcgcgcc gccgcctcga ccggctcgtt tcgatcgacc gccatcgcaa gcacctcggc
    16321 aaggccgcgg aaatcggccg ttggaagctc atagccctcg gcgaggcggt acctgatcgg
    16381 cggcctgccg cgccccgccg gggtggcgcg ttctccctcg atcaacccag cgcgctcgag
    16441 cgccgcgaca tgcggtttgg cggtgttcag gtgcacgccg gccgcgtcag caagctcctg
    16501 gagagtcgag ccgggtcgct cgccgaggtg atcgaggatg cggcgccggg ccgggtcggc
    16561 aactgattcg aaagtcgtcg gcgacatcgg tggccatatt aaccgacaaa ttcttgtttg
    16621 taaatacgtg cgcgaatgcg ctcggcgggc aacttggctc acagcccggc gaaggcgcgg
    16681 gcgatcccct ccgccagccg taggcaggaa tccaccgagc gccagtccac ccgctcgggc
    16741 gtgtcgtcta gacggtggta cggcccgaag ccctccggcc cgatggaaag catcaagatc
    16801 gcgggcaacc cggcgtacac cgcgaggatc gggtcggtcc acgcgccgag ccgcaaacgc
    16861 cgcggttgct ccgtacccgc acgctcggcc ccaaggtcgg cgagcgccag gtcctcggct
    16921 cggtagtgga ccgtgtgcgg cagcgcctcc cccgtgagca cgcatggctc accggccgcg
    16981 agcgtgtcca ggccgagcac gagcgtcgcc cccggatcca gccggccgcg gttctgcgcg
    17041 agccagtggg ccatgccgcc cattcccgct tcctcgcagc ccgggaagag caatgtgacg
    17101 tcggtgccgg gcagcggctc ggccgcgaac cgcccggcca gcgcgagcac ggcggcgacg
    17161 ccggtcgcat tgtcgttcgc accgggcacg acagcgccgc gcgccgcgcc cgcagcgagc
    17221 gccgagccga gcgcgagcac cgcacctccg agcgcccggc cggcgcgtgt gccggtcgca
    17281 accgcggcga gcgcaagcga tggcggcagc gcgaacgaat cgacccggcc gcgatcgagc
    17341 gcgtgccgga agctgtggcg cacgagcgcc gggtgccaca ttaacccgga tcgcgccgcg
    17401 tcgtggtggg cgaccagcac caggtggcga cggggctcgc cggccgccgg cagggtcgcg
    17461 acgacgttcg tgccctcgcc cgccggcatc acgcgcgcga gccactgcgc tcggccgctg
    17521 aactcgagcg cgaacgacgc gagcgtcccg agcgccaagg tgcggctctt caacgctgca
    17581 gcgagtacac ccgccgcgaa atgcggcagc tggcgatcgg cccagctcgg ctggtagcga
    17641 aagctcgcaa gccgaacgtc gtgcgcgccg atccgctcga gccgccccgc gatccactcg
    17701 gccgactgcc gctcgcccgc ggtcgccgag ccccgggcga tggcgcagag cgcctcgatg
    17761 tccgtgcgcg tcacccgcgg tgccagtcca cagggccggc cttcagcagg tggccggtga
    17821 gcgtgcgccc gagcacctcc tgcaccgcgc gcgtcgcggc cagcagcttc tcgagatcga
    17881 ctcccgtgta gtagcccatc tcgtggagca tcgacacgag gtcctcggtg gcgatgttgc
    17941 ccgtcgcgcc cttcggcacc gggcagcccc cgagctcgcc gaacgacgac tcgaacgagc
    18001 gcacgccctc gtcgagcgcc gcgaccacgt tcgcgaggcc ctgcccccgc gtgttgtgga
    18061 agtgcgcggt gagctgcacg ccgggcagcc ggttggccgc ggccgcgaag aacgcgcgca
    18121 cctgccgcgg gttagccatg ccggtcgtgt cgccgaaggc gatctcctca gcgcccgcgg
    18181 ccaccagccg ctcggcgatc ccgaacacgc gctccggcgg cacctcaccc tcgtaggggc
    18241 agccgaagct caccgagatc acgccctcgc agcgaagtcc gtccgcacgc gcatgcgcga
    18301 tcacgcgctc gagccccgag agcgactcct cgatcgagcg gttcacgttc ttctggttgt
    18361 gcgtctcgga ggcggacagg aagaggttga cctcgtcgaa gcggtcgcgc agctcgagcg
    18421 ctttctcgag cccgcgctcg ttcgggatga ggaccgtgac cgccacctcg cgtgggatgt
    18481 cgaccgcccc gagcacctcg cgcgcgtcgg acagctgcgg gatcacgtcg gggcgcacga
    18541 agctcgtgac ctcgaggcgc ggcacacccg tgcgcgcgag catgtcgatc agccgcacct
    18601 tgtcggccgt cgcgatcacc tcgggctcgt tctggaaccc gtcgcgggga ccgacctcgc
    18661 gcactttcac ctgctcggcc actggagccg gaaacgctat cggtagggtg ccccgccgtg
    18721 gacgagccag taccg
//
LOCUS       DASVXS010000229         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_558727, whole genome shotgun sequence.
ACCESSION   DASVXS010000229 DASVXS010000000
VERSION     DASVXS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558727"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VF752_12920"
     CDS             complement(<1..1075)
                     /locus_tag="VF752_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX8856490.1"
                     /translation="MSEGDRGPHLYPVPGVGDSADGAAAAAAAEVPEIGNGITPPRHR
                     GRRGRFISDVIVELGFLPKERVDAAVEEARGSGRTPEQVLLESGGLTADQLARATAER
                     FGLDHVDLTIYKPDVGALNLVNVQSARRYNAAPIGFDDGVLLVAMADPSNVLALDDLK
                     LLTKQEIRPVVASAEDIAALISKMNRLDDAVAEAISEDDSEELALVSDIRESADDAPV
                     IKLVNSIIAQAIEEGASDIHFEPDGREMRVRFRVDGVLSESTTIPKRMLSGVISRVKI
                     MANLDIAERRVPQDGRVSLTVEGHGVDVRVVTLPSVHGEGIVMRILDKEQVLLTLDTL
                     GMRDEARERFEAGFKQSYGAVLVT"
     gene            complement(1072..1503)
                     /locus_tag="VF752_12925"
     CDS             complement(1072..1503)
                     /locus_tag="VF752_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856491.1"
                     /translation="MHKATPAKPAAKAETKANAKTTPAHAPAAKAPAEAPGASSGLPA
                     SVAQAFAQHKIVVLFFEGRGADDEATAQSVKALKSASHGKVAVFSDKLSNLADYRRIV
                     EGLDISQAPSTVVVGRDMKARLIEGFVDSGSLRQVVADVAG"
     gene            complement(1715..2491)
                     /gene="aroE"
                     /locus_tag="VF752_12930"
     CDS             complement(1715..2491)
                     /gene="aroE"
                     /locus_tag="VF752_12930"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX8856492.1"
                     /translation="MLAGVLGFPVAHSRSPQMHNAAFEALGLDWRYVRLPVSSELFDE
                     TVRALPGSGYGGANVTIPHKQAALALADERSDAAAAIGAANTLTFAEGGIRADNTDAG
                     GLLDALGESPAGQRALVLGAGGAGRAAAYALREAGAEVAVWNRTAERARALAADLDVD
                     AVQTTEPAAFDLLVNTTSVGLDGQGGLGELGLAGAAPPATVVDLVYGERETPLCAWAR
                     EGGARVVDGLEVLVRQGALSFHIWTGAEPPLDVMRAAARR"
     gene            complement(2491..3714)
                     /gene="mltG"
                     /locus_tag="VF752_12935"
     CDS             complement(2491..3714)
                     /gene="mltG"
                     /locus_tag="VF752_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX8856493.1"
                     /translation="MPGGRSESEREAARSEREARRREAWGEPMPDFDFEHANGEGGAP
                     PGRPRRRVGRGPGRPVRVRRGRVAALAALLAGLAVVLWFLVSLFQPFKGDGSGSVRVV
                     VPKGAGLGEIATALDKRGVISSTFFFKLRARIDGRTSDFKPGTYTLKHDMKYTAAIDA
                     LVKGATPGIVNVTIPEGRARSEIAPIVKSAGLRGSYLTVSKRSPELNPRRYGAKSAAS
                     LEGFLFPSTYELKPHSSTRDLVSKQLATFKQEFGQVNLSYARKKNLTAYDVLTIASMV
                     EREAQLDKERPLIAAVIYNRLRQGMPLGIDATIRYVTGNWTRPLKQSELAIRSGYNTR
                     TNTGLPPGPIGNPGLKSIEAAARPAKVSYLFYVVKPGTCGEHAFSSTDAQFQRDVAKY
                     NNARNAKGGKSPTTC"
     gene            complement(3735..4130)
                     /gene="ruvX"
                     /locus_tag="VF752_12940"
     CDS             complement(3735..4130)
                     /gene="ruvX"
                     /locus_tag="VF752_12940"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX8856494.1"
                     /translation="MSRVLALDYGEARCGAAVSDPTGTLATPLSAVERPATRRGLDRV
                     VALVRAQGAERVVVGLPLTLSGEEGPQAEETRRFAERLAERVGVPVDLHDERLTTRQA
                     ERSGGRADADSRAAAVLLESYLARLERSR"
     gene            complement(4127..>4994)
                     /locus_tag="VF752_12945"
     CDS             complement(4127..>4994)
                     /locus_tag="VF752_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX8856495.1"
                     /translation="GSYVGPDKLRFDFTHGERLTPDQLAAVTDLVNEWIVATHPVRAI
                     ETTRDEAERLGAMALFGEKYGDWVRMVEIEGVSRELCGGTHVASSAEIGLFHLTHETS
                     SAANVRRVEAVTGAGGIDVFRERTEEMRRLAAMLRVPERDVVSAVDKLQEQLREAQRR
                     PRQDDRQLAQGLAEKAEEIGGVRVLAQVVEVPDRDTLLQLSDQLKQTLGDSAVVLGTA
                     IDGRVHLIANFSPGAVERGLKAGDVVRAAAEVAGGGGGGRDNMAQAGGRDPEKLPEAI
                     AAARLAIEKTLR"
BASE COUNT          682 a         1821 c         1721 g          770 t
ORIGIN      
        1 ccgtgaccag caccgcgcca taggactgct tgaagccggc ctcgaatcgc tcgcgcgcct
       61 cgtcgcgcat gccgagcgtg tcgagcgtga gcagcacctg ctccttgtcg aggattcgca
      121 tgacgatgcc ctcgccgtgc accgacggca gcgtcacgac gcggacgtcc accccgtgcc
      181 cctcgaccgt gagcgagacg cgaccgtcct gcgggacgcg ccgctcggcg atgtcgaggt
      241 tcgccatgat cttcacgcgc gagatgacgc cggagagcat ccgcttgggg atcgtggtcg
      301 actcggacag cacgccgtcc acgcggaagc gcacgcgcat ctcgcggcca tcgggctcga
      361 agtggatgtc ggacgcaccc tcttcgatcg cctgggcgat gatcgagttg accagcttga
      421 tgaccggcgc gtcgtccgcc gactcgcgga tgtcggagac gagcgcgagc tcctcggagt
      481 cgtcctccga gatcgcctcg gcgacggcgt cgtcgaggcg gttcatcttg ctgatcaggg
      541 cggcgatgtc ctcggccgag gcgacgaccg gccggatctc ctgcttggtg agcagcttca
      601 ggtcgtcgag cgcgagcacg ttggacgggt ccgccatcgc cacgagcagc acgccgtcgt
      661 cgaagccgat cggcgcggcg ttgtagcggc gcgccgactg cacgttgacg aggttcagcg
      721 cgccgacgtc gggcttgtag atcgtgaggt cgacgtggtc gagcccgaag cgctcggccg
      781 tcgcgcgcgc gagctggtcg gccgtgaggc cgcccgactc gagtagcacc tgctcgggcg
      841 tgcggccgga ccctcgtgcc tcctcgaccg cggcgtccac ccgctccttg ggcaggaagc
      901 ccagctcgac gatgacgtcg gagatgaagc gcccgcgccg gccgcggtga cgcggcggcg
      961 tgattccgtt cccgatctcc ggcacctcgg cggctgcggc agcggctgcg ccgtcggcgg
     1021 agtcgcctac gccgggaacg gggtagagat gaggcccgcg gtcgccttcg ctcatccggc
     1081 cacgtccgcc accacctggc ggagtgaccc ggaatcgacg aagccttcga tcagccgtgc
     1141 cttcatgtca cgaccgacga cgaccgtaga tggagcttgc gagatgtcga gaccttcgac
     1201 gatccgccgg tagtcggcga ggttggagag tttgtcgctg aacacggcga ccttgccgtg
     1261 acttgccgac ttgagcgcct tcacggattg agccgtggct tcgtcgtcgg cgccgcgccc
     1321 ttcgaagaag agcacgacga tcttgtgctg ggcgaacgcc tgagcaaccg aagcgggcag
     1381 gccactcgac gcaccgggtg cctcggccgg agccttggca gcgggggcat gcgccggagt
     1441 cgtcttggcg ttcgccttcg tctcagcctt ggccgcgggc tttgcgggcg tcgccttgtg
     1501 caccgccggg ttctgcgcct tcggtgcggg actgggcttg gcagcctgag gtgccggcgc
     1561 cggcgtggag ccgccatccg acgagctgtg cctggtcgcg acgaatgtcg ccgagatcag
     1621 gagaaagccc agaactgcga gtagaacggg gcgaggaacg gacatcgccc ctcctatcgg
     1681 caggcgatgg acggatgttt agcccctctg gacgctatcg acgcgcagcg gctctcatca
     1741 cgtcgagcgg cggctcggcg cccgtccaaa tgtggaagct gagcgccccc tgccgcacca
     1801 gcacctcgag gccgtccacc accctcgccc cgccctcccg cgcccaggcg cagagcggcg
     1861 tttcgcgctc tccatagacg agatccacga ccgtcgcggg cggcgccgcg cccgccagcc
     1921 ccagctcccc gaggccgccc tgaccgtcga gtccgacgga ggtcgtgttc acgagcaggt
     1981 cgaaggcggc cggctcagtc gtctggacag cgtccacgtc gaggtccgcc gcgagcgccc
     2041 gagctcgctc cgcggtccgg ttccacaccg ccacctcggc ccccgcctcc cggagcgcgt
     2101 aggccgccgc gcgtcccgcg ccgcccgcgc cgagtacgag cgcccgctgc ccggcgggcg
     2161 actcgccgag cgcgtcgagc agcccgccgg catcggtgtt gtcggcgcgg atgccgccct
     2221 cggcgaacgt gagcgtgttc gcggcaccga tcgccgcggc ggcgtcgctg cgctcatccg
     2281 cgagcgcgag cgcggcctgc ttgtgcggaa tcgtgacgtt ggctccgccg tagcctgagc
     2341 cgggaagcgc gcgtacggtc tcgtcgaaca gctcggacga gaccggcagg cgcacgtagc
     2401 gccagtcgag gccgagcgcc tcgaaggcag cgttgtgcat ctgcggcgag cggctgtgcg
     2461 cgaccgggaa gccgaggacg ccggccagca tcagcaggtg gtcggggact tcccgccctt
     2521 cgcgttacgc gcgttgttgt acttggccac gtcgcgctgg aactgagcat cggtcgacga
     2581 gaaggcgtgc tcgccgcacg tgcccggctt caccacgtag aagaggtagc tcaccttcgc
     2641 cggccgcgcc gccgcctcga tcgacttgag gccggggttg ccgatcggcc ccggcggcag
     2701 gcccgtgttc gtgcgcgtgt tgtagcccga gcggatcgcc agctcagact gcttgagcgg
     2761 ccgcgtccag ttgcccgtga cataccggat cgtcgcgtcg atccctagcg gcatgccctg
     2821 cctgagccgg ttgtagatca ccgcagcgat gagcggccgc tccttgtcga gctgcgcctc
     2881 gcgctccacc atcgacgcga tcgtcagcac gtcgtatgcc gtgaggttct tcttgcgcgc
     2941 gtacgagagg ttgacctggc cgaactcctg cttgaaggtg gcgagctgct tggacacgag
     3001 gtcccgtgtg ctcgagtgag gcttcagctc gtaggtcgag gggaacagga agccctcgag
     3061 gctcgcggcg ctcttcgctc catagcggcg cgggttgagt tccggcgagc gcttgctcac
     3121 cgtgaggtag ctgccgcgca ggccggccga cttcacgatc ggcgcgatct ccgagcgcgc
     3181 gcggccctcc gggatcgtga cgttcacgat gcccggcgtc gcgcccttca cgagcgcgtc
     3241 gatcgcggcc gtgtacttca tgtcgtgctt gagcgtgtag gtgccgggct tgaagtcact
     3301 cgtgcgcccg tcgatccggg cgcgcagctt gaagaaaaac gtgctcgaga tcacgccgcg
     3361 cttgtcgagc gcggtcgcga tctcgccgag gcccgcaccc ttcggcacca ccacgcggac
     3421 gctgcccgag ccgtcgccct tgaacggctg gaagagcgag acgaggaacc agagcacgac
     3481 cgcgaggccc gcaagcagtg ccgcgagcgc cgccacgcgg ccgcgacgca ccctcaccgg
     3541 tcgccccggg cctcggccga cgcggcgccg agggcggcct gggggcgcgc cgccctcccc
     3601 gttggcgtgc tcgaagtcga agtcgggcat cggctcgccc cacgcctcgc ggcgcctcgc
     3661 ctctcgttcg ctgcgcgcgg cctcgcgctc gctctccgac cggccacctg gcacgggagg
     3721 aggcccgtag ccgctcaccg agatcgctcc agcctcgcca ggtagctctc cagcagcacc
     3781 gccgcggccc gcgagtccgc gtcggcgcgg cccccggatc gctcggcctg acgtgtggtg
     3841 agacgctcgt cgtgcaggtc caccggcacg ccgaccctct ctgcgagccg ctccgcgaag
     3901 cggcgtgtct cctcggcctg cgggccctcc tcgccggaca gggtgagcgg aagccctacc
     3961 accacgcgct cggcgccctg cgcgcgcacg agcgcgacga ccctgtcgag cccccgtcgc
     4021 gtggccggtc gttcgaccgc gctcagcggc gtggcaagcg tgccggtggg atcggagacg
     4081 gccgccccgc agcgcgcctc cccgtaatcc agcgcaagca cgcggctcat ctaagcgttt
     4141 tttcgatcgc gaggcgtgct gccgcgatcg cctcgggcag cttctcggga tcgcgaccac
     4201 cagcctgcgc catgttgtcg cgtccgccgc ccccgccgcc ggcgacctcc gccgcggcgc
     4261 gcacgacgtc gccggccttg aggccgcgtt caaccgcgcc gggcgagaaa ttcgcgatca
     4321 ggtgcacgcg cccatcgatc gcagtgccga gcaccaccgc gctgtcgccg agcgtctgct
     4381 tgagctggtc ggagagctgg agcagcgtgt cgcggtccgg aacctcgacc acctgcgcga
     4441 gcacccgcac gccgccgatc tcctccgcct tctcggcaag cccctgcgcg agctggcgat
     4501 cgtcctgcct cggccgccgc tgcgcctcgc gcagctgctc ctgcagcttg tcgaccgccg
     4561 agaccacgtc acgctcgggc acgcgcagca tcgccgcgag ccggcgcatc tcctcggtgc
     4621 gctcgcggaa cacgtcgatg cctccggccc cggtgaccgc ctctacgcgg cgcacattgg
     4681 cggcgctcga ggtctcgtgc gttaggtgga agaggccgat ctcggccgag ctcgccacgt
     4741 gcgtgccgcc gcacagctcg cgcgagacgc cctcgatctc gaccatgcga acccaatcgc
     4801 cgtacttctc gccgaacagc gccatcgcgc cgaggcgctc ggcctcgtcg cgcgtcgtct
     4861 cgatcgcgcg caccgggtgg gtggcgacga tccactcgtt cacgaggtcg gtcaccgccg
     4921 cgagctggtc gggcgtgagg cgctcgccgt gggtgaagtc gaagcgcagc ttgtccggac
     4981 ccacgtacga gccc
//
LOCUS       DASVXS010000230         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1196732, whole genome shotgun sequence.
ACCESSION   DASVXS010000230 DASVXS010000000
VERSION     DASVXS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196732"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VF752_12950"
     CDS             complement(<1..655)
                     /locus_tag="VF752_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8856496.1"
                     /translation="MALHEQVQGFRRVADLYERNRPSFPDAAVDTIARVLDLGPGRHV
                     VDLGAGTGKLTRLLVPTGARITAVEPLAEMRARLEELVPDAETPAGTAEQLPVPDASA
                     DGIVVAQAWHWFDHARALAEARRVIRPGGALVVLWNDWDVSDPLVAELEAMRSRNVPA
                     GTPGHRAGDGMGPFESEAGFGPMQQATFPNEQLLNADGVVGRMLSVSYVASAPEDERE
                     "
     gene            691..1791
                     /gene="proB"
                     /locus_tag="VF752_12955"
     CDS             691..1791
                     /gene="proB"
                     /locus_tag="VF752_12955"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX8856497.1"
                     /translation="MSTLVLKLGSSIVADDRGELRADVLGALVDDVAALHGAGHSAVV
                     VTSGAIARGMRMMDLAMRPRSMEELQAASAVGQGKLYRSYDEMLRERGVTSAQVLLTF
                     FDMSARSHYLNARQTLRKLLDWRVVPVINENDTTTTDEISFGDNDFLAAQVAILLDAD
                     LLLMLTDTDALYDSDPRANPDARPVREVRDFSVLEELEIARTTSLLGSGGMRSKVVAA
                     EMATAAGIPAVIASGTEEGIVRRAFGGDDVGTRFLPLEGRVSSFKLWLKYAKPSRGRV
                     VVDVGAAHALKERGTSLLPVGVVGVEGEFEAGDAVDVSSAEGNGRAIGKGIVNYSADE
                     LRRVKGMKTIELRELLPHASEEAVHRDYFVLE"
     gene            complement(1773..2411)
                     /locus_tag="VF752_12960"
     CDS             complement(1773..2411)
                     /locus_tag="VF752_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX8856498.1"
                     /translation="MAHVLSVNVGRPQLISNGERTEKSSIRKQAVEGRVAVRGVNIEG
                     DDQADRAVHGGPDKAVYAYAREDTDWWESELGRELPNGMFGENLTTAGIDLSSLRIGD
                     RFRVGTAVLEVSEPRFPCWKLAARFKDPLMVRRFAKALRPGAYFRIAREGELGAGDEL
                     TLEERAAHHDVTIGFFARAFLEDRSKLELILAAPISDTWRHWITALAYSSTK"
     gene            2454..3725
                     /locus_tag="VF752_12965"
     CDS             2454..3725
                     /locus_tag="VF752_12965"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX8856499.1"
                     /translation="MATTARSVGEVCAGAKRAAHTLATLDAATKNAAIEAMADALEAR
                     TPEILAANERDMEAGREAGLSAALLDRLMLDEARVAGIAADARKIVALPDPVGETIEG
                     HRLENGLDVRKVRVPLGVVAVVYEARPNVTIDCSALCIKSGNAIVLRGSSSAAHSNAV
                     LAAIAAEAVASAGLPEGTVSLVQGGGREELAELATQDGLVDLVIPRGGEGLKNALKEH
                     ATVPVLYAASGNCHVYVDASADLGDAVAIAFNAKVQRPGVCNAAETLLVHADAAPTFL
                     PRALSELQGAGVELRVDGRTRSLAGELAPTLNEATEEDWETEFHALVLAVKVVDSVDE
                     AIEHINRYGSGHSEAIVTGSTKSAQAFTGGVDAACVYVNASTRFTDGAQFGMGAEIGN
                     STQKLHARGPIALRELCSYKYVIEGSGQVRP"
     gene            3731..4351
                     /gene="nadD"
                     /locus_tag="VF752_12970"
     CDS             3731..4351
                     /gene="nadD"
                     /locus_tag="VF752_12970"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX8856500.1"
                     /translation="MRVGIFGGAFNPPHIGHLVCAQEAHAHLALDTLVWVPMGQPPHR
                     EIEDDPGREARYTMCEYATSADERFSLSRFEIDREGPSYTVDTLRAVAESTPGAELFV
                     ILGADQAAALPTWHEPEEVLSLATVAAVARAERTREMVVDAVSSLAGGDRVVFFDMPR
                     IDVSSTLVRSRARAGQPIRYLVPDKVANYIGAQSLYGSSQPVGAER"
     gene            4348..4725
                     /gene="rsfS"
                     /locus_tag="VF752_12975"
     CDS             4348..4725
                     /gene="rsfS"
                     /locus_tag="VF752_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX8856501.1"
                     /translation="MTVEQPRERGGMSPEELAERIAAIASDRKAIDIHIIDLRELVGY
                     TDYFVICSGNTERQTKAVHDAIYRELKDVEGLLPRRVEGEREARWILMDYLDVVVHIF
                     TPEAREFYRLESLWGDAPVRAVQ"
     gene            5002..>5414
                     /locus_tag="VF752_12980"
     CDS             5002..>5414
                     /locus_tag="VF752_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856502.1"
                     /translation="MFIRVNADTCGSPAFHPTVALELAPSEAVRVRADGTFSDTSRVT
                     GQTLVEGKRLDFQAVLKGRVGRARAAGTIRMFGPVRDAAGNIVDTCDSGVVRWALARG
                     PGFYGGATDQFGAVSIQVDRGGPEVRVFFVDFEVT"
BASE COUNT          799 a         1773 c         2011 g          831 t
ORIGIN      
        1 gctcccgttc gtcttccggg gcggaggcga catagctcac cgacagcatc cggccgacga
       61 cgccgtcggc gttgagtagc tgctcattcg ggaaggtcgc ctgctgcatg ggcccgaagc
      121 cggcctctga ctcgaacggg cccatgccgt ctccggcccg gtggcccggc gttccggccg
      181 gaacgtttcg cgagcgcatc gcctcgagct ccgccaccag cggatcggac acgtcccagt
      241 cgttccagag cacgacgagc gcgccgccgg ggcggatcac gcgccgtgcc tccgcgagcg
      301 cacgggcgtg gtcgaaccag tgccatgcct gcgcgaccac gatcccgtcg gcgctcgcgt
      361 ctggcaccgg cagctgctcg gcggtccccg cgggcgtctc tgcgtcgggc acgagctcct
      421 cgagccgggc gcgcatctcg gccagaggct caaccgcagt gatgcgcgcg ccggtgggga
      481 cgagcagccg cgtgagcttg ccggtgccgg ctccgaggtc tacgacgtgg cggccgggac
      541 cgaggtcgag aacgcgcgcg atcgtgtcga ccgccgcgtc cgggaagctc ggcctattgc
      601 gctcgtacag atccgccacg cgccggaagc cctgaacctg ctcgtggagc gccacgcgcg
      661 gcaggctact cccctcataa ccttgtcccg atgtccactc tggtcctgaa gctcggttcg
      721 agcatcgtcg ccgacgaccg cggcgagctc cgcgcggacg tgctcggcgc gctcgtggac
      781 gatgttgctg cgctgcatgg ggcggggcac tcggcggtcg tcgtcacctc gggggcgatc
      841 gcgcgcggca tgcgcatgat ggacctcgcg atgaggccgc gctcgatgga ggagctgcag
      901 gcggcgtcgg cggtggggca gggcaagctg taccgctcct acgacgagat gctgcgcgaa
      961 cgcggcgtga ccagcgcgca ggtactgctc acgttcttcg acatgtccgc gcgcagccac
     1021 tacctgaacg cgagacagac gctccgcaag ctgctcgact ggcgggtggt gccggtcatc
     1081 aacgagaacg acacgacgac cacggacgag atcagcttcg gcgacaacga cttcctcgcc
     1141 gcccaggtgg cgatcctgct ggacgccgac ctgctgctga tgctcaccga cacggacgcg
     1201 ctctacgact cggatccgcg ggcgaacccg gacgcgcggc ccgtgcgcga ggtgcgcgac
     1261 ttctccgtgc tggaggagct cgagatcgcg cgcaccacat cgctgctcgg ctcgggcggc
     1321 atgcgctcga aggtggtggc ggccgagatg gcgacggcgg cggggatccc cgcggtgatc
     1381 gcgagcggga cagaggaggg catcgtacgg cgcgcgttcg gtggcgacga tgtgggcacg
     1441 cgcttcctgc cgctggaggg tcgcgtgtcg agcttcaagc tctggctcaa atacgccaag
     1501 ccgagccgcg ggcgcgtggt ggtcgacgtc ggcgcggcgc acgcgctcaa ggagcggggc
     1561 acgagcctgc tgcctgttgg cgtcgtggga gttgaggggg agttcgaggc cggtgacgcc
     1621 gtcgacgtgt cctccgccga ggggaacggg cgggcgatcg gcaagggcat cgtcaactac
     1681 tccgccgacg agctgcggcg ggtgaagggg atgaagacga tcgagctgcg cgagctgctg
     1741 ccgcacgcct ccgaggaggc cgtgcaccgg gattacttcg tgctcgagta ggccaatgcc
     1801 gtgatccagt gccgccaggt gtccgagatg ggcgcggcca agatcaactc gagtttcgag
     1861 cggtcttcaa ggaaggcgcg tgcgaagaag ccgatcgtca cgtcgtggtg cgcggctcgt
     1921 tcctccaggg tcagctcgtc gccggcgccg agttcgccct ccctcgcgat gcggaagtag
     1981 gccccgggcc ggagcgcctt cgcgaaacgc ctgaccatga gcggatcctt aaagcgcgcg
     2041 gcgagcttcc agcacgggaa acgtggctcg ctcacctcga ggacagccgt tccgactcgg
     2101 aagcggtcgc cgatccgcaa gctcgagagg tcgataccgg cggtcgtgag gttctcgccg
     2161 aacatgccgt tcgggagctc gcggccgagc tctgactccc accagtcggt gtcctcgcgc
     2221 gcgtacgcgt agacggcctt gtcgggcccg ccgtggaccg cgcggtcggc ctggtcgtcg
     2281 ccctcgatgt tcacgccgcg gacggccacg cgaccctcga ccgcctgctt gcggatcgag
     2341 ctcttctcgg tgcgctcgcc gttggagatg agctgagggc ggccaacgtt caccgacagc
     2401 acatgagcca tctcttcaca ctagcccgcg gcgcctccca gtacccttgt gcgatggcca
     2461 ccacagcacg atccgtaggc gaggtctgcg ccggcgcgaa gcgcgccgca cacacgctgg
     2521 ccacgctcga cgccgcgacg aagaacgccg cgatcgaggc gatggccgac gcgctcgagg
     2581 cgcgcacacc ggagatcctc gccgcgaacg agcgcgacat ggaagcgggc cgcgaggcgg
     2641 gcctctcagc ggcgctgctc gatcggctga tgctcgacga ggcgcgcgtt gcggggatag
     2701 ccgcggacgc gcgaaagatc gtcgcgctgc cggatccggt gggcgagacg atcgaggggc
     2761 accggctcga gaacgggctc gacgtgcgca aggtgcgcgt gccgctcggg gtggtggcgg
     2821 tggtgtacga ggcgcgtccg aacgtgacga tcgactgctc ggcgctctgc atcaagtcgg
     2881 gcaacgcgat cgtgctgcgc ggctcgtcgt cggcggcgca ctcgaacgcg gtgctggcgg
     2941 cgatcgcggc ggaggcggtc gcgtcggcgg ggctgcccga aggcaccgtg tcgctcgtgc
     3001 agggcggcgg tcgcgaggag cttgccgagc tcgccacgca ggacgggctc gtcgacctcg
     3061 tcatcccgcg cgggggcgag gggctgaaga acgcgctgaa ggagcacgcg accgtacccg
     3121 tgctctacgc ggcgtccggc aactgccatg tgtatgtgga tgcgtcggcg gatctcggtg
     3181 acgcggtggc gatcgcgttc aacgcgaagg tgcagcggcc cggcgtctgc aacgcggccg
     3241 agacgctgct cgtgcacgcc gacgccgcgc ccaccttcct cccgcgtgca ctttccgagc
     3301 tgcagggcgc tggcgtggag ctgcgcgtgg acgggcggac gcgctcgctc gccggcgagc
     3361 tggcgccgac gctcaacgag gcgaccgagg aggactggga gaccgagttc catgcgcttg
     3421 tgctcgcggt caaggtggtc gactccgtag acgaggcgat cgagcacatc aaccgctacg
     3481 gaagcgggca ctctgaggcc atagtgaccg gctcgacgaa gtccgcgcag gcgttcaccg
     3541 gtggtgtgga cgctgcctgc gtctacgtca acgcgtcgac gcgcttcacg gacggggcgc
     3601 agttcgggat gggggcggag atcggcaact cgacccagaa actgcacgcg cgtgggccga
     3661 tcgcgcttcg cgagctctgc tcctacaagt acgtgatcga gggctccggc caggtgaggc
     3721 cgtagcgggt ttgcgggtag gcatcttcgg cggggcgttc aacccgccgc acatcggcca
     3781 cctggtgtgt gcgcaggagg cgcacgccca cctcgcgctc gacacgctgg tgtgggtgcc
     3841 gatgggacag ccgccgcacc gggagatcga ggacgatccc ggccgcgagg cgcgctacac
     3901 gatgtgcgag tacgcgacgt cggcggacga gcgcttctcg ctgtcgcggt tcgagatcga
     3961 tcgcgagggg ccgtcgtaca cggtcgacac gctgcgggcg gttgccgaaa gcacgcccgg
     4021 agccgagctg ttcgtcatcc tgggcgccga ccaggcggcg gcgctgccta cgtggcacga
     4081 gcccgaggag gtgctctcgc tcgccacggt ggcggcggtc gcgcgagcgg agcgcacgcg
     4141 ggagatggtg gtcgatgccg tgtcgtcgct ggcgggtggc gatcgggtgg tgttcttcga
     4201 catgccgcgt atcgacgtgt cgtccacgct cgtgcggtcg cgggcgcggg cgggccagcc
     4261 gattcgctac ctcgtgcccg acaaggtcgc gaactacatc ggggcgcaga gcctgtacgg
     4321 gtcgtcgcag ccggtggggg cggagcggtg acggtcgagc agccgcgcga gcggggcggg
     4381 atgtcgccgg aggagctggc ggagcggatc gcggcgatcg cgtccgaccg caaggcgatc
     4441 gacattcaca tcatcgacct gcgcgagctc gtcgggtaca ccgactactt cgtgatctgc
     4501 agcggcaaca ccgagcggca gacgaaggcg gtccatgacg cgatctaccg cgagctgaag
     4561 gacgtcgagg gactgctgcc gcggcgcgtg gagggggagc gcgaggcgcg ctggatcctc
     4621 atggactacc tcgacgtggt ggtccacatc ttcacgcctg aggcgcggga gttctaccgg
     4681 ctcgagagcc tctggggcga cgcgcctgtg cgcgcggtgc agtaggccgt tcagcggcgc
     4741 agcgtcgctt gactcggttc tctgccgggt ctagcgtcgg ccgcgttaac cacttccacc
     4801 ggtgagggca caccgaccgt ggagggcaga gactcgacac gccggcttca tctcattccg
     4861 ctcggcattc tgctcgcgct ggcgctgccg tcgccggcac tcgcttcggg tgggcccgcg
     4921 ggcggcgtgt atgccggcgc gagctcccag cgtcatccgg tcagtctcca actcacgcgc
     4981 gacgccagca ggatccggac cttgttcatc cgcgtcaatg cggatacctg cggctctcct
     5041 gctttccacc cgaccgtggc gctcgagctc gcaccgagcg aagcggtgcg tgttcgggcg
     5101 gacggaacgt tcagcgacac gagccgggtc acggggcaga cgctggtcga gggcaagagg
     5161 ctggacttcc aggcggtgct gaagggaagg gtcgggaggg cccgagcggc tgggacgatc
     5221 cgtatgttcg gtccggtcag ggacgcggcg ggcaacatcg tcgacacctg cgactccggt
     5281 gtcgtgcgat gggcgctcgc gcgcggtccc ggtttctatg gtggcgcgac ggaccagttc
     5341 ggtgccgtga gcatccaggt ggatcgaggc ggcccggagg tccgcgtgtt cttcgtcgat
     5401 ttcgaggtca cttg
//
LOCUS       DASVXS010000231        13883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1235315, whole genome shotgun sequence.
ACCESSION   DASVXS010000231 DASVXS010000000
VERSION     DASVXS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13883
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235315"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VF752_12985"
     CDS             <1..254
                     /locus_tag="VF752_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX8856503.1"
                     /translation="NVRTRVGLEAPELPTVTDLYPGANYLEREIYDMFGVVFAGHPDM
                     RRILMPEDYTGYPQRRDFPMGGEDVIYTFNEDTLPRWYD"
     gene            247..1569
                     /gene="nuoD"
                     /locus_tag="VF752_12990"
     CDS             247..1569
                     /gene="nuoD"
                     /locus_tag="VF752_12990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX8856504.1"
                     /translation="MTDQVGNGDTGPGMQSTGVDPLAVPYREREQSITIERLDHPDQP
                     IAGTELEQETELFTINLGPHHPATHGVLRLLLTLEGEVVRSCVPLIGYVHTGIEKSCE
                     DQQYWKVIPFVERMDYLSYYFNAMAFCMAVENLLDEEVPPRAQYLRVIHLELNRIASH
                     LFWIATSALDLGAITMLWWGIRDRDKILDLFEMSSGQRMHPRYFQVGGVIEDIPPGWE
                     DRCRDFIRYMPSRIDQYEALLDRNEIFLQRLKNVGVVDKDTLLGLGVTGPLLRAAGIP
                     WDLRKAEPCSSYEHFDFKIPVGTVGDHYDRYRVRMAEMRESLKIIEQALDGLPEGKHI
                     TDNRKVALPPRHELATSMEALIHHFKLVTEGYRVPPSETYQALESPRGELGCFVLADG
                     SAKPARVHVRDPSFVNLQMLPHMAQDGLIADLIASIAMLDPILGGIDR"
     gene            1566..2261
                     /locus_tag="VF752_12995"
     CDS             1566..2261
                     /locus_tag="VF752_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX8856505.1"
                     /translation="MTDSPRGNGRSLRESDPLLADRIVGTPEQQAAARPAVAAGWDEP
                     ADIEKAPATIPDLSEVEVPPALKAEIEAHMAKYPNVRSAALPSLEAAQRVHGWCSPEA
                     LRQVAAVMRVTPAYLSMLATFYDMLNTEPVGRHYVYVCTSVSCHLRNAQALAEAMKAA
                     KQEGDDVNVREFECLGACDMAPMANVDGRFIGPIDPSEAPEVLAALREGRTPLPGRGL
                     GDEGFRLPWEGES"
     gene            2261..3583
                     /gene="nuoF"
                     /locus_tag="VF752_13000"
     CDS             2261..3583
                     /gene="nuoF"
                     /locus_tag="VF752_13000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX8856506.1"
                     /translation="MAVQEVRVLLDNIDEPGLNHIDTYERLGGYQAMRKALTEMEPDE
                     VLAALENSGLRGRGGAGFSMGKKASFLPKGSMDKYLCCNADESEPGTFKDRLLMQKNP
                     HRLIEGIIIAAIAAGANKAFIYIRGEYVLQADVLDAAVREAYDKGYLGQNILGSRNDV
                     DLVVHRGAGAYICGEETALLDSLEGKRGNPRLKPPFPANQGLYNGPTLINNVETLNNV
                     PLVIAKGVDWFKQFGTEQSTGTKVVSVSGNVQRPGNYEIALGIPARDLVYGLAGGPPP
                     GRKIKCWFPGGSSAPVLLEEDLDRPYSFEAMAEAGSMLGSGAIIVVDDSVPIVSVALR
                     LAQFYRHESCGKCVPCREGTNWTVKMLERIDTGDATPMDLEIMAQVQTNIIGNCLCVL
                     GDSMAMPIGSMIEKFRDEFVEHIERAREAKDRLSVELGTYAEPADAVA"
     gene            3589..6078
                     /gene="nuoG"
                     /locus_tag="VF752_13005"
     CDS             3589..6078
                     /gene="nuoG"
                     /locus_tag="VF752_13005"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX8856507.1"
                     /translation="MPKPEPKLITFEIDGREVQAPEGQMLVDAAKMGDVEIPYFCYEA
                     KLGQPVGACRMCLVEVEGIPKLQTSCSTPVKDGMVVNTKSDRVKQAQNAIVEFLLVNH
                     PLDCPVCDKGGECPLQDISYGWGPGRSRFIEPKRHFEKPVALSPLVAIDRERCILCYR
                     CVRFSQELSEDYQLVFLERADHTFVGTHDGHPYVAPFSGNIIELCPVGALTSQAYRFR
                     ARPWDIEDAGSICTLCPSQCNVKYTTRDDNKVVRVLARDNEDVDDGWLCDKGRFGYQA
                     IHVPERVTRPMVRDGGELRPVSWERALSEAAGALARAGDRTAALVGGEATNEDGFLTQ
                     HLLRGVLDSPHVDSRTSGVLDPQQQAVLAQPDLTAKVSDIEWAGTVLVLDTELVDESP
                     ILDLRVRKGVRRNGLNVIVATSRPSTLDTNARALLRFAPGAGEALLAALAAAVSGQGD
                     LEDLSGRAGTSAEILRGAAELLQEPGPVVVLWGERLSQGERGRQAVARLIDLARQLNL
                     SGTEGAGLLEVPRGTNARGLREVGCVPALGPGLDTATRAGKSAGEIAASVPGEVSALL
                     LVGADPLTTHPDRGMWERALDRATSVIAFADFVTEGLDEHADVVFPAESEAEKDGTMT
                     HPDGRIQRVRQAIARPGEVRPQWSVLAELCERLGNPTGLLSGAMVSERVFESVPFYEG
                     ITLAEIGGKGVRWQERDGAARLPRVELPVGELEIPPRATAEGLRLGTGPSIWSNRVVE
                     HSPLLRFLLPEQVVELSPEDGERLGLRTGDQVSVSVDGTSVSGRAFLRSDMPPGNVFL
                     IEGTRTDNATALLNGKPRSIELLRAEETSPA"
     gene            6075..7079
                     /gene="nuoH"
                     /locus_tag="VF752_13010"
     CDS             6075..7079
                     /gene="nuoH"
                     /locus_tag="VF752_13010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX8856508.1"
                     /translation="MSVLAVVDYSEAVWIQILKAIVIFAVILQIVPIVLLIERKLLGR
                     FQSRLGPNRVGKWGLLQPLADVLKLLSKEQFATETAVPWMFAIAPVISIFTAVATLAI
                     IPFGDVGTWGGTIGLYGIDVSIGILYAFAFGALAFYGLMLGGWASGSKYSFLGAMRSA
                     AQLISYEIALGLSLLGVAIQAGSLSLVEIVHAQDNYWFITTQFVGFLIFTVAGFAETN
                     RAPFDLPEADAELVGGYNTEFGGMRFGSFFMAEYINMIVISGIATAMFLGGWRGPGPE
                     ALGPLWVLIKIFLFLVFFIWVRATLPRLRYDQLMSFGWKVLLPLATVNVIVTAIVVAT
                     "
     gene            7084..7620
                     /gene="nuoI"
                     /locus_tag="VF752_13015"
     CDS             7084..7620
                     /gene="nuoI"
                     /locus_tag="VF752_13015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX8856509.1"
                     /translation="MTPYNPSPDIIEVQRGPNPGGLGGAYRTFGATLRGLKTTLGRVV
                     EGPVTLEYPEQKTPVYPRFRGKHKLHRFEDTGLEKCVGCSLCAAACPAECIRVVAAEN
                     TPDHQVSAGERYAAVYEINMLRCIFCGYCELACPFDAITMGNDYELSDYTRSDLIFTK
                     EMLLADHPERTPLRREGE"
     gene            7620..8234
                     /locus_tag="VF752_13020"
     CDS             7620..8234
                     /locus_tag="VF752_13020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX8856510.1"
                     /translation="MVFKDIVFFIAAIGALTGAIGVVMLRNPFFSVLALVGHLFSLAV
                     LFLLLSSQFLAAAQLVVYAGAVMVLYVFVVAYIGGADEPLGPQIPGVGTLGPLVAAAL
                     CGELFFAFIGTGLEAVDSRGAKIGSGFGSPGQIGSLLLNNFLIPFEAASFILLVAAVG
                     AVVLARRRRGLDEDEGGAPVDVITHERLPETHPQRGGVTSEVGS"
     gene            8231..8530
                     /gene="nuoK"
                     /locus_tag="VF752_13025"
     CDS             8231..8530
                     /gene="nuoK"
                     /locus_tag="VF752_13025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX8856511.1"
                     /translation="MNLVWYLCLSAAIFSVGAAGVLIRRNPLVILLCLELMLNAGNLA
                     LITFARMHGNGDGQIFALVVMVVAACEVVVGLGVIVAMYRNRIAEDVDEMSELRG"
     gene            8527..10536
                     /gene="nuoL"
                     /locus_tag="VF752_13030"
     CDS             8527..10536
                     /gene="nuoL"
                     /locus_tag="VF752_13030"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX8856512.1"
                     /translation="MSATTWGWLVLAFPLAGTILLPLGFKFWPGRLPGWIGSLTILLS
                     FGASLGALFNIMGEHPEQRSHYDTLWTYASTAKFNAGLTILVDPLSVFMCLVVTGVSF
                     LIHVYSISYMGGDRGYTRFFTYLNYFVFSMLLLVLGGNIVILIIGWAFVGAASYLLIS
                     FWYRRRTATAAGMKAFVINVIGDVGLVIAAFLLWNKTGALDWAGIFSGAKDAFSSNDG
                     TLIAACLFLTMGAFAKSAQVPLHTWLPDAMEGPTPVSALIHAATMVTAGVYLVARTHP
                     LYELAPTAADITAALATFTLLYAATCALAITDFKRIIAYSTISQIGYMMLGVATFGYQ
                     FGLFLLMAHAFFKALLFMGAGSVISAMGGEQNIDRMGGFRRAMPFTYITFTIGALALA
                     GLPIFAGWLAKDGILGWTFHRGGWYIALGLVGYLTALLTAFYSFRMVFRVFFGEPVRE
                     AQLLEGGELVHGEHRNPLSGEEEDTDVGFPGPEHHIAEREATMKVAMSLLAVLSLAGG
                     IVLIPGVTEWLQAFLEPTFSDSGFVGSHVPTGSEWLGLGVGAVAAAAGIAAAWFAYRA
                     RPGMTLELRDRFGRVHAFFFNRWYFDELFDVIFVRPTLAVARFFQRVIEPGVVQGVLV
                     GGATGVVRLSTNFARSIQSGYLRAYAGLLVTGLFGLVLYFLIQSS"
     gene            10540..12066
                     /locus_tag="VF752_13035"
     CDS             10540..12066
                     /locus_tag="VF752_13035"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX8856513.1"
                     /translation="MHLTIPLFIPLAAGIVGLLLPRGLARWASLAGTLAVLGYSVAMI
                     VDFDSATRGLQWVTNETWIDTLGIHYKLGVDGLNLFLILATAFLWVIATLYAAVREWD
                     RPRLFYFNLGLAETAVLGAFLAQDLALFVVFFDLMLVPFYFLIGAWGRGNRVYATTKF
                     VIYTLSGSLLMLAGAVALGVLSTPEGGQISFQLSDLTVHPVGHGTQVWIFLLFAAAFL
                     IKAPAFPLHGWVPETYRATPIPVLILLSGILSKVGMYAFLRIVIPIMPGASATLQELM
                     LVIAVVSILYGSVLAFSQDDARLVVAYSSIAQLGFILLGIFALDPKSAQGSVIQMLNH
                     AVVVAGLFVIIGVIAARARESESLERLGGMALRAPVLAGLFLIVALATLAMPGSPNFV
                     GEILILFGTFDEKFVYGLVASVGVVLAAVYMIRLYQRTMHNRAGPDVASSDLSYRLDL
                     PALAPVALVLIALGLYPQFVLHRTEASTTPKIVRAAIKAGSDPGPQHAEATTVKGATP
                     "
     gene            12063..13616
                     /locus_tag="VF752_13040"
     CDS             12063..13616
                     /locus_tag="VF752_13040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX8856514.1"
                     /translation="MILTLAAKVTPPVIDYKGFSPLIALVGGTVLVLLVALFRGRAVQ
                     RGLVPLLTAGTLLTAMGLSVWVWHAGDSRPIVEGAIERDALAMGMSMIFYVAGLATVL
                     LSHRSVAVREAGAGEYYMLLLSSVTGMVLLAQAANLVTLFVALELLSIPLYVLCASHV
                     RRRTSLEAGLKYLVIGSLGSATLLYGLAMIYGATGTTSFAGIDNALGDTVSIADPLFL
                     TGVGLALTGFAFKASVAPFHQWTPDVYQGAPTPITAFMAVATKAAAFAMFLRVLDQAL
                     PSAQTYWAPALATLAVITIIVGNVGAIAQSSLKRMLAWSGVAQAGYIAAGVVVGTQKG
                     LEATAFYLFVYLLMNVGAFAVIVARERVSEFGDDIRSLEGLGRAAPWLAWPMTICMLA
                     LAGFPATGGFIGKFYLIDAAVSGDYAWLGIVIVAGSVISLVYYLRVIAVMFMGPFEIT
                     LPAMPGRPARRVKPVGGWSPEADAWRQPEVVIVALLCAAATIFFGVWPNPLFDVVRDM
                     GTSIQSLIG"
     gene            13624..>13883
                     /locus_tag="VF752_13045"
     CDS             13624..>13883
                     /locus_tag="VF752_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856515.1"
                     /translation="MSLRVRWVIAALVGAALIGGLIWAYSGDESFTRATAEDQLQQFH
                     DERRPNTPAENVRCETAGSAWRCRFREDGKECAGAVSGKPTSP"
BASE COUNT         2002 a         4800 c         4685 g         2396 t
ORIGIN      
        1 tgaacgtgcg cacgcgcgtg ggactcgagg cgcccgagct gccgaccgtc accgacctct
       61 atcccggcgc caactacctg gagcgcgaga tctacgacat gttcggcgtg gtcttcgcgg
      121 ggcaccccga catgcgccgc atcctgatgc ccgaggacta cacgggctac ccgcagcgcc
      181 gcgacttccc catgggcggc gaggacgtga tctacacgtt caacgaggac actcttcccc
      241 gctggtatga ctgaccaggt cggcaacgga gacaccgggc cgggcatgca gtcgaccggc
      301 gtcgatccgc tcgcggtgcc ctaccgcgag cgcgagcagt cgatcacgat cgagcgactc
      361 gatcacccgg accagccgat cgcgggcacc gagctggagc aggagaccga gctcttcacg
      421 atcaacctcg ggccccacca ccccgcgacg cacggcgtgc tgcggctgct gctcacgctc
      481 gagggagagg tcgtgcgctc gtgcgtgccg ctgatcggct acgtgcacac ggggatcgag
      541 aagagctgcg aggaccagca gtactggaag gtgatcccgt tcgtcgagcg gatggactac
      601 ctgtcctact acttcaacgc gatggcgttc tgcatggccg tcgagaacct gctcgacgag
      661 gaggtcccgc cgcgcgctca gtacctgcgc gtgatccacc tcgagctgaa ccgcatcgcg
      721 agccacctgt tctggatcgc cacctcggcg ctcgacctcg gcgccatcac catgctgtgg
      781 tggggcatcc gcgaccgcga caagatcctg gacctgttcg agatgtcgtc gggtcagcgc
      841 atgcatccgc gctacttcca ggtgggcgga gtcatcgagg acatcccgcc gggctgggaa
      901 gaccgctgcc gcgacttcat ccgctacatg ccgtcgcgca tcgaccagta cgaggcgctg
      961 ctcgaccgca acgagatctt ccttcagcgg ctgaagaacg tcggggtggt cgacaaggac
     1021 acgctgctcg gactgggcgt gacaggcccg ctcctgcgcg cggcgggcat cccgtgggac
     1081 ctgcgcaagg cggagccctg ctcgtcctac gagcacttcg acttcaagat cccggtgggc
     1141 acggtgggcg accactacga ccgctacagg gtgcgcatgg ccgagatgcg tgagtcgctg
     1201 aagatcatcg agcaggcgct cgatggtctt cccgagggca agcacatcac cgacaaccgc
     1261 aaggtcgcgc taccgccgcg ccacgagctc gcaacctcga tggaggcgct catccaccac
     1321 ttcaagctgg tgaccgaggg ctaccgcgtc ccgccgtccg agacctacca ggcactcgag
     1381 tcgccgcgcg gcgagctcgg atgcttcgtg ctcgccgacg gctcggccaa gcccgcgcgc
     1441 gtgcacgtcc gcgacccctc cttcgtgaac ctgcagatgc ttccgcacat ggcgcaggac
     1501 ggcctgatcg cggacttgat tgcatccatc gccatgctgg atcccatcct cggaggcatc
     1561 gaccgatgac cgactcgccg cggggcaacg ggcgctcgct gcgcgagtcc gatccactgc
     1621 tggccgaccg gatcgtgggc acgccggagc agcaggcggc cgccaggccg gcggtggccg
     1681 ccggctggga cgagcccgcc gacatcgaaa aggcgcccgc cacgatcccg gatctgtccg
     1741 aagtggaagt gccgccggcg ctcaaggccg agatcgaggc gcacatggcg aagtacccga
     1801 acgtccgctc cgccgcgctg ccttccctcg aggcagcgca gcgggtgcac ggctggtgct
     1861 cgccggaggc gctgcgacag gtggccgcgg tcatgcgcgt caccccggcg tacctgtcga
     1921 tgctcgcgac cttttacgac atgctcaaca cggagcccgt cggccgccac tacgtatacg
     1981 tctgcacgag cgtgagctgc catctccgca acgcgcaggc cctcgctgag gcgatgaagg
     2041 cagcgaagca ggagggcgac gacgtgaacg tgcgcgagtt cgagtgcctc ggagcgtgcg
     2101 acatggcgcc gatggcgaac gtcgacgggc gcttcatagg cccgatcgac ccgagcgagg
     2161 cacccgaggt gctcgccgcg cttcgagagg gccgcacccc gctgccgggc cgcgggctcg
     2221 gcgacgaggg cttcaggctg ccgtgggagg gcgagagctg atggccgtcc aggaagtgcg
     2281 cgtgctcctc gacaacatcg acgagcccgg cctcaaccac atcgacacct acgagcgcct
     2341 cggcggctat caggcgatgc gcaaggcgct cactgagatg gaacccgacg aggtgcttgc
     2401 cgcgctcgag aactcagggc tgcgcggtcg cggcggcgcc ggcttctcga tgggcaagaa
     2461 ggcgagcttc ctgccgaagg gctccatgga caagtacctc tgctgcaacg cggacgagtc
     2521 cgagccgggc acgttcaagg accggctgct gatgcagaag aacccgcacc ggctgatcga
     2581 gggcatcatc atcgccgcga tcgccgccgg cgcgaacaag gccttcatct acatccgcgg
     2641 cgagtacgtg ctgcaggccg acgttctcga cgcggccgtg cgcgaggcct acgacaaggg
     2701 ctacctcggc cagaacatcc tcggctcccg caacgacgtc gacctcgtgg tgcaccgcgg
     2761 cgccggcgcc tacatctgcg gcgaggagac ggcgctgctc gattcgctcg aaggcaagcg
     2821 cggcaacccg cgcctgaagc cgccgttccc agcgaaccag ggcctctaca acggcccgac
     2881 gttgatcaac aacgtggaga cgctcaacaa cgtgccgctc gtgatcgcga agggcgtcga
     2941 ctggttcaag cagttcggca ccgagcagtc caccggaacc aaggtcgtct cagtgtcggg
     3001 caacgtacag cggcccggca actacgagat cgcgctcggc atcccggcgc gcgacctcgt
     3061 gtacggtctg gcgggcggcc cgcccccggg ccgcaagatc aagtgctggt tcccgggcgg
     3121 ctcctccgcg ccggtgctgc tcgaggagga cctggaccgc ccttactcgt tcgaggcgat
     3181 ggcggaggcg ggctccatgc tcggctccgg cgcgatcatc gtggtcgacg actccgtccc
     3241 gatcgtgtcg gtggctctgc ggctcgcgca gttctaccgc cacgagtcct gtggcaaatg
     3301 cgtcccctgc cgcgagggca ccaactggac ggtgaagatg ctcgagcgca tcgacacggg
     3361 cgatgcgacg ccgatggacc tcgagatcat ggcccaggtc cagacgaaca tcatcggcaa
     3421 ctgcctctgc gtgctcggcg actcgatggc gatgccgatc ggctcgatga tcgagaagtt
     3481 ccgcgacgag ttcgttgagc acatcgagcg cgcgcgcgag gcgaaggacc ggctcagcgt
     3541 cgagctcggg acgtacgcgg agccggcgga cgccgtcgca tagacacgat gccgaagccc
     3601 gagcccaagc tgatcacgtt cgagatcgac ggccgcgagg tgcaggcacc cgagggccag
     3661 atgctcgtgg acgccgccaa gatgggcgac gtcgagatcc cctacttctg ctacgaggcg
     3721 aagctgggcc agccggtcgg cgcctgccgc atgtgcctcg tcgaggtgga gggcatcccc
     3781 aagctccaga cctcgtgctc gacgccggtc aaggacggca tggtggtgaa caccaagtcc
     3841 gaccgcgtga agcaggcgca gaacgcgatc gtcgagttcc tgctcgtgaa ccacccgctg
     3901 gactgcccgg tctgcgacaa gggcggcgag tgcccgctgc aggacatctc ctacggctgg
     3961 ggcccggggc gctcgcgctt catcgagccg aagcgccact tcgagaagcc ggtggcgctg
     4021 tcgcctctcg tcgcgatcga ccgtgagcgc tgcatcctct gctaccgctg cgtgcgcttc
     4081 tcgcaggagc tgtccgagga ctaccagctg gtgttcctcg agcgcgcgga ccacacgttc
     4141 gtcggcacac acgacggcca cccgtacgtg gcgccgttca gcgggaacat catcgagctc
     4201 tgcccggtgg gcgcgctcac ctctcaggcg taccgcttcc gcgcgcgccc gtgggacatc
     4261 gaggacgctg gctcgatctg cacgctgtgc ccgagccaat gcaacgtgaa gtacacgacg
     4321 cgcgacgaca acaaagtcgt gcgcgtgctc gcgcgcgaca acgaggacgt cgacgacggc
     4381 tggctctgcg acaaaggccg cttcgggtac caggcgatcc acgtgcccga gcgcgtcacg
     4441 cgcccgatgg tgcgcgacgg cggcgaactg cgcccggtct cgtgggagcg cgcgctgtca
     4501 gaggccgctg gcgcgctcgc gcgcgcggga gaccgcaccg ccgcgctcgt cggcggcgag
     4561 gccacgaacg aggacggctt cctcacccaa cacctgctgc gcggggtgct cgactcgccg
     4621 catgtcgact cgcgcacgag cggcgtgctc gacccgcagc agcaggccgt gctcgcgcag
     4681 cccgacctga ccgcgaaggt ctcggacatc gagtgggccg gcaccgtgct ggtgctcgac
     4741 accgagctcg tcgacgagtc gccgatcctt gacctgcgcg tgcgcaaggg cgtgcgccgc
     4801 aacggtctga acgtgatcgt ggccacgagc cggccgagca cgctcgatac caacgcccgt
     4861 gcgcttctgc gcttcgcccc gggcgccggc gaagcgcttc ttgcagcact tgcagcggct
     4921 gtgtccggcc agggagactt ggaggacctg agcggccgcg ccggcacaag cgccgagatt
     4981 cttcgcggcg ccgccgagct tctgcaggag ccgggacccg tcgtggtcct gtggggcgaa
     5041 cggctctcgc agggcgagcg cggccgccag gcggtcgccc gcctcatcga tttggctcgg
     5101 cagctgaacc tttctggcac cgagggcgcc ggcctactcg aggtgccgcg tggcacgaac
     5161 gcgcgcggcc tgcgcgaggt gggctgcgtg cccgcgctcg gaccggggct tgacacagcc
     5221 acccgcgcgg gcaagagcgc cggtgagatc gctgcgagcg tgccgggcga ggtgtctgcg
     5281 ctgctgctcg ttggcgccga cccactcacc acacaccccg accggggcat gtgggagcgc
     5341 gcgctcgacc gcgccaccag cgtgatcgca ttcgccgact tcgtgaccga gggtctcgac
     5401 gagcacgccg acgtggtctt ccccgccgag tccgaggcgg agaaggacgg caccatgact
     5461 caccccgacg ggcggatcca gcgtgtgcgc caggcgatcg cgcggccggg cgaggtgcgc
     5521 ccgcagtgga gcgtgctcgc cgagctgtgc gagcgtctcg gcaatccgac cggcctcctg
     5581 tccggcgcga tggtctccga gcgggtgttc gagagcgtgc cgttctacga gggcatcacg
     5641 ctcgcggaga tcggcggcaa gggcgtgcgc tggcaggagc gcgacggcgc cgcgaggctg
     5701 ccgcgcgtcg agctgcccgt cggtgagctc gagattccgc cgcgcgctac cgcggaaggc
     5761 ctgcggctcg gcaccggccc atcgatctgg tcgaaccgcg tggtcgagca ctcgccgctc
     5821 ctgcgcttcc tgctgcccga gcaggtggtc gagctgtcgc ccgaggatgg agagcgactc
     5881 ggcctgcgga cgggcgatca ggtgagcgtc tcggtggacg gcacctcggt gagcggccgc
     5941 gcattcctgc gctccgacat gccgcctggc aacgtctttc tgatcgaggg cacgcgcacc
     6001 gacaacgcca ccgcgctcct caacggcaag ccgcgcagca tcgagctgtt gcgtgcggag
     6061 gagacgtcgc ccgcgtgagc gtcctcgcag tcgtcgacta cagcgaggcg gtctggatcc
     6121 agatcctcaa ggcgatcgtg atcttcgccg tgatcctcca gatcgtcccg atcgttctcc
     6181 tgatcgagcg caagctgctc gggcgcttcc agtcgcgcct cggaccgaac cgcgtgggca
     6241 agtggggtct gctgcagccg ctcgccgacg tgctgaagct gctctcgaag gagcagttcg
     6301 ccaccgagac cgcggtgccg tggatgttcg cgatcgcgcc ggtgatctcg atcttcaccg
     6361 cggtcgcgac gctcgcgatc atcccgttcg gcgacgtcgg cacctggggc ggaacgatcg
     6421 ggctgtacgg gatcgacgtc tcgatcggca ttctctacgc gttcgcgttc ggcgcgctcg
     6481 cgttctatgg cctgatgctc ggcggctggg cctccggctc gaagtactcg ttcctcgggg
     6541 cgatgcgctc ggccgcgcag ctgatctcgt acgagatcgc gctcggcctc tcgctgctcg
     6601 gcgtcgcgat ccaggcgggc tcgctgtcgc tcgtcgagat cgtgcacgcg caggacaact
     6661 actggttcat caccacgcag ttcgtcggct tcctgatctt cacggtcgcc ggcttcgcgg
     6721 agacgaaccg cgccccgttc gatcttcccg aggccgacgc ggagctggtc ggcggctaca
     6781 acaccgagtt cggcggcatg cgcttcggct cgttcttcat ggccgagtac atcaacatga
     6841 tcgtgatctc cgggatcgcg accgcgatgt tcctcggcgg ctggcgcggc cccggtcctg
     6901 aggccctcgg cccgctctgg gtcttgataa agatcttcct gttcctggtc ttcttcatct
     6961 gggtcagggc cacgctgccg cgcctccgct acgaccagct catgtcgttc ggctggaagg
     7021 tgctgctgcc tctcgcaacc gtgaacgtga tcgtgaccgc catcgtggtg gccacctgaa
     7081 gccgtgactc cctacaaccc ttccccagac atcatcgagg tccagcgcgg cccgaatccc
     7141 ggcgggctcg gcggcgcgta ccgaaccttc ggcgcgactc tgcgtgggct gaagaccacg
     7201 ctcggtcgcg tggtcgaggg cccggtcacg ctggagtacc cggagcagaa gacgccggtg
     7261 tatccgcgct tccgcggcaa gcacaagctc caccgcttcg aggacaccgg gctcgagaag
     7321 tgcgtgggct gctcgctctg cgccgccgcc tgtccggccg agtgcatccg tgtcgtcgcg
     7381 gccgagaaca ctccggatca ccaggtgtca gccggcgagc gctacgccgc ggtctacgag
     7441 atcaacatgc tgcgctgcat cttctgcggc tactgcgagc tcgcctgccc attcgacgcg
     7501 atcacgatgg gcaacgacta cgagctgtcc gactacacac gctctgacct catcttcacc
     7561 aaggagatgc tgctcgcgga ccatcccgag cggactccgc tgcggcgcga gggcgagtag
     7621 tggtcttcaa ggacatcgtc ttcttcatcg ccgcgatcgg cgcgctcacc ggcgcgatcg
     7681 gcgtggtgat gctgcgcaac ccgttcttca gcgtgctcgc gctggttggg cacctgttct
     7741 cgctggcggt gctgttcctg ctgctgtcgt cgcagttctt ggccgccgcg cagctcgtgg
     7801 tctacgcggg cgcggtgatg gtgctctacg tgttcgtggt cgcctacatt ggcggcgccg
     7861 acgagccgct cgggccgcag atccctggcg tcggcacgct cgggcccctc gtcgcggcgg
     7921 cgctgtgcgg cgagctgttc ttcgcgttca tcggcaccgg cctcgaggcg gtcgacagcc
     7981 ggggggcgaa gatcgggtcg ggcttcggct cgccgggtca gatcggcagc ctgctgctga
     8041 acaacttcct gatcccgttc gaggcagcct cgttcatcct gctcgtggcc gccgtgggcg
     8101 cggtggtgct cgcgcgccgg cggcgcggcc tcgatgagga cgagggcggt gcgcccgtgg
     8161 acgtgatcac gcacgaacgc ctgcccgaga cgcatccgca gcgcggcggc gtcacgtcgg
     8221 aggttggctc gtgaacctcg tctggtacct ctgcctgtcg gccgcgatct tctccgtggg
     8281 tgctgcgggc gtgctcatcc gccgcaaccc gctggtgatc ctgctctgcc tcgagctgat
     8341 gttgaacgcg ggcaacctcg ccttgatcac gttcgcgcgc atgcacggca acggtgacgg
     8401 tcagatcttc gccctcgtgg tgatggtggt cgccgcctgc gaagtggtgg tcggcctcgg
     8461 agtgatcgtg gccatgtacc gcaaccgcat cgccgaggat gtcgacgaga tgtcggagct
     8521 ccgagggtga gcgcgaccac ttgggggtgg ctggtccttg cgttcccgct cgccgggacg
     8581 attctgctgc cgctcgggtt caagttctgg ccgggccggc tgccgggctg gatcgggtcg
     8641 ctcacgatcc tgctctcgtt cggggcctcg ctcggcgcgc tgttcaacat catgggcgag
     8701 caccccgagc agcgctcgca ctacgacacg ctctggacct atgcgagcac cgcgaagttc
     8761 aacgcgggcc tgacgatcct ggtcgacccg ctgtcggtgt tcatgtgcct ggtggtgacg
     8821 ggcgtgtcgt tcctgatcca cgtgtactcg atctcctata tgggcggaga ccgcggctac
     8881 acgcgcttct tcacctacct gaactacttc gtcttctcga tgctcctcct ggtgctcggg
     8941 ggcaacatcg tgatcctgat catcggctgg gcgttcgtgg gcgccgcttc gtatctgctg
     9001 atctcgtttt ggtaccggcg ccgaacggcc accgcggccg gcatgaaggc gttcgtgatc
     9061 aacgtgatcg gcgacgtggg gctcgtcatc gccgccttcc tgctctggaa caagaccggc
     9121 gccctggact gggcgggcat cttcagcggg gcgaaggacg cgttcagctc gaacgacggc
     9181 acgctcatcg ccgcctgcct cttcctcacg atgggcgcgt tcgcgaagtc cgcgcaggtg
     9241 ccgctccaca cctggcttcc ggacgcgatg gaaggcccga cgcccgtctc ggcgctgatc
     9301 cacgccgcga cgatggtgac cgccggcgtc tacctcgtcg cacgcacgca cccgctctac
     9361 gagctcgcgc cgacggccgc cgacatcacc gccgcacttg ccacgttcac gctgctctac
     9421 gcggccacgt gcgcgctggc gatcaccgac ttcaagcgga tcatcgccta ctcaacgatc
     9481 tcgcagatcg gctacatgat gctcggcgtc gccaccttcg gctaccagtt cgggctgttc
     9541 ctgctgatgg cgcacgcctt cttcaaggca ctcctcttca tgggcgcggg ctcggtgatc
     9601 tcggccatgg gcggcgagca gaacatcgac cgcatgggcg gcttccggcg cgcaatgccg
     9661 ttcacctaca tcacgttcac gatcggcgcg ctggcgctcg cgggtctgcc catcttcgcg
     9721 ggctggctcg cgaaggacgg catcctcgga tggacgtttc accgcggcgg ctggtacatc
     9781 gcgctcggac tcgtgggcta cctcaccgcg ctgctaacgg cgttctactc gttccggatg
     9841 gtcttccggg tcttcttcgg cgagccggtg cgggaggcgc agttgctcga gggcggcgag
     9901 ctcgtgcacg gcgagcaccg caacccgctc agcggcgagg aggaggacac cgacgtcggc
     9961 ttccccggcc ccgagcacca catcgccgag cgcgaggcga cgatgaaggt agcgatgagc
    10021 ctgctcgcgg tgctctcgct cgcgggcggc atcgtcctga tcccgggcgt gaccgagtgg
    10081 ctccaggcgt tcctcgagcc gactttcagc gactccggtt tcgtcggctc gcacgtgccg
    10141 accggctccg agtggctcgg cctgggcgtc ggagcggtgg ccgcggccgc cggcatcgcg
    10201 gcggcctggt tcgcctatcg cgcccgcccc ggcatgacgc tcgagctgcg tgaccgcttc
    10261 gggcgcgtgc acgccttctt cttcaaccgc tggtacttcg acgagctgtt cgacgtcatc
    10321 ttcgtacggc ccacgctcgc ggttgcgcgc ttcttccaac gagtgatcga gcccggcgtg
    10381 gtccagggcg tgctggtcgg cggcgcgacc ggcgtcgtgc gcctgtcgac gaacttcgcg
    10441 cgctcgatcc agtccggcta cctgcgcgcc tacgccgggc tgctcgtcac cggtctgttc
    10501 ggactcgtcc tctacttcct gatccagagc tcctagccga tgcatctcac gatcccgctc
    10561 ttcatcccac tcgcggccgg catcgtcggg ctgctgctgc cgcgcgggct cgcgcgctgg
    10621 gcgtcgctcg cgggcacgct cgccgtgctc ggctactcgg tcgcgatgat cgtcgacttc
    10681 gactcggcca cgcgcgggct gcagtgggtc acgaacgaga cctggatcga cacgctcggg
    10741 atccactaca agctcggcgt cgacggcctg aacctgttcc tgatcctggc gaccgcgttc
    10801 ctgtgggtga tcgccactct gtacgcggcg gtgcgcgagt gggatcggcc gcggctcttc
    10861 tacttcaacc tcggcctcgc ggagaccgcc gtgctcggcg cctttctcgc ccaggacctg
    10921 gcgctgttcg tcgtcttctt cgatctcatg ctcgtgccgt tctacttcct gatcggcgcg
    10981 tggggacgtg gcaaccgcgt gtacgcgacc accaagttcg tgatctacac gctgtcgggc
    11041 tcgctgctga tgctcgcggg cgccgtcgcg ctcggagtgc tgtcgacgcc agagggcggc
    11101 cagatctcgt tccagctgtc cgatctcacc gtccatcccg tcggccacgg cacgcaggtg
    11161 tggatcttcc tgctgttcgc cgccgccttc ctgatcaagg cgccggcctt cccgctgcac
    11221 ggctgggtgc cggagaccta ccgtgccacg ccgatcccgg tgctgatcct gctctcggga
    11281 atcctctcga aggtcggcat gtacgccttc ctgcggatcg tgattccgat catgccgggc
    11341 gcctcggcga cgctgcagga gctgatgctc gtgatcgcgg tcgtctcgat cctgtacggg
    11401 tcggtgcttg cgttctctca ggacgacgca cggcttgtgg tcgcgtactc gtcgatcgcg
    11461 cagctgggtt tcatcctgct cgggatcttc gcgctcgatc ccaagagtgc gcaggggtcg
    11521 gtgatccaga tgctgaacca cgcagtggtg gtcgccgggc tgttcgtgat catcggcgtg
    11581 attgccgcgc gcgcccgcga gtccgagtcg ctcgagcgcc tgggcgggat ggcgctgcgc
    11641 gcgcccgtgc tcgccgggct gttcctgatc gtcgcgctgg ccacgctcgc tatgccgggc
    11701 tcgcccaact tcgtcggcga gatcctgatc ctgttcggca ccttcgacga gaagttcgtg
    11761 tacgggctcg tcgccagcgt gggagtcgta ctggctgccg tctacatgat ccggctctac
    11821 cagcgcacga tgcacaaccg cgccgggcct gacgtcgcgt cgagcgacct ctcgtaccgg
    11881 ctcgatctgc cggcgctcgc accggtggcg ctcgtgctga tcgcgctcgg cctctacccg
    11941 cagttcgtgc tccaccgcac ggaggcgtcc accactccga agatcgtgag ggcggcgatc
    12001 aaggccggct cggatcccgg gccccagcac gcggaggcaa ccaccgtgaa gggagccacg
    12061 ccgtgatcct cacgcttgcg gcgaaggtga cgccgccggt catcgactac aagggcttct
    12121 cgccgctgat cgcgctcgtc gggggcaccg tgctcgttct gctggtggcg ctcttccgcg
    12181 gccgcgcagt tcagcgtggc ctcgttccgc tgctcaccgc gggcacgctg ctcaccgcga
    12241 tgggcctgtc ggtctgggtc tggcacgccg gcgactccag gccgatcgtg gagggcgcta
    12301 tcgagcgcga cgcgctcgcg atgggcatgt cgatgatctt ctacgtggcg gggctcgcga
    12361 ccgtgctgct gtcgcaccgc tctgtggcgg tccgcgaagc gggcgcaggc gagtactaca
    12421 tgctgctgct ctcgtcggtg acgggcatgg tgctgctcgc gcaggccgcg aacctggtca
    12481 cgctcttcgt cgcgctcgag ctgctttcga tcccgctcta cgtgctctgc gcgagccacg
    12541 tgcgccggag gacctcgctc gaggccggcc tcaagtacct cgtgatcggc tcgctcggct
    12601 cggccacgct gctctacggc ctggcgatga tctacggcgc caccggcacg acctcgtttg
    12661 cgggcatcga caacgcgctc ggcgacacgg tctcgatcgc tgatccactc ttcctcaccg
    12721 gcgtgggact ggcgctcacg ggcttcgcat tcaaggcgtc ggtcgctccc ttccaccagt
    12781 ggacgcccga cgtttaccag ggcgccccca cgccgatcac ggcgttcatg gccgtggcga
    12841 cgaaggcggc cgccttcgcg atgttcctgc gcgtgctcga ccaggcgctg ccgtcggcgc
    12901 agacctactg ggcgcccgcg ctcgcgacgc tcgcggtgat cacgatcatc gtcggcaacg
    12961 tcggcgcgat cgcacagagc agcctcaagc ggatgctcgc gtggtccggc gtggcgcagg
    13021 cgggctacat cgccgcaggc gtggtcgtcg gcacacagaa gggactcgag gcgaccgcct
    13081 tctacctgtt cgtgtacctg ctgatgaacg tcggggcgtt cgcggtgatc gtggcgcgcg
    13141 agcgggtctc cgagttcggc gacgacatcc gctcgctcga agggctgggt cgcgcggcgc
    13201 cgtggctcgc gtggccgatg accatctgca tgctggcgct cgcgggcttc ccggccacgg
    13261 gcggcttcat cggcaagttc tatttgatcg acgcggccgt gagcggcgac tacgcatggc
    13321 tcggaatcgt gatcgtggcg ggttcggtga tctcgctcgt ctattacctg cgcgtgatcg
    13381 cggtgatgtt catgggcccg ttcgagatca cgctgcccgc gatgcccggg cgccccgcgc
    13441 gcagggtgaa gccggtgggc ggatggtcgc ccgaggccga cgcctggcgc cagcccgagg
    13501 tggtgatcgt cgcgttgctg tgcgcggcag ccacgatctt cttcggcgtg tggccgaacc
    13561 cgctgttcga cgtggtgcgc gacatgggca cgtcgatcca gtcgctgatc ggctgaggcg
    13621 accgtgagcc tgcgcgtgcg ctgggtgatc gcggcgctgg tcggcgctgc gctgataggc
    13681 gggctcatct gggcctacag cggcgacgag agcttcacgc gtgcgacagc cgaagaccag
    13741 ctgcagcagt tccacgacga gcggcgcccg aacacgccag ccgagaacgt gcgctgcgag
    13801 accgcgggca gcgcgtggcg ctgccgcttc cgcgaggacg gcaaggagtg cgccggcgcg
    13861 gtgagcggca agccgacgtc gcc
//
LOCUS       DASVXS010000232        14754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_985594, whole genome shotgun sequence.
ACCESSION   DASVXS010000232 DASVXS010000000
VERSION     DASVXS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14754
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985594"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1088)
                     /locus_tag="VF752_13050"
     CDS             complement(93..1088)
                     /locus_tag="VF752_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8856516.1"
                     /translation="MSTVREAVFEMLRERGMTTVFGNPGSTELPMLKDFPDDFRYVLG
                     LQEAVAVGMADGFAQASGIPTQVNLHTAPGVGNGMGAIFNAQANKSPLLVTAGQQARS
                     LMTLQANLTNRDATRMPHPLVKWSYEPPRAEDVPHAFARAAHLASLPPKGPVFVSIPM
                     DDWDKEVDDDAVRHQTTRKVVGRAAGDPGAVRELASRLSSAENPVLVVGPDVDASGAW
                     DAAVALAEKQRLPVWAIPAPGGGRIGFPEDHPAFQGILAPAIGPLSEMLRAHDFILVA
                     GSSVFPYYPNIPGPLLPEGASLVAITSDPDEAARAPMGDAIVADGDSITVIASSG"
     gene            1164..1790
                     /locus_tag="VF752_13055"
     CDS             1164..1790
                     /locus_tag="VF752_13055"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856517.1"
                     /translation="MPKPITEIAPGLYHWTARHPRIGMDVSSYWMPEERVLIDPLLPE
                     EGIEWFESDGGGAPRTALLSNRHHWRSCSDLIERYGVTVRASRPGMHEFGPDQPVEPF
                     DFGDEIAPGVIGYEVGGICPDESALHSSARGTLSVADGIVRWREGGELAFVPDNMLDD
                     PEQDKAAMLTAYRRLLDLDFDHVLLAHGDPAVGDGREKLREFVEREGG"
     gene            complement(1787..2413)
                     /locus_tag="VF752_13060"
     CDS             complement(1787..2413)
                     /locus_tag="VF752_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856518.1"
                     /translation="MIEKIQNQAAAHAHSAGAKASATGGADFATLHKKALAKASASNP
                     QQGADGQPTEGATPGLNTKLKPPKGETWGPVEGHPEYADILSGPRNGFYVNLAPGKRQ
                     GQTFVIVHRDGKTFHVYGEGKNRKSVEVRETPKPLAGEQWQQVGAHRDYKKIDGGKRD
                     GEYVNLSGNERQGRTFEIVERDGKRYHAYGTGKDRLLVHVGWHRESGS"
     gene            complement(2472..3014)
                     /locus_tag="VF752_13065"
     CDS             complement(2472..3014)
                     /locus_tag="VF752_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856519.1"
                     /translation="MKLLGAVGAVAALLVVATVLLLGGGDVKRDPASAGKTIVDKSHR
                     FALSYPASGWNAVPASGLAKVPSRPAAVLQRADRRATVVVRSRAALNSGLVQIARGLT
                     PELKRRFSDFHPLSARIVRLAGGPALAYMFAGTRTGTVQTELVAPAQGRSFTVEAVAR
                     GNDPRVAREVGAILRSFVAR"
     gene            complement(3014..3169)
                     /locus_tag="VF752_13070"
     CDS             complement(3014..3169)
                     /locus_tag="VF752_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856520.1"
                     /translation="MILNVDENLEDGLMAQAVPPTQFDVLKIRLCNTTANPIVASARS
                     YSYVVVR"
     gene            complement(3166..4533)
                     /locus_tag="VF752_13075"
     CDS             complement(3166..4533)
                     /locus_tag="VF752_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856521.1"
                     /translation="MGDAAVDHVGVTRARLVVPLFALLLATPAPALAAKKKNKANVDV
                     VTMVRRAFHIASRADAAARKAVTVAANAGVKDGEVTTSKLADGAVANAKLADAAVTAA
                     KIADSAVTSAQLGGGAVAFADLAADSVRGSNILDGTIGSNDLGLDAITASKIADGSIG
                     NSKLATDAVTSGKIANGTVVSADIGTGEVKTTNILDGDVGTSDLATDAVTSSKIFDGT
                     IGSADIGTGQVTTANILNGDVGTADLATDAVTSGKIFDGTIASADIGTDQVTTANILN
                     GDVGNADLATDAVTSGKIFDGTIASIDIGTGQVTTANILNGDVGNADLGLDAVTSAKI
                     LDGTITSADIGPGQIAASRLAPNSIDQTVLATGSVDSTILAPDSVINSKIGDYAVDTL
                     QLANNAVTTAKIGNLQVDGTKLATDAVTSTKVKDGSLTAADIAAPDNGTGVLVGSVSI
                     NPVTT"
     gene            4563..5312
                     /locus_tag="VF752_13080"
     CDS             4563..5312
                     /locus_tag="VF752_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8856522.1"
                     /translation="MPVKLYVIPASHPSRAAELMLERKGIPFNRVDLVSALHKPVLRA
                     LGFPHATVPAMKLDGRRIQGSRAIARALDIERPDPPLLPADPALRARVEEAECFGDEQ
                     LQGVPRRLAWWALKRDPSGVRSFLEGSKLPMPTSVAAATSGPIIAVAVRLNGATDEAV
                     HADLAGLPGMLDRVDSFIEEGVIGGTEPNAADYQIATSVRLLMCFDDLRPLIETRPAG
                     KHALALVPDFPGRMPPVFTPEQLALLGAEAA"
     gene            complement(5292..6734)
                     /locus_tag="VF752_13085"
     CDS             complement(5292..6734)
                     /locus_tag="VF752_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX8856523.1"
                     /translation="MEALRRRNTRALAAAGLALAALVAGLVVGLGHNGSEKRLVPAAG
                     GQDDTYDPLAYDESRRGQFEQQAANGLSHVLYAKSPGGVTATAQRVARFRPLVTKAAA
                     AGKVDPDTLEAIVFLESAGRPDVIAGGNPENASGLTQILAQTGSGLLAMHVDLAKSRK
                     LTKKIAHAHGKRLKILLRQRRHADDRFDPAKALAGTVRYLQFARTKLERDDLAVVSYH
                     MGVGNLQKALSFYGSSDIPYAQLYFDSTPLVHRRAYQLLASLGDDSSTYYWRVLAAKQ
                     IMRLYRTNPSELKRVSALQNGRSNAELVLHPPETTRSYDTPKELRSGYLLGPLKRLPN
                     NPSRLAFKVDPSMGSLAKRVHAPRALYRGLRPEALALLAYMGAGTRAIAGGGSLVVTS
                     SVRDRQYQRALMAGNIEATHGYSLHTTGFAFDVARLYSSRRQALAFQFMLDRLTALNL
                     IAWAREPQAIHITVSSAAKKLEPVLGSLGA"
     gene            6892..7890
                     /locus_tag="VF752_13090"
     CDS             6892..7890
                     /locus_tag="VF752_13090"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8856524.1"
                     /translation="MEIVPAYREKSPSRPERPRRHGRNSRPDERDRRAAAIASRRHGT
                     LTSEELREVGFSASAVTRAVQRGAIQRLYHSIYVMGPVIPPLARELGAVRACEPDAFL
                     GSLSALMLWDLVEDYHGPIHVTVVGRDCRRQDGIKVHRVNALDRRDTRRHKLIPATAP
                     ARAILESAAELSPARLRRLVDNATVRRLTSEAQLRQAMKRAPRRAGTAALDAILTANR
                     EPALTRSEAEERALALIEAAGLPRPKVNSRVAGLEVDFYWPEHKVVLEIDGFEFHRTR
                     HAFENDHERTARLEDAGLVVRRATWLQLTEEPLRLTARLARALAAGEGAHGKRAAA"
     gene            complement(7887..8627)
                     /locus_tag="VF752_13095"
     CDS             complement(7887..8627)
                     /locus_tag="VF752_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856525.1"
                     /translation="MRLLRRIAITAVIAGVLSAVVSTALYGNPVALAASTSTSLHDVL
                     SQLRRNSKVSERLNAGFFRAPQTRAFVGSAFQPTNSNVEYIADRGDSSLSLAPKRPTT
                     DTETNNGTTTVKTVDQTTGTFEYALDIPNRAQVVKVTASYRDATGGNTTTNGQAASSG
                     MNFQVLELGQLGDSPKELLSSATGVRSRDGQAETLNLPIAGNGFRVNNSLKRYVLRVT
                     LDDVQPDTRFYGVTVQYVIGKGVAGAPS"
     gene            complement(8718..8984)
                     /locus_tag="VF752_13100"
     CDS             complement(8718..8984)
                     /locus_tag="VF752_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8856526.1"
                     /translation="MDLLDLLGRRWTLRVLWELRDGSEYGFRQLQDRCGGMSSSVLAT
                     RLAELRDAGIVETSDSGAQKLTPEGSELVERLIPLNEWAQRWGA"
     gene            9072..9632
                     /locus_tag="VF752_13105"
     CDS             9072..9632
                     /locus_tag="VF752_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8856527.1"
                     /translation="MAATRIAPVEPPYEPEVEEILKRWTPGGYDVDPPAIFRLYQRHP
                     ELASRLRPLGGYVLGHALIGDRARELVILRTTARAGAEYEWGVHAATLARDAGLTAEQ
                     IAATAKGTAGDPAFDERDSLIIALCDELHDTATVSDGLWSTLAEHWDDRELLELVVIV
                     GWYRLHSYVLNAARLELEPWGERFPK"
     gene            9629..10273
                     /locus_tag="VF752_13110"
     CDS             9629..10273
                     /locus_tag="VF752_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8856528.1"
                     /translation="MSLWGRIFALGYEPFQALSERAGTRALRRRLLEQASGRVLEVGA
                     GTGLNLPHYTEQVSELVLLEPEEPMAHQLERHLGRKRARTATVVRAPGEELPFEDDSF
                     DYVVTTFVLCTVDDPERTLAEFDRVLKPGGRFAFLEHVRAEDGTSLAGWQDRLHGAWL
                     RFGQGCHCNRRSEQAIAASPLSLERVERGMLPKEPPIVKPYVVGTAFSEPRSIG"
     gene            complement(10228..11634)
                     /locus_tag="VF752_13115"
     CDS             complement(10228..11634)
                     /locus_tag="VF752_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8856529.1"
                     /translation="MIFRQITHDDLGCASYFIGDEDAGVAAVVDPRFEINEYLELARY
                     MGVHIDHVFETHNHADHVSGHGRLAAATGAEIHVHREAGVAYDHVPFDDGDELELGAL
                     RVRALHTPGHRPEHTAFALVDTARGDEPWAVLTGDTLFVGDIARPDLAVDKEEGAHGI
                     FHSLHEKLLALADETEVWPGHLGGSLCGGPGMDLKIASTIGYERRHNDMLRVPEEDEF
                     VRRSVASLGPQPPNFQAIVDLNRGPLVTAGVEVGPLTPRQVDMKRQDGALIVDVRTDL
                     QFDDAHVPRSVCNTMLRAGFGTKLAWIADRDQEIVFVGRDDEDGRRAARLSVAVGIRK
                     LGGFLAGGFTSWRMEHGEVESVDRVGVDELRARMESGEDVQILDVREASEWREKHIPD
                     SVFVPYHDIHSVPEGLDPSRPVAAMCSSGQRSAVAASLLKRYGLTDVWHVVNGGVGTW
                     ERAGYPIERGSEKAVPTT"
     gene            11670..11882
                     /locus_tag="VF752_13120"
     CDS             11670..11882
                     /locus_tag="VF752_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856530.1"
                     /translation="MKLEQPRAGVFQATMTAHELSVLLAGARMSLNVMQAGEDVATAE
                     ASQALETVLADFDTAIKRATAERADG"
     gene            complement(11904..12311)
                     /locus_tag="VF752_13125"
     CDS             complement(11904..12311)
                     /locus_tag="VF752_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8856531.1"
                     /translation="MTRAEVDRDIQETFGLVPEFLKTVPDYLISSEWQSFKALELAET
                     AIPNKYKELIGLAVSGATRCRYCAYFHTEAAKLFGATDEEVNEACLIAKNTMGWSTYL
                     NAMQFDYDEFKEEFDRSVEYVREQMAAQEPAHA"
     gene            complement(12498..13154)
                     /locus_tag="VF752_13130"
     CDS             complement(12498..13154)
                     /locus_tag="VF752_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8856532.1"
                     /translation="MTDTSPTRAIANHTEARGRLTGAERRAAILDAAMEVFARRGFHG
                     SSIDEIAKAAGISKALIYEHFPSKKDLHNSLLEHQVDELLGRLDASAATGDAGDMRLR
                     AGVEAFLGFVEERREAWRMLFRDSADPDLAETFERVQLRATAAIADLIARDPGASAAP
                     EASFNIEMLAQQLSGSVQSLANWWYVHQDVPREEVVSRVMDFAWVGLGRLSQGESYRG
                     "
     gene            complement(13203..14327)
                     /locus_tag="VF752_13135"
     CDS             complement(13203..14327)
                     /locus_tag="VF752_13135"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX8856533.1"
                     /translation="MTQQRVDLESEGLLEGVEGDARESRRELLTTLLDEGVPLDELKQ
                     AVEEDRLVLLPVERELLGSGERLTGRQVAERAGLDLEFWERLGRSLGLAVADPDEAIY
                     GEFDLEGARRVKRFLDAGFPEQTILDVNRVMGQSLGQIARALGDVTTSTLLESGASER
                     EVALGLAAAQRELGPVFGQTMEYVLRRQQLEVARQGAVTGAEIAAGRPAGARDVSVAF
                     ADLVGFTRLGEELPPEDVGSLADRLNDMTLEVTEPPVRLVKMIGDAAMLASPDSDALL
                     GAALALLGAAEAEGDGFPQLRVGVACGPALARAGDLYGRPVNLASRVTAIARPGSALT
                     TAEVKEDAEGDYHWTHVGRRRVKGVRGGVDLYRVRRPSGD"
     gene            14397..>14754
                     /locus_tag="VF752_13140"
     CDS             14397..>14754
                     /locus_tag="VF752_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8856534.1"
                     /translation="MEGARVPTADTPDRLPRGPHRLAREVVLASQRGRMLDAMAQSVA
                     EKGYAATTVADVVGRAGVSRKTFYEHFRDKEDCFLEAWDAGVEILLDAVAQATTATED
                     WREGVRAGTRAYLETLA"
BASE COUNT         2140 a         5083 c         5206 g         2325 t
ORIGIN      
        1 cgcgacgcgc cgccggcccg cccggcgccc gagcccgtcg agctcaccga cccgatgagc
       61 ccgagcacgg cgatggacat cctcggcgag ctctaccccg aggacgcgat cacggtgatc
      121 gagtcgccgt cggcgacgat cgcgtcgccc atcggcgcgc gcgccgcctc gtcggggtcg
      181 ctcgtgatcg ctacgaggga cgcgccctcg ggcagcagcg gcccggggat gttcgggtaa
      241 taggggaaca ccgacgagcc ggccacgagg atgaagtcgt gcgctcggag catctccgac
      301 agcgggccga tcgccggcgc gaggatgccc tggaacgccg ggtggtcctc cgggaacccg
      361 atccggccgc cgcccggcgc cgggatggcc cacacgggca ggcgctgctt ttcggcgagc
      421 gccacggcgg cgtcccaggc gccgctcgcg tccacgtccg ggccgaccac cagcaccgga
      481 ttctccgcgg acgacaggcg cgacgccagc tctcgcaccg cgcccgggtc accggccgcg
      541 cggccgacca ccttgcgggt ggtctggtgg cgcacggcgt cgtcgtccac ctccttgtcc
      601 cagtcgtcca tcgggatcga gacgaacacg ggacctttcg gcggaagtga cgccaggtgc
      661 gcggcgcgcg cgaaggcgtg cggcacgtcc tcggcgcgcg gcggctcgta gctccacttc
      721 acgagcgggt gaggcatgcg cgtggcgtcg cggttcgtga ggttggcctg cagcgtcatc
      781 agcgagcggg cctgctgtcc ggccgtcacg aggagcggcg acttgttcgc ctgggcgttg
      841 aagatcgcgc ccatgccgtt gccaacgccc ggagccgtgt gcaggttcac ctgtgtgggg
      901 atgccgctcg cctgcgcgaa gccgtcggcc attcccaccg ccaccgcctc ctgaagcccg
      961 agcacgtagc ggaagtcgtc ggggaagtcc ttgagcatcg gaagctcggt ggaccccggg
     1021 ttcccgaaca cggtggtcat gccgcgttcg cggagcatct cgaagacggc ttcacggacg
     1081 gtcgacactc ggctgcctcc tggcggttgg acgggcgcgg gagcttatcc gcgaaacatc
     1141 cgcgacgcgg tagggtcgcc gccatgccaa agccaatcac cgagatcgca cctggcctct
     1201 atcactggac cgcgcgccac ccgcgcatcg gcatggacgt gagctcctac tggatgcccg
     1261 aggaacgcgt gctgatcgat ccgctcctgc ctgaggaggg catcgagtgg ttcgagtcgg
     1321 acggcggcgg cgcgccgcgc accgcgctgc tcagcaaccg tcatcactgg cggagctgca
     1381 gcgatctcat cgagcgttac ggggtcaccg tgagggcgag ccgtccgggt atgcacgagt
     1441 tcggcccgga ccagccggtc gagcccttcg acttcggcga cgaaatcgct cccggcgtga
     1501 tcggttacga ggtcggcggc atctgcccgg acgagagtgc gctccactcg agcgcgcgcg
     1561 gcacgctgtc ggtcgccgac ggcatcgtgc gctggcggga gggcggcgag ctcgccttcg
     1621 tgcccgacaa catgctggac gacccggagc aggacaaggc ggccatgctc acggcgtacc
     1681 gcaggctgct cgacctcgac ttcgaccacg tgctgctcgc gcacggcgat ccagcggtcg
     1741 gcgacgggcg cgagaagctg cgcgagttcg tggagcgcga gggcggctag ctgccgctct
     1801 cgcggtgcca gccgacgtgg acgagcaggc ggtccttgcc ggtgccgtag gcgtggtagc
     1861 gcttgccgtc cctctcgacg atctcgaagg tgcggccctg gcgctcgttg cctgagaggt
     1921 tcacgtactc gccgtcgcgt ttgccgccgt cgatcttctt gtagtcgcgg tgggcgccca
     1981 cctgctgcca ctgctcgccg gcgagcggct tcggcgtctc gcgcacctcc accgacttgc
     2041 ggttcttgcc ctcgccgtag acgtggaagg tcttgccgtc cctgtggacg atcacgaacg
     2101 tctggccctg gcgcttgccg ggggcgaggt tcacgtagaa gccgttcctt ggaccactga
     2161 ggatgtcggc gtactcgggg tggccctcca cagggcccca ggtctcgccc ttcggcggct
     2221 tgagcttcgt gttcagcccc ggcgtggcgc cctcggtggg ctggccgtcc gcgccctgct
     2281 gcgggttcga cgcggacgcc ttcgcgagcg ccttcttgtg cagggtcgcg aagtcggcgc
     2341 cgccggtggc ggacgccttg gcgccggcgc tatgggcatg cgccgcggcc tggttttgga
     2401 tcttctcgat catgcgagcg ggtgagaggg attgcgctcg tcccgggtgg tcggccgcgg
     2461 gtcgcgaagc tttagcgcgc cacgaaggag cgcaagatcg cgcccacctc gcgggcgacg
     2521 cggggatcgt tgccgcgcgc caccgcctcg acggtgaagc tgcggccctg ggcgggcgcg
     2581 acgagctcgg tctgcaccgt gccggtgcgc gtgcccgcga acatgtaggc gagcgcgggg
     2641 ccgcccgcta ggcgcacgat gcgcgcgctc agggggtgga aatcgctgaa gcggcgcttc
     2701 agctcgggcg tgagcccgcg cgcgatctgc acgaggccgc tgttgagcgc ggcgcggctg
     2761 cgcacgacca cagtcgcccg gcggtccgcg cgctggagca ccgcggcggg gcggcttggc
     2821 accttcgcga ggccggacgc cggcacggcg ttccagccgc tggccgggta gctgagcgcg
     2881 aagcggtgcg acttgtcgac gatcgtcttg cccgcgctcg cgggatcgcg cttcacgtcg
     2941 ccgcctccga gcagcagcac ggtcgcgacc accagcagcg cggcgaccgc tcccaccgct
     3001 ccgagcagct tcatcagcgc accaccacgt agctgtagga gcgcgccgag gccacgatcg
     3061 ggttcgcggt ggtgttgcag aggcggatct tcagcacgtc gaactgcgtt ggcggaacgg
     3121 cctgcgccat gaggccgtcc tcgaggttct cgtccacgtt gaggatcacg tggtcacggg
     3181 gttgatcgac accgagccga cgagcacgcc cgtgccgttg tcgggtgccg cgatgtcggc
     3241 ggctgtgagc gagccgtcct tcaccttggt gctcgtgacg gcgtcggtgg ccagcttcgt
     3301 gccgtccacc tgcaggttcc caatcttcgc cgtggtgacc gcgttgttcg cgagctgcag
     3361 cgtgtcgacc gcgtagtcgc cgatcttcga gttgatcacg gagtcgggcg cgaggatggt
     3421 gctgtcgact gagccggtcg cgagaacggt ctggtcaatc gagttgggcg cgagccgcga
     3481 ggcggcgatc tggcccggac cgatatccgc ggaggtgata gtgccgtcga ggatcttggc
     3541 gctcgtgacc gcgtcgaggc cgaggtcggc gttgcccacg tcgccattga ggatgttcgc
     3601 ggtggtcacc tggccggtgc cgatgtcgat cgaggcgatc gtgccgtcga agatcttgcc
     3661 gctggtgacc gcatcggtcg cgaggtcggc gttgccgacg tcgccgttga ggatgttggc
     3721 ggtggtcacc tggtcggtgc cgatgtcggc cgaggcgatc gtgccgtcga agatcttgcc
     3781 gctcgtcacc gcgtcggtcg cgaggtcggc ggtgccgaca tcgccgttca ggatgttggc
     3841 tgtggtgact tgaccggtgc cgatgtcggc cgagccgatg gtgccgtcga agatcttgct
     3901 cgaggtgacg gcgtcggtcg cgaggtcgga ggtgccgacg tcgccgtcga ggatgttggt
     3961 ggtcttcacc tcgccggtgc cgatgtcggc ggagaccacg gtgccgttgg cgatcttgcc
     4021 gctcgtgacc gcgtccgtgg cgagcttcga gttgccgatc gagccgtcgg cgatcttcga
     4081 ggcggtgatc gcgtcgagtc ccaggtcgtt cgagccgatc gtgccgtcga gaatgttgga
     4141 tccgcgcacc gagtcggcgg cgagatccgc gaacgcgacc gcgccacccc cgagctgggc
     4201 gctcgtcacc gcgctgtcgg cgatcttggc cgcggtcacg gccgcgtcgg ccagcttggc
     4261 gttcgcgacg gcgccgtcgg cgagcttcga ggtcgtcacc tcgccgtcct tcacacccgc
     4321 gttcgcggcc acagttacgg ccttgcgcgc ggcggcatcg gcacgggacg cgatgtggaa
     4381 cgcgcgacgc accatcgtca ccacgtccac gttcgccttg ttcttcttct tcgccgcgag
     4441 cgcaggggcg ggcgtcgcca ggagtagcgc gaaaagggga acgactaggc gtgcacgtgt
     4501 cacgcccacg tgatcgacag ctgcatcccc cacttgaggg tgtcgcgtac gatgcgcgcg
     4561 tcgtgcccgt gaagctctac gtgatcccgg cgtcgcatcc gtcgagagcg gccgagctca
     4621 tgctcgaacg caagggcatt ccgttcaatc gcgtggacct cgtctcggcg ctgcacaagc
     4681 cggtgctgcg cgcgctcggg ttcccgcatg cgacggtgcc ggcgatgaag ctcgacgggc
     4741 ggcgcatcca gggctcgcgg gcgatcgcgc gggcgctcga catcgagcga ccggatccac
     4801 cgctgcttcc cgcggatcct gcattgcggg cgcgcgtgga ggaggcggag tgcttcggcg
     4861 acgagcagct gcagggtgtg ccgcgtcggc tcgcctggtg ggcgctcaag cgcgatccct
     4921 cgggggtgcg cagcttcctc gagggctcga agctgccgat gccgacctcg gtcgcggccg
     4981 ccacgtcggg gccgatcatc gcggtggcgg tgcgcctcaa cggcgcgacc gacgaggcgg
     5041 ttcacgcgga cctcgccggg ctgcccggca tgctcgaccg ggttgactcc ttcatcgagg
     5101 agggcgtgat cgggggcacc gagccgaacg cggccgacta ccagatcgcg accagcgtca
     5161 ggctgctcat gtgtttcgac gacctgcgcc cgctgatcga gactcgtccc gcgggcaagc
     5221 atgcgctcgc gctcgtgccc gacttcccgg gccggatgcc gccggtgttc acgccggagc
     5281 agctagcgct gctaggcgcc gaggctgcct agcaccggct cgagcttctt cgccgcactt
     5341 gacacggtga tgtggatcgc ctgcggctcg cgcgcccagg cgatcaggtt gagcgccgtg
     5401 agccggtcga gcatgaactg gaacgcgagg gcctggcgcc tgctgctgta gaggcgcgcg
     5461 acgtcgaagg cgaagccggt cgtgtgcagc gagtacccgt gcgtggcctc gatgttcccg
     5521 gccatcagcg cgcgctggta ctggcggtcg cgcacgctgc tcgtgacgac gagcgagccg
     5581 ccgcccgcga tcgcgcgcgt gccggcgccc atgtaggcga gcagcgcgag cgcttccggg
     5641 cgcaggccgc ggtagagcgc gcgcggggcg tgcacgcgct tcgccagcga gcccatgctc
     5701 gggtcgacct tgaacgccaa gcggctcggg ttgttcggaa gcctcttgag cgggcctagg
     5761 aggtagcccg agcggagttc cttcggggtg tcgtagctgc gcgtggtctc gggcgggtgg
     5821 aggacaagct cggcgttcga gcgaccgttc tgcagcgccg agacccgctt cagctcggag
     5881 ggattggtgc ggtacaggcg catgatctgc ttcgcggcga gaacgcgcca gtagtaggtg
     5941 gagctgtcgt cgccgagcga ggcgagcagc tggtacgcgc gccggtgcac gagtggtgtg
     6001 gagtcgaagt agagctgcgc gtaagggatg tcgctgctgc cgtagaacga cagcgccttc
     6061 tgcaggttgc cgacgcccat gtggtacgag accacggcga ggtcgtcgcg ctcgagcttc
     6121 gtgcgcgcga actgcaggta ccggaccgtg cccgcgagcg ccttcgccgg gtcgaagcgg
     6181 tcgtcggcgt ggcggcgctg ccggagcagg atcttcagtc gcttcccgtg ggcgtgcgcg
     6241 atcttcttcg tgagcttgcg gctcttcgcg aggtccacgt gcatggccag gaggccactg
     6301 ccggtctgag cgaggatctg ggtgagcccg ctggcgttct cgggattgcc gccggcgatc
     6361 acgtccggcc gcccggcgct ctcgaggaaa acgatcgcct cgagcgtgtc ggggtcgacc
     6421 ttcccggcgg ctgcggcttt ggtgacgagt gggcggaagc gggcgactcg ctgcgcggtc
     6481 gcggtcactc cgcccgggct cttcgcgtag agcacatgcg agagaccgtt tgccgcctgc
     6541 tgctcgaact gcccacgcct gctctcgtcg tacgcgagcg ggtcgtaggt gtcgtcctgc
     6601 ccgccggctg cgggcacgag ccgcttctcg ctgccgttgt gaccgagccc gaccacgagt
     6661 ccggcgacca gcgcggcaag agcaaggccg gcggcagcga gcgcacgggt gttccgtcgc
     6721 ctgagcgcct ccacagggcc gcacgatagt gaccgctgcg taacgactgg ctaacggctc
     6781 cgtgaaaacc ggccgcggcg gtccccgagc ggcgctttgg tgcggcatgg tggccgagtg
     6841 gcccctggag agcggcaaaa agttccatct cacgcgttcg gcggagaagc gatggagatt
     6901 gttcccgcat atcgggagaa atctccatcc cgacctgagc gaccgcgtcg gcatggaaga
     6961 aattcccgcc ccgatgagcg ggaccggcga gcggcggcga ttgcgtcgcg ccgccacggg
     7021 acgctcacga gcgaagagct gcgggaagtc gggttcagcg ccagcgcagt gacgcgcgcg
     7081 gtgcagaggg gagcgattca acgcctctac cacagcatct acgtcatggg ccccgtgatt
     7141 ccgccgctcg cacgtgagct cggagcggtt cgtgcctgcg agccagacgc cttcctgggc
     7201 agcctctcgg cactcatgct gtgggaccta gtcgaggact accacggccc gatacacgtc
     7261 acggtcgtgg gtagggactg ccggcgccag gacggcatca aggtgcaccg agtgaacgcg
     7321 cttgaccgcc gcgacacgcg ccggcacaag ctgatcccgg ccactgcccc ggcgcgcgcg
     7381 atcctggaga gcgcagccga gttgagccca gcgcgcctac gccgtctcgt ggacaacgcg
     7441 accgtgaggc gcctgaccag cgaggcgcag ctgagacagg cgatgaagag agccccacgc
     7501 cgggcgggaa cagctgcact ggacgccatc ctcactgcca accgggaacc agcactaacc
     7561 aggagcgaag cagaggaacg cgcactcgct ctcatagaag ccgccggcct accacgccct
     7621 aaggtgaact cccgcgtagc gggcctcgag gtcgacttct actggcccga gcacaaggtc
     7681 gtcctcgaaa tcgacgggtt cgagttccac cgaacgcggc acgcgttcga gaacgatcac
     7741 gaaaggacgg cgcgactcga ggacgcgggc ctggtcgttc gtcgcgccac ctggctccaa
     7801 ctgacggaag agccgctccg tctcacagcg cgcctcgcgc gcgccctcgc cgcgggcgag
     7861 ggcgcgcacg ggaaacgagc agcggcctag gacggcgccc cggccactcc cttgccgatc
     7921 acgtactgca ccgtgacccc gtagaagcgc gtgtcgggct gcacgtcgtc caacgtgacc
     7981 cgcagtacgt agcgcttcag gctgttgttc acccggaagc cgttgccggc gatcgggagg
     8041 ttgagcgtct ccgcctggcc gtcgcgcgag cgcactcccg tcgcgctcga gagcagctcc
     8101 ttcggcgagt cgccgagctg gccgagctcg agcacctgga agttcatccc gctcgatgcc
     8161 gcctggccgt tcgtcgtggt gttgccgccc gtcgcgtcgc ggtacgaggc ggtcaccttg
     8221 accacctgcg cgcggttcgg gatgtcgagc gcgtactcga acgtaccggt ggtctggtcg
     8281 acggtcttga cggtcgtcgt gccgttgttc gtctcggtgt ccgtcgtcgg acgcttcggc
     8341 gcgagcgaga gtgacgagtc gccccggtcg gcgatgtact ccacgttcga gttcgtcggc
     8401 tggaaggccg agcccacgaa cgcccgcgtc tgcggcgcgc ggaagaagcc ggcgttcagg
     8461 cgctccgaga ccttcgagtt acggcgaagc tgagacagca cgtcgtgcag cgaagtcgac
     8521 gtcgacgcgg cgagcgcgac ggggttcccg tacagcgcgg tgctcacgac ggcgctcagc
     8581 acaccggcga tcacagcggt aatggcgatt cggcgtagaa gacgcacgaa acctccttgg
     8641 tcgtgtccct gtctgccctc tcggaacacg acctcgcgcg ccagttgcgc ctgacacgga
     8701 gatttcagtc agcgccctta cgcgccccaa cgctgcgccc actcattgag cgggatgagc
     8761 cgctcaacca gctcggaccc ctcgggcgtg agcttctgcg cgccagagtc actcgtctca
     8821 acgatgcccg cgtcgcgcag ctccgcgagg cgtgtggcga gcacgctcga cgacattccg
     8881 ccacagcgat cctgcagttg gcggaacccg tattcggacc cgtcgcgcaa ctcccacagc
     8941 acgcgcagcg tccagcgccg cccgagcaga tcgagcagat ccatgaccgg tcggctgtgt
     9001 cgccgtcttg actgcttcgg attcagaagc taggctcccc gcttcgaata acgaagcaag
     9061 gagcgtacac gatggccgcc acccgcatcg cacccgtcga gccgccttac gagccggaag
     9121 tcgaggagat cctcaagcgc tggaccccgg gtggctacga cgtcgacccc cctgcgatct
     9181 tccgcctcta ccagcgccat cccgagctgg cctcgcggct gcgcccgctc ggcggctacg
     9241 tgctcggcca cgcgctgatc ggcgatcgtg cgcgcgaact cgtgatcctg cgtaccactg
     9301 cccgcgcggg cgccgagtac gagtggggcg tgcacgcggc gactctcgcg cgagatgccg
     9361 ggctgaccgc cgagcagata gcggcgaccg cgaagggcac cgcaggcgac cccgccttcg
     9421 acgagcgcga ctcgctgatc atcgccctgt gcgacgagct gcacgacacc gccacggtct
     9481 cggacgggct ctggtcgacg ctcgccgagc actgggacga tcgcgagctg ctcgagctcg
     9541 tcgtgatcgt gggctggtac cgcctccact cctacgtgct gaacgccgcg cgtttggagc
     9601 tcgagccgtg gggagaacgc tttcccaagt gagtctctgg ggccgcatat tcgccctcgg
     9661 ctacgagccg ttccaggcgc tgtcggagcg agccggcacg cgcgcgctca ggcgccgcct
     9721 gctcgagcag gcgagcgggc gggtgctcga ggtgggcgcg ggcactggcc tcaacctgcc
     9781 ccactacacc gagcaggtga gcgagctcgt cctgctcgag cccgaggagc cgatggcgca
     9841 ccaactcgag cgtcacctcg ggcgaaagcg cgctcggacg gccacggtcg tgcgcgcgcc
     9901 cggcgaggag ttgccgttcg aggacgacag cttcgattac gtggtgacca cgttcgtgct
     9961 ctgtacggtc gacgatcccg agcgcacgct cgccgagttc gaccgcgtgc tcaagccggg
    10021 gggtcggttc gcgttcctcg agcacgtgcg cgccgaggac ggcacgagcc tcgcgggctg
    10081 gcaggaccgc ctgcacggcg cctggctccg cttcggccag ggctgccact gcaaccgccg
    10141 cagcgagcag gcgatcgcgg cctcaccgct gagcctcgag cgggtcgagc gcggcatgct
    10201 cccgaaggag ccgccgatcg tgaagcccta cgtggtcggg accgctttct cggagccgcg
    10261 ctcgatcggg tagcccgcgc gctcccaggt gccgacgccg ccgttcacca cgtgccacac
    10321 gtcggtgagc ccgtagcgct tcagcaggct cgccgccacc gccgagcgct ggcctgacga
    10381 gcacatcgca gccaccggcc gcgacgggtc gaggccctcc ggcacggaat ggatgtcgtg
    10441 gtagggcacg aagaccgaat ccgggatgtg cttctcgcgc cactcggatg cctcgcgcac
    10501 gtcaaggatc tgcacgtcct cacccgactc catccgggcg cgcagctcgt caacgcccac
    10561 tcggtccacc gactcgacct cgccgtgctc catccgccag ctcgtgaagc cgccggcgag
    10621 gaacccgccg agcttgcgga tgccgaccgc cacggagagc cgagcggcgc ggcggccgtc
    10681 ctcgtcgtcg cgcccgacga acacgatctc ctggtcgcga tctgcgatcc aggcgagctt
    10741 ggtgccgaag ccggcgcgca gcatcgtgtt gcacaccgaa cgcggcacgt gcgcgtcgtc
    10801 gaactgcagg tcggtgcgca cgtcaacgat gagcgcaccg tcctgacgct tcatgtccac
    10861 ctgccgcggc gtcagcggcc cgacctccac acccgcggtg acgagggggc cgcggttcag
    10921 gtccacgatc gcctggaagt tcggcggctg cggcccgagc gaggccaccg agcgacgcac
    10981 gaactcgtcc tcctcgggaa cacgcagcat gtcgttgtgg cggcgctcgt agccgatcgt
    11041 cgacgcgatc ttcaggtcca tcccgggacc gccgcacagc gagccgccca ggtggcccgg
    11101 ccacacctcg gtctcgtcgg cgagcgcgag cagcttctcg tggagcgagt ggaagatgcc
    11161 gtgcgcgccc tcctccttgt cgaccgcgag gtcgggccgc gcgatgtcgc ccacgaacag
    11221 cgtgtcgccg gtcagcacgg cccagggctc gtcgccacgg gcggtgtcaa ccagggcgaa
    11281 cgcggtgtgc tccgggcggt gccccggcgt gtgcagggcg cgcacccgca gcgccccgag
    11341 ctcgagctcg tcgccgtcgt cgaagggcac gtggtcgtag gcgacgccgg cctcgcggtg
    11401 cacgtggatc tccgctccgg tcgcggcggc gaggcgccca tgcccggaca cgtggtcggc
    11461 gtggttgtgg gtctcgaaca cgtggtcgat gtgcacaccc atgtagcgcg ccagctcgag
    11521 gtactcgttg atctcgaagc gcggatccac gaccgccgcc acgcccgcgt cctcgtcccc
    11581 gatgaagtag gaggcacagc cgaggtcgtc gtgcgtgatc tgtcggaaga tcatcgtcca
    11641 cacgagccta accggcgtag agtccacata tgaagctcga gcagccgcgc gcgggggtgt
    11701 tccaggcgac gatgacggcc cacgagctgt cggtcctgct cgccggcgcg cgcatgtcgc
    11761 tgaacgtgat gcaggcgggg gaggacgtcg cgaccgcgga ggcgagccag gccctcgaga
    11821 ccgtgctcgc cgacttcgac accgcgatca agcgcgcgac ggctgagcgg gcggacggct
    11881 agcccgcccg ctcgtcgtgc gggtcaggcg tgcgccggct cctgcgcggc catctgctcg
    11941 cgcacgtact cgacactgcg atcgaactct tccttgaact cgtcgtagtc gaactgcatg
    12001 gcgttgaggt acgtgctcca gcccatggtg ttcttcgcga tgaggcaggc ctcgttgacc
    12061 tcctcgtcgg tggcgccgaa cagcttcgct gcttcggtgt ggaagtacgc gcagtagcgg
    12121 cagcgtgtgg cacccgatac cgcgagcccg atgagctcct tgtacttgtt cggaatcgcc
    12181 gtctcggcga gctcgagcgc cttgaagctc tgccactcgg acgagatcag gtagtcgggg
    12241 acggtcttga ggaactccgg tacgagaccg aaggtttcct ggatgtcgcg atcgacctct
    12301 gctcgggtca tgcgtcctcc atcggaaacg ggtggtgttg aggagtggcg gcggagcggt
    12361 gctgtcgccg ccgcgggagt gaaagtgcgg gcctcctttc acgtcgcgtg agcatccgtg
    12421 ccgggtgctg caccgcgggt ccttacggcc caggctaccc ccggccgagc cggcggtaaa
    12481 gacccgcacg atcatgctta accgcggtaa ctctcgccct ggctcaaacg gccgaggccg
    12541 acccaggcga agtccatcac gcgcgagacg acctcctcgc gcggcacgtc ctggtgcacg
    12601 taccaccagt tcgcgagcga ctgcacggag cccgagagct gctgggcgag catctcgatg
    12661 ttgaagctgg cctcgggcgc ggcgctggca ccggggtcgc gcgcgatcag gtccgcgatc
    12721 gcggcggtcg ctcgcagctg gacgcgctcg aacgtctcgg cgaggtccgg atcggcggag
    12781 tcgcggaaca gcatccgcca cgcctcacgg cgctcctcca cgaagccgag gaacgcctcg
    12841 acgccggcgc gcaggcgcat gtcgccggcg tcgccggtgg ctgcactcgc gtcgagccgc
    12901 ccgagcagct cgtcgacctg gtgctcgagc agcgagttgt ggaggtcctt cttggacggg
    12961 aagtgctcgt agatgagcgc cttcgagatg cccgccgcct tggctatctc gtcgatcgaa
    13021 gatccgtgga agccccggcg cgcgaacacc tccatcgcgg cgtcgaggat cgccgcgcgc
    13081 cgctcagcgc cggtgagccg tccgcgtgcc tcggtgtggt ttgcgatggc tcgagtgggg
    13141 gaggtgtcag tcatttactg gtgccgtacc caagacggca tgccgggacg cgtaacggga
    13201 gcctagtcac cgctcgggcg gcgaacccgg tagagatcga cgccgccacg cacgcccttc
    13261 acccggcgcc gaccgacgtg cgtccagtgg tagtcgccct cggcatcctc cttcacctcc
    13321 gcggtggtga gcgcgctgcc cggacgcgcg atcgcggtca cgcggctcgc gaggttcacc
    13381 gggcgtccgt agaggtcgcc cgcgcgcgcg agcgcgggcc cacacgccac gcccacgcgc
    13441 agctgcggga agccgtcgcc ctccgcctcg gccgcgccta gcagggcgag cgcggcccca
    13501 agcagcgcgt ccgaatcggg cgaggcgagc atcgccgcgt cgccgatcat cttcactagt
    13561 cgcaccggcg gctcggtcac ctcgagcgtc atgtcgttca ggcggtccgc aagtgagccc
    13621 acgtcctcgg gcggcagctc ctcgccgagg cgagtgaagc ccaccagatc ggcgaaggcc
    13681 acgctcacgt cgcgcgcgcc ggcgggtcga ccggccgcga tctcggctcc cgtgaccgct
    13741 ccctgccgag caacctcgag ctgctgccgg cgcagcacgt actccatcgt ctggccgaac
    13801 accgggccga gctctcgctg cgcggccgcc agcccgagcg ccacctcgcg ctcgctcgca
    13861 cccgactcga gcagcgtcga agttgtcacg tcgccgagcg cgcgggcgat ctggccgagc
    13921 gactggccca tcacgcggtt cacgtcgagg atcgtctgct ccggaaagcc ggcgtcgagg
    13981 aagcgcttca cgcgccgcgc gccttcgagg tcgaactcgc cgtagatcgc ctcgtcagga
    14041 tcggcgacgg ccaacccgag cgaccgcccg agacgctccc agaactcgag gtcgaggccc
    14101 gcgcgctctg ccacctggcg gccggtgagg cgctcgccgg agccgagcag ctcgcgctct
    14161 accggcagga gcacgagccg atcttcctcg actgcctgct tgagctcgtc gagcgggacg
    14221 ccctcatcga gcagcgtcgt cagcagctca cggcgcgact cgcgcgcatc gccctccacg
    14281 ccttcaagga ggccttccga ctcgaggtcc actcgctgct gggtcacggt gcggcgcagt
    14341 ttcccgtatc ccgcatccgg gaggaggcgg tcaattgtac gctagtgttt ccaactatgg
    14401 agggcgctcg agttcccacc gcagacactc cggatcggct tccgcgcggg ccgcaccggc
    14461 tggcgcgcga ggtagtgctc gcctcccagc gaggccgcat gctcgacgcg atggcgcagt
    14521 cggtggccga gaagggctac gcggcaacca cggtagcgga cgtggtcggc cgcgcgggcg
    14581 tctcgcgcaa gaccttctac gagcacttcc gtgacaagga ggactgcttc ctcgaggcct
    14641 gggacgccgg cgtggagatc ctcctcgacg ccgtggcgca ggcgacgacc gccacggaag
    14701 actggcgcga aggcgtacgc gcgggcacgc gcgcgtacct cgagacgctc gcct
//
LOCUS       DASVXS010000233         7558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1409140, whole genome shotgun sequence.
ACCESSION   DASVXS010000233 DASVXS010000000
VERSION     DASVXS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409140"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="hflX"
                     /locus_tag="VF752_13145"
     CDS             complement(<1..1051)
                     /gene="hflX"
                     /locus_tag="VF752_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX8856535.1"
                     /translation="MEKGRNGRTTETRNGLAYGEGIRNPRARQRALTIAVAPQEPDLA
                     ELRELLRTAGVAVVGEMTQHRVQPDPDRYFGKGKLGELKQAVKESGANLVAVDDELAP
                     RQERNLEEAIGVPVIDRTAIILDIFADHAASAEGKLQVELAQLEYNLARMRGLWTHLE
                     RLGGGIGTRGPGESQIETDRRLARDRISTLKRRLEHTKSTRGVMRRERERAALPTVAL
                     AGYTNAGKSTLLNALTGASAGVRERLFHTLDPTTRSFEIEGRTYLLTDTVGFIRKLPH
                     QLVEAFSATLEEALHADLILHVADASAPEDELAEMLSAVEDVLEEIGAADQPRLLVLN
                     KVDLLEAERRRELGFR"
     gene            complement(1085..1993)
                     /locus_tag="VF752_13150"
     CDS             complement(1085..1993)
                     /locus_tag="VF752_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856536.1"
                     /translation="MSADMPHVEGVTHNWTEVNGFRMHYAEAGPADAEPIVMLHGWPQ
                     HWYEWRHQIPRLAGTNRVICPDLRGLGWSEAPASGYDKSQLADDVLMLLATLGIPRFK
                     LVGHDWGGWVGFLMCLKAPQRIDKYVALNIVHPFQKPDVNFLTYLPRTWYQWVIEVPG
                     GDAILRNTNFVEWAMRLGFADKSAISDADIDVFASRVKTRDGARASQQYYRTFTLKEM
                     PQQIRQRPFDRERLKVPTRLVFGTKDIFIPTSLLRGYEDHADDMLVEYVPDAGHFIAD
                     EKPDLVTDRIESFFGLRKPTQVESAT"
     gene            2046..3020
                     /locus_tag="VF752_13155"
     CDS             2046..3020
                     /locus_tag="VF752_13155"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8856537.1"
                     /translation="MKAFVTGGTGFIGEQLVRKLRERGDEVVALVRSPEKAGKLRDLG
                     AAIVEGDVTDAAAVRRGLEGADAAFHVAAIYKVGIPKSERQQLFDANVHGTEVVLDQA
                     IEAGTPRIVYVSTLNVYGNTRGKVVEEGYERPGDDFLSYYDETKWRAHQVAKDRIANG
                     APIVIVQPGGVYGPNDHSELGNMIDQTRSGKLLFKLFPDAGFNFVYVDDVADGMLLAH
                     DRGRIGESYNLAGPKHTMGDLVQKTAELSGRKPPRLTMPPILMKSAIPIGPVVGKVMG
                     FPPNLGELIKTSDGVTFWGSDQKARSELGWQPRDLDTGLRQTLGQAPI"
     gene            3083..3457
                     /locus_tag="VF752_13160"
     CDS             3083..3457
                     /locus_tag="VF752_13160"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX8856538.1"
                     /translation="MDFQRAACAVGSPPSDEGVVSGEGRRGAEAKAKPGRRDELRKLW
                     DAHLRPRAERNPAHELYLYCYDDAEPNTVRLIEVYADRDSLQANAGADWFADYIRESA
                     PLLDGAPEFATAIPVWAKGFEL"
     gene            complement(3539..4168)
                     /locus_tag="VF752_13165"
     CDS             complement(3539..4168)
                     /locus_tag="VF752_13165"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8856539.1"
                     /translation="MFPSSSTSFLHGQGYGAFQLPNSLLLAASFGTAALFLVEDIEGG
                     YFDKLRAAPVPRSAIVFGRLIAEWAKGIIITTAILLLGLIFGISIASGVFGFVLIVAL
                     TSLWCVVFVGFMQLVALKTRSAAATNSAGLVFFPLLFLTPNFVPRDLLTRPMEIAATA
                     NPVTYIMEALRSLILRDLDWSHILPGFGVVALLGVIMLALNVRVIQNYD"
     gene            complement(4291..5175)
                     /locus_tag="VF752_13170"
     CDS             complement(4291..5175)
                     /locus_tag="VF752_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8856540.1"
                     /translation="MPVDDPEEIYVAEVAVGEERAPMTSPNGSAAAVQAEALVKHFSD
                     RSGVVEAVRGVDLRIEAGEVFGFLGPNGAGKSTTVRMLTTLLTITSGTARVAGVDVAT
                     DPDAVRRRIGVALQEAGLDPRQTGRELLTLQGRLFGLSPEEARARTSELLALVELEDA
                     ADRLIKGYSGGMKRRLDLASALVHQPDVLFLDEPTTGLDPASRLTVWEEVRRINERGT
                     TVFLTTQYLEEADQLCDRLAIIDDGRIVREGTPERLKEELRVRLGLTSQPTLDDVFLD
                     ATGRTRDRVAGEVQEVSA"
     gene            complement(5251..5943)
                     /locus_tag="VF752_13175"
     CDS             complement(5251..5943)
                     /locus_tag="VF752_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856541.1"
                     /translation="MLYLGIAPGPGAFQLAALEEVRTPEPPIRLRATFFEPGSAAQVA
                     AAIAGLGPEVAIGVGAPAGEGRACDAELAERGIPQVPVFAAARELYERLVPLGLYTPA
                     DSAGLREGQVEEGAFHTARVFETSVEGVFAALQGRRLPARRHPLGVARRIDELVEDHI
                     EDDGGELWHRRIEEIEAAACALAAHRYALAHANWLGDPPEGVIVLPGARTPEHFSGEG
                     VIPPVPRRPLPG"
     gene            complement(5951..7117)
                     /locus_tag="VF752_13180"
     CDS             complement(5951..7117)
                     /locus_tag="VF752_13180"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX8856542.1"
                     /translation="MRHSQRLDRLPPYLFAELERKIAAKKAQGVDVISLGIGDPDAPT
                     PDAVVEALAKAARDPGTHQYPSNRGRAEFREAFASFYKSRFGVELDPEGEIIPALGAK
                     ECVFNLNLAFLDADSVALAADPGYPVYTGGPVLSGSEPVLMPLVPELGFIPDLDEIAL
                     GDALRARLMFLNYPNNPTGAVVPDGFFERVVEFAREHDILVVHDASYTETTFDGYVAP
                     SFLETPGAKEVGVEVFSLSKGWNMTGWRTAAIVGNAQAVESYWRLKTNIDSGMFEAVQ
                     LAAVAALTDAADVPKKMSEVYARRRDLVCEALHAIGVDVTPPKGTIYIWAPVPGGHES
                     AAAYCELVLEESGVVVSPGGAYGPNGEGFFRISLTVPDERLTEAVERLRSSLGG"
     gene            complement(7114..>7558)
                     /locus_tag="VF752_13185"
     CDS             complement(7114..>7558)
                     /locus_tag="VF752_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856543.1"
                     /translation="PDDGTGTLNVERREFVFQHVSIGNPQCAIEVREGLEELDLQLYG
                     PPIERHELFPQRTNVSFWRRDGEGEIRARIFERGVGETLSSGTGATGAAVAAVLRGVE
                     SPVTVHLDGGDLEVEVGEDLHVDLTGWAIPVYAGELTDDFLKEIG"
BASE COUNT         1195 a         2630 c         2578 g         1155 t
ORIGIN      
        1 agcggaagcc gagctcgcgg cgacgctctg cctcgagcag gtcgaccttg ttgagcacga
       61 gcagcctggg ctgatcggcg gcgccgatct cctcgagcac gtcctcgaca gccgacagca
      121 tctcggccag ctcatcttcg ggagccgagg cgtccgcgac gtgaaggatc aggtcagcgt
      181 gcagcgcttc ctcgagcgtg gccgagaagg cctcgaccag ctggtgcggc agcttgcgga
      241 tgaagccgac cgtgtcggtg agcaggtacg tgcggccctc gatctcgaac gagcgcgtgg
      301 tggggtcgag cgtgtggaag agccgctcgc gcacgccggc ggaggcgccc gtgagcgcgt
      361 tcaggagcgt ggacttgccc gcattcgtgt agccggccag cgccaccgtt ggaagcgcgg
      421 cccgctcgcg ctcacgccgc atgacgccgc gcgttgactt cgtgtgctcg aggcggcgct
      481 tcagcgtgct gatccggtcg cgcgcgagac ggcggtcggt ctcgatctgc gactcgcccg
      541 ggcccctggt gccgatcccg ccgccgaggc gctcgaggtg ggtccacagg ccgcgcatgc
      601 gcgcgaggtt gtactcgagc tgcgcgagct cgacctgcag cttgccctcg gccgaggcgg
      661 cgtggtcggc gaagatgtcg aggatgatgg cggtccggtc gatcaccgga acgccgatcg
      721 cctcctccaa attgcgctcc tggcgcggcg cgagctcgtc gtcgacagcc accaggttcg
      781 cgccggactc cttcacggcc tgcttcagct cgccgagctt gcccttgccg aaatagcggt
      841 cgggatccgg ctgcacacgg tgctgtgtca tctcgccgac aacggcaacg ccagcggtcc
      901 tgagcagctc gcgcagctcc gcgaggtcgg gctcctgcgg cgccacggcg atcgtgaggg
      961 cacgctgacg cgcccgcggg ttgcggatcc cctctccgta tgccaacccg ttgcgggtct
     1021 cggtcgtgcg cccgttcctg cctttttcca ctacttccag tctagggatg ggagcggcgc
     1081 gcgctcaggt tgcagactcc acctgggtcg gcttgcggag gccgaagaac gactcgatgc
     1141 gatcggtcac gaggtccggc ttttcgtcgg cgatgaagtg gccggcatcc ggcacgtact
     1201 cgacgagcat gtcgtcggcg tggtcctcgt agccgcggag caggctggtc gggatgaaga
     1261 tgtccttcgt tccgaacaca agacgggtcg gcactttcag ccgttcgcgg tcgaacggtc
     1321 gctgacggat ctgctgcggc atctccttca gggtgaaggt gcggtagtac tgctggctcg
     1381 cgcgggcgcc gtcgcgcgtc ttgacccgcg aggcgaagac gtcgatgtcc gcgtcgctga
     1441 tggcggactt gtccgcgaag ccgagccgca tcgcccattc cacgaagttc gtgttgcgca
     1501 ggatcgcgtc gccgcccggt acctcgatca cccactggta ccaggtgcgc ggcaagtacg
     1561 tgaggaaatt gacgtctggt ttctggaacg gatggacgat gttgagcgcg acgtacttgt
     1621 caatgcgctg gggcgctttc aggcacatga ggaagccgac ccaccctccc caatcgtggc
     1681 ctacgagctt gaagcgcggg atgccgagcg tggcgagcag catcagcacg tcgtcggcaa
     1741 gctggctctt gtcgtatccg ctcgcgggcg cctcgctcca gccgaggccg cgcaggtcgg
     1801 ggcagatgac acggttcgta cctgccagcc gcggtatctg gtggcgccac tcgtaccagt
     1861 gctgcggcca gccgtggagc atcacgatcg gctcggcgtc tgccggccca gcctccgcgt
     1921 agtgcatgcg gaagccgttc acctcggtcc agttgtgggt cacaccttcg acgtgcggca
     1981 tgtcggcgct catgagcgcg agcctaacca cccaccaggg ccgcggtcca ggtaggtttc
     2041 gcgccatgaa agcgttcgtg acggggggca cgggcttcat cggcgagcag ctcgtgcgca
     2101 agctccgcga gaggggcgac gaggtggtcg cgctggtgcg ctcgccggag aaggcaggga
     2161 agctgaggga cctcggcgcg gcgatcgtcg agggcgacgt aaccgacgcc gccgccgtca
     2221 ggcgcgggct cgagggagcc gacgcggcgt tccatgtggc ggcgatctac aaggtcggca
     2281 tcccgaagtc cgaacggcag cagctcttcg acgcgaacgt gcacggcacc gaggtcgtgc
     2341 tcgaccaggc gatcgaggcc ggcacgccgc ggatcgtcta cgtctcgacc ctgaacgtgt
     2401 atggcaacac gcgcggcaag gtggtcgagg agggctacga gcgacccggc gacgacttcc
     2461 tctcctacta cgacgagacc aagtggcgcg cgcaccaggt cgcgaaggac cggatcgcca
     2521 acggtgctcc gatcgtgatc gtgcagcccg gcggcgtgta cgggccgaac gaccactcgg
     2581 agctcggcaa catgatcgac cagacgcgca gcggcaagct gctgttcaag ctgttcccgg
     2641 acgccggctt caacttcgtc tacgtggacg acgtcgcgga cggcatgctg ctcgcccacg
     2701 acaggggccg gatcggcgag agctacaacc tcgcgggccc gaagcacacg atgggcgacc
     2761 tcgtgcagaa gacagcggag ctgtccgggc gcaagccgcc gcgcctgacc atgccgccga
     2821 tcctcatgaa gtccgcgatc ccgatcggcc cggttgtggg caaggtgatg ggcttcccac
     2881 cgaacctcgg cgagctgatc aagacctccg acggcgtcac gttctggggc agcgaccaga
     2941 aggcgcgcag cgagctgggc tggcagccgc gcgacctcga cacggggctc cgccagacac
     3001 tgggacaggc gcccatctga gccgcgacat cgcgggagcg ggagaaacgt ccatcccgtt
     3061 tcggacgcag atccatcctt cgatggattt tcagcgcgcc gcttgcgctg taggttcgcc
     3121 gccgtccgac gagggggtgg tgagtggcga aggtcgccgt ggtgctgagg caaaggcgaa
     3181 gccggggagg cgagacgagc tgcgcaaact ctgggacgcg cacctgcgcc cgcgcgccga
     3241 gcggaatccc gcacacgagt tgtatctcta ctgctacgac gacgccgagc ccaacaccgt
     3301 tcggctgatc gaggtgtacg cggaccgcga ctcgctccag gcgaatgcgg gtgccgactg
     3361 gttcgccgac tacatccgcg aatcggcgcc gctgctcgac ggcgcacccg agttcgcgac
     3421 cgcaatccca gtgtgggcaa agggctttga gctctgagcc ccactcacgc ggagtgccag
     3481 cgcgccgcga cctcggctcg ttcgcgggct ggttgcggca ggcgcgcgag ctgaccgttc
     3541 agtcgtagtt ctggatcacc ctgacgttca gcgccagcat gatcacgccg agcaacgcga
     3601 cgacgccgaa gcccggcagg atgtgcgacc agtcgaggtc ccgcaggatc agagaccgga
     3661 gcgcctccat gatgtaggtc accgggttgg cggtggcggc gatctccatt ggcctcgtca
     3721 gcaggtcgcg aggcacgaaa ttcggcgtca ggaacagcag cgggaagaac acgaggcccg
     3781 ccgagttcgt ggccgccgcg ctgcgcgtct tcagcgccac cagctgcatg aagccgacga
     3841 acaccacgca ccagagcgac gtgagggcga cgatgaggac gaacccgaac acaccgctcg
     3901 cgatcgagat gccgaagata aggccgagca gcaggatcgc cgtggtaatg atgatcccct
     3961 tcgcccactc ggcgatgagg cgcccgaaca cgatcgccga ccgcggcacc ggcgccgccc
     4021 gcagcttgtc gaagtagccg ccctcgatgt cctcgacgag gaacagcgcc gccgttccga
     4081 acgaggccgc gaggagcagc gagttcggca gctggaaggc gccgtagccc tgcccgtgca
     4141 ggaacgacgt cgatgaggac gggaagatct tcgcggccta gccgacgttg accaccagga
     4201 agaagagcgg gatgaacagc gtgggcagga gcgcctcagg cgtccgccac ccttcgcgca
     4261 gcgcgcgctg gcccagcacc caggcctgat tcatgccgac acctcctgca cctcaccggc
     4321 gacccggtcg cgcgtgcgcc cggtcgcgtc caggaagaca tcgtcgagcg tcggctggct
     4381 cgtgagcccc aggcgcacgc gcaattcctc cttgagacgc tcgggcgtgc cctcgcgcac
     4441 gatgcgcccg tcgtcgatga tcgccaaccg atcgcacagc tggtccgctt cctcgaggta
     4501 ctgggtggtg aggaatacgg tcgttccgcg ctcgttgatc cggcgaacct cctcccagac
     4561 cgtcagccgg ctcgcgggat cgagcccggt cgtcggctcg tcgaggaaca gcacgtccgg
     4621 ctggtgcacc agcgccgagg cgagatcgag ccggcgcttc atcccgcccg agtagccctt
     4681 gatgagcctg tcggccgcat cctcgagctc gacgagggcg agtagctcgc tggtccgcgc
     4741 tctcgcctcc tccggggaca ggccgaacag ccgtccctgg agtgtgagca gctcgcgccc
     4801 ggtctggcgc ggatcgagcc cggcttcctg gagcgccacg ccgatgcgcc ggcggaccgc
     4861 gtcgggatcg gtggcgacgt ccacgccggc cactctggcg gtgcctgacg tgatcgtgag
     4921 cagcgtcgtg agcatccgga cggtggtcga cttgccggcc ccgttcgggc ccaggaagcc
     4981 gaagacctca cccgcctcga tcctcaggtc gacgcctcgg acggcctcca ccactccgga
     5041 cctgtctgag aagtgcttca ccaacgcctc cgcctggacg gcagccgcgc tgccgttcgg
     5101 gctggtcatg ggcgctcgtt cctcgcccac cgctacttcc gccacgtaga tctcctcggg
     5161 gtcgtctacg ggcataagac cagagccggc cgcgaaacta atcgaccgcc cccaccctgg
     5221 cttctccaaa aatgctcggt ccggcggagg ctagcccggc agcggccgcc gcgggacggg
     5281 cgggatcacg ccctcgccgc tgaagtgctc cggcgtgcga gcgccgggga gcacgatcac
     5341 gccttccggc gggtcgccga gccagttcgc gtgtgcgagc gcatagcggt gcgccgcgag
     5401 tgcgcacgca gccgcctcga tctcttcgat gcggcggtgc cagagctcgc cgccgtcgtc
     5461 ctcgatgtga tcctcgacca gctcgtcgat ccgccgcgcc acgcccagcg gatgccgccg
     5521 cgccggcagc cggcgccctt ggagcgccgc gaacacgccc tccacgctcg tctcgaacac
     5581 gcgcgccgtg tggaatgcgc cctcctccac ctgtccctcg cgcaggccgg cgctatccgc
     5641 aggcgtgtag aggcctagcg gcacaagccg ctcgtacagc tcccgcgcgg ccgcaaacac
     5701 gggcacctga gggatgccgc gctccgccag ctcggcgtcg caggcgcggc cctcgccggc
     5761 cggcgcgccc accccgatcg cgacctctgg cccgagcccg gcgatcgccg ccgccacctg
     5821 tgccgcggag cccggctcga agaaggtggc ccggagccga atcggcggct cgggcgtgcg
     5881 gacctcctcg agcgccgcca gctggaacgc gccggggccc ggggcgattc cgaggtagag
     5941 catgggtgcg ctagcccccc aggctcgagc gcagccgctc gacagcctca gtgagccgct
     6001 cgtcgggcac ggtcagagag atccggaaga agccctcgcc gttcggcccg tacgcgccgc
     6061 ccggcgacac caccacgccg gactcctcga ggacgagctc gcagtaggcg gccgccgact
     6121 cgtggccccc cggcaccggc gcccagatgt agatcgttcc cttgggcggc gttacatcca
     6181 caccgatcgc gtgcagcgct tcgcacacca ggtcgcgccg gcgcgcgtaa acctcgctca
     6241 ttttcttcgg cacatccgct gcgtcggtca gcgccgcaac cgccgcgagc tgcacggcct
     6301 cgaacatgcc ggagtcgatg ttcgtcttca gccgccagta ggactcgacg gcctgagcgt
     6361 tcccgacaat cgccgcagtg cgccagccgg tcatgttcca gcccttcgag agcgagaaca
     6421 cctcgacgcc cacttccttc gcgcccggcg tctcgaggaa gctcggcgcc acgtaaccgt
     6481 cgaaggtcgt ctcggtgtag gaagcgtcgt gcaccaccag gatgtcgtgc tcgcgcgcga
     6541 actccaccac ccgctcgaag aagccgtccg gcacgaccgc gcccgtcggg ttgttggggt
     6601 agttgaggaa catcagccgc gcgcgcagag cgtccccgag cgcgatctcg tcgaggtcgg
     6661 ggatgaagcc gagctcgggc acgagcggca tcagcaccgg ctcggagccg gagagcaccg
     6721 ggccgcccgt atagaccgga tagcccggat ctgcggcgag cgccacgctg tcggcgtcga
     6781 ggaacgcgag gttgaggttg aacacgcact ccttcgcgcc gagcgccggg atgatctcgc
     6841 cctcgggatc gagctcgacc ccgaagcggc tcttgtagaa gctcgcaaac gcctcgcgga
     6901 actcggcgcg gccgcggttc gacgggtact ggtgcgtgcc ggggtcccgg gccgccttcg
     6961 cgagcgcctc caccaccgcg tcgggagtcg gtgcgtcggg gtcgcctatc ccgaggctga
     7021 tcacgtccac gccctgcgcc ttcttcgccg cgatctttcg ctcgagctcc gcgaacaggt
     7081 acggcggcag gcggtcgagc cgctggctgt gcctcatcca atctccttca ggaagtcgtc
     7141 ggtcagctcg ccggcgtaga cggggatcgc ccagccggtg agatccacgt gcaggtcctc
     7201 gcccacctcc acctcgaggt cgccgccgtc gaggtgcacg gtcacggggc tctcgacgcc
     7261 gcgcaacacc gcggccactg cggcgccggt ggctcctgtg cccgaggaca gcgtctcacc
     7321 aacgccgcgc tcgaagatgc gcgcgcggat ctcgccctcg ccgtcgcgcc gccagaacga
     7381 cacgttcgtg cgctgcggga agagctcgtg acgctcgatt ggcggcccgt agagttggag
     7441 gtccagctcc tcgaggccct cgcgcacctc gatcgcgcac tgcgggttgc cgatcgagac
     7501 gtgctggaag acgaattcgc gcctctcgac gttgagagtc ccggtgccgt cgtcaggg
//
LOCUS       DASVXS010000234         8564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_881801, whole genome shotgun sequence.
ACCESSION   DASVXS010000234 DASVXS010000000
VERSION     DASVXS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881801"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF752_13190"
     CDS             complement(<1..498)
                     /locus_tag="VF752_13190"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX8856544.1"
                     /translation="MAVRISSGISSADAGVDSFAEAAARAALGLGGLPADIAIVFAAA
                     PNLSHADLGVEIVSERLGSPAAIAGCGAQGVLAGGRELETGGVAVWAASMPDAELETF
                     HLDAVPAGEDQVAVAGMPELDDAEAAILIADPYTFPVEPLLTRLGDDLPGLPVVGGLA
                     SGGGAP"
     gene            533..1033
                     /locus_tag="VF752_13195"
     CDS             533..1033
                     /locus_tag="VF752_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8856545.1"
                     /translation="MTNRRQLDPDRLGDHIDRLYRAAWGLCGSKEAAEDLVQDTFAKV
                     LARPRFLRNDDDLGYLLRVLRNTFISQLRSRKPSSVPLDEGLDVPEGVTTGQPDEAAE
                     LRLVYPAIADLPQEFREAVVAVDVVGLSYSEAAKALKVPEGTLTSRVFRGRRQLAQAL
                     EPERNQ"
     gene            complement(1027..1806)
                     /locus_tag="VF752_13200"
     CDS             complement(1027..1806)
                     /locus_tag="VF752_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856546.1"
                     /translation="MATERSHQIDEAELARLADGTLPEERRAIVERAVAESPALQAQL
                     AEQRQALDAVHAAVADVEAPPSLRARIEAERRGRRAAAGSRRRYVLVAAAAAAVVLAV
                     LVALPSGSPGGPSVVQAAAIGLQPPTAAAPARYDHSNVLLNRSESGVPFPNWAQAFGW
                     TPVGVRTDEVKGRGTTTVYYEHGGKRLSYTIVSGDALDIPQGVWRGEQNGVKLWSLQS
                     GPRTVLVWERKGHTCVMSSIGVSRAELATLAAWKARGELPY"
     gene            complement(1850..2770)
                     /locus_tag="VF752_13205"
     CDS             complement(1850..2770)
                     /locus_tag="VF752_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8856547.1"
                     /translation="MAGHILVGTSSWADPGFVEDWYPQGMPPRERLPWYAERFEFVEV
                     NSSFYGIPDRSTVERWVEATPDSFTFDYKLHRLLSRHAAPENSLPKDMQSEVDVNQRG
                     RVQLTSELEAEVIDRTLESVEPLVDAGKLSAFLLQLTPAFAPRKAELEELAPLVERFR
                     PHPVAIEFRHRGWVEGNQAERTFAFLSEIGAVYVCVDAPPEKHVPIMPPIDAVTNEEL
                     AYMRLHGRNTQGYLRGKTVAERFGWVYTDDELHEVEGRVKRLAEDAGAVHVAFNNNRS
                     DDAPTAAQRFRTLLGQEPGPPPEGEQLRIA"
     gene            complement(2804..3814)
                     /locus_tag="VF752_13210"
     CDS             complement(2804..3814)
                     /locus_tag="VF752_13210"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HEX8856548.1"
                     /translation="MKLDSFARYPLMFGPSPVHRLERLTAHLGGASLWAKRDDCNSGL
                     AYGGNKTRKLEYLVADAISKGCDTLVSIGGVQSNHTRQVAAAAARTGLKCVLVQESWV
                     HWPDVVYDRVGNIQLSRIMGADVRLVDAGFGIGFKASWEQALADVEEAGGKPYAIPAG
                     ASDHELGGLGFAGWAQELAGQEKELGVFFDTVVVCSVTGSTQAGMLAGFAAQGTERRV
                     IGIDGSAKPRETHEQVARIARRTAELIEVGRELRDEEIVLDDRYHAGVYGIPDDQTLE
                     AIRTAARLKGMITDPVYEGKSMAGMIDLVSNGEIERDSNVLYAHLGGQPALSAYAGLF
                     RF"
     gene            complement(3831..4451)
                     /gene="pdxH"
                     /locus_tag="VF752_13215"
     CDS             complement(3831..4451)
                     /gene="pdxH"
                     /locus_tag="VF752_13215"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX8856549.1"
                     /translation="MSEALHERPLREEDLDPDPFRQFAAWYAEAGEAVRIPEAMAVAT
                     ATPDGSPSVRMVLLKQHGEDGFVFFTNYESRKGEELAANARAALLFYWDPLGRQVRIE
                     GPVERTTAEETLEYVRTRPRGSRLSALASPQSRPVAGREELEERVASLTEQYSDTDLP
                     VTENWGGFRVMPKLFEFWQNRSDRLHDRFIYRPDGAGGWTIERLAP"
     gene            complement(4448..4849)
                     /locus_tag="VF752_13220"
     CDS             complement(4448..4849)
                     /locus_tag="VF752_13220"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856550.1"
                     /translation="MGDLADTVTDGIRLFNERRYEESLAAFTDDVEWRTGLHPLLGID
                     ATRGRNGVREFWREAHSGWDDFRVDPIVVEELDGSPVLVTARYFGRGHASGVEFENQV
                     FCLYELRDGLICSVRDFGSREAALAAAKEEE"
     gene            complement(4861..8133)
                     /locus_tag="VF752_13225"
     CDS             complement(4861..8133)
                     /locus_tag="VF752_13225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX8856551.1"
                     /translation="MTYVELHSHSAYSFLDGASHPLELAAVAAEQGHASMALTDHDGL
                     HGAMEFAKSAQALGVRPITGAELTLEDGHHLTLLCETTEGYRNLCRLITLAHAHTRDN
                     PSREAGQPTTRLEDLERHTEGLFCLSGCARDGMLAARVERGDHVGAAAAGRRLLAAFG
                     PERFRVELQRPFGRHDRRRNRLLAQLAERLGVPAVATGNVHVHSRARSALQDVFVATR
                     LRMTLDECEPLRRGNRSHALATSEAMAGRFAEHPDAIEESGRIAERLEFDLTRDLGYR
                     YPGSEDGSADRKLAELCAEKVVQRYSGRGLEEARARLDEELRIIRSLKLSGFFILHRD
                     LLELAREVAVEVRGPDTARALLPPGRGRGSSVSSIVCYLTGLSHIDPIENRLLMGRFL
                     NEELVAMPDIDLDFPRDIREVLIPRVHERYGHDRSALVAAFPTYRVRSGVRDFGKALG
                     LAPGEVERVARSSDPFETSGMDGDVASAVGERRSRSPRWQALVRLVHEAHGLPRHMSQ
                     HPGGMVIATEPLQDICPIQPAAMEARQMVQWDKDSCGDAGFLKIDLLGLGMLSAVERC
                     VEEIARVRGERIDLSRIPFDDQRVYAEIQEAETTGVFQIESRAQMQMLKRTRPETLDD
                     LTVQVALVRPGPIIGGAVHPYIERRKALRADPSYQVPYEHSSLAPVLADTLGAIVFQD
                     QVIEVAMAFAGFSQGEAEGLRRAMSRKRSEEAMRAFEQKFIEGAMALHGVDRGCAERV
                     YTQIVGFSGFGFPKAHSAAFGLLAYQSTWLRVHYGPEFLAGLLNEQPMGFYPPDALVH
                     DAQRRSIEVRPPDVLASRAECTVDDDLAVRIGLGYVVGVREAEVREMVEERSRSGQFS
                     SLSDLAGRCAAQRDSLERLAWAGACDSLAPNGRREALWLLGVASPGVNVKGGVQLALP
                     LETGDAPELPELSAWDRMVADYGSTDITLREHPIELMRPAIDSDSVTSVDLERIPSGR
                     RVKVAGLVVARQRPATANGVTFMLLEDEHGTINLIVPPPVYQRCRLAVRAEPIVSAEG
                     KLEKREGVVNIVVDMVRRLDTPDMPQGEVRHIEPVRTWSSEAGELRSVAPKPHSFGRR
                     AG"
     gene            8182..8418
                     /locus_tag="VF752_13230"
     CDS             8182..8418
                     /locus_tag="VF752_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856552.1"
                     /translation="MSERLSLTAVFEPVEDGWIQARIEELPEVITAAPSKEEAKELLL
                     DALREYLLSLAQDTRPVAVSDRATKEALELSLSN"
BASE COUNT         1271 a         3112 c         2903 g         1278 t
ORIGIN      
        1 gggcgcgccg cctccgctcg ccaagccgcc cacgaccggc aagccgggca aatcgtcacc
       61 cagcctggta agcagcggct cgaccgggaa ggtgtaagga tcggcgatca ggatcgcggc
      121 ctcggcgtcg tcgagctcgg gcatgcccgc caccgccacc tgatcctcgc ccgccggaac
      181 ggcgtcgagg tggaacgtct cgagctccgc gtccggcatc gacgccgccc agacggccac
      241 cccaccggtc tcgagctcgc gcccgccggc aagcacgccc tgcgcgccac agcccgcgat
      301 tgcggccggc gagccgagcc gctcgctcac gatctctacc ccgaggtcgg cgtgcgagag
      361 gttgggagcc gccgcgaaca cgatcgcgat gtcggcgggc aggccgccaa gacccagcgc
      421 cgcgcgcgcg gcggcctcgg caaaggaatc gacacccgcg tcggccgatg agatgccaga
      481 cgaaatccgg acggccatat ctctatcttc gcggattggc cggacccgca agatgaccaa
      541 ccgccgccag ctcgacccgg atcgcctggg cgatcacatc gaccggctct accgcgccgc
      601 gtggggcctc tgcgggtcga aggaggcggc cgaggacctc gtgcaagaca cgttcgcgaa
      661 ggtgctcgcc cgtccgcggt tccttcgaaa cgacgacgac ctcggctatc tgctgcgggt
      721 cctgcgcaac accttcatca gccagctgcg gagccgcaag ccgtcctcgg taccgctcga
      781 cgagggcctc gacgtgcccg agggcgtgac caccgggcag cccgacgagg ccgccgagct
      841 tcggctcgtc taccccgcga tcgcggatct gccccaggag ttccgcgagg ccgtcgtggc
      901 ggtcgacgtg gtgggcctct cctactccga ggccgcgaag gcgctgaagg taccggaggg
      961 cacgctcacg agccgcgtct tcaggggccg ccgccagctc gcgcaggcgc tcgagcccga
     1021 gcgcaatcag tagggcagct cgccccgcgc cttccaggcg gcgagcgtcg cgagctctgc
     1081 gcgcgacact ccgatgctcg acatcacgca ggtgtggccc tttcgctccc acacgagcac
     1141 ggtgcgcggc ccagactgga ggctccaaag cttcacgccg ttctgctcgc ccctccacac
     1201 accctggggg atgtcgagcg cgtcacccga aacgatcgtg taagagagac gcttgccgcc
     1261 gtgctcgtag tacacggttg tggttccgcg gcccttcact tcgtcggtgc gcacgccgac
     1321 cggagtccag ccgaacgcct gcgcccagtt cgggaacgga acgccggact cgctgcggtt
     1381 gagcaggacg ttcgagtggt cgtagcgcgc gggtgccgcg gcggtcggcg gctgaaggcc
     1441 gatcgcggcc gcctgaacaa ccgagggccc gcccggcgag ccggacggga gggcgaccag
     1501 gaccgcgagc accacggccg ccgcggccgc tgcgacgagc acgtagcggc gcctcgaacc
     1561 cgctgcggcg cggcggcctc gccgctcagc ctcgatgcgc gctcgcaggg agggcggcgc
     1621 ctcgacatcg gccaccgccg cgtgcacggc atccagcgcc tgccgctgct cagcaagttg
     1681 cgcctgcagg gcgggagact cggcgaccgc tcgctccacg atcgcgcgcc gctcctcggg
     1741 cagcgtgcca tcggcgagcc gtgccagctc ggcctcgtcg atctgatgtg agcgctcggt
     1801 cgccatgctc cctatgaaac aagacgcgct gtcgggcgtg aatcgtccct catgcgatcc
     1861 gaagctgctc gccctcgggc ggcgggccag gctcctggcc gaggagcgtg cggaaacgct
     1921 gcgccgcagt gggcgcgtcg tccgaccggt tgttgttgaa cgccacgtgc acggcgcccg
     1981 cgtcctccgc gagccgcttc acgcgcccct cgacctcgtg cagctcgtcg tcggtgtaca
     2041 cccagccaaa gcgttcggcc accgtcttgc ccctgaggta cccctgtgtg ttgcgcccgt
     2101 gcaagcgcat gtacgcgagc tcctcgttgg tgacggcgtc gatcggcggc atgatcggca
     2161 cgtgcttctc gggcggcgcg tccacgcaca cgtagacggc gccgatctcc gagaggaacg
     2221 cgaacgtgcg ctctgcctgg ttgccctcga cccaaccccg gtgccggaac tcgatcgcga
     2281 ccggatgcgg ccggaagcgc tcgacgagcg gcgcgagctc ctcgagctcc gccttgcgcg
     2341 gggcaaaagc gggcgtgagc tgcagcagga aggcgctcag cttgccggcg tcgacgaggg
     2401 gctcaaccga ctcgagcgtg cggtcgatca cctcagcttc gagctcgctc gtgagctgca
     2461 cgcggccgcg ctggttcaca tccacctcgc tctgcatgtc cttcggcagc gagttctctg
     2521 gcgccgcgtg gcgcgagagc aggcggtgca gcttgtagtc gaaggtgaag ctgtcgggcg
     2581 tagcctccac ccagcgttcg accgtactgc ggtccgggat cccgtagaag ctcgagttca
     2641 cctcgacgaa ctcgaagcgc tccgcgtacc agggcagccg ctccctcggc ggcatgccct
     2701 gcggatacca gtcctcaacg aagccggggt ccgcccagct cgaggtgccg acgaggatgt
     2761 gtccagccat gcgtctgacg ttagcgcggg gcctcgaccg gatttagaag cgaaacaggc
     2821 ccgcgtaggc gctcagcgcc ggctgaccgc cgagatgcgc gtagagcacg ttggagtcgc
     2881 gctcgatctc gccgttcgag acgaggtcga tcatcccggc catcgacttc ccctcgtaga
     2941 cggggtcggt gatcatgccc ttgaggcgcg ccgccgtccg gatcgcctcc agggtctggt
     3001 cgtcggggat gccgtagacg ccggcgtggt agcggtcgtc gagcacgatc tcttcgtcgc
     3061 gcagctcacg gcctacctcg atcagctccg ccgtccggcg tgcgattcga gccacctgct
     3121 cgtgagtctc gcgtggcttc gccgagccgt cgatgccgat cacgcggcgc tcggtgccct
     3181 gtgccgcgaa ccccgcgagc atccccgcct gcgtgctgcc cgtgaccgag cacacgacga
     3241 ccgtgtcgaa gaaaacgcct agctccttct cctgcccggc cagctcttgc gcccagccgg
     3301 cgaacccgag cccgcccagc tcgtggtcgg aagccccggc cggaatcgcg tacggcttgc
     3361 cgcctgcctc ctccacatcc gcaagcgcct gctcccagct cgccttgaag ccgatgccga
     3421 atcccgcatc gaccaagcgg acgtcggcgc ccatgatgcg gctgagctga atgttgccga
     3481 cgcggtcgta gaccacgtcg ggccagtgca cccagctctc ctgcaccagc acgcacttca
     3541 gcccggtgcg cgcggccgcc gcggccacct gccgcgtgtg gttcgactgg acgccgccga
     3601 tcgacaccag cgtgtcgcag cccttcgaga tggcgtcggc caccagatac tcgagcttgc
     3661 gcgtcttgtt gccgccgtag gccaggccgc tgttgcagtc gtcgcgcttc gcccagaggc
     3721 tcgcgccgcc caggtgcgcc gtcagccgct cgagccgatg gaccggcgac ggcccgaaca
     3781 tgagcggata gcgggcaaac gagtcgagct tcatgttgat cctcttccgt tcacggagcc
     3841 agccgctcga tcgtccaccc gccggcgccg tcgggccggt agatgaagcg gtcgtgcagg
     3901 cgatcgctgc ggttctgcca gaactcgaac agcttcggca tcacgcggaa cccaccccag
     3961 ttctcggtca cgggcaggtc cgtgtcgctg tactgctcgg tcagcgaagc cacgcgctcc
     4021 tcgagctcct cgcggcctgc gaccggccgg ctctgcggcg atgccagcgc gctcaggcgg
     4081 ctgccgcgcg gccgggtgcg cacgtactcg agcgtctcct cggccgtcgt gcgctcgacg
     4141 ggcccctcga tccgcacctg ccgcccgagc gggtcccagt agaacagcag cgcggcacgc
     4201 gcgttggcgg cgagctcctc gcccttgcgg ctctcgtagt tggtgaagaa cacgaagccg
     4261 tcctcgccgt gctgcttcag gagcaccatg cgaaccgacg ggctgccgtc cggcgtggct
     4321 gtggcgaccg ccatcgcctc gggaatccga accgcctcgc cggcctcggc gtaccaggcc
     4381 gcgaactgcc ggaaggggtc tgggtcgagg tcctcctccc ggagcggacg ttcgtgcaga
     4441 gcctcgctca ctcctcctcc ttcgcggccg caagggccgc ctcgcgcgag ccaaaatcgc
     4501 ggaccgagca gatgagcccg tcgcgcagct cgtagaggca gaacacctgg ttctcgaact
     4561 ccacgccgct cgcatgcccg cggccgaagt agcgggcggt gacgagcacg ggggagccgt
     4621 cgagctcctc gaccacgatg gggtcgaccc ggaaatcgtc ccaaccggag tgggcctcgc
     4681 gccagaactc gcgcacgccg ttgcgtccgc gcgtggcgtc tatcccgagc agcgggtgca
     4741 accccgtgcg ccactccacg tcgtcggtga acgccgccag cgactcttcg tagcggcgct
     4801 cgttgaacag ccggattcca tcggtgacgg tgtcggcaag gtcgcccatc gcgcgaagta
     4861 tcacccggcc cgccgaccga agctatgcgg cttcggcgcc acggagcgca gctcgcccgc
     4921 ttcgctcgac cacgtgcgca ccggctcgat gtggcgaacc tcgccctgcg gcatgtcggg
     4981 cgtgtcgagc cggcgcacca tgtcgaccac gatgttcacc acgccttccc gcttctcgag
     5041 cttgccctcg gcggacacga tcggctccgc ccgcacggcc aggcgacagc gctggtagac
     5101 cggtggcggc acgatcaggt tgatcgtgcc gtgctcgtcc tcgagcagca tgaaggtgac
     5161 cccgttcgcc gtggcagggc gctgccgcgc taccacgagg ccggcgacct tcacccggcg
     5221 cccggacggg atgcgctcga ggtccacgct cgtgaccgag tcggagtcga tcgccgggcg
     5281 catcagctcg atcgggtgct cgcgcagcgt gatgtccgtg gagccgtagt cggcgaccat
     5341 gcggtcccac gcgctcagct cgggaagctc cggcgcatcg cccgtctcga gcggcagcgc
     5401 gagctgcacg ccgcccttca cgttcacgcc aggcgacgcc acgccgagca gccacagcgc
     5461 ctcgcggcgg ccgttcggcg cgagcgagtc gcaggcgccc gcccacgcca ggcgctcgag
     5521 cgagtcgcgt tgcgccgcgc agcgcccggc gaggtcggac agcgagctga actgaccgct
     5581 tcgcgagcgc tcctcgacca tctcgcgcac ctccgcctcg cgcacgccga ccacgtagcc
     5641 gagcccgatc cgcaccgcca ggtcgtcgtc cacggtgcat tccgccctgc tcgcgagcac
     5701 gtcgggcggc cgcacctcga tgctgcgccg ctgcgcgtcg tgcaccagcg cgtcgggcgg
     5761 atagaagccc atcggctgct cgttcagcag cccggcgagg aactcaggcc cgtaatgcac
     5821 gcgcagccac gtggactggt aggcgagcag cccgaacgcg gccgagtgtg ccttcgggaa
     5881 gccgaagccc gagaagccca cgatctgggt gtagacgcgc tcggcgcaac cgcggtcgac
     5941 gccgtggagc gccatcgcgc cctcgatgaa cttctgctcg aaggcgcgca tcgcctcctc
     6001 cgagcgcttg cggctcatcg cgcgccgcaa gccctctgcc tctccctgtg agaagccggc
     6061 gaacgccatc gccacctcga tcacctggtc ttggaacacg atggcgccga gcgtgtcggc
     6121 cagcaccggc gcgagcgacg agtgctcgta cggcacctgg tacgacgggt cggcgcgcag
     6181 tgccttgcgc cgctcgatgt aagggtgcac ggcgccgccg atgatcgggc caggccgcac
     6241 gagcgccacc tgcacggtga ggtcgtcgag cgtctccggc cgcgtgcgct tgagcatctg
     6301 catctgggcg cggctctcga tctggaacac gccggtggtc tccgcctcct ggatctcggc
     6361 gtacacgcgc tggtcgtcga aggggatgcg cgagaggtcg atccgctctc cgcgcacgcg
     6421 cgcgatctcc tccacgcagc gctcgaccgc ggacagcatc cccaggccga gcaggtcgat
     6481 cttgaggaag cccgcgtcgc cgcaggagtc cttgtcccac tgcaccatct ggcgtgcctc
     6541 cattgccgcc ggctggatcg ggcagatgtc ctgcagcggc tcggtcgcga tcaccattcc
     6601 tcccgggtgc tggctcatgt gccgcggcag cccatgcgcc tcgtgcacga gccgcacgag
     6661 tgcctgccag cgcggcgagc gcgatcgccg ctcccccacc gcgctcgcca cgtcgccatc
     6721 catgccgctc gtctcgaagg gatccgacga gcgcgcgact cgttccacct cgccgggcgc
     6781 gaggccgagc gccttgccga agtcgcgcac gcccgagcgc acgcggtagg tggggaaagc
     6841 cgcgacgagc gccgagcgat cgtgcccgta gcgctcgtgc acgcgcggga tcagcacctc
     6901 gcggatgtcg cgcgggaagt cgaggtctat gtcgggcatc gccaccagct cctcgttgag
     6961 gaagcggccc atcaacagcc ggttctcgat cgggtcgatg tgcgagaggc cggtgaggta
     7021 gcagacgatc gacgacacgc tcgatccgcg cccgcgcccg ggcggcagga gcgcccgcgc
     7081 ggtgtccgga ccgcgcacct cgaccgccac ctcgcgcgcg agctcgagca ggtcgcggtg
     7141 gaggatgaag aagccggaca gcttcagcga gcggatgatg cgcagctcct cgtcgagccg
     7201 tgcgcgcgcc tcctcgagcc ctcgccccga gtagcgctgc acgaccttct cggcgcacag
     7261 ctccgcgagc ttgcggtcgg ccgagccgtc ctcagagccg gggtagcgat agccgaggtc
     7321 gcgcgtgagg tcgaactcga gccgctctgc gatccggccc gattcctcga tcgcatccgg
     7381 gtgttcggcg aagcggcccg ccatcgcctc ggaggtcgcg agcgcgtgcg agcggttgcc
     7441 gcgccgcagc ggctcgcatt cgtcgagcgt catgcgcagg cgggtggcca caaacacgtc
     7501 ctgcagggcg gagcgcgcgc gtgagtgcac gtgcacgttg ccagtcgcaa ccgctggaac
     7561 gcccaggcgt tcggcgagct gcgcgagcag ccggttgcgc cggcggtcgt gacgaccgaa
     7621 cgggcgctgc agctccaccc tgaagcgctc cgggccgaag gctgcgagca ggcgtcgccc
     7681 cgctgcggca gcgcccacgt ggtcaccccg ctcgacccgc gcggccagca tgccgtcgcg
     7741 cgcacagccc gaaaggcaga agagtccctc cgtgtggcgc tcgagatcct cgagccgcgt
     7801 ggtgggctga ccggcctcgc ggctcgggtt gtcgcgcgtg tgcgcgtgcg cgagtgtgat
     7861 cagccggcag aggttccggt agccctcggt ggtctcgcac aggagcgtga ggtggtggcc
     7921 gtcctcgagc gtcagctcgg cgccggtgat cggccgcaca ccgagcgcct gcgccgactt
     7981 cgcgaactcc atcgcgccgt gcagcccgtc gtggtcggtg agcgccatag aggcgtggcc
     8041 ctgttcagcc gcgacggctg ccagctcgag cgggtgcgag gcgccgtcga ggaacgagta
     8101 ggcggagtgg ctgtgcagct cgacgtatgt catctgtccc gatcgcaggt ttcgggcctg
     8161 tcgctgggat agccttgagg catgtccgag cggctttctc tcactgccgt cttcgagccg
     8221 gttgaagacg ggtggatcca ggcgcgaatc gaagaactgc ctgaagtgat cacagcggct
     8281 ccctccaagg aagaagccaa ggaattgctg ctggatgctc ttcgcgaata cctgctgtcg
     8341 ctggcgcagg acacgcgacc ggtggcggtg agcgaccggg cgacgaagga agcactcgag
     8401 ctcagtttga gcaactgatt ccgacaagct cttagcagtt ccggggccat cctgacctag
     8461 ccgcgctgca ggaaccagcg gccggtgggc aggtcgcgga acaccacggt gttgcgcccg
     8521 tcttcgagca cgagctcgaa gtagcgccgc cgcagcggcc gctc
//
LOCUS       DASVXS010000235        21993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1094080, whole genome shotgun sequence.
ACCESSION   DASVXS010000235 DASVXS010000000
VERSION     DASVXS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094080"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /gene="rpsB"
                     /locus_tag="VF752_13235"
     CDS             complement(<1..738)
                     /gene="rpsB"
                     /locus_tag="VF752_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX8856553.1"
                     /translation="MAQEVGIRELLEAGVHFGHQTRRWNPKMRRFIFGERGGIHIIDL
                     EQTEQLLKRAQDFAGEVASRGGTILFVGTKKQARDAVKEAATAAGMPYIDQRWLGGLL
                     TNFQTISKRIKRLHDLTDWTENGTLELLPTRERIAAIAEREKLEVNLGGVRNLQRTPD
                     AVFVVDLKTEAIGVREAQRLRIPIIGLVDTNVDPDPVTYPIPGNDDAIRSCKLIVDAI
                     GSVAAERAAIFRAEEERARQEREEQERR"
     gene            complement(926..1870)
                     /locus_tag="VF752_13240"
     CDS             complement(926..1870)
                     /locus_tag="VF752_13240"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8856554.1"
                     /translation="MSARVAFTAGEDDAGRRLDVRLAEEPAIGSRAKAQRLIESGAVT
                     VDGRARQKRHHLAPGEAVEVLIEERPPADSRGGADVPFEVVYEDDDLMVVDKPAGVVV
                     HPAPGHPEGTLAQALAARGAAGGDPLRPGIVHRLDRDTSGLLVVAKSEPVHRALQQML
                     RERKITREYLALVEGRPASRTGMIDAAVGRDRRERTLISTHTDAGRDAVTHFELVEAL
                     PRTSLLRVRLETGRTHQIRAHMAAIGHPVVGDTQYGGAPSGARLGLARQFLHSSTLMF
                     EHPTRGENVRCESKLPAELCRAFDAAKREPASEGPDGG"
     gene            complement(1867..2322)
                     /gene="lspA"
                     /locus_tag="VF752_13245"
     CDS             complement(1867..2322)
                     /gene="lspA"
                     /locus_tag="VF752_13245"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8856555.1"
                     /translation="MSPRLRAWLLALFTTVLVIGIDQATKAAVRSSLALGESRKLFLG
                     IDLSYVRNKGVAFGFFGGGGAIVTVLTSLALVALLLYFVLRTTTPLLWLPTGLVLGGS
                     LGNLWDRARDGAVTDFIDPEFWPAFNFADICIVVGVLGLLYVIERPHRT"
     gene            complement(2319..2615)
                     /locus_tag="VF752_13250"
     CDS             complement(2319..2615)
                     /locus_tag="VF752_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX8856556.1"
                     /translation="MAGSQQYLSERDGSALVWVRVRAGASRNELVGERAGALLVRVTA
                     PPVDGKANDALRKLVAKIVGVPPGRVALVSGASGRDKLLRLEGLSAAAAGERLP"
     gene            complement(2620..2925)
                     /locus_tag="VF752_13255"
     CDS             complement(2620..2925)
                     /locus_tag="VF752_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX8856557.1"
                     /translation="MTLVLAVTRDDIASYVGTLIIVYIVLIFIRILMSWLPRIPYNRW
                     LDMFLTFVREVTDPFLNVFRRILPPIRIGPGAIDLSPTIAIFVLFIIERIVVSAIAG"
     gene            complement(2922..3680)
                     /gene="proC"
                     /locus_tag="VF752_13260"
     CDS             complement(2922..3680)
                     /gene="proC"
                     /locus_tag="VF752_13260"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX8856558.1"
                     /translation="MRIGLIGAGNMASALARGFGEPVLVFDPVAGRAEALAEELGGEA
                     LASNAEVAEQADVVFLCHKPAQLDEVAAEAHDNAKAVVSILAATPVARVEEAYPDRPV
                     YRFLPNIPVEVKQGVACYVPGRLASDGPESDVIGLFERVGEVVRLPSEPLIEPAMALM
                     SSGPAFFALVVEALVEAGVKQGLARDDAAKLTVETMGGSAEVLRRGDLDATALRTRVT
                     SPGGVTARGLAALERGGLRTAFDDAVAAVVEATR"
     gene            complement(3717..4253)
                     /locus_tag="VF752_13265"
     CDS             complement(3717..4253)
                     /locus_tag="VF752_13265"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX8856559.1"
                     /translation="MALRDSWHRALVYFGLAEEDDGYDRAEEELEPEHELEDRYRERP
                     NVRRLQSRRRRDEFDDIFADDEPAGRGGRQTRVLRPVGNGRSDVQVHLVVPKNFNDAQ
                     QIADQFKDQIPVILNLQTTDTELSKRLIDFASGLTYALDGGMQRIADKVFLLTPRNVE
                     VSAEERARLMEKGFFNQS"
     gene            4323..5126
                     /locus_tag="VF752_13270"
     CDS             4323..5126
                     /locus_tag="VF752_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856560.1"
                     /translation="MTDFNAQSGEVSIAGTDAGEGSPVLLLHGLTATRRYVVMGSKFL
                     ERNGYRAVAYDARGHGESSPGPSYDYSELAGDLGAVMDELQIEQAVVAGASMGAHTGV
                     RFALEHPARVAALVLITPAFDGSVNAEDENWDDLADGLERDGIDGFIAAMKPPSDPRW
                     HDTAITVARQRLERHRHLDAVAEALRQVPRSRPFEGLHALEEVDAPTLIVASRDESDP
                     GHPLRVAEAYHSRLRRSELMVEEPGKSPIAWQGAQLSRAIAEFAERAAA"
     gene            complement(5135..6007)
                     /locus_tag="VF752_13275"
     CDS             complement(5135..6007)
                     /locus_tag="VF752_13275"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8856561.1"
                     /translation="MASENLIGEYLRARRELVRPEDVGLSQIGGRRRVAGLRREEVAL
                     LAGVSVDYYVRLEQGRDQHPSEQVLDALARALQLDDHATGYLHRLAQPAPRRRRRPPR
                     REQVPPGIAQLIETWRETPAYVEGRYLDVLASNPLARALAPYYAVGTNLVRAVFFDPR
                     VQDMYEGFDKVTAMTVAALREVAGPDIDDPHLNELVGELSVRSERFRRLWARHDAVRR
                     GSGTARIHHPQVGLLELGYEKVPIPWADRQTLAMYHAEPGSPSAQGLALLASIAAGER
                     EPVAQPRSPATRRH"
     gene            6110..8161
                     /locus_tag="VF752_13280"
     CDS             6110..8161
                     /locus_tag="VF752_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856562.1"
                     /translation="MTSATPPPTALLTHEPTLASVWQAVAGRTVDDELLEWPPDVFAL
                     TNVLLARSEAYRFALSPPEELSWPPARIPGWADAVVDAARQWSAWVEDPIGALPELLA
                     EEWEILREAASMPLGDLTLARDWRCCEALLTVHAIADEACAGLAVALDSSNGEGFLYR
                     ARGRELLARTGSLTRIPSDFVRVLPKVLTPAHGTSLRSLSRYASVHGPGVETQWHKVR
                     GRRPGAAPYDKGVNYLLLPWPLRVRESDFRPLEGSVHSVAKEPFGFFEFAPSEGLDLD
                     LVDRTLVAAQDEVGLVNVVVLPESALDQSEIDPLEELLDRHGVIGLIAGVRHRADCPG
                     RFPANWVHLGISTGEHWTHICQGKHHRWSLDEDQIYQYHLGGALHPHVRWWEAMEVPR
                     RSVRFVEAGGATLVALVCEDLAQIDDVADVLRSVGPTLVVTPLLDGPQLSSRWTARYA
                     SVLADDPGSAVLTLTSFGMARRSRPRGRPSSSVVALWKDPGRGTREIALEDGAHGILI
                     STSTDRAARRSVDGRRPVDNASEVFDMSVYQVRAATAGSPPASSPAPSRSRAVLETED
                     LTILTSWAEAVAEAMACAPDRIDALPPDAGVGGQWRAELGIAEPSRRLDEAIGYMAGL
                     LRETDTTEAPLPLDALLRALIDHQPGENRLETLVRRVLRSALEQRQARRRHEGDRNMG
                     P"
     gene            complement(8180..8824)
                     /locus_tag="VF752_13285"
     CDS             complement(8180..8824)
                     /locus_tag="VF752_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HEX8856563.1"
                     /translation="MRQLFTGLRAERVRDNLERVRDRIAAAGREPAEVEVLAAIKYVP
                     IDELSVLAEAGIELVGENRAQDLLAKRERFADAFTWDFIGAVQSRKVRDIAPNVRLIH
                     SIASESALRQLERFPAPEILIEVNLAGEESKVGIEPGQIGDFIERSPVPVTGLMTMPP
                     FTESSEESRPHFARLAELAAEHGLSRLSMGTTQDYEVAVQEGATIVRLGTVLYE"
     gene            complement(8821..9531)
                     /locus_tag="VF752_13290"
     CDS             complement(8821..9531)
                     /locus_tag="VF752_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX8856564.1"
                     /translation="MIELSLPGARVVFSTRKGGVSSGPYESLNLGFLTDDEPSNVDAN
                     RERLVAAVGLEREQVAMGWQVHGAELLDWKKPPEHGGFAHLGAELPEVDGHLTAVPGL
                     GLLVLVADCLPVALSGDGRVAMLHCGWRPLAAGIVEKAVASFETPPAAVIGPGIGSCC
                     YEVGDEVFSAFSDLDGVASGRMLDLRAVTRRKLEAVGVTEIADVDMCTSCCPELFFSH
                     RRDGGVTGRQAGLVWREA"
     gene            complement(9528..10745)
                     /locus_tag="VF752_13295"
     CDS             complement(9528..10745)
                     /locus_tag="VF752_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856565.1"
                     /translation="MKERVEAIPGMERVIPALQGLPPAYLVGGAVRDLLLGIRSTDLD
                     VVVEGDAVAEARTLAERLGGEFVQHERFGTATVRADGLTLDLATTRRETYPEPGALPV
                     VEPAGLLEDLARRDFTVNAMAIGLTGDDEGVLHDPHGGREDLDRGTIRVLHERSFVDD
                     PTRLLRAVRYETRLGFSMDPETERLAREQAAGAGFSTVSGPRVRDELLDLLRERDLAT
                     GVERLHELGLDRALHPSIAADPGVAARARDAALEIGADPALAALASFLVPDTDAVAGW
                     VGALHVGRGAAAAVLRAARKGPQLVAALRNDLQPSAVHALLHCEPLEALALAVALGAP
                     ERPVLAYLDTLKDVKLDISGDDLVAAGVPQSPAIGRALRETLRLKLDGVVSGRDDELS
                     SALETARQWEADE"
     gene            complement(10742..11500)
                     /gene="hisF"
                     /locus_tag="VF752_13300"
     CDS             complement(10742..11500)
                     /gene="hisF"
                     /locus_tag="VF752_13300"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX8856566.1"
                     /translation="MLLKRVIPCLDVDKGRVVKGTNFVDIRDAGDPVELAVRYQDEGA
                     DEIVFLDITASHEKRETVAALARRTADDVFIPFTIGGGVRSVEDAQAVLDAGADKVSV
                     NSAAVARPELIAELAEVFGAQCVVLAVDARKRPEGGYEVFVNGGRTPVGRDAVDWIRE
                     GVERGAGEILLTSMDRDGTEDGYELELTRAASDGVDVPVIASGGAGRPEHLVDAVQEG
                     GADAVLCASIFHYGEYTVRQAKEAMRSAGVPVRL"
     gene            complement(11500..12228)
                     /gene="hisA"
                     /locus_tag="VF752_13305"
     CDS             complement(11500..12228)
                     /gene="hisA"
                     /locus_tag="VF752_13305"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX8856567.1"
                     /translation="MILLPAIDIRGGKAVRLERGDFSKETAYYDDPLEAARSFVDAGA
                     RFLHVVDLDGAREGEPMNLHHLRRITMDLEVPVQYGGGLRDVAAVRNALGAGAARVVV
                     GTAAYTDAEFLQTIVETWGRRVLVAVDVREDHVSVSGWTQATQMRPDELVSRLQLQGV
                     NQFVYTNADRDGLLGGPDLEEVKRISKVVRGRFLYSGGIASLDDLRALRQLRLLNLAG
                     VISGKALYEGLFTIDEAHDALKDD"
     gene            complement(12225..12851)
                     /gene="hisH"
                     /locus_tag="VF752_13310"
     CDS             complement(12225..12851)
                     /gene="hisH"
                     /locus_tag="VF752_13310"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX8856568.1"
                     /translation="MSSLAILDYGMGNLRSVEKAFAHVGVSARVTRDHEEISRAAGLV
                     LPGVGAFPKAMKAVRELGLDELVRSAGVPVLGICLGMQLLFEESAELGGATGLGLLRG
                     RVEPLDAPGLKVPQIGWNAVSWRKRTALNDGLGPETAFYHVHSFAPRPADDADVLGTA
                     TYGDEFASAVARGPVYGVQFHPEKSGPDGLRLLANFAAGCGLALSQAA"
     gene            complement(12848..13435)
                     /gene="hisB"
                     /locus_tag="VF752_13315"
     CDS             complement(12848..13435)
                     /gene="hisB"
                     /locus_tag="VF752_13315"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX8856569.1"
                     /translation="MTRSADISRTTNETDIRLSLALDGTGAGTRSTGVGFFDHLLDAV
                     AKHGALDLDVETKGDLETGPHHTVEDTGLALGAAIDEALGDRSGILRFGHAVVPMDDA
                     RASASIDISGRPYTAFEAQLPPVAIGSFDCDLTEEFFRAVANSAKLTLHVSVERGHNA
                     HHIVEASFKAFARALRQAVSLDPNESGVPSTKGLL"
     gene            complement(13432..14676)
                     /gene="hisD"
                     /locus_tag="VF752_13320"
     CDS             complement(13432..14676)
                     /gene="hisD"
                     /locus_tag="VF752_13320"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX8856570.1"
                     /translation="MRFGPDATAAQIRAIAPPADEPATAVAEILRSVRARGDAAVREL
                     TKQFDGTSLRDLRVPQAQLERARDSLHGGVRDGIEAAIANVRAAAEAQLRDPVAVELA
                     DGQRIEYVEVPVKRAAAYVPGGRAPYPSTVVMCAVSARVAGVDELAVCAPPGRQGRPD
                     DVILAACALCGVGEVYAMGGAQAIAALAYGTESVEAVDVIVGPGNRYVQEAKRQLAAA
                     RVGIDGVAGPSELVAVATRGADPELLALDLLAQAEHGPDGLLALISPDANLLDDTEQV
                     TVQLGEELASVHGAPLALVQAQDPEAAVRLADKLAPEHMELVGKEAESLASMVRCAGC
                     LFVGVQAGVAFGDYVAGSNHVLPTGGAARFASGLSPATFRRRMARVSLPGEAATRLAP
                     AGAALARAEGFPVHGESMERRA"
     gene            complement(14688..15329)
                     /gene="hisG"
                     /locus_tag="VF752_13325"
     CDS             complement(14688..15329)
                     /gene="hisG"
                     /locus_tag="VF752_13325"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX8856571.1"
                     /translation="MNGLTIAVPRGALFTETLDLLERLGIGTAEVRANERRLVFPEAG
                     LVTMRPSDVPTYVEHGAADIGITGKDVLLEQDEREVYELLDLGYGTCRMVVAEPEGEN
                     AIEESIRRLGAVRVATKYPRIAARHFADTGRQAEIVEVKGSVEIAPLTGLVAAIVDLT
                     ATGKTLVENRLVEREEIVVCTARLIANPVAHKLKAAAIDELVARIRSLDGVVA"
     gene            complement(15326..16315)
                     /gene="hisZ"
                     /locus_tag="VF752_13330"
     CDS             complement(15326..16315)
                     /gene="hisZ"
                     /locus_tag="VF752_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX8856572.1"
                     /translation="MIHPIPPGTRDVLPDEMRELRALSDGLRSEFERAGYGEVWTPTM
                     EYEDVLRRGDERAAGAGYRLFDEHGQVLALRSDSTIPIARLVASRYQDAEPPLRFCYF
                     AHAYREVHRGSAQAREFLQGGIELIGPSAPEGDAEMVALVSSALEEAGLRRHRIGLGD
                     ASLYRTLLADYGVPEDERMPLLERLSRRDLVGVGVSVERLGLPAPAREVLTTLPTLRG
                     GPEVLERVSDAHVAGAIENLRACHELLADRGIGDRVIFDLGLVRELGYYTGAVFEVYD
                     PAVGFALGGGGRYDDLLGRFGRPLPACGMALDLHRVHLAQAAEERLAREEAPA"
     gene            complement(16340..17644)
                     /gene="murA"
                     /locus_tag="VF752_13335"
     CDS             complement(16340..17644)
                     /gene="murA"
                     /locus_tag="VF752_13335"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8856573.1"
                     /translation="MEKFVIDGGYPLSGTIVPSGNKNAALPVLASCLLTEEDVVLHNI
                     PRIRDVEAMLGLLAALGVSVEWIADNSLRLNAANASAEPIDPDLSEKIRASFLLAGPL
                     LARFGRVHMPPPGGDVIGRRRLDPHLDAFRSLGAEVHHNRWCDLRAPHGLRAADFFLD
                     EPSVMATENALMAAALTPGSTVIHNSASEPHVQDLARILLQMGARIEGIGSNVMIVHG
                     QESLGGAEYRIGADYIEVGSFIALAAVTGGELRIRDVVRQDLRMTSLVFERLGVETLF
                     QGDDLVVPPGQRLRVRDDAGEQTATIQDGPWPAFPADLTSIALALATQADGLVLIHEK
                     MFENRLFFVDKLINMGARVILCDPHRAVVAGPRRLHGERMESPDIRAGMAMLIAALCA
                     DGTSEIGNIRQIDRGYERIDERLRLLGARIERVSSEEPVPAT"
     gene            17754..18338
                     /locus_tag="VF752_13340"
     CDS             17754..18338
                     /locus_tag="VF752_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX8856574.1"
                     /translation="MTVSRDEAWALFCEWTESESLRKHVLGVEAAMVAYARKWGEDEE
                     LWAATGILHDLDYERHPDLETGHPRVALEYFRDNGYPQELIDAVAGHATFLGVPRETR
                     MAKTLFAVDELTGFIAAVALVRPTGIHGLTPKSVKKKMKQPSFAAAVNRDELRQGAEE
                     LGVDFDEHVAFVIAAMEERADELGLGPREQEPAA"
     gene            complement(18335..18700)
                     /locus_tag="VF752_13345"
     CDS             complement(18335..18700)
                     /locus_tag="VF752_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856575.1"
                     /translation="MTSAGGTSAGNGRCSWCGTPVERDDGFRAAEPAGSRRAVFCRLE
                     HVVPWFIQGPHWDAGDLEEPSGIDADITLCAHCGTELGDTRVLLVRHRGEHRIPDAFC
                     SAAHLNEWAKAGGRWRPGG"
     gene            18719..19819
                     /locus_tag="VF752_13350"
     CDS             18719..19819
                     /locus_tag="VF752_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX8856576.1"
                     /translation="MAEARIIVLEGDETGQELLDQALRVLDPDVTKVDVELEHYDLSL
                     DNRRKTNNDVVHAAARAMKEARFGIKAATITPEGKDDVGSPNRLLREGIDGKVIIRTG
                     RRIPGVTPIAGVHFPISVVRMAVGDAYGAEQWRETEDGDEIAFRTERISRNVCTAVAE
                     YSFRTASKIHARVYGGPKWTVSPVYEGMLKEEMDAAAERYPDVPYQPVLIDATYAGLI
                     SGAADAPLVIPSLNRDGDCLSDFVLPLFGSIAGAESTLLAFDDDYQLTAVMAEAPHGT
                     APALLGKNIANPMAMIMAVAALLHYMAELGVAPQADLASRAVYEAVLEATASGVRTPD
                     LSGHATTSEFTDEVISRTRTKIDIWSSLGSTV"
     gene            19862..20326
                     /locus_tag="VF752_13355"
     CDS             19862..20326
                     /locus_tag="VF752_13355"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8856577.1"
                     /translation="MAREYVFVDEWDVDAPVESVFAALSDASTYPLWWKPVYIEVESD
                     GPPVVGRTSRQHFKGRLPYRLKTESRITELEPPRRLAVDVHGDLSGTGVWSLTPRDHG
                     VHVRFDWRVNADRPLLRYLTPVLRPLFRWNHNWAIARAREGLEPYARLAARR"
     gene            complement(20343..21299)
                     /locus_tag="VF752_13360"
     CDS             complement(20343..21299)
                     /locus_tag="VF752_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEX8856578.1"
                     /translation="MLIHLRIVAPEHCSNDVVELLEQSDSVTNVVHLRGVAAKPKGDL
                     ILSDVAREDASAVISRLRELPIEPHGSIAVDEPDAEIGEPFVRAEHAAAGLPSDAVVW
                     EMVRARTSEDSELSLSFLSFMALAMMIAAAGIFLDEPILIVGAMIVGPEFGPLAGVSV
                     ALVQRRGELAARSLKALLVGFPVGIVLTLAVTEIWKLIGLVPDSFDLEKRVLTEFIAN
                     PDWWSPVVAFLAGAAGVLSLTSAKSGALIGVLVSVTTIPAAANIAVAATYRDWDQVGG
                     AALQLIVNLTTIILAGVITLLVQREVQRAMRRRDERRRTIAT"
     gene            complement(21305..21742)
                     /locus_tag="VF752_13365"
     CDS             complement(21305..21742)
                     /locus_tag="VF752_13365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8856579.1"
                     /translation="MSQTFRHRLRVRYGECDPQDVVFNANYFAYFDVVLTEFWREAIG
                     PYGAMLELGADLVVAEANARFLGSARFDDEIDFELRITRLGSTAMTTRIDIRREGELL
                     VEGEMRHVFIDPATKRKREMPADIRRGLTPYVADGAELAEPAV"
BASE COUNT         3290 a         7954 c         7532 g         3217 t
ORIGIN      
        1 gcggcgctcc tgctcctcgc gctcctggcg cgcgcgctcc tcctcagcac ggaagatcgc
       61 cgcgcgctcg gcggcgacgc tgcctatcgc gtccacgatc agcttgcagg agcggatcgc
      121 gtcgtcgttg ccggggatcg ggtaggtgac cggatccggg tccacattgg tgtccaccag
      181 gccgatgatc gggatgcgca ggcgctgcgc ctcgcgcacg ccgattgcct cggtcttcag
      241 atcgaccacg aacaccgcgt ccggggtccg ctgcaggttg cgtacgcctc cgaggttcac
      301 ctcgagcttc tcgcgctcgg cgatcgccgc gatgcgctcg cgcgtcggca gcagctcgag
      361 cgtgccgttc tcggtccaat cggtgaggtc gtggagccgc ttgatgcgct tcgagatcgt
      421 ctggaagttg gtgagcagcc cgccgagcca gcgctgatcg atgtacggca tgccggcggc
      481 cgtggccgcc tccttcacag cgtcacgcgc ctgcttcttg gtgccgacga acaggatcgt
      541 gccgccgcgc gaggcaacct cgcccgcgaa gtcctgtgcc ctcttcagca gctgctcggt
      601 ctgctcgagg tcgatgatgt ggatgccgcc ccgctcgccg aagatgaatc ggcgcatctt
      661 ggggttccac cggcgcgtct ggtggccgaa gtgcacaccg gcctccagca gctccctgat
      721 tcctacttcc tgggccactg cttctccctt gtcgttctgg tgtggcgaaa tgcccgccgg
      781 cttcgtgtgc gcggacccgg ctatctgccg ggcagggccg cgttcccgct ggcggggtag
      841 ttggttgcgt gcagcgcgta aggataggca accgcgctca gaaagagacg aggcggcccg
      901 aaagccgccc cgctccgttc tatgttcagc ctccgtctgg cccttcggag gctggctccc
      961 gcttcgctgc gtcgaatgca cggcacagct cagcgggtag tttggactcg caacgcacat
     1021 tttccccccg agtcggatgc tcaaacatga gcgtcgagct gtggaggaat tggcgcgcga
     1081 gcccaaggcg cgcgccactg ggggctccgc cgtattgcgt atcgcccacc accgggtggc
     1141 cgatcgccgc catgtgagcg cgtatctggt gggtacggcc ggtctcgagg cgcaccctca
     1201 gcaggctggt gcggggcagc gcctcgacga gctcgaaatg cgtgaccgcg tcgcgcccgg
     1261 cgtctgtgtg ggtggagatg agggtgcgct cgcggcggtc gcggcccacg gcggcgtcga
     1321 tcatcccggt ccgcgaggcg ggccttccct ccacgagcgc gaggtactcg cgcgtgatct
     1381 tccgctcgcg gagcatctgc tggagcgcac gatgcacggg ctcggacttg gccaccacga
     1441 ggaggcccga ggtatcgcgg tcgagccggt gcacgatgcc ggggcgcagc gggtcgccgc
     1501 cggccgcgcc gcgcgccgct agggcctgtg ccagcgtgcc ctcggggtgg ccgggcgccg
     1561 ggtgcaccac cacgcccgcc ggcttgtcga ccaccattag atcgtcgtcc tcgtagacga
     1621 cctcgaacgg gacgtcggcg ccgccgcgcg agtcggccgg cgggcgctcc tcgatcagca
     1681 cctccaccgc ctcccccggg gcgaggtggt ggcgcttctg gcgcgcgcgt ccgtcgaccg
     1741 tcaccgcgcc ggactcgatc agccgctgcg ccttcgcgcg cgagccgatc gccggctcct
     1801 ctgcgagccg gacgtcgagg cggcggccgg cgtcgtcctc gcccgccgtg aaggcgacgc
     1861 gcgcgctcac gtgcggtgcg ggcgctcgat cacgtacagc aggccgagca ccccgaccac
     1921 gatgcagatg tcggcgaagt tgaacgcggg ccagaactcg gggtcgatga agtcggtgac
     1981 cgcgccgtcg cgggcccggt cccagagatt cccgagcgag ccgccgagca cgaggcctgt
     2041 cgggagccag agcagcggcg tggtggtgcg aaggacgaag tacaggagga gcgcgaccag
     2101 cgccaggctc gtgagcacgg tgacgatggc accgccgcca ccgaagaagc cgaaggcgac
     2161 gcccttgttc cgcacgtagc tcaggtcgat tccgaggaag agcttccgcg actctccgag
     2221 cgcgagcgac gagcgcacgg cggccttcgt ggcctggtcg ataccgatca cgagcacggt
     2281 cgtgaacagc gcgagcagcc aggcgcgaag ccgcgggctc atggcaggcg ctcccccgcc
     2341 gcggcggcgc tgaggccttc aaggcgcagc agcttgtcgc gcccggacgc gccgctcacg
     2401 agcgccaccc ggccgggcgg cacgccgaca atcttcgcca cgagcttccg gagcgcgtcg
     2461 ttcgcttttc cgtcgaccgg cggagcggtc acgcgcacga gcagggctcc cgcgcgctcg
     2521 ccgacgagct cgttccggct cgcaccggcc cggacgcgca cccacacgag cgccgagccg
     2581 tcgcgctccg acaggtactg ctgggatccg gccacggctt cagccggcga tcgccgacac
     2641 cacgatccgc tcgatgatga agagcacgaa gatcgcgatc gtcgggctca ggtcgatcgc
     2701 tcccgggccg atgcggatcg gcggaaggat tcgcctgaag acgttgagga acggatccgt
     2761 cacctccctc acgaacgtga ggaacatgtc gagccagcgg ttgtatggga tccgtggcag
     2821 ccagctcatc aggatccgta tgaagatcag gacgatgtag acgatgatca gcgtccccac
     2881 gtaggacgcg atgtcgtcgc gcgtgacggc gaggacgagg gtcatcgagt cgcctcgacc
     2941 accgcagcca ccgcgtcgtc gaaggcggtg cgcaggccgc cccgctcgag cgccgcgagg
     3001 ccgcgcgccg tcactccacc cggcgaggtc acgcgggtgc gcagggctgt ggcgtcgagg
     3061 tcgccgcgcc gcagcacctc cgcgcttccg cccatcgtct cgaccgtcag cttggccgcg
     3121 tcgtcgcgtg cgagcccttg cttcactccg gcctccacga gcgcctcgac cacgagcgcg
     3181 aagaaggcgg gaccggagct cattagcgcc atcgccggct cgatcagggg ctcggacggc
     3241 agccgcacca cctcgccgac tcgctcgaac agaccgatca cgtcggactc cgggccatcc
     3301 gaggcgagcc gccccggcac gtagcaggcc acgccctgct tcacctcgac tgggatgttc
     3361 ggcaggaagc gatacacggg ccgatcggga tacgcctcct cgacgcgggc gactggcgtc
     3421 gcagcgagga tcgacacgac cgccttggcg ttgtcgtgcg cctcggccgc gacctcgtcg
     3481 agctgcgccg gcttgtggca gaggaagacg acgtcggcct gctcagccac ctcggcattc
     3541 gaggcaagcg cctcgccgcc gagctcctct gcgagcgcct cggcgcgccc cgcgacgggg
     3601 tcgaacacga gcacgggctc gccaaatccg cgagcgagtg cgctcgccat gttgcctgcg
     3661 cctatgaggc caatccgcat ggcgcgcgaa gcataggcgg cgcgggcgcg ccggccctag
     3721 gactggttga agaagccctt ctccatcagc cgcgcgcgct cctccgcgga gacctccacg
     3781 ttgcgcggcg tgaggaggaa gaccttgtcg gcgatgcgct gcatgccgcc gtcgagcgcg
     3841 tacgtgaggc ccgaggcgaa gtcgatcagg cgcttggaca gctccgtgtc cgtggtctgg
     3901 aggttgagga tcaccgggat ctggtccttg aactggtcgg cgatctgctg cgcgtcgttg
     3961 aagttcttgg ggacgacgag gtggacctgc acgtccgagc ggccgttgcc gaccgggcgc
     4021 agcacgcgcg tctggcgccc gccgcggccc gcgggctcgt cgtcggcgaa gatgtcgtcg
     4081 aactcgtccc ggcgcctgcg gctctggagc cgtcgcacgt tcggacgctc gcgatagcgg
     4141 tcctcgagct cgtgctcggg ctcgagctcc tcctccgcgc ggtcgtaccc gtcatcctcc
     4201 tcggcgaggc cgaagtagac gagtgcgcgg tgccagctgt cgcgaagcgc catcgggtgc
     4261 tgagattcgc ccgggagcgg cgaaatcctc tcgtttacac gttacagcgt tctatcgccc
     4321 aaatgaccga tttcaatgcg caatccggcg aggtctcgat cgccgggacg gacgccggag
     4381 agggctctcc ggtgctcctg cttcacgggc tgaccgccac gcgccggtat gtcgtgatgg
     4441 ggtcgaagtt cctcgagagg aacgggtatc gcgcggtggc ctacgacgcc cgcggtcacg
     4501 gcgagtcgtc ccctgggcct tcctacgact attcggagct agccggggac ctcggggccg
     4561 taatggatga gctccagatc gagcaggcgg tagtagctgg ggcgtcaatg ggcgctcaca
     4621 cgggcgtgcg gttcgcgttg gaacacccgg cgcgcgtcgc agcattggtg ctcatcactc
     4681 ccgcgttcga cggctctgtc aatgccgagg atgagaactg ggacgatctc gcggacgggc
     4741 tcgagcgcga cggcatcgat ggcttcatcg ccgcgatgaa gcccccgtcc gacccgcgct
     4801 ggcacgacac cgcgatcacc gtggcccggc agcggctgga gcgccatcgg cacctcgacg
     4861 cggtcgcgga ggcgcttcgg caggtcccgc ggtcgcggcc gttcgagggg ctgcacgctc
     4921 tggaggaggt cgatgcgccc acgctcatcg tcgcgagccg cgacgagtcc gacccgggtc
     4981 acccgctgcg cgtggccgag gcttaccact cgcgcctgcg acgctccgag ctgatggtcg
     5041 aggagcccgg gaagtcaccg atcgcgtggc agggggcgca gctctcgagg gcgatcgcgg
     5101 agttcgcgga gcgggcggcg gcctgaggcc cagctcagtg ccgacgcgtt gcggggcttc
     5161 gaggctgcgc aacgggctca cgctcgccgg ctgcgatgct ggccagcagc gccagcccct
     5221 gagcgctggg gctgccgggc tccgcgtggt acatcgccag cgtctgccgg tccgcccacg
     5281 ggatcggcac cttctcgtag ccgagctcca acaggccgac ctgaggatga tggatcctcg
     5341 ccgtcccgct gccccggcga acggcgtcgt gccgcgccca gagccggcgg aagcgctcgc
     5401 tccggaccga cagctcaccg accagctcgt tcagatgagg atcatcgata tcggggccgg
     5461 cgacctcacg aagtgctgca accgtcatcg cggtgacctt gtcgaagccc tcgtacatgt
     5521 cctgcacgcg ggggtcgaag aagacggccc ggacgaggtt ggtgccgacc gcgtagtacg
     5581 gcgcaagcgc gcgcgccagc gggttcgacg ccagcacgtc caggtagcgg ccctcgacgt
     5641 aggccggggt ctcgcgccag gtctcgatga gctgagcgat gcccgggggc acctgttcgc
     5701 gccgcggagg cctacgacgc cgacgcggcg ccggctgggc gaggcggtgt aggtaaccgg
     5761 ttgcgtggtc gtcgagctgc agtgcacgcg cgagcgcgtc gaggacctgc tcggagggat
     5821 gctggtcacg gccctgctcg agccgcacgt agtagtcgac gctcacaccg gcaagcagcg
     5881 cgacctcttc gcgacgcagc cctgcaactc gccggcgtcc accgatctgg ctgagcccaa
     5941 cgtcctccgg ccggacgagc tcgcgtcgcg cgcgcaggta ctccccgatc aggttctctg
     6001 aggccacaca aaaaacgata cgcccgcggc ggagcccgat cgtggccctg ccactaccag
     6061 gatcaaggga cctcttgtgt gcgcgcgagc cgtatcgaac actggcttca tgaccagcgc
     6121 cacaccgccc ccgactgcgc tcctcacgca cgagcccaca ctggcctcgg tgtggcaggc
     6181 agtggccggg aggaccgtcg acgacgagct gctcgagtgg cctcccgatg tgttcgcgct
     6241 gaccaacgtg ctcctcgcgc gctcggaggc gtatcggttc gccctctccc cgcctgaaga
     6301 gctgtcgtgg ccgccggctc gaattcccgg gtgggccgac gcggtcgtcg acgcggcccg
     6361 gcagtggagc gcctgggtgg aggatcccat cggcgccctc cctgagctcc tggccgagga
     6421 atgggaaatc ctccgagaag cggccagcat gccgctcggc gatctcacgc tggcgcgcga
     6481 ctggcggtgc tgcgaggcgc tgctcacggt gcacgcgatc gcagacgaag catgcgccgg
     6541 ccttgccgtc gccctcgact cgtccaacgg agaggggttc ctctatcggg cgcgcgggcg
     6601 cgagctcctg gctaggacgg gatccctcac tcgcattcca tccgacttcg tccgcgtcct
     6661 gccgaaggtc ctgaccccgg cgcacggcac gtcgctgcgc tcgctctcac gctacgccag
     6721 cgtgcacggg ccgggtgtgg agacgcagtg gcacaaggta cgaggccgcc gcccgggggc
     6781 tgcgccctac gacaagggag tgaactacct gctgctgcca tggccgttgc gagtccgcga
     6841 gtcggacttc cgcccgctcg aggggtcggt gcacagcgtg gccaaggagc cattcgggtt
     6901 ctttgagttc gcgccgtcag aaggcctcga tctcgacctc gtggatcgga cgctcgtcgc
     6961 cgcacaggac gaggtcggac tcgtcaacgt cgtggtcctc ccggagagcg cattggacca
     7021 gagcgagatc gaccccctcg aggagctact ggaccgccac ggggtgatcg gcctgatcgc
     7081 cggcgtccgc caccgcgcgg attgcccggg ccggttcccg gccaactggg tccacctcgg
     7141 aatctcgacc ggggagcact ggacacacat ctgccagggc aagcaccacc gctggtccct
     7201 cgacgaggat cagatctacc agtaccacct gggaggggct ctgcaccctc acgtccgatg
     7261 gtgggaggcg atggaggtgc cgcgccggtc ggtgcgtttc gtagaagccg gcggcgccac
     7321 gctagtcgcg ctcgtgtgcg aggacctcgc gcagatcgac gacgtggccg acgtgctgcg
     7381 ctccgtcggg ccgacactgg tcgtaacccc cctgctcgac ggaccgcaac tcagctcccg
     7441 ctggacggca cgctacgcga gcgtgttggc cgacgatccc ggctcggccg tcctgacgct
     7501 gacgtccttc ggcatggcac gccgctcgcg accgcgcgga cgcccctcat cctccgtggt
     7561 cgcgctctgg aaggaccccg gccggggcac ccgcgagatc gcactcgagg atggcgccca
     7621 tggcatcctc atctcgacca gcaccgaccg agcggcccgg cggagcgtcg acgggcgccg
     7681 gcccgtggac aacgccagcg aggtgttcga catgagcgtc taccaagtcc gggctgcgac
     7741 ggctgggtcg ccgcccgcga gctccccggc gccctcacgt tcccgagccg tcctcgagac
     7801 cgaagacctc acgatcctca cgtcctgggc agaagcggtc gccgaagcga tggcgtgcgc
     7861 gcccgaccgc atcgatgcac tgccgcccga cgcgggtgtc ggcggccagt ggcgcgctga
     7921 gctcgggatc gcggagccct cccgacggct cgacgaagcg atcggctaca tggccggtct
     7981 cctccgagag acagacacga ccgaggcccc gctgccgctg gatgcgctcc tacgcgccct
     8041 catcgatcac cagccgggcg agaaccgcct cgaaacgctg gtccgccgcg tgctgcggtc
     8101 ggccctcgag cagcgccaag cgcgacgcag gcacgagggt gaccgaaaca tgggccctta
     8161 gcgggcgacg acgctcgcct cactcgtaca gcacggtgcc gaggcgcacg atcgtcgcgc
     8221 cttcctgcac cgcgacctcg tagtcctgcg tcgtgcccat cgacagccgc gagagcccgt
     8281 gctcggccgc cagctcggcc aggcgcgcga agtgcggccg gctctcctca ctgctctctg
     8341 taaacggtgg catcgtcatc agccctgtca caggaacagg cgagcgctcg atgaagtcgc
     8401 cgatctgacc cggctcaatc ccgaccttcg actcctcccc ggccaagttc acctcgatga
     8461 ggatctccgg cgccgggaag cgctcgagct gcctcagcgc cgactcgctc gcgatcgagt
     8521 ggatgagccg cacgttcggc gcgatgtccc gcaccttccg actctgcacg gcgccgatga
     8581 agtcccacgt gaacgcgtct gcgaaccgct cccgcttcgc aagaagatcc tgcgcacggt
     8641 tctcccccac gagctcgatc cccgcctcag cgagcacgct gagctcgtcg atcggcacgt
     8701 acttgatcgc cgctagcacc tcgacctcgg ccggctcacg gccggccgcg gcgatgcggt
     8761 ccctcacgcg ctcgaggttg tcccgcacgc gctcggcgcg caggcccgtg aacagctgcc
     8821 tcatgcctcc ctccacacga gcccggcctg gcgccccgtc acgccgccat cacgccggtg
     8881 ggagaagaac agctctgggc agcacgaggt gcacatgtcg acgtccgcaa tctcggtgac
     8941 cccgacggcc tccagcttgc gccgggtcac cgcgcgaagg tcgagcatcc ggcccgaggc
     9001 cactccgtca agatcggaaa aagcactgaa cacctcgtcg cccacctcgt aacagcacga
     9061 cccaatcccc gggcctatca ccgctgccgg aggcgtctcg aagctcgcaa ccgccttctc
     9121 gacgatgccc gccgccagcg ggcgccaccc acagtggagc attgcgaccc gaccatcacc
     9181 gctcagtgcc acgggcaggc agtccgccac gagcaccagc agcccaagtc cgggcacagc
     9241 agtcaggtgc ccgtcgacct cgggcagctc cgccccgagg tgagcgaaac caccgtgctc
     9301 tggcggcttc ttccagtcga gcagctcggc gccgtgcacc tgccacccca tagccacctg
     9361 ctcgcgctcc agccccaccg cggcgacaag ccgctcacgg ttcgcgtcca cgttcgacgg
     9421 ctcgtcgtcg gtgaggaagc cgaggttgag cgactcgtac gggcccgagc tgacgccgcc
     9481 cttgcgggtg gagaacacga cgcgggcacc cgggagagaa agctcgatca ctcgtccgcc
     9541 tcccactgcc gcgccgtctc gagcgcgctg ctcagctcgt cgtcgcgccc cgacaccacg
     9601 ccgtcgagct tcagccgcag cgtctcgcgc agtgcgcggc cgatcgccgg cgactgcggc
     9661 acgccagcgg ccaccaggtc gtcgccgctg atgtcgagct tcacatcctt cagtgtgtcg
     9721 agataggcga gcaccgggcg ctccggcgca ccgagcgcca ccgcgagcgc gagcgcctct
     9781 agcggctcgc agtggaggag ggcgtgcacc gccgacggct gcaggtcgtt gcgcagcgcc
     9841 gcgacgagct gcggtccctt acgggcagca cgcagcaccg cggcggccgc cccccggccc
     9901 acatgcagcg cgccgaccca cccggccacc gcgtccgtgt ccggcaccag gaacgaggcg
     9961 agcgcggcca gcgcgggatc tgcgccgatc tccagggcgg cgtcgcgggc gcgcgcggcc
    10021 acgcccgggt cggcggcaat cgacgggtga agcgcgcggt cgagcccgag ctcgtgcagc
    10081 cgctcgacgc cggtggccag gtcacgctcg cgcagcagat cgagcagctc gtcgcgcacg
    10141 cgcggccccg acacggtcga gaagccggcg cccgccgcct gctcgcgggc gagccgctcg
    10201 gtctccgggt ccatcgagaa gccgagtcgt gtctcatagc gaactgcgcg cagcagccgc
    10261 gtcgggtcgt cgacgaagct gcgctcgtgc agcacgcgga tcgtgccgcg gtccaggtcc
    10321 tcacgcccgc cgtgcgggtc gtgcagaacg ccctcgtcat cgcccgtcag cccgatcgcc
    10381 atcgcgttca ccgtgaagtc ccgccgggcg agatcttcga gcagccccgc cggctccacg
    10441 accggcagcg ccccgggctc gggatacgtc tcccgccgcg tggtggccag gtcgagcgtc
    10501 agcccgtccg cgcgcacggt cgcggtgccg aagcgctcgt gctgcacgaa ctcgccaccg
    10561 agccgctctg cgagcgtgcg cgcctcggcg acagcgtcgc cctccaccac cacgtcgagg
    10621 tcggtgcttc tgatgccgag cagaaggtcg cgcacggcgc cgccgacgag gtaggcgggc
    10681 ggcagcccct ggagcgcggg gatcacgcgc tccatgccgg gaatcgcctc gacccgctcc
    10741 ttcacagccg gaccggcacc ccggccgaac gcatcgcctc cttggcctgg cgcacggtgt
    10801 actcgccgta gtggaagatc gacgcgcaca gcacagcgtc cgcgccgccc tcctgcacgg
    10861 cgtcgaccag gtgctccggc cgccctgctc cgcccgacgc gatcaccggg acgtcgactc
    10921 cgtcggacgc cgcccgcgtc agttccagct cgtagccgtc ctccgtgccg tcgcggtcca
    10981 tactcgtcag caggatctcc ccggcgccgc gctccacccc ttcgcgaatc caatcgaccg
    11041 cgtcgcgccc aaccggcgtg cgcccaccgt tcacgaacac ctcgtagccg ccctccggac
    11101 gcttgcgagc gtcgacggcc agcaccacgc actgcgcgcc gaacacctcc gccagctcgg
    11161 cgatcagctc cggccgcgcc accgccgccg agttcaccga caccttgtcg gcgcccgcgt
    11221 ccagcacggc ctgggcgtcc tccacactgc gcacgccgcc gccgatcgtg aacgggatga
    11281 acacatcgtc ggcggtccgg cgcgccagcg ccgccacggt ctcgcgcttc tcgtgcgagg
    11341 ccgtgatgtc gaggaacaca atctcgtcgg cgccctcgtc ctggtagcgc acggccaact
    11401 ccaccgggtc gccggcgtcg cggatgtcca cgaagttcgt gcccttcacc acgcggccct
    11461 tgtccacgtc gaggcacgga atcacgcgct tgagcagcat cagtcgtctt tcaacgcgtc
    11521 gtgcgcttca tcgattgtga acaggccctc gtacagcgcc ttcccggaga tgacaccggc
    11581 aaggttcagg agccgcagct gtcgcagcgc ccgcaggtca tcgagtgacg cgattccgcc
    11641 cgagtagaga aagcggccgc ggaccacctt gctgattcgc ttgacctcct cgagatccgg
    11701 cccgcccaag aggccgtcgc ggtccgcgtt cgtgtagacg aactggttca caccctgaag
    11761 ctgcaggcgg gagaccagct cgtccggccg catctgggtc gcctgcgtcc agcccgacac
    11821 cgacacgtga tcctcgcgca cgtcgaccgc cacgagcacg cggcgccccc acgtctcgac
    11881 gatcgtctgg agaaactccg cgtcggtgta cgccgcagtg cctacgacca cgcgggccgc
    11941 gcccgccccg agcgcgttgc gcaccgcggc tacgtcgcgc aggccgccgc cgtattgcac
    12001 cggcacctcg aggtccatcg tgatccggcg caggtggtgc aggttcatcg gctcgccttc
    12061 acgcgctccg tcgaggtcca cgacgtgcag gaagcgcgcg cccgcgtcga cgaacgagcg
    12121 ggcggcctca agcgggtcgt cgtagtaggc ggtctccttc gagaagtcac cacgctcgag
    12181 ccgaaccgcc ttgcccccgc ggatgtcgat cgccgggaga aggatcatgc ggcctggctc
    12241 agcgcgaggc cgcagccggc cgcgaagttc gcgagcaggc gcaggccgtc aggccccgac
    12301 ttctcggggt ggaactgcac gccgtacacc ggtccgcgcg cgaccgcgct cgcgaattcg
    12361 tcgccatagg tggccgtgcc gagcacgtcg gcgtcgtccg cgggcctcgg cgcgaacgag
    12421 tgcacgtggt agaacgctgt ctcgggcccg agtccgtcgt tgagcgccgt ccgctttcgc
    12481 cacgagacgg cgttccagcc gatctgcgga accttcaggc cgggcgcgtc gagcggctcg
    12541 acgcggccgc gcagcaaacc gagtcccgtg gcgccgccga gctcggcgct ctcctcgaac
    12601 aggagttgca tgccgaggca gatgccgagc acgggcacgc ccgccgagcg gacgagctcg
    12661 tcgagcccga gctcgcgcac cgccttcatc gccttcggaa acgcacccac gccaggtagc
    12721 accagtccgg cggcacgcga gatctcctcg tggtctcgcg taacccgcgc cgagacgccc
    12781 acgtgcgcga aggccttctc gaccgagcgc aggttgccca tgccgtagtc gaggattgcg
    12841 agcgagctca cagcaggcct ttcgtggacg gcacgccgga ctcgttcgga tcgagtgaga
    12901 cagcttggcg caacgcccgc gcgaacgcct tgaacgacgc ctccacgatg tggtgcgcgt
    12961 tgtggccgcg ctcgacgctc acgtgcaacg tcagcttcgc gctgttggcc acggcgcgga
    13021 agaactcctc ggtcaggtcg caatcgaagc tgccgatcgc cacgggcgga agctgcgcct
    13081 cgaaagcggt gtacggacgg ccggagatgt cgatcgacgc gctcgcgcgc gcgtcgtcca
    13141 tcggcacgac ggcgtgcccg aagcgcagaa tgccgctccg gtcgccgagc gcctcgtcga
    13201 tggcggcacc gagcgccagc cccgtgtcct ccacggtgtg atgcggaccc gtctcgagat
    13261 cgcccttcgt ttccacgtcg aggtcgagcg caccgtgttt ggcgaccgcg tcgagcaggt
    13321 ggtcgaagaa gccgactccg gtgctgcgcg tacccgcgcc ggtgccgtcg agcgccagcg
    13381 agagtcggat gtcggtctcg ttcgtggtcc gggagatgtc cgcgctgcgc gtcacgcgcg
    13441 ccgctccatc gactcgccgt gtacagggaa cccctccgcg cgagcgagag ccgcgcccgc
    13501 gggcgcgagg cgggtggccg cctcgccggg caaagatacc cgagccatcc ggcgccggaa
    13561 tgtggccggc gagagtccac tcgcgaagcg cgcggcgccg ccggtgggca gcacgtggtt
    13621 cgagccggcc acgtagtcgc cgaacgcgac gccggcctga acacccacga acaggcagcc
    13681 cgcgcagcgc accatcgagg ccaggctctc ggcctccttg cccacgagct ccatgtgctc
    13741 aggggcgagc ttgtccgcga gccgcacggc ggcctcgggg tcctgcgcct gcacgagcgc
    13801 gagcggggcg ccgtgcacgc tcgcgagctc ctcgccgagc tggaccgtca cctgctcggt
    13861 gtcgtcgagc aggtttgcgt cgggcgagat cagcgcgagc aggccgtccg gcccgtgctc
    13921 cgcttgggcg agcaggtcga gcgcgaggag ctcgggatcg gctccgcgcg tggccacggc
    13981 caccagttcg ctcgggcccg caacgccgtc gattcccacg cgcgcggccg cgagctgtcg
    14041 ctttgcctcc tgcacgtagc ggttgccggg gccgacgatc acatcgacgg cttccacgct
    14101 ttcggtgccg tacgcgagcg ccgcgatcgc ctgcgcgccg cccatcgcgt agacctcgcc
    14161 cacgccgcag agtgcgcagg cggcgaggat cacgtcgtcg gggcggccct gccggccggg
    14221 cggggcacaa accgcgagct cgtccacgcc cgcaacgcgt gcgctgaccg cgcacatgac
    14281 gacggtcgac ggataaggcg cccggccgcc cggcacatag gccgcggcgc gcttcaccgg
    14341 cacttccacg tactcgatgc gctggccgtc agccagctcg accgccacgg gatcccgcag
    14401 ctgggcctcg gcggccgcgc gcacgtttgc gatcgcagcc tcgataccgt cgcgcactcc
    14461 gccgtgcagg gagtcgcgcg cgcgctcgag ctgtgcctgt ggaacacgga ggtctcgcag
    14521 cgaagttccg tcgaactgct tcgtgagctc gcgcaccgcg gcatcgccac gcgcgcgcac
    14581 gctgcggagg atctcggcca cggccgtagc cggctcgtcc gccggcggcg cgatggcacg
    14641 gatctgcgcc gcggtcgcgt ccggtccaaa tctcaccgcg cgcaggttca tgccaccacc
    14701 ccgtcgagtg agcgaatgcg cgcgaccagc tcgtcgatcg ccgccgcctt gagcttgtgg
    14761 gcgaccgggt tcgcgatcag ccgcgccgtg cagaccacga tctcctcgcg ctccaccagc
    14821 cggttctcca cgagcgtctt gccggtggcg gtgagatcca cgatcgcggc gacgaggccg
    14881 gtcagcggcg cgatctcaac ggagcccttc acctccacga tctcggcctg gcggccggtg
    14941 tcggcgaagt gacgcgcggc gatacgcggg tacttggtgg cgacccgcac cgcgccgagc
    15001 cgcctgatcg actcctcgat cgcgttctcg ccctccggct ctgccaccac catgcggcag
    15061 gtgccgtagc cgaggtcgag cagctcgtac acctcgcgct cgtcctgctc gagcagcaca
    15121 tccttgccgg tgatgccgat gtccgcggcg ccgtgctcca cgtaggtggg cacgtcgctc
    15181 gggcgcatgg tcacgaggcc ggcctccggg aacaccagcc gccgctcgtt ggcgcgcact
    15241 tccgctgtgc cgatgcccag gcgctcgagc aggtcgagcg tctcggtgaa gagcgcgccg
    15301 cgcgggaccg cgatcgtcag cccgttcacg ccggcgcctc ctcgcgcgcg aggcgctctt
    15361 cggcggcctg ggccagatgg acgcggtgga gatccagcgc catgccgcac gcgggcagcg
    15421 gacgcccgaa gcgcccgagc aaatcgtcgt agcggccccc accaccgagg gcgaagccga
    15481 cggctgggtc gtacacctcg aagaccgcgc ccgtgtaata gccgagctcg cgcaccagcc
    15541 cgaggtcgaa gatcacgcga tcgccgatcc cgcggtcggc gagcagctcg tggcaggcgc
    15601 gcaggttctc gatcgcgccc gcaacgtgcg cgtcactcac ccgctcgagc acctcgggtc
    15661 cgccgcgcag cgtgggcagc gtcgtgagca cttcgcgtgc aggcgccggc agcccgagcc
    15721 gctccacgct cacgccgacg cccacgaggt cgcgtcgcga gagccgctcg agcagcggca
    15781 tgcgctcgtc ctcgggcacg ccgtagtcgg cgagcagcgt ccggtaaagc gacgcgtcgc
    15841 cgagcccgat gcggtggcgg cgcagtcccg cctcctcgag cgccgacgac acgagcgcca
    15901 ccatctcggc gtcgccctcg ggcgcgcttg ggccgatcag ctcgatgccg ccctgcagga
    15961 actcgcgcgc ctgcgcgctg ccgcggtgca cctcgcggta ggcgtgcgcg aagtaacaga
    16021 agcgcagggg cggctcggcg tcctggtagc gcgacgcgac caggcgcgcg atcggaatgg
    16081 tcgagtccga gcggagcgcg agcacctggc cgtgctcgtc gaacaggcgg tagccggcgc
    16141 cggccgcacg ctcgtcgccg cggcgcagca cgtcctcgta ctccatcgtc ggcgtccaca
    16201 cctcgccgta gccggcgcgc tcgaactcgg agcgcaggcc gtcgctcagc gcgcgcagct
    16261 cgcgcatctc gtctggcagg acgtcgcggg tccctggagg gatcgggtgg atcatgagcc
    16321 ccccggagag taggccggac tacgtcgcgg ggaccggctc ttcgctcgaa acgcgctcga
    16381 tgcgcgcccc gagcagccgc aggcgctcgt cgatgcgctc gtagccgcgg tcgatctggc
    16441 ggatgttgcc gatctcggat gtgccatcgg cgcacagcgc cgcgatgagc atcgccatgc
    16501 cggcacggat gtcggggctc tccatgcgct caccgtgaag acgcctcggg ccagctacga
    16561 ccgcgcggtg cgggtcgcac aggatcaccc gagcccccat gttgatcagc ttgtccacga
    16621 aaaacagtcg gttctcgaac atcttctcgt gaatgagtac caggccgtcg gcctgcgtgg
    16681 cgagggcgag cgcgatgctc gtgaggtcgg ccgggaacgc gggccacggt ccgtcctgga
    16741 tcgtggcggt ctgctcgccc gcgtcgtcgc gcacgcgcag gcgctgcccg ggaggcacga
    16801 ccaggtcgtc gccctggaag agcgtctcca cgccgaggcg ctcgaacacg agcgaggtca
    16861 tgcgcaggtc ctggcgcacc acgtcgcgga tgcgcagctc gccgcctgtc acagcggcca
    16921 gcgcgatgaa gctgcccacc tcgatgtagt cggccccgat gcggtactcg gcgccgccca
    16981 ggctctcctg gccgtgcacg atcatcacgt tcgagccgat gccctcgatg cgcgcgccca
    17041 tctgaagcag gatgcgtgcg agatcctgca cgtgcggctc ggacgcgctg ttgtggatca
    17101 cggtcgagcc gggtgtgagc gccgcggcca tcagggcgtt ctcggtcgcc atcacggacg
    17161 gctcgtcgag gaagaagtcg gctgcgcgca ggccgtgcgg cgcgcgcagg tcgcaccagc
    17221 ggttgtggtg cacctccgcg ccgagcgaac ggaaggcgtc gaggtgcgga tcgagccggc
    17281 ggcggccgat cacgtcgccg cccggcggcg gcatgtgcac gcggccgaag cgggcaagga
    17341 gcggccccgc gagcaggaag gaggcgcgga tcttctccga gaggtccggg tcgatcggct
    17401 cggccgaggc gttcgcggcg ttgagccgca ggctgttgtc ggcgatccac tccacgctca
    17461 cgccgagagc ggccaggagg ccgagcatgg cctccacgtc gcggatgcgc gggatgttgt
    17521 ggaggaccac gtcctcctcg gtcagcaggc acgaggcgag gaccggcagg gcggcgttct
    17581 tgttgcccga cgggacaatc gtccctgaga gcgggtagcc gccgtcgatt acgaacttct
    17641 ccatgtgaga gcgtctcgag ccggggtcgg acttcggccg cgcaagggta gcgaaggcca
    17701 tccgctggac tttcgccttc tacgggtgca gcccttccgc tagcgtggcg gccatgacgg
    17761 tatctagaga cgaggcatgg gcgttgttct gtgagtggac cgagtcggag tcgctccgca
    17821 agcacgtcct cggcgtcgag gcggcgatgg tcgcctacgc gcgcaagtgg ggcgaggacg
    17881 aggagctctg ggcggcgacc ggcatcctcc acgacctcga ctacgaacgc catcccgacc
    17941 tcgagaccgg gcacccccgc gtcgcgctcg agtacttccg cgacaacggc tatccgcagg
    18001 agctcatcga cgcggtcgcc ggccacgcca ccttcctcgg cgtgccgcgc gagacgcgta
    18061 tggcgaagac gctgttcgcg gtcgacgagc tcaccgggtt catcgccgcc gtcgcgctcg
    18121 tgcgcccgac cggcattcac gggctcacgc ccaagtccgt gaagaagaag atgaagcagc
    18181 cgagcttcgc ggcggccgtc aaccgcgacg agctgcgcca gggagccgag gagctgggcg
    18241 tggacttcga cgagcacgtg gcgttcgtga tcgccgcgat ggaggagcga gcggacgagc
    18301 tcggcctcgg tccgcgcgag caggagccgg ccgcctagcc gcccgggcgc cagcgcccgc
    18361 cggccttcgc ccactcgttc aggtgcgcgg cgctgcagaa ggcgtcgggg atgcggtgct
    18421 cgccgcggtg gcgcacgagc agcacgcgcg tgtcgccgag ctcggtgccg cagtgagcgc
    18481 agagcgttat gtcggcatcg attccgctcg gctcctcgag gtcgccggcg tcccagtgcg
    18541 ggccctggat gaaccacggc acgacgtgct cgaggcggca gaacaccgcc cgtcgcgacc
    18601 cggcgggctc ggccgcgcgg aagccgtcgt cgcgctcgac gggcgtgccg caccagctgc
    18661 agcgcccgtt cccggctgat gtgccgcccg cgctcgtcat gggtacagtc cctccccgat
    18721 ggctgaggcg cgaattatcg tcctggaggg cgatgagacg gggcaggagc tgctcgacca
    18781 ggcgctccgc gtgctcgacc cggatgtgac gaaggtcgac gtcgagctag agcactacga
    18841 cctctcgctc gacaaccgcc gcaagaccaa caacgacgtc gtgcatgcgg ccgcgcgagc
    18901 gatgaaggag gcccgcttcg gcatcaaggc ggcgacgatc acccccgagg gcaaggacga
    18961 cgtgggcagc ccgaaccgcc tgctgcgcga ggggattgac ggcaaggtga tcatccgcac
    19021 cgggcgccgg attccgggcg tgaccccgat cgcaggcgtg cacttcccga tctcggtggt
    19081 gcgcatggcc gtgggcgacg cctacggggc ggagcagtgg cgcgagacgg aggacggcga
    19141 cgagatcgcc tttcgcaccg agcggatcag ccgcaacgtg tgcaccgccg tggcggaata
    19201 cagcttccgg acggcgagca agatccacgc gcgcgtctac ggcggtccga agtggacggt
    19261 gagcccggtc tacgagggca tgctgaagga ggagatggac gcggccgccg agcgctaccc
    19321 ggacgtgccg taccagcccg tgctgatcga cgcgacctac gcaggcctga tctcgggcgc
    19381 cgcggacgcc ccgctcgtga tcccctcgct caaccgcgac ggcgactgcc tctccgactt
    19441 cgtgctgccg ctgttcggct cgatcgcggg cgcggaatcc acgctgctgg ccttcgacga
    19501 cgactaccag ctcaccgcgg tgatggcgga ggcgccgcat ggcaccgccc ccgcgctgct
    19561 gggcaagaac atcgccaacc cgatggcgat gatcatggcg gtggccgccc tgctccacta
    19621 catggccgag ctcggcgtcg cgccgcaggc cgatctcgca tcgcgcgccg tttacgaagc
    19681 cgtgctcgag gcgaccgcct ccggcgtgcg cacgccggat ctcagtggcc acgccaccac
    19741 cagcgagttc accgacgagg tgatctcgcg cacgcgcacg aagatcgaca tctggtcgtc
    19801 gctcggctcg acggtctgac gccgatgatc cggccggccg cctcccgcga agagtgtggt
    19861 catggcccgt gagtacgtct tcgtcgacga gtgggacgtg gacgcgccgg tcgagtccgt
    19921 cttcgccgcg ctctcagacg cctcgaccta tccgctgtgg tggaagccgg tctacatcga
    19981 ggtcgagtcc gacgggccgc cggtcgtggg ccggacttcg cgccagcact tcaagggccg
    20041 cctgccgtac cggctgaaga ccgagtcgcg gatcacggag ctcgagccgc cccggcgcct
    20101 cgccgtcgat gtccacggcg acctgtcggg aaccggcgtg tggtcgctca cgccgcggga
    20161 ccacggcgtg cacgtgcggt tcgactggcg ggtgaacgcc gaccggccgc tcttgcgcta
    20221 tctcacgcca gtgctgcgcc cgctgttccg ctggaaccac aactgggcga tcgcgcgggc
    20281 acgcgagggg ctcgagccgt acgcgcggct ggcggcgcga aggtaagacg caccccggct
    20341 cgctacgtcg cgatcgtgcg gcggcgctcg tcgcgccttc gcatggcgcg ttgcacttcg
    20401 cgctgcacca gcaaggtgat cacgccggcc aggatgatcg tcgtgaggtt cacgatgagc
    20461 tgcagcgctg cgccgcctac ctggtcccag tcgcggtagg tggcggcgac cgcgatgttg
    20521 gcggcggccg ggatggtcgt caccgacacc agcacgccga tcagggcgcc ggacttcgcc
    20581 gacgtgagcg acagaactcc ggcggcgccg gcgaggaacg ccacgaccgg cgaccaccag
    20641 tccgggttgg cgatgaactc ggtgagcacg cgcttctcga ggtcgaagct gtcggggacg
    20701 aggccaatga gcttccagat ctcggtgacc gcgagggtga gcacgatgcc gaccggaaag
    20761 ccgacgagca gcgccttcag cgagcgcgcc gcaagctcgc cgcggcgctg cacaagagca
    20821 acggaaaccc ctgcaagcgg cccaaattcg gggccgacga tcatcgcgcc gacgatcagg
    20881 atcggctcgt cgaggaagat gcccgccgcc gcgatcatca tcgcgagcgc catgaacgac
    20941 aggaagctga gtgagagctc tgagtcctcg gacgtgcgcg cccgcaccat ctcccacacc
    21001 acggcgtcgg aaggcagtcc ggccgccgcg tgctcggcgc gcacgaacgg ctcgccgatc
    21061 tcggcgtcag gctcgtcgac cgcgatcgac ccgtgcggct cgatcgggag ctcgcgcagg
    21121 cgggagatca ccgcgcttgc gtcctcgcgc gcgacgtcac tgaggatcaa gtcgcccttc
    21181 ggcttcgcgg ccacgccgcg gaggtgcacg acgttggtta ccgagtcgga ctgttcgagg
    21241 agctcgacca cgtcgttcga gcagtgctcg ggcgcgacga tcctcaggtg aatgagcacg
    21301 ccggttagac cgcgggctcg gccagctccg ccccgtcggc cacgtacggt gtgagcccgc
    21361 ggcggatgtc ggccggcatc tcgcgcttgc gcttggtggc cggatcgatg aacacgtgtc
    21421 gcatctcgcc ctccacgagg agctcgccct cgcggcggat gtcgattctc gtcgtcatgg
    21481 cagtgctacc gaggcgcgtg atccggagct cgaagtcgat ctcgtcgtcg aagcgtgcgg
    21541 accccaggaa gcgcgcgttc gcctcagcca ccacgaggtc ggctcccagc tcgagcatcg
    21601 cgccgtacgg tccgatcgcc tcgcgccaga actcggtgag caccacgtcg aagtacgcga
    21661 agtagttcgc gttgaacacg acgtcctgcg ggtcgcactc gccgtagcgc acacgcagtc
    21721 ggtggcggaa ggtctggctc atcgctccgc agcctatcca gcccgctcgc cagagcctct
    21781 gcaacgaatc ttgcagctca gagccccttt ccgtccgccc ccctccgtac cttccccgaa
    21841 cacatgttcc ccaccccctc agagctcggt ttctccctca ggcgggcatt cggcctcgcg
    21901 ctggagttcg ccaccctggg tgagctcgtg tacgagccca ccgagcctcg gccgcccgca
    21961 acgccccgca acgcggccgt tcgaagggag tcc
//
LOCUS       DASVXS010000236         5593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_209177, whole genome shotgun sequence.
ACCESSION   DASVXS010000236 DASVXS010000000
VERSION     DASVXS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209177"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VF752_13370"
     CDS             <1..423
                     /locus_tag="VF752_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8856580.1"
                     /translation="FGPKSISIKAGDTVTWTNTGQAPHDASGSGFKIPVLMTGKSASH
                     TFNQAGTFSYICSIHPFMKGTVVVAGVGDTGGGGGGSSGGSSNSGAPSANSGTASGPP
                     STTGQLPNTGLGLPRSALTGLIMLGLGLALRRGLKRAA"
     gene            560..1021
                     /locus_tag="VF752_13375"
     CDS             560..1021
                     /locus_tag="VF752_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856581.1"
                     /translation="MKHLSHPTRSSLAQLARYLGALALLGVGIAHIQQYYADSYSAVP
                     TIGTLFALNFASAAVVSLGLIAPLGRIAGRWATSIRAVLAVSGIGIAAGSLAGLLISE
                     NGGLFGFQEVGYRQAIVISIVLEAATIILLSVYLAVNGLGVTRREGRLSPA"
     gene            1045..1380
                     /locus_tag="VF752_13380"
     CDS             1045..1380
                     /locus_tag="VF752_13380"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX8856582.1"
                     /translation="MAYDEELANRIREVIASEEGITEKRMFGGLAFLIEGNMSVSASG
                     QGGLLLRVDPADTDELLSKPHAHPFEMRGRAMQGWLRVDADGVQTKRDLKRWVDRGIA
                     YARSLPPKG"
     gene            complement(1456..1848)
                     /locus_tag="VF752_13385"
     CDS             complement(1456..1848)
                     /locus_tag="VF752_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856583.1"
                     /translation="MTQTKTRIKKVGTVVIPVADQDREIDFYVDKLGFEKRADVPFGN
                     GYRWVEVAPAEADTTIALAPPPEGKPTGNVETGIGLQTDDIDALHGELKDRGVDVDPE
                     VSRMGDPVPPLFWLRDPEGNTLMVVEVS"
     gene            complement(1903..3369)
                     /locus_tag="VF752_13390"
     CDS             complement(1903..3369)
                     /locus_tag="VF752_13390"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX8856584.1"
                     /translation="MLALAAAYFLAGRASLALQYTGPVAAIWLPVGVGVATLYLAGLR
                     WLPGVLIGDLALADASQPLGTVLGITAGNMADIVVIALLLRRLLGPRAALDRLPQVGA
                     LLVAIGAGAAITATVATVSSLAGDLFGSSEISVFWRSWFLADASGSLVVIPLALAWAQ
                     PRSPAWPDRSGREEALTIAAVVGLSVIALSSDLPLTYMVFPALIWAALRFGQRGATLA
                     VAVAAVTTVWMTAHDVGAFVQHSITDRALSTQLYIAVAALTTLCLAAIVTERRRAALE
                     LASSHARIAAAGAEERRRLEQELHDSAQNRLVGLQVRMRLTQEQIEQTAPEVAARLDE
                     LLKDTEAVSDELRRIAHGLSPALLATSGVVGALQNECAHSAIRVHIAARDIGRSAPEV
                     ETAMYLSCLESIQNAAKHAGGRASVTVTLRRGEDELAFSVHDTGRGFDPTATAPGAGL
                     TSVRDRIETIGGRVEITAAPGRGTTVAGVVPWPPVARD"
     gene            complement(3527..4237)
                     /locus_tag="VF752_13395"
     CDS             complement(3527..4237)
                     /locus_tag="VF752_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856585.1"
                     /translation="MLNPLGASPPGSVWWRAVNERLLRDGCETVALLGGSAGEPSSQA
                     VRLWLEFGERPTGRNWYRAHNASVVGAYLEHRKLAEAESSPERFFMNVALARVLYAHA
                     LNAAPRLALGGLAPVGRLVGDPRLGMAGVFLSLRRVLPDRYPLTLDVERYIEGEQRLG
                     RMLDYAVIVPRLQHLYEWSADELSDPRVLGLVRDGNPIYAWPFEERHVWRSRHMPFAA
                     RVLERVTRPLAQTRAFRA"
     gene            complement(4479..5474)
                     /locus_tag="VF752_13400"
     CDS             complement(4479..5474)
                     /locus_tag="VF752_13400"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX8856586.1"
                     /translation="MSAAPARVAPEGDQLQAQRDAPVGGQAVLEGVMMRGVGHWAVAV
                     RKPVPEDAPDDVVLGEIAVEEFPLTSVLKKHRLLRLPIVRGVVALVESLGIGFKALGI
                     SANAQVPEGEEEVGRGAWFGTVLFSVLFAIGLFFILPAGVTNLFKDALPNSFVFVLIE
                     KLIRISIFVLYLWLISRLADLQRVFEYHGAEHKTISCFEAGLPLTPQNAKRFSRLHPR
                     CGTSFLLIVMIVAFFVFAPLGTPVWYWLFASRVIGIPLVAGLAFEIIKLFGKNRQKRW
                     ARALMWPGMQLQKLTTREPDLSQLAVAIAALEAVLAVENPADASDEEKVGMEVVA"
     gene            complement(5482..>5593)
                     /gene="rpmE"
                     /locus_tag="VF752_13405"
     CDS             complement(5482..>5593)
                     /gene="rpmE"
                     /locus_tag="VF752_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX8856587.1"
                     /translation="EICSNCHPFYTGRQKLVDTGGRVDRFRRRAAKASSK"
BASE COUNT          915 a         1932 c         1937 g          809 t
ORIGIN      
        1 ttcggtccga agtcgatcag catcaaggcc ggcgacacgg tgacgtggac caacaccggc
       61 caagcgccgc acgacgccag cggaagcggc ttcaagatcc ccgttctcat gactgggaag
      121 agcgcctcgc acacgttcaa ccaggccggg accttcagct acatctgctc gatccacccg
      181 ttcatgaagg gcaccgtcgt ggtggccggg gtgggcgaca ctggcggagg cggcggcggg
      241 agctcgggtg gctcctccaa ctccggcgcg ccctccgcga acagcggaac cgcgtccggg
      301 ccgccatcga cgaccgggca gctgccgaac accggcctcg gcttgccgag gtcggcgttg
      361 accggtctca tcatgctggg cctcggactg gcactgcgcc gcgggctgaa gcgcgcggcc
      421 tagcgcggca cctacctccg aggtaatcgg cacataaacg ctgcgctcgg gtcgcgcccg
      481 gcgggacgat tcgcccgcgc acggtcgtct taccttgcgt aagtcgatcc cgaagagcgc
      541 gtccctcact ggagccccca tgaaacacct gtcacaccca acccgatcat cactggcgca
      601 gctcgcgcgt tacctcggcg cgctcgcgct cctcggggtg ggcatcgctc acatccagca
      661 gtactacgcc gattcctatt ccgccgtgcc gaccatcggc accctgttcg cgctgaactt
      721 cgcgtcggcg gcggtcgtgt cgcttggcct gatcgcaccc ctcggacgca ttgcagggcg
      781 ctgggcgact tcgatccgcg ccgtgctcgc agtgagcggg atcggaatcg ccgcagggtc
      841 actggccgga ctcctcatca gcgagaacgg cgggctcttc ggtttccagg aggttggcta
      901 tcgacaggca atcgtgatct cgatagtgct cgaagccgcg acgatcatcc tgctgagcgt
      961 gtacctcgcg gtgaacgggc tcggagtcac acgtcgagag ggtcggctca gcccggcctg
     1021 atccagcccg gagataatcg gcctgtggca tacgacgaag agctggccaa ccggatccgt
     1081 gaggtcatcg cctccgagga gggcatcacc gagaagcgca tgttcggtgg cttggccttc
     1141 ctgatcgaag gcaacatgtc ggtgagcgcg agtggccagg gcggactgct gttgcgtgtc
     1201 gatcccgccg ataccgacga gctgctcagc aagccgcacg ctcacccctt cgagatgcgc
     1261 gggcgtgcga tgcaggggtg gctacgggtc gacgccgacg gcgtgcagac caagcgagac
     1321 ctcaaacgat gggtcgatcg cgggatcgcg tacgcgcggt cgctgccgcc gaagggatag
     1381 ctcgcctaga ggaccctgcc tcggccctcg ggctcggtgc agcagccgcg cccgaggccg
     1441 gagcaggaac ggaggtcagc tgacctcgac gaccatgagg gtgttcccct ccgggtcgcg
     1501 gagccagaaa agaggcggga cggggtcgcc catgcggctg acctcgggat cgacgtccac
     1561 gccgcgatcc ttcagttcgc cgtggagcgc atcgatgtcg tcggtctgga gaccgatgcc
     1621 cgtctccacg ttgccggtcg gcttgccctc gggcggagga gcaagggcga tcgtggtgtc
     1681 cgcctcggcc ggtgccacct cgacccagcg gtagccgttg ccgaagggca cgtcggcgcg
     1741 cttctcgaag cccagcttgt cgacgtagaa gtcgatctcc cggtcctggt cggcgaccgg
     1801 aatcaccacg gttcccacct tcttgatgcg agtcttggtc tgggtcacga cgttcctcct
     1861 ggggtttgga tcacacccct cagaccgcca gatgtggcga aactaatcgc gcgcgacggg
     1921 tggccatggg acgacgccgg cgaccgtagt gccgcggcct ggcgccgccg tgatctcgac
     1981 gcgcccgcca atggtctcaa tgcggtcacg gacgctggtg aggcccgcgc cgggggccgt
     2041 ggccgtcggg tcgaagccgc gcccggtgtc gtggacgctg aaggcaagct cgtcctctcc
     2101 gcgtcgcagc gtgacggtca ccgaggcgcg accgccggcg tgtttcgccg cgttctggat
     2161 cgactcgagg caggacaggt acatcgcggt ctcgacctcg ggcgcgctcc ggccgatgtc
     2221 gcgggcggcg atgtgcactc tgatcgcgct gtgggcgcat tcgttttgca gtgcgccgac
     2281 gacgccgctt gtggccagca gcgccggcga aaggccgtgc gcgatgcgcc gcaactcgtc
     2341 gctgacggcc tcggtgtctt tgaggagctc atccagcctg gcggcgacct cgggcgcggt
     2401 ctgctctatc tgctcctggg tcagccgcat ccggacctgc aggccgacga gacggttctg
     2461 ggcgctgtcg tgcagctcct gctccagccg ccggcgctcc tccgcgcccg ccgccgcgat
     2521 ccgcgcgtgc gagctggcca gctccagcgc ggcgcggcga cgctcggtga cgatcgcagc
     2581 caggcagagc gtcgtgagcg ccgcgaccgc aatgtagagc tgggtactca gcgcgcgatc
     2641 cgtgatcgag tgctgcacga acgcgccgac gtcgtgggcc gtcatccaca ccgtcgtaac
     2701 ggcggcgacc gccacggcta gcgtggcgcc gcgctggcca aagcgcaacg ccgcccagat
     2761 cagtgccggg aacaccatgt aggtcagcgg caggtcgctc gacagcgcga tcacgctgag
     2821 cccaacaacc gccgcgatcg tcagcgcctc ctcccgaccc gagcgatccg gccatgccgg
     2881 ggagcgcggc tgcgcccacg ccagcgccag tgggataacg accagcgagc ccgacgcatc
     2941 cgccaggaac caactgcgcc agaacaccga gatctcggag gacccgaaga ggtcgcccgc
     3001 tagcgaggag accgtcgcga cggtcgcggt gatcgccgcg ccggccccga tcgcgacaag
     3061 gagagcgccg acctgcggga ggcgatccag cgcggcccgc gggccgagca aacgccgcag
     3121 cagcagcgcg attaccacga tgtcggccat gttgcccgcc gtgattccga gcacggtgcc
     3181 gagcggctgc gaggcgtcgg ccagggctag gtcaccgatg agcacgccgg gcaaccagcg
     3241 cagtcctgcc aggtagagcg tcgcgacgcc gacgcccacc ggcagccaga tggcggcgac
     3301 cggcccggtg tactgcagcg cgaggctggc ccgccccgcg agaaagtagg cggccgcgag
     3361 cgccagcacg ccggcggcgt accgcgccga ccagactgcc acttgggagc cctgccgggg
     3421 cgccgccggt gcagggccga gcatcaggcg cgagctcgta gaacctcctt tcgtaaggac
     3481 gctaccgtcc cgcgcggccc gagtcgagcg atgccgtcgg ggcagatcac gcgcggaacg
     3541 cgcgcgtctg ggccaacggg cgtgtcacac gttcaagtac gcgggctgcg aacggcatgt
     3601 gtcgcgagcg ccacacgtgt cgctcctcga agggccaggc atagatcggg ttgccgtcgc
     3661 gaacgagccc gagcacgcgc gggtcggata gctcatcggc ggaccattcg tataggtgct
     3721 gcaggcgcgg cacgatgact gcgtagtcga gcatccggcc tagacgctgt tcgccctcga
     3781 tatagcgctc gacgtcgagg gtgagcggat agcggtccgg gagcacgcgc cggagtgaga
     3841 gaaatactcc cgccatcccg aggcgcggat caccgaccag gcggccgaca ggagccaagc
     3901 ctcccagcgc cagccgtggc gccgcattca gtgcgtgcgc gtacagcaca cgcgccagcg
     3961 cgacgttcat gaaaaagcgc tccggtgagc tttcggcttc ggcgagtttc cggtgctcca
     4021 ggtaggcgcc cacgacgctc gcgttatggg cgcggtacca attgcgcccc gtcggcctct
     4081 cgccgaactc cagccagagt cgcacggcct gagacgacgg ctcgccggcc gacccgccca
     4141 gcagcgcgac ggtttcgcat ccgtcccgca gcagccgctc gttgaccgcc cgccaccaca
     4201 cgctgcccgg cggcgacgcg ccgagcgggt tgagcacacc tcggcgcgcc tgccagctca
     4261 tgaacgacag cgctgcccgt cggaacggta ggtgtcgcgg tgcgtggccc gtcggcccgt
     4321 gaaagacccg ccccatcaat gcaaggcgcg ccgccggatc gtcgcgtacg gccgcgacct
     4381 gcgcctcagc ctgggcgagc gcgcttggga ggtcgctcat cgccgagtag tgattgccgt
     4441 ccggcgactc tacagcgcac accagggagc gggccaggct aagcgacgac ctccatgccg
     4501 accttctcct cgtcggaggc gtcggcgggg ttctcgaccg cgagcacggc ctcgagggca
     4561 gcgatcgcca cggcgagctg cgagaggtcg ggctcgcgtg tcgtgagctt ctgaagctgc
     4621 atcccgggcc acatcagcgc ccgcgcccag cgcttctgcc ggttcttgcc gaagagcttg
     4681 atgatctcga acgccaggcc tgcgaccagc gggatgccga tcacgcgcga cgcgaacagc
     4741 cagtaccaga ccggcgtgcc gagcggagcg aacacgaaga aggccacgat catcacgatc
     4801 agcaggaagc tcgtgccgca gcgcgggtgc aggcgggaga agcgcttcgc gttctgcggg
     4861 gtgagcggca ggccggcctc gaagcacgag atcgtcttgt gctcggcgcc gtggtactcg
     4921 aacacgcgct gcaggtcggc taggcgcgag atcagccaga ggtagagcac gaagatcgag
     4981 atgcggatga gcttctcgat cagcacgaac acgaacgagt tcggcagcgc gtccttgaac
     5041 aggttggtga cgcccgcggg caggatgaag aacagcccga tcgcgaacag caccgaaaac
     5101 agcacggtgc cgaaccaggc gccgcggccg acctcctcct cgccctccgg cacctgcgcg
     5161 ttggccgaga tgccgagggc cttgaagccg atgcccagcg actcgacgag cgcgacgacg
     5221 ccgcggacga tcggcaggcg cagcagccgg tgcttcttga gcaccgaggt cagcgggaac
     5281 tcctcgaccg cgatctcgcc gaggacgacg tcgtctggcg cgtcctcggg cacgggcttg
     5341 cgcacggcga cggcccagtg gccgacgccg cgcatcatca cgccctccag gacggcctgg
     5401 ccgccgaccg gggcgtcgcg ctgggcttgg agctggtcgc cctcgggcgc gacgcgggcc
     5461 ggggcggcgc tcatgtcgct gctacttcga ggaggccttg gccgcgcggc ggcggaagcg
     5521 atcgacgcga ccgcccgtgt ccacgagctt ctggcgaccc gtgtagaacg ggtggcagtt
     5581 cgagcagatc tcg
//
LOCUS       DASVXS010000237        17797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_561739, whole genome shotgun sequence.
ACCESSION   DASVXS010000237 DASVXS010000000
VERSION     DASVXS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 17797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17797
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561739"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VF752_13410"
     CDS             <1..266
                     /locus_tag="VF752_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856588.1"
                     /translation="WREWFAPEFEVDSDRPIPAGIDGEALVLDPPLHFRILPGVLRVR
                     IARQHPGASPSALAPEGIRAGTVELIRIARGQEDPARPTTKEA"
     gene            267..485
                     /locus_tag="VF752_13415"
     CDS             267..485
                     /locus_tag="VF752_13415"
                     /inference="COORDINATES: protein motif:HMM:NF040111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HEX8856589.1"
                     /translation="MDLMEVTERERLRREDAAARLHVLADALARHNEIEFERGGVTFK
                     VNVPDEVDFKLEIEIEEDERELEVELKW"
     gene            complement(562..1560)
                     /locus_tag="VF752_13420"
     CDS             complement(562..1560)
                     /locus_tag="VF752_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8856590.1"
                     /translation="MSRKSTRGLRVLLAAGLSLAVAVGAGCGGGNNNNSTSGGGGSAQ
                     GKKLTLIAGVKGDEFYITMNCGAQAEAKKEGVSLNFQGPDQFDASLQTPIVNAVAAKK
                     PDAILIAPTDTKAMYAPIKQAESNGSKIVLVDTTLDNASVAVSQIASDNLGGGKTAAD
                     TLAKLIGGKGKVFVNNVKPGISTTDLRAKGFQEGAKAAGLQYIGQQYNQDDPAKAASI
                     TKAIIAKNPDLKGIFATNLFGAEGTASALKEAGLLGKVKIVGFDAGPKQVRDLKEGLV
                     QALIAQRPAQIGQLGVQQAVAALTDKPVKSKIATGFVSITKDNLSQNQNALYKASC"
     gene            complement(1596..2636)
                     /locus_tag="VF752_13425"
     CDS             complement(1596..2636)
                     /locus_tag="VF752_13425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8856591.1"
                     /translation="MSETSTAPQQEPAILEEEPRERVPWWQRFVTGSSTWIGLILVGL
                     IVVFSALEYTSFVSSSNARNIATDAAVLLVLAVGMTYVIITAGIDLSVGAVLVFSGVV
                     AAKLMNSVGGNNWGTILIGLVASLAAGLAWGTLNGFLVAKAKIPALIVTLGTLGMALG
                     AALLITGGVDEREVPTKLIDTIGTGRVFNQVPYLVIIAFSVAIVFGIVLAMTRFGRNT
                     YAIGSNEEAARRAGIAVDRHLIKVYALSGVLAGLAGFMNVARFATTTIGGHNTDNLQA
                     IAAVVIGGTSLFGGIGTVLGSVFGVFIPAVLQNGFVIIGVQPFWQQVAVGAVLIGAVY
                     LDQLRRRSVQNR"
     gene            complement(2633..3394)
                     /locus_tag="VF752_13430"
     CDS             complement(2633..3394)
                     /locus_tag="VF752_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8856592.1"
                     /translation="MAEPLLEARGIVKNFGRVHALRGANFTVNSGEVVALVGDNGAGK
                     STLVKTLAGVLAPDAGEILFEGRPVAIHTPLEARALGIETVYQDLALAADLDPAANLF
                     MGREVLRGGVLGKLGFLDKREMHRRADEAFKTLGIGLQDSSAPVANMSGGQRQGVAVS
                     RAVTWASKVVFMDEPTAALGVVQTRHVLDLIRRVREQGLSVVLISHNMPEVFEVADRI
                     EVLRLGERVAQFKTSDTTMEAVVGAMTGAIAQEAA"
     gene            complement(3401..4756)
                     /locus_tag="VF752_13435"
     CDS             complement(3401..4756)
                     /locus_tag="VF752_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX8856593.1"
                     /translation="MRDLLLGIDVGTSVSKAAVVDADGRELAHGEAPTPWQRVETGAE
                     TDPQVLFDAALAAARGALANAPEGRVRGVGVTSMAEAGVLLDARGEVLAPTIVWHDTR
                     GEEEAARIAADFGPERFAERTGLPATALCTLAKLRWLVHHRPETRAGARWFNVAEWVA
                     HRLGARHVSELSLTSRTGLLDLSTKAPFHEAFEWVGLPEELQGELVLAGTPIGTVDVS
                     ALPEAAGAALTVGGHDHLCAGVGAGVVDPGDVLDSSGTAEALVRVAAPLDADQVRRSV
                     AGGVTVGWHAVEGRHALLGAVWSGLALTEVLGALGVTDAERDALDSAAMALPPSDVPE
                     IPLDLYSFERPPLELPEDLPAAAIWRGAIESAVSGVERVLAHIEDIAGPRGRIVSTGG
                     WSRERAVMAAKDRIGRFETPAVVQAGARGAALLGGVAAGLYESADTLPPIPTLAAAER
                     S"
     gene            complement(4753..5766)
                     /locus_tag="VF752_13440"
     CDS             complement(4753..5766)
                     /locus_tag="VF752_13440"
                     /inference="COORDINATES: protein motif:HMM:NF044836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX8856594.1"
                     /translation="MRPVVLPPNGVPRFYRGGAAIAALRGSEPEGDNMPEDWVGSMTT
                     VFGEKQLGLSRLPDGTLLRDAAGADPVGFFGPEHAATRGADPGLLVKLLDAGQRLPVH
                     VHPDDSFARAELGTPYGKTEAWLVIGAAPGANVGVGFSEDIDPETLERWVREQDTEAM
                     LGSLNRVDVRPGDAIFVPAGVAHAIGEGVLIVELQEPTDLSVLLEWRGFADGDGDGDE
                     ATLGLGWDTALRCVQLTRSDPSELVNGGDSGHSLLPRTADGFFRAERLDPDAAAEIEQ
                     GFAILVVTEGSGTLACESGEPLPVRRGDSVLLPWSAGDCRLDGELVALACRPPAPAQE
                     AAA"
     gene            complement(5763..6767)
                     /locus_tag="VF752_13445"
     CDS             complement(5763..6767)
                     /locus_tag="VF752_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8856595.1"
                     /translation="MARPTMRDVASTAGVSVKTVSRVVNDEPGVRPDTAARVDAAIAQ
                     LGFARNDLARGLRRGRAGALGLIIEDVANPFYSAVARAVEDVAHERGHILITGSCEED
                     PSRERQLALGLLRRLVDALLIVPAGSDHRYLMPDLRAETPIVFLDRPPEGLPADSVLV
                     DNHGGARAAVEHLVQRGHERIGFVGDPPALYTARVRLDGYRTALADAGIAVREELIRA
                     GSHDAVAAEQSGRELLALPESDRPTALFSSNNRNTIGVLRALRDAPRPVALVGFDDFE
                     LADMLATPVTVVRNAPEEMGRIAAELAYARLDGDGARQPVERIVPCELVPRGSGEIAP
                     "
     gene            complement(6874..7836)
                     /locus_tag="VF752_13450"
     CDS             complement(6874..7836)
                     /locus_tag="VF752_13450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEX8856596.1"
                     /translation="MLIAGPNLTIDRTLTIDELLPGHVLRFENAAITPGGKGVNVARV
                     ARELGAPALLVGFTPGHTGRAVARLIADEGLKLRPVEVAGELRAAIVVLERGGRVTVL
                     NEPGPTLGEGDWDRYEAVVAEGFAEHSVMVCSGSLPPGTPRDAYARLAVLAERAGVTA
                     IVDGAGDVLGAALDANPDVVTPNLAEAEGLLHGRADESVEAGDPVAVRDRAGTAAAEL
                     VSRGARAAVVTAGGAGAALANRSNVIWHPAPNVTVHNPIGAGDSFAGGLAAALERGED
                     LGAAVAFGLATAAASVESATAGVIDPERAAELASSAPPTETLLN"
     gene            7869..8705
                     /locus_tag="VF752_13455"
     CDS             7869..8705
                     /locus_tag="VF752_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856597.1"
                     /translation="MSSHYRLNRLFDARSGRCIDVAVDHGFFGEPPFLAGIEDMGAAI
                     DTLVAAGPDAIQLTVGQAPLLQSKPGREKPALVLRVDVANIYGPEPPSEPFSSVVDDA
                     VGHAVRLDAACVCVNLLDIPGMDGLRRQCVENIAALRTACDRTGIPLMVEPLVMKPGA
                     SGYDSDGDLDRILALVRQATELGADLIKADPADDLSQYHRVITVARVPVLVRGGGRVP
                     DDELLRRTHAVLEQGAAGIVYGRNIIQHASPAGMVAALRAVVHEGVSPEVAAESLAGA
                     PR"
     gene            8702..9829
                     /locus_tag="VF752_13460"
     CDS             8702..9829
                     /locus_tag="VF752_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8856598.1"
                     /translation="MTTERVRVGIIGGGLMGRELAGAIGRWAVLEQHPIWPELVAVCD
                     TNPEALAWFDRIESVRLRAADYTELLADDEIDVLYIAVAHHLHERLYLDAIAAGKDFL
                     AEKPFGIDLDAATRIVAALEASPELFVRCSSEFPFFPGAQLAFEAIRSGALGELVEVE
                     NAFLHSSDLDRSKPINWKRQARYCGAIGVMGDLGMHVTHVPLRLGWRPRSVFAELQDI
                     VKERPGPDGELVRCDTIDNASLYCDAGFPLTLRTYRIAPGNMNTWRLSAIGMDGGVVF
                     STAEPKTVRRFAIRDGRQVWEQLEVGSQSSFPTITGGIFEFGFADAILQMWASFLAER
                     AGALGDRFGCATPHEAFESHRIFAAALRSGETASAEPVPGA"
     gene            9932..10180
                     /locus_tag="VF752_13465"
     CDS             9932..10180
                     /locus_tag="VF752_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856599.1"
                     /translation="MAHRKNIIARAIAHQRSDSGRPTEPGDALSEEGTTLDALLARLD
                     RLEAQLEGLQDAVYREALRQNGRITAISKSRHNAAGHQ"
     gene            10177..10521
                     /locus_tag="VF752_13470"
     CDS             10177..10521
                     /locus_tag="VF752_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856600.1"
                     /translation="MKAVEPAFFVPGHDDEPAEAEAAYTTIRKTITVSTGHVPRDRRI
                     WRLSARRGGRDCITQVGLPDPLSSELVVAILDLGTHKPFAVYSRCSHGGDEIEELQLQ
                     QVYEVVEFPPLL"
     gene            10633..10836
                     /locus_tag="VF752_13475"
     CDS             10633..10836
                     /locus_tag="VF752_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8856601.1"
                     /translation="MPTGTVKWFSDEKGFGFVTPDEPGADLFVHHTGIVGDGYKSLAE
                     NSKVQYDSEPGDKGPKAVNVQLI"
     gene            10903..11931
                     /locus_tag="VF752_13480"
     CDS             10903..11931
                     /locus_tag="VF752_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8856602.1"
                     /translation="MEIARRVAAFIEPARETLDLALYDVRLPSPIAEVVKGAITAAAQ
                     RGVQVRLAYNLDNERHPKPLPPPPRTEPTLLETLGVPMCGIPGEPDLMHHKYVIRDGQ
                     SVWTGSTNWTADSWSREENVVLTVDSPELAANYTRNFEELWSTREVDKSGREEPAAVD
                     VGGVRVSAWFCPGKGEDLSHRIAAALGRARRVRIASPVITSAPVLATLAQIACDGRCD
                     VAGVVDATQIAQVRQQWHANGNARWKLPLLATVFERSAFTGKISTPYRPGSVHDYMHA
                     KVTVADDIVFAGSFNLSHSGEQNAENVLEIRDAGLAERLSAFIDQVRSRYRPVPPESG
                     DYTRDLND"
     gene            complement(11928..12596)
                     /gene="nucS"
                     /locus_tag="VF752_13485"
     CDS             complement(11928..12596)
                     /gene="nucS"
                     /locus_tag="VF752_13485"
                     /inference="COORDINATES: protein motif:HMM:NF002876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEX8856603.1"
                     /translation="MRLIVARCEVRYSGRLSAVLPEALRLLLIKSDGSVMVHSDAGGY
                     KPENWMTPPCVVEETGEGLEVRKVGADDRLEIRFAEVVSDVTHDMGEAAALQKDGVER
                     HLQELLAESPEWCGEGFRLVKREWATDIGPVDLMCRDSADCWVAVEIKRIGTIQAVEQ
                     LTRYLERIRQEPGMSRCRGVLAAQVVKPQARVLAESRGIGWVEVDLDVLRGEREPDLT
                     LFAA"
     gene            12677..13372
                     /locus_tag="VF752_13490"
     CDS             12677..13372
                     /locus_tag="VF752_13490"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX8856604.1"
                     /translation="MPLQLLVLALDAALYPTLLAAVVVLLSQPRRRPLLTAYLAGGLT
                     ISIGLGLGIVAALKDSKAVDSSHSGLSWVMDLAVGGLAMLLAVALATRADVRFRERRR
                     STRPAKPVDADEEKKEPLSQRVLARGSVPTVLIAALALNLPGAAYLVALKDIAAAHHP
                     TSVDIALVVGFNLIMFLLAEIPLLGLIFAPEQTVARVKRVNAWFLANSRTIAVVLSAV
                     LGAFLITRGIVNS"
     gene            complement(13736..14842)
                     /locus_tag="VF752_13495"
     CDS             complement(13736..14842)
                     /locus_tag="VF752_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8856605.1"
                     /translation="MRIRAAVLEEFGAPLEVQELDLAEPKAGEVLVRLEACGVCHTDL
                     YTASGADPSGYAPTVLGHEGAGVVEKIGDGVTSLSPGDHVVTLFSPQCRECVHCLSDR
                     TNLCLAIREQQNSGFLPDGTARLSRNGEPVRHFMGTSTFAEYTVMPEIALARVNPDAP
                     VDRACLFACGLSTGLGAAINTAEVEPGSTCIVFGAGMVGLGAVAGCRLQGADRIVCVD
                     LSDDRLELARGQGATDVMQGGPDVVERILEMTGGFGADYTFEATGNVAVMRQAVQAAR
                     MGWGLCTVAGVAGKGETLDIVPRLLITGRRVAGSSFGGVKGRDQVPEFVESYLAGDID
                     VDPFVSHRIRLDDVNRGFELMEAQDGIRSVIEFA"
     gene            14921..15532
                     /locus_tag="VF752_13500"
     CDS             14921..15532
                     /locus_tag="VF752_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856606.1"
                     /translation="MSGLPITEAMTPAEATHESAPSEFVLYEEYLGVYLAMRPGDGGW
                     LIETRKTPDGEAVASFKRPLVHVLEWLTSEKAAWALSSHRDKLEAVRAAAKELLRSLP
                     PGSFIGAEEVGAIELAADRMQVLRRDLDEVVDHEGRARRRRRVEEMVASGEIGATPSS
                     RTKSASRASRDGATAGRCSAISSRRGGGCAAPSSQSRSAKHSS"
     gene            15541..15747
                     /locus_tag="VF752_13505"
     CDS             15541..15747
                     /locus_tag="VF752_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856607.1"
                     /translation="MERQAAIARHHRALELARRAALKTLEDVPLGDWPEVLETAAAMY
                     TFTGLAREIEEAVEVRSAADVPLY"
     gene            complement(15754..17130)
                     /locus_tag="VF752_13510"
     CDS             complement(15754..17130)
                     /locus_tag="VF752_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8856608.1"
                     /translation="MRRILLVLVALALLGLAPAAAHAHPLGNFSINHLDQVRVSADQV
                     QVRYILDQAEIPTFQERGLAPAEVLARKRTEVMRGVELTVDGRRLPLELQPGASISFP
                     PGQGGLKLTRVEIPLSARVAGARSVHIHDGTFEGRVGWKAIVIQPGWGTAVTSSVPAA
                     DPTGGLRAYPKDTLSSPLDQRSARFSAKPGHGTVSAPPSPFASAQVTHDRSGDGFTKV
                     LDDAIAGHGVLVFLLLAAFAWGAVHALSPGHGKAMVAAYLVGARGRPRHAVALGATVT
                     VTHTIGVFALGVVTLLLSQFIVPEDLYPWLNLASGLLVVSIGAAVLRSRLRARSRAHP
                     HRHHDHDHHHDHDHHHPPDEISWKGLLGMGASAGLIPCPSALVVLLGAISQHEVALGL
                     LLILAFSFGLAGTLTALGLAVVYARRALTRVSFFGGGVVKALPAVSALVIVCAGAVLT
                     AQAVPRLV"
     gene            complement(17127..>17797)
                     /locus_tag="VF752_13515"
     CDS             complement(17127..>17797)
                     /locus_tag="VF752_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8856609.1"
                     /translation="ENVAYVQTLLGNLLFDTGRLGAAREAYRTALARFPGYVPASAGL
                     ARVEAARGDLGAAIRRYSSAVARLPLPEYVVGLGETEQAAGRRAQARHDLALVRAEER
                     LLQANGVNTDVDLALFEASHGSPKRAVMLARRAWSEASSVRSADALGWAYTRAGRPGA
                     GLRWAQRALRLGSRDPMFLFHAGMAARAAGRLDCARAYLARSLELNPRFNPLYARQAQ
                     EALR"
BASE COUNT         2713 a         6325 c         6139 g         2620 t
ORIGIN      
        1 cctggcgcga gtggttcgcc cccgagttcg aggtcgattc cgaccgcccc atcccggccg
       61 gcatcgacgg cgaggcgctg gtgctcgacc cgccgctgca cttccgcatc ctgccgggcg
      121 tgctccgcgt gcggatagcg cgccagcatc cgggtgcgtc gccgtcagcg ctggcgcccg
      181 agggaatccg cgccggcacg gtcgagctga tccgcatcgc ccgaggacag gaagaccctg
      241 caaggccaac gaccaaggag gcatgaatgg acctgatgga ggtcaccgag agagagcgcc
      301 tacggcgcga ggacgcggcc gcgcgcctgc acgtgctcgc tgatgcgctc gcgcgtcaca
      361 acgagatcga gttcgagcgc gggggagtga cgttcaaggt caacgtccct gacgaggtgg
      421 acttcaagct cgagatcgag atcgaggagg acgaaaggga gctcgaagtc gagctcaagt
      481 ggtgacctga ggaacgcccg gcggcgctcg gcgagtccgc cgccctgggg tctcgccgag
      541 cgcatgctgc cggggtctgt ctcagcagct cgccttgtag agcgcgttct ggttctgaga
      601 caggttgtcc ttcgtgatgg acacgaagcc cgttgcgatc ttcgacttca ccggcttatc
      661 cgtgagcgcc gcgacggcct gctggacacc cagctggccg atctgcgccg gacgctgagc
      721 gatcagcgcc tgcacgagcc cttccttcag gtcccgcacc tgcttgggac ctgcgtcgaa
      781 gccgacgatc ttgaccttcc cgagcaggcc ggcttccttg agcgcgctcg cggttccctc
      841 cgcgccgaat aggttcgtcg cgaagatgcc cttcaggtcc gggttcttgg cgatgatcgc
      901 cttggtgatc gatgcagctt tggccgggtc gtcctggttg tactgctggc cgatgtactg
      961 cagtccggcc gccttggcgc cctcctggaa gcccttggca cggaggtccg tggtcgagat
     1021 gccgggcttc acgttgttca cgaacacctt gcccttgcca ccgatcagct tggcgagggt
     1081 gtcagcggcc gtcttcccgc cgccgaggtt gtcggaggcg atctgcgaca ccgcgacgct
     1141 cgcgttgtcg agcgtggtgt ccacgagcac gatcttcgag ccgttcgact cggcctgctt
     1201 gatcggcgcg tacatcgcct tcgtgtcggt cggcgcgatc aggatcgcgt ccggcttctt
     1261 ggccgcgacc gcgttcacga tcggggtctg aagcgaggcg tcgaactggt cgggcccctg
     1321 gaagttcagg ctcacgccct ccttcttggc ctcggcctgc gctccgcagt tcatggtgat
     1381 gtagaactcg tcgcccttca cgccggcgat cagggtgagc ttcttgccct gggcgctgcc
     1441 gccaccgccg ctggtcgagt tgttgttgtt gccgccacca cagcctgctc ccacggcgac
     1501 ggccagggac agcccggccg cgaggagcac gcgtaggcca cgcgtggatt tgcgtgacat
     1561 ctggtccctc tccctcctga ttgagttgat ctcgttcatc ggttttgcac tgagcgtcgg
     1621 cggagctggt cgaggtagac ggcgccgatc agcaccgcgc cgaccgcgac ctgctgccag
     1681 aacggctgaa cgccgatgat cacgaacccg ttctgcagga ccgccgggat gaagacgccg
     1741 aacacggacc caaggaccgt gccgatgccg ccgaacaggc tggtaccgcc gattacgacg
     1801 gcggcaatgg cctgaaggtt gtccgtgttg tgacctccga tcgtcgttgt cgcgaagcgc
     1861 gcgacgttca tgaatccagc caggcctgcg agcactcctg agagcgcgta gaccttgatg
     1921 aggtgccggt cgaccgcgat gccggcacgg cgtgccgcct cctcgttcga cccgatcgcg
     1981 taggtgttgc gcccgaagcg ggtcatcgcg agcacgatcc cgaacacgat cgccacgctg
     2041 aacgcgatga tcaccaggta cggcacctgg ttgaacacgc gtccggtgcc gatcgtgtcg
     2101 atcagcttgg tgggtacctc ccgctcgtca accccgcccg taatgagcag cgccgcgccg
     2161 agcgccatgc cgagcgtgcc gagcgtgacg atgagcgccg ggatcttggc cttggccacg
     2221 aggaagccgt tcagcgtccc ccatgcgagc ccggcggcga gcgaggcaac gagcccgata
     2281 agaatcgtgc cccagttgtt gccgcccacc gagttcatca gcttggcggc caccacgccg
     2341 ctgaacacga gtaccgcgcc cactgacagg tcgatgcccg cggtgatgat cacgtaggtc
     2401 atgccgacgg cgagcacgag cagcacagct gcgtcggtcg cgatgttgcg ggcgttcgac
     2461 gacgagacga agctcgtgta ctcgagcgcg gagaagacga cgatcaggcc gaccaggatg
     2521 aggccgatcc aagtgctcga cccggtcacg aagcgctgcc accagggcac ccgctcgcgc
     2581 ggctcctctt cgaggatggc gggctcttgt tgcggtgctg tgctggtctc gctcatgccg
     2641 cctcctgtgc gatcgccccg gtcatggctc ccaccaccgc ttccatggtc gtgtcggagg
     2701 tcttgaactg agcgacgcgc tcgccgagcc gcagcacctc gatgcggtcc gccacctcga
     2761 acacctcggg catgttgtgg ctgatcagga cgaccgacag cccctgctcg cgcacgcgcc
     2821 ggatgaggtc gagcacgtgc cgtgtctgca ccacgccgag tgcggccgtc ggctcgtcca
     2881 tgaacacgac cttgctggcc cacgtgaccg cgcggctcac cgccacgccc tgccgctggc
     2941 cacccgacat gttcgcgacg ggcgccgagg agtcctgcag gcctatcccg agcgtcttga
     3001 acgcctcgtc ggcgcgccgg tgcatctcgc gcttgtcgag gaatccgagc ttgccgagca
     3061 cgccgccgcg cagcacctcg cgtcccatga acaggtttgc ggctggatcg agatcggccg
     3121 cgagggcgag atcctggtag acggtttcga ttccgagcgc gcgcgcctcg agaggtgtgt
     3181 ggatcgcgac ggggcggccc tcgaagagga tctcgcccgc gtccggagcc agcacgccgg
     3241 cgagtgtctt cacgagcgtg ctcttgcctg cgccgttgtc tcccacgagc gcgacgacct
     3301 cgccggaatt gacggtgaag ttcgcgccgc ggagggcgtg aactcgaccg aagttcttca
     3361 ctatgccgcg cgcctcgagt agaggctcag ccattggtgc tcaactcctt tcggccgcag
     3421 ccagggttgg gatcgggggt agggtgtcgg cgctctcgta caggccggcg gcgacgccgc
     3481 cgaggagcgc cgcgccgcgc gctccggcct ggaccacggc cggcgtctcg aagcgcccga
     3541 tgcggtcctt cgccgccatc accgcgcgct cgcgcgacca tccgccggtc gacacgatgc
     3601 gcccgcgggg gcctgcgatg tcctcgatgt gtgcgagcac gcgctccacg ccggagacgg
     3661 ccgattcgat cgcgccgcgc cagatcgcgg ccgcgggcag gtcttccggc aactcgagag
     3721 gcgggcgctc gaacgagtac aggtcgagcg gaatttctgg cacgtcgctg ggcgggagcg
     3781 ccatcgcagc ggagtcgagt gcgtcgcgct ccgcgtccgt tactccgagc gcgccgagca
     3841 cctcggtaag cgcgaggccg ctccacactg cgccgagcag cgcatggcgc ccctcgaccg
     3901 cgtgccagcc cacggtcacg ccgccggcca cggagcggcg cacctgatcg gcgtcgagcg
     3961 gagccgccac gcgcacgagc gcctcggccg tgccgctcga gtcgagcacg tcgccggggt
     4021 ccacgacgcc cgcgccgacg ccagcgcaca gatggtcgtg gccgcccacg gtgagggcag
     4081 cgcccgccgc ctccggcagc gccgaaacgt caaccgtgcc gatcggcgtg cccgcgagca
     4141 ccagctcgcc ctgcagctcc tcgggtagcc cgacccattc gaaggcctcg tggaagggcg
     4201 cctttgtgct cagatcgagc aatccggtgc gcgacgtgag cgacagctcg gacacgtgcc
     4261 tcgcgccgag acggtgagcg acccactcgg ccacgttgaa ccaacgtgcg cctgcccgtg
     4321 tctctgggcg atgatgcacg agccagcgca acttggcgag cgtgcatagc gccgtggccg
     4381 gcaggccagt gcgctcggcg aacctctcgg gcccgaagtc ggccgcaatg cgagccgcct
     4441 cctcctcgcc tcgcgtgtcg tgccagacga tcgtgggcgc gagcacctcg ccgcgggcgt
     4501 cgagcaatac gccagcctcc gccatgctcg tcacgccgac accgcgcacg cgtccctcgg
     4561 gggcgttggc gagcgccccg cgcgcggccg cgagggccgc gtcgaagaga acctgcgggt
     4621 cggtctcggc tcccgtctcc acccgctgcc agggagtcgg cgcctcgccg tgcgcaagtt
     4681 cgcggccgtc ggcgtcgacc accgccgctt tggacacgct cgtgcccacg tcgatcccga
     4741 ggagcaggtc tctcatgccg ccgcctcctg tgcaggtgcg ggcgggcggc aggcgagcgc
     4801 cacgagttcg ccgtctagac ggcagtcccc cgcgctccac ggcagcagca cggaatcgcc
     4861 gcgtcggacc ggaaggggct cgccgctctc gcacgcgagc gtgccactcc cctcggtcac
     4921 gaccaggatc gcgaaccctt gctcgatctc ggcggccgca tcgggatcca gccgctcggc
     4981 acggaagaag ccgtcagccg tgcgcggcag cagcgagtgt cctgaatcac cgccgttcac
     5041 gagctcgctt gggtccgagc gcgtgagctg tacacagcga agcgcggtgt cccagccgag
     5101 gccgagcgtg gcctcatcgc cgtcgccgtc gccgtcggcg aagccgcgcc actcgagcag
     5161 cacggacagg tcggtcggct cctgcagctc gacgatgagc acgccctcgc cgatcgcgtg
     5221 cgccacgccc gccggcacga agatcgcgtc gccgggccgc acgtccactc ggttgagcga
     5281 gccgagcatc gcctcggtgt cctgctcgcg cacccagcgc tcgagcgtct ccggatctat
     5341 gtcctcgctg aagccgacgc cgacgttggc gccgggcgcc gcgccgatca cgagccaggc
     5401 ctcggtcttc ccgtatggcg tgccgagctc cgcgcgtgcg aaggaatcgt ccgggtggac
     5461 gtgcacgggc agcctctgtc cggcgtcgag cagcttcacg agcagcccgg gatccgcgcc
     5521 gcgtgtggcg gcgtgctcgg ggccgaagaa gccgacgggg tcggcgccgg cggcatccct
     5581 caggagcgtg ccgtccggca gccgactgag gccgagctgc ttctcgccga acaccgtggt
     5641 catcgaaccg acccagtcct cgggcatgtt gtccccctcc ggctcgctgc cgcgcagcgc
     5701 ggcgatggcc gcgcctccgc ggtagaagcg cggcacccca ttcggcggca gcacgacggg
     5761 cctcacggcg cgatctcccc ggatccgcgt ggcacgagct cgcacggcac gatgcgctcg
     5821 accggttgcc gcgcgccgtc cccgtcgagc cgggcgtacg cgagctcagc ggcgatgcgg
     5881 cccatctcct cgggcgcgtt gcgcaccacc gtcacaggcg tggcaagcat gtcggcgagc
     5941 tcgaaatcgt cgaagcccac gagcgcgacc gggcgcggcg cgtcgcgcag cgcgcgcagg
     6001 acgccgatcg tgttgcggtt gttcgacgag aagagcgcgg tgggacgatc gctctcgggg
     6061 agagcgagca gctcgcgtcc cgattgttcg gcggcgacgg cgtcgtgcga gccggcccga
     6121 atgagctcct cgcgcactgc aatgccggcg tccgcgagcg cggtgcggta gccgtcgagc
     6181 cgcacgcggg cggtgtacag cgccggcggg tcgccgacga agccgatccg ctcgtggccg
     6241 cgctgcacca ggtgctcgac cgcggcgcgc gcgccgccgt ggttgtccac gagcacgctg
     6301 tcggcaggca gtccctcggg cgggcggtcg aggaagacga tcggcgtctc ggcgcgcagg
     6361 tcaggcatca ggtagcggtg atccgaaccg gccggcacga tcaggagcgc gtcgaccagg
     6421 cgccggagaa gcccgagcgc cagctggcgc tcgcgactcg gatcctcctc gcacgagccc
     6481 gtgatcagga tgtggccgcg ctcgtgtgcg acgtcctcga cggcgcgggc gaccgccgag
     6541 tagaaggggt tcgcgacgtc ctcgatgatc agcccgagcg cgcctgcccg accgcggcgc
     6601 agcccgcgcg cgaggtcgtt ccgcgcgaag ccgagctgcg cgatcgccgc atccacgcgc
     6661 gcggcggtat cgggccgcac gcccggctcg tcgttcacaa cccgcgacac ggtcttcacg
     6721 ctcacacccg cggtgctagc gacgtctcgc atcgtcggcc ttgccaccta tgggacctct
     6781 ccatctccca cgcctgacaa cgatgtccag gcttcctcgt gcggcaccgt agagcgtgcc
     6841 tgacaacgtt gtcaagagcg gaaatgttgt gcttcagttg agcaacgtct cggtcggcgg
     6901 tgcgcttgac gcgagctccg cggcccgctc agggtcgatc acgcccgccg tcgcggactc
     6961 gacgctggcc gcggccgtgg cgagcccgaa ggccaccgcc gcgcccagat cttcgccgcg
     7021 ctcgagcgcc gcggccaggc ctccggcgaa cgagtcgccc gcgccgatcg ggttgtgcac
     7081 ggtcacgttc ggggcgggat gccagatcac gttcgatcga ttggccaggg cggcgccggc
     7141 accgccggcg gtgacgaccg ccgcgcgcgc gccgcggctc acgagctccg ccgccgccgt
     7201 gcccgcgcgg tcgcgcaccg cgaccgggtc gcccgcctcc acgctctcgt cggcgcggcc
     7261 gtgaagcagc ccctcggcct ccgccaggtt cggcgtcacg acgtcagggt tcgcgtcgag
     7321 cgccgcgccc agcacgtcgc ccgcgccgtc cacgatcgcc gtcacaccag cgcgctcggc
     7381 gagcaccgcg aggcgcgcgt aggcgtcgcg cggagtgccg ggcgggaggc tgccgctgca
     7441 caccatgacg ctgtgctcgg cgaagccctc ggccacgacc gcctcgtagc ggtcccagtc
     7501 gccctcgccg agcgtgggtc cgggctcgtt gagaaccgtc acgcgtcctc cgcgctcgag
     7561 cacgacgatc gcggcgcgca gctcgccggc gacctccacg ggccgcagct tcagcccctc
     7621 gtcggcgatg aggcgtgcga ccgcccggcc cgtgtggccc ggggtgaagc ccacgagcag
     7681 cgcgggggcg ccgagctcgc gggccacccg cgcgacgtta acgcccttgc cgcccggcgt
     7741 gatggcggcg ttctcgaacc gcagcacatg gccgggtagc agctcgtcga tcgtcagagt
     7801 gcgatcgatg gtgaggttgg gacccgcaat cagcattggg acagcatcgt gcttatgatc
     7861 tgacaacgtt gtcaagccac taccgtctga accgtctgtt cgacgcgcgc tcgggccgct
     7921 gcatcgacgt ggccgtcgac cacggcttct tcggtgagcc gccgttcctc gccgggatcg
     7981 aggacatggg tgccgcgatc gacacgctcg tcgcggcagg cccggatgcc atccagctca
     8041 cggtcggcca ggcgccgctg ctgcagtcga agcctggtcg cgagaagccg gcgctcgtgc
     8101 tgcgcgtgga cgtggccaac atctacggcc cggagccgcc gtcggagccg ttcagctccg
     8161 tggtcgacga cgcggtcggc cacgcggtgc gcctcgatgc cgcctgcgtc tgcgtgaacc
     8221 tcctggacat ccccggcatg gacggcctgc ggcgccagtg cgtcgagaac atcgcggcgc
     8281 tgcggacagc atgcgaccgc accggcatac cgctcatggt ggagccgctc gtgatgaagc
     8341 cgggcgcgag cggttatgac tctgacggcg acctcgaccg catcctcgcg ctcgtgcggc
     8401 aagcgaccga gctcggtgcc gatctgatca aggccgatcc ggccgacgac ctctcgcagt
     8461 accaccgcgt aatcactgtc gcgcgcgtgc cggtgctcgt acgcggcggt ggccgcgtcc
     8521 ccgacgacga gctgcttcgg cgcacacacg cggtgctcga gcagggggcc gcgggcatcg
     8581 tgtacggccg caacatcatc cagcacgcca gcccggccgg catggtcgct gcgctgcgcg
     8641 ccgtcgtgca cgagggcgtc tcgccggagg ttgctgccga gtcgctcgcc ggagcgccgc
     8701 ggtgacgacg gagcgagtcc gcgtcggcat catcggcggc gggctcatgg gccgggagct
     8761 ggcgggtgcg atcgggcgct gggccgtgct cgagcagcat cccatttggc ccgagctcgt
     8821 ggccgtgtgt gacacgaacc ccgaggctct cgcctggttc gatcgcatcg agagcgttcg
     8881 gctgcgcgcc gccgactaca ccgagctgct cgccgacgat gagatcgacg tgctctacat
     8941 cgcggtggcg caccatctgc acgagcggct gtacctcgac gcgatcgcgg cgggcaagga
     9001 cttcctcgcg gagaagccgt tcggcatcga cctcgacgcg gccacgcgga tcgtggcggc
     9061 gctcgaggcg agcccggagc tgttcgtgcg ctgctcgagc gagtttccct tcttcccggg
     9121 tgcgcagctc gcgttcgagg cgatccgctc gggcgcgctc ggcgagctgg tagaggtcga
     9181 gaacgcgttc ttgcactcga gtgacctgga tcgcagcaag ccgatcaact ggaagcgcca
     9241 ggcgcgctat tgcggcgcga tcggcgtgat gggcgatctc ggcatgcacg tgacgcacgt
     9301 accgctccgg ctgggatggc ggccccgatc cgtcttcgct gagctgcagg acatcgtgaa
     9361 ggagcgcccc ggcccggacg gcgagttggt ccgttgcgac acgatcgaca acgcgtcgct
     9421 ctactgcgac gccgggtttc cgctcacgct ccgcacctac cgaatcgcgc cggggaacat
     9481 gaacacctgg cgcctcagtg ccatcggcat ggacggcggc gttgtcttct ccacggccga
     9541 gccgaagacg gtgcggcgct tcgcgatccg cgacggccgt caggtatggg agcagctcga
     9601 ggtcggcagc cagtcgtcgt ttccgacgat caccggcggc atcttcgagt tcggcttcgc
     9661 cgacgcaatc ctccagatgt gggcgtcgtt cctcgcggag cgtgcgggcg cgctcggcga
     9721 tcgcttcggc tgcgccacgc cgcacgaggc gttcgagagc caccgcatct tcgccgcggc
     9781 gctgcgttcg ggcgagacgg cctccgcgga gccagtgccc ggcgcctgac ctggcgtcag
     9841 gcgcgagttt gcggcctaca ctcgtagaaa gcgccggcag accggacacc ctcaccgccg
     9901 cgcttcccaa cgagggagga aagggtcgga catggcccac agaaagaaca tcatcgcgcg
     9961 ggcgatcgcg caccagcgat cggattccgg gcgccccaca gagccgggcg acgcgctctc
    10021 cgaggagggc acaacacttg acgctctgct cgcgcggctg gaccggctcg aggcgcagct
    10081 ggaaggcctg caggacgcgg tgtaccgcga ggccttgcgc cagaacggac gcataacggc
    10141 aatctccaag agtcgccaca acgcggcggg ccatcaatga aggcggtcga gccggcgttc
    10201 ttcgtacccg gacacgacga cgagccggca gaggccgagg ccgcgtacac gacgatccgc
    10261 aaaacaatca ctgtgagcac cggccacgtt ccgcgcgacc gacgcatctg gcggctctcg
    10321 gcgcgacgcg ggggacgcga ttgcatcacg caggtcggcc ttccagatcc actctcgagc
    10381 gagctcgtcg tcgcgatact cgacctcggg acgcacaagc cgttcgcggt gtactcgcgc
    10441 tgctctcacg gaggcgacga gatcgaggag ctccagctcc agcaggtgta cgaggtcgtc
    10501 gagttcccac ccttgctgta gcgttgacag ccacggttgg gcacaccgcc cgacctcaga
    10561 tgtgcacagc gcccgttggt tgtcgccaca gagcttcttc ccgcgtcgcc gcaagagcac
    10621 aggaggaagc acatgccaac aggcacagtc aagtggttca gcgacgagaa gggctttggc
    10681 ttcgtcaccc ctgacgagcc cggagccgac ctgttcgtcc atcacaccgg catcgtcggc
    10741 gacgggtaca agtcgctcgc cgagaactcc aaggtgcagt acgacagcga gcccggggac
    10801 aagggtccca aggccgtcaa cgttcagctg atctaagcct ttccgggtct agccctggcc
    10861 gccattgacg tcaccaccct gaccgacggc ggccaggacc cgatggagat cgctcgccgg
    10921 gtcgccgcgt tcatcgagcc cgcgcgcgag acgctcgacc tcgcgctcta cgacgtgcgc
    10981 ctgccgtctc cgatcgccga ggtggtgaag ggcgcgatca ccgccgccgc gcagcgcggc
    11041 gtccaagtgc ggctcgcgta caacctcgac aacgagcggc atcccaagcc gctgccacct
    11101 ccgccgcgca cggagccgac gctcctcgag acgctcggcg tgccgatgtg cggcattccc
    11161 ggcgagcccg acctgatgca ccacaagtat gtgatccgcg acgggcagag cgtctggacg
    11221 ggatcgacaa actggacggc ggactcgtgg tcgcgcgagg agaatgtggt gctcaccgtg
    11281 gactcgcctg agctggcggc gaattacacg cgcaatttcg aggagctctg gagcacgcgc
    11341 gaggtggaca agagcgggcg cgaggagccg gccgcagttg acgtcggcgg cgttcgcgtg
    11401 agcgcctggt tctgccctgg aaagggggag gacctctcgc accggatcgc cgccgcgctc
    11461 gggcgggcgc gccgcgtgcg catcgcatcg cccgtgatca cgtcggcgcc cgtgctggcg
    11521 acgctggctc agatcgcctg cgacgggcgc tgcgacgtgg cgggcgtggt cgacgcgacg
    11581 cagatcgccc aagtgcgcca gcagtggcac gccaacggca acgcccgctg gaagctgccg
    11641 ctgctcgcga cggtgttcga gcgcagcgcc ttcaccggca agatctcgac gccctaccgg
    11701 ccgggcagcg ttcacgacta catgcacgcg aaggtcacgg tcgccgacga catcgtcttc
    11761 gccggcagct tcaacctctc gcactccggg gagcagaacg ccgagaacgt gctcgagatc
    11821 cgcgacgccg ggcttgcgga gcggctcagc gccttcatcg accaagtgcg ttcccgctat
    11881 cggccggtgc cgcccgaaag cggggactac acgcgcgacc tgaacgacta ggccgcgaaa
    11941 agagtgaggt ccggctctcg ctcgccgcgg agcacgtcga ggtccacctc gacccagccg
    12001 atgccgcgcg actcggccag cacgcgtgcc tgaggcttca ccacctgcgc cgccaggacg
    12061 ccccggcagc gggacatgcc gggctcctgg cggatgcgct ccaggtaccg cgtgagctgc
    12121 tcgaccgcct ggatggtccc gatgcgcttg atctccacgg ccacccagca gtccgcggag
    12181 tcgcggcaca tgaggtcgac ggggccgatg tcggtggccc attcgcgctt cacgagccga
    12241 aagccctcgc cgcaccactc aggcgactcc gccaagagct cctgaaggtg gcgctccacg
    12301 ccgtccttct ggagcgcggc agcctcgccc atgtcgtgag tcacgtcgga cactacctcg
    12361 gcgaagcgga tctcgagccg atcgtccgcg cccaccttgc gcacctcgag cccctcgccc
    12421 gtctcttcga ccacgcacgg gggcgtcatc caattttccg gcttgtaacc cccggcgtcc
    12481 gagtggacca tcaccgagcc gtccgacttg atgagcagca gccgcagcgc ctcaggcagc
    12541 accgcgctca ggcggcccga gtagcggact tcacagcgag cgacgatcag gcgcatggag
    12601 cgggtaaacg tacggccgcg gccggacaca accttcggaa ggatgcgggc gcgccggatg
    12661 cgctaagacg ttcgccgtgc cgctgcaact tctcgtgctg gcccttgacg cggcgctgta
    12721 cccgacgctc ctcgcggctg ttgtggtcct gctctcacag ccaaggcggc gtccgctgct
    12781 gaccgcctac ctcgccggcg ggctcacgat cagcatcggc ctcggcctcg gaatcgtcgc
    12841 tgcgctgaag gactcgaaag cggtggacag cagccattcg ggcttgagct gggtcatgga
    12901 cctggccgtc ggcgggctgg cgatgctcct cgctgtcgcg ctcgccaccc gggccgatgt
    12961 gcgcttccgg gagcgacgcc ggtccactcg tccggcgaaa cccgtcgacg ccgacgagga
    13021 gaagaaggag ccgctctcgc aacgcgtgct cgcgcgcggc tcggtcccaa cggtgctcat
    13081 tgccgcgctg gcgctcaatc tgccgggtgc cgcatacctc gtcgcgctca aggacatcgc
    13141 cgcggcgcac cacccgacca gcgtggatat cgctctcgtc gtcggcttca acctcatcat
    13201 gttcctgctg gcagaaatcc cgctgctcgg gttgatcttc gccccagagc agacggtggc
    13261 gcgagtgaag cgcgtcaatg cctggttttt ggcgaacagc cgcaccatcg cggtcgtcct
    13321 gagtgccgtg ctcggagcgt tcctcataac gcgcggcatc gtgaactcct gacaaggccg
    13381 attcttcgga gtttcatacg cgcctcacac gctccgctga aggtcggatg gttggattgg
    13441 gcgagtgatc ggcacggacg gaaaggagga tcggtgaacc gccttcgccg cgccgctgcc
    13501 atcacggcgg cggccgggct cctgacggcc ggcggcgctc tcggaagcgg cctgctctca
    13561 ggcgccgccg gggcgaacac cgacacgacc ccgacgacga ctgccccggc ggcgtcgaat
    13621 ggtcaatccc ggggcggctc gggatccaac tcctcgagac ccccgtgcca ccacaacgcc
    13681 gcatccccgc aacagggatc cggcagcacc tagacgcgag cggggcggga cgagctcatg
    13741 cgaactcgat taccgagcga atcccgtcct gcgcctccat cagctcgaag ccgcggttca
    13801 cgtcgtcgag ccggatgcgg tgcgacacga acggatcgac gtcgatgtcg ccggcaaggt
    13861 agctctccac gaactcgggc acctggtccc ggcccttcac gccgccgaag gaggacccgg
    13921 ccacgcggcg gccggtgatc agcaggcgcg ggacgatgtc gagcgtctca cccttgcctg
    13981 ccaccccggc gaccgtgcac agtccccaac ccatgcgcgc ggcctggacg gcctggcgca
    14041 tcacggcgac gttgcccgtg gcctcgaagg tgtagtcggc gccgaagccg cccgtcatct
    14101 cgaggatgcg ttccaccacg tccgggccgc cctgcatgac gtccgtggca ccctggccgc
    14161 gcgcgagctc gagccggtcg tcggagaggt ccacgcacac gatccggtcg gcgccctgga
    14221 gacggcagcc cgccacggcg ccgaggccga ccatgcccgc gccgaagacg atgcacgtcg
    14281 agccgggttc gacctcggcg gtgttaatcg cggcgccgag gccggtcgag aggccgcacg
    14341 cgaacaggca ggcgcggtca accggcgcat ccggattcac cctggcgagc gcgatctcgg
    14401 gcatgacggt gtactccgcg aacgtggaag tgcccatgaa gtgccggacc ggctcaccgt
    14461 tgcgcgagag gcgggcggtg ccgtcgggca ggaagccact gttctgctgc tcgcgaatcg
    14521 ccaggcacag attcgtcctg tcgctcaggc agtggacgca ctcccggcac tgcggcgaga
    14581 agagcgtgac cacgtggtcg cctggcgaga gcgaggtcac gccatcaccg atcttctcca
    14641 ccacacccgc gccctcgtgc ccgagcacgg tgggcgcata cccggaggga tccgcgccgg
    14701 aggccgtgta gagatcggtg tggcagaccc cgcacgcctc gaggcggacc agcacctcgc
    14761 ccgccttcgg ctctgccaga tccagctcct gaacctcgag cggggcaccg aactcctcga
    14821 gcacagccgc gcgaatcctc atctccaagc tcctcctctc gccgggcgtc ggtgagtatc
    14881 caacggcggg ctctgcgggc gcaacatcgc cctgcagccg gtgtccggtc tgccgatcac
    14941 cgaggcgatg acgcccgccg aagccaccca cgagtcagcc cccagcgagt tcgtgctcta
    15001 cgaggagtac ctcggcgtgt acctggcgat gcgcccgggc gacggcggct ggctcatcga
    15061 gacccgcaag acgccggatg gggaggcagt cgccagcttc aagcgcccgc ttgtgcacgt
    15121 gctggagtgg ctcacgagtg agaaggcggc gtgggcgctg tcctcgcacc gcgacaagct
    15181 cgaggccgtg cgcgcagccg cgaaggagct gctccgctcg ctgccacccg gctcgttcat
    15241 cggggccgag gaggtgggcg cgatcgagct cgccgccgac cgcatgcagg tgctgcggcg
    15301 cgatctcgac gaggtggtgg atcacgaagg gcgcgcgcgg cgacggcgcc gagtggagga
    15361 gatggtcgcg tctggagaga tcggggcgac accgagttcg aggacgaaga gcgcatcgcg
    15421 agcttcgagg gacggcgcaa cggccggccg gtgctctgcg atctcgagcc gtcggggtgg
    15481 cggctgcgcg gctccctcat ctcagtcacg ctcggcgaag cactcgagct gatcggctca
    15541 gtggagcgcc aggcggccat cgcccgccat catcgcgcgc tcgaactggc gcgccgcgcc
    15601 gcgctgaaga cgctggagga cgtgccgctc ggcgactggc ccgaggtact cgagaccgcg
    15661 gcggcgatgt acacgttcac cgggctggcg cgcgagatcg aggaagcggt cgaggtacgc
    15721 agcgccgccg acgttccgct ctactaggca ggctcagacg agccgcggca cggcctgtgc
    15781 cgtgagcacc gctcccgcac acacgatcac gagggccgac accgccggca gcgccttcac
    15841 gacgccgccg ccgaagaagc tcacgcgcgt cagcgcccgc cgcgcgtaga ccacggcgag
    15901 gcccagggcc gtgagcgtgc cggcgaggcc gaagctgaag gcgaggatca ggagcagccc
    15961 gagcgcgacc tcgtgctggg agattgcgcc gagcagcacc acgagcgcgg acgggcaggg
    16021 gatgaggccc gcggacgccc ccatcccgag cagccccttc cagctgatct catcgggcgg
    16081 gtggtggtga tcgtgatcgt ggtgatggtc gtgatcgtgg tgacgatgcg gatgcgcgcg
    16141 ggagcgtgcc cgcaaccgcg agcgcagcac cgcggctccg atgctcacga cgagcaggcc
    16201 ggaggcgagg ttcagccagg ggtagaggtc ctcaggcacg atgaactgcg acagcagcag
    16261 agtgactacg ccgagggcga acacgccgat cgtgtgggtg acagtgacgg tggcgccgag
    16321 cgccacggcg tgccgcggcc ggccgcgcgc gccgacgaga taggccgcga ccatcgcctt
    16381 gccgtggccc ggcgagagcg cgtgcaccgc gccccaggcg aacgcagcaa gcagcaggaa
    16441 caccagcaca ccgtgcccgg cgatggcatc gtcgagcacc ttcgtgaagc cgtcgccgga
    16501 ccggtcatgc gtcacctgcg cgctcgcgaa cgggctgggc ggtgcgctca ccgtgccgtg
    16561 ccccggcttc gcgctgaacc gagcgctgcg ctggtcgagc ggcgacgaca gcgtgtcctt
    16621 cgggtaggcg cgcagcccac cggtcgggtc ggctgccggc acgctcgaag tcacggccgt
    16681 accccacccc ggctggatca cgatcgcctt ccagcccacc ctgccctcga aggtgccgtc
    16741 gtggatgtgt accgagcgcg cgccggccac gcgggcggag aggggaatct ccacccgcgt
    16801 caacttcagc ccgccctgtc cgggtgggaa gctgatcgac gctccgggct gcagctcgag
    16861 cggcaggcgg cggccgtcca ccgtcagctc gacgccgcgc atcacctccg tgcgctttcg
    16921 cgcgagcacc tcggcgggtg cgagcccgcg ttcctggaac gtcgggatct ctgcctggtc
    16981 gaggatgtag cgaacctgca cctggtcggc agacaccctc acctggtcga ggtggttgat
    17041 gctgaagttg ccgagcgggt gcgcgtgcgc cgccgccggg gcgagcccga ggagcgcgag
    17101 agcgaccagg acgagcagga tgcgcctcac cggagagcct cctgcgcttg gcgtgcgtaa
    17161 agcgggttga accgcgggtt gagctcgagc gagcgggcga ggtaggcgcg agcgcagtca
    17221 agccggcccg ccgcccgcgc cgccatgccg gcgtggaaca ggaacatcgg atcgcgcgag
    17281 ccgagccgca gcgcgcgctg tgcccagcga agcccggcgc ccggtcgccc cgcgcgcgtg
    17341 tacgcccagc cgagtgcgtc ggcggagcgc acgctcgacg cctcactcca agcgcgccgc
    17401 gccagcatga cggcccgctt agggctgccg tggctcgcct cgaacagcgc gagatccacg
    17461 tcggtgttca cgccgttcgc ctgcagcagc cgctcctcag ctcgcacgag ggcgaggtcg
    17521 tggcgcgcct gcgcacgccg ccccgcggcc tgctccgtct cgccgaggcc caccacgtat
    17581 tcgggcagcg gcagccgcgc gaccgcggag ctgtatcggc gtatcgcggc accgaggtcg
    17641 ccgcgcgccg cctccacacg cgcgagcccg gcgctcgccg gcacgtagcc cgggaagcga
    17701 gcgagcgcgg tccggtatgc ctcgcgcgcg gcgccgaggc gcccggtgtc gaagagcagg
    17761 ttgccgagga gcgtctgcac atacgcgacg ttctcgg
//
LOCUS       DASVXS010000238         7924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_66134, whole genome shotgun sequence.
ACCESSION   DASVXS010000238 DASVXS010000000
VERSION     DASVXS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66134"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VF752_13520"
     CDS             <1..493
                     /locus_tag="VF752_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856610.1"
                     /translation="GAPITLGAVLRVKHGDKTFIAHPSRNYYPSQDLAQGGTFGRFFE
                     GEANSEIAMTWGLSKNFWAAVTPDISPLMKPIAVANKKFSKSSPDEQGLILAAITESY
                     ASKAPPATFRLIVSPMVSWIWIGGAIAILGALMAGWPSPEARRRRVTSLYAARLGRDL
                     SRA"
     gene            500..814
                     /locus_tag="VF752_13525"
     CDS             500..814
                     /locus_tag="VF752_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856611.1"
                     /translation="MDLVLVVIVLVVVVAFIVAGPLRRGAESDRREDERRAALEAAKE
                     AKYREIRDAELDFQMGKLSEADHRAVDRELRGQAIEILRQIDELEGAEGDERPPATSA
                     GR"
     gene            825..1055
                     /gene="secG"
                     /locus_tag="VF752_13530"
     CDS             825..1055
                     /gene="secG"
                     /locus_tag="VF752_13530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX8856612.1"
                     /translation="MVVGILSGLQVFVSIVLIMLVLMHSGKDAGLSGAFGVGTGGGPF
                     GGGSLVERNLDRWTIFFAIVFVVNVIVLLKVD"
     gene            complement(1052..2419)
                     /locus_tag="VF752_13535"
     CDS             complement(1052..2419)
                     /locus_tag="VF752_13535"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX8856613.1"
                     /translation="MSEPALDIAERALGAVREGDGAQATVVAERSLVLRFARSRPTQS
                     TAVDDVTVELSVLREGHLGSATTNRTDSESLEQCARSAEAAAEATARSRGAGDYPGFP
                     PPRKCRPHRGFDPDTAALDPAPGGHALRTVFEAANDHGVEAHGVWTDGDVTTAIASST
                     GTALNDRVTDAFMKVVCISPSGRSGFATASGVRLSAIDPAELAFKAAAKATVPGEPAR
                     LEPGEYPVVLEVPAAAELLTWLGHVAFNGLAYAEDRSPLNGSLGALVSAPSINLSDSP
                     RYRRTIPRAFDFEGVPKAPLPLIQDGVAHRVVHDTRSAALTGDGATSTGHALAPGGDD
                     WGPIPTNLVLIGGGAADEAELCLPIELGVYVTRVWYTNMLRPKETLLTGVTRDGTFLI
                     EDGRVTRPLEDMRFTDSVLRVFEGTQALTSRTRLWSEGEFYGRRFATGVVTPSVRSAS
                     MRFTA"
     gene            complement(2416..3855)
                     /locus_tag="VF752_13540"
     CDS             complement(2416..3855)
                     /locus_tag="VF752_13540"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8856614.1"
                     /translation="MKDVLQELMAAAAGIVDYADARYVSSSSERLGVRNGRVLELARS
                     DGEGFGVRVRMHGAWGFAAARGSDRAAAESALARAIAIAESQPSPGDAPALAPEEPAL
                     GSHSSPSDRDPFDVSFEDKLSVLTAAEEQLRDDPRITVSTAHYVAFQDRKAFASTEGA
                     SYDQTTTECGGGVVATAVDSGETQVRSYPASFRGDVAQRGFEHFTELDLVGHAPRVRD
                     EALALLSAPACPSQRTTLILDGEQLALQVHESVGHAVELDRVLGLEASYAGTSWVEVT
                     GLGSLRYGSELMNVTADATTPEGLGSFAWDDEGVAAHRVPIVRDGVLRGFLSSRETAG
                     EVGLERSGGAMRADGFARQPIVRMTNVHLEPGDAGSLEDLIASTDRGILMETNRSWSI
                     DQRRWQFQFGTEIAWEIVDGRRGRMLRNPSYAGVTPEFWGSLDAVCSAPEWRLWGVLN
                     CGKGEPGQSMHVSHGTAPARFRDVQVGIA"
     gene            3953..5029
                     /locus_tag="VF752_13545"
     CDS             3953..5029
                     /locus_tag="VF752_13545"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX8856615.1"
                     /translation="MRALERLYPRTTIARLAIIGALLAGIFFVRANVVVEPGPLFLVP
                     LVLGAQWFGRIGGVSVGLIAAFVYAGARAVEAPQEVVDLVVATFVRVLLYAGVGYLIG
                     RLYEQRAMLSEMVATQARELVELREVQAALAPPEAPPRPALDLASVYVPAEEGAAGDF
                     YLVAPGPGDTTVMVVGDVAGHGLSAAKRAAFVRTTLATSAPYTDDPCKLLSLANTALL
                     ERAEPTEELVTAACLVYSPAEHRVRWALAGHPPPMWLDCGSPLASLRPSFPLGVEPDL
                     VCEPGEMSVEPGRGFLMFTDGLSEARRAGGELFGTDRITTVVSGLSGQSPSEVVSRLR
                     NAAEEFSGGHLPDDLCVVAARAAA"
     gene            complement(5032..5502)
                     /locus_tag="VF752_13550"
     CDS             complement(5032..5502)
                     /locus_tag="VF752_13550"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856616.1"
                     /translation="MRPAKIAVTAVLALAALALPASATAKSHGTRAKLDRNRDSLPDK
                     WEKRYGLSLKVKQAKKDQDRDGLNNRQEYKAGTNPRRADTDGDGLKDGDELKEGLNPL
                     SDDTDDDGFSDGEDADPTTADDGSGDDDLSDEDVGDDDSGDDLSDDPADDPADE"
     gene            5687..7207
                     /locus_tag="VF752_13555"
     CDS             5687..7207
                     /locus_tag="VF752_13555"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8856617.1"
                     /translation="MNGNSRFERDWHGVPEELSGRALEAHLSRREMLLRSASTLGAAA
                     VAASLPASTILAETASATAKANRLPAPSELPIDHFVILMMENRSFDHYFGWFPGADGS
                     QLQTYEDPQGVSHSTFHAADLGRAQWQGCGHPDPGHGWDAGRAQLRGGFLADGSGNDE
                     FALSYYEEGDLGFIQPAAKAFTLYDRYFCSLLGPTWPNRYCKWSAQSGGKKDNSPPAD
                     TLGNQWETIFDRALKNNPANATGPGLITARYYNSDLPFSAVWGPRGVSWTRPISEYYA
                     DCAAGTLPNITIVDPPFRDGGGFDGMSADEHPLGDVRLGQAFMADVVHAFIDSPNFRR
                     GALFVIYDEWGGFFDHVRPPSVPDARQSSNLDENFGQMGFRIPAVCVSPYTLGGRVAH
                     AQLGHESILKLIEYRFGLGSLTTRDQLTTNIGETFNWRAKPNAEVPDLPRPQHIASQP
                     CSTGGGDVIDADSAHARDIAALADLAERYGFTIGDGAAHELFREPDSVKQALAAGG"
     gene            complement(7204..>7924)
                     /locus_tag="VF752_13560"
     CDS             complement(7204..>7924)
                     /locus_tag="VF752_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8856618.1"
                     /translation="FLDGARRTLAEADRAVDDAKRTGAGELGRLRLAFSWSMRFDTLP
                     AVGRVLRAKHPGIQLLVQEMWNAKMAPAFADGSIDLALSLCPELSPGLEVEPIRRERL
                     VALLPNSHRLSSQSALPLSALAGEEFVLFPREVGPRLHDAFMAVYRHAGFEPHIRPES
                     FHTGWDVGGLSEVPAVALAPESVVSGLPPTLTAVRVSEPDDTLETCIVWRAAARSPAI
                     DTCIAAAHEALAASPRGVGVG"
BASE COUNT         1122 a         2789 c         2814 g         1199 t
ORIGIN      
        1 gggagcgccg atcacgctcg gcgcggtgct gcgcgtgaag cacggagaca agacctttat
       61 cgcgcatccc tcgcgcaact actacccgtc gcaggacctc gcacagggcg gcaccttcgg
      121 gcgcttcttc gagggcgagg ccaactccga gatcgcgatg acgtggggcc tctcgaagaa
      181 cttctgggcg gccgtcacgc cggacatctc gcccctcatg aagccgatcg ccgtggccaa
      241 caagaagttc tcgaagtcga gccccgacga gcaggggctg atcctcgcgg cgatcaccga
      301 gagctacgcg agcaaggccc cgcccgccac gttccgcctg atcgtctcgc cgatggtgtc
      361 gtggatctgg atcgggggcg cgatcgcgat cctcggtgcg ctgatggccg gctggccttc
      421 gcctgaggcg cgccggcggc gggtcacgag cctgtacgcg gctcggctcg gccgcgacct
      481 gtcgcgcgcg tagggcgtca tggatctcgt gctcgtggtg atcgtgctcg tggtcgtggt
      541 cgccttcatc gtggccggac cgctgcggcg cggcgccgag tccgaccgcc gcgaggacga
      601 gcggcgcgcc gcgctcgagg ccgcgaagga ggccaagtac cgcgagatcc gcgacgccga
      661 gcttgacttc cagatgggca agctctcgga ggccgaccac cgggccgtgg accgcgagct
      721 gcgcggccag gcaatcgaga tcctgcgcca gatcgacgag ctcgagggcg ccgagggaga
      781 cgagcgcccg cccgccacgt cagccggccg ctaagctcac cgccatggtc gtaggcattc
      841 tcagcggtct tcaggtgttc gtctcgatcg tcctgatcat gctggtgctc atgcattccg
      901 ggaaggacgc cgggctctcc ggcgcgttcg gagtcggcac gggcggcgga cccttcggtg
      961 gcggctcgct ggtcgagcgc aacctcgacc gctggacgat cttcttcgcg atcgtgttcg
     1021 tagtgaacgt gatcgtgctg ctgaaggtcg actaggccgt gaagcgcatc gacgctgagc
     1081 ggaccgacgg cgtcaccacg ccggtcgcga aacggcgacc gtagaactcg ccctcgctcc
     1141 acagccgcgt ccgtgaggtg agggcctgcg tgccctcgaa cacgcgcagg acgctgtcgg
     1201 tgaagcgcat gtcctcgagc gggcgcgtca cgcggccgtc ctcgatcagg aaggtgccgt
     1261 cgcgcgtgac gccggtgagg agcgtctcct tcggccgcag catgttcgtg taccagacgc
     1321 gcgtcacata gacgccgagc tcgatcggca ggcacagctc ggcctcatcg gccgcgccgc
     1381 cgcctatcag cacgaggttg gtcgggatcg gcccccagtc gtcaccgcca ggcgcaagcg
     1441 cgtgcccggt cgaggtcgcc ccgtcgccgg tgagcgccgc gctacgcgtg tcgtgcacca
     1501 cccgatgcgc cacgccgtcc tggatcagcg gcagtggtgc cttcggcacg ccctcgaagt
     1561 cgaaagcccg cgggatggtg cggcgatagc gcggcgagtc ggagaggttg atcgaggggg
     1621 cgctgacaag cgcgccgagc gagccgttca gcgggctgcg gtcttcagcg taagcgaggc
     1681 cattgaacgc cacgtgcccg agccaggtga gcagctcagc cgcggccgga acctcgagca
     1741 ccaccgggta ctcgccgggc tcgagcctgg ccggctcacc gggcaccgtg gcctttgcgg
     1801 cggccttgaa tgcgagctcg gctgggtcga ttgcggacag gcgcacgccg ctcgccgttg
     1861 cgaagccgct gcggccgctc gggctgatgc agacgacctt catgaacgcg tcggtcacgc
     1921 ggtcgttcag ggcggtgccg gtggaggagg cgatcgcggt cgtcacgtcg ccgtcggtcc
     1981 acactccgtg ggcctcgacg ccgtgatcgt tggcggcctc aaagaccgtg cgcaaggcgt
     2041 ggccgcccgg cgccggatcg agggccgccg tgtcgggatc gaagccgcgg tgcgggcggc
     2101 acttgcgcgg tggcgggaaa ccgggatagt cgcccgcgcc gcggctgcgc gccgtcgcct
     2161 cggcggccgc ctcggccgag cgtgcgcact gctcgagcga ctcgctgtcg gtgcggttcg
     2221 tggtggcgct cccgaggtgg ccttcgcgca gcacgctcag ctcgacggtc acgtcgtcga
     2281 ccgccgtcga ctgcgtggga cgagagcggg cgaagcgcag cacgagcgag cgctcggcca
     2341 ccaccgtcgc ctgcgcaccg tcgccctcgc ggaccgcacc gagagcgcgc tccgcgatgt
     2401 cgagcgcggg ctcgctcacg cgatgcccac ctgcacgtcg cggaagcgcg ctggagcggt
     2461 tccgtgcgag acgtgcatcg actggcctgg ctcgcccttg ccgcagttga gcacgcccca
     2521 tagccgccac tccggcgcgg agcagacggc gtcgaggctg ccccagaact ccggggtcac
     2581 gcccgcgtag ctcgggttgc gcagcatgcg cccgcgccgg ccgtcgacga tctcccaggc
     2641 gatctcggtg ccgaactgga actgccagcg acgctgatcg atcgaccagg agcggttggt
     2701 ctccatcagg atgcctcggt cggtggaggc gatcaggtct tcgagcgagc cggcatcgcc
     2761 cggctcgagg tgcacgttgg tcatgcgcac gatcggctgg cgcgcgaagc cgtcggcgcg
     2821 catcgcgccc ccggagcgct cgaggccgac ctcgcccgcg gtctcgcgcg aggagaggaa
     2881 gccgcgcagc accccgtcgc gcacgatcgg cacgcggtgt gcggccacgc cctcgtcgtc
     2941 ccaagcgaag ctgccgaggc cttcgggcgt ggtggcgtcg gcggtcacgt tcatcagctc
     3001 ggagccgtag cgcagagagc cgagccctgt cacctcgacc cagctcgtgc ccgcgtacga
     3061 ggcctccagg ccgagcacgc ggtcgagctc caccgcgtgc ccgaccgact cgtgcacctg
     3121 gagggcgagc tgctcgccgt cgaggatcag agtcgtgcgc tgcgacgggc aggccggcgc
     3181 gctcagcagc gcgagtgcct cgtcgcgcac gcgcggtgcg tggccgacga ggtcgagctc
     3241 cgtgaagtgc tcgaagccgc gctgcgccac gtcgccgcga aacgaggcgg gatacgagcg
     3301 cacctgcgtc tcgcctgagt cgaccgcggt ggcgacgacg ccgccgccac actcggtggt
     3361 cgtctggtcg tacgacgcgc cctcggtcga cgcgaacgcc ttgcgatcct ggaaggccac
     3421 gtagtgggcg gtcgacacgg tgatgcgcgg gtcgtcccgg agctgctcct ccgccgcggt
     3481 cagaactgag agcttgtcct cgaacgacac gtcgaagggg tcgcgatcgg aaggtgacga
     3541 gtggctgccc agcgccggct cctcgggcgc gagcgccggc gcatcgccgg gcgagggctg
     3601 cgactcggcg atcgcgatcg cgcgcgcaag cgcgctctcg gcggcggcac ggtcgctgcc
     3661 gcgcgcggcc gcaaagcccc acgcgccgtg catgcggacg cgcacgccga agccctcccc
     3721 gtccgaacgc gcgagctcga gcacgcggcc gttgcgcacg ccgaggcgtt ccgaggacga
     3781 gctcacgtag cgcgcatcgg cgtagtccac gatgccggcc gctgccgcca tcagctcttg
     3841 aagaacgtcc ttcatacggc ggcggaggct atcgggcagc gttgctggcg cagacgccga
     3901 agccgggttt aggatcgaag tgacggctgt ttcattgagg gggactcgcg acgtgcgcgc
     3961 ccttgagcgc ctctatccac gcacgaccat tgctcgcctg gcgatcatcg gcgcgctgct
     4021 cgccggcatc ttcttcgtgc gcgcgaacgt cgtcgtcgag cccggtccgc tcttcctcgt
     4081 tccgctcgtg ctcggcgcgc agtggttcgg gcggatcggc ggggtctccg tcgggctgat
     4141 cgctgccttc gtgtacgcgg gcgcgcgcgc ggtcgaggca ccgcaggagg tcgtcgacct
     4201 cgtggtcgcg actttcgtgc gcgtgctgct ctacgcgggc gtgggctact tgatcgggcg
     4261 cctctacgag cagcgcgcga tgctctccga aatggtcgcg acccaggccc gcgagctcgt
     4321 cgagctgcgc gaggtgcagg cggcgctcgc gccgccggag gcgccgccgc gtccggcgct
     4381 cgacctcgcg agcgtgtacg tcccggcgga ggagggagca gccggtgact tctacctggt
     4441 ggctccgggc ccgggcgaca cgacggtaat ggttgtaggc gacgtggcag gccacggcct
     4501 ctcggcggcg aagcgcgccg cgttcgtgcg caccacgctc gccacgtcgg cgccctacac
     4561 cgacgatccc tgcaagctgc tgtcgctcgc gaacacggcc ctgctcgagc gtgcagagcc
     4621 gaccgaggag ctcgtcacgg ccgcctgcct cgtctactcg ccggccgagc acagggtgcg
     4681 ctgggcgctc gcggggcatc cgccgccgat gtggctcgac tgcggctcgc cgctcgccag
     4741 cctgcgcccg tccttcccgc tcggcgtcga gccggatctc gtgtgcgagc ctggagagat
     4801 gtcggtcgag ccgggccgcg gcttcctcat gttcaccgac ggcctcagcg aggcgcgccg
     4861 tgcgggcggc gagcttttcg gcaccgaccg catcaccacg gtcgtatccg ggctcagcgg
     4921 gcagtcaccg agcgaggtcg tgagccggct ccgcaacgcc gccgaggagt tctccggtgg
     4981 ccacctgccg gacgacctct gcgtggtcgc cgcccgcgcg gcggcctgac cctactcgtc
     5041 cgctggatcg tcggccgggt cgtcgctcag gtcgtcgcct gagtcgtcgt ccccgacgtc
     5101 ctcgtcgctg agatcgtcat cgcccgagcc gtcgtcggcg gtagtcgggt cggcgtcctc
     5161 gccatcgctg aaaccgtcgt cgtcggtgtc gtcgctcaga gggttcaggc cctccttgag
     5221 ctcatcgccg tccttcaatc cgtcgccgtc ggtgtccgcc ctccgcggat tcgttccggc
     5281 cttgtactcc tgacggttgt tgaggccgtc gcggtcctgg tccttcttgg cctgcttcac
     5341 cttcagcgag aggccgtagc gcttctccca cttgtcgggc agcgagtcac gattgcggtc
     5401 gagcttcgca cgggtgccgt gactcttcgc ggtggcgctc gcgggaaggg cgagcgccgc
     5461 aagcgcgagc acggcggtca cggcaatctt ggcgggcctc atgcgcggtt gatcggccgc
     5521 gcgtcgctcg aacgtgagcc aggcgtgccg tcgctggacg ctgaacgacc gcgataacaa
     5581 ccaagtgacg ccagcgccgc ggcggtctgg taataacgca ctgccaggca ggggaggcgg
     5641 gacgagccgc gagcggcaaa gcccgcccac gagacaggag gcagtcttga acgggaactc
     5701 tcgcttcgag cgggactggc acggcgtgcc ggaggagctg tcgggtcggg cgctcgaggc
     5761 tcacctgagc cgccgcgaaa tgctgctgcg cagcgcgtcc acgctcggag cggcggctgt
     5821 cgccgcgtcg ctgccggcca gcacgatcct ggcggagacg gcgagcgcca cggcgaaggc
     5881 gaaccggttg cccgcgccat ccgagctgcc catcgatcac ttcgtgatcc tgatgatgga
     5941 gaaccgctcg ttcgaccact acttcggctg gttcccgggc gccgacggca gccagctgca
     6001 gacctacgag gatcctcagg gcgtctcgca cagcaccttc cacgctgcgg atctcggtcg
     6061 cgcgcagtgg cagggctgcg gccaccctga tccgggccac ggctgggacg ccggccgcgc
     6121 ccagctacgc ggcggcttcc tcgcagacgg cagcggcaac gacgagttcg ccctctccta
     6181 ttacgaagag ggcgacctcg gcttcatcca gccggcggcg aaggcgttca cgctctacga
     6241 ccgctacttc tgctcgctgc tcggcccgac ttggccgaac cgctactgca agtggtcggc
     6301 ccagtccggc gggaagaagg acaactcgcc gccggccgac acgcttggaa accagtggga
     6361 gacgatcttc gaccgggcgc tcaagaacaa cccggccaac gcgacgggcc ccgggctgat
     6421 cacggctcgc tactacaact ccgatctccc gttctccgcc gtgtgggggc cgcgcggcgt
     6481 ttcctggacg cgcccgatca gcgagtacta cgccgactgc gcggccggca cgctgccgaa
     6541 catcacgatc gtcgatccgc ccttccgcga cggcggcggc ttcgacggga tgtcggccga
     6601 cgagcatccg ctcggcgacg tccggctcgg ccaggcgttc atggccgacg tcgtgcacgc
     6661 gttcatcgac tcgccgaact tcaggcgtgg ggcactcttc gtcatctacg acgagtgggg
     6721 tgggtttttc gaccacgtgc gaccgccgtc ggtgcccgac gcccggcaga gctccaacct
     6781 cgacgagaac ttcgggcaga tggggttccg catccccgcg gtgtgcgtct ctccgtacac
     6841 gctcggcgga cgggtcgcgc acgcgcagct gggccacgag tcgatcctca agctgatcga
     6901 gtaccggttc gggctcggca gcctcacgac gcgtgaccag ctgaccacca acatcggcga
     6961 gacgttcaac tggcgtgcga agccgaacgc cgaggtgccc gatctgccgc ggccgcagca
     7021 catcgcgtcg cagccgtgct cgaccggcgg cggtgacgtg atcgacgccg acagcgccca
     7081 cgctcgggac atcgcggcgc tcgccgacct tgccgagcgc tacgggttca cgatcggcga
     7141 tggcgcagcg catgagctct tccgcgagcc ggactcagtg aagcaggcgc tggcggcggg
     7201 cggctaaccg acgccgacgc cccgcggcga ggccgcgagc gcctcgtgcg ccgccgcgat
     7261 gcacgtgtcg atcgcgggcg agcgggcagc ggcccgccag acgatgcatg tctcgagcgt
     7321 gtcgtcgggc tcgctgactc tcacggcggt cagcgtggga ggcaggccgc tgaccaccga
     7381 ctcgggcgcg agcgcgaccg cgggcacctc ggagaggccg cccacgtccc agcccgtgtg
     7441 gaacgactcg ggccggatgt gtggctcgaa tccggcgtgt cggtagaccg ccatgaacgc
     7501 gtcgtgcagc cgcggcccga cctcgcgcgg gaagagcacg aactcctcgc cggcgagcgc
     7561 cgacagcggc agtgcggact gcgacgagag tcgatgactg ttcggtagca gcgcgacgag
     7621 tcgctctcgg cgaatcggct cgacctcgag cccaggcgag agttcggggc acagcgagag
     7681 cgcgaggtcg atgctgccgt cggcgaacgc gggcgccatc ttggcgttcc acatctcctg
     7741 cacgagcagc tggatgcccg gatgctttgc gcgcaacacc cggccgactg cgggcagcgt
     7801 gtcgaatcgc atgctccatg agaaggcgag ccgcagccgg ccgagctcgc ccgccccggt
     7861 tcgcttggca tcgtccaccg cgcgatcggc ctcagcgagc gtgcgccgcg caccgtcgag
     7921 gaac
//
LOCUS       DASVXS010000239         4818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_104314, whole genome shotgun sequence.
ACCESSION   DASVXS010000239 DASVXS010000000
VERSION     DASVXS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104314"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VF752_13565"
     CDS             <1..464
                     /locus_tag="VF752_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX8856619.1"
                     /translation="GMPLSGACGLWDAGLGAQPIDPRRVVLVGVRDLDEAERDLLEHS
                     DVIVVGASLETLVFTQNALDRSPVYVHLDLDVLDPETFPAGFPAPGGLAPEKLYDLLE
                     AVAGECEIVGLEVTGFEAPEDELERQAHADTAAQVLEPLLDAIELDRVAVD"
     gene            717..2294
                     /locus_tag="VF752_13570"
     CDS             717..2294
                     /locus_tag="VF752_13570"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8856620.1"
                     /translation="MAATEAPQQSILRPLVEDLHERRERARLGGGPEKIERQHSLDKL
                     TARERLDLLLDEGTFTELGIHAGPHFSQRSMDGVEAPADGVITGYGKVDGRLVAVCAY
                     DFTVMAGAMGMTGEIKVSRLRDLALQKRIPFIWLLDSAGARIQEAAGSLFAGSGHLFR
                     EEVVMSGVIPQIAALMGPCAAGTAYIPGLADFVPMVKGRGSMALAGPHLTKAVTGEDV
                     TQEELGGSRVHCRVSGVGDLEVKSDEECIQRIKEYLSFFPQNCEQTPPVTPSADPAER
                     MDDELLDVLPETARKPYDMYDVIRRIVDDGFYFDMKPQFAKTIITCFARMGGRPVGIV
                     ANQPKHLGGILENDSADKAARFINLCDAFGIPLLFLQDVPGFMVGTKVEQEGIIRHGA
                     KMLYAVSRATVPKVTVIVRKAYGAGYYVMCGKAYEPDLIVAWPTAEISVMGPEGAVNI
                     IFRKQIEQAEDPDAKRAELVQGIREIIDPYIAAKNGMIDDIIDPRETRPVVIKALEMA
                     ASKRVERPWKKHGVMPV"
     gene            2306..2779
                     /locus_tag="VF752_13575"
     CDS             2306..2779
                     /locus_tag="VF752_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856621.1"
                     /translation="MALAVAAALAVAGCGGGSSGPTQKDYEKQFNAAIDKAQKTAGST
                     QPKSSKLADQAKALEARQTYVKQLADSIEPIKAPKEVTKAHTELVSGLRAYASEEQPL
                     IDLAKKGEKKKAFKLAASGTIPSKATLAKLRDALSIYKNENYKLKGVTTGAGING"
     gene            2776..3660
                     /locus_tag="VF752_13580"
     CDS             2776..3660
                     /locus_tag="VF752_13580"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX8856622.1"
                     /translation="MSVEDPVIITCSISGAVANRDQCPAIPYTPEEYAAEARRIVDEG
                     GSMIHIHARTPDGTPSYEVEDFRAITEAILAEVDDVIINYSTGAIGVPVEKRIEYLRE
                     LRPDVAALNMGSMNYAKYSRRRKDFVFKAVFENSFDTIIALLEPMRELGIRPEHECFD
                     SGHIANLDPLLDMGLLAEPLQISCVMGVTGGIRPTARNLAHMSEQISGGADGPNNWGV
                     IGISRDQWRLCAAALALGGNIRVGLEDNFYLPDGEMARSNGDLIAKARQMTEDVGRRP
                     ATVAEARELLGTPKRAAA"
     gene            3663..>4818
                     /locus_tag="VF752_13585"
     CDS             3663..>4818
                     /locus_tag="VF752_13585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8856623.1"
                     /translation="MGALEGIGVLDLSRLLPGGFCSLLLADFGADVLKVEDTGMGDYV
                     RWAPPYYEGADDSAKSALYLALNRGKRSIRVNLKEERGREVLLRLVRDADVLLESFRP
                     GVLDRLGVGYERLRKENPGLVYCAISGYGQDGPYRDRSGHDMNYLGLVGLLGLTGERD
                     GPPVQAAGQIADIGGGALMAAFGILAALRERDRSGEGQFVDVSMADGALSWLALVAGR
                     YFAEGVAPRRGDLELAGSIVCYRPYACKDGWVTLGALEPKFWQAWCRGVGREDLIEKQ
                     FERPGSETHAEVERIFLERTRDEWRAFAGEHDCCLEPVLGLDEALESELVRAREMVVE
                     LDQPGVEGAVRQLGVPMKLRRTPGGPAGPGPALGEHTREVLAAAGYSDEEI"
BASE COUNT          788 a         1626 c         1719 g          685 t
ORIGIN      
        1 gcggcatgcc gctgtccggc gcatgcggcc tgtgggacgc gggcctcggc gcgcagccga
       61 tcgacccgcg ccgcgtggtg ctcgtcggcg tgcgcgatct cgacgaggcc gagcgcgacc
      121 tgctcgagca cagtgacgtg atagtggtgg gtgcgagcct cgagacgctc gtgttcacgc
      181 aaaacgcgct cgaccgctcg cccgtctacg tgcacctcga cctcgacgtg ctcgaccccg
      241 agacgttccc cgccggcttc ccggccccgg gcggacttgc gcccgagaag ctctacgacc
      301 tgctcgaggc ggtggcgggc gagtgcgaga tcgtcgggct cgaggtaacc ggcttcgagg
      361 cgcccgagga cgagctcgag cggcaggccc atgcggacac tgccgcccaa gtgctcgagc
      421 ctcttctcga cgcgatcgag ctagatcgcg tggccgtcga ttaggtgctt gaagaacacg
      481 gactgcacgc cgcccgtgtc tccggtgaac gccgcgtccg cgtaggtcgt cgctcggcac
      541 cggcacgtcc tcgagggcgg cggcacgtcg taacccgagt ggggcacccc cacacagccg
      601 ggtcgcccta aagggccggt tcgcgtttcc ctacggccgt ttcttgacgc tccgcggggc
      661 gatccgtcac agtggggagt agcacttccc actttcgacg gaggacgccc acccatatgg
      721 cagcgacgga agcgccgcag cagtcgatcc tgaggccgct cgtagaggac ctacacgagc
      781 gccgcgagcg cgcacggctg ggcggcgggc cggagaagat cgagcgccag cactcgctcg
      841 acaagctcac ggcccgcgag cggctggacc tgctgctcga cgagggcacg ttcacggagc
      901 tcggcatcca cgcgggtcct cacttctcgc agcgctcgat ggacggcgtc gaggcgccag
      961 cggacggcgt catcacgggc tacgggaagg tcgacggccg cctggtcgcc gtgtgcgcct
     1021 acgacttcac cgtgatggcc ggcgcgatgg gcatgacagg cgagatcaag gtctcgcgcc
     1081 tccgcgacct cgcgctccag aagcggatcc cgttcatctg gctgctcgac tcggccggcg
     1141 cccgcatcca ggaggcggcc ggctcgctct tcgccggctc cggccacctc ttccgggagg
     1201 aggtcgtgat gagcggcgtg atcccgcaga tcgccgcgct gatgggtccg tgcgcggcgg
     1261 gcaccgcgta catccccggg ctcgccgact tcgtgccgat ggtgaagggg cgcggctcga
     1321 tggcgctcgc cggcccgcac ctcacgaagg ccgtgaccgg cgaggacgtg acgcaggagg
     1381 agctcggcgg ctcgcgcgtg cactgccgcg tgtcgggggt cggcgacctc gaggtgaagt
     1441 ccgacgagga gtgcatccag cgcatcaagg agtacctgtc cttcttcccg cagaactgcg
     1501 agcagacgcc gcccgtcacg ccgagcgccg acccggccga gcgcatggac gacgagctgc
     1561 tcgacgtgct gccggagacg gcgcggaagc cctacgacat gtacgacgtg atccgccgga
     1621 tcgtggacga cggcttctac ttcgacatga agccacagtt cgcgaagacg atcatcacct
     1681 gcttcgcgcg catgggcggc cgcccggtcg gcatcgtcgc gaaccagccg aagcacctcg
     1741 gcggcatcct cgagaacgac tccgccgaca aggcggcgcg cttcatcaac ctctgcgacg
     1801 cgttcgggat cccgctgctg ttcctgcagg acgtgcccgg cttcatggtc ggcacgaagg
     1861 tggagcagga gggcatcatc cgccacggcg cgaagatgct ctacgcggtc tcgcgcgcga
     1921 cggtgccgaa ggtcaccgtg atcgtgcgca aggcctacgg cgccggctac tacgtcatgt
     1981 gcggcaaggc gtacgagccc gacctgatcg tcgcgtggcc gaccgcggag atctcggtga
     2041 tgggtcccga gggcgccgtc aacatcatct tccgcaagca gatcgagcag gccgaggatc
     2101 cggatgcgaa gcgggccgag ctcgtacagg gcatccgcga gatcatcgac ccctacatcg
     2161 cggcgaaaaa cgggatgatc gacgacatca tcgatccccg tgagacgcgg ccggtcgtga
     2221 tcaaggccct cgagatggcc gcctcgaagc gtgtcgagcg gccctggaag aagcatggag
     2281 tgatgcccgt atgagacgtt ccgcgttggc cctcgcggtg gccgccgcgc tcgccgtcgc
     2341 aggctgcggc ggcggaagca gcggtcccac gcaaaaggac tacgagaagc agttcaacgc
     2401 cgcgatcgac aaggcgcaga agaccgccgg ctcgacgcag ccgaagtcga gcaagctcgc
     2461 ggaccaggcg aaggcgctcg aggcgcgcca gacgtacgtg aagcagctcg ccgactcgat
     2521 cgagccgatc aaggcgccga aggaggtcac gaaggcacac accgagctcg tctcgggcct
     2581 gcgcgcctac gccagcgagg agcagccgct gatcgacctc gccaagaagg gcgagaagaa
     2641 gaaggcgttc aagctcgcgg cgtcaggcac gattccgagc aaggccacgc tcgcgaagct
     2701 gcgcgacgcg ctcagcatct acaagaacga gaactacaag ctgaagggcg tcacgacggg
     2761 ggcggggatc aacgggtgag cgtcgaggac cccgtcatca tcacctgctc gatctcgggc
     2821 gcggtcgcca accgcgacca gtgcccggcg atcccctaca cgccggagga gtacgcggcg
     2881 gaggcgcggc ggatcgtgga cgagggcggg tcgatgatcc atatccacgc ccgcacgcct
     2941 gacggcacgc cgagctacga ggtcgaggac ttccgtgcga tcaccgaggc gatcctcgcc
     3001 gaggtcgacg acgtgatcat caactactcc accggcgcga tcggcgtgcc ggtcgagaag
     3061 cgcatcgagt acctgcgcga gctgcgcccc gacgtcgccg cgctgaacat gggctcgatg
     3121 aactacgcga agtactcgag gcgccgcaaa gacttcgtgt tcaaggccgt gttcgagaac
     3181 agcttcgaca cgatcatcgc gctgctcgag ccgatgcgtg agctcggcat ccgccccgag
     3241 cacgagtgct tcgactcggg gcacatcgcc aacctcgacc cgctgctcga catggggctg
     3301 ctagcggagc cgctccagat ctcgtgtgtg atgggcgtga ccggcggcat ccgcccgacg
     3361 gcgcgcaacc tcgcgcacat gtccgagcag atttcgggcg gagccgacgg cccgaacaac
     3421 tggggcgtga tcggcatcag ccgcgaccag tggcggctgt gcgcggcagc gcttgcgctc
     3481 ggcggcaaca tccgcgtggg gctggaggac aatttctacc tcccggacgg tgagatggcg
     3541 cgctcgaacg gcgacctgat cgcgaaggcg cgccagatga ccgaggacgt gggccggcgg
     3601 ccggccaccg tggcggaggc gcgcgagctc ctcggcacgc ccaagcgcgc ggcggcctga
     3661 ccatgggagc gctcgagggc atcggcgtcc tcgacctgtc gcggctcctg ccgggcggct
     3721 tctgctcgct gctcctcgcg gacttcggcg ccgacgtgct caaggtcgag gacaccggca
     3781 tgggggacta cgtgcgctgg gcgccgccct actacgaggg cgccgacgat tccgcgaagt
     3841 ccgcgctcta cctcgcgctc aaccgcggca agcgctcaat ccgcgtgaac ctgaaggagg
     3901 agcgcgggcg agaggtgctg ctgcgcctcg tgcgcgacgc cgacgtgctg ctcgagtcgt
     3961 tccgcccggg cgtgctcgac cgcctgggcg tgggatacga gcggctgcgc aaggagaacc
     4021 cagggctcgt gtactgcgcg atctcgggct acggccagga cgggccctac cgcgaccgtt
     4081 cgggccacga catgaattac ctcggactgg tcggacttct gggactgacc ggcgagcgcg
     4141 acgggccgcc tgtgcaggcg gcgggtcaga tcgccgacat cggcggcggc gcgctcatgg
     4201 cggcgttcgg gatcctcgcc gcgctgcgcg agcgcgaccg ctccggcgag ggccagttcg
     4261 tggacgtgtc gatggcggac ggcgcgctct cgtggctcgc gttggtggcc ggccgctact
     4321 tcgctgaggg agttgcgcca cggcgcggcg acctcgagct cgcgggatcg atcgtctgct
     4381 accggcccta cgcgtgcaag gacggctggg tcacgcttgg cgcgctcgag ccgaagttct
     4441 ggcaggcgtg gtgccgcgga gtggggcgcg aagacctgat cgagaagcag ttcgagcgcc
     4501 ccggctccga gacgcacgcc gaggtggagc gcatcttcct ggagcgcacg cgcgacgagt
     4561 ggcgggcgtt cgccggcgag cacgactgct gcctcgagcc tgtgctcggc ctcgatgagg
     4621 cgctcgaatc ggagctcgtg cgggcgcgcg agatggtcgt ggagctcgac cagcccggtg
     4681 tcgaaggtgc ggtgcggcag ctcggcgtgc cgatgaagct gcgtcgcacg cccggcgggc
     4741 cggcaggccc cggccccgcg ctcggcgagc acacgcgcga ggtgcttgcc gcggcgggct
     4801 attccgacga ggagatcg
//
LOCUS       DASVXS010000240         8919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1341000, whole genome shotgun sequence.
ACCESSION   DASVXS010000240 DASVXS010000000
VERSION     DASVXS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341000"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..316)
                     /locus_tag="VF752_13590"
     CDS             complement(68..316)
                     /locus_tag="VF752_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856624.1"
                     /translation="MSAAVWIMMGVAVWHFAVFVPDRFYGGIVGALLAAVAGAMAGGY
                     LASGLEVPTDNPPGVRQALLATPGAVFGLGLSYATGSR"
     gene            complement(403..1062)
                     /locus_tag="VF752_13595"
     CDS             complement(403..1062)
                     /locus_tag="VF752_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8856625.1"
                     /translation="MTTPLKTKVLLADDHTVVRRGLRMVLDAEPDLEVVAEAGDGAEA
                     VELGVSEPVDLAVLDVSMPRMTGLQAAAELNRRRPGVRVLMLSMHDNEQYLFEALKAG
                     AAGYVLKSAADRDLVDACRATMRGEPFLYPAAIAVLIRDYLDRAAAGDDVPDDPLTPR
                     ELEIVKLIAEGHTSGEIADLLVISPKTVDRHRANVLEKLGMRDRVELTRYAIRRGLVE
                     P"
     gene            complement(1059..2057)
                     /locus_tag="VF752_13600"
     CDS             complement(1059..2057)
                     /locus_tag="VF752_13600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8856626.1"
                     /translation="MNGLRHSPSLFWTVFAVNAAALTVAALLLLVTPVTIDAPVSVTQ
                     ALILVTGLVLMLALNLVFLRRAFRPLNRLEASMRSVDPLRPGRRMAAGSPVAEVSSLI
                     HSFNDMAHRLETERRESARRALAAQEGERRRLGRELHDQIGQTLTAVMLQVEQAKRAL
                     AQRRGGPGYPVETQLSEAVEFVRSSLEDVRRIARELRPEALDDLGLASALTALCVRFS
                     QGGLRVSRSIEADLGQLDADVELVTYRVAQEALTNVARHAGTSEAELRLRRRDGALVL
                     LVRDLGRGLPPEALDSRAGIGGMRERALLVGAQLAIRSEPEEGTEVELTVPLEAKR"
     gene            complement(2090..2605)
                     /locus_tag="VF752_13605"
     CDS             complement(2090..2605)
                     /locus_tag="VF752_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX8856627.1"
                     /translation="MDTAAADRGNNANGKRFILSAEALEAITAELDELRSTGRSEIAQ
                     RMREARSFGDGVESGESFAVSADASLLEARIARLEELVANARVVDSGVRGRGTVGVGS
                     RVRVKDAATGRVADYRVVPFDGTALSNGTVSVSSPVGQALMGARAGDLVRVELPSGRH
                     NTLRVLAVSRR"
     gene            complement(2775..3764)
                     /locus_tag="VF752_13610"
     CDS             complement(2775..3764)
                     /locus_tag="VF752_13610"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8856628.1"
                     /translation="MGWGTGGRTRRARVLAALGALALAAALAGCGGNGGSEAGGRVVV
                     ALDFTPNAAHAPIYLARHEGFDERHGVRLSIRKPGSGPDSLKLLAAGRADIAVLDIND
                     LGLARERGEDLVGVAALVQRPLAAIIAQPSIRRPRDLEGKRVGVSGLPSDPAFLRAVL
                     SRDGGNLAKVRQVTIGFGAVANMVTRKIAAVPAFWNAEGVELRQRGINVREFRVDDYG
                     APRYPEVLLVTTRRTLERRGDAIKRALAAVAEGVNATVSNRPLATSVIAAAAGNADPR
                     LVRAQVDAIAPALLPPLRLRRRTLEEWAAFDARTGLLPRKLDVASAFDFTVAP"
     gene            complement(3808..4437)
                     /locus_tag="VF752_13615"
     CDS             complement(3808..4437)
                     /locus_tag="VF752_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8856629.1"
                     /translation="MRELIVNTFLTLDGVMQAPGGPEEDPSEGFEHGGWSFGYWDGQM
                     EKSMEETMSKPFDLVLGRTTYEIFAAYWPRAEGPAAEPLNKATKHVASTTLKELDWQN
                     SKLIEGDLPAGVRALKEEDGPELQVHGSANLIQTLLEHGLIDEFRLYIFPVVLGKGKR
                     LFDGGAVPAGLELTHSDVSSTGVIMAIYRTGADIKYGSFADEAASETGA"
     gene            4526..4756
                     /locus_tag="VF752_13620"
     CDS             4526..4756
                     /locus_tag="VF752_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856630.1"
                     /translation="MKVQATVLLTFQAGSLAGAGSILDDVLERARERDDVEVGQVSVA
                     TPPGAAPVTLPPLSAPAEYPPGVPHTATFDRS"
     gene            complement(4762..5121)
                     /locus_tag="VF752_13625"
     CDS             complement(4762..5121)
                     /locus_tag="VF752_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856631.1"
                     /translation="MRTNHVFAGIAVGDLTVARAWYERLFGRGPDLIPNDSEVAWQLN
                     DSGWVYVVEDAERSGTALLTLLVDDLETVHGTLSERGIEVGSIEKIPGAAVQRMVLVD
                     PDGNRITLGEPVAAPEA"
     gene            5141..5782
                     /locus_tag="VF752_13630"
     CDS             5141..5782
                     /locus_tag="VF752_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX8856632.1"
                     /translation="MTTPELLVLPQRDPAFHQAVRRVKLLSWLSLVWMGAEGTIAIIA
                     GILAGSIALIGFGIDSAIEGFASAIIVWRFTGARIHSHAAERRAQKLVAVQFFLLAPY
                     VATEAIHKLVTAEHPSTSWLGIALTASSVIGMPLLGAAKIRLAHQLESAATKGEGNQN
                     LLCAYLAGAVLVGLIGNALFGLWWLDPLAALFIAGVAVREGRETWRGEGCACC"
     gene            complement(5786..6106)
                     /locus_tag="VF752_13635"
     CDS             complement(5786..6106)
                     /locus_tag="VF752_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856633.1"
                     /translation="MAALPAPVDAAIRAANDHDTDAFLACFSPDGVVDDWGREFPGPD
                     RIREWSDSEFIGVDVTLSVTDVNQSGDETVVNAEVGGRGFNGPSHFSFRLDGDRVSRM
                     TIRA"
     gene            6183..7016
                     /gene="aac(3)"
                     /locus_tag="VF752_13640"
     CDS             6183..7016
                     /gene="aac(3)"
                     /locus_tag="VF752_13640"
                     /inference="COORDINATES: protein motif:HMM:NF033082.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /protein_id="HEX8856634.1"
                     /translation="MEGSRAQQPEAVARPRLVADLRRLGLQLGGIVMVHTRMSAIGWV
                     IGGSETVVRALLEALGPDGTLAGYASWQEHVYHTTDWPETHREAYLADPPVFDRATGE
                     ATRDNGRIPERIRTWPGAERSAHPEASVVAVGSRANWLTSDHPQDDAYGKGSPFAKLV
                     EAHGQVLMLGAPLETVTLLHHAEAIANAPGKKRVRYTIPVVEGGGVTVRTYNDIDTST
                     GAFPYGDLGLGDDEFAVIARAALDAGIGVRGLVGQAQSHLFPAAELTAFAVEWLEERF
                     G"
     gene            7071..7637
                     /locus_tag="VF752_13645"
     CDS             7071..7637
                     /locus_tag="VF752_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX8856635.1"
                     /translation="MARAPTERPEGLVYEPDVLSPEDEAGLLERFEDLRFDPIVIRGQ
                     AARRTARHYGLSYDYESRTPQPGEPVPDWLLPVRARAAELAAVEPDELVEILVQRYPP
                     GSTIGWHRDAPAFDKVVGISLGGSSRLRFQRGKRDARRVFEVILEPRSGYLLAGEARR
                     FWEHSIPPTKETRYSITFRTLRRREPRR"
     gene            complement(7641..8168)
                     /locus_tag="VF752_13650"
     CDS             complement(7641..8168)
                     /locus_tag="VF752_13650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8856636.1"
                     /translation="MSREEARIEETDAGKVPADDGWFILNLSEIAWDTVPGGGTWCTF
                     ESPNARSPVLGIGVHVLEPGEPNGMYHGESNQEGFLVLSGECLLIVEGQERRMRQWDY
                     FHCPPFTEHITIGAGDGPCAILMVGLRSPDEKINYPAEPAAQKYGAAVQKATHSPKEA
                     YAGRPPFSPARSPFP"
     gene            complement(8165..8815)
                     /locus_tag="VF752_13655"
     CDS             complement(8165..8815)
                     /locus_tag="VF752_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8856637.1"
                     /translation="MGRLTFEISMSVDGYVAGPNADLEDPLGQGGELLHEWIFGTQAW
                     RESHGEEGGVHDADSELVAEAIANRGATIMGRRMFSGGEGPWEQDPNASGWWGDDPPF
                     RHPVFILTHHEREPVEFPNGTSFNFVTGGIEAALEQARAAAGDKDVGIGGGADVAQQY
                     LRAGLIDEMTLHVVPRLMGAGVRLFDGHLSGGGPQLERTRVLDSPNVTHLRYRVVK"
BASE COUNT         1347 a         3164 c         3039 g         1369 t
ORIGIN      
        1 gatgcctcga ggagcccgca ggcactgtcg tagaggcggt tatcgggcgt cgtgatcggc
       61 atcgccccta cctcgacccc gtcgcgtagc tcagcccgag gccgaagaca gcgcccggcg
      121 tcgccaagag cgcctgacgg acgcccggcg gattatcggt cggcacctcg agaccgctcg
      181 cgaggtagcc cccggccatg gcgcccgcaa cggccgccaa cagcgctccg acgatccctc
      241 cgtagaagcg gtccggcacg aacaccgcga agtgccagac ggcgacgccc atcatgatcc
      301 aaaccgctgc gctcacgcca cgcatgctgg actgcgtcgt cctgctgagc catgggttga
      361 gccacccatt tcacggccga gcggatctgc agttgtcgga ctctagggct ccaccaggcc
      421 ccggcggatc gcgtaccggg tcagctcgac ccggtcgcgc atgccgagct tctcgagcac
      481 gttcgcgcga tggcggtcca cggtcttggg gctgatcacg agcaagtcgg cgatctcacc
      541 gctggtgtgc ccttcggcga tcagcttcac gatctcgagc tctcgcggag tgagcggatc
      601 gtcgggcacg tcgtccccgg cggcagcgcg gtcgaggtag tcacggatca ggacggcgat
      661 ggccgccggg tagaggaacg gctcgccgcg catcgtcgcg cgacacgcat cgaccaggtc
      721 gcggtccgcc gccgacttca gcacgtagcc cgcggcaccc gccttgagcg cctcgaagag
      781 gtactgctcg ttgtcgtgca tggagagcat cagcacgcgc actccaggcc ggcgccgatt
      841 cagctctgcg gcggcttgga gacctgtcat gcgcggcatc gacacgtcga gcaccgcgag
      901 gtcgacgggc tcggacacgc cgagctcgac cgcctccgcc ccatcccccg cctcagccac
      961 cacctcgagg tccggctcgg catcgagcac catgcgcaga ccgcgccgca ccaccgtgtg
     1021 atcgtcggcc aggaggactt tggtcttcag cggggtcgtc accgctttgc ctcgagcggg
     1081 actgtcagct ccacctccgt gccctcctcc ggctcggaac ggatcgcgag ctgcgcacct
     1141 acgagcaacg cgcgctcccg cattccaccg attcccgcgc gcgaatcgag cgcttcgggc
     1201 ggcagtcccc tgcccaggtc gcgcacgagc agcacgagtg ctccgtcacg ccgccggaga
     1261 cgcaactcgg cctcggaggt gcccgcatgt cgcgccacgt tggtcagtgc ctcctgcgcc
     1321 actcggtagg tcaccagctc cacgtcggcg tcgagttgcc ccaggtccgc ctcgatcgag
     1381 cgcgacacgc gcaggccgcc ctgcgagaaa cggacgcaga gtgccgtcaa ggcgctcgcc
     1441 aggccgaggt cgtcgagcgc ctcgggtcgt agctcgcggg ctatccggcg cacgtcttcg
     1501 aggctcgagc gcacgaactc caccgcttcg gacagctgcg tctcgaccgg gtagccggga
     1561 ccgccacgac gctgagcgag cgctcgcttg gcttgctcga cctgcagcat cacggcggtc
     1621 agcgtctggc cgatctggtc gtggagctcg cgcccgaggc gccggcgctc gccttcttgc
     1681 gcggcgagcg cgcgccgcgc gctctcgcgg cgctcggtct cgagccggtg cgccatgtcg
     1741 ttgaacgagt ggatgagcga cgagacctcg gcgacgggcg atcccgccgc catgcggcgc
     1801 ccggggcgca gcgggtccac gctacgcatg ctcgcctcga gccggttgag cggccggaag
     1861 gcccggcgga ggaacacgag gttcagcgcg agcatcagca cgagaccggt cacgagaatg
     1921 agcgcctggg tgaccgacac cggcgcatcg atcgtgaccg gggtcaccag cagcagcagc
     1981 gcggcgactg tgagcgcggc ggcgttgacc gcgaacacgg tccagaagag cgagggcgag
     2041 tgacgcaacc cgttcatatc gacgagctaa gcagatcgag aaacacgctc tagcggcgcg
     2101 agacggcgag cacccggagc gtgttgtggc gcccggacgg cagctcgacg cgtacgaggt
     2161 cgccggctct cgcgcccatc agcgcctggc ccaccggcga gctcaccgag accgttccgt
     2221 tcgagagcgc cgtgccgtcg aatggcacca cgcgatagtc cgcaacccgg cccgtggccg
     2281 cgtccttcac gcgcacgcgc gagcccacgc cgacggtgcc ccgtccgcgg acgccggagt
     2341 caaccacgcg ggcgttcgcg accagctctt cgagccgggc gattcgcgcc tcgagcagcg
     2401 atgcgtccgc actcacggcg aacgactcgc cactctccac gccgtcgccg aaggacctgg
     2461 cctcccgcat tcgctgcgcg atctcactac ggcccgtgga gcgcagctcg tcgagctctg
     2521 cggtgatcgc ctcgagcgcc tccgcactca ggatgaagcg cttgccgttg gcgttgttgc
     2581 ctcggtcggc ggctgccgtg tccatgcccg gactatgagc agccggcgac acgcggcgta
     2641 tgggcgccag acaccatctt cgggcgcagc gcgggtgtat gacccgtcga gtcgaggtcg
     2701 cgctcaggtt cggatgagcg cgcccgaccg gcgcagcgca acgtgcgagc ggcaggtacg
     2761 gcccggcgcg tgtgttacgg ggcgacggtg aagtcgaacg ccgaagctac gtcgagcttg
     2821 cgcggcagga ggcccgtgcg agcatcgaac gccgcccatt cctcgagcgt ccgccgccgc
     2881 aggcgcagcg gaggcagcag agcgggcgcg atggcgtcga cctgagcgcg gacgagccgc
     2941 ggatccgcgt ttccggccgc agcggcgatc acggaggtcg caagcgggcg gttcgacacg
     3001 gttgcgttca ctccctcagc gactgctgcg agcgcacgct tgatggcgtc accgcgccgc
     3061 tcgagcgtgc gccgggtggt gaccagcaac acctcggggt agcgcggggc gccgtagtcg
     3121 tcgacgcgga actcgcgcac gttgatcccc cgctgccgca gctcgacgcc ctctgcgttc
     3181 cagaacgccg gaactgcggc gatcttccgg gtgaccatgt tggcgactgc gccgaagcca
     3241 atcgtgacct ggcgcacctt cgcgaggttc ccgccgtctc gcgagagcac cgcgcggagg
     3301 aacgccggat cggaagggag ccccgacacg cctacgcgct tgccctcgag gtcgcgcggg
     3361 cggcggatcg acggttgggc gatgatcgct gcgagcggcc gctggacgag cgcagcgacg
     3421 ccgacgaggt cctcaccgcg ttcgcgtgcc aagccgaggt cgttgatgtc gagaacggcg
     3481 atgtccgcgc gcccggccgc gagcagcttc agcgagtccg gtcccgagcc cggcttgcgg
     3541 atcgacagtc gaacgccatg gcgctcgtcg aatccctcgt gtcgagccag gtagatcggc
     3601 gcgtgtgcgg cgttgggcgt gaagtcgagc gcgacgacca cgcgcccgcc ggcttcggag
     3661 ccgccattgc cgccgcagcc ggcgagcgcg gcggcaagcg ccagtgcccc gagcgcggcg
     3721 agcaccctcg cgcgacgtgt tcggccaccg gtgccccagc ccatggcggg agtatcgacg
     3781 agagccgcct tatgcggctc tccggcgtca agcgccggtc tcgctcgctg cctcgtcggc
     3841 gaaggagccg tacttgatgt cggcgccggt tcggtagatg gccatgatca cgccggtgga
     3901 cgaaacgtcc gagtgcgtga gctccaggcc cgcgggcacc gcgccgccgt caaacaggcg
     3961 ctttcccttg ccgagcacga cggggaagat gtagaggcgg aactcgtcga tgagcccgtg
     4021 ttcgagcagc gtctgaatca ggttcgcgct gccgtgcacc tgaagctcgg gcccgtcctc
     4081 ttccttgagc gcgcgaacgc ccgcgggcag gtcgccctcg atcagcttcg agttctgcca
     4141 gtcgagctcc ttcagcgtgg ttgaggcgac gtgcttggtc gccttgttga gcggctcggc
     4201 ggcgggcccc tccgcgcgtg gccagtacgc cgcgaagatc tcgtacgtcg tgcggccgag
     4261 aacgaggtcg aacggcttcg acatcgtctc ctccatcgac ttctccatct ggccgtccca
     4321 gtagccgaac gaccagcccc catgctcgaa gccctcgctc ggatcctcct cggggccgcc
     4381 gggggcctgc atgacgccgt cgagggtcag gaaggtgttc acgatgagct ctcgcatgtc
     4441 cggctccttt cgcggggttc gatctggaga ccgccccagg atgggaaact catcggtgac
     4501 ggtcaatcgt ttgaaggtgg cgaagatgaa ggttcaagcc acggtgctcc tcacgtttca
     4561 agcaggctcg ctcgcggggg cgggttcgat cctcgacgac gtactcgagc gagcgcgcga
     4621 gcgcgacgac gtcgaggtcg ggcaggtctc ggtggcgacg ccgccgggcg ctgcgccggt
     4681 cacgcttccg cccctgtccg cgccggccga gtacccgccg ggcgtacccc acaccgcgac
     4741 gttcgatagg tcctagcggc ctcaggcctc cggcgcagcc acgggctcgc cgagcgtgat
     4801 ccggttgccg tccggatcca cgaggaccat ccgctgcacg gcggcgcccg ggatcttctc
     4861 gatcgagccg acctcgatcc cgcgctcgga gagcgtcccg tgaaccgtct cgaggtcgtc
     4921 caccagcagc gtgagcagcg cggtgcccga ccgctccgcg tcctcgacga cgtacaccca
     4981 gccggagtcg ttcagctgcc aggcgacctc tgagtcgttc ggaatcaggt ccggcccgcg
     5041 accgaacagc cgctcgtacc aggcgcgcgc gaccgtcaga tcgccgacgg cgatccccgc
     5101 aaagacatgg ttcgtccgca tcgtgcatcc tggatcttgc atgaccacgc ccgaactcct
     5161 agtcctgccg cagcgagacc ccgcgttcca ccaggcggtg cgccgcgtga agctcctgtc
     5221 gtggctgagc ctcgtgtgga tgggcgccga gggcacgatc gcgatcatcg ccggcatcct
     5281 ggccgggtcg atcgcattga tcggcttcgg catcgattcg gcgatcgagg gcttcgccag
     5341 cgcgatcatc gtgtggcgat tcaccggcgc gcgcattcac tcgcatgcgg ccgagcggcg
     5401 cgcgcagaag ctcgtggccg tgcagttctt cctgctcgcg ccatacgtcg cgacggaggc
     5461 gatccacaag ctcgtcacgg ccgagcatcc gagcacgtcg tggctcggca tcgccctcac
     5521 cgcgtcgagc gtcataggga tgccgctgct cggggcggcg aagatccggc tcgcccacca
     5581 gctcgaatcg gccgcgacca agggtgaggg aaaccagaac ctcctctgcg cgtacctcgc
     5641 cggcgctgtg ctcgtcggcc tgatcggcaa tgcgctcttc gggctttggt ggctcgatcc
     5701 cctcgccgcg ctcttcatcg caggtgtcgc cgtgcgcgag ggtcgcgaga cctggcgtgg
     5761 cgaaggctgc gcctgctgct gagagtcagg cccggatcgt catccgcgac acgcggtcgc
     5821 cgtccagccg gaacgagaag tggctcggcc cgttgaatcc tcgcccgccg acctcggcgt
     5881 tgaccacggt ctcgtcccca ctctggttga catccgttac ggagagcgtc acgtccaccc
     5941 cgatgaactc ggagtcgctc cactcgcgga tccggtcggg ccctggaaac tcgcgccccc
     6001 aatcgtcgac cacaccgtcc ggcgagaagc atgcgaggaa cgcgtcggtg tcgtggtcgt
     6061 tcgcggcgcg gattgccgcg tcgaccgggg ctggcagcgc cgccatcggg tccaaggttc
     6121 gcgcaacccg tcaaggcgat caagcgtggc gatatgcgaa ctgatgaagt gccgcggcag
     6181 cgatggaagg cagccgtgca caacagcccg aggccgtcgc tcgcccgcgc ctcgtagccg
     6241 atctgaggcg cctgggtctg cagttgggcg gcatcgtgat ggtgcacacc cgcatgagcg
     6301 cgatcggctg ggtaatcggt ggcagcgaga cggtcgtgcg cgcgctgctc gaggcgctcg
     6361 ggcccgacgg gacgctggcc ggctacgcct cttggcagga gcacgtgtac cacacgaccg
     6421 attggcccga gactcaccgc gaggcgtacc tcgccgatcc gccggtgttc gatcgcgcga
     6481 ccggcgaggc gacgcgtgac aacggccgca tcccggagcg gatccggacc tggcccggcg
     6541 ctgagcgcag cgcgcatccc gaggccagcg tcgtggcggt cggtagccgc gcgaactggc
     6601 tgacatccga tcatccgcag gacgacgcgt acgggaaagg cagcccgttc gcgaagctcg
     6661 ttgaggcgca cggacaggtt ctgatgctgg gcgcgccgct cgagacggtc acgctgctgc
     6721 atcacgcgga ggcgatcgcc aacgctcccg gcaagaagcg cgtcaggtac acgatcccgg
     6781 tcgtggaggg cggcggggtg acggtgcgca cctacaacga catcgacacc tcgactggcg
     6841 cgttcccgta cggcgatctc gggctcggag acgacgagtt cgccgtgatc gcgcgagccg
     6901 cgctcgacgc gggcatcggc gtgcgaggcc tcgtggggca ggcacagagc catctcttcc
     6961 ccgccgccga gctcacggcg ttcgccgtgg agtggctcga agagcgcttc ggctagctgc
     7021 gccggcgagc gcgttaccgg cccgatccga gcgggcatgt gtgaggcgga atggcgcgag
     7081 cgcccactga gcgacccgaa gggctcgtct acgagcccga cgtcctctcg cccgaggacg
     7141 aggccgggct gctcgagcgc ttcgaggatc tgcgcttcga cccgatcgtg atccgcggcc
     7201 aggcggcgcg ccgcacggcg cgccactacg gcctgagcta cgactacgag tcgaggacgc
     7261 cgcagcccgg cgagccggtt ccggactggc tgctgccggt gcgagcgcgg gcggcggaac
     7321 tcgcggcagt cgagcccgac gagctcgtcg agatcctcgt tcagcgctac ccgcccggtt
     7381 cgacaatcgg ctggcatcgt gacgcccctg ccttcgacaa agtcgttggc atctcgctcg
     7441 gcggctcgag ccggctgcgc ttccagcgcg gcaagcgcga cgcgcggcgc gtcttcgagg
     7501 tgatactcga gccgcgctcg ggctacctgc tcgcgggcga ggcgcgcagg ttctgggagc
     7561 actcgatccc gccgacgaag gagacgcgct actcgatcac cttcagaaca ctgcgcaggc
     7621 gcgagcctcg tcgctgagcg ctacggaaac ggcgagcgcg cgggcgaaaa cggaggccgc
     7681 cccgcgtagg cctccttcgg cgagtgggtc gccttctgga ctgcggcgcc gtacttctgg
     7741 gcagccggct cggccgggta gttgatcttc tcgtcgggcg agcgcagacc caccatcagg
     7801 atcgcgcagg gcccgtcgcc cgcgccgatc gtgatgtgct cggtgaacgg cgggcagtgg
     7861 aagtagtccc actgacgcat ccgccgttcc tggccctcca cgatcagcag gcactcgccc
     7921 gagagcacga ggaagccctc ctggttgctc tcaccgtggt acatcccgtt cggctcgccg
     7981 ggctcgagca cgtgcacgcc gataccgagc accggcgacc gcgcgttggg cgactcgaag
     8041 gtgcaccagg tgccgccgcc gggcacggtg tcccaggcga tctccgagag gttgaggatg
     8101 aaccacccgt cgtccgcggg gaccttcccc gcgtcggtct cctcgattcg cgcttcctcc
     8161 cgactcactt cacgacacgg tatctgaggt gcgttacgtt cggagagtcg agtacgcgcg
     8221 tgcgctcgag ctgcggaccg ccgccgctca agtgcccgtc gaagaggcgc acgccggcgc
     8281 ccatcagtcg tggcaccacg tggagggtca tctcgtcgat gagtcccgct cggaggtact
     8341 gctgcgccac gtccgctccg ccgcctatgc cgacatcctt gtcgccggct gccgcacggg
     8401 cctgctcgag cgcggcctcg atcccaccgg tcacgaagtt gaagctcgtg ccgttcggaa
     8461 actccaccgg ctcgcgctcg tggtgcgtga gtatgaatac ggggtggcgg aagggcgggt
     8521 catcgcccca ccagccgctc gcgttggggt cctgctccca gggcccttct cccccgctga
     8581 acatccgcct gcccatgatc gtcgcgcctc tgttcgcgat cgcctccgcg acgagctcgg
     8641 agtcggcatc gtgcacgccg ccttcctcgc cgtggctctc gcgccacgcc tgggtcccga
     8701 agatccactc gtgcaggagc tcgccgccct gccccagcgg atcctcgaga tcggcgttcg
     8761 ggccggccac gtagccgtcc acagacatcg agatctcaaa ggtcagtcgt cccatcgtga
     8821 ctccttccta taccgcctcg gagcactccg gctcggcgcg catgagctgg gcctccgccg
     8881 gcgaacggac cggcttcagg actgtcacgg cgatggcta
//
LOCUS       DASVXS010000241        11579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_66836, whole genome shotgun sequence.
ACCESSION   DASVXS010000241 DASVXS010000000
VERSION     DASVXS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66836"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF752_13660"
     CDS             <1..346
                     /locus_tag="VF752_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX8856638.1"
                     /translation="TALHDYAERVKSGAAPGNHAVVVGLLSASLSVACLDAVVGELYA
                     FSSSWVGAAMRLGLVDHRGAQALLHQARPVIATTALEVNDRDASRISSCTPFLDVMAM
                     RHEQAELRLFAS"
     gene            364..1080
                     /gene="ureG"
                     /locus_tag="VF752_13665"
     CDS             364..1080
                     /gene="ureG"
                     /locus_tag="VF752_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX8856639.1"
                     /translation="MTEPRHTHSHVHDNAHAPPLGNVRAPGVARIGIGGPVGSGKTAL
                     IEALVPRLLVAGHRPLVVTNDIFTREDAEHVRATLADVLDPQRVVGVETGSCPHTAVR
                     DDPSMNLDAIEELTDRFPDADVVLVESGGDNLTLTFSSALADYFIFVIDVAEGDKIPR
                     KRGPGVTSSDLLVINKIDLAPYVRASLDVMERDARAVRGGRPFVLTNCFTGDGLAQVI
                     HEISPVMNLYRPASSIRATP"
     gene            1077..2027
                     /locus_tag="VF752_13670"
     CDS             1077..2027
                     /locus_tag="VF752_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX8856640.1"
                     /translation="MTEPIALREDPEWYTPSTLPEEVMAFDDSLNGALDVGAAGKVGL
                     LDLAVSRHGGVTRVRRHYQRAPLHLYRPIYLDAGRADMAFLFVQQFGDGLVQGDRYRI
                     DVDCGPGSATHITTQAATNVFRARQNFATQLVNLRVAGGAVLEYLPDPVVPFRGSRLF
                     QRTCVTVDPEATLILGETLLPGRVAHDEAHVYDLYWSETEVRRPDGSLLFTDVLRLHP
                     SAGESPTSIGLLGAHDVIAALYLVSRRTDPADMVALLRIALAACRDVLAGVSELPNRS
                     GAAVKLLGRSSKAVHSALRTAWSAGRLELLGTPAPNLRKG"
     gene            complement(2031..3296)
                     /locus_tag="VF752_13675"
     CDS             complement(2031..3296)
                     /locus_tag="VF752_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8856641.1"
                     /translation="MCCTQGRGDRGIPPVAPAAGTISFGGLAIVAAAALAAPLVLGLF
                     PRIRLPAIVLEIVLGIVIGPQALGWVSIDTPIQVLSLLGLAFLLLLAGLEVEYERFRG
                     RPLRLTALGYAISFGLALLIGLGLHASGLVKSPLLIGIALSATSLGIVTPVLKDAGQV
                     GTPFGQLVVAGSSIAEIAPIVLLSLFFSGEASSFSAKLLLLGLFGLFVVAVGIAVFGA
                     EHSMRISATLLRLQDTTAEIRIRGSFLLLVVFAVLAERLGLEAILGAFLAGATLKLVD
                     RDQMLTHPQFRQKLEAVGYGVFVPAFFVATGVRFELNALFASATNLARVPIFLAALLV
                     ARGLPALIYKSLATRRQTLAAALLQATSLSSLVVAGQIGVQLDLIRPAAFAALVAAGL
                     LSVLLSPLTALTLLRGAGQKPEDLLEPAR"
     gene            complement(3251..3676)
                     /locus_tag="VF752_13680"
     CDS             complement(3251..3676)
                     /locus_tag="VF752_13680"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX8856642.1"
                     /translation="MTQASLYERLGGAFAIAAVIEHFSDAVVRNRIAGQESENPELRE
                     WSTDDPGRLPGLKFMRTLWVCDIAGGPQQYAATKPGHDRLALEEAHRRLKISPAVFDE
                     VAAELARSLDFFKVPEPEKGEVLAAFAAHKGEVTEGYPR"
     gene            complement(3696..3845)
                     /locus_tag="VF752_13685"
     CDS             complement(3696..3845)
                     /locus_tag="VF752_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856643.1"
                     /translation="MHAVEGLVALHAATVAVVGEIPLERLRHAVPPFPTRSELWLSLL
                     EQAGV"
     gene            complement(4082..4474)
                     /locus_tag="VF752_13690"
     CDS             complement(4082..4474)
                     /locus_tag="VF752_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8856644.1"
                     /translation="MLGEMTLRCVIVDDSPAVLRAASDLLEREGVAVVGVARTVQEAV
                     SLVEERKPDVVLVDIDLGRESGFDVARRLVQSLDSAESRTILISTHDEADFADLIAVS
                     PAIGFLPKSDLSATAIRRLLAHPRDEGR"
     gene            complement(4537..5232)
                     /locus_tag="VF752_13695"
     CDS             complement(4537..5232)
                     /locus_tag="VF752_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8856645.1"
                     /translation="MYRIGTTRSIHDRPANGSPSPPSAGALGKPPAGPDAASRQWVEQ
                     LRVGHPRHHQAVGSLHDLLRRVAISELSRRRPQLQSVTGPEFDDLAQQAADDALVKVL
                     DRIDDFRGLSRFTTWAYKFVIFEVSATVARHAWRRQPPGVEELAWDQLPDPLAPRPED
                     RMEQRAQLDALSQALAKLTDRQREVFVAIALNEVPIDIVALKLGTNRGAVYKNLFDAR
                     RNLRARMAAAGHP"
     gene            complement(5307..9230)
                     /locus_tag="VF752_13700"
     CDS             complement(5307..9230)
                     /locus_tag="VF752_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8856646.1"
                     /translation="MALVLGLTVAGFIVARVLAERDAKHGSERRIEIAAAQIRSRVEV
                     ATTLTDSLSRFMSDEGARGVTNTEFTRNASRWLSPADLPAAAWAQEVGAADRSAYERR
                     IGRRIVAPDERRKSVPTSSSYLPATLISGFPPMNSPGIDLRRELGIPAALDSAIQPGG
                     VGATPIAARRDGTRGLFLVAPAPNLIDGVLRPGAVVVFLSEATLRAAARNPAGLRFVP
                     AGRSSGNRAGGDTVREGFTVAGQQFAVLMPKESVSGPGAVLPWILLAGGLVVAAFAAA
                     LAVNTARRAKAQQDFDRIFNLSPDLVAVANFDGYLTRVNPAAEQVLGYTDEELLARPY
                     LDFVHPDDREKTAAEAADVGQGKTTLSFENRFFRKDGSLRVLEWTAAPVVEDGVMYGV
                     ARDVTDRRRAETESQRLADEQSALRRVAELVARHAAPERVFAAVTEQLSHLLGIDLIG
                     AVRFEPDGSATVLAERGRTDDRTAPGTKVPLSGGGVLEAMFRTGRPVRIEDYAKVPGP
                     FSAIVREEGVRCAVGGPIVVDGRLWGALVAGSQTAEALPPGSEDRVAQFAELVSTAIS
                     NIESRLQVERLAAEQSALRRVATLVAEGATPNRVFDAVRDEVEQMFGIPNTILMRFDP
                     GDIATLLATPGKYLGPVGRQWALEGDDSAVARVYRSGQAARADYTAGAQGPLAEAARL
                     GGTLFPVAVPVVVDGAPWGAMSVGSRGPKPPPPDLESRLAKFTELLGTAIANAESRAD
                     LAASEARARELASEQAALRRVAELVAAQPPPEQVFALVTEELSGLLGADLMVVTARFE
                     ADGTATTLAGHGMPKYMAPTPGTNVPLPEGSVVGKVLRTGRPARVDDYSRVPGPLGDS
                     VREQGVRAAVAGPIVVDGRLWGAMAVGSERSLPPGTEHRVAQFAELVSTAISNIESRA
                     KVERLAAEQSALRRVATLVASEHSPDDLFATLVEEVRVLLGVDASEILRYEADATVTV
                     VAAWSDAAISLPVGERLPLEGENLASEVKSTGVPGRKEDYSDAPGAIAALSREIGIRS
                     AVATPIVVEGATWGMIAVASRRPDPLPADTEARLAEFSRVAGMAVANAKSRSDLAQSR
                     ARIVRAGDEARRRFERDLHDGAQQRLVSLGLELRAAETTVPPELHDLRRVLSHLGTGL
                     ADVLDGLRELSRGLHPAVLSEGGLRSALRSLARRSAVPVDLRLDLDAQRFEEPVEVAA
                     YYVVSETLANTAKHAHASRAELRVRQRNGWLELIVSDDGRGGADASDGSGLTGLVDRV
                     EAIGGTIRIDSSPDAGTAVHVRLPTSARHRAALQDGGRFPGSRKG"
     gene            complement(9335..10327)
                     /locus_tag="VF752_13705"
     CDS             complement(9335..10327)
                     /locus_tag="VF752_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856647.1"
                     /translation="MNGNALEWPLTRRQVLAAAFAAGASVVVGCGSADKDAAGWTFVD
                     DRKQTVRLSKRPTRIVAYTTAAAALHQWGVTPVGVFGDNPEEDPVLAGFPWAESEIIG
                     SVYGEIDAAKLALLKAELIVSRWYPPPADSPLFGFKDLAQQKRIGSRVPIVGFSGHVI
                     ATKQIDRFGDLVRELGSDTMGGANGRARAAFLRAAASLSQIARRKSNLRIIAISGDQS
                     TMYVAKVVDSGELAFYRSRGVPLISAETSEAYWDRFSWDHAAKYPADGILYDARSLFL
                     PLRQAKQIPAFAALPAVRANQLGRWLVDTPPSYQAYTAAMDDLAAKIAGWHRIT"
     gene            complement(10419..11081)
                     /locus_tag="VF752_13710"
     CDS             complement(10419..11081)
                     /locus_tag="VF752_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856648.1"
                     /translation="MSSTEPVPTGPGAGRSVDVLDSVQEFGVFASTTEALTVTILTEL
                     VRRTSLHPEVPSSVPVIFDGVYAAERDHWQFIRKLYRPTTTRFWIPDAFFGGAGDALD
                     LTAVGQGVAAGEHLFVNTYLVGVTTCAAAGRPELARYSAELAAVESEHRVLGQTLAGA
                     SPPNDLGFAVFEFDRISQIKTALMSAGFGLGEQGKSAGRFYDLPQPPTAPRVPISSNE
                     PQ"
     gene            complement(11292..>11579)
                     /locus_tag="VF752_13715"
     CDS             complement(11292..>11579)
                     /locus_tag="VF752_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856649.1"
                     /translation="IALALGGAATVLAVGTNWATGIVKVIPPLLHAYVDVIATVALIV
                     LPFAVGFSYRTNALLFYVIVGGGGLLATLATRFPVTRGMSAESAVMFPEAA"
BASE COUNT         1948 a         4025 c         3807 g         1799 t
ORIGIN      
        1 gaccgcgctg cacgactacg ccgagcgcgt gaaaagcgga gccgcacccg gaaaccacgc
       61 ggtcgtggtc gggctgctga gcgcctcgtt gagcgtcgca tgtctcgacg cagtcgtcgg
      121 cgagctgtac gcgttctcgt cgagttgggt cggtgcggcc atgcggctcg gcttggtgga
      181 ccaccggggt gctcaagcgc tgctgcacca ggcgcgcccc gtaatcgcca ccacggcgct
      241 ggaggtgaac gatcgggacg ccagccggat ctccagctgc acccccttcc tggacgtgat
      301 ggcgatgcgt catgagcaag ccgagctccg gctcttcgca agctgagaaa ggacgggttc
      361 ccagtgactg aacccaggca cacgcattcg cacgtccatg acaatgccca tgctccgccg
      421 ctcggcaacg tccgtgcgcc tggcgtcgcg cgaataggaa tcggcggacc agtcggctcc
      481 gggaagactg cgctcatcga ggctctagtc ccgcggctcc tcgtagccgg ccatcgcccg
      541 ctggtcgtga ccaacgacat cttcacccgc gaggacgccg agcacgtccg cgccaccctt
      601 gcggacgtgc tcgatcccca gcgtgtcgtc ggcgtcgaga ccggttcgtg cccgcacacc
      661 gccgtccgcg acgaccccag catgaacctc gacgccatcg aggagttgac cgaccgattt
      721 ccggacgccg acgtcgtgct ggtcgaatcg ggtggcgaca acctgacgct gacgttcagt
      781 tccgcgcttg ccgactactt catcttcgtg atcgacgtgg cggagggcga caagatcccc
      841 cgcaagcgcg gcccgggcgt gaccagctcc gacctgctgg tgatcaacaa gatcgacctg
      901 gccccgtatg tgcgggcgag cttggacgtg atggagcgcg acgcccgcgc ggtccgcggc
      961 ggacggccct tcgtgctcac gaactgcttc accggcgatg gattggccca ggtcatccac
     1021 gaaatctcgc cggtcatgaa tttgtatcgg ccggcgtcgt cgatcagagc cacaccgtga
     1081 cggagccgat cgcgctgagg gaggaccccg agtggtacac gccgtcgacc ctgccggaag
     1141 aggtcatggc ctttgacgac tcgctaaacg gcgcgctcga cgtgggcgcg gccgggaagg
     1201 tcggtctgct cgatctcgcc gtgagccgcc acggcggcgt aacccgtgtg cggcggcact
     1261 accagcgggc gccccttcac ctctatcggc ccatctacct cgacgccggc cgggccgaca
     1321 tggccttcct gttcgtgcag cagttcgggg acggcttggt tcagggagac cgctaccgca
     1381 tcgacgttga ctgcggacct ggctctgcga cgcacatcac cacccaagcc gccaccaacg
     1441 ttttcagggc gcgccagaac ttcgccacgc agctggtcaa cctgcgggtc gccggcggcg
     1501 cggtgttgga gtacttgccc gacccagtcg tgcccttccg cggatcgcgc ctcttccaga
     1561 gaacttgcgt cacggtcgat cccgaggcca cgctgatcct cggtgagact ctgctgcccg
     1621 ggcgcgtcgc ccacgacgag gcgcacgtgt acgacctcta ctggtccgaa accgaggtcc
     1681 gacgcccgga cggatcgctg ctgttcaccg atgtgctgcg cttgcacccg agcgcgggcg
     1741 agagcccgac gtcgatcggt ctgctcggcg cgcacgacgt catagccgct ctctacctcg
     1801 tctcgcggcg aaccgacccg gccgacatgg tcgcgctgct ccgaatcgcg ctcgccgcct
     1861 gccgcgacgt gctcgccgga gtgagcgagc tgccgaaccg ctccggcgcg gcggtcaagt
     1921 tgctcgggcg cagctcgaag gcagtgcata gtgcgctgag gacggcctgg agcgcggggc
     1981 gcctcgaact gctcggcacg ccggcgccga atcttcgcaa gggataactg tcagcgggcg
     2041 ggctcgagga gatcttctgg cttttgccct gccccccgga ggagcgtcag cgctgtcagc
     2101 ggggagagca gcaccgatag gaggccggcg gcgacgagcg ccgcgaacgc cgcgggacga
     2161 atcagatcca gctgtacacc gatctggcca gcgacgacca aggaactgag cgacgtcgcc
     2221 tgtagcagcg cggctgcgag cgtctgtctg cgggtagcaa gcgatttgta gataagcgca
     2281 ggcagcccgc gtgcaacgag caaggctgcg agaaagatcg gcacgcgcgc gagattcgtg
     2341 gcgctcgcga acagcgcgtt cagctcgaac ctcacgccag tcgcgacgaa gaaggctgga
     2401 acgaacacgc cgtacccgac cgcctcgagc ttctggcgga actgcgggtg cgtcagcatc
     2461 tggtcgcgat cgacgagctt gagggtcgca ccggcgagga aggcgccgag aatcgcctcc
     2521 aacccgagac gctcggcgag cacggcgaag accacaagca ggaggaacga cccgcggatg
     2581 cgaatttcgg ctgtcgtgtc ctgcaggcgc agcagcgtcg ccgagatgcg catcgagtgc
     2641 tcggcgccga aaaccgcgat cccgacggcg accacgaaca gtccgaacag cccgagcaac
     2701 agcaacttcg cgctgaagct gctggcctcc cccgagaaga agagcgagag caacacaatg
     2761 ggcgcaatct cggcgatcga ggagcccgcg acgacgagtt gtccaaacgg cgtaccaacc
     2821 tgaccggcgt ccttcaggac cggagtcaca atgcccagcg aggtggcgga cagtgcgatg
     2881 ccaatcagaa gcggcgactt gacgaggccg cttgcgtgga ggccgagacc gattagcagc
     2941 gcaagtccga acgagatcgc gtagccgagc gcggtgagtc ggagcgggcg gccgcgaaag
     3001 cgctcgtact cgacttccag acctgcgagc agaagcagaa aggcgagccc caataggctc
     3061 aggacctgga tcggcgtgtc gatcgaaacc cagcccaacg cttgaggtcc gatcacaatc
     3121 ccgagcacga tctccagcac gatcgccggc aaccggatcc tcgggaagag tccgaggacg
     3181 agcggagccg ccagcgccgc cgccgcgacg atcgctagtc caccgaacga gatcgtgccc
     3241 gccgccgggg ctaccggggg tatccctcgg tcacctcgcc cttgtgtgca gcaaatgcag
     3301 ccagaacctc ccccttctcg ggctcgggca ccttgaagaa gtcaagcgat cgcgcaagct
     3361 cagccgcgac ctcgtcgaac acggcgggag agatcttcag ccggcgatgg gcctcttcca
     3421 gggcaagacg atcatggccc ggcttcgtgg ctgcatattg ctgaggtcca ccagcaatgt
     3481 cgcagaccca cagggtgcgc atgaacttga ggcctggaag cctgcccgga tcgtcggtgc
     3541 tccattcccg tagctcgggg ttctccgatt cctggccggc gattcggtta cgcacgacag
     3601 catcgctgaa gtgctcaatc accgctgcga tggcgaatgc gccaccaagc cgttcgtaca
     3661 gactcgcttg cgtcatttct tacctcctgc tgccttcaga cgccggcttg ctcgagcagc
     3721 gagagccaca gttcgctgcg tgtcgggaag ggcgggacgg cgtggcggag gcgctcgagc
     3781 ggaatctcgc cgaccaccgc gaccgtcgcg gcgtgcagcg ccacaaggcc ttcgacggca
     3841 tgcacgccca atgcacgcac tcgccgccga cgagctgatc ctctaccagc gcaaccgcca
     3901 acccgtgatc tgtgagcctc gccgacgcct cctcgccaga aggcccaccg gcgacgacca
     3961 ccacatcaaa acttgccatg caccacagtc tgtggcccgg ggcgccgcac accagcccag
     4021 ttggcgatga ccgatagtca ctcgcgatgg cgcggctgcg agcgatgttc cgcgacgctc
     4081 actagcgtcc ctcgtcccga ggatgggcca gaaggcggcg gatcgcggtc gccgacagat
     4141 ctgatttggg caaaaaaccg atcgccgggc tcaccgcgat caggtccgcg aagtctgcct
     4201 cgtcgtgtgt tgaaatcagg atcgtgcgcg actccgcgct gtcgaggctt tggacgagac
     4261 ggcgcgccac gtcgaagccg ctttcgcggc cgagatcgat atcaacgagc acgacgtccg
     4321 gcttacgctc ctccaccagc gacacggcct cctggacggt tcgggcaacg ccaacgaccg
     4381 cgacgccctc gcgctcgagg agatcgcttg cggctcgcag cacagctggg ctgtcatcca
     4441 cgattacgca ccgcagcgtc atttcgccga gcatgccgca cgagcaccca gccgcctatt
     4501 accgctagct gcaatttcgc gctagctccg cctgcctcac gggtgtcccg cggcagccat
     4561 tcgcgcccgg agattacgcc tggcgtcgaa gaggttcttg tagaccgcac cgcggttggt
     4621 gccgagcttc agcgccacga tgtcgatcgg gacttcgttg agagcgatcg cgacgaacac
     4681 ctcgcgctgg cgatcggtca gcttcgcgag ggcctgcgac agtgcgtcga gttgcgcacg
     4741 ctgctccatc cggtcctcgg gtcggggcgc cagcggatcg gggagctggt cccaggcgag
     4801 ctcctccacg cccggcggtt gtcggcgcca cgcgtgccgg gcgaccgtgg cagagacctc
     4861 gaagatgacg aacttgtacg cccaggtggt gaatcggctg agcccccgga agtcatcgat
     4921 cctgtccagg actttgacga gtgcgtcgtc ggcggcctgc tgcgcgaggt catcaaactc
     4981 cgggccggtg accgactgca gctgggggcg ccgccgcgac aactcggata tggcgacccg
     5041 ccgcagcaga tcgtgaaggc tgccgacggc ctggtggtgc cgcgggtgcc cgacccgaag
     5101 ttgctcgacc cactgtcgtg aggctgcgtc aggtccggct ggcggcttgc cgagcgcgcc
     5161 cgcgctgggg gggctgggac tcccgttcgc gggccggtca tggatggaac gtgtggtccc
     5221 aatccgatac atgagcgaat ggtccctcca ggccggcgac ttgtctgcac tgccaacccc
     5281 attcgcgcga ccaggctcgc tggaatctat cccttcctgc tacctggaaa gcgaccaccg
     5341 tcctggagcg ctgctcggtg tctcgcgctc gtcggcaacc tgacgtggac agctgttcca
     5401 gcgtccggag agctgtcgat ccggatcgtg cccccgatcg cctcaactcg gtcgaccagg
     5461 ccggtgagcc cggagccgtc cgacgcgtcc gctccgcccc ttccgtcgtc gctgacgatc
     5521 agctcgagcc aaccattgcg ctgccggacc ctgagctcgg cacgtgacgc gtgcgcgtgc
     5581 ttggcggtgt tggccagcgt ctccgagacg acgtaatagg cggccacctc gacgggctcc
     5641 tcgaaccgct gagcgtcgag gtcgaggcgc aggtcgacgg gcaccgcgga ccggcgcgcg
     5701 agcgagcgca gcgccgacct gagtccgccc tcggatagga ccgcgggatg gaggccccgt
     5761 gacagctcgc gcaggccgtc gagaacatcg gcgagccccg tcccgaggtg cgagaggacg
     5821 cgccggagat cgtgcagctc gggcggcacg gtcgtctcag ctgcgcgcag ttcgagcccc
     5881 aacgacacga gccgttgctg cgcgccgtca tggaggtcgc gctcaaaccg ccgacgtgcc
     5941 tcatcccccg ccctcacgat gcgtgctcgc gattgcgcca gatcggagcg gctcttcgca
     6001 ttggccacgg ccatgccggc gacccggctg aactctgcta ggcgcgcctc ggtatcggcc
     6061 ggcagcgggt ccggtctacg ggacgcgacg gcgatcatcc cccaggtcgc cccctcgacg
     6121 acgatcgggg ttgcgacagc agaacggatg ccgatctcgc gcgagagggc agcgatcgcc
     6181 cccggcgcgt cgctgtagtc ctccttgcgc ccgggcaccc cggtgctctt cacttcgcta
     6241 gccaggttct ccccttcgag cgggagccgc tcgcccacgg gaagactgat cgccgcatcg
     6301 ctccaggcgg cgaccaccgt cacggtcgcg tccgcctcat agcgcaggat ctccgaggca
     6361 tccacgccca ggagtactcg cacctcctcg accagcgtcg cgaaaaggtc gtcgggagag
     6421 tgctcgctcg ccaccaacgt cgccacgcgc cgcagtgcgg actgctcggc ggcaagccgc
     6481 tcgaccttcg cgcgggactc gatattcgag atcgccgtgg atacgagctc ggcgaactgc
     6541 gcgacgcggt gttcagtccc gggcggcaag cttctctcgg accccaccgc catcgcgccc
     6601 cataggcgcc cgtcgacaac gatcggaccc gcgaccgcag cgcggacgcc ctgctcgcgc
     6661 acggagtcgc caagcggacc gggtacccgc gaatagtcgt cgacgcgggc cggacgtccg
     6721 gtgcggagca ccttgccgac cacgcttccc tccgggaggg gcacgttggt gccgggggtc
     6781 ggggccatgt acttcggcat gccgtgtccg gcaagggttg tggcggtgcc gtctgcctcg
     6841 aaccgggcag tcaccaccat caggtcggcg cccaggaggc cgctcagctc ctctgtgacg
     6901 agggcgaaca cctgctccgg tggcggttgc gcagcgacca gctcggccac ccgacggagc
     6961 gcggcttgct cgctggcgag ctcgcgggct cgtgcttcgg aggcggcaag gtcagcgcgg
     7021 ctttcggcgt tcgcgatcgc cgtgcctagt agctccgtga acttcgcgag acggctttcg
     7081 aggtcaggcg gcggcggctt cggcccgcgc gagcccacag acattgcccc ccatggagca
     7141 ccatcgacca ctacggggac cgcgaccgga aaaagcgttc ccccaagccg cgcagcttcc
     7201 gcgagcggcc cttgcgcgcc cgccgtgtag tcggcgcgtg ctgcctggcc gctgcggtac
     7261 acacgcgcga cggcgctgtc gtcgccctcg agggcccact gtctgccgac gggcccgagg
     7321 tacttgccgg gcgttgcgag caaggttgcg atgtcgccgg gatcgaagcg catcaggatt
     7381 gtgttcggga tcccgaacat ctgctcgacc tcgtctcgca cggcgtcgaa cacgcggttc
     7441 ggagtggcac cttcggcgac gagcgttgcc acgcgcctca gagcggactg ctcggcggca
     7501 agacgctcca cctgcaatcg cgactcgata ttcgagatcg ccgtggacac aagctcggca
     7561 aactgcgcga cacggtcctc gcttccgggg ggcaacgcct cagccgtctg ggaccccgcg
     7621 accagagcgc cccaaagccg cccgtcaacg acaatgggcc ctccgaccgc gcagcgcacc
     7681 ccctcctcgc gcacgatggc ggagaacgga cccgggacct tcgcatagtc ctcgatgcga
     7741 acgggacggc cggtgcgaaa catcgcttcg agtacgcctc cccccgagag aggcaccttc
     7801 gtgcccgggg ctgtccggtc gtcggtcctc ccgcgttcag ccagtaccgt ggcgctgccg
     7861 tccggctcaa agcggaccgc cccgatcaag tcgatcccca gaaggtggct gagctgctcg
     7921 gttaccgcgg cgaacacccg ctcgggcgcc gcgtgcctgg cgaccagctc ggctacgcgc
     7981 cgcagcgcgg actgttcgtc cgccaaccgt tgggactcgg tctcggcccg ccggcggtcc
     8041 gtcacgtcgc gcgctacccc gtacatgacg ccgtcctcta cgacgggcgc cgccgtccac
     8101 tcgagcaccc tcagagaccc gtctttgcga aagaagcggt tctcgaacga cagcgtcgtc
     8161 tttccctgac cgacgtcggc cgcctcggcc gcggtcttct cgcggtcgtc cgggtggacg
     8221 aagtcgagat atggccgagc gaggagctct tcgtcggtgt agccgagaac ctgctcggcg
     8281 gcagggttga cgcgcgtgag atatccgtcg aagttggcga cggccacgag atccggggag
     8341 aggttgaaga tgcggtcgaa gtcttgctgc gccttggcgc gccgcgcggt attgacagcg
     8401 agcgccgccg cgaaggcggc gacgacgagg ccgccggcga ggaggatcca aggcagaacc
     8461 gcgccggggc cgctgaccga ttccttcggc atgagcaccg cgaactgctg tcccgcgacc
     8521 gtgaacccct cgcgaacggt gtcgccgccg gcgcggtttc ccgacgaacg gccggccggc
     8581 acgaaccgca agcccgcggg gttcctcgcc gccgcgcgca gagtcgcctc ggataggaac
     8641 accaccacgg ccccggggcg gagcacccca tcgatcagat tcggcgcggg cgcgacgagg
     8701 aagaggccgc gcgtcccgtc tctccgggcg gcgataggcg tggcccccac gccacctggc
     8761 tgaatcgcgc tatccagcgc cgcgggaatt ccgagttcac gcctcagatc gatgccgggc
     8821 gagttcatgg gcgggaaacc ggagatcagc gtcgccggca ggtacgagga gcttgtcggc
     8881 acggatttgc gccgctcgtc cggtgcgaca atgcgccggc cgatacgccg ctcgtacgcc
     8941 gagcgatcag cggccccgac ctcctgggcc cacgcagcgg ccgggaggtc ggcggggctg
     9001 agccacctcg atgcgttcct cgtgaactcg gtgttcgtga cgcctctggc accttcgtct
     9061 gacatgaacc ggctcaggct gtcggtgagc gtcgtcgcca cctccacgcg actgcggatc
     9121 tgcgccgccg cgatctcgat acgccgctca gagccgtgct tcgcatcccg ctcggcgagt
     9181 acgcgggcga cgatgaaacc cgcgaccgtc agcccgagaa ccagtgccac cgctgcgatc
     9241 tgtcgcggcc gcaagagcga acctccgcgt tcttagcgat tgtgaccctc cggaatcgcc
     9301 gggacaaggc gggctcgacc agagggcgga gctctcaagt gatcctgtgc cagccggcga
     9361 tcttcgccgc gaggtcgtcc atcgccgccg tgtatgcctg gtaggacggc ggcgtgtcca
     9421 ccaaccacct gccgagctgg ttcgcccgca ccgccggcag tgctgcgaag gccgggatct
     9481 gcttcgcctg cctgagcggc agaaagagtg accgcgcgtc gtagaggatc ccgtccgccg
     9541 ggtacttggc ggcatggtcc caactgaacc ggtcccagta ggcctccgat gtctccgccg
     9601 agatgagtgg cacgccgcgg ctcctgtaga aagcgagctc gcctgagtcg accaccttcg
     9661 caacgtacat cgtgctctgg tcccccgaga tggcgatgat ccgcaaattc gatttgcgcc
     9721 ttgcgatctg ggacaagctc gctgcggctc tgagaaacgc cgcgcgcgcc cgcccattcg
     9781 cgccccccat cgtgtccgaa ccgagctcac ggacgagatc gccgaagcga tcgatctgct
     9841 tcgtcgcgat cacatggccg ctgaacccga cgattggcac ccgcgagccg atccgcttct
     9901 gctgcgcgag gtccttgaac ccgaagagcg gtgagtcggc gggcggcggg taccaccgcg
     9961 agacgatcaa ctcggccttt agcaaggcca gcttcgcggc atctatctcg ccgtagacgg
    10021 agccgatgat ctccgactcg gcccatggga acccagcgag taccggatct tcttccgggt
    10081 tgtcgccgaa cacgccgaca ggcgtcacgc cccactggtg aagcgcggcg gccgccgtgg
    10141 tgtaggcgac gatccgcgtc gggcgcttgc tcaacctcac cgtttgcttc cgatcgtcga
    10201 cgaaggtcca gcctgccgca tccttatccg ctgagccgca gcccactacg acgctggcgc
    10261 ctgctgcaaa ggcggcggcg agcacctgcc tccgtgtgag cggccactcg agggcgttcc
    10321 cgttcatcgg cttcctgcga ccacagagta cgccgacagg cgagcccgcc ttatggatcc
    10381 cgcgccccgt tcttcgcaac cgagaacggc aagccgcgtt attgcggttc gtttgagctg
    10441 atcggcaccc ggggcgcggt cggcggctgc gggagatcgt agaagcgccc agcgctcttg
    10501 ccctgctccc ccaatccgaa cccggcgctc atgagcgcgg tcttgatctg actgatgcga
    10561 tcaaactcga acactgcgaa tccgagatca ttgggcgggc ttgcgccggc cagcgtttgc
    10621 ccaagcacgc ggtgctcgga ctcgaccgcc gccagctccg ccgagtagcg cgcgagctcc
    10681 gggcgcccgg cggccgcaca cgtagtgacg ccgaccagat atgtgttgac gaaaaggtgc
    10741 tcgccagccg cgaccccctg gccgaccgca gttaggtcga gagcatcgcc cgcgccgccg
    10801 aagaaggcgt ccggaatcca gaaccgcgtc gtggtcgggc ggtagagctt gcgtatgaac
    10861 tgccaatggt cccgctcggc ggcgtagacg ccgtcaaaga tgaccggcac cgagctcggc
    10921 acctcgggat gcaggctcgt gcgccgtacc agctcagtca ggatcgtgac ggttaacgcc
    10981 tcggtcgtgg acgcgaagac accgaactcc tggaccgaat cgagcacgtc gaccgagcgc
    11041 ccggcgcccg ggccggtcgg gacaggttct gtagaggaca tgcgagtgcc tttctgtatt
    11101 gggtgcgcgc agctcaggcg ctacgcggtt cagctcatgc gcacccaacg atgcgtcctg
    11161 cgccttcgtc caacaacacc tttatgcgaa cggcgatagg cctgagcgat cagagcccgg
    11221 cgggtgatgg acgcggcgac ctcgccgtcc cgccacctac gacgagcgtg gcgggacggc
    11281 atcgcagcgc gctacgccgc ctcagggaac atcaccgcgc tctcggccga catgccgcgc
    11341 gtcaccggaa agcgtgtcgc gagggtcgcc agcaaaccgc ctccgccgac gattacgtag
    11401 aagagaagcg cgttcgtgcg gtatgagaag ccgacggcga atggcagcac gatcagagcg
    11461 actgtcgcga tgacatcgac gtatgcgtgc agcagcggtg ggatgacctt cacgatcccg
    11521 gttgcccagt tcgttcccac cgcgagcacc gtggcggcgc cgccgagcgc gagggcgat
//
LOCUS       DASVXS010000242        24344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_422823, whole genome shotgun sequence.
ACCESSION   DASVXS010000242 DASVXS010000000
VERSION     DASVXS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24344
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422823"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VF752_13720"
     CDS             complement(<1..182)
                     /locus_tag="VF752_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856650.1"
                     /translation="MSGTGLTNGASRYQTEKVRVAIVGVGNCASAFVQGVQYYKDADP
                     AKSVPGLMHVDLGGYHV"
     gene            complement(258..932)
                     /locus_tag="VF752_13725"
     CDS             complement(258..932)
                     /locus_tag="VF752_13725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8856651.1"
                     /translation="MSLDPGRDAPPRTRGGRQRTRRDGGEPLTVAEVRTLARNRLIES
                     RLTPNAISLTGLVLNIAAAVLVTQHYWFLAGITFIVGSIMDTLDGRYSRMSGKGTPFG
                     AFLDSTLDRMEEAIVLTAVAAYFASNGQDFATAATAWTVGASLLVSYTRARAEALGVE
                     CKVGLADRATRVVILSAGLLFAKGAGLGDFSLLKPAIYVMAVLTTITVLQRVLHVRRE
                     LNRAAA"
     gene            complement(929..1687)
                     /locus_tag="VF752_13730"
     CDS             complement(929..1687)
                     /locus_tag="VF752_13730"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8856652.1"
                     /translation="MSRQWLPQGWLDALRQLLIFAIAYYAYRLVRGAVDGQVASAFAH
                     ARDVIAIERSVHLFFEAQLQDWVNRVGWLEDVADFMYVNSQFVVTTTFIVWLYLARNE
                     SFYYVRNMFAVAMVLALIGYIVFPTAPPRYMPEWGFHDSVSDFVGDGAANSAAALYNP
                     FAAVPSMHVAFALMVGIPCAVLVRSRALKVLWCLYPLLISFVVIATANHFWFDAVTGA
                     LVAGMSAATAHAALARARPDAWSFRRPLGAEAPL"
     gene            complement(1750..3333)
                     /locus_tag="VF752_13735"
     CDS             complement(1750..3333)
                     /locus_tag="VF752_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856653.1"
                     /translation="MIVTALGAAVYVAVHPPVGDMAAHVFRTDLFGAHGLAIWNDQWY
                     GGHHVPAYSVLFPPLAWLLGPFAVGALSAVGATAGFEAIVHQHFGERSRYGALWFGVA
                     VLTLLLTGRLPFAFGVAIGLAALLALQRRRRALAIALAAFTPLASPVAGAFLGMAGAA
                     HALQGRRERSEAWTEGLAVAAAALLPPLILSAAFPEGGHQPFAVTAYLPAPLAALGFL
                     VLLPRRESTLRIAAMLYLISATAAFVLATPMGSNTARLGALFAGPLAACVLAGAPPGR
                     SRTAAIVALTALLVPLAYWQWSPAVRAVSETVGDHSRNPAYYAPLLRFLDRHREDAPA
                     RVEIVFTEGHYEAADVARRFPLARGWERQVDVGRNDIFYSRQKLTPDRYEHWLSSQAV
                     RWVAVPDAKLDYSSVHERALINSGLPYLKLVWRNKHWRVYEFTGPHPFVIPDGVRRGS
                     PARIQAVDVGPAHVDLRVRRPGSAVVRVRWTPYWRAHKACVEPAGNWTRVIADRRGWV
                     RLNVSFAVSRVFSRGLRCG"
     gene            complement(3479..4309)
                     /locus_tag="VF752_13740"
     CDS             complement(3479..4309)
                     /locus_tag="VF752_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856654.1"
                     /translation="MQTTVQEKQGQIAGLDTFWREAPPREGHAPVLYVHGVPTSSDDW
                     VDFLERTGGLAPDLPGFGRSAKPAQFDYSIDGYSDWLDAFLADRGAERYSLVVHDWGG
                     AALGLAQRAPERLERLVIMNAAPLLPGYSWHRIARVWRTPLAGELFMGLSTRWGFRQL
                     SREATVAPGPASDEFIDRIWKYFDHGTQRAILKLYRSAPADSLAAAGQRLGEIRAPAL
                     ILWGAQDPYIDAPRFARAYAETLGGETRLELLEDASHWPWIDRPDVVETVSSFLLETG
                     "
     gene            4357..5064
                     /gene="nth"
                     /locus_tag="VF752_13745"
     CDS             4357..5064
                     /gene="nth"
                     /locus_tag="VF752_13745"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX8856655.1"
                     /translation="MTATKKSDWKPPTKRKLIAIRDRLRKAYGKPEPRPHRAPIDELI
                     LTVLSQNTNDRNRDVAYGRLRQRFPRRDGSPLPDWDAVGNAPEDAVEEAIRPGGISRV
                     KARRIQEILRSLGDDDLTHLETDPLDEARDYLCSLPGVGRKTAACVLLFSYGRPDVPV
                     DTHVYRVGTRLGLFRPGASFDEAHDEMLRLTDPEDAYEIHVGLIRHGRRICTARNPRC
                     RECPLLSLCPYGKARVR"
     gene            5061..5939
                     /locus_tag="VF752_13750"
     CDS             5061..5939
                     /locus_tag="VF752_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8856656.1"
                     /translation="MTEQHRAEAALVGIAAVWGLTFVMVQDAIEQLPSLAFLAYRFVP
                     AGLIVALFARRALRGLGRAGWLAGLAMGVFLTAGYIFQTIGLEHTSASNAGFITGLFV
                     VFTPVLAAVFLRERIGALAWGAAALSALGLYLLAGATHGFNEGDLLVLGCAVAFAAHI
                     IVTARGVRAHDVGALLTIQLTFCGVVCLIAAAAAGDLEVPRGGTVWSALVVTSLVASA
                     LGFFVQSYAQQHAPPARTALIMASEPAFAGFFGWAIGGETLSLVSWFGALLIMAAILA
                     VEVVPRFRPPRPLPEG"
     gene            5972..6250
                     /locus_tag="VF752_13755"
     CDS             5972..6250
                     /locus_tag="VF752_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856657.1"
                     /translation="MRDLARFAAMGLYLFDLFLLRLVGAGAMAVSSTGAVLALRMTPG
                     ILSIAAVGVVASGLWSYGALQRPVPARVKPATWFAAGSMLVWFLLLLV"
     gene            6449..8362
                     /gene="dnaK"
                     /locus_tag="VF752_13760"
     CDS             6449..8362
                     /gene="dnaK"
                     /locus_tag="VF752_13760"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8856658.1"
                     /translation="MGKTIGIDLGTTNSAMAVLEGGEPTVVENAEGGRTTPSVVAFTA
                     GGERLVGTVAKRQAVTNPENTVFSIKRFMGRKEAEVREEESIVPYKVEAGPNGDVRVD
                     AGGKQYSPPEISAMILQKLKADAEAYLGDTVDSAVITVPAYFNDDQRQATKDAGKVAG
                     LDVKRIINEPTAAALAYGLDKEESDHTILVFDLGGGTFDVSVLEIGDGVFEVKATAGD
                     NHLGGDNFDKAIVDWMVKEFKASQGIDLSQDRMALQRLYEAAEKAKVELSSTQETQIN
                     LPFVTADQSGPKHLDMRLTRSKLNELTSDLLDRTVGPTKQALQDAGITPGDIAHVVLV
                     GGMTRMPAVQEKVKELTGKDPHRGVNPDEVVAVGAAIQAGVLAGDVKDVLLLDVTPLT
                     LGIETKGGVMTKLIERNTTIPTRKSEVFSTAEDNQPSVEVHVLQGEREMAIGNKSLGK
                     FQLTGIPPAPRGVPQIEVTFDIDANGILNVSAKDLGTSKEQKIEIKAGSGLSEDEIKR
                     MVTDAEAHAEEDRRQRELAEARNTGENAAYQAERQLADMGEQVDSSAKEQIDAAIKDV
                     RDSLTSDNADEIRQKTASLQEAFHRVSEQMYAQAAQAQQPQGDGAANGATTDSSDEEE
                     VVDAEVVDGDSKS"
     gene            8359..8964
                     /locus_tag="VF752_13765"
     CDS             8359..8964
                     /locus_tag="VF752_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX8856659.1"
                     /translation="MSIHEQQRQDERERLNDEPLPTQQPEEGEPAGADDESAEQELEE
                     ATERPVDEGGDDLTRAQRERDEYLSLAQRTQADFENYRKRAAKEAAGAGERAKAGLVR
                     ELLPVLDNLERALASANSPDDVVAQGVKLVHADLVGVLQRAGVEAFDPAGEAFDPTVH
                     EALTTRPAEDGVHPGTVLDVVEKGYRLNDTVLRPARVVVSA"
     gene            8967..10082
                     /gene="dnaJ"
                     /locus_tag="VF752_13770"
     CDS             8967..10082
                     /gene="dnaJ"
                     /locus_tag="VF752_13770"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8856660.1"
                     /translation="MAAVRDPYKTLGVDKKASQDEIKKAYRKLARQYHPDQNPEDPHA
                     EERFKEVQEAYSVLSDPEKRKQYDQGGGIFGGFGAGGFDPGSFRTGGGGGGFGGIGDI
                     LSDLFGGGGAGGPRAQQPERGRDLETEVHISFDQAMKGAQVPIVATIPSPCPTCHGTG
                     ARPGTSPTVCPRCQGRGIESQGQGLFSISQPCSLCGGTGTQISDPCPTCHGRGQTPQT
                     KRYRANIPAGVRDGSRVRLAGKGEAGRRGGPPGDLYVVTRVEESSIFKRKGDNLEVDV
                     PITIVEAIRGATVEVPTLNGSKRIRVPAGTQHGTVQRLRGEGPARLNGKGRGDIHYRL
                     VIDVPRSLSKQQEDAVDGLAEVLNGNPRERLFAGRDR"
     gene            10082..10543
                     /locus_tag="VF752_13775"
     CDS             10082..10543
                     /locus_tag="VF752_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8856661.1"
                     /translation="MARRKQLDPRQGVYMISVAAQLAGMHPQTLRIYESRGLITPKRS
                     QGNTRLYSQTDVERLRRIQELTSEMGMNLAGVERVFELEEQLERMQDKMLKLEREAEQ
                     MQEELKLEIERVQRSFKFELVPYEEPGHALIPAKEAAKGVRIPITRRDGAR"
     gene            10546..10944
                     /locus_tag="VF752_13780"
     CDS             10546..10944
                     /locus_tag="VF752_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856662.1"
                     /translation="MLGDAQLLTRVYDAFNTGGVRAVADYIHPDAEFIDPVEFPGADH
                     YMGREAVISYLENFVEAWGVVNIVIEEFHGAGGRVGAQLCIELRGAASGYETEMRVMH
                     VIDIEDGQVRRVQGFFDREEGLAALVGKPA"
     gene            10971..13505
                     /gene="clpB"
                     /locus_tag="VF752_13785"
     CDS             10971..13505
                     /gene="clpB"
                     /locus_tag="VF752_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX8856663.1"
                     /translation="MNVDRFTIKSQEALQAAQRLAGARRNPQVAPQHLFAALLEQEGS
                     IVVPVLRRAGADVEGVRRRNNEKLDGFPTVSGDATQAPSLSQATIDTLQKADEEARGF
                     GDEYVSTEHLLLALASDPNIDAGATRDQLADAIQAVRGPHRVTDQNPEDKYQALEKFG
                     RDLTAAAEEGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEGLAQRI
                     VSGDVPESLRDRRVIALDIGALIAGSKYRGEFEDRLKAVLKEVQEAEGRVILFMDELH
                     TIVGAGAAEGAVDAANLLKPMLARGELRAVGATTLDEYRKHIEKDAALERRFQPVMID
                     EPSVEDAIAILRGLKERYEVHHNVRIQDSAIVAAALLSQRYIADRFLPDKAIDLIDEA
                     ASRVRIEIDSVPTEIDEVERRVMQLEIEQQALSKEEDEASVARREAIERELADLREQT
                     NAMKAEWQREKGAVDAIADLKKRLDEAHREVERAERDADLEQAAKLRYGEIPELEKAL
                     EEAEHTVEGERKFFKQEVDDEDVAEVVAKWTGIPVSKLMEGEVEKLVHMEDRLHERVV
                     GQDDAVEAVSNALRRSRAGLQDPNRPIGTFLFLGPTGVGKTELARALAEFMFDSEQAM
                     VRIDMSEYMEKHAVSRLVGAPPGYVGYDEGGQLTEAVRRRPYSVVLLDEIEKAHPDVF
                     NVLLQVMDDGRLTDGQGRTVDFKNTVLIMTSNVPGGMAGVEATFKPEFINRLDEIVQF
                     HQLTREEIGSIVEMQVAKLSARLAEKGIEIELTDDATMLIGNLGYDPTYGARPLKRVI
                     QKQLVDKLALALLEGEFAAGDRVLVDAEDGELKFSKAGAVAPAAVA"
     gene            complement(13533..14474)
                     /locus_tag="VF752_13790"
     CDS             complement(13533..14474)
                     /locus_tag="VF752_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX8856664.1"
                     /translation="MRKISRATLGAIAALLVFAASANASIIFPDGDITKETGPSGLAV
                     PVSYTLPTTSTEGPLIGPATCTPVPGATFVVGTTPVSCIGLVMQPCSVIVDPTCIGGF
                     KFLSPNGTFTVTVTPGQGPALPVSDKTVETNGDNAVIFYDLPAATDPSGVEPGSVACT
                     PGSGSTFPVGATKVTCTAKDTVGNASSAAFTVNVVKKATSSGTATPMPVAFTAGNGTV
                     SGNYAYVPLGCPAASSVDCAGTLTLTTSSNAAKTIKAGTAKFKIAKGKSAKVKVKLTK
                     QARKILARKKKLRVKAKATTGSLQSTRSLTLKLAKKK"
     gene            complement(14590..15519)
                     /locus_tag="VF752_13795"
     CDS             complement(14590..15519)
                     /locus_tag="VF752_13795"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8856665.1"
                     /translation="MTRFVAADRQQPAGEAGPQGGLEPVVRQNIGERIERLASLDALA
                     DPLQKGVRAAVPEDSKLKDLLSGTWLGHPLHPPLTDVVVGAWTSALLLDLFGGDGAES
                     SADGLVGVGVLAAVPTATAGLSDAAELRGGPRRVAIVHATGNVTAIVLHTFSWAARRR
                     GERGRGIALSALGYGFATFSALLGGHLSFGLGVGVNETAFEDAPSDWTPVIEEGALEE
                     GKLIGARANGLGVLLVRNHGRIHALADRCSHRGCSLHEGRLNDDNTVTCPCHGSTFRL
                     DGSVVKGPATGPQPTFAIRTNQGTVELKQAEAA"
     gene            15625..16275
                     /locus_tag="VF752_13800"
     CDS             15625..16275
                     /locus_tag="VF752_13800"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX8856666.1"
                     /translation="MGGSVAVVDPDEQIAARGEDGRRRCRWALGTPDYLAYHDEEWGR
                     PVTGEDELFERLSLEAFQSGLSWLTILRKRGNFRRAFAGFSIECIAAFGPADFDRLMN
                     DAGIVRNRLKVEAVMANAHAAAEMHARGELLSELFWSHRPRGRQRAPRTWGDVPATTP
                     ESEALAKELKRRGFRFVGPTTVYASMQACGVVNDHAAGCWVRPEVERERRQSLEQT"
     gene            complement(16254..17321)
                     /locus_tag="VF752_13805"
     CDS             complement(16254..17321)
                     /locus_tag="VF752_13805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8856667.1"
                     /translation="MATEQIDTTRAEEFAGQMIGILNGGMTSLLVSIGHRLGLFDSLA
                     ELPPSTSAEIAEATGLDERYIREWANGLTVAGVLVHDSESNTYALPPEHAAAITRAAG
                     PDNVATLAQYVGLLAEVEEPVIQCFRNGGGVPYSAYPRFQALQAEETAQVYDAALVHG
                     VVPLVPGLTERLEQGIDAIDVGCGQGHAVNVLARAFPNSRFTGRDISEEGIAAGREEA
                     NALGLTNARFEQASVTDIEGEYDLITAFDVIHDLADPAGALAAISRSLKPGGTYLMVD
                     IAGSSDVDRNADHPLGPALYTVSLMHCMTVSLAQHGAGLGTMWGEELAQQMLADAGFA
                     KVSVQRVDGDFFNVYYVCSKD"
     gene            complement(17411..17566)
                     /locus_tag="VF752_13810"
     CDS             complement(17411..17566)
                     /locus_tag="VF752_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856668.1"
                     /translation="MEALAVLARERGCYGMWTLTDHDNEAGQATYESAGAQISSSHPM
                     PEWDFRR"
     gene            17647..17871
                     /locus_tag="VF752_13815"
     CDS             17647..17871
                     /locus_tag="VF752_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856669.1"
                     /translation="MRQLHAGHESDWLIGVVVGDQQVVVGVLEKAPRRVFQWSAVEER
                     AGSGQRVLVAGAETADLDVHHEMRAARDSA"
     gene            complement(17838..19448)
                     /locus_tag="VF752_13820"
     CDS             complement(17838..19448)
                     /locus_tag="VF752_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8856670.1"
                     /translation="MRRGDWSEARREFEAAVAEEPSVAAFDRVGEACRRLYDSRAAAE
                     AYQAGYSLARENDDSRGAAWMALWIANCAYDMGQGTAVALGWLERARALLDGAGDVRE
                     LAWVEGFEAHILLLRGGDNARALELARSAHQIGRENGDLDIQMLGNALEGLILVSDGD
                     VAQGMRRLDEATAAALAGELRSVDAIATVCCYLIDACKRVRDFERAEQWCARVTEMSE
                     RWSDQLLFAICRTHYADVLTWRGDWAEAESELDQVVRAVGATPGAHEHDAVARIGELR
                     RRQGRLDEAERLLAQADSHAMGALGLAWLALDRGDPAAAATRAARFLRRVSPLDRTQR
                     VPGLEVLVRSRLALGEMTGAGEAARELRAIADAIGTDPLAAAAALSEGLVAVASGEDE
                     RGRAFLEDAVDLYERSGSPYEAGRARLELAAALGTGAEAAAEARRARETLLHVGARLD
                     AERAAALARAAAESPSDPAGLSRREVEVLRLLADGLSNEEIAAALVLSVRTVERHVSN
                     IYLKLGVSGPSARASATAYALSRAARIS"
     gene            complement(19549..20190)
                     /locus_tag="VF752_13825"
     CDS             complement(19549..20190)
                     /locus_tag="VF752_13825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8856671.1"
                     /translation="MPSKLAENTWGRVFAAVYDRLQKGAEEAGVREMRRGLVSQASGR
                     VLEVGAGTGLNLDHYGDVEELVLAEPSPHMASKLEHKVAASGRAAQVVQAPAERLPFP
                     DNTFDTVVVTLVLCTVPDQSDALTEIHRVLKPGGRMLFMEHVRSEDAKIARWQDRVER
                     PWGWFACGCHPNRDTLATIQRSPLELEFSAREKAPKVPRLVNEGVAGAARKPA"
     gene            20293..20574
                     /locus_tag="VF752_13830"
     CDS             20293..20574
                     /locus_tag="VF752_13830"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX8856672.1"
                     /translation="MPTFVLLSTLTPEGVQTVKNNPTRIKEVNKEVEQLGAKVVAQWA
                     TLGRFDFVNIVEAPDEQTIARVSLELGSRGTSKYESLVAIPVDDFIAAL"
     gene            20576..21853
                     /gene="purD"
                     /locus_tag="VF752_13835"
     CDS             20576..21853
                     /gene="purD"
                     /locus_tag="VF752_13835"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX8856673.1"
                     /translation="MKVLVVGGGGREHALVRALMRSPQQPEILAAPGNAGIARDGIRL
                     LPVGAEDVSALVAAAREERVDLVVVGPEAPLVAGLVDVLANSGVRAFGPSAAAARIEG
                     SKTYSKELMRQVGVPTAEYAVLKSREEAEEHIAGAAYPLVLKADVLAAGKGVIICTDE
                     QEARAALDEFFLQERFGRTTVVAEEFLEGEELSLLALCDGIRAVPMAPARDFKRIFEG
                     DQGPNTGGMGSFSPVPGIDSDHARALARAVHQPIVDELRSRGTPYHGVLYAGLMMTAE
                     GPKVLEYNARFGDPETQAILPRLQSDVLELFDRASRPGGLEGVELEWSPSWAVTVVLA
                     SRGYPESSSKGDVITGLEQVASLDAEVLHAGTAEENGRIVTAGGRVLNVTGMGPTVAE
                     ARARAYAAADKIEFDGKQLRRDIAARVAEEVNA"
     gene            21850..22332
                     /gene="purE"
                     /locus_tag="VF752_13840"
     CDS             21850..22332
                     /gene="purE"
                     /locus_tag="VF752_13840"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX8856674.1"
                     /translation="MTEETSGAPAPATETAVESAFDELDVDAPLVGIVMGSKSDMPVM
                     EKAGNELEERGIRYEIRVMSAHRDPEVVADYAVNARMRGLKVIIAGAGLAAALPGVVA
                     AHTDLPVIGVPLTSATSVGGGLDALLAIAQMPPGVPVACMGVNGAKNAAVLAAKILDA
                     "
     gene            22329..23675
                     /gene="purB"
                     /locus_tag="VF752_13845"
     CDS             22329..23675
                     /gene="purB"
                     /locus_tag="VF752_13845"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX8856675.1"
                     /translation="MIERYTRPEIGAVWSEHRKLETWLAVELAAVEVLGEQGVIPPGD
                     VDQIRDRASFTVEAVQEREKITDHDVAAFVDVVAESVGGDAGRWVHHGLTSSDVLDTA
                     LGLQISRAGMICIGGACDFRDALIERARRHVHTLCVGRTHGVHAEPTTFGVKLAGFAF
                     EAHRNVLRLERAVEQASVGALSGAVGTYASNGPEFEVRVLERLGLAREDVSTQVVARD
                     RHAQLLSAIAIAGAGLERFATEIRHLQRTEVREVEEPFRAGAQKGSSAMPHKRNPIVS
                     ERITGIARLLRGYAQAATEDVALWHERDISHSSVERVNLPDATILLDYAQHLATRVVN
                     GMVVHEDRMRENLNLTHGALFSQRALLALVEAGRTRDEAYRIVQKDAQRAWEEGIEFR
                     ELLAQDAPDLDLDAIFDPSAFVRYADEIVGRLDRIAPRSLDTAEARADDRVGPSVP"
     gene            23794..>24344
                     /locus_tag="VF752_13850"
     CDS             23794..>24344
                     /locus_tag="VF752_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX8856676.1"
                     /translation="MTQAAPLAGYDLVAKGKVREIYDAGDDRLLLVASDRISTYDVVH
                     PTPIPGKGQVLTGLSVFWFERTADIVPNHLISYEDVPDEVRGRALLVKKLDMAPVECV
                     ARGYITGSGWKDYLATGAVCGIELPAGLQESERLPEPIFTPATKADVGDHDENVDFDR
                     AAEILGDRPLLEELRRLTLEVYS"
BASE COUNT         3860 a         8394 c         8572 g         3518 t
ORIGIN      
        1 acgtggtagc cgcctaggtc cacgtgcatc aggcccggca cgctcttggc cgggtctgcg
       61 tccttgtagt actgcacgcc ctgcacgaac gcagaggcgc agttgccgac gccaacgatc
      121 gccacccgga ccttctcggt ctggtagcgc gaagcgccgt ttgttagtcc ggttccgctc
      181 aagtactgcc tcctgtttcc atggccgcca aaccccgggt ggcgggccgc taaatgaact
      241 ccgcgccggc tgatcggtta cgcggcggcc cggttcagct cccgccgcac gtgaaggacg
      301 cgctggagca ccgtgatggt cgtgaggacc gccatgacgt atatggccgg cttgagcagc
      361 gagaagtctc ccagaccagc gcctttcgcg aagagcaacc ctgctgacag gatcacgacg
      421 cgcgtcgcgc ggtccgccag acctaccttg cattccacgc ccagagcctc ggcacgggca
      481 cgcgtgtagc tcaccagcag cgacgcaccg acggtccacg ccgtcgccgc agtcgcgaag
      541 tcctgcccgt tgctcgcgaa gtacgcggcg accgcggtca gcacgatcgc ctcctccatg
      601 cggtcgaggg tggagtcgag gaatgccccg aagggcgtgc ccttgcccga catgcgcgag
      661 tagcgcccgt cgagcgtgtc catgatcgag ccgacgatga aggtgatgcc ggcgaggaac
      721 cagtagtgct gggtcacgag caccgccgcg gcgatgttca gcacgagccc cgtgagcgag
      781 atcgcattgg gcgtgaggcg cgactcgatc agccggttgc gggcgagcgt gcgcacctcg
      841 gcgacggtga gcggctcgcc gccgtcacgg cgcgtgcgtt ggcgcccacc acgcgtgcgc
      901 gggggcgcgt cgcgacctgg atcgaggctc acagcggtgc ctcagccccg agcggccgcc
      961 ggaagctcca agcgtccggg cgggcacgcg cgagcgcggc gtgcgccgtg gcggccgaca
     1021 tgccggccac gagcgcgccg gtgacggcgt cgaaccagaa gtggttggcg gtggcgatca
     1081 cgacgaacga gatgagcagc ggatacaggc accacagcac cttcagcgcg cgcgagcgca
     1141 ccaacacggc acatgggatg ccgaccatga gcgcgaacgc gacatgcatg ctgggcacag
     1201 ctgcaaaggg gttgtacagg gccgcggcgc tgtttgcggc gccgtccccc acgaagtcag
     1261 agaccgagtc gtggaaaccc cactcgggca tgtaccgcgg cggcgccgtc gggaacacga
     1321 tgtagccgat cagcgcgagc accatcgcca cggcgaacat gttgcggacg tagtagaagg
     1381 actcgttccg cgccaggtag agccagacga tgaaggtggt cgtgacgacg aactgcgagt
     1441 tcacgtacat gaagtccgcc acgtcctcga gccagccgac gcggttgacc cagtcctgca
     1501 gctgcgcctc gaagaacagg tgcacgctgc gctcgatcgc gatcacgtcg cgcgcgtgcg
     1561 cgaaggcgga ggccacctgg ccgtcgaccg cgccgcgcac gaggcggtag gcgtagtacg
     1621 cgatcgcgaa gatcagcagc tgcctgagcg cgtcaagcca gccttggggg agccactgtc
     1681 ggctcatatc gaggacgcga cgagccaccg gtggagctta gccagccgcc ttagtcggca
     1741 ccagccgggc tacccacacc tcagcccgcg cgagaagacg cgggacaccg cgaagctcac
     1801 gttcaggcgc acccagccac gccggtcggc gatcacgcgc gtccagttgc cggccggctc
     1861 gacgcaggct ttgtgcgcgc gccagtacgg cgtccagcgg acccgcacga cggcggagcc
     1921 ggggcggcgt acccggaggt ccacgtgcgc gggaccgacg tccaccgcct ggatccgtgc
     1981 ggggcttccg cggcgtacgc cgtcggggat cacgaacgga tgcgggcccg tgaactcgta
     2041 cacacgccag tgcttgttgc gccacacgag cttgagatac ggcagccccg agttgatcag
     2101 tgcgcgctcg tgcaccgagc tgtagtcgag cttcgcgtcg ggcaccgcga cccagcggac
     2161 cgcctggctc gagagccagt gctcgtagcg atccggggtg agcttctggc gcgagtagaa
     2221 gatgtcgttg cggccgacat cgacctggcg ctcccagccg cgcgcgagcg ggaaccgccg
     2281 cgccacgtcg gcggcctcgt agtgcccctc ggtgaagacg atctccacgc gcgccggtgc
     2341 gtcctcgcga tggcggtcga ggaagcgcag gagcggcgcg tagtaggcgg ggtttcgcga
     2401 gtggtcgccg acggtctcgc tgacagcgcg gacggcgggc gaccattgcc agtaggcaag
     2461 cggcacgagc agcgcggtga gcgccacgat cgcggcggtg cgcgagcgcc cgggcggcgc
     2521 gccggctagc acgcaggcgg ccagcgggcc cgcgaacagg gcgccgaggc gggcggtgtt
     2581 gctgcccatg ggtgtcgcca acacgaaggc cgcggtcgcg gagatcagat agagcatcgc
     2641 ggcgatccgc aacgtcgact cgcggcgtgg cagcagcacg aggaagccga gcgcggcgag
     2701 cggggccggc aggtacgccg tcacggcgaa cggctgatgc ccaccctccg ggaaggcggc
     2761 cgagaggatc agcggcggca gcagcgcggc ggcagccacg gcgagcccct ccgtccaggc
     2821 ctcggaccgc tcgcggcggc cctggagcgc gtgggcggct cccgccatgc cgaggaaggc
     2881 gcccgcgacc gggctcgcga gcggcgtgaa cgcggccagt gcgatcgcga gggcacgccg
     2941 ccgccgctgc agcgccagca gggcggcaag cccgatcgcc acgccgaacg cgaatggcag
     3001 gcggccggtc agcagcagcg tgagcaccgc cactccgaac cagagagcgc cgtagcgact
     3061 gcgctcgccg aagtgctggt gcacgattgc ctcgaacccg gctgttgcgc cgacggccga
     3121 cagcgctccc acggcaaacg gcccgagcag ccaggcgagc ggcgggaaca gcacgctgta
     3181 ggcgggcacg tgatggccgc cgtaccactg gtcgttccag atcgcgaggc cgtgcgcgcc
     3241 gaacaggtcg gtgcggaaga cgtgcgccgc catgtcgccg acgggcgggt gcactgccac
     3301 atagacggcc gcgccgagcg cggtgacgat cactggcgca agccgagcgg gaccacgcgc
     3361 cgccagtcga gccacagctc cgcgtgcccg agcggagcgg ctttcgaacg cggcgtccgc
     3421 ggctccgccc gaactgctgg acgggagtgg cggcggcacg gctctgaaga gtactgctct
     3481 agcccgtttc caggaggaaa ctcgagaccg tctcgaccac gtccggccga tcgatccacg
     3541 gccaatgact cgcgtcctcg agcagctcga gacgggtctc accgccaagg gtttccgcgt
     3601 acgcccgggc gaagcgcggc gcgtcgatgt aggggtcctg ggcaccccag aggatcagcg
     3661 ccggcgcgcg tatctcaccg agccgctggc ccgccgcggc gagcgaatcg gcgggagccg
     3721 agcggtagag cttcaggatc gcccgctggg tgccgtggtc gaagtacttc cagatgcggt
     3781 cgatgaactc gtcggatgcg gggcccggtg ccaccgtcgc ctcgcgcgac agttgcctga
     3841 aaccccagcg cgtggagagc cccatgaaga gctcgcctgc cagcggcgtg cgccagacgc
     3901 gcgcgatgcg gtgccagctg tacccgggga gcagtggcgc ggcgttcatg atcacgagcc
     3961 gctcgagccg ctcgggggca cgctgcgcga gtccgagcgc ggcgccgccc cagtcgtgca
     4021 ccacgagcga atagcgctcc gcgccgcggt cggcgaggaa ggcgtcgagc cagtcgctgt
     4081 agccgtcgat cgaatagtcg aattgagccg gcttcgccga gcggccgaag ccgggaaggt
     4141 ccggggcgag gccaccggtg cgctcgagga aatccaccca gtcgtcggag ctcgtcggca
     4201 cgccgtgcac gtagagcacc ggcgcgtgtc cctcgcgcgg cggcgcctcg cgccagaacg
     4261 tgtcgagtcc ggcaatctgg ccctgcttct cctgcacggt cgtctgcatg tgcgcttctt
     4321 accggacccc ggcgctccgt gcctaatctc cccgcgttga ccgcgaccaa gaagagcgac
     4381 tggaagccac ccaccaagcg caagctgatc gccatccgcg accgcctgcg caaggcctac
     4441 ggcaagcccg agccgcggcc gcaccgggcg cccatcgacg agctcatcct caccgtcctc
     4501 tcgcagaaca ccaacgaccg caaccgcgac gtcgcgtacg ggcggctgcg ccagcgcttc
     4561 ccgaggcgcg atggctcccc attgcccgac tgggacgcgg tggggaacgc gcccgaggac
     4621 gccgtcgagg aggcgatccg gccggggggc atctcgcgcg tgaaggcgag gcggatccag
     4681 gaaatcctcc ggtcgctggg cgacgacgac ctgacccacc tcgagaccga tcctctcgac
     4741 gaagcgcgcg actacctctg ctcgctgccg ggagtgggcc gcaagacggc cgcttgcgtg
     4801 ctgctcttct cgtacgggcg cccggacgtc ccggtcgaca cgcacgtcta ccgcgtcggt
     4861 acccggctcg gcctcttcag gcccggcgcg tcgttcgacg aggcgcacga cgagatgctg
     4921 cggctgacgg atcccgagga cgcgtacgag atccatgtcg ggctgatccg ccacgggcgc
     4981 cgcatctgca ccgcacgcaa cccgcgctgc cgcgaatgcc cgctgctgtc gctctgcccg
     5041 tacgggaaag cgcgagttcg gtgaccgagc agcaccgcgc cgaggcggcg ctggtcggga
     5101 tcgcggcggt ctgggggctc acgttcgtga tggtgcagga cgcgatcgag cagctgccct
     5161 cgctggcgtt cctcgcctac cgcttcgtgc cagccgggct gatcgtggcg ctcttcgcgc
     5221 gccgcgcgtt gcgcggccta gggcgcgcgg gctggctggc cgggcttgcg atgggagtgt
     5281 tcctcaccgc cggatacatc ttccagacca tcgggctcga gcacacgagc gcgtcgaacg
     5341 ccggcttcat caccggactg ttcgtggtgt tcacgccggt gctcgcggca gtgttcctgc
     5401 gcgagcggat cggcgcgctc gcctgggggg cagcggcgct ctcggcgctc ggcctctacc
     5461 tgctcgcggg cgcgacgcac gggttcaacg agggcgacct gctcgtgctc gggtgcgctg
     5521 tcgcgttcgc cgcgcacatc atcgtcacgg ctcgcggcgt tcgtgcccac gacgtcggcg
     5581 cgctgctcac gattcagctc acgttctgcg gcgtcgtgtg cctgatcgcc gccgcggctg
     5641 ccggcgacct cgaggttccg cgcggcggca ccgtctggtc ggcgctggtc gtcacctcgc
     5701 tcgtggcgag cgcgctcggc ttcttcgtcc agagctacgc acagcagcac gccccgccgg
     5761 cccgaacggc gctgatcatg gcgagcgagc cggccttcgc cggcttcttc ggctgggcga
     5821 tcggcggcga gacgctctcg ctcgtctcct ggttcggcgc cctgctgatc atggcggcga
     5881 tcctcgcggt cgaggtcgtg ccgcgcttca ggccgccgcg gcctttgccc gagggttgac
     5941 ccgcgcccgc tcgcgcgcaa tggtgcgcgg catgagggat ctggctcgct tcgcggccat
     6001 gggcctctac ctgttcgacc tgttcctgct ccggctcgtg ggcgccggcg cgatggcggt
     6061 ctcctcgacg ggcgcagtgc tggcgctacg aatgacgccg ggcatcctca gcatcgcggc
     6121 cgtcggcgtg gtcgcctcag ggctctggag ctatggcgcg cttcagcgcc cggtgccggc
     6181 ccgcgtgaaa ccggccacgt ggttcgcggc cggcagcatg ctcgtgtggt tcctgctgct
     6241 gctcgtttga cggcctggcc cggacgtggg tgacctttgg gtcgaatagc gaccccaaag
     6301 tcacccaccc tcccgccctt tatcgctgct ttttcgcccg accggccgct gctcaccggg
     6361 gtgggcaacc cgcgaaagat ctgccacaat cggactcaga tttctttccg caccagcgag
     6421 aaagcactac aggaggaaaa cttcaactat gggtaagacg ataggcatcg acctcggtac
     6481 gaccaacagt gcgatggccg tgctcgaggg cggcgagccg accgtcgtag aaaacgccga
     6541 aggcggccgc acgaccccat cggtcgtcgc gttcaccgcc ggcggcgagc gacttgtcgg
     6601 cactgtcgca aagcgccagg ctgtcacgaa ccccgagaac accgtcttct ccatcaagcg
     6661 cttcatgggc cgcaaggagg ccgaggtgcg cgaggaggag tcgatcgttc cgtacaaggt
     6721 cgaggcaggc ccgaatggcg acgtccgcgt ggacgcgggc ggcaagcagt actccccgcc
     6781 ggagatcagc gcgatgatcc tccagaagct gaaggccgac gccgaggcgt acctcggtga
     6841 cacggtcgac agcgcggtca ttaccgtccc ggcgtacttc aacgacgacc agcgccaggc
     6901 caccaaggac gccggcaagg tcgccggact cgacgtcaag cgcatcatca acgagccgac
     6961 cgcggccgcg cttgcctacg ggctcgacaa ggaggagtcc gaccacacca tcctcgtctt
     7021 cgacctcggc ggcggcacgt tcgacgtgtc ggtgctcgag atcggcgacg gcgtcttcga
     7081 ggtcaaggcg accgcgggtg acaaccacct cggcggcgac aacttcgaca aggcgatcgt
     7141 cgactggatg gtcaaggagt tcaaggcgtc acagggcatt gacctctcgc aggaccgcat
     7201 ggccctgcag cgcctctacg aggcggccga gaaggccaag gtcgagctgt cctccacaca
     7261 ggagacgcag atcaacctgc cgttcgtcac ggctgaccag tcggggccga agcatctcga
     7321 catgcgcctc actcgctcga agctcaacga gctcacgtct gacctgctcg accgcaccgt
     7381 cggtccgacc aagcaggcgc tgcaggacgc aggcatcacg ccgggcgaca tcgcgcacgt
     7441 ggtgctcgtc ggcggcatga cccgcatgcc ggccgtccag gagaaggtga aggagctcac
     7501 cggcaaggac ccgcaccgcg gtgtgaaccc ggacgaggtc gtggccgtgg gcgccgccat
     7561 ccaggcgggc gtgctggccg gcgacgtgaa ggacgtcctg ctcctcgacg tgaccccgct
     7621 caccctcggc atcgagacca agggcggcgt catgacgaag ctgatcgagc gcaacaccac
     7681 catcccgacg cgcaagtcgg aggtgttctc gacggccgag gacaaccagc cgagcgtcga
     7741 ggtccacgtg ctccagggtg agcgcgagat ggccatcggc aacaagtcgc tcggcaagtt
     7801 ccagctcacc ggcattccgc cggcgccccg gggcgtgccg cagatcgagg tgacgttcga
     7861 catcgacgcg aacggcatcc tcaacgtgtc cgccaaggac ctcggcacga gcaaggagca
     7921 gaagatcgaa atcaaggcgg gctccggcct gtccgaggac gagatcaagc ggatggtgac
     7981 cgacgccgag gcgcacgccg aggaggaccg ccgccagcgc gagctcgcgg aggcccgcaa
     8041 caccggcgag aacgccgcct accaggccga gcgtcagctg gccgacatgg gcgagcaggt
     8101 cgacagctcg gcgaaggagc agatcgacgc tgcgatcaag gacgtgcgcg actcgctcac
     8161 gtctgataac gcagacgaga tccggcagaa gaccgcctcg ctgcaggagg ccttccaccg
     8221 cgtctccgag cagatgtacg cgcaggcggc tcaggcgcag cagccccagg gcgacggcgc
     8281 agccaacggc gcgacgactg actcctcgga cgaggaggag gtcgtggacg ccgaggtcgt
     8341 ggatggtgat tcgaagtcgt gagcatccac gagcagcagc gacaggacga gcgggagcgg
     8401 ctcaacgacg agccgctccc gactcagcag cccgaagagg gcgagcccgc cggcgcggac
     8461 gacgagtcgg ccgagcagga gctggaggag gcgactgagc gccccgtcga cgagggcggg
     8521 gacgacctga cgcgcgcgca gcgcgagcgc gacgagtacc tgtcgctcgc gcagcgcacg
     8581 caggccgact tcgagaacta ccgcaagcgc gcggccaagg aggccgcggg tgcgggcgag
     8641 cgggcgaagg cggggctcgt gcgcgagctc ctgcccgtgc tcgacaacct cgagcgtgcg
     8701 ctcgccagcg ccaactcccc ggacgacgtg gtcgcccagg gtgtgaagct cgtgcacgcc
     8761 gatctggtcg gcgtgctgca gcgtgccggc gtagaggcgt tcgacccggc cggcgaggcg
     8821 ttcgacccga ccgtgcacga ggcgctcacg acccgtcccg cggaggacgg cgtccaccca
     8881 gggacggtgc tcgacgtggt cgagaagggc taccgcctca acgacaccgt gctgcgtccc
     8941 gcccgagtcg tggtgtccgc gtagcgatgg cagccgtccg ggatccatac aagacgctcg
     9001 gcgtcgacaa gaaggcctcg caggacgaga tcaagaaggc ctaccgcaag ctcgcgcggc
     9061 agtaccaccc ggaccagaac ccggaagacc cgcacgcgga ggagcgcttc aaggaggtcc
     9121 aggaggccta ctcggtgctg tccgatcccg agaagcgcaa gcagtacgac cagggcggcg
     9181 gcatcttcgg cggcttcggc gccggcgggt tcgacccggg ctcgttccgc accggcggcg
     9241 gcggtggtgg tttcggcggc atcggcgaca tcctctccga cctcttcggc ggcggtggtg
     9301 ccggcggccc gcgggcccag cagccggagc gcggccgcga cctcgagacc gaggtgcaca
     9361 tttcgttcga ccaggcgatg aagggcgctc aggtgccgat cgtcgccact atcccgtcgc
     9421 cgtgtccgac ctgtcacggc acgggcgcac ggccgggcac gagcccaacc gtctgcccga
     9481 ggtgtcaggg tcgcggcatc gagtcccagg gccagggtct gttctcgatc tcccagccgt
     9541 gctcgctctg cggtggcacc ggcacgcaga tcagcgatcc ctgccccacc tgccacggcc
     9601 gcggccagac gccgcagacc aagcgctacc gcgcgaacat ccccgcgggc gtgcgcgacg
     9661 gcagccgcgt tcggctcgcc ggcaagggcg aggccggccg gcgcggaggg ccgcccggcg
     9721 acctctacgt ggtcacgcgg gtggaggagt cctcgatctt caagcgcaag ggcgacaacc
     9781 tcgaggtgga cgtgccgatc acgatcgtcg aggcgatccg cggtgcgacc gtggaggtgc
     9841 cgacactgaa cggctcgaag cgaatccgtg tgccggcggg cacgcagcac ggcaccgtgc
     9901 agcggctgcg cggcgagggc cctgcgcgcc tcaacggcaa gggccgcggc gacattcact
     9961 accgccttgt gatcgacgtg ccccgctcgc tcagcaagca gcaggaggac gcggtcgacg
    10021 ggctcgccga ggtcctcaac ggcaatccgc gtgagcggct cttcgcaggg agggatcgct
    10081 gatggcgcgc cgcaagcagc tcgatcctcg ccagggcgtg tacatgatct ccgtggccgc
    10141 gcagctggcg ggcatgcacc cgcagacgct ccgcatctac gagtcgcgcg ggctgatcac
    10201 gcccaagcgc tcgcagggca acacccgcct gtattcgcag acagacgtcg agcgcctacg
    10261 ccgcatccag gagctcacct cggagatggg catgaacctc gccggcgtcg agcgcgtgtt
    10321 cgagctcgag gagcagctcg agcgcatgca ggacaagatg ctcaagctcg agcgcgaggc
    10381 cgagcaaatg caagaggagc tcaagctcga gatcgagcgc gtgcagcgct ccttcaagtt
    10441 cgagttggtg ccgtacgagg agccgggcca cgccctcatc ccggcgaagg aagccgccaa
    10501 gggcgtgcgc ataccgatca cgcgccgcga cggcgcccgc tgacggtgct cggcgacgcg
    10561 cagctcctga cccgtgtcta tgacgcgttc aacacgggcg gggtccgcgc cgtcgccgac
    10621 tacatccacc ccgacgcgga gttcatcgac ccggtggagt tcccgggcgc cgaccactac
    10681 atgggccgcg aggcggtgat ctcctatctg gagaacttcg ttgaggcgtg gggcgtggtg
    10741 aacatcgtga tcgaggagtt ccacggcgcc ggcgggcgcg tgggggcgca gctgtgcatc
    10801 gagctgcgcg gcgccgcgag cggctacgag accgagatgc gcgtgatgca cgtgatcgac
    10861 atcgaggacg gccaagttcg gcgtgtgcag ggctttttcg accgcgagga gggactcgcc
    10921 gcgctggtgg gcaagcctgc atagatctac tacactcgca cttagatttt atgaacgtag
    10981 accgcttcac aatcaaatca caggaggccc tccaggccgc tcagcgcctt gcgggcgcgc
    11041 ggcgcaatcc ccaggtcgcg ccgcagcacc tgttcgcggc gctgctcgag caggagggca
    11101 gcatcgtggt cccggttctc cgccgggcgg gcgcggacgt cgaaggcgtc cggcgccgca
    11161 acaacgagaa gctcgacggc ttccccacgg tctccgggga cgccacccag gcgccgtcgc
    11221 tcagccaagc cacgatcgac acgcttcaga aggcggacga ggaggcccgc ggcttcggcg
    11281 acgagtacgt ctccaccgag cacctgctcc tcgcgcttgc gtccgacccg aacatcgacg
    11341 ccggcgcgac gcgcgaccag ctcgccgacg ccatccaggc ggttcgcggg ccacaccgcg
    11401 tcaccgacca gaaccccgag gacaagtacc aggccctcga gaagttcggc cgtgacctca
    11461 ccgccgcggc ggaggagggc aagctcgacc cggtgatcgg ccgcgacgac gagatccgtc
    11521 gcgtgatcca ggtgctctcg cgccgcacca agaacaaccc ggtgctcatt ggcgagccgg
    11581 gcgtcggcaa gaccgccatc gcggagggtc tcgcccagcg catcgtgtcg ggcgacgtgc
    11641 ccgagtcgct gcgcgaccgc cgcgtgatcg cgctcgacat cggcgcgctg atcgccggct
    11701 cgaagtaccg cggcgagttc gaggaccggc tcaaggccgt cctcaaggag gtccaggagg
    11761 ccgagggccg cgtgatcctc ttcatggacg agctgcacac gatcgtcggc gcgggcgctg
    11821 ccgagggcgc ggtcgatgcc gcgaacctgc tcaagccgat gctcgcccgc ggcgagctgc
    11881 gcgcggtggg cgcgaccacg ctcgacgagt accgcaagca catcgagaag gacgccgcac
    11941 tcgagcgccg cttccagccg gtgatgatcg acgagccgag cgtcgaggac gcgatcgcga
    12001 tccttcgcgg gctcaaggag cgctacgagg tgcaccacaa cgtgcgcatc caggactccg
    12061 cgatcgtcgc ggctgcgctg ctctcgcagc gctacatcgc ggaccgcttc ctgcccgaca
    12121 aggcgatcga cctcatcgac gaggccgcct cgcgcgtgcg catcgagatc gactcggtcc
    12181 cgaccgagat cgacgaggtc gagcgccgcg tcatgcagct cgagatcgag cagcaggctc
    12241 tgtcgaagga ggaggacgag gcgtcggtcg cccgccgcga ggccatcgag cgagagctcg
    12301 ccgacctgcg cgagcagacg aacgcgatga aggccgagtg gcagcgcgag aagggcgctg
    12361 tcgacgcgat cgccgacctc aagaagcgac tcgacgaggc ccaccgtgag gtggagcgcg
    12421 ctgagcgcga cgccgacctc gagcaggcag cgaagcttcg ctacggcgag atccccgagc
    12481 tcgagaaggc gctcgaggaa gccgagcaca cggtcgaggg cgagcgcaag ttcttcaagc
    12541 aggaagtcga cgacgaggac gtcgccgagg tcgtcgcgaa gtggaccggc atccccgtct
    12601 ccaagttgat ggagggcgag gtcgagaagc tggtccacat ggaggatcgc ctccacgagc
    12661 gcgtggtcgg gcaggacgac gcggtcgagg ccgtgtcgaa cgcgctgcgg cgctcacgtg
    12721 cgggccttca ggacccgaac cggccgatcg gcaccttcct gttcctcggc ccgaccggcg
    12781 tcggcaagac cgagctggcg cgggcactcg cagagttcat gttcgactcg gagcaggcga
    12841 tggtgcgcat cgacatgtcg gagtacatgg agaagcacgc ggtgtcgcgg ctcgtgggcg
    12901 cgcctccggg ctacgtcggc tacgacgagg gcggtcagct caccgaggcg gtgcgccgcc
    12961 ggccctactc ggtcgtgcta ctcgacgaga tcgagaaggc acaccccgac gtgttcaacg
    13021 tgctgctgca ggtgatggac gacggtcggc tcacggatgg ccagggtcgc acagtggact
    13081 tcaagaacac cgtgctgatc atgacctcga acgtgcccgg cggaatggcg ggcgtggagg
    13141 cgaccttcaa gcccgagttc atcaaccggc tcgacgagat cgtccagttc caccagctca
    13201 cccgcgagga gatcggctcg atcgtggaga tgcaggtcgc gaagctctcc gcccggctgg
    13261 ccgagaaggg catcgagatc gagctcaccg acgacgcgac catgctgatc ggcaacctgg
    13321 gctatgaccc gacctacggc gcccgcccgc tgaagcgggt gatccaaaag cagctcgtgg
    13381 acaagctcgc gctggcactg ctcgagggcg agttcgccgc aggcgacagg gttctcgtcg
    13441 acgccgagga cggcgagctc aagttcagca aggccggcgc ggtagcgccc gccgccgtcg
    13501 cgtaagagcg accgcggcgg gctcgccccg cgctacttct tcttcgcgag cttcagcgtg
    13561 aggctgcgcg tggactgcag cgacccggtg gtcgccttcg ccttcacgcg cagcttcttc
    13621 ttgcgcgcga ggattttccg cgcctgcttg gtgagcttca ccttcacctt ggcggacttg
    13681 cccttggcga tcttgaactt ggccgtgccg gccttgatcg tcttggcggc gttcgacgag
    13741 gtggtgagcg tcagcgtgcc cgcgcagtcg acgctgcttg cggccgggca gccgagcggc
    13801 acgtacgcgt agttgccgga gaccgtcccg ttgccggcag tgaaagcaac gggcatcggc
    13861 gtcgccgtgc cgctgcttgt cgccttcttc acgacgttca ccgtgaaggc ggcgctcgac
    13921 gcgttgccga ctgtgtcctt ggccgtgcat gtcaccttcg tcgcaccaac tgggaacgtg
    13981 cttcccgagc ccggcgtgca cgccacggag cccggctcga cgcccgaggg gtcggtggcg
    14041 gccgggaggt cgtagaagat cacggcgttg tcgccgttcg tctcgacggt cttgtcgctc
    14101 acgggaagag ccgggccctg cccgggagtc acggttacgg tgaacgtccc gttcggcgat
    14161 aggaacttga atcccccgat gcaggtcggg tcgacgatga ccgaacacgg ttgcatcacg
    14221 agccctatgc agctaaccgg agtcgtcccg accacgaagg tggcgcccgg taccggagtg
    14281 catgtggctg gtcctatgag aggcccctcg gtcgaagtcg tcgggagtgt gtagctcacc
    14341 gggacggcca gcccgcttgg cccggtttcc ttggtgatgt cgccgtcggg aaagatgatg
    14401 ctcgcgttcg cggaagcggc gaacaccagc agcgctgcga tcgcgcccag cgtggcgcgc
    14461 gagatcttcc tcatcgagtc ctccagggtt gggcgagtct ggccggttcc tttgtaaccg
    14521 gtcaccgaat ggtgcaggga ccccccggac ggatgttcta tgcgcccttt cggggcaccg
    14581 ccgaagacct caggctgcct cggcttgctt cagttcgacc gttccctgat tcgtgcggat
    14641 ggcgaaagtc ggctgcggtc cagtagcggg gcccttgacg accgatccat cgaggcggaa
    14701 ggtgctcccg tggcaggggc acgtcacggt gttgtcgtcg ttcagccggc cctcatggag
    14761 cgagcagcca cgatgtgagc accgatcggc gagcgcatgg atgcgcccgt ggttgcgcac
    14821 gagcaggacc ccgaggccgt tggcccgcgc gccaatcagc ttcccttcct cgagcgctcc
    14881 ctcctcgatc accggtgtcc agtctgatgg tgcgtcctcg aacgccgtct cattgacgcc
    14941 gacgccgagc ccgaacgaga ggtggccgcc gagcaaggcc gagaacgttg cgaagccgta
    15001 accgagcgcc gacagcgcga tgccgcggcc tcgctcaccc cgtctgcgcg ccgcccaaga
    15061 gaacgtatgc aacacgattg cggtcacgtt gccggtcgcg tggacgatgg cgactcggcg
    15121 gggcccgccg cgcagctccg cagcgtccga gaggcccgcc gtcgcggtcg ggacggctgc
    15181 caggactccc acgcccacga gcccgtcagc tgatgactcg gcaccgtcac cgccgaacag
    15241 atcgaggagc agggcgctcg tccacgcccc aacgaccacg tcggtgagcg gcggatgaag
    15301 ggggtgcccc agccaggtcc cgctgagaag atccttcagc ttggagtcct cgggcacggc
    15361 agcccgcact cctttttgca atgggtcagc cagggcgtcg aggctcgcga gccgctcgat
    15421 tcgctccccg atgttctgtc gcacaaccgg ttccaagccg ccttgtgggc ccgcctcacc
    15481 agccggctgc tggcggtccg ccgcaacgaa gcgtgtcatc gggtctcctc ctccacctag
    15541 atctgtcaag cgattctccg agcctcttga tctacccggc cgcgtcgtcg ctacgcgcgc
    15601 gcctcggcga gatgacttgt gcccatggga ggatcggtgg ccgtggtgga tccggacgag
    15661 cagatcgcgg cccgcggcga agacggacgc cggcgctgcc gatgggcact cggcacgccc
    15721 gactacctcg cctaccacga cgaggagtgg ggccgaccgg tgacgggcga ggacgagcta
    15781 ttcgagcggc tctcgctcga ggcgttccag agcggcctgt cgtggctcac catcctgcgc
    15841 aagcgcggca acttccggcg cgcgttcgcg ggcttctcga tcgagtgcat cgcggcgttc
    15901 gggcccgccg atttcgaccg cctcatgaac gacgcgggca tcgtccgcaa caggctgaag
    15961 gtcgaggcgg tgatggcgaa cgcgcacgcg gcggcggaga tgcacgctcg cggcgagttg
    16021 ctgtcagagc tgttctggtc gcatcgcccg cgcggacgcc agagggctcc gcgcacctgg
    16081 ggcgacgttc cggcgaccac gcccgagtcg gaggcgctcg ccaaggagct gaagcgccgc
    16141 ggcttccgct tcgtgggccc gacgaccgtg tacgcctcca tgcaggcctg cggcgtggtg
    16201 aacgatcacg ccgcaggctg ctgggtacgt ccggaggtag agcgcgagcg gcgtcagtcc
    16261 ttggagcaga cgtagtagac gttgaagaaa tcgccgtcca cgcgctgcac cgagaccttg
    16321 gcgaagccgg cgtccgcgag catctgctgc gccagctcct cgccccacat ggtgcccaga
    16381 ccggctccgt gctgggccag ggacaccgtc atgcagtgca tgagcgacac ggtgtacagc
    16441 gccgggccca gagggtggtc ggcgttgcgg tccacgtcgc tcgagccggc gatgtcgacc
    16501 atcaggtagg tgccgccggg cttcagcgag cgtgagatcg cagcgagtgc gcccgcgggg
    16561 tcggcgaggt cgtggatcac gtcaaatgcg gtaatcaggt cgtactcgcc ctcgatgtcc
    16621 gtcacactcg cctgctcgaa acgggcattc gtgagcccga gggcgttcgc ctcctcgcgg
    16681 cctgcggcga tcccctcttc ggagatgtcg cggccggtga agcggctgtt cgggaatgcc
    16741 ctggccagga cgttcacggc gtggccctgt ccgcagccga catcgatcgc gtcgatgccc
    16801 tgctcgaggc gctccgtcag cccgggcacc agcggaacca caccgtgcac gagcgccgcg
    16861 tcgtacacct gagctgtctc ctcggcctga agggcctgga agcgcggata cgcggaataa
    16921 ggcacgccgc cgccgttgcg gaagcactgg atcaccggct cctccacttc ggcgagcagg
    16981 ccgacgtact gcgcgagcgt ggcgacgttg tcaggcccgg ccgctcgagt gatcgccgcc
    17041 gcgtgctcgg gagggagcgc gtacgtgttg gactcgctgt cgtgaacgag cacaccggcc
    17101 acggtcaggc cgtttgccca ctcgcggatg tagcgctcgt cgagcccggt tgcctcggcg
    17161 atctcggcgc tggtagacgg gggcagctct gcgaggctgt cgaacaggcc gagccggtgg
    17221 ccgatgctca cgaggagcga cgtcatgccg ccgttcagga tcccgatcat ctggcccgcg
    17281 aactcctcgg cgcgtgtggt atcgatctgc tcggttgcca tgcggctctc ctttgattgg
    17341 ggtgccgcaa gcctctcccg cgcgcggggt cgcgcgcatc ggggcccaca cgtaaccgca
    17401 cacgtagagc tcagcggcgg aagtcccact ccggcatcgg gtgggacgac gagatctggg
    17461 cgcccgcgct ttcgtaggtg gcctggccgg cttcgttgtc gtggtcggtg agcgtccaca
    17521 tgccgtagca cccgcgctcg cgcgcgagca ccgcgagcgc ctccacgagc ctgcgccgat
    17581 gccgcgccgc cgcgacggct cctgcacgct cacctcgtag acgaacatct ccgtgccctt
    17641 gtccggatgc gtcagctcca cgccggacac gaatccgact ggctgatcgg agtcgtcgta
    17701 ggcgatcagc aggtggtggt tggggtcctc gagaaagcgc cgcgtcgcgt cttccagtgg
    17761 agcgctgtcg aagagcgagc cggcagcggt cagcgcgtcc tcgtcgccgg ggccgagacg
    17821 gcggatctcg atgtccatca cgagatgcgc gccgcgcgcg acagcgcgta ggcggtcgcg
    17881 ctcgcgcgcg cggagggtcc cgagactccg agcttgagat agatgttcga cacgtgccgc
    17941 tcgacggtac ggacgctgag cacgagcgcg gcggcgatct cctcgttcga gaggccgtcg
    18001 gcgagcagcc gcagcacctc gacctcgcgc cggctcagcc ccgcggggtc ggacggcgac
    18061 tcggcggcgg ccctcgcgag cgccgcggcg cgctcggcgt cgagccgcgc tcccacgtgc
    18121 aagagcgtct cgcgcgcgcg gcgcgcctcg gcggcagctt ccgcgccggt tccgagcgcg
    18181 gccgccagct cgagccgcgc ccgccccgcc tcgtagggcg agccgcttcg ctcgtagagg
    18241 tcgactgcgt cctcgaggaa cgcacgtccg cgctcgtcct cgcccgacgc taccgccacg
    18301 aggccctccg acagcgcggc cgccgcagcg agcgggtcgg tgccgatagc gtcggcgatc
    18361 gcccgtagct cgcgcgccgc ctcacccgcc ccagtcattt cgccgagcgc caggcgcgag
    18421 cgcacgagca cctcgaggcc cggcacacgc tgcgtgcgat cgagcggcga gacccggcgc
    18481 aggaagcgcg ccgcacgcgt cgccgcggcg gccggatcgc cccggtcgag cgcgagccag
    18541 gcgagcccga gcgcgcccat cgcgtgcgag tccgcctgtg cgagcagccg ctccgcctcg
    18601 tcgaggcgcc cctggcggcg acgcagctcg ccgattcgcg cgaccgcgtc gtgctcgtgc
    18661 gcgccgggcg tggcacccac cgcgcgcacc acctggtcaa gttcgctctc ggcctcggcc
    18721 cagtcgccgc gccaggtgag cacgtcggcg tagtgagtgc gacagatggc gaacagcagc
    18781 tggtccgacc accgctcgga catctcggtc acgcgcgcgc accactgctc ggcgcgctcg
    18841 aagtcgcgca cgcgcttgca cgcgtcgatc aggtagcagc acacggttgc gatggcgtcc
    18901 acgctcctga gctcccccgc cagcgcggcg gcggtcgcct cgtcgagccg gcgcatgccc
    18961 tgcgcgacgt cgccgtcgct cacgaggatg aggccctcga gcgcgttgcc cagcatctga
    19021 atgtcgaggt cgccgttctc gcgcccgatc tggtgggcgc ttcgtgcgag ttcgagcgcg
    19081 cgcgcattgt cgccgccgcg cagcagcagg atgtgcgctt cgaagccctc cacccacgca
    19141 agctcgcgca cgtcgcccgc gccgtcgagc agtgcgcggg cgcgctcgag ccaaccgagt
    19201 gccaccgccg tgccctggcc catgtcgtat gcgcagttgg cgatccacag cgccatccag
    19261 gcagcgccac ggctgtcgtc gttctcgcgc gcgaggctgt agccggcctg gtacgcctcg
    19321 gcggccgcgc ggctgtcgta gaggcgccgg cacgcctcgc cgacgcggtc gaacgcggcg
    19381 acggacggct cctccgcgac cgctgcctcg aactcgcgcc gcgcctcgct ccagtcgccc
    19441 cggcgcatgg cgtcttcacc ccgcgatgtg tgttcggagg gcggtcggtc gcttcctggg
    19501 cctcgggcag tggccaatgc ggctctccta cggagaacct accttccgct acgcgggctt
    19561 gcgagcggcg ccggcgacgc cctcgttcac gaggcggggc accttgggtg ccttctcgcg
    19621 cgcgctgaac tcgagttcga gcggcgagcg ctggatggtt gcgagcgtgt cgcggttcgg
    19681 gtggcagcca cacgcgaacc agccccacgg gcgctcgacg cgatcctgcc agcgcgcgat
    19741 cttcgcgtcc tcggagcgca cgtgctccat gaacagcatg cggccgccgg gcttcagtac
    19801 gcggtggatc tcggtgagcg cgtcgctctg gtcggggacc gtgcagagca cgagcgtcac
    19861 gacgacggtg tcgaacgtgt tgtctgggaa cgggaggcgt tctgcaggcg cctgcacgac
    19921 ctgagcggcg cgcccggacg cggcgacctt gtgctcgagc ttggacgcca tgtgggggct
    19981 cggctccgcc agcacgagct cctccacgtc gccgtagtgg tcgaggttga ggcccgtgcc
    20041 ggcgcccacc tcgaggaccc ggccggacgc ctgcgagacc agcccgcggc gcatctcgcg
    20101 cacgccggcc tcctcggcgc ctttctgcag gcggtcatag accgctgcga atacccgtcc
    20161 ccatgtgttc tcggcaagct tgcttggcat ggggccgacc ctaccgcgcc gacgcgcctc
    20221 ggcgcgtcct tgcatcggat ccggaaagat gcgacaaacc caggcacttg gatccggagc
    20281 gaaaggacgg ccatgcccac gttcgtgctg ctcagcacgc tcactccaga aggcgttcag
    20341 accgtgaaga acaacccgac gcggatcaag gaggtcaaca aggaggttga gcagctgggc
    20401 gcaaaggtag tggcgcagtg ggccacgctc ggccgcttcg acttcgtgaa catcgtggag
    20461 gcgcccgacg agcagacgat cgcgcgcgtc tcgctcgagc ttgggtcgcg cggcacgtcc
    20521 aagtatgagt cgctcgtggc aatccccgtc gacgacttca tcgcggcgct ctgatttgaa
    20581 ggtcctcgtc gtaggcggcg gcgggcgcga acacgcgctc gtccgtgcac tcatgcgcag
    20641 ccctcagcag ccggagatcc tcgccgctcc gggcaacgcc ggaatcgcgc gggacggaat
    20701 tcgtttgttg ccggtgggcg ccgaggacgt gagcgcgctc gtggcggcgg ctcgtgagga
    20761 gcgtgtggac ctcgtcgtgg tcgggccgga ggcgccgctc gtggcgggcc tcgtcgacgt
    20821 gctcgccaac tcgggtgtgc gcgcgttcgg cccgagcgcg gcggccgcgc ggatcgaggg
    20881 gtcgaagacc tactcgaagg agctgatgcg ccaggtcggc gtccccacgg ccgagtacgc
    20941 cgtgctgaag agccgcgagg aggcggagga gcacatcgcc ggcgcggcct acccgctcgt
    21001 gctgaaggcg gacgtgctgg cggccggcaa gggcgtgatc atctgcaccg acgagcagga
    21061 ggcgcgcgcc gcgctcgacg agttcttcct gcaggagcgc ttcggccgca cgacggtggt
    21121 cgccgaggag ttcctcgagg gcgaggagct ctcgctgctc gcgctctgcg acggcattcg
    21181 cgcggttccg atggcaccgg cgcgcgactt caagcgcatc ttcgagggtg atcagggccc
    21241 gaacacgggc ggcatgggca gcttctcgcc ggtgcccggg atcgactccg accacgcgcg
    21301 cgcgctcgcg cgcgcggtgc accagccgat cgtggatgag ctgcgcagcc gcggcacgcc
    21361 ctaccacggc gtgctctacg cgggcctgat gatgaccgcg gaggggccga aggtgctcga
    21421 gtacaacgcg cgcttcggcg atccagagac gcaggcgatc ctgccgcggc tacagtcgga
    21481 cgtgctcgaa ctgttcgacc gcgcctcgcg ccccggtggg ctcgagggtg tcgagctgga
    21541 gtggtcgccc tcgtgggcgg tcacggtcgt gctcgcctcg cgcgggtatc ccgagtcctc
    21601 ttcgaagggc gacgtgatca cgggcctcga gcaggtcgcc tcgctcgacg ccgaggtcct
    21661 gcacgcaggc accgccgagg aaaacggccg catcgtgacc gccggcgggc gcgtgctgaa
    21721 cgtgacgggg atggggccga ccgtggctga ggcgcgtgcc cgcgcctacg cggcggccga
    21781 caagatcgaa ttcgacggca agcagctccg acgtgacatc gcggcgcggg ttgccgagga
    21841 ggtgaatgcg tgacggagga gacctcgggc gcgcccgcgc ccgcaaccga gaccgcggtg
    21901 gagagcgctt tcgacgagct cgacgttgat gcgccgctcg tcgggatcgt gatgggatcc
    21961 aagagcgaca tgccggtgat ggagaaggcc ggcaacgagc tcgaggagcg cggcatccgg
    22021 tacgagatcc gcgtgatgag cgcgcaccgc gaccccgagg tggtcgccga ctacgccgtc
    22081 aacgcacgca tgcgcgggct caaggtgatc atcgcgggcg ccggcctcgc cgcagcgctc
    22141 cccggcgtgg tcgcagcgca caccgacctg ccggtgatcg gcgtgccgct cacctcggcg
    22201 acatccgtgg gcggcggcct cgacgcgctg ctcgcgatcg cgcagatgcc gcccggcgtc
    22261 ccggtggcgt gcatgggcgt gaacggagcc aagaacgccg cggttctggc tgccaagatc
    22321 ctcgacgcgt gatcgagcga tacacacggc cggagatcgg cgcggtctgg tccgagcatc
    22381 ggaagctcga gacgtggctc gctgtcgagc ttgcggccgt cgaggtgctc ggcgagcagg
    22441 gtgtgatccc gcccggggac gtggaccaga tccgcgaccg ggcctcgttc acggtcgagg
    22501 ccgtccagga gcgagagaag atcaccgacc acgacgtcgc cgcgttcgtc gacgtggtcg
    22561 ccgagtcggt cggcggcgac gcgggccgct gggtgcatca cggcctcacc tcctccgacg
    22621 tgctcgacac cgcgcttggt ctccagatct cgcgcgccgg gatgatctgc atcggtggcg
    22681 cctgcgactt ccgcgacgcg ctgatcgagc gcgcgcgccg gcacgtccac acgctctgcg
    22741 tggggcgcac gcacggcgtg catgccgagc cgaccacgtt cggcgtgaag ctcgcgggct
    22801 ttgccttcga ggcgcaccgc aacgttctgc ggctggagcg ggcggttgag caggcatcgg
    22861 tcggagcgct gtccggagcg gtcggcacct acgcatcgaa cgggcccgaa ttcgaggtgc
    22921 gcgtgctcga gcggctcggc cttgcgcgcg aggacgtctc gacgcaggtc gtcgcgcgcg
    22981 accgccacgc gcagctgctc tccgcgatcg cgatcgccgg cgcggggctc gagcgcttcg
    23041 ccaccgagat ccggcacctc cagcgcacgg aggtgcgcga ggtggaggag ccgttccgcg
    23101 caggtgccca aaaggggtca tcggcgatgc cgcacaagcg caatccgatc gtctccgagc
    23161 gcatcaccgg catcgcacgg ctgctgcgcg gctacgcgca agccgccacc gaggatgtcg
    23221 cgctatggca cgagcgcgac atttctcact cgtcggtcga gcgcgtcaat ctgccggacg
    23281 ccacgatcct gctcgactac gcgcagcacc tggccacgcg cgtcgtgaac ggcatggtgg
    23341 tacacgagga ccgcatgcgc gagaacctga acctcacgca cggggcgctg ttctcccagc
    23401 gggcgctgct cgcgctggta gaggcgggtc gtacgcgtga cgaggcctac cggatcgtcc
    23461 agaaggacgc gcagcgggcc tgggaggagg ggatcgagtt ccgcgagctg ctggcgcagg
    23521 acgcaccgga tctcgacctc gacgcgatct tcgatccaag cgccttcgtg cgctacgcgg
    23581 acgagatcgt ggggcggctc gatcgcatcg ctcctcggtc gcttgacacc gccgaggcac
    23641 gggcagacga tcgggtcggc ccatcagttc cctgagtaga agggagtgcg tcgggggttg
    23701 accggtgcct cctggcggcg aggccgcaat ctccgaggat ctgaccgcct ctccgtggca
    23761 aagtcgactc cggcggttgt tagcctcaga gccatgacgc aagccgcccc gctcgcgggc
    23821 tacgacctcg ttgccaaggg caaggtccgc gagatctacg acgcaggcga cgaccgcctg
    23881 ctgctggtcg catccgaccg catttccacc tacgacgtcg tccacccgac gccgatccct
    23941 ggcaagggcc aggtgctcac cggcctgtcc gtgttctggt tcgagcggac ggccgacatc
    24001 gtcccgaacc acctgatctc ctacgaggac gtgcccgacg aggtgcgcgg ccgcgccctg
    24061 ctcgtgaaga agctcgacat ggcgcccgtc gagtgcgtcg cgcgcgggta catcaccggc
    24121 tcgggctgga aggactacct tgcgaccggc gcggtgtgcg gtatcgagct ccccgccggg
    24181 ctgcaggagt cggagcggct gcccgagccc atcttcacgc cggcgaccaa ggccgacgtc
    24241 ggcgaccacg acgagaacgt ggacttcgac cgcgcggcgg agatcctcgg cgatcgtccg
    24301 ttgctcgagg agctgcgccg cctcacgctc gaggtctact cgtt
//
LOCUS       DASVXS010000243         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_493419, whole genome shotgun sequence.
ACCESSION   DASVXS010000243 DASVXS010000000
VERSION     DASVXS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493419"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /locus_tag="VF752_13855"
     CDS             <1..1954
                     /locus_tag="VF752_13855"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8856677.1"
                     /translation="TGSDPTGLNGSIIRVDPATGAGAPGNPLSASSSANERRIVAYGL
                     RNPFRFAIRPGTSELWIGDVGWRTWEEIDRLPNPADSTADNFGWPCYEGAGIQPGYQA
                     GGFGICSSLYAAPGSVTAPYLAYDHADPVVPGEGCSTEDGSSITGITFTGSNSYPALY
                     RNALFFADAARNCIWYMSAGSDGLPDRSRVGDFVQLAGHPVDLETGPDGNVWYVDLVD
                     GELHRIEYTAGNTPPTAHATASPTAGNVPLTVHFDATGSTDPDPGEQAALSYSWDLDG
                     NGSFGDSSSASPSFTYTAPGSHTARVRVTDPHGAFDTESVAVVAGASPPNVAIDSPAP
                     SFTWAVGDAIRFSGSASDPQDGSLPASDFTWSVVMHHCTAVGCHEHHLQDFAGVKSGS
                     FTAPDHEYPAYLELEVSVKDSYGLTDEQSIRLDPKTVSVTLASDPPGAPIAFNGGVDT
                     APFTRTVIQGSINSVAAPDSTTASGVSLPFVSWSDSGARAHDIEAASSGTYTARYDTS
                     RLQASLATPGGSPFGGTSASPRLALRRDLRALALALARQGESKLAKRGSVSVPLAAGH
                     PPGRLRVSAYALRASSARSLRLLGGAAVISPKSKRFTLRLTRQARGWLASHRQAKLSL
                     HGWFRGAEGRVYTSRQAVKLRRAGRR"
BASE COUNT          311 a          788 c          716 g          318 t
ORIGIN      
        1 gaccgggtcc gatccaaccg ggctgaacgg ctcgatcatc cgcgtcgatc ccgccaccgg
       61 cgcgggcgcg ccgggcaacc cgctgtcggc gagcagcagc gcgaacgagc ggcggatcgt
      121 cgcctacggc ctgcgcaacc cgttccgctt cgcgatccgg ccgggcacga gcgagttgtg
      181 gatcggtgac gtggggtgga ggacctggga ggagatcgat cgcctgccga accccgcgga
      241 ctcgaccgcg gacaacttcg gctggccctg ctacgagggc gctgggatcc agccgggcta
      301 ccaggctgga ggctttggca tctgcagctc gctctacgcc gccccgggct cggtcacggc
      361 gccctacctc gcctacgacc atgccgatcc cgtcgtgccg ggagagggct gctctaccga
      421 ggacggctcg tccatcacgg gcatcacctt caccgggtcg aactcctacc cggcgctgta
      481 tcggaacgcg ctcttcttcg ctgacgcggc acgcaactgc atctggtaca tgtcggcggg
      541 ctccgacggg ctcccggatc gctcgagggt cggcgatttc gtgcagctgg cgggccaccc
      601 cgtggacctc gagacgggcc ccgacggcaa cgtctggtac gtcgatctcg tcgacggcga
      661 gcttcaccgc atcgagtaca cggcgggtaa cacgcctccg accgcgcacg caaccgcctc
      721 acccacggcc gggaacgtgc cgctgacggt gcacttcgat gcaaccggct cgaccgatcc
      781 cgaccccggc gagcaggccg ccctgagcta ctcgtgggat ctcgacggca atggcagctt
      841 cggcgattcg agctcggcca gcccgtcgtt cacctacacc gcccctggct cgcacaccgc
      901 tcgcgtgcgc gtgaccgacc ctcacggcgc cttcgacacg gagtccgtcg cggtggtcgc
      961 aggcgcgagc ccgccgaacg tggcgatcga ctcgccggcc ccgtcgttca cctgggcggt
     1021 gggcgacgcc atccgcttct caggctcggc gagcgatccc caggacggct cgcttcccgc
     1081 gtcggacttc acctggtcgg tcgtgatgca tcactgcacc gcggtcggtt gccacgagca
     1141 ccaccttcag gacttcgccg gcgtgaagag cggatcgttc accgcgcctg accacgagta
     1201 cccggcttat ctcgaactcg aggtatccgt gaaggactcg tacggcctca ctgacgaaca
     1261 gagcatccga ctcgatccga agacggtgag cgtcacgctc gcctcggacc cgcccggtgc
     1321 gccgatcgcg ttcaacggcg gcgtcgacac cgcgccgttc acgcgaacgg tcatccaggg
     1381 gtcgatcaac tcggtcgccg cgcccgacag cacgaccgcg agcggtgtga gcctgccgtt
     1441 cgtctcatgg tccgattcgg gggcgcgcgc ccacgacatc gaggcagcgt cgtccggcac
     1501 ctacaccgcg cggtacgaca cgtcgcgtct ccaggcgtcg ctcgccacgc ctggcggctc
     1561 gccgtttggc ggcacctcgg cgagcccgcg cctcgcgctg cggcgtgacc tgcgcgcact
     1621 cgcgctcgcg ctggctcggc agggcgagtc gaagcttgcg aagcgcgggt ccgtcagcgt
     1681 gccgctggcc gcgggccacc cgccggggcg gctgcgcgtg agcgcctacg cccttcgcgc
     1741 ctcgagcgcc cgctcgctcc ggctgcttgg cggagcggcc gtcatatcgc cgaaatcgaa
     1801 gcgcttcacg ctccggctca cgagacaggc gcgcggctgg ctcgcttcgc accgccaggc
     1861 gaaactctcg ctgcacggct ggttccgcgg cgcggaaggc cgcgtataca ccagccgcca
     1921 ggccgtgaag ctgcgacgcg ccgggcgccg ctgaaggcac ttgaacgaaa aagcctccgc
     1981 cccgcggacg ctgcggggcg gaggcttagc ttcgtggtgc tacctacgct gccaccggga
     2041 acggaggcac cgggtggttc gcgcggaaca gctcgtccgg gtcgtaggcc gcccggatct
     2101 tccgcaggcg caggtacgcg gcgggcgacc aga
//
LOCUS       DASVXS010000244         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_423261, whole genome shotgun sequence.
ACCESSION   DASVXS010000244 DASVXS010000000
VERSION     DASVXS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423261"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VF752_13860"
     CDS             <1..709
                     /locus_tag="VF752_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8856678.1"
                     /translation="GAYAEYAVANPIGTFPIPDGVGDGVALALLIQGLTAWHLYRTSA
                     KLQPGETVVVHAAAGGVGNLAVQLGKAMGAGRVIATASSEDKRQAALEIGADAAVDVT
                     RADLGDALREANGGNKVDVVLEMAGGRVFDESLSVLAPFGRLVTYGIASREPNQVSSG
                     TLMRKSQAVVGFWLFHLFQRPEMLREPLADLFERAARGELKPLVGTTYPLSDVARAHE
                     DMQARRTSGKLLIDPSA"
     gene            735..2177
                     /locus_tag="VF752_13865"
     CDS             735..2177
                     /locus_tag="VF752_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8856679.1"
                     /translation="MTTFADLGLSEVTLEALAHLGYERPTPIQEQAIPELLAGHDVIG
                     QAQTGTGKTAAFGLPLIEYVDPSLEEIQALVLTPTRELCIQVTQALRAYGERKGVNVV
                     ATFGGAPIRGQVAQIKGAHVAVGTVGRVMDLMNRHELVLTDTRYVVLDEADEMLDLGF
                     FEDVETILSRAPSGRQTALFSATIPPPIKELAEKRMFDPITIKVRAAQLTIDTVEQFY
                     LEVPDREKADTLAQVLKAERPDQAIVFVRTKIGADRLARSLNDRGVRVKALHGDMSQG
                     SRDGVMLAFKGGRDRLLVATDVAARGLDISGVTHVVNYDIPNSPDVYVHRIGRTGRVG
                     RSGRAITLITPRQRKSLEEIEHHAKTEIREWSPNGKPAEPEAAAEPAPEPEEEVRRPR
                     HTKPRREVSEPLAKLIVSGGRSHGLEPADVVSAVVDATHLDGEDVRDVRVLERFSFVH
                     VPEARAEEVASKVNGTNVRGVELRAEVVSR"
     gene            2174..2635
                     /locus_tag="VF752_13870"
     CDS             2174..2635
                     /locus_tag="VF752_13870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8856680.1"
                     /translation="MSKATIHTNHGAIEVDLFDEDAPKTVENFRKLAGDGFYEGVIFH
                     RVIPDFMIQGGDPTGTGTGGPGYTFEDEFNQHKVVRGALAMANAGPNTNGSQFFIVTT
                     QEAPWLDGKHTVFGEVTGGMESVDAIEQTPTDSRDKPVEDAVIERVELAAP"
     gene            2861..4399
                     /locus_tag="VF752_13875"
     CDS             2861..4399
                     /locus_tag="VF752_13875"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX8856681.1"
                     /translation="MSTLDTTTDVTGSNGHIDEAAIPVENPATGETIAHVPDMGADEV
                     HEVVERARRAQPAWNALGFEGRARVMRKARRWLIQNRERMINTIVAETGKTPEDAQLT
                     EVFFVADSLGFWAKNAEKYLADERIRSHSPTLLGRKLVKRYKPRGVIGIIGPWNYPMA
                     LPFGDTIPALMAGNTAVVKPSEVTPLSMLLMAEGFRAAGAPDDVLLIATGRGETGAAL
                     IDHVDMVAFTGSTATGKKVAARAAERLIPTTLELGGKDPMIVLSDADLERAANTAVYW
                     GMSNGGQICQAVERVYVEEPVYDEFVSKVVEKASALRQGPPGEIGSVDIGAVTFPPQI
                     ELIERHVNDAVDKGAKVLVGGKRREGGGRFFEPTVLVDVDHTMAIMRDETFGPALPIM
                     KVRDVEQAVELANDTRYGLNSSVWTKDVEKGRRIAERIDAGSACVNDAVINYGAQELP
                     FGGVKESGIGTRHSGSGIQKYCNTQALLITRFGMKREPFYFPYSKRTTKMLERVLVLM
                     YGRK"
BASE COUNT          796 a         1552 c         1589 g          632 t
ORIGIN      
        1 cggcgcctac gccgagtacg cggtcgcgaa cccgatcggc accttcccca tcccggatgg
       61 cgtgggcgac ggcgtggcgc tcgcgctgct gatccagggc ctcaccgcct ggcaccttta
      121 tcgcacgtcc gcgaagctcc agcctggcga gaccgtggtg gtgcatgccg ccgccggagg
      181 tgtgggcaac ctcgccgtgc agctcggcaa ggcgatgggc gcggggcggg tgatcgccac
      241 cgcgagttcc gaggacaagc gccaagccgc cctcgagatc ggcgccgacg ccgccgtcga
      301 cgtcacgcgc gcggaccttg gcgacgccct ccgcgaggcc aacggcggca acaaggtcga
      361 cgtggtgctc gagatggcgg gcggccgcgt gttcgacgag agcctctcgg tgctcgcccc
      421 cttcggccgc ctggtgacct acggcatcgc gagccgcgag ccaaaccagg tgtcgagcgg
      481 cacgctcatg cgcaagtcgc aggccgtcgt cggcttctgg ctgttccacc tgttccagcg
      541 cccagagatg ctgcgcgagc cgctcgccga cctgttcgag cgcgccgctc gcggggagct
      601 gaagccgctg gtgggcacga catatccgct ctccgacgtg gcccgcgccc acgaggacat
      661 gcaggcgcgc cgcacgagcg gcaagctgct gatcgacccg tctgcgtgac caccctgagt
      721 accctccctc ggcgatgacg accttcgccg acctcggcct gtccgaagtg accctcgagg
      781 cactcgcgca cctcggctac gaacgcccga cgccgattca ggagcaggcg atccccgagc
      841 tgctcgcggg ccacgacgtg atcggccagg cccagaccgg caccggcaag accgccgcgt
      901 tcggcctgcc gctgatcgag tacgtggacc cctcgctcga ggagatccag gcgctcgtgc
      961 tcactccgac gcgcgagctg tgcatccagg tgacccaggc gctccgcgcc tacggcgagc
     1021 gcaagggagt gaacgtggtg gccacgttcg gcggcgcgcc gatccgcggc caggtggcgc
     1081 agatcaaggg cgcgcacgtg gcggtcggca ccgtcgggcg ggtgatggac ctgatgaacc
     1141 gccatgagct ggtgctcacc gacacgcgct acgtggtgct cgacgaggcc gacgagatgc
     1201 tcgacctcgg cttcttcgag gacgtcgaga cgatcctgtc gcgcgcgccg tccgggcgcc
     1261 agactgcgct cttctcggcc acgattccgc cgccgatcaa ggagctcgcc gagaagcgca
     1321 tgttcgaccc gatcacgatc aaggtccgcg cggcgcagct aacgatcgac acggtggagc
     1381 agttctatct cgaggtcccg gaccgcgaga aggccgatac gctcgcgcag gttctaaagg
     1441 cggagcgtcc cgatcaagcc atcgtgttcg tgcgcacgaa gatcggtgcc gaccgactgg
     1501 cgcgctcgct gaacgaccgc ggcgtgcgcg tgaaggcgct ccacggcgac atgtcccagg
     1561 gttcgcgcga cggcgtcatg ctcgcgttca aggggggccg ggaccggctg ctcgtcgcga
     1621 ccgacgtcgc cgcgcgcggg ctcgacatct cgggcgtcac gcacgtcgtg aactacgaca
     1681 tcccgaactc gcctgacgtc tacgtgcacc gcatcggccg cacgggccgc gtgggtcgca
     1741 gcgggcgtgc gatcacgctg atcacgcccc gccagcgcaa gagcctcgag gagatcgagc
     1801 accacgcgaa gacagagatc cgcgagtggt cgccgaacgg caagccggcc gagcccgagg
     1861 cggccgcgga gccggcgccc gagccggaag aggaggtgcg ccggccgcgc cacaccaagc
     1921 cgcgccgcga ggtctcggag ccgctcgcca agctgatcgt gtcgggaggg cgaagccacg
     1981 gcctggagcc cgccgacgtg gtgagcgcgg tcgtggacgc aactcatctc gacggcgagg
     2041 atgtacgtga cgtccgcgtc ctcgagcggt tcagcttcgt gcatgtgccc gaggcccgcg
     2101 ccgaggaggt tgcgagcaag gtgaacggaa cgaacgtcag gggcgttgag ctccgcgcgg
     2161 aggtggtaag cagatgagca aggcgacgat ccacacgaac cacggcgcca tcgaggtcga
     2221 cctattcgac gaggacgcgc cgaaaacggt cgagaacttc cggaagctcg ccggcgacgg
     2281 cttctacgag ggcgtgatct tccaccgggt gatccccgac ttcatgatcc agggcggcga
     2341 tccgaccggc accggcaccg gcggtcccgg ctacacgttc gaggacgagt tcaaccagca
     2401 caaggtcgtg cgcggtgcac tggcgatggc caacgccgga ccgaacacga atggctcgca
     2461 gttcttcatc gtcacgacgc aggaggcgcc gtggctcgac ggcaagcaca ccgtgttcgg
     2521 cgaggtgacc ggcgggatgg agtcggtgga cgcgatcgag cagacgccca cagacagtcg
     2581 cgacaagccg gtcgaggacg ccgtcatcga gcgggtggag ttggcggcac cctagtccgc
     2641 ctcgggcgag cgctgcgcgg tgccaatacc gctcccgctc aggagaaagg actcctgccg
     2701 ctaagtcctg actttggttc tgatatgcgg aaccaaactc aggagttgga gcccgacacc
     2761 gacgatcact cagtgtcttt gggctcccgt cgaagctgat ttggctgggc ggccaaacgg
     2821 tttagactcg cgccgggcac cgaccgaggg agagacacgc atgtccacgc tcgacacgac
     2881 gaccgacgtc acaggcagca acggccacat cgatgaggcc gcgattccgg tcgagaaccc
     2941 ggccacgggc gagaccatcg cccacgtgcc cgacatgggc gccgacgagg tgcatgaggt
     3001 agtggagcgc gcgcgccgcg cccagcccgc ctggaacgcg ctcggcttcg agggccgcgc
     3061 gcgcgtgatg cgcaaggccc gccgctggct gatccagaac cgcgagcgca tgatcaacac
     3121 gatcgtcgcc gagaccggca agacgccgga ggacgcgcag ctcaccgagg tgttcttcgt
     3181 cgccgactcg ctcggcttct gggctaagaa cgccgagaag tatctcgccg acgagcgcat
     3241 ccgctcgcac tcacccacgc ttctcggccg caagctcgtg aagcgctaca agccgcgcgg
     3301 cgtgatcggg atcatcggcc cctggaacta cccgatggcc ctgccgttcg gcgacacgat
     3361 cccggcgctc atggccggca acaccgcggt cgtgaagccg agcgaggtga cccccctctc
     3421 gatgctcttg atggccgagg gcttccgcgc ggccggcgcc cccgacgacg tgctgctgat
     3481 cgccaccggc cgaggagaaa ccggcgcggc gctgatcgac cacgtggaca tggtcgcctt
     3541 cacgggctcg accgccacgg gcaagaaggt cgccgcgcgg gcggccgagc gactcatccc
     3601 caccacgctc gagctcggcg gcaaggaccc gatgatcgtg ctgagcgatg ccgatctcga
     3661 gcgcgccgcc aacaccgccg tgtactgggg catgtcgaac ggcggccaga tctgccaggc
     3721 cgtggagcgc gtttacgtgg aggagccggt ctacgacgag ttcgtgtcca aggtcgtcga
     3781 gaaggcgagc gcgctgcgcc agggtccgcc aggcgagatc ggctcggtcg acatcggcgc
     3841 ggtcaccttc ccgccgcaga tcgagctgat cgagcgtcac gtgaacgacg cggtcgacaa
     3901 gggcgcgaag gtgctcgtgg gagggaagcg ccgcgagggc ggcgggcgct tcttcgagcc
     3961 gaccgtgctc gtcgacgtcg atcacacgat ggcgatcatg cgcgacgaga cgttcggtcc
     4021 cgcgctcccg atcatgaagg tgcgcgacgt ggagcaggcg gtcgagctcg cgaacgacac
     4081 ccgctatggc ctcaactcga gcgtctggac gaaggacgtg gagaagggcc gcaggatcgc
     4141 cgagcggatc gacgccggct cggcgtgcgt gaacgacgcc gtaatcaact acggagcgca
     4201 ggagctgccg ttcggcggcg tcaaggagtc cggcatcggc acgcgccaca gcggctcggg
     4261 catccagaag tactgcaaca cacaggcgct gctgatcacg cgcttcggga tgaagcgcga
     4321 gccgttctac ttcccgtact cgaagcggac gaccaagatg ctcgagcgcg tgctggtgtt
     4381 gatgtacggg cggaagtagg acaacggcgc atctagccga cggggtgcgg gtagcagggc
     4441 cgggatgcaa tccggcgagc gagcccgcac cgcttggtgg cgccgaggcg cgatctacca
     4501 gatctacccg cgctccttcg ccgactcgaa cggcgacggc gtgggcgacc tgaagggcat
     4561 caccgctca
//
LOCUS       DASVXS010000245         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_211172, whole genome shotgun sequence.
ACCESSION   DASVXS010000245 DASVXS010000000
VERSION     DASVXS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211172"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2454)
                     /gene="secD"
                     /locus_tag="VF752_13880"
     CDS             complement(<1..2454)
                     /gene="secD"
                     /locus_tag="VF752_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX8856682.1"
                     /translation="MSNPRRNFFVVLFMLGLIAVSAGIIATKQTRLGLDLKGGVELVY
                     EGQPTPAVPKVTPQAIDDAISTIRKRTDVLGVSEPEITRSAGNQITIGLPDVKNVDRA
                     EQQVGSTAQLQFYDWEPNVYQGPNKLVDPNTASTAAAANNPIPNLFQAVKLASESKPR
                     AEATDVPAGGPNVAELQQHGIDPNNAQAVRQYYDNKNDTTGDKYYLFGPGVGLDRQLI
                     TGPDTSCGSLLSDYTDKLVPGQKTTPQKVTKDSQCADQLAKLGTAGPPKGSIVMKVPK
                     GVIVIQAERPRTLAKNVPFNQFFVIEDDSELSGSDIKNPKQTTDPQTGEPIVTMEFTD
                     KGRKQFAAATKREAQRGAQQIPQPGQPQDSTFQRFAITLDNQIISLATINWRENPEGI
                     DGRTGAQINGIGNIQETQDLAENLRIGALPIDLKLISKTQVSSTLGKQALHQGLIAGA
                     VGLALTLIFLVSFYRVLGLVAALALLVYAVILFALVKLIPITLTLPGIAGLILTLGVA
                     ADANIVIFERIKEEVRGGSSIPRAISNGYTKALRTIIDANVVTIGVAFILFMLATAGI
                     KGFAFTLLVGTLVSLFTAVLATSAILGSLARTRLMRSKHMLAAGKERIRWHFDFMGKS
                     KWFFSMSGTILVAGALAIAGLGINFGIDFESGTRITTPVERKASVQQIRNVLSPLGYG
                     DAKIQSVQNSSLGPNVFQITVKKLQPDKVNVVQQKLDQNFGVARADFTADSVGPTFGA
                     QIAKTAVIAIIASLVLISLYIGLRFEFKFAVPVLIALAHDLLITTGVYALFQREVTAD
                     TVAALLTILGYSLYDTIIVF"
     gene            complement(2464..2919)
                     /locus_tag="VF752_13885"
     CDS             complement(2464..2919)
                     /locus_tag="VF752_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8856683.1"
                     /translation="MLTGMAHFICPNCGNRSTSNDRTAGFSQRPRGCSKCGFSFLFEM
                     LDDYYPAPNAAFVILDQKANVIGLGRGVRELTGLGDEDAVGRPVRDVFGLEFQNGDDH
                     IGTALEWGVRVLDKPVTVHAEGDRPEPAKADIFPAYDDDGGLLLVLTPS"
     gene            complement(2966..3388)
                     /locus_tag="VF752_13890"
     CDS             complement(2966..3388)
                     /locus_tag="VF752_13890"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8856684.1"
                     /translation="MANELGPQDSVGAEQVRAAGGVLVRDGLVLLVHRPKYDDWSLPK
                     GKLDPDESWEDAAVREVYEETGWRTHLGRFLTETHYNDRKGRPKTVRWWEMEPLEHTG
                     FEPNDEIDDLRWVPLSEAPDALQYDTDRAVLEALAREP"
     gene            complement(3411..4058)
                     /locus_tag="VF752_13895"
     CDS             complement(3411..4058)
                     /locus_tag="VF752_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856685.1"
                     /translation="MAEDFRAVLVVEGEHEGHRLAGRLGRWDAEDELEGQLPDRVVVS
                     HDGPELFLYANTREELDRAIEAAQRVLASSRVERVERWHDAEESWEDADRPLPQTEDE
                     IEEEQRRLAERERAEAERRGEVDWEVRAELPSRRLAEELEEWLDAQGHPAVRRWKYVF
                     VPVPSEEDGRELAELILHSDAGEAASVDVEGTGAATARGRPFGGNVFAIFGGLGN"
     gene            complement(4094..5140)
                     /gene="ruvB"
                     /locus_tag="VF752_13900"
     CDS             complement(4094..5140)
                     /gene="ruvB"
                     /locus_tag="VF752_13900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8856686.1"
                     /translation="MSGGIRTPGVDRIQAAAALGGDEELDRSLRPRRLADFVGQSAVK
                     EQLGVFIEAANARGEALDHVLLAGPPGLGKTSLAQIVASELGVPFVQTAGPALERKGD
                     VAAFLTALEPRSVFFVDEIHRLPRALEETFYPAMEDRKLPITVGQGAGARVVTLDLPQ
                     FTLVGATTRAGLLTTPLRDRFGVSHRLDLYTPEDLAEIVLRSAGILEVGIDRPGAEAI
                     ARRSRGTPRVANRLLKRVRDYAEVRGAGHIDQGVAEEALDLLEVDHAGLDRLDRDILE
                     TICVKFEGGPVGLSTLAVAVGEEADTIEDVYEPYLLQQGFIKRTPRGRVATGAAYEHL
                     GLERPAEAGPASLF"
     gene            complement(5137..5715)
                     /gene="ruvA"
                     /locus_tag="VF752_13905"
     CDS             complement(5137..5715)
                     /gene="ruvA"
                     /locus_tag="VF752_13905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8856687.1"
                     /translation="MIASVRGTVSVRRPDHVVIECNGVGYRLAVSAETLKHVPAAGNE
                     AFLHTHLVTRDDGMYLFGFASEEERELFHLLNGVQGVGPKVSLAVLSGGTTRELMRAI
                     AAGDSARFQAVPGIGKRTAERIIVELREKVGGEASAPDEIVITKSDDPRTLARQGLVG
                     LGFSVQEADELLDKAEGDTPEDLIGAALRAAR"
     gene            complement(5712..6203)
                     /gene="ruvC"
                     /locus_tag="VF752_13910"
     CDS             complement(5712..6203)
                     /gene="ruvC"
                     /locus_tag="VF752_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX8856688.1"
                     /translation="MVVVLGIDPGTANTGFGVVLARAGSLAALDGGVIDTEANMPLER
                     RLARIHDGVCALIEEHAPEALAVEDLYFGQNARSAFAVGQARGVVLLAAGQAGIPSFS
                     YTPQAVKQAVCGAGNAAKDQVQRMVGALLRLPEPPHPDHAADALAVAICHANGANLKA
                     ALA"
     gene            6488..>6912
                     /locus_tag="VF752_13915"
     CDS             6488..>6912
                     /locus_tag="VF752_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX8856689.1"
                     /translation="MNAVLVGFGLGFVVAAQVGPISLLLIRSVMRGAFVSGLAIGAGA
                     AIIDTTYASLGALGAGRLLSIEALRLTLGIAGALVLAYFALRTLRDAFRVRIGGELPA
                     EIASPRRALATSLAATASNPLTIVSWAAAFSAASTAAAA"
BASE COUNT          972 a         2383 c         2405 g         1152 t
ORIGIN      
        1 gaacacgatg atcgtgtcgt agagcgaata accgaggatc gtcagaagcg ccgcgacagt
       61 gtcggccgtg acctcgcgct ggaagagcgc gtacacgccg gtggtgatca ggaggtcgtg
      121 cgccagcgcg atcagcaccg gcaccgcgaa cttgaactcg aacctgagcc cgatgtacag
      181 ggagatcagg acgagcgacg cgatgatcgc gatcaccgcc gtcttcgcga tctgggcgcc
      241 gaaggtcggg ccgaccgagt cggccgtgaa gtccgcgcgg gcgacgccga agttctggtc
      301 gagcttctgc tggaccacgt tcaccttgtc cggctgaagc ttcttgaccg tgatctggaa
      361 gacgttcggg ccgagggacg agttctggac cgactggatc tttgcgtcgc catagcccag
      421 cgggctgagc acgttgcgga tctgctggac gctggccttg cgctcaaccg gggtggtgat
      481 gcgggtgccc gactcgaagt cgatgccgaa gttgatccca aggcccgcga tcgcgagcgc
      541 gccggcgacg aggatcgtgc cggacatcga gaagaaccac ttcgacttgc ccatgaagtc
      601 gaagtgccag cggatgcgct ccttgcccgc ggccagcatg tgcttcgagc gcatgaggcg
      661 cgtgcgcgcc agcgagccga ggatcgcgga cgtggcgagc acggccgtga acagcgacac
      721 gagcgtgccg acgagcagcg tgaacgcgaa gcccttgatg cccgcggtgg cgagcatgaa
      781 gaggatgaag gcgacgccga tcgtgacgac gttcgcgtcg atgatcgtgc ggagcgcctt
      841 ggtgtagccg ttgctgatcg ccctcggtat ggacgagccg ccgcgcacct cttcctttat
      901 tcgctcgaag atgacgatgt tcgcgtcagc ggccaccccc agcgtgagga tcaggcccgc
      961 gatgccaggc agcgtgagcg tgatcggtat gagcttgacg agggcgaaca ggatcaccgc
     1021 gtagacgagc agcgccaacg cggccacgag cccgagcacg cggtagaagc tgaccaggaa
     1081 gatcagcgtg agcgcgagac cgaccgcgcc cgcgatcagg ccctggtgga gcgcctgctt
     1141 gccgagcgtc gaggagacct gcgtcttcga gatcagcttc aggtcgatcg gcagcgcccc
     1201 gatgcgcagg ttctcggcga ggtcctgcgt ctcctggatg ttgccgatgc cgttgatctg
     1261 cgcgccggtg cggccgtcga tgccctccgg gttctcgcgc cagttgatcg tggcgagcga
     1321 gatgatctgg ttgtccagcg tgatcgcgaa gcgctggaag gtcgagtcct gcggctggcc
     1381 cggctgcggg atctgctggg cgccgcgctg cgcctcgcgc ttggtggcgg cggcgaactg
     1441 cttgcggccc ttgtcggtga actccatcgt cacgatgggc tcgcccgtct gcgggtcggt
     1501 cgtctgcttc gggttcttga tgtccgagcc ggacagctcg gagtcgtcct cgatcacgaa
     1561 gaactggttg aaggggacgt tcttggcgag cgtccgcggg cgctcggcct ggatcacgat
     1621 cacgcccttc ggcaccttca tcacgatgct gcccttgggc gggccggccg tgccgagctt
     1681 ggcgagctgg tcggcgcact ggctgtcctt cgtgaccttc tgcggcgtgg tcttctggcc
     1741 ggggacgagc ttgtccgtgt agtcggagag gagcgagcca cagctggtgt cgggccccgt
     1801 gatcagctgg cggtcgaggc ccacgcccgg gccgaagagg tagtacttgt cgcccgtcgt
     1861 gtcgttcttg ttgtcgtagt actggcggac ggcctgcgca ttgttcgggt cgatcccatg
     1921 ctgctgcagc tccgcgacgt tcgggccgcc ggcgggcacg tccgtcgcct ccgcgcgcgg
     1981 cttcgactcc gacgcgagct tcaccgcctg gaagaggttg ggaatcgggt tgttggcggc
     2041 cgccgcggtc gacgccgtgt tcgggtcgac cagcttgttc ggcccctggt agacgttggg
     2101 ctcccagtcg tagaactgga gctgcgcggt cgagccgacc tgctgctcgg cgcgatcgac
     2161 gttcttcacg tcgggaaggc cgatcgtgat ctggttcccc gccgagcgcg tgatctcggg
     2221 ctccgacacg ccgagcacgt cggtgcgctt gcggatcgtg gagatcgcgt cgtcgatggc
     2281 ctgcggcgtg accttcggaa ccgccggcgt cggctgcccc tcgtaaacca gctccacgcc
     2341 gcccttcaga tcgagtccga gccgggtctg cttcgtcgcg atgatcccgg ccgagaccgc
     2401 gatcaagccc agcatgaata ggactacgaa gaagttccgg cgcggattgc tcaagtttgc
     2461 gttttagctc ggggtgagga cgagcagcag gccgccgtcg tcgtcgtacg cggggaagat
     2521 gtcggccttc gccggctctg gccgatcgcc ttccgcgtgc acggtcaccg gcttgtcgag
     2581 cactcgcact ccccactcga gcgccgtgcc gatgtgatcg tccccgttct ggaactccag
     2641 gccgaacacg tcccggacgg gccggcccac cgcgtcctcg tcacccagtc ctgtgagttc
     2701 ccgcactccc cggccgagcc cgatcacatt cgccttctga tcgaggatca cgaacgccgc
     2761 gttgggcgcc gggtagtagt cgtcgagcat ctcgaacagg aagctgaagc cgcatttgga
     2821 gcagcctctc ggccgctggc tgaagccggc ggtgcgatcg ttcgaggttg agcggttgcc
     2881 gcagttcggg cagatgaagt gggccatgcc agtcagcaca gattaggcgc tcccctgtta
     2941 agcgcccagt gtcctcagct tacccttagg gctctcgcgc cagtgcctcg agcacggcac
     3001 gatcggtgtc gtactggagg gcgtcgggcg cttcgctgag cggcacccag cgcagatcgt
     3061 cgatctcgtc gttcggctcg aagccggtgt gctcgagcgg ctccatctcc caccagcgca
     3121 cggtcttcgg gcgccccttg cggtcgttgt agtgggtttc ggtgaggaag cggccgaggt
     3181 gcgtgcgcca gcccgtttct tcgtagacct cgcgcacggc ggcgtcttcc cagctctcgt
     3241 cgggatcgag cttgcccttg gggaggctcc agtcgtcgta cttggggcga tggacgagga
     3301 gtaccaggcc gtcgcggacc agaactccgc cggccgcgcg gacctgctcg gcccctacgg
     3361 aatcttgagg gcccagttcg tttgccaacc cggacctcga gattccgtct ttagttaccg
     3421 aggccgccga aaatcgcgaa tacgttgccg ccgaagggtc gaccgcgcgc ggtggcggcg
     3481 ccggtgccct cgacgtccac tgaggcggcc tcaccggcgt cggaatgcag gatgagctcc
     3541 gccagctcgc ggccgtcctc ttcgctcggc accggcacga agacgtactt ccagcgacga
     3601 acggccggat gaccctgcgc gtcgagccat tcctcgagct cctccgccag acggcgactg
     3661 ggcagctctg cgcgcacttc ccagtccacc tcgccgcggc gctcggcctc tgcgcgctcg
     3721 cgctcggcca ggcgccgctg ctcctcctcg atctcgtcct ccgtctgcgg gagcgggcga
     3781 tcggcgtcct cccagctctc ctcggcgtcg tgccagcgct cgacgcgctc gaccctcgag
     3841 ctggcgagca cgcgctgtgc ggcctcgatc gcgcggtcga gctcctcgcg cgtgttggcg
     3901 tagaggaaga gctcgggacc gtcgtggctc accaccaccc ggtcgggaag ctggccctcg
     3961 agctcgtcct ccgcgtccca gcgcccgagc cggccggcga gccggtggcc ctcgtgttcg
     4021 ccctcgacga cgagtaccgc tctgaagtct tcggccatcg gcgagcacct tactcgcccc
     4081 gagcttgctc ggatcagaag aggctcgccg gcccggcctc ggccgggcgc tcgagcccca
     4141 gatgctcgta ggccgcgccg gtcgcgacgc ggccgcgcgg cgtgcgcttg atgaagccct
     4201 gctgcagcag gtagggctcg tacacgtcct cgatcgtgtc ggcctcctcg cccacggcga
     4261 ccgccagcgt ggagagcccg accggcccgc cctcgaactt cacgcagatc gtctcgagga
     4321 tgtcgcggtc gaggcggtcg aggccggcgt ggtcgacctc gagcaggtcg agcgcctcct
     4381 cggccacgcc ctggtcgatg tggcccgcgc cgcgcacctc ggcgtagtcg cgcacccgct
     4441 tgagcaggcg gttggccacg cgcggtgtac cgcgcgagcg gcgcgcgatc gcctccgcgc
     4501 cgggccggtc gatgcccacc tcgaggatgc cggccgagcg caggacgatc tcggccagat
     4561 cctcgggcgt gtacaggtcg agccgatgcg agacaccgaa gcggtcgcgc aggggcgtag
     4621 tgagcaatcc cgcgcgggtg gtggcgccga ccagcgtgaa ctgcggcagg tcgagcgtga
     4681 ccacgcgcgc gcccgccccc tggcccaccg tgatcggcag cttgcggtcc tccatcgcgg
     4741 gatagaacgt ctcctcgagc gcgcgcggga ggcggtggat ctcgtcgacg aagaagactg
     4801 agcgcggctc gagcgccgtg aggaaagcgg cgacgtcgcc cttgcgctcg agcgccgggc
     4861 ccgcggtctg cacgaacggc acgccgagct ccgaggccac gatctgcgcg agcgaggtct
     4921 tgccgaggcc cggcggcccg gcgagcagca cgtgatcgag cgcctcgccg cgcgcgttcg
     4981 ccgcctcgat gaacacgccg agctgctcct tgaccgcgct ctggccgacg aagtcggcga
     5041 gcctgcgcgg gcgcagcgag cggtcgagct cttcgtcgcc ccctagggcg gcggccgcct
     5101 ggatgcggtc gacgcccggc gtgcggatgc cgccgctcac cgggccgccc tcagcgcagc
     5161 gccgatcagg tcctcgggcg tgtcgccctc ggccttgtcg agcagctcgt ccgcctcctg
     5221 cacgctgaac ccgagcccga ccaggccctg tcgcgcgagc gtgcgcgggt cgtcgctctt
     5281 cgtgatcacg atctcgtcgg gagcggaggc ctcgccccca accttctcgc ggagctcgac
     5341 gatgatgcgc tccgccgtgc gcttgccgat cccgggcacg gcttggaagc gcgccgagtc
     5401 gcctgcggcg atggcgcgca tcagctcgcg ggtcgtgcca ccggacagca cagcgagcga
     5461 caccttcggc cccaccccct gcacgccgtt cagcaggtgg aagagctcgc gttcctcctc
     5521 gctcgcgaag ccgaacaggt acatgccgtc gtcgcgcgtg acgaggtggg tatggaggaa
     5581 cgcctcgttg ccagcggccg gcacgtgctt gagcgtctcg gccgagacgg cgagccggta
     5641 gccgactccg ttgcactcga tcaccacgtg gtccggcctg cgcacgctca cggtgccgcg
     5701 caccgacgcg atcacgcgag cgccgccttc aaattggccc cgttcgcgtg gcagatggcg
     5761 accgccagcg cgtcggccgc gtggtcggga tgcggcggct caggcaaccg aagcagcgca
     5821 ccgaccatgc gctggacctg gtccttggcg gcgttgcccg cgccgcagac cgcctgcttg
     5881 accgcctggg gcgtgtacga gaaggaggga atcccggcct ggcccgcggc gagcaggacc
     5941 actccgcgcg cctggccgac cgcgaacgct gagcgcgcgt tctgcccgaa gtagagatct
     6001 tcgaccgcga gcgcttccgg cgcgtgctcc tcgatgaggg cgcagacgcc gtcgtggatg
     6061 cgtgccagcc ggcgctcgag aggcatgttg gcctctgtgt cgatcacgcc gccgtcgaga
     6121 gccgcgaggc tacccgcgcg cgcgagcacc acgccgaagc ccgtattcgc cgtgccggga
     6181 tcgatgccga gaaccaccac cacgactggg attctgcgcg cgcggccgga cgcctcccgg
     6241 cgtccgcccc gccctagtcc ctggccgggg cggagcggac gggcaccaag cggtaacgcc
     6301 tcacgaccgg agagggatga gattcagccg tgttgcctgc cggcaccagc acccaacgac
     6361 ggcttggggt gtccgggagc ttgttcttca tggccgagat gatcggcgcc cgccggcggg
     6421 gcgccattgt cccggtacac aaagttgccg gattcgaccc ctccagcatg tcgggtacgc
     6481 tcgccccatg aacgccgtac tcgtgggatt cggcctcgga ttcgtggtgg cggcccaggt
     6541 cgggccgatc tcgctgctgc tgatccgctc agtgatgcgg ggcgcgttcg tgagcggcct
     6601 ggcaatcggc gcgggcgcgg cgatcatcga caccacctac gcgtcactgg gcgcgctcgg
     6661 cgccgggcgc ctgctgtcga tcgaggctct gcggctgacg ctcggtatcg ccggcgcgct
     6721 ggtgctcgcg tacttcgcgc tgcgcacgct gcgcgacgcg ttccgcgtgc gcatcggcgg
     6781 ggagcttccc gcggagatcg cctcgccgcg gcgcgcgctc gcgacctcgc tggccgccac
     6841 ggcgtccaac ccgctcacga tcgtgtcgtg ggccgccgcc ttctctgccg cctcgaccgc
     6901 cgccgcggcc ca
//
LOCUS       DASVXS010000246        10184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1342024, whole genome shotgun sequence.
ACCESSION   DASVXS010000246 DASVXS010000000
VERSION     DASVXS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342024"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /gene="rsmA"
                     /locus_tag="VF752_13920"
     CDS             <1..806
                     /gene="rsmA"
                     /locus_tag="VF752_13920"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX8856690.1"
                     /translation="LRRLREFGVRPNRELGQNFLIDDNVLGVIGREAALGQADVVLEV
                     GGGLGVLSEYIAPRVSYLHVVEVDESLRPPLEQALEPYPNTTLHIADAVQLDFAALEP
                     APTKVVANLPYGVAATVILKALEELPSAQRVVAMVQREVGERLAAAPGSKTYGATSVL
                     AQLSARVRVARRISANVFFPVPNVESAIVVLERAAPPPRPELASVVAAAFAHRRKALA
                     RSLALAPGAAPGIREKAREALESMGLPADARAERLSPEQFDELERRLAA"
     gene            803..1639
                     /locus_tag="VF752_13925"
     CDS             803..1639
                     /locus_tag="VF752_13925"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856691.1"
                     /translation="MTQREVAPAKVNLLLHVGPPREAGLHELCSLFASIDLADELGFE
                     AAPALAADEVVCPGVDGRNLIAVALAEFRERVDSALPPLRVVVEKRIPVAAGLAGGSA
                     DAAAALRAANAIAGEPATTDELRALAARLGSDVPSQVEPRHALVMGAGEQVEPLELPG
                     LALVLVPYADGLSAAEVYAEADRLGSTRERLDPEGARALARLPAQELAARLESDLEPA
                     VLSLRPGVRDNLDALLEAGARAALVSGSGPTAFGVFPNDEEARRAAGLIDGAIQARLR
                     TA"
     gene            1636..3204
                     /locus_tag="VF752_13930"
     CDS             1636..3204
                     /locus_tag="VF752_13930"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HEX8856692.1"
                     /translation="MSTKHVIELVIAGVLLVAALWLRHKRRLSGERFVVVLVVAAILA
                     VIGSGVLSGFNAEHAITDLAHALGKWTYALVGVLAFAETGAFIGFIAPGEFTVILGGV
                     IAGEGTINIFLLITLVWVCAFLGDTVSFFIGRRLGREFLLKHGERFKVTHERLEQVER
                     FFHRHGGATILIGRFVGFVRPLAPFIAGSSRLPYRRFLPYSVLGTGLWGSTLCVLGYI
                     FYRSFSKITSIAGRATLIFSILVGLIAAVVWISKRLRDPEQRRALERWADNHAVVRPL
                     WHHVIRPAYRVLAPQVRFVWRRLTPGGLGIEFTSALAVSVVGLYLFTMYLLAITSDPG
                     PTAADEFFQDLASDTRTHALVQLAKVISVFGSLPVTGGLVLAGALALAWRRRPVEMLA
                     LLSGALTIWIGVQATKAGVDRPRPEPQLVNTTGSAYPSGHAAYSTVYIALAVIGWRVL
                     PGLATKTFVILIAALISVAIGLSRIYLNVHWWSDVVGGWGLGCGIFGLSACVALVVHH
                     LRNTRSAEPLPEPS"
     gene            3208..3432
                     /locus_tag="VF752_13935"
     CDS             3208..3432
                     /locus_tag="VF752_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856693.1"
                     /translation="MGSISSTTLVLIAAGAVGLVAYFTLVLAPAWSSYGRLWERLAAS
                     FLSLYILATFIGVGLGIGFAVVWFYDRYAG"
     gene            3464..4729
                     /locus_tag="VF752_13940"
     CDS             3464..4729
                     /locus_tag="VF752_13940"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8856694.1"
                     /translation="MSTVEGGKTPANSGFMDSFSDVSEAVESGAGLPEVARAAGRALG
                     ASVAIVDSSSSVVAVACASPADERAVLAREAGVEVLDLRVAESRVGELRYRLRGETPP
                     APLLRMVGTLIALEVERSRGPERASEAAVGAFLADLLDRRATDRENILARATELGADL
                     AQGGSVVVARAHPLRPAEGDWRARAQSVAERGARGVARDTLAATVALEAAAQSVPNGP
                     GELVLVVPGADADLSRRVATAVLRELEGGMPGFAITVARSRPAGDPVDLHRAGAEAVL
                     AANVAEARGAQELAFEETGSYRLLLPAMIDDPAELRRFHEETIAPLVGYDEQYETELV
                     RTLETFLDADGNVGQTAERLFTHRHTIRYRLERVKELTGLDVSSTDGRERLGLGLKAM
                     RVLGIMPTRGPADEAGTEAGQVPRSEADI"
     regulatory      4771..4921
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4925..5680
                     /locus_tag="VF752_13945"
     CDS             4925..5680
                     /locus_tag="VF752_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8856695.1"
                     /translation="MKHARLGMTTAACMMAALAALAPTASAGVRFGQRTLHMGSRGHD
                     VKVLQSWLSHLGISTSIDGVFGPGTRSKLHRFEKANAGFTADGRLTRSEAQRLRKLIE
                     AKFGAPTPVAPPASKAQLSSDGRTAVAPADAPQPVKDAVAAANQITRKPYRYGGGHGS
                     FEDSGYDCSGAVSYALHGAGLLRAPLDSSGFYSWGKSGRGSWITVYTKSSHAYVVIAG
                     LRFDTSGSGESGPRWRTEARSSAGYAARHPSGL"
     gene            complement(5749..5820)
                     /locus_tag="VF752_13950"
     tRNA            complement(5749..5820)
                     /locus_tag="VF752_13950"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5785..5787),aa:Gln,seq:ttg)
     gene            5870..7246
                     /gene="glmU"
                     /locus_tag="VF752_13955"
     CDS             5870..7246
                     /gene="glmU"
                     /locus_tag="VF752_13955"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX8856696.1"
                     /translation="MASPTVLIMAAGHGTRMHSSVPKVLHPICGRPMLHWVIESARAA
                     GAGKIVAIVRPGEGVADALPDGVVAAEQTDGEGTGCAVLASRDAVGGSGTVVVLSGDH
                     PLVSAELIREFVVAHEREGAAATMLTSEELDPTGYGRVIRANDGSVERIVETKHPEGL
                     SDEELATREINMGAYAFDAEELFSALDEAGEQYGERYLTDVFPILRERGRRIAAHRTS
                     DVSSAMGVNTRADLMAVAERLQRRLLDEHARAGVTFELPGSVYLEAGVEIGPDTVIAP
                     GVTLRGTTRIGGGSTIGPNTTVIDSEIGDGVTALHAYLLEAQVENGVSIGPFAYLRPG
                     TVVREGAKIGTYVEVKNSNIGRGTKVPHLSYLGDADVGEETNVAAGNITANYHAGKKT
                     RTRIGDHVHTGVHTSLVAPVTVGDDAYTAAGSVITEDVPEGALGISRPPQRNVEGYAE
                     RIRKETKE"
     gene            7243..8274
                     /locus_tag="VF752_13960"
     CDS             7243..8274
                     /locus_tag="VF752_13960"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX8856697.1"
                     /translation="MSTVEVGTRARPTTVPFDYDKRMMVTAGRSNRELAAKIADKLGV
                     ALTDPGLKTFSDGEVYCRYQESIRGADLFIIQSTCGNEREGLTANDALMELLVMVDAA
                     VGASAHRVIAVTPWYGYSRQDKKSAPREPITARLVAKMLETAGIDRLLSLDLHAGQLQ
                     GFFQKPVDHLTAMPVLTQYVKDQLAPDGDLVIIAPDAGRVKLTRKFAQKVGAPYALLE
                     KERPAQQVAEIGYVIGDVKDKCAVIVDDIIDTGGTLAAAAQTVMDEGAKQVYALATHG
                     IFSGNAFETLEQSPIAGIVVTDTVPLRDGAPPLIRQLSCADIVTDSVRAIFTDDSVSE
                     IFAGENQLF"
     gene            8325..8975
                     /locus_tag="VF752_13965"
     CDS             8325..8975
                     /locus_tag="VF752_13965"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8856698.1"
                     /translation="MLDSVAHLVEGNVWGYFVILAVVAVDAVFPLLPSETVIIAGAVL
                     ASNGDMSLTLIILATAAGAMVGDNVCFFLGDKVGEPLARRLFTSPKSRQVYAWGSRMI
                     CDHTWIVSVARFVPCGRTATTFGAGALVVSWRSFIRWDAIGAVVWATYATMVGYLGGE
                     AFQNSLWKPFLLSTAIALAVGFAGELARRRSARRLAREGKLHVSNERRQRAREQLS"
     gene            complement(9072..>10184)
                     /locus_tag="VF752_13970"
     CDS             complement(9072..>10184)
                     /locus_tag="VF752_13970"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX8856699.1"
                     /translation="SSAPPMPWKKVRQVLDEEWDEPVESLFEDFDHEAAAAASIGQVH
                     RAVLPDGRRVAVKIQYPKVADALRADVDNAGVFLRFAKAIAPGLDAKAAARELRERVL
                     EELDYEFEAQNHRAFARGYRGHPFIYVPDVVTRLSRSRVLVTEWVDGVSFEEVKLRPQ
                     PERDRFGEIVFRFCFGSIYHLQQFNADVHPGNYLLMDDDRVAFLDFGMTKTLDDDQIE
                     LEIAAIEAVLSDDAERLRQALADLGFFRSPKRVDAIRLMEQVRAIGGWYMQDRDVTVD
                     SEMVMRAISAASDPRSEFFQLMRRENLPANEMMGRRMETGLLAVLGQLNATRNWNRIG
                     REWWFGDEPATELGREEWAYFEGRGEKRRRRFSERH"
BASE COUNT         1479 a         3578 c         3590 g         1537 t
ORIGIN      
        1 gcctgcggcg gctgcgcgag ttcggcgtca ggccgaaccg cgagctcggc cagaacttcc
       61 tgatcgacga caacgtgctc ggagtgattg ggcgcgaggc cgcgctcgga caagcggacg
      121 tggtgctcga agtaggtgga ggtctggggg tgctcagcga gtacatcgct ccgcgtgtct
      181 cgtacctgca cgtggtggag gtcgacgaga gcctgcgccc gccgctcgag caggcgctcg
      241 agccctaccc gaacaccacg ctccacatcg cggacgccgt gcaactcgac ttcgcggcgc
      301 tcgagccggc gccgaccaag gtcgtcgcga acctgcccta cggcgtggcg gcgaccgtga
      361 tcctcaaggc gctcgaggag ctgccgagcg cgcagcgcgt ggtcgcaatg gtgcagcgcg
      421 aggtgggcga gcggctcgcc gcggcacccg gttcgaagac ctacggcgcc acgtccgtgc
      481 tggcgcagct gtccgcacgc gtgcgcgtcg cgcgccgcat ctcggcgaac gtgttcttcc
      541 cggtgccgaa cgtcgagtcg gcgatcgtgg tgctcgagcg cgcggcgccg ccgccgcgcc
      601 cggagctcgc ctcggtcgtg gcggccgctt tcgcgcaccg gcgcaaggca ctcgcgcgct
      661 cgcttgcgct ggcccccggc gccgcacccg gcatccgcga gaaagcgcgg gaggctctcg
      721 agtcgatggg cctccctgcc gacgctcgcg cggagcggct ctcgccggag cagttcgacg
      781 aactcgagcg gaggctggcg gcatgaccca gcgcgaggtc gcgccggcga aggtgaacct
      841 gctgctgcac gtcggaccgc cgcgggaggc cggcctccac gagctgtgct cgctcttcgc
      901 ctcgatcgac ctcgccgacg agttgggctt cgaggccgca ccggcacttg cggccgacga
      961 ggtcgtctgc cccggtgtgg acgggcgcaa cctgatcgcg gtcgccctgg ccgagttccg
     1021 cgagcgggtc gactcggccc tgccgccgtt gcgcgtggtg gtcgagaagc gcatcccggt
     1081 cgcggccggc ctggcgggcg gcagcgcgga cgccgcggcg gcgcttcgag cggcgaacgc
     1141 gatcgcgggc gagccggcga ccaccgacga gctccgcgcg ctcgccgccc ggctcggctc
     1201 cgacgtgccg agccaggtcg agccgcgcca cgcgctggtc atgggcgccg gcgagcaggt
     1261 cgagccgctg gagctgcccg ggctcgcgct cgtgctcgtc ccctacgccg acgggctgtc
     1321 agccgccgag gtgtacgccg aggctgatcg cctcggctcc acgcgcgagc ggctcgatcc
     1381 cgaaggcgcg cgggcgctcg cgcggctgcc ggcgcaggag ctcgcggcac ggctcgagtc
     1441 cgatctcgag cccgcagtgc tgtcgctgcg ccccggcgtg cgcgacaacc tcgatgcgct
     1501 gctcgaggcc ggcgcgcggg ccgcgctcgt gagcggctcg gggcccaccg cgttcggcgt
     1561 cttcccaaac gacgaggaag cacggcgcgc tgccggcctg atcgacggcg ccattcaggc
     1621 gagactgcgc actgcatgag caccaagcac gtgatcgagc tggtgatcgc cggcgtgctg
     1681 ctggtcgcgg cgctgtggct gcgccacaag cggcggctct ccggcgagcg cttcgtggtc
     1741 gtgcttgtgg tcgcggcgat cctcgcagtg atcggcagcg gggtgctcag cggcttcaac
     1801 gccgagcacg caatcaccga cctcgcgcac gcgctcggca agtggaccta cgcgctcgtc
     1861 ggcgtgctcg cgttcgccga gaccggcgcg ttcatcggat tcatcgcgcc cggcgagttc
     1921 acggtgatcc tcggcggcgt catcgccggc gaggggacga tcaacatctt tctgctgatc
     1981 accctcgtct gggtctgcgc cttcctcggc gacactgtga gcttcttcat tgggcgccgc
     2041 ctcggccgcg agttcctgct gaagcacggc gagcgcttca aggtcacgca cgagcgcctc
     2101 gagcaggtgg agcgcttctt ccaccgccac ggcggcgcga cgatcctgat cggcaggttc
     2161 gtcggcttcg tgcgcccgct cgcgccgttc atcgccggct cgtctcgtct gccgtatcgg
     2221 cgcttcctgc cctacagcgt gctcggcacc gggctgtggg ggtccacctt gtgcgtgctc
     2281 ggctacatct tctaccgctc gttctcgaag atcacctcga tcgccggccg cgcgacgctg
     2341 atcttcagca tcctcgtcgg gctgatcgcc gccgtggtct ggatctccaa gcgcctgcgc
     2401 gatcccgagc aacgccgtgc gctcgagcga tgggccgaca accacgcggt ggtgcgcccg
     2461 ctgtggcacc acgtgatccg tccggcctac cgcgtcctcg ccccgcaggt ccgtttcgtc
     2521 tggcgccggc tcacccccgg cggcctcggc atcgagttca cgagcgcgct cgctgtgagc
     2581 gtcgtaggcc tctacctgtt cacgatgtat ctcctcgcga tcacgagcga tccgggacca
     2641 acggccgcgg acgagttctt ccaggacctc gcgagcgaca ctcgcaccca cgcgctcgtg
     2701 cagctggcaa aggtgatctc cgtgttcggc tctttgcccg tgaccggcgg gctcgtcctc
     2761 gcgggcgcac tggcgctcgc ctggcggcgc cgcccggtgg agatgctggc gctcctctcc
     2821 ggcgcgctca cgatctggat cggcgtgcag gccaccaagg ccggcgtgga ccgtccccgc
     2881 ccggagcccc aactcgtgaa cacgacgggc agcgcctacc cgagcggcca tgccgcctac
     2941 tcaacggtgt atatcgcgct ggcggtgatc ggatggcgag tcctgccggg cctcgcgacg
     3001 aagacgttcg tgatcctcat cgcggcgctc atctccgtgg ctatcggact gtcccgcatc
     3061 tacctgaacg tgcattggtg gtccgacgtc gtcggcggct gggggctcgg ctgtggaata
     3121 ttcggcctga gcgcgtgcgt cgcgctcgtc gtccatcatc tgcgcaacac taggagcgcg
     3181 gaaccgctcc ccgaaccttc ctgagcagtg ggaagcatct cctccaccac actcgttctg
     3241 atcgcggcag gcgcggtcgg cctcgtggcc tacttcacgc tcgtgctcgc gccggcctgg
     3301 tcgtcgtacg ggcgcctgtg ggagcggctg gctgccagct tcctgagcct ctacatcctg
     3361 gcgaccttca tcggggtcgg gctgggaatc ggcttcgcgg tcgtctggtt ctacgaccgc
     3421 tacgccggct gagcccgcaa cccgtccgta cactttgctc tgaatgagca cggtcgaggg
     3481 cggcaaaacc cctgcaaaca gcgggttcat ggactccttc tcggatgtct ccgaggcggt
     3541 tgagtccggc gccggcctgc ccgaggtcgc gcgcgccgcg ggccgcgctc tcggcgccag
     3601 cgtcgcgatc gtcgactcct cgagctccgt cgttgcggtc gcctgtgcgt cgccggcgga
     3661 tgagcgcgct gtgctcgctc gggaggcggg cgtcgaggtg ctcgacctgc gcgtggcgga
     3721 atcgcgcgtc ggcgagctcc gctaccggct gcgcggcgag acgccgccgg cgccgctcct
     3781 gcggatggtg ggcacgctga tcgcgctcga ggtggagcgc tcgcgcgggc ccgagagggc
     3841 gagcgaggcg gccgtcggcg cgttcctcgc ggacctgctc gaccgccgcg ccacggaccg
     3901 cgagaacatc ctcgcccgcg ccaccgagct gggggccgac ctggcgcagg gtggcagcgt
     3961 cgtcgtcgcg cgcgctcacc cgctgcggcc ggccgagggc gactggcgcg cgcgggcgca
     4021 gtccgtcgcc gagcgcggcg cacgtggagt ggcccgcgac acgctcgccg cgacggtcgc
     4081 gctggaagcc gcggcccagt cggtcccgaa cgggccgggc gagctggtgc tggtcgtgcc
     4141 gggcgcggac gccgacctct cacgccgcgt cgcgaccgcc gtgctgcgcg agctggaggg
     4201 cgggatgccg ggcttcgcca tcaccgtggc gcgatcgcgt ccggcgggcg acccggtcga
     4261 tctgcaccga gccggcgcag aagccgtcct cgcggccaac gtggcggagg cgcgcggcgc
     4321 ccaggagctc gccttcgagg agacgggctc ctaccgcctg ctgctgccgg cgatgatcga
     4381 tgaccccgcc gagttacgcc gctttcacga ggagacgatc gcgccactcg tgggctacga
     4441 cgagcagtac gagacggagc tcgtgcgcac gctggagacc ttcctcgacg ccgacggaaa
     4501 cgtcggccag acggccgagc gcctcttcac ccaccgccac acaatccgct accggctcga
     4561 gcgcgtgaag gagctcacag gccttgacgt gagctcgacg gacgggcggg agcggctcgg
     4621 cctcggcctc aaggccatgc gagtgctcgg catcatgccg acgcgcgggc cggccgacga
     4681 ggcgggcacc gaggcgggcc aagtgccccg cagcgaagcc gacatctagg tcgagcgcga
     4741 cttccgtcgc gcaagtgcgt tagaactccc gacgcaatcc tgctgaaccg cccacgccgc
     4801 tccggcggtg ggggcggccg ggggacccaa gttgttgggg cgaatcgccg caaggcgtag
     4861 cgcgactctt tccgcgcgag cccgtcagct aaccccggaa gcgtgacgaa agagaggaag
     4921 tttgatgaag cacgcccgtc tggggatgac gacggctgca tgcatgatgg ccgcgctcgc
     4981 tgcgctggca cccaccgcct cggcaggggt ccgcttcggc cagcgcaccc tgcacatggg
     5041 ttcgcgcgga cacgatgtga aggttcttca gtcgtggctt tcccatctcg ggatctccac
     5101 ctcgatcgac ggtgtgttcg gccccggtac gcgctccaag ctgcaccgct tcgagaaggc
     5161 caacgccggc ttcactgcgg acggccgcct cacccgcagc gaggcgcagc ggctgcgcaa
     5221 gctgatcgag gcgaagtttg gcgcccccac gccggtcgcg ccgccggcca gcaaggcgca
     5281 actgtcctcg gacggccgta cggcggtggc gcccgccgac gcgccgcagc ccgtgaagga
     5341 cgcggtcgcg gccgccaatc agatcacgcg caagccgtac cgctacggcg gcggccacgg
     5401 cagcttcgag gactccggct acgactgctc gggcgcggtg agctacgcgc tccacggcgc
     5461 cggcctgctt agggcgccgc tcgactcgag cggcttctac agctggggca agagcggccg
     5521 cggctcgtgg atcaccgtgt acacgaagtc gagccatgcc tacgtggtga tagccgggct
     5581 gcgcttcgac acctcaggga gcggcgagtc cggtccgcgc tggcgaaccg aggcgcgctc
     5641 gagcgcgggc tacgcggcgc gtcatccgag cggcttatga gctggggagt gagttagggg
     5701 cgaagcagcg ggcgccttcg ggcgcccgct tgctttctgg tgccgaggct gggggggagg
     5761 gactcgaacc cccgctgccg ggaccaaaac ccggtgtcct accagctaga cgacccccca
     5821 gcggcaaagt tgaggatacg gccacacgcg gccgcgctac attgcgcgcg tggcctcgcc
     5881 caccgtcctc atcatggccg cgggccacgg gacccggatg cactcttccg ttccgaaggt
     5941 gctccacccc atctgcggcc gtccaatgct gcattgggtg atcgagtccg cccgcgcggc
     6001 cggggcgggc aagatcgtcg cgatcgtgcg tccgggcgag ggcgtggccg acgcgctgcc
     6061 cgatggcgtg gtcgccgccg agcagacgga tggcgagggc acaggctgcg cggtgctcgc
     6121 tagtcgcgac gcggtcggcg gcagcggcac ggtggtcgta ctgagcggcg accatccgct
     6181 cgtctccgct gagctcatcc gcgagttcgt cgtagcgcac gagcgcgagg gcgccgcggc
     6241 cacgatgctc acctccgagg agctcgaccc gaccggatac ggccgcgtga tccgggcaaa
     6301 cgacggctcc gtcgagcgga tcgtcgagac caagcatccc gaagggctga gcgacgagga
     6361 gctcgccacg cgcgagatca acatgggcgc ctacgccttc gatgccgagg agctgttcag
     6421 cgcgctcgac gaggcgggcg agcagtacgg cgagcgttac ctcaccgacg tctttccgat
     6481 cctgcgcgag cgcggccggc gcatcgccgc ccaccgcaca tccgacgtct cgagcgcgat
     6541 gggagtgaac acgcgcgccg acctcatggc ggtcgccgag cgcctgcagc ggcgcctgct
     6601 cgacgagcac gcaagagcag gtgtgacgtt cgagctcccg ggcagcgtct acctcgaggc
     6661 aggcgtggag atcggacccg acaccgtcat cgcacccggc gtgacgctgc gcggcacgac
     6721 ccggatcggt ggggggtcga cgatcgggcc caacacgacc gtgatcgact ccgagatcgg
     6781 cgacggcgtc acggcgctgc acgcctacct gctcgaggcc caggtcgaga acggcgtctc
     6841 gatcgggccc ttcgcgtacc tgcgccccgg caccgtggtg cgcgaaggcg cgaagatcgg
     6901 gacctacgtg gaggtgaaga actccaacat cgggcgcggc acgaaggtgc cccacctctc
     6961 ttacctcggg gacgccgacg tgggtgagga aacgaacgtc gcggcgggca acatcaccgc
     7021 caactaccac gctggcaaga agacccggac gcgcatcggc gaccacgtgc acacaggggt
     7081 ccacacgtcg ctcgtggcgc cggtgactgt cggggacgac gcgtacactg cagctgggtc
     7141 cgtgatcacg gaagacgtac ctgaaggggc cctcgggatc tccaggcccc cgcagcgaaa
     7201 cgtcgagggc tacgccgaga gaatccggaa ggagaccaag gaatgagcac cgtcgaggtc
     7261 ggcacgcggg cgcgtccaac gaccgtcccg ttcgactacg acaagcggat gatggtcacg
     7321 gccggccgct ccaaccgcga gctggcggcc aagatcgcgg acaagctggg cgtggcgctc
     7381 accgacccgg gcctgaagac cttctccgac ggcgaggtct actgccgcta ccaggagtcg
     7441 atccgcggag cggacctctt catcatccag tccacctgcg ggaacgagcg cgaggggctc
     7501 acggcgaacg acgcgctgat ggagctgctc gtgatggtcg acgccgctgt gggcgcgtcg
     7561 gcgcaccgtg tgatcgcggt cactccgtgg tacggctact cgcgccagga caagaagtcg
     7621 gcgccgcgcg agccgatcac ggcgcgcctc gtggcgaaga tgctcgagac ggccgggatc
     7681 gaccgcctgc tcagcctcga ccttcacgcc ggccagctgc agggcttctt ccaaaagccg
     7741 gtcgaccacc tgaccgcgat gccggtcctc acgcagtacg tgaaggacca gctagcgccc
     7801 gacggcgacc tcgtgatcat cgcgccggac gccggccggg tgaagctcac gcgcaagttc
     7861 gcgcagaagg tgggcgcgcc ctacgcgctg ctcgagaagg agcggccggc gcagcaggtg
     7921 gcggagatcg gctacgtgat cggcgacgtg aaggacaagt gcgccgtgat cgtcgacgac
     7981 atcatcgaca ccggcggcac gctcgccgcg gcggcgcaga cggtgatgga cgagggcgcg
     8041 aagcaggtct acgcgctcgc cacccacggc atcttctccg gcaatgcctt cgagacgctg
     8101 gagcagtcgc cgatcgcggg catcgtggtc accgacacgg ttccgctgcg cgacggcgcc
     8161 ccgcccctca tccgtcagct gagctgcgcc gacatcgtca cggactccgt ccgcgcgatc
     8221 ttcaccgacg actccgtgtc ggagatcttc gcgggcgaga accagctgtt ctgacccccg
     8281 tgtggggctg gtagccgcca gctgacggcg ggtacgactc tggtgtgctg gactccgtcg
     8341 cacacctggt cgaggggaac gtctggggct acttcgtgat cctcgccgtg gtcgccgtcg
     8401 acgcggtctt cccgctgctc ccgagcgaga ccgtgatcat cgccggcgcg gtgctcgcat
     8461 cgaacggcga catgtcgctg acgctgatca tcctcgccac cgcggcgggg gcgatggtgg
     8521 gcgacaacgt ctgcttcttc ctcggggaca aggtcggcga gccgctcgca aggcggctgt
     8581 tcacgtcgcc gaaatcccgg caggtgtacg cgtgggggtc gcgcatgatc tgcgaccaca
     8641 cctggatcgt ctcggtcgcg cgcttcgtgc cgtgcggccg caccgcgacg accttcggtg
     8701 ccggcgccct ggtcgtgagc tggcgctcct tcatccgctg ggacgcgatc ggcgcagtcg
     8761 tctgggccac ctacgcgacg atggtcggct atctcggtgg cgaggcgttc cagaactcgc
     8821 tctggaagcc atttctgctg tcgacagcaa tcgcgctggc cgtcggcttc gccggcgagc
     8881 ttgcgaggcg ccggtctgcg cgacgtctgg cgcgcgaggg caagctccac gtgtcgaacg
     8941 agcggcggca gcgcgctcga gagcagctga gctagcgggc gaaaaccgag ctatgcaagg
     9001 acgcagggcc gaagcttggc gagagccaag cgagtgcccg aggacgccgc agagcgaagg
     9061 tttgcagccc gctagtggcg ctcgctgaag cggcggcgcc gtttctcgcc gcggccctcg
     9121 aagtaggccc actcctcgcg tccgagctcg gtggcgggct cgtcgccgaa ccaccactcg
     9181 cggccgatcc ggttccagtt gcgggtggcg ttcagctgac cgaggacagc gagtagaccg
     9241 gtctccatgc gccgccccat catctcgttg gcgggcaggt tctcgcggcg catcagctgg
     9301 aagaactccg agcgcgggtc ggacgccgcc gagatcgcgc gcatgaccat ctccgagtcg
     9361 acggtcacat cgcggtcctg catgtaccag ccgccgatgg cgcggacctg ctccatgagc
     9421 cggatcgcgt cgactcgctt ggggctgcgg aagaagccga ggtccgcgag cgcctggcgc
     9481 agtcgctcgg cgtcgtcgga gagcacggcc tcgatagccg cgatctcgag ctcgatctga
     9541 tcgtcgtcga gagtcttcgt catgccgaag tcgaggaacg cgacccggtc gtcgtccatg
     9601 agcaggtagt tgccggggtg tacgtcggcg ttgaactgct gcaggtggta gatcgacccg
     9661 aagcagaagc ggaagacgat ctcgccgaag cggtcgcgct cgggctgggg tcgcagcttc
     9721 acctcctcga acgagacgcc gtcgacccac tcggtgacca gaacgcggct gcgcgagagg
     9781 cgcgtcacca cgtcgggcac gtagatgaac gggtggccgc ggtaaccccg cgcgaacgca
     9841 cggtggttct gagcctcgaa ctcgtagtcg agctcctcga gcacacgctc gcgcagctcg
     9901 cgcgccgcgg ccttcgcgtc gaggccgggc gcgatcgcct tcgcgaagcg caggaacacg
     9961 ccggcgttgt ccacgtcggc tctcagcgcg tcggccacct tcgggtactg gatcttcacc
    10021 gccacgcgcc ggccgtccgg cagcaccgcg cggtggacct gcccgatcga ggctgcggcc
    10081 gccgcctcgt ggtcgaagtc ctcgaagagc gactcgacag gctcgtccca ctcctcgtcg
    10141 agcacctgcc gcaccttctt ccacggcata ggcggggccg agct
//
LOCUS       DASVXS010000247        24291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1412803, whole genome shotgun sequence.
ACCESSION   DASVXS010000247 DASVXS010000000
VERSION     DASVXS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24291
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412803"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /gene="rplM"
                     /locus_tag="VF752_13975"
     CDS             <1..266
                     /gene="rplM"
                     /locus_tag="VF752_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX8856700.1"
                     /translation="NAEKISVTGNKRQEKLYYRHSGYPGGLKSRSLEEMLERRPEEVI
                     RLAVKGMLPRTRLGRAQLRKLKIYAGPDHPHAAQQPQPLEVDA"
     gene            266..1315
                     /gene="rpsI"
                     /locus_tag="VF752_13980"
     CDS             266..1315
                     /gene="rpsI"
                     /locus_tag="VF752_13980"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX8856701.1"
                     /translation="MQDEERQDSPQEEQGSEQPAEEQPTAPAEQPEPAAEEQPPEAAE
                     EQPQAGAEEAPEAEEAEAAGEGGSGLGAGAAAEGEELPGEPAEPGAPDIAETIKPDAS
                     DVAEERARETGEALEEAAETAGRPRGKQTERGPRGVKEPKDDVIPGEHLEPIRLEQEL
                     EGARPGDEEFVDTYADQYSGEGGPDAEGATATAEPEVPAPPAEGETAEAAPAPRTPVE
                     LAADARYIATGKRKTSVARVILKPGDGSYLINGRQLDEFFPRVTLQRTARAPLEAAGY
                     EARMDVVARIHGGGVSAQADALRHGVARALVEADPNLRGELKRRGFLTRDARAKERKK
                     AGLKKARKRPQFSKR"
     gene            1324..2682
                     /gene="glmM"
                     /locus_tag="VF752_13985"
     CDS             1324..2682
                     /gene="glmM"
                     /locus_tag="VF752_13985"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX8856702.1"
                     /translation="MGVATPATPPRRLFGTDGVRGVAGDFLSAELALALGRAATADAA
                     GEVPQVLVIRDTRESGEMLESAFAAGVAASGGHALLGGVLPTPAAPLLMRRYAFDLAA
                     VISASHNPYRDNGIKIFGPDCMKLNDEREAAIEAAIHAAEGEEPVPAPQRGIGRIRKL
                     HGADADYLRELEARFQGLRLDGLNVLLDCAHGATYHVAPEIFRRLGATVETVGTEPDG
                     RNINEGVGSTHIEGLAAAMTEGEFDIGFAFDGDGDRVLAVDRNGRVVDGDELIALTAV
                     HLRDTGRLPGNGVAVTLMTNLGFHHAMQAVGIEVAQTPVGDRHVIAELVKRGWALGGE
                     QSGHIIDTGFVPSGDGIAAALLTLEALRGRDLADRSVMTKLPQTLLNVKVRDRSAIEG
                     ATTVWQAVEREQRELGEGGRVLIRPSGTEPLVRVMVEAPDQFQCQSIAGRIADLIERE
                     LR"
     gene            complement(2690..2965)
                     /locus_tag="VF752_13990"
     CDS             complement(2690..2965)
                     /locus_tag="VF752_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856703.1"
                     /translation="MTHEEREFPGDRSDDPEPHDALNNPADEPDETEWPDPYEKREDP
                     ADPPDPDGFGGEEPHRPVGSESTSEPHPSQDPEAPDWEGPKRDKLDE"
     gene            3065..3751
                     /locus_tag="VF752_13995"
     CDS             3065..3751
                     /locus_tag="VF752_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX8856704.1"
                     /translation="MAVEVRREHFSSAVEDYAKAIYYLEEGGGGAVSNNALAERLSVT
                     PASASNMVKKLDGLGLVEHVPYRGVRLTRSGRRVALEILRHHRLLELYLAENLGVPWD
                     RVHDEAEVLEHVLSEELEELIARKLGNPTHDPHGDPIPTRELELEEDHTQSLQALDPG
                     SRGRFVRISDSDPEMLRYLGERGITLGDDFEVVEKQPFDGPVSVRFGRDVHVLGGELS
                     RAMRAEVEAV"
     gene            3748..5031
                     /locus_tag="VF752_14000"
     CDS             3748..5031
                     /locus_tag="VF752_14000"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX8856705.1"
                     /translation="MNKPLETDAIAVPLGGPTPLESIRARGRVRGVLPLLGPAFVAAV
                     AYVDPGNFATNITGGAAHGYMLLWVILGANLMAMLIQTLSAKIGVVTGKNLPEVCREH
                     FAKPVSLSLWVQAELIAMATDLAEFIGAAIALNLLFDVPLFTAGLMTGVVAFAILALQ
                     NRGYRRFELAITALLGLVLLGFLYQTLKIGPNAHDAVKGFIPKFDGSDSVLLATGILG
                     ATVMPHVIYLHSALTQNRIPVRDDREKRKVMRFLRLDVVIAMGIAGVINMSMLIVAAA
                     VFHSGHSDIDTIEGAYNGFDTLVGGGAALAFAVALLSSGFASSSVGTYAGQVVMQGFI
                     GRKIPLLVRRLITMLPALLILAIGLDPTRSLVISQVVLSFGIPFALIPLVIFTSRREV
                     MGSFVNKRVTTVAASIVAGLIICLNVFLLVQTFSG"
     gene            5185..7053
                     /gene="glmS"
                     /locus_tag="VF752_14005"
     CDS             5185..7053
                     /gene="glmS"
                     /locus_tag="VF752_14005"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX8856706.1"
                     /translation="MCGIVGYVGERSCRELLLHGLEKLEYRGYDSAGVSVLVDDHIQS
                     VRAVGNLSHLREAVAEQASEQLAGAGGVVVAETKPTTGIGHTRWATHGRVSEANAHPH
                     FDTQDRIHIVVNGIVENHVELRQRLAAEGAEFTSDTDAEVVAHLIAHLYDGDLVEAVR
                     SAYAQLRGHYAFVAMAADEPELLVGARKECPLVVGVGEGENFIASAIPAFLNETRKVK
                     LVENDEIVVVRRSGVEILSPGGEPITRETTEVDWDETTAEKGGFETFMLKEIHEQPDA
                     VAETVTDRLAGDQVDLGDIGVSDDELRDLRRITVVACGTSYHAGLVGRYAIEEWARVP
                     VEMDIASEYRYRNPVVGPGDLVIGISQSGETADTLAAMRLARERGAKVLAITNVMGSQ
                     ATRDSDGVLFTRAGIEVGVAATKTFVSQVAAMYLLGLRLAELRGTLPEERLAELRAEL
                     KHLPTLVGETLEKCDQRVREIAEHWSASNFFLYLGRHAGLPICLEGALKLKEISYIPT
                     DAYAAGEMKHGPIALLDERTPVVTVCTDSPVLEKVLSNVAEVQARGAHVIAVASETSP
                     HVAEHAEQVLFVPKADWLIQPMLAVLPLQLLAYYIARARGLNVDQPRNLAKTVTVE"
     gene            7065..7418
                     /gene="acpS"
                     /locus_tag="VF752_14010"
     CDS             7065..7418
                     /gene="acpS"
                     /locus_tag="VF752_14010"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX8856707.1"
                     /translation="MAGIGIDLLEIERLERALERRPRMAERLFSEREREYAAAQARPG
                     QHLAARFCAKEAVAKALGLERWAFRDVEVIATGGAPSVRLSGSAAERARELGVEPVVS
                     LTHTRFTAAAVALLR"
     gene            7415..8956
                     /locus_tag="VF752_14015"
     CDS             7415..8956
                     /locus_tag="VF752_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX8856708.1"
                     /translation="MTLPDWLDPVYDAAEMGASDAWAIEQQGVPSLDLMERAGAGLAR
                     LTADVAQARPGRIRVVVGKGNNGGDGLVAARLLRAEGLDVDVLAVGDLSDLKGDPLAN
                     LERLPGEPPRPFDAAEIDGSSVIVDAMLGTGFAGAPREPVAGAIAAINESGAPVVACD
                     MPSGVDASSGEVEGDAVRAVATATFHGSKIGLHVEPGKTYAGEVHVIAIGIPRGAPAP
                     ERAGLITPRVIELVPHRLREGSKFDSGTVVVAGGGAGLTGAPTMAAHAAMRTGAGYVQ
                     LAVPAVAEMVFEIKLTESMTHALPDHDGAHTEEGAAQVAELAERAGAVVVGPGIGRSD
                     GAFAFARSVAREVGAPLVIDADALNAHAERLEALRERRAPTVLTPHAGELGRLLGRDS
                     AEIAAHRIDSAREAAERSGAIVVLKGDDSLVAHPGGPVAISPGASPALATAGTGDVLS
                     GVIATLFAKRVEPFAAVCAGVLVHAHAGIEAAARLGADHVVAGDAIESIPAAFAGLRT
                     EAGKS"
     gene            8959..10101
                     /gene="alr"
                     /locus_tag="VF752_14020"
     CDS             8959..10101
                     /gene="alr"
                     /locus_tag="VF752_14020"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8856709.1"
                     /translation="MARHAPERALARVDLGAVERNCAQLQSLLGGRARLCAVVKADGY
                     GHGANWVAKAALAGGADWLAVATADEAAELRHHGVDSRILVMGALTPEDARTAIEAVA
                     DVVVWREGFVPVLAERLPDGAAPARVHVKLDTGMGRLGATTVDEALAVADLVAAEPRL
                     ELAGAMTHFATADEDGDDFFGAQLERFNEFAATARSRHPGILVHAANSAATFRDPAAH
                     FDMVRCGIAVYGLDPFQGDPAERGLEPALSLTSYVARVQRFEPGETAGYGRRWTAESP
                     TLVATLPIGYGDGWRRNLSNNCEVLIRGRRYPLVGTVSMDNITVELGPDSDVEVGHEA
                     VLIGRQGGERIRAEEVAQRLGTINYEVTCGLTQRVKRHWHARDEDA"
     gene            10098..11516
                     /locus_tag="VF752_14025"
     CDS             10098..11516
                     /locus_tag="VF752_14025"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8856710.1"
                     /translation="MTLESQQPVREVRAALEDAPERIWIVGGTIRDDLLGRSVTDVDL
                     AVEGDPQAVARRVADAAGGALFALSDEFGSWRSIAPDRSWLCDVSPLQGATIEDDLAH
                     RDFTVNAMAVPLAGGEILDPHGGRSDLDAGVLRVLGPQAYEADPLRALRAARLATELP
                     LKLDVETARLTRQAAPRLADVSAERVFAELRRIVIAERVLEGIALAEDLELVPIVLPE
                     LTALHGVEQSHFHHLDVHDHTLEVLRQQVWLEDALGEVFPEDAERVQAALDEPLANEL
                     TRGQALRFAALLHDVGKPATRGELPDGRVSFIGHDRVGAEMVRQIFRRLRASERLSQF
                     VAANTRHHLVLGFLVHERPLSRRAVYRYLTTTEPVEVEVTLLSCADRLATRGKNAEPA
                     IKAHLDLAREVMHDALAWREGGPPELPLRGDELAAEVGIAPGPELGELLALLREARFA
                     GEAGTREEAVELARSLRQNSAT"
     gene            11513..12481
                     /gene="corA"
                     /locus_tag="VF752_14030"
     CDS             11513..12481
                     /gene="corA"
                     /locus_tag="VF752_14030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX8856711.1"
                     /translation="MIVDTAVYENGRRREGEVPLEKAYDSALEPGAFVWIGLAHPTAR
                     EFDAVAREFNLHELAVEDAVKAHQRPKIEQYDDVVLIVLKSARYDDAAETIEIGELLV
                     FAGESFVISVRHGEGSPLSDVRERLEHNEELLRCGPAAVVHAIVDKVVDDYSPIADSL
                     EEDIDQVEQEVFSEDRTNPAERIYKLKREVLKFHRATAPLMTALEGVVGGAIEIPEPL
                     SEYFRDVLDHAQRVNDRVEAFDDLLTSALDANLTQVSVRQNDDMRKISAWVAIAAVPT
                     VVGAIYGMNFEHMPELKWELGYPAALLVMLVIAALLYRQFRKAGWL"
     gene            12493..12900
                     /locus_tag="VF752_14035"
     CDS             12493..12900
                     /locus_tag="VF752_14035"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX8856712.1"
                     /translation="MPDDCIFCKILEGELPSARIYEDEHTIAFLDINPWTRGHSLVVP
                     RNHSRDLLDASDEDLAHVIAASKRVAGLLRDKLSADGINLLNSCGEAAWQTVFHLHMH
                     VIPRYVDDPLRLPGRPQQTDPEELAELAEEIRA"
     gene            12893..13666
                     /locus_tag="VF752_14040"
     CDS             12893..13666
                     /locus_tag="VF752_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8856713.1"
                     /translation="MPSARFERAGDVGVVVIDQPPLNLFGEEMFRDLGAAVAQAEDSD
                     IRGLLIRAEGEYFTGGVDVHVFDDKTPEQMTEAFEEWVGLVQRLEDMPIPTMSSVQGL
                     CLTAGLELSLGCDLIFASESARFGLVEAVVGLTPAMGGTQRMAERAGPARAREFVMSG
                     DLYPAATLEDWNVVNRVVPGHRLEHESLEFMQRLAAGPTKAHAATKAMVRAFLEHGAR
                     GADERIGTIASPLFDTADLKRAVKMFLAEGPGKAKFDGT"
     gene            13748..13951
                     /locus_tag="VF752_14045"
     CDS             13748..13951
                     /locus_tag="VF752_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8856714.1"
                     /translation="MPTGTVKWFNDEKGFGFITPDEGDNDLFVHHTGIDAQGFRTLEQ
                     NAKVSYEEEQGDKGPKAVNVRPA"
     gene            13986..14204
                     /gene="infA"
                     /locus_tag="VF752_14050"
     CDS             13986..14204
                     /gene="infA"
                     /locus_tag="VF752_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX8856715.1"
                     /translation="MAKEEKIEMEGEVVEALPNTMFRVKLDNEHEVLGHISGKMRRHY
                     IRILPGDRVKIELSPYDLDRGRITYRYK"
     gene            14378..15073
                     /locus_tag="VF752_14055"
     CDS             14378..15073
                     /locus_tag="VF752_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856716.1"
                     /translation="MRVFVFLVTFKRLVPVIAALGVLAVAAPASASVSEVGGRLPKVP
                     ASCPDACQAIGKVSGFQAQVGTVKHPYTMRRAGKIVAFSITLGSPKADQIDFFTKLFG
                     GKPQARLIILRQPRHSKKYLETGMSDLFDLEDYFGSTPTFALKQPLTVRPGYIVAISV
                     PTWAPAFSGTLGNDQIWRSSRDPAKCDDVQQDAAQDVLGSQRTYGCLYRKARLLYTAT
                     FVPDPKKTTPQKN"
     gene            15175..15528
                     /locus_tag="VF752_14060"
     CDS             15175..15528
                     /locus_tag="VF752_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856717.1"
                     /translation="MTAEGWIFMVGFRVFDVGLLVLWLVWFFRLREDEDSDDDGGNGG
                     GGPQPERPTGPGGGGLSLPLGRFSPGARRRDGHNPTHGPRRGRGGEPVMPSVVPRIRR
                     PELPVPVHRRGATTN"
     gene            15621..16358
                     /locus_tag="VF752_14065"
     CDS             15621..16358
                     /locus_tag="VF752_14065"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX8856718.1"
                     /translation="MAPRAPQIVAFGGGGFSMERGNTLLDDYVLSLADSERPRICFLP
                     TASGDADHYIVRFYRAFSPSRCEPSHVSLFRREGGASDMHEHLLTRDVIYVGGGSVTS
                     LIGAWCAHGLDVTLRRAWERGVILCGLSAGSLCWFEEGVSAFHGPPRHVRGLGLLPWS
                     NCVHFDGEPAREQVYRELLADGMRSGYAAEDGAALHFIGTRLSKVVSSRASARAYRMR
                     RVGARVERRALTAEYLGSLPAALVAAA"
     gene            16358..17185
                     /locus_tag="VF752_14070"
     CDS             16358..17185
                     /locus_tag="VF752_14070"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX8856719.1"
                     /translation="MGRNWKRGERRLIFAMGGGGFSSSHGDPALDRYVLEVAEAASPR
                     ICLLPTASGDPQEQIERFYRAYGELACEPDHVSLFRLGANPVDLRTLLLGQDVIYVGG
                     GSMLNLLAIWRAHGLDAILREAWERGVLLCGISAGSMCWFSAGVTNSRGAPRAVRGLG
                     FLPPSNSVHYASQPDRRPCFHEAVRCEAIPPGYGVDDGVGLLFAGTELVEAVSARPDA
                     GAWWVEESRGALVQTALEPLLLSAPEPESTPLSIAEFRALRRRMPAGGNRLAVRLED"
     gene            complement(17200..17592)
                     /locus_tag="VF752_14075"
     CDS             complement(17200..17592)
                     /locus_tag="VF752_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856720.1"
                     /translation="MRERTALALALLALGVAGCGGGGGEKSAETVPAAPVLTSPGEPT
                     PTVEDTHTDTTPTETTPAGTGGTSPGGSTGNSGSQGTPSTPSPQNGGTSAPNGGANAP
                     GTDNSSPNTPGSPVQKFDKFCKENPGAC"
     gene            complement(17604..17873)
                     /locus_tag="VF752_14080"
     CDS             complement(17604..17873)
                     /locus_tag="VF752_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856721.1"
                     /translation="MGERQKYRLRARNDREVIREIEPGSVYVDRETGEEFEVVAALTP
                     LAPSRSELPWAAENLRLCGCSLEQLVPKDLNDCLYCGRRLPATEG"
     gene            17948..19525
                     /locus_tag="VF752_14085"
     CDS             17948..19525
                     /locus_tag="VF752_14085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8856722.1"
                     /translation="MPLVRVLVFHGYLLRGTGSNIYNASLAAALVKLGHEVHILCQDR
                     HASELDFVDAVGTFENGSLSLETLREPVRCTAYLPEIGRILPVYVADEYEGFEAKPFG
                     ELTGAELDHYLDANVAAVRTVAEAVKPEVALANHAVMGPAILAQALGDEVPYAVKIHG
                     SALEYTVRPDPERFLPYAREGIEPAKGVLVGSRHMAERLWELVDVESLREKTRLGPPG
                     VDTDAFTPLPRREAAARVKELSAKLRRGGARWGGDANGWAALRALDPEHEPVVAYVGK
                     LIVSKGVDLLIAAWPLVVERVPNARLCVVGFGTYRGALTRLIDALANGDLAAAREIAA
                     RGRELEGGPTGELKLLASFLDSLEGERRERYLGLAAYGAERIHFTGRLEHEDLPLLLP
                     ACEAQVVPSTFPEAFGMVVVEGAACGLLPIAADHSGLAEVTDELELVLPDELRSLMSF
                     RLEQGSVEAIAERLLTWLELDPKERRHAGCALADRAAERYGWDHVASGVVAAAQGRLD
                     ALPSTEPAGSPSLRPSG"
     gene            19548..20267
                     /locus_tag="VF752_14090"
     CDS             19548..20267
                     /locus_tag="VF752_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8856723.1"
                     /translation="MGTRGRLAALAVAPIVIVLAVGCGSRDSKPDLVNGKTLFVGKGT
                     CGSCHALARANTKGTQGPNLDDAFGTDRRDGIGEDTIQGVIEGQIAHPRRGSIMPAGL
                     VKGDNARDVAAYVASVAGVPGKDSGALAKVGQQQGAGKTAVEKSGKLTIPADPSGALA
                     FEFGKATAKPGKVTFEMPNQSPVGHDIAIKGDGASGKGPVVNKGGTSTFSTTLKPGTY
                     EFYCSVPGHEQAGMKGTLTVK"
     gene            20541..22325
                     /locus_tag="VF752_14095"
     CDS             20541..22325
                     /locus_tag="VF752_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX8856724.1"
                     /translation="MSPENPTLVEDAPVEAAANGNGNGADPKAWTGITAPSARGRSVR
                     RIGEVIVDLGYAPAEVVEKAVAEARESGQPTGKVLVETGVLTPDQLARAVAERYGLDH
                     LDLRIFRVDMGALNLVSAAAARRYEAIPVAFSDDNTLLVAMADPANVLAVDDIAMMTG
                     YEVRRAVTSREDIDALIAQINRLDTAVEETEDESAEAIDLGDLRESAEDAPIVKLVYS
                     IIADAVTQGASDIHFDPEGKDMRVRFRVDGVVSDSTTVPRSMVAGVVSRIKIMADLNI
                     SETRRPQDGRVSLVVEGRQVDIRVVTLPIVRGESVVMRILDRSNVVMDLESLGMADHE
                     RTAFEASIKRAHGSVLVTGPTGSGKTTTLYAALTAVNTPEKTMITIEDPVEYQLDGIK
                     QVQVNPKVGLSFAAGLRSMLRADPNVIMVGEVRDRETAQIAVEAALTGHLVLSTLHTN
                     DAPAAITRLIEMGIEPFLVASSLDCVVAQRLARTLCECRQPVELSAASLKEAGFNATK
                     KVSSYEPGGCNRCGGTGYKGRIGLYEVMPVTREIRQLVLERRSADEISELAREQGMRT
                     LRDDGLEKVVAGVTSISEVSRVTAGWTD"
     gene            complement(22615..23385)
                     /locus_tag="VF752_14100"
     CDS             complement(22615..23385)
                     /locus_tag="VF752_14100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8856725.1"
                     /translation="MSAPAADLELAERAALVAGRELMARYGEPARGVERKSSDTDMVS
                     EADRAAEAAIVELLSRERPGDGMLGEEGSAAASESGRRWVVDPLDGTTNYLYRFPAWC
                     VSVALEDADGALVGVVHEPYTGHVWRASRGGGCELDGSPVGAREPLSLDRALIATGFG
                     YSPERRRMQAEALRTIIPRVRDIRRPGAAALDLASVACGRLDGYYERGLNPWDWAAGR
                     LLVSEAGGVVRDLPGEPHGLVAAGPSLVDELFELVAGA"
     gene            complement(23501..24118)
                     /locus_tag="VF752_14105"
     CDS             complement(23501..24118)
                     /locus_tag="VF752_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX8856726.1"
                     /translation="MESASVAADHAHEHAHPAPPAANQSARIDAQTLGMYLFIISEIM
                     LFGAFFTAYFFLRVVNHDDWPAAGMDIPVVVAGVNTAILVSSSFTMHWALVSAKNENR
                     FGMKAGLATTFLLGLTFLTVQINEYVHVGFAPADSAQGTIFYGLTGLHGAHVTVGLML
                     LAFATIRAFRGHFSAAEHRGVEVPGIYWHFVDLMWILVYTSLYIL"
BASE COUNT         3721 a         8283 c         8725 g         3562 t
ORIGIN      
        1 tgaacgccga gaagatctcg gtcactggca acaagcgcca ggagaagctg tactaccggc
       61 attccggcta tcccggcggt ctcaagagcc gctcgctcga ggagatgctc gagcgcaggc
      121 cggaagaggt gatccgcctg gccgtgaagg gcatgctgcc ccgcacgcgc ctcggccgcg
      181 cccagctccg caagctgaag atctacgcag gcccggatca tccgcacgcc gcccagcaac
      241 cgcagccttt ggaggtcgac gcctgatgca ggacgaggag cgccaagact ccccgcagga
      301 ggagcagggc tccgagcagc ccgccgagga gcagccgacg gctcccgcgg agcagccaga
      361 gcccgctgcc gaggagcagc cgccggaagc tgctgaggag cagccgcagg ccggtgccga
      421 ggaggctccc gaggcggagg aggcggaggc cgcgggcgag ggcggctcgg ggctcggtgc
      481 cggggcggcc gccgagggtg aggagcttcc gggcgagccc gccgagccgg gagcccctga
      541 catcgccgag accatcaagc ccgacgcatc cgacgtcgcc gaggagcgcg cccgcgagac
      601 cggcgaggcc ctcgaggagg cggcggagac ggcagggcgc ccgcgcggca agcagaccga
      661 gcgcggcccg cgcggcgtga aggagccgaa ggacgacgtg atccctggtg agcacctcga
      721 gccgatccgg ctcgagcagg agctcgaggg cgcgcgcccg ggcgacgagg agttcgtcga
      781 cacctacgcc gaccagtaca gcggcgaggg cgggccggac gccgagggcg cgaccgccac
      841 cgcggagccg gaggtgccgg cgccgccggc ggaaggcgag accgccgagg ccgctcccgc
      901 tccgcgcacc ccagtcgagc tggccgccga cgcccgctac atcgcgaccg gcaagcgcaa
      961 gacctcggtg gcccgcgtga tcctcaagcc gggcgatggc agctacctca tcaacgggcg
     1021 ccagctcgac gagttcttcc cccgcgtgac gctccagcgc accgcccgcg cgccgctcga
     1081 ggccgcaggc tacgaggctc gcatggacgt ggtcgcgagg atccacggcg gcggtgtgtc
     1141 cgcccaagcc gacgcgctac gccacggcgt ggcgcgcgcc ctcgttgagg ccgacccgaa
     1201 ccttcgcggc gagctgaagc gccgcggctt cctgacgcgc gacgcgcgtg ccaaggagcg
     1261 gaagaaggcg ggcctcaaga aggcgcgcaa gcggccgcag ttctcgaagc gctagggccg
     1321 cccatggggg tggccactcc ggcgacgccg ccccggcggc tcttcgggac cgatggcgtc
     1381 cgcggagtcg ccggcgactt cctgtctgcg gagctggcgc tcgcgttggg gcgcgcggcg
     1441 acggcggacg ccgccggcga ggtgccgcag gtgctagtga tccgcgacac gcgcgaatcc
     1501 ggcgagatgc tcgagtcggc gttcgcggcc ggtgtcgccg cgtccggcgg ccacgcattg
     1561 ctcggcggtg tgctgcccac acccgccgcg ccgctgctga tgcgtcgcta cgcgttcgac
     1621 ctcgctgccg tgatctcggc ttcccacaac ccgtaccgcg acaacgggat caagatcttc
     1681 ggccccgact gcatgaagct caatgacgag cgcgaggccg ccatcgaggc cgccatccat
     1741 gcggccgagg gggaagagcc ggtgcccgcg ccgcaacgcg gcatcggccg catccgcaag
     1801 cttcacggcg ccgatgccga ctacctgcgt gagctcgagg cccgcttcca gggcctgcga
     1861 ctcgacgggc tgaacgtgct gctcgactgc gcccacggcg ccacctacca cgtggccccc
     1921 gagatcttcc gccgccttgg cgcgacggtc gagacggtcg ggaccgagcc cgacggccgc
     1981 aacatcaacg agggcgtcgg ctcgacgcac atcgaggggc tagcggcggc catgacggag
     2041 ggcgagttcg acatcggctt cgccttcgac ggcgatggcg atcgcgtgct cgcggttgac
     2101 cgcaacggcc gggtcgtcga cggcgatgag ctgatcgcgc tcaccgccgt gcacctacgc
     2161 gacacgggcc ggctgccggg caacggcgtc gctgtgaccc tgatgacgaa tctcggcttc
     2221 caccatgcga tgcaagccgt cggcatcgag gtggcgcaga ccccggtggg ggaccgccac
     2281 gtgatcgccg agctcgtgaa gcgcgggtgg gcgctcggcg gagagcagtc aggtcacatc
     2341 atcgacaccg gcttcgtccc atcgggtgac ggcatcgccg ccgcactcct cacgctggag
     2401 gcgctccgcg gacgcgacct cgccgaccgc agcgtgatga cgaagctgcc gcagacgctc
     2461 ttgaacgtga aggtgcgcga tcgcagtgcg atcgagggcg cgacgaccgt atggcaggcg
     2521 gtcgagcgcg agcagcggga gctcggcgag ggcgggcgcg tgctcatcag gccctcgggc
     2581 acggagcctc tcgtgcgcgt catggtggag gcgccggatc agttccagtg ccagtcgatc
     2641 gcgggccgca tcgcggacct gatcgagcgc gagctgcgct aactcgcgct cactcgtcca
     2701 gcttgtcgcg cttcgggccc tcccagtcgg gcgcctcggg gtcctgcgac ggatgcggct
     2761 cgctcgtcga ctccgagccg accgggcggt ggggctcctc gccgccgaaa ccgtccggat
     2821 ccggtggatc ggcgggatcc tcccgcttct cgtatggatc cggccactcg gtctcgtccg
     2881 gctcgtcggc cgggttgttc agcgcgtcgt ggggctccgg gtcgtcggag cggtcgccgg
     2941 ggaactctcg ctcttcgtgg gtcatgtcga gcgagtaccc aacggagcct gcgggtaatc
     3001 gacgccgtcc atttgtccac ggacggcata tttcagattt agtcatgcca taatcaaggt
     3061 cgatatggcg gttgaagtgc gacgcgaaca tttctcctcg gcagttgagg actacgcgaa
     3121 ggcgatctac tacctggagg agggcggcgg tggggccgtg tccaacaacg ccctcgccga
     3181 gcgcctgtcg gtcacgcccg cgtcggcctc gaacatggtc aagaagctcg acgggctcgg
     3241 cctcgtcgag cacgtgccgt accggggcgt gcgtctcacc aggtcagggc gccgtgtggc
     3301 gctcgagatc ctgcgacacc accggctgct cgagctgtat ctcgcggaga acctcggcgt
     3361 gccgtgggac cgagtgcacg acgaggccga ggtgctcgag cacgtgctct ccgaggagct
     3421 cgaggagctg atcgcgcgga agctcggcaa cccaacgcac gacccgcacg gcgacccgat
     3481 ccccacgcgc gagctcgagc tagaggagga ccacactcag agtctgcagg cgctcgaccc
     3541 cggctcgcgc ggccgctttg tccgcatttc ggacagcgac cccgagatgc tccgctacct
     3601 cggcgagcgc gggatcacgc tcggcgacga cttcgaggtc gtcgagaagc agccgttcga
     3661 cggaccggtg tccgtgcgct tcgggcgcga cgtgcacgtg ctcggcggcg aactgtcgcg
     3721 cgcgatgcgc gccgaggtgg aggcggtatg aacaagccgc tcgagaccga cgccattgcc
     3781 gtaccgcttg gcggtccgac tccactcgag agcatccggg cccgcgggcg cgtgcgcggc
     3841 gtgctgccgc tgctcggacc ggcgttcgta gcggccgtcg cgtacgtcga cccgggcaac
     3901 ttcgccacga acatcaccgg cggggccgcg cacggctaca tgctgctgtg ggtcatcctc
     3961 ggcgccaacc tcatggcgat gctgatccag acgctttcgg cgaagatcgg cgtggtcacc
     4021 ggcaagaacc tccccgaggt ctgccgcgag cacttcgcca agccggtctc gctgagccta
     4081 tgggtgcagg ctgagctgat tgccatggcc accgacctcg cagagttcat cggcgccgcc
     4141 atcgcactga acttgctctt cgacgtgccg ctgttcaccg ccggcctgat gaccggcgtg
     4201 gtggcgttcg ccatcctcgc gctgcagaac cgcggctacc gccgcttcga gctggcgatc
     4261 accgccctgc tggggctcgt gttgctgggc ttcctctacc agacgctcaa gatcgggccg
     4321 aacgcgcacg atgcggtcaa ggggttcatc ccgaagttcg acggctcgga cagcgtgctg
     4381 ctcgccaccg gcatcctcgg cgcgacagtt atgccgcacg tgatctacct gcactcggcg
     4441 ctcacgcaga accgcatccc ggtgcgcgac gaccgcgaga agcggaaggt gatgcgcttc
     4501 ctgcgcctcg acgtggtgat cgccatgggc atcgccggcg tgatcaacat gtcgatgctg
     4561 atcgtggccg ccgccgtctt ccattccggc cacagcgaca tcgacacgat cgagggcgcc
     4621 tacaacggct tcgacacgct cgtcggcggc ggcgcggcgc tcgcgttcgc ggtcgccctg
     4681 ctgtcctcgg gcttcgccag ctctagcgtg ggcacctacg cgggccaagt ggtcatgcag
     4741 ggcttcatcg gccgcaagat ccccctcctc gtccgtcgcc tgatcacgat gcttcctgcg
     4801 ctgctgatcc tcgccatcgg gctggatccc acccgctcgc tcgtcatcag ccaggtcgtc
     4861 ctgtccttcg gaatcccgtt tgcgctgatc ccactcgtga tcttcacgag ccgccgcgag
     4921 gtgatgggca gcttcgtgaa caagcgcgtt acgactgtcg cggcctcgat agtcgcgggc
     4981 ctcatcatct gcctcaacgt cttcctgctg gtgcagacct tctctggctg attccgctct
     5041 gggagcgggt ttcgaaccct gggcgggccg cggtgccact ggtaccatcg agatgccctc
     5101 cgggagggct cttcgagccg catgagcgcg gctttcgtcg ttcttagagg gttcgaccct
     5161 cgtccacgcg aaagggccag ggacatgtgc ggaatcgtgg gatacgtggg ggagcggagc
     5221 tgccgagagc tcctgctgca cggcctcgag aagctcgagt accgcggtta cgactcggcc
     5281 ggcgtctccg tgctcgtgga cgaccacatc cagtcggttc gcgccgtggg caacctctcg
     5341 cacctgcgtg aggcggtagc ggaacaggcg agcgagcagc tggcgggcgc aggcggcgtc
     5401 gttgtcgccg agaccaagcc gacgaccggt atcggccaca cccgctgggc cacccacggc
     5461 cgggtgagcg aggcgaacgc gcacccgcat ttcgacacgc aggaccgcat ccacatcgtg
     5521 gtcaacggca tcgtcgagaa ccacgtagag cttcgccagc gcctcgccgc cgagggcgcg
     5581 gagttcacct ctgacaccga cgccgaggtg gtggcgcacc tgatcgccca cctctacgac
     5641 ggcgacctgg tcgaggccgt tcggtcggcg tacgcgcagc ttcgcggcca ctacgcgttc
     5701 gtagcgatgg cggccgacga gcccgagctg ctcgtgggcg cccgcaagga gtgcccgctc
     5761 gtggtgggag tgggcgaggg cgagaacttc atcgcctcgg cgatccccgc gttcctcaac
     5821 gagacccgca aggtgaagct cgtcgagaac gacgagatcg tcgtcgtgcg ccggagcggc
     5881 gtggagatcc tcagcccggg cggcgagccg atcacgcgcg agacgaccga ggtcgactgg
     5941 gacgagacga ccgccgagaa gggcggcttc gagaccttca tgctcaagga gatccacgag
     6001 cagcccgacg ccgtcgcgga gacggtcacc gaccgcctcg ccggcgacca ggtggacctc
     6061 ggcgacatcg gcgtctcgga cgacgagctc cgcgacctcc gccgcatcac ggtcgtcgcg
     6121 tgcggcacct cgtaccacgc cgggctcgtc ggccgctacg cgatcgagga gtgggcgcgc
     6181 gtgccggtcg agatggacat cgcctccgag taccgatatc gcaacccggt ggtgggcccg
     6241 ggggacctcg tgatcggcat ctcgcagtcg ggcgagaccg ccgacacgct cgcggccatg
     6301 cggctcgcgc gcgagcgcgg cgccaaggtg ctcgccatca cgaacgtgat gggcagccag
     6361 gccacccgcg actccgacgg tgtgctgttc acgcgcgccg gcatcgaggt cggcgtggcg
     6421 gccacgaaga ccttcgtgtc gcaggtggcg gcgatgtacc tgctcgggct gcgcctggcg
     6481 gagctgcggg gcacgctgcc ggaggagcgg ctcgccgagc tgcgcgctga gctgaagcac
     6541 ctgccgacgc tcgtgggcga gacgctcgag aagtgcgacc agcgcgtgcg cgagatcgcg
     6601 gagcactggt cggcctcgaa cttcttcctc tacctcggcc gccacgccgg cctgccgatc
     6661 tgcctcgagg gcgccctgaa gctgaaggag atctcgtaca tccccaccga cgcgtacgcg
     6721 gcgggagaga tgaagcacgg cccgatcgcg ctgctcgacg agcgcacgcc ggtcgtgacc
     6781 gtgtgcaccg actcgcccgt gctcgagaag gtgctctcca acgtggccga ggtgcaggcg
     6841 cgcggcgcgc acgtaattgc ggtcgcgagc gagacctcgc cgcatgtggc ggagcacgcg
     6901 gagcaggtcc tgttcgtgcc aaaggcggac tggctgatcc agccgatgct ggcggttctg
     6961 ccgctgcagc tgctcgccta ctacatcgcg cgggcgcgcg ggctgaacgt ggaccagccg
     7021 cgcaacctcg cgaagacggt caccgtcgag tagcttcccg gcgcgtggcc gggatcggta
     7081 tcgacctgct cgagatcgag cggctcgagc gagcgctcga gcgcaggcct cgcatggccg
     7141 agcggctctt cagcgagcgg gagcgcgaat acgcagcggc acaggcgcgg cccggccagc
     7201 atctcgccgc gcgcttctgc gcgaaggagg cggtcgcgaa ggcgctcggc ctcgagaggt
     7261 gggcgttccg ggacgtcgag gtaatcgcca ccggcggcgc tccgagcgtg cggttgagcg
     7321 ggagcgccgc tgagcgcgcg cgggagctcg gcgtggagcc ggtcgtctcg ctcacacaca
     7381 cgcgcttcac ggccgcggcg gtggcgctgc tgcgatgacc ctgccggact ggctcgaccc
     7441 cgtctacgac gctgccgaga tgggcgcgtc ggatgcctgg gcgatcgagc aacagggcgt
     7501 gccctcgctc gacctgatgg agcgcgctgg tgcggggctc gcgcggctga ccgccgacgt
     7561 ggcgcaagcg cgcccgggcc ggatccgggt ggtggtaggc aagggcaaca acggcggcga
     7621 cggcctcgtc gccgcgagac tgctgcgagc ggaggggctc gatgtcgacg tgctcgcggt
     7681 gggcgatctg agcgacctga agggcgatcc gctcgccaac ctcgagcgac tgccgggcga
     7741 gccgccgcgg ccgttcgatg ccgccgagat cgacggctcc tccgtgatcg tcgacgcgat
     7801 gctcgggacg gggttcgcgg gagcgccgcg cgagccggtg gcgggagcga tcgccgcgat
     7861 caatgagtcg ggcgcgccgg ttgtggcgtg cgacatgccg tcgggcgtgg atgcttcgag
     7921 cggcgaggtc gagggcgacg cggtgcgcgc ggtcgcgacg gccaccttcc acggctcgaa
     7981 gattggcctt cacgtcgagc ccggcaagac gtacgcgggc gaagtgcacg tgatcgcgat
     8041 cggcatcccg cgcggcgcgc ccgcccctga gcgtgccggc ctcatcacac cgcgcgtgat
     8101 cgagctcgtg ccgcaccggc tgcgtgaggg ctcgaagttc gactcgggga ccgtggtggt
     8161 cgcgggcggc ggcgccggcc tcaccggagc gccgacgatg gcggcccacg cggccatgcg
     8221 caccggcgcc ggctacgtgc agctcgccgt gcccgcagtt gccgagatgg tgttcgagat
     8281 caagctgacg gagtcgatga ctcacgcgct gcccgaccac gacggagcgc acacagagga
     8341 gggtgcggct caggtggccg agctggcgga gcgtgccggg gcggtcgtgg tggggccggg
     8401 gatcgggcgc agcgacggag cgtttgcgtt cgcccgctct gttgctcgcg aggtcggggc
     8461 gccgctggtg atcgacgcag acgccctcaa tgcgcacgct gagcggctcg aggcgttacg
     8521 cgagcgccgc gctccaaccg ttctgacgcc gcacgccggc gagctcggcc gcctcctcgg
     8581 tcgcgatagc gcggagatcg ccgcccaccg catcgactct gcgcgtgagg ccgcggagcg
     8641 gagcggcgcg atcgtggtgt tgaagggcga cgattcgctg gtcgcacatc cgggcgggcc
     8701 ggttgcgata agccccggtg caagcccggc gctcgcgacc gccggtacgg gcgatgtgct
     8761 ctccggtgtg atcgcgacgc tcttcgcaaa gcgggtcgag ccgtttgcag cggtctgcgc
     8821 gggagtgctg gtgcacgcgc acgcgggcat cgaggccgcc gctcggctcg gagccgacca
     8881 cgtggtcgcg ggcgacgcga tcgagtcgat tccggctgcg ttcgcagggc tgcgtaccga
     8941 ggcggggaag tcctagacat ggcgcgccac gccccggagc gggcgcttgc ccgcgtggac
     9001 ctcggcgccg tggagcgaaa ctgcgcgcag ctacagtcgc tgctcggcgg gcgcgcgcgg
     9061 ctgtgcgcgg tcgtgaaggc cgacggctac gggcacggag cgaattgggt tgccaaggcg
     9121 gcgctcgccg gcggcgccga ctggctcgcg gtggccacgg ccgacgaggc cgccgagctc
     9181 cgccaccacg gtgtcgactc acgcatcctg gtgatgggtg cgctcacgcc cgaggacgcg
     9241 cgcaccgcga tcgaggcggt ggccgacgtg gtggtgtggc gtgagggctt cgtgccggtg
     9301 ctggccgagc gcctcccgga cggcgcggcg ccggctcgcg tgcacgtgaa gctcgacacc
     9361 ggcatggggc ggctcggcgc gacgacggtc gacgaggcgc tcgctgtggc cgacctcgtg
     9421 gccgcggagc cccggctcga gctcgcgggc gcgatgacgc acttcgcgac cgccgacgag
     9481 gatggcgacg acttcttcgg cgcgcagctc gaacgcttca acgagtttgc cgcgacggcg
     9541 cgcagccgcc accccggcat cctcgtgcac gcggccaaca gcgccgcaac gttccgcgac
     9601 cccgccgcgc acttcgacat ggtgcgctgt ggcatcgccg tctacggcct cgacccgttc
     9661 cagggcgacc ccgccgagcg cgggctcgag ccggcgctct cgcttacctc ctacgtggcg
     9721 cgagtgcagc gcttcgagcc gggcgagacg gcgggctacg gccggcgctg gacagccgag
     9781 tcgccaacgc tcgtcgcgac tctgccgatc gggtacggcg acggatggcg gcgcaacctc
     9841 tcaaacaact gcgaggtgct gatccgcggg cggcgctacc cgctcgtggg cacggtgagc
     9901 atggacaaca tcaccgtcga gctcggcccc gacagcgatg tggaggtcgg gcacgaggcc
     9961 gtgctgatcg gccggcaggg cggcgagcgc atccgcgcgg aggaggtcgc gcagcggctc
    10021 ggcacgatca actacgaggt cacctgcggg ctcacgcagc gcgtgaagcg ccactggcac
    10081 gcgagggacg aggacgcgtg acgctcgagt cgcagcagcc ggtgcgggag gtccgcgccg
    10141 ctctcgagga cgccccggag cggatctgga tcgtgggcgg cacgattcgc gacgacctgc
    10201 tcggccgctc ggttaccgac gtcgacctcg ccgtggaggg cgatcctcag gcggtagcgc
    10261 gcagggtggc cgacgcggcg ggcggagcgc tgttcgcgct gtcggacgag ttcggctcct
    10321 ggcgctcgat cgcgccggac cgctcgtggc tatgcgatgt ctcgccgctc cagggcgcga
    10381 cgatcgagga cgacctcgcc catcgggact tcaccgtcaa cgcgatggcc gttccgctgg
    10441 ccggcggcga gatcctcgac ccgcacggcg ggcgctccga tctcgacgcc ggagtgctgc
    10501 gcgtgctcgg gccgcaggcg tacgaggcgg atccgctgcg ggcgctcagg gcggcgcgct
    10561 tggcgaccga gctgccgctg aagctcgacg tggaaacggc tcggctaacg cgccaggcgg
    10621 cgccgcggct ggctgatgtc tctgcggagc gcgtgttcgc cgagcttcgc cgcatcgtga
    10681 tcgccgagcg cgtgctcgag ggcatcgcgc tcgccgagga cctcgagctg gtgccgatcg
    10741 tgctgcctga gctcacggca ctgcacggcg ttgagcagag ccatttccac cacctggacg
    10801 ttcacgatca cacgctcgag gtgctgcgcc agcaggtctg gctcgaggac gcgcttggcg
    10861 aggtgttccc ggaggacgcc gaacgggttc aggccgcact cgacgagccg ctcgcgaacg
    10921 agctcacgcg cggccaggcg ttgcgcttcg ccgcgctgct gcacgacgtc gggaagccgg
    10981 ccacgcgcgg cgagctgccg gacggccgcg tgagcttcat cgggcacgat cgcgtcggcg
    11041 ccgagatggt ccggcagata ttccggcgcc tgcgcgctag cgagcggctc agccagttcg
    11101 tggccgcgaa cacgcgccac catctggtgc tcggcttcct cgtgcacgag cgcccgctgt
    11161 cgcggcgcgc cgtctaccgc tacctcacca cgaccgagcc agtcgaggtg gaggtgacgc
    11221 tgctctcatg cgcggaccgc ctcgccacgc gcggcaagaa cgcggagccg gcgatcaaag
    11281 cgcatctcga cctggcgcgt gaggtgatgc acgacgcgct cgcctggcgc gagggcggac
    11341 cgccggagct gccgctgcgc ggggacgagc tcgcggcgga ggtcgggatc gcgcccggac
    11401 ctgagttggg agagctgctc gcgctcctgc gagaggcgcg cttcgccggc gaggcgggca
    11461 cgagagagga agctgtcgag ctcgcccgct cgctgcgcca gaattccgcc acatgatcgt
    11521 cgacaccgct gtttacgaga acggccgtcg gcgcgagggc gaggtgccgc tcgagaaggc
    11581 gtacgactcg gcgctcgagc cgggagcgtt cgtatggatc gggctcgcgc acccgacggc
    11641 ccgggagttc gacgccgtcg cgcgcgagtt caacctgcac gagctggccg tcgaggacgc
    11701 ggtgaaggcg caccagcgcc ccaagatcga gcagtacgac gacgtcgtac tgatcgtgct
    11761 gaagagcgcc cgttacgacg acgcggcgga gacgatcgag atcggcgagc tcctcgtgtt
    11821 cgccggcgag tcgttcgtga tctcggtgcg ccacggcgag ggcagtccgc tgtccgacgt
    11881 acgggagcgg ctcgagcaca atgaggagct cttgcgctgc ggacctgccg cggtggtgca
    11941 cgcgatcgtg gacaaagtgg tggacgacta cagcccgatc gcggactccc tcgaggagga
    12001 catcgaccag gtcgaacagg aggtgttctc ggaggaccgg acgaaccccg ccgagcgcat
    12061 ctacaagctc aagcgcgagg tgctgaagtt ccaccgtgcc acggccccgc tgatgacagc
    12121 gctcgagggc gtagttggcg gagcgatcga gatcccggag ccgctcagcg agtacttccg
    12181 cgacgtgctc gaccacgctc aacgcgtgaa cgaccgggtc gaggccttcg acgatctgct
    12241 cacgagcgca ctcgacgcga acctcacgca ggtgagcgtt cgccagaacg acgacatgcg
    12301 caagatctcg gcctgggtgg cgatcgccgc cgttccgacc gtcgtcggcg cgatctacgg
    12361 aatgaacttc gagcacatgc ccgagctcaa atgggagctc ggctatccgg cggcgctctt
    12421 ggtgatgctc gtcatcgccg cgctgctgta ccggcagttc aggaaggccg gctggctgta
    12481 ggctgcccgg ccatgcctga cgactgcatc ttctgcaaga tcctggaggg tgagctgccg
    12541 agcgcccgca tctacgagga cgagcacacg atcgcgttcc tcgacatcaa cccgtggacg
    12601 cgcgggcact cgctcgtggt gccgcgcaac cactcgcgcg acctgctcga cgcaagcgac
    12661 gaggacctgg cgcacgtgat cgcggcatcc aagcgggtgg ccggcctcct gcgggacaag
    12721 ctcagcgctg acgggatcaa cctcctcaac tcctgcggcg aggccgcgtg gcagacggtg
    12781 tttcacctgc acatgcacgt gatcccgcgc tacgtcgacg atccgctcag gctgccgggc
    12841 cggccccagc agacggatcc agaggagctc gcggagctcg ccgaggaaat ccgtgcctag
    12901 cgcgcgcttc gagcgcgcgg gcgacgtggg cgtggtcgtg atcgaccagc cgccgctcaa
    12961 cctgttcggc gaggagatgt tccgtgacct cggcgctgcg gtggcgcagg ccgaggactc
    13021 agacatccgc ggccttctga tcagggccga gggcgagtac ttcaccggcg gggtggacgt
    13081 ccacgtgttc gacgacaaga cacccgagca gatgaccgag gcgttcgagg agtgggtggg
    13141 cctggtccag cggctcgagg acatgcccat ccccacgatg agctcagtgc aggggctctg
    13201 cctgaccgcc ggcctcgagc tgtcgctcgg atgtgacctg atcttcgcct cggagtcggc
    13261 gcgcttcggg ctcgtggagg cggtggtcgg cctcacgccc gcgatgggag gcacgcaacg
    13321 catggcggag cgcgccggac ccgcccgtgc acgcgagttc gtgatgagcg gcgatctcta
    13381 cccagcggcg acgctcgagg actggaacgt ggtgaatcgc gtcgtgccgg gccaccggct
    13441 cgagcacgag tcgctggagt tcatgcagcg cctcgcggcc gggccgacga aggcccacgc
    13501 cgcgacgaag gcgatggtgc gggcgttcct cgagcacggt gcgcgcggcg cggacgagcg
    13561 catcggcacg atcgcgtcgc cgctgttcga caccgcggac ctcaagcggg ccgtgaagat
    13621 gttcctcgcc gagggacccg gcaaggcgaa attcgacggc acctgaccga cgccaaaacg
    13681 ctcgcgaaat cgcgggggtc ggtgatacgt tcgcaccgta gggcgatcta tcgaagaggg
    13741 gctaggcatg ccgacgggta cggtcaagtg gttcaacgac gaaaagggct tcgggttcat
    13801 cacacccgac gagggcgaca acgacctgtt cgtccaccac accggaatcg acgctcaggg
    13861 cttccgtacg ctcgagcaga acgcgaaggt ctcctacgag gaggagcagg gcgacaaggg
    13921 cccgaaggct gtgaacgtcc ggccggcatg acctgccgcc agcccgcctt ctagactgag
    13981 gtcccttggc gaaggaagaa aagatcgaga tggagggcga ggtcgtcgag gccttgccca
    14041 acacgatgtt ccgagtgaag ctcgacaacg agcacgaggt gctcgggcac atctcgggga
    14101 agatgcggcg tcactacatc cgcatcctcc ccggggaccg ggtcaagatc gagctcagcc
    14161 cgtacgacct agaccgcggt cggatcacgt atcgatacaa gtagctccgc tcgatctcga
    14221 ctcgagcgcg ggcgctgcaa agcgtcggct ggctgcgtca tcggcagact cgtgtgcaga
    14281 gcacacttcg cttgccatcc tcgtttttcg cgcccgttcc cgggctcgat ttactagggt
    14341 cctggccgca cgtcgcgaat accgcaccaa tcctgcggtg cgcgtgttcg tttttctcgt
    14401 gactttcaag cgactcgttc ccgttattgc tgctctcggc gtgctcgccg tggccgcgcc
    14461 cgcgtccgcg tcggtgtcgg aggtcggtgg gcggctgccc aaggttccgg cgtcgtgccc
    14521 ggacgcgtgt caggcgatcg ggaaggtgag cgggttccag gctcaggtcg ggaccgtgaa
    14581 gcacccgtac acgatgcggc gcgccggcaa gatcgtcgcg ttctcgatca cgctcgggag
    14641 cccgaaggcc gaccagatcg acttcttcac caagctcttc ggcggcaagc cgcaggcgcg
    14701 cctgatcatc ctgcgccagc cgcggcactc gaagaagtac ctcgagaccg gcatgagcga
    14761 cctgttcgat ctcgaggact acttcggctc gacgcccacc ttcgcgctca agcagccgct
    14821 gaccgtgagg ccgggctaca tcgtggcgat ctcggtgccc acgtgggcgc cggcgttctc
    14881 gggcacactc gggaacgacc aaatctggcg ctcgtcgcgc gatcccgcca aatgcgacga
    14941 cgtgcagcag gacgccgccc aggacgtgct cgggagccag cgcacctacg ggtgcctgta
    15001 ccgcaaggcg cgtctgctct atacggccac gttcgtgccg gatccgaaga agaccacccc
    15061 gcagaagaac tagcgcctga ctgctggcgc ctacgcgcca gccgcctgag ggctgggaat
    15121 ttcacccggg acgggggatt tgcgccgcat cggcagagcg gtactatcgc gatgatgacc
    15181 gccgaggggt ggatcttcat ggtgggcttc cgggtgttcg acgtcgggct gctcgtgctc
    15241 tggctcgtgt ggttcttccg cctccgggag gacgaggaca gcgacgacga cggcggtaac
    15301 ggcggtggcg gaccgcagcc cgagcgcccc accggccccg gcggcggggg tctctcactc
    15361 ccgctcggcc gcttctcgcc gggcgctcga aggcgcgacg gtcataaccc cacgcacggc
    15421 ccgcgccgcg gtcgtggggg tgagccggtc atgccgagcg tcgtgcctcg gatcaggcga
    15481 ccggagctgc ctgtgcccgt tcaccgccgc ggcgctacga cgaactgagc gcgcgattcg
    15541 acccggtggc gaaccgggcc gggtgaattt ccgacacgcg tcgatgtcac gccgcgcggc
    15601 ttcccacgag cattgctgtc atggcaccgc gggcgccgca gatcgtcgcg ttcggcggcg
    15661 gggggttctc gatggagcgc ggcaataccc tgctcgacga ttacgtgctc tcgctcgcgg
    15721 attcggagcg gccgcggatc tgctttctgc cgaccgcgag cggtgacgcg gaccactaca
    15781 tagtccgctt ctaccgggcc ttctccccat cgcgctgcga gccgtcgcac gtttcgctgt
    15841 tccggcgcga gggaggcgcc tcggacatgc atgagcatct gctcacgcgc gacgtgatct
    15901 acgtgggagg cgggagcgtc acgagcttga tcggcgcgtg gtgcgcacat ggcctcgacg
    15961 tgacgctccg ccgcgcgtgg gagcgcgggg tgatcctgtg cgggctgagc gcagggtcgc
    16021 tgtgctggtt cgaggagggc gtgagcgcgt tccacgggcc gccccggcac gtgcgcggcc
    16081 tcggcctgct gccctggagc aactgcgtgc acttcgacgg cgagccggcg cgtgaacagg
    16141 tctaccgcga gctcctcgcg gacggcatgc gctccggcta cgccgctgag gacggcgcgg
    16201 cgctccactt cattggcacg cgcctctcga aagtggtgag ctcgcgcgcg tccgcccgcg
    16261 cctaccgcat gcgacgtgtc ggcgcgcgcg tcgagcgtcg agcgcttacc gcggagtacc
    16321 tcggctcgct cccggccgct cttgtggcgg ccgcctgatg gggcggaact ggaagcgcgg
    16381 cgagcgcagg ctcatctttg cgatgggcgg aggcggcttc agcagctctc acggcgaccc
    16441 cgctctcgac cgctacgtgc tcgaggtcgc agaggcggcg tcgccgcgca tctgcctgct
    16501 acccacagcg agcggcgacc cgcaggagca gatcgagcgc ttctaccgcg cctacggcga
    16561 gctcgcgtgc gagccggatc acgtgtcgct cttccggctc ggcgcgaacc cggtcgacct
    16621 ccgcacgctt ctcctcgggc aggacgtgat ctacgtcggc ggcggcagca tgctgaacct
    16681 gctcgcgatc tggcgggcgc acggcctcga cgccatcctg cgcgaggcct gggagcgcgg
    16741 cgtcctgctc tgcggcatca gtgccggctc gatgtgctgg ttcagcgccg gcgtgacgaa
    16801 ctcgcgcggt gccccgcgcg ccgtgcgcgg cctcggtttc cttccgccta gcaactcggt
    16861 ccactacgcc agccagccgg accgccggcc gtgcttccac gaggccgtcc gatgcgaggc
    16921 gatcccgccg ggctatgggg tcgacgacgg cgttggcctg ctgttcgcgg gcacggagct
    16981 cgtcgaggcg gtcagtgccc ggcctgacgc cggtgcctgg tgggtcgagg agtcacgcgg
    17041 tgcgttggtc cagacggcgc tcgagccgtt gctcctgtcg gcgcccgagc cggagtccac
    17101 gccgctctcg atagccgagt tccgagcgct gcggaggcgg atgcctgccg gcggcaaccg
    17161 cctggcggtc cggctcgagg actgactagg ccgccccgcc tagcaggcgc ccggattctc
    17221 cttgcagaac ttgtcgaact tctggacggg gctgcccggg gtgttcggcg agctgttgtc
    17281 ggtcccaggc gcattcgcgc cgccgttcgg ggcggaggtg ccgccgttct gcggggacgg
    17341 cgtcgacggc gtcccctggc tgcccgagtt gccggtgctg ccaccggggc tggttccgcc
    17401 ggtgcccgcg ggcgtggtct cggtgggcgt cgtgtcggtg tgagtgtcct ccaccgtagg
    17461 tgtcggctcg cccggtgacg tcagcacggg ggcggccggg acggtctctg cgctcttctc
    17521 gccacccccg ccgccgcatc cggcaacgcc gagagcgagg agagcgagcg caagtgcggt
    17581 gcgctctcgc atgttgtggt ggctcagcct tcggtcgcgg ggaggcggcg gccgcagtac
    17641 aggcagtcgt tgaggtcctt cggcacgagc tgctccaaag agcagccgca gagcctgagg
    17701 ttctcggctg cccacggcag ctcgctccgg gagggcgcga gcggggtcaa cgccgcgacc
    17761 acctcgaact cctcaccggt ttcgcggtcc acgtagacgc tgccgggctc gatctcccgg
    17821 atcacctcgc ggtcgttccg cgcgcgcagc ctgtacttct ggcgctcgcc catgaggcgg
    17881 gaaacatagc agtgccacct gtcgctccga ggcccgaaac ggtggctcgg gcggcacgtg
    17941 agacgatatg ccgctcgtgc gagtactcgt gttccacggc tacctcctgc gagggacggg
    18001 cagcaacatc tacaacgcga gcctggcggc ggcgctcgtg aagctcggcc acgaggtgca
    18061 catcctctgc caggaccggc acgcgtcgga gctcgatttt gtggacgcgg tcggcacctt
    18121 cgagaacggc tcgctcagtt tggagacgct gcgcgagccg gtccgctgca ccgcctacct
    18181 gcccgagatc gggcggatcc tgccggtcta cgtggccgac gagtacgagg gcttcgaggc
    18241 gaagcccttc ggcgagctca cgggcgctga gctagatcac tacctcgacg ccaacgtggc
    18301 ggcggtgagg acggtggccg aggcggtgaa gccggaagtg gcactcgcca accatgcggt
    18361 gatggggccg gcgatactcg cgcaggcgct cggcgacgag gttccgtacg cggtgaagat
    18421 ccacggcagc gcgctcgagt acaccgtgcg cccggacccc gagcgcttcc ttccgtacgc
    18481 gcgcgagggg atcgagcccg cgaagggcgt gctggtcggc tcccggcaca tggccgagcg
    18541 gctctgggag ctcgtggacg tggagagcct gcgcgagaag acccgccttg gcccgcccgg
    18601 cgtcgacacc gacgcgttca caccgctgcc gcgccgcgag gcggctgcgc gggtgaagga
    18661 gctctcggcg aagctgaggc ggggcggcgc gcgctggggc ggcgacgcga acggctgggc
    18721 ggcgcttcga gcgctcgacc ccgagcacga gccggtcgtg gcgtacgtgg gcaagctgat
    18781 cgtgtcgaag ggcgtcgacc tgctgatcgc cgcctggccg ctcgtggtcg agcgcgtacc
    18841 caacgcccgc ctgtgcgtgg tcggattcgg cacctaccgc ggggcgctca cgcgcctgat
    18901 cgacgcgctc gcgaacggcg acctggcggc ggcgcgcgag atcgcggcgc gcggaaggga
    18961 gctcgagggc ggtccgacgg gcgagctgaa gctgcttgcg agcttcctcg acagcctcga
    19021 gggcgagcgg cgtgagcgct acctcggctt agcggcgtac ggagccgagc gcatccactt
    19081 caccggaagg ctcgagcacg aggacctgcc tctgctgctg ccggcgtgcg aggcccaggt
    19141 ggtgccgagc acattcccgg aggcgttcgg catggtggtg gtcgaggggg cagcatgcgg
    19201 cctgctgccg atcgccgcgg atcactccgg tctcgccgag gtgaccgacg aactcgagct
    19261 cgtgctgccg gatgaactgc gctcgctcat gtcgttcagg ctcgagcagg gatcggtcga
    19321 ggccatcgcc gagcggctcc taacgtggct ggagctcgat ccgaaggagc ggcgccacgc
    19381 gggctgcgcg ctagcggatc gcgcggccga gcgctacggc tgggaccacg tcgcgagcgg
    19441 ggtcgtcgcg gccgcgcagg ggcggctcga cgcccttccc agcacggagc cggccggatc
    19501 gccttcactc cgcccgagcg gataatcttc ccgccgactt gcaccggatg gggacaaggg
    19561 ggaggctcgc ggccctggcc gtcgcgccca tcgtgatcgt ccttgcagtg ggctgcggct
    19621 cgcgggactc gaagccggac ctggtcaacg gcaagacgct gttcgtgggc aagggcacgt
    19681 gcggcagctg ccacgcactc gcccgcgcga acacgaaggg cacacagggc ccgaacctcg
    19741 acgacgcgtt cggcaccgat cggcgtgacg gcatcggcga ggacaccatc cagggcgtga
    19801 tcgagggcca gatcgcccat ccgcgccggg gcagcatcat gcccgccggc ctggtgaagg
    19861 gcgacaacgc acgtgacgtc gccgcctacg tggcgtccgt cgcgggcgtg ccgggcaagg
    19921 actcgggggc gctcgcgaag gtcggccagc agcagggggc cgggaagact gcggtcgaga
    19981 agagcggaaa gctcacgatc cccgccgatc catcgggcgc gctcgccttc gagttcggca
    20041 aggcgaccgc gaagcccggc aaggtcacgt tcgagatgcc gaaccagtcg ccggtcgggc
    20101 acgacatagc catcaaaggc gatggcgcga gcggcaaggg gccggtcgtg aacaagggcg
    20161 ggacgtccac cttcagcacc acgctcaagc ccgggaccta cgagttctac tgctcggtgc
    20221 cggggcacga gcaggcaggc atgaaaggca ctctcacggt gaagtagggc gccgcgcgcc
    20281 gcggctctgc gagcgcttac agcggcggta aaggcgggtg gtgccggggc cgccgccgag
    20341 actgcagcct tttgggcgct atagcaacca aaaggctgca aagttcgctt cggctcgact
    20401 ccctaactgc ctaaccatcc cacccggcgt accacgagcg ccgcgagctg accacgggtc
    20461 gataggcgga aatgacgccg ccgtcatctc cgctcaggat ctctccacta cctgccgata
    20521 gagggggagg cggggatcac gtgagcccgg agaatccgac actcgtcgaa gacgcgcccg
    20581 tcgaggcagc cgcgaacggg aacggcaacg gcgccgaccc gaaggcctgg actggcatca
    20641 ccgcgccgag cgcgcgtggt cgctccgtgc gccggatcgg cgaggtgatc gtggatctcg
    20701 ggtacgcgcc cgccgaggtg gtggagaagg cggtcgccga ggcccgcgag agcggccagc
    20761 cgaccggcaa ggttctcgta gagacgggcg tgctgacgcc cgaccagctc gcccgcgcgg
    20821 tcgcagagcg ctacggcctc gatcacctcg acctgcgcat cttccgcgtg gacatgggcg
    20881 cactcaacct ggtgagcgcg gccgccgccc gccgctacga ggcgatcccg gtggcgttct
    20941 cggacgacaa cacgctgctc gtggcgatgg cggatcccgc gaacgtgctc gcagtcgacg
    21001 acatcgcgat gatgaccggc tacgaggtcc gccgcgcggt cacgtcgcgc gaagacatcg
    21061 acgcgctgat cgcgcagatc aaccgcctcg acaccgcggt ggaggagacc gaggacgaga
    21121 gcgccgaggc gatcgaccta ggcgacctgc gcgagtcggc cgaggacgcg ccgatcgtca
    21181 agctcgtcta ctcgatcatt gccgacgccg ttacgcaggg cgcctccgac atccacttcg
    21241 acccggaggg caaggacatg cgcgtccgct tccgggtgga cggcgtggtc tccgactcga
    21301 ccaccgtgcc gcgcagcatg gtcgcgggcg tggtgagccg catcaagatc atggccgacc
    21361 tcaacatctc ggagactcgc cggccgcagg acggtcgcgt gagcctcgtg gtcgagggcc
    21421 gccaggtgga catccgcgtc gtgacgctgc cgatcgtgcg cggcgagtcc gtcgtgatgc
    21481 gaatcctcga ccgctcgaac gtcgtcatgg acctcgagag cctcggcatg gcggaccacg
    21541 agcgcacggc cttcgaggca tcgatcaagc gcgcgcacgg ttcggtgctc gtgaccggcc
    21601 ccactggctc cggcaagacg accacgctct acgcggcgct caccgccgtg aacacgcccg
    21661 agaagacgat gatcacgatc gaggatccgg tcgagtacca gctcgacggg atcaagcagg
    21721 tgcaggtgaa cccgaaggtc gggctgagct ttgccgccgg actccgctcg atgctccgtg
    21781 ccgacccgaa cgtgatcatg gtcggcgagg tgcgcgaccg cgagaccgcg cagatcgccg
    21841 tcgaggccgc gctcacgggc cacctcgtgc tctccacgct ccacacgaac gacgccccgg
    21901 ctgccatcac tcgcctgatc gagatgggca tcgagccctt cctcgtggcc tcgtcgctcg
    21961 actgtgtcgt ggcccagcgc ctggcccgca cgctgtgcga gtgccggcag ccggtcgagc
    22021 tttcggcggc cagcctgaag gaagctgggt tcaacgccac gaagaaggtc tcctcgtatg
    22081 agcccggtgg atgcaatcgc tgcggcggca ccggctacaa gggccgcatc ggcctctacg
    22141 aggtgatgcc ggtgacacgc gagatccgac agctcgtgct cgagcgccgc tcggccgacg
    22201 agatctcgga gctcgcgcgc gagcagggca tgcgcaccct gcgcgacgac gggctcgaga
    22261 aggtcgtggc gggcgtcacg tcgatctccg aggtctcacg agtgaccgca gggtggacgg
    22321 actgaggagc gtcatgaccc ccgcgcgaca gagatatgcg agtgaggctt tggagctcac
    22381 gcgcgcgaca cttcaccgcg tggaggatcg cctcgaggac tcgggcgtga gtgtcgacgg
    22441 cgtggagctt caaggcgact tccccgacgg cgagatcgtg gtctcctacg actgcgccgg
    22501 cgagcactgc gagtgccact acgcggtctg gggaccgctc agcgagttcg gcgaagagaa
    22561 gccgcgctcg cttgcgctcg agatcaccgc tcaggtcgtg gagctcgccg ccggctacgc
    22621 accggccacc agctcgaaca gctcatcgac gagcgaggga cccgccgcaa ccaagccgtg
    22681 cggctcgccg ggcaggtcgc gcaccacgcc gcccgcctcg ctcaccagca agcgcccggc
    22741 cgcccagtcc caggggttga ggccccgctc gtagtagccg tcgaggcgcc cgcaggcgac
    22801 gcttgcaagg tcgagggccg ccgcacccgg tcggcggatg tcgcgcacgc gcgggatgat
    22861 cgttcgcagc gcttcggctt gcatcctccg ccgctccggc gaatagccga agccggtcgc
    22921 tatcagcgct cggtcgaggc tcaatggctc gcgcgctccg accggcgacc cgtcgagctc
    22981 gcagccgccg ccgcgcgagg cacgccacac gtgtccggtg tagggctcgt gcacgacgcc
    23041 cacgagcgcg ccatccgcat cctcgagcgc cacgctcacg caccaggcgg ggaagcggta
    23101 gaggtagttc gtcgtgccgt cgagcgggtc caccacccag cggcggccgc tctcgctcgc
    23161 ggccgccgac ccctcctcgc cgagcatccc gtcgccgggg cgctcgcgcg aaagcagctc
    23221 gacgatcgca gcctccgcgg cgcggtctgc ctcggagacc atgtcggtgt cgctcgactt
    23281 gcgctcgacg ccgcgagcgg gttcgccgta gcgcgccatc aactccctgc ccgccacgag
    23341 cgccgcgcgc tcggcgagct cgaggtcggc ggccggcgcg ctcagaagat cgctcgcagg
    23401 atcagcacga tcgccacgat cgcgaccacg acggcgacgg cccgcagcag gaagcggaac
    23461 gcttcttctt ccgacctgag cggattcagc acgccgcgcc ctacaggatg tagagcgagg
    23521 tgtacacgag gatccacatc agatcgacga agtgccagta gatgcccggc acctcgacgc
    23581 cccggtgctc cgcggcgctg aagtggccgc ggaaggcgcg gatcgtggcg aacgccaaga
    23641 gcatcaggcc gaccgtgacg tgcgcgccat gcaggccggt gagcccgtag aagatcgtgc
    23701 cctgcgcgct gtcggccggt gcgaagccga cgtggacgta ctcgttgatc tgcaccgtga
    23761 ggaacgtgag gccgagtagg aaggtggtgg ccaggcccgc cttcatgccg aagcggttct
    23821 cgttcttggc cgacacgagc gcccagtgca tcgtgaagct cgaggacacc aggatcgcgg
    23881 tgttcacgcc ggcgaccacg accgggatgt ccatccccgc ggcgggccag tcgtcgtggt
    23941 tcacgaccct caggaagaag taggccgtga agaatgctcc gaagagcatt atctcggaga
    24001 tgatgaagag gtacatcccg agcgtctgcg cgtcgatacg cgcggactgg ttcgcggcgg
    24061 gcggcgcggg atgcgcgtgc tcgtgagcgt gatcggcggc tacggaggcg gattccatca
    24121 ggcggtcacc tcggaggcct ctgcttcgct caccaccacg tgctccacgg ggagcgacgt
    24181 agcggcgcgc acgcgctcga ccaggtcgga gcgaagccag ccggagcgag tggcagggaa
    24241 cgtggagatc acgatctcat cgacggcgta gaactggagg gcgttctgga t
//
LOCUS       DASVXS010000248        11923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_564080, whole genome shotgun sequence.
ACCESSION   DASVXS010000248 DASVXS010000000
VERSION     DASVXS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564080"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF752_14110"
     CDS             complement(<1..204)
                     /locus_tag="VF752_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856727.1"
                     /translation="MEGGATAAMALAAARALYRRTAAARAAVGTRQEPPPPGVVSALE
                     RAVLAAQRAAGEVAAAGRPAAAAD"
     gene            complement(279..1106)
                     /locus_tag="VF752_14115"
     CDS             complement(279..1106)
                     /locus_tag="VF752_14115"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX8856728.1"
                     /translation="MTTMSIGPLVSASWLREHARDSRVRVVDCRFTLTDPAAGERGYL
                     EGHIPGAAFMDLNRHLAGPPGGPEGGRHPLPDPERFAESARRAGISRDSAVIAYDEQG
                     EGGAARLWWLLRHHGHERVAVLDGALGAWTAAGGPLTAGTEVIAPGDFEPSPGPDGDT
                     AEAVEIERRLGDGSLALVDARAAERFRGENEPIDPVAGHIPGARNVPFAELAPGGAMP
                     APDEIHARLPDSSELVAYCGSGVTACVLVLAADVAGRRARLYPGSWSEWSSRGLPAD"
     gene            1105..2103
                     /locus_tag="VF752_14120"
     CDS             1105..2103
                     /locus_tag="VF752_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX8856729.1"
                     /translation="MIAVVRAMVLDNPGTPLHEADLPVPEPGPGELLVRVHACGVCRT
                     DLHIVDGQLPHPKLPLVVGHQIVATVEQAGDGAERHPVGTRVGMPWLWSADGTCPYCR
                     SGRENLCPNARFTGYDVDGGYAEYTVGRDDFCLALPEGYPDLQAAPLLCAGLIGYRSL
                     RMAGDGERLGLYGFGASAHIICQVAVHQGRRVFAFVREGDEAAASLALELGAEWAGDS
                     MKPGPEELDAAIIFAPAGPLVPAALRATARGGIVVCAGIHMSDIPSFPYELLWGERVL
                     RSVANLTRQDGVEFLELAPRVPVRTTVNTYELERANDALDTLRAGEIRGAAVLTLS"
     gene            complement(2106..2894)
                     /locus_tag="VF752_14125"
     CDS             complement(2106..2894)
                     /locus_tag="VF752_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856730.1"
                     /translation="MRTPSLLRQPPIWREALVGLELAQLTRHPIFRGEGVKPAGGQPV
                     MLIPGFLAGDDSLGVMTHWLRRTGHHTKSAGMRSNIDCSEATVRRLSERVECLADKQG
                     QRVALIGQSRGGTIARVLAVRRPDLVSGIVTLGSPVSNSFAIHPLVTVPILAVGLLGT
                     LGARGLFKHSCRNGRCCECFWEDLATPFPEEVGYVSVYSKRDGIVDWQACLEECAEHV
                     EIETSHCGMAVSPTAYRTIAESLARFRRDDARTRKPVQPLQRAA"
     gene            3008..3952
                     /locus_tag="VF752_14130"
     CDS             3008..3952
                     /locus_tag="VF752_14130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX8856731.1"
                     /translation="MRAATIRDEQIVLEDHPDPSPGKGEVLVEVHAAGVNGADLLQRK
                     GLYRAPPGSHQDIPGLELAGTVAALGPGATRFKEGDRVMAVVGGGGQAERAVVHERQL
                     MPVPESLSWAEAGGVPEVFTTAHDMLFTQLGLGPGERLLVHGAAGGVGTAAVQLGRAT
                     GARVTATVRNESLRDAVEELGAGGVIPPEVFGEAGPFDVILELVGAPNMPGNLTALEN
                     RGRIGVIGIGAGAKAELNLGLVLGKRATVCGSTLRPRPLEEKADAARRMERQVLPLFD
                     TGALRVPVADTFPLDRAAEAYERFEAGGKLGKIVIVTR"
     gene            complement(3942..6380)
                     /locus_tag="VF752_14135"
     CDS             complement(3942..6380)
                     /locus_tag="VF752_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8856732.1"
                     /translation="MRTSGQSLRSQVLIPLAVAIALVALCVGLACTLSARNAAKDGVA
                     ARAEAARATFEQAARPSRRLPGPASVRRALAEAAASGAGVAFGEKGANATLAPQQGGH
                     ETFTYRLTNPPGTLTVSVPSDAIGKATTSALVKTLGIGLGAFLVFFPLIASLLDRRLA
                     APLRSLADAVAKGLAGDTEARAAVEGPAEIVAVTGRVNDLLQAQVESTANAEKAENTD
                     YLTGAASNRQFQETLGIEIKRAQREQNSVGLVVLGLDRFESINDNYGHAKGDEVLRNV
                     AEELRDMLRATDVLSRLGGDEFALILPGTDPDVAYSIAERARSVIAELSPNGRRLTSS
                     AGVACYPDHARDESTLLQFANGALRLAKREGKNQSRRYDPEVVVVPRGEDELAEVEAL
                     FELEEPIVPFFQPLVALSTGKILGFEALARFPHPPQRGPDAWFAQAHRCNLGAKLEAM
                     AIEAALEHQNRPAGTFLSINVSPTIIATPEIQEVLPEDMSNLVVEITEHELADDIDTV
                     LAELQKLRDRGARIAVDDAGSGYSGLQQVMLVHPDVIKLDRSLVMGLNDDPAKVALID
                     SFVRFARRTGATVCAEGIETMEELRTLADLDVTYGQGYALARPADDWTSVSSAVSEAL
                     LRRSLQSQADVTADSDPTDSGDKRLEFVSARISQVGSLDELGELLELIAAEVGSDDVT
                     VSRWLPEDDSVETICGGDQAPAGERFKLSNYPSTRHVLTTQEAVQVLVSDPGADLGEV
                     ALLQRTGFAALLMVPVVCRGETLGLIEALSRTERPWTRSEINRARIISYQLGSAIDGI
                     ARESGAEQAAAATA"
     gene            6500..7138
                     /locus_tag="VF752_14140"
     CDS             6500..7138
                     /locus_tag="VF752_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8856733.1"
                     /translation="MASVPSTAETRQGRREERKAENREKLLAAARKVFAEKGVGAATA
                     RDIVRETDLASGTFYNYFRDKDEVFRALVEELTEKARAAVRAQRKQPGRTLEERIEGA
                     YRAYFQLVLEERELFIMLRRNAGVISQSGTQNLLESGIGDLVEDMAEWEEAGDLPRVD
                     LDYLATAMVGAAFYIAGHLVERQPPDVEAATSFCARLFVGGIRAVSPPAETG"
     gene            7145..9349
                     /locus_tag="VF752_14145"
     CDS             7145..9349
                     /locus_tag="VF752_14145"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX8856734.1"
                     /translation="MLIAGRALLILALAIAAYGIFASLYGTRTRNTGWVASGRRSMYA
                     LATTVAVCFGVLEAAFLRSDFSFELVATHSSTTTPAFYRATAAWSSQEGSLLLWVMLL
                     SGWSSLILFLAQRRLRRVAPYATAVLLGLAAFFLGLLVFLASPFSRLSPAPIEGSGLN
                     PILQHPAMMIHPPMLYSGYTLFTVPFAFAVGALITRRLGTDWIRTVRPFALGAWLCLG
                     IGIVLGARWSYSELGWGGYWAWDGVENAALMPWLTGTAFLHSLMIQERRGMLKVWNVS
                     LVLATGVLAVLGTFLVRSGVLQSIHAFGASTLGVPFLVLISLMVAGSILLVVSRSAGL
                     RSEHRLDSLISREAVFLLNNLVLVGLCFVIFWGTFFPLISEAVTGNRASVGPPWFDRY
                     TVPLALLLVLLSGLGPLLAWRGTTPAKLWRLLRVPVSASAVTLVVLLAAGGVAEKPTS
                     LLMFCLAAFVLAVVAQEFWRGARARSALSQEPLPVALVSLIRRNRHRYGGYIVHAGIA
                     VLFVGIAASSAFRDVRDVRLAPGQSARVGAYEVTYVRPTGKIDIASNGSLEKIRLGAR
                     LAVRRSGKATALEPSKSFFPSNEASLGAVSRYFEGEATSEVGMQSGLRRDVWTVVQPS
                     IGRIESIARRGDAVFREASSLPRAERAAALGEALRRLVASYANEPSPVSFRVLISPLV
                     AWIWLGAVIVFTGGLMTIWPAPSRARRRITASHAARLARGVGMGDPKARLGN"
     gene            9465..>11923
                     /locus_tag="VF752_14150"
     CDS             9465..>11923
                     /locus_tag="VF752_14150"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8856735.1"
                     /translation="MAVLLCLAVTDTAAGATFRAPTYSSPIALSRDGRLLWVVNPGGD
                     NVAVISTRSNRVVSRIAVGDEPQGLAIDPNNRYAYVANTAAGTVTIIRITNANPNRFR
                     ARVARDLGRRGTFLTGAEPWNIVSSPDGKRIFVANSSQDTITVIDATQSRSRSGRLTS
                     PRVIGGVALRGSACSPNPDFHFQPRGMAVSLDNKKLFVTSFFAFQRPGFRQVNDQGRQ
                     GIVCRLRINTSSDRIRSYRPAQRVGILPRDSGFTVDKTGDGVPDPIAAWPNQLQSIVI
                     RGGQAYLPNIAASPEGPQRFNSSTMAYVNVLDGVRSRTQTDGSGGRFLNLHLGARTPE
                     PGKKKLFFANPWAIGFTNQKGSGAAYVVSAGSDLLVKVNVNASGALSFTGGPMTTRYI
                     DLNNPADGATSGDNAGKNPQGIVINAKGTRAYVNNFVSRNVSVIDTRNDRVIRTIRVA
                     PLPPGGSMEELVAVGAEMFFSSRGNFNRPPGTTVSTSERLSSDAWQSCSSCHFKGLTD
                     SVVWAFGSGPRKSLPLNASFNPRNRAQQKMLNYSAINDEIEDFEVNIRNVSGPGPLAA
                     AQPCSEPAPGQPATSTFDPNHGLIFGDNGDINLAPCIVNQIPAKANANRNQFTVTLPG
                     SNTAVPALTAMREWVRFAVRTPHGPFTNRGLPGRLDPAAVQAGRALFAQQGCSGCHGG
                     TLFSNSIKDYTAPPPPADIFTERMPPPVTGNPVAAPYVARFLRDIGSFNLGVPGQGND
                     LGANIGADEKAAAALVNGVSQPPQDGLGRDYNNDGKGNGFTIPSLLGIDQLPPYYHNG
                     ACETLDCVVSNQKHRTALGSQP"
BASE COUNT         1717 a         4130 c         4219 g         1857 t
ORIGIN      
        1 gtcggccgcc gcggctggcc ttccggcggc cgcgacctca cccgccgctc gctgcgcggc
       61 aagcacggcg cgctcgagtg ccgacacgac tccgggcggc ggcggctcct gcctggtgcc
      121 gacggccgcg cgagcggcgg ccgtgcgccg gtagagcgcc cttgcggccg cgagcgccat
      181 ggcggcggtc gcgccgccct ccaccgcccg caggcttcgg cctctgtttc gcctcgccgc
      241 catgtgtcgg cgcccatgat cgttcacgga gtggacggct aatcggcggg caggccgcgc
      301 gacgaccact cgctccacga gccggggtag agccgggccc ggcggcccgc cacgtcggct
      361 gcgagcacga gcacgcacgc ggtcacaccg gagccgcagt aggcgacgag ctcactgctg
      421 tccggcagac gcgcatggat ctcgtccggc gcgggcatcg cgccgcccgg cgccagctcc
      481 gcgaacggca cgttccgcgc gcccgggatg tgcccggcca ccggatcgat cggctcgttc
      541 tcgccgcgga agcgctcggc cgcgcgggca tcgacgagcg ccagcgaccc gtcgccgagc
      601 cggcgctcga tctctaccgc ctccgcggtg tcgccgtccg ggcctgggga tggctcgaag
      661 tcgcctggcg cgatcacctc cgttcccgcc gtgagcgggc cgcccgccgc cgtccaggcg
      721 ccgagtgctc catcgagaac cgcaacgcgt tcgtggccgt gatggcggag cagccaccac
      781 agcctggcgg cgccgccctc gccctgctcg tcgtaggcaa tcaccgcgct gtcgcgcgag
      841 atgccggcgc gccgcgccga ctccgcgaat cgctccggat cgggcagcgg gtggcggccg
      901 ccctccgggc cgcccggtgg ccccgccagg tgccggttca ggtccatgaa tgccgcaccg
      961 gggatgtgcc cctcgaggta gccgcgttcg cccgctgccg gatcggtgag cgtgaagcgg
     1021 cagtcgacga cgcgcacgcg cgaatcgcgg gcgtgttcgc gcagccacga cgcgctcacc
     1081 agcgggccga tgctcatggt ggtcatgatt gcggttgtgc gcgcgatggt cctcgacaac
     1141 cccggcacgc cgcttcacga ggccgacctt ccggtgcccg agcccgggcc gggcgagctg
     1201 ctcgtgcgcg tgcacgcgtg cggggtctgc cggaccgact tgcacatcgt cgacgggcag
     1261 cttccgcatc cgaagctgcc gctcgtggtc gggcaccaga tcgtcgcgac ggtggaacag
     1321 gcgggcgacg gcgccgagcg gcatccggtc ggcacacgcg tggggatgcc gtggctttgg
     1381 tcagcggacg gaacgtgccc gtactgccgc agcggccgtg agaacctctg ccccaacgcc
     1441 cgcttcaccg gctacgacgt ggacggcggc tacgcggagt acacggtcgg gcgcgacgac
     1501 ttctgcctcg cactgccgga gggctatccc gacctgcagg ccgcgccgct gctctgcgcc
     1561 gggctgatcg gctaccgctc gctgcgcatg gcgggcgacg gcgagcgcct cgggctctac
     1621 ggattcggcg cgtcggcaca catcatctgc caggtggcgg tgcaccaggg gcggcgcgtg
     1681 ttcgcgttcg tgcgagaggg cgacgaggcg gccgcgtcgc tggcgctcga gctcggtgcc
     1741 gagtgggcgg gcgactcgat gaagcctgga cccgaggagc tcgacgccgc gatcatcttc
     1801 gcgccggccg gcccgctcgt gccggcggcg ctccgggcga ccgcccgcgg cggcatcgtc
     1861 gtgtgcgccg gcatccacat gagcgacatc ccatcgttcc cgtacgagct gctgtggggc
     1921 gagcgtgtgc tgcgctcggt cgcgaacctc actcgccagg acggcgtgga gttcctcgag
     1981 cttgcgccgc gcgtgccagt gcgcacgacg gtcaacacgt acgagctgga gcgcgcgaac
     2041 gacgcgctcg acacgctgcg cgcgggcgag atccgcggcg ccgcggtctt gacgctgtcc
     2101 tagagctacg ccgcgcgctg cagcggctgc acaggcttgc gtgtgcgcgc gtcgtcgcga
     2161 cggaagcgcg caagcgactc agcgatggtg cggtaggccg tcgggctgac cgccatgccg
     2221 cagtggctcg tctcgatctc gacgtgctcg gcgcactcct cgaggcaggc ctgccagtcg
     2281 acgatgccgt ccctcttcga atagaccgac acgtagccga cctcctccgg gaagggagtc
     2341 gcgaggtcct cccagaagca ctcgcagcac ctgccgttgc ggcaggagtg cttgaacaag
     2401 ccgcgcgcgc cgagcgtgcc gagcagcccg accgcgagga tcggcaccgt gaccagcggg
     2461 tggatcgcga aggagttcga gaccggcgag ccgagcgtga cgatgcccga cacgaggtct
     2521 ggcctgcgca cggcgagcac tcgcgcgatc gtgccgccac ggctctggcc gatgagcgca
     2581 acgcgctgtc cctgcttgtc ggcgagacac tcgacccgct cgctgagccg ccgcacggtc
     2641 gcctccgagc agtcaatgtt cgatcgcatc ccagcgctct tcgtgtggtg gccggtgcgc
     2701 cgcagccagt gggtcatcac gccgagggag tcgtcgcccg cgaggaagcc cgggatcagc
     2761 atcaccggct gcccgccggc cggcttcacg ccctcgcccc tgaagatggg gtgccgggtg
     2821 agctgagcga gctcgagccc gaccagtgcc tcacgccaga tcggtggctg tctgagaagc
     2881 gagggggttc tcatatcgtt gcgatgccct taatgacgcc ggcgtcaaac ttgaatccgc
     2941 gcctgaagct atatccagcg cggcggctgg aagcccggtt tccgcttcgc cactaggatc
     3001 cgccgccgtg cgggcagcca cgatccgaga cgagcagatc gtcctggagg accaccctga
     3061 tccttcgccg ggcaagggcg aggtcctcgt tgaggtgcac gccgccgggg tgaacggggc
     3121 cgacctgctc cagcggaagg gcctctaccg ggcgccgccg ggctcgcatc aggacatccc
     3181 gggactcgag ctcgccggta cggtcgcggc gctcgggcca ggtgccacgc gcttcaagga
     3241 gggcgatcgc gtgatggcgg tggtcggagg cggtggccag gctgagcgcg cggtcgtgca
     3301 cgagcgccag ctcatgccgg tgcccgagtc gctcagctgg gcggaggcgg gcggcgtgcc
     3361 cgaggtcttc accacggcgc acgacatgct gttcacccag ctcggcctcg gcccgggcga
     3421 gcggctgctc gtgcatgggg ccgccggcgg cgtgggcacc gcagcggttc agctcggccg
     3481 cgccaccggc gcacgcgtga ccgcgaccgt gcgaaacgag agcctgcgcg acgcggtgga
     3541 ggagctcggc gcgggaggcg tgatcccgcc cgaggtcttc ggggaggccg gaccgttcga
     3601 cgtgatcctc gagctggtgg gcgcgcccaa catgcccggc aacctgacgg cgctcgagaa
     3661 ccgcggccgg atcggcgtga tcgggatcgg ggcaggcgcg aaggccgaac tcaacctcgg
     3721 cctcgtgctc ggcaagcgcg cgaccgtgtg cggctccacg ttgcgtccgc ggcctctcga
     3781 ggagaaggcg gacgccgcgc gccggatgga gcgccaagtg ctgccgctgt tcgacaccgg
     3841 cgcgctccgc gtgccggtcg cggacacgtt cccgctcgac cgggcggccg aggcctacga
     3901 gcgcttcgag gccggcggca agctcgggaa gatcgtgatc gttacgcggt agccgcggcg
     3961 gcctgctcgg cgccgctctc gcgcgcgatg ccgtcgatcg cggagcccag ctggtagctg
     4021 atgatgcgcg cgcggttgat ctcgctgcgg gtccacgggc gctcggtgcg gctcagcgcc
     4081 tcgatcagcc caagcgtctc gccgcggcac acgaccggga ccatgagcag cgcggcgaag
     4141 ccggtgcgct gcaggagcgc gacctcgccg aggtctgcgc ccgggtcgga cacgagcacc
     4201 tgcacggcct cctgcgtggt gagcacgtgc ctggtggagg ggtagttgct cagcttgaag
     4261 cgctcgccgg cgggcgcctg gtcgccgccg cagatcgtct cgacggagtc gtcctcgggc
     4321 agccagcggg acacggtcac atcgtccgag ccgacctcgg cggcgatcag ctcgagcagc
     4381 tcgccgagct catcgagtga gccgacctgc gagatgcggg cgctcacgaa ctcgaggcgc
     4441 ttgtcgccgg agtccgtcgg gtcgctgtcg gcggtcacgt cggcctggct ctgcaggctc
     4501 cggcgcagca gcgcctcgga gactgcgctc gacacggagg tccagtcgtc ggccgggcgc
     4561 gcgagggcgt acccctggcc gtaggtcacg tcgaggtcgg cgagcgtccg cagctcttcc
     4621 atcgtctcga tgccctcggc gcacaccgtt gcgccggtgc ggcgcgcgaa gcgcacgaac
     4681 gagtcgatca gcgcaacctt ggccgggtcg tcgttgagcc ccatcaccag cgatcggtcg
     4741 agcttgatca cgtccgggtg gacgagcatc acctgctgga ggccggagta gcctgagccc
     4801 gcgtcgtcga cggcaatccg cgcgccgcgg tcgcgcagct tctgcagctc ggcgagcacg
     4861 gtgtcgatgt cgtccgccag ctcgtgctcg gtgatctcga ccacgaggtt gctcatgtcc
     4921 tccggcagca cctcttggat ctcgggtgtc gcgatgatcg tcgggctaac attgatcgag
     4981 aggaacgtgc cggccgggcg gttttggtgt tcgagcgcgg cctcgatggc catcgcctcg
     5041 agcttggcgc cgagattgca tcggtgcgcc tgcgcgaacc acgcgtccgg gccgcgctgc
     5101 ggcgggtgcg ggaagcgggc gagcgcctcg aagcccagga tcttgcccgt ggagagagcg
     5161 acgagcggct ggaagaaggg gacgatcggc tcctcgagct cgaacagcgc ctccacctcg
     5221 gcgagctcgt cctcgccgcg cggcacgacc acgacctcgg ggtcgtaacg gcggctctgg
     5281 ttcttgccct cgcgcttcgc gagacggagc gcgccgttcg cgaactgcag cagcgtgctc
     5341 tcgtcgcggg cgtgatccgg gtagcaggcc acgccggcgg aagaggtgag gcggcgcccg
     5401 ttcggcgaga gctcggcgat cacgctacgc gcacgctcgg cgatcgagta ggccacgtcg
     5461 ggatcggtgc ccggcaggat cagcgcgaac tcgtcgccgc cgaggcgcga gagcacgtcg
     5521 gtcgcgcgca gcatgtcgcg cagctcctcg gccacgttgc gaaggacctc gtcgcccttc
     5581 gcgtggccgt agttgtcgtt gatcgactcg aagcggtcga ggccgagcac cacgaggccg
     5641 acggagttct gttcgcgctg cgcgcgcttg atctcgatcc cgagcgtctc ctggaactgg
     5701 cggttgctcg cggcgccggt gaggtagtcg gtgttctccg ccttctctgc gttggccgtg
     5761 ctctcgacct gcgcctgcag gaggtcgttg acccggccgg tcacggcgac gatctcggcg
     5821 ggcccctcaa cggccgcgcg cgcctcggtg tcgccagcca gtcccttcgc cacggcatcg
     5881 gcgagcgagc gcagcggggc ggcaaggcgg cggtcgagca gcgaggcgat cagcgggaag
     5941 aacacgagga acgcacccag tccgatgccg agcgtcttca cgagcgccga ggtggtggcc
     6001 ttgccgatgg cgtcggaggg gacgctcaca gtgagcgtgc ccggcgggtt ggtgagcctg
     6061 tacgtgaagg tctcgtggcc gccctgctgc ggcgcgaggg tagcgttcgc acccttctcg
     6121 ccgaacgcga cgcccgcgcc ggaagcggcg gcctcggcga gcgcgcggcg gacggacgcg
     6181 gggccgggga gccggcgcga cggcctcgcg gcctgctcga aggtggcgcg ggcggcctcg
     6241 gcgcgtgcag ccacgccgtc cttggcggcg ttgcgcgcag agagcgtgca ggccagcccg
     6301 acgcacagcg ccacgagcgc gatcgcgacg gcgagcggaa ttaggacctg gctgcggaga
     6361 gactgcccag aagttcgcac gatgcggttc ttcaccccgt ggcggtatcg acatgtgccg
     6421 tcccccgctt tagccctggc tctgaccacg tggtcagaag ggcttgaccg cttctcccaa
     6481 cccgatgtac aaagccgtaa tggcaagcgt tccgagcacg gcggagacac ggcagggcag
     6541 gcgcgaggag cgcaaggccg agaatcgcga gaagctgctg gccgcggctc gcaaggtgtt
     6601 cgcggagaag ggcgtcggcg cggctaccgc gcgggacatc gtgcgcgaaa ccgacctcgc
     6661 gtccggcacc ttctacaact acttccgcga caaggacgag gttttccggg cgctggtgga
     6721 ggagctcacg gagaaggcgc gcgccgcggt acgcgcgcag cgcaagcagc cgggccgcac
     6781 gctcgaggag cgcatcgagg gcgcgtatcg cgcgtacttc cagctggtgc tcgaggagcg
     6841 ggagctgttc atcatgctgc ggcgcaacgc gggcgtgatc tcgcagtccg gcacccagaa
     6901 tctgctcgag tcgggcatcg gcgacctcgt ggaggacatg gcggagtggg aggaggcagg
     6961 cgacctccca cgcgtcgacc tcgactacct cgccaccgcg atggtcggcg ccgcgttcta
     7021 catcgcaggc cacctcgtgg agcgtcagcc gcctgacgtg gaggcggcta cgagcttctg
     7081 cgcccgcctg ttcgtgggcg ggatccgggc ggtctcgccc cccgccgaaa cgggctgagc
     7141 cggcatgctg atcgccgggc gcgctctcct gattctggcg ctcgcgatcg cggcctacgg
     7201 gatcttcgcg tcgctgtacg gcacacgcac gcggaacacg ggctgggtgg cctcgggcag
     7261 acgctccatg tacgcgctcg cgaccacggt ggccgtgtgc ttcggcgtat tggaggctgc
     7321 gttcctgcgt tcggacttct cgttcgagct tgtggcgacg cactcgtcaa ccaccactcc
     7381 ggcgttttac cgcgcgaccg ccgcgtggtc gtcgcaagag ggttcgctgc tcctgtgggt
     7441 gatgctcctc agcggatggt ccagcctgat cctgttcctc gcccagcgcc gcctgcgccg
     7501 ggtggcgccg tacgcgactg cggtgctgct cggcctggct gcattcttcc tcgggctgtt
     7561 ggtgttcctg gcctcgccgt tctcccggct cagccccgcg ccgatcgagg ggtcaggcct
     7621 caacccaatc ctccagcacc cggcgatgat gatccacccg ccgatgctgt attcggggta
     7681 cacgctgttc acggtgccct tcgcgttcgc cgtcggcgcg ctcatcacgc ggcgcctcgg
     7741 gacggactgg atccgcaccg tccgtccgtt cgccctaggc gcttggctct gcctcggcat
     7801 cgggatcgtg ctcggggcca ggtggtccta ttccgagctc ggctggggcg gctactgggc
     7861 gtgggacggc gtcgagaacg ccgcgctgat gccgtggctc accggcacgg cgttcctcca
     7921 ctcgctcatg attcaggaac ggcgagggat gctgaaggtg tggaacgtct cgctcgtgct
     7981 tgccaccggc gttctcgccg ttctgggcac gttcctcgtg cgctcgggtg tgctgcagtc
     8041 gatccacgcg ttcggcgcct caacgctcgg tgtccccttc ctggtcctga tctcgctcat
     8101 ggtggcgggc tcgatccttc tcgtggtttc gcgctccgcc ggcctgcgct cggagcaccg
     8161 cctcgactcg ctgatctcgc gagaggcggt gttcctcttg aacaacctcg tgctggtcgg
     8221 actctgcttc gtgatcttct ggggcacctt cttcccgctg atctcggagg ccgtcacggg
     8281 gaatcgagcc tcggtcgggc cgccgtggtt cgaccgctac accgtgccgc tcgcgcttct
     8341 actcgtgctt ctctcaggtc tcgggccgct gctggcgtgg cgcggcacga cccccgcgaa
     8401 actgtggcgg ctgctgcggg tccccgttag cgcgagcgcc gttacgctgg tggtgctgct
     8461 cgcggctggg ggagtggcgg aaaagcccac gtcgctcctg atgttctgtc ttgccgcgtt
     8521 cgtgctcgca gtggtcgctc aggagttctg gcgaggcgcc cgtgcccgaa gcgcgctgtc
     8581 gcaagagccg ctgcccgttg ccctcgtgtc gctgatccgc cggaaccggc accgatatgg
     8641 cggctacatc gtgcacgccg ggatcgcagt gctgttcgtt ggcatcgccg cttcgtcggc
     8701 gttccgggac gtgcgcgacg tgcgcctcgc gccgggacag agcgcgcgcg tcggcgcgta
     8761 cgaggtcacc tacgtccggc ccacggggaa gatcgacatc gcgtcgaacg gatcgctcga
     8821 aaagatccgc ctcggcgctc ggctcgccgt ccgccgaagc ggcaaggcga cggcgctcga
     8881 gccctcgaag agcttcttcc catcgaacga ggcctcgctc ggcgccgtct cgcgctattt
     8941 cgagggcgag gcgacaagcg aggtgggcat gcaatcggga ctgaggcgcg acgtctggac
     9001 ggtggtccag cccagcatcg gcagaatcga gtcgatcgct cgccgcggcg acgccgtctt
     9061 ccgcgaggcg agttcgctgc ctcgtgccga gcgcgcggca gccctcggtg aggcgctccg
     9121 ccgcctcgtg gcaagctacg cgaacgagcc atcgccggtc agtttccggg tgttgatctc
     9181 tccgctcgtc gcgtggatat ggctcggcgc cgtcatcgtc ttcaccggcg ggctgatgac
     9241 gatctggccg gcgccgtccc gcgcgcggcg caggatcacc gcgtcgcatg cggcgcgact
     9301 cgctcggggg gtcggtatgg gtgaccccaa ggcgagattg gggaattgac tcggtttttc
     9361 cagagtggtt aggtcgggtg tcgcaaggcc cccgattcga ggaacctgtg cagcgtccgc
     9421 aagccagggc cgtttcggat cgccggacga tcggtattgc cgtcgtggcc gtgctgctgt
     9481 gccttgccgt cactgacacg gccgcgggag cgaccttccg tgctcccact tactccagcc
     9541 cgatagcgct gtcgcgtgac ggccgcctgc tctgggtcgt gaacccaggc ggtgacaacg
     9601 tggctgtgat cagcacgagg agcaacaggg tggtcagccg gatcgccgtg ggagacgagc
     9661 cgcaggggct cgccatcgac ccgaacaaca ggtacgccta cgtggccaac acggccgcgg
     9721 gcacggtcac gatcatcagg atcaccaatg caaacccgaa ccgcttcagg gcgagggtgg
     9781 cgagggacct cggcaggcgg ggcacgttcc tcaccggcgc ggagccttgg aacatcgtct
     9841 cttctcccga cggcaagcgg atcttcgtgg cgaacagcag ccaggacacc atcacggtga
     9901 tcgacgccac ccagagccga tccaggagcg ggaggctcac cagcccgcgc gtgatcggcg
     9961 gagtcgccct gcgcggcagc gcctgcagcc cgaacccgga cttccacttc cagccgcgcg
    10021 ggatggcggt gagtctggac aacaagaagc tgttcgtcac aagcttcttc gctttccagc
    10081 gcccgggctt ccggcaggtg aacgaccagg gccggcaggg gatcgtctgc cgcctgagga
    10141 tcaacaccag ctcggacagg atcaggtcgt acaggccggc ccagcgggtg ggcatcctgc
    10201 ctcgggactc gggcttcacc gtcgacaaga ccggtgacgg cgtgcccgac ccgatcgccg
    10261 cctggccgaa ccagctgcag agcatcgtga tacgcggcgg ccaggcgtat ctgccgaaca
    10321 tcgccgcctc gccggaggga ccgcagcgct tcaacagctc gacgatggcg tacgtgaacg
    10381 tgctcgacgg agtcaggtcg aggacccaga ccgatggcag cgggggtcgt ttcctcaacc
    10441 ttcacctagg cgctcgcaca cctgagccgg ggaagaaaaa gctcttcttc gcgaatccct
    10501 gggccatcgg cttcaccaat cagaagggat ccggcgccgc gtatgtggtg tcggcgggct
    10561 ccgaccttct cgtgaaggtg aacgtgaacg cgagtggcgc gctcagcttc accggcggcc
    10621 cgatgacaac tcgctacatc gacctgaaca acccggcgga cggcgcgacc agcggggaca
    10681 acgcgggcaa gaacccgcag ggcatcgtga taaacgccaa gggcacgcgc gcctacgtga
    10741 acaacttcgt gtcgcgcaac gtgtcggtga tcgacaccag gaacgacagg gtgatcagga
    10801 cgattcgcgt ggcgccgctg ccgcccggcg ggtcgatgga ggagctcgtc gccgtgggcg
    10861 ccgagatgtt cttcagctcg cgtggcaact tcaaccgccc gcccggcacg accgtctcca
    10921 cgagcgagcg tctgtcgagc gacgcctggc agagctgctc gagctgccac ttcaagggcc
    10981 tcaccgacag cgtggtctgg gcgttcggtt cgggaccgcg aaagtcgctg ccgctaaacg
    11041 ccagcttcaa cccacggaac cgcgcccagc agaagatgct caactactcc gccatcaacg
    11101 acgagatcga ggacttcgag gtcaacatcc gcaacgtgtc gggtccgggc ccgcttgccg
    11161 cggcgcagcc gtgcagcgag cctgcgcccg gccagcccgc cacgagcacc ttcgacccga
    11221 accacggact gatcttcggc gacaacggcg acatcaacct ggcgccctgc atcgtcaacc
    11281 agattcctgc gaaggcgaac gccaaccgca atcagttcac ggtcacgctc cccggcagca
    11341 acacggccgt gccggcgctc accgccatga gggagtgggt gagattcgcc gttcgcacgc
    11401 cgcacgggcc gttcacaaac cgcgggctgc ccggccggct cgatccggct gcggtgcaag
    11461 cgggccgggc actgttcgcg cagcaggggt gcagtggctg tcacggcgga acgctgttct
    11521 cgaattcgat caaggactac accgcgccgc cacccccggc agacatcttc actgagcgca
    11581 tgccgcctcc tgtgacgggc aatcctgtgg ctgctccgta cgtggcgcgg ttcctccggg
    11641 acatcggctc cttcaacctc ggcgtgccgg ggcagggcaa cgacctcggc gccaatatcg
    11701 gcgccgacga gaaggcggcc gcggcgttgg tcaacggggt ttcgcagccg cctcaggacg
    11761 gcctgggccg cgactacaac aacgacggca agggcaacgg cttcacgatc ccgtcgctgc
    11821 tggggatcga ccagctgccg ccctactacc acaacggtgc ctgcgagacg ctcgactgcg
    11881 ttgtctcgaa ccagaagcac cggaccgccc tcggatcgca gcc
//
LOCUS       DASVXS010000249        24068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1095307, whole genome shotgun sequence.
ACCESSION   DASVXS010000249 DASVXS010000000
VERSION     DASVXS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 24068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24068
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095307"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VF752_14155"
     CDS             <1..549
                     /locus_tag="VF752_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX8856736.1"
                     /translation="HKWLNVPYDSGFVFVRDWSALQRTFGERGDYLPPLDDPNPSFAY
                     VAPEGSRRARALAVWATLRAYGADGYRGMVERHAHLARHLARLVDEHPALERVAEVTL
                     NIVCFRVKPEGVPEERLDGLNRRVGEELLADGRVFAGTTIYDGKVCFRPAIVNWRTRE
                     SDVELLLDVVLELSERLQAAAV"
     gene            complement(546..1688)
                     /locus_tag="VF752_14160"
     CDS             complement(546..1688)
                     /locus_tag="VF752_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856737.1"
                     /translation="MKRLAPLLAVPILLALVAPAALARDPGRWAMLRHDPFPGGYNQG
                     ITSNGRSLFFDGALGASYAALYRNSLALTQRRGNTLIIPQDVRLREGYNHIGDIGFVR
                     SGGGRVLLPLECYTPGAPNGGNTCGTGSIAVADPSTLAWRYYVKLDPAGIPKAMWVEP
                     SPDGRLLWTSARKDLLAYRTADVTAANAAPAAAPIRAVKRLKRAVPPSGITGAVFHDG
                     RLFVAGSDAGPFQIWSVDLTTGKRRLEFERRFNGESEGLDVGPYMGGLLHWMILPFDS
                     GPPTFGNQGALLELVPAGRYAIRLTGPRVIGEGLRTVEFRASYRLGRRRWPLEGARVT
                     AGGKSGITQADGHVTLTIRLKEGVNPAVATFRGLLPGTFALTARPN"
     gene            1712..2164
                     /locus_tag="VF752_14165"
     CDS             1712..2164
                     /locus_tag="VF752_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8856738.1"
                     /translation="MTLERVTESELPELLPLMRAYCDFYEVQPSDDALLAMSRELIAD
                     PEKEGVQLLARDDDGNAVGFATIFWSWSTSDAARIGVMNDLFVAEAARGGGAAEALIR
                     ACVDECRARGAGRLTWQTALDNHRAQAVYDRVGGVREHWVDYWLEAPA"
     gene            complement(2140..3420)
                     /locus_tag="VF752_14170"
     CDS             complement(2140..3420)
                     /locus_tag="VF752_14170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8856739.1"
                     /translation="MAPVPEYDVAIVGASIAGCTAAILYGRAGARVALIESHTDPARF
                     KRVCTHYIQPSAAEGIERLGILPDLEAEGATRTHIDLWTKWGWVVPAEDDEPRAYNLR
                     RAKLDPLLRRIASETEGVRLLLGESADGLLRHGARFAGVRTKSRDGSSREITARLVVG
                     ADGRDSKVGELAGIPAKVSTNNRFGYYANFSGVKMRRPGVAQMYLLDPDVAYAFPTDD
                     ELTTVALFMHKERLAEFKADMEGTVRRLFDSLPEGPRLDGAERETPWIGKLEMPNLRR
                     RASAPAIAFIGDAALAADPLWGVGCGFAFQSATWLVDATADAVHAEGDLDDALKRYRK
                     RHTAGLAAHHRAISTYSTGRRMNLFERALFSAATRDLDAARHMRRYGDRRIVPKELLA
                     PSQLAHMVRVNLRYALTHRNGAEAAGAQAGASSQ"
     gene            3539..4039
                     /locus_tag="VF752_14175"
     CDS             3539..4039
                     /locus_tag="VF752_14175"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX8856740.1"
                     /translation="MRVGHRWLVDEERNDTGGEPNESEKERIDRELIELLNELRVALP
                     GVQVLFAFLLAVPFQQRFATVTAFQRDVYYGTLLATTISAAMLIAPSAYHRLNFRRQD
                     KRRLLVHSNRLAIGGLAALAVAMCCVVLLITDFLFSTTLTIVSTALAFTLFAVLWVVM
                     PLWRRR"
     gene            complement(4036..5049)
                     /gene="nrfD"
                     /locus_tag="VF752_14180"
     CDS             complement(4036..5049)
                     /gene="nrfD"
                     /locus_tag="VF752_14180"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX8856741.1"
                     /translation="MSTRPEVPSATLETRPRRNGKGERAMVPEAEPRSYYDQPVIQEP
                     VWTWEIPIYFFVGGMAGASAGLAYMAHLQGNNELSRRAWAISLAGIHVSPMLLISDLG
                     RPARFLNMLRMFKITSPMSVGSWILATTGGAVTVSAAHAWTGVFPGLARSGRLVAALA
                     GMPLATYTGALIGNTAVPAWHKARHFLPFVFAGGAAASAGAAAVLATPGKHAEPARRL
                     AVIGAALEIGAAQGMEHAAGFEGEPYKKGLPGALSKLATATTAAGAGLLAKGGRRRSD
                     RTLAKVGAGLLLAGAFTERWAIFRAGFKSASEPKYTVGPQRERIRKGESLGAARMPGN
                     RPG"
     gene            complement(5046..5972)
                     /locus_tag="VF752_14185"
     CDS             complement(5046..5972)
                     /locus_tag="VF752_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8856742.1"
                     /translation="MSSNAKLLTLDAGAWPDTYGEMAKPRMGFFTDTSICIGCKACEV
                     ACKEWNHVPEDFKGFTGKSYDNTLHLDADNWRHVAFIEQRLPAALDGDTATESLVQIA
                     ARRELEEDSGLDTLQEGEGLRWLMASDVCKHCSHAACLDVCPTGALFRTEYGTVVVQA
                     DICNGCGYCVPACPFGVLSKRKPDGRVWKCTLCYDRLRDDKEPACAQACPTNSIQYGE
                     LTELHERAEARLQEMQDAGQTSARLYGAHPDDGVGGFGAFFLLLDEPEVYGLPPDPVV
                     TTRDLPGIWASTGAAAVALAVGIAAACIGGRR"
     gene            complement(5969..9229)
                     /gene="fdh"
                     /locus_tag="VF752_14190"
     CDS             complement(5969..9229)
                     /gene="fdh"
                     /locus_tag="VF752_14190"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8621..8623),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX8856743.1"
                     /translation="MPIRRGVPRRRALEEGNGKLTDHLPPLLRQLKDPLGLGRAAQSR
                     HSARLVPRTQTADKVVQSVCPYCAVGCGQKVYVQDGKVTQIEGDPDSPISRGRLCPKG
                     AASEQYVNSATRAKKVLYRAPYATDWQEIPLDQAMEMITDRVIATREKTWESEDDEGR
                     PLNRTRGIAWLGGATLDSEENYLIKKLFTALGAVQIENQARIUHSSTVPGLGTSFGRG
                     GATTFQQDLQNSDVIVIQGSNMAECHPVGFQWVMEAKQRGAKVIHVDPRFTRTSAMAD
                     LHVPIRAGTDIAFLGGIVNYIIENERWFDEYVKHYTNATTIVDEGFRDTEDLDGFFSG
                     WDPETGQYDISTWSYAGTEEHTAGGHKEQGAAVSGEQAHGAHGMKLDRGRPPKEDETM
                     QHPRCVFQILKRHYSRYTPELVEEVCGIPKETFLQVAETLCQNSGRERTGAFCYAVGW
                     TQHTVGVQYIRTAGIIQLLLGNIGRPGGGILALRGHANIQGSTDIPTLFNILPGYLPM
                     PHPQAGPDLDTWVGNTSPRTGAWGDMRNYAVSLLKAWFGDAATGENDFCFGLLPRIDA
                     DHSHYAAINRMLDGKCPGFFVVGENPVVGSANGRMQRFALANLEWLVVRDVQLIETAT
                     FWKDSREIETGELKTEEIGTEVFFMPAASHTEKDGSFTNTQRLLQWHWKAVEPEGDAR
                     SELQWTFDLGRRVRRKLAGSTEPRDRALLNLTWNYTTQGPHDEPDAQEVLQEIGGRHA
                     DGSFVEKYQDLKNDGSTSCGSWIHAGVYKGGVNQAARKKPHTEQNWVAPEWGWAWPAN
                     RHIIYNRASADPSGKPWSERKKYVWWDEEQQKWTGYDDPDFVPDKPPDYQPKKNDIGL
                     DALRGDSPFIIQADGRGWLYAPAGLMDGPMPTHYEPHESPATNPLYTQQQNPLRQRFN
                     RPENPYNPTDPEPGWDVFPYVVTTYRLTEHHTAGGMSRTVPILSQLQPELFCEISPEL
                     AAERGIEHGDWATIVTSRTAIEARANVTDRVKPLRVEGRTIHQVGLPYHWGPGGIVKG
                     DTANDLFPLVLDPNVHIQEVKAATCDILPGRRPRGPDVLRLVEDYKRRAGVKVVGE"
     gene            complement(9246..9614)
                     /locus_tag="VF752_14195"
     CDS             complement(9246..9614)
                     /locus_tag="VF752_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856744.1"
                     /translation="MRRLRLAGGGAGREGAPGRTAAMTAAMGRGGRRALNTLAGLVTL
                     TAGLVALVIVIGIAFVVLEANKDNAIVNAINDAASWLTQPFHNMFNPEKHKTEIVVNW
                     GLAALVYVIAGNVVARLLRR"
     gene            9718..10230
                     /locus_tag="VF752_14200"
     CDS             9718..10230
                     /locus_tag="VF752_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856745.1"
                     /translation="MAEISHQTVRLSRGKHAFPAQGVCVMELASMLAGEDFSDAPECV
                     SPVIGGFLRTYNDGIDDRLRQDLYEFAAKAVDTRSRASVEQRRASDCIAWAASLAGSL
                     TLRFPVFACTTSLRGCEAAGAYAARAALRQGPAAHAEALEFVERLMGLCEADKRPAPA
                     AERPVAAVAS"
     gene            complement(10227..10850)
                     /locus_tag="VF752_14205"
     CDS             complement(10227..10850)
                     /locus_tag="VF752_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856746.1"
                     /translation="MNTPHLAELVIADAPERWEALGFAVAGDAAQIGSVRVVFDPDAG
                     SGIARWAIRGLEQAELDGLPTVIANGSDPVPAPGRHPNGALSIDHVVAMTPDLDRTFA
                     ALERAGLDLRRVREATAPGGQPIGQGFYRLGEALLEVVGPDGRDADHPASFWGLTVTV
                     EDLDRCRRSLGELLGEPHDAVQPGRRIASVRRAAGLATALAFMSPPS"
     gene            complement(11104..12123)
                     /locus_tag="VF752_14210"
     CDS             complement(11104..12123)
                     /locus_tag="VF752_14210"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX8856747.1"
                     /translation="MRVYLTGATGFVGSNIARVFTERHGAELFCPVRESRPPAGAPYD
                     WAVTDVRDASAVAGSVRSARPDVIVHAAILNDFRRLYTDRRDSWASYVVATRNLVDAA
                     NAVGAHVVLVSTDWVFDGTQSGATEDTPPNPINLYGFLKAASELVVTERAEGGAVARI
                     SGVNGVHWARPETPRRQDAGFGYLVASIAEALGAGQRFTVWQSPEINTVATPTLASDA
                     AESLWRLAELGREGVFHCCGGESVDRAALARLTAEELELDPELLDFGPPDPEQLPPAP
                     VPYDTSLDSRATAAALGTERVDVRTMLRRMRFQLEHGELGPAAAPAQEGAQEPAAARL
                     PRLST"
     gene            complement(12126..13199)
                     /gene="iolG"
                     /locus_tag="VF752_14215"
     CDS             complement(12126..13199)
                     /gene="iolG"
                     /locus_tag="VF752_14215"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX8856748.1"
                     /translation="MPAAGQTRTGPRRLSSRQHSQTPLRIGVIGVGRIGSMHAELLAT
                     QVDGAELAAVHDADEEASRAAGRDFGVRACASVAELLDSRDLDAVAICSSTGTHPDFI
                     VAAANAGKPIFCEKPISLELAEVDRALAAVDAAGVPFQIGFNRRFDPAHQSVRDAVAN
                     GIVGDPHLVRISSRDPAPPPIDYIRASGGIFLDMTIHDFDMARFVTASEVTQVSAHGA
                     VRVDPAIADAGDVDTALVTLVHDNGCLTAIDNSRQAVYGFDQRVEVFGSRGMAASDNP
                     SGHTGVVRTADGAHGAPLPYFFLDRYVPSYVREWEAFVGAVRTGETPAVTGADARAPL
                     VIGLAAKRSLEEGRPVALDEVDR"
     gene            complement(13199..14440)
                     /locus_tag="VF752_14220"
     CDS             complement(13199..14440)
                     /locus_tag="VF752_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8856749.1"
                     /translation="MTHAPEETRSQAGAGRVGPPGRARRRIGVGLIGFGWLGQAHSRS
                     LQRVRTLFPEREFDPELVVCSDTVPARLEEAVGSFGYREGVADWRRVVDTPDVEAVFI
                     AAPNSLHLELIEAAAQAGKHVFCEKPVGGTPEQTARAERIARHAGVNTGVGYNYRWAP
                     LVRYAAELISSGRLGEITNYRGRFFSMYGSDPMGLLSWRFLAEEAGHGVTSDLLSHAV
                     DLAHMLIGPVTRVCGSVATFIPERPLPGAGGASHYGRGRPEDPRGAVTNEDYASMLCE
                     FENGARGTFEASRTIVGPESQMAFDVYGTGGAVGWNQERLNELQVYAASGELHTGYTT
                     VFGGDRFPYHGNFVPGSANGIGFEDLIVIEDYEFCRSLAEGRPHRPGFAEALEWVSVQ
                     AALLESARTGQWQPVVSLRQD"
     gene            complement(14437..15255)
                     /locus_tag="VF752_14225"
     CDS             complement(14437..15255)
                     /locus_tag="VF752_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8856750.1"
                     /translation="MTATQTDGNHALLEVRGVSKYFGNVIAIKDVSAAVGAGEVTCVL
                     GDNGAGKSTFIKILSGVHKQDEGELYMNGDKVSFASPRDARERGIATVFQDLATVPLM
                     SIWRNFFLGSEPEKKIGPFGFLDVNSAKRIMREELKKMGIDVRDPDQPVGTLSGGERQ
                     AVAISRAIYFGAKVLILDEPTSALGVKQSGIVLRYIAQARDRGVGVIFISHNPHHAYP
                     VGDRFVILNRGHLQGSWKRGEISRDELVRMMAGGAELDQLTHELERAPEVQTTQ"
     gene            complement(15252..16325)
                     /locus_tag="VF752_14230"
     CDS             complement(15252..16325)
                     /locus_tag="VF752_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8856751.1"
                     /translation="MSDQATTVPAPTPPAPVSVVDERLAHVSLLTKLLRRPELGALMA
                     ATVIGIFFGVTTSEFGTLDGAANWTDVASTLGIPAVAVALLMIGGEFDLSAGVMTGSS
                     GLFMGILATEVGMSIWPAILLTLVAAAMVGFINGYMVVRTKLPSFIVTLATFFVLQGV
                     NLGVTKAITGTVRVAGIGDVSGYDSAFKVLGGSFWSPHDFRVTVLWWIAITAIATWVL
                     ARTRVGNWIFGVGGDQVAARNVGVPVSRVKIALFMTTATASALVGIMIALRLASVQAG
                     QGIGDEFNYIIAAVVGGCLLTGGFGSAFGAAIGALIIGMAFIGIQFEGWNTDWRFLFL
                     GVILLLAVLINNFVRRSAEGARR"
     gene            complement(16389..17381)
                     /locus_tag="VF752_14235"
     CDS             complement(16389..17381)
                     /locus_tag="VF752_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX8856752.1"
                     /translation="MRRLFGPVAVLVLALAVAAFAVGCGGGNDNNGSTGDNVSATQGK
                     NLTFAVITHGEGDTFWAVAKKGAEQAGKDLGVKVNYSESQNDPQKQAQLIDAAVTQKV
                     DGIAVSAPNPDAIADPLKKAEDAGIPVITLNSGQNDSAKLGAITHVGQDESIAGEAAG
                     KRLADAGGKKLLCVIHEQNNIGLNQRCDGAKKGFGGTVENVQVKGVNDIQTSLTEIQS
                     KLRSDSSIDSVLTLNPDIAVAARDAIKGANSKAKLATFDLSGDVVKAIKSGEIEFAVD
                     QQQYEQGYLPVVFLTLYKTNNNTVGGGKPVLTGPGFVDKSNAGTVEKLAGEGTR"
     gene            17688..18668
                     /locus_tag="VF752_14240"
     CDS             17688..18668
                     /locus_tag="VF752_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8856753.1"
                     /translation="MDDVARKAGVSRALVSLVMRESPKVSPERRGRVLAAASELGYRP
                     NAMARSLASRRTRTVGVLLNDLHNPFFAEIADGIEELASELGYRLLITSGGRRQRREQ
                     TMLEGLLEFRTDGLILVSPRMRTAEMLAATGGAPVVVVSRVVRSDSVDCVTTDESLGA
                     RLAVRHLVELGHKRIVHVDGGQGAGSAPRRGGYLRAMREVGLEQYAQVIAGDFTDAAG
                     VRAAEQLLESPELPTAVFAANDLVAAGVLDRLEDAGVSVPGDVSIVGFDNTFLAALHH
                     MSLTTIDQPRPEMGRLALELLLERMDGREPPAMRVVEPTLVVRSTSGPPR"
     gene            18665..19303
                     /locus_tag="VF752_14245"
     CDS             18665..19303
                     /locus_tag="VF752_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8856754.1"
                     /translation="MSALRLGALACLALVAAAVAIVATRSSTPQHGPQSSPTVVTVLA
                     RSGTAAEEKATGFAVANERVVTVAHALSGSAVAEVLDQRGVVHRGSVVRRDARLDLAL
                     LSVPGLRAGEMQPAASTGRARVLVMRGSDVARLPADIRRHVAFHLVDRVAARTYDRPA
                     LELAARIDAGDSGAPVLAGGRVAGVVFGRSNIRDGTAYAVDAEALNRLVSPR"
     gene            complement(19415..19846)
                     /locus_tag="VF752_14250"
     CDS             complement(19415..19846)
                     /locus_tag="VF752_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8856755.1"
                     /translation="MARIQRDQKISEIMTGDPVTVERDTPIAEVARQMRDADTGAILV
                     TDNGRLFGIATDRDIAVRAVADGLDPDTPADQVTTTDLRTLSPGDSVDDAIQTMRQDD
                     VRRLPIVDGNRPVGIVSLGDLAIERDEDSVLADISSEPANN"
     gene            20070..23198
                     /locus_tag="VF752_14255"
     CDS             20070..23198
                     /locus_tag="VF752_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX8856756.1"
                     /translation="MRPFRGAALAAAVVLLSLCGSASAHPIATDDGSYAVLGRVFPDP
                     LAGCQVIGSAPCDPHAQGNVQADQFIQYDELINGLKYMNAKWGRNMEVWALDGKLGDG
                     SGTGLGADAFPGNNLGKLEFKPKKEYESAGLPTTDLGRKMADLIVVRVTDESVPDKGK
                     KRYALSLSIHGIERAGVEGGTRAMEDLVTAYSTGSTATDPSGRGDDPILPKEVAGNAP
                     TFGDVLKKTIIYFTYPNPDGWRRGSLDEGGVFFQRYNGNGVDVNRDWPDIGFSFRPYS
                     GLSEPESRAFSSFYTDVKRSTGAQFAAGDDLHGMPFADALSYTLLPHGRHDYGKDLRL
                     REAAKAIHRASENALSWSPIIQPNDAPKGGGVPCAPDVVVGEACAKIYGQTWGTVYDT
                     INYTTTGALGDWFDSSIGLGADGIDNEMAFSHLDKNVVFDKHAEQLHVDGNKALIWAH
                     LTEMLNPPKGSFDAPGAKGYVANTREQRPAKMYAPGPPKNTQPQEDITGVTGTPGADG
                     DIVFPFDVKQTAPAKGQPGIYNGGLRVDITQNNAQGIGNATATLKLQCKGCDRHRHAE
                     GQDDNDWVTVQEDYNQSPIYLQAGLTAAVNYPQALKEDGTPVEWRAVVSGGTLAARMD
                     VHFTSGPASTDGSTGGDPAPTTRAYDVANTDVFNDLNRFIPAAGDRFNAVDPRKVLGG
                     RQSLSGLKSLVLADNALPGYTGPYGGEAPAPTGPPTANKEFSSSASVPGGGSGAPGTF
                     EDYEFSTGANDGNGSMTVKVQWANGVNDFDLTLYRKNADGSLEELGSSAGGVPETSEE
                     VTLPNPPAATYVARVTNFAAPDPQWTGTLRFTAPASGPATGTGAYTPAEKDAWLAKLR
                     AFVVGGGNLVLTDGALRALPGLVSGMPDSAVARQTVYAGQTAFSTADGEDTLKDPLAR
                     DIAQDGARFSEGMRRQMFEPTPLGFSIQNQSTGSDESHARQFDIGRDAFEGSGGRVAG
                     TSADSGTRDAQPVYDRVTLGEIKLGKGTIRVAGALLPQPSEEFDHTLGLEPYAVTYTS
                     YIVLRNLLQPAA"
     gene            23245..23883
                     /locus_tag="VF752_14260"
     CDS             23245..23883
                     /locus_tag="VF752_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856757.1"
                     /translation="MAKQRQIKMQQRLGSLSAIPQLENRTDEQLLSESRNRAAARAIL
                     GARAAERYDAAAARKYFNEAMAGAHPQERPALRQMMKAALAQAERRPDELRDAMEKLG
                     QEPPSSRQMLMLRLMGLVAPPPNSSIFLRIRGWLLLVALVILVVAVGFGIVELISLPF
                     GGIGVAGGILLGLVAVAVVLGVLAFFGRRRQRAAVERRRGEMADAQRRLGRR"
     gene            complement(23887..>24068)
                     /locus_tag="VF752_14265"
     CDS             complement(23887..>24068)
                     /locus_tag="VF752_14265"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8856758.1"
                     /translation="WDEGEHERALALLEEALQSSENSERRDLIRRSMVGMFTELGPQS
                     EVASRYRRRLAAALN"
BASE COUNT         3541 a         8439 c         8289 g         3799 t
ORIGIN      
        1 cacaagtggc tgaacgtgcc gtacgactcg gggttcgtct tcgtgcgcga ctggagcgcc
       61 ctccagcgca ccttcggtga gcggggcgat tacctgccgc cgctcgacga cccaaacccg
      121 agcttcgcct acgtcgcacc ggagggctcg cgccgcgccc gcgcgctcgc agtgtgggcg
      181 acactgcgcg cctacggcgc ggacggctac cgcgggatgg tggagcgaca cgcccacctc
      241 gcgcgccacc tggcgcgact tgtggacgag caccccgcgc tcgagcgggt ggccgaggtg
      301 acgctcaaca tcgtctgctt ccgcgtgaag ccggagggcg tcccggagga gcggctcgac
      361 ggcctcaatc gtcgcgtcgg ggaagaactg ctggccgatg gccgcgtgtt cgccgggacg
      421 acgatctacg acggcaaggt ctgcttccgg ccggcgatcg tgaactggcg cacgcgggag
      481 agcgacgtgg agctgctgct cgatgtcgta ctcgagctga gcgagcgcct acaggcggcc
      541 gcggtctagt tcggtcgcgc ggtcagcgcg aacgtgccgg gtagcaagcc gcggaacgtc
      601 gcgacggccg ggttgacgcc ttccttcagc cggatcgtca aggtcacgtg cccgtccgcc
      661 tgggtgatcc cgctcttgcc gccggccgtc acgcgcgctc cctcgagcgg ccagcgcctc
      721 ctcccgaggc ggtagctcgc gcggaactcg accgtccgca gaccttcccc gatcacacgc
      781 ggaccggtga gcctgatcgc ataacggcca gccgggacca gttcgagcag cgcgccctgg
      841 ttgccaaagg tcggagggcc tgagtcgaat ggcaggatca tccagtggag caggccgccc
      901 atgtacgggc cgacatcgag gccctcggac tcgccgttga agcggcgctc gaactcgagc
      961 cggcgcttgc cggtcgtcag gtccacagac cagatctgaa acggtcccgc atcggatccg
     1021 gccacgaaga gcctgccgtc gtggaacacc gctcccgtga tcccgctcgg cggcacggcg
     1081 cgcttgagcc gcttcactgc cctgatcggg gcagccgcgg gagcggcgtt ggcggcggtc
     1141 acgtcggccg tgcgatacgc cagcaggtcc ttgcgcgccg aagtccacag cagcctgccg
     1201 tcgggcgagg gctctaccca catcgccttg gggatccccg ccgggtcgag cttcacgtag
     1261 tagcgccacg cgagcgtgct cgggtcggcc acggcgatgg agcccgtccc gcaggtgttg
     1321 cccccgttcg gcgctccagg cgtgtagcac tcgagcggga gcaggacgcg gccaccaccg
     1381 ctccggacga acccgatgtc gccaatgtgg ttgtaaccct cacgcaggcg gacgtcctgc
     1441 ggaatgatga gggtgttccc tctccgctgc gtcagcgcga gactgttccg gtagagcgcg
     1501 gcgtacgacg ccccgagcgc tccgtcgaag aacaggctcc ggccgttgga tgtgatgccc
     1561 tggttgtagc cgccggggaa gggatcgtgg cgaagcatcg cccagcggcc ggggtcgcgc
     1621 gcgagcgccg cgggcgcgac gagcgcgagc agaattggca cggccagcag cggtgcgagc
     1681 cgcttcaccc ggcgaaccct aggatgcccg catgacgctg gaacgcgtga ccgagagcga
     1741 gctgcccgaa cttctccccc tcatgcgcgc ctactgcgac ttctacgagg tgcagccgtc
     1801 cgacgacgcc ctgctcgcaa tgagccgcga gctgatcgcc gaccccgaga aggagggcgt
     1861 ccagctgctc gcgcgagacg acgacgggaa tgccgtcggc ttcgccacca tcttctggag
     1921 ctggtcgacc tcggacgctg cacgaatcgg agtgatgaac gacctgttcg tcgcggaggc
     1981 ggcgcgcggc ggcggcgccg ccgaagcgct catccgtgcg tgcgtcgacg agtgtcgcgc
     2041 ccgcggagct gggcgcctca cgtggcagac cgcgctggac aaccaccgcg cccaggctgt
     2101 ttacgaccga gtcggcggcg tccgcgagca ctgggtcgac tactggctcg aagcgcccgc
     2161 ctgagctccc gcggcctcgg ccccgttgcg atgcgtcagc gcgtaccgca ggttgacgcg
     2221 caccatgtgc gcgagctgag agggagccaa cagctccttc gggacgatgc gacggtcgcc
     2281 gtagcgccgc atgtgacgcg ccgcgtccag gtcgcgcgtg gcggccgaga acagcgcgcg
     2341 ctcgaacagg ttcatgcgcc ggccggtcga gtaggtcgat atggcgcggt ggtgggcggc
     2401 caggccggcg gtgtgccgct tgcgataccg cttcagcgcg tcgtcgaggt cgccctcggc
     2461 gtgtacggca tcggcggtgg cgtccacaag ccacgtggcc gactggaacg cgaagccgca
     2521 gcccactccc cacagcggat cggcggcaag cgccgcgtcg ccgatgaagg cgatagcggg
     2581 cgccgacgct cgtcgccgca ggttgggcat ttcgagcttt ccgatccaag gggtctcgcg
     2641 ctccgcgccg tccagccgcg gtccctcggg cagcgagtcg aacagacgcc gcaccgtccc
     2701 ttccatgtcg gccttgaact cagcgaggcg ctccttgtgc atgaagaggg cgaccgtcgt
     2761 gagctcgtcg tcagtgggaa acgcgtaggc gacgtctgga tccagcaggt acatctgggc
     2821 gacgccgggg cgacgcatct tcactccgct gaagttcgcg tagtagccga agcggttgtt
     2881 ggtcgacacc ttcgccggga tgcccgcgag ttcgccgacc ttcgagtcgc gcccgtcggc
     2941 gccgaccacg agccgggccg tgatctcgcg tgaggacccg tcacgtgact tggtgcgaac
     3001 tccggcgaac cgagctccgt gccggagcaa gccgtcggcg ctctcgccga gcagcaggcg
     3061 cactccctcg gtctcggatg cgatccggcg gagcagcggg tcgagcttcg cccggcgcag
     3121 gttgtaggcc cgcggctcgt catcctcagc tggaacgacc cagccccatt tcgtccagag
     3181 gtcgatgtgg gttcgcgtcg ccccctccgc ctccaggtcc gggaggatcc cgaggcgctc
     3241 gatgccctca gccgcgctcg gctggatgta gtgcgtgcag acgcgcttga accgtgcggg
     3301 gtccgtgtgg ctctcgatga gcgccacccg ggcgccagct cgcccataca ggatcgccgc
     3361 ggtacagccc gcgatgctcg caccgacgat tgcgacgtcg tattcgggga cgggggccat
     3421 gcgctccgtc caagctaacc gctccacccc ttttggggca agctcctcga tcagtccgtg
     3481 cgccaggcct gcgagcggcc gccggagcgc tttagcggtg tgttgcaaac ggcgccgcat
     3541 gcgggtagga caccgctggc tcgtggatga ggagcgcaac gacaccggtg gtgagccgaa
     3601 cgaatcggag aaggagcgga tcgaccgcga gctgatcgag ctgctcaacg agctgcgcgt
     3661 cgcgcttccc ggcgtgcagg tgctgttcgc cttcctgctg gcggtgccct ttcagcagcg
     3721 cttcgcgacc gtgaccgcgt tccagcgaga tgtgtactac ggcacccttc tcgccacgac
     3781 aatctcggcg gcgatgctga tcgcgccgag cgcgtaccac cggctgaact tccgcaggca
     3841 ggacaagcgg cgcctcctgg tgcactccaa ccggctcgcg attggtggcc tggcggcgct
     3901 tgccgtggcc atgtgctgcg tcgtgttgct gatcaccgac ttcctcttca gcacgaccct
     3961 cacgatcgtg tcgacggcgc tcgcgttcac gctgttcgcg gtcctgtggg tcgtcatgcc
     4021 gctgtggcgg aggcgttagc cggggcggtt gccgggcatc cgagccgcgc cgagcgattc
     4081 gcccttgcgg atgcgctcgc gctgcggccc cacggtgtac ttcggctccg aagccgactt
     4141 gaagcccgcg cggaagatgg cccagcgctc ggtgaaggca ccggccagca gcagccccgc
     4201 accgaccttc gcgagggtcc ggtcgctccg ccgccggcca cccttggcga gcaggcctgc
     4261 gcccgccgcg gtggtcgccg tcgcaagctt ggagagcgcg cccggaaggc ccttcttgta
     4321 gggctcgccc tcgaagcccg ctgcgtgctc cattccctgc gctgctccga tctcgagcgc
     4381 cgcgccgatc accgccagac gccgcgccgg ctcggcgtgc ttgccgggtg tcgctagcac
     4441 ggcggcggcg cccgcgctcg ccgcagcgcc gcccgcgaac acgaacggca ggaagtggcg
     4501 cgccttgtgc caggcgggga ccgccgtgtt gccgatcagc gctcccgtgt aggtagcgag
     4561 cggcatcccc gcgagcgcag ccacgagcct gccgctccgc gcgagccccg gaaagacgcc
     4621 ggtccacgcg tgcgccgcgg agacggtcac ggcgccgccg gtggtggcga ggatccacga
     4681 gcccacgctc atgggcgagg tgatcttgaa catcctcagc atgttgagga agcgcgcggg
     4741 cctgccgagg tcggagatga gcagcatggg gcttacgtgg atcccggcga gcgagatcgc
     4801 ccaggcgcgt cgcgagagct cgttgttgcc ctgcaggtga gccatgtacg cgaggcccgc
     4861 gctcgcgccc gccattcctc cgacgaagaa gtagatcggt atctcccacg tccagaccgg
     4921 ctcctggatg acgggctggt cgtagtagct gcgcggctca gcctccggga ccatcgcgcg
     4981 ctcgcccttg ccgttgcgcc gcggacgggt ttcgagcgtg gcgctcggca cctccggtcg
     5041 cgtgctcacc gccgccctcc gatgcacgcc gccgcgatgc ccacggcgag cgccaccgcc
     5101 gccgcgccgg tcgatgccca gatgccaggg agatcgcggg tggtgacgac ggggtccggc
     5161 ggcaggccgt acacctcggg ctcgtcgagc agcaggaaga acgccccgaa gccacccacg
     5221 ccgtcgtcgg gatgcgcgcc gtacagtcgc gcggatgtct ggcccgcgtc ctgcatctcc
     5281 tggagccgcg cctccgcgcg ctcgtgcagc tcagtcagct cgccgtactg gatggagttc
     5341 gtggggcatg cctgcgcgca ggccggctcc ttgtcgtcgc gcagccggtc gtagcagagc
     5401 gtgcacttcc acacgcggcc gtccggcttg cgcttcgaga gcactccgaa cgggcacgcg
     5461 ggaacgcagt agccgcagcc gttgcagatg tccgcctgca ccaccacggt gccgtactcg
     5521 gtgcggaaga gagcgccggt cgggcacacg tcgaggcagg cggcgtgcga gcagtgcttg
     5581 cagacgtcgg aagccatcag ccagcggagg ccctcgccct cctggagcgt gtcgagcccc
     5641 gagtcctcct cgagctcgcg acgggcggcg atctgcacga gtgactccgt ggcagtgtcg
     5701 ccgtcgagcg ccgccggcag ccgctgctcg atgaaagcga catgacgcca gttgtccgcg
     5761 tccagatgga gtgtgttgtc gtacgacttg ccggtgaagc ccttgaaatc ctccggcacg
     5821 tggttccact ccttgcacgc gacctcacac gccttgcagc cgatgcagat cgacgtgtcg
     5881 gtgaagaagc ccatgcgcgg cttcgccatc tcgccgtagg tgtccggcca ggcgccggcg
     5941 tcgagcgtga gcagcttggc gttggagctc actcgcccac caccttcact ccggcgcggc
     6001 gcttgtagtc ctcgaccagc cggagcacgt cggggccccg tggcctccgg ccgggaagga
     6061 tgtcacaggt ggcggccttc acctcctgga tgtgcacgtt cgggtcaaga acgagcggga
     6121 acaggtcgtt cgccgtgtcg cccttcacga tgccgccggg accccagtgg tagggcaagc
     6181 cgacctggtg gatcgtccga ccttcgactc gaagtggctt gacgcggtcc gtcacgttgg
     6241 cgcgtgcctc gatcgccgtc ctgctggtga cgatcgtggc ccagtcaccg tgctcgatgc
     6301 cacgctccgc ggcgagctcg gggctgatct cacagaacag ctcgggctgc agctgcgaga
     6361 ggatcggcac ggtgcggctc atcccgccgg cggtgtggtg ctcggtgagc cggtatgtgg
     6421 tgacgacgta ggggaagacg tcccagcccg gctcggggtc ggtggggttg taggggttct
     6481 cggggcggtt gaagcgctgc cgcagcgggt tctgctgctg cgtgtacagc gggttggtgg
     6541 ccggcgactc gtgcggctcg tagtgcgtgg gcatgggccc gtccatcaga cccgccggcg
     6601 cgtacagcca gccgcggcca tcggcctgga tgatgaaggg cgagtcgccg cgcagcgcgt
     6661 cgaggccgat gtcgttcttc ttcggctggt agtcgggcgg cttgtccgga acgaagtcgg
     6721 gatcgtcgta gccggtccac ttctgctgct cctcgtccca ccagacgtac ttcttgcgct
     6781 ccgaccaggg cttcccgctc gggtccgcgg aggcacggtt gtagatgatg tggcggttcg
     6841 ccggccaggc ccagccccac tcgggcgcaa cccagttctg ctcggtgtgc ggcttcttgc
     6901 gagccgcctg gttcaccccg cccttgtaga cgcccgcgtg gatccacgac ccgcagctcg
     6961 tggagccgtc gttcttcaag tcctggtact tctcgacgaa gctgccgtcg gcgtggcggc
     7021 cgccgatctc ctggagcacc tcttgggcgt ccggctcgtc gtgcggcccc tgcgtcgtgt
     7081 agttccacgt gaggttgagg agcgcgcggt cgcgcggctc tgtcgagccg gcgagcttgc
     7141 gccgcacgcg cctgccgagg tcgaaggtcc actgcagctc cgagcgtgcg tcgccctccg
     7201 gctcgaccgc cttccagtgc cactggagca gccgctgggt gttcgtgaac gagccatcct
     7261 tctcggtgtg cgaggcagcc ggcatgaaga agacctcggt gccgatctcc tccgtcttca
     7321 gctcgcctgt ctcgatctcg cggctgtcct tccagaacgt cgccgtctcg atcagctgca
     7381 cgtcgcgcac aacgagccat tcgaggtttg cgagcgcgaa gcgctgcatg cgcccgttgg
     7441 ccgagcccac caccgggttc tcgcccacga cgaagaagcc cgggcacttg ccgtcgagca
     7501 tcctgttgat cgccgcgtag tgagagtggt cggcgtcgat ccgcgggagc agcccgaagc
     7561 agaagtcgtt ctcccccgtt gcggcatcgc cgaaccaagc cttcaggagg ctgacggcgt
     7621 agttccgcat gtctccccag gcgcccgtcc tcggcgatgt gttcccgacc cacgtgtcga
     7681 ggtcggggcc cgcctgcggg tggggcatcg gaaggtaccc gggcaggatg ttgaacaggg
     7741 tcgggatgtc ggtcgacccc tggatgttcg cgtggccgcg cagcgcgagg atgccgccgc
     7801 ccgggcgccc gatgttgccc agcagcagct ggatgatccc ggcggtgcgg atgtactgaa
     7861 ccccgacggt gtgctgcgtc cagccgaccg cgtagcagaa ggccccggtc ctctcccggc
     7921 ccgagttctg gcagagcgtc tcggcgactt ggaggaacgt ctccttgggg attccgcaga
     7981 cctcctcgac gagctcgggc gtgtagcggc tgtagtgacg cttgaggatc tggaacacgc
     8041 agcgcgggtg ctgcatggtc tcgtcctctt tgggcggcct gccgcgatcg agcttcatgc
     8101 cgtgggcgcc gtgtgcctgc tcgccggaga cggcggcgcc ctgctccttg tgcccgccgg
     8161 cggtgtgttc ctcggtcccc gcgtacgacc aggtggatat gtcgtactgg ccggtctccg
     8221 gatcccaacc ggagaagaag ccgtcgaggt cctcggtgtc cctgaagccc tcatcgacga
     8281 tcgtggtcgc gttcgtgtag tgcttgacgt actcgtcgaa ccagcgctcg ttctcgatga
     8341 tgtagttgac gattccgccg aggaacgcga tatcggtgcc cgcccggatc ggcacgtgca
     8401 ggtccgccat cgcgctcgtg cgcgtgaacc gcgggtccac gtggatcacc ttcgcgccgc
     8461 gctgcttggc ctccatcacc cactggaacc cgaccgggtg gcactcggcc atgttcgagc
     8521 cctggatgac gatcacatcc gagttctgga ggtcctgctg gaaggtggtc gcgccgcctc
     8581 taccgaacga ggtgcccaga ccgggcaccg tggagctgtg tcatatgcgc gcctggttct
     8641 cgatctgaac ggcgcccaac gctgtgaaca gcttcttgat gaggtagttc tcctcactgt
     8701 cgagcgtcgc gcccccgagc caggcgatgc cgcgggtgcg gttgagcggg cggccttcgt
     8761 cgtcctccga ctcccacgtc ttctcgcgcg tcgcgatcac gcggtccgtg atcatctcca
     8821 tcgcctggtc gagcgggatc tcctgccaat ccgtggcgta cggcgcgcgg tagagcacct
     8881 tcttcgcgcg cgtggcgctg ttcacgtact gctcggaggc ggcgcccttc ggacacagac
     8941 ggccacgcga gatcgggctg tcggggtcgc cctcgatctg ggtgaccttg ccgtcctgca
     9001 cgtagacctt ctggccgcag ccgaccgcgc aataagggca gaccgactgg acgaccttgt
     9061 cggcggtctg cgtccggggc acgaggcgtg ccgagtgcct cgactgggcg gcgcggccca
     9121 gtccgagcgg gtccttcaac tgccgcagca gcggcggcag gtgatcggtc agctttccgt
     9181 tgccctcttc caacgcccgc cgcctcggta ccccgcggcg aatgggcata aacgagccgc
     9241 gcctgtcagc gccgcagcaa ccgtgcgacc acgttgcccg cgatcacgta cacgagtgcg
     9301 gcgaggcccc agttcaccac gatctcagtc ttgtgcttct cggggttgaa catgttgtgg
     9361 aacggctgag tgagccaact cgccgcatcg ttgatcgcgt tcacgatcgc gttgtccttg
     9421 ttcgcctcga gcaccacgaa ggcgatcccg atcacgatca ccagggcgac caggcccgcc
     9481 gtgagcgtga ccagcccggc cagcgtgttc agcgcgcgac gaccgccgcg gcccatcgcc
     9541 gcggtcatcg cggcagttct cccgggcgcg ccctcccggc ccgcgccgcc tcctgccagg
     9601 cgaagcctcc gcatctcccg cgaggctacc cgacgtttcc cgggcaggcg ccggttcgga
     9661 gacgcggttt gccgggtaat gcgccagcag accggcgcga ggaacaggag gacccacatg
     9721 gcggaaatca gtcaccagac agtcaggctg agccgcggca aacacgcttt cccggcgcaa
     9781 ggggtgtgcg tcatggagct cgcctcgatg ctggcgggcg aggacttctc ggatgcgccg
     9841 gagtgcgtct ccccggtcat cgggggcttc ctgcggacct acaacgacgg catcgacgac
     9901 cgcctgcgcc aggacctcta cgagttcgct gcgaaggccg tcgacactcg ctcgcgggcg
     9961 agcgtggagc agcgccgcgc gagtgactgc atcgcctggg cggcgtcgct cgccggatcg
    10021 ctgacgctgc gctttccggt gttcgcctgt acgacctcac tgaggggctg cgaggcggcc
    10081 ggcgcgtacg cggcgcgggc cgcgctgcgg cagggcccgg ctgcgcatgc cgaggcgctc
    10141 gagttcgtgg agcggctgat ggggctttgc gaggcggaca agcggccggc gccggctgct
    10201 gagcgccccg tcgccgcggt tgcgagctaa ctgggcggcg acatgaatgc gagcgcggtc
    10261 gccagcccgg ccgcgcgccg cacgctggcg atccgtcgcc ccggctgcac tgcgtcgtgc
    10321 ggctccccca ggagctcgcc gagactgcgc ctgcagcgat cgaggtcctc gaccgtcact
    10381 gtgagccccc agaacgaggc cgggtggtcg gcgtcgcggc cgtccgggcc caccacctcg
    10441 agcagcgcct cgccgagccg gtagaagccc tggccgatgg gctgaccgcc gggcgcggtc
    10501 gcttcgcgca cgcgccgcag gtcgagtccc gcgcgctcga gcgccgcgaa ggtccgatcg
    10561 aggtcggggg tcatcgccac gacgtggtcg atcgacaacg cgccgttggg atgccgaccc
    10621 ggagcgggca cgggatcgga gccattcgcg atcacggtgg gcagcccgtc gagctccgcc
    10681 tgctcgagcc cgcgaatcgc ccatcgcgcg atgccggatc ccgcgtcggg atcgaagacc
    10741 actcgcaccg agccgatctg cgcggcgtcg ccggcgaccg cgaagcccag cgcctcccag
    10801 cgctccggcg cgtccgcgat taccagctcc gccagatgcg gcgtattcac gcttgccagc
    10861 gtagtcctgc ggccagcccg ttgcgccccg ggcgtttcct gtacggcctt ggaggagggc
    10921 cttccccttc gggctccgtt cggcttcgcc tcactcttga cgccctccgg ggaagaccct
    10981 gcctcccggc ctactcgagc gtcgtcgttt gcgcgtggag cgtcgtcact caggggccat
    11041 agttcaccct gagcgacgac acctgccttt cgcgcgcacc agaagcgccg gcgccgggca
    11101 accctaagtg ctcaaacgag gaagacgcgc ggcagcaggc tcttgcgctc cctcctgcgc
    11161 gggagccgcg gccggaccga gctctccgtg ttcgagctgg aagcgcatgc ggcgcagcat
    11221 cgtcctcacg tcgacgcgct cggtgccgag cgcggcggcg gtcgcgcgcg agtcgaggct
    11281 cgtgtcgtag ggcacgggcg cgggcggaag ctgctcgggg tcgggcggac cgaagtcgag
    11341 cagctcgggg tcgagctcta actcctcggc cgtcagccgc gccagcgccg cgcgatcgac
    11401 cgactcgccg ccgcagcagt ggaacacccc ctctcttccg agctcggcca gtcgccacag
    11461 cgactcggcg gcgtcgctcg cgagcgtcgg cgtcgcgacg gtgttgatct cggggctctg
    11521 ccagaccgtg aatcgctgcc cggcgccgag cgcctcggcg atcgacgcca cgaggtagcc
    11581 gaagcccgcg tcctgacggc gcggcgtctc gggcctcgcc cagtgcacgc cgttcacgcc
    11641 ggagatgcgt gccacggcgc caccctcagc gcgctcggtc accaccagct cgctggcggc
    11701 cttcaggaag ccgtacaggt tgatcgggtt cggcggggtg tcctccgtgg cacccgactg
    11761 ggtgccgtcg aagacccagt cggtggagac gagcaccaca tgggcaccga ctgcgttcgc
    11821 ggcgtccacc aggttgcggg tggcgaccac gtacgacgcc cacgagtccc gccggtcggt
    11881 gtagaggcgc ctgaagtcgt tcaggatcgc cgcatgcacg atcacgtccg ggcgcgcgct
    11941 gcgcacgctg cccgcgacgg cgcttgcgtc cctgacgtcc gtgacggccc agtcgtacgg
    12001 cgccccggcg ggcggccgcg actcccgcac cgggcagaac agctcagccc cgtgccgctc
    12061 ggtgaacacg cgcgcgatgt tgctgcccac gaaaccggtc gcgcctgtga ggtatacgcg
    12121 cacggctatc ggtcgacctc gtccagcgcc acggggcgtc cttcctcgag cgaccgcttg
    12181 gccgcgagcc cgatcacgag aggcgcgcgc gcgtcggcgc cggtgaccgc cggggtttca
    12241 ccggtgcgaa cggcgccgac gaacgcctcc cactcgcgca cgtagctcgg cacgtagcga
    12301 tcgaggaaga agtagggaag cggcgcgccg tgcgccccgt ccgccgtgcg cacgacgccg
    12361 gtgtggccgg acgggttgtc ggaggccgcc atcccgcgcg agccgaatac ctcgacccgc
    12421 tgatcgaacc cgtagacggc ctggcggctg ttgtcgatcg cggttaggca gccgttgtcg
    12481 tgaacgagcg tcacgagcgc ggtgtccacg tcgccggcgt ccgcgatcgc cggatcgacg
    12541 cgcacggcgc cgtgggccga gacctgggtc acctcgctcg cggtcacgaa gcgtgccatg
    12601 tcgaagtcat ggatggtcat gtcgaggaag atgccgcccg aagcgcggat gtagtcgatc
    12661 ggcggcggcg cggggtcgcg actggaaatg cgcacgagat gcggatctcc cacgatcccg
    12721 ttcgcgaccg cgtcgcgcac cgactgatgt gccgggtcga agcggcggtt gaacccgatc
    12781 tggaacggga cgcccgcggc gtccaccgcg gcgagcgccc ggtcgacctc tgccagttcg
    12841 agcgagatcg gcttctcgca gaagatcggc ttgccggcgt tcgcggcggc gacgatgaag
    12901 tccgggtgcg tgccggtgct cgagcagatc gccacggcgt cgaggtcgcg cgagtcgagc
    12961 agctccgcca cgctcgcgca cgcacgcacg ccgaaatcgc ggcccgccgc gcgggaggcc
    13021 tcctcgtcgg cgtcgtgcac ggccgccagc tcggcgccgt cgacctgggt ggccaagagc
    13081 tccgcgtgca tgctcccgat ccgccccacg ccgatcacac cgatccgcag gggtgtctgg
    13141 gagtgctgcc gggaactcag cctgcgcgga ccggtccgcg tctgtccggc ggccggcatc
    13201 agtcctgcct cagcgagacg accggctgcc actgccccgt gcgcgcggac tcgagcagcg
    13261 ccgcctgcac gctcacccac tcgagcgcct cggcgaagcc gggccgatgt ggccgaccct
    13321 cggccaggga gcggcagaac tcgtagtcct cgatcacgat caggtcttcg aagccgatgc
    13381 cgttggcgct acccgggacg aagttgccgt ggtacgggaa gcggtcgccc ccgaagacag
    13441 tcgtgtagcc ggtgtggagc tcgccggacg cggcgtacac ctgcagctcg ttcagccgct
    13501 cctggttcca tcccacggcc ccgcccgtgc cgtagacgtc gaacgccatc tggctctcgg
    13561 gtccgacgat cgtcctgctc gcctcgaacg tgccgcgggc gccgttctcg aactcgcaca
    13621 gcatcgaggc gtagtcctcg ttggtcaccg cgccccgggg gtcctccggc cgtccgcgcc
    13681 cgtagtggct tgcgcccccc gcgccgggca gcggtcgctc cgggatgaac gtggccaccg
    13741 agccgcacac gcgcgtaacc ggcccgatca acatgtgcgc caggtcgacg gcgtggctca
    13801 gcaggtcgct ggtgactccg tggcccgcct cctccgccag gaaccgccac gacagcagcc
    13861 ccatggggtc gctgccatac atcgagaaga agcgaccgcg gtagttcgtg atctcgccga
    13921 ggcggccgga cgagatcagc tcggcggcat agcgcacgag cggcgcccag cggtagttgt
    13981 agcccacgcc ggtgttcacg ccggcgtgcc tggcaatgcg ctcggcccgc gctgtctgct
    14041 cgggcgtgcc gccgaccggc ttctcgcaga agacgtgctt gcccgcctgg gcggccgcct
    14101 cgatcagctc gaggtggagc gagttcggcg cggcgatgaa caccgcctcg acgtcggggg
    14161 tgtcgaccac ccggcgccag tccgccacac cctcgcggta gccgaaggag ccgaccgcct
    14221 cctcgagccg cgccggcacg gtgtcgctgc agacgacgag ctccgggtcg aactcacgct
    14281 cggggaagag cgtgcgcacg cgctgcagcg agcgactgtg ggcctggccg agccatccga
    14341 acccgatcag gcccacgccg atccgcctgc gtgcccggcc aggcgggccg acccggcccg
    14401 ccccggcctg gctgcgcgtc tcctcgggag cgtgcgtcac tgggtcgtct gaacttcggg
    14461 cgcgcgctcg agctcatgcg tgagctggtc gagctctgct cctccggcca tcatccgcac
    14521 gagctcgtcg cgactgatct cgccgcgctt ccacgagccc tgaaggtgtc cgcgattgag
    14581 gatgacgaac cggtcgccca ccgggtacgc gtggtgcggg ttgtggctga tgaagatcac
    14641 ccccacaccc cggtctcgcg cctgcgcgat gtagcggagc acgatccccg actgcttcac
    14701 accgagcgcc gaggtcggct cgtcgaggat cagcaccttg gcgccgaagt agatcgcgcg
    14761 cgagatcgcc acggcctgcc gctcgccacc ggacagcgtg cccaccggct gatccgggtc
    14821 acgcacgtcg atgcccatct tcttgagctc ctcacgcatg atccgcttcg cgctgttcac
    14881 gtccaggaac ccgaacggcc cgatcttctt ctcgggctcc gagccgagga agaagttccg
    14941 ccagatcgac atgagcggga cggtcgcaag gtcttggaag acggtcgcga tgccgcgctc
    15001 gcgcgcatcg cgaggcgagg cgaagctcac cttgtccccg ttcatgtaga gctcgccctc
    15061 gtcctgcttg tggactccgg agaggatctt gatgaacgtg gacttgccgg ccccgttgtc
    15121 gccgagcacg caggtgacct cgcctgcccc caccgcggcg gatacgtcct tgatcgcgat
    15181 cacgttgccg aagtacttcg agacgccccg cacctcgagg agcgcgtggt tgccgtccgt
    15241 ctgagttgcg gtcatctcct cgctccttcc gcgctgcgac gcacgaagtt gttgatcagc
    15301 acggcgagca ggaggatcac gccgaggaac aggaagcgcc agtcggtgtt ccagccctcg
    15361 aactggatgc cgatgaacgc catcccgatg atcagcgcac cgatcgccgc tccgaacgcc
    15421 gacccgaagc cgccggtgag caggcagcct cccaccaccg ccgcgatgat gtagttgaac
    15481 tcgtcgccga tgccttgccc cgcctgtacc gatgcgagac ggagggcgat catgatgccg
    15541 acgagcgccg aagcggtcgc cgtggtcatg aaaagggcga tcttcacgcg cgagaccggg
    15601 acgcccacgt tacgcgcggc aacctggtcg ccacccacgc cgaagatcca gttcccaacg
    15661 cgagtgcgag cgaggaccca cgtggcgatg gcggtgatgg cgatccacca cagcaccgtc
    15721 acgcggaagt cgtgcggcga ccagaacgag ccgcctagca ccttgaaggc gctgtcatag
    15781 ccgctcacgt cgccgatccc ggccacgcga acggtgcccg tgatcgcctt ggtcacgccg
    15841 aggttcacgc cctgtaggac gaagaacgtg gccagcgtga cgatgaagct cgggagcttc
    15901 gtcctcacca ccatgtagcc gttgatgaag ccgaccatgg cggcagccac gagcgtcagc
    15961 aggatcgccg gccagatgct catgccgacc tcggtggcga ggatgcccat gaacaggccc
    16021 gagctgccgg tcatgacgcc ggcggacaga tcgaactcgc cgccgatcat gagcagcgcc
    16081 acagcgacgg ccgggatgcc gagcgtggac gcaacgtcgg tccagttcgc ggcgccgtcg
    16141 agcgtgccga actcgctcgt ggttacgcca aaaaagattc cgatgaccgt cgcggccatc
    16201 agcgcgccca gttcggggcg tcgcagcagc ttcgtgagca ggctgacgtg cgccagtcgc
    16261 tcgtcgacga cgcttaccgg cgccggcggc gttggtgccg gaaccgttgt cgcttggtcg
    16321 ctcatcccct caatccctcc taactgccgg aggccggccc gcagcagcga gccggcctcc
    16381 gtatgcggtt agcgcgtgcc ttctcccgcc agcttctcga cggtgccggc gttggacttg
    16441 tccacgaatc cggggccggt gagaactggc ttgccgcccc cgacggtgtt gttgttggtc
    16501 ttgtagagcg tcaggaacac gactggcagg tagccctgtt cgtactgctg ctggtcgacc
    16561 gcgaactcga tctcgccgct cttgatcgcc ttcacgacgt caccgctcag gtcgaacgtg
    16621 gcgagcttcg ccttcgagtt cgcgcccttg atcgcgtcgc gcgcggcgac cgcgatgtcc
    16681 gggttcaggg tcaggaccga gtcgatcgag ctgtccgagc ggagcttgga ctggatctcg
    16741 gtcaggctcg tctggatgtc gttgacgccc ttgacctgga cgttctcgac ggtgccgccg
    16801 aagcccttct tggctccgtc gcagcgctgg ttcaagccga tgttgttctg ctcgtggatc
    16861 acgcagagca gcttcttgcc gccggcgtcc gcgagccgct tgccggccgc ctcgccggcg
    16921 atgctctcgt cctggccgac gtgggtgatg gcgcccagct tcgccgagtc gttctgaccc
    16981 gagttcagcg tgatcacggg aatgcccgcg tcctcggcct tcttcagcgg atctgcgatc
    17041 gcgtccgggt tcggggccga cacggcgatg ccgtccacct tctgcgtgac agccgcgtcg
    17101 atcagctgcg cctgcttctg cgggtcgttc tgcgactcgc tgtagttgac cttcacgccg
    17161 aggtccttgc ccgcctgctc ggcgcccttc ttggccaccg cccagaaggt gtcaccctcg
    17221 ccgtgcgtga tcacggcgaa cgtgaggttc tttccctgcg tggccgacac gttgtcaccg
    17281 gtcgacccgt tgttgtcgtt accaccgccg cagcccaccg cgaacgccgc aaccgccagc
    17341 gccaggacca ggaccgcgac ggggccaaag agacgtctca tccgacatcc tccttaactg
    17401 gagccagcat gtgaacgcag ccggcgtacg actggacgcc ggtcagcggt ccgccggttg
    17461 ccgggggctc cgtcccggtt tggcggtgtc gtgccgagga cgagcgccga cgatcgtgcg
    17521 gccgtggccg cggcgccgga acagttggtg gtgaccctct ccccatcccc gggttagagc
    17581 gctgcaattg ttagagcgct ctaacgcggg tagtcttatc cgcgcggacc atggctgtca
    17641 aggagtcgca aggaagcctt aaccgaagcc gcccggcgca tccgaccatg gacgatgtcg
    17701 cccgcaaggc cggcgtctcg cgggcgctcg tgtcgctcgt catgcgcgag agcccgaagg
    17761 tgagccccga gcggcgcggc cgagtactcg cggccgcctc ggagctcggc taccgcccaa
    17821 acgccatggc gcgcagcctc gcgagtcgca ggactcgcac cgtcggcgtg ctcctgaacg
    17881 acctgcacaa ccccttcttc gccgagatcg ccgacggtat cgaagagctt gcctcggagc
    17941 tgggctaccg cctgctgatc acgagcggcg ggcgccggca acgccgagag cagacgatgc
    18001 tcgagggcct gctcgagttc cgcacggatg ggctgatcct cgtgagcccg cggatgcgca
    18061 ccgccgagat gctcgcggcc accggcggcg cgcccgtcgt cgtcgtgagc cgcgtggttc
    18121 gaagcgactc ggtcgactgc gtcaccacgg acgagtcact gggggcgcgc cttgcggtgc
    18181 ggcatctcgt ggagctgggt cacaagcgga tcgtgcacgt ggacggcggc cagggcgcgg
    18241 gctcggcacc tcgccgcggc ggctacctgc gcgcgatgcg tgaggtgggc ctcgagcagt
    18301 acgcccaggt gatagcgggc gacttcaccg acgcggccgg cgtgcgtgct gcggagcagc
    18361 ttctcgagtc gcccgagctg ccgacggcgg tcttcgccgc caatgacctg gtcgccgcgg
    18421 gtgtgctcga ccgtctcgag gacgccggcg tgagcgtgcc cggcgacgtc tcgatcgtcg
    18481 gcttcgacaa cacgttcctc gccgcgctcc accacatgtc gctcacaacg atcgaccagc
    18541 cgcgccccga gatgggccgg ctcgcgctcg agctgctgct cgagcggatg gacgggcgtg
    18601 agccgccggc gatgcgagtg gtcgagccga cgctcgtggt gcgcagtacc tcgggacccc
    18661 cgagatgagc gccctaaggc tcggcgcgct ggcctgcctg gcgctcgtcg cagccgcggt
    18721 cgcgatcgtc gccacgaggt cgtcgacgcc ccagcacggt ccccagagct cgcccacggt
    18781 ggtcaccgtg ctcgcgcgca gcggcacggc ggccgaggag aaggccaccg gattcgcagt
    18841 tgcgaacgag cgggtggtga cggtcgccca cgccctgagc ggctccgccg ttgccgaggt
    18901 gctcgatcag cgcggcgtcg tgcatcgcgg cagcgtggtc cgcagggatg cccggctcga
    18961 tctcgcgctc ctcagcgtgc ccggcctgcg ggctggcgag atgcagcccg ctgcctccac
    19021 cgggcgcgct cgcgtgctcg tgatgagagg gagcgacgtg gcgcgcctgc ccgccgacat
    19081 tcgccgtcac gtggcattcc acctcgtcga ccgtgtggcc gcccgtacgt acgaccggcc
    19141 cgctctcgag ctcgccgccc gtatcgacgc cggcgactcg ggcgcgcccg ttctcgccgg
    19201 cgggcgcgtg gccggagtgg tgttcggccg gtcgaacatc cgggacggca cggcctacgc
    19261 cgtcgacgcc gaagccctga acaggctagt gagcccgcgc tgaacggacc cgcaaagagt
    19321 tgagccgccg cacggggcga atgtgcggcg gctcgtatgg ggcgtcaacc aggtcggcag
    19381 cgtgagggcc gccgaggccg gtgatgcctc aacttcagtt gtttgccggc tccgagctga
    19441 tgtccgcgag gaccgagtcc tcgtcgcgct cgatcgcgag gtcgccgagt gaaacgatcc
    19501 ccaccggccg gttgccgtcg acgatcggaa ggcggcggac gtcgtcctgc cgcatcgtct
    19561 ggatcgcgtc gtccacgctg tcgcccggcg acagcgtgcg gagatccgtc gtggtgacct
    19621 gatctgccgg cgtatccggg tcgaggccgt cggccacggc gcgcacggcg atatcgcggt
    19681 ctgtggcgat gccgaagagc cgcccgttgt cggtcacgag gatggcgccg gtgtcggcat
    19741 cccgcatctg ccgggccacc tctgcgatcg gcgtgtcacg ctcgaccgtg acgggatcgc
    19801 ccgtcatgat ctcgctgatc ttctgatcgc gctggattcg cgccaagaac ctgcctcctg
    19861 ccctatcgat ccctctccat ggaggcggcg gagccggacg aggcttcccg caggccttgt
    19921 ccggcgcgcc tcctgtcctt gggattgccg gggtccgaag gcggtaaacc tgaccatcga
    19981 ttcggggcct ctcaaatgcg ccaatcggcg cgcgtcgtcc cccacgttgg taaaaatgcc
    20041 gccgtctact agcaggggag ctaggcaaaa tgcgtccatt ccgcggtgcc gcactggcgg
    20101 cggcagtcgt tctgttgagt ctttgcggga gcgcgtcggc gcacccgatc gccaccgacg
    20161 acggctcgta tgccgtgctc gggcgagtgt tcccggaccc gctggcggga tgccaggtca
    20221 tcggcagcgc gccgtgcgat ccgcatgcgc agggcaacgt gcaggcggac cagttcatcc
    20281 agtacgacga gctgatcaac gggctcaagt acatgaatgc gaagtggggc cggaacatgg
    20341 aggtctgggc cctcgacggc aagctgggcg acgggtccgg caccggcctt ggcgccgacg
    20401 ccttccccgg taacaacctc ggcaagctcg agttcaagcc gaagaaggag tacgagtccg
    20461 ccggcctgcc gacgaccgac ctcgggcgca agatggccga cctgattgtg gtgcgggtga
    20521 ccgacgagag cgtccccgac aagggcaaga agcgctacgc gctgtcgctg tcgatccacg
    20581 gcatcgagcg cgccggcgtg gagggcggca cgcgcgcgat ggaggacctc gtgaccgcct
    20641 acagcacggg ctcgactgct accgacccgt ccggccgcgg cgacgacccg atcctgccca
    20701 aggaggtggc gggcaacgcg ccgacgttcg gcgacgtgct gaagaagacg atcatctact
    20761 tcacctatcc gaacccggac ggctggcggc gcggctcgct cgacgagggc ggtgtcttct
    20821 tccagcgcta caacggcaac ggcgtggacg tgaaccgcga ctggccggac atcggcttct
    20881 cgttccggcc ctacagcggc ctgtcggagc ccgagagccg cgcgttctca agcttctaca
    20941 ccgacgtgaa gcgctcgacc ggcgcccagt tcgcggccgg cgacgacctc cacggcatgc
    21001 cgttcgcgga cgcgctgtcc tacacgctgc tgccgcacgg acgccacgac tacggcaagg
    21061 acctgcggct gcgcgaggcc gccaaggcga tccaccgcgc gtccgagaac gcgctcagct
    21121 ggtcgccgat catccagccg aacgacgccc cgaagggcgg cggtgtccca tgtgcgcccg
    21181 acgtggtggt cggcgaggcc tgcgccaaga tctacggcca aacctggggc acggtttacg
    21241 acaccatcaa ctacacgacc accggcgcgc tcggcgactg gttcgactcg tctatcggcc
    21301 tcggggcgga cggcatcgac aacgagatgg cgttctccca cctcgacaag aacgtggtgt
    21361 tcgacaagca cgccgagcag ctccacgttg atggcaacaa agcgctgatc tgggcgcacc
    21421 tcaccgagat gctcaacccg ccgaagggct cgttcgacgc gcccggcgcg aagggatacg
    21481 tggcgaacac ccgcgagcag cgcccggcga agatgtatgc ccccgggccg cccaagaaca
    21541 cgcagccgca ggaggacatc accggcgtta ccggcacacc cggcgccgac ggcgacatcg
    21601 tgttcccgtt cgacgtcaag cagaccgcgc cggccaaggg ccagccgggc atctacaacg
    21661 gtggcctgcg cgtggacatc acccagaaca atgcccaggg cattggcaac gcgacggcca
    21721 cgctcaagct gcagtgcaag ggctgcgacc gccaccgcca cgccgagggc caggacgaca
    21781 acgactgggt cacggtgcag gaggactaca accagtcgcc gatctacctg caggcgggtc
    21841 tcacagcggc ggtgaactac ccgcaggcgc tgaaggagga cggaacgcca gtcgagtggc
    21901 gcgcggtcgt gtcgggcggc acgctcgccg cccggatgga cgtgcacttc acctccgggc
    21961 cggcctcgac cgacggctcg accggcggcg atccggcgcc caccacgcgc gcctacgacg
    22021 tcgccaacac cgacgtcttc aacgacctga accgcttcat tccggccgcc ggcgatcgct
    22081 tcaacgcggt cgacccgcgg aaggtgctcg gcggtcggca gtcgctgtct ggcctgaagt
    22141 cgctcgtgct tgccgacaac gcgctgccgg gctacaccgg tccctacggc ggcgaggcgc
    22201 cggcgcccac cggcccaccg actgccaaca aggagttcag cagctcggcg agtgttcccg
    22261 gcggcgggag cggcgccccc ggaacgttcg aggattacga gttctcgacc ggggcgaatg
    22321 acggcaacgg gtcgatgacg gtgaaggtcc agtgggccaa cggcgtgaac gacttcgacc
    22381 tgacgctgta ccggaagaac gctgacgggt cgctcgagga gctcggctcc tcggcaggcg
    22441 gagtgccgga gacctcggag gaggtcacgc tcccgaaccc gcccgccgca acgtacgtcg
    22501 cccgcgtcac gaacttcgcc gctccggacc cgcagtggac cggcacgctg aggttcacgg
    22561 cgccggcgtc gggcccggcg accggcacgg gtgcctacac gcccgcggag aaggacgcgt
    22621 ggctcgcgaa gctgcgcgcg ttcgtcgtgg gcggcggcaa cctcgtgctc accgacgggg
    22681 cgctgcgggc gctgccgggc ctcgtgagcg gaatgccgga ttcggctgtc gcgcgccaga
    22741 cggtttacgc cggtcagacg gcgttctcga ccgccgacgg cgaggacacg ctgaaggatc
    22801 cgctcgcgcg cgacatcgcc caggatggcg cccgcttcag cgaggggatg cggcgccaga
    22861 tgttcgagcc cacgccgctc ggattctcga tccagaacca gtcgaccggc tccgacgagt
    22921 cacacgcgcg gcagttcgac atcgggcgtg acgcgttcga gggttctggt ggccgcgtgg
    22981 cgggcacctc ggccgactcc ggtacgcggg atgcccagcc ggtgtacgac cgcgtgacgc
    23041 tcggcgagat caagctcggc aagggcacga tccgcgtggc gggcgcgctg ctcccgcagc
    23101 cgagcgagga gttcgaccat acgctcgggc tcgagcccta cgcggtcacg tacacgagct
    23161 acatcgtgct gcgaaacctg cttcagcccg ccgcctgagc gggtaggtca taggggccgg
    23221 ggtcggctct agactccggc ccctatggcg aagcaacggc agatcaagat gcagcagcgg
    23281 ctcgggagcc tgtcggcgat cccgcagctg gagaaccgca cggacgagca gctcctatcc
    23341 gagtcgcgca accgcgccgc ggcgcgagcc atcctcggcg cccgggcggc cgagcgctac
    23401 gacgcggctg ccgcgcgcaa gtacttcaac gaggccatgg ccggcgcgca cccgcaggag
    23461 cgtccggcgc ttcgccagat gatgaaggcc gcgctcgccc aggctgagcg ccgccccgac
    23521 gagcttcgcg acgcgatgga gaagctcggc caggaaccgc cctcgagccg ccagatgctg
    23581 atgctgcgcc tgatgggcct cgtcgcgcct ccgccgaact cgagcatctt cctgcgcatc
    23641 cgcggctggt tgctcctcgt ggcgctcgtg attctcgtgg tcgccgtggg cttcggcatc
    23701 gtcgaattga tctcgctgcc gttcggcggc atcggcgttg ccggcgggat cctgctcggg
    23761 ctcgtggcgg tcgccgtggt cctcggcgtg ctcgccttct tcggacgacg gcgccagcgc
    23821 gccgcggtcg aacgcagacg cggcgagatg gccgatgctc agcggcggct cggcaggcgc
    23881 tgagcgctag ttgagcgccg ccgccagccg gcggcgataa cggctcgcga cctcgctctg
    23941 aggcccgagc tccgtgaaca tcccgaccat cgagcggcgg atcaggtcgc gccgctcgga
    24001 gttctccgag gactggaggg cttcctcgag aagcgccagc gcccgctcgt gctcgccttc
    24061 atcccacg
//
LOCUS       DASVXS010000250         5089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_565151, whole genome shotgun sequence.
ACCESSION   DASVXS010000250 DASVXS010000000
VERSION     DASVXS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565151"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1596)
                     /locus_tag="VF752_14270"
     CDS             complement(127..1596)
                     /locus_tag="VF752_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8856759.1"
                     /translation="MTTTDTTAADSLIDELRAACGDANVVTHPHQMRTYESDGLLRYR
                     VTPRAVVLPGSAEEVQAVLRASHDAQVPWVARGSGTGLSGGALPVEEGIVISVNRMNR
                     VLEVDLANQRVVLEPGVTNLQVSKAVGPTHFYPPDPSSQIVCSIGGNVAENAGGAHCF
                     KYGFTTNYVTGLELVLPDGELIQLGGKELDRPGYDLLGAFVGSEGTLAVATKITVRVI
                     PKPEAVRTLVAFFDDTSHAGRVVSDVVAAGIVPGAIEMMDSLAIEAAEGATHAGYPPD
                     AGAALVVELDGSEAECDHRFEEVKEICQRNEATGIRVAQDETERELIWKARKAAFAAM
                     GRVSTHYYVQDGVIPRTRLPEVLARIDELSREYGMPVANVFHAGDGNLHPLVCYDGSK
                     EGEAERAEELSGEILAACVEAGGSITGEHGVGFDKKGYMPRMFDEPDFDAFQRLRCSF
                     DPAGLANPGKVMPTPRLCGEVPGPYRQHPLEAAGIAERF"
     gene            complement(1624..3294)
                     /locus_tag="VF752_14275"
     CDS             complement(1624..3294)
                     /locus_tag="VF752_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX8856760.1"
                     /translation="MYKQVLDPVGNALGWSSLFAALPLIVLFVLLGGIRMKAQWAALA
                     ALLTAILVALIAYDMPFGQTMDSAAEGAVFGFFPIMWIVINAIWVYNMTRETGHFAVL
                     RRSFATVSADERIQAVIIAFSFGALIEALAGFGTPVAITSVMLIALGFRPIKAAAVAL
                     VANTAPVAFGAIAIPIVTLAEVSGLPKDDLGAMVGRQTPFLALIVPLILIAMVDGRRG
                     LRQAWPAAVVGGVTFGALQFATSNYISVELTDIIASLGSAAAIVMLLQVWQPSEPLLA
                     EEPAAPGRPAIAGAESYNPTLEAEVKRRDDETKDSRRDIILAYAPYAIIIAVFGIAQI
                     GVIKDWLDGLGNEFKWPGLDIVNAKGKEPMSVTFKFNWANAAGSLMLVAGILTMLVLR
                     FSPMRALRTYWATLVQLRWAIVTVMSVLALAYVMNQSGQTITLGTWAAEAGAFFAFLS
                     PLIGWFGVAVTGSDTSSNALFGALQVTAAKDAGLSQTLLAAGNSSGGVLGKMISPQNL
                     AIGAAAVGMSGREGDLFRRVLLWSLVLILIMCVLVFLQSKPVLDWMVV"
     gene            3355..4053
                     /locus_tag="VF752_14280"
     CDS             3355..4053
                     /locus_tag="VF752_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX8856761.1"
                     /translation="MQVSLFITCLADTLFPETGRALVRLLERLGHEVEFRPEQTCCGQ
                     MHFNTGYRDEGLALARRLERVFDGAEAVVSPSASCVGMVRENCSPELGSRVFELSEFL
                     VTRLGVDDVGAYYPHRVTYHPTCHSLRVLRVGDAPLRLLRAVRGIDLAELEQAAECCG
                     FGGTFALKNADVSSAMLADKIGHVLETRAEVCAAADTSCLMHIGGGLARQRAGVRTVH
                     LAEILASTEEEPLR"
     gene            4050..>5089
                     /locus_tag="VF752_14285"
     CDS             4050..>5089
                     /locus_tag="VF752_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEX8856762.1"
                     /translation="MTSFPDAARQALGDSQLRHNLGNATRSIREKRARAVGELPDWEE
                     LRTAGAAIKEHTLRRLDSYLVALENAVQARGGVVHWARDGAEACSIVAALARDAGASE
                     VVKVKSLTTDEIELNKALEAAGIDAVETDLAELIVQLDGDWPSHILVPAIHRNRAEIR
                     NIFRTHLDGVEALSDEPSALTEAARLHLRRKFLAAGVAVSGANFAVAETGTVCVVESE
                     GNGRMCLTLPEVLITVMGIEKVVPSWRDMEVFLQLLPRSSTAERMNPYTSCWSGVSAG
                     DGPQEFHLVLLDNGRTEVLRDSVGRQTLRCIRCSACLNVCPVYERVGGHAYGSIYPGP
                     IGAILTPQLRGV"
BASE COUNT          799 a         1777 c         1711 g          802 t
ORIGIN      
        1 cccagccgag cttggtgccg cctccgctca cgcgcagacg caggccatcc cggccgcagt
       61 cggccaccag cgccgcggcc tcgtcctggc tcgccggggc ctcgctgcgt agctcgcgcg
      121 tggccatcag aagcgctccg cgattccggc ggcttcgagc gggtgctgcc ggtacggccc
      181 gggcacctca ccgcacaggc gcggggtggg catcaccttg cccgggttcg ccagccccgc
      241 cgggtcgaac gagcagcgca gtcgctggaa ggcgtcgaag tccggctcat cgaacatccg
      301 cggcatgtag cccttcttgt cgaagcccac gccgtgctcc ccggtgatcg atccgcccgc
      361 ctcgacgcac gcggccagga tctcgccgga cagctcctcc gcgcgctcgg cctcaccctc
      421 cttcgacccg tcgtagcaga cgagcgggtg cagattgccg tcgcccgcgt ggaacacgtt
      481 cgcgaccggc atgccgtact cccgcgaaag ctcgtcgatg cgcgcgagca cctcgggcag
      541 gcgcgtgcgt gggatcacgc cgtcctggac gtagtagtgc gtggaaacgc gacccatcgc
      601 ggcgaacgcc gccttgcgcg ccttccagat cagctcgcgc tcggtctcgt cctgtgcgac
      661 ccgtatgccg gtcgcctcgt tgcgctggca gatctccttg acctcctcga agcggtggtc
      721 gcactcggcc tcggacccat ccagctcgac caccagggcc gcgcctgcgt ccggcggata
      781 gccggcgtgg gtggcgccct ccgccgcctc gatcgcgagc gagtccatca tctcgatcgc
      841 gcccggcacg atccccgctg ccaccacgtc cgagacgaca cgcccggcgt ggctcgtgtc
      901 gtcgaagaac gcgacgagcg tgcgcaccgc ctcgggcttc gggatcactc gcacggtgat
      961 tttcgtggcg actgcgagcg tgccttcgga gcccacgaag gcgccgagca ggtcgtagcc
     1021 ggggcgatcg agctccttgc cgccgagctg gatgagctcg ccgtcgggca gcactagctc
     1081 gaggccggtc acgtagttcg tggtgaagcc gtacttgaag cagtgcgcgc cgccggcgtt
     1141 ctcggccacg ttcccgccga tcgagcacac gatctggctc gagggatcgg ggggatagaa
     1201 gtgagtcgga ccgacggcct tcgagacctg caggttcgtt acgcccggct cgagcaccac
     1261 gcgctgattc gccaggtcga cttcgagcac gcggttcatg cggttcaccg agatcacgat
     1321 gccctcctcc acggggaggg caccgccaga gagacccgta ccggacccgc gcgccaccca
     1381 cggcacctgc gcgtcgtggc tggcgcgcaa taccgcctgg acctcctcgg ccgagccggg
     1441 caggacgacg gcgcgcggcg tcacacggta tcggagcagt ccatccgact cgtaggtccg
     1501 catctggtgc gggtgcgtga caacgttcgc gtctccgcag gccgcgcgca gctcgtcgat
     1561 caggctgtcg gcggcggtgg tgtctgtagt ggtcatcgcc gccaagtatg ggcctccgct
     1621 ctcctagacg accatccagt ccagcacggg tttcgactgc aggaacacga gcacgcacat
     1681 gatcaggatc agcacgaggc tccagagcag cacgcgccgg aacaggtctc cctcgcggcc
     1741 gctcatgccg accgctgccg ctccgatcgc caggttctga ggcgagatca tctttccgag
     1801 gaccccgcct gaactgttcc cggctgcaag cagcgtctgc gacaggccgg cgtccttcgc
     1861 cgcggtcacc tgaagcgcgc cgaacaaggc gttcgacgag gtgtccgagc ccgtcactgc
     1921 cactccgaac cagccgatca acggcgacag gaacgcgaag aaggcacctg cctcagccgc
     1981 ccacgtgccg agcgtgatcg tctggccaga ctggttcatc acgtaggcga gcgcgagcac
     2041 cgacatgacg gtcacgatcg cccagcggag ctgtaccagc gtggcccagt acgtgcgcag
     2101 cgctcgcatc gggctgaagc gcagtaccag catcgtgagg atgccggcca ccagcatgag
     2161 gctgcctgcg gcgttcgccc agttgaactt gaacgtgacc gacatgggct ccttgccctt
     2221 tgcgttgacg atgtccagcc ctggccactt gaactcgttc cccaagccgt cgagccaatc
     2281 cttgatgacc ccgatctgcg cgatgccgaa gaccgcgatg atgatcgcgt acggcgcgta
     2341 ggcgaggatg atgtcgcgac gggagtcctt cgtctcgtcg tcgcgccgct tgacctcggc
     2401 ctcgagcgtc gggttgtagc tctccgcgcc ggcgatcgcg ggccgaccag gtgctgccgg
     2461 ctcctccgcc agcagtggtt cggacggctg ccagacctgc aaaagcatga cgatcgcggc
     2521 agccgagccg agcgaggcga tgatgtcggt cagctcgacg gagatgtagt tcgacgtggc
     2581 gaattgcagt gcgccgaagg tgacacctcc gaccaccgcg gccggccagg cctggcgaag
     2641 gccgcgccgt ccgtcgacca tcgcgatcag gatcaggggc acgatcagcg cgaggaacgg
     2701 cgtctgccgg cccaccatcg cgccgaggtc gtccttgggc aggccgctca cctcggcgag
     2761 cgtcacgatc gggattgcga tcgcgccgaa ggccaccggc gccgtgttgg cgacgagtgc
     2821 cactgcggca gccttgatcg ggcggaatcc gagcgcgatg agcatgacgc tcgtgatcgc
     2881 gaccggcgtg ccgaacccgg ccagcgcctc gatgagtgcg ccgaagctga atgcgatgat
     2941 caccgcctgt atccgctcgt cggcgctcac cgtggcgaac gaccggcgga gcactgcgaa
     3001 atgccccgtt tcgcgggtca tgttgtagac ccagatcgcg ttgatgacga tccacatgat
     3061 cgggaagaag ccgaaaacgg ccccctccgc cgcggagtcc atcgtctgcc cgaagggcat
     3121 gtcgtacgcg atcagcgcca ccaggatcgc cgtcagcagc gccgccagcg ccgcccactg
     3181 agccttcatt cggatgccgc cgagcagcac gaacagcacg atcaacggca gcgcagcgaa
     3241 cagcgacgac catccgagcg cgttccctac cggatccagc acctgcttat acattccgtc
     3301 tcctcctccc tgatgcacga gtgcccgcgc ctgaagaggg aaaaacgcct ttagatgcag
     3361 gtctcgctct tcatcacctg cctggcggac acgcttttcc cggagaccgg gcgcgcattg
     3421 gtgcgtctgc tagagcggct cgggcacgag gtggagttcc ggccggagca gacgtgctgc
     3481 gggcagatgc acttcaacac gggctatcgc gacgaagggc tcgcgctcgc gcggcgcctc
     3541 gagcgcgtgt tcgacggcgc ggaggcggtg gtgtcgccgt ccgcctcgtg tgtcggaatg
     3601 gtgcgcgaga actgctcgcc ggagctcggc tcgcgcgtct tcgagctgtc ggagttcctc
     3661 gtcacgcggc tcggcgtcga cgacgtcggc gcctactacc cgcaccgcgt cacgtaccac
     3721 ccgacctgcc attcgctgcg cgtgcttcgc gtcggcgacg cgccgctgcg cctgctgcgc
     3781 gccgtccgcg gcatcgacct cgcggagctc gagcaggcgg cggagtgctg cggcttcggc
     3841 ggcacgttcg ccctcaagaa cgctgatgtg tcgtcagcca tgctcgccga caagatcggc
     3901 cacgtgctcg agacgcgggc cgaggtgtgc gccgccgcgg acacgtcgtg cctgatgcac
     3961 atcggcggcg gtctcgcccg ccagcgggct ggtgtgcgca ccgtccatct ggccgagatc
     4021 ctggcctcca ccgaggagga gccgctgcgg tgacgagctt tccggatgcg gcacgccagg
     4081 cgctgggcga ctcacagctt cgccacaacc tgggcaacgc cacacgctcg atccgcgaga
     4141 agcgcgcccg cgccgtcggc gagctgccgg actgggagga gctgcgcaca gcgggcgccg
     4201 cgatcaagga gcacacgctc aggcgactcg attcgtacct ggtcgcgctg gagaacgcgg
     4261 tgcaggcgcg gggcggcgtg gtgcactggg cgcgcgatgg ggcagaggcg tgttccatcg
     4321 tcgctgcgct tgcccgtgac gccggcgcgt ctgaggtcgt caaggtgaag tcgctcacca
     4381 ccgacgagat cgagttgaac aaggcgctcg aggcggccgg gatcgacgcc gtcgagaccg
     4441 atctcgccga gctgatcgtg cagctcgacg gagactggcc gtcgcacatc ctcgtgccgg
     4501 ccatccaccg caaccgcgcg gagatacgca acatcttccg gacccacctg gacggcgtgg
     4561 aggcactgtc ggacgagccc tcggcgctga ccgaggcagc gcggcttcac ctacgccgga
     4621 agttcctcgc ggcgggcgtg gcggtgagcg gggcgaactt cgcggtggcc gagaccggga
     4681 ccgtgtgcgt ggtcgagtcg gagggcaacg gccgcatgtg cctcacgctg cccgaagtcc
     4741 tgatcacggt gatgggcatc gagaaggtcg tgccgagctg gcgcgacatg gaggtgttcc
     4801 tgcagctgct gccgcgttcg tcgaccgccg agcgtatgaa cccgtacacg tcgtgctgga
     4861 gcggcgtgtc cgcgggcgac ggaccgcagg agttccatct cgtgcttctc gacaacggcc
     4921 gcaccgaggt gctgcgcgat tcggtcgggc gccagacgct gcgctgcatt cgctgctccg
     4981 cctgcctcaa cgtgtgtcct gtgtacgagc gcgtgggcgg ccacgcctac ggctccatct
     5041 atcccgggcc gatcggcgcg atactcacgc cgcagctgcg cggagtcga
//
LOCUS       DASVXS010000251        16061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1026667, whole genome shotgun sequence.
ACCESSION   DASVXS010000251 DASVXS010000000
VERSION     DASVXS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026667"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..594)
                     /locus_tag="VF752_14290"
     CDS             complement(46..594)
                     /locus_tag="VF752_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8856763.1"
                     /translation="MGRIAVHEFMSLDGVYEDPSWTFEYGFDPKMGETLGAITGTSSA
                     ILLGRRTYEMFYPAWSTRTAEDDPGAPFFNDTEKYVVSGSLEEAEWNNSTILGPYDKR
                     AIQDLKDQTDGGIYVSGSGTLVRAMLDDGLVDELHLFVYPVALGTGEHLFAESGTATK
                     LALQETEPYANGVVRLSYGPAA"
     gene            complement(685..1197)
                     /locus_tag="VF752_14295"
     CDS             complement(685..1197)
                     /locus_tag="VF752_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856764.1"
                     /translation="MIAYKFLNTGGRGVFTRFSWPLPDGGPGEWVDARIVPCRSGIHA
                     CRLSDLPLWVGREMYEIELDGEVLDEPTKVVASRGRLLRRVTSWDGDARDAYTRMCAD
                     RAHELAASVSPPLDEWDAVVEPSVPEGPALLGFVAARIAEELSCAGGYRTERGRQTEW
                     LVERLGLTPA"
     gene            complement(1194..1445)
                     /locus_tag="VF752_14300"
     CDS             complement(1194..1445)
                     /locus_tag="VF752_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX8856765.1"
                     /translation="MAESADHVSCQEVVELVTDYLEGALPGDRTALFEQHLNFCEGCV
                     WYVDELRTTVSRLGEIREEHVPPDTRERLLRAFRDWNRS"
     gene            complement(1438..2097)
                     /locus_tag="VF752_14305"
     CDS             complement(1438..2097)
                     /locus_tag="VF752_14305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8856766.1"
                     /translation="MTPADAEQRLTGTASSEAKLVARLRAGDEQAFAELIDSYHGTML
                     AVARNYVNTRAVAEEVVQEAWLGVIKGLDRFQGRSSLKTWILRILVNTAMTRGGQEAR
                     SIPFSSLAREDEAEPAVEPDRFRRPDGAFPGHWAAYPANWGALPEDRVFGRETLGVMQ
                     EAIEALPTPQRVVITLRDVQGCSPEEVCDALDVSDGNQRVLLHRARSRVRAALERHFD
                     G"
     gene            complement(2136..2594)
                     /locus_tag="VF752_14310"
     CDS             complement(2136..2594)
                     /locus_tag="VF752_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856767.1"
                     /translation="MRRAANTLAIAIGKGLVAGFAGTAAMTVSSTLEARMRGRAPSSA
                     PARATAKMLGIKEFEDDIAKARFNDLSHWGYGTGWGVVRGLLGATGITPRAATAAHGA
                     AIYGAAQVTLPALEIAPPSVFWPKEEVAIDAFHHAVYAAATGVAYELIAH"
     gene            complement(2591..4186)
                     /locus_tag="VF752_14315"
     CDS             complement(2591..4186)
                     /locus_tag="VF752_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8856768.1"
                     /translation="MASDHYDVIIIGTGAGGGTLAHRLAPSGKRILVLERGGYLPREP
                     ENWDSKEVFLKERYLSGEQWYDRNGEPFRPHQQYFVGGNTKFYGAILFRLRERDFEEV
                     QHYGGVSPAWPISYGDLEPYYAQAEQLYLVHGLAGEDPTEPWRSADFPHPPVSHEPRI
                     QQLHDDFEKTGHRPFHLPVGVDLNESDPEAGRCVRCDRFDGFPCLTDGKADAHVRCVR
                     PALKHDNVTLVTHAKVERLETDASGHTISRVVVSRKGNEQVFSGDIVVVSCGAANSAA
                     LLLKSASDKHPNGLANSSDVVGRNYMAHINSGVIAISQTPNTTKFQKTLGVNDYYWGD
                     EDSDLPLGHIQMLGKSDRNILRGGAPWFAPGRALDYMSKHAIDFWLTTEDLPHPDNRV
                     TVDRQGNVHLAKMYLNNEPHRRLLAKLKGLMGPLGCHEMSIPSWSVLDQRIPLAGVAH
                     QCGTVRFGADRATSALDVDCKAHDVDNLYVVDTSFFPSSSAVNPALTAMANALRVGDH
                     LLARLDARAERFEPAPEPMEVVS"
     gene            complement(4199..5059)
                     /locus_tag="VF752_14320"
     CDS             complement(4199..5059)
                     /locus_tag="VF752_14320"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX8856769.1"
                     /translation="MTAAPSVDARWWPSPFGADDQVGMLNHITDAKRLEALASVSEGR
                     LYDLGRVLHEDIPVFPGRFFRQTLVTTAHHVNQDGGVGENNVNWVTEQVSATMQLGTH
                     IDALSHLQIGMRGYNGWSVSELAGTAGVRRLGVETVPQIVTRGWLVDAPGEPGDVIGL
                     DAVRDIEPEPGDAVLFHTGWGAHWDDPDTYVSGEPGPGMELAGWLAERGVAVTGCDTW
                     SYGPVPAEDPNRPFEVPQTLNAKHGVFVVENLDTAQVAADGVRRFALILTHPKLRGAT
                     GAWTSPIALV"
     gene            complement(5056..5904)
                     /locus_tag="VF752_14325"
     CDS             complement(5056..5904)
                     /locus_tag="VF752_14325"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX8856770.1"
                     /translation="MSEATTTVERTRGLRGLQGKNVLVTGGSSGIGQAIAVRFAEYGA
                     NVAINYLTTPDEAADTEEQVRACVAKVQREGVQDVLVQADVSNEDDAVRMVREAIEGL
                     GGIDVLVNNAGIQISRPTEELSSADFDKVLAVNLRGSFLAAREAIKHLLAEEKPGSII
                     NVSSVHQLIPKPSYLGYSTSKGGMQNLTRTLALEYAGRGIRVNGVGPGATVTPINRAW
                     IDDPEKRRQVEEHIPMRRAGDADEMAGVTAFLASDDAAYITGQTIFVDGGLTLYPSFL
                     TPWSSE"
     gene            complement(5907..7130)
                     /locus_tag="VF752_14330"
     CDS             complement(5907..7130)
                     /locus_tag="VF752_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8856771.1"
                     /translation="MPHFDDLLIVAAVAFAAPFVLGLFPRLALPSVVLEILAGIAIGP
                     SVLGIVHVDEAIEVIALIGLAFVLFLAGLEIEFPKLRGQVLRLTATGFALSFGIALVV
                     ALGLKAGGLIETPMLVAIILCATSLGVLIPVLKDAGEISTTFGQLIIAAASIADFGAI
                     ILLTIFFSGEGGIGSTLVLLGGLALLAIAVYVAVRGAEHSMRIRSDLLRLQDTTAQIR
                     VRAAIVLFVGFSAVAEKLGLEVILGAFIAGSIISLLDRDEVMTHPDFRRKLEAMGFGF
                     FIPVFFVTSGVRYDLDALTAQTSNLVMLPIFLAALLVVRGVPALLYRRVIDTRHTAIA
                     GLMQATSLPFIVAATAIGQDLGLVDAAESAALIGAGLLSVLIFPLLGLTLLRRSPVTQ
                     EQQRPLRPIAPIEET"
     gene            complement(7185..7457)
                     /locus_tag="VF752_14335"
     CDS             complement(7185..7457)
                     /locus_tag="VF752_14335"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX8856772.1"
                     /translation="MIEVEGGRRVVTLTRFETSAGPDLRVYLAPAGADQDSRGEGSVD
                     LGGLKGNIGNQQYDIPRDADLGRLTTVIVWCRAFSVGFGAAYLRAA"
     gene            complement(7390..7908)
                     /locus_tag="VF752_14340"
     CDS             complement(7390..7908)
                     /locus_tag="VF752_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856773.1"
                     /translation="MQSPSVETPRRRRTPTAVRWVSVPVVVVVLLAGIWVAGGLITND
                     FKVSMLLTVAWGALAGLACFVLVLKRREMWPALAAYLVTAAAAGIYLGTQTLIDRKVD
                     EKVVTGRAGDRAAGRARHSQRTRRARRQPAPLARRVRVARTRHEWDRSGDRGRRWPAR
                     GHADPLRDERRP"
     gene            8099..8467
                     /locus_tag="VF752_14345"
     CDS             8099..8467
                     /locus_tag="VF752_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8856774.1"
                     /translation="MPELELLVARVESAIASSEVVALVAGHPPLGVALLTFRPSIWTG
                     APTATLEDLYVVPQQRGRGHGRSLLRGALDAARQRGAASVELYTGEGDAAARALYEAH
                     GFMTTEPGESDRLFFYHRRL"
     gene            8796..10676
                     /locus_tag="VF752_14350"
     CDS             8796..10676
                     /locus_tag="VF752_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8856775.1"
                     /translation="MTETSLLPHAPPGAARATDMFQPIAEYGLLADCNTAALVDRSGS
                     IDWLCLPRYDSDAVFSRLLGPEAGHWYIRPAGAFEVERRYLPGTLVIETTFKTSTGAV
                     RLYDALAFADGQRSHDLGFDVPHELLRSVEGVSGSIELELELAPRPEYGLVKPLFRLE
                     DGGGRTFGGSGRVAVRAGVPVEIEDSTMRASFILREGETVGFSLRWAPPEERSLEPLP
                     AEQVPARILDVAEGWRSWEAEHDIYEGPHRELVRFSSRVLKGLTYRPTGAVVAAPTTS
                     LPETVGGERNWDYRFAWIRDASLTLEALYVGACSDEASEFVSFMTSAAGGRAREGSLQ
                     IMYGIDGRHDLSERQLAHLPGWRNSAPVRVGNGAWNQVQLDVYGELLNSLYLYRDKLG
                     DLHMEIQAFIADLADTAARRWREKDSGIWEMRGEPRHHLSSKVMCWVALDRAVKLAPS
                     LGNYAKEDEWSAARDEIRAAIEERGWSEAAQAYAQSFDSDELDGAALLMPIMGFLPAT
                     DERMRSTIEAIARELTQDGMVLRYRNEEGLNADGLSGEEGTFVICTWWLVSCLAQAGE
                     VERAEELFEQLAGFANDLGLIGEEVDAQSGEQLGNFPQAYSHIGLINAAYEIDKARGA
                     VA"
     gene            10673..12031
                     /locus_tag="VF752_14355"
     CDS             10673..12031
                     /locus_tag="VF752_14355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8856776.1"
                     /translation="MSGAREYDVIVLGGGPAGEHCAARLADGGASVAIVERELLGGEC
                     SYWGCIPSKTLLRPGEALQGAREAPGAREAVKGGIDAEAAFAWRDFMVSDYSDAGQAA
                     WAEGAGIDVIRGSGRLAGPGRVEVGDDVFAARDIVISTGSEPVIPPIDGLRELEGVWT
                     NREATALNEVPRRLVVLGGGPIGVEMAQTVSRMGGSVALVEGMDHLLPREPRALGDAL
                     GEILSGEGVELCFGQLASAVRRDDGEFVVEFPERSELRGDRLLVATGRRPRHRDIGLE
                     TVGIEPARNGIEVDARMNAGDGIWAIGDVTGIWPLTYVGKYQGRVAAANILGLAREAN
                     YDAVPRVVFTDPQAAAVGESEGPLMATARLSEVPRTATYTRAYAEKPGFMTLVSDGER
                     LTGARAVGPDAGEWLQQATVAIRARVPLDVMDDVIQPFPTFSEVFLHALLELRANVPA
                     PA"
     gene            12073..13023
                     /locus_tag="VF752_14360"
     CDS             12073..13023
                     /locus_tag="VF752_14360"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8856777.1"
                     /translation="MAIAHTPRTAFTVHALTRVVATDAHEGPVYVAQDDALYFTTVPR
                     AGDVPVPGESSVAIERVALDGGSFPLEPERVSAIRADANMANGMALDLEGRLVVCEQG
                     TRSPHAAITRLDRKTGTREILVDSFDGLPLNSPNDVVVKSDGTIWFTDPSYGWLQGFR
                     PRPQQPDAVYRYDPRDGSLTLVTDSLDKPNGLAFSPGETVLYVGDSGANHEPGSYEPT
                     RPHHVMAFDVVQGRELSDERVLREITPGFPDGIKVDSQGRLYVSSFSGVQVLDPSGER
                     IGEIELAGAVNFTFGGPDRDVLFITADDAIWAAHLDATGP"
     gene            13045..13881
                     /locus_tag="VF752_14365"
     CDS             13045..13881
                     /locus_tag="VF752_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX8856778.1"
                     /translation="MAAVRTRRTIDTEGADAVIAAAEEYANERGHRVVMCVVDPWGEV
                     VQIRRTSGAQIASSRVAVDKARTAAIFVRPSREIEEQVTNGRLGALALHGASALIGGI
                     PLTVDGEVVGAIATSGETPDEDEAVSLAGAAAAFSTEEFPALTQEGAKLAAEAIGAEA
                     ARRGVAPVVSAVDAGGALMYLHRPDEAQVASVEVTTDKARTAAIYRRPSKDFEDQASG
                     GRPSALHLARAVPLQGGEPIVYEGRVIGAVGVSGATDADEDRELALLGAAAIANGVEV
                     KA"
     gene            13881..15629
                     /locus_tag="VF752_14370"
     CDS             13881..15629
                     /locus_tag="VF752_14370"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8856779.1"
                     /translation="MATLTATPASRLPGPPEVLPGKPDSVIDLQTDAGAGLAGVEWRY
                     ADARVEEVDFVELAGPGAADPLGPGDVPNRTYDVLPHAEGADYDDSAWRVLAPDETRA
                     RLGNGRVSFNWYRTELTVPAQIGDHDPTGGTLVFEVVVDDYAEVWVNGELPLVLGYAG
                     GQVVGGFNAPNRVVLTRDARPGQRFQIAVFGINGPISVSPHNYIWMRTATVEFHACGR
                     APIAFERPLEVEREDSRIDRIVPPDAALEQVAGGFEFTEGPLWSPKGALLFSSPNTNV
                     MYRWDASTGTVSIFNAKSGYTGVDIGRFGQPGSNGLTFDDHGRLVMCQHGNRRVVRVN
                     PHGDTTVLAERFDGRRLNSPNDLVFRSDGTLYFTDPPFGLPEMFGDPAKELPFSGVYR
                     VRDGAVSLETDELDGPNGLAFSPDERFLYVGNWDPDRKVVMRYEVGSDGALHNGTVLH
                     DMTGAPGEDAIDGLKVDEAGNVYACGPGGIWVISPEGERLGLIQLPEAPHNLAFGDDD
                     RRTLYITALTGVYRLRLGVAGARPAWQARKETHEQGTRTRNDVPRRDAAGRERHAPPA
                     VDAPGRRHARASSQPR"
     gene            15526..>16061
                     /locus_tag="VF752_14375"
     CDS             15526..>16061
                     /locus_tag="VF752_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856780.1"
                     /translation="MTFPDVTLPDENGTRHRLSTLQGDDMLVLHLSRGEHCPRERMHH
                     RELLRFHEWCSVAFTQLVSILPNTLHDTYKMKIATGAHWTFLADEGLELREALEIDEY
                     TDNHHDNAVVPHTLILSPGLRIEKVYVGYWFWGRPSPEQLWLDLQDLTRRAKADYDPT
                     LPDVRAELQVADAARAAA"
BASE COUNT         2506 a         5471 c         5644 g         2440 t
ORIGIN      
        1 tcataccttc ggcgcgcgct gtcggagatc gagaagccgt agcccctacg cggcggggcc
       61 gtaggacagg cgcacaacgc cgttggcgta gggctcggtc tcctgcagcg ccagctttgt
      121 cgccgtgccg ctttcagcga acaagtgctc gccggttcca agggcgaccg gatagacgaa
      181 caggtggagc tcgtcgacga gaccgtcgtc cagcatcgcc cggacgagcg ttccgctgcc
      241 gctcacgtag atgccgccgt cggtctggtc cttcagatcc tggatcgcac gcttgtcgta
      301 cgggccgagg atcgtcgagt tgttccactc ggcttcctcc agactccctg agacgacgta
      361 cttctcggtg tcgttgaaga agggagcacc gggatcgtcc tcagcggtcc gagtcgacca
      421 cgcgggatag aacatctcgt aggtgcggcg cccgagcagg atcgcagagc tcgttccggt
      481 gatggccccg agcgtctcgc ccatcttggg gtcgaagccg tattcgaacg tccagctcgg
      541 gtcctcgtag acgccgtcga gactcatgaa ttcgtgtacg gcgatcctgc ccatggccat
      601 tctccttgtc gtgagctgcg aatgagtgtg cctcgcagtc tgaagaccgc gctacgcaga
      661 gaaagtcatc gcccgcgttg gtcgttacgc cggcgtgagc ccgagtcgct ccacgagcca
      721 ctcggtctgg cggccgcgct cggtgcgata cccaccggcg cacgagagct cctccgcgat
      781 cctcgccgcc acgaaaccga gcaacgccgg cccctcgggg acggacggct ccacgacagc
      841 gtcccactcg tcaagcggcg gcgagacgct cgcggccagc tcgtgcgcgc ggtccgcgca
      901 catgcgcgtg tacgcgtcgc gcgcgtcgcc gtcccaactg gtcacccggc ggagcagccg
      961 cccgcgggag gccacgacct tcgtcggctc atcgaggacc tcgccatcga gctcgatctc
     1021 gtacatctcg cggccgaccc acagcggcag gtcggacaga cggcatgcgt ggatgccgct
     1081 gcgacacggc actatgcgtg cgtcgaccca ctcgcccggg ccgccgtcgg gaagcggcca
     1141 gctgaaccga gtgaagacac cgcggccgcc cgtattcaga aacttgtagg cgatcacgag
     1201 cggttccagt cgcggaacgc cctcaggagg cgctcgcgcg tgtcgggcgg gacatgctcc
     1261 tcgcgaatct cgccgagccg cgagacagtt gtgcgcagct cgtcgacgta ccagacgcag
     1321 ccttcgcaga agttcagatg ctgttcgaac agcgcggtgc ggtcgcccgg cagcgcgccc
     1381 tcgaggtagt ccgtcacgag ctcgacgact tcctggcagg agacgtgatc ggcggattca
     1441 gccatcgaaa tgcctctcga gtgcggcgcg cacacgtgag cgtgcgcgat ggagaaggac
     1501 ccgctggttg ccgtccgaga cgtcgagcgc gtcgcacacc tcttccgggc tgcagccctg
     1561 gacgtcacgg agcgtgatca ccactcgctg cggcgtcggc agcgcctcga tcgcttcctg
     1621 catgacgccg agcgtctcac gcccgaagac ccgatcctcc ggaagggcgc cccagttcgc
     1681 cggataggcg gcccagtggc ccgggaaggc gccgtccggc cggcggaagc ggtccggctc
     1741 gaccgcaggc tcagcctcgt cctcgcgcgc gagcgacgag aaggggatgc tgcgcgcctc
     1801 ctggccaccc cgcgtcatcg cggtgttgac gaggatgcgg aggatccacg tcttgagcga
     1861 cgaccgtccc tggaagcggt cgaggccctt gatgacgcca agccacgcct cctgcacgac
     1921 ctcctcggcc acggctcgcg tgttcacgta attacgcgcc acggcgagca tggtcccgtg
     1981 gtagctgtcg atgagctcgg cgaacgcctg ctcgtcgccc gcccgaaggc gtgcgaccaa
     2041 cttcgcctcc gaggaggcgg tgccggtcag ccgttgctcg gcgtcggcgg gagtcacggg
     2101 ctcggcgtga acgtagttgc agtgggcccc ccggtctagt gcgcgatcag ctcgtatgcg
     2161 acgccggtcg cggcggcgta tacggcgtgg tggaaggcgt cgatggccac ctcctccttc
     2221 ggccagaaaa ccgatggggg cgcgatctcg agcgcgggca gcgtcacctg cgccgcgccg
     2281 tagatcgcgg cgccgtgtgc ggccgtcgcg gccctcggcg tgatgcccgt cgcccctagc
     2341 agcccgcgga ccacgcccca cccggtgccg tagccccagt gggagaggtc gttgaagcgc
     2401 gccttggcga tgtcgtcctc gaactccttg atgccgagca tcttcgcggt ggcgcgggca
     2461 ggcgccgacg acggcgcgcg cccacgcatc ctcgcctcga gtgtgctcga gacggtcatc
     2521 gcggctgtgc ccgcgaaccc ggcgacgagg cccttgccga tcgcgatagc gagcgtgtta
     2581 gctgcccttc tcatgagacc acctccatcg gctccggtgc gggttcgaag cgctcggcac
     2641 gtgcgtcgag tcgcgccagc aggtggtcac ccacgcggag tgcgtttgcc atcgccgtga
     2701 gggcgggatt caccgcactc gacgagggga agaagctcgt gtcgacgacg tagaggttgt
     2761 cgacatcgtg cgccttgcag tcgacgtcga gcgccgacgt cgcgcggtcc gcgccaaagc
     2821 gcacggtccc gcactgatgg gcgacaccgg ccagcgggat ccgctggtcg agcacggacc
     2881 aactcgggat cgacatctcg tggcagccga gggggcccat caggcccttc agcttcgcga
     2941 gcagtcgccg gtgaggctcg ttgttgagat acatcttcgc caggtggacg ttgccctggc
     3001 ggtcgacggt cacacggtta tccgggtgcg gcaggtcctc cgtcgtgagc cagaagtcga
     3061 tcgcgtgctt cgacatgtag tcgagcgcac gaccgggcgc gaaccacggc gcgccgccgc
     3121 gcaggatgtt gcggtcggac ttgccgagca tctggatgtg cccgagcggc aggtccgagt
     3181 cctcgtcgcc ccagtagtag tcgttgacgc cgagggtctt ctggaacttg gtcgtgttcg
     3241 gggtctgcga gatcgcgatc acgcccgagt tgatgtgcgc catgtagttc cgaccgacga
     3301 cgtctgacga attcgcgagc ccgttggggt gcttgtcaga ggccgacttg agcaggagag
     3361 ccgccgagtt cgcggccccg caggaaacga cgacgatgtc gccgctgaag acctgctcgt
     3421 tccccttgcg gctcacgacc acgcggctga tggtgtggcc ggaggcatcc gtttcgaggc
     3481 gctcgacctt ggcgtgggtg accagcgtga cgttgtcgtg cttcagcgcc ggccgcacgc
     3541 accgcacgtg ggcgtcggcc ttgccgtcgg tgaggcaggg gaagccgtcg aagcggtcgc
     3601 agcgcacgca gcggccggcc tcgggatcgg actcgttcag gtcgacgccg acaggcaggt
     3661 ggaacgggcg atggcccgtc ttctcgaagt cgtcgtgcaa ctgctggatg cgcggctcgt
     3721 gcgacacggg cgggtgggga aaatcggcgg agcgccacgg ctccgtcgga tcctctccgg
     3781 cgagcccgtg cacgagatag agctgctccg cctgggcgta gtacggctcg aggtcgccgt
     3841 aggagatggg ccaggcgggg gagacgccgc cgtagtgctg cacctcctcg aaatcgcgct
     3901 cgcgtagccg gaacaggatc gcgccgtaga acttcgtgtt gccgccgacg aagtactgct
     3961 ggtgcggccg gaacggttcg ccgttcctgt cgtaccactg ctcgccggag aggtagcgct
     4021 ccttgaggaa gacctctttc gagtcccagt tctccggctc gcgcgggagg tagccgccgc
     4081 gctccagcac caggatgcgc ttgccggacg gcgcgagcct gtgcgcaagc gtgccgccac
     4141 cggccccagt gccgatgatg atcacgtcgt agtggtcgct ggccatgcga gtcctccttc
     4201 agacgagtgc gatcggggag gtccaggcgc ccgtggcgcc gcggagcttc gggtgggtca
     4261 ggatcagcgc gaagcggcgc acgccgtcgg cggcgacctg cgcggtgtcg aggttctcga
     4321 cgacgaagac gccgtgcttg gcgttcaggg tctgcggcac ctcgaagggg cggttggggt
     4381 cctcggcggg caccgggccg tagctccatg tgtcgcagcc agtcaccgcc acgccgcgtt
     4441 cggcgagcca gccggcaagc tccattccgg gccccggctc gccgctcacg taggtatccg
     4501 ggtcgtccca gtgcgcgccc cagccggtgt ggaaaagcac cgcgtcgccg ggctctggtt
     4561 cgatgtcacg cacggcatcg aggccgatca cgtcgccggg ctctcccggc gcgtcgacca
     4621 accagccgcg cgtcacgatc tgcggcaccg tctccacgcc gagccttctc acgcctgcgg
     4681 tgccggccag ctccgatacc gaccagccgt tgtagccacg catgccgatc tgcaggtggc
     4741 tgagcgcatc gatgtgggtg ccgagctgca tcgtggccga cacctgctcc gtcacccagt
     4801 tcacgttgtt ctcgcccacg ccgccgtcct ggttgacgtg gtgcgcggtg gtcacgagcg
     4861 tctggcggaa aaagcgtccg gggaagaccg ggatgtcctc gtggaggacg cggcccaggt
     4921 cgtacagccg tccctcgctc accgacgcga gcgcctcgag gcgcttagcg tcggtgatgt
     4981 ggttgagcat cccgacctga tcgtcggcgc cgaacggcga cggccaccag cgggcgtcaa
     5041 ctgagggggc cgcggtcatt cggacgacca gggggtgagg aagctcgggt agagcgtcag
     5101 gccgccgtcg acgaagatcg tctggccggt gatgtaggcg gcgtcgtcgc tggcgagaaa
     5161 tgcggtcacg cccgccatct cgtcggcgtc gccggcgcgc cgcatcggaa tgtgctcctc
     5221 cacttgccgg cgcttctccg ggtcgtcgat ccaggcacga ttgatcggcg tcaccgttgc
     5281 gcctggaccg acgccgttca cgcggatgcc gcgaccggcg tactcgagcg cgagagtgcg
     5341 ggtgaggttc tgcatcccgc ccttgctcgt cgagtagccg aggtaggacg gcttcgggat
     5401 cagctggtgg acgctcgaga cgttgatgat cgagcccggc ttctcctcgg cgaggaggtg
     5461 cttgatcgcc tcgcgcgcgg ccaggaacga gccgcgcagg ttcaccgcga gcaccttgtc
     5521 gaagtccgcg ctcgacagct cctcggtggg gcgagagatc tggatgcccg cgttgttcac
     5581 gagtacgtcg atgccgccga ggccttcgat cgcctcgcgc accatgcgca ccgcgtcgtc
     5641 ttcgttcgac acgtcggcct ggacgaggac gtcctgcaca ccctcgcgct gcaccttggc
     5701 gacgcacgcg cgcacctgct cctcggtgtc ggcggcctcg tccggtgtgg tcaggtagtt
     5761 gatggcgacg ttcgctccgt actccgcgaa gcgaacggcg atcgcctggc cgatgcccga
     5821 gctgccgccg gtgacgagca cgttcttgcc ctggaggccg cgcaggcctc tggtccgctc
     5881 gacggttgtc gttgcttcgc tcatcttcac gtctcctcga tcggggcgat ggggcgtagt
     5941 ggccgctgct gctcttgcgt tacaggcgat cggcgcagca acgtcaggcc gagcagcggg
     6001 aagatcagga cggacagcag gccggcgccg atcagggcgg cgctctcggc ggcatcgacc
     6061 aggcccaggt cctgcccgat cgccgtggcg gcgacgatga acgggagcga ggtggcctgc
     6121 atgaggcccg cgatcgcggt gtggcgtgtg tcgatcacgc gccggtagag cagcgccggc
     6181 accccgcgca ccaccagcag cgccgccagg aagatcggca gcatcacgag gttcgaggtc
     6241 tgcgcggtca gcgcgtcgag gtcgtagcgg acgccgctcg tgacgaagaa gaccgggatg
     6301 aagaagccga accccatcgc ctccagcttc cggcggaagt cggggtgcgt catcacctcg
     6361 tcgcggtcga gcagcgagat gatcgagccc gcgatgaacg cgccgaggat gacctcgagg
     6421 ccgagcttct ccgccacggc cgagaagccg acgaagagga cgatcgcggc gcggacgcgg
     6481 atctgcgccg tggtgtcctg gagccggagc agatcggagc ggatccgcat cgagtgctcc
     6541 gccccgcgaa ccgccacgta gaccgcgatt gcgagcagcg cgagcccgcc gagcagcacg
     6601 agcgtggatc cgatcccgcc ctcgcccgag aagaagatcg tcagcaggat gatcgccccg
     6661 aagtcggcga tcgaggccgc ggcgatgatg agttggccga aggtcgtgga gatctctccg
     6721 gcgtccttca gcaccgggat caggacgccg agcgaagtcg cgcacaggat gatcgcgacg
     6781 agcatcggcg tctcgatcag gccgcccgcc ttgagcccga gcgcgaccac gagtgcgatc
     6841 ccgaacgaga gcgcgaaccc cgttgcggtc agtcgcagca cctgaccgcg cagcttcggg
     6901 aactcgatct cgaggccggc gaggaacagc acaaacgcca ggccgatgag ggcgatcacc
     6961 tcgatcgcct cgtcgacgtg gacgatgccg agcaccgatg gaccgatggc gatcccagcg
     7021 aggatctcga ggaccaccga gggcagcgcc agacgtggga acagtccgag gacgaagggc
     7081 gcggcgaacg cgaccgccgc cacaatcagt aggtcatcga agtgcggcat gccctgtggt
     7141 ccaacgaagt gcgaccgcgt tacgcgactt cgagctcggg ttcatcaggc cgctcgcagg
     7201 tatgcagcgc cgaagccgac tgagaaggcg cggcaccaca cgatcaccgt tgtgagccgg
     7261 ccgaggtcgg catcgcgcgg gatgtcgtac tgctgattgc cgatattgcc cttgaggccg
     7321 ccgaggtcga cggagccctc gccgcgcgag tcctggtcgg cgcctgcagg cgcgaggtag
     7381 acgcgcaggt cagggccggc gctcgtctcg aagcgggtca gcgtgaccac gcgccggcca
     7441 ccttcgacct cgatcacctg agcggtccca ctcgtgccgt gttcgagcga ctcgaactcg
     7501 ccgcgcgaga ggagccggtt gccggcgggc gcgtcgcgtc cgctgcgagt gtcgcgctcg
     7561 ccctgctgcg cggtcgccgg cgcggcccgt gaccaccttc tcatcgacct tgcggtcgat
     7621 cagcgtctgc gtgccgaggt agatgccggc tgccgccgca gtgaccaggt acgccgcgag
     7681 cgccggccac atctcgcggc gcttcaagac gaggacaaag cacgcgagcc cggcgagcgc
     7741 gccccacgcg acggtgagca gcatcgagac cttgaagtcg ttcgtgatca gcccgccggc
     7801 gacccagatc cccgccagga gaacgaccac gacgacgggc acggagaccc agcggaccgc
     7861 ggtcggcgta cgccgtcggc ggggcgtctc gacgctcggc gattgcatgc aaccgaagtc
     7921 ccacccgcga ggcgtgtgtt acgcgccggt ttgatcggcg ggtcgtaacg agatccacgg
     7981 caggattgac ctgaggaccg atggggtccg atgccgcaag cgctcgaatt gcgtcagcga
     8041 aggagagtcg tgtcgtgggg cgctcctcca ccagttcgcg gccgagttcg gcgatccgat
     8101 gccggagctg gagctactcg tcgcgcgcgt cgagtcggcg atcgcctcct ccgaggtggt
     8161 cgcactcgtc gctggtcacc cgccgctcgg cgtcgccttg ctcaccttcc gtccctcgat
     8221 ctggaccgga gcgccgaccg ccacgctgga ggacctctac gtcgttccgc agcagcgcgg
     8281 gcgcggacac ggtcgttccc tgcttcgcgg agccctcgat gccgctcgtc agcgcggcgc
     8341 cgcgtcggtc gagctttaca ccggcgaggg tgatgccgcg gcccgcgcgc tctatgaggc
     8401 gcacggcttc atgaccaccg aacccggcga aagcgacagg ctgttcttct atcaccggcg
     8461 cttgtagccg tcacaccgct atgcgccgcg agcgaagctc ccgcaacgcc cggagcgcga
     8521 accgatcgag cgcccgcggt cattcgctga ggcggaccga cctgggccgt tttgacattg
     8581 atagtcatgg tcaaaacagc ccaccctctt cacttcggcg ggccgagaac ggacctgggc
     8641 tggcaaaccg gccgcgcaac caccatccgc ggcccatatg tgcgggatgt cgtagcacct
     8701 tccgtgcatc gagcccggct ccggtcgcgc actcgcgcct gtaacgaagc gcgcccgccc
     8761 cggaccaagc gagcggactc gatccggagg aaggcatgac cgagacttcg ttgctcccgc
     8821 acgcaccccc gggcgccgca cgcgccactg acatgttcca gcccattgcc gagtacggcc
     8881 tgctggcgga ctgcaatacc gccgcgctcg tggaccgctc tggctccatc gactggctct
     8941 gcctgccccg ctacgacagc gacgcggtgt tctcacggct gctgggaccg gaggccggcc
     9001 actggtacat acgccccgcc ggcgcgttcg aggtcgaacg gcgatacctg ccgggcacgc
     9061 tcgtcatcga gacaaccttc aagacctcga ccggcgcagt gcggctgtat gacgcgctgg
     9121 cgttcgcgga cggccagcgc tcccacgacc tcggtttcga cgtgccgcac gagttgctgc
     9181 gttcggtcga aggcgtgtct ggcagcattg aactcgagct cgagctggcg ccgcgtcctg
     9241 agtatggcct cgtgaagccg ctgttccggc tcgaggacgg cggcgggcgg accttcggcg
     9301 gatcgggccg cgtcgctgtt cgggcgggcg tgcccgtcga gatcgaggac tcgacgatgc
     9361 gcgcttcgtt catcctccgt gaaggcgaga ccgtcggctt ctcgcttcgc tgggccccgc
     9421 ccgaggaacg ctcgctcgag ccgctcccgg ccgagcaggt accggcgcga atcctcgacg
     9481 tggcggaggg ctggcgctcc tgggaagccg agcacgacat ctacgagggc ccgcaccgcg
     9541 agctcgttcg cttcagcagc cgtgtgctca aggggctgac ctaccgcccg accggcgcgg
     9601 tggtcgccgc gccgaccacg tcgctgccgg agacggtcgg cggcgagcgc aactgggact
     9661 accgcttcgc ctggatccgc gacgcgagcc tcacgctcga ggcgctctac gtcggcgctt
     9721 gcagcgacga ggcgtcggag ttcgtctcgt tcatgacgag cgccgcgggc ggccgtgcgc
     9781 gagaagggtc gcttcagatc atgtacggca tcgacgggcg gcacgacctg tcggagcgcc
     9841 agctggcgca cctaccggga tggcgcaact cggcgcccgt gcgcgtggga aacggcgcgt
     9901 ggaaccaggt gcagctcgat gtctacggcg agttgctcaa ctcgctgtac ctgtaccgcg
     9961 acaagctcgg cgacctccac atggagatcc aggcgttcat cgccgacctc gccgacaccg
    10021 cagcgcggcg ctggcgcgag aaggactccg gcatctggga gatgcgcggt gagccgcggc
    10081 accacctgtc gtcgaaggtg atgtgctggg tcgcgctcga ccgagccgtg aagctggcgc
    10141 cttcgctcgg aaactacgcc aaggaggacg agtggtcggc tgcgcgcgac gagattcgcg
    10201 ccgccatcga ggagcgcggc tggagcgagg cagcgcaggc gtacgcccag tcgttcgact
    10261 cggacgagct cgacggcgcg gcgctcctga tgccgatcat gggcttcctg cccgcgaccg
    10321 acgagcggat gcgctcgacg atcgaggcga tcgcccgcga gctcacgcag gacggcatgg
    10381 tgctgcgcta ccgcaacgag gagggcctca acgccgacgg cctcagcggg gaggagggga
    10441 cgttcgtgat ctgcacgtgg tggctcgtct catgcctggc gcaagcgggc gaggtcgagc
    10501 gcgccgagga gctgttcgag cagctcgccg gctttgcgaa cgacctcggc ctgatcggcg
    10561 aggaagtgga cgcgcaaagt ggcgagcagc tcggcaactt cccgcaggcc tacagccaca
    10621 tcgggctgat caacgccgcc tacgaaatcg acaaggcgag aggagccgtg gcatgagtgg
    10681 cgcgcgcgaa tacgacgtga tcgtcctcgg cggagggccc gcgggtgagc attgcgccgc
    10741 ccggctcgcc gacggcgggg ccagcgtggc gatcgtcgag cgcgagctgc tcggcggcga
    10801 gtgctcgtac tggggctgca tcccgtcgaa gacgctgttg cgtccgggcg aggcgctcca
    10861 gggcgcgcgc gaggcgcccg gcgcgcgcga ggccgtgaag ggcggcatcg acgccgaggc
    10921 cgccttcgcc tggcgcgact tcatggtcag cgactactcg gacgcgggcc aggccgcctg
    10981 ggccgagggc gcgggcatcg acgtgattcg cggctccggc cggcttgccg ggcccgggcg
    11041 cgtggaggtc ggagacgacg tgtttgccgc gcgggacatc gtcatctcga cgggttccga
    11101 accggtcatc ccgccgatcg acgggctccg cgagctcgaa ggcgtgtgga cgaaccgcga
    11161 ggccaccgcc ctgaacgagg taccgcgacg gctggtggtg ctcggcggcg ggccgatcgg
    11221 tgtggagatg gctcagaccg tctcacggat gggcggctcg gtcgcgctcg tcgagggcat
    11281 ggatcacctg ctgccacgcg agccccgcgc gctcggcgac gcgctcggcg agattctcag
    11341 cggcgagggc gtcgagctct gcttcgggca gctcgcctcc gccgtccgcc gcgacgacgg
    11401 cgagttcgtg gtcgagttcc ctgagcgcag cgagctccgc ggcgatcgcc tgctcgtcgc
    11461 cacgggacgg cgtccgcgcc atcgcgacat cggcctcgag actgtcggga tcgagccggc
    11521 ccgcaacggc atcgaagtcg acgcgcgcat gaacgcgggc gacggcatat gggcgatcgg
    11581 cgacgtgacg ggcatctggc cgctcaccta cgtgggcaag taccaaggcc gcgttgccgc
    11641 ggcgaacatc ctcggcctcg cgcgcgaggc gaactacgac gcggtgcccc gcgtcgtgtt
    11701 caccgatccc caagctgccg cggtgggcga gtccgaggga ccgctcatgg cgacggcgcg
    11761 gctctccgag gtgccccgca ccgcgactta tacgcgcgcg tatgccgaga agcccggctt
    11821 catgacgctc gtctcggacg gcgagcgcct gaccggcgca cgtgccgtcg gccccgacgc
    11881 gggcgaatgg ctccagcagg ccacggtcgc gatccgggcg cgggtgccgc tcgacgtgat
    11941 ggacgatgtc atccagccct tcccgacctt ctccgaggtc tttctccacg cgctgctcga
    12001 gctgcgggcg aacgttcccg cgcccgcata gccggcccgt aacagcggcg ccgctgccag
    12061 gacaacatca ccgtggccat cgctcacacc ccgaggaccg ccttcaccgt gcacgcgctc
    12121 acgcgggtcg tcgcgaccga cgcccacgag ggcccggtgt acgtcgcgca ggacgacgcg
    12181 ctgtacttca ccaccgttcc tcgcgccggg gacgtgcccg tcccgggaga gagctcggtg
    12241 gcgatcgagc gtgtcgcgct cgacggcggt tcgttccctc tcgagcctga gcgcgtctca
    12301 gcgattcgcg cggacgcgaa catggcgaat ggcatggccc tcgacctcga gggacgcctg
    12361 gtggtctgcg agcagggcac gcgatcgccg catgccgcca tcacgcgact cgaccggaag
    12421 accgggacgc gggaaatcct cgtcgactcg ttcgacgggc tcccgctcaa ctcgccaaac
    12481 gacgtggtgg tcaagagcga cggcacgatc tggttcacag accccagcta cggctggctg
    12541 cagggattcc ggccgcgccc acagcagccc gacgccgtct atcgctacga cccgcgcgat
    12601 ggatcgctga cgctcgtcac agattcgctc gacaagccga acgggctcgc gttctcgcct
    12661 ggggagacgg tcctctatgt cggcgacagc ggcgccaacc acgagccggg cagctacgag
    12721 cccactcgcc cgcatcacgt gatggcgttc gacgtggtcc aggggcgcga gctcagtgac
    12781 gagcgcgtgc tgcgcgagat cacgccgggg ttccccgacg gcatcaaggt cgactcacag
    12841 ggacgcctgt acgtgtcgtc gttcagcggc gtgcaggtgc tcgacccgag cggcgagcgc
    12901 atcggcgaga tcgagctggc gggtgccgtg aacttcacct ttggcggccc ggatcgcgac
    12961 gtcctcttca tcactgcgga cgacgcgatc tgggccgcgc acctcgacgc aaccggcccg
    13021 tagaccacga agggagcctg acccatggcc gccgtgagga ctcgcaggac gatcgacacc
    13081 gagggagcgg acgcggtgat cgcggccgcg gaggagtatg cgaacgagcg cgggcaccgc
    13141 gtggtgatgt gcgtggtgga cccgtggggc gaggtcgtcc agatccgccg cacaagcggc
    13201 gctcagatcg cgagctcgcg cgtggccgtc gacaaggcac gcacggcggc gatcttcgtg
    13261 cgcccgagcc gcgagatcga ggagcaggtg acgaacggcc ggctcggtgc tctcgcgctg
    13321 cacggcgcca gcgcgctcat cggcgggata ccgctcacgg tcgacggcga ggtcgtcggc
    13381 gcgatcgcca cgagcggcga gacaccggac gaggacgagg ccgtgtcgct cgcgggcgcg
    13441 gcggccgcat tctccaccga ggagtttccg gcgctgacgc aagagggtgc gaagctcgcg
    13501 gcagaggcaa ttggtgccga ggcggctcgg cgcggcgtgg cgccggtcgt gtcggccgtt
    13561 gacgccggcg gagcgctcat gtacctgcat cgacccgacg aggcgcaggt ggcgagcgtc
    13621 gaggtgacga ccgacaaagc gcgcaccgcc gcgatctacc gccgcccgag caaggacttc
    13681 gaggaccagg cgtccggcgg gcggccgtcg gcgctgcacc tggcccgcgc ggtcccgctg
    13741 cagggcggcg agccgatcgt ctacgaggga cgcgtgatcg gcgcagtggg cgtcagcggc
    13801 gccacggacg ccgacgagga tcgcgagctt gcgctgctcg gcgcggcggc gatcgcgaat
    13861 ggcgtggagg tgaaggcgtg atggcgacgc tcacggccac ccccgcgtcg agactgcccg
    13921 gcccgcccga ggtcctgccc gggaagccgg actcggtgat cgacctccaa acggacgccg
    13981 gcgctgggct cgccggcgtc gagtggcgct acgcggacgc gcgcgtcgag gaggtcgact
    14041 tcgtcgagct cgccggacca ggtgcggctg acccgctcgg tcccggcgac gtcccaaacc
    14101 gcacgtatga cgtactgccg catgccgagg gcgcggacta cgacgactcc gcgtggcgcg
    14161 tgctggcccc cgacgagacg agggcgcggc ttggcaacgg ccgcgtctcg ttcaactggt
    14221 atcgcactga gctgaccgtg cctgcgcaga tcggcgacca cgatccgact gggggcacgc
    14281 tcgtgttcga ggtggtcgtc gacgactacg cggaggtctg ggtgaacggc gagctaccgc
    14341 ttgtgctggg ctacgccggc ggccaggttg tcggcggctt caacgcgccg aaccgcgtgg
    14401 tgctcacacg cgatgcgcgt ccggggcagc gattccagat cgccgtgttc ggcatcaacg
    14461 ggccgatctc cgtctcgccc cacaactaca tctggatgcg gaccgcgacc gtcgagttcc
    14521 acgcgtgcgg gcgcgcgccg atcgcgttcg agcgcccgct cgaggtggag cgcgaagaca
    14581 gccgcatcga tcggatcgtc ccgccggacg ccgctctgga acaggtggcc ggcggcttcg
    14641 agttcaccga ggggccgctg tggtctccga aaggcgcgct gctgttcagc tcgccgaaca
    14701 cgaacgtgat gtaccgctgg gacgcgagca cgggcacggt cagcatcttc aacgcgaaga
    14761 gcggctacac gggcgtcgac atcgggcgct tcggacaacc cggctcgaac gggctgacgt
    14821 tcgacgacca cggccggctc gtcatgtgcc agcacggaaa ccggcgtgtt gtacgcgtca
    14881 acccgcacgg cgacacgacg gtgctcgcgg agcgcttcga cggaaggcgg ctgaacagcc
    14941 cgaacgacct cgtgttccgc tccgacggca cgctgtactt cacagatccg ccgttcggac
    15001 tgcccgagat gttcggcgat cccgccaagg agctgccgtt cagcggcgtc taccgcgtcc
    15061 gcgacggcgc ggtgagcctg gagacggacg agctcgatgg tccgaacggg ctcgcgttct
    15121 cgcccgacga gcgcttcctc tacgtgggga actgggatcc cgaccgcaag gtcgtgatgc
    15181 gttacgaggt cggcagcgac ggcgcgctgc acaacggcac ggttctccac gacatgaccg
    15241 gcgcgcccgg cgaggacgcg atcgacggcc tgaaggtcga cgaggccggc aacgtctacg
    15301 cctgcgggcc cggcggcatc tgggtgatct cgccggaggg cgagcgactc gggctgatcc
    15361 agctgcccga ggctccgcac aaccttgcgt tcggcgacga cgatcggcgc acgctgtaca
    15421 tcaccgcgct caccggcgtc taccgcctgc gcctaggagt tgctggcgcg cgcccggcgt
    15481 ggcaagcgag aaaggagacc catgagcaag ggactcgaac ccggaatgac gttccccgac
    15541 gtgacgctgc cggacgagaa cggcacgcgc caccggctgt cgacgctcca gggcgacgac
    15601 atgctcgtgc ttcatctcag ccgcggtgag cactgcccgc gcgagcggat gcaccaccgc
    15661 gagctgctgc gcttccacga atggtgctcg gtggcgttca cgcagctcgt gagcatcctg
    15721 ccgaacacgc tgcacgacac gtacaagatg aagatcgcga ccggcgccca ctggaccttc
    15781 ctcgccgacg aggggctcga gctgcgcgaa gcgctcgaga tcgacgagta cacggacaac
    15841 caccacgaca acgcggtggt gccgcacacc ctcatcctct cgcccggcct ccggatcgag
    15901 aaggtctacg tgggctactg gttctggggc cgcccgtcgc cggagcagct ctggctggac
    15961 ctccaggacc tgacgcggcg cgcgaaggcc gactacgacc cgacgctgcc cgacgtgcgg
    16021 gccgagcttc aggtcgccga tgcggcccgc gccgctgcgt a
//
LOCUS       DASVXS010000252        13564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_815386, whole genome shotgun sequence.
ACCESSION   DASVXS010000252 DASVXS010000000
VERSION     DASVXS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13564
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815386"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VF752_14380"
     CDS             <1..608
                     /locus_tag="VF752_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX8856781.1"
                     /translation="AQHGYDVAVYSPDGGKLEGDSWSDPRDESGYSAEDLVSLGFISS
                     PDHVGLVEDTKPIAEIDIDDHDAVLFIGGQGPMVTFRGDERVHRLAASFLAAGKVTAV
                     ICHATCMLLEARDGDGNLLVDGKTWTGFANSEEDYADQFVGERIQPFRIQDEAAKIPD
                     TNFIVAGRFKSHAIRDGNLITGQQQYSARAAAELVIEALGA"
     gene            620..952
                     /locus_tag="VF752_14385"
     CDS             620..952
                     /locus_tag="VF752_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856782.1"
                     /translation="MVMRYWRGWTTPDNADAYERIVSREVLPSIAARDLAGYHGAYLA
                     RRPLGEELEFATVMLFDSIADVRAFAGEDYETAYVPPQAREVLARFDKASAHYDVLMS
                     PDETRLVR"
     gene            949..1236
                     /locus_tag="VF752_14390"
     CDS             949..1236
                     /locus_tag="VF752_14390"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8856783.1"
                     /translation="MSPVTRYVLLYESGDDVAGKAPAHFPAHWARCEEFHERGLLTMV
                     GTFADPQTEGSMAVFTTREGALEFVEGDPFVLNGVVRGWELREWNEAFGEP"
     gene            complement(1253..2047)
                     /locus_tag="VF752_14395"
     CDS             complement(1253..2047)
                     /locus_tag="VF752_14395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8856784.1"
                     /translation="MTVEQRLITTYAAFNTRDIDRVLELMTEDVDWPNAWEGGRVIGH
                     DAVREYWTRQWAELDPEVRPTAFKRLPDGRVEVTVHQIARALDGSVLSDGTVLHTYAF
                     RGDRVARMDVSEPAIELTCESVIHCSAEKIFDVIVDLRGQHRWLERSRSFRGTHEISS
                     DPVTLGTTYREPGPLGVRNGTVTELDRPTTITFHQPMTLRPPIGTIDIVMRYSLEPQA
                     GSTRVRRVVTLQVPRLLKFAMPLLRYEVLRENERTLRALKAYCDSL"
     gene            2124..4904
                     /locus_tag="VF752_14400"
     CDS             2124..4904
                     /locus_tag="VF752_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8856785.1"
                     /translation="MANRRPSLAGTSALRGRRTESALLDNVVAAVRQGESHTLLLLGE
                     AGVGKTALLDYLSEAASDLQVARAVGVESEMELGFAGLHQMCAPMLERVARLPAPQRD
                     ALEIVFGLSEGTAPDPFLVGLGVLSLLSDVADERPLLCVVDDAQWLDQASARTLAFVA
                     RRLRAEPVGLVFAAREAGDALRELPQLELRGLPDQDARALLARAIPGRLDDRVRERIV
                     AETRGNPLALLELPRGASAAELAGGFEIPVGGDLSGRIEDRYLGRVTALPEATQRLML
                     LAAADPAGDAALVWRAARRLGIETSALTPAEDADLLEIGARVRFRHPLVRSAVYRQAP
                     LGARRSTHEALAQESDPELDADRRAWHRALAAAGPDEDVAAELEHSAGRAQTRGGLAA
                     AAAFLERAVALTVDPARRARRALAAAQAKHQAGAPDAALTLLANAQAGPLDPLQRAQG
                     ELLRAQITFTSRRGSNAPPLLLDAAKQLEPLDATLARETYLEALMAAQFAGRLADGAA
                     LKVAEAARAAPPSPTLRAPDLLLDGLAVMITEGHRAAAPFLKGALNVFRAGDIAANGG
                     FRWLWLAEEAAIEMWDHDTWCELAALEIELVRDAGALTVLPLALSANIAAQIFAGELV
                     AAASLIDEVEIAAEATGSRLAPYGSLMLAAWRGREADLAGLTEETLNEVMSRGEGIGV
                     STTQWANALLHNGLGQYGTALGAAEQVLDPPGGRLDATINWALPELIEAAARSGHAER
                     AHDALEQLSEMTGAAGTDWALAVEARSRALLSEHDGAEELYREAIERLGRTRVRGEHA
                     RAHLVYGEWLRREGRRKDARAQLRTAHRMFARMGMGAFAERASRELQATGETVRKRRA
                     ETRDDLTAQEWQIARLARDGLSNPEIGARLFLSPRTVEWHLRKVFSKLGIHSRRELAA
                     ALPVSEPQLTPA"
     gene            complement(4907..5659)
                     /locus_tag="VF752_14405"
     CDS             complement(4907..5659)
                     /locus_tag="VF752_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8856786.1"
                     /translation="MGTLGASPSNNGRGSGAWYQSWTHFRIDRRSPGYCRVTFDHPPI
                     NTITATTVEELAELVGLIEQDADLNVVVFDSANRDFYLAHYDTEHGPARTAALPAGLT
                     GMHAWLDLLVHLSRAPVISIASIRGRVRGAGSEFVLACDLRFASREHAQIGQFDVGTG
                     VVPGSGPTARLPRLVGRGRALEILLVADDFDGPRAEQYGYVNRAIADNELDDEVDDIA
                     SRVARFDHDAIARTKSHVDRVTLAPIASFLDA"
     gene            complement(5698..5895)
                     /locus_tag="VF752_14410"
     CDS             complement(5698..5895)
                     /locus_tag="VF752_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856787.1"
                     /translation="MDIAALAQLLHETAEHHDSFEKAAPPHDWWDWYAAYIDARQSGS
                     TPEEASAAAGRYMAEVKHITV"
     gene            complement(5898..6380)
                     /locus_tag="VF752_14415"
     CDS             complement(5898..6380)
                     /locus_tag="VF752_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856788.1"
                     /translation="MAGTDVSSEPSQPQSGLGVDMKLEVVTLPVSDVERAKSFYQSLG
                     WRLDADIPFSDTVRVVQLTPPHSQCSISFGTGLTAAEPGSAERLELVVSDIEAAREEL
                     IGRGVEVSEPFHLDGGPVPGLDPERRSYNSFATFSDPDGNGWLLQEVTTRLPGREWGD
                     "
     gene            6585..7973
                     /locus_tag="VF752_14420"
     CDS             6585..7973
                     /locus_tag="VF752_14420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8856789.1"
                     /translation="MSSSNDYDVIVLGGGAPGEHCAAALAARGLRVAVVERELVGGEC
                     SYWACIPSKSLLRPGEAVQGAREAGGTAEVDVEAALAWRDFMVSNYSDAGQERWLAGR
                     GIDLLRGTGRLAGTGAVEVDGVRHTAEHIVVATGSDPVVPPISGLRELDGVWGTREAS
                     SMKAVPRRLLVLGGGSAGVEVAQIVRRLGGEAVLVEGADHLLPREPAPLGRELGEALR
                     RDGIELMLGMHATAARREGDEYVLQFDGGTELRGDQLLVATGRRPRVEGIGLETVGVE
                     ADPHGIRVDEYMRVGERLWAIGDVTGIWPLTHVGEYEGDVVAANIAGDAHAANYEAVP
                     RVTYTDPQAAAVGAVEAPYSATAPVSEVPKTATYTHAYAESNGFLTLLSNGERLTGAY
                     ALGPEAGEWMQQATIAIRAHVPLDVLGDTIQPFPSFSGIYDAAVMALRMEVADMPRPV
                     RPMDAQMASLSG"
     gene            7985..8740
                     /locus_tag="VF752_14425"
     CDS             7985..8740
                     /locus_tag="VF752_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8856790.1"
                     /translation="MTATSTTSTQREPALLGQTVVVIGGSAGIGLETARRARAEGADV
                     VLAARDPERLEQAARDVEARSTAAFDATDAAALKRFFGDLPAPIDHVMVTGPGPRYVP
                     LLEMSADDVRQALGDHVVLGLEVARNAAGKMRPGGTLIFMGGTGGRRISRELGIVSAA
                     TAALPPFTAALALELAPVRVNLIAAGFVDTPLSASLLGDRLDARREELRSKLPIRRVV
                     GPADVAALAVHLMINTAVTGATYDIDGGQQFVQ"
     gene            8839..10053
                     /locus_tag="VF752_14430"
     CDS             8839..10053
                     /locus_tag="VF752_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX8856791.1"
                     /translation="MSAATDTAAIRPFQVDIPDEVVDDLRRRIAATRWPEREPVDDQS
                     QGVQLATIQALARYWETEYDFGRLEARLNAFPQFMTEIDGLDIHFIHVRSPQDDALPL
                     IITHGWPGSIVEMLNVVGPLADPTAHGGSAEDAFHVVIPSMPGYGFSAKPDETGWDVP
                     HIARAWAELMNRLGYTRYVAQGGDWGALITEVMGAQAPEGLIGIHTNMPGALPPEVSK
                     VIASNVLGAGDPAPTDLADEEQRSYDRLSFFFTKGIGYALEMGNRPQTLYGLADSPIA
                     LAAWMLDHDIWSLEDISPAFVDGRPVGNLTRDEVLDNVTLYWLTNTGVSSSRLYWENK
                     LGFFDFKGVTVPTAVSAFPNDIFHATRAWAEQAYPNLIYFNGLDRGNHFAAWQEPELF
                     TTEVRTGLRSLR"
     gene            10165..11124
                     /locus_tag="VF752_14435"
     CDS             10165..11124
                     /locus_tag="VF752_14435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856792.1"
                     /translation="MSSSSAPSPTPEGPGSVSGAPNLPAGFTDTFTSRYIDANGIRQH
                     VVIGGDGPPLLLVHGWPQNWYAWRMVMPALARDFEVIAPDQRGTGLTDKPQDGYDPGA
                     LANDMVALMDALGHQRFAMVGTDTGLPIGYALAADHPDRIERVALAEVPGPPGAVPQP
                     PFFVPEPLNNRLWHIAVNRLDDVNEQLVRGREDIYFGYEFGIQAGKKLPDDVINYYVG
                     LLQNPDSLRGSFAFYREWDTMMAQNEQRGKQPLTMPVLGIGGDASNGALVGDAFKPLG
                     NDVRTAVIPGAGHWFAEEAPEETLAALTPFLAPYRDRATAAAS"
     gene            11125..12078
                     /locus_tag="VF752_14440"
     CDS             11125..12078
                     /locus_tag="VF752_14440"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8856793.1"
                     /translation="MTVFGRIHMPSLGGATEWLNSEPLSPEGLRGKGVLVNFWTLTCI
                     NWLRQEPYVRAWSQAYRDDGLVVIGAHTPEFSFEHEIDGVRRATKEREIDYPVAVDND
                     YGVWEAFDNHYWPALYFVDREGIIRDQHFGEGRYEQSEREIQRLLGVERELVSVEGRG
                     VEAEADWDHLRTPETYLGYGRGERFASPDRAAFDERHAYELPERLPLNHWALVGEWTI
                     GSERVVLDQAGGSIAFRFHARDAHLVMSPGEREPIPFRVSIDGEAPGASHGVHVDEDG
                     NGVLDYGRMYQLVREHDAVGERTLEIAFLEPGAEGYAFTFG"
     gene            complement(12149..12844)
                     /locus_tag="VF752_14445"
     CDS             complement(12149..12844)
                     /locus_tag="VF752_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX8856794.1"
                     /translation="MADGLSAAEAAGLARTGASTPSPPRPADHAAELWASLDTFDDAR
                     AQLAFDRLIGEFSVETVLREAVLPYLRMLGERWSLGDASVAQEHFASTLLRERLLALA
                     RGWDAGVGPRAILACPPGERHDLGLVVFGLALRGRGWRVTFFGADTPIDTMAAASAQL
                     QPHAVVLAAVASEPLDAVADGIAALARDHSVFLAGGGADEELAERIGARLLQAGPLEA
                     AAEVGPEPSRARG"
     gene            complement(12818..13006)
                     /locus_tag="VF752_14450"
                     /pseudo
     CDS             complement(12818..13006)
                     /locus_tag="VF752_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            13139..>13564
                     /locus_tag="VF752_14455"
     CDS             13139..>13564
                     /locus_tag="VF752_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX8856795.1"
                     /translation="MYSFTPTRLALVLATTLALSVAVVGPASAASAGPAAKRANIVKT
                     ATDAGQFKTLVSLVKRAGLAGTLAAKSARYTVFAPTDAAFKKVPKRTIDALLKDPAKL
                     RAVLLHHVVKGRVTAKQVVKVRSVQTLNGDSLRLRVRGGK"
BASE COUNT         2156 a         4558 c         4754 g         2096 t
ORIGIN      
        1 tcgcgcagca cggctatgac gtcgccgtct acagcccgga cggcggcaag ctcgagggcg
       61 actcgtggtc cgacccacgc gacgagagcg gctactcggc ggaggacctg gtcagcctcg
      121 ggttcatctc ctcacccgac cacgtgggcc ttgtcgagga cacgaagccg atcgccgaga
      181 tcgacatcga cgatcacgac gccgtgctgt tcatcggggg ccaagggccg atggtcacgt
      241 tccgcggcga cgagcgcgtg caccggctgg cggcgagctt ccttgctgca ggcaaggtca
      301 cggcagtgat ttgccacgcc acgtgcatgc tgctcgaggc gcgcgacggc gacggcaacc
      361 tgctcgttga cggcaagacg tggaccggct tcgccaacag cgaggaggac tacgccgacc
      421 agttcgtggg cgagcggatc cagcccttcc ggattcagga cgaggcagcg aagatcccgg
      481 acaccaattt catcgtcgcg gggcgcttca aatcacatgc gatccgcgac ggcaacctga
      541 tcacgggcca gcagcagtac tccgctcgtg cggctgcgga gctcgtgatc gaggctctcg
      601 gcgcctagga ggcaagcgaa tggtgatgag gtactggcgg gggtggacca ccccggacaa
      661 cgcggacgcg tacgagcgga tcgtgagccg ggaggtgctc ccgagcatcg cggcccgcga
      721 cctcgccggc tatcacggcg cgtatctggc ccgccgtccg ctcggggagg agctcgagtt
      781 cgcgactgtg atgctgttcg actcgatcgc cgacgtccgg gcctttgcgg gcgaggacta
      841 cgagacggcc tacgtgccgc cgcaggcgcg cgaggtgctc gcgcggttcg acaaggcctc
      901 cgcccactac gacgtcctga tgtcgccgga cgagacaagg ctcgtccgat gagccccgtg
      961 acgaggtacg tgctcctcta cgagtcgggc gacgacgtcg ctgggaaggc accggcccac
     1021 ttcccagcgc actgggcgcg ctgcgaggag ttccacgagc gcggactctt gacgatggtc
     1081 ggtacgttcg ccgacccgca gaccgaaggc tcgatggcgg tcttcaccac ccgcgaaggc
     1141 gcgctcgagt tcgtggaggg cgatccgttc gtcctcaatg gcgtggtgcg cggttgggag
     1201 ctgcgcgagt ggaacgaggc cttcggcgag ccgtagccgc tccgctggct tcttacagcg
     1261 agtcgcagta agccttcagg gcgcggaggg tgcgctcgtt ctccctcagg acctcgtacc
     1321 gaagcagcgg catggcgaac ttcagtaagc gcggaacctg aagggtcacg acgcggcgca
     1381 cgcgcgtcga gccggcctgg ggctcgagcg agtagcgcat cacgatgtcg atcgtcccga
     1441 tgggcggcct cagtgtcatc ggctggtgga aggtgatcgt cgtcggccga tcgagctctg
     1501 tcaccgtgcc gttgcgcacg ccgagcggtc cgggctcgcg gtacgtcgta ccgagtgtga
     1561 ccggatccga ggaaatctcg tgagtgccgc ggaacgacct cgacctctcg agccagcggt
     1621 gctgcccgcg gaggtcgacg atcacgtcga agatcttctc ggccgagcag tggataacgg
     1681 actcgcaagt gagttcgatt gccggctcgg atacgtccat gcgtgcgacc cggtcgccgc
     1741 ggaacgcgta cgtgtggagg accgtgccat ccgaaagtac ggacccgtcg agcgcgcgag
     1801 cgatctgatg gacggtcacc tcgacgcgac catcgggcaa ccgcttgaaa gccgtcggcc
     1861 ggacctcagg atcgagttct gcccattggc gcgtccagta ttcgcgcacg gcgtcgtggc
     1921 cgatgacccg tccgccctcc cacgcattcg gccagtcgac gtcctcggtc atcagctcga
     1981 gcacgcggtc gatgtcgcgt gtgttgaagg ccgcgtaggt cgtgatcaat cgctgctcga
     2041 ctgtcatgga tggcgccgcg ggaaagcttt cacatttcct tcatgtgcac ccactcgcgt
     2101 cggtctagga tgcgtgatta ctcatggcca accgccgtcc ctcactggcc gggacgtcgg
     2161 cgttacgagg gcgtcgcacc gagtcggctc tgctagacaa cgtcgttgcg gcggtacgcc
     2221 agggtgaaag ccacacgctc ctgttgctcg gtgaggctgg cgtcgggaag accgcactgc
     2281 tcgactactt gagcgaggcg gcgtcggatc tgcaggttgc ccgtgccgtg ggcgtggaat
     2341 ccgaaatgga gctgggcttc gccgggctgc atcagatgtg cgcgccgatg ctcgagcgcg
     2401 tggcgcggct tccggcaccg cagcgtgacg ccctggagat cgtgtttggc ttgagcgaag
     2461 ggacggcgcc tgatccgttc ctggtgggat tgggggtgct gagcttgttg tctgacgtgg
     2521 cggacgagcg tccgctgctg tgcgtcgtcg atgacgctca atggttggat caggcgtcgg
     2581 ctcgcacgct cgcgttcgtg gcgcggcggc tgcgggcgga gccggtcggg ctcgtattcg
     2641 ccgcgcgcga agctggtgat gcgctccggg agttgccgca gctggagctt cgggggttgc
     2701 ccgaccagga cgcgcgcgcc ctgctggcca gggcgatccc gggccgactc gacgaccgcg
     2761 tccgcgagcg gatcgtggcc gagacgcgag gcaacccgct cgctctgctg gaactgccgc
     2821 ggggagcgag cgcggcggag ctggcaggcg gctttgaaat tcccgtcgga ggagatctct
     2881 cgggccgtat agaggaccgc tatttggggc gggtcactgc gctgcccgag gcgacgcagc
     2941 gactgatgct gctcgcggcg gcggacccag ccggggacgc ggcgcttgtc tggcgtgcgg
     3001 ctcggaggct cggaatcgaa acgagtgccc tcactcccgc ggaggacgcg gatctgctgg
     3061 agatcggggc acgcgttcgg tttcgccatc cgctggtgcg atccgcggtg tatcggcagg
     3121 cgcccctcgg cgcgcggcgg agtacgcacg aggctctggc tcaggagagc gatcctgagc
     3181 ttgacgccga ccgccgtgcc tggcaccggg cgctggcggc ggccggtccc gacgaggacg
     3241 tggccgccga gctcgagcac tcggcagggc gcgcgcagac gcgcggaggc ctcgcggccg
     3301 cagcagcctt cctcgaacga gcggtcgcgt tgacggtcga tcccgcccgt cgggcacgcc
     3361 gtgcgctggc tgccgcgcag gccaagcacc aggccggcgc gcccgacgcc gcgctcacgc
     3421 tgttggccaa cgcgcaggcg ggaccgctcg acccgctgca gcgcgctcag ggagagctgc
     3481 ttcgcgccca gatcaccttc acgtcgaggc gcggaagcaa cgcgcccccg ctcctgctcg
     3541 acgctgccaa gcagctcgag ccgctcgacg cgacgcttgc gcgcgagacc tacttggaag
     3601 cgctcatggc ggcccagttc gccggtcggc tcgccgacgg agccgccctg aaggtcgcag
     3661 aggcggcgcg cgctgcgccc ccgtcgccca cgctgcgcgc ccccgacctg ctcctcgacg
     3721 gcttggccgt gatgatcacc gaagggcacc gcgctgcggc gccgttcttg aagggtgcgc
     3781 tcaacgtgtt ccgtgccggg gacatcgccg cgaacggggg gttccgatgg ctgtggctcg
     3841 ccgaggaagc agcgatagag atgtgggacc acgacacgtg gtgcgagcta gccgcgctcg
     3901 agatcgagct cgtccgcgat gcgggtgctc tgaccgtgct ccctctcgcc ctgagcgcca
     3961 acatcgctgc gcagatattt gccggtgagc tggtcgccgc tgcttcgttg atcgacgagg
     4021 tggagatcgc cgccgaggcg accggcagcc ggctcgcgcc ctacggatcc ctgatgctcg
     4081 ccgcctggcg aggtcgagag gccgaccttg cggggctgac cgaggaaacg ctcaacgagg
     4141 tgatgtcccg gggcgagggg atcggcgtga gcaccactca gtgggcgaac gcgctgctcc
     4201 acaacggact cggtcagtac ggcaccgccc tgggagcggc cgagcaggtg ctcgacccgc
     4261 ccggcgggag gctggacgcg acgatcaact gggcgctgcc cgagctcatc gaggcggccg
     4321 cccgcagcgg tcacgcggag cgtgcccacg acgcgctcga gcaactctcc gagatgacgg
     4381 gcgccgccgg tacggactgg gcgctcgcgg tcgaggcccg ttctcgagca ttgctgagcg
     4441 agcatgacgg ggctgaggag ctctaccggg aggcgatcga gcggctcggc cgcacccgcg
     4501 tacgtgggga gcacgcccgt gcgcatctgg tctacggcga atggctgcgc cgcgagggtc
     4561 ggcgtaagga tgcccgcgcg cagctacgaa cagcgcaccg gatgttcgct cgcatgggga
     4621 tgggggcgtt cgccgagcgc gctagtcgcg agctgcaagc caccggcgag acggtccgca
     4681 aacgccgggc cgagacccgc gacgatctga ccgcgcagga gtggcagatc gcgcggctcg
     4741 ctcgcgatgg cctgtcgaac ccggagatcg gcgcgcggct cttcctcagc ccgcgcaccg
     4801 tggagtggca cctgcgcaag gtgttctcga agctcgggat ccattcgcgt cgggagctcg
     4861 cggccgcact acccgtctcc gagccgcagc tgaccccggc ctgaactcag gcgtcgagga
     4921 aactcgctat cggggccaac gtcacccgat cgacatggga cttcgtgcgc gcgatcgcgt
     4981 cgtgatcgaa acgcgcgacg cgtgaggcga tgtcatccac ctcatcgtcg agctcgttgt
     5041 cggcgatcgc gcggttgacg tacccgtact gctccgcgcg cggcccgtcg aaatcgtcgg
     5101 cgacgaggag gatctcgagc gcccggccgc gacccaccag ccgagggagc cgggccgtcg
     5161 gaccgctccc tggcaccaca cccgttccca cgtcgaactg cccgatctgc gcatgctcgc
     5221 gcgaggcgaa ccgcaggtca caggccagca cgaactcgct tccggcgcca cggacgcgcc
     5281 cgcggataga ggcgatgctg atcacgggcg cgcgggacag gtgcacaaga agatcgagcc
     5341 acgcgtgcat tccggtcaga cctgctggca atgccgcggt cctggccggg ccgtgctccg
     5401 tgtcgtagtg ggcgaggtag aaatcgcggt tggcgctgtc gaagaccacg acattgaggt
     5461 ccgcgtcctg ctcgatcagg ccgacgagct cagcgagctc ttccacggtc gtggctgtga
     5521 ttgtgttgat cggcgggtgg tcgaaggtca cccggcagta gccggggctc cggcgatcga
     5581 tgcggaaatg cgtccaggac tggtaccacg ctccggaacc ccggccgttg ttggaagggc
     5641 tcgcgcccaa ggtgcccatg gctcactcct cacgtgggtc ggtgcgggtc gagcggatca
     5701 gacggtgatg tgcttgacct ccgccatgta gcgcccggcc gccgcggagg cctcctccgg
     5761 ggtgctgccg ctctgccggg cgtcgatgta cgcggcgtac cagtcccacc agtcgtgggg
     5821 tggggcggcc ttctcgaacg agtcgtgatg ctcggccgtt tcgtgcagga gctgtgcaag
     5881 agccgcaatg tccatcgtca gtccccccac tcacggccgg gaagccgcgt tgtgacttcc
     5941 tggagcagcc acccgttgcc atcggggtcg ctgaacgtgg cgaaactgtt gtacgagcga
     6001 cgttccggat ccaggcccgg gacgggccct ccgtcgaggt ggaacggctc gctgacctcg
     6061 acgccgcggc cgatgagctc ctcacgcgcg gcttcgatgt cggagaccac cagctccagg
     6121 cgctccgccg agcccggctc agctgcagtg aggcccgtcc cgaatgagat cgagcactgc
     6181 gagtgcggcg gcgtcagctg gacgacccgg acggtgtcgc tgaaggggat gtcggcgtcc
     6241 aggcgccaac cgaggctctg gtagaagctc tttgcccgct cgacgtcgga gacgggcaac
     6301 gtcaccacct cgagcttcat atccacgccc agaccgctct gcggctggct cggttcgctg
     6361 cttacgtcgg tgccggccat gtggacctcc atcgatggct gtgggcaaca gggtcgcgcg
     6421 tggtggccaa atttcgcgcc cgtggactcc ctggttggaa cactggtcac gcgcggaacc
     6481 agggaccgac cagtgcgtcc acgggcgcga gcggtcgctc ggagcgggac cctgataacg
     6541 gtcgccgcga gcgtggtgcc gccagcaact cgacggaggt agcgatgagc tcgagcaatg
     6601 actacgacgt gatcgttctc ggcggcggcg ctcccggcga gcactgcgcc gcggcgctgg
     6661 ctgcccgcgg actgcgcgtc gccgtcgtcg agcgggagct ggtgggcggc gagtgctcgt
     6721 attgggcttg tattccctcg aagtcactgc tgcgcccagg cgaggcggtg cagggcgcgc
     6781 gcgaggccgg aggcacggcc gaggtcgacg tcgaggctgc gctggcgtgg cgggacttca
     6841 tggtgtcgaa ctattccgac gccggccagg agcgctggct ggcggggcgg ggcatcgacc
     6901 tgctgcgagg cacgggccgg ctggccggca ccggagcggt tgaagtcgac ggtgtccggc
     6961 ataccgcaga gcacatcgtc gtggcgaccg gctcggatcc agtcgtgcct ccgatctctg
     7021 gactccgcga gctcgacggc gtctggggca cccgtgaggc gtcgagcatg aaagctgtcc
     7081 cccgccgcct gctcgtgctg ggtggtgggt cagcaggcgt cgaggttgca cagatcgtgc
     7141 gacgtctcgg cggcgaggcg gtgctcgtcg agggcgccga ccacttgctt ccgcgcgagc
     7201 ctgcgccgct gggcagggag ctcggcgagg ccctgcgccg ggacggaatc gagctgatgc
     7261 tggggatgca cgccacggcc gcgcgacggg aaggcgacga gtatgtcctg cagttcgacg
     7321 gtggcacgga gttgcggggc gatcagctgc tcgtggcaac cggtcggcgc ccgcgcgtgg
     7381 agggaatcgg cctcgaaacc gtcggcgtcg aggccgaccc ccacggcatc cgggttgacg
     7441 agtacatgcg cgtaggcgag cgcctctggg cgatcggtga cgtcaccggc atctggccgc
     7501 tcacccacgt cggcgaatac gagggcgacg tggtcgccgc gaacatcgcc ggcgatgcgc
     7561 atgcggcgaa ctacgaggcc gttccgcgcg tcacctacac cgatccgcag gcggcggcgg
     7621 tgggcgcggt cgaagcgcca tacagcgcca ctgcacccgt gtcagaggtg ccgaagaccg
     7681 ccacctacac gcatgcgtac gccgaatcca acgggttcct cacgctgctc agcaacggcg
     7741 agcgactcac cggcgcctac gcgctcgggc ccgaggccgg tgagtggatg cagcaggcga
     7801 cgatcgccat ccgcgcacac gtcccgctcg acgtgctagg cgacacgatc cagccgttcc
     7861 cgagcttctc ggggatctac gacgccgcag tgatggcgct tcggatggag gtcgccgaca
     7921 tgccgcgacc ggtcaggccg atggacgcgc agatggcgag cctgtcgggg tgaggagaag
     7981 agtcatgact gccacaagca ccacctccac tcagcgcgag ccggcactgc tcggccagac
     8041 cgtagtcgtg atcggtggca gcgccggaat cgggctcgag acggcccgac gcgcccgcgc
     8101 cgagggcgcc gatgtcgtcc tcgccgcccg agatcccgag cggctcgagc aggccgcgcg
     8161 cgacgtagag gcgcggagca ctgccgcctt cgacgccacc gatgcggcgg ctctgaagcg
     8221 atttttcggg gacctgccgg ccccgatcga ccacgtgatg gtcaccggcc ccggaccacg
     8281 ttacgttccg ctgctcgaga tgagcgcaga tgacgtgcgc caagcgctcg gcgatcacgt
     8341 ggtgctgggc ctcgaggtcg cacgcaacgc cgccggcaag atgagacccg gcggcacgct
     8401 gatcttcatg ggcggcaccg gcggccggcg gatcagtcgc gagctcggga tcgtctccgc
     8461 ggccaccgcc gcgctgcccc cgttcacggc ggcactcgcg ctcgagctgg ctccggttcg
     8521 cgtcaacctc atcgccgccg gcttcgtcga caccccgctg tcggcatcgc tgctcggcga
     8581 ccgactcgat gcgcggcgcg aggagctgcg ctcgaagctc ccgatcaggc gagtcgtagg
     8641 accggccgac gtcgccgcgc tcgccgttca cctcatgatc aacaccgccg tcacgggcgc
     8701 gacatacgac atcgacggcg gccagcagtt cgttcagtaa cgggaatctc gaaaccaggg
     8761 agcggaccag ggaccacacg ggcgcgacgt gcggaggaga cgatcaggct tcttgcgtca
     8821 ccgagtacgg gagtccgcat gtctgccgcc acggatacag cagcgatccg gccgttccaa
     8881 gttgacattc cggacgaggt cgtggacgac ctccgtcggc gcatcgcggc cacgcgctgg
     8941 cccgaaaggg agccggtcga cgatcagtcg cagggcgtgc agctcgcgac gattcaggca
     9001 ctcgcgcgct actgggagac cgagtacgac ttcgggcggt tggaggcgag gctgaacgcc
     9061 ttcccgcagt tcatgaccga gatcgacggc ctcgatattc acttcatcca cgtcaggtcg
     9121 ccccaggacg acgcgttgcc tctcatcatc acgcacggct ggcccggctc gatcgtcgag
     9181 atgctgaacg tcgtcggccc gctcgccgac ccgacggcgc acggcggcag cgccgaggac
     9241 gcgttccacg tggttatccc gtcgatgccc ggctacgggt tctccgccaa gccggacgaa
     9301 accggttggg acgtcccgca catcgcgcgc gcctgggcgg agctgatgaa ccgccttggc
     9361 tacacgcgct acgtggccca gggcggcgac tggggtgcgc tgatcaccga agtcatgggc
     9421 gcacaggcgc cggaggggct gatcggcatc cacacgaaca tgcccggcgc ccttccaccc
     9481 gaggtctcga aggtgatcgc gtcgaacgtc ctcggcgcag gcgacccggc cccgaccgat
     9541 ctggcggacg aggagcagcg ctcgtacgac cggctgagct tcttcttcac gaagggcatc
     9601 ggctacgcgc tcgagatggg gaatcgcccc cagacgctgt acgggctcgc ggactcgccc
     9661 atcgccctgg ccgcatggat gctggaccac gacatctgga gcctcgagga catctcgccc
     9721 gccttcgtcg acgggcggcc ggtcggcaac ctgactcgag atgaggttct cgacaacgtc
     9781 acgctctact ggctgacgaa cacgggcgtc tcctcgtccc gcctctactg ggagaacaag
     9841 ctcggcttct tcgacttcaa gggcgtcact gtcccgacgg ccgtcagcgc cttcccgaac
     9901 gacatcttcc acgccacgcg ggcctgggcc gagcaggcgt accccaacct catctacttc
     9961 aacgggctgg acaggggcaa ccacttcgcc gcctggcagg agcctgagct cttcacgacc
    10021 gaggttcgca ccgggttgcg gtcgcttcgc tagcggacca gggagtagac cagggtcctc
    10081 acgggcgcga cgggcagacg agacgagcag cgtgatggcg tcaccacgac caagcgtcgt
    10141 gaggcctcgt gaaaggagat cttcgtgagc tcatcctccg cgccgtcgcc caccccagag
    10201 ggaccaggct ccgtctccgg tgcgccgaac ctcccggccg ggttcaccga cacgttcacg
    10261 agccggtaca tcgacgccaa tgggatccgc cagcacgtgg tcatcggagg tgacggcccg
    10321 cctctgctgc tggtgcacgg ctggccccag aactggtacg cgtggcgcat ggtgatgccg
    10381 gcgctggccc gggacttcga ggtcatcgcg cccgaccagc gcggaaccgg cttgaccgac
    10441 aagccgcagg acgggtacga ccccggcgcc ctcgccaacg acatggtcgc gctgatggac
    10501 gcgctcggtc accagcggtt cgccatggtc ggcactgata ccggattgcc gatcggctac
    10561 gcgctcgccg cggatcaccc agaccgcatc gaacgtgtgg ccctcgctga ggtccccggg
    10621 cctcctgggg cggttcccca accgcccttc ttcgtccccg agccgctcaa caacaggctc
    10681 tggcacatcg ccgtgaaccg gctcgacgac gtgaacgaac agctcgtcag gggacgggag
    10741 gacatctact tcggctacga gttcggcatc caggccggaa agaaactccc ggatgacgtg
    10801 atcaactact acgtcgggct ccttcaaaac ccggactcgc tgcgcggcag cttcgcgttc
    10861 tatcgcgagt gggacaccat gatggcccag aacgagcagc gcggtaaaca gccgctgacg
    10921 atgccggtcc tggggatcgg cggggacgca agcaacggtg cactggtcgg ggacgcgttt
    10981 aagcccctcg ggaacgacgt gcggaccgcg gtcattcccg gcgccggcca ctggttcgcc
    11041 gaggaagccc ccgaggagac gctggcggcg ctgaccccgt tcctggctcc gtaccgcgac
    11101 cgagctacgg ccgccgcgtc ctagatgact gtgttcggac gtatccacat gccctcgctc
    11161 ggcggggcga ccgaatggct caactctgag ccactcagcc ccgaggggtt gcgcggcaag
    11221 ggcgtcctcg tgaacttctg gacgctgacg tgcatcaact ggctgcgcca ggagccctac
    11281 gtccgtgcgt ggtcgcaggc ctaccgagac gacgggctcg tcgtgatcgg agcccacacg
    11341 ccggagttct cgttcgagca cgagatcgac ggcgtgcggc gtgcaacgaa ggagcgagag
    11401 atcgactacc cggtcgcggt cgacaacgac tatggcgtct gggaagcctt cgacaaccac
    11461 tactggccgg cgctctactt cgtcgacagg gaagggatca tccgcgacca gcacttcggc
    11521 gaaggccgct acgagcagtc agagcgtgag attcagcgtc tgctcggcgt cgagcgcgag
    11581 cttgtctccg tcgaagggcg cggcgtggag gcggaggccg actgggacca cctccgtaca
    11641 cccgagacgt acctcggcta cgggcggggg gagcgcttcg cgtcaccgga ccgcgccgcg
    11701 ttcgacgaac gccacgccta cgagctcccc gagcgcctgc ccttgaacca ctgggcgtta
    11761 gtcggagagt ggacgatcgg gagtgagcgt gtcgtgctcg accaggccgg tgggagcatc
    11821 gccttccgct tccacgcgcg cgacgcgcat cttgtgatgt ctcccggcga acgagaaccg
    11881 attcccttcc gcgtgagcat cgacggcgag gctccgggcg cctcgcatgg cgtgcatgtc
    11941 gacgaggacg ggaacggcgt ccttgactac gggcgcatgt accagctcgt gcgcgagcac
    12001 gatgcggtcg gcgaacggac gctggagatc gcgttcctcg agcccggcgc cgagggatat
    12061 gcgttcacgt tcggctagcc ggggaatctc gatcgaggga cgcggtcatg cgcccgcctg
    12121 cccctctccg ccggatcggg gggtcacctc agccgcgtgc gcggctcggc tcggggccca
    12181 cctcggccgc ggcctcgagc ggaccagctt gcaggagccg cgcgccgatg cgctccgcca
    12241 actcctcgtc cgctccccca ccggcgagga agaccgagtg gtcgcgtgcg agggcggcga
    12301 tgccgtcggc caccgcatcc agcggctcgg aggcgacggc cgcgagcacg accgcatgcg
    12361 gttgcagctg ggcgctcgcg gcggccatgg tgtcgatggg cgtgtcggct ccgaagaagg
    12421 tcactcgcca gccacggcct cgaagcgcga ggccgaacac cacgagaccg aggtcgtgcc
    12481 gctcacccgg cggacatgcc aggatcgcgc gcggcccgac cccggcatcc cagccgcgag
    12541 ccagcgcgag cagacgctcg cgaaggagcg ttgacgcgaa gtgctcttgg gccacggacg
    12601 cgtcgcccag gctccagcgc tcgcccagca tccgaaggta gggcagtacc gcctcgcgca
    12661 gcaccgtctc gacgctgaac tcgccgatca gcctgtcgaa ggccagctgg gcgcgtgcgt
    12721 cgtcgaaggt gtcgagggat gcccaaagct cggcggcgtg atcagccggg cgcggcgggc
    12781 tcggagtaga ggcgcccgtg cgtgcaagtc cggccgcttc tgctgcggag agaccgtctg
    12841 ccaggtgtga ctgcatcgcc tgcacacgag cgacgtcggc ggcggagtag agcctgtagc
    12901 cggcctcggt gcgtgtgggc tcgagcaggc cgtagcggcg ctcccacgcc cgaagcgccg
    12961 ccgtagtcac accggcgcgg cgggccacct ccccgattct caacagacct tcggacacgg
    13021 gttgagtata gagcttagac gatctttata cgaacacttg acaaagcacc tttcaccggc
    13081 taccttgtcc aaagcctgtc aagggtttgt tcgtccccgg tctgctcaag gagcacccat
    13141 gtactcattc actccaaccc ggctcgcgct cgtgctggcc accacgctcg ctttgtcggt
    13201 tgcggtcgta gggcccgcca gtgcggcctc ggccggcccg gcggcgaagc gggccaacat
    13261 cgtgaagacg gccacggacg ctgggcagtt caagacgctc gtgtcgctcg tcaagcgcgc
    13321 gggacttgcg ggcacgctcg ccgcgaagag cgccaggtac acggtcttcg ctcccaccga
    13381 tgccgccttc aagaaggtcc cgaagcggac gatcgacgcg ttgctgaagg accccgcgaa
    13441 gcttcgcgcg gttctgctcc accacgtggt gaagggccgc gtgaccgcca agcaggtggt
    13501 gaaggtgcgg tccgtccaga cactgaacgg cgactcgctg cggctccgcg ttcggggcgg
    13561 caag
//
LOCUS       DASVXS010000253        28342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1097031, whole genome shotgun sequence.
ACCESSION   DASVXS010000253 DASVXS010000000
VERSION     DASVXS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 28342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28342
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097031"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VF752_14460"
     CDS             <1..1637
                     /locus_tag="VF752_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8856796.1"
                     /translation="QVQLSIPRVLDVAGASISGTPVVEIGHTHGVAWTHTTSTARRYT
                     LFQLDLVPGRPTSYLVDGRAEPMTRRTLTVTARRAGGKLSRVTRTLYGSRYGPVLATG
                     WTTTTAFAIRDVNADNLRSMNEWLAMGKSQNLAQLRAAQDTYQGNPFTNTIATDAGGR
                     AYFADASVVPHVTDAQAARCINSAEGKARYPDTFVLDGSTSACNWGSDPGAVRRGIFA
                     PNHLPRLIRTDYVANSNQSPWLTNPAAPITGHPAIFGDTGTERELRTRLGLDMIARRL
                     AGTDGLGRPRFTLPTLQATMLANRNYSAELGRADVVAMCRAHPALTASDGTRVDVRAA
                     CDTLARWTGHANAGSRGEALWETFFQAPLTWLVPFDPAHPLTTPRGINGNDPDVQRAL
                     ADAVRLLRSNRTAARARRWAGITLPGCPAEKGCFNVIEASEPITPPPRKPGRRQGQTG
                     PQPSRIGTPASARRQRPSRKPPGIFGSSFIMAVELTSQGPRARTILTHSQSANPASPH
                     YRDQTVLFASRHWVTERFTEAEINADPHLQTTTLHD"
     gene            complement(1938..2705)
                     /locus_tag="VF752_14465"
     CDS             complement(1938..2705)
                     /locus_tag="VF752_14465"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX8856797.1"
                     /translation="MPEHTVGTQQEWQAERNKLLAEEKELTRRSDELARKRRELPWVR
                     VEKEYRFDTDDGTRTLAELFDGRSQLAVYHFMFGPSYETACPTCSSLVDGVNGVLPHL
                     HARDVTMLLVSRAPLAKLQAYKRRMGWSIPWVSSADSDFNFDFGVSYPNELVRERMGP
                     ALDDPQTGLPQIAASTGTDVAGYASEGFGFSAFTLADGAVYYTYSTQGRGAEFLMGYY
                     PILDRAPKGRDEGDARAPASMLWIRRHDEYEDAGATS"
     gene            3040..3630
                     /locus_tag="VF752_14470"
     CDS             3040..3630
                     /locus_tag="VF752_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856798.1"
                     /translation="MTTEHFVLGGARAATIAESNGRANMFLAAVSGGLIALGLVATAS
                     RVGAAFYGFGLVLLPTLAFIGFVTFGRTLQSSIEDTEYARRIAQLRDYYFEHAPEIAP
                     YLVRVPAAERLLMERVPGARWAGYRTVAGMVAVITAVLAGSTAALAAILISDHSLAAG
                     VISATLVTLPTMIVMIRYQDSAWERAAAESLGLSED"
     gene            complement(3884..4558)
                     /locus_tag="VF752_14475"
     CDS             complement(3884..4558)
                     /locus_tag="VF752_14475"
                     /inference="COORDINATES: protein motif:HMM:NF044569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856799.1"
                     /translation="MASSAQTTNLFLLEDLDDPARKGEFENRDLEALQAIAEWINNFV
                     IKPHEDLGRDGTVCPFVPGSLERKVLWLAPERIGDRDPSEVVELMDGYRRLLLDKRSG
                     DGDDAIYDVIVVVFTDLAADRAKAVFDEVQGRLGVPSYAADGNLFGPYYPGNETPAIH
                     NQSFRPFDSPLPVMFVRHGVTGDWESSWTTMSGSSCGQVVTEESAAQALGERLRRLPW
                     RSAGHP"
     gene            complement(5023..5463)
                     /locus_tag="VF752_14480"
     CDS             complement(5023..5463)
                     /locus_tag="VF752_14480"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856800.1"
                     /translation="MSQEDVENLRTWATAWEAPPAGGPMVDCRTGEPVTNHLDLDVVY
                     EDAFLPDHAQETYRGHQGVIRATDRWAEPFEDLRVELERIVDAGACLVSIHRFRARAK
                     HTGIEFDEPLAILWRFRGGRVVYFRSFRDSDDALEAAGLSSSRH"
     gene            complement(5604..6092)
                     /locus_tag="VF752_14485"
     CDS             complement(5604..6092)
                     /locus_tag="VF752_14485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8856801.1"
                     /translation="MSGAEVMEGRGMQIRRDESSTDEVGRAQVEAAKGANGGPPHIHL
                     RQEERFIVHTGELHVRRGRERIRVRAGEDIRIPPKVVHTFQAETESTYTVEFRPALRG
                     WEFFRDLFALPTDRRGNPHVGDAARLMRAYPDEFLYFPFVPVSIQRALAVPLSKLRST
                     LQ"
     gene            complement(6284..6607)
                     /locus_tag="VF752_14490"
     CDS             complement(6284..6607)
                     /locus_tag="VF752_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856802.1"
                     /translation="MQRDVRLVIVVEEDLRFADHRASGLTFCLLDTGELWVSDGERFG
                     PMDALACVLLEETWPDLVIAARDALTARSSRERRPRAKRFIHDKQLETLFARAAADAR
                     TKLAG"
     gene            complement(7020..7211)
                     /locus_tag="VF752_14495"
     CDS             complement(7020..7211)
                     /locus_tag="VF752_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856803.1"
                     /translation="MDSGTRRTRTAAASQDARVPNAEADAVSEYEPPAIEVIDVVAVT
                     LDGIKKEGSGDFGTFKALS"
     gene            complement(7204..8355)
                     /locus_tag="VF752_14500"
     CDS             complement(7204..8355)
                     /locus_tag="VF752_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8856804.1"
                     /translation="MDDQELRTDAVAAPLSELELACGLVFGSEPSATPLPAAGELGSP
                     LVELARPLRRALERPPCLVAFSGGFDSSLVLSVARNVAVRQKLELPIPVTVRFARHLE
                     TQESIWQESVVFELGLSDWERLEVGEELELLGPWARRALHGAGPLWPPNAFMLLPLLD
                     RASGGTLVTGIDGDAALGGSPWRRSAVLLPRAARRAVARRGVPHLGWLTADGRSEMVK
                     AWVRDEGQQPVARDPWLRWYARSRHLAVMRDSFERVASLANARISHPLVDQRVLGAIA
                     KRAGRRGFVTRQGAIDALFEGALPRALAARRGKATFSAPLWGHATRDVAAAMDLSGVP
                     DGFVDRAALRAEWAKLRPDLRSAVLMQHVWLETQTPSGAAEPVIGSVRG"
     gene            complement(8322..10040)
                     /locus_tag="VF752_14505"
     CDS             complement(8322..10040)
                     /locus_tag="VF752_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856805.1"
                     /translation="MTRTARLIAVAVVSTLALSPAVASAKKLRHTTVRAHLIKKQPRA
                     RAHSGATVLGALRDHAPHPRNPGALARAKANAERRFQNGTGQPSPLAAISGGLNLPGL
                     ANSDNLAGAMGTPSDSTGAVGPNNYVEFVNSVIGVYKKSDLTLDSGAGKLGEMDMAAF
                     VGAPGKDVFDPQIQWDPVTQRWYYLIDYIDQDKSGSNPGVNALAFGWSKTADPNVPPS
                     GFNSATGWCRYIVDTGAEFDDYPKLGSNNTHLIFGANVFADNATGGFQTARVFTVNKP
                     GLSTAVGGSNCSTSLTLKSFGTAASNLLTSDGDQAFTPVPAVQTESSTNGWVVAADYA
                     GSGSQSQLMVWHVDSNGDLHADTSSDGNVGVTSYSIPPNIPQPGTNRRIDSSDARITQ
                     AVVRTDPDVGAPAIWTQHTISGGAGAQVRWYEIVPSLCNRTSGTSCNAAARRQQGNLS
                     NASLYYFNGAVSPAGAGNTAVVQYNSGSSAQVTDIRAQSRLSATALGTMTGEVNLGAS
                     SAIAQDFSCPSNGVGSVCRWGDYAAATPDPSASNVVWGSNQLIGTGGMTGGQAVWTTR
                     NFALTP"
     gene            complement(10053..11669)
                     /locus_tag="VF752_14510"
     CDS             complement(10053..11669)
                     /locus_tag="VF752_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856806.1"
                     /translation="MTTDRGAPSDARKRRLVVQKTLIPHRKRYVKFKRRAARHVRRVK
                     RASSPVFGHLANAIGLNAAGLVFSDTPLAWRSTPANATGAVGPNNYVQMTNSAVRVYK
                     KSDLTLDSGAGKLGSMALNDFVGASNREVVYPQVQWDPVTQRWYYLALYVEEDKDATH
                     AGKDALAFGWSKTSDPNVPPSGFNAATGWCRYIVDTGVNFDDYPKLGNNDTRLIIGSN
                     SFTDNSDTGDFQTAHVFTVSKPSAGTACATAPDLKQFGTAASPLKMKNGALATTPVPV
                     YLPQSSANGYVLAADDPGTHGNLIANRIMEWHVDSAGNLHDDTSSNGLFQVSDYKWPG
                     AAAQGGSSNWLDTLDARLTQAVGVTDPTSGVNALALWTQHTILNGTSGAQVRWYELIP
                     SQCNRTGASLCTGGVRQTGNQAPPASTWYFNGAITPTMSGNQAVLIYNSAGNATSTTR
                     STIKADSRLSTDALGAMPQTEQTIATSSDSVEDSSCGAGAGDPCAWGYYAAVAPDPSV
                     TNKAWATSMYTAARIGTTPAWITRNFSFTP"
     gene            11893..12891
                     /locus_tag="VF752_14515"
     CDS             11893..12891
                     /locus_tag="VF752_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8856807.1"
                     /translation="MTEDPFAPATAAGIHRLSIPTPFAVGRVNVYLIEDDPLTLVDAG
                     PNSGTSLEELQHQLGAHGRAIEDIELVVLTHQHIDHLGLVQIVADRSGAEVAAIDAAV
                     PFVENYRDAATDDDAFATEIMVRNGIPEDVVYALRSVSAAFRAWGAPAKVTRVLHDGD
                     RLELRDRTLDVHFRPGHSPTDTIFNDAERRILIAADHLIKNISSNPLISRPPGKQGPR
                     PQSLVTYLESLKKTQAMDVDLVLPGHGDPITDHVALIDERFALHRRRAEKLHGLISEQ
                     PRTAYELAQALWGNIAVTQAYLTLSEVLGHTDILLNEGRVREVEVDGVVRFEAVAE"
     gene            12894..13907
                     /locus_tag="VF752_14520"
     CDS             12894..13907
                     /locus_tag="VF752_14520"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8856808.1"
                     /translation="MESTAPTSEELRIAEQRAEELAERLLPARTPKKRMLVIVNPYAT
                     TVSNRLKNLVVYALRGRYQVDAVDTQAQGHATEITREAAQEGYDVVVAFGGDGTVNEA
                     ANGLVGTPTPLTCLPGGSTNVFCRTLGIPNDVVDATEHLLRMADDFRPRKSDLGRVNE
                     RHFVFASGIGLDASVVERVDRHPYRKAKFGEYYFTWAAVASFTSRYLVNAPKVRVEVD
                     GQSLEGVTVIVQNSDPFTYFGKRPIRIAEGAGIDTGSLAMAVLRRATPIEMPTIIPRV
                     FSGRASTVLRHRQINGFSGVDAARVVSADGRPFPLEVDGDYIGEFEEVAYAVAPRALT
                     VVS"
     gene            complement(13868..14854)
                     /locus_tag="VF752_14525"
     CDS             complement(13868..14854)
                     /locus_tag="VF752_14525"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8856809.1"
                     /translation="MDGTYLVTGCASFIGSHLADDLLAGGARVVGIDSFLPNYASARK
                     RQNLRRALDHDSFELVELDLADPDAELRDLVGDANAVFHLAGEPGVRTSWGSSFESYL
                     RNNVLATQRLLEAAKLWPEKRFVFTSSSSVYGEADELPVTEEAATKPFSPYGATKHAA
                     EELCRVYHENHGVEAVMLRYFSVYGPRQRPDMGFERFCHAALRGEPIRVFGDGRQTRD
                     FTYVSDAIGATRAAAEAPSVAGRVYNIGGDSCVSVNDALTAIARLTYRVLDVHHLERE
                     YGDVRYTAADTSLAREELGLRPSVSLEEGLRRQIEWALAPHETTVSARGATA"
     gene            14913..15311
                     /locus_tag="VF752_14530"
     CDS             14913..15311
                     /locus_tag="VF752_14530"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856810.1"
                     /translation="MAQDNVSVLREAYETLASRGVDAFSEYWTDDISWDATGGSWSGR
                     QAGRRYLQQWYDLFDEFTTEPLEILDAGDDQVVIFIRYSGRSKLTGMKVPPEYFAILS
                     EMRDGKIARAREYATTAEALAAARGGAAPP"
     gene            complement(15308..16066)
                     /gene="arsM"
                     /locus_tag="VF752_14535"
     CDS             complement(15308..16066)
                     /gene="arsM"
                     /locus_tag="VF752_14535"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX8856811.1"
                     /translation="MSPNADIREAVRERYADAARRAAKQDGSGCCGSAAGCGPSSDTA
                     AFGGTLYDEASTEHVPQAAVDASLGCGVPTAVADLHEGETVLDLGSGAGADVLISARR
                     VGPTGKAIGLDMTDEMLELARTNASGAGVDNVEFLKGYLEDIPLPDESVDVVISNCVI
                     NLAGDKHQVLRETARVLRRGGRFAVSDVIADPDMDEATRSDMAAWTGCVAGALTRAEF
                     EEALSAAGLTDIEITETHRVHEHAGSAIIRARKL"
     gene            complement(16063..16587)
                     /locus_tag="VF752_14540"
     CDS             complement(16063..16587)
                     /locus_tag="VF752_14540"
                     /inference="COORDINATES: protein motif:HMM:NF040503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEX8856812.1"
                     /translation="MAGTRLSEATSIRAAERGDLEAVAAIYNQGIADRVATFETQPRQ
                     AADLAEWLAGPGPFVVAERDGQVVGFGRVGAYSDRCVYEGVGEHAVYVDRSARGAGVG
                     TQLLNALSAAAEAAGYYKLSSRAFADNEPSLRIHERAGFERVGVQRRHGKLDGIWKDC
                     VLLERLLGEAADGR"
     gene            complement(16569..16928)
                     /locus_tag="VF752_14545"
     CDS             complement(16569..16928)
                     /locus_tag="VF752_14545"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8856813.1"
                     /translation="MAVDLELVPKQKRPPGEPCCEPVVYPDVEREQAVRMAEVAKALG
                     DPIRLQLVDVLRKHAGKVCVCELVPLFDVAQPTLSHHLKKLRDAGIVDSERRGLWAYY
                     YVIPEALEELSAWLAPA"
     gene            17183..17524
                     /locus_tag="VF752_14550"
     CDS             17183..17524
                     /locus_tag="VF752_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856814.1"
                     /translation="MADGDRTQIARELYRDFASGDRDAVERTLADDFAFSSPLDVGLD
                     RAGYFERCWPGAGRGQKFDFVRLVEAGDEVIVTYELIRSDGTRARNTEVLTFDGDHIC
                     RSEVYFGWNVE"
     gene            complement(17529..18746)
                     /locus_tag="VF752_14555"
     CDS             complement(17529..18746)
                     /locus_tag="VF752_14555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8856815.1"
                     /translation="MPDVDPAPLADGVPSRSVNRRTLLVGAAGALAAAGLRSPARAAG
                     YAASVPARSGPRFDPVLSRRLRRVLLDASRDPSTHFPGAILRVSSPTLGRWTGFVGFG
                     RLNPAEPMRPADRFRAGSIVKTFVAVTVLQLAERGRLSLDDRLTEVLPASVVGRFANA
                     RDITVRMLLSHRSGIPEWDTPAIDDYIAHHPAKVWKVSEFLDLAAAQPPVFAPGTSYR
                     YSNTDYNLLGLIIEHATDHSWRREVTRRVITPLNLPRTYLPAPGHRSLSGAHAHGYGE
                     VDGRTVDLTRVDPSIAGAAGGGALVTTVRDLGRFLDALLEGRLFRSRETLQEMLDFRP
                     APDVGGQVGYGLGIERRVAPGGVDLIGHLGGAAGYAAYVGRLRPRGVTMSYALNWQGD
                     PSPLHFRAINALA"
     gene            complement(18786..19469)
                     /locus_tag="VF752_14560"
     CDS             complement(18786..19469)
                     /locus_tag="VF752_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856816.1"
                     /translation="MEATAPETKKRSPWAIVGASIVVLFSLAVLAAGVAVIWANATQR
                     DSDGYFATKFHRYATPTRAIESQTLDVDAGVPKWIFDRHFAKVRITARSADPSKPVFI
                     GIAKRDALDSYLRSTTHSTIKNLEFNPFSVSFNNVAGRARPEPPASNSIWAAKASGTG
                     TQTVTWKVTGGKWAAAVMNADASPRVAVDGKLGAKVDLFNWLGIVLTAVGGVMTIGAG
                     VLLYLAIRE"
     gene            complement(19541..22012)
                     /locus_tag="VF752_14565"
     CDS             complement(19541..22012)
                     /locus_tag="VF752_14565"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX8856817.1"
                     /translation="MAGSARQTGKDGGSSDRKKAPAKAQQKERTQPKKQAASKRTNGG
                     SAPAKKQPEKVPAAVGAGVTEQGFEHQPVDQAPASKGATLKGLNEFESRLLGDLFAVI
                     EEHSGDHAPAIDRSLVERSFLFACEHHADQRRRSGEGFIVHPVGVAKICAGLRLDTAT
                     LCAALLHDTVEDTSASLEEVKDEFDEEIATLVDGVTKLTGITFQSRDDRQAENYRKMI
                     VAMAADVRVILIKLADRLHNMRTLSALPKQKQQEKAKETLEIFAPLAHRLGIHAIKWE
                     LEDLAFATLHPRKYKEIKGLVSQQRAEREEYVSRAGEYLHKELEAVGINAEISGRAKH
                     FYSIYSKMTRKGREFNEIYDLTAMRVLVESVKDCYGAIGIIHSLWKPLPGRFKDFIAM
                     PKFNMYQALHTTVIGPEGRPLEIQIRTREMHDTAEYGIAAHWIYKDGGYKGGPPQGVE
                     ERVEWLKHLLDWQKELTDPNEFVEGLKGGLFEDEVFVFTPKGEVKSLPAGATPLDFAY
                     EVHTDVGHRCVGAKVNGKIVPLHYELQSGDICEVLTAKQERGPSRDWLSLAKTTRAQS
                     KIRAWFKRERREDSERAGRDILHESLKRQGLPAQKVAGSPLIADVIREMGFKKADDFY
                     IALGQSKISASVVTNKIMQRLKQGESAVEPRGAGDLLQRKETPKPTGSSSGYGIKVEG
                     IDDVMLRLAKCCRPVPGDEIVGYISLGKGITIHHAQCKNARALMKNPERFTKVEWDGA
                     GATSFRAELQVDGWDRTRLLEDLSRTFAESGINIVEARCTVVPPMVKNRFVVEVGDTQ
                     ALKACVSRLRNVDSVFDAYRVTPKG"
     gene            complement(22053..22424)
                     /locus_tag="VF752_14570"
     CDS             complement(22053..22424)
                     /locus_tag="VF752_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856818.1"
                     /translation="MAVKLNNRAYDHAQRLINDGHYVIDERDDWSEHQPSAAQENKFI
                     EEHGYGEYGKWYLGVDDDEREDTKGHWKFPYGDFEAVHRCGVLSAESRAGQRKYIDIE
                     NAAAHLHGMLDALREPQRSRR"
     gene            complement(22476..22868)
                     /locus_tag="VF752_14575"
     CDS             complement(22476..22868)
                     /locus_tag="VF752_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856819.1"
                     /translation="MTTEEISRRMLEAFNAQDLDAMLELYAPDAEVVPVRGALEGIVY
                     RGHEGVRELERESAATWSERRLEDDSVEVRGDKALMLGRLSMRGRASGALTEQRAAFA
                     LHVRDGRIARLATHFDEAAARRELGWDG"
     gene            complement(22873..24798)
                     /locus_tag="VF752_14580"
     CDS             complement(22873..24798)
                     /locus_tag="VF752_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856820.1"
                     /translation="MAIRLHPALLTAAAVLSTLAPAAARAATPIPEGGNAATLPQFEG
                     SPATPEPISAPLPPRHPFMAPNERSNLHVDAYQSDSNPTSGPLGNKTERASTFQGADC
                     ASVSFDQRNRIVTICVGLQGPRLVMMDPRTLDTLATFNLPPRIPTGGGIFTDFSGGGY
                     FYLDNQDRAVLPTTTRHIYVVRETDAPGFALDKDYDVSAAVPFGDSILSAMPDWSGRI
                     WFVSKKGVVGYVDPATGQARSLNTLEPIGNSFAVDETGGVYIVTDKAMYRFDAPAGLP
                     PVATWREPYDNIGQMKPGQSQAGSGTTPTLIGANYVAITDNADPMKIVVYKRAATVAG
                     SRLVCTAPVFQQGSGDTDQSLIGIGNSIVTENNYGYSSPLATENGKTTTPGLERVDIA
                     PDGSACRTVWRSNETAPTVVPKLSLGAGLVYTYTKPPRDDGQDPWYFTALDFDTGKTV
                     FKRLAGEGLGFNNNYAPVTIRPDSSAYVGTLGGLVRIYDTAGPAAARSAGQNAGRSSR
                     GCLPRRGRVTGRRIGRVPLNRSYLGTIRRVGVPTHASATSLRYCVRGGGEIRAAFGNV
                     RARLVATTAKGYRYGRVRVGSRVSRASKRILTGRHAARVSFRVRRGQVRYVVVLDRKL
                     SKHPRQVRTYLTRAGLR"
     gene            complement(24907..25344)
                     /locus_tag="VF752_14585"
     CDS             complement(24907..25344)
                     /locus_tag="VF752_14585"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8856821.1"
                     /translation="MHLEQTFDVRRPRGEVFDYVTNPDKLAEWQTSKTRVEPLTEGPP
                     RQGSRFREFTKPPRGREFEQVTEFSEFDRPHRLHVHIVEGPYPIDGTWTFEPNDGDST
                     TVHFVADGKLTGVLRLLGPIAKRLLNRQFAGYHENLRRNVEAA"
     gene            complement(25388..26932)
                     /locus_tag="VF752_14590"
     CDS             complement(25388..26932)
                     /locus_tag="VF752_14590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8856822.1"
                     /translation="MICYVLFLFPETKGNRMTDTVLQIPPEQQAKSERARWIALWVLC
                     VGMLMIILDATIVNVALPSIQSDLGFSQSSLAWVVNAYLIAFGGLLLLAGRLGDLVGR
                     RRIFLIGLTVFTTASLVCGLAQSQEMLVAARFIQGVGGAMTSAVILGMIVTMFPAPSE
                     QARAIGIYSFVAAAGGSIGLLAGGVLTEAINWHWIFFVNLPIGIATGFLAPRVIERDK
                     GIGIREGADVAGAVLVTGALMLAVYTIVKASDYGWGSARTLGFGAVALGLLAAFVIRE
                     SRARQPLVPLAIFRNRTVSGANLVQTAMVAGLFGMFFLGALYLQRVLHYDAIEVGLAF
                     LPVALGIGILSVGASARLNMRFGARATLLPSLVLILAGLLLFARAPVDGSFLPDILPV
                     MVLLGVGAGLAFPSLMALAMSGVPASESGLASGLVNTSQQVGGALGLAVLATLSTTRT
                     EHLLSSGESNASALTSGFHLAFVVGAGIVAAGVLLAWALLRQPPVAAESDVEPELEAA
                     YAKEAA"
     gene            complement(27026..27538)
                     /locus_tag="VF752_14595"
     CDS             complement(27026..27538)
                     /locus_tag="VF752_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8856823.1"
                     /translation="MGVQTKTIAPVAEPAEPLCDDLCWLLSRASYTLTTELTAAMEHV
                     GLSPRAHAVLSAAMTGEYTQTELAKMVGLDKTTMVVTLDELETAGLAERKQLPTDRRA
                     RVIAVTKSGEKKVREAHEIAEKIRADVLDTLPPREREVFIEALSKLVRERLNEPTPCS
                     QPIRRRAPRA"
     gene            complement(27752..>28342)
                     /locus_tag="VF752_14600"
     CDS             complement(27752..>28342)
                     /locus_tag="VF752_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856824.1"
                     /translation="AGVESVVGGLAREGSRPPGLVSWHALALWPELAAAYDHVVALDP
                     PVVGWGEALLESLPAAGVSAYAHLAWGEAEREFALRVAEYELDLRAGMASLWRSLRDC
                     GGGAAGEELVLLLRGDGRYPRSPLVAARMMRVMCELDLAVLDGSDGGYACRMLEGVRT
                     DLMRSPAARAYGDRLVRIRRLLGVPAPEQPAQPAAA"
BASE COUNT         4322 a         9876 c         9572 g         4572 t
ORIGIN      
        1 atcaggtgca gctatccatt cccagggtgt tggatgtcgc gggcgccagc atctccggca
       61 cgcccgtcgt cgagatcggc cacacccacg gcgtggcctg gacgcacacg acctcgaccg
      121 cccggcgata caccctcttc cagctcgact tggtgcccgg caggccgacc agctacctgg
      181 tcgacggccg cgcagagccg atgacccggc ggacgctgac ggtcaccgcg cgccgtgccg
      241 gtggcaagct ctcgcgggtc acccggaccc tgtatggctc gcggtacggg ccggtgctgg
      301 ccaccggctg gacgaccacc accgcgttcg ccattcgtga tgtcaacgcc gacaacctgc
      361 gctcgatgaa cgaatggctg gcgatgggca aatcacaaaa tctcgcccag ctccgcgccg
      421 cccaagacac ctaccagggc aaccccttca ccaacacgat cgccaccgac gccggcggga
      481 gagcgtactt cgccgacgcc tccgtcgtcc cccacgtcac cgacgcgcaa gccgcacgtt
      541 gcatcaacag cgccgaaggc aaggcccgct accccgacac ctttgtcctg gacggctcca
      601 cgtccgcgtg caactggggc agcgaccccg gcgccgtccg gcgcggcatc ttcgcgccga
      661 accacctccc gcggctgatc cgcaccgatt acgtggccaa ctccaaccag agcccctggc
      721 tcaccaaccc cgccgcgccc atcaccggcc acccggccat cttcggcgac accggcaccg
      781 agcgggagct gcgcacccgg ctcgggctcg acatgatcgc gcggcgcctg gccggcaccg
      841 acggcctcgg ccgaccccgc ttcacgctgc ccactctgca ggccaccatg cttgccaacc
      901 gcaactacag tgccgagctc ggccgcgccg acgtggtcgc catgtgccgc gcccaccccg
      961 ccctgaccgc cagcgacggc acccgggtcg acgtgcgcgc cgcctgcgac accctcgcac
     1021 gctggaccgg ccacgccaac gccggcagcc gcggcgaggc gctctgggag acgttcttcc
     1081 aggctccgct cacctggctg gtgcccttcg atcccgccca cccgctcacc accccccgcg
     1141 ggatcaacgg caacgacccc gacgtacaac gcgccctcgc cgacgcggtg cggttgctcc
     1201 gcagcaaccg caccgccgcc cgcgccaggc gctgggccgg gatcacactg cccggctgcc
     1261 ctgccgagaa gggctgcttc aacgtcatcg aggccagcga gccgatcacg ccgccgccgc
     1321 gcaaacccgg gcgccggcag ggccagaccg gcccgcaacc gagcagaatc gggactccgg
     1381 cgagcgccag acggcagcgc ccgagccgca agccacccgg catcttcggc tcgagcttca
     1441 tcatggccgt cgagctgaca tcccaaggtc cccgcgcacg caccatcctc acccactccc
     1501 aatccgccaa ccccgcctcc cctcactacc gcgaccagac cgtcctgttc gccagcagac
     1561 actgggttac cgagcgattc accgaagccg aaatcaacgc cgacccccac ctacaaacga
     1621 cgaccctgca cgactgacaa ccccggaacc gtcccaccgc tcggttgcgc ccgccagccg
     1681 ccagctcttc tcgcccctgc cagcccgcta cgcggcattc cggggggcat cgaactgttc
     1741 ggccccgtcg gcactcgtcg acgagcgcgc cgatcgtcag gcgcgagcag cggcgcgcga
     1801 agagggatcc ggaccgctgc cccgcggtgg gggctcactg ttccgcgacg ggcgagctcg
     1861 tctcgaggtg ggaggcagca cgagctggaa accaatccct ccacgcggaa gcgaaagcgg
     1921 cgcgggtcat cgggtggtca actcgttgcg ccggcgtcct cgtactcgtc gtgtcgccgg
     1981 atccagagca ttgacgccgg cgcccgcgca tccccctcgt cgcgtccctt cggggcccgg
     2041 tcgaggatcg ggtagtagcc catcaggaac tccgcgcctc gaccctgggt cgagtaggtg
     2101 tagtagacgg ctccatcagc gagggtgaag gcgctgaacc cgaagccctc cgaggcgtat
     2161 cccgccacgt ccgttcccgt cgaggccgcg atctggggca ggcccgtctg cgggtcgtcc
     2221 agcgcagggc ccatccgctc gcggaccagc tcgttggggt acgagacgcc gaagtcgaag
     2281 ttaaagtcgc tgtcggccga ggacacccag ggaatgctcc aacccatcct ccgcttgtag
     2341 gcctgcagct tcgcaagcgg cgcacgcgag acgaggagca tcgtcacgtc gcgcgcatgg
     2401 aggtgcggaa ggacgccgtt gacgccgtcc accagcgatg agcaagtggg gcaggcggtc
     2461 tcgtagctcg ggccgaacat gaagtggtag acggcgagct gggagcgacc gtcgaacagc
     2521 tccgccagtg tccgcgtgcc gtcgtcggtg tcaaacctgt actccttctc gacccgcacc
     2581 cacggcagct cgcggcgctt ccgggccagc tcgtcgctgc ggcgggtgag ctccttctcc
     2641 tccgctagca gcttgtttcg ctcggcctgc cactcctgct gggtcccgac tgtgtgctcc
     2701 ggcatcgcgt tcctcctgtg tcggcctgac cctctggagg gatcttgcca ctcagcggcg
     2761 ccgcccagtg actctcgctg ccgcttccgc gtgccagcga ctagtgaggg cgagaggcat
     2821 tctcggcttc gctctgccct tgggagcgcg agcaccaaca acaccgcggg acccgcgcct
     2881 gccccgcgct cgttgactcg cgcatcctgc ccgagcggtc gtcggttgcg acgataagct
     2941 ccgcgccatc tgggccgcca aggaggaggg gtagccgcga ccaacggcga cgcacgcagc
     3001 accgacgagg gcgacccgcg ctcagcggcg gtcacgtttg tgaccacgga gcacttcgtg
     3061 ctcgggggcg cccgcgccgc gacgatcgcc gaatcgaacg gccgggcgaa catgttcctc
     3121 gcggcggtct ccggcggcct catcgccctg ggcctggtgg cgaccgcaag cagagtggga
     3181 gcagctttct atgggttcgg cctggttctg ctcccgaccc tggccttcat cgggtttgtc
     3241 accttcgggc gaacgctgca gtcctcgatt gaggacaccg agtacgcgcg ccggatcgcc
     3301 cagctgcgcg actactactt cgagcacgcg ccggagatcg ctccgtacct ggtgcgcgtc
     3361 cccgccgcgg agcggctcct tatggagcga gtgcccggcg ctcgctgggc gggataccgg
     3421 acggtcgccg gcatggtcgc ggtgatcacg gcggtgctcg ccggctcgac cgcggccctg
     3481 gcggcgatcc tgatctccga tcactcgctg gccgcgggtg tgatctcagc gacgctggtc
     3541 acgcttccca caatgatcgt gatgatccga taccaggatt cggcctggga acgggccgcg
     3601 gccgagtcgc tcgggctcag cgaggactga ctgaggtgag cgatgagtcg ggggcccgtc
     3661 cgccccgtca agcgggcaac ccgcagcgac tccgtagcgc tgagagcgtg ccgcgctcgc
     3721 tcctccaatg cgcgacgagc agcatcgcga cgtgatcaga gccgaagtgg cgtgccgacc
     3781 gggatcatcg gcgcgcggcg ggcgtggacg gtgtcgatga tctcggcgtt gtcgatccct
     3841 tgaggtagac cgaggtgatg ccgaggttgc tgtggtcgtc gagctatggg tgtcccgcgc
     3901 tgcgccacgg caacctgcgc agtcgctcac caagagcctg cgctgcggac tcctccgtaa
     3961 cgacctgccc acaacttgaa ccactcatcg tcgtccagga agactcccaa tccccggtga
     4021 ccccgtgcct cacgaacata actggcagcg gcgagtcaaa cggccggaag ctctggttgt
     4081 ggatcgcagg cgtctcattg cccgggtagt acggcccgaa gaggttcccg tccgccgcat
     4141 acgacggaac cccaagccgc ccctggacct cgtcgaagac cgccttcgcg cgatccgccg
     4201 ccaaatcggt gaagacgacg acgatgacgt cgtagatcgc gtcgtcgccg tcgcccgacc
     4261 gcttgtcgag caacagtctc ctgtagccat ccatgagctc gacgacctcc gacgggtcac
     4321 ggtcgccaat ccgctccggg gcgagccaaa gcaccttgcg ctccagtgac ccgggcacga
     4381 agggacagac ggtcccgtcg cggccgagat cctcgtgagg cttgatcacg aagttgttga
     4441 tccactcggc gatggcttga agcgcttcga gatcgcggtt ctcgaactcc cccttccgcg
     4501 ctgggtcgtc caggtcttcc agcagaaaga gattcgtggt ctgagccgag ctggccatcg
     4561 aggcctcctt ccggtggacg tgacaccgtc acggtctcat cgcgcctgct cgatcgcacc
     4621 cgtgtcatcc ctggtcagcc cactggcccg cggcggcgac tcgccatccc aatttcccag
     4681 atatccggac cgctgctttc catgtgaggg aacactccgg ccccgagttg cgcttcgcgc
     4741 gacaagtgga agggaccgag cccgcaccgc gtgaccgctg cgggctagcg ctcctcgtcc
     4801 tttgcgggcg tctcgccgag ccgcaacccg acgcctccct tcacgcgcaa ggcggagcgc
     4861 agcttcgctt gcgcgtcgtg tcccctgagc atggagagga gcccgctcgc cctggtgagg
     4921 actccgggaa atcgtccaga gcgccggcga tcaccatcct gacccggcac tcggattccg
     4981 ccgggagtgg cagcggacct cgcgccggtg aatccgaacc ggctaatgcc gactacttga
     5041 cagcccagcc gcttcgagcg catcgtctga atctcgaaag gaccggaagt agacgaccct
     5101 tccgccgcgg aacctccaga ggatcgcgag gggctcatcg aactcgatgc cggtgtgctt
     5161 cgccctcgct cggaagcgat ggatggagac gaggcaagca ccggcatcga cgatccgctc
     5221 caactcgacc ctcaggtcct cgaagggttc tgcccaccga tccgtcgccc ggatcacccc
     5281 ttgatggcct cgataggtct cctgagcatg gtcgggcaga aacgcgtcct catagacgac
     5341 gtcgagatcg aggtgatttg tgacgggctc gcctgtacgg caatcgacca tcgggccccc
     5401 ggcggggggt gcctcccacg ccgtcgccca cgttcgaagg ttttcgacgt cctcctgcga
     5461 catcgcacgt cagtatcgcg tgtgggcccg cttccgatgc gaagtgtgtg ctggcagctg
     5521 cttcgcgcga tgagcgaaag ccgtgtcggt gccgacttcc gcttttcgtc caaagcagca
     5581 cttctgtacc gccgagtcag tgcttactgc agagtggatc tgagcttgga aagcggcacg
     5641 gccagtgctc tctgaatcga cactggaacg aacgggaagt acaggaactc gtccgggtag
     5701 gcgcgcatca ggcgagcggc atcgccgacg tgggggttgc cgcgccgatc ggtcggcagc
     5761 gcgaacagat cgcggaagaa ctcccaaccg cgaagcgcgg ggcgaaactc gaccgtgtag
     5821 gtgctctcgg tctcggcctg aaaggtgtgc acgacctttg gcggaatacg aatgtcctct
     5881 ccggcccgca cgcgtatccg ctcgcggcca cggcgcacgt gaagttcacc ggtgtgaacg
     5941 atgaacctct cttcttggcg aaggtggatg tgcggtggcc cgccgttcgc gcccttcgcc
     6001 gcttcgacct gcgcgcgccc aacctcatcg gtagagctct catcgcgccg gatttgcatc
     6061 ccgcgcccct ccatgacctc agcgccgctc actcagcggc gacgctactc cgccctctac
     6121 tcgaccgcaa cagcggttca gcgacgactg atgccgagaa ctctccttcc agcgaaggag
     6181 cagtgacgcg gcggtccggt tgacgcgctt ggcatgacgc gcgcggcgaa cgggggcgcg
     6241 ccatcgaaat ccgaagtttc ggcgccactg gcaccgggcg cccctagccg gctagtttcg
     6301 ttcgcgcgtc ggcggccgcg cgggcgaaca gcgtttcgag ctgcttgtcg tggatgaagc
     6361 gcttcgcgcg gggacgccgc tcacgcgacg acctcgctgt cagcgcatcc cgcgccgcaa
     6421 ttacgagatc cggccaggtc tcctcaagca gcacgcacgc gagcgcgtcc atcgggccga
     6481 accgctctcc gtcgctgacc catagctcgc ctgtgtccag caggcagaac gtcaggccgc
     6541 tcgcccgatg atcggcaaac cgtaggtcct cctcgaccac gatgaccagc cggacgtcgc
     6601 gctgcaaggc acctcccttg aagagaagtt ggaacccccg cttactacgg gcgtacccaa
     6661 gccgacgacc cagctggcac gtttcttcgc cgcactcagc caacaggcgc ggaaattgcg
     6721 agctacttct ttacgcggag agcgtctgcg gagtgagcca gacgagccgg tgccaaaccc
     6781 tgtgccccag cgcgcccgac tcgggatcag ccgggctgac aagcggtcgc gcggagcggc
     6841 tgagtccgtt gccgaggtga gtggcaaaca tacgccgggc tcctcctttc catgcaacgc
     6901 agcggtcccc agttgactcg gccctagtta cgcggcactc ccgctttaca cgagggagca
     6961 ggagctacgg accgcagatg cgtctcgcgg tgatctcacc ggacgtcccg ggggaagcgc
     7021 tacgacagcg ctttgaaggt cccgaagtcg ccggaacctt ctttcttgat gccgtcgagc
     7081 gtgacggcca cgacgtcgat cacctcgatc gccggcggtt cgtactccga gactgcgtcc
     7141 gcctcggcgt tgggcacacg cgcgtcttgg ctcgcggcgg ccgtgcgagt ccttcgcgtg
     7201 ccgctatcca cggaccgaac ctatcacggg ttcggcggcc ccgctcggtg tttgcgtttc
     7261 aagccagacg tgttgcatca gaaccgcact acgcagatcg gggcgcagct tcgcccattc
     7321 cgctcgcaga gcggcacggt ccacgaaacc gtcaggcacg ccggacagat ccatggcggc
     7381 cgccacgtcg cgagtcgcgt ggccccagag cggtgcgctg aaggttgcct tgccgcgccg
     7441 cgccgcgagc gcacgcggca gcgcgccctc gaacagcgcg tcaatcgcgc cttggcgtgt
     7501 gacgaagccg cgccgcccgg cccgcttcgc gatcgccccg agcacacgtt ggtccacgag
     7561 cgggtgcgag attcgagcgt tcgccagcga agcgacccgc tcgaacgagt cgcgcatcac
     7621 ggcaagatgc cgtgagcggg cgtaccaacg gagccagggg tcgcgcgcga ccggctgctg
     7681 cccctcgtcg cgcacccatg ccttcaccat ctcgcttctc ccgtccgctg tgagccagcc
     7741 gaggtgcggc acaccgcggc gcgcgacggc gcgccgggcc gcgcgcggaa gcagcaccgc
     7801 ggaacgccgc cacggcgagc ccccaagggc cgcgtccccg tcgatgcccg taacgagcgt
     7861 gcctccgctc gcgcggtcga gaagcgggag aagcatgaac gcatttggtg gccagagggg
     7921 gcccgcgccg tggagggcgc ggcgagccca cggcccgagc agctcgagct cctcccccac
     7981 ttcgaggcgc tcccagtcgc tgaggccgag ctcgaacacc acggactctt gccagatgga
     8041 ttcctgcgtc tcgagatgtc gcgcgaagcg aacggtcacc gggatcggca actcgagttt
     8101 ctgtcggacg gccacgttgc gcgcaaccga cagcacgagg gacgagtcga acccacccga
     8161 aaacgcgacc agacaaggcg ggcgctccag agcacgtcgc agcgggcgcg cgagttccac
     8221 gagcgggctg cctagctcac ccgctgctgg gagcggggtg gccgagggct cgcttccgaa
     8281 cacgagcccg caagcgagct cgagttcgct gagcggtgcc gctacggcgt cagtgcgaag
     8341 ttcctggtcg tccatacggc ttggccaccg gtcatgccac cggtccctat gagctggttc
     8401 gatccccaga ccacgttact tgcgctcggg tccggagttg cggccgcata gtcgccccag
     8461 cggcagacgc tgccaactcc gttgctcggg cagctgaagt cttgtgcgat ggcgctgctc
     8521 gcgccgaggt tcacctcgcc cgtcatggtg ccgagcgcag tggcgctgag gcgcgactgt
     8581 gcgcggatgt cggtgacctg ggccgaactg ccgctgttgt actgcacgac tgctgtgttg
     8641 ccggcccccg ccggcgagac cgcgccgttg aagtaataga ggctcgcgtt cgaaaggttg
     8701 ccctgctgac ggcgcgcggc cgcgttgcag ctggtgccgc tcgtgcggtt gcagagcgac
     8761 ggcacgatct cgtaccaacg cacctgcgcg cccgctccgc cgctgatcgt gtgctgcgtc
     8821 cagatcgccg gtgcaccgac gtccggatcg gtgcgcacga ccgcctgcgt gatacgagcg
     8881 tccgagctgt cgatgcgtcg gttcgtgccc ggctgcggga tgttcggtgg aatcgaatag
     8941 ctcgtcacgc ccacgttgcc gtcgctggat gtgtcggcgt gcaggtcacc gttcgagtcg
     9001 acgtgccaga ccatcagttg cgactgcgag cccgagccgg cgtagtcggc cgccacaacc
     9061 cagccgttcg tggagctctc ggtctgcacg gctggtacgg gcgtgaacgc ctggtcgccg
     9121 tcggaggtga gcaggttcga ggcggcggtt ccgaacgact tgagcgtgag gctcgtggag
     9181 cagttggagc cgccgaccgc cgtgctcagg cccggcttgt tgacggtgaa cacgcgcgcc
     9241 gtctggaagc cgcccgtcgc gttgtcggcg aacacgttcg cgccgaagat gaggtgcgtg
     9301 ttgttgctgc cgagcttggg gtagtcgtcg aactcagcgc ccgtgtccac gatgtagcgg
     9361 caccaccccg tcgccgagtt gaagccgctc ggagggacgt tcgggtcggc ggtcttcgac
     9421 catccgaacg ccaacgcgtt cacgcccggg tttgagcccg acttgtcctg gtcgatgtag
     9481 tcgatgaggt agtaccagcg ctgcgtgacg gggtcccact ggatctgcgg atcgaacacg
     9541 tccttgccgg gcgcgccgac gaacgcggcc atgtccatct ctccgagctt gcccgcgccg
     9601 ctgtcgagcg tcaggtcgct cttcttgtag accccgatca cgctgttcac gaactccacg
     9661 tagttgttcg gtccgacggc gcccgtcgag tcggagggcg tgcccatcgc gccggcgagg
     9721 ttgtcgctgt tcgcaaggcc cggcaggttc agaccgccgg agatcgccgc gagcggcgac
     9781 ggctgtccgg tgccgttctg gaagcgacgc tcagcgttag ccttcgcgcg cgcgagcgca
     9841 ccggggttgc gcgggtgcgg cgcgtggtcg cgcagcgcgc cgagcaccgt cgcgccgctg
     9901 tgcgcccggg cgcgcggctg cttcttgatc aggtgcgcgc ggacggtcgt gtgccgcagc
     9961 ttcttcgcgc tcgccacggc gggcgagagc gctagcgtcg acacgacggc gacagcgatc
    10021 agacgggcgg tacgggtcat cggcagctcc atctacgggg tgaacgagaa gttccgcgtg
    10081 atccacgccg gtgtcgtgcc gatgcgcgcc gcggtgtaca tgcttgtggc ccaggccttg
    10141 ttggtgacgc tcggatccgg cgcgacggcg gcgtagtagc cccacgcgca cggatcaccc
    10201 gcaccggcgc cgcagctcga gtcctcgacg ctgtcgctgc tcgtcgcgat cgtctgctcg
    10261 gtctgcggca tcgccccgag ggcgtctgtc gagaggcgcg agtcggcctt gatcgtgctg
    10321 cgcgtcgtgg acgtcgcgtt gccggcgctg ttgtagatga gaacggcctg gttgccgctc
    10381 atcgtcggcg tgatcgcgcc gttgaagtac caggtgctgg cgggcggcgc ttggttgccg
    10441 gtctgccgca cacccccggt gcacagcgac gccccggtgc ggttgcactg gctcggaatg
    10501 agctcgtacc agcgcacctg cgcaccgctc gtgccgttga ggatcgtgtg ctgcgtccag
    10561 agcgcgagtg cgttcacacc cgaggtcggg tcggtgacgc cgaccgcctg cgtgagccgg
    10621 gcatcaagcg tgtcgagcca gttcgaagac ccgccctgcg ccgccgcacc cggccacttg
    10681 tagtcgctca cctggaacag cccgttcgac gacgtgtcgt cgtggagatt gccggcggag
    10741 tcgacgtgcc actccatgat gcggttggcg atgaggttgc cgtgagtgcc ggggtcgtcc
    10801 gccgcgagca cgtatccgtt cgccgagctc tgcggcaggt acaccggcac cggggtcgtg
    10861 gccaacgcgc cgttcttcat cttcagcggc gaggctgcgg tgccgaactg cttcaggtcg
    10921 ggcgccgttg cgcacgccgt gcccgcggac ggcttcgaga ccgtgaagac gtgcgccgtc
    10981 tggaagtcgc ccgtgtcgct gttgtcggtg aacgagttcg agccgatgat cagccgcgtg
    11041 tcgttgttgc cgagcttcgg gtagtcgtcg aagttcacgc ccgtgtcgac gatgtagcgg
    11101 caccagcccg tcgccgcgtt gaagccgctc ggcggtacgt tcggatctga ggtcttcgac
    11161 cagccgaacg cgagcgcgtc cttgcctgcg tgcgtggcgt ccttgtcctc ctcgacgtac
    11221 agcgcgaggt agtaccagcg ctgagtgacc ggatcccact ggacttgcgg atagacgacc
    11281 tcgcggttgc tcgctccgac gaaatcgttg agcgccatcg agccgagctt gccggcgccg
    11341 ctgtcgagcg tgaggtcgct cttcttgtag acgcggacgg cgctgttggt catctgcacg
    11401 tagttgttcg gccccacggc gcccgtggcg ttagcgggcg tactgcgcca ggccagcggc
    11461 gtgtccgaga acaccaggcc cgcggcgttc aacccgatcg cgttcgcgag atggccgaag
    11521 acgggcgagc tcgcccgctt cacccgccgc acgtgccgcg cggcgcggcg cttgaacttc
    11581 acgtagcgct tgcggtgcgg gatgagcgtc ttctgcacca cgagcctgcg cttgcgcgcg
    11641 tccgatggtg cgccccggtc ggtcgtcacc agatgcgcat gcaggatccg cccgaccttc
    11701 accggcttcg cggttcccga ggcaggggcg acgagcgctc ccgtaattag cgcgcacacg
    11761 atcgcaaggg caatgcgagt cctcacaaaa aaatcgcccc gaattcaggt acgtgggccg
    11821 aaatccaccc taagagcgac cctgtcggcg cgcaagctgc gcaacctcgc gtgtatgtag
    11881 ggtgacgcgc tcgtgaccga agacccattc gcgcccgcca ccgcggccgg gatccaccgg
    11941 ctctcgatcc ccacgccctt tgccgtaggg cgcgtgaacg tctacctgat cgaggacgac
    12001 ccactcacgc tcgtcgacgc cgggccgaac tcgggcacgt cgctcgagga gctgcagcac
    12061 cagctcggcg cgcacgggcg cgcgatcgag gacatcgagc tcgtcgtgct gacgcaccag
    12121 cacatcgacc acctcgggct cgtgcagatc gtggccgacc gctcgggagc ggaggtcgcg
    12181 gccatcgacg ccgccgtccc gttcgtcgag aactaccgcg acgcggccac agacgacgac
    12241 gcgttcgcga ccgagatcat ggtccgcaac ggcatccccg aggacgtcgt ttacgcgctc
    12301 cgcagcgtgt cggctgcatt ccgcgcctgg ggagcgccgg ccaaggtcac tcgcgtactg
    12361 cacgacggcg accggctcga gctgcgcgac cgcacgctcg acgtgcactt ccgccccggc
    12421 cacagcccca ccgacacgat cttcaacgac gccgagcgcc gcatcctgat cgccgccgac
    12481 cacctgatca agaacatctc ctcgaatcca ctgatctcgc ggccacccgg caagcagggc
    12541 ccgcgcccgc agtcgctcgt cacctacctc gagtcgctga agaagacgca ggcgatggac
    12601 gtggacctgg tgctgcccgg ccatggcgac ccgatcaccg atcacgtcgc tctcatcgac
    12661 gagcgcttcg cgctgcaccg ccgccgcgcg gagaagctcc acggcctgat ctccgagcag
    12721 ccgcgcaccg cctacgagct cgctcaggcg ctgtggggaa acatcgcggt cacgcaggcg
    12781 tatctcacac tgagcgaggt gcttgggcac accgacatcc tgttgaacga gggccgcgta
    12841 cgcgaggtcg aagtggacgg cgtggtgaga ttcgaggccg tcgccgaatg acgatggaga
    12901 gcaccgcccc caccagcgag gaactgcgga tcgccgagca gcgcgccgag gagctcgccg
    12961 agcgcctgct gcccgcgcgc acgccgaaga agcgcatgct cgtgatcgtg aacccgtacg
    13021 ccacgacggt ctccaatcgc ctgaagaacc tcgtggtcta cgcgctgcgc ggccgctacc
    13081 aagtggacgc cgtcgacacc caggcccagg gccacgccac cgagatcacg cgcgaggcgg
    13141 cgcaggaggg ctacgacgtg gtggtcgcct tcggcggcga cggcacggtg aacgaggcgg
    13201 ccaacgggct cgtcggcacg cccacgcctt tgacctgcct gcccggcggc tccacgaacg
    13261 tcttctgccg cacgctcggc atcccgaacg acgtggtcga cgcgaccgag cacctgctcc
    13321 gcatggccga cgacttccgc ccgcgcaagt cggacctcgg ccgcgtgaac gagcgccact
    13381 tcgtgttcgc ctcgggcatc ggcctcgatg cgagcgtggt cgagcgcgtc gaccggcacc
    13441 cctaccgcaa ggcgaagttc ggcgagtatt acttcacctg ggcggcggtc gcgagcttca
    13501 cgagccgcta cctcgtgaac gcgccgaagg tacgcgtcga ggtggacggt cagtcgctcg
    13561 agggagtgac cgtgatcgtt cagaactcgg acccattcac gtacttcggc aagcggccga
    13621 tccgcatcgc cgagggcgcg ggcatcgaca ccggctcgct cgcgatggcc gtgcttcgcc
    13681 gcgcgacccc gatcgagatg ccgacgatca tcccgcgcgt gttcagcggg cgtgcctcga
    13741 ccgtgctccg gcatcgccag atcaacggct tctcgggagt ggacgccgcg cgcgtggtgt
    13801 cggccgacgg tcggccgttc ccgctggagg tcgacggcga ctacatcggc gagttcgagg
    13861 aagtcgccta cgcggtggcg ccgcgcgcgc tcaccgtggt ctcgtgaggc gcgagcgccc
    13921 actcgatctg gcgccgcagt ccctcctcga gactcacgct cggccggaga ccgagctcct
    13981 cgcgcgcgag tgatgtgtcg gcggcggtgt accgcacgtc gccgtactcg cgttcgaggt
    14041 ggtggacgtc gagcacgcgg taggtgagcc gcgcgatggc cgtgagcgcg tcgttcacgc
    14101 tcacgcacga gtcgccgccg atgttgtaga cgcgcccggc cacgcttggc gcctcggccg
    14161 ccgcgcgtgt ggcgccgatc gcgtccgaca cgtacgtgaa gtcgcgcgtc tggcgaccgt
    14221 cgccgaagac cctgatcggc tcgccgcgca gtgccgcgtg gcaaaagcgc tcgaagccca
    14281 tgtcgggacg ctggcgcggg ccgtagaccg agaagtagcg cagcatcacc gcctccaccc
    14341 cgtggttctc gtggtagacg cggcagagct cctcggcggc gtgcttggtc gcgccgtagg
    14401 gagagaacgg ctttgtggcc gcctcctcgg tcacgggcag ctcgtcggcc tcgccgtaca
    14461 ccgagctcga ggacgtgaac acgaagcgct tctccggcca gagcttggcc gcctcgagca
    14521 gccgctgggt cgcgagaacg ttgttgcgca ggtagctctc gaagctcgag ccccagctcg
    14581 tgcggacgcc gggctcgccg gcgaggtgaa agacggcgtt ggcatcgccc acgaggtcgc
    14641 ggagctcggc gtccggatcg gcgagatcga gctcgacgag ctcaaagctg tcgtggtcga
    14701 gtgctcgcct gaggttctgg cgcttgcgcg cgctcgcgta gttgggcagg aacgagtcga
    14761 tcccgacgac gcgcgcgccg ccggcgagca ggtcgtcggc gagatgggag ccgatgaagc
    14821 tggcgcaacc ggtgaccaga taggtgccgt ccatcctcaa gggaggacgc aatctagcga
    14881 cggttcagtc ccgccgcgat actgcgcgcg ggatggcgca ggacaatgtg agcgttctcc
    14941 gcgaggccta cgaaacgctg gcgagtcgcg gcgtggacgc gttctccgag tactggaccg
    15001 acgacatctc ctgggatgcc accggcggca gctggagcgg caggcaggcc gggcgtcgct
    15061 acctgcagca gtggtacgac ctgttcgatg agttcacgac cgagccgctc gagatcctcg
    15121 acgcgggcga cgaccaggtg gtcatcttca tccgctacag cggccgctcg aagctgacgg
    15181 gcatgaaggt gccgccggag tacttcgcga tcctgagcga gatgcgcgac ggcaagatcg
    15241 cgcgcgcccg cgagtatgcg actaccgccg aggcgcttgc ggcggcgcgc ggcggtgccg
    15301 cgccgcctta gagcttgcgc gcgcggatga tcgccgagcc cgcgtgttcg tgcacgcggt
    15361 gggtctcggt gatctcgata tcggtcaggc ccgccgcgct cagcgcctcc tcgaactccg
    15421 cgcgggtgag ggcgcctgcc acacagccgg tccaggccgc catgtcggaa cgcgtggctt
    15481 cgtccatgtc ggggtcggcg atcacgtcgg agaccgcgaa ccggccgccg cggcgcagca
    15541 cacgcgcggt ttcccgcagc acctggtgct tgtcgcccgc gaggttgatg acgcagttcg
    15601 agatgaccac gtcgacgctc tcgtccggca gcgggatgtc ttccaggtag cccttgagga
    15661 actcgacgtt gtcgacgccg gcgccgctcg cattggtgcg cgcgagctcg agcatctcgt
    15721 cggtcatgtc gagcccgatc gccttgccgg tggggcccac gcggcgggcg gagatcagca
    15781 cgtcggcgcc agcgccggag ccgaggtcga gcaccgtctc gccctcgtgg aggtcggcga
    15841 cggcggtggg cacgccgcag ccgagcgacg cgtcgaccgc ggcttgagga acgtgctcgg
    15901 tgctggcctc gtcgtacagc gtgccgccga acgctgcggt gtcgctggaa gggccacagc
    15961 ccgcggccga accgcaacac cccgagccgt cttgcttcgc cgcgcgccgg gccgcgtcgg
    16021 cgtagcgctc gcgcaccgcc tcgcggatgt cggcgttggg actcatcggc cgtcagcggc
    16081 ctcgccgagc agccgctcga gcagaacgca gtccttccag atcccgtcga gctttccgtg
    16141 acggcgttgg acgccgacgc gctcgaaccc tgcccgctcg tggatgcgca gactcggctc
    16201 gttgtcggcg aaggcgcggc tactcagctt gtagtagccg gcagcttcgg ctgccgcgct
    16261 cagcgcgttc aagagctgag tgcccacccc agcgccacgc gccgagcggt cgacgtagac
    16321 ggcgtgctcg ccgaccccct cgtagacgca gcggtcggag taggcaccga ctcgcccgaa
    16381 cccaacgact tgtccgtcgc gctccgccac cacgaagggc ccagggccgg ccagccactc
    16441 ggcgagatcg gctgcttggc gcggctgcgt ctcgaacgtc gccacgcgat cggcgatgcc
    16501 ctggttgtag atagccgcga ccgcctcgag atcgccgcgc tcggcggcgc ggatcgacgt
    16561 ggcttcgctc aggcgggtgc cagccatgcg ctcaattcct ccagcgcctc cgggatcacg
    16621 tagtagtacg cccagagccc gcggcgctcg gagtcgacga tgcctgcgtc gcgcagcttc
    16681 ttcaagtggt gagacagcgt gggctgcgcg acgtcgaaca gcggaacgag ttcgcagacg
    16741 cagaccttgc cggcgtgctt gcgcagcacg tcgacgagct ggagacgaat cggatcgccg
    16801 agcgccttgg cgacctcggc catgcgcacc gcctgctcgc gctcgacgtc cggatagacg
    16861 acaggctcgc agcagggctc accgggcggc cgtttctgtt tggggacgag ctccaaatcg
    16921 acagccatcg atctacttat atcgattgaa tcgatatcgg tcaatctgat gcactgaggg
    16981 aacacgcgag gtcgcattcg tggggaatcg ccctcgcaga tagcgtcacc cgacgttttg
    17041 cgcgttctgc ttgcgtttcg tagggaatcg gacggcgccg ggacgattac cgacgttttg
    17101 cgccctccca tcccccaccc ggcgatgaga ctcgtcgcac caaatcgtct gacaaccgag
    17161 accaacgaat cgaggaggag ccgtggctga cggagaccga acgcagatcg cacgtgagct
    17221 ctatcgagac ttcgcgtccg gcgaccgcga cgccgtcgag cggacactgg ccgacgactt
    17281 cgcgttctcg agcccgctcg atgtcggact ggaccgcgcc ggctacttcg agcgctgttg
    17341 gcccggcgcg ggccgaggac agaaattcga cttcgtgcgg ctggtggagg ccggggacga
    17401 agtgatcgtg acgtacgagc tcattcggag cgacggcacg agggcccgga acaccgaggt
    17461 gctgaccttc gacggcgacc acatctgccg gtccgaggtc tacttcggct ggaacgtgga
    17521 gtgaccggtc aggcgagcgc gttgatcgcc cggaaatgca gcggcgacgg gtcgccctgc
    17581 cagttgagtg cgtacgacat cgtcacgccc cggggacgga ggcggccgac gtacgcggca
    17641 tagcccgccg cgcctccgag gtggccgatc aaatcgacgc cgccgggcgc gacgcgccgc
    17701 tcgattccga ggccgtaacc gacctgtccc cccacgtcgg gcgccggacg gaaatccagc
    17761 atctcttgca gcgtctcgcg gctgcggaac agccggccct ccagcagcgc atccaggaag
    17821 cggccgaggt cccggaccgt ggtgaccagc gcgccgcccc cggcggcccc ggcgatcgac
    17881 ggatcgacac gcgtcaaatc gacagttctt ccgtcgacct caccgtatcc atgcgcgtga
    17941 gcgccggaca gcgagcggtg ccccggcgcg ggcaggtaag tgcgcggcag gttcagcgga
    18001 gtgatcacgc gccgtgtgac ctcgcgccgc caggagtgat cggtggcgtg ctcgatgatc
    18061 aggccgagca ggttgtagtc ggtgttcgag tatctgtagc tcgtcccggg cgcgaacacc
    18121 ggcggctggg cagccgcgag atcgaggaac tcgctgacct tccacacctt cgcgggatga
    18181 tgcgcgatgt agtcatcgat ggcgggcgtg tcccattccg ggatcccgct gcggtggctg
    18241 agcagcatcc gaaccgtgat gtcgcgggcg ttcgcgaatc gcccgacgac gctcgcgggc
    18301 agcacctcgg tgagccggtc gtcgagggac agccggccgc gctcggcgag ctgcagcacc
    18361 gtgaccgcga cgaacgtctt cacaatgctg ccggcgcgga accggtcggc cgggcgcatc
    18421 ggttcggccg ggttcaagcg accaaagccg acgaagcccg tccacctccc gagcgtcgga
    18481 cttgacacgc ggaggatcgc tccggggaag tgcgtgctcg gatcgcggct cgcgtcgagc
    18541 agcacccgcc ggagccggcg cgacaggacc gggtcgaagc gcggaccgga gcgggccggc
    18601 accgaagcgg cgtaccccgc ggcgcgcgca ggtgagcgga gcccggcggc ggcaagtgca
    18661 ccagcggcgc ccacgagcag cgtcctgcga ttcacggagc ggcttgggac gccgtccgcg
    18721 agcggggcgg ggtcaacgtc cggcatcgcg ttctgctttc ccggcgcctc gatgtccgaa
    18781 ccgcgctact cgcggatcgc gaggtacagg agcacgcccg cgccgatcgt catgacgccg
    18841 ccgacggccg tgagcacgat gccgagccag ttgaagagat cgaccttcgc gccgagcttg
    18901 ccgtccacag cgactcgcgg cgaggcgtcg gcgttcatga ccgcggccgc ccacttgccg
    18961 cccgtcacct tccaggtgac cgtctgagtg ccggtgccgg acgcctttgc ggcccagatg
    19021 ctgttcgagg cgggcggttc cggccgcgct ctgccggcga cgttgttgaa cgacaccgag
    19081 aacgggttga actcgaggtt cttgatcgtc gaatgcgtcg tcgaccggag gtaggagtcg
    19141 agcgcgtcac gcttcgcgat cccgatgaac accggcttcg acgggtccgc cgagcgggcg
    19201 gtgattcgca ccttcgcgaa gtggcggtcg aagatccact tcggcacccc ggcgtccacg
    19261 tcgagcgtct gcgactcgat cgcacgcgtg ggcgtcgcgt agcggtggaa cttggtggcg
    19321 aagtagccat ccgagtcgcg ctgcgtggcg ttggcccaga tgacggcgac gcccgcggcc
    19381 agcacggcca gcgagaacag caccacgatc gacgcgccga cgatggccca cgggctgcgc
    19441 ttcttggtct cgggggcggt tgcttccatg ccgcccagat tgacggccgc ggccccctac
    19501 gacaatggtg ctggctggtg aattcgcgct ctggtgagcg tcagcccttg ggggtgacgc
    19561 ggtaggcgtc gaacacggaa tcgacgttcc gcaggcgtga cacgcacgcc ttcagcgcct
    19621 gggtgtcccc cacctcgacc acgaagcggt tcttcaccat cggcggcacc acggtgcagc
    19681 gcgcctcgac gatgttgatc cccgattccg cgaacgtgcg tgagagatcc tcgagcagac
    19741 gggtgcggtc ccagccgtcc acctgcagct cggcccggaa agacgtcgcg cccgcgccgt
    19801 cccactccac cttcgtgaag cgctccgggt tcttcatcag tgcccgcgcg ttcttgcact
    19861 gcgcgtggtg gatcgtgatc cccttgccga gcgagatgta gccgacgatc tcgtcgccgg
    19921 gcacgggacg gcagcacttc gccaggcgca gcatcacgtc gtcgatcccc tcgaccttga
    19981 tgccgtagcc cgacgagctg ccggttggct tcggcgtctc cttgcgctgc agcaggtcgc
    20041 cggcgccgcg cggttcgacc gcggactcgc cctgcttgag ccgctgcatg atcttgttgg
    20101 tgacgacgct cgcggagatc ttcgactggc cgagcgcgat gtagaagtcg tcggccttct
    20161 tgaagcccat ctcgcggatc acgtccgcga tcagcggcga gcccgccacc ttctgcgccg
    20221 gcaggccctg gcgcttcagc gactcgtgca ggatgtcgcg gccggcacgc tccgagtcct
    20281 cgcggcgctc gcgcttgaac caggcgcgga tcttcgactg cgcgcgcgtg gtcttcgcga
    20341 gcgagagcca gtcgcgcgag ggaccgcgct cctgcttggc ggtgagcacc tcgcagatgt
    20401 cgcccgactg cagctcgtag tggagcggca cgatcttgcc gttcaccttc gcgcccacgc
    20461 agcggtggcc gacgtcggtg tgcacctcgt aggcaaagtc gagcggggtc gcgccggccg
    20521 gcagcgactt cacctcgccc ttcggcgtga acacgaacac ctcgtcctcg aacaggccgc
    20581 ccttcaggcc ctcgacgaac tcgttcgggt cggtgagctc cttctgccag tcgagcaggt
    20641 gcttcagcca ctcgacccgc tcctccacgc cctgcggggg gccgcccttg tagccgccgt
    20701 ccttgtagat ccagtgcgcg gcgattccgt actcggcggt gtcgtgcatc tcacgcgtgc
    20761 ggatctggat ctcgagcggc cgcccctcgg gcccgatcac ggtggtgtgg agcgcctggt
    20821 acatgttgaa cttgggcatc gcgatgaagt ccttgaagcg cccaggcagc ggcttccaga
    20881 gcgagtggat gatcccgatg gcgccgtagc agtccttcac ggactccacg agcaccctca
    20941 tcgcggtgag gtcgtagatc tcgttgaact cgcggccctt gcgagtcatc ttcgagtaga
    21001 tcgagtagaa gtgcttcgcg cggcctgaga tctcggcgtt gatgccgacc gcctcgagct
    21061 ccttgtgcag gtactcgccc gcgcgcgaga cgtactcttc gcgctcggcg cgctgctggc
    21121 tcacgagccc cttgatctcc ttgtacttgc gcgggtggag cgtcgcgaac gcgaggtcct
    21181 cgagctccca cttgatcgcg tggatgccca ggcggtgcgc gagcggcgcg aagatctcga
    21241 gcgtctcctt cgccttctcc tgctgcttct gcttgggcag cgccgacagc gtgcgcatgt
    21301 tgtgcaggcg gtcggcgagc ttgatgagga tgacgcgcac gtcggcggcc atcgccacga
    21361 tcatcttgcg gtagttctcg gcctggcggt cgtcgcggct ctggaaggtg atgccggtga
    21421 gcttcgtgac gccgtcgaca agcgtcgcga tctcctcgtc gaactcgtcc ttgacctctt
    21481 cgagggacgc gcttgtgtcc tcgaccgtgt cgtggagcag cgccgcgcag agggtggcgg
    21541 tgtcgagccg caggccggcg caaatcttcg ccacgcccac ggggtgcacg atgaagccct
    21601 cgccggagcg gcggcgctgg tcggcgtgat gctcacacgc gaacaggaac gagcgctcga
    21661 cgaggctgcg gtcgatcgcc ggcgcgtggt cgcccgagtg ctcctcgatg acggcgaaga
    21721 ggtcgcccag caggcggctc tcgaactcgt tgagtccctt gagcgtggcg cccttgctgg
    21781 cgggcgcctg atcgaccggc tggtgctcga agccctgctc ggttacgccc gcgccgacgg
    21841 ccgccgggac cttctcaggc tgcttcttgg caggcgcgga gcccccgttg gtgcgcttgc
    21901 tggcggcctg cttcttgggc tgtgttcgct ccttctgctg cgccttggca ggggccttct
    21961 tgcggtcgct agagccgccg tccttgcccg tctgccgcgc gcttccggcc acgtattgag
    22021 tctagccctc gacgtggccg aaacggcgcg ggctatcgcc ggcttctctg cggctctctc
    22081 agcgcgtcga gcatgccgtg cagatgcgca gcggcgttct cgatgtcgat gtacttcctc
    22141 tgcccggctc gcgactcggc cgataggact ccacagcggt ggaccgcctc gaagtcgccg
    22201 taggggaact tccagtgccc cttcgtgtcc tcccgctcat cgtcgtccac gccgaggtac
    22261 cacttgccgt actcgccgta gccgtgctcc tcgatgaact tgttctcctg cgcggcgctg
    22321 ggctgatgct cgctccagtc gtcccgctcg tcgatcacgt aatgcccatc gttgatgagc
    22381 cgctgggcgt gatcgtatgc ccggttgttc agcttgactg ccataccgct ggcagtagga
    22441 cgccgggccg cggcgccaag cgcgtggcgc ggtcgtcagc cgtcccagcc gagctctcgc
    22501 cgcgccgccg cctcgtcgaa gtgcgtcgcc aggcgggcga tgcgcccgtc gcgcacgtgc
    22561 agcgcgaacg ccgcccgctg ctcggtgagc gcccctgacg cgcggcctcg catggacagg
    22621 cggccgagca tcagcgcctt gtcgccgcgg acctcgaccg agtcgtcctc gagccgccgc
    22681 tccgaccagg tggccgccga ctcgcgctcg agctcgcgca cgccctcgtg gcctctgtag
    22741 acgatgccct cgagcgcccc gcgcacgggg acgacctcgg cgtcgggcgc gtacagctcg
    22801 agcatggcgt cgaggtcctg cgcgttgaag gcctcgagca tccggcgcga gatctcctcg
    22861 gtggtcacgc ggtcagcgta agccagcccg cgtgaggtat gtacggacct ggcgcgggtg
    22921 cttgctcagt ttccggtcca agaccaccac ataacgcacc tggccgcggc gcacgcggaa
    22981 cgagactcgg gccgcgtggc gcccggtgag gatgcgtttt gacgcgcggc tgacccggct
    23041 gccgacccgg acacggccgt agcggtagcc cttggcggtc gtcgcgacga gccgcgcccg
    23101 gacgttgccg aacgccgcgc ggatctcgcc gccgccgcgc acgcagtagc gcaggctcgt
    23161 ggccgacgcg tgcgtgggca cgcccacgcg gcggatcgtg ccgaggtatg agcggttcaa
    23221 ggggacgcga ccgatccggc ggccggtcac gcgccctcgc ctcggcaggc agccgcgcga
    23281 cgagcggccc gcgttctggc ccgcggacct ggcggccgcc ggccccgccg tgtcgtagat
    23341 gcgcacgagc ccgccgagcg tgcccacgta ggcggagctg tcggggcgga tcgtgaccgg
    23401 cgcgtagttg ttgttgaagc cgagcccctc ccccgcaaga cgcttgaaca ccgtcttgcc
    23461 ggtgtcgaag tcgagcgcag tgaagtacca ggggtcctgc ccgtcgtcgc gcgggggctt
    23521 cgtgtacgtg tagacgaggc cagcgccgag tgagagcttc ggcaccacgg tcggcgcggt
    23581 ctcgttgctt cgccagaccg tgcggcaggc gctgccgtcg ggcgcgatgt ccacgcgctc
    23641 gagcccgggc gtcgtggtct tcccgttctc tgtcgcgagc gggctcgagt agccgtagtt
    23701 gttctcggtg acgatcgagt tgccgattcc gatcagcgac tggtcggtgt cgccggaccc
    23761 ctgctggaag acgggcgcgg tgcagacgag gcggctgccg gcgactgtcg cggcccgctt
    23821 gtagacgacg atcttcatcg gatcggcgtt gtcggtgatc gccacgtagt tcgcgccgat
    23881 cagcgtgggc gtcgtgcccg agccggcctg gctctggcct ggcttcatct ggccgatgtt
    23941 gtcgtagggc tcgcgccagg tcgcgaccgg cggcaggccc gcgggcgcgt cgaagcggta
    24001 catcgccttg tcggtgacga tgtacacgcc gccagtctcg tcgacggcga acgagttgcc
    24061 gatcggctcg agcgtgttga gcgagcgtgc ctggccggtc gcgggatcca cgtagccgac
    24121 cacgcccttc ttggacacga accagatgcg cccggaccaa tcgggcatcg cggagaggat
    24181 gctgtcgccg aacgggacgg ccgcgctcac gtcgtagtcc ttgtcgagcg cgaagcctgg
    24241 cgcgtcggtc tcgcggacca cgtagatgtg gcgcgtggtg gtgggcagca ccgcgcgatc
    24301 ctggttgtcg aggtagaagt agccgccgcc cgagaagtcg gtgaagatcc cgccgcccgt
    24361 gggtatgcgc ggcggcaggt tgaaggtggc gagcgtgtcg agcgtgcgcg ggtccatcat
    24421 cacgaggcgc ggtccctgca gacccacgca gatcgtcacg atgcggttgc gctggtcgaa
    24481 cgagacgctt gcgcagtcgg cgccctggaa ggtcgaggcc cgctcggtct tgttgccgag
    24541 cggcccgctc gtggggttcg agtcgctctg gtaggcgtcg acgtggaggt tgctgcgctc
    24601 gttgggcgcc atgaaggggt ggcgcggagg cagtggagcg ctgatcggct cgggggtcgc
    24661 cgggctgccc tcgaactggg gcagcgtggc ggcgttgccg ccttcgggga tcggcgtggc
    24721 cgcgcgggct gcggcgggcg cgagcgtact cagtactgcc gccgcggtga gcagcgctgg
    24781 gtgtagccgg atcgccatcg gactgcctcc ggcgcgaata ctgccaagcg gggcgcggcc
    24841 tcgcgccgcc ccggccgctg ggggctcgac tgacggcggc tctctttttt cagcgttgcg
    24901 cgccgctcag gcggcctcga cgttgcggcg caagttctcg tggtagccgg cgaactgccg
    24961 gttgagcagc cgcttcgcga tcgggccgag cagccgcagt acgccggtca gcttgccgtc
    25021 ggcgacgaag tgcacggtgg tcgaatcgcc gtcgttcggt tcgaacgtcc aggtgccgtc
    25081 gatcgggtac gggccctcga cgatgtgtac gtgcaggcgg tgcggccggt cgaactcgct
    25141 gaactctgtg acctgctcga actccctccc gcgcggtggc ttcgtgaact cgcggaagcg
    25201 cgagccctgc cgcggcggtc cctccgtaag cggctcgacg cgggtcttcg acgtctgcca
    25261 ctcggcgagc ttgtccgggt tcgtgacgta gtcgaagacc tccccgcgcg gccgtctcac
    25321 gtcgaaggtc tgctcgaggt gcatcccggg cgcagcctac ctctcggcag gccgcgcccg
    25381 ggtgtgacta cgcagcctct ttggcgtatg ccgcctcgag ctccggctcg acgtcggact
    25441 ccgccgcgac aggcggctgc cggagcagcg cccacgcgag gaggacgccc gcggcgacga
    25501 tgcccgctcc caccacgaac gcgaggtgga agccggacgt gagcgccgac gcgttcgact
    25561 cgccgctcga gagcaggtgc tccgtgcgcg tggtcgacag cgtggcgagc acggccaggc
    25621 cgagcgcgcc gcccacctgc tggctcgtgt tgacgaggcc ggaggcgagg ccggattcgc
    25681 tcgccgggac gcccgacatc gcgagcgcca tcagggacgg gaacgcgagc cccgcgccga
    25741 ctccgagcag caccatcacc ggcaggatgt ccggcaggaa gctgccgtcg accggcgccc
    25801 gcgcgaacag cagcaggccg gcgaggatca gcaccaggct cggcaggagc gtcgcccgtg
    25861 cgccgaagcg catgttcagc cgcgccgacg caccgaccga caggatgccg attccgagcg
    25921 ccaccggcag gaacgcaagc cccacctcga tcgcgtcgta atgcagcact cgctgcaggt
    25981 agagcgcgcc gaggaagaac atgccgaaca ggccggcgac catcgccgtt tgcacgaggt
    26041 tcgccccgct gaccgtccgg ttgcggaaga tcgcgagcgg cacgagcggc tggcgtgcgc
    26101 gcgactcgcg gatcacgaag gccgcgagca gcccgagcgc caccgcgccg aaaccgagcg
    26161 tgcgcgccga gccccagccg tagtcgctcg ccttcacgat cgtgtagacc gccagcatca
    26221 gcgcaccggt gacgagcacg gcgccggcga cgtcggcgcc ctcgcgaatg ccgatgccct
    26281 tatcgcgctc gatcactctc ggcgcgagga acccggtcgc gatcccgatc gggaggttca
    26341 cgaagaagat ccagtgccag ttgatggctt cggtcagcac gccgccggcc agaaggccga
    26401 tcgaaccgcc ggccgccgcc acgaagctgt atatgccgat tgcgcgcgcc tgctcagacg
    26461 gcgcggggaa catcgtcacg atcattccga ggatgaccgc ggaggtcatc gcgcccccga
    26521 cgccctggat aaagcgcgcc gccacgagca tctcctggct ctgggcgagc ccgcacacga
    26581 gcgaggccgt cgtgaagacg gtcaggccaa tcaggaatat gcgccgccgt ccgaccaggt
    26641 cgccgaggcg gccggcgagc agcagcagtc cgccgaacgc gatcaggtac gcgttcacga
    26701 cccaggcgag gctcgactgc gagaagccga gatcgctctg aatcgacggg agggcgacgt
    26761 tcacgatcgt cgcgtcgagg atgatcatca gcattccgac gcagagcacc cagagcgcga
    26821 tccagcgcgc ccgctcgctc ttcgcctgct gttccggggg gatctggaga accgtgtccg
    26881 tcatgcggtt gcccttggtc tcggggaaga gaaataaaac gtagcagatc atcccgttcg
    26941 ggatcttttc gtccggaact atgtgcggta tgtcactgag cgcgccgctc ggcggcggtc
    27001 gttgggcccg gtctggagag cgcgcttaag cgcgcggggc gcgccggcgg atcggctgcg
    27061 agcagggcgt cggctcgttg agccgctcgc gaacgagctt gctgagcgcc tcgatgaaga
    27121 cctcgcgctc gcgtggcgga agcgtgtcga gcacgtcggc gcggatcttc tcggcgatct
    27181 cgtgcgcctc gcggaccttc ttctcccccg acttcgtgac cgcgatgacg cgcgcacgcc
    27241 gatcggtcgg caactgcttg cgctcggcga ggccggcggt ctcgagctcg tcgagggtga
    27301 ccaccatcgt ggtcttgtcg agaccgacca tcttggccag ctcggtctgg gtgtactcgc
    27361 cggtcatcgc ggcactgagc acggcgtgcg ctcgcggcga gaggcccaca tgctccatcg
    27421 ccgcggtcag ctccgtcgtg agcgtgtagc tggctcgcga gagcagccag cagaggtcgt
    27481 cacagagcgg ctcggcgggc tcggctactg gggcgatggt cttggtctgg acacccattt
    27541 tcacaattca tcctagtcga tggtttcgct gatggaccat cttctacgga actatcgccg
    27601 ccgggggcgg cgaggtttgt tccttggtcg ttgcatggtt gccaaggagc tctgaagagc
    27661 gaggcgctat cgcggccgct tgcaactcct gagttttttg ccggtatgcg gcaagaaagt
    27721 caggagtagt gctcaggttg gttctctgcc gctacgccgc ggccggctgc gccggttgct
    27781 ccggcgccgg cacgccgagc agacgacgga tgcgcaccag gcggtcgccg tacgcgcgcg
    27841 ctgccggcga ccgcatcagg tccgtgcgca cgccctcgag catccgacac gcgtagccgc
    27901 cgtcactgcc atcgagaacc gccaggtcca gctcgcacat cactcgcatc atcctcgccg
    27961 caacgagcgg cgaacgcggg tagcggccgt caccgcggag caacagcacc agctcctcgc
    28021 cggcagcacc cccgccgcag tcccgcagcg accgccacag cgacgccatc cctgcgcgca
    28081 gatccagctc gtactcggcc actcgtagcg cgaactcgcg ctccgcctcg ccccaggcca
    28141 ggtgcgcgta cgcgctcact cccgcggccg ggagcgactc gagaagggcc tcgccccatc
    28201 cgacgaccgg cggatcgagc gcgaccacgt ggtcgtacgc cgcggcgagc tcaggccaca
    28261 acgcgagcgc gtgccacgac acgagcccgg gcggccgcga gccctcccgt gcgagcccac
    28321 ccaccacgct ttccacccct gc
//
LOCUS       DASVXS010000254         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_746580, whole genome shotgun sequence.
ACCESSION   DASVXS010000254 DASVXS010000000
VERSION     DASVXS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746580"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="dxr"
                     /locus_tag="VF752_14605"
     CDS             <1..1039
                     /gene="dxr"
                     /locus_tag="VF752_14605"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX8856825.1"
                     /translation="EQLLEQARRHGVERIALADERAAARAAESWTEGEVLTGAEGLVG
                     LITDTDCDLVLNALVGSVGLGPTIATLGEGIDLALANKESLVVGGELVMALAEATGSS
                     VIPVDSEHSALKQLIDSERPGTVDRLVLTASGGPFRGRSAAELADVTVEQALAHPTWD
                     MGGKITIDSATLMNKGLELIEAHHLFGVGYDRIDIVVHPQSIIHALVHLNDGASLAHM
                     GYPDMRVPISYALHHPDRADVPVPTLDLPAVGQLTFEEPDTEAFPCLRLAREAAEAGG
                     TAPCVLNAANEVAVHAFLGGRLTFPGIAEVIERSLEQLPAEPVMHFSDLYRADAAARA
                     RATELVGAVRA"
     gene            1036..2112
                     /locus_tag="VF752_14610"
     CDS             1036..2112
                     /locus_tag="VF752_14610"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8856826.1"
                     /translation="MSWLLAFIGFAVLVILHEFGHFTAAKAVGMRVERFSLFFPPFLW
                     RKRRGETEYAIGALPLGGYVKISGMNPDEDLPDEVRTRAYYSQPVWKRVVVIAAGPGV
                     NVVLAFLLLFVFFWQIGGTDVSNQIDTVNKGFPASGQLHHGDRLIAVDGKRAKPDQLS
                     TIIKGYHCAGGATVQGCKATKPATLTLERDGKVTQLKLTPVYDAQLKRMRLGFSFDQV
                     HRTLPAGNAASTTIDRMWFLTKETVSLPARLLNPEKRKEISGVVGGYEVTRQTIATDA
                     GDAVAILALISLSLAIINLFPFLPLDGGHIFWAVVEKIRRKPVPYSVMERAGVIGFAL
                     VLGLFVIGLSNDIGRLTGQGFGVR"
     gene            2125..2892
                     /locus_tag="VF752_14615"
     CDS             2125..2892
                     /locus_tag="VF752_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX8856827.1"
                     /translation="MTEPFLLVQLSDPHIGATWVDADPVARLAAAVEAVRAMRPAADA
                     VIISGDLADHAAEAEYERLRALISPLEAPIYVLPGNHDDRAALRHYFEVPRGAGEPIQ
                     YAVDLGPLRLVVLDTTAPGQDSGVLDAERLAWLDSELATASDKPTLVAMHHPPLHAGL
                     PALDEIGLAVADRRALGEVIERHPQVRRLVAGHMHRTISGALAGRTVLAAPSTYVQAR
                     LDFSSHDLQFTTEPPAFAVHAVLDGEVLSHVRVVEPT"
     gene            2957..3709
                     /locus_tag="VF752_14620"
     CDS             2957..3709
                     /locus_tag="VF752_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856828.1"
                     /translation="MRISITAVLIAALAGGAWSSSSSGSSAEKSPSELAPVHGRYSPS
                     IDARNFVAKVDNRYWPLEPGTAFHYEGVRGRTRQTDDEIVTHATKRILGVKCTVVRDT
                     VSEHGRPVERTYDWYAQDKQGNVWYMGELSLERRHGRFVKASDSWEAGVKGAKPGIIM
                     PANPKPGDRYRQEYYPPGQALDEAHVLRQRPTVTVPYGSFKRVLVTSEYSPLEPQTEH
                     KYYVAGLGEVQETVVKGHHEEFKLVSVTKPSG"
     gene            3734..4477
                     /locus_tag="VF752_14625"
     CDS             3734..4477
                     /locus_tag="VF752_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856829.1"
                     /translation="MRRPLLFVTLLATVAACAAGCGGGESRRPSGTTHTSTAPAQAAA
                     PEPVDGSDVSFRASDGKTVKGVLTSAGRRAPAVILVHEVNGGADQWDQFVPYLHEAGF
                     TTLAYDGRGGLDEVDLAREVAGAISFLGKRPEADPKRIQVVGASIGASTAVMSIATNG
                     RRSLAGAVALSPADSPSFIELQAKDRYAPHDVLFVSDTREAGNLQNLVPGAVRSKKLE
                     SDDPGHGVALLQHAEVRDAVLAWLKQHAA"
BASE COUNT          716 a         1574 c         1559 g          688 t
ORIGIN      
        1 cgagcagctg ctggagcagg cgcgccggca cggggtcgag cggatcgccc tcgccgacga
       61 gcgcgccgcg gcgcgcgcgg cggagtcctg gaccgagggc gaggtgctca cgggcgccga
      121 gggccttgtc ggcttgatca ccgacacgga ctgcgatctc gttctcaacg cgctcgtcgg
      181 ctcggtgggc ctcgggccca cgatcgcgac gctcggcgag gggatcgacc tggcactcgc
      241 caacaaggag agcctcgtgg tcggcggcga gctggtgatg gcgctcgccg aggccaccgg
      301 atcgagcgtg atcccggtcg actccgagca ctccgcgctc aagcagctga tcgattccga
      361 gcgccccggc acggtcgacc ggctggtgct cacggcttcg ggcggtcctt tcaggggccg
      421 cagcgccgcc gagctcgccg acgtgaccgt cgagcaggcg ctcgcgcatc ccacctggga
      481 catgggaggg aagatcacga tcgactcggc cacgctgatg aacaaggggc ttgagctgat
      541 cgaggcgcac cacctgttcg gcgtcggcta cgaccgcatc gacatcgtcg tgcacccgca
      601 gtcgatcatc cacgcacttg tgcacctgaa cgacggtgcc tcgctcgcgc acatggggta
      661 tccggacatg cgagtgccga tctcctacgc cctgcatcac cccgaccgcg ccgacgtgcc
      721 cgtgccgacg ctcgacctgc cagcagtcgg tcagctgacg ttcgaggagc ccgacaccga
      781 agcgttcccc tgcctgcgcc tggcgcgcga ggcggcggag gcgggcggca cggctccctg
      841 cgtgctgaac gcggcgaacg aggtggcggt gcacgccttc ctcggcgggc gcctcacctt
      901 tcccggaatc gccgaggtga tcgagcgctc gctcgagcag ctgccggccg agccggtgat
      961 gcacttcagc gacctctacc gcgccgacgc tgccgcgcga gcgcgcgcga ctgagttggt
     1021 gggggcggtg cgcgcgtgag ctggctgctc gccttcatcg gcttcgcggt cctcgtgatc
     1081 ctgcacgagt tcggtcactt caccgctgca aaggcggtcg gcatgcgggt ggagcgcttc
     1141 tcgctcttct tcccgccgtt cctgtggcgg aagcgaaggg gggagacgga gtacgcgatc
     1201 ggggcgctcc cgctcggggg ctacgtgaag atcagcggca tgaaccccga cgaggacctc
     1261 cccgacgagg tccgcacgcg ggcgtactac tcgcagccgg tgtggaaacg cgtcgtggtg
     1321 atcgccgccg gcccgggggt gaacgtggtt ctcgcgttcc tgctgctgtt cgtcttcttc
     1381 tggcagatcg gcggcaccga cgtctcgaac cagatcgaca cggtgaacaa gggcttcccg
     1441 gcttccggcc agctccatca cggcgacagg ctgatcgccg tggacggcaa gcgggcgaag
     1501 ccggatcagc tctccacgat catcaagggc taccactgcg cgggcggagc gacggtgcag
     1561 gggtgtaagg ccacgaagcc cgcgacgctg acgctcgagc gcgacggcaa ggtgacccag
     1621 ctgaagctga cgccggtgta cgacgcgcag ctgaagcgta tgcgcctcgg cttctcgttc
     1681 gaccaggtcc atcgcacgct ccccgccggc aacgcggcca gcacgacgat cgaccgcatg
     1741 tggttcctca ccaaggagac cgtgagcctg ccggcgcgcc tgctgaaccc ggagaagcgc
     1801 aaggagatct cgggcgtggt gggcggctac gaggtcacgc gccagacgat cgccaccgac
     1861 gctggcgacg cggtcgcgat cctcgccctg atctcgctct cgctcgcgat catcaacctg
     1921 ttcccgttcc tgccgctcga cggcggccac atcttctggg cagtcgtcga gaagatcagg
     1981 cgcaagccgg tgccctacag cgtcatggag cgcgcggggg tgatcggctt cgcgctcgtg
     2041 ctgggactct tcgtgatcgg cttgtcgaac gacatcggca ggctcacggg acagggcttc
     2101 ggcgttcgct agcgcctctt cgcgttgacc gagccgtttc tcctcgtcca gctgagcgac
     2161 ccgcacatcg gcgccacttg ggtggacgca gatcccgtcg ccagactcgc cgccgcggtc
     2221 gaggcggtac gcgcaatgcg gcccgccgcg gatgcggtga tcatctccgg tgacctggcg
     2281 gaccacgccg cggaggcgga gtacgagcgg ctccgcgcgt tgatctctcc gctcgaggca
     2341 ccgatctacg tgctccctgg caaccacgac gatcgcgcgg cgctgcggca ctacttcgag
     2401 gtgccgcgtg gcgcgggtga gccgatccag tacgcggtcg acctcggacc gctgcggctg
     2461 gtggtgctcg acacgacggc gcccgggcag gacagcggcg tgctcgacgc ggagcggctt
     2521 gcgtggctcg actccgagct tgccaccgcg tccgacaagc ccacgctggt cgcgatgcac
     2581 cacccgccgc tccatgccgg cctgccggcg ctcgacgaga tcggcctcgc agtcgccgac
     2641 cgccgcgcgc tcggcgaggt catcgagcgc cacccgcagg tgcgccgcct cgtcgccggt
     2701 catatgcacc gcaccatttc gggcgctctt gcgggacgca cggtgctcgc agcgccgagc
     2761 acttacgtac aggcgcggct cgacttcagc tcgcacgatc tccagttcac gacggagccg
     2821 ccggccttcg ctgttcacgc ggtgctcgac ggcgaagtct tgtcgcacgt gcgggtcgtg
     2881 gaaccgacct aggtcatcgg ttctctcatc gtcctttcaa gaaccacgcg gtagaagggg
     2941 cgacatgaac ggtcgaatga gaatctcaat caccgccgtg ctgatcgctg ccctcgccgg
     3001 cggcgcctgg tcctccagtt cctccggttc ttccgccgag aagagcccgt cggagctggc
     3061 gccggtccac ggcagatact cgccgtccat cgacgcacgg aacttcgtgg cgaaggtcga
     3121 caaccgctac tggccgctcg agcccggtac ggcattccac tacgagggcg tgcgcggcag
     3181 aacgcgccag accgacgacg agatcgtcac acacgcgacg aagagaatcc tcggcgtgaa
     3241 gtgcaccgtg gtccgggaca ccgtctccga gcacggacgc ccggtcgagc gcacctacga
     3301 ctggtacgcc caggacaagc agggcaatgt ctggtacatg ggcgagctct cgctcgagcg
     3361 ccggcacggg cgcttcgtca aggcgagcga ctcgtgggag gcgggcgtga agggcgcgaa
     3421 gccgggcatc atcatgcccg cgaatcccaa gccgggagat cgctaccgcc aggagtacta
     3481 cccgcccggc caggcgctcg acgaggcaca cgtcctgcgg caacgtccga ccgtgacggt
     3541 gccgtacggc agcttcaagc gcgtgctcgt cacgagcgag tacagcccgc tcgagccgca
     3601 gaccgagcac aagtactacg tggccggcct cggggaggtg caggagacgg tcgtcaaagg
     3661 ccaccacgag gagttcaagc tcgtcagcgt caccaagccc tccggttgat gctctcgata
     3721 gggtgccgcg ccgatgcggc gcccactcct attcgtgacc ctgctcgcga cggtcgccgc
     3781 atgcgcggcg ggctgcggcg gaggtgaaag ccggcgaccc tccggcacca cgcacacgtc
     3841 cacggcgccg gctcaggccg ccgcgccgga gccggtcgac ggcagcgacg tgtcgttccg
     3901 ggcgtcggac gggaagaccg tgaagggagt gctcacatcg gcggggcgcc gcgctccggc
     3961 ggtgatcctg gtccacgagg tcaacggcgg cgccgatcaa tgggaccagt tcgtgccata
     4021 cctccacgaa gcgggcttca cgacgctcgc gtacgacggt cgcgggggcc tagatgaagt
     4081 cgaccttgcg cgggaggtag ccggcgcgat cagcttcctc ggcaagcgcc cggaggccga
     4141 tccgaagcgg atccaggtcg tgggcgccag catcggcgcc tctacggctg tgatgtccat
     4201 cgccacgaac gggcgtcgct ccctggccgg tgcggtggcg ctctccccgg ccgacagtcc
     4261 ctcgttcatc gagttgcagg cgaaagaccg ctacgcccca cacgacgtgc tgttcgtgag
     4321 cgacacccgg gaagcgggga atctccagaa cctggtcccg ggcgcggtgc ggtcgaagaa
     4381 gctcgagtcg gacgacccgg gtcacggggt cgcgctgctc cagcacgccg aggtgcgcga
     4441 cgcggtcctt gcgtggctca agcagcacgc ggcctgaccg cgttcccctt cacgctcacc
     4501 ccgccgagta cggagaacag gcgcaggcga atgaccg
//
LOCUS       DASVXS010000255        12549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_675253, whole genome shotgun sequence.
ACCESSION   DASVXS010000255 DASVXS010000000
VERSION     DASVXS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675253"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..444
                     /locus_tag="VF752_14630"
     CDS             148..444
                     /locus_tag="VF752_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856830.1"
                     /translation="MFTGEPTLAAGYAAGPTSLVALPPEDVRRVVATTAELGDLLLRT
                     MVTRREWLRGRGLGQQRLIGSRWSGESFAVRELLERNLVPFTWHDLATDADSRI"
     gene            585..1559
                     /locus_tag="VF752_14635"
     CDS             585..1559
                     /locus_tag="VF752_14635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8856831.1"
                     /translation="MLGGGPAGLAAAVYASSEGLSTLVVERFAPGGQAGTSARIENYL
                     GFPMGLTGAELARRATLQARKFGAMISSVHEAARVSEPHDGGFREIGLADGQRVRGRC
                     VVLAQGADYRRLPAENADRFECLGLYYAATHIEAQQCSGEDVVVVRGANSAGQAVVKL
                     ADHAREVHVVARRPLEAAMSRYLVDQIGARSNVRVWSGCEVRSLEGDEKLEAVVISGA
                     GPDRRVETSALFAMIGASPRSEGLVRFVGQDDKGFVVTGEEASRHPDFPRHWNGADRD
                     PLLLECTRPGVFAIGDVRRGSTKRVAAAVGDGALVVRSIHASLASGQA"
     gene            complement(2038..2400)
                     /locus_tag="VF752_14640"
     CDS             complement(2038..2400)
                     /locus_tag="VF752_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856832.1"
                     /translation="MRETIRERKSPGAEKEVLAKIAEMPKADRVLAERVHAIVKAAAP
                     DLAARTWYGMPAYAKDGKVVCFFQSAHKFKARYATIGFSDKAKLDKGAMWPTSFALKE
                     LAAAEEKKIRALVKKAVS"
     gene            complement(3096..3698)
                     /locus_tag="VF752_14645"
     CDS             complement(3096..3698)
                     /locus_tag="VF752_14645"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8856833.1"
                     /translation="MAEQSYDLRERKRTKTRLMIQAEAFRLFAEKGYENTTVDDIAFA
                     AAISPRTFFRYFPTKEDVVLWDEYDPIAAELVAARPDDEPLAETLRAITRAAIGGLYR
                     RDPAQLLMRTRLLASVPELRARMLAQQGSGGDMLAALLAQKRGLRADDLAARVIAAAF
                     GAAIITAVDAWQRDDGKSDLLELVDRAIHELAEGMRELGD"
     gene            3775..5214
                     /locus_tag="VF752_14650"
     CDS             3775..5214
                     /locus_tag="VF752_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX8856834.1"
                     /translation="MTTTAPTASTPVLARASDSLPPEVTKLALTLMLGGIMVALDMTM
                     VNVALDTLARDFHTSVASIQWVSTGYLLALAMVIPLTGWAVERFGAKPIWLASLAFFT
                     TGSMLCGIAWSAPSLIAFRVVQGLGGGMMMPLIQSVLARAAGPDRVGRVMGVVGVPAM
                     LGPVLGPVLGGVIVSGASWRLIFCINVPICLAALAASRRVVIPDMKRPAAFRLDMLGL
                     SLLSPGLVAIVYGLSQAGTHGSFADGHVLVPLAAGIALILAFAIHALRTRSDPLIDVR
                     LFRGRRFTSFSAVVFFFSMAMLGTALLLPLYYQQVRAQDALHAGLLLAPQGLGMGVAL
                     VVASRLTDRVSARPMILVGLVLAATATFAYTQIGAHTSVSLLSVAAFVSGAGIGAALV
                     PAMTGVYKGLRDEAVPRATSSIRIFQQLGGSFGIAILAVVLQQEAAARNTTAGLAGAF
                     GQTFWWTLAFIALALVATLMAPAEPRPRR"
     gene            complement(5305..5700)
                     /locus_tag="VF752_14655"
     CDS             complement(5305..5700)
                     /locus_tag="VF752_14655"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8856835.1"
                     /translation="MTPDNATLIVAESYAKRLTAFDIEDDGGLVNRRVWADLGDGVPD
                     GICLDAENAVWYGDVPNKRCVRVSEGGEVLQTIQLDRGCFACALGGPDGKALFMMATE
                     WNGPDEMFAGPRTGQVLMIEAPAARAGWP"
     gene            complement(5697..6155)
                     /locus_tag="VF752_14660"
     CDS             complement(5697..6155)
                     /locus_tag="VF752_14660"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8856836.1"
                     /translation="MAEPHVLMDGIVFGEQPRWHDDRLWFSDWGKPEVIAVDLHGRHE
                     MKLQAPAFPCCVDWLPDGRLLMVSARDGRLLRLEPDGSLETHGDLASASSPPAGNELV
                     VDGRGNAYVNGGGFDMMAGEPFAPGVIALVTPDGSARQSQTGSRFPTESS"
     gene            6304..6906
                     /locus_tag="VF752_14665"
     CDS             6304..6906
                     /locus_tag="VF752_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856837.1"
                     /translation="MSPEDVETLRGVRYRVSLPPERAAGRRTLDERLYVRFPVLYRLL
                     AKAMMRLPTRSRLRGKLIVRVSARAVAAGNRRDFDVLLLGFDPRIELRPLGDWAEALD
                     IPPVFYGHEGYRDVWRTMTEGFGDMWLDPEEVVDLGDYLLAMLTVRGHGSRSGVPLTL
                     PLFQLFRFRDGLVVWQRDFADRDSALEAVRRSEQDAHAYS"
     gene            complement(6897..7430)
                     /locus_tag="VF752_14670"
     CDS             complement(6897..7430)
                     /locus_tag="VF752_14670"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856838.1"
                     /translation="MSPGADLGAVFDEHVASEFESKDINATMATMVPEPYVWHVPVLT
                     GASGGEAVRDFYDTDFIGQTPDDAVLHPIARTVSENRVIDEFVLEFTHDAEVPFMLPG
                     VAPTGRKVRVPTVVVMGFEGEKIAYEHIYWDQATVLVQLGMLDPAGLPLAGGEQADRL
                     LELARSPNPKAGRKAQE"
     gene            complement(8171..8524)
                     /locus_tag="VF752_14675"
     CDS             complement(8171..8524)
                     /locus_tag="VF752_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8856839.1"
                     /translation="MRPAHLWYEPIDEHSASVTLDGDIEAPTAAALEQAVLAATRNGK
                     DCFLVDLAAVGVADASLVESLFHVGRLVENLHGKVVLVSTPGSRLRDVLRSAGLQKRM
                     RMAATRSEALTAARE"
     gene            8771..10009
                     /locus_tag="VF752_14680"
     CDS             8771..10009
                     /locus_tag="VF752_14680"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8856840.1"
                     /translation="MAVLIAATAIATVGASAGSAHKGARDSLRKIDHVVVIYEENHSF
                     DNLYGKWEGVNGLSHADPAHTRQVKQDGSTYDCLVQNNANLNTPPQSDQCQDTNPNTG
                     STFHSHFFNDSRSANDGSPFRIDSYIPASAKTCPDPSTSAPANGVQDPNGRAGGCTRD
                     LVHRYYQEQYQLNDGAQNRYTTGSDAVGLTQGYYDTRQLPIYEYLHGEDHPHYAISDD
                     FFQGAFGGSFLNHQWLIAAHTPWFDNAATSGADDLHSIADTNAMPNNYPLYKSTNAVK
                     DSSLTELCAGANPGQHDNPQRDAAHGDAVCGDWAVNTTQPRYQPFSPGTADSRRLPPL
                     HNATIGDRLTAKGVSWAWYSGGWSNAGGRPGERGWTNGSGPVTRGSNTSACPDPNSNP
                     KAVWPNCPDNLFQFHHQPFN"
     gene            10040..10561
                     /locus_tag="VF752_14685"
                     /pseudo
     CDS             10040..10561
                     /locus_tag="VF752_14685"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
     gene            complement(10624..11148)
                     /locus_tag="VF752_14690"
     CDS             complement(10624..11148)
                     /locus_tag="VF752_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856841.1"
                     /translation="MSARRSVNGAIAGAAAAAVWAAQQPLDKRLFDCRYDDVELLGKF
                     VSSDERVWPAAGLALHLQDGAVFGAVYAALRPRLPLRPVLAGMAAALAEHAALWPLVT
                     LTDRLHPARDNLPRLGESRRAWLQAAWRHVLFGAVLGLLERRLNTPEDIMEQVIPVTS
                     NGHGSIERAAAAAT"
     gene            11192..>12549
                     /locus_tag="VF752_14695"
     CDS             11192..>12549
                     /locus_tag="VF752_14695"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8856842.1"
                     /translation="MPSTFSPERAADLSRYHERTRRRGVNLFVYIPVRIVLELFVRVY
                     FRLSGTGREHIPKSGPIILAANHRSFLDPFVIGSLVRRPVYFVAKRELFEKRWQGWLL
                     NCLGAFPVCRGDSDEEMVETALSLLAAGKPIVIFPEGTRIRRGPLGHPKRGVGRFALE
                     SGAPVVPIAVTGTEKARRGWRIRPVKVKVRFGRALTFPSVENPSPRLAGEVTARIWPC
                     IQLQWEWLGGLPPLRRVAVVGAGSMGTALATLFARAGLDVDLACRTTADCERDVPDSV
                     RAVPIADLRVEEADLVTLAVPAGALPSAVAQIGDRVRDEASLLVVSKGLVPPLGTLPS
                     VYVGERVRARGVLALGGPAHAAEAVAEGASVVLAARDAHLRQQLTNVFTAAGLSVEQT
                     DDVTGTELAGTAKNVAALAAAAAAPLVAKACGDSTRITEWGQVIGWDSADFKRMKDRC
                     ETG"
BASE COUNT         1932 a         4356 c         4247 g         2014 t
ORIGIN      
        1 gcgcggggac gtcgtgttcg agcacggcca gcgcgagacc ccgctctacg tggtccagag
       61 cggagccatc gacatcatcg accacgcgcc ggagggggat cggtactcac ccagtgcagg
      121 gcgggaacgt tcgccgccga catctcgatg ttcaccggcg agccgaccct tgccgcgggg
      181 tacgcggccg ggcctacctc gctcgtggcg ctcccgccgg aggatgtgcg ccgggtcgtc
      241 gcgaccacgg ccgagctggg cgacctgctc ctgcggacga tggtcacccg ccgagagtgg
      301 cttcggggcc gcggtctggg tcagcagcgg ctgatcggct cacgatggtc gggagagtcc
      361 ttcgccgtta gggagctcct ggagcgcaac ctcgttccct tcacctggca cgacctcgcc
      421 accgacgccg acagccggat ctgatcgagg ggcttggcat cgcggaggcc gagtgcccgg
      481 tcctggtccg ctccgacagc gtcatccggc gagcgactgt agcgtcggtc gccgatgagc
      541 ttgggctacg agcgcaggtc gacggccaga gcttcgacgt cgtcgtgctc ggcggcggcc
      601 cggccgggct cgccgcggcg gtctacgcct cctccgaggg gctaagcacg ctcgtggtcg
      661 agcgcttcgc cccgggcggt caggcgggca cgagcgcgcg gatagagaac tacctcggct
      721 ttccgatggg tctcactggc gccgagctcg cccggcgggc gacgctacag gcgcgaaagt
      781 tcggcgccat gatctccagc gtccatgagg ccgcccgggt ctcagagccg cacgacggcg
      841 gctttcgcga gatcggacta gccgacggtc agcgcgtccg cggccgatgc gtcgtcctgg
      901 cgcagggcgc ggactatcgc cggctacccg cggagaacgc ggatcggttc gagtgcctgg
      961 gcctttacta cgcggcgacg cacatcgagg cgcagcagtg ctccggggag gacgtcgtgg
     1021 tcgtccgcgg agccaactcg gctgggcagg ccgtggtcaa gttggccgac cacgcgcgcg
     1081 aggtccacgt ggtggcccgg cgaccgcttg aggcggcgat gtcgcgctac ctggtcgacc
     1141 agatcggagc caggtcgaac gtccgcgtct ggtccgggtg tgaggtgcga tcgctcgagg
     1201 gcgacgagaa gctcgaagcc gtcgtgatct cgggcgcggg tcccgaccgc cgggtcgaga
     1261 cctcggcgct cttcgccatg atcggcgcct ccccgaggtc cgaggggctg gtccggttcg
     1321 tgggccagga cgacaagggc ttcgtggtca cgggcgagga ggcgagccgc caccccgact
     1381 tcccccggca ctggaacggg gcggaccgcg acccgctcct gctcgagtgc acgcgcccgg
     1441 gcgtctttgc gatcggagac gtccgcagag gctccacgaa gcgcgtggcg gcggcagtgg
     1501 gagacggcgc gctcgtcgtt cgctcaatcc acgcctcgct cgcttcaggc caagcgtagg
     1561 tcaacgatgg actgttcgtc ggcggatgaa cagccgcggc attacggcac gcagcgtcta
     1621 ctccggcggg ggctccgagc gccagctggc ggcaggctac ggtgaggatg ctgggcgctg
     1681 gaggcccgct ggccgacgag ggctggcagg agatggtctg aacggccgcc ggcggtcgac
     1741 ggcggtcgcg acccgagatc gggccagaag cggcgtttct ttggcccgat cccagccgaa
     1801 atgagtggaa agggccgaaa acgcggggct cagaccgggc cggcgcctcg cgaaaccccg
     1861 caagtaaccc aaattcagga ctgtcggacg tcggatcccg cgacgcttgc acggccgtcg
     1921 taacgccgct cgcaggcgga catcgggtga tcaccgatca tcgccgggct cgggtgataa
     1981 aacgtggcga tctccggctc gagcggacgc tcggccgcac gtccgtgagt cgagtcctta
     2041 gctcaccgcc ttcttcacga gcgcgcggat cttcttctct tcggcggcgg ccagctcctt
     2101 gagggcgaac gaggtcggcc acatggcgcc cttgtcgagc ttcgccttgt cgctgaagcc
     2161 gatcgtcgcg tacctcgcct tgaacttgtg cgcgctctgg aagaagcaga cgaccttgcc
     2221 gtccttggcg taggcgggca tcccgtacca ggtcctcgca gcgaggtcgg gcgccgcggc
     2281 cttgacgatc gcgtggactc gctcggccaa aacgcgatcc gccttcggca tctcggcgat
     2341 cttggcgagt acttccttct cggcgccggg cgactttcgc tctcggatgg tctccctcat
     2401 cgcggccttc tcctcggccg tgagtccctt tgacgctttg ccggtggctc gctcggcggg
     2461 cgtctttgtc cccttcttta cagcgttcct ctcagccatt gcgattcacc tccatcggca
     2521 tgctcattgt gtctgcatct tgccctgaat tcgcgggtca catcgaccgg atctcgttcg
     2581 tcagtgctat gacggagcac aacggaggat gtgaccgtgg accagagggt cacagcgcgg
     2641 gtctcgctcg acatgctgcg atcgcgcaag gcccggcgcg aggagtagtt tgggcggcac
     2701 ctgccggagc cgatcatcga ccccatcgac ggcacagcgc ccaaacacga ggccctcgtt
     2761 gcggattcgg tcggactcgc gctgctcgtc gtgctcgaaa cgctggatcc ggccgagcgg
     2821 ctcgcgttcg tgctgcacga catgttcggc gtcctgttcg ccgagatcgc gccgatcgtc
     2881 ggtcgctccc ggaggcggcg cggaagctcg ctagcagggc gcgtgggcgg gtgcgtagtg
     2941 ctcctgagcc agacccggac atcgcgctca acgagaggtc ggtcgatgcc ttcctcgcgg
     3001 cggcgcgcga aggagacttc gaggtcctgc tcagagtgct cgacccggat ggcgatcgga
     3061 gggtgcacca ggacgacgtg cgggcccgcg ggtcctcagt cgccgagctc gcgcatgccc
     3121 tcggcgagct cgtggattgc ccgatcgacg agctcgagca gatcgctctt cccgtcgtcg
     3181 cgctgccagg cgtcgacggc ggtgatgatc gccgctccga aggccgccgc gattacccgt
     3241 gcggcgaggt catcggctcg caaaccgcgc ttctgcgcga gcagggcggc gagcatgtcg
     3301 cctccgctcc cctgctgcgc gagcatccgc gcgcgcagct cgggaaccga ggcgagcagc
     3361 cgtgtccgca tcagcagctg cgcgggatcg cgccggtaga ggccgccgat cgccgcgcgg
     3421 gtgatcgccc gcagcgtctc ggcaagcggc tcgtcgtccg gtcgcgccgc gacgagctcc
     3481 gccgcgattg ggtcgtactc gtcccagagc acgacgtctt ccttggtcgg gaagtagcgg
     3541 aagaaggtcc gcggggagat cgcggccgcg aacgcgatgt catcgacagt cgtgttctcg
     3601 tagcccttct cggcgaagag gcgaaaggcc tcggcctgga tcatcagccg cgtcttcgtg
     3661 cgcttgcgct cgcgcaggtc gtagctctgc tccgccatcg gggtgaccgt agcacgactg
     3721 gcggcggttc ctgccaactt gacagagact gccatactgt cgtatgctga ctatatgacc
     3781 accaccgccc cgacagcctc cactcctgtc ctcgcccgag cgtccgacag cctgccgccc
     3841 gaggtcacca agctcgcgct gacgctgatg ctcggcggga tcatggtcgc gctcgacatg
     3901 acgatggtga acgtcgcgct cgacacgctc gcgcgcgact tccacacgtc ggtggcgtcg
     3961 atccaatggg tctcgaccgg ctatctgctc gcgcttgcga tggtgattcc gctcacgggc
     4021 tgggccgtcg agcgcttcgg ggcaaagccg atctggctcg cctcgctcgc gttcttcacc
     4081 accggatcga tgctctgcgg gattgcctgg tcggcaccca gcctgattgc cttccgcgtc
     4141 gtccagggcc tcggcggcgg catgatgatg ccgctgatcc agagcgttct cgcgcgcgct
     4201 gcgggcccag accgcgtcgg tcgcgtcatg ggcgtcgtcg gcgtgccggc gatgctcggg
     4261 ccggtgctcg gtccagttct cggcggcgtc atcgtcagcg gcgcaagctg gcgcctgatc
     4321 ttctgcatca acgtgccgat ctgcctggcc gcgctcgcgg cctcgcggcg tgtggtgatc
     4381 cccgacatga agcggcccgc tgccttccgg cttgacatgc tcgggctctc gttgctgtcg
     4441 cctggacttg tcgcgatcgt ctatggcctc tcccaggcgg gcacgcacgg cagcttcgcc
     4501 gatggccacg tgctcgtccc gctcgccgcc ggcatcgcgt tgatcctcgc gttcgcgatc
     4561 cacgccctgc gcacccgcag cgacccgctg atcgacgtcc ggctgtttcg cggacggcgc
     4621 ttcacgagct tctcggcggt tgtcttcttc ttcagcatgg cgatgctcgg gacggcgctc
     4681 ctcctcccgc tctactacca gcaggtccgc gcccaggacg cgctacacgc cggcctgctg
     4741 ctcgcgccgc agggtctcgg gatgggcgtc gcgctcgtcg tcgcgagccg gctgacggac
     4801 cgcgtcagcg cgcggccgat gatcctcgta ggcctcgtgc tcgccgctac ggcgacgttc
     4861 gcctacacgc agatcggcgc gcacacgagc gtctcgctgc taagcgtcgc ggcgtttgtc
     4921 agtggcgccg gcatcggcgc ggcgctcgtc cccgcgatga ccggcgtcta caagggccta
     4981 cgtgatgaag ccgttccgcg cgcgacgagc tcaatccgga tcttccagca gctcggcggc
     5041 tcgttcggca tcgcgatcct cgccgtcgtc ctccagcaag aggccgccgc tcgcaacacc
     5101 acggccggac tagccggcgc gttcggtcag actttctggt ggacgctggc cttcatagcg
     5161 ctcgcgctcg tggcgacgct gatggcccct gcggaacctc ggccacgtcg ctgagcgcgg
     5221 cgccagggtt cccaagggac atcgtgagga gactgcagcg agcatccacc acttcttgtt
     5281 ctccggcgtt atgaggcgct gcatctaggg ccagccggcg cgcgctgccg gcgcttcgat
     5341 catgagcacc tgacctgtcc gcggaccggc gaacatctcg tcggggccgt tccactcggt
     5401 cgccatcatg aagagcgcct tcccatccgg gcctcccagc gcgcaggcga agcagccccg
     5461 gtcgagctgg atcgtctgga gcacctcgcc gccctcagag acccgaacgc aacgcttgtt
     5521 gggcacgtcc ccgtaccaga cggcattctc ggcgtcgaga cagatcccgt cgggaacgcc
     5581 gtctccaagg tcggcccaga cgcgccggtt cacgagccca ccgtcgtctt cgatgtcgaa
     5641 ggccgtcagt cgcttggcgt acgactcggc gactatcagc gtcgcgttgt cgggcgtcac
     5701 gaggattccg ttgggaaacg cgagcccgtc tgcgactggc gggcagaacc gtcgggggtg
     5761 accagcgcga tgacgccggg cgcgaacggc tcgccggcca tcatgtcgaa gccgccgccg
     5821 tttacatatg cgttgccgcg accatcgacg accagctcgt tgccggccgg aggggaggag
     5881 gcgctcgcga ggtcgccgtg ggtctcgagc gagccgtcgg gctcgagccg taagagacgc
     5941 ccatcgcggg ccgaaaccat gaggaggcgg ccgtccggga gccagtcgac gcagcaggga
     6001 aacgccggcg cctgcagctt catctcatgc cttccgtgca gatcgactgc gatgacctcc
     6061 ggtttccccc agtcggagaa ccagagccga tcgtcgtgcc agcgtggctg ttcaccgaac
     6121 acgatgccgt ccatcagaac gtgcggctcc gccatcgcgg tcaccgtgca ccgtcgctgg
     6181 tctggaccgc cgctgggtgg agtggggcta gccagccgct ctgtccccgg tcgtggcgac
     6241 ctcggcgtcc ttcttcacgc gggctcacgc cgactcctga cgctgcggga tactgggcgg
     6301 gcgatgtcgc cggaggacgt ggagacgctc cgtggcgtgc ggtaccgcgt atcgctgccg
     6361 ccagaacgag cggctgggcg acggacgctg gacgagcgcc tgtacgtgcg ctttcccgtt
     6421 ctgtacaggc ttctcgcgaa ggcgatgatg aggttgccga cgcggtctcg actgcggggc
     6481 aagctgatcg tccgcgtctc ggcgcgggca gtagctgctg gcaatcgccg ggacttcgac
     6541 gtgctcttgc tcggcttcga tccgaggata gaactgcgtc ctctcggcga ttgggccgaa
     6601 gcgctcgaca tacctccggt tttttacggt cacgagggtt accgagacgt atggcgcacg
     6661 atgacggaag gattcggaga catgtggctc gaccccgaag aggtggtaga cctcggtgac
     6721 tacttgctcg cgatgcttac cgtgagagga cacggttccc gcagcggcgt gcccttgacg
     6781 ctgccgctgt tccagctctt caggttccgc gacggtctgg tggtctggca gcgtgacttc
     6841 gcggaccgcg acagcgccct cgaagcagtg cgccgctcgg agcaagacgc tcacgcctac
     6901 tcttgagcct ttcttcccgc cttcggattc ggcgagcgcg cgagttccag cagccggtcg
     6961 gcctgctcgc cccctgccag agggagcccg gctggatcca gcatgcccag ctgaacgagc
     7021 accgtcgctt ggtcccagta gatgtgctcg tacgcgatct tctcgccctc gaagcccatg
     7081 acgacaacgg tggggacccg caccttgcga cccgtcggag cgaccccggg cagcatgaac
     7141 gggacctccg cgtcgtgggt gaactcaaga acgaactcgt cgatcactcg gttctcggag
     7201 accgtgcgcg cgattgggtg cagcacggcg tcatccggcg tctggccgat gaagtccgtg
     7261 tcgtagaagt cccgcacggc ctcccctccc gacgcgccgg tcagcaccgg cacgtgccag
     7321 acgtacggtt ccgggaccat cgtggccatc gtcgcgttga tgtccttgct ctcgaactcg
     7381 ctcgccacgt gctcgtcgaa gacggctccg aggtcggctc cggggctcat ctcatacctc
     7441 cagtttcgcg tcccggtgtg acatcgccgg cgacgctacc gctcggcgcc gccggcggcg
     7501 accgcgctgc ttcgaggcag gccccgagca gctatcgggg ggtagacgta accgacccag
     7561 acggcctatc gtcggcgatg ccggggcggt gacactcacc cggacggcaa cgccccgccg
     7621 gacgggggtt cgtggcacca gaccgacaac ccacgccctg accttgtcgc tttacggagg
     7681 cgagtgatcg acgaggcacc gcgtgggagc tggcacccat ggccgccccg gcctttcatc
     7741 cgcaacgagc ggactaaacg cgtatggctt cgtcaccccg tgcttgtcca ctgggtacaa
     7801 cgaactcgcc agaggtagtc cgcaagcccc gtggagacct ctccgcgagc gcgggcccac
     7861 catacgccat ggattgctcg ccgcttccac ggcgacgcgc ggcagcggtg ggcccgtcgc
     7921 cttcggcccg aggtcgaaga ctacgccccc ggcgcggaag cacggcacgg cttgcggctg
     7981 gtttcgggcc gaaagaccgt agtcaagccg actccgactg agcggaactg agaggaacta
     8041 gtttcgctct acagcgcggg gaatccccct agccctgtgc ttacaaaacc attgctctac
     8101 cagctgagct actccggcac agagaatcag agcctagcgg cgcggatggc ctccgggacc
     8161 gtctacgagc tcactctcta gccgcagtca gcgcctcgct gcgagtcgct gccatacgca
     8221 tccgcttctg gagcccggcg ctgcgaagaa cgtctcgtag gcgggacccg ggcgtcgata
     8281 ccaagaccac tttcccgtgc aggttctcca ccagacgacc gacatggaag agggattcga
     8341 ccagactggc gtccgcaacg ccgaccgcgg ccagatccac gaggaagcag tccttcccgt
     8401 tccgggtcgc ggccaagaca gcttgctcga gcgcagcggc ggtcggggcc tcgatgtcgc
     8461 cgtcgagcgt gaccgacgca ctgtgttcgt cgatgggctc gtaccagagg tgggccgggc
     8521 gcatttccct tgggataccg cggagcgcga agctggaaac tcgcaatttg cggaattcgc
     8581 actcggcgct gctcgagccg acttctcgag gcgggcctag ccgaacgttt ctccaaccct
     8641 tcacggcgga gtaactggcg cgtaacggga cgggccgcgc ggttcgaaat cgtcctgccg
     8701 cctcgcaagc gacgtcagga gagacagagt gccatcaccc aagttgccag ggcggcgagg
     8761 tgcggccacg atggctgtgc tgattgccgc caccgctatc gcgacagtgg gcgcgagcgc
     8821 cggctccgcc cacaagggcg cgcgagacag cctccgaaag attgaccacg tggtggtcat
     8881 ctacgaggag aaccacagct tcgacaacct ctatgggaaa tgggagggag tgaacgggct
     8941 ctcgcacgcg gaccccgccc acacccgcca ggtgaagcag gacggcagca cctacgactg
     9001 cctagttcag aacaacgcca acctgaacac gccgccgcag agcgatcagt gccaggacac
     9061 gaacccgaac accggcagca cgttccacag ccacttcttc aacgacagcc ggtcggcgaa
     9121 cgacgggagc ccgttccgga tcgacagcta catcccggcg agcgcgaaga cgtgcccgga
     9181 cccgagcacg tcggctccgg cgaacggtgt ccaggatccg aacggccgcg cgggcggctg
     9241 cacgcgcgac ctcgtgcacc gctactacca ggagcagtac cagctgaacg acggcgcgca
     9301 gaaccgctac acgacgggca gcgacgcggt cggacttacg cagggctact acgacacccg
     9361 gcagctcccg atctacgagt acctgcatgg cgaggaccat ccgcactacg cgatctccga
     9421 tgacttcttc cagggcgcgt tcggcgggtc gttcctcaac caccagtggc tgatcgcggc
     9481 gcacacgccg tggttcgaca acgcggcgac ctcgggcgcg gacgacctcc attcgatcgc
     9541 ggacacgaac gcgatgccga acaactatcc gctctacaag tcgaccaatg cggtcaagga
     9601 ctcgtcgctc acggagcttt gcgccggcgc gaatcccggc cagcacgaca acccgcagcg
     9661 ggatgccgct cacggggacg ccgtttgcgg cgactgggcc gtgaacacga cccagccccg
     9721 gtaccagccg ttctccccgg gaaccgcgga ctcgagacgg ctgccgccgc tccacaacgc
     9781 gacaattggc gaccgcctca ccgcgaaggg cgtgtcatgg gcttggtact ccggcggctg
     9841 gtcgaacgcc ggcggccgcc cgggcgagcg gggctggacg aacgggagcg gccccgtcac
     9901 gcgcggaagc aacacgagcg cgtgcccgga cccgaactcc aacccgaagg cggtgtggcc
     9961 gaactgcccg gacaacctgt tccagttcca ccaccagccg ttcaactagt acgccaactg
    10021 tgcgccgggc acggaagccc gcgagcagca cctgcgcgac gagcaggagt tcatctccaa
    10081 ggcgaacgcg tcgcggcaga gctgcaacct tgaccaggtc tcgttcatca agccgatcgg
    10141 tgccgagaac gagcaccccg gctacgcaag cgagccgaac ggcagcgatc acctcgtgaa
    10201 cctgctgaag gcaatcgagg gcggcgcgtg tgcgaaggac acgatggtcg tcgtcaccta
    10261 cgacgagttc ggcggagcat gggaccacgt gccgccgccc ggccagggtg gcacgatcgg
    10321 cccgcacgac gcgtgggggc cgggcacccg gatcccagcg ctcacgctgg ctccgcacct
    10381 gcgcggcgac ttcgtcgtgg accaccgcca gcacgacacc acctcgatcc tcgccacgat
    10441 cgagcaccgc ttcgggctcg agccgctcgg ctcgcgcgac gcagcggtga acgacctctc
    10501 gagcgtgttc tacgcgcacg aggccacgac ggcgagcgcc gcgaggcggt accaccgctg
    10561 accaaggcca ggtgagtcag ccgcgacggc cccgctccgg cggggccgtc gtcgtcttgc
    10621 acgtcaggtg gcggccgccg cggcgcgctc gatgctccca tggccgttcg aggtcacggg
    10681 tatgacctgc tccatgatgt cctcaggcgt attcaggcgc cgctcgagca agccgagcac
    10741 ggcgccgaag agcacgtgac gccaggcggc ctgcagccac gcccgccggc tctcgccaag
    10801 acgcggcaga ttgtcgcgtg ccggatggag ccggtcggtg agcgtcacga gcggccaaag
    10861 cgccgcgtgc tccgcgagcg cggcggccat cccggccaac accggtcgca ggggcagccg
    10921 cggtcgcaga gccgcgtaga ccgcgccgaa cacggcgccg tcctggaggt gcagcgcgag
    10981 cccggctgca ggccacacgc gctcgtcgct cgagacgaat ttcccgagca gctccacgtc
    11041 gtcgtaccgg cagtcgaaga gccgcttgtc gagcggctgc tgagcggccc acacagctgc
    11101 tgccgcggcg cccgcgatcg caccgttcac cgacctccgc gcgctcatcc accgcagcgt
    11161 aacgagagcc ccgcaaaggg gtaagtcagg catgccgtca acgttctccc ctgagcgcgc
    11221 ggccgatctg tcgcgctatc acgagcgcac gcgtcgccgt ggcgtgaacc tgttcgtgta
    11281 catcccggtg cggatcgtgc tcgagctgtt cgtgcgcgtg tacttccgcc tgagcggcac
    11341 agggagggag cacatcccga agagcggccc gatcatcctc gcggcgaacc accgaagctt
    11401 cctcgatccg ttcgtgatcg gctcgctcgt ccggcggccg gtctacttcg tggcgaagcg
    11461 cgagctcttc gagaagcgct ggcagggctg gctgctcaac tgtctcggcg cgttccctgt
    11521 atgccggggc gactcggacg aggagatggt ggagacggcc ctctcgctgc tcgcggccgg
    11581 caagccgatc gtgatcttcc cggagggcac gcgcatccgt cgtggcccgc tcggccatcc
    11641 gaagcgcggt gtcggacgct tcgcgctgga gagcggagcg ccggtcgtgc cgatcgccgt
    11701 gaccgggacc gagaaggcgc gccgcggctg gcgcatccgc ccggtcaagg tgaaggttcg
    11761 cttcggccgg gcgctcacgt tcccgagcgt cgagaacccg tcgccgcgac tcgccggcga
    11821 ggtgaccgca cgcatctggc cgtgcatcca gcttcagtgg gagtggctcg gcgggctgcc
    11881 gccgctccgg cgcgtggcgg ttgtcggcgc cggctcgatg ggcaccgccc tggccacgct
    11941 cttcgcgcgc gcgggactcg atgtcgacct cgcctgccgc acgacggcgg actgcgagcg
    12001 cgacgtgccg gactcggtgc gggccgtgcc gatcgccgac ttgcgcgtgg aggaggccga
    12061 cctggtgacg ctcgcggtcc cggccggcgc cctcccgagc gccgtcgccc agatcggcga
    12121 ccgggttcgc gacgaggcat cgctgctcgt cgtctcgaag gggcttgtac cgccgctcgg
    12181 cacgctgccg tccgtgtacg tgggcgagcg cgtgcgtgcg cgcggtgtac tcgcactcgg
    12241 cggcccggct cacgccgccg aggcggtcgc ggagggcgcg tcggtggtgc tggccgcacg
    12301 cgacgcgcac ctccgtcagc agctcacgaa cgtcttcacc gcagcggggc tctctgtcga
    12361 gcagaccgac gacgtgaccg gcactgagct cgccggcacg gcgaagaacg ttgccgcgct
    12421 cgccgccgcg gccgccgcgc cgctggtggc caaggcatgc ggcgacagca cgcgcatcac
    12481 cgagtggggc caggtcatcg gctgggacag cgcggacttc aagcgcatga aagaccgctg
    12541 cgagaccgg
//
LOCUS       DASVXS010000256         6938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1099277, whole genome shotgun sequence.
ACCESSION   DASVXS010000256 DASVXS010000000
VERSION     DASVXS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099277"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VF752_14700"
     CDS             complement(<1..700)
                     /locus_tag="VF752_14700"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX8856843.1"
                     /translation="MSDPPPQEQRAVQRVALVLRPERDLKDAVEEVCAWAARIGVALF
                     GPDSEVRLPPEVERRSADSLAAGCDAVLSLGGDGTMLGALRLAATHGVPVLGVNLGTL
                     GYLTEVDGAHLGDALSALGEGSYTIESRTGLSLAARSGMDAQIACNDVVISRVPGRGQ
                     AALGLHIDGELLVRYASDGVIAATPQGSTAYAFAAGGPLVSPRAQGMIVTPDAPHGLF
                     NRAVVLAEGECLGIE"
     gene            835..1167
                     /locus_tag="VF752_14705"
     CDS             835..1167
                     /locus_tag="VF752_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856844.1"
                     /translation="MPVSTAEKVQALSHDFGSQRRLAELLDVSPAQVTRWIRGQGIDE
                     QNAERVDLLELVMSNLLRLYRADAAASWLVGLNPNLGDRRPIDLIRTGRARELLDAIA
                     NERAGGFA"
     gene            1290..1721
                     /locus_tag="VF752_14710"
     CDS             1290..1721
                     /locus_tag="VF752_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856845.1"
                     /translation="MYASTERLAVVVERLAPFRGTGELTDAMLGQAELPLAISELELA
                     DDVMAIDLDDPAVLVSERLRPSGVATGHRPVTQRMAADLFDRHGDAGALLWWSALEAS
                     WLNATVFDRAHDALLVRATEELTVEHPLVREAATFLGLRTP"
     gene            1757..2551
                     /locus_tag="VF752_14715"
     CDS             1757..2551
                     /locus_tag="VF752_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX8856846.1"
                     /translation="MTVRTPAREQSPPSFLRLAADPLRWRLLSELARSDRRVRELCGL
                     LEQRQSLISYHLGQLRAEGLVASRRSAADGRDSYYVLDLARCRELLASSGTTLHPALR
                     LADSPPREWSRASRSSQPRVLFLCTGNSARSQIAEALVQELTRGAVDASSAGSHPKPL
                     HPNAVRVMRERGVDLGGRRSKHLDEFADKRFDYVISLCDRVREVCPEFHGHPDLIHWS
                     IPDPAREGSTDEETYPAFQRTASELATRIPFLLEVIAHTTSTQEVT"
     gene            2554..2937
                     /locus_tag="VF752_14720"
     CDS             2554..2937
                     /locus_tag="VF752_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8856847.1"
                     /translation="MSEDKLVSVRYMVDDVQEAIDFYTRHFGFTLRHSALPAFADVVR
                     GKLRLLLAGPESSAGRPMPDGRKPGPGGWNRIHLIVDDIASEVERLRSAGLSFRNDIV
                     TGPGGRQILLDDPSGNPIELFQPAG"
     gene            2976..3332
                     /locus_tag="VF752_14725"
     CDS             2976..3332
                     /locus_tag="VF752_14725"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX8856848.1"
                     /translation="MNYSVNEIDPTAPPSGTGCVECLAGSGPGWWLHLRRCAACGHVG
                     CCDNSPSQHGTAHSRETGHPVLQSFEPGEDWFYDYRSGEFFDGPELAAPHSHPADQPT
                     PGPAGAVPPDWQLHLN"
     gene            3419..4438
                     /locus_tag="VF752_14730"
     CDS             3419..4438
                     /locus_tag="VF752_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8856849.1"
                     /translation="MRRLIVCALVVGAAVIAPTGAGADDPSSLQYCTPAGGPLGAITD
                     AEHTVVLGDQPLPRGVTRTRETVDGVSTPVMQAGPADAHGAVVFVHGNPGSSRDFDQL
                     VGATGKFTRVVAFDMPGFGHADKGFDGTYTTQSTVQFIGDMVAHLGITRVHLALHDFG
                     GIWALEWARANPGSLGSVVLMDTGVLTGYLGHPFAYIWNVPAIGEENMATTTRQTFES
                     TLNAQNPRPLPQAFLDRMYDDYDRQTRCAVLSYYRDLGNSGESRWEAQTAVFSKLDLP
                     ALVIWGADDPYIPSSQAENQKNAFPQAEIHVLPDTGHWPFVDEPGTTRNLVTSFLRRV
                     VARRP"
     gene            4473..4739
                     /locus_tag="VF752_14735"
     CDS             4473..4739
                     /locus_tag="VF752_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856850.1"
                     /translation="MAESNVDLARNGFDAALGGDLDALGEVLDPDVKWHGGDPSAPGS
                     CQNRDQVLGFMRQLEVVQAGGVELVDLVGAGDRRDLPPLLAGGR"
     gene            complement(4722..5477)
                     /locus_tag="VF752_14740"
     CDS             complement(4722..5477)
                     /locus_tag="VF752_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8856851.1"
                     /translation="MKIVVIGGTGLIGSKLVAKLADQGHEAIPASPDTGVNTLTGEGL
                     SEVLEGAQVVVDVSNAPNWDDDAVMHFFETSSRNLLAAETAAGVVHHVALSVVGTDRL
                     QESGYFRAKLAQEKLIEGSSIPYSIVHATQFFEFIKGIADAATDGNTVRLPPALIQPM
                     ASDDVASGVGQVAVGPPLNGVVEIAGPEQFRLDELIRGRLKATNDPREVTTDPTATYF
                     GITPGERTLLPGADARIAETRLDDWLNQSAAAS"
     gene            complement(5526..6812)
                     /locus_tag="VF752_14745"
     CDS             complement(5526..6812)
                     /locus_tag="VF752_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HEX8856852.1"
                     /translation="MAEPRIVVANREDLWWLLAEAVQLEQMIMCQYLFAEFGLKAGTD
                     EGLTEEQAQAVDRWRKVLHGIAVEEMLHMALVANVMSAIGAAPSFGSPNFPQTSGYFP
                     SGIQLALIPFGEQALEHFLYLERPEGMERQDAASFVPGAPPREPLRADELMPRGQEYA
                     TIGHLYRGIEDGLRSLSSRLGERAAFVGSPRAQATPELFHWPQLVSVLDLDSALAAVT
                     EIIEQGEGARGDWQQAHYGRFLAMWEEYRELRKRDASFEPARPVLAAYTRQPFDVPEP
                     QPVITDAVTHQVAELAAVAYELVIHLLTRFFTHTDETEEQLGVLVRGAIGLMGGVLRP
                     LATAMTTLPVGEGHPGQTAGFAFEMYYRMSNFVPSREPAWALLNERLSLLADGCAEAC
                     SNPGIPAAVEAAGERATAIRETIVAHVPAELQARSG"
BASE COUNT         1098 a         2367 c         2356 g         1117 t
ORIGIN      
        1 cctcgatccc gagacactcg ccctcggcga gaaccactgc ccgattgaag agcccatgcg
       61 gagcgtcggg cgtgacgatc atgccctgtg cccgtggaga gacaagcggc ccgcccgccg
      121 cgaacgcgta agcggtcgag ccctgcggcg tggctgcgat gacgccgtcg cttgcgtagc
      181 gcacgaggag ttcgccgtcg atgtgcaggc cgagtgccgc ttggccgcga cccggcacgc
      241 ggctgatcac gacgtcgttg caggcgatct gcgcatccat gccgctccgc gcggcgagcg
      301 acaacccggt gcgcgactcg atcgtgtaac tgccctcgcc gagtgccgag agcgcgtcgc
      361 caaggtgcgc gccgtcgacc tccgtcaggt aaccgagcgt tccgagattg acgcccagca
      421 ctggcacgcc gtgggtggcg gcgaggcgca gcgcccccag catcgtgccg tcaccgccga
      481 gcgacagcac ggcgtcacag cccgcggcga gcgagtcagc ggaccgccgc tcgacttcgg
      541 gcggcaggcg aacttcgctg tccgggccga acagcgcgac tccaatgcgt gccgcccacg
      601 cgcagacctc ttcgactgcg tctttcaagt cgcgctcggg gcgaagcacg agcgctacac
      661 gctggacggc tcgctgctct tgcggcggtg gatcggacat cctgcccagc agtcaagcat
      721 tcgcgccgct gcgagtggtc gtccctcgcg ttctggacga tgcggacatc gcgcgcttcg
      781 ctcggcgcaa cactacttgt tgcgcaacga atttcgttgc gctagcctgg gcgcatgccc
      841 gtgagtaccg cggagaaggt ccaggcgctg agccatgact tcgggtcgca gcggcggctt
      901 gcggagctac tggacgtgag ccccgcgcag gtcacgcgct ggatccgtgg gcaagggatc
      961 gacgagcaga acgccgagcg ggtggatctc ctcgagctcg tgatgtccaa cctgctgcgg
     1021 ctgtatcgcg cggacgcggc ggcaagctgg ctggtgggtc tgaacccgaa cctgggggac
     1081 cgccggccga tcgatttgat ccgcacggga cgcgcacgcg agctgctcga cgccatcgcc
     1141 aacgaacgcg ccggaggctt cgcctgatcc tctggcggtc gctgccgttc gatcgcaacg
     1201 cggcacacga cgagcctggg ggagcgctct cgtttccgcg tcgtttccag ggcgcgggac
     1261 ggcacgacaa ccccgaccta tacggctgca tgtacgcgtc cacagagcgg ctcgcggtcg
     1321 tcgtcgaacg gctggctcct ttccgaggga caggcgagct caccgacgcg atgctgggtc
     1381 aggcggagct cccgctcgcg atcagcgaac tcgaactcgc cgatgatgtg atggcgatcg
     1441 acctcgatga tcctgccgtg ctcgtgagcg agcggcttcg cccgtcaggg gtagcgaccg
     1501 gacaccggcc cgtcacgcaa cggatggccg cggatctgtt cgaccggcac ggcgacgctg
     1561 gcgcgctgct gtggtggtcc gccctcgagg cgagctggct gaacgcgacc gtcttcgacc
     1621 gcgcgcacga cgcgttgttg gtgcgagcga cggaggagtt gaccgtcgag catcctttgg
     1681 tgcgtgaagc cgcgacgttt ctgggactcc gaacgccgtg aaatgcatca agccagattg
     1741 atgtattctc cgcgcaatga ccgtccggac gccggcgcgc gagcagtctc cgccgagctt
     1801 tctgcggctc gcagctgatc cgctccgctg gcggctgctg agcgagcttg ctcggagcga
     1861 tcgccgcgtc cgcgagctgt gcgggctgct cgagcagcgg cagagcctga tctcctacca
     1921 cctcggacag ctgcgagccg aggggctggt cgcgagccgc cgcagcgctg ccgatggccg
     1981 cgactcctac tacgtactcg acctcgcgcg ctgccgcgag ctgctcgcga gtagcgggac
     2041 gacgctgcat ccagcgctgc gcttggccga ctcgcccccg cgcgagtgga gtcgcgcatc
     2101 gcgctcgagt cagcctcgtg tgctgttcct gtgcaccggc aacagcgccc gctcgcaaat
     2161 cgccgaggcg ctcgttcagg agctcacgcg cggcgcggtc gatgcctcga gcgccggtag
     2221 ccatcccaag ccactccacc ccaacgccgt gcgggtgatg cgcgagcgcg gggtggacct
     2281 tggcggtcgg cgctccaagc acttggacga gttcgccgac aagcgcttcg actatgtgat
     2341 cagcctctgt gaccgcgtgc gcgaggtctg ccctgagttc cacggccacc ccgacctcat
     2401 tcactggagc atccccgacc ccgcgcggga gggctcaacg gacgaggaga cctaccccgc
     2461 gtttcagcgc accgcgagcg aactcgccac tcgcatcccg ttcctgctcg aggtgatcgc
     2521 acacaccacg tcaactcagg aggtgacctg aagatgtccg aagacaagtt ggtcagcgtt
     2581 cgttacatgg tcgacgatgt gcaggaggcc atcgacttct acacccgcca cttcggcttc
     2641 acgctgcgtc acagcgcgct gccggcgttc gccgacgtcg tgcgcgggaa gctgcggctg
     2701 ctgctcgccg ggccggagag ctcggccggg cgaccgatgc ccgacggccg caaaccgggc
     2761 ccagggggct ggaaccgcat ccacctgatc gtcgatgaca tcgccagcga ggtcgagcgc
     2821 ttgcgctcgg ccggactctc atttcgcaac gacatcgtga ccggcccagg cggccggcag
     2881 atcctgctcg acgatccctc cggcaacccg atcgagctct ttcagccagc cgggtaaccg
     2941 atgggcgtgg cgcgccgtac tctccgaaag tgacgatgaa ctactccgtc aacgagatcg
     3001 atccgaccgc tccgccgagc ggcaccggct gtgtggagtg tctcgccggc tcggggccgg
     3061 gctggtggct gcatctccgc cgctgcgctg cgtgcggtca cgtgggctgc tgcgacaact
     3121 cgccgtcgca gcacgggact gcgcacagca gggaaaccgg tcaccccgtg ctgcagagct
     3181 tcgaaccggg cgaggactgg ttctacgact accgcagcgg cgagttcttc gacggacccg
     3241 agctggcggc gccgcacagc catcccgcgg atcagccgac cccgggaccc gccggcgcgg
     3301 tgccgccgga ctggcagctc cacctcaact gactgaggtc ggttcgagcg ccaagcctgc
     3361 tgggtataag caacggaatg accggcgcgg gggccggtcg cgcttcaggg ggcaggggat
     3421 gcgaagactg atcgtttgcg cgttggtcgt cggggcagcg gtgatcgcgc ccaccggcgc
     3481 gggcgcggac gacccgtcgt cgttgcagta ctgcacgccg gccggaggac cgttgggcgc
     3541 gatcacagac gcggagcaca ccgtcgtgct cggcgaccag cctctgccgc ggggtgtcac
     3601 ccgcacgcgc gagacggttg acggcgtctc aacgccggtg atgcaggccg gaccggcaga
     3661 cgcgcacggg gcagtcgtct tcgtccacgg caacccgggc tcgtcgcgag acttcgacca
     3721 gctcgtgggg gcgacgggga agttcacgcg cgtcgtcgcg ttcgacatgc ccggcttcgg
     3781 tcacgccgac aaaggattcg acggcaccta caccacgcag agcacggtgc aattcatcgg
     3841 cgacatggtc gcgcacctcg gcatcacgcg cgtgcatctc gccctccacg acttcggcgg
     3901 catctgggcc ctcgagtggg ccagggccaa ccctgggagc ctcggcagcg tggtgctgat
     3961 ggacaccggc gtgctcaccg gctacctggg ccatccgttc gcatacatat ggaacgtgcc
     4021 ggcgatcggg gaggagaaca tggcgacgac gacgcgccag acgttcgagt cgaccctgaa
     4081 cgcccagaac ccgcggccac tcccgcaggc gttcctggac cggatgtacg acgactacga
     4141 ccggcagacc cgttgcgccg tcctcagcta ttaccgcgac ctgggcaatt ccggcgagtc
     4201 gcgttgggag gcacagaccg cggtgttcag caagctcgac ctgccggcac ttgtcatctg
     4261 gggtgcggac gacccgtaca taccgtcctc gcaggcagag aaccagaaga acgcgttccc
     4321 ccaggccgag attcacgtgc tgccggatac gggacactgg ccgttcgtgg acgagccggg
     4381 cacgacgcgc aatctcgtga cgtcgttcct acggcgagtg gttgcccgtc ggccgtaatc
     4441 tgcgcggcgc cggcggtggg acaattcccg ccatggctga gtccaacgtg gacctcgccc
     4501 ggaacggttt cgacgcggcg ctgggaggcg atctcgacgc cctcggcgag gtgctcgacc
     4561 cggacgtgaa gtggcacggc ggcgatccga gcgcacccgg ctcctgccag aaccgcgacc
     4621 aggtgctcgg gttcatgcgc caattggagg tcgtgcaggc cggcggcgtc gagctcgtcg
     4681 atctcgtcgg cgctggcgac cgccgggatc tcccgccgct cctagctggc ggccgctgac
     4741 tggttcaacc agtcgtcgag acgcgtctcg gcgatgcgcg cgtcagcgcc ggggaggagg
     4801 gtgcgctcgc cgggcgtgat gccgaagtag gtcgcggtcg ggtcggtggt gacctcgcgc
     4861 gggtcgttag tcgctttcag tcttccgcgg atgagttcgt cgaggcggaa ctgctcgggt
     4921 cccgcgatct ccacgacgcc gttcagcggt gggccgacgg cgacctggcc gactccgctc
     4981 gcgacgtcgt cggaagccat gggctggatg agcgccggcg ggagccgaac ggtgttgccg
     5041 tcggtggccg cgtcggcgat gccctttatg aactcaaaga actgcgtcgc gtggacgatc
     5101 gagtagggga tcgaggagcc ctcgatcagt ttctcttggg cgagcttcgc gcggaagtag
     5161 ccgctctcct ggaggcggtc ggtaccgacg accgacagcg ccacgtgatg caccactccg
     5221 gcggccgtct ctgctgcgag aaggttgcga gacgacgttt cgaagaagtg catcaccgcg
     5281 tcgtcgtccc agttgggagc gttggagacg tcgacgacga cttgtgcgcc ctcgagcact
     5341 tcggacagcc cttcgccggt gagcgtgttg acgccggtgt cgggtgacgc tgggatcgcc
     5401 tcgtgcccct ggtcagcaag cttggcgacg agctttgatc cgatcaggcc ggttccgccg
     5461 ataaccacga tcttcatgtc attcctttcg cttgtgatcg agatgcgctg gcggggccga
     5521 gacgcctatc ccgatcgggc ctgaagctcg gcgggcacgt gagccacgat cgtctcgcgg
     5581 atcgcggtcg cgcgctcgcc ggccgcctca acggcggcag ggattcccgg gttgctgcag
     5641 gcctccgcgc acccgtccgc gagaagcgac agacgttcgt tcagtagcgc ccatgcgggt
     5701 tccctcgagg gcacgaagtt gctcatccgg tagtacatct cgaacgcgaa cccggctgtc
     5761 tgacccgggt gtccttctcc caccggcagc gtcgtcatcg cggtcgcgag cggacgtagt
     5821 acgccgccca tcaggcctat cgcgcccctg accagcacgc ctagctgctc ctccgtctcg
     5881 tcggtgtgcg tgaagaaccg ggtcagcaga tggatgacaa gctcgtacgc aacagccgca
     5941 agctcggcca cctgatgcgt cacggcgtcg gtgatgacgg gctgaggctc cgggacgtcg
     6001 aacggctgac gcgtgtaggc agccagcacc gggcgcgccg gctcaaacga cgcatcacgc
     6061 ttgcgcagct cgcggtactc ctcccacatc gcgaggaatc tgccgtaatg cgcctgttgc
     6121 cagtcgcccc gggcaccctc gccctgttcg atgatctccg ttaccgcggc gagcgcggag
     6181 tcgaggtcga gcaccgagac cagctgcggc cagtgaaaca gctccggcgt tgcctgcgcc
     6241 ctgggcgacc ccacgaacgc agcgcgttcg ccaagcctgg aggagaggct gcgcagaccg
     6301 tcctcgatgc cgcggtagag gtgtcctatc gtcgcgtact cctggccgcg gggcatcagc
     6361 tcatcggccc gcaacggctc gcgcggtggc gcgcccggga caaagctggc cgcgtcttgg
     6421 cgctccatgc cctcgggccg ttcgaggtac aagaagtgct cgagtgcctg ctcgccgaac
     6481 ggaatcagcg ccagctggat tccggaagga aagtagccgg aagtctgggg gaagttcggg
     6541 gacccgaagc tcggcgcggc gccgatggcg gacatcacgt tggcgaccag cgccatgtgg
     6601 agcatctcct ccaccgcgat cccgtgcagc accttgcgcc agcggtccac cgcctgggcc
     6661 tgctcctcgg tcaggccctc atcggtgcca gccttaaggc cgaactccgc gaacaggtac
     6721 tggcacatga tcatctgctc gagctgcacc gcctcggcga gcagccacca caggtcctcg
     6781 cggttagcta cgacgatccg gggctccgcc atgcgatgcc tcagtacgac gcgagcagcc
     6841 ggttggcctc agcgagggcg tcggtcaagc cctgcacacc gagcacgacc gcagcgccgt
     6901 cctggccggg ctcgatccgg agcacgaggt cgccgtct
//
LOCUS       DASVXS010000257         6728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1034353, whole genome shotgun sequence.
ACCESSION   DASVXS010000257 DASVXS010000000
VERSION     DASVXS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034353"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VF752_14750"
     CDS             <1..396
                     /locus_tag="VF752_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8856853.1"
                     /translation="DADSRIARIRWRQATAIGIAQAAALVPGISRSGITMGGGLIVGL
                     SNDDAARYAFLLATPIIGAAAALKLPELLGSEGNGVRGPAAVGALCAGVTTYFAVKFL
                     LRFFETNRLTPFAVYCVTAGVICSLIFAL"
     gene            complement(393..638)
                     /locus_tag="VF752_14755"
     CDS             complement(393..638)
                     /locus_tag="VF752_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856854.1"
                     /translation="MTKNRALIWPAVAAGIVLLAIAVIYWVDPAKSLPSFFPGHTAGS
                     NHHHVKHGIAAFVVAMACFVFAWFQSGPGPTNRPAAS"
     gene            complement(724..2400)
                     /locus_tag="VF752_14760"
     CDS             complement(724..2400)
                     /locus_tag="VF752_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX8856855.1"
                     /translation="MHVDTDAEVLRLRGLLRDLVALSAIPAAWIGSDPPAVAAGLADA
                     LVGMLQLDFAFVCLSGSGGAGAVEVTRGSAWKRFPEWLEGHLATSAPLPPKEIVPDVG
                     DDSVLRRGFVVPIGVNGEGGVVAAASERSDFPTQIDQLLLSLAANHAAAAFQSARIID
                     ERKKAEEQLRNARNELEEKVAKRTAELQVANDELSALRRVATLVAEGAAPSAVFDAVA
                     AEMERLLEADQVVLSRYEPGAEVTVVAHRGASARRVPPGTRVSHDGENVQAMVRRTEQ
                     PARIDNFELAHGTIGELARTVGVRAAAGAPIVVEGRLWGVISASWSREESPPADTEER
                     MARFAELLDAAIVNADSRDQLSASRARLVTEGDEARRRVVRDLHDGAQQRLVHTILTL
                     KLAQRALRDKAGNAESLVGEALQHAEQGNAELRELAHGILPAVLARGGLRAGVDAFVE
                     RLDLPVKVELPARRFPAEIEASAYFIVAEALTNIVKHSQATRAEVRASVCNGMLNVEV
                     RDDGMGGADPEGHGLVGLRDRATALGGRLKIESTLGGGTVVVATLPVSVG"
     gene            complement(2401..3057)
                     /locus_tag="VF752_14765"
     CDS             complement(2401..3057)
                     /locus_tag="VF752_14765"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX8856856.1"
                     /translation="MKADDNSLRIEPNGLDGANRHICAFFNGMDEHYRVLRSFIKHGF
                     EEGDKAFHLVDPKRREDHLRRLTDAGIDVEEATASGQLEVHPWEDGPLHGDRFDQDTW
                     LAAFEQALQSGPASGYAKTRFLAQMEWALVDLPGIDDMIEFETRVNYVVPKYDDPVIC
                     SYDLSKFGASSVIYALRTHPIVIIGGLLQENPFYVDPDELLAEIREQRPASAGKSIAR
                     "
     gene            3583..4146
                     /locus_tag="VF752_14770"
     CDS             3583..4146
                     /locus_tag="VF752_14770"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX8856857.1"
                     /translation="MSKGRLEAFSDGVFAIAITLLVLEIPVPPTEEGKSLAHALLDRW
                     PSYAGFVISFVTIGIIWVNHHAVIARVQSVDRTLLFKNLLLLLTVSFIPFPTAVMAEF
                     LRSANGQDVAVAFYSASFCVMGIAFRQLWTPAARNWVSRIGPTMYLIAAGVAFLNAYV
                     ALGICAGLALYYSLPISIEQQLSQSSS"
     gene            complement(4127..5683)
                     /locus_tag="VF752_14775"
                     /pseudo
     CDS             complement(4127..5683)
                     /locus_tag="VF752_14775"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
     gene            5743..6297
                     /locus_tag="VF752_14780"
     CDS             5743..6297
                     /locus_tag="VF752_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX8856858.1"
                     /translation="MDTDSLRGQLLIAGPTLLDPNFARTVVLVTEHNDEGAMGLVLNR
                     PSTSTVDEALPDLELDVAEPEDLVYVGGPVQPAAAMILAEFDEPGEAATIVLEDVGFV
                     SVEPELGGGSVATRRARVFAGYAGWGPGQLESEVERDDWILEPATVEDVLGDSAEELW
                     SDALWRKGGEYALLSRMPLDPSLN"
     gene            6308..>6728
                     /locus_tag="VF752_14785"
     CDS             6308..>6728
                     /locus_tag="VF752_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8856859.1"
                     /translation="MDRPGQVAVGTWSGGRFMHFGEPLDDARFEALVRPAGGIDTVMT
                     ADVYGQGEADSLLGRALRGVPRGDYFLIGAIGHDFYEGERDGAKGFPRFTDPSLRGPD
                     GYADYVRMATERSLERCGADSFDLLLLHNPDRIGYTSE"
BASE COUNT          996 a         2384 c         2206 g         1142 t
ORIGIN      
        1 gacgctgact cgcgcatcgc ccgcatccgc tggcgccagg cgaccgcgat cggaatcgct
       61 caggctgcgg ctctcgtgcc gggcatctcg cgctccggca tcacgatggg cggcgggctc
      121 atagtgggcc tctcgaacga cgacgccgcc cgctacgcgt tcctgctcgc cacgccgatc
      181 atcggcgccg ctgcagcact gaagctgcca gagctgctcg gctcggaggg caacggcgtg
      241 cgcggcccgg ctgcggtcgg ggccctatgc gctggcgtga cgacctactt cgcggtgaag
      301 ttcctgctgc gcttcttcga gacgaatcgt ctcacgccgt ttgccgtcta ctgcgtgacg
      361 gccggcgtga tctgctcgct gatcttcgcg ctttagctcg ccgcgggccg gttcgtgggg
      421 ccggggccgc tctggaacca ggcgaagacg aagcaggcca tcgcgactac gaacgcggcg
      481 attccgtgct tcacgtggtg gtggttcgag ccggccgtgt gcccggggaa gaacgacggc
      541 agcgatttgg cggggtcgac ccagtagatc accgcgatcg ccaggaggac gatgccggca
      601 gcgaccgccg gccagatcag cgcacggttc tttgtcatag gcgcgaacgt atgaagggaa
      661 cataaagcgg gtctaagggc tgcgttgctt gctgtcccca gctgaggcag gctcgctgtg
      721 gctctagccg actgagaccg gcaacgtggc gaccacgacc gtgccgccgc cgagcgtact
      781 ctcgatcttc agccggcccc caagggcggt cgcccggtcg cggagcccca cgagcccgtg
      841 accctcggga tcggcacctc ccatcccgtc gtcgcggacc tcgacattga gcatcccgtt
      901 gcagacagat gccctgactt cggcgcgggt ggcctgtgag tgcttgacga tgttcgtcag
      961 tgcctccgcc acgatgaagt aggcgctcgc ctcgatctcc gccgggaacc gccgggccgg
     1021 gagctccact ttgaccggta ggtcgagccg ctcgacgaat gcgtcgaccc cagcccgcag
     1081 cccgccgcgc gcgaggaccg cgggaaggat cccgtgcgcc agctcgcgga gctccgcgtt
     1141 cccctgctcc gcatgttgca gcgcctcgcc gacgagcgac tcggcgttgc ccgctttgtc
     1201 tcggagcgct cgctgggcca gcttcagcgt cagaatggtg tgcaccagcc gctgctgcgc
     1261 gccgtcgtgc aggtcccgca ccacgcggcg gcgtgcctcg tccccctcag tcacgagacg
     1321 cgcccgcgac gcgctgagct ggtcgcggct gtcggcgttc acgatggcgg cgtcgagtag
     1381 ctcggcgaac ctggccatcc gctcttcggt gtcggccggc ggtgactcct cgcggctcca
     1441 gctcgccgag atcacgcccc agagccggcc ctctacgacg atcggcgctc ccgcggccgc
     1501 gcgcacaccc accgtgcgcg ccagttcacc aatggttccg tgcgctagct cgaaattgtc
     1561 gatccgggca ggctgctccg tgcgccgcac catcgcctgc acgttctcgc cgtcgtggct
     1621 cacacgcgtc ccgggcggca ctcgccgtgc gctggccccg cgatgggcga ccacggtgac
     1681 ctcggcgccg ggctcgtagc ggctcagcac gacctggtca gcttccagca accgctccat
     1741 ctccgctgcc acggcgtcga agaccgcgct gggcgcggcg ccttccgcca ccagagtcgc
     1801 aacgcggcgc agcgcgctca actcgtcgtt ggccacctgc agctcggcgg tccgcttggc
     1861 caccttctct tcgagctcgt tgcgtgcgtt acggagctgt tcttctgcct tttttcgctc
     1921 gtcgatgatg cgagcgctct ggaacgccgc cgcggcgtgg ttcgcagcga gcgatagcaa
     1981 cagttggtct atctgcgtgg gaaagtcgct gcgctcggag gcggcggcga ccacacctcc
     2041 ctcgccgttg acgccgatcg ggacgacgaa gccgcgtcgc agcaccgagt cgtcgccgac
     2101 gtcgggaacg atctccttgg ggggcaacgg agcgctcgtg gcgaggtgac cttccagcca
     2161 ctccggaaag cgcttccacg cgctccctcg cgtgacctcg actgctccag ccccaccgga
     2221 gccgctcagg cacacgaacg caaagtcaag ctgcaacatg ccgacaagtg cgtcggcgag
     2281 cccggccgcc accgcgggcg gatcgcttcc gatccacgcg gccgggatcg cggacagcgc
     2341 gacgaggtcc ctcaggaggc cccggaggcg gagaacttcg gcgtcggtat cgacgtgcac
     2401 ctagcgcgcg atggatttgc ctgcgctcgc cggccgctgc tcccgtatct cggcgagaag
     2461 ctcgtccgga tccacgtaga acgggttctc ctgcagcaat ccaccgatga tcacgatcgg
     2521 atgcgtccgc agggcgtata tcaccgagct cgcgccgaac ttcgacaggt cataggagca
     2581 gatcaccggg tcgtcgtact tggggacgac gtagttgacc cgcgtctcga actcgatcat
     2641 gtcgtcgatg cccggcaggt cgacgagcgc ccattccatc tgcgccagga acctcgtctt
     2701 ggcgtacccg gatgcgggcc cggactggag cgcttgctcg aaagcggcga gccacgtgtc
     2761 ctggtcgaac cggtcaccgt ggagcggccc gtcctcccac gggtgcacct cgagctggcc
     2821 tgaggccgtg gcctcttcca cgtcgatgcc tgcatccgtt aggcgcctga gatggtcctc
     2881 gcgtcgcttg ggatcgacga gatgaaacgc cttgtccccc tcctcgaagc cgtgcttgat
     2941 gaacgaacga aggacgcggt agtgctcgtc catgccgttg aagaacgcgc agatgtggcg
     3001 attcgcgccg tcgagaccgt tcggctcgat ccgcaaagag ttgtcgtcgg ctttcatacc
     3061 ccacctccac ccggcgagcg ccgggttgct tccagaacgt caggccctac ggcgaccctc
     3121 ccattcgagc gcgtagggag caccagtgcg cgacgatgaa gggccccttc tctcctagcg
     3181 gttcggtgca gcgctacggg gcaatatctt tcgacggccg agcttcggag cgtccgcctc
     3241 tgcatgctgc tcgattctga gggggcggaa tcgcccacgc tactctccgg tgcgctccgg
     3301 gtcaacgacc gcgatgaggc gcgccacgta ccacatggcg cgaagctagg gcctggcgga
     3361 tccgactcaa ggcgagtacg cgatcgcgcg ataacgccgg acaatcacgg tttagggcca
     3421 ttgatcgcgc ggggtcagcc tcagcgcact ccactcggcg tccgtgagcc gcggcgctcc
     3481 aacaccccgc caccagcgcg tgcggtcagc gggatccgta tcggagcgtc gcggctcgcc
     3541 gtacgcggaa ggtgaacccg cggccaagcc gaacgtgcac gcatgagcaa gggccggctc
     3601 gaggcgttca gcgacggcgt attcgcgatc gcgatcaccc tgctcgtgct cgagatccca
     3661 gtgcccccga cggaagaggg caagtcgctc gcgcacgcgc tgctcgaccg ctggccgtct
     3721 tacgccggct tcgtgatcag cttcgtcacg atcgggatca tctgggtgaa tcaccacgcg
     3781 gtgatcgcga gagtccagag cgtggaccgc actttgctct tcaagaacct gctgctgctc
     3841 ctcaccgtct cgttcatccc gttcccgacc gcggtgatgg ccgagtttct acgctcggcc
     3901 aacggccagg acgtggccgt ggcgttctac tccgcctcgt tctgcgtgat gggcatcgcg
     3961 ttccgccagc tctggacacc cgcggcccga aactgggtgt cgcggatcgg gccgacgatg
     4021 tatctcatcg ccgccggcgt ggcgttcctc aacgcgtacg tggcactcgg gatctgcgcc
     4081 gggctcgcgc tctattactc gctaccgatc tcgatcgagc agcagctcag tcagagctct
     4141 tcctagcccc gcgcccgaac tgcagcacca gcagtcgctc gagcagcttg gtgcgcgact
     4201 tcttgtaggg caggaagttc gggtcccgct tcatgccgaa gcgcgtgacg aggatcgagt
     4261 gcgttcggca gtacttctgg atgcccttcg cgccgtggcg cgcacccagg cccgactcct
     4321 tcaccccgcc gaacggctgc tcgagggcca ggtagttcac gagcgcgtcg ttcacgcagg
     4381 tcgccccggc ctgcacctgg cgagccacct gctcgcccct gcgcacgtcc ttcgaccaca
     4441 cgctcgagtc gaggccgtag ggcgagtcgt tcgccagcct cacggcctcg tcggcgtcgc
     4501 gcaccttcat gatcggcagc gtcgggccga aggtctcctc ggtcatgatc agcatcgagt
     4561 gatccacgtc ggcgagcacg gtcggctgga agaactgacc cggcccgtcg ccacggctgc
     4621 cacccgcgag gatgcgtgcg cccttctcgc gcgcgtcggt cacgtgcttc tcgatcacgt
     4681 cgacctgctc gtcgaaggta atcgcgccga cgtcgaccga gcccgcctcg ccgggcacac
     4741 cctgacgcag gtcgcgcgcc ttctcgccca ccttctcgac gaactcgtcg tagatcttct
     4801 cgtgcgcgta gacgcgctcg accgagatac aggtctggcc gctgttggag agcccgtagg
     4861 tgatcgcggc gttggcggcg cgctcgaggt cggcgtcgtc gagcacgatc atcgggtcct
     4921 tgccgccgag ctcgagcgcg gccgggatca ggcgcttggc cgcctgctcg gcgatcttgc
     4981 ggcccgtctc cgtggagccg gtgaacatga tcatgtcgca cgtgtccacg agcgctccgc
     5041 cggcaccgcc cgagccgcac gcgatcgaga acacgtcctc cgggcagccg gacgcgcgca
     5101 tgccctccgc catcagcagc gagctgtagg gcgtcacgct cgagggcttc aacaccacgg
     5161 cgttgccggc catcagcgcc ggaatcgcgt cgccgaagtt gttcacgagc gggtagttcc
     5221 aggggccgat caccccgacc acgcccatcg gccggtagcg cacgacgagc ttcttgccga
     5281 gcagcatcgg cgagtgcggc ctgatgcgct cgtcgcccag gtacttctcc gccttcttcg
     5341 cccagaagcc gagcgagtcg gacgtgtaga gcagctcctg cagcagcgcg tcctcgtagg
     5401 tcttgccgct ctcctcgatc agggtgctaa tcacgcgctc gcggttctcc atcacccagg
     5461 cgcgcatgtt cagcatcacg cgcgcgcgac ccttgaagcc gagcgcgtcc cacgcaggct
     5521 gcgccgcgcg tgccttcgac acgagctccg cgacctgctc ggcgcccatg ttcgggagcg
     5581 tcccgatcac cgctccggtg gccgggttga tgacggtgat ctcgccggcg ctgcccgcgc
     5641 cgttggctga ggcctcggtg gcccgttcga ccgtggacat ccggacttgc tccttcgtcg
     5701 cctgaagaga cgcgacatct tattcagggc acaatggggg tcgtggacac ggactccctc
     5761 cgaggacagc tcctcatcgc cggacccacg ctgctcgacc cgaacttcgc ccgcaccgtg
     5821 gtgctcgtga ccgagcacaa cgacgaaggc gcgatggggc tggtgctcaa caggccgtcc
     5881 acctccaccg tggacgaggc gctgccggac ctcgagctgg acgtcgccga gcccgaggac
     5941 ctggtgtacg tgggcggtcc ggtccagccc gcggcggcaa tgatcctggc ggagttcgac
     6001 gagccgggcg aggccgcgac gatcgtgctc gaggacgtgg ggttcgtgtc cgtcgagccc
     6061 gagctcggcg gcgggtcggt cgccacccgc cgggcgcgcg tgttcgccgg ctacgccggc
     6121 tgggggcccg gtcagctcga gagcgaggtc gagcgcgacg actggatcct cgagccggcg
     6181 accgtggagg acgtgctcgg cgactcggcc gaggagctct ggagcgacgc gctgtggcgc
     6241 aagggcggcg agtacgcgct gctctcgcgc atgccgctgg acccttcgct caactagatt
     6301 cacgttcgtg gatcgaccag gtcaagtagc cgtcggcacc tggagcggcg gtcgcttcat
     6361 gcatttcggc gagccgctcg acgacgcccg cttcgaggcg ctcgtgcgcc cggcgggcgg
     6421 catcgacacg gtcatgacgg ccgacgtcta cggccagggc gaggccgact cgctgctcgg
     6481 ccgcgcgctg cgcggcgtgc cgcgcggcga ctacttcctg atcggcgcga tcggccacga
     6541 cttctacgag ggcgagcgcg acggagccaa gggcttcccc cgcttcaccg atccctcgct
     6601 gcgcgggcct gacggctacg cggactacgt tcgcatggcc accgagcgca gcctcgagcg
     6661 ctgcggcgcc gactccttcg acctgctgct acttcacaac cccgatcgca tcggctacac
     6721 gagcgagg
//
LOCUS       DASVXS010000258         5787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_823727, whole genome shotgun sequence.
ACCESSION   DASVXS010000258 DASVXS010000000
VERSION     DASVXS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823727"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VF752_14790"
     CDS             complement(<1..403)
                     /locus_tag="VF752_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856860.1"
                     /translation="MDIDEPSLGAARALQRALPGPSFVERSGGGNLHVWVFFSEPVEA
                     YVASGVLAQAARETGVTVDVCPALDEWRATSHIKLPYHGDSRPMLDNAGRPVPLVTFL
                     EACEGRRTNAAAWRASAQRLGLAPPRPARSGR"
     gene            complement(914..1483)
                     /locus_tag="VF752_14795"
     CDS             complement(914..1483)
                     /locus_tag="VF752_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8856861.1"
                     /translation="MKPNVDITDPRIAKALAHPLRLQILEILQERDASPSEIAEEIDA
                     PLGNVSYHVRQLASFGLVKLVRETPRRGAVEHHYRAEARPRITDGAWEAMPDIVKQAT
                     IASTLDHIGRQVRGAAENGAFDAPDTHLSRSRLLVDDRGRRELAELGVDLLERVQKIE
                     EESTKRLEAANHEGERPSELVLMVFDSPS"
     gene            complement(1624..2550)
                     /locus_tag="VF752_14800"
     CDS             complement(1624..2550)
                     /locus_tag="VF752_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8856862.1"
                     /translation="MSLTVVGSIAFDAVKTPFGERERMLGGSAVHFSLAASFFTDVRV
                     VGPVGDDFGEPEYDALRQRSVNVDDVERVDGGKTFFWAGEYGWDLNTRETLATELGVF
                     GEFSPKLSEEARAADTLFLANIQPDLQRQVRRLCEGARLVGLDSMNLWIETARDSLVA
                     AIGEVDLVFMNDSELRQLTEQPNLVRAAREVMAMGPRVIVAKQGEYGAALFSSEGFFA
                     LPAYPLETVVDPTGAGDSFAGGFLGYLASQGEGAAEDDSALRRAMTYGSVIASFNVEE
                     FGTERVQRLTRDEIEERFAEFKAMTHFESVPA"
     gene            complement(2589..4040)
                     /locus_tag="VF752_14805"
     CDS             complement(2589..4040)
                     /locus_tag="VF752_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8856863.1"
                     /translation="MDEPIDHRGDALFALPQLPATAAVFGDPPGWRSDLSVRGIELVD
                     GRAPELAVAGPEHVGEAISSGARAVIVDASRTAATALRRAGLRVRRLLPMPIHGTPVL
                     YLNLDQRVAAGYGIVSRGTTTGRLRIIRDRVAAVAARSGALARAVPAVAVAAPSDGAP
                     ALLRAAREIGLPEGAAWNMVVSPGSAIRRNAFLLFDPGSSEPDYALKFSRVPDLTVQF
                     DREERGVAVARAAGTTVASVMPTHMGRVEVRGHHASLETAARGVRLTMFLRGPHEFEA
                     KVRALEQMVTWLEDVARDTASPPATLAPERDRIAREVMPDYADRVPGDFVDKVPPIPS
                     VFCHNDPSEENIVLSPGGLTLLDWEWAQQHGLPLADLVYFGTGVLRILDRPEREEDRV
                     PHFVELMQGRASSSQRMFRWVARMSDAVGLPATAVGQVVTLGILEHGNASRRERHRFE
                     QATGSSFAPALAERVADAWLREPGLGPAWDAWR"
     gene            4069..5244
                     /locus_tag="VF752_14810"
     CDS             4069..5244
                     /locus_tag="VF752_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856864.1"
                     /translation="MSVRSTSRRSGRAAELLDGLLEQPALVIGSPPPHGHDLDLLASS
                     DQERTLRTMLAGAGFTRQDVAPFRGYEEGFRIWARFSGCDADAVDEIPIGNLELPPEE
                     EERLFAEARSIPGFARLVRPAPHHVLLILAHNLLDPGDGSRPLASGRRARVRRALEEE
                     PDAWERAGELAPAWSSMQRLARLRAGFDLAEAAAAGQLQSRPAAAVPGLRARLRAARA
                     YRRAWLYGRVIGFSGPDGAARSAQAEGLRRALDALDIPARLERTPSPRARRRIPLPAH
                     ASAAVVAVTHAGVLRRAVLPGLRAGEVVICDRYTLDASVLVRSHFGERRRFRLQTALM
                     HLLTPRPRRAYLIDSPGPKAVLYREHGELLGVRTLDGTRARDDICADVALDVWQAVR"
     gene            complement(5257..5655)
                     /locus_tag="VF752_14815"
     CDS             complement(5257..5655)
                     /locus_tag="VF752_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8856865.1"
                     /translation="MPRDLLEQARTGYELFARGAYDEAFEVFDPEIEWVEWQALPGAT
                     VRHGHDEVRAYLEEIRESFAEIHYDLEDAELDEPYVIAQIHVHGIGRMSGLPVSARVV
                     HVWRTGSDGRAVQLRVFGSMEEAREALASR"
BASE COUNT          837 a         2091 c         2003 g          856 t
ORIGIN      
        1 agcgcccgct ccgggcgggc cgtggaggcg cgagtccgag gcgctgggcg gacgcgcgcc
       61 aggcggcggc gttggtgcgc cggccctcgc acgcctcgag aaacgttacg agcggcacag
      121 gccgtccggc gttgtcgagc atcgggcggg agtcgccgtg gtagggcagc ttgatgtggc
      181 tcgtggcgcg ccactcgtcg agcgccgggc acacatcgac cgtcacaccg gtctcgcggg
      241 cggcctgggc gagcacgccg gatgccacgt acgcctccac cggctctgag aagaacaccc
      301 agacgtgcag gttgccgcct ccagagcgct cgacgaagct cggaccggga agcgcgcgct
      361 gcagcgcacg cgccgcaccc agcgatggct cgtcgatgtc cactgctgcc aaccagcatc
      421 tgtcgtcggc gtgcaacgga gcaatcgcca gccccgggcc tgtgcccgcc aggtgccgct
      481 cgacggcttc gggtgtcggc ggcccctcga ccggttccct cgctcccgtc cagtgagcgt
      541 caggcgaccc cgcgaatacc cgcaggaagc gctctattag ggcacttcgg tcgtcgtgct
      601 ggggagcgag tgccggcacg gggcttcgtg cttcccgacg cgcacacgca ttggcgaggc
      661 gggcgttggg tctccgtgcg ggccacgtcg gcgcggcgtg ccgggcagtg gcgtgcgcgg
      721 gtgcggcgga taacccgtac cgccatcgtc gtctcccggg tcgatcggct cgcccttccc
      781 tgcccaccag cacagccaga tggccgccac gacggggatc ttgaggatca gaaacagaaa
      841 tacgaacgtc cagaccaccg cagctggatc gtacccgtgg tcccgctggc tgaaaagggg
      901 gattccgcgc gctttaggag ggcgagtcga acaccatgag aacgagctcc gacgggcgct
      961 cgccttcgtg gttggcggct tccagccgct tcgtgctctc ctcctcgatc ttctggacgc
     1021 gttcgagcag gtccacgccg agctcggcca gctcgcgccg tccccggtcg tcgacgagca
     1081 ggcgcgagcg ggagaggtgc gtgtccggcg cgtcgaaggc gccattctcg gcggcgccgc
     1141 gcacctgccg cccgatgtga tcgagcgtgg acgcgatcgt ggcctgcttg acgatgtccg
     1201 gcatcgcctc ccaggcgcca tcggtgatcc gcggccgcgc ctccgcgcgg tagtggtgct
     1261 caaccgctcc gcgccgcggc gtctcgcgca ccagcttcac gaggccgaag gaggcgagct
     1321 ggcgcacgtg gtagctaacg ttgccgagcg gtgcgtcgat ctcctcggcg atctcgctgg
     1381 ggctcgcatc gcgctcctgg aggatctcga gaatctgcag ccggagagga tgcgcgaggg
     1441 ccttcgcgat tcgcggatcc gtgatgtcga cgtttggctt catggcgcgg ggaggggagc
     1501 gcgctctggc gcgcccgcgc tcataaatcg aagagtactg gggccgctct aactgattgt
     1561 taaggcgcgc cgtttgtgca gagctttcgc accttcgggc agcgcctttt gctcgcgaac
     1621 gctctacgcc ggcacggact cgaagtgcgt catcgccttg aactcggcga agcgctcctc
     1681 gatctcgtcg cgggtcaggc gctgcacgcg ttccgtgccg aactcctcca cgttgaacga
     1741 ggcgatcacc gacccgtagg tcatcgcccg ccggagcgcg ctgtcgtcct cggccgcgcc
     1801 ttcgccctgc gacgccaggt agccgaggaa cccgcccgcg aagctgtcac cggcgccggt
     1861 cgggtcgacc acggtctcga gcgggtaggc cggcagcgcg aagaagcctt ccgagctgaa
     1921 gagcgccgcc ccgtactcgc cctgcttggc gacaatcacg cgcggcccca tcgccataac
     1981 ttcgcgcgcg gcccgcacga ggtttggctg ctcggtgagc tgccgcagct ccgagtcgtt
     2041 catgaacacg agatccacct cgccgatcgc ggccacgagc gagtcgcgcg cggtctcgat
     2101 ccagaggttc atcgagtcga gacccacgag ccgggcgccc tcgcacaggc ggcgcacctg
     2161 gcgctgcagg tcgggctgga tgttcgccag gaagagcgtg tccgccgcgc gcgcctcctc
     2221 cgacaacttc ggcgagaact cgccgaacac gcccagctcg gtggcgagcg tctcacgcgt
     2281 gttcaggtcc cagccgtact cgcccgccca gaagaaggtt ttgccgccgt ccacgcgctc
     2341 cacgtcgtcg acgttcacgc tgcgctggcg cagcgcgtcg tactccggct cgccgaagtc
     2401 gtccccgacc ggccccacca cccgcacgtc ggtgaagaac gacgccgcca gcgagaagtg
     2461 cacagccgag ccgccgagca tccgctcgcg ctccccaaag ggggtcttaa cggcatcgaa
     2521 ggcgatggat ccgacgacgg tgaggctcat ggcggtgcgg aaccctagcg gctcgcgatc
     2581 gcgaccaatc agcgccacgc gtcccaggcc ggaccgaggc ccggctccct gagccacgcg
     2641 tccgccacac gctccgcgag cgccggagcg aaactactcc cagttgcttg ctcgaagcga
     2701 tgacgctcgc gacggctcgc gttgccgtgc tcgaggatgc ccagggtgac cacctgtccg
     2761 accgccgtcg ccggcagccc caccgcgtcc gacatccgag cgacccagcg gaacatccgt
     2821 tgcgaactag aagcccgtcc ctgcatcagc tcgacgaaat gcgggacccg gtcctcctcg
     2881 cgctcggggc ggtcgaggat ccggagcacg ccggtgccga agtagacgag gtcggccagg
     2941 ggcagcccgt gctgctgcgc ccattcccag tcgagcaacg tcagcccgcc ggggctgagg
     3001 acgatgttct cctcgctcgg gtcgttgtgg cagaacaccg aggggatcgg tggaaccttg
     3061 tccacgaagt cgcctgggac gcggtcggcg taatcgggca taacctcgcg cgcgatccgg
     3121 tccctctcgg gagccagggt ggcgggcggg gaagccgtat cgcgggcaac gtcttcgagc
     3181 caggtaacca tttgctccag cgcgcgcact ttcgcttcga actcatgcgg gccgcgcagg
     3241 aacatcgtga gccgtacacc gcgagcggcc gtctcgagcg acgcgtggtg gccccgcacc
     3301 tccacgcgcc ccatgtgggt gggcatgacg gaggcgacgg tcgtccccgc ggcacgcgcg
     3361 accgcaacgc cacgctcctc gcggtcgaat tgcaccgtca gatccggcac ccgtgagaac
     3421 ttgagcgcat agtccggctc gctcgagccg gggtcgaaca gcaggaacgc gttccgccgt
     3481 atcgcgctcc cgggggagac gaccatgttc cacgcggccc cttcgggcag gccgatctcg
     3541 cgtgccgctc gcaggagcgc cggcgcaccg tcgctcggcg ctgcgactgc gacggcgggt
     3601 actgcgcggg cgagcgcgcc cgagcgcgcc gcgacggcgg ccacacggtc gcgaatgatg
     3661 cgcaatcgcc ccgtggtcgt cccccggctg acgatcccat aaccagcggc gacgcgctgg
     3721 tcgaggttca ggtacagcac aggcgtcccg tggatgggca tcgggagcag cctgcgcacg
     3781 cgcagccctg cccgccggag cgcggtcgcg gccgtccgcg acgcgtcgac gatcactgct
     3841 cgggccccgg acgagatcgc ttcgccgaca tgctccgggc cggccacggc cagctcgggg
     3901 gctcgcccgt cgacgagctc gattccgcgg accgaaagat cgcttcgcca ccccggcggg
     3961 tcaccgaaca ccgccgcggt cgccgggagt tgcggcaacg cgaagagcgc gtcgccacgg
     4021 tgatcaatcg gctcgtccat ggcctccggc gatcatagtt ttgcggagtt gagcgtgcga
     4081 agcaccagcc ggaggtccgg aagggcggcg gagctgctcg acggtctgct ggagcagccg
     4141 gcgctcgtga tcggatcgcc gccgccgcac ggccacgacc tcgacctgct cgccagttcg
     4201 gaccaggaac gcacgctgcg gacgatgctc gcgggcgcgg ggttcacccg ccaggacgtg
     4261 gcgccgttcc ggggttacga ggagggcttc cgcatctggg cgcgtttctc cgggtgcgac
     4321 gccgacgcgg tggacgagat cccgatcggc aaccttgaac tgccccctga ggaggaggaa
     4381 cggctcttcg ccgaggcgcg gtcgatcccg gggttcgctc gcctggtgcg gcccgccccg
     4441 catcatgtgc tgctcatcct cgcccacaac ctgctcgacc cgggcgatgg cagccgaccg
     4501 ctggcgagcg gtcgccgcgc gcgagtccgg cgcgcgctcg aggaggagcc ggatgcatgg
     4561 gagcgggccg gagagctagc tccagcgtgg agctcgatgc agcggctcgc ccgcttgcgg
     4621 gccggtttcg acctggcgga agccgctgcc gccggccagc tgcaatcgag gcccgcggcc
     4681 gccgttcccg ggctgcgcgc acggctccgc gccgcgaggg cctaccggcg cgcctggctc
     4741 tacggccgcg tgatcggctt ctcaggcccg gacggcgcgg cgcggagcgc ccaggccgag
     4801 ggcctacgca gggccctcga cgcgctcgac atccccgcga ggctcgagcg aactccttcc
     4861 cctcgcgcgc gccgccgcat ccccctgccc gcgcacgcct cggcggctgt ggtcgcagtc
     4921 acccacgccg gggtgcttcg ccgcgccgtg ctcccgggcc tgcgcgcggg tgaggtcgtg
     4981 atctgcgacc gctacacgct cgacgcgtcg gtgctcgtcc gctcgcactt cggcgagcgc
     5041 cggcggttcc ggctgcaaac ggcgctgatg cacctgctca ccccccgacc gcgccgcgcg
     5101 tacctgatcg attcgccggg gccgaaggcc gtgctctatc gcgagcatgg cgagctgctc
     5161 ggcgtccgga cgctcgacgg gacgcgcgcg cgggatgaca tctgcgcgga cgtcgcactc
     5221 gatgtctggc aagcggtgcg ctgatcgtcc ggcgccctag cggctcgcga gcgcctcgcg
     5281 cgcctcctcc atcgagccga acacgcgcag ctggacagcc cgcccgtcgg acccagtccg
     5341 ccacacgtgc acgacccgcg cgctcaccgg tagcccgctc atccggccga tgccgtgcac
     5401 gtggatctgt gcgatcacgt agggctcgtc gagctccgcg tcctcgaggt cgtagtggat
     5461 ctccgcgaaa gactcacgga tctcctccag gtaggcacgc acctcgtcgt gcccatggcg
     5521 cacggtggcg ccgggaagcg cctgccactc gacccactcg atttccgggt cgaacacttc
     5581 gaacgcctcg tcgtaggcgc cgcgcgcgaa cagctcgtag ccggtgcgcg cctgctccag
     5641 gaggtcgcgg ggcacgcggc cagtatgtac acgtaagctc cctgccgcca tgcgcgcgat
     5701 ccagatcgag gagttcggtg ggccggaggt gatgcggctc gtggagctcc cggtgcccga
     5761 gccgtccgac ggcgagctgc tggtcaa
//
LOCUS       DASVXS010000259        14668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_680464, whole genome shotgun sequence.
ACCESSION   DASVXS010000259 DASVXS010000000
VERSION     DASVXS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14668
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680464"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..372
                     /locus_tag="VF752_14820"
     CDS             67..372
                     /locus_tag="VF752_14820"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX8856866.1"
                     /translation="MSAAWATVIVVGVATIVIKGTGTFILHGRSLPASLEGPIAMLAP
                     VLLAALVATQAFGGDRELVVDDRALGLGAAAVLVALRAPTLVVVIAAAAVTALARAI"
     gene            386..697
                     /locus_tag="VF752_14825"
     CDS             386..697
                     /locus_tag="VF752_14825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX8856867.1"
                     /translation="MTRFGLIGQFLAQPGKGDALAAILLEAARGLESNPDCELYAIHR
                     SPDEPDSVWVTEVWKSREAHGASLEDPGAKELIQQARPIIAGIGERRELQPLGGKGIG
                     D"
     gene            complement(694..2013)
                     /locus_tag="VF752_14830"
     CDS             complement(694..2013)
                     /locus_tag="VF752_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8856868.1"
                     /translation="MSLPPGPRMPAAVQTLNWIVRPEAFMRESAALYGEAFTVRLAQI
                     GRLVFVWDPDELKRIFTADRGVLRAGEANFTLEPVLGEHSVLLLDGAEHLRQRKLMLP
                     PFHGERLVRYEELMRGITDRVIDSWPIGRPFRLQPSMQDITLDVILRVIFGLEESART
                     EDVRRLLKRMMHVSTRPWALVPVLRDDRWPVGPYATFRRARDKVDAAIYRLIAERRRD
                     PDLEERDDILSMLMLARDEDGQPMTDVELRDELMTLLLAGHETTATALAWAFERLLHT
                     AGSFERVRDERYADAVVKETLRLRPPIPIVGRHVVEPFEVDGYEIPRGEFLAPCVYLT
                     HRRPDLYPEPDEFRPERFLDRSPDTYGWIPFGGGIRRCLGASFAMFEMKVVLGAVASR
                     AVLEPERAEAEPTRRRAIVLAPGRGARAVLMERREREPSGPLATAAA"
     gene            complement(2140..3009)
                     /locus_tag="VF752_14835"
     CDS             complement(2140..3009)
                     /locus_tag="VF752_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8856869.1"
                     /translation="MREPNELRAQRADWENEAGVDSLQQFLNEARRYPLLTAAEEIEL
                     AKRIERGDMEAKDRMINSNLRLVVSIAKKYQGQGLSLGDLIQEGMLGLIRASEKFDWR
                     KGFKFSTYATLWIRQSIQRGLHNSARTIRLPVHIAQRERKISRLDRELSVKLGREPTD
                     EEIAEAAELPLDEVIEIRKAAQPTTSLDQTVSDDGDTALGDLLPADRPGPDEEVVDDM
                     RTAAVRRAIAGLPERERDVVELRFGVDGEPTPLRETGRLLGLSAERVRQLEERALAHL
                     AASGELDSFREAA"
     gene            complement(3243..3554)
                     /locus_tag="VF752_14840"
     CDS             complement(3243..3554)
                     /locus_tag="VF752_14840"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8856870.1"
                     /translation="MRDVHHPSREEIDLANVLHALSDPLRLEIVDILAETGEHNCSSL
                     GNGSISKSTLSHHLKVLREAGITWTRVEGTQRLVTLRSDDLGERFPGLIESVLSATKV
                     V"
     gene            3684..6119
                     /locus_tag="VF752_14845"
     CDS             3684..6119
                     /locus_tag="VF752_14845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8856871.1"
                     /translation="MRETRANRLGPIFESSEDAIVVAGPDGTIEDWNAAAERLYGHTD
                     EEAIGRPLTFLIPGDRAQERAEIFEMALAGTPVRNFETVRVRKDGSPVEVSLSAWPIR
                     GEGGRIEGVAAVAHDLSDVREAERRLRDREAQLSTAQEMAELGTWEWDVLTNEVTWTE
                     QLYRIYGLEPGEFGASYEAYMERVHPADREHVAARINAALQTLEPFSFLERIVRPSGE
                     VRTLQSRGRVVVDENGAAVRLIGVCQDVTRQVQDAERLRETAERLAEAQQIAHVGSWE
                     WDIENDVVTWSDELHRIYGVAREEFENDYSAYLKYVHPDDRERSEQTVRRALERREGF
                     EYEHRLVRRDGAVRVVLARGRAQTDENGRVVRMVGTAHDVTEQRQAQEQLARADAELE
                     LARRLQRVQRLTESALATLSLDELLPELAKRVDELLEVDNVAILVADEERAVLNQRVA
                     VGIEEDAGAIRIPFGQGFAGRVAAEERTIAVEDDAHRQVVGQLLRERKVRSLLGAPLI
                     AHGEVVGVLDVGSLRPRKFTDEEVRLLELAADRAALAVSHARLYERERQIAETLQRSL
                     LPEQLPDVGGVVLAARYLPAAGGADVGGDWYDAIPLPDGRLGVAMGDVTGHGVGAAAM
                     MGELRHALRAYAIEGIDPGEIAERLDKLIRLPGSERMATLLYMVLDPRSNRVEFVSAG
                     HLPPLVVRPDRQPSFLTVTGGLPLGCGMGPYEPGTAEIEPGSLLVLYTDGLVERRSKG
                     VDAGLEELAAMAPKIPFEPEAAADLILSTLLPEGPSLDDVAILTVRLVATAAERADVE
                     AAPETGAAPLVQD"
     gene            complement(6116..6886)
                     /locus_tag="VF752_14850"
     CDS             complement(6116..6886)
                     /locus_tag="VF752_14850"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8856872.1"
                     /translation="MAESFYVPDSEWFASTELTRGPWDRNAQHAGPPAALIGRAIEQL
                     GGDKRVGSVSLDILRPVPITRLRVEAHVLRSGRSVELVEASLSDEQGELVHARAWRIA
                     PGPEIEPVPGDPAPPGPAQSTEEPFFPIPWDVGYHSAMEWRFARGAFLEPGPATIWLR
                     MRHPLVPGESPTPLQRVLIAADTGNGASGPFDPSTHTFVNTDLSVHLFRAPEGEWVCL
                     DAVSLNDAHGLGLAESALYDERGRVGRGAQTLLVRPRA"
     gene            6949..8118
                     /locus_tag="VF752_14855"
     CDS             6949..8118
                     /locus_tag="VF752_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX8856873.1"
                     /translation="MGAIDELAVALRDRLLERAREAPDGERPLADDVRELVELEAGAL
                     SEGEQAELAERVVRLATGLGALDPLLADPEVDEVMVNGPRDVWVERRGRIEPAGVELG
                     GEAELRHAIERILAPLGRRVDEASPLCDGRLPDGSRVNVVIPPLAVDGPCLTIRRFRR
                     RGFSLDDLVANGTVPDPVAEFLARCVRARASVLVSGGTGSGKTTTLNALSGQIAAGER
                     IVTIEDAAELRLRQPHVVRLEARPPNLEGRGEVTVRQLVRNALRMRPDRIVVGEVRGA
                     EALDMLMALNTGHDGSLTTVHANSPEDALRRIETLALMADVGLPHAAVRKQVASAIAL
                     VVHQARADDGSRRVTAVSEVVRIVAGAATREVFALREGRALWRPPSGTELARRLE"
     gene            8115..8951
                     /locus_tag="VF752_14860"
     CDS             8115..8951
                     /locus_tag="VF752_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8856874.1"
                     /translation="MSAGLAFVAGCCGLLAAVDLFSLASGALPGVSRRVRPLLRVGDD
                     GRDPGVAERRRLLVVGAVLAFAAATFALGPIAGVAAGAAGPFAVSRLLEARRQRWRRR
                     LDTGAAAAALALADAIRGGHSLHGALLSAAPSMGGAAGTELRRCSLELQLGAPVDEAL
                     ERLRARAASRRYDTLVAACLLQRRAGGDLAALLRECARSFEDEERLLGDARAATAQAR
                     FTGLVVVMLPLGGAFLAELARPGVVASLAASALTAWLAGLALALQLAAAFWIRRLGRV
                     RA"
     gene            9087..9863
                     /locus_tag="VF752_14865"
     CDS             9087..9863
                     /locus_tag="VF752_14865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8856875.1"
                     /translation="MAVRAAARFQRPGEPVATVLGYHQVTDRGSHLAVSRRTFEEQMG
                     VLDEMRDELNPLPLNEIIARMARGTAPLRSVAVTFDDAWADVHENALGVLLEYRIPAT
                     LYVPTGYLDYPGFVSRSELQNMADAGIEIGAHTRTHPDLRGCTDAELESELAGSRADL
                     EDLLSRKVDAFAYPSGLHDKRVVAAVARAGFRSAVTTTRGWLRPASDLLRIRRSFVEE
                     FPLQTFTAGARGGMNVLKGTDALKAAMSRVSGRRPEGLPT"
     gene            complement(9808..11016)
                     /locus_tag="VF752_14870"
     CDS             complement(9808..11016)
                     /locus_tag="VF752_14870"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8856876.1"
                     /translation="MALNSDPRLEIEAHLRERTGRDCVYVPSGRFGLYVALRAWLTPG
                     DRILMSPVDDDVVLFTVLAAGLRPVMAPLSLADGNIDPDAVPDATWSSIRAVLTTNLY
                     GLPDRLPELRAHCDALGLVLAEDAAHALHTDVDGRPVGSWGEVSVFSLSKHAAGVGGV
                     IAFDDAARRDQLVRMRDDLVLPRSLRVRASDTLRPRAGAILERLGLRARMRARRAGER
                     RGSHRMPLRPRELEAAVRNAPGLEPFEPWIRVDKHDYRLEQRPQALARTVEHLRSLEQ
                     DRAARVRGVERLLELGVATGALRDGEPTALFRVPLIVEDRDTAARALEAAGHPFHYVY
                     DPPLDDYTGTAFSENSPAPEAARWWARHVLPVDPLLADPAIAALRALPDAVRSANPPD
                     AALRPGTSPP"
     gene            complement(11007..12218)
                     /locus_tag="VF752_14875"
     CDS             complement(11007..12218)
                     /locus_tag="VF752_14875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8856877.1"
                     /translation="MRVVAPERREADTPAVERIAPDAWQGIPDWDETVAAAAAPSIFL
                     TRDWASAWWASFGAGLEPCLLRVEHHDGRTAGLGLFYLDRLRGSPLTRLGMIGDRVVG
                     SEYLGLVARRGSEERVAGAVAAWLSAAARWDVADLGGLRPGDPAADALERSLSARARR
                     SHADEHPCATVALPGDFEEYLGGLNSKFRQRYRQRTNKLLRSCEVRFFLSERESDLPA
                     HLDTLFRLHQARWVEAGRPGVFADARMRAFYRDVATRLLRNGRLRFWQLEADGAIRAC
                     QFGFAYDGVLHSLQEAYDSDFAPPGVGGLGVVLRGHAIRAAIEEGLRAYDFLGGVEDH
                     KLRWGASVHQVRHVRIGSRGTRGALGWLASSGPEAAREWVKGALPDRVLEKLKAARAD
                     LHRRRGRREWR"
     gene            complement(12242..13216)
                     /locus_tag="VF752_14880"
     CDS             complement(12242..13216)
                     /locus_tag="VF752_14880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856878.1"
                     /translation="MTMMALRVSVIMPAYQSESTIGASISSVLWQTYDDVELVVADDG
                     STDGTRDIVKGFGDRVRFVERPHTGAAAARNAAISESTGQLLTFCDSDDVLFPNHIKT
                     LVDAHAGNRDIVTANALWMYPNGIVPGRTVHGRRIPAPEDQRMAILQRNFVSPMSIFS
                     RALIEEVGVFSEELEPAEDLAFWLEAIFAGYRVRLQPRPLALYRWSTSGVSGRREEVD
                     AAVARVLEQTARRPDLTDEERRYLDRRLAGDPPSVLVRRGDAAIRSGSYREAARLLGR
                     AARLMPVERPIVWKARLLSVAPGLSGRLLRARQRRLDRTEGVDQSFVR"
     gene            13288..14130
                     /locus_tag="VF752_14885"
     CDS             13288..14130
                     /locus_tag="VF752_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8856879.1"
                     /translation="MLDALLAFAAAGLGTAGAGLLVPAGARHGRNARLLRVAARVGRA
                     ARPLRRLAVPPDLRKRVSAAGLPPGLGIQEIMAAKVGAALLAAPLGAMLGAVAPGRLG
                     AALTVAAPVAGFLAPDRWLARRAAARARSARQELPAMLDLLRVTVHAGLPLTQALGEV
                     ARRSTGVVAAEWSTVARRVRLGVPMREALGTMAETLPIPETLALVAAVERSLKHGAPL
                     ADTLAAQAREARAARARAIREEAAKAGPKIQLVVALLLVPSVLLLVAAALGAALLGSG
                     AGLG"
     gene            14464..>14668
                     /locus_tag="VF752_14890"
     CDS             14464..>14668
                     /locus_tag="VF752_14890"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraZ N-terminal domain-containing protein"
                     /protein_id="HEX8856880.1"
                     /translation="MAFRGHFDYSLDAKNRLNVPPKFRAAFSGGVVLAKALEPCVAIW
                     TPEDFESFTESFLANLNPVSAERR"
BASE COUNT         2071 a         5073 c         5397 g         2127 t
ORIGIN      
        1 cgtcgcccgc ccgggagtgc cgctgatcgc cgcctgcgca ggttgcctga tcggagtgag
       61 gcataggtga gcgcggcctg ggcgacggtg atcgtggtgg gcgtcgccac gatcgtgatc
      121 aagggcaccg gcacgttcat cctgcacggc cggagcttgc ccgcctcgct cgaggggccg
      181 atcgcgatgc tggcgccggt gctgttggcg gcgctcgtcg caacgcaggc gttcggcgga
      241 gaccgagagc tggtcgtcga cgatcgcgcg cttggactgg gcgcagccgc cgtgctcgta
      301 gccctgcgtg cgcccaccct cgtggtggtg atcgccgcgg ctgccgtgac cgccctcgcg
      361 cgggctatct gaaatggtgc cgggcatgac gcgatttggg ctcatcggcc agttcctcgc
      421 ccagcccgga aagggcgacg cgctcgccgc gatcctgctc gaggccgcgc gcggactcga
      481 gtcgaacccc gactgcgagc tctacgcgat ccaccgctcg cccgatgagc ccgactcggt
      541 gtgggtgacc gaggtctgga agagccgcga ggcgcacggc gcgtcgctcg aggacccagg
      601 cgcgaaggag ctgatccagc aggcgaggcc gatcatcgcg ggcatcggcg agcggcgcga
      661 gctgcagccg ctcggcggga aggggatcgg ggactaggcc gcggcggtgg cgagcgggcc
      721 gctcggctcg cgttcgcgcc gctccatcag caccgcgcgg gctccgcggc ccggtgcaag
      781 cacgatcgcg cgccgccgcg tgggctcggc ctcggcccgc tccggctcga gcaccgcccg
      841 ggacgcgacg gcaccgagca ccaccttcat ctcgaacatg gcgaagctgg caccgagaca
      901 gcggcggatg ccgcctccga acgggatcca gccgtaggtg tcgggcgagc ggtcgaggaa
      961 gcgctcgggc cggaactcgt ccggctcggg atagaggtcc ggccggcggt gagtgaggta
     1021 gacgcacgga gcgaggaact cgccgcgagg aatctcgtag ccgtcaacct cgaaaggctc
     1081 gaccacgtgg cggccgacga tcgggatcgg cggccgcagc ctcagcgtct ccttgaccac
     1141 ggcgtcggca tagcgctcgt cgcgtacccg ctcgaatgag ccggctgtat gcaggagccg
     1201 ctcgaacgcc cacgccagcg ccgtggcagt ggtctcgtgg ccggcgagca gcaacgtcat
     1261 gagctcgtca cggagctcga catcggtcat cggctgcccg tcctcgtcgc gggccagcat
     1321 cagcatggaa aggatgtcgt cgcgctcctc gagatccggg tcacggcggc gttccgcgat
     1381 caagcggtag atcgcggcgt ccaccttgtc ccgcgcccgc cggaaggtcg catacggtcc
     1441 gaccggccag cggtcgtcgc gcagcacggg cacgagcgcc cagggccgcg tgctcacgtg
     1501 catcatgcgc ttgaggagcc gccgcacgtc ctcagtacgc gcgctctcct cgaggccgaa
     1561 gatcacgcgc aggatcacat cgagcgtgat gtcctgcatc gacggctgaa gccggaacgg
     1621 ccgcccgatc ggccaggagt cgatcacgcg gtcggtgatg ccgcgcatca gctcctcgta
     1681 gcgcacgagc cgctcgccgt ggaacggcgg cagcatcaac ttgcgctggc gcaggtgctc
     1741 ggcgccgtcc agcagcagca ccgagtgctc gccaagcaca ggctcgagcg tgaagttcgc
     1801 ctcgccggcg cgcaggaccc cgcgatcggc cgtgaagatg cgcttgagct cgtccggatc
     1861 ccagacgaac acgagcctgc cgatctgcgc cagccgcacg gtgaacgcct ctccgtacag
     1921 cgcggccgac tcacgcatga acgcctccgg ccggacgatc cagttgagcg tctgaacggc
     1981 tgcgggcatg cgcggcccgg gtgggaggct catggagaga atctaccgcc gcgtccagcc
     2041 ggcaccgagc cgcagcgccg tcccggcgcc gcggctcggc gggagtgccg gcggacacgc
     2101 tgacctcagg ggagaggcct gcggtcgcgc gagagcggct taggccgctt cgcggaagct
     2161 gtcgagctcg ccgctcgcag cgaggtgcgc gagagcacgc tcctcgagct ggcggacccg
     2221 ctcggcagag agtccgagca gtcgcccggt ctcacggaga ggtgtgggct caccgtcgac
     2281 gccgaagcga agctcgacca cgtcgcgctc acgctctggc aggccggcga tcgcacgacg
     2341 gacggcggcc gtgcgcatgt cgtccacgac ctcctcgtcc ggacccggac ggtcggcggg
     2401 gagcaggtcg ccgagtgccg tgtcgccgtc gtcggagacg gtctggtcga ggctcgtggt
     2461 cggctgagca gccttccgga tctcgatcac ctcgtcgagc ggaagctccg cggcctctgc
     2521 gatctcctcg tcggtcggct cacggccgag cttgacggag agctcgcggt cgaggcgcga
     2581 gatcttgcgc tcgcgctgcg cgatgtgcac cggcaggcga atcgttcgcg ccgagttgtg
     2641 cagaccgcgc tggatcgact gccggatcca cagcgtcgcg tacgtcgaga acttgaagcc
     2701 ctttcgccag tcgaacttct cactggcgcg aatcaggccg agcatgccct cctggatcag
     2761 gtcacccagg ctcaggccct ggccctggta cttcttcgcg atggagacga ccagccgcag
     2821 gttcgagttg atcatgcggt ccttggcctc catgtccccg cgctcgatcc gcttcgccag
     2881 ctcgatctcc tcggcggctg tgagcagggg gtaacgccgc gcctcgttca ggaactgctg
     2941 aagcgagtcg acgcccgcct cgttctccca atcggcgcgc tgagcgcgga gctcatttgg
     3001 ctccctcacg gccttacggg ccgtcgcgat gttgctcttc ttcgtcaggg tgctccttct
     3061 tggaaagttc gatggacttg aaatattagc agaatttatt gctcgcgcaa ccgtcttaac
     3121 cagttcttta tagggcggga cggccggcgg ccgctttctc cgggggtagg cgcaatgggt
     3181 gcagatacca cacgcgcacg cgccgtactc ctataacccc cggtgcgtcc ggtggttccg
     3241 gtttagacga ccttcgtcgc gctgagcacc gattcgatga ggcctgggaa gcgctcaccg
     3301 agatcgtcgc ttcgaagcgt cacgagccgc tgggttcctt ctacgcgggt ccaggtgatt
     3361 cctgcctccc gcaacacctt gaggtggtgc gagagggtcg atttcgagat gctcccattc
     3421 ccgagggagc tgcagttgtg ctcgccggtc tcagccagga tgtccacgat ttccaggcgc
     3481 agcgggtcgc tcaacgcgtg caggacgttc gcgagatcga tctcctcgcg gcttggatgg
     3541 tggacatctc tcactgtgcg gcgggccctt cgttcgactc ctgacgaact accgaatcaa
     3601 tatagcggcg gttcccggat ctgacgaccg caagggcgat cgcagagtgg tattgcgcca
     3661 cggcgggtaa tacgctccgc ctaatgaggg agacgcgggc gaatcgcctc gggcccatct
     3721 tcgagtcgtc cgaggacgcg atcgtcgtgg cggggccgga cgggacgatc gaggactgga
     3781 acgccgctgc ggagcgcctc tacggccaca ccgacgagga ggcgatcggg cgcccgctca
     3841 ccttcctgat cccaggcgat cgcgcccagg agcgcgcgga gatattcgag atggcgctgg
     3901 cgggcacgcc ggtccgcaac ttcgagaccg tgcgggtgcg caaggacggc tcgccggtcg
     3961 aggtatcgct gagcgcgtgg ccgatccgcg gcgaaggcgg gcggatcgaa ggcgttgcgg
     4021 ccgtcgccca cgacctgagc gacgtgcgcg aggccgagcg gcgcctacgc gaccgggagg
     4081 cgcagctctc caccgcccag gagatggccg agctcggcac gtgggaatgg gacgtgctga
     4141 cgaacgaagt gacctggact gagcagctct accgcatcta cgggctcgag ccgggagagt
     4201 tcggagcctc atacgaggcg tacatggagc gggttcaccc cgccgaccgc gagcacgttg
     4261 ccgcccgcat caacgccgcg ctccagacgc tcgagccgtt ctcgttcctc gagcggatcg
     4321 tgcggccgag cggcgaggtg cgcacgctgc agtcccgcgg gcgcgtggtc gtggacgaga
     4381 acggcgccgc ggtgcggctg atcggggtct gccaggacgt gacacgccag gtgcaggacg
     4441 ccgagcggct tcgcgagacc gccgagcggc tggccgaggc ccagcagatc gcgcacgtcg
     4501 gcagctggga gtgggacatc gagaacgacg tggtcacctg gtccgacgag ctccaccgca
     4561 tctacggcgt cgcgcgtgag gagttcgaga acgactacag cgcgtatctg aagtacgtcc
     4621 acccggacga ccgcgagcgc tcagagcaga cggtgcggcg ggcgttagag cgccgcgagg
     4681 gcttcgagta cgagcaccgg ctggtgcgcc gcgacggcgc cgtgcgcgtg gtgctggcgc
     4741 gcgggcgcgc gcagaccgac gagaacggcc gggtcgtgcg gatggtgggc acggcgcacg
     4801 acgtgaccga gcagcgccag gcccaggagc agctggcacg cgcggacgcg gagctcgagc
     4861 ttgcgcggcg gctccagcgc gtgcagcggc tcacggagtc ggcgctcgcg acgctgagcc
     4921 tcgacgagct gctgcccgag ctcgccaagc gcgtggacga gctgctcgag gtcgacaacg
     4981 tcgcgattct cgtcgccgac gaggagcgcg cggtgctgaa ccagcgcgtc gcggtgggga
     5041 tcgaggagga cgccggagca atccggatcc cgttcgggca gggcttcgcc ggccgggttg
     5101 ccgccgagga gcgcaccatc gccgtcgaag acgatgccca ccggcaagtt gtcgggcagc
     5161 tcctcaggga gcgcaaggtg cgctcgctgc tcggcgcgcc cctgatcgct cacggcgagg
     5221 tggtcggcgt gctggacgtt gggtcgctca ggccccgcaa gttcaccgac gaggaggtgc
     5281 gactgctcga gcttgcggct gatcgcgccg cgcttgccgt ttcacacgcg aggctctacg
     5341 agcgcgagcg gcagatcgcc gagacgctcc agcggagcct gctgcccgag cagcttccgg
     5401 acgtgggggg cgttgtgctc gcggcccgct acctgccggc agccggcggc gccgacgtgg
     5461 gcggcgactg gtacgacgcg atcccgctac ccgacggccg cctgggcgtt gcgatgggcg
     5521 acgtgaccgg ccatggcgtc ggtgcggccg ccatgatggg cgagcttcgc cacgcgcttc
     5581 gcgcctacgc gatcgagggc atcgacccag gggagatcgc cgagcggctc gacaagctga
     5641 tccggctgcc cggcagcgag cgcatggcca cgctcctcta catggttctc gacccgcgct
     5701 cgaaccgagt cgagttcgtg agcgccgggc acctgccgcc tctcgtcgtc cgccccgaca
     5761 ggcagccgag cttcctcacc gtcaccggcg ggctgccgct cggctgcggc atggggccgt
     5821 acgagcccgg gacagccgag atcgagcccg gctccctgct cgtgctctac accgatgggc
     5881 tggtcgagcg gcgctcgaag ggcgtcgacg ccggcctcga ggaactcgcc gccatggcgc
     5941 cgaaaatccc gttcgagccg gaagcggccg ccgacctcat tctgagcaca ctcctgcccg
     6001 agggcccgtc gctcgacgac gtcgcgatcc tcaccgtgcg tctcgtcgcc acggccgcag
     6061 agcgcgcaga cgtcgaggct gcgcccgaaa ccggtgcggc gccgctcgtc caggactagg
     6121 cgcgcggcct gaccagcagc gtctgggccc cgcggcccac gcgcccgcgc tcgtcgtaga
     6181 gcgcgctctc ggcgaggccg agcccgtgcg cgtcgttgag gctgaccgcg tcgaggcaca
     6241 cccactcgcc ctcgggcgcg cgaaagaggt gcacggacag gtcggtgttc acgaacgtat
     6301 gcgtgctggg gtcgaacggg ccgctagcgc cgttccccgt atccgccgcg atcaggaccc
     6361 gctggagcgg ggtggggctc tcgccgggaa cgagcgggtg gcgcatgcga agccagatcg
     6421 tcgcggggcc cggctcgagg aaggcgccgc gcgcgaagcg ccactccatc gcgctgtggt
     6481 agccgacgtc ccaggggatc gggaagaacg gctcctccgt gctctgggcg ggccctggcg
     6541 gcgccggatc gccaggcacg ggctcgatct ctggaccggg tgcgatgcgc caggcccgcg
     6601 cgtgcaccag ctcgccttgc tcgtcggaca gcgaggcctc tacgagctcg acgctccggc
     6661 cggaccgcag cacgtgcgcc tccacccgca gccgggtgat cgggacgggt cgcagtatgt
     6721 cgagcgacac cgatcccacc cgcttgtcgc cgcctagctg ctcgatcgcg cggccgatga
     6781 gcgctgcagg cggtccggcg tgctgcgcgt tgcggtccca agggccgcgt gtcagctccg
     6841 tcgaggcgaa ccactcgctg tccggcacgt agaaggactc cgccatggcg ggcggcatcc
     6901 tctcagccgc ggggggcgaa agccgtgcgg cgcggtgctt gtcccgtcat gggagcgatc
     6961 gacgagctgg cggttgcgct tcgggacaga ctgctcgagc gggcgcggga ggcgcccgac
     7021 ggcgagcgtc cgctggcaga cgatgtgcgc gagctggtgg agttggaggc gggcgcgcta
     7081 tccgagggag agcaggcaga gctggccgag cgggtggtgc ggctcgccac cgggctcggc
     7141 gcgctcgacc cgctgctggc ggaccctgag gtggacgagg tgatggtcaa cggcccgcgc
     7201 gacgtctggg tcgagcgccg cggacggatc gagccggccg gcgtggagct cggcggcgaa
     7261 gccgagctgc gccacgcgat cgaacggatc ctcgcgccgc tcggcaggcg cgtggacgag
     7321 gcctcgccac tctgcgacgg ccgcctgccc gatggctcgc gcgtgaatgt ggtgatcccg
     7381 ccactggccg tcgacggtcc gtgcctcacg atccggcgct tccgccggcg cggcttctcg
     7441 ctcgacgacc tggtcgcgaa cggcaccgtg cccgatccgg tcgccgagtt cctcgcgcgt
     7501 tgcgtgcgcg cgcgggcctc ggtgctcgtc tccgggggta ccggatcggg caagaccacg
     7561 accctgaacg cgctctccgg ccagatcgcg gcgggcgagc ggatcgtcac gatcgaggac
     7621 gcggcagagc tgcggctgcg ccagccgcac gtcgttcggc tcgaggcgcg cccgccgaac
     7681 ctggagggga ggggagaggt gacggtgcgt cagctggtgc gaaacgcgct ccgcatgcgg
     7741 cccgaccgga tcgtcgtggg cgaggtgcgc ggcgccgagg cgctggacat gcttatggcg
     7801 ctcaacactg ggcacgacgg ctcgctcacc accgtccatg ccaactcacc ggaggacgcg
     7861 ctgcgccgga tcgagacgct cgcgctgatg gcggacgtcg gattgccgca cgccgcggtg
     7921 cgcaagcagg tggcgtcggc gatcgcccta gtggtgcacc aggcgcgcgc cgacgacggg
     7981 agccggcgcg tgacggcggt gtccgaggtg gtgcggatcg tggcgggcgc ggccacgcgc
     8041 gaggtgttcg cgctgcgcga ggggcgcgcg ctgtggcggc cgccgtcggg caccgagctc
     8101 gcgcgccggc tcgagtgagc gccggcctgg cgttcgtcgc cggctgctgt ggtctcctgg
     8161 cagccgtgga tctcttctcg cttgcatctg gcgctctgcc aggcgtctca cgccgcgtgc
     8221 gaccgctgct ccgggttggc gatgacgggc gcgatcccgg tgttgctgag cggcggcgcc
     8281 tgctggtagt gggcgcggtg ctcgcgttcg ctgcagccac ctttgcgctc gggccgatcg
     8341 ccggcgtcgc cgccggggca gctgggccgt tcgctgtctc gcgtctgctg gaggcgcgcc
     8401 ggcagcgctg gcgccgccgg ctcgatacgg gcgccgcggc ggcggcgctg gcgctggcag
     8461 acgccatccg cggcggccac tcgctccacg gagcgctgct gtccgcagcg ccctccatgg
     8521 gcggggcggc gggcacggag ctccggcgct gctcgctcga gctccagctg ggtgcgcccg
     8581 tcgatgaggc gctcgagcgc ctgcgcgcgc gggcggcgag ccggcgctac gacacgctcg
     8641 tggccgcgtg cctgctgcag cgccgcgccg gcggcgatct cgcagcgctg ctgcgtgagt
     8701 gcgctcgctc gttcgaggac gaggagcggc tgttgggcga tgctcgcgcg gccacggcgc
     8761 aggcccgctt caccgggctc gtggtggtga tgctgccgct cggcggggca ttcctggccg
     8821 agctggctcg cccgggtgtc gtcgcctcgc tggccgcttc agcgctgacc gcctggcttg
     8881 ccggtttggc gctcgccctg cagctcgcgg cggcgttctg gatccggcgg ctgggccgcg
     8941 tgcgcgcctg atcggggtga ccggtttggg gtggctcaaa cccggtacac caaaccgctg
     9001 gaggcgagag tgctggtcga agcaaaagct ccggcccgcg gacgcccgat ggaagcggcc
     9061 gcgggcagat gagacaggcc tttcagatgg cggttcgcgc agccgcccgc ttccagcggc
     9121 caggcgagcc agtggccacg gtgctcggct accaccaggt gacggacagg ggtagccatc
     9181 tggcggtgag ccgccggacc ttcgaggagc agatgggcgt cctcgacgag atgcgggacg
     9241 agctcaatcc gctgccgctc aacgagatca tcgccaggat ggctcggggc accgcgccgc
     9301 ttcgctcggt cgccgtcacg ttcgacgacg cctgggccga cgtccacgag aacgcgctcg
     9361 gcgtgctgct cgagtaccgg attccggcga cgctctacgt ccccaccggc tacctcgact
     9421 acccgggttt cgtgagccgt tcggagctgc agaacatggc tgacgcgggg atagagatcg
     9481 gcgcccacac gcgcacgcac ccggacctga ggggctgcac cgacgccgag ctcgaatccg
     9541 agctcgcggg cagccgcgcc gacctggagg acctgctttc gcgcaaggtc gacgcgtttg
     9601 cctatccctc cggcctccac gacaagcgcg tggtcgccgc ggtggcgcgg gccggcttcc
     9661 ggtcagccgt cactacgacg cgcgggtggc ttcgcccggc gtcggacctc ctgcgcatac
     9721 ggcgcagctt cgtggaggag ttcccgcttc agaccttcac cgcgggagcg cgtggaggaa
     9781 tgaacgttct caaaggcacc gacgccctca aggcggcgat gtcccgggtc tcagggcggc
     9841 gtccggaggg tttgccgacc tgaccgcgtc aggcagcgcg cgcagggcgg cgatggccgg
     9901 gtcggcgaga agcgggtcca ccgggagcac gtgacgcgcc caccagcggg cagcctcggg
     9961 agcgggggag ttctctgaga acgcggtgcc cgtgtagtcg tcgagcggcg ggtcgtacac
    10021 gtagtggaag ggatggcccg ccgcctcgag cgcacgcgcg gccgtgtcgc ggtcctccac
    10081 gatgagcggc acgcgaaaca gggcggtcgg ttcgccgtcc cggagcgcgc ccgtcgcgac
    10141 gccgagctcg agcagccgct cgaccccacg cacgcgcgcg gcgcggtcct gttcgagcga
    10201 gcgcaggtgc tccacggtgc gcgcgagcgc ctgcgggcgc tgctcgagcc ggtagtcgtg
    10261 cttgtcaaca cgtatccagg gctcgaacgg ctcgagcccg ggagcgttcc ggaccgccgc
    10321 ctcgagctct cgcggtcgca gcggcatgcg gtgggagccg cgccgctcgc cggcccgacg
    10381 cgcgcgcatc cgcgccctca gccctagtcg ctcgagaatg gcgcccgcgc gcggccgcag
    10441 cgtgtccgac gcacggactc tcagggatct cggcagcacc aggtcgtcgc gcatgcggac
    10501 gagctgatcg cggcgggcag cgtcgtcgaa cgcgatcacg ccgccaacgc cggcggcgtg
    10561 cttcgaaagg ctgaacaccg acacctcgcc ccacgacccg accggtcgcc cgtcgacgtc
    10621 cgtgtgcagc gcatgagccg cgtcctcggc gagcacaaga ccgagcgcgt cgcaatgcgc
    10681 gcgtagctcg ggaaggcggt ctgggaggcc gtacaggttc gtggtgagca cggcccggat
    10741 cgacgaccag gtggcgtcgg gaacggcgtc cggatcgatg ttgccgtcgg cgagcgagag
    10801 cggcgccata acgggccgca ggcctgcggc gagcacggtg aacaggacca cgtcgtcgtc
    10861 gaccggtgac atgaggatgc ggtctcccgg ggtgagccag gcgcgaagcg cgacgtacag
    10921 cccgaaccgg cccgagggca cgtacacgca gtctcgcccg gtccgctcgc gcaggtgggc
    10981 ctcgatctcg agccgcggat cgctgttcag cgccattcgc ggcggcccct ccgccggtgg
    11041 agatcggcgc gagcggcctt cagcttctcg aggacccgat cgggaagcgc gcccttgacc
    11101 cactcgcgcg ccgcctccgg cccggacgat gcgagccagc cgagggcgcc cctggtcccc
    11161 cggcttccga tgcgcacgtg ccgcacctga tgaaccgagg cgccccagcg gagcttgtgg
    11221 tcctccacgc cgccgaggaa gtcgtaggcg cgcagccctt cctcgatcgc cgcccggatc
    11281 gcgtggccgc gcagcaccac gccgagcccg cccacgcctg gtggagcgaa gtcgctgtcg
    11341 taagcctcct gcagcgaatg cagcacgccg tcgtacgcga agccgaactg gcacgcccgg
    11401 atcgcgccgt cggcctcgag ctgccagaag cgcaggcgcc cgttgcgcag caggcgcgtc
    11461 gcgacgtcgc ggtagaacgc gcgcatgcgc gcgtccgcaa acacgcccgg gcgtcccgcc
    11521 tcgacccagc gcgcctggtg caggcggaag agcgtgtcga ggtgcgcggg caggtcggac
    11581 tcccgctcgc tcaggaagaa gcgcacctcg cacgagcgga gcagcttgtt ggtgcgctgg
    11641 cggtaccgct ggcggaactt cgagttgagc ccgccgaggt actcctcgaa gtcgccgggg
    11701 agcgcgacgg tcgcgcatgg gtgctcgtcc gcgtgggagc gccttgctcg tgcggagagc
    11761 gagcgctcga gcgcgtcagc cgcggggtcg ccggggcgca ggccgccgag atcggcgaca
    11821 tcccagcgcg cggctgccga gagccatgcg gcgaccgcgc ccgccacgcg ctcctcgctg
    11881 ccgcgacgcg cgacgaggcc gaggtactcg gagccgacca cgcggtcgcc gatcatgccg
    11941 agacgcgtga gcggcgagcc tcgcaggcgg tcgagataga acagaccgag cccagcggtg
    12001 cgcccgtcgt ggtgctcgac ccgaagcagg cacggctcga ggccggcgcc gaagctcgcc
    12061 caccaggcgc tcgcccagtc acgcgtgagg aaaatgctcg gcgcggctgc ggcggccacc
    12121 gtctcatccc aatccggaat tccctgccac gcgtccggcg cgatccgctc gactgcgggc
    12181 gtgtccgctt cgcgacgctc aggagcgacg acacgcatca tggattgagg ctacccctgt
    12241 ctcatcggac gaagctctgg tcgacgccct cggtgcggtc gagccggcgc tgccgcgcgc
    12301 gcaacaggcg gcccgagagg ccaggcgcca ccgagagcag ccgagccttc cagacgatcg
    12361 gtcgctcgac gggcatgagt cgcgcagccc ggccgagcag tcgagcggct tcgcggtagg
    12421 acccgctgcg gatcgcggcg tcgccccggc gcacgagcac cgagggtggg tcgccggcga
    12481 gccgccggtc gaggtagcgg cgctcctcgt ccgtgaggtc cgggcgccgc gcagtttgct
    12541 cgagcacgcg cgcgaccgcg gcgtcgacct cctcacggcg tccgctgacg ccgctcgtgc
    12601 tccaccggta gagcgcgagc gggcgcggct gaagccgcac gcggtacccg gcgaagattg
    12661 cctcgagcca gaaagcgagg tcctcggcgg gttccagctc ctcgctgaag acacccacct
    12721 cttcgatcag cgcgcgcgag aagatcgaca tgggggagac gaagttgcgc tgcaggatcg
    12781 ccatgcgctg atcctccggc gcgggaatgc ggcgcccgtg caccgtcctc ccggggacga
    12841 tgccgttcgg gtacatccag agggcgttgg cggtgacgat gtcgcggttg cccgcgtgcg
    12901 cgtccacgag cgtcttgatg tggttgggaa agagcacgtc gtcggagtca cagaacgtga
    12961 gaagctgccc agtcgactcg ctgatcgccg cgtttcgcgc ggcggccgct ccggtgtgtg
    13021 gccgctcgac gaagcgcacg cggtccccga agcccttgac gatgtcgcgc gtgccgtcgg
    13081 tcgagccgtc gtcggccaca accagctcga cgtcgtcgta ggtctgccag agcaccgacg
    13141 agatcgacgc cccgatcgtc gactccgact ggtaggccgg catgatgacg ctcacccgca
    13201 gagccatcat ggtcatgatg gatcagcttc gcccgtttgt cgcatctcgg ccggggcgag
    13261 accgcctgcg gcgcggctta acctgacatg ctcgacgcgt tgctggcgtt tgccgccgcc
    13321 ggcctcggga cggccggcgc gggcctgctc gttccggccg gcgcccggca cggccgcaac
    13381 gcccggctgc tgagggttgc cgcgagggtc ggaagagcag cgcggccgct tcggcggctc
    13441 gcggtgccgc cggacctgcg caagcgcgtg agcgcggcgg gtctgccgcc cgggctgggg
    13501 attcaggaga tcatggccgc gaaggtcggc gcggcgctgc ttgcggcccc gctcggagcg
    13561 atgctcgggg ccgtcgcccc gggccggttg ggagcagcgc tcaccgtcgc ggcgcccgtc
    13621 gcgggtttcc tcgcgcccga tcgctggctt gcccgccggg ccgcggcgcg tgcgcggagt
    13681 gcgcggcaag agctgccggc gatgctcgac ctcctacgcg tgacggtcca cgcgggcctg
    13741 ccgctcacgc aggcgctggg ggaggtggcg cggcgctcga cgggtgtggt cgccgcggag
    13801 tggtcgacgg tcgcgcgtcg cgtccggctc ggtgtgccga tgcgcgaggc gctcgggacg
    13861 atggccgaga ccctgcccat cccggagacg ctcgcgctcg tggctgcggt cgagcgctcg
    13921 ctcaagcacg gtgcgccgct cgcagacacg ctcgccgccc aggcgcgaga ggctcgcgcc
    13981 gcccgcgccc gcgcgattcg cgaggaggcc gcgaaggcgg gcccgaagat ccagctcgtg
    14041 gtggcgctgc tgctcgtgcc gtccgtgctg ctgctcgtgg cggccgcgct cggggcggct
    14101 ctactcggtt cgggcgcagg cctcggctga gcccccacga cgccccaccg gggcgcaaga
    14161 acccgtgcag gggacgcacc gttcttgcaa gcacgcgtgc agttggcgcg attgcaacgg
    14221 tcgtaccggt gcctgcgagc gctgttgcgc cgattcgtcc caccacgctt tcccgctcca
    14281 ggagcgggat cgaaggctct gggcgggtct tcggtgggcc gtcaagggga cctcgtggag
    14341 aaatgtgttg caaagtgggg aggtgtgggg tagattcgcc ccatcaaacg gaccggagtg
    14401 gcggggctcc tcccactccg ccactccggt ccagatcttc ttcgacccca cctgagatga
    14461 gagttggcgt tccgcggcca cttcgactac tcgctagacg ccaagaaccg gctgaacgtg
    14521 ccgcccaagt tcagagccgc gttctcaggt ggagtggttc tcgcgaaggc gctcgagccc
    14581 tgcgtggcga tctggacgcc ggaggacttc gagagcttca cggagtcgtt tctcgcgaac
    14641 ctgaacccgg tgagcgccga gcgccgca
//
LOCUS       DASVXS010000260         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_824666, whole genome shotgun sequence.
ACCESSION   DASVXS010000260 DASVXS010000000
VERSION     DASVXS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824666"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="VF752_14895"
     CDS             <1..1794
                     /locus_tag="VF752_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX8856881.1"
                     /translation="LNVGVLPHHRNWGDVLANLAWVVVDEAHVYRGVFGSHVGNVLRR
                     LRRLANAYGTEPRFILTSATIANPVELAEKLTGLDFRLVDRDGAPASERQIAMWNPPL
                     LDEDSGERVSALSEAAQLLTELVQHEVRTICFLNSRRGVELIQKFTRLRLEDAGRSDL
                     ADRIAPYRAGYTPAQRREIERRLAEGELLAVAATNALELGIDIGDLDAAICVTFPGTV
                     ASLRQMWGRAGRRNTGLALYVAGDDALDQFFCRHPDEFLDRAVESAILDHESEEIHLP
                     HLVAAAYEMPLSADDAETLGPRWEAYAERLVKIGELRERNGRYMPRGAGFPAGQLSLR
                     SSSLDAFAVVDAERGEIIGNVEAARAPATLHEGAVYLHMGQSFEVEELDLHNRRAIVR
                     QFNGDWYTQPKKETDTWIEQVIARRSVQGVELSFGYVSVTEQVVAFQRKRVSDHEVLD
                     LLPVDLPEQRFVTQALWYELPEPLLREEFPLDVLQGSLHAAEHGQIAVLPLIAMCDRW
                     DIGGLSTAFHAQTGRPTIFIYDGHPGGVGITRVGYEAFANLVDDARRLIAECPCKSGC
                     PSCVQSPKCGNLNEPLNKHGALEILTRMQAA"
     gene            1894..>2394
                     /locus_tag="VF752_14900"
     CDS             1894..>2394
                     /locus_tag="VF752_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856882.1"
                     /translation="MAPSVASAASGSGGVTAPDTGNAPAASQPQSGNGGATFGDDSLA
                     PEPAPKPKPKSKPRSKRAPRPSGRPLLTLFQVGPSSLFAYGPPGQVKFAIRDRSAIVR
                     VKLQVISKASRKIVRTLDLGDRATGSVQTFLLSGGTLPEGSFELKLAARDPSGRRLRS
                     SANASNV"
BASE COUNT          371 a          846 c          830 g          347 t
ORIGIN      
        1 ctgaacgtgg gagtgctccc gcaccaccgc aactgggggg acgtgctcgc gaacctcgcg
       61 tgggtagtgg tcgacgaggc gcacgtgtac cgcggcgtgt tcggctcgca tgtgggcaac
      121 gtgttgcgcc gtctccggcg actcgcgaat gcctacggca cggagccgcg cttcatcctg
      181 acgagcgcaa cgatcgccaa cccggttgag ctcgccgaga agctcaccgg gctcgacttc
      241 cggctcgtgg atcgcgacgg agcaccggcg tcggagcggc agatcgcgat gtggaacccg
      301 ccgctgcttg acgaggactc gggcgagcgg gtctcggcgc tgtcggaggc ggcccagctt
      361 ctcaccgagc tcgtgcagca cgaagtgcga acaatctgct tcctcaactc gcgccgcggc
      421 gtggagctga tccagaagtt cacgcgcctg cggctcgaag acgcaggacg gtctgacctg
      481 gccgaccgga tcgctcccta ccgagcgggc tacacgccgg cgcagcgccg cgagatcgag
      541 cgccgcctgg ctgagggcga gttgcttgcc gtggccgcga ccaacgccct cgagctcggc
      601 atcgacattg gcgacctcga cgcggcgatc tgcgtcacgt tccctggcac cgtcgcgagc
      661 ctgcgacaga tgtggggccg cgccgggcgc cgcaacaccg gcctcgcgct ctacgtcgcc
      721 ggcgacgacg cgctcgacca gttcttctgc cgccacccgg acgagttcct cgaccgcgcc
      781 gtcgagtcgg cgatcctcga ccacgagtca gaagagatcc acctgccgca cctcgtcgca
      841 gccgcctacg agatgccgct ctccgcggac gacgccgaga ccctcggccc gcgctgggag
      901 gcgtacgcgg aacgactggt gaagatcggc gagctacgcg agcgaaacgg gcgctacatg
      961 ccgcgcggcg cgggcttccc cgccggccag ctctcgctga ggtcgtcgtc gctcgacgcg
     1021 ttcgccgtag tcgacgcgga acgcggcgag atcatcggca acgtcgaggc ggcgcgcgcg
     1081 ccggccaccc tccacgaggg cgccgtctac ctgcacatgg ggcagagctt cgaggtcgag
     1141 gagctcgacc tgcacaaccg gcgcgcgatc gtccggcagt tcaacggcga ctggtacacg
     1201 cagccgaaga aggagaccga cacctggatc gagcaggtga tcgcgcgccg ctccgtgcag
     1261 ggcgtggagc tgtcgttcgg ctacgtgtcg gtcaccgagc aggtggtcgc gttccagcgc
     1321 aagcgcgtat cggaccacga ggtgctcgac ctgctgccgg tcgacctgcc cgagcagcgc
     1381 ttcgtgaccc aggcgctctg gtacgagctg ccggagccgc tccttcgcga ggagttcccg
     1441 ctcgacgtgc tccagggttc gctgcacgcc gcggagcacg gacagatcgc ggtgctgccg
     1501 ctgatcgcga tgtgcgaccg atgggacatc ggcggactct cgaccgcgtt ccacgcgcag
     1561 accggccggc cgacgatctt catctacgac ggtcatcccg gtggcgtcgg gatcacgcgc
     1621 gtgggatacg aggcattcgc gaacctggtg gacgatgcgc gccggctgat cgccgagtgc
     1681 ccctgcaagt ccggctgccc gtcgtgcgtg cagtcgccga agtgcggcaa cttgaacgag
     1741 ccgctgaaca agcacggcgc gctcgagata ctcacgcgca tgcaggccgc ctgagcggct
     1801 gcaggatccg ttgcacgccc tcagcgcgtc gcgctccgcg ccgaccgcag aggtgtgcga
     1861 tccctcctcg ccacggccct cgcggcctcg ctgatggcgc cctccgtcgc gtccgctgcc
     1921 agcggcagcg gaggtgtgac cgcgccggac accggaaacg cgcctgcggc cagtcagccg
     1981 cagagcggaa acggcggcgc caccttcggc gacgacagcc tggcgcccga gcctgcgccg
     2041 aagccgaagc cgaagtccaa gccgcgttcg aagcgagcgc cgcgcccgag cgggcgcccg
     2101 ctgctcacgc tgttccaagt ggggccgtcg agcctgttcg cgtacggtcc gccggggcaa
     2161 gtgaagttcg cgatccgcga ccgctcagcc atcgtccgcg tgaagctgca ggtgatctcg
     2221 aaggcctcgc gcaagatcgt gcgcacgctc gacctcggcg accgcgcgac cggttcggtc
     2281 cagaccttct tgctcagtgg tgggacgctg ccggaggggt cgtttgagct gaagctggcg
     2341 gcgcgtgacc cgagcggccg gcgactgcgc tcgagcgcca acgccagcaa cgtg
//
LOCUS       DASVXS010000261         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_753492, whole genome shotgun sequence.
ACCESSION   DASVXS010000261 DASVXS010000000
VERSION     DASVXS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753492"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1701
                     /locus_tag="VF752_14905"
     CDS             247..1701
                     /locus_tag="VF752_14905"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX8856883.1"
                     /translation="MERAEAEAIYDSGREACVEFILDLAGRVERHEERLARLEAQLRQ
                     DSRSSSKPPSSDPPKTRAQRRAEARAKAKELMRREGERRTAGGQSGHRGAGRELRPED
                     QVDEIVDHYPGACGGCGRRFAAEQRRPSRRFGRHQIAELPAISVRWTEHRTHQLRCRH
                     CRARTSARLPEPLGACAFGPRLQAAIVTLTGRHRISRRGITELARDLFGVTLATGSVD
                     AICQRASEALAGPHLQLHDWVLEQGSLHVDETGWRTAGEGRALWAATTPKAAFFEIAE
                     HCNREQFTALIGPYPGIVVSDRWNGYAHLDPHQRQVCWSHLQRDFRRHADGLGEQKTF
                     GEQGFPLTKQVFAAWRAYQHDHHDRDRLQAEIAPIQTQLRQLLEAASPKSRRNRWHRQ
                     FATNLLKVWPALWTFTTVDGVEPTNNPAERALRGPVIHRKLSLGTQSHAGERFAERVL
                     STATTCRLQGRSLFTYLSDLITAHNRGDPFPALA"
     gene            complement(1752..1825)
                     /locus_tag="VF752_14910"
     tRNA            complement(1752..1825)
                     /locus_tag="VF752_14910"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1789..1791),aa:Met,seq:cat)
     gene            complement(1934..2056)
                     /locus_tag="VF752_14915"
     CDS             complement(1934..2056)
                     /locus_tag="VF752_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856884.1"
                     /translation="MAAQPQPQPSPPKPPPEPLPQTIFFTEKRGDTPADPPRER"
     gene            complement(2061..>2359)
                     /locus_tag="VF752_14920"
     CDS             complement(2061..>2359)
                     /locus_tag="VF752_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856885.1"
                     /translation="VVGLLGAVILVLAGAYVLHPRRLAFSVNASATLAAIRGRDAEIL
                     EDQDLYHETMIATLTRRREGNQPIVDRLHTAFSVGLLGLLAELSGLALAAALAS"
BASE COUNT          385 a          798 c          814 g          362 t
ORIGIN      
        1 accgagacgc gaaaacgccc ttcacgtcgg cgaaggctcg gctcatgaag ggctgagaag
       61 ccggccattt ccagggaaag accggagagc ggctgaggga ctcgaaccct cgaccttctg
      121 catggcaagc aatgcgtaga cacgaacacg cgagccgttt tgcctgcaaa ccatcgaatc
      181 cggtaagcgt tcagtcgtcg gggcctgggg cagggattcc ttgttgatgc agcgaatctt
      241 gcgggcatgg agcgcgccga ggcggaggcg atctatgact cggggcggga ggcgtgtgtc
      301 gagttcattc tcgatctcgc gggccgggtt gagcgacacg aggagcggct ggcgcgctta
      361 gaggcccagc tgcgtcagga ctcacgttcg agctccaagc cgccatcgtc ggatccgccc
      421 aagacgcgcg ctcagcggcg agcggaggcg cgcgccaagg ccaaggagct catgcgccgg
      481 gagggggagc ggcgtacggc tgggggtcag tctgggcatc ggggtgccgg tcgcgagctg
      541 cggcccgagg atcaggtgga tgagatcgtc gatcactatc ccggtgcgtg cggtggatgc
      601 gggcgtcggt tcgctgccga gcagcgcagg ccgagccgac ggttcggtcg ccatcagatc
      661 gccgagctac cggcgatcag cgtgcgctgg accgagcatc gcacccacca gttgcgctgt
      721 cggcactgcc gcgcccggac gagcgctcgg ttgccggagc ccctcggggc ctgcgcgttc
      781 ggcccgagat tgcaggcggc gatcgtgacg ttgaccggcc gccatcggat ctcccggcgc
      841 ggcatcaccg agctcgcccg cgacctcttc ggcgtcaccc tggcgaccgg cagcgtggac
      901 gcgatctgcc agcgcgcctc ggaggccttg gccggcccgc atctgcaact gcacgactgg
      961 gtgcttgagc agggctcgct gcatgtcgac gagaccggct ggcggaccgc cggtgagggc
     1021 cgcgctttgt gggccgcgac caccccgaag gccgcgttct ttgagatcgc cgagcattgc
     1081 aaccgcgagc agttcaccgc gctgatcggc ccctacccgg ggatcgtggt ctctgaccgc
     1141 tggaacggct atgcccatct cgacccccac cagcgccaag tgtgctggtc gcatctgcag
     1201 cgtgatttcc gtcgccacgc cgatggcctg ggcgagcaga agaccttcgg cgaacagggt
     1261 ttcccgctca ccaagcaggt gttcgccgcc tggcgcgcct accagcacga tcaccacgac
     1321 cgcgaccgac tccaagccga gatcgccccg atccagacgc agctgcgaca gctgctcgag
     1381 gccgcctcgc ccaagagccg gcgcaaccgc tggcaccgcc agttcgccac caacttgctc
     1441 aaggtctggc ccgccctatg gaccttcacc accgtcgatg gcgtcgagcc gaccaacaat
     1501 ccggccgagc gcgcactccg cggcccggtc atccaccgca aactctcgct cggcacccaa
     1561 agccacgccg gcgagcggtt tgccgagcga gtcctctcga ccgccaccac ctgccgcctg
     1621 caaggccgct cgctcttcac ctacctcagc gatctgatca ccgcgcataa ccgcggcgac
     1681 ccgttccccg cgctcgcctg accccgggaa ctgaacgctt acaaaagacc cggatttgcg
     1741 ggccttttgc atgccggagg agggactcga acccccgaca cgcggattat gattccactg
     1801 ctctaaccag ctgagctact ccggcggggg aggaaaagac tagcggctcg ccccgacgca
     1861 aagagtcagc cacgcggcga cgcccgcggg tcgcgacggt cctgttcgtc gatcgtccgc
     1921 ggcgcgccag gggttagcgc tcgcgcggcg ggtcggccgg ggtgtcgccg cgcttctcgg
     1981 tgaagaagat cgtctgcggg agcggctcgg gcggcggctt gggcggcgat ggttggggct
     2041 gtggctgggc ggccattcgt ttaggaggct agcgccgccg cgagagccag tccactgagc
     2101 tcggccagga gaccgagcag tccgacgctg aaggcggtgt gcagccgatc gacgatcggc
     2161 tggttgcctt ctcgccggcg cgtgagcgtg gcgatcatcg tctcgtggta gaggtcctga
     2221 tcctcgagga tctcggcgtc gcggccgcgg atggcggcca gagtggcgct ggcgttgacg
     2281 ctgaacgcca gccgcctggg gtgcagaacg tacgcgccgg ccaggacgag tatgacggcg
     2341 ccgagcagcc cgacgacgg
//
LOCUS       DASVXS010000262        13671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1039300, whole genome shotgun sequence.
ACCESSION   DASVXS010000262 DASVXS010000000
VERSION     DASVXS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039300"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF752_14925"
     CDS             complement(<1..636)
                     /locus_tag="VF752_14925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEX8856886.1"
                     /translation="MIITVTLNAAIDKTLAVPNFRLGRRHRAVEQTSMAGGKGVNVAR
                     ALKALGQPVIATGVAGGPTGTRIIEQLTEEAILNDFVRIREESRTSTAVVDPTSGEQT
                     EINERGPTVAEAELELFVDKLLYLAKGAGVCVFAGSLPRGVDNELYAQLIGEMHKLGV
                     TTVVDAEGDALRHAARAEPTVVSPNELEAEELVGHEFADDDDRRTAVREMIG"
     gene            complement(633..1871)
                     /locus_tag="VF752_14930"
     CDS             complement(633..1871)
                     /locus_tag="VF752_14930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX8856887.1"
                     /translation="MISRSRSRLAALAAACALALLAAATAEAAPPQPRPHLGSDSAIL
                     IDANSGDVLLQQAAAARRPIASTTKLMTARLALERAKPSEVFTAPAYHALPQESQIHL
                     RAGERMAVRDLMRALLLESANDAAETLAVGVSGSVRSFVHSMNAEARKLGLSHTHYTN
                     PIGLDQGPNYSSARDLATLARLLMQERAFARIVDMTQARLTSGSHLRVVHNRNLLVGR
                     YPFVNGVKTGHTRKAGYVLVGSGTSQDAHVISVVLGEPSEAARDNDTVTLLRYGLGQF
                     HRVRVLSAARPVAKLDVKYFGGKAGVGVARNVAVSVRRGRRAQVKVSLPDELEGPLPA
                     GRRVGSARIYTGGRLVKRVALVTKEPVPGASTLRKVATTVGPALITLVVLAVLFAGTL
                     VVLRARAVRTRRRSPRTTTR"
     gene            complement(1868..2197)
                     /locus_tag="VF752_14935"
     CDS             complement(1868..2197)
                     /locus_tag="VF752_14935"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX8856888.1"
                     /translation="MSRRSRSPARLLAPIALIVALIAFVVVVTSSGTSDNSGSKSTSS
                     SATTQRSTTNKKAPAATRRSYTVKVGDTLGAIAERTGVPVATLQELNPALDPQNLVAG
                     QKIKLRE"
     gene            complement(2249..3463)
                     /locus_tag="VF752_14940"
     CDS             complement(2249..3463)
                     /locus_tag="VF752_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856889.1"
                     /translation="MKTSAGALVALAISLTAAAPTSAGIYGTEPIPVSVGPHGEAANG
                     PSGGAAISGDDRYARLVAFHSSASNLTPRDTNGAADVFVWSRPRGRAGLRLARPARPT
                     GRLVRASVSNHGRQANGPSWNPSLDGSMRTRPHCVAFQSNATNLAPGDRDAVSDVYVR
                     DLRSHRTRLVSRGVAAAAVNPAIDGRCARVAFEAGGLVYTASARRGRPHSLGAGSSPS
                     ISRDGSALVWQRGDAVMLRRAGHTSQVVASGHDATVSDSEFAVWGVSFETGSRLTGND
                     GNPGVDVYMRVFGRSGGSRRTDLISAIRRGGSSLGGNSLNGGISVYAANRGIVTFANA
                     NGGGTTLWYRNNHSGNIDDLAHAAGSIFDIATSARANFVAFSSTAANFRFDGNGGVQD
                     VFFKHLVDGRPL"
     gene            3595..4563
                     /locus_tag="VF752_14945"
     CDS             3595..4563
                     /locus_tag="VF752_14945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8856890.1"
                     /translation="MCQAMPSDSRKTVYVALGANAAIAVAKAAGGLVSGSSAMLAEAA
                     HSVADTTNQVFLLVSLSLSERKPDETHPFGYGQERFFWAFLVAVFIFVIGGLFSIGEG
                     VLRLFESESSDTKGFVVSYVVLGVAFVAETVSLIRAWRQTRAEARHAGLPFERFLRQS
                     RNPTSKTVVFEDGAAIAGILLAFAGVGLHQLTGDKTFDAAASILIGLLLIGVAIGLGR
                     DTKGLLLGESARPEERKALYEAIERHDEVEDVLELLTMVLGPESLLVAARLDLAPGLD
                     SDRLEELSSHIDRDLREAVPAVSEVFLDPTPRRELSGDGRGIASRR"
     gene            complement(4759..5715)
                     /gene="pyrF"
                     /locus_tag="VF752_14950"
     CDS             complement(4759..5715)
                     /gene="pyrF"
                     /locus_tag="VF752_14950"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX8856891.1"
                     /translation="MAHRLEDIGEGGGSTAPFADRLCERVEARSSQLVLGLDPDPAAL
                     WFEAAGSGSSASAREVAQLTADAVERHCRLAIEAAGPFCVAVKLQFACFERLGGPGID
                     AAYAVADAAREAGLLVIADAKRGDVPVSAKAYAQAFLGSTPGPFGDVPGFGADALTVN
                     PLLGRDALEPFTAAAAAAGAGLFVLVRTSNPGAADLQDLPAPDRPLHERIAELVASLG
                     AGDDSACGLSMVGAVTGATRPEMLDRLRELMPRAVFLLPGVGAQGGRVEELAAAFRPH
                     PAAGLVTASRSIVSAYKERGGDPARAAAEAAEELRAAAWSLV"
     gene            complement(5712..5993)
                     /locus_tag="VF752_14955"
                     /pseudo
     CDS             complement(5712..5993)
                     /locus_tag="VF752_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(6140..7507)
                     /locus_tag="VF752_14960"
     CDS             complement(6140..7507)
                     /locus_tag="VF752_14960"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8856892.1"
                     /translation="MSGANGELLEDTARRAVEAAVSAGADQAEAWAEEAVTRQIRVYE
                     ESVESLKDAGSRGIGIRVFTGNRSGYAYGTDLSDEGIAELARTAGENARVADEDEFAG
                     LPERPGAAEVSGLYSPELANWNTERKVQLAVEVERTARANPQVSQVEDVVYADAEGRI
                     ALANSAGFVSSFQSSSAWSYVSAFAGEGADLMTGLGVGLARDPSGLDPEAIGREGAER
                     AAALVGARQPESRHCPVVFDAFVAASFIGFIGGMLSADAVQRGRSLFADKEGEEIASG
                     ELRLVDDGTDAEGPDSSPFDGEGSPRRRTPLIEDGRLLTFLFDARTARKAGRETTGNA
                     GRGSYRTPPSVSTSNLILDAGSGGSLEDLARTVGDGLYVTDVAGLHSGVNPVSGTFSV
                     GATGRLIENGELGTPVREMTIASDLVSMLKAVEAVGSTTRWVPFGGSVKAVPLLLREM
                     SVSGS"
     gene            complement(7504..8901)
                     /locus_tag="VF752_14965"
     CDS             complement(7504..8901)
                     /locus_tag="VF752_14965"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8856893.1"
                     /translation="MSLLDPALAARLLDRALARGGDLAEVYVEERNGLALSLDDHRLE
                     RPQSGRELGASVRVVDGDSTYFGHVDGLREDDLIRVADSVAQGVREQRAREGGVALTE
                     PAEPRTHHVIETRPELVDAHVKADILHSCDERARSQGGSEVAQVSVGYAESRRQIEVF
                     NSDGVSARDDRTRVRLSVQVVARRHGTVETGSETRGGHAGFELIEVNPEEVAESALRK
                     AVTLLDAVDAPTGRMPVVVGNGFGGVLLHEAVGHGLESDAIQKRASVYAGRLGEQLTE
                     DFVVAYDNGARENDWGSDGIDDEGTPTQQTTIIENGTLSSYLYDLLRARKDGTKSTGN
                     GRRESFRHLPIPRMTNTYFGPGNASKDDLISGVERGLYAVSFGGGQVEPATGDFVFGV
                     SEGYLIENGKVTAPVRGATLIGNGLEALRAIDGIAADLEIATGYCGKGGQTVPAGVGQ
                     PHVRLRALTVGGTAS"
     gene            complement(8898..9101)
                     /locus_tag="VF752_14970"
     CDS             complement(8898..9101)
                     /locus_tag="VF752_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856894.1"
                     /translation="MKQRKNPLGRAAELAAGAAATVRGRRADRKPRAVIYDAAGHPRV
                     VNADSPEGAALVETAEAMLEVRE"
     gene            complement(9098..9460)
                     /locus_tag="VF752_14975"
     CDS             complement(9098..9460)
                     /locus_tag="VF752_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856895.1"
                     /translation="MADPALTPELALPYLAELSTDIRAAVLLDQAGRTEAHSLASDES
                     AERLRELTLELFERAEKADAEGRAVAEVEVSTGNGSVYAVRSPRWTIAVVSGRFALSS
                     LMLYDLRSVLSDLEPKAA"
     gene            9479..9655
                     /locus_tag="VF752_14980"
     CDS             9479..9655
                     /locus_tag="VF752_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856896.1"
                     /translation="MPLSQISWLVTVGVCLLAALLFLISGYVGYFGILLAIGAAAAVN
                     LLDWPHPRPPEDKD"
     gene            complement(9663..10577)
                     /locus_tag="VF752_14985"
     CDS             complement(9663..10577)
                     /locus_tag="VF752_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8856897.1"
                     /translation="MITGLVAGSPEHTGQKTRAAALSIASNSALIALKVAAGIVTGSI
                     AILTEAIHSSIDLLASVIAFFSVRKAGEPADESHPYGHEKVENVSAALEGVLILVGAA
                     VIVFESIRRLANPPQIDTLGIGIAVIAFSMVANIGVSTYLFRKAQLTQSPALEGDAEH
                     LRTDALTSAGVLLGLVLVQVTGVKAFDAITALAVAGAIVWAGLRIITQSTRVLVDEAL
                     PQEEMEAVHEAIDSYGATEIVGFHKLRARRAGSRRYVDLHVQFRTGTTLERAHEVSHQ
                     LQGRIRDALHGADVLIHLEPEDAARPRR"
     gene            complement(10574..11164)
                     /gene="rdgB"
                     /locus_tag="VF752_14990"
     CDS             complement(10574..11164)
                     /gene="rdgB"
                     /locus_tag="VF752_14990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX8856898.1"
                     /translation="MKLVLATRNAHKLREFEGLLPGHEIVPLPEHVELPPENGDTFEA
                     NAETKARTAANATGLPAIADDSGISAAALGGAPGVRSARFAGENATDEDNLAKLLREV
                     PADADTRVEYVCALAYAEPGDPHTHMFVGRCAGRLTHEPRGEGGFGYDPAVIPSDLDD
                     GRTMAELTPREKDAISHRGRAARKFLDWLERERAAA"
     gene            complement(11182..11919)
                     /gene="rph"
                     /locus_tag="VF752_14995"
     CDS             complement(11182..11919)
                     /gene="rph"
                     /locus_tag="VF752_14995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX8856899.1"
                     /translation="MTRADGRTSPDQIRPTTIQPGFVRSATGSALIECGGTRVICTAT
                     AVEDVPRWMTGAGRGWLTAEYGMLPASTGERKPRDVTKGRADGRTVEIQRLIGRALRG
                     IVDFNALGERTIYVDCDVLEADGGTRCASITGGYVALQLACARLVEEGKLGSIPLTQS
                     IAAISCGIVDGVALLDLNYPEDSSADVDMNVVMTGDDGLVEVQATAERMPLSRASLDE
                     LLALAAGGIQQLREVQAAATGGLPVTA"
     gene            complement(11916..12779)
                     /gene="murI"
                     /locus_tag="VF752_15000"
     CDS             complement(11916..12779)
                     /gene="murI"
                     /locus_tag="VF752_15000"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX8856900.1"
                     /translation="MISVPEIHKELPVAVFDSGVGGLTVLHECLVSLPHEDFVYLGDT
                     QHFPYGDRSVEELQAFAFDLAGQLLDRGAKLLVVACNSATAAVLPTLRAEFGGTVDVL
                     GVVAPESRLAAAATRSGRVGLLATQTTVESGAYERALAEAAPGAELFPVACPELAPLI
                     QRDGEFDEEVVEAVERGCDPLRDAVVDTVILGCTHYPLVRPMIQRALGRGVTIVSSGH
                     AIAEDVERVLRERGLENDESRRGRYRFLCTGEPESFRRVGTRFLQMPLGEVQRLDSQS
                     TTELIAARRVA"
     gene            complement(12803..>13671)
                     /locus_tag="VF752_15005"
     CDS             complement(12803..>13671)
                     /locus_tag="VF752_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8856901.1"
                     /translation="SALAVRFVAELMRPSVPVGLSGQGADEPLAGYGRHFGVRLAGRL
                     RALGPLPRLAAKLAQRTGRTQLRRGLETLEARSDAEMLMTAYRVLTPELKGRLYRPEL
                     KRQLAGSRPEGAVEHYRARVAQLDPLSQILYVDTRLSLPDELLMIADKMSMAESVELR
                     VPLLDEDLVALVESMHPSMKLRGREGKSIHKEAMLRLLPQEIVQRPKVGWETPLDSWL
                     RDELRPLLEDVLLSERGVCRELFEEDALRELIEEHATGARDRTRELFCLLSLGLWHRA
                     YVETPAPAAAAG"
BASE COUNT         1931 a         4940 c         4759 g         2041 t
ORIGIN      
        1 cccgatcatc tcgcgcaccg ccgtgcggcg gtcgtcgtcg tcggcgaact cgtgccccac
       61 gagctcctcc gcctcgagct cgttcgggct caccacggtg ggctctgcgc gcgccgcgtg
      121 gcgcagggcg tcgccctccg cgtcgacgac cgtcgtgacg ccgagcttgt gcatctcgcc
      181 gatcagctgc gcatacagct cgttgtcgac gccgcgcggc aggctcccgg cgaacacaca
      241 cacgcccgcg cccttcgcga ggtagagcag cttgtcgacg aacagctcga gctccgcctc
      301 ggccacggtc ggcccgcgct cgttgatctc ggtctgctcg cccgacgtcg ggtcgacgac
      361 cgccgtcgac gtgcgcgact cctcccggat gcgcacgaag tcgttgagga tcgcctcctc
      421 ggtgagctgt tcgatgatgc gcgtgcccgt cgggcctccg gccacgccgg tcgcgatcac
      481 cggctggccg agcgccttca gcgcgcgggc gacgttcacg cccttgccgc ctgccatcga
      541 ggtctgttcg accgcccggt ggcgcctgcc gagcctgaag ttgggtaccg cgagcgtctt
      601 gtcgatcgcg gcgttgaggg tgacggtgat gatcatcgtg tcgtcgtcct cggactgcgg
      661 cggcgcgtcc tcacggcacg tgcccgcagt acgaccagcg tacctgcgaa caggacggca
      721 agcaccacca gggtgatgag ggccggtccc accgtcgttg ccaccttgcg gagcgtgctc
      781 gccccgggca cgggttcctt ggtcacgagc gccactcgct tcaccagacg gccgccagtg
      841 tagatgcgcg cgctgccaac gcggcgaccg gccggcaagg ggccctcgag ctcgtccggg
      901 agcgacacct tcacctgcgc gcggcggccg cggcgcacgc tgacggcgac gttgcgcgcg
      961 actcccacgc cggcctttcc gccgaagtac ttcacgtcga gcttcgcgac cgggcgggca
     1021 gccgacagca cgcgcacccg gtggaactgg ccgaggccgt agcgcagcag cgtaaccgtg
     1081 tcgttgtcgc gcgcggcctc gctgggctcg ccgagcacga cgctgatcac gtgcgcgtcc
     1141 tgcgacgttc cggagccgac cagcacgtag cccgccttgc gcgtgtgccc ggtcttcacg
     1201 ccgttcacga acgggtaccg cccgaccagc aggttccggt tgtggaccac tcgcaggtgc
     1261 gaaccgctcg tgaggcgcgc ctgcgtcatg tccacgatcc gcgcgaacgc gcgctcctgc
     1321 atcagcagtc gcgcgagcgt cgccaggtcg cgcgcgctcg agtagttcgg accttggtcg
     1381 agcccgatcg ggttcgtgta gtgagtgtgc gagagcccga gcttgcgggc ctctgcgttc
     1441 atcgagtgga cgaagctgcg caccgatccg gagaccccga cggccagcgt ctccgcggcg
     1501 tcgttcgcgc tctcgagcag cagcgcgcgc atcaggtcgc ggacggccat ccgctcgccg
     1561 gcgcgcaggt ggatctgcga ctcctgcggc agcgcgtggt acgcgggcgc ggtgaacacc
     1621 tcgctcggct tggcgcgctc gagcgcgagc cgcgccgtca tcagcttcgt ggtgctcgcg
     1681 atcgggcgcc tcgctgcggc cgcctgctgc agcagcacgt cgccagaatt ggcgtcgatc
     1741 aggatcgccg agtccgagcc gaggtgcgga cgcggctgag gcggcgcagc ctcggccgtc
     1801 gccgccgcca agagcgcaag cgcgcacgcg gcggccaacg ccgcgagccg tgatcttgag
     1861 cggctgatca ctccctcagc ttgatcttct gcccggccac cagattctgg ggatcgagcg
     1921 cggggttcag ctcctgcagc gtcgcaaccg gcacaccggt gcgctctgcg atcgcgccga
     1981 gcgtgtcacc cactttgacg gtgtacgagc gccgagtcgc ggccggcgcc ttcttgtttg
     2041 tggtgctgcg ctgggtggtc gcgctgctgc tcgttgactt cgagcccgag ttgtcgcttg
     2101 tccccgagga ggtcaccacg acgacgaacg cgatcagcgc gacgatcagc gcgatcggcg
     2161 cgagcaggcg tgcggggctg cgggaacggc ggctcatcgc gcgccgatgg tacctccgcg
     2221 cccagcgacc tcgccccagg tgggcgcgtc agagggggcg gccgtccacc aggtgcttga
     2281 agaacacgtc ctgcaccccg ccgttcccgt cgaagcggaa gttcgccgcg gtggacgaga
     2341 atgcgacgaa gttcgcacgc gcgctcgtgg cgatgtcgaa gatcgagccc gccgcgtgcg
     2401 cgaggtcgtc gatgttgccg ctgtgattgt tgcggtacca aagcgtcgtg ccgccgccgt
     2461 tcgcattcgc gaacgtcacg atgccgcggt tcgcggcgta cacggagatg ccgccgttca
     2521 gactgttgcc gccgaggctc gagccgccgc ggcggatcgc cgagatcagg tccgtgcggc
     2581 gcgaaccacc gctgcggccg aacacgcgca tgtagacgtc cacgccgggg ttgccgtcgt
     2641 tgccggtcag ccgcgagccg gtctcgaacg acacgcccca gactgcgaac tcggagtcgg
     2701 agaccgtggc gtcgtggccg ctcgcgacca cctgcgaggt gtgcccggcg cgtcgaagca
     2761 tcaccgcgtc accgcgctgc cagacgagcg cgctgccgtc gcgggatatc gacggcgacg
     2821 agcccgcacc gagcgagtgc ggccgccccc gccgcgccga ggccgtgtag acgaggccgc
     2881 cggcctcgaa cgccacgcgc gcgcagcgcc catcgatcgc cggattcacc gcggcggccg
     2941 cgacgccgcg cgagacgagc ctcgtccggt gcgagcgcag gtcgcgcacg tagacgtcgg
     3001 agaccgcgtc gcgatcgccc ggtgcgaggt tcgtcgcgtt cgactggaac gcgacgcagt
     3061 gcgggcgcgt gcgcatcgag ccgtcgaggg agggattcca cgacgggccg ttcgcctggc
     3121 ggccgtgatt cgacacgctt gctcggacca gccggcctgt gggccgcgcc gggcgtgcga
     3181 ggcgcaagcc agctctgccg cgggggcgcg accagacgaa gacgtcggcc gccccattcg
     3241 tgtcgcgggg ggtgaggttg ctcgccgacg agtggaaggc aacgaggcgc gcgtagcggt
     3301 cgtcgcccga gatcgccgcg ccgcctgagg gcccgttcgc ggcctcgccg tgcgggccga
     3361 ctgaaaccgg gatcggctcg gtgccgtaga tgcccgccga ggtgggggct gcggcagtaa
     3421 gggagatcgc aagcgcaacg agggcgccgg cagacgtctt cactaggttc gccccttctt
     3481 cgtgtgtcag ggcacagtgg atcgtgcccc ggcgggagag tacttccagc tcttcgtcgt
     3541 ctttgcacag gttcaggcgc ttggcgttat gcctgtccca ctgctgggca ccccgtgtgc
     3601 caggccatgc cgtccgacag ccgcaagacg gtgtacgtag cactgggagc gaacgctgcc
     3661 atcgccgtcg cgaaagccgc cggcgggctg gtgagcggct cctccgcgat gctcgccgag
     3721 gcagcccact cggtcgccga cacgacgaat caggtgttcc tgctcgtgtc gctctcgctg
     3781 agcgagcgca agcccgacga gacgcacccg ttcggttacg gccaggagcg cttcttctgg
     3841 gcgttcctcg tcgcggtctt catcttcgtg atcggcgggc tcttctcgat cggcgagggc
     3901 gtgctgaggc tgttcgaaag cgagtcctcc gacacgaagg gcttcgtcgt ctcgtatgtg
     3961 gtgcttggcg tggcgttcgt ggccgagacc gtctcgctca tccgcgcctg gcgtcagacc
     4021 cgcgcggagg cacggcatgc cggattgccg ttcgaacgct tcctgcgcca gagccgcaac
     4081 ccgacgtcga agacagtggt gttcgaggac ggcgcggcaa tcgcaggcat cttgctcgcg
     4141 ttcgcgggcg tggggctgca ccagctcacc ggcgacaaga ccttcgacgc ggccgcttcg
     4201 atactcattg gcctgctgct gatcggggtg gcaatcgggc tcgggcgcga cacgaaggga
     4261 ctcctgctcg gcgagtcagc ccggccagag gagcgaaagg cgctgtatga ggcgatcgag
     4321 cgccacgacg aagtggagga cgtgctcgag ctcctgacga tggtgctcgg gccggagtcg
     4381 ttgctcgtcg cggcgcggct cgacctcgcc cccggactgg actcggatcg cctcgaagag
     4441 ctatcgagcc acatcgaccg cgacctgcgg gaggcggtgc cagcggtgag cgaggtgttc
     4501 ctcgacccga cgccgcgtcg tgagctgagc ggtgacgggc gggggatcgc ttcgcgcagg
     4561 tgatccacgg gtcgccgctg cgcgctgccc taactcctga ctttggttct catatgcgga
     4621 accaaactca gcagttcgct tccgaacgag cgtccgtcgt cactcagggg tgtatatgca
     4681 ccctgagcga cgacacccgc ccgggaccca cgccgttatt gctgctgttc acgcgtcgtc
     4741 ggtcgaaacg gaccgcggtc agacgagcga ccacgccgcc gcgcgcagct cctcggccgc
     4801 ctcggccgca gcccgtgccg gatcgccgcc gcgctccttg tacgccgaca cgatcgagcg
     4861 ggacgccgtc acgagcccgg cagccgggtg cggccggaac gccgccgcga gctcctcgac
     4921 ccttccgccc tgggccccca cgccgggcag caggaagacg gcgcgcggca tcagctcgcg
     4981 caggcgatcg agcatctcgg gccgagttgc ccctgtaacg gcgccgacca tcgacaggcc
     5041 gcacgccgag tcgtcgcccg cgccgagcga agccaccagc tcggcgatcc gctcatggag
     5101 cgggcggtcg ggcgccggca ggtcctggag gtcggcggcc cccgggttcg aggttcggac
     5161 gagcacgaag agcccggcgc cggccgcggc agccgccgcg gtgaagggct cgagcgcgtc
     5221 gcgcccgagc agcggattca cggtgagcgc atcggctccg aagcccggta catccccgaa
     5281 cggcccaggt gtagagccga ggaacgcctg tgcgtacgcc tttgcgctga ccggcacgtc
     5341 cccgcgcttg gcgtcggcga tcacgagcag gcccgcctcg cgcgcggcgt ctgccaccgc
     5401 gtacgccgcg tcgatgccgg gtccgcccag acgctcgaag caggcgaact gcagcttcac
     5461 cgcgacacag aagggacccg cggcctcgat cgcgagccgg caatgccgct cgaccgcgtc
     5521 ggccgtgagc tgcgccacct cacgggccga ggcactcgac ccggatccgg ccgcctcgaa
     5581 ccagagcgcg gcggggtccg ggtcgagccc cagcaccagc tgactgctgc gcgcctcgac
     5641 gcgctcgcag agacgatcgg cgaacggggc ggtcgagccg cccccctccc caatgtcttc
     5701 cagacgatgc gctatgagcc cttgacggcc ttcttgagcg cggaccccgc gctgaaccgc
     5761 ggcacacggc tcgcggcgat ctggatgcgc tcgcccgtct gagggttcac gccctggcgc
     5821 gccccgcggt ccgccacaga gaacttgccg aagccggtga agttcacgtc accgccacgc
     5881 gcaagcgttt cctcgatgac ctgaagagag gcgtcaacag ccttggttgc gtcgcccttc
     5941 gagagacccg actgcgacgc gacctgatct acgaactcag ccttcgtcac tgatcctcct
     6001 tggaggtgtc tggaacgggg ccgaacctaa cagcggtttc ggacgaatcc tgattgcaat
     6061 gcggttttta gcacttccag agccgcaaat tgcggatttc gcgaaggctc cgccgcgctc
     6121 gaatggcgca tcgaaccgct caggaacccg aaacggacat ctcgcgcagc agcagcggaa
     6181 ccgctttgac gctgccgccg aagggcaccc agcgtgtcgt cgagcccacc gcctcgaccg
     6241 ccttcagcat cgacacgagg tcgctcgcaa tcgtcatctc gcgcactgga gtaccgagct
     6301 cgccgttctc gatcaggcgt ccggtggcgc ccaccgagaa cgtcccggac acgggattca
     6361 cgcccgagtg gagtcccgcg acgtcggtca cgtagaggcc gtcgcccacc gtgcgggcga
     6421 ggtcctcgag gctaccgccg gagcctgcgt cgaggatcag gttcgacgtg ctcacgctcg
     6481 gcggcgtccg gtacgagccg cggcccgcgt ttccggtggt ctcgcgaccc gccttgcgcg
     6541 ccgtgcgcgc gtcgaagagg aaggtgagca gccggccgtc ctcgatcagc ggcgtgcgcc
     6601 ggcgcggcga gccctcgccg tcgaagggcg agctgtccgg cccctctgcg tcggtgccgt
     6661 cgtccacgag ccgaagctcc ccggacgcga tctcctcccc ctccttgtcg gcgaagagcg
     6721 agcgcccgcg ctgcaccgcg tcggccgaca gcatcccgcc gatgaagccg atgaagctcg
     6781 cggcaacgaa cgcgtcgaac acgaccgggc agtggcggct ctcaggctgc cgcgccccaa
     6841 ccagcgcggc cgcgcgctct gcgccctcgc ggccgattgc ctcgggatcg aggccggagg
     6901 gatcgcgagc gagacccacg cccagtcccg tcatcaggtc ggcgccctcg cccgcgaaag
     6961 ccgacacgta cgaccaagcc gagctcgact ggaacgacga aacgaatccg gcggagttgg
     7021 cgagcgcgat tcgcccctcg gcgtcggcgt acaccacgtc ctcgacctgc gagacctgcg
     7081 ggttggcccg cgcggtgcgc tcgacctcca ccgctagctg caccttgcgc tccgtgttcc
     7141 agttcgccag ctcgggcgag tagagaccgg agacctccgc cgcgcccggg cgctcaggca
     7201 agccggcgaa ctcgtcctcg tccgccacgc gcgcgttctc cccggcggtg cgcgccagct
     7261 cggcgatccc ctcgtcgctc aggtcggtgc cgtaggcgta gccggagcga ttgcccgtga
     7321 acacgcggat gccgatgccg cggctgcccg cgtccttgag gctctccacg ctctcctcgt
     7381 agacgcggat ctggcgggtg accgcctcct ccgcccacgc ctcggcctgg tccgcgccgg
     7441 cggacacggc cgcctcgaca gcacggcgcg ccgtgtcctc gagcaattcg ccgttcgcgc
     7501 cgctcacgag gcggtgcctc cgacggtgag cgcgcgcagg cgcacgtgcg gctgcccgac
     7561 gccggcaggc actgtctggc cgcccttgcc gcagtagccg gtcgcgatct cgaggtcggc
     7621 ggcgatgccg tcgatcgccc gcagcgcctc cagcccgttt ccgatcagcg tggcgccgcg
     7681 caccggcgcg gtcaccttgc cgttctcgat cagatagccc tcggacacgc cgaacacgaa
     7741 gtcgccggtg gcgggctcga cttggccgcc gccgaaggag accgcgtaga ggccgcgctc
     7801 gacgcccgag atcaggtcgt ccttggacgc gttgccgggg ccgaagtagg tgttggtcat
     7861 gcgcgggatc ggcagatgcc ggaacgactc gcgccgcccg tttccggtcg acttggtgcc
     7921 gtccttgcgc gcccgcagga ggtcgtagag gtacgagctg agtgtgccgt tctcgatgat
     7981 cgtcgtctgc tgggtcggcg tgccctcgtc gtcgatgccg tcgctccccc agtcgttctc
     8041 gcgcgcgccg ttgtcgtagg cgaccacgaa gtcctcggtg agctgctcgc cgagccggcc
     8101 cgcgtagacg ctcgcgcgct tctggatcgc gtccgactcg agcccgtgcc cgaccgcctc
     8161 gtgcaggagc accccgccga agccgttgcc cacgacgacc ggcatccgcc ccgtcggagc
     8221 gtccacggcg tcgagcagcg tgaccgcctt gcggagcgcc gactcggcga cctcctcggg
     8281 attcacctcg atcagctcga atccggcgtg gccgccgcgc gtctcgctgc ccgtctcgac
     8341 ggtcccgtgg cgcctcgcga cgacctgcac ggagagccgc actcgcgtgc ggtcgtcgcg
     8401 cgcggacacg ccgtcggagt tgaagacctc gatctgacgg cgcgactccg cgtaccccac
     8461 gctcacttgc gcgacctcgc tgccgccctg cgagcgggcg cgctcgtcac acgagtgcag
     8521 gatgtcggct ttcacgtgcg cgtccacgag ctcgggccgc gtctcgatca cgtggtgggt
     8581 gcgcggctcg gccggctcgg tgagcgcgac gccgccttcg cgcgcgcgct gctcgcggac
     8641 gccctgggcc accgagtcgg cgacgcgaat caggtcgtcc tcgcgcaggc cgtcgacgtg
     8701 gccgaagtag gtcgagtcgc cgtcgaccac gcgcacggac gcgcccagct cgcgcccgct
     8761 ctggggacgc tcgagccggt ggtcgtcgag cgacagcgcg agtccgttcc gctcctccac
     8821 gtacacctcg gcgaggtcgc cgccgcgcgc gagcgcgcgg tcgagcagcc gtgccgcgag
     8881 cgccgggtcg agcaggctca ctcgcgcacc tcgagcatcg cctcggcggt ctccacgagc
     8941 gcggcgccct cggggctgtc cgcgttgacc acgcgaggat gcccggcggc gtcgtagatc
     9001 accgcgcgcg gcttgcgatc ggcgcggcgc ccgcgcaccg tagcggcggc gccggcggcg
     9061 agctccgcgg cgcgcccgag cggattcttt cgctgcttca tgccgccttc ggctccaagt
     9121 cggagagcac gctccgcagg tcgtacagca tcagggagga gagggcgaag cgcccgctca
     9181 caaccgcgat cgtccagcgc ggcgagcgca ccgcatagac cgagccgttg cccgtggaca
     9241 cctccacctc ggcgaccgcg cgcccctcgg cgtccgcctt ttcggcgcgc tcgaacagct
     9301 cgagcgtgag ctcgcgcagg cgctcggccg actcgtcaga cgcgagcgag tgggcctccg
     9361 tccgaccggc ctgatcgagc agaaccgcag ctctgatgtc ggtggacagc tcggccagat
     9421 agggcagtgc gagctcgggg gtgagtgcag gatccgccaa cgcggtcaga atagcgacgt
     9481 gccgctctcc caaatctcgt ggctcgtgac agtcggcgtc tgcctcctgg cggcgctgct
     9541 gttcctgatc tcgggctacg tcggctactt cggaatcctg ctcgcgatcg gcgcggccgc
     9601 ggccgtgaac ctgctggact ggccgcaccc gcggccgccg gaggacaagg actgatccgc
     9661 gctcagcggc gcgggcgcgc ggcgtcctcg ggctccaggt ggatcagcac gtccgcgccg
     9721 tgcagcgcgt cgcggatcct gccctgcagc tggtggctca cctcgtgcgc gcgctccagc
     9781 gtcgtgccgg tgcggaactg cacgtggagg tccacgtagc ggcggcttcc ggcgcggcgg
     9841 gcgcgcagct tgtggaagcc cacgatctcg gtcgccccat agctgtcgat tgcctcgtgc
     9901 acggcctcca tctcctcttg cggcagcgcc tcgtccacga gcacccgcgt ggactgagtg
     9961 atgatgcgaa gccccgccca gacgatcgcg ccggcgacgg caagggcggt gatcgcgtcg
    10021 aacgctttca cgcccgtgac ctggacgagc acgaggccga gtagcacgcc ggccgatgtg
    10081 agcgcgtcgg tgcggagatg ctcggcgtcg ccctcgagcg ccggcgactg cgtgagctgc
    10141 gccttgcgga agaggtaagt ggagacaccg atgttcgcga ccatcgagaa ggcgatcacg
    10201 gcgatgccga tgccgagcgt gtcgatctgg ggcgggttcg cgagacggcg gatcgactcg
    10261 aacacgatca ccgctgcgcc gacgaggatc agcacgccct cgagcgcggc ggagacgttc
    10321 tccactttct cgtgtccgta gggatgcgac tcgtccgcgg gctcgcccgc cttgcgtacc
    10381 gagaagaagg cgatgaccga ggcgagcagg tcgatcgacg agtggatcgc ctcggtgagg
    10441 atcgcgatcg agccggtgac gatcccggcc gccaccttca gcgcgatcag cgcggagttc
    10501 gacgcgatcg acagcgccgc tgcgcgcgtc ttctggccgg tgtgctcggg cgagcccgcg
    10561 accaagccgg tgatcacgcc gcagccctct cgcgctcgag ccagtcgagg aacttgcggg
    10621 cggcgcggcc gcggtggctg atcgcgtcct tctcccgagg cgtcagctct gccatggtcc
    10681 ggccgtcgtc gaggtcggag ggaatcacgg ccgggtcgta cccgaaaccg ccctcgccgc
    10741 gcggctcgtg ggtgagcctg ccggcgcagc gaccgacgaa catgtgggta tgcgggtcgc
    10801 cgggttcggc gtaggcgagc gcgcagacgt attccacgcg cgtgtcggcg tcggcgggca
    10861 cctcccgcag gagcttggcg aggttgtcct cgtcggtcgc gttctctccg gcgaagcgag
    10921 cggagcgaac accgggagcc ccgccgagcg cggccgctga aatgcccgag tcgtcggcga
    10981 tcgccgggag ccccgtggcg ttcgcggccg tgcgcgcctt cgtctccgcg ttcgcttcga
    11041 acgtgtcgcc gttctcgggc ggcaactcga cgtgctcagg caacggcacg atctcgtggc
    11101 ccggcaggag cccctcgaac tcgcgcaact tgtgcgcgtt gcgcgtggcc agcacgagct
    11161 tcaccgccag cctccccgcg gctacgccgt gacgggcagc ccgccggtcg cagcggcctg
    11221 cacctcgcgc agctgctgga tgccacccgc ggcaagcgca agcagctcgt cgagcgaggc
    11281 ccgcgagagc ggcattcgct cggccgtggc ctgcacctcg acgaggccgt cgtcgccggt
    11341 catgaccacg ttcatgtcga catcggcgct cgagtcctcc gggtagttca ggtcgagcag
    11401 cgcgacgccg tcgacgatcc cgcacgagat cgcggcgatc gactgcgtga gcggaatcga
    11461 cccgagcttc ccctcctcca cgagccgcgc gcacgccagc tgcagcgcca cgtatccgcc
    11521 ggtgatcgac gcgcagcgcg tgccaccgtc ggcctcgagc acgtcgcagt ccacgtagat
    11581 cgtgcgctcg ccgagcgcat tgaagtcaac gatgccgcgg agcgcccgtc cgatcaggcg
    11641 ctgaatctcg acggtgcgtc cgtcggcgcg gcccttggtg acatcgcgcg gcttgcgctc
    11701 gcccgtggac gcgggcagca tgccgtactc ggcggtgagc cagccgcgcc cggccccggt
    11761 catccagcgc gggacgtcct cgacagccgt cgccgtgcat atcacgcgcg tgccgccgca
    11821 ctcgatcagc gccgagccgg tcgcggaccg gacgaacccg ggctggatcg tggttggccg
    11881 gatctgatcc ggcgaggtgc ggccgtcggc gcgcgtcacg ccaccctcct cgccgcgatc
    11941 agctcggtcg tggactgcga gtcgagccgc tgcacttcac cgagcggcat ctgcaggaag
    12001 cgggtgccca cgcgcctgaa cgattccggc tcacccgtgc agaggaagcg gtaccggccg
    12061 cggcggctct cgtcgttctc gagaccgcgc tcgcgcagca cccgctcgac gtcctcggcg
    12121 atggcgtgcc ccgacgagac gatcgtcacg ccccggccca gcgcgcgctg gatcatcggg
    12181 cggacgagcg ggtagtgggt gcagccgagg atcaccgtgt ccacgacggc gtcgcgcagc
    12241 gggtcgcagc cgcgctcgac cgcctcgaca acctcctcgt cgaactcgcc gtcgcgctgg
    12301 atcaggggcg cgagctccgg acacgcgacc gggaacagct cggccccggg cgcggcctcg
    12361 gcgagcgccc gctcgtaggc gccgctctcg acagtcgttt gcgtggcgag caagccgacg
    12421 cggccgctgc gcgtggccgc cgcggccagc cgtgactccg gcgcgacgac gccgaggaca
    12481 tcgaccgtgc cgccgaactc cgcgcgcagc gtggggagca cggcggcggt ggcggagttg
    12541 cacgccacca caagcagctt cgcgccgcgg tcgagcagct gaccggcgag gtcgaacgcg
    12601 aacgcctgaa gctcctccac cgagcggtcg ccgtagggga aatgctgggt gtcgccgagg
    12661 tacacgaaat cctcgtgggg cagcgacacg aggcactcgt gcagcactgt gagcccgccg
    12721 acgcccgagt cgaagacggc gacgggcagt tccttgtgga tctccggcac cgaaatcatg
    12781 ttcgcaggaa cctcgacggc tcctacccgg cggcggccgc cggcgcaggg gtctcgacgt
    12841 atgcgcggtg ccacagaccg aggctgagca ggcagaagag ctcgcgcgtg cgatcccgcg
    12901 cgccggtcgc gtgctcctcg atcagctcgc gcagcgcgtc ctcctcgaac agctcgcggc
    12961 atacgccacg ttccgacagc aggacgtcct cgagcagcgg ccgcagctcg tcgcgcagcc
    13021 agctgtcgag cggcgtctcc cagccgacct tgggtcgctg cacgatctcc tgcgggagca
    13081 gccggagcat cgcctccttg tggatcgact tcccctcgcg cccgcgcagc ttcatcgatg
    13141 ggtgcatcga ctcgacgagc gcaaccaggt cctcatcgag taagggcacg cgcagctcga
    13201 ccgactcggc catcgacatc ttgtcggcga tcatcaggag ctcgtcggga agggagagcc
    13261 gcgtgtccac gtacaggatc tgcgagagcg ggtcgagctg tgcgacccgc gcgcgatagt
    13321 gctcgaccgc accctcgggc cgcgagcctg ccagctgccg cttcagctcg ggccggtaga
    13381 ggcgcccctt cagctccggc gtgagcacgc ggtacgcggt catgagcatc tccgcgtcgc
    13441 tgcgcgcctc gagcgtctcg agcccgcggc gaagctgggt gcgccccgtt cgctgcgcga
    13501 gcttcgcggc aagccgcggc agcggtccga gcgcgcgcag gcgcccggcg aggcgaacgc
    13561 cgaagtgacg tccgtagccc gcgagcggct cgtcggctcc ctgtccgctc agccccaccg
    13621 gcaccgacgg ccgcatcagc tcggcgacga agcgcacggc cagcgcgctg g
//
LOCUS       DASVXS010000263         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_755310, whole genome shotgun sequence.
ACCESSION   DASVXS010000263 DASVXS010000000
VERSION     DASVXS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755310"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VF752_15010"
     CDS             complement(<1..324)
                     /locus_tag="VF752_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856902.1"
                     /translation="MPAAVDLLSPKRIAVVASNPTTSPVTRLPIGFWWSELTHPYWEF
                     AQHGYDVAVYSPDGGKLEGDSWSDPRDESGYSAEDLVSLGFISSPDHVGLLQDTKPIA
                     EIDIDD"
     gene            complement(308..637)
                     /locus_tag="VF752_15015"
     CDS             complement(308..637)
                     /locus_tag="VF752_15015"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX8856903.1"
                     /translation="MIVEYIRYRIPEDQGDAFEDAYRRAGASLEASPHCERYEIAHCS
                     DDPRAYTVRIEWDSAEGHMTGFRNSPEFRTFFAAVQPFVGQIEEMRHYDVVASGSEEA
                     SAHARGS"
     gene            complement(634..1467)
                     /locus_tag="VF752_15020"
     CDS             complement(634..1467)
                     /locus_tag="VF752_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8856904.1"
                     /translation="MTPTVHTYRSGEPGLFVNSYLVEGEEGVVAVDAPLLISDGRAYR
                     ARYEALHKPLLAVLVTHPHPDHYNTITELLSGQDVPVIALPRVASEIREKDDAKRAQW
                     SPMFGHEWPSSATFPNRHVDDEQSVEFGDLCFTAWDFGPAESECETVWLLGDGQVAFA
                     GDLAFNGTHAYLADGHSDAWLRALDRAEDALGTVRKLYVGHGEPTTPAVLADQRRYLL
                     MVREVVSRVAGDRPQLTEDEAAEVARLMERYLPDAPLGWLVQAGASAVAAELSQEGAT
                     A"
     gene            1563..>1837
                     /locus_tag="VF752_15025"
     CDS             1563..>1837
                     /locus_tag="VF752_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8856905.1"
                     /translation="MPELRHLRYFVAVADELNFSRAAVRLHMAQPPLSAAIRQLEREL
                     GVELFTRTSREVRLTDAGRAFLDGARRTLAEADRAVDDAKRTGAGELG"
BASE COUNT          277 a          621 c          638 g          301 t
ORIGIN      
        1 atcgtcgatg tcgatctcgg cgatcggctt cgtgtcctgg agcaaaccca cgtggtcggg
       61 cgaagagatg aagccgaggc tcaccaggtc ctccgccgag tagccgctct cgtcgcgcgg
      121 gtcggaccac gagtcgccct cgagcttgcc cccgtccggg ctgtagacgg cgacgtcata
      181 gccgtgctgc gcgaactccc agtagggatg agtcagctcg gaccaccaga agccgatcgg
      241 aagcctggtg accggcgacg tcgtggggtt cgacgccacg accgcgatcc gcttgggtga
      301 gaggaggtca actgccgcgg gcatgtgcac tcgcttcctc tgagcctgag gcgacgacgt
      361 cgtagtggcg catctcctcg atctggccga cgaagggctg cacggccgcg aagaaggtgc
      421 ggaactccgg gctgttgcgg aagcccgtca tgtggccctc ggccgagtcc cactcgatgc
      481 gcaccgtgta ggccctcgga tcgtcgctgc agtgagcgat ctcgtagcgc tcgcagtggg
      541 gcgacgcctc gagcgacgct ccggcgcggc ggtaggcgtc ctcgaaggcg tcgccctggt
      601 cctcggggat tcggtagcgg atgtactcga cgatcatgct gttgctcctt cttgtgacag
      661 ttccgcggcc accgcactcg ctcccgcctg cacgagccag ccgagcggag cgtcagggag
      721 gtaccgctcc ataagccgtg caacctcggc ggcctcgtct tcggtgagct gcggccgatc
      781 gccggcaacg cggctcacta cctcgcgcac catcagcagg tagcggcgct gatcggcgag
      841 aacggccggg gtggtgggct cgccgtggcc cacgtagagc tttcggaccg tgccaagcgc
      901 gtcctcggcc cggtcgagcg cgcgcagcca tgcgtcgctg tgcccgtcgg cgaggtacgc
      961 gtgcgtgccg ttgaaagcga ggtcgcctgc gaatgcgacc tggccgtcgc caagcagcca
     1021 gaccgtctcg cactcgctct cggcgggccc gaagtcccac gccgtgaagc aaaggtcgcc
     1081 gaactcgacg gactgctcgt cgtccacgtg ccgattcggg aaggtcgcac tcgatggcca
     1141 ctcgtgcccg aacatcgggg accactgcgc gcgcttggcg tcgtccttct cccggatctc
     1201 gctcgcgacc cgtggtagcg cgatcacggg cacgtcctgg cccgagagca actcggtgat
     1261 cgtgttgtag tgatccgggt gcggatgcgt gacgagcacg gccagcagcg gtttgtgcag
     1321 cgcctcgtag cgcgcccggt acgcgcgccc gtccgagatc agcagcgggg cgtcgaccgc
     1381 gaccacgccc tcctctcctt ccaccaggta tgagttgacg aatagcccgg gctcgccgga
     1441 gcggtaggtg tgcacggttg gcgtcatgcc ggcgacgcta cgcccacccc actacgcgaa
     1501 caacagcgat ccggtctggc accagatgca gccggtatgg ccgggttagg cttcgctttg
     1561 gcatgccgga gctgcgccac ctgcgctact tcgtggccgt tgcggatgag ctcaacttca
     1621 gccgcgctgc cgtgcggctg cacatggccc agccgccgct gagcgccgcg atccgccagc
     1681 tcgagcgcga gctcggcgtc gagctgttca cgcgcacgag cagggaggtg aggctgaccg
     1741 acgcggggcg cgcgttcctc gacggtgcgc ggcgcacgct cgctgaggcc gatcgcgcgg
     1801 tggacgatgc caagcgaacc ggggcgggcg aactcgg
//
LOCUS       DASVXS010000264         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_755335, whole genome shotgun sequence.
ACCESSION   DASVXS010000264 DASVXS010000000
VERSION     DASVXS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755335"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VF752_15030"
     CDS             complement(<1..426)
                     /locus_tag="VF752_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8856906.1"
                     /translation="MTATNTGATNIFDPPTVNIQAGDSVTWTWGGSNPHSVTSDMSSP
                     MSFDSGIRSPGASFTIQFPTAGAFTYHCIVHPGMNGTVVVAPASGGGGGGDGSPPGAT
                     PGDTVAPAVSALKVQSRWIRFALSEPAKLTGSVKKGARRV"
     gene            559..1638
                     /locus_tag="VF752_15035"
     CDS             559..1638
                     /locus_tag="VF752_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX8856907.1"
                     /translation="MASLDIVDTPEQAQAQELAPLLVREPLEAYLDENELGSGPISAE
                     RIGEGHSNITYLIDRGDERFVLRRPPRPPLPPSAHDVLREARLLKAVEGRIRTPAVLA
                     ACDDESILGVPFYVMKYMTGTVITHAVPQALDNPEERRGIGHELIAALAEVHAVDWQE
                     AGLESFGKPTGYLDRQLRRFNGLWEHNKTRELPVVQEVAEWLEAHKPESGEATIVHGD
                     YRLGNTMFGNDAPAHLVAIFDWELATIGDPLADLGYMTATWTQKDDLTGTMFDNLSVT
                     HGEGFPTRDQLVERYEEITGRSMSDLRWYQALALWKAAVFMEGNFKRSLAGTTDDPYL
                     KLFDVGVPQLAGAALAITRRPAPGS"
     gene            1635..2243
                     /locus_tag="VF752_15040"
     CDS             1635..2243
                     /locus_tag="VF752_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8856908.1"
                     /translation="MKGLILDFGGVLTTNVFESFRAFCEAEGLDPQTVKRTFRENPAA
                     LAELRALERGDVEVPEFERRFAPHLGLEDRAEGLVQRLFAGVSPDEPMVQAVRRARDA
                     GIRTGLISNSWGGTTYDRVAIDDLFDGVVISGEVGLHKPEPEIFELGAERIGLPASEC
                     VFVDDLRENCAGAEAVGMTAILHRGADTTLPELERVLGVDLR"
     gene            complement(2240..>3501)
                     /locus_tag="VF752_15045"
     CDS             complement(2240..>3501)
                     /locus_tag="VF752_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8856909.1"
                     /translation="SDQHPWFQESRASRDNPKRDWYVWRDGRDGGPPNNWPSTFKTVG
                     PAWTFDEQTGQWYLHSFLPQQPDLNWENDEVREAMHGVMRFWLDRDADGFRIDVVHKL
                     AKDPRLSDAVPGRPVPWEDWQPTIHERLRDLRDVVDEYPEKMLVGEVYLFDVERIVAY
                     VNSGDQLHLAHNFVFLHLPWDEAAFRAAIDGFEALSTEAAWPAWFLANHDHPRVASRF
                     NADGNGHLRARAVLMMLYALRGTPFIYQGEELGLPDAEIPRDRIVDVDGRDPERAPIP
                     WSPPSEAGPGAGFTTGEPWLPITSAAEELCVARQDTYLDSTLSLARRIAALRRERPEI
                     HLGEQRSFDAGPDLLAWMCVESTTCRLGIVNFAAEERRFTRSRDVPEQGRLDVSTIPA
                     LDAELGGQIVDLTDFRLAPCEAVLLSI"
BASE COUNT          540 a         1219 c         1215 g          527 t
ORIGIN      
        1 aactcgccgc gcacccttct tcaccgatcc ggtcagcttg gccggctctg acagcgcgaa
       61 ccggatccac cgcgactgca ccttcaaagc gctcactgcc ggcgcgacgg tgtcgcctgg
      121 ggtggcgccg ggaggcgagc cgtcgccacc gccgccaccg cctgaggcgg gcgccacgac
      181 gacggttccg ttcatcccgg gatggacgat gcagtggtac gtgaacgccc cagcggtcgg
      241 gaactgaatc gtgaacgagg cgcccggcga tcggatgccc gaatcgaagc tcatcggact
      301 actcatatcc gaggtcaccg agtgcgggtt gctgccgccc cacgtccacg tgacgctgtc
      361 gccggcctga atgttcacgg tgggcggatc gaagatgttt gtcgcgcccg tgttggtagc
      421 cgtcacatcg gccgccatag ccggcgctgc gagcgccccg acccaggcca ctgtcgccgc
      481 gaggagccgc atatcccgta cgacagggcg gctcgggcga tccttccctg gtttggcgca
      541 tttaggatgc cgcactcgat ggcctccctg gacatcgtcg acactcccga gcaggcgcag
      601 gcgcaggagc tcgcgccgct gctcgtgcgc gagccgctcg aggcgtacct cgacgagaac
      661 gaactgggct cgggaccgat cagcgcggag cggatcggcg agggccactc caacatcacg
      721 tacctgatcg atcgcggcga cgagcgcttc gtgctgcggc gcccgccgcg tccaccgctg
      781 ccgccctcgg cgcacgacgt gctccgcgag gcgcgcctcc tgaaagcggt tgagggcagg
      841 atccgcacgc cggcggtgct cgcggcctgc gacgacgagt cgatcctcgg ggtgccgttc
      901 tacgtgatga agtacatgac cggcacggtc atcacgcacg cggtgcctca ggcgctcgac
      961 aaccctgagg agcggcgcgg gatcggccac gagctgatcg ctgctctcgc cgaggtgcac
     1021 gccgttgact ggcaggaggc ggggctcgag agcttcggca aaccgaccgg ctacctcgac
     1081 cgccagctcc gccgcttcaa cgggctgtgg gagcacaaca agacgcgcga gctgccggtg
     1141 gtgcaggaag tggccgagtg gctcgaggcg cacaagcccg agtccggcga ggcgacgatc
     1201 gtgcacggcg actaccggct cggcaacacg atgtttggca acgacgcgcc ggcgcacctc
     1261 gttgcgatct tcgactggga gctcgccacg atcggcgatc cgctcgccga cctcggctac
     1321 atgacggcga cctggaccca gaaggacgac ctgaccggca cgatgttcga caacctctcg
     1381 gtcacgcacg gcgaaggctt ccccacgcgc gaccagctcg tcgagcgcta cgaggagatc
     1441 accggccgct cgatgtccga tctgcgctgg taccaggcgc tcgcgctctg gaaggccgcc
     1501 gtcttcatgg agggcaactt caagcgctcg ctcgccggca cgacggacga cccctacctg
     1561 aagctcttcg acgtgggcgt gccgcagctg gccggcgccg cgctggcgat caccaggcga
     1621 ccggcacccg gctcgtgaag ggcctgatcc tcgacttcgg cggcgttctc accacgaacg
     1681 tcttcgagtc gttccgcgcg ttctgcgagg cggaggggct cgacccgcag accgtgaagc
     1741 gcacgttccg cgagaacccg gcggcgctcg ccgagctgcg cgcgctcgaa cgcggcgatg
     1801 tcgaggtgcc cgagttcgag cgccgcttcg cgccgcacct gggtctcgag gatcgcgccg
     1861 agggactggt gcagcggctg ttcgcgggcg tgagcccgga cgagccgatg gttcaggccg
     1921 tgcgtcgcgc ccgcgacgcg gggatccgga ccggcctcat ctcgaactcc tggggtggga
     1981 cgacctacga ccgggtcgct atcgacgacc tgttcgacgg cgtggtgatc tcgggcgaag
     2041 tcggtctgca caagcccgag cccgagatct tcgagctcgg ggccgagcgg atcggcctgc
     2101 cggcatccga gtgcgtgttc gtcgacgatc tgcgggagaa ctgcgcgggc gcggaggccg
     2161 tcgggatgac cgcgatcctc caccgcggtg ccgacaccac gctgccggag ctcgagcgcg
     2221 tgctcggagt cgatctgcgc tagatgctca gcagcaccgc ctcgcacggg gcgaggcgga
     2281 agtcggtcag gtcgacgatc tggccgccca gctcggcgtc aagtgcggga atggtcgaca
     2341 cgtcgagccg cccctgctcc ggcacgtcgc gcgaccgtgt gaacctccgc tcttcggcgg
     2401 cgaagttgac gatgccgagc cggcacgtcg tcgactccac gcacatccac gcgagcaagt
     2461 ccggcccggc gtcgaacgag cgctgctcgc cgagatggat ctccgggcgc tcgcgccgaa
     2521 gagcggcgat gcgtcttgcg agcgacagcg tcgagtccag gtacgtgtcc tgccgcgcta
     2581 cgcacagctc ctcggccgcg gacgtgatcg gcagccacgg ctcgccggtc gtgaagcccg
     2641 cgcccgggcc ggcctcggac ggcggcgacc acgggatcgg cgcgcgctca gggtcccggc
     2701 cgtccacgtc gacgatgcgg tcgcgcggga tctcggcgtc gggcagcccg agctcctccc
     2761 cctggtagat gaacggcgtg ccgcgcagcg cgtacagcat catcagcaca gcgcgtgcgc
     2821 gcaggtgacc gttcccgtcc gcgttgaacc gcgacgcgac gcgcgggtgg tcgtggttcg
     2881 ctaggaacca ggcgggccac gccgcctcgg tggagagtgc ctcgaagccg tcgatcgccg
     2941 cgcggaacgc cgcttcgtcc cagggcagat gcaggaacac gaagttgtgg gcgaggtgca
     3001 gctggtcgcc gctgttcacg taggcgacga tccgctccac gtcgaacagg tagacctctc
     3061 cgacgagcat cttctccggg tactcgtcga cgacgtcgcg caggtcgcgc aaccgctcat
     3121 ggatcgtcgg ctgccagtcc tcccagggaa ccggccggcc cgggaccgcg tccgacaggc
     3181 gcgggtcctt cgcgagcttg tgcaccacgt cgatgcggaa tccatccgcg tcgcggtcga
     3241 gccagaagcg catcacgccg tgcatcgcct cgcgcacctc gtcgttctcc cagttgagat
     3301 cgggctgctg tggcaggaac gagtgcaggt accactggcc ggtctgctcg tcgaaggtcc
     3361 acgccgggcc gaccgtcttg aaggtggacg gccagttgtt aggcggtccg ccgtcgcggc
     3421 cgtcgcgcca gacgtaccag tcgcgcttcg ggttgtcgcg cgaggcccgc gactcctgga
     3481 accacggatg ctgatcggac g
//
LOCUS       DASVXS010000265        15886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_254437, whole genome shotgun sequence.
ACCESSION   DASVXS010000265 DASVXS010000000
VERSION     DASVXS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254437"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..1329
                     /locus_tag="VF752_15050"
     CDS             565..1329
                     /locus_tag="VF752_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8856910.1"
                     /translation="MRFAKRLAPALALLLLLACGCGQAGLYSERDGPAKPKGAVLVIH
                     GGAWHVVGPRAVYSMRGYTVRLNRLGYSTLNIDYRRGRQSLPDVLAAYDGLRRELGPG
                     VPICALGTSAGGNLALLLAERRSDVACVVAEAPPTLIAGLTGRVRGYAREYFPTHADM
                     AEYSPALHVDDLHAPVLVAQALHDPTVPYSQSSALVAADPRIRRIALRPGRTPWIHAK
                     VYGPDVPRLDRAEAEFIGAAIVGYRGLLASGPGGGT"
     gene            1367..1975
                     /locus_tag="VF752_15055"
     CDS             1367..1975
                     /locus_tag="VF752_15055"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX8856911.1"
                     /translation="MAYEVPDLPYDYAALEPHIDEQTMRLHHDKHHQTYVDKANAALE
                     GTEWADKPVEEVLKNLDQLPSDKQTPVRNNAGGHYNHSLFWQIMSPDGGGQPDGALAE
                     AIDSAFGSFDDFKTKVNDTGANRFGSGWAWLVWDGSGLAVVDTPNQDSPISNGQVPLL
                     GVDVWEHAYYLKYQNRRPEYLEAWWNVVNWPEVASRFEAARG"
     gene            complement(2017..2622)
                     /locus_tag="VF752_15060"
     CDS             complement(2017..2622)
                     /locus_tag="VF752_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8856912.1"
                     /translation="MATRVRNQRGEGERLRTALLDAASELLAETHDVDQLSVRAVTAR
                     AGVSPTAMYLHFSGKAELTEAVKARSFEALTSVLREADAEHEGDPRAQLIAMGHAYLR
                     FAREQPGQYAILFQTPGGPDKRREDLTQGLGIGGNAFGVLVNAVQRLLGDDVDPFELA
                     CVHWMSLHGLATAKAAMPKFPFPDEHRYIELVVDRIAGARR"
     gene            2738..4810
                     /locus_tag="VF752_15065"
     CDS             2738..4810
                     /locus_tag="VF752_15065"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8856913.1"
                     /translation="MFTSLWRRAVRRPRRVAALAFVAFLLAAVIGGPATGSFEAPHAF
                     DDPGSQGTQAREQIERATGDSSSDVLALVHAPPGSPAVARTASVLRADPDVTRVTVPS
                     PRAPSPLVSRDRGETLIAANVRASARDEDLVKRLEKSLRGDRSVSLGGDAVANTQAGE
                     QATSDLGRAELFAFPLLALLSLLVFRGVAAALPLAVGSLSVLSTFALLRGVNEAVALS
                     PFALNLVIGLGLGLAVDYSLLFVSRFREELGHGSDVSQAVRRTLATAGRTVVFSAVTV
                     AAAAASLTVFPQRFLISMGIGGVIVALVAAAATVLVLPGLLVLLGARIGKVQLGPDRS
                     GRWYGLAQRTMRRPALVATAAAAMLIALAAPAVSLNWTGVDASILPTSKSARVVQDSV
                     TRDFPQADASPVNVVLNAPTSDGASVRAYSAQLKGVQGVRSVSTPRPLDARTWEIDLH
                     TGSDAASGNSQRVVSDVRALRAPAPVLVGGTAADLHDQRAAVSHAVPIALGLLAVLTI
                     AALWLMTGSLVLPAIALVMNLLTVAAATGILVFVFQQGHLAGLIGTSAQPGIEQTDYL
                     VLLAIVFGLSTDYGVFLLSRIKEARDRGLSDQEAIAVGVQRTGAIVSAAAILLTVALG
                     AFVTGKVVFLKELGLGAAVAVLLDAFVVRTLLVPSLLRILGPAAWWSPGPLRRLHGRI
                     GVAEPASA"
     gene            4857..5135
                     /locus_tag="VF752_15070"
     CDS             4857..5135
                     /locus_tag="VF752_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856914.1"
                     /translation="MQAAPKMLVVFALATLGVVGIVIALATGQWWVLAPALLAHLIAS
                     AVAVGYIGARLGQEDKPDPITEARLRDEPRADARTSSPDDRDEPHMAI"
     gene            5170..5499
                     /locus_tag="VF752_15075"
     CDS             5170..5499
                     /locus_tag="VF752_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8856915.1"
                     /translation="MRAFDVKTSEQDGAVRLELSGELDISSAARAEAELRRAEEAKPE
                     LIVLDLRRLAFMDSTGLRLVVGADLRAREEGRRFAIVQGPDAIRRVFEITRLAERLQI
                     VADPSEL"
     gene            complement(5496..5900)
                     /locus_tag="VF752_15080"
     CDS             complement(5496..5900)
                     /locus_tag="VF752_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8856916.1"
                     /translation="MITDLKIRLDNGPQAVSAARLALDALEPHVSSAQLDDLRLLVSE
                     LVTNSVRHAAGGSDDEVRLEVAVGEDVLRAEVVDSGPGFEATPRGTEDDIGSGWGLYL
                     VETLADRWGVETDHVTCVWFELDRTNGSRVAA"
     gene            complement(6142..7212)
                     /gene="murG"
                     /locus_tag="VF752_15085"
     CDS             complement(6142..7212)
                     /gene="murG"
                     /locus_tag="VF752_15085"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX8856917.1"
                     /translation="MARVTIAAGGTAGHVVPALAIADALRDRGADVTFIGGERAEAEL
                     VPKAGYEFARLPVAGIDRRNPVRAARAVALAGLATLKALRLLRRRRPVAVIGGGGYVA
                     GPVGLAAVMLRIPLYLTEADSHLGMANRLLARFAERVFLSFPMKRRRPPRYELSGRPV
                     PRGTTEGDAAAAHERFGIRPDRPCLLVFGGSLGARTLNEAAISAFGRAAPCTVLHACG
                     RRDYASLRERLDALGSPEHYRLEPYIQPFADALAVADLAAARAGGSVLELAAAGVPAI
                     LIPYPHATANHQTGNARYMEREGAAVVIPDSELDGPRLAREVAVLLGSSNRLGAMSAA
                     ARRVARPDAAERIADEVLANPR"
     gene            complement(7130..8413)
                     /gene="ftsW"
                     /locus_tag="VF752_15090"
     CDS             complement(7130..8413)
                     /gene="ftsW"
                     /locus_tag="VF752_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX8856918.1"
                     /translation="MAATRKTERRPRSRAAARPKPPLEYSILYTATLCLLAAGAVMVY
                     SASSAESLLTGPGDPAYYLKRYLILAVIGVAVLHALSRHGLDVMRKATPLLLGASFLL
                     TLAVMVPGVGVNVNGATRWLGAGPLQFQPSELLKVSLVLYAAQVLTTQPRRMRSLKEL
                     ANPLLLVVGAACLLLLKQPDMGTALVVCFSIGALLISAGVPVRHLLTILGVLVFFATI
                     FAIVEPYRRARLMSFVNPWHDATGAGFQSVQSMIAIGSGGMFGRGLGESVQKIFYLPE
                     AHTDMILAIIGEELGLIGISAVAALYGLIAYAGLRAAKLAKDRYSKLVAAGLTSLVLS
                     QATLNFMAVLGLAPLTGVPLPFISYGNSNLIVMLAGMGVLLNVARTGGSGRATSRPRP
                     LRAIDGGARGSGDDRGGRNGRPRRAGAGDRRRATG"
     gene            complement(8458..9771)
                     /gene="murD"
                     /locus_tag="VF752_15095"
     CDS             complement(8458..9771)
                     /gene="murD"
                     /locus_tag="VF752_15095"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX8856919.1"
                     /translation="MGGRPPLPEGPYLVVGLARSGAAAARMLLEHGDGDVRGADSGRP
                     DEAAGLEGVHIALESDGLELLEGVRCLVKSPGVPSDAPAIAAARERGIAVVGELELAW
                     RLLPNEFIAVTGTNGKTTTTELLGAIHREAGVPVAVAGNVGTPLSTLVGELAEDTVVV
                     CEASSFQLEDTKRFAPECAVLLNVTEDHLDRHRTMEAYLAAKLQAFAHQSDRDVAVVP
                     RGFGRPGTAELVEFGGEGADLAQRGGWLEWRRRRLMRAADVRIRGRHNLENAMAAAAA
                     ALAREIEPQAVSRALQSFAGVPHRLEEVAELNGVLFVNDSKATNVDAARVGIEAFEGG
                     IHLILGGSLKGGGFKELRKPVRRRSRAVYVIGEAAERLAADLDGTAELHRSGDLGTAV
                     SQAAAAAQPGEVVLLSPACASYDQYRNFEERGDHFRALVTALAPV"
     gene            complement(9771..10715)
                     /gene="mraY"
                     /locus_tag="VF752_15100"
     CDS             complement(9771..10715)
                     /gene="mraY"
                     /locus_tag="VF752_15100"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX8856920.1"
                     /translation="MASLLICMFLGPKFIDVLKTHEFGQHIREEGPAAHSTKAGTPTM
                     GGLLIVFSVVVPFLILGEYDWETLAVVGTMLASAGLGFVDDWMKISKRRSLGVSGRTK
                     LVVQLLIAIGLWLAASKGVGLSDTLRFRIFDFTANIDGLYPVLIFLVLAGATNGVNLT
                     DGLDGLAAGCAAIVFLAYTAMTFITTGQDDLALVCSCLVGACVGFLWFNSFPASVFMG
                     DTGSLALGGAIAALAVMTKTEVLLLITGGIFVIEALSVLIQVISFQRFRRRVFLMAPV
                     HHHFELLAWSETKIILRFWIVAAIGAAIGFTLYQQSIS"
     gene            complement(10757..12085)
                     /gene="murF"
                     /locus_tag="VF752_15105"
     CDS             complement(10757..12085)
                     /gene="murF"
                     /locus_tag="VF752_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX8856921.1"
                     /translation="MIDRDAAWVARAAGGVLVAGDPDRPGPRRALIDSREVGPGDLFV
                     GLPGSNADGGEFARAALEQGAWGVLVEQSRADQMTRARVGGAVVIGAENPLAALASLA
                     REWRLQLDCPAVGVTGSTGKTSTKDILTALLRPHKRVHSNRENFNTEIGLPLSLLQAE
                     RGTDVLVLEMAMRGEGQIAELVSIAEPQVGVIVNVGPVHLELLGTIERVAAAKAELIR
                     DLPNGGACVVPADEPLLEPHLRGELETITFGEGGDVSLESFSEGRALIDARGERIELE
                     LPFEEPHNLENTLAAVGACLALGVTPRGSVDVQFSSLRGEVLELGGGVTVVNDCYNAN
                     PMSMRAALDHLAATPASRRIAILGGMAELGPEADRWHRELGEHADRLGIDVLITVGEL
                     GLGFAQGYAGETHSVATPEEAGALLEEIAQPGDRVLVKGSRSVGLERVLA"
     gene            complement(12082..13500)
                     /locus_tag="VF752_15110"
     CDS             complement(12082..13500)
                     /locus_tag="VF752_15110"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX8856922.1"
                     /translation="MDLRELMGQGPPDLEITALAYSSKSVAPGALFFCVRGFTSDGHD
                     FAPDAVERGAVALVAERPLDLGVPEVIVPDARAAMAPAAVKFCGDPTAKLQVVGITGT
                     NGKTTTAFLIRDLLEADGRQSGLLGTVKSVVGGQEEPVERTTPEAIDLQHTFRRMLDA
                     GDRAAVMEVSSHALALGRAEGIRFAARVFTNLTQDHLDFHESMEDYYLAKRRLFDDEG
                     PSIVNVDDEYGRRLVSDIGDAITYGIDNDAAYRARDVRFDPSGSRFVIEAPDGAVEIE
                     TRLPGLFNVSNALAAFAAVRQLGVPADTARAGLERAGRVPGRFEPVDEGQDFTVLVDY
                     AHTPEALDNVLRAAREITRGRLHVVFGAGGDRDRGKRPKMGRVASALADRVVITSDNP
                     RSEDPDAIVDEVLAGTDGHAEREVDRRRAIALTLSGAEPGDVVVIAGKGHEQGQEFEG
                     GRKEPFDDVTVAREALREGVRA"
     gene            complement(13755..15398)
                     /locus_tag="VF752_15115"
     CDS             complement(13755..15398)
                     /locus_tag="VF752_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8856923.1"
                     /translation="MRLVDRRIGLLFGIFLVLLALIGLRAAWLGTVRSGSLSSKALSQ
                     QVENLKVSAQRGTIYDRNGVELAVSEDSVTVFADPLLIKNPVKVAGQIAPLLGRPENE
                     VLKKLSDRSKGFVYLARKIDLSKGQTLEKLKITGIGTTIEPKRRYPQGSLASQVLGSV
                     GTDNWGLSGLEQEFEKTLHGKDGGEKIVKDALGRPVDIVVSKQAERGKDLKLTLDAAI
                     QERTESVLAGVGQTFRPKGATALVMDPRNGEILALANWPRVDANDIGSAPAYARTNRA
                     VAASYEPGSTFKAFTVSGALENKLIAPDTSFDLPPTLQVADRTIKEAHDRGAVTLTTA
                     QILAQSSNIGAVKIGLKLGSTQFDRWVRRFGFGRGTGVDLPGEAAGIVPKPAQYSGSS
                     LGNLPIGQGLAVTPMQMAAAYEAIADGGVIHRPHVVSGMPAEARRVMSATTARQVSKM
                     LEGVLGPGGTAQEAKVPGYELAGKTGTAQKPDPVNGGYSKFKYFASFVGFAPARNPRL
                     LVAVMVDEPQGDIYGGVVAAPAFQKIVSFALPYLKIPPD"
     gene            complement(15402..>15886)
                     /locus_tag="VF752_15120"
     CDS             complement(15402..>15886)
                     /locus_tag="VF752_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856924.1"
                     /translation="PAQRPPARRKSGPARSQVAVALPSLRVPRGAAVLDRLLTGRLWI
                     GCVGVLLVGIVFLNVSVLRLNENIASMSAKADALKRQNAGFRETYAQQSSTERIQRVA
                     VQRGMVLPQPGDVHYLKTKRSDASRAARGMTAPQQQTAPQQQTAPPTASGTSTTGAGG
                     "
BASE COUNT         2333 a         5717 c         5491 g         2345 t
ORIGIN      
        1 cgtcgacacc accacctcgg cgtccggcgg gacgttggcg gcgtcgtgtc cgatgatcgg
       61 ctcgatcccc atctcgcgaa gccgcgcgca ataggacgag tcggccctgt ctgagccggt
      121 cacctcggcg ccgagcgaac gcgccaccaa ggcgaggccg ctcatcccgg cccctccgat
      181 gcctatgaag tgcagccttc tagccgccca gtcgcgatag cccatcgcgc ccggacgata
      241 cctctgccct tagtcagatc gacggcggat tctccggccc cgccgggtcc ggattcgggg
      301 acccggggaa gggcggcttt ggcgccggcg gctcaggatc gggcgccggc gggcgcggat
      361 cgggcaggcc cggtccaccg ggggctcccg gcggtgccgg gtccggcgtg ggtggtgtcg
      421 gcgtctcggc tcgtgttgat cccatgctgc ggccctaccc ggcgaccgga ttactacgcg
      481 tgcgttaaag ggattgcaga aagtgccgga gcgggtcgat gatcgggctg gggaggccct
      541 tcccccgccg gatcggaagc cgacttgcgc ttcgccaaga gactcgcccc cgcccttgcg
      601 ctccttctcc tccttgcctg cggctgcggg caggcgggcc tgtacagcga gcgcgacggc
      661 cccgcgaagc cgaagggcgc ggtcctcgtg atccacggag gagcctggca cgtggtcggc
      721 ccacgggcgg tttactcgat gcgcggctac acggtgcggc tgaaccggct cggctactcc
      781 acgctgaaca tcgactaccg gcgcgggcga cagagcctgc ccgacgtgct ggcggcgtac
      841 gacggcctcc ggcgcgagct cggcccgggc gtgccgatct gcgccctcgg cacctctgcc
      901 ggtggcaacc tggccctgct gctagccgag cgccgctcgg acgtggcctg cgtagtggcc
      961 gaggcgccac ccacgctgat cgccggactc accggtcgcg tgcgcggcta cgcgcgggag
     1021 tacttcccca cgcacgccga catggccgag tacagcccgg ccctccacgt cgacgatctg
     1081 cacgccccgg tgctcgtggc ccaggcgctc cacgacccga cggtgccgta ctcgcaatcg
     1141 agcgccctgg tggcggcgga tccccgcatc aggcgcatag cactgcgccc cggccggacg
     1201 ccctggatcc atgcgaaggt ctatgggccg gacgtcccgc ggctcgacag ggccgaagcc
     1261 gagttcatcg gcgctgcgat cgtcgggtac cgcgggctgc tcgcaagcgg gccgggcggc
     1321 ggcacctgat agaaggggta gccgaatcca cgaaaggagg ccgggaatgg cgtacgaggt
     1381 acctgacctg ccgtacgact acgccgcgct tgagccgcac atcgacgagc agacgatgcg
     1441 gctgcaccac gacaagcacc accagactta cgtcgacaag gcgaacgccg cgctcgaggg
     1501 caccgaatgg gccgacaagc cggtcgagga ggtcctgaag aatttggacc agctgccgtc
     1561 cgacaagcag acgcccgtcc gcaacaacgc cggcggccac tacaaccact cgctcttctg
     1621 gcagatcatg agccccgacg gcggcggaca gcccgacggc gcgctcgccg aggcgatcga
     1681 ctccgccttc ggcagcttcg acgacttcaa gacgaaggtg aacgacaccg gtgccaaccg
     1741 cttcggctcc ggctgggcct ggctcgtgtg ggacggctca ggactggccg tggtggacac
     1801 gccgaaccag gactcgccga tttcgaacgg ccaggtgccg ctgctcggcg tggatgtgtg
     1861 ggagcacgcc tactacctga agtaccagaa ccgccggccc gagtacctcg aggcctggtg
     1921 gaacgtcgtg aactggcccg aggtggcgag ccgcttcgag gccgcccgcg gctgagccgc
     1981 gacccgtggc ccgcggcccc gccggacgcg gggccgctag cgccgcgcgc cggcgatccg
     2041 gtccacgacc agctcgatgt agcggtgctc gtcagggaac gggaacttcg gcatggccgc
     2101 cttggccgtc gccagcccgt gcagcgacat ccagtgcacg caggcgagct cgaatggatc
     2161 cacgtcatcg cccagcagcc gctggaccgc gttgaccagc actccgaacg cattgccccc
     2221 gatcccgagt ccttgcgtca ggtcctccct gcgcttgtct ggtccgccgg gagtctgaaa
     2281 gaggatcgcg tactggcccg gctgctcgcg ggcgaagcgc agataagcgt ggcccatggc
     2341 gatcagctgc gcccgaggat cgccctcgtg ctcggcgtcg gcctctcgca gcacgctggt
     2401 cagtgcctcg aacgagcgcg ccttcacagc ttcggtcagc tcggccttcc ctgagaagtg
     2461 caggtacatg gccgtggggc tcacgcccgc acgcgccgtg accgcacgta cagacagctg
     2521 gtcgacgtcg tgcgtctcgg cgagcagttc gctcgccgcg tcgagtaggg cggtgcgcag
     2581 gcgttctccc tcgccccgct gattgcgaac acgcgtggcc atcgtctcaa ccctatccgc
     2641 cgcacaagcc ggaagagtct tacttgacat cggtacatga acagtgtaca tgtacgaccg
     2701 tcaattgcat acccgtccct ccccccgagg accagccatg ttcacgtcgc tctggcgccg
     2761 cgcagtcagg cgcccccgca gagtcgccgc cctcgccttc gtcgcgttcc tgctcgccgc
     2821 cgtcatcggt ggtcccgcca cgggaagctt cgaggcacct cacgccttcg acgatccggg
     2881 gtcgcagggc acccaggccc gcgaacagat cgagcgcgcc accggcgaca gctcgagcga
     2941 cgtgctcgcc ctggtgcacg ccccgcccgg gagccccgca gtagcgcgca ccgcgagcgt
     3001 cctccgtgcc gaccccgacg tcacgcgcgt gacggtccct tccccgcgcg cgccctcgcc
     3061 gctcgtctcg cgcgatcgcg gcgagaccct gattgcggcc aacgttcgcg cgagcgcgcg
     3121 ggacgaggac cttgtcaagc ggctcgagaa gtcgctgcgc ggcgaccggt cggtctcgct
     3181 gggcggcgac gccgttgcga acacgcaggc cggggagcag gcaacgagcg atctcggaag
     3241 agccgagctg ttcgccttcc cgctgctggc tctgctctcg ctgctggtgt tccgtggcgt
     3301 ggccgcggcg ctcccgctgg cagttggatc gttgagcgtg ctcagcacct tcgccctgct
     3361 ccgtggcgtc aacgaagcgg tcgcgctgtc gccgttcgcc ctcaatctcg tgatcgggct
     3421 cggtctcgga ctggcggtgg actacagcct cctgttcgtc tcgcgcttcc gcgaggagct
     3481 cgggcacggc agcgacgtgt cgcaggccgt gcgccgcacg ctcgcgaccg cgggccgcac
     3541 ggtcgtcttc agcgccgtca ccgtcgcggc agccgccgcg agcctgacgg tcttccccca
     3601 gcgattcctg atctcgatgg gtatcggcgg cgtcatcgtc gcgttggtcg ccgcggcagc
     3661 gaccgttctg gtgctccccg ggctcctcgt gctcctgggc gcgcggatcg gaaaggtcca
     3721 gctcggcccg gatcggagcg ggcgctggta cgggcttgcg cagcgcacga tgcgccggcc
     3781 cgcgctcgtg gccactgcgg cggccgcgat gctgatcgcg ctcgccgccc cggccgtgag
     3841 cctcaactgg accggcgtcg acgcatcgat actccccacc agcaagagcg cgcgcgtggt
     3901 gcaggactcc gtcacgcgcg actttccgca ggcggatgcg agccctgtga acgtcgtgct
     3961 caacgcgcct acgagtgacg gcgcgtcggt gcgtgcctac tcggcgcagc tgaagggcgt
     4021 acagggtgtg cgttcggtga gcactccccg gccgctcgac gcgcgcacct gggagatcga
     4081 cctacacacc ggctccgacg ccgcgtcggg caactcccag cgcgtggtct cggacgtccg
     4141 agcgctgcga gccccggcgc ccgtgttggt cggcggcacc gcggcggacc tgcacgacca
     4201 gcgcgcggcg gtgtcgcatg cggtgccgat cgcactcggc ctgctcgccg tgctcacgat
     4261 cgcggcgctc tggctcatga ccggctcgct cgtgcttccc gccatcgcac tggtgatgaa
     4321 cctgctcacc gtcgcggcgg ccacggggat ccttgtcttc gtgttccagc aagggcatct
     4381 cgccggcctg atcgggacct cggcccagcc cggcatcgag cagaccgact acctcgtgct
     4441 actcgcgatc gttttcgggc tgtcaaccga ctacggcgtg ttcctgctgt cgcggatcaa
     4501 ggaggcgcgt gaccgcggcc tgtccgatca ggaggcgatc gccgtgggcg tgcagcgcac
     4561 cggcgcgatc gtgagcgctg cggcgatcct gctgaccgtg gcgctgggcg cattcgtgac
     4621 cgggaaggtc gtgttcctca aggagctcgg cctcggtgct gcggtggccg tgttgctcga
     4681 cgccttcgtg gtccgcacgc tcctcgtgcc gtcgctgctt cggatcctgg gaccggccgc
     4741 ttggtggtca ccagggccgc tccggcgcct gcacgggcga ataggcgtcg ccgagccggc
     4801 aagcgcctga ccgggccgcc gtgtttcggc gtaggcgacc gggtaaccaa atacgtatgc
     4861 aggcagcacc caagatgctc gtcgtcttcg cgctggcgac gctcggcgta gtcggcatcg
     4921 tgatcgcgct cgcaaccggc cagtggtggg tgctcgcgcc cgccctcctg gcgcatctga
     4981 tcgcgtccgc ggtggccgtc ggctacatcg gcgcccggct cggccaggag gacaagcccg
     5041 atccgatcac agaggcccgg ctacgcgacg agccgcgcgc cgacgcgcgc acgagctcgc
     5101 ccgacgatcg ggacgagccc cacatggcga tctgacatca cgccgcgtcg tggtctattt
     5161 tccggcgcgg tgagggcgtt cgacgtcaag accagcgagc aggacggtgc cgttcgcctc
     5221 gagctctcgg gcgagctcga catctcgagc gcggcccggg ccgaggccga gctgcgccgc
     5281 gccgaggagg cgaagccgga gctgatcgtg ctcgacctgc gacggctcgc tttcatggat
     5341 tccacggggc tccgcctcgt cgtgggcgcg gatctccgcg cccgcgagga aggaaggcgc
     5401 tttgcgatcg tgcagggccc ggacgcgatc cgccgggtgt tcgagatcac ccgcctcgcc
     5461 gagcggctgc agatagtcgc cgatccgtcc gagctctagg cggccacgcg gctgccgttg
     5521 gtgcggtcga gctcgaacca cacgcacgtc acgtgatcgg tctcgacgcc ccagcggtcc
     5581 gccagcgtct cgacgaggta gagcccccaa cccgagccga tgtcgtcctc cgtgccgcgt
     5641 ggcgtggcct cgaatcctgg gccgctgtcg accacctccg cgcgcagcac gtcctcgccc
     5701 accgcgacct cgagccggac ctcgtcgtcc gagccgccag cggcgtgacg cacgctgttc
     5761 gtcaccagct cgctcacgag caggcgcaag tcgtcaagct gcgcagagct cacgtgcggc
     5821 tccagggcat ccagagccag ccgggcggcc gaaaccgcct gcggtccgtt gtcgaggcga
     5881 atcttgaggt ctgtgatcac ggtgctgcct ccgcccgagc acggagatgg tgcgagccga
     5941 acccccggct cgcgcagacc acttacggtg gtaggagcgg atctgctcat ctgcgtggac
     6001 tgtccggaag acccccctgc cagcactctt cctcgcgctt tgcgacgtag acgcctggtc
     6061 gtacccaaag tcacggttcc cacatcggcg aggcggcaat cccacttgag gggtattcgc
     6121 aagagcacat tcacgccata ttcagcgcgg attggcgagc acctcgtcgg cgattcgctc
     6181 cgccgcgtcc ggccgcgcta cgcgccgcgc cgccgccgac atcgccccca accggttgga
     6241 cgagccgagg agcacggcca cctcgcgggc caggcgcggg ccgtcgagct ccgaatcggg
     6301 gatcaccacg gccgcgccct cacgctccat gtagcgcgcg ttgccggtct ggtggttcgc
     6361 cgtcgcgtgc ggatagggga tgaggatcgc cggcacgccc gcggcggcga gctcgaggac
     6421 cgagccaccg gcccgcgccg cggccaggtc cgccaccgcc agcgcgtctg cgaacggctg
     6481 gatgtagggc tcgagccggt agtgctcggg cgagccgagc gcatcgaggc gctcacgtag
     6541 cgacgcgtag tcccgccgcc cgcaggcgtg cagcacggtg cagggcgctg ctcgcccgaa
     6601 tgccgagatc gccgcctcgt tgagcgtgcg cgcaccaagc gagcccccga acacgagcag
     6661 gcagggacgg tcagggcgga tcccgaagcg ctcgtgcgcc gcagcggcgt ccccctcggt
     6721 ggtgccgcgc ggcacgggcc tgccggagag ctcgtagcgc ggcgggcggc gtcgcttcat
     6781 cggaaatgaa aggaaaacgc gctccgcgaa ccgcgcgagc aggcggttcg ccatccccag
     6841 gtggctgtcg gcctcggtga ggtagagcgg gatacgcagc atcacggccg caagcccgac
     6901 cggccccgcc acgtagccgc ctccgccgat cacggccacc ggccgtcgac gccgcaggag
     6961 ccgcagtgcc ttgagggtcg caagcccggc gagcgccacg gcccgggccg ccctcaccgg
     7021 attgcggcga tcgatccccg ccaccggcag gcgcgcgaac tcgtagccgg ccttcggcac
     7081 tagctccgcc tcggcgcgct cgccgcctat gaaggtgacg tcggcgcctc tatcccgtag
     7141 cgcgtcggcg atcgccagcg ccggcacgac gtggccggcc gttccgcccg ccgcgatcgt
     7201 cacccgagcc acgggcgcct ccgtcgatcg ctcgcagcgg ccgcggcctg cttgtcgctc
     7261 gcccggagcc gccggtcctc gccacgttca gcagcacgcc catgccggcg agcatcacga
     7321 tcaggttgct gttgccgtac gagatgaacg gcaacggcac acccgtgagt ggggcgagcc
     7381 cgagcaccgc catgaagttg agcgtggcct gcgagagcac cagcgaggtg agcccggctg
     7441 caaccagctt cgagtagcgg tccttcgcca gcttcgccgc gcgcagcccc gcgtaggcga
     7501 tcaagccgta cagcgccgcg accgccgaga tcccgataag gccgagctcc tcgccgatga
     7561 tcgcgaggat catgtccgtg tgggcctcgg gcaggtagaa gatcttctgc accgactcgc
     7621 cgaggccgcg gccgaacatg ccgcccgagc cgatggcgat catcgactgc accgactgga
     7681 accccgcgcc cgtcgcgtcg tgccaggggt tgacgaacga catcagccgc gcgcgccggt
     7741 aaggctcgac gatcgcgaag atcgtggcga agaagaccag tacgccgagg atcgtgagca
     7801 gatggcggac cggcactccg gcggagatca gcagggcgcc gatcgagaag cagacgacga
     7861 gcgcggtgcc catgtccggc tgcttgagca gaagcaggca agccgccccc acgacgagca
     7921 gcagcgggtt cgcgagctcc ttcaggctgc gcatgcggcg cggctgagtg gtcagcacct
     7981 gcgcggcgta gagcaccagg gacaccttca gcagctccga gggctggaac tgcaggggcc
     8041 cggcgccgag ccagcgggtg gcgccgttca cgttcactcc cactcccggg accatcacgg
     8101 ccagggtgag gaggaaggac gcaccgagca atagcggcgt ggccttgcgc atcacatcga
     8161 gcccgtgccg cgagagcgcg tgcagtaccg ccacgccgat cacggcgaga atcaggtagc
     8221 gcttcaggta gtacgccggg tcgcccggcc cggtgagaag cgactccgcc gagctcgccg
     8281 aatacaccat caccgcgccg gccgcgagca ggcacagcgt ggccgtgtag aggatcgagt
     8341 actcgagtgg tggcttgggt cgtgcggccg cgcgcgagcg agggcgtcgc tcggtctttc
     8401 gcgtggcggc catccgggcc tgcttcgacg cgcacgtcgt gaatcatgct ggacggatca
     8461 gacgggcgcg agggcggtca cgagagctcg aaagtggtcg ccgcgttcct cgaagttgcg
     8521 gtactggtcg tagctcgcgc aggccggcga aagcagcacc acctcgcccg gctgcgcggc
     8581 cgcggcggcc tggctcacgg cggtgccgag gtcgccggaa cgatggagct ccgcggtgcc
     8641 gtcgaggtcg gccgcgaggc gctcggcggc ctctccgatg acgtagacgg cccgagagcg
     8701 gcgcctcacc ggcttgcgca gctccttgaa gccgccgccc ttgaggcttc cgccgaggat
     8761 gaggtgaatg ccgccctcga acgcctcgat ccccacgcgg gcggcgtcca cgttggtggc
     8821 cttggagtcg ttcacgaaca gcacgccgtt cagctcggcg acctcctcga ggcggtgggg
     8881 cacgcccgcg aaggactgaa gagcgcggct caccgcctgc ggctcgattt ctcgcgcgag
     8941 cgccgcggcc gcggccgcca tcgcgttctc gaggttgtgg cgaccgcgga tgcgcacgtc
     9001 ggcggcgcgc atcaggcgcc tacggcgcca ttcgagccag ccgccgcgct gcgcgaggtc
     9061 tgcaccctct cctccgaact ccacgagctc ggcagtaccc ggacggccga agcccctggg
     9121 cacgacggcg acgtcgcggt cgctctggtg cgcgaacgcc tgcagcttgg cggccaggta
     9181 ggcctccatc gtgcggtggc ggtcgaggtg gtcctccgtc acgttgagga gcacggcgca
     9241 ctccggcgcg aagcgcttcg tgtcctcgag ctggaagctc gacgcctcgc acaccacaac
     9301 ggtgtcctcc gccagctcgc ccacgagcgt ggagagcggc gtaccgacgt tgcccgcgac
     9361 cgcaacgggc acgcccgcct cgcggtggat cgcgccgagc agctccgtgg tggtggtctt
     9421 cccgttcgtg cccgtgaccg cgatgaactc gttgggcagc agccgccacg ccagctcgag
     9481 ctcgcccacc accgcgatcc cgcgctcacg cgcggcggcg atcgcagggg cgtcgctcgg
     9541 cacgccgggg ctcttcacca ggcagcgcac gccttcgagc agctcgagcc cgtcggactc
     9601 gagcgcgatg tgcacgcctt cgagcccggc cgcctcgtcg ggccgcccgg agtccgcgcc
     9661 ccgcacgtcg ccatcgccgt gctcgagcag catgcgcgcc gccgcggcgc ccgagcgcgc
     9721 cagccccacg accaggtagg ggccctccgg tagcggcggg cgcccgccca tcagctgatc
     9781 gactgctggt agagcgtgaa gccgatcgcc gcgccgatcg cggcgacgat ccagaagcgg
     9841 aggatgatct tcgtctccga ccacgcgagc agctcgaagt ggtggtgcac gggagccatc
     9901 aggaacacgc gtctgcggaa gcgctggaac gagatcacct ggatcaggac cgagagggcc
     9961 tcgatcacga agatgccgcc ggtgatcagg agcaggacct cagtcttcgt catcaccgca
    10021 agcgccgcga tggcgccgcc taacgcgagc gatcccgtgt cccccatgaa caccgatgcc
    10081 gggaacgagt tgaaccagag gaagccgacg cacgcgccca cgaggcagga gcacaccagc
    10141 gcgaggtcgt cctgtcctgt cgtgatgaac gtcatcgcgg tgtaggcgag gaacacgatc
    10201 gcggcgcagc ccgccgcgag cccgtcgagg ccgtcggtga ggttcacgcc gttcgtcgcg
    10261 cccgcgagca cgagaaagat cagaaccgga tagaggccgt cgatgttcgc cgtgaagtcg
    10321 aagatgcgga accggagcgt gtcggagagc ccgacgccct tcgacgcggc gagccagagc
    10381 ccgatcgcga tgagcagctg aacgacgagc ttcgtgcgac ccgaaacgcc gagcgagcgc
    10441 cgcttcgaga tcttcatcca gtcgtcgacg aagccgagcc cggcgctcgc cagcatcgtg
    10501 ccgaccacgg cgagcgtctc ccagtcgtac tcgcccagga tgaggaacgg cacgaccacg
    10561 gagaacacga tcagcaggcc gcccatcgtc ggagtgcccg ccttcgtgga gtgggctgcc
    10621 gggccctcct cgcggatgtg ttgcccgaac tcgtgcgtct tcagaacgtc gatgaacttc
    10681 ggcccgagga acatgcagat gagcagcgag gccatacccg cgatgaggat cgatccgagc
    10741 aagcgtcagt tccccatcac gcgagcaccc gctccagccc gacggagcgg gagcccttga
    10801 cgagcacgcg gtcgcccggc tgcgcgatct cctcgagcag cgcgccggcc tcctccgggg
    10861 tggcgaccga gtgcgtctcg ccggcgtagc cctgtgcgaa gccgagcccg agctcgccca
    10921 cggtgatcag cacgtcgatg ccgagccggt cggcgtgctc gccgagctcg cggtgccagc
    10981 ggtcggcctc ggggccgagt tcggccatgc cgccgagaat cgcgatgcgt cggctcgcag
    11041 gagtggccgc gaggtggtcg agagccgcgc gcatcgacat cgggttggcg ttgtagcagt
    11101 cgttcacgac cgtcaccccg ccgccgagct cgagcacctc gccgcgcagc gaggagaact
    11161 gcacgtcgac cgagccgcgg ggcgttacgc ccagcgccag gcaggcgccg acagcggcaa
    11221 gcgtgttctc gaggttgtgc ggctcctcga acggcagctc gagctcgatc cgctccccgc
    11281 gtgcgtcgat gagcgcgcgc ccttcgctga acgactcgag cgacacgtcg ccgccctctc
    11341 cgaatgtgat cgtctcgagc tcacctcgca gatgcggctc gagcagcggc tcgtcggcgg
    11401 gcaccacgca ggcgccgccg ttcggcaggt cgcggatcag ctccgccttg gcggccgcca
    11461 cgcgctcgat cgtgccaagc agctccaggt gcacggggcc gacgttcacg atcacgccca
    11521 cctgcggctc ggcgatcgac acgagctcgg cgatctgtcc ctcgccgcgc atcgccatct
    11581 cgaggacgag gacgtccgtg ccgcgctcgg cctggagcag cgagagcggc aagccgattt
    11641 cggtgttgaa gttctcgcgg ttcgagtgca cgcgcttgtg cggccgcagc agcgcggtga
    11701 ggatgtcctt cgtcgacgtc ttgccggtcg agccggtcac gccaaccgcg gggcagtcca
    11761 gttggaggcg ccactcgcgc gcgagcgacg cgagagcggc gagcgggttc tcggcgccga
    11821 tcacgaccgc accgccgacg cgggcgcgcg tcatctgatc ggcccggctc tgctccacta
    11881 gcacgcccca cgcgccctgt tcgagcgccg cgcgcgcgaa ctcgccgccg tcggcattgg
    11941 aacccgggag gccgacaaac aggtcgccgg gccccacctc gcgcgagtcg atcagggcgc
    12001 gccggggtcc cggccggtcg gggtcgccgg cgactaggac gccgccggcc gccctggcga
    12061 cccaagcagc atcgcggtcg atcacgcgcg caccccctcg cggagcgcct cgcgcgcgac
    12121 ggtcacgtcg tcgaacggct ccttgcgccc gccctcgaac tcctggccct gctcgtggcc
    12181 cttgccggcg atcaccacca cgtcgcccgg ctcggcgccc gacagcgtga gcgcgatcgc
    12241 gcggcggcga tccacctcgc gctcggcgtg accgtcggtg cccgcaagca cctcgtccac
    12301 gatcgcgtcc ggatcctcgc tgcgcgggtt gtccgaagtg atcaccacac ggtcagcgag
    12361 cgcgctcgcg acgcgcccca tcttcgggcg cttgccgcgg tcgcgatcgc cgcccgcgcc
    12421 gaacaccaca tgcagccggc cgcgggtgat ctcgcgcgcc gctcgcagca cgttgtcgag
    12481 cgcctccggc gtgtgcgcgt agtccacgag cacggtgaag tcctggccct cgtcgaccgg
    12541 ctcgaaacga cccggcacgc gaccggcccg ttcgagcccg gcgcgcgcgg tgtcggctgg
    12601 cacgccgagc tggcgcacgg ccgcgaacgc ggcgagcgcg ttcgagacgt tgaacagccc
    12661 gggcagccgc gtctcgatct cgacggcgcc gtcgggcgcc tcgatcacga agcgcgaccc
    12721 gctcgggtcg aagcggacgt ctctcgctcg gtaggccgcg tcgttgtcga tcccataggt
    12781 gatcgcgtcg ccgatgtcgc tcacgagccg ccgtccgtac tcgtcgtcga cgttcacgat
    12841 cgatgggccc tcgtcgtcga agaggcggcg cttcgcgagg tagtagtcct ccatcgactc
    12901 gtggaagtcg aggtggtcct gagtgaggtt cgtgaagacc cgggcggcga agcggatgcc
    12961 ctcggcgcgg ccgagcgcga gcgcgtgcga ggacacttcc atcacggccg cccggtcgcc
    13021 cgcgtcgagc atccgacgga acgtgtgctg gaggtcgatc gcctccggcg tggtgcgctc
    13081 gacgggttcc tcctgcccgc ccacgaccga cttgacggtg ccgagcaggc cgctctggcg
    13141 cccgtcggct tcgagcaggt cgcggatgag gaacgccgtg gtcgtcttgc cgttcgtgcc
    13201 ggtgatgccg accacctgca gcttcgccgt ggggtcgccg cagaacttca cggcggccgg
    13261 agccatcgcc gcccgcgcgt cggggacgat cacctcgggc acgccgagat cgagtggccg
    13321 ctccgccacg agggcgacag cgccgcgttc tacggcgtcg ggtgcgaagt catggccgtc
    13381 ggacgtgaag ccgcgcacgc aaaaaaagag cgcccctgga gcgacgctct tgctcgaata
    13441 cgccagcgcg gtgatctcga gatccggcgg cccctgaccc atgagctcgc gcaggtccat
    13501 ggatcggggc agtctagaac gtcgcccgga gcacccgcgc ttgtgtcagt tggcgcaagg
    13561 gcgctcgcag cgcggcgggc gtcgtcactt ggggcccatg gttgccccga atcgacgacg
    13621 cgtgccggcc gcgtgggcga gtgtcgtcac tttgggccca tagtgggccc gaatcgacga
    13681 cgggtaggcg tagagtggct cagcgactcg acgaccggca ttgcacgacc ctaaccgtgg
    13741 gcgtggaccg tgcctcagtc cggcgggatc ttcaggtacg gcagcgcgaa cgagacgatc
    13801 ttctggaacg cgggtgccgc caccacgccg ccgtagatgt cgccctgcgg ctcgtccacc
    13861 atcaccgcca ccagcagccg cgggttgcgc gcgggcgcga agcccacgaa cgaggcgaag
    13921 tacttgaact tcgagtaacc gccgttcacg gggtccggct tctgggccgt gccggtcttg
    13981 ccggcgagct cgtaacccgg caccttcgcc tcctgggcgg taccgccggg gccgagcaca
    14041 ccctcgagca tcttcgacac ttggcgcgcg gtcgtggccg acatcacccg ccgtgcttcc
    14101 gccggcatgc cggagacgac gtgcgggcgg tggatcacgc cgccgtccgc gatcgcctcg
    14161 tacgccgccg ccatctgcat cggagtgacc gcgaggccct ggccgatcgg caggttgccg
    14221 agtgaggagc ccgagtactg cgctggcttc ggcacgatgc ccgcggcctc gcccggcagg
    14281 tcgacgccgg tcccgcgccc gaagccgaag cggcgcaccc agcggtcgaa ctgcgtcgac
    14341 ccgagcttca ggccgatctt caccgctccg atgttcgacg actgcgccag gatctgcgcg
    14401 gtcgtgagcg tgaccgcgcc gcggtcgtgc gcctccttga tcgtgcggtc ggccacctgc
    14461 agcgtcgggg gcagatcgaa gctcgtgtcg ggcgcgatca gtttgttctc gagcgcgccg
    14521 gacacggtga acgccttgaa ggtcgagccc ggctcgtagc tcgccgccac ggcacggttg
    14581 gtgcgcgcgt acgccggcgc ggagccgatg tcgttcgcgt ccacgcgcgg ccagttcgcg
    14641 agggcgagga tctcgccgtt ccggggatcc atcacgagag cggtcgcgcc cttcggccgg
    14701 aacgtctgcc ccacgccggc gagcacggac tcggtgcgct cctggatcgc cgcatcgagc
    14761 gtcagcttca ggtccttgcc gcgctcggcc tgcttcgaga cgacgatgtc gaccggccgc
    14821 ccgagcgcgt ccttcacgat cttctccccg ccgtccttgc cgtgcagggt cttctcgaac
    14881 tcctgctcga ggccggacag cccccagttg tccgtgccca ccgaaccgag cacctgggag
    14941 gcgagcgagc cctgcgggta gcgccgcttc ggctcgatcg tcgtgccgat gccggtgatc
    15001 ttcagcttct cgagcgtctg gcccttgctg aggtcgatct tccgggcgag gtagacgaac
    15061 cccttgctgc ggtcgctgag cttcttcagc acctcgttct cgggccggcc gagcagcggc
    15121 gcgatctgcc ccgcgacttt caccgggttc ttgatcagga ggggatcggc gaagacggtt
    15181 acggagtcct cggagacggc cagctccacg ccgttgcggt cgtagatagt gccgcgctgc
    15241 gccgacacct tcaggttctc gacctgctgc gagagcgcct tgctgctgag gcttccagag
    15301 cgcaccgtgc cgagccaggc ggcgcggagg ccgatcaatg cgaggaggac cagaaagatc
    15361 ccgaacagca ggccgatgcg gcgatcgacg agccgcacga gctagccgcc ggcccccgtt
    15421 gtgctcgtgc cagacgccgt tggcggcgcg gtctgctgct gcggcgcggt ctgctgctgc
    15481 ggcgcggtca tcccgcgggc cgcccggctc gcgtccgaac gcttcgtctt gaggtagtgg
    15541 acgtcgcccg gctgcggcag caccatgccg cgctgcacgg cgacgcgctg aatgcgctcc
    15601 gtcgacgact gctgcgcgta ggtctcgcgg aaccccgcgt tctgccgctt gagcgcgtcg
    15661 gccttcgcgc tcatgctcgc gatgttctcg ttcaaccgca gcacgctcac gttgaggaac
    15721 acgatcccga cgagcagcac gccgacgcag ccgatccaga gccggccggt gagcaggcgg
    15781 tcgagcacgg ccgcgccgcg agggacgcga aggctcggaa gtgcgacggc gacctgggag
    15841 cgcgccggcc cggacttgcg tcgcgccggg ggccgctgcg ctgggc
//
LOCUS       DASVXS010000266         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_684338, whole genome shotgun sequence.
ACCESSION   DASVXS010000266 DASVXS010000000
VERSION     DASVXS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684338"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1444)
                     /gene="obgE"
                     /locus_tag="VF752_15125"
     CDS             complement(143..1444)
                     /gene="obgE"
                     /locus_tag="VF752_15125"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX8856925.1"
                     /translation="MLYDRAKIFVQAGAGGSGVVSFRREAHVPRGGPDGGDGGRGADV
                     VLVVDPSRRDLQSFKRKAHYKARRGGHGEGSNRHGASPEALEVHVPPGTVVEDVDGTM
                     RFDLVEPGQRVVVASGGSGGRGNKRFAHSTRQAPRFAERGLPGEERWLELRLKLLADA
                     GLVGLPNAGKSSLLARMTRAHPKVADYPFTTLEPVLGTLEGEDRQIVVADIPGLIEGA
                     GEGAGLGHEFLAHVERTRLLVHVLDLAPLDGSDPVENFETVEAELETYGAGLAELPRI
                     LALSKADLVPAEEAERSAADWRERLGERVIDVIVTSAATGQGVDELAASLLRNVPEHA
                     PAAAEAEVELAEHRVYRPADDEGFRVERTPEGTFRVAGPRVDRLIARHDLENEDAMRY
                     VEERLRAIGVIRALEAAGFEPGDDVELGGVVFELDPGAPFR"
     gene            complement(1447..2328)
                     /locus_tag="VF752_15130"
     CDS             complement(1447..2328)
                     /locus_tag="VF752_15130"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX8856926.1"
                     /translation="MTYSLVARDAETGELGVAVQSHYFSVGSIVPWAEPGVGAVATQS
                     MVEVSYGPRGLELMRAGRRPFEALDELVRQDPAADVRQVAMVNAAGEVAVHTGPRCIR
                     EAGHASGEGVSCQANMMRRDSVWGAMLAAFESASGDLASRMLDALDAAEAEGGDIRGR
                     QSASLLVVSPERGLDLRVEDDPAPLAELRRLLELRRAYEHVDRGDERVAAGDLDGAMR
                     EYARALELAPDNVEMKFWTGVTLAAGGRVDEARPYLDDAFAAGDGWAELLRRLPASGL
                     FPEDPDLLHRTLGHTGP"
     gene            complement(2341..2598)
                     /gene="rpmA"
                     /locus_tag="VF752_15135"
     CDS             complement(2341..2598)
                     /gene="rpmA"
                     /locus_tag="VF752_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX8856927.1"
                     /translation="MAHKKGLGSSRNGRDSQAQRLGVKIFAGQVVNGGEIILRQRGTK
                     FKPGDGAGIGKDDTIYARRAGTVEFTTGRRGRVVSIAPNGD"
     gene            complement(2588..2953)
                     /gene="rplU"
                     /locus_tag="VF752_15140"
     CDS             complement(2588..2953)
                     /gene="rplU"
                     /locus_tag="VF752_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX8856928.1"
                     /translation="MYAIVKVGGKQYRVEKGDTLLVDRMREDEGAKLTLEPLLFADGD
                     KSVFEGADLQKVKVEAVVAGHERGKKVRVVKFKPKRGYKLTRGHRSELTRLEVSDIKM
                     LSRKPAAKKSESKETEDGS"
     gene            complement(3009..>4303)
                     /locus_tag="VF752_15145"
     CDS             complement(3009..>4303)
                     /locus_tag="VF752_15145"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX8856929.1"
                     /translation="QRRAAETLAPAMVFQEADLPVRVVRDIFSSQFEEALVDDEKAYN
                     RLVSFLTRTAPELVDRVELVPKGEGDRLFERLGIEDVIQSTLSRRVDLPSGGYLMIDY
                     AEAMTVIDVNSGSFIGRGKGAKLEDTITKTNLEAAEEVVRQLRMRDIGGIIVIDFIDM
                     ARARNRDAVLKTLRKELDEDRTKTYVMEISPLGLVEMTRQNVTDGVREIITKTCPTCH
                     GEGVVLSEETVAIEVERKLRELATERPDAEAFLVQVHPRVSQVLLSGPGTPPLLELED
                     EVGKHFHFEGSEGLPIDHFAVSAEGSREEIEERALPFREGEEVFVTIEEPHMYNEDDA
                     VAKIDGYVISVSNAVRQIGKQVLVRIDNVSRTSASAQIVRQNGAGPEPEAEAEESAEE
                     SAETGSTGRRRGRRGGRRRRGSRAGAPPEEAAAAEPSD"
BASE COUNT          607 a         1615 c         1412 g          669 t
ORIGIN      
        1 agctatgtcg cctccgcccc agaacacgaa cgggagcgtg tcgcccgcga ggtgcgcggg
       61 gcggtcgcac gccacgccgg cgactccgag cacgtgcgga tcgcctacac aacgacggtc
      121 tactggcgcg tgcgcagcgc cgctacctga acggcgcgcc cgggtcgagc tcgaacacca
      181 cgccgccgag ctccacgtcg tcgcccggct cgaagcccgc ggcctcgagc gcgcggatca
      241 cgccgatcgc tcgcagccgc tcctccacgt agcgcatcgc gtcctcgttc tcgaggtcgt
      301 ggcgagcgat caggcggtcg acgcgcgggc ctgcgacgcg gaatgtgccc tccggggtgc
      361 gctcgaccct gaagccctca tcatccgccg ggcggtacac gcggtgctcg gcgagctcga
      421 cctcagcctc tgcggccgcc ggcgcgtgct cgggcacgtt gcggagcagc gaggccgcga
      481 gctcgtccac gccctggcca gtcgcagccg aggtgacgat cacgtcgatc acgcgctcgc
      541 cgagccgctc gcgccaatcg gccgcggagc gctcggcctc ctccgccggc acgaggtcgg
      601 ctttcgacaa agcgaggatg cgcggcagct ccgcgaggcc ggcgccgtag gtctcgagct
      661 cggcctccac cgtctcgaag ttctccaccg gatccgagcc gtcaagcggc gccaggtcga
      721 gcacgtgcac gagcaggcgc gtgcgctcca catgcgcgag gaactcgtgg ccgaggccgg
      781 cgccctcgcc cgcaccctcg atcaggccgg gaatgtcggc caccacgatc tgccggtctt
      841 cgccctcgag cgtgccgagc accggctcga gcgtggtgaa cgggtaatcg gccaccttcg
      901 gatgcgcgcg cgtcatgcgc gcgagcagcg acgacttgcc ggcgttcggg agaccgacga
      961 gaccggcgtc ggccagcagc ttcaggcgca gctcgagcca gcgctcctcg cccggtagac
     1021 cacgctcggc gaagcgaggc gcctggcgcg tggagtgcgc gaagcgcttg ttgccgcggc
     1081 cgcctgaacc gccgctcgcc accaccacgc gctgccccgg ctcgaccagg tcgaagcgca
     1141 tcgtgccgtc cacgtcctcg acgaccgtgc ccggaggcac gtggacctcg agcgcttcgg
     1201 ggctcgcccc atggcggttc gagccctcgc cgtgcccgcc gcgccgcgcc ttgtagtgcg
     1261 ctttgcgctt gaacgactgg aggtcacgcc gcgagggatc caccaccagc acgacgtccg
     1321 cgccgcggcc gccgtcgccg ccgtcgggcc cgccacgcgg cacgtgcgcc tcacggcgaa
     1381 acgacacaac gccggagcct ccggcacccg cctgcacgaa gatcttcgcc cggtcgtaga
     1441 gcatggttac ggccccgtat gcccgagcgt ccggtgaagc aaatccgggt cctcggggaa
     1501 tagccccgat gccggcagcc gccgcagcag ctcggcccag ccgtcgcccg cggcgaacgc
     1561 gtcgtcgaga tacgggcgtg cctcgtccac gcgaccgccc gcagccagcg tcacgccggt
     1621 ccagaacttc atctccacgt tgtcgggcgc cagctcgagc gcacgcgcgt attcgcgcat
     1681 cgcgccgtcg aggtcaccgg cggccacgcg ctcgtcgcca cgatccacgt gctcgtaagc
     1741 gcgacgcagc tcgagcaggc ggcgcagctc cgcaagcggc gctggatcgt cctccacgcg
     1801 caggtcgagc ccgcgctccg gcgagaccac gagcagcgac gccgactggc ggccgcggat
     1861 gtcgccgccc tccgcctccg cggcgtcgag tgcgtcgagc atgcgtgaag cgagatcgcc
     1921 cgaggcactt tcgaaggcgg cgagcatcgc tccccagacg ctgtcgcggc gcatcatgtt
     1981 ggcctgacac gagacaccct ctccgctggc atggcccgcc tcgcggatgc agcgcggacc
     2041 ggtgtgcacc gccacttcgc cggcggcgtt caccatcgcc acctggcgca cgtcggcggc
     2101 gggatcctgc ctgacgagct cgtcgagcgc ctcgaacggc ctgcgccccg cgcgcatcag
     2161 ctcgagcccg cgcgggccat acgacacctc gaccatcgac tgtgtcgcca cggcgcccac
     2221 gccaggctcg gcccacggga cgatcgagcc caccgagaag tagtgcgact gaaccgcgac
     2281 accgagctcg ccggtctccg cgtcgcgcgc gaccagcgaa taggtcatcg gggcggtggg
     2341 ctagtcgccg ttcggcgcga tcgagacgac ccggccgcgg cgaccggtgg tgaactccac
     2401 cgtgcccgcg cggcgagcgt agatcgtgtc gtccttgccg atgccggcgc cgtcacccgg
     2461 cttgaacttg gtgccgcgct ggcgcaagat gatctcgccg ccgttcacga cctgacccgc
     2521 gaagatcttc acgcccagtc gctgtgcctg cgagtcacgg ccgttgcggc tcgatccgag
     2581 gcccttctta tgagccatcc tcggtctcct tcgactccga cttcttcgcg gcgggcttgc
     2641 gggagagcat cttgatgtcg ctcacctcga ggcgggtgag ttccgaccgg tgtcccctcg
     2701 tgagcttgta gccgcgcttt ggcttgaact tcacgacccg caccttcttg ccgcgctcat
     2761 gacctgccac gaccgcctcg accttcacct tctgaaggtc ggcgccctcg aagacggact
     2821 tgtcgccgtc cgcgaacagc agcggctcga gggtgagctt cgctccctcg tcctcgcgca
     2881 tgcggtcgac gagaagggtg tcgcccttct cgacccggta ctgcttgccg cccactttca
     2941 ctatcgcgta catggcagga aaagggcgcc ctccgggggc gccggactag gagtgtagcg
     3001 tggcccggct aatccgaagg ctccgccgcg gcagcctcct ccggcggcgc tccggcgcgc
     3061 gatccgcgcc gcctgcgccc acctcggcgc ccgcgcctgc ggcccgtgga acccgtctcc
     3121 gccgactctt ctgcgctctc ctcggcctcc gcctccggct cgggaccggc gccgttctgg
     3181 cgcacgatct gggctgaggc cgacgtgcgg ctcacgttgt cgatccggac cagcacctgc
     3241 ttgccgatct gcctgaccgc gttcgacacc gagatcacgt agccgtcgat cttcgcgacc
     3301 gcgtcgtcct cgttgtacat atgcggctct tcgatcgtga cgaagacctc ttcgccctcg
     3361 cggaacggca gcgcgcgctc ctcgatctcc tcgcggctgc cctcggcgga caccgcgaag
     3421 tggtcgatcg gcaggccctc ggagccctcg aagtggaagt gcttgcccac ctcgtcctcg
     3481 agctcgagca ggggtggcgt gcccggtccg ctgaggagca cctgcgagac gcgcgggtgc
     3541 acctgcacca ggaacgcttc ggcgtcgggt ctctcggtgg cgagctcgcg gagcttgcgc
     3601 tcgacctcga tcgcgacagt ctcctccgac agcacgacgc cctcgccgtg gcacgtcggg
     3661 caggtcttgg tgatgatctc ccgcacgccg tcggtcacgt tctggcgggt catctcgacc
     3721 aggccgagcg gcgagatctc catcacgtac gtcttggtgc ggtcctcgtc gagctccttg
     3781 cgcaacgtct tcagcacggc gtcgcggttg cgcgcccgcg ccatgtcgat gaagtcgatg
     3841 acgatgatgc cgccgatgtc gcgcatcctc agctggcgca cgacttcctc agcggcctcg
     3901 aggtttgtct tcgtgatcgt gtcctcgagc ttcgcgccct tgccgcgccc gatgaaggag
     3961 cccgagttga cgtcgatcac ggtcatcgcc tcggcgtaat cgatcatcag gtagccgccc
     4021 gaggggaggt ccaccctgcg actgagcgtc gactggatca cgtcctcgat ccccaggcgc
     4081 tcgaacaggc ggtcgccctc gcccttcggt acgagctcga cgcggtcgac cagctcgggc
     4141 gcggtgcgcg tgaggaacga gacgaggcgg ttgtaggcct tctcgtcatc cacgagcgcc
     4201 tcttcgaact gcgacgagaa gatgtcgcgc accacgcgga ccggaaggtc ggcctcctgg
     4261 aacaccatgg ccggcgcgag agtctccgcg gcgcgccgct gca
//
LOCUS       DASVXS010000267         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_684688, whole genome shotgun sequence.
ACCESSION   DASVXS010000267 DASVXS010000000
VERSION     DASVXS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684688"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VF752_15150"
     CDS             complement(<1..1281)
                     /locus_tag="VF752_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8856930.1"
                     /translation="MTTETIAPARTVASIIGGRPRTDAPGGRLVSTNPAHTDDVVAEV
                     LLGDAQTFVDACGAAREAQKAWAATPAPIRGRAIQQIGRLVEDNKEALARLVTREIGK
                     PYPESLGEVQEVIDTCNFFVSEGRRLYGQTVPSEMPDKQLFTFRTPVGVAAIVTAGNF
                     PVAVPSWYLVPALLCGNAVVWKPAEYAPALGEALSQLFLHGGLPDGVFNLVQCEGAVA
                     FEGIEQALEQGLVDKVGFTGSSDVGRKIGELCGRHLQQPCLELGGKNPLVVMPDADLA
                     LAVEGALFSGFGTAGQRCTSLGTAIVHDSVYDDFVGQLTAAVESAPIGDPTRDVLYGP
                     MIHERFLDRFLGWLELIQDHHRVSGSTGTGRITADNPRDGFVGDPEAGLFCHPTIVSG
                     VMIDDEIYRTETFGPIIGVAKFSDFDEAMELANNH"
     gene            complement(1532..1726)
                     /locus_tag="VF752_15155"
     CDS             complement(1532..1726)
                     /locus_tag="VF752_15155"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX8856931.1"
                     /translation="MGRKFMDCREMPSESNCSLYISGEEEEVVRAAAEHAASVHGHED
                     TPEFREQIRTQLKDEVTVPA"
     gene            1836..2438
                     /locus_tag="VF752_15160"
     CDS             1836..2438
                     /locus_tag="VF752_15160"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8856932.1"
                     /translation="MTQVAAPTRERIVEAATRLFAEKGYRDTTVGEIEEAAGLAPRRG
                     ALYKHFSSKEELFSAAVEIESRKLDDIELVTAPSGSGDLREELLLIGRWSLRRLAGER
                     VLLQVVEREGHRFPQLTRDFYERIVQRGHNEAVAWIRAHVETGALPDMDVEARAVVAV
                     ASLVNYERMRAMFGEVPLGVDEERFLEAWVESMIGNGGSP"
     gene            2435..3487
                     /locus_tag="VF752_15165"
     CDS             2435..3487
                     /locus_tag="VF752_15165"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8856933.1"
                     /translation="MTREIRGLGDARRVLRWGLVALALMNAPVGVWATISPHSFYTGF
                     PGAGRHWVSALGPYDEHLVRDVASLELAITVLFLLAAVWLTRRLAQAALIASLFWALP
                     HFLYHLATLDRYGLGDSIGNVVGLGAELLLPLALLWVVPRAFAPAAPGAAAPPAPGER
                     GRIAPAPERGLWNRLTYGYARREAAGTVPDPVRVFAHNRKISVGYGAIEKAFAGSHRV
                     DERLKTLAELRAASIAGCEWCLDFGSALAKGADISQEKLMALPRYGESQLFEADERLV
                     LDYATAMSRSPVEVSDELFERLRARFDEAQLVELTGIIALENFRARFNDAVGLGAQGY
                     SDGSFCVLPEREAVTR"
     gene            3571..3771
                     /locus_tag="VF752_15170"
     CDS             3571..3771
                     /locus_tag="VF752_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856934.1"
                     /translation="MTNEQITRNASAIAGAVKMLEREIASFVSAIDAEGLDDEGARTI
                     SGYLTELHQHFSALTEHADRLE"
     gene            3799..>4711
                     /locus_tag="VF752_15175"
     CDS             3799..>4711
                     /locus_tag="VF752_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8856935.1"
                     /translation="MARALPMLLVLAALALPAAGAAAAAPLAIKDGRIVDRATGATVV
                     LRGVNVVYKPAPYLPHGSSERTSFDRQDVRRLRSWGFNAIRLGVTWKALMPTRGHVSR
                     TYIAQVAKLARVAERGGLYVLIDMHQDLWSEQFQGNGAPAWATITDGLPFKKRKNPFP
                     LNYLQPPVGRAFTSFFENRDGIRTLYVRAYAALARKLRGDTGVVGFDVMNEPICEVAK
                     APCGLPVPPEAAGKWLQPFYDELIPALKTAAPRRAAFYEDAVSVNAGFPFLLGYPPNK
                     AWPFPGEALSFHVYCNGIPRPGVPCETQ"
BASE COUNT          703 a         1648 c         1626 g          734 t
ORIGIN      
        1 gtggttgttc gcgagctcca tcgcctcgtc gaagtccgag aacttcgcga cgccgatgat
       61 cggtccgaac gtctcggtcc ggtagatctc gtcgtcgatc atcacgccgc tgacgatcgt
      121 cgggtggcag aacaggccgg cttccgggtc gccgacgaag ccgtcccgcg ggttgtccgc
      181 ggtgatacgc ccagtgcccg tcgagccgga caccctgtga tggtcctgaa tcagctcgag
      241 ccagccgagg aagcggtcga ggaaccgctc gtggatcatc ggtccgtaga gaacgtcgcg
      301 agtcgggtcc ccgatcggcg ccgactccac ggccgccgtg agctgaccga cgaagtcgtc
      361 gtagacggag tcgtgcacga tcgcggtacc cagcgacgtg cagcgctggc ccgccgtacc
      421 gaagcccgag aagagcgcac cctcgaccgc tagcgcgagg tcggcgtcgg gcatcaccac
      481 gagcgggttc ttgccgccga gctcgaggca cggctgctgt aggtggcggc cgcagagctc
      541 gccgatcttg cggccaacgt cgctcgagcc ggtgaagccc accttgtcga cgaggccctg
      601 ctcgagcgcc tgctcgatgc cctcgaacgc caccgcgccc tcgcactgca cgaggttgaa
      661 cacgccatcg gggaggccgc cgtgcaggaa cagctgcgac agcgcctcac cgagtgcggg
      721 tgcgtactca gcgggcttcc agacgaccgc gttgccgcac agcaacgccg gcacgaggta
      781 ccacgacggc accgcgacgg ggaagttgcc ggccgtcacg atcgcggcca ccccgaccgg
      841 agtgcggaac gtgaagagct gcttgtccgg catctcgctc ggaaccgtct gtccgtacag
      901 ccggcgccct tccgacacga agaagttgca ggtgtcgatg acctcctgaa cttcgccgag
      961 cgactccgga tacggcttgc cgatctcgcg ggtgacgagg cgcgcgagcg cctccttgtt
     1021 gtcctcgacc aggcggccga tctgctggat cgcgcgccca cggatcggag cgggcgtcgc
     1081 ggcccacgcc ttctgcgcct cgcgcgccgc gccgcacgcg tcgacgaacg tctgcgcgtc
     1141 gcccagcagc acctctgcga cgacgtcgtc ggtgtgcgcg ggattggtcg agacgaggcg
     1201 gccgcccggc gcgtcggtac gcggccgccc accgatgatt gaggcgacgg tgcgcgcggg
     1261 ggcgatggtc tcggtcgtca actgaagctc acctctcctc gttgaaaaag gtcggcttca
     1321 tcgtagtggc gggaaccggc gcagaagcgc ccgcgcgctg gcaatatccg cgccactcag
     1381 gcaggggtct gcggaatgct cggggcgaag ttgtgcgccg tgacgctcgc ggggtgcggc
     1441 cggacagaga ccttccccag ccactaggcc aaggaaggaa cgagggccgg cgggcggcgg
     1501 gacgcccgcc ggtgcctcgt cgtcccgcct cttaggcggg gaccgttacc tcgtccttga
     1561 gctgggtccg gatctgctcg cggaactccg gcgtgtcctc gtgcccgtgc actgacgcgg
     1621 cgtgctcggc ggcggcgcgg accacctctt cctcttcccc tgagatgtac agcgagcagt
     1681 tgctctcgct cggcatctcg cggcagtcca tgaacttacg gcccatacag acctcctgac
     1741 tcgagtctcc ctccttctac tcccgggcgg ggaacggagt caagccggct ttaaccggct
     1801 tgcacgaagg taatccgctg attaccatgc ccgcgatgac gcaggtagcc gccccaacgc
     1861 gcgagcgcat cgtcgaggcc gcgaccaggc tcttcgccga gaagggctat cgcgacacca
     1921 cggtcggcga gatcgaggag gcggccggcc tggcgccacg tcgcggcgcg ctctacaagc
     1981 acttctcgag caaggaggag ctgttctccg ccgcggtcga gatcgagtcg cggaagctcg
     2041 acgacatcga gctcgtgacg gcgccgagcg gctccggcga cctgcgcgag gagctcctgc
     2101 tgatcggccg ctggtcgctg cggcgactgg ccggcgagcg cgtgctgctg caggtggtgg
     2161 aacgcgaagg ccaccgcttc ccgcagctca cacgcgactt ctatgagcgc atcgtgcagc
     2221 gtggccacaa tgaggcggtc gcctggatcc gcgcgcacgt ggagaccggc gcgctcccgg
     2281 acatggacgt ggaggcgcgc gccgtcgtcg ccgtcgcgtc gctcgtgaac tacgagcgca
     2341 tgcgcgcgat gttcggcgag gtccctctcg gcgtggacga ggagcgcttc ctggaggcct
     2401 gggtggagtc gatgatcggc aacggaggtt caccatgacc agagagatcc gcgggttggg
     2461 agacgcccgg cgagtgctcc gctgggggct cgtcgcgctg gcgctgatga acgcccccgt
     2521 aggcgtgtgg gccacgatca gcccgcactc gttctacacg ggcttcccgg gagccggccg
     2581 tcactgggtc tccgcgctcg gcccctacga cgagcatctc gttcgcgacg tcgcgtcgct
     2641 cgagctcgcc atcaccgtgc tgttcctgct tgcggccgtt tggctcacgc gccgactcgc
     2701 gcaggcggcg ctgatcgcgt cgctcttctg ggcgctcccc cacttcctct accacctcgc
     2761 aacgctcgac cggtacggcc tcggcgacag catcggaaac gtcgtgggcc tcggtgcgga
     2821 gctgctgctg ccgctcgcgc tgctctgggt ggtgccgcgt gcgttcgcgc ccgcggctcc
     2881 gggagcggcc gcgccacccg cgccgggcga gcgcggccgt atcgcgcccg cgccggagcg
     2941 cgggctgtgg aaccggctca cctacggcta cgcgcggcgc gaggccgcgg gcaccgtgcc
     3001 ggacccggtg cgcgtgttcg cacataaccg caagatctcc gttggctacg gcgcgatcga
     3061 gaaggccttc gcgggctcac accgtgtcga cgagcgtctc aagacgctcg ccgagctgag
     3121 ggcggcgtcg attgcgggct gcgagtggtg cctcgacttc ggctccgcgc tcgcgaaggg
     3181 cgccgacatc tcgcaggaga agctgatggc gctcccgcgc tacggggaga gccagctgtt
     3241 cgaggccgac gagcggctcg tgctcgacta cgcgaccgcg atgtcgcgca gcccggtcga
     3301 ggtgagcgac gagctgttcg agcgcctccg cgcccgcttc gacgaggccc agctcgtgga
     3361 gctgaccggc atcatcgctc tcgagaactt ccgcgctcgc ttcaacgacg cggtgggcct
     3421 cggggcgcag ggctactcgg atggcagctt ctgcgtgctg cccgagcggg aggccgtgac
     3481 gcgctagttc aaggtcacgt ctggcgtgcc gatgtttgct ggtgacggcc gagccccgcg
     3541 ccgtcgcgag actcaacaga aggagcccgc gtgaccaacg agcagatcac tcgcaacgcc
     3601 tcggccatcg ccggcgccgt gaagatgctc gaacgcgaga tcgcgagctt cgtcagcgcg
     3661 atcgacgcgg agggcctcga cgacgagggc gctcgcacga tctccggcta cctgacggag
     3721 cttcaccagc acttctcggc gctcaccgag cacgccgacc gcctggagta gcccggagcg
     3781 ttggtagcgt gcgcgcctat ggctcgcgcg ctgccgatgc tgctggtcct cgcagcgctt
     3841 gcgcttcccg ccgccggcgc cgccgccgcc gcgccgcttg cgatcaagga cggccgcatc
     3901 gtggaccgcg cgaccggcgc gaccgttgtg ctccgcggcg tgaacgtggt ctacaagccg
     3961 gcgccctacc tgccgcacgg ctcctcggag cgcacgagct tcgaccgcca ggacgtgagg
     4021 cggctccgct cgtggggctt caacgccatc cgcctcggcg tcacgtggaa ggcgctgatg
     4081 cccacccgcg gccacgtctc gcgcacctac atcgcgcaag tcgcgaagct cgcccgggtc
     4141 gctgagcgcg gcgggctcta cgtgctcatc gacatgcacc aggacctctg gtcggagcag
     4201 ttccagggca acggcgctcc agcgtgggcg acaatcacag acgggctgcc tttcaagaag
     4261 cgcaagaacc cgttcccgct gaactacctg cagccgccgg tgggtcgtgc gttcacgagc
     4321 ttcttcgaga accgcgacgg gatccgcacg ctgtacgtgc gcgcgtacgc ggcgctcgcg
     4381 cgcaaattgc gcggtgacac cggcgtggtc ggcttcgacg tcatgaacga gccgatctgc
     4441 gaagtagcga aggcgccgtg cgggctgccg gtgccgcccg aagccgccgg caagtggctg
     4501 cagccgttct acgacgagct gatccccgcg ctcaagacgg ccgcgccgag gcgcgccgcc
     4561 ttctacgagg acgccgtctc agtgaatgcc ggcttcccgt tcctactggg gtacccgcca
     4621 aacaaggcct ggccgttccc gggcgaggcg ctcagcttcc acgtctactg caacggaatc
     4681 ccacggccgg gggtgccgtg cgagacgcag g
//
LOCUS       DASVXS010000268         4370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1251223, whole genome shotgun sequence.
ACCESSION   DASVXS010000268 DASVXS010000000
VERSION     DASVXS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251223"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VF752_15180"
     CDS             <1..1123
                     /locus_tag="VF752_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX8856936.1"
                     /translation="GGGAEKPKMEVAVQDDAVLLQRAYYDRNKALDQARDLEVTRIRT
                     LVIWGRVPGSQPDSTTPPAGGRPTYDFSAWDSLIDDAAKRGIRVQLDLTGPVPAWAAG
                     DRKVGVTRPDAQKFGVFAEQAARHFKGRVDRYSIWNEPNYGSWLKPQTEAADLYRNLY
                     LAAYKGIKRADPAAHVLIGETSPYELAGRAIAPLKFLRDVTCRTTIYAPAKRCPTLVA
                     DGYAQHPYDFVSPPERPHPGADNVTIGSLDRLTRALDKLAEGKALTTPAGKPLDMYLT
                     EFGYFASGPAAPPPKQRASWLARAFDIASRNPRVREMLQYILVSPPNKVRFDTGVITP
                     QGQPTAAYTALRDWAHKAAAAGSIDKPDGPIELHTRPAS"
     gene            1163..2449
                     /gene="ispG"
                     /locus_tag="VF752_15185"
                     /gene_synonym="gcpE"
     CDS             1163..2449
                     /gene="ispG"
                     /locus_tag="VF752_15185"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX8856937.1"
                     /translation="MASRRQINVGGVPIGGGAPVAVQSMTKTETADYDATMRQIRAIA
                     DAGGDLVRCAVPRDQDVEALSRIVKESPIPVIADIHFNYTLALKAIDAGAHCIRLNPG
                     NIGGPDKVAQVVEKAKAAGVPMRIGVNSGSLPKHLHALERENSVEALVTAAVEFVELM
                     ERLEFENFKVSIKSTSVPNTIASNRLLSERIDYPLHLGITEAGTKWSGSLKSAVGLGT
                     LLADGIGDTIRISLSTFHAEEEVKVAWEILKALKLREKGPVLIACPTCGRLQFDMDTV
                     VAEIERRLEAYDDPVEVAVLGCAVNGIGEASHADFGITGAKNEGLIFSRGKPLKKVPQ
                     ERLVDELFAEIDKYVAAGRTEVDAKKAAEGAEWLERIEEENAGELTPERLAKLEAEQA
                     RAENGEGNGAVELPQVKKVALDEDVSPTAGRRFTRA"
     gene            2501..3949
                     /locus_tag="VF752_15190"
     CDS             2501..3949
                     /locus_tag="VF752_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856938.1"
                     /translation="MLKNTKRFTFAALLAVSALATTAGAAQASKTQESIFQDDRVLVS
                     SGPGVQTSALDQLQALGVDTIHSLVFWNKIAPSSDSSKKPSFDASNPAAYPAGTWDAY
                     DDLVRGAKARGIDLLLSPTGKSPSWANGCRGKANCQPSAKEFGAFVKAVAKRYSGTYH
                     DENQGGGTLPKVGRWSIWNEPDQGGWIFPQRKSGVVYSAKIYRDLYTAGYSALKATGH
                     GRDKIYIAETAPVRGGVATAPVEFLQALFCIDKNGRKLRGKAAKTLGCTKYKTLKFTA
                     VAHHPYNRGAGGPPLKKPKTAGDITLATLDRLFTVLKQAKRAHRISGSTSSVYFTEYG
                     IQSRPPEQKFGVPLTQHAAQLNQAEYVGYNDRRVKSFSQYELFDEPDVGTFNTGLLFT
                     NGTAKPALAAYRLPIYAVKSGSKVKLWGAVRPGNGSRSVDVLAGGKKVKTVTVRGAGY
                     FTTTVSKASSYQLSATIGGQTFKSRVAKAASR"
     gene            3987..>4370
                     /locus_tag="VF752_15195"
     CDS             3987..>4370
                     /locus_tag="VF752_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856939.1"
                     /translation="MTRLSNYLLPTVKEAPAEAEAISHKLLVRAGLARQLSSGLWTYL
                     PAGWRVHRKVEQIIRVEIDAIGGQEMLMPVLQPADIWKTSSRYSIEELFKLKDRKGAD
                     LVLAMTHEEALTWHMAREIRSYRDLP"
BASE COUNT          784 a         1507 c         1496 g          583 t
ORIGIN      
        1 gggaggcggc gcggagaagc ccaagatgga ggtcgcggtg caggacgatg ccgtcctgct
       61 ccagcgcgcc tactacgacc gcaacaaggc gctcgaccag gcgagggacc tcgaggtcac
      121 gcgcatccgc acgctcgtga tctggggccg cgtgccgggc tcgcagcccg acagcaccac
      181 gccgcccgcg ggaggcaggc ccacctacga cttctcggcc tgggactcgc tgatcgacga
      241 cgcggccaag cgcggcatcc gagtgcagct cgacctgacc ggccccgtgc cggcgtgggc
      301 cgccggcgac cgcaaggtcg gggtgacgcg gcccgacgcg cagaagttcg gcgtcttcgc
      361 cgagcaggcg gcccgccact tcaagggccg cgtcgaccgc tacagcatct ggaacgagcc
      421 gaactacggc agctggctga agccgcagac cgaggccgcg gatctgtacc ggaacctcta
      481 cctggcggcg tacaaaggga tcaagcgcgc ggatcccgcc gcgcatgtgc tgattggtga
      541 gacctcgccg tacgagctgg cggggcgcgc gatagctccg ctcaaattcc tgcgcgacgt
      601 gacctgccgc acgacgatct acgcgccggc caagcgctgc ccgacgctgg tggccgatgg
      661 ctacgcgcag catccctacg acttcgtgag cccgccggag cgcccgcatc cgggggccga
      721 caacgtcacc atcgggtcgc tggaccggct cacgagagcg ctcgacaagc tggcggaagg
      781 caaggcgctc acgacacccg ccgggaagcc cctcgacatg tacctcacgg agttcggcta
      841 cttcgcgagc ggcccggccg cgccgccgcc gaagcagcgc gcgagctggc tcgcgagggc
      901 attcgacatt gcctcgcgca acccgcgcgt gcgcgagatg ctccagtaca tcctggtgtc
      961 cccgccgaac aaggtgcgct tcgacaccgg ggtcatcacc ccgcaggggc agccgacggc
     1021 ggcgtatacg gcacttcgtg actgggcgca caaggctgcg gctgcgggca gcatcgacaa
     1081 gccggacggg ccgatcgagc tgcacacgcg accagcttcg taacaggacg tcggcgggcg
     1141 cgcctatatc ctcagaggag gaatggcctc cagaaggcag ataaacgtcg gcggcgtgcc
     1201 gatcggcggc ggtgcgcccg tggccgtgca gtcgatgacg aagactgaga ccgccgacta
     1261 cgacgccaca atgcggcaga tccgcgcgat cgccgacgcg ggcggcgacc tcgtgcgctg
     1321 cgccgtgccg cgcgaccagg acgtggaggc gctctcgcgc atcgtgaagg agtcgccgat
     1381 cccggtgatc gccgacatcc acttcaacta cacgcttgca ctgaaggcga tcgacgccgg
     1441 ggcgcactgc atccgcctca atccgggcaa catcggcggc cccgacaagg tggcgcaggt
     1501 ggtcgagaag gccaaggccg ccggcgtgcc gatgcgcatc ggcgtcaact ccggctcgtt
     1561 gcccaagcac ctgcacgcgc tagagcgcga gaactccgtg gaggccctcg tgaccgccgc
     1621 cgtcgagttc gtcgagctga tggagcggct cgagttcgag aacttcaagg tctcgatcaa
     1681 gtccacgagc gtcccgaaca cgatcgcctc gaaccggctg ctgtctgagc ggatcgacta
     1741 tccgctgcac ctcggcatca ccgaggccgg cacgaagtgg tcgggctcgc tcaagtccgc
     1801 cgttggcctc ggcacgctgc tggcggacgg gatcggcgac accatccgca tctcgctctc
     1861 caccttccac gcggaggagg aggtcaaggt cgcctgggag atcctcaagg cgctcaaact
     1921 gcgcgaaaaa ggcccggtgc tcatcgcctg tcccacctgc gggcgcctcc agttcgacat
     1981 ggacacggtg gtcgccgaga tcgagcgccg gctcgaggcc tacgacgacc cggtcgaggt
     2041 ggcggtgctc gggtgcgcgg taaacggcat cggcgaggca tcgcacgccg acttcggcat
     2101 caccggcgcc aagaacgagg ggctgatctt ctcgcgcggc aagccgctca agaaggtgcc
     2161 ccaggagcgg ctcgtggacg agctgttcgc cgagatcgac aagtacgtgg ccgcgggccg
     2221 caccgaggtg gacgccaaga aggcggccga gggggcggaa tggctcgagc gcatcgagga
     2281 ggagaacgcg ggcgagctca cgcccgagcg cctcgccaag ctcgaggccg agcaggcgcg
     2341 cgccgagaac ggcgagggca acggcgccgt cgagctgccg caggtcaaga aggtcgcgct
     2401 cgacgaggac gtctcgccga cggccggccg ccgcttcacc cgggcctgac cggccttctt
     2461 gctaggttgc gggtccgtcc cgcccccgga ccctctcttc atgctcaaga acacgaagcg
     2521 attcacattc gccgcgctgc tcgctgtgag cgcgctggcc acgaccgcgg gcgccgcgca
     2581 ggcgtcgaag acccaggagt cgatcttcca ggacgaccgc gtgctggtgt cctcgggccc
     2641 cggcgtgcag acgtccgcgc tcgatcagct ccaggcactc ggcgtcgaca cgatccactc
     2701 gcttgtcttc tggaacaaga tcgctccgag ctctgactcc tccaagaagc cgagcttcga
     2761 cgcgtcgaac ccggcggcct acccggcggg cacctgggac gcctacgacg acctcgtccg
     2821 cggcgccaag gcgcgcggca tcgacttgct cctgagcccg accgggaagt cgcccagctg
     2881 ggcgaacggc tgccgtggca aggcgaactg ccagccgagc gcgaaggagt tcggcgcctt
     2941 tgtgaaggcc gtcgccaagc gctactcggg cacctaccac gacgagaacc agggcggcgg
     3001 cacgctcccg aaggtgggtc gctggtcgat ctggaacgag cccgaccagg gtggctggat
     3061 cttcccgcag cgtaagagcg gggtggtgta ctcggcgaag atctaccggg acctctacac
     3121 ggcgggctat agcgcgctga aggcgacggg ccatggccgc gacaagatct acatcgcgga
     3181 gaccgcgccg gtgcgcgggg gagtggcgac cgcgccggtc gagttcctgc aggcgctgtt
     3241 ctgcatcgac aagaacggcc gcaagctcag gggcaaggcg gcgaagacgc tcggctgcac
     3301 gaagtacaag acgctcaagt tcactgcggt ggcgcaccac ccgtacaacc gcggcgccgg
     3361 cgggccgccg ctcaagaagc cgaagaccgc cggtgacatc acgctggcga ccctcgaccg
     3421 tctcttcacg gtgctcaagc aggcgaagag ggcgcaccgc atcagcggct cgacctcgag
     3481 cgtctacttc accgagtacg gcatccagtc gaggccgccc gagcagaagt tcggcgtgcc
     3541 gctcacgcag catgcggccc agctgaacca ggccgagtac gtgggctaca acgaccggcg
     3601 cgtgaagagc ttctcgcagt acgagctctt cgacgagccg gatgtgggca cgttcaacac
     3661 cggcctgctg ttcacgaacg gcaccgcgaa gccggcgctg gccgcctacc gcctgccgat
     3721 ctatgccgtg aagagcggca gcaaagtgaa gctctggggc gccgtccgcc ccggcaacgg
     3781 cagccgcagc gtggacgtcc tcgcgggcgg caaaaaggtg aagaccgtca ccgtccgcgg
     3841 agccggctac ttcaccacca ccgtgagcaa ggcctcgagc tatcagctgt cggccacgat
     3901 cggcggccag acgttcaaga gccgcgtggc caaggccgcg tcgcgctgat ccacgcatcg
     3961 ggcgtccgaa tagactcggg cgcccgatga cgaggctctc caactacctg ctgccgacgg
     4021 tcaaggaggc gcctgccgag gcggaggcga tctcgcacaa gctgctggtg cgagccggcc
     4081 tggcgcgcca gctcagctcg ggcctgtgga cgtacctccc ggccggctgg cgcgtgcacc
     4141 gcaaggtcga gcagatcatc cgcgtggaga tcgacgccat cggcggccag gagatgctga
     4201 tgccggtgct ccagccggcg gacatatgga agacgagcag ccgctactcg atcgaagagc
     4261 tgttcaagct gaaggaccgg aagggcgccg atctcgtgct cgcgatgacc cacgaggagg
     4321 cgctcacgtg gcacatggcg cgcgagatcc gctcctaccg cgacctgccg
//
LOCUS       DASVXS010000269         6544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_891762, whole genome shotgun sequence.
ACCESSION   DASVXS010000269 DASVXS010000000
VERSION     DASVXS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891762"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VF752_15200"
     CDS             <1..1006
                     /locus_tag="VF752_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX8856940.1"
                     /translation="GASWSGHIGIRSIKIKKGLLYINGVRTTLRGASIHEDDPNVGAA
                     LTPQIRQSMFGELLRLGGTLTRSHYPLHPDFLEMADRAGVLVWQQIPFYRLGEDQMKL
                     KSVRDKGLNMLRAEISRDQNHPSVMTWSIGNELPSRPAFGQERYLSDSTRLLHQLDPT
                     RLVALDISGYPSAPVVGQYNQLDALGINDYFGWYPGPNGQLVDRNALGPYLDQMRGYY
                     PKLGLFVTEFGAEANRNGAVDEKGTYQFQSDWMQFQIATIDSKPYMSGAINWLLRDFK
                     VRPDWDGGNPIPQPPYNQKGVSDQFGSPKPSFDVTARMYKAIKRAGAARIARASEAAR
                     "
     gene            complement(1003..2211)
                     /locus_tag="VF752_15205"
     CDS             complement(1003..2211)
                     /locus_tag="VF752_15205"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8856941.1"
                     /translation="MLLPRLVLACVLAAGLLTGGCGSGKSNKQTVTSAPNPAPAPTST
                     DSRNAAPSRRGVRLVRVGNFDQPVYVTSPPGDTSRLFVVEQAGRIRVVRNGRKLSQPF
                     LDIRSQVDCCGERGLLSMAFPLNYATSKLFYVYFTDKAGDIRVVQFRASSSDRADAVS
                     ARQILFQEHSEFANHNGGQLQFGPDGDLYLGLGDGGSEGDPNNHAQDLGSQLGKLLRV
                     RPRTSGGHTVPRDNPFVGRAGAAPEVYAYGLRNPWRFSFDRRTGDLVIGDVGQSEIEE
                     IDFARRGRAKGMNYGWSRFEGTRRNSDRTAPGARRPVLQHSHAQGFCSIIGGYVVRDR
                     SLSLYGRYVYGDLCDPDIWSVKLISSVRASADRRTGLRVPNLSSFGEDAHGHVYLASL
                     AGPVYRLASR"
     gene            complement(2238..2489)
                     /locus_tag="VF752_15210"
     CDS             complement(2238..2489)
                     /locus_tag="VF752_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856942.1"
                     /translation="MAEREELGEEQFLESLMDTNLYSMGAYFSDQHPDLVDEVIDQAI
                     AIEQVGLRRYAHEHGITLEACFETLLTGLAVRYYKAVAA"
     gene            2531..3289
                     /locus_tag="VF752_15215"
     CDS             2531..3289
                     /locus_tag="VF752_15215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX8856943.1"
                     /translation="MAGRDEIIDFLDDLLDSPAFDDYGPNGLQVPGAEEVRLVVSGVS
                     AHRELFEAARDAGAQMVLCHHGIFWRGAPLQVTPQSKERLKVLFDSDISLAAYHLPLD
                     AHPEVGNNALICRALGFERQRSFDEHGGRDIGSIARNDTGLPAAKLFARCAELFGQEP
                     LVFDAGADAVHTIGVVSGGGSRAISEAAALGADAFITGEPSEFVMADARELGVHYIAG
                     GHWATETFGVRRLGELLAERFGVEHRFIAVPNPI"
     gene            3429..3605
                     /locus_tag="VF752_15220"
     CDS             3429..3605
                     /locus_tag="VF752_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856944.1"
                     /translation="MAIHLTPTELAREAGMQRREVISKCMEFGVPIFQGRIDKTLFMS
                     SLKDAQRKQQLAKV"
     gene            3708..5498
                     /locus_tag="VF752_15225"
     CDS             3708..5498
                     /locus_tag="VF752_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEX8856945.1"
                     /translation="MEAGTVTAAEASTGSKTMADLIVLAAQKHGDRTAIKHKVGDQWV
                     DVSYGQMGETVKQVALGLIELGIEPGDKVSILSNTRPEWTYAQFGILAAGATTVSIYQ
                     TNSPQECHYVLSHSESRAVFVEDLEQLDKIRQIRNELPKLEFVIVFDPPAGGASDAIA
                     LSTLMERSAEADESQFSERVNGVSRDDVCLYIYTSGTTGPPKGCILTHGNYRDVTTMT
                     ESQGVLTGDELVYLFLPLAHAFALLIQFVTFDLGSAIAYWEKDPQKIVPNLMELKPTY
                     FPSVPRIFEKIYTLATSNAPDRDALDRAVKLGFKVRLADVRGEEVSPEERAAFEKADQ
                     ELFVNVRNLFGGRIRQAVTGAAPIAREILEFFYACGVPVMEGYGMTETSTVASANSID
                     DFRFGSVGRPLPGVEAKVAEDGELLLKGSNIFKGYFKNEQATKETLEDGWLHTGDLAT
                     IDEDGYIYITGRKKDIIITAGGKNITPANLENGLKQNRWISQAVVVGDRRPYLVALIT
                     LDPEEVPAFAEMHGLEPAQVRDSEQMRAEIQKTVDKVNDEVGRVEQIKKFTILPHDLS
                     QETGELTPTLKVKRNVVAEKCAGDIESLYA"
     gene            5586..6062
                     /locus_tag="VF752_15230"
     CDS             5586..6062
                     /locus_tag="VF752_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856946.1"
                     /translation="MPRNDPTDTGGLFIGRRPGTAPLRYRGTPVRAGGFRRRFDSALA
                     TAILVVETLLCATLWGPQPLGWLWVGSQVDYQTGSVTLGILSAFAGMFTTLFLTLALA
                     KRLDHVWQLVRRAAGHDQRKGVLDRIFVVSIAIAGTGFLFWFLIIAGPGPTLAPRQ"
     gene            6075..>6544
                     /locus_tag="VF752_15235"
     CDS             6075..>6544
                     /locus_tag="VF752_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8856947.1"
                     /translation="MGLLDYYRQFEDMDEEEVNRGLRERRARERAQQLQVVEKIDLSS
                     TEWPDFPNSEVVNAAIAMARGRVNGYPDRHATKVRAELAARHGVEPEQVVIGNGAAEL
                     LQAAALTLLAPGDELVTAWPSYPLYPLMASRAGARPVAVDLAGGRAQVDALIAA"
BASE COUNT         1108 a         2208 c         2205 g         1023 t
ORIGIN      
        1 cggcgcgagc tggtcgggcc acatcgggat ccgctcaatc aagatcaaga agggactcct
       61 ctacatcaac ggcgtgcgca cgacgctgcg cggcgcctcg atccacgagg acgacccgaa
      121 cgtgggcgcc gctctcaccc cgcagatccg gcagtcgatg ttcggcgagc tgctgcgcct
      181 cggcgggacg ctgacgcgct cccactaccc gctccatccc gacttcctcg agatggcgga
      241 ccgcgcgggc gtgctcgtgt ggcagcagat cccgttctac cggctcggtg aggaccagat
      301 gaagctgaag tcggtgcgcg acaaggggct gaacatgctc cgcgccgaga tctcacgtga
      361 ccagaaccac ccttcggtca tgacatggtc gatcggcaac gagctgccgt cgcgacccgc
      421 gttcggccag gagcgctacc tcagcgactc gacccggttg ctgcatcagc tcgacccgac
      481 gcggctggtg gcgctcgaca tctcgggcta cccgtcggcg ccggttgtgg gtcagtacaa
      541 ccagctcgac gctctcggca tcaacgacta cttcggctgg tatccgggcc cgaacggcca
      601 gctcgtggac cgtaacgcgc tcggcccgta tctcgaccag atgcgcggct actacccgaa
      661 gctgggcctg ttcgtgaccg agttcggtgc ggaggcaaac cgcaacggcg ccgtcgacga
      721 gaagggcacg taccagttcc agtccgactg gatgcagttc cagatcgcga ccatcgactc
      781 caagccctac atgagcggtg cgatcaactg gctgctacgc gacttcaagg tgcggcccga
      841 ctgggacggg ggcaacccga tcccgcagcc gccctacaac cagaagggcg tctccgatca
      901 gttcggcagt ccgaagccgt cgttcgacgt gacggcgcgc atgtacaagg ccatcaagcg
      961 tgcgggcgcg gcgcggatcg cgcgcgcttc cgaggcagct cgctagcgcg aagccaaccg
     1021 gtagaccggg cctgcgagtg acgccaggta cacgtgcccg tgcgcgtcct cgccgaacga
     1081 gctgaggttc ggtacccgca ggccagttcg gcggtccgcg ctcgccctga cgctggagat
     1141 gagcttcacg gaccagatgt ccgggtcaca gaggtcgccg tacacgtagc ggccgtagag
     1201 cgacagcgag cggtcgcgga cgacgtagcc gccgatgatc gagcagaagc cctgggcatg
     1261 tgagtgctgg aggaccggtc gcctcgcgcc tggcgctgtg cggtccgagt tgcggcgcgt
     1321 gccctcgaag cggctccagc cgtagttcat gcccttcgcc cggcccctgc gagcgaagtc
     1381 gatctcctcg atctcgctct gccccacgtc accgatcacg agatcgccgg tgcgacggtc
     1441 gaacgagaac cgccacgggt tgcggaggcc gtacgcgtat acctccggcg ccgccccggc
     1501 gcggccgacg aacgggttgt ccctcgggac ggtgtggccg ccactcgtcc gcggcctgac
     1561 gcggagcagc ttgccgagct ggctgccgag atcctgcgcg tggttgttgg ggtcgccctc
     1621 gctgcctccg tcgccgagcc cgagatacag gtccccgtcg gggccgaact ggagctgccc
     1681 gccgttgtgg ttggcgaact cgctgtgttc ctggaacagg atctgcctgg cgctgaccgc
     1741 gtcggcccgg tcggatgatg aggcgcggaa ctgcaccacg cggatgtccc cggctttgtc
     1801 ggtgaagtag acgtagaaca gcttcgacgt cgcgtagttc agcgggaacg ccatcgagag
     1861 caggccacgc tcgccgcagc agtcgacttg cgaacggatg tcgaggaatg gctggctcag
     1921 cttgcggccg tttcgcacca cccggattcg ccccgcctgc tccaccacga acagccggct
     1981 cgtgtcaccg ggcggcgagg tcacgtacac gggctggtcg aagttcccca cgcgcacgag
     2041 gcgcacccca cgccgcgacg gtgcggcgtt gcgcgagtcc gtcgaggtgg gcgcgggcgc
     2101 cgggttcggc gcgctggtga cggtctgctt gttgctcttg cccgacccgc atccgcccgt
     2161 gagcaggccc gccgcgagta cgcaggcgag tactagtcgg gggaggagca tcgaggaacg
     2221 gacgatggca ggcggcctta agccgcgacc gccttgtagt accgcaccgc gagaccggtg
     2281 aggagcgtct cgaagcaagc ttcgagcgtg atcccgtgct cgtgcgcgta cctgcgcaga
     2341 ccaacttgct cgatggcgat ggcctggtcg atcacctcgt cgacgaggtc cggatgctgg
     2401 tcggagaagt acgcgcccat ggagtacagg ttcgtgtcca tgagcgattc gaggaactgc
     2461 tcctcgccca gttcctcgcg ctccgccatg gcgccgcaga ttacctgggc gcggtaggtt
     2521 cacgcaccca atggccgggc gcgacgagat catcgacttc ctcgacgacc tgctcgactc
     2581 ccccgcgttc gatgactatg ggccgaacgg actgcaggtg ccaggggccg aggaggtgcg
     2641 gctggtggtg agcggcgtct cggcacaccg cgagctgttc gaggccgccc gcgacgcggg
     2701 cgcccaaatg gtgctctgcc accacgggat cttctggcgg ggagccccgc tccaagtgac
     2761 gccgcagtcg aaggaacgac tcaaggtgct gttcgactcg gacatttcgc tcgccgccta
     2821 tcacttgccg ctcgacgcgc atcccgaggt ggggaacaac gcgctcatct gccgggcgct
     2881 cggcttcgag cgccagcgat cattcgacga gcacggcggc cgcgacatcg gctccatcgc
     2941 gcgcaacgac accggcctac ctgccgccaa gctgttcgcg cgctgcgccg agctgttcgg
     3001 gcaggagccg ctcgtgttcg acgctggtgc agacgcggtg cacacgatcg gagtcgtgtc
     3061 gggcggcggc tcgcgcgcca tctccgaggc ggccgcgctc ggggccgacg cattcatcac
     3121 cggcgagccg agcgagttcg tgatggcgga tgcgcgcgag ctcggcgtgc actacatcgc
     3181 cggcgggcac tgggccacgg agacgttcgg cgtgcgccgc ttgggcgagc tgcttgcgga
     3241 gcgcttcggc gtcgagcatc gcttcatcgc ggtgcccaac cccatctgag gtacttcccg
     3301 ggcgatatcg ggccggatcg gcctcaattc cgccgcaaat tgggcgcttc ggtttgcgac
     3361 gtctgacgca agctgtaaat tcctttgtcc aagcagtacc gaacataaga gccgacggga
     3421 ggagccgcat ggcgatccat ctcacgccca ccgaactcgc acgcgaggca ggcatgcagc
     3481 ggagagaagt catctcgaaa tgcatggagt tcggtgtgcc gatctttcag ggacggatcg
     3541 acaagacgct tttcatgtcc tcgttgaagg acgcgcagcg caagcagcag ctcgccaagg
     3601 tctaactccg gcgggctctt tcattttggt ggcgcctgcg tcacttttct cgcgctaggc
     3661 gcgacagccg catagactcc accttccgag cgactcgagg agggtggatg gaagcaggga
     3721 cggttaccgc cgcggaggcc agcaccggct ccaagacgat ggctgacctg atcgtgctcg
     3781 cggcccagaa gcacggcgac cgcaccgcga tcaagcacaa ggtgggcgac cagtgggtcg
     3841 acgtcagcta cgggcagatg ggcgagaccg tcaagcaggt ggcgctcggc ctgatcgagc
     3901 tgggcatcga gcccggcgac aaggtctcga tcctctccaa cacccgtccc gagtggacct
     3961 acgcgcagtt cggcatcctg gcggccggtg ccacgacggt gtcgatctac cagacgaact
     4021 cgccgcagga gtgtcactac gtcctgagcc actcggagtc gcgcgccgtg ttcgtcgaag
     4081 acctcgagca gctcgacaag atccgccaga tacgcaatga gctgccgaag ctcgagttcg
     4141 taatcgtgtt cgacccgccc gcgggcggag cgagcgacgc catcgcactg agcacgttga
     4201 tggagcgcag cgccgaggcc gacgagtcgc agttcagcga gcgcgtcaac ggcgtctcgc
     4261 gcgacgacgt gtgcctctac atctacacct cgggcaccac cggaccgccc aagggctgca
     4321 tcctcacgca cggcaactac cgcgacgtga cgacgatgac ggagagccag ggtgtgctca
     4381 cgggcgacga gctcgtctac ctcttcctgc cgctcgcgca cgcgttcgcg ctgctcatcc
     4441 agttcgtcac cttcgacctc ggctcggcga tcgcgtactg ggagaaggac ccccagaaga
     4501 tcgtgcccaa cctgatggag ctgaagccga cctacttccc gtcggtgccg cgcatcttcg
     4561 agaagatcta cacgctcgcc acgagcaacg cacccgaccg cgacgcgctc gaccgcgcgg
     4621 tcaagctcgg cttcaaggtg cgcctcgccg acgtccgagg cgaggaggtg tcgccggagg
     4681 agcgcgccgc gttcgagaag gctgaccagg agctgttcgt gaacgtgcgc aacctgttcg
     4741 gcggccgcat caggcaagcg gtgaccggag cggccccgat agcgcgcgag atcctcgagt
     4801 tcttctacgc gtgcggcgtg cccgtgatgg agggctacgg gatgaccgag acgtccacgg
     4861 tcgcgagcgc gaactcgatc gacgacttcc gcttcggctc ggtcggccgg ccgctgccgg
     4921 gcgtcgaggc gaaggtcgcc gaggacggcg agctcctgct gaagggctcg aacatcttca
     4981 agggctactt caagaacgag caggccacga aggagacgct cgaggacggc tggctgcaca
     5041 cgggcgacct cgcgacgatc gacgaggacg gctacatcta catcaccggc cgcaagaagg
     5101 acatcatcat caccgcgggc ggcaagaaca tcacgcccgc caacctcgag aacggtctga
     5161 agcagaaccg ctggatctcg caggctgtgg tggtcggcga ccggcgaccg tacctcgtcg
     5221 cgctgatcac gctcgacccc gaagaggttc ccgccttcgc cgagatgcac gggctcgagc
     5281 cggcgcaggt gcgcgactcg gagcagatgc gcgcggagat ccagaagacc gtggacaagg
     5341 tcaacgacga ggtcggccgc gtcgagcaga tcaagaagtt cacgatcctc ccgcacgacc
     5401 tgtcgcagga gaccggcgag ctgactccca cgctgaaggt gaagcggaac gtggtcgccg
     5461 agaagtgcgc cggcgacatc gaaagcctct acgcctgagg attcagccac ccgcctcgaa
     5521 ggctgagccg ggtggctacc atcggtgtgc gggacgcgcc ttgacccctg cccccgctcg
     5581 acaggatgcc gaggaacgac ccgacagaca cgggcggcct cttcatcggc cgccgtcctg
     5641 gcaccgcacc gctccgctac cgcggaacgc cggtgcgagc gggtggcttc cggcgccgct
     5701 tcgacagcgc tctggccacg gcgatcctgg tcgtggaaac gctcttgtgc gcgacgctgt
     5761 ggggtccgca gccgctgggg tggctctggg tgggttcgca ggtggactat cagaccgggt
     5821 cggtgacgct cggcatcctg agcgccttcg ccgggatgtt caccacgctg ttcctcacgc
     5881 tcgcgctcgc aaagcggctc gaccacgtgt ggcagctcgt gcgccgcgcg gccgggcatg
     5941 accagcgcaa gggagtgctc gaccgcatat tcgtcgtgag catcgcgatc gcgggcaccg
     6001 gcttcctctt ctggttcctg atcatcgccg ggccggggcc gacgctcgcc ccgcgtcagt
     6061 agcctgaggc agccatgggg ctgctcgact actacaggca gttcgaggac atggacgagg
     6121 aggaggtcaa caggggcctc cgcgagcgcc gcgcgcgcga gcgcgcccag caactgcagg
     6181 tggtcgagaa gatcgacctc tccagcaccg agtggccgga cttccccaac tcagaggtgg
     6241 tgaacgccgc gatcgccatg gcgcgcgggc gtgtgaacgg ctatcccgac cgccacgcca
     6301 ccaaggtgcg tgccgagctc gcggcgcgcc atggcgtgga gccggagcag gtcgtgatcg
     6361 gcaacggtgc cgccgagctc ctgcaggccg cggcgctcac actcctcgcg cccggcgacg
     6421 agctggtgac cgcctggccg tcgtacccgc tgtacccgct catggcctcg cgcgccgggg
     6481 cccggcccgt ggccgtcgac ctcgcgggcg gtcgcgccca ggtggacgcg ctgatcgccg
     6541 cctg
//
LOCUS       DASVXS010000270         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1251633, whole genome shotgun sequence.
ACCESSION   DASVXS010000270 DASVXS010000000
VERSION     DASVXS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251633"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VF752_15240"
     CDS             complement(<1..1223)
                     /locus_tag="VF752_15240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX8856948.1"
                     /translation="MEVEIGRGKKGRRAYGFDDIAIVPSRRTRDPDDIDITWTLGPYR
                     FELPLLASAMDGVVSPTTAGILGKLGGLAVLNLEGIFTRYEDADEQLERIAQLPREHA
                     TAEMQRIYQEPVEPELIAQRIREIKEQGVVCAASLTPQKVRQYYEIALDAGLDILVIQ
                     GTVISAEHVSTTSEPLNLKEFIREVPVPVVVGGCASYSTGLHLMRTGAVGVLVGAGPG
                     AACTTRGVLGIGVPQATAIADVAAARSQHMLETGDYVKVIADGGMRNGGDISKAFACG
                     ADAVMIGSPLARAYEAPGRGYHWGMATFHPSLPRGARVNTAQNGTLEQIVLGPAHEND
                     GTFNLMGSLRTSMATCGYEDIPDFHRAEVMVAPALQTEGKQLQRSQEVGMGATAAAAA
                     APMDNGSAGELSNGVA"
     gene            complement(1351..2145)
                     /locus_tag="VF752_15245"
     CDS             complement(1351..2145)
                     /locus_tag="VF752_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX8856949.1"
                     /translation="MTVVSPPPLRSAPIATAAKEREHSLFLEYQSTGDMAAREELVER
                     FLPLARRLARKYERMNEPFEDLLQVASVGLVKAIDRYEPGQGTAFASYAVPTILGEIK
                     RYFRDSGWAVHVPRGVQERVLRVNQAIEKLSPELGRSPTPAEVSAELGDDLEDVLEAM
                     EAGGAYNTISLEAPLGTEDPDSGTYADSVGDLDDRYDRVEMSDTLSRAMKAVPERERM
                     ILFLRFNEGLTQSEIAERLGISQMHVSRLIRRTLDRLRVVASARAA"
     gene            complement(2382..>2775)
                     /locus_tag="VF752_15250"
     CDS             complement(2382..>2775)
                     /locus_tag="VF752_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8856950.1"
                     /translation="DRLRAVTEIAELGPREAVMTLASGCIGLLPADGHDGTAPTTGAP
                     GRLYQVMLDPLEPVSAVGSGDAFLAGYVAARYTGREPEACLRFAVACGAESTQHFGAG
                     VLDPREVDRLLSEVRVEQVEMPALEARR"
BASE COUNT          406 a          978 c          953 g          438 t
ORIGIN      
        1 gcaacgccgt tcgagagctc gccggcggag ccgttgtcca tcggagccgc tgcggcggcg
       61 gcggtggcgc ccatgccgac ctcctggctg cgctgaagct gcttgccctc ggtctgcagc
      121 gccggcgcga ccatcacctc ggcgcgatgg aagtccggga tgtcctcgta cccgcaggtg
      181 gccatcgatg tccgcaggct gcccatcagg ttgaaggtgc cgtcgttctc gtgcgccggc
      241 ccgagcacga tctgctcgag cgtgccgttc tgggcggtgt tgacgcgcgc gccgcgcggc
      301 agcgagggat ggaatgtggc catgccccag tggtagccgc ggcccggagc ctcgtaggcg
      361 cgggcaaggg gcgagccgat catcacggcg tcggcgccgc aggcgaacgc cttcgagatg
      421 tcgccgccgt tgcgcatgcc gccgtccgcg atcaccttca cgtagtcgcc ggtctcgagc
      481 atgtgctgcg agcgcgcggc cgcgacgtcc gcgatcgcgg tcgcctgggg gacgccgatc
      541 ccgagcacgc cgcgcgtggt gcaggcggcg cccggtcccg cgccgacgag cacgcccacc
      601 gcgccggtgc gcatgaggtg caggccggtc gagtacgagg cacagccgcc gacgaccacc
      661 ggtacaggga cctcccggat gaactccttc aggttgagcg gctcgctcgt ggtcgagacg
      721 tgctccgccg agatgaccgt gccctggatg acgaggatgt cgagccctgc gtcgagagcg
      781 atctcgtagt actgccggac cttctgcggg gtgagcgatg ccgcgcacac cacgccctgc
      841 tccttgatct cgcggatgcg ctgcgcgatc agctccggtt cgaccggctc ctggtagatc
      901 cgctgcatct ccgccgtggc gtgctcgcgg ggcagctgcg cgatccgctc cagctgctcg
      961 tcggcgtcct cgtaccgcgt gaagattccc tcgaggttca gtaccgcgag cccgccgagc
     1021 ttgccgagga tgccggccgt ggtcggagac accacgccgt ccattgctga ggcgagcagc
     1081 ggcagctcga agcggtacgg cccgagcgtc caggtgatgt cgatgtcgtc agggtcgcgc
     1141 gtgcgccgtg acggtacgat cgcgatgtcg tcgaagccgt acgcgcgacg accctttttc
     1201 ccacgtccaa tctctacttc catggcggca tcctatgttc ggcggcggac cgaaaggacg
     1261 ataccaacgc cttttcggac ctccgagaac gccgctttct cgcaagctgc gggaacccat
     1321 gcgcgccgat ttggcgcgcc cgcacaatgc tcaggcagcc cgcgccgaag cgaccacccg
     1381 caggcggtcg agcgtccggc ggatgagccg cgacacgtgc atctgggaga tgccgaggcg
     1441 ctccgcgatc tccgactgcg tgagaccttc gttgaagcgc aagaacagga tcatgcgctc
     1501 gcgctcgggg accgccttca tcgcccgcga cagcgtgtcg ctcatctcga cgcggtcgta
     1561 gcggtcgtcg aggtcgccga ccgagtctgc gtaggtgccg ctgtccgggt cctccgtgcc
     1621 gaggggcgcc tcgagcgaga tcgtgttgta ggcgccgccg gcctccatcg cctcgagcac
     1681 gtcctcgagg tcgtcgccca gctcggcgga gacctcggcc ggcgtgggtg agcggccgag
     1741 ctccggagag agcttctcga tcgcctggtt cacgcgcagc actcgctcct gcacgccgcg
     1801 cggcacgtgc accgcccaac ccgagtcgcg gaagtaccgc ttgatctcgc cgaggatcgt
     1861 cgggaccgcg tagctcgcga aggctgtccc ctgaccgggc tcgtagcggt cgatggcctt
     1921 cacgagcccc acgctcgcga cttggagcag atcctcgaac ggctcgttca tccgctcgta
     1981 cttgcgcgcg aggcgccggg caagcgggag gaagcgctcg acgagctcct cgcgggccgc
     2041 catgtcgccc gtggactggt attcgaggaa cagggaatgc tcgcgctcct tggccgcggt
     2101 ggcgatcgga gcggatctga ggggtggggg agaaacgacc gtcatgggaa actgcctcct
     2161 tccgcactga tggcttcacc acacgaagat ccggcggcgg cccaatgcgg accacggtcg
     2221 tctcgccgac gcgaaacact ctcactctgt tggcttcggg tccagccgct tcgctccgga
     2281 cgccgacgcc tatatcaagc gttcccgtaa gccggttccc caaacaagcg ggagggacgc
     2341 ttctgtccga acgctgactt ggcgcgcgcc cgcgcgcccg atcagcgccg cgcttcgagc
     2401 gcgggcatct cgacctgctc cacccgcact tcggaaagca acctgtcgac ctcgcgcggg
     2461 tcgagcactc ccgcgccgaa gtgctgcgtg gactcggctc cgcaggcgac ggcgaaccgc
     2521 aggcacgcct caggctcgcg gccggtgtag cgagcggcca cgtagcccgc caggaacgcg
     2581 tcgcccgagc ccacagccga gaccggctcg agcggatcga gcatgacctg gtagaggcgg
     2641 cccggggcgc ccgtcgtagg cgcggtgccg tcgtgcccat cggcgggcag caggccgatg
     2701 catcccgacg ccagcgtcat cacggcctcg cgtgggccca gttcggcgat ctccgtcacg
     2761 gcgcgcagcc ggtcc
//
LOCUS       DASVXS010000271        27072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1104382, whole genome shotgun sequence.
ACCESSION   DASVXS010000271 DASVXS010000000
VERSION     DASVXS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 27072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27072
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104382"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VF752_15255"
     CDS             <1..541
                     /locus_tag="VF752_15255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856951.1"
                     /translation="FYPANIWPHKNHERLVDALTRVEDRELALVLSGQDHGKLPALLE
                     RARAAGVGDRVMHAGYVERAQLPAIYRRATGVIFPSLFEGLGIPPLEAQACGCPVAAA
                     RVASLPEVLGERALWFDPRDTDAIAGAIDALAAGRAPGGTPPESFWSRFTWEAAAADH
                     RRAYALAAGQRSGTRMAVR"
     gene            538..1554
                     /locus_tag="VF752_15260"
     CDS             538..1554
                     /locus_tag="VF752_15260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX8856952.1"
                     /translation="MSDLSGRRVLVTGGAGFLGSHVVNLLRERGAELIVPRSAEFDLV
                     VEDDVERLIAEARPEMVMHLAARVGGIGANQRNPGAYFYANLMMGALLMEHSRRAGVE
                     KFLQLGTICAYPKFTPVPFHEDDLWNGYPEETNAPYGIAKKALLVQAQSYREQYGFNA
                     VYLLPVNLYGPKDNFDRESSHVIPALIRKMIEAEENGDGSVVLWGDGSPSREFLYVDD
                     CAEALVLALERYDKPDPVNIGAGFEITIRDLAELVREITGFDGEIEWDTSKPGGQPRR
                     MLDTSRAEREFGFKAMTGFREGLQKTVDWFRKNRDWVAEDERRRKESERRAMAAAGLL
                     TGSP"
     gene            1650..2399
                     /locus_tag="VF752_15265"
     CDS             1650..2399
                     /locus_tag="VF752_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8856953.1"
                     /translation="MRKSYLAIIPALNEEHTIASVIDGVRLHAPQFDVVVIDDGSSDR
                     TREVSEAAGAPVIRHPFNLGIGGAVQSGFSYAQENGYDFMVQVDGDGQHDPAEVAKLI
                     DRMAQNGVDMVCGSRFMEKTGYAAPRARRTGIRIFSAILSRIVGQQVTDPTSGFRLFN
                     RRAIGLFARDYPHDYPEVEAVLMLHFHQLRMCEVPVTMYQRGGGVSSISTGKSAYYMI
                     KVLMAIVVGLVRARPVVEPGDEPPVAAAHGI"
     gene            2399..2812
                     /locus_tag="VF752_15270"
     CDS             2399..2812
                     /locus_tag="VF752_15270"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX8856954.1"
                     /translation="MGTRIQIVSIVATVALFVIVFELVRRRRLQERYALLWLFAAVVL
                     FGLAAWQGLLSKIASAVGIFYPPSALFVIAFGFILVLLLHFSVAVSRLSDQTKVLAQR
                     LALTEKRLRDLEAGAEPEVREEERAPETVGRAPTP"
     gene            complement(2802..4364)
                     /locus_tag="VF752_15275"
     CDS             complement(2802..4364)
                     /locus_tag="VF752_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856955.1"
                     /translation="MTVPPRETGVVPADEVPRAAAPTTGPPASDDARHTAIISLGQAS
                     AMVMGGVLALLIAHIFGKSGRTDAFFAAYGVATVLIIFGQTFRITALPKLTDAGDPAA
                     TARLLAAVGAIVLVAAVPMVLLAGPVASVLVRSDPGHVAAHSLRLLWIAVAGQLFGSM
                     LAAILATRGRFVLIGVSYLVTGLVSVAVFLALESSVGIEAVAIGLDVAGVMLAVILGW
                     AFLQESGGRPAASPGGAGSIAREVRDLSLASATFVALNLGYVICVAVASRAGSGEATL
                     YAYAYFSAAILVAMTATSAAMVRSPRLLSSEARSEQVMAQSAMATYRVTVVVVTPVLA
                     VAALVGQPVLEFVLGGAFGHDDTVRLVETLLLLSGWVLGTAAGTFAVVELLARARLRE
                     LALIGVAQVLLLVPLAIAGREAWGIRGIALAQSLVVLAATAAQLHLAFAADARRVVRH
                     MAADTGRAVGAMAVAFAPSVALVLALGYDAAVVVPAAALAVALAVVATRVAWPDEWRL
                     LTSVVRRRAATA"
     gene            complement(4361..5152)
                     /locus_tag="VF752_15280"
     CDS             complement(4361..5152)
                     /locus_tag="VF752_15280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8856956.1"
                     /translation="MDVPSLSIVTPCLNAAGTLETALESVRSQGYPKLEHIVVDGGSN
                     DGTVEILERAEGVRFISEPDRGLSDAMNKGVRMATGELVGWLNADDVYLPGALRRVGE
                     EFARHPEAMWGFGRCPIIDGNGNEIRTAVTAYKDFFLRRWSFPLHLVQNFVSAPGTFV
                     RREAFDRVGMFDERFRYSMDYDFWLRLGRQGWRPLFIDEEIAAFRMAPGSLSMSGFET
                     QFVEHAENAREHGAGHPVAVGANVVISRLIPAVYRGMRYLRERGA"
     gene            5180..6199
                     /gene="gmd"
                     /locus_tag="VF752_15285"
     CDS             5180..6199
                     /gene="gmd"
                     /locus_tag="VF752_15285"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX8856957.1"
                     /translation="MAGKKALITGITGQDGSYLAELLLDKGYEVHGIVRRSSSFNTDR
                     IDHLYRDPHEQGVRLFNHYGDLADSVALTKLLYELQPDEIYHLGAQSHVRVSFDIPEY
                     TADIVGLGTIRMLEAIREAEVKTRFYQASSSEMYGKVQEVPQTETTPFYPRSPYAVAK
                     VAAFWATVNYRESYGMHASNGILHNHESPRRGETFVTRKITRAVARIKHGLQDKLYMG
                     NLDAKRDWGYAKDYVEAMWLMLQQDEPDDYVIATGQTHSVREFLEEAFGYADLDYRDY
                     VEIDARYFRPAEVDLLLGDPTKAKDKLGWEPRVTFKELVKLMVESDMEEVRAQLEGGA
                     AAVRR"
     gene            6306..7775
                     /locus_tag="VF752_15290"
     CDS             6306..7775
                     /locus_tag="VF752_15290"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEX8856958.1"
                     /translation="MPAPLALLLAVVALFCLAWVLIVPAGQVPDEEMHLQYALTLAER
                     GELPGHGPNGFSTQQWTAEVATNWERMPGAPERKPPWTNGEYEKWKAADAALPPGSKS
                     NGGGYIWQGDNPPLYYGYEAAAYHASPGRGFFDRLYFMRVMSALWVLVTATGAWLLAG
                     EIFGRDRPLQLVAAGFAGLLPMVDFVSSGVNPDAMLYAFWSVAFWLGARILRRGLTVR
                     DGVALCALVGVAFAEKSTSIALIPPMLLALAVGWRRLSRTRPQARRVATVAVLVSLAA
                     FAVPAGAWVVTAKALDRPITNRAPHYVGKPAPQLTSIQTDRQFLSYVWQFYLPRLPFM
                     QPIPLLGSGGDKLYRVWVSQVWGNFGWQEVNFPPWAYKGFAALSVLLLAGGAAAALRA
                     RRRGHFDLAIAAYFALAIVSLLFLLHWAEYTIMSLEGIAFNQGRYLLPLVALGGVVVA
                     ACVSLLRPSLRTPATGVVLGSLVVLQLFSLGLVVERYFV"
     gene            7768..8427
                     /locus_tag="VF752_15295"
     CDS             7768..8427
                     /locus_tag="VF752_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856959.1"
                     /translation="MSRRLLVPGVVVAVVAAGVLALVLVAAGNEQHIAYPLGFGDEGV
                     SAQIAPGAHACQQGAAHVPTSADGVRLTLGQPGSTPPVRVTVFGADRRVLGRGDIRGG
                     YPPGTRQVARVGALPVGQDLLVCVENLGSAPAVVMGEGPNMDVAYMRTRPRSTLDLLG
                     TAFDRASLFRPEWIGAWPFWVLAGALVLVAPLLLARALARAEREDLAGDDLDPVGPPR
                     A"
     gene            complement(8383..10749)
                     /locus_tag="VF752_15300"
     CDS             complement(8383..10749)
                     /locus_tag="VF752_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856960.1"
                     /translation="MDYLVAWLLLPLLVAVTSLGVGLGVSRLSGVRLPAGLTLPLGFA
                     ASLVLLSFPFALGLGQAWGGVVLAVATVAGLVLGWDRVPAALPGRWLTLGMLAVFGIY
                     IAPVVLSGDLTFGGYAFLGDTSVHFTLIDYVAKHGSTHVFEPPSTYAGVTQALIPTGY
                     PIDLHLELASLRWLTGTDLAAIYQPFLAATIALGVGPAMYLLGRIGMPRALAALSSVV
                     ALSAYLVFSYSLEGATKELAMVVLTLLGAALTLMFFDAERTLRLAVPLGITIAAAFGI
                     YSAGGLPWFGLMFLLAAGAAVVQGASLRSLVKPTLALGVATGVASIPAMLKAVDFYSQ
                     GTNLVNSSDASTRLGNLIGPLKSAQAAGIWLVGDFRLFTHHPGLTYSLIGVVAVLTLF
                     GVAQLVRRGSYALLLLLAAAFLAWLVVPGSPWIDAKLLLLLSPAVLLTALTGAWLLGR
                     GGHGIEATVLAAAVISGVFVSDALAYHDMYLAPNARLSELKKVGERFSGRGPTLYNEF
                     EEYGKHYLRGMRVNTAGDGFSPYPPELRDPAAGSYAQYHDLDEMKLPYVLRFPVIVMR
                     RSPVASRPPAPYTRVFQGRYFEVWQRPGGAAGLPSAHLPLGNATDSTARPACRDVRAL
                     ASQARSAGSSLVAAVRPPVVSLPVKQMQLPRGWPVGADGRVGPQTPGTASGSLTTSAG
                     AYRVWLRGTFGRGVDVRVDGRTVGHANGIQTPQQMAYLGLLNLGAGSHQVQLVRAGGG
                     LAPGNGRDEAYDTFFLEPVAPERLERVAPAAARSLCARRADWIEVVAG"
     gene            complement(10749..12941)
                     /locus_tag="VF752_15305"
     CDS             complement(10749..12941)
                     /locus_tag="VF752_15305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856961.1"
                     /translation="MNGWVTVAIPVLNGARYLEEVLTALRAQRVDREVEILIVDSGST
                     DGSVEIAERHGATVHRIDKKDFSHGGTRNWMMSLAKGDHVAFITQDATPASDRWLHAL
                     LEGFEVADDVAAVFGPHDARPDASHMIKSEMERHFATWGGGTEIDVQRLTRTPAELAE
                     YRKFPGRWTFFSDVNGAVARWAWEKVPYREVPYAEDQLLGRELIEAGFAKVFHPEARV
                     LHSHDYPPGQFLRRYFDEFRSLREVLQHKEPAGLKHSLWAVRGLAGADKRWLREQGLS
                     GRGLVKPLAVSLRHHTIRTAGAIAGSRADRVPRGLRRRLSLEGRDTFVPYDVPASPLL
                     DEPKPGMRKVKVTPDWTWEFVRKRFPPRPITLEPHAGRHTGPMTVAWVVPNWLVGSGG
                     HTTIFRLIRQLELRGHKCVIYVFDPFGREKRKNGRVLREEIREKFIPVEAEVFYGLDD
                     FDSADVCVATNWWTAYPVRDLPRCLEKVYLVQDDEPQFYPTSTESILVEETYRMGYRC
                     VAYTPWMADILTNKWGLEARWFECGTDLDTYEFAGPEGREPGVIAVYARRETERRAVD
                     LALMGLATLVERRPGIRPVLFGSNTKLSSPIPVLDLGVRPPAELAALYRRASAGVVFS
                     LTTHSLVAQEMMASGLPVVELEGENVSSALGGSGDLVMLAEKQPDSIADALEQVLDDP
                     EHAAAMARRAREFVETRTWERAGDQVESALREFLANPRVPAEAAAQPA"
     gene            complement(12938..13198)
                     /locus_tag="VF752_15310"
     CDS             complement(12938..13198)
                     /locus_tag="VF752_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856962.1"
                     /translation="MSTETGITTDWERRARDAEAALEESLRERNRLWEELQALKADAR
                     EVEAAKREVYRVETSASWKVTRPLRFAKTIWHKVKRRLDLDS"
     gene            complement(13218..14066)
                     /locus_tag="VF752_15315"
     CDS             complement(13218..14066)
                     /locus_tag="VF752_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8856963.1"
                     /translation="MTTQAAHFGRPIKGPSALSGGRGRLLSLTWTLAFTDFKLKFFGS
                     VLGYLWQLVRPLLLFGVLYVVFTHVVRLGSDVQFYPVVLLTGIVLYTFVAEATGGCVS
                     AVVDREGLVRKIQFPRIVIPLSVIVTAYLNLALNLIAVFIFAIASGVDIRWSWLELPF
                     LLAFLGVFATGIGMALSALYVRYRDVRPIWEVGLQLLFYASPVIYPVEKLPNATDQHL
                     AMCNPLAAVIQQVRHSVIDPTAPSAAQAIGGAPRLLIPIAITFGLFVLGFWIFNREAP
                     RIAEEL"
     gene            complement(14063..15307)
                     /locus_tag="VF752_15320"
     CDS             complement(14063..15307)
                     /locus_tag="VF752_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8856964.1"
                     /translation="MSPSQDNAPAVFVDSVSKRFRIPHERVHTLKERALHPFRRATHD
                     DLQALRDVSFAVQPGEFFGIVGRNGSGKSTLLKCLAGIYAVDSGVIYVNGRVATFIEL
                     GVGFNPDLAARDNIVLNGVMLGLSPAEARERVDRVLDFAELHEFEELKLKNYSSGMQV
                     RLAFSVMIQVDADILLIDEVLAVGDAAFQQKCFDEFRRLREERKTILFVTHDMPAVTR
                     FCHRAMLVERGELVTIGDPGEVADRYISVNFPGNALAAAPGGQPVSEYDADILDAWFE
                     EEHGDRHEYLPQGRPATFRARVRFNTEVRDPVFVLGLSNEHGHKVFATSTTWVDENTG
                     QFRAGDEAVLGVTFENQLVPGRYTANFQVARPGSGAMVLAQLDPAASMVSTGTRNSGG
                     IVDLPHEIDIRRPVRAPEESPA"
     gene            15362..16309
                     /locus_tag="VF752_15325"
     CDS             15362..16309
                     /locus_tag="VF752_15325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856965.1"
                     /translation="MTPEATVVVPTHHHGELLLRSVGCALAQTVGDLEVLIVGDGATE
                     ETREAALQLERSDARVRFFDNPKGPRLGEVHRAAALEQAQGRLVFYLSDDDLWMPDHV
                     ERMTAALDAAHADFVHAPPVWVMPDGELLVWLVDLAQPWFRELALRESRIPLSSGAHT
                     LAAYRRLPVGWNTTPEGQYVDFMMWRRFLEQRDMTFAVSRTHTVMHFPGSVREGISNA
                     ERADELDDWLPATRDLAARAAYLEQLAAHLHERANWLDSHAGELESWAQNRSQVVDLR
                     NEQLSQAERERQRLEEVIRAMKSSRRWRAGERLRRLSRP"
     gene            complement(16296..16844)
                     /locus_tag="VF752_15330"
     CDS             complement(16296..16844)
                     /locus_tag="VF752_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856966.1"
                     /translation="MGELISSEAVSPAGQPDPPTVDSQGRRLSTGIDGVELERLVVHD
                     DHRGSLVEVMNFERPFWREPVIYAYSVTIRPRRIKGWGMHKLQTDRYYVASGNIRVVL
                     LDGRLESPSHGALSEFHFTDRTPGLLAIPPGVWHADQNWGDSDAMIINFPTRPYDHVN
                     PDKNRVDPHSGEIPFDWALRDG"
     gene            16896..17603
                     /locus_tag="VF752_15335"
     CDS             16896..17603
                     /locus_tag="VF752_15335"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8856967.1"
                     /translation="MQAPGTLGLSEELRRFVEEAPYERRTIVEFVGRAAGSLLSGARV
                     LDVGAGDAPYRELFEHVDYVTTDWSESPHEQAREADVIASAERLPVEDQSFDALLFTQ
                     VLEHVADPRAVLGEAHRVLRAGGRLFVTVPLVWELHELPYDYWRYTPASLETLAAGAG
                     FTDVDVQARNDCFTTLAQLMLNARHVMGRAPDGLDSRRDEAAALLEELAARVAELAPL
                     DVEGILPLGFSMTARRV"
     gene            17600..21247
                     /locus_tag="VF752_15340"
     CDS             17600..21247
                     /locus_tag="VF752_15340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856968.1"
                     /translation="MTPGGRRPVLYLAPWVDYGGTDKGTIDWFRWLDRDRYAPSLIAT
                     QPSLNRRLSEVIPYAEEVWALSELMRGSDFPGAIFDFIHTRGIDVVHIMNSRMGYDLL
                     PDLAALPKPPAVVVQLHVEEQDKSGYVRYVTTRYGNLVDAFSVTSEHLANAVSAYDVP
                     RAKTRVIYTGVDAEQEFNPERVRPIDGIEQGPVHILYPGRLVPQKDPLLMVEVIAALA
                     VTGSDFRVHVVGEGQLEPAVHEAVRERGLERFVRFYPPTNEIARWFAACDLLLMTSVF
                     EGVPYVVFEAMAMGVPIVAPALPGNAELMDGEAGALIDPRDDVGAYVRALAELVGDDE
                     RRRELGWRARRRVQERFSLQRMAADHADLYDELLAGRAKPAKPDAPVRHELPEPLRLR
                     GRPSRGQPPVSVIVPCFNHGRYLPECLASIREQSYDGEIQTIVVDDGSTDPETVELLT
                     ELERGDDVEVLRMSENSGPSAARNAALERACGRFVLPVDADNLLLPEALEKVVGQLQN
                     AGETVGYVYPSPQYFGNRLDWFRAPEFNLHRLLDGNFADTSSLLDRQIFDAGFRYPEV
                     GLGHEDWDFALSLAEHEVSGEPARERTLLYRKRGFTRSDLVEYAADEFKERLPERHGA
                     LYERAADAKSRWSPALSIVTLRPVDPEGESRGRLGARVFSQSCSDFELIVRSDTLWPD
                     PGSGPHLRRIPAQLARSASEALQTGWHAARGRYMLVTAGTGSELLADPAFVEKVLRVF
                     SSNAELQAIGLTDAGEKGRFPLRLIEPGEGIEPEPHAIVWRADCDELMPPATALQEGR
                     ELASLATAVSGLLVTQWRHAPAPPRFRPDATGGVAPSVQRPSKGRPSEAEDRRYRLEA
                     APALPRVPGGVRRWSLSPTWMPPGTLPLVRHASRDGLRRIVTRSELSPPGFSPEFHLG
                     MAHAAAVPGTVRLEWSPAPDYAAVAIERPEGDPEFTLGYLELAAFPLLQPLILARHGA
                     SGQHVVVAGHGDPLLAHVESLGTLGFAESWPVSPRRIPHARLDYGLLGLVRTVDLDAR
                     THRYGVGAVPPGTVAGELGSLHIQPGDQSIPVWLSADGVFSTDRYTLPSHAPGRKSAA
                     KWAVAPLAWRGSGPLGPRVRAAVRRGLTAVSALSGGARAEAERQGAPVGYLHPPDTAV
                     ESVPLYSAWLPVTGDQLLTSHPLEAADMGYVETTLLGSLLAAAPMTGRLGVEDVGVPW
                     ASRFGHNARRS"
     gene            21244..22122
                     /locus_tag="VF752_15345"
     CDS             21244..22122
                     /locus_tag="VF752_15345"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HEX8856969.1"
                     /translation="MSEEADIQERVDDALAGIRNAVRRQLGVDRPPSVEVEIAALPRF
                     SPDLPEELHPEERARLEREAEELQPWLQGPFLLGGDLLVGGAWRNDQRWLGLADHVPN
                     LQAKRVLDVGSNAGYDPFMWNLRGAEYVLACEPFEFHRQALFLESVYRTGIDFQQIGW
                     QDLDAEKQGRFDLIHCHGVLYHEPHPMLMLKRLREMLAPGGTMLFGSMMLASPELSEY
                     ARFVPGAYYGDETWWWVPGRLCMRWMLEAAGFVSEEEFGIAKGPLGDFPTINGYFSLA
                     GGEPSPLIDDPLALKP"
     gene            22119..24377
                     /locus_tag="VF752_15350"
     CDS             22119..24377
                     /locus_tag="VF752_15350"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX8856970.1"
                     /translation="MTSSRQVVTGRSARSGGALAQAAGWLASWRWTGLAALTLAFVAY
                     YWQVIFAGKLVSAADLVNLYYPYAATAGPGFQPHNPALTDSVVSLAPAMTYVRDSVWS
                     GQLPLWNPYTEFGMPFFAQSQWGILSPLQLPFLAPPKLFGVEVAQTLTGMLKVAAAGS
                     GAYLLARALRCSKPAAVFAGIAWMLGGYTTAWLSMPATSATVMLPWICLGVEATLRWR
                     RPWLAAAGLASVMAVAVFGGHPEALLNAFYGVVIYGVVRLLQNRRHMRGQVRLRLAAI
                     GTAIVLALGLVAVIIVPAAELLHHSVDSTLRELQYRGTAIGWNNVIQVFDPHHFNDPT
                     HTGTFYSAYSAPWFAGVVTALAALVALVFRDRRIVPFVLMCVLALGIATNSWPWQRVE
                     DAIPTLGGVAKSAPLVLWAFGLSMTGALGVDRVLRFIRGRSTVPWLAPAAGAALCLVL
                     FVELYSWGHDYNPRVPRSMVGVPRPPGIDAIQAGRGAPRTVFLGHTMPFLLPMQFERP
                     DARGYGQPTRRDYDLFMRLAVTDLRSVFNPSELGYPIANIKPHQLAVLRAANTRYAAW
                     TDGRWKLQGYDQVSQGPLNVAQTNNAMGRAYLVSDYVTEPKLESAIQTVAGESFDPNR
                     QVVLDEDPAGARPDPLRRTPGPPPQVTHYENNHVVVRARLARPGLLVLSDSDYPGWRA
                     SVDGKSVRMYRANGLFRAVSLPAGEHVVHFDFRPASVYVGAAISLVSVLFIIGLALFG
                     RRRELEPAEDRG"
     gene            complement(24324..25616)
                     /locus_tag="VF752_15355"
     CDS             complement(24324..25616)
                     /locus_tag="VF752_15355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8856971.1"
                     /translation="MATTVEQPAAPPPSEARRTRSRLPLDPWILGLSAIGFALRTVYA
                     VLAHGPENIGDQIQYAKIAHDFSSWWSSADAIRTPGYPLLLAINFSLGQGTNVIRVEQ
                     ALIVSVAALLVALLAARFCGLTAGRATAALLALLPPLVMLPALLLPDALAASLFAAAC
                     FCLLWGRGARRQLWWFAGAGAFAAAAALVRPNLLLGLGVIVVAAPLLVHRPRVRALAA
                     AAVVLPVVALYAPWIARNFSELHKPAPVGINPGAEWVAAAGVHLPIDRTDGPKGAYLR
                     SLHFYTPGNMADGPLNITAAQSGRVKAFDTLRDNVEHRPLKQLSASAFWQRELWLEAF
                     DDHAQYGRPTKIPYWLSLGVQAFMLVLALGGLWLLRGTALARLVLAASLVLAAPFLIH
                     LPEPRYGAPMLLLLAAPAGAALAAIFGRLELAPPTEQR"
     gene            complement(25619..>27072)
                     /locus_tag="VF752_15360"
     CDS             complement(25619..>27072)
                     /locus_tag="VF752_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856972.1"
                     /translation="AATLYPATGGSGFFDAIKFFWDFTSYRPVLVLYVPLTHEVFGAH
                     FTAHLAWSIGLAVAMSASLYLLLRTLRMEPLHAGLISVLVLLFPQSDSTRLWSTASMT
                     SLAITIFLLGVVVALHAFEARGRRALLLHAASMALYLLSVMTYEVTAPAALSVIVLYA
                     LRFPLRVVWKRWIADVGLIGLTLLLVTRSGNHEIQTFAQMRHHVKLIADQGVTVFART
                     VVPFGDPARWLVAGVVAAILVAALVVWARTRDRMLRRWLVVAGVAVWGVVVGYAMFIP
                     ADAYYSPLTAGLGNRTNVLASIGLVTLVYSAAIIAGTLLARGMRAERRSPTLLAAGAA
                     ALVAIVIGVGYAQDLEHDIDRWDTAYQMENSVITVLRGTLKHPPHGSTIYTFQAPGFY
                     TPGVPVFSASWDLNGAVKMHFHDRTLAAFPVLAPLACGATTVTSTVGMPPASYGNAVF
                     VNIASGQAEQIMSRKQCESALKRFRPGVFEAAR"
BASE COUNT         4188 a         9310 c         9530 g         4044 t
ORIGIN      
        1 gttctacccg gccaatatct ggccgcacaa gaaccacgag cgcttggtgg atgcgctcac
       61 ccgggtggag gaccgcgagc tggcgctcgt gctgagtggc caggaccacg gcaagctgcc
      121 cgcgctgctc gagcgagcga gggcagcagg cgttggcgac cgcgtgatgc acgccgggta
      181 cgtcgagcgc gcgcagcttc cggcgatcta ccgccgtgca accggcgtga ttttcccgag
      241 cctgttcgag gggctcggca tcccgccgct cgaggcgcag gcctgcggct gcccggtcgc
      301 ggcggcgcga gttgcctcgc tccccgaggt gctcggcgag cgcgcgctct ggttcgaccc
      361 gcgcgacacc gacgccatcg cgggcgcgat cgacgcgctc gccgcgggca gggcgccggg
      421 cggtactccg ccggagagct tctggagccg cttcacctgg gaggccgccg cggcggatca
      481 ccggcgggcc tacgcgctgg cggcggggca gcgctccggc actaggatgg ctgtccgatg
      541 agcgacctgt cgggcaggag ggtgctggtg acgggcggcg cgggctttct cggctcgcac
      601 gtcgtgaacc tcctgcgcga gcgcggcgcg gagctgatcg tgccgcgcag cgcggagttc
      661 gacctggtgg tcgaggacga cgttgagcgg ctcatcgccg aggcgcgccc ggagatggtc
      721 atgcacctcg cggcgcgcgt ggggggcatc ggcgcgaacc agcgcaatcc gggcgcctac
      781 ttctacgcga acctgatgat gggtgcgctc ctgatggagc actcacgccg ggcgggagtc
      841 gagaagttcc ttcagctcgg gacgatctgc gcgtacccga agttcacgcc ggttcccttc
      901 cacgaggacg acctgtggaa cggatacccc gaggagacca acgcgccgta cgggatcgcg
      961 aagaaggcgc tgctcgttca ggcgcagtcg taccgcgagc agtacggatt caacgcggtc
     1021 tacctgctgc ccgtgaacct ctacggcccg aaggacaact tcgaccgcga gagctcgcac
     1081 gtgatccccg cgctcatccg caagatgatc gaggcggagg agaacggcga cggcagcgtc
     1141 gtgctttggg gggacggctc gcccagtcgt gagttcctct acgtggacga ctgcgccgag
     1201 gcgctcgtgc tcgcgctcga gcgctacgac aagcccgacc cggtgaacat cggcgccggc
     1261 ttcgagatca cgatcaggga cctcgccgag ctggtgcgcg agatcaccgg cttcgacggc
     1321 gagatcgaat gggacacctc caagcccggc gggcagccgc ggcggatgct cgacacctcg
     1381 cgcgccgagc gcgagttcgg cttcaaggcg atgaccggct tccgagaggg tctccagaag
     1441 acggtcgact ggttccgcaa gaaccgcgac tgggtcgcgg aggatgagcg gcggcgcaag
     1501 gagtccgagc gacgggccat ggcggcggcc ggcctgctca cgggctcacc ctgacgggcc
     1561 cgcgggttat ccttcgcgcg gcgcaaccct cgcctggcag gccggttgaa ctggcgggaa
     1621 gacgcccagc gccgcttgct cagcccacca tgcggaagag ctatctagcc atcatcccgg
     1681 ctctcaacga ggagcacacg atcgcgtccg tgatcgacgg cgtccggctg cacgcgccgc
     1741 agttcgacgt cgtggtgata gacgacggct cgagcgaccg cacaagggag gtctccgagg
     1801 cggcgggcgc gccggtgatc cggcatccgt tcaacctcgg catcggcggc gcggttcagt
     1861 ccggattcag ctacgcgcag gagaacggct acgacttcat ggtgcaggtc gacggcgacg
     1921 ggcagcacga tccagccgag gtcgccaagc tgatcgaccg gatggcgcaa aacggcgtgg
     1981 acatggtctg cggctcgcgc ttcatggaga agaccggcta cgcggcgccg cgcgcgcggc
     2041 gcaccggcat tcggatcttc tccgcgatcc tgtcgaggat cgtcggccag caggtcactg
     2101 acccaacctc gggcttccgg ctcttcaacc gtcgcgcgat cgggttgttc gcgcgcgact
     2161 acccgcacga ctatcccgag gtcgaggcgg tcctgatgct gcacttccat cagctgcgga
     2221 tgtgcgaggt tccggtgacg atgtaccagc gcggcggcgg tgtgtcgtcg atcagcactg
     2281 ggaagtcggc ctactacatg atcaaggtgc tgatggcgat cgtggtcgga ctcgtgcgcg
     2341 ctcgtccggt cgtcgaaccc ggtgacgagc ccccggtcgc cgcggcgcac ggaatctgat
     2401 gggcacgcgc atccagatcg tctcgatcgt ggcgacggtc gcgctgttcg tgatcgtgtt
     2461 cgagctggtg cggcggcgcc ggcttcagga gcgctatgcg ctgctctggc tgttcgccgc
     2521 ggtcgtgctg ttcgggctcg ccgcttggca gggcctcctg tcgaagatcg cgagcgcggt
     2581 cggcatcttc tacccgccgt cggcactgtt cgtgatcgcc ttcggcttca tcctcgtgct
     2641 gctgctgcac ttctcggtgg ccgtgtcgcg attgtccgac cagaccaagg tgctggcgca
     2701 gcgcctggcg ctcacggaga agcgactgag ggacctggag gccggcgcgg agccagaggt
     2761 gcgcgaggag gaacgcgcgc ccgagacggt cggccgcgcg cctacgccgt agcggcgcgg
     2821 cggcggacga ccgacgtcag cagacgccat tcgtccggcc acgccacgcg ggtcgccact
     2881 acggcgagtg ccaccgccag cgcggccgcc ggcaccacca ctgctgcgtc gtagccgaga
     2941 gccagcacga gcgcaaccga cggcgcaaaa gcaaccgcca tcgcaccaac cgcacggccg
     3001 gtgtcggccg ccatgtggcg caccacgcgt cgcgcgtccg cggcgaacgc caagtgaagt
     3061 tgagcggcgg tggcggccag caccacgagc gactgcgcga gcgcgatgcc cctgatgccc
     3121 cacgcctcgc gcccggcgat cgccagcggg acgagcagca gcacctgcgc caccccaatc
     3181 agcgcgagct cccggagccg cgcgcgggcg agcagctcca cgaccgcgaa cgtgccggcc
     3241 gcggtgccga gcacccatcc cgacagaagc agcagtgtct ccacgaggcg cacggtgtcg
     3301 tcgtgaccga aggcgccgcc cagcacgaac tcgaggaccg gctggcccac gagcgcggca
     3361 accgccagca ccggcgtcac cacgacgacg gtcacgcggt aggtcgccat cgcgctctgg
     3421 gccatcacct gctcggagcg cgcctcggaa gagagcagcc gcgggctgcg caccatcgcc
     3481 gccgaggtgg cggtcatcgc caccaggatc gccgcgctga agtacgcgta cgcgtagagc
     3541 gtcgcctccc ctgagcccgc gcgtgaggcg acggccacgc agatcacgta gccgaggttc
     3601 agcgccacga aggtcgcgga cgcgagcgag aggtcgcgca cctcgcgcgc aatggatcca
     3661 gcgccgccgg ggctcgcggc tggccggccg cccgattcct gcagaaacgc ccagccgagg
     3721 atcaccgcga gcatcacgcc tgccacgtcg aggcctatcg caacggcttc gatgcccacc
     3781 gagctttcaa gcgcaaggaa cacggcgacg ctgacgagcc cggtcacgag gtagctgacg
     3841 ccgatcagca cgaagcgccc gcgggtcgcg aggatggccg cgagcatcga gccgaacagc
     3901 tgcccggcca cagcgatcca gagcagccga agtgagtgag cggccacgtg ccccggatcc
     3961 gacctcacga gcacggaggc gaccgggccg gcgagcagca ccatcggcac cgccgccacg
     4021 agcacgatcg cgccgacggc ggcgagcagg cgcgcggtcg ctgcagggtc gccggcgtcg
     4081 gtgagcttgg ggagcgccgt gatcctgaac gtctgcccga agatgatcag cacggtggcg
     4141 actccgtagg cagcgaagaa ggcgtcggtg cgtcccgact tgccgaagat gtgggcgatc
     4201 aggagcgcga ggacgccgcc cataaccatc gccgacgcct gccccagcga gatgatcgcg
     4261 gtgtgacggg cgtcgtcgga ggcgggcggg ccggtggtgg gcgcggcagc gcgcggcacc
     4321 tcgtcggccg gcacgacgcc ggtctcgcgc ggtgggacgg tcacgcgccg cgctcgcgga
     4381 ggtagcgcat cccgcggtac acggcgggaa tcagccggct gatcaccacg ttcgctccga
     4441 cggccacggg atgcccggct ccgtgctcac gcgcgttttc cgcatgctcc acgaactgcg
     4501 tctcgaagcc cgacatgctc agcgagccgg gcgccatccg gaacgccgcg atctcctcgt
     4561 cgatgaacag cggccgccag ccctggcggc cgagccgcag ccagaagtcg taatccatcg
     4621 agtaccggaa gcgctcgtcg aacatgccca ctcggtcgaa cgcctcgcgc cggacgaaag
     4681 tcccgggcgc cgagacgaag ttctgcacga ggtgcagcgg gaacgaccag cgccggagga
     4741 agaagtcctt gtaggcggtc actgccgtgc ggatctcgtt gccgttgccg tcgatgatcg
     4801 gacagcggcc gaagccccac atcgcctcag ggtgccgggc gaactcctcg ccgacacgtc
     4861 gaagtgcacc cggcagatac acgtcgtcgg cgttcagcca gccgacgagc tcgcctgtgg
     4921 ccatgcgcac gcccttgttc atcgcgtcac tcaggccgcg gtcgggctcg gagatgaagc
     4981 ggacgccctc ggcgcgctcg aggatctcga ccgtgccgtc gttggagccg ccgtcgacaa
     5041 cgatgtgctc gagcttggga tagccctgtg agcgcacact ttcgagcgcg gtctcgaggg
     5101 tccccgccgc gttcaggcac ggcgtgacga tggacagcga cgggacgtcc atgagcgctg
     5161 ttgtacttta ggcggcccta tggcagggaa gaaggcgctc ataaccggca tcacggggca
     5221 ggacggctcc tacctcgcgg agcttctgct cgacaagggc tatgaggtcc acggcattgt
     5281 gcgccgctcg tcgagcttca acacggaccg gatcgaccac ctctaccggg atccgcacga
     5341 gcagggcgtg cgcctcttca accactacgg cgaccttgcc gattcggtgg cgctcaccaa
     5401 gctgctctac gagcttcagc ccgacgagat ctaccacctc ggcgcccaga gccacgtgcg
     5461 cgtgagcttc gacatccccg agtacacagc cgacatcgtc gggcttggaa cgatccgcat
     5521 gctcgaggcg atccgcgagg ccgaggtgaa gacgcgcttc tatcaggcgt cctcctccga
     5581 gatgtacggg aaggtgcagg aggtcccgca gaccgagacc acgccgttct acccgcgatc
     5641 gccgtacgcc gtcgcgaagg tcgcggcgtt ctgggcgacg gtcaactacc gcgagtccta
     5701 cgggatgcac gcgagcaacg ggatcctcca caaccacgag tcgccgcgcc gcggagagac
     5761 gttcgtcacc cgcaagatca cccgggccgt cgctcggatc aagcacggac tccaggacaa
     5821 gctctacatg ggcaatctcg acgccaagcg cgactggggg tatgcgaagg actatgtcga
     5881 ggcgatgtgg ctcatgctcc agcaggacga gccggacgac tacgtaatcg ccacggggca
     5941 gacccacagc gtgcgcgagt ttctcgagga ggcgtttggc tacgccgacc tcgactaccg
     6001 ggactacgtc gagatcgacg cccgctactt ccgtcccgct gaggtggacc tcctgctcgg
     6061 agacccgacg aaggccaagg acaagctcgg ctgggagccg cgcgtgacct tcaaggagct
     6121 cgtgaagctc atggtcgaat ccgacatgga agaggtgcgc gcccagctcg agggcggcgc
     6181 ggcggccgtc cgccgctaac cggcctgtca cactgccgcc ggacgcgttg gctgtagaag
     6241 ctccagcacg ggaggaggct cagccgcgcc ctcgaggcgg gctcctggcg cgggcccgcg
     6301 cacgcgtgcc ggcgccgctg gcgttgctgc tcgcggtcgt ggcgctgttc tgcctcgcct
     6361 gggtcctgat cgtcccggct ggccaggtcc ccgacgagga gatgcacctc cagtacgcgc
     6421 tcacgctcgc ggagcgcggc gagctgcccg gacacggacc caacgggttc tcgacgcagc
     6481 agtggaccgc cgaggtcgcc acaaactggg agcgcatgcc cggggcgccg gagcgaaagc
     6541 cgccctggac gaacggcgag tacgagaaat ggaaggccgc cgacgccgca ctgccgcccg
     6601 gatcgaagag caacggcggc ggctacatct ggcagggcga caacccgccg ctctactacg
     6661 gctacgaggc ggccgcgtat cacgcctcgc ccgggcgtgg cttcttcgac cgcctgtact
     6721 tcatgagggt gatgtcggcg ctctgggtcc tcgtgaccgc caccggcgcg tggctgctcg
     6781 ccggcgagat cttcgggcgt gaccggccgc ttcagcttgt cgccgccggg ttcgcgggcc
     6841 tgcttccgat ggtcgacttc gtcagctcgg gggtgaaccc cgacgcgatg ctctacgcgt
     6901 tctggagcgt cgcgttctgg ctgggagcca ggatcctgcg gcgtggcctc accgtgcgcg
     6961 acggcgtggc cctctgtgct ctggtgggcg tggcgttcgc ggagaagtcg acgagcattg
     7021 cgctcatccc gccgatgctg ctcgcgctgg cggtcggctg gcgacgactc tcgcggactc
     7081 gcccgcaagc gcgccgggtc gcgactgtcg cggtgctggt gtcgcttgcg gccttcgcgg
     7141 tcccagctgg cgcctgggtg gtgaccgcca aggcactcga ccgtccgatc acgaatcgcg
     7201 cgccgcatta cgtcggcaag ccggctcctc aactcacctc tatccaaacg gaccgccagt
     7261 tcctgagcta cgtctggcag ttctatctgc cgcgcctgcc gttcatgcaa cccatcccgc
     7321 tcctaggctc gggcggcgac aagctgtacc gcgtctgggt ctcccaggtg tggggcaact
     7381 tcggctggca ggaggtgaac ttcccgccct gggcgtacaa gggcttcgcc gcgctgtccg
     7441 tgctgctgct cgccgggggt gcggcagcgg cactgcgcgc gcgaaggcgc ggccacttcg
     7501 acctcgcgat cgccgcctac ttcgcgctcg cgatcgtgag cctgctcttc ctcctgcact
     7561 gggcggagta cacgatcatg agcctcgagg ggatcgcctt caatcagggc cgctacctgc
     7621 tgccgctcgt ggcgctggga ggagtggtcg tcgcggcctg cgtgtcgctc ctgcgacctt
     7681 cgctacgcac gcccgcgacc ggtgtcgtgc tgggttcgct ggtggtgctc cagctcttct
     7741 cgctcggcct cgtcgtggag cgttactttg tctaggcggc tgctcgttcc cggcgtcgtg
     7801 gtcgcggtgg tcgccgccgg cgtgctcgcg ctcgtgctcg tcgcagccgg taacgagcag
     7861 cacatcgcct atccgctcgg ctttggcgac gagggcgttt cggcgcagat cgcgccggga
     7921 gcgcacgcgt gccagcaggg agcagcccac gttccaacct cggccgacgg cgtgcgactc
     7981 acactcggcc aacccggatc gacgccgccg gtgcgcgtga cggtgttcgg cgccgaccga
     8041 cgcgtgctgg gtcggggcga catccgcggc ggctacccgc cgggcacccg gcaggtcgct
     8101 cgcgtcggcg cactgcccgt cggccaggac ctactcgtct gcgtcgagaa cctggggagc
     8161 gcaccggcgg tggtgatggg cgaagggccg aacatggacg tcgcctacat gcgcacacgc
     8221 ccgcgaagca cgctcgacct gctcggcacg gcgttcgatc gcgcctcgct gttccgcccg
     8281 gagtggatcg gggcctggcc cttctgggtg ctggcaggcg cgctcgtcct ggtcgcgccg
     8341 ctgctcctgg cgcgcgcgct cgctcgcgcc gagcgcgaag atctagccgg cgacgacctc
     8401 gatccagtcg gcccgccgcg cgcatagcga gcgggctgcg gcgggcgcga cgcgctccaa
     8461 gcgctccggc gccacgggct cgaggaagaa ggtgtcgtag gcctcgtcac ggccgttgcc
     8521 gggtgcgagc ccgccgccgg cgcggacgag ctgaacctgg tggctgcctg ctccgaggtt
     8581 cagcaggccc aggtaggcca tctgctgtgg cgtttgaatt ccgttcgcgt ggcccacagt
     8641 gcggccgtcc acccgcacgt ccacgccgcg gccgaacgtg ccacgaagcc aaacgcggta
     8701 ggcgccggcg ctcgtggtca gcgaaccgct cgcggtcccc ggcgtctgcg gacccactcg
     8761 cccgtcggcg ccgaccggcc aaccgcgcgg cagctgcatc tgcttcaccg gcagggacac
     8821 gaccggcggc cgcacggcgg cgacgagcga cgagcccgcc gagcgcgcct gcgacgcgag
     8881 cgcgcgcacg tcgcggcacg cgggccgggc cgtggagtcg gtcgcattgc cgagcggcaa
     8941 gtgcgcgctc ggcaggccgg cggcgccccc ggggcgctgc cacacctcga agtagcggcc
     9001 ctggaacacg cgcgtgtacg gcgcgggcgg cctgctcgcc accggcgagc ggcgcatcac
     9061 gatcaccggg aagcggagca cgtacgggag cttcatctcg tcgaggtcgt ggtactgcgc
     9121 gtacgagccg gccgccgggt cgcgcagctc gggcggatag gggctgaagc cgtcgccggc
     9181 ggtgttcacc cgcatgccgc gcaggtaatg cttgccgtac tcctcgaact cgttgtagag
     9241 cgtcggcccg cggccgctga agcgctcgcc caccttcttc agctcgctca gccgcgcgtt
     9301 cggcgcgagg tacatgtcgt ggtaggcgag cgcgtcggac acgaacacgc cgctgatcac
     9361 cgccgcggcc agcacggtcg cctcgatgcc gtgaccgcca cgacccagca gccaggcgcc
     9421 ggtgagagcg gtgagcagga cggccggcga gaggagcaga agcagcttgg cgtcgatcca
     9481 ggggctgccg gggacgacga gccaggcgag gaacgcggcg gcgaggagca gcaagagcgc
     9541 gtacgacccg cgccggacga gctgcgccac accgaacagc gtgagcaccg cgacgacgcc
     9601 gatcagcgag tacgtgagcc ccgggtggtg cgtgaagagc cggaagtcgc ccacgagcca
     9661 gatgcccgcg gcctgcgcgg acttcagcgg gccaatcagg ttgccgagcc gcgtggaggc
     9721 gtcgctgctg ttgacgaggt tcgtgccctg cgaatagaag tcgacggctt tgagcatcgc
     9781 cgggatggac gccacgcccg tggccacgcc gagcgccagc gtcggcttca ccagcgagcg
     9841 cagcgaggcg ccctgcacca ccgcggcgcc ggccgcaagc aggaacatca ggccgaacca
     9901 cggcagcccg cccgcgctgt agatgccgaa cgctgccgcg atcgtgatgc cgagcggcac
     9961 cgccaggcgc agcgtgcgct ctgcgtcgaa gaacatgagc gttaaggcgg cgccgagcag
    10021 ggtcagcacc accatcgcga gctccttggt cgcgccctcg agcgagtacg agaacacgag
    10081 gtatgcgctc agcgcgacca ccgacgagag cgccgcgagc gcgcgcggca tgccgatccg
    10141 cccgagcagg tacatcgcgg ggccgacgcc gagggcgatc gtggcagcca ggaaaggctg
    10201 gtagatcgcg gcgaggtccg tgccggtcag ccagcgcagc gaggcgagct cgaggtggag
    10261 gtcgatcgga tagccggtcg ggatcagggc ctgcgtgacg cccgcgtagg tggacggcgg
    10321 ctcgaacacg tgcgtgctgc cgtgcttcgc cacgtagtcg atcagcgtga agtgcaccga
    10381 cgtgtcgccg aggaaggcgt agccgccgaa ggtgaggtcg ccggacagca cgacgggcgc
    10441 gatgtagatg ccgaacacgg cgagcatgcc gagcgtgagc cagcgccccg gaagcgccgc
    10501 gggcacgcgg tcccagccga gcacgagacc ggcgacggtc gcgacggcga gcacgacgcc
    10561 gccccaggcc tggcccagcc ccagcgcgaa cgggaacgac agcagcacga gcgaagccgc
    10621 gaatccgagt gggagagtga ggccagcggg cagccgcacg ccgctcagcc gcgagacgcc
    10681 gaggcccacg ccgagcgagg tgacagccac cagcagcggc agcagcagcc aggcgaccag
    10741 gtagtccatc aagcaggctg cgccgcggcc tcggcgggca cgcgcgggtt cgcgaggaac
    10801 tcgcgcagcg ccgactcgac ctggtcgccc gcgcgctccc acgtgcgcgt ctccacgaac
    10861 tcgcgcgcgc ggcgcgccat cgccgctgcg tgctcagggt cgtcgagcac ctgctcgagc
    10921 gcgtcggcga tcgagtcggg ctgcttctcg gcgagcatca cgaggtcgcc cgagccgccg
    10981 agtgcgctcg acacgttctc gccctcgagc tccacgacgg gtaggccgct ggccatcatc
    11041 tcctgcgcca ccagcgagtg ggtggtgagc gagaagacca cgccggcgct cgcgcggcgg
    11101 tagagcgcgg ccagctcggc cggcggacgg acgccgaggt cgagaaccgg gatcggcgag
    11161 ctcagcttcg tgttcgagcc gaacagcacc ggccggatcc ccggacggcg ctcaaccagg
    11221 gtggcgagcc ccatgagcgc gaggtcgacc gcacgccgct cggtttcgcg ccgcgcgtag
    11281 acggcgatca ctcccggctc gcgtccctcg ggcccggcga actcgtaggt gtcgaggtcg
    11341 gtgccgcact cgaaccagcg cgcctcgagc ccccacttgt tcgtgaggat gtcggccatc
    11401 cagggcgtgt aggccacgca gcggtagccc atgcggtagg tctcctcgac caggatcgac
    11461 tcggtcgagg tcgggtagaa ctgcggctcg tcgtcctgca cgaggtagac cttctcgagg
    11521 cagcgcggca ggtcgcgcac cggatacgcc gtccaccagt tggtggccac gcagacgtcg
    11581 gcggagtcga agtcgtcgag cccgtagaac acctccgcct cgaccgggat gaacttctcg
    11641 cggatctcct cgcgcagcac gcgcccgttc ttgcgcttct cgcgcccgaa cgggtcgaac
    11701 acgtagatca cgcacttgtg cccgcgcagc tcgagctggc ggatcagccg gaagatcgtg
    11761 gtgtggccgc ccgagccgac gagccagttc ggaaccaccc acgccactgt catcgggccg
    11821 gtgtggcggc cggcgtgcgg ctcgagggtg atcggccgcg gcgggaagcg cttgcgcacg
    11881 aactcccagg tccagtccgg cgtgaccttg accttcctca tgcctggctt cggctcgtcg
    11941 aggagcggac tcgcgggcac gtcatacggc acgaacgtat cgcgcccttc gagcgacagg
    12001 cggcgccgta gcccgcgcgg cacgcggtcg gcgcgcgagc cggcgatcgc gcccgcggtg
    12061 cggatcgtgt ggtggcgcag cgacacggcc agcggcttca cgaggccgcg gccgctcagg
    12121 ccctgctcgc gcagccagcg cttgtccgcg ccggcgagcc cgcgcaccgc ccacagcgag
    12181 tgtttgagac cggccggctc cttgtgctgc agcacctcgc ggagcgagcg gaactcgtcg
    12241 aagtagcggc gcaggaactg gcccggcggg tagtcgtgcg agtgcagcac gcgcgcctcg
    12301 ggatggaaca ccttcgcgaa gcctgcctcg atcagctcgc gcccgagcag ctggtcctcg
    12361 gcgtagggga cctcgcggta gggcaccttc tcccaggccc agcgcgccac cgcgccgttc
    12421 acgtcggaga agaaggtcca gcggcccggg aacttccggt actccgccag ctctgccggc
    12481 gtgcgcgtca gtcgctgcac gtcgatctcg gtgccgccgc cccatgttgc gaagtggcgc
    12541 tccatctccg acttgatcat gtggctcgcg tccgggcgcg cgtcgtgggg gccgaacacc
    12601 gccgccacgt cgtcggccac ttcgaagccc tcgagcaggg cgtgcagcca gcggtcgctc
    12661 gcgggcgtcg cgtcctgggt gatgaaggcg acgtgatcgc ccttcgccag cgacatcatc
    12721 cagttgcgcg tgcccccgtg cgagaagtcc ttcttgtcga tgcggtgcac ggtcgcgccg
    12781 tggcgctcgg cgatctcgac cgatccgtcc gtggagccgg agtcgacgat caggatctcc
    12841 acctcgcggt ccacgcgctg agcgcggagc gcggtcagca cctcttcgag gtagcgagcc
    12901 ccgttcagca cgggaatcgc gaccgtgacc cagccgttca cgagtcgaga tcgaggcggc
    12961 gcttgacctt gtgccagatc gtcttggcga agcgcagcgg ccgggttacc ttccaggagg
    13021 cggaggtctc cacgcggtac acctcgcgct tcgcggcctc cacctcgcgg gcgtcggcct
    13081 tcagcgcctg cagctcctcc cagagccggt tgcgctcgcg gagcgactcc tcgagcgcgg
    13141 cctcggcgtc gcgcgcgcgt cgctcccagt cggtcgtgat gccggtctcg gtgctcatcg
    13201 cgggctcagc ataggagtca caactcctcc gcgatgcgcg gcgcctcgcg gttgaagatc
    13261 cagaagccga gcacgaacag gccgaaggtg atcgcgatcg ggatcagcag ccggggcgca
    13321 ccgccgatcg cctgcgcggc ggacggggca gtcgggtcga tcacggagtg gcgcacttgc
    13381 tggatcacgg cggcgagcgg gttgcacatc gccaggtgct ggtcggtcgc gttcggcagc
    13441 ttctcgaccg gatagatcac cggcgaggcg tagaagagca actgcaggcc tacctcccag
    13501 atcggccgca cgtcgcggta acggacgtag agcgccgaga gcgccatgcc gatgccggtt
    13561 gcgaacacgc cgaggaaggc gagtaggaac ggcagctcga gccagctcca gcggatgtcc
    13621 actccgctcg cgatcgcgaa gatgaagacg gcgatcaggt tgagcgcgag gttcaggtag
    13681 gcggtgacga ttaccgacag ggggatcacg atgcgcggga actggatctt tcggaccagc
    13741 ccctcacggt caacgaccgc gctcacgcag ccgcccgtcg cctcggctac gaacgtgtag
    13801 agcacgatgc ccgtcagcag tacgactggg tagaactgga cgtcgctgcc gagccgcacg
    13861 acatgcgtga acaccacgta cagcacgccg aacaacagca gcgggcgcac cagctgccac
    13921 aggtagccga gcaccgagcc gaagaacttc agcttgaagt cggtgaaggc gagcgtccag
    13981 gtgaggctca gcaggcggcc gcggccgccc gaaagcgcgg acggcccctt gatcggcctt
    14041 ccgaagtgcg cggcctgggt cgtcacgccg gcgactcctc gggcgcgcgc accggtcgac
    14101 ggatgtcgat ctcgtggggc aggtccacga tcccgccgct gttgcgcgtg cctgtcgaga
    14161 ccatcgacgc ggccgggtcg agctgcgcga ggaccatcgc gccgctgccg gggcgggcca
    14221 cctggaagtt cgcggtgtag cggccaggca cgagctggtt ctcgaaggtc acgcccagga
    14281 ctgcctcgtc gccagcgcgg aactggcccg tgttctcgtc cacccacgtc gttgacgtcg
    14341 cgaacacctt gtgcccgtgc tcgttcgaga gccccaggac gaagaccggg tcgcgcacct
    14401 cggtgttgaa gcgcacgcgc gcccggaatg tggcgggacg gccctgcggc aggtactcgt
    14461 ggcggtcgcc gtgctcttcc tcgaaccagg cgtcgaggat gtcggcgtcg tactcggaga
    14521 ccggctgccc gcccggcgcc gccgcgagcg cgttgccggg gaaattcacg ctgatgtagc
    14581 ggtcggcgac ctcgcccggg tcgccgatcg tgacgagctc gccgcgctcg accagcatcg
    14641 cgcggtggca gaagcgcgtg acggccggca tgtcgtgggt aacgaacagg atcgtcttgc
    14701 gctcttcgcg gaggcggcgg aactcgtcga agcacttctg ctggaaggcg gcgtcgccga
    14761 cggcaagcac ctcgtcgatc agcaggatgt cggcgtccac ctggatcatc accgagaacg
    14821 cgaggcgcac ctgcatgccc gacgagtagt tcttcagctt cagctcctcg aactcgtgga
    14881 gctcggcgaa gtcgagcacg cggtcaacgc gctcacgcgc ctccgccggc gagagcccga
    14941 gcatcactcc gttgaggacg atgttgtcgc gcgcggccag gtccgggttg aaccccactc
    15001 cgagttcgat gaaggtcgcc acgcggccgt tcacgtagat cacgccgctg tcgaccgcgt
    15061 agatgccggc cagacacttg aggagtgtgc tcttgcccga gccgtttcgc ccgacgatgc
    15121 cgaagaactc gcctggctgc acggcgaagc tgacgtcccg cagcgcctgc aggtcgtcgt
    15181 gtgttgcccg cctgaacggg tggagcgccc gctccttgag cgtgtggacg cgctcgtgcg
    15241 ggattctgaa ccgcttcgag acgctgtcga cgaagactgc gggcgcgttg tcctgagagg
    15301 gactcacgca ggcacaatct agcgttggag gcgggccgta ccgcccggta cggtgggtcg
    15361 cgtgacgccc gaagccacgg tcgtagtgcc gacccaccac catggagaac tcctcctgcg
    15421 gtccgttggt tgcgctctgg cccagacagt cggcgatctc gaggtcctga tcgtcgggga
    15481 cggcgcgacc gaggagacgc gtgaggcggc gcttcagctc gaacgctccg atgcgcgcgt
    15541 gcgcttcttc gacaacccga aggggccgcg gctcggcgag gtccatcgcg ccgcggcgct
    15601 cgagcaggcg cagggtcggc tcgtgttcta cctctccgac gacgaccttt ggatgcctga
    15661 ccacgtggag cggatgaccg ccgcgctcga cgctgcgcac gccgacttcg ttcacgcgcc
    15721 accggtttgg gtcatgccgg acggtgagct tctcgtctgg ctcgtggacc tggcgcagcc
    15781 gtggttcagg gagctcgcgc tgcgcgagag ccgcataccg ctctcgtcag gcgctcacac
    15841 gctcgcggcg taccggcggc tgccggtcgg ctggaacacc acgccggaag gccagtacgt
    15901 ggacttcatg atgtggcgcc gcttccttga gcagcgcgac atgaccttcg ccgtcagtcg
    15961 cacgcacacg gtcatgcact tcccaggctc cgtgcgcgag ggcatctcga acgccgagcg
    16021 cgcggatgag ctcgacgact ggcttcccgc cacacgtgat ctcgcggcgc gggccgccta
    16081 cctggagcag ctcgcggcgc acctccacga gcgggcgaac tggctcgact cgcacgccgg
    16141 cgagctcgag agctgggctc agaaccgcag ccaggtcgtg gacctgcgca acgagcagct
    16201 ctcgcaggcc gagcgcgagc gccagcgcct cgaagaggtg atccgagcga tgaagagctc
    16261 gcgccgctgg cgggcgggcg agcggctgcg ccggctcagc cgtccctgag cgcccagtcg
    16321 aacgggatct cgccgctgtg cgggtcgacc cggttcttgt ccggattgac gtggtcgtag
    16381 gggcgcgtgg ggaagttgat gatcatcgcg tcgctgtcgc cccagttctg gtcggcgtgc
    16441 cagacgccgg gcgggatggc cagcaggccg ggagtgcggt ccgtgaagtg gaactccgat
    16501 aaggcgccgt ggctgggcga ctccaacctg ccgtcgagga gcacgactcg gatgttgccg
    16561 cttgccacgt agtaacggtc ggtctggagc ttgtgcatgc cccagccctt gatccggcgc
    16621 ggccggatcg tgaccgagta ggcgtagata acgggctcgc gccagaaggg acgctcgaag
    16681 ttcatgacct ccacgagcga gccgcggtgg tcgtcatgca ccaccagccg ctcgagctca
    16741 acgccgtcta tgccggtact caggcgccgt ccctgcgagt cgaccgttgg cgggtcaggc
    16801 tgccccgccg gggagactgc ttcgctgctg atcagctcgc ccacgctccg atgctacccc
    16861 ccgtccccgc ggtgcgggcc tacaatcccg cctccgtgca ggcaccggga acattggggc
    16921 tgtccgagga gctgcgtcgg tttgtcgagg aggcgccgta cgagcggcgc accatcgtcg
    16981 aattcgtcgg gcgcgccgcg ggctccctcc tgtcgggcgc acgcgtactc gacgtgggcg
    17041 ccggtgacgc gccctaccgc gagctgttcg agcacgtcga ctacgtgacc accgattggt
    17101 cagagtcacc gcacgagcag gcacgtgagg ccgatgtgat cgcctcggcg gagcgcctcc
    17161 cggtcgagga tcagagcttc gacgcactgc tcttcacgca ggttctcgag cacgtcgcgg
    17221 acccgcgtgc ggtgctgggc gaggcgcacc gcgtgctgag ggcgggcggg cggctgttcg
    17281 tgaccgtgcc gcttgtctgg gagctgcacg agctgccgta cgactactgg cgctacacgc
    17341 cggcgagcct cgaaaccctc gccgccggcg ccggtttcac agatgtggac gtgcaggccc
    17401 gaaacgactg cttcaccacg ctcgcccagc tgatgctgaa cgcccggcac gtgatgggac
    17461 gcgcccccga cgggctcgac tcccgtaggg acgaggcggc ggcgctgctc gaggagctcg
    17521 ccgccagggt ggcggagctc gcgccgctcg acgtggaggg catccttccg ctcggcttct
    17581 cgatgaccgc caggcgcgta tgacgccagg cgggcgcagg cccgtcctgt acctcgcgcc
    17641 ttgggtggac tacgggggaa cggacaaggg cacgatcgac tggttccgct ggctcgaccg
    17701 cgaccggtac gctccgtcgc tgatcgccac ccagccgtcg ctcaacaggc gcctgtccga
    17761 ggtgatcccg tacgcggagg aggtgtgggc gctgtccgag ctgatgcgtg gcagcgactt
    17821 ccccggggcg atcttcgatt tcatccacac gcgcgggatc gacgtggtcc acatcatgaa
    17881 ctcgcgcatg ggctacgacc tgctgcccga cctggcggcg ctgcccaagc cgcccgcggt
    17941 cgtggtgcag ctgcacgtgg aggagcagga caagtccggc tacgtgcgct acgtgaccac
    18001 gcgctacggc aacctggtgg acgccttctc ggtcaccagc gagcacctcg ccaatgccgt
    18061 ctccgcctac gacgtgccgc gcgccaagac gcgtgtgatc tacaccggcg tggacgccga
    18121 gcaggagttc aacccggagc gcgtgcgtcc gatcgacggc atcgagcagg ggccggtgca
    18181 catcctctat cccggccggc ttgtgcccca gaaggacccc cttctgatgg tcgaggtgat
    18241 cgcggccctt gcggtcacgg gctcggactt ccgcgtgcac gtggtcggcg agggccagct
    18301 cgagccagcg gtacacgaag cggttcgcga gcggggcctg gagcggttcg tgcgcttcta
    18361 tccgcccacc aacgagatcg ctcgctggtt cgcagcctgc gacctgctgc tgatgacgag
    18421 cgtgttcgaa ggcgtgccgt acgtggtctt cgaggcgatg gcgatggggg tgccgatcgt
    18481 tgcgccggca ctgcccggca acgcagagct gatggacggc gaggcgggcg cgctgatcga
    18541 cccgcgcgac gacgtgggcg cctacgtgcg cgcgctcgcg gagctcgtgg gcgacgacga
    18601 gcgtcgtcgc gagctggggt ggcgcgcgcg acggcgggtg caggagcgct tctcgctcca
    18661 gcgcatggcg gccgaccacg ccgatctcta cgacgagcta cttgccgggc gcgccaagcc
    18721 cgcaaagccg gacgcgccgg tgcgccacga gcttccggag ccgctgcgcc tgcgcggtag
    18781 gccctcgcgc ggccagccgc cagtgagcgt gatcgtgccg tgcttcaacc acggccggta
    18841 cctgccggag tgcctcgcct cgatccgcga gcagagctac gacggcgaga tccagacgat
    18901 cgtggtggac gacggctcca ccgatccgga gaccgtcgag ctgctcaccg agctcgagcg
    18961 cggcgacgac gtcgaggtgc ttcgcatgtc ggagaacagc gggccgagcg cggcgcggaa
    19021 cgcagcgctc gaacgcgcat gcgggcgctt cgtgctgcca gtcgacgccg acaacctgct
    19081 cctccccgag gcgctcgaga aggtggtcgg gcagctgcag aacgccggcg agacagtcgg
    19141 ctacgtctac ccgagccctc agtacttcgg taatcggctc gactggttcc gcgcgcccga
    19201 gttcaacctg caccggctgc tcgacggcaa cttcgccgac acctcgtcac tgctcgaccg
    19261 ccagatcttc gacgcgggct tccgctaccc ggaggttggg cttggtcacg aggactggga
    19321 cttcgcgcta agcctcgccg aacacgaggt gagcggagag cccgcgcggg agcgcacgct
    19381 tctctaccga aagcgcggct tcacccgttc cgatctcgtg gagtacgcgg ccgacgagtt
    19441 caaggagcgc ctgcccgagc gccacggtgc cctttacgag cgcgcggccg atgcgaagtc
    19501 ccgctggtcg cccgcgctgt cgatcgtgac gctgcgtccc gtggatcccg agggggagtc
    19561 gcgcggccgt ctcggcgcgc gcgtgttcag ccaatcgtgc agcgacttcg agctgatcgt
    19621 ccgctccgac acgctctggc ccgacccggg cagcgggccg cacctgcgtc gaatcccggc
    19681 gcaactggct cgcagcgcgt ccgaggctct tcagaccggt tggcacgccg cccgcgggcg
    19741 ctacatgctc gtcacggccg ggaccggcag cgaactgctc gccgaccccg ccttcgtgga
    19801 gaaggttctg cgcgtgttct cctcaaacgc cgagctgcag gcgatcgggc tcacggacgc
    19861 cggcgagaag gggcgcttcc cgctccggct gatcgagccg ggcgaaggga tcgagcccga
    19921 gccccacgcc atcgtgtggc gagcggactg cgacgagctg atgccgcctg ccaccgcact
    19981 ccaggagggc cgcgagctcg cgtcgctcgc cacggcggtc tccggcctgc tcgtgaccca
    20041 gtggcggcac gcaccggcac cgccgcgttt ccgccccgac gcgacgggcg gtgtggcccc
    20101 atcggtccag cggccgtcga aggggcgccc gagcgaggcg gaggaccgcc gctaccggct
    20161 cgaggccgcg cccgcgctgc cgcgcgtgcc cggtggcgtg aggcgctggt cgctcagtcc
    20221 cacgtggatg ccacccggga cgctcccact cgtcaggcat gcgagccgcg acggcttgcg
    20281 ccgcatcgtg accagatcag agctctcgcc ccccggtttc tcgcccgagt tccacctcgg
    20341 catggcacac gcggcggctg tacccgggac ggtgcggctg gaatggagtc ctgccccgga
    20401 ttacgcggcc gtggcgatcg agcggccgga gggcgaccct gaattcacgc tcggctacct
    20461 cgagctcgcc gcgttcccgc tgctccagcc gctgatcctg gcccgccacg gcgcgagcgg
    20521 tcagcacgtg gtcgtggccg gacacggcga tccactgctc gctcacgtgg agtcgcttgg
    20581 cacactcggg ttcgcggagt cctggccggt gtcgccgcgg cgcatcccgc acgcccggct
    20641 cgactacggg cttcttgggc tcgtgcgcac ggtagacctc gatgcgcgga cgcaccgcta
    20701 cggcgtcggc gccgtgccgc cgggcacggt tgcgggcgag ctcggctcgt tgcatatcca
    20761 gccgggcgac cagtcgatcc ccgtgtggct gtcggccgac ggcgtgttct ccaccgaccg
    20821 ctacacgctt ccatcccacg cgcccggccg caagagcgcc gcgaagtggg ccgtcgcgcc
    20881 gctcgcgtgg cgcggcagcg gaccgctcgg tccccgtgtt cgcgccgccg tgcgccgggg
    20941 actgactgcg gtcagcgcgc tctcgggcgg ggcccgtgcg gaggcagagc gccagggtgc
    21001 gccggtcggc tatctgcatc cgcccgacac cgcggtcgag tccgtgccgc tctactcggc
    21061 gtggctccct gtgaccgggg atcagctgct cacgtcacac ccgctcgagg cggccgacat
    21121 ggggtacgtg gagacgacgc tgctcggcag cctcttggcg gcggcgccga tgacagggcg
    21181 gctgggcgtg gaggatgtcg gggtgccatg ggcgtcgcgc ttcggccaca atgcgcggcg
    21241 gtcgtgagcg aggaggccga cattcaggag cgcgtggacg acgcgctggc gggtatccgc
    21301 aacgccgtgc ggcggcagct cggcgtggac cggccgccct ctgtggaggt cgagatcgcg
    21361 gcattgccac gcttctcccc cgaccttccc gaagagctcc atcccgagga gcgcgcgcgc
    21421 ctcgagcgtg aggccgagga gctccagccg tggctccagg gcccgttcct gctgggcggt
    21481 gatctgctcg tgggcggcgc gtggaggaac gaccagcgct ggctcggcct ggcggaccat
    21541 gtgcccaatt tgcaggcgaa gcgcgtgctc gacgtgggca gcaacgcggg ctacgacccc
    21601 ttcatgtgga acctgcgcgg cgccgagtat gtcctcgcct gtgagccgtt tgagttccat
    21661 cgtcaggcgc tcttcctcga gtcggtctat cgcactggga tcgacttcca gcagatcggc
    21721 tggcaggatc tagacgccga gaagcagggc cggttcgacc tgattcattg ccacggcgtg
    21781 ctctaccacg agccgcatcc gatgctgatg ctgaagcgcc tgcgcgagat gctcgcgccg
    21841 ggcgggacga tgctgttcgg ctcgatgatg ctggcgtcgc cagagctgtc cgagtacgcg
    21901 cgcttcgtgc cgggcgcgta ctacggcgac gagacgtggt ggtgggtgcc gggccggctg
    21961 tgcatgcgct ggatgctcga agccgcaggc ttcgtgagcg aggaggagtt cgggatcgct
    22021 aagggcccac tgggcgactt cccgacgatc aacggctact tcagtcttgc gggcggggag
    22081 ccgtcgccgc tcatcgacga tccgctcgcg cttaagccgt gacttcgtcg aggcaggtcg
    22141 tgaccgggcg gtcagcgcgc tccggcggag cgctcgctca ggcggccggc tggctcgcga
    22201 gctggcgctg gacgggcctg gccgcgctca cactcgcctt cgtcgcgtac tactggcaag
    22261 tgatcttcgc cggcaagctg gtctcggccg cggatctcgt gaacctgtat tacccgtacg
    22321 ccgcgaccgc tggcccgggc ttccagcccc ataaccccgc gctcaccgac tccgtcgttt
    22381 cactcgcccc ggccatgacg tacgtacgcg actcggtgtg gtccggtcaa ctgcctctgt
    22441 ggaacccgta caccgagttc ggcatgccct tcttcgcgca aagccagtgg ggcattctgt
    22501 cgccgcttca gctgccgttc ctggcgcctc cgaagctgtt cggggtggag gtagcgcaaa
    22561 cgctgaccgg catgctcaag gtcgcagcgg ccggatcggg cgcctatctg ctggcgcgtg
    22621 cgttgcggtg ctccaagccg gccgccgtgt tcgcgggtat cgcgtggatg cttggcggct
    22681 acacgactgc ctggctttcg atgcccgcga cgagcgccac tgtgatgctg ccgtggatct
    22741 gcctcggggt cgaggcgacg ttgcgatgga ggcggccttg gctcgcggcc gcaggtctag
    22801 cgagcgtgat ggcggtagcg gtgttcggcg gccacccgga ggcgctgctc aatgcgtttt
    22861 acggagtcgt gatctacggc gtggttcggc tcctccagaa tcggcggcac atgcgcggcc
    22921 aggttcgcct gcgcctggcg gcgatcggca cggcgattgt gctcgcgctc ggcctcgttg
    22981 cggtgatcat cgttccagcc gccgaattgc ttcatcacag cgtcgattcg acgctccgcg
    23041 agctgcagta ccgcggcacg gccatcggct ggaacaacgt gatccaggtc tttgacccgc
    23101 atcacttcaa cgatccgaca cacaccggga ctttctacag cgcgtactcg gcgccttggt
    23161 tcgcgggtgt tgtgaccgcg ctcgcggccc ttgttgcact cgtattcagg gatcgacgga
    23221 ttgtgccctt cgtcttgatg tgcgtcttgg cccttgggat tgcaacgaac tcgtggccgt
    23281 ggcagcgcgt cgaggacgct atccccacgc ttggcggcgt tgcgaagagc gctccgctcg
    23341 tgctctgggc gttcggcttg tccatgactg gcgcgctggg cgtagaccgc gtgcttcggt
    23401 tcatccgggg ccggtcaacc gttccgtggc tcgccccggc tgcgggagcg gcgctatgcc
    23461 tggtgctgtt cgtcgagctg tactcgtggg gccatgacta caacccgcga gtcccgcgca
    23521 gcatggtcgg agtcccgcgg cccccgggga tcgatgcgat ccaggcggga cgcggagcgc
    23581 cgcgcaccgt gtttctcggc cacaccatgc cgtttctcct gccgatgcag ttcgagcgcc
    23641 cagacgcccg cggctacgga caacccactc ggcgggacta cgacctcttc atgcggctcg
    23701 cggtcacgga cctgcgcagc gtcttcaacc cgagcgaact cggctatccg atcgccaaca
    23761 ttaagcccca ccaactcgcg gtgctccggg ccgcgaatac tcgttacgcc gcgtggaccg
    23821 acggccggtg gaagttgcag ggctacgacc aggtgagcca gggaccactc aacgtggcgc
    23881 agaccaacaa cgccatgggt cgtgcctatc tggtctcaga ctacgtaacc gaaccgaagc
    23941 tcgaatctgc gatccaaacg gtcgcaggcg agtcgttcga ccctaatcgt caagtggtgc
    24001 tagacgagga tcccgcgggc gccagacctg atccgcttcg ccgtacgccg ggtccgccgc
    24061 cgcaagttac gcattacgag aacaaccacg tggtcgtacg cgcgagactc gcgcgacccg
    24121 gcctccttgt gctctcggat tccgactacc ccggctggag ggcaagcgtg gatggcaaga
    24181 gcgtccgtat gtaccgggcg aatggtctat tcagagcggt gtcgctgccg gccggtgaac
    24241 atgtcgtgca cttcgacttc cggccggcgt ccgtgtacgt cggcgcggcg atctcgctcg
    24301 taagcgtcct gttcattatc ggcctagcgc tgttcggtag gcggcgcgag ctcgagccgg
    24361 ccgaagatcg cggctagcgc cgcgccggcg ggcgccgcga gcagcagcag catcggggct
    24421 ccgtaacgcg gctcgggcaa gtggatgaga aacggcgccg cgagcaccag ggacgcagcc
    24481 agcaccagcc gcgctagcgc ggtgccgcgc aggagccaca gcccgccgag cgccagcacg
    24541 agcatgaacg cctgcacgcc gagcgacagc caatagggaa tcttcgtcgg ccgcccgtac
    24601 tgggcgtggt cgtcgaaagc ctcgagccag agctcgcgct gccagaacgc gctcgccgag
    24661 agctgcttca gcgggcgatg ctccacgttg tcgcggagcg tgtcgaacgc cttgacccgt
    24721 cccgactgcg ccgccgtgat attcagcggc ccgtccgcca tgttcccggg cgtgtagaag
    24781 tggagcgacc gcaagtaggc acccttcggt ccgtccgtgc ggtcaatcgg caagtgcacg
    24841 cccgcagcgg ccacccactc agccccgggg ttgatgccca ccggcgcggg cttgtggagc
    24901 tccgagaagt tgcgggcgat ccagggcgcg tagagcgcga ccacggggag gaccactgcc
    24961 gcggcggcga gcgcacgcac acgcgggcgg tgcaccagga gcggggccgc tacgacgatc
    25021 actccgagcc cgagcaggag gtttgggcgc acgagtgcgg cggcggcggc gaacgcgccc
    25081 gctccggcga accaccaaag ctggcgacga gcgcctcgtc cccagagcag gcagaagcag
    25141 gcggcggcga acaggcttgc ggcgagggcg tccgggagca gcagcgccgg cagcatcacg
    25201 agcggcggaa gcagcgcgag taaggccgcc gtggcgcggc ccgccgtgag gccgcagaag
    25261 cgcgctgcca gcagcgccac gagcagcgcc gcgacgctca cgatcaacgc ctgctcgacg
    25321 cggatcacgt tcgtcccctg cccgagcgag aagttgatcg cgaggagcag ggggtagccg
    25381 ggcgtgcgaa tagcgtctgc ggagctccac cagctcgaga agtcgtgcgc gatcttcgcg
    25441 tactggatct ggtcgccgat gttctccggc ccgtgtgcga ggaccgcgta cacggttcgg
    25501 agcgcgaagc caattgcgct cagccccagg atccacgggt cgagcggcaa ccgcgagcgc
    25561 gtgcgccgcg cctcgctggg gggcggggcg gccggctgct cgaccgtcgt cgccacggtt
    25621 acctcgcggc ctcgaacacg ccgggccgga agcgcttcag cgcggactcg cactgcttgc
    25681 ggctcatgat ctgctcagcc tgcccgctcg cgatgttcac gaagactgcg ttgccgtacg
    25741 acgccggcgg catgccgacc gtcgaggtaa ccgtggtcgc gccgcaggcg agcggggcga
    25801 ggaccgggaa ggccgcgagc gtgcggtcgt ggaagtgcat cttcactgcg ccgttcagat
    25861 cccaactcgc gctgaacacc ggtacgccgg gcgtgtagaa gccgggcgcc tggaacgtgt
    25921 agatcgtcga gccatggggc gggtgcttca gcgtgccgcg caatacggtg atcaccgagt
    25981 tctccatctg gtacgcggtg tcccagcggt cgatgtcgtg ctcgaggtcc tgggcgtagc
    26041 ccacgccgat gacaatggcc actagcgccg ccgcgcctgc agcgagcagg gtcggcgatc
    26101 gccgctcggc acgcatgccg cgggccaaaa gtgtgccggc gataatcgcg gcggagtaca
    26161 cgagcgtgac gagcccgatc gaggcgagca cgttcgtcct gttgccgagg cccgccgtga
    26221 gtggcgagta gtaggcgtcg gcagggatga acatcgcgta gccgacgacg actccccata
    26281 cggccacgcc ggcgaccacg agccagcgcc ggagcatgcg atcgcgcgtc cgagcccaca
    26341 ccacgagtgc cgccacgagg attgccgcaa cgacgccggc cacgagccag cgcgccgggt
    26401 caccgaacgg caccaccgtc cgggcaaaca cggtcacgcc ctggtcggcg atcagcttca
    26461 cgtggtggcg catctgcgcg aacgtctgga tctcgtggtt cccgctgcgc gtgacgagca
    26521 gcagcgtgag tccgatcagc ccgacatccg cgatccagcg cttccagacg acccgcagcg
    26581 ggaagcgcag cgcgtacagc acgatcacgc tgagcgccgc aggagccgtc acctcgtagg
    26641 tcatgacgct cagcaggtac agcgccatcg acgccgcgtg cagcagaagc gcacggcgtc
    26701 cacgcgcctc gaaggcatgc agcgcgacca ccaccccgag caggaaaatc gtgatcgcga
    26761 gcgaggtcat gctcgcggtc gaccacagcc gcgtggagtc agactgcggg aacaggagga
    26821 cgagcaccga gatcagcccc gcgtggagcg gctccatccg gagcgtgcga agcagcaggt
    26881 acagcgaagc cgacatcgcg acggcgagtc cgatcgacca cgcgaggtgc gcagtgaagt
    26941 gagcgccgaa cacctcgtgg gtcagcggca cgtacagcac gagcacgggg cggtaggacg
    27001 tgaagtccca gaagaacttg atcgcgtcga agaagccaga gccgccggtg gctgggtaga
    27061 gcgtggcggc gt
//
LOCUS       DASVXS010000272        10299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1043508, whole genome shotgun sequence.
ACCESSION   DASVXS010000272 DASVXS010000000
VERSION     DASVXS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043508"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1217)
                     /locus_tag="VF752_15365"
     CDS             complement(45..1217)
                     /locus_tag="VF752_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8856973.1"
                     /translation="MVDFTLTDEQKDLREMAHSFAEKEMRPVAWEYDKDGTWPQEILE
                     KAWELGLMNSHIPEEYGGPGLDYFSGTLIEEELGWGCSGIGTSIACNGLATAPIILGG
                     SDETKKKYLGQLTEEPKLASFCLTEPDAGSDVSGMRTTAVRQGDKYVINGSKCFITNG
                     GYADYFTVYAKTDKEAGHRGISAFVVEKDWGVVVDKKEDKLGQRASNTATITFNDVEV
                     PAENLLGEENHGFKLAMMTLDRTRPGVAAMAVGIARAAFEFARDYSKERVQFGVPIAM
                     HQAIQFMIADMATKVEAGRLLTWQSAALLDAGLRNTLASSHAKRFSADSAMEVTTDAV
                     QVYGGYGFIKEYPVEKLMRDAKIMQLYEGTSQIQRLVIAREVLMPRRVEEPATAAA"
     gene            1541..1981
                     /locus_tag="VF752_15370"
     CDS             1541..1981
                     /locus_tag="VF752_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856974.1"
                     /translation="MEGVFPIVVISVCILALVAAFIAFIGSGKLYEGIGKGGLTFDSE
                     ENGLDRAPAPQSAAARAEAEAEIRQMVEAKSARRQARGEEPLDVDAEVRALTGTPETQ
                     VDAALRDEVRQLVIASNERRLRRGEEPLDVEAEVERQLRDLGAG"
     gene            2022..2351
                     /locus_tag="VF752_15375"
     CDS             2022..2351
                     /locus_tag="VF752_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856975.1"
                     /translation="MPRGRRFELDELLIRPGTYFNPQTEVMVVVDDSPSIDSEIFNLE
                     EFEGADWVLVNDELPIDEPRRDELLENFQAIHHGGDGRSAPSDDDLGDDEVSLEDAGL
                     SEDYEEE"
     gene            complement(2379..2681)
                     /locus_tag="VF752_15380"
     CDS             complement(2379..2681)
                     /locus_tag="VF752_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856976.1"
                     /translation="MSDQIVIRNGNGTELELVRLAALDSQKALDGHVLVAEAGGRARA
                     AVAVDNGRAVSDPFYASAPLVDMLRLQAEQVERDVNRPRSGLKLPLPRRQRLAPVR"
     gene            2768..3703
                     /locus_tag="VF752_15385"
     CDS             2768..3703
                     /locus_tag="VF752_15385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8856977.1"
                     /translation="MLDVRRLRVLREVAKHGSFSAAAEALAYTQSAVSQQIAALEREC
                     GAKLVQRGARGIKLTQAGEVLVRHAGVVLAQLADAEAELEALAGLRGGRLRLATFPSA
                     GASLLPVAVSLFRERHPAVELSLIEAEPQDSLPQVKAGEIDIALMYEPHLAGDDALTG
                     LEHRHLLDDPMYVALSVNHPLAKKKRVRMADLSAEPWVNPSSNHCGSHDMLLRATQRA
                     GFEPRIAFQTDDYNAIQGLVAAGVGVALIPALALTAAREDVAVRPLSDARISRGIGVA
                     SLPSAQRSPAAAAMLDMLEAAAERFEDGAPLAEAV"
     gene            3762..4853
                     /gene="cax"
                     /locus_tag="VF752_15390"
     CDS             3762..4853
                     /gene="cax"
                     /locus_tag="VF752_15390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX8856978.1"
                     /translation="MRIRPVYFLGIFIPIAVALELAHAGAVVIFITSALGVIPTAALM
                     GEATEQLAARSGPGIGGLLNVTFGNAPELIIAFFALLKGLQEVVKASIVGSIIGNILL
                     VLGASMLVGGLRRDRQYFNRTAAHAQAAMLLLALVALVMPAIFELIHGGHLPAVSQER
                     VDFSPTVEHLSLAVAIVLIVSYVLGLVFSLRTHRRLFNPYTEETEEEGERPWTMRRAV
                     TVLAIAGVLVGVMSEILVGSISEAASKAGLSEFFIGVIVVAIIGNAAEHWVAVAVAAK
                     DKMDLAVNIAVGSSAQVALFVAPVLVVLSFLFGKGPMSLVFNGYEIGALLFAVLIANF
                     LTQEGESNWFEGVQLLAVYVVLGLVFYYA"
     gene            4864..5685
                     /locus_tag="VF752_15395"
     CDS             4864..5685
                     /locus_tag="VF752_15395"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8856979.1"
                     /translation="MLADVVDCLRCPHCDGGLRLGDGSLRCDSGHSFDVARQGYVYLL
                     PGDVRPGGDSAAMVEAREAFLRARHFAPIAQAVAEAAGQPGGCVADLGAGTGDYLARV
                     LDRAPDAPGLALELSKHALRRAARAHPRIGAVGCDAWRALPLRSRSVSVVLSVFAPRN
                     GEEIARVLSPGGKLVVVTPTRRHLTELITPLGMLSVDERKRERLESKLSPHLAPESEA
                     GLEWTMWLTHEELGWLAGMGPSAFHTDAEELAERIARLPEPMAVTASVTVSVHRS"
     gene            complement(5696..6805)
                     /locus_tag="VF752_15400"
     CDS             complement(5696..6805)
                     /locus_tag="VF752_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856980.1"
                     /translation="MDRLRLRLRLSALLLTLAVGALALGLAACGGGGDSASAKQTLDK
                     AFSTPIKSADLSADLQVKLDGSQQLSGPIQLRVTGPYHSNGQKQLPEMDLEASLSAGG
                     TTLPFGFTTTKNNVWVSVQNQAYELGTQATAQINKQLRQQQKSGQGKTPLSQLGIDPL
                     SWVKDAKEEGDANVAGTSTKHVSGKLDVSKMLEDLNKAVAKAPSGLGATQKPQPLTDK
                     QRSEIEKVVKDPRIDVYVAKSDNTLRRIAANLELSVPENQRAQVGGIKGGTIAFSIEF
                     ANVGSAKAVTPPKNARPLSDLTNQLGGGLGALGSGSGSGSGSGSGSGGSSGSSGSGSS
                     KTPSASDFQKYSDCLRKAGGDTAAQVKCAQALNGG"
     gene            6880..8424
                     /locus_tag="VF752_15405"
     CDS             6880..8424
                     /locus_tag="VF752_15405"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX8856981.1"
                     /translation="MLKRARIAVVGVAALFAFAAQAHAATVYEVKGDRLVRTNDPNVP
                     TADGLPSPAGRCTPPATSPLPAAVHGAAAKTVRGSVRRAFLDGRITREQNDQWRHDYM
                     DARSARKRLSGLRRSELGAVISTLERIAARGQLTSGRMPILFLQLEHNTDFWSHKPIP
                     PAGVIHPDPCAQAFAKKSAPTRISFPGSQLIWQWYPGNGLQIQPLANFGKANGMWTAC
                     DGPPNPKHPCEADKLRQLLDELVAAGARRGTFTAWEYYFPFSGGSPPWISGLTTGTAI
                     QALARGTKLFGDPKYLNTARDALGAFDTSPPTGVLRQVEGGSRYLEYSFAPNLFIVNG
                     FLQGLIGLHDYAAIAQDNHAEALYERGERVAARDVPRSDTGAWSLYSLGGAESDLNYH
                     RLLRDFLNVMCTQTQKSVYCDTAKNFTQDMLTHPKLSFAGPSVARRGRPTAVHFTLDK
                     ISCVQVRITRGGKTAYFKRTSYGHGRRSVTWVPRAAGRYTVQLEAADLGNHHTRASGT
                     ISVRSR"
     gene            8446..9654
                     /locus_tag="VF752_15410"
     CDS             8446..9654
                     /locus_tag="VF752_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX8856982.1"
                     /translation="MAPRTILYTGKGGVGKTSVAAATARRCAEAGLRTIVLSTDPAHS
                     LSDSLEAELGSAPTEVGTNLFGQEVQAQEQMERHWESVQDWLGELLTESGLNQIAAEE
                     LTVPPGMDELFSLLEIKRHHEAGEFDAIVVDCAPTGETLRLLSFPDVASWWLEKVFPW
                     EKRVMAAARPFARTFLDVSLPSEAVFDDVQRLVRNLVAMNEILRDRSATSIRLVMNPD
                     RMVIKESMRTFTYLNLYGYLTDAVLVNRLFPDELEGSYFDSWREAQREHMELVRSAFS
                     PVPILSAPYFEREVVGAEMLDRLSAELFGELDPGAILHNELTQELSTQNGSATLRLPI
                     PFAEKGDIDLKKIGLEMIVRVGPQKRTIILPPAMAAYRPRGARFEDGALEVSFEKQDG
                     GVEAAAEHAE"
     gene            9617..9946
                     /locus_tag="VF752_15415"
     CDS             9617..9946
                     /locus_tag="VF752_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856983.1"
                     /translation="MAESRQQQSTQSEHAGPAQPPRWDEVDWERFVAGNGSGAPPPRL
                     GRGPDLAPLFLILDALRAGLPRELRDQFNTLVRELLLTLRALIDWYLERLDGGRREPE
                     VEDIPID"
BASE COUNT         1528 a         3536 c         3557 g         1678 t
ORIGIN      
        1 ccagcgggcg gcctctcgca aacgcatccg tcgagcccgg ctcgctacgc ggcggccgtc
       61 gccggctcct ccacgcggcg gggcatgagg acctcgcgcg cgatgacgag acgctggatc
      121 tgcgacgtgc cctcgtagag ctgcatgatc ttcgcgtcgc gcatcagctt ctcgaccggg
      181 tactccttga tgaagccgta cccgccgtag acctgcacgg cgtccgtggt cacctccatc
      241 gcgctgtcgg cggagaagcg cttcgcgtgc gacgaggcga gcgtgttgcg cagtcccgcg
      301 tcgagcaggg cggccgactg ccatgtgagc aggcgccccg cctcaacctt ggtcgccatg
      361 tcggcgatca tgaactggat cgcctggtgc atcgcgatcg gcacgccgaa ctgcacgcgc
      421 tccttcgagt agtcgcgggc gaactcgaac gcggcgcgcg cgatgccgac ggccatggcg
      481 gccacgccgg ggcgcgtgcg gtcgagggtc atcatcgcga gcttgaagcc gtggttctcc
      541 tcgcccagca ggttctcggc gggaacctcg acgtcgttga acgtgatcgt ggcggtgttg
      601 gaggcgcgct gtccgagctt gtcctccttc ttgtcgacga cgacgcccca gtccttctcg
      661 accacgaagg ccgagatgcc gcggtgaccc gcctccttgt cggtcttggc gtagaccgtg
      721 aagtagtcgg cgtagccgcc gttcgtgatg aagcacttgc tgccgttgat cacgtacttg
      781 tcgccctggc gcacggcggt ggtgcgcatg cccgacacgt cggagccggc gtccggctcg
      841 gtgaggcaga acgacgcgag cttcggctcc tcggtgagct ggccgaggta cttcttcttc
      901 gtctcgtcgg agccgccgag gatgatcggc gcggtcgcga gaccgttaca ggcgatcgag
      961 gtgccgatgc ccgagcaacc ccagccgagc tcctcctcga tcagcgtgcc gctgaagtag
     1021 tcgagccccg ggccgccgta ctcctcggga atgtgggagt tcatcaggcc gagctcccag
     1081 gccttctcga ggatctcctg gggccaggtg ccgtccttgt cgtactccca ggcgactgga
     1141 cgcatctcct tctcggcgaa ggaatgcgcc atctcgcgca ggtccttctg ctcgtcggta
     1201 agcgtgaaat cgaccacgga tgctccttgt gctgcggttc gtttcgggcg cgctacgcgc
     1261 cctgcaaaga ctcgattgat tcgtcagtca atgactgtct caatggtacc cgaccaccgg
     1321 tcgccggaaa accggtaacc gtccttgcac cccgcaacta ccgcatccgg gctagtggcg
     1381 aagtcacccg aaaagcgctc gtagagcgaa atagaggccg atcgccatcg ccacgagtgt
     1441 gaacacgagc agcccaacct ggtccgggta gagggcgggt cgaccgggtg cgaacgacct
     1501 acggcgtagg cgacgccaca tcgcagtcaa actgtacggg gtggagggcg tctttccgat
     1561 agtcgtgatc tccgtgtgca tcctcgcgct ggtggccgcg ttcatcgcgt tcatcggctc
     1621 ggggaagctg tacgagggga tcggcaaggg aggcctcacc ttcgacagcg aggagaacgg
     1681 actcgaccgt gcgccggcgc ctcagagcgc cgccgcccgt gccgaggccg aggccgagat
     1741 ccgccagatg gtggaggcca agtccgcgcg gaggcaggcg cgcggcgaag agccgctgga
     1801 cgtggacgct gaggtcaggg cgctcaccgg cacgcccgag acgcaggtcg atgcggcgct
     1861 cagggacgag gttcggcagc tcgtgatcgc gagcaacgag cggcgcctgc ggcgcggcga
     1921 ggagccgctc gacgtggaag ccgaggtcga gcgtcagctc cgagacctgg gcgccggcta
     1981 gagcggtcgc ccagcgtccc aatcggatat acctacgcca aatgccacgc ggacgacgct
     2041 ttgagctgga cgagctgctg atccggccgg ggacttactt caacccccag accgaggtca
     2101 tggtcgtggt cgacgactcg ccgtcgatcg actccgagat cttcaacctc gaggagttcg
     2161 agggggccga ctgggtgctc gtgaacgacg agcttccgat cgacgagccc cgtcgcgacg
     2221 aactgctcga gaacttccaa gccattcacc acggtggcga cggccgctcg gccccgtccg
     2281 acgacgacct gggagacgac gaggtctccc tggaagacgc cggcctcagc gaggactacg
     2341 aggaggagta gtccgcgccg aggccggtcc tgcgcccgtc agcgcacggg cgcgagccgc
     2401 tgccgacggg ggagcggcag cttgaggccc gagcgcgggc gattgacgtc gcgctcgacc
     2461 tgctccgctt ggagccggag catgtccacg aggggcgcgc tggcgtagaa gggatctgaa
     2521 accgctcggc cgttgtcgac cgctacggcg gcgcgggcac gcccgcctgc ctcggccacc
     2581 aggacgtggc cgtcgagcgc cttctggctg tcgagcgccg cgagccgtac cagctcgagc
     2641 tccgttccgt tgccgtttct tatgacgatc tggtcgctca tcgcgttctc ctttcggatc
     2701 accaaggtga cagtcgagcg tcataaaggc aaatgaaggg ttacgggaga tgcgataaga
     2761 tgcgctaatg ctcgacgtgc gtcgcctccg agtgctccgc gaggtcgcca agcacgggtc
     2821 gttctccgcc gcggcggagg ctctcgctta cacgcagtcg gccgtgtcgc agcagatcgc
     2881 ggcgctcgag cgcgagtgcg gcgcgaagct cgtgcagcgc ggcgcgcgcg gcataaagct
     2941 cacccaggcg ggcgaggtgc tcgtgcgcca cgcgggcgtt gtgctcgcac agctcgccga
     3001 cgccgaggcc gagctcgaag cgctcgccgg cctgcgcggg ggccgcctgc gcctcgcgac
     3061 cttcccgagc gccggcgcct cgctcctccc cgtcgccgtc tcactcttcc gcgagcgcca
     3121 ccccgccgtc gagctgtcgc tgatcgaggc cgagccgcag gattctctgc cccaggtgaa
     3181 ggccggggag atcgacatcg cgctcatgta cgagccgcat ctcgcgggcg acgacgccct
     3241 gaccggcctc gagcacaggc acctgctcga cgacccgatg tacgtggcgc tatcggtgaa
     3301 ccacccgctc gccaagaaga agcgcgtgcg catggccgac ctctccgcgg agccgtgggt
     3361 gaacccgtcg tcgaaccatt gcggctccca cgacatgctg ctgcgagcga cccagcgcgc
     3421 gggcttcgag ccgcggatcg cgttccagac cgacgactac aacgcgatcc aggggctcgt
     3481 ggcggccgga gtgggcgtcg ccctcatccc ggcgctcgcg ctcaccgcgg cccgcgagga
     3541 cgtggcggtg cgaccgctct cggacgcgcg catctcgcgc ggcatcggcg tcgcgtcgct
     3601 tccctccgcc cagcgctccc ccgccgcagc cgccatgctc gacatgctcg aagccgccgc
     3661 cgagcgcttc gaggacggcg ctccgctcgc cgaagcggtg tagcagcccc agctcgcgcc
     3721 gccggccggg ccgcgagccg ctcccatagg cttgcgccct cgtgcggatc cgtccggtct
     3781 acttcctggg aatcttcatc ccgatcgcgg tggcgctcga gctggcccac gccggcgcgg
     3841 ttgtcatctt catcacttcg gcgctcggag tgatccccac cgctgcgttg atgggcgagg
     3901 ccacggagca gctcgcggcg cgctcggggc ccggcatcgg tgggctgctg aacgtgacgt
     3961 tcggcaacgc gcccgagctg atcatcgcct tcttcgcgct tctgaaggga ctgcaggagg
     4021 tggtcaaggc gtcgatcgtc ggctcgatca tcgggaacat cctgctcgtg ctcggggcat
     4081 cgatgctcgt cggcgggctc aggcgcgacc ggcagtactt caaccgcacg gcggcgcacg
     4141 cgcaggccgc gatgctgttg ctcgcgctcg tagccctcgt gatgccggcc atcttcgagc
     4201 tgatccacgg cggtcacctt ccggcggtgt cgcaggagcg ggtggacttc agcccgaccg
     4261 tcgagcacct ctcgctcgcg gtagcgatcg tgctcatcgt cagctacgtg ctcggcctcg
     4321 tcttctcgct gaggacgcac cgacggctgt tcaaccccta cacggaggaa acggaggagg
     4381 agggcgagcg tccgtggacg atgcggcgag cggtgacggt tctcgccatc gccggtgtgc
     4441 tcgtgggagt gatgagcgag atcctcgtcg gctcgatcag cgaggccgcc tcgaaggcgg
     4501 ggctgtccga gttcttcatc ggggtgatcg tcgtcgccat catcggcaac gcggcggagc
     4561 actgggtggc ggtcgcggtg gcagccaagg acaagatgga tctcgcggtg aacatcgccg
     4621 tcggatcgag cgcgcaggtg gcgctgttcg tagcgcccgt gctcgtggtg ctctcgttcc
     4681 tgtttggcaa gggaccgatg tcgctcgtct tcaacggtta cgagatcggc gcgctgctgt
     4741 tcgcggtgct gatcgcgaac ttcctcacgc aggagggcga gtcgaactgg ttcgagggcg
     4801 tgcagctgct cgcggtgtac gtggtgctcg ggctggtctt ctactacgcc tgagcgggcc
     4861 gccgtgctgg ccgacgtagt cgactgtctg cgatgccctc actgcgacgg cggcctgcgg
     4921 ctcggcgacg gctcgctgcg ctgcgactcc ggtcacagct tcgacgtcgc tcgccagggc
     4981 tacgtctatc tactccccgg tgatgtccgg cccggcggcg acagcgctgc gatggtggag
     5041 gcccgcgagg ccttcctgcg cgcgcgtcac ttcgcaccga tcgcccaggc ggtggcggaa
     5101 gcggccggcc agccgggcgg ctgcgtggcc gacctcggcg ccggcacggg cgactacctc
     5161 gcgcgcgtgc tcgatcgagc tccggatgcg cccgggctcg cgctcgagct gtcgaagcat
     5221 gcgctccggc gtgccgcgcg ggcgcatccg cggatcggcg cggtgggctg cgacgcttgg
     5281 cgcgcgctgc cgctgcgctc gcggtcggta tccgtcgtgc tcagcgtgtt cgcaccccgc
     5341 aacggtgagg agatcgcacg ggtgctcagc ccgggcggga agctcgtcgt ggtcacgccc
     5401 acgcgccggc acctgacgga gctcatcact ccgctcggca tgctcagcgt ggacgagcga
     5461 aagcgcgagc gtctcgaaag caagctctcg ccgcatctcg cgccggagtc cgaagctggc
     5521 ctcgagtgga ccatgtggct cacgcacgag gagctcggct ggctggccgg gatgggaccg
     5581 agcgcgtttc acacggacgc cgaggagcta gccgagcgga tcgcgcggct gcccgagccg
     5641 atggccgtga ccgcgtcggt cacggtctcg gtgcaccgga gctagcggga gccgctcagc
     5701 cgccgttcag ggcttgcgcg cacttgacct gcgcggccgt gtcgcctccg gccttgcgca
     5761 ggcagtcgct gtacttctgg aagtcgctgg cgctcggggt cttcgacgag ccggatccgc
     5821 tggacccgct cgagccgccg gagccggagc ccgaccccga ccccgacccg ctgccggagc
     5881 cgagggcgcc gaggccgcca cccagctggt tggtgaggtc ggagagcggc cgcgcgttct
     5941 tcggcggagt gaccgccttg gcgctgccca cgttcgcgaa ctcgatcgag aacgcgatcg
     6001 tgccgccctt gatccccccg acctgcgcgc gctggttctc aggcacgctc agctcgaggt
     6061 tcgccgcgat gcggcgcagc gtgttgtcgg acttcgccac gtacacgtcg atgcgcgggt
     6121 ccttgacgac cttctcgatc tcggagcgct gcttgtcggt gagcggctgc ggcttctgcg
     6181 tcgcaccgag accgctgggc gccttcgcga ccgccttgtt cagatcctcg agcatcttcg
     6241 acacgtcgag cttgccggag acgtgctttg tcgacgtgcc ggccacgttc gcgtcgccct
     6301 cctccttggc gtccttcacc cagctgagcg ggtcgatgcc gagctgcgag agcggtgtct
     6361 tgccctgtcc cgacttctgt tgctgccgga gctgcttgtt gatctgcgcg gtcgcctgcg
     6421 tgccgagctc gtaggcctgg ttctgcaccg acacccagac gttgttcttg gtggtcgtga
     6481 agccgaaggg aagggtcgtg ccgccagccg acaggctcgc ctcgaggtcc atctcgggga
     6541 gctgtttctg gccgttgctg tggtacggcc ccgtcacccg cagctggatc ggcccggaca
     6601 gctgctgcga gccgtcgagc ttcacctgca ggtcggccga gaggtccgcg ctcttgatcg
     6661 gcgtgctgaa cgccttgtcg agcgtctgct tggcgctcgc cgagtcgccg ccgcctccgc
     6721 aggcggcaag accgagcgcg agcgcgccga ctgcgagcgt gagcaggagc gcggatagtc
     6781 gaagtcgaag tcgaagtcgg tccaccaaga ccaggcaggg tacccgttcc gatcgcggct
     6841 ctccgcaact cgaggcgtta agaaatgttg gttcggcaca tgctgaagag ggctcgaatc
     6901 gccgttgtgg gcgtcgccgc gctgttcgcc ttcgccgcgc aggcacacgc cgcgacggtc
     6961 tacgaggtga agggcgatcg gctcgtgcgc acgaatgacc cgaacgtccc gaccgcggac
     7021 ggccttccct cccccgccgg tcgctgcacg ccgccggcga cgtctccgct tccggccgcc
     7081 gtgcacgggg cggcggccaa gaccgttcgg ggttcggtcc gccgggcgtt cctcgacgga
     7141 cggatcacgc gcgagcagaa cgaccagtgg cgccacgact acatggacgc ccgctccgcg
     7201 cgcaagcgct tgtcggggct gcgccgcagc gagctcgggg cggtcatctc gacgctcgag
     7261 cggatcgcgg cgcgcgggca gctcacgtcg ggccgcatgc cgatcctctt cctacagctc
     7321 gagcacaaca ccgacttctg gagccacaag ccgatcccgc cggctggggt aatccatccc
     7381 gatccgtgcg ctcaggcgtt cgccaagaag tcggcgccca cgcggatcag cttccccggc
     7441 agccagctga tctggcagtg gtatccgggc aacggattgc agattcagcc gctcgccaac
     7501 ttcggcaagg cgaacggcat gtggaccgcc tgcgacggcc cgccgaaccc gaagcacccg
     7561 tgcgaggccg acaagctgcg tcagctgctc gacgagctcg tggccgccgg cgcccgccgc
     7621 ggcaccttca ccgcctggga gtactacttc cccttcagcg gcggatcgcc gccgtggatc
     7681 agcgggctca cgaccggcac ggcgatccag gcgctcgcgc gcggcacgaa gttgttcggc
     7741 gatccgaagt acctcaacac cgcgcgcgac gcgctcggcg cgttcgatac ctcgccaccg
     7801 accggcgtgc tccgccaggt ggagggcggc agccgctacc tcgagtactc gttcgcgccc
     7861 aacctgttca tcgtgaacgg cttcctccag gggctcatcg ggctgcacga ctacgccgcg
     7921 atcgcgcagg acaaccacgc ggaggcgctc tacgagcgcg gcgagcgcgt ggcggcgcgc
     7981 gacgtgccgc gttccgacac cggcgcgtgg tcgctgtact cgctcggcgg cgccgagtcc
     8041 gatctcaact accaccggct gctgcgcgac ttcctgaacg tgatgtgcac gcagacgcag
     8101 aagtcggtct actgcgacac ggccaagaac ttcacgcagg acatgctcac tcacccgaag
     8161 ctgagcttcg ccggcccgtc tgttgcgcgc cgcgggcgtc cgacggcggt ccacttcacc
     8221 ctcgacaaga tctcgtgcgt gcaggtgcgc atcacgcgcg gcggcaagac cgcttacttc
     8281 aagcgcacct cctacgggca cgggaggcgc tcggtcacct gggtgccgcg cgccgctggg
     8341 cgttacacgg tgcagctcga agccgccgac ctgggcaacc accacacgcg cgcgagcggc
     8401 actatcagcg tccggtcccg ctagcgcggg tacgatcggc gcttcatggc gccgcgcacg
     8461 atcctctaca ccggcaaagg cggagtcggc aagaccagcg tggccgcggc cacagcgcgc
     8521 cgctgcgccg aagccggcct gcgcacgatc gtgctgtcca ccgacccggc gcacagcctg
     8581 tcggactcgc tggaggcgga gctcgggagc gcgccgaccg aggtcgggac aaacctgttc
     8641 ggccaggagg tccaggcgca ggagcagatg gagcgccact gggagtcggt gcaggactgg
     8701 ctcggcgagc tgctcacgga gagcgggctg aaccagatcg ccgccgagga gctcaccgtg
     8761 ccgcccggca tggacgagct gttctcgctg ctcgagatca agcgccacca cgaggcgggc
     8821 gagttcgacg cgatcgtggt ggattgcgcg cccacaggcg agacgctgcg gctcttgtcg
     8881 ttccccgacg tcgcgagctg gtggctcgag aaggtctttc cgtgggagaa gcgcgtcatg
     8941 gcggcggccc gcccgttcgc gcgcacgttt ctcgacgtgt cgctgccgag cgaggcggtg
     9001 ttcgacgacg tgcagcggct cgtgcgcaac ctcgtcgcga tgaacgagat cctccgcgac
     9061 cgctcggcca cctcgattcg gcttgtgatg aacccggacc gcatggtgat caaggagtcg
     9121 atgcggacgt tcacctatct gaacctctac ggctacctga ccgacgcggt gctcgtgaac
     9181 cgcctcttcc ccgacgagct cgagggcagc tatttcgaca gctggcgcga ggcgcagcgc
     9241 gagcacatgg agctcgtgcg ctcggcgttc tcgccggtgc cgatcctgtc ggcgccttac
     9301 ttcgagcgcg aggtggtggg agcggagatg ctcgaccgcc tgtcggcgga gctcttcggc
     9361 gagctcgacc ccggcgcgat cctgcacaac gagctgacgc aggagctaag cacgcagaac
     9421 gggtcggcca cgctgcggct gccgatcccg ttcgcggaga agggcgacat cgacctgaag
     9481 aagatcggcc tcgagatgat cgtgcgcgtc ggcccgcaga agcgaactat catcctgccg
     9541 cccgcgatgg cggcctaccg gccgcgcggg gcgcggttcg aggacggcgc actcgaggtc
     9601 tcgttcgaga agcaagatgg cggagtcgag gcagcagcag agcacgcaga gtgagcacgc
     9661 cggcccggct cagcctccgc gctgggacga ggtcgactgg gagcgcttcg ttgccggcaa
     9721 cggctcgggc gcgccgccgc cccggcttgg ccgcggcccg gacctcgcgc cgctgttcct
     9781 gatcctcgac gcgctgcgcg cggggctccc gcgcgagctg cgcgatcagt tcaacacgct
     9841 cgtgcgtgag ctgctgctca cgttgcgggc cctgatcgac tggtacctcg agcggttgga
     9901 tggcgggcgg cgggagcctg aggtcgagga cataccgatc gattgattcg gcgacgccca
     9961 gggcgcgggg cgtctacagc agcaataaca gggtggtccg gaggggtggg tgactttgag
    10021 gtcgcatacc gaccctaaag tcacccacgc ctagagcggg gtagcccgtg tgcggtagag
    10081 cttgtcgcgt ggatgccgca aagcgagcgc ggtgcccccg cccgcgagct gacccgttat
    10141 tgcggctgta agcgcggcgc accacgtgcc cgcaccacgg cagaccccgc gcgctactgc
    10201 gagatatcga tgtcccgcag cgacgcctcg tccggcaact tgtccaggac ctcgcgcacg
    10261 cgcgcgtgca cgtcgtgctc ctcgtgcccg aagtcgtcc
//
LOCUS       DASVXS010000273         5089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_831606, whole genome shotgun sequence.
ACCESSION   DASVXS010000273 DASVXS010000000
VERSION     DASVXS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831606"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..795
                     /locus_tag="VF752_15420"
     CDS             172..795
                     /locus_tag="VF752_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8856984.1"
                     /translation="MTTPLDAIADPVRLAMLRHLSEARRATLPELASAAGVHQNTARA
                     HVAALEDAGLLRSEQQAASGRGRPAVDFVLSDGWSMSATDFLALAELLASALARFDVP
                     DEELREMGSDWGRYLVGRPGRHDTVPELIEALERLGWHASYSGGKLKLRGCPCPIVSP
                     DRPHVICDLADGVTDGVCAASGAKLRTVARDHDPELRTCALTLEETS"
     gene            868..1323
                     /locus_tag="VF752_15425"
     CDS             868..1323
                     /locus_tag="VF752_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856985.1"
                     /translation="MAGSLPPGPDRSPLRLTVEYVRRPFALLEECERSYGDPFMLRFP
                     QPPPFIVFSSPAAVREVFRGDPDILRSGQANRIFEAGLGRRSILVLYRPAFAWRRVST
                     SCTGAATSIRIRRPSGPSDSWMAARTGSRGSPSEAAPGAASARRLPRSR"
     gene            complement(1445..2299)
                     /locus_tag="VF752_15430"
     CDS             complement(1445..2299)
                     /locus_tag="VF752_15430"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX8856986.1"
                     /translation="MASARPERRVRRTRRRRRRSDLVGRILAAIPAIAFAVFIVAEGG
                     LVFVIGLLILGALGLAELYNMMSRARPVHPAGYIALLALLLAAHYGDQYQVLLVLWAS
                     LPLTFLLGVMRPRRENISWGIAVTMLGIVWIGVAFAHAVFLRDLEHGGGLVLDVLIGT
                     FVGDTAAYLGGRAWGRRPLAPTISPNKTLEGLLVGVLFGGLAFWLFAVAYQDWFVGTD
                     ALIMGFAVAAAAPFGDLFESLLKRDLAVKDTGTLFGAHGGVLDRLDAVFFTAVVGYYV
                     ARAVGFTA"
     gene            complement(2302..2964)
                     /gene="uppS"
                     /locus_tag="VF752_15435"
     CDS             complement(2302..2964)
                     /gene="uppS"
                     /locus_tag="VF752_15435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX8856987.1"
                     /translation="MSARYVAIIMDGNARWARQRDLPVLEGHRQGAQALKQTVRNAVQ
                     LDIEELTVYAFSTENWTRPDWEVQGLMSMFAELIESETPELDEEGVRMVFIGRRDRIS
                     DELRERMIWAEEKTGGNDRMTLYVAFDYGGRAEIVDAARRFTDGGEEDFARLLYAPEM
                     NDPDLLIRTSGERRLSNYLLWQSAYSELVFSDALWPDFDKDELQAALDEYAARQRRFG
                     AR"
     gene            complement(2991..3557)
                     /gene="frr"
                     /locus_tag="VF752_15440"
     CDS             complement(2991..3557)
                     /gene="frr"
                     /locus_tag="VF752_15440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX8856988.1"
                     /translation="MSELIEELLDDAGSRMAKSVEATRNEFATVRTGRASPHLLDRVA
                     VDYYGAQTPLKQLASISASEARLLTVTPYDKSSIRSIEKSIMESDLGLTPSNDGNVIR
                     LTIPELTEERRRELVKIVRAIAEEGRVAIRNIRRDIMHTLRELKQEGEVGSDEEHRAE
                     TELQKLTDERISEIDSFLAGKEEEILEV"
     gene            complement(3554..4303)
                     /gene="pyrH"
                     /locus_tag="VF752_15445"
     CDS             complement(3554..4303)
                     /gene="pyrH"
                     /locus_tag="VF752_15445"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX8856989.1"
                     /translation="MAGEGGRDGAVAAPAFRRVLLKLSGEALMGDQPYGADADRIQAI
                     ARQIQLTVERGVEMAVVVGGGNIYRGLQGAAAGMDRATGDYMGMLATVLNALALQDAL
                     EKLGAHTRVQSAITISEVAEPYIRRRAIRHLEKGRIVIFAAGTGNPFFTTDTAAALRA
                     LEIHAEAMLMAKNGVEGVFDADPRTTPDAHFLPEITHHQAIERRLGVMDSTALSLCMD
                     NSLPIYVFNIDDERNIERIVSGERVGTVVST"
     gene            complement(4311..4919)
                     /gene="tsf"
                     /locus_tag="VF752_15450"
     CDS             complement(4311..4919)
                     /gene="tsf"
                     /locus_tag="VF752_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX8856990.1"
                     /translation="MSTAEIKASDVKALRDRTGAGMMDCKAALQEAGGDVDKAVEILR
                     VKGQAQAAKRGGRTASEGLVESYIHAGGKIGVLVEVNSETDFVARNDDFREFVREVAL
                     HIAAAAPQYVSEDEVPDEIRQAEQRIYEEQAQDKPEQARPKIVEGRWRKWLEENVLLN
                     QEHIRRDEHDGKTIEELRAEIAANTGENIVIRRFTRYVVGEE"
BASE COUNT          715 a         1821 c         1721 g          832 t
ORIGIN      
        1 tggcgctgat cgccaccggc gcagtccgat ggcggcggcg gggccctcat gttcgagccg
       61 cggcaccgcc ggcagcgtcg cccgaggacc acgcgcgcgt ctcctcggag cttgcccgtt
      121 tcgagccgta gagcggctcc atccggctgc acgccgtact ctgaggttgg gatgactaca
      181 ccgctcgatg cgatagcgga ccctgtgcgc ctggccatgc tcaggcacct gtccgaggcg
      241 cgccgcgcga cgctgccgga gctcgcgtcc gccgcgggcg ttcaccagaa cacggcgcga
      301 gcgcacgtag ccgcgctcga ggacgcgggc ctcctacggt ccgagcagca ggcagccagc
      361 ggtcgcggac ggccggcggt tgacttcgtg ctcagcgacg gatggtcgat gtccgccacg
      421 gacttcctcg ctctcgccga gctcctcgcg tctgccctcg ccaggttcga cgtcccagac
      481 gaggagctcc gagagatggg gagcgactgg ggccggtacc tcgtggggcg gccgggacgc
      541 cacgacaccg tcccagagct catagaggcg ctcgagcggc tcggctggca tgcgtcctac
      601 tccggcggca agctgaagct cagagggtgt ccgtgcccga tcgtctcacc cgatcggccg
      661 cacgtcatat gcgacctggc ggacggcgtt accgacggcg tctgcgccgc aagcggcgcc
      721 aagctcagga cggttgcacg cgaccacgac cccgagcttc gcacctgcgc gctcacgctc
      781 gaagagacgt cctgaatcac gcgagccgga acgcgtgacc gacgccactt tgttgactgc
      841 cgcggaccgg ggcgagacgc tgcggtcatg gcgggctcgc tgccgccggg accggatcgc
      901 tcgccgcttc ggctgaccgt cgagtacgtc cggcggccgt tcgcactgct cgaggagtgc
      961 gaacgaagtt acggcgatcc gttcatgctc aggttccccc agccgccgcc gttcatcgtc
     1021 ttctcgagtc ccgcagcggt gcgcgaggtc ttccgcggtg acccggacat cctccgctcg
     1081 ggccaggcga accggatctt cgaggcgggg ctcgggcgcc gctccatcct cgtcctctac
     1141 cggccggcgt tcgcgtggcg ccgagtgtcc acctcgtgca ccggcgccgc gacgtctatc
     1201 cggatccgga gaccttcagg cccgagcgat tcgtggatgg cggcccggac gggttctcgt
     1261 ggatcccctt cggaggcggc acccggcgct gcctcggcgc ggcgcttgcc acgttcgaga
     1321 tgaaagcgat cctccaggcg gccgcgcgtg cgggtcggcc cgcgccggcc cgagatggag
     1381 agggcgtggg tcggcgtggg ttgacgcttg caccggcccg cggcgggcga ctcgtctggg
     1441 agccctacgc ggtgaagccg accgcgcggg caacgtagta gccgaccacc gcagtgaaga
     1501 agacggcgtc gagacggtcg agcaccccgc cgtgcgcgcc gaacagcgtg cccgtgtcct
     1561 tcacggcgag gtcgcgcttc agcagcgatt cgaacaggtc gccgaatgga gcagccgcgg
     1621 ccaccgcgaa gcccatgatc agcgcgtccg tgcccacgaa ccagtcctgg taggccaccg
     1681 cgaagagcca gaacgccagg ccgccgaaga ggactcccac gagcagcccc tccagcgtct
     1741 tgttcggcga gatcgtcgga gcgagcggcc gccggcccca cgcgcggcct ccgagatacg
     1801 ccgcggtgtc gccgacgaag gtgccgatga gcacgtcgag cacgagcccg ccgccgtgct
     1861 cgaggtcacg caggaacact gcgtgggcaa acgcgacccc gatccacacg atcccgagca
     1921 tcgtcacggc gatgccccac gagatgttct cgcgccgcgg gcgcatcacg ccgagcagga
     1981 acgtgagcgg cagcgacgcc cagagcacca gcagcacctg gtactggtcg ccgtagtgcg
     2041 cggcgaggag cagcgcgagg agcgcgatgt agcccgccgg gtgaaccggc cgggcgcggg
     2101 acatcatgtt gtagagctcg gcgagcccga gcgcgccgag gatcagcagg ccgatcacga
     2161 agacgaggcc gccctcggcg acgatgaaga ccgcgaacgc gatcgccggg atcgcggcga
     2221 ggatgcggcc gacgagatcc gagcgccggc ggcgccggcg ggtgcggcgg accctgcgct
     2281 caggccgcgc cgacgccacg gtcagcgcgc cccgaagcgg cgctgtcggg ccgcgtactc
     2341 gtcgagggcc gcctgcaact cgtccttgtc gaagtctggc cagagagcgt ccgagaacac
     2401 gagctcggag tacgcgctct gccagagcag gtagttcgag agccggcgct ccccgctcgt
     2461 gcgaatgagc aggtcgggat cgttcatctc gggcgcgtag agcaggcggg cgaagtcctc
     2521 ctcgccgccg tccgtgaagc gccgcgccgc gtccacgatc tccgcgcgac cgccatagtc
     2581 gaacgcaacg taaagcgtca tccggtcgtt cccaccggtc ttctcctccg cccagatcat
     2641 cctctcgcgc agctcgtccg agatgcggtc gcgcctgccg atgaagacca tccgcacgcc
     2701 ctcctcgtcg agctccggcg tctccgactc gatcagctcg gcgaacatcg acatcaggcc
     2761 ctggacctcc cagtcgggtc ttgtccagtt ctccgtggag aatgcgtaca cggtcagctc
     2821 ctcgatgtcg agctgcaccg cgtttcgaac ggtctgcttc agcgcctggg cgccctgccg
     2881 gtgaccctcg agcacgggca ggtcgcgctg ccgggcccag cgcgcgttgc cgtccatgat
     2941 gatggcgacg tagcgcgctg acatggaacc gggggcgggc ggcagccgcc ctatacctcg
     3001 aggatctcct cttccttgcc tgcgaggaag ctgtcgatct cggaaatgcg ctcgtcggtg
     3061 agcttctgca gctcggtctc ggcgcggtgc tcctcgtcgg agccgacctc cccctcctgc
     3121 ttgagctcgc gcagcgtgtg catgatgtcg cgccggatgt tgcggatcgc gacgcgtccc
     3181 tcctccgcga tcgcgcgcac gatcttcacc agctcgcgcc ggcgctcctc ggtcagctcg
     3241 gggatcgtga ggcggatcac gttgccgtcg ttcgacggcg tcaggccgag gtcggactcc
     3301 atgatcgact tctcgatcga gcggatcgag gacttgtcgt agggggtcac ggtgagcagc
     3361 cgcgcctcgc tcgccgagat actcgcgagc tgcttcagcg gcgtctgcgc gccgtagtag
     3421 tcgacggcca cgcggtcgag caggtgcggg gacgcgcggc cggtgcgaac ggttgcgaat
     3481 tcgttgcgcg ttgcctcgac ggacttggcc attcggctgc ccgcgtcgtc gagtagctcc
     3541 tcgatcagct cgctcacgtt gacaccaccg tccccactcg ctcgccggac actatccgtt
     3601 cgatgttgcg ctcgtcgtcg atgttgaaga cgtagatcgg cagcgagttg tccatgcaga
     3661 gcgaaagcgc cgtcgaatcc atcacgccca ggcgtcgctc gatcgcctgg tggtgggtga
     3721 tctcgggcag gaagtgcgcg tcgggagtgg tgcgcggatc ggcgtcgaac acgccctcca
     3781 ccccgttctt cgccatcagc atcgcctcgg cgtggatctc gagcgctcgc agcgcggccg
     3841 cggtatccgt ggtgaagaac gggttgccag tgcccgcggc gaagatcacg atccggccct
     3901 tctcgaggtg gcggatcgcg cggcggcgga tgtagggctc ggcgacctcg gagattgtga
     3961 tcgccgactg cacgcgcgtg tgcgcgccga gcttctcgag cgcgtcctgc agcgcgagcg
     4021 cgttcagcac cgtggccagc atccccatgt agtcgcccgt ggcgcggtcc atgccggccg
     4081 cggcgccctg caggccgcgg tagatgttgc cgccacctac gaccaccgcc atctccaccc
     4141 cgcgctccac ggtgagctgg atctgacggg cgatcgcctg tatccggtcc gcgtcggcgc
     4201 cgtagggctg atcgcccatc agcgcctctc cggagagctt cagcagcaca cggcgaaagg
     4261 cgggcgccgc aaccgcgccg tcccgtcccc cttcgccggc catcgctgct ctactcctcg
     4321 cccaccacgt agcgcgtgaa gcgcctgatg acgatgttct cgccggtgtt cgcggcgatc
     4381 tcggcgcgca gctcctcgat cgtcttgccg tcgtgctcgt cgcggcggat gtgctcctgg
     4441 ttgagcagga cgttctcctc cagccacttg cgccaccggc cctcgacgat cttcgggcga
     4501 gcctgctccg gcttgtcctg agcctgctcc tcgtagatgc gctgctcggc ctggcgtatc
     4561 tcgtccggca cttcgtcctc cgacacgtac tgcggtgccg ctgccgcgat gtgcagggcc
     4621 acctcgcgca caaattcgcg gaagtcgtcg ttgcgcgcaa cgaagtcggt ctccgagttg
     4681 acctcgacga gcacaccgat cttgccgccg gcgtggatgt agctctcgac gagcccctcg
     4741 gacgccgtgc gaccgccgcg cttggcggcc tgcgcttgcc ccttcacgcg aaggatctcg
     4801 acggccttgt ccacgtcgcc gcccgcttcc tggagggccg ccttgcagtc catcatgccg
     4861 gcccccgtgc ggtcacggag ggccttcaca tcggatgcct tgatctctgc ggtactcact
     4921 gctggctcac ttcctcagtc tcatttggtg cctctgccgg tgccggcgcc tgctcctgcg
     4981 gagcggcggg cgcctcgccc tgctgctgcg cggcggcctc ggcctgctgc gctgcggccg
     5041 cggcctgctg ggcctgctcg gcctcgcggg cggcctgctc ctcggcctc
//
LOCUS       DASVXS010000274         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_609304, whole genome shotgun sequence.
ACCESSION   DASVXS010000274 DASVXS010000000
VERSION     DASVXS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609304"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF752_15455"
     CDS             <1..183
                     /locus_tag="VF752_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856991.1"
                     /translation="KSHTTLLAGAAGVATLLLVAVGISSAPLWAALLAALIVFGAGYW
                     WFRRAFRRHSGGLTFR"
     gene            complement(264..680)
                     /locus_tag="VF752_15460"
     CDS             complement(264..680)
                     /locus_tag="VF752_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856992.1"
                     /translation="MGEIATVDAAPQHLQALQRANRVRLARAELKRKVSANEITAAQV
                     VLDCPWQAESMSVSDLLMSQRRWGRARCRRLLLSLGIPENKQIGTLTGRQRTALAAML
                     LAKCPPKPAHLERPSYDGPRFESEGFAPREREAALA"
     gene            968..2383
                     /gene="pyk"
                     /locus_tag="VF752_15465"
     CDS             968..2383
                     /gene="pyk"
                     /locus_tag="VF752_15465"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX8856993.1"
                     /translation="MRATKIVGTIGPASLEREVLERMIVAGLDIARLNFAHGTPDDHA
                     EAVAEIRAAAEAVGREVCVLQDIPGPKLRIGPVEGGVCELGTGTNIVLTPEPVEGDAQ
                     RLPVAWPGFSEIVEPGDVAYLADGAIRLRVNAIHDGEVLARVEVGGPVASRQGLNLPN
                     VTMSLPAVSGEDLALIDAGVSMGVDVVALSFVRRREDLDPVRERLRDRGADTPIYAKI
                     EKPQAAANAEEIIDAADGIMVARGDLGIELPIEEVPLVQKKLLALAGHKAMPTITATQ
                     MLESMVTSSRPTRAEVTDVANAIFDGTDAVMLSQETAVGRYPVEAVAMMASIAETVEK
                     ELPYGRWLSERGLDAGEHESAAMAFGAVGAVYQLDLKALITPTISGRIARLVSSKRPQ
                     APVIALCPRLSVVRRCNLLWGVYAFLNEEPQDTTDLLDEAAEAALATGIVTPGQKVGI
                     TAGLPAGLSGGTNLFTVRTLQ"
     gene            2573..3844
                     /locus_tag="VF752_15470"
     CDS             2573..3844
                     /locus_tag="VF752_15470"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8856994.1"
                     /translation="MPAQAPLVLTHPMRVALLAGELVSNRLRARPHLRLILPTGHTPL
                     GMYEALRGHAADGSLWSGEATLFQLDEYVGLGPADERSYNAYLRRELAGVRFRTFHGL
                     DGSASDLDAECARHQALLDEAPIDLVVLGLGRDGHVAFDEPGSPVNAGVRRVRLHDVT
                     RADAAADFGGLDAVPEEALTVGLRTLLGARELLMLVSGSAKAEALRAMLEGEQGPQCP
                     ASYLRDHPRLTVVCDEDAARLLGPRPGSSSDRAIIVLGHREPGISADDRLSDESIARL
                     ERAMRECRREPPRTLLFTGYSRTRDGLSEAEQMKAAWDESEVPALLEDAGRNTSENAT
                     CSLPLIRAIGEIRRVTVVTSVWHIRALYFFAPYRSFGLKLSFAPELEGPWVRLLGHEL
                     HGLLGMRRQRRRAMAGMRLPAELALLPPEPE"
     gene            3841..>4047
                     /locus_tag="VF752_15475"
     CDS             3841..>4047
                     /locus_tag="VF752_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856995.1"
                     /translation="MSELIAADLALGGRVAPGWVEHDRGRVLGAAHGEPLRVPDLVLD
                     GVVSAGLCDLQVNGAAGHEVADGEE"
BASE COUNT          567 a         1378 c         1475 g          627 t
ORIGIN      
        1 aagtcgcaca ccacgctgct ggccggtgcc gccggcgtcg ccaccctgct gctggtcgcg
       61 gtgggcatct cttcggcgcc gctttgggcc gccctgctcg ccgcactgat cgtcttcggc
      121 gccggctact ggtggttccg ccgcgcgttc aggcggcact ctggcggact cacgtttcgc
      181 tagatgtcac cgcgcgccgc ccggcgaggg catgtgtcgg ggagatcgcc gggcgcacgc
      241 ggaactggcc ctctggtctg ggctcaggcc aatgcggcct cgcgttcgcg aggcgcgaag
      301 ccctcgctct cgaagcgggg gccgtcgtag gacgggcgct cgaggtgcgc gggcttcggg
      361 gggcacttcg ccagaagcat cgcggcgaga gcggtgcgct ggcgtcccgt gagggttcca
      421 atctgcttgt tctccggaat ccccagggag aggaggaggc ggcggcagcg agcgcggccc
      481 caacgccgct gactcatcag gaggtcgctc accgacatgc tctcggcctg ccaggggcag
      541 tcgagcacga cctgcgctgc ggtgatctcg ttggcactca ccttgcgctt caactcagcg
      601 cgtgccagac gcacgcggtt ggcgcgttga agggcctgaa ggtgctgagg cgcagcgtcg
      661 accgtggcaa tctcgcccat caagaaatct ccccgctatc actcgttttc ccacacgacc
      721 ccgtgcggtg cgtaagcccg ggtcggggtg ctcggtgctg gcgagagcac ccgacaagcc
      781 ctccgagtcc gtcgccccgc tggtcgtgtc cgaccagagg aaaactatgc cgtccctgac
      841 gcttgggtca acacggtcgt aacatgccca cccgctaaga gcgggctttt ctttgacagg
      901 ttcgtttgtc cagatttgga caagcgtttc ggtaccgaac tcgtgggata aggaagggca
      961 actgggcatg cgcgcgacca agatcgtcgg aacgattggg ccggcctcgc tggagcgcga
     1021 ggtgctcgag cgcatgatcg tggccgggct cgacatcgcc aggctcaatt tcgcccacgg
     1081 caccccggac gaccacgccg aggcggtggc cgagatccgg gcggccgccg aggcggtcgg
     1141 acgcgaggtg tgcgtgctcc aggacatccc cgggccgaag ctccggatcg gccccgtcga
     1201 gggcggcgtc tgcgagctcg gcaccggcac gaacatcgtg ctcacgcccg agcccgtgga
     1261 gggcgatgcc cagcgcctgc cggtggcgtg gccgggcttc agcgagatcg tcgagcccgg
     1321 cgatgtcgcg tacctcgccg atggtgccat ccggctgcgc gtgaacgcga ttcacgacgg
     1381 cgaggtgctg gcccgggtgg aagtcggcgg gcccgttgcc tcgcgccagg ggctgaacct
     1441 gccgaacgtg acgatgtcgc tcccggccgt gagcggcgag gacctcgcgc tgatcgacgc
     1501 gggcgtgtcg atgggcgtgg atgtggtcgc gctctcattc gtccggcgcc gcgaggacct
     1561 cgacccggtg cgggaacggc tgagggatcg cggcgccgat acgccgatct acgccaagat
     1621 cgagaagccg caggccgccg ccaacgcgga ggagatcatc gatgcggccg acggaatcat
     1681 ggttgcgcgc ggcgacctcg gcatcgagct gccgatcgag gaggtcccgc tggtccagaa
     1741 gaagctcctg gcgctcgccg gccacaaggc gatgccgacg atcacggcca cacagatgct
     1801 cgagtcgatg gtgacctcga gccgccccac tcgcgctgag gtcaccgacg tggcgaacgc
     1861 gatcttcgac gggacagacg ccgtgatgct gtcgcaggag accgccgtcg gccgctaccc
     1921 cgtcgaggcc gtggcgatga tggcgtcgat cgccgagacc gtcgagaagg agctgccgta
     1981 cggtcgctgg ctgtccgagc gcgggctcga cgccggcgag cacgagtcgg ccgcgatggc
     2041 gttcggggcg gttggtgccg tctatcagct ggacttgaag gcgctcatca cgccgacgat
     2101 ctcggggcgc atcgcccggc tcgtgtcgtc caagcggccg caggcgccgg tgatcgcgct
     2161 ctgtccgcgg ctgagcgtcg tgcggcgctg caacctgctg tggggcgtgt acgcgttcct
     2221 gaacgaggag ccgcaggaca ccaccgacct gctcgacgag gcggccgagg cggcgctggc
     2281 tacggggatc gtgacgccgg ggcagaaggt ggggatcacg gctgggctgc cggcggggct
     2341 ttccggcggc accaacctct tcacggtgcg gacgctgcag tagcgctcgg gcccgggcca
     2401 cgcggcggtc cggaccagca gcgccaacgg agcggcgcgg gtcggcccaa ggtgctcggg
     2461 ccgacctggg ccgttttgac attgatagtc atggtgaaaa cggcccaggt cggtggccgg
     2521 cggcgctcgg gcccggtagc gcggagcgtt attgcggtta cggtgatgcc cgatgcccgc
     2581 ccaggcgcca ctcgtgctca cccatccgat gcgggtcgcg ctcctcgcgg gcgagctggt
     2641 gtcgaaccgg ctgcgcgcgc gcccgcacct tcgcctgatc ctgcccaccg gccacacgcc
     2701 gctcggcatg tacgaggcgc tgcggggtca tgctgccgac ggcagtctgt ggagcggcga
     2761 ggcgaccctt tttcagctcg acgagtacgt cggcctcggg ccggcggacg agcggagcta
     2821 caacgcctat ctcaggcgag agctggcagg cgtgcggttc cgcacgttcc atggtctcga
     2881 cggctccgcc tctgacctcg acgcggagtg cgcccgtcac caggcgctgc tcgacgaggc
     2941 gcccatcgac ctggtcgtgc tcggcctggg tcgcgacggg cacgtggcgt tcgacgagcc
     3001 gggctctccg gtcaatgcgg gcgtgcggcg cgtacggctg cacgacgtca cgcgtgcgga
     3061 cgccgccgcc gacttcggcg gtctcgatgc ggtgcccgag gaggcgctca cggtggggct
     3121 gcgcacgctg ctcggcgcgc gggaactgct gatgctcgtg tccggatccg ccaaggcgga
     3181 ggcattgcgc gcgatgctcg agggcgagca gggcccgcaa tgcccggcgt cgtatctgcg
     3241 cgatcacccg cgcctcaccg tcgtctgcga cgaggacgcg gcgcgcctgc tcggcccgcg
     3301 gccgggctcg tcgagcgacc gcgccatcat cgtcctgggc caccgcgagc ccggaatcag
     3361 cgccgacgac cgcctgtccg acgagtcgat cgcgcgcctc gagcgcgcca tgcgcgagtg
     3421 ccggcgcgag ccacctcgca cgctgctctt caccggctat tcgcgcacgc gggacggcct
     3481 gtcggaggcc gagcagatga aggctgcctg ggacgagagc gaggtaccgg cgctgctcga
     3541 ggacgctggt cgcaacacct cggagaacgc cacctgctcc ctgcctctga tccgcgcgat
     3601 cggcgagata cgccgcgtga ctgtcgtgac ttccgtatgg cacatccgcg cgctctactt
     3661 cttcgcgccc taccgctcgt tcgggctcaa gctgtcgttc gctccggagc tggaagggcc
     3721 gtgggtgcgc ctcctcgggc acgagctgca cggactgctc gggatgcggc gacagcggcg
     3781 ccgcgccatg gccggcatgc gactgcccgc cgagctcgca ctactgccgc cggagcccga
     3841 atgagcgagc tgattgcggc agacctcgcc ctcggcggcc gcgtcgcgcc gggctgggtt
     3901 gagcacgacc gcggccgcgt gctgggcgcg gctcacggtg agccgctgcg cgtgcccgat
     3961 ctcgtgctcg atggagttgt ctccgccggc ctctgcgacc tgcaggtgaa cggcgccgcg
     4021 ggacacgagg tggcggacgg cgaagag
//
LOCUS       DASVXS010000275        10298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_761111, whole genome shotgun sequence.
ACCESSION   DASVXS010000275 DASVXS010000000
VERSION     DASVXS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761111"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VF752_15480"
     CDS             complement(<1..316)
                     /locus_tag="VF752_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8856996.1"
                     /translation="MEVNLESPLPASLPVGRATAIFCSGSSSHDVRRIEIVVDGERHP
                     VAAAGMPRPDLAHERSGFWGTVQVPAHDAPGAVELEGAVRLADGREAIVPLARVPVVE
                     PPP"
     gene            363..1334
                     /locus_tag="VF752_15485"
     CDS             363..1334
                     /locus_tag="VF752_15485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8856997.1"
                     /translation="MSAPTVVIPNWNGAHWLPGCLASLEAQTVRPAEIVVVDNGSTDG
                     SLELLRSRSPALRVIELGRNTGFAFAANRGIEAAGGDTVALVNTDVELAPDWLERTSR
                     VLAEHPEAASVACKMLDLRDPEVLYDAGDVLRRDGACVQRGRFCRDDGSYDQPGEVFA
                     PCAGAALYRRDSVLAAGGFDERFFAYLEDVDLGLRLRLAGHACRYEPVVARHAGEGSS
                     GALDPPVEAWVERNTLLLVARAFPFRWLPLVLYRQLGWAWHALRERRLGTHLRGAAAA
                     LPLLPAMLRERRALRRSAVLPVEHAIPSQPIRGPHAAGHPSARVSRR"
     gene            complement(1321..2499)
                     /locus_tag="VF752_15490"
     CDS             complement(1321..2499)
                     /locus_tag="VF752_15490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8856998.1"
                     /translation="MDRLAAQSRRIALVHDFLVDMRGAERVFLAMCEMWPHADVFSPI
                     YDEAGTEGRFAGREIRTSFLQKVKPTARTFRALLPLYPAAIESFDFSGYDVVVSSSSA
                     WAHAVICGEGTVHVSYCHNPFRYAWSERDATLAARRDPFSRAALRGIFRRWRQWDWIA
                     AQRVDRYIANSHMTQARIRAYFGRESTVVYPAVETGRFAPGKVSDYFMVLSELMPHKR
                     IDVAIRAFNKLRLPLVIVGDGPERRHLQHIAGPTISFTGRVSDRRVAQLLSGARALIV
                     PAVEEFGIAAVEAQAAGRPVISARGGQQESVLDGITGAFFEGEEDELIKVVTAFDESA
                     VDPTECVANATRFDTTVFRERLPHEVERAIEDSQRAPREELRVMSRRRRRARTHVSAD
                     "
     gene            2587..2943
                     /locus_tag="VF752_15495"
     CDS             2587..2943
                     /locus_tag="VF752_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX8856999.1"
                     /translation="MPRPRRLAAAAAALALALPASALADDSAGDQQYQDPFGNNPPGQ
                     TQPGSQSQPGSQTQGSGSSGGGTSQPAPPQAAQPVATTAAPSQELPRTGVNAGLLVAL
                     GAAMLAAGVAIRRRLA"
     gene            2999..5014
                     /locus_tag="VF752_15500"
     CDS             2999..5014
                     /locus_tag="VF752_15500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8857000.1"
                     /translation="MSYSGAFGGAERVLLDVAANYEGAVTIACAAGELAARARDRKLP
                     VVELPARPLELRGPAPTRLAAARALGAHAREIRRLARDLAPEVLVAWGARSALAAAPG
                     LAGKPGAPALVVQLNDLVPGPAIGRALRAACRRATAVIVPSRAVARDLGVPVTVIPPA
                     VDLDHFAAVPEPPNKAAVLVLGAITDVKQPGLALEAAAIASRDIPELALTLAGAPLGE
                     DGGRLLARLERRGGEPDLAGRVHFAGQVADPSDALAAADCLFHAGSREAFGLNLVEAL
                     ASGRPVIAPASAGPLEIVDDGCGRLYRPDDPEAAARALVELLADGELRERLGRAGRER
                     ARECFDIRKTRRRYAELLGSVHPADRRAPEGSGIALVTVTHNSADDLPRLLDSAARHL
                     PGARVVVVDSGSSDGSAGLARERGATVIELGENAGFGRGSNAGLHEVSEPVTVLLNPD
                     VELLDSSLARAAEEVRNGPERILGPLVLLPSGERQDSAHAPPGSGAELAIALGPPALL
                     PSPLRTRVQPWRATSPRAAGWLVGCCLIARTETLRRLGPFDDRLFLYAEDMELGLRAR
                     EGGVEQWFWPHARVLHRAAHSSARAFGGEPFELLARQRRAIVAMRLGPQSLRRDDIAQ
                     AVTFASRISLKTLLRRDAGREREQLRALRAARREPPDPVVSAEELER"
     gene            5023..6039
                     /locus_tag="VF752_15505"
     CDS             5023..6039
                     /locus_tag="VF752_15505"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8857001.1"
                     /translation="MTIDARAAARREIGGVERWARELAARLPRTTSGRYAVAQPRPAL
                     AHRAGHAWEQAVLPLRARGSQFVLCPANLAPLASGRNVVVVHDVAPLRHPEWYGRAYV
                     AWQRALLPAIARRAVRLVAVSEFARRELVEVLGANPDRTVVIPGGVDERFKPDAPRPL
                     GIHRPYVLTVGTRIARKNLGALRATAEMLERRGIELVSAGSGRGYMRAEDAPPVRAFG
                     YVPDEDMPGLYAHAEAFVMPSLYEGFGLPCIEAMACGTPVVAADRAALPETCGDAALL
                     ADPEDGAAFSAVVERVLDDEALRTRLREAGLARARSLTWDRTAGEVDALIGRLLRSAI
                     SPSP"
     gene            complement(6020..7552)
                     /locus_tag="VF752_15510"
     CDS             complement(6020..7552)
                     /locus_tag="VF752_15510"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8857002.1"
                     /translation="MRRTLPLLLAAAAVAAVPTSASARGFSYGVAAGEVTAKSALLWA
                     RADKAGSYTLQVSTDRRFRRFVDERVVKATKNHDNTLQRTIKGLKPDTTYYYRFVNGS
                     KRSSVGKFRTAPDASKAKTIKFAFSGDADAEPGVGSTKPFYNNFQAYRSMARAGNLFN
                     VNLGDVIYSDSEVPGKGALATTVKQKWEKYRQNLALRNLQLFRGAAGSYSHWDDHEFA
                     NDFTKAENGSAVYKAGVRAFRDYMPVTYSTNRGIYRTFRWGKNVELFFLDERSFRSAK
                     ASANHACDNPQTGGPDLAPTGPPATRALFAVAVPSLAAPVSQQCLDTINDPKRTMLGS
                     KQLAAFEKAVKASSAKWKLIMNEVPIQQYYALPYDRWEGYAAERTKLLQFLSANVKNV
                     AFLSTDVHANMVNDARFQTLEAGGPKDSGIMDFASGPVATMTYVKEIDRTTGREGNGK
                     LITDAFFKPPPPNGVGMKCAQTNVFSYAQVTVNSKQLKVAYRDQDGKALPDCGQFTVK
                     AK"
     gene            complement(7614..8759)
                     /locus_tag="VF752_15515"
     CDS             complement(7614..8759)
                     /locus_tag="VF752_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857003.1"
                     /translation="MRVKKVCLLAVLAALACLGSTASAEAGRTVPQGFFGTVLDGGLL
                     QAPAGVQDAQLGRMSLNGVESIRTSFSWAAVQPNEGAPPDFASTDRLVASAAVHGLSL
                     LPVVVYAPAWARADASAFNSPPRDPEQYAAFLTVLIGRYGPSGSFWQEHPELPKRPLR
                     EWQIWNEPHLRLYWNSPRWEQGYGALLRASRRAIRAADPRAKVVLAGLTGFSWDALAA
                     LYRSGHIRHQFDVAALQTYTGPPRLLLKATRLFRQVLARHGDARTPIWITEFGWPAAR
                     GRTKVPSYQRNIATTDRGMAQRLSGAYRLFAAVRSRPDVRVERLYWYTWSAPYTRSND
                     PGTGIFRFAGLNRYDGSSFRATPALRAYRTAARSLEGCVKTQTGSCR"
     gene            8850..10187
                     /locus_tag="VF752_15520"
     CDS             8850..10187
                     /locus_tag="VF752_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8857004.1"
                     /translation="MRNVCVIGAGSSGIAACQVLASRGIPFDCFEKGSGVGGNWRYEN
                     DNGMSSAYHSLHINTSRHLMEYASYPMPESYPDYPSHWEIARYFDDFVDHFGLRERIT
                     FNTAVTKIERSDDDGWHVTLENGETRSYSAVLVANGHHWNPRWPWFPGEFHGDVMHAH
                     HYKTPEGFEGRDVIVLGFGNSAVDIACELGRVASNTYLATRRGAHVLPKYLRGKPTDE
                     AAPALAHFIPMNIAGRLMERQLRRLQGAMTDYGLPEPDHHIGEAHPTMSSELLPAIGH
                     GRVTPKPNISRLEGDDVRFADGTVVHADRIVYCTGYKITFPFLEKQVLNPDENQVHLY
                     RHVVHPEQPGLYFLGLIQPLGAIMPIAERQAEWIADVVTGEGALPSREEMLHEIERHR
                     ERMAKRYVRSPRHTIQVDFHPYMRELARERKRSRRRARRPQRALAPELAPARA"
BASE COUNT         1430 a         3728 c         3615 g         1525 t
ORIGIN      
        1 ccggcggcgg ctcgacgacc ggcacccggg cgagcggcac gatcgcctcg cggccgtccg
       61 cgaggcgtac ggcgccctcg agctccaccg cgccgggcgc gtcatgtgcc ggcacctgca
      121 cggtccccca gaaaccgctc cgctcgtggg cgaggtcggg tcgcggcatc ccggccgctg
      181 cgaccggatg gcgctcgccg tctaccacga tctcgattcg cctcacgtca tgggagctgc
      241 tccccgagca gaagatcgcc gtcgccctcc ccaccggcag cgacgccggc agcgggctct
      301 cgaggttcac ctccatgacg cgctgagagt agaccggtcc gggcggctag gctgggcccg
      361 cggtgagcgc gccgactgtc gtgatcccca actggaacgg cgcccactgg ctgccgggct
      421 gtctcgcttc gctcgaggcg caaacggtgc gaccggcgga gatcgtggtg gtcgacaacg
      481 gctcgaccga tggatcgctc gagctgctgc ggtcgcgcag ccccgccctg cgtgtgatcg
      541 agctcggccg caacaccggc ttcgcgttcg cggcgaaccg cggcatcgag gccgcgggcg
      601 gcgacaccgt cgcgctcgtg aacacggacg tcgagctcgc gcccgactgg ctcgagcgca
      661 cgtcgcgcgt gctggcggag catcccgagg cggcgtcggt ggcgtgcaag atgctcgacc
      721 tgcgcgaccc cgaagtgctg tacgacgccg gcgacgtgct gcgccgcgac ggcgcctgtg
      781 tgcagcgcgg gcgcttctgc cgggacgacg gcagctacga ccagcccggc gaggtgttcg
      841 cgccgtgcgc cggcgctgcg ctctaccggc gcgactcggt gctcgcagcc ggcggcttcg
      901 acgagcgctt cttcgcctac ctcgaggacg tcgacctcgg gctgcgtctg cgcctcgccg
      961 ggcacgcttg ccgctacgag ccggtcgtcg cgcggcacgc gggcgagggc agctccggcg
     1021 ctctcgaccc gccggtcgag gcctgggtgg agcgcaacac gctgctcttg gtggcgcgtg
     1081 cgttcccgtt ccgctggctg ccgctcgtgc tctaccggca gctcggctgg gcctggcacg
     1141 cactccgcga gcggcggctg ggtacacacc ttcgcggtgc ggcggccgcg ctcccgctgc
     1201 tgccggcgat gctgcgcgag cggcgcgcgc tgcggcgctc ggcggtgctg ccggtcgagc
     1261 acgcgatccc ctcacagccg atccgcggcc cccacgccgc gggccacccg agcgcgcgcg
     1321 tcagtcggcg ctgacgtgcg tgcgcgcgcg gcgccggcgg cgcgacatca cacgcagctc
     1381 ctcgcgcgga gcgcgctgcg aatcctcgat cgcccgctcg acctcgtgcg gcagccgctc
     1441 gcggaagacc gtggtgtcga agcgtgtcgc attcgcgacg cattcggtcg gatcgaccgc
     1501 ggactcgtcg aacgccgtca cgaccttgat cagctcgtcc tcctccccct cgaagaacgc
     1561 tccggtgatg ccgtcgagca ccgattcctg ctgaccgccg cgcgccgaga tcaccggtcg
     1621 cccggcggcc tgcgcttcga ccgccgcgat gccgaactcc tccacggccg gcacgatcag
     1681 cgcccgggcg ccgctcagca gctgggccac ccgccgatcg ctcacgcggc ccgtgaacga
     1741 gatggtcggg ccggcgatgt gctggaggtg gcggcgctcg ggcccgtcgc ccactatcac
     1801 gagcggcagc ctcagcttgt tgaacgcgcg tatcgcgacg tcgatccgct tgtgcggcat
     1861 cagctcggac agcaccatga agtagtcgga caccttgccc ggcgcgaagc gcccggtctc
     1921 caccgccggg tagacgactg tcgactcgcg cccgaagtag gcgcggatgc gcgcctgcgt
     1981 catgtgcgag ttcgcgatgt agcggtccac gcgctgcgcc gcgatccagt cccattggcg
     2041 ccaccgccgg aagatcccgc ggagcgccgc gcggctgaag gggtcacgcc gggccgcgag
     2101 cgtcgcgtcg cgctcgctcc acgcatatcg gaacgggttg tggcagtagg agacgtgcac
     2161 cgttccctcc ccgcagatga ccgcatgcgc ccacgcgctc gagctcgaga cgacgacgtc
     2221 gtagcccgag aagtcgaacg actcgatcgc cgcgggatag agcggcagca gcgcccggaa
     2281 cgtgcgcgcc gtcggcttca ccttctgcag gaacgaggtg cggatctcgc gccccgcgaa
     2341 ccggccctcg gtgccagcct cgtcgtagat ggggctgaac acgtccgcgt gcggccacat
     2401 ctcgcacatc gcgaggaaca cacgctccgc gccgcgcatg tccacgagga agtcgtgtac
     2461 gagcgcgatc cgtcgcgact gcgcggccag gcggtccatg ccagccgagt gtaatcggca
     2521 tgtgaagttc tcgcctgaac cgttcgggcg cggaagcgcc aggaacgtct gctaggaatt
     2581 caggcgatgc cccggccacg tcgactcgcc gcagcggccg ccgcgctcgc gcttgcgctg
     2641 cccgcgtccg cgctcgccga cgacagcgcc ggcgaccagc agtaccagga ccccttcggc
     2701 aacaacccgc cgggccagac ccagccgggg tcccagagcc agccgggatc gcagactcag
     2761 ggaagcggct cgtccggtgg cggcacgagc cagccggcgc cgccgcaggc ggcgcaaccg
     2821 gtcgccacga ctgcggctcc ctcacaggag ctgccacgca cgggcgtgaa cgcgggcttg
     2881 ctcgtggcgc tcggggccgc catgctcgcc gcgggcgtcg cgatccgccg gcgcctggcc
     2941 tgacgatcca acgcaaagga cacgggaccc cgctctgagc ctcccgcgga cgttgttcgt
     3001 gagctactcc ggcgccttcg gcggagccga gcgcgtgctt ctcgatgtgg ccgcgaacta
     3061 cgagggcgcg gtcacgatcg cgtgcgccgc cggcgagctc gccgcgcgcg cacgcgaccg
     3121 aaaattgccg gtcgtggagc tcccggcgcg gccgctcgag cttcggggac ccgccccgac
     3181 acggctagcc gcggcgcgcg cgctcggcgc gcacgcccgc gagatccggc ggctggcgcg
     3241 cgatctcgcc cccgaggtgc tcgtggcctg gggcgcgcgc tccgcgctcg ccgcggcgcc
     3301 gggcctggcg gggaaaccgg gcgcaccggc gctcgtcgtt cagctcaacg acctcgtgcc
     3361 cgggcccgcg atcgggcgcg cgttgcgcgc cgcgtgccgg cgcgcgacgg cggtgatcgt
     3421 cccgtcgcgg gcggtggcac gcgacctcgg tgtgccggtc accgtaatcc cccccgcggt
     3481 cgacctcgac cacttcgcgg cggtgcccga gccgccgaac aaggcggcgg tactcgtgct
     3541 cggggcgatc accgacgtga agcagccggg gcttgcgctc gaagccgcgg caatcgcgtc
     3601 gcgtgacatc cctgagctcg cgctcacgct tgccggcgct ccactgggcg aagacggtgg
     3661 ccgcctgctc gcgcggctcg agcgtcgggg aggcgagcca gacctcgccg gccgcgtgca
     3721 cttcgccggc caggtggccg accccagcga cgcgcttgcc gcagcggact gcctcttcca
     3781 cgcgggctcc cgcgaggcgt tcggtctcaa cctggtggag gcgctggcga gcggacgccc
     3841 ggtgatcgcg ccggcgtcgg cgggaccgct cgagatcgtc gacgacggct gcggccgcct
     3901 ctaccggccc gacgacccag aggccgccgc gcgagccctc gtggagctgc tcgccgacgg
     3961 cgagctgcgc gagcggctgg gcagggcagg ccgcgagcgg gcgcgcgagt gcttcgacat
     4021 ccgcaagacg cgccgccggt acgccgagct tctcggctcg gtccacccgg cagaccgacg
     4081 ggctccggag ggcagcggca tcgcgctcgt caccgtcacg cacaactcgg cagacgacct
     4141 gcccaggctg ctggactccg ccgcgcgcca cctgcccggc gcccgcgtcg tcgtggtcga
     4201 ctcgggctcg agcgacggca gcgccgggct cgctcgcgag cgcggcgcga ccgtgatcga
     4261 gcttggcgag aacgccggct tcggccgcgg ctcgaacgcc gggctgcacg aggtgagcga
     4321 gccggtcacg gtgctcctga acccggacgt cgagctgctc gattcctcgc ttgcgcgcgc
     4381 ggcggaggag gtccgcaacg ggccggaacg gatcctcgga ccactcgtgc tgctcccgag
     4441 cggcgagcgc caggacagcg cgcatgcgcc gcccggttcc ggcgcggagc tcgcgatcgc
     4501 gctgggaccg ccggcccttc tgccctcgcc gctccgcacg cgagtgcagc cgtggcgggc
     4561 cacgagcccg cgagcggcgg gctggctcgt gggctgctgc ctgatcgcgc ggaccgagac
     4621 gcttcggcgc ctcggcccgt tcgacgacag actgttcctc tacgccgagg acatggagct
     4681 cgggctgcgc gcgcgagagg gcggagtcga gcagtggttc tggccgcatg cacgcgtgct
     4741 ccaccgcgcg gcccactcgt ccgcacgcgc attcggcggc gagccgttcg agctgctcgc
     4801 gcgtcagcgg cgcgcgatcg tggcgatgcg gcttgggcct cagtcgctgc ggcgcgacga
     4861 catcgcgcag gcggtcacct tcgcgagccg aatctcgctc aagacgctgc ttcgccgcga
     4921 cgccgggcgc gagcgcgagc agctgcgagc gttgcgcgca gcgcgtcgtg aaccgcccga
     4981 cccggtggtg agcgctgagg agctcgagcg ctgagcgcgc ccgtgacaat cgacgcgcgc
     5041 gctgccgcga ggcgcgagat cggcggcgtg gagcgctggg cgcgcgagct cgccgcgcgc
     5101 ctgccgcgga ccacgtccgg ccgctacgcc gtcgcacagc cgcggcccgc gctcgcgcac
     5161 cgcgccggcc acgcgtggga gcaggcggta ctgccgctaa gagcacgcgg ctcgcagttc
     5221 gtcctctgcc ccgccaacct ggccccgctc gcgagcggtc gcaacgtggt ggtggtacac
     5281 gacgtcgcgc cgctgcgcca ccccgagtgg tacggccgcg cgtacgtcgc gtggcagcgg
     5341 gcactcctgc ccgcaatcgc gcgccgcgcg gtgcggctgg tggcggtgtc cgagttcgcc
     5401 cggcgggagc tcgtggaggt gctcggtgcg aacccagaca ggacggtcgt gatcccgggc
     5461 ggagtggacg aacgcttcaa gccggacgcg ccgcggccgc tcggcatcca tcgcccgtac
     5521 gtgctcaccg tgggcacgcg gatcgcgcgc aagaacctcg gcgcgctccg cgccaccgcc
     5581 gagatgctcg agcgccgggg catcgagctc gtgtccgccg gctcgggccg cggctacatg
     5641 cgcgccgagg acgcgccgcc ggtgcgcgcg ttcggctacg tgcccgacga ggacatgcca
     5701 ggcctgtacg cgcacgccga ggccttcgtg atgccctcgc tctacgaggg cttcgggctc
     5761 ccgtgcatcg aggcgatggc gtgcggcacg ccggtcgtgg cggccgatcg cgcggcgctg
     5821 ccggagacct gcggcgacgc cgcgctgctc gccgaccccg aggacggggc cgctttcagc
     5881 gccgtcgtcg agcgcgtgct cgacgacgaa gcgttgcgca cgcggcttcg cgaagcgggc
     5941 ctcgcccggg cgcggtcact cacctgggac cgcaccgcgg gcgaggtcga cgccctgatc
     6001 gggcgccttc tccgcagcgc tatttcgcct tcaccgtgaa ctgcccgcag tccggcagcg
     6061 ccttgccgtc ctggtcgcgg taggccacct tcagctgctt cgagttgacg gtcacctgcg
     6121 cgtagctgaa cacgttggtc tgagcgcact tcatccccac gccgttcggc ggcggcggct
     6181 tgaagaacgc gtccgtgatc aacttgccgt taccttcacg gcccgtcgtg cggtcgatct
     6241 ccttgacgta cgtcatggtc gccaccggac cgctcgcgaa gtccatgatc ccgctgtcct
     6301 tcgggccgcc ggcctcgagc gtctggaagc gcgcgtcgtt gaccatgttt gcgtggacgt
     6361 cggtcgagag gaacgcgacg ttcttcacat tcgcggacag gaactggagc agcttcgtcc
     6421 gctccgcggc gtagccctcc cagcggtcgt acggcagcgc gtagtactgc tggatcggca
     6481 cctcgttcat gatcagcttc catttcgcgc tcgatgcctt caccgctttc tcgaaagcgg
     6541 cgagctgctt cgagccgagc atcgtgcgct tcgggtcgtt gatcgtgtcg aggcactgct
     6601 gcgacacggg cgcggccagc gacggcacgg ccaccgcaaa cagcgctcgg gtggccggcg
     6661 ggccggtggg cgcgagatcc ggcccgccgg tctgcgggtt gtcacaggcg tggttcgcgg
     6721 acgccttcgc gctgcggaac gagcgctcgt ccaggaagaa gagctcgacg ttcttgcccc
     6781 agcggaacgt ccggtagatg ccccggttcg tcgaatacgt gacgggcatg tagtcgcgga
     6841 acgcccgcac gccggccttg tagaccgcgg acccgttctc cgccttggtg aagtcgttgg
     6901 cgaactcgtg gtcgtcccag tggctgtagc tgcccgcagc tccgcggaac agctgcaggt
     6961 tcctcagcgc caggttctgc ctgtacttct cccacttctg cttcaccgtg gtcgcgaggg
     7021 cgcccttccc cggcacctcg gagtccgagt agatgacgtc gcccaggttg acgttgaaca
     7081 ggttcccggc gcgcgccatc gaccggtacg cctggaagtt gttgtagaag ggcttcgtcg
     7141 atcccacgcc gggctcggcg tcggcgtcgc ccgagaaggc gaacttgatc gtcttcgcct
     7201 tgctcgcgtc tggcgcggtg cggaacttgc cgactgagct gcgcttcgag ccattcacga
     7261 agcggtagta gtacgtcgtg tcgggcttca gtcccttgat ggtccgctgg agcgtgttgt
     7321 cgtggttctt cgtcgccttc acgacgcgct cgtcgacgaa ccggcggaag cgcctgtcgg
     7381 tggacacctg cagcgtgtag gagccggcct tgtccgcgcg cgcccagagg agcgccgact
     7441 tcgccgtgac ctccccggcg gccacgccat agctgaatcc cctcgccgac gcgctcgtgg
     7501 gcacagccgc cacagcggcg gccgcgagca gcagcggcaa cgtccttcgc atcgacacgt
     7561 cgtcccccct cctgggccgt tgacccgtta gcggttgagc accctacagg cagtcaccgg
     7621 cagcttccgg tctgcgtctt cacacatccc tcaaggctgc gcgccgcggt gcggtaggcg
     7681 cgcagcgccg gtgtcgccct gaagctgctt ccgtcgtagc ggttcaggcc tgcgaagcgg
     7741 aagatgcccg tccccggatc gttcgagcgg gtgtatgggg ccgaccaggt gtaccagtag
     7801 agccgctcca cgcgcacgtc cggcctgctg cgcaccgctg cgaacaggcg gtacgcgccg
     7861 ctcagccgtt gcgccatgcc gcgatcggtg gtggcgatgt tgcgctgata gctcggcacc
     7921 ttggtgcggc cgcgcgcggc cggccagccg aactcggtga tccagatcgg cgtgcgcgca
     7981 tcgccgtgcc tggcgagcac ctgccggaac agccgcgtcg ccttgagcag gagccttggc
     8041 ggcccggtgt acgtctgcag cgcggccacg tcgaattgat gccggatgtg gccactgcgg
     8101 tagagagccg cgagggcgtc ccaggagaat ccggtaagtc ccgcgagcac caccttcgcg
     8161 cgcggatcgg ctgccctgat cgcccttcgc gaggcacgga ggagcgcgcc gtagccctgc
     8221 tcccagcggg ggctgttcca gtagaggcgc agatgaggct cgttccatat ctgccactcg
     8281 cgcagcggcc gcttcgggag ctcgggatgc tcctgccaga agctcccgct cgggccgtag
     8341 cgtccgatga gcaccgtgag gaatgcggcg tactgttccg gatcgcgagg cggggagttg
     8401 aaggcactcg cgtccgcccg agcccacgcg ggcgcgtaga ccacgaccgg caggagcgag
     8461 aggccgtgga cggctgcgct cgccaccagg cggtccgtgg acgcgaagtc cggcggggct
     8521 ccctcgttcg gctgcaccgc cgcccacgag aacgaagtgc ggatcgattc gactccattc
     8581 agggacatcc ggccgagctg ggcgtcctga acgccggccg gcgcctgcag cagccctccg
     8641 tccagcacgg tgccgaagaa cccttgcggg acggtgcggc cggcctcggc ggaggccgtc
     8701 gacccgaggc acgcgagagc ggccagcacg gcgaggaggc agaccttctt cacgcgcatt
     8761 cggaacacgc gagcatacgg gtcgattcgg ctttccagac gacgatctcg aaagttgacg
     8821 cgcgcgtcat cggtcgtcag aatcgcctgg tgcgcaacgt ctgcgtgata ggcgccggct
     8881 cttctggcat agccgcctgc caggtgctgg catcgcgcgg cattccgttc gactgcttcg
     8941 agaaaggctc cggcgtaggc ggcaactggc gctacgagaa cgacaacggc atgtcgtcgg
     9001 cgtaccactc gctgcacatc aacacgtcgc gccatctcat ggagtacgcg agctacccga
     9061 tgccggagtc gtacccggac tatccgagcc actgggagat cgcacgctac ttcgacgact
     9121 tcgtcgacca cttcgggctg cgcgagcgga tcaccttcaa cacggcggtg acgaagatcg
     9181 agcgctccga cgacgacggc tggcacgtca cgctcgagaa cggcgaaacg cgcagctaca
     9241 gcgccgtgct cgtcgccaac ggccaccact ggaacccgcg ctggccctgg ttcccggggg
     9301 agttccacgg cgacgtcatg cacgcgcacc actacaagac gccggaggga ttcgaaggca
     9361 gggacgtgat cgtgctcggc ttcggcaact ccgcagtcga catcgcgtgc gagctcgggc
     9421 gcgtcgcgtc caacacgtat ctcgcgactc gccgcggcgc gcacgtgttg ccgaagtacc
     9481 ttcgcggcaa gccgaccgac gaggcggccc ctgccttggc gcacttcatc ccgatgaaca
     9541 tcgccggccg gctcatggag cgccagctga ggcgcctgca gggggcaatg accgactacg
     9601 gcctgcccga gccagatcac cacatcggcg aggcgcatcc gacgatgtcg tccgagctgc
     9661 tgccggcgat cgggcacggc cgcgtgacac ccaagcccaa catctcccgc ctcgagggcg
     9721 acgacgtgcg gttcgctgac ggcaccgtcg tgcacgccga ccgcatcgtg tactgcaccg
     9781 gctacaagat cacgttcccg ttcctcgaga agcaggtgct gaacccggac gagaaccagg
     9841 tgcacctcta tcggcacgtg gtgcatccgg agcaaccggg tctctacttc ctgggtctga
     9901 tccagccgct tggcgcgatc atgccgatcg cggagcgcca ggcggagtgg atcgccgatg
     9961 tggtcaccgg cgagggcgcg ctcccctcgc gcgaggagat gctgcacgag atcgagcggc
    10021 atcgcgagcg tatggcgaag cgctacgtgc gctcgccgcg tcacacgatc caggtcgatt
    10081 tccaccccta catgcgtgag ctcgcgcgcg agcggaagcg ttcgcggcgc cgcgcgcggc
    10141 gtccgcagcg agcgcttgcc ccggagcttg cgccggcgcg cgcctgacgc ctcggttaca
    10201 acgcctcgca ctagcggtta cacatgttgt cccccatagg gggtatcacc gtgttaggat
    10261 cggcgcggac tcgagggcga tttgaacacg agggcagc
//
LOCUS       DASVXS010000276        16696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1046152, whole genome shotgun sequence.
ACCESSION   DASVXS010000276 DASVXS010000000
VERSION     DASVXS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16696
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046152"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VF752_15525"
     CDS             complement(<1..537)
                     /locus_tag="VF752_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8857005.1"
                     /translation="MTLAARLRAPGWYRAALSIPIGVGLAFAIVVPIRALYGWHPIVD
                     GDAITTVALISTPLAFLIGIGCFDYWFRWASGAPTVPEDHSGHGAYTWRDYFRVNTDH
                     KVIGIQYIVTTFFFFIVGGLMAMLMRAELAAPGRQFVDSNTFNGLFSVHASLMIFLFI
                     IPVFAGIANYVLPLMIGAP"
     gene            complement(534..1601)
                     /gene="coxB"
                     /locus_tag="VF752_15530"
     CDS             complement(534..1601)
                     /gene="coxB"
                     /locus_tag="VF752_15530"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8857006.1"
                     /translation="MLLIGAVASVIGVAIALSIDWFPPLAADKVGEIDTLYDVLLIVS
                     VPIFVLVMTVAIYSVVKFRAKPGDLSDGAPIHGNTRLEIIWVSIPFLLVTALAAYGWV
                     VLHNVEASPAAMTVKVRGQQFTWHFTYEGPNGKPVTSDVLYLPNKERVKFDITTNDVI
                     HSFWVPQFRLKQDAVPGMTTHIKITPNKVGTYDVVCAELCGLGHSTMRNTVRVVEKPA
                     FTAWLARQARGPQQQAQAPAGGAAPQGGGGGNANAAGKQVFASNGCGGCHTLSDAGTS
                     GAVGPKLDDLAAAAAKFGKQNNETAAQYVKQSIVDPRAFVVPGFQPVMPTDFKDRISG
                     AQLDALVKYLLSVSGGKAGSG"
     gene            complement(1716..2081)
                     /locus_tag="VF752_15535"
     CDS             complement(1716..2081)
                     /locus_tag="VF752_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8857007.1"
                     /translation="MAKRQATVDRRRALFEEAVAIIEEDYSQELDLDQVAQRIATSRR
                     QLQRAFAEAGNTTFRAYLADVRMRRAMELLRTGRMPVRQVAMSVGYRQPAQFAKTFRR
                     HHGAPPSSFRPGEAAVAAV"
     gene            2321..2713
                     /locus_tag="VF752_15540"
     CDS             2321..2713
                     /locus_tag="VF752_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8857008.1"
                     /translation="MQVSDGMSSVILTVGPDHTIRDAARQMAERKVGAAIVIDPDQPG
                     PGIVTERDILESIAAGQSPDQERVANHLSANLTFAASDWSLERAAEAMVKGGFRHLVV
                     MDGGETVGILSMRDIVRCWTMDGATCDV"
     gene            complement(2880..4259)
                     /locus_tag="VF752_15545"
     CDS             complement(2880..4259)
                     /locus_tag="VF752_15545"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEX8857009.1"
                     /translation="MALTATTAQARDHCLAPSRTIDAPLANASKYGRMFPELPPVSEE
                     EPLVLALGRSGGFCDGSAMADDPGCDDARGAAGWPLFGQFIAHDITADRSSLQEHADP
                     DSVRNFRTPRANLECLYGAGQVGSPYLYDRDDAPKLLIGENDEGEPADVPRNRQGTAL
                     IGDPRNDVHLFVSQLHLAFLKVHNGLVDRLREDGVAEDELFDEAARATHWHYQWVIVH
                     DFLPRLVGADLMRSLLDSGPRFYRPQGDAFIPFEFADGAYRYGHGQIRHSYRLNDFSG
                     DLPLFPDLMGFRPVPAAREVDWSHLFEMPGAEPPQPAKKIDGRLARSLIELPLEITGD
                     VAVEEYHSLAVRDLQRGHALSLPSGESVARAMGEQPLTREQVGLADSGWSGETPLWLY
                     VLKEAEVRQDGERLGPVGGQIVAEVLLGIIDADPDSQRVVDPEWRPTLPSVNGNFELA
                     DLLRFASYR"
     gene            complement(4320..4559)
                     /locus_tag="VF752_15550"
     CDS             complement(4320..4559)
                     /locus_tag="VF752_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857010.1"
                     /translation="MAEQQEVSAHENVPPPGEEIHLPGPSYLPVLMAFAITLMLVGVV
                     YTWVLTGIGLVIFLVVLVRWIRSTREDISELPLEH"
     gene            4774..5772
                     /locus_tag="VF752_15555"
     CDS             4774..5772
                     /locus_tag="VF752_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8857011.1"
                     /translation="MSPVTVGVDVGGTKVAAAAVEGTKALDRLERPTDLNSSGAVVEQ
                     IEAVAREALSHAPEAEAVGVGVPSQIDFETGEVIASVNIPLRGVRLREELERKLNLPV
                     FVDNDGNVAAMAELHLFDEPDIRHLIMLTLGTGVGGGVVIDRHIFRGATGRGAELGHI
                     VVQADGPECPGDCPNHGCLEAYCSGLALERDATALGKEKPDSGLGRVVRERDGRCRGR
                     DAVELAREGDLEAVQLLQRLGTYLGVGMTSLMNAFEPEMIVIGGGLGQAADLFLNRAI
                     EETKSRVLPALEPRVRVERALGGPDAGVIGAGLLAAHEHKRAGGHTPEETIKEGAL"
     gene            5769..7820
                     /gene="tkt"
                     /locus_tag="VF752_15560"
     CDS             5769..7820
                     /gene="tkt"
                     /locus_tag="VF752_15560"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX8857012.1"
                     /translation="MTTSTTDTRTLDELSVNTIRTLAMDAVQKANSGHPGTPMALAPL
                     AYVLYTRVMKHDPADPEWFDRDRFVLSAGHASMLLYASLYLSGYGLEVEDLENFRQVG
                     SPTAGHPEYWHAKGIETTTGPLGQGISTSVGMALAERMLNARFGDEVIDHHTWVIASD
                     GDLEEGIASEACSLAGHLGLGRLIVFYDDNHISIEGDTELAFTEDVGKRFEAYGWHVI
                     NLGEQIDLESLERAAHQAKAVEDRPSLVIVRTHIAPGSPNKQGSAKAHGEPLGVEEVR
                     LTKEVYGWPSDKTFYVPDEVLEHFRSVTAKRGREAHEQWNSRFEEYRRQQPERSDELE
                     LFIERRLPDGWDSDVPRFDPNDEKVGSKLATRKASNAVIQWAAARVPQLVGGSADLAP
                     STLTLIDGGGDVEKGHYGGRNMHFGVREHAMGAIVNGLVLHGFRAFGATFLIFSDYMR
                     GSIRLSALMQIPSIFVFTHDSIGLGEDGPTHQPIEQLPGLRAVPNLYVVRPAGANETA
                     RAWEFALRQTNRPCAFALTRQGVPVWDPDGVPADCVERGAYVLRDASDGREPEVILIG
                     TGSEVHLCVEAQTLLEADGVATRVVSMPCVERFLEQDESYRDQVLPGSVRARVAVEAA
                     HPQTWHQFVGEAGAIVGMETFGASGPDKAVFQHFGFTPERVADEARAVIGRLSSAPAG
                     G"
     gene            7836..10586
                     /locus_tag="VF752_15565"
     CDS             7836..10586
                     /locus_tag="VF752_15565"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX8857013.1"
                     /translation="MTVAQQVNERLAALLRAGTSPWLDQIRRSLIECGELERMMREDS
                     LRGVTANPAIFEKAILGSPDYDDALADLARQDLDAESIYERLAIADVQAAADVLRPVW
                     DETGGADGFISLEVAPELARDTEGTMEQVRDYWKRLDRPNVMIKIPGTAEGMPAIEQA
                     TFEGINVNVTLLFAVEKYEQCAEAYIRGLERRREAGERVDDMHSVASFFVSRVDTEVD
                     RRLDQLGHTELCGTAALANARLAYSRFKQIFYGERFAELRDEGAFVQRPLWASTGVKN
                     PRYPETMYVDGLIAPDTVNTMPLQTLMAFGERGQVPGATADQPEEKIRETLDALAKAG
                     IDLDDVTAKLLDDGIVLFEESLHKLLAGIEKRRAAVVTGRPETIEAHFPPELERAVAD
                     RVKRAADEDVVRRIWRKDETLWAPLGTPEIANRLGWLTITEPMLEEKESIESFARECA
                     ADGLRDAVLLGMGGSSLAPEVLRESFEPKPDALRLHVLDSTDAGAILAVEREIDLENT
                     LFVVSTKSGGTIETLSLARYFLGKAPNPGRQFIAITDPGSGLVDFAESNGFRRVFLAD
                     PDIGGRYSALSHFGLVPAALAGIDIGALLERAQVAEQRCAAWEQSETNSGLWLGCALG
                     ECAVKGRDKLTFIVADRVGAFGLWVEQLVAESTGKEGKGIVPIVDEPLGEADAYGNDR
                     VFVHIRDQGNPDSAAEELVQALSEAGHPTVTVQVEEAEDLGSLFFFAEFATAVAGWVL
                     GINPFDQPNVQEAKDNTKKVLDRYASEGELPAVKDASDEALNALVTQAEPGRYFAVMG
                     FLAPSTDVDAAVEELRATVRDKSGAATTFGYGPRFLHSTGQLHKGGAPIGLFLQLLHD
                     SSEDAEVPEAGYTFNTLKNAQAVGDLETLLTHGLPAERLHLDGDPAQAIRALAERLGR
                     ML"
     gene            10586..11488
                     /gene="gnd"
                     /locus_tag="VF752_15570"
     CDS             10586..11488
                     /gene="gnd"
                     /locus_tag="VF752_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX8857014.1"
                     /translation="MQLGFVGLGRMGGNMVTRILRDSGNEIVAYDPGQSAVEAAVGHG
                     ATAATSLEDLVSKLEKPRAVWLMVPAGEITQSSLDSLLGLVDQGDLIVDGGNSKWTDS
                     RAFGERATQAGVEFVDVGTSGGVWGLEVGYCMMAGGTDEAVKRLAPILDVLAPPDGWM
                     HVGGHGAGHYVKMVHNGIEYGMLQAYAEGFEIMHASDYDLDLHGIAHLWNQGGVVRSW
                     LLELAERAFDQDGNDLTHIRGYVQDSGEGRWTVEDAINRSVPAPIITLSLLTRFASRQ
                     DESYSAKVIAALRNQFGGHAVKRE"
     gene            11493..13016
                     /gene="zwf"
                     /locus_tag="VF752_15575"
     CDS             11493..13016
                     /gene="zwf"
                     /locus_tag="VF752_15575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX8857015.1"
                     /translation="MTTVQENPLVEGLERLPVHPTTLVIFGATGDLSHRKLLPALYNL
                     AHEGALPERFNLLGLARSQMSDDEYRETARDSIMRFSRTRPDENVLNSLLERIRYLHG
                     SFDDPSVYHQLSEIADELDEEAGMPFNRIFYLATAPAFFETIAALLGNHSLDRHEGAD
                     VRIVVEKPFGHDLESAQHLNQALRRVFEERQIYRIDHYLGKETVQNILVMRFANGIFE
                     PLWNRGFVDYLQITAAEDIGIGSRAGYYDRSGALRDIVQNHLMQLLTLLCMEPPVSFD
                     ADEVRNEKVKVLHAIRPPSPDEVNEIAVRAQYGPGVAEGKEVPGYIEEEGVAPGSTTE
                     TYAALRLEVDNWRWAGVPVYLRAGKRLARRLTEIAVTLKPVPHLAFQQGGSLGVQPNQ
                     LVLTVQPNEGVSLKLVAKIPGARMRVRPVNMEFLYGTSFLSQSPEAYERLILDTMRGD
                     ATLFARDDEVEAAWALIDPILKVWSQQPGPVPTYPAGSAGPPEAEQILLPGQEWRKL"
     gene            13001..14077
                     /locus_tag="VF752_15580"
     CDS             13001..14077
                     /locus_tag="VF752_15580"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX8857016.1"
                     /translation="MEEALTTTAAVPTARWYAEDTTPAEIEAALRELLEQQHTHDAAP
                     TPARVLNLIAVVDRPWRGEIVNRLERVGRYHPSRTVVVAVQPGKRTLDAWATLGASDM
                     GDGLMVIQEQVEIELGERQLPGLAAIVDPLILSDLTTLVWSPHGHEDAVDALSGLADV
                     VLFDSLDVQDMREAVKRACRLSEDCYVVDLAWLRSTPWRERVAATFDPPRWRPALREI
                     THVAVRHRPDSAVSGLLFLGWLSSRLGWTAGALTSAGGRLSGRSRAGKQEVRVELQPV
                     PMDAPGLAGVTIETASGDSLSLDRGPGGLAAKRRTRDGLESSYVVLGASRGEPGILGE
                     GIRQALLRDPTYRPAVACAQRMLG"
     gene            14074..14775
                     /gene="pgl"
                     /locus_tag="VF752_15585"
     CDS             14074..14775
                     /gene="pgl"
                     /locus_tag="VF752_15585"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8857017.1"
                     /translation="MNKPRVEVHDDPAEAAARLLTETAQAGGHIALSGGSTPKAAYER
                     AAELGADWGTATVWFGDERCVPPDHEQSNFLMAKQALLDRLERPPAIHRMQGELGPEQ
                     GAASYEQELRGAFGDELPSLDVALMGLGPDAHTASLFPNDPALAEDTRLAVGVPVPGM
                     APLVPRITLTLPVFNAARSVVFLVHGEDKAEAVARAFSGRRSSDAPASLVDPRFGSFT
                     LLADEAAVSQLRQSA"
     gene            14772..>16696
                     /locus_tag="VF752_15590"
     CDS             14772..>16696
                     /locus_tag="VF752_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8857018.1"
                     /translation="MSGPAAAAGLSSFPTIGAYAFLSDCHTGALVAPSGAVEWLCLPR
                     MDSHSTFGALLDRDAGAFRVGPADVTVPAGWRYIPGTMVLETTWMTRTGWLVVRDTLL
                     IGPWRHEHVHESTHRRPPDDYDADHVLLRIMQCVQGTVDLGLMCEPVFDYGRARAHWE
                     YTGDSYNEAMARSEGVDLELRLTTDLRLGFEGGRCEARTTLHDGDRRFVALSWSDEEP
                     PRDVDEAGRRLDVTKDYWREWLDRGNFPDHPWRVYLQRSALTLKGLSYQPTGAICAAA
                     TTSLPESPGGERNWDYRYSWVRDSTFSLWALYTLGFDAEANDFFYFLADACGHGETPL
                     QVLYGVNGETELPERRLDHLTGYDNSRPVRSGNDAYQQDQHDVWGAALDSVYLHVKSR
                     DGLPDELWPMLRKQVETALEHWREPDRSIWEVRGEPRHFTSSKMFCWVAADRGARLAE
                     LREDVKNQERWSAAADEVKQDILANGLDSRGVFTQHYDTDALDASVLLMPLLRFLPPE
                     DERIQATVKAIAEELTEDGLVLRYRVEETDDGLRGDEGTFAICSFWLVSALCEIGELE
                     QASDLSEKLLAYASPLGLFAEEIDTRTGRHLGNFPQAFTHLALINAVMHVIRGEEQRE
                     LESSGITPAQEAPLELGT"
BASE COUNT         2649 a         5638 c         5910 g         2499 t
ORIGIN      
        1 gggcgcgccg atcatcagcg gcagcacgta gttggcgatg cctgcgaaga ccgggatgat
       61 gaacaggaag atcatcagcg aggcgtgcac cgagaacagc ccgttgaagg tgttcgagtc
      121 cacgaactgc cggcccggcg ccgcaagctc ggcgcgcatc aacatcgcca tcaggccgcc
      181 gacgatgaag aagaagaagg tggtgacgat gtactggatc ccgatgacct tgtggtccgt
      241 gttcacgcgg aagtagtccc tccacgtgta ggcgccgtgg ccggagtgat cctctggcac
      301 ggtgggcgca cccgaggccc agcggaacca gtagtcgaag cagccgatgc cgatcaggaa
      361 ggcgagcggc gtggagatga gcgccacggt ggtgatcgca tcgccgtcga cgatcggatg
      421 ccacccgtac agggcgcgga tcgggacgac gatggcgaac gcgagcccga cgccgatcgg
      481 aatggacagc gccgctcggt accagcccgg tgccctcagt ctcgctgcaa gcgtcatccg
      541 ctacctgcct ttcctcccga gacactcaaa aggtacttga cgagcgcgtc cagttgagcg
      601 ccgctgatcc ggtccttgaa gtcggtcggc atcaccggct ggaagcccgg aacgacgaac
      661 gccctggggt cgacgatcga ctgcttgacg tactgcgccg ccgtctcgtt gttctgcttg
      721 ccgaacttcg ccgcggccgc cgccaggtcg tcgagcttcg gcccgaccgc gccgctcgtg
      781 ccggcatccg acagcgtgtg gcagccgccg cagccgtttg aggcgaacac ctgcttgccg
      841 gccgcgttcg cgttgccacc gccgccgccc tgaggcgccg cgccgcccgc gggcgcctgg
      901 gcctgctgct gcggaccccg ggcctgcctc gcgagccatg cagtgaacgc cggcttctcg
      961 accacgcgca ccgtgttccg catcgtcgag tgcccgagcc cgcagagctc cgcgcacacg
     1021 acgtcgtagg tgccgacctt gttgggcgtg atcttgatgt gggtcgtcat cccgggtacc
     1081 gcgtcctgct tgagccggaa ctgcggaacc cagaacgagt ggatcacgtc gttggtggtg
     1141 atgtcgaact tcacccgctc cttgttgggc aggtaaagca cgtcggaggt gaccggtttg
     1201 ccgttcgggc cctcgtaggt gaagtgccag gtgaactgct ggccgcggac cttcacggtc
     1261 atcgccgcgg ggctcgcctc cacgttgtgg agcacgaccc aaccgtaggc ggcgagcgcg
     1321 gtcacgagca ggaacgggat gctcacccag atgatctcga gccgcgtgtt gccgtggatc
     1381 ggcgcgccgt cgctcaggtc gcccggcttc gcccggaact tcacgacgct gtagatcgcg
     1441 acggtcatga cgagcacgaa gatcggcacc gacacgatca ggagcacgtc gtagagcgtg
     1501 tcgatctcgc cgaccttgtc ggccgcgagc ggcgggaacc agtcgatgga gagcgcgatc
     1561 gcgacgccga tgaccgacgc cacggcgcca atcaagagca tctgggccag cggatgctta
     1621 cgcaatctct cccgtccccc ggagcatcgg ggcgcgagtg taatcgacca tcgcgacgaa
     1681 cgcctaccag cgttcgatcg cggacggagc ggctctcaga cggcggccac cgcggcctcg
     1741 ccagggcgga agctcgacgg tggagcgccg tgatggcggc ggaacgtctt ggcgaactgc
     1801 gcgggctgcc ggtagccgac gctcatcgcc acctggcgga ctggcatgcg tcccgtccgc
     1861 agcagctcca tcgcccggcg catccgcacg tcggccagat acgcgcggaa cgtcgtgttc
     1921 ccggcctccg cgaaggcgcg ctggagctgg cgcctcgacg tggcgatccg ctgcgccacc
     1981 tggtccaggt ccagctcctg ggagtagtcc tcttcgatga tcgcgaccgc ctcctcgaaa
     2041 agagctctgc ggcggtcgac cgtcgcttgc cgcttcgcca atctctccct ttcagttccg
     2101 acttacggga gaaccggccc ttcagccctg gcgctcccgc cccgggatgt tatgcccgag
     2161 ccacggctcg acctatcccg ttgtcagcgc tcgaacgcgc gaaattttgc acttgcaggg
     2221 gctttgtccg cttcggctgg gggaacaggc ggctgtgaga ccattgggat acgcttgcgt
     2281 cattccgcac gagtcggttc ggaggagggc aggagacgag atgcaggtca gcgatgggat
     2341 gagctcggtg atcctcacgg tgggcccgga tcacacaatc cgcgacgcgg cgcgccagat
     2401 ggcggagcgc aaggtcggcg cggccatcgt catcgacccg gaccagccgg gaccgggcat
     2461 cgtgaccgag cgcgacatcc tcgaatcgat cgcggccggc cagagtccag accaggagcg
     2521 ggtcgctaac catctgtccg cgaatctcac gttcgccgca tccgactggt ccctcgagcg
     2581 cgccgccgag gcgatggtga agggtggctt ccgccatctg gtcgtaatgg acggcggcga
     2641 gaccgtcggc atcctgtcca tgcgcgacat cgtgcgctgc tggaccatgg atggggccac
     2701 gtgcgacgtc tgatctgacg gctgcaaacc ggcgctgggg ggcgtcctcg gtcggccgca
     2761 tgcagagcat gcgcgcctcc ctgcgtcctt ccccagcttg gttttcgctc gtcagatcag
     2821 gcgtcggctc gcgctaggcg cggtagcggg ccttctccct cattgccctc gacctcgagc
     2881 taccgatacg acgcgaagcg cagtaggtcc gccagctcga agttcccgtt caccgagggc
     2941 agggtcggcc gccattccgg gtccaccacc cgctgtgagt ccgggtccgc gtcgatgatc
     3001 ccgagcagca cctcggccac tatctggccg ccaaccggcc cgaggcgttc gccgtcctgc
     3061 cgcacctcgg cctccttcag gacgtagagc cagagcggcg tctcgccgct ccagccggag
     3121 tccgcgagtc ccacctgctc gcgcgtgagc ggctgctcgc ccatcgcccg cgcgaccgac
     3181 tcgcccgacg gcagcgaaag cgcatgcccg cgctggaggt cgcgcaccgc cagcgagtgg
     3241 tactcctcga ccgcaacgtc gccggtgatc tcgagcggca gctcgattag cgagcgcgcc
     3301 aggcggccgt cgatcttctt ggccggctgc ggcggctcgg ctcccggcat ctcgaacagg
     3361 tgcgaccagt cgacctcgcg cgctgccggc accgggcgga agcccatcag atccgggaac
     3421 agcggcaggt ccccggagaa gtcgttcagc cggtacgagt gccggatctg gccgtggccg
     3481 tagcggtagg ccccgtccgc gaactcgaac gggatgaacg cgtcgccctg cggccggtag
     3541 aagcgcgggc cgctgtcgag cagcgagcgc atcaggtccg cgccgaccag gcgaggcagg
     3601 aagtcgtgga cgatcaccca ctggtagtgc cagtgcgtgg cgcgggctgc ctcgtcgaag
     3661 agctcgtcct cggctactcc gtcttcgcgc aggcggtcca ccagcccgtt gtgcaccttg
     3721 aggaaggcga ggtgcagctg gctcacgaag agatggacgt cgttgcgcgg gtcgccgatg
     3781 agcgcggtgc cctggcggtt gcgcggcacg tcggccggct cgccctcgtc gttctccccg
     3841 atcagcagct tcggcgcgtc gtcgcggtcg tagaggtacg ggcttccgac ctggcccgcg
     3901 ccgtagaggc actcgaggtt ggcgcgcggc gtgcggaagt tgcggacgct gtccgggtcg
     3961 gcatgctcct ggaggctcga gcgatcggcc gttatgtcgt gcgcgatgaa ctggccgaac
     4021 agcggccagc cggccgcgcc gcgggcgtcg tcacagcccg ggtcgtcggc catcgcgctg
     4081 ccgtcgcaga agccgccgct gcgcccgagc gcgagcacga gcggctcctc ttcggagacc
     4141 ggcggcagct ccgggaacat gcgaccgtac ttgctcgcgt tggcgagcgg cgcgtcgatc
     4201 gtgcgcgacg gtgcgaggca gtggtcgcgc gcttgtgcag ttgtcgcggt gagggccata
     4261 gccccaagct acgcgcacgg tcaaggtggc tcacgccacc cgatcgggtc accgcgtggt
     4321 cagtgttcga gcggtagctc tgagatgtcc tcgcgcgtcg agcgtatcca gcgcacgagc
     4381 acgacgagga agatcacgag gccgatgccg gtgaggaccc acgtgtagac gacgccgacc
     4441 agcatcagcg tgatggcgaa cgccatcagc acggggaggt aggacggccc ggggagatgg
     4501 atctcctcac cgggcggtgg cacgttttcg tgcgcggata cttcttgctg ctcggccatc
     4561 tcagtagacg taggccgcca caaatgccag cgcgaagacg atgaggatga tcgccccggc
     4621 gatcagggcg gtgcgaatgt tctgcagcgc taggcggcgg tccatgcggg tccctttgta
     4681 acagatcggg gtcgcgagac gctgacacgg gtatgcggcc cgaatccccg ctcagcgcga
     4741 ccgctaaggt gccgtgcgga gtcgagtgag cccatgtcac cagtcactgt aggcgtcgat
     4801 gtgggcggca caaaggtggc cgccgcggcg gtcgagggca cgaaggcact cgaccggctc
     4861 gagcgaccca cggatctgaa cagctcgggc gcggtcgtcg agcagatcga ggcggtggcg
     4921 cgtgaggcgc tgtcgcacgc gcccgaggcg gaggcggtcg gcgtcggcgt gccctcccag
     4981 atcgacttcg agaccggcga ggtgatcgcg agcgtgaaca tcccgcttcg tggcgtccgc
     5041 ctgcgcgagg agctcgagcg caagctgaac ctgcccgtgt tcgtggacaa cgacggcaac
     5101 gtcgccgcga tggccgagct gcacctgttc gacgagcccg acatccggca cctgatcatg
     5161 ctcacgctcg gcaccggcgt gggcggcggc gtggtgatcg accgccacat cttccgcggc
     5221 gcgaccggcc gcggcgctga gctcggtcac atcgttgtac aggcagatgg gcccgaatgc
     5281 ccgggcgatt gcccgaacca cggctgtctc gaggcgtact gcagcggcct cgcgctagag
     5341 cgggatgcga ccgcgctagg caaggaaaag cccgactcgg ggctcggcag ggttgtgcgc
     5401 gagcgggacg ggcgctgccg cggccgcgat gcggtcgagc tcgcacgaga gggcgatctg
     5461 gaagccgttc agcttctcca gcgcttgggc acctacctcg gtgtggggat gacgagcctg
     5521 atgaacgcct tcgagccgga gatgatcgtg atcgggggcg gcctcgggca ggcggccgac
     5581 cttttcctca accgcgcgat cgaggagacg aagtcgcgcg tgctgcccgc gctcgagccg
     5641 cgagtgcggg tcgagcgcgc gctcggcggc cccgacgcgg gcgtgatcgg cgccggcctg
     5701 ctcgccgccc acgagcacaa gcgcgcgggt ggacatactc cagaggaaac gatcaaggaa
     5761 ggggcgctgt gaccacgagc acgacggaca cgcggacgct cgacgagctc tccgtgaaca
     5821 cgatccgcac ccttgccatg gacgccgtcc agaaggccaa ctccggccac cctggcaccc
     5881 cgatggcgct cgccccgctc gcatacgtgc tctacacgcg cgtgatgaag cacgaccccg
     5941 ccgatcccga gtggttcgac cgcgaccgct tcgtgctgtc ggccgggcac gcctcgatgc
     6001 tgctctacgc ctcgctctac ctctcgggct acgggctcga ggtcgaagac ctcgagaact
     6061 tccggcaggt cggcagcccc accgccggcc accccgagta ctggcacgcg aagggaatcg
     6121 agaccaccac cgggccgctc gggcagggca tctccacctc ggtcggcatg gcgctggccg
     6181 agcgcatgct gaacgcgcgc tttggcgacg aggtgatcga ccaccacacc tgggtgatcg
     6241 cgagcgatgg cgacctcgag gaggggatcg cctcggaggc gtgctcgctc gccgggcacc
     6301 tcggcctcgg tcggctgatc gtcttctacg acgacaacca catttcgatc gagggcgaca
     6361 ccgagctcgc cttcaccgag gacgtgggca agcgcttcga ggcctatggc tggcacgtga
     6421 tcaacctcgg cgagcagatc gatctcgagt cgctcgagcg cgccgctcac caggcgaagg
     6481 ccgtcgagga ccgcccgtcg ctggtgattg tgcgcacgca catcgcgcct ggcagcccca
     6541 acaagcaggg atcggcgaag gcgcacggcg agccgctcgg cgtcgaggag gtccggctga
     6601 ccaaggaggt ctacggctgg ccctcggaca agaccttcta cgttccggac gaggtgctcg
     6661 agcacttccg cagtgtcacc gcgaagcgcg ggcgcgaggc gcacgagcag tggaacagtc
     6721 gcttcgagga gtaccgacgg cagcaaccgg agaggtcgga cgagctcgag ctgttcatcg
     6781 agcgacggct gccggacggc tgggacagcg acgtgccgcg cttcgacccc aatgacgaga
     6841 aggtcgggtc gaagctcgcg acgagaaagg cttcgaacgc ggtgatccag tgggccgcgg
     6901 cccgcgtgcc gcaactggtg gggggctcgg ccgacctcgc gccgtccacg ctcacgctga
     6961 tcgacggcgg cggcgatgtc gagaaggggc actacggcgg gcgcaacatg cacttcgggg
     7021 tgcgtgagca cgccatgggc gcgatcgtga atggcctcgt gctgcacggg ttccgggcct
     7081 tcggcgccac cttcctgatc ttctcggact acatgcgcgg ttccatcagg ctgtccgccc
     7141 tgatgcagat cccgtcgatc ttcgtattca cgcacgactc gatcgggctc ggggaggacg
     7201 gcccgacgca ccagccgatc gagcagctcc ccgggctgcg tgcggtaccg aacctctacg
     7261 tcgtgcgtcc cgccggcgcg aacgagaccg cgcgcgcgtg ggagttcgcc ctgcggcaga
     7321 ccaaccggcc gtgcgcgttc gcgctcacgc gccagggcgt gcctgtctgg gatcccgacg
     7381 gcgtgcccgc cgactgcgtg gagcgcggcg cctacgtgct gcgtgacgcc tccgacgggc
     7441 gcgagcccga ggtgattctg atcggcacgg gctctgaggt gcacctctgc gtggaggcgc
     7501 agacgctgct cgaggcggac ggcgtggcga cccgcgtggt gagcatgccg tgcgtcgagc
     7561 gcttcctcga acaggacgag tcgtaccgcg accaggtgct gccggggtcc gtgcgggcgc
     7621 gcgtggctgt ggaggcggcg cacccgcaga cgtggcacca gttcgtcggc gaggccggcg
     7681 ccatcgtcgg catggagacg ttcggcgcct cgggcccgga caaggcggtg ttccagcact
     7741 tcggattcac accagagcgc gtggccgacg aggcccgcgc cgtaatcggc cgcctgtcgt
     7801 cggcgccggc gggcggctga gagaggaggc acgccgtgac cgttgcccag caggtgaacg
     7861 agagactcgc ggcgctcctc cgagccggca cgagcccctg gctcgaccag atacggcgca
     7921 gcctcatcga gtgtggtgag ctcgagcgca tgatgcgaga ggactcgttg cgcggcgtga
     7981 ccgcgaaccc ggccatcttc gagaaggcga tcctcggcag cccggactac gacgacgcgc
     8041 tcgccgacct cgcgcgccag gacctcgatg ccgagtcgat ctacgagcgc ctcgcgatcg
     8101 cggacgttca ggcggcggcg gacgtgctcc gcccggtctg ggacgagacc ggcggcgccg
     8161 acgggttcat ctccctcgag gtggcgcccg agctcgctcg cgacaccgag ggcacgatgg
     8221 agcaggtgcg cgactactgg aagcggctcg accgcccgaa cgtgatgatc aagatcccgg
     8281 gcaccgccga ggggatgccc gcgatcgagc aggccacgtt cgaggggatc aacgtgaacg
     8341 tgacgctgct gttcgcggtg gagaagtacg agcagtgcgc cgaggcctac atccgcggcc
     8401 tcgagcgccg gcgcgaggca ggcgagcggg tcgatgacat gcactcggtg gcgagcttct
     8461 tcgtgtcgcg tgtcgacacg gaggtggacc gccgcctgga ccagctcggc cacaccgagc
     8521 tgtgcggcac ggcggcgctc gcgaacgcgc ggctcgccta cagtcgcttc aagcagatct
     8581 tctacgggga gcgcttcgcg gagctgcgcg acgagggcgc gttcgtgcag cggccgctct
     8641 gggcctcgac gggagtcaag aacccgcgct atcccgagac tatgtacgta gacgggctga
     8701 tcgcgccgga caccgtcaac acgatgccgc tgcagacgct gatggcgttc ggcgagcgcg
     8761 gtcaggtgcc gggcgccacg gccgaccagc ccgaggagaa gatccgggag acgctcgacg
     8821 cgctcgccaa ggctgggatc gatctcgacg acgtgaccgc gaagctgctg gacgacggca
     8881 tcgtgctgtt cgaggagtcg ttgcacaagc tcttggccgg catcgagaag cggcgcgccg
     8941 ccgtggtcac cgggcgtccc gagacgatcg aagcgcactt cccgcccgag ctcgagcggg
     9001 cggtcgccga ccgcgtcaag cgggccgccg acgaggacgt cgtccgcagg atctggcgta
     9061 aggacgagac gctgtgggcg cccctcggca cgcccgagat cgccaaccgg ctgggctggc
     9121 tcacgatcac cgagccgatg ctcgaggaga aggagtcgat cgagtcattc gcgcgcgagt
     9181 gcgccgccga cgggctgcgc gacgccgtgc tgctcggcat gggcggctcg agcctcgcgc
     9241 ccgaggtgct gcgcgagagc ttcgagccga agccggacgc gctgcggctg cacgtgctcg
     9301 actcgaccga cgccggcgcg atccttgcgg tcgagcgcga gatcgacctc gagaacacgc
     9361 tcttcgtggt ctctacgaag tcgggcggaa cgatcgagac gctctcgctc gccaggtact
     9421 tcctcggcaa ggcgcccaat ccgggccgtc agttcatagc gatcaccgac ccgggctcgg
     9481 gcctcgtgga cttcgcggag tcgaacggct tccgccgagt cttcctggcc gacccggaca
     9541 tcggcggccg ctactcggcg ctgtcgcact tcggcctggt gccggccgcg ctggcgggca
     9601 tcgacatcgg tgcgctgctc gagcgtgcgc aggtggccga gcagcgctgc gcggcctggg
     9661 agcagagcga gaccaactcg gggctctggc tcgggtgcgc actcggcgag tgcgcggtca
     9721 aaggacgcga caagctcacc ttcatcgtgg ccgaccgcgt gggggcgttt ggcctctggg
     9781 tggagcagct cgtggcggag tccaccggca aggagggcaa gggcatcgtt ccgatcgtcg
     9841 acgagccgct cggggaggcc gacgcttacg gcaacgatcg cgtgttcgtg cacatccgtg
     9901 accagggcaa tcccgactcg gccgcggagg agctggtgca ggcgctcagc gaggccggtc
     9961 acccgaccgt gacggtgcag gtcgaggagg ccgaagacct aggcagcctg ttcttcttcg
    10021 cggagttcgc gaccgctgtg gcaggctggg tgctcggcat caaccccttc gaccagccga
    10081 acgtgcagga ggcgaaggac aacacgaaga aggtgctcga ccgctacgcg tccgagggcg
    10141 agctgcctgc ggtgaaggac gcctctgacg aagcgctgaa tgcgctcgtg acgcaggcgg
    10201 agcccggccg ctacttcgcc gtaatgggct tcctcgcgcc gtccaccgat gtggacgcgg
    10261 cggtggagga gctccgggcc accgtgcgtg acaagagcgg cgcggcgacc acattcgggt
    10321 atgggccgcg cttcctgcac tccacgggcc agctgcacaa gggcggcgcg ccgatcggcc
    10381 tattcctcca gctgctgcac gactcctccg aggacgcgga ggtgccggag gccggctaca
    10441 cgttcaacac actgaagaac gcgcaggcgg ttggcgacct cgaaacgctg ctcacccacg
    10501 gtctcccggc ggagcgcctc cacctggatg gcgatcccgc acaggcgata cgagcgctcg
    10561 cggagcggct cgggaggatg ctctgatgca gctcgggttc gtcgggctcg ggcgcatggg
    10621 gggcaacatg gtgacccgca tcctgcggga ctcgggcaac gagatcgtgg cgtacgaccc
    10681 gggccagtca gcggtcgagg cggccgtcgg gcacggcgcg accgccgcga cctcgctcga
    10741 ggacctcgtg tcgaagctcg agaagccccg agccgtgtgg ttgatggtgc ccgcgggcga
    10801 gatcacccag agctcactcg attcgctgct cgggctggtg gatcagggtg acctgatcgt
    10861 ggacggcggc aactcgaagt ggacggactc aagggccttc ggcgagcgtg ccacacaggc
    10921 gggagtcgag ttcgtcgacg tcggcacgag cggcggcgtc tggggcctcg aggtcggcta
    10981 ctgcatgatg gccgggggca ccgacgaggc cgtgaaacgg ctggcgccga tcctcgacgt
    11041 cctggccccg ccggacgggt ggatgcacgt gggcggccac ggcgcgggcc actacgtgaa
    11101 gatggtccac aacggcatcg agtacgggat gctccaggcc tacgcggagg gcttcgagat
    11161 catgcatgcc tcggactacg acctcgacct tcacggcata gctcacttgt ggaaccaggg
    11221 cggcgtggtc cgctcgtggc ttctcgagct ggccgagcgc gccttcgatc aggacggcaa
    11281 cgacctgacg cacatccgtg gctacgtgca ggactcgggc gagggtcgct ggacggtcga
    11341 ggacgcgatc aaccgaagcg tgccggcgcc gatcatcacg ctgtctctct tgacgcgatt
    11401 cgcctcccgc caggacgagt cgtactcggc gaaggtcatc gccgcgctcc gcaaccaatt
    11461 cggcgggcac gccgtgaagc gcgagtagcc gtatgaccac ggtgcaggag aacccactcg
    11521 tagaagggct cgagcggctc cctgtccacc cgaccacgct cgtgatcttc ggcgcaacgg
    11581 gcgacctctc gcaccgcaag ctcctgccgg ctctctacaa cctcgcgcac gaaggcgccc
    11641 ttcccgagcg cttcaacctc ctgggcctcg cgcgcagcca gatgagcgac gacgagtacc
    11701 gggagaccgc gcgcgactcg atcatgcgct tctcgcgcac gcgccccgac gagaacgtcc
    11761 tgaactcgct gctcgagcgc atccgctacc tgcacggctc cttcgacgac ccgtcggtgt
    11821 accaccagct ctccgagatc gccgacgagc tcgacgaaga ggcgggcatg ccattcaacc
    11881 gcatcttcta cctcgctacc gcgccggcct tcttcgagac gatcgcggcc ctgctcggca
    11941 accactcgct cgaccggcac gagggcgccg acgtgcgcat cgtggtcgag aagccgttcg
    12001 gccacgacct cgagtccgcg cagcatctga accaagcgct gcggagggtc ttcgaggagc
    12061 gccagatcta ccgcatcgac cactacctgg gcaaggagac ggtccagaac atcctggtga
    12121 tgcggttcgc caacggaatc ttcgaaccgc tctggaatcg gggcttcgtc gactacctcc
    12181 agatcaccgc ggcggaggac atcgggatcg ggtcgcgcgc gggctactac gaccgctcgg
    12241 gtgcgctacg cgacatcgtg cagaatcacc tgatgcagct cctgacgctg ctctgcatgg
    12301 agccgccggt gtcgttcgat gccgacgagg tacgcaatga gaaggtgaag gtgctgcatg
    12361 cgatccggcc gccatcgccc gacgaggtga acgagatcgc cgtgcgcgcg cagtacgggc
    12421 ccggcgtggc ggagggcaag gaggtgccgg gctacatcga ggaggagggt gtggctcccg
    12481 gctcgaccac ggagacctac gcggcgctgc gcctcgaggt cgataactgg cgctgggccg
    12541 gcgtgccggt gtacctgcgc gcgggcaagc ggctcgctcg gcggctcacc gagatcgcag
    12601 tcacgctcaa gccggttccg cacctcgcct tccagcaggg cgggtcgctc ggcgtgcagc
    12661 cgaaccagct cgtgctcacg gtccagccga acgagggagt ctcgctcaag ctcgtcgcga
    12721 agatccccgg agcacgcatg cgggtgcgtc cggtgaacat ggagtttctg tacggcacct
    12781 cgttcctctc gcagtcgcca gaggcgtatg agcggctgat cctcgacacg atgcgaggcg
    12841 acgccacgct gttcgcgcgc gacgacgagg tcgaggcggc gtgggcgctc atcgacccga
    12901 tcctgaaggt gtggtcgcaa cagcccgggc cggtgccgac gtacccggcg ggctccgccg
    12961 gcccgcccga ggccgagcag atcctgctgc cgggccagga gtggaggaag ctctgaccac
    13021 cacggcggcc gttccgaccg cccgctggta cgcagaggac accacccctg cggagatcga
    13081 ggccgccctg cgcgagctgc tcgagcagca gcacacgcac gacgccgcac caacgcccgc
    13141 ccgggtgctg aacctgatcg cggtcgtgga ccgcccgtgg cgcggcgaga tcgtcaaccg
    13201 cctcgagcgc gtcgggcgct accacccgtc gcgcacggtg gtcgtggcgg tgcagccggg
    13261 caagcgcacg ctcgatgcct gggccacgct cggcgcgagc gacatggggg acgggctgat
    13321 ggtcatccag gagcaggtcg agatcgagct cggcgagcgg cagctgccgg gtctggccgc
    13381 gatcgtggat ccgctcatct tgtccgatct gaccacgctc gtgtggtcgc cgcacgggca
    13441 cgaagacgcc gtcgatgcgt tgagcgggct cgcggacgtg gtgctgttcg actcgctcga
    13501 cgtgcaggac atgcgggagg cggtgaagcg ggcgtgccgc ctcagcgagg actgctacgt
    13561 ggtcgacctc gcctggctcc gctcaacgcc gtggcgcgag cgggtggcgg ccaccttcga
    13621 cccgccgcgc tggcgcccgg cgctgcgcga gatcacgcac gtggcggtgc ggcaccggcc
    13681 cgactcggcc gtgagcgggc tcttgttcct cggctggctc tcctcgcgcc tcggctggac
    13741 agccggcgcg ctcaccagcg ccggcgggcg gctctcgggc cgctcccgcg cgggaaagca
    13801 ggaggtgcgg gtggagctcc agcccgtgcc gatggacgcg cccggcctcg ccggggtcac
    13861 gatcgagacc gcttccggcg actcgctctc actcgaccgc ggtcctggcg ggctcgcggc
    13921 gaagcggcgg acgcgagacg ggctcgagtc gagctacgtg gtgctcggcg cgtcgcgcgg
    13981 cgagccgggg atcctcgggg agggcatccg ccaggcgctg ctgcgcgacc ccacctaccg
    14041 gccggcggtc gcatgcgcgc agaggatgct cgggtgaaca agccgcgcgt ggaggtgcac
    14101 gacgaccccg cggaggcggc agcgcggctc ctgaccgaga ccgcgcaggc cggcgggcac
    14161 atcgccctgt ccggcggctc cactccgaaa gcggcgtacg aacgggcagc ggagctcggg
    14221 gccgactggg gcacggccac cgtgtggttc ggcgacgagc gctgcgtgcc gcccgaccac
    14281 gagcaatcga acttcctcat ggcgaagcag gcgctgctcg accggctcga gcggccgccc
    14341 gcgatccacc ggatgcaggg cgagctcggg ccggagcagg gcgctgcttc ctacgagcag
    14401 gagctgcgcg gcgcgttcgg cgacgagctt ccgagcctgg acgtggcgtt gatgggcctc
    14461 ggcccggatg cccacacggc gtcgctgttc ccgaacgatc ccgcactcgc tgaggacact
    14521 cggctcgctg tcggcgtgcc cgtgcccggt atggctccgc tggtgccgcg catcacgctc
    14581 acactccccg tcttcaacgc ggcacgatcg gtcgtcttcc tggtgcacgg cgaggacaag
    14641 gcagaggctg tggcacgtgc cttctccggg cggcgctcga gcgatgcgcc ggcgagcttg
    14701 gtggatccgc gcttcggatc cttcactctg ctggccgacg aggcggcggt gagtcagctg
    14761 aggcagtctg cgtgagcggt ccggccgcgg ccgccgggct cagctcattc ccgacgatcg
    14821 gcgcgtacgc attcctctcc gactgccaca cgggcgcgct ggtggcgccg agcggcgctg
    14881 tcgagtggct ctgcctaccg cgcatggaca gccacagcac cttcggggcg ctgcttgatc
    14941 gggacgccgg cgcgttccgc gtcggccccg ccgacgtgac cgtgccggcc gggtggcgct
    15001 acatcccggg caccatggtg ctcgagacca cctggatgac gcggacgggc tggctcgtcg
    15061 tacgcgacac gctcctgatc ggaccctggc ggcacgagca tgtgcacgag agcacgcacc
    15121 gccggccgcc cgacgactac gacgccgacc atgtcctgct ccggatcatg cagtgcgtac
    15181 agggaacggt cgacttgggc ctgatgtgcg agccggtgtt cgactacggc cgcgcgcgtg
    15241 cgcactggga gtacacggga gacagttaca acgaggcgat ggcgcgcagc gagggcgtcg
    15301 acctcgagct gcggctcacg acggatctgc gcctgggctt cgagggtggc cgctgcgagg
    15361 cgcgaaccac gctgcacgac ggcgatcgcc gcttcgtcgc gctgtcgtgg agcgacgagg
    15421 agccgccgcg tgacgtcgac gaggcgggcc gccgcctcga cgtgaccaag gactactggc
    15481 gcgaatggct cgaccgcgga aacttccccg accacccgtg gcgggtgtat ctccagcgaa
    15541 gcgcgctcac gctcaaggga ttgagctacc agccgactgg cgcaatctgc gccgcggcga
    15601 ccacgtcgtt gccggagtcg cccgggggcg agcgtaattg ggattaccgc tacagctggg
    15661 tccgcgactc gaccttctcc ctgtgggcgc tttacacgtt ggggttcgac gccgaggcga
    15721 acgatttctt ctacttcctc gcggacgcgt gcggtcacgg cgagaccccg ctgcaggtgc
    15781 tctacggggt gaacggcgag acggagctgc ccgagcgccg cttggatcac ctaacgggtt
    15841 acgacaactc ccgtcccgtg cggtcgggca acgacgccta ccagcaggac cagcacgacg
    15901 tgtggggcgc cgcgctcgac tcggtgtacc tgcacgtgaa gtcgcgcgac ggccttcccg
    15961 acgagctctg gccgatgctg cgcaagcagg tggagaccgc gctcgaacac tggcgcgaac
    16021 ctgatcgcag catctgggag gtacgcggcg agccgaggca cttcacttct tcgaagatgt
    16081 tctgctgggt ggcggccgat cggggcgcac ggctcgcgga gctccgggag gacgtcaaga
    16141 accaggagcg ctggagcgcc gcagcagacg aggtcaagca ggacattctc gccaacgggc
    16201 tggactcgcg cggcgtcttc acccagcact acgacaccga cgcgctcgac gcgtcggtgc
    16261 tgctgatgcc gctgctgcgc ttcctcccgc ccgaggacga gcgcatccag gccacggtca
    16321 aggcgatcgc ggaggagctc accgaggacg ggctcgtgct gcgctaccgg gtcgaggaga
    16381 ccgatgacgg cctgcgcggg gacgagggca cgttcgccat ctgctcgttc tggctggtct
    16441 cggcgctgtg cgagatcggc gagctcgagc aggcgagcga cctgagcgag aagctgctcg
    16501 cctacgccag cccgctgggg ctgttcgcgg aggagatcga cacgcggacc ggccgccacc
    16561 tgggcaactt cccgcaggcc ttcacgcacc tggcgctcat caacgcggtc atgcacgtga
    16621 tccgcgggga ggagcagcgc gagctcgaga gctccggcat cacgccggcc caggaggccc
    16681 cgctcgagct aggaac
//
LOCUS       DASVXS010000277        12645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1046252, whole genome shotgun sequence.
ACCESSION   DASVXS010000277 DASVXS010000000
VERSION     DASVXS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046252"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VF752_15595"
     CDS             <1..713
                     /locus_tag="VF752_15595"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8857019.1"
                     /translation="AFRDAGRRLAGARDAKVLIETLDTVEEAAGDELRSGSTAGLRVE
                     LESEHEAALEALRDGGATAAVQSDLEHARARVATWTLAMDGFDAIEPGLRRIYRRGRN
                     ALEAVAEEQSTENRHEWRTRVKDLWHATQIVRPASPSSMKKLSRRLHDLSDLLGDDHD
                     LAVLCTYVARHPRCFEDREEQAALVAVIERRRELLQGKALSLGRTLYEQSPKDFTRSL
                     SRPWQKRMGKPRRPAAAA"
     gene            complement(773..1924)
                     /locus_tag="VF752_15600"
     CDS             complement(773..1924)
                     /locus_tag="VF752_15600"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX8857020.1"
                     /translation="MPTQEQIRDSLRAVVDPELKKDIVELGMVRSIDAADDGTVSVVV
                     SLTTPGCPIRSHFQKAVTEQVRALEGVRSVRVDFDVLSAKEKQGLQQRVQRSTLPTGA
                     LAQVNNIICVASGKGGVGKSSMTANLTAALFAEGKTAAALDADVWGYSIPRMMGVSGR
                     PKVSAERKIIPLESHGGIKTMSIQYFLEDADQAVTWRGPMLHKAIRQFLEDVDWGELD
                     YLLIDLPPGTGDVTMTLSQLLPQARFVIVTTPQPAAQKVAQRAAQTALRFDLEILGVI
                     ENMSGFTTPTGERFTIFGEGGGQLLADDLDVPLLGKVPLQEELRVGADTGMPIVIDDP
                     SAPASQAILHAARGIIAATPQSVPVVPLPTAKPGAGNGGVPGMALPMAP"
     gene            complement(2084..2572)
                     /locus_tag="VF752_15605"
     CDS             complement(2084..2572)
                     /locus_tag="VF752_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857021.1"
                     /translation="MATQTQTKRRATAKKGAATRQRSTARRSASSTRSNARRTTKSAA
                     STATQRAKAEATGLQAVALQAERAVLVPVGAALTARDSIVENVKPYTSRETAQREISR
                     LQRRVEVNLRKFERRGTTARNQLERDLKRNRTKVERFVRRNRRQVESQAENLVSNVSN
                     LV"
     gene            complement(2709..3764)
                     /locus_tag="VF752_15610"
     CDS             complement(2709..3764)
                     /locus_tag="VF752_15610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX8857022.1"
                     /translation="MVDWSIARQVARLAAGTEPPPGKAAGADFAGMSANAERLVADYT
                     GLKLVEPVPAAEPVGRRQWSEINIQTLQGFLDPVAERMSGRLEKTGPFAGALRAAAGA
                     TLAAEVGIIAGYMSQRVLGQYEVSLLQPDASARLLYVVPNLGHAIGELGVDADAFLAW
                     IALHEMTHVFEFSGVPWLRGYMAGLLREYLHSVEVRIDSGGAGALPRLPAIAELVDQF
                     REGGLVALVQTSEQRDLMRRLQAAMSVIEGYSEHVMDAVGGEHLENYAGLRDAMERRR
                     ANRTAPQRLLEKLLGLDLKMRQYDLGKRFCDGVAARGGIELLNRAWESPESLPHLGEL
                     SDPGAWVDRIQGSRAAA"
     gene            3804..4319
                     /locus_tag="VF752_15615"
     CDS             3804..4319
                     /locus_tag="VF752_15615"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX8857023.1"
                     /translation="MTLEAHEEQHRAAARNGDTAERRDPEPVDYAEINAVYALLAIAV
                     IAVTRDRVREDPIRGYELPQLGAATFALSKVIAREKIGTWLREPFVEEADGDRRPRGK
                     RLRRAMGELVTCTRCVGAWSALGLVWLRLYSPDAGRVATAVLATSAANDWIQAGFAWL
                     TGNANETKASR"
     gene            4358..4540
                     /locus_tag="VF752_15620"
     CDS             4358..4540
                     /locus_tag="VF752_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857024.1"
                     /translation="MPTLFRYLMARRFLARYLGKRAVRFLPGGWVTFIAYPLARWAWR
                     RRMRRARARSLAARAG"
     gene            complement(4520..5443)
                     /gene="ligD"
                     /locus_tag="VF752_15625"
     CDS             complement(4520..5443)
                     /gene="ligD"
                     /locus_tag="VF752_15625"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8857025.1"
                     /translation="MAKSNSVEVEVDGRTLKLSNLEKVLYPKVGFTKGQVIDYYTRIA
                     PVLLPHLRDRPLTLKRYPNGVDDKFFYEKQCPKHRPDWVETAPIKARNKTIDFCLCNE
                     LPTLVWLANLADLELHTSLSKYDPIEQPTTLAFDLDPGPPATIVECSRVALLLRDVFD
                     SLGIEAFPKTSGSKGMQVYVPLNTDVTYDQTKPFAKALAELLEKQHPDLVVSSMKKEL
                     RPHKVLVDWSQNDEHKTTVNVYSLRAKERPTVSTPISWDEVESTERSGDPDELVFDSD
                     AALERVERDGDLFAAVVELEQELPALTGARG"
     gene            5503..5811
                     /locus_tag="VF752_15630"
     CDS             5503..5811
                     /locus_tag="VF752_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857026.1"
                     /translation="MSSGSWDAVQPARASRFARLSATLRSLAWLGITLVVLVAGVIHW
                     SDDRSADFAGAQMACAMAAALFALLGAAYTARGRRHSAVLALVLSVAAAGGWVALLFV
                     "
     gene            complement(5808..7280)
                     /gene="ligD"
                     /locus_tag="VF752_15635"
     CDS             complement(5808..7280)
                     /gene="ligD"
                     /locus_tag="VF752_15635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8857027.1"
                     /translation="MAKKLGEYKRKRDFEETPEPSGNGDSGGQPGGRFVVHEHHARSL
                     HWDLRLERDGVLVSWAIPKGIPPDPKTNHLAVHVEDHPIEYIDFKGEIPEGNYGAGEV
                     KIWDQGTYDVLKWRDKEVMVVFHGERLQGKYVLFPTKGKNWMIHRMDPPADEGREPMP
                     RDIKPMMARLAERVPDKENEWGYEVKWDGVRAIAYIEGGRVRLQNRNMRDITQQYPEL
                     RELGRAVGARELVLDGEVIAFDEEGRPSFGRLQHRMHLASESAVRRRMSDIPVVYAIF
                     DVLYLDGRVTIRLPYEERRGLLDELELRGSHWQTPSWHRGEGKALLDAAREQGLEGIV
                     AKKLDSPYEPGRRSVCWRKIKAVQRQELVVGGYMPGEGGRTGRVGSLCVGYYNMTRAE
                     AEERGEEQRLIYAGNVGTGFKEADLVKLGKLLEPLRRDTSPFAGRQPKKGAVFAEPQL
                     VVEVEFLMWTRTNTLRAPSFKGLRDDKDPRDVVIEKPDVA"
     gene            7283..7855
                     /locus_tag="VF752_15640"
     CDS             7283..7855
                     /locus_tag="VF752_15640"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX8857028.1"
                     /translation="MSPTPAETTPSEQPVHTIGHSTHEFDEFVGLLRGHGIELLVDVR
                     RYPKSRRVPQFNDDSLAQTLPAAGIEYRHAEALGGRRHPRPDSPNDAWENAGFRGYAD
                     HMASEEFRHALAHLERLADEKPTAVMCAEARWTSCHRRLIADALVARGRPVLHIDSRG
                     RTEPHTLTRFAVVGPGGALSYPASQTSLEL"
     gene            7932..8105
                     /locus_tag="VF752_15645"
     CDS             7932..8105
                     /locus_tag="VF752_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857029.1"
                     /translation="MIGILIAILVAAVVYWICVAIGLPAIVAIIAAILVLLAGIPTGG
                     YGFGSRWGPRRGY"
     gene            8169..9932
                     /locus_tag="VF752_15650"
                     /pseudo
     CDS             8169..9932
                     /locus_tag="VF752_15650"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(9989..10573)
                     /locus_tag="VF752_15655"
     CDS             complement(9989..10573)
                     /locus_tag="VF752_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8857030.1"
                     /translation="MARAGEEITSPFGPRLVFRETSADTNGDAVVVDAYYPPGSSEPV
                     AHYHPKQEEQFEVVSGKITTRINRKLATYGPGDKFVVPKGAPHAMWNAGDEETHVVWA
                     TKPALKTERFFETMWGLARDGRTNSRGVPPLLHVAVIGREYWREFRLTRPAPPVQWVL
                     FRLLAPFGKRKGYRGSYPEYSGELPAPVAPPQQQ"
     gene            10672..11136
                     /locus_tag="VF752_15660"
     CDS             10672..11136
                     /locus_tag="VF752_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857031.1"
                     /translation="MRKILIPAAVIAAAVAVPAQAKSPNPHPPQSHKCKPHSHAYVGS
                     GALVSQSLTQTQGAATATKKDDRWSGDITVNVTRANHHAQKGEQTYTLTDARVRWYDA
                     NHDGTSDVPAAGDRVKLIGKITTLSKKCDQTGFTPTTTIKKAYFKKAKAPKS"
     gene            complement(11191..11865)
                     /locus_tag="VF752_15665"
     CDS             complement(11191..11865)
                     /locus_tag="VF752_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857032.1"
                     /translation="MIKPKIVAVGAGLAVVAGAGGAYAAISDKDKERQAFLNDAASRL
                     NVSPEKLSDALKGAFFDRLDAAVKAGKLTQAQADEIKKGVNQNGGIPFGPGGGPREHH
                     VFGLGPGPGLFGAGFDAATSYLGITQAQLKQQFADGKSLADIAKAKGKSVDGLKTAIQ
                     DAVKAKLDAAVKNKRITSAQESKILSELKSRIDDIVSNSPLRLGHKLERPHGPPPGGP
                     PPPWGP"
     gene            12054..12401
                     /locus_tag="VF752_15670"
     CDS             12054..12401
                     /locus_tag="VF752_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857033.1"
                     /translation="MGKDDLARELDFEGVLAELLGRVGSRVCIDVGDCDERGPPLVMC
                     VVGTLAGGTTVSSYSGHGEAIFFHLGEQPESGVFLNPAHFKCAAIELNGDLSIDLGGV
                     RVVVAPAPQRVGT"
     gene            12426..>12645
                     /locus_tag="VF752_15675"
     CDS             12426..>12645
                     /locus_tag="VF752_15675"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8857034.1"
                     /translation="MPVRAAGIEQFGDAVETLELAEPRALHDGEVLIEVRAAGVANWD
                     DIVRVGDWDVGRRPPMALGVEAAGVVAAV"
BASE COUNT         1924 a         4295 c         4353 g         2073 t
ORIGIN      
        1 cggccttcag ggacgccggc cggcggctgg ccggcgcgcg cgacgcgaag gtcctgatcg
       61 agacgctcga caccgtcgag gaagcagccg gtgacgagct gcgttccggg tcgaccgccg
      121 gcctgcgggt cgagctcgag tcggagcatg aggccgcgct cgaggcgctg cgcgacggcg
      181 gcgccacagc ggcggtacaa agcgacctcg agcacgcgcg cgcccgcgtg gccacttgga
      241 cgctcgccat ggacggcttc gacgcgatcg agcccgggct acggcggatc taccggcgcg
      301 gtcgcaacgc actggaggcc gtcgcggagg agcagagcac cgagaaccgg cacgagtggc
      361 gcacgcgcgt gaaggacctg tggcacgcca ctcagatcgt ccggcccgcc tcgccgagca
      421 gcatgaagaa gctgtcgaga cgcctgcacg acctgtccga cctgctcggc gacgaccacg
      481 accttgccgt cctctgcaca tacgtggcgc ggcacccgcg gtgcttcgaa gaccgggagg
      541 agcaggccgc gctcgtggct gtgatcgagc gcaggcgcga gctcctgcag ggcaaggcgc
      601 tgagcctcgg gcgcactctc tacgagcagt cgcccaagga cttcacgcgc tcgctctcgc
      661 gaccgtggca aaagcgcatg ggcaagcctc gccgaccggc cgcggccgcc tagcgcgcga
      721 ggcctgcagc ttggcgccac cgcccacagc ctggcgggag cgctcgagcg ccctacggcg
      781 ccatcggcag cgccatgcca ggcacgccgc cgttgcccgc gcccggcttg gccgtcggca
      841 gcgggaccac cggcacgctc tgaggcgtgg cggcgatgat gccccgcgcc gcgtgcagga
      901 tcgcctgcga cgcgggcgcg ctcgggtcgt cgatcacgat cggcatgccg gtgtcggcgc
      961 ctacgcgcag ctcctcctgg agcggcacct tgccgagcag cggcacgtcg aggtcgtctg
     1021 cgagcagctg gccgccgcct tcgccgaaga tcgtgaagcg ctcgcccgtc ggtgtcgtga
     1081 agccggacat gttctcgatc acgccgagga tctcgaggtc gaagcggagc gccgtctgcg
     1141 ccgcgcgctg agccaccttc tgcgcggcgg gctgcggtgt ggtcacgatc acgaagcgcg
     1201 cctgcggtag cagctgcgag agcgtcatcg tcacgtcccc ggtgccgggc ggcaggtcga
     1261 tcagcaggta gtcgagctcg ccccagtcga cgtcctcgag gaactggcga atcgccttgt
     1321 gcagcatcgg gccgcgccac gtaacggcct ggtcggcgtc ctcgaggaag tactggatcg
     1381 acatcgtctt gatgcccccg tggctctcga gcgggatgat cttgcgctcg gccgagacct
     1441 tgggccgccc gctcacaccc atcatgcgcg ggatcgagta gccccagacg tcggcgtcga
     1501 gcgccgcggc cgtcttgccc tcggcgaaga gcgccgccgt gaggttggcg gtcatcgacg
     1561 acttgccgac accgcccttg cccgacgcga cgcagatgat gttgttcacc tgcgcgagcg
     1621 cgccggtcgg cagcgtgctg cgctggacgc gctgctggag cccctgcttc tccttagcgg
     1681 agagcacgtc gaagtcgacc ctcaccgagc gcacgccctc gagcgccctg acctgctcgg
     1741 tcaccgcctt ctggaagtga gagcggatcg ggcagccagg cgtggtcagc gagaccacca
     1801 ccgagacggt gccgtcgtcg gccgcgtcga tcgagcggac catgccgagc tcgacgatgt
     1861 ccttcttcag ctcggggtcg acgaccgcgc gcagggagtc gcggatctgc tcctgagtgg
     1921 gcatgactcc attctggcaa agggccccga aaaggcaccg acggcgcccg agggcgccgc
     1981 cggctgaggg aggagaggta ctgcactacc ggcggatatg tgtgccggca gtccttaccc
     2041 tggtccgcgc tctcccggtc gcggccaggg gtggaaaacg gtgctacacc aggttcgaga
     2101 cgttcgagac gaggttctcc gcctggctct cgacctgacg gcgattgcgg cgcacgaagc
     2161 gctcgacctt ggtgcggttg cgcttcaggt cgcgctcgag ctggttgcgg gcggtggtgc
     2221 cgcgacgctc gaacttccgg aggttcacct cgacccggcg ctgcagcctc gagatctcgc
     2281 gctgagcggt ctcgcggctg gtgtacggct tcacgttctc gacgatcgag tcgcgcgcgg
     2341 tgagcgcggc gccgaccgga acgagaaccg cacgctcggc ctgaagcgcg acggcctgga
     2401 ggccggtggc ctcagccttg gcacgctgcg tggcagtcga agcagccgac ttggtcgtgc
     2461 ggcgcgcgtt gctgcgagtg ctggacgccg agcggcgagc cgtgctgcgc tggcgggtgg
     2521 ccgcgccctt cttggcggta gcgcggcgct tggtctgagt ctgggtggcc attgcttgtg
     2581 tccttccccc ctacggtcgg gttgctaagg atattagatg taaggacgtt aggagcagtg
     2641 taccaccgaa cacacgtttg taaacccccg cgtgtttcgg ctttgtaaaa aggaaagacg
     2701 gcgcggtgct aggcggcggc gcgcgagccc tgaatccggt ctacccaggc gccggggtcg
     2761 ctcaactcgc ccagatgagg tagagactcg ggcgactccc aggcccggtt cagcagctcg
     2821 atgccgcccc gtgcggccac tccgtcgcag aagcgcttgc ccaggtcgta ctgccgcatc
     2881 ttgaggtcga ggcccagcag cttctcgagc agccgctgtg gtgccgtccg attcgcgcgc
     2941 cggcgctcca tcgcgtctcg caggccggcg tagttctcga ggtgctcgcc gccgacggcg
     3001 tccatcacat gctcggaata gccctcgatc acggacatcg cggcctgcag gcggcgcatg
     3061 aggtcgcgct gctcgctcgt ctgcacgagc gcgacgagtc cgccctctcg gaactggtcg
     3121 acgagctcgg cgatcgcggg caggcgcgga agtgcgcccg caccgccgct gtcgattcgc
     3181 acctccaccg agtgcaggta ctcgcggagc agacccgcca tgtagccgcg cagccagggc
     3241 acgccagaga actcgaacac gtgcgtcatc tcgtggagcg cgatccaggc gaggaaggcg
     3301 tcggcgtcaa cgccgagctc gccgatcgcg tggccgaggt tcggcaccac gtacagcaac
     3361 cgcgccgacg cgtccggctg aagcagcgag acctcgtact ggccgagcac ccgctgcgac
     3421 atgtagcccg cgatgatgcc cacctcggcg gccagcgtgg cgcccgccgc ggcgcgcagc
     3481 gcgccggcga acgggccggt cttctcgagc cgtccggaca ttcgctcggc gaccgggtcg
     3541 aggaagccct ggagcgtctg gatgttgatc tccgaccact gccgccgccc caccggctcg
     3601 gctgccggca cgggctcgac gagcttgagg ccggtgtaat ccgccaccaa gcgctcggca
     3661 ttcgcggaca tgcccgcgaa gtcggcaccc gctgccttcc cgggcggggg ctcggtgccg
     3721 gcggccagcc ttgctacctg gcgcgcgatc gaccaatcga ccatctgact aaccgtaccc
     3781 acgcgagggt atcgggaact tctatgacgc tcgaggcaca cgaagagcag caccgcgcgg
     3841 cggcgcgaaa cggcgacact gcggagcggc gcgacccaga gcccgtggac tacgccgaga
     3901 tcaacgcggt gtacgcgcta ctcgcgatcg ccgtgatcgc ggtgacgcgc gatcgcgtgc
     3961 gcgaggatcc gatccgcggc tacgaactcc cgcagctggg cgccgcaacg ttcgcgctct
     4021 cgaaggtgat cgcgcgcgag aagatcggga cctggctgcg cgagccgttc gtcgaggagg
     4081 cagacggcga ccggcgcccg cgcggtaagc ggctgcggcg agcgatgggc gagctggtga
     4141 catgcacgcg ctgcgtgggt gcgtggagcg cgctcgggct cgtgtggctg cggctctact
     4201 cgcctgacgc cgggcgcgtg gccacagcgg tactggcgac ctcggcggcc aacgactgga
     4261 ttcaggccgg cttcgcctgg ctcaccggca acgcgaacga gacgaaggcc tcgcgctagt
     4321 cggttgactc aacccgcgaa cggggtaggc accgtccatg ccaacgctct tccgatatct
     4381 gatggcccgc cggttcctgg cccgctacct gggcaagcgc gcggtgcggt tcctgcctgg
     4441 cggctgggtc acgttcatcg cctacccgct cgcccgctgg gcgtggcggc ggcggatgcg
     4501 gcgggcgcgg gcgcgctcgc tagccgcgcg cgccggttag cgccggcagt tcctgctcca
     4561 gctcgacgac cgcagcgaag aggtcgccat cgcgctcgac gcgctcgagc gcggcgtctg
     4621 agtcgaacac gagctcgtcg gggtcgcccg atcgctcggt gctctcgacc tcgtcccagc
     4681 tgatcggcgt ggagaccgtg gggcgctcct tcgcacgaag cgagtagacg ttcacggtcg
     4741 tcttgtgctc gtcgttctgg ctccagtcga cgagcacctt gtgcggcctg agttccttct
     4801 tcatcgagga gaccacgaga tccgggtgct gcttctcgag cagctcggcg agcgccttcg
     4861 cgaacggctt cgtctggtcg taggtgacgt cggtgttgag cggcacgtag acctgcatgc
     4921 ccttggagcc ggacgtcttc gggaacgcct cgatcccgag cgagtcgaag acgtcgcgta
     4981 agagcagcgc gaccctcgag cactcgacga tggtcgcggg cggacccggg tcgaggtcga
     5041 aagcaagggt ggtcggctgc tcgatcgggt cgtatttcga gagcgaggtg tgcagctcga
     5101 ggtcggcgag gttcgcgagc cacacgagcg tcgggagctc gttgcagagg cagaagtcga
     5161 tcgtcttgtt gcgcgccttg atcggcgccg tctcgaccca gtccgggcgg tgcttcgggc
     5221 actgcttctc gtagaagaac ttgtcgtcga cgccgttcgg atagcgcttg agggtgagcg
     5281 ggcggtcgcg cagatgcggc agcagcacgg gcgcgatgcg ggtgtagtag tcgatcacct
     5341 ggcccttggt gaatccgacc ttggggtaca gcaccttctc gaggttgctc agcttgagcg
     5401 tgcgcccgtc gacctcgacc tcaacgctgt tgcttttcgc catgcacgaa gtagtacccc
     5461 atgggcctaa accggagccg cgcgtctgcc gatgcatgct ccgtgagcag cgggtcgtgg
     5521 gatgcagttc agcccgcgcg ggcatccaga ttcgcgcgtc tatcggcgac gctgcgatcg
     5581 ctcgcgtggc tcggcattac gctcgtggtg ctcgtcgcgg gcgtgatcca ctggagtgac
     5641 gaccgctccg ctgacttcgc cggtgcccag atggcgtgcg cgatggcggc ggcgttgttc
     5701 gctctcctcg gagcggcata cacggcgcgc gggcgcaggc actcggcagt gttggcgtta
     5761 gtcctctcgg tcgccgcggc gggcggctgg gtggcgctgc tcttcgtcta agcgacgtcg
     5821 ggcttctcga tcaccacgtc gcgcgggtcc ttgtcgtcac gaaggccctt gaacgacggc
     5881 gcgcgcagcg tgttcgtgcg cgtccacatc aggaactcca cctccaccac gagctggggc
     5941 tcggcaaaca cggcgccctt cttcggctgg cgccccgcga acgggctcgt gtcgcgccgg
     6001 agcggctcga gcagcttccc cagcttcacg aggtcggcct ccttgaaacc ggtgccgacg
     6061 ttgccggcat agatgaggcg ctgctcctca ccgcgctcct cggcctcggc gcgcgtcatg
     6121 ttgtagtagc cgacgcagag cgagccgacg cggccggtac ggccgccctc gcctggcatg
     6181 tatccgccca cgacgagctc ctggcgctgc accgccttga tcttccgcca gcacacgctg
     6241 cgccggccgg gctcgtacgg gctgtcgagc ttcttggcga cgatgccctc gaggccctgc
     6301 tccctcgccg catccagcag cgccttgccc tcgccgcggt gccacgaagg tgtctgccag
     6361 tgggagccgc gcagctcgag ctcgtcgagc agcccgcggc gttcctcata gggcaggcgg
     6421 atggtcacgc ggccgtcgag gtagagcacg tcgaagatcg cgtaaacgac cgggatgtcg
     6481 ctcatgcgcc gccgcaccgc gctctcagac gccaggtgca tccggtgctg caggcgcccg
     6541 aagctcggcc ggccctcctc gtcgaacgcg atgacctcgc cgtcgagcac cagctcgcgc
     6601 gcgccgaccg cccggccgag ctcgcgcagc tccgggtact gctgggtgat atcgcgcatg
     6661 ttgcggttct gcagccgcac gcgaccgccc tcgatgtagg cgatcgcgcg cacgccgtcc
     6721 cacttcacct cgtagcccca ctcgttttcc ttgtccggca cgcgctccgc cagcctggcc
     6781 atcatgggct tgatgtcgcg cggcatgggc tcgcggcctt cgtcggccgg cgggtccatg
     6841 cggtggatca tccagttctt gcccttggtc gggaagagca cgtacttccc ctgcaggcgc
     6901 tccccgtgga agacgaccat cacctctttg tcgcgccact tgagcacgtc gtaggtgccc
     6961 tggtcccaga tcttcacctc gccggcgccg tagttgccct cggggatctc gcccttgaag
     7021 tcgatgtact cgatcgggtg gtcctcgacg tgcaccgcga ggtggttcgt cttcgggtct
     7081 ggcgggatgc ccttcgggat cgcccacgac acgagcacgc cgtcacgctc gaggcgcagg
     7141 tcccagtgga ggctgcgagc gtggtgctcg tggaccacga agcggccgcc gggctgcccg
     7201 cccgagtcgc cgttgcctga cggctccggc gtctcctcga agtcgcgctt gcgcttgtac
     7261 tcgcctagct tcttggccac ggttgtcgcc taccccggca gaaactacgc caagcgagca
     7321 gcccgtccac acgatcgggc actcgaccca cgagttcgac gagttcgtcg gcctcctgcg
     7381 cgggcacggg atcgagctgc tcgtggacgt gcggcgctat cccaagtcgc gacgcgtgcc
     7441 gcagttcaac gacgactcgc tcgcgcagac gctgcctgcg gcggggatcg aataccgcca
     7501 cgccgaggcg ctcggcggcc gccgccatcc gcggccggac tcgcccaacg acgcctggga
     7561 gaacgcgggc ttccgcggct acgccgacca catggcgagc gaggagttcc ggcacgcgct
     7621 cgcgcatctc gaacggctag cggacgagaa gccgaccgcc gtcatgtgcg ccgaggcgcg
     7681 ctggacgagc tgtcaccggc ggctgatagc cgacgcgctc gtcgcgcgcg ggcgccccgt
     7741 gctgcacatc gactcgcgcg gcaggaccga gccgcacacg ctcacgcgct tcgcggttgt
     7801 ggggcccggt ggcgcgcttt cgtatccggc atcacagacc tcactggagc tttagatacg
     7861 ccgaatgtgg cgagagtttg ccccttacga cgagcgggca tcctttcggc gaaaggaggg
     7921 aggcaccaaa agtgattggc attctgatcg caatactggt cgcggcagtc gtgtactgga
     7981 tctgcgtggc cattgggctc ccggccatcg tcgccatcat cgcggcgatt ctcgtgctgc
     8041 tcgccggcat accaaccggc ggctacggct tcggctcgcg ctggggcccg cggcgcgggt
     8101 actgacctct gactgaaatg cgctgaacag cgggcggctt cggccgctcg cttttttccg
     8161 gcggccgatc gatactcgcc tcgatggctc agcgcgtgat cgtcctcggt ggtggggtcg
     8221 ccggaatgac agccgcacac gagctcgccg agcgcggctt cgaggtcacc gtctacgaga
     8281 cacgccccga tccgggcggc aaggcgcgga gcatgcccat cgagacgccg gtgcccggtc
     8341 agccgcagcc gctgccgggc gagcacggct tccgcttctt ccccggcttc tacaagcacg
     8401 tgccggacac gatgaagcgg atcccgttcg gcaacaacaa caacggcgtg ttcgacaacc
     8461 tcgtgcgttc cgagcaagtc gagatcgcgc gcgacggcca atcggagttg atcgcgccgg
     8521 cgcacttccc cgacgacgtg aacgagatcg gcgaggcgtt cggcttcttc ttcgagtacg
     8581 ccacccagct cgggatctcg ctcgacgacc aggtccactt cgcgaaccgg ctgctcctgt
     8641 ttctcacgag ctgtgacaag cggcgcatcg aagagtggga gaagcagagc tggtgggagt
     8701 actcgggggc cgccacgcgc gggacgggct accagaagtt cctcgccgac ggcctcacgc
     8761 gctcgctggt ggccgcgcag gcgaaggaga tgtcggcgcg cactggcgcg tcgatcctgc
     8821 tccagctcct cttcgacatg gcgcagcctg gcggccaggt ggaccgcgtg cttcgcgggc
     8881 cgacgagcga cgtctggatc gggccctggt tcgatctgct cgagaagctc ggagtcgact
     8941 accggctcga gtgccaggtg cagtcgatcg agatggaccg cggtcgcgtg aagggcgtga
     9001 agatcgtgaa ccacggccag cccttcgacg actcggcgga ctggtacgtc gccgccgtcc
     9061 cggtcgaggt gatggacctg ctcgcctctc ccgagattcg gctcgccgat cccagcctcg
     9121 gcggtctctc gcgcctgcgc acgcgctgga tgaacgggat catgttctac ctcgaccgcg
     9181 acgtgccgct ggtccgcggc cacacgatct acatcgactc ggcctgggcg ctgacgtcaa
     9241 tctcgcaggc gcagttctgg aagaacgtcg acctcgagaa gttcggcgac ggcaaggtga
     9301 acggcatcct ctcggtcgac atctcggact ggtacacgcc cgggaccgag aaggtgaaga
     9361 agcaggcgat gtactgctcg cccgagcaag tggccaagga ggtgtgggcc cagctaaagg
     9421 cgagcatcga caccgagggg caggagctgc tcgaggacgc gaacgtggtg cgctggttcc
     9481 ttgacccggc gatcgtctac ccgaacccgg tcgaggcgat gaacctcgag ccgctgctca
     9541 tcaacaccgc cggttcgtgg cccgaccgcc cgcaggcgac gacgaagatc gagaacctct
     9601 tcctcgcctc cgactacgtg cagacctata ccgacctcgc gacgatggag ggcgccaacg
     9661 aggcggcgcg gcgggcggtg aacggcatcc tcgacgcgag cggctcgagc gagaagcgct
     9721 gcaagctctg gaagctgcac gagcccgaca tcttcgagcc gttgaaggcg atcgaccgca
     9781 tgatgttcga gctgcacctg ccgccggtca acctgatcgc gctcgacgcg gcaggcgcgc
     9841 tgcggttcca tcacgtggtg aatgccgtgt ccgacgcgga gcgagtcggc gaaggcgtgg
     9901 cgcgcaggtt gatgggactg gtcggtcttt gaaccgctcg cgggcggggc gggcgctgcg
     9961 cgcgctcgcc gaagatcagg cgctcacgtc actgctgctg tggcggagcc acgggcgctg
    10021 gtagctcacc cgagtactcc ggatacgagc cgcggtagcc cttgcgcttg ccgaatgggg
    10081 ccagcaggcg gaacagcacc cactgaaccg gcggcgccgg gcgcgtgagg cggaactctc
    10141 gccagtactc gcggccgatc acggccacgt gcaggagcgg cggcacgccg cggctgttcg
    10201 tgcgaccgtc gcgcgctagg ccccacatcg tctcgaagaa gcgttcggtc ttgagcgcgg
    10261 gcttcgtcgc ccagacgacg tgcgtctcct cgtcgccggc gttccacatc gcgtgcggcg
    10321 cgcccttcgg gacgacgaac ttgtcgcccg gcccgtacgt cgcgagcttg cggttgatgc
    10381 gtgtggtgat cttgccgctc accacctcga actgctcctc ctgcttgggg tggtagtggg
    10441 cgacgggctc cgagctgccc ggtgggtaat aggcgtcgac cacgacggcg tcgccgttcg
    10501 tgtccgcgct cgtctcgcga aagacgagcc gcggtccgaa ggggctcgtg atctcctctc
    10561 ctgcccgggc catggacggc ggagtctatg cagggcagaa gtagcgcctg atttacctgg
    10621 cgcgcgcacg ttactgcctc caagcacgtg aaacacggag gtacctcaag catgcgaaag
    10681 atcctgatcc cggccgcggt gatcgcggcc gccgtggcgg tcccggcgca ggcgaagtcg
    10741 cctaacccgc acccgccgca gtcgcacaag tgcaagcccc atagccacgc ctacgtgggc
    10801 tcgggcgcgc tcgtgagcca gtcgctcacg cagacccagg gcgctgcgac cgcgacgaag
    10861 aaggacgacc gctggagcgg cgacatcacc gtgaacgtga cgcgagcgaa ccaccacgcg
    10921 cagaagggcg agcagacgta cacgctcacc gacgcgcgcg tgcgctggta cgacgcgaat
    10981 cacgacggca cgtcggacgt gccggcagcc ggcgatcgcg tgaagctgat cggcaagatc
    11041 acgacgctct cgaagaagtg cgatcagacc ggcttcacgc ccaccaccac gatcaagaag
    11101 gcctacttca agaaggcgaa ggcgccgaag tcgtaagcgg cttctccgat gcacaaggaa
    11161 agggccgccc tcgggcggcc ctttctcgtc ctacggtccc cacgggggcg gcgggccgcc
    11221 cggcggaggg ccatgcggcc gctccagctt gtgccccaac ctgagcggtg agttgctgac
    11281 gatgtcgtcg atccggctct tcagctcgct caggatcttc gactcctgag cggaggtgat
    11341 ccgcttgttc ttcaccgcgg cgtcgagctt ggccttgacg gcgtcctgga tcgcggtctt
    11401 gaggccgtcg accgacttcc ccttggcctt cgcgatgtcg gcgagcgact tgccgtccgc
    11461 gaactgctgc ttcagctggg cctgagtgat gccgaggtag ctcgtcgccg cgtcgaagcc
    11521 ggcgccgaac aaccctgggc ctggaccgag gccgaagaca tggtgctcac ggggaccgcc
    11581 tccgggaccg aacgggatgc cgccgttctg gttcacgccc tttttgatct cgtcggcctg
    11641 ggcctgggtg agcttcccag ccttgacggc cgcgtcgagg cggtcgaaga aggcgccttt
    11701 gagcgcgtcg ctcagcttct cgggtgagac gttcagtcgg ctggccgcat cgttcaggaa
    11761 cgcctgccgt tccttgtcct tgtcgctgat ggccgcatag gcgccccccg cgcctgccac
    11821 caccgcgagc ccagctccta cggcgacgat cttgggtttg atcaatgctc cacggctcct
    11881 tccggggtcg ggctgactct tggaatgcca ccagccggcg ataagaggcc gcttaactca
    11941 ggcgaagaag cgcgtaagag ctcgcgcgcc aaggtcacat cggcagggac atttgaccaa
    12001 acaaactcgc tcgctcagcg ttatgtatcg aacgagggcg atccgacggg gcgatgggga
    12061 aagacgatct cgcacgcgag ctcgacttcg aaggtgtgtt agccgagttg ctcggccgcg
    12121 tgggctcccg cgtgtgcatc gacgtcggcg actgcgacga gcgcgggcct ccccttgtga
    12181 tgtgcgtcgt cggcacgctc gccggcggta cgaccgtcag ctcgtacagc gggcacggag
    12241 aggccatctt cttccacctc ggagagcagc ccgagtcggg cgtcttcctg aaccccgcgc
    12301 acttcaagtg cgcggccatc gaattgaacg gcgacctctc gatcgacctc ggcggcgtcc
    12361 gcgtcgtcgt cgcgcccgcc ccacagcggg tcggcacctg aaacgggcgt cgtcgttcac
    12421 aattgatgcc ggtgcgagca gccgggatcg aacagtttgg cgacgccgtc gagaccctcg
    12481 agctcgccga accgcgcgcg ttgcacgacg gtgaggttct catcgaggtc cgggcggcgg
    12541 gtgtcgcaaa ctgggacgac atcgtgcgtg tcggcgattg ggacgtcgga cgccggccgc
    12601 cgatggcgct tggcgttgag gcggcgggcg tcgtggctgc cgtcg
//
LOCUS       DASVXS010000278         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_762198, whole genome shotgun sequence.
ACCESSION   DASVXS010000278 DASVXS010000000
VERSION     DASVXS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762198"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="rpsE"
                     /locus_tag="VF752_15680"
     CDS             complement(<1..297)
                     /gene="rpsE"
                     /locus_tag="VF752_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX8857035.1"
                     /translation="MPVPNRTVDSSGYDLQERVVEINRVAKVVKGGRRFSFTALVVVG
                     DERSVVGVGYGKANEVPLAIQKGVERAKKNLFRVPMHGSTITHQSTGVYGAGRVL"
     gene            complement(334..687)
                     /gene="rplR"
                     /locus_tag="VF752_15685"
     CDS             complement(334..687)
                     /gene="rplR"
                     /locus_tag="VF752_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX8857036.1"
                     /translation="MSVQTRPAKRLRRRRRVRAKVAGTTERPRLSVFRSNRGIQAQVI
                     DDTKGQTVAAVNWIEKDLRGLPSMDQAKKAGELVAQRAKDAGVESVVFDRGGYRYHGR
                     VKALAEGAREGGLAF"
     gene            complement(684..1217)
                     /gene="rplF"
                     /locus_tag="VF752_15690"
     CDS             complement(684..1217)
                     /gene="rplF"
                     /locus_tag="VF752_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX8857037.1"
                     /translation="MSRIGKQPIPVPDGVSVSIEPEVVRVNGPKGNLEQRVPREIAVE
                     QQDGEIVVTRPTDRGEHRSLHGLTRSLIANMVQGVTEGFQKNLQIQGVGYRAQLQGNK
                     IVLAVGYSHPVEINAPAGIEFEVPQPTQIIVRGIDKQAVGEVAARIRKVRPPEPYKGK
                     GIRYQDEYVARKVGKRA"
     gene            complement(1225..1623)
                     /gene="rpsH"
                     /locus_tag="VF752_15695"
     CDS             complement(1225..1623)
                     /gene="rpsH"
                     /locus_tag="VF752_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX8857038.1"
                     /translation="MSMTDPIADFLTRIRNGLAADHDSVDIPASTFKTQIARILQEQG
                     YIEGFSTEAARVGEVLRVRLRYTEDRQPVISGIQRRSRPGRREYVGALEIPKVLGGMG
                     TTIVSTSKGVMTGHEARRQGVGGEIVAEVW"
     gene            complement(1628..1813)
                     /locus_tag="VF752_15700"
     CDS             complement(1628..1813)
                     /locus_tag="VF752_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX8857039.1"
                     /translation="MAKKSQVVRASRPQKFKTREYHRCRRCGRPRAYFRKFGLCRICL
                     REVAHEGYVPGMTKSSW"
     gene            complement(2042..2596)
                     /gene="rplE"
                     /locus_tag="VF752_15705"
                     /pseudo
     CDS             complement(2042..2596)
                     /gene="rplE"
                     /locus_tag="VF752_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(2596..2922)
                     /gene="rplX"
                     /locus_tag="VF752_15710"
     CDS             complement(2596..2922)
                     /gene="rplX"
                     /locus_tag="VF752_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX8857040.1"
                     /translation="MKIRRGDTVKVISGKAKGRTGRVLRVEPDKERVYVEGLNMIKRH
                     QRPRTLRDTQRGGEVGGVIEKEGPIHVSNVMLLDPKTNEPTRVRIERRDGRRVRVAAR
                     SGEEID"
     gene            complement(2937..3305)
                     /gene="rplN"
                     /locus_tag="VF752_15715"
     CDS             complement(2937..3305)
                     /gene="rplN"
                     /locus_tag="VF752_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX8857041.1"
                     /translation="MIQPETRLRVADNTGAREILCIQVQGGSKRRYAGVGDTIVATVK
                     QAIPQGSVKKGDVVRAVVVRTKKEYGREDGTYIAFDENAAVLIDAQQNPRGTRIFGPV
                     ARELRDRNFMKIVSLAPEVL"
     gene            complement(3302..3574)
                     /gene="rpsQ"
                     /locus_tag="VF752_15720"
     CDS             complement(3302..3574)
                     /gene="rpsQ"
                     /locus_tag="VF752_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX8857042.1"
                     /translation="MPAVETEGTGRKRVRQGVVVSAKPDKTITVRIDTAHRHRTYKKI
                     VRSSTTLRAHDERNEANEGDMVRVIESRPISRSKRWRLVEILERAR"
     gene            complement(3937..4158)
                     /gene="rpmC"
                     /locus_tag="VF752_15725"
     CDS             complement(3937..4158)
                     /gene="rpmC"
                     /locus_tag="VF752_15725"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX8857043.1"
                     /translation="MKRTDVNGLADDQLIEFVANARQDLFNLRFQHATGQLENTAALP
                     RAKRELARGLTVARARGIDVDRELKRQEA"
     gene            complement(4155..4574)
                     /gene="rplP"
                     /locus_tag="VF752_15730"
     CDS             complement(4155..4574)
                     /gene="rplP"
                     /locus_tag="VF752_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX8857044.1"
                     /translation="MLTPKRVKHRKQHRGRRAGYAKGGTRVEFCEYGLKTLERGWITN
                     RQIEAARIAMTRRIKRGGKVWINIFPDKPVTAKPAETRMGSGKGSPEGWVAVVKPGRI
                     MFELAGVPEPLAREAMRLAGHKLPVKTKFVVREGAEQ"
BASE COUNT          568 a         1674 c         1538 g          855 t
ORIGIN      
        1 caatacgcgg cccgcgccgt agacgccggt gctctggtgc gtgatcgtcg agccgtgcat
       61 cggcacgcgg aagaggttct tcttcgcgcg ctcgacgccc ttctggatcg cgagcgggac
      121 ctcgttggcc ttgccgtagc cgacgccgac caccgagcgc tcgtcgccca ccaccacgag
      181 cgcggtgaac gagaagcggc ggccgccctt gaccaccttg gccacgcggt ttatctccac
      241 gacccgctcc tgcaggtcgt agccgctcga atcaactgtc ctgttaggta ctggcatccg
      301 tccgtccgag ggtatcgaag tcttatttgg cggctagaaa gccagaccgc cttcgcgcgc
      361 gccctctgcg agggccttca cgcggccgtg gtaccggtac ccgccgcggt cgaagacgac
      421 ggactcgacg cccgcgtcct ttgcccgctg ggcgacgagc tcgccggcct tcttggcctg
      481 gtccatcgag ggcagcccgc gcaggtcctt ctcgatccag ttcaccgcag caaccgtctg
      541 gcccttcgtg tcgtcgatca cctgggcctg gatcccgcga ttcgaccgga acacagacag
      601 ccgcggccgc tcggtcgtgc cggccacctt cgcgcgcact cgccggcggc ggcgcagtct
      661 ctttgccggc ctcgtctgga cgctcatgcg cgcttaccga ccttccgggc cacgtactcg
      721 tcctggtagc ggatgccctt gcccttgtag ggctcgggcg gccgcacctt gcggatgcgg
      781 gcggcgacct cgccgacggc ctgcttgtcg atgccgcgca cgattatctg cgtgggctgc
      841 ggtacctcga actcgatgcc ggccggcgcg ttgatctcga ccggatgcga atagccgacc
      901 gcgagcacga tcttgttgcc ctgaagctga gcgcggtagc cgacgccctg gatctgaagg
      961 ttcttctgga agccctcggt gacgccctgg accatgttcg cgatcaggct gcgggtgagg
     1021 ccgtggagcg agcggtgctc gccgcggtcg gtggggcgcg ttacgacgat ctcgccgtcc
     1081 tgctgctcca ccgcgatctc gcgcggcacg cgctgctcga ggttgccctt cgggccgttc
     1141 acgcgcacga cctcgggctc gatcgagacc gaaacgccgt cgggcacggg aatgggctgc
     1201 tttccgatcc ggctcaccga gcgctcacca cacctcggcc acgatctcgc cgcccactcc
     1261 ctggcggcgc gcctcgtgac ccgtcatcac gcccttcgac gtggagacga tcgtcgtgcc
     1321 catgccgccg agcacctttg ggatctcgag tgcgccgacg tactcacggc ggcccgggcg
     1381 cgagcgccgc tggatgcccg agatcaccgg ctggcggtcc tcggtgtagc gcagccgcac
     1441 gcgcagcacc tcgccgaccc gcgcggcctc ggtcgagaag ccctcgatgt agccctgctc
     1501 ctggaggatc cgggcgatct gcgtcttgaa cgtgctcgcg gggatgtcca cggagtcgtg
     1561 gtccgccgcg agcccgttgc ggatgcgcgt caggaagtcg gctatgggat cggtcatgga
     1621 catacggcta ccagctggac ttcgtcattc cggggacgta cccctcgtgg gccacctcgc
     1681 gcaggcagat ccggcagagg ccgaacttgc ggaagtacgc gcgggggcgg ccgcagcgcc
     1741 tgcagcgatg gtattccctg gtcttgaact tctgcgggcg cgatgcgcgc accacctgtg
     1801 acttctttgc cattggctcc ttctaacttt cttcgccgcc gccctcggtg ggctcggctt
     1861 ctgcagcttc ctcgtcgccc gtgggctcgg gcttggcgta ggcctccggg ttctcctcct
     1921 tgagagcctc gagcgcggcc tgctccgcct cggccttggc ccgcgcctcc tccctgcgct
     1981 tctcctcctc ggcggcctcg cgcgcctcga cgtcatcgcc gcccggccgg ccctcgcgcg
     2041 agaacggcat gccgagcgag tcgagcagcg cgaatgcctc ctcgtcggta ttcgccgtcg
     2101 tggtgatcac gacgtcgagc ccgcgaacct gatcgatcgc gtcgtagtcg atctcgggga
     2161 agatgatctg ctcccgcacg ccgaccgtgt agttgccgcg cccgtcgaac gagcgcaggc
     2221 tgagcccgcg gaagtcgcgg atgcgcggga tcgcgaccga cacgaagcgg tccaggaact
     2281 cgtacatgcg cgcgtcgcgc agcgtcacgg ccacgccgac cggattgccg tcgcgcagct
     2341 tgaatgcggc gatcgacttg cgcgcccggc ggacattcgg cttctgcccg gcgatcgtgg
     2401 cgagctgctc ctgcgcggac tcgagcacct tcgtgtcctg cttggcgtcg cccacgccca
     2461 tgttcagcac gatcttctca agccgcggcg cctgcatcgg cgacgagtag ccgaagcggc
     2521 tgatcaaggc aggctggatc tcctcgaagt actgccgctt caggcgcggc acgacctttg
     2581 cttccgcaac tgccatcagt caatctcctc cccggagcga gcggccacgc ggacacggcg
     2641 gccgtcgcgg cgctcgatgc gcacgcgggt gggctcgttc gtcttcgggt cgagcagcat
     2701 cacgttgctc acgtggatcg ggccttcctt ttcgatcacg ccgcccacct cgccaccgcg
     2761 ctgcgtgtcg cgcagcgtgc gcggccgctg gtggcgcttg atcatgttga ggccctccac
     2821 gtacacgcgc tccttgtcgg gctccacgcg caggacgcgg cccgtgcggc ccttggcctt
     2881 gccggagatg accttgaccg tgtcgccacg gcggatcttc agtgcgtgcg gcatcgctac
     2941 agcacctccg gggcgaggga gacgatcttc atgaagttgc gatcgcgcag ctcgcgggcg
     3001 accggcccga agatgcgcgt gccgcgcggg ttctgctggg cgtcgatgag caccgctgcg
     3061 ttctcgtcga acgcgatgta ggtgccgtcc tcgcggccgt actccttctt cgtgcgcacg
     3121 accactgcgc gaacgacgtc gcccttcttg acggacccct gtgggatcgc ctgcttcacg
     3181 gtcgcgacga tcgtgtcgcc cacgcccgcg tagcggcgct tcgaaccgcc ctgcacctgg
     3241 atgcagagga tctcgcgcgc gccggtgttg tcggccaccc tgagtcgcgt ctccggctgg
     3301 atcatcgggc gcgctccagg atctccacga ggcgccagcg cttgctgcgg ctgatcggac
     3361 ggctctcgat cacgcggacc atgtcgccct cgttcgcctc attgcgctcg tcgtgcgcgc
     3421 gaagcgtggt cgacgaacgg acgatcttct tgtaggtgcg gtggcggtga gccgtgtcga
     3481 tgcgcacggt gatcgtcttg tccggctttg cggacacgac cacgccctgg cgcacgcgct
     3541 tgcgacccgt gccctccgtt tcgacggcgg ggatggcggc gggctgagtc gcctcgggac
     3601 gagccgcgcg cttctcgcgc tgcttcgccc tccaggcgcg ccgctccctc gcgttcgtct
     3661 gacggcgggc ggcgcgctcg gcggtccgct cctctgggcc gcgctgcggg cgcgcctcgc
     3721 ctgcgcgctg cgagcgcgca cggcggcggc gctccttcgg atggagcgcc tcctcgggct
     3781 cagcttcggt tgtctcggcg gctgcagcct ccggggcgtc gtcgccctcg gcggcggggg
     3841 cgtcctcggc agcctcgtcg gccgcctcct cgaccggagc ctcggcctcg gccgtcggct
     3901 cctcggcctc cgcctgcggg ttctcctgct cttcgatcat gcttcctgtc gcttcagctc
     3961 tctgtctacg tcgattccgc gggcacgcgc gaccgtgagg ccgcgggcga gctcgcgctt
     4021 ggcgcgcggc agcgcggcag tgttctccag ctggccggtg gcatgctgga aacgaaggtt
     4081 gaagaggtcc tggcgcgcgt tcgccacgaa ctcgatcagc tggtcgtcgg ccagaccgtt
     4141 cacatcggtg cgcttcactg ctcagcgccc tcccgcacga cgaacttcgt cttcacgggc
     4201 agcttgtgcc ccgcgaggcg catcgcctcg cgcgccagcg gctcgggcac gccggcgagc
     4261 tcgaacatga tgcggccggg cttcaccacg gccacccagc cctcggggga gcccttgcct
     4321 gagcccatgc gggtctcggc cggcttcgcc gtgacgggct tgtccgggaa gatgttgatc
     4381 cagaccttgc cacctcgctt gatccggcgg gtcatcgcga ttcgggcagc ctcgatctgg
     4441 cggttggtga tccaaccgcg ctcgagcgtc ttcagcccgt actcgcagaa ctcgacgcgg
     4501 gtgccgccct tcgcgtagcc ggcacgccgg ccgcggtgct gcttgcggtg ctttacgcgc
     4561 ttgggagtga gcatgggcta gccgttgccc ctctggcctg aaggattgcc gccgccctgc
     4621 cgcgggccgc cgcca
//
LOCUS       DASVXS010000279        12644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_836695, whole genome shotgun sequence.
ACCESSION   DASVXS010000279 DASVXS010000000
VERSION     DASVXS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12644
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836695"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VF752_15735"
     CDS             complement(<1..646)
                     /locus_tag="VF752_15735"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857045.1"
                     /translation="MRCDVHAIAPGVRKPEAYAGSAGEEAAERVRQLAEPLAGARVLH
                     LASAAAPARPPEPLPGLVALLRGSGLDAGWRLLGGDREWFELARQLEDGLRGGETALE
                     PPALEAFRMAVGEAAGALIEESDVVVLHGAAALGAAPDAAVPLVWAPDAPGAAVGEQA
                     WDVLAPAAGRCRAWLLTGGASAPRAADGVKLHGANAALDPLSAAALDLPVATAGA"
     gene            877..1350
                     /locus_tag="VF752_15740"
     CDS             877..1350
                     /locus_tag="VF752_15740"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX8857046.1"
                     /translation="MRKPLLSVLGVGLAVAPVTVALAAEPHTDTTKAQDTTTVSAADK
                     RVSMQVPREARDKQRLAHRRAAHKRHRASAKRAAAAAPATPPALQAIAACESGGNPAA
                     VGGGGRFRGKYQFDYGTWASVGGSGDPAAASEAEQDKRAAILYSRAGSSPWPVCG"
     gene            complement(1362..2714)
                     /locus_tag="VF752_15745"
     CDS             complement(1362..2714)
                     /locus_tag="VF752_15745"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX8857047.1"
                     /translation="MLTALACAALVGGVAKPASADLPGRLPGSLPIIPGITPGTTYRE
                     QPPPSDEGMLPFASDSFDTVYPGALHHFFYAIGSNELVDANDAQLAATARRNALIVLP
                     PWKDQIAAKLKAANPGLRVLVYKETFVSTESDKATGKSVSGVPWAKAKPSWLLRGGDG
                     QPLASSSVPYYYFADVGNPAYQDAWASNVLREITARGSPWDGVFIDDFLPTTYYHADG
                     NVGEVAKYPSDQQWQPAMASFAARVGPRVQAAGKLAIANMGGTWADPRYAEWNTSVVL
                     SYLSGGLDEFFTHWWGSDEPQDSSYFDTVQRIGQRLEQAGKIFLAQTTNNQEPDARAS
                     LYGYVTALMFANGHTAFSYASDPSYDVGESWLPIYGMPVGRALGAAAPLYDQSQPGPP
                     VWIRKFSRALAIANPGDSPKTVAFDVREYCNVPPGALVAGHTTVGPRAGALLDPTVFC
                     "
     gene            complement(2760..4076)
                     /locus_tag="VF752_15750"
     CDS             complement(2760..4076)
                     /locus_tag="VF752_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8857048.1"
                     /translation="MSAGSARAVDREILRLALPALGALAAEPLYVLADTAIVGHLGTD
                     QLAALAIAATLITGSFTIFNFLTYGTTAQVARYHGAGEEEIAGRLAAQALWLALGIGA
                     LLAALAVGLARPAVSLMGGDGRVADLAVLYLRISAIGLPCAMVALAGQGYLRGVSDFR
                     LPLVIVIAANVANVVLEVVFVYVFDWGIAGSAWGTVIAQLAMASAFAAALLRAPADTR
                     RPNLAYLRPLLRVGGDIAVRTTALYASFLLASALAARVGSASLGAHQIGFQLFVFLAL
                     VLDAIAIAGQVIVGRTLGAGGADEAYASARRMIGWSVVVGLAFAVVLGSLVAVIPHAF
                     TDDPRVIDRAHVMWPFLAIMQPAAGAVFALDGILIGAGDTRFIKWAMVAAFACFAMVA
                     LITYQLDLGIAGVWTALIALIAVRLVTGLARFQGRRWLVTGAPATA"
     gene            complement(4063..4806)
                     /locus_tag="VF752_15755"
     CDS             complement(4063..4806)
                     /locus_tag="VF752_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8857049.1"
                     /translation="MIVERSMQPDWLSNSYLVADEEGGAGVVIDSGGPAEPIIEAIDR
                     HGLTVRHLLLTHHHHDHVAENKAYRDRYGVEILAHPLEAEFLADVDRTIEPGETLEVG
                     GLSIDSIHTPGHTAGMLNFFVNGSDVFTGDTLFKGSVGGVRAPGSTSFEDIRSSIMDR
                     LMKLPHEVTVHPGHTDPTTIGDEWEHNRFIRIWRGEDPEGSDRCSVDGEGATLVLWAP
                     DYDGGHKAWVRWDESGKDDIVPGSRVERG"
     gene            4833..5927
                     /locus_tag="VF752_15760"
     CDS             4833..5927
                     /locus_tag="VF752_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8857050.1"
                     /translation="MPAADAAAFRAEFPVLERIAYLNTGSVGPVPSRGAAAARERIDT
                     ELLEGRIGQSAWEGLLALSERLREAYARVLGGDIDEVAVTRSTTDGCNAVIWALELGE
                     GDEVLTSDEEHPGLLAPLNQARRRRGISVRVVPFAELASAITPQTKLVACSHVSWLSG
                     QVADVEALAAADPMVLLDGAQGLGAVPVDVPALGCDFYAASGQKWLCGPNSSGCLWVR
                     SERLAEMSTPWPIFESVVDPEHALDSPMREDAHRFDSGVLASSSAAWSLAAIELFEEY
                     GLAWMQERSGSLAEWLANTLRERGLDVMPRGRSTLVSWRDPDPPTTVERLAAQGLVLR
                     HIPGAGVVRASVGAWSSEQELDRLVDAVGR"
     gene            complement(5924..6376)
                     /locus_tag="VF752_15765"
     CDS             complement(5924..6376)
                     /locus_tag="VF752_15765"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8857051.1"
                     /translation="MGEGFEGYRSFAPEGWDPPADVNDFMAITERLSYPEVWAMLAER
                     EGEVAGHVAFAPARSTRWGSPEAGLAHLWQLFVREPYWGTGIATALHFEAMAEAARRG
                     FTSIRLYTPSGQARARRFYEREGWTTLGEPFVQEELGLELVEYRRPLS"
     gene            complement(6439..6681)
                     /locus_tag="VF752_15770"
     CDS             complement(6439..6681)
                     /locus_tag="VF752_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857052.1"
                     /translation="MIATVVEWKTLGESVLYSLVGGIGVTTAFSIAIAGATRSYELAR
                     DGRRSHATAYGVLAGVAVLVCLAAISVGIYYTTKKT"
     gene            complement(6678..7784)
                     /locus_tag="VF752_15775"
     CDS             complement(6678..7784)
                     /locus_tag="VF752_15775"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX8857053.1"
                     /translation="MSNDIVLVIVVATALLFDFTNGFHDTANVVATSISTRAMPPRLA
                     VLFAALLNFAGAFISIKVAATVAKGIVDAGAVTPTVVFAGLVGAIAWNLITWWYGLPS
                     SSSHALIGGMIGATLAAAGASAVSADGVFGKVVIPALVAPTVAFLTAAVSILLAYRII
                     GRLRPGPVSNGFRIGQVASGGLLALAHGTNDAQKTMGVITLALIANGNLPEGANPPTW
                     VIVSAAAAIALGTYSGGWRIIRTMGMRIIKMDPAQGFAAQGAGAAVILASSHFGYPLS
                     TTHVISGGVMGAGAAKRVSAVRWGVAGNMVTAWILTLPAAGGIGAAVYGITRLFGTGA
                     TGPIVVAILILVGLTVIFVRRVQRGSPITAEART"
     gene            complement(7791..8417)
                     /locus_tag="VF752_15780"
     CDS             complement(7791..8417)
                     /locus_tag="VF752_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX8857054.1"
                     /translation="MARLSQAFLPRDREFFDLFEEAGGNIARAAALLEQMLRDYPEQA
                     ELAREILICEQHGDRITHDIIQRLNNTFVTPIDREDIHQLASALDDILDFTEETADFL
                     GLYKIEAPMEPAQQLAKVLVQSGRLIAEAMPRLRGFNDISHYTVELNRLENDGDRIHR
                     EALASLFETGTDPMVVIRWKDIYDRLEAAIDATEQVAYILEGIVIKNS"
     gene            complement(8522..9214)
                     /locus_tag="VF752_15785"
     CDS             complement(8522..9214)
                     /locus_tag="VF752_15785"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8857055.1"
                     /translation="MLAVVYDVHGNLPALEAVLEDAEQVGADSFFLGGDYVGMGAWPE
                     ETLARLQQLENATWIRGNWERWQAHPEDAPDDEWLQRAIAYAEGALGGNLVAELAALE
                     QARVYEGALYCHASPQSDMRSFFPEPAEDEDELIDGVAEPTLVFGHTHLQFSRTSEHG
                     ILMLNPGSVGLPFDGDRRAAYALIDENGGIELRRVGYDSDAAAMDTRARMGELGEPTA
                     LRLEAARFDVEA"
     gene            complement(9214..9483)
                     /locus_tag="VF752_15790"
     CDS             complement(9214..9483)
                     /locus_tag="VF752_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857056.1"
                     /translation="MAAKRDARMVALGYGKFVRADRVFALVPLEPAERGDGRRTYVHV
                     DGLSEPIVASRSEKAILEDVEAALVEAAGVPRPRRRGARAGETLF"
     gene            9585..9713
                     /locus_tag="VF752_15795"
     CDS             9585..9713
                     /locus_tag="VF752_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857057.1"
                     /translation="MLSACLLFTALVAIWGSPTPRLLKELFEQSRVEGDQPAEPVL"
     gene            complement(9905..10894)
                     /locus_tag="VF752_15800"
     CDS             complement(9905..10894)
                     /locus_tag="VF752_15800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8857058.1"
                     /translation="MRALQISELTGPDGLSVVDVPEPGASHMLTPGEGVLIDVEAAAV
                     SFPDVLQSRGLYQFKPDLPFVPGAEVAGTVVDVSGNADVKPGDRVAAMTMLGGMAERV
                     VAPAPLTFKLPDQLDLAQGAALILNYHTAYFSLKLRGRLEPGEQVLVHGAAGGVGTAT
                     LQVAKGLGAKTLAVVSSDEKERVAREAGADEVVRSDGPWKDQVVELSGGGVDIVIDPV
                     GGDRFTDSLRSLREDGRLVVVGFTGGSIPEVKVNRLLLKNTEVVGAGWGAYVMSKPEL
                     NRQIGAEIELLVNEGYVNPIVGARFPLARAADAMKLIDSRGATGKVVLDVPGE"
     gene            complement(10950..11022)
                     /locus_tag="VF752_15805"
     tRNA            complement(10950..11022)
                     /locus_tag="VF752_15805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10987..10989),aa:Val,seq:gac)
     gene            complement(11070..12065)
                     /locus_tag="VF752_15810"
     CDS             complement(11070..12065)
                     /locus_tag="VF752_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8857059.1"
                     /translation="MRYLVTGGSGYIGSRLVQRLLERDDTDRIVVADVRPPRTFNPKV
                     EYVQMDVRDKVAVGATLAREKPDVLVHLAFILNPMHDENAMYDIDVGGTQNVLEAAEK
                     AGIEQLLVTSSTTAYGAFPDNPNPIPEDHPVRGVPDFEYARDKAECDRLCQLWALRNP
                     DRVMTIVRPCIVMGPNVDNYIVRLWTDQPFQADFGLPDVDFQFVHEDDLVDAIIGLIV
                     GRHAGAFNVAGDGVIPGAQLAEMIGLPRRKVPLKLFWRFAGLMWKLRQAETPPGNLHF
                     AIHPWVTSNEKVKATLGWEPRYSSLEAFQEAMRARGKLPREGASLSVNVGAPLGA"
     gene            complement(12104..>12644)
                     /locus_tag="VF752_15815"
     CDS             complement(12104..>12644)
                     /locus_tag="VF752_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857060.1"
                     /translation="FAVAAAVLAGCGGGEKPAGSSGGKEVRAVGGDYVYTVPPGFVEE
                     PVRGKLASQSESHTTAVGLTGDSKSGVVVHEVPDSTGARNVDELAAFVAPRFRDVDGK
                     VPEPAHARMAGRPALRFDIQGRSVRSGDRLDGEAWFVLAPRGQLTVVSCQWPPNRRDK
                     VAPGCAWVVKTLRVSERRG"
BASE COUNT         1887 a         4489 c         4401 g         1867 t
ORIGIN      
        1 gcgcgcctgc ggtggcgacg ggcaggtcga gcgctgccgc tgagagcggg tcgagcgccg
       61 cgttcgcccc gtgcagtttc accccgtccg ctgccctcgg cgcggacgca cctccggtca
      121 agagccaggc gcggcagcgg ccggctgccg gcgccagcac gtcccaagcc tgctcgccga
      181 ctgcagcgcc gggtgcgtcg ggcgcccata cgagcggcac ggcggcgtcc ggggcggccc
      241 cgagcgcggc cgcgccgtgc agcaccacca cgtcgctctc ctcgatcagc gccccggccg
      301 cctcgcccac ggccattcgg aacgcctcga gcgcaggggg ctcgagcgcc gtctcccctc
      361 cgcgcaggcc gtcctcgagt tggcgggcga gctcgaacca ctcccggtct ccgccgagca
      421 gccgccaccc cgcgtcgagt cccgacccgc gcagaagcgc gacgagcccg ggcagcggct
      481 cgggcgggcg cgcgggcgcc gcggcggatg cgaggtgaag cacgcgcgcc ccggcgagcg
      541 gctctgccag ctgccggacc cgctccgcgg cctcctcccc cgcactcccc gcgtaggcct
      601 cgggttttct cacaccgggc gcgatcgcgt ggacgtcgca tctcacgacc gaggcagcct
      661 accccggcca gcacgattgc gggaggccgc ttgcaatgtg acgcacgtca cggtggcatc
      721 cctcgaacgg ggtacggtcg aagacgaacc ggctggcagg ggcatggacg ttgcggtcgg
      781 ttccctttcc cgcgcgggag cacatgggat ctgcgcgcga gagcgcgccg gagtgaggag
      841 aggtcggcct gaacccgacc cgagaggtgt tttcccttgc ggaagcccct tttgtcggtg
      901 ctcggcgtcg gcctggccgt cgcgccagtg accgtggcgc ttgccgccga gccgcacacc
      961 gacaccacca aggcgcagga cacgaccacg gtcagcgcag cagacaagag agtgagcatg
     1021 caagtccctc gcgaggcccg ggacaagcag cggcttgccc accggcgcgc ggcacacaag
     1081 cgtcaccgcg cgagcgcaaa gcgtgcggcc gcagcggcac ccgctacgcc cccggctctc
     1141 caggcgatcg ccgcctgcga gtccggcggt aacccggccg ccgtaggcgg cggcggcagg
     1201 ttccgcggca agtaccagtt cgactacggc acctgggcct cggtcggcgg tagcggtgat
     1261 cccgcagccg cctccgaggc cgagcaggac aagcgcgccg cgatcctcta ttcgcgcgcg
     1321 ggctcgtcgc cctggccggt ctgcggctga gccgcttcga gtcagcagaa gacggtcgga
     1381 tcgagcaggg cgccggcccg cggtccgacc gtcgtgtgcc cggcgaccag cgcgccgggt
     1441 ggcacgttgc agtactcgcg gacgtcgaag gcgaccgtct tcgggctgtc gcccgggttc
     1501 gcgatcgcca aggcgcggct gaacttgcgg atccacaccg gcggcccggg ctgcgactgg
     1561 tcgtagagcg gcgcggccgc gccgagcgcc ctgcccacgg gcatcccgta gatcggaagc
     1621 cagctctcgc ccacgtcgta gctcggatcg gacgcgtacg agaaggcggt gtggccgttg
     1681 gcgaacatca gcgcggtcac gtagccgtag agcgaggcgc gtgcgtcggg ctcctgattg
     1741 ttcgtggtct gggcgaggaa gatcttcccc gcctgctcga gcctctggcc gatccgctgc
     1801 acggtgtcga agtacgacga gtcctgaggc tcgtcactcc cccaccagtg cgtgaagaac
     1861 tcgtcgagcc cgcccgagag atatgacagg acgaccgacg tgttccactc ggcgtagcga
     1921 gggtcggccc aggtcccgcc catgttggcg atcgcgagct tgcccgcggc ctgcacacgc
     1981 gggccgacgc gcgcggcgaa cgacgccatc gccggctgcc actgctggtc gctcgggtat
     2041 ttcgcgacct cgcccacgtt gccgtcggcg tggtagtagg tggtcggcag gaagtcgtcg
     2101 atgaacacgc cgtcccacgg cgagccgcgg gcggtgatct cgcggagcac gttcgacgcc
     2161 caggcgtcct gataggccgg gttgccgacg tccgcgaagt agtagtaggg cacgctcgag
     2221 gaggcgagtg gctggccgtc gccgccccgc agcagccagg agggttttgc cttcgcccaa
     2281 ggaacgccgg agaccgactt gccggtggcc ttgtcgcttt cggtcgagac gaacgtctcc
     2341 ttgtagacca gcacccgcag tccggggttc gctgccttca gcttcgccgc gatctgatcc
     2401 ttccagggcg ggagcacgat cagtgcgttc cggcgggccg tcgcggcgag ctgtgcgtcg
     2461 ttcgcgtcga cgagctcgtt gctgccgatc gcgtagaaga agtggtggag cgcgccggga
     2521 tagaccgtgt cgaacgagtc tgaggcgaag gggagcatcc cctcgtcgga tggcggcggc
     2581 tgctctcggt aggtcgtgcc gggcgtgatg ccgggaatga tgggcagcga acctgggagc
     2641 cgaccgggca gatccgcact tgccggtttc gccacacctc ccacgagcgc agcacaggcc
     2701 agagcagtga gcatcccgct cctcatgctt acttcgacgc tacccggggt ttgtcgcctt
     2761 caggctgtgg ccggcgcgcc agttacgagc cagcgccgtc cctggaagcg cgcgaggccg
     2821 gtcacgaggc gaacggcgat cagcgcgatc agggcggtcc agactcccgc gataccgaga
     2881 tcgagctggt acgtgatgag cgccaccatc gcgaagcagg cgaaggcggc caccatcgcc
     2941 cacttgatga agcgtgtgtc gcccgccccg atcaggatgc cgtcgagcgc gaacacggca
     3001 ccggcggcgg gctgcatgat cgcgaggaag ggccacatga cgtgcgcgcg gtcgatcacg
     3061 cgcgggtcgt cggtgaaagc gtgcgggatg acggccacca gagagccgag cacgacggcg
     3121 aaggcgagcc cgacgacgac ggaccagccg atcatgcgcc gcgcggaggc gtaggcctcg
     3181 tcggcgccac cggcgcctag cgtacgcccc acgatcacct gtccggcgat cgcgatcgcg
     3241 tcgagcacga gcgcgaggaa cacgaagagc tggaagccga tctggtgcgc gccgagcgat
     3301 gccgagccga cgcgcgccgc cagcgcgctg gcgagcagga acgaggcata gagcgccgtg
     3361 gtcctcaccg cgatgtcgcc gccaacgcgc agcagcggcc gcagatacgc gaggttgggg
     3421 cggcgcgtgt cggcgggcgc gcgcagtagc gcggcggcga acgccgaggc catggcgagc
     3481 tgggcgatca ccgtccccca ggccgagccc gcgatgcccc agtcgaacac gtatacgaac
     3541 acgacctcga gcaccacgtt cgcgacgttg gcggcgatca cgatgactag cggcagccgg
     3601 aagtcggaca ccccccgcag atagccctgg cccgcgaggg cgaccatcgc gcagggaagc
     3661 ccaatcgccg agatgcgcag gtagagaacg gcgaggtccg cgacccggcc gtctccgccc
     3721 atgagggaga ccgccggacg cgccagcccg accgcgagcg cggcgagcag cgcgccgatg
     3781 ccgagcgcga gccagagcgc ctgggcggca agccgcccgg cgatctcctc ttcgcccgcg
     3841 ccgtggtagc gggccacctg cgcggtggtg ccgtaggtga ggaagttgaa gatcgtgaag
     3901 ctgccggtga tcagcgtcgc cgcgatcgcg agtgcagcca actggtcggt gccgaggtgc
     3961 ccgacgatcg ccgtgtcggc gaggacgtag agcggctccg ccgcgagcgc cccgagagcc
     4021 ggcagcgcca gccgcaggat ctcgcggtcg actgctcggg cgctacccgc gctcaaccct
     4081 gctgcccggg acgatgtcgt ccttgccgga ctcgtcccag cgcacccacg ccttgtgccc
     4141 gccgtcgtag tcgggcgccc agagcacgag cgtcgcgccc tcgccatcca cgctgcagcg
     4201 gtcggatccc tccgggtcct cgccgcgcca gatgcgaatg aaccggttgt gctcccactc
     4261 gtcgccgatg gtggtggggt ccgtgtgccc cgggtggacc gtcacctcgt gcggcagctt
     4321 catcagcctg tccatgatcg aggagcggat gtcctcgaag ctggtcgagc ccggtgcgcg
     4381 cacgccgccg accgagccct tgaagagcgt gtcgccggtg aacacgtcgc tgccgttcac
     4441 gaagaagttg agcatgccgg cggtgtggcc gggcgtgtgg atggagtcga tcgacaggcc
     4501 gccgacctcg agcgtctcgc ccggctcgat cgtgcggtcc acgtcggcga ggaactcggc
     4561 ctcgagcggg tgcgcgagga tctccacccc gtagcggtcc ctgtaggcct tgttctccgc
     4621 aacatggtcg tggtggtgat gggtgagcag caggtgccgc accgtgagcc cgtggcggtc
     4681 gatcgcctcg atgatcggct cagcggggcc gccggagtcg atcaccacgc cggcgccgcc
     4741 ctcttcgtcg gcgacgagat agctgttcga gagccagtcc ggctgcatcg agcgctcgac
     4801 gatcacggcg cagagcatac gatccgccgc tcgtgcccgc cgctgacgca gccgccttcc
     4861 gagccgagtt cccggtgctc gagcgcatcg cctacctgaa cacgggcagc gtgggtccgg
     4921 tgccgagccg tggtgcggcc gcggcgcgcg agcgaatcga caccgagctg ctcgagggac
     4981 gcatcggcca gagcgcctgg gagggcctgc tcgcgctgag cgagcggctg cgggaggcct
     5041 acgcgcgcgt gctgggcggc gacatcgacg aggtggcggt cacgcgctcg acgaccgacg
     5101 gctgcaacgc cgtgatctgg gcgctcgagc tcggcgaggg cgacgaagtg ctcacgagcg
     5161 atgaggagca tccgggcctg ctcgcgccgc tcaaccaggc gcggcgccgt cgcggcatca
     5221 gcgtgcgcgt ggtgcccttc gccgagctgg cttcggcgat cacgccgcag accaagctcg
     5281 tcgcctgctc gcacgtgtcc tggctctcag gccaggtcgc ggacgtcgag gcgctcgctg
     5341 ccgcggaccc gatggtgctg ctcgacggcg cccaggggct cggcgcggtg ccggtcgacg
     5401 tgcccgcgct cggctgcgac ttctacgcgg cctctggcca gaagtggttg tgcggcccga
     5461 actcgagcgg ctgcctctgg gtgcgctcgg aacggctcgc ggagatgtcc acgccgtggc
     5521 cgatcttcga atcggtggtg gatcccgagc acgcgctcga cagtccgatg cgagaggacg
     5581 cccaccgctt cgattccggt gtgctcgcct cgtcgtccgc ggcctggtcg ctcgccgcca
     5641 tcgagctgtt cgaggagtac ggcctcgcct ggatgcagga gcgctccggc tcgctggccg
     5701 agtggctcgc caacacgctg cgggagcgcg ggctcgacgt gatgccgcgt gggcgctcga
     5761 cgctcgtgag ctggcgcgac ccggatccgc cgacgaccgt tgagcgcctc gccgcgcagg
     5821 gcctcgtgct gcgccacatc ccaggcgccg gcgtggtgcg cgcgtcggtg ggcgcgtggt
     5881 cgagcgagca ggagctcgat cggctcgtgg atgcggtcgg ccgctagctc agggggcgcc
     5941 ggtactccac gagctcgagc ccgagctctt cttgcacgaa gggctcaccc agcgttgtcc
     6001 agccctcgcg ctcatagaag cgccgtgctc gcgcctggcc cgaaggcgtg tagaggcgaa
     6061 tcgaggtgaa gccgcggcgg gcggcctccg ccatcgcctc gaagtgcagc gccgtggcga
     6121 tccccgtacc ccaatacggc tcccgcacga agagctgcca gaggtgcgcc agtccggcct
     6181 ccgggctgcc ccagcgggtg ctgcgcgccg gcgcgaacgc cacgtgcccg gccacctcgc
     6241 cttcgcgctc ggcgagcatc gcccacacct cgggatagga caagcgctcc gttatcgcca
     6301 tgaagtcgtt cacgtctgcc ggcgggtccc agccctccgg ggcgaaagag cggtagccct
     6361 cgaagccctc gcccaccgtt cgggcgagct cctcggcatc cgcgggagtg gcgaggcgga
     6421 gcgtgaagcc cggctcgttc aggtcttctt cgtcgtgtag tagatgccga cgctgatcgc
     6481 ggccaggcag acgaggaccg ccacgcccgc gagcacgccg tacgcggtcg cgtgggacct
     6541 gcgcccgtcg cgcgccagct cataggagcg cgtcgctccc gcgatggcga tcgagaacgc
     6601 tgtggtcact ccgatcccgc cgacgagcga gtacagcacc gactcgccga gcgtcttcca
     6661 ttcgacgacc gtggcgatca cgtgcgcgcc tccgcggtga tcggcgagcc gcgctggacg
     6721 cgcctcacga agatcacggt gagcccgacg aggatcagga tcgccacgac gatcggtccc
     6781 gtggccccgg taccgaacag tcgggtgatg ccgtagaccg ccgcgccgat cccgccggca
     6841 gccggcagcg tgaggatcca ggcggtgacc atgttgcccg cgactcccca gcgcacggcg
     6901 gagacgcgct tcgccgcccc cgcccccatc acgccgccgg agatcacgtg cgtggtcgag
     6961 agcggatacc cgaagtggga cgaggcgagg atcaccgccg cgcccgcgcc ctgagcggcg
     7021 aagccctgcg ccggatccat cttgatgatg cgcatcccca tcgtccggat gatgcgccag
     7081 ccgccgctgt aggtgccaag cgcgattgcg gcggcggcgg agacgatcac ccaggtgggc
     7141 ggatttgcgc cttcggggag gttgccgttg gcgatcagcg cgagcgtgat cacgcccatc
     7201 gtcttctgcg cgtcgttggt gccgtgggcg agcgcgagga gaccgccgga ggcgacctgc
     7261 ccgatccgga agccgttcga caccggtccg ggacgcagcc gcccgatgat ccggtacgcc
     7321 agcaggatgg acaccgcggc cgtgaggaac gcgaccgtgg gcgcgacaag cgcggggatc
     7381 acgaccttgc cgaacacgcc gtcggcgctg accgcgctgg cgcccgccgc cgcgagggtc
     7441 gccccaatca tcccgccgat cagtgcgtgc gacgagctcg acggcagtcc gtaccaccag
     7501 gtgatcaggt tccaggcgat cgccccgacg aggccggcga acacgaccgt cggcgtgacc
     7561 gcgccggcgt cgacgatgcc cttcgccacc gtggccgcga ccttgatcga gatgaacgcg
     7621 cccgcgaagt tgaggagcgc ggcgaagagg accgccagcc gcggaggcat cgcgcgcgtg
     7681 gagatcgacg tggcgaccac gttcgccgtg tcgtggaagc cgttcgtgaa gtcgaacagc
     7741 agggccgtcg cgacgacgat gactaggacg atgtcgttgc tcacggccgg ctacgagttc
     7801 ttgatgacga tcccctcgag gatgtacgcc acctgctcgg tggcgtcgat cgcggcctcg
     7861 agccggtcgt agatgtcctt ccagcggatc acgaccatcg ggtcggtgcc ggtctcgaac
     7921 agcgaggcga gcgcctcgcg gtgaatccgg tcgccgtcgt tctcgaggcg gttgagctcg
     7981 accgtgtagt gcgagatgtc gttgaagccc ctgagccgcg gcatcgcctc cgcgatcagg
     8041 cgcccggact gaacgagcac cttcgcgagc tgctgcgcgg gctccatcgg cgcctcgatc
     8101 ttgtagaggc cgaggaagtc cgcggtctct tcggtgaagt cgaggatgtc gtcgagcgcg
     8161 gaggcaagct ggtggatgtc ctcgcggtcg atcggcgtaa cgaaagtgtt gttgagtcgc
     8221 tggatgatgt catgcgtgat gcggtcaccg tgctgctcgc agatgaggat ctcgcgggcc
     8281 agctcggctt gctcggggta gtcgcgcagc atctgctcga gcagggccgc ggcccgcgcg
     8341 atgttgccac cggcctcctc gaagaggtcg aagaactcgc ggtcgcgcgg aaggaaggcc
     8401 tgtgacaggc gtgccatcta atgggtcctc tggtggtcga tgtgacgggc ggcgggggct
     8461 gcttgccctg gcgcgccccg gcggcgcgcg cagtgtaagc cctttcacac ggtttttaca
     8521 tctacgcctc gacgtcaaag cgagcggcct cgaggcgaag ggcggtcggc tcccccagct
     8581 cgcccatccg cgctcgggtg tccatcgccg ctgcgtccga gtcgtacccg acccggcgca
     8641 gctcgattcc gccgttttcg tcgatcagcg catacgccgc gcgccggtcc ccgtcgaatg
     8701 gaagtccaac gctgcccggg ttcagcatca ggatcccgtg ctcgctcgtg cggctgaact
     8761 gcaggtgggt gtggccgaac accagcgtcg gctcggcgac cccgtcgatc agttcgtcct
     8821 cgtcctccgc cggctcgggg aagaagctgc gcatgtctga ctgtggcgag gcatggcagt
     8881 agagcgctcc ctcgtacacg cgcgcctgct cgagcgccgc gagctcggcc acgaggttcc
     8941 cgccgagcgc gccctccgcg taggcgatcg cgcgctggag ccattcgtcg tcgggagcgt
     9001 cctcggggtg cgcctgccag cgctcccagt tgccgcggat ccaggtggcg ttctcgagct
     9061 gctggaggcg cgcgagcgtc tcctccggcc acgcgcccat cccgacgtag tcgcccccga
     9121 ggaagaagct gtcggcgccg acctgctcgg catcctcgag gaccgcctcg agcgccggga
     9181 ggttgccgtg aacgtcgtag acgacggcga gcattagaac aaggtctcgc cggcgcgcgc
     9241 gccgcgtcgc cgcgggcgcg gcactccggc cgcctcgacg agcgccgcct ccacgtcctc
     9301 gaggatcgcc ttctcggaac gcgacgccac gatcggctcg ctcagcccgt ccacgtgcac
     9361 gtaggtgcgg cgtccgtcgc cccgctccgc cggctcgagc gggaccagcg cgaacacgcg
     9421 gtccgctctc acgaacttgc cgtacccgag cgcgaccatg cgcgcgtctc gcttcgctgc
     9481 cattcagcct cctagctcga gcggggcgat cacctagcag cgtcaagcat ctcacctgtc
     9541 ccaggcgtca agcgctggaa gcgcgagccg atacaggaag catgatgctc agcgcctgcc
     9601 tcctgttcac ggctctcgtc gccatctggg gctcgcccac gccacgcctg ctcaaggagt
     9661 tgttcgagca gtcccgcgtc gagggcgacc agccggccga gccggttctg tagccccaaa
     9721 cagcagcaat aagggtggcc tggagcgggt tcgccggctc caggcggacg agactgattt
     9781 tgagccccta tatggggccc aacttaaagg tcgtttcgcg aaccttttcg gagcggggcg
     9841 aaaccaccca tcgggctccc gttagtgctg tcgtagacgc gacgacaacc gcgccgcccc
     9901 ggagctactc cccgggcacg tccagcacga ccttcccggt cgcgccgcgt gagtcgatga
     9961 gcttcatcgc gtctgccgcg cgcgccagcg ggaagcgcgc gcccacgatc gggttcacgt
    10021 acccctcgtt gacgagaagc tcgatctccg cgccgatctg gcggttcagc tctggcttgc
    10081 tcatgacgta ggcgccccag cccgccccca ccacctcggt gttcttgagc agcaggcggt
    10141 tcaccttcac ctccggaatc gagccgccgg tgaagccgac caccacgagc cgcccgtcct
    10201 cgcgcagcga gcgcaggctg tcggtgaagc ggtcgccgcc gaccgggtcg atcacgatgt
    10261 ccacgccgcc acccgacagc tcgaccacct ggtccttcca cgggccatcg gagcgcacca
    10321 cctcgtccgc gcccgcctcg cgcgcgacgc gctccttctc atcgctcgac acgaccgcga
    10381 gcgtcttggc gccgagccct ttcgccacct gcaaagtggc cgtcccgacg ccgcccgccg
    10441 cgccgtgcac gagcacctgc tcgccgggct cgagcctccc tcgcagcttc agcgagaagt
    10501 aggcggtgtg gtagttcagg atgagcgccg cgccctgagc gagatcgagc tggtcgggca
    10561 gcttgaacgt gagcggcgcc ggtgcgacga cgcgttccgc cataccgccg agcatcgtca
    10621 tcgcggcaac gcggtcgccg ggcttgacgt ccgcattccc cgatacatca acgaccgtcc
    10681 ccgccacctc ggctcccggc acgaacggca ggtccggctt gaactggtag agcccgcgcg
    10741 actggagcac gtcgggaaac gacacggccg ccgcctcgac gtcgatcagg acgccctcgc
    10801 ccggcgtgag catgtgcgag gcgccgggct ccggcacgtc gaccacgctg agtccgtcgg
    10861 ggccggtcag ctcggagatc tggagtgcgc gcatgcggcg gcactgtagc cgccaaagag
    10921 aaaaccccgc acgcggcggg gctcccgagt gggccgtact gggctcgaac cagtgacccc
    10981 cagcttgtcg agctggtgct ctcccaactg agctaacggc ccttctgcgc ggcgagcata
    11041 gcagcggccc ggggcccgcc gcgaccctct caggcgccca agggcgcacc cacgttgacg
    11101 ctcagcgaag cgccttcgcg gggcagtttg cctcgtgctc gcatcgcctc ctggaacgcc
    11161 tcgaggctcg agtagcgggg ctcccagccg agcgtcgcct tcaccttctc gttcgacgtg
    11221 acccacgggt gaatcgcgaa gtggaggttg ccgggcggcg tctccgcctg gcgcagcttc
    11281 cacatcaggc cggcgaagcg ccagaacagc ttgagcggaa ccttccgcct cggcagcccg
    11341 atcatctcgg cgagctgggc gccggggatc acaccgtcgc cggcgacgtt gaacgcgccg
    11401 gcgtgccgac cgacgatcag gccgatgatc gcgtcgacca ggtcgtcctc gtggacgaac
    11461 tggaagtcca cgtcgggcag gccgaagtcg gcctggaacg gctgatcggt ccagagccgc
    11521 acgatgtagt tgtccacgtt cgggcccatg acgatgcagg gccgcacgat cgtcatcacg
    11581 cggtcggggt tgcgcagcgc ccacagctgg cagagccggt cgcactcggc cttgtcgcgc
    11641 gcatattcga agtccggcac gcctcgcacc gggtggtcct ctgggatggg attcgggttg
    11701 tcggggaacg cgccgtacgc ggtcgtcgag gacgtcacga gcagctgctc gatgccggcc
    11761 ttctccgcgg cctcgagcac gttctgagtg ccaccgacgt cgatgtcgta catcgcgttc
    11821 tcgtcatgca tcggattgag gatgaacgcg aggtgcacga gcacgtccgg cttctcgcgc
    11881 gccagcgtgg cgcccaccgc caccttgtcg cgaacgtcca tctgcacgta ctcgaccttc
    11941 gggttgaacg tgcgcggcgg tctgacgtcg gcgacgacga tgcgatcggt gtcgtcccgt
    12001 tcgagtagcc gctgcacgag ccggctgccg atgtagccgg agcctccggt gaccaggtaa
    12061 cgcatggatc gagtcctcct cctccgcggg cgtgcgcgcg atcctatccg cgccgctccg
    12121 acacgcgcag cgtcttcacc acccacgcgc agcccggtgc aaccttgtcg cggcggttcg
    12181 gcggccactg gcagctcacg acagtcagct gccctcgcgg agcgagcacg aaccacgcct
    12241 cgccgtcgag gcggtcgccg cttcggaccg aacgcccctg tatgtcgaag cgcagcgcgg
    12301 gcctcccggc cattcgcgcg tgcgcgggct cgggcacctt gccgtccacg tcgcggaagc
    12361 gcggcgccac gaacgcggcg agctcgtcca cgttccgcgc tccggtcgag tcgggcacct
    12421 cgtgcaccac cacgccgctc ttcgagtcac ccgtgagccc gacggccgtc gtatgcgact
    12481 cggactgcga ggcaagcttg ccgcggaccg gctcctccac gaagcccggc ggcaccgtgt
    12541 agacgtagtc accgcccacc gcgcgcacct ccttgccgcc cgagctgccc gcgggcttct
    12601 ctccgccgcc acaaccggcg aggacagcgg ccgcgacggc gaat
//
LOCUS       DASVXS010000280        11428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_693757, whole genome shotgun sequence.
ACCESSION   DASVXS010000280 DASVXS010000000
VERSION     DASVXS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693757"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF752_15820"
     CDS             complement(<1..402)
                     /locus_tag="VF752_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857061.1"
                     /translation="MRTAPATLPSRDVRSRKPLLLRMETARILLRVGSLLLLDAAGLF
                     LAIWTALELKAVARGKSDLVLSFHQTVDVAPLAILVTILLFARSGLYGDRGVRPGFSR
                     VVASLFQVTFVILIYAIAEGQHFSSYYIFYGS"
     gene            609..755
                     /locus_tag="VF752_15825"
     CDS             609..755
                     /locus_tag="VF752_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857062.1"
                     /translation="MRTRPRRRHYVRTSVLRVARVAFRYSRTRDAYVLRGIGSHIGPV
                     LKRR"
     gene            complement(752..2056)
                     /locus_tag="VF752_15830"
     CDS             complement(752..2056)
                     /locus_tag="VF752_15830"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8857063.1"
                     /translation="MANDRKNADGGRTRRAAVLGGNRIPFARSNGPYAHASNQDMLTT
                     ALDGLVARFNLQGERLGEVVAGAVLKHSRDFNLTRECLLGSALDHRTASYDVQQACDT
                     GIQAAIAVANKIALGQIESGIAGGVDTTSDAPIAVNDDLREVLLDFNREKSATGRLKL
                     LGRIRPGQLVPEIPRNAEPRTGLSMGEHQAITTREWGITREAQDELAAASHRNLAAAY
                     DRGFFDDLVTPYLGLDRDQNLRPDSDPEKLAKLKPVFGKGEDATMTAGNSTPLSDGAA
                     AVLLASDEWAEQHSLPVLAYIVDAETEAVDYVHGDDGLLMAPVYAVPRLLARNGLTFD
                     DFDFFEIHEAFASQVLTTLAAWEDPAFGKERLGLDGAFGTLDRSRLNVNGSSLAAGHP
                     FAATGGRIIATLAKMLAEKGSGRGLISICAAGGQGVVAILER"
     gene            2093..3514
                     /locus_tag="VF752_15835"
     CDS             2093..3514
                     /locus_tag="VF752_15835"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX8857064.1"
                     /translation="MGTNQYVMTDRYRQFTESPPGRFLARQLGMPRPASLRRYEPGQP
                     LLAGPALLGAAPGGRLTGAVASVLSAAGAEAHVPKQGEVRDAALEAGLETQVSPPQDD
                     DRRYGAIVFDASGMKSSDQLQSVYEFFHPVMRRFTESGRLLILGTPPASCADAREATA
                     QRALEGFLRSAAKELSGGATGQIVYVDPGGEGNLESTLRFLLSAKSAYVDGQPVFVGA
                     GESAAPVDWERPLDGKVAVVTGASRGIGEAIAEVLARDGAHVVALDVPAQGDALAEVA
                     NRVSGEALQLDITSPDAPQELGDHLRERHGGVDIVVHNAGITRDKTLGRMSEDQWASV
                     IAVNLTSQERINDALLSGDVLRRGGRIVSVSSQSGIAGNRGQVNYAASKAGVIGMVHA
                     LAPQLAERGSTINAVAPGFIETQMTARMPVVTREAGRRMNSLAQGGQPVDVAETIAWL
                     ASPGSGGVNGNVVRVCGQSLLGA"
     gene            3514..4425
                     /locus_tag="VF752_15840"
     CDS             3514..4425
                     /locus_tag="VF752_15840"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8857065.1"
                     /translation="MAMRELSSPPSLTSLYPKALAGSTVQPVLGHLPGLGGRFKRGRE
                     LPDEELVLTDAGIEREHLREYDRVCGFTLRDELPATYPHLLAFPLSMELMTSTSFPFP
                     VIGLVHIANRIEQLRPIRDDERLTVRVRTEDLADHERGTQFEIAADGEVAGETVWRSR
                     STYLHRSGGGDDGKKRRAESPKLPEPAAIWEIPGDIGRRYAAVSGDRNPIHLHPLTAK
                     LFGMPRPIAHGMWLKARCLAALEGHLPDAYAVEMRFKLPVYLPSKVSFRSWSEGSAHR
                     FALHDANGEKPHLEGETTALGDSAVAE"
     gene            4649..5527
                     /gene="nudC"
                     /locus_tag="VF752_15845"
     CDS             4649..5527
                     /gene="nudC"
                     /locus_tag="VF752_15845"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX8857066.1"
                     /translation="MSPPNTFAGAALDRASAKRTDEAWLGERIADPASRALPVAPEGP
                     YLRCEGDDCAAALVPLTEVADQARELILLGVDSHGALFAATLDSAPGIDGAAPLGLRE
                     AAFRVGQADGGLLAYATALANWHRRHRFCANCGNATHTAEGGFVRTCPRCGAVHHPRT
                     DPVVIMLVEDGERVLLGRQPSWPAGRYSALAGFVEPGESLEEAVAREVREESGVEIEP
                     PRYSSSQPWPFPASLMLGFHAVYAGGEPHTLDSELDDVRWFTREEVDLAAQGEGPFHL
                     PPPIAIARRLIDEWLS"
     gene            5515..6465
                     /locus_tag="VF752_15850"
     CDS             5515..6465
                     /locus_tag="VF752_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8857067.1"
                     /translation="MAQLTGTIPHRSIGSLSVSAVGLGGNQFGGRLDLDGTRAVVEAA
                     LDAGIDFLDTADVYGNKGGSERLLGEVLAGRRDEVVLATKFGWDMAGANGEPAGAQGS
                     RAYIRQAVEASLKRLRTDVIDLYQYHRPDGVTPIEETLGALDELVREGVVREIGCSNF
                     SAAQLEEAERVARERGYPRFVSLQNEYSLLEREIEADVVPLCERYGVAVLPYFPLARG
                     LLTGKYHRGEPAPEGTRLHGREQLADDATWDKIEALKRFAAGRGISMTDLAIAGLLAR
                     PIVASVIAGATKPEQVRSNAAAVRWQPTDQDLAEFEAILR"
     gene            6570..8726
                     /locus_tag="VF752_15855"
     CDS             6570..8726
                     /locus_tag="VF752_15855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8857068.1"
                     /translation="MPEPPGVAPPGPLTERTAREVKERRGAGSTRPGRSATPQPPAAD
                     LTDWPELDAVRLSHEHWRRRRARHRGPLRRTAAIAGLALLAVAAAITGSLLLLAGDPG
                     LAVGCNLASAHPRVVGRDTFIRAENGMVLGAVPTAQNREPVPLERMSPWLPKATVAIE
                     DRRFWQHGALDYDAILRAAIADLKAGRVLQGGSTITQQFVRDRYLAGQGATLHRKLKE
                     ACLAVQLAHEWPKRRILQAYLNLVFYGGQAYGAEAAARTFFGRSANSLTLAQAALLAG
                     LPQAPSVYDPFARPVAARARRNEVLRAICKAGYISTARYRSASSSPVRLHPSARYTRI
                     LAAPFFNYVTRELVRRLGAQRARRGGLTVETTLDPRLQRLANKAISGWLYKPSDPAAA
                     LVAIDPRNGAVRAMTFANPGRAALAFNLASQSHRQAGSAFKVFTLTAAMERGIALNSV
                     WHGPASLTIPNRSCLNANGPWVVHNFADEATGTMTLLRAIAHSVNTIFAQVIMRVGPA
                     NVVDVAHRMGIRSHLKPVCALTLGPEGVSPLEMTTAFATLAARGIRHRPQPLSAVLGR
                     GGRALMRLDANGKRALSQSVVDRVTYALTGVVRAGTGRAADPGRPAAGKTGTAENFKD
                     AWFCGYVPQLATCVWIGYPQAEIPMHGVGGFAQVVGGSVPARIWHDFMLPAMAGQPVR
                     GLPTASPGRVGPAPQAPPLPPLSPPVPATITPALPR"
     gene            complement(8732..9193)
                     /locus_tag="VF752_15860"
     CDS             complement(8732..9193)
                     /locus_tag="VF752_15860"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX8857069.1"
                     /translation="MTALTDYLDAAGVDYEVVEHRRTVTAAEDAQAVGARAADEAKTV
                     LLRIGGSYGLAVVPASERLDLHKLRARVGQGEELRLASEDEMAAEFPEYELGALPPVG
                     PTMPSLEVMDPRLLDCERVACTAGDHTHSLWMKPNDLTRLADPIVADICQD"
     gene            complement(9281..10405)
                     /locus_tag="VF752_15865"
     CDS             complement(9281..10405)
                     /locus_tag="VF752_15865"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8857070.1"
                     /translation="MLKKLALVAALVLCLAPAAAQADEGNAEGHVAKVMTRNLFLGAD
                     LAPAIGAPNLTAFVNANGGILRQVTATNFPVRAKGLAAEILGQEPDLVGLQEVALWRT
                     AAPSIAPVLGGPKTAETVRYDFLQLLLDELNKDGTKYEAVVVQPEFDLEAPANENNVS
                     GDGPFPIVNAELNARLTMRDVILARVDNSSVKVANPQAGHFANPLVLPILGNPVTVTR
                     GWTAIDAKVRGSGWFRFVNTHLEAFDPRSVVPSIRAKQAGELVAPGGPASGDLPVVLL
                     GDFNSDDDTVFNGDQQAYNVLTGAGFAERSTNDPLSCCVHSANLQTGTDAEFDHQVDH
                     VLTNAPDEVQLVDSAVTGREMQNGYWDSDHAGVFSALRIK"
     gene            10590..>11428
                     /locus_tag="VF752_15870"
     CDS             10590..>11428
                     /locus_tag="VF752_15870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8857071.1"
                     /translation="MTQETLRHGSWAGARTRPTRAEIDPAALELIERYGASLLRTARR
                     YSLSAEDAEDAYQRGLEILLTKAPSTSEADLLPWLKTVVKHEAFAIRRQRQRAELRDD
                     DSSWDGATASTDEQAERFERLQIGAEALGRLKPQETRALLLKAQGLSYQEICAETGWT
                     YTKVNRCLTEGRRAFLDRVAGIESGDECDRLAPQLSALADGEASAEDMALLRPHLRSC
                     LYCRGALREYREAPARVAVLAPAGLLTLDSMDEAAERSWSLLGWLHDRFMLAWMRAHE
                     AALS"
BASE COUNT         1677 a         4007 c         4043 g         1701 t
ORIGIN      
        1 cgagccatag aagatgtagt aggaggagaa gtgctgaccc tcggcgatcg cgtagatcag
       61 gatcacgaac gtcacttgga agagcgacgc gaccacgcgc gagaagccgg gccgcacgcc
      121 gcggtcgccg tagaggcccg agcgcgcgaa cagcaggatc gtgacgagga tcgccagtgg
      181 cgccacgtcg acggtctggt ggaacgacag cacgaggtcg gacttgccgc gcgcgaccgc
      241 cttgagctcg agcgcggtcc agatggcgag gaagaggccc gccgcgtcga gcagcagcag
      301 cgagcccacg cgcaggagga tgcgcgcggt ctccatgcgg agcaggagcg gcttgcggct
      361 gcggacgtcg cgggacggga gcgtcgccgg agccgtcctc acctcgcgca gttctcttgc
      421 caagccgttc gccatccgcc ctgcctgtcg aaaccggtcc gctgcgcaaa tgtatcggtg
      481 gacgcacgcg cacgggaatg cccattaggg cagcgaaccg ccgctgaagg ttagaggttg
      541 gctaaagcct cttgcacgtc agccccggcg gtgagagctt ggggtacggg gagggcgaga
      601 ggtcccctat gcgaactcga ccacgtcgcc gccattacgt ccgcacgtcc gtactgaggg
      661 tcgcccgcgt cgccttccgc tacagccgca cgcgcgatgc ctacgtgctg cggggtatcg
      721 gcagccacat cgggcccgtc ctgaagcgtc gttagcgctc gagaatcgca accacgccct
      781 gcccgcccgc ggcgcagatc gagatcaggc cgcggcccga tcccttctcg gcgagcatct
      841 tcgcaagcgt ggcgatgatc ctgcccccgg tcgccgcgaa cgggtggccg gccgcgagcg
      901 aggagccgtt cacgttcagc ctcgagcggt cgagcgtgcc gaaggcgccg tcgagcccga
      961 gccgctcctt cccgaacgcg gggtcctccc aggcggccag cgtggtcagc acctgtgacg
     1021 caaacgcctc gtggatctcg aagaagtcga agtcgtcgaa cgtcagcccg ttccgcgcga
     1081 gcagccgcgg cacggcgtag accggcgcca tcagcaggcc gtcgtcaccg tgcacgtagt
     1141 cgaccgcctc agtctcggcg tccacgatgt acgcgagcac cgggaggctg tgctgctcag
     1201 cccactcgtc gctcgccaga agcaccgcgg ccgcgccgtc cgacagcggg gtcgagttgc
     1261 ccgcggtcat ggtcgcgtcc tcgcccttgc cgaataccgg cttcagcttc gcgagcttct
     1321 cgggatccga gtccggccga aggttctggt cgcggtcgag gccgaggtag ggcgtcacca
     1381 ggtcgtcgaa gaagccgcgg tcgtaggccg ccgcgaggtt gcgatggctg gccgccgcca
     1441 gctcgtcctg cgcctcgcgc gtgatgcccc actcgcgcgt ggtgatcgcc tggtgctcgc
     1501 ccatcgacag gcccgtgcgc ggctcggcgt tgcgcgggat ctccggcacg agctggccgg
     1561 gccggatgcg accgagcagc ttcagccggc cggtcgcgga cttctcgcgg ttgaagtcga
     1621 gcagcacttc acgcaggtcg tcgttcacgg cgatgggcgc gtcgctcgtc gtgtcgacgc
     1681 cgcccgcgat gccggactcg atctgcccca gcgcgatctt gttcgccacc gcgatcgcgg
     1741 cctggatccc ggtgtcacac gcctgctgca cgtcgtacga ggcggtgcgg tggtcgagcg
     1801 cgctgccgag caggcactca cgcgtgaggt tgaagtcgcg actgtgcttc agcaccgcgc
     1861 cggccaccac ctcgccgagc cgctcgccct ggaggttgaa gcgcgcgacc agcccgtcga
     1921 gcgcggtggt gagcatgtcc tggttgctcg cgtgcgcgta cgggccgttg gagcgcgcga
     1981 acgggatgcg gttaccgccg agcaccgcgg ctcggcgcgt ccggcctccg tctgcattct
     2041 tcctgtcgtt cgccatgtcg ctccttcgct gaccgtttgt tcagtttggc aaatgggcac
     2101 gaaccagtac gtgatgaccg accgctatcg acagttcacc gagtctccgc cgggccgctt
     2161 cctcgcgagg cagctcggca tgccgcgccc cgcctctctt cgacgttacg aaccgggtca
     2221 acccctgctc gccggccctg cgctgctcgg cgccgcgccg ggcgggcggc tcaccggcgc
     2281 ggtcgcgtcc gtgctgagcg cggccggcgc tgaggcgcat gtgccgaagc agggcgaggt
     2341 ccgcgacgcg gcgctcgagg ccggcctcga gacgcaggtg tcgccgcccc aagacgacga
     2401 ccgccgctac ggcgcaatcg tgttcgacgc gagcggcatg aagtcgagcg accagcttca
     2461 gtcggtctac gagttcttcc atccggtgat gcgccggttc accgagtccg ggcggctgct
     2521 cattctcggg acgccgccgg cctcgtgcgc cgatgcgcgg gaggcgaccg cgcagcgcgc
     2581 gctcgagggc ttcctgcgtt ccgccgcgaa ggagctctcc ggcggcgcga cagggcagat
     2641 cgtgtacgtg gacccgggcg gcgaagggaa cctcgagtcg acgcttcgct tcctgctgtc
     2701 ggccaagtcc gcctacgtcg atggccagcc ggtgttcgtc ggcgcgggcg agtccgccgc
     2761 cccggtggac tgggagcgcc ccctcgacgg caaggtggcc gtcgtcaccg gcgcctcgcg
     2821 cgggatcggc gaggcgatcg cagaggtcct cgcgcgcgac ggcgcgcacg tcgtggcgct
     2881 ggacgtcccc gcgcagggcg acgcgctcgc ggaggtagcc aaccgggtta gcggcgaggc
     2941 gctccagctc gacatcacat cgcccgacgc gccgcaggag ctcggcgacc acctgcgcga
     3001 gcgccatggc ggcgtggaca tcgtcgtcca caacgccggc atcacgcgtg acaagacgct
     3061 cgggcggatg agcgaggacc agtgggcgag cgtgatcgcc gtgaacctca cgagccagga
     3121 gcggatcaac gacgcgctgc tgtcgggcga cgtgttgcgc cgcggcggcc ggatcgtgtc
     3181 ggtttcgtcg cagagcggca ttgccggcaa ccgtgggcag gtgaactatg cggccagcaa
     3241 ggcgggcgtg atcgggatgg tgcacgcgct cgcgccgcag ctcgccgagc gcggcagcac
     3301 gatcaacgcg gtggcgccgg ggttcatcga gacgcagatg accgcgcgca tgccggtcgt
     3361 gacccgcgag gcggggcggc gcatgaacag cctcgctcag ggcgggcagc cggtcgacgt
     3421 ggccgagacg atcgcctggc tcgcgagccc cggctccggc ggcgtgaacg gcaacgtggt
     3481 ccgcgtctgc ggccagagcc tgctgggcgc ttgatggcca tgcgcgagct gagctccccg
     3541 ccgagcctca cgtcgctcta cccgaaggcg ctcgccggct cgaccgtgca gcccgtgctc
     3601 gggcacctgc ccggcctcgg cgggcgcttc aagcgcgggc gcgagctccc ggacgaggag
     3661 ctggtgctca ccgatgccgg catcgagcgc gagcacctgc gggagtacga ccgtgtctgt
     3721 ggcttcacgc tccgcgacga gctgccggcc acctatccgc acctgctcgc cttcccactc
     3781 tcgatggagc tgatgacgag caccagcttc ccgttcccgg tcatcgggct ggtgcacatc
     3841 gcgaaccgga tcgagcagct ccgtccgatc cgcgacgacg agcgcctcac cgtgcgcgta
     3901 cggacggagg acctcgccga ccacgagcgc ggcacgcaat tcgagatcgc cgccgacgga
     3961 gaggtcgccg gcgagacggt atggcgcagc cgcagcacct acctgcaccg atcgggtggt
     4021 ggcgacgacg gcaagaagcg acgcgccgag agcccgaagc tgcccgagcc ggcggcgatc
     4081 tgggagatcc cgggcgacat cgggcggcgc tacgcggcgg tctcgggcga ccgcaacccg
     4141 atccacctgc atccgctcac cgcgaagctg ttcggcatgc cgaggccgat cgcacacggc
     4201 atgtggctga aggcccgttg cctcgcggcg ctcgagggcc acctgcccga cgcctatgcc
     4261 gtcgagatga ggttcaagct gccggtgtac ctgccctcga aggtctcgtt ccgaagctgg
     4321 agcgagggtt cagcgcaccg cttcgcgctt cacgacgcca acggtgagaa accgcacctc
     4381 gagggagaga caacggcgct cggcgactcg gcggttgccg aatagcgagg cggcgttacg
     4441 gcagcactaa aggtgcgtgc ggccttgggc caccccggcc gtgcggcctc cgcggacgtg
     4501 ggtgacttta tccccgctag atggcggtta aggtcaccca cgcccagacc gggctagccc
     4561 agacgtagta ccacccgcga gccggggccc gccgttatcg ctgcctttcc gtgaaccgcg
     4621 cccaatgctg ctctctaacg ttcagcgggt gagcccgccc aacacgttcg ccggggctgc
     4681 cctcgaccgc gcctcggcga agcgcaccga cgaggcctgg ctgggcgagc gcatcgcaga
     4741 ccccgcctcg cgtgcgctgc ccgtcgcgcc ggaggggccg tacctacgct gcgagggcga
     4801 tgactgcgcc gcggcgctcg tgccgctcac ggaggtcgcg gaccaggcgc gcgagctcat
     4861 cctcctcggt gtcgattcgc atggcgcgct cttcgcggcg accctcgact ccgcgccggg
     4921 aatcgacggg gccgcgccgc tcggcctccg cgaagcggct ttccgagttg gccaggcgga
     4981 cggcggcctg ctcgcctatg ccaccgcgct cgccaactgg caccggcgcc accgcttctg
     5041 cgctaactgc ggcaacgcga cccataccgc cgagggcggc ttcgtccgca cctgcccgcg
     5101 ctgcggcgcg gtgcaccacc cgcgcacgga tcccgtcgtg atcatgctcg tcgaggacgg
     5161 cgagcgcgtc ctgctcggcc gccaaccctc gtggcccgcg ggccgctact cggcgctcgc
     5221 gggtttcgtg gagcccggcg agagcctcga agaggcggtc gcgcgcgagg tgagggagga
     5281 gtccggcgtg gagatcgagc cgccgcgcta cagctcctcg cagccgtggc cgttcccagc
     5341 ttcgctgatg ctcggcttcc acgcggtgta cgcgggcggc gagccgcata cgctcgacag
     5401 cgagctcgac gacgtgcgct ggttcacacg agaggaggtt gacctcgcgg cgcaagggga
     5461 aggtccgttc cacctgcctc caccgatcgc catcgcccgg cggctcatcg acgaatggct
     5521 cagttgaccg gcaccatccc gcaccgctcg atcggctcgc tcagcgtgtc cgcagtcggt
     5581 ctcggcggca accagttcgg cgggaggctc gacctcgacg gcacgcgcgc cgtggtggag
     5641 gccgctctcg acgccggcat cgatttcctc gacaccgcgg acgtctatgg caacaaaggc
     5701 ggcagcgagc ggctgctggg cgaggtgctc gcgggccgtc gcgacgaggt ggtgctcgcc
     5761 accaagttcg gatgggacat ggcgggcgcg aacggtgagc cggccggcgc gcaggggtcg
     5821 cgcgcgtaca tccgccaggc cgtggaggcg tcgctgaagc gcctgcgcac ggacgtgatc
     5881 gacctctacc agtaccaccg ccccgacggc gtcactccga tcgaggagac gctcggcgcg
     5941 ctcgacgagc tcgtgcgcga gggcgtggta cgcgagatcg gctgctcgaa cttctccgcg
     6001 gctcagctcg aggaggcgga gcgcgtcgcg cgcgagcgcg gctatccgag gttcgtgagc
     6061 ctgcagaacg agtacagcct gctcgagcgc gagatcgagg cggacgtggt gccgctgtgc
     6121 gagcgctacg gcgtcgcggt cctcccctac ttcccgctcg cacgtggcct gctgaccggc
     6181 aagtaccacc gcggcgagcc ggcgcccgag ggaacgcgcc tgcacgggcg cgagcaactg
     6241 gcggacgacg ccacctggga caagatcgag gcgctcaagc gcttcgctgc cgggcgtggc
     6301 atctcgatga ccgacctcgc gatcgccggg ctgctcgcga ggccgatagt ggcgtcggtg
     6361 atcgctggcg cgaccaagcc cgagcaggtc cggtcgaacg ccgccgcagt gcgctggcag
     6421 ccgacggacc aggatctcgc cgagttcgag gcgatactgc gctgaggcgc gcgtagcgcg
     6481 cgcgagcgtc gggtaattgc gctctgccac agcgcgaagc gggacaaggg tcccgaggcg
     6541 gcttccgctg agcaggaaga aggaccgcag tgccagaacc gcccggcgtc gcccctccgg
     6601 gccctctcac cgagcgaacc gcccgggagg tgaaggaacg gcgcggcgcg ggttcgacgc
     6661 ggccgggtcg cagcgccacg ccgcagccgc cggccgccga cctgacggac tggcccgaac
     6721 tggatgccgt ccgcctttca cacgagcact ggcgacgccg tcgtgcccgg caccgcgggc
     6781 cgctgcgaag gaccgccgca atcgcgggtc tcgcgctgct cgccgtcgcc gcggccataa
     6841 ccggctcgct gctgctgctc gctggagacc ccggcttggc agtgggctgc aaccttgcct
     6901 cggctcatcc gcgagtggtc gggcgcgaca cgttcatccg agcggagaac ggcatggtgc
     6961 tcggcgcagt gccaaccgcg cagaaccgcg agccggtgcc gctcgaacgg atgtcgccat
     7021 ggctccccaa ggccacggta gcgatcgagg accggcggtt ctggcagcac ggcgccctcg
     7081 actacgacgc gatcctgagg gcagcgatcg cagacctcaa ggcggggcgc gtccttcagg
     7141 gcggctcgac gatcacacag cagttcgtgc gcgaccgcta cctcgcgggg cagggggcga
     7201 ccttgcatcg caagctgaag gaggcttgcc tggcggtcca gctcgcgcac gaatggccga
     7261 agcggcggat cctgcaggcc tacctcaacc ttgtgttcta cggcgggcag gcctacggtg
     7321 ccgaggcggc ggcgcgcacg ttcttcggtc gcagcgccaa cagcctcacg ctcgcccaag
     7381 cggcgctgct ggccggcctg ccgcaagctc cttcggtcta cgacccgttc gccaggccgg
     7441 tcgcggcgcg cgcccggcgc aatgaggtgc tgcgggccat ctgcaaagcc ggatacatat
     7501 cgacagcccg ctaccggtcc gcgtcgtcat cccccgtgcg cctccatccc agcgcgcgtt
     7561 acacccgcat cctcgcggcg ccgttcttca actacgtgac gcgtgagctg gtgcgtcgct
     7621 tgggcgcgca gcgggcgcgg cgcggcgggc tcacggtgga gaccacgctc gatccgcgcc
     7681 tccagcggct cgcgaacaag gcgatctccg gctggctgta caagccgtcc gatcccgctg
     7741 cggctctcgt ggcgatcgac ccgcggaacg gcgccgtgcg cgcgatgacc ttcgcgaacc
     7801 cgggccgcgc ggcgctcgcc ttcaatctcg cgagccagtc gcaccggcag gcgggcagcg
     7861 cgttcaaggt cttcaccctc acggcggcga tggagcgcgg gatcgcgttg aactcggtct
     7921 ggcacggccc cgcctcgctc acgatcccga accgaagctg cctgaacgcc aacggcccct
     7981 gggtggtcca caacttcgcg gacgaggcga cgggcacgat gaccttgctc cgggcgatcg
     8041 cgcactcggt caacacgatc ttcgcgcagg tgatcatgcg cgtcggaccc gcgaatgtgg
     8101 tcgatgttgc tcaccgcatg ggcatccgct ctcacctgaa gccggtgtgt gcgctcacgc
     8161 tcggccccga gggtgtctcc ccgctcgaga tgaccacggc tttcgcgacg ctcgccgcgc
     8221 gcggcatccg ccaccgccca caaccactga gcgcggtgct gggacgcggc ggcagagccc
     8281 tgatgcgcct cgacgcgaac ggcaagcgag cgctgtcgca gtcggtcgtc gaccgggtca
     8341 cgtacgcgct tacgggcgtg gtccgcgccg gaaccggaag ggctgccgac cccggtcgcc
     8401 cggcggcggg caagaccgga acggcggaga acttcaagga cgcgtggttc tgtggctacg
     8461 tcccgcagct cgcgacgtgc gtgtggatcg gctatcccca ggcggagatc ccgatgcacg
     8521 gcgtgggcgg gttcgcgcag gtggtgggcg gctcagtgcc cgcgcggatc tggcatgact
     8581 tcatgctccc ggcgatggcg ggacaaccgg tgcgcgggct gccgaccgcg agcccagggc
     8641 gtgtggggcc ggcaccccag gctccaccac tgccgccgct ctcgcccccg gtgcccgcga
     8701 cgataacgcc ggcgcttccg cgttgaggcg ctcagtcctg gcagatgtca gcgacgattg
     8761 ggtccgcgag gcgcgtcagg tcgttgggtt tcatccacag cgagtgggtg tggtcgcccg
     8821 cggtgcatgc gacgcgctcg cagtcgagca gccgcggatc catgacttcg agcgacggca
     8881 tcgtgggacc gacgggcggc agcgcaccca gctcgtactc gggaaactcg gccgccatct
     8941 cgtcttccga tgcgaggcgc agctcctcgc cttggcccac acgcgcgcgt agcttgtgta
     9001 gatcgaggcg ctcggaagcg ggcaccacgg cgagcccgta gcttccgccg attcgaagca
     9061 ggaccgtttt cgcctcgtcg gctgcgcgtg ccccgacggc ttgcgcgtcc tctgccgcgg
     9121 tcaccgtgcg gcggtgctcc acgacctcgt agtcgacgcc cgcggcgtcg aggtaatccg
     9181 tcagtgcggt catctccatc ccccctcttg gatgcagcga tgactctacg cgctctcggc
     9241 cccgatgcac cagcggatcg gggccgagaa acgcgatccg ttacttgatg cggagagcgc
     9301 tgaagactcc ggcgtggtcg gagtcccagt agccgttctg catctcgcgg ccggtgacgg
     9361 ccgagtccac gagctgcacc tcgtcgggcg cgtttgtgag cacgtggtcg acctggtggt
     9421 cgaactccgc gtccgtgccg gtctggaggt tcgccgagtg cacgcagcag ctgagcgggt
     9481 cgttcgtgct gcgctcggcg aagccggcgc cggtcaggac gttgtacgcc tgctggtcgc
     9541 cgttgaacac ggtgtcgtcg tccgagttga agtcgccgag cagcacgacc ggcaggtcgc
     9601 cgctcgccgg cccgcccgga gccacgagct cgcccgcctg cttggcgcgg atgctcggga
     9661 ccacgcttcg cgggtcgaac gcctcgaggt gggtgttcac gaagcggaac cagccgctgc
     9721 cgcgcacctt cgcgtcgatc gcggtccagc cgcgggtgac ggtcacgggg ttgccgagga
     9781 tcggcagcac cagcgggttc gcgaagtgac cggcctgcgg gttagcgacc ttcacgctcg
     9841 agttgtccac gcgcgcgagg atcacgtcgc gcatggtcag ccgcgcgttc agctcggcgt
     9901 tcacgatcgg gaacgggcca tcgccgctca cgttgttctc gttcgcgggc gcctcgaggt
     9961 cgaactcggg ctgaacgacg accgcttcgt acttcgtccc gtccttgttg agctcgtcca
    10021 gcagaagctg gaggaagtcg tagcggacgg tctccgcggt cttcggtccg cccaggacgg
    10081 gcgcgatcga cggcgccgcg gtgcgccaga gcgcgacctc ctggagaccg acgaggtcgg
    10141 gctcctgacc gagaatctcg gccgccagtc ccttggcacg aaccgggaag ttcgtcgccg
    10201 tcacctggcg caggatcccg ccgttggcgt tcacgaacgc ggtcaggttg ggtgccccga
    10261 tcgccggtgc caggtcggcg ccgaggaaca gattgcgcgt catcaccttg gccacgtggc
    10321 cttcggcgtt gccctcgtcc gcttgggccg cagcgggggc caggcagagc acaagagctg
    10381 caacgagtgc gagcttctta agcaagtaca tccctccttg ggtgttgcga gtccggggcg
    10441 atcgtaaccc aaatcggtga accggccgag aagcgggcgc gatcgcgccc cttccgggtc
    10501 cctgcgacga cgcatccgac aactcgaccc aatcggggga tggcgagggg ggcgaactcg
    10561 ggcgcatgat cgcttccggc agtgaagcca tgacgcagga gacgctccga cacggaagtt
    10621 gggcgggcgc tcggacgcgc ccgacgcgag ccgagataga cccggcggcg ctcgagctga
    10681 tcgagcggta cggggcgtcg ctcctgcgta ccgctcgccg ctactcgctc agcgcggagg
    10741 acgccgagga tgcctaccag cgcgggctcg agatcctgct caccaaggcg ccctccacca
    10801 gcgaggcgga cctgctgccg tggctgaaga cggtcgtgaa gcatgaggcg ttcgcgatcc
    10861 gccgccagcg ccagcgcgcg gagctgcgcg acgacgactc gtcctgggat ggcgccaccg
    10921 cctcgacaga cgagcaggct gagcgtttcg agcgcctgca gatcggtgcc gaggcgctcg
    10981 ggcggctgaa gccgcaggag acacgagcgc tgctgctgaa ggcgcaggga ctctcctacc
    11041 aggagatctg tgcggagacc gggtggacgt acacgaaggt caaccgttgc ttgaccgaag
    11101 ggcggcgagc ctttctcgac cgggtcgccg gcatcgagtc gggggatgag tgcgaccggc
    11161 tcgctcccca gctttccgcg ctcgccgatg gcgaggcgag cgccgaggac atggcgctgc
    11221 tgcgcccgca cctgcgcagt tgtctttact gtcgcggcgc gttgcgcgag taccgcgagg
    11281 cgccggcgcg cgtggccgtg ctcgctcccg ccgggctgct cacgctcgac tcgatggacg
    11341 aagccgctga gcgctcctgg tcgctgctcg gctggctcca cgaccgcttc atgctcgcgt
    11401 ggatgcgcgc gcacgaagcg gccctgtc
//
LOCUS       DASVXS010000281         5646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_837780, whole genome shotgun sequence.
ACCESSION   DASVXS010000281 DASVXS010000000
VERSION     DASVXS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837780"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /gene="dnaN"
                     /locus_tag="VF752_15875"
     CDS             <1..974
                     /gene="dnaN"
                     /locus_tag="VF752_15875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX8857072.1"
                     /translation="TDMEIGVRVKLDAQVERDGAVVLPGRLLLDVVRSLPDPKLSLEH
                     RTSEQDVEVVSGTARFHLRTLPLEDFPKLPEPGESPMQMPAGAFVDTVARVARAASRD
                     ETRPHLTGVLVSASSQELRMVATDSYRLSVKETKLDQAVQGELEANVPARTLQELARV
                     AGGSGAEQIAISALDNQVVFTLGDVVISSRLVEGRFPNYRQLLPESFEHELKVSGQEL
                     LEVVRRISLLAQKNAPLRLSFSEGTLEVSAQTPDVGEASESLPIPFKGEPLEIGFNPD
                     FLRDGLESSESDELALKLISPLRPGLLESGGGESKFVYLVMPIRLNV"
     gene            985..2097
                     /gene="recF"
                     /locus_tag="VF752_15880"
     CDS             985..2097
                     /gene="recF"
                     /locus_tag="VF752_15880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX8857073.1"
                     /translation="MDAVRVTHVALRDFRTYERAEAPLAPGLTVVTGANGAGKSNLLE
                     AVYFGCTGRSPRTTNERELVRFGQKVARVTVDLEAEDGAHRLEVGVEPGEAKLLRADG
                     ASVDSLASSPLRPLVSVFMPDRLDLVKGPPALRRAHTDQFTAAMWPARAEVKQAYGRA
                     LAQRNALVARVRAGAARAELLDTWDAELARCGIALMDVRREALEALAEPFARLALDLG
                     LPGEAVLRYRPRSKASTAEELQAELAARREDDLARGFTTHGPHRDDLDLQRDGRSLRA
                     YGSQGQQRAALLALLFAERELLAARGRSPLMLLDDVMSELDVARRERLIELLRRGGQA
                     VVAATEPEHVPGSGDPDVRLLNVVEGTIVSYDGVAA"
     gene            2151..2408
                     /locus_tag="VF752_15885"
     CDS             2151..2408
                     /locus_tag="VF752_15885"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX8857074.1"
                     /translation="MVERAAPRTTLARVQGAWESVAGPVVAAQSTPESERAGVITVRC
                     FSAVWAQELELLSGDLMASLEARLGAGAVRELRFVTGTRRA"
     gene            2613..5036
                     /gene="gyrB"
                     /locus_tag="VF752_15890"
     CDS             2613..5036
                     /gene="gyrB"
                     /locus_tag="VF752_15890"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX8857075.1"
                     /translation="MAKEAPRSNGKSSYGAQDITVLEGLEAVRKRPGMYIGSTGQRGL
                     HHLIYEVMDNSVDEALAGEADNVKITIHPDNAVTVTDNGRGIPVDTMEKEKLPAATVV
                     LTVLHAGGKFGDGGGYKVSGGLHGVGVSVVNALAEHLDLTVWRDGFEWTQSFERGKPL
                     GDLKKGSKTDRRGTRITFMPDLEIFETIDYDRTVLEQRFREMAFLTKGLNIEFDDERG
                     ERFSTSFKYDGGIKDFVAYLHSQGTKTPIGKKIVYLEDEGDVGEVEIAMQWNDSYQEN
                     LLSFANNINTHEGGSHLSGFRSALTRTLNAYAREKGLLKEKEDNLAGEDVREGLTAII
                     SVKVPEPQFEGQTKTKLGNPPVEGFVQQAVNRGLAEFLEETPTEARQVITKAVQAARA
                     REAARKARDLTRRKSALENSTLPGKLADCSVKDPALAELFIVEGDSAGGSAKQGRDRN
                     TQAVLPLRGKIINVEKNRIDKVLGNQEIQALITAIGTGIREEFDLERARYHKVIVMCD
                     ADVDGAHIRTLVLTFLFREMPELIEAGYVYLAKAPLYRLKAGQREIYIEKESELEEIL
                     LRDKLEKLEVFDRDGKQFKLTHPRWQKFGRLLKQYEGWASSLRADFGHETVTFLEESQ
                     ILDDGLMSADEVIDALGKPTPEGEAFEANLVNQDAAQLVVQAIERRTGSAATYRLSRH
                     MFETPEYASFVRVHGELKELAGTPPFSVRLGKKEDQALSFEDLRRAVLDVAKEGVALQ
                     RFKGLGEMNPDQLYDTTMDASQRTLQRVTIDDASAADQIFSMLMGDRVEPRREFIEAH
                     ARDVTNLDV"
     gene            5053..>5646
                     /locus_tag="VF752_15895"
     CDS             5053..>5646
                     /locus_tag="VF752_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8857076.1"
                     /translation="MATFDTISGGSIEDRGLEEEMRSSYLDYAMSVIVGRALPDVRDG
                     LKPVHRRVLYSMYESGLRPDRPYRKCANVVGGVMGNYHPHGDQAIYDTLVRLAQEFAQ
                     RYPLVDGQGNFGSIDNDPPAAMRYTESRLARLALEMLRDIDADTVDFGPNYDESRQEP
                     LILPARFPNLLVNGSAGIAVGMATNIPPHNLGEAIDAT"
BASE COUNT          947 a         1883 c         1966 g          850 t
ORIGIN      
        1 ccaccgatat ggagatcggt gtgcgcgtga agctcgacgc gcaggtcgag cgcgacggcg
       61 cagtcgtgct gcccggccgg ctgctgctcg acgtggtgcg ctcgctgccc gacccgaagc
      121 tctcgctcga gcaccggacc agcgagcagg atgtcgaggt ggtctcgggc accgctcgct
      181 tccacctgag gacgttgccg ctcgaggact tcccgaagct ccctgagccg ggggaatcgc
      241 cgatgcagat gccggcgggc gcgttcgtcg acaccgtcgc ccgggtcgcg cgcgcggcct
      301 cgcgcgacga gactcgccca cacctcaccg gggtgctcgt gtcggcctcg agccaggagc
      361 tgcggatggt cgccaccgac tcttatcgcc tgagcgtgaa ggagacgaag ctcgaccagg
      421 cggttcaggg tgagctcgag gcaaacgtgc cggcgcgcac gctgcaggag ctcgcacgcg
      481 tggccggcgg cagcggcgcc gagcagattg cgatctcggc gctcgacaac caggtggtgt
      541 tcacgctcgg cgacgtggtg atctcgtcgc ggctcgtgga aggtcgattc ccgaactatc
      601 gccagctgct gcccgagtcg ttcgagcacg agctgaaggt gagcggccag gagctgctcg
      661 aggtggtgcg gcgcatcagc ctgctggcgc agaagaacgc gccgctgcgg ctgtcgttca
      721 gcgaggggac gctcgaggtg tctgctcaga ccccggatgt gggtgaggcg agtgagtcgc
      781 tgcccattcc gttcaagggc gaaccgttgg agatcgggtt caacccggac ttccttcgcg
      841 acggcctcga gagctcggag tccgacgagt tggcgctcaa gctgatcagc ccgctgcgcc
      901 cgggcctgct cgaatcgggt ggcggggaaa gcaagttcgt ctacctcgtg atgccgatcc
      961 ggctcaacgt gtaggggcag ggcggtggac gcggttcgcg tcacccacgt ggccctccgt
     1021 gacttccgca cctacgagcg cgcggaggcg ccgctcgcgc cgggactgac ggtcgtgacc
     1081 ggcgcgaacg gagccggcaa gagcaacctg ctcgaggcgg tgtacttcgg ctgtaccggt
     1141 cgctccccgc gaacgacgaa cgagcgcgag ctcgtgcgct tcgggcagaa ggtggcgcgg
     1201 gtcaccgtcg acctggaagc cgaggacggc gcacaccgcc tcgaggtcgg tgtcgagccg
     1261 ggtgaggcga agctgctccg cgcggatggg gccagcgtcg actcgctggc gtcttcgcca
     1321 ctgcgcccgc tcgtcagtgt gttcatgccc gatcgcctcg acctggtgaa aggcccgccg
     1381 gcgttacgcc gcgctcatac cgaccagttc accgcggcga tgtggccggc gcgggcggag
     1441 gtgaagcagg cgtacgggcg cgcgctcgcg cagcgcaatg cgctggtcgc gcgcgtgcgt
     1501 gcgggggctg cccgcgcgga gctgctcgac acctgggacg cagagctggc tcgctgcggc
     1561 atcgcactca tggacgtgcg gcgcgaggcg ctcgaggcgc tcgcggagcc gttcgcgcgg
     1621 ctcgcgctcg acctcggcct gccgggcgag gccgtgcttc gctaccggcc gcgctcgaag
     1681 gcctcgacgg cagaggagct tcaggcggag ctcgccgccc gccgggagga cgacctcgcc
     1741 cgcggcttca ccacgcacgg gcctcaccgc gacgacctcg accttcagcg cgacggccgc
     1801 tcgctacgcg cctacggttc gcagggtcag cagcgggcgg cgcttctcgc tctgctcttc
     1861 gcggaacgcg agctcctcgc cgctcgcggc cgctcgccgc tcatgcttct cgacgatgtg
     1921 atgtccgagc tcgacgtggc ccgccgcgag cgcctgatcg agctgctgcg tcgcgggggc
     1981 caggccgttg tcgccgccac cgagcccgag cacgtgccgg gctccggcga tccggacgtg
     2041 cggctcttga acgtggtgga gggcaccatc gtcagctacg acggcgtcgc ggcgtgatgc
     2101 ggagcgcccg acggcatgca ccgcggccgc tcgcgccggc gctcgaagcc gtggtcgagc
     2161 gggctgctcc gcgcaccacg ctcgcacgcg tccagggggc ctgggaatcg gtggcgggtc
     2221 cggtcgtggc ggcccagtcg acgccggaat ccgagcgagc cggcgtcatc acggtgaggt
     2281 gtttttctgc ggtctgggca caggagctcg agcttctctc gggcgacctc atggcctccc
     2341 tcgaggcccg tctgggcgcg ggcgccgtgc gtgagcttcg cttcgtcaca ggcacccgcc
     2401 gggcgtgaga acagcctatg gataactttg ggaaaccatc cccgcgcggt tgggaccaat
     2461 ttgttgcgat ttgcggggat tttttacgct cagaagcggc ttttccgggg ccgcgcctgc
     2521 tacccttagt acaggcagat ttcaccgccc cggccactgg gcaaagctgt ccggagccgg
     2581 ggcttctcgt gtgatgaaag aagggaagcc gtttggctaa ggaagccccg cgcagcaatg
     2641 gaaagtcgag ctacggcgct caggacatca cggtcctcga gggcctcgag gccgttcgca
     2701 agcggcccgg catgtacatc ggctcgaccg gccaacgcgg cctccaccac ctgatctacg
     2761 aggtgatgga caactcggtc gacgaggcgc tcgccggcga ggccgacaac gtcaagatca
     2821 ccatccatcc ggacaacgcg gtcacggtca ccgacaacgg tcgtggcatc cccgtcgaca
     2881 cgatggagaa ggagaagctg ccggcagcta cggtcgtgct caccgtgctc cacgctggag
     2941 gcaagttcgg cgatggcggc ggctacaagg tgtccggcgg cctccacggc gtcggcgtgt
     3001 cggtcgtgaa cgcgctggcc gagcacctcg acctcacggt ctggcgcgac ggcttcgagt
     3061 ggacgcagtc cttcgagcgc ggcaagccgc tcggcgacct caagaagggc tcgaagacgg
     3121 atcggcgcgg aacgcgcatc acattcatgc ccgacctcga gatcttcgag acgatcgact
     3181 acgaccgcac tgtgctcgag cagcgcttcc gcgagatggc gttcctcacc aagggcctga
     3241 acatcgagtt cgacgacgag cgcggcgaga ggttctccac cagcttcaag tacgacggcg
     3301 gcatcaagga cttcgtcgcg tacctccact cgcagggcac gaagacgccg atcggcaaga
     3361 agatcgtcta cctcgaggac gagggcgatg tcggcgaggt cgagatcgcc atgcagtgga
     3421 acgactccta tcaggagaac ctgctctcgt tcgcgaacaa catcaacacg cacgagggcg
     3481 gctcgcacct ctccggcttc cgctcggcgc tcactcgcac gctcaacgcg tacgcgcgcg
     3541 agaaggggct gctgaaggag aaggaggaca acctcgcggg cgaggacgtg cgcgagggcc
     3601 tcaccgcgat catctccgtg aaggtccccg agcctcagtt cgagggccag acgaagacga
     3661 agctcggcaa cccgccggtc gagggcttcg tgcagcaggc ggtgaaccgc ggcctggcgg
     3721 agttcctcga ggagaccccg accgaggcgc ggcaggtgat caccaaggcc gtgcaggcag
     3781 cccgtgctcg cgaggctgcg cgcaaggcgc gcgacctcac gcggcgcaag tccgcgctcg
     3841 agaactcaac gctgccgggc aagctcgccg attgctcggt gaaggacccg gcactcgccg
     3901 agctcttcat cgtcgagggc gactcggccg gcggctccgc gaagcagggg cgcgaccgca
     3961 acacgcaggc ggttctgccg ctgcgcggca agatcatcaa cgtcgagaag aaccgcatcg
     4021 acaaggtcct cggcaaccag gagatccagg cgctgatcac cgcgatcggc accggcattc
     4081 gtgaggagtt cgacctcgag agggcccgct accacaaggt gatcgtcatg tgcgacgccg
     4141 acgtggacgg cgcgcacatc cgcacgctcg tgctcacgtt cctcttccgg gagatgccgg
     4201 agctgatcga ggccggctac gtgtatctgg cgaaggcacc gctgtaccga ctgaaggccg
     4261 ggcagcgcga gatctacatc gagaaggagt cggagctcga ggagatcctg ctccgcgaca
     4321 agctcgagaa gctcgaggtc ttcgaccgcg acggcaagca gttcaagctc acccacccgc
     4381 gctggcagaa gttcggccgg ctgctcaagc agtacgaggg ctgggcgtcg tcgctgcgcg
     4441 ccgacttcgg gcacgagacg gtcaccttcc tcgaggagtc gcagattctc gacgacggtc
     4501 tcatgtcggc cgacgaggtc atcgacgcgc tcgggaagcc gacgcccgag ggtgaggcgt
     4561 tcgaagccaa cctggtgaac caggacgcgg cgcagctcgt ggtccaggcg atcgagcggc
     4621 gcaccggctc agccgcgacc taccggctca gccgccacat gttcgagacg cccgagtacg
     4681 cgagcttcgt ccgcgttcac ggcgagctga aggagctggc gggcactccg cccttcagcg
     4741 tgcggctggg caagaaggag gaccaggcgc tgtccttcga ggacctgcgg cgggcggtgc
     4801 tcgatgtggc gaaggagggc gtcgcgctgc agcgcttcaa gggcctgggc gagatgaacc
     4861 ccgatcagct ctacgacacg acgatggacg cgagccagcg cacgctccag cgagtcacga
     4921 tcgacgacgc ttccgcggcg gaccagatct tctccatgct catgggcgac agggtcgagc
     4981 caaggcgtga gttcatcgaa gcccacgcac gcgacgtcac gaatctcgat gtgtaaggaa
     5041 gtgatggcct aaatggcgac gttcgacacc atctccggcg gctcgatcga ggatcgcggc
     5101 ctagaggagg agatgcgatc gtcctacctc gattacgcca tgagcgtgat cgtgggacgg
     5161 gccctgcctg acgtccgcga cggcctgaag ccggtccacc gacgggttct ctactcgatg
     5221 tacgagtcgg gcctgcggcc cgaccggccg taccggaagt gcgcgaacgt cgtcggcggc
     5281 gtgatgggca actatcaccc gcacggcgac caggcgatct acgacacgct cgtgcgcctc
     5341 gcgcaggagt tcgcgcagcg ctacccgctc gtcgacggcc agggcaactt cggctcgatc
     5401 gacaacgacc cgccggctgc catgcgctac accgagtcgc gcctcgcgcg cctcgcgctg
     5461 gagatgctgc gcgatatcga cgccgacacc gtcgacttcg ggccgaacta cgacgaatcg
     5521 cggcaggagc cgctgatcct ccccgcgcgc ttcccgaacc tgctcgtgaa cgggtcggcg
     5581 gggatcgcgg tcgggatggc gaccaacatc ccgccgcaca acctcggcga ggcgatcgac
     5641 gccacg
//
LOCUS       DASVXS010000282        12370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_694482, whole genome shotgun sequence.
ACCESSION   DASVXS010000282 DASVXS010000000
VERSION     DASVXS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694482"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VF752_15900"
     CDS             complement(<1..1236)
                     /locus_tag="VF752_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HEX8857077.1"
                     /translation="MEALGQAEAGDARAARDRSGGALVFVGFMGAGKTSAARTVAGEL
                     GVEHHDLDRELERDLGESIESFFDREGEPAFRAREEEVAVRMLANPAVRVASLGGGSL
                     GSARVREALANHVVVLLEVDARDAWQRASGKGRPLARDRARFEQLLADRLPLYESVAD
                     AVLPAADRGLPRRALPGLDALRQAPPRTRMVWAAASSGEYPVFAGHGLVASGFFHPRD
                     GRRFVVTDENLARHHRVPSESEIVIPAGETEKTLARVEEVLRALATAGAERGDIVVGV
                     GGGVVGDLSGFAAAIYQRGVRHVQVPTTLVSQVDSAYGGKTGVDLPEGKNYAGAFHQP
                     SAVIVDRDALRTLPREELAAGYAEVVKTALIAGGQLWARVRGGAAPDDDEIVLGCIRT
                     KLAVVAEDERDAGRRQVLNL"
     gene            complement(1224..2387)
                     /gene="aroC"
                     /locus_tag="VF752_15905"
     CDS             complement(1224..2387)
                     /gene="aroC"
                     /locus_tag="VF752_15905"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX8857078.1"
                     /translation="MTIRFTTAGESHGPGLTAVVEGLPAGLELAPEDIDRDLARRQLG
                     HGRGGRMKIERDRAQVTSGVRHGRTLGSPVALRIDNLDYANWEERMNPWPVEAELAEV
                     HLPRPGHADLAGIQKYGFTDVRNVLERASARETAARVAAGALAKVYLRAFGVEVRSHV
                     LQIGSVCGPEDGELTVESFEAVDESPVRSLDADASQAMVEEIDRARKANESLGGVFEV
                     IAFGLLPGIGSHISWETRLDGRLGHAIMSIQAMKGCSIGDGFEVAGRVGSQAHDEIFW
                     SEERGFYRETNRAGGLEGGMTTGEPLVVRGAMKPLPTLTKPLRSVDTETHEPAQALRE
                     RTDSCTVPAAGVVGEAMVALVLADAYRDKFGGDHIDDALAAHRAYEERIGWRR"
     gene            2457..2738
                     /locus_tag="VF752_15910"
     CDS             2457..2738
                     /locus_tag="VF752_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857079.1"
                     /translation="MLRVVGTWTGRVLLMLAGLTCGALAIGFAVVTVLDAMGKFEGPS
                     DEVPGLPPIPVITAPLALFCAAFAFGCGWAFYDSFRSESEQPEALYPPL"
     gene            complement(2745..3674)
                     /locus_tag="VF752_15915"
     CDS             complement(2745..3674)
                     /locus_tag="VF752_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857080.1"
                     /translation="MKKLALEVWHDLREKRLWPVAAVLALALVAVPVVLKKNSSAAGP
                     ATPTAAAPKKLGPVVKPADLVAQNSKLGVFASAKDPFTPTGKAGRELLAAAKAQSNSG
                     SNSTSSNTPSTPSGGSDNGSSGGTAPPADTPDTGNGGGGGDGSQPSEPTKPKGAYTYT
                     VDVKVGVRGSEKTHKGVARLDVLPSDRNPLIVFLGVTSSRDTAVFLLDNTLQADGEGR
                     CEPSGDVCSFLYLKVDRDHNKEEFVKTDEDGTQTEYSIQLLKINRVPASQAKASAKRS
                     AAAKRSAKAYAKKGKPTPFRFLFDVPAFADEVG"
     gene            complement(3671..4633)
                     /locus_tag="VF752_15920"
     CDS             complement(3671..4633)
                     /locus_tag="VF752_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857081.1"
                     /translation="MSMSSRDRKLILIIVPIVLLAAYWMLMLKPKRDEAAKLGKELTE
                     QRQTRDDAVAQANTLGSMKDSFATDYVTLVRLGKAIPENVDMPSLLLQLDRAARGTHI
                     DFGKIQVGERAAAAAASGATPAPASTTPGAGNGSQPASAGGAQAQSAPGKTSENAANN
                     VNNANANIAQNQAANQAATNGQPAPAAGAAPAGGGAARTSSAPGLDTVPLDFTFTGRF
                     FDLADFFHRLKRFVYVANNSISVQGRLMTIDNLQFATDQNDPKKLAATVHATVYLAPK
                     AEGTTAGATPNGPAPAPAAGAPNAQPASGSTPAPSSPPAATVTP"
     gene            complement(4633..5352)
                     /locus_tag="VF752_15925"
     CDS             complement(4633..5352)
                     /locus_tag="VF752_15925"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX8857082.1"
                     /translation="MRPVNLLPASHRPYVASGEHKGSAYVLLGVLGVLLVGALLYVLT
                     ANQVSSRQADIAKAKAETASAQSRASTMANYGDFGTIAKTRVDSVKSLAQQRLDYERL
                     VREIAHVLPAGVWVTQFTASADGSDSSASGGTPPAAGGEPSSTPAGPSVKISGCSDDQ
                     PAVATTLVRLRSLHGATDVQLQQSTKQDTPGGGSASGGSTSTQGCGTAYAFDAVVTLA
                     PATDASADKPGSVPARLGGGS"
     gene            complement(5349..6287)
                     /gene="pilM"
                     /locus_tag="VF752_15930"
     CDS             complement(5349..6287)
                     /gene="pilM"
                     /locus_tag="VF752_15930"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX8857083.1"
                     /translation="MRLPLPKKSQSGTVGLDLDGRFLSAVQAGDRKVARAVSSELPAG
                     LIKDGEVVDIDGLSEAIKGFFKEAGLPNRVRLGVSNQQIVVRHLELPQIANPTELAAA
                     VRFQAAEAIAMPLDEAVLDYQVLGETTAPDGSARLRVLVVAARAAMIERFVDSVSSAG
                     LKAEGIDLNAFALVRALSKDASEPAEDTPATVYCHLGGVTNLAIAVGSSCLFTRPLST
                     DWDEDGEHVAFALAEEIRLSIDYYMAQPDARPAGEVKLSGPGARIEGLAEQLEPLIHL
                     PVSVAEPLGALTHGELGDEDPYRHTVAAGLALGAAA"
     gene            complement(6373..7134)
                     /locus_tag="VF752_15935"
     CDS             complement(6373..7134)
                     /locus_tag="VF752_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8857084.1"
                     /translation="MGALAVIPGLFGAVVGSFLNVVIHRLPRGESIVKPRSRCGSCGE
                     QIAALDNIPLFSWALLRGRCRNCGERISVRYPVVELLTAVLFAAVVVVRGVHLELLIE
                     LPFAAMLIAVAVIDFDHKIVPNKVLLPAAVYGLVASAAIRPEKLPELLIAGAGAFTFL
                     LVAALIHPKGMGMGDVKLLGVMGLYLGRYVIPAGAVGFLVGTVAGIAILARYGMQARK
                     MKVPFGPFLALGGLVALLAGPDLVDLYRDAFLGQG"
     gene            complement(7220..8536)
                     /locus_tag="VF752_15940"
     CDS             complement(7220..8536)
                     /locus_tag="VF752_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857085.1"
                     /translation="MSRLRKQEGSVLVIAILVTAMMTMVGLAVFSTVDTQQRESGTER
                     VRESSLNLNEAVIYAQGFAMASSNQWPGNANAAMPFSCTSAAVTVPKCPDRDTLAKAS
                     SANPSLANFATTDFNAGSTWVTKVRDNYGELAGSYIPSQADGDLHGVSGTCAAPCSWD
                     FNGDRQLWVQATTMVRGRPRSIVARLQRELLTETIPQAAVTAGHFDVTNNGKHGGSYL
                     IDGSGASIGVRCDPNPASPTYDPNCATYRDGQVAPLPTQLPDSASPKPLMTAGQLSRL
                     RDRAIQDGKYYPGCPPDDIRGSVVWVENCPYQKFTASTGSNPCPATLPSGFSQQKCIN
                     TDQSPGVLIWHHGTLEISGNVTYIGLVYAVNGSDDGVDQSTGTVVTTQGGGGIWGSVA
                     IDGRGGLDAGSNGFNVKYNANIFPNIKSYGTAGLVQNTWRELPPGS"
     gene            complement(8536..9477)
                     /locus_tag="VF752_15945"
     CDS             complement(8536..9477)
                     /locus_tag="VF752_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857086.1"
                     /translation="MIELLVVMPLFIVVLFATLGVFDQFGQNAATNRKQTDAIDQSRF
                     TIDTLTRQLRNLANPTSAGSTINTAEPYNFVFQTSDPSRTWVRYCLAQPSGSQAGKVW
                     FASTTAATVSSGMTSSSSCPGTGWEVTRVVAQDVTNQYSGSATPLDRPLFTYRCSGGG
                     SVCPASPADFPRILNATANVYVDVNPGKKPAERRITSGVYLRNQNEAPHAQFTVGYGP
                     QLSRTVVLNASASDDPEGRTLKYYWYKGTGTQPTAPGDSCPAVSGTATYLGEGVTFTY
                     KFPDSDGPSGTPNQTIQLVVKDPGCLSDDADQAVKIP"
     gene            complement(9510..11021)
                     /locus_tag="VF752_15950"
     CDS             complement(9510..11021)
                     /locus_tag="VF752_15950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8857087.1"
                     /translation="MSLHSLRSDERGFTLVELMAAIIVLLAGVLGAVALIDGANAGTT
                     TASDRVGAVNLARELTEGARAADYNKLDPANIVDELRNQPQLAGSYSGSWTLNRRGVT
                     YTVTTDVCTYDDKKDGIATTPPANVCANEPTSSTTDSNGDDFRRITFTVAWILNTKTR
                     SVQQTTLIPNPSGGLGPRVVSLSETLVAIPPGDAGTTCPSTPPSASVSINAVTTPAAS
                     LHWTTDNAGSEGDATSADGRNWPINWNLDSPLVLDGTYTLTAQAFDDRGIPGDVKAAT
                     VALNRSCPQQPSGFQGGFNHRSTTGHDNGVLEFQWDANQERDIVGYRVYWAGPDGAQG
                     GGNDVQICPASGTTPIPTTSCYADRPAGAGTSATYYVVAVDLRDIMNPQSPPRQGDPR
                     MLAMTDPGDPRPTWPDGTTLTATLDGGYPTLTWDQAASPATGHTIRFYRIYRDGDPSS
                     LSNRNNWVGFSSSTPPFVWPDPSPGSTTSHQYWVTAVDDQFNESDPVGPVTSP"
     gene            complement(11160..11600)
                     /locus_tag="VF752_15955"
     CDS             complement(11160..11600)
                     /locus_tag="VF752_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8857088.1"
                     /translation="MLRTLRERIQTEESGFTLIELLVVILIIGILAAIALPTFLGQQK
                     KGQDASAKSDARNTVSAMESCYTDTEDYTQCDSAKLSSLGTPVQNSVTITPNGASAYA
                     VTATSKSGNTFTITKATTGTFSRDCTTHGQGSCKSAADSNGNYW"
     gene            complement(11792..12232)
                     /locus_tag="VF752_15960"
     CDS             complement(11792..12232)
                     /locus_tag="VF752_15960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX8857089.1"
                     /translation="MLRTLRMRIQSEESGFTLIELLVVILIIGILAAIALPTFLGQQK
                     KVQDASAKSDARNTVSAMESCYTDTEDYTQCDKAKLSALGTPVQNSVTITPNGASAYA
                     VSATSKSGNTFTITKATTGTFSRDCTTHGQGSCKSAADSNGNYW"
     regulatory      complement(12289..12365)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1801 a         4161 c         4358 g         2050 t
ORIGIN      
        1 gaggttcagc acctgccgcc ggcccgcatc gcgttcgtcc tcggcgacca ccgcaagttt
       61 ggtccggatg cagccgagca cgatctcgtc gtcgtcgggc gcagcgccgc cgcggacgcg
      121 cgcccacagc tgcccgccgg ctatgagggc cgtcttcacg acctccgcat agcccgcggc
      181 gagctcctcc cgcggcaacg tccgcagcgc gtcgcggtcg acgatcacgg cgctcggctg
      241 gtggaacgcg cccgcgtagt tctttccctc gggcaggtcg acccccgtct tgccgccgta
      301 ggccgagtcc acctgcgaca cgagcgtggt cggcacctgc acgtgtcgca cgccgcgctg
      361 gtagatcgcc gcggcgaagc ccgagaggtc ccccacaacg ccgcctccga cgccgacgac
      421 gatgtcgccg cgctcggcgc ctgcggtggc caacgcgcgc agcacctcct ctacgcgcgc
      481 gagcgtcttc tccgtctcac cggcgggaat gacgatctcc gactcactcg gcacgcggtg
      541 gtggcgcgcg aggttctcgt ccgtaaccac gaagcggcgg ccatcgcgcg ggtggaagaa
      601 gcccgaggcg accaagccat gcccagcgaa gacgggatac tcgccggacg aggcggccgc
      661 ccacaccatg cgggtacgag gcggcgcctg gcgcagcgcg tcgaggccgg gcagcgctcg
      721 ccgcggcagt cctctgtcag ctgccggcag caccgcgtcc gcgactgact cgtacagggg
      781 cagcctgtcc gcgagcagct gctcgaagcg cgcgcggtcg cgtgcgagcg gccggccctt
      841 gccggaggcg cgctgccatg cgtcgcgggc gtcgacctcg agcagcacga ccacgtggtt
      901 ggcgagcgcc tcgcgcacgc gcgcgctgcc gagcgagccg ccaccgagcg aggcgactcg
      961 cacggccggg ttggcgagca tgcgcaccgc cacttcttct tcacgcgcgc ggaaggccgg
     1021 ctcgccctcg cggtcgaaga aggattcgat cgactcccct aggtcgcgct cgagctcacg
     1081 gtcgaggtcg tgatgctcaa cgccgagctc cccggcgacc gtccgggcgg cgctcgtctt
     1141 gcccgcgccc atgaacccga cgaacacgag cgcgccgccg ctgcggtcgc gagccgccct
     1201 tgcgtcgccg gcttcggctt ggcctagcgc ctccatccga tccgctcctc gtaggcgcgg
     1261 tgcgccgcca gggcgtcgtc gatgtgatcg cccccgaact tgtcgcggta cgcgtcggca
     1321 agcacgagcg ccaccatcgc ctcccccact acgcccgcgg ccggcaccgt gcaggagtcg
     1381 gtgcgctcac gcagcgcctg ggcgggctcg tgggtctcgg tgtcgaccga gcgcaggggc
     1441 ttcgtgagcg taggaagcgg cttcatcgcg ccgcgcacca cgagcggctc gcccgtcgtc
     1501 atcccgccct cgaggccgcc ggcgcggttg gtctcgcggt agaagccacg ctcctccgac
     1561 cagaagatct cgtcgtgggc ctgcgaaccc acgcgacccg ctacctcgaa gccgtccccg
     1621 atcgagcagc ccttcatcgc ctggatcgac atgatcgcgt gaccgaggcg cccgtcgagc
     1681 cgtgtctccc aggagatgtg cgagccgata cccggcaaga ggccgaacgc gatcacctcg
     1741 aacacgccgc cgagggactc gttcgccttt cgggcgcgat cgatctcctc gaccatcgcc
     1801 tgcgacgcgt cggcgtcgag ggagcgcacg ggcgattcat ccacggcctc gaacgactcc
     1861 accgtaagct cgccgtcttc gggcccgcag accgagccga tctggagcac gtggctacgc
     1921 acctccaccc caaacgctcg gaggtagacc ttggcgagcg cccccgcggc gacccgcgcg
     1981 gcggtctcgc gcgcgctcgc acgctcgagc acgttgcgga cgtcggtgaa gccgtacttc
     2041 tggatgccgg cgaggtcggc gtgtcccggg cgcggcaggt gcacttcggc gagctccgcc
     2101 tcgaccggcc acgggttcat ccgctcctcc cagttggcgt agtcgaggtt gtcgatccgg
     2161 agcgcgaccg ggcttccgag cgtgcgcccg tggcgcacgc cgctcgtcac ctgagcgcgg
     2221 tcgcgctcga tcttcatgcg gccgccgcgc ccgtgcccga gctggcgcct cgcgaggtcg
     2281 cgatcgatgt cctctggggc gagctcgagg cccgcgggca ggccctcgac caccgccgtg
     2341 agcccggggc catgggactc cccggcggtc gtgaagcgga tcgtcatcgg gctcttaccc
     2401 tacttcgacg ccgtcccgga ttgcgcctga ccgaggcgcg cgctaccttc gctcgcgtgt
     2461 tgcgagtagt cggcacatgg acaggtcgcg tgctgctaat gctcgccggc ctcacctgcg
     2521 gagcgctggc gatcggcttc gcggtggtga ccgtgctcga cgcgatgggc aagttcgagg
     2581 ggccgagcga cgaggtgccg ggcctgccgc cgatcccggt gatcaccgcc ccgctggctc
     2641 tgttctgcgc ggcgttcgcg ttcggctgcg gctgggcgtt ctacgactcg ttcaggtcgg
     2701 agagcgagca gccggaggcg ctgtacccgc cgctgtaagg cccgctagcc gacttcgtcg
     2761 gcaaatgccg gcacgtcgaa gaggaagcgg aacggcgtcg gcttgccctt cttcgcgtag
     2821 gccttcgcgg agcgcttcgc ggccgcgctg cgcttcgccg aggccttcgc ctgcgacgcc
     2881 ggcacgcggt tgatcttcag aagctggatc gagtactcgg tctgcgtgcc gtcctcgtcc
     2941 gtcttcacga actcttcctt gttgtggtcg cggtcgacct tcaggtagag gaacgagcag
     3001 acgtcgcctg agggctcgca gcggccctcg ccgtcggcct gcagcgtgtt gtcgagcagg
     3061 aagaccgcgg tatcgcgcga cgacgtcacg ccgaggaaca cgatcagcgg gttgcggtcg
     3121 ctcggcagca cgtcgagccg cgcgacgccc ttgtgcgtct tctccgagcc gcgcacaccg
     3181 accttcacgt cgaccgtgta cgtgtaggcg cccttgggct tcgtgggctc gctgggctgc
     3241 gagccgtcac cgccgcctcc accgtttccg gtgtcgggcg tgtcggccgg cggagcggtg
     3301 ccgccactcg agccgttgtc gctaccgccc gagggcgtgc tgggcgtgtt cgacgaggtc
     3361 gaattggaac ctgagttgct ctgcgccttc gcagcggcca gcagctcacg gccggccttg
     3421 cccgtcggcg tgaacgggtc cttcgccgac gcgaacacgc cgagcttgga gttctgcgcg
     3481 accaggtcgg ccggcttcac caccgggccg agcttcttcg gcgcggccgc cgtgggcgtg
     3541 gcgggcccgg cagcagagga gttcttcttg aggacgaccg gcacggccac gagcgccagc
     3601 gcgagcacgg cggccacggg ccacaggcgc ttctcacgca ggtcgtgcca gacctctagg
     3661 gcgagcttct tcatggagtc accgtggctg ccgggggcga tgagggggcg ggggtcgagc
     3721 ccgacgccgg ctgggcgttg ggcgcgccgg cggccggcgc cggggcgggg ccgttcgggg
     3781 tggcgcccgc cgtcgtgccc tcggccttgg gtgcgagata aacggttgcg tgcacggtcg
     3841 ccgcgagctt cttcggatcg ttctgatccg tggcgaactg caggttgtcg atcgtcatca
     3901 gacgcccctg gacgctgatg ctgttgttcg cgacgtagac gaagcgcttc agccggtgga
     3961 agaaatccgc caggtcgaag aagcgaccgg tgaaggtgaa gtcgagcgga acagtgtcca
     4021 gtccgggagc gctcgaggta cgcgccgcac cgccgcccgc gggggccgct ccagcagcgg
     4081 gtgccggctg cccgttcgtg gcggcctggt tggctgcctg attctgggcg atgttcgcgt
     4141 tcgcgttgtt gacgttgttc gccgcgttct cggacgtctt gcccggggcg ctctgcgcct
     4201 gtgcgccgcc ggccgatgcc ggctggctgc cgttgccggc gccgggggtc gtgctggcgg
     4261 gagcgggtgt ggcgccgctg gctgccgcgg cggccgcgcg ctcgccaacc tggatcttcc
     4321 cgaagtcgat gtgcgtcccc cgcgcggcgc ggtcgagctg cagcagcagg ctcggcatgt
     4381 ccacgttctc gggaatcgcc ttgccgagac gcacgagcgt cacgtagtcg gtcgcgaagc
     4441 tgtccttcat cgagccgagc gtgttggcct gggcgacggc gtcgtcgcgc gtctggcgct
     4501 gctcggtgag ctccttgccg agcttcgctg cctcgtcgcg cttgggcttg agcatcagca
     4561 tccaatacgc cgcgagcaga acgatcggca cgatgatcag aatgagcttg cggtcgcggc
     4621 tgctcatgct catcacgagc cacctcccag gcgcgcaggg acgctgcccg gcttgtcagc
     4681 cgatgcgtca gtcgcggggg cgagcgtcac gaccgcgtcg aacgcgtagg cggtgccgca
     4741 gccctgcgtg ctggtcgacc cgccgctggc ggagccgccg cccggcgtgt cctgcttggt
     4801 cgactgctgc aactgcacat cggtggcgcc atgcagcgag cgcaggcgca caagcgtcgt
     4861 ggcgacggcg ggctggtcgt cggagcagcc cgagatcttc acgctcggac ctgccggcgt
     4921 cgagctgggc tcgccgcccg cggcgggagg cgtgccaccg gaggcggagc tgtcgctgcc
     4981 gtcggcgctc gcggtgaact gcgtcaccca cacgccggcg ggaagcacgt gcgcgatctc
     5041 gcgcacgagc cgctcgtagt cgaggcgctg ctgcgcgagc gacttgaccg aatcaacgcg
     5101 cgtcttcgcg atcgtgccga agtcgccgta gttcgccatc gtgctcgcgc ggctctgcgc
     5161 gctggcggtc tcggccttcg ccttggcgat gtcggcctgc cgcgagctga cctgattcgc
     5221 ggtgagcacg tagaggagcg cgcccacgag cagcacgccg agcacgccga gcagcacgta
     5281 ggcgctgccc ttgtgctcgc cgctcgcgac gtagggacga tggctcgccg gaagaagatt
     5341 gactggcctc atgccgcggc tccaagagca aggccggccg ccacggtgtg ccggtaggga
     5401 tcctcgtcgc cgagctcgcc gtgcgtgagc gcaccaagcg gttcggccac ggagacagga
     5461 aggtggatga gcggctcgag ctgctccgcg aggccttcga tgcgcgcgcc cggaccggac
     5521 agcttgacct cgccggcggg acgcgcgtcg ggctgcgcca tgtagtagtc gatggagagc
     5581 ctgatctcct cggccagggc gaaggcgacg tgctcgccgt cctcgtccca gtcggtcgac
     5641 agcgggcgcg tgaagaggca ggaggagccg accgcgatag cgaggttcgt gacaccgccg
     5701 agatggcagt acacggtggc gggcgtgtcc tcggccggct cggaggcgtc cttcgacagc
     5761 gcgcgcacga gcgcgaacgc gttgaggtcg atgccctccg ccttcagtcc ggcggacgag
     5821 acgctgtcca cgaagcgctc gatcatcgcg gcgcgcgcgg ccacgacaag cacgcggagg
     5881 cgagctgagc cgtccggcgc ggtcgtctca ccgagcacct ggtagtcgag caccgcctcg
     5941 tcgagcggca tcgcgatcgc ctcggcggcc tggaagcgca cggcggcggc gagctcggtc
     6001 gggttggcga tctggggaag ctcgaggtgg cggacgacga tctgctggtt cgagacgccg
     6061 agacgcacac ggttcggcag gccggcctcc ttgaagaagc ccttgattgc ctccgacagg
     6121 ccatcgatgt cgacgacctc gccgtccttg atcaggccgg cgggtagctc gctcgacacc
     6181 gcgcgggcga ccttgcggtc accggcctgg acggcggaga ggaagcggcc gtcgaggtcg
     6241 agaccgaccg tgccgctctg gcttttcttg gggagaggga ggcgcatgga gggagatacc
     6301 ggacctgggc tccatatcga ccgcgcgcgt gcggtccttt agccctgact cagcggctag
     6361 acgggctggt cctcacccct gcccgaggaa cgcgtcgcgg tacaggtcaa cgaggtcagg
     6421 gccggccagc aacgcgacga ggccaccgag cgcaaggaac ggcccgaacg gcaccttcat
     6481 cttccgcgcc tgcatgccgt agcgcgcgag aatcgcgatc ccggccaccg tgccgacaag
     6541 gaagccgacg gctccggcgg gaatcacgta gcggccgaga tacaagccca tcacgccgag
     6601 cagcttcacg tcgcccatgc ccatgccctt tggatggatg agcgcggcca cgagcaggaa
     6661 ggtgaacgcg ccggcgcccg cgatcagcag ctcgggcagc ttctcggggc gaatggcggc
     6721 gctggcgacg aggccgtaca cggcggccgg cagcagcacc ttgttcggca cgatcttgtg
     6781 gtcgaagtcg atcaccgcca ccgcgatcag catcgccgcg aagggcagct cgatcaggag
     6841 ctcgaggtgg acgccgcgca cgaccacgac cgcggcgaat aagaccgcgg tgagcagctc
     6901 caccaccggg tagcgcaccg agatgcgctc gccgcaattc cggcagcggc cacggagcag
     6961 cgcccacgag aagagcggga tgttgtcgag cgcggcgatc tgctcaccgc agctgccgca
     7021 acgcgagcgc ggcttcacga tggactcgcc gcgcggaagg cggtgaatga ctacgttgag
     7081 gaagctgccg acgactgcgc cgaacagccc ggggatgacg gctagtgcgc ccacgggatc
     7141 acctgcttga cggaacctgg agggtcatgg agggaactcc ccaccctttt cggcagcagg
     7201 cgccccgagc gttagccggt cagcttcccg gcggcagctc gcgccaggtg ttctgaacga
     7261 ggccggcggt gccgtaggac ttgatgttgg ggaaaatgtt cgcgttgtac ttcacgttga
     7321 agccgttcga gccggcgtcg agaccgccac ggccatcgat ggctaccgag ccccagatgc
     7381 cgccgccgcc ctgcgtcgtc acgaccgtgc cggtgctctg gtcgaccccg tcgtccgatc
     7441 cgtttaccgc gtagacgagg ccgatatagg tcacgttgcc gctgatctcg agcgtcccgt
     7501 ggtgccagat cagcacgccg ggcgactggt cggtgttgat gcacttctgc tggctgaacc
     7561 ccgacggcag cgtcgccggg cacgggttcg accccgtgct cgcggtgaac ttctggtacg
     7621 ggcagttctc cacccacaca accgatccgc ggatgtcgtc cggcgggcag cctgggtagt
     7681 acttgccgtc ctggatcgcg cggtcgcgca ggcgcgagag ctggccggcc gtcatgagcg
     7741 gcttcggact ggccgagtcc gggagctgag tcggcagcgg cgcgacctgg ccgtcgcggt
     7801 acgtcgcaca gttcgggtca taggtggggc tcgccgggtt ggggtcgcag cgaacgccga
     7861 tgctcgcgcc gctcccgtcg atcaggtaag agccgccgtg cttgccgttg ttcgtcacgt
     7921 cgaagtggcc cgccgtgacc gcggcctgcg ggatcgtctc ggtgagaagc tcgcgctgca
     7981 ggcgcgcgac gatggagcgc ggacgaccgc gcaccatcgt ggtcgcctgc acccacagct
     8041 gacggtcgcc gttgaagtcc cagctgcagg gcgccgcgca ggtgcccgag acaccgtgca
     8101 ggtcgccgtc tgcctgcgag gggatgtacg agcccgcgag ttcgccgtag ttgtcgcgca
     8161 cctttgtgac ccacgtcgaa ccggcgttga agtccgtggt cgcaaagttc gcgagcgaag
     8221 ggttagcgga gctcgctttc gcgagcgtgt cccggtccgg acacttcgga accgtaaccg
     8281 cggcggacgt gcacgagaac ggcatggccg cgtttgcgtt tcccggccac tggttgctcg
     8341 aagccatcgc gaagccctgg gcgtagatga ccgcctcgtt gaggttgagc gacgattcgc
     8401 gcacgcgctc cgtccccgac tcgcgctgct gcgtatcgac cgtcgagaaa accgcaaggc
     8461 cgaccatcgt catcatcgcg gtcacgagaa tcgcgataac gagcacggaa ccttcctgct
     8521 tgcgcaagcg agacatcacg ggatcttcac cgcctggtct gcgtcgtccg agaggcagcc
     8581 cgggtccttg accacgagct ggatcgtctg gttgggagtg cccgacgggc cgtccgagtc
     8641 ggggaacttg tacgtgaagg tcacgccctc gccgaggtac gtggctgtgc cggacacggc
     8701 ggggcacgag tcaccgggcg cggtcggctg tgtgccggtt cccttgtacc agtagtactt
     8761 gagcgtgcga ccttcgggat cgtccgaggc agacgcgttg agcacgaccg tgcggctcag
     8821 ctgcgggccg tagccgactg tgaactgcgc gtgcggcgcc tcgttctgat tgcgcaggta
     8881 tacgcccgac gtgatccgtc gttccgccgg cttcttcccg gggttgacgt ccacgtacac
     8941 gttcgccgtg gcgttgagga tgcgcgggaa gtccgccggg cttgccgggc acacggatcc
     9001 gccgccggag cacctgtaag tgaacagcgg gcggtcaagc ggtgtcgcgc tgccgctgta
     9061 ctggttcgtc acgtcctgcg ccacgacgcg cgtgacctcc cagcccgtgc cggggcagct
     9121 gctcgagctc gtcatgccgg agctgaccgt cgctgcggtc gtcgaagcga accagacctt
     9181 gccggcctgg ctgccgctcg gctgcgccag gcagtagcgc acccacgtgc gcgacgggtc
     9241 cgaggtctgg aaaacgaagt tgtacggctc tgccgtgttg atcgtggagc cggcgctcgt
     9301 cggattcgcg aggttgcgga gctgccgcgt gagcgtgtcg atcgtgaagc gcgactggtc
     9361 gatcgcgtcg gtctgctttc ggttcgtcgc ggcgttctga ccgaactggt cgaacactcc
     9421 gagcgtcgcg aagagcacca cgatgaacag cggcatcacg accaggagct cgatcaaggt
     9481 gaaccccgcc tcgtctgaac gcaacctggt cacgggctcg tcaccggtcc gacggggtcg
     9541 gactcgttga actgatcgtc gaccgccgtg acccagtact ggtgcgaggt cgtgctcccc
     9601 gggctcggat ccggccatac gaacggcggc gttgagctag agaagcccac ccagttgttc
     9661 cggttcgata gcgagctcgg atcgccgtcg cggtagatcc ggtagaaccg aatggtgtga
     9721 cccgtcgccg ggctggccgc ctggtcccag gtgagcgtgg ggtagccgcc gtcgagcgtg
     9781 gcggtcaggg tggtgccgtc gggccaggtc ggccgcggat cgcctggatc ggtcatcgcc
     9841 agcatccgcg ggtccccctg acgtggcggg gactgggggt tcatgatgtc gcgcaggtca
     9901 acggcgacga cgtagtacgt cgcactcgtg cccgcgccgg cgggacggtc cgcgtaacac
     9961 gacgtcgttg ggatcggagt cgtgccggac gcggggcaga tctgaacgtc gttgccgccg
    10021 ccctgcgcgc cgtcgggacc tgcccagtac acgcggtagc cgacgatgtc gcgctcctgg
    10081 ttcgcgtccc actggaactc gagcacgcca ttgtcatgac ccgtcgtgga gcgatggttg
    10141 aagccgcctt ggaacccact cggctgctgt gggcaactcc ggttcagcgc gaccgtcgcc
    10201 gccttcacgt cgcccggaat gcctcggtcg tcgaacgcct gtgctgtcag ggtgtaagtc
    10261 ccgtctagta cgagcgggct gtccaggttc cagttgatcg gccaattccg gccatcggcg
    10321 gaagtggcat cgccctcgga acctgcgttg tccgtcgtcc aatgaagcga cgccgcggga
    10381 gtcgtgaccg cgttgatcga gaccgaggca gacggtggcg tgctcggaca cgtggtgccc
    10441 gcatcgccgg gcggtatggc gacgagtgtc tcgctgaggc tcacgacccg cggacccagt
    10501 ccaccggacg ggttcgggat cagcgtggtt tgctgcaccg aacgcgtctt ggtgttcagg
    10561 atccaagcga cggtgaaggt gatacgccgg aagtcgtcgc cgttgctgtc ggtcgtgctg
    10621 ctggtgggct cgttggcaca gacgttcgcc ggcggcgtcg tcgcgatgcc gtccttcttg
    10681 tcgtcatatg tgcagacgtc cgtcgtgacg gtgtaggtca cgccacggcg attcagggtc
    10741 cagctgccgc tgtagctgcc ggcgagctgc ggctgattgc gcagctcgtc cacgatgttc
    10801 gccgggtcga gcttgttgta atccgcggcg cgcgcaccct cggtcagctc gcgcgccagg
    10861 ttcacagcgc ccacgcggtc gctggcggtc gtcgtgccgg cgttggcccc gtcgatcagc
    10921 gcaaccgcgc cgagcacgcc ggcgagcagc acgatgattg cggccatcag ctcgacgagg
    10981 gtgaagccac gctcgtcgct gcggagggaa tgcagtgaca tcaaagggct tatcggcgaa
    11041 cacacgccgc tactccaccc gcacgtgacc gcgggtggac gcaggtacga catgcgtcca
    11101 agaacgcaac gaggcgggcc gaagcccgcc tcgagagcga tgtgcgtgta ccgaacggcc
    11161 taccagtagt tgccgttgga gtcggccgcc gacttgcagg aaccctgacc gtgggtcgtg
    11221 cagtcgcgcg agaacgtgcc ggtcgtcgcc ttcgtgatcg tgaaggtgtt gcccgacttc
    11281 gaggtcgcgg tcacggcgta ggcgctcgcg ccattcggcg tgatcgtgac gctgttctgg
    11341 acaggcgtgc ccagcgagct cagcttcgcc gagtcacact gggtgtagtc ctcggtgtcc
    11401 gtgtagcagg actccatcgc ggagaccgtg ttacgcgcat cggacttcgc cgaggcgtcc
    11461 tgccccttct tctgctgacc caggaaggtc ggcagagcga tcgcggcaag gatgccgatg
    11521 atcaggatga cgacgaggag ctcgatcagc gtgaaaccgc tctcctcagt ctgaatacgc
    11581 tcgcggagcg tccgcagcat ggatcctcct tgggttagtc gcgccaggac gagcggatgg
    11641 cttccgttgc ctctggctct gcgggcggtt atcggtagaa gctcgcgcgc gctttaggtc
    11701 ggaacgctga ccacatggac gaacgattcg aaaaagcaac gaggcgggcc ggagcccgcc
    11761 tcgaacgtgt acggcgatta ggctcgccgg cttaccagta gttgccgttg gagtcggccg
    11821 ccgacttgca ggaaccctga ccgtgggtcg tgcagtcgcg cgagaacgtg ccggtcgtcg
    11881 ccttcgtgat cgtgaaggtg ttgcccgact tcgacgtcgc gctcacggcg taggcgctcg
    11941 cgccattcgg cgtgatcgtg acgctgttct gcaccggggt gcccagggcg ctcagcttcg
    12001 ccttgtcaca ctgggtgtag tcctcggtgt ccgtgtagca ggactccatc gcggagaccg
    12061 tgttacgtgc atcggacttg gccgacgcgt cctgcacctt cttctgctga cccaggaagg
    12121 tcggcagggc gatcgcggca aggatgccga tgatcaggat gacgacgagg agctcgatca
    12181 gcgtgaaacc ggactcctcg gactgaatgc gcatgcggag cgtccgcagc atgggtcctc
    12241 cttggattgg tcgcgccagg accatcggat gccacccagg agggcccggc tggcagctcg
    12301 gccgactccc cgaatcgggg gagccccgga gctttgcgcc cccgcctcgc gacgggtttg
    12361 cccttcaccg
//
LOCUS       DASVXS010000283        12691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_612042, whole genome shotgun sequence.
ACCESSION   DASVXS010000283 DASVXS010000000
VERSION     DASVXS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612042"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VF752_15965"
     CDS             complement(<1..863)
                     /locus_tag="VF752_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX8857090.1"
                     /translation="MQENGYRVLVKEKIADSGVDLLREHFDVDLGVDWDDAELESKIG
                     DYDAILIRSATKLTSDLIERGSRLKVIGRAGVGIDNVDLRAATKRGIIVANAPESNTI
                     AAAEHTLALMLALSRNVPQAHASLSTGHWERSKFGGMEVYEKTLGILGFGRIGTLVAE
                     RAKAFGMNVIAFDPFVSAERYRELGIEHAETSDDVYAVADIITLHLPKTPETTNWLDD
                     EAFSKMKDSVRIINVARGELVDHDALERALDSGKVAGAALDVFPQEPITEHPLFGMEG
                     VIVTPHLGASTA"
     gene            complement(871..2055)
                     /locus_tag="VF752_15970"
     CDS             complement(871..2055)
                     /locus_tag="VF752_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX8857091.1"
                     /translation="MADPYIKDYLMTAGPTPLPPLVSQVMAEPMLYHRAPAFVEVYAR
                     VLDRLRLPFETSSDVLCFTSSGSGGMESAAANLVRPGEPVLVASCGKFGQRWAELADS
                     FGGRTEHYEAEWGTKVDPAEVDRRLAETGAKLFFSTLSETSTGVVNDIAALTEVAHRH
                     GALIVVDAVSALGAIPVRQDEIGVDVVVAGSQKALMAPPGLGFASVNEAALERASENA
                     DQGRYYFDWAKTAKGQRKDPPDSPFTPAVGLFAALDIALGMIESEGLEQVFERHRLLG
                     RATREAVKALELDLFGPEDEGANVVTAIRLPEGIDGGKVPKIMRDQYRITIAGGQNQL
                     KGKIARIAHCGYFGAFDIITTVAALEMTLRDLGHDLELGAGVAAAQRVFLEAGVAAAT
                     PA"
     gene            complement(2099..3094)
                     /gene="ilvC"
                     /locus_tag="VF752_15975"
     CDS             complement(2099..3094)
                     /gene="ilvC"
                     /locus_tag="VF752_15975"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX8857092.1"
                     /translation="MIYDNDADLGKLQGKTVAILGYGSQGHAHALNLRDSGADVVVGL
                     REGSKSAEKARAEGLEVLSVADAASRGDVVMVLLPDEKQAEVWESDIKDGIAPGNLLL
                     FAHGFSIHFGTIEPGPEVDVGMVAPKGPGHLVRRQYVEGGGVPGLAAIHQDATGNARD
                     LVLAYAKGIGCTRAGVIETTFKDETETDLFGEQAVLCGGLTELVRAGYDTLTGAGYDP
                     RLAYFEVLHELKLIVDLMYEKGISGMRYSISNTAEYGDLTRGKRVIGEPSRQAMQEIL
                     GQIQSGEFAREWIAENRAGQENFQRMRSDEKNHEVETVGRELRSNMDWIDDSEDF"
     gene            3259..3654
                     /locus_tag="VF752_15980"
     CDS             3259..3654
                     /locus_tag="VF752_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857093.1"
                     /translation="MDPFFNHLAADEHNKDLLEAARRNGGVGASRRSVPRSVTVRYFE
                     ERDIEAIRRLAVLDGKIEPDGAVLVADVGGHIVAALPLLSGEAIADPFAPTADVVELL
                     KLRAAQLTPPRPRAPRGIFGRFPLVSRAA"
     gene            complement(3651..4118)
                     /locus_tag="VF752_15985"
     CDS             complement(3651..4118)
                     /locus_tag="VF752_15985"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX8857094.1"
                     /translation="MTERRLRIAIAVASLLGLGIAAYLTYVHYAHTTVLCVAGGGGCE
                     KVQNSDQSKLAGIPVAVLGLVGYAGIFGSALVRGEAARIGGALLALIGIGFSLYLTYA
                     EIFLIHAICQWCVASCVVMAILAALTCARLLLPDEGAARAPSGSRGELGARVG"
     gene            complement(4115..4897)
                     /locus_tag="VF752_15990"
     CDS             complement(4115..4897)
                     /locus_tag="VF752_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8857095.1"
                     /translation="MTQRAPTRKERRDQARAERVAREQEAARRAQFRRRLGILGGVVG
                     LAIVAVVVAIALSSSGGSHKSSTTGQTTSLFAGIPEKGITLGKPNAPVTLEEYGDLQC
                     PFCREYTLNALPTLVRDYVRTGKVKMVFHNLSFIGPDSVTAGRVAQAAGMQNKLWPFI
                     DRFYANQGQENTGYVTRDFLDKVASQVPGLDKQKLFSDADSAASQTGLAAANASANTR
                     GVKSTPRFFVSVNGKPAQELQPSSLTPGGFESQLNALLAKAT"
     gene            complement(4951..6312)
                     /gene="menE"
                     /locus_tag="VF752_15995"
     CDS             complement(4951..6312)
                     /gene="menE"
                     /locus_tag="VF752_15995"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEX8857096.1"
                     /translation="MSKPAEHIEFELPLRRSARTLPERVALICAGGEISYAELERRAQ
                     QAARRLAAHDVEAGDRVATTLPPGVDFSALLHAVAMLGASLVPLNTRLSEAERRWQLE
                     DSRARVLVDEPVGGEEADVPLRETFDPRAEHSLIYTSGTTSRPRAVSLTHGNQMASAI
                     ANAWNIGVDPDDRWLCVLPLFHVGGLAILVRSALYGTSALVRERFDVDEVRADLEEGR
                     ATVASFVSTMLRRLADAGLERAPRLRAALLGGGPVPRDLLEWAAARGLPVLQTYGMTE
                     TASQIATLRAEEALQLKGSAGRPLLGVEISTSPEGEILARGPMVAPGALGPDGWLHTG
                     DRGRLDEDGYLWVDGRLKDVIVTGGENVSCAEVEEALLSHPAVSDAGVAAEPDAEWGE
                     VVCAFVVASDGVTADELIAHCRARLAPYKAPRRVEFLDQLPRNPAGKLVRRDLPVRGK
                     TTT"
     gene            6329..7153
                     /gene="menB"
                     /locus_tag="VF752_16000"
     CDS             6329..7153
                     /gene="menB"
                     /locus_tag="VF752_16000"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX8857097.1"
                     /translation="MAVPDWKPAHEYEDIRYELADGIAKITINRPEVRNAFRPETVIE
                     LSDAFDRAREDTSVGAIILTGEGPDAFCSGGDQRVRGDTGYMTGGRGVGRFHVTDLHL
                     QMRRLPKPIVAMVAGYAIGGGHVLHVLCDLTIAAANARFGQTGPRVGSFDGGYGAALL
                     AEQVGVKKAKEIWFLCRQYDAQQALEMGLVNTVVPLDQLEEETVRWCREMLELSPFAL
                     RLLKASFNAAEDGIAGLQQLAHDANLLFYASDEAREGREAYKEKRKPDFGKFPKRP"
     gene            7150..8064
                     /locus_tag="VF752_16005"
     CDS             7150..8064
                     /locus_tag="VF752_16005"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX8857098.1"
                     /translation="MNSARTAAAAPAGALRLWLVAARPRTLPAAIAPVLVGTSLAIED
                     GTFRPLAFVCALIGSVFIQIGTNLSNDYSDARRGADTDDRLGPVRVTAGGLMPPRQVL
                     IGTYVAFGIAVGAGAYLIAIAGWQLLAVGLASILAGVLYTGGPRPYGYEGLGELFVFL
                     FFGVVAVVGSYYVQTEDLTWEAFALSVPVGLLASAILVVNNVRDIETDRRAGKHTLAV
                     KLGRRGARRLYAAMLLLAYAAPALIWLAGGLDAWLLLPWASLPLVPRLLRTVTTRTDG
                     PALNGALAGTGMLLALFSLLLSGGILLS"
     gene            8061..9080
                     /locus_tag="VF752_16010"
     CDS             8061..9080
                     /locus_tag="VF752_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX8857099.1"
                     /translation="MIRRLRLLSIPLKEPFPTASGVVSARELALLELEDADGAVGYGE
                     AAPLEHYDGVSIDEVVEALNGGNGEGPPQARAAEEMALLDLEARRAGRALGEPGADAI
                     PVNRTLPAGPPDRVAAHAVEAVRDGFSCFKVKTGLPDDRLRVGAVRAAIGPWPALRID
                     ANGSWTVDQAVEAIESLAEFDLQLVEQPCATLEELAEVRRRVAVPIAADESVRTPEDV
                     RTAAVLEACDVVNVKLASSGGFSAAREALREARRRGLASYLSSTLDGPWGIAAALQLA
                     SSEEVLLACGLATLDLFDSPLAGALPPPHDGLLAVPQGPGLGVEVDHETLASAVVDEL
                     PSRAS"
     gene            complement(9026..9523)
                     /locus_tag="VF752_16015"
     CDS             complement(9026..9523)
                     /locus_tag="VF752_16015"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8857100.1"
                     /translation="MEPGDIGEIVRLHGTLYAEEYGLDRRFEAEVAQNLGKAVRGGFP
                     REGEGVWLEEREGRLLGGIFLIRQSARIGRVRWFILTPEARGQGLGRRLLAELMQAAR
                     AYGYEHMWLETFSALEAAAHLYREAGFELRGSEESTRWGPEIVVQSYEARLGSSSTTA
                     EASVS"
     gene            complement(9532..10239)
                     /locus_tag="VF752_16020"
     CDS             complement(9532..10239)
                     /locus_tag="VF752_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8857101.1"
                     /translation="MLRPDLEIVFIPGFAQQGEAWQPVADRVGESYRSAYAKFATPDF
                     EGRLAELEGCESALVGYSLGGRLALHAALRGLAGPRALVLLGVSAGIDDPARRAERRV
                     ADEKLADWIEAHSIEEFTARWEANPVFASQPPELVAAQRPGRLAQAPRDLAITLRTAG
                     QGALDPVWNQLPELRCPVLAVAGELDEPYARAAERIATSVRNGRAALVPGAGHAAHLE
                     RPDEFAEILKSFLEEVE"
     gene            10246..11976
                     /gene="menD"
                     /locus_tag="VF752_16025"
     CDS             10246..11976
                     /gene="menD"
                     /locus_tag="VF752_16025"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX8857102.1"
                     /translation="MIPINRTYAPVHAFVDELARCGLRHAVTCPGSRNAPLALTLAGD
                     ERIEAISVLDERSAGFLALGIAKTERRPVVVTCTSGTAAANLLPAIVEARESHVPLIV
                     LTADRPPELRDVGAGQAIDQIKLFGSFPKWFTEVGTHPPTRATGIHYRALACRAWFTS
                     EDGRPGPVHLNFPLREPLAPVPEHLDPEPWQGRPDGRPWTVVTEVPSDPSPTVIELLE
                     RDVLESETGVIVCGPTLEQIPVAAARLAAATGWPILAEPASGVRCGPHDRSHVIAHYD
                     ALLRSERFVDAHRTDLVIRVGDTPTSKPLRAWLAGTRQIVIDRQAGWHEPTRRAERLV
                     TAAADSTLEMLAAALETEPPTRASWLASWRAADAVVTPALAAAPDPFEPKAFSALADA
                     LSDGAIVWLGSSMPVRDVETFFPQTETALRFVANRGANGIDGTVSSAVGAALAGPDPC
                     VLLTGELALLHDLGGALAARRHGANVRIVCINNGGGGIFDFLPVATHADPGHYREHIV
                     TPAGVDLATVASLAGLAHVRAETPDQVRRAISGPALVEVRTDREDNVRMHREVYARVD
                     AALSDALAQA"
     gene            11991..12380
                     /locus_tag="VF752_16030"
     CDS             11991..12380
                     /locus_tag="VF752_16030"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX8857103.1"
                     /translation="MYDRDCGFCRWSLGRVLALDRRRALEPVALDEPEADRLLEGMPN
                     DDKIASWHLVAPDGRHWSGGDAFAPLAEALGHPGARRALEPLRPLLRVGYRLVAGNRN
                     VWGKLISDDAKRRADARIEAARAPIPS"
     gene            complement(12373..>12691)
                     /locus_tag="VF752_16035"
     CDS             complement(12373..>12691)
                     /locus_tag="VF752_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX8857104.1"
                     /translation="LEHPIAVLELAGRLHPTPAVGGEPCERAQPLITALEGLDRGWYA
                     GALGWTDLAEDGELCVAIRCALLRGEIARLYAGNGITRDSDPAAELAETEHKLGALLP
                     LLS"
BASE COUNT         1789 a         4584 c         4392 g         1926 t
ORIGIN      
        1 gcggtggacg cgccgaggtg cggcgtgacg atcacgccct ccatcccgaa cagcggatgc
       61 tcggtgatcg gctcctgcgg gaacacgtcg agcgccgcgc cggccacctt gcccgagtca
      121 agcgcgcgct cgagcgcgtc gtggtcgacc agctcgccgc gcgcgacgtt gatgatgcga
      181 acgctgtcct tcatcttcga gaacgcctcg tcgtcgagcc agtttgtggt ctcgggcgtc
      241 ttcggaaggt gcagcgtgat gatgtcggca accgcgtaca cgtcgtcgga agtctccgca
      301 tgctcgatgc cgagctcgcg gtagcgctca gccgaaacga acgggtcgaa cgcgatcacg
      361 ttcatgccga acgccttcgc gcgctcggcc acgagcgtgc cgatgcggcc gaagccgaga
      421 atcccgagcg tcttctcgta gacctccatg ccgccgaact tcgagcgctc ccagtggccc
      481 gtggacagcg acgcgtgcgc ctgcggcacg ttgcggctga gcgcgagcat cagcgcgagc
      541 gtgtgctcgg cggcggcgat cgtgttcgac tccggcgcgt tcgcgacgat gatgccgcgc
      601 ttcgtggccg cccgcaggtc gacgttgtcg atccccacgc ccgcgcgccc gatcaccttc
      661 aggcgcgagc cgcgctcgat caggtccgag gtgagcttcg tggcggaacg gatgaggatc
      721 gcgtcgtagt cgccgatctt gctctcgagc tcggcgtcgt cccagtccac gcccaggtcc
      781 acgtcgaagt gctcgcgcag gaggtccacg ccggaatcgg cgatcttctc cttgacgagg
      841 acgcggtacc cgttctcttg cacggctgcc ctaggccggt gtcgcggcgg ccactcccgc
      901 ctcgaggaag acccgctgcg cggcggccac gccggcgccg agctcgaggt cgtgccctag
      961 gtcgcgcagc gtcatctcga gcgccgcgac cgtggtgatg atgtcgaagg cgccgaagta
     1021 gccgcagtgc gcgatgcgag cgatcttgcc cttgagctgg ttctgcccgc ccgcgatcgt
     1081 gatgcggtac tggtcgcgca tgatcttcgg gaccttcccg ccgtcgatgc cctcgggcag
     1141 cctgatcgcg gtgaccacgt tggcgccctc gtcctcaggg ccgaagaggt cgagctcgag
     1201 cgccttgacg gcctcgcgcg tggcgcggcc gagcagtcgg tggcgctcga agacctgctc
     1261 gaggccctcc gactcgatca tcccgagcgc gatgtcgagc gcggcgaaca ggccgacggc
     1321 gggcgtgaac gggctgtcgg gcgggtcctt gcgctgaccc ttggcggtct tcgcccaatc
     1381 gaagtagtag cgaccctggt cggcgttctc cgaagcgcgc tcgagcgccg cctcgttcac
     1441 gctcgcgaag ccgagccccg gtggcgccat cagcgccttc tgcgagcccg cgaccacgac
     1501 gtcgactccg atttcgtcct gccgcaccgg gatcgcgccc agagccgaca cggcgtcgac
     1561 cacgatcagc gccccgtgcc ggtgcgcgac ctcggtgagc gcggcgatgt cgttcacgac
     1621 gccggtcgag gtctcagaga gcgtgctgaa aaagagcttc gcgccggtct cggcgagccg
     1681 gcggtcgacc tcggcgggat cgaccttcgt gccccactcc gcctcgtagt gctcggtcct
     1741 ccctccgaac gaatcggcca gctcggccca gcgctgaccg aacttgccgc acgaggcgac
     1801 gagcaccggc tcgcccggcc ggaccaggtt cgccgcggcc gactccatgc ctcccgagcc
     1861 ggacgaggtg aagcagagca cgtcgctgct cgtctcgaac ggcagccgca ggcgatcgag
     1921 cacccgcgcg tacacctcca caaacgcggg cgcgcggtgg tagagcatgg gctccgccat
     1981 cacctgcgac acgagcggcg gcaggggcgt gggccccgcc gtcatcaggt agtccttgat
     2041 gtaggggtcg gccatcgaac gggccacgat agcggacgga ccggcgggcg ctgacccgct
     2101 agaagtcctc tgaatcgtcg atccagtcca tgttggagcg cagctcgcgc ccgaccgtct
     2161 cgacctcgtg gttcttctcg tcggagcgca tgcgctggaa gttctcctgg ccggcgcggt
     2221 tctccgcgat ccactcgcgc gcgaactcgc ccgactggat ctgacccagg atctcctgca
     2281 tggcctggcg gctcggctcg ccgatcacgc gctttccgcg cgtcaggtcg ccgtactccg
     2341 ccgtgttcga gatcgagtag cgcatgccgc tgatgccctt ctcgtacatc aggtccacga
     2401 tcagcttcag ctcgtggagc acctcgaagt acgcgagtcg cgggtcgtag ccggcgcccg
     2461 tgagcgtgtc gtagcccgca cgcaccagtt cggtgagccc gccgcacagc acggcctgct
     2521 cgccgaagag gtcggtctcc gtttcgtcct tgaacgtggt ctcgatcact cccgcgcgcg
     2581 tgcaaccgat gccctttgcg tacgcgagca cgaggtcgcg cgcgttgccc gtggcgtcct
     2641 ggtggatcgc cgccaggcct ggcacgcctc cgccctcgac gtactggcgc cgcaccaggt
     2701 ggcccgggcc cttcggcgcg accatgccga cgtcgacctc ggggccaggc tcgatcgtcc
     2761 cgaagtggat cgagaagccg tgggcaaaca gaagcaggtt gcccggggcg atcccatcct
     2821 tgatgtcgga ctcccacacc tcggcctgct tctcgtccgg caggagcacc atgaccacgt
     2881 cgccgcgact ggcggcgtcg gccactgaaa gcacctcgag gccctcggct cgggccttct
     2941 ccgccgactt cgagccttcg cgcaggccga ccaccacgtc ggccccggag tcgcgcagat
     3001 tgagggcgtg cgcgtggccc tgcgacccat aaccgaggat cgcgacggtc tttccctgga
     3061 gcttgccgag atcggcgtcg ttgtcgtaga tcacggaggc gactctattg ggttgtcaca
     3121 ctcggccccg cttcggcgtt ctagagggtg agtcacgagg cgccagcttt gaccggtgaa
     3181 cgtttgacag tggttacgta accggtataa tccccatacg agttataccc gagggcgcgg
     3241 cggaaccagg aggcagcaat ggatccgttc ttcaaccatc tggcggctga cgaacacaac
     3301 aaggacctcc tcgaggcagc acgccggaac ggcggcgtcg gcgcttcccg ccggagcgtc
     3361 cctcgtagcg tgacggtgcg ctacttcgag gagcgcgaca tcgaggcgat ccgccggctg
     3421 gccgtgctcg acggcaagat cgagccggac ggggcggtcc tcgtggccga cgtgggaggc
     3481 cacatcgtcg ccgcgctccc cctgctgagc ggcgaggcga tcgccgaccc gttcgccccg
     3541 accgccgacg tcgtggagct gctcaagctc cgcgccgcgc agctgactcc cccgaggccc
     3601 cgtgcccctc gcggcatctt cgggcggttc cctctggtca gccgcgctgc ctagccgacg
     3661 cgggcgccga gctccccgcg actaccgctc ggcgcccgcg cggcaccctc gtcggggagc
     3721 agcaggcgcg cgcacgtaag cgccgcgagg atcgccatca caacgcagct cgcgacgcac
     3781 cactggcaga tcgcgtggat cagaaagatt tcggcgtacg tgaggtagag gctgaagccg
     3841 atgccgatga gtgcgagcag cgcgccgccg atgcgcgcgg cctctccgcg cacaagcgct
     3901 gagccgaaga tgcccgcata gcccacgagc ccgagcaccg caaccgggat tccggcgagc
     3961 ttcgactggt cggagttctg gaccttctcg cagccgccac cgcccgcaac gcagagcacg
     4021 gtggtgtgcg cgtaatgcac gtacgtgagg taggcggcga tcccgagacc gagcagcgaa
     4081 gccaccgcga tggcgattcg cagccgccgc tcggtcacgt cgccttggcg agaagcgcgt
     4141 tgagctgcga ttcgaagccg ccgggcgtga gggagctcgg ctgcagctcc tgtgccggct
     4201 tgccgttcac cgagacgaag aacctgggcg tggatttgac gccgcgcgtg ttagcgctcg
     4261 cgttcgccgc ggcgagaccc gtctgtgagg ccgcggagtc ggcgtcgctg aagagcttct
     4321 gcttgtcgag gcccggcact tgcgacgcga ccttgtcgag gaagtcacga gtcacgtagc
     4381 cggtgttctc ttggccctgg ttcgcgtaga agcggtcgat gaacggccaa agcttgttct
     4441 gcatgcctgc tgcctgcgcg acccggccgg ccgtgacgga gtccgggccg atgaacgaca
     4501 ggttgtggaa gaccatcttc accttgccgg tgcgcacgta gtcgcggacg agcgtcggca
     4561 gcgcattgag cgtgtactcg cggcagaacg ggcactgcag gtcgccgtac tcctcgaggg
     4621 ttacgggagc gttcggcttt ccgagcgtta tgcccttctc ggggatgccg gcgaagagcg
     4681 atgtggtctg tccggtggtc gagctcttgt gggagccgcc gctcgagctg agcgcgatcg
     4741 cgaccaccac ggccacgatc gccaggccaa cgactccgcc caggatcccg aggcggcgcc
     4801 tgaactgggc gcggcgggcg gcctcctgct cacgagctac ccgttctgcc cgtgcctggt
     4861 cgcggcgttc cttgcgtgtc ggcgcgcgct gcgtcaagaa tcacaagggt agctagggcg
     4921 gcctaaagag agcttgagaa ttcgcgcggc tcacgtcgtg gttttgccgc ggaccggcag
     4981 atccctgcgc accagcttcc cggccgggtt ccgcggtagt tggtcgagga attcgacccg
     5041 acgcggcgcc ttgtacgggg cgagccgcgc gcggcagtgc gcgatcagtt cgtcggccgt
     5101 cacgccgtcg ctcgcgacca cgaacgcaca caccacctcc ccccactccg cgtccggctc
     5161 ggccgccacg ccggcgtcgc tcacggcggg gtgcgagagg agcgcctcct ccacctccgc
     5221 gcacgagacg ttttcgccgc ccgtcacgat cacgtccttc aggcgcccgt caacccagag
     5281 gtagccatcc tcgtcgagcc gcccgcggtc gcccgtgtgg agccagccgt ccggtccgag
     5341 ggcgccgggc gcgaccatcg ggccgcgcgc gaggatctct ccctccggcg acgtcgagat
     5401 ctccacaccg agcagcggcc gacctgcgga tcccttcagc tggagtgctt cctcggcgcg
     5461 cagcgtcgcg atctgcgagg cggtctccgt catcccgtag gtctggagca cgggaaggcc
     5521 gcgcgccgcc gcccactcga gcaggtcgcg cggcacgggc ccacctccga gcagcgccgc
     5581 gcgcagccgc ggggcgcgct cgaggcccgc gtcggccagc cggcgcagca ttgtggagac
     5641 gaaagaggcc acggtcgccc gcccctcctc caggtcggct cgcacctcgt ccacgtcgaa
     5701 gcgctcgcgc accagcgccg acgtgccgta cagcgccgag cgcaccagga tcgcgaggcc
     5761 gcccacgtgg aagagcggca gcacgcagag ccagcggtca tcggggtcga cgccgatgtt
     5821 ccaggcgttc gcgatcgcgc tcgccatctg gttgccgtgc gtgagcgaca cggcgcgtgg
     5881 ccggctcgtg gtgccggacg tatagatgag cgagtgctcc gcgcgcgggt cgaacgtctc
     5941 gcgcagcggc acgtcggcct cctcgccgcc aaccggctcg tccacgagta cacgcgcgcg
     6001 cgagtcctcg agctgccagc ggcgctccgc ctcgctcagg cgcgtgttca gcggcacgag
     6061 cgaagcaccg agcatcgcca ccgcgtggag cagcgcggag aagtcgacgc ccggcggaag
     6121 cgtcgtcgcc acgcggtccc cggcctccac gtcgtgtgcg gccaggcggc gcgccgcctg
     6181 ctgcgcccgg cgctcaagct cggcgtacga gatctcgccg ccggcgcaga tcagcgcgac
     6241 gcgctcgggg agcgtgcgcg ccgaccgcct gaggggcagc tcgaactcaa tatgctcggc
     6301 cggcttggac acagcagaaa gggacagctt ggcagtaccg gactggaagc ccgcccacga
     6361 gtacgaggac atcaggtacg agctcgcgga cggcatcgcg aagatcacca tcaaccgccc
     6421 cgaggttcga aacgccttcc gcccggagac cgtgatcgag ctctcggacg cgttcgaccg
     6481 agcccgggag gacacgtcgg tcggcgcgat catcctcacc ggcgagggtc ccgacgcctt
     6541 ctgctccggc ggcgaccagc gcgtgcgcgg cgacaccggc tacatgaccg gcgggcgagg
     6601 cgtgggccgc ttccacgtga ccgatctgca cctgcagatg cgccgcctgc ccaagccgat
     6661 cgtggcgatg gtggccggct atgcgatagg cggcgggcac gtgctgcacg tcctgtgcga
     6721 cctcacgatc gccgccgcca acgcccgctt cggccagacc ggaccgcgcg tggggagctt
     6781 cgacggcggc tacggcgcag cgctcctcgc ggagcaggtg ggcgtgaaga aggcgaagga
     6841 gatctggttc ctctgccgcc agtacgacgc gcagcaggcg ctcgagatgg ggctcgtaaa
     6901 caccgtcgtg ccgctcgacc agctcgagga ggagacggtg cgctggtgcc gcgagatgct
     6961 ggagctctcc cccttcgccc tgcggctgct gaaggcgagc ttcaacgccg ccgaggacgg
     7021 catcgccggg ctccagcagc tcgcgcacga cgcgaacctt ctcttctacg cgagcgacga
     7081 ggcccgtgaa ggccgcgagg cctacaagga gaagcgcaag cccgacttcg gcaagttccc
     7141 gaagcggccg tgaactcggc gcgcacagcg gcagccgcac cggccggcgc gctgcgcctc
     7201 tggctcgtgg cggcgcggcc gcgcacgctg cccgccgcga tcgcgcccgt gctcgtgggc
     7261 acctcgctcg cgatcgagga cgggacgttc cggccgctcg cattcgtatg cgcgctgatc
     7321 ggctcggtct tcatccagat cgggacgaac ctctcgaacg actattccga cgcccgccgc
     7381 ggcgccgata ccgacgaccg gctagggccg gtgcgcgtga cggccggagg cctgatgccg
     7441 cctcggcagg tcctgatcgg cacgtacgtt gcgttcggca tcgcagtcgg cgcgggcgcg
     7501 tacctcattg cgatcgcggg ctggcagctg ctcgcggtcg ggctcgcctc gatcctcgcc
     7561 ggcgtcctct acacgggcgg tccgcgccct tacggctacg agggcctcgg cgagctgttc
     7621 gtgttcctct tcttcggcgt ggtcgccgtc gtgggctcct actacgtgca gaccgaggac
     7681 ctcacgtggg aggcgttcgc cctctcggtt cccgtggggc tgctcgcgtc ggcgatcctc
     7741 gtggtgaaca acgtgcgcga catcgagacc gatcgacgtg ccggtaagca cacgctcgcg
     7801 gtgaagctcg ggcgccgggg agcgcgcagg ctctatgccg cgatgttgct gctcgcctac
     7861 gcggcgcccg cgctcatctg gctcgccggc gggctcgatg cctggctgct tctgccctgg
     7921 gcctcgctgc cgctcgtgcc ccggctgctg cgcacggtca ccacacgcac cgacgggcct
     7981 gcgctcaacg gcgcgctcgc cgggaccggc atgctgctcg cgctcttttc ccttctgctc
     8041 tcaggcggca ttctgttgtc gtgatccgca ggctgcggct gctctcgatc ccgctgaagg
     8101 agccgttccc cacggcgagc ggagtggttt ccgcgcgcga gctggcgctg ctcgagctgg
     8161 aggacgcaga cggtgcagtc ggctacggcg aggcggcgcc gctcgagcac tacgacggtg
     8221 tctcgatcga cgaggtggtc gaggcactca acggcggcaa cggcgagggc ccgccgcagg
     8281 cgcgggccgc ggaggagatg gcactgctcg acctggaggc gcggcgcgcg gggcgtgcgc
     8341 tcggggaacc tggcgccgat gccatcccgg tgaaccgcac gcttcccgct ggtccgcccg
     8401 acagggtcgc cgcgcacgcc gtcgaggccg tccgcgacgg cttttcctgc ttcaaggtca
     8461 agacggggtt gcccgacgac cggctgcgcg tgggtgccgt ccgtgccgcg atcggcccgt
     8521 ggcccgcgct gcggatcgac gcgaacgggt cgtggaccgt ggaccaggcc gtggaggcga
     8581 tcgagtcgct cgccgaattc gacctgcagc tcgtggagca gccgtgcgcg accctcgagg
     8641 agcttgccga ggtgcgccgc cgggtggcgg tgccgatcgc cgccgacgag tccgtgcgca
     8701 cgccggagga cgtgcggacc gcggccgtgc tcgaggcctg cgacgtggtg aacgtgaaac
     8761 tggcgtcgag cggaggcttc agcgcggcgc gcgaggcgct gcgcgaggcg cggcgccgcg
     8821 ggctcgcgtc gtacctctcg agcacgctcg acggaccgtg gggaatcgcc gcggcgctgc
     8881 agctggcgtc gtcggaggaa gtgctgctcg cgtgcggtct cgcgacgctc gacctgttcg
     8941 actcgccgct cgcgggcgcg ctgccaccgc cgcacgacgg gctgcttgcc gtgccccagg
     9001 gcccggggct cggcgtggaa gtggatcacg agacactcgc ctcggccgtc gtcgacgagc
     9061 tcccgagccg cgcctcgtag gactgcacca cgatctccgg tccccagcgg gtcgactcct
     9121 ccgacccgcg tagctcgaag cctgcttccc gatacagatg ggccgccgcc tcgagcgcgc
     9181 tgaaggtctc gagccacatg tgctcgtagc cgtaggcgcg cgccgcctgc atcagctcgg
     9241 cgagcagccg gcgtccgagg ccttgcccgc gcgcctcggg agtgaggatg aaccagcgca
     9301 cgcgtccgat gcgcgcgctc tggcggatga ggaagatccc gccgagcagg cgcccctcgc
     9361 gctcctcgag ccagactccc tcgccctcgc gcgggaaccc gccgcgcacc gctttgccga
     9421 ggttctgcgc cacctcggcc tcgaagcgcc ggtcgaggcc gtactcctcg gcgtagagcg
     9481 tgccgtggag ccgcacgatc tcaccgatgt cgccgggctc caaggaagtc ctcactccac
     9541 ctcctcgagg aagctcttca ggatctcggc gaactcgtcc ggccgctcca ggtgggcggc
     9601 gtgcccagcg cccggcacga gggcggcacg cccgttccgc acgctggtgg cgatgcgctc
     9661 ggctgcgcgt gcgtagggct cgtcgagctc accggcgacg gcgagcacag ggcagcgcag
     9721 ctccgggagc tggttccaca cggggtcgag cgcgccctgg cccgcggtgc ggagggtgat
     9781 cgccaggtcc ctcggcgcct gcgcgaggcg cccggggcgc tgggctgcga ccagctcggg
     9841 cggctggctc gcgaagactg ggttcgcctc ccagcgcgcg gtgaactcct cgatcgagtg
     9901 ggcctcgatc cagtccgcga gcttttcgtc ggccacgcgc cgctccgctc ggcgcgccgg
     9961 gtcgtcgatg cccgcgctca cgccgagcag cacgagcgca cgaggacctg caaggccgcg
    10021 cagcgccgcg tgcagcgcca ggcggccgcc gagcgagtag ccaaccagtg ccgactcgca
    10081 cccctccagc tcggccagcc ggccctcgaa gtcaggtgtg gcgaactttg cgtaagcgct
    10141 ccgatagctt tcgcccactc tgtctgcgac tggctgccac gcttcgccct gctgcgcgaa
    10201 cccagggatg aacacgatct cgaggtcggg ccggagcacg cgcacatgat cccgatcaac
    10261 cgcacctacg cgccggtcca cgccttcgtc gacgagctcg ctcgctgtgg cctgcgccac
    10321 gccgtcacct gcccgggttc gcgcaacgcg ccgctcgcgc tcaccctcgc cggcgacgaa
    10381 cgcatagagg cgatctcggt gctggacgag cgctccgccg gattcctcgc tctcgggatc
    10441 gcgaagaccg agcgccggcc ggtggtggtc acgtgcacct ccggcacggc ggcggcgaac
    10501 ctgcttccgg cgatcgtcga ggcgcgcgag tcgcatgtcc cactgattgt gctcaccgcg
    10561 gaccggccgc cggagctccg cgacgtgggc gccggccagg caatcgacca gatcaagctc
    10621 ttcggttcgt tcccgaagtg gttcaccgag gtcgggaccc accctcccac tcgcgcgacc
    10681 ggcatccact accgcgcgct ggcctgcagg gcctggttca cgtcagagga cggaaggcct
    10741 gggcccgtcc acctgaactt cccgctgcgc gagccgctcg cgccggtgcc cgagcatctc
    10801 gaccccgagc cgtggcaggg tcgcccggac ggccgcccgt ggacggtcgt gacggaggtg
    10861 ccgagcgatc cctcgcccac cgtgatcgag ctgctcgagc gcgacgtgct cgagtccgag
    10921 accggcgtga tcgtgtgcgg cccaacgctc gagcagatcc cggtggcggc cgcacgcctt
    10981 gcggccgcga caggctggcc gatcctcgcc gagccggctt cgggcgtgcg ttgcggccca
    11041 cacgaccgct cgcacgtgat cgcccactac gacgccctgc tccggtccga gcgcttcgtg
    11101 gacgcgcatc gcaccgacct cgtcatccgc gtgggcgaca cgccgacctc caagccgctg
    11161 cgcgcatggc tcgcgggcac gcggcagatc gtgatcgacc gccaggcggg atggcacgag
    11221 ccgacgcgcc gggcggagcg cctcgtcacc gcggcagcgg actccacgct cgagatgctg
    11281 gctgccgcgc tcgagactga gccgcccaca cgtgcgtcct ggctcgcgtc gtggcgcgcc
    11341 gcggacgcgg ttgtgacgcc ggcgctcgcg gccgcgcccg atccgttcga gccgaaggcc
    11401 ttttccgcgc tcgccgacgc cctgtcggac ggcgccatcg tgtggctcgg ctcctcgatg
    11461 ccggttcgcg acgtggagac cttcttccca cagaccgaga ccgcgctgcg ctttgtcgcc
    11521 aaccgcgggg ccaacggcat cgacggcacg gtttcctccg cggtcggcgc ggcgctcgcc
    11581 gggccggatc cgtgcgtgct gctcaccggc gagctggccc tcctacatga cctcggcggc
    11641 gcgctggccg cccgccgcca cggcgcgaat gttcggatcg tctgcataaa caacgggggt
    11701 ggcgggatct tcgacttcct gccggtcgcc acgcacgccg atcccggcca ctatcgcgaa
    11761 cacatcgtca cgcccgcggg tgtggacctc gccaccgtcg cttcgctcgc gggcctcgcg
    11821 cacgtgcggg cggagacgcc agaccaggtg cggcgcgcga tctcgggccc ggcgctcgtc
    11881 gaggtgcgca ccgaccgcga agacaatgtg cgcatgcatc gagaggtgta tgcacgggtg
    11941 gacgcggcgc tatcggacgc actggcccag gcatagagcg ctggaggatc ctgtacgacc
    12001 gcgactgcgg cttctgccgc tggtcactcg ggcgggtgct cgcgctcgac aggcgccgcg
    12061 cgctcgagcc cgtcgcgctc gacgaacccg aggccgaccg cctgctcgaa ggcatgccga
    12121 acgacgacaa gatagcttca tggcacctcg ttgcgccgga cggccgccac tggtccggcg
    12181 gtgacgcctt cgcgccgctc gccgaggcgc tcggccaccc gggtgcgcgg cgcgcgctcg
    12241 agccgctgcg tcccctgctc cgagtcggct accggctcgt ggccggcaac cgcaacgtct
    12301 ggggcaagct cataagcgac gacgcgaagc gacgtgccga cgcgcgcatc gaggccgcac
    12361 gcgcgcctat ccctagctga gaagcggaag cagcgcgccg agcttgtgct cggtctccgc
    12421 cagttcagcg gcggggtccg agtcgcgcgt tatcccattg ccggcgtaca gccgggcgat
    12481 ctcgccacgc agtagcgcgc accggatcgc cacgcacagc tcgccgtcct cggcgaggtc
    12541 cgtccagccg agcgcgccgg cgtaccagcc gcggtcgagg ccctcgagcg cggtgatcag
    12601 cggctgggcc ctttcgcagg gctcgccgcc gacggccggg gtcgggtgga gtcgcccggc
    12661 cagctccagg accgcgatcg gatgctccag c
//
LOCUS       DASVXS010000284         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1261765, whole genome shotgun sequence.
ACCESSION   DASVXS010000284 DASVXS010000000
VERSION     DASVXS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261765"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1550)
                     /locus_tag="VF752_16040"
     CDS             complement(138..1550)
                     /locus_tag="VF752_16040"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8857105.1"
                     /translation="MASSGGNGQAKRAALYGGLPDPGPPGFAARMLDFADELRREGMH
                     VGTSELLDAFAALREVPWTEREDFKEALAATLAKSQEDRRVFEILFDRFFFRAVERKA
                     VEKGLKEERFEGAERLDMDELRQRIRDAIREGSDGEMRDMARLAIAAFGRQGEGSGVI
                     GVDVQRIRRTLGLKGERDAAALPDPDSVPQERLKEFERHLRRELERQMIERTQSLPPS
                     KPLQEFDRALPSAPMQDLAAVHRVVAQLKRRLATRGHEQRGRKQAQLVDVRRTMRASL
                     ETGGVPLRLKYRPKRPRRPELYVLCDVSTSVTSASVFFLSVLHALHDSFRKLRSFVFV
                     ERISEVTDVFERERSFEAVSRAIAADAGVADVSGYTDYGRVWLEFLDNVVDDLDPRST
                     VIVLGDARTNGREPHARAFARVSEHAGRMFWMNPEPKLYWNYGDSVMAAYEKYCDGVF
                     ECWTVKQLEVFVNALSLDRR"
     gene            complement(1519..2418)
                     /locus_tag="VF752_16045"
     CDS             complement(1519..2418)
                     /locus_tag="VF752_16045"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8857106.1"
                     /translation="MTSEPVSIDETRQALADVGYLADEAASLVSFLAAKLGKPVLVEG
                     PAGVGKTELAKALSRATGRKLIRLQCYEGLDEAKALYEWNYRKQLLRIQVEASETGWE
                     QVQDDIFSEDFLLTRPLLEAIAEREPVVLLIDEIDKTDQEFEAMLLEVLSDFQISIPE
                     LGVVEAKSQPIVLLTSNNTRELTEALKRRCLYLWLDYPSVERELEIVRLHAPDLSEDL
                     ARKLVEIMHMVRNLDLKKPPSIAESIDWARALLLLGAEDIDRSMLERTMNIIVKHRAD
                     LDVVAERVGVKLTDGLIGRQRAS"
     gene            2444..2629
                     /locus_tag="VF752_16050"
     CDS             2444..2629
                     /locus_tag="VF752_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857107.1"
                     /translation="MWHLLPLGASEVQTSRYILLLVIGLLVAVAGHISRTKTLIALGL
                     LLIVAGTFLFQFEVRAQ"
     gene            complement(2791..4131)
                     /locus_tag="VF752_16055"
     CDS             complement(2791..4131)
                     /locus_tag="VF752_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8857108.1"
                     /translation="MTGTLPDGPREPAVLQTARYLTRPFGSLDSAYERYGPVFTINLW
                     PVGRMVLLADPDAVKAVWSKDRVNTLPKGRDVLLRPLLGPRSLLLQEGAEHLRRRKLM
                     LPPLHGERMRGYVETMREATERDMARWPVGRPFPLIERMQAITLDVILRAVFGTTGGP
                     REDELRTVLRDILNWSVGFRANVMLALAQTPELLMRTPLGRLVARADELLHAEIEERR
                     RADDLEEREDILSLLALARDEDGSPLSDDELRDQLMTLLAAGHETTATGLAWAFDALF
                     RHPDVMDRLRDEITSDDDSYLQAVVQETLRVRPVVPEVGRVATEETRTNGHVLPPGTP
                     TLASVQLIHRRADLYPEPLAFRPERFLDDKPATYSWIPFGGGTRRCIGGAFAELEMRV
                     VIRTILERADLLPGSEAAEPPARRPVTMAPANGTPAVLASAVRRRSSAVSAVAA"
     gene            4224..>4953
                     /locus_tag="VF752_16060"
     CDS             4224..>4953
                     /locus_tag="VF752_16060"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX8857109.1"
                     /translation="MAEVQSGLEGVVAFATQIAEPDKEGSALRYRGVDIEDLVGRVRF
                     EHVWGLLVDGGYLPGLPPAEPHPLAIRSGDARVDIQSALAMLAPEWGLGQLLDISDEQ
                     ARRDLARASVMALSFVAQSARGPGQPPIPQSEVDKAATIPERFLIRWRGEADPDHARA
                     IDAYWISAAEHGMNASTFTARTVASTGADAAASLSAAVGALSGPLHGGAPSRVLEMIG
                     AVEESGDPESYVKQVLDSGGRLMGF"
BASE COUNT          756 a         1757 c         1670 g          770 t
ORIGIN      
        1 agacggaccg acgagcgcga cgatctcgcc ttcgccgacc gtcagcgaca gatcgtcaag
       61 cgcgaccagg tcgccgaagc gacgcgtgac accgcgcacc tcggccgtcg cttgcgttgg
      121 ctccgttgtc acgccctcta acgacggtca agcgacagcg cattcacgaa tacctcgagc
      181 tgcttgaccg tccagcactc gaagacgccg tcgcagtact tctcgtatgc ggccataacc
      241 gaatcgccgt agttccagta cagcttcggc tccgggttca tccagaacat ccggccggcg
      301 tgctccgaga ctcgcgcaaa cgcccgggcg tggggctcgc ggccgttggt gcgggcgtcg
      361 ccgagcacga tcacggtcga gcgcgggtcg aggtcgtcga ccacgttgtc gaggaactcg
      421 agccacacgc gcccgtagtc ggtgtagccg gacacgtcgg ccacgccggc gtcggccgcg
      481 atcgcgcgcg agacggcctc gaacgagcgc tcgcgctcga acacgtcggt cacctcgctg
      541 atccgctcga cgaacacgaa cgagcgcagc ttccggaagc tgtcatgaag cgcgtgcagc
      601 accgagagga agaagacgct ggcgctcgtg acgctcgtcg agacgtcgca gagcacgtat
      661 agctcggggc ggcgcgggcg cttcgggcgg tacttcagcc gcaggggcac gccgccggtc
      721 tcgagtgacg cgcgcatcgt tcggcgcacg tccacgagct gcgcctgctt gcggccgcgc
      781 tgctcgtggc cgcgcgtggc gagccggcgc ttcagctgcg ccaccacgcg gtgcaccgcg
      841 gcgaggtcct gcatcggcgc gctcggcagt gcgcggtcga actcctgcag cggcttggag
      901 ggcggcagcg actgcgtgcg ctcgatcatc tggcgctcga gctcgcgccg caggtggcgc
      961 tcgaattcct tcaggcgctc ctggggcacg gagtcggggt cgggcagcgc cgctgcgtcg
     1021 cgctcgccct tcaggccgag cgtgcggcgg atgcgttgca cgtcaacgcc gatcacgccc
     1081 gagccctcgc cctggcgccc gaaggcagcg atcgcgaggc gcgccatatc ccgcatctcg
     1141 ccgtcagagc cctcgcggat cgcgtcgcgg atgcgctggc gcagctcgtc catgtcgagg
     1201 cgctcggcgc cctcgaagcg ctcctccttc aggcccttct cgaccgcctt gcgctcgacc
     1261 gcgcggaaga agaagcgatc gaacaggatc tcgaacacgc gccggtcctc ctgcgacttg
     1321 gccagtgtgg cggcgagcgc ctccttgaaa tcctcgcgct cggtccacgg cacctcgcgc
     1381 agcgcggcga acgcatcgag caactcgctc gtgcccacgt gcatgccctc gcgccgaagc
     1441 tcgtcggcga aatcgagcat gcgcgcggcg aagccgggcg gcccggggtc gggcagccct
     1501 ccgtacagcg cggcgcgctt agcttgcccg ttgccgcccg atgaggccat cggtcagctt
     1561 cacgcccacg cgctcggcaa ccacgtcgag gtcggcccgg tgcttcacga tgatgttcat
     1621 cgtgcgctcg agcatcgagc ggtcgatgtc ctcggcgccg agcagaagca gcgcgcgcgc
     1681 ccagtcgatc gactccgcga tcgacggcgg cttcttgagg tcgaggttgc ggaccatgtg
     1741 catgatctcg acgagcttgc gcgcgagatc ctccgacaga tcgggcgcgt gcagccgcac
     1801 gatctcgagc tcacgctcga cgctggggta gtcgagccac aggtagaggc agcgccgctt
     1861 gagcgcctcg gtcagctcgc gcgtgttgtt cgaggtgagc aacacgatcg gctggctctt
     1921 cgcctccact acgccaagct cagggatcga gatctggaag tccgacagaa cctcgagcag
     1981 catcgcctcg aactcctgat cggtcttgtc gatctcgtcg atcagcagca cgaccggctc
     2041 acgctcggcg atcgcctcga gcaggggccg ggtgaggagg aagtcctcgg agaagatgtc
     2101 gtcctgaacc tgttcccagc cggtctcgct cgcctcgacc tggatgcgaa gcagctgctt
     2161 gcggtagttc cactcataga gggccttggc ctcgtcgagc ccctcgtaac actgaaggcg
     2221 gatcagcttg cggccggtcg cgcgagagag agccttggcg agctcggtct tgccgactcc
     2281 agccggccct tcgaccagca ccggcttgcc gagcttggcg gcgaggaagg acacgagtga
     2341 ggcagcctcg tccgcgagat agccgacgtc ggccagcgcc tggcgcgtct cgtcgatgct
     2401 taccggctca ctggtcatgc gcgagaagga tacttttcgg gccatgtggc acctgctccc
     2461 gctgggcgcg tcagaggtac agacctcgcg ctacatcttg ctgctggtga tcggcctgct
     2521 ggtcgcggtg gccgggcaca tctcgcgcac gaagaccctg atcgcgctcg gcctgctgct
     2581 gatcgtggcg gggacgttcc ttttccagtt cgaggtgcgg gcgcagtagc tctccgctcg
     2641 cgccccggcg gctcggcgcg ttgaaagcag cagtaagggg gagagcggaa aaggtctcca
     2701 ggacgacctt taagttgagc accgtatagg tgctcaaaat caatctcgtt cgaggccatc
     2761 cccttagtgc tcgctgtagg cgcttgagcg ctaggcagca acagcggaga ccgccgaaga
     2821 acgacgccgc accgccgacg ccaagaccgc aggcgtccca ttcgccggcg ccatcgtcac
     2881 cggccgccgc gccggcggct ccgccgcctc cgagccgggc agcagatcag cgcgctcgag
     2941 gatggtccgg atcaccaccc gcatctcgag ctccgcaaac gcccccccga tgcaccgccg
     3001 cgtgccgccc ccgaacggga tccacgaata cgtcgcgggc ttgtcgtcca agaaccgctc
     3061 cggccggaac gccagtggct ccggatatag atccgcccgc cggtggatca gctgtaccga
     3121 agcgagcgtc ggcgtccccg gtggaagcac gtggccgttc gtccgcgtct cctcggtagc
     3181 cacccgtccc acctccggca cgacgggccg cacccgcagc gtctcttgca ccaccgcctg
     3241 caggtatgag tcgtcgtcgg aggtgatctc gtcacgcagc ctgtccatca cgtccgggtg
     3301 gcggaagagg gcgtcgaacg cccaagcgag cccggtcgcg gttgtctcgt gaccggccgc
     3361 gagcagcgtc atcagctgat cacgcagctc gtcgtcgctg agtggtgagc cgtcctcgtc
     3421 gcgggccagc gcgagcagcg acaggatgtc ctcgcgctcc tcgaggtcgt cggcgcgccg
     3481 ccgctcctcg atctcggcgt gcagcagctc gtcggcgcgt gccacaagcc tgccgagcgg
     3541 cgtgcgcatc agcagctccg gcgtctgcgc gagcgccagc atcacgttcg cgcggaagcc
     3601 gaccgaccag ttgaggatgt cacggagcac ggtgcgcagc tcgtcctcgc gcggcccgcc
     3661 tgtcgtgccg aacaccgcgc gcagaatcac gtcgagcgtg atcgcctgca tgcgctcgat
     3721 gagcgggaac ggccggccca ccggccagcg cgccatgtcg cgctcggtgg cctcgcgcat
     3781 ggtctcgacg tagccgcgca tgcgctcgcc gtggagcggg ggcagcatca gcttgcgccg
     3841 gcgcaggtgc tctgcgccct cctgcagcag cagcgaacgc gggccgagca gaggccgcag
     3901 cagcacgtcg cgccccttgg ggagtgtgtt cacgcggtcc ttcgaccaga ccgccttcac
     3961 agcatccgga tccgctagca gcaccattcg cccgaccggc cagaggttga tcgtgaaaac
     4021 cggcccgtag cgctcgtacg cgctgtcgag cgagccgaag gggcgcgtca ggtagcgagc
     4081 ggtttgcagc accgccggct cgcgcggccc gtcggggagc gtccccgtca tgtacgggag
     4141 cgtacacgag tgcgccaatc ttcgccgagg ccagcgccgc cgcttcacta ttctgctggg
     4201 ccgttcccgc aacaggagga tgaatggcgg aggttcagtc agggcttgaa ggcgtagtcg
     4261 ccttcgccac tcagatcgcc gagccggaca aggaggggag tgcccttcgc taccgcggcg
     4321 tggacatcga ggacctcgtc ggccgggtcc gcttcgagca cgtctggggc ctccttgtcg
     4381 acggcggcta cctgcccggc ctgccgcccg ccgagccgca tccgctcgcc atccgctccg
     4441 gggacgcccg cgtggacatc cagtcggcgc tcgccatgct cgcgcccgag tggggcctcg
     4501 gccagcttct cgacatctcc gacgagcagg cgcgacggga cctggcgcgc gcgtcggtga
     4561 tggcgctctc gttcgtggcg cagtcggccc gcgggcctgg ccagccgccg atcccgcaga
     4621 gcgaggtcga caaggccgcc acgatcccgg agcgcttcct catccgctgg cgcggcgagg
     4681 ccgaccccga ccacgcgagg gcgatcgacg cctactggat ctccgcggcc gagcacggca
     4741 tgaacgcctc gaccttcacc gcgcgcaccg tcgcctcgac cggtgccgac gccgccgcct
     4801 cactgtccgc ggccgtcggc gcgctgtccg gaccgctgca cggcggcgcg ccctcgcggg
     4861 tgctcgagat gatcggtgcg gtcgaggaga gcggcgaccc agagagctac gtgaagcagg
     4921 tgctcgactc gggcggccgc ctcatgggct tcg
//
LOCUS       DASVXS010000285         9015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_696167, whole genome shotgun sequence.
ACCESSION   DASVXS010000285 DASVXS010000000
VERSION     DASVXS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696167"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF752_16065"
     CDS             complement(<1..518)
                     /locus_tag="VF752_16065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8857110.1"
                     /translation="MPKTVLKSGITIHYQQVGTGPDVVMVHGITGNLAVWHLHIVPML
                     AERFRLLTYDLRGHGYSDTPPTGYSPDDMATDLLELLDALEIERPVVIGHSYGADIAL
                     YVAAREPERLSEVVAIEAALPAMEGVRSHDDWVGWSYWVRALERAGHPVPPEHRSNLR
                     YLIRATVDLPKQ"
     gene            complement(532..2136)
                     /locus_tag="VF752_16070"
     CDS             complement(532..2136)
                     /locus_tag="VF752_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8857111.1"
                     /translation="MTGTLLIGVDGATFTVLDPYMERGVMPTLRELTDRGARSELRSV
                     MPPLTPPAWTSLMTGKHPGQHGVFDFFQKEEPDSIYFSFASSQDVKSATIWTLASAQG
                     RRVISLNFPLMFPAPPVEGCVVPGGMMPWRQLRLGCHPPGLFDRLKALPSFNPREMLD
                     MELEVKAIEGCPDDEYAEWVELHIRRERRWFDVLRHLMQEEPADLVGVLFDGVDKLQH
                     LCWRFIDPECRPAQPSAWEQEMIERCERYFRSLDEMIGDLVELAGPEATVILASDHGF
                     GPTRDVFHVNSWLEDQGYLAWAEDQNGGERRDATQVGFAEVTRHVHALDWTRTLAYAA
                     TPSSQGINIVGRVPGSDDPLPVETRRDIRARLGAALRDVRRPHDGRPLVEEVWTREEA
                     FPGPHADRGPDLSMVLADGGTISILPSDEIVVRREQTRGHHRWEGIFMASGPGIRPGL
                     RAEDVSIVDVAPLVLYRLGLPVPDDMAGRVPTEIFELEEIELRPPRRAPAGQPEPRTV
                     PDGDLTLEPDEEANLMQRLRALGYVE"
     gene            complement(2133..2438)
                     /locus_tag="VF752_16075"
     CDS             complement(2133..2438)
                     /locus_tag="VF752_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8857112.1"
                     /translation="MTQVDPQVLAAVLDMLNELADDWEYDGAIGADTYFIADLGLESL
                     DIVVIGTMIQERYGRLPFAEFLEELGQRPVEERDLTVAGLAAFICEHRQPVLEEVRR"
     gene            complement(2435..7321)
                     /locus_tag="VF752_16080"
     CDS             complement(2435..7321)
                     /locus_tag="VF752_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8857113.1"
                     /translation="MTVRGADGRDRGVAITGMSCLFPGSPGVDAFWRNILGKVDATSD
                     PPPEAWDPEIYYDPDFADTDKTYCKRGGYLGSLASFDPLAHSIPPIAVGGEPDQWLAL
                     QVAHDALADAGASELPAEVRERTAIVLGKGTYLNGGNAIAVQRGLVIGQTLDLIRRLH
                     PEHSDEELEELRREMQRVLPPLGPETVPGLIPNIVVGRIANRLDLMGPAYTVDAACAS
                     SLVAVQLAVRDLVAGECDLALAGGSQVWMPVATLNVFCRLGALSRKEQIRPFDKDADG
                     TLLGEGIGMVVLKRLADAVRDGDRIYAVIRGVGVSSDGRGVSVMAPRVEGEEQALLRA
                     YDDAAVAPESIGLLEAHGTGTPVGDVVEIEALTRVFGERDGELPHCALGTVKSMISHT
                     IPAAGVAGLIKCALALHHRVLPATLNCDEPNPKLGLERTPFYVNTETRPWIHGGTAPR
                     RAGINAFGFGGINAHAVLEEYDDSALADHLPPWDSELFILESETPEGLADEAEELRAA
                     LDGEPAFALTDLAYTLSLALGRSERPLRLAIVADSHDDLHNKLGQAIEKLRTPGCRRI
                     KNVTGIYYAAEPLGRDGKVVLVFPGEGAQYPNMLADLCLHFAEVREVFDRVDRLYADH
                     PRGDLLSDWVFPRPAFTEAEQRRNDERLMQLDIAVESVLTANGAMHALIRRLVRRVDA
                     VVGHSTGEHSAAFATGVLDVDTDDALRAFSQGLYRAYADAEERHDLPRAVLLALGTDG
                     ASGLAMADEAGGDLHLAMDNCPHQSVLVGEPAAAGRAREIAAREGVVCEVLPYDRAVH
                     TPMFTPFAEELRAVFADIPVGDAPEQLWSCTTAGPYPDDAAAIRELLVQHWTNPVRFR
                     ETIEELYEQGARVFVEVGPRGNMTSFIEDILRGRPHCAVASDQRKRSGVTQLNHLVGL
                     LAVHDVDLDVGHLFAQRPAHEIDWRSRSSSEQDEATQRIPLSTAWPMLRLAPEVMERL
                     RSRPEASPVSSNGHATPVEPEPIEPEPIEPEPVVPEPVAHFATQVAESDDEVAAVVDE
                     HLLTMERFLEASEHVMQAYLQGAAPVVVEEARRPLIGTIVSWTPGTELVCRRVFDPAE
                     DRYLLDHTLGRTVSRGDPELTALALMPLAMSLEIVAEAAACLIPDMTVTGLRDVRANR
                     WLAWGEEPQTLEVTARRLAADGSRERVRVELRDTGENGAPASPAVEATVLLGDAFGAP
                     PAPATVPLEDGRPSRWTPDQLYDEAMFHQATWQAVRALDVLSPGGARARLHVLPRSAM
                     LRSTPEPDFVLDPVVLDAAGQVIGFWAADEFERGKVIFPFRLAALELYEPPRPEGEEL
                     TCVAAIERIGDTLLRSDIDVLDSDGRCWMRLESWEDKRFDVPDRFRPLTVPSELSPLS
                     TRWEAPTAPYARRTVACHRLDTRLPADGALWKGVWARRVLSRREREKFDALRLPESRQ
                     LEWLGARTAAKEAVAELLRARHGLDLLPADIEILAGEQGSPQVKATGLGSVDDAPIVS
                     LTHTEGEAVAFAALAPSRSGAVIGIDIEYLRSRPPGFAEAALRPVERRLLERVPADTT
                     DEWLLRCWCAKEAAGKALGVGIAPGAERQSVVSVDPGTEQVVVDVGGRRLQAHTRRDG
                     ELIVATVLSGALGERSDA"
     gene            complement(7318..>9015)
                     /locus_tag="VF752_16085"
     CDS             complement(7318..>9015)
                     /locus_tag="VF752_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8857114.1"
                     /translation="RGITAEVAVSLAERYRCTLLLVGRTLPGEEAQETAGATELPELR
                     QVFIEARRRAGEQVTPALVEEDCRRVLREREVRDNLARLERTGARVEYLACDVRDADA
                     FAAVIDEAYERYGRIDGVIHGAGVIEDRLIRDKQRDSLERVIATKAGGARTLGEKLRP
                     ESLRFLTFFSSVSGRFGNRGQADYAAASEILNKLAQELDRRWPARVQSINWGPWRKTG
                     MVSPEVEREFAKRGVALIPVDVGCRLMDDELRRARKGEVEVVIGAATGVGREQPLLAR
                     AANVTRGDDGSAEVLRTLDLRHDRYLDDHRVDGLPVLPFAVAMELMAEVATTARPGHD
                     VTGLRDIRLLQGVTVADEAGTAMRVAAVPGPSSSEVNVTIGEPGGGRRYYRALVDLGT
                     PASASGTTEPEPQPLPDLAPFPMEVDAAYRELLFHGPLFQGITAIEGMDERGARAVLR
                     PSTPGGCMTQADGLEWLLDPILLDSALQMQVLWARLNWDVTLLPAEIRAHGCAVPAHM
                     RSRDPVRHELRIRPGIQPPLCHADHWFYAADGTLLATLSDVVGVGTQALNRLAAANA"
BASE COUNT         1291 a         3259 c         3122 g         1343 t
ORIGIN      
        1 cactgcttcg gcaggtcgac cgtcgcgcgg atcagataac ggaggttcga gcggtgctcc
       61 ggtggcacgg gatgaccggc acgctcgagc gcgcgcaccc agtaggacca gccgacccag
      121 tcgtcgtggc tgcgcactcc ctccatcgcc gggagcgccg cctctatcgc cacgacctcg
      181 ctcaggcgct ccggctcccg ggcggcgaca tagagcgcga tgtccgctcc gtagctgtgt
      241 ccgatcacca ccggccgctc gatctcgagc gcgtcgagca gctcgagcag gtcggtggcc
      301 atgtcgtcgg ggctgtagcc ggtcggcggc gtatcgctgt agccgtgccc gcgcaggtcg
      361 taggtgagga gccgaaagcg ctcggcgagc atcgggacga tgtggaggtg ccagaccgcc
      421 aggttgcccg tgatgccgtg gaccatcacc acgtccggcc cggtgccgac ctgctggtag
      481 tggatcgtga tgccgctctt caggacggtc tttggcatct gtgcctccgc ctcactccac
      541 gtagccgagc gcccgcagcc gctgcatcag gttggcctcc tcatcgggct cgagcgtgag
      601 gtcgccatcc gggaccgtcc ggggctcggg ctgcccggcg ggtgcgcgcc gcggcggccg
      661 gagctcgatc tcctcgagct cgaagatctc ggtcggaacg cggcccgcca tgtcgtccgg
      721 cacgggcaga ccgagccggt agagcacgag cggagcgacg tcgacgatcg agacgtcttc
      781 cgcgcgcagc ccggggcgga taccgggccc gctcgccatg aagatcccct cccagcggtg
      841 gtgaccgcgc gtctgctcgc gccgcaccac gatctcgtcc gacgggagga tcgagatcgt
      901 gccgccgtcg gcgagcacca tcgacaggtc cgggccgcgg tcggcgtgcg ggccagggaa
      961 ggcctcctcg cgtgtccaca cctcctcgac cagcggccgg ccgtcgtgcg gccggcgcac
     1021 gtcacgcagc gccgccccga gccgcgccct gatgtcacgg cgcgtctcga cgggcagcgg
     1081 atcgtcgctg cccggcacgc ggcccacgat gttgatgccc tggctgctcg gcgtcgccgc
     1141 gtacgcgagc gtgcgcgtcc aatcgagcgc gtgcacgtgc cgcgtcacct cggcgaagcc
     1201 cacttgcgtt gcgtcgcggc gctcgccgcc gttctgatcc tcggcccagg ccaggtatcc
     1261 ctggtcctcg agccacgagt tcacgtggaa cacatcgcgg gtcggcccga acccatggtc
     1321 ggaggcgagg atcaccgtcg cctcgggacc ggcgagctcg acgaggtcgc caatcatctc
     1381 gtcgagcgag cggaagtagc gctcgcaacg ctcgatcatc tcctgctccc acgcggatgg
     1441 ctgcgcgggc cggcactccg ggtcgatgaa gcgccagcag aggtgctgca gcttgtcgac
     1501 gccgtcgaag agcacgccca cgaggtcggc cggctcctct tgcatgaggt ggcggagcac
     1561 gtcgaaccag cgccgctcgc gccggatgtg aagctccacc cactcggcgt actcgtcgtc
     1621 agggcaaccc tcgatcgcct tgacttcgag ctccatgtcg agcatctcgc gcgggttgaa
     1681 gctaggcagc gccttgaggc gatcgaacag gcctggtggg tggcagccga ggcgcagctg
     1741 gcgccacggc atcatgccgc ccggcaccac gcagccctcg acgggcggcg ccgggaacat
     1801 cagcgggaag ttgagcgaga tcacgcgccg gccctgcgcg ctcgccagcg tccagatcgt
     1861 cgcgctcttg acgtcctgcg agctcgcgaa cgagaagtag atgctgtcgg gctcctcctt
     1921 ctggaagaag tcgaagaccc cgtgctgacc cgggtgcttc cccgtcatca gcgacgtcca
     1981 ggcaggcgga gtgagcggcg gcatcaccga gcgaagctcc gagcgcgctc cccggtcggt
     2041 cagctcgcga agcgtgggca tcacgccgcg ctccatgtac gggtccagca ccgtgaacgt
     2101 ggccccgtcc acgccgatca acagcgtccc ggtcatcgtc gcacctcctc gaggactggc
     2161 tggcggtgct cgcagatgaa ggccgcaagc cccgcgacgg tgagatcgcg ctcctcgacc
     2221 gggcgctggc cgagctcttc gaggaactcg gcgaagggca ggcgcccgta gcgctcctgg
     2281 atcatcgtcc cgatcacgac gatgtcgagc gactcgaggc cgaggtcggc gatgaagtac
     2341 gtgtcggccc cgatcgcacc gtcgtactcc cagtcgtcgg cgagctcgtt gagcatgtcg
     2401 agcacggctg cgagcacctg tggatcgacc tgggtcatgc gtcgctcctc tctccaaggg
     2461 cgcccgacag gacagtcgcg acgatcagct ccccgtcgcg ccgcgtgtgc gcctgcagcc
     2521 gccgtccgcc gacgtccacg accacctgct cggtgccggg gtcgacggag acgaccgact
     2581 gtcgttctgc accaggcgcg atgcccactc cgagcgcctt gcccgccgcc tccttcgcgc
     2641 accagcagcg cagcagccac tcgtccgtgg tgtcggcggg gacgcgttcg agcagccgcc
     2701 gctccaccgg cctgagcgcc gcttcggcga agcccggtgg gcgcgacctc agatactcga
     2761 tgtcgatacc gattaccgcg ccgctccgcg atggagcgag cgcggcgaag gccacggcct
     2821 cgccctctgt gtgagtgagc gagacgatcg gggcgtcgtc gacgctcccg aggccggtgg
     2881 ccttcacctg gggcgaaccc tgttcgccgg ccaggatctc gatgtcagcc ggcaacaggt
     2941 caagcccgtg ccgagctcgg agcagctccg ccacagcctc cttcgcggcg gtgcgcgcgc
     3001 cgagccattc gagctgccgg ctctccggca gccgcaatgc gtcgaacttc tcccgctcac
     3061 ggcgactgag tacgcggcgg gcccacacgc ccttccagag cgcgccgtcg gccggcagtc
     3121 gtgtgtccaa ccggtggcag gccacggtgc ggcgcgcgta gggagcggtc ggcgcctccc
     3181 acctggtcga gagcggtgag agctcggacg gaaccgtgag cggcctgaag cggtctggga
     3241 cgtcgaagcg cttgtcctcc cagctctcga gccgcatcca gcatcggccg tcggagtcga
     3301 gcacgtcgat gtccgagcgc agcagcgtgt cgccgatccg ctcgatcgcc gcgacacacg
     3361 tcagttcctc gccctccggc cgcggcggct cgtagagctc cagcgccgcg agccggaacg
     3421 ggaagatcac cttgccgcgc tcgaactcgt cggcggccca gaatccgatc acctgcccgg
     3481 ccgcatcgag caccaccggg tcgaggacga agtccggttc gggcgtgctg cgcagcatgg
     3541 ccgagcgcgg tagaacgtgc aggcgggcgc gcgcaccgcc cggggagagc acgtctagcg
     3601 cgcgcaccgc ctgccacgtg gcttgatgga acatcgcttc gtcgtagagc tggtccggcg
     3661 tccagcgcga cggcctgccg tcctcgagcg gcaccgtggc aggcgcgggc ggggcgccga
     3721 aggcgtcgcc cagcaacacc gtcgcctcca cggccgggct cgccggcgcg ccgttctcac
     3781 cggtgtctcg cagctccacc cgcacgcgct cgcggcttcc atcagccgcg agccggcgcg
     3841 cggtcacctc cagcgtttgc ggctcctcgc cccaagccag ccagcggttc gcgcgaacgt
     3901 cgcggaggcc ggtcacggtc atgtcgggga tcaggcatgc cgcggcctcg gccacgatct
     3961 ccaggctcat cgcaagcggc atgagcgcca gcgcggtcag ctcggggtcg ccccgcgaaa
     4021 ccgtccggcc gagcgtgtgg tcgagcaggt aacggtcctc ggccgggtcg aagacgcgcc
     4081 ggcacacgag ctcggttccc ggcgtccagg agacgatcgt tccaatgagg ggccggcgcg
     4141 cctcctcgac gactaccggc gcagcgccct gaagataggc ctgcatcacg tgctcgctcg
     4201 cctcgaggaa gcgctccatc gtgagcaggt gctcgtccac cacggccgcg acctcgtcgt
     4261 cgctctcggc gacctgcgtt gcgaagtgcg ctaccggctc gggcacgacc ggttcgggct
     4321 cgatcggctc gggctcgatc ggctcgggct cgaccggggt tgcgtgaccg ttgctggaaa
     4381 ccggcgaagc ttccgggcgc gagcgcagcc gctccatcac ctcgggcgcg agccgcagca
     4441 tgggccacgc ggtcgagagc gggatcctct gcgtggcttc gtcctgctcc gacgaggatc
     4501 gggagcgcca gtcgatctcg tgggcgggcc gctgcgcaaa cagatgtccg acatcgaggt
     4561 ccacgtcgtg gacggccagc agcccgacca ggtggttgag ctgggtcacg ccggaccgct
     4621 tccgctggtc ggaggcgacc gcgcagtgcg gacgcccgcg caggatgtcc tcgatgaacg
     4681 aggtcatgtt cccgcgcggc ccaacctcga caaacacgcg cgcgccctgc tcgtacagct
     4741 cctcgatcgt ctcgcggaag cgaacggggt tggtccagtg ctgcacgagc agctcgcgga
     4801 tggcggcggc gtcgtcggga tacggccccg cggtggtgca tgaccagagc tgctccggcg
     4861 cgtccccgac ggggatgtcc gcgaacaccg cgcgcagctc ttccgcgaac ggcgtgaaca
     4921 tcggcgtgtg caccgctcgg tcgtagggca gtacctcgca gacgacgccc tcacgcgcgg
     4981 cgatctcccg cgcgcggccc gccgccgcag gctcgcccac gaggaccgac tggtgcgggc
     5041 agttatccat ggcgagatgg aggtcgccgc ccgcctcatc ggccatcgcg agccccgacg
     5101 ccccgtcggt gcccagcgca agcaggaccg cgcgcgggag atcgtggcgc tcctccgcgt
     5161 cggcataggc gcgatagaga ccctgcgaga acgctctcag cgcgtcgtcg gtgtcgacgt
     5221 cgagcacgcc ggtcgcgaac gccgccgagt gctcacccgt gctgtggccc accaccgcat
     5281 cgacgcgccg gacgaggcgg cgtatcagcg cgtgcatcgc gccgttggct gtcagcacgg
     5341 actcgaccgc gatgtcgagc tgcatcagcc gctcgtcgtt gcgccgctgc tccgcctcgg
     5401 tgaacgccgg ccgcgggaag acccagtcgc tcagtagatc gccgcgcgga tgatcggcgt
     5461 agaggcggtc gacccggtcg aagacctcgc gtacctcggc gaagtgcagg cagaggtcag
     5521 cgagcatgtt cgggtactgc gctccctcgc cggggaacac gagcacgacc ttgccgtcgc
     5581 gcccgagcgg ctccgctgcg tagtagatgc ccgtgacgtt cttgatccgg cggcagccgg
     5641 gggtgcgcag cttctcgatc gcctgaccga gcttgttgtg gaggtcgtcg tgcgagtccg
     5701 cgacgatcgc gagccgcaga ggccgctccg agcggcctag cgcgaggctg agcgtgtacg
     5761 cgagatccgt gagggcgaac gccggctcgc catccagcgc cgccctcagc tcctcggcct
     5821 cgtccgcgag gccctcaggt gtctcgctct ccagaatgaa gagctcgctg tcccacggcg
     5881 gcaggtggtc ggcgagggcc gagtcgtcgt actcctcgag caccgcgtgc gcgttgatcc
     5941 cgccgaagcc gaacgcattg atgccggcgc gccgcggcgc cgtgccgccg tggatccagg
     6001 gtcgcgtctc ggtgttgacg tagaacggcg tccgctcgag cccgagcttc gggttcggct
     6061 cgtcgcagtt cagcgtcgcc ggcagcacgc gatggtggag cgcgagcgcg cacttgatca
     6121 gcccggccac gcccgccgcg ggaatggtgt ggctgatcat cgacttcacc gtgccgagcg
     6181 cgcagtgcgg cagctcgccg tcgcgttcgc cgaagacgcg cgtcagcgcc tcgatttcca
     6241 ctacgtcgcc cactggcgtc cccgtgccgt gggcctcgag cagcccgatg ctctccggcg
     6301 caacggcggc gtcgtcgtat gcgcgcagca acgcctgttc ctccccctcc acgcgcggtg
     6361 ccatgacgct caccccgcgg ccgtcgctcg acacgcccac gccacggatc accgcgtaga
     6421 tgcggtcgcc gtcgcggacg gcgtcggcga gccgcttcag cacgaccatg ccgatgccct
     6481 cgccgagcag agtgccgtca gcgtccttgt cgaacggccg gatctgctct ttgcgcgaga
     6541 gcgcgccgag ccggcagaac acgttcagcg tcgccaccgg catccacacc tgcgagccgc
     6601 ccgcgagcgc caggtcgcac tcgcccgcga ccaggtcgcg taccgcgagc tgcacggcca
     6661 cgagcgacga ggcgcacgcc gcatcgaccg tgtacgcggg gcccatcagg tcgagccggt
     6721 tcgcgatgcg cccgacgacg atgttcggga tcagccctgg aaccgtctcg ggcccgagcg
     6781 gcggcaggac ccgctgcatc tcccggcgca gttcctcgag ctcctcgtcg gagtgctcgg
     6841 gatgcaggcg ccggatgagg tcgagggtct ggccgatcac caagccacgc tgcacggcga
     6901 tcgcgttgcc gccgttgagg tacgtgccct tgccgaggac gatcgcggtg cgctcgcgga
     6961 cttccgccgg cagctcgctg gctccagcgt cggcgagggc gtcgtgcgcg acctggaggg
     7021 cgagccactg atcgggttcc ccgccgaccg ctatcggcgg gatgctgtgg gcgagcggat
     7081 cgaaagacgc aagcgagccg agatagccgc cgcgcttgca gtacgtcttg tccgtatccg
     7141 cgaagtccgg gtcgtagtag atctcggggt cccaggcctc cggcggcgga tccgaggtcg
     7201 cgtcgacctt gccgaggatg ttgcgccaga aggcgtcgac gccgggtgag cccgggaaca
     7261 ggcacgacat cccggtgatg gcgacgccgc ggtcgcggcc gtctgcgccc cgcaccgtca
     7321 tgcgttcgcc gccgcgagcc ggttgagtgc ctgcgttccg acgcccacca cgtcgctgag
     7381 cgtcgccagc agtgtgccgt cggctgcgta gaaccagtgg tcggcgtggc agagcggcgg
     7441 ctggatcccc ggccggatcc tgagctcgtg ccggaccggg tcacgtgagc gcatgtgggc
     7501 ggggaccgcg cagccatgcg cgcggatctc ggcgggcagc agcgtcacgt cccagttgag
     7561 ccgcgcccac agcacctgca tctgcagggc gctgtcgagc aggatcggat cgagaagcca
     7621 ctcgaggccg tccgcctgcg tcatgcagcc tccgggcgtc gatggccgca gcacggctcg
     7681 agcgccgcgc tcgtccatac cctctatcgc cgtgatgccc tggaacagcg ggccgtggaa
     7741 gagcagttcg cggtaggcgg catccacttc catcgggaac ggcgcgaggt cgggcagcgg
     7801 ctggggttcc ggctcggttg tgccgctcgc gctagccggc gtgccgagat cgaccagcgc
     7861 gcggtaatag cggcggccgc cgccgggctc gccgatcgtc acgttcacct cagagctcga
     7921 cggtccgggt acggcggcaa cccgcatggc cgtaccggcc tcgtcggcga ccgtgactcc
     7981 ctgcagcagg cggatgtcgc ggagcccggt cacgtcgtgc cccgggcgcg cggtcgtcgc
     8041 cacctccgcc atcagctcca tggccacggc gaacggcagc accggcaggc cgtctacgcg
     8101 atggtcatcg aggtaacggt cgtgtcgcag atcgagggtt cgcagcacct cggcgctgcc
     8161 gtcgtcgccg cgcgtcacgt tcgctgcgcg cgcgagcagt ggctgctcgc gccctactcc
     8221 ggtggccgca ccgatcacga cctcgacttc gcccttgcgc gcgcgacgca gttcgtcgtc
     8281 catcagccgg cagccaacat ccacggggat cagcgccacg ccgcgcttgg cgaattcgcg
     8341 ctcgacctcc ggcgagacca tcccggtctt ccgccacggg ccccagttga tcgattgcac
     8401 gcgagcgggc cagcgccggt cgagctcctg agcaagcttg ttgaggatct cgctcgccgc
     8461 ggcgtaatcg gcctggcccc ggttgccgaa ccggcccgag acagagctga agaaggtcag
     8521 gaagcgaagg gactccggcc gcagcttctc ccccagcgtg cgcgcgccgc cggccttcgt
     8581 cgcgatcacg cgctcgagag agtcgcgctg cttgtcgcgg atcaggcggt cctcgatcac
     8641 tccagcgccg tggatcacgc cgtcgatccg gccgtaccgc tcgtacgcct cgtcgatcac
     8701 cgccgcaaac gcgtccgcgt cgcgcacgtc gcaggcgagg tactccacgc gcgcgcccgt
     8761 gcgctcgagc cgcgcgaggt tgtcgcgtac ctcgcgctcg cgcagcactc ggcggcaatc
     8821 ctcctcgacg agcgccggcg tgacctgctc gccggcgcgg cggcgcgcct cgatgaacac
     8881 ctgccgaagc tctgggagct ccgttgcccc ggcagtctcc tgtgcctctt cgccggggag
     8941 cgtgcggcca accagcagca acgtgcagcg gtagcgctcg gcgaggctaa cggcgacctc
     9001 ggcggtgatc cctcg
//
LOCUS       DASVXS010000286         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1263265, whole genome shotgun sequence.
ACCESSION   DASVXS010000286 DASVXS010000000
VERSION     DASVXS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263265"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1118)
                     /locus_tag="VF752_16090"
     CDS             complement(198..1118)
                     /locus_tag="VF752_16090"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX8857115.1"
                     /translation="MPTAWPAIPYDDWRATCDTLHAHTQVLGKLAVALAPPEPQLQHA
                     ALQLTARGWETLPLPAPDGSGAIVVALDLRTHEAVVEHSSGREQRVALTPNRPVGDVT
                     REVIERLSSLAGEVEINATPQEVWWTVPLDEDHEHATYDPAQVAPYFAAASQAALVLA
                     EFRAAYRGRKTPVNAWWGSFDLAVSLFSGEWADPPSDDFITRNSMDVQEVATGWWPGD
                     PRFGEAAFYSYAHPAPEGFKDAKLSPSAARWHEELGLFVLDWDDVRASPDPHTTALEF
                     AHSAFRHACMVCEWEPALLASAEGSPPPVN"
     gene            complement(1129..1710)
                     /locus_tag="VF752_16095"
     CDS             complement(1129..1710)
                     /locus_tag="VF752_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8857116.1"
                     /translation="MPAATKDRIIDTTAELFRRYGYTGTGMKQIVAEAQAPFGSLYHF
                     FPGGKEQLGQETIRRSGQMYRELFEAIADPAPDIVTGVRSFFEGAAAVLEETDYADAC
                     PIATVALEVASTSEPLREATADVFNSWIDAATARLEAEGASPSGAREAAISMIASLEG
                     AFILSRAFKSTEPMHVAGEHAVAVVRRALTREE"
     gene            1763..2599
                     /locus_tag="VF752_16100"
     CDS             1763..2599
                     /locus_tag="VF752_16100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8857117.1"
                     /translation="MAEIQLSAGTIEYEDTGEGPLLVLIHGATMNGSQWSDVVSELQP
                     DHRCVVPTLPLGGHRIPMNEDADLSLRGQVRILEDFLEQLDLRQLTLVGNDWGGPQLL
                     SRNDRVARLVLTSCEAFDNFPPGLPGKNLALTGRMPGGFALAARALRVRALRRTPITY
                     GWMSKRPLPDEVMDEWLRPLMTDPRIRRDLAKYVHPLERDAFLKAAEELRSFDKPALV
                     VWAKEDRVMPPEHGRRLAELLPQGRLVEIEDSYTLIPQDQPLALARALREFVHETAPV
                     TA"
     gene            2596..3093
                     /locus_tag="VF752_16105"
     CDS             2596..3093
                     /locus_tag="VF752_16105"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8857118.1"
                     /translation="MRRTAAVALLSLPAAQATYAWLVRRLFMRNLRNLRAGNPEPLFK
                     SYADDVRFVFPGDSSWGGEVRGREALEHWVRRFVRVGLQLEPQKILITGPPWKTTFCL
                     LYTDELTTPSGERVYENRGAIYGTIRWGKITSYETAEDTHKVEAFDEWLAEHEQELSA
                     SSAAA"
     gene            complement(3068..>3586)
                     /locus_tag="VF752_16110"
     CDS             complement(3068..>3586)
                     /locus_tag="VF752_16110"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX8857119.1"
                     /translation="SDDATTVQRSAAQKAREGEKSPQAAVSQQAKAGANGERKVNAKV
                     VHGPHGSVAVAVPVYVNGEGPFAFILDTGASQSVIDTALARELKLEKTGSKAPVQGVA
                     GSTVAREVVIEKWRVDKMPLRTLAGVAVGLASGDKHAGVAGLLGSDVLSGFESVAIDY
                     QKQVVTLRPKRR"
BASE COUNT          541 a         1276 c         1210 g          559 t
ORIGIN      
        1 gcaaccagac gcggcaccag ctaccgcccc atcgctcccg tcgcgcctgc gacaaacact
       61 ctcatcatca actccctgac cgcgattacc ttcaccccag aagagaagac ggcagcccag
      121 gctgtgacac ctccgcgcac cccagcacgg gaacgccggc acgcgggcac ctgatcgtcg
      181 cctgctcggg gcgtctctca gttgaccggc ggcggcgaac cttcggcgct cgccagtagc
      241 gccggctccc actcgcagac catgcacgcg tggcggaagg cggagtgtgc gaactcgagc
      301 gcagtcgtgt gcgggtcggg gctcgcccgg acatcgtccc aatcgagcac gaacaggccg
      361 agttcctcgt gccagcgtgc cgccgacggc gagagcttcg cgtccttgaa gccctcgggg
      421 gccgggtgcg cgtaggagta gaacgccgcc tcaccgaacc tcgggtcgcc cggccaccag
      481 ccggtggcga cctcctgcac gtccatcgag ttgcgcgtga tgaagtcgtc ggaaggcgga
      541 tccgcccact cgcccgagaa gaggctcacg gcaaggtcga acgatcccca ccaggcgttc
      601 acgggcgtct tccggccgcg gtacgccgcc cggaactcgg cgaggacgag cgcggcctgc
      661 gaggcggcag cgaagtacgg cgcgacctga gcagggtcgt acgtcgcgtg ctcgtggtcc
      721 tcgtcgagcg gaacggtcca ccacacttct tgcggcgtcg cattgatctc cacctcaccg
      781 gcgaggctgc tcagccgttc gatcacctcg cgcgtgacgt cgcctacggg tcggttcggg
      841 gtgagcgcca ctcgctgctc ccgcccgctg ctgtgctcga ccacggcctc gtgcgtcctg
      901 aggtcgagcg cgaccacgat cgcgccggag ccgtcgggcg ccggtagggg gagcgtctcc
      961 cagccgcgag cagtgagctg gagtgccgcg tgctgcagct gcggctcagg tggagcgagc
     1021 gccaccgcaa gcttgccgag cacctgggtg tgcgcgtgca gcgtgtcgca agtggctcgc
     1081 caatcgtcgt acgggatggc gggccatgcc gtgggcatgg tcttcaagct actcctctcg
     1141 ggtcagcgcc ctgcgaacga cggcgaccgc gtgctctccc gccacgtgca tcggctcggt
     1201 gctcttgaag gctcggctga ggatgaaggc gccctcgagc gaggcgatca tcgagatcgc
     1261 cgcctcgcgc gcgccgctcg ggctcgcgcc ctcggcttcg agccgagccg tcgcggcgtc
     1321 gatccagctg ttgaacacgt cggcagtcgc ctcgcgcagc ggctcgctcg tgctcgctac
     1381 ctcgagcgcg acggtcgcga tcgggcaggc gtcggcgtag tcggtctcct cgagaaccgc
     1441 agcggcgcct tcgaagaagc ttcgaacacc ggtcacgatg tcgggcgccg ggtcggcgat
     1501 cgcctcgaac agctcgcggt acatctggcc ggagcggcgg atcgtctcct ggccgagctg
     1561 ctccttgccg cccgggaaga agtggtagag cgacccgaag ggagcctgcg cctcggccac
     1621 gatctgcttc atgccggtgc cggtgtagcc gtagcgtcgg aacagctcgg cggtggtatc
     1681 gatgatgcgg tccttcgtgg cggcgggcat tgcggtacct taccgctaga gcgatctatc
     1741 tagtcccctg ggaggccgag ccatggccga gattcagctg tccgcaggca cgatcgagta
     1801 cgaggacacc ggcgagggac ccctgctcgt gctgattcac ggcgcgacca tgaacggatc
     1861 gcagtggagc gatgtcgtga gcgagctgca accggatcac cgctgcgtgg tgccaacgct
     1921 gccgctcggc ggacaccgca tcccgatgaa cgaggacgcc gatctgtcgc tgcgcggaca
     1981 ggtccggatc ctggaggact tcctcgagca gctcgacctg cggcagctca cgctcgtcgg
     2041 caacgactgg ggcggcccgc agttgctctc gcggaacgac cgcgtggcac ggctcgtgct
     2101 cacgtcgtgc gaggccttcg acaacttccc gccaggcctg ccaggcaaga acctcgcgct
     2161 caccggacgc atgcccggcg gcttcgcgct ggccgcacgc gcactcaggg tgcgcgccct
     2221 gcgccgcaca ccgatcacgt acggctggat gtcgaagcgg cccttgccgg acgaggtgat
     2281 ggacgagtgg ctgcgcccgc tcatgaccga ccctcgcatc cgccgcgacc tcgccaagta
     2341 cgtgcaccca ctcgagcgcg acgcgttctt gaaggcggct gaggagctgc gctcgttcga
     2401 caagcccgcg ctcgtcgtgt gggcgaagga ggaccgcgtg atgccgcccg agcacggccg
     2461 ccggctcgcc gagctgctgc cacaggggcg gctcgtggag atcgaggaca gctacacgct
     2521 gatccctcag gaccagccgc tcgcgctggc ccgggcgctt cgcgagttcg tgcacgagac
     2581 ggccccggtg acggcatgag gcgcacagcc gcggtcgcgc tgctgagcct gcccgctgcg
     2641 caggccacct acgcgtggct cgtgcgccgc ctcttcatgc gcaaccttcg caacttgaga
     2701 gccggcaacc ccgagccact gttcaagtcg tacgccgacg acgtccgctt cgtgttcccg
     2761 ggcgacagct cgtggggcgg cgaggtgcgc ggccgggagg cgctcgagca ttgggtgcgc
     2821 cgcttcgtgc gcgtcgggct gcagctcgag ccgcagaaga tcctcatcac cggcccgcct
     2881 tggaagacca cgttctgcct gctctacacc gacgagctca ccaccccgag cggcgagcgc
     2941 gtctacgaga accggggcgc gatctacggg acgatccgct ggggcaagat cacctcgtac
     3001 gagaccgccg aggacacgca caaggtggag gcgttcgacg agtggctcgc cgagcacgag
     3061 caggagctca gcgcctcttc ggccgcagcg tgaccacctg cttctggtag tcgatcgcga
     3121 ccgactcgaa gccgctgagc acatccgacc cgagcaggcc cgcgacgccc gcgtgcttgt
     3181 ccccgctcgc caatccgacg gccacgccgg cgagcgtgcg cagcggcatc ttgtcgaccc
     3241 gccacttctc gatcacgacc tcgcgcgcga cggtgctgcc tgccacgccc tggaccggcg
     3301 ccttgctgcc ggtcttctcg agcttcagct cacgcgccag cgcggtgtcg atcaccgatt
     3361 gggaagcgcc cgtgtcgagg atgaatgcga acggcccctc gccgttcacg taaaccggta
     3421 cggcgacggc caccgagccg tgtgggccgt gcacgacctt ggcgttgacc ttgcgctcgc
     3481 cgttcgcgcc ggccttcgcc tgctgggaga ccgccgcctg cggtgacttc tctccctcgc
     3541 gcgccttttg ggcggcgctc cgctgcacag tggtggcatc gtcgct
//
LOCUS       DASVXS010000287         8141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_768961, whole genome shotgun sequence.
ACCESSION   DASVXS010000287 DASVXS010000000
VERSION     DASVXS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768961"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2202)
                     /locus_tag="VF752_16115"
     CDS             complement(<1..2202)
                     /locus_tag="VF752_16115"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8857120.1"
                     /translation="MRRWTRAIIRNRKKVLLAWLAVFVLGAAGAANLGGLLTNRFSVP
                     GSEAEKGLDVAKTHFGEHGDGNFTLVLQSTGGSPRNPEFVRSAEEAAQRAASAIKGGK
                     AGPVLQAGDSIAYVQVTTPLENADASDKTNDVRKALGTVPGARAYVSGFPAINHDTQK
                     IYNEDLARGESIAIPVALIVMLFMFGTLAAISVPFAFALVTIPTTLGIVFIFAHFMDM
                     AIYVTNIVTLIGFAIAIDYSMLVVFRYREELERHDHPHAALERTMLTAGRATIFSGLT
                     VAIGLALLILMPLPFMRSMGVGGVMVPLVSIAASATLLPALLAVMGRRVNRLRIIPRR
                     ILKSRAQAKAGFWTKLANSIMRRPVPYLVIGTALMLALAAPAITLSLTGGDNRGVPKT
                     TEATRGLALLEGTLGPGALAPHQIVVDAGRPGAAWSAQQIAAQRRFIAAVSRDPEVQP
                     RTIQAPAAVIRTPGAPSPAVRAALERASIMDADGSAYQIRVAGKHDAGTHAAMDLVHR
                     LRDRYVPQAGFATTVYVSGAPAFGVDFIDKAYGAFPWLVAGVLVISYLLLMRAFRSLF
                     LPAKAVFMNLLSVSATYGVLVLAFQHGWGNVVGWQSSPQIEAWIPIFLFAILFGLSMD
                     YEVFLLSRMREEWDRRHNNEQAVARGLEHTGRIITAAAIIMVAAFSGFTAGSFVGLQQ
                     FGLGLSAAVLLDATIVRAILVPATMKLVGEWNWYLPESLRKLMRLRPAPVPV"
     gene            2327..2716
                     /locus_tag="VF752_16120"
     CDS             2327..2716
                     /locus_tag="VF752_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857121.1"
                     /translation="MGFDQYHEPPEELPARARTFARLCASLTEEAEAIGWYEQRLAVE
                     PEPEARAIMSDAQGEEFKHFSMDLEALLRRTPAWREIAEGILFQPGDIVEHGEEAEAE
                     TLEAGPETSTPGEPSGSLGIGSLRGVA"
     gene            2716..3522
                     /locus_tag="VF752_16125"
     CDS             2716..3522
                     /locus_tag="VF752_16125"
                     /inference="COORDINATES: protein motif:HMM:NF016349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX8857122.1"
                     /translation="MNHLLRSHAPISDAGWELLDNEAKERLSAALAARRLVDFSGPYG
                     WERSSSNIGRSQPLPDALCEGVSARQRRVLPLIELRSEFTVSREELRDADRGAPDADL
                     GSLDRATRQIALAENIAVFHGFQAAGMAGVADASPHAPITLSDDFDDYPGHVAKAVEL
                     LLEVGVDGPYGLALGPDGYTGVIESTEHGGYLVFDHLRKILDGPVVYAPGVRGAVVLS
                     TRGGDFLFESGQDLSIGYDHHDAGSVHLYLEESFSFFVATPDAAVSLTAA"
     gene            complement(3505..3864)
                     /locus_tag="VF752_16130"
     CDS             complement(3505..3864)
                     /locus_tag="VF752_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857123.1"
                     /translation="MGKTWVLDTETKGTGAQMVPIEKKLNGGAGGSGPVVVEPRRRRE
                     PEPAKQAEPRTFRVVDAMSRQVIADGTDLRETIRALEGARSVVDVSVWVWDEAQDDWR
                     LLTFGERQALWRLRGSE"
     gene            3948..4481
                     /locus_tag="VF752_16135"
     CDS             3948..4481
                     /locus_tag="VF752_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857124.1"
                     /translation="MAARAALFGVLAALAWPAGALAVIGDTSGGTAPPAGAPAPTTVA
                     AVTLTAGVARTSATAPRAVQLAVRAGNRLKHKAYRYGGGHKSFKDAAYDCSGTVSYVL
                     HAAKLLASPLDSSGLMTWGAPGPGSWITVYANASHAFMVVGGLRLDTSGPGPSGPRWR
                     ALPRSLDGFTARHPLGL"
     gene            complement(4700..5377)
                     /locus_tag="VF752_16140"
     CDS             complement(4700..5377)
                     /locus_tag="VF752_16140"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8857125.1"
                     /translation="MPAGLPLASLTNTLTDWIGNHGVYAVFTIMAVDALLPLGGELTM
                     LYAGVLASGAIAGQAAVLFGAHLSSGIESYIVLALAGSLGYLAGSLIGWVIGNRGGRP
                     FVERHGRWLHLPPDRFGRAERWFDSHESLAVFLGRLTPLVRSFISIPAGVFGTPIARY
                     VGLTLAASLIWCFAFAGAGWALGGSWESFHHDFKYADYAAVLAVVALAVGAWAVQRRQ
                     GAEARNL"
     gene            5730..5924
                     /locus_tag="VF752_16145"
     CDS             5730..5924
                     /locus_tag="VF752_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857126.1"
                     /translation="MADHKEELLQLIEGSPNPPLMLGGMAETIGVSYEEAERLLEELV
                     REGRVRMEGETPVAVEPPDR"
     gene            5929..6327
                     /locus_tag="VF752_16150"
     CDS             5929..6327
                     /locus_tag="VF752_16150"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX8857127.1"
                     /translation="MATALFVCLHNAGRSQMSQALFERAAAGRHRALSAGTTPGDRVH
                     PEVVDAMHELGIDLSGNVPRKLTRELAEEADVVVTMGCGDECPYIPGKRYIDWDLEDP
                     KGRPLHEVRETRDEIERRVRELVAELDGSA"
     gene            complement(6370..6549)
                     /locus_tag="VF752_16155"
     CDS             complement(6370..6549)
                     /locus_tag="VF752_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857128.1"
                     /translation="MCHSRRWYEKRDESFERELRDLYDRARERPEKVTPVVEKESADE
                     PTAEPEREDAPVLRS"
     gene            complement(6599..7435)
                     /locus_tag="VF752_16160"
     CDS             complement(6599..7435)
                     /locus_tag="VF752_16160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8857129.1"
                     /translation="MADRTVLITGTSTGIGRATAAELAARGFRVLAGVRREEDAEAVR
                     ALDPARIEPLMLDVTDFDAIASLPDRVDGDLAGVVNNAGMALPGPLEYLPLEDVRRQL
                     DVMLVAPFALTKAFIPALRRSRGRLVIIGSIGGRMAGPFISPYNAAKFGVEGLADSLR
                     RELTPSGIEVSLVEPGSVKTEIWEKGIRAGNELFDSLPEEGRRLYGDRIRATEKVATD
                     AVRRGVKPEKVARAVLHALTADRPRTRYLVGIDARVQATLAAVLPDRMLDRTMSRLTG
                     VR"
     regulatory      7539..7685
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            7720..>8141
                     /locus_tag="VF752_16165"
     CDS             7720..>8141
                     /locus_tag="VF752_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857130.1"
                     /translation="MLALAALSLTAAAPASAKPRTPAPKISSVRCWPVSACTSDPHTV
                     RAGGRLRFRGRNLKHGMQVRFQGTVIAGKKTALVAKLRLRRPDGFVATVPTTARSGRV
                     RVVSPTGRRSNLAGPITIVSTPVKGTGTALDGNGVWIW"
BASE COUNT         1304 a         2808 c         2835 g         1194 t
ORIGIN      
        1 gaccgggacc ggcgccggtc tcagccgcat cagcttccgc aggctctcgg ggaggtacca
       61 gttccactcg ccaacgagtt tcatggttgc gggcacaagg atcgcgcgta cgatcgtcgc
      121 gtcgagcagc acggccgccg agaggccgag gccgaactgc tgcaggccca cgaagctgcc
      181 cgccgtgaag cccgagaacg ccgccaccat gatgatcgcg gccgcggtga tgatgcggcc
      241 ggtgtgctcg agcccgcgcg cgaccgcctg ctcgttgttg tggcggcggt cccactcctc
      301 gcgcatgcga gacagcagga acacctcgta gtccatcgag agcccgaaca ggatcgcgaa
      361 caggaagatc gggatccacg cctcgatctg cggcgagctc tgccacccga ccacgttgcc
      421 ccagccgtgc tggaaggcga gcacgagcac gccgtaggtg gcgctcaccg acaggaggtt
      481 catgaacacc gcctttgccg gcaggaacag cgagcggaag gcgcgcatca gcagcagata
      541 gctgatcaca agcactccgg cgacgagcca cgggaacgcg ccgtacgcct tgtcgatgaa
      601 gtcgacgccg aaggcgggcg caccgctcac gtacacagtc gtcgcgaatc cggcctgcgg
      661 cacgtaccgg tctcgcagcc ggtgcacgag gtccatcgcg gcgtgggtgc cggcgtcgtg
      721 cttccccgcc acacggatct ggtacgcgct gccgtcggcg tccatgatgg aggcgcgctc
      781 gagcgccgcc cgcaccgctg ggctgggggc gcctggggtg cggatgacgg ccgcgggcgc
      841 ctggatcgtg cgcggctgca cctctggatc gcgcgacacc gccgcgatga agcggcgctg
      901 tgcggcgatc tgctgggcgc tccaggcggc gcccggcctg ccagcgtcga ccacgatctg
      961 gtgcggcgcg agcgcacccg gtccgagcgt gccctcgagc agcgcgaggc ctctcgtcgc
     1021 ctccgttgtc ttcggcacac cgcggttgtc gccgccggtc aggctcagcg tgatcgccgg
     1081 ggccgcgagc gcgagcatca acgcggtccc gatcacgaga tacggaaccg ggcgccgcat
     1141 gatcgagttc gcgagcttcg tccagaagcc ggccttcgcc tgagcgcggc tcttcaggat
     1201 gcggcgcggg atgatgcgta ggcggttcac ccggcggccc atcactgcga gcagcgcggg
     1261 gaggagtgtg gccgaagccg cgatcgacac gagcgggacc atcaccccgc ccacgcccat
     1321 ggagcgcatg aatgggagcg gcatgaggat cagcagtgcg agcccgatcg cgaccgtcag
     1381 cccggagaag atcgtggcgc gccccgcggt gagcatcgtg cgttccagcg ccgcgtgggg
     1441 gtggtcgtgg cgctcgagct cctcgcggta gcggaagacc acgagcatcg agtagtcgat
     1501 ggcgatcgcg aagccgatca gcgtgacgat gttcgtcacg tagatggcca tgtccatgaa
     1561 gtgggcgaag atgaacacga tcccgagggt ggtcgggatg gtcaccagcg cgaacgcgaa
     1621 cggcaccgag atcgccgcga gcgtgccgaa catgaacagc atcacgatca gcgcgaccgg
     1681 gatggcgatc gactcacccc gcgcgaggtc ctcgttgtag atcttctgcg tgtcgtggtt
     1741 gatggccggg aagcccgaca cgtaggcgcg tgcgcccggg accgtgccga gcgccttccg
     1801 gacgtcgttc gtcttgtccg atgcgtccgc gttctcgagc ggtgtcgtta cctgcacgta
     1861 ggcgatgctg tcgccggcct gcaggaccgg tccggccttg cctcccttga tcgctgacgc
     1921 ggcgcgttgc gcggcttctt ccgccgagcg cacgaactcg gggttccgtg gcgagccgcc
     1981 agtgctctgc aagaccagcg tgaagttgcc gtcgccgtgc tcgccgaaat gcgtcttcgc
     2041 gacgtcgagg cccttctccg cctccgagcc cggcacgctg aaccgattgg tcaggaggcc
     2101 tccgaggttg gccgcgccgg ccgcgcccag cacgaacacg gccagccacg cgaggaggac
     2161 cttcttcctg ttgcggatga tggcgcgagt ccaacgccgc acgacgcaag cctaccgagc
     2221 cacctcataa gcgtcgcgtc aattttcggt gaaggcgagt tgcgtcaccc ggtagcttcc
     2281 agcgtgtctg gcgggcgctt ggcctgccgc gaacgaagga gcggcgatgg gctttgacca
     2341 gtaccacgag cctcccgaag agcttcccgc aagggcgcgc accttcgccc gcctctgcgc
     2401 ctcgctcacc gaagaggccg aggcgatcgg ctggtatgag cagcgacttg ccgtcgagcc
     2461 ggaacccgag gcgcgcgcga tcatgagcga cgctcagggc gaggagttca agcacttcag
     2521 catggacctc gaggccctcc tcaggcggac gccggcgtgg cgggagatcg cggagggcat
     2581 cctgttccag cccggcgaca tcgtggagca cggggaggag gccgaggcgg agacgctcga
     2641 ggccggaccc gagacctcca caccgggtga gccgtcgggc tcgctcggca tcggcagcct
     2701 acgcggggtc gcgtgatgaa ccacctcctt cgctcgcacg cgccgatctc ggatgccgga
     2761 tgggagctgc tcgacaacga ggcgaaggag cggctcagcg cggcgctggc ggcgcggcgc
     2821 cttgtcgatt tctccggccc gtacggctgg gagcgttcga gcagcaacat cgggcgctca
     2881 cagccactcc cggacgcgct ctgcgagggc gtctctgcgc ggcagcgccg ggtgctgccg
     2941 ctgatcgagc tccgcagcga gttcacggtc tcccgtgagg agctacgcga cgccgatcgt
     3001 ggggctccgg acgccgacct aggaagcctc gaccgggcaa cgcgccagat cgcgctcgcg
     3061 gagaacatcg ctgtgttcca cggcttccag gcggcgggca tggccggcgt cgccgacgcg
     3121 agcccgcacg cgccgatcac gttaagcgac gacttcgacg actatcccgg acacgtggcg
     3181 aaagccgtgg agctgctgct cgaagtcggc gtggatggcc cgtacggcct cgcactcggg
     3241 ccggacggct acaccggcgt gatcgagtcg accgagcacg gcggctacct cgtgttcgac
     3301 cacctgcgca agatcctcga cggccccgtt gtgtacgcgc cgggcgtgcg gggcgctgtc
     3361 gtgctcagca cgcgcggcgg cgacttcctg ttcgagtcgg ggcaggacct gtcgatcggc
     3421 tacgaccacc acgacgccgg gtcggtgcac ctctatctcg aggagagctt cagcttcttc
     3481 gtcgcgacgc ccgacgccgc ggtgtcactc actgccgcgt agccgccaga gcgcctgacg
     3541 ctcgccgaag gtgagcaggc gccagtcgtc ttgggcctcg tcccacaccc agacgctcac
     3601 gtcgacgacg ctccgcgcgc cctcgagcgc gcggatcgtc tcgcgtaggt cggtgccgtc
     3661 cgcgatcacc tgccgcgaca tcgcgtcgac cacgcggaac gtgcgcggct cggcctgttt
     3721 cgccggttcg ggctccctac gacggcgtgg ctcgacgacg accgggccac ttccgccggc
     3781 cccgccgttc agcttcttct cgatgggcac catctgggcg ccggtgccct tcgtctctgt
     3841 gtcgagaacc caggtttttc ccatttctat gctgaattct agaagccggg cgtgcagcgg
     3901 ccgttgcagc cgtaagcgtg aggcgattcc gcggcgaaca gacccccatg gcagcacgag
     3961 cggctctgtt cggagtgctg gccgcgctcg catggcccgc gggagcgttg gcagtgatcg
     4021 gcgacacaag cggagggacc gccccacccg cgggggcacc ggcgcccacg acggttgctg
     4081 ccgtgacgct cacggccggc gtcgcgcgca cgagcgcgac ggcgccgcgc gctgtccaac
     4141 ttgcggtgcg cgccggtaat cgcctcaagc acaaggccta ccgctacggc ggcgggcaca
     4201 agagcttcaa ggacgccgcc tacgactgct cggggacggt gagttacgtg ctgcacgcgg
     4261 cgaagctcct ggcatcgccg ctcgattcga gcggcctcat gacctggggc gctcctggcc
     4321 cagggtcgtg gatcacggtc tacgcgaacg cctcacacgc gttcatggtg gtgggcggtc
     4381 tgcgcctcga cacgagcggg ccgggaccgt ccgggccgcg ctggcgcgca ctgccgcgct
     4441 cgctcgacgg cttcaccgcg cgacacccgc tggggctcta acggaccgtg gatgccggcg
     4501 cctgctgtgg cgccggatgg tgcggcttgg acgagccgct cagcacgaag ctagggacca
     4561 tgaacgccac agccacgagg ccgatcgtga tagcgcgcag gcgccggggc gtcagccaaa
     4621 cggcgccgtg attctgcagc caggctcgag cgccggacgc tccggcggcg gcggccattg
     4681 cgcattgcac gcacatgact cagaggttac gcgcctcggc tccctggcgg cgctgcaccg
     4741 cccaggcgcc gaccgccagt gccaccacag cgagaaccgc ggcgtagtcg gcgtatttga
     4801 agtcgtggtg gaagctctcc cagctcccgc ccagggccca gccggcaccc gcgaacgcga
     4861 agcaccagat caacgacgcc gcgagcgtga gcccgacgta tcgggcgatg ggagtcccga
     4921 acacgccggc tgggatcgag atgaacgacc ggacaagagg agtgagccgg ccgaggaaca
     4981 ccgcgagcga ctcatgcgaa tcgaaccagc gctcggcgcg tccaaagcgg tcgggcggga
     5041 ggtggagcca gcggccgtga cgttccacga acggacggcc tccgcgattt ccaatcaccc
     5101 aaccgatcag ggatcccgcg agatatccga gcgagccggc gagcgcgagc actatgtagc
     5161 tctcgatgcc gctcgagagg tgagcaccga acagcacggc tgcctgcccg gcgatggcgc
     5221 cggacgcgag cacgccggcg tagagcatcg tcagctcacc gcccaggggc agcagcgcgt
     5281 cgaccgccat gatcgtgaac acggcgtaca cgccgtggtt cccgatccag tcggtgagcg
     5341 tgttggtcag ggacgcaagt gggaggccgg ccggcatcgt tgcggcagcc tcgcaacgca
     5401 agctgaggaa ttcctgaatg tcgctgttgt ctcgcggcgt ctgcggcgag acccgtatcg
     5461 atccgcagcg ctaccccgcg tgggctagcc agctctaggc gtgggtgact ttagggtcga
     5521 atagcgacct caatgtcacc cacgtcccga ccactaccgc tgctgggtta acccggccta
     5581 ggcgtgggtg accttaatcg ccataagagc gaggaaagtc acccaccctg gctgcgtgcg
     5641 cgggatcgcg ctgccgttcg acggtcggac accacccgtc cgggcgcgca gctgaccgtc
     5701 gaggagccgc gtgtgagagc ctccgtgctg tggctgacca caaggaagag ctgctccagc
     5761 tgatcgaggg ctctccgaac ccgccgctca tgctcggcgg aatggccgag acgatcgggg
     5821 tctcctacga ggaggccgag cgcctcctcg aggagctcgt ccgcgaaggg cgggtgcgga
     5881 tggagggcga aacgcccgtg gctgtcgagc cgccggatcg gtaggccgat ggcaactgcg
     5941 ctgttcgtgt gcctgcacaa cgcgggccgg tcgcagatga gccaggctct gttcgagcgg
     6001 gccgcggcag gccgccaccg cgcgctgtcc gccggcacga ccccgggcga ccgcgtgcat
     6061 ccggaagttg tggacgcgat gcacgagctc ggcatcgacc tgtccggcaa cgttccgcgc
     6121 aagctcacgc gcgagctcgc cgaggaggcc gacgtggtgg tgacgatggg atgcggcgac
     6181 gagtgcccgt acatcccggg caagcgctac atcgactggg atctcgagga cccgaagggg
     6241 cgcccgctgc acgaggtgcg ggagacccgg gacgagatcg agcggcgcgt ccgtgagctc
     6301 gtggccgagc tcgacggctc ggcctgagac ggagcggccg cgcgtgacga ccgccccgct
     6361 ctggcggtct cagctgcgga gcaccggagc gtcttcccgc tccggctcgg ccgtcggctc
     6421 gtccgccgac tccttctcca ctacgggcgt gaccttttca ggccgctcgc gagcacggtc
     6481 atagaggtcg cgcaactcgc gctcgaagct ctcgtcgcgc ttctcatacc agcgcctgct
     6541 gtgacacatg cgcaccacct ccgtggcggc tcgagaacca cactggatgt tgacagggtc
     6601 agcgcacgcc cgtaagacgg ctcatcgtcc gatcgagcat ccggtcgggc aggacagcgg
     6661 caagcgtggc ctgcacgcgc gcgtcgatgc ccaccaggta gcgcgtgcgt ggtcgatccg
     6721 cagtcagggc gtgcagcacg gcgcgggcga ccttctcggg cttcacgcca cgcctcactg
     6781 cgtccgttgc gaccttctcc gtggcccgga tgcgatcgcc gtagaggcgg cgaccctcct
     6841 cgggaagcga gtcgaacagc tcgttgcccg cgcggatgcc cttttcccag atctcggtct
     6901 tcacgctgcc cggctcgacc agcgaaacct cgattcccga gggggtgagc tcgcgccgca
     6961 gcgagtcggc gagcccttcg acgccgaact tcgcggcgtt gtacggcgag atgaacggcc
     7021 cggccatgcg gccgccgatc gagccgatga tcacgagtcg tccgcgcgag cggcgaagcg
     7081 ccgggatgaa ggccttcgtg agagcgaaag gcgcgaccag catcacgtcg agttggcgcc
     7141 gaacgtcttc gagggggagg tattcgagcg gtccgggtag cgccatgccc gcgttgttca
     7201 cgactccggc aagatcgccg tccactcgat cagggagcga cgcgatcgcg tcgaagtcgg
     7261 tgacgtcgag catcagcggc tcgatccgcg cgggatcgag cgcccgcacc gcttcagcgt
     7321 cctcttcgcg cctcacgccc gcgagcacgc ggaagccgcg cgcggcaagc tcggcagccg
     7381 tggcccgccc tatgccggtg gacgtgccgg tgatcagcac ggtgcgatca gccatcgcgg
     7441 cgcgacttta acggccgagc cgcaccgccc gctcgaagtg gtgtgagcgc ggggagcgcc
     7501 gcaactctcg ccagcgcggc tgttatacgt aagcagaccg cctaatccag ccgcgtcccg
     7561 cgcggctgga gcgggggacc cactggccga tgaaccggct gggggcgaat cggccgcaga
     7621 gcggccgacg cgctgtctcg agcgcgagcc cgtcagctaa ccccgcaggc agccagagac
     7681 aacgtgcacc aattcccgca acggctcctc ccgccggcca tgctcgcgct ggccgcgctt
     7741 tcgctaacgg ccgccgcgcc ggcgtcggcc aagccgcgta cgcccgcgcc gaagatcagc
     7801 tcggtgcgct gctggccggt gagcgcatgc acgagcgatc cacacacggt gcgcgccggc
     7861 ggccgcctcc gcttccgcgg acgcaacctc aagcacggca tgcaggtgcg cttccagggc
     7921 acggtgatcg cggggaagaa gaccgcgctc gtcgccaaac tgaggctgcg gcgaccggac
     7981 ggcttcgtgg cgaccgtgcc caccaccgcg cgctcgggcc gcgtgcgcgt tgtgtctccg
     8041 accggtaggc gctcgaacct cgccggcccg atcacgatcg tgagcacacc ggtgaagggc
     8101 accggcaccg cgctcgacgg caacggcgtg tggatctggt a
//
LOCUS       DASVXS010000288         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1054984, whole genome shotgun sequence.
ACCESSION   DASVXS010000288 DASVXS010000000
VERSION     DASVXS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054984"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VF752_16170"
     CDS             <1..637
                     /locus_tag="VF752_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857131.1"
                     /translation="RRRPSLPARRTLAIALAAVVALAGAAFAASPPLRHSVLDWLGLR
                     GVKVEQVPKLPKPAGAGERLGLGRRTSLAAARQAVPFRLTVPTALGAPNEVWLSRTIP
                     RGQVSFVYARGGHVRALVTQFVAQQPFTFLKKFATPQTRVERVRVGGSPGAWIAGLPH
                     EVAYVDADGTVRAETLRLATNTLIWQRGGVTLRLEAHVSKARALEIARSFR"
     gene            699..1208
                     /locus_tag="VF752_16175"
     CDS             699..1208
                     /locus_tag="VF752_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857132.1"
                     /translation="MRKALPLIAVAVAALALAAPALAKEGVSLSSTPDGLAPGQDWNV
                     RITPIFHDGGGPPSGPKGIAIQRGASGHWLRFPAHRVSGGDYEATVSFPREGRWTYKV
                     TGFGLANQYFDPVVIERSVPVPAASHSDGGGGDGFPFAAVFGGLGGLLVLGLGAVAIR
                     RVLVRPASS"
     gene            complement(1171..1956)
                     /locus_tag="VF752_16180"
     CDS             complement(1171..1956)
                     /locus_tag="VF752_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857133.1"
                     /translation="MFFGWDNTVLIASQAVCVALPAAGLPRWLERYRSNKWVLILPLS
                     IAVVIAAISAFPGTADILTWVAFLLVPPGCALALGWAMHGARRWLALLAIPALAIAWA
                     SQDTRAGQIAALVLVAGSCVMVGRLLSGAAPLPLLKVGIVAMAVVDSILVFGNQLQAP
                     NAVLEAARPGFGLPHLQSVAFGGATLGYGDLFIAGVLGGVLAAERGPQLAAALGVFVV
                     ALAWDQLFLVYDLLPATVPVAIVLVVIEGVRVTRRRAAPARAG"
     gene            complement(1967..2851)
                     /gene="era"
                     /locus_tag="VF752_16185"
     CDS             complement(1967..2851)
                     /gene="era"
                     /locus_tag="VF752_16185"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX8857134.1"
                     /translation="MTHSGFVALAGRPNVGKSTLVNHLVGEKVAIVSDKPQTTRRAIR
                     GVATGDDWQLVLVDLPGVQRPRDPLTARMQARMQYELSESDAALFVLSGEQGVGPGDR
                     FIAAAIREVGLPTVTAVNKADRLRRRGMVEALSAAGELDVQGEIFPISARTGEGVEDL
                     QSALIELMPQGPFLYPPEERTDLPENVRLAELVREQVLARTRQELPHAAEVEIDELIE
                     RDEDGPLTVVARIWAETDSQKRILVGAGGRMVRDIGTAARIEIEALTGRRVHLDLNVR
                     VRRGWRQDEALLDRLGID"
     gene            complement(2848..>3287)
                     /gene="ybeY"
                     /locus_tag="VF752_16190"
     CDS             complement(2848..>3287)
                     /gene="ybeY"
                     /locus_tag="VF752_16190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX8857135.1"
                     /translation="TATSSARRPSCARRSAIGPIADTLEIEVSGPAPDGAGHAAKAAF
                     EAAGVRDGHVAIAFVGEHEIRELNREHRGKDTPTDVLSFPVDGVGPTQGPRELGDIVI
                     CPEHTEDVIEAIVHGALHLCGYDHETDDGEMLALQDRVMEGLR"
BASE COUNT          472 a         1189 c         1157 g          469 t
ORIGIN      
        1 ccgccggcgc ccctcgctcc ccgcgcggcg cacactcgcg atcgccctgg ccgcggtcgt
       61 ggcgcttgcg ggcgcggcgt tcgccgcttc gccgccgctt cgccacagcg tgctcgactg
      121 gctcggactc cgcggcgtga aggtggagca ggtgcccaag ctgccgaagc ccgcgggcgc
      181 cggcgagcgg ctcgggctcg gccgccgcac gtcgctggcc gccgcgcggc aggcggttcc
      241 gttccgcttg accgtgccca ccgcgctcgg cgcgccgaac gaggtgtggc tgagccgcac
      301 gatcccgcgc ggccaggtga gcttcgtcta cgcgcgcggc ggccacgtgc gcgccctggt
      361 cacgcagttc gtggcgcagc agccgttcac cttcctgaag aagttcgcga cgccgcagac
      421 gcgggtcgag cgtgtgcgcg tcggcggctc gcctggggcg tggatcgcgg gcctgccgca
      481 cgaggtcgcg tacgtcgacg ccgacggcac ggtgcgagcg gagacgctcc gacttgccac
      541 gaacacgttg atctggcagc gcggcggagt gacgctacgg ctcgaggcgc atgtatcgaa
      601 ggcgcgcgcg ctcgagatcg cgcgctcctt ccgctgaacg cgcgcgggaa cccgcctcgc
      661 gcggcggttg tagagggggt agcaaccaag gaggccccat gaggaaggca ctgccgctca
      721 tagccgtcgc tgtagccgct ctcgcgctcg cggcgccagc ccttgcgaag gagggcgtct
      781 cgctcagctc cacgccggac ggacttgcgc ccggccagga ctggaacgtt cgcatcacgc
      841 cgatcttcca cgacggcggc ggcccgccgt ccgggccgaa gggcatcgcg atccagcggg
      901 gtgcgagcgg gcactggttg cgcttccccg cccaccgcgt cagcggcggc gactatgagg
      961 ccacggtgag cttcccgcgc gagggcaggt ggacgtacaa ggtcaccggc tttggacttg
     1021 ccaaccagta cttcgatccg gtcgtgatcg agcggtctgt tccggtgccg gcggcaagcc
     1081 actcggatgg aggcggtggc gacggcttcc cgtttgcggc cgtcttcggc gggctcggcg
     1141 gtctgctcgt gctggggctc ggagccgtag ctatccggcg cgtgctggtg cggcccgcct
     1201 cctcgtgacg cggacacctt cgatcactac gagcacgatg gctaccggca cggtcgccgg
     1261 caacaggtcg tagacgagga acagctggtc ccacgccagg gccaccacga acacgccgag
     1321 cgcggccgca agctgagggc cgcgctcggc ggccagcacg cctccgagca cgccggcgat
     1381 gaagaggtcg ccgtagccaa gcgtcgcccc accgaacgcc accgactgaa ggtggggcag
     1441 accaaagccg gggcgtgccg cctcgagcac ggcgttcggc gcctgcagct gattgccgaa
     1501 cacgaggatc gaatcgacga cggccatggc aacgatgccc accttcagga gcggaagcgg
     1561 agcggcgccc gacaggagcc gcccgaccat cacgcacgag cccgccacga gcaccagcgc
     1621 ggctatctgg ccggcgcgcg tgtcctgcga cgcccaggcg atggcaagcg cgggtatcgc
     1681 gagcagagcg agccagcgcc gggctccgtg catggcccag ccgagcgcga gcgcacatcc
     1741 cggcggcacg agtaggaagg cgacccacgt gaggatgtcg gcggtgcccg ggaaggccga
     1801 gatcgcggcg ataacgacgg ctatcgacag cggcaggatc aagacccact tgttcgagcg
     1861 gtagcgctcg agccaccgcg gcaggccggc cgcgggcagc gcgacgcaca cggcctgcga
     1921 tgcgatcagg acggtgttgt cccagccgaa gaacaccgcg tgcggatcag tcgatgccga
     1981 ggcggtcgag cagcgcctcg tcctgacgcc agccgcggcg cacgcgcacg ttcagatcga
     2041 ggtgaacgcg ccggccggtg agcgcttcga tctctatccg ggccgccgtg ccgatgtcgc
     2101 gcaccatgcg accgccggcg ccgacaagga tccgcttctg cgaatccgtc tcggcccaga
     2161 tgcgcgctac cacggtgagc ggcccgtctt cgtcgcgctc gatcagctcg tcgatctcga
     2221 cctcagcggc gtgcggcagc tcctgacgcg tgcgcgccag cacctgctcg cgcaccagct
     2281 cggcgaggcg cacgttctcg gggaggtcgg tgcgctcttc gggcgggtag aggaaggggc
     2341 cctgcggcat caactcgatg agtgcgctct gcaggtcctc gacgccctcc cccgtgcgcg
     2401 cgctgatcgg gaagatctcg ccctgcacgt cgagctcgcc ggcggcgctg agcgcctcca
     2461 ccatcccgcg ccggcgcagg cgatcagcct tgttcacggc cgtgaccgtg ggcagcccga
     2521 cctccctgat cgccgcagcg atgaagcggt cgcccgggcc cacaccctgt tcgccgctca
     2581 gcacgaacag cgccgcgtcc gactcggaga gctcgtactg catgcgcgcc tgcatccgcg
     2641 cggtgagcgg gtcgcgcggg cgctgcaccc ccggaagatc cacgagcacg agctgccagt
     2701 cgtcgccggt cgcgacccca cggatcgccc gccgcgtggt ctgcggcttg tcggacacga
     2761 tcgcgacctt ctcgcccacc aggtggttca ccagcgtgga cttgcccacg ttcggccggc
     2821 cggcgagcgc gacgaagccc gagtgggtca ccgcagacct tccatcactc gatcctggag
     2881 cgcgagcatc tcgccgtcgt cggtctcgtg gtcatagccg cagaggtgga gcgcgccgtg
     2941 gacgatcgcc tcgatcacgt cctctgtgtg ctccgggcag atcacgatgt cgccaagctc
     3001 acgcgggccc tgcgtcggtc ccacgccgtc gactggaaat gacagcacgt cggttggggt
     3061 gtccttgccg cggtgctccc ggttgagctc ccgaatctcg tgttcgccca cgaacgcgat
     3121 cgcgacatgg ccgtcgcgga ctcccgccgc ctcgaacgca gccttcgcgg cgtgtcccgc
     3181 cccgtccggc gcgggtccgc tcacctctat ctcgagcgtg tcagcgatcg gtccgattgc
     3241 gctgcgccga gcgcagctcg ggcgcctggc gctgctggta gcggtcg
//
LOCUS       DASVXS010000289        18492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_698630, whole genome shotgun sequence.
ACCESSION   DASVXS010000289 DASVXS010000000
VERSION     DASVXS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18492
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698630"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VF752_16195"
     CDS             complement(<1..1201)
                     /locus_tag="VF752_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8857136.1"
                     /translation="MKETLEERTRYQPEEVESRVFARWDEAGIFHPEPEGSPSENFSI
                     AIPPPNVTGALHMGHALNGTMQDLVVRMRRMQGRRTKWIFGTDHAGIATQVKVEQQLL
                     DEGTSRQEIGREAFIERVWEWREKYGSTIVEQYKRLGASADYEDERFTMDEEYQRAVT
                     HVFVKLFEKGLIRRDNYMVNWDPGTRSAISDLEVEQRRVTDTLYSIVYPLADGSGSVT
                     VATVRPETMLADTAVAVNPGDDRYRDLIGKEAVLPLVGRRLPIIAEDYVDTEFGTGAL
                     KITPGHDPNDFEIGRRHGLEEVTVIGEDGLMTDAAGEFAGMNVDEARDAVVAALEAEG
                     AIAGTEPYEHDVPHSHRSGMRIEPLISLQWFCDMTGLAKPAIDVVKSGQVKFWPEKPW
                     TDVYLGWL"
     gene            1363..1677
                     /locus_tag="VF752_16200"
     CDS             1363..1677
                     /locus_tag="VF752_16200"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8857137.1"
                     /translation="MTAASLIRSARRKAGLSQAELAARLGTTQSAVARLESERSNPRL
                     STLEQALAAAGRRLELKSAPAPASQDESLISRRLALTPGERVTAFESAYRNTKSLLAS
                     RR"
     gene            1683..2171
                     /locus_tag="VF752_16205"
     CDS             1683..2171
                     /locus_tag="VF752_16205"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX8857138.1"
                     /translation="MGLPEFDPRALLEALTRRGVDFVVVGGFAAVVHGSPRLTQDLDI
                     TYATDRVNLAALAEVLLELGARLRQVDEDLPFEPDERALARTEMLTLTTSAGALDLLA
                     KPAGAPAYARLRERAERVDLGGMAVLVASIEDLVAMKRAAGRAKDLADIEELEAIRRL
                     RA"
     gene            complement(2160..2825)
                     /locus_tag="VF752_16210"
     CDS             complement(2160..2825)
                     /locus_tag="VF752_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8857139.1"
                     /translation="MKQLADGLHHLSGFPPNIMNVYLADGVLIDAATRHARRRILRQL
                     QGREVTAHALTHVHPDHQGSSKVVCDTLGIPLWCPEGDADVMENGRIAARQPAHPMNK
                     LSERVFAGPPRKVDRRLREGDEVGGFTVIETPGHSAGHVAYWRPADRALILGDVLFGL
                     NPSTGVPGLHWPPDHFTPDPARNRESARKLADLEPALICFGHGPPSRDTRKFQEFVRA
                     SGA"
     gene            complement(3015..4631)
                     /locus_tag="VF752_16215"
     CDS             complement(3015..4631)
                     /locus_tag="VF752_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8857140.1"
                     /translation="MTRSRSVVVSALVCALALAVPALAHATTKDFTNSASITIPGSGT
                     GAGTGAPASAYPSTVDVSGMGSQIDHVSVILHGLSHTFENDIDMMLVSPSGQALLLMS
                     DVGDGSDFSNVNLTIDDSAGTTFPTTGVIGSGFFKPTNVGGGDPFPAPAPAPSLGSLG
                     AFTGNPNGTWSLYIVDDATVDSGTLAGGWTLRLTTSPVAPTVTLGSDLKTGGSLLCGA
                     PGGCSFMQSTIPGRVVATPIDGVITRFYTRDFGPGSQIEFRVIRPAAGGNFQGAGSTE
                     TFTTPSGFDPLGTYATRLPVKGGDLIGYDLDGSDGAGVVTTGGATSSYFTPKLADGAF
                     APPSASPGWEYLVGADLEPDADADGYGDVTQDGCPSNPAVHDACPAAPQQPQQPGTQL
                     NKTKACANVKAGTGKKDLLTGTPFGDLLNGLAGDDTLNGGDGNDCLNGGAGNDKLNGG
                     KGNDKLSGGTGNDRLNGGDGTDSLSGGAGNDNLSGGKGKNTLSGGSGNDKLNIADGKK
                     DSVNGGKGRDVCTGDPKDKFKSCETKKIKR"
     gene            complement(4767..6041)
                     /gene="clpX"
                     /locus_tag="VF752_16220"
     CDS             complement(4767..6041)
                     /gene="clpX"
                     /locus_tag="VF752_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX8857141.1"
                     /translation="MARPTDSNEQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEELTGPATFSLENLPKPKEIHDVLQDYVVGQESAKRTLSVAVYNHYKRVQMMQSD
                     ENDLELQKSNILLLGPTGCGKTLLAQTLAKILNVPFAIADATALTEAGYVGEDVENIL
                     LKLIQAADYDVKKAETGIIYIDEVDKIARKADNPSITRDVSGEGVQQALLKILEGTVA
                     SVPPQGGRKHPHQEFLTIDTTNILFICGGAFANLDKVIERRIGHKGVGFGASVRSKTN
                     KDTGELFEQILPEDLVEYGLIPEFIGRLPVISVIHQLSREDLVLILNEPRNALVKQFQ
                     RFFQFDGIELVFADDSLESIADKALDRETGARGLRSILEETLLDVQFELPSRRDVKKC
                     VVTKETIEKGLKPTLVTEAVADERAADQAESA"
     gene            complement(6075..6692)
                     /gene="clpP"
                     /locus_tag="VF752_16225"
     CDS             complement(6075..6692)
                     /gene="clpP"
                     /locus_tag="VF752_16225"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX8857142.1"
                     /translation="MERNERKRSEHMSPLVPMVVEQTSRGERAFDIYSRLLNERIVFL
                     GTPVTDEIANLIVAQLLHLESEDPDKDISLYINSPGGSVYAGLAIYDTMQFIKPDVQT
                     ICVGIAMSMGALLLAGGADTKRMALPNAKILIHQVSSSFQGQATDIEIHAKEIIDVRR
                     RLDEILAKHTGQELEKVARDTERDYFMSAEEAKDYRLIDRVISQH"
     gene            complement(6773..8185)
                     /gene="tig"
                     /locus_tag="VF752_16230"
     CDS             complement(6773..8185)
                     /gene="tig"
                     /locus_tag="VF752_16230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX8857143.1"
                     /translation="MPAALSTKTTELGDSRVRVEVEVPSAEVERELDSAARALGQEMR
                     ISGFRRGKVPPPVVIQRVGREAVLEEAIRRGLPNWYEEALADTRLATVGDPKLDVGDY
                     PEKGSPLSFSIEVGVRPDAKLGSYKGLEVGRREPEVANEDVDRELERLRESQASLETV
                     DREAQNGDFVVIDFVGTLDGDPFEGGEARGYTLELGSERLIPGFEEQLVGAAAGEQRE
                     VRVTFPDDYGAEHLAGNEAVFDTTVKEVKEKRLPELDDDFAAEAGGFDTLDELRSDIE
                     AKLRQADERTIENEFREAALDAAVAEAKVDVPHELVHAKAHEMWHETAHQLQHRGMDP
                     RMYLQMMGKEEEELVTESEPQAEQALKRESVLAAIVAAEGIEVSDDELLDALRDAAIR
                     QGGQPPSEKSLRRSLDRAKQQGRAEALREDIAMRRALDILVESAKPISVEQAKARDKL
                     WTPGKEEGEAAPQQIWTPGS"
     gene            8184..8369
                     /locus_tag="VF752_16235"
     CDS             8184..8369
                     /locus_tag="VF752_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857144.1"
                     /translation="MASHDSVSDLTRALTDRGRRERLVEWLYTGPLGHLYGVLADLVQ
                     LWVRYGVRRLRLRVSRR"
     gene            complement(8366..8818)
                     /locus_tag="VF752_16240"
     CDS             complement(8366..8818)
                     /locus_tag="VF752_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857145.1"
                     /translation="MEFSAGPVTPSGAAPASDATLQRDRNVALASTFLLLIGMPVGWL
                     PGSTGDAVGMAGAIIIVLAIMAGLFLWLLPRERAAGRASRTALILGVLAIAVGFVFWT
                     GLPFAVGAAALALGLGARESGTGDGRATAGAILGGIAVVASFVLLLIG"
     gene            8896..10224
                     /locus_tag="VF752_16245"
     CDS             8896..10224
                     /locus_tag="VF752_16245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8857146.1"
                     /translation="MPERYDAIVIGAGPSGEVAAGDLANHGLRVAAVERELVGGECGF
                     WACIPSKTLLRPGDVLSEAGQAPGAREAVDGSLDVRAALSWRDFEVSNYDDHHQADWL
                     SEMKVDILRGQARLDGPGRVRVGDELYETDRVVVATGSDPVIPPVDGLAELEGLWTNR
                     EATGLKEVPESMLTLGGGPVGVEMGQVLRRMGARVTIVEHEGRLLSREARPVGEAVAE
                     ALASQGVELRLGRAATAARHADGGFELDLDDGSTERAEKLLVATGRKPRIDDLGLESV
                     GIEPGKRGIEVDERMRAGEGVWAIGDATGKFLFTHVGKYQARIAVADILGKPAHADYR
                     AVPRVVFTDPEVAAVGATEGERTARAELANIARTSTYTREYDQNPGFLELVADGDRLT
                     GAYAVGPQAGEWMQQITLAIRAEMPLDVLRDVIQPFPTFSEVVYAALKQL"
     gene            complement(10228..10959)
                     /locus_tag="VF752_16250"
     CDS             complement(10228..10959)
                     /locus_tag="VF752_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857147.1"
                     /translation="MADRDQAKSGGVSLDTIIVASLASVTAMLITSHFWKGGTVISAA
                     MTPVIVALASEAYRRPARKVTQLAPAVKRLAPVAESRQPARGRARGGTLTASRPQQRT
                     FERNGGSSSGNGGGVTIYRPRRNLHVKAAIATGLVAFVIGALVLTVPELVFGGSVTTG
                     SRTTLFGGGHSSASRDRQKTTTQPEQTQPQQNPQSSPTPTTPAEPPAQTAPAQPPAQT
                     APAPQATPPPSNGGSAPPAQSAPTP"
     gene            complement(11154..13334)
                     /locus_tag="VF752_16255"
     CDS             complement(11154..13334)
                     /locus_tag="VF752_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857148.1"
                     /translation="MIKRLIVASVAATALGLAFIAPSAGASVSVGHSGWSWGNPLPQG
                     NTLRALDFAGGRGFAAGDFGTLLRTDDAGATWSGVPTGITADLKRVSVVDSNTIVIAG
                     GCTARRSDDGGQTFHRLPFSNSDINCPAQISSFSFPTPQAGFLLLQDGTVLSSADGGQ
                     TFSRQTAVPGTPATGAGTPATPADIFFTSPTVGYVITRGAGGGKIYRTTNGGAAWTLV
                     TTAGANLNALWFENAGTGYAVGDGKTLVATTDGGDNWAAKPLGGVSGGNFGSIRCVGS
                     TCILATVDGATLVRTTDGGGTGSEVSPSSQRVYVAGFASPTRVVAVGEAGSTVASDDG
                     GQNFSPIGARLAGSFFELVATSGTVAWAPGANGSLARTTNGGQSWSGGGVPTSEDVVD
                     VSFPDGSNGYALDSSGSLFKTTNGGGSWALLDTGTSATPPSVLALDANRVLLVGPQGV
                     RRSTDGGQAFSRINAKPVRNANLDSVDHVGSTVFVYGSKAAYFSTNGGAKWQQLKLPQ
                     KRFRVNDLDFTTSKAGYVLESNGRLWFTKNRGAKWTQLVGTGTLDVVGIDFSSTKTGF
                     LVIRRFGFQTGGLLLRTSDAGHTWRPQLVSRNSINDLSLVSSGGTDYVRDNANNMFFT
                     RTGGDLGTASSLRLKTKKTKLTKASTIKVTGKLPGAQGGEQVVVSARSVKGGWVHKVV
                     QVASNDSFSTVWKVRRDTIFVAQWLGDADHNGAGSKVLGVSFKK"
     gene            complement(13449..14129)
                     /locus_tag="VF752_16260"
     CDS             complement(13449..14129)
                     /locus_tag="VF752_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857149.1"
                     /translation="MRGSGLKIVLSAGLVTVALVCAVQPAGAAVRSKTFRDDASGLRG
                     TYKVTVTRVSAVALRVRTDASFRASRSGHLTLFSGCQEIYCTIESWPFDYKKGSNRLV
                     FENVASGFGDQFDNSGGGGGTSGGGQSLIDQLLSGLPVLGPIVDPVLGPILDPLPAVG
                     ASRSTLTRHQRRVLAARKRMARRRARAHLTQIREAAARLLPVRCGQSSLYVPGAGQVA
                     DSTGICVP"
     gene            complement(14203..14526)
                     /locus_tag="VF752_16265"
     CDS             complement(14203..14526)
                     /locus_tag="VF752_16265"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8857150.1"
                     /translation="MRQAAADEVFTALADPTRRALLEALSRGAPETSSRLAAELPITR
                     QAVEKHLAVLGRAGLVERTREGREARYEMTPAPLADAASWIAAVGGEWDDTLARLQRA
                     VERRR"
     gene            complement(14533..14853)
                     /locus_tag="VF752_16270"
     CDS             complement(14533..14853)
                     /locus_tag="VF752_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857151.1"
                     /translation="MKVEREITIEATPGEVWEALSEPALLRDWLFEDGDERDLEVDEA
                     EEEQRLAFRWHREGQGETSVVFTLEAVPTGTRVLVVERGLRVPVTWGPALNALASVSS
                     MALA"
     gene            14969..15598
                     /gene="clpP"
                     /locus_tag="VF752_16275"
     CDS             14969..15598
                     /gene="clpP"
                     /locus_tag="VF752_16275"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX8857152.1"
                     /translation="MPLIPMVVEQSARGERSFDIYSRLLGERIVFLGQQVDDEIANLV
                     VAQLLHLESEDPDKDISLYVNSPGGSVYAGLAIYDTMQFIRPDVQTICFGVGMSMGAL
                     ILAGGAPGKRMALRNSRILIHQPSGGFQGTSADIEIHAREVIELRRRLDEIYAHHTGQ
                     PLEQVHDDMDRDRFFTAEQAAEYGLVDRVIESHELRRSAYGFRPNGAEG"
     gene            15662..16510
                     /locus_tag="VF752_16280"
     CDS             15662..16510
                     /locus_tag="VF752_16280"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX8857153.1"
                     /translation="MATHSRTPGSEHLARIERLRRLPPVAFAEHCGRKAFEMGAIDRA
                     IVLASQAYVAIFPFLIIVGSIAPRRGAKDTADGLIDRFGLTGSTAQSVRDLFSSPTDV
                     KNALSWAGVIVLLLSTLSFTRSLQRVYELCFSLPAAGARNTKRGLLWLGGLAVYGWVG
                     TLIALVRAGPLRNALAVVLSLAWSFVFFTWTPYVLTGYRVPWRRLVPIGFATTTGLAF
                     FVLASGVYMPHLISRQSHRYGLIGVAFALLSWVLVGAFVIVAAAVIGSEVSETPRRMY
                     RRLRGR"
     gene            complement(16464..17441)
                     /locus_tag="VF752_16285"
     CDS             complement(16464..17441)
                     /locus_tag="VF752_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857154.1"
                     /translation="MGIRAVEDGADLGHTLDAIDRPSAHQAVRGRVPLFERLYTSPRV
                     HRLLPDWLALGLAALRARLLWRFSGERRSRAIAQVSLMLEGTGREREAERIARRQIVE
                     QALRRELKLRPWVARRYPVVGIEHLEAAQAGGRGVLLMGLHFGPPRGTSAPLSVKGYR
                     LYMAGADWLYADVYEGRDGLLALLERRVFEDNGGRWVPRGGSFEVFRALLERGEVCLF
                     HFDVPGGTETTLLGKRARVGSGIARLARETGGLIVPSVSEGGCRHPRLRLHEPVDPRR
                     FASEQELVDHLAALATGFILDRTPESWEPNDFPPLIFRAAGDTYAWAFR"
     gene            complement(17487..18374)
                     /locus_tag="VF752_16290"
     CDS             complement(17487..18374)
                     /locus_tag="VF752_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEX8857155.1"
                     /translation="MSHDQLVQVRRFNRVVTRRAGALEDHFLARDRPLGEARVLWEIG
                     PDGSDVRTLRSRLGLDSGYLSRLLRSLEQAGLIVVERCDGDRRVYTAQLTAAGVKERN
                     VLDRRSDDLAESFLAPLSASQRERLVNAMGEVERLLTLGMVEIAPRDPTDPQAQFCLR
                     EYVSELDRRFDDGFDPKRSISADARELRPPAGVLLVASLGSEPVGCGALKFHPGEPTE
                     IKRMWVAESARGFGLGRRLLGELETLAVESGSRVVRLETNRTLTQAIALYRSAGYAEV
                     APFNDEPYAHHWFEKRIKT"
BASE COUNT         2714 a         6447 c         6297 g         3034 t
ORIGIN      
        1 cgagccagcc gaggtagacg tcggtccacg gcttctccgg ccagaacttg acctggcccg
       61 acttgacgac gtcgatggcc ggcttcgcga gcccggtcat gtcgcagaac cactgcagcg
      121 agatcagcgg ctcgatccgc attccgctgc ggtgcgagtg cggcacgtcg tgctcgtacg
      181 gctcggtgcc ggcgatcgcg ccctcggcct cgagcgcggc cacgacggcg tcgcgcgcct
      241 cgtccacgtt catgccggcg aactcgccgg cggcgtcggt catgaggccg tcctcgccga
      301 tcaccgtcac ctcttcaagg ccgtggcggc gcccgatctc gaagtcgttc gggtcgtgcc
      361 ccggcgtgat cttcagcgcg ccggtgccga actcggtgtc cacgtagtcc tcggcgatga
      421 tcggcaggcg gcgtcccacg agcggcagga ccgcttcctt gccgatcagg tcgcggtagc
      481 ggtcgtcacc ggggttcacc gccaccgcgg tgtccgcgag catcgtctcg ggccgtaccg
      541 tcgccaccgt gaccgaaccc gagccgtcgg cgagcgggta cacgatcgag tagagcgtgt
      601 cggtcacccg ccgctgctcc acctcgaggt cggagatcgc cgagcgcgtc cccggatccc
      661 agttcaccat gtagttgtcg cggcggatga ggcccttctc gaagagcttc acgaacacgt
      721 gcgtgacggc gcgctggtac tcctcgtcca tcgtgaagcg ctcgtcctcg tagtcggccg
      781 aggcgccgag ccgcttgtac tgctcgacga tcgtggagcc gtacttctca cgccactccc
      841 acacgcgctc gatgaacgcc tcgcggccga tctcctgccg gctcgtgccc tcgtcgagca
      901 gctgctgctc taccttgacc tgagtcgcga tgccggcgtg gtcggtgccg aagatccact
      961 tggtgcgccg cccctgcatg cggcgcattc gcacgacgag atcctgcatc gtcccgttga
     1021 gcgcgtgacc catgtggagc gcgccggtca cgttcggcgg cgggatggca atcgagaagt
     1081 tctcggacgg cgagccttcg ggctccgggt ggaagatgcc cgcctcgtcc cagcgcgcga
     1141 aaactcggga ctccacctct tcgggctggt agcgggtgcg ctcctcgagg gtctccttca
     1201 ccctccggag tctatttatg gagcccctgc aaaccttcgc ctggctgcgt catcgccccc
     1261 tcgcgtgcag agcacgccgc gggggctgct tccttgccat gctcggtttt cgcggctcat
     1321 ctcgtatgtg ctatagctcg ttagtgctat atcatttgcc gcgtgaccgc tgcttcgctc
     1381 attcgctctg cccgcaggaa ggccgggctg tctcaggctg agctcgccgc ccggctcggg
     1441 accactcagt ccgccgtggc gcgcctcgag tccgagcgga gtaatccacg tctgtccacg
     1501 ctcgagcagg cgctcgcagc ggccggccgc cggctcgagc tgaagtccgc tcccgccccg
     1561 gcgagccagg acgagtcgct catcagccgc cggctcgcgc tcacgcccgg ggagcgtgtg
     1621 acggcgttcg agtcggccta ccgcaacacg aagtcgctgc ttgcaagcag gcgatgacga
     1681 gcatggggct acccgaattc gacccgcgtg cgctgctcga ggcgctcacg cggcgcggcg
     1741 tggacttcgt ggtcgtcggt ggctttgcag cggtcgtgca cggctcgcct cgcctcacgc
     1801 aggacctcga catcacctac gcgaccgacc gcgtgaacct cgccgcgctc gctgaggtgc
     1861 ttttggagct cggcgctcgc ctacgacagg tcgacgagga cctgccgttc gaaccggacg
     1921 agcgcgcact cgcgcgcacc gagatgctca ccctcaccac atccgcgggt gccctcgacc
     1981 ttctcgcgaa gcccgccggc gcccccgcct acgcgcggct gcgcgagcgc gccgagcgtg
     2041 tcgacctcgg cggcatggcc gtgctggtgg catcgatcga agacctcgtc gcgatgaaac
     2101 gcgccgccgg tcgcgcgaaa gacctggccg acatcgagga gctcgaggcg atccgccgcc
     2161 tacgcgcctg acgcgcgcac gaactcctgg aacttccgcg tatcgcgcga tggcggaccg
     2221 tgcccgaagc agatcagcgc cggctcgaga tcggcgagct tgcgcgccga ctcgcggttg
     2281 cgcgcagggt ccggcgtgaa gtggtccggc ggccagtgga gtcccgggac gcccgtactc
     2341 gggttaagcc cgaacagcac gtcgccgagg atcagggccc ggtcggcagg acgccaatac
     2401 gcgacgtgcc cagccgagtg accgggcgtt tcgatcacgg tgaaaccacc cacctcgtcg
     2461 ccctcgcgca agcggcggtc taccttccgc ggcggccccg cgaacacgcg ctcgctgagc
     2521 ttgttcatgg ggtgggccgg ctgccgcgcc gcgatccggc cgttctccat cacatcggcg
     2581 tcgccctcgg gacaccacag cgggatgccg agcgtgtcgc agacaacctt gctcgatccc
     2641 tggtggtcgg ggtgcacgtg ggtgagcgcg tgcgccgtca cctcgcgccc ctggagctgg
     2701 cgcaggatcc gacgtctggc gtgccgtgtg gccgcgtcga tcagcacgcc gtcggcgagg
     2761 tacacgttca tgatgttggg cgggaagccc gagaggtggt ggagcccgtc agccagctgc
     2821 ttcacggccg cgcaccctaa cgaacggccc gccccctccg ccaggccccc gcacgccggg
     2881 cgacgaggcg ggcgccgcac gacgctgcga gacctggcgg acgaggcggg cctggtccgg
     2941 tctcgcagcg aggatgtacg gcgtccgtaa acgcccgatg aggcgggcgc gaagcgtcgg
     3001 ttaagggcga gcgctcagcg cttgatcttc ttggtctcgc acgacttgaa cttgtccttc
     3061 gggtcgccgg tgcagacgtc cctgcccttg ccaccgttga cggagtcctt cttaccgtcg
     3121 gcgatgttga gcttgtcgtt gccggaaccg cccgagagcg tgttcttgcc cttgccgccg
     3181 ctcaggttgt cgttgccggc accacccgag agagagtcgg tgccgtcgcc gccattgagc
     3241 ctgtcgttgc ccgttccgcc gctgagcttg tcattgccct tgccgccgtt cagcttgtcg
     3301 ttcccggcgc cgccgttgag gcagtcgttg ccgtcgccgc cgttcaacgt gtcgtcgccg
     3361 gcaagaccgt tcagcaggtc gccgaaaggc gtacctgtca gcaggtcctt cttccccgtg
     3421 ccggccttga cgttcgcgca cgctttggtc ttgttcagct gcgtgcccgg ctgttgcggc
     3481 tgctgcggag ccgcaggaca tgcgtcatgc accgccgggt tgctcgggca gccgtcctgc
     3541 gtcacgtctc catacccatc ggcatccgcg tcaggctcga ggtccgcacc gaccaggtac
     3601 tcccatccgg ggctggccga gggcggtgca aaggcgccat cagcgagctt gggcgtgaaa
     3661 taggacgatg tggcgccccc ggtggtcacc acgccagcgc cgtcgctccc gtcgaggtcg
     3721 tagccgatca ggtctccacc cttgacgggc agccgcgtag cgtatgtacc aagaggatcg
     3781 aacccgctcg gcgtcgtgaa cgtctcggtg ctgccggcgc cctggaagtt gccgcccgcg
     3841 gccggccgga tcacccggaa ctcaatctgc gagccgggcc cgaagtcgcg cgtgtagaag
     3901 cgcgtgatca ctccgtcgat cggcgtcgcg accacgcgcc ccgggatcgt gctctgcatg
     3961 aagctgcagc cgcccggcgc tccgcacaac agcgaaccgc cggtcttcag gtcgctgccg
     4021 agcgtgaccg tcggcgccac gggagaagtg gtcagacgta gggtccagcc gcctgcgagc
     4081 gttccggagt cgacggtggc gtcatcgact atgtagaggc tccaggtgcc gttcgggttc
     4141 cctgtgaagg cgccgagcga cccgagcgac ggcgccggtg caggtgccgg gaaggggtcg
     4201 ccgccgccga cgttcgtcgg cttgaagaag ccgctgccga tcacgccggt ggtcgggaag
     4261 gtggtccccg ccgagtcgtc gatcgtgagg ttgacgttcg agaagtcgct gccgtcgcca
     4321 acgtcagaca tgagcagcag cgcctgcccg gacggactca ccagcatcat gtcgatgtcg
     4381 ttctcgaagg tgtgtgaaag gccgtggagg atcacgctca cgtgatcgat ctgcgagccc
     4441 atcccggaga cgtcgacggt cgagggatag gccgacgccg gcgcccccgt ccccgctcct
     4501 gtgcccgaac cggggatcgt gatcgaagcg gagttcgtga agtccttcgt ggtggcgtgc
     4561 gccagcgcag gaaccgcgag cgccaacgcg cacacgagtg ctgacacgac gacgctgcgg
     4621 ctgcgagtca tgcgagtgcc cctccatggg acggtgacga gtccaaccgc gccagggtaa
     4681 gcgtcaaaag tgactttgga gtacgcgcct ggacgaacgt tctagcccct aatcaggggc
     4741 tcgtccgcgc gcttcgtcgg cggcggctag gccgattcgg cctggtctgc cgcccgctcg
     4801 tcggccacgg cctcggtgac gagcgtgggc ttgaggccct tctcgatcgt ctccttggtc
     4861 acgacgcact tcttcacatc gcggcgcgag ggaagctcga actgcacgtc gagcagcgtc
     4921 tcctcgagga tcgagcggag gccgcgcgcg ccggtctcgc ggtcgagcgc cttgtcggcg
     4981 atcgactcga gcgagtcgtc ggcgaacacc agctcgatcc cgtcgaactg gaagaagcgc
     5041 tggaactgct tcacgagcgc gttccgcggc tcgttgagaa tgagcacgag atcctcgcgc
     5101 gagagctggt ggatcaccga gatgaccggc aggcggccga tgaactcggg gatcagcccg
     5161 tactccacga ggtcctccgg caggatctgc tcgaacagct cgccggtgtc cttgttcgtc
     5221 ttagagcgca ccgacgcgcc gaagcccacg cccttgtggc cgatgcgccg ctcgatcacc
     5281 ttgtcgaggt tcgcgaacgc cccgccgcag atgaacagga tgttcgtggt gtcgatcgtg
     5341 aggaactcct ggtgcgggtg cttgcgcccg ccctgcggcg gcaccgaggc gacggtgcct
     5401 tcgaggatct tcaggagcgc ctgctgcacg ccctcgccgg acacgtctcg cgtgatcgac
     5461 gggttgtcgg ccttgcgcgc gatcttgtcg acctcgtcga tgtagatgat cccggtctcc
     5521 gccttcttca cgtcgtagtc ggcggcctgg atcagcttga ggaggatgtt ctccacgtcc
     5581 tcgcccacgt agccggcctc ggtgagcgcc gtggcgtcgg cgatcgcgaa cggcacgttc
     5641 aggatcttcg cgagcgtctg cgcgaggagg gtcttgccgc agccggtcgg acctagcagc
     5701 aggatgttcg acttctgcag ctcgaggtcg ttctcgtcgg actgcatcat ctgcacgcgc
     5761 ttgtagtggt tgtagaccgc taccgagagc gtgcgcttcg cgctctcctg cccaacgacg
     5821 tagtcctgga gtacgtcgtg gatctccttc ggcttcggca ggttctcgag gctgaaggtc
     5881 gcgggacccg tgagctcctc gtcgatgatc tcgttgcaga gatcgatgca ctcgtcgcag
     5941 atgtagacgc ccggacctgc gatgagcttc ttcacctgcc gctgcgactt gccgcagaag
     6001 ctacagagga gctgttcgtt cgagtcggtg ggccgggcca atgcctctcc tcccccggga
     6061 ggcgggagat cccgctagtg ctgcgagatc accctgtcga tgagccgata gtccttggcc
     6121 tcttcagcac tcataaagta gtcgcgctcg gtatccctgg ccaccttctc gagctcctgc
     6181 cccgtgtgct tcgcgaggat ctcgtccagg cggcggcgca cgtcgatgat ctccttggcg
     6241 tggatctcga tgtcggtggc ctgtccctgg aagctcgagg agacctggtg gatcaggatc
     6301 ttcgcgttcg gcagcgccat gcgcttggtg tcggcgccgc cggcgagcag aagcgccccc
     6361 atcgacatag cgatgcccac gcagatcgtc tgcacgtccg gcttgatgaa ctgcatcgtg
     6421 tcgtagatcg cgaggcccgc atacaccgag ccgccgggcg agttgatgta cagcgagatg
     6481 tccttgtccg gatcctcgga ctcgaggtga aggagctggg cgacgatcag gttcgcgatc
     6541 tcgtcggtga cgggagtccc caggaagacg atccgctcgt tgagcagacg tgagtagatg
     6601 tcgaacgcgc gctcacctcg cgacgtctgt tcgacgacca tcggaactag gggactcatg
     6661 tgctcgcttc tctttcgctc gttgcgttcg attccttcaa cgacgagctt cgggcccgcc
     6721 cgcggcgcag tctagcaacg gccttctgcc cttctggacg gtctggaagc gcctacgaac
     6781 ccggtgtcca gatttgttga ggagccgctt cgccctcttc cttgccgggc gtccagagct
     6841 tgtcgcgtgc cttcgcctgc tcgaccgaga tcggcttcgc cgactccacg aggatgtcga
     6901 gcgcgcgcct catcgcgatg tcctcgcgca gcgcctcagc gcgaccctgc tgcttcgccc
     6961 ggtccagtga gcgcctgagc gatttctcgc tcggcggctg gccgccctgg cggatggcgg
     7021 cgtcgcgcag tgcgtcgagc agctcgtcgt ccgagacctc gattccctca gcggcaacga
     7081 tcgcggccag cacgctctcg cgcttcagcg cctgctctgc ctgcggctcg gactcggtga
     7141 cgagctcctc ctcttccttg cccatcatct ggaggtacat gcgcgggtcc atgccccggt
     7201 gctggagctg gtgcgccgtc tcgtgccaca tctcatgggc cttcgcgtgg acgagctcat
     7261 gtggcacgtc caccttcgcc tcggccaccg cggcgtcgag cgcggcctcg cggaactcgt
     7321 tctcgatcgt gcgctcgtcg gcctggcgca gcttggcctc gatatccgag cgcagctcgt
     7381 cgagcgtgtc gaagccgccg gcctcggcag cgaaatcgtc gtcgagctcg ggcaaccgct
     7441 tctccttgac ctccttgacg gtggtgtcga agaccgcctc attgccggct aggtgctcgg
     7501 cgccgtagtc gtccgggaag gtcacgcgca cctcgcgctg ctcgccggcc gcggcgccga
     7561 cgagttgttc ctcgaacccc gggatgagcc gctcggagcc gagctcgagc gtgtagccgc
     7621 gcgcctcgcc gccctcgaac gggtcgccat cgagcgtgcc cacgaagtcg atcaccacga
     7681 agtcgccgtt ctgtgcctcg cggtcgacgg tctcgagcga cgcctgcgac tcgcgcaagc
     7741 gctcgagctc gcggtcgacg tcctcgttgg cgacctccgg ctcacgccgc ccgacctcga
     7801 ggcccttgta gcttccgagc ttcgcgtctg ggcgcacgcc gacctcgatc gagaacgaca
     7861 gcggcgagcc cttctcgggg tagtcgccca cgtcgagctt gggatcgccg accgtggcga
     7921 gcctcgtatc ggcgagcgcc tcctcgtacc agttcggaag gccgcgccgg atcgcctctt
     7981 cgagcacggc ctcgcgcccg acgcgctgga tcacgacggg gggcggcacc ttgccgcgcc
     8041 ggaacccgga gatgcgcatc tcctgtccga gcgcgcgcgc tgcgctgtcg agctcccgct
     8101 cgacctcggc cgacgggacc tctacctcca cgcgcacgcg tgaatcgccg agctcggtgg
     8161 tcttggtgct gagcgctgcg ggcatggcca gccacgatag cgtgagcgac ttgacgcgcg
     8221 ccctcaccga tcgcggcagg cgagagcgac tcgtggaatg gctctacacg ggtccgctcg
     8281 gacacctcta cggcgtcctc gcggacctcg tgcagctatg ggtgcgctac ggcgtgcgcc
     8341 gcctgcgcct gcgtgtgtcg cggcgctagc cgatgagcag gagtacgaac gacgcgacca
     8401 ccgcgatccc gccgaggatc gcgccggccg tggcgcgtcc gtcgccggtg ccggactcgc
     8461 gagcgccgag cccgagcgcg agcgcggcgg cgcccaccgc gaacggcagc ccggtccaga
     8521 acacgaaacc gacggcgatc gcgaggaccc cgaggatcag cgcggtgcga gacgcccggc
     8581 cggccgcgcg ctcgcgcgga agcagccaga ggaagaggcc cgccatgatc gcaagcacga
     8641 tgatgatcgc gcccgccatc ccgacggcat ctcccgtgct gccgggcaac caaccgaccg
     8701 gcataccgat cagcagcaga aatgtggacg ccagggcgac gttgcggtcg cgctggagcg
     8761 tcgcgtcgga cgccggagcg gcgccggacg gagtaactgg accagctgag aattccatgg
     8821 ctcagaccta cacccgattc ggcgccctgg tcaagtgccg cagaccgctc aagcaccgca
     8881 cataaggaga tacagatgcc cgagcgatat gacgcgatag tgatcggggc cggtccgtcg
     8941 ggcgaggtgg cagcgggcga cctcgccaat cacgggcttc gcgtggcggc ggtggagcgc
     9001 gagctcgtcg gcggcgagtg cggcttctgg gcctgcattc cgtcgaagac gctgctgcga
     9061 cccggcgacg tgctgagcga ggcgggtcag gcgcccggtg cccgcgaggc agtggacggc
     9121 agcctcgacg tccgggccgc gctgagctgg cgcgacttcg aggtgtcgaa ctacgacgac
     9181 caccaccaag ccgactggct gagcgagatg aaggtggaca tcctccgcgg acaggcgcgg
     9241 ctggatggac ccgggcgcgt acgcgtcggt gacgagctct acgagaccga ccgcgtcgta
     9301 gtcgcgaccg gctcggatcc ggtcatcccg ccggtagacg ggctggccga gctcgaggga
     9361 ctgtggacga accgcgaggc gaccgggctg aaggaggtgc ccgagtcgat gctcacgctg
     9421 ggcggcgggc ccgtgggcgt tgagatgggc caggttcttc gccgcatggg cgcgcgcgtg
     9481 acgatcgtcg agcacgaggg gcggctgctg tcgcgcgaag cgcgcccggt tggcgaggcc
     9541 gtggcggagg cgctcgcgtc gcaaggtgtg gagctacgac ttggccgcgc ggcgacggcc
     9601 gcgcggcacg ccgacggcgg ctttgagctc gacctcgacg acggctcgac cgagcgcgcg
     9661 gagaagctgc tcgtcgcgac cggccgcaag cctcggatcg acgatcttgg cctcgagtcc
     9721 gtcgggatcg agccgggcaa gcgcgggatc gaggtggacg agcggatgcg cgccggtgaa
     9781 ggcgtgtggg cgatcggcga cgccaccggc aagttcctct tcacgcacgt gggcaagtac
     9841 caggcgcgga tcgccgtggc cgacattctc ggcaagcctg cacacgcgga ctaccgcgca
     9901 gtccctcgag tggtgttcac cgatccagag gttgcggcgg tcggcgcgac tgagggcgaa
     9961 cgaaccgccc gcgccgagct cgccaacatc gcgcgcacct ccacatacac gcgcgagtac
    10021 gaccagaatc ccggcttcct ggagctggtg gccgacgggg accggctgac gggcgcctac
    10081 gcggtgggcc cccaggcggg cgagtggatg cagcagatca cgcttgccat ccgcgccgag
    10141 atgccgctcg acgtgctgcg cgacgtgatc cagccgttcc cgaccttctc cgaggtggtc
    10201 tacgccgccc tcaagcagct gtaggcgcta cggggtgggc gcgctctgcg cgggcggtgc
    10261 gctgccgccg ttcgatggcg gcggcgtggc ctgcggcgcc ggcgcggttt gggcaggcgg
    10321 ctgggcgggt gcggtctgag ccggcggctc ggccggtgtc gtcggcgtcg gagaggactg
    10381 cgggttctgc tgcggctgcg tctgctcggg ctgggtcgtc gtcttctgac ggtcgcggct
    10441 ggcactcgag tggccgccgc cgaagagcgt tgtccggctg ccggtggtga cggagccgcc
    10501 gaacaccagc tctgggacgg tgagcacgag agcaccgatc acgaatgcga cgagcccggt
    10561 tgcgatcgcc gccttcacgt ggaggttgcg gcgggggcgg tagatcgtca cgccgccgcc
    10621 gtttccgctg gacgagccgc cgttccgctc gaacgtgcgc tgctgtggac gcgatgccgt
    10681 cagcgttccg cctcgagcgc gcccgcgggc gggctgtcgc gattcggcca ccggcgcgag
    10741 ccgcttcacg gccggcgcga gctgcgtgac cttgcgggcc ggccgccggt aggcctcgct
    10801 cgcgagcgcc acgatcaccg gcgtcatggc ggcggagatc accgtcccgc ccttccagaa
    10861 gtgcgaggtg atgagcatcg ccgtcacgga ggcgaggctt gccacgatga tcgtgtcgag
    10921 cgacacgccg ccgctcttgg cttgatctct gtcggccatg cgctgctaga gcgcgggcgg
    10981 tgccgtcgcc gagggcgcgg ccacagcggc cgttgagcac gtcggggctt gcaccccacg
    11041 caggttagta aacgtcagga ccgatcgtgg gcctggcccc cggcaggcca ctgaagggcc
    11101 cgcgctacca cagccagacc cacgctcggt ccgcgcacgg acgtgtccgc gcgctatttc
    11161 ttgaaggaga ctccgaggac cttcgaaccc gcgccgttgt ggtccgcgtc gccgagccac
    11221 tgagccacga agatcgtgtc ccggcgcacc ttccagacgg tgctgaagct gtcgttcgag
    11281 gcgacctgaa ctaccttgtg gacccagccg cccttcaccg accgcgcgga gacgacgacc
    11341 tgctccccgc cctgcgcgcc gggcagcttg ccggtcacct tgatcgtcga ggccttggtg
    11401 agcttcgtct tcttcgtctt cagccggagc gacgacgccg tgcccaggtc tccaccggtg
    11461 cgggtgaaga acatgttgtt ggcgttgtcc cggacgtaat ccgttccacc actcgagacg
    11521 agcgacaaat cgttgatcga gttccggctc accagctgcg gccgccaggt gtggccggcg
    11581 tcagaggtcc gcaggagcag tccccccgtc tggaagccga agcggcggat cacgaggaag
    11641 ccggtcttcg tgctgctgaa gtcgatcccg acgacatcga gcgtgccggt acccacgagc
    11701 tgcgtccact tcgcgccgcg gttcttcgtg aaccagaggc gtccgttgct ctccagcacg
    11761 tagccagcct tgctcgtcgt gaaatcgagg tcgttgacgc ggaagcgctt ctgcggcagc
    11821 ttcagctgct gccatttcgc gccgccgttc gtggagaagt acgccgcctt cgacccgtag
    11881 acgaagaccg tgctacccac gtggtccacc gagtcgaggt tggcgttccg gaccggcttc
    11941 gcgttgatgc ggctgaacgc ctgcccaccg tcggtggagc ggcgcacgcc ctgcggtccc
    12001 acgagcagca cccggttcgc atcgagcgcg agcaccgacg ggggcgtcgc cgacgtgccg
    12061 gtgtcgagca gcgcccagct accgccgcca ttggtggtct tgaacagcga gcccgagctg
    12121 tcgagcgcgt agccgttcga gccatccggg aaggacacgt cgacgacatc ctccgatgtc
    12181 ggcacgccgc cgccgctcca tgactggccg ccgttcgtgg tgcgagcgag cgacccgttc
    12241 gccccgggcg cccaggcgac ggtgcccgac gtggccacga gctcgaagaa cgagccggcg
    12301 agccgtgctc cgatcggtga gaagttctgc ccgccgtcgt cggacgccac tgtcgacccc
    12361 gcctcgccga cggcgacaac gcgtgtcggt gacgcgaacc ccgccacgta gaccctctgc
    12421 gacgacggcg agacctcgct gccggtacct ccgccgtcgg tcgtgcgcac gagcgtggcg
    12481 ccgtcgacgg tcgcgaggat gcacgtcgag ccgacgcagc ggatcgagcc gaagttgccg
    12541 ccgctcacgc cgccgagggg cttcgccgcc caattgtcgc cgccgtcggt ggtcgcgacc
    12601 agcgtcttgc cgtcgccgac cgcgtagccg gtgcccgcgt tctcgaacca aagggcgttc
    12661 aggttcgccc cggcggtcgt gacgagcgtc catgccgcac cgccgttcgt ggtccggtag
    12721 atcttgccgc cgccggctcc gcgcgtgatc acgtagccca cggtcgggct cgtgaagaag
    12781 atgtccgcgg gcgtcgcggg ggtgccggcg ccggttgcag gtgtgcccgg cactgccgtc
    12841 tggcgcgaga acgtctggcc gccgtcggcg ctcgacagca ccgtcccgtc ctggagcaac
    12901 aggaaccccg cctgcggcgt cgggaacgag aatgaggaga tctgcgccgg gcagttgatg
    12961 tcgctgttcg agaacggcag gcgatggaac gtctggcctc cgtcgtccga gcggcgcgca
    13021 gtgcagccgc cggcgatcac gatggtgttc gaatcgacga cgctcacgcg cttgaggtcg
    13081 gcggtgatgc cggtcggaac gcccgaccag gtcgcacccg cgtcgtcggt gcgcagcagc
    13141 gtgccgaagt cacccgcagc gaatccgcgg ccgcccgcga agtcgagcgc acggagcgtg
    13201 ttgccctgcg gcagcgggtt gccccagctc cagccggaat gccccactga aacggacgca
    13261 cctgccgacg gcgcgatgaa tgcgagcccc agtgcagtgg ctgccacgga cgcaacgatg
    13321 agacgcttga tcatgcgtgc cgaacctttc gtgaatggcg cgtcgcgcca gggtcgatct
    13381 gacgggtacg aaccgtttcc gtctgggtta gttgcgcagg gtgtcagagc cgctggacgg
    13441 ctccgtccct atggcacgca gatgcccgtc gagtcggcga cctggccggc gccgggcacg
    13501 tagaggctgc tctggccgca gcgcaccggg agcagccgcg ccgccgcctc gcggatctga
    13561 gtcaggtgag cccgcgcacg ccggcgcgcc atgcgctttc gtgccgcgag cacgcgccgc
    13621 tgatggcgcg tgagcgtgga cctcgacgcg ccgaccgcgg ggagcggatc caggatcggc
    13681 ccgaggacgg gatcgacgat cggcccgagc acgggcaaac cgctgagcag ctggtcgatc
    13741 aggctctggc cgcctccgct ggtcccgccc ccgccgcccg agttgtcgaa ctggtcgccg
    13801 aagccgctcg ccacgttctc gaacacgagc cggttcgagc ccttcttgta gtcgaagggc
    13861 caggattcga tcgtgcagta gatctcctgg cagccggaga acagggtcag atggccggag
    13921 cgcgacgccc tgaagctggc gtcggtgcgc acgcgcagcg ccaccgccga gacccgggtg
    13981 acggtgacct tgtacgtgcc gcggaggccg ctcgcgtcat cgcggaaggt cttggagcgg
    14041 accgccgcgc ccgccggctg taccgcgcac acgagcgcca cggtgacaag gcccgccgag
    14101 aggacgatct tcaacccgct cccacgcatg ctgctcctcc cgcctggctt gcctccggcg
    14161 gccatcctac gcccgaagtg cgcctaccgc gacgtttccg cgctagcggc ggcgctcgac
    14221 ggcgcgctgc aggcgcgcca gcgtgtcgtc ccactcgccg ccgacagcag cgatccaaga
    14281 ggccgcatcc gcgagcgggg caggcgtcat ctcgtagcgc gcctcgcggc cctcgcgcgt
    14341 gcgctcgacg agccccgccc ggccgagcac ggcgaggtgc ttctccaccg cctggcgcgt
    14401 gatcggcagt tcggcggcga gccgcgacga ggtctcgggc gcgccgcggg agagcgcctc
    14461 gagcagcgcg cgccgtgttg ggtccgcgag ggccgtgaag acctcgtcgg cggctgcctg
    14521 ccgcatctgc gcctaggcga gcgccatcga cgacaccgac gccagcgcgt tgagcgccgg
    14581 gccccaggta accggaaccc gaaggccgcg ctcgacgacc agcacccgcg tgcccgtggg
    14641 aactgcctcg agcgtgaaga ccacgctggt ctccccctgc ccctcgcgat gccagcggaa
    14701 tgcgaggcgc tgctcctcct cggcctcgtc gacctcgagg tcgcgctcgt ctccgtcctc
    14761 gaagagccag tcgcgcagga gcgccggctc ggacagggcc tcccacacct cgccgggcgt
    14821 ggcttcgatc gtgatctctc gctccacctt catctcgggc gccccttccg gactcgctgg
    14881 ctggccattg cgcaacccaa tagttgcaga ttcgctacgc tgcaaccaaa cgcaacctca
    14941 cggttgcact tccctgaaag gaccggccat gcccctgatc ccgatggttg tggaacagag
    15001 cgcccgcggc gagcgctcct tcgacatcta ctcgcgcctg ctcggcgagc gaatcgtctt
    15061 cctcggccag caggtcgacg acgagatcgc gaacctggtt gtggcgcagc tgctgcacct
    15121 cgagtccgag gaccccgaca aggacatctc gctctacgtg aactcgccgg gcggcagcgt
    15181 gtacgcggga ctcgcgatct acgacacgat gcagttcatc aggccggacg tgcagacgat
    15241 ctgcttcggc gtcggcatgt cgatgggcgc gctgatcctc gccggcggag cgcccggcaa
    15301 gcgcatggcg ctccgcaaca gcaggatcct gatccatcag ccctcgggcg gcttccaggg
    15361 cacctccgcc gacatcgaga tccacgcgcg cgaggtgatc gagctgcgcc gccgcctcga
    15421 cgagatctac gcgcaccaca cgggccagcc gctggagcag gtgcacgacg acatggaccg
    15481 cgaccgcttc ttcaccgcgg agcaggccgc cgagtacggg ctcgtcgatc gcgtgatcga
    15541 gtcccacgag ctgagacgtt cggcttacgg cttccggccg aacggagctg agggctgagc
    15601 gcagttctca tcccaacggg gtgaaccgcg gcgggcacgg tggcgcgaga atcgggcgcg
    15661 cgtggccacg cactcgcgca cgcccggcag tgagcacctg gcgcggatcg agcgcctccg
    15721 ccgcttgccg cccgtcgcgt tcgccgagca ctgcggtcgc aaggccttcg agatgggagc
    15781 gatcgaccgc gcgatcgtgc tcgcgtcgca ggcgtacgtt gcgatcttcc cgttcctgat
    15841 cattgtgggc tcgatcgcac cgcggcgggg tgcgaaggac acggcagacg gcctgatcga
    15901 ccgcttcggg ctgacgggat cgaccgccca gtcagtccgt gatctgttca gctcgcccac
    15961 cgacgtgaag aacgccctga gctgggcggg cgtaatcgtg ttgctcctgt ccacgctcag
    16021 cttcacgcgc tcgctgcagc gggtgtacga gctgtgtttc tcactgcccg cggcgggcgc
    16081 ccgcaacacc aagcgtgggc tgctctggct cggcggcctg gccgtatacg gctgggtcgg
    16141 cacgctgatc gccctcgtgc gcgccgggcc gctccgtaac gcgctcgccg tggtcctctc
    16201 gctcgcttgg tcgttcgtct tcttcacgtg gacgccctac gtgctcacgg gctaccgcgt
    16261 gccttggcgg cgtctggtgc ccatcggctt cgctacgacg accggcctcg cgttcttcgt
    16321 tctcgcgtct ggcgtctata tgccgcacct gatctcgcgc cagtcacatc gctacgggct
    16381 gatcggcgtg gcgttcgccc tgctctcgtg ggtcctcgtg ggcgcgttcg tgatagtcgc
    16441 ggcagcggtg atcgggtccg aggtcagcga aacgcccagg cgtatgtatc gccggctgcg
    16501 cggaagatga gcggtgggaa gtcgttcggc tcccaggact cgggcgtccg gtcgaggatg
    16561 aagccggtcg cgagagcggc caagtggtcg acgagctcct gttccgatgc gaagcgccgc
    16621 ggatcgaccg gctcgtgaag acgcagacgc ggatgccggc agccgccttc tgagactgaa
    16681 ggcacgatga gcccgccggt ttcgcgcgcg aggcgcgcga tcccgctacc tacgcgcgct
    16741 cgcttgccaa gcaatgtggt ctcggtgcca ccgggcacgt caaagtggaa gaggcacacc
    16801 tcgccgcgtt cgagcagcgc ccggaagacc tcgaaggagc cgccgcgggg gacccagcgc
    16861 ccgccgttgt cctcgaacac ccggcgctcc agcagtgcca ggagaccgtc ccgcccctcg
    16921 tacacgtcgg cgtagagcca gtccgctccg gccatgtaca agcggtagcc cttcacggac
    16981 agcggcgccg acgtgccgcg cgggggaccg aagtggaggc ccatgagaag cacgccgcgc
    17041 ccgcctgcct gagcggcttc gaggtgctcg attccgacga ctggatagcg gcgcgcgacc
    17101 cagggccgaa gcttcagctc gcgccgcagc gcctgctcga cgatctgccg gcgagcgatc
    17161 cgctcggcct cgcgctctcg gccggtgcct tcgagcatga gcgagacctg cgcgatcgcg
    17221 cgcgaccgcc gctcccctga gaagcgccat agcagccgcg cccgcagcgc ggctagtccg
    17281 agcgcgagcc agtcgggaag cagccgatgg acgcgcggcg aggtgtacag ccgctcgaac
    17341 agcggcacgc gaccgcgcac cgcctgatgc gcgctcgggc ggtcgatcgc gtcaagcgta
    17401 tgccccaggt cggcgccgtc ttccacggcg cgaatcccca tccgcgccac ggtaatgccg
    17461 cggggccgcc cacgccagcc ccgatgtcag gtcttgatcc gcttctcgaa ccagtgatgt
    17521 gcgtagggct cgtcgttgaa cggggcaacc tcggcgtagc ccgccgagcg gtagagcgcg
    17581 atggcctgcg tgagcgtgcg gttggtctcg agtcgcacga ctcgacttcc gctctccacg
    17641 gccagcgtct cgagctcgcc gagcaggcga cgaccgagcc cgaacccgcg agccgactcg
    17701 gccacccaca tccgcttgat ctccgtcggt tccccagggt ggaacttcag cgcgccgcat
    17761 ccgaccggct ccgaccccag cgaggccacg agcagcactc cggccggcgg gcgaagctcg
    17821 cgcgcgtccg cagagatgct gcgcttcggg tcaaagccgt cgtcgaacct gcgatcgagc
    17881 tcgctcacgt actcccgcag gcagaactga gcctgcgggt ccgtggggtc gcgcggtgcg
    17941 atctcaacca tcccgagcgt gagcaagcgc tcgacttcgc ccatcgcgtt cacgagccgc
    18001 tcgcgctgcg acgcgctcag aggtgcgagg aacgactcgg cgaggtcatc gctgcggcgg
    18061 tcgagaacgt tccgctcctt cacgcctgcg gcggtcagct gcgccgtgta cacgcgcctg
    18121 tccccgtcac agcgctccac gacgatcagc ccggcctgct cgagcgaccg cagcaggcgg
    18181 ctgaggtagc ccgagtcgag accaagccgc gaccgcagcg tgcggacgtc ggatccgtcc
    18241 gggccgatct cccacaggac gcgcgcttcg ccaagcggac gatctcgggc gaggaagtga
    18301 tcctcgagcg cgcccgcacg gcgtgtcacg acgcggttga accggcgcac ctgcaccagc
    18361 tggtcgtggc tcattagctg accttagtca gctaactccc gcatagcaag acaagcttca
    18421 ccactcggtc acagccggac gacatctggc gtttgcaacc cgtcgccggc ggtggcctgt
    18481 cccgctgcga ct
//
LOCUS       DASVXS010000290         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_698884, whole genome shotgun sequence.
ACCESSION   DASVXS010000290 DASVXS010000000
VERSION     DASVXS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698884"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VF752_16295"
     CDS             <1..746
                     /locus_tag="VF752_16295"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8857156.1"
                     /translation="RGGVLINHAYATAADLHVGDTIQLRGPGGVRGARVEGLLHGVAG
                     FNGLGMQMSLASMRSIYGATIDNLVLVNASSDAARDPLARRIDAYLSKRHPNLESLST
                     AEVKDQIKTQINQQFNLFNAIIAIAVIVSLLGVVNTLAMSVMERTREIGVLRAVGSSR
                     LLVRSTMLDESLLITLAGAIAGIATGLLIAFVWVSGLDSVLPGIGFRFPVAATVSVTV
                     AAIVLGVLAAILPARRAARLNVIEALSYE"
     gene            771..>1513
                     /locus_tag="VF752_16300"
     CDS             771..>1513
                     /locus_tag="VF752_16300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8857157.1"
                     /translation="MGHTALETQRLERLLDTGRDLVSELDLEALLNRLLEVARELTGA
                     RYAALGILDERREELERFVTAGIDAETHAAIGDLPRGRGVLGVLISDPRPLRLARVGD
                     HPRSYGFPTAHPRMDTFLGVPIKIRGEAWGNLYLTEKEGGAEFTEEDEESAVVLAEWA
                     AIAIENARLYERAEGRRVELERAVAELTATNEIARAIGAETDLSRVLELIVKRGRAQV
                     DARAMLISLREGSELVVRASAGEVPPELIG"
BASE COUNT          233 a          517 c          533 g          230 t
ORIGIN      
        1 cccgcggcgg cgtgctcatc aaccacgcgt acgccaccgc ggccgacctg cacgttgggg
       61 acacgatcca gctgcgtggc ccaggcggcg tccgcggcgc ccgcgtcgag ggactgctgc
      121 acggggtcgc gggcttcaac ggcctgggca tgcagatgtc gctcgcgagt atgcgcagca
      181 tctatggggc cacgatcgac aacctcgtgc tcgtcaacgc cagctccgac gcagcgcgcg
      241 acccgcttgc gaggcgcatc gacgcgtacc tgtcgaagcg ccacccgaac ctcgagtcgc
      301 tctcgacggc cgaggtgaag gatcagatca agacgcagat caaccagcag ttcaacctgt
      361 tcaacgcgat catcgcgatc gccgtgatcg tgtcgctgct cggtgtggtg aacacgctcg
      421 cgatgtcggt gatggagcgc acgcgcgaga tcggcgtgct gcgcgcggtc ggctcgtcgc
      481 ggctgctcgt gcgcagcacg atgctcgacg agagcctgct catcacgctc gccggcgcga
      541 tcgccggaat cgccaccgga ctgctcatcg cgttcgtgtg ggtctccggt ctcgactcgg
      601 tgctgcccgg gatcggcttc cgcttcccgg tcgcggcaac cgtctcggtg acagtcgcgg
      661 ccatcgtgct cggcgtgctg gcggcgatcc tccccgcacg cagagctgcg cgcctgaacg
      721 tgatcgaggc gctcagctac gagtagttcg cgtgacccta agatgcccat gtgggtcaca
      781 ccgcgctgga gactcagcgc ctcgagcggc tgctggacac ggggagggac ctcgtctcag
      841 agctcgactt ggaggcgctt ctgaaccgcc tgctcgaggt tgcacgggag ctcacgggtg
      901 cccgttacgc ggcgctcggc atcctcgacg agcggcgcga ggagctcgag cgcttcgtca
      961 ccgccgggat cgacgccgag acccacgcgg cgatcggcga cctgccgcgc gggcgcggcg
     1021 tccttggcgt gctcatcagc gacccgcggc cgctgaggct cgcgagggtg ggggaccacc
     1081 cgcgctccta cggctttccc accgcccatc cgcggatgga cacgttcctc ggcgtgccga
     1141 tcaagattcg cggcgaggcc tggggcaacc tctacctcac cgagaaagag ggcggcgccg
     1201 aattcaccga ggaggacgag gagtccgcgg tcgtgctcgc cgagtgggcc gcgatcgcca
     1261 tcgagaacgc gcggctgtac gagcgagccg agggccggcg agttgagctc gagagggccg
     1321 tcgccgaact cacggccacc aacgagatag cgcgcgcgat cggcgccgag accgacttgt
     1381 cgcgagtgct cgagctgatc gtcaagcgtg ggcgcgccca ggtcgacgcc cgcgcgatgc
     1441 tgatctccct gcgcgagggc agcgagctcg tggtgcgggc aagcgcggga gaggtgccgc
     1501 ccgagctgat cgg
//
LOCUS       DASVXS010000291         7993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1265512, whole genome shotgun sequence.
ACCESSION   DASVXS010000291 DASVXS010000000
VERSION     DASVXS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265512"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VF752_16305"
     CDS             <1..997
                     /locus_tag="VF752_16305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8857158.1"
                     /translation="QIAPWNYPLMMAIWKIGPALATGNTVVLKPSENTPLSTARLAEI
                     AAEFLPKGVLNVVHGHGQPAGEAIVKHPDVRLVSITGSVESGKKVAAAAAEQLKRVHL
                     ELGGKAPVLIFDDADIEAAIEGVKIGGYFNSGQDCTAASRVIAGPKVYDDFVSGLSDA
                     AKSLNLDDPMSADSEMGPVISDAQRQRIQGFLDRAPEYAEVVTGGHAPDRGGFWFEPT
                     VVAGLRQDDEMVQREVFGPVVSVQRFSSDEEAFEWANGVEYGLAASVWTRDVGRAMTA
                     AKRLMFGTVWVNDHITMASEMPHGGFKMSGYGKDMSAYALEDYTELKHVMVSLDY"
     gene            complement(1163..1774)
                     /locus_tag="VF752_16310"
     CDS             complement(1163..1774)
                     /locus_tag="VF752_16310"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX8857159.1"
                     /translation="MERAAQPEKVSHQLERWLQSEGDKTVGSLIALFEEKSFAILFVV
                     LLGVPALPLPTGGVTHVFEIIAMLLALQLIVGRDMIWLPERWRKVELAGRGQQRFING
                     LMKTIRWLERFSKPRARFLFDHRLSNSVFGVLVFAGSIGAFLAPPFSGLDTLPALGTV
                     IISLGVLLEDIVFVAAGILVAAGGVILEIVLGRAAVKGIGSLF"
     gene            complement(1786..2661)
                     /locus_tag="VF752_16315"
     CDS             complement(1786..2661)
                     /locus_tag="VF752_16315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8857160.1"
                     /translation="MRIVGDMVNDKQEIHLSSGPISYTDSGSGEPIVLLHGLLQDGRL
                     WRKMVPLLEPHARVVVPDLPLGVHKKAMDPGADVSPKGVARLVGEMIDKLGLERPTIV
                     GVDTGGALAQLLIAERPEIAGRLVLGPSDAFENFFPPLFRPLQYVARVPGGLYAFVQP
                     LRIRALRNLPIAFGWLTKRGFPPEVTDTWLNAFFGDKAVRRDAVRFLRAIDSGDTLDA
                     AARLSSFHGPALIAWAEEEKVFPRGDAERLAKVLPNSRLEFVPDSYTFLPEDNPEAFA
                     KLILEFMREGAPAAA"
     gene            2679..3329
                     /locus_tag="VF752_16320"
     CDS             2679..3329
                     /locus_tag="VF752_16320"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8857161.1"
                     /translation="MHTDSTHVNRKAAQSAATRDRLVEVARRLFAERGYAAVGTEEIV
                     RDAGVTRGALYHQFTDKRDLFRAVFEAVEAEVAQRVAESALSRNDPIDQLRVGFTFWL
                     DACAEPETQRIVLIDAPAVLGWEEWREIGERYGLGIVVAALQGAMDAGAIERQPVHAL
                     AHVVMGALDEAALYVARADDAPAARAEMESVLGRMVETMRPTSRTSRRSAPAKGLA"
     gene            complement(3280..3900)
                     /locus_tag="VF752_16325"
     CDS             complement(3280..3900)
                     /locus_tag="VF752_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857162.1"
                     /translation="MHAGSERFWPRRLLWRFRGAWTWPAFAIFTIADGVLLHELPPQS
                     TGIGLVPGLIIASFGNLFLIGLVAPWLARRLVARPTASPRERRVPPEVVHDRTATILL
                     VCGTLGVIAAGLASRPAVITETNATQANAKAVRDYVLTRGSAEEKRNVQLGKADSIRL
                     AKGYFRTCIPRDNSRLAFCYFVDTNRKPPTIKRDPSQVPNADSFVK"
     gene            4074..5141
                     /locus_tag="VF752_16330"
     CDS             4074..5141
                     /locus_tag="VF752_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX8857163.1"
                     /translation="MATTTARPAAIEDGGGASVDLTGDDRRALLRYMLLMRATEERAL
                     TLYRQGKVPGSFYDGRGQEAVSVGAAFTLGPRDRTCILHRDLGAHLIRGVKPGRVLAQ
                     MMGRAGGVTAGKDGNMHFADHQLGCVGMVSMLPDMALVTCGLAIAFQMRREPRVAMTW
                     FGEGSTANGQWHEAMNVAGIRKLPVVFILENNQFAYSTPNDHEFAVDPVKRAEVYGFP
                     GVKVDGNDVEAVFFAAAQAVERARSGGGPTLIEAETMRMHGHGAHDDMSYVPKEMVEH
                     WTARDPIDNYERKLREEGFDTDEIRESVKGELDAETEWALAQPMPEPHTALEGVFATE
                     DPVLGDGNAPWSRWQREAANA"
     gene            5134..6102
                     /locus_tag="VF752_16335"
     CDS             5134..6102
                     /locus_tag="VF752_16335"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8857164.1"
                     /translation="MPEITYLQAISDGLREEMRHDESVFCLGEDIGAFGGAFKVTDGF
                     IEEFGADRVWDTPLAENAIIGTAVGAAAEGLRPVCEMQFADFISCGFDQLVNVAAKMH
                     YRQGLAVPMTVRLPSGGGFSGGPFHSQNPEAWFLQSPGLKVVAPSTPEDAKGLLSAAI
                     RDPNPVVYLEHKHLYRRAKGEVAEGRFEIPLGEARVAREGTDLSVITYGAMVQLALDA
                     TEDLDGASVEVLDLRSLVPLDTGAILESVRKTSKVVVLHEATRSCGVGAEVSALIAQE
                     AFEDLDGPVVRVTAPDTPVPFSPPLERAVLPQPDDVRRACLELLAY"
     gene            6083..7381
                     /locus_tag="VF752_16340"
     CDS             6083..7381
                     /locus_tag="VF752_16340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX8857165.1"
                     /translation="MSSSPTSAQTTVDVVMPQMGVSVAEGTVAEWRKRVGDWVEADET
                     ILEISTDKIETEVPSPASGRVTEVLVDVGSTVDVGTVLARIDTGAKPGEPHADEHAPQ
                     AGPAVAEPAAAEAGPLPEKAHPPEPAEHGGNGHGPPISPVVRRIAEEHQIDLSQIEGS
                     GRRGRVTKRDVLAFLERRDDAAAERPLHIESPYRDEEPAPVQEPAPEREAAPAVGGEP
                     LSLMRQKIGEHMRRSLDTAAHCTTIVEADMSRIEAARKSGREKLTYLPFVARAVIAAL
                     REYPMLNATLEGDRLTVHEEVNLGVAVSLGEDGLIVPVVHNAHELSHEGLAARIKDLA
                     ERARAKRLEPDEVRGGTFTITNPGAYGALLATPIINQPQVAILDLEAVVKRPVVVDGD
                     SIAIRPMTYLCMSWDHRALDGALSAQFLSAVRRHIEEWSG"
     gene            7374..>7993
                     /gene="lipB"
                     /locus_tag="VF752_16345"
     CDS             7374..>7993
                     /gene="lipB"
                     /locus_tag="VF752_16345"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX8857166.1"
                     /translation="MAEPLSELWVARIPGLVPYAEGVALQERVRALRQEDRIPDALLL
                     LEHSPVFTRGRRTEAADLPFGDDWYRERGVEVHDTDRGGRVTYHGPGQLVGYPIMRID
                     DVLEYVRTMERAMIAALSDEGIDAGLREGLTGVWVENRKIGSIGVHVSRGVTTHGFAV
                     NVENDLEPFEWVVACGIGGVQMTSVRQEGGEGIDCFRKRMAWRFAEA"
BASE COUNT         1303 a         2694 c         2838 g         1158 t
ORIGIN      
        1 gcagatcgcc ccgtggaact acccgctgat gatggccatc tggaagatcg gcccggcgct
       61 cgcgaccggc aacaccgtgg tgctgaagcc gtcggagaac acgccgcttt cgacggcgag
      121 gctcgccgag atcgccgccg agttcctgcc taagggcgtc ctgaacgtgg tccacggcca
      181 cggccagcca gcgggcgagg cgattgtgaa gcaccccgac gtccggctcg tgtcgatcac
      241 gggctccgtc gagagcggca agaaggtggc ggcggccgcg gccgagcagc tcaagcgcgt
      301 ccacctcgag ctcggcggca aggcgccggt gctgatcttc gacgacgccg acatcgaggc
      361 ggccatcgag ggcgtgaaga tcggcggcta cttcaactcc ggccaggact gcaccgccgc
      421 ctcgcgcgtg atcgcgggcc cgaaggtgta cgacgacttc gtgtcggggc tctccgatgc
      481 ggccaagtcg ctgaacctgg acgaccccat gtcagccgac tcagagatgg gccccgtgat
      541 ctcggatgcc cagcgccagc ggatccaggg cttcctcgat cgcgcgcccg agtacgccga
      601 ggtggtcacc ggggggcacg cgcccgaccg gggcggtttc tggttcgagc cgaccgtggt
      661 cgccggcctg cgccaggacg acgagatggt gcagcgcgag gtcttcggac cggtcgtctc
      721 agtgcagcgc ttctcctccg acgaggaggc gttcgagtgg gcgaacggcg tggagtacgg
      781 cctcgcggcg tcggtctgga cgcgcgacgt gggccgcgcg atgaccgccg ccaagcgcct
      841 gatgttcggc accgtgtggg tgaacgacca catcacgatg gcctccgaga tgccgcacgg
      901 cggcttcaag atgtcgggct acggcaaaga catgagcgcc tatgcgctcg aggattacac
      961 ggagctaaag cacgtaatgg tctcgctcga ctactaatcc tcgagctact caggcgcggc
     1021 gcctgcaaac ctgcgcagct cggcgtcctc gggcgctcgg cgtgcccagc acgccttcgg
     1081 ccctgcgtcc ttgatctgct tggttttcgc cgccgttcca gggctcaagg ggccgcgctg
     1141 cgcgcggccc ctttcttctg tctcagaaaa ggcttcctat gcccttcacc gccgccctgc
     1201 ccagcacgat ctccaggatc acgccgcctg cggcgaccag gatcccggcg gcgacgaaca
     1261 cgatgtcctc gaggagcacg ccgagcgaga tgatcaccgt gccgagtgcg ggcaacgtgt
     1321 cgaggcccga gaacggcggc gcgaggaagg caccgatcga gccggcgaag acgagcacgc
     1381 cgaacaccga gttgctcagg cgatggtcga agagaaagcg ggcgcgcggc ttcgagaagc
     1441 gctcgagcca gcgaatcgtc ttcatgaggc cgttgatgaa acgctgctga ccccgaccgg
     1501 ccaactcgac cttgcgccac cgctccggga gccagatcat gtcgcggccc acgatcagct
     1561 gcagggcgag gagcatcgcg atgatttcga atacgtgagt tactccgccc gtggggagcg
     1621 ggagtgccgg cacgccgagc agcaccacga acaggatcgc gaagctcttc tcctcgaaga
     1681 gcgcgatcag gcttccgacg gtcttgtcgc cctcgctctg cagccagcgt tcgagctgat
     1741 ggctgacctt ctccggctga gcggcgcgtt ccactcccgc gcaggctacg cagcggcggg
     1801 cgcgccctct ctcatgaact ccaggatcag cttcgcgaac gcctcggggt tgtcctccgg
     1861 aaggaacgtg tagctgtccg gtacgaactc gagccgcgag ttcggcagca ccttcgccag
     1921 ccgctcggcg tcgccgcgcg ggaacacctt ctcctcctcg gcccaggcga tcagcgcggg
     1981 cccgtggaag gagctcaggc gcgccgccgc gtcgagcgtg tcaccggagt cgatcgcgcg
     2041 caggaagcgg accgcgtcgc ggcgcaccgc cttgtcgccg aagaacgcgt tcagccacgt
     2101 gtcggtcact tcgggcggaa aaccgcgctt cgtgagccag ccgaaagcga tcggcaggtt
     2161 gcgcagcgcc cggatccgga ggggctggac gaacgcgtag agcccgccgg gcacgcgcgc
     2221 cacatactgg agcgggcgga agagcggcgg gaagaaattc tcgaacgcgt ccgacggccc
     2281 gagcacgagc cgccctgcga tctcgggccg ctcggcgatc agcagctgcg cgagcgcgcc
     2341 gcctgtgtcc acgcccacga tcgtcggccg ctcgaggccg agcttgtcga tcatctcgcc
     2401 cactagccgc gccacgccct ttggcgacac atcggcgccg ggatccatcg ccttcttgtg
     2461 gacgccgagc gggaggtcag gcacgaccac ccgtgcgtgc ggttcgagca gcggcaccat
     2521 cttccgccac aggcgcccgt cctgaagcaa cccgtggagc aggacgatcg gctcgccgct
     2581 gccgctgtcc gtgtagctga tcggaccgga gctgagatgg atctcttgct tgtcgttcac
     2641 catgtctcct acgattcgca tacaggctgt acgtatttat gcatacagac agtacgcatg
     2701 tcaatcggaa ggccgcgcag tcggccgcca cccgcgaccg cctcgtcgag gtcgctcggc
     2761 ggctgttcgc ggagcgaggt tacgccgcgg tgggcaccga agagatcgtg cgcgacgcgg
     2821 gagtcacgcg cggcgcgctc taccaccagt tcaccgacaa gcgcgacctc ttccgcgcgg
     2881 tgttcgaggc ggtcgaggcg gaggtggcgc agagggtggc cgagagcgcg ctctcccgca
     2941 acgacccgat cgaccagctg cgtgtcggct tcaccttctg gctcgatgcg tgcgccgagc
     3001 cggagacgca acgcatcgtg ctgatcgacg cgccggccgt gctcggctgg gaggagtggc
     3061 gcgagatcgg cgagcggtac ggcctgggca tagtggtcgc ggcgctgcag ggcgcgatgg
     3121 acgcaggggc catcgaacgt cagccggtcc acgcgctggc acacgtcgtc atgggagcgc
     3181 ttgacgaggc cgcgctctac gtggcgcgcg cggacgacgc gccggcagcg cgcgccgaga
     3241 tggagtccgt cctcggacgc atggtcgaga cgatgcgccc tacttcacga acgagtcggc
     3301 gttcggcacc tgcgaagggt ctcgcttgat cgtcggcggc ttgcggttgg tgtcgacgaa
     3361 gtagcagaac gcgagccgcg agttgtcgcg cggtatgcac gtgcggaagt agcccttcgc
     3421 cagcctgatc gagtcggcct tcccgagctg aacgttgcgc ttctcctcgg cgctgccgcg
     3481 ggtgagcacg taatcgcgca ccgccttggc gttcgcctgc gtggcgttcg tctccgtgat
     3541 cacggcaggc cgtgaagcga gcccggcggc gatcacaccg agcgtcccgc acacgagcag
     3601 gatggtcgcg gtgcggtcgt gcaccacctc cggcggcacg cgccgctcgc gcggcgaagc
     3661 cgtcgggcgc gcgaccaggc ggcgcgctag ccatggcgcc accaggccga tcaggaacag
     3721 gttgccgaac gaggcgatga tcagccccgg cacgagcccg atacccgtgc tctgcggcgg
     3781 aagctcgtgg agcaggaccc cgtcggcgat cgtgaagatc gcgaacgccg gccacgtcca
     3841 ggcgccgcga aagcgccaca gaagccggcg aggccagaag cgctcactgc ctgcgtgcat
     3901 cgactcgcag ggtacccgcg gcgctgcaac cgaacgaacg tccagtgatt tgcgccgtgc
     3961 gccagggtac gcttgggata catacccgcg cgccttcgcg gagaccccgc ttggtgcgtt
     4021 caacacggca gtccgtgggc ggagaccccg gcccgggctt cggagggagg aatatggcga
     4081 cgacgaccgc gagacccgcg gcgatcgagg atggcggggg agccagtgtc gacctgaccg
     4141 gcgacgaccg tcgcgcgctg ctgcgataca tgctgctgat gcgcgcgacc gaggagcgtg
     4201 cgctcacgtt gtaccggcag ggcaaggtgc cagggtcgtt ctacgacggt cgtggacagg
     4261 aggctgtatc ggtcggcgcg gcgttcacgc tcgggccgcg tgaccgcacc tgcatcctgc
     4321 accgcgacct cggtgcgcac ctgatccggg gcgtaaagcc cggccgagtg ctcgctcaga
     4381 tgatgggccg tgccggcggc gtgaccgccg gcaaggacgg caacatgcac ttcgccgacc
     4441 accagctcgg atgcgtcggc atggtctcga tgctgcccga catggcgctc gtgacctgcg
     4501 gactggccat cgcgttccag atgaggcgcg agccgcgcgt ggcaatgacg tggttcggcg
     4561 agggctccac agccaacggc cagtggcacg aggcgatgaa cgtggccggg atccgcaagc
     4621 tgccggtggt gttcatcctc gaaaacaacc agttcgcgta ctcgaccccg aacgatcacg
     4681 agttcgcggt cgatcccgtg aagcgcgcag aggtgtacgg cttcccgggc gtgaaggtgg
     4741 acggcaacga cgtcgaggcc gtcttcttcg ccgccgccca ggcggtggag cgcgcacgca
     4801 gcggcggtgg gccaacactg atcgaggccg agaccatgcg catgcatggt cacggcgccc
     4861 acgacgacat gagctacgtg ccgaaggaaa tggtcgagca ctggaccgcg cgcgacccga
     4921 tcgataacta cgagcgcaag ctgcgcgagg agggcttcga cacggatgag atccgtgagt
     4981 cggtgaaggg cgagctcgac gccgaaaccg agtgggcgct ggcacagccg atgcccgagc
     5041 cgcacaccgc tctcgagggc gtattcgcca ccgaggaccc ggtactcggc gacggcaacg
     5101 cgccctggtc gcgctggcag cgggaggcgg cgaatgcctg agatcacgta tctccaggcc
     5161 atctccgacg gcctgcgcga ggagatgcgc cacgacgagt ccgtgttctg cctcggcgag
     5221 gacatcggcg cgttcggcgg cgcgttcaag gtgaccgatg ggttcatcga ggagttcggc
     5281 gcagaccgcg tgtgggacac gccgctcgcc gagaacgcga tcatcggcac ggccgtcggc
     5341 gcagccgccg aggggctgcg gccggtttgc gagatgcagt tcgccgactt catctcgtgc
     5401 ggcttcgacc agctcgtgaa cgtcgccgcc aagatgcact accgccaggg gctcgccgtg
     5461 ccgatgacgg tgcggcttcc ctcgggcgga ggcttctccg gcggcccgtt ccattcgcag
     5521 aaccccgagg cctggttcct gcagtcgccc gggctgaagg tggtcgcgcc ctccacgccg
     5581 gaggatgcga agggcctgct ctccgcagcc atccgcgatc cgaaccccgt cgtctacctg
     5641 gagcacaagc acctctaccg ccgcgccaag ggcgaggtgg ccgagggccg tttcgaaatc
     5701 ccgctcggcg aggcgcgcgt cgcacgcgag ggcacggacc tcagcgtgat cacgtacggc
     5761 gcgatggtgc agctggcgct cgacgcgacc gaggacctcg acggcgcatc ggtcgaggtg
     5821 ctcgacctgc gctcgctcgt gccgctcgac accggcgcga tcctcgagtc ggtgcgcaag
     5881 acgagcaagg tcgtggtgct gcacgaggcc acgcgctcgt gcggagtcgg cgccgaggta
     5941 tcggcgctga tcgcacagga ggcgttcgag gatctagacg gcccggtggt ccgggtcacc
     6001 gcgcccgaca cgccggtgcc gttcagccct ccgctcgagc gggcggtgct gccgcagccg
     6061 gacgacgtga ggagggcgtg ccttgagctc ctcgcctact agcgcacaga cgaccgtcga
     6121 cgtggtcatg ccccagatgg gcgtgtccgt cgcggaggga acggttgccg aatggcgcaa
     6181 gcgcgtcggc gactgggtag aggcagacga gacgattctc gagatctcca ccgacaagat
     6241 cgagaccgag gtgccgtcgc cggcatccgg ccgcgtaacg gaggtactcg tcgacgtcgg
     6301 ctccacggtt gacgtgggga ccgtgctcgc gcgcatcgac accggcgcga agccgggcga
     6361 gccgcatgcg gacgaacacg cgccgcaggc tgggcccgcg gtggccgagc ccgccgccgc
     6421 ggaagcgggg ccgctgccgg agaaggccca cccgcccgag ccggctgagc acggcggcaa
     6481 cggtcacgga ccgccaatct cgcccgtggt ccggcgcatc gccgaggagc accagatcga
     6541 cctctcgcag atcgagggct ctgggcgccg cggccgcgtg accaagcgcg acgtgctggc
     6601 tttcctcgag cgccgcgatg atgcggcggc ggagaggccg cttcacatcg agtcgccgta
     6661 ccgcgacgag gagccagcac ccgtgcagga gccggcgccc gaacgcgagg ccgcgccggc
     6721 ggtcggcggc gagccgctgt cgctcatgcg tcagaagatc ggcgagcaca tgcgtcgctc
     6781 gctcgacacc gcggcgcact gcaccacgat cgtcgaggcg gacatgagcc gcatcgaggc
     6841 cgcgcgcaag agcggccgcg agaagctcac ctacctgccg ttcgtggcgc gtgcggtgat
     6901 cgcggcgctg cgcgagtacc cgatgctgaa cgccacgctg gagggcgacc ggctgaccgt
     6961 gcacgaggag gtgaacctcg gcgtagccgt gtcgctcggc gaggacgggc tgatcgtgcc
     7021 ggtggtgcac aacgcccacg agctctcgca cgagggcctt gcggcacgca tcaaggacct
     7081 cgcggagaga gcacgcgcca agcggctcga gccggacgag gtgcgcggcg gcacgttcac
     7141 gatcacgaac cccggcgcat acggcgcgct gctcgcgacg ccgatcatca accagccgca
     7201 ggtcgcgatc ctcgacctcg aggccgtcgt gaaacggccg gtcgtggtgg acggagatag
     7261 catcgccatc aggccgatga cctacctctg catgtcctgg gatcaccgcg cgctcgacgg
     7321 cgcgctgtcc gcgcagttcc tgtcggcggt tcgccggcac atcgaggagt ggagtggctg
     7381 agcccctctc cgagctctgg gtcgctcgga taccggggct cgtcccctac gcggagggcg
     7441 tcgcgctgca ggagcgcgtg cgcgccctcc gccaggagga ccgcattccc gacgcgctcc
     7501 tgctgctcga gcattcgccg gtgttcaccc gcgggcggcg aacggaggcg gcggacctgc
     7561 cgttcggcga cgactggtac cgcgagcgcg gcgtcgaggt tcacgacacc gaccgcggtg
     7621 ggcgggtcac ctaccacggg ccgggccagc tcgtgggcta cccgatcatg cgcatcgacg
     7681 acgtgctcga gtacgtgcgc accatggagc gagcgatgat cgccgcgctc tccgacgagg
     7741 ggatcgacgc cgggctacgc gaggggctca ccggcgtgtg ggtcgagaac cgcaagatcg
     7801 gctcgatcgg cgtgcacgtg tcgcgcggcg tgacgacgca cgggttcgcg gtgaacgtcg
     7861 agaacgacct cgagccgttc gagtgggtcg tggcctgcgg catcggcggc gtccagatga
     7921 cgtccgtccg gcaggagggc ggcgagggca tcgactgctt ccgcaagcga atggcgtggc
     7981 gcttcgccga ggc
//
LOCUS       DASVXS010000292        19968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_843227, whole genome shotgun sequence.
ACCESSION   DASVXS010000292 DASVXS010000000
VERSION     DASVXS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843227"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VF752_16350"
     CDS             <1..813
                     /locus_tag="VF752_16350"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8857167.1"
                     /translation="QEPITAHPLFGKPGVIVTPHLGASTAEAQDRAGVQTAEQILAAL
                     TGGEVTNAVNIPRMGAEDREVLGPFLPLCTRLGQIAIALGEASSVERVEVRYEGQLAD
                     YDTRLLNIAVLVGILEGHTEEAINYVNAPAIAEERGIALAEQKTRESEDFAELVAVTV
                     VSDGGAIEVAGTGFGPRNTPHLVGVYGQSFNIEMADHFAFFRYADQPGMIGRVGTIFG
                     EEGVNINSAAVGAEEGAEQAVMVVTTDAPVPQTLIDRIVTLDGFYTGRAVSL"
     gene            885..2348
                     /locus_tag="VF752_16355"
     CDS             885..2348
                     /locus_tag="VF752_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857168.1"
                     /translation="MARRTLLLVALGGCLLAIPAGVSWAPVVGTRVTHIVSPPDESPA
                     SNGPSDSDTFVEDRGGTRNDPIGGARPADHNVTFSQDNRKVKYVAYDSTATNLVSGDT
                     NGKRDVFVMTRKHPGTGSTDGTLSLGSVSSSGKQANADSMKPSLDGISTLGQRAAGVG
                     CLAFQSKATNLDSSDKSSDWDIYLRNLKSHKTVLASPGKSNAKDGVVDGWCERVSFEA
                     GGKVYVYDIERDVVTQVASGKDPDIQTNGQGVAFDQGGQVWYQALSHHGSKVAKDGGK
                     RLVSNSATNKSKGGNGVSSDPNLNNDGLYVVFESKATDLCIHRCRGISEDRNGHISDV
                     FRRTMGKSPTGGADEMEMVSYDGPTDTQGDLESDQVHITGHGEQAVFRSFANNLRDIK
                     FDHVAESGPFMHVYFWNFPRERLAGSISGESKCCRNGEFHHNDMSAAFSFSPQISNRG
                     TFIGFSSDEEGESGERNGRGTVDAFIRFMGLADEPGA"
     gene            complement(2360..3052)
                     /locus_tag="VF752_16360"
     CDS             complement(2360..3052)
                     /locus_tag="VF752_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857169.1"
                     /translation="MFLIGGGRDPEGVRVSHEPFVRACQGGPIAVVMLDEGDGVDEAR
                     WLDALLDAGASDLTPITIGEDQPPEPGALLGFAGVFVSGGLTPLYRELLVEQYPDWIP
                     EGIEDGAVYGGFSAGAAIAAERALVGGWQVERGDLQVQVCPEEASEDLELVTVLRGLA
                     LVPFTVDVHASQWGTLPRLLHAIADGAAPEGVAIDEHTCVEVAAGPQLRVHGLGTAYR
                     VTAAGAVSLLSP"
     gene            3281..4885
                     /locus_tag="VF752_16365"
     CDS             3281..4885
                     /locus_tag="VF752_16365"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX8857170.1"
                     /translation="MSDRPTKDMREEANRVRIFDTTLRDGEQSPGISLNRQEKVEIAQ
                     QLARLGVDVIEAGFPITSPGDFESVQAIAREVEGPVICALARTSSADIEAAGNAIKDA
                     ERGRIHTFIATSDIHIQHKLQTTREDVLGQARAAVAHARQYTDDVEFSPEDGSRSDIE
                     FMAEVIQAAVEEGATTINVPDTVGYTMPHEYAAMFEELYRLVPELRNAVVSVHCHNDL
                     GLAVANSLAGLEAGCRQVECAINGIGERAGNASLEEIVMLMRTRESSLGLWTGINTQE
                     IARTSRLVSRLTGYQVQPNKAIVGRNAFAHEAGIHQDGVLKERTTYEIMDATTIGLET
                     NSIVLGKHSGRHALRKALEELGFQVEGAALNTAFKRFKELADKKKKVTALDLEAIVSD
                     EMRESPTAYGLEWFDIEASSNRAPLARVGIRMPGGDVVEGSFTGDGPVDAFFSAINAA
                     TGHEARLKEYHVSAVTGGRDALGEVTVLLELGGELASGQGVATDILEASGRAYLRALS
                     NALATSPAVAEAEHHLREEAARTPTP"
     gene            4908..5930
                     /locus_tag="VF752_16370"
     CDS             4908..5930
                     /locus_tag="VF752_16370"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8857171.1"
                     /translation="MAAEGADFTWIDSERLIRFGVGVVDSAADLLEQRGFVRYALLTT
                     DRALASARHLAQGAESVLFVAPTPVPEAAASARDEVGGRPLVALGGGRVIDAAKAIAG
                     ADRLPVAAIPTTLSGAEMTQIHRMPAGVTEFNLVRPSLVIADPRLMASQPMPDIAASA
                     MNALAHGMEPLYTPFANPVAEMTALRGARLIAAALEGDGEPDRNDLALGSLLCAYALD
                     SAGYAVHHVVCQTIVRIALTPHAQTNAVMLPHTMAFMASRAPGAIGRLASALRAGRED
                     PALAPEEAVRLAARSGATRLSGIGFDEGRIDEVVAAALARPELANTPPSPPTGEELER
                     VIRHAL"
     gene            6020..6157
                     /locus_tag="VF752_16375"
     CDS             6020..6157
                     /locus_tag="VF752_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857172.1"
                     /translation="MLWLILGIILLVIAIAGGAIIHPILFALGVVAIVMFLMSRRGPA
                     Y"
     gene            6255..7832
                     /locus_tag="VF752_16380"
     CDS             6255..7832
                     /locus_tag="VF752_16380"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8857173.1"
                     /translation="MNGLMMDSPLLIRQIAEHAEAVFPQREIVSRTQDGTERSTYAQV
                     VGRARRLASALTELGIEPGDRVATFGWNSLRHLELYLAVPSMGAVLHTLNIRLFEEDL
                     RYIVGHAEDRVIFVDASLAGVMPKFDAVEYEVLMPDGAGERDGALAYEELVERGDPGF
                     EFPDFDENSAAAMCYTSGTTGRPKGIVYSHRSTVLHSMATGLSGANGLREDDSAMPVV
                     PMFHANAWGIPYTATLVGARQVFPGPGMTPDALAELIAAEKVTWAAGVPTIWQGVLQL
                     ENPDLSSLRELMAGGSAVPESLIRAYTERFGVPMVQGWGMTETSPVAALSRLPAGVEL
                     SEDEAYELRAAQGRPLPLVEWRIDEEAGGELQVRGPWVARSYYNDPASAEKFTDDGWL
                     RTGDVAEMRHGSFIKLVDRTKDLVKSGGEWISSVELENAVMAHPDVLEAAVIAVPDER
                     WGERPCACVVRCQGSDLDEEAVRSFLGERVAKWWVPERIEFVDEVPKTSVGKFDKKVL
                     RARYAEEAAGEPAAADG"
     gene            complement(7787..9001)
                     /locus_tag="VF752_16385"
     CDS             complement(7787..9001)
                     /locus_tag="VF752_16385"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8857174.1"
                     /translation="MSDGQLLTPDQLRERVERLAALERGSASRGERAASDLIEAELRG
                     LGLEVRRDVEVVHGGYWLPVGLATAAGVVAAAARRVPGVLLGSLAAAAVVDDIDAGPR
                     VLRRTLLAKRETANLSVEVGPPDAETTVLVVAHYDAPHSGLVFHPQLPRTVLRNFPAI
                     ASRAKTAPPTMWGAIAGPALSALGAALRSRRLRVAGGLLSAGYAVAMADIGLRGVVPG
                     ANDNASGVAAVLAVAHWLAAEPPPVRVILLFTGSEESLLEGMQRYGERHFARLDRERT
                     HVICLDTVGSPHLVALEAEGMVRMRHFPRRLLELLHECAGELGVYVQRGLSFRNTTDA
                     TVALKAGYPAVMIGSADEFMIPTDYHWPTDTADRVNYPTVADAARLVQAMIRRLGAQP
                     SAAAGSPAASSA"
     gene            complement(8998..9909)
                     /locus_tag="VF752_16390"
     CDS             complement(8998..9909)
                     /locus_tag="VF752_16390"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8857175.1"
                     /translation="MPETDHQMSTADRLYTLIGVFLPFAGFVTAIALLWNRGVGWSDL
                     AVLLLMYVLTGLGVTVGFHRLLTHRAFDSPRPVRYTFAVLGSMALQGSVIDWVSDHRK
                     HHAFADEEGDPHSPHGHGPGALGVLKGLWHSHMGWLFETQGTADHNRYARDLMEDRGM
                     RWIHRRFPLLAAATLALPASLGYLLTGTAAGALTGFIWGGLVRVFLLHHVTWSINSVC
                     HFAGRRRFATDDHSTNVFWLALPSFGESWHHNHHAFPRSARHGLRWWELDPSALVIVA
                     MKRVGLARSVVEISPSRQEERRAGAAI"
     gene            9977..11152
                     /locus_tag="VF752_16395"
     CDS             9977..11152
                     /locus_tag="VF752_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8857176.1"
                     /translation="MSDRTKSAPWRALDPSVADALRPALPRLVGDVIAAVKAAVPMYE
                     GVLDSNVQRGVHQALDGFLELVAGGDEASLPGRQVYVSFGRGESRNGRTLEALLTAYR
                     AGAQVAWRGLAEEGERAGLEPRAMYTLAEAVFAYIDELSSASAEGFAREQSALAQERQ
                     ERRRRMIELLLHDPRADPAQLEQAARAVPWDVPRRLAVVVFPEDAVRAVEPRLPGDAL
                     VAAGHGLATAVVPDPDAPKRRSDLERALRGHPAGIGSAVMPAAARRSAREAELAFGLA
                     QAGVEPAMVYARERLLDLMLAQDPELAAELARSALAPFDVLSPATRERLLGTLSAWLA
                     CHGSASAAAERLHVHVQTVRYRLDQVRDLLGSRLDDADGRLELQLALRISGTPSPQT"
     gene            complement(11124..11978)
                     /locus_tag="VF752_16400"
     CDS             complement(11124..11978)
                     /locus_tag="VF752_16400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8857177.1"
                     /translation="MAIQRDLHEENRRSWNEATRAHNSHKADQAGFLRGGGSTLFSDE
                     IELLGDISGKTLLHLQCNAGQDTLSLAGLGAKVTGVDISDEAIDFARKLSEDSGIPGT
                     FERADVLDWLPEAAAAGRRFDIVFVSYGAIYWLSDIKAWAEGIAGVLAPGGRLVMIEF
                     HPLGIFDDDWNIVYDYAFGGKHYRDEEGVNDYVSRSGEALAPGGYSEGETKFSNPHPA
                     HAFDWNLGDVVTALIEAGLRIELVREYPYSNGDKIYPRMRELPGNRFASPDGMPELPF
                     MFAVTASR"
     gene            12052..12639
                     /locus_tag="VF752_16405"
     CDS             12052..12639
                     /locus_tag="VF752_16405"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX8857178.1"
                     /translation="MSTATIEASAVGSRVRALREGMGLSLRDLAERSGVSAQMLSQVE
                     RGETSPTLAVAGRIASGLELSLSQLLRLDEGDGVTVVRRGERLRPAQRVAGHSLEVLT
                     PPLPGQRAEVSEHRLAPGAATGGPDDPPMHEPGSRETAIVLSGELRLVCDGRCHDLGA
                     GDAVTFDADLPHHFENPGQADAEFLAIVSAGLRRS"
     gene            12643..14025
                     /gene="leuC"
                     /locus_tag="VF752_16410"
     CDS             12643..14025
                     /gene="leuC"
                     /locus_tag="VF752_16410"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX8857179.1"
                     /translation="MAKTLLDKIWDAHEVAPGLLWIDLHLVHEVTSPQAFDGLRLAGR
                     GVRRPDRTLATADHNVPTDGSTRAEQIRDQLSRVQVETLERNCEEFGVPVYSIGSKHQ
                     GIVHVIGPELGVTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQVKP
                     KAMRILYTGELGFGVTPKDLILGTIGRMGTGGGVGHVIEYAGPVIERLSMEGRMTICN
                     MTIEGGGRAGMVAPDETTFEWLRGRPAAPDDLDAAIEEWRELHSDDGARFDRELEIDA
                     SEISPQVTWGTTPGMVTDVTSAVPEPQSDADDRALAYMGLEAGTPIEEIELDRVFIGS
                     CTNARIGDLRAAATMVEGRKVSPRVRAMVVPGSEQVRAQAEAEGLDEVFKAAGFDWRS
                     AGCSMCLGMNPDTLSPGERCASTSNRNFEGRQGRGGRTHLVSPEMAAAAAIEGHFVDI
                     RSWDREEVLA"
     gene            14026..14586
                     /gene="leuD"
                     /locus_tag="VF752_16415"
     CDS             14026..14586
                     /gene="leuD"
                     /locus_tag="VF752_16415"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX8857180.1"
                     /translation="MDPIRVVSGGVTVLDRADVDTDQIMPAQFLKRVERTGFGEFLFY
                     EWAKDPNWKLDKNRILVTGRNFGSGSSREHAPWGLQDYGFKAIVAPSFADIFFNNCTK
                     IGLLPVVLPEEDVRSLMQASEATVDLERQVVEWEGGSASFEIDPETRDRLLNGLDEIG
                     VTLNEEGAIAAYERERERTGPVTTAL"
     gene            14583..15356
                     /locus_tag="VF752_16420"
     CDS             14583..15356
                     /locus_tag="VF752_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8857181.1"
                     /translation="MSEHRPRDWDAKTYDRVSAPQFEWGRDVLDRLVLEGYETVLDAG
                     CGSGRITALLRERLPEGHVVAVDASPSMVEKAREALGDERVDYVVTDLVELELAEPVD
                     AVLSTATFHWILDQPRLFERLHAVMRPGGQLVAQCGGEGNVERFLSEVEDVMAREPFA
                     EQFEGWERPWYFGAPAATALALGDAGFTDVECWLEPRDAVLDDPRDFATTVCLSAHLD
                     HLPRELHDRFVDAVIERSGAPLVLDYVRLNINARRPADG"
     gene            15349..16371
                     /gene="leuB"
                     /locus_tag="VF752_16425"
     CDS             15349..16371
                     /gene="leuB"
                     /locus_tag="VF752_16425"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX8857182.1"
                     /translation="MADARILALPGDGIGPEIMAAARELLGRLGDFEIDERLVGGASI
                     DAHGTALTDDVLDACRHADAVLLAAVGGPKWDTTAPGAPRPEEGLLGLRKGLELFANL
                     RPVRPPSALLDSSPLRRDRIEGTDLLVVRELTGGIYFGDRGREDSRAFDTCVYSVEEI
                     ERIAEVAFRFARSKVSSVDKANILETSRLWRETVTSYARDKDVQLEHLLVDNAAMQLV
                     ARPSDFDVILTENMFGDILSDEAAMITGSIGILPSASVGAGGPGLFEPVHGSAPDIAG
                     SGRANPLAMFGSVAMMMRYGLGREELAAAIESAVDRALEDGLRTYDLGGDAGTEDATR
                     AVLANL"
     gene            16387..17313
                     /locus_tag="VF752_16430"
     CDS             16387..17313
                     /locus_tag="VF752_16430"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX8857183.1"
                     /translation="MQTADLIWLNGDFVAWEDAKVHVLTHGLHYGTGVFEGIRAYETE
                     RGTAIFRHLDHLDRLERSARFYYMDLPFSPAKMREATHELIARNGFRSCYIRPLVYRG
                     YGPMGLDPLTNPVEAMIAVWEWGSYLGEEGKRNGIRAKVSSWRRISPDSLYPHAKASG
                     QYLNSVLAKIEASKAGYEEAILLDHNGFVCEGSGENIFVVKDGVIYTPPQSTSILDGI
                     NRKSIITIAHDFGLEVVERDIARGELILADEVFMSGTAAELTPVREIDDYPLGPPGEI
                     TRRVQVKFEDALYGRAPEYAEWLDIVQVPSKA"
     gene            17427..19037
                     /gene="cimA"
                     /locus_tag="VF752_16435"
     CDS             17427..19037
                     /gene="cimA"
                     /locus_tag="VF752_16435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX8857184.1"
                     /translation="MAEAVVLYDTTLRDGMQGEGMSLSAEEKVRVAHALDSLGVPLIE
                     AGFPSSNPKEQALFDLLSAERFEHAEVAAFGMTRRRGVSAEDDPALRLLADCFAPACT
                     LVGKTWALHLEKVTRVDRDENLAMIADSVAFLRGSGKRVIYDAEHFFDAFREDPDYAL
                     RCLRTALEAGAENVTLCDTNGSSLPHQVAEAAARVVGEFGDGSQVGIHTHDDAGCGVA
                     NSLAAVDAGARLVQGTMNGYGERCGNANLTSILPALQLKMGYDCVAPELLARLTEVAH
                     LVDEICNVTPNANQPYVGKNAFAHKGGMHVAGVLADSRTFEHMEPAAVGNDRSVLVSE
                     LSGKGTVQARAEQSGLDLDDATALRVVERVKDLEHRGFQFEAADGSFDLLIRRETGDW
                     TPLFRLESWRAIVEKREDGKVMTEATIKIWVEGERYVRTAEGNGPVNALDKALRDAIG
                     EHYPHLRDVRLVNFKVRILDETKGTGAVTRVLLDASDGTDTWGSISVSENIIEASWEA
                     LVDSLEAGMLPGRTEHVRHSAPAEPAAP"
     gene            19034..>19968
                     /locus_tag="VF752_16440"
     CDS             19034..>19968
                     /locus_tag="VF752_16440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8857185.1"
                     /translation="MSQEPIPLAKPLIGEREEQLVLDTLRSGRLSLGPRLGELEDAFA
                     QRLGVPSVSAVSSGTAGLHLAVRAAGIEAGDEVITTPFSFVASANCVLYENARPVFCD
                     IDPRTLNIDGEAAAAAVTERTTGLLPVHIFGYPADLPALERIGAQRGLWIVEDACEAL
                     GARHADGKPVGARGHQAVFAFYANKQITTGEGGMVVSEHKEKIESERNQGRAPDMGWL
                     DHDRLGFNYRLTDIACALGIAQLERLDDLLDGRAAAAALYSGALAEIEGLELPCPDSG
                     GDVRGWFVFVVQLPEGRDRDATILALREQGVQSKP"
BASE COUNT         3180 a         6880 c         7054 g         2854 t
ORIGIN      
        1 caggagccga tcaccgcgca cccgctgttc ggcaagccgg gagtgatcgt caccccgcac
       61 ctcggtgcct cgacagcgga ggcgcaggat cgcgccggcg tgcaaacagc cgagcagatc
      121 ctcgcggcgc tcactggcgg cgaggtcacg aacgcggtga acatcccgcg catgggcgcc
      181 gaggaccgcg aggtgctcgg tccgttcctg ccgctgtgca cgcgcctcgg gcagattgcg
      241 atagcgctcg gcgaggcctc gtcggtggag cgcgtcgaag tgcgctacga aggccagctc
      301 gccgactacg acactcgcct gctcaacatc gccgtgctcg ttggcatcct cgagggccac
      361 acggaggagg cgatcaacta cgtgaacgcg ccggcgatcg ccgaggagcg tggcatcgcc
      421 ctggccgagc agaagacgcg ggagtccgag gacttcgccg agctcgtcgc cgtcaccgtc
      481 gtctcggacg gcggcgcgat cgaggtggcg ggtactggct tcggcccacg caacacgccg
      541 cacctcgtgg gcgtctacgg ccagagcttc aacatcgaga tggccgacca cttcgcgttt
      601 ttccgctacg cggaccagcc gggcatgatc ggccgagtgg gcacgatctt cggcgaggag
      661 ggcgtgaaca tcaactcggc cgcggtcggg gccgaggagg gcgccgagca ggcggtgatg
      721 gtggtcacca cggacgcccc ggtgccgcag accctgatcg accgcatcgt gacgctcgac
      781 ggcttttaca caggccgcgc tgtttcactc tagatcgctt gcgcaaccgg cggcgccggg
      841 gttagcgtga aggcggaccc gcacccgtcg ccggaggact cgcaatggcc cgccgtaccc
      901 tcctgctggt cgcccttggt ggctgcctgc tcgccatccc ggccggtgtg agctgggcgc
      961 cggtggtcgg cacgcgcgtt acgcacatcg tctcgccgcc ggacgagtct ccagcctcta
     1021 acggacccag cgactccgac accttcgtcg aggaccgcgg cggcacgcga aacgacccga
     1081 tcggcggcgc ccggccggcg gaccacaacg tcaccttcag ccaggacaac cgcaaggtga
     1141 agtacgtggc ctacgactcg acggccacga acctggtgtc cggcgacacc aacggcaagc
     1201 gcgacgtgtt cgtcatgacg cgcaagcatc ccggcacggg ctcgaccgac ggcacgctca
     1261 gccttggaag cgtgtcgagc agcggcaagc aggcgaacgc cgacagcatg aagccctcgc
     1321 tcgacggcat cagcacgctc ggccagaggg cggcgggcgt cggctgcctc gcgttccagt
     1381 ccaaggcgac gaacctcgac agctccgaca agagctcgga ttgggacatc tacctgcgca
     1441 acctgaagag ccacaaaacc gttctggcga gtccgggcaa gtcgaacgcg aaggacggcg
     1501 tggtcgacgg ctggtgcgag cgcgtgagct tcgaggcggg cggcaaggtc tacgtctacg
     1561 acatcgagcg cgacgtcgtg acccaggtcg ccagcggcaa ggaccccgac atccagacga
     1621 acggccaggg cgtggctttc gaccagggcg gacaagtctg gtaccaggct ctgtcgcacc
     1681 acggcagcaa ggttgcgaag gacggcggca agcgcctcgt gagcaactcg gccacgaaca
     1741 agtcgaaggg cggcaacggc gtcagcagcg acccgaacct gaacaacgac ggcctctacg
     1801 tggtgttcga gtcgaaggcc acagatctct gcatccaccg ctgccgcggc atctcagagg
     1861 accgtaacgg acacatttcg gacgtcttcc gccgcacgat gggcaagagc ccgacgggcg
     1921 gcgccgacga gatggagatg gtcagctacg acgggccgac cgacacgcag ggtgacctcg
     1981 agtccgacca ggtgcacatc accggtcacg gcgagcaggc ggtgttccgc tcgttcgcga
     2041 acaacctgcg cgacatcaag ttcgaccacg tcgcggagag cggtccgttc atgcacgtgt
     2101 acttctggaa tttcccgcgc gagcggctgg ccggctcgat ctcaggcgag agcaagtgct
     2161 gccgcaacgg cgagttccac cacaacgaca tgagcgcggc cttcagcttc agcccccaga
     2221 tctcgaaccg aggcacgttc atcgggttct cgagcgacga ggagggcgag tccggcgagc
     2281 ggaacggccg cggcaccgtg gacgccttca tccgcttcat ggggctcgcc gacgagccgg
     2341 gcgcgtagcg gcgcgcagtt cagggcgaga ggaggctcac cgcgccggcc gcagtcacgc
     2401 ggtacgccgt tccaaggccg tgcacgcgca gctgagggcc agccgcgacc tccacgcatg
     2461 tgtgctcgtc gatcgcgacg ccctcgggcg cggccccgtc cgcgatcgcg tggagcagcc
     2521 gcggcagcgt tccccactgc gacgcatgca catcgacggt gaacggcaca agggccaagc
     2581 cacgcaacac cgtgacgagc tccaagtcct cgctcgcctc ctccggacag acctgcacct
     2641 ggaggtcgcc gcgctcgacc tgccagccgc ccacgagcgc gcgctcggcg gcgatcgccg
     2701 ccccggccga gaagccgccg tagacggcgc cgtcctcgat cccctcggga atccagtcgg
     2761 ggtactgctc cacaagcagc tcgcggtaga gcggagtgag cccgccggac acgaacacac
     2821 ccgcgaagcc gagtagcgct ccgggctcgg gcggctggtc ctcgccgatc gtgatcggcg
     2881 tgaggtccga cgccccggcg tcgagcagag catcgagcca gcgcgcctcg tcaacgccgt
     2941 cgccctcgtc gagcatcacg acagcgatcg gcccaccctg gcaagcccgc acgaacggtt
     3001 cgtgcgacac gcggactccc tccgggtcgc ggccgccgcc gatcaggaac acgcccggca
     3061 cgctaccggg cgtggacctg ggccgcgtgc gaaggcgcgt gaatcacgca cgccctcggc
     3121 agcgagttcc gcaccgtgta ggcttgccgg accgtgcagt tcccgcttga cctgcgactt
     3181 ctcctccttc tcccccttcg gggggaataa cgcgctggcg gccgcagaag ccgcgaaccg
     3241 acaccgagga gaaagaaggt caggacacga aaggaaccgc atgagcgacc gaccaacaaa
     3301 ggacatgagg gaagaggcga accgagtacg catcttcgac acgaccctgc gcgacggcga
     3361 gcagtcgccc ggcatctcgc tgaaccgcca ggagaaggtc gagatcgccc agcagctcgc
     3421 gcgcctcggc gtcgacgtca tcgaggccgg cttcccgatc acctcgcccg gagacttcga
     3481 gtcggtccag gccatcgccc gcgaggtcga gggccccgtg atctgcgctc tcgcgcgcac
     3541 ctcgtccgcg gacatcgagg ccgcgggcaa tgcgatcaag gacgccgagc gcggccgcat
     3601 ccacaccttc atcgcaacca gcgacatcca catccagcac aagcttcaga ccacgcgcga
     3661 ggacgtgctc ggccaggcgc gtgccgcggt ggcacacgcg cgacagtaca ccgacgacgt
     3721 cgagttctcg cccgaggacg gctcccgctc ggacatcgag ttcatggccg aggtgatcca
     3781 ggctgccgtt gaggagggcg cgaccacgat caacgtgccc gacacggtcg gctacacgat
     3841 gccgcacgag tacgccgcca tgttcgagga gctctaccgg ctcgtgcccg agttgcgcaa
     3901 cgcggtcgta tccgtgcact gccacaacga cctcggcctc gccgtggcca actcgctcgc
     3961 cggtctcgag gccggctgtc gccaggtgga gtgtgcgatc aacggcatcg gcgagcgcgc
     4021 cggcaacgcg tcgctcgagg agatcgtgat gctgatgcgc acgcgcgagt cgagcctcgg
     4081 tctctggacc gggatcaata cgcaggagat cgcgcggacc agccgccttg tctcgcggct
     4141 caccggttat caggtgcagc cgaacaaggc gatcgtcggc cgcaatgcgt tcgcccacga
     4201 ggccggcatc caccaggacg gcgtgctcaa ggagcgcacc acctacgaga tcatggacgc
     4261 cactacgatc ggcctcgaga ccaactcgat cgtgcttggc aagcactctg gccgccacgc
     4321 gctgcgcaag gctctcgagg agctcggctt ccaggtggaa ggcgcggcgc tgaacacggc
     4381 gttcaagcgc ttcaaggagc tcgctgacaa gaagaagaag gtgaccgcgc tcgacctcga
     4441 ggcgatcgtc tccgacgaga tgcgcgagtc gccgaccgcg tacggcctcg agtggttcga
     4501 catcgaggcg tcatcgaacc gggcgccgct cgcgcgcgtg ggcatccgca tgcccggcgg
     4561 cgacgtggtg gaaggcagct tcaccggcga cggcccggtg gacgccttct tcagcgccat
     4621 caacgccgcc accggccacg aggcgcggct gaaggagtac cacgtgagcg ccgtcaccgg
     4681 cgggcgtgac gcactcggcg aggtcacggt cctgctcgag ctcggcggcg agctggcctc
     4741 gggccagggc gtcgccaccg acatcctcga ggcgtcgggt cgcgcctacc tacgcgcgct
     4801 gtcgaacgcg ctcgccacgt cgcccgcggt ggccgaggcc gagcaccacc tccgcgaaga
     4861 ggccgcccgg acgcccactc cgtaacgccc gttagtctgc cgtctcggtg gctgccgagg
     4921 gggccgactt cacctggatc gacagcgagc gcctgatccg cttcggcgtg ggtgtcgtcg
     4981 actcggcggc ggatctgctc gagcagcgcg gcttcgtccg ctacgcgctt ctcacaaccg
     5041 atcgggcgct ggcgagcgcg cggcacctgg ctcaaggagc ggagagcgtg ctgttcgtgg
     5101 cgccgacgcc cgtgcccgag gccgcggcgt cggcgcgcga cgaggtgggc ggccggccgc
     5161 tcgtggcgct cggcggtggc cgagtaatcg acgccgccaa ggcgatcgcc ggtgcggacc
     5221 gcctccctgt ggccgcgatc ccgacaacgc tgtcgggcgc cgagatgaca cagattcacc
     5281 gcatgccggc cggagtaact gagttcaacc tcgtacgccc atcgctcgtg atcgccgatc
     5341 cgcggctgat ggcctcacag ccgatgcctg acatcgccgc cagcgcgatg aacgcgctcg
     5401 cgcacggcat ggagccgttg tacacgccct tcgccaatcc ggtggccgag atgaccgcgc
     5461 tgcgcggcgc tcgcctcatc gcagcggcgc tcgaaggcga cggcgagcca gatcggaacg
     5521 acctcgccct cggatcgctt ctgtgcgcct atgcgctcga ctccgcggga tacgcggtgc
     5581 accacgtggt gtgccagacg atcgtgcgca tcgccctgac cccgcacgcg cagacaaacg
     5641 ccgtgatgct gccccacacg atggcgttca tggcgagccg cgcgccaggg gcgatcggcc
     5701 ggctcgcatc ggcgctccgc gctggacgcg aagacccggc gcttgcgccg gaggaggcgg
     5761 ttcgcctcgc cgcgcgctcg ggagcgacgc gtctgagcgg catcggcttc gacgaaggcc
     5821 gcatcgacga ggtggtcgcg gccgcgctcg cgcgtccgga gctcgcgaac acgccgccgt
     5881 cgccgcccac cggcgaggag ctcgagcgtg ttatccggca cgccctttga aggcatgccg
     5941 cgagcacaga cggggtaccc ctcaataggt aaccgatgcg gccgccgcca cggcggtggc
     6001 gaagggagga agtgcctgaa tgctctggct gatccttgga atcatcctgc tcgtgatcgc
     6061 gatcgcgggc ggagcaatca tccatccgat cctgtttgcg cttggagtcg tcgcgatcgt
     6121 gatgttcctc atgtccaggc gcggcccggc gtactgacca gtctcgagac tcgcacggac
     6181 gcgaaggagg cggagcggga cgttggttcg ccgtcccgct cgcccatcgg gctcgcacgg
     6241 gtaggctgcc gcccatgaac ggcctgatga tggactcacc gctgctgatc cggcagatcg
     6301 ccgagcacgc cgaagccgtc tttccgcagc gcgagatagt cagccgcacg caggacggca
     6361 ccgagcgctc cacctatgcg caggtggtgg ggcgggcgcg ccgtctggcg tcggcgctca
     6421 ccgagctggg gatcgagccg ggggaccgcg tggccacctt cggctggaac tcgcttcggc
     6481 acctcgagct gtacctcgct gtcccaagca tgggcgcggt gctgcacacg ctcaatatca
     6541 ggctgttcga ggaggacctg cgctacatcg tcgggcatgc cgaggaccgc gtgattttcg
     6601 tcgacgcctc gctggcgggc gtcatgccga agttcgacgc cgtggagtac gaggtgctga
     6661 tgccggacgg cgccggtgag cgcgacggcg cgctcgccta cgaagagctt gtcgagcgcg
     6721 gcgaccccgg cttcgagttc cccgacttcg acgagaacag cgccgccgcg atgtgttaca
     6781 cgagcggcac caccggccgg ccgaagggca tcgtctactc gcaccgctcc acggtcctcc
     6841 actcgatggc gaccggcctc tcgggcgcga acggcctgcg cgaggacgac tcggccatgc
     6901 cggtcgtgcc gatgttccac gcgaatgcct ggggcatccc gtacacggca acgctggtgg
     6961 gcgcgcgaca agtgttcccg ggacccggga tgacgccgga cgcgctcgcc gagctgatcg
     7021 ctgccgagaa ggtcacctgg gccgcgggcg tgccgacgat ctggcagggc gtgctgcagc
     7081 tcgagaaccc cgacctctcg agcctgcgtg agctgatggc gggcggctcc gccgtcccgg
     7141 agtcgctcat tcgcgcctat accgagcgct tcggcgtgcc gatggtgcag ggctggggta
     7201 tgaccgagac gagcccggtc gccgctctgt cgcggctgcc cgccggggtg gagctctccg
     7261 aggacgaggc ctacgagctc cgcgccgcgc agggccgccc gctgccgctg gtggagtggc
     7321 ggatcgacga ggaggccggc ggcgagctgc aggtgcgcgg tccctgggtg gcgcgcagct
     7381 actacaacga cccggcctca gcggagaagt tcaccgacga cggctggctc cgcaccggcg
     7441 acgtcgcgga gatgcgccac ggctcgttca tcaagctggt ggaccgcacg aaggacctcg
     7501 tgaagtcggg tggcgagtgg atctcgtccg tggagctgga gaacgcggtg atggcgcacc
     7561 ccgacgtgct cgaggctgcg gtgatcgccg tgcccgacga gcgctggggc gaacgccctt
     7621 gcgcgtgcgt ggtgcgctgc cagggctcgg atctcgacga ggaggcggtg cggtcgttcc
     7681 tcggcgagcg cgtcgcgaag tggtgggtgc ccgagcggat cgagttcgtc gacgaggtgc
     7741 cgaagacctc ggtcggcaag ttcgacaaga aggtgctccg cgcccgctac gcggaagagg
     7801 cggccggcga accggcagcc gctgacggct gagcgccgag ccggcggatc atcgcctgaa
     7861 ccagccgagc cgcgtccgcg accgtggggt agttcacgcg gtcggcggtg tcggtcggcc
     7921 agtggtagtc ggtcgggatc atgaactcat ccgccgagcc gatcatcacc gccggatagc
     7981 cggccttcag cgccacggtc gcgtctgtgg tgtttcggaa gctgagcccg cgctgcacgt
     8041 agacgccgag ctcgcccgcg cactcgtgga ggagctcgag caagcgccgc gggaagtggc
     8101 gcatccggac cattccctcc gcctcgaggg cgacgaggtg gggagagccg accgtgtcga
     8161 ggcagatgac gtgcgtacgc tcgcggtcga gcctcgcgaa gtggcgctcg ccgtagcgct
     8221 gcatgccctc gagcagcgac tcctcggagc ccgtgaacag gaggatcacg cggacgggcg
     8281 gcggctcggc cgccagccag tgggcgacag cgagcactgc ggcgacgccg ctggcgttgt
     8341 cgttggcgcc cgggacgacg cccctgaggc cgatgtcggc catcgcgacc gcatagcccg
     8401 ccgaaagcag cccgcccgcc acgcgaagcc gcctgcttcg gagcgcggcg ccaagcgcgc
     8461 tcagcgcggg cccggcaatc gcgccccaca tggtcggggg cgcggttttc gccctgctgg
     8521 cgatcgccgg aaaatttcgc agcacagtcc gtggcagctg tggatggaac acgagtcccg
     8581 agtggggggc gtcgtagtgg gccacgacca gcacggtcgt ctcggcgtcg ggcgggccga
     8641 cctccacgct gaggttggcg gtctcgcgct tcgcaagcag cgtccggcgg agcacgcgcg
     8701 gcccggcgtc gatgtcgtcg accaccgccg ccgctgcgag cgagccgagc agcacgccgg
     8761 ggacgcggcg cgcggcggcg gcgacgacgc cggccgcggt ggcgaggccg acgggcagcc
     8821 agtagccgcc gtggacaacc tcgacgtcac gccgcacttc gagccccagg ccgcggagct
     8881 cggcctcgat cagatccgac gccgcgcgtt cgccccggct ggccgagccg cgctcgagcg
     8941 ccgcgagacg ttcgacgcgc tcgcgtagct ggtccggtgt cagcagctgc ccgtcgctca
     9001 aatggctgct ccggcgcgcc gctcttcctg ccggctcggg ctgatctcga cgacgctgcg
     9061 cgccaggccg actcgtttca tcgcgacgat caccagcgcg gagggatcga gctcccacca
     9121 gcgcagcccg tgacgcgccg agcgcgggaa ggcgtggtgg ttgtggtgcc aggactcgcc
     9181 gaacgagggg agggccagcc agaagacgtt ggtggaatgg tcatcggtgg cgaaccgccg
     9241 gcgtccggcg aagtggcaga ccgagttgat gctccacgtg acgtggtgta gcaggaagac
     9301 gcgcaccagg ccgccccaga tgaagccggt gagggctcct gctgccgtcc cggtgagcag
     9361 gtagccgagc gacgcgggga gcgcgagcgt cgcggccgcc agcaggggga agcgccggtg
     9421 gatccagcgc atgccgcggt cttccatcag gtcgcgagcg taccggttgt ggtcggcggt
     9481 gccctgcgtc tcgaacagcc agcccatgtg cgagtgccag agacccttca gcaccccgag
     9541 cgccccgggg ccgtggccgt gaggcgagtg cgggtcgccc tcctcgtcgg cgaaggcgtg
     9601 atgctttcga tggtccgata cccagtcgat caccgagccc tggagtgcca tcgacccgag
     9661 cacagcgaac gtgtagcgca caggcctcgg gctgtcgaat gcgcggtggg tgaggaggcg
     9721 gtggaagccg accgtgactc ccagcccagt gagcacgtac atcaacagca gcacggctag
     9781 gtcgctccag ccgacgcccc tgttccagag gagcgcgatc gccgtcacga agccggcgaa
     9841 gggcaggaac actccgatca gcgtgtagag ccggtcggcc gtcgacatct gatggtcggt
     9901 ctctggcatt ctctgagcgt atggatggct ttgggcgatc ctctacggcc aaaaggcaca
     9961 gtcgatgacg caatctttga gcgatcgcac aaaatcggca ccctggcgcg ctctcgatcc
    10021 gtcggtcgcc gacgcgctcc gcccagcgct cccgcgcctc gtgggcgatg tgatcgccgc
    10081 agtgaaggcg gcggtgccca tgtacgaggg cgtgctcgac tcgaacgtgc agcgcggcgt
    10141 gcaccaggcg ctcgacggct ttctcgagct ggtcgcgggc ggcgacgagg cgagcctgcc
    10201 gggacgccag gtgtacgtga gcttcgggcg cggcgagtcc cgcaacgggc gcacgctgga
    10261 ggcgcttctc accgcctacc gggccggggc gcaggtggcc tggcgcggtt tggccgagga
    10321 gggcgagcgc gccggcctcg agccgcgcgc gatgtacacg ctcgccgagg cggtattcgc
    10381 ctacatcgac gagctgtcct ccgcctcggc cgagggcttc gcgcgcgagc agtcggcgct
    10441 cgcgcaggag cggcaggagc ggcggcggcg catgatcgag ctgctcctgc acgatccgcg
    10501 cgcggatcca gcgcagctag agcaggcggc gcgggcagtg ccctgggatg tgccgcgccg
    10561 ccttgccgtt gtggtcttcc ccgaggatgc ggttcgtgcg gtcgagccgc gtctccccgg
    10621 cgacgcgctc gtggccgccg gccacggtct cgccactgcg gtggtacccg acccggacgc
    10681 tcccaagcgc cgttcggatc tcgagcgcgc gcttcgcggc caccccgcgg gaatagggtc
    10741 ggccgtcatg ccggcggcgg cgcgccgttc ggcgcgcgag gcggagctcg cgttcgggct
    10801 cgcgcaagca ggcgtcgagc cggcgatggt gtacgcccgc gagcgcctgc tcgatctgat
    10861 gctcgcgcag gatcccgagc tcgcagccga gctggcccgc tcagcgcttg cgccctttga
    10921 cgtgctatcg cccgccacgc gcgagcggct gctcggcacg ttgagcgcgt ggcttgcctg
    10981 ccacggtagc gcgagcgccg cagccgagcg gttgcacgtg cacgtgcaga ccgtgcgcta
    11041 ccggctcgac caggttcgcg acctgctcgg aagcaggctc gacgacgccg acggccgcct
    11101 cgagctccag ctggcgctcc ggatcagcgg gacgccgtca ccgcaaacat gaacggcagt
    11161 tccggcatgc cgtccggcga ggcgaagcgg ttgcccggga gctcgcgcat gcgcgggtag
    11221 atcttgtcgc cgttcgagta ggggtattcg cgcaccagct cgattcgaag gcccgcctcg
    11281 atcagcgcgg tcaccacgtc gccgaggttc cagtcgaagg cgtgcgcggg atgggggttg
    11341 gagaacttgg tctcaccctc cgagtaacca ccgggcgcga gcgcttcgcc cgagcggctc
    11401 acgtagtcgt tcacgccctc ctcgtcgcgg tagtgctttc cgccgaaggc gtagtcgtag
    11461 acgatgttcc agtcgtcgtc gaagatcccg agcgggtgga actcgatcat cacgaggcgg
    11521 ccgccgggcg ccagcacgcc cgcgatccct tcggcccatg ccttgatgtc gctcagccag
    11581 tagatcgctc cgtaggagac gaacacgatg tcgaagcggc gtccggcggc ggctgcctcg
    11641 gggagccagt cgagcacgtc ggcgcgctcg aacgtgcccg gtatcccaga gtcctcggac
    11701 agcttgcgcg cgaagtcgat cgcctcgtcg ctgatgtcga cgccggtcac cttggcgcca
    11761 agcccggcga gcgagagcgt gtcctgcccg gcgttgcact ggagatgcag cagcgtcttg
    11821 cccgagatgt cgccgagcag ctcgatctcg tcggagaaga gcgtgctgcc gcccccgcgc
    11881 aggaacccgg cctggtcagc cttgtgactg ttgtgcgctc tcgtggcctc gttccacgag
    11941 cgccggtttt cctcgtgcag gtctctctgg atcgccattg cggccgtcaa ttcaagccga
    12001 aggtgtcaac ctgacgacta acggacagat gtccgtgata acgtacaaac cgtgagtact
    12061 gccacgatcg aggcgagcgc agtcggcagc cgcgtgcgcg cgctccgcga gggcatgggc
    12121 ctatcgctgc gagacctggc ggagcgaagc ggcgtgtcgg cgcagatgct ctcgcaggtc
    12181 gagcgcggcg agacgagccc cacgctcgcc gtcgcgggcc gcatcgcgag cggcctcgag
    12241 ctctcgctct cacagctctt gcggctcgac gagggcgacg gcgtgaccgt cgttcggcgt
    12301 ggcgagcggc ttcgcccggc ccaaagggtg gcgggccaca gcctcgaggt gctcacgccg
    12361 ccgctgcctg gccagcgcgc cgaagtatcc gagcaccggc ttgccccggg agccgccacg
    12421 ggtggcccgg acgacccgcc gatgcacgag cccggcagcc gcgagaccgc gatcgtgctg
    12481 agcggcgagc tcaggctcgt ctgcgacggc aggtgccatg atctcggcgc cggcgatgcg
    12541 gtcaccttcg acgcggatct tcctcaccac ttcgagaacc ccggccaggc cgatgcggag
    12601 tttcttgcga tcgtctccgc gggcctgagg agaagctgaa cgatggcgaa gacgctgctc
    12661 gacaagatct gggacgcgca cgaggtggcg cccggcctgc tgtggatcga cctacacctc
    12721 gtgcacgagg tgacttctcc tcaggcgttc gacggcctgc gcctcgccgg ccggggcgtg
    12781 cgccgcccgg accggacgct cgccacggcc gaccacaacg tgccgaccga cggctccacc
    12841 cgcgccgagc agatccgcga ccagctctcg cgcgtgcagg tggagacact cgagcgcaac
    12901 tgcgaggagt tcggcgtgcc ggtctactcg atcggctcga agcaccaggg catcgtgcac
    12961 gtgatcggtc ccgagctcgg cgtcacgcag ccgggcatga cgatcgtgtg cggcgactct
    13021 cacaccgcca cacacggcgc cttcggcgcg cttgcgttcg gcatcggcac gagcgaggtg
    13081 gagcacgtgc tcgcaaccca gacgcttcgc caggtgaagc cgaaggcgat gcgcatcctc
    13141 tatacgggcg agctcggctt cggcgtcact cccaaggacc tgatcctcgg cacgatcggg
    13201 cgcatgggca cgggcggcgg cgtgggccac gtgatcgagt acgccggccc ggtcatcgag
    13261 cgcctctcga tggaaggccg catgaccatc tgcaacatga cgatcgaggg cggcggccgc
    13321 gccgggatgg tcgcgcccga cgagaccacc ttcgagtggc tgcgcgggcg cccggctgcg
    13381 cccgacgacc tcgacgcggc gatcgaggag tggcgcgagc ttcactcgga cgacggcgcc
    13441 cgcttcgaca gggagctcga gattgatgcc tccgagatct cgccgcaggt cacgtggggc
    13501 acgacgcccg ggatggtgac cgacgtgacg tctgcggtgc ccgagccgca gagcgacgcc
    13561 gacgaccgtg cgctcgcgta catgggtctc gaggccggga cgccgatcga ggagatcgag
    13621 ctcgatcgcg tgttcatcgg ctcgtgcacc aacgcgcgca tcggcgacct gcgcgccgcc
    13681 gcgacgatgg tcgagggccg caaggtgagc ccgcgcgtgc gagccatggt cgtgcccggc
    13741 tccgagcagg tgcgcgcgca ggccgaggcg gagggtctcg acgaggtgtt caaggccgcc
    13801 ggtttcgact ggcgctcggc gggttgctcg atgtgcctcg gcatgaaccc ggacacgctg
    13861 tcgccgggcg agcgctgcgc ctcgacctca aaccgcaact tcgaaggccg ccagggccgc
    13921 ggtggccgca cgcacctggt cagcccggaa atggcggccg ctgccgcgat cgagggccac
    13981 ttcgtggaca tccgatcctg ggaccgtgag gaggtgctcg cgtagatgga tccgatccga
    14041 gtcgtcagcg gcggggtgac ggtgctcgac cgcgccgacg tcgacaccga ccagatcatg
    14101 cccgcgcagt tcctcaagcg cgtcgagcgc accggcttcg gcgagttcct cttctacgag
    14161 tgggcgaagg acccgaactg gaagctcgac aagaaccgga tcctcgtgac cggtcggaac
    14221 ttcggcagcg gctcgtcgcg cgagcacgcc ccctggggcc tccaggacta cggcttcaag
    14281 gcgatcgtcg cgccgagttt tgcggacatc ttcttcaaca actgcacgaa gatcggcctg
    14341 ctgcctgtgg tgctgcctga agaggacgtg cgctcgctga tgcaggcaag cgaggcgacc
    14401 gtggacctcg agcgccaggt cgtggagtgg gagggcggaa gcgcgtcctt cgagatcgac
    14461 ccggagacgc gcgaccggct gctgaacggt ctcgacgaga tcggcgtgac gctcaacgag
    14521 gagggggcga tcgcggccta cgagcgcgag cgcgagcgaa ccggcccggt gaccacggcg
    14581 ctgtgagcga gcaccgcccc cgcgactggg acgcgaagac gtacgaccga gtgtcggcgc
    14641 cccagttcga gtggggccgc gacgtgctcg accgcctggt tctcgagggc tacgagaccg
    14701 tcctcgacgc cggctgcggc agcgggcgca tcaccgcgct gctgcgcgag cggctgccgg
    14761 agggccatgt cgtggcggtg gatgcctcgc cgtcgatggt ggagaaggcc cgcgaggcgc
    14821 tcggcgacga gcgcgtggac tacgtggtga ccgatctcgt cgagctggag ctcgccgagc
    14881 ccgtcgacgc ggtgctgtcc acggcgacct tccactggat cctggaccag ccacggctgt
    14941 tcgagcgcct ccacgcggtg atgcgccccg gcggacagct cgtcgcgcag tgcgggggag
    15001 agggcaacgt cgagcgtttc ctctcggagg tggaggacgt gatggccaga gagccattcg
    15061 ccgaacagtt cgaggggtgg gaacgaccct ggtatttcgg cgcgccggcg gccacagcgc
    15121 tcgcgctcgg cgatgcgggc ttcaccgacg tcgaatgctg gctcgagccg cgcgacgccg
    15181 ttctcgacga cccccgcgac ttcgccacca cggtgtgcct gtcggcccat ctggaccacc
    15241 taccgcggga gctgcacgat cgattcgtgg acgccgtgat cgagcgctcc ggcgcaccgc
    15301 tggtgctcga ctacgtgcgc ctgaacatca acgcccggag gcccgccgat ggctgacgcc
    15361 cggatccttg cgctgcccgg ggacggcatc ggccccgaga tcatggccgc cgcccgcgag
    15421 ctgctcggcc ggctcggcga cttcgagatc gacgagcggc tggtgggcgg cgcttcgatc
    15481 gacgcgcacg gaaccgcgct caccgacgac gtgctcgacg cctgccgcca cgccgacgcc
    15541 gtgctgctcg ccgcggtggg cgggcccaag tgggacacca ccgcccccgg cgcgccccgt
    15601 cccgaggagg ggctgctcgg cctgcgcaag gggctcgagc tgttcgcgaa cctgcgcccg
    15661 gtgcggccgc cgagcgcgct gctcgactcg agcccgttgc ggcgcgaccg catcgagggc
    15721 actgacctgc tcgtggtgcg cgagctcacc ggcggcatct acttcggcga ccgcggtcgc
    15781 gaggactcgc gcgccttcga cacctgcgtc tacagcgtgg aagagatcga acggatcgcc
    15841 gaggtggcgt tccgcttcgc acgcagcaag gtctcgagcg tggacaaggc gaacatcctc
    15901 gagacctcgc gcctgtggcg agaaacggtc acgagctacg cgcgcgacaa ggacgtgcag
    15961 ctcgagcacc tgctcgtgga caacgcggcg atgcagctcg tcgcgcggcc gtccgacttc
    16021 gacgtgatcc tcaccgagaa catgttcgga gacatcctga gcgacgaggc ggcgatgatc
    16081 accgggtcga tcgggatact gccgagcgcg tcggtgggcg ccggggggcc cggcctgttc
    16141 gagcctgttc acggctcggc gcccgacatc gccgggagcg gtcgcgccaa tccgctcgcg
    16201 atgttcggct cggtggcgat gatgatgcgc tacggcctcg gtcgcgagga gctggcggcg
    16261 gccatagaat cagcggtgga tcgggcgctc gaggacggtt tgaggaccta tgacctcggc
    16321 ggcgacgccg gtacggagga cgccacgagg gccgtcctcg cgaacctcta gacaaggagg
    16381 aagtacgtgc aaaccgcgga cctcatctgg ctgaacggag acttcgtcgc ctgggaggac
    16441 gcaaaggtgc acgtactcac gcacggcctg cactacggca ccggcgtgtt cgagggcatc
    16501 cgggcctacg agaccgagcg cggcacggcg atattccggc acctcgacca tctcgaccgg
    16561 ctcgagcgct cggcgcgctt ctattacatg gacctgccgt tctcgcccgc gaagatgcgc
    16621 gaggcaacac acgagttgat cgcccgcaac gggttccgct cctgctacat ccgcccgctc
    16681 gtctaccgcg gctacgggcc gatgggcctc gacccgctca ccaacccggt ggaggcgatg
    16741 atcgccgtgt gggagtgggg gagctacctg ggcgaagagg gcaagcgcaa cggcattcgc
    16801 gcgaaggtgt cgtcctggcg gcgtatctcg cccgactcgc tctacccgca cgcgaaggcg
    16861 tcgggccagt acctgaactc ggtgctcgcg aagatcgagg cctcgaaggc cgggtacgag
    16921 gaggcgatcc tcctcgacca caacggcttc gtgtgcgagg gcagcggcga gaacatcttc
    16981 gtcgtgaagg acggcgtcat ctacacgccg ccgcagagca cgtcgatcct ggacgggatc
    17041 aaccgcaagt cgatcatcac gatcgcccac gatttcggcc tcgaggtggt ggagcgcgac
    17101 atcgcgcgcg gggagctgat cctcgcggac gaggtgttca tgagcgggac agccgccgag
    17161 ctcactccgg tgcgcgagat cgacgactac ccgctcgggc ctcccggcga gatcacccgc
    17221 agggtccagg tgaagttcga ggacgcgctg tacgggcgcg cgcccgagta cgccgagtgg
    17281 ctcgacatcg tgcaggtacc atcgaaggcg tgaacgccgc cttcgagcac gggcgaatta
    17341 cctcggcggc ctcgccgcgc tagcgcgcgg gtcgccgcag gcacccgttc tgtccgctcg
    17401 aatcctgttt cccgaaaggc gttcaaatgg ctgaagcagt agtcctctac gacaccaccc
    17461 tccgcgacgg catgcagggc gagggcatgt cgctctccgc agaggagaag gtgcgcgtgg
    17521 cgcacgcgct cgactcgctc ggcgtgccgc tgatcgaggc cggcttcccc agctcgaacc
    17581 cgaaggagca ggcgctcttc gacctgctgt cggcggagcg cttcgagcac gcggaggtcg
    17641 ccgccttcgg gatgacgcgc cggcgcggcg tcagcgcgga ggacgacccc gcgctgcgcc
    17701 tgctcgcgga ctgcttcgcg ccggcgtgca cgctggtggg gaagacctgg gcgctgcacc
    17761 tcgagaaggt cacgcgcgtg gaccgggatg agaacctggc gatgatcgcc gactcggtcg
    17821 ccttcctgcg cggctcgggc aagcgcgtga tctacgacgc cgagcacttc ttcgacgcct
    17881 tccgcgagga ccccgactac gccctgcgct gcctgcgcac cgcgctcgag gcgggggccg
    17941 agaacgtcac gctctgcgac acgaacggct cgtccctgcc gcatcaggtg gccgaggcgg
    18001 ccgcgcgcgt agtgggagag ttcggcgacg gctcgcaggt gggcatccac acgcatgacg
    18061 acgccggctg cggggtcgcg aactcgctgg ccgcggtaga cgccggtgcg cggctcgtgc
    18121 agggcacgat gaacggctac ggcgagcgct gcggcaacgc caacctcaca tcgatactgc
    18181 ccgcgctcca gctcaagatg ggctacgact gcgtcgcgcc ggagctgctc gcacggctca
    18241 ccgaggtagc gcacctggtc gacgagatct gcaacgtcac gccgaacgcg aaccagccgt
    18301 acgtgggcaa gaacgccttc gcgcacaagg gcggcatgca cgtcgcgggc gtgctggccg
    18361 actcgcgcac gttcgagcac atggaaccgg cggcggtggg caacgatcgc tcggtgctcg
    18421 tatcggagct gtcgggcaag ggcacggtgc aggcgcgcgc ggagcagtcc gggctcgacc
    18481 tcgacgacgc caccgcgctg cgcgtggtgg agcgcgtgaa ggacctcgag caccgcggct
    18541 tccagttcga ggcggccgac ggctccttcg atctgctgat ccggcgcgag acgggcgact
    18601 ggacgccgct cttccgcctc gagtcgtggc gcgcgatcgt ggagaagcgc gaggacggca
    18661 aggtgatgac cgaggccacg atcaagatct gggtcgaggg tgagcgctac gtgcgaacgg
    18721 ccgaggggaa cggcccggtg aacgcgctcg acaaggcgct ccgcgacgcg atcggcgagc
    18781 actacccgca cctgcgggac gtgcgcctcg tgaacttcaa ggtgcgaatt ctcgacgaga
    18841 cgaagggcac cggcgctgtc acgcgcgtgc tgctcgacgc ctccgacggc accgacacgt
    18901 ggggctcgat cagcgtgtcc gagaacatca tcgaggcgag ctgggaagcg ctcgtcgatt
    18961 ccctggaggc gggcatgctg ccgggccgca ccgagcacgt gcgccacagc gcgcccgccg
    19021 agcccgccgc accgtgagcc aggagccgat cccgctcgcg aagccgctga tcggcgagcg
    19081 cgaggagcag ctcgtgctcg acacgcttcg gtcgggacgc ctgtcgctcg gcccgcgcct
    19141 cggcgagttg gaggacgcct tcgcgcagcg gctcggcgtg ccgagcgtgt ccgcggtgtc
    19201 gagcggcact gccggcctcc acctcgccgt gcgcgccgcc ggcatcgagg ccggcgacga
    19261 ggtcatcacc acgccgttca gcttcgtcgc ctcggcgaac tgcgtgctgt acgagaacgc
    19321 gcggcccgtg ttctgcgaca tcgacccgcg cacgctgaac atagacggcg aggccgcggc
    19381 cgccgcggtc accgaacgca caaccggcct gctgcccgtg cacatcttcg gctatcccgc
    19441 cgacctgccg gcgctcgagc gaatcggggc gcagcgcggc ctctggatcg tggaggacgc
    19501 ctgcgaggcg ctcggcgccc gccacgccga cggcaagccg gtgggcgcgc gcggccatca
    19561 ggcggtgttt gccttctacg ccaacaagca gatcaccacc ggcgagggcg ggatggtcgt
    19621 ctccgagcac aaggagaaga tcgagtccga gcgcaaccag ggccgcgccc cggacatggg
    19681 ctggctcgac cacgaccggc tcggcttcaa ctaccggctc accgacatcg cgtgcgcgct
    19741 cggcatcgcg cagctcgagc ggctcgacga cctcctcgac ggtcgcgcgg ccgcggcggc
    19801 gctgtactcc ggcgcgctcg ccgagatcga gggcctcgag ctcccgtgtc cggactcggg
    19861 cggcgacgtc cgcggctggt tcgtgttcgt ggtccagctg ccggagggcc gcgaccgcga
    19921 cgccacgatc ctcgcgctgc gcgagcaggg cgtgcagtcc aagcccta
//
LOCUS       DASVXS010000293         7683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_772084, whole genome shotgun sequence.
ACCESSION   DASVXS010000293 DASVXS010000000
VERSION     DASVXS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772084"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VF752_16445"
     CDS             <1..1016
                     /locus_tag="VF752_16445"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX8857186.1"
                     /translation="DPVVDSASIIQSLRERAREEARVPTGFLAAVTVGQEGELLTEMG
                     ELMGAGAFGFTDDGLPVASAGVLRQALRYQRMSGGCIALHEEDPTLSRGGSMHEGQVS
                     ALLGVGGVPSISESTMIARDAAIARYEDAQIHIQHLSARESVEAIEQAKAVGVRVTCE
                     ATPHHLSLTDESVRALDTNYKMNPPLRSEDDRQALLDGLRSGIIDCVATDHAPHSREE
                     KEQPFEAAPMGVTGLETAFAALHTLLVTPGLIPLAVLIERMTAGGAPFGLAIPSLSPG
                     SPANVCLADLAAEWEAGEAGYESRSSNSCFAGRTLTGRVRMTVAGGSVAYRERSFAIG
                     AVS"
     gene            1019..1561
                     /locus_tag="VF752_16450"
     CDS             1019..1561
                     /locus_tag="VF752_16450"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX8857187.1"
                     /translation="MPAKRLLDRERAALLVVDVQEAFRPVIRKFDLVAGQVALLVQGA
                     HAVGIPVLASEQYPKGLGHTVPEVAQHLNGAAPIEKVCFAATDAEGFDLQGRDQVIIC
                     GIEAHICVSQTAHALLDRGVEVHLAADACGARTKQNLRTGLLKMTTAGAIPSSVETAL
                     FELLGAAGSEEFKAIQALVK"
     gene            1558..2682
                     /gene="carA"
                     /locus_tag="VF752_16455"
     CDS             1558..2682
                     /gene="carA"
                     /locus_tag="VF752_16455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX8857188.1"
                     /translation="MSTYLLLEDGTRFDGESAGADTFATGEVVFNTAMSGYQESVSDP
                     SYRGQIIVFTYPLIGNYGVSRDAMESDSVHARAVVMREGVNREDAPGAEGGWLTWLSD
                     CRVPALSGIDTRALVRHIRDKGAMRGGVFPGSMPESEAMERVMAEPEMAGADLAREVT
                     PREPLILDPDNPGPRVVALDTGIKDSIMANLRQRGARLELHPCTASAEDLLARDPDAV
                     FLANGPGDPAALDYVVDAVRGCVGKVPVFGICLGHQLLCRAVGLETFKLPFGHRGANH
                     PVKDLETGRIDITSQNHGFAVLGPSGAKTIDADEPVRWETDFGEAELSQLNLYDRTVE
                     GLVLRDVPGATVQYHPEAGPGPHDATHLFDRFMEMIRAAA"
     gene            2669..5824
                     /gene="carB"
                     /locus_tag="VF752_16460"
     CDS             2669..5824
                     /gene="carB"
                     /locus_tag="VF752_16460"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX8857189.1"
                     /translation="MPRRDDLRRILVLGSGPIVIGQAAEFDYSGAQACKVLLEEGYEV
                     VLVNSNPATIMTDPEFATATYIEPLTPGSVARVIERERPDALLPTLGGGTALDLARAL
                     SEDGTLERFGVELIGANYEAIRRAEDRELFRATIEAAGLRVPRSGIARSIAEAEHVLA
                     DVGLPAIVRPGFTMGGRGGGIARSEAEFHQIVGEGLAASPIGQVLIEESVIGWGEFEL
                     EVMRDRNDNVVIVCSIENIDPMGVHTGDSVTVAPQQTLTDKQYQALRDQAIAVIRAVG
                     VETGGSNIQFAVNPETDEIVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGYALEE
                     IDNDITKATPASFEPTIDYVVVKWPRFAFEKFPGTSGKLSTHMQSVGEAMSIGRTFKQ
                     AMVKAMRSRELDVTPPTDVPLEELLARLETPSHDRFEVLFAALRAGASEQEIHRRTHI
                     DPWYVSELAELAAGVDPEDGLARTFKSVDTCAAEFEAATPYFYSGLERPAPDGAARHE
                     VEPGERPAVVILGAGPNRIGQGIEFDYCCVHAAMTVRESGRDAVMINCNPETVSTDYD
                     TSDRLYFEPLTLEDVLGVIEVEQPEGVIVQFGGQTPLKLAKGLHDAGVPLLGTPVDSI
                     DLAEDRARFGALIRELELKSPPYATAESAEGALEAAEQVGYPLLVRPSYVLGGRAMEI
                     CYSPEQLADYLRRTGAANGKGTTIYLDRFLENAIEIDVDALCDGDEVRIGAIMQHVEE
                     AGVHSGDSACVIPAMSLGPEMLRQVEHATEQLALRLGVVGLINLQFAVHADDELYVIE
                     ANPRASRTVPFVSKAVGVPLAKVACRLMLGEKLADLDLALPEPGRGPGHVSVKEAVLP
                     FDRFPHADALLGPEMKSTGEVMGVAADYPTAFGKAQAAAGAGLPIEGSVFISVTDSDK
                     PAATQLAASLHDLGFKVLATGGTAQAIRRMGVPVERIPKISEGSPNVVDRIESGDVAL
                     VINTPTGSGARSDGYEIRRAAVARGVPCITTMTGATAAQRAIRALQLGEPAVRSLQEL
                     HASARKHVALRREAAPADVPG"
     gene            5821..6618
                     /locus_tag="VF752_16465"
     CDS             5821..6618
                     /locus_tag="VF752_16465"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX8857190.1"
                     /translation="MTAPFGRRLTTVSQGRDVGAYRVITAVDREGPDPEPGQFYMLAA
                     AERWGGGESERPWLPRAFSYARARPAHGGGVELDFLLEAVGPGTERLAELERGDGLWL
                     TGPLGVGFSPADRPLLVGGGIGAAPLLCLQDQHPDAPVLLGFRSAAYAEAAALFEGPV
                     GLCTDDGSAGRQAFVTELLRERLDSGEGQTVYACGPPAMLEAVRALCAEREVPAQLAL
                     ESGMACGFGACFGCVVPTRSGYVRLCVDGPVLDAAELVTAEVPGAGH"
     gene            6615..7541
                     /locus_tag="VF752_16470"
     CDS             6615..7541
                     /locus_tag="VF752_16470"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX8857191.1"
                     /translation="MSRSIELCGVELRGPVLNGSGTFDAIAAHRAFGDELYARFPFDA
                     FVSKTITVEPREGNPLPRLWETPAGLINSIGLPNKGLDGFLASDLPRLAEVPVPLVVS
                     VMGFSVSELKRLVTAVAARDEVAMLELNFSCPNVETGLIMGADPGETARAVERLRPLT
                     EKPLIVKLTPNATDPAAVAAAAEGTGADGLSLINTLKGMAIDPRTGRPWLGATTGGVS
                     GPAVKAIALQQVHQVSARVKLPLIGMGGIQSGRDAADFLLAGATAIAVGTENFRDPAA
                     GSRVAAELEAVFRNEREYLGEWAPPMSGRGSQ"
BASE COUNT         1120 a         2652 c         2804 g         1107 t
ORIGIN      
        1 ccgacccagt cgtggactcg gcgtcgatca tccagtcgct gcgcgagcgt gcgcgcgagg
       61 aggcgcgtgt gccaacgggc ttcctcgctg cggtgacggt cgggcaggag ggcgagctgc
      121 tcaccgagat gggcgagctc atgggcgccg gcgctttcgg cttcaccgac gacggcctgc
      181 cggtcgcgag cgcgggcgtg ctgcgccagg cgctccgcta ccagcgcatg agcggtggct
      241 gcatcgcact acacgaggag gacccgacgc tctcgcgcgg cggctcgatg cacgagggac
      301 aggtgtcggc gctgctcggc gtcggcggcg tcccgtcgat cagcgagtcg accatgatcg
      361 cccgcgacgc cgcgatcgcc cgctacgagg acgcgcagat ccacatccag cacctctcgg
      421 cgcgcgagtc ggtggaggcg atagagcagg cgaaggcggt cggcgtgcgc gtcacctgcg
      481 aggccacgcc gcaccacctc tcgctcaccg acgagtccgt gcgcgcactc gacacgaact
      541 acaagatgaa tccgccgctg cggtcggagg acgacaggca ggcgctgctc gacgggctgc
      601 gatccggcat catcgactgc gtggccaccg accacgcgcc gcactcccgc gaggagaagg
      661 agcagccgtt cgaggccgcg ccgatgggcg tgaccggcct cgagacggcg ttcgccgcac
      721 tgcacacgct tctggtgacg ccgggcctga tcccgctcgc cgtgctaatc gaacgcatga
      781 ccgcaggcgg cgcgcccttc gggctggcga tcccgtcgct ctcgcccggc tcgccggcga
      841 acgtctgcct cgccgacctc gcagctgagt gggaagcggg cgaggccggc tacgagagcc
      901 gctcctcgaa ctcctgcttc gccgggcgca cgctcaccgg acgcgtgcgc atgacggtcg
      961 ccggtggctc ggtcgcctac cgcgagcgct cgttcgcgat cggggcggtg agctgagcgt
     1021 gcctgcgaag cggctgctcg accgagagcg cgcggcgctg ctggtcgtgg acgtgcagga
     1081 ggcgttccgg ccggtgattc gcaagttcga cctcgtggcg ggccaggtcg cgctgctcgt
     1141 ccaaggcgcg cacgccgtcg gcatcccggt gctggcgagc gagcagtatc cgaaagggct
     1201 cggccacacc gtgcccgagg tcgcgcagca cctgaacggc gcggcgccga tcgagaaggt
     1261 ctgcttcgcg gccaccgacg ccgagggctt cgacctccag ggccgcgacc aggtgatcat
     1321 ctgcgggatc gaggcgcaca tctgcgtgtc gcagacggcc cacgcgctgc tcgaccgggg
     1381 tgtggaggtc cacctcgccg ccgatgcctg cggcgcccgc accaagcaga acctgcgcac
     1441 cggcctgctc aagatgacca ccgccggcgc gatcccgtcg agcgtggaga cggcgctgtt
     1501 cgagctactc ggcgccgccg gcagcgagga gttcaaggcg atccaggcgc tggtgaagtg
     1561 agcacctacc tgctgctcga ggacggcacc cgcttcgacg gcgagtccgc cggcgccgac
     1621 acgttcgcga ccggcgaggt cgtgttcaac accgcgatgt cgggctacca ggagtcggtg
     1681 agcgacccga gctaccgcgg ccagatcatc gtcttcacct acccgctgat cgggaactac
     1741 ggcgtgtcgc gcgacgcgat ggagtcggac tccgtgcacg cgcgggcggt ggtgatgcgc
     1801 gagggcgtga accgcgagga cgcaccgggc gcggagggcg gctggctcac gtggctgtcc
     1861 gactgtcgcg tgccggcgct cagcggcatc gacacgcggg cgctcgtgcg ccacatccgc
     1921 gacaagggcg cgatgcgggg cggcgtgttc ccgggctcga tgcccgagtc ggaggcgatg
     1981 gagcgcgtga tggcagagcc ggagatggcg ggcgccgatc tcgcgcgcga ggtgaccccg
     2041 cgcgagccgc tgatcctcga tcccgacaac ccgggtcccc gcgtcgtcgc gctcgacacg
     2101 ggcatcaagg actcgatcat ggcgaacctc cgacagcgcg gcgcgcggct cgagctgcac
     2161 ccgtgcaccg cgagcgccga ggacctgctc gcacgcgacc ccgacgcggt gttcctcgcg
     2221 aacggtcccg gcgacccggc ggcgctcgac tatgtcgtcg acgcggtccg cggctgcgtc
     2281 ggaaaggttc ccgtgttcgg catctgcctg gggcaccagc tgctctgccg cgcggtcggc
     2341 ctcgagacct tcaagttgcc gttcggccac cgcggcgcga atcacccggt gaaggacctc
     2401 gagaccggtc gcatcgacat cacctcccag aaccacggct tcgcggtgct cggcccgagc
     2461 ggcgcgaaga cgatcgacgc cgacgagccg gtgcgctggg agaccgactt cggcgaggcc
     2521 gagctttcgc agctgaacct ctacgaccgg accgtcgagg gtctcgtgct gcgcgacgtg
     2581 ccgggcgcga cggtgcagta tcaccccgaa gcgggccccg gtccacatga cgcgacccac
     2641 ttgttcgaca ggttcatgga gatgatccgt gccgcggcgt gacgacttga ggcgcatcct
     2701 cgtcctcggc agcgggccga tcgtgatcgg ccaggccgcg gagttcgact actcgggcgc
     2761 gcaggcctgc aaggtgctgc tcgaggaggg ctacgaggtc gtgctcgtga actcgaaccc
     2821 ggccacgatc atgaccgacc ccgagttcgc cacggccacc tacatcgagc cgctcacgcc
     2881 gggatcggtg gcgcgggtga tcgagcgcga gcgccccgac gcgctgctgc cgacgcttgg
     2941 cggcggcacc gcgctcgacc tggcgcgcgc gctgtcggag gacggcacgc tcgagcgctt
     3001 cggcgtcgag ctgatcggcg cgaactacga ggcgatccgg cgcgcggagg accgcgagct
     3061 cttccgcgcg acgatcgagg cggcgggcct gagagttccg cgctcgggca tcgcgcgctc
     3121 gatcgccgag gcggagcacg tgctggccga cgtcggactt ccggcgatcg tgcggccggg
     3181 cttcacgatg ggcgggcgcg ggggcggcat cgcgcgcagt gaggccgagt tccaccagat
     3241 tgtgggcgag ggcctcgccg ccagtccgat cggccaggtg ctgatcgagg agtccgtgat
     3301 cgggtggggc gagttcgaac tcgaggtgat gcgcgaccgc aacgacaacg tggtgatcgt
     3361 ctgctcgatc gagaacatcg acccgatggg cgtgcatacc ggcgactcgg tcacggtcgc
     3421 gccgcagcag acgctcacgg acaagcagta ccaggcgctc cgcgaccagg cgatcgcggt
     3481 catccgcgcg gtgggcgtcg agaccggtgg ctcgaacatc cagttcgcgg tcaaccccga
     3541 gacggacgag atcgtcgtga tcgagatgaa cccgcgcgtg tcgcgctcga gtgcgctcgc
     3601 gtcgaaggcg accggcttcc cgatcgcgaa gatcgccgcg cggctggccg tgggctacgc
     3661 gctcgaggag atcgacaacg acatcaccaa ggcaacgccg gcgagcttcg agccgacgat
     3721 cgactacgtg gtggtgaagt ggccgcggtt cgcgttcgag aagttcccgg ggacgagcgg
     3781 caagctgtcc acgcacatgc agtcggtggg cgaggcgatg tcgatcggcc gcacgttcaa
     3841 gcaggcgatg gtgaaggcga tgcgctcgcg cgagctcgac gtgacgccgc cgacggacgt
     3901 gccgctcgag gagttgctcg cccgtctcga gactccatcg cacgatcgct tcgaggtgct
     3961 gttcgcggcg ctgcgtgccg gcgcctccga gcaggagatc caccgccgca cccacatcga
     4021 cccctggtac gtgtcggagc tcgccgagct cgcggccggt gtcgaccccg aagacggcct
     4081 tgcccgcacg ttcaagtcgg tcgacacctg cgcggccgag ttcgaggccg ccactccgta
     4141 cttctactcg ggcttagagc gcccggcgcc cgacggcgcc gcccgccacg aggtggagcc
     4201 cggcgagcgg ccggcggtcg tgatcctcgg cgcgggcccg aaccgcatcg ggcagggcat
     4261 cgagttcgac tactgctgcg tgcacgcggc catgaccgtg cgcgagtcgg gccgcgacgc
     4321 tgtgatgatc aactgcaacc cggagaccgt ttcgaccgac tacgacacga gcgaccgcct
     4381 gtacttcgag ccgctcacgc tcgaggacgt gctcggcgtg atcgaggtcg agcagccgga
     4441 gggcgtgatc gtgcagttcg gcggccagac gccgctgaag ctcgcgaagg ggctgcacga
     4501 cgcgggcgtg ccgctgctcg gcacaccggt cgactcgatc gatctggccg aggaccgcgc
     4561 ccgcttcggc gcgctgatca gggagctgga gctgaagtcg ccgccgtacg cgaccgccga
     4621 gtcggccgag ggcgccctcg aggccgcgga gcaggtcggc tacccgctgc tggtgcgccc
     4681 gagctacgtg cttggcggac gcgccatgga gatctgctat tcgcccgagc agctcgccga
     4741 ctacctgcgc cgcaccggcg cggcaaacgg caagggcacg acgatctatc tcgaccgctt
     4801 cctcgagaac gccatcgaga tcgacgtgga cgcgctctgc gacggcgacg aggtgcggat
     4861 cggcgcgatc atgcagcacg tcgaggaggc aggcgtgcac tccggcgact cggcctgcgt
     4921 gatcccggcg atgtcgctcg gcccggagat gctccggcag gtggagcacg caaccgagca
     4981 gctcgcgctc cggctcggcg tggtgggcct gatcaacctc cagttcgcgg tccacgccga
     5041 cgacgagctg tacgtgatcg aggccaaccc gcgcgcctcg cgcacagtgc cgttcgtctc
     5101 gaaggcggtg ggcgtgccgc tcgcgaaggt ggcctgccgg ctgatgctcg gcgagaagct
     5161 cgccgacctc gacctcgcgc tgcccgagcc gggccgcgga cccgggcacg tgtcggtgaa
     5221 ggaggcggtg ttgccgttcg accgcttccc gcacgccgac gccctgctcg gcccagagat
     5281 gaagtcgacc ggcgaggtga tgggcgtggc cgcggactac ccgaccgcgt tcggcaaggc
     5341 ccaggcagcg gccggcgccg gcctgcccat cgagggctcg gtcttcatca gcgtcaccga
     5401 cagcgacaag ccggcggcca cgcagctcgc ggcgtcgctg cacgacctcg gcttcaaggt
     5461 cctggcaacg ggcggcaccg cgcaggcgat ccggcgcatg ggcgtgccgg tcgagcggat
     5521 cccgaagatc tccgagggct cgccgaatgt ggtcgaccgc atcgagtccg gcgacgttgc
     5581 gctggtgatc aatacgccga cgggctcggg ggcgcgctcc gacggctacg agatccgccg
     5641 tgcggcggtg gcgcgtggcg ttccctgtat cacgacgatg accggcgcga cggccgccca
     5701 gcgcgcgatc cgcgccctgc agctcggcga gcccgcggtg cgttcgctgc aggaactgca
     5761 cgcttcggcg cgcaagcacg tggcgctgcg gcgcgaggcc gccccggccg acgttcccgg
     5821 atgacggccc ccttcggcag gcgcctgacg acggtgtccc aggggcgcga cgtgggggcc
     5881 taccgcgtga tcacggcggt tgaccgggag gggcccgatc cggagccggg ccagttctac
     5941 atgctcgcgg cggccgagcg ctggggcggg ggagagtcgg agcggccgtg gctgccgcgt
     6001 gcgttctcat acgcgcgcgc gaggcctgcg cacggcggcg gggtggagct ggacttcctt
     6061 ctcgaggcgg tcggacccgg cacggagcgc ctcgccgagc tcgagcgggg cgacgggctc
     6121 tggctcaccg gcccgctcgg cgtcggcttc agcccggcag accgcccgct gctcgtgggc
     6181 ggcgggatcg gcgcggcgcc gctgctgtgc ctgcaggatc agcaccccga cgctcccgtg
     6241 ctgctcggct tccgttctgc ggcctacgcc gaggcggccg cactgttcga gggcccggtc
     6301 ggactctgca ccgacgacgg ctccgccgga cgccaggcgt tcgtgaccga gctgctacgc
     6361 gagcggctcg actccggcga aggccagacc gtctacgcct gcgggccgcc ggcgatgctc
     6421 gaggccgtgc gcgcgctctg cgccgagcgc gaggtgcccg cgcagctcgc gctcgagtcg
     6481 ggaatggcgt gcggcttcgg cgcctgcttc ggctgcgtgg tgcccacgcg ctcgggctat
     6541 gtgcgcctgt gcgttgacgg gccggtgctc gacgcggccg agctcgtgac cgcggaggta
     6601 ccgggggcgg ggcattgagc cgctcgatcg agctgtgcgg cgtcgagctg cgcggcccgg
     6661 tgctgaacgg gtccggcacg ttcgacgcga tcgcggcgca ccgcgcattc ggcgacgagc
     6721 tctacgcgcg cttccccttc gacgctttcg tgtcgaagac gatcaccgtc gagccgcgcg
     6781 agggcaaccc gcttccgcgg ctctgggaga caccggccgg cctgatcaac tcgatcgggc
     6841 tcccgaacaa gggcctcgac ggcttcctcg cgagtgatct gcctcggctc gcggaggtgc
     6901 cggtgccgct tgtggtctcg gtcatgggct tcagcgtttc ggagctcaag cgcctcgtga
     6961 ccgcggtcgc cgcgcgcgac gaagtcgcga tgctggagct caacttctcc tgtccgaatg
     7021 tcgagaccgg cctgatcatg ggcgccgatc cgggcgagac cgctcgcgcg gtggagcgcc
     7081 tgcggccact cacggagaag ccgctgatcg tgaagctcac gcccaacgcc accgacccgg
     7141 ccgccgtagc ggccgccgcg gaggggacgg gggcggatgg actgtcgctg atcaacacgc
     7201 tgaagggcat ggcgatcgat ccgcgcaccg gacggccgtg gctgggtgcc accaccggag
     7261 gggtctcggg tcccgccgtg aaggcgatcg cgctgcagca ggtacaccag gtctcggcgc
     7321 gggtgaagtt gcccctgatc gggatgggcg gcatccaatc cggtcgcgac gccgccgatt
     7381 ttctcctcgc cggcgcgacg gcgattgcgg tgggcacgga gaactttcga gacccggccg
     7441 ccggcagccg cgtggcggcc gagctcgagg cggtttttcg caatgagcgg gaatatttgg
     7501 gggagtgggc accgcccatg agcggacggg ggtcacagtg acccaaaaag ggcctgcaat
     7561 tcgccacaaa agcctcaagc agtccgaagc cgactaaacc tcaacctgag gtggaggcga
     7621 attcgcgaat ttctccgcgt ttcggcgctg tcgcgcttgt ttttctgtgt aacatcgccg
     7681 ccc
//
LOCUS       DASVXS010000294        14115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1056823, whole genome shotgun sequence.
ACCESSION   DASVXS010000294 DASVXS010000000
VERSION     DASVXS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 14115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14115
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056823"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VF752_16475"
     CDS             complement(<1..451)
                     /locus_tag="VF752_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8857192.1"
                     /translation="MQQLNNAFSRERYRRSLEQSRERRETARRKRRRLFQRRSVSLVL
                     AGAMTVGGGAALAAGGASKLAGSSQTVSTVLRSGSSGPSVSALQRALGIAADGSFGPQ
                     TLAAVKRYQRAHGLVVDGIVGPQTAGSLGLKLTAVSAPVQTGAGAGSG"
     regulatory      complement(460..624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            748..3150
                     /locus_tag="VF752_16480"
     CDS             748..3150
                     /locus_tag="VF752_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8857193.1"
                     /translation="MASARRSLTKPSQQGTRPTGTLAQEWRSLTRAATFVAILTSPAF
                     FFVLYQRGNWSVLAALAGTAAAVVIFRGLVDVITRRLIPSPSLYEADDAARKEDAIAR
                     RRHWYWRKKFRRVTLLAVLFMIPVSIVAIVNGISLGAAISKTLDGIVAFLPLLLGLGL
                     QLPLFFLINLVILFGPLMFFGIRQMKGYEPGDSDWGVRLEDVRGQAEAKEEVRRVISL
                     WQSGEAFEKAGGKRERGLLFLGAPGTGKTMLSKAIATGFNCPFVTVPGSGFAQTFIGM
                     DVIIVQFLVRKAKKLAAKWGGQCIVFIDEIDAVGMRRAALGGGVGGFATPRFEELAFF
                     GPHGALNPSGDLILETPAWRERMFRERAPEPVNYVPGFYRWLQRGIERIIPGGMMGGM
                     GGSLALNQLLVQMDGIDDPPFLKRSLTKRWNTFLDALYVVPQKIGNVSLRLPPPKPAK
                     HEIYFIGATNAPIESLDPALTRPGRMGRHIWFRTPTQGDRKDIFHLYMSKVDHEPDLD
                     TDRRREELARITNGYSPAMIEQVCSMALTYAHADHRPQFGWDDIVEAMTTVETGTAIG
                     IEYVPDETRAVAIHEAGHAVAGHVYMQGVLSTRLSIRMRSGSLGHHQAIEKEERFSSW
                     RHEEIAKLIWTLGAMAAEHVFYGENSTGVGGDVQSATGRAAWMVGMCAMAPDRVDLSG
                     RVPLRDREKREDEIMKRFERLGDRIMQRGGGAGPMMPDPIAGILGDPDKRRSVMGLLG
                     QAYVAAYNVVLYNRAQVEAVAEVLMERREMHGDEVVDLLDEQILERPEIDVLDEASWP
                     KL"
     gene            3147..4037
                     /locus_tag="VF752_16485"
     CDS             3147..4037
                     /locus_tag="VF752_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857194.1"
                     /translation="MSGEDEHREPEQGDELEPAAGQEVEQSHSTELERRRPAEIEMPG
                     VSAEPYMGRFRFVLGALGGFAVAAIAIAVIIGVGGKPAPGPAWSAWKPHGGNGDKIQQ
                     IAHHIGPTYHLPDGNQLVAVNAGPLALQGAPVAITVRTPTRIDSVDGSNGVLYTLCGL
                     GDKCSIDKGKPSNARGVLLRREALELALYTFRYVDKANIVVALLPPPPGQDPSRALFY
                     RRSDLEPSLEQPLARTLPGPPPSVSAMNQPELANDVLRRTEPNEYAFKLNFGAGGGVE
                     LLLDQSPPQPATKKKSSSGR"
     gene            complement(4039..4692)
                     /locus_tag="VF752_16490"
     CDS             complement(4039..4692)
                     /locus_tag="VF752_16490"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8857195.1"
                     /translation="MGRRGGTEAARRCLRRRVPGDQRHGHAGALVQAGVLAHIRRAFA
                     LWQMSGAVRAAMGALNGIYEVGTDRSWRDRMLVSTGIALGQAACLIGALAVVTLAPLA
                     YGDVGQPLTGLLFVARWGVAALLLLACVWLLVRFGPSERRPVRWASFGSVLVVTAWVL
                     MSIGFGAYLRGVANYGSIFGNLASLVILLTYLYLSAIVFLGGAQVDSLVRDDALRRR"
     gene            4976..5314
                     /locus_tag="VF752_16495"
     CDS             4976..5314
                     /locus_tag="VF752_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857196.1"
                     /translation="MERPEEQAPNDEPYVEEQEEEAAAEAGRIGGYGGEPDWPEEERA
                     VREGGGGEQEGFEEAEDELIENASHGDGDLSRDAFGGEEEIESDRSTADYGEADHVIV
                     EDDADDVEGR"
     gene            complement(5408..6415)
                     /locus_tag="VF752_16500"
     CDS             complement(5408..6415)
                     /locus_tag="VF752_16500"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8857197.1"
                     /translation="MGEKRQVVVVTGATSGVGRAIARRFARGGAHIGLIARDREGLDA
                     ASEEVHEAGSKALPLPSDVADHHAVDAAADAVEDALGPIDVWVNCAMTTVFAFFEDIE
                     PEEYRRATEVTYLGSVWGTRAALKRMMPRDSGTIVQVGSALAYRGIPLQSAYCGSKHA
                     MKGMFESVRTELMHKGSNVRVTMVQLPGLNTPQFDHCRSKMPNHPQPVPPIYQPELAA
                     DAVHWAVRHPRREYFVGGSTVYSIWGNKLAPWFADRYLARTAVKGQQTPKHTGPREGN
                     LFQPIPGRDPGAHGDFDAQSHDRSLQWQLSRRVGELVASASAALSGTRRRTRRRPSAE
                     T"
     gene            complement(6446..6694)
                     /locus_tag="VF752_16505"
     CDS             complement(6446..6694)
                     /locus_tag="VF752_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857198.1"
                     /translation="MARDEELAQVAEERRLAAERRAAEALGRARQARREAELATTDET
                     RLVHEREAEAHERAALRHAEAEELQRHHRDAHGGDSQG"
     gene            6782..7768
                     /locus_tag="VF752_16510"
     CDS             6782..7768
                     /locus_tag="VF752_16510"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8857199.1"
                     /translation="MPRARLLVEDPDLASRLEERTAQTLQDLLLVPIEDVPTGPWSPP
                     ERDGPVGGFIVLDGALAKNASIVDSTTTQLFGPGALVQPGADRDETLLPWRTAWYAVS
                     PTRLAELDERVVAAAWHHPGLAAALLARAAETAADAGFILAASNLSRLDARLLALLWH
                     LADRFGHVATDGMVLHLPLSHRLLAQMVGAQRPSVTTALRQLSERDALARRDDGAWLL
                     LGDPVEALERTLSTSPLRQPVGARPVLPRVREPHHVSQAAFAASLHRPTAKIQFTALE
                     ARLNEQLRRLREDHLKSAQRMTETVEKSQEVRSATAELRGEIIRRRAEQSAA"
     gene            complement(7765..9645)
                     /locus_tag="VF752_16515"
     CDS             complement(7765..9645)
                     /locus_tag="VF752_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX8857200.1"
                     /translation="MCPTRQDPNPSTPAEAEALDELLVFLKGTRGFDFTGYKRTSLER
                     RIRKRMDAVDVSSYGQYLDFLQVHPEEYGDLFNTILINVTDFFRDKPAWDHLAAEVLP
                     KLIHERPPDQPLRVWCAACASGQESYTTAMLLAELMGESALRDRVKIYATDVDQEALN
                     EARLSQYSNDQVKHVPRELLDKYFEQNDHRYTVRPELRHAVIFGRNDLVQDAPISRVD
                     LLVCRNTLMYFNAETQSRILGHFNFALTQSGVLFLGKSEMLITHADLFSPISLKWRVF
                     RKVAQPTLRERIGMIVPGTPRDQLPERYLGLREGALEIGRVAEIVLDRGGFVASVNQE
                     ARALFGITARDIGRPLQDLEISYRPVELRSLLERAYEQERAVVASEIQFRNPGGEERS
                     LEVSVTPVISAERAVLGASVSFEDVTRHVRMKADLERSKRELETAYEELQSTVEELET
                     TNEELQSTNEELETTNEELQSTNEELETMNEELQSSNEELETMNDELRDRTSEINRVN
                     TFLEAILSSLHVGVTVLDPEQRVQIWNAASEDMWGLRSEEVEGAHFLNLDIGLPVEEL
                     RQPLKAALAGEPGDGAVSVEAINRRGRPIIVDVSATPLLAEDGSIYGTILLMAEKRRA
                     VS"
     gene            complement(9649..10677)
                     /locus_tag="VF752_16520"
     CDS             complement(9649..10677)
                     /locus_tag="VF752_16520"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8857201.1"
                     /translation="MARSSPRRDIVVVGASAGGVEALSRLVHGLERDNGLSSLFVVLH
                     VMPTGTSVLPNILDRSGPLRAQVARDGAAIEPGNIYVAPPDRHMLIRDNRVRVTLGAR
                     ENGHRPAIDPTFRSAAETYGPRVVGVILSGSLDDGAAGLAAVKRAGGLAVVQDPRDAL
                     YGDMPASAMAATSVDHVLDVDGIAALLNEVAGSGDDDDEDSEDDVPGHWEPLMDELDQ
                     HETEQPASGFTCPECGGALWAKQEGELLQFKCRVGHVYSPESLVEEQGRSLEAAMWAA
                     LRALEERADLLRRVARRVGRRGGSDEHFQRRIENLEEQAETIRKAVTSMSTDVPSLIQ
                     SAEPATQE"
     gene            10911..11456
                     /locus_tag="VF752_16525"
     CDS             10911..11456
                     /locus_tag="VF752_16525"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HEX8857202.1"
                     /translation="MSDHQEQAIVRAVEERLIHADGDGHAHMDAGELRAAIANAMVGL
                     KKRFYGKGPTKAKTYINDNYIFCVLEGGLTRNEETLVAAGAEDLVRDYRLRFQEVVLG
                     VTKQAIAELTGREVVNAHSQIVFNPDRVFEIFVLDGPPLEARAGSDGRPHADGRPPAD
                     GRPHADGRPPADGRPPATTTD"
     gene            complement(11460..12866)
                     /locus_tag="VF752_16530"
     CDS             complement(11460..12866)
                     /locus_tag="VF752_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX8857203.1"
                     /translation="MIRWTVRWLDERVGTSPGIKKALRYVFPDHWSFMLGEIALYSFV
                     VLVATGIFLTFFYEPSSATVTYHGGYAPLRGLTMSRAYESVVDLSFSVPAGLLIRQTH
                     HWAANVFLVAIVLHLMRIFFTGAFRKPRDINYYIGLTMLTLAVVEGYVGYSLVDDLLS
                     GQGLAIGYSTAMSLPVVGGPLAFLLWGGEFPGADSFFTRLLIIHALIIPVTLAALITV
                     HLTIIVRQHHSQFPGPGRTERNTVGTPMWPAYALRSIGLLFAVTSVLFLLGGLIQINP
                     IWEWGPFDTFLSTNGAQPDWYLGWLIGALRLMPAWEPHIGNVTLVPTPFWGGVLFPLA
                     VFGFLYLWPTIERRFTGDQLRHDLLDRPRDNPVRTATGAAFATWVSAAFFAGSTDRVF
                     LQLGISYEGQVWFWRIGGWLLPLVVYWLVKRICVELARTEVHPLRGWAGSVVTVPAAE
                     GSGYAAVEERTPGPDEVA"
     gene            complement(12863..13882)
                     /locus_tag="VF752_16535"
     CDS             complement(12863..13882)
                     /locus_tag="VF752_16535"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8857204.1"
                     /translation="MRLLRRIWQLLVAFFLGRRLKQPVQPSGAPPAPAPPFDPSKREV
                     RNSPRAERAVAALLWVGAACAVVFAIRYVVAGRDTQLLGLTIGLCFAFLAAAAVVAGK
                     FVVPQETAVEERDQLLREQEVEEVGQLVDEGAEGISRRKLLGAAGVAAGATVGAALVV
                     PAASIGPAIDHRVSETPWFPGRRLVDAKGKPYRPEDIEIGTFYTALPQDADPDDLGSS
                     LVVVKLPQNTLRLPKGRETWAPQGVLAYSKVCPHAGCAVSLYRYPLYQPTSAKPAFTC
                     PCHYSTFDPAAGGKLLFGPAGRALPQLPLMVDAEGYLAAQRDFPEDVGPSWLLTKRNP
                     KGPAK"
     gene            complement(13879..>14115)
                     /locus_tag="VF752_16540"
     CDS             complement(13879..>14115)
                     /locus_tag="VF752_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857205.1"
                     /translation="AEAVRMGPYLMPKFPPSQINQQELDSIARYVVYTRNPDDRGGWS
                     IGHIGPIPEGMVAWLLAGSALVLVIRLIGERNEI"
BASE COUNT         2325 a         4863 c         4799 g         2128 t
ORIGIN      
        1 tgccggagcc tgcgccggca ccagtttgca cgggcgcgct cacggcggtg agcttgagcc
       61 cgagcgaacc cgccgtctgc ggtcccacga ttccgtcgac caccagcccg tgcgcgcgct
      121 ggtagcgctt cacggcggcg agcgtctgcg ggccgaagct gccgtcggcc gcgatgccga
      181 gcgcgcgctg gagcgcggac acggacggcc cgctcgaacc ggagcgcagc acggtcgaca
      241 cggtttgcga gctgcccgcg agcttcgacg cgccgcccgc ggcgagcgca gccccgccgc
      301 cgacggtcat cgcgccagcg agcacgagcg acacgctgcg gcgctggaag agccgccggc
      361 gcttgcggcg cgcggtctcg cgcctctcac gcgactgctc gagcgagcgt ctgtatcgct
      421 cgcgcgaaaa ggcgttattc aactgttgca acgaactgct cctcctcttt cgctgcctac
      481 ggggtgagct gacgggctcg cgcggaaaga ggcgcgctac gccgcactaa gtcggcgatt
      541 cgcccccgga gcgacaccag tgccactcca agtgggtccc ccgttcgcga ccgaatgacg
      601 gccgcgattc ggcgtttcag aggtgtacat aagcagatcg accggcggtg cgtcacatat
      661 gggcgcgatc ctctgcgtgg gcgatcgcga aagatcccct cgaaatgcgc caccagcccg
      721 ctcacgacga gtaacctgag gccttagatg gcctccgctc gcaggagcct cacgaagccg
      781 tcccaacagg gcacgcggcc cacgggcacg ctcgctcagg agtggcgctc gctcacgcgc
      841 gcggcgacct tcgtcgcgat cctcaccagc ccggcgttct tctttgtgct ctaccagcgc
      901 gggaactgga gcgtgctcgc tgcgctcgcc ggcaccgcgg ccgccgtcgt gatcttccgc
      961 gggctcgtgg acgtgatcac gcgccggctg atcccgtccc ccagcctcta cgaggcagac
     1021 gacgccgcgc gcaaggagga cgcgatcgcg cgcaggcgcc actggtactg gcgcaagaag
     1081 ttccgccgcg tgacgctgct cgccgtcctc ttcatgatcc ccgtctcgat cgttgccatc
     1141 gtcaacggca tcagcctcgg cgcggcgatc agcaagacgc tcgacgggat cgtcgcgttc
     1201 ctgccgcttc tgctcggcct cggtctgcaa ttgccgctgt tcttcctgat caacctcgtg
     1261 atcctcttcg ggccgctgat gttcttcggc atccggcaga tgaagggcta cgagccggga
     1321 gactccgact ggggggtgcg tttggaggac gtgcgcggtc aggccgaggc caaggaggag
     1381 gtccgccgcg tgatctccct ctggcagtcg ggcgaggcgt tcgagaaggc cggcggcaag
     1441 cgcgagcgcg gcctgctgtt cctgggtgcg ccgggcaccg gcaagacgat gctgtcgaag
     1501 gcgatcgcca ccggcttcaa ctgtccgttc gtcacggtgc cgggctcggg gttcgcccag
     1561 accttcatcg ggatggacgt gatcatcgtc cagttcctgg tgcgcaaggc gaagaagctc
     1621 gcggccaagt ggggcggcca gtgcatcgtg ttcatcgacg agatcgacgc ggtgggcatg
     1681 cgccgcgcgg cgctcggcgg cggtgtgggg ggcttcgcga cgccacgctt cgaggagctc
     1741 gcgttcttcg gcccgcacgg cgcgctgaac ccgagcggcg acctcatcct cgagacgccc
     1801 gcctggcgcg agcgcatgtt ccgggaacgc gcgcccgagc cggtcaacta cgtgccgggc
     1861 ttctaccgct ggctacagcg cggcatcgag cgcatcatcc cgggcggcat gatgggcggc
     1921 atgggcggca gcctggcgct gaaccagttg ctggtgcaga tggacgggat cgacgacccg
     1981 cccttcctga agcgctcact caccaagcgc tggaacacgt tcctcgacgc gctctacgtc
     2041 gtgccccaga agatcggcaa cgtctcgctc aggctgccgc caccgaagcc cgcgaagcac
     2101 gagatctatt tcatcggcgc caccaatgcc ccgatcgagt ccctcgaccc ggcgctaacg
     2161 cgcccgggcc gcatggggcg gcacatctgg ttccgcactc ccacgcaggg ggaccgcaag
     2221 gacatcttcc acctctacat gtcgaaggtg gatcacgagc cggatctcga tacggaccgt
     2281 cgccgagagg agcttgcgcg cataacgaac gggtactcgc cggcgatgat cgagcaggtc
     2341 tgctcgatgg cgctcaccta cgcgcacgcg gaccaccggc cgcagtttgg ctgggacgac
     2401 atcgtcgagg cgatgaccac ggtcgagacc ggcacggcga tcggcatcga gtacgtgccc
     2461 gacgagactc gcgcggtcgc gatccacgag gccggtcacg cggtcgcggg ccacgtctac
     2521 atgcagggcg tgctctccac tcgcctctcg atccgcatgc gctcgggctc gctcgggcat
     2581 caccaggcga tcgagaagga ggagcgcttc tcctcgtggc ggcacgagga gatcgcgaag
     2641 ctgatctgga cgctcggggc gatggccgcg gagcatgtgt tctacggcga gaactccacg
     2701 ggcgtgggcg gcgacgtaca gagcgcgacg gggcgcgcgg cctggatggt gggcatgtgc
     2761 gcgatggcgc ccgaccgggt ggacctcagt ggacgcgtcc cgctcaggga tcgcgagaag
     2821 cgcgaggacg agatcatgaa gcgcttcgag cggctcggcg accgcatcat gcagcgcggg
     2881 ggcggcgccg gcccgatgat gcccgacccg atcgcgggca tcctcggaga ccccgacaag
     2941 cggcgctcgg tcatgggact cctcggccag gcctacgtgg ccgcctacaa cgtggtgctg
     3001 tacaaccgcg cccaggttga ggccgtcgcg gaagtgctga tggaacggcg tgagatgcac
     3061 ggcgatgagg tcgtcgacct gctcgacgag cagatcctcg agcgcccgga gatcgacgtg
     3121 ctcgacgaag cctcctggcc gaagctgtga gcggcgagga cgaacaccgc gaaccggaac
     3181 aaggagatga gctcgagccc gcggccggcc aggaagtcga gcagtcccac tccaccgagc
     3241 tagagcgccg gcgtcccgcg gagatcgaga tgccgggcgt gtcggcggag ccctacatgg
     3301 ggcgcttccg cttcgtgctc ggagcactcg ggggcttcgc ggtcgcggcg atcgcaatcg
     3361 ccgtgatcat cggcgtcggg ggcaagccgg cgcccggacc ggcctggtcg gcgtggaagc
     3421 cgcacggtgg caacggggac aagatccagc agatcgcgca ccacatcggg ccgacctacc
     3481 acctgccaga cggcaatcag ctcgtggcgg tgaatgccgg gccgctggcg ctccaaggtg
     3541 cgcccgtagc gatcacggtg cgcacgccca cgaggatcga ctcggtggac gggtcgaacg
     3601 gcgtgctgta cacactctgc gggctgggcg acaagtgctc gatcgacaag ggcaagccgt
     3661 ccaacgctcg cggtgtcctg ctccgtcgcg aggcgctcga gctcgctctg tacaccttcc
     3721 gctacgtgga caaggcgaac atcgtggtgg cgttgctccc gccgccgccc ggccaggacc
     3781 cgagcagggc gctgttctac cgccgctcgg atctcgagcc gagcctcgag cagccgctgg
     3841 cgcgcacgct ccccggtccg cctccctcgg tctcggcgat gaaccaaccc gagctcgcaa
     3901 acgacgtcct gcgccgaacc gagccgaacg aatacgcgtt caagctcaac ttcggcgcgg
     3961 gcggcggcgt ggagctgctg ctcgaccagt cgcccccaca gccggccacg aagaagaagt
     4021 cctcttcggg caggtaggtc agcgccggcg cagcgcatcg tcgcgcacga gcgaatcgac
     4081 ctgcgcgccg ccgaggaaca cgatcgccga cagatacagg tacgtgagca gaatgacgag
     4141 cgacgcgagg ttgccgaaga tcgagccgta gttggccact ccgcgcaggt aggcgccgaa
     4201 gcctatcgac atcagcaccc aggccgttac cacgagaacg ctgccgaagc tcgcccagcg
     4261 cacgggccgc cgctcggacg ggccaaagcg aacgagtagc cacacgcagg cgagcagcag
     4321 cagcgccgcg actccccagc gcgcgacgaa gagcaggccg gtgagcggct gcccgacgtc
     4381 gccgtaggcg agcggtgcga gcgtgaccac cgccagcgcg ccgatgaggc acgccgcctg
     4441 gccaagggcg atccccgtgg agacgagcat ccggtcgcgc cagcttcgat cggtgccaac
     4501 ctcgtagatg ccgttgagcg cccccatcgc ggcacgcacg gcgcccgaca tctgccacag
     4561 cgcgaaagcg cgccgaatgt gagccagaac gcctgcttgg acgagagcgc ccgcgtgacc
     4621 gtgtcgttga tcacctggta cgcggcgccg gagacagcgt cgtgcagctt cggtgccacc
     4681 tcgtcggccc acacgctctg caggtcgagg aagccggcga gcgcgaccag gaagagcgcg
     4741 aatggcacga tcgccgtgag gatctggaat gcgatcgcgc tcgccgaggt gagcagatcg
     4801 ttgcgctgga actcacggac cagcttgggc acgacgccgg agacggacgg cagtgatggc
     4861 cggcggcgcc gtgtcgcagt cgccattgcc ttgtagtacc cgcttccggc atggttgtgg
     4921 ctgaaccagg gcagtaagag actgcccggt cccatccccg acgcaggagc acgccatgga
     4981 gcgcccagag gaacaagcgc cgaacgacga gccgtacgtg gaggagcagg aggaagaggc
     5041 cgccgccgag gccgggcgga tcggtgggta cggcggcgag ccggactggc ccgaggagga
     5101 gcgagccgta cgcgagggcg gcggcggcga gcaggaaggc ttcgaagagg ccgaggacga
     5161 gctgatcgag aacgcgtccc acggcgacgg tgacctgagc cgcgacgcct tcgggggcga
     5221 ggaggagatc gagagcgacc gctcgacggc cgactacggc gaagccgacc acgtgatcgt
     5281 cgaggacgac gcggacgacg tagagggccg ctgaagcgcc cggcggtgac atgaaacgcg
     5341 gagcaggagc ggcacacccg cgaagggccg ctccgctacg cggtcgcgtg aatcgcctgg
     5401 ttcgcggtca cgtttctgct gatgggcgtc gccgtgttcg gcggcgtgtt ccggatagcg
     5461 ctgcgctagc gctggcgacg agctcgccga cgcgcctcga aagctgccac tggaggctcc
     5521 gatcgtgcga ctgcgcgtcg aagtcgccgt gcgcgccggg gtcacggccc gggatcggct
     5581 ggaacaggtt gccctcgcgc ggtccggtgt gcttgggggt ctgctggccc ttcacggccg
     5641 tacgggcgag gtagcggtcg gcgaaccagg gcgcgagctt gttgccccag atcgagtaga
     5701 cggtcgagcc gcccacaaag tactcgcgac gcgggtggcg cactgcccag tggacggcgt
     5761 cagcggccaa ctcaggctgg tagatcggag gcaccggctg cgggtggttc ggcatcttcg
     5821 agcggcagtg atcgaactgg ggcgtgttga gccccggcag ctggaccatc gtcacgcgca
     5881 cgttcgagcc cttgtgcatg agctcggtgc gcaccgactc gaacatgccc ttcatcgcgt
     5941 gcttcgagcc gcagtacgcg gattggagcg ggatgccgcg gtaggcgagc gcagagccga
     6001 cctgcacgat cgtgccgctg tcgcgcggca tcatccgctt gagcgccgcg cgtgtgcccc
     6061 acaccgaccc gaggtacgtg acctcggtcg cgcgccggta ctcctccggt tcgatgtcct
     6121 cgaagaacgc gaagacggtc gtcatcgcgc agttcaccca cacgtcgatc ggacccagcg
     6181 cgtcctcgac cgcgtcagcg gcggcgtcga ccgcgtggtg atcggctacg tcagagggca
     6241 gcggcagcgc cttcgatccg gcctcgtgca cttcttcgct cgcggcgtcg agcccctccc
     6301 ggtcgcgcgc gatcagccct atgtgcgcac cgcctcgcgc gaagcggcgc gcgatcgcgc
     6361 gccctacccc ggacgtggcc ccggtgacga cgaccacctg cctcttttcg cccatgccgc
     6421 aagccctacc cggtagacgg gagggttagc cctggctgtc tccgccgtgc gcgtcgcggt
     6481 ggtgcctctg aagttcctca gcctcggcgt gacggagcgc ggcgcgttca tgggcctccg
     6541 cttcgcgctc gtgcacgagg cgtgtctcgt cagtggtcgc gagttccgcc tctctgcggg
     6601 cttggcgagc ccgtcccagc gcctcggctg cgcgccgctc agccgcgagt ctgcgctcct
     6661 cggcgacctg cgcgagctcc tcgtcgcgcg ccatgtcctc attgtcccat cgcagccgat
     6721 tgtcggcgct acgtgctcgg acgcgcaata atgctctcaa agggggtgaa ccttcgtcac
     6781 cgtgccgaga gctcggctac ttgttgagga ccccgatctg gcctcacgcc tcgaagagcg
     6841 aaccgcgcag accctacaag acctgctgct cgtcccgatc gaggacgtgc cgaccggtcc
     6901 gtggagtcct ccggagaggg acggcccggt tggcgggttc atcgtgctcg acggggcgct
     6961 cgcgaagaac gcgagcatcg tagattcgac gacgacccag ctgttcggcc ccggtgccct
     7021 cgtgcagccg ggcgccgacc gggacgagac gctgctcccc tggcgcacgg cgtggtatgc
     7081 agtgtccccc acccgcctcg cggagctcga cgagcgcgtg gttgccgccg cttggcatca
     7141 cccgggactt gctgcagcac tcctcgcccg cgccgccgag acggccgcgg acgcaggctt
     7201 catactcgcg gccagcaacc tgagccggct cgacgcgcgc ctgctcgcac tgctgtggca
     7261 cctcgcggac cgcttcgggc acgtggcgac ggacggcatg gttctgcatt tgccgctgtc
     7321 ccaccgtctg ctcgcgcaga tggtcggggc acaacggccg tccgtgacaa cagcactgcg
     7381 ccagctctcg gaacgggacg cgctcgcgcg gcgggacgac ggcgcgtggc tgctactggg
     7441 cgaccccgtg gaggcgctag agcgcacgct ctcgacgtcg ccgttgaggc agcccgtggg
     7501 ggcgcgcccc gtgctgccgc gggtgcgcga gccgcaccac gtgagtcagg cggccttcgc
     7561 ggcctcgctt caccggccga cggcgaagat ccagttcacc gcgctcgagg cccggctgaa
     7621 cgagcagctg aggagactcc gcgaggacca tctgaagtcg gcccagcgca tgacggagac
     7681 agtcgagaag agccaggagg tgcggagtgc gaccgccgag ctgcgcggcg agatcatccg
     7741 ccggcgagcc gagcaatccg cggcctagga gacggcgcgc ctcttctcgg ccatgagcag
     7801 gatggtgccg tagatcgagc cgtcctccgc aaggagcggt gttgcgctca cgtcgacgat
     7861 gatcgggcgc ccgcggcggt tgatcgcctc cactgaaacg gcgccgtcgc ccggctcgcc
     7921 ggccagtgcc gccttcagcg gctgacgcag ctcctcgacc ggcaggccga tgtccaggtt
     7981 gaggaagtgc gcgccctcca cctcctcgct gcgaagcccc cacatgtcct cagacgcggc
     8041 gttccagatt tgcaccctct gctccgggtc gagcaccgtg actcccacgt gcaggctcga
     8101 gaggatcgcc tcgaggaagg tgttcacgcg gttgatctcg gacgtgcggt cccggagctc
     8161 gtcgttcatc gtctcgagct cctcgttgct cgactggagc tcctcgttca tcgtctcgag
     8221 ctcctcgttg gtggactgca gctcttcgtt cgttgtctcg agctcctcgt tggtggactg
     8281 cagctcctcg ttcgttgtct cgagctcctc gaccgtcgac tgaagctcct cgtacgcggt
     8341 ctcgagctcc cgctttgagc gctcgaggtc ggccttcatc cgcacgtgcc gcgtcacatc
     8401 ctcaaacgac acgctcgcgc caaggacggc gcgctcggca gagatgaccg gggtaaccga
     8461 gacctccagc gagcgctcct cgccgcccgg gttgcggaac tggatttcgc tcgccacgac
     8521 ggcccgctcc tgctcgtagg cgcgctccag cagcgacctc agctcgaccg gccggtatga
     8581 gatctcgagg tcctgcagtg gacgaccgat gtcgcgcgcg gtgattccga acagggcgcg
     8641 cgcctcctgg ttcacactcg ccacgaagcc accgcggtcg agcacgatct cagcgaccct
     8701 gccgatttcc agcgcgccct cgcgaaggcc caggtagcgc tcgggcaact gatcccgagg
     8761 agtgccgggc acgatcatgc cgatgcgctc tcgcagcgtc ggctgtgcga ccttgcggaa
     8821 cacacgccac ttcaggctga tcggactgaa caggtcggcg tgcgtgatca gcatctccga
     8881 ctttccgagg aacaggacgc cgctctgggt cagcgcgaag ttgaagtggc cgaggatccg
     8941 cgactgcgtc tccgcgttga agtacatcag cgtgttacgg cacacgagta ggtcgactcg
     9001 cgagatcggc gcatcctgca cgaggtcgtt acgcccgaag atgaccgcat gcctcagctc
     9061 cgggcgcacg gtgtagcggt ggtcgttctg ctcgaagtac ttatcgagca gctccctcgg
     9121 cacgtgcttc acctggtcgt tcgagtactg cgacaggcgc gcctcgttga gcgcctcctg
     9181 gtccacatcc gtggcgtaga tcttcacccg gtcccggagc gcgctctcgc ccatcagctc
     9241 cgcgagcagc atcgcggtgg tgtaggactc ctggcccgag gcgcacgccg cgcaccacac
     9301 cctcagcggc tggtctggcg gcctctcgtg gatgagcttg ggtagcacct cggcggcgag
     9361 gtgatcccac gccggcttgt cccggaagaa gtcggtgaca ttgatgagga tcgtgttgaa
     9421 gaggtctccg tactcctccg ggtgaacctg caggaagtcg aggtactggc cataggacga
     9481 gacgtcgacc gcgtccatac gcttgcgaat gcggcgctcg aggctcgtgc gcttgtagcc
     9541 cgtgaagtcg aagccgcgcg tgcccttgag gaacacgagc agctcatcga gcgcctcggc
     9601 ctcggcagga gtgctcggat tcgggtcctg gcgggtggga cacatctcct actcctgcgt
     9661 ggccggctcg gcgctctgga tgagcgacgg cacgtcagtg gacatcgagg taaccgcctt
     9721 gcggatcgtc tcggcctgct cttccaagtt ctcgatgcgg cgctggaagt gctcatcgga
     9781 gccgccgcgg cggcctacgc ggcgagccac ccgccgcagc aggtcggcgc gttcctcgag
     9841 cgcccggagc gcagcccaca tcgcagcctc gagcgatcgt ccctgctcct ccacgaggct
     9901 ctcgggcgag tagacgtggc cgactctgca cttgaactgg agcagctcgc cctcctgctt
     9961 ggcccacagc gcgccgccgc actcgggaca ggtgaagccc gacgcgggtt gctccgtctc
    10021 gtgctggtcg agctcgtcca tcaagggctc ccagtgccct ggaacatcgt cctccgagtc
    10081 ctcgtcatca tcatccccgg aacccgccac ctcgttcaag agtgccgcga tcccgtctac
    10141 atcgagtacg tgatcgaccg aggtggcggc catggcactc gccggcatgt cgccgtacag
    10201 tgcgtctctc ggatcctgta cgaccgcgag cccacccgcg cgcttgacgg cggcgaggcc
    10261 cgcggcacca tcgtcaagcg agcccgaaag gatcacgccg acgacgcgcg gcccgtacgt
    10321 ctccgccgcg ctccggaacg tcgggtcgat cgccgggcgg tgcccgttct ctcgtgcgcc
    10381 gagcgtgacg cgcacccggt tgtcccgtat cagcatgtgg cggtctggag gcgcgacata
    10441 gatgtttccg ggctcgatcg cggcgccgtc gcgcgctacc tgggcgcgaa gcgggccgga
    10501 gcggtcgagg atgttcggga gcacgctcgt gccggtcggc atcacgtgaa gcacgacgaa
    10561 cagcgagctg agcccgttgt cgcgctccaa cccgtgtacg aggcggctga gggcctccac
    10621 acctcccgcc gaggctccga ccaccacgat gtcccgacgc gggctcgagc gcgccatggc
    10681 gtgaacacta cgccctattc gccgcgacag aactctttcg tcgagcgcct ccgagaggtt
    10741 gctccgcaac gtgtttgccc tacgcccggg tagaggcggt gactcgcaag gggtaaacct
    10801 caattgcgga tcggaggccg caagctcgca cgcgcctgcc ggcccacccc tctccaaccg
    10861 gtaggcgcgt gtgcgcttca cgagaacatc ggatctaccc cccaagttct atgtctgacc
    10921 accaagagca agcgattgtc agggcagtcg aggagaggct gatccatgcc gacggcgacg
    10981 gccatgcaca catggacgca ggcgagcttc gcgcggcaat cgcgaacgcg atggtgggcc
    11041 tgaagaagcg cttctacggg aagggcccga ccaaggccaa gacgtacatc aacgacaact
    11101 acatcttctg cgtgctcgag ggcggcctca cccgcaacga ggagactctc gtcgcagccg
    11161 gcgccgagga cctcgtgcgc gactaccggc tgcggttcca ggaggtcgtg ctcggcgtca
    11221 cgaagcaggc gatcgcggag ctcacggggc gggaggtcgt gaacgcccac agccagatcg
    11281 tcttcaaccc cgaccgcgtg ttcgagatat tcgtgctcga cgggccgccc ctcgaggcgc
    11341 gtgcaggctc ggatgggcgc cctcacgcgg atggacgccc tcccgcggat ggacgccctc
    11401 acgcggatgg acgccctccc gcagatggac gccctcccgc gacaacgacc gactgacgtt
    11461 caggcgacct catcgggtcc gggggtccgc tcctcgaccg ccgcatagcc acttccctcc
    11521 gcggccggca cggtgacgac cgaaccggcc cagccgcgca gcggatgcac ctcggtccgc
    11581 gcgagctcca cgcagatccg cttcacgagc cagtagacga ccagcggaag cagccagccg
    11641 ccgatccgcc agaaccacac ctggccctcg tacgagatcc ccagctgtag gaagacgcgg
    11701 tcggtcgagc cggcgaagaa cgccgccgac acccacgtcg cgaaagcggc gcccgttgcc
    11761 gtacgcaccg ggttgtcgcg cgggcggtcg agcaggtcgt gtcgcagctg atctcccgtg
    11821 aagcgccgtt cgatcgtggg ccagaggtag aggaacccga acaccgcgag cgggaacaga
    11881 acacctcccc agaacggcgt gggcacgagc gtgacgttgc ctatgtgcgg ctcccacgcg
    11941 ggcatcagcc gcagtgcacc gatcagccag ccgagatacc agtcgggttg ggcgccgttc
    12001 gtggaaagga acgtgtcaaa tggaccccac tcccagatgg gattgatctg gatcagccct
    12061 ccgagcagga agagcaccga cgtgaccgcg aatagcaggc cgatcgagcg cagggcgtag
    12121 gccggccaca tcggggtgcc gaccgtgttt cgctccgtgc gccccgggcc ggggaactgc
    12181 gagtggtgct ggcgcacgat gatcgtgagg tggaccgtga tcagcgccgc gagcgtgacg
    12241 gggatgatca gcgcgtggat gatcagcagc cgggtgaaga acgagtccgc gccgggaaac
    12301 tcgccgcccc agaggaggaa cgccagcggt ccgcccacca cgggcagtga catcgcggtc
    12361 gagtacccga tcgctagccc ttggccggac aacaggtcgt ccaccagcga gtagcccacg
    12421 tagccctcga ccaccgccag cgtgagcatt gtgagcccga tgtagtagtt gatgtcgcgc
    12481 ggcttccgga acgccccggt gaagaagatc cgcatcaggt ggagcacgat cgcgacgaga
    12541 aacacgttcg ccgcccagtg gtgcgtctgg cggatcagca ggcccgcggg gaccgagaac
    12601 gacaggtcca ccaccgactc gtaggcgcgc gacatcgtca gcccgcggag gggcgcatag
    12661 cctccgtggt aggtcacggt cgcgctgctc ggctcgtaga agaaggtgag gaagatcccc
    12721 gtggccacga gcaccacgaa cgaatagagc gcgatctcgc ccagcatgaa cgaccagtgg
    12781 tcgggaaaca cgtagcggag cgccttcttg attcccggcg aggtcccgac ccgctcgtcc
    12841 agccagcgca ccgtccagcg gatcacttcg ccggcccctt cgggttgcgc tttgtgagta
    12901 gccacgatgg accgacgtcc tcggggaagt cgcgctgtgc ggcgaggtag ccctccgcat
    12961 cgaccatcag cggcagctgc ggcagcgccc gccccgccgg cccgaacagc agcttgccac
    13021 ccgccgcagg gtcgaacgtg gagtagtggc acgggcaggt gaaggccggc ttggcgctgg
    13081 tcggctggta gagcggatag cgatacagcg agaccgcgca gccggcgtgc gggcacacct
    13141 tcgagtaggc gagcacgccc tggggtgccc aagtctcacg ccccttgggc agccgcagcg
    13201 tgttctgtgg cagcttcacc accaccagcg aggagccgag gtcgtcgggg tcagcatcct
    13261 gcggcagcgc cgtgtagaag gtgccgatct cgatgtcctc gggccggtag ggcttgccct
    13321 tggcgtccac gaggcgccgg ccggggaacc agggcgtctc cgacacgcga tggtcgatcg
    13381 cgggcccgat ggatgcggcc gggaccacca gcgcagcgcc cacggtcgcg cccgccgcca
    13441 cgccggccgc gccgagcagc tttcgccgcg agatgccctc agcgccctcg tcgacgagct
    13501 ggccgacctc ctccacctcc tgctcgcgaa gcagctggtc gcgctcctcg acggccgtct
    13561 cctgcggcac gacgaacttg ccggccacga cggccgcggc ggcgaggaaa gcgaagcaca
    13621 gcccgatcgt gagaccgagc agctgtgtgt cacgcccggc caccacgtag cgaatggcga
    13681 aaacgaccgc gcacgccgcg ccaacccaga gcagggcagc caccgctctc tcggcacgcg
    13741 gactgttccg cacctcgcgc ttgctcggat cgaacggcgg cgcgggcgcg ggcggcgcgc
    13801 cagacggctg cactggctgc ttcaggcgcc ggcccaggaa gaaggccacg agcagctgcc
    13861 agatccgccg taggagcctc atatctcgtt gcgctccccg atcaggcgga tcaccagcac
    13921 gagcgccgac ccagcgagca accaggccac catgccttcc ggaatgggcc cgatgtgccc
    13981 gatcgaccag ccgccgcggt cgtcgggatt gcgcgtgtac acgacgtagc gcgcgatcga
    14041 gtcgagctcc tgctgattga tctggctcgg cgggaactta ggcatcagat acggccccat
    14101 ccgaaccgcc tcggc
//
LOCUS       DASVXS010000295         5000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_844492, whole genome shotgun sequence.
ACCESSION   DASVXS010000295 DASVXS010000000
VERSION     DASVXS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844492"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF752_16545"
     CDS             <1..220
                     /locus_tag="VF752_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857206.1"
                     /translation="LLEATLPRPERTGMRGHLTPGEAGGCAKGAAAKRLLLVHISDEL
                     DAEWAREEAEGTFGGPVEVATEGAVFEV"
     gene            287..781
                     /locus_tag="VF752_16550"
     CDS             287..781
                     /locus_tag="VF752_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX8857207.1"
                     /translation="MTSQPDLFANFARMRREMDELFGDVMQRRRGPRGRGFSPRVDVY
                     YSDNPPRAVVKVDLAAVDPADVALEIRGRQLVIAGERRPTEEGGRLYQQIEIEHGPFR
                     RVVELGADVDPESAKASYEDGVLRIEIPLRQQEQAVRKVPISGRARSLRPSDPERSNG
                     GDER"
     gene            778..3213
                     /gene="lon"
                     /locus_tag="VF752_16555"
     CDS             778..3213
                     /gene="lon"
                     /locus_tag="VF752_16555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8857208.1"
                     /translation="MTIDLPEGAVGVVEGNGSLPQALPVLPLKDSVTFPDTVTPLAVG
                     QPRSVELVNDVLGGNRMMVMVASRNPDVEVPSPDDLYDVGVVGTVARMMKVPDGSLRI
                     LVQAHQRVRLSDYVATEPYLVARIEPLPDVEEDTPELRALMRNVQNTFGQIVEEVPYL
                     PEELQMALANIDDPSELAHLIAGSLRISTEEKQQLLEEVNVAKRLRRLSEILARELEV
                     ISLGTKIQSQVQSELEQNQREYYLRQQLKAIQQELGETDPEQAEAEELRQQVEQADLP
                     EEVRRVAERELSRLERLPAQAAEHGVIRTYLEWIIDLPWNTATEDDLDLKKARRQLDR
                     DHYDIEKIKDRILEFLAVQKLKPDARSAILCFVGPPGVGKTSLGRSIATAMGRKFERI
                     SVGGVRDESEIRGHRRTYVGALPGTIIRAIRDAGTNNPVLMIDEIDKMGVGFQGDPAS
                     AMLEVLDPEQNSTFRDHYLDLPFDLSHVLFITTANMLDTIPAPLQDRMEIIQLSGYTE
                     AEKLEIAKRYLVPRQVERNGLTPQRIEFSDDALKVIIEGYTREAGVRNLERQIGAVCR
                     KVAREYAEGTRNRKRTIRPRTVTEMLGRRRFVEEAKLKPRQPGVSTGLAWTPVGGEIL
                     FIEATSYPGEGRLQITGQLGDVMRESATAALSWAKANVSKWGQGVDPVWWRENDIHLH
                     VPAGAIPKDGPSAGLAMATALVSLVTRRPVRPDVAMTGEIDLIGQALPIGGLKEKALA
                     AQRAGITTVIAPKLNQPDLDDIPPQLRRGLEFVWVDRIDQVLRIALDGGQDARPARRR
                     PATRRAPQPAASR"
     gene            3275..3751
                     /locus_tag="VF752_16560"
     CDS             3275..3751
                     /locus_tag="VF752_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857209.1"
                     /translation="MAKTTRKAAKAGAGAVAAGKAVTSNPYVQRIAEDEELRDNLRTA
                     FESARKAYERMSNGKGPVKAITEDKKTQKELKEAATSLREAADSLRGAKRRPKRKRRG
                     GLLLVLVGAGLALALSEGLRKKALDLVFGSEEEFEYTSTTTASSAGAPSAPVGSTS"
     gene            complement(3837..4793)
                     /locus_tag="VF752_16565"
     CDS             complement(3837..4793)
                     /locus_tag="VF752_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX8857210.1"
                     /translation="MRVHVVATGGTIAMAGALAAPAHAGEELVAALPGVEEIADVSVE
                     QVANVPGVELSPQLAAEALERAERAVAAGARGAVIVQGTDTIEETAQLGDLLWRHEEP
                     VVVTGAMRPASTPSADGPSNLLDAIRVAVSPQARRAGALVSFDGLVHAGDAAVKSHSW
                     RPAAFSSQVPVGRVREGVVELRTRPERRPPIGTLQAALDGGFERRVPIVAAAAGMDGA
                     PLAIEGAEAIVLIALGAGHVPKAMLPAVDEALERGLPVVACARPHEGGTLRRTYGYEG
                     SESDLERRGVILAGDASPWKARIRVLAALALRLDPRELFHAA"
BASE COUNT          797 a         1785 c         1763 g          655 t
ORIGIN      
        1 gctcctggag gcgacgctcc cccggcccga gcgcaccggg atgcgcggtc acctgacgcc
       61 cggcgaggcg ggcggctgcg cgaagggcgc cgccgccaag cgcctcctgc tcgtgcacat
      121 ctcggacgag ctcgatgccg agtgggcgcg cgaggaggca gagggcacgt tcggcggtcc
      181 cgtcgaggtc gcgacagagg gcgcggtctt cgaggtctag gccctccgcg gcggcggacg
      241 cctgagccgg gggtcgaggc ccgaggcgct ctattctcgg gtagcggtga cctctcaacc
      301 cgacctgttc gccaacttcg cgcgcatgcg gcgcgagatg gacgagctgt tcggcgacgt
      361 gatgcagcgc cgccgcggcc ccagggggcg gggcttctcg ccgcgcgtcg acgtctacta
      421 ctcggacaac ccgccgcgcg cggtggtgaa ggttgacctc gccgccgtcg accccgccga
      481 cgtcgcgctc gagatccgcg gccgccagct cgtgatcgcc ggcgagcgcc ggcccaccga
      541 ggagggcggc cgcctctacc agcagatcga gatcgagcac ggcccgttcc ggcgtgtggt
      601 cgagctgggc gcggacgtgg acccggagag cgccaaggcg agctacgagg acggcgtgct
      661 ccggatcgag ataccgctgc ggcagcagga gcaggccgtg cgcaaggtgc cgatcagcgg
      721 ccgcgcgcgt tcgctgcgcc cgagtgatcc cgagcgctcg aacggcggcg acgagcggtg
      781 acgatcgacc tgcccgaggg cgccgtcggc gtggtagagg gcaacggcag cctgccgcag
      841 gcgctgcccg tgctgccgct aaaggactcg gtcaccttcc cagacacggt cacgccgctc
      901 gccgtcggcc agccgcgttc cgtcgagctc gtgaacgacg tgctcggtgg caaccgcatg
      961 atggtgatgg ttgcctcgcg caacccggac gtggaggtgc cgagcccgga cgatctctac
     1021 gacgtcggcg tcgtcggcac ggtcgcgcgc atgatgaagg tgcccgacgg cagcttgcgc
     1081 atcctcgtgc aggcgcacca gcgtgtgagg ctctcggact acgtcgccac cgagccctac
     1141 ctggtcgcac gcatcgagcc acttcccgac gtggaggagg acacgcccga gctccgcgcg
     1201 ctgatgcgca acgtgcagaa cacgttcggg cagatcgtcg aagaggtgcc gtacctgccg
     1261 gaagagctcc agatggcgct cgccaacatc gacgatccgt cggagctcgc gcacctgatc
     1321 gccggctcac tccggatctc gaccgaggag aaacagcagc tgctcgaaga ggtgaacgtc
     1381 gccaagcggc tgcgccgcct ctcggagatc ctcgcgcgcg agctggaggt gatttcgctc
     1441 ggcacgaaga tccagtcgca ggtccagtcg gagctcgagc agaaccagcg cgagtactac
     1501 ctgcgccagc agctgaaggc gatccagcag gagctcggcg agaccgatcc cgagcaggcc
     1561 gaggcggaag agctgcgcca gcaggtcgag caggcagacc tgcccgagga ggtgcgccgc
     1621 gtggccgagc gtgagctttc gcggctcgag cgtctaccgg cgcaggccgc cgagcacggc
     1681 gtgatccgca cctacctcga gtggatcatc gaccttccgt ggaacacggc caccgaggac
     1741 gacctcgacc tgaagaaggc gcgccgccag ctcgatcgcg accactacga catcgagaag
     1801 atcaaggacc gcatcctcga gttcctagcg gtgcagaagc tgaagcccga cgctcgctcg
     1861 gcgatcctct gcttcgtcgg cccaccgggc gtcggcaaga cgtcgctcgg ccgctcgatc
     1921 gccacggcga tggggcgcaa gttcgagcgc atctcggtgg gtggcgtgcg cgacgagtcg
     1981 gagatccgcg gccaccgtcg cacctacgtg ggcgccctgc cgggcacgat catccgcgcg
     2041 atccgcgacg ccggcaccaa caaccccgtg ctgatgatcg acgagatcga caagatgggc
     2101 gtgggcttcc agggcgaccc ggcgagcgcg atgctcgagg tgctcgaccc cgagcagaac
     2161 tcgaccttcc gggaccacta cctcgacctg ccgttcgacc tctcgcatgt tctcttcatc
     2221 acgacggcga acatgctcga cacgatcccc gcgccgctgc aggaccgcat ggagatcatc
     2281 cagctgtcgg gctacaccga ggcggagaag ctcgagatcg ccaagcgcta cctagtgccg
     2341 cgccaggtcg agcgcaacgg gctcacgccg cagcggatcg agttcagcga cgacgcgctg
     2401 aaggtgatca tcgagggcta cacgcgagag gccggcgtgc ggaacctcga gcgccagatc
     2461 ggcgcggtct gccgcaaggt cgcgcgcgag tacgccgagg gcacgcgcaa ccgcaagcgc
     2521 acgatccggc cgcggaccgt caccgagatg ctcggccggc ggcgcttcgt ggaggaggcc
     2581 aagctgaagc cgcgccagcc gggcgtgtcg accggtcttg cctggacgcc ggtcggcggc
     2641 gagatcctct tcatcgaggc gacctcgtat ccgggcgagg gccgcctcca gatcaccggt
     2701 cagctcggcg acgtgatgcg cgagtccgcg accgccgcgc tctcgtgggc gaaggcgaac
     2761 gtgtcgaagt ggggccaggg cgtggacccg gtgtggtggc gggagaacga catccacctc
     2821 cacgtgccgg ccggggcgat tccgaaggac ggcccgagcg caggcctcgc gatggcgacc
     2881 gctctcgtct cgctggtgac gcgccgtccc gtgcggcccg acgtcgcgat gacgggcgaa
     2941 atcgacctga tcgggcaggc ccttccgatc ggcggcctga aggagaaggc gctcgccgcg
     3001 cagcgcgccg gtatcacgac cgtgatcgcg ccgaagctga accagccgga cctcgacgac
     3061 atcccaccgc agctccgcag gggactcgag ttcgtctggg tcgaccgcat cgatcaggtg
     3121 ctccgcatcg ccctcgacgg cggccaggac gcgcggccgg cgcgccgccg cccggccacg
     3181 cggcgcgcgc ctcagccggc tgccagccgc tgacttcgtt tcccccgctc ctgaacccgg
     3241 gtatgctcgc tccgcaagcg gatcgaggag agccatggca aagacaacga ggaaggctgc
     3301 gaaagccggc gcaggggctg tagccgccgg gaaggcggtc acctcgaacc cctacgtcca
     3361 gcggatcgcc gaggacgagg agctcaggga caacctgcgc acggcgttcg agtccgcgcg
     3421 caaggcgtac gagcgcatgt ccaacggcaa gggccccgtg aaggccatca ccgaggacaa
     3481 gaagacacag aaggagctca aggaggcggc gacttcgctt cgcgaggcgg ccgactcgct
     3541 gcgcggcgcc aagcgtcgcc cgaagcgcaa gcggcgtggc gggctgttgc tcgtgctcgt
     3601 cggcgcggga ctcgccctcg ccctgagcga gggtctccgc aagaaggcgc tcgacctcgt
     3661 gttcggctcc gaagaggagt tcgagtacac ctcgaccacc accgcgtcga gcgcgggcgc
     3721 gccgagcgcg cccgttggca gcacctcgta gagcttcggc ctgcgaacca cttgagcgaa
     3781 gggcgtccct cgggacgccc tttgtcgttc gggcgaagct cctaccggcc gtggaatcag
     3841 gcggcgtgga acagctcgcg cgggtcgagc cgcaacgcga gcgccgccag cacccggatg
     3901 cgcgccttcc atgggctggc gtcgcccgcg aggatcactc cccgccgctc gaggtcggac
     3961 tccgagccct cgtagccgta agtgcgccgg agcgtgccgc cctcgtgcgg gcgcgcgcac
     4021 gcgaccacgg gcaggccacg ctccagcgcc tcgtcgaccg ccgggagcat cgccttcgga
     4081 acgtgtccgg cgccgagcgc gatcagcacg atcgcttcgg ctccctcgat cgccagcggc
     4141 gcgccgtcca tgcccgcagc cgcggcgacg atcggtacgc gccgctcgaa cccgccgtcg
     4201 agcgccgctt ggagcgtccc gatcggcggt cgccgctcgg gccgggtccg cagctcgacg
     4261 actccttcgc gcacgcgtcc gacgggcacc tgggaggaga aggcggccgg cctccagctg
     4321 tgcgacttca cagccgcatc gcccgcgtgc acgagaccgt cgaaggagac gagcgcgccg
     4381 gcgcggcgtg cctggggcga cacggccacg cggattgcgt cgagcaggtt gctcgggccg
     4441 tcggcgctcg gggtgctcgc gggtcgcatc gcgccggtca ccacgaccgg ctcctcgtgc
     4501 ctccagagca ggtcgccgag ctgcgcggtc tcctcgatcg tgtccgtgcc ctgcacgatg
     4561 acggcgccgc gcgctcctgc cgctacggca cgctccgcgc gctcgagtgc ctccgcggcg
     4621 agctgcggcg agagctccac gccggggacg ttcgccacct gctccacgga cacgtcggcg
     4681 atttcctcca cgcccggcag cgccgccacc agctcctcgc cggcgtgcgc cggggcggcg
     4741 agcgcgcccg ccatcgcgat cgtgccgccg gtggcgacca cgtgaaccct catcggcggg
     4801 gcagcgtatc ggcaagcccc gcggaactct ggctggccag ccagctaagc tttccagagg
     4861 tggcagcgcc cgtacagacc cgcccgaacg gcgaccgcat cacgcgccgt ctcgacagtg
     4921 agaaggcaca gcgaatcgta tccgcgatgc ggacgagcgt gggacggcgc ggcgccgccg
     4981 gctccacctt cgaccacgtg
//
LOCUS       DASVXS010000296         6133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_120724, whole genome shotgun sequence.
ACCESSION   DASVXS010000296 DASVXS010000000
VERSION     DASVXS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120724"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VF752_16570"
     CDS             <1..1181
                     /locus_tag="VF752_16570"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX8857211.1"
                     /translation="GARPSPFVEEAREALGCEEELHEEQLFRPAEGLHSTFRILRDEL
                     LDTVSQVGGRLAEMRLDTYIDVSQAVVRYLELLKLAALIERSKQGQSVAEALPEVNDL
                     LLQAATPEQVEAFRLSALDDYLRDTERDERRRAAGAPGVPAEQSLEAFIPRRGDGLML
                     SASDIETYRLCPLKYKFARVFRIPQEPSINQRFGIVVHQVLERFHTQGGGSLDDLMLL
                     FAQSWRRSGFGDSNDDRQFREKAVAALERYWELDRRREGEPVWFERGFAFRLGPHLLR
                     GRVDRVDRLPDGRYELIDYKTGKPKTAAELRNDVQLSLYQMGARESWELDTCAQSYYY
                     VLDNEKVPVAHSEEELERVRTTVATIAEGILAQDFEPTPSYDVCAFCDYRIICPAAEK
                     "
     gene            complement(1182..2063)
                     /locus_tag="VF752_16575"
     CDS             complement(1182..2063)
                     /locus_tag="VF752_16575"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX8857212.1"
                     /translation="MEALSFSHRPALRDPALVCAFSGWNDAAEAASSAVSFLVNAFGG
                     FEVARIDPEEFFDFTAVRPTIRLDEGRTRVIEWPATTIHAAHIPAAERDLVLVQGSEP
                     SLKWRAFTECVIDAARELEAKLVVTLGALLADVPHTRPVAVSGLANDQDLIEKLGLER
                     TSYEGPTGIVGVLHDTCAKAGIPSASLWAAVPHYVAAAPNPKAALALIRRFEGIAKVA
                     VDASALEEAAEDYDRQVNAAVASDPEVKEFVERLEETMDEVDEITMRPQDLPSADSIA
                     RDFQRFLRQRRHEEGDG"
     gene            2145..3029
                     /locus_tag="VF752_16580"
     CDS             2145..3029
                     /locus_tag="VF752_16580"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8857213.1"
                     /translation="MEAATSHAPKAIAEVRAGDELRGIFACTRRDRLTARNGSPYLAI
                     ELRDRSGSIGARVFRDADFVAGTFDRGDLVYAAGKVERFRDELQLELREIRRVEEGEA
                     DPAAFLPTAYRDLTELDGFLEHLAREVYDADYRKLLADFLDDESFRGELRRAPCTRAG
                     HHAYLGGLLEHTVAVATLANEACTLHPRLNSDLLLTAAILHDIGKTREFTYGAAIELS
                     EEGRLLGHVGLGQAMIAERSEGLPQEKELQLSHCVLGHHGPDALPGRRFGSADALALY
                     RLNALDASVKGALEHGLG"
     gene            complement(3026..3925)
                     /locus_tag="VF752_16585"
     CDS             complement(3026..3925)
                     /locus_tag="VF752_16585"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8857214.1"
                     /translation="MGSSKWSAEDLPDLTGRTVVVTGANSGIGRAAAAELARAGARVV
                     LAVRDTAKGGEAAAAMPGTTEVRHLDLADLASVHEFADGWDGDLDILINNAGVMAVPE
                     QRTRDGFEMQIGTNHLGPFALTNLLLPRITDRVTTVASGAHRMGSIRLDDLNWENGGY
                     NRWRAYGQSKLANLLFTLELQRRLSEVGSDVRATAAHPGWAATNLQSRTGSFIQNTAM
                     AIVNRVYAQNEEMGALPTLYAATQDIPGGSYVGPDGVGEQRGHPRLVGRSAAARDEET
                     ARELWELSERLTGVSFPLAPAAT"
     gene            3979..4071
                     /locus_tag="VF752_16590"
     tRNA            3979..4071
                     /locus_tag="VF752_16590"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4012..4014,aa:Sec,seq:tca)
     gene            4161..4676
                     /locus_tag="VF752_16595"
     CDS             4161..4676
                     /locus_tag="VF752_16595"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX8857215.1"
                     /translation="MSDRPVFIYAAAYASPEDARADYKGLLELHDADLVGTYDVAIIS
                     KDEAGKVHVEKHEKPTQHGAWTGIAAGAVLGILFPPSILGAAAVGGVTGGVIGHFARG
                     MSRGDMKELGDLLDEGEAGLVVIGESRVQEQLDKHLARARRSMEKQIEADSKDLEREL
                     KAAEKETAAQA"
     gene            complement(4751..5161)
                     /locus_tag="VF752_16600"
     CDS             complement(4751..5161)
                     /locus_tag="VF752_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857216.1"
                     /translation="MKSHRRLFRFRRSAIALACVGALAVPSLASARPIDEPLTAPSLS
                     PAVHAHPKFSPSERVTNTPPAFHPSASERVTDYKAPASFSEPTSTVTVVKQSDGRALS
                     IALSGAALLIALMGGGYVLVRMHGLRQTPAGGIS"
     gene            complement(5265..5948)
                     /locus_tag="VF752_16605"
     CDS             complement(5265..5948)
                     /locus_tag="VF752_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8857217.1"
                     /translation="MSTGTREAPPESFLAQLTPDELEAFNEVAIRRRFPAGCTLFHEG
                     DESGSVYVLTQGRAKISCRGEGGKRAVLGFPGPGDMLGEVSVFDGHPRSASLAAVDEI
                     EALVVPSRAFRRFVDLHPRVALVIIEMLVTRLRAADRLRVEFAMHDVTGRVARRLLEL
                     AVEFGEPDGSGVRIGLKLSQDELASWAGSSREAVSKSLHVLRDLGWIRTERQRLTVLD
                     MDALREFAR"
BASE COUNT          968 a         2114 c         2170 g          881 t
ORIGIN      
        1 cgggcgcgcg gccgtcgccg ttcgtcgagg aggcgcgcga ggcgctgggc tgcgaggagg
       61 agctgcacga ggagcagctg ttcaggccag cggaggggct ccactccacc ttccgcatcc
      121 tgcgcgacga gctgctcgac accgtgtccc aggtcggcgg acggctggcg gagatgcggc
      181 tcgacaccta catcgacgtg tcgcaggcgg tcgtccgcta cctggagctg ctcaagctgg
      241 ccgcgctgat cgagcggtcc aagcagggcc agtcggtggc ggaggcgctg cccgaggtga
      301 acgacctgct gctgcaggct gccacgcccg agcaggtgga ggcgttccgg ctctccgccc
      361 tcgacgacta cttgcgcgac accgagcgcg acgagcgccg gcgagccgcg ggtgctccag
      421 gcgtgccggc ggaacaatcg ctcgaagcgt tcatcccgcg ccgcggcgac gggttgatgc
      481 tctcggcgtc ggacatcgag acataccggc tctgcccgct caagtacaag ttcgcgcgcg
      541 tcttccgcat cccgcaggag ccgagcatca accagcgctt cggcatcgtg gtgcaccagg
      601 tgctcgagcg cttccacacg cagggcggcg gctcgctcga cgacctcatg ctgctgttcg
      661 cgcagagctg gcgacgctcg ggcttcgggg actcgaacga cgaccggcag ttccgcgaga
      721 aggcggtcgc cgcgctcgag cgctactggg agctcgaccg ccgacgcgag ggcgagcccg
      781 tctggttcga gcgcgggttc gccttccggc tcgggccaca cctgctgcgc ggtcgcgtgg
      841 atcgcgtgga ccggctgccc gatgggcgct acgagctgat cgactacaag accggcaagc
      901 cgaagacggc ggccgagctg cgcaacgacg tacagctgtc gctctaccag atgggcgctc
      961 gcgagtcgtg ggagctcgac acctgcgcgc agagctacta ctacgtgctc gacaacgaga
     1021 aggtgccggt cgcgcactcg gaggaggagc tcgagcgcgt ccgaaccacg gtcgcgacga
     1081 tcgccgaggg gatcctcgcg caggatttcg agccgacgcc atcctacgac gtgtgcgcgt
     1141 tctgcgacta ccggatcatc tgcccggcag ccgagaagta atcagccgtc gccctcctcg
     1201 tggcggcgct ggcgcaagaa gcgctggaag tcgcgcgcga tcgagtcggc cgagggaagg
     1261 tcctgcggcc gcatggtgat ctcgtcgacc tcgtccatcg tctcctcgag gcgctcgacg
     1321 aactccttca cctcgggatc gcttgcgacg gccgcgttca cctggcggtc gtagtcctcg
     1381 gcggcctcct cgagcgcgct ggcgtcgacc gccaccttcg cgatcccctc gaagcgccgg
     1441 atgagcgcga gcgccgcctt cgggttcggc gcagcggcca cgtaatgcgg cacggcagcc
     1501 cagaggctcg ccgacgggat gccggccttc gcgcacgtgt cgtgcaggac cccgacgatg
     1561 cccgtcggcc cctcatagct cgtgcgctcg agaccgagct tctcgatcag gtcctggtcg
     1621 ttggcgaggc ccgacactgc gacgggccgc gtgtgcggga cgtcggcgag cagtgcgccg
     1681 agcgtcacga cgagcttcgc ctcgagctcg cgcgccgcgt cgatcacgca ctcggtgaac
     1741 gcgcgccact tgagcgacgg ctcgctgccc tggaccagaa cgaggtcccg ctcggcggcg
     1801 ggaatgtgcg ccgcatggat cgtggtggcg ggccactcga tcacgcgcgt gcgcccctcg
     1861 tcgaggcgga tcgtgggccg caccgcggtg aagtcgaaga actcctcggg gtcgatgcgg
     1921 gccacctcga acccgccgaa cgcgttcacg aggaacgaga cggccgacga tgccgcctca
     1981 gcggcatcgt tccagcccga aaaagcgcac acgagcgcgg ggtcccgcag ggcgggacgg
     2041 tggctgaagg agagggcctc cacctgattc acggtaccga agccgggaac cgcccacacc
     2101 tgcttggctg cggcgtccgg ccggaacccg aagatgaggc atcgatggaa gcggcgacct
     2161 cacacgctcc gaaggcgatc gcggaggtgc gcgcgggtga cgagctgcgc gggatcttcg
     2221 cctgcacgcg cagggaccgg ctcaccgcgc ggaacggctc gccgtacctg gcgatcgagc
     2281 tccgagacag gagcggatcg atcggtgcgc gtgtgttccg cgacgccgac ttcgtggccg
     2341 ggacgttcga ccgtggcgac ctcgtgtacg cggccggcaa ggtggagcgc ttccgcgacg
     2401 agctgcagct cgagctgcgc gagatccgcc gggtggagga gggcgaggcc gacccggctg
     2461 cgttcctgcc gactgcctac cgcgacctca ccgagctcga cggcttcctc gagcatctcg
     2521 cccgcgaggt gtacgacgcg gattaccgca agctgctcgc cgacttcctc gacgacgaat
     2581 ccttccgcgg ggagctgcgg cgcgctccgt gtacgcgcgc gggccaccac gcctacctcg
     2641 gcgggctgct ggagcacacc gttgcggtgg cgactctcgc caacgaagcc tgcacgctgc
     2701 acccgcggct caactccgac ctgttgctca cggcggcgat ccttcacgac atcgggaaga
     2761 cgcgcgagtt cacgtacggg gccgcgatcg agctgagcga ggagggccgc ctgctcggcc
     2821 acgtgggcct gggacaggcg atgatcgccg agcgctcaga aggcctgccg caggaaaagg
     2881 agctgcagct ctcgcactgc gtgctcgggc accacggccc ggatgcgcta ccgggccgcc
     2941 gcttcggctc ggccgacgcg ctcgcgctgt accgcctgaa cgcgctcgac gcctcggtga
     3001 agggcgcgct cgagcacggc ctcggctagg tcgccgcggg cgccagcggg aagctcacgc
     3061 cggtgagccg ctcggacagc tcccacagct cccgcgcggt ctcctcgtcg cgcgccgcgg
     3121 cgcttcggcc cacgagcctc ggatgccccc gctgctcgcc gacaccgtcc gggcccacgt
     3181 aacttccgcc cggaatgtcc tgggttgccg cgtagagagt gggcagcgca cccatctcct
     3241 cgttctgcgc gtagaccctg ttgacgatcg ccatggccgt gttctggatg aagcttcccg
     3301 tgcggctctg cagattcgtg gccgcccacc cggggtgcgc ggccgtcgcg cgcacgtctg
     3361 agcctacctc ggagaggcgc cgctgcagct cgagggtgaa gagcaagttc gcgagcttcg
     3421 actggccata cgcgcgccag cggttgtagc cgccgttctc ccagttcagg tcgtcgaggc
     3481 ggatgctgcc catgcgatgc gcgcccgacg ctacggtcgt cacgcggtcg gtgatgcgcg
     3541 gaaggagcag gttggtgagc gcgaaagggc cgaggtggtt agtgccgatc tgcatctcga
     3601 agccgtccct cgtgcgctgc tcggggacgg ccatgacgcc cgcgttgttg atgaggatgt
     3661 cgagatcgcc gtcccagccg tcggcgaact cgtgcacgga cgccaggtct gcgaggtcga
     3721 gatgcctcac ctcggtagtg cccggcatcg cggcggccgc ctcgccgccc ttggccgtgt
     3781 cgcgcacggc gagcaccacg cgcgcaccgg cgcgtgccag ctcggcagcc gccgcgcgcc
     3841 cgatgccgct gttggctccc gtcacgacga cggtgcggcc ggtgaggtcg ggcaggtcct
     3901 ccgcgctcca cttcgaggag cccatgggag cactgtaacc agtgtgagct acccgcccac
     3961 gggcctacac tcgcgcgcgg agcggcacgg gtctggtggc ccatccggtc ttcaaaaccg
     4021 gcagggcgtg gcagccccac gcttggaagg ttcgattcct tcgccgctcc gtctcgatca
     4081 tccctttcga gtgatgccgc aggcgcggct cccgcttagc gtgagcgacg gcccaccgtc
     4141 tgaagaaggg atcgtctgcc atgagcgaca ggccggtatt catctacgcg gcggcgtacg
     4201 cctcacctga ggacgcgcgc gcggactaca agggtctgct cgagctgcac gacgccgacc
     4261 tcgtcggcac ctacgacgtc gcgatcatca gcaaagacga agccgggaag gtgcacgtcg
     4321 agaagcacga gaagccgacg cagcacggag cgtggaccgg catcgccgcc ggcgcggtac
     4381 tcgggatcct cttcccgccg tcgatactcg gggcggccgc cgtcggcggc gtcaccggag
     4441 gcgtgatcgg tcactttgcg cgcggcatgt cgcgcggcga catgaaggag ctcggggacc
     4501 tgctcgacga gggcgaggcc gggctggtcg tcatcgggga gtcccgcgtc caagagcagc
     4561 tcgacaagca tctcgcgcgt gcccgcaggt ccatggagaa gcagatcgag gcggacagca
     4621 aggatctcga gcgcgagctc aaggcggccg agaaggagac cgccgcgcag gcctgaccag
     4681 tgcagaagcg aaacgcggcc cccgcccgag ggcgagagcc gcgttccgtg gatgacgccg
     4741 gctgcttgtt ttaggagatc ccgccggcgg gggtctggcg gagcccgtgc atgcgaacca
     4801 gcacgtagcc gccacccatc agcgcgatga gcagggcagc tccggagagc gcgatcgaca
     4861 gcgcgcggcc gtcgctctgc ttgacgaccg taacggtcga ggtcggctcc gagaacgacg
     4921 ccggtgcctt gtaatcagtc acgcgctcag aggcggacgg atggaaggcg ggcggcgtat
     4981 tggtgacacg ctcggagggc gagaacttgg gatgcgcgtg cacggccggc gagagcgacg
     5041 gcgccgtcag aggctcgtcg attggccgcg cggacgcgag cgacggaacc gccagcgcac
     5101 ccacgcatgc aagcgcgatg gcggaccggc ggaagcggaa gagacggcgg tgggacttca
     5161 tttgggacct cctacgagtg gcgagtgacg ggcaaggtac gcccgtgtgg aggcccggtc
     5221 cacgcactgg tgcacgcgcc aactgcgtaa ttgttcacaa aggatcagcg cgcgaactcg
     5281 cgcagggcgt ccatgtcgag cacggtgagt cgctggcgct cggtgcggat ccagccgagg
     5341 tcgcgcagca cgtgcagcga cttgctcacg gcttcacgcg aggaacccgc ccagctggcc
     5401 agctcgtcct gcgagagctt cagcccgatg cgcaccccgc tgccgtcggg ctcgccgaac
     5461 tccacggcca gctcgagcag ccggcgtgcg acccggccgg tcacgtcgtg catcgcaaac
     5521 tccacgcgca gacggtcggc cgcacgcagc cgcgtcacca gcatctcgat gatcacgagc
     5581 gcaactcgcg ggtgcaggtc cacgaagcgc cggaaagcgc gggacgggac cacgagtgcc
     5641 tcgatctcgt cgaccgccgc caggctcgcc gagcgtgggt gaccgtcgaa caccgacacc
     5701 tcgcccagca tgtcgcccgg acccggaaag ccgagcacgg cccgcttgcc gccttcgcct
     5761 cggcaagaga tcttcgccct accctgggtc agcacgtaca cgctgcccga ctcgtcaccc
     5821 tcgtgaaaca gcgtgcaccc ggcgggaaag cgccggcgaa tcgccacctc gttgaatgcc
     5881 tcgagctcat cgggtgtgag ctgagccaga aagctttcgg gtggcgcttc tcgggtgccg
     5941 gttgacacgc cgaggcaagc ctaaaccgct caggggcggc ggggaagagg gcgccgtccg
     6001 aatccgcggc aagactccgc ggcatggcgg ccgagcaatc gcgggagtcg aggcgcgccc
     6061 ccgcgaagcg gcgccgggcg ggcggggttg tgcgcgccgc gaccctgtcg acgctgctgc
     6121 tgctcgccgt ggc
//
LOCUS       DASVXS010000297         7542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_775152, whole genome shotgun sequence.
ACCESSION   DASVXS010000297 DASVXS010000000
VERSION     DASVXS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775152"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF752_16610"
     CDS             <1..362
                     /locus_tag="VF752_16610"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8857218.1"
                     /translation="ALARGARPETFAGLAGAGDLVATALAEGSRNRRAGELLARGFSA
                     EEIPAVIHETAEALGSAALLARQLDEAGIEAKATVRLAAMVSGSLPAEEWAAGVHGGR
                     GRFSRSGQDRVEVSTRG"
     gene            372..908
                     /locus_tag="VF752_16615"
     CDS             372..908
                     /locus_tag="VF752_16615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8857219.1"
                     /translation="MATTADKVELDREFSELYRAHLRDVYSYSYYRIGNHHDAEDLTE
                     QTFLQAYRHFERAQRESNGRPLRPWLIRIAHNLAANYHRDRSRRPQTQLEDASVLSEP
                     HATAELVEQREELGHVLAGVADLPDDRREALIMRFALGMDNREIARTLGRSEGATKVL
                     IHRAIKQLERSLEEDDDE"
     gene            901..1227
                     /locus_tag="VF752_16620"
     CDS             901..1227
                     /locus_tag="VF752_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857220.1"
                     /translation="MSEMERLLRSALVPVDPPGTLVDRLEARLTEQLDAAAEELADWE
                     LRSMRNPRNWVRPVAASVALGLAGGALVVVRTRQQQKRRSATGVKAIQKSVNEVAGDV
                     RRRLNR"
     gene            complement(1224..1667)
                     /locus_tag="VF752_16625"
     CDS             complement(1224..1667)
                     /locus_tag="VF752_16625"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX8857221.1"
                     /translation="MREELPKSSAQRYARVAGWLLVALGIAGFFYNSSFATGHETLTN
                     RDAVFGAFDVNGWHNLLHLLAGLIGVRVARSYAGSRAYAYAAGLFFLLLALLGALYGD
                     GDSIFKLFPVNTGDDVLHLVLGLAGLLAATLTSPTPAPSHVARTG"
     gene            1740..2315
                     /locus_tag="VF752_16630"
     CDS             1740..2315
                     /locus_tag="VF752_16630"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8857222.1"
                     /translation="MPRTKELQRLADEELMQLVQDGDPRAFELLYDRHGDAAFSLAYR
                     MVGDRVAAEDITQEAFLSIWRSRERYQRERGSVRTWVLGIVHHRGIDALRRNLVHQRR
                     RASAEGIEERHEAPERTDAEALRRAEAKTVHAALETLPDDQSQVIELAYFGGFTHIQI
                     AEMLAMPVGTVKGRMRLGLEKMRRELAGGVA"
     gene            2312..3028
                     /locus_tag="VF752_16635"
     CDS             2312..3028
                     /locus_tag="VF752_16635"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX8857223.1"
                     /translation="MTMEQHERYAENVAAYLLGALTEEEAAEFEAHLAECELCRLDYQ
                     RLRVAAEALPRAVEPLEAPDSLRDSLMDTVRAEARPGSVAERRPSFLQRLRDRLGEVS
                     PAVAAVSAAAVLAIGVLGGWGVSELANNGTGEKTYAASTTLPRGSATLTVPEDRSGAS
                     LTVRGMPPPGAGRVYEVWLRRGSTYAPAGSLFDVGRNGTGSAAIPGNLRGVDGVAVTR
                     ERRGGTLKPTEKPVMTVNLS"
     gene            3144..3869
                     /locus_tag="VF752_16640"
     CDS             3144..3869
                     /locus_tag="VF752_16640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8857224.1"
                     /translation="MTPTSVGMRCPECSRERTRVHRARTMTDEPRLTYALVAINVIVF
                     LAEFFSGVSAGGGSFGGSDLIRQGALYGPDIANGEVYRLLTAGFLHAGFAHLFFNMYS
                     LYILGSLLEPAVGRLRFAIIYFVSLFAGSFGALIAAPTTLTVGASGAIFGMLGATLVV
                     MRSRGVSAMESGLGLWLLLNLGITFLIPGISIGGHIGGLVGGTLAALAIYEAPRLRVP
                     RTAALVLASLVGVIAIAGSIAVA"
     gene            complement(3884..4162)
                     /locus_tag="VF752_16645"
     CDS             complement(3884..4162)
                     /locus_tag="VF752_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857225.1"
                     /translation="MSSRARITSYGSAAAVVLAGALCVIFISGITGQVLGITLITVGL
                     GAVVLLVFFEVGLSEDRELAAEEQRRRKRMPKHDDGQRPPRRRPRRRG"
     gene            complement(4179..4613)
                     /locus_tag="VF752_16650"
     CDS             complement(4179..4613)
                     /locus_tag="VF752_16650"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8857226.1"
                     /translation="MGAKKAEQTMQIAAAPEDCFTALTEYEEFPEWQRAVKSCEVVTR
                     YEDGRAHHVAFQIDAKVKTVSYTLAYSYEEPHRITWDFVEGDVKDVDGELLLEGDGDS
                     TLATYRVALDPGMWVPGPVKRMVEDQVMRGSLEDLKRRVEGG"
     gene            complement(4616..5611)
                     /locus_tag="VF752_16655"
     CDS             complement(4616..5611)
                     /locus_tag="VF752_16655"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX8857227.1"
                     /translation="MASPSRLQPRSSPSWVCTSSSLRSAIVSSVRIASLVPSATEMLF
                     ALGLGDSVVGVTHECDYPPEAESRPHLTRSVIPDGLSTEEIDRAVRERTQRGEAIYEL
                     DVEKLRELEPDLIVTQALCEVCAVSYDDVRAIAEELPTHPRVLSLDPTTLGEVLNDVR
                     RLAEETEDDEAGDWLLEDAADRIDRVREAVDGAEPVLVAALEWLAPPYVGGHWVPQMI
                     ELAGGVDVIGLPGEKSRTVSWDELRAAKPRVVVSMPCGYYAERAAQEVMDHRSEVESL
                     GAERIVAVDAAAYFSRPGPRLVDGVELLAHILHPDRVESPPPERFIEIDIHARAG"
     gene            5535..5813
                     /locus_tag="VF752_16660"
     CDS             5535..5813
                     /locus_tag="VF752_16660"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX8857228.1"
                     /translation="MALLSDEDVQTQLGELRGWSREGDAIKRQFTFDDFKGSIDFVNR
                     LTPEAEGMNHHPDLEISWNKVTVTLSTHSQGGLTANDFELARRIDSVA"
     gene            5907..7256
                     /locus_tag="VF752_16665"
     CDS             5907..7256
                     /locus_tag="VF752_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX8857229.1"
                     /translation="MPVVAVLLIAVPALIAVNAFFVAAEYALVRARLDRIEALEEEGA
                     SGAILAHRQIDHIDEYIAACQVGVTMASLGLGAVGEPAFANLFENWFGGVVSHSAAVV
                     VSAILGYSVLTTLHITFGELVPKLYTVAFAEKVARRVARPLQASSVIFRPISSVLSNV
                     ARLVLRPFGIEAEGLGEEQTTSEDLKFLIARSASGGVLDPGEALMLRGVFHLHEQEAR
                     QVMTPIPAVVTVDVSETVETALRRCVQSGHTRLVVTEDENTDRVRGIVHNNSLVRLLM
                     TRGGDASIEPAIKDAPIVPETKPLDDLLADLQRERASMSVIVDEYGRTAGIVTVEDII
                     EEVVGEISDETDPAGGAVRRLANGDWYVRGHVPITDLIDYGIELPVDSEAYNSVGGYV
                     FGELGRLPKRGDMVETGGYSLRVESVRENRIEAVRIRDHEPHQRNGDALKESVREEG"
     gene            7296..>7542
                     /locus_tag="VF752_16670"
     CDS             7296..>7542
                     /locus_tag="VF752_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857230.1"
                     /translation="MWSVRLTDSQRDAVRALIEAQGEVGPSLGGALEALEFARWDDLP
                     DARLDWERVQKLANLQGIGEADVVWDLAAGLPTPGTAI"
BASE COUNT         1163 a         2571 c         2664 g         1144 t
ORIGIN      
        1 tcgcgctcgc gcgcggggcc cggcccgaga cgttcgccgg cctggcggga gcgggcgacc
       61 tcgtggcgac agcgctcgcc gagggcagcc gcaaccgccg ggcgggcgaa cttctcgcgc
      121 gcgggttcag cgcggaggag atcccggcgg tgatccacga gaccgccgag gcgctcggga
      181 gcgcggcgct tctcgcccgc cagctcgacg aggcgggtat cgaggccaag gccaccgttc
      241 ggctcgccgc gatggtgagc ggcagtctcc cagccgagga gtgggcggcc ggcgtgcacg
      301 gcggcagggg ccggttctca cgttccggcc aggatcgcgt ggaagtaagc acgcgggggt
      361 aggctccgtg ggtggccacc acggccgaca aggtcgagct cgaccgcgag ttcagcgagc
      421 tgtaccgcgc gcatctgcgc gacgtgtaca gctactccta ctaccggatc ggcaatcacc
      481 acgacgccga ggacctcacg gagcagacct tccttcaggc gtaccggcac ttcgagcgcg
      541 ctcagcgcga gtcgaacggg cgcccgcttc ggccgtggct gatccgcatc gcgcacaatc
      601 tcgcggcgaa ctaccaccgc gaccgctcgc gcaggccgca gacgcagctc gaggacgcgt
      661 cggttctgtc ggagccgcac gcgacggccg agttggtcga gcagcgcgag gagctcgggc
      721 atgtgctcgc gggcgtggcg gatcttcccg acgaccgccg cgaggcgctg atcatgcgct
      781 tcgcgctcgg catggacaac cgcgagatcg cacgcacact cgggcgctcg gagggcgcca
      841 cgaaggtgct gatccacagg gcgatcaagc agctggaacg ctcgctcgag gaggacgacg
      901 atgagtgaga tggagaggct tctgcgttcg gcgctcgtgc cggtcgaccc gccgggaaca
      961 ttggtggacc ggctcgaggc gcgcctgacg gagcagctcg acgcggcggc cgaggagctc
     1021 gccgactggg agcttcgctc gatgcgcaac ccgcgcaact gggtgcgccc ggtggcggcg
     1081 agcgtggcgc tcggcctcgc gggcggcgcg ctggtggtcg tgcgtactcg ccagcagcag
     1141 aagcgccgta gcgcgaccgg tgtgaaggcg atccagaaga gcgtgaacga ggtcgccggc
     1201 gacgtgcgcc ggcggctcaa tcgctagccc gtccgcgcga cgtggctcgg cgccggcgtg
     1261 ggtgaggtga gcgtggccgc gagcaggccg gcgaggccga gcaccaggtg cagcacgtcg
     1321 tccccggtgt tcacggggaa cagcttgaag atcgagtcgc cgtcgccgta gagcgcgccg
     1381 agcagcgcga gcagcaggaa gaacaggccg gccgcgtacg catacgcgcg cgagcccgcg
     1441 taggagcgag cgacgcgaac gccgatcagc ccggcgagaa gatgcagcag gttgtgccag
     1501 ccgtttacgt caaacgcgcc gaacacggcg tcgcggttgg tgagcgtctc gtgtcccgtg
     1561 gcgaagctcg agttgtagaa gaagcccgca atgccgagag ccacgagcag ccagcctgct
     1621 acgcgcgcgt agcgctgggc agacgacttc ggcagctctt ccctcatggc gcgcgggcag
     1681 gttaacgtcg gcgtcggcgc gatgatccaa atcaggcggc gcggcgtaag caggccttga
     1741 tgccgaggac gaaggagctt cagcggctgg cggacgagga gctcatgcag ctcgtccagg
     1801 acggtgatcc gcgcgcgttc gagcttctct acgaccgcca cggcgacgcc gcgttttcac
     1861 tcgcgtaccg catggtcggc gaccgcgtgg ccgcggagga catcacgcag gaggcgttcc
     1921 tctcgatctg gcggagccgc gagcgctacc agcgcgagcg cggcagcgtg cgcacctggg
     1981 tgctcggcat cgtgcatcac cgtgggatcg atgccttgcg gcgcaacctc gtgcatcagc
     2041 gccggcgcgc gagcgcggaa gggatcgagg agagacacga ggcgcccgag cgcaccgacg
     2101 ccgaggcgct ccgccgggcc gaggcgaaga ctgttcacgc cgcgctcgag acgctgcccg
     2161 acgaccagag tcaggtgatc gagctcgcct acttcggcgg cttcacgcat atccagatcg
     2221 cggaaatgct cgcgatgccg gtcggtaccg tgaagggcag gatgagactc ggcctcgaga
     2281 agatgcgcag ggagctcgct ggaggcgtcg catgacgatg gagcagcacg agcgctacgc
     2341 ggagaacgtg gcggcctacc tgctcggcgc cctgaccgag gaggaggcgg ccgagttcga
     2401 ggcgcacctg gcggagtgcg agctctgccg gctcgactat cagcggctgc gcgtcgccgc
     2461 cgaggcgctt ccgcgcgccg tcgagccgct cgaggcgccg gactcgctgc gcgactcgct
     2521 catggacacg gtgcgcgcgg aggcgcgacc cggatcggtg gccgagcgcc gaccgtcgtt
     2581 tcttcagagg cttcgcgacc gcttgggaga ggtctcgccc gcggtggcgg cggtgagcgc
     2641 cgcggccgta ctggcaattg gcgtgctcgg gggctggggc gtgtccgagc tcgcgaacaa
     2701 cggaaccggt gagaagacct acgcggcttc gacgacgttg ccccgcggaa gcgccaccct
     2761 gaccgtgccc gaggaccgca gcggcgcatc gctcacggtc cgcggcatgc cgccacctgg
     2821 tgcgggccgc gtgtacgagg tctggctgag gcgcggctcg acctacgcgc ccgcgggctc
     2881 gctgttcgac gtcggacgca acggcaccgg ctcggcggcc atccccggca acctccgggg
     2941 tgtcgacggc gtggcggtca cgagagagcg ccgcggcggc accctcaagc ccaccgagaa
     3001 gccggtgatg accgtaaatc tcagttaaga cgcctgagcg ccgcactatc gtggtgcgcg
     3061 aaatgggcgt ctgctatcgc catccggacc gggaaacggc cgtttcgtgc tcgaactgcg
     3121 ggcgcgcaat ctgccctgac tgcatgacgc cgacgtccgt gggcatgcgc tgcccggagt
     3181 gttcgcgcga gcgcacgcgg gtgcaccgtg cgcgcacgat gaccgacgag ccgcggctca
     3241 cgtacgcgct ggtcgcgatc aacgtgatcg tgttcctcgc cgagttcttc tccggcgtga
     3301 gcgcgggcgg tggaagcttc ggcggcagcg acctgatccg ccagggcgcg ctctacgggc
     3361 ccgacatagc gaacggcgag gtctaccggc tgctcaccgc cggcttcctg cacgccggct
     3421 tcgcgcacct cttcttcaac atgtactcgc tctacatcct cggctcgctg ctcgagcccg
     3481 cggtcggccg gctgcgcttc gcgatcatct acttcgtgtc gctgttcgcg ggctcgttcg
     3541 gtgccctaat cgcggccccg accacgctga ccgtgggcgc gtccggtgcc atcttcggga
     3601 tgttgggcgc gacactcgtg gtgatgcgct cgcgcggcgt cagcgcgatg gagagcgggc
     3661 tcgggctctg gctgctgctc aatctcggca tcacgttcct cattccgggc atctcgatcg
     3721 gcgggcacat cggcggcctc gtcggcggca cgctggcagc actcgcgata tacgaggcgc
     3781 cgcggctgcg cgtcccgcgc acggccgcgc tggtgctcgc gtcgctcgtc ggggtgatcg
     3841 ctatcgcggg ctcgatcgcc gtcgcctagg tagccgcgca cggtcaaccg cggcggcgcg
     3901 ggcggcggcg cgggggccgc tggccatcat cgtgctttgg cattcgcttg cgccgccgct
     3961 gctcctcggc ggccagctca cggtcctcgc tgagccccac ctcgaagaag acgagcagca
     4021 ccacggcacc cagcccgacc gtgatcagcg tgatgccgag cacctgaccc gttatcccgc
     4081 tgatgaagat gacgcacaac gcgccggcga gcaccaccgc cgcggccgac ccgtatgagg
     4141 tgatgcgggc gcggctgctc atggtccacg cgacccttct agccgccctc gacccgccgc
     4201 ttcaggtcct cgagcgagcc gcgcatcacc tggtcctcca ccatgcgctt cacgggtccg
     4261 ggcacccaca tgccggggtc cagcgccacg cggtaggtgg ccagcgtgct atctccgtcg
     4321 ccctcgagca gcagctcacc gtccacgtcc ttgacgtcgc cctcgacgaa gtcccacgtg
     4381 atgcgatgcg gctcctcgta tgagtaggcg agcgtgtacg acacggtctt caccttcgcg
     4441 tcgatctgga aggccacgtg gtgggcgcgg ccgtcctcgt agcgcgtgac cacctcgcac
     4501 gacttcaccg cgcgctgcca ctccgggaac tcctcgtact cggtgagcgc ggtgaagcag
     4561 tcctccggcg cggcggcgat ctgcatggtc tgctccgctt tcttcgcgcc catggtcagc
     4621 cggctcgcgc gtggatgtcg atctcgatga agcgctccgg cggcggcgac tccacgcggt
     4681 cggggtggag gatgtgcgcc agcagctcga cgccgtccac gagccgcggg ccgggacgcg
     4741 agaaatatgc ggcggcatcg acggcgacga tgcgttccgc cccgagcgac tcgacctcgc
     4801 tgcggtggtc catcacctct tgggccgcgc gctcggcgta gtaaccgcag ggcatcgaca
     4861 cgaccacgcg tggcttggcc gcgcgcagct cgtcccagga aacggtgcgc gacttctcgc
     4921 cgggcaggcc gatcacgtcg acgcctccgg cgagctcgat catctgcggc acccagtggc
     4981 cgccaacgta cggtggagcg agccattcga gcgccgccac aagcaccggc tcggcgccgt
     5041 cgaccgcctc gcgaacgcga tcgatgcgat ccgccgcatc ctccagcagc cagtcgccgg
     5101 cctcgtcgtc ctcggtctcc tccgcgagcc ggcgcacgtc gttcaagacc tcgccgagcg
     5161 tggtcggatc gagcgagagc acacgcggat gggttggaag ctcctccgcg atcgcgcgca
     5221 cgtcgtcgta ggacacggcg cacacctcgc acagcgcctg cgtcacgatc aggtcgggct
     5281 cgagctcgcg cagcttctcc acgtccagct catagatcgc ctcgccgcgc tgcgtgcgct
     5341 cgcgcaccgc gcggtcgatc tcctcggtgg agagcccgtc ggggatcacg ctgcgcgtga
     5401 gatgcggtct cgattccgcc tcgggtgggt agtcacattc gtgggtgacc cccactacgc
     5461 tgtcgcccaa tccgagggcg aagagcatct cggttgccga tggcacaagg cttgcgatcc
     5521 gcacggaact aacgatggca cttctgagcg acgaggacgt acagacccag ctcggcgagc
     5581 ttcgcggttg gagccgcgag ggcgacgcca tcaagaggca gttcacgttc gacgacttca
     5641 agggctcgat cgacttcgtc aaccgcctca cgccggaagc ggagggcatg aaccatcacc
     5701 cagatctcga gatctcttgg aacaaggtca cggtcacgct ctcgactcac tcgcagggcg
     5761 gcctgaccgc caacgacttc gagctcgcac ggcgcatcga ctccgtcgcc tagccgggcg
     5821 cccggaccgc ccgtactgct agcgttgaac acgttcgcct tgatgactga agacgaacct
     5881 cctcgaagta gcgccctcgg gcgtccgtgc cggtggtagc cgtactgctc atcgcagtac
     5941 cggcgctgat cgccgtcaac gcgttcttcg tggccgccga gtacgcgctc gtgcgcgcgc
     6001 ggctcgaccg catcgaggcg ctcgaggagg agggcgcgtc cggcgcgatt ctcgcgcacc
     6061 gccagatcga ccacatcgac gagtacatcg ccgcgtgcca ggtgggcgtc accatggcct
     6121 ccctgggtct gggtgccgtc ggcgagcccg cgttcgcgaa tctgttcgag aactggttcg
     6181 ggggagtggt ctctcacagc gccgccgttg tggtctcggc gatcctcgga tactcggtac
     6241 tgacgacgct gcacatcacc ttcggcgagc tcgtcccgaa gctgtacacg gtcgcgttcg
     6301 ccgagaaggt cgcgcgccgg gtggcccgcc cgctgcaggc ctcgagcgtc atcttcaggc
     6361 cgatctcgag cgtgctgtcg aacgtcgcac gcttggtcct gcgcccgttt ggcatcgagg
     6421 ccgaggggct cggcgaggag cagacgacct cggaggatct caagttcctg atcgcgcgca
     6481 gcgcgagcgg cggggtcctc gaccctggcg aggcgctgat gttgcgcggc gtcttccacc
     6541 tccacgagca ggaggcgcgc caggtgatga ctccgatccc ggccgtggtg acggtcgacg
     6601 tgtcggagac cgtggagact gcgctgcgcc gctgcgtgca gtccgggcac acgcgactgg
     6661 tcgtgaccga ggacgagaac accgaccgag tgcgcgggat cgtgcacaac aactcgctgg
     6721 tgcggctgtt gatgacccgc ggcggcgatg cgtccatcga gcctgcgatc aaggatgcgc
     6781 ccatcgtgcc cgagacgaag ccactcgacg acctgctcgc cgacctccaa cgcgagcgcg
     6841 cgtcgatgtc tgtgatcgtg gacgagtacg gccgcacggc ggggatcgtg accgtcgaag
     6901 acatcatcga agaggtcgtc ggcgagatct cggacgaaac cgaccccgcg ggcggcgccg
     6961 tccgccgcct ggcgaacggc gactggtacg tccgcggcca tgtgccgatc accgacctga
     7021 tcgactacgg catcgagctg ccggtcgaca gcgaggccta caactctgtc ggtgggtacg
     7081 tgttcggcga gctcggacgg ctgccgaaac gcggcgacat ggtggagacg ggcggctact
     7141 cgctacgcgt ggagtccgtg cgcgagaacc gcatcgaggc cgtgcgcatc cgtgatcacg
     7201 agccgcacca gcgcaacggc gacgcgctga aggagtccgt ccgcgaagag ggataggttg
     7261 gcctggcaag agcccgcttc caggaggtct tgccgatgtg gtcagtcagg ctcaccgatt
     7321 cccagcgtga cgccgttcgc gccctgatcg aggcgcaggg ggaggtgggc ccgagcctgg
     7381 gcggtgcatt ggaggcgctc gagttcgcgc gctgggacga cctccccgac gctcgcctcg
     7441 actgggagcg cgtgcagaag ctggcgaatc tgcagggcat cggcgaggcg gacgtggtct
     7501 gggaccttgc ggccgggctg cccaccccgg gcacggcgat cg
//
LOCUS       DASVXS010000298        12231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1270057, whole genome shotgun sequence.
ACCESSION   DASVXS010000298 DASVXS010000000
VERSION     DASVXS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270057"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF752_16675"
     CDS             <1..502
                     /locus_tag="VF752_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8857231.1"
                     /translation="DMEGNGVELYWDRPREVWPHEPDGRLSMYTIPLDVPGLLELPEV
                     EPDPSRVDIGHVHLKVSDIARSLPFYRDLLGLDEQVSWGEAAFLSAGGYHHHVGMNVW
                     ESRGAPPPPEGAAGLGEVEIAVPDAAAVDAVAQRLEAAGVETSREDGRLLFRDPDRIP
                     LAVAPA"
     gene            499..1317
                     /gene="fdhD"
                     /locus_tag="VF752_16680"
     CDS             499..1317
                     /gene="fdhD"
                     /locus_tag="VF752_16680"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX8857232.1"
                     /translation="MSDTGYDPRVARVEIERVRAGRRERAPDELAVEEPLEIRVDGEA
                     LAVTMRTPGDDEDLAAGFLAGEGLIEGPTDIAAIGPTEDFAANIVEVRTRAGLRRDPA
                     GERRFYLTSSCGICGKGALEFVRQALPTDRRDQETAIAPELVLAAPERARAEQHAFER
                     TGGLHATALFESAGELLVLREDVGRHNAMDKAIGSQLLAGRFPLEGAFAQVSGRASFE
                     LVQKAALAGLAGLVAVGPPSTLAVELARERGMLLCGFVRGDSLNLYAGSERLRA"
     gene            1399..2439
                     /locus_tag="VF752_16685"
     CDS             1399..2439
                     /locus_tag="VF752_16685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8857233.1"
                     /translation="MSDQPRDTLEALYRRGEEEGCLDLSEIAAAAEELGIEDTEAIYA
                     EAERRGIEVSDDCGKSEEEVLGRYRLDEVADATSDSLQLFLRDISQRPLLTAAEEVEL
                     AKRIERGDQAAKNRMIESNLRLVVANAKRYRGLGLPFLDLIQEGILGLIRAVEKFDYR
                     RGFKFSTYATWWIRQAMQRGLQHHSRTIRIPVHIGQELTKVRAAERKLASELGREPTA
                     KEIAERVGMDVHQLEELRSAERVPVSLETPVGSEGDAELGSLLPFEGPEPHEEVAVEL
                     EEQSIRKALQRLDANARRVIELRFGIGGNDPLTLREVAKLTGLSAEGVRKLERRALRR
                     LAEERELQALAA"
     gene            2601..3017
                     /locus_tag="VF752_16690"
     CDS             2601..3017
                     /locus_tag="VF752_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857234.1"
                     /translation="MATRATKESTDAYCPHCFLLVERDAHGDWPPSPTRCPHCKLLIG
                     VGRGRSTPAATPGARGTAAGVFAHQAKRGDDAGVSKDAVRAAIRSVAQSLGSRPERLL
                     MVDYQQRASADQSLPQLSEVFAAFGSWKRARREAGA"
     gene            3022..3825
                     /locus_tag="VF752_16695"
     CDS             3022..3825
                     /locus_tag="VF752_16695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX8857235.1"
                     /translation="MSVAGPIEARAYEASNDPELLERYHQAGDIRARQLLIERHMPLV
                     RRLAQRYAQRGEALEDLTQVGCVGLIKAIDRFDGEYQVALASYATPNILGEIKRHFRD
                     KGWAMRVPREVQELNVRLGPVMDQLTVQLGRSPTVDELAAVTRAPRERVVEALESSRA
                     YSALSLSAPSGGDDEGSTAMDALGTDEAGYGRSEDRQLLRQGLETLPERERAILHMRF
                     FQGLTQSEIAARVGISQMHVSRLIRQSVERVRDVLDGPGRSMRRRREPA"
     gene            3865..4362
                     /locus_tag="VF752_16700"
     CDS             3865..4362
                     /locus_tag="VF752_16700"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8857236.1"
                     /translation="MDVAIFIGVWLLVGIGVIFVAFSGGPSRARRAYLTGGRRFFTLL
                     MVLIYVGLGVAVPALIIAGRDEALGDTSKLRGEDAGKLSKGKHLFISTCATCHTLSAA
                     NARGVTGPDLDRIGPVNEARVLGAIKNGGTGQGRMPAGLLEGSNAQAVAAYVSKVAAG
                     QTPGG"
     gene            4530..6680
                     /locus_tag="VF752_16705"
     CDS             4530..6680
                     /locus_tag="VF752_16705"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX8857237.1"
                     /translation="MARTREKNVTAARWSVNGNALGATDLTEPGNQTFSANVFSPVVQ
                     RQALPKHVYRQLQSTVENGEPLDSTLADQVAQAMKEWAMSRGATHYTHWFQPLTGSTA
                     EKHDSFYGPSADGSTAIAEFSGKELIQGEPDASSFPTGGIRATFEARGYTAWDPTSPA
                     FLLENPNGTLLCIPTAFVSWTGEALDNKTPLLRSMDALSRSAVRALRLLGDEAAQRVF
                     TTMGPEQEYFLVDEQYFYERPDLVNCGRTLFGAKPPKGHELDDHYFGSIPERILAYML
                     EVEGELFKLGVPVKTRHNEVAPNQYEIAPVFEGTNVGCDHQMLTMQVLKNVARKYGLV
                     CLLHEKPYAGVNGSGKHNNWSMATDTGVNLLEPGDTPEENLHFLFFCAAVIAGVDKYQ
                     GLLRASVAGTGQDHRLGANEAPPAIMSIFLGSELERVFNGILEGRVDPSEPGAVLGLG
                     TPVLPALPLHSGDRNRTSPFAFTGNKFEFRALGSSMSPSLPNTVLNTIAAASIDELSD
                     KLEAALQGGKELQQAVLDVIRDSYGTSSRVVFNGDGYSDEWHKEAEERGLLNLRTTPD
                     ALPELLSNGSKEAFSRYEVLSDRELESRFEVYAEQYVVNINIEAEIAASIARTMILPA
                     ALRHLALLKDAEVRSLIGEAEGLIEELVAAITDLETANREHPEEEGLGHARYMRDRVI
                     PAMERTRAVADQLERVVADDLWPLPKYQEMLFIK"
     regulatory      7016..7170
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            7173..7562
                     /locus_tag="VF752_16710"
     CDS             7173..7562
                     /locus_tag="VF752_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857238.1"
                     /translation="MSQAEPRRASGRSAWNWDAFPSETRRSARSGVERPVRRASGPAR
                     PKGADVVPIRPRAEAGRRTVRITGQASTPPRRSRPAVVRVGSRPDRFALWAVGLGLFM
                     ALLAAATAHGAEPTAAHHAAQLLAHTR"
     gene            7743..8306
                     /locus_tag="VF752_16715"
     CDS             7743..8306
                     /locus_tag="VF752_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857239.1"
                     /translation="MKTKESRFQVHDELTAPEGSLPVLKGALATGGQLSNFLGVLAGS
                     PATLRAYARFRSELRHGVLPWPTQQRIAIAVAEHQNSEYAMNLLQRSARDAGIGLDEF
                     ALARRFESQDEREATLLHFVKALLESPSAPPLHFVEEAREAGWTDEQLLETVAHVALA
                     SFGNLVTRAGDVPRDGSAEEGRLVQAA"
     gene            8327..8701
                     /locus_tag="VF752_16720"
     CDS             8327..8701
                     /locus_tag="VF752_16720"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8857240.1"
                     /translation="MAAPAATAAKAARQGSTKQGPQGRSECCTLYHQAVEMVGKRWTG
                     AILMVLLDGPLRFSEVRSLVPDLSDRLLSERLKELEAEGIVARRVLEGTPVRVEYALS
                     EKGRALEPAVRALKAWANAWLD"
     gene            8782..10140
                     /gene="glnA"
                     /locus_tag="VF752_16725"
     CDS             8782..10140
                     /gene="glnA"
                     /locus_tag="VF752_16725"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX8857241.1"
                     /translation="MAVQQQQPKTADDVIAMVEDHDIRFIRFWFTDILGQLKSFSINK
                     DELEDAFEGGMGFDGSSITGFNAIEESDMIAMPDASTFAILPWRPADKGVGRMFADIV
                     TPAREPYEGDPRHVLRRALQRAESMGFDTFNVGPELEYFYFRNAKPENGQPEILDEGG
                     YFDLTTLDAGSDVRRETVLALEQLGIHVEYTHHEVGPSQHEIDMRYAGAMKMADDCMT
                     YRIVVKEYAMKYGWHATFMPKPLFGENGSGMHTHQSLFKNGQNAFYDADDQYFLSDVG
                     KAFIAGQLRHAREISSIFAQWVNSYKRLVPGFEAPVYCAWSRRNRSALVRVPLYHPGK
                     EQATRMELRCPDPACNPYLTFAVLLMAGLEGIEKGYELPEPMEKNLYHLSPDERKRLG
                     IAQLPETLGEAIELTAESELVLRTLGEHMFNRYVEIKRKEWEEYRIQVSRWELDRYLS
                     IL"
     gene            10204..10815
                     /locus_tag="VF752_16730"
     CDS             10204..10815
                     /locus_tag="VF752_16730"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8857242.1"
                     /translation="MGEGGPRDTGWRALFDAAFRNSRNAMLLVDDRRRIVEANGAFLR
                     LLGYSLDELIGRQIWDFVVGGPLATDEEWAAALEEQRFTGHADLRTAGGETVSVQYGA
                     HVETVTGQRLVLFVVLSSSKAGGAFRREPAAAGAAPGELTLREIDVVRLTADGMTAPE
                     IADDLGISNHTVRTHLRNAMEKLGARSRAHLVAISLGAGHALA"
     gene            10832..11053
                     /locus_tag="VF752_16735"
     CDS             10832..11053
                     /locus_tag="VF752_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857243.1"
                     /translation="MVSTLGVALATIESMLSVDQGSPFCALVCSICHEQIKPGDLVLA
                     AYHRGTPKAIHAHVCRSAQAEKPVEPTRK"
     gene            complement(11059..>12231)
                     /locus_tag="VF752_16740"
     CDS             complement(11059..>12231)
                     /locus_tag="VF752_16740"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX8857244.1"
                     /translation="YDNALLARAYLHGWLVTEDPVFRRVTTETLNWALREMRGLEGGF
                     YSALDADSEGEEGKFYLWSLEELRDAAGDEADVAEAYFGATEEGNFEGRNILVRNDPD
                     PDDVADLKQRLYDVRAKRVWPGLDDKRLTSWNALMIGALAEAGAVLEREDYLDAARGA
                     ADFILRDLRSDDGRLLRTWKDGRGKLNAYLEDHAFLLEALLSLYEATFEPRWFAAARE
                     TADAMIARFADDDNGGFFETSSDHERLVARRKDLEDNPIPSGNSAAAYGLLRLAALTG
                     ENQYAARAEGVLRLLHELAPRHPQAFGHLLQALDFYFAQVKEVALVGPGARPLERVVR
                     SAFRPHLVLAGGEEDGVPLLEGRSPVDGRAAAYVCENFACQRPVTEPADLEALLGS"
BASE COUNT         1995 a         4186 c         4258 g         1792 t
ORIGIN      
        1 cgacatggag gggaacggcg tcgagctgta ctgggaccgc ccgcgcgagg tgtggccaca
       61 cgagcccgac ggccggctca gcatgtacac gatcccgctc gacgtgccgg gcctgctcga
      121 gctgcccgag gtcgagccgg atccctcgcg cgtggacatc ggccacgtgc acctgaaggt
      181 gtcggatatc gctcgctcgc tgcccttcta ccgcgacctg ctcggcctcg acgagcaggt
      241 gagctgggga gaggccgcgt tcctgtcggc gggcgggtat caccatcacg tgggcatgaa
      301 cgtgtgggag tcgcggggcg cgcctccgcc gcccgagggc gcggccgggc tcggcgaggt
      361 ggagatcgca gtgccggacg ctgccgccgt ggacgcggtg gcgcagaggc tcgaggcggc
      421 tggtgtcgag acgagccgcg aggacggccg cctgctcttc cgcgatccgg accgcatccc
      481 cctggcggtg gcgccggcgt gagcgacacc gggtacgacc ccagggtcgc ccgggtcgag
      541 atcgagcgcg tgcgcgcggg gcggcgcgag cgcgcaccgg acgagctggc tgtggaggaa
      601 ccgctcgaga tccgcgtgga cggcgaggcg ctcgcggtga cgatgcgcac acccggggac
      661 gacgaggacc tggcggccgg gttcctggca ggcgagggtc tgatcgaggg tcccacggac
      721 atcgcggcaa tcggcccgac cgaggacttc gcggcgaaca tcgtggaggt gcgcacgcgc
      781 gcgggtctcc gtcgcgaccc cgccggcgag cgccgcttct acctgacttc gtcctgcggc
      841 atctgcggga agggagcgct cgagttcgtg cgccaggctc tgccgactga ccgccgcgac
      901 caggagacgg cgatcgcgcc ggagctcgta ctcgcggcgc ccgagcgcgc tcgcgcggag
      961 cagcatgcat tcgagcgcac cggtggcctc cacgcgacag cactcttcga gtccgccggc
     1021 gagctgctcg tgctgcgcga ggatgtcggc cgccacaacg cgatggacaa ggccatcggc
     1081 tcgcagctgc tcgccggtcg cttcccgctc gagggcgcgt ttgcccaggt aagcggccgc
     1141 gcctcgttcg agctggtgca gaaggccgcg ctcgccgggc tggccggcct ggtcgcagtg
     1201 gggccgcctt cgaccctcgc ggtcgagctg gcgcgcgagc gcggaatgct gctgtgcggc
     1261 ttcgtccgcg gcgattcgct gaacctttat gcaggttccg agcgcctgcg cgcctgaacc
     1321 tccaacaagc gccgaaaggg aggggaacgg cgccgcgcag cgtattgccg gagtcgtatc
     1381 ctgaggctga gcaacccgat gagcgaccaa ccgcgcgaca cactcgaggc gctttaccgc
     1441 cgaggcgagg aggagggatg cctcgatctg tcggagatcg ccgcggccgc cgaggagctc
     1501 gggatcgagg acaccgaggc gatctacgcg gaggccgagc ggcgcgggat cgaggtctcc
     1561 gacgactgcg gcaagtcgga ggaggaggtg ctcggtcgct accggctcga cgaggtcgcc
     1621 gacgcgacga gcgactccct ccagctcttc ctgcgcgaca tctcacagcg tcccctgctc
     1681 acggccgccg aggaagtcga gctcgcgaag cggatcgagc gcggcgacca ggccgccaag
     1741 aaccgcatga tcgagtcgaa cctgcgcctc gtggtggcga acgccaagcg ctaccgcggc
     1801 ctcgggctgc cgttcctcga cctgatccag gagggcatcc tcgggctcat tcgcgccgtc
     1861 gagaagttcg actaccggcg cggattcaag ttctccacct acgcgacctg gtggatccgc
     1921 caggcgatgc agcgcggcct ccagcaccac tcgcgcacca tccgcatccc ggtccacatt
     1981 gggcaggagc tgaccaaggt ccgggctgcc gagcgcaagc tcgcgagcga gctcggtcgc
     2041 gagcccaccg ccaaggagat cgccgagcgc gtgggcatgg acgtgcacca gctcgaggaa
     2101 ctgcgctccg ccgagcgcgt ccccgtcagc ctcgagacac cggtcggctc cgagggcgac
     2161 gccgagctcg gctcgctgct gccgttcgag ggtccggagc cgcacgaaga ggtggcggtc
     2221 gagctcgagg agcagtccat ccggaaggcg ctgcagcggc tcgacgccaa cgctcgccgg
     2281 gtgatcgagc tgcgattcgg catcggcggc aacgacccgc tgacgctgcg cgaggtcgcg
     2341 aagctcaccg gcctctccgc cgaaggcgtg cgcaagctgg agcgccgggc gctgcggcgc
     2401 ctcgccgagg agcgcgagct ccaggcgctc gctgcgtgaa ccgcagcgcg cctcttctcc
     2461 gtacaccagt ctgagcggac cggcccgctg ccggggcgag agttgagctt ggactaaagc
     2521 ctcgctacca ggcgtttaca gcttgctaaa tgcggcgaac acagagtagg gaatcacaca
     2581 aggacaggga cggaggagag atggccacga gggcgacaaa ggagagcact gacgcctact
     2641 gccctcactg ctttctgctc gtggagcgcg atgcgcacgg tgactggccg ccgtctccca
     2701 cgcgctgccc ccactgcaag ctgctgatcg gcgtgggacg cggcaggtcc actccggcgg
     2761 ccacgccagg cgcccgtggc acggctgcgg gagtgttcgc gcaccaggcg aagcgcggcg
     2821 acgatgccgg ggtctcgaag gatgcggtgc gggccgcgat ccgctcggtc gcgcagtcgc
     2881 tcggctcgag gccggagcga ctgctgatgg tggactacca gcagcgcgcg tcggccgatc
     2941 agagcctccc gcagctgagc gaggtcttcg ccgccttcgg cagctggaag cgagcccggc
     3001 gagaggcggg cgcctgagga gatgagtgtc gccgggccca tcgaagcccg ggcctacgag
     3061 gcctccaacg accctgagct gctcgagcgc taccaccagg caggcgacat tcgtgcccgc
     3121 cagctcctga tcgagcggca catgccgctc gtgcggcggc tggctcagag gtacgcgcag
     3181 cggggtgagg cgctcgagga cctcacgcag gtcggctgcg tgggactgat caaggcgatc
     3241 gaccgcttcg acggcgaata ccaagtagcg ctggcgagct acgcgacgcc caacatcctc
     3301 ggcgagatca agcgccactt ccgcgacaag ggctgggcga tgcgcgtgcc gcgcgaggtg
     3361 caggagctga acgtccggct cggcccggtc atggaccagc tcaccgttca gctcgggcgt
     3421 tcgccgaccg tcgacgaact ggccgcggtc acgcgagcgc cgcgtgagcg cgtcgtcgag
     3481 gcgctcgagt cgtcgcgtgc gtattccgcg ctctcgctct cggcgccctc gggcggcgac
     3541 gacgagggca gcacggcgat ggacgccctc ggcacggacg aggccggcta cgggcgctcg
     3601 gaggaccggc agctgctgcg ccagggcctc gagacgcttc ccgagcgcga gcgcgcgatt
     3661 ctccacatgc gcttcttcca aggcttgacg cagtccgaga tagccgcccg cgtcggcatc
     3721 tcgcagatgc acgtttcgcg cctcatccgg cagtccgtcg agagggtgcg cgacgtgctc
     3781 gacggaccgg gacgctcgat gcgccgccgt cgcgagcccg cctgagcggc ggacgtcgcc
     3841 ccgcttgatt acgattcccc gcccgtggac gtagcgatct tcatcggcgt ctggctgctg
     3901 gttggaatcg gcgtcatctt cgtcgccttc tccggcgggc cgagccgcgc gcgccgggcg
     3961 tatctcaccg gcgggcggcg cttcttcacc ctgctgatgg tgctgatcta cgtggggctc
     4021 ggagtggccg tgccggcgct gatcatcgcc ggccgggacg aggctctcgg ggacacctcc
     4081 aaactccgcg gcgaggacgc tggcaagctc agcaagggca agcatctctt catcagtacc
     4141 tgcgcgacct gccacacgct ctcggccgcg aacgcgcgcg gtgtcaccgg gccggatctc
     4201 gaccgcatcg gaccggtgaa cgaggcgcgc gtactcgggg cgatcaagaa cgggggcacc
     4261 ggccagggac ggatgccggc cggcctcctc gagggatcca acgcccaggc ggtggccgcg
     4321 tacgtctcga aggtcgcggc gggccaaacg cccggcggct gacgctgtct gtacgcgtgg
     4381 agaaagacag gggcggaatt tcctacacgc ggacactgcc agccggatga gccccgcgta
     4441 gattccccgg agccggccgg tgcgagccac gcatgcccgc gcgccgccgc gtccgctcga
     4501 aacccgtgac agagacagga gcagcagact tggccaggac tcgagagaag aacgtaaccg
     4561 cggcccgctg gagcgttaac ggcaacgccc tcggcgccac agacctcacc gagccgggga
     4621 accagacctt ctccgccaac gtcttcagcc cggtggtgca gcgccaggcg ctgccgaagc
     4681 acgtgtaccg gcagctccag tccaccgtgg agaacgggga gccgctcgac agcacgctcg
     4741 cggaccaggt cgcccaggcg atgaaggaat gggccatgag ccgcggcgcg acccactaca
     4801 cgcactggtt ccagccgctc acgggctcga ccgccgagaa gcacgactct ttctacggac
     4861 cctcggccga cggcagcacc gcgatcgccg agttctccgg caaggagctg atccagggcg
     4921 agcccgacgc ctcgagcttc ccgaccggtg gcatccgcgc gaccttcgag gcccgcggct
     4981 acaccgcctg ggacccgacc tcgccggcgt tcctgctcga gaacccgaac ggcacgctgc
     5041 tctgtatccc gaccgcgttc gtgtcgtgga cgggcgaggc gctcgacaac aagaccccgc
     5101 tgctgcgctc gatggacgcg ctctcgcgct ccgctgtgcg ggccctgcgc ctgctcggcg
     5161 acgaggccgc tcagcgcgtc ttcacgacga tgggcccgga gcaggagtac ttcctggtcg
     5221 acgagcagta cttctacgag cggcccgacc tcgtgaactg cggccgcacg ctgttcggcg
     5281 ccaagccgcc gaagggccac gagctcgacg accactactt cggctcgatc cccgagcgga
     5341 tcctcgccta catgctcgag gtcgagggcg agctgttcaa gctcggcgtg ccggtgaaga
     5401 cgcgccacaa cgaggtggcg ccgaaccagt acgagattgc gccggtgttc gagggcacga
     5461 acgtgggctg cgaccaccag atgctcacca tgcaggtgct caagaacgtg gctcgcaagt
     5521 acggcctggt gtgcctgctg cacgagaagc catacgcggg cgtgaacggc tcgggcaagc
     5581 acaacaactg gtcgatggcc acggacaccg gcgtcaacct gctcgagccg ggcgacacgc
     5641 ccgaggagaa cctgcacttc ctgttcttct gcgcggcggt gatcgccggc gtggacaagt
     5701 accaggggct gctgcgcgcg tcggtggccg gcacggggca ggaccaccgc cttggcgcga
     5761 acgaggcgcc gcccgcgatc atgtcgatct tcctgggcag cgagctcgag cgcgtgttca
     5821 acgggatcct cgagggtcgg gtcgacccgt ccgagccggg cgcggtgctc ggcctcggca
     5881 cgccggtgct gcccgcactg ccgctccaca gtggtgaccg gaaccgtacg agcccgttcg
     5941 cattcaccgg caacaagttc gagttccgcg cgctcggctc gagcatgtcg ccgagcctgc
     6001 ccaacacggt gctgaacacg atcgccgcgg cgtccatcga cgagctgtcg gacaagctcg
     6061 aggccgccct ccagggcggc aaggagctcc agcaggcggt gctcgacgtc atccgcgact
     6121 cgtacggcac cagcagccgc gtggtgttca acggcgacgg ctactcggac gagtggcaca
     6181 aggaggcgga ggagcgcggc ctgctgaacc tccggaccac gccggacgcg ctgccggagc
     6241 tcctgagcaa cggctcgaag gaggcgttct cgcgctacga ggtgctctcg gaccgcgagc
     6301 tcgagtcgcg cttcgaggtc tacgccgagc agtacgtggt gaacatcaac atcgaggcgg
     6361 agatcgctgc gtcgatcgcc cgcacgatga tcctgccggc ggcgctgcgc cacctcgcac
     6421 tcttgaagga cgccgaggtg cgctcgctca tcggcgaggc ggaaggcctc atcgaggaat
     6481 tggttgcggc gatcacggat ctcgagacag cgaaccgaga gcacccagaa gaggagggtc
     6541 tcggacacgc caggtacatg cgcgaccggg tcatacctgc gatggagcgg acacgcgccg
     6601 tcgcggacca gctcgagcgc gtggtggcag acgacctatg gccgctgccg aagtaccagg
     6661 agatgctctt catcaagtga tctcagacgg ggcgggccgc acggcccgcc ccgtcgcagc
     6721 ggtccaccgc gcctgtgccg gctgcaagtc ctgacttagt tgccggatag cggcaagaaa
     6781 ctcagcagtt gcgttccccg cgctcgcatc gcgaatgccg gctccaagtc ctgactttgg
     6841 ttccgcatat gagaaccaaa ctcaggactt acggcacttg gcgcccgttc gggccagcag
     6901 ggcccgtggc gcccgggctg gccgcgcggc ccgcccgttt tccttcctct gcaaggtttt
     6961 ttgcgcggtt tgcggggtca ttggaacggc gtgtccgccg cctttgctac gttgaggccc
     7021 gtcaatgcct aagcccccct tggtccgggg gcgcggggga accaaagttg cggccgtgag
     7081 gccgcggggc gaatcgccgg ttttccggcg tagcgctgct ctttctgcgc gagcccgtca
     7141 gctaaccccg taggcccgga gaaagagaga cgatgagcca agccgagcca cgcagggctt
     7201 cggggcgaag cgcctggaac tgggacgcat tcccgagcga gactcgccgc tcggcgcgca
     7261 gtggcgttga gcgcccggtt cgccgcgcca gcggtccggc acgtccaaag ggcgccgacg
     7321 tcgtgcccat ccggccccgc gcggaggccg gcaggcgcac cgtccgcatc acggggcagg
     7381 cgagcacacc gccgcgccgc agccgcccgg ctgtggtccg cgtgggttcc cggcccgacc
     7441 gctttgcgct ctgggccgtt ggcctcggcc tcttcatggc gctgcttgcg gcggcgaccg
     7501 ctcacggcgc cgagcccact gcggcgcacc acgcggccca gctgctggcg cacacgcgct
     7561 gacgaatcgt caggcgttcg ggcgcgagct tcacaaggtt tccgaaagga aatcgctcaa
     7621 cgcgaacttt ctgtgtttgt gcgccgtctt tagaggtaga gcgcagatag caggggaggg
     7681 ccttgtcggt ctacaatcgt gacttaccta gcgtaaggaa cacgcagaaa gtgaagtagc
     7741 ggatgaagac caaggagagc aggttccagg tccacgacga gctcaccgcc cctgagggca
     7801 gccttccggt gttgaagggc gctctcgcga ccggggggca gctgtccaac ttcctcggcg
     7861 tgctcgccgg ctcgccggcc acactccgcg cgtacgcacg cttccgttcg gagcttcgcc
     7921 atggcgtgct gccgtggccg actcagcagc ggatcgcgat cgcggtagcc gagcaccaga
     7981 acagcgaata cgccatgaac ctgctccaac gcagcgctcg cgacgcgggc atcggcctcg
     8041 acgaattcgc gctggcgcgg cgcttcgagt cgcaggacga gcgcgaggcc acgctgctgc
     8101 acttcgtgaa ggcgctgctc gagagccctt cggcgccgcc gctccacttc gtggaggagg
     8161 cgcgcgaggc gggatggacc gacgagcagc tgctcgagac ggtggcccac gtcgcgctcg
     8221 cgagcttcgg caacctcgtc acccgcgcgg gggacgtgcc ccgtgacgga tcagcggaag
     8281 agggcaggct cgtccaagcc gcctgattcc tgtctacgat tcagtcatgg ccgccccagc
     8341 ggcaacggcg gcgaaagccg cccgacaagg gagcacaaag cagggtccgc agggtcggag
     8401 cgaatgctgc accctctacc accaggccgt ggagatggtg ggcaagcgct ggacgggcgc
     8461 gatcctcatg gtgctgctcg acggcccgct gcgcttctcg gaggtccgca gcctcgtgcc
     8521 cgatctgtcg gaccgcctgc tgtccgagcg cctcaaggag ctcgaggccg agggcatcgt
     8581 cgcccgccgc gtgctcgagg gcacgccggt cagggtggaa tacgccctga gcgagaaggg
     8641 gcgcgcgctc gagcccgctg tccgcgccct caaggcgtgg gccaacgcct ggctggatta
     8701 ggcgccgcga acctgcggta actgcgctga gggattagcc tacggcgctc ctcgctacgt
     8761 cgcgcacccg agaggagccg tatggccgtc cagcagcaac aaccgaagac cgccgacgac
     8821 gtcatcgcga tggtcgagga ccacgacatc cggttcatcc ggttctggtt cacggacatc
     8881 ctcggacagc tcaaaagctt ctccatcaac aaggacgagc tggaggacgc tttcgaaggc
     8941 ggcatgggct tcgacggctc ttcgatcacc ggcttcaacg cgatagagga gtcggacatg
     9001 atcgcgatgc ccgacgcctc cacgttcgcg atcctcccgt ggcgcccggc ggacaagggc
     9061 gtcggccgca tgttcgcgga catcgtcacg ccggcgcgcg agccgtacga gggcgatccg
     9121 cgtcacgtgc tgcggcgagc gctccagcgg gcggagtcga tgggcttcga caccttcaac
     9181 gtcggccccg agctcgagta cttctacttc aggaacgcga agccggagaa cggccagcct
     9241 gagatcctcg acgagggcgg ctacttcgac ctgaccacgc tcgacgcagg ctctgacgtt
     9301 cggcgcgaga ccgtgctcgc cctcgagcag ctcggcatcc acgtggagta cacgcaccac
     9361 gaggtcgggc cctcgcagca cgagatcgac atgcgctacg ccggcgcgat gaagatggcg
     9421 gacgactgca tgacgtaccg catcgtcgtg aaggagtacg cgatgaagta cggctggcat
     9481 gcgaccttca tgccgaagcc gctcttcggc gagaacggct cgggcatgca cacacaccag
     9541 tcgctcttca agaacgggca gaacgcgttc tacgacgccg acgatcagta cttcctctcg
     9601 gacgtcggca aggcgttcat cgccggacag ctgcgccatg cgcgcgagat ctcgtcgatc
     9661 ttcgcccagt gggtgaactc ctacaagcgg ctcgtgccag gcttcgaggc gccggtgtac
     9721 tgcgcctggt cgcgccggaa ccgctcggcg ctcgtgcgcg tgcccctgta ccacccgggc
     9781 aaggagcagg ccacgcgcat ggagctgcgc tgcccggacc cggcgtgcaa tccgtacctg
     9841 acgttcgcag tgctgctgat ggcggggctc gagggcatcg agaagggcta cgagctgccc
     9901 gagccgatgg agaagaacct ctaccacctg tcgcccgacg agcggaagcg gttagggatt
     9961 gcacagctgc ccgagacgct cggcgaggcg atcgagctga cagccgagtc cgagctcgtg
    10021 ctgcggacac tcggcgagca catgttcaac cgctacgtcg agatcaagcg caaggagtgg
    10081 gaggagtacc ggatccaggt gtcgcgctgg gagcttgacc gctacctgtc gatcctctga
    10141 cccagcgcca catcacccgt agcgggtgag gatccgctga gtagcggtgc ttaggctcgc
    10201 tgggtgggtg agggcggacc acgcgacacg ggctggcgtg cgctgttcga cgcggctttc
    10261 cggaactcgc gcaacgcgat gctgctggtc gacgaccgcc gccgcattgt ggaagccaac
    10321 ggcgctttcc tgaggctgct cggttactcg cttgacgagc tgatcggacg ccagatatgg
    10381 gacttcgtcg tcggcggtcc gctcgcgaca gacgaggaat gggcggccgc gctcgaggag
    10441 cagcgcttca ccggccacgc cgatctgcgc accgcgggcg gcgagacggt ctccgtccag
    10501 tacggggcgc acgtcgagac cgtgacgggc cagcggctgg tgctgttcgt ggtgctcagc
    10561 agctccaagg caggaggagc ttttcggcgc gagccggccg cggccggcgc cgcgcccggt
    10621 gagctcacgc tgcgcgagat cgacgtggtg cggctcacgg cggacggcat gaccgcgccc
    10681 gagatcgccg acgatctcgg catctctaat cacaccgtgc gtactcacct gcgcaacgcg
    10741 atggagaagc taggtgcccg ctcccgcgcc catctggtcg caatctcgct gggcgcagga
    10801 cacgcactgg cgtgagcagc ctcgcacaac ggtggtatcg acgctcggcg tcgccctcgc
    10861 caccatcgag tcgatgttgt ccgtcgacca aggaagccct ttctgcgccc tcgtgtgctc
    10921 gatctgccac gagcagatca agcccggcga cctcgtgctg gccgcgtatc accgcgggac
    10981 gccgaaggcg atccacgcgc acgtctgccg ctcggctcag gcggaaaagc ctgttgagcc
    11041 gacccgcaag tagctgtctc aggaaccgag cagagcctcg agatcagccg gctcggtgac
    11101 cggccgctgg caggcgaagt tctcacacac gtacgcggcc gctcgcccgt cgacgggtga
    11161 gcgaccctcg agaagcggca caccgtcctc ctcgccgccc gccagcacca ggtgcggccg
    11221 gaaggccgag cgcacgaccc gctcgagcgg gcgcgctccg ggtcccacca gcgcgacctc
    11281 cttcacctgc gcgaagtaga agtcgagcgc ctgcagcagg tgcccgaacg cctggggatg
    11341 gcgcggcgcg agctcgtgca gcagcctcag cacgccttcc gcgcgagcgg cgtactggtt
    11401 ctcgccggtc agcgcggcca gccgcagcag cccgtaggcc gccgccgagt tgccagacgg
    11461 gatcgggttg tcctcgaggt ccttgcgccg tgccacgagc cgctcgtggt ccgacgaggt
    11521 ctcgaagaag ccgccgttgt cgtcgtcggc gaagcgggcg atcatcgcgt cggctgtctc
    11581 gcgcgcggcg gcgaaccagc gcggctcgaa ggtcgcctcg taaagcgaga gaagcgcctc
    11641 gagcaggaaa gcgtggtcct cgaggtaggc gttgagcttg ccgcggccgt ccttccacgt
    11701 gcggagaagg cgcccgtcgt cgcttcgaag gtcgcgcagg atgaagtcgg cggcgccgcg
    11761 agcggcgtcg aggtagtcct cgcgctcgag caccgcgccc gcctccgcta gcgcaccgat
    11821 catgagcgcg ttccacgagg tgagccgctt gtcgtcgaga cccggccaca cgcgcttcgc
    11881 gcgcacgtcg tagaggcgct gcttcaggtc cgcgacgtcg tccggatccg ggtcgttgcg
    11941 cacgaggatg ttgcggccct cgaagttgcc ctcctcggtg gcgccgaagt aggcctccgc
    12001 cacatcggcc tcgtcgcccg ccgcgtcgcg cagttcctcg agcgaccaga ggtagaactt
    12061 gccctcctcg ccctcggagt cggcgtcgag cgccgagtag aacccgccct caagcccgcg
    12121 catctcgcga agcgcccagt tgagcgtctc ggtggtgacg cgacggaaga ccgggtcctc
    12181 ggtcacgagc cagccgtgca ggtacgcgcg cgcgagcagc gcgttgtcgt a
//
LOCUS       DASVXS010000299        18233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_263569, whole genome shotgun sequence.
ACCESSION   DASVXS010000299 DASVXS010000000
VERSION     DASVXS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 18233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18233
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263569"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /gene="tgt"
                     /locus_tag="VF752_16745"
     CDS             complement(<1..941)
                     /gene="tgt"
                     /locus_tag="VF752_16745"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX8857245.1"
                     /translation="MPQPTFEINARAGRARAGVLHLEHGRLRTPAFVPLASTATVKGL
                     SSAEVAALGYEMVLGNTFHLFIQPGQEHIAKLGGLHEFMNWKRPIITDSGGFQVFSMG
                     HGSIAEEIKRRRPGQSRILSIEEEGVRFQSYLDGSEKFMGPETSMEAQAALGSDVALA
                     FDECTPFHVDRDYTARSTERTHRWLDRCIDWHEQYAPEWQLFYGIIQGGVYEDLRQQS
                     AERVASARVDGIAVGGSLGQEKGEMHEVVQWALRPTPEHLPRHLLGIGDVDDIVHAVE
                     AGIDTFDCATPTRLGRHGTALVQDPDARWRMDLHKPH"
     gene            complement(959..1426)
                     /locus_tag="VF752_16750"
     CDS             complement(959..1426)
                     /locus_tag="VF752_16750"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX8857246.1"
                     /translation="MALLVRPEPFSTQDFDRLFNTLLEPFERGRVQRWSPPMDLVEAE
                     DSFILKADLPGMSEDDVAIEVHDGTLRLSGERHAEHEQSEKGWYRVERSFGKFSRSMT
                     LPDGVDPDKIQAKFDRGVLEVRIPKPEERKPRRISIANDPGKPETLEGAASDR"
     gene            complement(1745..2563)
                     /locus_tag="VF752_16755"
     CDS             complement(1745..2563)
                     /locus_tag="VF752_16755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8857247.1"
                     /translation="MAADGLFGPGSITWRVNRENVLVLGGGRALILQVAHPLIAAGVA
                     AHSQYRDDPWGRLNRTLDLATALVFGSEERARAVARHLWHVHGRVNGATHEGCARFPA
                     GSRYSARDPELLMWVHATLVDTALLVYDRHVGGLSERDRRRYYDEQRALAELLGVPLD
                     RQPPHLRAFRAYIAEMLEGDSLAVTSTLRDVVAATLRPPVPLAARPFAEAFALVTVAM
                     LPERLRDELGLPFGPARAGLYEASRFALRTALPLLPAVVREAPPARRAAKREAT"
     gene            complement(2623..3102)
                     /locus_tag="VF752_16760"
     CDS             complement(2623..3102)
                     /locus_tag="VF752_16760"
                     /inference="COORDINATES: protein motif:HMM:NF018098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allene oxide cyclase family protein"
                     /protein_id="HEX8857248.1"
                     /translation="MKRKLSVAIALAAIVLVVLAVAAWGKGKSHSRGDSRGGKVVTVI
                     EHQTGVATTDTGAPGDSPGDLLTFANDVFNAKDTAKVGTDQGYCVRTVAGTAFECNQT
                     TFLPKGQITVEGPFYDAKNSTLAITGGTGRYRNARGFMELKSMAGGAKFKLVFHIIG"
     gene            complement(3164..4003)
                     /gene="fabI"
                     /locus_tag="VF752_16765"
     CDS             complement(3164..4003)
                     /gene="fabI"
                     /locus_tag="VF752_16765"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF005908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX8857249.1"
                     /translation="MTLEGKRILITGVMTKESIGYAVAEEAQRAGAEVILTSFGRAMR
                     LTERVARRLQGEPEVLELDVNEPEHFERLRQELQERWGGVDGALHAIAFAPEDALGGN
                     FMRTPSESAVTAFLTSAFSLKALGESLDPLFQESGGGSLVGLDFDASRAWPIYDWMGV
                     SKAALESVARYLARDLGPHGVRVNLVSAGPLETTAARGIPGFPELAEAWQAQAPLGWD
                     VDDRTPVAAATCFLLSDWSRGITGEILHVDGGYHAMGSRLDGRGASAEAEEHENEKEP
                     ANV"
     gene            complement(4000..4500)
                     /locus_tag="VF752_16770"
     CDS             complement(4000..4500)
                     /locus_tag="VF752_16770"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX8857250.1"
                     /translation="MSNLFTRNFDSLVDGESFVTRGRTITETDVVSFATLTGDFHPQH
                     MDAAWAGESRFGNRIAHGMLVLSYAVGLVPLDPDRVVALRRVRDVVFKQPVHIGDTIH
                     VEGRLGRREPIDEEHGTVTTAWKVLNQRGRTVLRAEVEAIWRRAPATQETDGEVNPAA
                     PEVVPL"
     gene            complement(4497..5090)
                     /locus_tag="VF752_16775"
     CDS             complement(4497..5090)
                     /locus_tag="VF752_16775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8857251.1"
                     /translation="MAIGDSFTGGAPGQLDETRFPDELARELAVADYHNLGVAGATTP
                     QVAGTQLEPCIELRPDLVTVICGANDVLLSVRPDIDVHAAALDEMLQRLLAEVPDAIV
                     VTGTTPDLANFLPLRPRSRQRVTEGMQRLNDATRMVADRYGVLCLEFAEHPDAAERGH
                     FADDGYHPSPEGSRQAARGAALELEKHYGIRRAEEGT"
     gene            complement(5125..6582)
                     /locus_tag="VF752_16780"
     CDS             complement(5125..6582)
                     /locus_tag="VF752_16780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX8857252.1"
                     /translation="MTSQRETTLERAPERLSAGERLELLCDASSLRVIRSRVSSPRMG
                     SKARPGDGVIGASGEVDGRPVYCYAQDASFAGGSLGQAHADTIVRVMRLAGEARVPVV
                     GFIESGGARMQEGTAALGGYGRIFRENVRLSGRVPQISVIGGTSAGGGSYSPALTDFV
                     VMTERASMFLTGPAVVRDVLGEEVSGAELGGARVHERNGVAQFVVPGDADSIFLAREL
                     LSFLPQNAWESPPRSAPIDAPGDDPAGFVPAEERSVYDVRDVIRAIADGGELLEAAQK
                     WARNVVTAFARIEGRPVGVIANQPKHLGGTLDADSAQKAARFVRACNNYGLPLVVLVD
                     APGFLPGTKQERAGVIRHGAKLLHAFAEARVPKLTVVLRKAYGGAYITMNSKDLGADF
                     AYAWPDAEIGVMGPTQAVGIIRRREIEAADDPVSARDAYAAEYADEHLNAEIAAREGF
                     IDEVIEPAETRERLASNLATLPKDRGLDRAGNIPL"
     gene            complement(6582..7784)
                     /locus_tag="VF752_16785"
     CDS             complement(6582..7784)
                     /locus_tag="VF752_16785"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX8857253.1"
                     /translation="MSTRVVITGAGAVTPLGVGATTLLERWAGGECGIEDGEGRCSDF
                     EPADVLSKKELRRTDRFVQFAIAACAEAFEQAGWSDEVPVEPDRVGCIVGTGIGGLSS
                     LEDQHDVLRERGAEKLSPLAVPLMMANAGSAGLAMRHGLGGPAFSTVSACAAGADAIG
                     TASRLIRTGDADAMVAGGAEAAITPLATTAFAAMGATSETGISRPFDRRRDGFVMGEG
                     AGVLVLESEHSASARGAEPIGEVLGYGASSDAHHLTAPDPSGSGAARAVGRALDDAGI
                     GPDDVDYVNAHGTSTPLNDRSETDALKLALGDERAHQVPVSSTKSAIGHLLGAAGAVE
                     AIATVLALRGRVIPPTLGWEERDEGLDLDYVPRESRELELNGSDRAVAISEAFGFGGH
                     NAVLVLAA"
     gene            complement(7784..8032)
                     /locus_tag="VF752_16790"
     CDS             complement(7784..8032)
                     /locus_tag="VF752_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8857254.1"
                     /translation="MPATATPDQIEQTIMDQIAKLGPDRDEIKRDSTFESLEIDSLDL
                     VEVAQVVEDEYGVQIKGEEAEKLNTVGDAVDLVVQRAS"
     gene            complement(8064..8801)
                     /gene="fabG"
                     /locus_tag="VF752_16795"
     CDS             complement(8064..8801)
                     /gene="fabG"
                     /locus_tag="VF752_16795"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX8857255.1"
                     /translation="MRQDHEGCALVTGASRGIGAAIARALAAEGWPVAVNYRSDSEGA
                     DRVVTEIEDAGGQAVAVRGDVSEPGAADDIMRAADERFGPVLVLVNNAGTRADGLSPQ
                     IDDDDWDRVLETNLSGAFRLTRSALRQMMRARFGRIVNVASIVGQRANPGQANYAASK
                     AGLIGFTKTVAAEVARRGVTVNAVAPGFVETELTKGVDQNGFVDAIPARRVGTPEEVA
                     ACVRFLASDEAGYVTGTTLTVDGGLTA"
     gene            complement(8798..9799)
                     /locus_tag="VF752_16800"
     CDS             complement(8798..9799)
                     /locus_tag="VF752_16800"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX8857256.1"
                     /translation="MSELALQAMPLLDEVRDRPARGAALGSVAMAVPERVVPNDPIAE
                     RLGVDSRWIVERTGVRQRHVLEPEARLSDLAADAGGLALERAGLDAADIDLLLVATTT
                     QDELTPNAAPLVATKLGAPRAAAIDVGAACTAFVSALAVAGGQVESGRANNALVIGAD
                     VLYRFVDPDDRRTAALFGDGAGAVIVTAADEARIGPVAMGSDGAHADLIRAGRGEKIF
                     MQGHETFVNAVKRMTEVTVEALRLTGLGIDDIDLFVYHQANSRIIRAVGERLELPPER
                     VVDCVDRYANTSAASIPIALAEAEADGRLFDGARVLLAAFGAGFTWGGTVVVWGDSA"
     gene            complement(9792..10646)
                     /locus_tag="VF752_16805"
     CDS             complement(9792..10646)
                     /locus_tag="VF752_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX8857257.1"
                     /translation="MRTAALFPGQGSQTPDMGATVERLRPDLYELALSALGDDLFDRV
                     DESTAFAQPAIYCASLAGWSRFEDGTPELMAGHSLGEFAALAAAGALDEAEGLRLVAL
                     RGHLMQRAADRHGGGGMLALRADAEGAAALAEQCGMTVANDNAPEQVVLSGPADAMER
                     ATEHASREGIRARPLPVAGAFHSPTMEPAAADFKRALDRADFREPRVPVYSSATAAPF
                     DDIGRRLRESLTHPVRWRETLLAMSRAGAQRFIEVGPGKVLKGLVKRTLPDAKAETLD
                     REPEGAHV"
     gene            complement(10827..12353)
                     /gene="gpmI"
                     /locus_tag="VF752_16810"
     CDS             complement(10827..12353)
                     /gene="gpmI"
                     /locus_tag="VF752_16810"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX8857258.1"
                     /translation="MTKPPVPSVCLVVLDGWGLAEPGPGNAVSLAKTPVFDGLWEKHA
                     HTELKTSGRAVGLPDGQMGNSEVGHLNLGAGAVVRQDLTRIADAIGDGSFFENEVLTR
                     ICAAGRDAGRLHLLGLVSKGGVHSGFEHLRALIELAARSDVPDIVLHAFTDGRDTPPN
                     SGADFVAEAEGWVREAGGRIATVSGRYYAMDRDNRWNRTELAYNAIVRGQSEVPPASS
                     GEEAVRAAYDRDETDEFIKPTLVGDEGRIRDGDAVFFFNFRPDRARQLTRALAEPGFS
                     EFDVGDRPSIEFATLTEYQEDWDYPVAFPPERPSVTLASVLAEHGARQLHVAETEKYA
                     HVTYFFNGGEENEYPGEERKLVDSPRDVPTYDHKPEMSARAASQAFVERWRANGYRFG
                     IINFANADMVGHTGVIPAAVAAVEAVDECLGEVVRAVEESGGELVITADHGNADNMLE
                     PDGSPNTAHSLNPVPCIVTANVAGLREGGILADVAPTVLDLLGEEKPPEMTGESLIER
                     "
     gene            complement(12350..13108)
                     /gene="tpiA"
                     /locus_tag="VF752_16815"
     CDS             complement(12350..13108)
                     /gene="tpiA"
                     /locus_tag="VF752_16815"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX8857259.1"
                     /translation="MPDRRPYVAGNWKMHKTVAETEAYVQELRRMLPESPSAEVGVCV
                     PFTDLVKALEGVAGTDIRVLAQNMHQEQWGAYTGEISAPMLTELGVHGVVLGHSERRQ
                     YFCETDRALQEKVPAALAAGLQPILCVGESEEERDADETQRRLRHQVQEALEKVGDDQ
                     LADVVIAYEPIWAIGTGRVATAAQAQDAIAFIRALVGDRSAQAAERVRVLYGGSVKPD
                     NATEILSEPDVDGALVGGASLDPDDFAAIVAAAA"
     gene            complement(13101..14294)
                     /locus_tag="VF752_16820"
     CDS             complement(13101..14294)
                     /locus_tag="VF752_16820"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX8857260.1"
                     /translation="MGSFGKKTVRDLPVSEGQRVLVRVDFNVPLEEGRVADDARIRAA
                     LPTIEHLRGRGAALVLCSHLGRPMGFDPELSLAPVSKRLGELLDTRVYQAPEVVGPEV
                     RRGAGRLAASEILVLENTRFEPGETKNDPELSRALADLADAYVNEAFGAAHRAHASTA
                     GVTDFLRPAVAGLLLENEVVTLNGVVENPKRPLVVVLGGAKVSDKIALIERFQEIADA
                     ILIGGAMCFSFFRAQGKPTGDSLVEEEGVELARQALERAEKGGARLLLPLDLIIGDRF
                     DADAERRELDGTDVPDGWMGLDVGPRTVEAYSREVADAGTVFWNGPMGAFEIEPFAHG
                     TRGLAEAVAAAPGTTVVGGGDSAAALAKFGLADRVDHMSTGGGASLELLEGRQLPGVE
                     VLDDA"
     gene            complement(14304..15305)
                     /gene="gap"
                     /locus_tag="VF752_16825"
     CDS             complement(14304..15305)
                     /gene="gap"
                     /locus_tag="VF752_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX8857261.1"
                     /translation="MPVRAGINGFGRIGRNVFRAALAADADIEWLGVNDITDTKTLAT
                     LIKYDSILGPFPGTVEATDTGIVVDGKELRVFAERDPAALPWGDLGADVVVESTGLFT
                     DRENAAKHIDAGAKKVIITAPAKGPDVTLALGVNEDAYDKDQHHVISNASCTTNCLAP
                     VAKILHDVFGIEHGLMTTVHAYTSDQRLQDMPHKDLRRARAAAVNLVPTSTGAAKAIG
                     LVIPELDGKLNGIAVRAPVPTGSIVDLVARVNKPTSVDEVNAAFKERADKGALECILQ
                     YTEDPIVSTDIEQSPYSSIFDAGLTMVIDGQLVKVGAWYDNEWGYSNRVVDLVQRVL"
     gene            complement(15393..16334)
                     /gene="rapZ"
                     /locus_tag="VF752_16830"
     CDS             complement(15393..16334)
                     /gene="rapZ"
                     /locus_tag="VF752_16830"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX8857262.1"
                     /translation="MVSWIRPRSRRSDSEQEARRSSIEDFVVITGFSGAGKSQAMACF
                     EDAGYFCVDNLPPEMISSLADLFRHEGSKVERAAVVSDVRGGAYFQSLVQVLDELVEL
                     GTNYRLLFLDASEETLVDRYKETRRRHPLAVGGQVASGIHAERELLEPLRERADVSID
                     TTDMSASRLRKVVADKMLTQQTPGKLAVTFLTYGYKHGAPRDADLAFDVRFLPNPHWE
                     PDLREKTGLDQPVIDYVEGSDGIADFYEKLLPLLDYLLPAYVREGKAHLMIGIGCTGG
                     RHRSVVIAEHLARVYGEREDLLVDVVHRDVHKPPYRP"
     gene            complement(16352..>18233)
                     /gene="uvrC"
                     /locus_tag="VF752_16835"
     CDS             complement(16352..>18233)
                     /gene="uvrC"
                     /locus_tag="VF752_16835"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX8857263.1"
                     /translation="AIDAEPAHLERTERLTAQRRGLPDQPGVYLFRDARGRVIYVGKA
                     KSLKKRVASHFSSPSTRGARDLLEEIDRIEFIVTQTEAEALLAEQTFIRQYRPRYNVR
                     LRDDKSYPYIAISLDEDFPRVYFTREKHRRNRAYFGPFSNAKRVRETLDLLGKVFQYR
                     TCDGAEPGRASGSPCLDYFIKRCQAPCVGYVSKEEYRANIESIVDFLSGRYRQIERDL
                     ERDMRAAADAQEFEQAAAYRNRLKAVRSLLERQRISNEAVGTLDAVAVAVEGTDANAQ
                     VFQVRDGVLADRQSFYLENEAGRDEGEVAEEFVLQYYATSPAIPPQVLVPRSVSQSDV
                     LAEALAERRGAPVEIRHPERGDKRRIFELATRNAQLALDQDRLRSEHRRSQRIEALEG
                     LQQALGLETIPMRIECFDISNLGETHTVASMVVFEGGAPKKADYRRFGVREAVQDDFA
                     AMEEVLSRRMAQFVSQRERSPHEKSFDSSFAAVPTVIVIDGGKGQLSAGLRALQEFRD
                     LGVTVISLAKRIEEVFLPGQREPLQLPHDAPELQLLQRVRDEAHRFAIEFHRGRRDKA
                     MTRSILDELPGVGPARKRLLLKHFGTPERFLQASREELEAVPGLPGKTAREIYEYVHK
                     VR"
BASE COUNT         2635 a         6502 c         6189 g         2907 t
ORIGIN      
        1 cagtgcggct tgtggaggtc catgcgccag cgcgcgtcgg ggtcctggac gagcgcggtg
       61 ccgtgccggc ccaggcgggt tggcgtggcg cagtcgaacg tgtcgatccc cgcttcgacc
      121 gcgtgcacga tgtcgtcgac gtcgccgata ccaagcagat gtcggggcag gtgctcgggc
      181 gttggccgga gcgcccactg cacgacctcg tgcatctcgc ccttctcctg accgagcgag
      241 ccgccgaccg cgattccatc aacgcgggcg ctcgcgaccc gctccgccga ctgctgccgc
      301 aggtcctcgt agacgccgcc ctggatgatc ccgtagaaga gttgccattc gggcgcgtac
      361 tgctcgtgcc agtcgatgca gcggtcgagc cagcggtgcg tgcgctcggt cgaacgggcg
      421 gtgtagtcgc gatccacgtg aaagggcgtg cactcgtcga acgcgagcgc gacgtccgag
      481 ccgagcgcgg cctgggcttc catgctcgtc tccgggccca tgaacttctc gctgccgtcg
      541 agataggact ggaagcggac gccctcctcc tcgatgctca ggatccggct ctggcctggg
      601 cggcgccgct tgatctcctc ggcgatcgag ccatgcccca tcgagaacac ctgaaagcca
      661 cccgagtcgg tgatgatcgg gcgcttccag ttcatgaact cgtgaagccc gcccagcttc
      721 gcgatgtgct cctgcccggg ctggatgaag aggtggaagg tgttgccgag caccatctcg
      781 tagccaagcg cggcgacctc cgcggagctg agccccttca cggtggcagt cgacgcgagc
      841 ggaacgaacg ccggcgttcg gagccgaccg tgctccagat ggagaacgcc cgctcgggct
      901 cggcccgcgc gggcgttgat ctcgaaagtc ggttgcggca ctcgccgcag actacgaact
      961 acctgtcgct tgccgcgccc tcgagcgtct ccggcttgcc ggggtcgttc gcgatgctga
     1021 tgcgccgggg cttgcgctcc tcgggcttcg ggatgcggac ctcgagcacg ccacggtcga
     1081 acttcgcctg gatcttgtcc gggtcgacgc cgtcgggcag cgtcatcgag cggctgaact
     1141 tgccgaagct ccgctcgacg cggtaccagc ccttctcgct ctgctcgtgc tcggcatgcc
     1201 gctcacccga gaggcgaaga gtgccgtcgt gcacctcgat cgcgacgtcg tcctcgctca
     1261 tgcccggtag atccgccttg aggatgaatg aatcctccgc ctcgacgagg tccatcggcg
     1321 gtgaccagcg ctgcacacgg cctcgctcaa acggctcgag cagtgtgttg aagaggcgat
     1381 cgaaatcctg agtgctgaac ggctcaggcc tgaccagaag tgccatttga caacctcctg
     1441 attgggcatt tcttactcct tgacaggata gaaacctgag tcggctgctg tcaactttgg
     1501 acgagacgtc tgagggccga tgtggtcaca ggagcggtac gccctcgggc gcggcatcca
     1561 cccggtcgct ggttcgttac acccgccgtg gtgaaccgcg cgtttcggaa ggccacgacg
     1621 agcccgtgcc gaacgagact gatattgggc acctatacgg tgctcaactt aaaggtcgtt
     1681 ccccagacct tttattcgcg cagctcgcgg tgggccagca cgccgtgatc gacgggatga
     1741 gctctcacgt cgcctcgcgc ttcgccgcgc gccgggccgg cggcgcctca cgcacgaccg
     1801 ccggcagaag gggtagcgcg gttcgcagcg cgaatcgcga agcttcgtag aggcctgcgc
     1861 gcgccggccc gaaaggcagt cccagctcat cgcgtaggcg ctccggcagc atcgccaccg
     1921 ttacgagagc gaatgcttcc gcgaacggtc gggccgccag cgggaccggg ggccgcaggg
     1981 tcgcagcgac gacgtctcgg agcgtgctcg tcaccgctag agagtcgcct tcgagcattt
     2041 cggcgatgta cgcgcggaac gcccgcaggt gcggcggctg gcggtcgagc ggcaccccca
     2101 gcagctctgc gagcgcgcgc tgctcgtcgt agtaccggcg ccgatcgcgc tccgacaggc
     2161 cgccaacgtg tcggtcgtag acgagcaggg cggtgtcgac cagcgtcgcg tgcacccaca
     2221 tcagcagctc ggggtcccgc gcgctgtagc gcgagccggc gggaaagcgg gcgcaaccct
     2281 cgtgcgtcgc gccgttcacg cggccatgca cgtgccagag gtggcgcgcg acggcccggg
     2341 cacgttcctc ggacccgaac acgagcgcgg tcgccagatc cagcgtgcga ttgagccgcc
     2401 cccacgggtc atctcggtac tgcgagtgcg cggcgacgcc cgcggcgatc aatggatgag
     2461 cgacctgcaa tatcagggct cgcccgccgc ccagcacgag cacgttctcg cggttgaccc
     2521 gccaggtaat gctgccggga ccgaagagcc cgtcggcggc catgacgcga tcgtaaccgc
     2581 gcgctcgccc cgcgcgcggc agggcgagcg cgccagttga actcagccga ttatgtggaa
     2641 cacgagcttg aacttggcgc cgcctgccat cgacttcagc tccatgaagc cgcgcgcgtt
     2701 gcggtaccgg cctgtgccgc cggtgatcgc gagcgtgctg ttcttcgcgt cgtagaaggg
     2761 gccctcgacg gtgatctggc ccttcggcag gaaggtcgtc tggttgcact cgaacgccgt
     2821 gccggcgacc gtccggacgc agtagccctg gtcggtgccg accttcgcgg tgtccttcgc
     2881 gttgaacacg tcgttcgcaa aagtgagcag gtcgcccggg ctgtcacccg gcgcgcccgt
     2941 atcggtcgtg gcgacccccg tttggtgctc gatgacggtg acgaccttgc ctccgcgcga
     3001 gtcgccccgc gaatgagact tgcccttgcc ccaagcggcg acggcgagga cgacaagcac
     3061 gattgccgcg agtgcaatcg cgacgctgag tttgcgcttc atggcgctcc tcctcggtca
     3121 ggtgagcacc gtcctaaccg agtggtgaac gctggtcaag cgtttaaacg ttcgcgggct
     3181 ccttctcgtt ttcgtgctct tcagcttccg ctgaggcccc gcggccgtcg agccgcgagc
     3241 ccatcgcgtg atagccaccg tccacgtgga ggatctcgcc cgttatcccg cgcgaccagt
     3301 cggacagcag gaagcaagtg gcggcggcga caggcgtgcg gtcgtcgacg tcccagccga
     3361 gtggcgcctg cgcctgccac gcttcggcga gctcggggaa gccgggaatc ccgcgcgccg
     3421 cggtggtctc gagcggccct gcggacacca ggttcacacg cacgccgtgc ggaccgaggt
     3481 cgcgcgcgag atagcgcgcc acggactcga gtgccgcctt ggagacgccc atccagtcgt
     3541 agatcggcca cgccctggag gcgtcgaagt ccagtcccac gaggctcccg ccgccgctct
     3601 cctggaacag cgggtcgagg ctctcgccga gcgccttcag cgagaaggcg cttgtgagga
     3661 acgcggtcac cgcgctctcc gacggcgtcc gcatgaagtt gccgccgaga gcgtcttcgg
     3721 gagcgaacgc gatcgcgtgc agcgcaccgt cgactccgcc ccagcgctcc tgaagttcct
     3781 gccgcaaccg ttcgaagtgc tcaggctcgt taacgtcgag ctcgagcacc tcgggctcgc
     3841 cctgcagccg ccgggcgacg cgctccgtca ggcgcatggc gcgcccgaag ctcgtgagga
     3901 tcacctcagc gccggcgcgt tgcgcctcct cggcgacggc atagccgatg ctttccttcg
     3961 tcatcactcc cgtgatcagt attcgtttgc cctcgagcgt cacagcggca ccacctccgg
     4021 ggcggccggg ttgacttcgc catcggtctc ctgggtagcc ggcgcgcggc gccagatcgc
     4081 ttcgacctcg gctcgtagga ccgtccggcc gcgctggttc aagaccttcc atgcagtcgt
     4141 gaccgtgccg tgctcctcgt cgatcggctc gcggcgtccc agacggccct cgacatggat
     4201 ggtgtcgccg atgtggaccg gctgcttgaa cacgacgtcg cgcacgcggc gcagcgccac
     4261 gactctgtcc ggatcgagcg gcacgaggcc gactgcgtac gacaagacga gcatcccgtg
     4321 cgcgatccgg ttcccgaagc ggctctcgcc cgcccatgcc gcgtccatgt gctgaggatg
     4381 gaaatcgccc gtcagcgtgg cgaacgaaac gacatcggtc tccgtgatcg tccttcctcg
     4441 cgtgacgaac gactcgccat ccacgagcga gtcgaagttg cgtgtgaaga gattgctcat
     4501 gtgccttcct ccgcccgtcg gattccgtag tgcttctcga gttcgagcgc cgccccccgc
     4561 gccgcctggc ggctgccctc gggcgagggg tggtagccgt cgtcggcgaa gtggccgcgc
     4621 tcggccgcgt ccgggtgctc ggcgaactcc aggcacagca ccccgtagcg gtcggcgacc
     4681 atgcgcgtcg cgtcgttcag ccgctgcatc ccctcggtaa cgcgctggcg ggaccgtggg
     4741 cggagcggca ggaagttggc gaggtcgggc gtcgtgcctg tcacgacgat cgcgtccggc
     4801 acctccgcga gcaggcgctg aagcatctcg tcgagcgcgg ccgcatgcac gtcgatgtca
     4861 ggacgcaccg acagcagcac atcgttggcg ccgcatatga ccgtgacgag gtcggggcgc
     4921 agttcgatgc agggctcgag ctgcgtgccc gcgacctgcg gcgtggtcgc cccagccacg
     4981 cccaggttgt ggtaatccgc gaccgcgagc tcgcgcgcga gctcgtcggg gaatcgcgtc
     5041 tcgtcgagct gcccgggcgc gccgcccgtg aagctgtcgc cgatggccac gtaccgcctg
     5101 accgtccggg cgggccggga gtcgctagag cgggatgttg cccgcacgat ccaaccccct
     5161 gtccttcggc aacgtcgcga ggttcgaggc cagccgctcg cgcgtctccg cgggctcgat
     5221 cacctcgtcg atgaaaccct ccctcgcggc tatctcggcg ttcagatgct cgtcggcgta
     5281 ctccgccgca tacgcgtcgc gcgcggacac ggggtcgtcg gccgcctcga tctcgcgccg
     5341 ccggatgatg ccgacagcct gcgtcgggcc catcacgccg atctcagcgt ccggccaggc
     5401 gtaagcgaag tccgcgccga ggtccttcga gttcatagtg atgtatgcgc cgccgtaggc
     5461 cttgcgaagc acgacggtga gcttcggaac cctggcctcc gcgaacgcgt ggagcagctt
     5521 tgcgccgtga cggatcacgc cagcgcgctc ctgcttcgtc ccaggtagga agcccggtgc
     5581 gtcgacgagc acaaccagcg gcagcccgta gttgttgcac gcgcgcacga agcgggcggc
     5641 cttctgggcc gagtccgcgt cgagcgttcc gccgaggtgc ttcggctggt tcgcgatcac
     5701 cccgaccggc cgcccctcga tacgggcgaa ggccgtgacc acgttccgtg cccatttctg
     5761 cgctgcctcg agcaactcgc cgccgtccgc gatcgcgcgg atcacgtcac gcacgtcgta
     5821 cacacttcgc tcctcagcgg gcacgaagcc ggccggatca tcgcccggag catctatagg
     5881 cgcggaacgc gggggcgact cccaggcgtt ctggggcagg aatgagagca gctcccgcgc
     5941 caggaagatc gagtccgcgt cgcccggcac gacgaactgc gccacgccat ttcgctcgtg
     6001 cacgcgcgcg ccgccgagct cggcgccgga aacttcctcg ccgaggacgt cgcgcacgac
     6061 ggcgggcccg gtgagaaaca tgctcgcccg ttccgtcatc accacgaagt ccgtcagcgc
     6121 cggggagtac gagccgccgc ccgccgaggt ccccccgatc acggagatct gcggcacgcg
     6181 ccccgacagg cgcacgttct cgcggaagat ccgcccgtag ccgccgagtg ccgccgttcc
     6241 ctcctgcata cgcgcgccgc ccgattcgat gaatccgacg acgggtacgc gcgcttcgcc
     6301 cgcgaggcgc atgacgcgca cgatcgtgtc cgcgtgtgcc tgaccgagcg accctccggc
     6361 gaagctggcg tcctgcgcgt agcagtacac cgggcggccg tcgacctcgc ccgacgcgcc
     6421 gatcaccccg tcgcccggac gggccttgct ccccatccgc ggggacgaga cccgcgagcg
     6481 gatcactcgc agcgacgatg cgtcgcacag cagctcgagc cgctcgccgg cggacagccg
     6541 ctccggcgca cgctccaacg tcgtttcgcg ctggctcgtc atcacgctgc gagcacgagc
     6601 accgcgttgt gcccgccgaa tccgaaagcc tccgagatcg cgaccgcgcg gtcgctgccg
     6661 ttcagctcca gctcgcggga ctcgcgcggc acgtaatcga ggtcgagtcc ctcgtcgcgc
     6721 tcctcccagc cgagcgtggg agggatcacc cgcccgcgta gcgcgagcac cgtcgcgatc
     6781 gcctcaaccg cgccagccgc gccgagcagg tgcccgatcg ccgacttggt ggacgacacc
     6841 ggcacctggt gcgcacgctc gtcgccgagc gccagcttga gggcatcggt ctccgagcgg
     6901 tcgttcagcg gcgtggacgt gccgtgcgcg ttcacgtaat ccacgtcgtc cggcccgata
     6961 cccgcgtcat caagcgcgcg tccgacggcc cgcgccgccc cgctcccgga tgggtcgggc
     7021 gccgtgagat ggtgagcatc ggagctcgcg ccgtagccga gcacctcgcc gatcggctcc
     7081 gcgcctcgcg cgctcgcgga atgctcgctc tccagcacga gcacgcccgc gccttcgccc
     7141 atcacgaagc catcgcgccg gcggtcgaag ggtcgcgata taccggtctc cgacgtggcc
     7201 cccatcgcgg cgaaggcggt ggtggcgagc ggcgtgatcg ccgcctcggc gccacccgcg
     7261 accatggcgt cggcgtcgcc tgtccgtatc agccgcgagg ccgtcccgat cgcgtcggcg
     7321 ccggccgcgc atgccgagac cgtgctgaac gccgggcctc ccaggccgtg gcgcatcgcc
     7381 aggccggcgc tgccggcgtt cgccatcatc agcggcaccg cgagcggtga caacttctcc
     7441 gccccacgct cgcgcagcac gtcgtgctgg tcctcgagcg agctgagccc gccgatcccg
     7501 gtacccacga tgcagccgac gcggtcgggc tcgaccggta cctcgtcgct ccagcccgcc
     7561 tgctcgaacg cctcggcgca ggcggcgatg gcgaactgca cgaagcggtc ggtgcggcgc
     7621 agctccttct tcgagagcac atcggccggc tcgaagtcgg agcagcggcc ctcgccatcc
     7681 tcgatgccgc actcgccgcc cgcccagcgc tcgagcaggg tggtggcgcc gacgccgagg
     7741 ggcgtgacgg cgccggctcc cgttatgacg actcgagtgc tcatcacgat gcgcgctgga
     7801 caacgaggtc gacggcatcg cccacggtgt tcagcttctc ggcctcctcg cccttgatct
     7861 gaacgccgta ctcgtcctcg acgacctggg cgacctccac caggtcgagt gagtcgatct
     7921 cgagcgactc gaaggtggag tcacgcttga tctcgtcacg gtcgggcccg agcttcgcga
     7981 tctggtccat gatcgtctgt tcgatctgat cgggtgttgc tgttgcaggc atttgctgcc
     8041 tccttctctc gtggtctcgt gtgtcaggcg gtcaggccgc cgtctacggt caaggtcgtc
     8101 ccggtcacgt agccggcctc gtcggaggcg agaaagcgca cacaggcggc aacctcctcg
     8161 ggcgtcccga cgcggcgcgc gggaatcgcg tccacgaaac cgttctgatc gacgcccttc
     8221 gtcaactcgg tctcgacgaa cccaggcgcg accgcgttca cggtcacacc gcggcgcgcg
     8281 acctcggcgg ccaccgtctt cgtgaagccg atcaggcccg ccttcgacgc ggcgtagttc
     8341 gcctgcccgg ggttcgcgcg ctggccgacg atcgaggcga cgttcacgat gcggccgaag
     8401 cgcgcccgca tcatctggcg cagcgcgctg cgcgtgaggc ggaacgcgcc ggagaggttc
     8461 gtctccagca cacggtccca gtcgtcgtcg tcgatctgcg gcgagaggcc atcggcgcgc
     8521 gtgcccgcgt tgttcaccag cacgaggacg ggcccgaagc gctcgtcggc ggcgcgcatg
     8581 atgtcgtccg ccgcgcctgg ctccgaaacg tcgccgcgca cggcaacggc ctggccgccg
     8641 gcgtcctcga tctcggtgac cacccggtcg gcgccctccg agtcgctccg atagttcacc
     8701 gcgacaggcc agccctcggc ggcgagcgcg cgtgcgattg ctgcgccgat accgcgtgag
     8761 gcgcccgtca cgagcgcaca gccttcgtgg tcttgcctca tgccgaatcc ccccatacga
     8821 cgaccgtgcc accccacgtg aagcccgcgc cgaaggcggc gagcaggacg cgtgcgccgt
     8881 cgaagagacg accgtcggcc tccgcctcgg cgagcgcgat cgggatggat gccgccgatg
     8941 tgttcgcgta gcggtccacg cagtcgacaa cccgctcggg cggtagctcg agccgctcgc
     9001 ccaccgcgcg gatgatgcgg ctgttcgcct ggtggtagac gaacagatcg atgtcgtcga
     9061 tgcccaggcc ggtcaggcgc agggcctcga cagtcacctc cgtcatccgc ttcacggcgt
     9121 tgacgaaggt ctcgtggccc tgcatgaaga tcttctcgcc acggccggcc cggatcaggt
     9181 cggcgtgggc gccgtcggag cccatcgcga ccgggcctat tcgggcctcg tccgcggccg
     9241 tgacgatcac cgccccggca ccgtcgccga acagcgcggc cgtgcgccgg tcgtctgggt
     9301 cgacgaaccg gtacagcaca tcagccccga tcacgagcgc gttgttggcg cggccgcttt
     9361 cgacctggcc gccggcgacc gcgagcgcag acacgaaggc ggtgcaagcc gcgcccacgt
     9421 cgatcgccgc ggcgcgcgga gcacccagct tcgtggcgac cagcggtgcc gcgttgggag
     9481 tcaactcgtc ctgcgtcgtg gtagcaacga ggagcaggtc gatgtcggcg gcgtcgagac
     9541 ccgcgcgctc cagcgccaga ccccccgcgt cggcggcgag gtctgagagc cgcgcctcgg
     9601 gttcgagcac gtggcgctgt cgaacgcccg tgcgctccac gatccagcgg gagtccacac
     9661 cgaggcgctc ggcgattggg tcgttcggca ccactctctc gggcacggcc attgcgacgg
     9721 acccgagggc ggcgccgcgt gcgggacggt cgcgcacctc gtcgagaagc ggcattgctt
     9781 ggagtgcgag ctcagacatg cgcgccctcc ggctcgcggt cgagggtctc ggccttggcg
     9841 tcagggagcg tccgcttcac cagccccttc aagaccttgc cggggccgac ttcgatgaag
     9901 cgctgggctc ccgccctgct catcgcgagc agcgtctcgc gccagcggac ggggtgcgtc
     9961 aggctctccc tcagccgtcg gcctatgtcg tcgaaaggcg cggcggtagc ggacgagtag
    10021 accggtaccc gcggctctct gaagtcggcg cggtcgagcg cccgcttgaa atccgcggcc
    10081 gccggctcca tcgtcggcga gtggaatgcg ccggccaccg ggagcggccg ggcgcggatg
    10141 ccttcacggc tcgcgtgctc ggtcgcgcgt tccatcgcgt cggccgggcc cgacagcacc
    10201 acctgctcgg gcgcgttgtc gttcgcgacg gtcataccgc actgctctgc aagcgcggca
    10261 gcgccttcgg cgtccgcgcg gagcgcgagc atgccgccgc cgccgtggcg atcggcagcg
    10321 cgctgcatta gatggccgcg cagcgcgacc aggcgaaggc cctcggcctc gtcgagcgcg
    10381 ccggcggcgg cgagcgcggc gaattcgccc agcgaatgcc ccgccatcag ctcaggagtg
    10441 ccgtcttcga agcgagacca gcccgcgagg ctcgcgcagt agatggcggg ttgcgcgaac
    10501 gcggtgctct cgtccacgcg atcgaacagg tcgtcgccga gcgcgctgag cgcgagctcg
    10561 tacaggtcgg ggcgcagtcg ttcgacggtt gcgcccatat ccggggtctg gctcccctgt
    10621 ccagggaaca gcgccgcggt gcgcatgtgt tctccctccg gccgtcagcc aatgtgatcg
    10681 cttgcgaagc aatcccttgg ttaggccctt tccggggcgc gcccgcgcat gctcgccacg
    10741 tcccaattcc ccccggtcgg ggggtttcgc gtgcggattg ccacatggcg cctttggagc
    10801 cgcggcggcg cttgaagtag cgagatctac cgctcgatca gcgactcgcc cgtcatctcg
    10861 ggcggcttct cctcgccgag caggtcgagc acggtcggcg ccacatccgc gaggatgccg
    10921 ccctcgcgca ggccggcgac gttggccgtc acgatgcacg gcaccgggtt cagcgagtgc
    10981 gccgtgttcg gtgagccgtc cggctcgagc atgttgtcgg cgttgccgtg gtcggccgtg
    11041 atgaccagct cgccgcccga ctcctcgacc gcgcgcacca cctcaccgag gcattcgtcg
    11101 acggcctcga ccgcggcgac cgcggccggg atcactcctg tgtggccgac catgtcggcg
    11161 ttggcgaagt tgatgatgcc gaagcggtag ccgttcgccc gccagcgctc gacgaacgcc
    11221 tgggaggcgg cccgggcact catctcgggc ttgtggtcgt acgtcggcac gtcgcgcggc
    11281 gagtccacga gcttgcgctc ctcgccgggg tactcgttct cctcgccgcc gttgaagaag
    11341 taggtcacgt gcgcgtactt ctcggtctcg gcgacgtgca gctgcctcgc gccgtgctcg
    11401 gcgagcacgg acgcgagcgt caccgacggc cgctcgggcg ggaaggcgac cgggtaatcc
    11461 cagtcctcct ggtactccgt gagcgtcgcg aactcgatcg atggtcggtc accgacgtcg
    11521 aactccgaga aacccggctc cgccagcgcg cgcgtgagct gacgtgcgcg gtccggccgg
    11581 aagttgaaga agaagaccgc gtcgccgtcg cggattcggc cctcgtcgcc cacgagcgtc
    11641 ggcttgatga actcgtcggt ctcgtcgcga tcgtaggccg cgcgcaccgc ctcctcgccg
    11701 gaagatgcgg gcggcacctc ggactgcccg cgcacaatcg cgttgtacgc gagttcggtg
    11761 cggttccagc ggttgtcgcg gtccatcgcg tagtagcggc cgctgacggt cgcgatacga
    11821 ccgcccgcct cgcgcaccca cccctccgcc tccgcgacga aatccgcgcc cgagttggga
    11881 ggcgtgtcgc gcccgtcggt gaacgcgtgc agcacgatgt ccggcacgtc cgagcgcgcg
    11941 gccagctcga tgagcgcacg caagtgctcg aagccggagt gcaccccgcc cttcgagacg
    12001 aggccgagga ggtgcagccg cccagcgtcc cggccggcgg cgcagatgcg cgtgagcacc
    12061 tcgttctcga agaacgagcc gtccccgatc gcgtccgcga tgcgcgtgag gtcctggcgc
    12121 accaccgcgc cggcgccgag gttgagatgc ccgacctcgg agttgcccat ctgcccgtcg
    12181 ggcaggccga ccgcccgacc ggaggtcttg agctccgtgt gcgcatgctt ctcccacagc
    12241 ccgtcgaaca cgggcgtctt cgccagcgag actgcgttgc ctggcccggg ctccgccagc
    12301 ccccagccgt cgagcaccac gaggcacacc gacggcaccg gcggcttcgt catgcggcag
    12361 cggcgacgat cgccgcgaag tcgtccgggt cgaggctcgc gccgccgaca agcgcaccgt
    12421 ccacgtcggg ctcggacagg atctcggtgg cgttgtcggg cttgacgctg ccgccgtaca
    12481 gcacgcgcac gcgctcggcc gcctgggcgg agcggtcgcc caccagggcg cggatgaatg
    12541 cgattgcgtc ctgcgcctgt gccgccgtcg ccacgcgccc ggtgccgatc gcccagatcg
    12601 gctcgtaggc gatcaccacg tcggcgagct gatcgtcgcc caccttctcg agcgcctcct
    12661 gcacctggtg gcgcaggcgg cgctgcgtct cgtcggcgtc gcgctcctcc tcggactcgc
    12721 ccacgcacag gatcggctgc agccccgcgg cgagggcggc gggcaccttc tcctgaagcg
    12781 cgcggtcggt ctcgcagaag tactggcgcc gctccgagtg cccgagcacg acgccgtgca
    12841 cgcccagctc cgtgagcatc ggtgcggaga tctcgccggt gtaggcaccc cactgctcct
    12901 ggtgcatgtt ctgcgcgagg acgcgtatgt cggtgccggc gacgccctcg agcgccttca
    12961 ccaggtccgt gaacggaacg cagacgccca cctcggccga gggcgactcg ggcaacatgc
    13021 ggcgcagctc ctgaacgtaa gcctccgtct cggcgaccgt cttgtgcatc ttccagttcc
    13081 ccgcgacata cggccgccgg tcaggcatcg tcgagcacct ccaccccagg gagctgtcgg
    13141 ccctcgagca gctcgagcga cgccccgcct ccggtcgaca tgtgatccac ccggtcggcg
    13201 agcccgaact tggcgagagc cgcggctgag tcgccgcccc cgaccacggt cgtacccggt
    13261 gcggctgcga ctgcctccgc aagacccctc gtgccgtgtg cgaacggctc gatctcgaac
    13321 gcccccatcg gcccgttcca gaacaccgtg ccggcgtccg cgacctcgcg cgaatacgcc
    13381 tccacggtgc gcggcccgac gtccaggccc atccagccgt ccggcacgtc ggtgccgtcc
    13441 agctcgcggc gctcggcgtc cgcatcgaac ctgtccccaa tgatcagatc gagcggcagc
    13501 agcaggcgcg cgccgccctt ctccgcgcgc tcgagcgctt gacgcgcgag ctcgacgccc
    13561 tcctcctcga cgagcgagtc accagtcggc ttgccctgcg cgcggaagaa gctgaagcac
    13621 atcgcgccgc cgatcaggat cgcgtcggcg atctcctgga agcgctcgat gagcgcgatc
    13681 ttgtcgctga ccttcgcccc accgagcacc acgacgagcg gccgcttcgg gttctcgacg
    13741 acgccgttga gagtgaccac ttcgttctcg agcagcagcc ccgccacggc cggccgcagg
    13801 aagtcggtga cgcccgcggt ggacgcgtgg gcacggtgcg ccgcgccgaa cgcctcgttc
    13861 acgtaggcgt cggcgagatc ggcgagggcg cgcgagagct ccgggtcgtt cttcgtctca
    13921 cccggctcga agcgcgtgtt ctcgagcacg aggatctcgc tggccgcgag ccgtccggcg
    13981 ccgcgcctga cctcgggccc gacgacctcc ggcgcctgat agacgcgtgt gtcgagcagc
    14041 tcgccgagcc gcttcgagac cggcgcgagc gacaactcgg ggtcgaagcc catgggccgg
    14101 ccgaggtgcg agcacagcac gagcgcggcg ccccggccac gtaggtgctc gatcgtgggc
    14161 aacgccgcgc ggatgcgcgc atcgtcggcg acacggcctt cctcgagcgg gacgttgaag
    14221 tcgacccgaa ccagcacgcg ctgtccctcc gagaccggga ggtcccggac ggtcttcttc
    14281 ccgaatgagc ccacctgcgg tgtctacagc acccgctgga ccaaatccac cacccggttc
    14341 gagtagcccc actcgttgtc gtaccaggca ccgaccttca ccagctggcc gtcgatcacc
    14401 atcgtcaggc ccgcgtcgaa gatcgacgag tagggagatt gctcgatgtc ggtggagacg
    14461 atcgggtctt cggtgtactg aaggatgcac tcgagggcgc ccttgtcggc acgctccttg
    14521 aacgccgcgt tcacctcgtc cacgctcgtc ggcttgttca cgcgcgcgac caggtcgacg
    14581 atcgacccgg tcggcacggg cgcgcgcacg gcgatgccgt tgagcttgcc gtcgagctcg
    14641 gggatcacaa gtccgatcgc cttggccgcg cccgtggagg tcggcacgag gttcaccgcg
    14701 gccgctcgtg cgcggcgcag gtccttgtgc ggcatgtcct gcaggcgctg atccgacgtg
    14761 tacgcgtgca ccgtggtcat caggccgtgc tcgatgccga acacgtcgtg gaggatcttc
    14821 gccaccggcg cgaggcagtt cgtggtgcag gacgcattcg agatgacgtg gtgctggtcc
    14881 ttgtcgtacg cgtcttcgtt gacgcccagc gcgagcgtga catccgggcc cttggcgggc
    14941 gccgtgatga tcaccttctt cgcgccggcg tcgatgtgct tcgcggcgtt ctcgcggtcg
    15001 gtgaagaggc ctgtggactc gaccaccacg tcggctccga ggtcgcccca gggcagcgca
    15061 gccgggtcgc gctcggcgaa cacgcgcagc tccttgccgt cgacgacgat cccggtgtcg
    15121 gtcgcctcga cggtccccgg gaacgggcca aggatcgagt cgtacttgat gagagtggcg
    15181 agcgtcttcg tgtcggtgat gtcgttgaca ccgagccact cgatgtcggc gtcggcggct
    15241 agcgcggcgc ggaagacgtt cctaccgata cggccgaagc cgttgatacc ggcccttacg
    15301 ggcatagacc ttcctttcga gagggggaac cccagcgagg ggcggaatct atcagcgcca
    15361 ggattcccct ccgctgccgc gcgaaacccg gcctacgggc ggtacggagg cttgtggacg
    15421 tcgcggtgca cgacgtccac gagcaggtcc tcgcgctcgc cgtagacgcg cgcgaggtgc
    15481 tccgcgatca ccaccgagcg gtggcggccg ccggtacagc cgatgccgat catcaggtgt
    15541 gccttgccct cgcgcacgta cgccgggagc aggtagtcga gcagcggcag cagcttctcg
    15601 tagaagtccg cgatgccgtc ggagccctcc acgtagtcga tcaccggctg gtcgaggccc
    15661 gtcttctcgc gcagatcagg ctcccagtgc ggattcggca ggaagcgcac gtcgaaggcg
    15721 aggtcggcgt cgcgcggggc gccatgcttg tacccgtagg tgaggaaggt gacggccagc
    15781 ttgcccggcg tctgctgggt gagcatcttg tccgcgacga ccttgcggag gcgcgaggcg
    15841 gacatgtccg tggtgtcgat cgagacgtcg gcgcgctcgc gcagcggctc gagcagctcg
    15901 cgctcggcgt ggatgccgct cgccacctgg ccgccgacgg ccagcgggtg acggcggcgg
    15961 gtctccttgt agcggtccac gagcgtctcc tcactcgcgt ccaggaacag cagccggtag
    16021 ttcgtgccga gctcgaccag ctcgtcgagc acctgtacga gcgactggaa gtacgcgcca
    16081 ccgcggacgt cggacacgac cgcggcgcgc tcgaccttgc tgccctcgtg ccggaacagg
    16141 tccgcgagcg agctgatcat ctctggcggg aggttgtcga cgcagaagta gccggcgtcc
    16201 tcgaaacagg ccatggcttg ggacttgccc gcgcccgaga agccggtgat cacgacgaag
    16261 tcctcgatcg agctgcgccg ggcctcctgc tcggaatccg agcggcgact gcgagggcgg
    16321 atccaggaca ccatgttcac cccactacgg gttatcggac tttgtgaacg tactcgtaaa
    16381 tctcacgggc ggtcttacct ggtagaccgg gcacggcctc gagctcttcc cggctcgcct
    16441 gaagaaaacg ctcgggcgtt ccgaagtgct tgagcaatag tcgcttgcgc gccggaccga
    16501 ctcccggaag ctcgtcgagg atcgagcgtg tcatcgcctt gtcgcggcgc ccgcggtgga
    16561 actcgatcgc gaagcggtgc gcctcgtcgc ggacgcgctg gagcagctgc agctcgggcg
    16621 cgtcgtgcgg cagctgtagc ggctcgcgct gaccgggcag gaagacctcc tcgatccgct
    16681 tcgcgaggct gatcacagtc acgccgaggt cacggaactc ttgcagcgcg cgcaggccgg
    16741 ccgacagctg gcccttgccg ccgtcgatca cgatcacggt cggcaccgct gcgaagctcg
    16801 agtcgaacga cttctcgtgc ggcgagcgct cgcgctgcga cacgaactgg gccatgcgcc
    16861 ggctgagcac ctcttccatc gcggcgaagt cgtcctgcac cgcctcgcgg accccgaagc
    16921 gccggtagtc cgccttcttc ggcgcgccgc cctcgaacac gaccatcgag gccaccgtgt
    16981 gcgtctcgcc aaggttcgag atgtcgaagc actcgattcg catcgggatc gtctcgaggc
    17041 cgagcgcctg ctggaggccc tcgagcgcct cgatgcgctg agagcgccgg tgctcggagc
    17101 gcaagcggtc ctggtcgagc gccagctggg cgttgcgggt cgccagctcg aagatgcgcc
    17161 gcttgtcgcc gcgctcgggg tggcggatct cgaccggggc gccgcgccgc tcggcgagcg
    17221 cctccgcgag cacgtcgctc tgcgacacgc tccgcggcac cagcacctgc ggcgggatcg
    17281 ccggcgaggt ggcgtagtac tggagcacga actcctccgc gacctcgccc tcgtcgcggc
    17341 cggcctcgtt ctcgaggtag aagctctgcc ggtccgcgag cactccgtcc ctgacctgga
    17401 agacctgcgc gttggcatcg gtgccctcga ccgcgaccgc cacggcgtcg agcgtgccga
    17461 ccgcctcgtt cgagatgcgc tgccgctcga gcagcgagcg caccgccttc aagcggttcc
    17521 ggtacgccgc cgcctgctcg aactcctgcg catccgccgc ggcgcgcatg tcgcgctcga
    17581 gatcgcgctc gatctggcgg tagcggccgc tcaggaagtc cacgatcgac tcgatgttcg
    17641 cccggtactc ctccttcgac acgtagccca cgcacggtgc ctggcagcgc ttgatgaagt
    17701 agtccaggca ggggctgccg ctcgcgcggc ccggctcggc gccgtcgcag gtgcggtact
    17761 ggaacacctt cccgagcagg tcgagcgtct cgcgcacgcg cttggcgttc gagaacggcc
    17821 cgaagtaggc gcggttgcga cggtgcttct cgcgcgtgaa gtagacgcgc gggaagtcct
    17881 cgtccagaga gatcgcgatg tacgggtacg acttgtcgtc gcggaggcgg acgttgtacc
    17941 ggggccggta ctggcggatg aaggtctgtt cggcgagcag cgcctcggcc tcggtctgcg
    18001 tgacgatgaa ctcgatccgg tcgatctcct ccagcaggtc gcgcgccccg cgtgtggacg
    18061 ggctcgagaa gtgcgacgca acccgcttct tgagcgactt cgccttgccg acgtagatga
    18121 cgcgcccgcg cgcgtcgcgg aacaggtaga cgccgggctg gtcgggcagt ccgcgccgtt
    18181 gtgccgtgag gcgttccgtg cgctcgaggt gggccggttc ggcgtcgatg gcc
//
LOCUS       DASVXS010000300         8762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_616226, whole genome shotgun sequence.
ACCESSION   DASVXS010000300 DASVXS010000000
VERSION     DASVXS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616226"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF752_16840"
     CDS             complement(<1..563)
                     /locus_tag="VF752_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8857264.1"
                     /translation="MARLSPFAGFGARDMAIDLGTANTLVYVRGRGIVVSEPSVVAID
                     SQTGEVHAVGSEAKRMIGRTPASISAIRPLRHGVIADFEVTEKMLRYFIRKIEQRRMS
                     RPRLVMCAPSGVTDVEKRAVIEASLSAGARQVHLIEEPIAAAIGAGLPIEEPVGNMVV
                     DVGGGTSEVAVISLGGIVVSEPSVVAI"
     gene            complement(582..1409)
                     /locus_tag="VF752_16845"
     CDS             complement(582..1409)
                     /locus_tag="VF752_16845"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX8857265.1"
                     /translation="MSAEDLSRRLPEVDASEPGGEGSGSLEGVPFPPPPRDDEIARLR
                     DIEFPIGLRGYDRDAVDSYVRRVNRLIAELEVSRSPQSAVRHALEQVGEQTRDILQQA
                     HEAAERITAQSRAKADERMRQAEREAGDLRLAAEQTVHSLDTDVEAIWQERQRLIDDT
                     RRLADEMLRVADDASDRFPAVPVTDEQEAPEPQPEPEPEPEPEPDEVEPREEPPETEF
                     HAPADTELTGPQMPAPPQMPAGTETRGDDEEASDEEAFDDETLPIERDDPGEADRAP"
     gene            complement(1480..2433)
                     /locus_tag="VF752_16850"
     CDS             complement(1480..2433)
                     /locus_tag="VF752_16850"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8857266.1"
                     /translation="MTDSTIRTTPFGFESTAAEVVAGIDLDGKRAIVTGGSSGIGVET
                     ARTLASAGAEVTITGRNLDAGANVVADIRATTANPAVDLQALDLADQASVAAFADSWS
                     GPLHILVNNAGIMAVPELQRTKEGWELQFATNHLGHFALAVRLHDALAEAGNARIVSL
                     SSRGHHYSPVVFEDINFESRPYDPWLAYGQSKTANVLFAVEAARRWAGDGITANAVHP
                     GGIHTNLMRYMDPETVENTVNNFRRGLKTPEQGAATSVLVATSLQLEGIGGRYFEDCN
                     EAVVVEPGGPDDRRAGVVTYALDPDNASRLWRVSEDLIAAA"
     gene            2546..2619
                     /locus_tag="VF752_16855"
     tRNA            2546..2619
                     /locus_tag="VF752_16855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2580..2582,aa:Arg,seq:acg)
     gene            complement(2632..3375)
                     /locus_tag="VF752_16860"
     CDS             complement(2632..3375)
                     /locus_tag="VF752_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8857267.1"
                     /translation="MAAVASPLVRIEQLRKEFQAIVAVDELTLDVDSGEIVALVGDNG
                     AGKSTVVKCLAGVHSPTSGRILLDGEPAHFSGPLDARGNGIDVVLQDLALADMQPVYM
                     NVFLGRELVTGTLRRLDRRAMAAETGRLLDELGVHIDSPRQQIRSLSGGQRQGVAIAR
                     AMRWAQRLVLMDEPTAALGIAETERVEELIRRMRDQGRAILLVSHSLDQVFRLADRIC
                     VLRRGRQVGIRSTGETTGDEIVSMITGLR"
     gene            complement(3380..4351)
                     /locus_tag="VF752_16865"
     CDS             complement(3380..4351)
                     /locus_tag="VF752_16865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8857268.1"
                     /translation="MAPRTGIRQSLVPRAIQPRSYFVFAAFLLVFAFFAVVLHDDGFT
                     SQTNLLNIVRQTAPITVMAVATVFVLSAGEIDLSLGSVVALSALVTAKVMQTQDAALA
                     VAAGLGIGVGIGVLNGLFVTLLRLPSFLVTLASMGIVAGLARTVTDLQSIAVENATFV
                     NIFGSGDVGAIPGLAFWGLGAVALGQFGFRHTRFGAHVVATGDNAGAARVAGIRVDRV
                     KLAVLALSGAAAALAGILYTGRLEGATYTLGAQDLLVVIAAVVIGGTRLFGGEGSVIG
                     ALLGSLLLGMLTNGLILWGFSTAQQLIAQGVILLVAISLTLREPRRE"
     gene            complement(4357..5487)
                     /locus_tag="VF752_16870"
     CDS             complement(4357..5487)
                     /locus_tag="VF752_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8857269.1"
                     /translation="MTRSASRVAALAAGVLAMAVAVASCSSNEGASEGSLPIKSSRAV
                     GATRGPHGERAIPTSALDLSPAEVAKVRSGHYTAALVWHEESDFTTAVTAGVRDVFDQ
                     LGIRLVAQTSANFDPAKQKSDLETVEAKKPSVLLSLPIDPVITASAYKAIAGQGTKVV
                     LLSIVPQGMRPGRDYVDMITDDLFEMGKRAADALAAAVGDKGKVGYFFRDANSFVANQ
                     RDQAFLKTITANYPNVKVVARQGIADPNRSEELANAALTKNPDLDGVYVTFSEPPGEG
                     VLAALHSTGNTKTKVVSLDLSEPMALDMAKGGPTFALIADRTYELGRAMATSAGYGLI
                     GKRAPPFLVAPALTITKSNLVQGYRASLHRAPPASVTKALGQ"
     gene            5566..7722
                     /locus_tag="VF752_16875"
     CDS             5566..7722
                     /locus_tag="VF752_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX8857270.1"
                     /translation="MARGQARIGVDPGRLAEEQAALRRVATLVAEGAPPAGVFAVVAE
                     QVAEVLGVPVCSVLRYEPDGTASERASFSPRGELFEVGRHWSLDGTNVVAQVLHTGRP
                     ARIDDYTGLEGTIVEQVRLAGINSTVGIPIVVAGRVWGAMVVSSDDRQPLPEETEARL
                     QDFTELVATAIANSEARAEVERLADAQAALRRVATLVAQEAPREEVFTAITEELVRLL
                     GVEGVRAARFEDDGTATVVGCWGEPHDVLQVGYRIPLEGDSATSIVARTGQTARIENY
                     ATARGEVGQVAREGGLRSAVAAPIVVEGRLWGAMVAASIQEQPLPPETESRLGEFTEL
                     MATAIANGEARAEVERLAEEQSALRRVATLVAEGAPPRAVFDAVATEAAQVLDAESVA
                     LSRYEGDDAVTVVAYQGSGAHRVRAGMRLPVDGQGVTRAVQQTGRPARFDDFAHAEGH
                     IAQLARDLGVSSSVGAPIVVDGRLWGVIVASWTQGHVAPADAEPRLAQFAELLDTGIA
                     NADSRAQLMASRARMLAAGDDARRRVVRDLHDGAQQRLAHSIVTLKLAQRSLEENDAR
                     AETLLEEALDHAERGNAELRELAHGILPSVLTRGGLLAAVDALVSRVDLPVRTDVSCG
                     RLTADVEASAYFIVAEALTNVVKHSRAENAEVRAFVDAGALRIEVRDDGVGGANLEGH
                     GLVGIGDRVAALGGSVRIESPPDGGTLLAAALPLSG"
     gene            complement(7719..8384)
                     /locus_tag="VF752_16880"
     CDS             complement(7719..8384)
                     /locus_tag="VF752_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8857271.1"
                     /translation="MVAPTRVVIGEDDVLLREGTARLLAEAGFDVVGQAGDAEDFLRK
                     ALAHKPDVAVVDIQMPPGHGDDGLQAALELRQRVPQVGVLVLSQYYDDRYAVELIGDS
                     AEGVGYLLKERVGDVEAFTDAVARVAAGGSALDPEVVGRMLGRRRRNGPLDELSPREH
                     DVLAQLAEGKSNQGIAEALVVTNAAVEKHVTSIFQKLGLETSATGHRRVLAALTYLRE
                     PRL"
     gene            complement(8430..>8762)
                     /locus_tag="VF752_16885"
     CDS             complement(8430..>8762)
                     /locus_tag="VF752_16885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8857272.1"
                     /translation="DDPAFLSLAARVIEGMDVDVVTTAPDAAHAIKAAKSTRPDAVLV
                     DVGLPDREGIDLAYELAALPWAPRVVLTSTDRDAVSAIDGERAPGLPFIPKEELANST
                     LRRLLLPD"
BASE COUNT         1305 a         3052 c         3033 g         1372 t
ORIGIN      
        1 tcgatcgcga ccaccgacgg ctccgacacg acgatgccgc cgagcgagat caccgcgacc
       61 tcgctcgtgc cgccgcccac gtccacgacc atgttgccca cgggctcctc gatcggcagg
      121 cccgcgccga tcgccgccgc gatcggctcc tcgatcaggt gcacctggcg ggcgccggcc
      181 gagagcgacg cctcgatcac cgcgcgcttc tccacgtcgg tcacacccga cggcgcgcac
      241 atcacgagcc gcggccgcga catgcgccgc tgctcgatct tgcggatgaa gtagcgcagc
      301 atcttctccg tcacctcgaa gtcggcgatc acgccgtggc gaagcggccg gatcgccgag
      361 atcgacgccg gcgtgcggcc gatcatgcgc ttggcctcgc tgcccacggc gtggacctcg
      421 ccggtctgcg agtcgatcgc gaccaccgac ggctccgaca cgacgatgcc gcggccgcgc
      481 acatagacga gcgtgttggc cgtgccgaga tcgatcgcca tatcgcgagc gccgaatccg
      541 gcgaagggag agagcctggc catctggaga ctctatgcgc cctagggcgc gcggtcagct
      601 tcgccggggt cgtcgcgttc gatcgggagc gtctcgtcgt cgaacgcctc ttcgtcggac
      661 gcctcttcat cgtcgccccg ggtctcggtg ccggctggca tctgaggcgg cgccggcatc
      721 tgcgggcccg tcagctcggt gtcggctggg gcgtgaaact ccgtctccgg cggctcctcg
      781 cgaggctcga cctcatccgg ctccggctcc ggttccggct cgggctcggg ctgcggctcg
      841 ggcgcctctt gctcgtcggt caccggcaca gccgggaagc ggtcggaggc gtcgtcggcc
      901 acgcgcagca tctcatcagc gagccggcgc gtgtcatcga tcagccgctg tcgctcctgc
      961 cagatcgcct ccacgtcggt gtcgaggctg tgcacggtct gttcggccgc cagccgcagg
     1021 tcgcctgcct cgcgctcggc ctggcgcatc cgctcgtccg ccttcgcgcg cgactgggca
     1081 gtgatccgct cggccgcctc gtgcgcctgc tgcaagatgt cgcgcgtctg ctcgcctacc
     1141 tgctctagcg cgtgtcgcac cgcggattgc ggcgagcggc tcacctcgag ctcggcgatc
     1201 aggcggttta cccggcgcac gtaggagtcg accgcgtccc ggtcgtaacc gcgcagtccg
     1261 atcggaaact cgatgtccct gagccgcgcg atctcgtcgt cgcggggcgg cggcgggaag
     1321 gggacgccct cgagcgaacc gctgccctcg ccgccgggct cggaggcgtc gacctcaggc
     1381 agccggcggg atagatcctc cgcgctcatc gactcccgag tgttgcaaac ggggcgcgat
     1441 gacaccgtca ggtgccgccc gaaaagggcg agagctcggt caggcggcgg cgatcaaatc
     1501 ttcggacacc cgccagagcc ggctggcgtt gtccgggtcg agcgcgtatg tgacgacgcc
     1561 ggccctgcga tcgtcgggac cgcccggctc taccacgacc gcctcgttgc agtcctcgaa
     1621 gtagcgtccg ccaatcccct ccagctggag tgatgtggcg accaacacgc ttgtggcggc
     1681 gccctgctcg ggtgtcttga gcccgcgtcg gaagttgttg acggtgttct cgacggtttc
     1741 gggatccatg tagcgcatca ggttggtgtg gatcccgccc gggtggaccg cgtttgcggt
     1801 gatgccgtcg ccggcccagc gacgcgcggc ctccaccgcg aacagcacgt tcgcggtctt
     1861 cgactgcccg taggcgagcc acgggtcgta cgggcgcgac tcgaagttga tgtcctcgaa
     1921 cactacgggt gagtagtggt ggccgcgcga gctgagcgac acgatccgcg cgttgcccgc
     1981 ctctgcgagg gcgtcatgca gccgtactgc gagcgcgaag tggccgaggt gattggtcgc
     2041 gaactgaagc tcccacccct ccttcgtgcg ttgtagctcg ggcaccgcca tgatgcccgc
     2101 gttgttgaca aggatgtgga gcgggccgct ccagctgtcc gcgaacgccg ccacggacgc
     2161 ctggtccgcg aggtcgagcg cctgaaggtc gacggcagga tttgcggtcg tggccctgat
     2221 gtcggcgacc acgttggcgc cggcgtccag attgcgaccg gtgatcgtca cctcggcccc
     2281 cgcgcttgcg agggtgcggg cggtctcgac ccctatgccc gacgagccgc cggtcacgat
     2341 ggcgcgcttg ccgtccaggt cgatacccgc caccacctcg gcggcagtcg attcgaagcc
     2401 gaacggcgtg gtgcggatgg tcgagtcggt catacactca ccgtaggcgg ctggaaagcc
     2461 cacccgatcg gcttttcctc tcgggtgatc gcgtgtcgcg atcccgcgcg ttcctagaga
     2521 tgctcgctag gctctgtgcc tgcaggcgcc cgtagctcag ctggatagag cgtcggtcta
     2581 cggaaccgaa ggtcagaggt tcgaatcctc tcgggcgcgt tttcccgctg cttagcgaag
     2641 gcccgtgatc atcgagacga tctcgtcgcc cgtcgtctcg cccgtgctgc gaatgcctac
     2701 ctgccgaccc cgccgcagca cgcaaatgcg gtcagccagg cgaaacacct ggtcgagcga
     2761 gtggctcacg agcaggatcg cccggccctg gtcgcgcatc cgccggatca gctcttcgac
     2821 tcgctccgtc tccgcgatgc cgagcgccgc ggtcggctcg tccatcagca cgagccgctg
     2881 cgcccagcgc atcgcgcgtg ctatcgcaac tccctgacgc tggccgccgg agaggctccg
     2941 gatctgctga cgcgggctgt cgatgtgcac gccgagctcg tcgagcagtc ggcccgtctc
     3001 agctgccatc gcgcgccggt cgagccgccg cagcgtcccc gtcacgagct cacgcccgag
     3061 gaagacgttc atatagaccg gctgcatatc cgcgagcgcg aggtcctgaa ggacgacgtc
     3121 gattccgttt ccgcgcgcgt cgagtgggcc ggagaagtgg gcgggctcgc catcgaggag
     3181 gatccggccg gaggtaggcg agtggacacc ggccaggcac ttcacgaccg tcgacttgcc
     3241 ggcgccgttg tccccgacga gcgcgacgat ctcgccggag tcgacgtcga gcgtcagctc
     3301 gtcgaccgcg acgattgcct ggaactcctt ccggagctgc tcgatgcgca cgagtggaga
     3361 cgcgaccgcg gccatccggc tactcccgcc tcggttcccg gagcgtgagc gagatcgcga
     3421 cgagcaggat cacgccctgc gcgatcagct gctgcgccgt cgagaagccc cagaggatca
     3481 ggccgttggt gagcatcccg agcagcaaac tgccgagtag cgcgccgatc acgctgccct
     3541 cgccgccgaa gagccgtgtg ccgccgatca ccacggctgc gatcaccacg aggaggtcct
     3601 gagcgccgag ggtgtacgtc gccccctcga gccggccggt gtagaggatt ccggccagcg
     3661 cggccgcggc gccgctcagc gcgagcaccg cgagcttcac ccgatcgacg cggatgcccg
     3721 ccacgcgcgc cgcgcccgcg ttgtcgccgg tcgcaaccac gtgcgcgccg aacctggtgt
     3781 ggcggaaccc gaactgacca agggccactg cgccgaggcc ccagaacgcg agccccggga
     3841 tcgcgcccac gtctcccgac ccgaagatgt tgacgaacgt ggcgttctcg accgcgatcg
     3901 actgaaggtc ggtgacggtt cgcgcgaggc cggcgacgat ccccatcgag gccagcgtga
     3961 cgaggaagga cgggagccgc aggagcgtga cgaagaggcc gttcagtact ccgattccga
     4021 ctccgatgcc gaggcccgca gcgactgcaa gcgcggcgtc ctgggtctgc atcaccttgg
     4081 cggtcaccag cgcggagagg gcaaccaccg atccgagcga caggtcgatc tcgcctgcgc
     4141 tcaggacgaa caccgtcgcg accgccatca cggtgatcgg cgccgtttgc ctcacgatgt
     4201 tcaggagatt tgtctggctg gtgaagccgt cgtcgtgaag gacgacggcg aagaacgcga
     4261 agaccaggag aaacgccgcg aacacgaagt acgagcgcgg ctgaatggcg cgcgggacga
     4321 gtgattggcg gatgcctgtt cgcggggcca agcgcgctac tggccgaggg ccttcgtaac
     4381 gctcgccggc ggtgcgcggt ggagcgatgc gcggtagccc tggacgagat tcgacttcgt
     4441 gatcgtcagt gccggggcca ccaggaacgg cggagccctc ttgccgatca gcccgtaccc
     4501 cgccgatgtg gccatcgccc ggcccagttc gtacgtgcga tccgcgatca gcgcaaatgt
     4561 cggcccgccc ttcgccatgt cgagcgccat cggctccgac aggtcgaggc tcacgacctt
     4621 cgtcttcgtg ttgccggtgg aatgcagcgc ggccagcacg ccttcgcccg gcggctccga
     4681 gaaggtcacg tacaccccgt ccaggtcagg gttcttggtg agagccgcgt tcgcgagctc
     4741 ctccgaccgg ttcggatcgg cgatcccctg cctcgccacg accttcacgt tcgggtagtt
     4801 ggcggtaatc gtcttcagaa acgcctgatc acgctgattg gcgacaaacg agttggcgtc
     4861 gcggaagaag taccccacct tccccttgtc gccgaccgca gccgcgagcg cgtccgccgc
     4921 gcgcttcccc atctcgaaca gatcgtcggt gatcatgtcc acgtagtcgc gccccggccg
     4981 catgccctgg ggcacgatcg agagcagcac gaccttcgtt ccctgtccgg cgatcgcttt
     5041 gtaagccgag gcggtgatca ctgggtcgat cggcaggctc aggagcaccg atggcttctt
     5101 cgcctcgacc gtctcgagat cggacttctg cttcgcgggg tcgaagttcg cgctcgtctg
     5161 cgcgacgagc ctgatgccga gctgatcaaa cacgtcgcgg acgcccgcgg tcacggcggt
     5221 ggtgaagtcc gactcctcgt gccacacgag cgcggccgtg tagtgaccgg accgcacctt
     5281 tgcgacttcg gccggactga gatcaagcgc gctcgtgggg atggccctct cgccgtgcgg
     5341 cccgcgcgtc gcgccaaccg ccctcgagga tttgatcggc aagctgccct cggaggcgcc
     5401 ctcgttgctc gagcacgacg caacggcgac ggccatggca agcacgcctg cggcgagagc
     5461 agcgacccgg ctggcgctcc tggtcacgtg cacgattatc cgttaccgtc agcgccatgg
     5521 cacgtcggcg ctgccatttc gcgcccgtcg attccggttg aggccgtggc aagagggcag
     5581 gccaggatcg gagttgatcc cgggcggctt gccgaagagc aggcggcgct gcggcgagta
     5641 gcaaccctgg ttgccgaggg agcaccaccc gccggcgtat tcgcggtcgt cgccgagcag
     5701 gtagccgaag tgctcggagt cccggtatgc agcgtcttgc ggtacgagcc ggacggcacc
     5761 gcctcggagc gtgccagctt ctctcctcgc ggtgagctct tcgaggtcgg aaggcactgg
     5821 tcgctcgacg gcacgaacgt cgtcgcccag gtgctccata ccggccggcc ggcccgcatc
     5881 gatgactaca cggggctcga ggggacgatc gtcgagcagg tccgcctggc ggggatcaac
     5941 tccaccgtcg gcatccccat cgtggtggcg ggccgtgtct ggggagcgat ggtggtctcg
     6001 tcggacgacc ggcagcccct ccccgaagaa accgaggcgc ggctccagga cttcaccgag
     6061 cttgtcgcca cggcgatcgc gaacagcgag gcgcgtgccg aggtcgagcg tctcgccgat
     6121 gcgcaggccg cgctgaggcg ggtggcgacg ctcgtcgcgc aggaggcacc ccgagaggag
     6181 gtcttcaccg ccatcaccga agagctggtg cgcctcctcg gcgtggaggg cgttcgcgcg
     6241 gcgcgattcg aggacgacgg cacagccacg gttgtgggct gctggggcga acctcacgac
     6301 gtcttgcagg tgggctaccg catccctctc gagggcgata gcgccacgtc gatcgtggcc
     6361 cgcacgggac agaccgcccg gatcgagaac tacgcaaccg cgcgcggcga ggtcggccaa
     6421 gtcgcccgcg aaggcggtct tcgctcggcg gttgcggcgc cgatcgtggt cgagggccgg
     6481 ctgtggggcg cgatggtcgc ggcctcgatc caggagcagc cgcttccgcc ggagaccgag
     6541 tcgaggctcg gcgagttcac cgagctgatg gcaactgcga tcgcgaacgg ggaggcgcgc
     6601 gccgaggtgg agcggctcgc ggaggagcag tccgcactgc ggcgggtggc gacgctcgtc
     6661 gccgagggcg cgcccccgcg cgcggtgttc gatgccgtgg cgaccgaggc ggcacaggtg
     6721 ctggacgccg agagcgtcgc gctgtcccgc tacgagggcg acgacgcggt cacagtcgtc
     6781 gcgtaccaag ggtcaggcgc acacagggtc cgcgccggga tgcgcctgcc tgtcgacgga
     6841 cagggcgtga cgcgcgccgt ccagcagacg ggccgtccgg cgcggttcga cgacttcgcc
     6901 cacgccgagg gtcacatcgc acagcttgcc cgggacttgg gcgtcagctc gtcggtcggc
     6961 gcgccgatcg tcgtggatgg ccgcctatgg ggcgtgatcg tcgcaagctg gacccaggga
     7021 catgtcgcgc cggccgacgc ggaaccgcgg ctggcgcagt tcgcggagct gctcgacact
     7081 gggatcgcga acgcggacag ccgcgcgcag ctgatggcct cgcgggcgcg catgctcgct
     7141 gcgggcgatg acgcgcgccg ccgcgtcgtg cgcgacctgc acgacggggc gcagcagcgc
     7201 ctggctcact cgatcgtcac gctcaagctg gcgcagcgct cgctcgagga gaacgacgca
     7261 cgcgcggaga cgctcctcga ggaggccctc gaccacgccg agcgcggaaa cgcggagctc
     7321 cgcgagctcg cgcacgggat cctgccctct gtgctgacgc gcggcggtct gctcgcggcc
     7381 gtggacgcgc tggtgtcgcg agtcgacctg ccggttcgca cggacgtgtc gtgcgggcgg
     7441 ctaaccgccg atgtcgaagc aagcgcgtac ttcatcgtcg ccgaggcgct cacgaacgtg
     7501 gtcaagcact cgcgcgcgga gaacgcggag gtgagggcct tcgtggacgc cggcgcgctc
     7561 cgcatcgagg tgcgcgacga cggagtcggc ggtgcgaacc tcgaggggca cggcctggtg
     7621 gggatcggag acagggtcgc cgcgctgggc ggatcggtgc ggattgaaag cccgcccgac
     7681 ggcgggacgc tgctcgccgc cgctctcccg ctctcgggct agagcctcgg ctcgcggaga
     7741 taggtcaaag ccgcgagcac gcggcggtga ccggtcgcgc tcgtctcgag gccgagcttt
     7801 tggaagatgc tcgtcacgtg cttctcgacc gcggcgttgg tcaccacgag cgcctcggcg
     7861 atgccctggt tcgacttgcc ctccgccagc tgtgcgagca cgtcgtgctc cctgggactc
     7921 agctcgtcga gcgggccgtt gcgacggcgc cggccgagca tccgcccgac cacctcgggg
     7981 tcgagggcgc tgcctccggc agccacccgc gcgaccgcat ccgtgaacgc ctccacgtcg
     8041 ccgacccgtt ccttcaggag gtatccgacc ccctcagcgc tgtcgccgat cagctcgacc
     8101 gcatagcggt cgtcgtagta ctgagacagg acgagaacgc ctacctgcgg tacccgctgc
     8161 cggagctcga gcgcggcttg gaggccgtcg tctccgtggc caggcggcat ctggatatcg
     8221 acgacggcca catccggctt gtgcgcgagt gccttgcgaa ggaagtcctc cgcgtcgcct
     8281 gcctgcccga ccacgtcgaa gcccgcctcc gcgagcagac gcgcggtccc ttcgcgtaga
     8341 aggacatcgt cctcgccaat gacgaccctg gtcggcgcga ccacgaccgc caagttaccc
     8401 ctcgcgcgac cccagttcca gtgcgctccc tagtcaggta aaaggaggcg gcgcagcgtg
     8461 ctgttcgcca gctcttcctt ggggatgaac ggcagtcccg gcgcccgctc gccgtcgatc
     8521 gcgctgactg cgtcccggtc ggtggaagtg agcaccacgc gaggagccca cggcagcgca
     8581 gccagctcgt aggccaggtc gattccctcg cggtccggga gccccacgtc cacgagtacc
     8641 gcgtccggtc gtgtcgattt ggcggccttg atcgcgtgcg ccgcatccgg cgcggtcgtc
     8701 accacatcga catccattcc ctcgataacc cgcgccgcga ggctcaggaa agccggatcg
     8761 tc
//
LOCUS       DASVXS010000301         3274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_847320, whole genome shotgun sequence.
ACCESSION   DASVXS010000301 DASVXS010000000
VERSION     DASVXS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847320"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VF752_16890"
     CDS             <1..444
                     /locus_tag="VF752_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857273.1"
                     /translation="HVAAHHKHAKHHASQPARIAPATGAPAVAPRAHELGPQPISSSR
                     TPAEPSKRAADKRSGPAATGTQPNDGSTTGNTTAPSVPKGTTDEPGAQPITGSEPVES
                     QGFRSPSQPPAAGGSPTTHAGDTAPPASSGATPVDSSNTYSEPAP"
     gene            489..1841
                     /locus_tag="VF752_16895"
     CDS             489..1841
                     /locus_tag="VF752_16895"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8857274.1"
                     /translation="MSWHVAIAGGGFGGLYAARRLEHVLPKESARITLVSDTNFLLYT
                     PLLPGAASGTLEPRHVVVPLRQELHHTSIRLGWVTGADPTVNELRYRTADERDESLRY
                     DQLIVALGSVSRVLPIPGLAEHGLGFKTISEAIALRNRVLVSLELAEAADDPQLRREY
                     LTFAFVGAGYAGVEGIAELQDFVADVIDRYPRSRLEGTRWILVEAQDRIMMEIAPSLA
                     EFAMRDLRGRGIEVRTGTRLDAVEESAVVLSTGERIPCRTVCWTAGVKPPALVRDLGL
                     PLTHEGRIDVDENMRVRGRENVWAIGDSAAVPDPAKSRTQPSPPTAQHALRQGKRVAE
                     NVAATLGHGHIRPFTYKTLGVFVDMGRHKAVAEVLGVRLRGFPAWWAARTYHLAAMPG
                     VGRRLRLLVDWTVALLFQRDSAELGQLGHPPSLRGYLETPSPEPKEGETAVREAGSGS
                     "
     gene            1853..2791
                     /locus_tag="VF752_16900"
     CDS             1853..2791
                     /locus_tag="VF752_16900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8857275.1"
                     /translation="MTLRGGTNRDLEATHALSQRAIFEAAVKRGTLPPDTKPNPDETR
                     AEWKRQRGLIEYLAAQPSGRYCIAEKDGELVGYGRVVRFDRVEQLTELMVEPGHQGEG
                     IGRAILESCWPGDPSPDLGRLVIATGEPNDLGLYMSFGVMPVCGHWQMRQGTAAFVER
                     RSQELDRQETGVHVLKPDRALAEWRRMEPGALGFSRPGLHEHFARDRTCLATFDAEAS
                     RATSLCWVSSEGEIGPAVAESSQALVPVVLAVLDRVAATQEPEFLSVFSTTVSWALLR
                     RLRVLGFEIYWPSWIMSSKPLPGLDRYVPTRPPHVL"
     gene            2805..2978
                     /locus_tag="VF752_16905"
     CDS             2805..2978
                     /locus_tag="VF752_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857276.1"
                     /translation="MREAASDFGRKALAVLVLLLAAWILFKIVLGFAAVLFWVVLAVL
                     AVLAVLWAVNQLL"
     gene            2989..>3274
                     /locus_tag="VF752_16910"
     CDS             2989..>3274
                     /locus_tag="VF752_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857277.1"
                     /translation="MQGTAFVVIAFFFGLSAGTIGKIKGSSFFLWFAIGFVLPILGTL
                     AALFYRFDRHEPKRRCPECGNVLHVHDQVCMRCGRDLDFPGAEEEAQPAAR"
BASE COUNT          467 a         1169 c         1155 g          483 t
ORIGIN      
        1 cacgtggcgg ctcatcacaa gcacgcgaag caccacgctt cgcagcccgc gcggatcgca
       61 cccgcgacgg gcgccccggc cgtcgccccg cgggcacacg agctcggccc gcagccgatc
      121 agctcgtcgc gcacgccggc cgagccctcg aagcgggccg ccgacaagcg cagcgggccc
      181 gccgcgacgg gcacgcagcc caacgacggc agcaccaccg gcaacacaac cgcgcccagc
      241 gtcccgaagg gcacgaccga cgagccgggc gcccagccga tcaccgggtc cgagccggtc
      301 gagagccagg gcttcaggtc gccgtcacag ccgcccgcgg ctggcggctc tccgaccacg
      361 cacgccggtg acaccgcgcc ccccgcctcg tcgggcgcaa cgccggtcga ctcgagcaac
      421 acctactccg agcctgcacc gtaggcgcgc gtttagccgc cttgtgcccc caggtagcct
      481 ggggcctgat gtcttggcac gtcgcgatcg cgggcggtgg cttcggcggc ctctacgcag
      541 cgcgcaggct cgagcacgtg ctgccgaagg agagcgctcg catcaccctc gtgagcgaca
      601 cgaacttctt gctctacacg ccgctgctgc ccggcgcggc atcgggcacg ctcgagccgc
      661 gccacgttgt ggtgccactc cgccaagagc tgcatcacac gagcatccgc ctcggctggg
      721 tcaccggcgc ggacccgacg gtcaacgagc tccgctaccg cacggcggac gagcgggacg
      781 agtcgctcag gtacgaccag ctgatcgtcg cgctcgggtc ggtgtcgcgt gtcctgccaa
      841 tcccggggct ggccgagcac ggccttggct tcaagacgat ctccgaggcg atagcgctcc
      901 gcaaccgcgt gctcgtcagt ctcgagctcg ccgaggccgc tgacgacccg cagctccggc
      961 gcgagtacct gacgttcgcg ttcgtcggcg caggctatgc cggggtggaa gggatcgcgg
     1021 agctgcagga cttcgtggct gacgtgatcg accgctaccc gcgcagccgc ctcgagggca
     1081 cgcgctggat cctggtcgag gcccaggacc ggatcatgat ggagatcgcg ccgagcctcg
     1141 ccgagttcgc catgcgcgac ctgcgcggta ggggcatcga ggtgcgcacc gggacgcgcc
     1201 tcgacgcggt cgaagagtcg gccgttgtgc tctcgacagg cgagcgcatt ccgtgccgca
     1261 ccgtctgctg gacggccggc gtgaagccgc cggcgctcgt gcgcgacctg gggctgccgc
     1321 tcacccacga gggacggatc gacgtggacg agaacatgcg tgtgcgcggt cgcgagaacg
     1381 tgtgggcgat cggcgactcc gccgcggtgc cggatccggc gaagtcgcgg actcagccca
     1441 gcccgcccac cgcgcagcac gcgctgcgcc aggggaagcg cgtcgccgag aacgttgcgg
     1501 cgacgctcgg gcacgggcat attcggccgt tcacctacaa gacgcttggt gtgttcgtgg
     1561 acatggggcg acacaaggcc gtcgccgagg tgcttggcgt gcggctgcgc gggttccccg
     1621 cctggtgggc ggcgcggacc taccacctcg cggcgatgcc gggcgtgggg cggcggctgc
     1681 gcctgctcgt cgactggacg gttgcgctgc tcttccagcg cgactccgcc gagctcggcc
     1741 agctcggcca tccgccatct ctgcgcggct acctcgagac gccctcgccg gagccgaagg
     1801 agggagagac cgcggtgcgc gaggccggca gcggctcctg atggtcgccg aggtgaccct
     1861 gcgcgggggc accaaccgcg atctcgaggc cacacacgca ctctcgcaac gggcgatctt
     1921 cgaagccgcc gtcaagcgtg gcaccctgcc gccggacacg aagccgaacc cggacgagac
     1981 gcgcgccgag tggaagcgcc agcgcgggct gatcgagtat ctcgctgcgc agcccagcgg
     2041 ccgctactgc atcgcggaga aggacggcga gctcgtgggg tacggccgcg tggtccgctt
     2101 cgaccgcgtg gagcagctca ccgagctgat ggtggagccc ggccaccagg gcgagggcat
     2161 tggtcgggcg atcctcgaat cctgctggcc gggcgacccg tcgccggatc tcggtcggct
     2221 cgtgatcgcg acaggcgaac cgaacgacct cggcctgtac atgagcttcg gcgtgatgcc
     2281 ggtctgcggg cattggcaga tgcggcaggg caccgcggcg ttcgtggagc gacgctcgca
     2341 agagctcgac cgccaggaga cgggcgtgca cgtgctgaag ccagaccgcg cgctcgcgga
     2401 gtggcggcgc atggagccgg gcgcgctcgg cttcagtcgg cccggcctgc acgagcactt
     2461 cgcgcgcgat cgcacctgcc tcgccacctt cgacgccgag gcatcgcgcg ccacctcgct
     2521 gtgctgggtc tcgagcgagg gggagatcgg cccggcggtg gccgagagct cgcaggccct
     2581 cgtgccggtc gtgctcgccg tgctcgaccg cgtggcggcc acgcaggagc cggagttcct
     2641 gagcgtgttc tccacgacgg tcagctgggc gctcttgcgc aggctgcgcg tcctcggctt
     2701 cgagatctac tggcccagct ggatcatgtc ttctaagccg ctgcccgggc tcgaccgcta
     2761 cgtgcccacg cggcctccgc acgtgctctg agtacgatca gagcatgcgt gaagcagctt
     2821 cggatttcgg ccgcaaggca ctcgcggtgc ttgtgctgct gctggcggcc tggatcctct
     2881 tcaagatcgt cctcggcttc gccgcggtcc tcttctgggt cgtgctggcc gtgctcgctg
     2941 tcctcgcggt tctctgggcc gtgaaccaac tcctctgagg agagtcccgt gcagggcaca
     3001 gcgttcgtcg tgatcgcctt cttcttcggc ctctcggcgg gaacaatcgg caagatcaag
     3061 ggaagctcgt tcttcctgtg gttcgcgatc gggttcgtgc tcccgatcct cggcacgctg
     3121 gcggcgctgt tctaccgctt cgaccgccac gagccgaagc gccgctgccc ggagtgcggc
     3181 aacgtgctgc acgtgcacga ccaggtgtgc atgcgctgcg ggcgcgacct ggacttcccc
     3241 ggcgcggagg aggaggcaca gcccgccgcg cgtt
//
LOCUS       DASVXS010000302        11813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1270867, whole genome shotgun sequence.
ACCESSION   DASVXS010000302 DASVXS010000000
VERSION     DASVXS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270867"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /locus_tag="VF752_16915"
     CDS             complement(<1..2043)
                     /locus_tag="VF752_16915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8857278.1"
                     /translation="MVGATLVIGVLSVVGYILAIAGTAPDLSELKPIDKGSLTKVYAA
                     NGQVLGYVQSEQLRTPIPWSDMPKHLRNATVAIEDQRFYKHGGVDYESIVRAAFRNLK
                     SGKTVQGGSTITQQLVRNLYIKSPKRDFARKIREAKLASELEKKHSKQWVLENYLNDI
                     PYGTVNSRTAIGVEAAAETYFSKHAKDLTLAQAAMIAGLPQAPSEYNPFRNPSAALAR
                     RNQVLRKMAQMHYITQKQAQRAMRRSLALKQGTLYTKRREPYFFDFVQEQLIERYGVN
                     VLRQGGLKVYTTIYPSLQVAARNAMQNRLYLPTDPASAIVSIDPSNGYIRAMASSRAY
                     KSGTFNLASQGHRQPGSAFKTFVLTTAIKRGVNPNSTTYTSKELHLNTRYGPWNVKTY
                     SNSYLGRVNLVKATLASDNTVYAQLDLDLGPKSVAATAKSMGITTKLDGLPAEGLGGL
                     RLGVSPLEMADAYATLASGGVRHRPVAITKVEFPDGKSDNFAKPKGKQVLTDGQAYEV
                     TKILHQNVLGGTGKAANVGCPAAGKTGTTDNFNDAWFVGYTPHLATAVWVGYPNALVQ
                     MRSVHGISVAGGTFPAQIWHDFMNVAKGSNCDDFKQPSTPVQWAPFYGKYSSTGGGSG
                     RTGSYSSPGNGAAPPTNGGTTAPNTGGGNNYYDPRLYQTPPDVPQNTAPPGKKPKSN"
     gene            complement(2195..2983)
                     /locus_tag="VF752_16920"
     CDS             complement(2195..2983)
                     /locus_tag="VF752_16920"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX8857279.1"
                     /translation="MRLPDLPIASEFWDNNRQWLTALITFAVAILISQVVDRMLQRRG
                     KTIATAVTRGELSPVADTRLRLVRRLISVAILLIGLALALNQFSDLKRVATGILASSA
                     VIGIVVGFAARQTFANVIAGILLAITQPIRIGDLVTFGDQTGTVEDVRLSYTYMRAGD
                     GRRIIIPNELLAQGTIVNHTIFDPRVRVEVSVWIPIDADPDAAMAALSEEPGVEVALA
                     ETDKDGLRISVVSWAQSASDRDSIAAGLRLKCIERLREASILGT"
     gene            2982..4238
                     /locus_tag="VF752_16925"
     CDS             2982..4238
                     /locus_tag="VF752_16925"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX8857280.1"
                     /translation="MAQQAISKAGAADTTRGRAAARFPLQVFVGSRLLVFVVAVASAA
                     LLSDSPRRALFDVPRLTEPLGGFGRALLSPLAHWDAVWYLGIAEHGYTHAAGGGAGPD
                     AAFFPLYPLLVRLVAIEPTPGALLVSAYLVSLACFGAALVLLDRLVSLELGRDTARLT
                     LLLISLWPAALYFGAPYTESLFLLLTVAAFYAARTGHAASAGALAALASGTRPVGALL
                     AIPLALILWRYARSRRDLAWLALAPAGLAAYAAYLGVRFGDPLAFNSAQAFWFRGFTG
                     PWTGVKDGAVAAFEGARQLASGSRSPVYFTRAGGDPLIVARQNVLLFGALAAILVMLV
                     GAVRRLPPAWSVWALLSITLPLSSPVGPQPLMSLPRYAAVVFPLFAWLALVLRGRRGL
                     TIATLSVFAAGLVVATGYYATWHWVA"
     gene            4235..4906
                     /locus_tag="VF752_16930"
     CDS             4235..4906
                     /locus_tag="VF752_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8857281.1"
                     /translation="MKAVLLDSFGTLLRMDAPGPHLRRELAALGAEVSEERAAAAFRA
                     EIAYYLEHNLEGRDADSLLDLRSRCAAVVREAIGRPDLDLGSVREAMLRSIRFSAFAD
                     AAPALEDLRGRGLRAVVASNWDCSLPQALEHAGLRSLVDGVVASAVVGAAKPAPELFR
                     AALELVGAEPEDALHVGDSLEKDVEGARAAGLRAVLIARDGGAEPDRDTPVIRSLAEL
                     PALLT"
     gene            4913..5749
                     /locus_tag="VF752_16935"
     CDS             4913..5749
                     /locus_tag="VF752_16935"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX8857282.1"
                     /translation="MAVHPGSLPAPAGDSARPPQWPAWYAPVAFVVAFAASLFAFVVI
                     GAFGAALGANVDDPGPTLTVIGTVVQDVMLVVVAVFFAARVARPRLWQFGLRPARFWK
                     TVGLSLGALFTYYAFVGIYSALVKPHGEQTVTQDLGANNGTARMVLAGILVIVIAPVA
                     EEIFFRGFFYKALRSRFGIASAALIDGAVFGVIHYTGSGTLEILPILAALGFIFCLLY
                     ERTGTLFSTIALHAINNTIAYAASTDGATGFSIALGAVMVAACMIVPRYLGRGTPAAV
                     PT"
     gene            5746..6471
                     /locus_tag="VF752_16940"
     CDS             5746..6471
                     /locus_tag="VF752_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8857283.1"
                     /translation="MNAVEGRGLRKAYGAIVAVDGIDFDVRERECYGFLGPNGAGKTT
                     TMKMIYGLASVGAGSLEVLGMDVARQRREVKSRIGVVPQDANLDWELSARENLEIQAR
                     YHGVSPDGRIDELLRDAVLEQRADSKPRELSGGMQRRLLIARALVNNPQLIVLDEPTT
                     GLDPQARLAVWGRLERLKEQGVTLILTTHYMEEAERICDRLAIMDNGKIVAEGTPREL
                     CARYEQPNLEGVFLHITGHGLRE"
     gene            6468..7262
                     /locus_tag="VF752_16945"
     CDS             6468..7262
                     /locus_tag="VF752_16945"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8857284.1"
                     /translation="MTAIAACTLGSLPNPRMAYRVWRRNLLVFSKLWRGSLLPNFIDP
                     VFYLLAMGFGLGTYLQSVNGIPYKEFIASGLVASSVMWAASFETTWNIYFRMTENKLY
                     DAILATPVEVEDIVVAEVAWAATRAVIYGTAFLLIVALFGFVESPWAVFTPLFLALGG
                     ACFGMMGLAFTTAVNRIDLYSYYYTLLITPLFLFSGIFYPLDRLPEWTHVVAWFTPLY
                     HLVQITRGLILGPELLSVLGNAVWLLVLTSLLFAIPVRAMRKRLVA"
     gene            7333..7701
                     /locus_tag="VF752_16950"
     CDS             7333..7701
                     /locus_tag="VF752_16950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8857285.1"
                     /translation="MGQPVVHFEITGKDGAKLQSYFADLFGWEIDANNPMGYGVIQRE
                     GNTSPAGIGIGGGIATGPQGYPGHVTFYVEVPDVEASLSKAEQMGGKRMMGPEKVMEG
                     IEIGLFTDPEGHVIGLIKGQ"
     gene            7778..8740
                     /locus_tag="VF752_16955"
     CDS             7778..8740
                     /locus_tag="VF752_16955"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8857286.1"
                     /translation="MRRTGPLAFAALLSFAPSALADTSTQAAAHDFGTRVHVASGISD
                     GGKAYSLKGDAVTIEGPRPFAESGTATVEIFRGKRKLDTVKAKLRGPQGKRGGFSAKF
                     RARRLGDYKLRVTIRRDADDSSSRTAKLGVTVIAPVASAGSSGIRVRLLQRGLHNLGF
                     VTSMNGQYDAATQRAVLAFRKTNSMARTTSASKSVFGQLFAGKGGFKLRYPNAGKHVE
                     FDWSRQVLVLARGGKPERIYHASSGKPSTPTVFGTFHFYSKTPGTNAKGMVYSNYFIG
                     GYAIHGYAEVPPYAASHGCIRVPIPNAVSIYNWINLGDTIYVYR"
     gene            complement(8737..8982)
                     /locus_tag="VF752_16960"
     CDS             complement(8737..8982)
                     /locus_tag="VF752_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8857287.1"
                     /translation="MPAFVYLLRCADGSLYCGWTTDVERRTLAHQSGRGARYTRTRLP
                     VELVWSRAMPSRADAMREEARVKRLSRAAKLALAPAG"
     gene            complement(8988..9374)
                     /locus_tag="VF752_16965"
     CDS             complement(8988..9374)
                     /locus_tag="VF752_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857288.1"
                     /translation="MIQVLRAASTVAVLIVVASFTMFVVDEAGKGSDQQVRMVQNDIN
                     TTAPDATTEKRREAQHSAVREKIDDANDVLVKPFTGIVSGSGSVWVQRGVTTVLALLL
                     YGLLSRVLISYVAEAPSRARSAIRRT"
     gene            9579..9935
                     /locus_tag="VF752_16970"
     CDS             9579..9935
                     /locus_tag="VF752_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857289.1"
                     /translation="MSFLIDAPWLYAKGAAYARLAPEAAQGNVARALAATTVAGFWAV
                     SVSLYLNAKWTKPIWQASGAKSGRDFMINSGIFRLDHKRKDPAIHLVAGALFATYPLW
                     LLAGYRRGRRARLHRA"
     gene            9932..10483
                     /gene="pyrE"
                     /locus_tag="VF752_16975"
     CDS             9932..10483
                     /gene="pyrE"
                     /locus_tag="VF752_16975"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX8857290.1"
                     /translation="MSARERLIQALREHALVIGEVTLTSGRTAHYYVDAKRAILLPDG
                     FAAMGELVAEQAAALGATAVGGLTMGADPIACSALAAGADVKAFFVRKEVKPHGLSRP
                     IEGPPLDPSDRCLVVEDVVTTGGSTVAAIERIFEAGHEIVGVVSVLDRLAGGTDAIRE
                     AARGAPYTALTTINDVYPERPDR"
     gene            complement(10597..11640)
                     /locus_tag="VF752_16980"
     CDS             complement(10597..11640)
                     /locus_tag="VF752_16980"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX8857291.1"
                     /translation="MSGQTRLEDELRDQGAVLARRAQAGAEAAERAAALLRRDDVRYL
                     VVAARGSSDNAARFGQYLLGSAVRLNVALAAPSLYSDPARAPDLSGAAAMAISQSGQS
                     PDIVSVLTAARAQGRPTIAITNDENSPLALAAHAVVPLATGPEESLSATKTYVASLHS
                     LVQLAQALAPDDERASGLAALPALLTSAVEEQLASRSRFDVLRDATRLTAVGRGVSFA
                     TAYETALKIRELAGITAEAFSPPDLLHGPIAALTSGNWVWLAAGHDRQGQGAELLSKL
                     RQRSVPAVVVAQSTALLAAADVAIPLRGDVPDWLAAILAVVPGQVAGFHLAELRGVEI
                     DHPHGLSKVTLTH"
BASE COUNT         1646 a         4101 c         4120 g         1946 t
ORIGIN      
        1 gttcgacttg ggtttcttgc cgggcggcgc ggtgttctgc gggacatcgg gcggagtctg
       61 gtagaggcgc gggtcgtagt agttgttccc gccgccggtg ttcggcgccg tggtgccgcc
      121 gttcgtgggc ggcgccgcgc cgttgcccgg tgacgagtac gagcccgtcc taccggagcc
      181 gccgccggtc gaggagtact tgccgtagaa cggcgcccac tgcacgggcg tgctcggctg
      241 cttgaagtcg tcgcagttcg agcccttcgc cacgttcatg aagtcgtgcc agatctgcgc
      301 cgggaacgtg ccgccggcca cgctgatgcc gtgcacgctg cgcatctgga cgagcgcgtt
      361 cggatagccc acccacaccg ccgtcgcgag gtgaggcgtg tatccgacga accaggcgtc
      421 gttgaagttg tcggtggtgc ccgtcttgcc cgcggccggg cagccgacgt tcgccgcctt
      481 gccggtgccg cccaggacgt tctggtgaag gatcttcgtg acctcgtagg cctggccgtc
      541 ggtgagcacc tgcttgccct tgggcttggc gaagttgtcg gacttgccgt cggggaactc
      601 caccttcgtg atcgcgaccg gccggtgccg cacgccgccc gaggcgagcg tggcgtaggc
      661 gtcggccatc tcgagcggcg acacgccgag ccgcaggccg ccgagcccct cagcggggag
      721 cccgtcgagc ttcgtggtga tgcccatcga cttggccgtc gccgcgaccg acttcggccc
      781 gagatcgaga tcgagctggg cgtacacggt gttgtccgag gcgagcgtgg ccttcaccag
      841 gttcacccgc cccaggtagc tgttgctgta ggtcttcacg ttccacggcc cgtagcgcgt
      901 gttcagatgc agctcctttg acgtgtacgt cgtgctgttc gggttgacgc cgcgtttgat
      961 ggcggtcgtg agcacgaacg tcttgaatgc cgagcccggc tggcggtggc cctgcgaggc
     1021 gaggttgaaa gtgcccgact tgtaggcgcg gctcgaggcc atcgcgcgga tgtagccgtt
     1081 cgagggatcg atcgacacga ttgccgaggc cgggtcggtc ggcaggtaca gccggttctg
     1141 catcgcgttg cgcgcggcca cctgcaggct tgggtagatc gtggtgtaga ccttcagccc
     1201 gccctggcgc agcacgttca cgccgtagcg ctcgatcaac tgctcctgca cgaagtcgaa
     1261 gaagtagggc tcgcgccgct tcgtgtagag cgtgccctgc ttgagcgcga gcgagcgcct
     1321 catcgcccgc tgcgcctgct tctgcgtgat gtagtgcatc tgggccatct tgcggaggac
     1381 ctgattgcgc cgggcaagcg cggccgacgg gttgcggaac gggttgtatt ccgagggtgc
     1441 ctgcggcagg ccggcgatca tcgccgcctg cgcgagggtg aggtccttcg cgtgcttcga
     1501 gaagtaggtc tcggccgcgg cctctacgcc aatagccgtg cggctgttca cggtcccgta
     1561 cgggatgtcg ttcaggtagt tctcgagcac ccactgcttc gagtgcttct tctcgagctc
     1621 cgaggcgagt ttggcctcac ggatcttgcg ggcgaagtcg cgcttcgggc tcttgatgta
     1681 gaggttgcgg acgagctgct gcgtgatcgt cgagccgccc tgcacggtct tgccactctt
     1741 gaggttgcgg aacgccgcgc gcacgatcga ctcgtagtcc acgccgccgt gcttgtagaa
     1801 gcgctggtcc tcgatcgcca cggtcgcgtt gcggaggtgc ttcggcatgt cgctccacgg
     1861 aatcggcgtg cggagctgct cggactgcac gtagccgagc acttgcccgt tagcggcgta
     1921 gaccttggtg agcgagccct tgtcgatcgg cttgagctcc gacaggtcgg gcgccgtgcc
     1981 cgcgatcgcg aggatgtagc ccacaaccga gagcacgccg atcaccagag ttgccccgac
     2041 cacgcccagc cctaggagca gccagttacg gggacgtccg cgatggcgtt gccgtcgctg
     2101 ctttcgctgt cgataggtca agagcccgac cgcctgggtt cagctacgcg ccccggcccg
     2161 gggagccgaa tcggtgccct cggcgggcgc gcgatcacgt tccgaggata gaggcttcgc
     2221 gcaatcgttc gatgcacttg aggcgaagtc ctgccgcgat gctgtcccgg tctgaggcgc
     2281 tctgggccca gctgacgacg ctgattcgca gtccgtcctt gtcggtctcg gcgagcgcca
     2341 cctctacccc gggctcctcc gagagagcag ccatcgctgc gtcggggtcg gcgtcgatcg
     2401 gaatccacac cgacacctcc acccgcacgc gcgggtcgaa gatcgtgtga ttcacgatcg
     2461 tgccctgcgc caagagctcg ttcgggatga tgatccggcg cccgtcgccc gcgcgcatgt
     2521 acgtgtatga gagacgcacg tcctcgactg tgccggtctg gtcgccgaac gtgacgaggt
     2581 caccgatccg gatcggctgc gtgatcgcga gcaggatgcc ggcgatcacg ttggcgaagg
     2641 tctggcgcgc cgcgaagccg acgacgatgc cgatcaccgc ggacgacgcg agaatgcccg
     2701 tcgcgacccg cttgaggtca ctgaactgat tcagcgccag tgccaggccg atcagcagga
     2761 tggctacgct gatcagccgg cgcaccagcc gcagccgcgt gtccgcgacc ggcgagagct
     2821 cgccacgcgt gacagcggtg gcgatcgtct tgccgcgccg ctgcagcatg cggtccacga
     2881 cctgcgagat cagaatggcg accgcgaagg tgatcagcgc ggtgagccac tggcggttgt
     2941 tgtcccagaa ctcgctcgcg atgggaagat ccggcagacg catggcgcag caggctatct
     3001 ccaaggcggg ggcagctgac accacccgcg gccgcgcggc cgctcgcttc ccgctccagg
     3061 tgttcgtggg ctcgcggctg ctcgtgttcg tcgtcgccgt cgcgtcggcc gcactgctct
     3121 ccgactcgcc gcggcgcgcg ctgttcgacg tgccacgact caccgagccg ctcgggggct
     3181 tcgggcgtgc gctgctgtcg ccgctcgccc actgggacgc cgtctggtac ctgggcatcg
     3241 ccgagcacgg ctacacgcac gccgcgggcg gcggagccgg cccggacgcg gccttcttcc
     3301 cgctctaccc gctgctcgtg cgcctcgtgg cgatcgagcc cacgccgggc gcgctgctcg
     3361 tgtcggccta tctcgtgtcg ctggcgtgct tcggcgcggc gctcgtgctg ctcgaccgcc
     3421 tggtgtcgct cgagctcggg cgcgacaccg cgcgcctcac gctgctgctc atctcgctct
     3481 ggccggccgc gctctacttc ggcgccccct ataccgagag cctcttcctg ctcctcaccg
     3541 tggcggcgtt ctacgcggcg cgcaccggcc acgcggcctc ggccggagct ctcgcggcgc
     3601 tcgcgtccgg cacccgcccc gtcggcgccc tgctggccat cccgctcgcg ctgatcctct
     3661 ggcggtacgc gcgctcgcgg cgcgacctcg cctggcttgc gctcgcgccc gcgggactcg
     3721 cggcctacgc cgcctacctc ggcgtccgct tcggcgatcc gctcgcgttc aacagcgcgc
     3781 aggcgttctg gttccgtggc ttcaccggcc cgtggaccgg cgttaaggac ggcgcggtgg
     3841 cagccttcga gggcgcgcgc cagctcgcgt cggggtcccg ttcgcctgtg tacttcacgc
     3901 gcgcgggcgg cgacccgctg atcgtcgcgc gccagaacgt actgctcttc ggcgcgctcg
     3961 cagcgatcct ggtgatgctg gtgggggcgg tgcggcggct gccgccggcg tggagcgtgt
     4021 gggccctcct gtcgatcacg ctgccgctgt cgtcgccggt cggcccgcag ccgctgatgt
     4081 cgctgccgcg ctacgcggcc gtcgtgttcc cactgttcgc ctggctcgcg ctggtgctgc
     4141 gcggacggcg cgggctcacg atcgcaacgc tgtcggtatt cgccgccggc ctggtggtcg
     4201 ccaccggcta ctacgcgacc tggcactggg tcgcgtgaag gcggtcctgc tcgactcctt
     4261 tggcacgctc ctgcggatgg acgcgcccgg cccgcacctg cggcgcgaac tggcggcgct
     4321 cggcgccgag gtcagcgagg agcgggccgc ggcggcgttc cgcgcggaga tcgcctacta
     4381 cctcgagcac aacctcgagg ggcgggatgc ggattcgctg ctcgacctgc gcagccgctg
     4441 cgccgccgtg gtgcgcgagg cgatcggccg gccggacctc gacctcggct cggtgcgtga
     4501 ggcgatgctg cgctcgatcc gcttcagcgc gttcgcggac gccgcgccgg ctctcgaaga
     4561 tctgcgcggt cgggggctta gggccgtggt cgcgagcaac tgggactgct cgctgccgca
     4621 ggccctcgaa cacgcgggcc tgcgctcgct cgtcgacggc gtcgtggcca gcgcggtggt
     4681 gggcgccgcc aagccggcgc ccgagctctt ccgcgcggcg ctcgagctcg tgggagccga
     4741 gccggaggac gcactgcacg tgggcgactc gctcgagaag gacgtcgagg gagcgcgcgc
     4801 ggccggcctg cgagcggtgc tgatcgcccg cgacggaggc gcggagccgg atcgcgacac
     4861 ccccgtgatc cgcagcctcg cggagctgcc cgccctccta acctaagctg gcatggccgt
     4921 tcaccccgga tcgctgccgg cgccggccgg cgacagcgcc cgcccgccgc aatggccggc
     4981 ctggtacgcc ccggtcgcat tcgtcgtcgc cttcgccgcg tcgctattcg cgttcgtggt
     5041 gatcggagct tttggcgccg cgctcggcgc gaacgtcgac gacccggggc cgactctcac
     5101 cgtcatcggg accgtcgtgc aggacgtgat gctcgtggtc gtcgcggtct tcttcgccgc
     5161 gcgcgtcgct cgcccgcgcc tctggcagtt cgggctgcgg cccgcgcgct tctggaagac
     5221 ggtcgggctg tcgctaggcg cgctgttcac ctactacgcg ttcgtcggga tctacagcgc
     5281 gctcgtgaag ccccacggcg aacagaccgt cacgcaggac ctcggcgcga acaacggcac
     5341 agcccgcatg gtgctcgcgg ggatcttggt gatcgtgatc gcgccggtgg ccgaggagat
     5401 cttcttccgc ggcttcttct acaaggcgct tcgctcgcgc ttcggcatcg cctcggcggc
     5461 gctgatcgac ggcgccgtct tcggcgtgat ccactacacc ggctccggca cgctcgagat
     5521 ccttccgata ctcgcggcgc tcgggttcat cttctgcctg ctctacgagc gcaccggcac
     5581 gctcttcagc acgatcgccc tgcacgcgat caacaacacg atcgcctacg ccgcgtcgac
     5641 cgacggcgcg actggcttct cgatcgcgct gggagcggtg atggtggccg cctgcatgat
     5701 cgtcccgcgc tacctcggcc gcggcacgcc cgccgcggtg cccacgtgaa cgccgtagag
     5761 ggccgcgggc tgcgaaaggc ctatggggcg atcgtcgcgg tcgacggcat cgacttcgat
     5821 gtgcgcgagc gcgagtgcta cggcttcctc ggtccgaacg gcgccggcaa gacaacgacg
     5881 atgaagatga tctacgggct cgcgagcgtt ggcgccggct cgctcgaggt gctcggcatg
     5941 gacgtcgcac ggcagcgccg cgaggtgaag tcgcggatcg gcgtcgtgcc ccaggacgcc
     6001 aacctcgact gggagctgtc cgcgcgcgag aacctcgaga tccaggcgcg ctaccacggc
     6061 gtgagccccg acgggcggat cgacgagctg ctgcgcgacg cggtgctcga gcagcgcgcc
     6121 gactcgaagc cgcgtgagct gtccggcggc atgcagcgcc gcctgttgat cgcgcgcgcc
     6181 ctcgtgaaca acccgcagct gatcgtgctc gatgagccca ccaccggact cgacccgcag
     6241 gcgcgcctgg cggtgtgggg ccggctcgag cgtctcaagg agcagggcgt gacgctgatc
     6301 ctcacgacgc actacatgga agaggccgag cgcatctgcg accgcctggc gatcatggac
     6361 aacggcaaga tcgtggccga gggcacgccg cgcgagctgt gtgcgcgcta cgagcagccg
     6421 aacctggagg gcgtgttcct ccacatcacg gggcacgggc tgcgcgagtg accgccatcg
     6481 ccgcatgcac cctcggcagc ctgccgaacc cgcgcatggc ctatcgcgtc tggcggcgca
     6541 acctgctcgt gttctcgaag ctctggcgcg ggtcgctgct cccgaacttc atcgatcccg
     6601 tcttctacct gctggcgatg ggcttcggcc tcggcacgta cctgcagtcg gtgaacggca
     6661 tcccgtacaa ggagttcatc gcgtcggggc tcgtggcgtc gtcggtgatg tgggcggcgt
     6721 cgttcgagac cacctggaac atctacttcc ggatgaccga gaacaagctg tacgacgcga
     6781 tcctcgcgac gccggtcgag gtcgaggaca tcgtcgtggc ggaggtggct tgggccgcca
     6841 cgcgcgcagt catctacggg acggcgttcc tgctgatcgt cgcgttgttc ggcttcgtgg
     6901 agtcgccgtg ggcggtgttc actccgctct tcctcgcgct cggcggcgcc tgcttcggga
     6961 tgatggggct cgcgttcacc acggccgtga acaggatcga tctctactcg tactactaca
     7021 cgctcctgat cacgccgctc ttcctcttca gcggcatctt ctatccgctc gatcgcctgc
     7081 ccgagtggac gcacgtggtc gcgtggttca cgccgctcta tcacctggtg cagatcaccc
     7141 gcgggctcat cctcgggccg gagctgctct ccgttctcgg caacgcggtg tggctgctcg
     7201 tgctcacgtc gctactgttc gcgatcccgg tgcgcgcgat gcgcaagcgg ctcgtggctt
     7261 agaggccgat gaatccctgg tcgtccggtc gtctccctga taggcctagg acaccagcca
     7321 aaggagagga cgatgggaca gccggtagtg cacttcgaga tcaccggaaa ggacggcgcc
     7381 aagctgcaga gctatttcgc cgacctgttc ggatgggaga tcgatgccaa caacccgatg
     7441 ggctacggcg tgatccagcg tgagggcaac accagccccg caggcatcgg catcggaggc
     7501 gggatcgcga ccgggccaca gggctatccc ggccacgtga ccttctatgt cgaggtgccg
     7561 gacgtcgagg cgtccctcag caaggccgag cagatgggcg gcaagcgcat gatgggcccc
     7621 gagaaggtga tggaggggat cgagataggc ctcttcaccg accccgaggg ccacgtgatc
     7681 gggctcatca agggccagta gcggtcccga gagcgcgctg cgggggtgcg gatagccgta
     7741 aagctacgtc cgctcagggc gattagagtg accgttgatg cgccgcaccg gtcccctcgc
     7801 cttcgcggcg cttctttcgt ttgccccttc cgcgctcgcc gacacgtcca ctcaggcggc
     7861 ggctcacgac ttcggcacgc gcgtgcacgt tgcctccggc atcagcgacg gcggcaaggc
     7921 gtactcgctg aagggcgacg cggtcaccat cgaggggccg cgcccgttcg ccgagagcgg
     7981 cacggccacg gtcgagatct tccgcggcaa gcgcaagctc gacacggtca aagcgaagct
     8041 ccgggggccg cagggcaagc gcggcggctt cagcgcgaag ttccgcgccc gccgtctcgg
     8101 ggactacaag ctgcgcgtca ccatccgccg cgacgctgac gacagctcga gccgcacggc
     8161 caagctgggc gtgacggtga tcgcgccggt cgcgagcgcg ggctcgagcg ggatccgcgt
     8221 gcggctgctc cagcgcggct tgcacaacct cggcttcgtc acctcgatga acggccagta
     8281 cgacgccgcc acccagcgcg cggtgctcgc gttccggaag acgaacagca tggcccgcac
     8341 cacgagcgcg agcaagtcgg tgttcgggca gctgttcgcc ggcaagggcg gcttcaagct
     8401 gcgctacccg aatgccggca agcacgtgga gttcgactgg tcgcgccagg tgctggtgct
     8461 cgcgcgcggc ggcaagccgg agcgcatcta ccacgcctcc tcgggcaagc cgtccacgcc
     8521 caccgtcttc ggcaccttcc acttctactc gaagacgccc ggaacgaacg ccaagggcat
     8581 ggtctactcg aattacttca tcggcggcta tgcgatccac ggctacgcgg aggtgccgcc
     8641 gtacgcggcg agccacggct gcatccgggt gccgatcccg aacgccgtgt cgatctacaa
     8701 ctggatcaac ctcggcgaca cgatctacgt gtaccgctag ccggccgggg cgagcgccag
     8761 cttcgccgcg cggctgagcc gcttcacccg cgcctcttcg cgcatcgcgt ccgcgcgcga
     8821 gggcattgcg cgcgaccaca cgagctcgac cgggaggcgc gtgcgcgtgt agcgcgcgcc
     8881 ccgtcccgac tgatgcgcga gcgtgcgccg ctcgacgtcg gtggtccagc cgcaataaag
     8941 cgagccgtcc gcgcagcgca gcaggtacac gaacgccggc acgcggccta ggtgcgcctg
     9001 atcgccgagc gcgcgcgcga cggcgcctcg gcgacgtacg agatcaacac gcgcgaaagc
     9061 agtccgtaga gcaggagcgc aagtacggtg gtcactccgc gttgcaccca gaccgagccc
     9121 gaaccgctca cgatcccggt gaacggcttc acaaggacgt cgttggcgtc gtcgatcttc
     9181 tcgcgcacgg cgctgtgctg ggcctcgcgc cgcttctcgg tggtggcgtc tggcgcggtc
     9241 gtgttgatgt cgttctggac catccgcacc tgttggtccg agcccttgcc agcctcgtcg
     9301 accacgaaca tcgtgaagct cgccaccacg atcagcacgg cgaccgtcga ggccgctctc
     9361 agcacctgga tcacttcgcc accatcccga tcaccacgtc taggacttct tttcgatcag
     9421 cgcatgcgca agaaacacat ctacgacccc tccatcgaca gaaaggctgt tcggcttgat
     9481 tcgccgctga agtgctcgat ccgcccggga gggacgtcgt cggcgaccgc gtgcaatgga
     9541 acttgcgtcc gccgagccgg gctaggatac gcgcgctcgt gtccttcctg atcgacgctc
     9601 cgtggctcta cgcgaagggc gcggcctatg cgcggctagc gcccgaggcc gcgcagggga
     9661 acgtcgcgcg tgcgctcgcg gctacgaccg tggcgggctt ctgggccgtg tcggtgtcgc
     9721 tctacctgaa tgccaagtgg acgaagccga tctggcaggc gtccggcgcg aagagcgggc
     9781 gcgacttcat gatcaactcc ggcatcttcc gcctcgacca caagcgcaag gacccagcga
     9841 tccatctcgt cgcaggcgcg ctgttcgcga cctatccgct gtggctgctc gccgggtacc
     9901 gccgcggccg gcgggcgcga ctgcaccgcg cgtgagcgct cgcgagcgac tgatccaggc
     9961 gctgcgggag cacgccctcg tgatcggcga ggtgacgctc acgagcggcc gcaccgcgca
    10021 ctactacgtg gacgcaaagc gggcgattct gctgcccgac gggttcgcgg cgatgggcga
    10081 gctggttgcc gagcaggcgg ccgccctcgg tgccaccgcc gtgggtggct tgacgatggg
    10141 cgccgacccg atcgcctgct cggcgctcgc cgccggtgct gacgtgaagg ccttcttcgt
    10201 gcgcaaggag gtcaagccgc acgggctgtc gcggccgatc gagggcccgc cgctcgaccc
    10261 ttccgaccgc tgcctggtgg tcgaggacgt ggtcacgaca ggcggctcca cggtcgccgc
    10321 gatcgagcgc atcttcgagg cgggccacga gatcgtcggc gtcgtcagcg tgctggaccg
    10381 gctggccggc gggaccgacg cgattcgcga ggccgcacgg ggcgcgccct acaccgcact
    10441 tacaacgatc aacgacgtct acccggagcg ccccgaccgc taggcagctg cgtcgggtgg
    10501 tcccggattc gagcccgagg gcgccccgaa cgaagaggat cacgagcggg atgcccgtga
    10561 agagcaggac ccagccgggc cagaaggtgc ccgcgcctag tgcgtgagcg tcaccttgct
    10621 gagcccgtgt gggtggtcga tctcgacgcc gcgcagctcg gcgagatgga agccggccac
    10681 ctgccccggc accaccgcga ggatcgcggc gagccagtcg ggcacgtcgc cccgcagcgg
    10741 gatcgcgacg tcggccgccg ccagcagcgc ggtcgactgc gcgaccacca ccgcgggcac
    10801 cgagcgttgc ctgagcttgg agagcagctc cgcgccctgg ccctggcgat cgtgtcccgc
    10861 ggcgagccac acccagttgc cgcttgtgag cgcggcgatc ggcccgtgca gcagatcagg
    10921 cggcgagaac gcctcggcgg ttatgcccgc gagctcgcgg atcttcaaag ccgtctcgta
    10981 ggcggtcgcg aagctcacgc cccggcccac cgcggtgagg cgggtggcgt cgcgcaggac
    11041 gtcgaagcgc gagcgcgatg cgagctgctc ctccacggcc gaggtcagca gcgccggcaa
    11101 cgcggcgagc ccgctggctc gctcgtcgtc gggcgcgagc gcctgtgcga gctgcacgag
    11161 cgagtgcaac gaggccacgt acgtcttcgt agccgaaagc gactcctccg gcccggtcgc
    11221 gaggggcacc accgcgtggg ccgcaagtgc gagcggagag ttctcgtcgt tggtgatcgc
    11281 gatcgtcggc ctgccctgcg cgcgggcggc ggtgagcacg ctcacgatgt cgggcgactg
    11341 gccggactgc gagatcgcca ttgcggcggc cccgctcagg tcgggggcgc gtgccgggtc
    11401 ggagtacagc gacggggccg ccagcgccac gttcagacgc acggccgagc cgagcaggta
    11461 ctggccgaag cgcgccgcgt tgtcagagct gccacgcgcg gccaccacga ggtagcgcac
    11521 gtcgtcgcgg cgcagcagag ccgcggcccg ctcggccgcc tcggctcccg cctgcgcccg
    11581 gcgcgcgagc acggcaccct ggtcgcgcag ctcatcctcg agccgcgtct gcccactcat
    11641 gcgccgcacc ctaagcgcgc cggggttgtg gccgcaaggg ttgcgtggca tgcaggcccc
    11701 tgatcgcggg tatggctgcc ggcatgggca agcagggacg tggcctgtgg cgcgtgagcg
    11761 cgttgccgat cgtgctgacg ctcgctgcga tcggctgcgg cggcggagcg gga
//
LOCUS       DASVXS010000303         8361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_848117, whole genome shotgun sequence.
ACCESSION   DASVXS010000303 DASVXS010000000
VERSION     DASVXS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848117"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..876)
                     /locus_tag="VF752_16985"
     CDS             complement(7..876)
                     /locus_tag="VF752_16985"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX8857292.1"
                     /translation="MKSRALSTFSSDMPRHPFSRRERVAQTDELPHRRRSVFVDADKR
                     GRTMAIAFPGESAEYRAARDRLLEQEVALRRATEEVAAARRELPPGGVVPEDYVFQEA
                     GEGGNATDVRLSELFAPGKDSLAIYSFMFPRDPSDPRHGPETGQSALLPLIEGPCPSC
                     TAMLDQLDGAAEHVSQRLNFAVTANAPLSRVITFANERGWRRLRLLSSAANSYSRDYH
                     GETADGSPRPMLNVFHREGGTIRHFWGSELLYAPSEPGQDPRHVGTLEPLWNFFDLTP
                     EGRPSDWEEQLSY"
     gene            839..1237
                     /locus_tag="VF752_16990"
     CDS             839..1237
                     /locus_tag="VF752_16990"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8857293.1"
                     /translation="MSEENVDKARDFIAAYNRRDFDAAVESFDPEIEWVLPERQRSDS
                     CRGPDEIKRFWEGLDDTFDELRLEPQEFRDAGDHVATRLRYYSRAKSGVELETELYHQ
                     VATFRDGTIVRMEYFAEWSEALEAAGLGER"
     gene            complement(1277..1573)
                     /locus_tag="VF752_16995"
     CDS             complement(1277..1573)
                     /locus_tag="VF752_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857294.1"
                     /translation="MAVVLVHQGPTLTRESYDEVVRRMTDGQKDRMESPSDWPVEGLL
                     VHVAGEAPGGFRVLDVWESEEACNRFGEILGSRLQEVGITEQPELYQPHTFVSA"
     gene            1786..2238
                     /locus_tag="VF752_17000"
     CDS             1786..2238
                     /locus_tag="VF752_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8857295.1"
                     /translation="MGSRVRRAALERTVRAGYEAWNRHDMTVARAYADSDVEVHVAQG
                     ADLPVGLDDAYYGVDGYCRAMEEWAAAWRKWRVEVEKVVEVAQDRFLVTGRHFGEGRA
                     SGANLEQWSAALYTMRRGKIARVDMFFLADEESVSETVTSLAEGRARP"
     gene            complement(2317..3006)
                     /locus_tag="VF752_17005"
     CDS             complement(2317..3006)
                     /locus_tag="VF752_17005"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8857296.1"
                     /translation="MPTAIRAVARHRQTQVEAVARRARELGLLGWVRLDADAGLRLHA
                     EGDPGAIQALLRELGSVESEPARVEGHEQFAIRGVPAGVFVVQEHQATAHHFDLRLEV
                     DGVMRSWAVPKGPSMDPAVKRLAVQVEDHARSHNEYEGRGVIVWDRGSYEQGGRVAWP
                     EALERGHAVFVLHGEKLQGGFALQRTRDGKKPQWLLIKRSDEHARPGSDVVSERPESV
                     RSGRTLEQTLA"
     gene            3286..3561
                     /locus_tag="VF752_17010"
     CDS             3286..3561
                     /locus_tag="VF752_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857297.1"
                     /translation="MWDQVTHADVVRAIEEYDQLGPERFFSEHGFGPTTTYDLVWNER
                     LYPPKAVLGTAYEFATGQRLASGDFEGGKSGAVKVLGNLGFTIQPRR"
     gene            4003..4512
                     /locus_tag="VF752_17015"
     CDS             4003..4512
                     /locus_tag="VF752_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857298.1"
                     /translation="MSPSAPREDLPQFLPPMLASTGPAPDGEGWVHEVKWDGMRAQVR
                     IDGQVTVRSRPGRDCSREFPELAELAGHPTIVPGSRSPSRDSSRTFSSKSKSSPAIAF
                     TVAAKRRASIGSLITTRPSEPSFGTSRTRVASVSRSRGRGFGPCPITKVPPDRPPHPR
                     AIAAVMRSA"
     gene            4604..5539
                     /locus_tag="VF752_17020"
     CDS             4604..5539
                     /locus_tag="VF752_17020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8857299.1"
                     /translation="MPLRVGQPAPDHSVQAYVRGERAPAEFALSSHRGRWLVLFFYPR
                     DFTFICPTELASFAERHDDFLGERAVVLAASTDSYFSHKAWFESDPRLAGIRYPVIAD
                     SAHELSRSFDVLLEDGATHRGTFIIDPSGVLVHMSVNEHDVGRNVDEVLRTLRAFRTG
                     ALCPANWGPGKATLTSEDDWLEKVFPSLTGPALDSLAERAEKVSVVAGDTIVAEGDPA
                     DRFYVIAKGEVAVTRRGPEGQEIELATLGPGQFFGEVGLLAETRRTATVRAVADVELL
                     ALSWQEFQAALEQSDRAKRDFSEIVHERLAGASAS"
     gene            5536..5883
                     /locus_tag="VF752_17025"
     CDS             5536..5883
                     /locus_tag="VF752_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857300.1"
                     /translation="MSATSVLYVDDETVDRVIGARHGALVLAKDDCDNCAAYETQIRR
                     LHEEGQFGDLVIGKIVLTQPGCRDFKRANPWLGGVDFLPYTLLYASGEKVDEFAGSTG
                     TYLLERAADVGFI"
     gene            5979..6695
                     /locus_tag="VF752_17030"
     CDS             5979..6695
                     /locus_tag="VF752_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX8857301.1"
                     /translation="MKAVEAVLEDARDRDLIARNAAANKKLQLTAETPDRSYLHPVQI
                     EARFEAARQIEQETRGLSPADVAAIRASSASAVSLARDFGVSDVLIGKLRRGEVTEVG
                     RYGYVPRLALVATLVLGGQRIRELGLLDHHDINLADGYIRRPRLKTDASERSIPMLPA
                     LRDVLLEYRATQPPHVPPFATRTGKRFTGDNVRARLRPVRARANALLVARGHEPIARL
                     TPHTFRRTFASILAAAYPRD"
     gene            6709..6921
                     /locus_tag="VF752_17035"
     CDS             6709..6921
                     /locus_tag="VF752_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857302.1"
                     /translation="MGHEDATFTARVYRQVMKMHSGGLEGLERVIGCSRREGHFLLGG
                     YDQDPFWTETGPKDAKKPSAEGLAVS"
     gene            complement(6950..7023)
                     /locus_tag="VF752_17040"
     tRNA            complement(6950..7023)
                     /locus_tag="VF752_17040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6987..6989),aa:Gly,seq:gcc)
     gene            7409..>8361
                     /locus_tag="VF752_17045"
     CDS             7409..>8361
                     /locus_tag="VF752_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857303.1"
                     /translation="MRRWTRESADARGRLLEAGEALAAARNEAQRYQVDAARLGEERE
                     QIAGQLADAREALATAATEAERQAEEHGALERRVEELQRERDEARGDAERIAAELTAA
                     RREAEEAASAAHEQLQARTELEQRMERVQAEVEDRAAVLAEAERQVDTALRRRMDSFV
                     EAEREARAAQERDTAEAAARTAELDALAARIVRAREVLAELREESRREQDRHARLDDR
                     LRDIIGPEPEPAEQEAQRVTEREEPPAEHEPWVGNGESTDAAEAQRAGRRKLFGRRKQ
                     GPFIGRPGYCSVCSRELIVESKEELAASGWLISDGEAVCLS"
BASE COUNT         1377 a         2794 c         2921 g         1269 t
ORIGIN      
        1 gagggctcag tagctcagct gctcttccca gtcgctcggt cgcccctccg gagtcaggtc
       61 gaagaagttc cagagcggct cgagcgtgcc gacgtgtcgg gggtcctggc cgggctcgct
      121 cggggcatag agcagttccg agccccagaa gtggcggatg gttccgccct cgcggtggaa
      181 gacgttcagc atcggccgag gcgatccgtc cgcagtctcg ccgtggtagt cgcggctgta
      241 gctgttggcg gccgaggaca gcagccggag ccggcgccaa ccgcgctcgt tcgcgaacgt
      301 gatcacgcgc gacaacggcg cgttcgcagt gacggcgaag ttgaggcgct ggctgacatg
      361 ctcggcggcg ccgtctagct ggtcgagcat cgccgtgcac gacgggcacg gtccctcaat
      421 gagaggcagc agcgccgact ggccggtttc gggtccgtgt cgcggatcac tcgggtcgcg
      481 cgggaacatg aagctgtaga tcgcgagcga gtccttgccg ggcgcgaaca gctccgagag
      541 cctcacgtcc gtggcgtttc cgccctcgcc cgcttcctgg aagacgtagt cctcggggac
      601 gacgcctccg ggcggaagct cgcgccgcgc cgcggcaacc tcctccgtgg cgcgccgcag
      661 cgctacctcc tgctcgagca agcgatcacg cgctgcgcgg tactcggctg actctcctgg
      721 aaacgcaatc gccatggttc ttcctctctt gtcggcgtct acgaagacag accgccgacg
      781 atgcggcaac tcatcggttt gcgcaacgcg ctcgcggcgc gagaatgggt gccgcggcat
      841 gtccgaggag aacgtcgaca aggcgcgcga cttcatagcg gcttacaacc ggcgtgactt
      901 cgacgccgcc gtggagtcgt tcgatcccga gatcgagtgg gtgcttcccg agcgccagag
      961 atcggactcg tgtcgggggc ccgacgagat caagcgcttc tgggagggac tcgacgacac
     1021 cttcgacgag ctgaggctgg agccgcaaga gttccgcgat gccggggacc atgtagcgac
     1081 ccggcttcgc tactactcgc gagccaagag cggggtcgag ctcgagacgg agctgtacca
     1141 ccaagtcgcg accttccgcg acggaacgat cgtgcggatg gagtacttcg ccgaatggtc
     1201 ggaggcgctc gaagccgccg ggctggggga gagatagtcc accgggcgat cggggacccg
     1261 gcgtaaatgc gcgtcctcag gcggagacga acgtgtgcgg ctggtagagc tcgggctgct
     1321 cagtgatgcc gacctcttgc agacgcgagc cgagtatctc gccgaacctg ttgcacgcct
     1381 cctccgactc ccagacatcg agcacgcgaa agccgcccgg tgcttctcca gcgacgtgca
     1441 cgagaagccc ctcgaccggc cagtcggagg gcgattccat gcggtccttc tgcccgtcgg
     1501 tcatgcggcg gacgacctcg tcgtagctct cccgggtgag ggtaggtcct tggtgtacca
     1561 gtacgactgc catgtctgcc tcctcctggc gacgccgttg cggttgtgct cgtgataccg
     1621 ggagcaggcc cggcgatcac gtcaggctgc ttaggatcca catgtaggag tgatcgagtg
     1681 gccgccccac gaagcacggc gcgcgggcgc aacgtcgaac aacgagtagc tgtccgctgg
     1741 cccggtctgg cgcacttgac aggcgcgtcg ttaatgcgtc tcccggtggg atcgcgggtg
     1801 cggcgagcgg ccctcgagcg caccgtgcgt gccggttacg aggcatggaa ccgccacgac
     1861 atgacggtgg ccagggctta tgcggactcg gacgtcgagg tgcatgtcgc gcagggcgcg
     1921 gatttgccgg tgggattgga cgatgcctat tacggagtcg acggctattg ccgggcgatg
     1981 gaggagtggg ccgcggcctg gcggaaatgg cgcgtcgagg tagaaaaagt cgtcgaggtc
     2041 gcccaggaca ggttcctggt caccggccgc cactttggcg agggccgggc gagcggagcg
     2101 aacctcgagc aatggagcgc ggccctgtac acgatgcggc gggggaagat cgcccgagtc
     2161 gacatgttct tcctcgctga cgaggagagc gtgtcggaga cagtcacgtc gctcgccgag
     2221 ggcagagccc gcccatagct gcgcgcgggc gcgagtgcgc gagggggaag aagcctcaga
     2281 gagcggatga agggactcga accctcgccc cgcggcttag gcgagcgtct gctcgagcgt
     2341 ccgcccgctg cgcaccgact caggccgctc cgacacgacg tctgagccgg gacgcgcgtg
     2401 ctcgtcgctg cgcttgatca gcagccactg cggcttcttg ccgtcgcgcg tgcgctgcag
     2461 tgcgaaaccg ccctgcaact tctcgccgtg cagcacgaag accgcgtgcc cgcgttcgag
     2521 cgcctccggc catgcgacgc ggccgccctg ctcgtaactg ccacggtccc agacgatcac
     2581 gccgcgaccc tcgtattcgt tgtgcgagcg cgcgtggtcc tcgacctgca cggcgaggcg
     2641 cttgaccgcc gggtccatcg acggcccctt gggcacggcc cacgaccgca tcacgccatc
     2701 aacctccagg cgcaggtcga agtggtgggc agtggcctgg tgctcttgca cgacgaacac
     2761 cccggccgga acgccgcgga tcgcgaactg ctcgtggccc tccacacgcg cgggctcgct
     2821 ctccacgctg ccgagctcgc gtagcaacgc ctggatggca cccgggtcgc cctccgcgtg
     2881 cagccgtagt cccgcatcgg catccaatcg cacccagccc agcagaccga gctcgcgagc
     2941 acgccgcgct accgcctcaa cttgcgtctg gcggtgccga gcgacggcgc ggatcgcggt
     3001 gggcatggcg ccagttcgta acgcggctcg tcggcgccgt caaccgtttc gcgggcatac
     3061 gcgcgttgta ccgtggtcga ttttgccgcc atatgcggca ctaagcacca cggttcggag
     3121 gagcgccact gagcaacagc ggctagcgat cttgccgtga gcggaagcac ctgaaaagct
     3181 cggcacgccc gtcgcgggcg caaccggcgc acgggccacg cccccgatct acggcggcaa
     3241 tgcaggcgac aacgatcctc ggagaacgag atgacacatt cggtcgtgtg ggaccaggtg
     3301 acgcacgctg atgtggtgcg cgcgatagag gagtacgacc aactagggcc ggagcggttc
     3361 ttctctgagc atggattcgg cccaaccacg acttacgacc tggtctggaa cgaacgcctc
     3421 tacccaccga aagcagtctt gggaacggct tacgagttcg ccacgggcca gcgccttgcc
     3481 tccggtgatt tcgagggcgg gaagagcggc gccgtcaagg tgctcggaaa ccttggattc
     3541 accatccagc cgcgacgatg agtagtggga agtttcaaga ctaggttccg gtttcgggtg
     3601 tcaacggtcg cgggcgtgct gctgcttcac gagctcgccg cctgccgcga cctcgccgcg
     3661 gatctagccg cgaacaggtg atggccgggc gctcgcggct tcgagagaca ccggaagaaa
     3721 tgaagccctc ggtcgagggc ttcgcggcgg cctagaaact catgtgttcc aggcattcca
     3781 aaagcggatg aagggactcg aaccctcgac cttctgcatg gcaactgatc gtcggcctgc
     3841 ggatttcccg ctctaaagcg gaaagtggag ggtccgggtc tctcgtttcg gtccaggtag
     3901 tccacgaatc tcagggtgtt ggaccgaaac tggaccgaaa gccctagcta ctacagacga
     3961 tgtagtagtt cgagcggaag gaggcccgcg tacccttgag gcgtgtcccc aagcgccccc
     4021 cgcgaggacc ttccacagtt ccttccacca atgctcgcga gcaccgggcc cgcgcctgat
     4081 ggcgaggggt gggtgcacga ggtcaagtgg gacgggatgc gcgcccaggt ccgcatcgac
     4141 ggccaagtga ccgtgcgctc acggccgggg cgggactgca gtcgcgagtt ccccgagctg
     4201 gccgagctcg ccggccaccc gaccatcgtg cccggctcgc gctcaccatc gcgggactcc
     4261 agtcgcacgt tttcctcgaa gtcgaagtcc agcccggcga tcgccttcac ggtcgcggcg
     4321 aagcggaggg cgtccatcgg cagcttgatc accacgcggc cctcggagcc gtccttcggg
     4381 acgagccgca cccgcgtcgc gtcggtgtcc cgctcgcggg ggcgcgggtt cggcccctgc
     4441 ccgatcacaa aagtgcctcc cgatcgaccg ccgcacccgc gagcgatcgc cgcggtcatg
     4501 cggtctgcgt agctggcacg ttcaggcgtc ggcccctttc ggcccccacc agatgggcgt
     4561 actctgggca aatctgccaa acccagcgat cgaaggggta gccatgccgc tccgagttgg
     4621 ccagcccgct cccgatcaca gcgtgcaggc gtacgtccgc ggagagcgcg cgccagccga
     4681 gttcgcactt tctagtcacc gggggcgctg gcttgtcctg ttcttctatc cgcgcgactt
     4741 cactttcatc tgccccacag agctcgcgtc cttcgccgaa cgccacgacg acttcctggg
     4801 cgagcgtgcg gtcgtccttg cggcgagcac cgacagctac ttcagccaca aggcgtggtt
     4861 cgagtccgat ccgcggctcg ccggcatccg ctatccggtg atcgccgaca gtgcccacga
     4921 gctcagccgc tcgttcgacg tgctcctcga ggacggggcg acgcaccgcg gcaccttcat
     4981 catcgacccg agcggcgtct tagtgcacat gagcgtcaac gagcacgacg tcggccgcaa
     5041 cgtcgacgag gtgctgcgca ccctgcgcgc cttccgcacc ggggcgctct gccccgccaa
     5101 ctggggtccc ggaaaggcga cgctcacgag cgaggacgat tggctcgaga aggtgttccc
     5161 gagcctgacg ggaccggcgc tggactcgct cgccgagcgg gccgaaaagg tgtcggtcgt
     5221 tgcgggggac acgatcgtcg ccgagggcga ccccgccgac cgcttctacg tcatcgcgaa
     5281 gggcgaggtc gcggtcaccc gccgcggtcc cgaaggtcag gagatcgagc tggcgacgct
     5341 cgggccgggg cagttctttg gcgaggtcgg tctcctcgcc gagacgcggc ggacggcgac
     5401 tgtgcgcgcg gtcgccgacg tcgagctgct cgcgttgagc tggcaggaat tccaggcggc
     5461 gctcgagcag tcggatcggg cgaaacggga tttctccgag atcgtccacg agcggctggc
     5521 gggcgcgtcg gcgtcatgag tgcgacgtcg gtcctctacg tggacgacga gacggtggac
     5581 cgcgttatcg gcgctcggca cggcgcactc gttctcgcca aagacgactg cgacaactgc
     5641 gccgcctacg agacccagat ccggcgcctg catgaggagg gtcagttcgg cgatctggtc
     5701 atcggcaaga tcgtgctcac gcagccgggc tgccgggact tcaagcgtgc gaatccgtgg
     5761 ctcggcgggg tcgacttcct tccctacacg ctcctgtacg cgagcggtga gaaagtcgac
     5821 gagttcgcgg gctcgacggg cacctacctc ctcgagcgcg cggcggacgt cgggttcatc
     5881 tagcgcggct cggacctcgg tcggcgaccc tcgctcaccg ctccacccac gcgcagcgac
     5941 tctcgaaggg cctcagcaac ggccagatca accgcgtgat gaaggcggtg gaggccgtcc
     6001 ttgaagacgc ccgcgaccgc gacttgatcg cgcgcaacgc ggcggccaac aagaagctcc
     6061 agttgacggc cgagactcca gaccgcagct acctgcatcc cgtccagatc gaggcgcggt
     6121 tcgaggcggc gcgccagatc gagcaggaga cccgtggcct ctcgcccgcg gacgtggcgg
     6181 cgatccgggc gtcgtcggcc tcggcggtct cgctcgcgcg cgacttcggc gtgagcgacg
     6241 tgctgatcgg caagctgcga cgcggcgagg tcaccgaggt cgggcgctac ggctacgtgc
     6301 cgcggctggc gctcgtcgcg actctcgtct tgggcgggca gcgcatccgc gagctcggcc
     6361 tgctcgacca ccacgacatc aacctcgccg atggctacat ccgtcgtccg cgactcaaga
     6421 cggacgccag cgagcgctcg atcccgatgc tgcccgcgct gcgggacgtg ctgctcgagt
     6481 accgcgcgac ccagccgccg catgtgccgc cgtttgcgac ccgtaccggc aagcgcttca
     6541 ccggcgacaa cgtgcgggcg cggctgcgcc cggtgcgcgc acgcgcgaac gcgctgctcg
     6601 tcgcgcgcgg gcacgagccg atcgcgcgcc tgacgccgca caccttccgg cgcacgttcg
     6661 cgtcgatcct cgcggctgcg tatccccgag actaggtgcg agcagctcat gggccacgag
     6721 gacgccacgt tcaccgcgcg cgtctaccgg caggtgatga agatgcacag cggcggactt
     6781 gaggggctcg aaagggtcat cggctgctcc cgccgggagg ggcatttcct gctcggcggc
     6841 tacgaccagg accccttctg gaccgaaact ggaccgaaag acgcaaaaaa gccctccgcg
     6901 gagggcctgg ccgtttcttg aaaagtcgtg atttgcggcg tttttcagaa gcggatgaag
     6961 ggactcgaac cctcgacctt ctgcatggca agcagacgct ctagccaact gagctacatc
     7021 cgcgtgaggc ggtgagtata gccgcggctc cggctgcggc aggcccttcg taaagcctgc
     7081 ccgaacagca atatccgcaa gcccgcggcg ctccagttcg ttcttccaga gccaggcgat
     7141 tcgcccggcg ggccctgccc cgccgctccg ggcgagaggc ggtcccaaca ccggagcacc
     7201 aatgttcaga ggaaagcagc acacgcatca ggagccggcc gcgcgcccga tcgctcgtcc
     7261 ggtcgccgaa acgagccctg acgccggggg agctctagcc agcctccgcg cagaggcgag
     7321 ggagcgtgag cgcgacgccg cgctcgaggc tcgcctggat gctctgggtc gcgccgagca
     7381 ggcggcccgt gagcggtatg agaccgaagt gcgacgctgg acgcgcgaga gcgccgacgc
     7441 tcgcggccgg ctgctcgagg ctggcgaggc gttggccgcc gcgaggaacg aagcccagcg
     7501 gtaccaggtc gacgcggcgc gcctggggga ggagcgagag cagatcgcgg gccagctcgc
     7561 cgacgctcgt gaggcgctcg cgaccgcggc cactgaagcc gagcggcagg cggaggagca
     7621 cggcgctctc gagcgccgcg tggaggagct gcagcgggag agggacgagg ctcggggcga
     7681 cgccgagcga atcgcggccg agctgaccgc tgctcgtcgc gaggccgagg aggcggcgag
     7741 cgcggctcac gaacagttgc aggcgcggac ggagctcgag cagcgcatgg agcgtgtgca
     7801 ggccgaggtc gaggaccgtg ccgccgtgct cgccgaggcc gagaggcagg tcgacacggc
     7861 gcttcgccgg cggatggact cgttcgtcga ggcggagcgc gaggctcggg cggctcaaga
     7921 gcgcgacacc gccgaagcgg ccgcgcgtac ggccgagctg gacgcgctcg cggcccggat
     7981 cgtgcgggcg cgcgaggtgc tcgccgagct ccgcgaggag agtcgccgcg aacaggaccg
     8041 ccacgcgagg ctcgacgatc gtctgcgcga catcatcggc cctgaaccgg agccggccga
     8101 gcaggaggca cagcgggtca cggagcgtga ggaacctccc gctgagcacg agccctgggt
     8161 tggtaacggc gaatcgactg atgccgccga ggcgcagcgg gcgggccgcc gcaagctctt
     8221 cggccgccgc aagcagggcc cgttcatcgg ccgcccgggc tactgcagcg tgtgctcacg
     8281 ggagctgatc gtggagagca aggaggagct cgccgcgagc gggtggctga tcagcgacgg
     8341 agaggccgtc tgtctgtcgt g
//
LOCUS       DASVXS010000304         6312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_705515, whole genome shotgun sequence.
ACCESSION   DASVXS010000304 DASVXS010000000
VERSION     DASVXS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705515"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VF752_17050"
     CDS             complement(<1..574)
                     /locus_tag="VF752_17050"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX8857304.1"
                     /translation="MAPVGALGDSFRSFFDAVDKFFANLAHVQWGALLIALCAFGIYL
                     TLRARASYNILRAAYPNERFPFRLIWGAYFAGYGFNSVIPARGGDVVRLFLTKTSIPG
                     SSYSAVAASFVVELIYDLCMGIVILTFAFSQGVFPKPPDFSKLPAFDLAFFAQHPQFT
                     LFVLTALAVAVMIGIALTSERIRAFWSHVRQ"
     gene            complement(578..970)
                     /locus_tag="VF752_17055"
     CDS             complement(578..970)
                     /locus_tag="VF752_17055"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX8857305.1"
                     /translation="MTWLWLALAAGVVAWLLFVLVLYAAGRREDARALAGFIPDCVVL
                     FKRLSTDSGVPRRRRVLLLGLIAYLAMPFDLVPDFIPVAGQLDDAILVALVLRGMMRG
                     AGPSVVREHWPGPERSLNAVMRLAGGYR"
     gene            complement(972..1604)
                     /locus_tag="VF752_17060"
     CDS             complement(972..1604)
                     /locus_tag="VF752_17060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8857306.1"
                     /translation="MEHGHAHADAHSHGLLDPSIRRSRDGIRAVAVSLGVLAATAAVQ
                     AAILVATGSIALLADLIHNVGDALTAVPLGIAFALRSERAERAAGLAVVLAIFVSACV
                     AGVEAVRRLVEPSAPDHLLALALAGVAGFAGNWLAAMVRTRAGRRLDSPALVADGNHA
                     RADAYVSLAVVASAAVVALGFDLGDPLIGLAITLVILRITWHSWQTVRRD"
     gene            complement(1678..1998)
                     /locus_tag="VF752_17065"
     CDS             complement(1678..1998)
                     /locus_tag="VF752_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857307.1"
                     /translation="MYASIRSYRINDSQVDDLMHRIDTEFADSVQDQPGFISYQAVYT
                     GGGTLCTVTVCRDEATCDRTNEMAAMFVRESLSDMEIERTDVKGGEVMVSRAASEVLQ
                     LVHH"
     gene            complement(2128..2601)
                     /gene="bcp"
                     /locus_tag="VF752_17070"
     CDS             complement(2128..2601)
                     /gene="bcp"
                     /locus_tag="VF752_17070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX8857308.1"
                     /translation="MLEAGEKAPDFELQNQDGEPVRLSDLRGRTVVLYFYPKADTPGC
                     TTQACGIRDRSGEYVDAGATVIGVSPDDVGAVKKFADKYSLDFTLLADPDHAVAEAYG
                     VWGEKKNYGRTYMGINRSTFIIGADGTIERVFPKVSPKTHDDVVLAALGELREVA"
     gene            complement(2623..3471)
                     /locus_tag="VF752_17075"
     CDS             complement(2623..3471)
                     /locus_tag="VF752_17075"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX8857309.1"
                     /translation="MPELPEVEITARRLDRALAGARVESALAPGMVTMKTFDPPLDSL
                     AGREVTAVRRRGKMPIVEFGDVGLLIHLMSAGRLQLFDKRASLRDRRSRILIRLEDGR
                     ELRLREFGTQQRAWAKLLPVEEVDADEAIATLGPEAFPVPPLDELAELVDQPRHLHPM
                     LRDQRTIAGIGRSWVDEILWTAKLSPFRQGSDLAEEEVEELREAIEECLGSTLDHYEE
                     VVGDSLPDKAPMPLKVHGKQGDPCPRCGATLAAVFFKEHVTTYCPQEQTAGRVLKDRR
                     LSRLLK"
     gene            complement(3497..4900)
                     /locus_tag="VF752_17080"
     CDS             complement(3497..4900)
                     /locus_tag="VF752_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8857310.1"
                     /translation="MRRFAALLAAAGLALLLAAPANAEVKTETFKYGPVEVNGFEVKL
                     NNDFATPHPDVDGFITAMNVDVVDANGKPVPIQRIMLHHIVFGNLGQKFGDKRDATCN
                     AFTNFDSRTQLPALTERFYGAGEERARLQLPPGYGYQIKKGERWSLYYMLMNHKSKKD
                     KVYIQYTVTYDTNPDLKPVTPYWLDVRNCWVDPVYNVPGGGKPGSTDRKATNWTVPYS
                     GRIVAAGGHVHGGGKDLELREPMCGNRLLYKSTPTWGLPSHPFYNVRPILHEPGPINM
                     SGFNSAQGIPVAGGEKLKFISNYDEQYVHTRVMGISLLYLAHDDSVTKPCGAMPTDIR
                     TFGSPIPGRKETPRFTVPITAVGRDGVARSVSKAPGGTQTLRGDARVNVKDFYFSRPN
                     VIVPQGSKLTWKFFGSTLHNVTLANGPRGFASDNASQNEQYSYKFKVPGTYRLFCALH
                     PVDMTETVTVLPKGKKK"
     gene            5023..5643
                     /locus_tag="VF752_17085"
     CDS             5023..5643
                     /locus_tag="VF752_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8857311.1"
                     /translation="MEVRTAKGAREVQAHVDGRTARSDRTRDAIVAAMLDLYEEGDVK
                     PNAQRIAARAGVSERTIFQHFHDREALFAAVADRQWERVSALADVISPDLPLPERIDA
                     FLRARVRQLEVMTPVRRAAILTEPFSGTVAAQLRASRDHGRRQVEDVFANELSGCAGP
                     EDRAQLLAGLASATSWNTWESLRDHQGLSKIEAREVMRRMIAALLS"
     gene            5654..6088
                     /locus_tag="VF752_17090"
     CDS             5654..6088
                     /locus_tag="VF752_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX8857312.1"
                     /translation="MKIAVAADERTGVADAVLDELRKRGHEPLPHGALAEAERDDWAW
                     ASEAAARDVAEGRAEQGIVCCWTGTGASIAANKVEGIRAALCGDAETARGARRWNDAN
                     VLALSLRSTSQAELEEILDAWFEGEPSRDEDDVANVRHVDEI"
     gene            complement(6085..>6312)
                     /locus_tag="VF752_17095"
     CDS             complement(6085..>6312)
                     /locus_tag="VF752_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857313.1"
                     /translation="SRRIRFALSEPARLTGSVKKGARRVQKISITGRKGTNSVKLSRK
                     LKAGRYRLTLKAVDAAGNTSKTFTKTFRLGR"
BASE COUNT         1012 a         2100 c         2212 g          988 t
ORIGIN      
        1 cctgccgcac gtgcgaccag aacgcgcgaa tccgctccga ggtgagcgcg atgccgatca
       61 tcaccgccac agcgagcgca gtgagcacga acagcgtgaa ctgagggtgc tgcgcgaaga
      121 aggcgaggtc gaaggccggg agcttcgaga agtccggcgg cttcgggaac acgccctgcg
      181 agaaggcgaa cgtgaggatc acgatcccca tgcacaggtc gtagatcagc tcgacgacga
      241 agctggccgc cacggccgag tagctggatc ccggtatcga tgtcttcgtg aggaagagac
      301 gcacgacgtc gccgccgcgc gccgggatca cgctgttgaa cccgtagccc gcgaagtagg
      361 cgccccagat caggcggaac gggaagcgct cattcgggta ggcggcgcgc aggatgttgt
      421 aggaggcgcg cgcccgcagc gtgaggtaga tcccgaacgc gcagagggcg atcagcagcg
      481 caccccactg cacgtgggcg aggttcgcga agaacttgtc gacggcgtcg aagaacgagc
      541 ggaacgagtc gccgagcgcg ccgaccgggg ccatggctca acggtatccg cccgccagac
      601 gcatgacggc gttcagcgag cgctccggcc cgggccagtg ctccctcacc acggaagggc
      661 ccgcgccgcg catcatgcca cgcagtacca gtgccaccag gatcgcgtcg tcgagctgtc
      721 cggcgaccgg gatgaagtcg ggcacgaggt cgaacggcat ggcgaggtag gcgatcagcc
      781 cgagtagcag gactcgccgg cggcgcggaa cgcccgagtc cgtcgagagg cgcttgaaga
      841 gcaccacgca atcgggaatg aagccggcaa gcgctcgcgc gtcttcccgg cgcccggccg
      901 cgtacagcac cagcacgaac aagagccacg cgaccacacc ggcggcgagc gctaaccaga
      961 gccacgtcat ctcagtcgcg gcgcaccgtc tgccaggaat gccaggtgat gcgcaggatc
     1021 acgagtgtga tcgcgaggcc gatcagcggg tcgccgaggt cgaagccgag ggccacgacc
     1081 gcggcgctcg cgaccacggc caggctcacg taggcgtcgg ctcgggcgtg gttcccgtcg
     1141 gctaccagcg ccgggctgtc gagacggcgc ccggcgcggg tgcggaccat tgcggccaac
     1201 cagttgccgg cgaagcccgc cacgcccgcg agggcgagag cgagcaggtg gtccggcgcg
     1261 ctcggctcga ccaggcgcct gaccgcctcg acgcctgcca cgcaggcgct gacgaagatc
     1321 gcgagcacga ccgccagccc ggccgcgcgc tcggcccgct cgctccgcag cgcgaacgcg
     1381 atgccgagcg gcaccgccgt cagcgcgtcg cccacgttgt ggatcagatc ggccagcagg
     1441 gcgatgctgc ccgtggccac gagaatggcc gcctggaccg ccgcggtcgc cgcaagcact
     1501 ccgagcgaga cggccaccgc gcggatgccg tcacgcgagc gcctgatcga agggtcgagc
     1561 aacccgtgcg aatgtgcgtc tgcgtgcgcg tggccgtgtt ccaccgcccg caggttagaa
     1621 gaggcggcgt ccggccccgg aggcaggccc agggccggaa atgccgcaat cggggcttca
     1681 gtggtggacg agctggagaa cttcgctggc tgcccggctc accatgacct cgccgccctt
     1741 cacgtcggtg cgctcgatct ccatgtcgct caggctttcg cggacgaaca tcgcggccat
     1801 ctcgttggtg cggtcgcacg tggcctcgtc acggcatacg gtcacggtgc agagggttcc
     1861 gccaccggtg tagaccgcct gataggagat gaatccaggc tggtcctgaa cgctgtcggc
     1921 gaactccgtg tcgatgcgat gcatgaggtc atcgacctgg gagtcgttga tgcggtaact
     1981 gcggatgctt gcgtacaaga gactcctcct tcgaagacgc aggcgtttcc gaggttcggg
     2041 cgctgcccga cgcggcttag tccacagctc tcctctcagc cgggcgtggc gcgaacctac
     2101 cggctcgcgc ggggcggcgg caagtaccta tgcaacctcg cgcagctctc cgagggcggc
     2161 aagcacaacg tcgtcgtgcg tcttcggcga caccttcggg aagacgcgct cgatagtgcc
     2221 gtcggcgccg atgatgaagg tcgagcggtt gatccccatg tacgtgcgcc cgtagttctt
     2281 cttctcgccc cagacgccat aggcctcggc caccgcgtgg tccggatccg cgagcagcgt
     2341 gaagtcgagc gagtacttgt cggcgaactt cttcacggcg ccgacgtcgt cgggcgacac
     2401 gccgatcacg gtcgcgcccg cgtcgacgta ctcgccggag cggtcgcgga tgccgcacgc
     2461 ttgcgtggtg cagccgggcg tatcagcctt gggatagaag tagagcacca cggtccggcc
     2521 gcgcaggtcg gagagcctca cgggctcgcc gtcctggttc tgcagctcga aatcgggtgc
     2581 cttctctcca gcctcaagca tgtcgggctc ctctcggggg ttctacttga gcaggcgcga
     2641 gagccggcgg tccttcagga cccggccggc cgtctgttcc tgcgggcaat aagtcgtcac
     2701 atgctccttg aagaacacgg cggcgagcgt ggcgccacag cgcgggcacg ggtctccctg
     2761 cttaccgtgg accttgagcg gcatcggcgc cttgtcggga agcgagtcgc cgaccacctc
     2821 ctcgtagtgg tcgagcgtgg agccgaggca ctcctcgatc gcctcgcgca gctcctcgac
     2881 ctcctcctca gcgagatccg agccctgccg gaacggcgag agcttcgccg tccagaggat
     2941 ctcgtcgacc caggagcggc cgatccccgc gatcgtgcgc tggtcgcgca gcatcgggtg
     3001 gaggtggcgc ggctggtcga ccagctccgc gagctcgtcg agcggcggca cggggaacgc
     3061 ctcgggcccg agtgtcgcga tcgcctcgtc ggcatcgact tcttccacag gcagcagctt
     3121 ggcccacgcc cgctgctgag tgccgaactc gcgcagtcgc agctcgcgcc cgtcctcgag
     3181 ccggatcagg atgcgcgagc ggcggtcgcg cagggacgcg cgcttgtcga acagctgcag
     3241 ccggcccgcc gacatcaggt ggatcagcag cccaacgtcg ccgaactcca cgatcggcat
     3301 cttgccgcgg cggcgtaccg ccgtgacctc gcgacccgcc agcgaatcca gcggcgggtc
     3361 gaacgtcttc atcgtcacca tgccgggcgc gagcgcggac tccacgcgcg cgccggcgag
     3421 cgcgcggtca agccggcgcg ctgtgatctc gacctcgggt agctcgggca ctacctcaaa
     3481 tgtagggcga ggctccctac ttcttcttgc ccttcggcag caccgtcacc gtctccgtca
     3541 tgtcgacggg atggagcgcg cagaagagcc ggtacgtgcc gggcaccttg aacttgtagc
     3601 tgtactgctc gttctgggac gcgttgtcgg aggcgaagcc gcgcggtccg ttcgcgagcg
     3661 tgacgttgtg aagcgtgctg ccgaagaact tccaggtgag cttcgaccct tgcggcacga
     3721 tcacgttcgg cctcgagaag tagaagtcct tcacgttcac gcgggcatcg ccacgcagcg
     3781 tctgggtacc gccgggagcc ttcgagaccg agcgcgcgac gccgtcgcgt cccacggcgg
     3841 tgatcggcac ggtgaagcgc ggggtctcct tgcgccccgg tatcggcgag ccgaacgtcc
     3901 tgatgtcggt gggcatcgcg ccgcacggtt tcgtgacgct gtcgtcgtgc gccaagtaca
     3961 gcagcgagat gcccatcacc cgcgtgtgga cgtactgctc gtcgtagttg gagatgaact
     4021 tgagtttctc tccaccggcg accgggatgc cctgggccga gttgaagccg ctcatgttga
     4081 tcggccccgg ctcgtgcagg atcgggcgca cgttgtagaa gggatgcgag ggcaggcccc
     4141 aggtgggcgt ggacttgtag agcaggcggt tgccgcacat cggctcacgc agctcgaggt
     4201 ccttgccccc gccgtgcacg tggccgccgg cagcgacgat gcggccgctg taggggaccg
     4261 tccagttcgt ggccttcctg tctgttgagc ccggcttgcc accgcccggc acgttgtaga
     4321 ccgggtcgac ccagcagttg cgcacgtcca gccagtaggg cgtgaccggc ttgaggtccg
     4381 ggttcgtgtc gtacgtgacc gtgtactgga tgtagacctt gtccttcttg gacttgtggt
     4441 tcatcagcat gtagtagagg gaccacctct cgcccttctt gatctggtag ccgtagccgg
     4501 gcggcagctg caggcgcgcc ctctcctcac cggcgccgta gaagcgctcg gtgagcgcgg
     4561 gcagctgcgt cctcgagtcg aagttcgtga acgcgttgca cgtcgcgtcg cgcttgtcgc
     4621 cgaacttctg ccccaggttc ccgaagacga tgtggtggag catgatgcgc tggatcggca
     4681 cgggcttgcc gttcgcgtcg accacgtcga cgttcatcgc cgtgatgaag ccgtcgacgt
     4741 ccggatgcgg cgtggcgaag tcgttgttga gcttgacctc gaacccgttg acctcgacgg
     4801 ggccgtactt gaacgtctcg gtcttgacct cggcgttggc gggcgctgcg agcaggaggg
     4861 ccagtcccgc tgcagcaagc agagcggcga atcggcgcat gcgtttttcg agtcctcggt
     4921 gaacgtcgcg gggctacgga agtcgcgacg cctccctcac ctcatcgtcg gccaccctac
     4981 cagtgcttgc accctcggtg aaaaaaggtg tagcttcggg gcgtggaggt tcgcaccgcg
     5041 aagggggcac gcgaggtgca agcgcatgtc gatggacgga cggctcggtc cgatcgcacg
     5101 cgcgacgcca tagtcgcggc gatgctcgac ctctacgagg agggagacgt gaagccgaac
     5161 gcgcagcgca tcgctgcgcg cgccggtgtc tccgagcgca cgatcttcca gcacttccac
     5221 gaccgtgagg cgctgttcgc cgccgtcgcg gaccggcagt gggagcgcgt gagcgcgctt
     5281 gcggacgtga tctcgccgga cctgccgctg cccgaacgca tcgacgcgtt cctgcgtgcc
     5341 cgggtgcgcc agctcgaggt catgacgccc gtgcgccgcg ccgcaatcct caccgagccg
     5401 ttctccggaa ccgtcgccgc gcagctgcgc gccagtcgcg accacggacg tcggcaggtg
     5461 gaggacgtgt tcgcgaacga gctgagcggg tgtgcgggtc ccgaggaccg cgctcagctc
     5521 ctcgccgggc tcgccagcgc gacgtcgtgg aacacctggg agagcctgcg cgaccaccag
     5581 gggctgtcga agatcgaggc gcgcgaggtg atgcgccgca tgatcgccgc gctgctgtcc
     5641 tagcctttcg actgtgaaga tcgccgtcgc cgccgacgag cgcaccggcg tagccgacgc
     5701 cgtgctggac gagctgcgca agcgcgggca cgagccgctg ccgcacgggg cgctcgccga
     5761 ggccgagcgc gacgactggg cttgggcgag cgaggcggcc gcgcgcgatg tcgcggaggg
     5821 ccgggcggag caagggatcg tgtgctgttg gacggggacg ggcgcctcga tcgcggcgaa
     5881 caaggtggag ggcatccgcg ccgcgctctg cggcgacgcc gagactgcgc gcggcgcgcg
     5941 caggtggaac gacgcgaacg tgttggcgct gtcactgcgg agcaccagtc aggccgagct
     6001 cgaggagatc ctcgacgcat ggttcgaggg cgagccgagc cgcgacgagg acgacgtggc
     6061 gaacgtccgc cacgtggacg agatctagcg gcccaggcgg aaggtcttcg tgaacgtctt
     6121 cgacgtgttg ccagccgcgt cgacggcttt cagggtgagc cggtagcggc ccgccttcag
     6181 tttgcgcgac agcttcacgg agttcgtgcc cttccggcca gtgatcgaga tcttctgaac
     6241 ccgccgcgca cccttcttca ccgatccggt cagcctggcc ggctccgaca gcgcgaaccg
     6301 gatccgccgc ga
//
LOCUS       DASVXS010000305        25450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1272143, whole genome shotgun sequence.
ACCESSION   DASVXS010000305 DASVXS010000000
VERSION     DASVXS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 25450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25450
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272143"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..701)
                     /locus_tag="VF752_17100"
     CDS             complement(30..701)
                     /locus_tag="VF752_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857314.1"
                     /translation="MDCRSASVEREARPLCVPPKPGAGALYYVFDPASPANRLFHGFS
                     ADPSRLAHSNRVLRGFATDQIRAQPLDYARAAGSDFLRFLTPGERSRGTSDRALELAT
                     VLNPVVRDRWFPRYEPPHATSAARDYVRLVHAPRWLLALLSLASLAVVALPGAVHGRR
                     ETFLFTGAGLAMLVGAAATSEFVLRYLEPSVPLLATGGALACSALAARVRAPRPEPTA
                     VASPT"
     gene            complement(670..1395)
                     /locus_tag="VF752_17105"
     CDS             complement(670..1395)
                     /locus_tag="VF752_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857315.1"
                     /translation="MAGARIGAHKWFAGVLALGLAARLLVWLGAWPGLLYTDSWAYAD
                     AAYKGQYLSDPLHPAGYPLFLHVLALPGRSLAALTLVQHLMGVATGVLLYALLVALGL
                     RRGLAAAAAALVLLDGWAIALEQHLLAEALFTLVLVAALALAAWPGERGIARLAGSGG
                     LLAAAVLIRPVALFAVPVWIAYLVWTRPAWRAIAVAAAACALPLVLYPRQAGQLRLHV
                     GVGLAALRACGRVRGLPQRERRA"
     gene            complement(1444..2430)
                     /locus_tag="VF752_17110"
     CDS             complement(1444..2430)
                     /locus_tag="VF752_17110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX8857316.1"
                     /translation="MSSARLTVIGEALIDLVPTGPGQYRAHPGGSPFNVAVALARLGN
                     RTALMARLSDHGFGRLLRETATGEGVDLSASARAAELTTLAAVSMDMPGQASYEFYSE
                     GTADWQWSTAELRKRPTDTAVLHFGSIASWTPPGSKRIDQLVAEARARNGVLVSYDPN
                     IRPHLLDAPTRGRRLVERSVRRSHVVKASREDIEWLYPSRSVDDIATRWNDLGAELVV
                     VTEGPNGATAFREGTEALHRPGRAVEVVDTIGAGDAFTAGLLSGLVRRALHAPQQVAA
                     IPDGALAEVVDEAVLVSSLTCGRAGADPPRLASAEIGARSLTVEDFATAAPA"
     gene            complement(2427..3215)
                     /locus_tag="VF752_17115"
     CDS             complement(2427..3215)
                     /locus_tag="VF752_17115"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8857317.1"
                     /translation="MSRHALVTGAAGDLGRAACVALAEDGWSLFVSDHPGQAARLDAT
                     RDACVASGAEVVASTFDVTDHAVVGREVGMLAERHGVPTAVLAGAGLQGDFAPVQDYD
                     VDVARRVIDVNVMGVFNTLKATAELMIGAGTGGSIVAIASMAGVSGVPNMPAYSASKA
                     AVIGLAKSASRDLAPHGIRVNAVSPAFIGPGAMWENQVAQQAAVGSQYYSTDPDEVAR
                     QMIGLVPLRRYGSPAEVADVICFLAGDRSSYLTGINVEIAGGAV"
     gene            complement(3225..4319)
                     /locus_tag="VF752_17120"
     CDS             complement(3225..4319)
                     /locus_tag="VF752_17120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8857318.1"
                     /translation="MTAVRYEHATRTYEGTDVPAVDDLELDVGDGELLVLVGPSGSGK
                     STALRMLAGLEPLDAGAVYIGDRDVSDVRPRDRDIAMVFQNYALYPQLTVAENMGFAL
                     KQHGVPKEERTARVRETARILDLVPFLDRKPKQLSGGQRQRVAMGRAIVRQPAVYLMD
                     EPLSNLDAKLRVQTRTEVVELQNRLGVTTVYVTHDQVEAMTMGDRVAVLRDGVLQQCD
                     APGTLYHEPNNLFVAGFIGSPAMNLVNADGNRPLRIGGAQIELDPSANGAVADADGEI
                     TVGLRPEALRVGDGPLRAHIRTVEDLGSEVFVHVAIDHQGESIPLVAKMSPPFHGEPG
                     DNVGLELVGTTHLFAGDGARVTSTTHASLR"
     gene            complement(4330..5187)
                     /locus_tag="VF752_17125"
     CDS             complement(4330..5187)
                     /locus_tag="VF752_17125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8857319.1"
                     /translation="MSAVPAPRVVGESRWVNRSVGLLAWIVGLVFFFPVFWMVLNSFK
                     TEQDANTSPKFIFHPTLDRYRDVTRSAGGVLNFGEAFTNSAVVVLVSVAIVMLLAIPA
                     AYALAVKPVHKWRDVLFFFISTKFLPIVASILPIWILARNFHLLNTRMSLIILYTAIN
                     LPLAVWMLRSFFAEIPRELIEAAEIDGAGLKGQLTSIILPIAAPGIAATALLCVIFAW
                     NEFFYAVQINPVQGSTVPIWVTTNISTRGDFLAKLSAASVLACIPVVLAGWIAQKRMI
                     RGLAMGAIK"
     gene            complement(5184..6122)
                     /locus_tag="VF752_17130"
     CDS             complement(5184..6122)
                     /locus_tag="VF752_17130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8857320.1"
                     /translation="MSTAAGATTASQPRQRRLTPPGLSESWRRRLPLLPALIFTIVVT
                     QVPFVMSIWYSLTDWHVVPPGPRKFIGLDNYGHLFSDHFFRDAVWVSVRLAVIPVFGA
                     LVLGTAFALLLDRKFFGRGVVRTLLITPFLLMPVVVGLIWKNQMFNDLYGVINWGIDK
                     LGGTPVDFVARHPTTAIAVTLIWQWTPFMMLIMLAGLQSQPADVLEAAKVDGATPWGI
                     FRQLTLSHLRPYMELGILLGTIYLIQVYDLVAVMTGGGPGSTNVPYFVFQKSIGGGWE
                     FGQASSYSIVVVLASIVIATISLRVLSNLLKGEEIA"
     gene            complement(6180..7556)
                     /locus_tag="VF752_17135"
     CDS             complement(6180..7556)
                     /locus_tag="VF752_17135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX8857321.1"
                     /translation="MRGMTGARSAAAVLAVAGVAGVVATGCGGGGGGGGSSNNSSSKS
                     INVAIVDNPQMKDIAKLTPSLFTKKTGIKVNYTVLDEGTLRQVTTRDVAAGGRQFDVA
                     MIGPYEAPQFGQAGNLVDLTPKAQNSSAYQFNDIIPAVRNALSADGKLYAAPFYGESS
                     FLMYRKDVMKKAGITVPPRPTWDQVAAIARKVNSSKMAGICLRGKPGWGDLGASFTTV
                     LNTFGGTWWSAKPDGSVDKAMVNQPQFKQALQFYTGLIHDAGEKDAANSSFNECLTQY
                     KDSKVAMWYDATVAASNLEASDSPVKGKNGYAAAPVKETKDAGWLWSWALSIPKTSSK
                     QDVAWKYIEFATGPQYLKEAGAKIPGGWAALPPGARKSLYDIPQYKKAAAAFAKPTLD
                     SISSAPITNPGTTKRPGLPGVQYVGIPQFQDVGNQCTQQFSAVIAGRSSIDSALSNCQ
                     QVASQVAQ"
     gene            7778..9157
                     /locus_tag="VF752_17140"
     CDS             7778..9157
                     /locus_tag="VF752_17140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX8857322.1"
                     /translation="MPVPVYDRARLAARIVHIGVGGFHRAHFALYTHELAEAGSDWGI
                     VGLGLLPRDAEMAAALTGQDFLYTLIEKGNGEPCAEVIGSITGYVHAPDGRDGAVDEL
                     IASPTTAILSLTVTEAGYSDEAEARHSFDRIATALATRRARSAGPLTILSFDNIPGNG
                     DAARHATLSAATRRDGELAAWVQENCTFPNSMVDRITPVTADSDRTWLLENAGIEDRW
                     PVVAEPFRQWVVEDEFAAGRPSWDQAGAIFTDRVHDWERYKLRFLNAGHSSMAYLSAL
                     AGITFVHEAMAIRAIQTFLEALLYREAMPTVAAIPGHPREDYVASVLERFGNSGVRDQ
                     IARLCIDGSAKFPTFLIPTIVRQLEAEGPIDRAATALAGWARYLGVIDPEHQAFDASG
                     DTARRYAAAATGDPLAFLDYKAVFPPELRGSTRFRTAFAESYRRITEHGPISAMEPET
                     SGQRTGDAR"
     gene            9154..9942
                     /locus_tag="VF752_17145"
     CDS             9154..9942
                     /locus_tag="VF752_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX8857323.1"
                     /translation="MTISPPEGPRLPHDRQHAIYMLALRQGSVDVADLARRYGVTTET
                     IRRDLSDLQKRRLLRRVHGGAVPVERMNHEPMVDARDMVNAEEKLRIATMAVQEVPER
                     GSVIIDSGSTGQRLADVFPVERDIHVVTNSLITALTLSRRGLRDLTVLGGGVRTKRLA
                     MVDDTTRRELEHMAIDVLFISCDGLSFKHGLTTPYREEHTIKRAMIERASRVVALVDS
                     SKFGNVQMFGFASFDEIDVLVTDTEADADAVDILSTHGIIVHRA"
     gene            9935..10729
                     /locus_tag="VF752_17150"
     CDS             9935..10729
                     /locus_tag="VF752_17150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8857324.1"
                     /translation="MRDSGAAREPAELERIAARAAQAAAGLVTAAYGSPDPVGRKSSP
                     TDVVTQTDLSAEELVRGVLREETPAAGVLGEEGGKTARGARLQWVIDPLDGTINFLYG
                     VPLFAVSIAAALDGEVVAGAVIDVLRDELFSAHRGGGARLNGKAISVSACASLSEALV
                     ATGFSYQAELRSVQGEVAQRLLSRARDLRCFGSAALELCWVACARLDGYFQRDTEIWD
                     RAAGGLIAQEAGARVEMPCPENDDLVIAAAHGVFDDLWAAVHFAAA"
     gene            complement(10711..11529)
                     /locus_tag="VF752_17155"
     CDS             complement(10711..11529)
                     /locus_tag="VF752_17155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX8857325.1"
                     /translation="MTRLLGDLVARGTRSSIHAYGRGVVIKVPHASTPTDWIEFEAEY
                     AEAAREAGAPVPRLLGIEPISGRAASVWERAEGTSMWQHVIDRPQSSRELGRMLADTQ
                     CGLFELVPPVTLPNQRDRLVSKIRWSAATVDVSVRRALELLPSPANAPRLCHGDLHPS
                     NVILCDRGPMIVDWFDASRGDPVADVARTLVTLLGDGTSGPRHLPGSDAATLAALTEG
                     YLERLRTALEIDDELLARWQAIQAAARMAEGVPRAALFQVWTRYESPALQAAAK"
     gene            11622..12017
                     /locus_tag="VF752_17160"
     CDS             11622..12017
                     /locus_tag="VF752_17160"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8857326.1"
                     /translation="MSQSIVDLSLRLYDALNRRDWDAFRELLEDEVEIESRLVVMEGP
                     YHGHAGARRWWNDFLGAFPDYEVEVQELRDLGDVTLAFIRGWGHGVASATPIIDPFWH
                     PVRWVDGKCVWWRNCVTEEEELQAIGALP"
     gene            complement(12014..13576)
                     /locus_tag="VF752_17165"
     CDS             complement(12014..13576)
                     /locus_tag="VF752_17165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8857327.1"
                     /translation="MSSAAASPAPRPVSRVDPGAVLALVAVAQVMVVLDATVVNVALP
                     TIKRDVGFSEQSLSWVLNAYTLVFGGFLLLGGRLADRLGRRRLLVAGIALFAGASLVC
                     GVSQSEGMLLGARAAQGLGGALVSPAALSIILTTFAEGPERNRALAVWGAIAGAGGAV
                     GLLLGGLIVEALTWRWVFFINVPIGAVVLALAPRIIPESRTESGTQGGYDAEGALAIT
                     LGTIALVYTLIKADSWGWTSGRTLAGFAIAAALIVAFVVIEHRQPEPLVPLRIFSNRG
                     LAASDATMLVVAAALFGVFFFCTLYLQQVLGYSALKTGVAYLPLSLTLVGASALASRF
                     VDRFTPKPVLAAGLLISTAGFVLLTGVSGHSDYASHVLPAMLVLGTGLGMSFVPITIA
                     ATNGVAAADSGLASGLLNTTQQVGGSLGIAILSSVSNSRVASSLAAGSSLPVALTHGF
                     KGAFLAAAILCALGVAVALVLLPGRRRKPEDEAVETVALSFARCPGAPYCGHLARIVA
                     LGRRMRSAVSSS"
     gene            complement(13639..14343)
                     /locus_tag="VF752_17170"
     CDS             complement(13639..14343)
                     /locus_tag="VF752_17170"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX8857328.1"
                     /translation="MVLQACLNGTRSRLEHEAVPITPAQLATDARACRSAGARSMHFH
                     PRDENGIETLDPGICAEALRAVRSVVATAELGLSTGIWIEGSATATRAAVESWHELPD
                     MVSVNLSEDGALELIELALRRGIEVEAGLASVADAEKLVESEFAVRCRRALVEVEGDD
                     SASAVALAGEIERVVYPTGIEQLHHGIGGATWAVLEAARDRRHSIRVGLEDTLVMPDG
                     STARSNARLVRAAASL"
     gene            complement(14347..15381)
                     /locus_tag="VF752_17175"
     CDS             complement(14347..15381)
                     /locus_tag="VF752_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8857329.1"
                     /translation="MLAVTFQEPGRVAVEERPEPEPQAPDDAVVAVEASGICGSDLHI
                     FHGRVGVEPGFTIGHEYVGRVVEAGDAVTSVSVGDRVLGTFHTACGTCFFCRRGVFQK
                     CDEARVFGHGKLLGSLQGTQAERVLVPRANMTLRRVPDGLSDDAALFAGDVMGTGYHA
                     VVSAGLEPGDTCAVIGLGPVGLCAVMAARVAGAARVIGIDTVPDRLATARTLGAEPVH
                     ATEEDPRAAAKAATDGRGVDIAIEAVGTGQTLDLALRLARKAGTVSLVGVHAEREEVN
                     VGMAWLKSLTVLTGHANVMGHLDSVLALMAAGRLDPTPLVTHHMKLEQAPQAYETYAR
                     HEALKIVLSA"
     gene            complement(15393..16973)
                     /locus_tag="VF752_17180"
     CDS             complement(15393..16973)
                     /locus_tag="VF752_17180"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8857330.1"
                     /translation="MPPPDSSTPQRLGVVGAGTMGAGIAQLGVLAGIETRLHDPFEDA
                     LERGIERLREGLRRGAERGRWSEDEARAAEERLHAAPSVDDLSETELVIEAAPERPEL
                     KRELFARLSEVCSSETVLATNTSSILVSSIATAAARPENVVGMHFFNPPPLMKLLEVI
                     AAAQTGERALAVARATGEAMGKHVIVAVDGPGFLVNRCGRPFGSEALRLLQERVATVE
                     QIDRICRLGGGFRMGPFELMDLVGIDVGFEVAQSFTAQSFGEPRWKPNMLQAQKVAAG
                     QLGRKTGRGWYEYGDGAHRPDDADPPPLGGGDGRPVAIDGSGRLASALRERARDAGFD
                     AREASEFHAEELWLLVDAESPAPVADEDLLGDSPTGPPLLALCADLSLAQRGQPEACG
                     FHLLPPLGDSKLMELTRLPSTPALTQERAEEFATALGYHHEWVCDAPGLVLGRIVCQL
                     ANEAAFAIGEGVGTPEDVDAGVTLGLNHPRGPVEWSRAIGLDHVLATIDGLYEERREE
                     RYRAAPLLRRSVAASEDL"
     gene            17167..18711
                     /locus_tag="VF752_17185"
     CDS             17167..18711
                     /locus_tag="VF752_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX8857331.1"
                     /translation="MPETKMLFNLNRIGARIALAGLLGAAAIGALASPAHASRTQESV
                     MQDDRLLIKSGSAVREQALNDMQSLGVDTVHSLAVWRDFAPNADSRTKPSGFDGSDPA
                     AYPPTAWDALDDLVRTLHARGMSLILTPTGFTPSWAGSCGSAHNRQVCGPNSAEFGKF
                     VAALGKRYSGTYHDENQGGAVLPAVTRWSIWNEANLGAWLQPQFVVRKGRAVPKAPHI
                     YRALATAAIGSLQATGHSGDQIMLGELAPLGQKTGRPASRNTAPVTFWQEMLCLGSNG
                     RPLTGSARKARGCPAKFPRFQVNAVSHHPYNRGGASSPLTRGNPGDITLATISRLQSV
                     LTQGSRARRIPSNLPIYYTEFGFQTNPPDRTFGMSLSRQAEYINESDYIAYRNSRIAS
                     VAQYELNDEPNLASFQTGLRFEAGKAKPSYAAYRLPIYVVRHGSKVGVFGQVRPAPDG
                     SSDTVLIENRSGRTWNVVKTIKVSSQKGYFLVNTKYRSGSWRLSWTPSGGGSTVFSRS
                     AAARSR"
     gene            complement(18724..19500)
                     /locus_tag="VF752_17190"
     CDS             complement(18724..19500)
                     /locus_tag="VF752_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8857332.1"
                     /translation="MAQAPVVIVETSGAVGIARLNRPDARNALSPELFAELGEAIQSY
                     DRDDAIGCIVIAGSDEWFAAGADIKSMRDRAWQDALAQPSSEFWPKLAGCRTPLIAAV
                     SGYALGGGCELALGCDMIVASETAEFGQPEIMLGIIPGGGGTQRLTRVIGKQRAMELV
                     LTGRRITAQEAYDWGLVNRLAAKRKWLEDAVELAEVVARRAPIAVKLGKQAVLAADET
                     PLGAGLAHERRLFELALATEDRVEGMSAFIEKRKPQFRGR"
     gene            complement(19500..20657)
                     /locus_tag="VF752_17195"
     CDS             complement(19500..20657)
                     /locus_tag="VF752_17195"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8857333.1"
                     /translation="MIDLELTDEQRLIRETARDFTDREIIPVARDNDRNERFDEELVK
                     KIADMGFLGAIVPEEYGGRGLDYMSYGLIVEQIGRGDSSARTVVSVQTSLVCSAIVRW
                     GSEEQKHQWLPKLCSGEILGCFGLTEPDTGSDAASLKTRATKTADGWKISGQKQWISN
                     GTKAKLALIFAQTDPEQKHRGLAAFLVPTDSPGFSSSPVHGKLGLRASDTAELSLDEV
                     EVGDEALMGEIGDGFKVAMSALDSGRFSVASGCVGICQGCVDASVAYSKERTQFDRPI
                     ASFQLVQELIADMVVDTEAARMLCWRAAWLKDSGKPSTTETSVAKLYATEAAVRSANA
                     AIQVHGGSGYVDDYPVERYLRDARVTTLYEGTSQIQKLIIGRAATGINAMT"
     gene            complement(20670..21488)
                     /locus_tag="VF752_17200"
     CDS             complement(20670..21488)
                     /locus_tag="VF752_17200"
                     /inference="COORDINATES: protein motif:HMM:NF005878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8857334.1"
                     /translation="MTGRVVGITGGARGIGAAIGEALSRDGARVALGDIDGEAARQTA
                     ERIGGGAIGLELDVTSTESFSAFLDAVENELGPLDVLVNNAGIMWVGPFSDEPGSAEA
                     RQFAVNTHGTMRGMKLAIPRMLRRGRGHVVNVASMASKVTPAGEATYTASKHAVYGYT
                     AAVRAELEKTGIDITIVMPAVVETELAKGTSSGRTKRLTPEDVGRAVAAAVRKPRFEV
                     YVPASLSVVSRIYAALPIRLRDAMARAIVPDQVKETDRAVRAAYEDASVAARDE"
     gene            complement(21485..21859)
                     /locus_tag="VF752_17205"
     CDS             complement(21485..21859)
                     /locus_tag="VF752_17205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8857335.1"
                     /translation="MDWRLELVQVPVSDVDRAKAFYTEKLRFNADVDVSPQEGLRFVQ
                     LTPPGSPASIAIGTGLTQMEPGSIEGLQVVVADADAARAELVERGVEVSEVQEFPWGR
                     FVFFSDPDGNRWALQQITQRAS"
     gene            complement(22097..23296)
                     /locus_tag="VF752_17210"
     CDS             complement(22097..23296)
                     /locus_tag="VF752_17210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX8857336.1"
                     /translation="MPDALIIDAVRTPIGRYGGALSTVRPDDLAARVIEAVVERTGLP
                     GGDIADVYLGCTNAAGEDNRNVARMSALLAGLPVEVPGVTVNRLCASGLEAVNQAARA
                     IMVGDGDLMLAGGVESMSRSPFVMLKPERSLPRGDMQLVDTTIGWRFVNPRMRENYST
                     ESMGETAENVAERYSISREDQDRFALQSHERAVAAAEAGRFDDEIVTVDAPQPKGPPV
                     TVHSDEGPRPETTLERLAGLRAVFREGGSVTAGNSSQINDGAACLVLASPDRAEQLGR
                     EPLARIVSTGVAGVDPGYMGVGPIPATRQALERAGLTIDDIDLVELNEAFASQSLACA
                     RELGIPQEKLNVNGGAIALGHPLGCSGARLLTTLVHEMRRRGAKLGLATMCIGVGQGL
                     ATIVEAV"
     gene            23319..23648
                     /locus_tag="VF752_17215"
     CDS             23319..23648
                     /locus_tag="VF752_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857337.1"
                     /translation="MEFALAATGVIVVLAIVFILIGHFYPGSGADVLDWRPTRSFDEE
                     ARLELEDIDQMLEAQNERRRRSGRPELSEAGIQAEVDSERRAMLERGERYRGSEEGPT
                     PPPQSDG"
     gene            complement(23620..24555)
                     /locus_tag="VF752_17220"
     CDS             complement(23620..24555)
                     /locus_tag="VF752_17220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8857338.1"
                     /translation="MTRTPRHPGLHDAKGRELEHQLLDVDGVATYTVDAGEGSPILFV
                     HGYGDTADGWRRVVPGLLETNRVIAIDVPPFGRSGRISERELFDFYAGYFPALLDRLE
                     IKSATLVGHSLGGAIALRFALANPKRVARLGLVAPAGLGKKPPWWWRPIAGNGVAWRA
                     ALAIPSPLTPLIIREGMSRFLDLRLFHDSRALRQDIRHLVDLHSTTKDFGALLEAGRA
                     CIASYTGTLLEDSKAITCPKFMVWGRHDGLVPVSEGLAFQQAHPDADVHILERCGHYP
                     QIEMPGRFNRLLRAWIADTAGACDKPLTRRTVEAA"
     gene            24694..25185
                     /locus_tag="VF752_17225"
     CDS             24694..25185
                     /locus_tag="VF752_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857339.1"
                     /translation="MARVLVVGCGCRGLELARALAADGHAVRGTTRRQERLPEIEAAG
                     AQAALADPDRLATMLPHIDGVAVMCWIMGSASGEPDHVAALHGPRLESMVETLVDTHV
                     RGVVYEAAGSVDQALLEHGAASVRMAGARFHMPVEVVDTDPRERTDWLEAMRAAVARV
                     LAA"
     gene            complement(25182..>25450)
                     /locus_tag="VF752_17230"
     CDS             complement(25182..>25450)
                     /locus_tag="VF752_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857340.1"
                     /translation="VVAAAPDEVIAAEPPDVGEPPAPSAPPPPPVIAVPVGPVPGPLE
                     PPARTRREIAVDTSWHALFAVAVVLALAAIFLTVAGATGLIERI"
BASE COUNT         3927 a         8956 c         8772 g         3795 t
ORIGIN      
        1 gtcgtcggcg agctgatgca gcggcgagtc tacgtgggcg acgcaaccgc cgtgggctcc
       61 ggccgcggcg cgcgcacgcg agccgcgaga gcggagcagg cgagcgcgcc gcccgtcgcg
      121 agcagtggca ccgagggctc caggtagcgc agcacgaact ccgaggtggc cgccgcgccg
      181 accagcatcg cgaggccggc gcccgtgaag agaaacgtct cgcgccgccc gtgtaccgcg
      241 cccggcagcg cgacgaccgc gagcgatgcg agcgagagca gcgccagcag ccagcgcggc
      301 gcgtggacga gccgcacgta gtcgcgcgcg gcgcttgtgg catgcggcgg ctcgtacctc
      361 gggaaccagc ggtcgcggac gacggggttc agcaccgtcg cgagctcgag cgcgcggtcg
      421 gaggtcccgc gcgagcgctc gcccggcgtg aggaagcgca ggaagtccga ccccgccgcg
      481 cgcgcgtagt cgagcggctg cgcgcggatc tggtcggtcg cgaagccgcg cagcacccgg
      541 ttggagtgag ccagcctcga cgggtccgcg ctgaatccgt ggaacaggcg gttcgcgggc
      601 gacgcggggt cgaacacgta gtacagcgcg cccgcgcccg gcttcggggg gacgcagagc
      661 ggccgcgcct cacgctcgac gctcgcgctg cggcaatcca cgaactcgcc cacacgcccg
      721 tagagctgcc agcccgacgc cgacgtgaag ccgtagctgt cctgcttggc gtggatagag
      781 cacgagcggc agcgcgcacg cggcggccgc cacggcgatc gcgcgccacg ccgggcgcgt
      841 ccacaccagg tacgcgatcc aaaccggcac cgcgaagagc gcgaccggtc tgatgagcac
      901 ggcggccgca agcagcccgc cgcttcccgc gagccgggcg atgccgcgct cccccggcca
      961 tgccgcgagc gcgagcgcgg ccacgagcac gagcgtgaac agcgcctctg cgagcaggtg
     1021 ctgctcgagc gcgatggccc agccgtcgag aagcacgagc gcggctgcgg cggccgcgag
     1081 cccgcgccgc aatccgagcg ccacgagtag cgcgtagagc agcacgccgg tcgccacgcc
     1141 catcaggtgc tgcacgagcg tgagcgcagc gaggctgcgg cccggcagcg cgagcacgtg
     1201 aaggaagagc ggatagccgg cggggtggag ggggtcgctg aggtactggc ccttgtaggc
     1261 ggcgtccgcg tacgcccacg agtcggtgta caagagcccg ggccacgcgc cgagccaaac
     1321 gagcagccgc gccgcgagac cgagggcgag tactcccgcg aaccatttgt gcgcaccgat
     1381 gcgcgcccct gccatcaggc gcctcggtct gccagcgggg ggcgcgaggc gcgtcagtcg
     1441 cttctacgcg ggcgcggcag tcgcgaagtc ctcgaccgta agcgagcggg cgccgatctc
     1501 ggcgctcgcg agccgcggcg gatcggcgcc cgcacgcccg catgtcagag acgacaccag
     1561 cacggcctcg tcgacgacct ccgccagcgc gccgtccggg atcgcggcga cttgctgcgg
     1621 cgcgtgcaat gcgcgccgga ccaggccgct gagcagacca gcggtgaacg cgtcgcccgc
     1681 cccgatggtg tcgacgacct caaccgcgcg tcctggccgg tgcagcgcct cggttccctc
     1741 gcgaaaagcg gtcgcaccgt ttggtccctc cgtgacaacg acgagttccg cgccgaggtc
     1801 gttccagcgc gtggcgatgt cgtccacaga gcgcgacggg tagagccact cgatgtcctc
     1861 gcggctcgcc ttcacgacgt gcgatctgcg cacactccgc tcgaccagcc gcctgccccg
     1921 tgtgggcgcg tcgagcagat gcggccggat gttggggtcg tagctgacca ggactccgtt
     1981 cctcgcgcgc gcctcggcga ccagctggtc gatgcgcttg ctccccggcg gcgtccagga
     2041 ggcgatcgag ccgaagtgga ggaccgccgt atcggtgggg cgcttgcgca gctctgcggt
     2101 gctccactgc cagtccgccg tgccctcgct gtagaactcg tagctcgcct gccccggcat
     2161 gtccatcgac accgcggcga gcgtcgtcag ctccgccgcg cgggccgaag cgctgaggtc
     2221 cacgccctct ccggtggcgg tttcgcggag caggcgcccg aagccgtgat cggagaggcg
     2281 cgccatgagc gccgtccggt tgccgaggcg cgcgagcgcg accgccacat taaaggggct
     2341 acctccggga tgcgcgcggt attggcccgg acctgttggc acgaggtcga tgagggcctc
     2401 gccgatgacg gtcagtcggg ctgagctcac actgcgcctc cagcgatctc cacattgatg
     2461 cccgtcaggt acgacgagcg atcgccggcg aggaagcaga tcacatcggc cacctcggcg
     2521 ggcgagccgt agcggcgcag cgggaccaat ccgatcatct gacgggccac ctcgtctgga
     2581 tcggtggagt agtactggct cccgaccgcc gcctgctgtg cgacctggtt ctcccacatc
     2641 gcgccagggc cgatgaacgc cggcgagacg gcgttcacgc ggatgccgtg cggcgccaag
     2701 tcccggctgg cgctcttcgc caagccgatc accgcggcct tcgaggccga gtatgcgggc
     2761 atgttcggga cgccgctcac tcccgccatg gatgcgatcg cgacgatcga gccgcccgtg
     2821 ccggcaccga tcatcagctc cgcggtcgcc ttcaaggtgt tgaagacgcc catcacgttg
     2881 acgtcgatga cgcgccgggc cacgtcgacg tcgtagtcct gcacgggcgc gaagtcgccc
     2941 tggagacccg cgcccgccag gaccgccgtc ggcacgccgt gacgttccgc gagcatgccg
     3001 acctcgcgac cgaccacggc gtgatcggtc acgtcaaatg tggaggcgac cacctcggcg
     3061 cctgaggcca cgcatgcgtc gcgcgtggcg tcgagccgcg cagcttgccc cgggtgatcg
     3121 ctgacgaaga gggaccagcc gtcctccgcc agcgcgacgc acgccgcgcg tccgaggtca
     3181 ccggccgcgc cggtgacgag cgcgtgacgg ctcaccttga tcgatcaccg cagactcgcg
     3241 tgcgtcgtcg acgtgacccg cgcgccgtcg ccggcgaaca ggtgcgttgt cccgacgagc
     3301 tcgagcccga cgttgtcgcc gggctcgccg tggaacggcg gcgacatctt cgcgaccaac
     3361 gggatgctct cgccctggtg gtcgatagcg acatggacga acacctctga gccgaggtcc
     3421 tcgaccgtcc ggatgtgcgc gcgtagaggc ccgtcgccga cccgaagcgc ctccgggcga
     3481 aggcccactg tgatctcacc atccgcgtcg gcgacggcgc cgttggcgga tgggtccagc
     3541 tcgatctgag cgccgccgat cctgagcggc cggttcccgt cggcgttcac gaggttcatg
     3601 gccggcgagc cgatgaagcc cgcgacgaac aggttgttcg gctcgtggta cagcgtgccg
     3661 ggtgcgtcgc attgctgcag gactccgtcc ctgagcacag cgacccggtc gcccattgtc
     3721 atggcctcca cttggtcgtg agtcacgtag acggtcgtga ccccgagccg gttctgcagc
     3781 tccaccacct cggtgcgtgt ctgcacgcgc agcttcgcat cgaggttcga gagcggctcg
     3841 tccatcaggt agacggccgg ttgccggacg atggcccgac ccatcgccac gcgctggcgc
     3901 tggccgccgg acagctgctt cggcttgcgg tcgaggaacg gcacgaggtc gaggatgcgc
     3961 gccgtctcgc gcacgcgggc ggtgcgctcc tccttcggca cgccgtgctg cttgagcgcg
     4021 aagcccatgt tctccgccac ggtgagctgt ggatagagcg cgtagttctg gaagaccatc
     4081 gcgatgtcac gatcgcgcgg ccgcacgtcg ctcacgtccc ggtcgccgat gtaaaccgcg
     4141 cccgcatcga gcggctcgag tccagccagc atccggagtg ccgtggactt ccccgatccc
     4201 gacggcccga cgaggacgag cagctcgccg tcaccgacgt cgagttccag gtcgtcgacc
     4261 gccgggacgt ccgtcccctc gtaggtgcgt gtcgcatgtt cgtatctcac tgcggtcatc
     4321 gcgggttcct cacttgattg cacccatggc gaggccacgg atcatgcgtt tctgggcgat
     4381 ccatccggcg agcacgacag ggatgcacgc aagcaccgac gcggcggaca gcttcgcgag
     4441 gaagtcgccg cgggtgctga tgttcgtcgt cacccagatc ggcacagtgg agccctggac
     4501 cgggttgatc tgaacggcgt agaagaactc gttccaggca aagatcacgc acagcagcgc
     4561 ggtggcggcg atgccggggg cggcgatcgg gaggatgatc gaggtgagct ggcccttgag
     4621 ccccgcgccg tcgatctctg ccgcctcgat gagctcgcgc gggatctcgg caaagaacga
     4681 gcgcagcatc cagaccgcca acggcaggtt gatcgcggtg tacaggatga tcagtgacat
     4741 cctcgtgttc agcaggtgga agttccgagc gaggatccag atcggcagga tcgacgcgac
     4801 gatcggcagg aacttcgtcg agatgaagaa gaagagcacg tctcgccact tgtggaccgg
     4861 cttgaccgcg agggcgtacg ccgccgggat cgccagcagc atcacgatcg cgaccgagac
     4921 caacacgacg accgccgagt tcgtgaaggc ctcgccgaag ttgagcactc cgccggcgct
     4981 cctcgttacg tccctgtagc ggtcgagcgt cggatggaag atgaacttcg ggctcgtgtt
     5041 cgcgtcctgc tcggtcttga acgagttgag caccatccag aagaccggga agaagaacac
     5101 gagcccgact atccaggcga gcaggccgac ggagcggttg acccagcggc tttcaccaac
     5161 caccctgggc gccgggactg cgctcatgcg atctcctcac ctttcaagag attggacagg
     5221 acgcgcagcg agatcgtcgc gatgacgatc gacgccagca cgaccacgat gctgtacgag
     5281 gacgcctgcc cgaactccca gccgccgccg atcgatttct ggaacacgaa gtacggcacg
     5341 ttcgtcgagc cgggcccacc gccggtcatg actgcgacca ggtcgtacac ctggatcagg
     5401 tagatcgtgc cgagcaggat gccgagctcc atgtacgggc gaaggtggct gagcgtgagc
     5461 tgccggaaga tgccccaggg cgttgccccg tcgaccttcg ccgcttcgag cacgtcggcc
     5521 ggctggctct gaagccccgc gagcatgatc agcatcatga acggggtcca ctgccagatc
     5581 agcgtgaccg cgatcgccgt ggtcggatgg cgcgcgacga agtcgaccgg cgtgccgccg
     5641 agcttgtcga tcccccagtt gatgacgccg tagaggtcgt tgaacatctg gttcttccag
     5701 atgagcccga ccaccacagg catcaggagg aacggcgtga tcagcagcgt gcgcaccacg
     5761 ccccgaccga agaacttgcg gtcgagcagg agcgcgaacg cggtcccgag cacgagagcg
     5821 ccgaacaccg ggatcacggc cagccggaca ctcacccata ccgcgtcgcg gaagaagtgg
     5881 tcgctgaaca ggtgtccgta gttgtccagg ccgatgaact tgcgcggccc tggaggtacg
     5941 acgtgccagt ccgtgaggct gtaccagatg ctcatcacga acgggacctg cgtgaccacg
     6001 atcgtgaaga tcagcgccgg caggagtggg agccgccggc gccacgactc gctcaggcca
     6061 ggaggggtaa gccttcgctg cctcggctgg gaggcggtcg tggcgccggc ggctgtgctc
     6121 atcgcgacat gggcatcgtt gtctccgagc agagcatggt cacgggcggt gggacggcgt
     6181 cattgcgcca cctgcgacgc gacctgctga cagttgctca acgcggaatc gatgctggac
     6241 ctgccggcta tcacggctga gaactgctgg gtacattgat tcccgacgtc ctggaactgc
     6301 gggatcccga cgtattgcac gccggggagg ccaggccgtt tcgtcgtgcc cggattcgtg
     6361 atcggggccg atgagatcga gtcgagcgtt ggcttggcga acgccgccgc cgccttcttg
     6421 tactgcggaa tgtcgtacag cgacttgcgc gcgcccggcg gcagcgccgc ccacccacct
     6481 gggatcttcg cgccggcttc cttcaggtac tgcgggccgg tcgcgaactc gatgtacttc
     6541 catgcgacgt cttgcttgct gctcgtcttc gggatcgaca gcgcccacga ccacagccag
     6601 cccgcgtcct tggtctcctt gaccggcgcc gcagcgtagc cgttcttgcc cttcaccggg
     6661 ctgtcgctcg cctcgaggtt gctggctgcc acggtggcgt cgtaccacat ggccaccttg
     6721 ctgtccttgt actgcgtgag gcactcattg aagctcgagt tggcggcatc cttctccccg
     6781 gcgtcgtgga tcaggccggt gtaaaactgg agcgcctgct tgaactgcgg ctgatttacc
     6841 atcgccttgt cgacggagcc gtccggcttc gccgaccacc acgtgccacc gaacgtgttc
     6901 aggaccgtgg tgaacgacgc gcctagatcg ccccatccag gcttgccgcg caggcagatg
     6961 cccgccatct tggagctgtt gaccttgcgc gcgatggctg caacctggtc ccacgtcggc
     7021 ctcggcggta ccgtgatgcc tgcctttttc atcacgtcct tgcggtacat caggaacgac
     7081 gactcaccgt agaacggcgc ggcgtacagc ttcccgtccg cggacagcgc gttgcgcacc
     7141 gccgggatga tgtcgttgaa ctgatacgcc gagctgttct gggccttcgg cgtgaggtcg
     7201 acgaggttgc ccgcttgacc gaactggggc gcctcgtatg gaccgatcat cgccacgtcg
     7261 aactgcctgc cgccggcggc gacgtcacgg gtcgtgacct gacgcagcgt gccctcgtcc
     7321 aagaccgtgt agttcacctt gatcccggtc ttcttggtga acagcgaggg cgtcagcttc
     7381 gcgatgtcct tcatctgcgg gttgtcaacg atcgcgacgt tgatcgattt gctgctggag
     7441 ttgttgctcg agccgccacc gccaccgcca ccgccgcagc cggtcgcgac gacgcccgcg
     7501 acgccggcga cggccagcac ggcggccgcc gaccgagcac cggtcatccc tctcatctca
     7561 cttctcctcc tgcttcgccg gagggcaagg gattccctcc gcagtgaggg acttataccg
     7621 ttgggttgtg tgggatgtca agggaaaatc acgcccattc ccacgtaacc aaatgcttgt
     7681 cggatggatt ctcctgcgtt tatgatgcca gcctgtggtt ggcgagcctc cgatcagcct
     7741 caatcaggcg cacttggcgg agattgcaag tcggggcgtg ccggttcccg tctacgaccg
     7801 cgcgcggctt gcggcgcgca tcgtccacat cggtgtcggc ggcttccacc gcgcgcattt
     7861 cgcgctctac acgcacgagc tggctgaggc gggaagcgac tggggcatcg tcggtctcgg
     7921 gctgctccca cgggacgccg agatggccgc cgcgctcacc ggccaggact ttctctacac
     7981 gctgatcgag aaaggcaacg gtgagccgtg cgctgaagtg atcggctcga tcaccggata
     8041 tgtgcatgcg cccgacgggc gcgatggcgc cgtggacgag ctgatcgcct cgccaaccac
     8101 ggcgatcctt tcgctcacgg tcacggaggc cggctactcg gacgaggccg aggcgcggca
     8161 ctcgttcgat cgcatcgcca cggcgctggc gactcgccgc gcgcgctcgg cagggccgct
     8221 cacgatcctc agcttcgaca acatccccgg caacggcgac gcggcgcggc acgcgaccct
     8281 ctccgccgcg acgcggcgag acggcgagct cgccgcttgg gtgcaggaga actgcacgtt
     8341 cccgaactcg atggtggacc ggatcacgcc cgtgacggcc gacagcgacc gcacgtggct
     8401 gctcgagaac gcgggcatcg aggatcgctg gcccgtagtg gccgagccgt ttcgccagtg
     8461 ggttgtggag gacgagttcg cggcgggccg gccttcgtgg gaccaggcgg gcgcgatctt
     8521 cacggatcgc gtgcacgact gggagcggta caagctccgc ttcctcaacg ccgggcactc
     8581 gtccatggcg tacctttcag ctctggccgg catcacgttc gtccatgagg cgatggcgat
     8641 tcgagccata cagacgttcc tcgaagccct cctttaccgc gaggcaatgc cgacagtcgc
     8701 cgcgatcccc ggacatccga gagaggacta cgtcgcctcg gtcctggagc gattcggcaa
     8761 cagcggcgtc cgcgaccaga tcgcacgact gtgcatcgac gggagcgcga agttcccgac
     8821 cttcctcatc ccgacgatcg tccggcagct cgaggccgaa ggcccgatcg accgtgcggc
     8881 gaccgcgctt gcgggctggg cgcggtacct cggcgtgatc gacccggagc accaggcgtt
     8941 cgatgccagt ggggatacgg cgcggcggta cgccgcggcc gccacgggcg acccgctcgc
     9001 gttcctcgac tacaaagccg tcttcccgcc ggagctgcgc ggctcgacgc gcttccgtac
     9061 tgcgttcgcg gagagctacc ggcggatcac ggagcacgga ccgatctcag cgatggaacc
     9121 tgaaacgtcc gggcagcgca cgggagacgc acgatgacga tctccccgcc cgaaggccct
     9181 cggcttccgc acgatcgcca gcacgcgatc tacatgctcg ccctgcgtca gggctcggtc
     9241 gacgttgcgg acctggcgcg ccgctacggc gtgacgaccg agacgatccg gcgcgacctg
     9301 tccgatctcc aaaagcggcg tctgcttcgc cgggtccacg gcggggcggt ccccgtcgag
     9361 cggatgaacc acgagccgat ggtcgacgcg cgcgacatgg tgaacgccga ggagaagctc
     9421 cgcatcgcga ccatggccgt gcaggaggtg ccggaacgcg gctcggtgat catcgactcg
     9481 gggtcgaccg ggcagcgcct cgccgacgtc ttcccggtcg agcgcgacat ccacgtggtc
     9541 acgaactcgc tgatcaccgc gctcaccttg agccgccgcg gtctgcgtga cctcacggtg
     9601 ctcggtggcg gtgttcgcac caagcggctc gcgatggtgg atgacaccac tcgccgggag
     9661 ctcgagcaca tggccatcga tgtgctgttc atcagctgcg acggcctgtc gttcaagcac
     9721 gggctcacga cgccgtaccg ggaagagcac acgatcaagc gagcgatgat cgagcgggcc
     9781 agtcgcgtcg tcgccctggt cgacagctcg aagttcggca acgtgcagat gttcggcttc
     9841 gcatcgttcg acgagatcga tgtgctggtg accgacacgg aggccgacgc ggacgcggtc
     9901 gacatcttgt cgacccacgg catcatcgtc caccgtgcgt gacagcggcg cggctcggga
     9961 gcccgccgag ctggaacgga tcgccgcgcg cgcggcgcag gcggccgcgg ggctcgtaac
    10021 ggccgcctac gggagccccg acccggtggg gcgcaagtcg tcgccgaccg acgtcgtcac
    10081 gcagacggac ctgagcgccg aggaactcgt cagaggagtg ctgcgggagg agacccccgc
    10141 cgccggcgtg ctcggcgagg aaggcgggaa gacggcgcgc ggcgctcgcc tgcagtgggt
    10201 gatcgaccct ctcgacggca cgatcaactt cctgtacggc gtgccgctct tcgcggtgag
    10261 catcgcggcg gcgctcgacg gcgaggtggt cgcgggcgcg gtgatcgacg tgctgcgcga
    10321 cgagctcttc tcggcgcatc gcggcggggg agcccgtctc aacgggaagg cgatctcagt
    10381 ctcggcgtgc gcgtcgctgt cggaggcgct ggttgcgacg ggcttctcgt accaggcgga
    10441 gcttcgctcg gtccagggcg aggtggccca gcgcctgctc tcccgcgcgc gggacctacg
    10501 ctgcttcggc tcggcggcgc tcgagctctg ctgggtggcg tgcgctcggc tcgacggcta
    10561 cttccagcgc gacaccgaga tctgggaccg cgccgccggt ggcctgatcg cacaggaggc
    10621 gggcgcgcgc gtggagatgc cgtgccccga gaacgacgac ctcgtgatcg ccgctgccca
    10681 cggcgtgttc gacgacctct gggccgccgt tcacttcgcc gcggcctgaa gcgccggcga
    10741 ctcgtaccgc gtccaaacct ggaacagcgc ggctcgcgga acgccttcgg ccatgcgcgc
    10801 ggccgcttgg atcgcctgcc agcgggccag cagttcgtca tcgatctcga gggccgtccg
    10861 caaacgctcg aggtatccct cggtcagtgc agcgagcgtg gcggcgtcgg atcccgggag
    10921 gtggcggggg ccgctggtgc cgtcgccgag cagtgtgacc aacgtgcggg cgacgtcggc
    10981 gaccggatcc ccgcgcgatg cgtcgaacca gtcgacgatc atcgggcctc ggtcgcagag
    11041 gatcacgttg ctcggatgga gatcgccgtg gcacagccgc ggcgcgttgg ccggcgacgg
    11101 caagagctcc agcgcgcgcc tcacggacac gtccaccgtc gcggcggacc atcggatctt
    11161 gctgacgagc cgatcgcgct ggtttggcag cgtgacgggc ggcacgagct cgaacagccc
    11221 gcactgggtg tcggcgagca tccggcccag ctcgcggctg gattgcggcc ggtcgatcac
    11281 gtgctgccac atcgacgttc cctcggcccg ctcccacacg ctggccgcgc ggccggagat
    11341 cggctcaatg ccaaggaggc gcggcacggg cgcgcccgcc tcacgtgccg cctcggcgta
    11401 ctccgcctcg aattcgatcc agtcggtggg ggtggacgcg tgcggcacct tgatgacgac
    11461 accacgtcca taggcgtgga tcgaagagcg cgtgccacgc gcgacgaggt caccgagcag
    11521 tctggtcacc gtgcaagtcc ccccggaaca cgtccagctt ggacccaacg taccacccgt
    11581 gcttgcccac cggctcttgg cggacgggta ctgagcacgg gatgtcccag tcgatcgtgg
    11641 acctctccct acgcctgtac gacgcgctca accggcgcga ctgggatgcc tttcgcgaac
    11701 tgctggaaga cgaggtggag atcgaatcac gcctcgtcgt gatggaaggc ccttaccacg
    11761 gccacgcggg cgcgcgtcgc tggtggaacg actttctcgg cgcctttccg gactacgagg
    11821 tcgaggtcca ggagctgcgc gacctcggcg acgtgacgct cgcgttcatc cgcggctggg
    11881 ggcacggcgt cgcgagcgcc acgccgatca tcgacccctt ctggcatccg gtcagatggg
    11941 tcgacggcaa atgcgtgtgg tggcgcaact gcgtcacgga ggaggaagag cttcaggcga
    12001 tcggggcgct gccttaagaa ctcgagacag cgctccgcat gcggcggccc aatgcgacta
    12061 tgcgcgcgag gtggccgcag tacggggcgc ctgggcagcg ggcgaacgac agcgcgaccg
    12121 tctccaccgc ctcgtcctcg ggcttgcgcc ggcggccggg aagcagcacc agcgccaccg
    12181 cgacgccgag cgcgcagagg atcgccgcgg cgaggaacgc gcctttgaag ccgtgggtga
    12241 gcgcaacggg cagcgacgag ccggcggcga gagagctcgc gacgcggctg ttcgaaaccg
    12301 acgagaggat cgcgatgccc agcgagccac cgacctgctg agtcgtgttg agcaggcccg
    12361 atgccagccc ggagtcggcc gccgccacgc cgttcgtggc ggcgatcgtg atcggcacga
    12421 aggacattcc gagaccggtc cccaggacga gcatggccgg cagtacgtgg ctggcgtagt
    12481 cgctgtggcc cgacactccg gtgagcagca cgaagccggc tgtggagatg agcagccccg
    12541 cggccagcac cggctttggc gtgaagcggt cgacgaagcg cgacgcgagc gccgacgctc
    12601 cgacgagcgt caggctcaac ggcaggtagg cgacgccggt cttgagcgcg ctgtagccca
    12661 aaacctgctg caggtagagg gtgcagaaga agaacactcc gaagagcgcc gccgcgacta
    12721 ccagcatcgt cgcgtcggag gcggcgaggc cgcggttcga gaagatccgc agagggacga
    12781 gcggttccgg ctgccggtgt tcgatgacca cgaacgcgac gatgagcgca gcggcgatag
    12841 cgaagcccgc cagagtgcgt ccggacgtcc aaccccagct gtcggccttg atgagcgtgt
    12901 agacaagggc gatggtgccg agtgtgatcg cgagagcgcc ctcggcgtcg tagccgccct
    12961 gcgtgccgga ctcggtgcgg ctctccggga tgatccgcgg cgccagcgcc aataccaccg
    13021 cgccgatcgg cacgttgatg aagaacaccc agcgccaggt cagggcctcg acgatcaggc
    13081 cgccaagcag tagcccaacc gcgccgccag ccccggcgat cgcgccccac accgcgagcg
    13141 cgcggttgcg ctccggtccc tcggcgaagg tcgtgaggat gatcgacagc gccgccggtg
    13201 agacgagcgc gccgcccagt ccctgcgcgg cccgagcgcc gagcagcatg ccctcggact
    13261 gcgagacccc gcagacgagc gaggcgcctg cgaaaagggc gatcccggcg accaacaggc
    13321 gcctccgccc gagccggtcg gcgagccggc cgcccagcag gagaaaaccg ccgaagacca
    13381 gggtgtaggc gttcagcacc caggagaggc tctgctcgga gaaccccacg tcgcgcttga
    13441 tcgtcgggag cgcgacgttc accaccgtcg catccagcac gaccatcacc tgggccacgg
    13501 cgacgagcgc cagtacggcg ccggggtcga cgcgcgacac ggggcgaggc gcgggagagg
    13561 cggcggcgga agacatccag gccagtccta tggcgacagt aacagggccc cgatgccggc
    13621 agacgaactg cccgcgcgct acaacgaggc tgctgcccgc accagccgcg cgttgctgcg
    13681 cgccgtgctg ccgtccggca tcacgagcgt gtcctcgagc ccgaccctga tcgagtggcg
    13741 gcggtcgcgg gccgcctcga gcaccgccca ggtcgcgccg ccgatgccgt ggtggagttg
    13801 ctcgatgccg gtggggtaga ccacgcgctc gatctccccg gcgagggcga ccgcgctggc
    13861 cgagtcgtct ccctcgacct ccacgagcgc gcggcggcag cgcacggcga actcgctctc
    13921 cacgagcttc tccgcatcgg ccaccgacgc caggcccgcc tcgacctcga tgcctcggcg
    13981 gagcgccaac tcgatcaact cgagcgcgcc gtcctcggac aggttcacgg acaccatgtc
    14041 cggcagctcg tgccagctct ccaccgcggc gcgcgtggcc gtggcgctgc cctcgatcca
    14101 gatgccggtg ctcagcccga gctcggcagt ggcgaccacg ctccgcacgg cgcgcagcgc
    14161 ctccgcgcag atgccggggt cgagcgtctc gatgccgttc tcgtcgcgcg ggtggaagtg
    14221 catcgagcgc gcgcccgccg agcggcacgc ccgcgcgtcg gtcgctagct gcgcgggagt
    14281 gatcggtact gcctcgtgct cgagccgcga gcgggtgccg ttcaggcagg cctgaagcac
    14341 cacgggctac gcgctcagca cgatcttcag cgcctcgtgc cgggcgtagg tctcgtaggc
    14401 ctggggcgcc tgctcgagct tcatgtgatg agtgacgaga ggcgtcggat cgaggcgccc
    14461 ggccgccatc agcgcgagca cggagtcgag atggcccatc acgttcgcgt ggcccgtgag
    14521 cacggtgagg ctcttgagcc acgccatgcc cacgttcact tcctcgcgct cggcgtgcac
    14581 tccgacaagt gagacggtgc ccgccttgcg cgcgagccgt agcgcgaggt cgagcgtctg
    14641 gccggtcccc accgcctcga tcgcgatgtc cacgccgcgg ccgtcggtgg ccgccttggc
    14701 cgccgcccgc gggtcctcct cggtcgcgtg gacgggctcg gcgccgagcg tgcgtgcggt
    14761 cgcgagccgg tcgggcacag tgtcgatccc gatcacgcgc gcggcacccg ccacccgcgc
    14821 ggccatcacg gcgcagagcc cgacgggacc gagcccgatc acggcgcagg tgtcgccggg
    14881 ctcgaggccg gcggagacga cggcgtggta gcccgtaccc atgacgtcgc cggcgaacag
    14941 cgcggcgtcg tccgagagcc cgtcgggcac gcgccgcagc gtcatgttcg cgcgcggcac
    15001 gagcacgcgc tcggcctgag ttccctgaag cgagccgagc agcttgccgt gcccgaacac
    15061 ccgtgcttca tcgcatttct ggaacacgcc gcgccggcag aagaagcagg ttccgcaggc
    15121 cgtgtggaag gtgccgagca cgcggtcgcc gaccgacacg cttgtcacgg cgtcacccgc
    15181 ctcgaccacc ctgcccacgt actcgtgccc gatcgtgaag ccaggctcga cgcccactcg
    15241 cccgtggaag atgtgcaggt ctgagccgca gatgcccgac gcctcgaccg cgactaccgc
    15301 gtcgtccggc gcctgcggct cgggctcggg ccgctcctcg accgccaccc ggccgggctc
    15361 ctggaacgtg accgcgagca tccgcctcga ccctataggt cctcgctcgc cgccacggaa
    15421 cgtctcagga gtggcgccgc gcggtagcgc tcctcccgcc gctcctcgta cagcccgtcg
    15481 atcgtcgcga gcacgtggtc aagcccgatg gcgcggctcc actcgaccgg cccgcgcggg
    15541 tggttgagcc cgagcgtcac accggcgtcc acgtcctctg gggttccgac gccttcaccg
    15601 atcgcgaacg ccgcctcgtt cgccagctgg cacacgatgc gaccgagcac gaggcccggc
    15661 gcgtcgcaga cccactcatg gtggtagccg agcgcggtgg cgaactcctc ggcgcgctcc
    15721 tgggtgagcg cgggcgtgga cggcagccga gtgagctcca tcagtttcga gtcaccgagc
    15781 ggcggcagaa gatggaaacc acatgcttcc ggctgtccgc gctgcgcgag gctgaggtcg
    15841 gcgcacagcg cgagcaacgg cggccctgtc ggcgagtcgc cgagcaggtc ctcgtccgcc
    15901 accggtgcgg gcgactccgc atccacgagc agccacagct cctcggcgtg aaactcggac
    15961 gcctcgcgcg cgtcgaagcc ggcgtcgcgc gcgcgctcgc gaagcgccga cgcgagcctg
    16021 ccgctgccgt cgatcgccac agggcggccg tcgcctccgc cgagcggggg cgggtcggcg
    16081 tcgtcgggcc ggtgcgcacc gtcgccatac tcgtaccagc cgcgaccggt cttgcgcccc
    16141 agctgcccgg ccgcgacctt ctgcgcctgc agcatgttcg gcttccagcg cggttcgccg
    16201 aagctctgag cggtgaacga ctgcgcgacc tcgaagccca catcgatccc cacgaggtcc
    16261 atcagctcga acggtcccat gcggaagccg ccgccgagcc ggcagatccg gtctatctgc
    16321 tcgaccgttg cgacccgttc ctgcagcagt cgcagcgcct cgctgccgaa tggccggccg
    16381 cagcggttca cgaggaagcc gggaccgtcg accgccacga tcacgtgttt gcccatcgct
    16441 tcgcccgtcg cgcgcgcgac ggcgagtgct cgctcgccgg tctgcgccgc ggcgatcacc
    16501 tcgagcaact tcatcagcgg cggcgggttg aagaagtgca tgcccacgac gttctcgggg
    16561 cgtgctgccg ccgtcgcgat cgagctgacg aggatcgacg acgtgttcgt cgcgagcacc
    16621 gtctcggaag agcagacctc cgagagccgc gcgaacagct cgcgtttgag ctcgggtcgc
    16681 tccggtgcag cctcgatcac cagctccgtc tcggaaaggt cgtcgacgga cggcgccgcg
    16741 tgcagccgct cctccgccgc gcgcgcctcg tcctcgctcc accgaccgcg ctcggcgccc
    16801 ctgcgcaggc cctcccgaag ccgctcgata ccgcgctcga gcgcgtcctc gaaggggtca
    16861 tgaagccgtg tctcgatccc ggcgagcacg ccgagctgag cgatccccgc gcccatcgtt
    16921 ccggcgccga ccacgccgag tcgctggggt gtcgaggagt cgggcggggg cacgcggggc
    16981 cgcatgataa gcggctcgtc gttagcatcc ggcgccgccg caggtcaccc gccccgccgg
    17041 tacgccgcgc tctcgcgggc gcacgggacg caaatccgcg gcacaccaga ggggcctgtc
    17101 cgccggctta cgtctttagg gcggcgcccc gcaacttgtt cccggccgag cctgtttgtc
    17161 agatcgatgc ccgaaaccaa gatgctcttc aacttgaacc gaatcggcgc gcggatcgcg
    17221 ctggcgggcc tgctgggcgc cgccgcgatc ggcgcgctcg cgtcgcccgc acacgcctcc
    17281 cgcacacagg agtccgtgat gcaggacgac cgcctgctca tcaagagcgg ctctgccgtg
    17341 cgcgagcagg cgctgaacga catgcaatcc cttggggtgg acaccgttca ctcgctggcc
    17401 gtctggcgcg acttcgcgcc gaacgcggac tcgcgcacga agccgagcgg cttcgacggc
    17461 agcgatcccg cggcttaccc cccgacggcc tgggacgcgc tcgacgatct cgtccgcacg
    17521 cttcacgcgc gcggtatgag cctcatcctc acgccgaccg gcttcacgcc gtcgtgggcg
    17581 ggcagctgcg gctccgcgca caatcgccag gtctgcggcc cgaactccgc ggagttcggc
    17641 aagttcgtcg ccgcgctcgg caagcgctac tcgggcacgt accacgacga gaaccagggc
    17701 ggcgcggtgc tccccgccgt cacgcgctgg tcgatctgga acgaggcaaa cctcggcgcc
    17761 tggctgcagc cccagttcgt cgtgcgcaag ggtcgggccg tgccgaaggc tccgcacatc
    17821 taccgcgctc tggcgaccgc ggcgatcggc tcgcttcagg ccaccggcca ctctggcgac
    17881 cagatcatgc tcggtgagct cgcacccctc gggcagaaga cgggcaggcc cgccagccgc
    17941 aacaccgctc cggtcacgtt ctggcaggag atgctgtgtc tcggctcgaa cggccggcct
    18001 ctgaccggga gcgcgcggaa ggcgcgcggc tgtcccgcga agttcccgcg cttccaggta
    18061 aacgccgtgt cgcaccaccc gtacaaccgc ggtggcgcca gttcgccgct cactcgcggg
    18121 aacccgggcg atatcacgct cgcgacgatc tcgcggctgc agagcgtgct gactcagggc
    18181 tctcgcgcaa ggcgaatccc gagcaacctg ccgatctact acacggagtt cggcttccag
    18241 acgaacccgc cggaccgcac gttcggcatg tcgctcagcc gccaggccga gtacatcaac
    18301 gagtccgact acatcgcgta ccgcaacagc cggattgcgt cggtcgcgca gtacgagctc
    18361 aacgacgagc cgaatctggc gagcttccag accgggcttc gcttcgaggc gggcaaggcc
    18421 aagccgagct acgccgccta ccgccttccg atctacgtgg tccggcacgg ctcgaaggtc
    18481 ggcgtgttcg gacaggtgcg cccggctccc gatggctcga gcgataccgt gctgatcgag
    18541 aaccgcagcg gtcgcacgtg gaatgtggtg aagacgatca aggtgagcag ccagaagggc
    18601 tacttcctcg tcaacaccaa gtaccgctcg gggagctggc gactgtcgtg gacgccatcc
    18661 ggcgggggct cgacggtgtt cagccgctcg gcggcggcgc gctcccgcta gggacgcgcc
    18721 gcctcagcgg ccgcggaact gcggcttgcg cttctcgatg aaggcagaca tgccctcgac
    18781 gcggtcctcg gtcgcgagcg ccagctcgaa cagccggcgc tcgtgggcga ggcccgcgcc
    18841 gagcggcgtc tcgtcggcgg cgagcacggc ctgcttgccg agcttcacgg cgatcggggc
    18901 gcggcgggcc accacctcgg ccagctcgac ggcatcttcg agccacttgc gcttggcggc
    18961 aagacggttc acgagccccc agtcgtaggc ctcctgcgcc gtgatgcgcc gcccggtgag
    19021 caccagctcc atcgcacgct gcttgccgat gacgcgcgtg agccgctggg tgccgccgcc
    19081 gcccgggatg atcccgagca tgatctcggg ctggccgaac tccgcggtct cggacgcgac
    19141 gatcatgtcg cagccgagcg cgagctcgca accgcccccg agcgcgtagc ccgacaccgc
    19201 ggcgatgagc ggcgtgcggc agccggcgag cttcggccag aactcgctgc tcggctgcgc
    19261 gagcgcgtcc tgccaggcgc ggtcgcgcat cgacttgatg tcggcgcccg cggcgaacca
    19321 ctcgtcggaa cccgcgatca cgatgcaacc gatcgcgtcg tcgcggtcgt agctctggat
    19381 cgcctcaccg agctcagcga acagctcggg ggagagggcg ttgcgcgcgt cgggccggtt
    19441 cagccgcgcg atgccgaccg ccccgctcgt ctcgacgatg acgaccggcg cctgcgccat
    19501 caggtcatcg cgttgatgcc ggtggcggca cgcccgatga tcagcttctg gatctgcgag
    19561 gtgccctcgt acagcgtggt cacgcgcgcg tcgcgcaggt agcgctcgac cggatagtcg
    19621 tccacgtacc cggaaccgcc gtgcacctgg atcgctgcgt tcgccgaccg caccgcggcc
    19681 tcggtcgcgt acagcttcgc aacggaggtc tcggtcgtgc tcggctttcc ggagtccttc
    19741 agccaggccg cgcgccagca cagcatgcgg gccgcctcgg tgtccacgac catgtcggcg
    19801 atcagctcct gcacgagctg gaagctcgcg atcggccgat cgaactgcgt gcgctccttc
    19861 gagtaggcca cggacgcgtc cacgcagccc tggcagatcc cgacgcagcc agacgcgacg
    19921 ctgaagcggc ccgagtcgag cgccgacatc gcgaccttga acccgtcgcc gatctcgccc
    19981 atcagcgcct cgtcgccgac ctcgacctcg tcgagcgata gctccgctgt gtcggaggcg
    20041 cgcaaaccga gcttcccgtg caccggactc gagctgaatc cgggcgagtc ggtcggcacc
    20101 aggaacgccg cgaggccgcg gtgcttctgc tcgggatcgg tctgggcgaa gatcagcgcg
    20161 agcttcgcct tcgtgccgtt cgagatccac tgcttctgcc cggagatctt ccagccgtcg
    20221 gccgtcttgg tggcgcgcgt cttgaggctc gccgcgtcgg agccggtgtc cggctcggtc
    20281 agcccgaagc agccgaggat ctcgccgctg caaagcttcg gcaaccactg gtgcttctgc
    20341 tcctcgctcc cccagcgcac gatcgccgag cacacgagcg aggtctgaac cgaaacgacc
    20401 gtccgcgccg aggagtcgcc gcggccgatc tgctcgacga tcaggccata gctcatgtag
    20461 tcgagcccgc gaccgccgta ttcctccggg acgatcgcgc cgaggaagcc catatccgcg
    20521 atcttcttca cgagctcctc gtcgaagcgc tcgttgcggt cgttgtcgcg ggcgaccgga
    20581 atgatctccc ggtcggtgaa gtcgcgcgcg gtctcgcgga tcaggcgctg ctcatcggtc
    20641 agctcgaggt cgatcacagg gcggccaatc tactcgtcgc gggccgccac cgacgcatcc
    20701 tcgtacgcgg cgcgcacggc gcgatccgtc tccttcacct ggtccggcac gatcgcgcgc
    20761 gccatcgcgt cgcgcaacct gatcggcagc gcggcgtata tgcgcgacac caccgagagg
    20821 ctcgccggca cgtagacctc gaagcgcggc ttgcgcacgg cggccgccac cgcgcggccg
    20881 acgtcctcgg gcgtgaggcg cttcgtgcgg ccgctgctcg tgcccttcgc aagctcggtc
    20941 tcgacgaccg cgggcatcac gatcgtgatg tcgatgccgg tcttctcgag ctccgcgcga
    21001 acggctgccg tgtagccgta gaccgcgtgc ttgctggcgg tgtaggtcgc ctcgccggca
    21061 ggcgtgacct tgctcgccat cgacgccacg ttcacgacgt ggccacggcc gcggcgaagc
    21121 atgcgaggaa tggcgagctt catgccgcgc atggtgccgt gcgtgttcac cgcgaactga
    21181 cgtgcctctg cgctgcccgg ctcgtcgctg aagggcccga cccacatgat ccccgcgttg
    21241 ttcacgagca cgtcgagcgg gccgagctcg ttctcgaccg cgtcgaggaa ggcactgaac
    21301 gactcggtgc tcgtcacgtc gagctcgaga ccgatggcgc cgcccccgat gcgctccgcg
    21361 gtctggcgcg cggcctcacc gtcgatgtcg ccaagcgcga cgcgcgcgcc gtcgcgggag
    21421 agcgcctctc cgatcgcggc gccgattccc cgggcgccgc cggtgatccc gaccacccgg
    21481 ccggtcaaga cgcccgctgc gtgatctgct ggagcgccca gcggttgccg tccgggtcgc
    21541 tgaagaacac gaaccggccc cagggaaact cctgcacctc gctcacctcg acgccgcgct
    21601 ccacgagctc ggcgcgggcg gcgtccgcgt ctgcaacgac cacctgcagg ccctcgatgg
    21661 agccgggctc catttgcgtg agccccgtac cgatcgcgat cgacgcgggc gagcccgggg
    21721 gcgtcagctg cacgaaccgc agtccctcct gcgggctcac atcgacgtcc gcgttgaacc
    21781 gcagcttctc ggtgtagaac gccttcgcgc ggtcgacgtc ggagacgggc acctgcacga
    21841 gctcgagccg ccagtccatg agcggcggca gtttatccgg cgagcgatga gtttccgccc
    21901 gacccccccg tctggtggcc gagcatgaat ttctgcaggc gaccgggccc ctgacgacaa
    21961 gcgcctgcag ttattcacgc catacgtgaa tttctgcagg cgaccgcaat tgaagccgcc
    22021 agttgcctgc agttgttcat gtccgcggcc cgggcgcgca gcattgcgcc gccaggccca
    22081 cctcacctca tggctttcag acggcttcga cgatcgtcgc caggccctgg cccacgccga
    22141 tgcacatcgt ggcgaggcct agcttcgcgc cgcggcgccg catctcgtgc acgagcgtgg
    22201 tcagcaggcg cgcgcccgag cacccgagtg gatgaccgag cgcgatggcg ccgccgttca
    22261 cgttcagctt ctcctgcggg atcccgagct cgcgggcgca ggcgagcgac tgcgaggcga
    22321 aggcctcgtt cagctcgacg aggtcgatgt cgtcgatcgt gagcccagcg cgctcgagtg
    22381 cctggcgcgt agcggggatc gggcccacgc ccatgtagcc ggggtcgacg ccggcgacgc
    22441 ccgtcgacac gatccgcgcg agcggctcgc gcccaagctg ctctgcccta tccgggctcg
    22501 cgagcacgag gcaggccgcg ccgtcgttga tctgcgacga gttgccggcg gtcacgctgc
    22561 cgccctcgcg gaacacggcc cgcagtcctg cgagccgctc cagcgtggtt tcgggacgcg
    22621 ggccctcgtc ggagtggacg gtcaccggcg gaccttttgg ctggggcgcg tcgacggtca
    22681 cgatctcgtc gtcgaagcgc cccgcctcgg cggccgccac agcgcgctcg tgcgactgga
    22741 gcgcgaaccg gtcctggtcc tcgcgcgaga tggagtagcg ctcggccacg ttctcggccg
    22801 tctcacccat cgactcggtc gagtagttct cgcgcatgcg cgggttcacg aagcgccagc
    22861 cgatcgtcgt gtccacgagc tgcatgtcgc cccgcggcag cgaccgttcg ggcttcagca
    22921 tcacgaacgg tgagcggctc attgactcca cgccgccggc gagcatcagg tcgccgtcgc
    22981 cgaccatgat cgcgcgcgcc gcctgattca ccgcctctag gccggacgcg cagagccggt
    23041 tcacggtgac gcccggcacc tccaccggaa gcccggccag cagtgccgac atccgcgcaa
    23101 cgttgcggtt gtcctcgccc gctgcgttcg tgcagccgag gtacacgtcg gcgatgtcgc
    23161 cgcccgggag gccggtgcgc tcgaccacag cttcgatcac acgcgctgcc agatcgtccg
    23221 ggcggacggt cgagagcgca cctccgtagc ggccgatcgg cgtccggacc gcatcgatga
    23281 tgagcgcgtc gggcacgcga tgatcctaga ctggcccggt ggagttcgcg ctcgccgcga
    23341 cgggcgtcat cgtcgtgctc gcgatcgttt tcatcttgat cgggcacttc tacccgggct
    23401 ccggcgccga cgtgctggac tggcgcccca cgcgctcctt cgacgaggag gcgcgactcg
    23461 agctcgagga catcgaccag atgctcgagg cgcagaacga gcgtcgccgc cgctcgggcc
    23521 ggccggagct cagcgaggcc ggcatccagg cggaggtcga ctcggagcgc cgcgcgatgc
    23581 tcgagcgcgg cgagcgctac cgcggaagcg aggaggggcc tacgccgcct ccacagtccg
    23641 acgggtaagc ggtttgtcgc aggcgccggc agtgtcggcg atccaagcgc gcagcagacg
    23701 gttgaagcgc cccggcatct cgatctgcgg gtagtggccg cagcgctcga ggatgtgcac
    23761 gtcggcgtct gggtgagcct gttggaacgc gagtccctcc gacacgggca cgagtccgtc
    23821 gtgccggccc cacaccatga acttcgggca ggtgatcgcc ttcgagtcct cgagcagcgt
    23881 gccggtgtag gacgcgatgc aggcgcggcc cgcctcgagc agggcgccga aatccttcgt
    23941 cgtggagtgg aggtccacca ggtgacggat gtcctggcga agcgcccgcg agtcgtggaa
    24001 caggcgcagg tcgaggaagc ggctcatccc ctcgcggatg atcagcggcg tgagagggct
    24061 cggaatcgcg agcgctgcac gccaggccac gccgttcccg gcgatcggcc gccaccacca
    24121 aggcggtttc ttgccgagcc ccgccggcgc gaccagaccg agccgtgcca cgcgcttggg
    24181 gttcgccagc gcgaagcgca gcgcgatcgc gccaccgagc gagtggccga ccagcgtcgc
    24241 ggacttgatc tcgagacggt cgagcagcgc ggggaagtac cccgcgtaga agtcgaacag
    24301 ctcgcgttcg ctgatacgcc cggatcggcc gaacggcggc acgtcgatcg cgatcacgcg
    24361 gttcgtctcg agcaaccctg gcacgacacg tcgccagccg tccgcggtgt cgccgtagcc
    24421 gtggacgaag aggatcggag atccctcgcc ggcgtcgacc gtgtaggtcg caacgccatc
    24481 cacgtcgagc agctggtgct ccagctcgcg gcccttcgcg tcgtgcaggc cggggtgacg
    24541 gggagtgcgg gtcatcgaag ctggtcctcg taacagttcg cttcgacgcc gcttggattt
    24601 gtccctgcca ttcgaaccgt ccctggtcca ggtgaagggg aagtcccact tccaacgtaa
    24661 ggccaaacgg gtgatccgga tagcctcccg cgcgtggcgc gcgtcctggt agtcggctgc
    24721 gggtgccgcg gtcttgagct ggctcgcgcg ctcgctgcgg acggacacgc ggtccggggc
    24781 accacgcgtc ggcaggaacg gctcccggag atcgaggccg cgggcgccca ggctgcgctc
    24841 gccgacccgg atcgcctggc cacgatgctg cctcacattg acggcgtggc ggtgatgtgc
    24901 tggatcatgg gaagcgcctc gggcgagcca gaccacgtgg cggcgctgca cgggccacga
    24961 ctcgagtcaa tggtcgagac attggtggac acgcacgtgc gtggggtcgt ctacgaggcg
    25021 gcggggagtg tggaccaggc gcttctcgaa cacggcgccg cgagcgtgcg gatggcgggc
    25081 gcgcgcttcc acatgcccgt cgaagtcgtg gacactgatc cccgcgaacg gacggattgg
    25141 ctcgaggcga tgcgcgcagc ggtggcgcgc gtactcgccg cctagattcg ctcgatcagg
    25201 ccggtcgcgc ccgcgaccgt gaggaagatc gccgcaagag cgagcaccac ggcgaccgcg
    25261 aacagcgcat gccagctcgt gtcgaccgcg atctcgcggc gggtgcgcgc cggcggctcg
    25321 agcggcccgg gcactgggcc gacgggcacc gcgatcaccg gcggcggcgg tggggcggag
    25381 ggcgcgggcg gctcgcccac gtcgggcggc tcggctgcga tcacttcgtc cggagcggcc
    25441 gcgacgacct
//
LOCUS       DASVXS010000306         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_902813, whole genome shotgun sequence.
ACCESSION   DASVXS010000306 DASVXS010000000
VERSION     DASVXS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902813"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..755)
                     /locus_tag="VF752_17235"
     CDS             complement(195..755)
                     /locus_tag="VF752_17235"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8857341.1"
                     /translation="MNADALSATPAAGAALEALRAAAGSDSPLERHCLRQFAIAERLA
                     GKRTFDRELLLCACWLHDLGLFTLSRDPYVTEGARLAARVLEPFSWPPERLQRCMDAC
                     EQHHAPRSRVAMGLEVELVRQADLVDVTAGLVNFGLDRGWLRGLFREVPRDGLWRLIG
                     SAVLNELRHRPASLSGVFLSPRRTSL"
     gene            complement(968..1360)
                     /locus_tag="VF752_17240"
     CDS             complement(968..1360)
                     /locus_tag="VF752_17240"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8857342.1"
                     /translation="MSQENVEIVRRAFAYEVYGVGDRAEAEAIFDPDVVMNPNNEGPY
                     HGLDAMREDFERWASAFEELKVSAEEFIDAGDRVLVTAYHRGRGRTSGVEVDGRFYEV
                     YTLRDGKVVRIDEYVDRAEALEAVGLRE"
     gene            1535..1933
                     /locus_tag="VF752_17245"
     CDS             1535..1933
                     /locus_tag="VF752_17245"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8857343.1"
                     /translation="MSRQNVEVVRAIYRAFARRDRDALVRHLDSSIRVYDRPLHPDAS
                     VYEGREGFLRFSETDWEAFEEVVYEPQDFLANGPYVVVPIKQSGRGKSSALDVEERIV
                     NVWKMRHGKCVELRIFSTIEEALEATGPPA"
     gene            complement(1930..2574)
                     /locus_tag="VF752_17250"
     CDS             complement(1930..2574)
                     /locus_tag="VF752_17250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8857344.1"
                     /translation="MPLDISARPIFARCLARSAAISERKGGDAYRRRLLAGLTGRVVE
                     VGAGSGINFHHYPRGVAEVLAVEPEANLRAMAAAASREAPVPVRVVPGLAEALPAPDA
                     SADAGVSAGLLCSVPDPAAALAELARVIRPGGELRFFEHVVSPRPRAASFQRGLDASG
                     VWASAMGGCHTSRETEAAIARAGFRIESIERFTFRPTLLDLPVAPKILGRARRS"
BASE COUNT          458 a          968 c          908 g          404 t
ORIGIN      
        1 aacatggtgt ctacgaggac gatcgcgcag gcgaggagga cgagacggcg cactctcgcg
       61 atgctacggg gccgggcgta tgaggcccgc tcgcagctcg cgaaggcggc acactgactt
      121 ctgctttcac gcgaaagcga aaggcaatcc atcgcgcgaa tcgcgtcgag cacaagccag
      181 caacgcggct ggggctagag agacgtgcgt cgcggggaga gaaagacccc gctgaggctc
      241 gccgggcgat ggcgcagctc gttgagcacc gccgagccga tcaggcgcca gagaccatct
      301 cgcgggacct cgcggaacag tccacgcagc cagccccgat cgaggccaaa gttcacgagc
      361 cctgcggtga cgtccacaag atccgcttgc cgcacgagct cgacctcgag gcccatcgcc
      421 acccgtgagc gcggcgcgtg gtgctgctcg caggcatcca tgcagcgctg caaccgctcg
      481 ggtggccagc tgaacggctc cagcacccgg gcggccaggc gtgcgccctc cgtcacgtac
      541 gggtcgcggc tgagggtgaa caggccgagg tcgtgcagcc agcacgcgca cagcagaagc
      601 tcgcgatcga acgtgcgctt gccggcgaga cgctcggcga tcgcgaactg ccgcagacag
      661 tggcgctcga gcggactgtc tgatcccgct gcagcacgga gcgcctccaa ggccgcgcca
      721 gcggccgggg tcgcggacag ggcgtcggcg ttcatgcggg cgagcctagc ggcgggcaac
      781 ggaacgacgc tccccggcgc gagataccgg tctccgccac cgctcagtct gcgctccccg
      841 cgcacgaagg cgtccgtcct gggtccacca tcgagctcgc agcgccgcct tacgcgagta
      901 ggggcagcgc gcttgacttc cggcggcgcg ggtgacttcc gctgctcgtc cattggggta
      961 gtccggatta ctcccgcagc cccacggctt cgagggcctc ggcccggtct acgtattcgt
     1021 cgatacggac caccttgccg tcgcgcaggg tatagacctc atagaagcgg ccatcgacct
     1081 cgaccccgct ggtccggcct cgcccccggt gataagccgt caccagcaca cggtccccgg
     1141 cgtcgatgaa ctcctcggcg cttaccttca gctcttcaaa ggcgctggcc cagcgctcga
     1201 agtcttcccg catcgcatcc aaaccgtggt atggcccctc attgttcggg ttcatcacga
     1261 cgtcgggatc aaagatcgct tctgcctcgg cgcggtcgcc gactccgtag acctcgtagg
     1321 cgaaagcccg ccgcacgatc tccacgttct cctgcgacat cgcccgccca gtatctcgcg
     1381 gcgcggttgc gccttccacg caatgggcgg tcgcacaccg actgccgctt ttgcgcgatt
     1441 tgcggcagcg ccggcctacg ccattcgaga cgcctgcctc tcgtcccctg gcctgcttgt
     1501 ccctcactca agacggcgcg atactcgcgc gacggtgtcc cgccagaacg tcgaggtcgt
     1561 gcgcgcgatc taccgcgcct ttgcccgccg cgaccgtgac gcgctggtgc gacatctcga
     1621 ttcttccatc cgggtctacg accgcccgct tcatccggac gcatccgtct acgagggccg
     1681 cgaggggttc ctgcgcttta gcgagaccga ctgggaagca tttgaagagg tcgtctacga
     1741 gccccaggat ttcctggcca acgggcccta cgtcgtggtc ccgatcaagc agagcgggag
     1801 aggcaagagc agcgcgctcg acgtcgagga gcgcatcgtc aacgtctgga aaatgcggca
     1861 cgggaagtgc gtcgaactgc ggatcttctc cacgatcgag gaggctctcg aagccacggg
     1921 accgcccgct tagcttctcc gtgcgcggcc gaggatcttc ggcgcaacgg ggaggtcgag
     1981 cagcgtggga cgaaacgtga agcgctcgat cgactcgatg cgaaacccgg cgcgcgcgat
     2041 cgcggcctcc gtctcgcggc tcgtgtgaca gccgcccatg gcgctcgccc agacaccgct
     2101 cgcgtcgagc ccgcgctgga aactcgcggc gcgtggtcga ggcgaaacga cgtgctcgaa
     2161 gaagcgcagc tcgccgcccg gtcggatcac gcgagcgagc tcggccagag cggcggccgg
     2221 gtcgggcacg ctgcagagaa ggccggccga gacgccggcg tccgccgacg cgtccggcgc
     2281 cggcaacgcc tcggccaggc cgggcaccac acgcacggga accggtgcct cccgtgacgc
     2341 agccgccgcc atcgcgcgca ggttcgcctc gggctcgacc gcgaggacct ccgcgacgcc
     2401 gcgcggatag tggtggaagt tgatcccgct gcccgcgccc acttccacca cgcgcccggt
     2461 taggccggcc agcagccggc gccgatacgc gtcgccaccc ttgcgctcgg aaattgcggc
     2521 cgagcgagct aggcagcgcg cgaagatggg ccgagcggag atgtcgagcg gcacgctcgt
     2581 cagcttttca aacgacgtcg cccgcttctg ctgacgcttg cgctccgcgg ggctagcctc
     2641 gtcctcaagt cacgggagag gagaggccat gaccttccga gtcggggatc gagtcgaagc
     2701 agagtccgag tcgaccgagc gcccagcgcg tatcggca
//
LOCUS       DASVXS010000307         6312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_1114284, whole genome shotgun sequence.
ACCESSION   DASVXS010000307 DASVXS010000000
VERSION     DASVXS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114284"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VF752_17255"
     CDS             complement(<1..404)
                     /locus_tag="VF752_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857345.1"
                     /translation="MRRLAPAVIAVVTAALLAAGCGGSGASTAPPPKGPPSARHHGKA
                     ANYLTDIERTRAGLVSAADLVYIMHPVGARNQVDAASKAYAPLSADVAAQIPALDHEI
                     RAAFGTNDELIARRAPFASLQVRLGLVQGQLL"
     gene            complement(401..823)
                     /locus_tag="VF752_17260"
     CDS             complement(401..823)
                     /locus_tag="VF752_17260"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX8857346.1"
                     /translation="MARERDYSHRTLAQKLGVKQDERVEVVGDVGPSVRRDVKDAIGR
                     GFVKSGELDGAIVLIESLEDAEEAFDRYRPRLRDTGYLWFVTRKRGHDNYLNQMLLVP
                     AAKRRGLIDNKTCSIDDDRSGIRFVVPRSLRAPKQTAA"
     gene            962..1810
                     /gene="dapA"
                     /locus_tag="VF752_17265"
     CDS             962..1810
                     /gene="dapA"
                     /locus_tag="VF752_17265"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX8857347.1"
                     /translation="MTFGGVLTAMVTPFDDRGRLAEDASARLVRHLLDHGSDGLVVAG
                     TTGEGPTLTDEEKLALWEICVAEAGGAPVIAGTGSNDTAHTVELTERAGDIGVDGMLV
                     VTPYYNKPNRRGVKAHFEAVAAATDKPIVVYNIPSRCVVDIPNDLLRELAEIENVAAV
                     KQARDTDVELIDGLDLLAGNDDMFARVLDMGGAGGILVASHIVGEEMRRMVDEPEQRA
                     QIDRSLEDVYAAMSVTTNPIPVKCALNMLGIDVGGLRLPMVDADESEAAAIRAALERH
                     GLLSAV"
     gene            1807..3477
                     /locus_tag="VF752_17270"
     CDS             1807..3477
                     /locus_tag="VF752_17270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX8857348.1"
                     /translation="MSGTLRVLPLGGLGEIGKNMTVLEYEGRLVVVDMGLMFPTPDQL
                     GIDLVLPDFTYLRERAADIDAVFLTHGHEDHVGALPFVLREIGEENTPDIYGGALTVA
                     MVRSKLDEHRLRDVPLYEVAPGEVVEAGPFSVEPVKMAHSIPDSFAYAVTCELGTTLV
                     TGDYKFDQTPVDGEPADVARLAELGREGLLLLCGDSTNVDRPGIALSESSVGPHLEEA
                     FARCDGRIIVTCFASNIHRVQQVVDAAQALGRKVSLIGRSMRKNTNIGQRLGHIHIPE
                     GMLVSAKEVEDFPDHKLVVISTGSQGEPLSALRRMAHGDHPNVSLHDGDTVIFSATPI
                     PGNERAVNETIDRIYQLGADVITPRDAPIHATGHGQAEELKLMINLTNPQYVLPVHGD
                     HKRIRMHAELAESLGVEPRNIFRGENGLPLEITELTARFAEPEQAGMIFVDGMEVGDV
                     SDVALRDRRMLSADGIFIVVATISEQDGRSVAPPEIIFRGVPFADDADDVLVEEIREA
                     VEQSLERSAEEEIREIDLLQSHLHDDVAKFVYDRMRRRPMVLPVVVEV"
     gene            complement(3474..4913)
                     /locus_tag="VF752_17275"
     CDS             complement(3474..4913)
                     /locus_tag="VF752_17275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8857349.1"
                     /translation="MKRLAALPVRWRLALTSAGLTFVILLLFAVVVGVFTANRLYGNF
                     DDDLRVAAADIQDRLTIYPLQGKINVRQSPDELDAAATGHAIIRVIDTRGGLIGDAPQ
                     NSPQLGPPDAGIRDAGKYRVVTRPLFIGGEDSPAAFLQYARPKSTVDSTVDRMHLFLA
                     LGVVGGTLLALLAGLAVAERAMQPVMQLTATAKKIARTRDPALQMPEPETDDEVADLA
                     RTLDQMLRALDSARSETEAMLERQREFVADASHELRTPLTSILTNLELLEAELNGDQR
                     EMADSALRSSRRMRVLVSDLLLLARADAGQRSPRKVTDLSRVVREAAAEVAPVAPDHE
                     LSVDAEDGALVDGSPDELHRLAVNLLHNATVHTPSGTHVRATLRREGRSVVLEVADDG
                     PGVPEELRDRVFERFVRAAGDSAVAARRGTGLGLAIVQAVAQGHGGTVELADSEVGAR
                     FVVTLPASTLPDTQDVPAEADAAPASERR"
     gene            complement(4910..5590)
                     /locus_tag="VF752_17280"
     CDS             complement(4910..5590)
                     /locus_tag="VF752_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8857350.1"
                     /translation="MATKPRVLVVEDDAEIGGALRRSLRLEGYDVRLAEDGVAALEES
                     SLFEPDAVVLDLGLPRLDGMDVAKRLRESSDVPILMLTARDATDARVAGLDAGADDYL
                     VKPFERDELLARLRALLRRRPPRGSAFLTVGDLRLNPDTREVFRGDRAVELTTREFEL
                     LEYLMRNERLVVSRDALLEKVWGYQVLVETNTVDVFVSNLRRKLESGGEPRMLHTVRG
                     AGYVLRAP"
     gene            5693..>6312
                     /locus_tag="VF752_17285"
     CDS             5693..>6312
                     /locus_tag="VF752_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX8857351.1"
                     /translation="MASRRQTKKPARGRSGRSRARGRRSSGFTLPRLPQFDQRDLDLV
                     GLGLVAAGVFFASVFYLGWNGGKVGEALADGFIYLFGGVAYLVPIGLFGAGAVLVLRD
                     LIPSVRPFKAGAACLVLGLMLGLAAGSFGIGPDHPVRQGYLHADFVRNHGGLVGDVMY
                     WTTHTLVQQFGTDLIFLFLMFAGVLLLTGASVAGVVKATSESLTTTT"
BASE COUNT          941 a         2190 c         2236 g          945 t
ORIGIN      
        1 tcgagcaact gtccctgcac gagccccagc cgcacctgca ggctcgcgaa cggcgcgcgg
       61 cgcgcgatca gctcgtcgtt cgtgccgaat gccgcgcgga tctcgtggtc gagtgcgggg
      121 atctgcgccg ccacgtcggc cgagagcggg gcgtaggcct tcgaagcggc gtccacctgg
      181 ttgcgcgccc ctaccggatg catgatgtag accagatcgg ccgccgacac gagccccgcc
      241 cgcgtgcgct cgatgtcggt gaggtagttc gccgccttcc cgtggtgccg ggccgacggc
      301 ggccccttcg gcggcggggc ggtgctggca ccggacccac cgcaccccgc cgcgaggagc
      361 gcggccgtca cgaccgcgat gactgcggga gcgaggcgcc tcaagccgcg gtctgcttcg
      421 gcgcgcgcag gctgcgcggc accacgaagc gtatgccgga gcggtcgtcg tcgatcgagc
      481 aggtcttgtt gtcgatgagc ccgcgccgct tggcggccgg cacgagcagc atctggttga
      541 ggtagttgtc gtggccgcgc tttcgggtga cgaaccacaa gtagcccgtg tcgcgcaatc
      601 taggccggta gcggtcgaac gcctcctcgg cgtcctcgag cgactcgatg agcacgatcg
      661 cgccgtcgag ctcgcccgac ttcacgaacc cgcgcccgat cgcgtccttc acgtcgcggc
      721 gcacgctggg gccgacgtcg ccgaccacct cgacgcgctc gtcctgcttc acgcccagct
      781 tctgggcgag tgttctatgg ctgtaatcgc gctctctggc cacggaaacg gcttggaatt
      841 atgccctgtg caggggacgt tccgcgcggg tggatatgct cgcgcgaccc gccttaggag
      901 agacttaatg ggcagaatgg gcagattcat ccgccgagac cgcgggtggg ataatcgcgc
      961 cgtgactttc ggtggagtgc tcaccgcgat ggtcacgccg ttcgacgacc gcggccgcct
     1021 ggccgaggac gcatcggcgc ggctcgtccg gcatttgctg gaccacggca gcgacggcct
     1081 cgtggtggcc ggcaccaccg gcgagggacc gacgctaacg gacgaggaga agctggccct
     1141 ttgggagatc tgcgtggcgg aggccggcgg cgcgcccgtg atcgcgggaa ccggctcgaa
     1201 cgacacggcc cacacggtcg agctcactga gcgggccggc gacatcggcg tggacgggat
     1261 gctggtggtc acgccctact acaacaagcc gaaccggcgc ggggtgaagg cccatttcga
     1321 ggccgtggcg gcggccacgg acaagccgat cgtggtctac aacatcccgt cgcgctgcgt
     1381 ggtggacatc ccgaacgacc tgctccgcga acttgccgag atcgagaacg tggcggccgt
     1441 caagcaggcg cgggacacgg acgtcgagct gatcgacggg ctcgacctgc tcgcgggaaa
     1501 cgacgacatg ttcgcgcggg tgctggacat gggcggagcc ggcggcattc tcgtcgcctc
     1561 ccacatcgtc ggcgaggaga tgcggcggat ggtcgacgag cccgagcagc gcgcccagat
     1621 cgaccgctcg ctcgaggacg tgtacgcggc aatgtcggtc accacgaacc cgatccccgt
     1681 gaagtgcgcg ctgaacatgc tcgggatcga cgtgggcggg ctgcgcctgc ccatggtcga
     1741 cgctgacgag agcgaggcgg cggcgattcg cgcggcgctc gaacggcacg gactcctcag
     1801 cgcggtttga gcggcacgct gcgcgtcctg ccgctcggcg gcttgggcga gatcggcaag
     1861 aacatgaccg tcctcgagta cgagggacgc ctcgtggtcg tggacatggg cctgatgttt
     1921 cccacgcccg accaactcgg gatcgacctc gtgctgccgg acttcacgta tctccgcgag
     1981 cgcgcggcgg acatcgacgc cgtctttctc acccacgggc acgaggacca cgtgggcgcg
     2041 ctgcccttcg tgctgcggga gatcggcgag gagaacacgc ccgacatcta cggcggggcg
     2101 ctcaccgttg cgatggtgcg ctcgaagctc gacgagcacc ggctgcgcga cgtgccgctc
     2161 tacgaggtgg ctccaggcga ggtggtcgag gccggcccgt tcagcgtcga gccggtgaag
     2221 atggcccact cgatccccga ctcgttcgcg tacgcggtca cctgcgagct aggcacgacg
     2281 ctcgtcacgg gcgactacaa gttcgaccag acgccggtcg acggcgagcc cgccgacgtg
     2341 gcgcggctgg cggagctcgg ccgcgagggg ctgctcctgc tttgcggcga ttccacgaac
     2401 gtcgaccgcc cgggcatcgc gctgtccgag tcgagcgtgg gcccgcatct cgaggaggcg
     2461 ttcgcgcgct gcgacggccg catcatcgtc acctgcttcg cgtcgaacat ccaccgggtg
     2521 cagcaggtgg tggacgcggc acaggcactc gggcgaaagg tgtcgttgat cggccgctcg
     2581 atgcgcaaga acaccaacat cggccagcgg ctcggccaca tccacatccc ggaggggatg
     2641 ctggtgtccg ccaaagaggt ggaggacttc cccgaccaca agctggtcgt gatctccacc
     2701 ggcagccagg gcgagccgct gtcggcgctt cggcgcatgg cccacggcga tcacccgaac
     2761 gtgtcgctcc acgacggcga caccgtgatc ttctcggcca cgccgatccc cggcaacgag
     2821 cgcgcggtga acgagaccat cgaccgcatc taccagctcg gcgcagacgt gatcacgccg
     2881 cgcgacgctc ccatccacgc caccgggcac ggccaggcgg aggagctcaa gctgatgatc
     2941 aacctcacga atccgcagta cgtgctgccc gtgcacggcg accacaagcg catccgcatg
     3001 cacgcggagc tggcggagtc gctcggggtc gagccgcgca acatcttccg cggcgagaac
     3061 gggctgccgc tcgagatcac ggagctcacc gcgcgcttcg ccgagcctga gcaggcgggg
     3121 atgatcttcg tagacggcat ggaggtcggg gacgtctccg acgtcgcgct gcgcgatcgc
     3181 cgcatgctgt cggcggacgg gatcttcatc gtggttgcca cgatctccga gcaggacggg
     3241 cgctcggtcg ccccgcccga gatcatcttc cgcggcgtac cgttcgccga cgacgccgac
     3301 gacgtcctcg tggaggagat ccgcgaggcg gtggagcagt cgctagagcg ctccgccgag
     3361 gaggagatcc gggagatcga tctgctccag tcgcacctgc acgacgacgt ggcgaagttc
     3421 gtatacgacc gcatgcgccg gcggccgatg gtgctgccgg tcgtggtgga ggtctagcgc
     3481 cgctcgcttg cgggcgccgc gtcggcctcg gccggcacgt cctgcgtatc gggcagcgtc
     3541 gaggcgggca gcgtgaccac gaagcgcgct cccacctcgg aatccgcgag ctcgaccgtg
     3601 cccccgtggc cctgagcgac cgcttgcacg atcgcgagcc ctaggccggt cccgcgccgc
     3661 gcggccaccg cgctgtcgcc ggccgcgcgc acgaagcgct cgaatacgcg gtcgcgcagc
     3721 tcctccggca cgccggggcc gtcgtcggcg acctcgagca cgaccgagcg gccttcgcgc
     3781 cgcagggtcg cccgcacgtg cgtgccggac ggcgtgtgca cagtggcgtt gtggagcagg
     3841 ttcaccgcca ggcggtggag ctcgtcggga gagccgtcca cgagcgcgcc gtcctcggcg
     3901 tccaccgaga gctcgtggtc gggcgccacc ggcgccacct cggccgccgc ctcgcggact
     3961 acccgcgaga gatcggtgac cttgcgcggc gagcgctgcc ccgcgtcggc gcgggcgagc
     4021 aacagcaggt ccgacacgag cacgcgcatg cgccgcgacg agcgcaacgc ggagtcggcc
     4081 atctcgcgct ggtcgccgtt cagctcggcc tcgagcagct cgaggttcgt gaggatgctc
     4141 gtgagcgggg tgcggagctc atgcgaggcg tcggccacga actcgcgctg ccgttcgagc
     4201 atcgcctcgg tctcggagcg ggcggagtcg agcgcccgga gcatctggtc gagcgtgcgc
     4261 gccaggtcgg cgacctcgtc gtcggtctcg ggctcgggca tctgcagggc ggggtcgcgc
     4321 gtgcgcgcga tcttcttcgc cgtcgccgtc agctgcatga ccggctgcat cgcgcgctcg
     4381 gccaccgcga gccccgccag gagcgcaagc agcgtgccgc ccacgacgcc gagggcgagg
     4441 aagagatgca tgcggtccac ggtgctgtcg accgtgctct tcggccgtgc gtactgaagg
     4501 aaggccgcgg gggagtcctc gccgccgatg aacagcggcc tcgtgaccac gcggtacttg
     4561 cctgcgtcgc ggattcccgc gtccggcggg ccgagctgag ggctgttctg gggcgcatcg
     4621 ccgatcagcc cgccgcgggt gtcgatcacg cggatgatcg cgtggccggt cgcggcggcg
     4681 tcgagctcgt cgggcgactg gcgcacgttg atcttgccct gaagcggata gatggtgagc
     4741 cggtcctgga tgtccgccgc ggccacccgc aggtcgtcgt cgaagttgcc gtacagccgg
     4801 ttcgcggtga acacgccgac caccacggcg aacagcagca ggatcacgaa ggtgagcccg
     4861 gcggaggtga gcgcgagccg ccacctcacc gggagggcgg cgagccgctt catggcgcgc
     4921 gaagcacgta ccccgcgccg cgcacggtgt gcagcatgcg gggctcgccg cccgactcga
     4981 gcttgcggcg caggttggac acgaacacat caacggtatt cgtctcgacc agcacctggt
     5041 agccccacac cttctcgagc agggcgtcgc gcgagaccac gagccgctcg ttccgcatga
     5101 ggtactcgag cagctcgaac tcacgcgtgg tgagctccac cgcgcggtcg ccgcggaaca
     5161 cctcgcgagt gtccgggttg agccgcaggt cgcccaccgt gaggaacgcg ctgccgcgcg
     5221 gcggccggcg ccgaagcagg gcgcgcaggc gcgcgagcag ctcgtcgcgc tcgaacggct
     5281 tcacgagata gtcgtcggcg ccggcatcga gccccgccac gcgggcgtcg gtggcgtcgc
     5341 gcgcggtgag catcaggatt ggcacgtcgc tcgactcgcg caggcgcttc gccacgtcca
     5401 tgccgtcgag gcgcggcagc ccgaggtcga gcaccaccgc gtccggctcg aacagcgatg
     5461 actcctcgag agctgccaca ccgtcctcgg cgaggcgcac gtcgtagccc tcgagtcgca
     5521 gcgatcggcg aagcgcgccg ccgatttcag cgtcgtcctc gaccaccaaa acgcggggct
     5581 tcgtcgccat ctttagaaat ggtaggagcg ggccagccga tcgtgggcct cttttaagcg
     5641 ccctttagcg gcagggtcgc gcccggcggt gtcgaagagt cctgcgctga ggatggcaag
     5701 ccgccgccag accaagaaac ccgcaagggg acgatcaggc cgctcgcgcg cgcggggacg
     5761 gcgttcgtcc ggcttcacgc ttccgcggct gcctcagttc gaccagcgcg acctcgatct
     5821 cgtcgggctc gggctcgtcg cggccggcgt cttcttcgca agcgtcttct acctcggctg
     5881 gaacggcggc aaggtggggg aggcgctcgc cgacggtttc atctacctgt tcggcggcgt
     5941 cgcgtacctc gtcccgatcg gcctgttcgg cgccggcgcc gtgctcgtcc tccgcgacct
     6001 gatcccgtcg gtgcgcccgt tcaaggcggg cgccgcctgc ctggtgctgg gcctcatgct
     6061 cgggttggcg gccggctcct tcggcatcgg acccgaccat ccggtccggc agggctacct
     6121 gcatgcggac ttcgtccgta atcacggcgg actcgtgggc gacgtcatgt actggaccac
     6181 gcacacgctc gtgcagcagt tcggcaccga cctgatcttc ctgttcctga tgttcgccgg
     6241 cgtgctcctg ctcaccggcg cctccgtggc cggcgtggtc aaggccacga gcgaaagcct
     6301 cacgaccacc ac
//
LOCUS       DASVXS010000308         6458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_909704, whole genome shotgun sequence.
ACCESSION   DASVXS010000308 DASVXS010000000
VERSION     DASVXS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909704"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF752_17290"
     CDS             <1..665
                     /locus_tag="VF752_17290"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX8857352.1"
                     /translation="VELIYDLCMGIVILTFAFSQGVFPKPPDFSKLPAFDLAFFAQHP
                     QFTLFVLTALAVAVMIGIALTSERIRAFWSHVRQGFAILKDRERYLRQVFGVQFVGWL
                     FRFTAFWFMLDAFRIGGSVKNVLLVLGVQAVSAVVPFTPGGAGVQQALLVKVFAGTAA
                     SATVAAYSVGQQIAIAVFTFAMGFAALALIFRIRSFKEIIALGQAERAAEKQARAAQP
                     AG"
     gene            complement(662..943)
                     /locus_tag="VF752_17295"
     CDS             complement(662..943)
                     /locus_tag="VF752_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857353.1"
                     /translation="MSSEDPFGGLFGDPDELRRRMAEFAEQMQGAQRVAWADNAIQLA
                     VNMTVAGINRLNLQGNVDQQAEDVRRAMGILFPEAVTLVREAREGFGTS"
     gene            999..1445
                     /locus_tag="VF752_17300"
     CDS             999..1445
                     /locus_tag="VF752_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8857354.1"
                     /translation="MRISEVMTHSVVTADCAAPLSEVGALMRDRNVGSVVICEHGHPV
                     GVITDRDLALAVVADGVDSESSVGGHATRPLVTGEAQMELEEAVALMVQHHIRRLPVL
                     DGHELAGIVTIDDLAVRAGDLELAQHITAEVAKAALPEFFFHQRGG"
     gene            1447..1656
                     /locus_tag="VF752_17305"
     CDS             1447..1656
                     /locus_tag="VF752_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857355.1"
                     /translation="MSVLTAEQRRRRELVEGGIRLAAPVLNLVLAAGERVSRIVEPDD
                     PEYYPPRAGDIEPPPAPERKPRVSE"
     gene            1661..2686
                     /locus_tag="VF752_17310"
     CDS             1661..2686
                     /locus_tag="VF752_17310"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX8857356.1"
                     /translation="MTAAPPAPEDGATHSHREQLEWEKRTGKLVGAAAFVSLLLLVAS
                     MILYQRADIPGGSNNSAAALRAIDHHGSTVIATGVLQSLGIFLLVPILWFLYRVTKYR
                     RPQLPRIALVLAVTAPIVVGGVTVASQAEVVNAAGTFVKQKAPNASQAADQLSKLRTA
                     KQQEDAANKLDPNERAKDTLDTGTLRALSGLGLAANLALAFGFIMISLNAMRAGVVSR
                     FIGIVGIIIGVLYVVPLGGPQILQFFWLGAMGLLFLGRWPGAGRGPAWETGEAIPWPS
                     AADRHAETRGEAPPAARPPTLFGGRGRAPAPEPEQPAELPEVVAEAEEGETAGQRRKR
                     RKKKKRR"
     gene            complement(2725..3099)
                     /locus_tag="VF752_17315"
     CDS             complement(2725..3099)
                     /locus_tag="VF752_17315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8857357.1"
                     /translation="MATVQESLQVKSSSEPDEVRPYVDKGHMDVVHLSSGDAGLGTFE
                     PGWRWSECVKPIAGSDSCQVRHVGYMLSGRMVVTMDDGTEREAGPGDFVHIPPGHDAY
                     VVGDETCRFIDFGGLAGYAQAH"
     gene            complement(3169..4533)
                     /locus_tag="VF752_17320"
     CDS             complement(3169..4533)
                     /locus_tag="VF752_17320"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX8857358.1"
                     /translation="MSDETVKYTLSEREIPTHWVNLMPDLPGEPIPPLNPQTMEPAGP
                     PDLTPIFPEALIMQEVSPEPEVEIPEEVREVYKLWRPSPLYRARRLEKALDTPAHIYY
                     KYEGVSPAGSHKPNSAVAQAYENKQAGIKRLATETGAGQWGSALALACRLFDLECVIY
                     MVGSSYDQKPYRRSMMQTWGATVLRSPSETTDAGRSQLSHPTGSLGIAISEAVEVAAK
                     DPETNYSLGSVLNHVCLHQTVIGQEAMAQMALAGEEPDVVVGCVGGGSNFAGLTFPFI
                     RRVLRDGAKTRFLAAEPTACPTLTRGVYRYDFGDTVGMTPLLPMYTLGHDFVPPPVHA
                     GGLRYHGDAPILCALVKEGLIEARAYAQNPAFEAALMFARDEGIVPGPEPAHAIRATI
                     EEALAAREAGEERVILLGLSGHGHFDMSAYDDYLAGRLEDIEFSEADMQAALDRLPEA
                     PAIA"
     gene            4615..5397
                     /locus_tag="VF752_17325"
     CDS             4615..5397
                     /locus_tag="VF752_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857359.1"
                     /translation="MAREQPISPERKAAARDSSNAWERFNVQSGGLFAAAGMFLIDVA
                     PPGTATAIAAVGTVVTVRARYLEVTSRQLSDDPARTDFDVPVEPIRPPLHEGALGDDA
                     YAVAGMELARVAGDSGAWLSAVVLATERAEGASLAGKPDYEAQRLDEAHRFLLDASRA
                     LRDVERAANEFAAVLSGDAALRAAAGAPGARSAFDEVAERPLLEVMQPDVFALVLDTG
                     VERSELDFRIDRPDEADPVGDVASRLTEAGAAAAELGGQLGG"
     gene            complement(5449..>6458)
                     /locus_tag="VF752_17330"
     CDS             complement(5449..>6458)
                     /locus_tag="VF752_17330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8857360.1"
                     /translation="IVYGPLANGTTSVMYEGAPDYPDKDRWWEIVEQYGVTILYTAPT
                     AIRACIKWGDEYPGKHDLSSLRLLGTVGEPINPKAWIWYHKVIGGERCPIVDTWWQTE
                     TGHIMITPLPGITETKPGSATRPFPGIEAAVVNKDGEIVEEGGGYLTLRRPWPGMART
                     LYKEPERYVETYWSKWGPDVYVVGDAARVDEDGYFWIVGRTDDVINVSGHRLSTMEVE
                     SAIVSHERVAEAACIGATDEDTGQAIVAFVTLEGGDGWPDGFEDEIRAHVADRIGKLA
                     RPSRIIPADDLPKTRSGKIMRRLLKDIAEGRELGDVTTLRDPTVTEAIKQKIESGEVE
                     D"
BASE COUNT          912 a         2252 c         2233 g         1061 t
ORIGIN      
        1 tcgtcgagct gatctacgac ctgtgcatgg ggatcgtgat cctcacgttc gccttctcgc
       61 agggcgtttt cccgaagccg ccggacttct cgaagctgcc ggccttcgac ctcgccttct
      121 tcgcgcagca cccccagttc acgctgttcg tgctcaccgc gctcgccgtg gcggtgatga
      181 tcggcatcgc gctcacctcg gaacggattc gggcgttctg gtcgcacgtg cggcagggct
      241 tcgcgatcct caaggaccgc gagcggtacc tgcgccaggt gttcggcgtg cagttcgtcg
      301 gctggctctt ccgattcacc gccttctggt tcatgctcga cgcgttccgc atcggcggca
      361 gcgtgaagaa cgtgctgctc gtgctgggcg tgcaggcggt ctcggcggtg gtgccgttca
      421 cgcccggcgg cgccggcgtt cagcaggcgc ttctcgtgaa ggtgttcgca ggcaccgcgg
      481 cctcggccac ggttgcggcc tactccgtgg gccagcagat cgccattgcg gtcttcacgt
      541 tcgccatggg cttcgcggcg cttgcgctga tcttccggat ccgcagcttc aaggagatca
      601 tcgctctcgg gcaggccgag cgggccgctg aaaagcaggc gcgggcggcg cagccggccg
      661 gctagctggt tccgaagccc tcgcgcgcct cgcgcacgag cgtgacggcc tcggggaaga
      721 ggatccccat ggcccgccgg acatcctcgg cctgctgatc cacgttgccc tggagattca
      781 gccggttgat tcccgcgacc gtcatgttca cggccagctg gatggcgttg tccgcccagg
      841 cgacgcgttg agcaccctgc atctgctccg cgaattccgc catgcggcgc cgcagctcgt
      901 ccgggtcgcc gaagaggccg ccgaatggat cctctgagga catggagccc attctaggga
      961 gccgcaacat cgtgcattga gcacgtagac tcgcgttggt gcggattagc gaggtgatga
     1021 cgcattccgt ggtgacggcc gactgcgccg cgccgctcag cgaggtcggc gcgctcatgc
     1081 gtgaccggaa cgtcggctcg gtcgtgatct gcgagcacgg tcacccggtg ggcgtgatca
     1141 ccgaccgcga cctggcgctc gccgtggtgg cggacggggt cgactccgag tcctcggtgg
     1201 gcggccacgc cacgcgccct ctcgtcacgg gagaggccca gatggagctg gaggaggccg
     1261 tcgcgctgat ggtgcagcac cacatcaggc ggttgccggt gctcgacggc catgagctcg
     1321 ccggaatagt cacgatcgac gacctggccg tccgggcagg tgacctcgag cttgcccagc
     1381 acatcaccgc cgaggtggcg aaggccgccc tgcccgagtt cttcttccac cagaggggtg
     1441 gctgaggtga gcgtgctgac ggcggagcag cgtcgccggc gcgagctcgt ggaaggcggc
     1501 atccgcctcg cggcgcccgt gctcaatctc gtcctggcag cgggagagcg cgtgtcgcga
     1561 atagttgagc ccgacgatcc cgagtactac ccgccccgcg cgggggacat cgagcccccg
     1621 ccggcgcctg agaggaagcc aagagtcagt gagtagtgag atgaccgccg cgccgcctgc
     1681 ccccgaggac ggcgcgacac attcccatcg cgaacagctc gagtgggaga agcgcaccgg
     1741 caagctcgtg ggagcagccg cattcgtgtc gctgctcttg ctggtcgcca gcatgatcct
     1801 gtaccagcgg gccgacatcc ccggcggctc caacaattcc gccgcggctc tccgcgccat
     1861 cgaccaccac ggcagcacgg tgatcgcgac cggcgtgctc cagtcgctcg ggatcttcct
     1921 gctcgtgccg atcctctggt tcctttaccg ggtcacgaag taccgccgcc cgcagctgcc
     1981 gcgcatcgcg ctcgtgctcg ccgtcactgc accgatcgtc gtgggcggcg tgacggtcgc
     2041 gagccaggct gaggtggtga acgccgccgg gacgttcgtg aagcagaagg cacccaacgc
     2101 gagccaggct gccgatcagc tgagcaagct ccggaccgcc aagcagcagg aggacgcggc
     2161 gaacaagctc gatccgaacg agcgggcgaa ggacacgctc gacaccggaa cgcttcgcgc
     2221 gctaagcggc cttggcttgg ccgcgaacct ggcgctcgca ttcggcttca tcatgatctc
     2281 gctgaacgcg atgagggcgg gcgtggtgag ccgcttcatc gggatcgtcg ggatcatcat
     2341 cggcgtcctc tacgtggtcc cgctcggcgg gccgcaaatc ctgcagttct tctggctcgg
     2401 cgccatgggc ctgctgttcc tcgggcgctg gcccggtgcc ggacggggcc cggcctggga
     2461 gaccggcgag gcgatcccgt ggccgagcgc cgccgacagg cacgccgaga cccgtggcga
     2521 agcgccgccc gccgcgcggc cgccgaccct gttcgggggc cggggccgcg cgcccgcgcc
     2581 ggagcccgag cagccggccg agctgcccga ggtggtcgcc gaggccgagg agggtgaaac
     2641 cgccggccag cgccgcaagc ggcgcaagaa gaagaagcgc cgctagccgc taccggcggg
     2701 cgaggacgcc ggggcctgtc gtcactagtg ggcctgtgcg taccctgcga gcccgccgaa
     2761 gtcgatgaac cggcatgtct cgtcgccaac gacgtatgcg tcgtgacccg gcggaatgtg
     2821 gacgaagtcc ccggggccgg cttcgcgctc tgtcccgtcg tccatcgtca ccaccatgcg
     2881 gccggacagc atgtagccca cgtgccggac ctggcacgag tcgctgccgg cgatcggctt
     2941 cacgcattcc gaccaacgcc atcctggttc gaatgtcccg aggcctgcgt ctccgctcga
     3001 caagtggacg acgtccatat ggcctttgtc cacgtacggc cggacctcgt cgggctcgct
     3061 cgagctcttg acctgcaagc tctcctgaac ggttgccatt tgggctcctt tcggtgactc
     3121 ggcgcagacc gaccctacgc ctgccacgaa gccggatgca acccgccgct acgcgatcgc
     3181 gggcgcctcg ggcagccggt cgagcgcggc ctgcatgtcg gcctcggaga actcgatgtc
     3241 ctcgagccgg ccggcgaggt agtcgtcgta ggcggacatg tcgaagtgcc cgtgacctga
     3301 gaggcccagc aggatcacgc gctcctcgcc ggcctcgcgc gcggcgagcg cctcctcgat
     3361 ggtggcgcgg atcgcgtgcg cgggttcggg ccccggcacg attccctcgt cgcgcgcgaa
     3421 catgagcgcc gcctcgaacg ccgggttctg cgcgtaggcg cgagcctcga tcagcccctc
     3481 cttcacgagc gcgcacagaa tcggggcgtc gccgtggtag cgcaggccgc cggcgtgcac
     3541 gggcggcggc acgaagtcgt gcccgagcgt gtacatcggt agcagcggcg tcatgcccac
     3601 cgtgtcgccg aagtcgtagc ggtatacgcc gcgcgtcagc gtcgggcagg cggtcggctc
     3661 ggccgccaga aagcgggtct tcgcgccatc gcgcagcacg cgccggatga atgggaaggt
     3721 gagccctgcg aagttcgagc cgccgcccac gcatcccacg accacgtcgg gctcctcgcc
     3781 ggccagcgcc atctgcgcca tcgcctcctg gccgatcacc gtctggtgca ggcacacgtg
     3841 gttcagcacc gagccgagcg agtaattggt ctcggggtcc ttggcggcga cctcgaccgc
     3901 ctccgagatc gcgatcccca gcgagccggt cgggtgcgag agctgcgagc gcccggcgtc
     3961 agtcgtctcg ctcggcgagc ggagcacggt cgccccccac gtctgcatca tcgagcgccg
     4021 gtacggcttc tggtcgtagc tcgagccgac catgtagatc acgcactcga gatcgaacag
     4081 gcggcatgcg agcgccaggg ccgagcccca ctggcccgcg ccggtctccg tggcgaggcg
     4141 cttgatcccg gcctgcttgt tctcgtacgc ctgcgccacg gcgctgttcg gcttgtgcga
     4201 gcccgccggc gagacgccct cgtacttgta gtagatgtgc gccggcgtat cgagcgcctt
     4261 ctcgagccgc cgcgcgcggt agaggggcga cggtcgccag agcttgtaga cctctcgcac
     4321 ctcctccggg atctcgacct ccggctccgg cgagacctcc tgcatgatca gcgcctcggg
     4381 gaagatcggc gtgaggtccg gcggtcctgc gggctccatc gtctgcgggt tgagcggcgg
     4441 gatcggttcg cccggcaggt ccggcatgag attcacccag tgcgtcggaa tctctcgctc
     4501 cgacagcgtg tacttgacgg tctcgtcgct cactcaccgg ctccttggtc gctggatgcg
     4561 cgattgtaag cgggtgccgt ggcgaccggc gagcggggtg ggatagcgtt agtcatggcc
     4621 cgcgagcagc cgatcagccc tgagcgcaag gccgccgcgc gggacagctc gaacgcgtgg
     4681 gagcgtttca acgtccagtc gggtgggttg ttcgccgcgg ccggcatgtt tctgattgac
     4741 gtcgccccgc ccggcacagc gacggcgatc gccgccgtcg gcacggtcgt cactgtgcgc
     4801 gccaggtacc tcgaggtcac gtcacggcag ctctccgacg atccggcccg gaccgatttc
     4861 gacgtgcccg tggagccgat ccggccaccg cttcatgagg gtgccctcgg cgacgacgcg
     4921 tacgctgtgg cgggcatgga gctggcacgc gtcgccggcg actccggggc ttggctgagc
     4981 gcggtcgtac tcgcgaccga gcgggcggag ggcgcgagcc tcgccggcaa gcccgactac
     5041 gaggcgcagc ggctcgacga ggcgcaccgc ttcctgctcg acgcctcccg cgcactccgc
     5101 gacgttgagc gggcggctaa cgagttcgcc gctgtgctgt ccggggacgc cgcgctgcga
     5161 gcggctgccg gcgcgcccgg ggcgcggtct gcgttcgacg aggtggccga gcgccccctg
     5221 ctcgaggtca tgcaacccga cgtgttcgca ctggtcctcg atacgggcgt cgagaggtca
     5281 gagctcgact tcaggatcga tcgccctgac gaggccgatc cggtcggcga cgtcgcctca
     5341 cggctgaccg aagcgggagc ggctgcggcg gagctcggcg ggcagctcgg gggatgaggc
     5401 gaagcgggag gcgtgcccgc tagcgggcac gcctccgcgc ctcacgcctc agtcctcgac
     5461 ttcgccggac tcgatcttct gcttgatcgc ctcggtcacg gttgggtcgc gcagcgtggt
     5521 cacgtcgccg agctcgcggc cttccgcgat gtccttgagc aggcggcgca tgatcttgcc
     5581 ggagcgcgtc ttcggcaggt cgtcggccgg aatgatgcgc gacggccgcg ccagcttgcc
     5641 gatccggtcg gcgacgtgcg ctcgaatctc atcctcgaac ccgtcaggcc agccgtcgcc
     5701 gccctcgagg gtcacgaacg caacgatcgc ctgcccggtg tcctcgtcgg tcgcgccgat
     5761 gcaagccgcc tccgcgaccc gctcgtgcga gacgatcgcc gactcgacct ccatcgtcga
     5821 caagcggtgc cccgacacgt tgatcacgtc gtcggtgcgt ccgacgatcc agaagtaacc
     5881 gtcctcgtcg acgcgggcgg cgtcaccgac cacgtaaacg tccgggcccc acttcgacca
     5941 gtacgtctcg acgtagcgct cgggctcctt gtagagggtg cgcgccatgc ccggccatgg
     6001 ccggcgtagc gtgaggtagc cgccgccctc ctcgacgatc tcgccgtcct tgttcacgac
     6061 ggccgcctcg atgccgggga acggcctcgt ggccgaaccg ggcttcgtct cggtgatgcc
     6121 cggcagcggc gtgatcatga tgtggccggt ctccgtctgc caccacgtgt cgacgatcgg
     6181 gcagcgctcg ccgccgatca ccttgtggta ccagatccac gcctttggat tgatcggctc
     6241 gccgaccgtg ccgagcaggc gcagcgacga gaggtcgtgc ttgcccgggt actcgtcgcc
     6301 ccacttgatg cacgcgcgga tcgcggtcgg cgccgtgtag aggatcgtca cgccgtactg
     6361 ctcgacgatc tcccaccagc ggtccttgtc cgggtagtcg ggcgcgccct cgtacatcac
     6421 ggacgtcgtc ccgttggcga gcgggccgta gacgatgt
//
LOCUS       DASVXS010000309         6732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_126880, whole genome shotgun sequence.
ACCESSION   DASVXS010000309 DASVXS010000000
VERSION     DASVXS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126880"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VF752_17335"
     CDS             <1..361
                     /locus_tag="VF752_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8857361.1"
                     /translation="VVCLYVLVQALVLLARERRSTIAVLRAGGAGQREVRRVLAGASL
                     LVAVLAAPLAVLLELLLLGPATSRLAAGYVSLPLRASGMEIAAVALGLVGIALASAAI
                     AARRIGRETVVAGLASE"
     gene            373..1809
                     /locus_tag="VF752_17340"
     CDS             373..1809
                     /locus_tag="VF752_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857362.1"
                     /translation="MSPALLATIVLLAGCGAADPGSSGSTLQSTWVDPGSTGALRRGP
                     AEPFVQRTELASASRPVETLATLGQITDAHVRDEESPARVPFLDRLGAPFNSTFRPQE
                     ALSTQVLTSIVRSLDAERPQAVVETGDLIDNDQQNELAWAIAALRGGQVNPASGAPGY
                     DGVQRQDDPDPLFYRPEVDAPRHPGLLAAAQEPFRSPGLTAPWYPVLGNHDLLVQGEL
                     APTAATERVATGGRRVLSIDTSAVDLAQARRDPARAVDELLAGRIPRQTEATQPDSTR
                     RELPPGEVARRLIAGSGHGALRGGRLDYTFDVGSHLRAIVLDTIRRDSGSEGELSRAQ
                     VSWLRSALRSVGDRYVIVFTHQPLDSFAAGADALALLDRDAHVVATVAGNTHRNRIRP
                     RQSPSGGYWEITTSSLADWPQQARMLRLVRTASGRLALETWMVDGTGSKLADTARELA
                     YLDAQGGRPQRFAGTVGDRNAVLYLNAR"
     gene            1851..2693
                     /locus_tag="VF752_17345"
     CDS             1851..2693
                     /locus_tag="VF752_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8857363.1"
                     /translation="MDELRVAPEPASLPEQPAPSPVREDKPLFKRIGAGLAALVALIA
                     KFGAGLKWLLLLLPKAKIFTTAGTMLVSVAAYGLIWGWKFGVGFVLLLLLHELGHVFQ
                     LRREGVKASAPMFIPFMGAFIGAKSFGKDAAAEARVGLAGPILGSLATLVPLAIYLAG
                     GGDFFRALAFTGFFLNLFNLLPVVPLDGGRAMAALTPWMWLAGFLGLVALAVVFPNPI
                     VILIVLFAGLETFSRFRARKSPEAQRYHAVKPLTRLGVAAVYIGLAAALTLGMHFTHL
                     PRTF"
     gene            complement(2778..3704)
                     /locus_tag="VF752_17350"
     CDS             complement(2778..3704)
                     /locus_tag="VF752_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX8857364.1"
                     /translation="MRFCLMIEGQENVTWDNWRAIAAATEEAGLEALFRSDHYLSVQG
                     DPERGSLDAWATLAALAAVTSRIKLGTLVSPVTFRHPSVLAKNVVTVDHVSGGRVEVG
                     MGAGWHVAEHRAFGFPFPDTRTRMDMLEEQLEIVNGEWVEGAFSFDGRHYRLETVDAL
                     PKPVQRPRPNLIVGGGAGPRSAMLAARFADEYNTFMVSPEEFARRRGNVLRACEAVGR
                     EPLTLSLMTPVLVGRDSSEYEERRVRMAERRNESGFAPSAEWIAGTVEEAAETLRTYE
                     EAGVERVMFQQLLHDDLDMIDVIGRKLAPLFA"
     gene            complement(3714..4190)
                     /gene="smpB"
                     /locus_tag="VF752_17355"
     CDS             complement(3714..4190)
                     /gene="smpB"
                     /locus_tag="VF752_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX8857365.1"
                     /translation="MAKGKKRKAAPGDIATNRQASFRYNLLDKWEAGLQLQGSEVKSL
                     RDGKVQLKDAYATVDDGEVWLHNMHIAPYPAASRENHEPERTRKLLLHRGEIERLIGK
                     TAEKGLTLVPTRLYFKGRNAKVEIALARGKDVGDKRRAIKEREQKREIERALAERH"
     gene            complement(4204..5964)
                     /locus_tag="VF752_17360"
     CDS             complement(4204..5964)
                     /locus_tag="VF752_17360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEX8857366.1"
                     /translation="MTTAQRRGRAVALEPTVAVLSRRGRLTVAEPLFGRGPRVTLPGK
                     ALGDARVGDLVLVGGGKRGARVVRVLGRPDIARDVLEALMVDRGLRRAFPRSVELEAD
                     EREAEVGPHDEASRAGRRDLTDLPTFTIDPISARDFDDAISARREDARVRVWVHIADV
                     SAYVEPSRAIEAEAYRRGTSVYVPDAVEPMLPEALSNRACSLVPGEERLAVTVEMEMD
                     GTDAVSTSFYRSVIRSDERLNYQQVDRIFAGEERAEEPWAGPLEAARAVSAALREKRG
                     RRGALEVDSREPVFEFDKQGNVVAVVHEEQTESHELIEHLMILANEQVARYLSGRKVP
                     TLYRVHERPSPQAVEYLVEKLASLDVPTPPLPEDMTPQQAADAVGEISRMVAEHVRRT
                     GRGQAALTSLVLRSLKQAYYSPHNLGHAGLASTHYCHFTSPIRRYPDLVVHRGLLASL
                     GLDDHAPRAEDMEEAGLESSAAERHAMEIERASQDICQAFLLERALEESGYELDFEGE
                     VVGLIGAGAFVSFGDEGYEGFLPVRRMRGDWWSLNEEGTALEGERSGSVLRLGDPVGV
                     RVERVDTARGRVDLTPAASQ"
     gene            complement(5961..>6732)
                     /locus_tag="VF752_17365"
     CDS             complement(5961..>6732)
                     /locus_tag="VF752_17365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8857367.1"
                     /translation="SDVITKANGNSLAGESTDTATSKIKGPAGTSVTLTIVKGGKGPT
                     RTLTVKRKKLDIPLVEGKLVTRNGAKLGVDQLTQFSDGAHDKLRKQIDSQLKAGAKGI
                     VLDLRGNPGGLLQEGRMVASIFVPKGLIVSTKGRNSPAAKYNAVGGAISSKVPVVVLV
                     DRGSASASEITTGALKDHHRATVVGTRTFGKGVFQEVETLSNGGLLDLTVGRYFLPDG
                     ENIQKTGIEPDVQAKDDPNTKPDEALDKALSVLREKTG"
BASE COUNT          906 a         2435 c         2287 g         1104 t
ORIGIN      
        1 cgtcgtgtgc ctgtacgtgc tggtgcaggc gctcgtgctg ctcgcacgcg agcggcggag
       61 caccatcgcc gtgctccgag ccgggggcgc ggggcagcgc gaggtgcgtc gcgtgctcgc
      121 gggcgcgtcg ctcctcgtcg ccgtcttggc cgcgccgctc gcagtgctcc tcgagctgct
      181 gctgctcgga ccggccacca gccggctcgc ggcgggctac gtgtcgcttc cgctccgcgc
      241 gagcggcatg gagatcgcgg cggtagcgct cgggctcgtc gggatcgcgc tcgcgtcggc
      301 ggccatcgcg gcgcggcgaa tcgggcgaga gaccgtggta gcggggctgg cgagcgaatg
      361 aagatgcggc tcttgtcccc tgccctgctg gccacgatcg tgctgctggc cggctgcggc
      421 gcggccgatc cggggtcgtc cgggtccaca ctgcaatcga cttgggtcga tcccggctcg
      481 accggtgcgc ttcgccgcgg gcccgccgag ccgttcgtgc agcggaccga gctcgcatcc
      541 gcgtcacgcc cggtggagac gctggcgacc ctcgggcaga tcacggatgc acacgtgcga
      601 gacgaggagt ccccggcgcg cgtgcctttc ctggacaggc tcggcgcgcc gttcaactcc
      661 accttcaggc cgcaggaggc gctgtccacc caggtgctca cctcgatcgt gcggtcgctc
      721 gacgcggagc gcccgcaggc tgtggtggag acgggcgacc tgatcgacaa cgaccagcag
      781 aacgagctgg cgtgggcgat cgcggcgctg cgtggagggc aggtgaatcc cgcctccggt
      841 gcacccggct acgacggcgt gcagcgccag gacgacccgg atccgctgtt ctaccggccg
      901 gaggtggatg cgccgcgcca tcccgggctg ctcgccgcgg cgcaggagcc attccggtct
      961 cctgggctga ccgcgccgtg gtatccggtg ctcggcaacc acgacctcct cgtacagggc
     1021 gagctggcgc cgacggcggc caccgaacgc gtggcaaccg gcggccggcg cgtgctgtcg
     1081 atcgatacga gcgcggtgga tttggcgcag gcgcggcgtg accccgcccg tgccgtggac
     1141 gagctgttgg ccggccgcat tccgaggcag acggaggcca cccaaccgga ctcgacccgc
     1201 cgcgagctgc cgcccgggga ggtcgcacgc cggctcatag cgggcagcgg tcatggcgcg
     1261 ctgcgcggcg ggcggctcga ctacaccttc gatgtcggct cgcacctgcg cgcgatcgtg
     1321 ctcgacacga tccgcaggga ctcaggatcc gaaggtgagc tgtcgcgcgc gcaggtgtcg
     1381 tggctgcgct cggcgcttcg ctcagtgggc gatcggtacg tgatcgtgtt cacgcaccag
     1441 ccgctcgaca gcttcgctgc cggcgccgat gcgcttgcac tgctcgaccg cgacgctcac
     1501 gtggtggcga cagtcgcggg gaacacgcac cgcaaccgca tccggccgcg gcagtcgccc
     1561 tccgggggct actgggagat caccacctcg tccctagccg actggccgca gcaggcgcgc
     1621 atgctgcgac tcgtgcgaac ggcgtccggg cggctcgcgc tcgagacctg gatggtggac
     1681 ggcaccggca gcaagctcgc ggacaccgcg cgtgagctcg catacctcga cgcgcagggc
     1741 gggcgacccc agcgcttcgc cgggacggtg ggcgaccgta acgccgtgct gtacctgaac
     1801 gcgcggtaaa gccttccgga tttctcccca ctcgctgcca gaattaaggc atggacgagc
     1861 tccgtgtggc gcctgagccc gcgagtcttc ccgagcagcc ggcgccctcc ccagtccgcg
     1921 aggacaagcc cctcttcaag cgcataggcg ccggcctagc cgcgctcgtc gcgctgatcg
     1981 ccaagttcgg cgcagggctg aagtggctgc tgctcctgct gcctaaggcg aagatcttca
     2041 ccaccgcagg cacgatgctc gtctccgtcg cagcctacgg gctgatctgg ggctggaagt
     2101 tcggcgtcgg ctttgtgctt ctcttgctgt tgcacgagct gggccacgtg ttccagctgc
     2161 ggcgcgaggg ggtgaaggcc agcgcgccga tgttcatccc gtttatgggc gcgttcatcg
     2221 gagccaagtc gtttggcaag gacgcggccg cggaggcgcg cgtgggcctc gccggtccga
     2281 tcctgggctc gctcgcgacg ctcgtgccgc tcgcgatcta cctggcgggc ggcggcgact
     2341 tcttccgcgc cctggcgttc accggctttt tcctgaacct gttcaacctg ctgccggtcg
     2401 tgccgctcga cggcgggcga gcgatggcgg cgctcacgcc gtggatgtgg ctcgcaggct
     2461 tcttgggcct cgtcgcgctc gcggtcgtct tcccgaaccc gatcgtgatc ctgatcgtgc
     2521 tgttcgccgg cctcgagacc ttcagccgct tccgcgcacg gaagtcacct gaggcgcagc
     2581 gctatcacgc cgtgaagccg ctcacgcgcc tcggagtcgc ggccgtgtac atcggcttgg
     2641 ccgccgcgct gacgctcggc atgcacttca cgcacctgcc gcggacgttc taggcacccc
     2701 gcaactcctg actttggttc tcgtatgcgg aaccaaactc agcagttagc tcgggcccgg
     2761 tccgccggag tagcgactca agcaaacagc ggggccagct tccgcccgat cacatcgatc
     2821 atgtcgaggt cgtcgtgcag cagctgctgg aacatcaccc gctcgacgcc cgcctcctca
     2881 taggtgcgca gcgtctcggc cgcctcctcc accgtgccgg cgatccactc ggcgctcggg
     2941 gcgaagccgc tctcgttgcg gcgctccgcc atgcggacgc ggcgctcctc gtactccgag
     3001 ctgtcgcggc cgaccagcac cggcgtcatg agcgagagcg tgagcggctc gcgccctacg
     3061 gcctcgcagg ccctgagcac gttcccgcgc cgcctcgcga actcctcggg cgacaccatg
     3121 aacgtgttgt attcgtcggc aaagcgcgcg gcgagcatcg cgctgcgcgg gccggctccg
     3181 ccgccaacga tgaggttcgg ccgcgggcgc tgaactggct tcgggagcgc gtcaacggtc
     3241 tccagccggt aatgccgacc atcgaacgag aacgcgccct cgacccactc tccgttcacg
     3301 atctcgagct gttcctcgag catgtccatt cgcgtgcgcg tgtcgggaaa cgggaaaccg
     3361 aacgctcggt gctcggcgac gtgccagccg gcacccatgc ccacctccac gcgcccgccc
     3421 gaaacgtggt cgaccgtgac cacattcttc gcaagcaccg acgggtggcg aaacgtcacc
     3481 ggtgacacga gcgtgccgag cttgatcctc gaggtcacag cggccagcgc cgcgagcgtg
     3541 gcccacgcat cgagcgagcc gcgctctgga tcgccctgca ccgagaggta gtggtcgctg
     3601 cggaagagcg cctcgaggcc ggcctcctcg gtggccgccg cgatcgcccg ccagttgtcc
     3661 caggtgacgt tctcctgccc ctcgatcatc aggcagaaac gcatcgatcc ctcctagtgg
     3721 cgctctgcga gcgcgcgctc gatctcgcgc ttctgctcgc gctccttgat cgcgcggcgc
     3781 ttgtccccga catccttccc gcgcgcgagc gcgatctcga ccttggcgtt gcgccccttg
     3841 aagtagagcc gcgtcggcac gagcgtgaga cccttctcgg ccgtcttgcc gatcagccgc
     3901 tcgatctcgc cgcggtggag cagcagcttg cgcgtgcgct cgggctcgtg gttctcgcgc
     3961 gacgccgcgg gatacggcgc gatgtgcatg ttgtgcagcc acacctcgcc gtcgtcgacg
     4021 gtcgcgtagg cgtccttcag ctggaccttg ccgtcgcgca gcgacttgac ctccgagccc
     4081 tgcagctgga gtcccgcctc ccacttgtcg agcaggttgt agcggaacga cgcctgcctg
     4141 ttcgtggcga tgtcgcccgg cgccgccttg cgtttctttc cctttgccat cagccgattg
     4201 actctattgc gaagccgcgg gggtgaggtc cacgcgcccg cgcgcggtgt ccacgcgctc
     4261 gacgcgcacg cccacggggt cgccgaggcg cagcacggag ccgctcctct cgccttcgag
     4321 cgcagtgccc tcctcgttga ggctccacca gtcgccgcgc atgcgccgga ccggcaggaa
     4381 gccctcgtag ccctcgtcgc cgaacgacac gaacgcgccg gcgccgatca gtccgaccac
     4441 ctcgccctcg aagtcgagct cgtagcccga ctcctcgagc gcgcgctcga gcagaaacgc
     4501 ctgacagatg tcctgcgacg ctcgctcgat ctccatcgca tggcgctcgg ccgcggagct
     4561 ctcgagcccc gcctcctcca tgtcctccgc ccgcggcgcg tggtcgtcga ggcccagcga
     4621 ggcgagcagc ccgcggtgca ccacgaggtc gggatagcgg cggatcggcg aggtgaagtg
     4681 gcagtagtgc gtgctcgcga gcccggcgtg accgaggttg tgcggcgagt agtaagcctg
     4741 cttgagcgag cggagaacga gcgaggtgag cgccgcctgg ccccgcccgg tgcgccggac
     4801 gtgctcggcg accatgcggc tgatctcgcc gacagcatcg gccgcctgct gaggcgtcat
     4861 gtcctcgggg agcggcggcg tcggcacgtc cagcgacgcc agcttctcca ccaggtactc
     4921 gaccgcctgc gggctcggcc gctcgtgcac gcggtagagc gtcggcacct tgcggccgga
     4981 gaggtaacgg gccacctgct cgttggcgag gatcatcaag tgctcgatca gctcgtgcga
     5041 ctcggtctgc tcctcgtgga cgacggccac gacgttgccc tgcttgtcga actcgaacac
     5101 gggctcgcgc gaatcgacct cgagcgcgcc cctgcgcccg cgtttctcac gaagcgcggc
     5161 cgacacggcg cgcgcagcct cgagcggccc ggcccacggc tcctcagcgc gctcttcccc
     5221 agcgaagatg cgatccacct gctggtagtt cagccgctcg tccgatcgga tcaccgagcg
     5281 gtagaaggag gtcgacaccg cgtccgtgcc gtccatctcc atctcgacgg tgacggcgag
     5341 ccgctcctca cccggcacga ggctgcaggc gcggttcgac agcgcctccg gcagcatcgg
     5401 ctccaccgcg tccggcacgt acacgctcgt gccgcgccgg tacgcctcgg cctcgatcgc
     5461 gcggctcggc tccacgtacg cgctcacgtc ggcgatgtgc acccagaccc gcacgcgagc
     5521 gtcctcgcgc ctggcggaga tggcgtcgtc gaagtcccgg gcggagatcg ggtcgatagt
     5581 gaaggtgggc aggtccgtga gatcgcgacg acccgcgcgc gaggcctcgt cgtgcggccc
     5641 cacctcggct tcgcgctcgt cggcctcgag ctcgaccgag cgcgggaacg cgcggcgcag
     5701 gccgcgatcg accatcagcg cctcgagcac atcgcgtgcg atgtccggtc ggccgagcac
     5761 gcgcaccacg cgcgcgcccc gcttgccgcc gccaacgagt acgagatcgc cgacgcgtgc
     5821 gtcgccgagc gccttgcccg gcagcgtgac gcgcggaccg cgcccgaaca gcggctccgc
     5881 gaccgtcagc cgcccgcgcc gcgagagcac ggcgacagtc ggctcgagcg ccacggcccg
     5941 gccccggcgc tgcgccgtcg tcacccggtc ttctctcgaa gcacggaaag cgccttgtcg
     6001 agggcctcgt cgggcttcgt gttcggatcg tccttcgcct gcacgtccgg ttcgataccc
     6061 gtcttctgga tgttctcgcc gtccggtagg aagtaccgcc ccacagtcag gtccagtagg
     6121 ccgccgttcg agagcgtctc cacctcctgg aatacgccct tgccgaacgt gcgcgtgccg
     6181 acgacggtcg cgcggtggtg gtccttcagc gctcccgtgg tgatctcgga ggcgctggcg
     6241 ctgccgcggt cgacgagcac gacgacgggc accttcgagg agatggcacc cccgactgcg
     6301 ttgtacttcg ccgccggact gttgcggccc ttcgtggaca cgatcagccc cttgggcacg
     6361 aagatgctcg cgaccatccg cccctcctga aggagcccgc cggggttgcc acggagatcg
     6421 agcacgatgc ccttcgcacc ggccttgagc tgtgagtcga tctgcttgcg cagcttgtcg
     6481 tgggcgccgt cgctgaactg ggtgagctga tcgacgccga gcttggcgcc gttgcgcgtg
     6541 acgagcttgc cctccacgag cgggatgtcg agcttcttgc gcttgaccgt cagcgtgcgg
     6601 gtcggtccct tgccgccctt cacgatggtg agcgtgaccg aggtgccggc tggccccttg
     6661 atcttgctcg tcgcggtgtc ggtcgactcg ccggcgagcg agttgccgtt cgccttggtg
     6721 atcacatcgc tg
//
LOCUS       DASVXS010000310         8850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_127485, whole genome shotgun sequence.
ACCESSION   DASVXS010000310 DASVXS010000000
VERSION     DASVXS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127485"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF752_17370"
     CDS             <1..558
                     /locus_tag="VF752_17370"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX8857368.1"
                     /translation="VLDGERVLAPMRELGDPVADLMGPLPYVAMQGLLDALYPAGGSN
                     YFKAGFIDELSDAAIDTLVERHGAMNSPMSEIHVQHFGGAVRRVSPSATAFANRGAEY
                     VLNVLGRTPDSAGFDEAVDWARVTYDAMEPYTASGTYSNFMSAGDDRVNQAYPEDTYR
                     RLQALKDRYDPTNLFRLNQNVAPSG"
     gene            631..1689
                     /locus_tag="VF752_17375"
     CDS             631..1689
                     /locus_tag="VF752_17375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8857369.1"
                     /translation="MAFVSTAVSRPGVTGPRWADETFSRLKEEKALSQAFPSAASAAS
                     SVDISVAAWAILAVLVIGLLAVDLLVFARHHAPSLRESLHWSIGWLVLALAFGAGLWF
                     LQGAQAGSEYYAGYLLERSLSLDNVFVFAVILSYFAVPRDVQPSVLGWGIVLALALRL
                     VFILIGAALLDAFHATFYAFGILLLYTAYKLARHAELEIEPERNPALRFVRRFLPLTD
                     NYDGERLITRRSGRLLATPLFAVFVVVATMDVIFAVDSIPAIFAVTREPFIVFAANAF
                     AMLGLRALYFLIVRMRERFVYLSQGLAAILGFIGVKMLLVDVWHVPIWLSLTVIVAVL
                     ASAAALSARVAPRSKAAA"
     gene            complement(1696..2187)
                     /locus_tag="VF752_17380"
     CDS             complement(1696..2187)
                     /locus_tag="VF752_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857370.1"
                     /translation="MPSKYQAATAAAAAALTAAIAIPTVTGAQTGGRDVTVREKVRAA
                     QFVHAKDSTKGERLARGDRVITSQAMFDGDNRPLGTLYTDCVNAGRAARVFNATLQCT
                     STYRFSDGQVVSAGVVKLGSGPRGGFPIVGGSDAYKSARGQITPGAPAKGYDGVDVLH
                     LDG"
     gene            complement(2301..3449)
                     /locus_tag="VF752_17385"
     CDS             complement(2301..3449)
                     /locus_tag="VF752_17385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8857371.1"
                     /translation="MPKRGALARLTSKRWFDPALAAVLIAVPVVEIFATPNRPAPAAA
                     AVLMVLLGLAVAVRRTHPVAGVVAAAVVIVASAALPGKFPPDTAALASFVLSYSCGAH
                     ASRRAGLLGIVMLAIAMQARMGLADFPNVEILFTTLAPWWIGLQVMERRQIVGELAER
                     NRELEAEQDAFARLAVRKERARIARELHDIVAHHLAVMVVQAGAGRMAGEGSADRAAG
                     RFRTIHRSGEQALAEMARLVDILHADDAGQRRNRLQILLDQAGAGGLQLCVTPLPADV
                     QLPADVEQAAYHVLQEGLTNAMKHAPGAEVHVRLTVTAESLELELFNDRGGVASALVS
                     TGSGMGLAGIRERVESLGGEVAAEPVSGGGFRLHARLPLASRELTFAR"
     gene            complement(3442..4602)
                     /locus_tag="VF752_17390"
     CDS             complement(3442..4602)
                     /locus_tag="VF752_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857372.1"
                     /translation="MNTGAPRWQFPLDLGICAAMLAFAVPVTLQPDSPDGAGAGTVFD
                     TLLLPTVIAPILLRRRAPFGAAVALAAGCVISGIPTFDQFRLGVAIPAAMLVLYSLAS
                     RRDRWHAVIGLVLVLAGMVFIGLTDVVLTDEGGVGAMVVFSFPLCIGTWVAGRLVRSR
                     DQVAAELVERSHLLERQREQTAQLAVEVERTQLASDLDAAARVRVREMIDLAEVGERS
                     LATDHARAREAFGRLERMGRDSLNEMRGLLGVLRSDERGTRSPRPTLAQLETLIEEAR
                     AGGRAVDLEVAGERRPLPSGVELAAYRALQHALVAVHGADPEPATVQLRYLPSAVVLE
                     VSGLASEGSGAEAALVAARERVTAHGGTFSADTPPTGRCVLRARLPLVATGA"
     gene            4755..5969
                     /locus_tag="VF752_17395"
     CDS             4755..5969
                     /locus_tag="VF752_17395"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8857373.1"
                     /translation="MDNPFVTILFTDLVGSTALFDRHGDDAADALRRRHFVTLRDAVA
                     KHGGREIKSTGDGLMVAFGSAVSAVHCAVDIQRATTGDEGALSVRVGIDAGEPLPDDH
                     DLYGTPVIIASRLCDAAGEGEILASEVVCRIAGHRVAEPIVPVGAISLSGIAEPVATA
                     RVQWRDDEGDSRAEPEDHPRSISVVIADDQALVRAGFRAILEEQPGIAVVGEAADGRD
                     AIDLARKRKPAVVLMDIQMPDLDGIEATRRILEEADEIHPVAVLMLTTFDLNEYVYEA
                     LRAGASGFLLKDVMPEDLIAAVRVVAAGDGLIAPTITRRLIAQFARTAPPTAPPAGLE
                     ELTPRELEVLTLVARGRSNAEIADELVLSEATVKTHVKRILRKLDLRDRVQAVVVAYE
                     AGVVAPGDTTRT"
     gene            5971..6870
                     /locus_tag="VF752_17400"
     CDS             5971..6870
                     /locus_tag="VF752_17400"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX8857374.1"
                     /translation="MAHRASASRRALTPVARAAVRYGPTRVWRERAWSRARHAGLHEP
                     SRRFNSRTRFGFTIAGDQALIMPRCLHWFGQWEPLLTTWLRATLQPGDAFLDVGANTG
                     YYTMLASRAVGPSGSVVALEPSATVRSALEANLRRNRMTNVRVLRIAGADREGTAPLY
                     RAPWNDAETSTLPRHGHQREAEVPTAPVLRLPSEDELARMRVAKIDVEGGERAVLDGI
                     RSDIERAPALQHVAIEVHPAELAGQESSAHQLIAGFEPLGFGPSWLPVDFSEAAHLER
                     PSAVVPRLDDVPNDQLVHMILSR"
     gene            6873..7616
                     /locus_tag="VF752_17405"
     CDS             6873..7616
                     /locus_tag="VF752_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8857375.1"
                     /translation="MELFERVHLVASGSGGFDLTDPFDSHAYLVNGGSEAALVDAGIG
                     AAVDQLLANVERAGVARDAVSSLLLTHAHPDHCGGAAALKEVLPQLEVLASPHVADAV
                     ERADEAAMSLAAGKTAGFYPVDYSPRRCAIATRLGDGDQIRVGSVSLRALATPGHSAG
                     HLAYLGELGGRTACFCGDLVFYGGLISLENNLDCSLHDYAASVQRLEREWIDALLPGH
                     HQISAWRGHRHVETAARQFARGFVPRSVV"
     gene            complement(7632..8066)
                     /locus_tag="VF752_17410"
     CDS             complement(7632..8066)
                     /locus_tag="VF752_17410"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8857376.1"
                     /translation="MSTGLATNNKATFRRFNDAVNSGDMELISKMIDELVAPDVRIRT
                     PLPLKETGAQALKQVWATLLRAYPDLHVTVEDLLGEGDKLVCRNVVTGTHRGEYMGIP
                     PTGRSVTYNEMFIFRFEDGRVAETWGVVDVLSQLRQLGAIPV"
     gene            complement(8071..>8850)
                     /locus_tag="VF752_17415"
     CDS             complement(8071..>8850)
                     /locus_tag="VF752_17415"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8857377.1"
                     /translation="EQIASPRGFVATVTTRLAIDELRSARARREEYVGEWLPEPIITG
                     GHDDPARRAEMADSLSLAMLVLLESLSPEQRAVLLLHDVFDYGYPEIAQIVGKRQDNV
                     RQLATRARRHVEQRRPRFQTTGEQRDELARRFFAAAEQGDLAGLEALLAHDVELTGDG
                     GGKAPALARSLRGRNRVARAVVKWLRRATRAPGVSFRPVEVNGSPGALFLDAQQRLIG
                     VCALDMDAGEITSISGIVNPDKLTHLGPVGDLGSLLRSPRR"
BASE COUNT         1338 a         3184 c         2989 g         1339 t
ORIGIN      
        1 gttctcgacg gcgagcgcgt actcgcgccg atgcgcgagc tgggcgaccc ggtcgccgac
       61 ctcatgggtc cgctcccgta cgtggcgatg cagggcctgc tcgacgccct ctacccggcc
      121 ggtgggagca actacttcaa ggccggcttc atcgacgagc tctcggacgc ggcgatcgac
      181 acgctggtcg aacgccacgg cgcgatgaac tcgccgatgt cggagatcca cgtgcagcac
      241 ttcggcggcg ccgtccggcg cgtgtcgccg tcggcgacgg cgttcgcgaa ccgcggcgcc
      301 gagtacgtgt tgaacgtgct cggccggacg cccgactcag ccggcttcga cgaggccgtc
      361 gactgggcgc gtgttaccta tgacgccatg gagccgtaca ccgcgagtgg cacgtactcg
      421 aacttcatga gcgcgggcga cgaccgcgtg aaccaggcat accccgagga cacgtacagg
      481 cggcttcagg cgctgaagga tcgctacgac ccgacgaacc tgttccgcct gaaccagaac
      541 gttgcgccga gcggttgaac cgctcggcgc tcggcgcgga ataccgcgat tgctaggctg
      601 acccactcga agggaagtag cccctaacgg gtggcgttcg tcagcacggc cgtatctagg
      661 cccggcgtca ccggcccgag gtgggcggac gaaaccttct cccgactgaa ggaggagaag
      721 gccctgtccc aggcatttcc gtccgccgcc tcggcggcct ccagcgtcga catctccgtt
      781 gcggcctggg caatcctcgc cgtcctggtc attggcctgc tcgcggtcga cttactcgtc
      841 ttcgccaggc accatgcgcc gtcgctgcgc gagtcgcttc actggtcgat cggatggctc
      901 gtcctggcgc tggccttcgg cgccgggttg tggttcctgc agggcgcgca agcaggctcg
      961 gagtactacg ccggctacct cctcgagcgc tccctgtcgc tcgacaacgt cttcgtcttc
     1021 gcggtcatcc tgagctactt cgcggtcccg cgcgacgtgc agccgagcgt tctcgggtgg
     1081 ggaatcgtgc tggcgctcgc gctgcgcctc gtcttcatcc tcatcggcgc cgcgctgctc
     1141 gatgccttcc acgcgacctt ctacgcgttc gggatcctgc tgctgtacac cgcatacaag
     1201 ctcgcccgcc acgccgagct cgagatcgag cccgaacgca acccggcgct gcgcttcgtg
     1261 cgccgcttcc tgccgctcac ggacaactac gacggcgagc ggctgatcac gcggcggtcg
     1321 ggccgcctgc tcgccactcc gctcttcgcc gtcttcgtcg tcgtggcgac gatggacgtg
     1381 atcttcgccg tcgactcgat cccggcgatc ttcgctgtca cgcgcgagcc gttcatcgtg
     1441 ttcgccgcga acgcgttcgc catgctcggc ctgcgcgcgc tgtacttcct gatcgtccgg
     1501 atgcgcgagc gcttcgtgta cctctcccag ggcctcgccg cgatcctcgg cttcatcggc
     1561 gtgaagatgc tgctcgtcga cgtctggcac gtgcccatct ggttgtcgct cacggtgatc
     1621 gtcgccgtgc tcgcatcggc ggcggcactc tctgcgcgcg tcgcgccgcg ctcgaaagcg
     1681 gcggcgtgac gcccgtcacc cgtcgaggtg caggacgtcg acgccgtcgt agcccttcgc
     1741 cggggcaccg ggcgtgatct ggccacgcgc cgacttgtag gcatcgctac cgccgacgat
     1801 cgggaaaccg ccacgcgggc cggagccgag cttcacgaca ccggcgctca ccacctggcc
     1861 gtcactgaat cggtaggtcg acgtgcattg cagagtggcg ttgaacaccc gcgcggcgcg
     1921 ccccgcgttg acgcagtcgg tgtaaagcgt ccccaggggg cggttgtcgc cgtcgaacat
     1981 ggcctggctg gtgatgaccc tgtcgccgcg cgccaggcgc tcgcccttcg tcgagtcctt
     2041 ggcgtggacg aactgcgcgg cacgaacctt ctcgcgaacg gtgacgtcgc ggccgccggt
     2101 ctgggccccg gtgaccgtgg ggatcgcaat tgccgcggtc agagcggcgg cagcggctgc
     2161 tgtagctgct tggtacttgc tcggcatgag ttgcctcctt cgctcgttga acgaggccag
     2221 tcaatcgccg tgctggcact tcgtcgtcgc gcccgatggc gaacttgcgc tacccccacc
     2281 gaggtagacg cgagcgcgtg ttaccgcgcg aaggtgagct ctcggctggc cagtgggaga
     2341 cgagcgtgca gtcggaagcc gccgccgctc accggttccg cagcgacctc gccgcccagc
     2401 gactcgactc gctcgcggat ccctgccaag cccatcccgg aacccgtgct gaccagggcc
     2461 gacgcgacgc caccccggtc gttgaacagc tcgagctcga gcgattcagc agtgaccgtg
     2521 aggcgcacgt gcacctctgc tccaggcgca tgcttcatcg cgttcgtcag cccctcttgg
     2581 agcacgtgat aggcagcctg ctcgacgtca gctggaagtt gcacgtccgc ggggagcggc
     2641 gtcacgcata gctggagacc gcccgcgccc gcctggtcga gcaggatttg gagccggttg
     2701 cggcgctgcc cggcgtcgtc ggcgtgaagt atgtccacca ggcgtgccat ctcggcgagt
     2761 gcctgctcgc cactgcgatg gatggtccgg aaccgccctg ccgcgcggtc ggcggatccc
     2821 tcacccgcca ttcgcccagc gccggcctgg acgaccatca ccgcgaggtg gtgggcgacg
     2881 atgtcatgga gctcgcgggc gatacgcgcg cgctctttgc ggaccgccag gcgagcgaag
     2941 gcatcttgct cggcttccag ctcacggttg cgctcggcca gctcgccaac gatctgccgg
     3001 cgctccatga cctgaagccc gatccaccac ggcgcaagcg tcgtgaagag gatctcgacg
     3061 ttcgggaaat ccgccagtcc catccgcgcc tgcatcgcga tcgcgagcat cacgatcccc
     3121 agcaggccag cgcgtcgcga ggcgtgcgcg ccgcacgagt agctgaggac gaaggatgca
     3181 agcgcggcag tgtctggagg gaatttcccc ggcaaagcgg cgctggcgac gatcaccacc
     3241 gcggccgcaa cgacgcccgc gacgggatgg gtgcggcgca cggcgacagc aaggccgagg
     3301 agcaccatga ggacagcggc ggcggccgga gccggccggt ttggggtcgc gaagatctcg
     3361 acgaccggga ctgcgatgag caccgctgcg agcgcaggat cgaaccagcg cttcgaggtg
     3421 aggcgcgcga gcgcgccgcg cttaggcacc ggtcgccacg agcggcaggc gcgctcgaag
     3481 cacgcaccgc cccgtcgggg gtgtgtccgc gctgaaggtc ccgccgtgcg cggttacacg
     3541 ctcgcgggcg gccacgagtg ccgcctccgc gccgctcccc tcactcgcaa ggccgctgac
     3601 ctcgagcacc accgcgctcg gcagataacg cagctgcacg gtcgccggct cggggtccgc
     3661 gccgtggacc gccacgagcg cgtgctggag cgcacggtac gccgccagct ccaccccgct
     3721 cggaagcggc cgccgctcgc ccgcaacctc gaggtccacc gcccgcccgc ctgcgcgcgc
     3781 ctcctcgatc agcgtctcga gctgcgccag cgtcggcctc ggggagcgtg tcccgcgctc
     3841 atcgctccgc agcacaccga gtagcccgcg catctcgttc agggagtcgc gacccatccg
     3901 ctcgagccgc ccgaacgcct cgcgcgcacg cgcgtgatcc gtagcgagcg agcgctcgcc
     3961 cacctcggcc aaatcgatca tctcgcgcac gcgcacgcgg gccgccgcgt cgaggtcgga
     4021 cgcgagctgc gtgcgctcca cctcgaccgc cagctgcgct gtctgctcac gctgccgctc
     4081 gagcaagtgc gaccgctcga cgagctcagc ggccacctgg tcacgcgacc gcaccaaccg
     4141 gcctgccacc cacgtcccga tgcagagcgg gaacgagaac acgaccatgg cccccacccc
     4201 gccctcatcc gtcagcacga cgtccgtcag gccgatgaac accatccccg cgaggacgag
     4261 gacaagcccg atcaccgcgt gccagcgatc gcgacgcgag gcaagcgagt acaggacgag
     4321 cattgcggcc gggatcgcga ccccgaggcg gaactggtcg aaggtcggga tgccgctgat
     4381 gacgcagccc gcggccaggg cgaccgcggc cccgaacggg gcccgccgcc tgagcaggat
     4441 cggagcgatt acggtcggca acagcaacgt gtcgaacacc gtgccggccc cggcgccgtc
     4501 cggagagtcg ggctgaagcg tgaccggcac ggcaaaggcc agcatcgccg cgcatatgcc
     4561 caaatccagc ggaaactgcc accgcggggc gcccgtgttc acgcgccgag cgtaactccg
     4621 gtgcgttgcc gtgtacatac gcggccacgc tagctctggc acgcccgtcc gccatcatcc
     4681 ccaggggcga cctcggcctg acacctcagg atgatgcaac tcggcgcgtc agccgagatg
     4741 atgtccgagg cgcaatggac aacccattcg tcacgattct gttcaccgac ctcgtcggct
     4801 ccacggcgct gttcgaccgc cacggcgacg acgccgccga cgcgttgcga cgcaggcact
     4861 tcgtgaccct ccgggacgcc gtggcgaagc acggcggccg ggagatcaag tcgaccggcg
     4921 acggcctcat ggtcgcgttc gggagcgcgg tctcagcagt gcactgcgcg gtcgacatcc
     4981 agcgcgcgac gaccggtgac gagggagcac tttcggtgcg ggtgggcatc gacgccggcg
     5041 agccgctgcc ggacgatcac gacctctatg gcacgcccgt gatcatcgcg agcaggctgt
     5101 gcgacgcagc cggcgagggc gagatcctcg cttccgaagt ggtgtgccgg atcgcggggc
     5161 accgcgtcgc tgagccaatc gtgcccgtag gcgcgatctc cttgagcggc atcgccgagc
     5221 ccgtggccac cgcgcgggtg cagtggcgcg acgacgaggg cgactcgcgg gccgagcctg
     5281 aagaccaccc gcgctcgatc agcgtcgtca tcgccgacga ccaggcgctt gtgcgagccg
     5341 gcttccgcgc gatcctcgaa gagcagcccg gaatcgccgt cgtgggcgag gctgccgacg
     5401 ggcgcgacgc gatcgacctc gcgcgcaaac gaaagccggc cgtggtgctg atggacatcc
     5461 agatgcccga cctggacggg atcgaggcga cccggcggat cctcgaggaa gcggacgaga
     5521 tccatcccgt cgccgtgctg atgctcacca ccttcgacct gaacgagtac gtgtacgagg
     5581 cgctacgcgc aggtgccagc ggcttcctgc tgaaggacgt gatgcccgaa gacctgatcg
     5641 cagcggttcg ggtggtcgcc gccggagacg ggctgatcgc accgaccatc acccggcgcc
     5701 tgatcgcgca attcgcgcgc accgctcccc cgaccgcacc gccggcgggg ctcgaggaac
     5761 tcacgccccg cgagctcgag gtgctgacgc tcgtcgcgcg cggacgatcc aacgccgaga
     5821 tcgcggacga gctggtgctc agcgaggcaa ccgtcaagac ccacgtcaag cggatactcc
     5881 gcaagctcga cctgcgcgat cgggtccaag cggttgtcgt cgcatacgag gccggcgtcg
     5941 tggcgcccgg cgacacgacg cggacctgac gtggcccatc gcgcgtccgc gagccgccgg
     6001 gcgctcacgc cggtcgcgcg cgctgcggtg cgctacgggc ccacgcgcgt ctggcgcgag
     6061 cgggcatggt cgcgcgcccg acacgctggc ctgcacgagc cgtcgcggcg cttcaacagt
     6121 cgcacccggt tcggcttcac tatcgccggc gatcaggcgc tgatcatgcc gcgctgcctg
     6181 cactggttcg ggcagtggga accgctactg accacttggc tgcgcgcgac actgcagccg
     6241 ggcgacgcgt tcctcgatgt gggcgcgaac acgggctatt acacgatgct cgcctcgcgc
     6301 gccgtgggcc cgagcgggag cgtggtcgcg ctcgagccct cggcaacggt gcgcagcgcc
     6361 ctcgaagcca acctgcgccg caaccgtatg acgaacgtcc gggtcctgag gatcgcgggc
     6421 gctgaccgcg agggcaccgc cccgctctac cgtgcgccct ggaacgacgc cgagacctcc
     6481 accctcccgc ggcacggcca ccagcgcgaa gccgaagtgc cgaccgcgcc tgtgctgcgc
     6541 ctgccctccg aggacgagct tgcccgcatg cgcgtagcga agatcgacgt ggagggtggc
     6601 gagcgcgcag tgctcgacgg gatccgaagc gacatcgagc gggcgccggc gctccaacac
     6661 gtcgcaatcg aggtccaccc cgccgagttg gccgggcagg agagctcggc ccatcagctg
     6721 atcgcgggct tcgaaccgct cgggttcggg ccgtcctggc tacccgtcga cttctcggaa
     6781 gcggcccatc tcgagcgacc gtccgccgtg gtcccgcggc tggacgatgt gccgaacgac
     6841 caactcgtcc acatgatcct gtcccgctga cgatggagct gttcgagcgc gtgcatctcg
     6901 tcgcgagcgg ctcgggcggc ttcgacctga ccgacccgtt cgactctcac gcgtacctgg
     6961 taaacggcgg ttccgaggcc gcgctcgtcg acgcgggaat cggcgcagcg gtggaccagc
     7021 tgctcgccaa cgtcgagcgc gccggcgtcg cgcgcgacgc ggtcagctcg ctgctcctca
     7081 cccacgccca ccccgaccac tgcggcggcg cggcggcgct caaggaggtg ctgccgcagc
     7141 tcgaggtgct cgcgtcgcca cacgtggccg acgcggtcga gcgcgccgac gaggccgcga
     7201 tgagcctcgc agccggcaag accgcgggct tctatccggt cgactacagc ccgcgccgtt
     7261 gcgccatcgc gacgcggctc ggcgacggcg atcagatccg cgtggggagc gtgagcctac
     7321 gcgcgctcgc gactcccggg cacagcgccg gccatctcgc atacctcggc gagctgggcg
     7381 gccgcaccgc ttgcttttgc ggcgacctcg tgttctacgg cggcctgatc tcgctcgaga
     7441 acaacctcga ctgctcgctg cacgactatg cggcgagcgt ccagcgcctc gagcgcgagt
     7501 ggatcgacgc gctgctcccg ggccaccacc agatcagcgc atggcgcggg catcgacacg
     7561 tagagacggc ggcccgccag ttcgcgcggg ggttcgtgcc gcggagcgtc gtatgagctc
     7621 gccgggctgc gctagacggg aatggcgccc agctgtcgca gctgcgagag gacatccacg
     7681 accccccacg tctccgcgac tcggccgtct tcgaagcgga agatgaacat ctcgttgtac
     7741 gtgacggatc tgccggttgg tggaatgccc atgtactcgc cccggtgggt tccggtaacg
     7801 acattcctgc acacgagctt gtcgccctcc ccgagcaaat cctcgaccgt gacgtgaagg
     7861 tcggggtagg cccgaaggag ggtcgcccac acttgcttga gtgcctgcgc cccggtctct
     7921 ttgaggggca gtggcgtgcg gatccgcacg tcgggcgcga cgagctcatc gatcatcttc
     7981 gagatgagct ccatgtcgcc gctgttgacg gcgtcattga agcgtctgaa cgtcgccttg
     8041 ttgtttgttg ccaggccggt tgacatatgg ctatctcctg ggcgacctca gtagcgatcc
     8101 gaggtcgccg actggtccga ggtgcgtcag cttgtcgggg ttgacgatcc cgctgatgct
     8161 tgtgatctca ccggcgtcca tatcgagcgc gcacacgccg atcagccgct gctgcgcgtc
     8221 gagaaagagc gctcccgggc tgccgttgac ctcgacgggg cggaacgaca cgcccggagc
     8281 acgggtggcc cggcggagcc acttgacgac cgcgcgcgcg acacggttgc gaccgcgcag
     8341 cgaccgcgcc agcgccggag ccttgccgcc cccgtcgccg gtcagctcca cgtcgtgagc
     8401 gagcagcgcc tcgagcccgg cgagatcgcc ttgctcggcg gcggcgaaga accgccgtgc
     8461 cagctcgtcg cgctgctcac ccgtggtttg gaagcgcggg cggcgctgct cgacgtggcg
     8521 tctggcgcgg gtggcgagct ggcgcacgtt gtcctggcgc ttgccgacga tctgggcgat
     8581 ctccggatac ccgtagtcga acacgtcgtg cagcagcagc accgcgcgct gctcaggcga
     8641 gaggctctcc agcagcacca gcatcgccag cgacagcgaa tcggccatct ccgcgcgccg
     8701 ggccggatca tcgtggccgc cggtgatgat cggctccggc agccactcgc ccacgtattc
     8761 ctcgcgacgg gcgcgcgcgg agcgcagctc gtcgatcgcc agccgggtgg tcacggtggc
     8821 cacgaacccg cgcggcgatg cgatctgctc
//
LOCUS       DASVXS010000311         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_127887, whole genome shotgun sequence.
ACCESSION   DASVXS010000311 DASVXS010000000
VERSION     DASVXS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127887"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF752_17420"
     CDS             complement(<1..468)
                     /locus_tag="VF752_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857378.1"
                     /translation="MTRPRKPIVLACAAALTVVLGACGKQSIDVKEPPGTPVHSGAVL
                     FNERCSGCHTLTPAAARGSVTPGDVGSKERTDGPNLDYRRENLQSVLFAIRNGGFSGA
                     IMPANIVVGPQAQQVAEFVAKYAGSKSQGGGQSTSTSPSGSSSSSGGTSAGDTT"
     gene            complement(635..1003)
                     /locus_tag="VF752_17425"
     CDS             complement(635..1003)
                     /locus_tag="VF752_17425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8857379.1"
                     /translation="MCITAELQRLPEIRSFADTCARAFGFDEEVRYQIKMAASEAVAN
                     AVEHGSDGPSAQVRIRAVDEGGLLAFYVTDSGSFVPRIAPRGALPERGRGLAFLGQLM
                     DEVDIRPGPTGTTLRFAKRL"
     gene            1091..1573
                     /locus_tag="VF752_17430"
     CDS             1091..1573
                     /locus_tag="VF752_17430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX8857380.1"
                     /translation="MPSDLDTASYRTAVSHFATGVTIVTAVGDSGPSGLTTNAVCSLS
                     LDPVLMVVCLDLTSRTLAVVRDKRRLAINILAQGQQDLAAAFASKRPHPEKFDGVRWR
                     EADGLPVLDGVVAWLAGDVRELIPGGDHVMAVVSVTAAEAPGGEPLVYYRSGYHSLAS
                     "
     gene            complement(1783..2181)
                     /locus_tag="VF752_17435"
     CDS             complement(1783..2181)
                     /locus_tag="VF752_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857381.1"
                     /translation="MASNALAAGWGAVAWFRHEPSIWFWYVLRVAQGIVVVQVALGLF
                     LLIGEGKQAPEGLHVLYGVSPLVVTLFSEGMRVGAAERELEGIDDPDSLPRSEQIALA
                     RRVVLREMGIMTIGALLIVTLSLRALSTGL"
     gene            2278..2634
                     /locus_tag="VF752_17440"
     CDS             2278..2634
                     /locus_tag="VF752_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX8857382.1"
                     /translation="MLKSYLPALVFLLLGGGIGAAFTVLNGVLGPRRPNAVKIEPYEC
                     GLPSEVKRNFRFGISFYLIAMLFILFDIEVIFLYPIAVQLKAFGTFALVETLVFIFLL
                     LVGFVFVWRRGALEWT"
     gene            2625..3269
                     /locus_tag="VF752_17445"
     CDS             2625..3269
                     /locus_tag="VF752_17445"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX8857383.1"
                     /translation="MDVTRQKVGDRSNLRINQLRAQQMLRGEIPDEELEEYVESRVLL
                     TRLEKAQNWARANAVWPATFGLACCAIEMISVIGSPRNDLARFGAEVSRASPRQADML
                     ILSGRVSIKMAPVIRRLYDQMLEPKWVIAMGACASTAGMFNNYALVQGADKFVPVDVY
                     VPGCPPRPEALMYGIIKLQDKIKNQPDLGWRKRYNAEGTEERAQLVAAEDGEVA"
     gene            3262..3783
                     /locus_tag="VF752_17450"
     CDS             3262..3783
                     /locus_tag="VF752_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX8857384.1"
                     /translation="MPDATGLELIAQRVRENLGEEAVTGTNFHRARATLDVAPARVRD
                     VLAYLRDSDEEPFDRLMSVHGCDYLPAEPRFGVHYELLNMERPDRLNVRTRVGLEAPE
                     LPTVTDLYPGANYLEREIYDMFGVVFEGHPDMRRILMPEDYTGYPQRRDFPMGGEDVI
                     YTFNEDALPRWYD"
     gene            3776..>4272
                     /locus_tag="VF752_17455"
     CDS             3776..>4272
                     /locus_tag="VF752_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857385.1"
                     /translation="MTDQVGNGDSGQGIQSTGVDPLAVPYREREQSITLDRAGMEEPI
                     IGTHLQPESEFFTINMGPHHPATHGVLRLLLTLEGEVVRECTPIIGYVHTGIEKSCED
                     KSYWKVIPFIERMDYLSYFFNMQAFCGAVETMLELEIPPRATYLRTIHLELNRLHSHL
                     FWLGTS"
BASE COUNT          701 a         1484 c         1406 g          681 t
ORIGIN      
        1 ggtcgtgtcg ccggccgacg taccgcccga ggacgagctg gagccgctcg ggctggtgct
       61 ggtgctctgg ccgccgccct gcgacttgga gcccgcgtac ttcgcgacga actcggcgac
      121 ctgctgagcc tgtggcccga cgacgatgtt cgccggcatg atcgcgccgg agaagcctcc
      181 gttgcggatc gcgaagagca cgctctgcaa gttttccctg cggtagtcga ggttcggccc
      241 gtcggtgcgc tccttggagc cgacgtcgcc gggtgtgacc gacccgcggg cggccgcggg
      301 agtgagcgtg tggcaccccg agcagcgctc gttgaacagc accgcgcccg agtggaccgg
      361 ggttccgggc ggctccttca cgtcgatcga ctgcttgccg caggcgccca gaacgacggt
      421 gagcgcagcc gcgcaggcaa ggacaattgg cttcctcggc cgggtcacgg gcgggcatat
      481 tatccgcagc ccggaggaac cagccgcccg ccgccgaaag ccttctcgcc ccggcggcgg
      541 ccgtagacga cccgaccagc gcaggattac gcccgagtcg tctcatatcg gcggcgatca
      601 aaggccggcg ccggggcccc agcaactccg cgggtcaaag gcgcttggcg aagcgaagcg
      661 tcgtgccagt cgggccgggc cggatgtcga cctcgtccat cagctgcccg aggaaagcga
      721 gcccccgccc gcgctcggga agagccccgc gcggggcgat tcgtggcacg aagctgccgc
      781 tgtccgtcac gtagaacgcg agcagcccgc cctcatccac ggctctgatc cggacttggg
      841 ccgagggccc gtcggagccg tgctctaccg cgttcgcgac cgcctcgctc gccgccatct
      901 tgatctggta gcgcacctcc tcgtcgaacc cgaacgcccg cgcgcacgtg tcagcgaagc
      961 tgcgtatctc gggcaggcgc tgaagctctg cggtgatgca catctccgcg tcgcgaaatc
     1021 cttttccaga tagggtcacc gcattcgccc caattggcgt cgcgctgtcg acaggggccc
     1081 gctccgacac ttgccctccg acctcgacac cgcttcctac cgaaccgcgg tcagccattt
     1141 tgccactggt gtcacgatcg tcacagccgt gggcgatagc ggtccctccg gcctgacgac
     1201 gaacgcggtc tgctcgctgt cgctcgaccc ggtgctgatg gtcgtctgtt tggacctgac
     1261 atcgcgcaca ttggcagtgg tacgggacaa gcgccggctg gcgatcaaca tcctcgcgca
     1321 gggccagcag gatctcgccg cggcgtttgc gagcaagcgg ccgcacccgg agaagttcga
     1381 cggtgtcagg tggcgcgaag cggacgggct gccggtcctg gacggggtgg tggcgtggct
     1441 ggcgggcgat gtgcgcgagc tgatccccgg cggggaccac gtgatggctg ttgtttccgt
     1501 caccgcggcg gaggcgccgg ggggcgagcc gctcgtgtac taccgcagcg gctaccactc
     1561 gctcgcgagc taggcgcgct ccgttcgctt cgctactggt cggcgaggcc gcgccggttg
     1621 ggcactgagg ggacggctca ggcgaccttc gaagactttt ggggcctata gcagccaaaa
     1681 gtcttcaaac attgccacgg cgcccgatcc ctacccatca gtgctcatcg gcggcgcaca
     1741 cgagccccag ccccgtcgcg aactgatcgc cgccgcgccg gcctacagcc cagtggaaag
     1801 cgcccgcagc gaaagcgtga cgatcagcag cgcgccgatc gtcatgatcc ccatctcgcg
     1861 cagcaccacg cgccgcgcca gcgcgatctg ctccgagcgc ggcagcgagt ccgggtcgtc
     1921 gatgccctcg agctcgcgct cggccgcgcc cacgcgcatg ccctcgctga acagcgtcac
     1981 caccaggggc gagacgccgt agagcacgtg gagaccctcg ggcgcctgct tgccttcccc
     2041 gattagaaga aagaggccga gggcaacttg gacaacgacg atcccctgcg ccactcgaag
     2101 gacgtaccag aaccagatgg acggttcgtg ccggaaccac gccaccgcgc cccaccccgc
     2161 ggcgagggcg ttcgaggcca ccacggctat cccgacaacc tcgtgtatgt ggctcagaaa
     2221 gcgtcctttc gcactatgta gcaacttgtc acaaaggtgt tataacccgg tgccgccatg
     2281 ctgaagagct acctcccggc actcgtgttc ctgctgctcg gcggcggcat cggggcggcg
     2341 ttcacggtgc tcaacggagt gctcggtccg cgccggccga acgccgtgaa gatcgagccc
     2401 tacgagtgtg gcctgccgtc ggaggtgaaa cggaatttcc gtttcggcat ctcgttctac
     2461 ctgatcgcga tgctgttcat cctcttcgac atcgaggtga tcttcctcta cccgatcgcc
     2521 gttcagctga aggcgttcgg gacgttcgcg ctcgtcgaga cgctggtgtt catcttcctg
     2581 ctgctggtcg gcttcgtgtt cgtctggcga agaggcgcgc tcgaatggac gtgacccggc
     2641 agaaggtggg cgaccgcagc aacctgcgga tcaaccagct cagggctcag cagatgctcc
     2701 gcggggagat cccggacgag gagctcgagg agtacgtcga gagccgcgtc cttctcacga
     2761 ggctcgagaa ggcgcagaac tgggcccgcg cgaacgctgt ctggccggcc acctttggac
     2821 tcgcctgctg cgcgatcgag atgatctccg tgatcggctc gccgcgaaac gacctcgccc
     2881 gcttcggcgc cgaggtctcg cgcgccagcc cgcgccaggc ggacatgctg atcctgtccg
     2941 gccgcgtctc gatcaagatg gcgccggtca tccgccggct ctacgaccag atgctcgagc
     3001 cgaagtgggt gatcgcgatg ggcgcctgcg cgtcgacggc gggcatgttc aacaactacg
     3061 cgctcgtgca gggcgcggac aagttcgtgc ccgtggacgt gtacgtgcca ggctgcccgc
     3121 cgcgcccgga ggcgctcatg tacgggatca tcaagctcca ggacaagatc aagaatcagc
     3181 ccgacctggg ctggcgcaag cgctacaacg ccgagggcac cgaggagcgg gcccagctgg
     3241 tcgccgcgga ggacggcgaa gttgcctgac gccactggcc tcgagctgat cgcccagcgc
     3301 gtgcgcgaga acctgggaga ggaggccgtc accggaacga acttccaccg ggcccgcgcg
     3361 actctcgacg tcgcgcccgc gcgcgtgcgt gacgtgctcg cgtatctccg cgacagcgac
     3421 gaggagccgt tcgaccgcct gatgagcgtg cacggctgcg actacctgcc ggccgagccg
     3481 cgcttcggcg tgcactacga gctcctcaat atggagcggc ccgatcgctt gaacgtgcgc
     3541 acgcgcgtgg gactcgaggc gcccgagctg ccgaccgtca ccgacctcta tcccggcgcc
     3601 aactacctgg agcgcgagat ctacgacatg ttcggcgtgg tcttcgaggg gcaccccgac
     3661 atgcgccgca tcctgatgcc cgaggactac acgggctacc cgcagcgccg cgacttcccc
     3721 atgggcggcg aggacgtgat ctacacgttc aacgaggacg cccttccccg ctggtatgac
     3781 tgaccaggtc ggcaacggag acagcgggca gggcatccag tcgaccggcg tcgatccgct
     3841 cgcggtgccc taccgcgagc gcgagcagtc gatcacgctc gaccgcgccg ggatggagga
     3901 gccgatcatc ggcacccacc tccagccgga gtccgagttc ttcacgatca acatgggccc
     3961 gcaccacccg gccacgcacg gggtgctgcg cctgctgctc acgctcgagg gcgaggtcgt
     4021 gcgcgagtgc acgccgatca tcggctacgt ccacaccggc atcgagaaga gctgcgagga
     4081 caagagctac tggaaggtga tcccgttcat cgagcggatg gactacctct cctacttctt
     4141 caacatgcag gccttctgcg gggcggtcga gacgatgctc gagctcgaga tcccgccccg
     4201 cgcgacctac ctgcgcacga tccacctcga gctcaaccgg ctgcactcgc acctgttctg
     4261 gctcgggacc tc
//
LOCUS       DASVXS010000312        11822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_912692, whole genome shotgun sequence.
ACCESSION   DASVXS010000312 DASVXS010000000
VERSION     DASVXS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912692"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..688)
                     /gene="rfbC"
                     /locus_tag="VF752_17460"
     CDS             complement(125..688)
                     /gene="rfbC"
                     /locus_tag="VF752_17460"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX8857386.1"
                     /translation="MRELDTRLDGLVLIEADKHGDERGFFVETYRRSLYASFGIHDNF
                     VQDNQSRSSRGVLRGMHFQVEPGQAKLIRAARGSILDVVVDIRRGSPTFGQWEAFALS
                     DENALQIYVPIGFAHGFVVTSEFADVVYKVSSYYDAAFESGIAYDDPDVGVDWPDGLE
                     LHVSERDANAPRLADVADQLPFKYRRR"
     gene            complement(700..1821)
                     /gene="lhgO"
                     /locus_tag="VF752_17465"
     CDS             complement(700..1821)
                     /gene="lhgO"
                     /locus_tag="VF752_17465"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX8857387.1"
                     /translation="MDPPADLDVAVVGAGIVGLAVARELLERMPGMRVGVLEREDEVA
                     QGQTGRNSGVIHAGIYYAPGTLKARLCVEGAQALYDYCEACGIEARRCGKVIVAVDDS
                     ELPALDELERRGHANSVPGLRRLSASELREIEPHARGVAALHSPATGVVDFAEVARAL
                     ARDVEKLGGTITTGCAVEAVEATGARFTVACAGAGASRLAVASGAPADPRVVPFRGSY
                     MRLGSERAELVRSLIYPVPDPRLPFLGVHLSRHIEGEVMLGPTALLAGGLAWPGTWRV
                     VKRHWRAGVAELRRAASRRALAADASRLVPGIEASDLEPGFTGVRAQAVGRDGRLIDD
                     FVIDETPRALHVRNAPSPAATASLAIAREIADRVEAALG"
     gene            1874..3487
                     /locus_tag="VF752_17470"
     CDS             1874..3487
                     /locus_tag="VF752_17470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8857388.1"
                     /translation="MDSSRVKRLTLIASILGSSVVFLDGSVVNVALPAIRDDLHVSQS
                     TQQWVVEGYLLTLSSLILIGGSLGDLLGRRKVFLWGLIGFGITSLLCAVAPSGAALVG
                     ARALQGVAGALLVPASLALITATYPEHERGGAIGTWTAWTGVAFVIGPLAGGVLIDAI
                     SWRLVFAINIPLVIATVMVVRAGVQESAVATRVPIDYTGALLCMLGLAGPTFGLTEQS
                     TYGWSDPRVWAPIVVGLLILGAFVVYESRASHPMLQLDLFKRRNFSVANLTTLTLYAG
                     LGALTFFLVLFLQQVAGYSAIQGGLALLPITIVMFALSRRFGLLASKVGPRWLMGGGP
                     LIGAAGLLMLLMIDESVNYWTDVLPGVLVFSLGLSMTVAPLTTTVLQAVEPEHAGVAS
                     GVNNAIARLAGLLGIALIGAVIAAQFGSSLDSRLSGERLNTPERRTVQSAKQTPLAGD
                     QVVPAAQRPRLGPEVEGATVTAFHVGIGASALLVALGGVVSLVGLRPHVRRRREREAA
                     PVHAPGQCIDCGFEREPEAPRVAAPATQA"
     gene            complement(3478..3903)
                     /locus_tag="VF752_17475"
     CDS             complement(3478..3903)
                     /locus_tag="VF752_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8857389.1"
                     /translation="MAETGPLAPLSEALTSVGDRWTLLVVAALLDGPKRFGDLQEELG
                     GIASNVLSQRLRHLEQQAIVVARPYSERPPRFVYELTDSGRDLAGALRLLADWGARHR
                     EDAEPPRHGECGTPLEATWWCPTCERPVDSAEADELHYA"
     gene            3980..4873
                     /locus_tag="VF752_17480"
     CDS             3980..4873
                     /locus_tag="VF752_17480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX8857390.1"
                     /translation="MAVLDQIGSELKAAAERFGPSVVGLGRGWGLGSGVVLEEGRVLT
                     AAHNIRRDEVTVALADGRRETGRVAGVDPDLDIAVIEVDTGMAPAIEWAEDAAPALGT
                     PVVALANPGGRGLRATFGFVSSTGRSFRGPRGRRIRGVTEHTAPLPRGSSGGPLLDTS
                     GRLLGLNAVRVDGGLILAVPADQLLRDRAAALSRGETTGTPRLGVAVASPRAARRMRQ
                     AVGLPERDGLLVRGVEEGSPADRAGIERGDLIVAASGDPVDGIDSLHAALDRAAGGKL
                     GLSVVRGADEQDLEVDLGAAA"
     gene            4870..5850
                     /locus_tag="VF752_17485"
     CDS             4870..5850
                     /locus_tag="VF752_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8857391.1"
                     /translation="MTVSDRPIPTESEALDAYSQTVVSVAERVGPSVANLRVNRRTRA
                     GSMPAGAGSGVALTADGFILTSAHVVAGPRRHGRAAFTDGRELGFTIVGSDPLSDLAV
                     LRAEASDLTPARLGDAENLRVGQLVVAIGNPHGYAGSVTAGVVSALGRSLPARAGRTV
                     RVIDNVIQTDASLNPGNSGGALVDNHGNVIGVNTAVAGVGLGLAVPINEYTRRVIGSL
                     MTDGRVRRAWLGIAGGVRPLPPGARAELGNRTCVEVVEVIPGSPAELAGLRPEDLLVA
                     VDGEPVEGVEDLQRLMVAERIGKRVELSVIRHGRPQSVDLVPVELDSRTG"
     gene            5890..6708
                     /locus_tag="VF752_17490"
     CDS             5890..6708
                     /locus_tag="VF752_17490"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8857392.1"
                     /translation="MRRRRAAATAVLALLALAAYIGWTLIRVDHHGAQVEKWSLKSRL
                     LGRSIDEVGVVPKGGGRRPLLVLLHGRGGSPDGMLHQELFDALRDLGDRAPAVVVVNG
                     GDHSFYHDRRDGRWGSYVLREAVPDAVRRLGADPRRVAIGGNSMGGYGALLAASRDDR
                     FCAVGGHSAALWPEPGATAEGAFDDAADYARNDVYGTSTWNPAVWRGTRVWMDVGRDD
                     PFRQADQDFAAALRRAGVRVTEHVWPGDHSDDYFKKHLGRYIAWYARVLASCRR"
     gene            complement(6705..7196)
                     /locus_tag="VF752_17495"
     CDS             complement(6705..7196)
                     /locus_tag="VF752_17495"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX8857393.1"
                     /translation="MAYASKHGSTHEVADAVADTLRDEGLEVEVRDVSDVKDVSSYAN
                     AVIGSALYMGRIRPEARAFLKDHGKELAGGRLALFAMGPKDADEKSLDDGRKQLDANL
                     KKLGDVEPATVAIFGGVIDPDKLHFPFSRMPASDARDWDAISAWAREVAVSFRGAESA
                     TTR"
     gene            7503..8963
                     /locus_tag="VF752_17500"
     CDS             7503..8963
                     /locus_tag="VF752_17500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8857394.1"
                     /translation="MLRAFAAGRRARSFARVPAVACILLLLVLPAAPVSAALHPMGAP
                     PVDGPSSKDTRTISNASTPELAVYGVWAVISIGSAGIAWITLSWMVQAWRTPATLRRT
                     RFAGDAEPVEHSFSLLVPARHEEQVLGTTLDTLARLDHPDFEILAIIGHDDGATRAVA
                     EAAAARNDNVRVVIDHSWPKSKPKGLNTALPVCGGDVVGVFDAEDEVHPGLLRRVDRL
                     FQTSGAHVVQGGVQLMNYRSSWYSLRNVLEYFFWFSSRLHFHARQRFIPLGGNTVFVR
                     ADVLRAVGGWDPECLAEDCELGVRLSVLGAKTEVAYESEIVTREETPHSIRELVKQRT
                     RWSQGFLQVLRAGHWRRLPPRQRLLGAYTLATPFLQAFSGLLIPLAFATAFLLKAPVV
                     VVLITFLPLAPMLIILAVEIAALGQFCRSYGQRARLYDYVRLVLGFFPYQLLLSYAAV
                     RAAAREVFGVRNWEKTAHVGAHLESRQAPSDTPVNA"
     gene            8960..10558
                     /locus_tag="VF752_17505"
     CDS             8960..10558
                     /locus_tag="VF752_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857395.1"
                     /translation="MTAVQTPPSPPADLERLGLRPGAAPAPGVDPDAPGSVRSWLGRR
                     AASFALLAPVLALIGVVQVWGIGQFPARFDDEGTYVAQAWAVQHLGQLAHYTYWYDHP
                     PLGWIVLALLGWPMHAFDGPGTAIAAGRALLVPITLLTAALLYAFARRLGMRKGFAAL
                     AVLAFGLSPLALNYHRMVWLDNLAMPWLIGAFVLALTPGRRLWAYAGAGACFAGAVLT
                     KETVLLMLPALALAVWQSSERSTRRFCFAVFGATFLLLASIYPLYALIKGELLPGPGH
                     VSLLQGVEFQLNGRAPSGSIFKAGTLSNYYVDGWLTLDKLLLGAGLASSVPGLLSRRL
                     RVPAVAFLIQVLMMLRPGYLPAPYVIAMLPFAALLAAGAFDAIARWRPTWPSPRLSLA
                     GPVIAAVAVATTVVVAAPRWAREDRRQMTTNADQPMQTAAHWLVRHAPRSSTLIIDDT
                     LWVDLVRQGFPRRNVVWYYKLGTDGTVDAKYPRGWREFDYVVSSQMMRTDRTANVRAA
                     LRNSRLVAGFGRGTSRVEIRRIRG"
     gene            10555..10995
                     /locus_tag="VF752_17510"
     CDS             10555..10995
                     /locus_tag="VF752_17510"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8857396.1"
                     /translation="MSRRGFRELCRFGTVGASGYVVNLAAFAVATAAGTHHLGAATLA
                     FLVAVTNNFFWNRRWTFRASDGAAVGQAARFFVVSTAVFLVSAALLDAFVSAGLPALP
                     AQAVAIVAVTPLSFAANKLWCFRDARRRERAPRADGALSYVRSQ"
     gene            10992..>11822
                     /locus_tag="VF752_17515"
     CDS             10992..>11822
                     /locus_tag="VF752_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEX8857397.1"
                     /translation="MTAVRTRLSGVVAVVVAGIAVVAFAAPNTRSDPAREAAEHFLDR
                     YVLPSGRVVRRDQGGDTVSEGQGYALMLSATVGDRRRFERVWAWTRAHLQRPDGLFSW
                     LWRGGRVADRQPATDADIDIAHGLVLGADRFRTPRLRTEAAHIGRAVLARETVQAGGR
                     LVLVAGPWATRGRLTLNPSYFSPRGFAALARATRDPRFGRLEASSLAILRALSGSTRR
                     LPPDWAVLSPDGRVRPSPSPSGQRAAFGYDAVRAPIRMAAARARAARALAAGWWPRLE
                     R"
BASE COUNT         1533 a         4233 c         4236 g         1820 t
ORIGIN      
        1 aacacgcgtg aggcttgccg ccgggaggcg aacgcgtttg ctaaccggcg cagccacgcg
       61 caccacgtct cgggtaaggc gctgtcggtg atctaccgcg acatcctcac gcgccgggct
      121 tcgcctatct ccgccggtac ttgaacggca gctggtccgc cacatcagcc agccggggcg
      181 cgttcgcgtc gcgctcggac acgtgaagct caaggccgtc cggccagtcg actcccacgt
      241 ccggatcgtc gtaggcgatg cctgactcaa acgccgcgtc gtagtagctc gacaccttgt
      301 agaccacgtc cgcgaactcg ctcgtgacga cgaagccgtg ggcgaagccg atcggcacgt
      361 agatctgaag cgcgttctcg tccgagagcg cgaatgcctc ccactgcccg aacgtggggg
      421 agccgcgccg gatgtccacc accacgtcga ggatcgagcc gcgagcggca cggatgagct
      481 tggcctgccc cggctcgacc tggaagtgca tgccgcgcag cacgccgcgg ctcgagcgcg
      541 actggttgtc ctgcacgaag ttgtcgtgga tgccgaagct cgcgtagagg ctgcggcggt
      601 aggtctcgac aaagaagccg cgctcgtcgc cgtgcttgtc ggcttcgatc aggaccaggc
      661 cgtcgaggcg tgtgtcgagc tcacgcacgg ggcggcaggc taacccagcg cggcctccac
      721 ccgatccgcg atctcgcgcg cgatcgcgag cgaggccgtc gccgccggcg acggcgcgtt
      781 gcgcacgtgc agcgcgcgcg gcgtctcgtc gatcacgaag tcgtcgatca ggcgcccgtc
      841 gcggccgacc gcctgcgcgc gcacgccggt gaagccgggc tcgaggtcgc tggcctcgat
      901 cccgggcacg agtcgcgagg cgtcggcggc gagcgcccgg cgcgaggcgg ctcggcggag
      961 ttccgcgaca ccggcgcgcc agtgccgctt caccacccgc caggtgccgg gccacgcgag
     1021 cccgccggcc agcaacgcgg ttgggccgag catcacctcg ccctcgatgt ggcgcgacag
     1081 gtgcacgccg aggaacggca ggcgcgggtc gggcacgggg tagatcagcg agcgcacgag
     1141 ctcggcacgc tcggagccca agcgcatgta gctaccgcgg aacggcacga cgcgcgggtc
     1201 ggctggagca cctgacgcga cagcgagtcg cgaggcgccc gcacccgcgc acgcgaccgt
     1261 gaagcgtgcg ccggtcgcct cgaccgcttc aaccgcgcaa ccggtcgtga tcgtcccgcc
     1321 gagcttctcg acgtcacgcg ccagcgcccg cgcgacctcc gcgaagtcaa cgacgccggt
     1381 cgccggcgaa tgaagcgccg cgacgccgcg agcgtgcggc tcgatctcgc gaagctcgga
     1441 agcactcaat cgccgcaggc cgggcacgct gttggcgtgg ccgcggcgct ctagctcgtc
     1501 cagcgcggga agttcgctgt cgtcgacggc cacgatcacc ttcccgcaac gtcgtgcctc
     1561 gatcccgcac gcctcgcagt agtcgtagag cgcctgcgcc ccctccacac acagccgcgc
     1621 cttcagcgtc ccgggggcgt agtagatgcc ggcgtggatc acgccgctgt ttcgtccggt
     1681 ctgcccctgc gcgacctcgt cctcgcgctc cagcacgccc acgcgcatcc caggcatccg
     1741 ctcgagcaac tcgcgcgcta ccgccagtcc gacgatgcct gcgcccacta cggcgacgtc
     1801 tagatcggcg ggcgggtcca cggccccgca ccctaatccg gcccgtaccc cgggtgtgac
     1861 aacctcaccg ctggtggatt cgagtcgcgt caagcgcctg acgctcatcg ccagcatctt
     1921 ggggtcctcc gtcgtgttcc tcgacggcag cgtggtgaac gtggcgctgc ccgctatccg
     1981 cgacgacctc cacgtgagcc agtcgacgca gcagtgggtg gtcgagggct acctgctcac
     2041 gctttcgtcg ctgatcctga tcggcggctc gctcggcgac ctgctcggtc ggcgcaaggt
     2101 gttcctctgg ggtctgatcg gcttcgggat cacctcgctc ctctgcgccg tcgccccgag
     2161 cggcgcggcg ctcgttggcg cgcgtgcgtt gcagggcgtg gcgggcgcgc tgctggtgcc
     2221 ggcctcgctc gcgctgatca ccgcgaccta tcccgagcac gagcggggtg gcgcgatcgg
     2281 cacctggacc gcctggacgg gcgtcgcctt cgtgatcggc cccctcgcgg gaggggtgct
     2341 gatcgacgcg atctcctggc gcctcgtctt cgcgatcaac atcccgctcg tgatcgcgac
     2401 cgtgatggtg gtgcgcgccg gcgttcagga gagcgccgtg gcgacccgtg tgccgatcga
     2461 ctacacgggc gcgctcctgt gcatgctcgg cctcgccggg ccgacgttcg ggctcaccga
     2521 gcagagcacc tacggctgga gcgatccgcg cgtgtgggca ccgatcgtcg tgggtctgct
     2581 gatcctcggc gcattcgtgg tttacgagtc gcgcgcgtcg cacccgatgc tgcagctcga
     2641 cctgttcaag cgacggaact tcagcgtcgc gaacctcacc acgctcacgc tctacgccgg
     2701 tctcggcgcg ctcacgttct tcctggtgct gttcctccag caggtcgcgg ggtacagcgc
     2761 gatccagggc gggctcgcgc tgctgccgat cacgatcgtg atgttcgcgc tctcgcggcg
     2821 cttcgggttg ctcgcgtcca aggtcggccc gcgctggctc atgggcgggg ggccgctcat
     2881 cggggcggcc ggtttgctga tgctgctgat gatcgacgag tcggtgaact actggaccga
     2941 cgtcctccct ggcgtcctgg tcttctcgct cggcctctcg atgacggtcg cgccgctcac
     3001 tacgaccgtg ctccaggcgg tggagcccga gcacgccggc gtcgcgtcgg gcgtgaacaa
     3061 cgcgatcgcg cgcctggcgg gcctcctcgg cattgcgctg atcggcgcgg tcatcgcggc
     3121 gcagttcggc agcagcctcg actcgcgcct gtcgggagag cggctgaaca cgccggagcg
     3181 ccgcactgtg cagtccgcga agcagacgcc gctcgcgggg gaccaggtcg tgccggcggc
     3241 gcaacgaccg cggctcgggc ccgaggtcga gggcgcgacg gtgacggcct tccacgtcgg
     3301 catcggcgcg tccgctctgc tcgtggccct cggcggcgtc gtctcgctcg tcggcctccg
     3361 gccgcatgtc cggcgccggc gcgagcgcga ggccgccccc gtccacgcgc cgggtcagtg
     3421 catcgactgc ggcttcgagc gcgaaccgga agcaccgcgt gtcgcggcgc cggccactca
     3481 ggcgtagtgc agctcgtcgg cctcggctga gtccacggga cgctcgcacg tcgggcacca
     3541 ccacgtggcc tcgagcggcg tcccgcactc gccgtgacgc ggcggctcgg cgtcctcgcg
     3601 atgccgcgcg ccccagtccg cgagcagccg cagcgcgccc gcgaggtcgc ggcccgagtc
     3661 ggtcagctcg tacacgaagc gcggcgggcg ctcggagtag ggtcgcgcga ccacgatcgc
     3721 ctgctgctcg aggtgccgca gccgctggct cagcacgttg gaggcgattc ctccgagctc
     3781 ctcctgcaga tcgccgaagc gcttcggccc gtcgagcagc gccgccacca ccagcagcgt
     3841 ccagcggtcg ccgacgctcg ttagcgcttc ggacagcggt gcgagcggtc ccgtttcggc
     3901 catgcccgga gagtagccga gaagccggtg gtatggttgc aacttgcaat ctgcaagtca
     3961 ctgctgaatg aggcttgaaa tggctgttct ggaccaaatt ggaagcgaac tcaaggcggc
     4021 tgccgagcgt ttcggcccct ccgtcgtcgg actcgggcgg ggctggggcc tcggctcggg
     4081 cgtcgtgctc gaggagggcc gcgtgctcac ggccgcgcac aacatccgcc gcgacgaggt
     4141 gacggtcgcg ctcgccgacg gtcggcggga gaccggccgc gtggccggcg tggacccgga
     4201 cctcgacatc gcggtgatcg aggtcgacac cggtatggcg ccggcgatcg agtgggccga
     4261 agacgccgcg cccgccctcg gcactcccgt ggtcgcactc gcaaacccgg gtggacgcgg
     4321 gctgcgcgcg accttcggct tcgtgtcgtc gaccggtcgc agcttccgcg ggccgcgcgg
     4381 gaggcgcatc cgcggcgtga ccgagcacac cgcgccgctc ccgcgcggat cgtcgggcgg
     4441 accgctgctt gacacctccg gacgactgct cggactgaac gctgtgcgcg tcgacggcgg
     4501 cctgatcctc gccgtccccg ccgaccagct gctgcgcgac cgcgccgccg ccctctcgcg
     4561 cggggagacg acgggcacgc cgcgcctggg cgtggcggtg gcgtcgccgc gcgccgcgcg
     4621 gcgcatgcgc caggcggtcg ggctgcccga gcgcgacggc ctgctcgtcc gtggcgttga
     4681 ggaggggagc ccggcggacc gcgccggcat cgagcgcggt gacctcatcg tggccgcttc
     4741 gggcgacccc gtggacggca tcgactcgct gcacgcagca ctcgacaggg ccgccggcgg
     4801 caagctcggc ctcagcgtgg tgcgcggagc cgacgagcag gatctcgagg tcgatctcgg
     4861 agcggcggca tgaccgtgtc agaccgtccc atcccaaccg aatcagaagc gctcgatgcc
     4921 tattcgcaga ccgtggtgtc ggtcgccgag cgcgtcgggc cgtccgtcgc gaacctgcgc
     4981 gtgaaccggc gcacccgcgc gggcagcatg cccgccggag ccggcagcgg cgtggcgctc
     5041 acggcggacg gtttcatcct tacctccgcg cacgtggtcg ccgggccgcg aaggcacggc
     5101 cgcgccgcgt tcaccgacgg gcgcgagctc ggcttcacga tcgtcggcag cgacccgctg
     5161 tccgacctcg cggtgctgcg cgctgaggcg tccgacctca cgcctgcgcg gctgggcgac
     5221 gcggagaacc tgcgcgtcgg ccagctcgtg gtcgcgatcg ggaacccgca cggctacgcg
     5281 ggctcggtca cggccggcgt ggtctccgcg ctcgggcgct cgctgcccgc ccgcgccggc
     5341 cggaccgtcc gtgtgatcga caacgtgatc cagacggacg cttccctcaa cccgggcaac
     5401 tcgggtggcg ccctcgtcga caaccacggc aacgtgatcg gcgtgaacac cgcggtcgcg
     5461 ggcgtgggcc tcggcctcgc cgtcccgatc aacgagtaca cgcgccgcgt gatcggctcg
     5521 ctgatgaccg acggccgggt gcgccgcgcc tggctcggca tcgccggtgg tgtccggccg
     5581 ctgccgccgg gtgctcgcgc cgagctcggc aaccgcacgt gcgtcgaggt ggtcgaggtg
     5641 atccccggca gcccggcgga gctcgcgggc ctgcggcccg aggacctgct cgtggcggtc
     5701 gacggcgagc ccgtcgaggg cgtcgaggac ctccagcggc tgatggtcgc cgagcgaatc
     5761 ggtaagcgcg tcgagctctc ggtgatacgc catggtcggc cgcagtcggt cgatctcgtc
     5821 ccggtggagc tcgacagccg caccggttag cctcgcgcga tcttcgagag tcgcgagcga
     5881 cagagagcgg tgcgaaggcg gcgggcggca gcgaccgccg tcctcgcgct gctcgcgctg
     5941 gccgcctata tcggctggac gctcatccgc gtcgaccacc acggcgcgca agtcgagaag
     6001 tggtcgctga agagccgcct gctcggccgc tcgatcgacg aggtcggcgt ggtgccgaag
     6061 ggcggcggca ggcggccgct gctggtgctg ctgcacggcc gcggtggctc acccgacggg
     6121 atgctccacc aggagctgtt cgacgcgctg cgcgacctgg gcgaccgcgc gcccgcggtg
     6181 gtcgtggtca acggcggcga ccacagcttc taccacgacc gccgcgacgg ccgctggggc
     6241 agctacgtgc tgcgcgaggc ggtgccggat gcggtccgac ggctaggcgc cgacccgcgc
     6301 cgcgtcgcga tcggcggtaa ctcgatgggc gggtacggag cgctgctggc ggcgtcgcgc
     6361 gacgaccgct tctgcgcagt gggcgggcac tcggcggcgc tctggcccga gccgggtgcg
     6421 acggccgagg gagcgttcga cgacgcggcg gactacgcgc gcaacgacgt ctacggcact
     6481 tcgacgtgga atcccgccgt atggcgcggc acgcgcgtgt ggatggacgt gggccgcgac
     6541 gacccgtttc gtcaggccga ccaggacttc gctgcggccc tgcgccgcgc cggcgtgcgg
     6601 gtgaccgagc acgtttggcc cggcgaccac agcgacgact acttcaagaa gcacctgggg
     6661 cgctacatcg cctggtatgc gcgcgtgctg gcgagctgcc ggcgctagcg cgtcgtcgcg
     6721 gactcggcac cacggaagct cactgcgacc tcgcgcgccc aggcgctgat ggcgtcccag
     6781 tcgcgagcgt cgctggcggg catgcggctg aacgggaagt gcagcttgtc ggggtcgatc
     6841 acaccgccga agatcgcgac cgtcgccggc tcgacatcgc ccagcttctt caggttggcg
     6901 tcgagttgct ttcgcccgtc gtcgagcgac ttctcgtcgg cgtccttggg tcccatggcg
     6961 aagagcgcga gccgtccacc ggcgagctcc ttgccgtggt ccttcaggaa agcgcgcgcc
     7021 tcgggccgga tccggcccat gtagagcgcg ctgccgatca cggcattggc gtaggaggag
     7081 acgtctttca cgtcggacac gtcgcggacc tcgacctcga gcccctcgtc ccggagcgtg
     7141 tcggcgacag cgtcggccac ctcgtgggtc gacccgtgct tgcttgcgta tgccaccagt
     7201 gtcgtcgtaa ccaaagcgaa gcacccggac tcgcctgtgg catcggagct gcaccgggtt
     7261 tcagtccgtg gaaatccgca acccggttag cgcaagcgac gcggagtaat ccgtggctac
     7321 accaagtgcg tggttgccgg ccgttgcgtg aactacgtca cgtttggaat cgggtgaggg
     7381 ggaacagacg acgcgtggct cggcgtcgtc gagccgcatt tcctcccgca cccgactcgt
     7441 aaaagacatc tgcgcgcgca ggggcgccgt ggcgcgcgga tcacagaagg agaaggactc
     7501 gaatgttgcg agctttcgca gcaggccggc gtgcgcggtc gttcgcgcgg gtgccggccg
     7561 tcgcatgcat cctgttgctg ctggtgctgc cagctgcgcc tgtgtcggcg gcgctgcacc
     7621 ccatgggtgc cccgccggtc gacggcccct cctcaaagga cacgcggacg atttcgaacg
     7681 cgtcgactcc cgagctcgcc gtctacggcg tgtgggcggt gatttcgatc ggctccgccg
     7741 ggatcgcgtg gatcacgctc tcgtggatgg tgcaggcgtg gcgaacgccc gccacgctgc
     7801 ggcgcacacg cttcgccggt gacgcggagc ccgtcgagca ctccttctcg ctgctggtgc
     7861 cggcccgcca cgaggagcag gtgctcggca ccactctcga cacgctcgcg cgcctcgacc
     7921 atccggactt cgagatcctc gccatcatcg gccacgacga cggggccacc agggcggtgg
     7981 ccgaggccgc agctgcgcgc aacgacaacg tgcgcgtggt gatcgaccac agctggccga
     8041 agagcaagcc gaaggggctg aacaccgcgc tgcccgtatg cggcggggac gtggtgggcg
     8101 tgttcgacgc cgaggacgag gtgcaccccg ggctgctgcg acgcgtggac cggctgttcc
     8161 agacgagtgg tgcgcacgtg gtacagggcg gagtgcagct catgaactac cgctcgagct
     8221 ggtactcgct gcgcaacgtg ctcgagtact tcttctggtt ctcgagccgc ctccacttcc
     8281 acgcgcgaca gcgcttcatc ccccttggcg gcaacacggt tttcgttcgc gccgacgtgc
     8341 tgcgcgcagt cggcggctgg gatccggagt gcctcgctga ggactgcgag ctcggggtgc
     8401 gcctgagcgt gctcggcgct aagaccgagg tggcgtacga gtcggagatc gtcacgcgtg
     8461 aggagacgcc gcactccatc cgcgagctgg tgaagcaacg gacgcgctgg agccagggct
     8521 tcctgcaggt gctgcgcgcg gggcattggc gaaggctgcc gccccgccaa cgcctgctcg
     8581 gcgcctacac gctcgcgacg ccgtttctcc aggcgttctc cgggctgctc atcccgctcg
     8641 cctttgcaac agcgttcctg ctcaaggcgc ctgtggtggt cgtgctgatc acgtttctgc
     8701 cgctcgcgcc gatgctgatc atcctcgcgg tcgagatcgc cgcgctcggg cagttctgca
     8761 ggagctacgg gcagcgtgcg cggctgtacg actacgtccg gctggtgctc ggcttcttcc
     8821 cgtaccagct gctgctgagc tacgccgccg tacgcgccgc ggcgcgcgag gtcttcggcg
     8881 tccgcaactg ggagaagacg gcgcacgtgg gcgcgcacct cgagtcgcgc caggccccgt
     8941 ccgacacgcc ggtgaacgca tgacggcggt tcagacgccg ccgagcccgc cggccgacct
     9001 cgagcggctc gggctgcggc ccggcgccgc gcctgcgcct ggggtcgatc cagacgcgcc
     9061 gggcagtgtt cggtcgtggc tcgggcggcg cgcggcgagc ttcgcgctgc tcgctcccgt
     9121 gctcgcgctc atcggcgtcg tgcaggtgtg ggggatcggc cagtttccgg cgcgcttcga
     9181 tgacgagggc acttacgtgg cgcaggcctg ggccgtgcag cacctcggcc agctcgcgca
     9241 ctacacgtat tggtacgacc atccgccgct cggctggatc gtgctcgcgc tgctcgggtg
     9301 gccgatgcac gcgttcgacg ggcccggcac cgccatcgcg gcggggcgcg ccctgctcgt
     9361 gccgatcacg ctcctcactg cggcgcttct gtacgcgttc gcgcgccggc tcgggatgcg
     9421 caagggcttt gcggcgctag ctgtgctcgc cttcgggctc agcccgctgg cgctcaacta
     9481 ccaccgcatg gtgtggctcg acaacctcgc catgccgtgg ctgatcggcg cgttcgtgct
     9541 cgccctcaca ccgggaaggc gcctgtgggc gtacgcgggc gcgggcgcct gcttcgccgg
     9601 cgcggtcctc accaaggaaa cggttctgct catgctcccc gcgctcgcgc tcgccgtgtg
     9661 gcagagcagc gagcggagca cgcggcgctt ctgcttcgcg gtgttcggcg ccacgttcct
     9721 gctgcttgcc tcgatctacc cgctgtacgc gctgatcaag ggcgagctcc tgcccggccc
     9781 cggtcacgtg agcctgctcc agggcgtcga gttccagctc aatggacgcg cgccgagcgg
     9841 cagcatcttc aaggccggga cgctctcgaa ctactacgtg gacggctggc tcacgctcga
     9901 caagctgctg ctcggcgcgg gcctcgcgtc atcggtgccg gggctgctgt cccggcggtt
     9961 gcgcgtcccg gcggtggcgt tcctgatcca ggtcctgatg atgctgcgcc ccggctacct
    10021 gccggcgccg tacgtgatcg cgatgctgcc gtttgccgcc ctgctcgccg cgggcgcgtt
    10081 cgacgcgatc gcgcgctggc ggccgacttg gccgtcgccg cggctgtcgc tcgcgggccc
    10141 ggtgatcgcg gcagtggctg tcgcgacgac ggtcgtggtt gccgcccccc ggtgggctcg
    10201 agaggaccgg cggcagatga ccaccaacgc cgaccagccg atgcagaccg cagcgcactg
    10261 gctcgtgcgc cacgctccgc ggagcagcac gctgatcatc gacgacaccc tctgggtcga
    10321 cctcgtgcgc cagggctttc cacggcgcaa cgtggtctgg tactacaagc tcggcaccga
    10381 cgggacggtc gacgcgaagt atccgcgcgg ctggcgcgag ttcgactacg tggtgtcgag
    10441 ccagatgatg cgcaccgacc gcacggcgaa cgtgcgggcc gcgctgcgca actcgcgcct
    10501 cgtcgcgggc ttcggcaggg gcacgagccg ggtcgagatc cggaggattc gcggatgagt
    10561 cgacgcggct tcagagagct ctgccggttc gggacggtcg gcgcctccgg gtacgtcgtg
    10621 aacctggccg ctttcgccgt ggccaccgcg gcgggaacgc atcacctcgg ggccgcgacg
    10681 ctggcgttcc tcgtcgcggt cacgaacaac ttcttctgga accggcgctg gaccttcagg
    10741 gccagcgacg gcgccgcggt cggtcaggcc gcgcgcttct tcgtcgtcag caccgccgtg
    10801 ttcctcgtgt cggcggcgct gctcgacgcc ttcgtgagcg cgggcttgcc ggcgctgccc
    10861 gcccaggccg tcgcgatcgt cgccgtgaca ccgctcagct tcgccgccaa caagctctgg
    10921 tgcttccgcg acgcacggcg cagagagcgg gcgccgcgtg ccgacggggc actgtcgtac
    10981 gtcaggagcc agtgaccgcg gtccgcacac gactgtcggg cgtcgttgcg gtcgtggtcg
    11041 cgggcatcgc ggtcgtggcg ttcgccgcac cgaacacgcg gtcagatccc gcgcgcgagg
    11101 cggccgagca cttcctggac cgctacgtgc tgccttcggg ccgcgtcgtc cggcgcgacc
    11161 agggcggcga cacggtgagc gagggccagg gctacgcgct catgctgagc gcgaccgtgg
    11221 gcgaccgccg ccgcttcgag cgcgtctggg cgtggactcg cgcccacctc cagcgacccg
    11281 acgggctctt ctcctggctg tggcgcggtg ggcgcgtcgc ggacaggcag cccgccaccg
    11341 acgcggacat cgacatcgcc cacggactcg tgctgggcgc cgaccgcttc cgaacgcccc
    11401 ggctgcgcac cgaagcggcg cacatcggac gggccgtcct cgcgcgcgag accgtgcagg
    11461 cgggcggccg gctggtgctc gtggccgggc cgtgggccac gcgcggaagg ctgacgctga
    11521 atccgagcta cttctcgccg cgcggatttg cggcgctcgc tcgcgcgacc cgagacccgc
    11581 gcttcggccg gctcgaggcc tcgagcctcg cgatcctgcg cgcgctatcc ggctcgacgc
    11641 ggcgcctccc gcccgactgg gcggtgctgt cgcccgatgg cagagtgcgc ccgtcgccga
    11701 gtccgagcgg gcagcgggcc gccttcggct acgacgcggt acgagcaccg attcgcatgg
    11761 cggccgcgcg ggcgcgcgcg gcgcgggcgc tcgctgccgg ctggtggccg cggctcgaga
    11821 ga
//
LOCUS       DASVXS010000313        22776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_624471, whole genome shotgun sequence.
ACCESSION   DASVXS010000313 DASVXS010000000
VERSION     DASVXS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 22776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22776
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624471"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VF752_17520"
     CDS             <1..242
                     /locus_tag="VF752_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857398.1"
                     /translation="QAAKDFRWLPAIADAVNNSVPKPKTPDEPKFEDILGTELNKALV
                     EAIQKKSGYQAIAQKHLTTAANEMTKFIKQQGGYV"
     gene            284..1189
                     /locus_tag="VF752_17525"
     CDS             284..1189
                     /locus_tag="VF752_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8857399.1"
                     /translation="MPRRGRRRSDFWARVLASPWLLLTPAALALAAVALYPLVYGIRA
                     SFHKYRYGRDVGSAGFENYKDVFQDHTFWTAMGTTAKYVALAVSVETLLGLGLALLVS
                     NQLRFSRFFRVTLILPMTIAPVVVGVIWRLMYASDIGVVNPLFNAFGLSDPNVLANPG
                     SAFLGVVVVDIWEWTPLLFLIILAGLQSLPQEPLEAARVDGAGPVRVFFDHTLPLLRP
                     VLMVAVVLRLIDAIGTFDQIFVLTKGGPGTATQLISTYSYNTAFLFTQYGRAMAMVVS
                     LLVLVLILMVVAIRYMRSAAMRTAS"
     gene            1186..2016
                     /locus_tag="VF752_17530"
     CDS             1186..2016
                     /locus_tag="VF752_17530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8857400.1"
                     /translation="MTRERGISYARVATLLLAIVVALFPFWWMLVTSFKQPVDIFKGV
                     SLVPQHPTGDNYNRLFSNYHMGDFLVNSLIVVTVAVAVSLVLGTLAAYALARYRLRFH
                     LNESALVVSLLVRTLPAILLVIPLYLTLSKAGLLNTRLGLIIIYTGLNTSFVIWMMQS
                     FLEEIPKEIEEAAMVDGDSRLRALRRVVLPLAGPGLAATAIFSVIVTYNDFLLALTVT
                     STPSAQTVPVGVSTLIGKIQIEWGPMAAAGVVGALPIMIFAILVQRHFVRGLTLGAVK
                     "
     gene            2013..2843
                     /locus_tag="VF752_17535"
     CDS             2013..2843
                     /locus_tag="VF752_17535"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX8857401.1"
                     /translation="MSAEAPVLAACEWIFAGQEVPALLRRLSAAGCNGIEVSGEPARP
                     DRAELAAALEEAGVQATGITAICPAPTDERDLSHPDASARRRAVEYYRGCVDLARELR
                     APTVGLIPAAIGRVDGSVTESWPLAVEAAREVADYAGEHGVVLGVEAVNRYETFLVNT
                     ADQALHFAQEIGNAGVVLDAFHMQLEEEDSVAAVVRAAPRLRALHLADSNRLGLGHGQ
                     LDAAPLVAAARGAGFSGPFVFEFTAPGPNPFNPDKGEDAMRELDVYARESVAAVEAAL
                     "
     gene            2840..4267
                     /locus_tag="VF752_17540"
     CDS             2840..4267
                     /locus_tag="VF752_17540"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8857402.1"
                     /translation="MSERAMFIGGEWATGDARQDVLNPYSGEVIATVPRAGAEDADRA
                     VAAAVEGARQMRALPAHERAAILERAADIADERVDEMARTIATETGKALSEARGEAGR
                     AGGILRVSAHEGATAAGEVLPLDASPGGEGKLGFTLRQPCGVVVAITPFNYPLLLVLH
                     KVGPALAAGNAVVLKPAEATPLTALLLCEILLEAGLPPLALSVITGSGSELGPLLCAD
                     SRVRKISFTGSTRVGEEITRVAGVKRLSLELGATCPTLVLADADIDAAAAAIATGGYV
                     NAGQVCISVQRVIVQGPVHGDFLDALLPRVEAIEAGDPLGGSATIGSLISEREAERVE
                     RTIAEAAGNGARVLVGGDRDGAVVSPAVVDGVEPSAPLSRDELFGPAVAVTRVADVDE
                     AIRVANDSSYGLGAGVFTNDVTAAMRFVREVDAGALHVNWTPLWRADPMPYGGLKGSG
                     IGKEGPRYAVEEMTELKTVVIHGLS"
     gene            4282..6180
                     /gene="iolD"
                     /locus_tag="VF752_17545"
     CDS             4282..6180
                     /gene="iolD"
                     /locus_tag="VF752_17545"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX8857403.1"
                     /translation="MSTIRLTTAQAVVRYLSAQYSERDGERRRLIPGMFGIFGHGNVA
                     AVGQALEEHGGALTYLQPRNEQAMVHTASAFAKASRRLATLACTSSIGPGATNMVTGA
                     ALATINRLPVLLFPGDVYATRRQGPVLQQLEHPLEGDVSVNDAFRPVSRFFDRIARPE
                     QLLTALPEAMRVLTSPEEAGAVVISLHQDIQSEAYDFPERFFSERTWTVRRPPPEQGS
                     IDAVADLLAGAERPLIIAGGGVIYSEAESELEALAERHGIPVAETFGGKGAVRRPAWW
                     GLGGLGLEGNPACNQVAAEADVIVCVGTRLTDFATASQSLFQDPDVRFAAINVNDRDA
                     HKQNGVPVVADAREALRALGEALVARGVEPSDEYRSTIAERRDRWEQERDEALHADHE
                     QGMTQGRLIGVMQDSARAGDTIIAAAGGPPGDLLKVWDATEGRNCHLEFGYSCMGYEL
                     PAALGVRLAQPEGEVISFIGDGTFLMNPTEIVTAMQEGLKITLVISENHGFQVIRRLQ
                     MLRAGREFGNEFRERDGDGGANGALSGGRLAGDYLALDLAKVAEGLGAQAFRVSTPEE
                     TREALARAREAQGPVVIVAETVPHADLPGAGVWWDVAPSEVSGDEVVRKLRAEYEADR
                     ERLQRYYS"
     gene            6192..6932
                     /locus_tag="VF752_17550"
     CDS             6192..6932
                     /locus_tag="VF752_17550"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX8857404.1"
                     /translation="MKERLAAGETLVGAFANLGSPLAVEAMGVAGLDWVLVDLEHGGG
                     HEAALVGQLHAAAASGMHALVRVEAAERARAGRALDLGAEGVMFPRLDTVDEARAAIR
                     LLRYQPDGDRGVATYNRACGFGTRADAIDSVAERILGAVQVESPAAVDAVEEIAQLDG
                     VDVLFIGPGDLSHAMGIRGQIDHPDFRGAVERVVAAAESAGKGAGILVGSAAGLPGAI
                     EDGFRMIGVGSDSTLLAQAVGAVMAAAR"
     gene            6929..7864
                     /locus_tag="VF752_17555"
     CDS             6929..7864
                     /locus_tag="VF752_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8857405.1"
                     /translation="MTVAPAIVAAGPLADPAPEILGRFGPVVVAERDTAEALRPLLSE
                     AVGLVARGPTVVDAELLDAAPRLRVIGRSGVGTDRVDLEAATARGIPVVITPGANADA
                     VAEGAIALLLALIKRLPALDSAVRDGRWSERDSLGPGDVAGTTLAVVGYGAIGRRVAE
                     LARAIGMEVLVCDPLVESSVSLDEAVARADHLTLHVPLTGSTRGLVSRELLSRARPGL
                     RIVNMSRGGVAPLDVLDEGLRSGALGGVGLDVFDPEPPDPSHPFFANDGVVCSPHALA
                     LTPAAKRAVFTAMSEGMAAVLDGGRAAHVANPAVY"
     gene            8390..8692
                     /locus_tag="VF752_17560"
     CDS             8390..8692
                     /locus_tag="VF752_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857406.1"
                     /translation="MKRNTATTTARRKTTGAALTGASATGAMAVGARVFGPSAMGPLA
                     IAVSAIGALAIGRLAVANAVIRKLQAEEIEIGSLKVRELEVAGQRWPSATPAQAQT"
     gene            complement(8719..10380)
                     /locus_tag="VF752_17565"
     CDS             complement(8719..10380)
                     /locus_tag="VF752_17565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8857407.1"
                     /translation="MRTRWLVLALAAGLALADGSIVTLALPQILRELDTTVEGVAAVI
                     GVYTLVIAVALLPLERAARTWSLRAVGAGGFALMAIASLACAAADDLTALLVARGAQA
                     LGGAAGLVAAFALIGGDEARRRRTWVAAAVVGTAVGPALGGALTQVFSWQAIFVFQAP
                     LAAAAAVAAWTGPAHVAEPTSAPAHPKRFTLRPALGLALVSAALSAVLFLLVLLLVAG
                     WSISPLGAAAAVSVIPLGALAGTRVHGDPHVRAAAGSALLGGGVLALAWLPTASVAWT
                     LAPEVLAGFGMGLALPALAGGLLPERTTLDVARTLALRHAGIAAALALLAPIVAHQLT
                     VSTEHAREQGVALLLDAPLPLQDKIGLAPELLAGVDTQDPRDGLNRAVELQRGRFQGE
                     ERATYDRLGTRLDETLTSAVGDAFQPAFLITGGLALLAGVILTPWGQARRAAPALAGA
                     AVIAVLSPAAYAAADAAFGPEPVAILDPCTAKRKPPSTGGITGFVQDRALQLLDKAAC
                     RLGSSREELVLALSNETDAKRFENEHGVDPRSILGLLEGLFRGGP"
     gene            10472..10741
                     /locus_tag="VF752_17570"
     CDS             10472..10741
                     /locus_tag="VF752_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857408.1"
                     /translation="MSAFIITRIQVGDYDAWRPMFDQDRPRAREKAKSQRVFRGVDDP
                     GQVFIVLDFDSVEDAQESRERLLSSGVLDRFDDKHGPNVVAEVGA"
     gene            10854..11555
                     /locus_tag="VF752_17575"
     CDS             10854..11555
                     /locus_tag="VF752_17575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8857409.1"
                     /translation="MLPALKRYHDVLAPTFAGHAGGPPIDGEIADSFLADAVECAMDE
                     AGFDAAHIVGSSLGGWLALQLAARGRARTVVAFAPAGGWAEDDGSFRRTLGLQSAMVQ
                     YARSAAPQATSLLGTPEGRRRATQLITTNFEHIPADLLAHQMLGVAGCTTAGPLIEHA
                     LGQQWALDAEKITCPVRIAWGTQDALLPLDWAATRFRYEWLPSADWIELDGVGHCPQL
                     DIPLETAELIRGFTR"
     gene            complement(11552..11995)
                     /locus_tag="VF752_17580"
     CDS             complement(11552..11995)
                     /locus_tag="VF752_17580"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8857410.1"
                     /translation="MADSAVDVARESIECFNSGDFDRMRSLLADDSYEDEYATQRHLD
                     GADAQVDAARGWKQAFPDGRGTVRGACVDGNTVTLEITWSGTQSGPMRTPDGREMPAS
                     NRSVTVDACQVMEIEDGKVRATRHYFDLMTLLQQLGVMERAGAAV"
     gene            complement(12087..13436)
                     /locus_tag="VF752_17585"
     CDS             complement(12087..13436)
                     /locus_tag="VF752_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8857411.1"
                     /translation="MLNRSTTPDIRSVAREHYIWPQLPRDEVDAGVPQLLGDGEGVRV
                     RTVEGDEYLDLMSTVSRASSLGYGEERIARAVYEQLRRLHYGGTAHSQADVTIELAER
                     IARLAPGDLTACVFVGSGSEANEVAFKLARFYHHARGTKPRAYKVIARWNDYHGAAGG
                     ATAASDWLGVRRPMEPGVPGFSRIPSPISYRCPFGYDHQNCNEECGMRCADYLEQHIL
                     HEGPELVSAFILEPIAQADGVQIPPAGYLQRVREICAKYDVVFIADEVITGFGRTGEW
                     FSVDHWDVKPDIMTMAKALTAGYIPLGATIATREIRDALDGYPDIHTYGGHPAAAAAA
                     LTAIDIYADDGLLARAAQEGAYALDTLRGLEDLDAVGEVRGLGLWAAVDFTADPTTRE
                     PLPAETLKAIVKRTRELGVLVSNNGTAIEVAPPLIISREDLDEGLARFTQAVREIVG"
     gene            13553..14617
                     /locus_tag="VF752_17590"
     CDS             13553..14617
                     /locus_tag="VF752_17590"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX8857412.1"
                     /translation="MPLPVRPPLAPMLARLTRSLPREGYLYEPKWDGFRSISFRDRGE
                     IDMRSRHDRPLARYFPELVDALRAVPSESFVIDGEIVVVTERGFDFPATMARLHPAAS
                     RVERLARETPAQLIAFDLVSEGSDDLRETRFEERRVRLERLLSDPPDGLRLTPITDDA
                     EVAQDWLSRFEGGGIDGVMAKRRDLHYQPGARAMTKVKNERRADCVVAGFRVYVDAED
                     EVASLLLGLYDDRRELEHVGVASSMRRELRLRLFDELRPLIVPLQGHPWEHGYLIGGG
                     ALGRLPGSAGRWTPDMEHDWIPVRPERVAEVAYEQLDDFRFRHPARFRRWRPDRDPLS
                     CSIEQLRATPPPLITALEGR"
     gene            14614..15504
                     /gene="ligD"
                     /locus_tag="VF752_17595"
     CDS             14614..15504
                     /gene="ligD"
                     /locus_tag="VF752_17595"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8857413.1"
                     /translation="MTTTIGGRDLRLTNVDRVLWPEAGFTKGAMIDFYAAVADVLVPH
                     LARRPLTLGRWPSGVGHRGFAQNECRGAPEWMETAPLVLASGEVRNYCVVNDAASLVW
                     VANLVAIELHPYLAPVNAPDRPIAVAFDLDPYPPRGLLDACALALRLRELLADRGLEA
                     FPKTSGGTGMHIFVPLDGETGYDESRGFARTLAARLESETPDLVSTSTRPGDRRGRLF
                     VDWMQNSPRRSTVAPYSLRATDVPGVSTPVRWDEIEDALQDRTPTALVFGPDDVRERV
                     RRDGDLWRPMLELRQELPQG"
     gene            complement(15501..16835)
                     /locus_tag="VF752_17600"
     CDS             complement(15501..16835)
                     /locus_tag="VF752_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857414.1"
                     /translation="MSADYADLFCELFDRLSPAEFHANVEGWLADPGLMAGASEVERS
                     ALAGTLSATAAAEGSPFALLLGLDAALAHANPALGGVAPRAVAEYALCYAETGRLNSG
                     TLPGALLPRFARPGRRGQLPNDVADAFGATVRVGAAEWDDCDHRSLPAHARLTPADRE
                     GGLRIATAPMIREPAELSWRVLERGDMRFYRIHPCGSEATRRRIEQVIAAWDEHEVAI
                     GVVPELCLSADLLDEWRRALRGRERAASSSLRLVLAGTGDVEGASPPANTAVLLDALT
                     GETLASQRKLHPFNFSPSDLELWQLGDRLSAPIDEDLSRGERVCVIEAGGARIAMLVC
                     EDLARLHMCAGALHAHGVSLILVPVFARPTKDRRWERSRAEVYSDAIGSTVVVANSLV
                     MASILGSPSPVGTAIAVAPGEAAVGHASEAEDVVVFTLDGQAPRVTHERVPA"
     gene            16894..18072
                     /locus_tag="VF752_17605"
     CDS             16894..18072
                     /locus_tag="VF752_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8857415.1"
                     /translation="MPTVDQPREPDAPNSRTDAIVRRLDELTPDGPPSEGRLEALALG
                     LVDAVLDADEATLRAMLASLRAARSRMLEGDGPDRERLLGWLEAMTAATAGALDRVAP
                     AAPSGAVAPRTRAHDFLKALAGSRPVGSAELRSLLGTDETQVSRTGRGLLEAGLVSRA
                     KAGRQVFWRLTPRGRQALEEVDALEPAPGSPEFFMDAIRRGYEGRARPEPDPTRRRII
                     ESAMALHREHGVGKTTVSDIADQVGVDPATVESYFPTRDDLVLSCGQHILETLRLPPP
                     ERALEMFAGADAPHERARRMVETLFAAYERQGTSVANVEAERAALPLVANSLGQVDAC
                     VDALVREAVRGDPALLDPVRNVAGVRAWQELRKTVSHEEAVDEATAKVEAELSSAGRS
                     "
     gene            complement(18050..19195)
                     /gene="hutI"
                     /locus_tag="VF752_17610"
     CDS             complement(18050..19195)
                     /gene="hutI"
                     /locus_tag="VF752_17610"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX8857416.1"
                     /translation="MSTLAIDNIGLLVTNDPELRSVRDAALVLDGDRVAAIEPEGAAA
                     DERIDAAGRCVIPGFVDSHTHLVFAGDRAEEFAARMAGARYAAGGIRTTVEATRAASS
                     EELRALAEQRRQEALRAGITHLEIKSGYALDVDGEARLCSIASELSDDVTFLGAHVVP
                     AEYEGRSDDYVDLVRGEMLAACAPHARWIDVFCEEGAFDADQSRAILEAGRDAGLGLR
                     VHANQLGEGPGVQLAVEMGAASADHCTYLTDADVEALAASSTVATFLPATDFSTREPY
                     PDARRVIDAGATVAIATNCNPGSSYTTSMSFCIALAVREMHMTVEEAIEAATLGGATA
                     LGRDDLGHLRPGARADAVILEAPSPAHLVYRPGVPLIATTISSGRPS"
     gene            complement(19192..20520)
                     /locus_tag="VF752_17615"
     CDS             complement(19192..20520)
                     /locus_tag="VF752_17615"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX8857417.1"
                     /translation="MSYWCELAWLGGDRPEEGVVIDVSGDRIASIMTGVESPPGDAEH
                     LRGLTLPGLANAHSHAFHRALRGRAERGRGSFWTWREQMYELAARLDLDNYFDLARAT
                     FAEMALAGVTVVGEFHYLHHRAGGEPYDDPNAMGHVILQAAAEAGVRVTLLDACYLLG
                     GIGQEPDGVQERFSDGSVEAWRRRVEAIPQSEAAKVGAAIHSVRAVDPDSARVVATWA
                     AEHSRPLHAHVSEQPSENEDSIEAYGVTPTELLDREGALGKRFTAVHATHVTEPDVRL
                     LGDRRACCCLCPTTERDLADGVGPARMLAELGASLSLGSDSHAVIDIFEEARAVELDE
                     RLVTGARGHHTAAELLRAATESGYRCLGWPDGGRIEEEALADLVTVGLDSVRLAGATP
                     AEALEAVVFAGAAADVRHVIMGGRRIVWDGAHLALDVANELRHSIAAVTA"
     gene            complement(20526..21104)
                     /gene="def"
                     /locus_tag="VF752_17620"
     CDS             complement(20526..21104)
                     /gene="def"
                     /locus_tag="VF752_17620"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8857418.1"
                     /translation="MAVREIATVGHPVLRERALEVTPEELRSPEVQRLIDDMIDTKRA
                     ANGAGIAANQVYETLRIAVVEVEPDNPRYPYKPPVPLTVLVNPAIEPIDDETAEINEG
                     CLSVPDLRGVVERHVNVRVRYLDRHGDSHDEVRRGLTAGTFQHELDHLDGILFLDRVR
                     DPRTFMTWEQFERFHRDEFAVRAAALVERVGS"
     gene            complement(21104..>22776)
                     /gene="hutU"
                     /locus_tag="VF752_17625"
     CDS             complement(21104..>22776)
                     /gene="hutU"
                     /locus_tag="VF752_17625"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX8857419.1"
                     /translation="RARMSEGARPVRAPRGSEVSCRGWQQEAALRMLMNNLDPEVAED
                     PDRLVVYGGTGRAARSWEAFDAIVRELRAPGDDETLLVQSGKPVGVFRTHEWSPRVLI
                     ANSNLVPDWATWEEFRRLEDLGLTMYGQMTAGSWIYIGSQGIVQGTYECFAEIARRRY
                     GGSLAGTITLTAGLGGMGGAQPLAVTMNEGVALCVEVDPHRIERRLETRYLDEQADGI
                     DDAVARVRRAKEERRPLSVGLLGNAAEVFPALLDQGFEADVVTDQTSAHDPLSGYVPA
                     LMSYDEALALRTSDADEYVRRARRSMAAHCAAMVGYLDRGAEVFDYGNSLRAEAKLGG
                     FERAFAYPGFVPAYIRPQFCEGRGPFRWVALSGDPADIAATDRAVLAEFPDDERLARW
                     IGHAGEKIAFQGLPARICWLGYGERARLGLRFNEMVRSGELSAPIVIGRDHLDSGSVA
                     SPYRETEDMADGSDAIADWPLLNALVNTASGASWVSVHHGGGVGIGRSIHAGMVCVAD
                     GSEAAAAKLERVLTSDPGTGVMRHADAGYERAGEVARERGVRIPMQDG"
BASE COUNT         3278 a         8039 c         8117 g         3342 t
ORIGIN      
        1 cgcaggcggc gaaggacttc cgctggcttc cggcgatcgc ggacgcggtg aacaactcgg
       61 tgccgaagcc gaagacgccc gatgagccga agttcgagga catcctcggg acagagctca
      121 acaaggcgct ggtcgaggcg attcagaaga agagcggcta ccaggcgatc gcgcagaagc
      181 acctgaccac ggccgcgaat gagatgacga agttcatcaa gcagcagggc ggctacgtct
      241 aggccggacg gggcgagggt cagtggcaac tccctccgta gcagtgcccc gccgcgggcg
      301 ccggcgcagc gatttctggg ctcgggtact ggcgagcccc tggctgctcc tgacgcccgc
      361 ggcactggcc ctcgccgccg tcgccctcta tccgctggtc tacgggatcc gggccagctt
      421 ccacaagtac cgctacggcc gcgacgtcgg gtcggccggc ttcgagaact acaaggacgt
      481 cttccaggac cacaccttct ggacggcgat ggggacgacg gcgaagtacg tcgccctggc
      541 cgtctcggtc gagacgctgc tgggcctcgg gctcgcgctg ctcgtgtcga accagcttcg
      601 cttcagccgc ttcttccggg tcaccctgat cctgccgatg acgatcgcgc cggtggtcgt
      661 cggcgtgatc tggcgcctca tgtacgcgtc ggacatcggc gtggtgaacc cgctcttcaa
      721 cgccttcggc ctttccgacc cgaacgtgct ggcgaacccc ggcagcgcgt tcctaggggt
      781 ggtggtcgtc gacatctggg agtggacgcc gctgctcttc ctgatcatcc tcgcgggcct
      841 ccagtcgctt ccgcaggagc cgctcgaggc cgcgcgcgtg gatggcgcgg gacctgtgcg
      901 cgtgttcttc gaccacacgc tgccgctgct gcggccggtg ctgatggtgg ccgtggtgct
      961 gcgcctgatc gacgcgatcg ggaccttcga ccagatcttc gtgctcacca agggcgggcc
     1021 cggcacagcc acgcagctga tcagcaccta ctcgtacaac accgcattcc tgttcacgca
     1081 gtacgggcgc gcgatggcga tggtcgtctc gctgctcgtg ctggtgctga tcctgatggt
     1141 cgtcgcgatc cgttacatgc gcagcgccgc gatgaggacc gcgtcatgac gcgcgagcgc
     1201 ggcatcagct acgcgcgcgt cgccaccctg ctgctggcga tcgtggtggc gctgttcccg
     1261 ttctggtgga tgctcgtcac gtccttcaag cagcccgtgg acatcttcaa gggcgtgtcg
     1321 ctcgtgccgc agcacccgac cggcgacaac tacaaccgcc tgttcagcaa ctaccacatg
     1381 ggcgacttcc tggtgaacag cctcatcgtg gtgacggtcg cggtggcggt gagcctggtg
     1441 ctggggacgc tcgccgccta cgccctggcg cgctaccggc tgcgcttcca cctgaacgag
     1501 tccgcgctcg tggtgtcgct actggtccgc acgctgcccg ccatcctgct cgtgattccc
     1561 ctctacctca cgctcagcaa ggcggggctg ctgaacacac ggctcgggct gatcatcatc
     1621 tacacgggcc tgaacaccag cttcgtgatc tggatgatgc agagcttcct cgaggagatc
     1681 ccgaaggaaa tcgaggaggc ggcgatggtg gacggcgact cgcgcctgcg ggcgctccgg
     1741 cgcgtggtgc tgccgctggc aggacccggc ctcgctgcca cggcgatctt cagcgtgatc
     1801 gtgacctaca acgacttcct gctcgcgctc accgtcacgt ccacgccgag tgcgcagacc
     1861 gtgccggtcg gggtctccac gctcatcggc aagatccaga tcgagtgggg cccgatggcc
     1921 gccgccggcg tggtgggcgc gctgccgatc atgatcttcg cgatcctcgt gcagcgccac
     1981 ttcgtgcgcg ggctgacgct gggcgcggtg aagtgagcgc ggaggcaccg gtgctcgccg
     2041 cctgcgaatg gatcttcgcg ggccaggaag tgcctgcgct gctgcggcgg ctgtccgcgg
     2101 cgggctgcaa cgggatcgag gtctcgggcg agccggcgcg tcccgatcgg gcggagctgg
     2161 cggcggcgct cgaggaggcc ggcgtgcagg cgaccggcat caccgcgatc tgcccggcgc
     2221 cgaccgacga gcgggatctc tcgcatccgg atgcttccgc gcggcgtcgc gcggtcgagt
     2281 actaccgcgg ctgcgtggat ctcgcgcggg agctccgcgc gcccaccgtc ggcctgatcc
     2341 ccgcggcgat cggacgcgtg gacggctcgg tgaccgagtc gtggccgctc gccgtggagg
     2401 ccgcgcgtga ggtggccgac tacgcgggcg agcacggcgt cgtgctcggc gtcgaggcgg
     2461 tgaaccgcta cgagaccttc ctggtgaaca ccgcggatca ggcgttgcat ttcgcgcaag
     2521 agatcggtaa tgcaggcgtc gtgctcgacg ccttccacat gcagctcgag gaggaggaca
     2581 gcgtggccgc tgtcgtgcga gccgcgccaa ggctgcgggc acttcacctc gcggactcga
     2641 accgactcgg gcttggccac gggcagctcg atgccgcgcc tctagtcgcg gccgcgcgcg
     2701 gtgccggctt ttccggcccg ttcgtgttcg agttcaccgc gccgggaccg aacccgttca
     2761 accccgacaa gggagaggac gcgatgcgcg agctcgatgt ttacgcgcgc gagagcgtgg
     2821 cggcggtgga ggcagcgcta tgagtgagcg cgcgatgttc atcgggggcg agtgggcgac
     2881 cggcgacgcg cgccaggacg tgctgaatcc gtacagcggc gaggtgatcg ccaccgtccc
     2941 gcgtgcgggc gccgaagacg cggaccgcgc cgtggccgcg gcggtcgagg gcgcgcggca
     3001 gatgcgcgcg cttcccgcgc acgagcgcgc cgcgatcctc gagcgcgccg ccgacatcgc
     3061 cgacgagcgc gtcgacgaga tggcccgcac gatcgcgacc gagaccggca aggcgctgtc
     3121 cgaggcgcgc ggcgaagcgg gccgcgccgg agggatcctg cgggtgagcg cccacgaggg
     3181 cgctaccgcc gccggcgagg tgctgccgct ggacgcctcg cccggaggcg agggaaagct
     3241 gggcttcacg ctgcgccagc cgtgcggtgt ggtggttgcg atcacaccgt tcaactatcc
     3301 gctgctgctc gtgctgcaca aggtcgggcc ggcgctcgcg gccgggaacg cggtcgtgct
     3361 caagcctgcc gaggcaactc cgctcaccgc actgctgctc tgcgagatcc tgctcgaggc
     3421 cgggctgccg ccgctggcgc tgtcggtgat caccggctcg ggctcggagc tcgggccgct
     3481 gctgtgcgcc gactcccgcg tgcggaaaat ctcgttcacc gggtccacgc gcgtgggcga
     3541 ggagatcacg cgcgtggcgg gcgtgaaacg gctgtcgctc gagctcggcg cgacctgccc
     3601 gacgctggtg ctggccgacg ccgatatcga cgcagcggcc gcggcgatcg ccacaggcgg
     3661 ctacgtgaac gccggacagg tctgcatctc ggtccagcgc gtgatcgtgc agggcccggt
     3721 gcacggggac ttcctagacg ccctgctccc gcgcgtggag gcgatcgagg cgggcgaccc
     3781 gctcggcggc agcgcgacca tcggcagcct catcagcgag cgcgaggccg agcgcgtcga
     3841 gcggacgatc gccgaggcgg cggggaacgg cgcccgcgtg ctggtcggcg gcgaccggga
     3901 cggcgcggtg gtgtcgcccg cagtggtgga cggggtcgag ccgagcgcgc cgctctctcg
     3961 cgacgagctg ttcgggccgg ccgtggcggt cacgcgggtg gcggacgtgg acgaggccat
     4021 ccgcgtcgcc aacgactcga gctacggact gggcgccggg gtcttcacga acgacgtgac
     4081 ggccgcgatg cgcttcgtgc gcgaggtcga cgccggcgct ctgcacgtga actggacgcc
     4141 gctttggcgc gccgacccga tgccgtacgg tggcctgaag ggctcgggta tcggcaagga
     4201 agggccgcgc tacgcggtcg aggagatgac cgagttgaag accgtggtga tccacgggct
     4261 ttcgtgaggg gaggggatcc ggtgtcgacg attcgcttga ccaccgcgca ggcagtagtc
     4321 cgctacctgt cggcgcagta ctcagagcgc gacggcgagc gccgccgcct cattccaggc
     4381 atgttcggga tcttcggtca cggcaacgtg gcggcggtcg gtcaggcgct cgaggagcac
     4441 ggcggcgcgc tcacctacct gcagccgcga aacgagcagg cgatggtgca caccgcgagc
     4501 gcctttgcga aggcatcgcg ccgcctggcc acgctggcgt gcaccagttc catcgggccc
     4561 ggcgcgacga acatggtcac cggtgcggcc ctcgccacga tcaaccggct gcccgttctg
     4621 ctcttccccg gcgacgtcta cgccacccgg cgccagggcc cggtgctgca gcagctcgag
     4681 catcctctcg agggcgacgt gagcgtgaac gacgcgttcc ggcccgtgtc gcgcttcttc
     4741 gatcgcatcg cccggcccga acagctgctc accgcgctgc ccgaggcgat gcgggtgctc
     4801 acgagccccg aggaggcggg cgctgtcgtg atctcgctgc accaggacat tcagtcggag
     4861 gcctacgact tccccgagcg cttcttttct gagcgcacct ggaccgtgcg ccgcccgccg
     4921 ccggagcagg gttcgatcga cgccgtcgcg gatctgctcg ccggcgcaga gcgcccgctg
     4981 atcatcgcgg gcggcggcgt gatctacagc gaggcggagt cggagctcga ggcgctcgcg
     5041 gagcgccacg gaatccccgt ggcggagacc ttcggcggca agggcgccgt gcgccggcct
     5101 gcatggtggg gcctcggcgg gctcggcctc gagggcaacc cggcgtgcaa ccaggtcgcg
     5161 gcggaggccg atgtgatcgt gtgcgtgggc acccgcctga cggacttcgc gaccgcctcg
     5221 cagtcgctct tccaagaccc ggacgtgcgc ttcgccgcga tcaacgtgaa cgaccgcgac
     5281 gcccacaagc agaacggagt tcccgtcgtg gcggacgcac gcgaggcgct ccgcgctctg
     5341 ggtgaggcgc ttgtcgcgcg aggcgtggag ccctcggacg agtaccgctc gacgatcgcc
     5401 gagcggcggg accgctggga gcaggagcgc gacgaagccc tgcacgccga ccacgagcag
     5461 ggcatgaccc agggacgcct gatcggcgtg atgcaggact cggcgcgcgc gggcgacacg
     5521 atcatcgccg ccgcgggcgg cccgcccggc gacctgctga aggtctggga cgcgactgag
     5581 gggcggaact gccacctcga gttcggctac tcgtgcatgg gctacgagct gccggcggcg
     5641 ctgggcgtgc gtctcgccca gcctgagggc gaggtgatct cgttcatcgg cgacggcacg
     5701 ttcctgatga acccgaccga gatcgtgacc gcgatgcagg aggggctcaa gatcacgctc
     5761 gtgatctccg agaaccacgg cttccaggtg atccggcggc tgcagatgct gcgcgccggt
     5821 cgcgagttcg gcaacgagtt ccgagagcgc gacggcgatg gcggcgcgaa cggcgcgctg
     5881 tccggcgggc ggctcgccgg ggactatctc gcgctcgatc tggcgaaggt cgccgagggt
     5941 ctcggcgcgc aggcgttccg ggtctcgaca cccgaggaga cgcgcgaggc gctcgcgcgt
     6001 gcgcgcgagg cccaggggcc ggtagtgatc gtggcggaga ccgtgccaca tgccgacctt
     6061 cccggcgcgg gagtctggtg ggacgtggcg ccgtccgagg tgtcgggcga cgaggtggtt
     6121 cgcaagctgc gcgccgagta cgaggccgac cgcgagcgcc tgcagcgcta ctactcgtga
     6181 acgctccgac catgaaggag aggctcgcgg cgggcgagac cctcgttggc gcgttcgcga
     6241 acctcggtag cccgctcgcg gtcgaagcga tgggggtggc gggcctcgac tgggtgctgg
     6301 tcgatctcga gcacggcggc gggcacgagg ccgcgctggt cggccagctg cacgccgctg
     6361 ctgcgtcggg catgcatgcg ctcgtacgcg tcgaggcggc agagcgggca cgggctgggc
     6421 gcgcgctcga cctcggcgcg gagggcgtga tgttcccgcg cctcgacacg gtggacgagg
     6481 cgcgcgccgc gattcggctg ctgcgctatc agcccgacgg cgaccgcggc gtggccacct
     6541 acaaccgcgc gtgcggcttc ggcacgcgcg ccgacgcgat cgactcggtg gcagagcgca
     6601 tcctcggagc ggtgcaggtc gagtcgccgg cggccgtgga cgccgtcgag gagatagccc
     6661 agctcgacgg ggttgacgtg ctcttcatcg gacccggcga cctctctcat gcgatgggca
     6721 ttcgcgggca gatcgaccac ccggacttcc gcggggcggt cgagcgtgtc gtcgccgcgg
     6781 ccgagtccgc cggcaagggc gccggcatcc tcgtgggctc ggccgccgga cttccgggcg
     6841 cgatcgagga cggcttccgc atgatcggcg tcggctccga ctcgaccctg ctcgcgcagg
     6901 cggtcggggc ggtgatggcc gccgcgcggt gaccgtcgcg cccgcgatcg tcgcggccgg
     6961 tccactcgcc gatccggcgc cggagatcct cggccgcttc ggaccggtgg tcgtggccga
     7021 acgcgacacc gccgaggctc tgcgaccgct cctgtcggaa gcggtcggcc tagtggcgcg
     7081 cggaccgacg gttgtcgacg ccgagctgct cgacgcagct ccgcgcctgc gcgtgatcgg
     7141 gcgcagcggt gtgggcaccg accgcgtcga cctcgaggcc gccaccgcgc gcggaatacc
     7201 ggtcgtgatc acgcccggag ccaacgccga cgctgtcgcc gagggcgcga tcgccctgct
     7261 gcttgcgctg atcaagcggc tgccggcgct cgactcagcg gtgcgcgacg ggcgctggtc
     7321 ggagcgggat tcgctcgggc ctggcgacgt ggccgggacg accctggcgg tggtcggcta
     7381 cggggcgatc ggccgacgcg tggctgagct ggcgcgggcg atcggcatgg aggtgctggt
     7441 gtgcgatcca ctggtcgaga gctcggtctc gctcgatgag gcggtagcgc gtgcggatca
     7501 cctcacgctt catgtgccgc tcaccggctc aacgcgcggg ctggtgtcac gcgagctgct
     7561 gtcgcgcgcc cggcccgggc tgcggatcgt gaacatgagc cgcggcgggg tcgcgccgct
     7621 tgacgtgctc gacgagggcc tgcgctcggg cgctctcggc ggtgtcggcc tcgatgtctt
     7681 cgaccccgag cctccggacc cgtcgcaccc gttcttcgct aacgacggcg tcgtgtgttc
     7741 cccgcacgcg ctcgcgctca caccggctgc gaagcgcgcc gttttcaccg cgatgagcga
     7801 gggcatggcc gcggttttgg acggcggccg cgcggcgcac gtggctaacc cggccgtcta
     7861 ttagtcgaca cagggactcg ggcctaaccc ggacgttcct cgggtaaggc aaacgagggg
     7921 cgatggctgg atcagacttt cacgtcgacg caagcgaact cgttgccggg gtcacgcgtg
     7981 taaggcttgc tggtgagatc gacctctcgg tcgcaccgct tctccgacaa tcgctcaccg
     8041 atctcgtccg gcagggacac aacaagctgc tcatcgacct caccggcgtc accttcattg
     8101 actcgagcgc tatcggcgtc ctactgagca ctgtacggcg acttcggcgc cgacgcggga
     8161 gcctcgcagt cctctgtccc aacccccgac tacggcgcct attcgagcta gtcgggcaca
     8221 acctcatttt cccggtcgac gagacactcg agcaagcgct ccgccatctc cacgccaccg
     8281 gccgtccgac ggctccatca cacgcgctcc gctgaccgct tgctgcggca cttgcaggtc
     8341 cgctccaccg cctgtaaacc gagaggctcg cccacatagg aggtcgagca tgaagcgcaa
     8401 taccgctacc accaccgccc gccgcaagac aaccggcgca gcactcaccg gtgcgagcgc
     8461 caccggcgcg atggccgtcg gagctcgcgt gttcggacca tcggccatgg gcccgctcgc
     8521 gatcgccgtc tccgccatcg gcgcgttggc aatcgggcga ctcgctgtgg ccaacgcggt
     8581 aattcgcaaa ctccaagccg aggagatcga gatcggctca ctcaaggtgc gtgagctgga
     8641 agtcgccggc cagcgctggc ccagcgccac ccccgcgcaa gcgcagacat agttacgcgc
     8701 gcgacgtagc gttcgcgcct acggcccgcc gcggaacagg ccctcgagca gccccaagat
     8761 cgaccgcggg tcgacgccgt gctcgttctc aaagcgcttt gcatcggtct cgttcgagag
     8821 cgcgagcacc agttcctcgc gtgatgagcc cagtcggcag gccgccttgt cgaggagctg
     8881 gagcgcgcgg tcctggacaa agccggtgat gccgccggtg gacggcggtt ttcgcttcgc
     8941 cgtgcacgga tcgaggatcg cgaccggctc gggcccgaat gcggcatcag cggcggcgta
     9001 ggccgcgggt gacagcaccg cgatcaccgc tgcaccggcg agcgccggcg ccgctcttcg
     9061 cgcttgtccc cagggggtca ggatcacgcc cgcaagaagc gcaagcccac ccgttatcag
     9121 gaacgctggc tggaaggcgt cgccgacggc gctcgtgagc gtctcgtcga ggcgggtacc
     9181 aagccggtcg taggtggcgc gctcttcgcc ctggaagcgc ccgcgctgca actcgaccgc
     9241 acggttcagc ccatcgcgcg ggtcttgtgt gtcgactccc gcgagcagct caggcgccag
     9301 cccgatcttg tcctgaagcg ggagcggggc gtcgagcagg agcgcgacgc cctgctctcg
     9361 ggcatgctcg gtcgagacgg tcagctggtg agcgacgatc ggcgcaagca gcgcaagcgc
     9421 cgccgcgatg ccggcgtggc gcagggcgag cgtgcgggcg acatcgagcg tggtgcgctc
     9481 gggcagcagg ccgccggcca gcgcgggcag cgcgagaccc atgccgaaac cagcgagcac
     9541 ctcgggcgcg agcgtccagg caacgctcgc cgtgggcaac caggcgaggg ccagcacgcc
     9601 gccgccgagc agtgcgctcc cggccgcggc acgcacatgg gggtccccgt gcacgcgtgt
     9661 tccggcgagg gcgccgaggg ggatgacgct gactgcggcc gccgcgccaa gcggcgagat
     9721 gctccagccg gcgacgagga gcagcaccag gaggaacagg acggcgctga gcgcagccga
     9781 gacgagtgcc aggccgaggg cggggcggag cgtgaagcgc ttcggatgtg ccggcgccga
     9841 cgtgggctcc gcgacgtgag ccgggcctgt ccatgctgcg accgcggccg ccgcggccag
     9901 cggcgcttgg aacacgaaga tcgcctgcca cgagaacacc tgcgtgagcg cgccgccgag
     9961 cgccggaccc acagccgtac cgaccaccgc cgcggcgacc catgtgcgcc ggcgccgcgc
    10021 ttcatcgccg ccgatgagtg cgaacgccgc cacgagaccg gcggcaccac cgagtgcctg
    10081 cgcgccacgc gcgacgagga gggccgtgag gtcgtcggcg gccgcacagg ccagcgaggc
    10141 aatcgccatc aacgcaaagc cgccggcgcc aaccgcgcgc aacgaccacg tgcgcgcggc
    10201 gcgctctagc gggagcagcg ccacggcgat caccagcgtg tagacgccga tgacagctgc
    10261 cacgccctcg accgtggtgt cgagctcccg caggatctgc ggaagcgcca gcgtgacgat
    10321 cgatccgtca gccagcgcca gaccggcggc gagcgccaag acgagccatc gagtgcgcat
    10381 cactcaaagt ctgaacccgg acgggccggg cgccgcacgt cgcgaccccg ctgtatacaa
    10441 agcgccagcg actctcgacc gaaggggcgg aatgtctgcg ttcatcatca cccgcatcca
    10501 agtgggcgac tacgacgcct ggaggccgat gttcgaccag gaccggccgc gcgcacgcga
    10561 gaaggccaag agccagcgag tgttccgcgg cgtcgacgat ccgggccagg tcttcatcgt
    10621 cctcgacttc gactccgtgg aagacgctca ggagtcacgc gagcggctgc tgtcgtcggg
    10681 cgtgctcgac cgcttcgacg acaagcacgg cccgaacgtc gtggcggagg tgggtgcctg
    10741 atgcactact cggagacgtg aactccacct tcacgcgcgc gcaccgcggc ggctccgggc
    10801 cgccgctcgt gttgctgcac ggattcatcg acacgtggcg cacttgagag ctcgtgctgc
    10861 cggcgctcaa gcgataccac gacgtgttgg cgccgacctt cgcggggcac gccggcggac
    10921 ctccgatcga cggcgagatc gccgactcct tcttggcgga cgctgtcgag tgcgcgatgg
    10981 acgaggccgg cttcgacgcg gcgcacatcg tcggcagctc cctcggcggc tggctcgcgc
    11041 tgcagctcgc cgcgcgcggg agagcgcgga cggtggtggc gttcgccccg gccggcggct
    11101 gggctgaaga cgacggttcg ttcaggcgga cgctcggact gcagtcggcg atggtgcagt
    11161 acgcgaggtc ggccgcgccg caggccacct cgctgctggg cacgccggaa ggacggcgtc
    11221 gcgcgacgca gctgatcacc acgaacttcg agcacatccc cgcagacctg ctcgcccatc
    11281 aaatgctcgg tgtggccggc tgcaccacgg caggcccgct catcgagcac gcgctcggcc
    11341 agcaatgggc tctcgatgcc gagaagatca cgtgcccggt gcggatcgcc tggggcaccc
    11401 aggacgcgct actcccgctg gactgggcgg ccacccgctt ccgctacgaa tggctcccga
    11461 gcgccgactg gatcgagctc gacggtgtgg gccactgccc acaactcgac atcccgctcg
    11521 agaccgccga gctgatccgg ggctttacgc gctagacggc tgcgccggcg cgctccatca
    11581 cgccgagctg ttgcaggagc gtcatgaggt cgaagtagtg gcgggtcgcc ctgaccttcc
    11641 cgtcctcgat ctccatgacc tggcaggcgt ccactgtcac gctgcggttc gacgccggca
    11701 tctcgcggcc gtcgggcgtc cgcatcgggc cgctctgcgt gcccgaccag gtgatctcga
    11761 gcgtgaccgt gtttccgtcc acgcacgccc ctcgtaccgt gccgcgtcca tccgggaacg
    11821 cttgcttcca gccgcgtgca gcgtcgacct gagcgtcggc tccgtcgaga tggcgctgag
    11881 tggcgtactc gtcctcgtag gagtcgtccg ccagaagcga gcgcatgcgg tcgaagtcac
    11941 cgctgttgaa gcactcgatg ctctcgcggg ccacgtctac tgcgctgtcg gccattgagg
    12001 cctcccttcg gtggaccaga ggcgccccct cacggcgagc gtacgcccgg ggccccgagc
    12061 aagtcaacgg cccgcggaag cgcgctctac ccgacgatct cgcgaacggc ctgcgtgaag
    12121 cgagcgaggc cctcgtcgag atcctcgcgg ctgatgatca gcggcggcgc gacctcgatc
    12181 gccgtgccgt tgttgctcac gagcacgccg agctcgcgcg tgcgcttcac gatcgccttg
    12241 agggtctcgg cggggagcgg ctcgcgcgtc gtcggatcgg cggtgaagtc gaccgcggcc
    12301 cacagcccca gcccccgcac ctctccgacg gcgtccaggt cctcgagccc gcgcagcgtg
    12361 tcgagcgcgt acgcgccctc ctgcgcggcg cgcgcgagca gaccgtcgtc cgcgtagatg
    12421 tcgatcgcgg tgagcgccgc cgcggccgcc gccgggtggc cgccgtaggt gtggatatcc
    12481 gggtaaccgt cgagcgcgtc gcggatctcg cgggtggcga tcgtcgcgcc gagcggtatg
    12541 tagccggcag tgagcgcctt cgccatggtc atgatgtccg gcttgacgtc ccagtggtcc
    12601 acgctgaacc actccccggt gcggccgaag ccggtgatca cctcgtccgc gatgaagacg
    12661 acgtcgtact tcgcgcagat ctcgcgaacg cgctgcaggt agccggcggg cgggatctgc
    12721 acgccgtcag cctgcgcgat cggctcgagg atgaacgccg acacgagttc cgggccctcg
    12781 tgcaggatgt gctgctcgag gtagtccgcg cacctcatgc cgcattcctc gttgcagttc
    12841 tgatggtcgt agccgaacgg gcagcggtat gagatcggcg acgggatccg cgagaagccc
    12901 ggcacacccg gctccatggg gcggcggacg ccgagccagt cgctcgctgc ggtcgccccg
    12961 cctgcggcgc cgtggtagtc gttccagcgc gcgatcacct tgtaggcgcg cggcttcgtg
    13021 ccacgcgcgt gatggtagaa gcgcgcgagc ttgaaggcca cttcgttcgc ctccgagccg
    13081 ctgccgacga acacgcaggc agtgaggtcg cccggggcga ggcgggcgat ccgctcagcc
    13141 agctcgatcg ttacgtcggc ctgtgagtgc gcggtgcccc cgtagtggag gcggcgaagc
    13201 tgctcgtaga ccgcccgggc gatgcgctcc tcgccgtagc cgagcgacga cgcgcgcgag
    13261 accgtgctca tgaggtcgag gtactcgtcg ccctcgaccg tccgcacccg cacgccctca
    13321 ccgtcgccca gcagctgagg cacgccggcg tccacctcat cgcgcgggag ctgcggccag
    13381 atgtagtgct ccctcgcgac ggagcgaata tcgggggtag tagaccggtt cagcacaaca
    13441 acactttcat gccataaata gggctgtcaa ggggagtgga acgtttttgc aatgtgagcc
    13501 gctctccgcg tcagagtttc ggctccggct ccgcgtggca acccgctgag cgatgcctct
    13561 cccggttcga ccgcccctcg ctccgatgct cgctcgcctg acccgctcgc tgccgcgcga
    13621 gggctacctg tacgagccga agtgggacgg cttccggtct atctcgtttc gcgaccgggg
    13681 agagatcgac atgcgcagcc gccacgaccg gccgctcgcc cgctacttcc cggagctcgt
    13741 cgacgcgctt cgtgccgtgc cgtccgagag cttcgtgatc gacggcgaga tcgtggtcgt
    13801 gaccgagcgc ggcttcgact tcccggccac gatggcgcgg ctgcacccgg ctgcctcgcg
    13861 cgtggagcgt ctcgctcgtg agacgcccgc ccagctcatc gcgttcgacc tcgtgagcga
    13921 gggttcggac gaccttcgcg aaacgcgctt cgaggagaga cgcgtgcggc tcgagcggct
    13981 cctgtccgat ccacccgacg gccttcgcct cacgccgatc accgacgacg ccgaagtggc
    14041 gcaggactgg ctctcgcgat ttgaaggagg agggatcgac ggcgtgatgg ccaagcgccg
    14101 cgacctgcac taccaaccgg gagctcgtgc gatgacgaag gtgaagaatg agaggagagc
    14161 ggactgcgtc gtggccggct tccgcgtgta cgtcgacgcg gaggacgagg tcgcctccct
    14221 gctgctcggc ctctacgacg atcgacgcga gctcgagcac gtgggcgtgg cgagctccat
    14281 gcggcgcgag ctgcggcttc gactattcga cgagctccgg ccgctgatcg ttccgctgca
    14341 gggacaccca tgggagcacg ggtacctcat aggcggaggc gcgctcgggc ggctgccggg
    14401 gtccgccggg cgctggacgc ccgacatgga gcacgactgg atcccagtga ggccggagcg
    14461 cgtggccgag gtcgcctacg agcagctcga cgatttccgc tttcgccacc cggcgcgctt
    14521 ccggcgctgg cggccggacc gcgacccgct ttcctgcagc atcgagcagc tccgcgcgac
    14581 gccgcctccg ctcatcactg cgctcgaggg ccgatgacga cgaccatcgg cggccgtgac
    14641 ttgcggctca ccaacgtgga tcgggtcctg tggccagagg ccggcttcac caagggcgcg
    14701 atgatcgact tctacgcggc ggtcgccgat gttctcgtcc cgcacctcgc acggcgcccg
    14761 ctgacgctcg gccgctggcc gtcgggcgtc gggcatcgag gcttcgcaca gaacgagtgc
    14821 cgtggcgctc cggagtggat ggagacggcg ccactcgtac tggcgagcgg cgaggtgcgg
    14881 aactactgcg tcgtgaacga cgcggcctca ctggtctggg tggcgaacct cgtagcgatc
    14941 gagctacatc cctacctcgc gccggtcaac gcgcccgatc gaccaatcgc cgtcgcattc
    15001 gatctcgatc cgtatccgcc gcgagggctg ctcgacgcgt gcgcgctggc tctgcgcctg
    15061 cgggagctac tcgccgacag agggctcgag gcattcccga agacttccgg cggcacgggc
    15121 atgcacatct tcgtgccgct cgacggagag accggctacg acgagagccg tgggttcgcg
    15181 cgcacactcg ccgcgcgcct cgagagcgag acgcctgacc tcgtcagcac gagcacccgc
    15241 ccgggcgacc gccgtgggcg gctgttcgtc gactggatgc agaactcgcc ccgccgctcg
    15301 accgtcgcgc cgtactcgct gcgcgccacg gacgtccccg gggtctcgac gcccgtgcgc
    15361 tgggatgaga tcgaggacgc actgcaagac cgcactccaa ctgccctcgt gttcggtccc
    15421 gacgacgtac gcgagcgagt cagacgcgac ggcgaccttt ggcggccgat gctcgagctg
    15481 cggcaggagc tgccgcaggg ttaagccggc actcgctcgt gcgtgacgcg cggcgcctgg
    15541 ccgtcgagcg tgaacaccac cacgtcctcg gcttcggacg cgtgtccgac cgcggcctcg
    15601 ccgggcgcga cggcgatcgc cgtgccgacc gggctcgggc tcccgaggat cgacgccatc
    15661 accaggctgt tcgcgaccac gaccgtcgag ccgatcgcgt cgctgtacac ctccgcgcgc
    15721 gatcgctccc agcgccggtc cttggtgggg cgcgcgaaca ccggcacgag gatcagcgac
    15781 acgccgtgcg cgtggagcgc gccggcgcac atgtgaagcc gggcaaggtc ctcgcacacg
    15841 agcatcgcga tgcgggcccc gccggcctcg atcacgcaga ccctctcgcc gcggctgagg
    15901 tcctcgtcga tcggcgcgct caggcggtcg ccgagctgcc acagttcgag gtcgctcgga
    15961 gagaagttga acggatgcag cttccgctgg ctcgcgagcg tctcgccggt gagcgcgtcc
    16021 agcaacaccg cggtgttggc agggggactt gcgccctcga cgtcgccggt gccggcgagc
    16081 acgagccgca ggctgctcga cgctgcgcgt tcacgtccgc ggagcgcccg gcgccactcg
    16141 tcgagcagat cggccgacag gcacagctcc ggtacgaccc caatcgcaac ctcgtgctca
    16201 tcccacgccg cgatcacctg ctcgatccgg cgccgcgtgg cctcactgcc gcacgggtgg
    16261 atccggtaga agcgcatgtc gccgcgctcc agcactcgcc acgagagctc cgcgggctcg
    16321 cgaatcattg gtgccgtggc gatacggagg ccgccctcgc gatcggccgg agtgaggcga
    16381 gcgtgggcgg gcagcgagcg gtgatcgcag tcatcccact ccgcggcgcc gacgcgcacg
    16441 gtcgcaccga atgcgtcggc gacgtcgttc gggagctggc cgcgacggcc cggccgggca
    16501 aagcgcggca gcagcgcacc gggcagcgtg cccgagttga gtcggccggt ctcggcgtaa
    16561 cacagcgcgt actcggcgac cgcgcgcggc gccactccac ccagcgccgg gttcgcgtgg
    16621 gcgagggcag cgtcgaggcc gaggaggagc gcgaacggtg agccttctgc ggcggctgtc
    16681 gcgctcagcg ttccggcgag cgcggagcgc tcgacttcac tcgcgccggc catcaggccc
    16741 ggatcggcga gccagccctc gacgttcgcg tggaactcag ccggcgagag gcggtcgaag
    16801 agctcgcaga agagatcggc gtagtcggcg ctcacttgac aaacacttga cacatccgga
    16861 taccagcgct accttcggtg agccgtggct gcgatgccca ccgtagacca accgcgggag
    16921 ccggatgccc cgaattcgcg cacggacgca atcgtgcgca ggctcgacga gctcacgccc
    16981 gacggccctc catccgaggg ccggcttgag gccctggcgc tcggcctggt cgatgccgtt
    17041 ctcgacgctg acgaggccac gctgcgcgcc atgctcgcct cgctccgcgc agcccgctcg
    17101 cgcatgctcg agggcgatgg ccccgatcgc gagcggttgc tcggctggct cgaggcgatg
    17161 acggctgcga ccgcgggagc gctcgaccga gtcgcgccgg ccgctccgag tggcgcggtc
    17221 gcgcctcgca cgcgcgcgca cgacttcctc aaggcgctcg cgggatcgcg gcccgtcggg
    17281 agcgccgagc tccgcagcct gctcggcact gatgagacgc aggtgagccg caccggacgc
    17341 gggctgctcg aagcgggcct ggtgagccgc gcgaaggcgg gccgccaggt gttctggcgg
    17401 ctcaccccgc gcgggcggca ggcgctcgag gaggtggatg cgctcgagcc cgcgcccggc
    17461 agccccgagt tcttcatgga cgcgatccgt cgtgggtacg agggccgcgc acggccggag
    17521 cccgacccga cccgtcgccg gatcatcgag agcgcgatgg cgcttcacag ggagcatgga
    17581 gtcgggaaga cgaccgtgtc ggacatcgcc gaccaggtgg gcgttgatcc ggcgaccgtc
    17641 gagtcctact tccccacccg ggacgacctc gtgctgagtt gcggccagca cattctcgaa
    17701 acactgcgcc tgcccccgcc cgagcgcgcc ctcgagatgt tcgccggggc cgacgcgcca
    17761 cacgagcgcg cgaggcgcat ggtcgagacg ctgtttgctg cctacgagcg gcagggcacg
    17821 tccgtcgcga acgtggaggc ggagcgtgcc gccctgccgc tcgtcgccaa ctctctcggc
    17881 caggtggacg cgtgcgttga cgcgctggtg cgcgaggcag tccgcggaga tcccgcgctc
    17941 ctcgacccgg ttcgaaacgt cgccggagtg cgcgcgtggc aggagctgag gaagacggtt
    18001 tcccacgagg aggcggtcga cgaggcgacg gccaaggtcg aggcggagct cagctcggcc
    18061 ggccggagct gatcgtcgtc gcgatcagcg gcacgcccgg gcgatagacc aggtgcgccg
    18121 gcgacggcgc ttcgagaatt accgcgtcgg cacgggcgcc cggtcgcagg tgcccgaggt
    18181 catcgcgccc cagcgccgtc gcgccgccga gcgtggcggc ctcgatcgcc tcctccaccg
    18241 tcatgtgcat ctcgcgcacc gcgagcgcga tgcagaagct catggaggtc gtgtagctcg
    18301 agccgggatt gcagttcgtg gcgatggcga cggtcgctcc cgcgtcgatc acccgccgcg
    18361 cgtcgggata gggctcccgc gttgagaagt cggtcgccgg gaggaaggtg gcgacggtcg
    18421 agctcgcggc gagcgcctcc acgtcggcgt cggtgaggta ggtgcagtgg tcggccgatg
    18481 ccgcgcccat ctccacggcg agctgcacac ccggtccctc gccgagctgg ttggcgtgta
    18541 cgcgcaggcc gaggcccgcg tcgcgcccgg cctcgaggat ggcgcgcgac tggtcggcgt
    18601 cgaacgcacc ctcttcgcag aacacgtcga tccagcgcgc gtgcggcgcg caggccgcga
    18661 gcatctcgcc gcggacgagg tcgacgtagt cgtctgagcg gccctcgtac tcggccggca
    18721 ccacgtgggc accgaggaac gtgacgtcgt cgctcagctc ggacgcgatc gagcaaagcc
    18781 gtgcctcgcc gtccacgtcg agcgcgtagc ccgacttgat ctcgaggtgg gtgatgcccg
    18841 cgcgcaatgc ttcctgccgg cgctgctccg cgagagcacg cagctcctcg ctggacgcgg
    18901 cccgcgtggc ctcgacggtc gtccggatgc cgcccgccgc gtagcgggcg cctgccatgc
    18961 gcgccgcgaa ctcctcggcg cggtcgcccg cgaacacgag gtgcgtgtgg ctgtccacga
    19021 agccggggat gacgcagcgc ccggcagcgt cgatgcgctc gtcggcggcc gcgccctcgg
    19081 gctcgatcgc cgccacgcgg tcgccgtcga gaacgagcgc cgcgtcgcgc accgagcgga
    19141 gctccgggtc gttggtgacc agcagcccga tgttgtcgat cgcgagcgtg ctcacgcggt
    19201 gaccgcggcg atcgagtgcc tcagctcgtt cgcgacgtcg agcgcgagat gcgcgccgtc
    19261 ccacacgatg cggcggccgc ccatgatcac gtggcgcacg tcagccgcgg cgccggcgaa
    19321 caccacggcc tcgagcgcct cggccggcgt tgcacccgcc aggcgcacgc tgtcgagtcc
    19381 gaccgtcacg aggtcggcga gcgcctcctc ctcgatccgg ccgccgtccg gccatccgag
    19441 gcagcggtag ccggactcgg tcgcggcccg caggagctca gcggcggtgt ggtggccgcg
    19501 cgcgccggtg acgaggcgct cgtcgagctc caccgcgcgg gcctcctcga agatgtcgat
    19561 gacggcgtgc gagtcgctgc cgagcgacag cgacgcgccg agctcggcga gcatgcgggc
    19621 ggggccgact ccgtcggcca ggtcacgctc ggtggtgggg cagaggcagc agcaggcgcg
    19681 ccggtcgccg agcagtcgca cgtccggctc ggtcacatga gttgcgtgaa ccgccgtaaa
    19741 gcgcttgccg agcgccccct cccggtcgag cagctctgtc ggcgtgacgc cgtatgcctc
    19801 gatcgagtcc tcgttctcgc tcggctgctc cgacacgtgc gcgtgcaagg gccgcgagtg
    19861 ctccgcggcc cacgtggcga cgacccgagc cgagtccggg tcgacggcgc gcacgctgtg
    19921 gatcgccgca cccaccttgg cggcctccga ctgcggaatc gcctccacac gccggcgcca
    19981 cgcttcgacg ctgccgtcgg agaagcgttc ctgcaccccg tctggctcct ggccgattcc
    20041 gccaagcagg taacaggcgt cgagcagcgt gacgcgaacg cccgcctcgg cggctgcctg
    20101 caggatcacg tggcccatcg cgttcgggtc gtcgtagggc tcgccgcccg cgcggtggtg
    20161 gaggtagtgg aactcaccga ccaccgtcac gccggcgagg gccatctccg cgaacgtggc
    20221 ccgcgcgaga tcgaagtagt tgtctaggtc gagcctggcg gcgagctcgt acatctgctc
    20281 gcgccaggtc cagaacgagc cgcggccccg ctcggcgcgc ccccggagcg cgcggtggaa
    20341 cgcgtgagaa tgcgcgttcg cgagccccgg aagcgtgagg ccgcgtaggt gctcggcgtc
    20401 gcccggcgga gactcgactc cggtcatgat cgacgcgatc cggtcgcccg acacgtcgat
    20461 cacaacgccc tcctcgggcc ggtcgccgcc gagccacgcg agctcgcacc agtaggacat
    20521 gaacgtcaag agcccacccg ctccacgagc gcggccgcac gcaccgcgaa ctcgtcgcga
    20581 tggaagcgct cgaactgctc ccaggtcatg aacgtgcgcg gatcgcgcac gcggtcgagg
    20641 aacaggatgc cgtcgaggtg gtcgagctcg tgctggaagg tcccggccgt gagcccgcgt
    20701 ctcacctcgt cgtgtgaatc gccgtgccgg tcgaggtagc gcacccgcac gttcacgtgc
    20761 cgctccacca caccgcgcag gtcgggcacc gacaggcagc cctcgttgat ctccgccgtt
    20821 tcgtcgtcga tcggctcgat cgccgggttc acgagcacgg tgagcggcac gggcggcttg
    20881 tacgggtagc gcgggttgtc cggctcaacc tccacgacgg cgatgcgcag cgtctcgtac
    20941 acctggttcg ccgcgatgcc ggcgccgttt gcggcgcgct tcgtgtcgat catgtcgtcg
    21001 atcaggcgct gaacctcggg cgagcgcagc tcctcgggcg tcacctcgag cgcacgttcg
    21061 cgcagcaccg gatggccgac ggtggcgatc tcgcggaccg ccatcagccg tcctgcatcg
    21121 ggatccgcac tccccgctcg cgggcgacct cgcccgcgcg ctcgtagccg gcatcggcgt
    21181 gacgcatgac gcctgtgccg ggatcgctcg tcagcacgcg ctcgagcttc gccgccgcag
    21241 cctcgctgcc gtccgccacg cacaccatgc ccgcgtgaat cgaccggccg atgcccacgc
    21301 ctcctccgtg gtggacgctc acccacgacg cgcccgacgc cgtgttcacg agcgcattca
    21361 ggagcggcca gtcggcgatg gcgtcggagc cgtccgccat gtcctcggtc tcgcggtagg
    21421 gcgacgcgac cgacccggag tcgaggtggt cgcgcccgat tacgatcggc gcgctcagct
    21481 cgccgcttcg aaccatctcg ttgaagcgca agccgaggcg ggcgcgctca ccgtagccga
    21541 gccagcagat gcgcgccggc agtccctgga acgcgatctt ctcaccggcg tgcccgatcc
    21601 agcgcgcgag ccgctcgtcg tcggggaatt ccgccagcac tgcccggtcg gtcgcggcga
    21661 tatccgcggg atcgcccgac agcgcgaccc agcggaacgg cccgcgcccc tcgcagaact
    21721 gggggcggat gtaggcgggc acgaagcccg ggtaggcgaa cgcgcgctcg aagccgccga
    21781 gcttcgcctc cgcgcgcagg ctgttcccgt agtcgaacac ctccgcgccg cggtcgaggt
    21841 agcccaccat cgccgcgcag tgcgcggcca tcgagcggcg cgcgcggcgg acgtactcgt
    21901 cggcgtcgct ggtgcggagc gcgagcgcct cgtcgtagct catgagcgcc ggcacgtagc
    21961 cgctcagtgg gtcgtgcgcg ctcgtctggt ccgtgaccac gtcggcctcg aagccctggt
    22021 cgagcagcgc ggggaacacc tcggcggcgt tgccgagcag gccgacgctc agcggccggc
    22081 gttcctcctt ggcccgccgc acccgcgcga cggcgtcgtc gatcccgtca gcctgctcgt
    22141 cgaggtagcg cgtctcgagc cggcgctcga tccggtgcgg gtcgacctcc acgcacagcg
    22201 ccacgccctc gttcatcgtc acggcgaggg gctgggcgcc gcccatgccg ccgagcccgg
    22261 cggtgagcgt gatcgttcct gcgagcgagc cgccgtagcg gcgccgcgcg atctccgcga
    22321 agcactcgta ggtgccctgc actatcccct ggctgccgat gtagatccag gagcccgcgg
    22381 tcatctgccc gtacatcgtc aggccgaggt cctcgaggcg gcggaactcc tcccacgtgg
    22441 cccaatcggg caccaggttc gagttggcga tcagtacgcg cggcgaccac tcgtgcgtgc
    22501 ggaacacgcc taccggcttg cccgactgca cgagtagggt ctcgtcgtca ccgggcgcgc
    22561 ggagctcgcg cacgatcgcg tcgaaggcct cccaggagcg cgccgccctg ccggtgccgc
    22621 cgtagacgac caggcggtcg gggtcctcgg ccacctcggg gtcgagattg ttcatcagca
    22681 tgcgcagcgc cgcttcctgc tgccagccgc ggcacgagac ctcgcttccg cgcggcgcgc
    22741 gcacgggacg cgcgccctcg ctcattcgag ccctcc
//
LOCUS       DASVXS010000314         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_919423, whole genome shotgun sequence.
ACCESSION   DASVXS010000314 DASVXS010000000
VERSION     DASVXS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919423"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VF752_17630"
     CDS             complement(<1..1012)
                     /locus_tag="VF752_17630"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8857420.1"
                     /translation="MANRLAEETSPYLLQHKDNPVDWFPWGDEAFEKARAEDKPVLVS
                     IGYSACHWCHVMERESFEDPDTATLMNELLVCIKVDREERPDVDAIYMEACQAMTGQG
                     GWPLNVFVTPEQVPFYAGTYFPPQPMRGMPSWQQVVAAIAEAWTTRQDEIRQNVENVA
                     ERLRGGALLAPSVDPLQPDSLDEAVERLREQFEPVYGGFGGAPKFPPSSAIEFLLRRG
                     DTEMSVHTLHGMANGGMYDQIGGGFARYSVDAKWLVPHFEKMLYDNALLARAYLHGWL
                     VTEDPVFRRVTTETLDWALREMRGPEGGFYSALDADSEGEEGRFYVWTPEEVREAIGS
                     DPN"
     gene            complement(1048..2466)
                     /locus_tag="VF752_17635"
     CDS             complement(1048..2466)
                     /locus_tag="VF752_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8857421.1"
                     /translation="MNFATDVVGTAPSARIALVERDREGRRREISFGEVGDRTARLAG
                     ALALRGVHRGDVVMTLIGNRPEWVYAMVACFRMGAVALPCNEQLRPKDLRERIERTSP
                     SAVVTDARNLETLAASGYDGELLAVPDEALFEPDPFPAEVMAPTDPVLITFTSGTAGE
                     PKPIRHGHRYLTGQAVQAEHWFGAREGDLCWCTAATGWSKSARNVFIAPWIRGAGALL
                     HDARFDPAERLEVAEQEGVNVLCMAPTEYRAIAKRTELRRLPPLRHAVAAGEPLNPEI
                     VRVWADATGVQIHDGYGETETGALTGMPIGPPVRPGSMGKPLPGFKLWIDDGELCADP
                     STIPTFFLDGPDDVWRTGDRVSEDEDGYLWFEGRTDDVIISAGYRIGPFEVESALVSH
                     PAVAEAAAVGAPDDERGQVVRAIVVLREGHEPGERMASELQDHVKSETAPYKYPRIVE
                     FAEALPKTASGKIKRAELRSTS"
     regulatory      2540..2674
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2715..3248
                     /locus_tag="VF752_17640"
     CDS             2715..3248
                     /locus_tag="VF752_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857422.1"
                     /translation="MAACVLALPGLAFAQTGNGSGGVSPDDPAFKPAGKAKLINGIAF
                     PPADAPPAVVAAINAANKIVTKPYRYGGGHAAGFNDSGYDCSGSVSYLLHGGGLLNSP
                     LDSSAFMKWGEAGVGKWITVYTNPGHAFAMIAGLRFDTGYRTRVARKRGAKPGSGPRW
                     DIRRSTRGFSARHPVGL"
     gene            complement(3245..>3784)
                     /locus_tag="VF752_17645"
     CDS             complement(3245..>3784)
                     /locus_tag="VF752_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857423.1"
                     /translation="HGRWLFVHNGLVNDFHAIRRDLLMALDSESVAEIQGSTDSEVVF
                     HLALSYGLEQDPIAAVESALGHVEEALRRHGIEPALQASIGVSDGERIWAVRYASADR
                     PRTLFTSADVDALRKLDPENPRLQMLREGDRVIVSEPFADLPGAWHEIPESMALIVDA
                     EGGHETRPFRPKVAIQARA"
BASE COUNT          577 a         1338 c         1262 g          607 t
ORIGIN      
        1 agttcgggtc actgccgatg gcctcccgga cctcctccgg ggtccagacg tagaagcgcc
       61 cctcctcgcc ctcggagtcg gcgtcgagcg ccgagtagaa cccgccctcg ggcccgcgca
      121 tctcgcgaag cgcccagtcc agcgtctcgg tggtgacgcg acggaagacc gggtcctcgg
      181 tcacgagcca gccgtgcagg tacgcgcgcg cgagcagcgc gttgtcgtag agcatcttct
      241 cgaagtgggg gacgagccac ttcgcgtcga ccgaatagcg cgcgaatccg ccgccgatct
      301 ggtcgtacat cccgccgttg gccattccgt ggagcgtgtg aacgctcatc tccgtatcgc
      361 cgcggcggag caggaactcg atcgccgagg agggcgggaa cttcggggca ccgccgaagc
      421 cgccgtagac cggctcgaac tgctcgcgca accgctccac ggcctcgtcg agcgagtcgg
      481 gctgtagcgg gtccaccgac ggcgccagca gcgctccccc gcgaagacgc tccgcgacgt
      541 tctcaacgtt ctggcgaatc tcgtcttggc gcgtggtcca cgcctcggcg atcgccgcca
      601 ccacctgctg ccagctcggc atgccgcgca tgggctgcgg cgggaagtac gtgcccgcgt
      661 agaacggcac ctgctccggg gtcacgaaca cgttcagcgg ccagccgccc tgcccggtca
      721 tcgcctggca ggcctccatg tagatcgcgt cgacgtccgg gcgctcctcg cggtcgacct
      781 tgatgcaaac gagcagctcg ttcatgagcg tcgccgtgtc gggatcctcg aacgactcgc
      841 gctccatcac gtggcaccag tggcaggcgg agtagccgat cgacacgagc accggcttgt
      901 cctcggcgcg cgccttctcg aacgcctcgt cgccccatgg gaaccagtcc acggggttgt
      961 ccttgtgctg gaggagatac ggggaggtct cttcggcgag gcgattcgcc atctccccga
     1021 ggctaccgat gcggcgtcac gatgacctca gctcgtggag cgcagttccg cgcgcttgat
     1081 cttgccgctc gccgtcttcg gcagcgcctc ggcgaactcg acgatgcgcg ggtacttgta
     1141 gggcgccgtc tccgacttca cgtggtcctg cagctccgag gccatgcgct cgcccggctc
     1201 gtgtccctcc ctcagcacca cgatcgcgcg caccacctgg ccgcgctcgt cgtcgggcgc
     1261 gccaacggcg gccgcctccg cgacagccgg gtgcgacacg agcgccgact ccacctcgaa
     1321 gggcccgatc cggtagcccg cagagatgat cacgtcgtcg gtgcgcccct cgaaccagag
     1381 gtagccgtcc tcgtcctcgc tgacacggtc gcccgttcgc cacacgtcgt ccggaccgtc
     1441 taggaagaag gtggggatcg tggagggatc ggcgcagagc tcgccgtcgt cgatccacag
     1501 cttgaaacca ggcagtggct tgcccatcga gccgggccgc accggcgggc cgatcggcat
     1561 gccggtgagc gccccggtct cggtctcccc gtagccgtcg tggatctgca cgccggtggc
     1621 gtccgcccat acgcgcacga tctcggggtt gagaggctcg ccggcggcga ctgcatggcg
     1681 gagcggcggc agccggcgca gctcggtgcg cttcgcgatc gcccggtact ccgtcggcgc
     1741 catgcacagc acgttcacgc cctcctgctc ggcgacctcg agccgctcgg ccgggtcgaa
     1801 gcgggcgtcg tgcagcaacg cgcccgcccc tcggatccac ggcgcgatga acacgttgcg
     1861 cgccgacttc gaccacccgg tcgcggccgt acaccagcag aggtcgccct cgcgtgcgcc
     1921 gaaccaatgc tcggcctgca ccgcctggcc ggtgaggtag cgatggccgt gccgtatcgg
     1981 cttgggctcg ccggccgtgc cggacgtgaa ggtgatcagg accgggtccg tgggtgccat
     2041 cacctcggcc gggaacgggt cgggctcgaa cagcgcctcg tcggggacgg cgagcagctc
     2101 gccgtcgtag ccgctcgcgg cgagcgtctc gaggttgcgg gcgtccgtca ctaccgcgct
     2161 tgggctagtc cgttcgatcc gctcgcgcag gtccttcgga cgcagctgct cgttgcacgg
     2221 gagcgcgacc gcgcccatac gaaagcaagc gaccatggca taaacccact cgggccggtt
     2281 gccaatcagt gtcatgacca cgtcgccgcg gtgcactccg cggagtgcca gcgcgccagc
     2341 taatcgggcc gtgcggtcac ccacttcgcc gaagctgatc tcgcggcggc gcccctcgcg
     2401 atcccgctcc acgagcgcga tccgggcgga tggagcggtg ccgacgacgt ccgtcgcgaa
     2461 gttcattcgg gcaggatcgt aaggctcttc ccgcgaatta cgggtttaag tacgacactc
     2521 cgccggacga ttgtgtatcg tgccgaccac gccgaatccg cgaaagcgga gcgggggaac
     2581 cagattcggg gcgaatcttc ccttcggggg agacacgcaa gctctttcag cgtgagcccg
     2641 tcagctaacc ccgcaggcgg acgaaagaga ccttgccttc gcgtcgcaac attacgtttg
     2701 cgctggctgc cgccatggct gcttgcgtcc ttgccctgcc cggactcgca ttcgcgcaga
     2761 ctggaaacgg atccggtggc gtttcgccgg acgatccggc gttcaagccg gcaggcaagg
     2821 cgaagctgat caacggcatc gcattcccgc cggccgacgc tccgccggca gtcgtggccg
     2881 cgatcaacgc ggcgaacaag atcgtcacca agccgtaccg ttatggcggc ggccatgcag
     2941 ccgggttcaa cgactcgggc tacgactgct ccggctcggt gagctacctg ctccacggcg
     3001 gcggcctgct caactcgccc ctcgactcga gcgcgttcat gaagtggggc gaggccggcg
     3061 taggtaagtg gatcaccgtc tacacgaacc cgggccacgc cttcgcgatg atcgccggcc
     3121 tgcgcttcga taccggctac cgcactcgcg tcgcccgcaa gaggggcgcc aagccgggca
     3181 gcgggccgcg ctgggacatc cggcgctcga cgcgcggttt ctcggcgcgc catcctgtcg
     3241 ggctttaggc cctagcctga atcgcgacct tcgggcggaa gggccgcgtc tcgtggccgc
     3301 cctcggcatc cacgatcagt gccattgact ccgggatctc gtgccaggca cccgggaggt
     3361 cggcgaaggg ctccgacacg atcacccggt cgccctcgcg cagcatctgc aggcgagggt
     3421 tctccgggtc gagcttccgg agcgcgtcca cgtcggccga cgtgaacagc gtgcgcggac
     3481 ggtccgcgct cgcatagcga acggcccaaa tgcgctcgcc gtccgaaacg ccgatgcttg
     3541 cctgcagcgc cggctcgatg ccgtggcgcc gtaatgcttc ctccacgtgt ccgagcgcgg
     3601 attcgaccgc tgcgatcgga tcttgctcga gcccgtagga gagggccagg tgaaagacca
     3661 cttccgagtc cgtggagccc tggatctcgg caacggactc ggaatcgagc gccatcagga
     3721 ggtcgcgtcg gatcgcgtgg aagtcgttga ccagtccgtt gtgcacgaag agccagcggc
     3781 cgtg
//
LOCUS       DASVXS010000315        13941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_282346, whole genome shotgun sequence.
ACCESSION   DASVXS010000315 DASVXS010000000
VERSION     DASVXS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 13941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13941
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282346"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VF752_17650"
     CDS             <1..1825
                     /locus_tag="VF752_17650"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8857424.1"
                     /translation="YAAGGDGASFDFADYGQKGNPCGDPPGPAGTNLTTPTAEGGSLR
                     SQDVVTPGDPTGLSGSVIRIEPETGQAAPGNPFSSSSDVNAQRIIAYGLRNPFRIAFR
                     PGTNDLWIGDVGNATWEELNHTNAADSTADNFGWPCWEGAAHQGSYEAAGTSLCNQIY
                     ANGGTTLPFLTYNHSNPVVPGETCRTGSGSAVTALRFSSSVAPYPSSFQGGLFFADDP
                     RQCIWYMPPGSGGIPDKNSVQVFETTAHNPVDMQIGPDNKLWYMDFDFFGGAGGSQIH
                     RIDWTPGNQAPVAKISANPTNGATPLAVSFSGTGSSDPEGSALTYRWDLNGDGTYGDS
                     TSATPSFTYATNGVYNVGLEVTDNVGQKGYDHVAVTAGNSAPTAVISTPLPSTTWKVG
                     DPINFSGSATDAQDGTEPASRLTWSVIMHHCTTPNTCHTHPIQDYAGVASGSFAAPDH
                     EYPSWIEVALTATDSGGLSNTQSVRLDPKTAVVTMQSSPASLKLAIDSSLLTAPFTKT
                     VILGSDNSVSAGSPQRLGSRTYYFRGWSDDGAQSHNLTINGNTTLTATYGRIRLGSRA
                     AAKRRAAARRRAAARRKAAKLRAERLRRARLHHRHAHHRHG"
     gene            complement(1829..3250)
                     /locus_tag="VF752_17655"
     CDS             complement(1829..3250)
                     /locus_tag="VF752_17655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8857425.1"
                     /translation="MKSRTIWTFAIVSAALFMVTLDNLVVTTALPVIRKDLGATIQQL
                     EWTVNAYTLTYAVLLLTGAALGDRFGRRRAFLGGLAIFTLGSAAAAIAPDTGALIASR
                     ALQGAGAAVVTPLTLTLLAEAVPAGRRGLALGAWSGISGLGVALGPVVGGAVVDGLSW
                     QWIFWLNVPIGLLAIPLAARHLDESFGPRERLDLRGLVLGSVGLLGVVYGVVKAQSLG
                     WSATTVLASLSAGVLLLIGFAVVERRSESPMLPLRFFQSRRFSATNGVSFAMYFGIFG
                     AIFLLAQFFQTVQGMSPLEAGLRTLPWTGMPMVIAPFAGIWSDRIGSRPLMATGLALQ
                     AIALAWIAVVTAPDTAYGLLVPAFVMAGTGMALVFAPAANSVLQAVRPDQAGKASGAT
                     NTVRELGGVFGVSVLASVFAASGGYQSAQAFVDGVVPALWVGVAVLGAGALAALAIPG
                     ISRRKPEVAALPASPRPCESQAA"
     gene            complement(3247..3816)
                     /locus_tag="VF752_17660"
     CDS             complement(3247..3816)
                     /locus_tag="VF752_17660"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8857426.1"
                     /translation="MQTLNPTARVILGMIKLGRNTGYDIKSLVDVSTRHFWAASYGQL
                     YPELKRLEQDGLITGRDEPSGGRRRRVYELTEDGERALHDWLASDEKPSYELRDEGLL
                     KIFFGDSMSADELVRAVQANRRLHEGVGAALREIEPHARDAETRFPYLTLQAGLAEHD
                     HMAAWWREVEERLARGEDPHVPADPKEQQ"
     gene            3872..4282
                     /locus_tag="VF752_17665"
     CDS             3872..4282
                     /locus_tag="VF752_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8857427.1"
                     /translation="MSSGIPVTGVSELVLYVKDLAAAERFYADALGLPVVERWGDPRA
                     AVWVMAGDRTRIGLWGHEGPGIAGGRGGAHVHFAMHIDTADLDAAIERLRSHGLEVEI
                     ADFGDSGTAAYVTDPDGNVVELWTWDVAEHLRPR"
     gene            complement(4286..4918)
                     /locus_tag="VF752_17670"
     CDS             complement(4286..4918)
                     /locus_tag="VF752_17670"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HEX8857428.1"
                     /translation="MPRSLAVALHDVEPATFARTVAIRDWLDERGVAHATLLVIPASD
                     LHLFSDRSPELLDWLRARSRAGDAIAVHGLRRGRPGRSAGEFARLPLEETRAAVQLGK
                     AIVERAGLEARGFVAPGYAYTRGLRNELGERFDWWADLLHVHSIRRRVLSPALSPVTA
                     RLLAAMPRSLLRLDIHPRDYDSPAHMHALERTLDRVERRSCVTYDEILAA"
     gene            complement(4908..7205)
                     /locus_tag="VF752_17675"
     CDS             complement(4908..7205)
                     /locus_tag="VF752_17675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8857429.1"
                     /translation="MTTRETGDAMSEGKSRADLHCHSTASQLAKLGVQRAVGLPECAT
                     RPEEVYELAKRRGMHFVTITDHDTIAGAITLADRSDFFVSEELTARFKDEPQAVHVLC
                     WGITPDDHHVLQELSGDVERCAEYLHSNEIACALAHPFYAVEAPLAPRHRRRLAELFP
                     IWETRNGSRARELNQPAAVYIETRGGTGVGGSDDHAGVDIGRTYTETPHADTVEGFLE
                     QIRAGSAKGCGEQGSAAKWAHSALAIALRVLGVGDGDGIDPRAVLMLAERVVRQGQER
                     SSDHRVDLDPDDARRLLRAWLASVELGVDAQGLLELMQSDGFSHADLYRRARRCHERK
                     LERAVNGAVRAITGGESLAPAAAALLEACTAAIPYVPPTVYLASEKSKLVERSGEPTR
                     VALFADAVGGMHGVTHTLDELRERGVPGFEVEVIGTDAHVDRRLPAAVELDVPFYPGL
                     KVGVPSLPDVAEAVAEGRYELVHLCSPGPAGVAGALVAHVMGLPVAGSYHTELAAYAR
                     VRTGDPALGEAMTATLGAFYGRCWVVMTPSESADWSVRSLGVPPERIRRWGRGVDLDR
                     FSPQRRADGLLPGAVNVLYAGRLSREKGAELLADAFLAAAARDPRLHLVLAGGGPEEE
                     RLRERLGERGTLLGWLDGDDLARVYASADVFLFASRTDTFGQVVLEAQASGLPVVAVD
                     EGGPSGLIEDRVSGRLCEPDVDVLAAAVLELAGSAALRTRFARAARDAAARHSWRHSL
                     GQLAEGWMQALGLSPAAFEETRRAA"
     gene            7443..9566
                     /locus_tag="VF752_17680"
     CDS             7443..9566
                     /locus_tag="VF752_17680"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX8857430.1"
                     /translation="MLIDRLSPWAAVGEAEDRFIHYTEGVIPHERLEPRERPERASAV
                     NGPPSGAPLHGMLHGGRTFRFAAHVADEHVTLTFDTDGDPLRIGASWALQAGEAFTGV
                     GARHGTSVDQAGRMIRLGADRRYTGPDCPPDLLEAGGIPQGDCAPVPWVISSCGWALW
                     IETSGDGVELDLGASGEVSASTRSAAGPLRLHFFTDPTPAARLRRYCSLTGYPELLPE
                     WAYGHWKSRDVYEHERDVVDDFERYREHELPLDAIVIDSPWETQYNTWEFNGRQFPDA
                     PGLIRRMRDDGVRTVVWVTPWVNLESGEGQYPPDEESERIHREPAPNYAPAAEAGHFV
                     RAADGRPYVARWWMGTGSPVDFTSKAAEAWWRGLARRVLDLGVEGIKADDGEGYYLPA
                     DTRFADGRTGAEAAWAWGGLYRESMQCALRESHGDEGVLFGRSGWAGQQRHGMLWGGD
                     QASDFWSLRALVNATLTAAASGFSNWSHDVGGYLGERLVERCPKELLIRWLQFGCFTP
                     LMHAHGRFQQEAWTYDRETLDVYRSFLLLHERLVPYVRAAAATAARTGLPIVRPLFLL
                     DRSDRHGFAISDSYGYGPALWVAPVLDDGARRRRVELPRGDWIDYWTRERLSGGREVT
                     AAAPIDRIPVFVRAGSVIVTYPEGHVARGLGDTPEAERPLEATLWGAPALGSAGARLA
                     DGTRIRWRRGSWSATPERDVTFAEL"
     gene            complement(9571..10314)
                     /locus_tag="VF752_17685"
     CDS             complement(9571..10314)
                     /locus_tag="VF752_17685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8857431.1"
                     /translation="MGRLDGKVCVITGASGGIGAASVERFREEGATVAGVDLEEGAPG
                     DLSLQADVTDEEQVRAMYQRVRDELGGIDVLFNNAGIAPPDDESVLHTPLDAWRRVQD
                     VNLTSVYLCCRHGIPHLIERGGGSVINTASFVAVMGAATSQISYTASKGGVLALSREI
                     GIEFARKGVRVNALCPGPVNTPLLQELFAKDPVQAERRRVHLPMGRFAEAHEIANGAL
                     FLASDESSYVTGATFLVDGGISGAYLTPE"
     gene            complement(10324..11625)
                     /locus_tag="VF752_17690"
     CDS             complement(10324..11625)
                     /locus_tag="VF752_17690"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX8857432.1"
                     /translation="MAGGTVDTVLLAIADMQGRLMGKRLTARHFLDEVVPHGAEGCDY
                     LLANDVEMTPVAGYEMTSWDKGYGDFEMKPDLDTLRPLPWLEGTALCMADILWADERP
                     VVASPRQILKRQLDRVAERGWTANVGTELEFLVFRNTYEDAWNKGYRDLEPANYYNVD
                     YSLLGTSRVEPLIRRIRNSMMGADMRVENSKGECNFGQHEVNFRYTQALQTADEHTIY
                     KNGAKEIASQEGCAITFMAKFNEREGNSCHIHCSLATDGGRNVFADDEQTFHRFIAGQ
                     LAAMRELTLFYAPNINSYKRFVPGSFAPTAVVWGDDNRTCSIRVVGHGQALRLENRLP
                     GADVNPYLAIAAMIAAGLHGVDNELELEPAYDGNAYESDKPRVPSTMRDARDLFAESA
                     IARAAFGDEVVDHYLNYARVELEAYDAAVTDWERYRGFERL"
     gene            complement(11649..13262)
                     /locus_tag="VF752_17695"
     CDS             complement(11649..13262)
                     /locus_tag="VF752_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8857433.1"
                     /translation="MSDATPEVLGRDERRLAELGYKQSLNRAWSGFSNFAISFTIISV
                     LAGCFTTYGQAWNNGGPIAITWGWPIISAFILIVALSMSELVSKYPTAGAIYWWSSEM
                     GGRVWGWFTGWFNMIGLIGVVASVDYACATFLNAVLGLYKVDFIFNFGDDAHILRETF
                     FLFAVILVLHALINIFQTQLLALFNNVSVWVHVIGVAVIVAILAFVPDTHQSFDFVFT
                     KRINNSGFSGGMFWFYILPLGFLLTQYTITGFDASAHISEETKDAEQAAARGVWRSVF
                     YSAVIGWIVLLAITFAATDVKAINEGAGSSLAIFDSALTPAAAKAVVLISTIGQFFCG
                     MACLTSASRMFYAFSRDRAVPGNRLWTRLTQKRVPSYAVMMVALLSLLITLPALHGDE
                     NAFPFAFFAVVSITVIGLYIAYVIPIFLRWRMGDKFQPASWTLGRKYKWMNAVAVGEV
                     AIITVVFCLPFTPAAVPWNDGFKWEAFNYAPLTVGLVLIGAWLGWILWAKKHWPGHVR
                     EVDPFGEGAEIRPDVPTTPPEPEPGSAPA"
     gene            13390..>13941
                     /locus_tag="VF752_17700"
     CDS             13390..>13941
                     /locus_tag="VF752_17700"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HEX8857434.1"
                     /translation="MSSEIAPILDRLAAVLGAPDGDPTPLDGGITNRNYAVTMDGERY
                     VVRLPGKDTELLLIDRSAELEANKAAATLGIAPPVAAMLHDPPCIVTRFLDGRVMQAE
                     ELRDPAVLAEVAGALRIFHERGPALPAVFDSFQIPIDYAATAREHGAEPPDGYLEAME
                     HATQIKAALQGPEHTRVPCHNDLL"
BASE COUNT         2213 a         4939 c         4791 g         1998 t
ORIGIN      
        1 ctatgccgcg ggcggcgacg gggcgagctt cgacttcgcc gactacggcc agaagggaaa
       61 tccctgcggg gatcccccgg gacccgccgg cacgaatctc accacgccta ccgccgaggg
      121 cggctccctg cgctcgcagg atgtggtcac gcccggcgat ccgaccgggc tcagcggctc
      181 tgtgatccgg atcgagccgg agaccggcca ggccgcgccg gggaacccgt ttagctcgag
      241 cagcgacgtg aacgcgcagc ggatcatcgc ctacggcctc cgcaacccgt tccggatcgc
      301 gttccgcccg ggcacgaacg acctatggat cggcgatgtg ggcaacgcca cctgggagga
      361 gctgaaccac acgaacgctg ccgactcgac ggccgacaac ttcggctggc cctgctggga
      421 gggagcggcg caccagggat cgtacgaggc cgcaggcaca agcctctgca accagatcta
      481 cgcgaacggc ggcacgacct tgccgttcct cacctacaac cacagcaacc cagtggtccc
      541 cggggagacc tgccggaccg gctcgggatc cgcagtcacg gctctgcgct tctcgtcgtc
      601 ggtcgcgccc tatccgtcgt cgttccaggg cggcctgttc ttcgccgacg acccgcgcca
      661 gtgcatctgg tacatgccgc cgggaagcgg gggcattccg gacaagaact ccgtccaggt
      721 gttcgagacg acggcgcaca acccggtcga catgcagatc gggccggaca acaagctctg
      781 gtacatggac ttcgacttct tcggcggcgc gggcggcagc cagatccacc gcatcgactg
      841 gacgccgggc aaccaggcgc ctgtcgcgaa aatctcagcg aaccccacga acggagcgac
      901 gccgctggcg gtgagcttca gcgggacggg ctcgtccgac cccgagggca gcgcgctcac
      961 ctaccgctgg gatctgaacg gggacgggac atacggtgac tcgacttcgg ccacgccgtc
     1021 gttcacctac gcgaccaacg gcgtctacaa cgtcgggctc gaagtcaccg acaacgtggg
     1081 ccagaagggc tacgaccacg tcgcggtcac ggcaggcaac agcgccccta ccgcggtgat
     1141 cagcaccccg cttccctcca ccacctggaa ggtcggcgac ccgatcaact tctccgggag
     1201 cgccaccgat gcgcaggacg gcaccgagcc ggcctcacgc ctcacctggt cggtgatcat
     1261 gcaccactgc acgacgccca acacctgcca tacgcatccg atccaggact atgcgggcgt
     1321 ggcctcgggc tcgttcgccg cgccggacca cgagtacccg tcttggatcg aggtggccct
     1381 cacagccacc gactccggcg ggctgagcaa cacgcagagc gtgcgcctcg acccgaagac
     1441 ggcggtggtg acgatgcagt cgagccctgc cagcctgaag ctcgcgatcg attcgagcct
     1501 gctgaccgcg ccgttcacga agaccgtgat ccttggctcg gacaactcgg tcagcgcggg
     1561 cagcccgcag cggctcggca gccgcacgta ctacttccgc ggctggtccg acgacggcgc
     1621 gcagagccac aacctcacga tcaacggcaa cacgacgctc accgccacct acggccgtat
     1681 ccggctcggc tcgcgagcgg cggcgaagcg tcgcgcagcc gccaggcggc gggcggccgc
     1741 ccgccgaaag gcagcgaagc tgcgcgccga acggctccgg cgcgcgcgcc tccatcacag
     1801 gcacgcgcac cacaggcacg gctgagcctc aggcggcctg agactcgcag ggccgcggcg
     1861 acgcgggcag cgccgcgacc tcgggcttcc ggcggctgat cccggggatc gcaagcgccg
     1921 cgagtgcgcc cgcgccgagc acggccacgc ccacccagag tgcggggacg accccgtcga
     1981 cgaaggcctg cgcggactgg tagccgcccg aggccgcgaa caccgacgcg agcacggaca
     2041 cgccgaacac gccgccgagc tcgcgcacgg tgttcgtggc gccgctcgcc ttcccggctt
     2101 ggtccggccg aacggcctgg agcaccgagt tggcggccgg cgcgaacacg agcgccatcc
     2161 ccgtgcccgc catcacgaac gccggaacga gcagcccgta ggcggtgtcc ggcgccgtca
     2221 cgacggcgat ccacgcgagt gcgatcgcct gcagcgcgag tcccgtggcc atcagcgggc
     2281 ggctgccaat ccggtccgac cagatgccgg cgaacggcgc aatcaccatc ggcataccag
     2341 tccacggcag tgtgcgcagc ccggcctcga gcggcgacat gccctgcacg gtctggaaga
     2401 actgggcgag caggaagatc gccccgaaga tgccgaagta catggcgaac gagacgccat
     2461 tcgtggcgct gaacctgcgg ctttggaaga agcgcagcgg cagcatcggg ctctcggacc
     2521 gccgctcgac gacggcgaaa ccgatgagga gcaagacgcc ggcggacaac gaggccagca
     2581 cggtcgttgc gctccagcca agtgactgcg ccttcacgac tccgtacacc acgccgagga
     2641 ggccgacgct gccgagcacg aggcctcgca ggtcgaggcg ctcgcgcggc ccgaagctct
     2701 cgtccaggtg tcgtgcggcg agcgggatgg cgagcagccc gatcggcacg ttcagccaga
     2761 agatccactg ccacgagagg ccgtccacga cggcgccgcc gacaaccggg ccgagcgcga
     2821 cgccgaggcc gctgatgccc gaccaggcgc cgagggccag gccccgccgg cccgcgggca
     2881 ccgcttcggc tagcagcgtg agggtgagcg gggtcaccac tgccgcgccc gcgccctgca
     2941 gcgcgcgcga ggcgatcagc gccccggtgt ccggggcaat tgccgccgcg gcggagccga
     3001 gcgtgaagat cgccaggccg ccgaggaacg cgcgccggcg gccgaagcgg tcgccgagcg
     3061 cggcgccggt gagcagcagg acggcgtagg tgagcgtgta cgcgttcacc gtccactcga
     3121 gctgctggat cgtggcgccg aggtctttgc ggatcaccgg cagcgccgtg gtcactacga
     3181 ggttgtcgag cgtgaccatg aagagggccg ccgagacgat cgcgaatgtc cagatggtgc
     3241 gcgacttcat tgctgctcct tggggtcggc cggcacgtgc gggtcctccc cgcgcgcgag
     3301 tcgctcctcg acttctcgcc accacgcggc catgtggtcg tgctcggcga gtccggcttg
     3361 gagggtgagg tacggaaagc gcgtctccgc gtcgcgtgcg tgcggctcga tctctcgcag
     3421 ggccgcgccg actccctcgt ggagccggcg gttcgcctgc acggcacgca ccagctcgtc
     3481 cgcgctcatc gagtccccga agaagatctt caggaggcct tcgtcgcgga gctcgtagga
     3541 cggcttctca tccgacgcga gccagtcgtg cagcgcacgc tcgccgtcct cggtgagctc
     3601 gtacaccctg cggcgcctgc cccccgaggg ctcgtcgcgc ccggtgatga ggccgtcctg
     3661 ctcgagccgc ttgagctcgg gatacagctg tccgtagctc gccgcccaga aatggcgggt
     3721 tgagacgtcc accagcgact tgatgtcgta gccggtgttc cggccgagct tgatcatccc
     3781 gaggatcacg cgtgccgtgg gattgagcgt ttgcatgtat cggtatgata tatcaagccg
     3841 atacattgtc aagcggatac cgtcccgccc tgtgagcagc ggcatcccgg tcaccggcgt
     3901 cagcgagctc gtcctgtacg taaaggacct ggcagctgcc gagcgcttct acgccgacgc
     3961 gctcggccta ccggtggtgg agcgctgggg cgatccccgc gccgcggtct gggtcatggc
     4021 cggcgaccgc acgcgaatcg ggctctgggg ccacgaaggg ccgggcatcg cgggcggccg
     4081 cggcggcgcg cacgtgcact tcgcgatgca catcgacacc gccgacttgg acgccgcgat
     4141 cgagcgcctg aggtcgcacg gactcgaggt cgaaatcgcg gacttcggcg acagcggcac
     4201 cgccgcctac gtcactgacc cagacggcaa cgtcgtcgag ctatggacct gggacgtcgc
     4261 cgagcacctg cgcccgcgtt gaggattacg cggcgaggat ctcgtcgtag gtaacgcacg
     4321 aacggcgctc tacgcgatcg agagtccgct ccagcgcgtg catgtgggcc ggcgagtcgt
     4381 agtcgcgggg atggatatcg agccgaagta gcgaccgcgg catcgcggcg agcagccgtg
     4441 cggtgaccgg agacagcgcc ggcgacagca cgcggcgccg gatgctgtgc acgtgcagca
     4501 ggtccgccca ccagtcgaag cgctcgccca gctcgttgcg gaggccacgc gtgtacgcgt
     4561 acccgggtgc aacgaagccg cgcgcttcga ggcccgcgcg ctcgacgatc gccttgccga
     4621 gttggaccgc cgcccgcgtc tcctcgagcg gcaggcgcgc gaactccccc gcggaccggc
     4681 ctggccggcc acgcctcagc ccatgcaccg cgatcgcgtc gcccgcccgc gagcgggcgc
     4741 gcagccagtc gagcagctcg gggctgcggt cgctgaagag gtgcaggtcc gaggccggga
     4801 tcaccagcag cgtggcgtgc gccacgccgc gctcgtcgag ccagtcgcgg atagccacgg
     4861 tgcgcgcgaa cgtggccggc tccacgtcgt gaagagcgac ggccaggcta cgcggcacgg
     4921 cgagtctcct cgaaggccgc cggcgagagc ccgagcgcct gcatccagcc ctcggccagc
     4981 tgcccaagcg agtgccgcca gctgtggcgg gcagccgcgt cgcgggcggc tcgcgcgaaa
     5041 cgtgtgcgga gtgcggcgga tcccgcgagc tcgagcaccg cagcggcgag cacatccacg
     5101 tcgggctcgc acagccggcc gctcacgcga tcctcgatca ggccgctcgg tccgccctcg
     5161 tcgaccgcca ccaccggcag gcccgaggcc tgagcctcga ggacaacctg gccaaacgtg
     5221 tccgtgcggc tcgcgaacag gaacacgtcg gcactggcgt acacgcgcgc gagatcgtcg
     5281 ccgtccagcc agccgagcag cgtgccccgc tcgccgagcc gctcgcgcag gcgctcctcc
     5341 tcgggcccgc cgccggcgag cacgaggtgc agtctcggat cacgtgccgc cgcggcgagg
     5401 aacgcgtccg ccagcagctc cgctcccttc tcgcggctca ggcggccggc gtacaggacg
     5461 ttcaccgcgc ccgggagcaa tccgtcggcg cgccgctgcg gcgagaagcg gtcgagatct
     5521 acgccgcgac cccagcgccg gatgcgctcg ggcggcacgc ccagcgagcg cacggaccag
     5581 tccgcggact cgctcggcgt catgaccacc cagcacctgc cgtagaacgc cccgagcgtg
     5641 gcggtcatcg cctcgccgag cgccgggtcg ccggtccgca cgcgcgcgta ggcggcgagc
     5701 tcggtgtggt agctgcccgc taccggcagc cccatcacgt gcgccacgag cgcacccgcg
     5761 actcccgcgg gtccgggcga acagaggtgc acgagttcat accgcccctc ggcgacggcc
     5821 tccgccacgt cggggaggct cggcacgccc accttcaggc ccgggtagaa gggcacgtcc
     5881 agctccaccg ctgccgggag gcggcggtcg acgtgcgcgt cggtgccgat cacctccacc
     5941 tcgaagccgg gcacgccgcg ctcgcgcagt tcgtccaggg tgtgagtgac gccgtgcatg
     6001 ccgcccaccg cgtccgcgaa cagcgcgacc cgggtgggct cgcccgacct ctccaccagc
     6061 ttcgacttct cgctcgcgag ataaacggtc ggcggcacgt agggaatcgc cgccgtgcag
     6121 gcctcgagca gtgcggccgc agcgggcgcg aggctctcgc cgcccgtgat cgcccgcacc
     6181 gcgccgttca cggcgcgttc cagtttgcgc tcgtggcagc ggcgagcccg ccggtacagg
     6241 tcagcgtggg agaagccgtc cgactgcatc agctcgagga ggccctgggc gtctacgccg
     6301 agctcgaccg acgcaagcca cgcgcgcaag agccgccgcg cgtcgtcggg gtcgagatcc
     6361 acgcggtggt cggagctccg ctcctggcct tggcgtacga ctcgctcggc gagcatcagc
     6421 acggcgcgcg ggtcgatccc gtccccgtcc cccacaccga gaacccgcag cgcgatcgcg
     6481 agcgcagagt gggcccactt cgcggcgctg ccctgctcgc cgcagccctt ggcgctgcct
     6541 gcacggatct gctcgaggaa accctccacc gtgtcggcgt gcggggtctc ggtgtaggtc
     6601 cggccgatgt cgacgccggc gtggtcgtcg gagccgccga cgcccgttcc gccgcgtgtc
     6661 tcgatgtaga cggcggcggg ctggttcagc tctcgcgcgc gggagccatt gcgtgtctcc
     6721 cagatcggga agagctcggc aaggcgccgg cggtggcgcg gcgccagcgg cgcctccacc
     6781 gcgtagaacg gatgcgcgag cgcgcacgcg atctcgttcg agtgcaggta ctccgcacag
     6841 cgctcgacgt cgccgctgag ctcctgaagc acgtggtggt cgtccggcgt gatgccccag
     6901 cagagcacgt gcacggcctg cggctcgtcc ttgaaccgcg cggtgagctc ctccgagacg
     6961 aagaagtcgc tgcgatcggc gagcgtgatg gcgcccgcga tcgtgtcgtg gtcggtgatg
     7021 gtcacgaagt gcatcccccg ccgcttggcg agctcgtaca cctcttccgg ccgtgtcgcg
     7081 cattccggca gacccaccgc ccgctgcacc ccgagcttcg ccagctgcga ggccgtcgaa
     7141 tggcagtgca gatcggctcg gctcttcccc tcgctcatcg cgtccccagt ctcccgtgtc
     7201 gtcatgggtg cagatttgcc tgcaaacacc gccttttcgt tacgggacgg aaaacgagac
     7261 gtgaccagat ggtgaacgcg gcgcgttaac aaaacggaca ccggatccgc gccggtggcg
     7321 agaaaagctc tggcatcgca cttcagagga ccgatccgca acctcgaggc gtcgcgctga
     7381 gcaccggccc gctcgcgatc tcgctcgagc cgctgtcgct ctcgatccgg cgagcgggcc
     7441 gcgtgctcat cgaccggctc tcgccgtggg ccgcggtggg cgaggccgag gatcgcttca
     7501 tccactacac cgagggcgtg atcccgcacg agcggctcga gcctcgcgag cgtccagagc
     7561 gcgcttccgc cgtcaacggg ccgccgagcg gcgcgccgtt gcacggaatg cttcacggtg
     7621 ggcggacgtt tcggttcgcg gcccatgtcg cggacgagca cgtcaccctc acgttcgaca
     7681 ccgacggcga cccgcttcga atcggggcca gctgggcgct tcaggcgggc gaggcgttca
     7741 ccggcgtcgg cgcccgccac ggcacgagcg tcgatcaggc gggacgcatg atccggctcg
     7801 gcgccgatcg ccgctacacc gggccggact gcccgcccga cctgctcgag gcgggcggaa
     7861 tcccgcaggg cgactgcgcc ccggtgccat gggtgatctc gagctgtggc tgggcgctct
     7921 ggatcgagac gagcggagat ggcgtggagc tcgacctcgg tgcgtccggc gaggtctcag
     7981 catccacccg ctcggcggcc ggcccgcttc ggctgcactt cttcaccgat ccgacccccg
     8041 ccgcgcggct ccgccgctac tgctcgctca ccggctaccc ggagctgctc cccgagtggg
     8101 cctacggcca ctggaagagc cgcgatgtgt acgagcacga gcgcgacgtg gtcgacgact
     8161 tcgagaggta tcgcgagcat gagctgccgc tcgacgcgat cgtgatcgac tcgccgtggg
     8221 agacgcagta caacacctgg gagttcaacg gcagacagtt ccccgacgcg cccggactca
     8281 tccggcgcat gcgcgacgat ggcgtgcgca ccgtcgtgtg ggtcactccg tgggtgaacc
     8341 tcgagtcggg cgagggccag tacccgccgg acgaggagtc cgagcggata caccgcgagc
     8401 cggctccgaa ctacgcgccc gcggcggagg ccgggcactt cgtgcgcgcg gcagacggcc
     8461 gcccgtatgt tgcgcgctgg tggatgggca ccggctcgcc ggtggacttc acgtcgaagg
     8521 ccgccgaggc gtggtggcgc gggctcgcgc ggcgggtgct cgatcttggc gtcgagggca
     8581 tcaaggccga cgatggcgag gggtactacc tgccggccga cacccgcttc gccgacgggc
     8641 gcaccggtgc cgaggcggcg tgggcatggg gcgggctcta ccgcgagtcg atgcagtgcg
     8701 cgcttcgcga gtcacacggc gacgagggcg ttctcttcgg ccggagcggc tgggcggggc
     8761 agcagcgaca cggaatgctc tggggcggcg accaggcgtc cgacttctgg tcgctacggg
     8821 cgctcgtgaa cgcgacgctc accgccgccg cgagcggctt ctcgaactgg tcacacgacg
     8881 tgggcggcta cctcggggag cggctcgtgg agcgctgccc caaggagctc ctgatccgat
     8941 ggctgcaatt cggctgcttc acgccgttga tgcacgcaca cggccgcttc cagcaggagg
     9001 cgtggaccta cgaccgcgag acgctcgacg tctaccggtc attcctgctg ctccacgaac
     9061 gcctcgtgcc ttatgtgcgg gccgcggctg ccacggccgc gcgcacgggg ctgcccatcg
     9121 tccggccgct gttcctgctc gatcgcagcg accgccacgg gttcgccatt tccgactcct
     9181 acggctacgg gccggcactt tgggtcgcgc cggtactcga cgacggcgcc cggcgccggc
     9241 gtgtcgagct gccgcggggt gactggatcg actactggac gcgcgagcgg ctgagtggcg
     9301 ggcgcgaggt gacggccgcc gcgccgattg accggatccc ggtcttcgtc agagcgggca
     9361 gcgtcatcgt gacctacccc gagggccacg tggcgcgagg tctcggcgat acgccggagg
     9421 ccgagcgtcc gctcgaggcg acgctgtggg gcgcgcccgc gctgggatcc gcgggtgcgc
     9481 ggctcgccga cggcacgcgc atacgctggc gccgcggcag ctggagcgcg acgcccgagc
     9541 gggacgtgac gttcgccgag ctgtagagcg ctattccggc gtgagatatg cgcccgagat
     9601 gccgccgtcc acgaggaacg tcgcgcccgt gacgtacgac gattcgtcgc tcgcgagaaa
     9661 cagcgcgccg ttcgcgatct cgtgagcctc ggcgaagcgg cccatcggca ggtgcacgcg
     9721 ccggcgctcc gcctgcacgg ggtccttcgc gaacagctcc tgcagcagcg gcgtgttgac
     9781 tggccccggg cacagcgcgt tcacgcgcac acccttgcgc gcgaactcga tgccgatctc
     9841 gcgcgagagc gcgagcacgc cgcccttcga tgcggtgtag gagatctgcg acgtggccgc
     9901 gcccatcaca gcaacgaacg acgcggtgtt gatcacggag ccgccgccgc gctcgatcag
     9961 gtgcgggatg ccgtgccggc agcagaggta gacgctcgtg aggttcacgt cctgcacgcg
    10021 tcgccaggcg tcgagcgggg tgtgaagcac ggactcgtcg tcgggcggcg cgatgccggc
    10081 gttgttgaag agcacgtcga ttccgccgag ctcgtcgcgc acacgctggt acatcgcgcg
    10141 cacctgctcc tcgtccgtca cgtcggcctg cagcgatagg tcgccggggg cgccctcctc
    10201 gaggtccacg cctgccacgg tcgcgccttc ttccctgaag cgctcgaccg atgcggctcc
    10261 gatgccgccg gacgcgcccg tgatcacgca gaccttgccg tcaagccgcc ccatccaacc
    10321 tcctcacagt cgttcgaatc ctcggtaccg ctcccagtcc gtgaccgccg cgtcgtacgc
    10381 ctcaagctcc acgcgcgcgt agttgaggta gtggtcgacc acctcgtcgc cgaacgcggc
    10441 gcgggcgatc gcgctctcgg cgaacaggtc gcgcgcatcg cgcatcgtgc tcggcacgcg
    10501 cggcttgtcg gactcgtagg cgttcccgtc gtaggccggc tcgagctcga gctcgttgtc
    10561 cacgccgtgc aggcccgcgg cgatcatcgc ggcgatcgcc agatacgggt tcacgtccgc
    10621 gcccggcaac cggttctcca ggcgaagcgc ctggccgtgg cccactacgc gtatcgagca
    10681 cgtccggttg tcgtcgcccc agacgacggc ggtcggcgcg aacgatcccg gcacgaagcg
    10741 cttgtacgag ttgatgttgg gcgcgtagaa gagcgtgagc tcgcgcatcg cggcgagctg
    10801 gcccgcgatg aagcgatgga acgtctgctc gtcatccgcg aacacgttgc ggccgccgtc
    10861 ggtggccagc gaacagtgga tgtggcacga gttgccctcg cgctcgttga acttcgccat
    10921 gaacgtgatc gcgcagccct cctgcgacgc gatctccttg gcgccgttct tgtagatcgt
    10981 gtgctcgtcc gcggtctgga gcgcctgggt gtagcggaag ttcacctcgt gctggccgaa
    11041 gttgcactcc cccttcgagt tctccacgcg catgtcggcg cccatcatcg agttgcgtat
    11101 gcgccggatg aggggctcga cgcgtgaggt gccgagcagc gagtagtcga cgttgtagta
    11161 gttggccggc tcgagatcgc ggtagccctt gttccaggcg tcctcgtacg tgttgcggaa
    11221 cacgaggaac tcgagctccg tgcccacgtt cgccgtccag ccgcgctcgg cgacgcggtc
    11281 gagctggcgc ttcaggatct ggcgcggcga agcgaccacc ggccgctcgt cggcccagag
    11341 aatgtcggcc atgcagagcg cggttccctc gagccacggc agcggccgca gcgtgtcgag
    11401 gtccggcttc atctcgaagt ccccgtagcc cttgtcccac gaggtcatct cgtagccggc
    11461 gaccggcgtc atttcgacgt cgttcgcgag caggtagtcg cagccctcgg cgccatgcgg
    11521 cacgacctcg tccaggaagt gccgcgcggt gaggcgcttg cccatgagcc gcccctgcat
    11581 gtcggctatc gcgaggagca cggtgtcgac cgtgcccccc gccactgcct gtctcagctc
    11641 atcgagcgtc acgcaggcgc tgaacccggc tccggctcgg gcggcgtggt gggcacgtcc
    11701 ggccggatct cagcgccctc cccgaagggg tccacctcgc ggacgtggcc gggccagtgc
    11761 ttcttggccc acaagatcca gccgagccag gccccgatca gcacgaggcc cacggtgagt
    11821 ggggcgtagt tgaacgcctc ccatttgaag ccgtcgttcc acgggaccgc ggcgggagtg
    11881 aacggcaggc agaagaccac ggtgatgatc gccacctcgc cgaccgcgac cgcgttcatc
    11941 cacttgtact tcctgccaag tgtccacgag gccggctgga acttgtcccc catcctccag
    12001 cgcaggaaga tcgggatcac gtacgcgatg tagagcccga tgaccgtgat cgacacgacc
    12061 gcgaagaacg cgaacgggaa cgcgttctcg tcgccgtgca gggcgggcag cgtgatgagc
    12121 agcgacagca gcgcgaccat catcaccgcg taggacggca cgcgcttctg cgtgagccgc
    12181 gtccacaggc ggttgcccgg gacggcccgg tcacgcgaga acgcgtagaa catccgcgag
    12241 gcgctcgtga ggcacgccat gccgcagaag aactgaccga tcgtggagat caggacgacc
    12301 gccttcgccg cggcgggcgt cagcgcactg tcgaagatcg cgagcgacga gcccgcgccc
    12361 tcgttgatcg ccttcacgtc ggtggcggcg aacgtgatcg cgagcaacac gatccatccg
    12421 atgaccgccg agtagaacac cgaccgccac acgcccctgg cggccgcctg ctcggcgtcc
    12481 tttgtctcct ctgagatgtg cgcggaggcg tcgaagccgg tgatcgtgta ctgcgtgagc
    12541 aggaagccga gcggcaggat gtagaaccag aacatccctc cgctgaagcc ggagttgttg
    12601 atgcgcttcg tgaacacgaa gtcgaagctc tggtgcgtgt cgggcacgaa ggcgaggatc
    12661 gcgacgatca ccgccacgcc gatgacgtgc acccagaccg agacgttgtt gaaaagggcc
    12721 aggagctgcg tctggaagat gttgatcagc gcgtggagca cgaggatgac cgcgaacagg
    12781 aagaacgtct cgcgcaggat gtgcgcgtca tccccgaagt tgaagatgaa gtcgaccttg
    12841 tacaggccga gcaccgcgtt gaggaacgtc gcgcaggcgt agtccaccga ggcgaccacg
    12901 ccgatcaggc caatcatgtt gaaccagccc gtgaaccagc cccagacgcg tccgcccatc
    12961 tccgacgacc accagtaaat cgcccccgcg gtcgggtact tcgagacgag ctcggacatc
    13021 gagagcgcca cgatgaggat gaaggcggag atgatgggcc agccccacgt gatggcgatt
    13081 ggcccgccgt tgttccaggc ctggccgtag gtggtgaagc acccggccag cacggagatg
    13141 atcgtgaatg agatcgcgaa gttggagaac ccgctccaag cgcgattgag gctctgcttg
    13201 tacccgagtt cggcaagccg tcgctcgtcg cgacccagga cttccggcgt agcgtcagac
    13261 acgcgagtgc ccctcctcgt gatcggaaag ccggactcga cctcgcaccg taaaggtgtg
    13321 atgatccgac ctctgcgggt gcgaccttaa gcgacgttgc ggacataagt caacggtttc
    13381 tcgcctccct tgagctccga aatagcgccg attctcgacc gcctggccgc cgtcctcggc
    13441 gcgcccgacg gcgatccgac gccgctcgac ggtgggatca caaaccgcaa ttacgcggtg
    13501 acgatggacg gcgagcgcta cgtcgtgcgg cttccgggga aggacacgga gctcctcctg
    13561 atcgaccgct cggccgagct cgaggcgaac aaggcggccg ccacgctcgg catcgctccg
    13621 ccggtagccg ccatgttgca cgacccgccg tgcatcgtca cgcgcttcct cgacggccgc
    13681 gtgatgcagg ccgaggagct gcgcgacccg gccgtgctag ccgaagtggc cggcgcattg
    13741 cgcatcttcc acgagcgcgg cccggcgctc ccggcggtat tcgactcctt ccagataccg
    13801 atcgactacg cggcgaccgc gcgcgagcac ggcgcggagc cgccggacgg ctatctcgag
    13861 gcgatggagc acgcgacgca gatcaaggcc gcgctgcagg gacccgagca cacgcgcgtg
    13921 ccctgccaca acgacctcct c
//
LOCUS       DASVXS010000316         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_131636, whole genome shotgun sequence.
ACCESSION   DASVXS010000316 DASVXS010000000
VERSION     DASVXS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131636"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..507)
                     /gene="msrB"
                     /locus_tag="VF752_17705"
     CDS             complement(112..507)
                     /gene="msrB"
                     /locus_tag="VF752_17705"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8857435.1"
                     /translation="MAEKVTKNEQQWREELTPEQYEVLRLKGTERPFTGIYNDEKSPG
                     TYRCAGCGAELFSSDAKFESGSGWPSFYEPSADDAVEVHEDNSHGMRRIEVTCASCGG
                     HLGHVFPDGPAPTGLRYCINSASLDLDRD"
     gene            complement(467..1432)
                     /locus_tag="VF752_17710"
     CDS             complement(467..1432)
                     /locus_tag="VF752_17710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8857436.1"
                     /translation="MSATARTWIALATVYVVWGSTYLGIRVVVKTMPPLLAGGVRFML
                     AGSIMCAALAVKRGRGYLRVSRDELIGAALVGSLLVTGGNGVVMVAEQHVASGLAALI
                     MASIPLWVVLFRRVWGESIQPAVLAGVAIGFAGVALLVGPGANGGSSAGWLVVLVGAA
                     FCWALGTFGAGRVEIPRDPLLATGWQMLLGGLLSLAIGLAVGEAADVRFSEFSSDSLW
                     AFAYLVVFGSLVAFTAYTYLLQNVPASKAATYAYVNPVIAVFLGWLILDESITWLVVV
                     AAAVIVSSVAVIVRHETTQEPEAALVPAEGRSDGREGDEERAAVA"
BASE COUNT          268 a          575 c          531 g          220 t
ORIGIN      
        1 ccccgcgctg gcggagtacg tgctcgagct gatcgagggc cgggccgcgc cagacccacg
       61 cttcggcctc ggcgcgcgaa acgccgaccg cagcctacgg acggccggct ctcagtcgcg
      121 atcgaggtcg agcgacgccg agttgatgca gtagcgcagt ccggtcggcg ccgggccgtc
      181 ggggaagacg tgcccgagat gtccgccgca gctcgcgcac gtgacctcga tgcggcgcat
      241 gccgtgcgag ttgtcctcgt gcacctcgac cgcgtcgtcc gccgacggct cgtagaagct
      301 cggccagcct gagccggact cgaacttcgc gtcggaactg aacagctccg cgccgcagcc
      361 cgcgcagcgg tatgtgccgg gcgacttctc gtcgttgtag atgccggtga acggccgctc
      421 cgtgcccttc agccgcagca cctcgtattg ctcgggcgtg agctcctcac gccactgctg
      481 ctcgttcttc gtcaccttct ctgccatctg atcttccttc cgccgggacg agtgccgcct
      541 ccggctcttg cgttgtctca tgtctcacga tcacggccac cgacgagacg atcaccgcgg
      601 cggccacgac cactagccag gtgatgctct cgtcgagtat gagccagcct agaaacacgg
      661 cgatcacggg gttcacgtac gcgtaggtgg cggccttcga cgcgggcacg ttttgaagca
      721 ggtacgtgta ggcggtgaac gccacgagcg agccgaagac cacgaggtag gcgaacgccc
      781 acagcgagtc ggacgagaac tcgctgaagc gcacgtctgc cgcctcgccg acggccaggc
      841 cgatcgcgag tgacagcagg ccgccgagga gcatctgcca gcccgtggcg agcagcgggt
      901 cgcgcgggat ctccacgcgc ccggccccga acgtgccgag cgcccagcag aacgcggcgc
      961 cgaccagaac cacgagccag ccggcgctcg agccgccgtt tgcgcccggg cccacgagca
     1021 gcgcgacgcc cgcgaagccg atcgccacgc cggcgagcac cgcgggctgg atgctctcgc
     1081 cccagacgcg cctgaacagc acgacccaaa gcgggatcga cgccatgatc agcgccgcga
     1141 ggccggaggc cacatgctgc tccgcgacca tcaccacgcc gttgccgccg gtcacgagca
     1201 gcgagcccac gagcgcggct ccaatcagct cgtcgcgcga cacgcgcagg tagccgcggc
     1261 cgcgcttcac ggcgagcgct gcgcacatga tcgacccggc cagcatgaag cgcacgccgc
     1321 ccgcgagcag cggcggcatc gtcttcacga ccacccggat tccgaggtag gtggatcccc
     1381 agacgacgta gacggtggcg agcgcaatcc aggtgcgcgc ggtggcggac acacactgag
     1441 gttggcacag cgtccgcgcc gcccttactt tgagcacggt gaagctgcac gtgctctaca
     1501 ggtcggtgga cgccgagaac cagaaaggcc gcccgccctg gtactcgaag gacgtgtcgc
     1561 tcgtgtcgct gctgcgcgcg ctcgagggct gcga
//
LOCUS       DASVXS010000317        12813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_629201, whole genome shotgun sequence.
ACCESSION   DASVXS010000317 DASVXS010000000
VERSION     DASVXS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629201"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VF752_17715"
     CDS             complement(<1..1025)
                     /locus_tag="VF752_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8857437.1"
                     /translation="MSRRSSAALVLVVGAITLGLGIAVVITAFPRGLAVLACVALAAA
                     AAWYGVLRRGIARVGGLVVAGLALAGVIALLLVGGSLLVWLLVASGVLVSVAGSERSI
                     AVHAALPAAAAPKRPVLFFNPRSGGGKAERFSLAEQARARGIEPIELTPGENLETLVR
                     RAVDGGADALAMAGGDGSQAIVAALAADHGLPYACVPAGTRNHFALDLGVDRDDVVGA
                     LDAFVDGGERRVDLAEVNGRVFVNNVSLGVYAEAVQRGGYRDAKLRTLLDTVPDVLGP
                     DGSELDLRWTGPGGHAHSGGAAVLVSNNRYRLGRVVASGTRPRLDDGLLGITVVGSPS
                     ARDAGRLL"
     gene            1040..1594
                     /locus_tag="VF752_17720"
     CDS             1040..1594
                     /locus_tag="VF752_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8857438.1"
                     /translation="MSDRREPSWIEDAKRLDLAVYSAIASTPTPELDAAMSRLSRAAD
                     YSRLSLASAALLALAGGRRGRRAAAKGLASLGVTATVVNVVVKPLGRRHRPDRVGSDV
                     PLARHVPMPTTRSFPSGHSASAFAFATGVGHVMPAAGVPLRALAALVAYSRIHTGVHY
                     PGDVLAGALMGTALAQVTTKLLDR"
     gene            1994..3183
                     /locus_tag="VF752_17725"
                     /pseudo
     CDS             1994..3183
                     /locus_tag="VF752_17725"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
     gene            3196..4086
                     /locus_tag="VF752_17730"
     CDS             3196..4086
                     /locus_tag="VF752_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX8857439.1"
                     /translation="MCRWLAYSGSPVLMEELLYKPQHSLIVQSLHSQLGAEETNGDGF
                     GVGWYGELSTPAVFRSIEPAWNDRNLRELSGHIRSGVVFAHIRASTGSPVQQTNCHPF
                     RHGRWLWMHNGLIHDFLKVKRDLVLEIDPSVYPDMEGTTDSEVIFHLALTFGLEDDPP
                     TAVERAVGLVEAVGARHGVEHPMQGTMATTNGETLWAFRYSSEGKSRSLFFSTDVRTL
                     KAQHPEIPALHELSDESRLVVSELLGDLAGAWHEVPESSYGVVGPGQAELRPFTPRPP
                     EHASNPKVGGARLERATSCL"
     gene            4209..4502
                     /locus_tag="VF752_17735"
     CDS             4209..4502
                     /locus_tag="VF752_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857440.1"
                     /translation="MIGVLVTFTQTDKFDPSTVAKIADEERGTFEGMPGLRLKSFTLD
                     DDGRARNFYLWDDEEKARAFFTEEVVDMVTGIYGVAPKIEYLDVLEFVDNSGT"
     gene            complement(4640..4712)
                     /locus_tag="VF752_17740"
     tRNA            complement(4640..4712)
                     /locus_tag="VF752_17740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4677..4679),aa:Val,seq:tac)
     gene            4770..5519
                     /locus_tag="VF752_17745"
     CDS             4770..5519
                     /locus_tag="VF752_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857441.1"
                     /translation="MAFVLTLIGAALAVGLEMLEALAIVLAVGLERRFRDSLLGTAGA
                     AAALAALAVVLGPVLLSRVPLEPLRVVIGAFLLLFGLEWLRKGVLRLAGRRSRSDSFK
                     EFVEEREALHELPPPPPGEADWAARAIAFKGVLLEGVEVILIVTALGARPGGLAPALI
                     GGVVAIAAVIGIGLVAHRPLSRLPETHLKYTVGVVLSTFGVFFAAEGLGVEWPGSDAA
                     LLYIALAFVLLSQLQIAVISQRGRTAEAVAS"
     gene            5516..5749
                     /locus_tag="VF752_17750"
     CDS             5516..5749
                     /locus_tag="VF752_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857442.1"
                     /translation="MKTLRQLRKLVLGETWSLPLGIGIALGIAALIHEVAGEWWEQAG
                     GFILLGLAALALSISLGGVLIAHASQGRSARRR"
     gene            complement(5751..5823)
                     /locus_tag="VF752_17755"
     tRNA            complement(5751..5823)
                     /locus_tag="VF752_17755"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5788..5790),aa:Glu,seq:ttc)
     gene            5936..6328
                     /locus_tag="VF752_17760"
     CDS             5936..6328
                     /locus_tag="VF752_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857443.1"
                     /translation="MQALPILWLATPFITGVCIASSHAARRFTGRHYVGCLLAGWAGV
                     TLLLAAAFLLDGPIGTAALIAGGPLAGLSFWLPRRGNDEDGGDEPADEPSPTPPDGDW
                     DRFLSDFWTYVDHRRRPSSPAPRERVSA"
     gene            complement(6339..7268)
                     /locus_tag="VF752_17765"
     CDS             complement(6339..7268)
                     /locus_tag="VF752_17765"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8857444.1"
                     /translation="MAARIRLRAAVTVTAAIALLAVAGCGGTSSGGESANKKPKPVVT
                     LGTKNFAEQYLLGQLYAQALRARGFRVVVKEDIGASEIIHRTLQAGAIDMYPEYIGVL
                     VTEVAHRSRRPSSAAQAYRLADGFEQGRGFTLLAPTPYSNTDALAVKPSYAKRWKLRT
                     VADLAKVSGSVRLAAPPEFRTRFEGLVGLEQVYGLTNIRFVPSKIGEQYGELDKGKVD
                     VADVFTTDGQLNGSRYTVLRDPKRIFGFQNVAPIVSVKTVQQQGSGFTTTIDRVSKTL
                     TTQSLRQMNAEVMLKKRPPAQVAQQFLREHDLA"
     gene            complement(7268..8827)
                     /locus_tag="VF752_17770"
     CDS             complement(7268..8827)
                     /locus_tag="VF752_17770"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8857445.1"
                     /translation="MFGRLLGLATLKRSITTRMLVVSGVLVLTFVAVFALLVHASVEQ
                     RGSARLAFRSQEAIASGNKLEKLVIDLETGVRGFVASGGRNTFLQPWTQARRQYPTEA
                     ATLAHLVRDHPDQARMAAEIKSAMDDYVNLYSLPMIALARERPEVAKSVIVNGTGKDR
                     VAGIRGEFSRLFTAERSLVNTRETRAENRSKTAIALGIAGLGFAIVAVILLGLYLARS
                     IVRPVRRVAAGAERVAGGDLSARVPDARADELGDLGRAFNTMAASLEESRDEIDRRTR
                     DLERSNRELAQYAAVTSHDLKEPLQTVSLFAQLFERRYKGRLDPQADQYIDSIISGTE
                     HMRTLIRDLLEYSRVGRGEVRRETVDGEVVFQKVLDNLDASISERGAEITHDPLPSVE
                     GDPNQIGQVLQNLISNGLKFNAEGAPRVHVSASRDSGFVLFSVADNGIGIDEEHAHKI
                     FEPFARVGERYEGTGIGLAICHKIVERHGGRIWVESRPGEGSTFKFTVPAASGRRSAP
                     AAAPEKAPTPA"
     gene            complement(9235..9307)
                     /locus_tag="VF752_17775"
     tRNA            complement(9235..9307)
                     /locus_tag="VF752_17775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9272..9274),aa:Lys,seq:ttt)
     gene            complement(9338..10927)
                     /gene="purH"
                     /locus_tag="VF752_17780"
     CDS             complement(9338..10927)
                     /gene="purH"
                     /locus_tag="VF752_17780"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX8857446.1"
                     /translation="MATAEPSATAVTGPGQVQIRRALLTVSDKRGVVDFARGLTELGV
                     EIVASGGTARELEEAGLKVRSVEDYTGFPEILDGRVKTLNPRIYAGLLAVRSNTEHVN
                     TLSEHDIEPIDLVCVNLYPFERVANARGVEDLEIIENIDIGGPTLIRAAAKNHAYAAV
                     VVKPESYDAVLEEMRTSDSRLSEQTRETLAMEAFSYTARYDAAISRWFAEKEESFPAL
                     YVRAFEKVLDLSYGENPHQRAAYYMQAGARTDLLSMVSKLHGKELSFNNLLDLDSGRF
                     LLDEFELPSTVIVKHNNPCGVAVGKSIGESFERALATDPLSAFGGIYCFNRPVDAALA
                     EELSKIFVEVVFAPGYEDDALEILTRKPNIRILYDQERRKVPFGEHDFKRVRGGLLIQ
                     DRDHESEAREDMTVATTVRPSEQQWGDLMFAWRVCKHVRSNAIVMAKDVATLGIGAGQ
                     MSRVDSVKLAIEKARSPLDGAVMASDAFFPFADGPEAAIEAGVKAIIQPGGSQRDPEV
                     IEACEKARVAMVFTGRRHFRH"
     gene            complement(10914..11546)
                     /gene="purN"
                     /locus_tag="VF752_17785"
     CDS             complement(10914..11546)
                     /gene="purN"
                     /locus_tag="VF752_17785"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX8857447.1"
                     /translation="MSFRVAVLVSGEGTNLQAILDKVHGHEGIEVVGVASSRSEARGL
                     ERARTAGVGTGVFAASDYQDRPARDAALADWLEGLGVDLVVLAGFMELLSPEFIRRFP
                     RRIINVHPSLLPAFPGIRSIEQALEHGVRVMGVTIHFVDEGVDTGPIVMQCSFDLSYP
                     ARIEDVEDRVHAIEHELLPQAVRLIAQGRVSFDESNPRRVLIEEEANGHS"
     gene            complement(11543..>12813)
                     /locus_tag="VF752_17790"
     CDS             complement(11543..>12813)
                     /locus_tag="VF752_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX8857448.1"
                     /translation="QAIVAATATATPQVSTDIVRRLRLSEPLRVATGFDRPNIAFGVA
                     RPAPHEKHGMLLGALGDPDALPAIVYAGTRAGAEDMASLIGRELGVDAAAYHAGLDRE
                     RRAAVQRAFLADEVKVVVATNAFGMGVDKPNVRSVVHASVPASLEAYYQEAGRAGRDG
                     LPARALLLAENRDKALHVHFIKRDELRPELGRALAERLVFAADGDGRYDVDAGELSRG
                     LGLNGDQLRALVGHLAQAGALQPMPSAADRVSGRLLAEFDRRAEALTRASMAEAGRAR
                     WRQYREMWAYVDSDACRRAALLAHFGDASPPSPSGSCCDSCEPALLDGLAPEPDQLAA
                     EVGTLDDAIISVVRSARPSVGRTTCAEILHGARTKKIERNSYDGLPAYATSSRMRRAD
                     ILARIDELIGERRLETTRGPYPVLQLPSAA"
BASE COUNT         2015 a         4446 c         4346 g         2006 t
ORIGIN      
        1 agcaggcgac ccgcgtcgcg cgccgacggt gacccgacca cggtgatgcc gagcagtccg
       61 tcgtcgagcc gcgggcgcgt gcccgaagcg accacccgtc ccagcctgta gcggttgttc
      121 gacacgagca ctgcggcgcc gccgctgtgg gcgtggccgc cagggcccgt ccagcggaga
      181 tcgagctcgg aaccatccgg ccccaacacg tcggggaccg tgtcgaggag cgtccggagc
      241 ttcgcgtcgc ggtagccgcc tcgctgcact gcctcggcgt acacgccgag cgacacgttg
      301 ttcacgaaca cgcgcccatt cacctccgcg aggtccacac ggcgctcacc gccgtcgacg
      361 aacgcgtcga gcgccccgac cacgtcgtcg cggtccaccc ccagatcgag cgcgaagtga
      421 ttgcgcgtgc ccgccggcac gcaggcgtac ggcagcccat ggtcagcagc gagagcggcg
      481 actattgcct gcgagccgtc gccgcccgcc atcgccaacg cgtcggcacc gccgtcaacg
      541 gcccggcgga cgagcgtctc caggttctcg cccggcgtca gctcgatcgg ctcgatccct
      601 cgcgcgcgcg cctgctcggc gagcgagaat cgctccgcct tgccgccgcc cgagcgcggg
      661 ttgaagaaca gcaccgggcg cttcggggcc gctgcggccg gtagcgcggc atggaccgcg
      721 atgctccgct cggaacccgc caccgagacg agcaccccac tcgcaaccag cagccacacg
      781 agcaaggacc cgccgaccag gaggagcgca atcacccccg ccagcgcgag gcctgcgacg
      841 accaggcccc cgacccgcgc aatcccgcgt ctgagcacgc cataccaggc ggccgcggcc
      901 gccagtgcga cgcaggccag caccgccagc ccgcgcggga acgcggtgat cacgacggcg
      961 atgccgaggc cgagcgtgat ggcacccacc acgagcacga gggccgctga agaccgtcgg
     1021 ctcataggat ccagccacct tgagcgaccg ccgagaaccg agctggatcg aggacgccaa
     1081 gcgcctggac ctcgccgtgt actccgccat cgcgagcacg ccgactccgg agctcgacgc
     1141 ggcgatgagc aggctctctc gcgccgccga ctactcgcgc ctgtcgctgg cgtcggcggc
     1201 actgctcgcg ctcgcgggag gacggcgcgg ccggcgcgcc gcggcgaagg ggctcgcgtc
     1261 gctcggcgtg acggccaccg tggtgaacgt cgtcgtgaag ccgctcggac gcaggcaccg
     1321 tcccgaccgt gtcggcagcg acgtgccgct cgctcgccac gtgccgatgc cgaccacgcg
     1381 ctcttttccg tcgggccact cggcgagcgc gttcgcgttc gcgaccggcg tcggccacgt
     1441 gatgccggct gcgggcgtgc cgctgcgcgc gcttgccgcg ctcgtcgcgt actccaggat
     1501 ccacacgggc gtgcactatc ccggcgacgt gctcgccggc gcgctcatgg gcaccgcgct
     1561 cgcgcaggtc acgacgaagc tgctcgaccg ctgacacgga gccaagcctc gcgcaccgcg
     1621 cctcgcgcgg catagtccgg atggggtgaa ccgtccggcg gtatcacccc gtttgggcga
     1681 tttcgtggcg acagcggtgg attacgttcc gcgatgaccc cgacgtcgtg agcccgccta
     1741 tcgcccctcg acgaggttcg acatgtccgc cgagcacaca gtagagacgg ttgcccaagg
     1801 agacggggcg cttgcgcggg agctcgacga gctagctcag cagtacggga accgcttcct
     1861 gctcgagccg gcgcccgaca accacctccc ggaaaatggc atgcgggccg tggacgcgat
     1921 gcgcctgctc ggtgaggagc tcgtgctcga cggcatcccg atgcgcaatc tggccacgtt
     1981 cgtgacgacc tggatggagc ctgaggcgca gcgcatcatc gcggagaacc tgtaccgcaa
     2041 ctacatcgac cacgctgaat atccacagac cgcggagatc gagcaccgct gcatccggat
     2101 gctcgccgac ctcttcaacg cgccggggga gaccaccggg acgcggaccc agggctcgtc
     2161 ggaggcgatc atgctcggcg cgctgtcgct gaagtggaag tggcgcgcgc ggcgcgaggc
     2221 ggccggtcag gacaccacac gcccgagctt ggtgttcggc ggagacgtgc acgtggtgtg
     2281 ggagaagttc tgccgctact tcgacgtcga gccgcgcatc atcccgctgc agcccgacaa
     2341 gtacacgatc ggccccgagg acgtgcaccc ctacgtcgac gagaacacga tcggcgtggc
     2401 cgcggtgctc ggcacgacct caccggccac gcggacgaca tcgtcgggat caacgatctg
     2461 ctcgtccggc tcaagcagga gcggggcctc caagtcccca tgcacgtcga cgcggcgagc
     2521 ggcggcttcg tctggccgtt tctctacccc cataccgagt gggacttccg cctcgagcag
     2581 gttcactcga tcaacgtctc gggccacaaa ttcggcctcg tgtacccggg gatcggctgg
     2641 ctgatcttcc gcgagaagag cgacctgccc gaggacctcg tcttctacga gaactacctg
     2701 ggcaagcgcg acgccacgtt cacgctgaac ttctcgaccg gcgcttcgat ggtgctggcc
     2761 cagtactaca acttcgtccg cttcggccac gacggctacc gctacatcat ggagacgatg
     2821 caggccaaca cgcgggcgct ggcacagcgc atcgcggcga tcggcgactt ccggctaatc
     2881 ggctcgggcg acgaggagca gctgccgctc gtggccttcc agctggaggg cgagcacaac
     2941 tacgacgagt tcgacgtggc gtcgcagctg tccgcagagc gaggctgggt ggtccccgcc
     3001 tacacgctcc cgcccaatgc tgatcacgtg aagatcatgc gcgcgctcgt gaagctgaca
     3061 ctgggacata cgctcgcgac gacactggcg gacgacatcg cggaggcctg cgccgtgctg
     3121 gcccagaagg gcggcctgca cgagcacgac cgtaaacgcg tcaagaccgg cacgggctac
     3181 tgacaggagg acacgatgtg tcgctggctc gcatattcgg gctcaccggt gctcatggag
     3241 gagctgctct acaagcccca gcactcactg atcgtgcaga gcctccattc acagctggga
     3301 gccgaggaga cgaacggcga cgggttcggt gtcggctggt acggcgagct gtccacgccg
     3361 gccgtctttc gcagcatcga gcccgcgtgg aacgatcgca acctgcgtga gctctcaggc
     3421 cacatcaggt cgggcgtggt gttcgcgcac atccgcgcct ccaccggttc gccggtgcag
     3481 cagaccaact gccacccgtt ccgtcacggc cgctggctgt ggatgcacaa cggcctgatc
     3541 cacgacttct tgaaggtcaa gcgcgacctc gtgctcgaga tcgacccgtc agtctatccg
     3601 gacatggagg gcacgacgga ttcggaggtc atcttccacc tggcgttgac gttcgggctc
     3661 gaggacgacc cgcccacggc ggtcgagcga gccgtcggcc tggtcgaggc ggtcggcgcg
     3721 cgccacggcg tcgagcatcc aatgcagggc accatggcga ccacgaacgg cgagacgctg
     3781 tgggcgtttc gctactcgag cgaggggaag tcccgttcgc tgttcttcag cacggacgtg
     3841 cgcacgctga aggcgcagca ccccgagatc ccggcgctgc acgagctctc cgacgaatcg
     3901 aggctcgtcg tctcggaact cctcggggac ctcgccggcg cctggcacga agtgccagag
     3961 tcgagctacg gcgtcgttgg tccgggccag gccgaacttc ggccgttcac gccacggccg
     4021 ccagagcacg cgagcaatcc gaaagtgggc ggtgcccggc tcgaacgagc gacctcctgc
     4081 ttgtaagtga gcgcgagcca gaggaaggac gcgacgcagg gcggcaactg ggcttcacgc
     4141 gcggcccaac gactaggatc cgccttcact cgatcggact gtggctcacc caatcgatag
     4201 gaggcccgat gatcggcgtg ctcgtgactt tcacgcagac cgacaagttc gatccctcaa
     4261 ctgtcgcgaa gattgccgac gaagaacgag gaacgttcga gggcatgccc ggccttcgcc
     4321 tcaagagctt cacgctcgac gatgacggca gggcaaggaa cttctacttg tgggatgacg
     4381 aggagaaagc ccgtgccttc ttcacggagg aggtcgtcga catggtgacg gggatttatg
     4441 gcgtcgcgcc caagatcgaa tacctcgacg tattggagtt cgtcgacaac tctggcacct
     4501 gattcgttcc gatcctctaa gcggccgggg gatcggaata gccgctcgga cgagaagcga
     4561 aagcgaagcc gatacccgtt tgtaccctcc cgcgccgccg aatccggtgc ctgatgcaaa
     4621 ctccctgcaa agcgaaaagt gggcggtgcc cggctcgaac gggcgacctc ctgcttgtaa
     4681 ggcaggcgct ctaccaactg agctaaccgc cccggccggc tgagtgtagc ccggcccgcg
     4741 cggcggcccg cacgctagct tacggcgcga tggcattcgt gctcacgctg atcggcgccg
     4801 cgctcgcggt cgggctggag atgctcgaag cactggcgat cgtgctcgcg gtcggcctcg
     4861 aacgccgctt ccgcgactcg ctgctcggga cggcgggcgc cgccgcagcc cttgcggcgc
     4921 tggcggtcgt gctcgggccg gtgctgctct cgcgcgtccc gctcgagccg ctgcgcgtgg
     4981 tgatcggtgc cttccttctg ctgttcggac tcgagtggct tcgcaagggc gtcctgcgcc
     5041 tggccgggag gcggtcgcgc tcggactcgt tcaaggagtt cgtggaggag cgcgaggcgc
     5101 tgcatgaact gccacctccg ccgcctggcg aggccgactg ggccgcccgc gcgatcgcct
     5161 tcaagggcgt gctgctcgag ggtgtcgagg tgatcctgat cgtcaccgcg cttggcgctc
     5221 gtccgggcgg tctcgcgccg gccctgatcg gcggcgtcgt tgcgatcgcc gcggtgatcg
     5281 gcatcggcct cgtcgcccac cggccgctca gtcgcctgcc cgagacccac ctcaagtaca
     5341 cggtgggcgt cgtgctctcg accttcggcg tgttcttcgc cgccgagggc ctgggcgtcg
     5401 agtggccggg ctcggacgcg gcgctcctct acatcgcgct cgcgttcgtg ctgctctcgc
     5461 agctccagat cgcggtcatc tcccagcgcg gccgcaccgc cgaggcggtg gcgtcgtgaa
     5521 gacgctccgc cagttgcgca agctcgtact cggcgagacc tggtcgctgc cgctcggcat
     5581 cgggatcgcg ctcggaatag cggcgctgat ccacgaggtg gcgggcgagt ggtgggagca
     5641 ggccggcggc ttcatcctgc tcgggctcgc cgcgctggcg ctctcgatat cgcttggcgg
     5701 cgtcctgatc gcgcatgcgt ctcaaggccg cagcgcgcgt cgtcgataag taggggcgag
     5761 gggaatcgaa cccccgatct ccggcttgaa aggccggcgt cctaaccgct agaccacgcc
     5821 cccgtgtctg tgagagtcta ggcgtgactg gacgcgtgag ccgtatcacg ttcggactcg
     5881 cggtcggcgg gaacggttgg ccccggtgcc cccgacagat ctcacggcac aaccgatgca
     5941 agctctcccc atcctgtggc tcgccacgcc tttcatcacc ggcgtgtgca tcgcgagctc
     6001 gcacgccgcc cgccgcttca ccggcaggca ctacgtcggg tgcctcttgg ccggctgggc
     6061 cggggtgacg ctgcttctgg ccgccgcgtt cctgctcgac gggccgatcg ggaccgcggc
     6121 gctgatcgcc ggcgggccgc tcgcgggcct cagcttctgg ctgccacgcc gcggcaacga
     6181 cgaggacggc ggggacgagc ctgcggacga gccgtcgccg acgccgcctg atggcgattg
     6241 ggaccgcttc ctctcggact tctggaccta cgtggaccac cgccgcaggc cgagctcacc
     6301 ggcaccgcgc gagcgcgtct ccgcgtagcg ctttccgctc aggccaggtc gtgctcgcgc
     6361 aggaactgct gcgctacctg agcgggaggg cgcttcttca gcatcacctc ggcgttcatc
     6421 tgccgtagcg actgcgtcgt aagcgtcttc gagacgcggt cgatcgtggt ggtgaagccg
     6481 ctgccctgct gctggaccgt cttcacgctc acgatcggcg ccacgttctg gaagccgaag
     6541 atccgcttcg ggtcgcgcag caccgtgtag cggctgccgt tgagctggcc gtccgtggtg
     6601 aagacgtcgg cgacatcgac cttgcccttg tcgagctcgc cgtactgctc accgatcttg
     6661 gatggcacga accggatgtt cgtgaggccg tatacctgct cgagccccac gagtccctcg
     6721 aagcgggtcc ggaactcggg tggggccgcg agccgcacgc ttccgctgac cttcgcgaga
     6781 tcggcgacgg tgcgcagctt ccagcgcttg gcgtaggaag gcttgaccgc gagcgcgtcg
     6841 gtgttcgagt agggagtggg cgcgagcaga gtgaagccgc ggccctgctc gaagccgtcc
     6901 gccaggcggt acgcctgcgc ggcgctcgat gggcggcgcg agcggtgggc gacctcggtg
     6961 acgaggaccc cgatgtactc ggggtacatg tcgatcgcgc ccgcctgcag cgtgcggtgg
     7021 atgatctccg aggcaccgat gtcctccttc acgaccacgc ggaagccgcg tgcgcgcagc
     7081 gcctgcgcgt agagctggcc gagcaggtac tgctcggcga agttcttcgt cccgagcgtg
     7141 acgaccggct tcggtttctt gttcgccgac tcgccgccgg agctggtgcc gccgcacccc
     7201 gccacggcaa gcagggcgat ggccgcggta accgtgaccg ccgcgcggag tcgaattcgc
     7261 gccgccacta cgccggtgtg ggggctttct ccggcgccgc cgccggcgcc gagcggcggc
     7321 ccgacgccgc cggcaccgtg aacttgaacg tgcttccctc gcccgggcgg ctctcgaccc
     7381 agatccggcc gccatgccgc tcgacgatct tgtggcagat cgccaggccg atcccagtgc
     7441 cctcgtagcg ctcgccgacc cgcgcgaacg gctcgaagat cttgtgcgcg tgctcctcgt
     7501 cgatcccaat gccgttgtcg gcgacggaga acagcacgaa gccgctgtca cgcgaggcgc
     7561 tcacgtggac gcgcggagct ccctcggcgt tgaacttgag gccgttcgag atgaggttct
     7621 gcaggacctg cccgatctgg ttggggtcgc cctcgaccga cggcagcggg tcgtgcgtga
     7681 tctcggcgcc gcgctcgctg atggaggcgt cgaggttgtc gagcaccttc tggaacacca
     7741 cctcgccatc gaccgtctcg cggcgaacct cgccgcggcc cacgcgtgag tactcgagca
     7801 ggtcacgaat gagcgtacgc atgtgctctg tgccggagat gatcgaatcg atgtactggt
     7861 cggcctgggg gtcgagccgg cccttgtatc gccgctcgaa caactgggcg aagagcgaaa
     7921 ccgtctggag cggctccttg aggtcatgtg acgtgacggc cgcgtactgc gccagctcgc
     7981 ggttcgagcg ctcgaggtcg cgggtgcgcc ggtcgatctc gtcgcggctc tcctcgagcg
     8041 acgccgccat ggtgttgaag gcgcggccga ggtcgccgag ctcgtcggcg cgcgcgtccg
     8101 gaacgcgcgc ggacaggtcg ccgcccgcca cccgctcggc gcccgcggcg acgcgccgca
     8161 ccggccgcac gatcgaacgc gcgaggtaga gcccgaggag gatcacggcc acgatcgcga
     8221 acccgaggcc ggcgatgccg agagcgatcg ccgtcttcga gcggttctcg gcgcgcgtct
     8281 cgcgcgtgtt cacgagcgaa cgttcggccg tgaacagtct gctgaactcg ccacgaatac
     8341 cggccacgcg gtccttgccc gtgccgttca cgatcacgct cttcgcgacc tctgggcgct
     8401 cacgcgcgag cgcgatcatc ggcagcgagt agaggttcac gtagtcgtcc atcgccgact
     8461 tgatctccgc ggccatgcgc gcctggtccg ggtggtcgcg caccagatgc gcgagcgtcg
     8521 cggcctcggt ggggtactgg cgccgcgctt gggtccatgg ctggaggaac gtgttgcgcc
     8581 cgccgcttgc gacgaagccc cgaacgccgg tctcgaggtc gatcacgagc ttttcgagct
     8641 tgttcccgga cgcgatcgcc tcctgcgagc ggaaggcgag ccgagccgag ccgcgctgct
     8701 caacgctcgc gtggacgagc agtgcaaaga ctgcgacaaa cgtgaggaca agaacgccgc
     8761 tcacgacgag catgcgggtg gtgatgctgc gcttcaaggt tgcgagcccg aggaggcggc
     8821 cgaacacctc ggaatgggtc tacgtgatgg tagcccggcc ccggtcgtct gggcgatacc
     8881 ccgtatcggg gacagaaatc ggacatgcag ttacatcaaa gagcgggacg cagcgggcgt
     8941 ccatttcttc cacgagagag cgatttgctc caacgaggag cgtcaagcgg aaggccaata
     9001 gacgctgcgg cgttgccgag cgggccgcgc ggtggcccgg gaggcgaaaa agcaagcagt
     9061 gaggaactgg gcttggagcg ctgacgagcc gcgaaaagcg gcagtgagga aggagcttgc
     9121 agcactagac gagccgcgaa aaccgaggag tgcggtgacc ggcgaccccg acgcgtgctt
     9181 tgcacgcgag cggggagccg ggtgccgcaa tccgaaggtt tgcagcggct cgagtgggcc
     9241 gcctgggact cgaacccaga acctcggggt taaaagcccc tcgctctgcc aattgagcta
     9301 acggccctta ccttgatgct agagccgacg cggtcgacta atgccggaag tgccggcggc
     9361 cggtgaagac catcgcgacc cgggccttct cgcacgcctc gatcacctcg gggtcgcgct
     9421 gtgagccgcc cggctggatg atcgctttca cgcctgcctc gatcgcggcc tcaggcccgt
     9481 ccgcgaacgg gaagaacgcg tcggacgcca tcaccgctcc gtcgagcggc gaacgcgcct
     9541 tctcgatagc gagcttcacc gagtcgactc ggctcatctg gccggcgccg atgcccagtg
     9601 tcgcaacgtc cttcgccatg acgatcgcgt tcgagcgcac gtgcttgcag acgcgccacg
     9661 cgaacatcaa gtcgccccac tgctgctcgc taggccggac ggtcgtcgcg accgtcatgt
     9721 cctcgcgcgc ctccgactcg tggtcgcgat cctggatcag caggccgccg cgcacgcgct
     9781 tgaagtcgtg ctcgccgaac ggcaccttgc ggcgctcctg gtcgtacagg atgcgaatgt
     9841 tcggcttgcg cgtgaggatt tcgagcgcgt cgtcttcgta gccaggcgca aacaccacct
     9901 cgacaaagat cttcgagagc tcctccgcga gggcggcgtc gaccggacgg ttgaagcagt
     9961 agatgccgcc gaaggcacta agcgggtcgg tggcgagcgc gcgctcgaac gactcgccga
    10021 tcgacttgcc gaccgccacg ccgcacgggt tgttgtgctt gacgatcacg gtgctcggca
    10081 gctcgaactc gtcgagcagg aagcggccgg agtcgaggtc cagcaggttg ttgaacgaca
    10141 gctccttgcc gtggagcttc gagaccatcg ataggagatc ggtgcgcgcg ccggcctgca
    10201 tgtagtaggc ggcacgctgg tgcgggttct cgccgtagga gaggtcgagc accttctcga
    10261 atgcgcgcac gtacagcgcc gggaacgact cctctttctc tgcgaaccag cgcgagatcg
    10321 cggcgtcgta gcgcgccgtg taggagaacg cttccatcgc cagcgtctcg cgggtctgct
    10381 cggacaggcg cgagtcggat gtgcgcatct cctcgagcac ggcgtcatag ctctctggct
    10441 tgacgacgac cgccgcgtac gcatgattct tcgccgccgc gcggatcaac gtcggtccgc
    10501 cgatgtcgat gttctcgatg atctcgaggt cctccacccc gcgcgcgttg gcgacgcgct
    10561 cgaacggata gaggttcacg caaacgaggt cgatcggctc gatgtcgtgc tcgctcagcg
    10621 tgttcacgtg ctcggtgttc gagcgcaccg cgagcaggcc cgcgtagatg cgcgggttga
    10681 gcgtcttcac ccggccgtcg aggatctccg ggaagccggt gtagtcctcg accgaccgaa
    10741 ccttgaggcc ggcctcctcc agctcccgcg ccgtgccgcc tgaggcgacg atctcgacac
    10801 caagctcggt gagcccgcgc gcgaagtcga cgactccccg cttgtccgac acggtgagga
    10861 gcgctcgccg aatctggacc tgaccaggcc cggtgactgc cgtcgcgctc ggttcagctg
    10921 tggccattcg cctcctcttc gatcagaacc cgtcgcggat tgctctcgtc gaagctgacc
    10981 cgaccctgcg cgatcagccg cactgcctgg ggcagcagct catgctcgat cgcgtgcacc
    11041 ctgtcctcga cgtcctcgat tcgcgcagga taggagaggt cgaaggagca ctgcatgacg
    11101 atcggaccgg tgtcgactcc ctcgtccacg aagtggatgg tgacccccat cacccgcaca
    11161 ccgtgctcga gcgcctgctc gatcgatcga atgccgggaa atgcaggcag aagcgacgga
    11221 tggacgttga tgatccgccg cgggaagcga cggatgaact ccggtgagag caactccatg
    11281 aagccggcca ggacgacgag gtcgaccccg agcccctcga gccagtcggc cagtgccgcg
    11341 tcgcgcgccg gacgatcctg atagtcgctc gccgcgaaca cccccgtgcc cactcccgcc
    11401 gtacgcgctc gctcgagccc ccgcgcctcc gaacggctcg acgccacgcc caccacctcg
    11461 attccctcgt ggccgtgaac cttgtcgagt atcgcctgga ggtttgtccc ctcgccggag
    11521 accagcaccg caacccggaa gctcacgcgg cgctcgggag ctgcagcacc ggatacggcc
    11581 ccctcgtggt ctcgaggcgc cgctcaccga tcagctcgtc aatgcgcgcg aggatgtccg
    11641 cgcgccgcat gcgcgacgag gtggcgtagg cgggcaaccc gtcgtaggag ttgcgctcga
    11701 tcttcttggt ccgcgcgccg tggaggatct cggcgcaggt ggtgcgcccg accgacggac
    11761 gcgcgctcct gacgacggag atgatcgcgt catcgagcgt gccgacctcg gcggcgagct
    11821 gatcgggctc gggcgccagg ccgtcgagca gcgcgggctc gcaggagtcg cagcaggagc
    11881 ccgacggcga cggcggactc gcgtcgccga agtgggcgag cagcgcggcg cggcggcagg
    11941 cgtccgagtc gacgtacgcc cacatctcgc ggtactggcg ccagcgtgcc cgtcccgcct
    12001 cggccatcga ggcgcgagtg agcgcctccg ccctccggtc gaattcggcc agcaggcggc
    12061 cgctgacgcg gtccgccgcc gaaggcatcg gctgcaatgc tcccgcctgg gcgagatggc
    12121 cgaccagcgc gcgtagctgg tcgccgttca gcccgagccc acgcgacagc tctccggcgt
    12181 cgacgtcgta tcgcccgtcg ccgtctgcag cgaacacgag gcgctcggcg agcgcgcggc
    12241 caagctccgg ccgtagctcg tcgcgcttga tgaagtgcac gtgcagcgcc ttgtcacggt
    12301 tctcagcgag gagcagcgcg cgcgccggca ggccgtcgcg cccggcccgc ccggcctcct
    12361 ggtagtacgc ctcgagcgag gccggaacgc tcgcgtgcac cacgcttcgc acgttcggct
    12421 tgtcgacgcc catgccgaac gcgttcgtcg cgacgaccac cttcacttcg tcggcgagga
    12481 aggctcgctg caccgcagct cgccgctcgc ggtcgaggcc cgcgtgatac gccgccgcgt
    12541 cgacgccgag ctcgcggcca atgagcgacg ccatgtcctc cgctcccgcg cgcgtgcccg
    12601 cgtagacgat cgccggcaga gcatcggggt cgccgagggc accgagcagc atgccgtgct
    12661 tctcgtgcgg cgccggccgc gctactccga acgcgatatt cgggcgatcg aagccggtcg
    12721 cgacgcggag cggttctgag agccgcaggc ggcgcacgat gtcggtcgac acctgcgggg
    12781 tagcggtcgc cgtggcggcc acgatcgcct gcg
//
LOCUS       DASVXS010000318         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_633163, whole genome shotgun sequence.
ACCESSION   DASVXS010000318 DASVXS010000000
VERSION     DASVXS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633163"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VF752_17795"
     CDS             <1..318
                     /locus_tag="VF752_17795"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8857449.1"
                     /translation="NINRVFDDHDVVMTPVLAYQPPLIGKWEGRHALKLVDSVGRWIP
                     FTTPWNLTGQPAASVPAGFDADGLPLAVQVVARPGDEATLFSLAAQLEAERPWAEKRP
                     PLG"
     gene            complement(556..1560)
                     /locus_tag="VF752_17800"
     CDS             complement(556..1560)
                     /locus_tag="VF752_17800"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX8857450.1"
                     /translation="MALSRRLALLVIAVIAVAAVPANAMAALPGNWNKVDQRRVVRAG
                     LMTKDADGFHGERPFSPAAMRASLAALGQRTQTPPVQISSAPISVSRFDKLVVEQLGL
                     SDLAVAVQTEAKRAGLRPPSRFGTEVVARQLGLRFNHPFPFGERLELYPSDRITRAEA
                     AYSFARILSFNGWEKQYARDVLGRFTLPQYGPKQLAALRLAVSKIGMPYIWGGETDWP
                     SPGQVHGGYDCSGLAWRVYKLSGNPAGRAIRGRTAAQQAGEIPKRSRVAFADIRPADL
                     LFFGSAGFRSRATEASVTHEGIALSKDFMIHASSQGVYVSPLFEEWRRNAFAWARRVL
                     "
     gene            1619..2044
                     /locus_tag="VF752_17805"
     CDS             1619..2044
                     /locus_tag="VF752_17805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX8857451.1"
                     /translation="MWAQREIRLPALPRGYHLVTREIESGIPELERLHVGIAHLFIRH
                     TSASLTLNENASPDVRRDFATWFDQAVPDGASYWTHTLEGDDDMPAHVKSSLLDVALT
                     LPVSNGRLALGTWQGVYLCEHRDHGGPRSVVVTLFGEER"
     gene            2102..3565
                     /locus_tag="VF752_17810"
     CDS             2102..3565
                     /locus_tag="VF752_17810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857452.1"
                     /translation="MRWDNLRVEIEEGHRLPGYREPAVIRRFDAPEALNIRFYEVFAK
                     SALNRVPEQSQVPFRWTINPYRGCTHACTYCLAGDTRVLMADGTTQEIQHLRAGDLVY
                     GSEERGTQRQLVPTEVKDQWSTTEPAYRVTLDDGTELVASGEHRFLTPSGWKHVTGDG
                     DRPHLTPGAEIVGAEEDDDLRVASIDPLREEMLLYDITTGTGDFIANGVVSHNCFARP
                     THKYLDFDAGRDFEREIVVKVNVPEVLRRELARPSWKGEHVALGTNTDPYQWVEGRYK
                     LMPGIWEAFRDFRNPCSVLTKSPLLLRDLPLLKQVAEVAPISASFSVPTLDEKAWRAS
                     EPHTPNPRARLEAIGELNRAGIPCGVLIAPLMPGINDAPEQVEPILEMAAEAGAVSVG
                     GIALHLRGEVRQVFFEWLESYRPDLVPRYRELYKRGAYAPKEERARLQALARAKHDTR
                     DPSLPGRRFLRDGGDPWSARAEKRDDRHPPPDQPALF"
BASE COUNT          607 a         1241 c         1274 g          554 t
ORIGIN      
        1 aacatcaacc gcgtcttcga cgaccacgac gttgtgatga cgccggtgct cgcctaccag
       61 ccgccgctga tcggcaagtg ggagggccgg cacgcgctga agctggtcga cagcgtgggc
      121 cgctggatcc cgttcacgac gccgtggaac ctcaccggcc agccggcagc atcggtgcca
      181 gcagggttcg acgcggacgg cctgccgctg gcggtgcagg tcgtggcgcg gccgggcgac
      241 gaggcgacgc tgttctcgct cgcggcgcag ctcgaggcgg agcggccgtg ggcggagaag
      301 cgcccgccgc tgggctgagc ggggggcgag cagtaacggc ccgcacgccg gcaccgggag
      361 cgaagcgtcg tcagttaggg gccatagtgg gcccaaagtg acgacggccg ggtgcacaga
      421 ggtgtcaaaa gtccccggat cgcaatcgtg ctccgcgctt cccgaccaac tgcgtactcg
      481 cggcgcggag agtgacccgc cgacgctcac ccgtcattgc tgctgtgcag agtgagccgc
      541 gcggcccttg gagccctaca gcacgcgccg tgcccaggcg aacgcgttgc gccgccactc
      601 ctcgaagagc ggcgacacgt acacgccctg cgacgaggcg tggatcatga agtccttcga
      661 cagcgcgatg ccctcgtgcg tgacactcgc ctcggtggcg cggctgcgga acccggctga
      721 gccgaagaac aacagatccg cggggcggat gtccgcgaac gccacgcgcg agcgcttcgg
      781 gatctcgccg gcctgctgag cggcggtgcg gccgcggatc gcgcggcccg ctgggttgcc
      841 ggagagcttg tacacgcgcc acgccaagcc ggagcagtcg tagccgccgt gcacctggcc
      901 ggggctcggc cagtcggtct cgccgcccca gatgtacggc ataccgatct tcgaaaccgc
      961 caggcgcagc gccgcgagct gcttcggccc gtactgcggg agcgtgaagc ggccgagcac
     1021 gtcgcgcgcg tactgcttct cccagccgtt gaacgacagg atgcgcgcga acgaatacgc
     1081 cgcctcggcg cgcgtgatgc ggtcggacgg gtagagctcg aggcgctcgc cgaacgggaa
     1141 cgggtggttg aaccgcaagc cgagttggcg agcgacgacc tcggtaccga agcgcgaagg
     1201 cggccgcagg ccggcgcgct ttgcctcggt ttgaacggcc acagcaaggt cggaaaggcc
     1261 gagctgctcg acgacgagct tgtcgaagcg cgacacgctg attggcgccg acgagatctg
     1321 caccggcgga gtctgcgtgc gctgtcccag cgcggcaagc gacgcgcgca tggccgccgg
     1381 actgaacggg cgctcgccgt ggaaaccgtc ggcgtccttc gtcatcaggc cggcacgcac
     1441 gacgcggcgc tggtcgactt tgttccagtt gcccgggagg gcggccatcg cgttcgcggg
     1501 gactgccgcg acggcgatga cggcaatgac gagaagtgcg agtcgccggg agagggccac
     1561 gtgttcttcc tcggaacacg cgcgcttccc tttagtcgcg cgagtagcgt tcagccggat
     1621 gtgggcccag agagagatcc ggctgccggc gcttccgcgc ggctaccacc tcgtgacgcg
     1681 cgagatcgag tccggcattc ccgagctcga gcgcctgcac gtgggcatcg cgcatctgtt
     1741 catccggcac acgtccgctt cgttgacgct gaacgagaac gcgagccctg acgtgcggcg
     1801 cgacttcgcc acctggttcg accaggccgt gcccgacggt gcgtcgtact ggacgcacac
     1861 gctcgagggc gatgacgaca tgccggcaca cgtgaagtcc tccctgctcg acgtcgcgct
     1921 cacgctcccg gtgagcaacg gccggctcgc gctcggcacg tggcagggcg tgtacctgtg
     1981 cgagcaccgc gaccacggcg gcccgcgaag cgtggtagtc acgctgttcg gcgaggagcg
     2041 ctgaggcgag cgtgcgttgt cgcccgcttc ccgtcgtctc gatatgcgaa catgtgttcg
     2101 tatgcgttgg gacaacctga gagtcgagat cgaggaggga caccgcctgc ccggctaccg
     2161 cgagccggcg gtgatccgcc ggttcgacgc gcccgaggct ctcaacatcc gcttctacga
     2221 ggtgttcgcg aagtcggcgc tgaaccgcgt gcccgagcag tcgcaggtgc cgttccgttg
     2281 gacgatcaat ccctaccgtg gctgcacgca cgcgtgcacg tactgcctgg ccggcgacac
     2341 gcgcgtgctc atggcggacg ggactaccca ggagatccag cacctgcgcg ccggcgacct
     2401 cgtgtacgga agcgaggagc gaggtacgca acggcagctg gttccgactg aggtcaagga
     2461 tcagtggtcg acgaccgagc cggcgtaccg ggtgaccctc gacgacggca ctgagctcgt
     2521 cgccagcggc gaacaccgct tcctgacacc gagtggctgg aagcacgtca cgggcgatgg
     2581 agaccggcct cacctgacac ccggtgccga gatcgtcggc gcagaagaag atgacgactt
     2641 gcgcgtagcg tcgatcgacc cacttcgaga ggagatgctc ctctacgaca tcactaccgg
     2701 gaccggcgac ttcatcgcca acggcgtcgt gagccacaac tgcttcgcgc gccccacgca
     2761 caaatacctc gacttcgacg caggccgcga cttcgagcgc gagatagtcg tgaaggtcaa
     2821 cgtgcccgag gtgctccggc gcgagcttgc tcgcccgtct tggaagggcg agcacgtcgc
     2881 gctcggcacg aacaccgatc cgtaccagtg ggttgaaggt cgctacaagc tcatgccggg
     2941 tatctgggag gcgtttcgtg acttccgcaa cccgtgctcg gtgctgacca agtcgccgct
     3001 cctcctgcgt gaccttcctc ttctcaagca ggtcgcggag gtcgctccga tcagcgcgag
     3061 cttctcggtg ccgacgctcg acgagaaggc ctggcgcgcg agcgagccgc acacacccaa
     3121 cccgcgagcg cggctcgagg cgattggcga gctgaaccga gcaggcatcc cctgcggcgt
     3181 gctgatcgcg ccgctcatgc cggggatcaa cgacgccccc gagcaagtcg agccgatcct
     3241 cgagatggct gctgaagcgg gagcggtcag cgtcggcgga atcgcgctgc acctgcgcgg
     3301 cgaggtccgg caggtgttct tcgagtggct cgagtcctat cggcccgacc tcgtcccgcg
     3361 ctaccgcgag ctctacaagc gaggtgcgta cgcgccaaag gaggagcgcg ctcgactcca
     3421 ggcgctcgcc cgcgcgaagc acgacacgcg cgatccctcg ctccccggtc gccgttttct
     3481 ccgggatggg ggcgatccct ggtccgcgcg cgccgagaag cgtgatgatc gtcacccgcc
     3541 gcctgaccaa cctgcgctgt tctgatgcgc aaggtttgag gtgcggttgg ggatggctat
     3601 gtcacctgtg ggacccgcac ccgaccttcc cccctcgtga gacggatttt ccgcagacca
     3661 agcgacgcgg acgcgc
//
LOCUS       DASVXS010000319         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_634081, whole genome shotgun sequence.
ACCESSION   DASVXS010000319 DASVXS010000000
VERSION     DASVXS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634081"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1456)
                     /gene="glyA"
                     /locus_tag="VF752_17815"
     CDS             complement(170..1456)
                     /gene="glyA"
                     /locus_tag="VF752_17815"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX8857453.1"
                     /translation="MTDLSPDYFTRPVADVDPEIAEVLENELRRQETTLEMIASENFV
                     PQSVLDCQGSVLTNKYAEGYPGRRYYGGCEYVDIAEQLAIDRAKEIFGAEHANVQPHA
                     GAQANASVYHAFLQPGDRILGMKLDHGGHLTHGMKINVSGRLYDIVPYGVRESDSWLD
                     MDALAELARESKPKLILAGWSAYPRQLDFARFREIADEVGAYLMVDMAHFAGLVAAGE
                     HPNPVPYADVVTTTIHKTLGGARGGMILSKEEHAKAINSAVFPGQQGGPLMHVIAGKA
                     VSLKIAQSETFRERQRRTRTGAAALAEELLAAGVNVLTDGTDVHLVLVDLRDSHLDGK
                     QAEDRLHDIGITVNRNAVPFDPRPPAVSSGLRVGTPALATRGLQVDDFHEIGKMIGEA
                     LVAEDWTDSMKSELSDRTRTLAEKYPLYPQLHPAAV"
     gene            complement(1529..2140)
                     /locus_tag="VF752_17820"
     CDS             complement(1529..2140)
                     /locus_tag="VF752_17820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX8857454.1"
                     /translation="MTPDDVRELERVLAAGGIALFPADTVYGLAVDPGSESAVARLYE
                     LKGRPPARPSAVMFFDRGAALEELGWLGPRTREAAEHLLPGPVTLVLPNPERRWPLAC
                     GPEPERVGVRVPALEGALEPLRAATGPVLQSSANPSGGPDARRLADVDERIRAGVDIE
                     LDGGELPGTPSTVIDLSTYEAEGSFDVLREGVLPKARVVELLA"
     gene            complement(2137..2934)
                     /gene="prmC"
                     /locus_tag="VF752_17825"
     CDS             complement(2137..2934)
                     /gene="prmC"
                     /locus_tag="VF752_17825"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX8857455.1"
                     /translation="MPGVREALDQAERAIARAGSDTARLDAELLAADALGITRAQIYA
                     DGRVQMPDAAARRLDALVARRVRREPVAYILGSKGFRHIEVAVDSRVLIPRPESELLV
                     ESALGLPDGAHAHDVGTGSGAIALALKQERPGLVVSASDVSPDAVAVARLNARQLGLD
                     VPVAVHRGLPPGQYDLVIANLPYVREDEWEGLAPEITEYEPREALVSGADGLDAIRAL
                     VREVPAGTALALEHAPAQAEAVRALLDDAETHRDLAGRERATIGRAR"
     gene            complement(2934..3989)
                     /gene="prfA"
                     /locus_tag="VF752_17830"
     CDS             complement(2934..3989)
                     /gene="prfA"
                     /locus_tag="VF752_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX8857456.1"
                     /translation="MIDKLVEQIESRFAELSQQMSDPSVIGDRDRYADVGRQYRALEP
                     AHQLAEEYRRAADDAAGARELLSEDGDDPELRELLEASQRRLEELEDEIRLAMVEPDP
                     NDDKNVIVEIRAGTGGEEAGLFAGDLYRMLTRYAERRGFKTEPLSISDGAYTFEIRGN
                     GAYSVFKYEGGTHRVQRVPETESQGRIHTSTATVAVLPEAEEVEVSIDPNDLQIDVYR
                     SSGPGGQSVNTTDSAVRITHKPTGLVVSMQDEKSQLQNRDKAMRVLRARLYEQEVAAQ
                     QAELSAERRAQVGTGERSEKIRTYNFPQDRVTDHRVKLTKNNLEAVLGGELDDFTAAL
                     QAEEKRRKLEAGALVES"
     gene            complement(4024..4500)
                     /locus_tag="VF752_17835"
     CDS             complement(4024..4500)
                     /locus_tag="VF752_17835"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8857457.1"
                     /translation="MRKFNVFSPAPEYDSSDPEGYRAGRDSFGAKIGAEQIGASVYEL
                     PPGQSVCPYHYEYPNEEWLMVLEGRPTLRHPEGEDELEPGDTVAFPPGPDGAHKVTNN
                     TDERARIIMFSTEVPPFVAVYPDSDKLLVKPHRYDSHDRLMVRRESSVDYYDGETP"
     gene            4559..>5269
                     /locus_tag="VF752_17840"
     CDS             4559..>5269
                     /locus_tag="VF752_17840"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8857458.1"
                     /translation="MNLPSAKAVGLSSVLVLGLAAPAAAGDSAPPPLEPALTINGAQP
                     VPVDKPVRLDASESKGDIRGFRFDLDGNGSYETDTGTTPYVDHAFSEPGRVRVRVLVT
                     DTAGRAQGAAQTIEVAAPKPVVVAAKPQAKAKQNPQAKAEQKPRSKATPQAHAGASSS
                     VTIKDFSFGPKSISIKAGDTVTWTNTGQAPHDASGSGFKIPVLTTGKSASHTFNTPGT
                     FSYICSIHPFMKGTVVVAG"
BASE COUNT          757 a         1863 c         1834 g          815 t
ORIGIN      
        1 ggcggacgac aggcgcgccg cgcaggttcc gagcgtacgc gtcggactcg tcccggccta
       61 cagctcgcgt tagccggcga aaaccgagct atgcaaggac gcagggccga agcttggcga
      121 tagccaagcg agcgcccgag gacgccgcag agcgaaggtt tgcagccgac taaaccgcag
      181 ctggatgaag ctgcgggtag agcgggtact tctcggccag cgtccgtgtc cggtcgctga
      241 gttctgactt catcgagtcg gtccagtctt cggcgacgag ggcctcgccg atcatcttgc
      301 cgatctcgtg gaagtcgtcc acctgcaggc cgcgggtggc gagcgccggt gtgccgaccc
      361 gcaggccgga cgacaccgcc ggcgggcgcg ggtcgaacgg gacagcgttg cggttcaccg
      421 tgatgccgat gtcgtgcaga cggtcctcgg cctgcttgcc gtcgaggtgc gagtcgcgca
      481 gatcgacgag caccaggtgc acgtcggtgc cgtccgtgag cacgttcact cccgcagcga
      541 gcagctcctc agcgagagcg gcggcacccg tgcgggtgcg gcgctggcgc tcgcggaagg
      601 tctcggactg cgcgatcttc agcgacacgg ccttgcccgc gatcacgtgc atgagcgggc
      661 cgccctgctg accggggaag acggccgagt tgatcgcctt cgcatgctcc tccttggaga
      721 ggatcatgcc gccgcgcgcg ccgccgagcg tcttgtggat cgtggtggtg acaacgtctg
      781 cgtagggcac cgggttcggg tgctcgcccg ccgcgacgag ccccgcgaag tgcgccatgt
      841 ccaccatcag gtaggcgccc acctcgtcgg cgatctcgcg gaagcgcgcg aagtcgagct
      901 ggcgcgggta cgcggaccag ccggcgagga tcagctttgg cttgctctcg cgcgccagct
      961 cggcgagggc gtccatgtcg agccacgagt ccgactcacg gacaccgtag gggacgatgt
     1021 cgtagaggcg ccccgagacg ttgatcttca tcccgtgcgt gaggtggccg ccatggtcga
     1081 gcttcatgcc gaggatccga tcgcccggct gaagaaacgc gtggtagacc gacgcgttcg
     1141 cctgcgcgcc tgcgtgcggc tgcacgttcg cgtgctcggc gccgaagatc tccttcgcgc
     1201 gatcgatcgc gagctgctcg gcgatgtcca cgtactcgca gccgccgtag tagcgcctgc
     1261 cggggtagcc ctccgcgtac ttgttggtga gcaccgagcc ctggcagtcg agcaccgact
     1321 gcggcacgaa gttctccgac gcgatcatct cgagcgtcgt ctcctggcgc cgaagctcgt
     1381 tctcgagcac ctcggcgatc tccgggtcga cgtcggcgac cggtcgcgtg aagtagtcgg
     1441 ggctgaggtc cgtcacgtgg gctccgttct cggctggtct gcggggcatg tccaccgtac
     1501 cagcgaggcc ggagccacag cccggcggtc acgccagcag ttcgaccacc cgcgccttcg
     1561 gaaggacccc ctcgcgcagc acgtcgaagc tgccctcggc ctcgtacgtc gacaggtcga
     1621 tcacggtcga aggggtgccc ggcagctcgc cgccgtccaa ctcgatgtcc accccggcgc
     1681 ggatgcgctc gtcgacgtcg gccaggcggc gcgcgtccgg accgccgctc gggttggcgc
     1741 tcgactgcag caccggcccg gtggccgccc gcagcggctc gagtgcgccc tcgagcgccg
     1801 gtacgcggac gccgacccgc tcgggctcgg gcccgcacgc gagcggccag cgacgctcgg
     1861 ggttcgggag caccagcgtg accggcccgg gcagcaggtg ctcggcggcc tcgcgtgtgc
     1921 gcgggccgag ccagccgagc tcctcgaggg cggcgccgcg atcgaagaac atcaccgccg
     1981 acggccgtgc aggcggccgg cccttcagct cgtagagacg cgcgaccgcc gactccgagc
     2041 ccgggtcgac cgccagcccg tagaccgtgt cggcagggaa gagcgcgatg ccgcccgcag
     2101 cgagcactcg ctcgagctca cgcacgtcgt cgggtgtcaa cgcgcgcgcc ctatcgtcgc
     2161 ccgctcgcgg ccggcgaggt cgcggtgggt ctcagcatcg tcgagcagcg ctcgcaccgc
     2221 ctccgcctgc gcgggcgcat gctcgagcgc gagggccgta cccgcgggca cctcccgcac
     2281 caatgctcgg attgcgtcga gaccgtcggc gcccgatacg agcgcctcgc gcggctcgta
     2341 ctcggtgatc tcaggcgcca acccctccca ctcgtcctcc cgcacgtacg gcaggttcgc
     2401 gatcaccaag tcgtactgcc ccggcggtag cccgcggtgc acggcgaccg gcacgtcgag
     2461 cccgagctgg cgcgcgttga gccgcgcgac cgcaaccgcg tccggcgaga cgtccgacgc
     2521 actcaccacc aggccgggac gctcctgctt gagggcgagg gcgatcgcgc ccgagccggt
     2581 cccgacgtcg tgcgcgtggg cgccgtcggg gagcccgagc gccgactcga ccagcagctc
     2641 gctctcgggg cgcgggatca gtacgcgcga gtcgaccgca acttcgatgt ggcggaagcc
     2701 cttgctgccg aggatgtacg ccacgggctc gcgccgcaca cgccgcgcga cgagcgcgtc
     2761 gaggcgccgc gccgccgcgt ccggcatctg gacgcgcccg tccgcgtaga tctgggcgcg
     2821 cgtgatcccg agggcatctg cggcgagcag ctcggcgtcg agccgcgcag tatcggagcc
     2881 cgcgcgcgcg atcgcgcgct ccgcttggtc gagcgcctcg cggacgcccg gcatcaggac
     2941 tcgacgagcg ccccggcctc gagcttgcgc cgcttctcct cggcctgcag cgccgcggtg
     3001 aagtcgtcga gctcgccgcc gaggacggcc tcgaggttgt tcttcgtgag cttcacgcgg
     3061 tggtcggtca cgcggtcctg cgggaagttg taggtgcgga tcttctccga gcgctcgccg
     3121 gtgccgacct gcgcacgccg ctcggccgaa agctcggcct gctgtgcggc cacctcctgc
     3181 tcgtacagcc gcgcgcgcag cacgcgcatc gccttgtcgc gattctggag ctgcgacttc
     3241 tcgtcctgca tggacaccac gagcccggtg ggcttgtgcg tgatgcgcac cgccgagtcc
     3301 gtcgtgttca ccgactggcc gccgggaccg gacgagcggt agacgtcgat ctggaggtcg
     3361 ttcgggtcga tgctcacctc gacctcctcc gcctccggca gcacggccac cgtggccgtc
     3421 gaggtgtgga tgcgcccctg cgactcggtc tccggcaccc gctgcacgcg gtgcgtgccg
     3481 ccctcgtact tgaacacgct gtaggcgccg ttgccgcgga tctcgaacgt gtacgcgccg
     3541 tcggagatcg acagcggctc ggtcttgaaa ccgcggcgct cggcgtagcg cgtgagcatc
     3601 cgatagaggt cgcccgcgaa cagtccggcc tcttcgccgc cggtgccggc acggatctcc
     3661 acgatcacgt tcttgtcatc gttcgggtcg ggttccacca tggcgagccg gatctcgtcc
     3721 tcgagctcct cgaggcggcg ctgcgaagcc tcgagcagct cgcgcagctc ggggtcgtca
     3781 ccgtcctcgg acagcagctc gcgcgcgccc gcggcgtcgt ccgcggctcg gcggtactcc
     3841 tcggcgagct ggtgcgccgg ctcgagcgcg cggtattgac gcccgacgtc cgcgtagcgg
     3901 tcgcggtccc cgatcactga ggggtccgac atctgctgcg agagctccgc aaagcggctc
     3961 tcgatctgct cgacgagctt gtcgatcatg gccatttgag ggtagccggg ggcgcggtcc
     4021 gcgctagggc gtctcgccgt cgtagtagtc gacgctcgat tcgcgcctca ccatcagccg
     4081 gtcatgcgag tcataccggt gtggcttcac cagcagcttg tcgctgtccg ggtacaccgc
     4141 cacgaagggc ggcacctcgg tggagaacat gatgatgcga gcgcgctcgt cggtgttgtt
     4201 cgtgaccttg tgcgccccgt cgggcccggg cggaaaagcc acggtgtcgc cgggctcgag
     4261 ctcgtcctcg ccctcggggt ggcgaagcgt cgggcggccc tcgagcacca tcagccactc
     4321 ctcgtttgga tactcgtagt ggtacgggca gacgctctgg ccaggcggca actcgtacac
     4381 cgaggcgccg atctgctcgg ctccgatctt ggcgccgaag ctgtccctac cggccctgta
     4441 gccctccgga tcggacgagt cgtactccgg ggctggtgag aagacgttga atttgcgcat
     4501 gggctgcgag aaggtagccg ggaacggatt tacgcgtcgg cggcgtccta cttccatgat
     4561 gaaccttccc tctgccaagg cagtcgggct ctcctccgtg ctcgtgctcg ggctcgctgc
     4621 cccggccgcc gcgggtgaca gcgcgccgcc gccgcttgag ccggcgctga ccatcaacgg
     4681 ggcgcagccc gtcccagtcg acaagccggt gcgtctcgac gcctccgagt cgaagggcga
     4741 catccgcggc ttccgcttcg acctcgacgg caacggcagc tacgagaccg ataccggcac
     4801 gacgccttac gtggaccacg cattcagcga gccgggtcga gtgcgcgtgc gcgtgctcgt
     4861 gacggacaca gccgggcgcg cgcagggtgc ggcgcagacg atcgaggtgg ccgcgccaaa
     4921 gcccgtggtg gtcgccgcga agccccaggc gaaggcgaag cagaacccgc aggcaaaggc
     4981 cgagcagaag ccgcggtcga aggcgacgcc tcaggcgcat gcgggcgcct ccagctccgt
     5041 gacgatcaag gacttcagct tcggtccgaa gtcgatcagc atcaaggcag gcgacacggt
     5101 gacgtggacc aacaccggcc aagcgccgca tgacgccagt ggcagcggct tcaagatccc
     5161 cgtgctcacg accggaaaga gcgcgtccca caccttcaac acacccggga cgttcagcta
     5221 catctgctcg atccacccgt tcatgaaggg caccgtcgtg gtggccggg
//
LOCUS       DASVXS010000320        16039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermoleophilaceae bacterium isolate SMAG_U2581
            k141_636092, whole genome shotgun sequence.
ACCESSION   DASVXS010000320 DASVXS010000000
VERSION     DASVXS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960235
            Sequence Read Archive: SRR10127268
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoleophilaceae bacterium (soil metagenome)
  ORGANISM  Thermoleophilaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Thermoleophilales;
            Thermoleophilaceae.
REFERENCE   1  (bases 1 to 16039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 03:40:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,585
            CDSs (total)                      :: 3,536
            Genes (coding)                    :: 3,518
            CDSs (with protein)               :: 3,518
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 45
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 15 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16039
                     /organism="Thermoleophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636092"
                     /isolate="SMAG_U2581"
                     /isolation_source="soil"
                     /db_xref="taxon:2732252"
                     /environmental_sample
                     /country="Mexico"
                     /lat_lon="21.053 N 103.902 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VF752_17845"
     CDS             <1..1475
                     /locus_tag="VF752_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857459.1"
                     /translation="SAASFSNPFKMDPTDAKHLMAPGREVFETVYGPDTTSELDGSKD
                     WKQVFDLGTRKSPGTGASTSGSDDSDNKASALDLKGDKAYVAYCGFCDVMTEGRPFES
                     GIATNVGGSAPAKRMTSDGWHIAAANGLPERYITGVAMDPANPNTVYVTLGGYGRHWA
                     PPGAVREDTSKVGTGHVFRSTDAGEHFTDVTGNLPDVSADSIVVRGGQLVVGTDIGTF
                     ISNDPAGSTWAVLGSGLPSVPVFHLSLKPGDDNTLVAATYGRGVYTYKFADAAAAAAS
                     GGNGKGSVCAATAGFRGFSATYKGGNLRVGISRRSPAGATVEVFRHSLGERRAIRKTL
                     VKRFRNRKRSFDYRKRLADGIYSVRAGIAVPGSHVRDYRSRTFEVKHGRYHGMRDFYG
                     HGSCGTIRSFKLSSPAFGGSTQRGLQIAYRLARSGTVTVTVKRARTITTRFKAMKARA
                     HRTYRLNLAARGLRRGTYSVTVTARSGRSATSQSLASTRL"
     gene            1561..2433
                     /locus_tag="VF752_17850"
     CDS             1561..2433
                     /locus_tag="VF752_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857460.1"
                     /translation="MARAKSSSSSRNSRSKSGSSRSNSRSSRSSSGNGKSSSNANRSK
                     EKLPDYSVIDPDQIEQAVDVYVDVPVVNVDEIKFELDDLRAHVAVLAEVGHFVQLNAG
                     VAARLGNVELDIKGVETQALLEARLHNVTKILGRVFTSLDRNPELLESVGQALGDVGG
                     GAHDLISDTGDVVKSVGKGGESAVQDIGKGAGRGVEGIGRGAEQGVQGLGQGAQQGVE
                     GLGQGAAQGVEGLGQGAAQGVEGLGQGAQQGVEGLGQGAGQGVEGLGQGAEQGVEGVG
                     QGVQQGVGNLAQQG"
     gene            2570..3916
                     /locus_tag="VF752_17855"
     CDS             2570..3916
                     /locus_tag="VF752_17855"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX8857461.1"
                     /translation="MSQADREYRKYLFEEERDRTGPPDVVLDVPELQVDLIHFELDNL
                     DAHVALKAKVLNLVKLNVGADVHLSRVKLDIKGVHAEVVLKARLDHVAAIVDRLMTTL
                     DRNPDLIKGLSQAVSEVGAGAGEAVDKTGDAAKDIGQGAQGALKDVGKGAGQAVGDVG
                     EGAGQAVGDIGEGAGHAVGDVGQGAGQAVGDVGQGAGQAVGDVGQGAGQAVGDVGQGA
                     GQAVGDVGQGAGQAVGDAGQGAGQAAGNLGGLVGGVGQTVGQAGQGATEALGGVTQGL
                     GGQQGQQGQQGQQQQPAREGQPDQQGQQQPAQQGQPDQQGQPDQQGQPDQQGQQAQPA
                     QQDQPQGAQEETAQTNGGGPNAAKPSSLAKEVAKMVARQIGQAAQDEARDLGFAATRK
                     VKELGERREQRRAEKYHATEAAMRLADELGVDLAWCDGTGSDGKITVKDVREAVEA"
     gene            3913..4158
                     /locus_tag="VF752_17860"
     CDS             3913..4158
                     /locus_tag="VF752_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857462.1"
                     /translation="MSRRKREPDVQIAASIKADELRFEVKPDVEVRAYSNSDAEAESV
                     SERENLPDELEPGVTYRDFAVRWRLAARLKDPDEDEG"
     gene            complement(4176..4631)
                     /locus_tag="VF752_17865"
     CDS             complement(4176..4631)
                     /locus_tag="VF752_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857463.1"
                     /translation="MAETTRRSSSKSRSSRSSSNGGSGNASTPNRRSSGSSASRSRSS
                     RSSSASSRQNASASSAQQRNGGGGVAGTIKNLAVPAVTAAAGIVGGVVLERRHKRPRK
                     VLGVPIPGTGSGMNGLTKEVRKAGKQFGKLASEVQTTRKKAEDIGNALS"
     gene            complement(5054..5590)
                     /locus_tag="VF752_17870"
     CDS             complement(5054..5590)
                     /locus_tag="VF752_17870"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8857464.1"
                     /translation="MTRRGTISSLGAAAAVALVALAAAGCGGGGGGAATASSPPTTAS
                     GHAATVSGANEGSLGKILVDSRGHTLYLFKKDSGAKSACFGACATNWPPLRSSRKPTV
                     GAGASASKVGTIRRSDGKPQVTYNRHPLYRFVGDKKPGDTNGQGLTAFGGSWFALTAA
                     GNQVSGQGSAGSSGGLGY"
     gene            5881..8655
                     /locus_tag="VF752_17875"
     CDS             5881..8655
                     /locus_tag="VF752_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8857465.1"
                     /translation="MLLGRQRECETLHGVLDAVRGGESRSLLLRGEPGVGKTALLEYA
                     IESAPDFRIARAVGVESEMELAFAALHQLCAPMLGRLDALPVPQRHALRVAFGLTVGD
                     APDRFFVGLAALSLLSAAAEEQPLLCVVDDAQWLDRASAEALAFVARRLLAESVAVVF
                     ATRTTSEDWRGVSDLVVEGLRDGDARELLGSVLRGPVDERVRERIVAETRGNPLALLE
                     LPRGLTPAELAGGFGLPDALPLSGRIEESFRRRLEELSAESRLLLIVAAAEPLGEPVL
                     VWRAAARLGIAADAAAPASAAGVLETGARARFRHPLARSAVYKAASPGERQRAHRALA
                     EATDPEVDPERRAWHRAHATTRPDEDVAAELERSAGRAQARGGLAAAAAFLECATALT
                     PEPARRVERALAAAQAKSQAGAPDAARELLSAAEAGPLDALQRVRADRLHAQIAFALN
                     RGSDAPPLLLKAAERLDRLDVRLARETYLEALTAAVFAHRLATAGGVLEVAEAAQAAP
                     PSSEPPRAPDLLLDGWVLLITKGRPTATPTLKRALSAFRSESISKEEEIRWLWLACHT
                     AFELWDDETWHVLSARLVDLARLAGALTELPVALDSLSVANLFAGRFAVAGSLLDEAK
                     AITEVTGSHPTPYGPVVFAACQGREADASELIEASTKEVVRRGEGMGLTVIRWASALL
                     YNGLGRYDDALAAAQLASAHPFDPTRGLPELIEAAARSGLPERASDALQRLAETTHSS
                     GSEWGLGVEACSRAVLSEGAVAENLYREAIDRLGRTRVRLALARAHLLYGEWLRRERR
                     RQQAREQLRTAHEMFTAMGIGAFAERAARELRATGETARKRTIETSDQLTAQEIQIAR
                     LARDGLSNPEIGSQLFISPRTVQYHLSKVFSKLAISSRAQLDDVLSSDRDLVGVSQRA
                     EEVAIRRPEL"
     gene            complement(8609..9301)
                     /locus_tag="VF752_17880"
     CDS             complement(8609..9301)
                     /locus_tag="VF752_17880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8857466.1"
                     /translation="MRVLIAYGQSLVRAGFRMLLESADGIRVVGEATSGEEAVALAGR
                     IRPDVALIDAQLSGIGSVEATRRMLSESSVAVMLLTASEDDERIFGALRAGASGLLLK
                     DIEPPELVRAVKLVADGEAALSPSLARRVIAELAARQEPSCPSSNLVDELTAREREVV
                     VLVALGLSNDEIAERLVVSRATAKTHVSRAMLKLAAHDRAKLVVFAYETGLVVPRAKA
                     PVAEWQPLPLAG"
     gene            9577..11154
                     /locus_tag="VF752_17885"
     CDS             9577..11154
                     /locus_tag="VF752_17885"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8857467.1"
                     /translation="MISSVTAQGARPVKILAVLAGALLAIAALAGTAHASGPGQPPPE
                     WAANAGEWPAHNYDLANTRATAQSPIDSQTVSRLKVKWTFPLSGSGAFGAFSSTPIAL
                     NGTVYFQDLNSNVFALDRSTGVRKWEHKFNRPSAGPNGIAFGNGRIYGATETSAFALD
                     ARNGQLVWSRKLIRNNREGIDMTPQLYDGKVLISTIPGNASVFYKGGALGVVWSLDAT
                     TGKPKWKFNTISDGAKLFGNTKVNSGGGLWYPPAVDDQGRVFISVANPAPLYGTKQFP
                     NGSSRPGPDLYTSSLVALDGQTGKRLWFRQAVPHDIRDYDLQIPAITTTVQLGGVPTE
                     VVLVAGKMGKVYAYRAADGKALWTRSVGKHQNDTGPLPRKLVTVFPGDLGGVETPMAL
                     AAGRLFVPWLDFPVRARAEGLPGGLAGTLKNFKVGRGGFTAFDAATGNVLWQRKLPTM
                     DFGAATVANDVVFTSDYAGTVYAFDAQTGKTLWTAKAPAGINSFPAIDGDTLLVGAGT
                     ASFDKKPKFELVAYSLQ"
     gene            11179..11553
                     /locus_tag="VF752_17890"
     CDS             11179..11553
                     /locus_tag="VF752_17890"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8857468.1"
                     /translation="MRRLAKRSTLAFVVAAIAALALVQLALGAAGPRASSTTDQVRGK
                     EFSFKLSTRSSRRPGKVRFTFRNVGTVQHDFRINGKQTPLIRPGKSASIVVNFKKPGT
                     YRYICTVPGHAAAGMRGVFTVR"
     gene            11599..12003
                     /locus_tag="VF752_17895"
     CDS             11599..12003
                     /locus_tag="VF752_17895"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8857469.1"
                     /translation="MLKEGRVATRLPAQDLNRAKAFYADKLGLHPAEEREGGLRYVCA
                     GGEFALFESAGAPSGEHTQMGWEVDDIDATVAELRSHGVVFEEYHAPGLETVDGIADI
                     RGNYPSKGAGERAAWFRDSEGNMLGIGQPVHA"
     gene            12040..12360
                     /locus_tag="VF752_17900"
     CDS             12040..12360
                     /locus_tag="VF752_17900"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEX8857470.1"
                     /translation="MIITTMNDLPGYQIDEVIGEVFGLTVRSRNIGSQIGASFKSLVG
                     GELKGMTKMLAEGRAHAQDRLVEEAEGKGANAVIAFRFDTSELGTTWTEICAYGTAVR
                     AHKL"
     gene            12357..13256
                     /gene="gluQRS"
                     /locus_tag="VF752_17905"
     CDS             12357..13256
                     /gene="gluQRS"
                     /locus_tag="VF752_17905"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX8857471.1"
                     /translation="MNGRFAPSPTGELHLGNLRTALLAWLFARSAGSRFLMRVEDLDP
                     QRSKPEHEERQLADLRAIGLDWDGDVVHQSERTELYRDALARLRTQGRVYECWCTRAE
                     IREAVQAPHGPLPEGAYPGTCRDLTEDEREERRRSGRPAALRLDARGDAVHFHDRLHG
                     AVIGTVDDLVLWRGDGTPAYNLAVVADDADQGVEEVVRGDDLLDSTPRQLLLARLLGI
                     AAPQYAHVPLVLAPDGSRLAKRHGAVTLAAIGPEDALSWMAASLGMAAPGERVTAADL
                     LERFDPDGLPLEPTVFSPSAAAR"
     gene            complement(13231..14145)
                     /locus_tag="VF752_17910"
     CDS             complement(13231..14145)
                     /locus_tag="VF752_17910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX8857472.1"
                     /translation="MTTDVYEDLRPLMFSIAYRMLGSVSEAEDVVQEAFLRLHRERGQ
                     VESPKAWLSTTTTRLAIDQLRSARARRETYVGEWLPEPLLTSGDETSRHAEVADSLST
                     AFLVLLESLSPVERAVFLLREVFDYPYDQIAAIVDKSQDNVRQLAVRARRHVDERRLR
                     FDASREERDELARKFFAAAEEGDLEGLEALLAEDVVLHGDGGGKAPALARPIHGRERV
                     AKVLLGWVRQMAGFDLDMRIVEVNGQPGAMTLDTEGAIVNVMSLDVAEGRIQGINSIV
                     NPDKLDHLGPVADVRALLRSVSERRRSG"
     gene            complement(14142..15107)
                     /locus_tag="VF752_17915"
     CDS             complement(14142..15107)
                     /locus_tag="VF752_17915"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8857473.1"
                     /translation="MQTKEDDMKVFIAGAGGALGKQLVPMLVTRGHEVTGTTTSQAKR
                     DLMRSLGAKPVVLDLLDARAVREAVLNASPDVVVHEATALSGSLDLRHFDRDFAMTNR
                     LRTEGTDNLLAAALASSAKRFVAQSFAGWPSERNGSAVKGEDAPLDPNPSPKFKTTLD
                     AIKHLESVVTGAEGIEGIALRYGGFYGPGTSLSRHGDYTEAIRKRQFPIVGSGSGIWS
                     FIHIEDAAAATVEAIEHGRRGIYNVVDDEPARVSEWLPVAAKAAGAKPPRHFPLWLGR
                     VLAGDAVVVMMTEVRGASNEKAKRELGWQPRHSSWRTTFPEVLAA"
     gene            15156..15572
                     /locus_tag="VF752_17920"
     CDS             15156..15572
                     /locus_tag="VF752_17920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX8857474.1"
                     /translation="MAELPPEVRALLEAPNFVHLATVNRDGSPSSVAVWGMLEGGRLV
                     FFTQRQTQKARNLERDARVAASIIDQEDPYKTARLRGRVVEKRTGQEAADTIDRISRG
                     YIGEAFPFHTPNEVVFAIEIEHTSFVELPFEHTPRS"
     gene            15574..>16039
                     /locus_tag="VF752_17925"
     CDS             15574..>16039
                     /locus_tag="VF752_17925"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX8857475.1"
                     /translation="MHHPISYFQAIILGLVQGAAEPFPVSSLGHGVILPRLLHWNIHQ
                     NDKFFLTFLVATHLATAIVLFGFFFKDWMLILGGMGRSLRDRQISEGDTYARLGWLLV
                     VGTIPAGVIGLLLEHPLRKLFASPQSAAAFLIVNGLLLFAFEKLRRRPPRPGD"
BASE COUNT         2559 a         5509 c         5590 g         2381 t
ORIGIN      
        1 tcagcgccgc gagcttctcg aacccgttca agatggaccc gaccgacgcc aagcacctga
       61 tggcaccggg gcgcgaagtg ttcgagaccg tctacggccc ggacaccacc tccgagctcg
      121 acggctcgaa ggactggaag caggtgttcg acctcggcac gcgcaagagc cctggcacag
      181 gcgcgagcac gagcggctcg gacgactccg acaacaaggc gtcggcgctc gacctgaagg
      241 gcgacaaggc gtacgtcgcc tactgcggct tctgcgacgt catgaccgag ggccgtccgt
      301 tcgagagcgg catcgccacg aacgtcggcg gctcggcgcc cgcgaagcga atgacctcgg
      361 acggctggca catcgccgcg gcgaacgggc tgccggagcg ctacatcacg ggcgtcgcga
      421 tggacccggc caacccgaac accgtgtacg tgacgctcgg tggctacggc cgccactggg
      481 cgccgccggg cgcggtgcgc gaggacacga gcaaggtcgg caccgggcac gtgttccgct
      541 ccaccgacgc cggcgagcac ttcacggacg tgaccggcaa cctgccggac gtgtccgccg
      601 actcgatcgt ggtgcgcgga ggccagctgg tcgtcggcac ggacatcggc acgttcatct
      661 cgaacgaccc ggccggctcc acgtgggcag tgctcggctc ggggctcccg agcgtgcccg
      721 tgttccacct gtcgctgaag cccggcgacg acaacacgct cgtcgcggcc acatacgggc
      781 gcggcgtcta cacctacaag ttcgccgacg cggcggcagc ggcagcgtcc ggcggcaacg
      841 gcaagggctc ggtgtgcgcc gcgaccgcgg gcttccgcgg cttcagcgcg acgtacaagg
      901 gcggcaacct tcgggtcggc atctcccgcc ggtcacccgc cggagcgacg gtcgaagtgt
      961 tccgccactc gctcggcgag cgccgcgcga tccggaagac gctggtgaag cgcttccgca
     1021 accgcaagcg ctcgttcgac tacaggaagc gcctggccga cgggatctac agcgtgcgtg
     1081 cgggcatcgc agtgccgggc agccacgtgc gcgactaccg ctctcgcacg ttcgaggtca
     1141 agcacggccg ctaccacggc atgcgcgact tctacggcca cggctcatgc ggaacgatcc
     1201 gctcattcaa gctctcgagt ccggcattcg gcggctccac gcagcgtggg ctgcagatcg
     1261 cctaccggct cgcgaggtcg ggcaccgtga cggtcacggt gaagcgcgcg cgcaccatca
     1321 ccacgcggtt caaggcgatg aaggcacgag cgcaccggac ctaccgcctg aacctcgcgg
     1381 cacgcgggct ccggcgcggc acctacagcg tcacggtcac tgctcggtcg gggaggagcg
     1441 cgacgagcca gtcgctcgca tcgacacggc tgtagcccgg caaaccgcgt tcgaagccag
     1501 aggcggcggg tatagccctt ggaacctcgt agtgagccgg gtccaaggga aggagatcaa
     1561 atggcacgag ccaagtcttc gagctcgagc cggaattcgc ggtcgaagag cgggagttcg
     1621 cggtcgaaca gccggagttc gcggtcgagc agcggtaacg gcaagtcgtc ctcgaacgcc
     1681 aaccgctcca aggaaaaact gccggactac tccgtcatcg acccggatca gatcgaacag
     1741 gcggtcgacg tctacgtcga cgtgcccgtc gtaaacgtcg acgagatcaa gttcgagctg
     1801 gacgatctcc gagcgcacgt cgccgtgctc gccgaggtgg ggcacttcgt gcagctgaac
     1861 gccggcgtcg cggcgaggct cggcaacgtc gagctcgaca tcaagggagt tgagacgcag
     1921 gcgctgctcg aggcgcgact gcacaacgtc acaaagattc tggggcgcgt cttcacgtcg
     1981 ctcgaccgca accccgagct gctcgagagc gtcggccagg cgcttggcga cgtgggcggc
     2041 ggagcgcacg atctcatatc ggacaccggc gacgtagtga agagcgtcgg caagggaggc
     2101 gagagcgccg tccaggacat cggcaagggc gcggggcgtg gagttgaggg catcggccgc
     2161 ggcgccgagc agggcgtcca gggcttgggc caaggagccc agcagggtgt cgaaggcctc
     2221 ggccaaggcg ccgcccaagg cgtcgagggc ctcggccagg gcgccgccca gggcgtcgag
     2281 gggctcggcc agggcgcgca gcagggagtt gaaggcctag gtcagggcgc cggacagggc
     2341 gttgaaggcc tgggtcaggg cgccgagcag ggcgtcgagg gcgtcggcca aggcgtccag
     2401 cagggtgtcg gaaatctcgc gcagcagggt tgaggagtca ccgatggctc agcagacaaa
     2461 gtcacagcgc tcggggagaa gccgtagtca gaacagctcc tcccgtaacg gaaagggcga
     2521 cgctcgtagt gggaacgggc tgacgcctgc gcagcaggct cggcgcgagg tgagccaagc
     2581 cgaccgcgag taccggaagt acctgttcga ggaggagcgc gaccggacgg ggccgcccga
     2641 cgtcgtgctc gacgtgcccg agctgcaggt cgacctgatc cacttcgagc tcgacaatct
     2701 cgatgctcac gtggcgctca aggcgaaggt gctcaacctc gtgaagctca acgtgggcgc
     2761 tgacgtgcac ctcagcagag tcaagctcga catcaagggc gtccacgcgg aggtggtcct
     2821 aaaggcgcgg ctcgatcacg tcgcggcgat cgtggaccgc ctgatgacga cgctcgatcg
     2881 caatccggat ctgatcaagg gtctgagcca agcagtcagc gaggtcggcg ccggcgcggg
     2941 tgaggcggtc gacaagaccg gcgatgcggc caaggacatc ggacagggcg cgcaaggggc
     3001 cctgaaggac gtcggcaagg gcgccggtca ggcggtcggc gacgtcggtg agggcgccgg
     3061 tcaggcggtc ggcgacattg gcgaaggcgc cggccacgcc gttggcgacg tgggtcaggg
     3121 cgccggccag gccgtcggtg acgtcggaca aggcgccggc caggcagttg gcgatgtcgg
     3181 tcagggcgcc ggccaggccg tcggcgatgt cggacaaggc gccggacaag ccgtgggcga
     3241 cgttggccag ggcgccggcc aggccgtcgg cgacgctggg cagggcgccg gtcaggcagc
     3301 cgggaacctc ggcgggctag ttggaggcgt cggccagacc gtgggccagg cgggccaggg
     3361 cgcgaccgaa gcgctcggcg gtgtcaccca gggcctcggc ggccagcagg ggcagcaggg
     3421 ccaacagggg cagcagcagc agcccgcccg ggagggccag cctgaccagc aaggccagca
     3481 gcagcccgcc cagcagggcc agcctgacca gcagggccag cctgaccagc agggtcagcc
     3541 tgaccagcag ggccaacagg ctcagcccgc ccagcaggac cagccgcagg gcgctcagga
     3601 ggagacggcg cagacgaacg gcggtggccc aaacgcggca aagcccagct cgctagccaa
     3661 ggaggtcgcg aagatggtcg cccgtcaaat tgggcaggcc gctcaggacg aggcccgcga
     3721 cctcggattc gcggcgactc gcaaggtgaa ggagctcggc gagcggcgtg agcagaggcg
     3781 cgccgagaag taccacgcca ccgaggctgc gatgcggctg gccgacgagc tcggcgtcga
     3841 cctggcatgg tgcgatggca cgggcagcga cggcaagatc accgtgaagg acgtgcggga
     3901 ggcagtcgag gcgtgagccg ccgaaagcga gagcccgacg tgcagatcgc ggcgtcgatc
     3961 aaggctgacg agctgcgttt cgaggtcaag ccggacgttg aggtccgcgc gtactccaac
     4021 tccgacgctg aggcggaatc ggtctcagag cgcgagaacc tgccagacga gctggagccc
     4081 ggcgtcacct accgcgactt cgcggtgcgc tggcgactcg cggctcgcct gaaggatccg
     4141 gacgaggacg aggggtagag gggcaacgcg ctctgctacg agagcgcgtt gccgatgtcc
     4201 tcagccttct tgcgggtggt ttgcacctca ctcgcgagct tgccgaactg cttgccggcc
     4261 ttccggactt ccttggtgag accgttcatc ccgctgccgg tccccgggat cgggacgccg
     4321 agcaccttgc gcggccgctt gtgccgccgc tccaggacca cgccgcccac gatgcccgcg
     4381 gccgccgtca cggccggaac cgcgaggttc ttgatcgtcc cggcgacacc gccgccgccg
     4441 ttgcgctgct gcgcggagct cgcggatgcg ttctgccgcg agcttgcgga gctcgagcgt
     4501 gagctcctgc ttctcgaggc cgagctcccg ctgctcctgc ggttgggagt ggatgcgttg
     4561 ccgctgccgc cgttcgacga gctgcgcgac gacctgctct tggagctcga ccgcctggtt
     4621 gtttcagcca ttcttgctct ccctccggtc gtgtacctca cttgcgggat acccgcggta
     4681 tggaggacgc acacagggaa cgcgtcgaat gagcgcatgc accggccccg gcggttcctc
     4741 gtcagcgcgc gaacgcggcg agccgctgtg ccaattggga gtggacgcgt gcggcgtgct
     4801 cttcgcggtc cagcgcggct cgataggcag cgaacccgag ccggcgttgg gtggggttgc
     4861 attcggtcca gcgacggtat gccgtgtgaa gttcatcgca cgcctcgcgc cagcacacgt
     4921 agctctccag gaacgcgtcg gcgaggatcg accgcctttc agtgagaggg cccaccatga
     4981 ctcccggttt atgccttcgc gctgaccatt ggcggcgtcc ttgactgccg atccgcgggg
     5041 tggttcgggc gcgctagtag ccgaggcccc ccgatgagcc ggctgagccc tggccggaga
     5101 cctggttgcc cgccgcggtc agcgcgaacc aacttccgcc gaaagccgtc agtccctgcc
     5161 cgttcgtgtc gcccggcttt ttgtcgccaa cgaagcggta gagcggatgg cggttgtagg
     5221 tgacttgcgg ctttccgtca gagcgacgga tggttcccac cttggaggcg ctcgccccgg
     5281 cgcccaccgt cggcttccgg ctcgaccgca gcggcggcca gttcgtggcg caggcgccga
     5341 agcaggcgct cttcgcaccc gagtccttct tgaacaggta aagcgtgtgg ccccgcgagt
     5401 cgacaaggat cttgccgagg gagccctcgt ttgcgccgct tacggtcgcg gcgtggccgg
     5461 aggccgtcgt gggcggtgaa ctcgcagtgg cagcaccacc gccgccgccc ccgcagccgg
     5521 ccgcggccag cgcgacaagc gccaccgccg cagcggcacc gagcgaggag atcgttccgc
     5581 gtcgagtcat gctcgttcct tcggtactgg gggttgcgtc tcggccctcg ccacaaagcg
     5641 gcgccaagct ctcgaatcac gcggccatgt tggccgcggc gctcacactt gtcgtcggac
     5701 cgcggaggac tcttccgcct actgcgcgcg tcgtagccct agcggcggcg tacggctgtg
     5761 atggcgctcg aaacctgatt cggcgcggtg gcggtttggt agttcaccca cgggtcgatg
     5821 gccacacata cccccctcag aggacgaggc gaagagctgt cagcgcagcg gacccgccgg
     5881 gtgttgctcg gccggcaaag agagtgcgag acgctccacg gcgtcctcga cgccgttcgc
     5941 gggggtgaga gccggtcgct cctactgcgc ggggagccgg gggtcggcaa gacggcgctg
     6001 ctcgagtacg cgatcgagtc ggcccccgac ttccggatcg ctcgggccgt gggtgtcgag
     6061 tctgagatgg agctcgcgtt cgccgccctc catcagctgt gtgcgccgat gctcggccga
     6121 ctcgatgcgc tgccggtccc tcagcgccac gcgctccgtg tggcgttcgg gctgaccgtg
     6181 ggcgacgcgc ccgaccgctt tttcgtcggg ttggcagcgc tcagcctgct ctccgcggcc
     6241 gccgaggagc agccgctcct gtgtgtcgtc gatgatgcac agtggctcga ccgtgcctcg
     6301 gcggaagcgc tcgcgttcgt tgcgcgcaga ctgctcgcgg agtcggttgc tgtggttttc
     6361 gcgacgcgca cgacatccga ggactggagg ggcgtgtcgg acctggtcgt cgagggtctg
     6421 cgagatggtg atgcgcggga gctgctcggc tccgtgctcc gggggccggt ggacgagcgt
     6481 gtgcgcgagc ggatcgtcgc cgaaacgcgc ggcaaccccc tcgcgctctt ggagctgccg
     6541 cgcgggttga cgcccgcgga gcttgccggt ggcttcgggc tcccggacgc actgccgctt
     6601 tcgggtcgga tcgaggaaag cttccggcgg cggctcgagg agctctcggc cgagagccgg
     6661 ctgctgttga tcgtcgcggc ggcggaaccg ctcggcgagc ccgtgctcgt ctggcgtgcc
     6721 gccgcgcggc tcgggatcgc ggccgatgcg gcggcgccgg cgtccgcggc cggcgtgctc
     6781 gagaccgggg cccgggcgcg gtttcgacat ccactggccc gctcggcggt ctacaaggca
     6841 gcttcaccgg gggaaaggca gcgcgcgcac cgcgcgctgg ccgaggcgac cgaccccgag
     6901 gtcgatccgg agcgtcgcgc ctggcaccgc gcccacgcga ctacgcggcc cgacgaggac
     6961 gtcgccgccg agctcgagcg ctcggccggc cgggcacagg cccgcggcgg tctcgccgcc
     7021 gcggccgcct tcctcgaatg cgcgaccgcc ctgacgcccg agccggcgcg ccgtgtggag
     7081 cgcgcactgg ccgcggcgca ggctaagagc caggccggcg ctcccgacgc agcgcgggaa
     7141 ctgctgagcg ccgcggaggc gggacccctg gacgcgctcc agcgtgttcg ggcggaccgg
     7201 ctgcacgccc agattgcgtt cgccttgaac cgcggcagcg acgctccgcc gctgctgctc
     7261 aaggcggccg agcgtctcga tcggctcgac gtgagactgg cgcgcgagac ctatctggaa
     7321 gcgttgacag cggcggtgtt cgcgcaccgt ttggccaccg ccggaggtgt gctcgaggtg
     7381 gccgaggccg cgcaggcggc gcctccgtcg tcggagcccc cgcgcgcgcc cgatcttcta
     7441 ctggatggct gggtgctgct gatcaccaag ggccgcccga cggcgacgcc gacattgaag
     7501 cgggcgctta gcgcgttccg gagcgagtcc atctccaagg aggaggagat ccgttggctc
     7561 tggctcgcct gccacaccgc ctttgaactc tgggacgacg agacctggca cgtcctctcc
     7621 gcccgcctgg tcgacctcgc ccgcctcgct ggcgcgctca cagagctccc cgtcgcgctc
     7681 gactcgctct ctgtagcgaa cctgttcgcg ggccgcttcg cggtggccgg atcgcttctc
     7741 gacgaggcca aggcgatcac cgaggtgacc gggagccacc ccacgcccta cggtcctgtc
     7801 gtgttcgcgg cctgccaggg ccgcgaagcc gatgcctctg agttgatcga ggccagcacc
     7861 aaggaggtgg tgcgccgggg tgagggaatg gggctgaccg tgattcgctg ggcaagcgca
     7921 ctcctctaca acggcctcgg tcgctatgac gacgcactgg ccgcggccca gctggccagc
     7981 gcgcatccgt tcgatcccac ccggggactg cccgagctca tcgaggcggc cgcccggagc
     8041 ggtttgccgg agcgggcttc cgatgccctc caacggctcg ccgagacgac acactccagc
     8101 ggctccgaat gggggcttgg ggtcgaggcc tgctcgcgag cggtgctgag cgagggcgcg
     8161 gtcgccgaga acctctaccg agaggcgatc gaccggcttg ggcgcacccg cgtccgcttg
     8221 gcactcgccc gcgcccatct cctctacgga gaatggctgc gccgcgagcg ccggcgccag
     8281 caggctcgcg agcagttgcg cacggcccac gagatgttca cggccatggg catcggggcg
     8341 ttcgccgagc gcgccgcgcg cgagctgcgg gccaccggcg agactgcgcg caaacgcacg
     8401 attgagacca gcgaccagct aaccgcccag gaaattcaga tcgcgcgact cgcacgcgac
     8461 ggcctttcga atcccgagat cggcagccag ctgttcatca gcccacgcac cgtccagtac
     8521 cacttgagca aggtcttcag caagctcgcc atcagctcac gagcgcagct cgacgacgtc
     8581 ctctccagcg atcgcgacct ggtgggcgtc agccagcgag cggaagaggt tgccattcgg
     8641 cgaccggagc tttagcgcgc ggcaccacga gcccggtctc gtaggcgaac acgacgagct
     8701 tggcccgatc gtgcgcggcg agcttcagca tcgcgcggct gacgtgggtc ttggcggtcg
     8761 cacggctgac cacaagccgc tcggcgatct cgtcgttgct cagaccgagc gccacgagca
     8821 ccaccacttc ccgctcgcgg gcggtgagct cgtcgacaag gttcgagctt ggacacgagg
     8881 gctcctgccg cgcggcgagt tcggcgatca cgcgccgcgc gaggctcggc gagagcgccg
     8941 cttccccgtc ggcgacgagt ttgaccgcgc gcacgagttc gggtggttcg atgtccttga
     9001 gcagcaggcc gctggcccca gcccggagcg cgccgaagat gcgttcgtcg tcctcggagg
     9061 ccgtcagcag catcaccgcg acgctcgatt cggagagcat ccgtctcgta gcctccacgc
     9121 tgccgatccc cgagagctgg gcgtcgatca gcgcaacgtc gggacggatg cgacccgcca
     9181 gcgcgaccgc ttcctcaccg ctggtcgcct cgccgacgac tcggatgccg tcagcagact
     9241 cgagcagcat tcggaagccg gcgcgcacca gtgactggcc gtaggcgatc agcacccgca
     9301 cgtgtccggg ctcctccggg gggacaatgg cgagtctgcc gggcggacgg gctccggcgt
     9361 gtgggtgcgg ctggagtgca tgtggcatac ggcgagtgtt gcgcggccgg cacccgttcg
     9421 catctgccag gcgacagtgc gagtggccag tctggcgggc gtaggcccgg tcgaactacc
     9481 gcgccggcgg tacccagaag agtcgcctgc gaggcgacga caagcgcacc cccgctcgtg
     9541 accatgccgg atcgaacctc gaaccggagc gcgccaatga tttcgtccgt tacggcccag
     9601 ggggctcggc cggtgaagat ccttgcggtt ctcgcgggcg cgctcctggc gatcgcagcc
     9661 ctggcgggaa ccgcccacgc atccggcccg ggtcagccgc cgcctgagtg ggcagcgaat
     9721 gcgggtgagt ggccggcgca caactacgac ctggcaaaca cccgcgcgac tgcgcagtcg
     9781 ccgatcgatt cccaaaccgt ctcgcggctg aaggtgaagt ggaccttccc gctgtcgggc
     9841 tcaggcgcgt tcggcgcctt ttcctcgacg ccgatcgcct tgaacggcac ggtctatttc
     9901 caggacctga actcgaacgt gttcgcgctc gaccgctcga caggcgtccg gaagtgggag
     9961 cacaagttca acaggccgag cgccggcccg aacggcatcg catttggcaa tggccgcatc
    10021 tatggggcca ccgagacgag cgcgttcgcg cttgacgcgc gcaacgggca gctcgtctgg
    10081 agccgcaagc tgatccgcaa taaccgtgag ggcatcgaca tgacgcccca gctctacgac
    10141 gggaaggtgc tgatcagcac gatccccggc aacgcctccg tcttctacaa gggtggtgca
    10201 ctcggagtcg tgtggtcgct cgatgccacc accggcaagc cgaagtggaa gttcaacacg
    10261 atctcggacg gcgccaagct gttcggtaac acgaaggtca acagcggcgg cggcctgtgg
    10321 tacccgccgg ccgtcgacga ccaggggcgc gtgttcatct ccgtcgccaa cccggcgccg
    10381 ctgtacggca cgaagcagtt cccgaacggc tcgagtcgcc caggccctga cctctacacc
    10441 agctcgctcg tggcgctcga tgggcaaacg gggaagcggc tctggttccg gcaggccgtg
    10501 ccgcacgaca tccgcgacta cgacctgcag atccccgcta tcacgaccac cgtccaactc
    10561 ggcggcgtcc ccaccgaggt cgtgctcgtg gccggcaaga tgggcaaggt ctacgcatac
    10621 agggcggccg acggcaaggc gctgtggact cgctcggtcg gcaagcacca aaacgacacc
    10681 gggccgctcc cgcgcaagct cgtcaccgtg ttccctggtg atttgggcgg ggtcgagacc
    10741 ccgatggcgc tcgccgccgg gcgcctgttc gtgccctggc tcgacttccc ggtacgcgcg
    10801 cgcgcggagg gtttgccggg cggactggcc ggcactttga agaacttcaa ggtcggccgc
    10861 ggcggcttca ccgctttcga tgccgcgacc ggcaacgtgc tctggcagcg gaagttgccg
    10921 acgatggatt tcggcgccgc caccgtcgcg aacgacgtcg tcttcaccag cgactacgcc
    10981 ggcacggtct acgccttcga cgcccagacc ggcaagaccc tttggaccgc caaggcgccc
    11041 gccggcatca actccttccc cgccatcgac ggcgacaccc tgctcgttgg cgcgggcacg
    11101 gccagcttcg acaagaagcc caagttcgag ctcgtcgcct actcgctcca gtaaccgacc
    11161 cttccaagga ggactctgat gagacgacta gcgaagcgat cgacgctcgc gttcgtggtc
    11221 gctgcgatcg cggcgctggc gctcgtccag ttggcgctag gagccgcagg gccccgcgcg
    11281 agctcgacca ctgaccaagt gcgcgggaag gagttctcct tcaaactctc gaccaggtcg
    11341 agccggcgcc ctggcaaggt caggttcact ttccgtaacg tcggcacggt ccagcacgat
    11401 ttcaggatca acggcaagca gacaccactc atccgcccgg gaaagagcgc gagcatcgtc
    11461 gtgaacttca agaagccagg aacctaccgc tacatctgca cggtgcccgg acacgcggca
    11521 gcgggaatgc gcggcgtctt caccgtgcgc tgatcgtaat cgcgcagccc tgcgcgtgcc
    11581 agagcgaagg agcatgagat gctgaaagaa ggcagggtcg cgacgaggct tccggcgcag
    11641 gacctgaatc gggcgaaggc gttctacgcc gacaagctcg ggctccaccc cgccgaggaa
    11701 cgggagggcg gcctccggta cgtgtgtgcc ggcggcgagt ttgcgctttt cgagtcggcg
    11761 ggcgccccct caggcgagca cacccagatg ggatgggaag tggacgacat cgatgcgacg
    11821 gtcgccgagc tgcgctcgca cggggttgtc ttcgaggagt accacgcgcc cggcctcgag
    11881 accgtcgacg gcatcgcgga catccgcggc aactacccga gcaaaggcgc cggggagcgt
    11941 gccgcttggt tccgcgacag cgagggcaac atgctgggga tcggacagcc ggttcacgcc
    12001 taggaggtat ccccaaaggg acctgactag gctccgagca tgatcatcac aacgatgaac
    12061 gacctccctg gctaccagat cgacgaagtg atcggcgagg tgttcgggct caccgtacgc
    12121 tcgcgcaaca tcggctcgca gatcggcgcg tcgttcaagt cgctagttgg cggggagctc
    12181 aagggcatga cgaagatgct cgccgagggc cgggcgcacg ctcaggatcg tctggtcgaa
    12241 gaggccgagg ggaagggcgc gaatgccgtg atcgccttcc gtttcgacac ctccgagctc
    12301 ggcaccacct ggaccgagat ctgcgcctac ggcaccgccg tgcgcgccca caagctctga
    12361 acggccgctt cgcgccgagc cccacgggcg agctgcacct ggggaacctc cgcaccgcac
    12421 tgctcgcatg gctgttcgcg cgctctgcag gctcgcgctt cctcatgcga gtggaggacc
    12481 tcgacccgca gcgctcgaag cccgagcacg aggagcggca gctcgccgac ctgcgtgcga
    12541 tcgggctcga ctgggacggc gacgtggtcc accagtcgga gcgcaccgag ctctaccgcg
    12601 acgcgctcgc gcggctccgc acgcaggggc gcgtgtacga atgctggtgc acgcgtgccg
    12661 agatacgcga agccgtgcag gcgcctcacg gcccgctgcc ggaaggcgcc taccccggca
    12721 cgtgccgcga tttgaccgaa gacgagcgcg aggagcgccg ccgctcgggg cgccctgctg
    12781 cgttgcgcct cgacgcacgc ggcgacgccg tccacttcca cgatcgcctg cacggcgcgg
    12841 tgatcggcac ggtcgacgat ctagtgctgt ggcgaggcga cggcactccc gcctacaacc
    12901 tcgcggtcgt ggccgacgac gcggaccagg gcgtcgagga agtcgtgcgc ggcgacgacc
    12961 tgctcgactc cacgccgcga caactgctgc tggcgcggct gctagggatc gcggcgccgc
    13021 agtacgcgca cgtgccgctc gtgctcgcgc ccgacggcag ccgcctcgcg aagcgccacg
    13081 gcgcggtcac gctcgccgca atcgggcccg aggacgcact gtcatggatg gctgcgagcc
    13141 tcgggatggc ggctcccggc gagcgcgtga ccgctgccga tctgctcgaa cgcttcgacc
    13201 cggacgggct gccgctcgag ccgaccgtgt tcagcccgag cgccgccgct cgctgaccga
    13261 gcgcagcagc gctcgcacgt ccgcgacggg cccgaggtgg tcgagcttgt cggggttcac
    13321 gatcgagttg atcccctgta tccggccctc ggcgacgtcg agcgacatca cgttcacgat
    13381 cgcgccctcg gtgtcgagcg tcattgcgcc tggctgcccg ttcacctcga caatccgcat
    13441 gtccaggtcg aagccggcca tctgccgcac ccagccgagc agcaccttgg cgacccgctc
    13501 acgaccatgg atcgggcgcg cgagcgccgg cgccttgccg ccgccgtcgc cgtgcagcac
    13561 gacgtcctcg gcgagcagcg cctcgaggcc ctcgaggtcg ccctcctcgg cggccgcgaa
    13621 gaatttccgc gccagctcgt cgcgctcctc gcgggaggcg tcgaagcgca gccggcgctc
    13681 gtccacgtgg cgcctcgccc ggactgccag ctggcggacg ttgtcctggc tcttgtcgac
    13741 gatcgcggcg atctggtcgt acgggtagtc gaacacctcg cgcagcagga acaccgcgcg
    13801 ctccaccggc gagaggctct ccagcagcac gaggaacgcg gtagagagcg agtcggccac
    13861 ctcggcgtgg cgactcgtct cgtcgccgga ggtgaggagc ggctcgggca gccactcccc
    13921 cacgtaggtc tcgcggcggg cccgagcgga gcgcagctga tcgatcgcca gccgcgtggt
    13981 ggtcgtcgag agccacgcct tcggcgactc gacctggcct cgctcgcgat gcagccggag
    14041 aaacgcctcc tgcacgacgt cctccgcctc gctcacgctt ccgagcatcc ggtaggcgat
    14101 cgagaacatg agggggcgaa ggtcctcgta gacgtcggta gtcatgcggc gagcacctcg
    14161 gggaacgtcg tacgccaact cgagtgccgg ggctgccacc cgagttccct cttcgccttt
    14221 tcgttcgatg cgcctcgcac ctcggtcatc atgacaacca cggcgtcgcc ggcgagcacg
    14281 cgcccgagcc acaacgggaa gtggcgaggc ggcttcgcgc cggcggcttt cgccgcgact
    14341 ggcagccact ccgacacgcg tgcgggctca tcgtccacca cgttgtagat accgcggcgt
    14401 ccgtgctcga tcgcctccac cgtcgcggcc gcggcgtcct cgatatggat gaacgaccag
    14461 atcccgctgc cgctgccgac gatcgggaac tggcgtttgc ggatcgcctc ggtgtagtcg
    14521 ccgtggcgcg acagcgacgt gcccggcccg tagaagccgc cgtagcgaag agcgattccc
    14581 tcgatgcctt cggcgcccgt caccacggac tccaggtgct tgatcgcgtc gagggtggtc
    14641 ttgaacttcg gcgagggatt tggatcgagt ggagcgtcct cgcccttgac agccgatccg
    14701 tttcgctcgg acggccagcc ggcgaagctc tgcgccacga agcgcttcgc gcttgacgcg
    14761 agcgccgccg ccagcaggtt gtctgtgccc tccgtccgca gccggttggt catcgcgaag
    14821 tcgcggtcga aatggcgcag gtcgagcgag ccggacaagg cggtcgcctc gtgcaccaca
    14881 acgtcgggcg aggcattgag caccgcctcc cgtacagccc tggcgtcgag caagtcgagc
    14941 accaccggct tcgcgcccag gctgcgcatc aggtccctct tggcctgcga ggtggtggtg
    15001 ccagtgacct cgtgaccgcg cgtcacgagc attggcacca gctgctttcc gagagcgccg
    15061 cctgcgcctg caatgaacac cttcatgtcg tcctccttcg tctgcatgtc gtgcatgaga
    15121 cgagacagcg gcccggcctg tgacaatcac tcgcgatggc cgagctgccg cccgaagtgc
    15181 gcgccctgct cgaggcgcct aacttcgtgc acctcgccac agtgaaccgc gacggctcgc
    15241 cctcgagcgt cgcggtgtgg ggcatgctcg agggcgggcg cctcgtgttc ttcacacagc
    15301 gtcagacgca gaaggcgcgc aacctcgagc gcgacgcgcg cgtggctgcc tcgatcatcg
    15361 atcaagagga cccgtacaag acggcgcgcc tgcgcgggcg ggtcgtggag aaacgcacgg
    15421 gacaggaggc ggccgatacc atcgaccgca tctcgcgggg gtacatcggc gaggccttcc
    15481 ccttccatac ccccaacgag gtggtgttcg cgatcgagat tgagcacacc tcgttcgtgg
    15541 aactcccctt cgagcacacc cccaggagct gacttgcacc acccgatctc ttacttccag
    15601 gcgatcatcc tcggcttggt gcagggcgcg gccgagccgt tcccggtgtc gagcctcggc
    15661 cacggcgtga tcctgccgcg gctgctccac tggaacatcc accagaacga caagttcttc
    15721 ctgacgttcc tggtggcaac gcacctcgcc acggcgatcg tgctgttcgg gttcttcttc
    15781 aaggactgga tgctgatcct gggcggcatg gggcgctcgc tgcgcgaccg ccagatctcg
    15841 gagggcgaca catacgcgcg actcggttgg ctgctcgtcg tcggcacgat cccggcgggc
    15901 gtgatcgggc tgctgctcga gcacccgctg cgcaagctct tcgcctcgcc gcagtcggcg
    15961 gcagcgtttc tgatcgtgaa cggcctgctg ctgtttgcat tcgagaagct ccgccgccgc
    16021 ccgccccgtc ccggcgact
//
